LOCUS       DAOSAS010000001        45013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_619_length_45013_cov_8.833689, whole genome shotgun sequence.
ACCESSION   DAOSAS010000001 DAOSAS010000000
VERSION     DAOSAS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 45013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45013
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_619_length_45013_cov_8.833689"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            947..2545
                     /locus_tag="PLR72_00005"
     CDS             947..2545
                     /locus_tag="PLR72_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6377 domain-containing protein"
                     /protein_id="HOK35631.1"
                     /translation="MKNVFLILLFIICPITVFSTINKTDSLLKVLDKVISERDSYLKI
                     KEEKILELKKKKQQSKTLEDQININREIIDEYNSFICDSAEFYINQNLLIAKQLNNKE
                     LILENYFRLSYIYSLSGLFLEAAEIFNSINYESLSTGYKAWYCWNRIRFYENLINYTA
                     DTKFSKQYEKEKEEYREKVMGLLYNQSDVYKKELACKLETEGRYEEAINLLTPIFYKQ
                     QPETHDYAMAAMNLAKVYKLAKKYDEEEYYLILAATTDIKLAVKENEALLSLAINLYN
                     REDVNRAYSYVRVALDDALFYNARYKNSVIGRVQPIIEDTYLQKIKYQQKNLRLYSML
                     TSVFIVILAFFLFFIYKQNKALSKARKKLREVNEDLLHLNQKLNEANIIKERYLGYFM
                     NQCSIYINKLYKYRKEVNLKIKTGQINSLYKSSTSELEKEIEELRTNFDKAFLKLYPN
                     FVSEFNSLLKPEERYELEDGQLNTELRIFALIKLGISDVSQIADFLHYSSQTVYNYKS
                     KVKSKSVIESDQFEEEVKKIGKLQ"
     gene            2877..3401
                     /locus_tag="PLR72_00010"
     CDS             2877..3401
                     /locus_tag="PLR72_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOK35632.1"
                     /translation="MERDFSNIYMTFYSPLVRFAMVFVIYKEEAENIVQDLFVGLWEK
                     KESLNCVENLKAYMFTLAKNKCLDYLKHKLVKENFIAKAQSFYEQEIALKAQSLESFD
                     VELASEEKVEKIVSEAIESLPPKCREIFILSRYEGLKYKEISERLNISVNTVETQMRI
                     AFKKLRNSINYLLN"
     gene            3478..3714
                     /locus_tag="PLR72_00015"
     CDS             3478..3714
                     /locus_tag="PLR72_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35633.1"
                     /translation="MINLAIKYLNGELTSEEKERFFVLVAKDETLKNDLIKYHHLLAY
                     ISLLFRKEDNADTERRFLDLLNEIERKKRKEKDG"
     gene            3707..6241
                     /locus_tag="PLR72_00020"
     CDS             3707..6241
                     /locus_tag="PLR72_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOK35634.1"
                     /translation="MVKVKVRITWTLLVLSIFLNAENVYFVDGYHGGVYGHYPLEWET
                     RFIIDNFYKYPDWRICLEIEPETWDSVKVRTPQDYTQFKQILSDERIEFTNPAYAQPY
                     CYNISGESIIRQFQYGMRKINQHFPDVTFSTYSSEEPCFTSCLPQLLKSFGFQYAVLK
                     CPNTCWGGYTRAYGKDLVNWVGPDTTSILAVPRYQCESFEKNSTWQTIAWNNSTSYIN
                     TCLEADIKNPVGMCFQDAGWKNGPWLNSIKNADPNSIYVTWKEYFEKISDGKSTDNWQ
                     FSQEDVLVSLMWGSQVLQKIAQQVRNAENKIIIAEKMGSLAYLKNNFHYKQDSIDEAW
                     RTLMLSQHHDSWIVPYNRLQKNTTWADKIAQWTETTDKIADSVVKEAVESFQGNNKSV
                     NDDYSIRVFNTLGFNRNEIVSILLPETLKNKKVILLDTENQVLPSSVEEGQKISFLAQ
                     VPAFGYATYKIQMGESKIKKEKKTFYSNKNECILENDKYRIVFDLQKGGTIKSLFAKN
                     NNKEYVKKGSEFLFGEMRGFFYEENKFHSSRENPAKVTLIKDNSLETSILIQGKIASQ
                     PFKQIVTVNKEQERIDFDLVINWKQNLCIGEYAQPNNWQDNRRAFYDTRYMLSVLFPV
                     DLNSPQLYKNAPFDVCKSKLDNTFFNRWDSIKHNIILNWIDFSEVNGENSLALLTDHT
                     TSYSYGTDFPLALTIQYSGKGLWGRDYNITQPTHVKYALVPHKFRWDKSSISKKSSSW
                     NEPLIGVCSSDFDFENKSFIDLLSSGYELSSVQIMNDGILVRLFNSEGDGSAQKIKLC
                     FPFKSIEEMELNGEMKNANGSIKAILGNEIQLSIPRFGVRSFYIKN"
     gene            6326..8665
                     /locus_tag="PLR72_00025"
     CDS             6326..8665
                     /locus_tag="PLR72_00025"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOK35635.1"
                     /translation="MYKFFWSNVSVFLFLLFTSCGVKNSDKEKIRPVDYVNPFIGAST
                     SIGDAGIYHGLGKTFPGATTPYGMVQVNPNTITGGDNAPGYSYEHKTIEGFAFTQMSG
                     VGWFGDFGNFLVMPTTGELYKIAGKEDGSIKGYRSRYDKKSEIARAGYYSVKLSDYKI
                     KVECTATPHCGILKFIFPENKKSRIQIDLAHRSGGTSTYQYIEIVDKNTIKGWMKCPP
                     SGGGWGNGEGKADYTVYFYAQFSKPLEEYGCWSVNIPDNWKRKKDEVVSIPYLKRVAE
                     APVFRKFKKLEGKHLGFFTEFPTNKNEIITLKTGISFVDMAGAEKNFNAEIKNKSFDQ
                     IKADAQNLWNEELSKITISGGTEDEKTVFYTALYHTMIDPRIYTDVDGRYVGGDLKIH
                     EKDSTFTKRTIFSGWDVFRSQFPLQTIINPQLVSDEINSLTTLAEQSGRKYFERWEIV
                     NAYSGCMIGNPAISVLADAYVKGIKSYDINKAYEYALNSSAKFGNDKFGFAATPFCIS
                     ETLEYAYTDWCIAQLAGKLGKTGDEKLFLSKSQAYKNIFDADKSWFRPKYSNGLWKTW
                     PKEGRLRQGYGCIESNSYQQGWFIPHDVNGMVEIMGGKEKVLKDLNSFFENTPSNLRW
                     NNYYNHANEPVHLVPFLFNRIDVPWLTQKWTRFICRQGYTNKVEGLVGNEDAGQMSAW
                     YVLASSGIHPSCPGNTRQEITSPVFNKIEFKLDPKYFKGKKFTIIAHNNSPKNIYIQR
                     AKLNGKEYNRCYIDFADISSGGTLELFMGEKPNKSWGIN"
     gene            8683..9153
                     /locus_tag="PLR72_00030"
     CDS             8683..9153
                     /locus_tag="PLR72_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35636.1"
                     /translation="MKRCFYVIVLLFCFLFARFDFIYADGIGRPWNALWIGPKNDKGT
                     EYGVYYFRKSITIKEKLESFNIHVSADNRYKLFVNGKVVSMGPARGDTYFWNYETVDI
                     APYLVSGNNCIAALVWNEAEYRPEAQISIRTAFILQGNSEKEEILNSNETWKCI"
     gene            9217..9456
                     /locus_tag="PLR72_00035"
     CDS             9217..9456
                     /locus_tag="PLR72_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35637.1"
                     /translation="MAKTVKGDWISVSYDDRNWPNAVKILEGNLKGKSDGLAWQLVPS
                     SLPQMELTYQRIPKLRWAKGMNAPLGFQKLKLNLI"
     gene            9396..9638
                     /locus_tag="PLR72_00040"
     CDS             9396..9638
                     /locus_tag="PLR72_00040"
                     /inference="COORDINATES: protein motif:HMM:NF036579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOK35638.1"
                     /translation="MGKRDERSLGFSKIKIEPHLGTTTKVGGEMPHSNGKISVNYELK
                     KKKWIIHVHLPQNTPGKLIWKGKNYELNPGDNSLVI"
     gene            9683..11968
                     /locus_tag="PLR72_00045"
     CDS             9683..11968
                     /locus_tag="PLR72_00045"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOK35639.1"
                     /translation="MKYRLLALISCSFIMLSTLKAQQKDLVKYVNTLQGTDSNFGLSY
                     GNTYATTAMPFAMHTWAAQTGKNGDGWKYQYSADRIRGFQQAHQCSPWMGDYAVYSLM
                     PEVGKLVVNQDSRASKFSHKNEMAKPHYYKVKFDNGIITEIAPTMRGVHLRFTYPKNE
                     DAYLVLDGYIDKSEINIDPTKRQITGWVNNQRFVNNASTFRSYFVIQFDKPFEEFGIW
                     ENNKDEIFAGKTSGSGKGYGAYVKFKKGAVVQAKAASSYISYKQAWITFNRELGKDKK
                     IEMTMEKGAKTWNDLLNRVLVEGGTEEQMKTFYSCLFRANLFSREFYELDANNEPYYY
                     SPYDGKIHKGYMFTDNGFWDTFRSQFPLTNILHPTMQGRYMNALLDAQEQCGWLPSWS
                     FPGETGGMIGNHSISLLTDAWAKGIRTFDPERAMKAYAHESMNKGPWGGSNGRAGWKE
                     YWQLGFVTYPESMGSTSQTLEYAYDDFCGYQLAKMTGNEFYENIFAKQMYNYKNVFDS
                     KTGFMRGRDKNGKWLEPFNPYEWGGPYCEANAWHYTWSVFHDVQGLINLFGSDEKFTT
                     KMDSVFTVPNIIVPGTYGGVIHEMKEMQLANMGQYAHGNQPIQHMIFLYSYAGQPWKT
                     QYWSRQIMDRLYNSTEKGFPGDEDQGGMSSWYVLNALGIYSVCPGTDEYVLGSPLFQK
                     ITITTEDNKKFVIEANNNSKENIYIQSVSLHGKPLEKNYIIYSDIVNGGILHFEMGNQ
                     PNKSRGISKQAVPFSLSNNKF"
     gene            11973..14129
                     /locus_tag="PLR72_00050"
     CDS             11973..14129
                     /locus_tag="PLR72_00050"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOK35640.1"
                     /translation="MKVKVYFLCLLSLSICSSISSSQNLNINYANRVNTLIGTGGFGL
                     KSGNCYPGATYPFGMVQFTPTYFSKNLGFVINQLNGAGCEHMGNFPTFPLKGKLKISP
                     DNILNSLINLSKEEGHAGYYKAVVEEEIVAELTVTERTGMARYTYPSSEEFGTIIIGA
                     GIAATDIQHAAIAITAPNKCEGYAEGGQFCGVPTPYKVYFAAEFDEDAVKTGIWKNDK
                     LFENATFAEGENSGIYFTFDLKNRKQIQYKVGVSYVSVENAKENLRSENSAWNFEKIQ
                     NDAENKWNEYLSKIQVNGTNQDRITQFYTHLYHSFIHPNVCSDVNNEYMGADFKVHKT
                     RSKHYTSFSNWDTYRTQIQLLSILDPQVASDIVISHQLFAEEAGEAYPRWVLANIETG
                     IMQGDPTCILISNAFAFGARNYNPKPVFRLMQRNAEIPGLKCQNVEVRPGLKQYLEKG
                     YYNASIQLEYTSADFAIGQFALRAVGDEFSSWKFFETARSWKNLFNPETSWLQSRNPD
                     GSWKPLTEDFRESTYKNYFWMVPYNLHGLIELIGGKKDAEKRLDELFVRLDAGYNDPW
                     FAAGNEPSFHIPWIYNWVGCPYKTQLVVNRIINEQYSSANDGLPGNDDLGTMGAWYVF
                     SCIGLYPEIPGIGGFAINTPIFESIKIKLKNGDLIINGGSEKNIYIKSLKINDKVYDK
                     TWIDWNDLSNGAILNFKTTSKPNLTWGTKMLPPSFP"
     gene            14159..15295
                     /locus_tag="PLR72_00055"
     CDS             14159..15295
                     /locus_tag="PLR72_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOK35641.1"
                     /translation="MKQLKIKSIVFGIFVFLFTSCNISRNEYQPSFPLGEFIRPTGIN
                     PVLSPDTTSVFFCPMNQKLVHWEESDVFNPGAVVKDGKIFILYRAEDNSSVGIASRVS
                     RIGLAKTSDGFTMKKKPAPVVFPDNDNNKLYEWPGGCEDPRVSVTEDGTFVLFYTSWD
                     RKVARLCVATSKDLIHWEKHGPVFANAYNGRFLNHWSKASSILTTLKNGKLVIKKIDG
                     KYFMYWGEYVIYGATSDDLKNWTPVLDEKNELKVLMSPRKGYFDSDLTECGPPCIWTK
                     NGIILLYNGKNKQGEGDDNYVTNAYCAGQALFDANDPTKLIKRLDKPFLVPQEDFEKS
                     GQYPFGTVFIEGLVYHNQKWYLYYGCADSRVSVTIYDPFSKNKK"
     gene            15408..17276
                     /locus_tag="PLR72_00060"
     CDS             15408..17276
                     /locus_tag="PLR72_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOK35642.1"
                     /translation="MRNKVLIFFIPLCLLLGVSCSEKGFLDETVTTDLNRDVVFSDST
                     YTAGFLNEIYSQIGFDTDPDRFSTIAFGWKTPFGGLQTACDEARFKRSSTITTDVMFA
                     TGTVNPILVSKDAWQNCYANIRRVNVFLKYVEGSPIVDRIKNQYKAEARFLRAWYYSL
                     LLRHYGGVPLIGDTIYNINDDIKMTRDSYEDCVNYIVSECDDIINSNVLPRRRTGRDY
                     GRITEAACRALKSRVLLYAASPLYNGIEDKYAPTEEYKKLLGYPIYDKERWRIAYEAA
                     RDVIAMNEYKLFVWHQTASANDDISIPEIGWGFYAVTSMASDFANLTVNKDGEVFKDG
                     AFCEIILDKTAAKGNARERLFGPPTSGGGGEGGYIYKELADAFPMIDGKPIGQSKYTY
                     DVFNPNVNRDPRFANSVLYDGGYFCTGVSAYTPIRISQGVGATLDAVHVGTPTGFYVK
                     KTVSRATCGNSFIGVPQSRPLIRYAEILLNYAEAANEYYGPDYNDQYTSVYDALKAIR
                     ARGGIEPGDDGMYGLKPNMTQDEMREAIRLERRIELAFEGHRFFDVRRWKIAEETDNK
                     VMHGLEITVNNDGSKSWQEFEVAKHTFRPAMYFWPIPYDEVTKSNDLLQNPFYSAE"
     gene            17303..17755
                     /locus_tag="PLR72_00065"
     CDS             17303..17755
                     /locus_tag="PLR72_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5004 domain-containing protein"
                     /protein_id="HOK35643.1"
                     /translation="MKSKILKTVTLLSFFTFLISCDAFTDSEFNDYKESVKDLSGVWQ
                     LKTVSRNGIDISSTMNFSQFHLNLNPNGSYTLENYLPFAVKDNGEWRVDDPQYPYNLI
                     LRENNSSEDITIGLKYPIVNGKRIISITLSPGCQSNTYVYVFEKIDNN"
     gene            17769..18842
                     /locus_tag="PLR72_00070"
     CDS             17769..18842
                     /locus_tag="PLR72_00070"
                     /inference="COORDINATES: protein motif:HMM:NF027652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4961 domain-containing protein"
                     /protein_id="HOK35644.1"
                     /translation="MKNIFKNKKNTRLIGLFLTVGFVLFLIGCVFIDSVSILQIQEDG
                     TQAPRTKAGSVATFILTGHINCQEDHGDVQFIFSFLAPKSWNVRQNATVTYTTTLATN
                     PEEELKMSVVPESSLPKNGGGRTWGEALMQEYGVGPNVLSDMEWVTFATNDKWAIYNG
                     DKPNYTIYLRTNVGYQNLKAYLGFFVNHTDDGMSTSADHKKVKFSDTPFEVYGGKGLM
                     IDYSTDHFNKVQPLASLQDDYVTFSFNGGVYQNDLISCSEIYFEGIAYDENGEIISEV
                     NEKTDKTLLHRESMFSQVYSLTMWPASFFNVPQGKEIKKIDYVFTNKDRSIIITQSDD
                     DFAINHIPINSEKEPFVFELLCD"
     gene            18887..22006
                     /locus_tag="PLR72_00075"
     CDS             18887..22006
                     /locus_tag="PLR72_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOK35645.1"
                     /translation="MRNNKCQYLILFMLIWLVMGIQSVCVKAQDNSSKSSLTNIVLDP
                     YGNPVSNVEIGIKGSDIKTFTKINGTFDLDYKVGDILTYSHKNFMYSESKVSKKDVKE
                     GIQIHLQEKSIKNPSNVPEPYGEIIDKNSYLGSASTIYTDQLTKTMGSTVIPAMVGRI
                     SGLNIIQTRGARLHYTSANYNADLAGLIPVFGRRVYSDNSEFSLNARGQAPIVIVDGI
                     QREFYSLDPEAIESISLQKDALSSMFLGMQSSRGALIITTKKPTQGAMNISFTGRYGI
                     TTPLQLPKPLNSYQYTYLLNEALTNDGKLPIYSYEDFEKYRSGNSPYTHPDVNWFDEL
                     LNNNASTQSYNLNVSGGNNVAQYYVSLGYMNEDGLFITSSKNPYNTNLNYQRYLITSK
                     INVKVTRDFTTNIMLVGRIEDGNQPGGNGSGYADLLNTIFTTPNSAYPITNPDGSWGG
                     NISFTNNLMSQTINSGYIKDNARDILGTVNLNYDFDKLIKGLSIQAIGSITTQSRSAT
                     FRTKRSQVFAYKLDSEGNPVYTMYSTPSPQINSFSSVGNYQDMYGQLAINYDRKFGLH
                     GIKASVKGDTRTILNNYDLPEIPSNVMANLSYDFNNKYFVQSTLTESYYNRYATGKRW
                     GTFYAFGLGWDLSKEAFMKSVTWIDQFKLRGIYGLTGNGITNSGYYIWSQTYSYHGAA
                     WYPLGTSQSSGFFTTENQPIANPNITWEKAHKTNVGLDLSLFNSKIQFTADYYNDNYF
                     DLLQSRGKSIELIGASYPDENIGKARWYGGELSLTYQDNISKFNYYITGNWNIVQSKI
                     LFMDEQEQPYNYLYQTGKPVGAIFGLVTNGFLSADDIANGYPVIQGFKNIQPGDVKYE
                     DKNGDDVIDEFDRTVIGGNKPLSFFGIDLGVQYNGFEFSMMWQGVYNRDIYLSDRNFT
                     EGFLQINQHYGQAYEHMLNRWIPENAENATLPRLSAGSNNYNYGNGWGTSLWLKSGNF
                     IRLKNINIAYNLPESFTRSYLGNLRVKIFATGQNLFTMSATNLVDPEVSFTNYPIQRN
                     INFGVNVKF"
     gene            22014..23792
                     /locus_tag="PLR72_00080"
     CDS             22014..23792
                     /locus_tag="PLR72_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOK35646.1"
                     /translation="MKSKQNIWMIPIVGIVLLFFVACTDSYESFPVEQFTKDYVFSKT
                     DSMGVQARNFLNSLYGMLDNGHNGVGGDYLDAASDDAISIKQNEPDVYKLAVGQYSAN
                     NRISSTMGWGKYYEAIRKANIIIKNIDAVPFKLTFVNINGEVKPLNYTIKAEARFLRA
                     YFYFELVKRYGGVPLLGDSIYNLEDNMEIPRNSFADCIDYMVRELDTIKYDLRSVTMP
                     DPSVYAHAATIEAAMALKSRILLYAASPLFNEKPITTDNNAQKLLIGYESYDIERWKK
                     AADAAKYFIDTFGPTGKKTVDLAKNQMGVFLSFYSTTANPELIFFRQGGMDKSVETNN
                     GPLGFSGNSLGNGRTNPTQNLVDAFLMKDGKPRGESTLYPYDPQKPYDNRDPRLGLTI
                     LYNGANWLGRVLETYRGGANNPTGTGEYTKTSYYMRKFMGNFANATEYSNNYHHWVIL
                     RYTEILLNYAEALNEYLPAPSQDVYNAIISLRKRAGIPAGSDKLYGLKANMTKEEMRK
                     VIQNERRIEMAFEEQRYWDIRRWRIAEDIFKTPLKGMNIIKTLSGLNYSEVDVLTANF
                     EEKQYLYPIPYSEVIKNRNMVQNPKW"
     gene            23799..26879
                     /locus_tag="PLR72_00085"
     CDS             23799..26879
                     /locus_tag="PLR72_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK35647.1"
                     /translation="MKKLIITVIFLYACIIAFSQTFLLKGVVTSTDGETLIGVNVIKS
                     GTTIGTITDVDGKFEIQVKAGDKLKFSYVGYKAQEIIVKPNQQYINIQLAPDQFTNLD
                     EIVVIGYGKAKKLTMTGAVSAIPMREIRLVPTSSVQNALSGKLPGFFSVQRSGQPGKD
                     ASDFFIRGVSSLNDAGNQPLIIVDDVQYSYDQLQQININEIESISILKDASTTAIYGI
                     KGANGVLVVKTRRGEQGAPQINVRMESGIQTPVRKPKFLNSYETAKLVNEAYQNDGLI
                     SQIPFSEDDLEAFRTGSDPYGHPDVNWYNEIFKSIASQNNVNVDISGGSKNLKYFLST
                     GYFLQDGLVKNFNSGQNDVNTNYFYRRFNYRTNLDFDVSNNLTMRLDFSTRFMNINEP
                     SRLNATGEIYNFEKMTPYAAPVLNPNGSFTYLDTKGFLPTLNARLANEGYKRTKRNDI
                     NVLYGIIYKMDWLTKGLSGNFRLAYSSIDENYRSVSRASDGYPTYRYHPETDTYTINP
                     NQVYAYSPYGVTSGVGQAQNDLNIQASINYARVFNKDHDVSAMLLYNRESQTNEAAAS
                     VPSNFEGFTSTLGYKYKNKYLIDFNLAYNGSDRFGKNNRFGFFPALGVGYTISEEDFF
                     KNKFNNIQMLKIRTSYGLVGSDIAPGGRYLYRQVYQSGNGYYFGENPQYQASYKEGDL
                     GNENVTWEKAKKFDIGLDLVAFKNLSFTIDYFHDYRFDQLVTRYDIPLILGIGVSPLN
                     VAKTVNQGFDGSLGYQTKINQVNFLTNFVFSYAKNKVIYKAEAQQRYPWLAQTGKPIN
                     QPFGYKFIGFYTPEDIELINNNDPAAPAVPNTDTPVQAGDLKYKDLNNDRVIDDYDKC
                     AIGKPNLPTTSIGWTIGGNWKGFSLSILFQGSFDYSFSVVGTGIESFKSQFQPVHLKR
                     WTLERYQNGEDIQFPRLTTIPSTVNSAEGYMSDFWLIDAWYIRMKTVDFAYQLPKKTL
                     PKFINTARFYLTAYNLFTFTNYKKYQQDPEIQTNTAGDAYMNQRVVNLGVQVTF"
     gene            27002..28390
                     /locus_tag="PLR72_00090"
     CDS             27002..28390
                     /locus_tag="PLR72_00090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOK35648.1"
                     /translation="MKKLVILFFIIVCSGVNLFSQPVSKFKDGDRVVFLGNSITDGGH
                     YHSFIWLFYMTRFPNMDLLIMNAGIGGDTAYDMYKRLDDDVFCKRPTVLVVTFGMNDS
                     GYFEYNSDKAKEFGEEKYQTCYKNFKMLEKRLLELPNTRIVMMGSSPYDENVKINGNI
                     PFKGKNAVMQRIVEFQKQAAQEHNWEFLDLNQPMTAINQKFQQTDSTFTLCGLDRIHP
                     DNDGHMVMAYLFLKEQGFAGNKVADIEINASKGSIIKSTNCGISNLKKDGNKISFDYL
                     AYSLPYPLDTMPRGWNSKKSQAQAIKYVPFIDEMNQEILKITGLKGNYKLMIDDNEIG
                     TWSSDDFAKGINLASETKTPQYQQALAVMYLNEYRWEIERNFREYAWIQFDFFQQKGL
                     LFADNQEAVNVLDKEIKKTNDIWLKMHRDNYAQMMHKSVREAREEEMKLLISKIYEIN
                     KPVTRKITLKKI"
     gene            28583..29497
                     /locus_tag="PLR72_00095"
     CDS             28583..29497
                     /locus_tag="PLR72_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK35649.1"
                     /translation="MKKIFIFLLAIFAASVTYAGTWVIGVNQVDRFLKAIEKGTGDEP
                     PTVPMKVDPVTGENTINTDYDVWKDFPGITFTSETIVPTYLADQADADHHWYGIKEIN
                     YANKGYKQKEDVAIEFVQPSTVEWDYSGVPEGYSSLSNYFDRNDLSWLENIDLSGNDL
                     TNILIDGGPYNAMPLKTVNLSNNPNLTTLSIVNCTNLEEVNLLGTGLTPEAFEIVKEG
                     ILESSPSANILYASNEVKSIKIDDPVIVVQGGNIVIKNKKVNDQVIIFDLSGRKIIET
                     SNDVINANSIGKGVYMVKINNFVKKICL"
     gene            29744..32824
                     /locus_tag="PLR72_00100"
     CDS             29744..32824
                     /locus_tag="PLR72_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009039052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOK35650.1"
                     /translation="MKKLNLLFISLFVFLFNDIQAQTSIVSLNSSNPNIIWEIKPQAD
                     VNFSGNQISIPGFQFTDYVKGIVPGVVFTSYVAAGKEKDPNFSDNIYKVDEIFYNRPF
                     WYRTEFEIPTSYKKGQRVWLHFNGTNRFADFYFNGTKISGTPTSIKDVSGHMLRSKFD
                     VTDLINVTGKNAVAVLIWDADQKKRRDSKEAFGVASSPTYLAAAGWDWMPYVPGRLAG
                     ITGNVYLKITGDVIMEDPWVRSDLPALDVAEISIMPNIKNVSSTNQDVTLNGLIQPGN
                     ISFTKNITLPKGKTTNITINKNDFSQLVINNPKLWWPNGYGEPNLYTCNLKCIVDGKI
                     SDEKEITFGIRKYEYKMINNKVGWPVLNLFINGQRLFVKGGNWGMSEYLLRCHGDEYE
                     TKISLHKDLNYNMIRCWTGCVTDDEFYDYCDKYGIMVWDDFWLYVAFNDVAQPEAFKA
                     NALDKVRRLRNHPCIAVWCGANETHPKPELDNYLREMIAKEDNNDRLYKSCSNQDGLS
                     GSGWWGNQPPKHHFETSGSNLAWNDPPYPYGSDYGYGFRSEIGTATFPVYESIIKFMP
                     QDQLWPLPTDEQLRNDDNTIYNKHFFGKEASNANPVNYKRSVNTEFGESANLQEFCEK
                     AQLLNIEVMKGMYEAWNDKMWNDASGLLIWMSQSAYPSFVWQTYDYYYDATGAYWGAK
                     KACEPQHIQWNCSSNSVKVINTTSKALTNVKAMVNVYDINGKEIGKYRKSAVLNVNPS
                     NINEAFVINFNTSNLALGKKAFASSSTKNMDAALITDGASGSRWESEYNDPQWIYVDL
                     GKKQKIESVVLKWEAAHAKEYELQVSNDANNWTTIYKQQDSKGGTETIKIKPVSAQFV
                     KLNCISRATIFGYSLFELEVYGKMQKQQELSPLQFVKLKLNDNQGNLISENFYWRNGI
                     HDEDFTALNNMKEATISCKLIDKRQENNKEKIKLLIKNDSKIIAFANRIRLVNSVTKE
                     RVLPVMMNENYFSLLPGEEKIITIEADSQLLKGGADILLKQFRKEERNILKIID"
     gene            32915..37099
                     /locus_tag="PLR72_00105"
     CDS             32915..37099
                     /locus_tag="PLR72_00105"
                     /inference="COORDINATES: protein motif:HMM:NF025563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HOK35651.1"
                     /translation="MKKNILFLFCLLAFNILKLSAGTWVIGVNQVDRFLKAIEKGGGE
                     EPPAIPLKDDGTINTNYYGVWEIFPGITCTSEDIVPFYMEGQGSDHHWRGIKKINYAN
                     KGYKQKSGTVISFVQPSLNASDYPEIPTGYTSLSNYWDRNDLSWLEIIDFSGNDFENI
                     EVDGHNIMPLTEISLANNPHLKSLIIKDCPSLTNVNITGCNLTFSFISNLRNIINLSA
                     NAEFLYEYQGEITSYYAAVDLTELLEKNGEGSRIKSWSIQPIKNEGNIYTFDSSHVGE
                     VVRVSLENSDYPGIIISYDINLTSAIPSTISINATYCKVKIQNLTTGNNNFSIGDEAL
                     ITVYPYSCSVLESFTIDGEEVTLDSKYQYYFIVNKTFHQITSIFKKDDMPTGQTSNYL
                     RNGSFEYGLNLDWEYNIGEGTSAEFTLERGSNVIDGNVALRVDVNSLKTPNSVSAKTH
                     VAVGCDSLYLLHFWAKGPEESKLYVQIDGAEQNGIQYELHEGATAYFYPIKIDKTKAN
                     EGLTITFYFQNDFTKVKVADPCSVTTYAGATYYLDDLELVDQFNNLTYDVYNTYLWNY
                     NHRPNNEGKMWVAGDNSVSYDLPDGRKMWFFNDSFYGVLHPESNRLVDVGRFVRNAVV
                     VQNIDGTLTTYPVTNQGGQWTYFRVPDDAVIYNDDGSVKNIFWVGDALMEDNQIKVYL
                     IEVYGEGRSYLGKFSYPGLEFLGVEKQENFCRKYETFFVEDNKIYLYKTEDVGTWGRY
                     MHVARADLGDLSGKKGTWEFWNGTEWSKDSTQTARIYDMASDGVIRLESGNYAHVSMP
                     VLSPEVYVSFAPAPQGPWTNKKLIAVGDKSANYWYYMPNFNGKLPNGKYAISFSANYN
                     YCLFFCKDCETSSFVDKYWYRPRFMQVDLLALSPYTKNHKDCAGVENGNAYYDTCGHC
                     VGGTTSIEPCLIGVAKLYANANYSGVGIGLNAGEYTSNDLSNLGFEVEALSSFKLDQG
                     YVIELYSEDNFQGEMKLFESSVKDLSEKNFDNQTKSLIIRRKGIENLSGIYAIQNKQS
                     ELYLDVEGSNPANNASIIQSLYKNSDFQKFKLNNLGKGFYALENMGSEKVLNIANQSK
                     EERAYVEQWDGKEIDITTQGGEISAQYSDSPAGEDISKIIDKNPATKYLTFHSKAWVQ
                     FKCSQPWIVIRYTLTSANDAPVRDPRSWTLSGSNDGIYWTILDTQSGISFTKRFEEKS
                     FPINNEISYLYYKIDMTCSSGSILQVAELKLFAKTNISDGDYFNSQKFVIQDGGNDFV
                     KFINKNSDLMLEIVDGFTNEGEKVWQNYDYGQWGGFWKLVDPTSISNEVGTLLSNKDI
                     LIYPNPASAYLIIESNQSEIITRIIVADLSGRIMQDKTYNNSKIVLSLSDLQKGLYLL
                     KINTDRSSYGHIIIRN"
     gene            37130..40390
                     /locus_tag="PLR72_00110"
     CDS             37130..40390
                     /locus_tag="PLR72_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOK35652.1"
                     /translation="MFKKIIVFLLATTFSLQLYSQYIYSLVSSDKILWKVFPVDMVTA
                     SISDLSTPEFDVSSWVIAKVPGTYFVDYVNAGLEADPNYGDNIYLVDEKKYNKEFWYR
                     TEFEVPADFVKDKLWLNFKGVNKEGTFFLNGELLGSVKGHMLRSVYDITNQVKVGKNV
                     LLVKTNVPDVRRERQPTGVFDTWANFAMPTYMGSASWDWMPYVPGLNCGITGNVYLST
                     TGMVKLDDVWVRSDLPDDSHANLSIQATINNTCNQSVTGILNGTIMPGNIHFSKTVVI
                     EANQSLLVKINQEEYAQLIINNPLLWWPIGYGNQNLYTLSLEFKVNNEVSDAQAVIFG
                     IRKYEYKNENGAFTLYINNSKVFVRGGNWGMSEYLLRCKGEDYDTRLKLHADMNLNMI
                     RCWTGCVTDDEFYDYCDKYGIMVWDDFWFHGSLFPPSEDIFMSNAIDKIKRLRNHPCI
                     AVWCGANEGIPAGNLDQLLKNAVADYDGNDRHYQSCSNSGGGLSGSGWWLNMHPTNYF
                     TGPGSGGGDWNGSGTWAFRSEIGMATFTTFESFQEFMPVDSWWPPKNEMWNKHFFGSQ
                     AQNAGCDTYFNTLSDNYGTSSEIDEFCEKSQYLNLEVMKAIYEGWNDNMWNNATGVLI
                     WMSQSAYPSFVWQTYDYYYDATGSYWGAKKACEPLHIQWNCANNSVKVINTTLNNYAQ
                     LKAKASVYNLKGEEYLPLCMEKLVESKSNTATECFVLTEKNSINLALFKKAYASVNEN
                     DGEGVPSRAVDGNLSTRWGSGYQNNAWFYVDMESTILINKVKIYWEYAYGKKFQIQVS
                     NDANNWITVYTQNNGTGGVETISFDPVLARYVKFQGIERGTMWGYSFYEFEVYAPSSN
                     DENNENSVELTKLNFIKLQLFDENNNLLSENFYWRNMDEYNYQDLNTLPKADVTIIET
                     HRKENGKYFVSCVVKNNSSTIAFGNRLKVVNKQTGKRILPIFLNDNYFTLMPNESKNI
                     EIEFSETLIGKDNFEVVLKQYGNYDSTPVQSNVEKESMISSDVLPVYPNPASQVITLG
                     NPATRISEVKIYNIFGSLVHSASYCSIIDISKLSPGIYLVSAIIDNKLYTSRFIKD"
     gene            40411..41820
                     /locus_tag="PLR72_00115"
     CDS             40411..41820
                     /locus_tag="PLR72_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOK35653.1"
                     /translation="MKRLICTLIGSVIFCGFYSYSAVQELSKEATISLLIASPNPDQL
                     VSSYGHSLLRVKDPKLNIDYVFNYGTFDRTLSGFETFMGIATGKLKYEIWVIPYTEYY
                     ETTLKENRKLVEYTFNFSAEEKNTIWHNLISIVKNKNQYLFDFFSQNCTTFVRDLITY
                     NLGKQILLPSDLGQKTYHDINIKYTKNKLWYVFLVDLISGMNLDKKAPSYESLYEPKE
                     LVNAWSKSFILEDNGEKKPIFASASLLINGKNLINETKTPFIISPLFFSLVLLVLVLS
                     LSIIEWETGNYYRWFDILIFGFTGFLGLLFYLFKFACPRWYFLMDWKMFWLHPFHLLA
                     VFFLIKSLNKPLKIYHYINSAILAFLIVGVFFLPQHFNPAIILLIICLFIRSIFFVLR
                     ERIPNKKKTIRKLFILKSSSHNQSSVKLLKSKEFLENIKFPLLSNKTAFCFNMSFVSN
                     STNFLSYKRAKIDFLSGVI"
     gene            complement(42066..43694)
                     /locus_tag="PLR72_00120"
     CDS             complement(42066..43694)
                     /locus_tag="PLR72_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HOK35654.1"
                     /translation="MKELRENQQPYVSQVWSPDLGNGKYKNPVIYADYSDPDVCRVGN
                     DYYMVSSSFANTPGLPILHSNDLVNWTIVGHAIQNLTPAEKYNKMQHGNGVWAPSIRY
                     HDNQFYIYFGDPDEGIYMTRAKDIKGSWSSLYLVKKGKGLIDPCPLWDEDGRAYVVHG
                     FAGSRAGMKSVLGIFEMTPDGTQALSESRLIFDGHPANPTVEGPKFYKRNGYYYIFAP
                     AGGVKPGWQLALRSKNIYGPYESKIVLSQGETAINGPHQGAWINTPDGKEDWFIHFQD
                     KYAYGRVVWLEPLQWINDWPVIGEDKDGDGCGNPVLTWKKPNIGKVYSPENPMESDEF
                     NTPMLGLQWQWQANNNPLWYRLDSESGNLRLFAWQPDENGKNLWDAPNLLLQKFPAPN
                     FKATTKLVFSPSKIGESAGLVVMGQDYAAITVDSTQNGLFIKQTICKEASKGNAEVVT
                     DSVLLKKNIPLYFRVEIRQTEEKNREEILQPLANCQFSYSWDGKKYVTIGKSFIAKEG
                     LWIGAKVGIFCKRPHFSNDAGYVDVDWFRVESVK"
     gene            complement(43752..44378)
                     /locus_tag="PLR72_00125"
                     /pseudo
     CDS             complement(43752..44378)
                     /locus_tag="PLR72_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
BASE COUNT        15437 a         6646 c         8407 g        14523 t
ORIGIN      
        1 gcgaagcatt ggagaaggat attgatgaac tgttcgagtt gatcatgaat tgtcctaata
       61 cagcagaatt agtttcaaga atgcttgctc ctccactcca ggaagcagtt tgacgaaaat
      121 gaaatcaata atgtattgat aatcagacgg gaaataatga aattgcactt cattatttcc
      181 tgtctttcaa atcattaaaa cgaagtgcga aactttagtg tttttacaat taatcgagct
      241 caatggtttt attttgcaaa tctttgttca tttccaaccc tacctagtat tctcattttc
      301 ttgtcctgaa tattctgata aatctcttca attccaggaa aataaaaata actgaccata
      361 aaatcgagac tgcagactac agttttgttg gataaaattc cctgagaatg atgctggaaa
      421 gtatttttcc ccttttgttg gtaataaagt gttcgctcat agttatgtta gaaatttaaa
      481 gatcagcttt ttagataaac gtataaaaaa ttaaaaaaaa ccctacggga aatcaatttt
      541 taatagtttg taagctactg aatatcagca gtaaattcta agatgttctg tggaatttac
      601 tgctggattg ttaaaagaca ggtgtatgaa aaagatgttt tcttctaaaa ttacaattaa
      661 ttatttgaat atcaaaattt tagtttataa gagtcatatt tattataaaa aactttttag
      721 catgaaattt ttaataataa ttaattacga taagaccttt gagctaagtt catttaaagg
      781 tactaaatta catcaatttt gatatcttat tttgatctct tggtgtgttt tttaaaaaat
      841 tattatgtaa atttgtaata cgaaaattat ttaactcgtc atcattttta tagttatgtg
      901 aataacactt tctccttttt tcgggacgag tgaaaaatag gatataatga agaacgtttt
      961 tttgatatta ttatttatta tttgtccaat cactgttttt agtacaatta ataagactga
     1021 ctccttgttg aaagtgttag ataaagttat atctgaacgt gacagctact taaaaataaa
     1081 agaggaaaaa attttagagc tgaagaaaaa gaagcaacaa tcaaaaactc ttgaagacca
     1141 aatcaatata aatcgagaaa ttatagacga atataatagc tttatttgtg actcagcaga
     1201 attttatata aaccaaaatt tactgatagc taaacaatta aataacaaag aacttatact
     1261 tgaaaattac tttaggctat cttatattta ttccttatct ggtcttttcc tcgaagcagc
     1321 ggagatattc aactcaatca attatgaatc tttatctaca ggttataaag catggtattg
     1381 ttggaaccga atacgatttt atgaaaattt aattaattat accgctgata caaaattttc
     1441 aaaacaatat gaaaaggaaa aggaagagta cagggaaaaa gtgatgggtt tgctctacaa
     1501 tcagtcagat gtatataaaa aggaactggc ttgcaaattg gaaacagaag gacgatacga
     1561 agaagcaata aatttgttga ctcctatttt ttataaacag cagcccgaaa cacatgatta
     1621 tgcaatggca gccatgaatt tggcaaaagt atataagctg gcaaaaaaat acgatgaaga
     1681 agagtactat ttaatattgg ctgcaacaac agatataaaa ttagctgtaa aagaaaacga
     1741 agcattgtta tcattagcca ttaatttgta caatagggaa gatgtaaata gagcctattc
     1801 gtatgtacga gtggctttgg atgatgctct tttttataat gctagatata aaaattctgt
     1861 gatcgggcga gttcagccaa tcatagaaga cacttatctt caaaaaatta agtatcaaca
     1921 aaagaacctc aggctttatt caatgctcac tagtgtattc attgtcattt tagcattttt
     1981 cttatttttc atttataaac aaaataaagc tctttctaaa gccaggaaaa aacttcgtga
     2041 ggtaaacgaa gaccttttac acctgaatca aaaattgaat gaagctaata ttataaagga
     2101 aagatatctt ggctatttta tgaaccaatg ttccatttat atcaataaac tttataagta
     2161 tagaaaagaa gtaaatctta aaataaaaac aggtcaaatt aatagcttat acaaatcatc
     2221 aacttcagag ttggaaaaag aaattgaaga acttcgtaca aattttgata aggcgttttt
     2281 gaaattatac ccaaactttg tgtcggaatt taattcctta ttaaaaccag aggaaagata
     2341 cgaacttgaa gatggtcaat taaatacaga actcaggatt tttgcgctca taaaattagg
     2401 aattagcgat gtaagtcaga ttgcagattt tcttcattat tcatcgcaaa ctgtttataa
     2461 ttataaaagt aaagttaaaa gtaaatctgt catcgaaagc gaccaatttg aagaagaagt
     2521 taaaaaaata ggtaaactgc aatagtattt tcattcaaat atatttacta aatcattttc
     2581 ctgtatattt ataatatatt gattaaaagc cttttaactc agctttgatt ttaaaattat
     2641 ctactttaga gcctactaac gatgttaata ggctttttct ttatttatat ttgtgataca
     2701 actttttgga aattatctat ccagggagta taaataatta attaattaac caaaataata
     2761 atactatgaa actttattac ttcctattat cgtttattac aagcttattt ttagctgact
     2821 gttcccaaac atcctttctg tttttttaaa gtttttcatc attaaaccat aaaattatgg
     2881 agagggattt ctcaaatata tatatgacat tttattcacc attggtgagg tttgcaatgg
     2941 tatttgtcat ttataaagaa gaggcagaaa atatagttca agatttattt gtagggcttt
     3001 gggaaaagaa ggaatcttta aattgtgttg aaaaccttaa agcatatatg tttaccttgg
     3061 caaagaataa atgccttgat tatctaaagc ataagttagt aaaagaaaat tttattgcta
     3121 aagcccaatc cttttacgaa caggaaattg ctttaaaagc acaatctttg gaaagctttg
     3181 atgttgaatt ggcttcggaa gaaaaagtag aaaaaatagt ttcagaggca attgaatcgc
     3241 tacctcctaa atgtcgggag atatttatat tgagtcgtta tgaaggacta aaatataagg
     3301 aaatttcaga aagattaaac atttcagtaa atacggtgga aacccaaatg cgtattgcct
     3361 ttaagaaatt gagaaacagc attaattatt tattaaatta gaaaattagt tatcatttta
     3421 ttttttcttt tttaatggaa ctcaggttag aaatcgttct taattaaaaa agaacgaatg
     3481 ataaacttag ccataaaata tcttaatgga gaattaactt cagaagaaaa ggaacgtttt
     3541 tttgtattag tagcgaaaga tgaaacttta aaaaatgatc ttataaaata tcatcattta
     3601 cttgcttata ttagtttact ctttagaaag gaagataatg ccgatactga aagaaggttt
     3661 ttggatttgt tgaatgaaat tgaaaggaaa aaaagaaaag agaaggatgg ttaaggttaa
     3721 agtaagaata acttggacac tgttggtttt aagtatattt cttaatgcgg agaatgtata
     3781 ttttgttgat ggatatcatg gtggagtata cggacattat ccattggaat gggaaactcg
     3841 atttataata gataatttct ataaatatcc tgattggaga atatgtttgg agatagaacc
     3901 tgaaacctgg gacagtgtaa aagttagaac accacaggat tacacacaat tcaagcaaat
     3961 tctttccgat gaaaggatag aatttactaa tccagcttat gctcagccat attgttataa
     4021 tatttccgga gaaagtatca ttcgccagtt tcaatatggc atgagaaaaa ttaatcaaca
     4081 ttttcctgat gtaacatttt ccacttattc ttcagaggaa ccttgtttta cgagctgttt
     4141 gcctcaattg ttaaagtcgt ttggttttca atatgccgtt cttaaatgtc caaatacttg
     4201 ttggggcggt tatacaagag cttacgggaa agatttggta aattgggttg ggccggatac
     4261 aacttcaatt ttagccgttc cgcgttacca atgcgaatct tttgaaaaaa attccacatg
     4321 gcaaacaata gcttggaata attcaacctc ctatataaat acttgtttgg aagccgatat
     4381 aaaaaatccg gtgggtatgt gttttcagga tgccggctgg aaaaacggcc cgtggttaaa
     4441 ttctataaaa aatgccgatc ccaattccat ttatgtaact tggaaagaat attttgaaaa
     4501 aatttctgat ggaaaatcaa ccgacaattg gcaattttct caggaagatg ttttggtcag
     4561 tttaatgtgg ggaagtcagg ttttgcaaaa aatagctcaa caggttcgca atgctgaaaa
     4621 taagataatt attgccgaaa aaatgggctc tctggcttat cttaaaaaca attttcatta
     4681 caaacaggac agtattgatg aagcttggcg aacattgatg ttgtctcagc atcacgattc
     4741 gtggattgtt ccctacaatc gacttcaaaa aaatacgaca tgggcagata aaattgctca
     4801 atggacagaa actaccgata aaattgccga cagtgttgtt aaagaggcag ttgaaagttt
     4861 tcaaggtaac aataaatcag taaatgacga ttattctatt agagttttca atacgttggg
     4921 ttttaatcgc aatgaaattg tttctattct gttgcccgaa acattaaaaa acaaaaaagt
     4981 aatcctttta gacactgaaa atcaagtcct tccaagttca gtggaagaag gacaaaaaat
     5041 ttcctttctt gctcaagtac ctgcatttgg atatgcaacc tataaaattc aaatgggaga
     5101 atcgaaaata aagaaggaaa agaaaacttt ttattcaaat aaaaatgaat gcattctgga
     5161 aaatgacaaa tacagaatcg ttttcgattt acaaaaagga ggcacaatta aaagtttgtt
     5221 tgccaaaaac aataataaag aatatgtaaa aaaaggcagt gagtttctgt ttggagaaat
     5281 gagaggcttt ttttatgaag aaaataaatt ccattcatca agggaaaatc cagcgaaagt
     5341 tactttaata aaagataatt cattagaaac aagtatttta attcaaggta aaattgcgtc
     5401 gcagccattt aagcaaatcg ttactgttaa taaagagcag gaacgtatcg atttcgatct
     5461 tgttataaat tggaagcaga atttgtgtat tggagaatac gctcagccaa ataattggca
     5521 agacaacaga agggcatttt acgatacgcg ttacatgtta agtgttcttt ttccggttga
     5581 tcttaattcg ccacaacttt ataaaaacgc tccctttgat gtttgcaaaa gtaaattgga
     5641 caatactttt tttaatcgtt gggacagcat aaaacataat atcatattga actggattga
     5701 tttttctgaa gtaaatggcg aaaacagtct ggcactttta accgatcata caacttccta
     5761 ttcttatggc actgattttc cgttggcttt aactattcag tactcgggaa aaggtttgtg
     5821 gggacgggat tataatataa ctcaacctac gcatgtaaaa tatgcgcttg ttcctcataa
     5881 atttcggtgg gataaatctt ctatatcaaa aaaaagttcg agttggaatg aaccacttat
     5941 tggcgtttgt tcttccgatt tcgattttga aaataaaagt tttatcgatt tgctgagcag
     6001 cggttacgaa ttaagttccg ttcagataat gaatgatgga atactggttc gtctttttaa
     6061 ttctgaagga gatggaagtg ctcagaaaat taaattgtgt ttccctttta aaagcattga
     6121 ggaaatggaa ttgaatggtg aaatgaaaaa tgcaaatggt tcaataaagg caattcttgg
     6181 taacgaaata caattatcaa ttcctcgctt tggtgtaaga agtttttata ttaagaatta
     6241 attttcattg aagtcccatt ttgactggtt cggatttttt cttcagcttt taatctttaa
     6301 aaaaaatatg attagaaaag agcacatgta taaatttttt tggagtaacg tttctgtttt
     6361 tttatttctg ttgttcactt cctgtggagt gaaaaattcc gataaggaaa aaattcggcc
     6421 tgtcgattat gtgaatccgt tcataggagc cagtacaagt ataggagatg caggtattta
     6481 tcatggactg ggaaaaactt ttcccggtgc tacaactccg tatggaatgg ttcaggtaaa
     6541 tccgaatacc ataacgggag gagataatgc acccggctac agctacgaac acaaaacgat
     6601 agaaggtttt gcttttactc aaatgagtgg cgttggttgg ttcggagatt ttggaaactt
     6661 tttggttatg ccaactacgg gtgaattgta taaaattgca ggaaaagaag atggtagtat
     6721 taaaggatat cgttcgcgat acgataaaaa atcagaaata gccagagcag gatattattc
     6781 ggtaaaatta tccgattaca aaattaaggt tgaatgtact gcaacaccac attgtggaat
     6841 attgaaattt atatttccgg aaaacaaaaa atctcgaatt caaattgatt tggctcacag
     6901 atccggtggt acgtctacgt atcagtatat cgaaattgtg gataaaaata caataaaagg
     6961 ctggatgaaa tgtcctcctt ccggcggcgg ttggggaaat ggcgaaggga aggccgatta
     7021 tacggtttat ttttatgcac aattcagtaa accactcgaa gaatatggct gttggagtgt
     7081 caatattccg gataactgga agcgaaaaaa agatgaagta gtaagcattc cttatttgaa
     7141 aagagtggct gaagcacctg tttttcgtaa gtttaaaaaa cttgaaggaa aacatttggg
     7201 atttttcacc gaatttccta ccaataaaaa tgaaattatt accttaaaaa cggggatttc
     7261 gtttgtcgat atggcgggcg ctgaaaaaaa ctttaatgca gaaataaaaa ataaatcttt
     7321 tgatcagata aaagcagatg cacaaaatct ttggaatgaa gaattaagca aaataacgat
     7381 ttctggcggt acggaagatg aaaaaaccgt attttatacc gcattgtatc acaccatgat
     7441 tgatccaaga atttatacgg atgtagatgg gcggtatgta ggtggagatt tgaaaattca
     7501 tgaaaaagat tcaactttta ccaaacgtac tattttcagc ggatgggatg tttttagaag
     7561 ccaatttcct cttcaaacca ttataaatcc tcaactggta agcgatgaaa ttaattcgtt
     7621 gactacgttg gcagaacaaa gcggaagaaa atattttgag cgttgggaaa tcgtaaatgc
     7681 gtattccggt tgtatgattg gaaatcctgc catttctgtg ttggccgatg cttatgtaaa
     7741 aggaataaaa tcctatgata ttaataaagc gtatgagtat gcgttgaatt cttctgcaaa
     7801 atttggcaac gacaagtttg gattcgcagc aactcctttt tgtatttccg aaacattgga
     7861 atacgcttat accgattggt gtattgccca attagcaggt aaattgggaa aaaccggtga
     7921 tgaaaaatta tttttaagta aatctcaagc atataaaaat atctttgacg cagacaaaag
     7981 ctggttccga ccaaaatatt caaatggctt gtggaaaaca tggcctaaag aaggtagatt
     8041 aaggcaagga tatggttgta ttgaatcgaa ttcctatcag cagggctggt ttatacccca
     8101 cgatgtgaat ggtatggtag aaataatggg agggaaagaa aaggttttaa aggatttgaa
     8161 ttcttttttt gaaaatacac cgtcaaattt gcggtggaat aattattaca atcatgcaaa
     8221 tgaaccggtt catctggttc cttttttatt taataggata gatgtaccct ggctaacaca
     8281 gaaatggact cgatttattt gtcggcaagg atatactaac aaagttgaag gtttagtagg
     8341 aaacgaagat gccggacaaa tgtctgcctg gtatgtattg gcttcatcag gtattcatcc
     8401 ttcttgtcct ggtaacacaa gacaagaaat aacgagtccc gttttcaaca agattgaatt
     8461 taagctagat ccaaaatatt tcaaaggaaa aaaatttacg attatagccc acaacaatag
     8521 tccgaaaaat atttatatac aaagagcaaa attaaacggc aaagagtaca atcgttgcta
     8581 tattgatttt gcagatattt cttcgggagg cactcttgag ttgtttatgg gggagaaacc
     8641 aaataaaagt tggggaataa actgaaacat tttgatgata agatgaaaag atgtttttat
     8701 gttatagttt tacttttttg ttttttattt gccagattcg attttattta tgctgatgga
     8761 atcggccgtc catggaatgc cctttggatt ggaccaaaaa acgataaagg aacagaatat
     8821 ggcgtttatt attttcgtaa aagcataaca ataaaagaaa agcttgaatc ttttaatatc
     8881 catgtctctg cggataatcg gtacaaattg tttgtcaacg gaaaggttgt ttccatgggc
     8941 ccggctcgtg gagataccta cttttggaat tatgaaacgg ttgatattgc cccctatttg
     9001 gtatcgggga ataattgcat tgcagcattg gtttggaatg aagctgaata tcgtcccgaa
     9061 gcgcaaatca gtatacgaac tgcttttatt ttgcaaggta attccgaaaa agaagagata
     9121 ttaaattcca acgaaacttg gaaatgtatt taggataagg gttatcaacc ggtacctgga
     9181 tatttttttg cagctactaa aggacaattg gtggatatgg caaaaacagt taaaggtgat
     9241 tggatttcag taagttatga tgacagaaat tggccaaatg ctgtaaaaat tttggaagga
     9301 aatcttaaag gaaagtccga tggtttggct tggcaattgg ttccttcttc tttaccgcaa
     9361 atggagttga cttatcaacg catacccaaa ttacgttggg caaaagggat gaacgctccc
     9421 ttgggttttc aaaaattaaa attgaacctc atttaggaac gacgactaaa gttggagggg
     9481 agatgcctca ttcaaatggc aaaatatcgg taaattatga gttgaagaag aaaaaatgga
     9541 taatccatgt tcatttacca caaaatacgc cgggaaaatt gatatggaaa ggtaaaaatt
     9601 atgaattaaa tcctggcgat aattcactgg taatttgaaa tgaaaaaata aaatatatta
     9661 gataaattaa aaaaatagag cgatgaaata tagattgtta gctttaattt cttgttcttt
     9721 cataatgtta agtactttaa aggctcaaca aaaagatctt gtaaagtatg tgaacacttt
     9781 acaaggaacc gattcaaatt ttggtctaag ttatggaaat acctatgcca ccactgcaat
     9841 gccatttgct atgcatactt gggctgcaca aaccggaaaa aatggagatg gctggaagta
     9901 tcagtattcg gcagacagga ttcgtggttt tcagcaagca catcaatgta gcccgtggat
     9961 gggagattac gctgtttatt ctttaatgcc ggaagttggg aaattggttg taaatcagga
    10021 tagcagagct tccaaattta gccacaaaaa cgaaatggcc aaaccccatt attacaaagt
    10081 aaaatttgac aacggaataa ttacagaaat agctcctacc atgcgtggtg tgcatttgcg
    10141 tttcacttat cccaaaaatg aagatgctta tttagtactg gatggttata ttgataaaag
    10201 tgaaattaat attgacccaa caaaaagaca aattaccggc tgggtgaata atcagcgttt
    10261 tgtaaataat gcttcaactt ttcgcagcta ttttgtaatt caattcgaca aacctttcga
    10321 agagtttgga atttgggaaa acaacaaaga tgaaattttt gcaggtaaaa cttccggaag
    10381 cggtaaaggt tatggtgctt atgtgaaatt taaaaaaggc gctgtagttc aggctaaagc
    10441 ggcttcatcc tatatcagtt acaaacaggc ttggattact ttcaatagag aattgggaaa
    10501 ggataaaaaa attgaaatga caatggaaaa aggagccaaa acatggaatg acttattgaa
    10561 tcgtgttctg gtggaaggcg gaacggaaga acaaatgaaa actttttact cttgcctgtt
    10621 cagagccaat cttttttctc gagaatttta tgaattggat gcaaacaacg aaccttacta
    10681 ttacagtccg tatgatggga aaattcataa aggatacatg tttaccgata atggtttttg
    10741 ggatactttc agatctcaat tcccgctaac aaacattttg catcccacta tgcagggaag
    10801 atacatgaat gcacttttgg atgctcagga gcaatgtggc tggcttccgt catggtcttt
    10861 tcccggagaa acgggaggta tgatagggaa tcattccatt tcattactta ctgatgcttg
    10921 ggcaaaagga atcaggactt ttgatccgga aagggcgatg aaagcttatg cccatgaatc
    10981 tatgaataaa ggtccgtggg gtggttcaaa tggacgtgct ggctggaaag aatattggca
    11041 gttgggcttt gtaacttatc ctgaatcaat gggttctact tcgcagacgc tggaatatgc
    11101 ttatgatgat ttttgtggtt atcagttggc aaagatgacc ggaaatgaat tctatgaaaa
    11161 catatttgca aaacaaatgt ataactataa aaatgttttt gatagcaaaa ccggttttat
    11221 gagaggacga gataaaaatg gaaaatggtt ggaacctttt aatccatatg aatggggagg
    11281 cccatattgc gaagcaaatg catggcacta tacatggtcg gtttttcacg atgtacaggg
    11341 actgattaat ttatttggta gtgatgaaaa atttaccacc aaaatggatt ctgtttttac
    11401 agttcccaac attattgttc ccggcactta tggaggcgtt attcatgaaa tgaaagaaat
    11461 gcaacttgcc aatatgggtc aatatgctca tggcaatcag cccattcagc acatgatatt
    11521 tctttatagt tatgccggac aaccttggaa aacacaatat tggtcgcgcc aaattatgga
    11581 tagactctat aattctacag agaaaggatt tccaggagat gaagaccagg gtggaatgtc
    11641 ctcttggtat gtattaaatg cattgggtat ttacagtgtt tgtccaggaa cagacgaata
    11701 tgtattggga agccctctgt ttcagaaaat taccattacc actgaagata ataaaaaatt
    11761 tgtcatagaa gccaataata attcaaagga aaatatttac attcaatctg tttcgctcca
    11821 tggcaaacct ctggaaaaga attatattat ttacagcgat atagttaatg gtggaattct
    11881 tcattttgaa atgggaaatc aacccaacaa gtcaagaggt atttcaaaac aggctgttcc
    11941 tttttcattg tcaaataata aattttaaac agatgaaagt taaagtatat tttttatgtt
    12001 tattaagttt aagtatctgc tcttcaatca gtagtagcca aaacttaaat attaattatg
    12061 ctaatagagt aaatactttg attggtacag gtggttttgg attgaagtca ggaaattgtt
    12121 atcccggtgc aacttatcca tttggaatgg tgcaatttac ccctacatat ttttcaaaaa
    12181 acttgggttt tgtaatcaat caattaaatg gtgcaggatg tgaacatatg ggcaattttc
    12241 ccacatttcc gttaaaaggc aagcttaaaa tatctcctga taatattttg aattctctga
    12301 taaatttatc taaagaagaa ggacatgctg gttattataa ggctgttgtt gaggaagaaa
    12361 tagtagcgga attaaccgtc acagaaagaa cagggatggc tcgatatact tatccatcaa
    12421 gtgaagaatt tggaacgata atcataggag caggcattgc tgcaacagat attcaacatg
    12481 ctgccattgc cattacggca cccaataaat gtgaaggtta tgccgaagga ggtcaatttt
    12541 gtggcgtccc aactccttat aaagtatatt ttgctgcgga atttgatgaa gatgccgtta
    12601 aaactggcat ttggaaaaac gacaaattat ttgagaatgc aacatttgct gaaggtgaaa
    12661 attcgggtat atattttact ttcgatttga agaatagaaa acaaattcaa tataaagttg
    12721 gcgtttccta tgtgtcagtt gaaaatgcaa aggaaaattt aaggagtgaa aacagtgcat
    12781 ggaattttga aaaaattcag aatgatgctg agaacaagtg gaatgaatat ttaagtaaaa
    12841 ttcaagttaa cggaacgaat caggatcgca ttacacaatt ttatactcat ttgtatcatt
    12901 catttattca tcccaatgtt tgcagcgatg tgaacaatga atatatggga gctgatttca
    12961 aagtacataa aactcgttca aaacattata cttcattcag caattgggat acgtaccgaa
    13021 cccaaattca attgctctca attttggatc ctcaagtggc ttcggatatt gttatttcac
    13081 atcaactctt cgcagaagaa gcgggagaag catacccacg ttgggtttta gcaaatatag
    13141 aaaccggaat tatgcaaggt gatccaactt gtatattaat ttccaatgct tttgcttttg
    13201 gagcaagaaa ttacaatcca aaaccggtct ttaggttgat gcaaagaaat gctgaaattc
    13261 cgggtttaaa atgtcaaaat gttgaagttc gtcccggttt gaaacaatat ttggaaaaag
    13321 gatattataa tgcttccatt cagctcgaat atacttctgc tgattttgca attgggcaat
    13381 ttgccttgcg ggcagttgga gatgagtttt cgagttggaa attttttgaa acagcacgct
    13441 cgtggaaaaa tctcttcaat cccgaaacaa gttggcttca atctcgcaat cccgatggtt
    13501 cttggaaacc attaacggag gattttagag aatctactta taaaaattat ttttggatgg
    13561 ttccttataa cttgcatgga ttgatagaac ttattggcgg gaaaaaagat gcagaaaaac
    13621 gtttggatga actttttgta agacttgacg caggctacaa tgatccatgg tttgcagcag
    13681 ggaacgaacc gagttttcat attccatgga tttataattg ggtaggttgc ccttataaaa
    13741 cccaattggt tgtaaataga attattaatg aacaatattc aagtgctaat gatggattac
    13801 ctggcaatga tgatttagga accatgggag catggtatgt gttttcatgt attggtttat
    13861 atcctgaaat tcctggtatt ggaggatttg caataaatac tcctattttt gaatcaatta
    13921 aaataaaatt gaaaaatggt gatctcatca ttaatggtgg atcggaaaaa aatatttata
    13981 tcaaatcact gaaaatcaac gataaagttt atgataagac ttggattgac tggaatgatt
    14041 tatctaatgg tgcaatttta aattttaaaa caacttctaa accgaattta acttggggta
    14101 caaaaatgct accaccttct tttccttaat ttatatattc aaaattaatt attaaatcat
    14161 gaaacaatta aaaattaaat caattgtatt tgggatattt gtttttttat ttacttcatg
    14221 taatatctca agaaatgaat atcaaccttc ctttccttta ggcgaattta tcaggcctac
    14281 agggattaat cctgttttat ctcctgatac gacttctgta tttttttgtc caatgaatca
    14341 aaagttagtt cattgggagg aaagtgatgt ttttaatccg ggtgcagtag tcaaagatgg
    14401 gaaaattttt attctttata gagccgaaga caattcttca gttgggattg cttctcgggt
    14461 ttccagaata ggtctggcaa aaacttctga tggctttaca atgaaaaaaa aaccggctcc
    14521 ggtagtattt cccgacaacg ataacaataa attgtacgaa tggcccggtg gatgtgaaga
    14581 ccctcgcgtt tctgtaaccg aagacggaac ttttgtgttg ttctacacat cgtgggatag
    14641 aaaagttgcc agattgtgtg tagccacatc aaaagatttg attcattggg aaaagcatgg
    14701 acctgttttt gcaaacgctt acaatggacg tttcctcaat cattggtcaa aagcatcgag
    14761 tatattgaca actttaaaaa atggcaaatt ggtcattaaa aaaatcgacg gaaaatattt
    14821 tatgtactgg ggtgaatatg taatctatgg agcgacctca gatgatttga aaaattggac
    14881 accagttttg gatgagaaaa atgaattgaa agttttaatg tctccgcgaa aaggatactt
    14941 tgacagtgat ttaaccgaat gtggtccccc ctgtatttgg accaaaaatg gaataattct
    15001 cttgtataat gggaaaaata aacagggaga gggtgatgac aattatgtaa ccaatgccta
    15061 ttgtgccgga caggcattgt ttgatgcaaa tgacccaacc aaacttatta aaagattgga
    15121 taaacctttt ttggttcctc aagaagattt tgaaaaaagt ggtcaatatc ctttcggcac
    15181 tgtttttatt gaaggtttgg tttatcacaa tcaaaaatgg tatttgtatt atggttgtgc
    15241 agattcacgg gtatcggtta caatttatga tcctttttca aaaaataaaa aataaaattt
    15301 aatggttttt ttgggataat tcgtctttat tatacaagtg aaaaaaataa ttcaaatcca
    15361 ttataaataa ttaaatttaa ttcaattcaa ttcttaattt ttatgatatg cgaaataagg
    15421 tattaatttt ttttattcca ttgtgtcttt tgttaggagt atcttgctct gaaaaaggat
    15481 ttttggatga aacagtaaca acagatttaa atcgagatgt agtattttct gatagtactt
    15541 atacagcagg atttttaaat gaaatttata gtcagattgg ttttgatacc gatcctgatc
    15601 gtttttcaac aattgctttt ggatggaaaa ctccttttgg tggtttacaa acagcttgtg
    15661 atgaagcgag attcaaacga tcatcaacta ttacaacaga tgtaatgttt gccacgggta
    15721 ctgttaatcc tatcttagta tcaaaagacg catggcaaaa ttgttacgca aatattcgga
    15781 gagtaaatgt atttttgaaa tatgtggaag gatctccaat agtagacaga attaaaaatc
    15841 aatataaagc agaagctcgt tttttacgtg cctggtatta ttcccttctt ttaagacatt
    15901 atggtggtgt tccattgatt ggtgatacta tatataatat taatgatgat ataaaaatga
    15961 cgagggattc atatgaagat tgtgtaaatt atatcgttag tgaatgtgat gatataatta
    16021 attcaaatgt tttaccaaga cgtagaacag gccgagacta tggacgtatt accgaagcag
    16081 cttgtagagc attaaaatca agagttcttt tatatgcggc aagtcccctt tataatggca
    16141 tagaagataa atatgctcca actgaggaat acaaaaaact cttaggatat cctatttacg
    16201 ataaagaacg ctggaggatt gcttatgagg cagcgcgaga tgttattgcc atgaatgaat
    16261 ataagttatt tgtatggcat caaacagcaa gtgcaaatga tgatatttca ataccagaaa
    16321 ttggatgggg cttttatgct gttacttcga tggcttcaga ttttgccaat ttaacggtga
    16381 ataaagacgg agaggtattt aaagatggtg ctttttgtga aattatttta gataaaactg
    16441 cggcaaaagg taatgcacgt gaaaggttat ttggaccacc tacaagtgga ggaggaggcg
    16501 aaggtggtta tatatataaa gaattagcag atgctttccc aatgatcgat ggtaaaccga
    16561 ttggacaaag taaatatacc tatgatgtat tcaatcccaa tgtaaataga gatcctcgat
    16621 ttgcaaattc tgttctttat gatggtggtt atttttgtac aggggtatcc gcttatactc
    16681 caatcagaat ttcgcaaggc gttggcgcta ccttagatgc tgttcacgtt ggaactccta
    16741 ctggttttta cgtaaaaaaa actgtgagtc gtgctacctg tggtaattcc tttatcggtg
    16801 taccgcaaag tcggccatta attcgttatg ctgaaatttt attaaattac gctgaagcag
    16861 ctaatgaata ttatggacca gattataatg atcaatatac tagtgtttat gatgcgttga
    16921 aagctattcg cgcaagaggt ggaatagaac caggggatga cggtatgtat ggtttaaaac
    16981 caaatatgac acaagatgaa atgagagaag ctattagatt agaaagaaga atcgaattgg
    17041 ctttcgaagg tcatcgtttt tttgacgtac gtcgttggaa aattgccgaa gaaactgata
    17101 acaaagtaat gcatggctta gaaattaccg ttaataatga tggttcaaaa tcatggcagg
    17161 aatttgaagt agcaaaacac acattccgcc ctgctatgta tttttggcct ataccatacg
    17221 atgaagttac aaaatcaaat gaccttttac agaatccttt ttattcagct gaatagtgtt
    17281 aattaataat ataaataacc atatgaaatc taaaatatta aaaactgtta ccttactttc
    17341 tttttttact tttttaatct catgcgacgc atttactgat agtgaattta atgattacaa
    17401 agaaagtgtt aaagatctaa gtggggtatg gcaattaaaa actgtgtcac gaaatggtat
    17461 cgatatttct agcacaatga attttagcca atttcacttg aatttaaatc cgaatggaag
    17521 ctatacttta gagaattatt taccttttgc tgttaaagat aatggggaat ggagggttga
    17581 tgatcctcaa tatccttaca atctaatttt acgcgaaaac aattcaagtg aggatataac
    17641 tattggatta aaatatccaa tagtaaatgg gaaacgcatt atctccataa ctttaagtcc
    17701 tggatgtcaa agcaatactt acgtttatgt gtttgaaaaa attgataata attaaatata
    17761 aggattaaat gaagaatata tttaaaaata agaaaaatac tcgattgata ggattatttc
    17821 taacagtagg atttgtactg tttttaattg gatgtgtttt cattgatagt gtttcaattt
    17881 tgcaaattca agaagatggt acacaagcac ctagaacaaa agcaggttct gtggctacat
    17941 ttattctaac aggtcatatt aactgtcaag aagatcatgg ggacgtacaa tttatttttt
    18001 ctttcttagc acctaaatca tggaatgtta ggcaaaatgc tacagttacc tatactacta
    18061 ctttggctac aaatcctgaa gaagaactta aaatgtcagt tgttcctgaa tcatcattac
    18121 ccaaaaatgg aggaggacgt acctggggag aagctttaat gcaggaatat ggagtaggtc
    18181 ctaacgtttt atctgatatg gaatgggtaa cttttgcaac aaatgataaa tgggcaatat
    18241 ataatggaga taaaccaaat tataccattt atttacggac taatgtagga tatcaaaatt
    18301 tgaaagctta tttaggtttt tttgttaatc atactgatga tgggatgagt actagtgccg
    18361 atcataaaaa agtgaaattt agtgatactc cttttgaggt ttatggaggc aaaggcttga
    18421 tgattgatta tagtactgat cactttaata aagttcaacc acttgcttct ttacaagatg
    18481 attatgtgac ttttagtttc aatggaggtg tttatcaaaa tgacttaatt tcatgtagtg
    18541 aaatttattt tgagggaata gcttatgatg aaaatggaga gataatatct gaagtgaatg
    18601 aaaaaactga caaaacctta cttcacagag agtctatgtt cagtcaggtt tatagtttga
    18661 ctatgtggcc tgctagtttt ttcaatgttc ctcaagggaa agagattaaa aaaattgatt
    18721 acgttttcac caataaagat agatctatta ttataactca atctgatgat gattttgcaa
    18781 taaatcatat accaataaat agtgaaaaag aaccttttgt gtttgaacta ttatgtgatt
    18841 aatattaact gaataaataa atagataaaa attaaaatta atttctatga gaaataataa
    18901 atgccaatat ttaatattat tcatgctaat ttggttggta atgggaattc aatcagtatg
    18961 cgtgaaagca caagataata gctcaaaatc ttccctcaca aatatagtgc tcgatcctta
    19021 tgggaatcct gtctcaaacg ttgagattgg aattaaagga tctgatatca aaacatttac
    19081 taaaatcaat ggaactttcg atttggatta taaagtaggt gatatattga catatagtca
    19141 taaaaatttt atgtattcag aatccaaagt ttctaaaaaa gatgtgaagg aaggtataca
    19201 gatacatttg caagaaaagt caataaaaaa tccttcaaat gttccagaac cttatggaga
    19261 aattattgat aaaaatagtt atttagggtc tgcatcaact atttatacgg atcaactaac
    19321 aaaaacaatg ggtagcactg ttatacctgc tatggttggc cgtatttctg gcctgaacat
    19381 tattcagacc agaggtgccc gtctccatta tacttcggca aactataatg ccgatcttgc
    19441 aggattaata cctgtatttg gtagaagagt ttacagtgac aattctgaat tttctttaaa
    19501 cgcgagagga caagctccca ttgtaattgt tgatggaatt caaagagaat tttattcatt
    19561 agatcctgag gcgattgagt caatttcctt acaaaaagat gccctgtcct caatgtttct
    19621 tggtatgcaa agttcacgtg gagctctaat aattacaacg aaaaaaccaa cacaaggtgc
    19681 tatgaatata tcttttacag gtagatacgg tattactact cctttgcaac ttcctaagcc
    19741 attaaattca tatcaataca cttatttgtt aaatgaagca ctaactaatg atggtaaatt
    19801 accgatttat agttatgaag attttgaaaa atatagaagt ggtaatagcc cttatactca
    19861 tccagatgta aattggtttg atgaattatt aaataataac gcatcaacac aatcctataa
    19921 tttgaatgtt tcggggggaa ataatgtggc tcaatattat gttagcttag gttatatgaa
    19981 tgaagatggg ttgtttataa cttcttctaa aaatccatat aacacgaatt tgaattatca
    20041 gcgatattta attacttcaa agataaatgt aaaagtaacg cgtgatttta ctacaaatat
    20101 aatgttggtt ggaagaattg aagatggcaa tcaaccgggt ggcaatggga gtggctacgc
    20161 tgatttactt aataccatat ttactactcc aaattctgct tatcctatta ccaatcctga
    20221 tggttcgtgg ggtggaaata tttcatttac taacaactta atgtcacaaa caataaattc
    20281 aggttatatt aaagataatg ctcgcgatat tttaggaacg gtaaacttaa attatgattt
    20341 tgacaaattg ataaaaggat tatccattca agccataggg agtattacta cacaatcaag
    20401 atcagcaacc tttagaacaa aaagatcaca ggtatttgct tataaattag atagtgaagg
    20461 gaatcctgtt tatacaatgt atagtacacc ttctcctcag ataaattcat ttagttcagt
    20521 cggaaattat caagatatgt atggtcaact tgccattaat tacgaccgta aatttggttt
    20581 gcacggaata aaagccagtg tgaaaggtga tactcgtact atattgaata attacgattt
    20641 acctgaaatt ccttcaaatg ttatggcaaa tttgtcatat gattttaata acaaatattt
    20701 tgttcagtct actctcaccg aaagttatta taatagatac gcgactggca aaagatgggg
    20761 aacattttat gcttttggat taggctggga cttaagtaaa gaggctttta tgaaatcagt
    20821 aacctggatt gatcaattta aattgcgtgg catatatggt ttaacaggta atgggattac
    20881 caattcaggg tattacatct ggagccaaac ttattcatac catggagcag catggtatcc
    20941 tttagggaca agccaatcaa gtggtttttt tacgactgaa aatcaaccta tagcgaatcc
    21001 taatattacc tgggaaaaag ctcacaaaac aaatgttggt ttagatcttt ccttgttcaa
    21061 ttctaaaata caatttacag cagattatta taatgataac tattttgacc tattgcagtc
    21121 gcgtgggaaa agcattgaac ttataggagc ttcctatcct gatgaaaata taggcaaagc
    21181 aagatggtat ggaggggaat tatcgttaac ttatcaagat aacatcagta aatttaatta
    21241 ttatattaca ggcaattgga atattgtaca gagtaaaata ttatttatgg atgagcaaga
    21301 acaaccttat aattatttat atcaaactgg aaaaccagta ggagcaattt ttggattggt
    21361 taccaatgga tttctttctg ctgatgatat agcaaatggc tatccggtta tacaaggatt
    21421 taaaaatatt caacctggag atgtaaaata tgaagataaa aacggtgatg atgtaattga
    21481 tgaatttgac aggacggtaa ttggtggtaa caaaccttta tccttctttg gaattgattt
    21541 aggagttcaa tataatggtt tcgaattttc aatgatgtgg caaggtgttt ataatagaga
    21601 tatttattta agcgaccgaa actttactga aggtttcttg caaataaatc aacattatgg
    21661 acaagcttat gagcatatgc ttaatagatg gatccctgag aatgcagaaa atgctacttt
    21721 acctcgttta tcggctggta gtaataatta taattacggc aatggttggg gaacgtcatt
    21781 atggctaaaa tcaggcaatt ttattcgttt gaagaatatc aacattgctt acaatcttcc
    21841 cgaatcattt acaagaagtt atttaggtaa tctaagagtt aaaatttttg caacaggtca
    21901 aaatttattt actatgtctg ctacgaattt agttgatcct gaagtgagtt ttactaatta
    21961 tccgattcag cgaaatatta attttggtgt taatgttaaa ttctaatata attatgaaat
    22021 cgaaacaaaa tatatggatg attcctatag tgggaatagt tctgctattt tttgtagctt
    22081 gtactgatag ttatgaaagc tttcccgttg agcagttcac aaaagattac gttttttcta
    22141 aaacagattc aatgggagtt caagcaagaa attttctaaa ttctttgtat ggaatgcttg
    22201 ataatggtca taatggggtt ggaggggatt atttagatgc agcttctgat gatgcaattt
    22261 ccatcaaaca aaacgaacct gatgtttata aactagctgt gggtcaatat agtgctaata
    22321 accgcattag ttcaactatg ggttggggaa aatattatga agccattcgt aaagctaaca
    22381 ttatcatcaa gaatattgat gcagtccctt ttaaactgac ttttgtaaat attaatggag
    22441 aagttaaacc tttgaattat acaataaaag cagaagcacg tttcttaaga gcctattttt
    22501 attttgaatt agtgaaacgt tatggaggtg ttccattgtt aggagattct atctataatt
    22561 tagaggataa tatggaaatc ccgagaaact catttgcaga ttgtattgat tatatggtaa
    22621 gggaactgga tacaataaaa tatgacttga ggagtgtaac aatgcctgat ccatctgtct
    22681 acgctcatgc ggctacaatt gaggctgcaa tggcattgaa atctcgaatt ttactttatg
    22741 ctgcaagtcc attgtttaat gaaaaaccaa taactacaga taataatgct caaaagctat
    22801 tgattggtta tgaaagctat gacattgaac gatggaaaaa agctgctgat gcagccaaat
    22861 attttattga tacttttggt ccaaccggta aaaaaacagt tgatttagca aaaaatcaaa
    22921 tgggtgtttt cctaagtttt tacagtacta ctgcaaatcc agaacttatt ttttttcgtc
    22981 agggaggaat ggataaaagt gtagaaacta ataatgggcc acttggtttt agtggaaatt
    23041 cccttgggaa tgggcgtacc aatcctactc aaaaccttgt ggatgcattt ttaatgaaag
    23101 atggcaagcc gagaggggaa agtactttat atccttatga tcctcaaaaa ccttatgata
    23161 acagagatcc tcgtttagga ttaaccatac tatataatgg agcaaattgg ttaggcagag
    23221 ttttagaaac ttatcgagga ggagcaaata atcctacggg aacgggtgaa tatacaaaaa
    23281 cgagttacta tatgcgtaaa tttatgggaa attttgcgaa tgcaactgaa tatagtaata
    23341 attatcatca ttgggttatt cttcgatata cagaaattct tttaaattac gctgaggcgt
    23401 taaatgaata tttacctgct ccatctcaag atgtgtataa tgctattata tcgttacgga
    23461 aaagggctgg gattccagca ggcagcgata aattatatgg attaaaagcg aatatgacaa
    23521 aagaagaaat gcgtaaagta attcaaaacg aacgtcggat tgaaatggct ttcgaagaac
    23581 aacgttattg ggatataaga agatggcgaa ttgcagaaga tattttcaaa acgcctttaa
    23641 aagggatgaa tattataaaa actctttcgg gcttaaatta ttcagaggtt gatgtgctga
    23701 ctgcaaattt tgaagaaaaa caatatttat acccaatccc ttattcagaa gttattaaaa
    23761 atagaaacat ggttcaaaat cctaaatggt aatttcttat gaaaaaatta ataataacag
    23821 tgatatttct gtatgcctgt ataatagctt ttagtcaaac ttttttacta aaaggcgttg
    23881 taacttcaac tgatggtgaa acacttattg gggttaatgt tataaaaagt ggtacaacta
    23941 ttggtaccat tacagatgtt gatggaaaat ttgaaatcca agttaaggca ggtgataagt
    24001 taaaattttc ttatgttggt tacaaagctc aagaaataat tgtaaagcct aatcaacagt
    24061 atataaatat tcaattagct cccgatcaat ttacaaactt agatgaaatt gttgtaatag
    24121 gttatggaaa ggctaagaag ttaacgatga caggtgcagt tagtgcaatt ccaatgcgtg
    24181 aaatacgttt agtcccaaca agcagtgtgc aaaatgctct atcaggcaaa ttgccaggat
    24241 ttttttcagt acaacgctct ggtcaacctg gtaaagatgc ttcagacttc tttattcggg
    24301 gagttagctc tttgaatgat gctggtaatc aacctttgat tatcgtagat gatgtacaat
    24361 actcatacga tcagttacaa cagatcaata taaatgaaat agaaagtatt tccatattga
    24421 aagacgcttc tactactgct atctatggca tcaagggtgc aaatggggta ttagtggtaa
    24481 aaacacgaag gggagaacaa ggggcacctc aaattaacgt aagaatggaa agtgggattc
    24541 aaacaccagt tagaaaacca aaatttctta attcatacga aactgctaaa ttggtaaatg
    24601 aagcgtacca aaatgatggc ttaatttctc aaattccatt ttcagaagat gatttggaag
    24661 cgtttagaac tggttctgat ccttacggtc atcctgacgt taattggtat aatgaaattt
    24721 tcaaatcaat cgcttcacaa aataatgtta atgttgatat ttccggaggt tctaaaaatt
    24781 tgaaatattt cctttctaca ggttattttt tacaagatgg tttggttaaa aactttaata
    24841 gtggacaaaa tgatgtaaat acaaattatt tttatcgtcg ttttaattat cgaaccaatt
    24901 tagactttga tgttagcaat aatttaacca tgcgtttaga cttctctact cgtttcatga
    24961 atatcaatga accaagtcgc ttaaatgcta ctggagaaat ttacaatttt gagaaaatga
    25021 ctccttatgc tgcaccggtt ctcaatccca atggctcatt tacatatttg gatacaaaag
    25081 gttttttacc gactttaaat gctcggttgg cgaatgaagg atataaaaga acaaaaagaa
    25141 acgatattaa cgtattgtat ggtattattt ataaaatgga ttggttaaca aaaggacttt
    25201 ctggaaattt tagattagcc tactcaagta tagatgaaaa ctatagaagt gtttccaggg
    25261 caagtgatgg ttatcctacc tatcgttatc atccagaaac agatacttat accattaacc
    25321 ccaaccaagt gtatgcttat tctccttatg gagttacttc aggtgttggt caagcacaaa
    25381 atgacctcaa tattcaagca tcaatcaatt atgctcgggt atttaacaag gatcacgatg
    25441 taagtgcaat gttgctttat aatagagaaa gtcaaacaaa tgaagctgct gcttctgtac
    25501 cgagtaattt tgaaggtttt acatcaacac tgggttataa atataaaaat aaatatttaa
    25561 tagattttaa tttagcttat aatggttcag atcgttttgg aaaaaataat cgtttcggat
    25621 ttttccctgc attaggagtg ggatatacaa tttcagaaga agatttcttt aaaaataaat
    25681 tcaataatat acaaatgttg aaaattagaa cttcttatgg attggttgga tctgatattg
    25741 ctcctggtgg acgttattta tatcgtcagg tatatcaaag tggtaatggt tattactttg
    25801 gtgaaaaccc tcaatatcaa gcctcctata aagaaggaga tttaggaaac gaaaatgtta
    25861 cttgggaaaa agcaaaaaaa tttgacattg gtttggattt agttgcattc aaaaatttat
    25921 catttacaat tgattatttc catgactatc gatttgacca gttagttact agatatgata
    25981 tacctcttat ccttggtata ggagtatccc cattaaatgt agctaaaaca gtaaatcaag
    26041 gttttgatgg ttctcttgga tatcaaacaa aaattaatca agttaatttc cttacaaatt
    26101 ttgtattttc ttatgcaaag aataaagtaa tatacaaagc ggaagctcaa caaagatatc
    26161 cgtggcttgc acaaacagga aaaccgatta atcaaccttt tggttataaa tttataggtt
    26221 tttatacacc ggaagatatt gagttgataa ataacaatga tcctgcagct cctgcggtac
    26281 caaatactga tacgcctgtg caagctggtg atttaaaata taaagatttg aataatgata
    26341 gagtaattga cgattatgat aaatgtgcaa ttggcaaacc aaatttaccc actacctcta
    26401 taggctggac tattggtggg aattggaaag gattctcgct tagcatatta tttcaaggat
    26461 cttttgatta tagttttagt gtagttggaa caggaataga atcattcaag agtcagtttc
    26521 aaccagtaca tttaaaaaga tggactttag aaaggtatca gaacggagaa gacattcaat
    26581 ttccacgctt aactaccatt ccatctacag taaatagtgc tgaaggttac atgtctgatt
    26641 tctggttaat tgacgcttgg tacattagaa tgaaaacagt tgattttgct tatcaacttc
    26701 ctaaaaaaac actgccaaaa ttcattaata cagctagatt ttatttaact gcttataact
    26761 tatttacatt tacaaactat aaaaaatatc aacaagatcc agaaattcaa acaaatacag
    26821 caggagatgc ttatatgaat caacgggtag taaatttggg cgttcaagta acattctaag
    26881 agtttaaaat aaaaaaggtt ttgaaataat aatcaaccgt caccatttat attgtttagt
    26941 tacgcttttt taaatggcga cggaaattta cgcaaagttt aatgttaatc tatatactaa
    27001 aatgaaaaaa ttagttattt tattttttat tattgtatgt tctggagtga atttgttttc
    27061 acaaccagtt agcaaattta aagatggtga tagagtggtt tttttaggaa acagtataac
    27121 tgatggaggc cattatcatt ctttcatatg gttgttttac atgacaagat ttcccaatat
    27181 ggatttgctt attatgaatg caggtattgg gggagataca gcctatgata tgtataaacg
    27241 tttggatgat gatgtttttt gcaagagacc aacagtactt gtggtgactt ttggtatgaa
    27301 tgattctggt tattttgaat ataatagtga taaggcaaaa gaatttggag aagaaaaata
    27361 tcaaacttgc tataagaatt ttaaaatgtt agaaaagcgt ttgttagagc ttcctaacac
    27421 tagaatagtt atgatgggaa gctctccata tgatgaaaat gtaaaaatta atggtaatat
    27481 accttttaaa ggtaaaaatg ctgtaatgca gcgaatagtt gaatttcaaa aacaagccgc
    27541 tcaagaacat aattgggaat ttcttgattt aaatcaaccc atgactgcta ttaatcagaa
    27601 atttcaacag accgattcta cttttacttt atgtggttta gatagaattc atcctgacaa
    27661 tgatggtcat atggttatgg cttatctttt cttaaaagaa caaggattcg caggtaataa
    27721 agtggcagat attgaaatca atgcttcaaa aggttctata attaaatcaa caaactgtgg
    27781 aataagcaac ttaaaaaaag atggtaataa aatatctttt gattatttag catattcttt
    27841 accttatcca ttagatacaa tgcctcgtgg ttggaattca aaaaaatcgc aagcacaagc
    27901 gataaaatat gttcctttta tagatgaaat gaatcaagaa attttaaaaa ttactggctt
    27961 aaaaggtaat tataaattaa tgatcgatga caatgaaata ggaacgtggt cttctgatga
    28021 ttttgctaag ggaataaatt tagcaagtga gactaaaact cctcagtatc agcaggcatt
    28081 agcagttatg tatctaaatg agtaccgttg ggaaattgaa cgaaattttc gggagtatgc
    28141 gtggatacaa tttgatttct ttcaacaaaa aggattactt tttgctgata atcaagaggc
    28201 agtaaatgta cttgataaag aaataaagaa aactaatgat atttggttga aaatgcatcg
    28261 tgataattat gctcaaatga tgcacaaatc tgtgcgagaa gcacgagaag aagagatgaa
    28321 gctacttatt tcaaagattt atgaaattaa taagcctgtc acaagaaaaa ttactttgaa
    28381 aaaaatttaa tttttaactt tttaattcat tcataaaatc tactttgaat tttattgatg
    28441 aaatttttgt gattattaaa agattaattc cattttttta acggttttaa gtagtgaaat
    28501 cgtctatatt aattaaatat aaaattatat atctttttat ttagttaaac atttatttat
    28561 taacaattta aaattaatca ttatgaaaaa aatttttatc tttttattgg caatttttgc
    28621 agcttcagtg acttatgctg gcacatgggt aattggcgta aatcaagtgg atcgcttttt
    28681 aaaagcaatt gaaaaaggaa caggagatga acctcctact gtcccaatga aagttgaccc
    28741 agtaacgggc gagaatacaa ttaatacaga ttatgatgtt tggaaagatt ttccaggaat
    28801 aacttttact tctgaaacga ttgtgccaac ctatcttgca gatcaggcag atgcagatca
    28861 ccattggtat ggtattaagg aaattaatta tgctaacaaa ggttacaaac agaaagaaga
    28921 cgttgctatt gaatttgttc aaccaagcac tgtagaatgg gattattctg gtgttccgga
    28981 aggttattct agcctttcta attattttga tagaaatgat ctttcatggt tagaaaacat
    29041 tgatttatca ggaaatgatt tgaccaatat tttgattgat ggaggtccat ataatgctat
    29101 gccattaaag actgttaatt tatctaataa cccaaatctt acaacgcttt caatcgtaaa
    29161 ttgtacgaat ctggaagagg taaatttatt gggaaccggt ctaacacctg aagcatttga
    29221 aattgtgaaa gagggtattt tagaatcttc accttcagct aatattttat atgcttccaa
    29281 tgaagtgaaa tcaatcaaaa ttgacgatcc tgtaatagtc gttcaagggg gtaatattgt
    29341 tattaaaaat aaaaaggtga atgatcaggt aattattttt gatttatcag gaagaaaaat
    29401 aatagaaaca tccaatgatg tgattaatgc caattcaata ggcaaaggag tgtatatggt
    29461 gaagataaat aattttgtaa aaaagatttg tctgtgattt atttgcaatt gtaaactaaa
    29521 gggttaaaca attaattttg gcaaattttt taattttttg caacactcaa ttttgtatag
    29581 gtagagcatt tttgggtgtt gcaaattttt ttgacattat ttttgataat aatttaaatt
    29641 gataaattgg cattgaaagg cgaatccttc ctgtgttact aaaaatcact ttattcattg
    29701 ttctcaacga ttattcattt ttattgtgat catttattca atcatgaaaa aacttaatct
    29761 tctttttata tctctttttg tttttttgtt caatgatatt caagcacaaa cttcaattgt
    29821 ctcattgaat agttctaatc ccaatattat ttgggaaata aaacctcaag cagatgttaa
    29881 cttttccggt aatcagattt ccattcccgg ttttcagttt accgattatg ttaaaggaat
    29941 tgttcccggt gttgttttta cttcttatgt ggcagcaggg aaagaaaaag acccaaattt
    30001 ttcagataat atttataaag ttgacgaaat attttacaat cgtccttttt ggtatcgaac
    30061 agaatttgaa attcctactt cttataaaaa aggacaaaga gtttggttac attttaacgg
    30121 aacaaatcgc tttgcagact tttattttaa tgggaccaaa atttcaggga caccaacttc
    30181 cattaaagat gtgagtggtc atatgttgcg ttccaaattt gatgttacgg atttaataaa
    30241 tgtaactgga aaaaatgctg ttgctgtact aatctgggat gcggatcaaa aaaaacgaag
    30301 agactcgaaa gaagcttttg gtgtagcaag cagcccaact tatctggcag cagcaggatg
    30361 ggactggatg ccttatgttc cgggtcgttt agccggaata acaggcaatg tttatctgaa
    30421 aattacagga gatgtaataa tggaagatcc atgggtaagg tctgatcttc ctgccctcga
    30481 tgtggcagaa atctcgatta tgcccaatat taaaaatgtg tcttcaacaa atcaggatgt
    30541 taccttgaat ggcttaatcc aaccgggaaa tatttcattt acaaaaaata tcaccctacc
    30601 caaaggaaaa acgacaaata taacaatcaa taaaaatgat ttttcacaat tggtgataaa
    30661 caatcctaaa ctatggtggc ccaacggata tggtgaacca aatttataca cttgcaacct
    30721 gaaatgcata gtggatggta aaatttctga tgaaaaagaa attacttttg gcatcagaaa
    30781 gtacgaatat aaaatgatca acaataaagt aggatggcct gtgttaaact tatttattaa
    30841 cgggcaaaga ttatttgtaa agggaggaaa ttgggggatg agtgaatatt tattgcgttg
    30901 ccacggtgat gaatatgaaa ctaaaatttc tcttcacaaa gatttgaatt ataatatgat
    30961 tcgttgctgg acaggatgcg taacagatga cgagttttac gattattgcg acaagtacgg
    31021 cataatggtt tgggatgact tttggttgta tgtggctttt aatgatgtgg ctcaaccgga
    31081 agctttcaaa gccaacgccc tcgataaagt tcgtaggttg agaaatcatc cgtgtattgc
    31141 agtgtggtgc ggagcaaatg aaacgcatcc gaaacccgag ttggataatt atctcaggga
    31201 aatgatagct aaggaagata ataacgatcg tctatataaa tcgtgttcca atcaggatgg
    31261 attatcgggt agtggctggt ggggaaatca accgccaaaa catcattttg aaacttccgg
    31321 cagcaatctg gcatggaatg atcctccgta tccgtatggt tcggattatg gttacggttt
    31381 tcgtagcgaa ataggaacgg caacttttcc tgtttatgaa agtatcatca aatttatgcc
    31441 tcaagatcag ttgtggcctt tgccaacgga tgaacagcta agaaatgacg acaatacgat
    31501 ttataacaag catttttttg gaaaggaagc ttcaaatgcc aatccggtta attacaaaag
    31561 atcagtcaat actgaatttg gagaatcagc aaatttgcag gaattttgtg aaaaggccca
    31621 actcctcaac attgaggtaa tgaaaggaat gtatgaagct tggaacgaca aaatgtggaa
    31681 tgatgcttcc ggattgttaa tttggatgag tcagtctgct tatccttcct ttgtatggca
    31741 aacctacgat tactattatg atgctacagg cgcttactgg ggagccaaga aagcttgcga
    31801 accgcaacac attcaatgga attgctcgag caatagcgta aaagttataa atactacttc
    31861 caaagccctg acaaacgtaa aagcaatggt aaatgtttac gatataaatg gaaaagaaat
    31921 cggaaaatac aggaaatctg cagttttaaa tgtaaatccc agcaatatta acgaagcttt
    31981 tgttataaat ttcaatactt ccaatcttgc actgggtaaa aaagcatttg cttcctcgtc
    32041 aacaaaaaat atggatgctg ctttaatcac agatggtgca agtggcagtc gttgggaaag
    32101 cgaatataac gatccgcaat ggatttatgt ggatttaggg aaaaaacaaa agattgaatc
    32161 tgtggtatta aaatgggaag cagctcatgc caaagaatat gaattgcaag tatcaaacga
    32221 tgcaaataat tggacaacta tttataagca acaagattcc aaaggtggaa ctgaaacaat
    32281 aaaaattaaa cctgtatcgg cacaatttgt caaactaaac tgcatttcga gagcaaccat
    32341 tttcggatat tcactattcg agttggaagt ttacggaaaa atgcaaaagc agcaagaatt
    32401 atcaccctta cagtttgtta aattaaaatt aaatgataat caaggaaatt taatctcaga
    32461 aaatttttat tggagaaacg gtatccatga cgaagatttt acagctttga acaatatgaa
    32521 agaagctact atttcgtgta aattgataga taaaaggcag gaaaataata aagaaaaaat
    32581 aaagctactg ataaagaacg attcaaaaat tatagccttt gccaaccgaa taagattagt
    32641 gaatagtgtt acaaaagaac gcgtgttgcc ggtgatgatg aacgaaaatt atttctcact
    32701 gcttccgggt gaagaaaaaa taatcaccat cgaagcagac agtcaacttt tgaaaggtgg
    32761 tgctgatata ttgttaaaac aatttagaaa agaagaaaga aatattttga aaataattga
    32821 ttgaagaaga gaagtatatt ttctcctttg taaaaacatt attctttgaa agcgaaaagc
    32881 ttaatttttt aactttagaa aaaaataaaa taacatgaaa aaaaatattc tttttttatt
    32941 ttgtttgtta gcatttaata ttttgaaatt atctgccggc acatgggtta taggtgtaaa
    33001 tcaagtagat cgttttttaa aagccattga aaaaggaggt ggagaagaac caccggctat
    33061 acccttaaaa gatgatggaa caattaatac aaattattac ggagtgtggg aaatttttcc
    33121 aggtatcact tgtacttctg aggacattgt accattttat atggaaggtc aaggttccga
    33181 tcatcattgg agagggatta aaaaaatcaa ctatgctaat aaaggttata aacaaaaaag
    33241 tggtaccgtt attagctttg tacaaccaag cttgaacgca tcggattatc ctgaaattcc
    33301 taccggttat accagccttt ctaattattg ggatcgaaat gatctttctt ggttagaaat
    33361 cattgatttt tctgggaatg actttgagaa cattgaagtt gatgggcata atattatgcc
    33421 attaacggaa atatcattag caaataatcc tcatttgaaa agtttaataa taaaagattg
    33481 cccaagttta acaaatgtta atatcaccgg atgcaattta actttttcgt ttatttccaa
    33541 cttacgaaat ataataaatt tatcagctaa tgcagaattc ttatatgaat atcaaggaga
    33601 aattacttct tattatgccg ctgttgattt aactgaatta cttgaaaaaa atggagaagg
    33661 aagtcgaata aaatcatggt caatccagcc aataaaaaat gaaggaaata tttatacctt
    33721 tgactcaagt cacgtgggag aggtcgtacg tgtttctctg gaaaattcag attatcccgg
    33781 tataattata tcctacgata taaacctgac aagtgctatt ccatccacta tctcaataaa
    33841 tgctacttat tgtaaagtaa aaatacaaaa tctgacaaca gggaataata atttttctat
    33901 tggagatgaa gcattaatta cagtataccc ctattcttgc tctgttctcg aatcatttac
    33961 aattgatgga gaggaagtta cattggattc taaatatcaa tattatttta ttgtaaataa
    34021 aacttttcac cagattactt caatctttaa aaaagatgat atgccaactg gacaaacgtc
    34081 aaattatttg cgaaatggga gttttgagta tggtctaaat ttagattggg aatataatat
    34141 tggagaggga acttcggcag agtttacgtt ggagcgaggt agtaatgtaa tagatggcaa
    34201 tgttgcttta agggttgatg ttaattcttt aaaaactcct aattcagttt ctgctaaaac
    34261 acatgttgca gttggttgtg attctcttta tctattacat ttttgggcaa aaggtccaga
    34321 agaaagtaaa ttatatgtcc agatagatgg ggcagaacaa aatggaatac aatatgaact
    34381 tcacgaagga gccactgcat atttttatcc tattaaaatt gataaaacca aagccaatga
    34441 aggattgaca atcactttct attttcaaaa tgattttaca aaagtaaagg ttgcggatcc
    34501 atgtagtgtg acaacttatg caggagcaac ttattatctt gatgaccttg agttagttga
    34561 ccaatttaat aatttgactt atgatgttta caatacttat ctctggaatt ataatcatag
    34621 accaaataat gaaggaaaaa tgtgggtagc aggagataat agtgtttcat atgatttacc
    34681 agatggaagg aaaatgtggt ttttcaatga ttccttttat ggggtgctac atcctgaaag
    34741 taatcgactt gttgacgtag gtcggtttgt tcgaaatgcg gtagttgttc aaaacattga
    34801 tggaacatta acaacttatc ctgttactaa tcagggaggt caatggacat attttcgggt
    34861 tcccgatgat gcagtaattt ataatgatga tggaagtgtc aaaaatatat tttgggttgg
    34921 tgatgcttta atggaagata atcaaattaa agtgtattta attgaagttt atggtgaagg
    34981 gcgatcgtat ttaggaaaat tctcttatcc tggccttgaa tttttaggtg tcgaaaaaca
    35041 agaaaatttt tgtagaaaat atgagacttt ttttgtggaa gataacaaaa tttacttata
    35101 caaaactgaa gatgtaggta catggggacg ttatatgcac gttgccagag ctgatttggg
    35161 agacttgagc ggaaaaaaag gaacttggga attttggaat ggaaccgaat ggtcaaaaga
    35221 tagtactcag acagccagaa tttatgatat ggcaagtgat ggcgttattc gtttagaaag
    35281 tggtaactat gctcatgtat ccatgccagt attaagtcca gaagtttatg tttcatttgc
    35341 tccggcccca caaggtcctt ggacaaacaa aaaattaatc gctgttggtg ataaaagtgc
    35401 aaattattgg tattacatgc ctaattttaa cgggaaatta ccaaatggga aatatgccat
    35461 ttcattctca gcaaattata actattgttt gtttttttgt aaagattgtg aaacctcttc
    35521 atttgttgac aaatattggt atcgtccaag atttatgcaa gtagacttac ttgctttatc
    35581 accttacaca aaaaatcaca aggattgcgc aggcgtagaa aatggcaatg cctattacga
    35641 cacatgcggt cattgtgtgg gaggaaccac tagtatcgaa ccttgtttaa tcggagtagc
    35701 aaaactttat gccaatgcaa attattctgg tgttgggatt ggactgaatg ctggagaata
    35761 tacttccaat gatttgtcaa atcttggttt tgaggtggaa gctttatcat catttaagtt
    35821 ggatcaagga tatgttattg aattgtactc tgaagataat tttcaaggag aaatgaaatt
    35881 atttgaaagt agcgtaaagg atttatcaga aaaaaatttt gacaatcaaa caaaatcatt
    35941 aattattcgc cgaaaaggaa ttgaaaactt aagtggtatt tacgctattc agaataaaca
    36001 aagtgagttg tatcttgatg tagaaggtag taatccagca aataatgctt caatcattca
    36061 aagtttatat aaaaatagtg attttcaaaa gtttaaattg aataatctgg gtaaagggtt
    36121 ctatgctctt gaaaatatgg gaagcgagaa agtccttaat atagctaatc aaagcaaaga
    36181 ggaaagagct tatgttgaac aatgggacgg gaaagaaatt gatatcacaa cgcaaggtgg
    36241 tgaaatttca gctcaatatt cagattcacc agcaggagaa gatataagta agatcataga
    36301 caaaaatcct gcgaccaaat atttaacttt ccatagcaaa gcatgggtac aatttaaatg
    36361 ttctcagcct tggatcgtaa ttaggtacac tttaacttct gcaaatgatg cgcctgttcg
    36421 tgatcctcgt tcgtggacat tgtctggatc gaatgatgga atttattgga caatacttga
    36481 tacacaatca ggaataagct ttacaaaacg gtttgaagaa aagtcatttc ctatcaataa
    36541 tgaaataagc tatttatatt ataaaataga tatgacatgc agcagtggtt caatacttca
    36601 ggtagcagaa ttaaagctat ttgccaaaac aaacatttct gatggtgatt attttaatag
    36661 tcaaaaattt gttatacaag atggggggaa tgattttgtt aaatttataa ataagaatag
    36721 tgatttgatg ttggaaatcg ttgatggatt taccaatgaa ggtgaaaaag tttggcaaaa
    36781 ttatgactat ggccaatggg gaggtttttg gaaattagtt gatccgacta gcatttcaaa
    36841 tgaagtggga acgttattgt ccaataaaga tatattgatt tatccaaatc ctgcttctgc
    36901 ctatttaata attgaatcta atcaatcaga aattattaca agaatcattg ttgcggattt
    36961 gagtggtcgt attatgcaag ataaaaccta taataatagt aagattgtat tatctttatc
    37021 tgatttacaa aaagggcttt atttgcttaa aatcaatact gatagaagtt cttatggaca
    37081 tatcataatc aggaactaaa atattttaag cgaaaatctt aaaaaaatta tgtttaaaaa
    37141 aataattgtt tttttgcttg caacaacttt ttccttacaa ctttattctc aatatatcta
    37201 ttcgttagtt tcatcggaca aaatcttatg gaaagttttt cctgttgata tggttactgc
    37261 ttctatttct gatttatcaa ctccagaatt tgatgtttca tcatgggtaa tagctaaagt
    37321 accaggtaca tattttgttg attatgtgaa tgctggtctt gaagccgatc ccaactatgg
    37381 agacaacatt tacctggtgg atgaaaaaaa atacaacaag gaattttggt atcgtactga
    37441 atttgaagtt ccagctgatt ttgtcaaaga taaactttgg ttaaatttca aaggagtgaa
    37501 taaagaagga acttttttcc tcaatggaga attacttggt agcgtaaaag gccacatgtt
    37561 gcgttccgta tatgatatta ccaatcaagt aaaagtgggt aagaatgttt tacttgtgaa
    37621 aaccaatgtc ccggatgtaa gaagagaaag gcagccaacg ggagtatttg acacatgggc
    37681 aaattttgct atgcccactt atatgggaag tgccagctgg gactggatgc catatgttcc
    37741 tggtttaaat tgtggaatta caggcaatgt ttatctttca actactggca tggtgaaatt
    37801 ggatgatgtt tgggtgcgtt cagaccttcc tgatgacagt cacgccaatt taagtattca
    37861 agctaccatt aataatacct gtaatcaatc cgtaactgga atattaaacg gaactatcat
    37921 gccaggtaat attcattttt caaaaacggt ggttattgaa gcaaatcaat ctttattggt
    37981 aaaaataaac caggaagaat atgctcaatt aattataaat aatcctcttc tttggtggcc
    38041 cataggttat ggtaatcaaa atctttatac gttaagttta gaattcaaag ttaacaatga
    38101 ggtttcagat gctcaggctg ttatttttgg catcagaaaa tatgaatata agaatgaaaa
    38161 tggtgccttt accctgtaca ttaataattc caaagtcttt gtcagaggtg ggaactgggg
    38221 aatgtcagaa tatttgttac gttgcaaagg tgaagattat gatactcgct taaaactcca
    38281 tgcagatatg aatctcaaca tgattcgttg ttggacaggt tgtgtgacag atgatgaatt
    38341 ttatgattat tgtgacaaat atggaattat ggtgtgggat gatttttggt ttcacggcag
    38401 tctctttccg cctagcgaag atatttttat gagcaatgct atcgataaaa taaaaagatt
    38461 acgtaatcat ccctgcattg ctgtctggtg tggtgcaaat gaaggcattc ctgctgggaa
    38521 tttagatcaa ttacttaaaa atgcggtagc cgattacgat gggaatgaca gacattatca
    38581 atcttgttct aactcaggag gaggtttatc tggaagtggc tggtggctta atatgcatcc
    38641 tacaaattat tttaccggac caggcagtgg aggaggtgat tggaatggtt ctggaacctg
    38701 ggcttttaga agtgaaatag gtatggcaac ttttactacc ttcgaaagtt ttcaggagtt
    38761 catgcccgtt gatagttggt ggccccctaa aaatgagatg tggaacaaac atttttttgg
    38821 ctctcaagca caaaacgccg gttgtgatac ttatttcaac acgctgagcg acaattatgg
    38881 tacttcaagt gaaattgatg agttttgtga aaaatcacaa tatcttaatc tggaagtgat
    38941 gaaagctatt tatgagggat ggaatgataa tatgtggaac aatgcaacag gggtattaat
    39001 ttggatgagc caatctgctt atccttcatt tgtatggcaa acctatgatt attattatga
    39061 tgctaccggc tcatattggg gagccaaaaa agcttgtgag cctctccata tccaatggaa
    39121 ttgtgctaac aactcagtaa aagtaattaa tacaacatta aacaattatg cccagctcaa
    39181 agccaaagct tcagtttaca acttgaaggg tgaagaatat ttaccccttt gtatggaaaa
    39241 attagtagaa tcaaaatcca atactgctac agaatgtttc gtgttaacag aaaaaaattc
    39301 aatcaattta gcattgttta aaaaagctta tgcctctgtt aatgaaaatg atggtgaagg
    39361 tgttccatcg cgagcagtag atggaaattt atctacccga tggggaagtg gttatcaaaa
    39421 caatgcatgg ttttatgtag acatggaatc tactattctg attaataaag tgaaaatata
    39481 ttgggaatat gcatatggaa aaaaatttca aattcaggtt tctaatgatg ctaataactg
    39541 gattactgta tatacccaaa ataacggaac gggaggcgtt gaaactattt cattcgatcc
    39601 tgttttagcc cgttatgtaa aatttcaagg gatagaaaga ggaaccatgt ggggttattc
    39661 gttttatgaa tttgaagtat atgctccaag ttcgaatgac gaaaataatg aaaattctgt
    39721 agagctaaca aaattgaatt ttatcaaatt acaattgttt gatgaaaaca ataatttgtt
    39781 atctgaaaat ttctattgga gaaacatgga tgaatacaat tatcaggatt taaatacgtt
    39841 acctaaagca gatgttacca tcatcgaaac ccatcgaaag gaaaacggaa aatattttgt
    39901 atcttgcgtt gtgaaaaaca attcatctac catcgctttt gggaatcggt taaaagttgt
    39961 aaataagcaa acagggaaac gaattttacc tattttcttg aatgataatt attttacatt
    40021 aatgccaaat gaaagtaaaa atattgaaat agaattttca gaaacactta ttggcaagga
    40081 taattttgaa gttgttctga aacagtatgg aaactacgat tcaactccag ttcaatcaaa
    40141 tgttgagaag gaaagtatga ttagttcaga tgtgctgcca gtgtatccaa atcctgcctc
    40201 tcaggttata actttgggga acccggcaac aagaatcagt gaagtaaaaa tatacaatat
    40261 ttttggaagt ttagtgcatt ccgccagtta ttgttccata atagatattt caaaactttc
    40321 tccaggtatt taccttgtga gcgctattat tgataataag ttatacacca gccgatttat
    40381 aaaggattaa aacgaaaaag taattatttt gtgaagagac tgatttgtac tttaattggt
    40441 agtgttattt tttgtggatt ttattcatac tcagctgttc aagaattgag caaagaagct
    40501 actatttcgt tgttgatagc ttcaccaaat cctgatcagc tggtttcgtc ttatggacat
    40561 tccttgcttc gagtaaaaga tcctaaacta aacattgatt atgtatttaa ttatggcaca
    40621 tttgatagaa ctttgtctgg tttcgaaaca tttatgggaa ttgctacagg aaaattaaaa
    40681 tatgagattt gggttattcc ttatactgaa tattacgaaa cgactctgaa agaaaacaga
    40741 aagttagttg aatatacgtt caatttttca gccgaagaga aaaatacaat atggcataac
    40801 ttaatttcta tcgttaagaa taaaaaccag tacctatttg atttcttttc ccaaaactgt
    40861 actacttttg taagagactt gattacctat aatttaggca aacaaatttt gttgccgtct
    40921 gatttaggac aaaaaactta tcacgatata aatataaaat ataccaaaaa caaactgtgg
    40981 tatgtatttt tagtagattt gatatccggt atgaatttgg ataaaaaagc accttcttac
    41041 gaaagccttt atgaaccgaa agaattggtg aatgcatggt ctaaatcttt catacttgag
    41101 gataatggtg aaaaaaaacc tatatttgct tcagcttctc ttttaattaa tggtaaaaac
    41161 ctgattaacg aaacaaaaac accttttatt atctctccat tattttttag tcttgttttg
    41221 cttgttttag ttttaagtct ttcgatcatt gaatgggaaa caggcaatta ttatcgatgg
    41281 tttgatattc tcatttttgg gttcactggt tttttggggc ttctttttta tctgtttaaa
    41341 tttgcttgtc caagatggta ttttttaatg gattggaaaa tgttttggct ccatcctttt
    41401 catttattag cagttttctt tttaatcaaa tcacttaata agccattaaa gatttatcat
    41461 tatattaatt cggccatact tgcttttttg attgtgggag tatttttttt acctcagcat
    41521 tttaatccgg caatcatcct cctcataatt tgcttgttca ttcgttcaat attttttgtt
    41581 ttaagagaaa gaatcccaaa taagaaaaaa acaattagaa agttatttat tttgaaaagt
    41641 tcctcacata atcagtcttc tgttaaatta cttaaaagta aggaattttt agaaaacatc
    41701 aaatttcctc tgctatcaaa taagactgca ttttgtttta atatgagctt tgtctcaaat
    41761 tcaactaact ttttgtctta caagagagct aaaattgatt ttttatctgg tgttatttaa
    41821 gaattttttc ttgatccttt cgcttggtaa ctatttgaaa ttgaattatt tattacacag
    41881 aaaaatctac cgagagctac aaaaattttt gggtattcca ctctgttaac ttgccgattt
    41941 gaaaatcgca ccctctacca aaaggtacca gaaattctta ctgcaaatca actttctaca
    42001 agcattatgt cttgaatggg gcattccaag ctaccaacta caaattatta actaaacaaa
    42061 aaaatttatt tcacagattc aacccgaaac caatctacat caacataacc agcatcgttg
    42121 gagaaatgag gccgtttaca aaagattccg actttcgctc ctatccaaag tccttctttc
    42181 gcaatgaacg atttcccaat cgttacatat tttttcccgt cccaactgta actgaactgg
    42241 caattggcaa gtggttgaag aatttcttcc ctgttttttt cttccgtttg tctaatttcc
    42301 actctaaaat ataacggtat attttttttc aataaaacag aatcggtaac cacttcagca
    42361 ttcccctttg aagcttcttt acaaatcgtt tgcttgataa ataacccgtt ttgggtactg
    42421 tcaacggtta ttgcagcgta atcctgcccc atcaccacca aaccggcaga ttctcctatt
    42481 ttggaaggag aaaatacaag ctttgtcgtc gctttgaaat tcggagccgg gaacttttgc
    42541 aataataaat tgggcgcatc ccataaattt tttccgtttt catcaggttg ccatgcaaaa
    42601 agacgcaaat ttccgctttc gctgtcgaga cgataccaca atggattgtt atttgcctgc
    42661 cattgccatt gcaatccaag cattggagta ttgaattcgt cgctttccat cggattttca
    42721 ggcgaataaa ctttgccaat atttggtttt ttccacgtca acacaggatt gccacaaccg
    42781 tcgccatcct tatcttcgcc aatcaccggc caatcattta tccattgaag cggttccaac
    42841 caaacgaccc tgccatacgc atatttatcc tgaaaatgga taaaccaatc ttctttccca
    42901 tcaggtgtat tgatccatgc tccctgatga ggcccgttaa tagctgtttc cccctgcgag
    42961 agaacaattt tagattcata cggaccgtaa atattttttg aacgtaaagc caattgccaa
    43021 ccgggtttta cgcctcctgc cggggcaaaa atgtaataat atccgtttcg tttgtaaaat
    43081 ttaggccctt ccactgttgg atttgccgga tgaccatcga aaatcagtcg actttcagaa
    43141 agagcttgcg ttccatccgg tgtcatttca aaaattccga gaacgctttt cattccggct
    43201 ctgctcccag caaagccatg caccacataa gctcttccat cttcatccca aagcggacaa
    43261 ggatcaatca gcccttttcc tttttttacc aaataaagag acgaccagga accttttatg
    43321 tcttttgctc ttgtcatata gattccttcg tccggatcgc caaaataaat ataaaattga
    43381 ttgtcgtgat agcgaatgct gggagcccaa acaccatttc catgctgcat tttattgtat
    43441 ttttcagcag gagtcagatt ttggatcgca tgtccgacaa ttgtccaatt taccaaatcg
    43501 ttggaatgga gaatgggcaa accgggcgta ttggcaaacg acgaagatac catgtaataa
    43561 tcattgccaa ctctgcacac atccggatcg gaataatcgg cataaataac aggattttta
    43621 tattttccat tccccaaatc gggcgaccaa acttgcgaaa cataaggttg ttggttttca
    43681 cgcaattctt tcatcaatgg cgtaattttc tgttccttca atccttcgac aaaaaaaccg
    43741 gctacaatac gtgctccctt ttcgttaaag tgggtgttat cgtcaagccc tttgggataa
    43801 agtttgctca cacccgcagg tattttgtga aaatactgtt tcgacttttc agggccttgc
    43861 tgttcgagcc attgagttgt ttgcttttgc atatcaatca acgttacatt tttcattctc
    43921 gcaacttcac gacaaatgtc gggataaacg ccgtgtgtat ccaccaatct tccttctgcg
    43981 tcaaattttc ttcgcattac cgacgtacaa atgattggaa ttgccccttt tgcttttact
    44041 tcgtccacaa agtgaatcaa attggttcga taatcttcag gtgaagtata tcgatcgggt
    44101 ttatcctttg ctccatcgtt gtgtccaaac tgaatgacga cataatcgcc cttttgaact
    44161 tcagaaatca gtttattcca ccaaccttcc gaaataaaag ttcgtgtgct gcatccattt
    44221 tgagcaaaat tccgaacaat tactttatca gtgaagtatt ccggcaacaa ttgaccccag
    44281 cctcgttcca aaaacttttc gtaagtaact tcgcctgtaa atgaatcggt tacgctttta
    44341 taaaacggtt tatttgccat tgtgctatcg ccagccataa aaatataaat gggacgattt
    44401 gtcaacgaaa gcaacaatgc acttaagcaa agaaggacaa aaattttact tttcattttc
    44461 atttatttgt agtttaatac attaaacatt atcaggactt tgtaaaaaag aagggaaagt
    44521 tttatttttg tattacaagg tttatattta tccttttgaa atgagttaca ttataagaga
    44581 taaacataac taaaatgcta cttatggtta ttcctggtta tgaagatttt agaaagataa
    44641 gatttcaatt ttttatctac tataaactta ttaattaatg aattttattt ttcagacaat
    44701 gtcaacatct tatcatttca ataaattcgt ctgcctaaaa tttttattaa acaaggattc
    44761 aaaaaacttt taatttttga aagcaagtca gttttaaatt attgcttaca aaaagttgta
    44821 ctttttatta tgttgttctg agatgattca aagatgcttt ttaatgttct tctttttcga
    44881 ttggcgaatt gtaaataccc tatatatacc agagaaaatt cacttttgaa atcttttttc
    44941 tatttcttct tctgaaacaa caatcatgat ttttttaccg tcgggtgtat aataaggatc
    45001 tttgcaagca aat
//
LOCUS       DAOSAS010000002        35768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_871_length_35768_cov_10.715594, whole genome shotgun sequence.
ACCESSION   DAOSAS010000002 DAOSAS010000000
VERSION     DAOSAS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 35768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35768
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_871_length_35768_cov_10.715594"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLR72_00130"
     CDS             <1..325
                     /locus_tag="PLR72_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35655.1"
                     /translation="GLKVVSRFRDDARLQYILPPVKTGKKGRPKTNGGKVDKEHLNMD
                     FFSLVEQENADFLIYIFSMKNIKINHHNALLLERFFSMFAINPNLLKNSQNVKELLLY
                     GTLYA"
     gene            complement(449..781)
                     /locus_tag="PLR72_00135"
     CDS             complement(449..781)
                     /locus_tag="PLR72_00135"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HOK35656.1"
                     /translation="MKKTWRWFGKKDPITLDMLRQIGVEGIVTSLYDGSSSGKIEKPE
                     QELIAFSKTRLLQSGEKETVKFKLTSKDFSSFDDSVSSWIVEPGKYTVKIGNSSLKIG
                     QKVAFNVD"
     gene            complement(799..3441)
                     /locus_tag="PLR72_00140"
     CDS             complement(799..3441)
                     /locus_tag="PLR72_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOK35657.1"
                     /translation="MKRTNLIFLFFIASIVFSFHLQAVEPYLNTALTFDERVNDLLSQ
                     LTLEEKVSLMLYDSPAIERLGIPAYNWWNECLHGVARSGLATVFPQAIGMAAMWDTDE
                     MFNIATAISDEARAKHHDYVSKGKRGIYQGLTFWSPNINIFRDPRWGRGMETYGEDPF
                     LTAEMAIPFIKGLQGDDPKYLKLIATAKHFVVHSGPEAVRHSFDVHPSEYDLAETYLP
                     HFKRAIQEAGVYSVMCAYQRLNGEPCCGSKYVEDILRNKWGLKGYIVSDCGAISDFYE
                     PDKHHVVNTPEEAAAMAVKAGTDLNCGDTYKHLVNAVKMNLISESDIDVSVKRLLMAR
                     MKLGMFDPDKEVKYAQIPISVVDCEKHRLLALEAARKSMVLLKNDKNLLPFTKNVRKV
                     AVIGPNVDNEEVLLGNYNGYPSMKITPLKGIKAKLPKATIKYALGCRWTDEFPYLTPV
                     SENCFYTDKNLKTKGLNAEYFDNSQWQGTPKHTTIDRKIDFVWETNAPFPDMKYDQFS
                     VRWRGVIVPPVSGIYAIGGEAFSGFKLTLDGKVLTFGQSAHHAKKGFENVKLEKGKPY
                     EIELEYVQNNTEYPYVRLLWDVPGRDLKKEALDLAKKSDLVVLCMGLSPSLEGEEMNV
                     NIDGFYKGDRTDIKLPQVQTELIQEIVKLGKPTVLVLLNGSALAINWENENIPAIVEA
                     WYPGQAGGTAIADVIFGDYNPAGRLPVTFYKDVHQIPAFENYDMKGKTYRYFEGDPLY
                     EFGYGLSYTTFKYHLNSIPTSVKPGENIPVSVNVMNVGKKDGEEVVQLYVSLPNSKLK
                     KPIRALQGFRRVYLKAGETKTIDFLLKPNQFAARNDENIPIIEAGKVLISVGGKQPDE
                     KSIAIEKVVQKSVEVIGDKFYVNE"
     gene            complement(3456..5318)
                     /locus_tag="PLR72_00145"
     CDS             complement(3456..5318)
                     /locus_tag="PLR72_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOK35658.1"
                     /translation="MYHIKKINIIIVFLIILHLTGFSKDLVSKYELQWEDSTIYTKSY
                     IVDNKNPNASDMNPGTPNLPLLSISKAVQLVKAGEKVVIHSGIYREAIILKNSGKSAR
                     KMISIEAAPGEKVIIKGSRELNVKWIQNLVATDEPLKKDVIYTWLKGIWACQLPDSLF
                     ENDYNPFQLQNIEPKEFDLMPWAWLVKNRAPYNLARGMLFQDGKRMMQIASIDDLRLV
                     AGSFYVAKANKILYIHPFEEKNPNNAFFELVVQSHLLRPAGVGFGYVRLSGLSFEHCA
                     NGFLRTSTGAVTTWGGHHWIIENNTFSQINSSALEFGYYAFENGDDNPLNQPRGGKEE
                     GNTIVRNNIIHDCGTAGIRSYYVINGLIENNTIYNCGWQNAENYWEVAGIKLLQTRNT
                     LIKGNHIYNITGGNGIWLDWDNRYSQIKENIIYNIQTIQGGIFIEASQYPNLIDHNFI
                     WNINGNGVLANDADEVIIINNLIGHTSNSLVRAEVATNRFLNGRALTTNRNKVINNIF
                     IDGGEPISLQGKENIVDYNLYIFTFLPKSFFLNKWIKQSGFDCNSSEIEAKVSFDSDL
                     LLFNFESNMEIPKMPCFELSFKNTEELKKVENCLVLPGPFFNLGHKNKILLKIF"
     gene            complement(5770..7464)
                     /locus_tag="PLR72_00150"
     CDS             complement(5770..7464)
                     /locus_tag="PLR72_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOK35659.1"
                     /translation="MKKIKNIMLLFIGLLSMASCNDFLDIKPTTFVSDALIWKDKKLI
                     DQFVANTYGSLVCGFNRNTQGWNQDWSAAFGGNFDAGTDDFDGKFDANVNQFNTGQIT
                     AQSTPFIDQIWMSNFNIIRKCNTLIEGVPKAENIVLTPDEKKYYEAEARFLRAFCYFD
                     LAKTFGRAPLITNPQQLNEDLLTPATDFEGLINFIKEECDTYAKDLPLTISPEMKGRA
                     TRGAFLALKARALLYLASPLNNPSNEKERWEAAANAADDVIKLGIYNLYTQGNEPYYN
                     LFFDKSATNREIIFERRFQYPEITHNIHMQWSLDPEGINKGSWNGLYPTQNLVDTYET
                     TDGKLISDPSSIYDNQNPYANRDSRFYQTLLYHGCWWEGTQILIHKNIQHPELSGNCV
                     PNDYRARCGYGLRKMIQEYNGPADDLYTGAFAQDNNWPYFRYAEVLLNYAEAKNEELS
                     APDNNVYNAVNEVRHRAGLPDLPEGLSQNEMRKRIHNERHVELCLEEQRFFDLRRWKD
                     AETLREPIRGMTIEYDENAQINHYKIVQIEERVFNEANYYLPIPQSEIEKNPNLIK"
     gene            complement(7477..10860)
                     /locus_tag="PLR72_00155"
     CDS             complement(7477..10860)
                     /locus_tag="PLR72_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK35660.1"
                     /translation="MRICFILLFVFSFQLMALNTKAQDAVIELNNNSLTLGQLINEIE
                     RQTDYLVVYSNREIDTNQEINLPNKSDKVSTYLNEAFKRTDIAYEFENNYIVLLKRSN
                     QNASTVANLIQAAQQSQQKEISGKITDSSGDPIPGATVMVKGTTIGTITDADGKFFLR
                     IPANAQTLQISFIGMKNQEIPIGERTTFNIQLEEETVALEEVVAIGYGVQKKVNLTGA
                     VASVRSDDILKAQSANTANTLVGQIPGLIAKQPTGEPGNDNSQLFIRGVATFRGSTAP
                     TFIIDGIERTYEDFARLDPHEIESLSVLKDAASAAVFGMRGANGVILVTTKRGRPQKI
                     QASYSGNISTQSPTKLPSFANSADYARMKNIYMNKEIYTPEEIEKFANGSDPENYPNT
                     NWYKETLTKNAIQQQHNITLNGGREDIAYFTSFGYLNQGGLWNNLNYDRYSLRANIDA
                     NITKSTKLSVDISGRVEHTHSSPTNSTGVFQQLVRNTPILLAKNKDGLLIVPDATHPN
                     IIALISKEAGYNNSRNNTVTPLIELEQNLSFITDGLKIKGVFSYEKNNYAQKLWSKSP
                     SLYVRDPLNKDNYIPQPHGSPNLYENTYDNEYIEWQGHVSYNKSFKNHSFSVLAMGLG
                     QKENYHNLWVQRNSFDSEVMDQINAGNTQGQQLGGYDEEHARVSFVGRINYSYANKYL
                     FEVNSRRDASENFSPEKRWGTFSSVSGGWVMSEENFFEDLRKEINFLKFRVSYGTLGN
                     DNTGGIAFPYYSRFDLYFPNGAHAGGLPNDLGDYIFGNLVTKGLQPGPIANPLAAWET
                     SKKTNVGMDWGLFNIINLSLDVFKENRFDILAQRGSEVPWSFGGILPLENIGKVVNKG
                     IELLLSFNQKIGQFHYNLGGNFTYARNKVLEMAEAAGTTKYMKHTGRPIYSYYGYKTD
                     GIFQSQAEIDAYAKQEVAGIDYVTKPGDIKYVDVDGNGVVNAGDMTYLGYGNVPEIVY
                     GINGSISYKNFDFSFLFQGTGHVQVYLNGGVIMPYYNDGNLPSLWIKNAWTEEKPNSR
                     YPRLEASNHNFPNTEFPPVQTYLYDASYLRLKNIEIGYNFNYKWLVNNHIENIRIYLS
                     GQNLLTFTKVPQIDPENLHSQGWSYPQMKSFNGGITIQF"
     gene            complement(11070..12032)
                     /locus_tag="PLR72_00160"
     CDS             complement(11070..12032)
                     /locus_tag="PLR72_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOK35661.1"
                     /translation="MDERLIKFLLGELNDSERLQLLKEIKEDESLKEEYIRLQNIYAL
                     THLVEHKNDEEKGRKSFQQFIKRVQTHYRHKTIANVCKIAAITIVLIVSTVFLTLYFV
                     KNDSYNVTNMLYVPAGQRAQLTLQDGTVVWLNAQSTLTYPAHFSTRSRKVSLKGEAFF
                     DVTKNKHKPFIVETQNFKVKVLGTQFNIYNYPLSNISQVALVEGSIKICSNKMNVVLK
                     PNEEFTLKGDKGVISKIDNPNHFLWKDGIYCFENERLLDIIEKLQLYYDVTIKVDDPD
                     IFNVRYTGKFRQRDGIDEILYIIQKIRHFNIVKDAEHNIITLTK"
     gene            complement(12100..12663)
                     /locus_tag="PLR72_00165"
     CDS             complement(12100..12663)
                     /locus_tag="PLR72_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOK35662.1"
                     /translation="MQNHQPPFNNFETIYISYYPKLKRFAQEYVIDEAEAENIVQDIF
                     LMLWKKKELFTVDFNIRAYLFTTVKNKCIDFLRHKIIMKNAFNRMQEEYELTLQMKFQ
                     SLEEFNEQIFNNADIEKIINDAIQSLPEKCRQIFVMNKIEGKKQKTIAEELHISVNTI
                     ENQMAIAYRKLKEILKDYFPLLLFFFL"
     gene            complement(12950..14815)
                     /locus_tag="PLR72_00170"
     CDS             complement(12950..14815)
                     /locus_tag="PLR72_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HOK35663.1"
                     /translation="MSKKTFNPADWKPVETIHESSQNPPKGKDTLHVSHPSLSTSPSD
                     IEQITRRIEQSAIDIAPNYADWRDLGFAIADALGESGRQYYHRLSRFYPNYSAAETDK
                     QFDNCLKAHGHGITIKTFFHLAKQAGIEISNLQIAKLPNCQIGNLTILEKSKSITLQN
                     ADEIKTDSAQSSFSEELEDIFLPTFPEEIYPLLPQFLQQITHYAISPEDADLLLLGSL
                     VVLSACMPNVYGIYGGMTVYPNLFLFVSAQASAGKGRLSLCRRLVEPIHRQLRELNKL
                     EYEEYKRQQTEYVANKKNPDVEEPQEPPLRMLFIPANSSATAVFQILNDNQGVGLMFE
                     TEGDTLAQTFKSEHGNYSDGFRKAFHHEKISYLRRKDREYVTLDVPKLSALLSGTPRQ
                     IQSLIPDAENGLFSRFIFYCMNIRLQWMDVFACNEDESLDQRFDRLGDSFFDFYGFLK
                     KAGNIRFSFSHTQQTTFNEYFEKVQQQYWELLGSDYLGSIRRLGLITFRLAMVLTVLR
                     IMDTGCFSHGLVCSDNDFYIVMEMVKVLLQHSAYVFRQLPQSNQTQPAANNPKMAFFE
                     TLPAQFDRSQYLAVASRLQIHESSADKQIARFCNAGLLIRQTHGNYVKGEGHFDQ"
     gene            complement(15281..15874)
                     /locus_tag="PLR72_00175"
     CDS             complement(15281..15874)
                     /locus_tag="PLR72_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BT4734/BF3469 family protein"
                     /protein_id="HOK35664.1"
                     /translation="MEIINNSQVEPSNWAASLFSFFKAPISNTTPYKSVCLRQVYNAI
                     KGDYYKERTQRLRAITVPRQARKFKAAHFDYCTFSGTFMVRNDQALIKHSGLLCIDFD
                     HLNNVETLFKRLLQDEYFDTQLLFRSPSGDGLKWVIPIDILQAPHDIFFTAVANYIAK
                     THGIVVDKSGRDLSRACFLPHDPNAYLNPEIINPHNL"
     gene            complement(15858..16100)
                     /locus_tag="PLR72_00180"
     CDS             complement(15858..16100)
                     /locus_tag="PLR72_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK35665.1"
                     /translation="MTDEEVNKLVEYSFLEDWMDNQDVMQTLHISPRTLQTLRSNGTL
                     PFSRIGNKLYYRRQDIERIQKDNYTMYKIRHYGNNK"
     gene            complement(16235..16582)
                     /locus_tag="PLR72_00185"
     CDS             complement(16235..16582)
                     /locus_tag="PLR72_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK35666.1"
                     /translation="MDKLSFEQLLQAVEILLDKVEKIETLLNETKRELPESDRWFNLQ
                     ELCTYLPDKPARQTVYGWIGQKLIPYHKKGKKLQFLKSEIDAWLKEGKRKTIAEIQAE
                     AEQYILAKKGGLK"
     gene            complement(17289..18533)
                     /locus_tag="PLR72_00190"
     CDS             complement(17289..18533)
                     /locus_tag="PLR72_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35667.1"
                     /translation="MIVKNIYESKSDFVKFSPSEKIVYEMVKKLYQKETETTERIKEN
                     SLPQTTLFNCVHTITSKLKEEEYPEAFIDEIWDEIKHIRGKRNDPIKNRPAGDFWGDD
                     YDFHVNRWYEATVIFACVYVIMAIDCPEKVDCLETIKEKCAYNENSTPYFEYFEKSLQ
                     DWKKNQERFFESSEIQKDLKENSTNHYSENEWIANEKHVSWEFDNFRKTLVGKSTDEK
                     FRDCLEEYQREQNMPHPNSLYLKLLDREIQYYQRHIAETNSPTIQVNDIISVLQENKF
                     SPAKAVQILEIINYILYARQYPEDICGNIDKKINLFKQNIQPDEEETETQKNAQKRTL
                     KVTTDVMIALLKEAGYVANNDNTKIARLISYLTGFSEEKIRQRFSEPNELTSYHKEEV
                     ENVNKILVDINSEISIKYNKQR"
     gene            18611..18766
                     /locus_tag="PLR72_00195"
     CDS             18611..18766
                     /locus_tag="PLR72_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35668.1"
                     /translation="MQFSNSLLAAVIENYLKKLGGYKRDRKVFRKMIFEVRKIHIYSK
                     RHRQKLG"
     gene            complement(19285..20415)
                     /locus_tag="PLR72_00200"
     CDS             complement(19285..20415)
                     /locus_tag="PLR72_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOK35669.1"
                     /translation="MSTTKVTLRKRELPSGKITLYLDFYPPVRNPFTMKMTRREYLGI
                     YIAKNPKTSLEKSINAEKLKQAEAIRAQRELSLINEQYGFMDKTKQKMDFLAYFKNKL
                     TEHDEKWRIVYEHFYKFVHGKCTFAEITVDLCNGFRTYLLSAKQLNHNHKALSQNSAS
                     GYWSTFRGLLAIAYKEKYIFENINEYLYKIETLDSRRQFLTFEEVQALYNTPCRETAL
                     KNASLFSCLTGLRFSDILALQWENFETYSDGGLCIRLRTKKTNAEVLNPISQEAYELC
                     GKKSTGLVFKGLKKSMLQNILPEWLNDAGINKHITFHSFRHTFATLLVSNGSEIYTVS
                     KMLTHKNVSTTQIYADLIDEKKRQAAEMIKIKPNEKDVDKSE"
     gene            complement(20420..21313)
                     /locus_tag="PLR72_00205"
     CDS             complement(20420..21313)
                     /locus_tag="PLR72_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK35670.1"
                     /translation="MERKFEFQKVCQYCGQIFTALNSTTKYCSKTCANRAGKEEKRLK
                     RLNEDSNHIKEKYRQNLLSKDLLSITQAAYLLGVSRPTIYKMIANNELFTYRFTERIV
                     RIKRTDLEQKQLPLSPVNTPISEIQKQKEKLISKKEALEMFGISDTWFYKKIKSADLS
                     PTFIDGKAFYSINMLKKLFIKQGQQNISEWYSLPEIMDKFKVSRQYIYDYTSDHKIPK
                     KRQGKGILISKYHWDKARGLDPLENENYYTVEQAIKKLGIGRSHLYDLIRSYKMPKIK
                     RGRNILIERESIDNLIKYKKL"
     gene            complement(21577..22968)
                     /gene="mnmE"
                     /locus_tag="PLR72_00210"
     CDS             complement(21577..22968)
                     /gene="mnmE"
                     /locus_tag="PLR72_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOK35671.1"
                     /translation="MNPTICAISTPPGSGGIAIIRLSGPEAISYANKIFMPKKESLDL
                     FQQQPYMVNFGSIVNKKNEIIDDVLVTVFRAPHSYTGEDVVEISCHGSIYIQQQILQI
                     LIEEGCSLAKPGEFTQRAFLNKKMDLSQAEAVADLIAATSAASHRIALTQMRGGISNE
                     LKKLRSQLLDFVSLIELELDFSEEDVEFADRSKLLRLADEIEKLMRKLSNSFKIGNAL
                     KNGIPVAIIGETNVGKSTLLNVLLNEEKAIVSDIHGTTRDAIEDSVNIEGITFRFIDT
                     AGIRETKDEIETLGIERTFQKIEQASIILWLIDCTQLSERIESLTEKIAKKVEKKKFI
                     LVFNKIDKLTEEERNVVWEFFEHYKGERIYISAKKQLNIEKLKKALVKAAQLPEIQPG
                     DVVISNVRHYEALQNALKAIQRVKQGLENGLSGDFISQDLRECIYFLGEITGEITNDE
                     ILGNIFSKFCIGK"
     gene            complement(22965..23540)
                     /locus_tag="PLR72_00215"
     CDS             complement(22965..23540)
                     /locus_tag="PLR72_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOK35672.1"
                     /translation="MITDKETYKYYLKSDLHAYQLSRITWLNFWKMDCLRFQCRLRKI
                     EYLFNVKKKNPFCKFYLLCLQILNHRLGTRLGLSIPKNVFGAGLCIVHYGTIVVSPFA
                     KIGKNCRIHPSTCIGEYNGAPTIGDNVYIGPGAKIFGNITIGNNVAIGANSVVNKNIP
                     DNVTIGGIPAKIISNKSSIDLGVFPENFFEK"
     gene            complement(23582..24451)
                     /locus_tag="PLR72_00220"
     CDS             complement(23582..24451)
                     /locus_tag="PLR72_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOK35673.1"
                     /translation="MKYFPPSELLINEDGSIFHLHIKPEQLADNVILVGDPGRVATVA
                     SYFDSQECSASNREFKSITGTYKSKRITVISTGIGTDNIDIVLNELDALANIDLEKRT
                     EKPEFRQLTIVRIGTSGAMQPEIPLGSFLISEKSLGFDGLLSFYAGRDKVCDLDFEEA
                     FKKHVDWNPKLASPYVVDNDKELIERISKDDMLKGVTISANGFYGPQGRVLRLDLHDS
                     HLNDKIESFRHNGYKITNFEMESSAIAGLSKLMGHKAMTVCCIIANRRIESANTNYQP
                     QMEKLIQLVLDRI"
     gene            complement(25124..25369)
                     /locus_tag="PLR72_00225"
     CDS             complement(25124..25369)
                     /locus_tag="PLR72_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35674.1"
                     /translation="MEAKLKAEKIIEALTALSLGKNPNIFSGEIFRKLADHPNFIAIR
                     DAYIEYLKNFTGEINTQEDVKRLFDFRMKIVELFSQE"
     gene            complement(25454..27202)
                     /gene="aspS"
                     /locus_tag="PLR72_00230"
     CDS             complement(25454..27202)
                     /gene="aspS"
                     /locus_tag="PLR72_00230"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOK35675.1"
                     /translation="MYRTHTCGELRLTNVGQKTTLAGWVQRIRKMGGMVFVDLRDRYG
                     ITQLVFNQQINENLWKEANELGREFVIQATGEIAERSNKNLNLPTGEIEILVEELNIL
                     NESKTPPFTIEDNTDGGEDLRMKYRYLDLRRNIIRQNLELRHQLTIETRRYLDQLGFI
                     EVETPALIASTPEGARDFVVPSRMNPGEFYALPQSPQLLKQLLMVAGFDRYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFVTQEDVLQTFEGLIKYLFKTLKNVDFIQPFPRMRWA
                     DAMKLYGSDKPDIRFGMTFVELKDVVQGHQFSIFDNGEYVGGICVTGCADYSRKQLDE
                     LTEFVKRPQIGAKGLIYIKYEKDGTFKSSIDKFFNNEDLKKIASQFQSQAGDLILILA
                     GEELKTQKALCELRLEMGERLGFRDKNVFAPLWVVDFPLLEWDDELQRYKAMHHPFTS
                     PKLEDIPLLETDPGAVRANAYDLVINGVELGGGSIRIHDSKLQDQMFKVLGFTEEKAQ
                     AQFGFLLNAFQYGAPPHGGLAFGLDRFVALLNGLDSIRDCIAFPKNNAGRDVMINAPA
                     PLDNSQLEELHLTLKL"
     gene            complement(27234..28106)
                     /gene="fabD"
                     /locus_tag="PLR72_00235"
     CDS             complement(27234..28106)
                     /gene="fabD"
                     /locus_tag="PLR72_00235"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOK35676.1"
                     /translation="MKAYVFPGQGAQFVGMGKDLYEQSEIAKKYFEKANEILGFRITD
                     LMFEGTDEDLRQTKVTQPAIFLHSVISAFVLEDNFKPDMVAGHSLGEFSALVAAQALT
                     FEDGLILVSKRAQAMQKACEMEESTMAAIIGLDDETVEEVCDSIKDEIVVPANYNCPG
                     QIVISGTIRGVEKACELLKEKGAKRALKLSVGGAFHSPLMESARIELSQAIHQTSFST
                     PICPVYQNVNAYPQTNPENIKQNLIAQLTAPVRWTQTIKNMINDGATEFIELGPGDVL
                     KGLIKKINPNVMVR"
     gene            28367..28993
                     /gene="rsmG"
                     /locus_tag="PLR72_00240"
     CDS             28367..28993
                     /gene="rsmG"
                     /locus_tag="PLR72_00240"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOK35677.1"
                     /translation="MDLILKYFPHLTPTQISQFEALYGLYSDWNQKINVISRKDIENL
                     YEHHVLPSLGIAQVIHFQKGTSILDVGTGGGFPGIPLAILFPEVNFTLIDSVGKKIKV
                     ASEISKAIALENTVFKQIRLEEEKGQYDFIVSRAVMTMNELVKQGSKNILKRQINALP
                     NGIICLKGGEISNEIRNYKNIAEIFPLSNYFKEEYFKTKKIIYMPVFK"
     gene            29017..29664
                     /locus_tag="PLR72_00245"
     CDS             29017..29664
                     /locus_tag="PLR72_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK35678.1"
                     /translation="MKIKKFVFNPFEENTYIVSDETKEAIVIDAGCLDKEEKRLLKEY
                     IADNQLIIKYILNTHLHIDHQFGNRFLLENYFIAPLAGKEDEFFLDTFREQTYFFGIK
                     VQEEPAPLGGYLIDNQKINFGNTSLQVFHVPGHSPGSMAFYFENEKAIFTGDVLFKGT
                     IGRTDFEKGDFDTIIRSIIQRLLPLPDETVVYSGHGPITTIGNEKKYNPYLQNYC"
     gene            complement(29655..30260)
                     /gene="nadD"
                     /locus_tag="PLR72_00250"
     CDS             complement(29655..30260)
                     /gene="nadD"
                     /locus_tag="PLR72_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOK35679.1"
                     /translation="MNTQTENTNKKIGLYFGSFNPVHLGHVNLAQYIIDNQYVDEVWF
                     VVSPHNPLKEDTELVDENIRLKMVELAIQNHPQLKVSDLEFSLPKPSYTINSLHAFSE
                     KYPENKFYLLIGSDNVLIFDQWKDYQKILEEYPVLVYPRKNFDASHLLSKYPQMKFLS
                     TPYYDISSSTIRQALKEQKDVDKWLHPAVYQFIIENKLYQQ"
     gene            complement(30273..30839)
                     /gene="gmk"
                     /locus_tag="PLR72_00255"
     CDS             complement(30273..30839)
                     /gene="gmk"
                     /locus_tag="PLR72_00255"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOK35680.1"
                     /translation="MAGKLIILSAPSGAGKSTLVHHLLNSNLNLEFSVSATSRAPRLN
                     EKEGIDYYFLTPEEFKQKIANGEFLEYEEVYPDCFYGTLRSEIDRITQKDKNVIFDVD
                     VKGGLNIKRQFGNQALAIFIAPPNMEALKERLLKRGTETPEMIQKRIEKAAYEMSFAP
                     QFDKIIINDDLEKAKKEIEETVRDFLNK"
     gene            complement(30939..31814)
                     /locus_tag="PLR72_00260"
     CDS             complement(30939..31814)
                     /locus_tag="PLR72_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOK35681.1"
                     /translation="MLQSMTGFGKATCEYNNKKIVIEVKSLNSKQLDVSIKIPNLYRE
                     KELEIRNEISQKLERGKIDFSLFIENAGKETATQFNQTIIEGYYQQINDLSEYLGINP
                     PNNWFDIFLRLPDSLKTEISELDENEWQAVKKAIQQAFDELKTFRMQEGKAVENVFYN
                     KISNIQQLLNEIAPFEAERIEKIKEKLKEELESISDKVDYDKNRMEQELIYYIEKLDI
                     NEEKSRLQNHLNYFIQVLEEEQAQGKKLGFIAQEIGREINTLGAKANHSEIQKIVVKM
                     KDELEQIKEQVLNVL"
     gene            complement(31909..33579)
                     /gene="recN"
                     /locus_tag="PLR72_00265"
     CDS             complement(31909..33579)
                     /gene="recN"
                     /locus_tag="PLR72_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018909717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOK35682.1"
                     /translation="MLKSLYISNFALISEININFESGFSVITGETGAGKSIILGALSL
                     LLGQRADSKLIKEGEEKCVIEAKFDIHDYQLEDFFEKNELDYNSSNCLLRREITVAGK
                     SRAFVNDTPVSLQVLRELGDKLIDIHSQYENLLLSHADYQLQVVDTMAQNEKIRENYR
                     ITFFEWKTLQNDFRQLQKEAEKQLAEFDYLQFQFQQLNDAHLSENEQTELEAEFQTLS
                     HTEEIKEELQKAYYFLNEEQMAISMIKNAINSLDHIKNFIPEGENWYQRLQSVEIETK
                     DIANEIISLEERIEFQPDRLQWIENRLNEIYALEKKFKVSTEKELISIRDNIKDQLEK
                     IESFDDELADLQKKINEVYEQLIQQANILRETRIKATESIENYLKQQLSQLGMPYIQF
                     KVEISETFDFTENGKDNVQFLFSANKNQSLQPVAQIASGGEISRLMLSIKSLVAHRAG
                     LPTIIFDEIDTGVSGEIAHKMGQIMHDMSKDMQVIAITHLPQIASKGEYHYRVYKDES
                     GEKAQTHIVPLNQEERVQEIAQMLSGENVTEVSLKNAEELLNINYFNN"
     gene            complement(33628..34530)
                     /locus_tag="PLR72_00270"
     CDS             complement(33628..34530)
                     /locus_tag="PLR72_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOK35683.1"
                     /translation="MKKIKFLETILFFSLFQFVSAQELNCNININSDLIQGTNKSVFT
                     TLQKSMYEFINNRKWTEMTFTPEERIECTMNITVKKVEGDNFTAEILVQSRRPVYNSS
                     YTTTLFNFKDNDFTFTYKEFDQLEWNPSVITSNLTAVLSYYAYIIIGYDMDSFSRLGG
                     TSAFQAAENIVNMAQGINLSGWKAFESPRNRYALVSNLLDEAFKPFRNYFYEYHRLGL
                     DMMVDNAANARAKIAEGLHVLRDVNRARPSAVVISSFLDAKTDELMNIFSKGTEKEKI
                     LVVEILSDVDPAQTAKYEQILNSK"
     gene            complement(34527..35732)
                     /gene="coaBC"
                     /locus_tag="PLR72_00275"
     CDS             complement(34527..35732)
                     /gene="coaBC"
                     /locus_tag="PLR72_00275"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOK35684.1"
                     /translation="MLKDKKILVGVTGSIAAYKTAQLIRLLVRQGAQVKVVMTPTAKE
                     FISPLTLATLAKNPILVDFFDPTSGNWNSHVDLGTWADTFLIAPATANTIAKMAAGIA
                     DNLLLTTYLSIRCPVFIAPAMDVNMFFNKITQENLYKLQQQGNFIIEPSSGELASGLE
                     GKGRMEEPEKIVDFLEDYFTPKNLTGKKILITAGPTYEKIDPVRFIGNFSSGKMGFAL
                     AEVCARQGAEVLLITGPVHLSIHHPKVQQINVTSAEEMYHAVTNHFKDMDGAILSAAV
                     ADFTPVEKSENKLKHENDNLMLELKPTPDIAASLGKMKNEKQFLIGFALETNNEEKNA
                     FDKLKKKDFDFIVLNSLNDEKAGFGYDTNKITIFYPSGEKKAFNLKPKKEVAEDIVNE
                     LIQMLNTEK"
BASE COUNT        11240 a         6272 c         5659 g        12597 t
ORIGIN      
        1 aggattaaaa gtagtttcac gattcagaga cgatgctcgt ttgcaatata tacttcctcc
       61 agtaaagaca ggcaaaaaag gacgtcctaa aaccaatgga ggaaaagtag ataaagagca
      121 tttgaacatg gactttttct ctctggttga acaagaaaac gctgactttc tgatttatat
      181 tttttccatg aaaaacatta aaatcaacca tcacaatgct ttattgctcg aaagattttt
      241 ttccatgttt gccataaatc caaacttgct taaaaatagc caaaatgtca aagaactttt
      301 attatatggc accttgtatg cttaaaaaat taacgaacta ttataaatag taatctgaca
      361 ataaatacct tcatttttga ttacgagtta atttttaaaa atcgaatttt ggggatagtg
      421 ctttgctaac cttttcagca ataatttttt aatcaacatt aaaagccact ttttgaccga
      481 tctttaatga agaattgcca attttaactg tatacttacc tggttcaaca atccaagaag
      541 aaacggaatc atcgaaagag gagaaatctt tggacgttag tttaaatttc acggtttctt
      601 tttcgcctga ctgaagtaaa cgagttttac tgaaagctat aagttcttgt tcgggttttt
      661 caatttttcc tgaagaagag ccatcataaa gtgacgttac aattccttct actccaattt
      721 gccgcagcat gtcgagcgtg atcggatcct ttttgccaaa ccatctccac gtttttttca
      781 tatcaaatta accaattttt attcattaac ataaaattta tcgccgatta cctcaacact
      841 tttttgaacg accttttcta tagcaatgga tttttcatcg ggttgctttc caccaacaga
      901 aatcagcacc ttgccggctt caatgatcgg aatgttttca tcattgcgag cggcaaactg
      961 attgggtttg agtagaaaat caattgtttt ggtttcgccg gctttcaaat aaactcttcg
     1021 aaatccctgc aatgctcgaa tcggcttttt gagcttactg ttgggcaaag atacatatag
     1081 ctgaaccact tcctcaccgt cttttttacc aacattcata acattcaccg aaacgggaat
     1141 attttctccc ggtttaaccg aggttggaat gctgtttaaa tgatatttaa acgttgtata
     1201 actcaatccg taaccaaatt cgtataacgg atcgccttcg aaataacgat acgtttttcc
     1261 tttcatgtca taattttcga aagccggtat ttggtgaaca tctttgtaaa aagttaccgg
     1321 taatcttccg gccggattgt aatcgccaaa aattacgtcg gcaatcgctg ttccgccggc
     1381 ttgacccgga taccaggcct caacaatggc gggtatgttc tcgttctccc aattaatggc
     1441 caaagcactc ccattgagta aaaccagcac ggtcggtttt ccgagtttaa caatttcctg
     1501 tatcaattcg gtttgaactt gcggcaattt aatatcagta cggtcgcctt tgtaaaatcc
     1561 atcgatattc acattcattt cttctccttc caatgaagga cttaatccca tacagagtac
     1621 caccaaatcc gattttttag ccaaatccag tgcttctttt ttcaaatctc ttcccggcac
     1681 atcccacaat aatcgaacat agggatattc ggtattgttt tgaacatatt ccaactcaat
     1741 ttcataaggt tttccttttt caagcttcac attttcaaat ccttttttgg catgatgcgc
     1801 actttgtccg aaagtcaaaa cttttccgtc caatgtcaac ttaaagccac taaacgcttc
     1861 acctccaata gcataaattc cacttaccgg tggaacaatc acacctcgcc accgaactga
     1921 aaattggtcg tacttcatat cgggaaatgg tgcattcgtt tcccatacaa agtcgatttt
     1981 tctatcaatc gtggtatgtt ttggcgttcc ttgccattgg gaattatcaa aatattcggc
     2041 attcaatcct tttgttttca gattcttatc ggtatagaaa caattttcgg aaacaggcgt
     2101 caaataagga aattcatccg tccatcggca acccaaagca tattttatag ttgctttcgg
     2161 caactttgct ttgattcctt tcaaaggagt tattttcatg gaaggatatc cgttgtaatt
     2221 tcccagcaaa acctcttcat tatctacatt gggtcctata actgccactt ttcgaacatt
     2281 ctttgtaaaa gggagcaaat ttttatcatt cttgagcagc accatcgatt ttcttgcagc
     2341 ttccaaagcc aacagccgat gtttttcgca atctaccaca ctaatcggaa tttgagcata
     2401 tttcacctct ttgtcggggt cgaacatgcc caacttcatg cgagccatca gcaatcgttt
     2461 tacggaaacg tcaatatccg attcgcttat caaattcatt tttacggcat tcaccaaatg
     2521 tttgtacgtg tcgccacagt tcaaatctgt accggctttt acggccattg ctgctgcttc
     2581 ttctggagta ttcactacat gatgcttgtc tggctcgtaa aaatcgctga ttgcaccaca
     2641 atccgacaca atgtatccct tcaatcccca tttgtttcgc aggatgtcct ccacatactt
     2701 gctgccacag caaggttcac cgttcagacg ctgataagca cacatcaccg agtaaacacc
     2761 agcttcttga atagcccgct tgaaatgggg cagataagtt tctgccagat catattcgct
     2821 gggatgcaca tcaaaggaat gacgaacagc ttccggacca ctgtgcacta caaaatgttt
     2881 cgccgtggca atcagtttca aatatttcgg atcgtcgccc tgcaaacctt tgataaacgg
     2941 aatagccatt tcagccgtca gaaaaggatc ttcgccgtag gtttccatac ctctacccca
     3001 acgtggatcg cgaaaaatat taatattggg actccagaaa gtcaatccct gataaatgcc
     3061 ccgcttccct ttcgacacat aatcgtgatg tttggcacgt gcttcgtcgg aaatggctgt
     3121 agcaatattg aacatttcgt cggtatccca catggcagcc atcccaatag cttgcgggaa
     3181 gacggttgcc aagcccgaac gtgctacgcc atgcaggcat tcgttccacc aattataagc
     3241 agggattccc aatcgctcga tggccggcga atcataaagc atcaaactca ccttttcttc
     3301 caacgttagt tgtgaaagca aatcgttcac tctttcatca aaagttaaag ctgtgttcaa
     3361 ataaggttca acggcctgta aatgaaacga aaaaacaata gatgcaataa aaaacagaaa
     3421 aatcaaattt gttcttttca caattaaaaa ataaattaaa aaatttttaa aagtatttta
     3481 ttcttatgac ctaagttaaa gaatgggccc ggcaatacta aacaattttc tacttttttt
     3541 aattcttcag tattcttaaa agaaagttca aaacaaggca ttttaggtat ttccatattg
     3601 ctctcaaaat tgaataataa taaatcagaa tcaaaactta cttttgcttc tatttctgaa
     3661 ctattgcaat caaatcctga ttgtttaatc cacttattta agaaaaaaga cttaggtaaa
     3721 aaagtaaaaa tataaagatt ataatccaca atattttctt taccttgtag agatattggt
     3781 tcccctccat caataaaaat attatttata actttatttc gatttgtagt taaagctctt
     3841 ccatttaaaa aacggtttgt ggcaacttca gctctaacaa gtgaatttga agtatgccct
     3901 attaaattat ttataataat tacttcatcc gcatcattag ccaaaacacc attcccatta
     3961 atattccaaa taaaattatg atcaatcaga ttaggatact gtgatgcttc aataaaaatt
     4021 cctccttgaa ttgtttgtat attgtaaata atattttctt ttatttgaga ataacgatta
     4081 tcccaatcca accaaatacc attacctcct gttatattat aaatatgatt tcctttaata
     4141 agggtattgc gagtttgtaa aagcttaatt cctgcaactt cccaataatt ttctgcattt
     4201 tgccatccac aattataaat tgtattattc tcaatgagtc catttataac ataataactt
     4261 cttataccgg cagtcccaca atcatgaata atattatttc gaacaattgt gttcccttct
     4321 tctttcccac ctcgaggttg atttaaagga ttatcatctc cattttcaaa agcataataa
     4381 ccaaattcaa gtgcagaaga attaatttgg ctaaaagtat tattttctat tatccaatga
     4441 tgccctcccc atgtggtaac tgcaccagta ctggtgcgta aaaaaccatt cgcacaatgt
     4501 tcaaatgata aacctgaaag tcgaacatac ccaaaaccga ctccagcagg tcttaataga
     4561 tgactttgga caactaattc aaaaaaagca ttatttggat ttttttcttc aaaaggatga
     4621 atatataaaa tcttatttgc ttttgcaaca taaaaagaac ctgctaccaa ccttagatca
     4681 tcaattgaag ctatttgcat cattcttttg ccatcttgga ataacattcc gcgagctaaa
     4741 ttataaggtg ctctattttt gactaaccaa gcccatggca ttagatcaaa ttctttgggt
     4801 tcaatatttt gaagttgaaa aggattataa tcattttcaa acaaagaatc aggaagctga
     4861 caagcccata ttcctttcaa ccacgtataa attacatctt tttttaaagg ttcatcagta
     4921 gcaactaaat tttgtatcca tttaacatta agttctctag atccttttat aatcaccttt
     4981 tctccggggg ctgcttcaat actaatcatt tttcttgctg attttcctga attcttcaga
     5041 attatcgctt cacgataaat acccgaatga attaccactt tttcgcctgc tttaaccaat
     5101 tgaactgctt ttgaaatact taaaagaggt aaattgggag ttcctggatt catatcagaa
     5161 gcattaggat tcttattatc tacgatataa cttttagtgt atattgtaga atcttcccac
     5221 tggagttcat attttgaaac tagatctttg gaaaagcctg ttaaatgcaa aataatcaaa
     5281 aatactatta taatatttat tttttttatg tgatacattg ttttgctttt gttattttac
     5341 ataaatttaa gtctactctg aaaaatcgat ttttaatcgg attttccttt ccaaattttg
     5401 taaaaaatga tctaaaagcc aataccaatc ggcttttaag aatattccag taaatttaat
     5461 gtacgcaaga aaaatactat tttttttctt ctaacaaagt tattttattg aatttttttg
     5521 tatatttgta aaaaataaaa taatgttcaa tatgtgataa ataactaata attacactgt
     5581 aagcaagaaa ttatttttaa aaaatatatt aaacaaataa taatcaattt gttatatata
     5641 aaatatacca ctttttgaaa ggaaaatccg tttaatattt acaaattttg aatattaata
     5701 ttcactctta aaaaaattaa aaggacagac ttcttagagt agactcatat gtttttgtac
     5761 tttttaaatt tattttataa gattaggatt tttttcaatt tcactttgag gtataggaag
     5821 atagtaattt gcttcattaa aaactcgttc ttcaatttgt actattttat agtgattaat
     5881 ttgcgcattc tcatcatatt caattgtcat ccctctaatt ggttcacgaa gtgtttcggc
     5941 atctttccaa cgacgaagat caaaaaaacg ctgttcttct aaacaaagct ctacatgcct
     6001 ttcattatga atacgttttc gcatctcgtt ttgtgaaaga ccttctggta aatctggtaa
     6061 acctgcacga tgcctcacct cattcacagc attatacaca ttgttatcag gtgcacttaa
     6121 ttcttcattt tttgcctcag catagttgag aagtacttcg gcatatcgaa agtatggcca
     6181 attattatcc tgagcaaaag ctccagtata caaatcatca gcaggaccat tatattcttg
     6241 aatcatttta cgtaaaccat aaccacatct tgctcgataa tcattgggaa cacaattacc
     6301 actcaattct ggatgttgaa tatttttatg aataagtatt tgagtacctt cccaccaaca
     6361 accatgatat aagagagttt gataaaaacg gctatcacga tttgcatagg gattttgatt
     6421 atcataaata gaagaaggat cactgattaa tttaccatcg gttgtttcat aagtatccac
     6481 caagttttga gtaggataca agccattcca tgacccttta tttatacctt ctggatcaag
     6541 actccattgc atatgaatat tatgtgtaat ttcaggatat tggaaacgac gttcaaagat
     6601 aatttctcta ttggtcgcac ttttatcaaa aaacaaatta taataaggct cattaccttg
     6661 tgtatataaa ttataaatcc ctaattttat aacatcatca gcagcattgg cagccgcttc
     6721 ccatcgttct ttttcattgg atggattatt taaagggctt gctaaataga gtaaagctcg
     6781 tgcttttaat gcaagaaaag ctcctcttgt tgctcgacct ttcatctctg gagatattgt
     6841 taaaggaaga tctttcgcat atgtatcgca ttcttcttta ataaaattga ttaaaccttc
     6901 aaaatccgtt gccggagtaa gaagatcttc atttaattgt tggggatttg taattaaagg
     6961 agcacgtcca aatgtttttg ctaaatcaaa gtagcaaaaa gccctaagaa atcttgcttc
     7021 tgcctcataa tatttttttt catcaggtgt taaaactata ttttctgctt taggcacccc
     7081 ttctattaga gtattgcact tacgaataat attaaaatta ctcatccaaa tttgatcaat
     7141 aaaaggagtg ctttgggcag tgatttgtcc agtattgaac tgatttacat tggcatcaaa
     7201 tttaccatca aaatcatctg tcccagcatc aaaattgcct ccaaaggcag cagaccaatc
     7261 ctggttccac ccttgtgtat tacgattaaa tccacaaacc aagctgccat atgtatttgc
     7321 tacgaactga tctattaatt ttttatcttt ccaaataagt gcatccgaaa caaaagtagt
     7381 aggtttaata tctaagaaat cattgcaact tgccatgctt aataaaccta tgaataataa
     7441 cataatattt tttatttttt tcatgatttt ttatttttaa aattgaatag taatacctcc
     7501 attaaatgat ttcatttgag gataagacca accttgagaa tgtaaatttt caggatctat
     7561 ttgaggtact tttgtaaaag tcaataaatt ctgtccactc aaataaatac ggatattttc
     7621 tatatgattg ttaacaagcc atttataatt gaagttataa ccaatttcaa tattttttaa
     7681 gcgtagataa gaagcgtcat ataaatatgt ttgaactggc ggaaattcgg tattcggaaa
     7741 attatgattt gaagcttcta atctggggta acgagaattt ggcttttctt cagtccaagc
     7801 attctttatc cataaggaag gtaaatttcc atcattataa taaggcataa ttacgccacc
     7861 atttaaatat acttgaacat gacctgtacc ttgaaataaa aaactgaaat caaaattttt
     7921 ataggaaata gagccattta tgccgtaaac aatttcagga acattaccat aaccgagata
     7981 agtcatgtca cctgcattta caaccccatt accatctaca tctacatact taatatcacc
     8041 tggtttagtt acataatcta ttcctgctac ttcttgttta gcatatgcat ctatttctgc
     8101 ttgtgactga aaaattccat cagttttata accataataa ctataaattg gtctccctgt
     8161 atgtttcata tatttagttg tgccagctgc ttctgccatt tccaatactt tatttcgcgc
     8221 ataagtaaaa tttcctccta agttatagtg gaattgacca attttttgat taaaagaaag
     8281 caataactct atccctttgt ttactacttt tccaatattc tctaaaggca aaattccacc
     8341 aaatgaccat ggaacttcac taccgcgttg agcaagaata tcaaaccgat tttctttgaa
     8401 tacatcaaga gacagattaa ttatattaaa aagtccccaa tccatcccaa cattagtttt
     8461 ctttgaagtt tcccaagctg ctaaaggatt agctattggt cctggttgaa gccccttggt
     8521 aactaggtta ccaaaaatat aatcacctaa gtcattgggt aaaccaccgg catgagcccc
     8581 atttggaaaa tacaaatcaa agcgactata ataaggaaaa gcaatgccgc cagtattatc
     8641 attacctaat gttccatatg atactctaaa tttaaggaaa tttatttctt ttcttaagtc
     8701 ttcaaaaaaa ttttcttcag acattaccca acctccagaa acagaactaa aagttcccca
     8761 tcgtttttca gggctaaaat tttctgaagc atctcttcgt gaattaactt caaaaagata
     8821 tttattagca taagaataat ttattcgtcc tacaaaactt actcgtgcat gttcttcgtc
     8881 ataaccacct aattgttgcc cttgtgtatt tccagcgtta atttgatcca ttacttcaga
     8941 atcgaatgaa ttacgttgaa cccataaatt atgataattt tctttttgcc ctagccccat
     9001 cgctaagact gaaaaagagt gatttttaaa agatttatta taactcacat gtccttgcca
     9061 ttcaatatat tcattatcat aagtattttc atataagtta ggactaccgt gtggttgtgg
     9121 aatataatta tctttattta aaggatctcg tacatataag gatggcgatt tagaccataa
     9181 tttttgagca taattatttt tttcataaga aaaaactcct tttattttta aaccatcagt
     9241 aataaaagaa agattttgtt ccaattcaat aagaggagta acagtattgt ttcttgaatt
     9301 attatatcca gcctctttac taattaacgc tattatattc ggatgagttg catcaggcac
     9361 aataagtaaa ccatccttat tttttgctaa caatattggc gtgttacgta caagttgttg
     9421 aaaaacacca gtgctgtttg ttggactact atgagtatgc tcaactcttc cagaaatatc
     9481 tacagataat ttggtggatt tagttatatt agcatcaata tttgctctta gtgaatagcg
     9541 atcataattt aaattattcc aaagtccgcc ttggtttaga tagccaaaac tagtaaagta
     9601 ggcaatatct tcacgtccac catttaaagt aatattatgc tgttgctgga ttgcattttt
     9661 cgtaagagtt tccttatacc aatttgtatt aggataattt tctgggtcgg agccatttgc
     9721 aaatttttca atttcttcgg gagtataaat ttctttattc atgtatatat tcttcattct
     9781 cgcataatct gcagaattgg caaagctagg aagcttagtc ggactttgag tagaaatatt
     9841 ccctgaatag gaagcttgaa ttttttgtgg tcttccacgt tttgtcgtca ctagaatcac
     9901 tccattagca ccacgcattc caaaaacagc tgcagatgca gcatctttta aaacacttaa
     9961 tgattcaatt tcatgtggat ctaaacgagc aaaatcttcg tatgttcttt ctatgccatc
    10021 aataatgaat gttggtgcgg tacttcctct aaaagtggca actccacgaa taaaaagttg
    10081 tgaattatca tttcctggtt ctccagtagg ttgttttgca atcaagcccg gtatttgtcc
    10141 aactaaagta ttagccgtat tagctgattg tgcttttaga atatcatcac ttctcactga
    10201 agcaacagct ccagtcaaat ttactttctt ttgtactcca taaccaattg ctaccacttc
    10261 ttccaaagca accgtttctt cttccaactg aatattaaac gtagttctct ctccaatggg
    10321 tatctcctgg tttttcattc caatgaaaga aatctggagt gtttgagcat tagcaggaat
    10381 acgaaggaaa aattttccat ctgcatctgt aattgtccca atagtagtac cttttaccat
    10441 tacggtagca cccggaatag gatcacccga cgaatctgtt atttttcccg aaatttcttt
    10501 ttgctgtgat tgttgagctg cttgaataag gttggcaact gttgaagcat tttgattgga
    10561 tcttttcaat aatactatat agttgttttc aaactcataa gctatatctg ttcttttaaa
    10621 agcttcattc aaataagtgg atactttgtc tgatttattt ggaagattta tttcttgatt
    10681 tgtgtctatt tcgcgattgc tataaactac caaataatca gtttgccgtt caatttcatt
    10741 gatcagttga cctaaggtta atgaattatt gtttaattca ataaccgcat cttgggcttt
    10801 agtatttaaa gccatcaact gaaaagagaa aacaaataaa agaataaaac agattctcat
    10861 aattctgaat atctctttaa aagtttcatt ttttattaaa taaagtactt ttagaaattt
    10921 ttttttcata tctttgaaaa attaaaagtg aatactaaaa aaagcttgta ggtgtttact
    10981 tttacgggcg atgagtgttg gcgcacttat cgcctttgtt ttatgatttt ctttttttca
    11041 taggcattaa attttaaagg ttaataaatc tatttagtta aagttattat attgtgctcg
    11101 gcatctttta caatattaaa atgacgaatt ttttggataa tgtacaaaat ttcatcaatt
    11161 ccatcacgtt gccggaattt tcctgtatat cttacattaa aaatatcagg atcatctact
    11221 ttaattgtaa catcgtagta aagttgtaat ttttcaatga tatccaacaa tctttcattc
    11281 tcaaaacaat aaatcccgtc tttccagaga aaatgattag gattatcaat tttagatata
    11341 actcctttgt caccttttaa agtaaattct tcattaggct ttaaaacaac attcatttta
    11401 ttactacaaa ttttgattga cccttccacc agagcaactt gtgaaatatt tgataaggga
    11461 taattataaa tattgaattg tgttccaagt acttttactt taaaattttg tgtttcgaca
    11521 atgaaaggtt tatgtttatt ttttgttaca tcaaaaaacg cttctccttt taaggagact
    11581 ttgcgtgatc tagttgaaaa atgtgcagga taagttaaag tggattgagc attcaaccat
    11641 acaactgttc catcctgaag agtgagctgc gctctttgtc cagctgggac ataaagcata
    11701 ttggtaacat tataagaatc atttttaaca aaatataaag ttaaaaaaac agtagataca
    11761 ataagaacta tcgtgattgc agcaatttta catacatttg caattgtttt gtgacgatag
    11821 tgagtttgca ctcttttaat aaattgttga aaactttttc ttcctttttc ttcatcattt
    11881 ttatgctcaa ctagatgcgt taaagcataa atattttgta aacgaatata ttcttctttt
    11941 aatgattcgt cctcttttat ctctttcaat aactgtaaac gttctgaatc attaagttca
    12001 cccaataaaa attttataag tctttcatcc attatttatt attatataac tattaaacgt
    12061 ttgaaaagaa aaaatccact aaaaaatatc gaaatatttt taaaggaaaa agaaaagtaa
    12121 taaagggaaa taatctttta aaatttcttt taatttcctg taagcgattg ccatttgatt
    12181 ttcaatagtg ttgaccgaaa tatgaagttc ttcagcaatc gttttttgtt ttttgccttc
    12241 tatcttgttc attacaaata tttgtcggca tttttcaggt aaagattgaa tggcatcatt
    12301 aataattttt tcaatatcag cattattgaa aatctgttca ttaaattctt ctaaagactg
    12361 gaatttcatt tgtaaagtta attcatattc ttcctgcatt ctgttgaaag cattcttcat
    12421 tattattttg tgacgcaaaa aatcaataca tttatttttt acagtagtga aaagataagc
    12481 tcttatgtta aaatcaactg taaaaagttc ttttttcttc cataacataa gaaatatatc
    12541 ttggacaatg ttttcagctt ctgcttcatc aatcacatat tcttgggcaa aacgctttaa
    12601 ttttgggtaa taagaaatat agattgtttc aaagttgtta aaaggtggtt ggtggttttg
    12661 catggtttat ttcctaattt tatagaatta gcctcaaata tatgaaaatt ttataaattt
    12721 agcagcgttg gaaaattatt gtttgttttt gattcctaat attcttcaag aataaaatct
    12781 ttcaaaatat agattttctt tatgtttagt actagttcag tactcgacat aaaatattct
    12841 tatagacttt aattttatta cttgtcttaa ttttttaatt aaaagttaga taagaatatt
    12901 gactatttag tcaatcaacg attataatgg gaacaaccta attattgaat cattgatcaa
    12961 agtgaccctc tcctttcacg tagttgccat gagtttgacg gattaggagg ccggcattgc
    13021 aaaagcgggc tatctgcttg tcggcagagc tttcgtggat ttgcaaacgg gaggctactg
    13081 caagatactg agaacggtcg aattgagcgg gtaatgtttc aaaaaatgcc attttagggt
    13141 tattagctgc tggttgtgtc tgattggatt gtggaagttg tcgaaaaaca taagcggagt
    13201 gctgaaggag tacttttacc atttccatga caatataaaa atcgttgtcg gaacaaacca
    13261 acccatggct gaaacaacct gtatccataa tacgtaaaac ggttaagacc attgccaaac
    13321 gaaaagtaat caaacctaaa cgacgaatag aacccaaata atcgctgccc aatagttccc
    13381 aatattgttg ctggactttt tcgaagtatt cattaaaggt tgtttgctga gtatgcgaaa
    13441 aagaaaaacg aatgttacct gcttttttca ggaagccata aaagtcgaaa aaactatcac
    13501 ctaaacgatc gaaacgctga tctaacgatt cgtcttcgtt gcaagcaaac acatccatcc
    13561 attgtaagcg aatattcatg cagtagaaga taaaacgact gaacaagcca ttttcggcat
    13621 caggtatcag gctttgaatc tgtcgaggtg tgccggagag cagggcagaa agtttgggta
    13681 catcgagggt aacatattcc cgatccttgc gtcgcaaata agagattttt tcgtggtgga
    13741 acgctttgcg aaacccatcg gaatagttgc cgtgttcact tttgaaagtt tgcgccaatg
    13801 tatccccttc cgtttcgaac attaaaccaa ccccttgatt atcgttgagg atttgaaaaa
    13861 cggcagtggc actgctattg gctggtataa agagcatccg taagggtggt tcctgcggct
    13921 cttcgacatc aggatttttc ttgttagcta catattccgt ttgttgacgc ttgtactctt
    13981 catattcgag cttgtttaat tcccgcaact gtcggtgaat aggttcaaca agacgacggc
    14041 aaagcgataa acgacctttg ccagcagaag cttgagcaga aacaaagagg aacagattcg
    14101 gataaaccgt catcccgcca taaatgccat aaacgttggg catgcaagct gagaggacaa
    14161 ccagcgaacc caataaaagg aggtctgcat cttctggaga aatggcataa tgagtaattt
    14221 gctgtaaaaa ttggggtaaa aggggataaa tttcttccgg aaaagtaggt aaaaaaatat
    14281 cctctaactc ttctgaaaag gaagattgag cactatccgt tttgatttcg tcagcatttt
    14341 gcaatgttat tgatttagat ttttccaata ttgtcaaatt tccaatttgg caatttggca
    14401 atttggcaat ttggagattt gaaatttcaa taccggcctg ttttgccaag tggaaaaatg
    14461 ttttgatggt tatgccgtgt ccgtgagctt tgaggcaatt gtcgaattgt ttgtcggttt
    14521 cggcagccga atagttggga taaaaacggc tcaatcggtg ataatattgt cgtccacttt
    14581 cgcctaatgc atcagcaatg gcaaagccaa ggtcacgcca atcggcataa ttgggggcga
    14641 tgtcgatggc ggattgttca atacgacggg tgatttgttc gatatcggat ggagaggtgc
    14701 ttaaagaagg atgagacaca tgcaatgtgt ctttaccttt tggcggattt tgagacgatt
    14761 cgtgaattgt ttcaacaggt ttccaatcgg ctggattaaa tgttttcttg ctcattgtta
    14821 tgggaattat agaaattttt aaattgaatc aattgattat tgggaaatgg ggcgttgccc
    14881 caaaacttca ctttaatatg agccgttaca tgtaacggct ctactaaaaa aaggacgttt
    14941 gttggctaaa atcttttaaa ctcgctcctt cggagctcaa acagaaaaga tttttttaac
    15001 gccaacttct cttatttttc ggctcaccgg ctcatgtcat tccataaatt atagtttctt
    15061 ggtacattca tttcttcaac tttattttgc taaaaactta ataacaagta aactccttcc
    15121 gcaatccaaa aacttattaa cttattaaaa aatctggaga ttttgttttt gcttgtgacg
    15181 tagcctatag gctacgccga acgggagatt ttgagacgat tcgtgaatcg tctctacagg
    15241 tttccaatcg gcgggattaa atatttgctt gctcattatt ttataaatta tggggattaa
    15301 ttatttccgg gttgagatag gcgttgggat cgtggggaag aaaacaggcg cgggataggt
    15361 cgcgtcccga tttatcgacg acaatgccgt gggttttggc aatataattg gcaacggcag
    15421 tgaagaaaat gtcgtgcggt gcctggagga tgtcaatggg gatgacccac tttagaccat
    15481 cgcccgaagg acttcgaaaa aggagttgcg tgtcgaaata ttcgtcttgt aaaaggcgtt
    15541 taaataatgt ttcaacgttg tttaaatggt cgaagtctat acaaagcaag ccggaatgtt
    15601 tgattaaagc ctggtcatta cgaaccataa acgttcctga aaaggtgcag taatcgaaat
    15661 gggcagcttt gaattttctt gcctgcctgg gaacagtgat tgctcgcaat cgctgtgtcc
    15721 tttctttgta ataatcccct ttgatggcat tgtaaacttg ccttaaacaa accgatttgt
    15781 atggtgtggt gttgctaatg ggggctttga agaaagagaa caaggaagct gcccaatttg
    15841 acggttctac ttgtgaatta tttattattt ccataatggc gaatcttgta cattgtgtaa
    15901 ttatctttct gtatacgttc aatatcctga cggcgataat agagtttgtt accaattcga
    15961 gagaatggca aagtgccatt ggaacgcaag gtttgtaaag tacgaggact aatatgcaaa
    16021 gtttgcatga catcctgatt atccatccaa tcttcgagga aagaatactc aactaactta
    16081 ttcacttctt cgtctgtcaa attgtcgaga gaaatctgtt tttgtttcaa tcgttcagct
    16141 tgaatgcagg gacatttgcg gaacagtttg ccgaaaggtt cgttttgatt catagccatt
    16201 tcaaccagaa tttctgataa tcgtttgaga tctttcattt caaacctcct ttctttgcga
    16261 gaatatactg ttcggcttca gcttgaattt cagctatggt ttttcttttg ccttctttca
    16321 gccaggcatc gatttcgctt ttaagaaatt gaagtttttt tccttttttg tgataaggaa
    16381 tgagtttttg tccaatccat ccatagacgg tttgtcgggc aggtttgtcc ggtaaatagg
    16441 tacacaattc ctgtaagttg aaccaacggt cagattcagg aagttccctc ttcgtttcat
    16501 tcaacaaggt ttcaattttt tctaccttgt cgaggaggat ttccacagct tgtagaagtt
    16561 gttcgaaaga taatttatcc atattctttt attacccgtc tgattcagct gctcccttat
    16621 cgacggataa taagaaagcc ccaattctca tggaactgag gctacaaaag aataaacaaa
    16681 agaagggtgt aacgggtcac tgggacgggt tataaattat cgttacttat tgtatttaat
    16741 tgattatgtg agccattgca tgcaacggct ctactgaaat aatggatgtt cgttggctaa
    16801 aatcttttaa actcgctcct tacagagctc aaacagaaaa gattttttaa cgccaacttc
    16861 tcttattttt cggctcaccg gctcatgtca agactaaaaa ataagattgc cctgtggctt
    16921 tatttccttt aaccttattt ttactcaaac cttaataata gggaaactta ttcttattaa
    16981 ccgtattctc ttttcaaaaa tctggagatt ttgtttttag ctgcgacgta gcctggaggc
    17041 tacgccgaac ggggagtaca ggtgaatggc tgacgccagg gagaaaatct ggagatttta
    17101 tttttactta cgacgtagcc tggaggctac gccgaacaaa aggaaattaa caatttaaat
    17161 tggacatttt gccaaattgc caaattgcca ttttggagat ttggagaatt aatttttata
    17221 tgtggaaaat ttttagtgag atgtaggcta aaaaatatgc cgaacggatc actgggacgg
    17281 gttataaatt atcgttgctt attgtattta attgatattt cactatttat gtctacaaga
    17341 attttgttga cattttcgac ttcctccttg tgataagaag ttaattcatt aggttctgaa
    17401 aaacgttgtc ggattttttc ttctgaaaaa ccggtaagat aactaattaa tcgtgctatt
    17461 ttggtattgt cattgtttgc cacataacct gcttctttta aaagagcaat catgacatcg
    17521 gttgtaacct ttaatgttcg tttttgagca ttcttctggg tttcagtttc ttcttcatca
    17581 ggttgaatat tttgtttgaa caagtttatt tttttatcga tatttccaca aatatcttca
    17641 ggatattgtc tggcatatag aatgtaattg attatttcca atatttgaac tgcctttgca
    17701 ggagaaaatt tattttcttg cagtacggaa attatatcgt tgacttgaat ggtcgggctg
    17761 tttgtttcag caatatgacg ttgataatat tgaatctcac gatccagtaa tttcaaatat
    17821 agactgttgg ggtgtggcat attttgctcc cgttgatatt cttccaggca atctctaaac
    17881 ttttcatctg ttgattttcc aaccaatgtt tttctaaaat tatcaaattc ccaggaaaca
    17941 tgtttttcat tggctatcca ttcgttttca gaataatggt tagtagaatt ttcttttaaa
    18001 tctttctgta tttcagaaga ttcaaaaaat ctttcctgat ttttcttcca atcctgaagt
    18061 gatttctcaa aatattcaaa gtatggagta gagttttcat tataagcaca tttttcctta
    18121 atggtttcca gacaatccac tttctcggga caatcgatag ccataatcac atacacacaa
    18181 gcaaaaatta ccgttgcctc ataccatcgg ttgacatgaa aatcgtaatc atccccccaa
    18241 aaatctcctg ctggtctatt ttttatagga tcattccgtt tccctctaat atgtttgatt
    18301 tcatcccaaa tttcatcaat aaatgcttcc ggatattctt cctctttcaa ttttgaggta
    18361 atggtgtgaa cacagttgaa aagcgtagtt tgtggaagag aattttcttt tattcgttct
    18421 gttgtttctg tttctttttg ataaagcttt ttaaccattt cataaacaat tttttcactg
    18481 ggactaaatt tgacaaaatc acttttcgac tcataaatgt tttttacgat catggattat
    18541 ttttttaaag ttaatgttta atgtaattgt ttgaactaat agtaatttta tgtgtattaa
    18601 aaagagcaaa atgcagtttt ctaattcgtt gttagccgct gttatcgaaa attatttgaa
    18661 gaaattggga ggttataaac gggataggaa agtttttagg aaaatgatat ttgaagtaag
    18721 gaagattcat atttacagta aacgtcacag acaaaagtta ggttaaaagt ttttatcagt
    18781 tgaataaaaa tggttttata attggaatga aacaccaatt tttttgaaat ctaaattttc
    18841 agctaaaaag tgaaagagcc attgaatcca atagctcttc cgttagatat ttctatcaat
    18901 aaaaattgat aaattattcc tcaaaacagt gttctattct gttgggattt tcaaagacaa
    18961 aaatagaaat aataaacaaa actgaaaaaa gagagtagaa ttttgagtct gttttttaac
    19021 ttatatactg aaattattga tcccgatttt gccaaattgt caaattgcca ttttggagat
    19081 ttggcaattt ggagatttgg agatttggca atttggggat attaaaattg gcttgtgatt
    19141 tcatttattc acattatttt ctttaatatc aagatttaaa tcgagtttaa tggcattggc
    19201 aatgggcact acaaaaattt tattattctc tgtaatgaaa atttttctta tttgcgatta
    19261 ttcattgtcc agcatatttg tagattattc actcttatca acatcttttt catttggctt
    19321 aattttaatc atttctgctg cctgacgctt tttttcatca attaaatcgg cataaatttg
    19381 tgttgtagaa acgtttttat gagtcaacat tttgctaact gtgtaaattt cacttccgtt
    19441 tgaaacgagc aaagtggcaa aagtgtgtcg aaaagaatga aaggtgatat gtttattaat
    19501 tcctgcatca ttgagccatt ctggcaaaat attttgtaac atggattttt taagaccttt
    19561 gaagaccaga cctgtagatt tttttccaca taattcgtaa gcttcttgac tgattggatt
    19621 caagacttca gcatttgttt tctttgttct taatcttata caaagtccac cgtcagaata
    19681 tgtttcaaaa ttttcccatt gcaaagcaag tatatcactg aaacgtaacc cagtaagaca
    19741 agaaaagagt gaagcatttt tgagtgcagt ttcccgacat ggagtattgt aaagtgcttg
    19801 tacctcttca aaagttaaga attggcgacg ggaatctaaa gtttcgattt tgtaaaggta
    19861 ttcattgata ttttcaaaaa tatatttttc tttgtaagca atagccaaca ggccacgaaa
    19921 tgttgaccaa taacctgaag cagaattttg tgaaagagct ttgtggttgt gattaagttg
    19981 cttggcactc aaaagataag tgcgaaaacc attacacaaa tccactgtaa tttctgcaaa
    20041 agtacatttg ccatgaacaa atttgtaaaa atgttcgtaa acgattctcc atttttcatc
    20101 atgctctgtt aatttgtttt tgaaataagc caaaaaatcc attttttgtt tggtcttatc
    20161 catgaaacca tattgttcat taatcaatga caattctcgt tgagctcgta tagcttctgc
    20221 ctgtttcaat ttttcggcat taatgctctt ttcaagggat gttttcggat tttttgcaat
    20281 ataaattccc agatattcgc ggcgagtcat tttcatggta aatgggttac gaaccggagg
    20341 ataaaaatca agataaagcg taattttccc gcttggcaat tctcgtttgc gtaaggttac
    20401 ttttgttgtt gacattgagt tataattttt tgtattttat gagattatca atactttcac
    20461 gttctatcaa tatgtttctt cctcgtttaa tttttggcat tttgtatgaa cgaattaaat
    20521 catataaatg acttcttccg atacctaatt tttttattgc ctgttcgaca gtataataat
    20581 tttcattttc gagcggatct aaccctctgg ctttgtccca atgatatttt gaaattaata
    20641 ttccctttcc ttgtcttttc ttaggtattt tgtgatcact ggtatagtcg taaatatatt
    20701 ggcgggaaac tttaaattta tccataattt caggcaaaga ataccattct gatatatttt
    20761 gttgtccttg ttttatgaaa agttttttga gcatatttat tgaataaaaa gctttgccgt
    20821 caatgaaagt gggagagagg tctgccgatt tgattttctt gtagaaccaa gtatctgaaa
    20881 ttccaaacat ttcaagggct tcttttttac tgattaattt ttctttttgt ttttgtattt
    20941 cagaaatggg tgtattgaca ggtgaaagtg ggagttgctt ttgttccaag tctgtgcgtt
    21001 taattcttac aattctttct gtaaatcgat aagtaaataa ttcattattt gcaatcattt
    21061 tgtaaatcgt tggacgtgaa acgcctaaaa gatatgctgc ctgggttatg gaaagcaaat
    21121 cttttgaaag caaattttga cgatattttt ctttaatatg gttgctgtct tcatttaaac
    21181 gttttaagcg tttttcttct ttaccggcac ggtttgcaca agttttacta caatatttag
    21241 tagtactatt cagggcagtg aatatttgcc cgcaatattg acagactttc tgaaattcaa
    21301 attttcgttc cattatttat tttgctgata tttcgttatt attagagttt atttattgta
    21361 aattggcgta aatttatgta aattgacgta aaaatactgt atcatttagt ataagtattg
    21421 tgagatttat atttatatgg tacaaatgtg gtacaaagat atacaaaata tctgtaaaaa
    21481 agaataaata aaggaagaaa attattgaag gaataaatta taaataactg tatttcaata
    21541 gtttatttat tcttgattga ttgttgttag gagggactat tttccgatac aaaacttact
    21601 aaaaatatta ccgaggattt cgtcattagt aatttcacct gttatttcgc ccagaaaata
    21661 aatgcattct cgtaaatctt gtgaaataaa atcacctgac agcccatttt caaggccttg
    21721 ttttactctt tggatagctt ttaaagcatt ttgtaaagct tcgtaatgtc tcacgttgct
    21781 gattaccaca tcaccaggct gaatttcagg taattgagct gctttaacga gtgctttttt
    21841 taatttttca atgtttaatt gttttttagc agaaatataa attcgttcac ctttgtaatg
    21901 ttcaaaaaac tcccaaacca cattccgttc ttcttctgta agtttgtcta ttttattgaa
    21961 taccagaata aattttttct tttcaacttt tttagcaatt ttttcggtca atgattcgat
    22021 acgttcactc aattgtgtgc aatcaatgag ccataaaata atagaagctt gctcaatttt
    22081 ctgaaaagtt cgttctatgc caagcgtttc tatttcgtct tttgtttcac gtatgccggc
    22141 tgtgtcaata aaacgaaagg taattccttc aatgttcacc gaatcttcga ttgcatctcg
    22201 agttgtgccg tgtatatcag aaacaatggc tttttcttcg tttagtaaaa cattcaataa
    22261 tgttgatttt cccacattgg tttcacctat aatagccaca ggaattccat tttttaaagc
    22321 gtttccaatt ttaaaggaat ttgataattt tctcatcaat ttttcaattt cgtctgcaag
    22381 tctcaataat tttgatcggt cagcaaattc cacatcttct tctgaaaaat cgagttctaa
    22441 ttcaatcaat gaaacaaaat caagaagctg tgaacgaagt ttctttaatt cattggaaat
    22501 gccacctctc atttgagtta atgctattcg atgcgaagca gcagaagtgg cagcaattaa
    22561 atcggcaact gcttcggctt gagacaaatc cattttcttg ttcagaaaag ctctttgcgt
    22621 aaattctcca ggttttgcca gcgaacaacc ttcctcaatc aaaatttgca gaatttgttg
    22681 ttgaatataa atagatccat gacaagaaat ttccacaaca tcttcacccg tgtaagaatg
    22741 aggagcacga aaaacagtga ctaaaacatc atcaataatt tcattttttt tgtttactat
    22801 actaccaaaa ttaaccatat aaggctgttg ttgaaaaaga tcaagcgatt cttttttcgg
    22861 catgaaaatt ttattggcat aactgatcgc ttctggacca gaaagtcgaa taatagcaat
    22921 acctcctgaa ccaggtggtg ttgatatagc acaaatagtt ggattcattt ttcaaaaaaa
    22981 ttttcaggga aaacacctaa atctattgac gatttatttg atatgatttt tgcaggtatg
    23041 cctccgattg ttacgttatc gggtatattt ttatttacaa cagaattggc tcctatggca
    23101 acgttatttc ctatggttat gttaccgaaa atttttgctc ctggtcctat gtatacgttg
    23161 tcacctatag ttggagctcc attgtattcc cctatacagg tggaaggatg aattcgacaa
    23221 tttttaccta ttttggcaaa tggactcact acaatagtac cataatgaac aatacaaagc
    23281 cctgctccaa aaacattttt tggaatggat aaacctaatc ttgtccctaa acgatggttg
    23341 agtatctgca ggcaaagtaa gtagaattta caaaaagggt ttttcttttt tacattgaaa
    23401 agatattcaa tttttctcaa tcgacattga aatctcaggc agtccatttt ccaaaagtta
    23461 agccatgtta tacgtgacaa ttgataggca tgcaaatcac ttttgagata atacttgtat
    23521 gtttctttat ctgtaatcat ttttgcaatg ttgaggatca aagcaaatta ttcacattct
    23581 tttaaattct atccagtacc aattgaatca atttttccat ttgaggttga taattcgtgt
    23641 tggcagattc aattcttcga ttagctatga tacaacaaac agtcattgct ttgtgaccca
    23701 ttaatttcga tagtccagca atggcagaac tttccatttc aaaattagta attttatagc
    23761 cattatgtcg aaaactttca attttatcat tcaaatgact atcatgcaag tcaaggcgaa
    23821 gaacacgtcc ctgaggtcca taaaatccat tggctgaaat ggttactcct ttcagcatat
    23881 catctttact aattctttca attaattctt tatcattgtc aaccacataa ggagaagcta
    23941 attttggatt ccaatctaca tgttttttga aagcctcttc aaaatccaga tcacaaactt
    24001 tatctctacc agcataaaaa cttaaaagac catcaaatcc caacgatttt tctgaaatta
    24061 aaaaacttcc cagtggaatt tcaggttgca ttgctcctga agtgccaata cgaacaatgg
    24121 tcaattgtct gaattctggt ttttcagttc ttttttccaa atcaatatta gcaagagcat
    24181 ccaactcgtt caaaacaata tcaatgttat cggtaccaat tccagtggaa atcaccgtaa
    24241 ttcgtttgga tttgtaagta cctgtaatgc ttttaaattc acgattcgat gctgaacatt
    24301 cttgtgaatc aaaatatgaa gcaactgtag caactcgtcc aggatcacca accaatatta
    24361 cattgtcagc caactgttca ggtttgatat gcaagtgaaa aatgctacca tcttcattga
    24421 taagcagctc ggaaggtggg aaatatttca taacggtaaa aaattaatgt atttttttaa
    24481 atatagattt gcttaattgc cacaaatata acctttttta ttcaattttt gctttttaat
    24541 ggctatttaa gctcttttta taaaaaataa ctcaaaaata tttttaaatg attgaaatac
    24601 aacaatttaa ggtattattc aaaataaaag agtaggggag gggtaattat ttaaaaatca
    24661 agtgataata tgagattaat aatcaactaa ataaagtaat aaggttataa tcaatttaat
    24721 cgaaagaatt cctcgaggct tgcctcgggg ttattcatta ataaaatttt aataacaaaa
    24781 ggttttaagg tcaaattgaa aatgaataaa attatcaaaa ttttttgcgg gtacaaaatc
    24841 acactaaaaa ctttattgtc ttattatatt ttgaattttt atcacaaaat atttgttgtt
    24901 tattaaacat gaccaaagaa caagaaaaaa tatacagtta gaggtatata ccttcgccag
    24961 tgtaacttta atgtaaattt aataaaattg aaagcatcac ataaaaaagg agaatgtcac
    25021 cgaaataaat tttctacata gttttcctat aatttatatt atgtcaaata gtataataat
    25081 ataaagctat ttaaattatc tttcttccct aattttcaat gcttcattct tgcgaaaata
    25141 actcaacaat tttcattcta aaatcaaaaa gccgtttgac atcctcttgt gtattaattt
    25201 cgcctgtaaa atttttgaga tattcaatat aagcgtcacg aatagctata aaattaggat
    25261 gatcagctaa ttttctgaaa atttcaccag aaaaaatatt aggattttta ccaagactta
    25321 aagctgtcag tgcttcaata attttttcag ctttaagttt tgcttccatt tcgttcatgt
    25381 tgttaataga ctttaaaaag ttaacaatgc tgcaaaattc agctaattac ttgtttatca
    25441 agcaataaga aaattataat tttaaagtta aatgtaattc ttccagttga ctattgtcta
    25501 aaggtgctgg agcattgatc attacatctc ttccagcatt atttttaggg aaagcaatac
    25561 aatcgcgaat agaatccaat ccatttaata aagcaacgaa tctgtccaat ccaaaagcca
    25621 gtccaccatg tggaggagct ccgtattgaa aagcattcag taaaaagcca aattgagctt
    25681 gagctttttc ttcggtgaaa ccaagcactt taaacatttg atcttgtaat ttactgtcgt
    25741 gaatacgaat agaaccacct cccaattcta caccatttat taccaggtca taagcatttg
    25801 ctcttactgc tcctggatca gtttccaaca aaggtatatc ttccaatttt ggtgaggtaa
    25861 aaggatgatg catagcctta taacgctgta attcatcatc ccattctagt aaaggaaaat
    25921 ctactaccca caaaggagca aatacatttt tatcacgaaa gcctaacctc tctcccattt
    25981 ccaaacgcaa ttcgcaaaga gctttttgtg ttttcaattc ttcgcctgcc agaataagaa
    26041 taaggtcacc tgcttgagat tgaaattgag aagcaatttt ctttaaatct tcattattaa
    26101 aaaatttgtc aatagaagat ttgaaggttc catctttttc atatttaata taaatcagtc
    26161 cttttgctcc aatttgaggg cgtttgacaa actctgtcaa ttcatcaagc tgctttctgc
    26221 tgtaatccgc acaacctgtg acacaaattc cgccaacata ttcaccatta tcaaaaatag
    26281 aaaattgatg tccttgaaca acgtctttca attcaacgaa tgtcattcca aaacgaatat
    26341 ccggcttatc agaaccatac agtttcatag catcagccca tctcattctt ggaaatggtt
    26401 gaataaaatc aacatttttg agggttttaa aaagatattt gattagtcct tcaaaagttt
    26461 gtaacacatc ttcttgcgtt acaaaagaca tttcgcaatc tatttgtgta aattcaggct
    26521 gacgatcggc tcgcaaatct tcgtcgcgaa agcattttac aatttgaaaa tatcgatcaa
    26581 aacctgctac catcaataat tgtttcaaaa gttgtggcga ttgcggtaac gcataaaatt
    26641 ctccaggatt cattcgtgat ggtaccacaa aatctcgtgc accttctggt gtagaagcaa
    26701 ttaaagcagg tgtttctact tcaatgaaac ccaactggtc gagataacgt cgagtttcaa
    26761 tagttaattg atgacgtaat tcaaggttct gtcgaataat gtttcgtcgt aaatccaaat
    26821 atctgtattt catcctcaag tcttcgccac catctgtatt gtcttctatg gtgaatggcg
    26881 gtgtttttga ttcattcaat atattcaatt cctcaacgag gatttctatt tcgccggttg
    26941 gaagattcaa atttttattg cttcgttctg ctatttctcc tgttgcttga atgacaaatt
    27001 cgcgccccaa ttcattagct tctttccaga gattttcgtt aatctgttga ttgaaaacta
    27061 attgtgttat tccgtatcga tctcgaaggt caacaaaaac cattcccccc atttttcgaa
    27121 tgcgttgtac ccagcctgcc aaagtcgttt tttgaccaac attggtgaga cgtaattcac
    27181 cacaagtatg tgttctgtac attttttata ataataatta atttttttaa aaattatctc
    27241 accatcacat ttggattaat ttttttaatc aaacctttta gtacgtcgcc aggacctaat
    27301 tctataaatt ctgtagcccc atcattaatc atgtttttaa tggtttgtgt ccatcgaaca
    27361 ggtgcagtta actgagcaat gagattttgc ttgatatttt caggattggt ttgaggatag
    27421 gcatttacgt tctgataaac tgggcagata ggtgtagaaa aagaagtttg atgaatagct
    27481 tggctaagtt caattctggc cgattccatc aaaggtgaat gaaaagctcc gccaacacta
    27541 agtttcagcg cgcgtttagc tcctttttct tttaataatt cacatgcttt ttctactccc
    27601 cttattgttc ctgaaataac gatttgtccg gggcaattat aatttgcagg tacaacaatt
    27661 tcatctttga tcgaatcgca aacttcttct acagtttcgt catctaaacc aatgatggcc
    27721 gccatagttg attcttccat ttcacaagct ttttgcatgg cttgtgcacg ttttgaaacc
    27781 aaaatgagtc catcttcaaa agtcaaagct tgagctgcca caagagctga aaattcacct
    27841 aatgaatgtc ctgctaccat atcaggtttg aaattatctt ctaaaacgaa agctgaaatt
    27901 actgaatgca aaaatatggc tggttgagtt actttggttt gtcgtaaatc ttcatcagtt
    27961 ccttcaaaca ttaaatcagt aatgcgaaat cccaaaattt cattggcttt ttcaaaatac
    28021 ttttttgcta tctcagattg ctcgtataaa tccttaccca tgcctacaaa ttgggctcct
    28081 tgaccaggaa aaacgtatgc tttcattttt gataaattta taaacagtta attatattaa
    28141 ttctttaaaa aattttatta aaagatctaa agacaaaata tcaagaaagg tttggcaaag
    28201 ttaataaaaa agatatatgt aaacttcaat gtgaagattt aactataaac aaattaaaaa
    28261 atagatttat aaattattgt ttttcaatta tttagaaatt atattaaatt ggatttttac
    28321 tttttaataa aaaacctata attttgaaag gtaaaacatt gaagatatgg atttaatttt
    28381 aaaatatttt cctcacctca ctcctactca aatttcccaa tttgaagcac tttacggact
    28441 ttattctgat tggaatcaaa aaattaatgt catttcacgg aaagatatag aaaatttata
    28501 tgaacatcac gtattacctt cattaggaat tgcacaagta attcattttc aaaaaggaac
    28561 ttccattctg gatgttggta caggtggcgg ttttccagga attccacttg ctattttgtt
    28621 ccctgaggta aattttacct tgattgattc agtcggaaaa aaaattaagg tagcttcgga
    28681 aatttcaaaa gcaattgccc ttgaaaatac agtttttaaa caaataagat tagaagaaga
    28741 aaaaggtcaa tatgatttta ttgtgagtcg tgctgtaatg acgatgaatg aattggtaaa
    28801 acaaggtagc aaaaacatct taaaacgcca aataaatgcc cttcctaacg gtattatttg
    28861 cttgaaaggt ggagaaattt ctaatgaaat tcgtaactat aaaaatattg ctgaaatttt
    28921 tccactttcc aattatttca aagaagagta ttttaagact aaaaaaataa tttatatgcc
    28981 tgtttttaag taaaatattt taacgagtgg aaaaatatga aaatcaaaaa atttgttttt
    29041 aatccttttg aagaaaatac ttatattgtt tcagatgaaa ccaaagaagc tatcgttatt
    29101 gatgctggct gtttggataa agaagagaaa agattattaa aagaatatat agctgacaat
    29161 cagctaatta taaaatatat tttaaatact catttgcata ttgatcatca gtttgggaat
    29221 cgttttttac ttgaaaatta ttttattgct cctttagctg gcaaagaaga cgaatttttt
    29281 ttagatactt ttagagaaca aacttatttt tttggaatta aagttcaaga ggagcccgct
    29341 ccacttggtg gatatttaat tgataatcaa aaaataaatt ttggaaatac ttctttacag
    29401 gtttttcatg tgccaggcca ttcaccagga agtatggctt tttattttga aaatgaaaag
    29461 gcaattttta cgggtgacgt actttttaaa ggaactattg gaagaactga ttttgaaaaa
    29521 ggagactttg atactattat tcgttctatt atacagcgtt tacttccact tcctgacgaa
    29581 accgttgttt attctggaca tggacctatt actaccattg gaaatgaaaa aaaatataat
    29641 ccttatctac aaaattattg ctgatataat ttattttcga taataaactg ataaacagca
    29701 ggatgtagcc atttgtcaac atctttttgc tcttttaaag cttgtcggat agtagaagaa
    29761 gaaatatcat aataaggtgt ggaaagaaat ttcatttgag ggtatttgga aagtaaatga
    29821 gaagcatcaa aatttttcct gggataaaca agtaccggat attcttccag aattttttga
    29881 taatctttcc attgatcgaa aattaaaaca ttgtcgctcc cgatcagcaa ataaaattta
    29941 ttttctggat atttttcaga aaaagcatgt aaactgttga tggtataaga cggtttaggt
    30001 aaagagaatt ccaaatcaga aacttttaat tggggatggt tttgaatagc taattcaacc
    30061 atcttcaacc gaatattttc atcaactaat tctgtgtcct cttttaaagg attgtgaggt
    30121 gaaacaacaa accaaacttc gtcaacatat tgattgtcaa ttatatattg agctaaattt
    30181 acatgaccca aatgaactgg attgaaagaa ccaaaatata gaccaatttt tttattcgta
    30241 ttttcagttt gagtattcat taatttcgaa aattatttat tcagaaaatc tcgaacggtt
    30301 tcttcaattt ccttttttgc tttttccaga tcatcattaa tgataatttt atcaaattgg
    30361 ggagcaaaac tcatttcata agcagctttc tctattcttt tctgaatcat ttcaggtgtt
    30421 tctgttcctc ttttgagcaa acgttctttt aatgcctcca tgttaggtgg agcaataaaa
    30481 atagccaaag cctgattacc aaattgtctt tttatgttga gcccaccttt tacatccaca
    30541 tcaaaaatga cattcttatc tttttgggtg attctatcaa tttcacttcg caatgttccg
    30601 taaaaacaat ctggataaac ttcctcgtat tctaaaaatt ctccattggc aattttttgt
    30661 ttaaattctt caggtgtcaa aaaataataa tcaatgcctt ctttttcatt cagacgtgga
    30721 gcacgactgg tagcagaaac agaaaattca agattcaaat tgctatttaa taaatgatgt
    30781 actaatgtac ttttccctgc acccgaaggg gctgataata taattaattt acctgccatt
    30841 cgcattgttt tttaaaatga aatccaaaaa attttgacca aaattccatt ttcttttctt
    30901 gaaccttaaa actcgtatca aacatctgta ccacaatttt agagaacatt caaaacctgc
    30961 tctttgattt gttccagttc atctttcatt ttgacaacaa ttttctggat ttcgctgtga
    31021 tttgcttttg cccccagcgt gtttatttcg cgtccgattt cctgagcaat aaatcctaat
    31081 tttttacctt gtgcctgttc ttcttccaat acctgaataa aatagtttaa atgattttgc
    31141 aaacgagact tttcttcatt gatatcgagt ttttcaatat aatatattaa ctcctgctcc
    31201 attctgtttt tgtcgtaatc aaccttatct gaaatacttt ccagttcttc tttcagtttt
    31261 tccttaattt tttcaattcg ttcagcttcg aaaggagcaa tttcgttgag caattgctga
    31321 atattgctta ttttgttata aaacacattt tcaacggctt ttccttcttg catacgaaaa
    31381 gtctttaact catcaaaagc ctgctgtatt gcttttttta ctgcttgcca ttcattttcg
    31441 tccaattctg aaatttctgt tttcaaacta tcaggtaaac gtagaaaaat atcaaaccaa
    31501 ttgttgggag gattaatacc taaatattct gataagtcgt tgatttgttg ataataacct
    31561 tctattatag tctggttaaa ttgtgttgcg gtttctttgc ctgcattctc aataaacaaa
    31621 gaaaaatcaa tttttcctct ttcgagtttt tgagaaattt catttctaat ttccaattct
    31681 ttttcccgat acaaatttgg aattttaata gaaacatcga gttgcttact attaagcgat
    31741 tttacttcta ttacaatttt tttgttatta tattcgcatg tagctttccc aaaacccgtc
    31801 atcgattgta acataatctt aattttttaa actattcgaa aaaaaattga agtattctat
    31861 gaacaagtaa attaatcaat ttgatttaat tattttaaaa tcagcttatt agttattaaa
    31921 ataattaata tttagaagtt cctctgcatt tttaagagaa acttccgtaa cattttctcc
    31981 actcaacatc tgagcaattt cttgaactct ttcttcttga tttagaggaa caatatgtgt
    32041 ctgggctttt tcgcctgatt catctttata tacgcgataa tgatactctc ccttcgaagc
    32101 aatttgagga agatgggtga tggcaattac ctgcatatct ttgctcatat cgtgcataat
    32161 ttgacccatt ttatgagcaa tttcacctga aacacctgta tcaatctcat caaaaataat
    32221 ggttggtaaa ccagctcgat gagccactaa cgatttgatt gacaacatca atcgtgaaat
    32281 ttcaccgcca gacgcaattt gagcaactgg ttgtaaagat tgatttttat ttgcagagaa
    32341 aagaaattgc acattatctt ttccattttc tgtaaaatca aaagtttctg aaatttcaac
    32401 tttaaattga atatatggca tacccagttg agaaagttgt tgctttaagt aattttcaat
    32461 agattccgta gcttttattc gagtttccct aagtatgttg gcttgttgaa tcaattgttc
    32521 ataaacctca ttaatttttt tctgtaaatc agccaattcg tcatcaaaag attcaatttt
    32581 ttccaattga tcttttatat tatctctgat agaaataagt tctttctcgg ttgatacctt
    32641 gaatttcttt tccaaagcat aaatttcgtt caacctgttt tcaatccatt gtaatctatc
    32701 tggttgaaat tcaattcttt cttccaatga aataatttcg ttggcaatat cttttgtttc
    32761 aatttctact gattgtaaac gttgatacca attttcacct tcaggaataa aattttttat
    32821 atgatccagc gaattaatag catttttaat catggaaatc gccatttgtt cctcattcag
    32881 aaaataataa gctttttgca attcttcctt aatttcttcc gtgtgactta atgtttgaaa
    32941 ttcagcttct aattcagttt gttcgttctc agaaagatga gcatcattaa gttgctgaaa
    33001 ttggaattgc aaataatcaa attctgccag ttgtttttca gcttcttttt gtaattgacg
    33061 gaaatcgttt tgtagtgttt tccattcgaa aaaagtaatt cggtaattct cccgaatttt
    33121 ttcgttttga gccattgtat ccactacttg caattgataa tcggcatgtg atagcagtaa
    33181 attttcgtat tgtgaatgga tgtcaatcag tttatcgcca agttcacgta atacttgcaa
    33241 agaaacaggg gtatcgttta caaaagctct tgattttcca gcgacagtga tttctcgtct
    33301 taaaaggcaa ttggaagaat tataatccag ttcatttttt tcaaaaaaat cttctaattg
    33361 ataatcgtga atatcaaatt ttgcttcaat cacacacttt tcttctcctt ccttaataag
    33421 cttactatca gccctttgtc ccaataagag agaaagtgct cccagaataa tggattttcc
    33481 agcacccgtt tcacctgtaa tgactgaaaa accactttca aaattgatat ttatttctga
    33541 aattaaagca aaattactga tataaagcga tttaagcatt tagatttcaa gttagacttt
    33601 aattttgtaa aaaattcaat tcacttttta cttgctattt aaaatttgct catatttagc
    33661 agtttgggca ggatcaacat ctgaaaggat ttctactacc agtatttttt ccttttcggt
    33721 tccttttgaa aatatattca taagttcatc tgttttagca tctaaaaaag aagaaataac
    33781 aacagcggat ggtcgagccc tattgacatc acgcaagacg tgcaatcctt cagctatttt
    33841 tgcacgtgcg ttagctgcat tgtccaccat catatccagc cctaaacgat gatactcata
    33901 aaaataattc ctaaaaggtt taaaagcttc atccaataaa ttactaacca aagcataacg
    33961 atttcgcgga ctttcaaaag ctttccatcc agataaatta attccttgtg ccatgtttac
    34021 aatgttctct gctgcctgaa aagcagaagt gcctcccaat cgagaaaaag aatccatatc
    34081 ataaccaatg atgatatagg cataatatga cagaacagca gttaaattgg aagtaataac
    34141 agagggattc cattccagtt gatcaaactc tttgtaggta aacgtaaaat cgttgtcctt
    34201 gaaattaaaa agtgtggtgg tatagcttga attataaacg ggacgacgtg attgtacaag
    34261 aatttctgct gtaaaattgt ctccttctac ttttttaacg gtaatgttca tagtacattc
    34321 tattcgttct tcgggagtga aagtcatttc tgtccatttg cgattgttta taaattcata
    34381 catggatttt tgcaatgttg taaaaaccga tttattggtg ccttgaatta ggtcagaatt
    34441 aatattgata ttacaattca attcttgagc agaaacaaat tgaaaaagag aaaaaaacaa
    34501 aattgtttct aaaaacttaa tttttttcat ttttcggtgt ttaacatttg aattaattca
    34561 ttgacaatat cctcagctac ttctttttta ggttttaaat tgaatgcttt cttttcgcca
    34621 gaaggataaa aaatcgtaat tttattagta tcataaccga aaccagcttt ttcatcattt
    34681 aatgaattaa ggacaataaa atcaaaatct ttctttttga gtttatcaaa tgcatttttt
    34741 tcttcattgt tggtctcgag agcaaatcca atcagaaatt gcttttcatt tttcattttt
    34801 ccaagacttg ctgcaatatc gggtgtgggc tttaattcca gcattaaatt atcgttttca
    34861 tgctttaatt tattttcgga tttttctaca ggagtaaaat ctgcaacagc cgcagaaaga
    34921 atggctccat ccatatcttt aaaatggttt gttacagcat gatacatttc ttcggccgaa
    34981 gttacattga tttgttgaac tttaggatga tgaatagaaa gatgaacagg acctgtaatt
    35041 aaaagaactt ccgcaccttg acgggcacaa acttctgcca gagcaaatcc cattttcccc
    35101 gaagaaaaat taccaataaa acgcactgga tcaatctttt cgtaagttgg accagccgta
    35161 attagaattt ttttaccagt taaattttta ggagtaaaat aatcttccag aaaatcaaca
    35221 attttttctg gttcctccat cctacccttt ccttctaatc cactcgccaa ttctccactg
    35281 gaaggttcga taatgaaatt tccttgttgc tgtaatttat aaaggttttc ttgtgttatt
    35341 ttattaaaaa acatgtttac atccattgca ggagcaataa atactggaca tcttattgat
    35401 aaataagttg ttagtaataa attatcagcg attcctgcag ccatttttgc aatggtattt
    35461 gcagtagcag gagcaattaa aaaagtgtca gcccaagttc ctaaatctac gtggctgttc
    35521 caattgccac tggtaggatc gaaaaaatca actaaaattg gatttttagc aagtgttgcc
    35581 agcgtaagcg gagaaataaa ttctttagct gtcggcgtca taacaacctt tacctgtgct
    35641 ccttgcctga ctaataaacg aataagctga gcagttttat aagcagcaat gcttcctgtt
    35701 actcccacta atattttttt atctttcaac atattagttt catttgtaat aatagaattc
    35761 tgcacaaa
//
LOCUS       DAOSAS010000003        34949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_899_length_34949_cov_13.571388, whole genome shotgun sequence.
ACCESSION   DAOSAS010000003 DAOSAS010000000
VERSION     DAOSAS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 34949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34949
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_899_length_34949_cov_13.571388"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   1..1332
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..29
                     /rpt_unit_seq="cttttaatcgcaccagaaggaattgaaat"
     gene            complement(1468..2622)
                     /locus_tag="PLR72_00280"
     CDS             complement(1468..2622)
                     /locus_tag="PLR72_00280"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOK35685.1"
                     /translation="MKIPKSFKLIILFVVLILMSRTANADSICSHNNFRFIEIIGQAG
                     GHIYSADALKEASLNKYKEYRTLQLRYGWNSSDPNGWQSMYLYPQYGIGWYSGSIGDP
                     EIIGRPSAVYGFISFPLSYDPKHYPFIEASMGVGYHFKPYNKEHNYQNDAIGSKITGY
                     LNLKLAANYRLNRELDLIYGFDIIHFSNGRISKPNAGLNMLGMNVGLRYNYNTMQKEV
                     DNSCHPKVVLDARPQHSIFRPKESIKRSEFLLYSAFGLVQNDKDNSKHYGTSSTCIEY
                     RYLINTCSGVTAGFDFFYDSSLKPFYPDESYDLYGVHIGYDFLFWKFGARLQGGTYLT
                     QKGRNFKGHFFFRPALQYNIGSKFYAQVGLKTQKFKADWVEWGIGVKFPC"
     gene            3000..3326
                     /locus_tag="PLR72_00285"
     CDS             3000..3326
                     /locus_tag="PLR72_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOK35686.1"
                     /translation="MDKLKISTAQFENHSGDKNYNLSVIENLTRKAAEDNSKVIAFHE
                     CCITGYTFARQLSKLQLVYLAEFVPEGESTERLIPIAAKYEMAILARLFFIILFHYSF
                     NRTRRN"
     gene            3556..4428
                     /locus_tag="PLR72_00290"
     CDS             3556..4428
                     /locus_tag="PLR72_00290"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOK35687.1"
                     /translation="MKVAIFGNIYRPMVLSCVKVLFDYFTQQKITMMCESQLYKFIEE
                     TKDFDLQGVEVIDTDFFEADLAVSIGGDGTFLNTAARIGNKGIPILGINTGRLGFLAD
                     ISSEEIIPAFEAILNSQYRIENRTTLLVKTSDETTFEYPYALNEVSVSKQDSSSMISI
                     NATVNGEFINTYQADGLIVATPTGSTAYSMSVGGPIVVPQAQDFILSPVASHSLNVRP
                     IIIPDSWIIELEVHSRTHSFLASLDGRSKVLNQTTKLIISKAPFTIKVIKQIHHTYFN
                     TLKNKLMWGMDKRN"
     gene            4788..5639
                     /gene="hisG"
                     /locus_tag="PLR72_00295"
     CDS             4788..5639
                     /gene="hisG"
                     /locus_tag="PLR72_00295"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOK35688.1"
                     /translation="MLRVAVQSKGRLFDDTISLLTEAGIKLEASKKLLLMPALNFPLE
                     VLFLRDDDIPESVAEGIADVGIVGENEFVEKKKKVKIIKRLGFSKCRLSLAIPKEIEY
                     TDISWFNGKTIATSYPTILNDFLKKNHIQANIHIISGSVEIAPNIGLADAIFDIVSTG
                     STLITNHLKEVEKVMESEALLIGNYQLTKEKQQILDEFIFRIEAVQMAEGKKYVLMNV
                     PTDNLNKIIEIIPGIKSPTIMPLAKEGWHSLHAVISEKEFWDIIEKLKACGAEGILVL
                     PIEKIIP"
     gene            5685..6413
                     /locus_tag="PLR72_00300"
     CDS             5685..6413
                     /locus_tag="PLR72_00300"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOK35689.1"
                     /translation="MNLCIDQGNSSTKAGIFDKDKLIQCFSYQNFNIKILLSIFSDFP
                     IENCIFSTVIEKDPILINFLREKIPFVIVLDDKTPIPIINKYLTPESLGNDRLAGVVG
                     ASFLKPNSDVLVVDAGTAITYDFIDANKTYCGGNISPGIDLRIKSLHEFTQKLPLVEP
                     TPNIPILGVDTYTAIAAGVIQGIAFEIDGYIDFLRLQYPKLLTFLTGGSVFYFDKKLK
                     NTIFVEKYLVLIGLNHILQYNVKP"
     gene            6400..7653
                     /locus_tag="PLR72_00305"
     CDS             6400..7653
                     /locus_tag="PLR72_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35690.1"
                     /translation="MLNHKVLFFIFATLMMMQLSYSQNNTNSPYTRFGYGEINDNHNG
                     EQRAMGGVAIGVRSSASINSVNPASYSVVDSMTFMFDMGASLLVSNFSSGNNKKTTFT
                     GNLDYLTIQFPLAKKIGVSAGIQPYSFSGYNFSETQLLFLANNTDTPDSLTATKSFVG
                     SGGFSEFYAGLSFELFHHLALGVNAYYMFGSINNYRNISFNQSTNFTSTTQLNYIEAN
                     NFRFRYGGQFFTTFAKKHEITLGLIYEQKKNFNGNASTIISGISVDTINYDLAFDLPT
                     IFGAGLYYTYHNKLSVGLDYSFQNWEEARFYGKTDTLANRHKIAIGGEYIPNPNGRKY
                     FEHIRYRVGFNTSNPYYKIEGISPGKNFGINFGVGLPLRYSRTIINASFEYGKIGNYS
                     KLKENYFKFTFNACINENWFFKRKL"
     gene            7680..9077
                     /locus_tag="PLR72_00310"
     CDS             7680..9077
                     /locus_tag="PLR72_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35691.1"
                     /translation="MKTKITILFLMGLILLSPKGFAQKDKKDKTKATDQEFTSTQNPS
                     QQVTDSMCLIYISLFNESAKNKQYADAYGPWLSAYNECPGANKVIYTRGREILQWKLG
                     QAKDQTSYKETFDELMRMYDKRIQYFGDDSRYPTPWILGVKALDYITYVKGDDLKKPA
                     YEWLEQSIDGMKENSELEVLRQFIMLSYNIYKAEPTHAEKFIADYLKVNEILEAQINN
                     PEFKYAELASQIKQGLDVMFAQSGAADCKTLDEIYKSKVSQNISNLEYLNNVLDFYSR
                     IGCFDSQVYFQAAIAAHKIQPTATSANACAEMSYKKKNFNQAIQFYEEATNLATNNNE
                     KADYLYKIAQIYYSELQNYLKARDYARKSLEYNPNNGSAYLLIGIMYANTKGIYDDPV
                     LAKTVFWVAVDKFIKAKQVDPDPKIREEADKLIQTYSRYFPSKEDIFFHPELEEGKSF
                     YVGGWIGETTICRPY"
     gene            9077..9715
                     /gene="lptC"
                     /locus_tag="PLR72_00315"
     CDS             9077..9715
                     /gene="lptC"
                     /locus_tag="PLR72_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOK35692.1"
                     /translation="MIQNLKNKFQLNYKNLTIIFNIVVMFFFMIGCNKEKPEIIQAIT
                     DRAATPNLHATDVTTIISDSGITRYRISAPQWDIYDKVPEPYWEFPYGIHLERFDMNL
                     HVDANIHSKYAKYYINQKLWELKGNVKATNLKGELFETEQLFWDQNSERIYSDSLIKV
                     TRPTQIINAIGFESDQSMNYYVFHKVKGIFPYEESQDSFPPNPQQTEISPSK"
     gene            complement(10063..11361)
                     /locus_tag="PLR72_00320"
     CDS             complement(10063..11361)
                     /locus_tag="PLR72_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOK35693.1"
                     /translation="MHFSFQHKFFLLAFLLSFSFALCVSSQSVKQLEEQRKQTLQQLE
                     ITNKLLNETKQSQKSSLNKLTILDKSIKERTRLVNTMNKEIEQLDIEMKKLAQEKAEL
                     EKKLQQLKNDYARLVQEAYINRSIYSKIMFVLSAETFDQSIRRLRYLKEYSDYRKMQV
                     KEIERVEKEIQQKNDSLAAHKQTLAQVVKQKEIETKKLSEEKKKENVVLNDLKKKEKN
                     LRSEITAQQKKANELNKRIEYLIAEEIRKAEAKKKAEEKLRQESAKGTSKKTAKEKSA
                     EQAKLAAAAALTKEEALLSDNFESNKGRLPWPVANGFISGHFGIQQHPVLKYVTTNNK
                     GIYIQTTANTDARAVFDGTVTQRFAVPGSNNAVIIQHGEYRTVYANLTEIFVTVGQKV
                     KAKQPIGRIYTDRDKDNKTELYFQLWKSKTLLDPEPWLAH"
     gene            complement(11388..12008)
                     /locus_tag="PLR72_00325"
     CDS             complement(11388..12008)
                     /locus_tag="PLR72_00325"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOK35694.1"
                     /translation="MNLKLDNREFNVTSSFSMIKDSAIHISIQPFLGLELYKIEMAPQ
                     IIKIFDKINQKYYVVNYEFLSDRLGVQIDFYHLQALLTNRYFCLAGSENGCRILTNNS
                     EKINFGFENENFKQSLTIIPNFYIHELLLLDKKSNSQLLATYDDFDTQDGVLYPRKVS
                     IQVNQPKKNVYCDITISKVSFNKEIKLVPTDEKRFKRAFIEQLLQK"
     gene            complement(12235..14010)
                     /locus_tag="PLR72_00330"
     CDS             complement(12235..14010)
                     /locus_tag="PLR72_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK35695.1"
                     /translation="MQHQFCYFVYLIVMLSFMSCSILKSNKNEQISERKSLNTIEQRQ
                     FDYFFYEAIRLKENGQLDEAIENFLMSFYLNPLDAEVQSQLGLLYNNAGLPNEAIQFM
                     ENAVNLQSNNWWYNMTLINMYSQQKKWENAIQLATVVQKKYPQKEDIYNVLSFLYKQT
                     GNIGKAINTYNQLENLVGVHEAISFEKIKLYFQLNKDKKALSEIDRLIDKFPSESRYQ
                     VLKGDLFLQTDQLEKAYQIYMKVMQDDPQNPYVYVSLSDYYKKINQPEKSLEAIVNAL
                     KSDQLDVDSKISILGQYMNQLIQDKEKLDEMDNLFKLLVERYPLEEQVHAYYALFLEY
                     QKRSDEAISELETMLNINPKNEQTWLQLIHIYWDQKNYEKIAEVAERAMKELPENPQF
                     YFYQGIAQFQLENYRLALKSLQLALTLIPDNNIQQKLKCDIYSQIGDTYYKLSKKDSA
                     FIAYEEALKADPENIYVMNNYAYYLALEKENLSKAERMSAKTVELQPRNSTFLDTYAW
                     VLFQEKNCSLAKFYIERAIDNLEKEQENGVIYEHYGDILWECDFDKEKAVEMWKKALE
                     EGNHSEELQQKIQKALSAMPENEQK"
     gene            complement(14059..14490)
                     /gene="dut"
                     /locus_tag="PLR72_00335"
     CDS             complement(14059..14490)
                     /gene="dut"
                     /locus_tag="PLR72_00335"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOK35696.1"
                     /translation="MKVKIVNRSKHSLPAYKTILSAGMDLQANLDEPVTLKPFERKLI
                     PTGLYVELPSGYEAQIRPRSGLAIQKGITVLNSPGTIDADYRGEICVILINLSTEDFV
                     VNNGDRICQMIIAPYTQVEWEETEELNHTLRGKGGFGHTGY"
     gene            14945..16540
                     /gene="pckA"
                     /locus_tag="PLR72_00340"
     CDS             14945..16540
                     /gene="pckA"
                     /locus_tag="PLR72_00340"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOK35697.1"
                     /translation="MTKIDVSKYGISNNIEIVYNPTYEQLFRAETDPSNNGFERGVVT
                     NLGAVAVDTGIFTGRSPKDKFFVKDEVTENTMWWDGEINKPITVEAWNDCKKLATDHL
                     SKAKKLYVVDAFCGTNKDTRMKVRFIMEVAWQAHFVTNMFIRPSHYELANFGEPDFTL
                     INASKVTDPNWKEHGLHSEVFVLFNLSQHMAVIGGTWYGGEMKKGIFSLMNFYLPQRG
                     IASMHCSANVGEEGDVAIFFGLSGTGKTTLSADPKRYLIGDDEHGWDDHGIFNYEGGC
                     YAKVIDLSKENEPDIWGAIRRDALLENVTVLPDGTPDFSKAASKTENTRVSYPIYYIN
                     KIVLPSKAGHASKIIYLSADAYGVLPPVSILDEKSAQYHFLCGYTSKLAGTERGINEP
                     VPSFSPAFGEAFLTLHPTIYAKVLAEKMKKHNAKAYLVNTGWNGTGKRISIKDTRAII
                     DAIIDGSIEKAPTTHIPIMNLTVPTSLNNVDPKILDPRNTYANSGEWEEKAKSLAKQY
                     IKYFEQYCDNVDARVLLASGPQL"
     gene            16657..17376
                     /locus_tag="PLR72_00345"
     CDS             16657..17376
                     /locus_tag="PLR72_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOK35698.1"
                     /translation="MKIDICFSPALYPYYQTSEARMVVMVDVFRASTTICAAFATGAN
                     AIIPVATIEEALRYKNQGWIVGAERNVEKCNFADAGNSPFEYTPEMVKGKEIVFTTTN
                     GTQAINSASSEWEVIIGCFSNLTAVTNFCLANKKEVLVLCAGWNNRFNLEDAVFGGAL
                     AERLIKEGNFEPCSDAVITAQSLWQLAGNDPENFLKNTEHAHRLFSHHLEADYYFCLQ
                     TDTTDVLPYYDKTSKKLLIKK"
     gene            complement(17623..18336)
                     /locus_tag="PLR72_00350"
     CDS             complement(17623..18336)
                     /locus_tag="PLR72_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_036612898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOK35699.1"
                     /translation="MSNPYFKFKKFTVFQDKCAMKVGVDGVLLGAWTPLENSMHILEV
                     GTGTGLISMMLVQRSNAFIDAIDIDKQAIFQAQQNINFSPWRNRIQLSEISFQEYVQK
                     TASKYDLIVSNPPYFVNSLKAPSVSRTQARHADTLSHSELIELGNQILKPTGNICLIL
                     PVKEGLKCYLHALELNMGCQQLVFVYPRIELPAKRLLLHFSKEDAVTRVSKLVIETTH
                     RHQYTPEFKALVSDFYLNL"
     gene            complement(18369..18839)
                     /locus_tag="PLR72_00355"
     CDS             complement(18369..18839)
                     /locus_tag="PLR72_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35700.1"
                     /translation="MKAKNYICIIILMLGFVNYLQAQLPETAVENSPEQEAAKQTARL
                     EQELNLTLEQTKKIYEINLRYAKQRQLCNKRSEVMELIKKKDVEIQQVLSGDQYQQLL
                     NKRNERSTMQVPTTEGIRNITPSYRPINERKSVESSPSNRMKQKNTSSKPSPRK"
     gene            19084..19578
                     /locus_tag="PLR72_00360"
     CDS             19084..19578
                     /locus_tag="PLR72_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOK35701.1"
                     /translation="MKKIGILSDTHGFLDDRVFEYFADCDEIWHAGDWGSMEVVNSLT
                     QFKPVRGVYGNMDGYDVRAVFPEYNRFLCEEVDVWLTHIGTQQGKYLPAIQSVFIHQP
                     PDLFVCGHSHILKVKYDKKFHFLYVNPGAEGKFGIHKIQTIIRLKIDRKEIKDLEVIE
                     IGEK"
     gene            19695..20273
                     /locus_tag="PLR72_00365"
     CDS             19695..20273
                     /locus_tag="PLR72_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOK35702.1"
                     /translation="MFQIDETLVSSDLITDRFACDLSFCKGICCVEGVSGAPLEKSEI
                     EIIEKILPIVWDDLSEKERQIIKNQGFWYIDEDGDTVTSLVKGKECVFTYFDADGTCK
                     CVFEKAFCEGKTDFPKPVSCHLYPVRLQNYDEFIAVNVHHWHHCLYGYKQGKNLQIPL
                     YRFLKDALIRRFGENWYGQLEKIAQEEESKKI"
     gene            20316..21560
                     /locus_tag="PLR72_00370"
     CDS             20316..21560
                     /locus_tag="PLR72_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOK35703.1"
                     /translation="MKTPKVEIITIGDEILIGQIVDTNSAWIAQELNKIGFEVFQITS
                     VHDEEKQIIDALHAAMKHVDIVLMTGGLGPTNDDITKKTLCKYFNTKLIFDSSILENI
                     KKVFASRPEFVINDLTRSQAFVPENCEVIQNTVGTAPIMWFEKDKKIIVSMPGVPYEM
                     KEAMSREVMPKLQAYFKTPKIIHKTVQVFGYSESDLAIKIADWEKNLPENIHLAYLPN
                     YGIVKLRLSGTSDNLLSLEFSLNQQIDALTLILGNAIVAYEDLPMEQVVAKLLLSQEK
                     TVVTAESCTGGNIAHRFTSHSGSSNYFKGSVVAYDNEVKINLLGVSTDDLEKFGAVSQ
                     PVVEQMAAGVRRLLNADIAVATSGIAGPTGGSPEKPVGTVWIAACSEEKLVSKLFHFS
                     NTREQNIERATQAALVMIKELL"
     gene            complement(21637..21712)
                     /locus_tag="PLR72_00375"
     tRNA            complement(21637..21712)
                     /locus_tag="PLR72_00375"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21674..21676),aa:Pro,seq:ggg)
     gene            complement(21839..25111)
                     /gene="carB"
                     /locus_tag="PLR72_00380"
     CDS             complement(21839..25111)
                     /gene="carB"
                     /locus_tag="PLR72_00380"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOK35704.1"
                     /translation="MEKELSLLQVKKHPYNGPEKGEIKKVLVLGSGALKIGEAGEFDY
                     SGSQALKALKEEGVQTVLINPNIATVQTSEGIADKVYFLPITPYFVERVIEKERPDGI
                     FLSFGGQTALNCGVALYQNNIFEKYKVRVLGTPVQAIIDTEDREIFNQRLSEINVKYI
                     QSQAVTHLNEAYEAAHKVGYPIIVRAAYALGGLGSGFCNNDEELKSVVEKAFSYSPQV
                     LIEKSLKGWKEIEYEVVRDRYDNCITVCSMENFDPLGIHTGESVVVAPSQTLTSSEYY
                     KLRELSIKIIRHIGIIGECNIQYALDPNSEDYRVIEVNARLSRSSALASKATGYPLAF
                     IATKLGLGYGLAELKNSITKVTSAFFEPAVDYVVCKIPRWDLNKFNGVSHLLGSSMKS
                     VGEVMSIGRSFEEIFQKGLRMVGLGMHGFVANRDYFVDDIDDALSQPTDKRVFYLAQA
                     LHEGYSIDRLYELTKIDRWFLYKLKHIIDLEKELMGYNSLSSLPDKLLYEAKKAGFSD
                     FQITRLVLKCSSDEMDDMIKVTREYRKSRNILPVVKQIDTLAAEYPAQTNYLYLTYNG
                     STHDIQYENDQLSVIVLGSGAYRIGSSVEFDWCSVNALMTIKKEGYRSVMINYNPETV
                     STDYDMCDRLYFDELTFERVMDIIELENPKGVIVSTGGQIPNNLALKLAKANVKILGT
                     QAEFIDMAEDRHKFSAMLDQLGIDQPRWKELTTFEDVKEFVNRIGYPVLVRPSYVLSG
                     AAMNVCYDDSQLENFLKVATEVSKKHPVVVSEFLERCKEIEIDAVANKGEIIAYAISE
                     HIEFAGVHSGDATTQFPPQKIYIETIRRIKNIARQIAHSLQISGPFNIQFLARDNYIK
                     VIECNLRSSRSFPFVSKVLKINFIDLATRIMLGLPVEKPEKSAFDLDYVGIKASQFSF
                     ARLQQADPILGVDMASTGEVGCLGDDFSEAVLKALLSVGFKIPEKNILISSGEPKSKV
                     DLLDACRLLYKKGYQIYATRGSHQFLAENGIESKVVLWPDEEGEYNVKDMIAQRKFDL
                     VINIPKNVTQRELANDYVIRRSAIDYNIPLLTNARLASAFIFAFCNIKLEDIKIKSWD
                     EY"
     gene            complement(25142..26209)
                     /gene="carA"
                     /locus_tag="PLR72_00385"
                     /pseudo
     CDS             complement(25142..26209)
                     /gene="carA"
                     /locus_tag="PLR72_00385"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
     gene            complement(27071..27826)
                     /locus_tag="PLR72_00390"
     CDS             complement(27071..27826)
                     /locus_tag="PLR72_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOK35705.1"
                     /translation="MEKTINITLKIWRQASAKAKGKFETYNLENVPTDSSFLEMLDIL
                     NEKLIKEHIEPIAFDHDCREGICGMCGLYINGHPHGPDTEVTTCQLHMRKFKDGDTIV
                     VEPWRSAAFPVVKDLIVDRSAFDKIIQAGGFISINTGGAPDANNILIPKDRADEAMDA
                     ATCIGCGACVAACKNASAMLFVAAKVSHLALLPQGKVEAAKRAKAMVAKMEELGFGNC
                     TNTRACEAECPKSVSISNIARLNREYLSAKFKD"
     gene            complement(27864..29840)
                     /locus_tag="PLR72_00395"
     CDS             complement(27864..29840)
                     /locus_tag="PLR72_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOK35706.1"
                     /translation="MKNNERTQEGENFILDTHIPEGPLAEKWTNYKAHQKLVNPANKR
                     KLDVIVVGTGLAGASAAASLGELGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDG
                     DSVFRLFYDTIKGGDFRSREANVYRLAEVSNNIIDQCVAQGVPFAREYGGLLENRSFG
                     GAQVSRTFYAKGQTGQQLLLGAYSALSRQIANGTVKIFPRYEMLDVVIIEGRARGIIA
                     RNLITGRIERYFAHAVVIASGGYGNTYFLSTNAMASNGSAVMQMYRKGAYFANPAFVQ
                     IHPTCIPLHGDYQSKLTLMSESLRNDGRIWVPKRKEDAEAIRAGKLKPTDLKEEDRDY
                     YLERRYPAFGNLVPRDIASRAAKERCDAGYGVGNTGLAVYLDFSDAIQRLGEEVIRER
                     YGNLFQMYEKIVDENPYKTPMMIYPAIHYTMGGIWVDYELQTSIKGLFCIGEANFSDH
                     GANRLGASALMQGLADGYFILPYTIQNYLADQIQVPRMDTDLPEFDEAEKNVTKRIKK
                     VINIKGQRTVDSIHRELGLIMWDLVGMSRNKEGLQIALEKIKNIRKEFWNNVYVPGDE
                     YDFNNELAKALRLADFIDIGELIARDALMREESCGGHFREEYQTPEGEALRNDEKFSF
                     VSCWKYTGEDTEPILLKESLKFEFIQLQQRNYKE"
     gene            complement(29885..30559)
                     /locus_tag="PLR72_00400"
     CDS             complement(29885..30559)
                     /locus_tag="PLR72_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOK35707.1"
                     /translation="MWLIDSPIGRKFIMSISGIFLVLFLLFHSCMNIVVIISPESYNQ
                     ICAFLGANWYAVIGSLVLALGFVVHILYALYLSFLNFRARGEVRYAVKATPTDVEWSS
                     KHMLILGVIILGFIVLHLYNFWFKMQFAELSGIHTGAFDPQNGAAYVSALFSHWIYCA
                     LYIIWLVALWLHLTHGVWSALHSLGLNNKKWLPRIKVIGNIVSTIIVLMFMSVPIYYL
                     IVNILQ"
     gene            complement(31355..32719)
                     /gene="miaB"
                     /locus_tag="PLR72_00405"
     CDS             complement(31355..32719)
                     /gene="miaB"
                     /locus_tag="PLR72_00405"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOK35708.1"
                     /translation="MKMNGILALELSKESQNKKLYIETYGCQMNVADSEVVAAIMKMD
                     GYELTTQLNEADAIFVNTCSIRDNAERRVIERLKYFQSFKRRNRKLIIGVLGCMAERI
                     KEDLITNYGVDLVVGPDAYLDLPNLISAVENGEKAVNVLLSTTETYREIIPLRMGNSV
                     SGFISISRGCNNFCSYCIVPYTRGRERSRDVESILHELDDLRARNFKEVTLLGQNVNS
                     YQFEYQEKMIEFPDLLKIVAERAPEMRFRFTTSHPKDMSDRTLEVIAQYPNLCHYIHL
                     PVQSGSNKILKLMNRKYTRESYLEKIDSIRRILPDAAIGTDVFCGFPNETEEDFQQTL
                     SLMEEVKFDMAFTFKYSERPGTYAAKNLPDNISEEVKLQRLNEIIGLQKDLSLQSNRK
                     DVGKIFEVLVEGFSKRSREKFFGRTSQNKVVIFPRKERKIGETVEVKITDFSSATLFG
                     EVVG"
     gene            33618..34907
                     /locus_tag="PLR72_00410"
     CDS             33618..34907
                     /locus_tag="PLR72_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK35709.1"
                     /translation="MPTAIEFNNISKQYRLGLVSTRTLSHDLNRWWKTQVLRKEDPYL
                     KIGEVNDRAQKRESDYVWALKDINLKVEQGEVLGIIGKNGAGKSTLLKILSKVTAPTT
                     GTIKVNGRIASLLEVGTGFHPEMTGRENIYMNGAILGMTKKEIDRKLDEIVDFSGVER
                     YIDTPVKRYSSGMTVRLGFAIAAHLEPEILVVDEVLAVGDAEFQKKAIGKMQDVSRGD
                     GRTVLFVSHNMESIQNLCNNGILLGNGSIIKSGKIEDVVNYYISGNLAFAEDVNSINN
                     IDKYRPKGTTKEVEFLYLKRVSNYRIDVNDPLIFKMGLKKNAKVKKIQLMALIDDIAN
                     GGNRVGMAFSEEILLPEHKNEFEVEFTIQHHNLTKSKYIMDFWIGIGDVTSTIKYYDM
                     LYDTLTFEINTCNGKYINEWHNYWGQNCYTSVNVVLK"
BASE COUNT        11773 a         6013 c         5596 g        11567 t
ORIGIN      
        1 cttttaatcg caccagaagg aattgaaatg ctcgtacttc aaaggagtaa atatctccgt
       61 aataagcttt taatcgcacc agaaggaatt gaaatgatga tatagaagca aaagaaaatc
      121 ttgcagccac ttttaatcgc accagaagga attgaaatgt ttaaaatcct gaaaatgatg
      181 aaagataata tgggctttta atcgcaccag aaggaattga aattgcttat gttgtaatgg
      241 cataagcaag cctttttgaa acttttaatc gcaccagaag gaattgaaat ttgatgaaaa
      301 atggcgcgat catctatatg ctatggactt ttaatcgcac cagaaggaat tgaaattcgt
      361 gtttgtcgtc agaaatttct tgaaaaaaag gcttttaatc gcaccagaag gaattgaaat
      421 cttttttttg catatattca ttgccagttg ttggggcttt taatcgcacc agaaggaatt
      481 gaaataatga taaaaatttc ttaaaaaaat gggatgaagg cttttaatcg caccagaagg
      541 aattgaaatc ttttttttgc atatattcat tgccagttgt tggggctttt aatcgcacca
      601 gaaggaattg aaatgatttt aacggaaatg aaataagata taaatacaat cttttaatcg
      661 caccagaagg aattgaaata aaagtcagtg acaaaattac tgaaattgaa agtcgctttt
      721 aatcgcacca gaaggaattg aaattaatgt tgatttgtct tccttctcgt ctttgtgttt
      781 cttttagtcg caccagaagg aattgaaatg aaacacaaca tttgtggatt gggatgggaa
      841 tatactttta atcgcaccag aaggaattga aatgcagtaa ggggaattgt tatatttttg
      901 ataacaacct tttaatcgca ccagaaggaa ttgaaattgt taaaattaag gtaataaaaa
      961 taagataagt ggccttttaa tcgcaccaga aggaattgaa atataaatat agactaacaa
     1021 aatacagaaa tgcaacttct tttaatcgca ccagaaggaa ttgaaattat tttgttgatg
     1081 aaaataaact tgttagttat atgtctttta atcgcaccaa aaggaattga aattagcatc
     1141 cacacgtcta gcactatcgg tgctgcacat cttttaatcg caccagaagg aattgaaatt
     1201 cgacagttcc tattttaaaa gaaaatttgc aggaaggctt ttaatcgcac cagaaggaat
     1261 tgaaattatg ttttggtaga agatggcaaa acgtgggctg gtgcttttca tcgcaccata
     1321 aagaatcgaa agttactgaa aattcgggtt ttcattaata tgctggagaa agtatgataa
     1381 caatccctgg ttggcgaaac agttttccgt cgaaaataac ccaaaatctg gatcccgata
     1441 aaaaacggcg atggtgataa tcttttgtca gcacgggaat tttactccaa tcccccactc
     1501 aacccaatcg gctttaaact tctgagtttt cagaccaact tgagcataaa acttactccc
     1561 aatgttgtac tgcagggcag gccgaaagaa aaaatgccct ttgaaattac gtcctttttg
     1621 agttaaataa gtccctcctt gcagccttgc accaaatttc caaaataaaa aatcgtagcc
     1681 aatatgtaca ccatacaagt cgtaactctc gtccggatag aaaggtttca ggctcgagtc
     1741 ataaaagaaa tcgaaaccgg cagttacgcc actacacgta ttgataaggt atctgtattc
     1801 aatgcaagtt gaggatgtgc cgtagtgttt gctattgtcc ttatcgtttt gaactaaacc
     1861 aaaagcgctg tatagaagaa attcgctgcg tttgatcgat tcttttggtc tgaatatact
     1921 atgctggggc cttgcatcga gaaccacctt ggggtgacat gagttgtcaa cctctttttg
     1981 catcgtatta taattgtatc ttagacctac attcatgccg agcatattta atccggcatt
     2041 gggcttagag attctgccat tgctgaaatg aataatatca aaaccataga ttaagtccaa
     2101 ctcgcggttt aatctgtaat ttgcagctaa ttttaaatta agataaccgg tgatctttga
     2161 accgatagca tcattttgat agttatgctc tttgttatac ggcttaaaat ggtagccaac
     2221 acccattgac gcctcaatga aagggtaatg tttgggatca taagaaagag gaaaagaaat
     2281 aaatccatat actgcacttg gccttcctat tatctctgga tcgccaatag acccactata
     2341 ccacccaata ccgtattggg gataaagata catgctttgc caaccgttag ggtcgctact
     2401 gttccatcca tacctcaatt gcaatgtcct atactcctta tacttattta gtgatgcttc
     2461 ctttagtgca tcggccgagt aaatatgccc gcctgcctgc ccaataatct caataaacct
     2521 aaaattatta tgagaacaaa ttgaatctgc atttgctgtt ctgctcatta atattaatac
     2581 tacaaaaaga ataataagtt taaaagattt cggaattttc atatatcttg agttttactg
     2641 actcaacagc cattaataat ttggtttaca cattgtagta aagcagtaaa ctggaaataa
     2701 aagtgtaacg actgcgggaa aaaatcaact ataacaaatt agggagcatg agtaaagtgg
     2761 ttatatatgt caaaaataca tatttttttc tgtaaaaacc ttgccttttt agttttttaa
     2821 aaataatttg cttattttct attcgatagc ttgttcggtg gaaccgcata agttgactta
     2881 agattgacac gcataaaatt atcgtgcata tttatttatc atggatattt gttgctgtta
     2941 caaaaaaaca aaaacgagaa aaattgccaa aactttctga ataagttaaa ttaaaatcta
     3001 tggataaact aaaaatttca accgcacaat ttgaaaacca tagcggagat aaaaattata
     3061 atttatcggt aattgaaaac cttactcgaa aagcagcaga agacaactct aaagtaattg
     3121 catttcacga gtgttgtatc acgggttata cttttgcccg acagctttca aaactgcaat
     3181 tggtgtatct ggctgaattt gttcctgagg gagagagcac cgaacgattg ataccaattg
     3241 ccgctaaata cgagatggct attttagcca gattgttttt cattattctt tttcattatt
     3301 cttttaatcg caccagaagg aattgaaacg acacagaatt aaataagaaa tggtgatggc
     3361 gcagttataa caggttatat tttagaggat attattcata taacttaatg atatttaaac
     3421 gcaatctgac aacaaatttt aatccctcaa atgcaaaaac gtttctatat ttctttatta
     3481 ttgtacaatt tgttttaaat ttgtgtgtat tttgtttttc gattatacaa ctaaatcaat
     3541 cataaacaac aatttatgaa agtagccatt tttgggaata tttatcgacc tatggtttta
     3601 tcctgtgtta aagttttatt tgattatttt actcagcaaa aaattaccat gatgtgtgaa
     3661 agtcaactct ataaatttat agaagagaca aaagattttg acctacaagg agttgaggtg
     3721 attgatactg atttttttga agcagatttg gcagtgagta ttggaggaga tgggactttt
     3781 ttaaatactg ctgcacggat tggaaataaa ggaattccaa tattaggtat caacacaggc
     3841 agattgggct ttttggccga catttcaagt gaagaaatta ttcctgcttt tgaagcaatt
     3901 ttaaattcac aatatcgcat tgaaaatcgg actactcttt tagtaaaaac ttccgatgaa
     3961 acaacatttg aataccctta tgccttgaat gaagtttcgg tgtcaaaaca agattcatct
     4021 tcaatgatta gtatcaatgc tacggtaaat ggcgaattta tcaacactta tcaagcagat
     4081 ggcttaatcg ttgccactcc aacaggttcc actgcttatt caatgagtgt agggggacca
     4141 attgtcgtac cacaggcaca agattttatt ttatcaccag ttgcttccca cagtttaaat
     4201 gtacgcccta tcattattcc tgatagctgg attatagaat tagaggttca tagccgtacc
     4261 catagttttt tggcgtcatt ggatggccga tcgaaagtgt tgaatcaaac caccaaactc
     4321 attatttcaa aagctccttt tactataaaa gttattaaac aaattcatca tacctatttt
     4381 aatactttaa aaaataaatt aatgtggggt atggataaaa gaaattaaag gataaaataa
     4441 attcaaaatc catcagtaaa aacttgattt taagtatttt aatttttttc tgttgttaat
     4501 atccacatta tcaatccctt agttaatcaa tttataaaac tatgccttat tcataagttg
     4561 gtttatgaat ttattctgct aaatgaatga tttttatcat agaatgtgta actttgaacc
     4621 tctgttgttg aatttttcca ctaaaaaacg tgtgaatttt tatcaagaaa gtcacaaagc
     4681 tcctttttat aaggcttttc gactttttaa tatcttttga agtggattta agactttact
     4741 aaaatcaaat agtaggatat ctataaaatc atgtaaatca aaaaaatatg ttacgagttg
     4801 cagtacaatc taaaggaaga ttatttgatg atactatatc gttactcaca gaagcaggga
     4861 taaagttaga agccagcaaa aagttattat tgatgccagc tctaaatttt ccactcgaag
     4921 ttttatttct gagagatgat gacattcctg aatcagtagc agaaggtatt gctgatgtgg
     4981 gtattgtggg agaaaatgaa ttcgttgaaa aaaagaaaaa ggtaaaaatt atcaagcgat
     5041 taggttttag taaatgtagg ctttcgttag cgatcccaaa agaaattgaa tacactgata
     5101 tttcctggtt taatggaaaa acaattgcta cttcttatcc taccatttta aatgattttt
     5161 taaagaaaaa ccatattcaa gctaatattc atatcatttc tggctcggta gaaattgccc
     5221 ctaacattgg attagctgat gctatttttg atattgtaag tacaggaagt acgctgataa
     5281 caaatcattt aaaagaagta gaaaaagtaa tggaatccga agccttactc attggtaatt
     5341 atcaattgac aaaagaaaag caacaaattc tggatgaatt tattttcaga attgaagccg
     5401 tacaaatggc agaagggaaa aaatatgttt tgatgaatgt tccaacagat aatttaaata
     5461 aaatcattga aattattcct ggcattaaaa gtcccaccat catgccatta gcaaaagaag
     5521 gatggcattc tttgcatgcc gtaatatctg aaaaagaatt ctgggatatt attgaaaaat
     5581 tgaaagcttg tggtgctgaa ggcattttag tgcttccaat tgaaaaaata atcccttaaa
     5641 ttcaatattc atggctgata taatttaatc tatttcaatt ttttatgaat ttatgcattg
     5701 atcaaggaaa ttcaagtacc aaagcaggaa tatttgataa ggataaacta atccaatgct
     5761 tttcgtatca aaatttcaat ataaagattc ttttatcaat tttttcagat tttcctatag
     5821 aaaattgtat attttcaact gttattgaaa aagatcctat acttatcaat tttttaagag
     5881 agaaaatacc ttttgtaatt gttttagatg ataaaactcc tatccctatt atcaataaat
     5941 atttaacccc cgaatcctta ggaaatgatc gtttagctgg agtggtaggt gcttcatttt
     6001 taaagccaaa ctcagatgta ctggttgttg atgcaggaac agcaataact tacgatttta
     6061 ttgatgccaa taaaacttat tgcgggggta atatctctcc aggcattgat ttaagaatta
     6121 aatctctcca tgaattcact caaaaattac cacttgttga accaacaccc aatattccaa
     6181 ttcttggagt tgatacgtat actgcaattg cagcgggtgt aatacaaggt attgctttcg
     6241 agatagatgg ctatatagat tttttacggt tacaataccc aaagctttta acttttttaa
     6301 caggaggtag tgttttttac tttgataaaa agctaaaaaa caccatcttt gtagaaaaat
     6361 atttagtact aataggcctt aaccacatac ttcaatataa tgttaaacca taaggtatta
     6421 ttttttatct tcgctacttt aatgatgatg caactatctt atagtcaaaa caatacaaat
     6481 tctccttata ctcgttttgg atatggagaa atcaatgata accacaacgg tgaacaacgt
     6541 gctatgggag gagttgcgat aggagtcagg tcatcggcaa gtattaattc tgtaaaccct
     6601 gcttcttata gcgtagtgga tagtatgaca tttatgtttg acatgggagc ttctttgttg
     6661 gtttcaaatt tttcaagtgg taataacaaa aagactactt ttacaggaaa tttggattat
     6721 cttactattc agtttccttt ggcaaaaaaa atcggcgtca gtgctggaat tcaaccatac
     6781 tctttttctg gatataattt cagcgaaact caactcctct ttttagccaa taatactgat
     6841 acacctgata gcctcactgc tactaaaagc tttgttggtt cagggggatt cagtgaattt
     6901 tatgctggtt tatccttcga attatttcac catttagcat taggcgtaaa tgcctattac
     6961 atgtttggaa gtatcaacaa ttatagaaat atcagtttta atcaatcaac aaattttact
     7021 tccactacgc aactaaacta tattgaagcg aataatttca gattcagata tgggggtcag
     7081 tttttcacaa cttttgccaa aaaacatgaa attacattag ggttaattta tgaacaaaag
     7141 aaaaatttca atggaaatgc ttcaaccatt atttctggca tttctgtgga caccataaac
     7201 tatgatctag cttttgattt gccaacgatt ttcggagctg gtttatatta tacttaccat
     7261 aataaacttt cagtaggact tgattatagt tttcaaaatt gggaagaagc cagattttat
     7321 gggaaaacag ataccttagc taaccgccat aaaatagcta taggaggaga atatattcca
     7381 aaccctaatg gaagaaaata ttttgaacat attcgttacc gtgttggctt taacacaagt
     7441 aatccctatt ataaaataga aggaatttct ccaggaaaaa attttggtat aaattttgga
     7501 gttggtttac cattacgata cagtagaaca ataatcaatg cttctttcga atacggtaaa
     7561 attggaaatt acagcaaact aaaagaaaat tattttaagt ttactttcaa tgcctgtatc
     7621 aatgaaaatt ggttttttaa acgaaaatta taaaacaact tttaaaaata ttttttatta
     7681 tgaaaactaa aataacaatt ttgttcttga tgggtttgat ccttctcagt cctaaaggat
     7741 ttgctcaaaa agataagaaa gataaaacaa aagctacgga tcaggaattc acaagtactc
     7801 aaaacccttc acagcaggta actgattcga tgtgtttgat ttatatcagt ttattcaatg
     7861 aatcagctaa aaataaacaa tatgctgatg cttatggtcc ctggttatca gcttacaatg
     7921 aatgtccagg agcaaataaa gtaatataca ccagaggtag agaaattctc cagtggaagt
     7981 taggacaggc aaaagatcaa acctcctaca aagaaacctt tgatgaatta atgagaatgt
     8041 atgacaaacg cattcaatat ttcggagatg acagtcgcta tccaactcct tggattttag
     8101 gagtaaaagc cttagactac atcacttatg taaaaggaga tgatttaaaa aaacctgctt
     8161 atgaatggtt agagcaatcc attgatggga tgaaagaaaa ttctgaatta gaagttctta
     8221 gacaatttat aatgttgtct tacaatattt acaaagcaga acctacacat gcagaaaaat
     8281 ttattgctga ttatttaaaa gttaatgaaa ttcttgaagc tcaaattaat aatcccgaat
     8341 ttaaatatgc tgaattagcc agtcaaataa agcaaggatt agatgtaatg tttgcccaaa
     8401 gtggtgctgc tgattgtaaa actttagatg aaatttataa atcgaaagta tctcaaaaca
     8461 tttcaaattt agagtacttg aataatgtgt tagattttta tagtcgaatt ggttgttttg
     8521 actctcaggt ttatttccaa gcagccattg ctgcccataa aatacaacca actgctacat
     8581 cggctaatgc atgtgctgaa atgtcataca aaaaaaagaa tttcaatcaa gcaattcaat
     8641 tttatgaaga agctactaat ttagcaacaa ataacaatga aaaggctgat tatttatata
     8701 agatagctca aatttattat tcagaattgc aaaattatct gaaagcaaga gattatgctc
     8761 gtaaatcatt agaatacaat cccaacaatg gcagtgctta tctgttgatt ggaataatgt
     8821 atgcaaatac aaaaggaatt tatgatgatc cggttttagc taaaactgtt ttttgggtag
     8881 cagtagataa gtttataaaa gcaaaacaag tagacccaga tcctaaaatt agagaagaag
     8941 cggataaatt aatccaaact tatagtaggt atttcccctc taaagaggat attttcttcc
     9001 atcctgaatt agaagaaggg aaatcatttt atgttggagg atggatagga gaaactacca
     9061 tttgcagacc atattgatga tacaaaattt gaagaataaa tttcaattga attacaagaa
     9121 cttaacaatt atttttaata tagttgttat gttctttttc atgataggtt gtaataaaga
     9181 aaagcctgaa ataattcaag ctattacgga cagagcagct actccaaacc ttcatgcaac
     9241 cgatgttact actattattt ccgattccgg aattacccgc taccgtattt ctgctccgca
     9301 gtgggatatt tacgataaag ttccggagcc ctattgggaa tttccttatg gtattcactt
     9361 ggaacgtttc gatatgaatc tgcatgtaga tgccaatatt cacagcaaat atgcaaaata
     9421 ttatattaat cagaaactgt gggaattaaa aggtaatgta aaagcaacca acttaaaagg
     9481 agaattattt gaaactgaac aacttttctg ggatcaaaac agcgaacgaa tttattctga
     9541 tagtttgatt aaagtaacac gtccaactca aattatcaat gccattggat ttgaatcgga
     9601 tcagtcaatg aattattatg tcttccacaa ggtaaaaggt atattcccat acgaagaatc
     9661 acaagattct tttcctccca atccgcagca aacagaaatt tcaccttcaa aataagaatt
     9721 ttccaaaaaa gctaccagca aactaattac tacaaaacaa aaatccatta aatccagatt
     9781 cagcattaga aagaaatgca gttacagtta attgtaaatc agacgataag gtgcaaaatt
     9841 ttctaatgcg tattgttaga aatattttag aaaaaattag ggcttaatta tttataatga
     9901 gcaggatata aagcaattcg atctgaaaaa atgaaatttt ctatttttga aagcttaatt
     9961 tggggtaaaa aacaatcaaa tttaaaaata taatgtctga acatcaattt attgaaaaca
    10021 aataatctta aaaacatttc tttaatcgga cagtaatgct ttttaatgag ccagccacgg
    10081 ttcaggatca agtaacgttt tacttttcca caactgaaaa taaagttcag tcttattatc
    10141 tttatctcgg tcggtataaa ttctaccaat aggttgtttt gcttttacct tctgccctac
    10201 tgtaacaaat atttcagtca aatttgcata cacagtacgg tattctccat gttgaattat
    10261 tacagcattg ttgctgcccg gtacagcaaa gcgttgcgtg acagtgccat caaaaactgc
    10321 tcttgcatcg gtattggcag tagtttgtat gtaaattcct ttattattgg tagtaacata
    10381 ttttaaaaca ggatgttgtt gaataccaaa atgtccacta ataaaaccat ttgctactgg
    10441 ccagggtaat cttcccttgt tgctttcaaa attatctgaa agcagtgctt cttctttagt
    10501 caatgcagcc gctgccgcta actttgcttg ttctgctgat ttttctttag cggttttttt
    10561 agaagttcct ttagctgatt cttgccttaa tttttcttct gctttctttt ttgcttcggc
    10621 tttgcgaatt tcttcggcaa ttaaatattc gatcctcttg ttcagctcat tggctttctt
    10681 ttgctgtgca gttatttcag atcgtaaatt cttttctttc ttttttaggt cattcaacac
    10741 gacattttct ttctttttct cttcggaaag ttttttggtt tcaatttctt tttgttttac
    10801 aacttgagcc aacgtttgtt tatgagcagc cagactatca tttttctgtt gaatttcttt
    10861 ttcaactctt tctatttctt tcacctgcat ttttcggtaa tcactgtatt cttttaaata
    10921 tctcaaacgt cgaatagact gatcaaatgt ttcagcagaa agcacaaaca taatttttga
    10981 ataaatactt cgattgatat atgcttcctg aaccagcctt gcataatcat tttttaattg
    11041 ctgaagtttt ttttcaagtt ccgccttttc ctgtgccaat tttttcatct caatatctag
    11101 ttgttctatt tctttgttca ttgtgttgac cagtcgggta cgttctttga tgctcttatc
    11161 gagaatggta agcttgttca atgaactttt ttgagattgt ttagtttcat tcaacagttt
    11221 attagttatc tcaagttgtt gcaaagtttg ctttcgctgt tcttcaagtt gttttacact
    11281 ttgtgatgaa acacaaagtg caaaagaaaa actcaataaa aaagcaagta aaaaaaactt
    11341 atgttgaaat gaaaaatgca taggattttt aaaataacag agaacactta tttctgtaat
    11401 aattgttcaa tgaatgctcg tttaaatctt ttttcgtctg ttggaactaa ttttatttcc
    11461 ttattaaaag agactttaga aatggttatg tcacaataaa catttttttt aggttgattc
    11521 acctgaatag aaacttttcg tggataaagt acgccatctt gtgtgtcaaa atcatcgtaa
    11581 gttgccaaca attgagaatt acttttctta tccaacaata gaagttcgtg aatatagaaa
    11641 ttaggtataa tggttaaaga ttgtttaaaa ttttcatttt caaatccgaa attaattttt
    11701 tctgagttat ttgtcaaaat ccgacaacca ttttcactac cagccaaaca aaaataacgg
    11761 tttgttaata aagcctgcaa atgataaaaa tcaatctgaa ctcctaaacg atcactcaaa
    11821 aattcataat tgacaacata atatttttga tttatcttat cgaaaatttt aattatctgc
    11881 ggagccattt caattttgta caactccaat cccaaaaaag gctgaataga aatatgaata
    11941 gcagaatctt taatcattga gaaagaagaa gtaacattaa attctctatt gtctaatttt
    12001 aaattcattt tgattttact ggcattcata ctttgaatat tgtcctgcaa ctttaagtat
    12061 tcaattacct tttgagtgtt taaatctttt ggcaactgtg taaccaagac ctgtttagtt
    12121 gttttacagg agctcaatag caacaaaaag aaaaagaata ttttaatttt ttgtttcatg
    12181 aaaggcaaag ttttaaaaat aacaaagtaa aattatttga aagcaacgat tttctcattt
    12241 ttgttcgttt tcaggcatag ctgaaagtgc tttttgaatt ttttgttgaa gttcctccga
    12301 atgattccct tcttctaaag ctttcttcca catttccaca gctttttcct tatcaaaatc
    12361 acactcccac aatatatctc cgtaatgttc ataaattaca ccgttttctt gctctttttc
    12421 gagattatca atggcacgtt caatataaaa ttttgccaat gaacaatttt tttcctgaaa
    12481 taatacccac gcataagtat caagaaaagt actatttcga ggctgtaatt ctactgtttt
    12541 agcactcatt cgttccgcct tacttaaatt ttctttctcc aatgctaaat aatacgcata
    12601 gttattcatt acataaatat tttcaggatc agctttcaaa gcttcttcat aagctataaa
    12661 agcagaatct ttcttgctga gcttataata agtatcacct atttgagaat aaatatcaca
    12721 tttcagcttt tgttgaatgt tgttatcggg aataagagtg agagcaagct gcaaactttt
    12781 taatgccaac cgataatttt ccaattgaaa ttgagcaatg ccttgataaa aataaaattg
    12841 aggattttca ggaagttctt tcattgctct ttcagcaact tctgctattt tctcataatt
    12901 tttttgatcc caataaatat ggattaattg cagccatgtc tgttcattct ttgggttgat
    12961 gttcaacatc gtttcaagtt ccgaaatcgc ttcatcactg cgtttttgat attctaaaaa
    13021 caaggcataa tatgcgtgaa cttgttcttc gagagggtat ctttctacta acaacttaaa
    13081 aagattgtcc atctcatcca atttttcctt gtcctgaatc aattgattca tatattgacc
    13141 taaaattgaa atttttgaat caacgtccaa ctgatcactt ttgagggcat tcacaattgc
    13201 ttcgagcgat ttttctggct gatttatttt tttataatag tctgataaag aaacatatac
    13261 ataaggattt tgaggatcat cttgcataac tttcatataa atttgataag ctttttcaag
    13321 ttgatctgtt tgaagaaaaa gatccccttt caaaacttga tagcgacttt cagaaggaaa
    13381 tttgtctatt aacctatcaa tttctgataa tgcctttttg tccttgttca attgaaaata
    13441 aagttttatt ttttcaaacg agattgcttc atgaactccc acgagatttt caagttgatt
    13501 ataagtatta atggctttac caatattacc tgtttgttta taaagaaaag ataaaacatt
    13561 ataaatatct tccttttgag gatatttttt ttggaccaca gtagctaatt gaatagcatt
    13621 ctcccacttt ttttgttgtg aatacatatt aatgagagtc atattgtacc accagttatt
    13681 cgactgcaaa ttcactgcat tttccataaa ttgtatggct tcatttggca agccagcgtt
    13741 attataaagc aaaccgagtt gtgattgtac ctctgcatcc agcggattaa ggtaaaaaga
    13801 cattaaaaaa ttttcgatgg cttcatctaa ttgcccattt tctttcaagc gaattgcctc
    13861 ataaaaaaaa taatcaaatt gccgttgttc aattgtatta agtgacttcc tttcagaaat
    13921 ttgctcattt ttatttgatt tcagaatcga acaagacata aaactcagca tcacaataag
    13981 gtaaacaaaa taacaaaatt gatgttgcat gaaaattgtt tttaaaatta taagaacaac
    14041 gacataaaag attaattatc aatagcctgt atgaccaaaa ccgcctttac ctcgtaaagt
    14101 gtgattaagt tcttctgttt cttcccattc tacttgagtg tacggtgcaa taatcatttg
    14161 acaaattcga tctccattat taacaacgaa atcttctgtt gacaaattga taagaatcac
    14221 acaaatttca cctcgataat cagcatcaat cgtgccagga gaattgagca cggtaattcc
    14281 tttttgaatt gccagaccac ttcttggtct tatctgagct tcgtaacctg atggtaattc
    14341 aacatataga cccgtaggaa ttaattttcg ttcgaaaggt tttaaagtga ctggttcatc
    14401 aagatttgcc tgtaagtcca ttcctgctga taatatagtt ttatatgccg gcaatgaatg
    14461 ttttgagcga ttaacaattt ttactttcat aaagttaatt tgtaatgctt ttataaaatt
    14521 tcaaaaatat atttttacct ccatcacatg agggattatt acattttctt tttaccttaa
    14581 cttctctaca aaaatacaat ttttattcta aaacgacttg tcttccttaa taaatatatg
    14641 cttttttcaa tttttcagga ttttttaaat ctaaattcat aaaattttaa tggacaataa
    14701 aaaataattt tttgacttta tatttttgac aaaatgttta atgaattgtt atataagtat
    14761 aagaaagttt attttatccc ttttaaccta tacatttacc ataaaaaatg aaaaatgatt
    14821 aaattttagt cgccaattaa agaatttttt ctttaattct attgttacat aaaaattttg
    14881 tggtaatttt gcaaactgaa aaataaattt taaaagataa ttaatcaact tttaaattaa
    14941 aaaaatgaca aaaattgatg taagtaaata cggaatttcg aataatatcg aaatcgtata
    15001 caatccaacg tacgaacagt tgtttcgtgc agaaacagac ccttctaata atggttttga
    15061 aagaggtgta gttaccaatt tgggggcagt agcagttgat accggcatat ttacgggtcg
    15121 ttctcccaaa gacaaatttt ttgtaaaaga cgaagttact gaaaatacca tgtggtggga
    15181 cggcgagatt aacaaaccta ttactgttga agcttggaat gattgtaaaa aattagcaac
    15241 tgaccattta tcaaaggcaa aaaaattata cgtagtggat gctttctgtg gcacaaacaa
    15301 agatacacgt atgaaagtgc gttttattat ggaagtagcc tggcaagcac attttgttac
    15361 caatatgttt attcgtcctt ctcattatga attagcaaat tttggagaac ctgattttac
    15421 tttgatcaat gcttcaaaag taaccgatcc aaattggaaa gaacatggac ttcactcaga
    15481 agtatttgtt ttattcaatc tttcacaaca catggcagtt attggaggca cctggtatgg
    15541 cggcgaaatg aaaaaaggta ttttttcttt aatgaatttc tatcttcctc aaagagggat
    15601 tgcttccatg cactgttctg caaacgtagg agaagaggga gatgtagcta ttttctttgg
    15661 cctttctggt actggaaaaa caactctttc tgctgaccca aaacgttatc tgattggtga
    15721 cgatgaacac ggttgggatg atcacggcat ctttaattat gaaggtggct gctatgcaaa
    15781 agttattgac ttgagcaaag aaaatgaacc cgatatttgg ggagctatca gacgagatgc
    15841 cttgctggaa aatgtaacgg ttttgcctga cgggacgcct gatttttcaa aagctgcatc
    15901 aaaaactgaa aatactcgtg tctcttatcc aatttattat attaacaaga ttgtgttacc
    15961 ttcaaaagct ggacacgcaa gtaaaattat ttatctttcg gctgatgctt atggagtttt
    16021 gcctcctgtt tctattctgg atgaaaaatc agctcaatac cattttcttt gtggatatac
    16081 ttcaaaatta gctggtactg aaagaggtat taatgaacct gttccttctt tctcacctgc
    16141 ttttggtgaa gcttttctca cacttcatcc tactatttat gctaaagtgt tagctgaaaa
    16201 aatgaagaag cacaatgcca aggcttatct ggtaaatact ggctggaatg gtaccgggaa
    16261 acgtatttca atcaaggata cgcgtgctat tattgatgcc atcatcgatg gctcaatcga
    16321 aaaagctcca actacccata ttcctattat gaatctaacc gttccaactt cattaaacaa
    16381 tgtagatcca aaaattctgg atccaagaaa tacatatgcc aattcaggag aatgggaaga
    16441 aaaagcaaaa tcgttggcta aacagtatat taaatatttt gaacaatatt gtgataatgt
    16501 agatgctcgt gttttgcttg cttcaggtcc acaattataa tttttcacaa taaaaatcca
    16561 ctgaaaaaga atgagccctt taaaacaaag actcattctt tttcatttat ttcttgatga
    16621 aagttgagaa aagataatta aacaaaaaaa attctaatga aaatagatat ttgtttttct
    16681 cctgctttat atccttatta ccaaacgtca gaggcaagaa tggtagtaat ggtagatgtt
    16741 tttagagctt ctactacgat atgtgctgct tttgctactg gtgccaatgc cattataccc
    16801 gtggcaacta ttgaagaagc tttgcgatat aaaaatcaag gctggatagt aggagctgaa
    16861 cgaaatgtag aaaaatgtaa ttttgccgat gcaggcaatt cgccgtttga atacacacct
    16921 gaaatggtaa aaggcaaaga aattgttttt actaccacga atgggacaca ggcaattaat
    16981 tcagcttcca gcgaatggga agtaataatt ggttgttttt ctaacctaac agcagttaca
    17041 aacttttgtc tggcaaataa aaaagaggtg ttggttcttt gtgctggatg gaacaatcgc
    17101 tttaatctcg aagatgctgt ttttggagga gctctggctg aacgattgat aaaagaagga
    17161 aattttgagc cttgctcaga tgcagtgata acagctcaat ccctttggca actggctggt
    17221 aatgatcctg aaaacttttt gaaaaataca gaacacgctc accgattatt ttctcatcat
    17281 ttggaagcag attattattt ttgtttacaa acggatacaa cagatgtttt accgtattat
    17341 gataaaactt ctaaaaaatt gcttataaag aaataataaa attataaaaa atttgatttt
    17401 tatagcacct tcctgaaacg gtaaaacagt aagaggctta taattcacct tcctcataaa
    17461 aaatgataga attttcgaaa ttaccttatt catcaggttg tttttctttt ttctgaacgt
    17521 aattagcaac actgtttaaa attgttgttt gcaatgtata tcaaaaagta cgtagcaccg
    17581 atattttggt agccacttat gccagttaat gaactggtat cattataaat tcagataaaa
    17641 atctgaaacc aatgctttaa attccggcgt atattgatgt cggtgagtgg tttcaattac
    17701 caatttactt acacgagtca cagcatcttc ttttgaaaag tgaagcaaca agcgttttgc
    17761 tggaagttca atacgaggat aaacaaaaac caactgctga caccccatgt ttaattccaa
    17821 agcatgcaaa taacatttca atccttcttt tacaggtaga ataaggcaaa tattgccggt
    17881 cggtttcaaa atttgattac ccaattcaat caattctgaa tgagaaagtg tgtcagcatg
    17941 tcgtgcctgt gttcttgaca cagaaggtgc tttcagtgag tttacaaaat aaggtggatt
    18001 tgaaacaatc aaatcatatt tcgaagcagt cttttgaacg tattcttgaa aagatatttc
    18061 agataactga atacgatttc tccatggcga aaaattaata ttttgttgtg cctgaaaaat
    18121 ggcttgttta tcaatatcaa tggcatcaat gaaagcatta cttctttgaa ccagcatcat
    18181 tgaaattaaa cctgtccctg taccgacttc taaaatatgc atagaatttt caagtggtgt
    18241 ccatgctcct aacaaaacgc catcaacacc gactttcatg gcacatttgt cctgaaaaac
    18301 tgtaaatttt ttaaacttga aataaggatt agacatatgt tattgaaacg attttgccat
    18361 tctctttttt actttcgagg cgaaggcttt gaagaagtat ttttttgctt cattctattg
    18421 ctgggactac tttcgacaga ttttctctca tttataggac gataagaagg cgtaatattt
    18481 cttattcctt cggtggtagg tacttgcatg gtggaacgtt cattacgttt gttcagaagc
    18541 tgctgatatt gatctccact taatacttgc tgaatttcta catctttttt cttgatgagt
    18601 tccattactt ctgaacgttt attgcaaagt tgacgttgtt tggcatatcg aagattaatt
    18661 tcataaattt tttttgtttg ttcaagagtc aaattcaatt cttgttcaag cctggcagtt
    18721 tgcttggcgg cttcttgctc aggtgaattt tcaacagcag tttccggcaa ttgagcttgc
    18781 aaataattta caaaacccag cattaaaatt attatacaaa tataattttt agctttcatt
    18841 ccaaaaaaag aaatttttta atagctaatt tggcaggttt taaacaaata ctaaaagcac
    18901 tcattccttc aataaaaaat acaacaacga ttgtgccaca ataaatgaaa acctaaaaaa
    18961 ttctcaatag ataataaaaa atggttcttc atttaagttc ttttattaac tttatatcaa
    19021 tttctcaaag taatttttgt tatatcaact accgacgaaa acattctgta attgtcaaaa
    19081 aatatgaaaa aaattggtat tctttccgat acacacggtt ttttagatga tcgagttttt
    19141 gaatattttg ccgattgtga tgaaatatgg catgcaggcg attggggttc aatggaagta
    19201 gtaaattcat taactcaatt taaacccgtt cggggagtat atggaaacat ggatggttat
    19261 gatgttcgtg ctgtttttcc tgagtataat cgctttctgt gtgaagaagt cgatgtatgg
    19321 ttgacacata taggtacgca acaaggtaaa tatttaccag ccattcaatc tgtttttatt
    19381 catcaacctc ctgatttgtt tgtctgtgga cacagtcata ttttgaaagt gaaatatgat
    19441 aaaaaattcc atttcttgta tgttaatcct ggcgcagaag gtaaatttgg aattcataaa
    19501 atacaaacta tcattcgttt gaagattgac agaaaagaaa ttaaagatct tgaagtcatc
    19561 gaaatcggcg aaaaataagg gtgatttttg gattttcttt taaaaataat atccagtttc
    19621 taaccaaaat gcgtaattta gcatgttttt ttcaaattta gaaaactatt taaaacatat
    19681 ttaataaaga aatgatattt caaatagacg aaacccttgt cagcagcgat ttgataactg
    19741 ataggtttgc atgtgatttg tctttctgca agggaatttg ttgcgttgaa ggtgtttcgg
    19801 gtgctcctct ggagaaaagt gaaattgaaa tcatagaaaa aattttacca atcgtctggg
    19861 acgatttatc tgaaaaagaa agacaaatta ttaaaaatca aggtttttgg tatatcgacg
    19921 aagatggaga tactgtaact tcattagtca agggtaaaga atgtgttttt acttatttcg
    19981 atgcggatgg tacttgtaaa tgtgtctttg aaaaagcttt ttgtgagggt aaaactgatt
    20041 tcccaaaacc tgtttcctgc catttatatc ctgtacgatt gcaaaattat gatgaattta
    20101 tagcggtaaa cgttcatcat tggcatcatt gtctttacgg ttataagcag ggaaaaaatt
    20161 tacaaatacc gctttaccgt tttctgaagg acgctttgat tcgtcgcttt ggcgagaatt
    20221 ggtatggtca gttagaaaaa attgctcagg aagaagaatc aaaaaaaata taaaatatca
    20281 aatttattca tacaacaaaa aattataatt taagcatgaa aacccctaaa gttgaaatta
    20341 ttacaattgg agatgaaatt ttaattggtc aaattgttga taccaattca gcctggatag
    20401 cccaagaatt aaataaaata ggctttgaag ttttccaaat aacctccgta catgatgagg
    20461 aaaaacaaat tatagatgct ttacatgccg ctatgaaaca tgtggatatt gtactgatga
    20521 ctggtggatt aggaccgact aatgatgaca tcacaaaaaa gactttgtgt aaatatttta
    20581 atacgaaatt aatatttgat tcttccattt tggaaaatat taaaaaggtg tttgcttctc
    20641 gaccagaatt tgttattaat gatttgactc gttcacaagc ttttgtccca gaaaattgcg
    20701 aagtaattca aaacaccgta ggtactgctc ctattatgtg gttcgaaaaa gataaaaaaa
    20761 taattgtttc catgccaggt gttccttacg aaatgaaaga agccatgagc agagaagtga
    20821 tgcctaagtt gcaagcttat tttaaaacgc ctaaaattat acacaagact gtccaggttt
    20881 tcggatattc tgaatcggat ttagctatta aaattgcaga ctgggaaaaa aatttgcccg
    20941 aaaacattca tttagcctat ttacctaatt atggaattgt aaagttgcgg ttgagtggca
    21001 cttcagataa tcttctttct ctggaatttt cacttaatca gcaaattgat gctttaacac
    21061 tgatattggg aaatgccatt gttgcttatg aggaccttcc catggaacag gttgttgcaa
    21121 aattattgct ttcgcaagaa aaaacggtag taacggctga aagttgtacc ggaggaaaca
    21181 ttgctcatcg gtttacttct cattcaggaa gttccaatta ttttaaaggc tccgttgtag
    21241 cttacgataa tgaagtaaaa atcaaccttt tgggagtttc aactgacgat cttgaaaaat
    21301 ttggagctgt tagccagcca gtggttgaac aaatggctgc tggcgttcgt cgcttattaa
    21361 atgctgatat tgctgtagct acttctggaa tagcaggacc tacaggtgga agtccagaaa
    21421 aacctgttgg cacagtttgg atagctgctt gttcagaaga aaaattagtc agtaaacttt
    21481 ttcattttag caatacgcga gaacaaaata tcgaaagagc tactcaagca gcgttagtaa
    21541 tgataaagga actattataa gataatttca aagaaaagaa aagaaaagaa aagaaaagac
    21601 tgttaaattg ttgtttaaca gtcttttata attttgtcgg gatgactgga ttcgaaccag
    21661 cgaccacacg ccccccagac gcgtactcta aaccggactg agctacatcc cgattatttg
    21721 aaaggaatac aaaaataacc aaatttttta atttaaacga aatctatttt tctgttttct
    21781 acatttcatc gaaaacaaca attcattgat tggtgttagt ggaaattatt tttcaaaatt
    21841 agtattcatc ccagcttttt attttaatat cttccaattt tatattacaa aaagcaaaaa
    21901 taaaagcact ggcaagtcgt gcgtttgtta ataaagggat attataatcg atagcactgc
    21961 gacgtatgac ataatcattt gccaattcac gttgagttac attctttgga atattaatta
    22021 ccaaatcaaa ttttctttgt gcaatcatat ccttgacatt atactcgcct tcctcatcag
    22081 gccacaggac tactttactt tcaataccat tttcagctaa aaattgatgg cttcctcttg
    22141 tagcataaat ctgataacct tttttataca gtaacctgca agcatcaagc aaatccactt
    22201 ttgattttgg ttcacctgaa gaaatcagaa tatttttctc cggaatttta aaaccaactg
    22261 ataacaaagc ttttaaaact gcttctgaaa aatcatcacc taagcagcca acttctcccg
    22321 ttgaagccat atctactcct aaaatggggt cagcttgctg caaacgtgca aatgaaaatt
    22381 gagaagcttt aataccaaca taatctaagt caaaagccga tttttcaggt ttttccacag
    22441 gaagtcccag cataattctg gttgctaaat caatgaaatt tatttttaac accttcgaaa
    22501 cgaaaggaaa acttcgtgaa gaacgcaaat tacattcaat tacttttata taattatcac
    22561 gcgccaaaaa ctgaatgtta aatggtcctg aaatttgcaa ggaatgagca atttgtcgag
    22621 cgatattttt aatcctgcga atggtttcta tataaatttt ttgtggtgga aattgagtgg
    22681 tggcatcgcc tgaatgcaca ccagcaaatt ctatgtgttc tgaaatagcg taagcaataa
    22741 tttcaccctt attggcaact gcatcgattt ctatttcctt gcaacgttcc aaaaattctg
    22801 aaactactac tggatgcttt ttagaaactt ccgttgcaac ttttaagaaa ttttcaagtt
    22861 gcgaatcatc ataacaaaca ttcatagcag cacccgataa tacataagaa ggacgaacca
    22921 atacggggta tccaatccga ttcacaaatt ctttaacatc ttcaaaagta gtcaattctt
    22981 tccatcgtgg ctgatcaatt cccaattgat caagcatagc agaaaattta tgacgatctt
    23041 ctgccatatc tataaattca gcttgcgtac ctaatatttt tacatttgcc ttagctaatt
    23101 tcaaagccag attgttaggt atttgcccgc cagttgaaac aattacacct tttggatttt
    23161 ccagctcaat aatatccatt acccgttcaa aagtcaattc atcaaaatac aatcgatcgc
    23221 acatgtcata atcagttgag acggtttcag gattatagtt gatcatcaca gaacggtaac
    23281 cttctttttt gatagtcatc agagcattta cactacacca atcaaattcc acagaagaac
    23341 cgattcggta agctcctgaa ccaagaacta taactgaaag ctggtcgttt tcatactgaa
    23401 tatcatgagt agatccatta tatgttaaat acaagtagtt agtttgtgca ggatattcag
    23461 ccgccagcgt atcaatttgt ttgacaacag gtaaaatatt tcgacttttc cgatattcac
    23521 gcgtcacttt tatcatatca tccatttcat cgctactgca tttaagaaca agccgtgtaa
    23581 tctgaaaatc agaaaaacca gcttttttag cttcatacaa taacttatca ggcagagatg
    23641 ataaagaatt ataacccatt aattcctttt ccaaatcaat aatatgtttt aatttgtaaa
    23701 gaaaccatcg gtctattttt gttaactcgt acaatcgatc aatagaataa ccctcgtgta
    23761 aggcttgtgc aagataaaat acacgtttat cagtcggttg agaaagagca tcatcaatat
    23821 catctacaaa ataatctcta ttggcaacaa atccgtgcat tccaagtcct accattcgca
    23881 atcctttttg aaagatttct tcaaatgaac gcccaattga cataacttcc cccaccgatt
    23941 tcatactgct gcctaataaa tgagaaacac cattaaattt attcaaatcc caacgtggaa
    24001 ttttgcaaac aacataatct accgctggtt caaaaaaagc agaagtaact ttggtgatgg
    24061 aattttttaa ctctgctaaa ccatatccca aacctaattt tgtagcaata aaagccagcg
    24121 gataacccgt tgcttttgaa gccaatgctg aagaacgact caaccgagca ttcacttcaa
    24181 tgacacgata atcttcagaa ttcggatcga gagcatattg tatgttacat tcaccaatga
    24241 taccaatgtg acgaataatt ttgatagaca attcccgtaa tttataatat tcactgctgg
    24301 tcaacgtctg tgaaggagca actactacac tctcgcctgt atgaataccc agtggatcga
    24361 aattttccat gctacaaact gtgatacaat tatcatatct atcacgaact acttcatatt
    24421 caatttcttt ccagcctttt aaggattttt caattaaaac ctgtggtgaa taagaaaaag
    24481 ctttttcgac aacagatttt agttcctcat cattattgca aaaaccactt cctaatcctc
    24541 ccaaagcata agcggctcgc acaataatag gataacccac tttatgtgcg gcttcgtaag
    24601 cttcattcaa atgagtaact gcttgacttt gaatatattt tacatttatt tcagataaac
    24661 gctgattgaa aatttctctg tcttccgtat caataatagc ttgaactggt gttcctaata
    24721 ctcgcacctt atatttttca aaaatattat tttggtaaag ggcgactcca caatttaatg
    24781 ctgtttgtcc accaaaagac agaaagatac catcaggtcg ttctttttca attacacgtt
    24841 ctacaaaata aggtgtgata ggcaaaaaat atactttatc agcaatacct tcagaagttt
    24901 gtacggtagc aatattggga ttaattaata ctgtttgaac gccttcttct tttaaagctt
    24961 tcaatgcttg agagccggaa tagtcaaatt ctcctgcttc tccaattttc aacgctcctg
    25021 agcctaatac cagtactttc tttatttcac ctttttcagg accattataa gggtgtttct
    25081 taacttgtaa taaacttaat tctttttcaa ttaattcagg aataattttg tttttttcag
    25141 tcaatgtttt aataaaaaca tcaaataaaa attccgtatc agttgggcct gccgaagctt
    25201 caggatgaaa ttgcacagaa aaccagggtt tagtagtatg ataaatacct tcattagtac
    25261 catcattaat attcaaaaat aatggtgccc aatttttatc aagtgtttga ttgttaatag
    25321 caaaaccgtg attttgagaa gtaataaaag catgttgtgt gcctaccagt tgaactggtt
    25381 gattactact gcggtgacca tattttaatt tgtatcgttg agctccacct gctatagcca
    25441 atagttgatt ccccatacaa ataccaaaaa taggcttgtc gttctccata gcaacacata
    25501 tatgttcaat agtagaagaa caatgtaatg gatcgcccgg gccattcgaa ataaacaaag
    25561 catcaaattc taactgatta aaatcataat cccatggaac gcgaataatg gtcgtatcac
    25621 gtttaagcaa acaacgtaaa atgttatgtt tcactccaca atctaccaac acaatccgat
    25681 gttttccatt accataagtt attacttctt tacaacttac ttttgctact tgattttctt
    25741 tatcaggatc aataaaattt atatcttctc cgtcaggaaa aactaatttt cctttcatag
    25801 tccctttttc acgaaccaac ttggttaatt cacgtgtgtc tatgtcaaaa atagctggaa
    25861 tttgattttc tttcagccat tcacttaaac tttttttagc attccagtgg ctatattgaa
    25921 acgaaaaatc ggaaacaatt aagcctgctg cctgtattct ttccgattca taaaaagttg
    25981 atattccatt gtctaaacaa tcctgaggaa ctccataatt accaataaga ggataagtaa
    26041 atactaataa ttgaccacta taagaaggat ctgttaaact ttcaggataa cctaccattg
    26101 aagtattaaa aaccacttct cctgctactt gcttttcata accgaaagat ttacctttaa
    26161 aaacagtccc atcttctaaa actaagtgaa tgggtttata ttcttgcata aaataaatga
    26221 ttaactaatt ttgttaagga actcagagta tataattcct tttgctgaat tttaatgatc
    26281 acttttgcac atttttatta tgttcaaatt tttacaaaat tactgctttt tctttttttg
    26341 tacaagcagt atgataaaaa ctcactaaga attttatatc acaaactttg aaaattttta
    26401 tccgagaata attttattaa gttaaaccat atcagtattt tgaaaaaaca gataagaagt
    26461 taaaaaaatt atctttatac ttaaaaatat ctatatttat ttatctattt gaagtcagat
    26521 tcatttaatt acttgaattt acgctttaat ttatcttgat tttgtttttt ataagataat
    26581 aaggtttgat taatttgttt gttataagcc attaaggttt ttttgaaaaa taaggtttct
    26641 ttttaaatga ctttccactt ttcaataaaa aaattactgt tctttaatta ctatttgcaa
    26701 gtttttagca taaaaatttc tattgttttt agtttctgcc tttaaaacct tatcattttt
    26761 ttaaccttaa taacattgaa atttcattcc ttttataaac cttattacct tatttataac
    26821 gacttaatgt ttacttttca atcttaatct tgttttttta taagatggta aggcttagtt
    26881 atggatttat ttgtattcat tgaggttttt agcaggataa aattccaatt gacaagtatt
    26941 ttcattagcg tttatgcttt taaaatataa tgaggttttg atgtaaaaaa catcttcatt
    27001 cattataaaa aatagaaaag gtttattact ttaagtaata aacctcttta aatcaaaatt
    27061 ctaagatttt ttaatcttta aatttagctg ataaatattc tctattcaaa cgagcgatat
    27121 ttgaaataga aacacttttc ggacattctg cttcacaagc tcgtgtgtta gtacagtttc
    27181 caaatccaag ttcttccatt ttagccacca ttgctttagc tcgcttagct gcctctactt
    27241 tcccctgagg taaaagtgcc aaatgactta ccttggcagc aacaaacaac atggcagaag
    27301 catttttaca agcagctaca cacgcgccac agccgatgca agtagcagca tccattgctt
    27361 catcagcacg atccttagga ataagaatat tattggcatc aggagctcct cctgtgttta
    27421 ttgaaataaa acctccagct tgaatgattt tatcaaaagc acttcgatca acaatcaaat
    27481 ctttcacaac aggaaaagca gccgacctcc atggttcaac gacgatagta tcaccatctt
    27541 taaattttcg catgtgaagc tgacaagtag taacctctgt atcaggaccg tgaggatgtc
    27601 cattgatgta caagccacac atgccacaaa ttccttctcg acagtcatga tcaaaagcaa
    27661 taggctcaat atgttcttta attaattttt catttaatat atccaacatt tccaaaaaag
    27721 aactatcggt tggaacattt tctaaattat aagtttcaaa ttttccctta gctttggcac
    27781 tggcttgccg ccatattttc aaggtaatat ttattgtttt ctccataaaa actgatattt
    27841 tttcttaaaa gaatttctgt ttcttattct ttataatttc tttgttgaag ctgaataaat
    27901 tcaaatttga gtgattcttt caacaaaatg ggttctgtat cttctcctgt atatttccaa
    27961 caagaaacaa atgaaaattt ctcatcatta cgcagcgctt ctccctcagg agtttgatat
    28021 tcttctcgga aatgcccccc acaagattcc tcacgcatca atgcatcacg tgcaattaat
    28081 tctccaatat caataaaatc ggctaaacgc aaagcttttg ccaactcatt attaaaatca
    28141 tactcatctc ctggaacata aacattattc caaaactcct ttcgaatatt tttgattttt
    28201 tctaatgcaa tttgaagtcc ttctttattt ctcgacattc ctactaaatc ccacattatt
    28261 agtcccaact cacgatgaat agaatcaacg gtacgttgtc cttttatgtt tattactttt
    28321 ttaatccttt tagtaacatt tttttctgct tcatcaaatt caggcaaatc ggtgtccata
    28381 cgtggaacct gaatttgatc agctaaatag ttctgtatag tgtaaggcaa gatgaaatag
    28441 ccatcggcaa gaccttgcat taaagccgaa gctcccaaac gattagcacc gtgatctgaa
    28501 aaatttgctt caccaataca aaacaaccct tttatcgaag tttgaagttc ataatcaacc
    28561 caaatgccac ccatcgtata atgaatagca ggataaatca tcatgggtgt tttataagga
    28621 ttttcatcaa caattttttc atacatctga aaaagattac cataacgttc gcgaattacc
    28681 tcttcgccca aacgttgaat agcatctgaa aaatcaagat aaactgccaa acccgtattc
    28741 ccaactccat agccagcatc gcaacgttct ttagcagcac gcgaagcaat atcacgcgga
    28801 acaagatttc caaaagcagg ataacgacgc tccaagtaat aatcgcggtc ttcttctttc
    28861 aaatcggttg gttttagttt cccagctcga atagcttctg catcttcttt tcttttgggt
    28921 acccaaatac gtccatcatt tcgtaacgat tcagacatta aagtaagttt tgactgataa
    28981 tcaccatgca aaggaatgca cgtaggatga atctgcacaa aagcaggatt ggcaaaataa
    29041 gcaccttttc tatacatctg cattacagcg gaaccatttg aagccattgc attggtagaa
    29101 agaaaatacg tatttccata acctccggaa gcaattacta ccgcatgggc aaaataacgt
    29161 tcaattcttc ctgtaattaa attacgagca atgattccgc gagcacgccc ctcaataatg
    29221 accacatcta acatttcata acgaggaaat atttttacag taccattggc aatttgacga
    29281 ctcaaagccg aataagcacc taacaataat tgttgtcctg tttgtccttt cgcataaaaa
    29341 gtacgggaaa cctgagcacc accaaacgac cgattttcaa gtaatcctcc atattcacga
    29401 gcaaaaggta caccctgtgc cacacattga tcaataatat tattggaaac ttcagctaaa
    29461 cgataaacat tcgcttcacg cgaacgaaaa tcaccgcctt ttatcgtatc ataaaaaagt
    29521 cgaaagacag aatcaccatc attttgataa tttttggctg cattaatccc cccttgagct
    29581 gctatagaat gtgcacgacg tggagaatcc tgaatacaaa aatttagcac attaaatcct
    29641 aattcaccca acgaagcagc cgcagatgct cctgccaaac ccgtccctac tacaattaca
    29701 tctaacttac gtttgttggc aggattcacc agtttttgat gagctttgta gttggtccat
    29761 ttttcagcca aaggtccttc tggaatatgg gtatcaagaa taaaattttc accttcctga
    29821 gttctttcgt tatttttcat atttggattt gtattgataa gcttttctct gatttttaaa
    29881 aaatttattg taatatattt actatcaaat aataaatagg aacagacata aacattaata
    29941 caataatagt agaaactata ttgccaatta cttttatgcg gggtaaccat tttttattat
    30001 ttaatcctaa agaatgtaaa gcgctccaaa cgccatgtgt taaatgcaac cacaaagcca
    30061 ccaaccaaat gatataaaga gcacaataaa tccaatggct aaaaagtgca cttacataag
    30121 cagcaccatt ctgtggatca aaagctcctg tatgtattcc tgataattca gcaaactgca
    30181 tcttaaacca gaaattatat aaatgtaaaa caataaagcc caatattatt acccctaata
    30241 tcagcatgtg ctttgatgac cactccacat cagttggggt ggcttttacc gcataacgaa
    30301 cttctccacg agcacgaaaa ttcaaaaaag ataaatataa agcatacaaa atatgcacga
    30361 caaaacccaa tgccaaaaca agtgagccaa ttacagcata ccaatttgct cccaaaaaag
    30421 cacatatttg attataactt tcaggagaaa taatgaccac aatattcata caactatgga
    30481 acaataaaaa aagcaccaga aaaattcctg aaatactcat gataaatttt cttccgatgg
    30541 gagaatcaat tagccacata aattttattt ttacttgatt tgcaattaat tttcagaaaa
    30601 tcaaacaatt aaagctatta tagctttctc ttaaatttgc cacaaaagta ctactttgaa
    30661 cgtaaagaaa caaaaaatta aagaaatttt tctttaatgt gtttttttat tgttgttttg
    30721 attcgccttg tttgaaagaa atcacaccca attaatactt ttattttaga aatcatttct
    30781 atgtgacttt gccccaaccc tacttgcttt tttattattt tcagtagaga cgccatatat
    30841 gacgtctcta ctgaaatgcg ttgaatgaca gttgattatt taaaataaag gagtgctggt
    30901 aatttttttg aacaacgatg gctgcaccga aacaaaaacg aattgaataa aattgaataa
    30961 atgaatatgg gaattggtag agacgccgca tgcaacgtat tctatggtta attccaaatt
    31021 attttagtta aaaaaacata tttttaagca cattccctaa atgggttaag ataattttcc
    31081 acataaggtg ttttgtgttt tatgacagca ctctacaaga aaaattgacg ttcgtggcta
    31141 aaaaattaac cgtcagtcgg atttgcaatc cgactggatt atttgcggat tttaaatccg
    31201 ccattaaacc agcaaaggat tataaatcct ctttgacaga gtttaagaaa aatgaatggt
    31261 taatgcatga ataatcaagc ataaaatgat taaattgcat ttcttccttt cctgttttgc
    31321 aaaccataaa ccgaaggaag tgacatccgt taaattaccc tactacttcc ccaaaaagcg
    31381 tggcagaaga aaaatcagtt attttaactt cgacagtttc acctattttt ctctcttttc
    31441 gggggaaaat tactacctta ttttgtgaag tacgtccaaa aaatttttct cgtgaccgtt
    31501 ttgaaaaacc ttctaccagc acttcaaaaa ttttgcccac atcttttcga ttactttgaa
    31561 gtgaaagatc cttttgcaag ccgataattt catttaatcg ttgaagtttt acttcttcac
    31621 tgatgttgtc aggtaaattt ttagcagcat atgttccggg tcgttcagaa tatttaaacg
    31681 taaatgccat atcaaatttt acttcctcca tcaatgacaa agtctgctga aaatcttctt
    31741 ctgtttcatt agggaaacca caaaacacat ctgtcccaat ggctgcatca ggtaaaatgc
    31801 gacgaataga atcaatcttt tccaaataac tctcgcgtgt atatttgcga ttcatcagtt
    31861 ttaaaatttt attactcccc gattgtacag gtaaatgaat ataatgacat aaatttggat
    31921 attgagctat tacctccaat gtacggtcac tcatgtcttt tggatgagaa gtagtgaaac
    31981 gaaatcgcat ctcaggtgct ctttctgcaa caatctttaa taaatcagga aattctatca
    32041 ttttttcttg atattcaaac tgataagagt ttacattttg tcccaacaaa gtcacttcct
    32101 taaaatttct tgctcttaaa tcatccagct catgcaaaat actttctaca tctctacttc
    32161 gttcccgacc acgggtataa ggcacaatac aataagaaca aaaattattg caccctctac
    32221 taatggatat aaaaccagaa acagaatttc ccattcgtaa aggaattatt tcgcgataag
    32281 tttcagtagt ggaaagtaat acatttactg ctttttctcc attttctact gcagatatta
    32341 aattaggtaa atctaaatag gcatcaggac ctactactaa atctactcca taattggtta
    32401 tcaaatcttc ttttattcgc tctgccatac accccaaaac tcctattatc aacttcctat
    32461 ttcgacgttt aaacgattga aaatatttta atcgttctat cactcttcgt tctgcattgt
    32521 cacggataga gcaagtattt acaaaaatgg cgtcagcttc attcaattgt gtggtcagtt
    32581 cataaccatc cattttcata atagcagcaa ctacttcgct atcagccaca ttcatctgac
    32641 agccatacgt ttcaatatat aattttttat tttgtgattc ttttgaaagt tcaagagcca
    32701 aaattccgtt cattttcatt tttcatattt tttccaaata caaagttaca tcaataaatt
    32761 gaaaattacg agataaagtg cttaacgtta tttaactatt ttaatatagt ccttatcttg
    32821 ttgaaaatgc ttaaattaca attatttttt aataaaaatc agaaaaaggc tgcaaacgtt
    32881 tacagcttca agaaaatatc tttactttgt agagtaaaca aacaaataaa tttttcatag
    32941 ttaaggttta ggttaaatgg ggcaatgggc aactgggaag ttgttcatta ctagtagcat
    33001 atcggcatac ttttctgagt agcaaggtga aacttctttt ttacaaattt agaatttcct
    33061 gtatttaatt gataatcaat aatatataat acatagaaac ccttgatagg caatatcttt
    33121 atagtaaatg aataacctcg aggcaagcct cgaagaattt ttttgattaa attttcgcaa
    33181 taaaaatagt gtcctatcag gaataaaatg ttagtaaaga aattgtttac agcaaaaagt
    33241 aaaaaggaaa aaatcattat gattatccgg gtaagactca gtatttcttt gtattagaat
    33301 gaatttaact tcaccaagct acactgagaa aaaatcgttt tttctcaaga caaaaaagtt
    33361 attcggttat agtgcaaagc ctcaaattga aaaccacacg atatccttgt ggaggaacca
    33421 aaagaaaaaa catttctttt cttctcaaaa aatcaccaaa tcttgtagaa ttttctaaaa
    33481 atcttgtata tttgtaataa ataaactttt aaaatgacaa ccaaattatt gtcatgcata
    33541 ttaccgtgat gtcggttgca ataactaaag ggaattataa cctcaacatg aaatgatttt
    33601 tttcaagcga agcaaccatg cctactgcca tagaatttaa caacatttcc aaacaatacc
    33661 gcctgggact ggtttctacc cgtacactga gccacgacct gaaccgctgg tggaaaacac
    33721 aagtactccg gaaggaagat ccttacctga aaataggtga agtaaacgac cgcgcccaaa
    33781 aaagggaaag tgactatgtt tgggcactga aggatattaa cctgaaagtg gaacaaggcg
    33841 aagtgctggg cattatcggt aaaaatggtg ctggcaaatc tactctcttg aaaattcttt
    33901 ccaaagtgac agctcctacc actggcacga ttaaagtgaa tggcagaatt gcttccctgc
    33961 tcgaagtggg cacaggtttt catcccgaaa tgacgggacg ggaaaatatt tacatgaacg
    34021 gcgccatact gggcatgacc aaaaaggaaa tcgaccgcaa actcgacgaa attgtggatt
    34081 tctcgggtgt ggaacgttat atcgatacgc cggtgaaacg ctactccagc ggtatgacgg
    34141 tgcgattggg atttgccatt gccgcccatc tggaaccgga aattctggtg gtggacgaag
    34201 tgctggccgt aggcgatgct gaatttcaga aaaaagccat tggcaaaatg caggacgttt
    34261 cccgcggcga tggccgaacc gtgctgtttg tgagtcataa tatggaaagt attcaaaatt
    34321 tatgcaacaa tggtatttta ttgggaaacg gaagtattat taaatcagga aagattgaag
    34381 atgtggttaa ttattatatt tccggtaatc tggcatttgc agaagatgta aattcaataa
    34441 acaatattga taaatacaga ccgaaaggca ccacaaaaga agtagaattt ttatacctta
    34501 aaagagtaag taattaccga atcgatgtga acgatccact tattttcaaa atgggtttga
    34561 aaaagaatgc aaaagtaaaa aaaattcaat taatggcttt gattgatgat atagccaatg
    34621 gaggaaacag agttgggatg gctttttccg aagaaatatt attacccgag cacaaaaatg
    34681 aatttgaagt ggaatttacc attcagcatc ataatttaac caaaagtaaa tatatcatgg
    34741 atttttggat aggaattggt gatgtaactt ctacaattaa atactatgac atgttgtacg
    34801 ataccttaac ctttgaaata aatacctgca atggtaaata tatcaacgaa tggcataatt
    34861 actggggaca aaattgttac acctctgtaa acgttgtttt aaaatgaaaa aattagctgt
    34921 tattggtgca agttatttgc aaaagccac
//
LOCUS       DAOSAS010000004        26988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_1302_length_26988_cov_12.462407, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000004 DAOSAS010000000
VERSION     DAOSAS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 26988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26988
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1302_length_26988_cov_12.462407"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..119
                     /locus_tag="PLR72_00415"
                     /pseudo
     CDS             1..119
                     /locus_tag="PLR72_00415"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            135..416
                     /locus_tag="PLR72_00420"
     CDS             135..416
                     /locus_tag="PLR72_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35710.1"
                     /translation="MKIHGKEVEKKSKLVLSDTTVQQNNTTFPTDAKLCKKVIDKCNT
                     IAEKEGIEQRRRYTRESKQLLRDTYNGTHPKHARKAKKAKKRLKMITNR"
     gene            438..1082
                     /locus_tag="PLR72_00425"
     CDS             438..1082
                     /locus_tag="PLR72_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOK35711.1"
                     /translation="MSEEQRRRYEKELELYKRAVNQRKEDKDKIYSLHKPFTHCISKG
                     KPHKPYEFGNKVGLITTGKKGKKIITAIRAFTENPFDGHTIDPLLEQMKGNDLKLPKE
                     LVYDRGGRGKKEIRGVKIITPGKPRASDTAYQKQQKRRKFRARADIEPIIGHLKKDFR
                     MEQNYLMGEEGIQINALLAASAWNLKKMMEKLKEEFFCLIFRLLFRQNFYNLAT"
     gene            complement(1289..1375)
                     /locus_tag="PLR72_00430"
     tRNA            complement(1289..1375)
                     /locus_tag="PLR72_00430"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1339..1341),aa:Ser,seq:cga)
     gene            complement(1457..4048)
                     /gene="glgP"
                     /locus_tag="PLR72_00435"
     CDS             complement(1457..4048)
                     /gene="glgP"
                     /locus_tag="PLR72_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOK35712.1"
                     /translation="MEIKMNSVNEPIWKDVHVKSYLPDGLKKLEELAYNICWVWKSDI
                     RHLFREIDIEAWQEAGFNPVQLLNNLSYDKLVETEKNSEFINKLDVLYNDFKNYMGRP
                     FSPDKPSVAYFSMEYGLTEVLKLYSGGLGILAGDYLKEASDYGVDLTAIGLLYRYGYF
                     TQSLSVNGEQIEIYDPQNFNLLPITQLKDKNNQPLLIEVPFPGRTVYANLWKIAIGRI
                     NLYLLDTDNELNSPEDKSITYQLYGGDWENRLKQEIMLGIGGILALKKLGIEKDVYHC
                     NEGHAALINVQRLIDLINDKNLSFYEALEVVRSSSLYTVHTPVPAGHDRFDEEMFSKY
                     MKMYAQKLKINWQDFMNLGREKPNDPNDKFSMSVFACYTCQEVNGVSWLHGKVSQEMF
                     SPLWPGYFPEELHVGYVTNGVHLPTWTTSEWKSVYEKYFGQEFYDDQSNVKIWQGIYE
                     IPDQVIWDSHLKMKKKLIEFIRTQFPEKWLKTQGDPSRIMTILNSLNPNALIIGFGRR
                     FATYKRAHLLFMDLGRLEKIVNDPQRPVQFIFAGKAHPADQGGKDLIKRIVEISRMPQ
                     FLGKIIFLENYDISMAKRLISGVDIWLNTPTRPLEASGTSGQKAQMNGVLNFSVLDGW
                     WIEGYVKDAGWAITEKRTYENEQYQDQLDAATIYSMLENEIIPLYFAKNAKGISPEWI
                     QYIKNSIALITPRFTTKRMIDNYIERFYSKLSNRLATLKKDNYTKAKELAAWKLKIAQ
                     GWDNIEVVSMDIPEKLFQNPQVSDFYQMNIVIDVKSINDEGIGIELVVTKMDQNQKYK
                     LYDVEEFKLVKADGSTLYYALDYRLNMSGTFKFGFRMFPKNIDLPHRQDLCYVRWLSY
                     DEFLLKT"
     gene            complement(4299..5948)
                     /locus_tag="PLR72_00440"
     CDS             complement(4299..5948)
                     /locus_tag="PLR72_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOK35713.1"
                     /translation="MLIPEYVFEVSWEVCNKVGGIYTVLSTHAATLQKEYNEKLIFIG
                     PDLWLQKENPLFEEDQNLYTDWKFFHHKQYHLDIRIGRWNVPGKPLTILVDFMPLMPQ
                     RNEIYAQVWLNTGVNSLPAYGDYFECSMFGYAAGMIIESYYHFFHLENQQKVVGHFHE
                     WQTAFGIFYLKQHMPQVATVFTTHATTVGRSIATNNKPLYDYLNEYNGDQMSYELNVV
                     SKHSAEKTAAHLADCFTTVSEITAKECAQLLEKKPDVVTPNGFEDDFVPSKEKFQLKR
                     QEARNILLKVSETLLGYKLKKDVLFVCTAGRYEFKNKGIDVFIESLRRLLEHSNLNKE
                     IVAFLMIPAHISGPRADLLACLKHSISKITSWNRFTTHELYDYQNDNIMSAIRWFHFT
                     NQASQKIKVIFVPSYLNGFDGIFNKSYYELLIGMDLTVFPSYYEPWGYTPLESVAFSV
                     PTITTDVSGFGRWVAQQPQGIENGVGIVHRSDYNQYEVSLNIAEQVSHFSGYTEAQVE
                     IIRKRAATIAEKALWEHFIQYYYEAYEVALTHRNDRLSQKS"
     gene            complement(6186..6794)
                     /locus_tag="PLR72_00445"
     CDS             complement(6186..6794)
                     /locus_tag="PLR72_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOK35714.1"
                     /translation="MNKFLLVLNAILCIAVIVLFILFFNLKTNKEERKIITTTEALHT
                     NQLPIAYINIDSLLLHYHFAKDANETLLKKQEDSRLTINMKARELQNEMNEFQKKLEN
                     NAFLSRERAEQEQSRLIKKQQDLQKLDAQLSQKLLEEQQKMSEQLRDSINAFLKEFNK
                     DKKYHIILSNTSNDNILYAVDGYDITSEVIKQLNQRCSQQKK"
     gene            complement(7746..7910)
                     /locus_tag="PLR72_00450"
     CDS             complement(7746..7910)
                     /locus_tag="PLR72_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35715.1"
                     /translation="MEIFNLSVVLPQLVASFKMGEVINMAANKSIIFAICTITLTISG
                     LLWFFVKNEK"
     gene            8433..8615
                     /locus_tag="PLR72_00455"
     CDS             8433..8615
                     /locus_tag="PLR72_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HOK35716.1"
                     /translation="MKDILNFLGAIIVLIGVCILGVYHFAGINSNTLLAIAGLLMIIG
                     FFTFLFMNKKSLKKDF"
     gene            complement(8710..11526)
                     /locus_tag="PLR72_00460"
     CDS             complement(8710..11526)
                     /locus_tag="PLR72_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOK35717.1"
                     /translation="MSDEEIFEEENENILSTDEEELNSESFDTGHSTYQVPKITDESV
                     KHHLSGMFQNWFLDYASYVILERAVPDIHDGLKPVQRRILHSMKEMDDGRYNKVANIV
                     GQTMQYHPHGDASITEALVQLGQKDLLIDCQGNWGNILTGDAAAAPRYIEARLSKFAL
                     ETVFNPKITEWKPSYDGRKKEPVTLPVKFPLLLALGCEGIAVGLNSKILPHNFNELLD
                     ACIAYLKGEEFVLYPDFQTGGLIDISRYNDGERGGVVKIRAKINKIDNKTLAITEIPY
                     STNTSKLIDSILKANDKGKIKIRKIDDNTSSNVEILVYLQPGTSSDKTIDALYAFTDC
                     EISISPNCCVIHDKKPRFMSVSEMLCVSCEDTKNLLEAELKIRKKELEEQFFFISLEK
                     IFIENRIYKEKGFEDSTSHEVAIQYIDKQLEPFKANLIREVTREDILKLLEIKMVRIT
                     KFDADKANNTLIEIQKKIEETDYQLSHLIDYTIAWYESLKAKYGKKYPRRTEIRNFET
                     IEAKKVIEANEKLYVNREEGFIGTSLKKDEFVCNCSEIDDIIVFFKDGKYKVVKVAEK
                     MFVGKNILYINVFKKNDKRTVYNVVYRDGKTGPYYIKRFAVNSITRDKEYDLTQGKPG
                     SKIVYFSANPNAEAEIIRVTLKPKPRLQKLVFEKDFSEIAIKGRQTRGSILTKNEIHK
                     ITLKQKGGSTLGGREVWFDHDVLRLNYDKRGEYLGEFQNDDLILVITQQGEYYTTDFD
                     VNNHYENNILRLEKFEEDKIWSAAIFDADQNHYYLKRFQLSPSQKKVSFIGENPESRL
                     ILLSDKDFPRFEVIFGGKDAYRKPLVIEVSEFISAKSYKAKGKRITTHEIEIINELEP
                     LHTKQEIDTEQVSDYIEIEENGNDRNEESEILEKNFTSLEEASEIEKEESTMIFSEKD
                     SILPDELVNDKKNHDETSQQLSLF"
     gene            complement(11555..12283)
                     /locus_tag="PLR72_00465"
     CDS             complement(11555..12283)
                     /locus_tag="PLR72_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOK35718.1"
                     /translation="METWTDRTELLIGEKGLKILQDSSVLIVGIGGVGGMATEMICRA
                     GIGKITLIDGDIVNASNINRQIIALTSTLYRPKVEVMAERLSQINPELKLIIHHKWLD
                     FSNTISMLEENHFDFVIDAIDTLMSKVFLIKSCVERNIHIISSMGAGGKLNPSKVMIK
                     DISETTMCSLARAVRQQLAKIGIKKGVPVVYSTEPVIKKSIIPVANEANKKSTIGSIS
                     YMPAVFGCYLTSFVIRELLNKSRE"
     gene            complement(12453..14144)
                     /locus_tag="PLR72_00470"
     CDS             complement(12453..14144)
                     /locus_tag="PLR72_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOK35719.1"
                     /translation="MIYKEEPSKRNLNFIEAIVEQDLKEGKNGGRIQTRFPPEPNGYL
                     HIGHAKAICLDFGIAEKYGGICNLRFDDTNPLKEDVEYVDSIMEDIQWLGFQWKNVYY
                     ASDYFQKLWDLAVFFIKKELAYVDEQSAEIIAKQKGTPTIPGIESPYRNRPVEENLEL
                     FYKMSSGEIPEGAMVLRAKIDMSSPNMHMRDPIMYRIITSHPHHRTGWKWKVYPMYDF
                     AHGQCDYFEGVTHSLCTLEFEVHRPLYDWFIDQYQDSDYRPRQIEFNRLNLTYTILSK
                     RKLLQLVQEGLVNGWDDPRMPTLCGLRRRGYTPEAIRNFINKIGYTKFDALNDIGLLE
                     YSARENLNKIATRVSAVLYPVKLIITNYPDNEIEILQTPNNPEDPSAGTREIIFSKEL
                     YIEQDDFMENAPKNYFRLTPGQEVRLKSAYIVKCTGCKKNEKGEIVEVYCEYDPNTKS
                     GMPESNRKVKGTIHWVSVAHSLSAEVRLYDRLFRVENPSEEEGDFRELLNPDSLKILT
                     GCKVETFLQYAKPLDHFQFQRIGYFTVDMDATPENLIFNRTVSLKDSWAKEQKKK"
     gene            complement(14257..15036)
                     /gene="lpxA"
                     /locus_tag="PLR72_00475"
     CDS             complement(14257..15036)
                     /gene="lpxA"
                     /locus_tag="PLR72_00475"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOK35720.1"
                     /translation="MEQPLVYIHPEAKIDPSVTVGPFVFIDKNVEVGEGTTIGPHVTI
                     LEGSRIGKNCKIFPGAVIGAIPQDLKFRGEETLAIIGDNTTIREYVTVNRGTASKGKT
                     VIGNNCLIMAYCHIAHDCRIGNNVIMSNASQLAGEVVIDDYAILSGSVLVHQFSHIGS
                     HVIIQGGSKVNKDIPPYIKAGREPLSFEGINSIGLRRCGFTSEQICSIQEIYRYLYLS
                     GMNVSQAIERIEAELPATKEREDVLVFIRNSERGIVKAYFE"
     gene            complement(15054..16436)
                     /locus_tag="PLR72_00480"
     CDS             complement(15054..16436)
                     /locus_tag="PLR72_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOK35721.1"
                     /translation="MTKQKTIKETFTLHGKGLHTGLEISLTFIPAVENHGIKLKRIDL
                     PEQPIIEALAENVTHTIRGTVLQKGEVQVSTVEHALAALYALGIDNCLLEVNGPEFPI
                     LDGSAKHYVEKILEVGIEELNAKKDEFIVRKKIEYSLPDSKSKITLLPDDIFSIDVHI
                     GFDSPVLSNQFASLDSLDNFVTEIASARTFVFVHEIEPLLNMNLIKGGDLKNALVIYD
                     KEISQERMDFLTEKLNQKPIDASKLGYINNELNYPNEPARHKLLDVIGDLSLIGKPIK
                     GKVIANYPGHKINTEFAKLIRKEIKKQEVPVPIYDNSIPPVLDINAIRKLLPHRWPFL
                     LVDKIIEIHDKVIVGIKNVSGNETFFVGHFPNEPVMPGVLIVEAMAQTGGILVLNSIP
                     NPEKYSTYFLKIDNVKFRQKVVPGDTVIFRLEMIDEIRHGCTNMKGYAFVGGKIVAEA
                     QFMAQITKNN"
     gene            complement(16485..17543)
                     /gene="lpxD"
                     /locus_tag="PLR72_00485"
     CDS             complement(16485..17543)
                     /gene="lpxD"
                     /locus_tag="PLR72_00485"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOK35722.1"
                     /translation="MEFTAQQIADFLGGEIEGNPSVKVSGFSKIEEGKPGTLTFLSNP
                     KYNHYLYDCKASIVLINKDFKPEKEVTATLIRVDNAYESLAKLFSLINQNQIHKKGIS
                     SLAYIAPTAQIGENVYIAPFVFIGENVKIGNNSEIYAQCTIEENSTVGENVILYPGVK
                     IYHNCVIGNNCILHSGVVIGADGFGFAQSKEGIYEKIFQLGNVVLEDDIEVGANSTID
                     RATLGSTIIHKGVKIDNLVQIAHNVEIGENTVIVAQTGIAGSTKIGKNCVLAGQVGIV
                     GHLQIADNTQIGAQSGVTNSVKTPGQIIIGSPAIPIRNFRRSSILFKNLPEIQNLLIE
                     LKNKVEELEKNIQSGNPQ"
     assembly_gap    17606..17705
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17706..18745)
                     /locus_tag="PLR72_00490"
     CDS             complement(<17706..18745)
                     /locus_tag="PLR72_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOK35723.1"
                     /translation="MEQSNKLKIINDPVFGFINIPDDFLYEIIQHPYFQRLNRIKQLG
                     VSYYVYPGAQHTRMQHSLGCMYLVGEAIKELRQKGHFITTGEEQAVRACMLLHDIGHG
                     PFSHALEKSLVTDIDHEKISLLMMQRLNEEFEGKLTTAIEIFTNQYPKKFLHQLVSSQ
                     LDMDRMDYLSRDNFFCGVSEGIIGSARIINMLNIFNDDLVVESKGIYSIEKFLVARRL
                     MFWQVYLHKTCVAVERMLINILTRAKELALKGKELFSSPALHYFLYNYINEEKFSESA
                     EVLDNFANLDDSDLICAIKVWTNNEDKVLSYLCKSFINRKLFKVEMLEHGFHETKRQH
                     LLSQYQEHFKISA"
     gene            complement(18789..20234)
                     /locus_tag="PLR72_00495"
     CDS             complement(18789..20234)
                     /locus_tag="PLR72_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOK35724.1"
                     /translation="MINIEPSTVWYYFNEITKIPRPSKKEEKIRNYLLQIAKLYNLSA
                     KVDRAGNVLITKPATSGYENQQKIILQSHMDMVCEKNSEVDFDFETDAIQTYIEGDWI
                     KAKGTTLGADNGIGMAMMLAVLTSNDISHPEIECLFTVDEESGLTGASALEPDFLEGK
                     ILINLDSEDDGVIFIGCAGGIDTTANLSYIPRKTPLTNLSFSITVKGLKGGHSGEDID
                     KKHGNAIKILTRFLWNLSKEVVVHLHSLEGGGLSNAIPREATAIISIPFHQKENLSVL
                     FNQYIAEITSELQQSEPNLQFILSSEKMPYTVLDKRSSKILLNALYACPHGVKARSEE
                     IPDLVETSTNLASVKMIGNNQILITTSQRSSVESAKYDIAHQVESVFVLVGAKVHHSK
                     GYPGWKPNFNSEILKVAQQCYQEITGEEPKVRAVHAGLECGLFLEKYPQLDMISIGPQ
                     MYGVHSPEERLSISSTQKCWKWLVKILAHRL"
     gene            complement(20391..21257)
                     /locus_tag="PLR72_00500"
     CDS             complement(20391..21257)
                     /locus_tag="PLR72_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35725.1"
                     /translation="MSLNIWCHYLKLLFFIFLISVIVSCEKSDNEVTNSAYVTDVFEY
                     QYAPGQHANIVKPADAANFISLPSNVKSCLYLGGWGGYVIAGFDHNVINHEGYDFEVF
                     ALAGVSPEPAIVYVMEDKNGDGKPNDTWYELKGNQFENSKRNYWVRYYKAFSDSANIT
                     WKDSEGNTGELLAGFGTKYTASWWWKYYENDSITFYGTRLPDAYENNSTQWAVPVDRF
                     TWGYAENNFGIDYDQTVHSNQFDISNAVDSLGNSINLLHIRFIKIQTGVFQQAGWLNE
                     ISSEICGARELK"
     gene            complement(21287..22366)
                     /locus_tag="PLR72_00505"
     CDS             complement(21287..22366)
                     /locus_tag="PLR72_00505"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HOK35726.1"
                     /translation="MIKKGDQVRFLNAVGGGIVTRVDEKNKIIYVADNDGFEVPVLAN
                     ECVVIPAAINENTNFPVRNFKSESTKENTPSLSESKEPAFSFLEERVEIPEETPEGDM
                     LKLFLAFVPTNIKQLPNTSYECYLINDSNYYLFYNISLLKNTVALSLANGLIEPNMQE
                     LFAETTPEQLNDWLKINFQAIVYKKNKEYPIQQAIEVNIEVKIDKFYKLHCFTENDYF
                     DQPALLIDILSEKEKQQFLEISEKEIKKVISQKEVPVRLHKSKKPKKPQIIEVDLHIE
                     QLIDNPAGMSNFEMLQLQLNKCREVLEINKNNKGQKIVFIHGKGEGVLKKEIQKLLET
                     QYKKFYFQDASFQQYGFGATMITIR"
     gene            22430..23743
                     /locus_tag="PLR72_00510"
     CDS             22430..23743
                     /locus_tag="PLR72_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOK35727.1"
                     /translation="MIKLLSKNGNFLVLLLQIYNKFSVSPVKPANENIIPLNIEDFDY
                     NLPDERIAKYPLPQRDASKLLIYKNDAITESTFSHLVEFLPSNSLLVYNNTKVIEARL
                     IFYRETGARIEIFCLKPVVPSDYALSLGSFSECVWKCMVGNQRKWKGEVLTKEVNVDN
                     KNFLFQAELLEKKGNVSYIRFFWDAPDVSFAEILEKAGELPIPPYLHRKTEKIDLTAY
                     QTVYAKMKGSVASPTAGLHFTPEVFESLKTKNIETTELTLHVGAGTFQPVKTQNVLEH
                     EMHTEVFMVSKNTIEQLLDKLGNIIAVGTTSARTLESLYYIGLLLSQDSSLKEKPTSF
                     FLSQWLPYTMKNEISTKTALTLILEYLEKNKLSHLYAQTQIMIQPAYTFHLINGLITN
                     FHQPKSTLLLLISAFVGDDWRKIYHYALEHNFRFLSYGDSSLLLK"
     gene            23790..24578
                     /gene="pgeF"
                     /locus_tag="PLR72_00515"
     CDS             23790..24578
                     /gene="pgeF"
                     /locus_tag="PLR72_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOK35728.1"
                     /translation="MENLLTDSPLFDQFPDIQYFFTTRKGGFSYGNYATFNLSPYVGD
                     EPQCVAQNLNKLAETLSLPTHRIFFPHQNHGKFIHEIDETFLQLTPEEQKNRLAKVDG
                     LYTNLKNVCISVTTADCVPLLFYDPVKKAVAVAHAGWRGTVQQIAAKMVATLVRKYGC
                     SPKNIRVVMGPAISEQKYEVGKEILLTFIIAGFETSEIFIQQENKIFLNLWKANEMIL
                     LGSGVLKSNIEIKELCTFTNSSDFFSARREGTYCGRMLNGIILR"
     gene            24597..25256
                     /locus_tag="PLR72_00520"
     CDS             24597..25256
                     /locus_tag="PLR72_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOK35729.1"
                     /translation="MLKISISSLVHSICPDLVLGIIEGKVKNSPTSALLWQEVEKEME
                     EILSKYSLKQISKRPAILATRQAYKKFGKDPSRYRPSAEALCRRICKGNSIYQINTLV
                     DIINLVSIHSGFSIGGFDADKIQGNLLLDVGNENDEFEAIGRGILNVAGLPLFRDEVG
                     GIGTPTSDNERTKITMETNHLLMIINAYGGKTGLEKTIDYAVDLLKKYAEMTAYNIFL
                     S"
     gene            complement(25522..>26988)
                     /locus_tag="PLR72_00525"
     CDS             complement(25522..>26988)
                     /locus_tag="PLR72_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOK35730.1"
                     /translation="IYAQPKREMRAVWIATVANIDWPSSNRLTPREQRIEITNMLDKL
                     AQNNINAIILQVRPMADAFYPSSIDPWSHWLTGKQGFAPNPFYDPLQFIITEAHKRCM
                     EVHAWINPYRVTMNDNLSVLSPNHLFYQKPELFVRYGGKYYFNPGLDETRQILNNVIK
                     EIVEHYDIDGIHFDDYFYPYPVKGEEFPDEDTFRKNPRGFAPDQKADWRRNNVNMVIA
                     EVQQTIKNLKPWVEFGVSPFGVWRNNNVDPRGSATRAGIQNYDDLYADILKWLKEGNI
                     DYVVPQLYFEIGKKVADYEVLLKWWSENSYGKNLYPGLIPSSLGSNKNSAWSKGNEII
                     RQLRLNQQYPQVEGAVFFSAKTFLQNRLGLNDSLQQNFYKYPAICPVNKNIVGEPSAQ
                     PQNIRIVKDGDAAYLLWDEVEEEGGCQVAYYVVYAFKGKKVGDLNDPANIIARTTDNC
                     LDLRALNVHLKGHYTFVVTAINRFKQESIPTIAVTRKL"
BASE COUNT         8794 a         4722 c         4109 g         9263 t
ORIGIN      
        1 tacgtgatgt ttactttcag tatttttgtg ggtgtgtatt ttttgagcac cgctttccct
       61 ttgatccaag cgattttgtc cattttcgca atcgtatcgg cgaggaaggg atggaaaaga
      121 tttttgcata cagtgtgaag atacatggaa aagaagtaga gaagaaatcg aagttagttt
      181 tatctgatac tactgttcag caaaacaata ctacttttcc aacagatgcc aaattatgca
      241 agaaagtgat agacaaatgc aatacgatag cagaaaaaga agggatagaa caacgtcgtc
      301 gttacacgag agaaagtaag caattactgc gggatacgta caacgggact catcccaaac
      361 atgcaaggaa agcgaagaag gcaaagaaaa ggctcaagat gataaccaat aggtagatgc
      421 gggaattgga acgaaaaatg agcgaagagc agaggaggag atacgaaaag gagctggaat
      481 tatacaaacg ggcagtgaat caaaggaaag aggacaagga taagatttac agtcttcaca
      541 aaccatttac tcattgcatt tccaaaggga agccgcacaa gccttatgag tttggtaaca
      601 aggtaggatt gataacgaca ggaaagaagg gaaagaaaat cataaccgcc ataagggcat
      661 ttacagaaaa tccatttgat ggacatacga tagatccttt actggaacaa atgaaaggaa
      721 acgatttgaa gctaccgaag gagttggttt acgatagagg aggtcgaggg aagaaagaga
      781 tacggggagt aaaaatcata acaccaggta aacccagagc aagtgatacg gcttatcaaa
      841 aacaacagaa acgcaggaag ttcagagcaa gagcagacat tgaaccgatc atcggacatc
      901 tgaaaaaaga cttccgaatg gagcagaatt accttatggg agaagaaggc atacaaatta
      961 acgctttatt ggcagcttca gcttggaatt tgaagaaaat gatggaaaaa ctcaaagaag
     1021 agtttttttg tctcattttt cgattacttt ttcgccaaaa cttttataat cttgccactt
     1081 aaaaatagac tttttaagga atgactaatt aatttacatt taatttttta atttaaagga
     1141 cattatgata gcagttggtc ttgttggcaa ctataaagat aaaatatttt gtgaaatact
     1201 caacgaaaaa aactttttgg aattaaaaaa attcactatc ttaacgtgtg gataagtaac
     1261 ataggagaaa tatctgccta caaaaaagcg gaaagtgaga gattcgaact ctcggtacag
     1321 ttacccgtac aacggttttc gagaccgtcc cgatcgacca ctccggcaac tttccttttt
     1381 taacatggat ttcttctaaa actttatgaa tgaataaccc cgaggcaagc ctcgaggaat
     1441 tcttttgatt aaaaaattaa gttttaagaa ggaactcatc gtatgataac cagcgaacat
     1501 aacatagatc ctgacggtgt ggaagatcta tattttttgg aaacatccga aaaccaaatt
     1561 taaaagttcc tgacatattt aaacgataat caagtgcata atacaaggta gaaccgtctg
     1621 ctttgactaa tttgaattct tccacatcat acaatttata tttttgattt tgatccattt
     1681 ttgttactac caattcaatg ccaattcctt catcattgat actttttaca tcaattacga
     1741 tattcatttg ataaaaatcg ctgacttgag gattttgaaa taatttttca ggtatatcca
     1801 ttgatacaac ttcaatatta tcccagcctt gagcaatttt taatttccat gctgccagtt
     1861 cttttgcttt tgtatagtta tcttttttca atgttgctaa acggttactc aatttagagt
     1921 aaaaacgttc aatataatta tcgatcattc gcttggtagt aaagcgcggc gtgatgaggg
     1981 caatggagtt tttaatatat tgaatccatt ctggcgaaat tcccttagca tttttggcaa
     2041 aatacaatgg tataatttca ttttccagca tactataaat agtagcagcg tccagttgat
     2101 cttgatattg ttcattttca taagtacgtt tttcagtaat agcccaacca gcatctttta
     2161 cgtagccttc aatccaccag ccatccaaaa cagagaaatt aaggacacca ttcatttgtg
     2221 ccttttgtcc tgaagttcca gaagcttcta acggtctggt aggggtattt agccagatat
     2281 caactcctga aatcaagcgt tttgccatgg agatatcata attttcgaga aaaataattt
     2341 tccctaaaaa ttgaggcatt cttgaaattt caacaattct tttaattaag tcctttccac
     2401 cttgatccgc aggatgagct ttcccagcaa aaataaattg aacgggacgt tgagggtcat
     2461 taacaatttt ttctaaccgt cccaaatcca taaaaagcaa atgagctctt ttataagttg
     2521 caaatcgtcg tccaaagcca ataatcaagg catttggatt cagtgaattc aatattgtca
     2581 taattcgaga aggatcacct tgtgttttta accatttttc agggaattgg gtgcgaataa
     2641 actcaatcaa tttctttttc atctttaaat gagaatccca gatcacctga tcagggattt
     2701 catatattcc ctgccatatt tttacattcg attgatcatc ataaaactct tgtccaaaat
     2761 atttttcata taccgatttc cattctgaag tggtccacgt aggcaaatgt acaccgtttg
     2821 ttacataacc tacatgtaat tcttctggga aataccctgg ccagagagga ctaaacattt
     2881 cttgagaaac tttcccatgc aaccaactta caccatttac ttcctggcaa gtgtaacatg
     2941 caaacacact catactaaat ttatcattag gatcattagg tttttcacgt cctaaattca
     3001 taaaatcttg ccagttgatc tttaattttt gtgcatacat tttcatgtat ttggaaaaca
     3061 tttcttcatc aaaacgatcg tgacctgcag gtacaggtgt atgtacagta tacaaagaac
     3121 tacttctaac tacttcaaga gcttcgtaga aagaaagatt cttgtcattg atcaaatcaa
     3181 ttagacgttg aacattgatt aaagccgcat gtccttcgtt acaatgatag acgtcttttt
     3241 caatgcccaa tttttttaaa gcaagaattc ctccaatacc cagcatgatt tcctgtttta
     3301 acctattttc ccaatcacca ccataaagtt gataagtgat acttttatcc tcagggctat
     3361 taagttcatt atcagtatct aataaatata aatttattcg tcctatggct attttccata
     3421 aatttgcata tacagttcgt cctgggaaag gaacctcaat taacaaaggc tgattatttt
     3481 tatccttcaa ttgagttatt ggtaataaat taaagttttg tggatcataa atttcaattt
     3541 gttcaccatt aacggaaagc gattgcgtaa aataaccata acgatataat aaaccaatgg
     3601 ctgtcaaatc aactccatag tcacttgctt cttttaagta atctcctgct aatattccca
     3661 atcctcctga atagagtttt aaaacttcag ttaaaccgta ttccatactg aaataagcta
     3721 cgctgggctt atctggtgaa aatggtcgtc ccatataatt tttaaaatca ttataaagaa
     3781 catcaagctt gtttataaat tcagaatttt tttctgtttc aaccaattta tcataactta
     3841 agttatttag taattgaaca ggattaaatc ctgcctcttg ccaagcttca atgtctattt
     3901 cacgaaatag gtgacgtata tcgcttttcc atacccagca tatattgtaa gctaattctt
     3961 ctaatttttt taaaccatca ggaagataag atttaacatg aacatctttc catatcggtt
     4021 cgttaacaga attcatttta atttccataa tcaatttttc cttccaatta aatttttaaa
     4081 taacaaaaat tattattttg tcaaaaataa taaaagagta ataaactcct aaataaagta
     4141 taaaaaaaca aatatttatt acaaacgttt acattactaa attaataaaa agtaacgatt
     4201 aaaaatcatt tcaaaatatt tttataatca aattatcgat atatcttaag aaaaaattct
     4261 aattttatca tttattgtta gaagaataga ttatcttatt aacttttttg ggataagcga
     4321 tcatttctat gtgtaagagc tacttcataa gcttcatagt aatattgaat aaaatgttcc
     4381 cataaagctt tttcagcaat agtagcagct ctttttctaa taatttcaac ttgtgcttct
     4441 gtataacctg aaaaatgaga tacttgttct gcaatattaa ggcttacttc atactgatta
     4501 taatcggaac gatgaacaat tcctaccccg ttttcaattc cctggggttg ttgagcaacc
     4561 catcgtccaa aacctgaaac atcagtagta attgtaggaa cagaaaaagc aacactttcc
     4621 agaggagtat aaccccaggg ttcataatat gatggaaaaa ctgtaaggtc catccctatc
     4681 agtaattcat aataggattt attaaaaata ccgtcgaagc cattcaaata agaaggcaca
     4741 aaaataactt ttattttttg agaagcctga tttgtaaaat gaaaccaccg gatggcggac
     4801 attatattat cattttgata atcatataat tcatgagtcg taaatcggtt ccaagaagta
     4861 attttagaaa ttgaatgttt taagcatgca agtaaatctg cacgaggacc tgaaatatga
     4921 gcaggaatca taaggaaagc tacaatctct ttatttaaat ttgaatgttc taacaaacgt
     4981 cttaatgatt caataaaaac atcaattcct ttgtttttaa attcataacg tcctgcagta
     5041 caaacaaata aaacatcttt ctttaactta tagcctaata aagtttcgga tacttttaat
     5101 aaaatattgc gagcttcttg tctttttaat tgaaactttt cttttgaagg aacaaaatca
     5161 tcttcaaagc cgttaggggt gactacatca ggtttttttt cgagcaactg agcacattcc
     5221 tttgcagtaa tttcacttac tgtggtaaaa cagtctgcca gatgagcagc agttttttct
     5281 gctgaatgtt ttgagaccac atttaattca taagacattt gatcaccatt atactcattc
     5341 aaataatcgt acaaaggttt attgtttgtt gctatggaac gtccaacggt tgtggcatga
     5401 gtagtaaaaa cagtagcaac ctgtggcata tgctgtttca aataaaaaat cccaaaagca
     5461 gtctgccatt cgtgaaaatg acctactacc ttctgttgat tttctaaatg aaagaaatga
     5521 taataacttt caataatcat tcctgcagca tatccaaaca ttgaacattc aaaataatcc
     5581 ccataagctg gaagagaatt tactccggta ttcagccaga cttgtgcata aatttcattc
     5641 ctttggggca tcaatggcat aaaatcaacc aagatagtta atggttttcc aggaacattc
     5701 cagcgtccaa ttctaatgtc caaatggtac tgtttatgat gaaaaaattt ccaatcagta
     5761 tataaatttt gatcttcctc aaaaagtgga ttttcttttt gaagccacaa gtcaggacca
     5821 ataaatatta atttctcatt gtattccttt tgtaaggtag cagcatgagt tgataatact
     5881 gtataaattc ctccaacttt gttacagact tcccaactta cttcaaaaac atattcaggt
     5941 atcaacattt gattagaaca tttttattta aaaaaattaa gcacaaattt aatatatcag
     6001 tacgtgaaaa aatgggaaat aattgaaaaa aataacaaaa attaggcatt tacttcaaga
     6061 aaatataaat catcagaaat tttttacttc aatttttttc atatttttca caaaaaaatt
     6121 tctcctttaa aaagaggaga aattttaaac gtaaatatca agtgctattt taataattcc
     6181 tcttattatt ttttttgctg actacaacgt tgatttaatt gtttaataac ttctgaagta
     6241 atatcatatc catcaactgc atacaaaata ttatcatttg aagtattact caaaataata
     6301 tgatattttt tgtctttatt aaattctttt aaaaaagcat tgatgctatc acgtaattgt
     6361 tcactcattt tttgttgttc ttccaacaat ttttgtgata attgggcgtc taatttttgc
     6421 aaatcctgtt gtttttttat taaccttgat tgttcttgtt cggcacgttc gcgacttaaa
     6481 aaagcattat tttccaattt tttttgaaat tcattcattt cattctgaag ctcacgagct
     6541 ttcatattta tcgtcaatcg agaatcttct tgctttttaa gtaaagtttc attagcatct
     6601 tttgcaaaat gataatgaag taacaaagaa tctatattta tataagcaat gggtaattgg
     6661 tttgtatgaa gagcttcagt agttgttatt atttttcttt cttctttatt tgtctttaaa
     6721 ttaaaaaata aaataaataa cacaattacc gcaatgcata aaatagcatt taaaacgagt
     6781 aaaaatttat tcatatattt ctaaattcta ttgttattat tattaatgaa tttgttatta
     6841 gctttttata aattcttgaa tctcaattat tctaaattga tttttgttta cctactttta
     6901 aaaaacaaca catcctattt tttccttact aaaagttgat ctattttcaa ccacattttc
     6961 aattgtagaa aaaatcattg taaaaaatga tataaattac tttcaggaat aatttctttt
     7021 attttctaaa tcattttata taattttttc tcactgagaa gaaataattt ttgaactatc
     7081 aagtacattc ctcatttttc ttatgcaaag ataaattatt tagaatataa tgttactttt
     7141 tgctttgttt ttttagcaaa atgaatattt tgttttattt tttaacacaa gttttaatag
     7201 ctcaaatcat aatcttagga taaattgttc ccgtgtttaa gagcatattc gcttgaagaa
     7261 acaccgtaaa actctttaaa agagtttgaa aaatgtgata aatttgaaaa tccaacagaa
     7321 taggcaactt cggtttaaaa atggggcgat tatcttgaca aagaattaac atttcgaata
     7381 gaagcaatca tagcagattc cgcaaatgac tgggaatatg aaagtggaac aaaagtagtt
     7441 atactatctt tatccatatt gttgatttgc agggaattat tactacgatt cgatgtgata
     7501 ggcagcattt tcaattccct ctttgtgatt tggatatttt gggtaaaaac aaacgaaaca
     7561 ttcaattaat tgaagattac caagtatagt ttgggaataa gccgttttaa aaaaatgctt
     7621 tcccctaata attcaatttt ttataaaaat ccttttacaa tcaaatactc aaaatattat
     7681 acgttataaa tcgctttttg cagggggaaa atagattttc ccaaaaaaat catttttctc
     7741 ctattttatt tctcgttttt cacaaaaaac cataaaagtc ctgaaatggt aagtgtgatg
     7801 gtacaaatag caaaaattat ggatttatta gcagccatat ttataacctc acccattttg
     7861 aaactcgcta ccaactgagg caacactact gatagattaa atatttccat atataaaccc
     7921 atttttgatt gatcaacttt ctcacttata attgcaaatg gaagtgaaac tactgatccc
     7981 cagccaatgc caacaattgc tataaaaaga taatttttat tccatcattc cacaattttt
     8041 ttgtttcctc gcaaactctt atctcgaacc agcaccgtat gtctcgcaat tagcaaactc
     8101 ctgaccatca acaaaattaa tcgcaccata ttcttcaaac cttgctactc gttctctcac
     8161 aaattcgaaa ctcgtaactc attaactttt gagtcatcaa acaaggtcaa ttttttaact
     8221 ttatcttgct taaaacctta ataacaaatg ataaacgaat cataccaacc ttattagctt
     8281 attaaaaaca gaaaatgata taaaaagaat tgttttctaa gtatttaaaa atttttggaa
     8341 cattcagcct aattttgtaa aaaatatttg attacttgat aaatatttat ttaatttgtg
     8401 ccaataaaag gacaaataaa ctttttcaga agatgaaaga tattttaaat tttttgggtg
     8461 ctattatcgt tttaataggt gtttgtatat taggagtata tcattttgct ggtattaact
     8521 caaatacttt gttagcaatt gctggattat taatgataat aggttttttt acttttcttt
     8581 tcatgaataa aaaatctctt aaaaaagatt tttaagtacg ttggtttttt atttttaatt
     8641 ttttttataa ccaaattttg agaaataaag atagggacat ttcatgatag aatgtttcta
     8701 tctttattat taaaacaaag ataattgctg tgaggtttca tcatgatttt ttttatcatt
     8761 taccaattca tctggtaaaa tagaatcttt ttcagaaaaa atcattgttg attcttcttt
     8821 ttctatttct gaagcctctt ctaatgatgt gaaatttttt tctaaaattt ctgactcttc
     8881 gtttctatca tttccgtttt cttctatttc aatataatcg cttacctgtt ctgtatcaat
     8941 ttcttgtttt gtatgcaagg gttctaactc attgatgatt tcaatttcat gggtagtaat
     9001 acgtttccct tttgccttgt agcttttcgc cgaaataaat tcactgactt cgatcaccaa
     9061 aggttttcga taagcatctt tgccaccaaa aattacctca aagcgtggaa aatccttatc
     9121 agaaagaaga attaaacgcg actcaggatt ttcgccaata aagcttactt tcttttgcga
     9181 tggtgataat tggaaacgtt tcaaataata atggttctgg tcagcatcaa aaatagcagc
     9241 agaccagatt ttatcttcct cgaacttttc aagtcttaaa atattgtttt cataatgatt
     9301 attgacgtca aaatcagtag tataatattc cccttgttga gtaataacta agataagatc
     9361 atcattttga aattctccca gatattcgcc tcgtttgtca taattcagac gcaacacatc
     9421 atgatcaaac caaacttctc tgccacctaa ggtagaacct cctttttgtt tcaaggtgat
     9481 tttatgaatt tcgtttttgg ttaaaatgct ccctcttgtc tgacgacctt taatagcaat
     9541 ttcactaaaa tccttttcaa aaaccaattt ctgtaaacgc ggttttggtt ttaaagtgac
     9601 gcgtattatt tctgcttcag cattgggatt ggctgaaaaa taaacaattt ttgaacctgg
     9661 cttcccctga gtaagatcat attctttatc gcgagtgatg ctattcactg caaaacgttt
     9721 gatataataa ggtcctgttt tcccgtcacg atatactaca ttataaaccg tgcgtttatc
     9781 attcttttta aagacattga tatagagaat atttttccca acaaacattt tttcagctac
     9841 tttcaccact ttatatttac catctttaaa aaagacaatg atgtcatcta tttcggaaca
     9901 attgcataca aattcatctt tcttcaacga agttccaata aaaccttctt ctcgattgac
     9961 ataaagtttt tcgttggctt cgataacttt tttagcttca atggtttcaa aattgcgaat
    10021 ttctgttcgg cgtggatatt ttttcccata ttttgccttt aaactttcgt accaggcaat
    10081 agtataatct atcaaatgag aaagttgata atctgtttcc tcaatttttt tctgaatttc
    10141 tattaaagta ttattggctt tgtcggcatc aaatttggta attcgtacca tttttatttc
    10201 taatagtttc aaaatatctt ctcgggtaac ttcacgaatc agattagctt taaacggttc
    10261 gagttgttta tcaatgtact gaatagcaac ttcatgagag gtactgtctt caaaaccttt
    10321 ttccttatag atacgatttt caataaaaat tttttccagt gaaatgaaaa agaattgttc
    10381 ttctagttct tttttacgaa ttttcaattc tgcttccaaa agattttttg tatcctcgca
    10441 actcacgcaa agcatttcgc tgacgctcat gaatcgaggc tttttatcgt gaatgacgca
    10501 acaatttggc gaaatactta tttcacaatc ggtaaaggca tacagagcat caatggtttt
    10561 gtcagacgaa gtacctggct ggagataaac caaaatttca acattagaag aagtattgtc
    10621 atctattttt ctgattttaa tttttccttt gtcatttgct ttcagaatag aatcaataag
    10681 tttggaagtg tttgtgctat aaggaatttc agtgatagcc aatgttttgt tatctatttt
    10741 attaattttg gctctgattt taacaacacc gcccctttct ccatcattat aacgacttat
    10801 atctatcaga ccacctgttt gaaaatcagg ataaagaaca aattcttctc cttttaaata
    10861 agcaatacaa gcatcaagaa gttcgttgaa attatgaggt aaaattttag agtttaatcc
    10921 aacagcaatt ccttcacatc ctaaagctaa aagtaatgga aatttaacag gtaaagttac
    10981 tggctctttc tttcgtccgt catacgaagg tttccattca gtgatttttg gattaaagac
    11041 ggtttcgaga gcaaattttg agagacgtgc ttcgatatat cgtggagcgg ctgctgcatc
    11101 acctgttaaa atgtttcccc agtttccctg acagtcaatc aacaaatctt tttgtcctaa
    11161 ttgaaccagc gcttccgtaa tggaagcatc accgtgcgga tggtattgca tggtttgacc
    11221 aacaatatta gcaactttat tgtagcgtcc atcgtccatt tccttcatag aatgaagaat
    11281 acgtcgctga acaggcttca aaccatcatg aatatcaggc acagctcgct ccagaatgac
    11341 ataagaagca taatccagaa accagttttg aaacattcct gaaagatgat gtttcacact
    11401 ttcatctgtt attttgggaa cttgataagt tgaatgccct gtatcaaaag attcggaatt
    11461 taattcttcc tcgtcagttg ataatatatt ttcattttct tcctcaaata tttcttcgtc
    11521 gctcatcttt atttataata tcttgtctaa taaattattc tcttgactta tttaataatt
    11581 cccgaatcac aaaggaagtc aaataacatc caaataccgc aggcatatat gaaatactcc
    11641 caatagttga ttttttgttt gcttcatttg caacaggaat aatggatttt tttatcactg
    11701 gttcggttga ataaactacc ggaacccctt ttttaatacc tatttttgcc aattgttgac
    11761 gcacagctct tgccagactg cacatggtgg tttccgaaat atcttttatc atcacttttg
    11821 atggatttaa ttttcctcct gcacccatag aagaaatgat atgaatgttt cgttccacac
    11881 atgattttat caaaaaaact ttggacatta acgtgtcaat ggcatctatc acaaaatcaa
    11941 aatgattttc ttccaacatt gatatcgtat tggaaaagtc cagccattta tggtgaataa
    12001 tcaacttcaa ctcaggatta atttgcgaga gtcgttctgc catgacttct acttttgggc
    12061 gataaagagt agatgttaaa gcaataattt ggcgattgat attactcgca tttactatgt
    12121 caccatcaat gagggttatt tttccaatac ctgcacgaca aatcatttct gttgccatcc
    12181 ctcccacccc tcctattcct acaatcagga ctgaagaatc ttgtaaaatt ttcaatcctt
    12241 tttctccaat taataattcg gtgcgatctg tccacgtttc catacaatga ttgcttaact
    12301 tttctttata caaagttatc agtttaaaac tacaattcca tattttttaa aaagctttat
    12361 cattttttat tttatatcga ggaattcata aataaaaaac tgtcacaact acatgcaaca
    12421 gtttttttat cacaatactt gtaaaagaaa attcattttt tcttttgttc ttttgcccaa
    12481 ctatctttga gtgaaacggt acgattaaag attaaatttt caggtgtagc atccatatct
    12541 acagtaaaat aaccaattcg ttgaaattga aaatggtcca aaggtttagc atactgcaaa
    12601 aaagtttcca ctttacagcc ggtcagaatt tttaaagaat ctggattgag caattcacgg
    12661 aaatcacctt cttcttccga aggattttca actcggaaca aacgatcata taaacgaact
    12721 tctgcggata atgaatgagc aactgaaacc caatgaatag tacctttaac tttgcgattg
    12781 ctctcaggca ttccactttt tgtgtttgga tcatattcgc aataaacttc aacaatttca
    12841 cctttctcgt tttttttgca gcccgtacat ttaacaatat aggcactttt taatctgact
    12901 tcctgaccgg gagttaggcg aaaataattt ttcggtgcat tctccataaa atcatcttgc
    12961 tcgatataca attcttttga gaaaataatt tctcgtgtcc ctgccgaagg atcttcggga
    13021 ttattgggcg tttgtagtat ttctatttca ttatcaggat aatttgtaat aatcaatttc
    13081 actggataaa gtactgcgct aactcgtgtg gcaattttat tgagattttc acgtgctgaa
    13141 tactccagca aaccaatatc gtttaatgca tcgaatttag tataaccaat tttgtttata
    13201 aagtttcgaa tagcttcggg agtgtatcct cttcttctaa gtccacaaag agttggcatg
    13261 cgaggatcgt cccaaccgtt caccagacct tcctgcacca gttggagtaa ttttctttta
    13321 cttaaaatag tataggttag atttaaacga ttaaattcaa tttgtcgtgg tcgataatct
    13381 gaatcttgat actgatcaat gaaccaatca taaagtggac gatgaacttc aaattcaagt
    13441 gtgcatagag aatgtgtaac tccttcaaaa taatcacatt gcccgtgagc aaaatcatac
    13501 atcggataca ctttccattt ccaacctgtg cgatgatgag gatgcgaggt aataatacga
    13561 tacataattg gatcgcgcat gtgcatattg ggcgaagaca tatcaatttt tgctcgcaat
    13621 accattgctc cttcaggaat ttcacctgat gacattttat aaaacaattc aagattttct
    13681 tcgacgggac gattacggta tgggctttcg ataccaggaa tagtaggcgt tcctttctgt
    13741 tttgctatga tttcagcgga ttgttcatca acataagcca attctttttt gataaaaaaa
    13801 acagctaaat cccaaagctt ttgaaagtaa tcagaagcat aatatacatt tttccactga
    13861 aaaccaagcc attgaatatc ttccataatg gaatcaacgt attcaacgtc ttcttttaat
    13921 ggattggtat cgtcaaaacg taaattacaa atgccaccat atttttcggc aatgccaaaa
    13981 tcaaggcaaa tagctttagc atgaccaata tgtaaataac cattaggttc aggtggaaaa
    14041 cgtgtctgaa ttctacctcc atttttcccc tcctttaaat cttgttcaac gattgcctcg
    14101 ataaaattca agtttcgttt ggaaggttct tctttgtata tcatatattt attactcaaa
    14161 ttatttttat atcaacctct tttaacgata attgatgtag atttgaaaag agaataaaaa
    14221 agattttttg atagtttgat agaaagagta aaatatttat tcaaagtaag ccttgacaat
    14281 acctctttcg gaattacgga taaatactaa aacatcttcc cgttcttttg tggcaggcaa
    14341 ttcagcttcg atccgttcaa ttgcttgcga aacattcatc cctgataaat ataaatagcg
    14401 ataaatttcc tgaatactac aaatttgttc acttgtaaaa ccacatcgac gtaaaccaat
    14461 agaattaata ccttcgaatg aaagtggttc acgacctgct tttatataag gaggaatatc
    14521 tttatttact tttgaaccgc cttgaatgat cacatgcgaa ccaatatggc taaactgatg
    14581 tactaacaca ctcccactta aaattgcata atcatctatt actacctcac ctgccagttg
    14641 agaagcatta gacataataa cattgttccc gattcgacaa tcatgagcaa tatgacagta
    14701 tgccattatc aaacaattgt tgccaattac agtttttcct tttgaggctg taccgcgatt
    14761 tacggtaaca tattcgcgaa tggtagtgtt atcgccaata atggcaagag tttcttcccc
    14821 tctaaatttt aaatcttgcg gaatagctcc aattactgcg ccaggaaaaa ttttgcaatt
    14881 tttcccgatt ctacttcctt caagtatagt tacatgagga ccaatggtag tcccttctcc
    14941 aacctctacg tttttatcaa taaaaacaaa aggtcctact gtgactgatg gatctatttt
    15001 agcttctgga tgaatataga caagcggttg ttccattttt tctatatcaa cttttaatta
    15061 ttttttgtaa tctgagccat aaattgagct tctgctacaa ttttcccgcc aacaaaagca
    15121 tatcctttca tattagtaca gccatgtctg atttcgtcta tcatttcaag acgaaagata
    15181 acagtatcgc caggaactac tttctgacgg aattttacat tatctatttt taaaaaatag
    15241 gtagaatatt tttcaggatt aggaatagaa ttcagaacta aaattccacc tgtttgagcc
    15301 attgcttcta caattaaaac tccaggcatc acgggctcgt tggggaaatg acctacaaag
    15361 aaagtttcat ttcctgaaac attttttatt cctacaataa ctttatcgtg tatctcaata
    15421 attttatcaa ctaaaagaaa aggccatcgg tgaggtaaaa gttttctgat ggcatttata
    15481 tccaatacag gaggaatgct gttatcataa ataggaacag gaacttcttg cttttttatt
    15541 tctttacgaa ttaatttggc aaattctgta ttgattttat gtcctggata attggcaatt
    15601 actttacctt taatgggttt accaatcaat gataaatcac caatgacatc aagaagttta
    15661 tgtcttgctg gttcatttgg ataattaagt tcattattaa tataacctaa tttggatgca
    15721 tcaataggtt tttgatttaa tttctcagta agaaaatcca ttctttcctg cgagatttct
    15781 ttgtcataaa ttaccagtgc attttttaaa tctcctcctt taataaggtt catgttcaat
    15841 aaaggttcaa tttcatgaac aaaaacgaaa gtacgagcag aggcaatttc agttacaaaa
    15901 ttatccaatg aatcgagtga tgcaaattga ttagagagta ctggtgaatc aaatccgata
    15961 tgaacatcaa tgctaaatat atcatcgggc aataaagtga tttttgattt agaatctggc
    16021 aaagaatatt caattttttt tcgaacaata aattcatctt ttttagcatt cagttcttct
    16081 attccaacct ccaaaatttt ttcaacataa tgtttggcac taccatctaa aataggaaat
    16141 tctggtccgt taacctccag caaacaattg tcgattccta aagcatacaa agctgccagc
    16201 gcatgttcta ctgtgcttac ttgaacttct cctttttgca gtacagtacc acgaattgta
    16261 tgggttacat tttccgctaa agcttcaatg ataggttgtt caggaagatc tattcgtttc
    16321 agtttaattc catgattttc aactgctggt ataaatgtta aagagatttc taaacctgta
    16381 tgtaatcctt tcccgtgaag agtgaatgtt tcctttattg ttttttgttt tgtcatcatt
    16441 ttacttgtta gaataaaaat cttttataca aggcagtact ataattattg aggattacct
    16501 gattgaatat ttttttcaag ttcttccact ttgtttttca attctataag aagattttgt
    16561 atttccggta aatttttaaa aagaatagaa gaccttctaa aattacgaat gggtatagca
    16621 ggagaaccaa taataatctg acctggtgtt ttcactgaat ttgtcacacc tgattgtgca
    16681 cctatttgtg tattgtctgc aatttgtaaa tgtcctacaa ttcctacttg cccggctaaa
    16741 acacaatttt tacctatttt tgttgaacct gcaatccctg tttgtgccac gataacagta
    16801 ttttctccaa tttcgacatt atgagcaatc tgcaccaaat tgtctatttt tacccctttg
    16861 tgaataatag tactacccaa tgtagcccta tcgatcgtag aatttgctcc cacttcaata
    16921 tcatcttcca acacaacgtt gcctaattga aaaatctttt cgtagattcc ttctttggat
    16981 tgtgcaaaac caaaaccatc ggctccaatc acaacaccac tatgcaaaat acaattgtta
    17041 ccaataacac aattatgata aattttcact cctggataaa gaattacatt ttcgccaact
    17101 gtagaatttt cttcgatagt acattgagca tatatttcag aattatttcc aattttaaca
    17161 ttttctccaa taaacacaaa aggagcaata taaacatttt caccaatttg tgccgtagga
    17221 gcaatgtatg ccagtgacga aatgcctttt ttgtggattt gattttgatt aatgagagaa
    17281 aaaagtttag ctaatgattc ataagcatta tccacacgaa ttaaagtagc agtaacttct
    17341 ttttcaggct taaaatcttt gttgattaaa actatactgg ctttacaatc ataaagataa
    17401 tgattgtatt tgggattgga cagaaaagta agcgtgcctg gctttccttc ttcaatttta
    17461 gaaaaaccac ttacttttac agaaggatta ccttcaattt ctcctcctaa aaaatctgct
    17521 atttgttgag cggtaaactc catagttaaa aattttggct acaaaagtac taaactattt
    17581 ttaatttttc acttttagaa ataaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17701 nnnnngcaga aattttaaaa tgttcttgat attgacttaa caaatgttgt cgtttagttt
    17761 catggaaacc atgttctaac atttcaactt taaataattt tctattaata aacgatttac
    17821 ataaatatga taaaacttta tcttcgttat ttgtccaaac tttaatagca caaatcaagt
    17881 ctgagtcgtc aagatttgca aaattatcga gtacctcagc cgattcacta aatttttctt
    17941 catttatata attgtataaa aaataatgta gagctggaga agaaaaaagt tctttacctt
    18001 tcaatgccaa ctctttggct cttgttagta tattaattag cattctttca acagctacac
    18061 atgttttatg caaataaact tgccaaaaca tcaaacgtcg tgccacaagg aatttttcaa
    18121 tagaataaat gccttttgac tcaactacca gatcgtcatt aaatatattt agcatattaa
    18181 taatgcgagc agaaccaata attccttcac ttacaccaca aaaaaaattg tcacggctca
    18241 aataatccat tctgtccatg tccagttggc ttgaaaccaa ttgatgtaaa aatttttttg
    18301 gatattgatt ggtaaaaatt tcaatcgcgg tggtcaattt cccttcaaac tcttcattga
    18361 gacgctgcat catcaaaaga gaaatttttt cgtggtcaat gtccgtgacc aaactttttt
    18421 ccagtgcatg ggaaaaaggt ccatgtccta tatcgtgcaa cagcatacaa gcccgaacag
    18481 cttgttcttc gcctgtagta atgaagtgac ctttttgtct caactcttta atggcttcac
    18541 caaccagata catgcaacca agtgaatgtt gcatgcgggt atgttgggcg ccaggataaa
    18601 cataataaga aacgcccaat tgtttaatac gatttaatcg ctgaaaataa ggatgttgaa
    18661 tgatttcata taaaaaatca tcaggaatat tgataaaacc aaaaacagga tcgttaatta
    18721 tttttaattt attcgattgt tccatttttt tttatctatg ctgattaaat gaaatattga
    18781 gagaacgttt ataacctatg tgccagtatt ttcaccagcc atttccagca tttttgagtt
    18841 gaagatatac tcaatctttc ttccggagaa tgaaccccat acatttgtgg tccaatagaa
    18901 atcatatcca actgaggata tttttcaaga aaaagtccac attccagacc tgcatgtact
    18961 gctcgaactt taggctcttc tcctgtaatt tcctgataac attgttgagc tactttaaga
    19021 atttcagaat taaaatttgg cttccatcct ggataacctt tactatgatg tacttttgct
    19081 ccaaccaaaa caaaaactga ttctacctga tgagctatat catatttggc cgattcaaca
    19141 gaacttcgtt gactggtagt aatcaaaatt tggttatttc ctatcatttt tactgaagct
    19201 aaattagtag aagtttcaac taaatcggga atttcttcac tcctggcttt tactccgtga
    19261 gggcaagcat aaagagcatt gagaagtatt tttgagcttc ttttatccaa aacggtgtat
    19321 ggcatttttt ctgaagaaag tataaattgc aaattaggct cgctttgttg taattcagat
    19381 gttatttctg caatatattg attgaataaa acactcaaat tttctttttg gtgaaaagga
    19441 atagaaatta ttgcagtagc ttcacgtgga atggcattgg ataaaccacc accttccaga
    19501 ctatgaagat gtactactac ttctttgctt aaattccaca ggaatcgagt caaaatttta
    19561 atagcattgc catgtttttt gtcaatatct tcccctgaat gtcctccttt tagaccttta
    19621 actgttatgg aaaaagataa attagttagc ggagtttttc gaggtatata tgaaagatta
    19681 gctgtggtgt caattccacc ggcgcaacct ataaaaataa caccatcatc ctctgaatcc
    19741 aaattaatta aaatttttcc ttccaaaaaa tcaggttcta aagctgaagc accagttaaa
    19801 ccactttctt catcaacagt aaacaagcat tctatttccg gatgggaaat atcattggaa
    19861 gtcaagacag ccagcatcat agccatacca attccattat ccgcacctaa agttgttccc
    19921 tttgctttaa tccagtcacc ttctatgtaa gtttgaatag catcagtttc aaaatcgaaa
    19981 tccacttcac tatttttttc acataccatg tccatatgtg attgtaaaat aattttttgc
    20041 tgattttcgt aacctgaagt ggctggttta gtaatcaaaa catttcctgc cctatcaact
    20101 tttgctgaaa gattatatag ctttgcaatt tgaagaagat aatttctgat tttttcttcc
    20161 ttttttgaag gacgaggaat tttagttatt tcgttaaaat aataccagac tgttgaaggt
    20221 tcgatattga tcataatgta gttttgagtt ttgaatttcg agttacaaat ttcataggaa
    20281 atgaaaattt aacaaagata attaaaattt catagataga agctaataat ttacaaagaa
    20341 atataagatt ttacgctatt ttttgaaatg gaatttatcg tatatttcta ttattttaac
    20401 tctcgagctc cgcaaatttc tgacgatatt tcattgagcc aaccagcttg ctgaaaaact
    20461 ccagtttgaa tttttataaa acgaatatgt agtaaattaa ttgagttacc caaactatct
    20521 acagcgtttg aaatatcaaa ttgattggaa tgaactgttt gatcgtagtc aataccaaaa
    20581 ttgttttcgg cataccccca tgtaaaacga tcaacgggaa cagcccattg agtactatta
    20641 ttctcataag catcaggtaa acgtgttccg taaaaagtga tgctatcatt ttcataatat
    20701 ttccaccacc atgaagcagt atattttgta ccaaaacctg caagtaattc tcccgtattt
    20761 ccttcgctgt ctttccaagt tatgttggct gaatctgaaa aagctttata atatcgcacc
    20821 caataatttc tttttgagtt ttcaaattga ttccctttta attcgtacca ggtatcattg
    20881 ggttttccat cgccgttttt atcttccatt acataaacaa tagcaggttc aggcgaaaca
    20941 cctgccaaag caaatacttc aaaatcatat ccttcatgat tgatcacatt atgatcaaag
    21001 ccagctatca catatccgcc ccaaccaccc agatacagac aactttttac gttagaaggt
    21061 aagctaataa agtttgctgc atctgcaggt ttaactatgt tggcatgttg accaggtgca
    21121 tattgatatt caaaaacatc tgtaacataa gcagaattgg ttacttcatt gtctgatttt
    21181 tcacaagaaa caattactga gattagaaaa ataaaaaaca atagttttaa ataatgacac
    21241 caaatattaa gagacatttt tctgtaaatt aaaaaataaa aaaaaattat cgaatcgtta
    21301 tcattgtcgc tccaaaacca tattgctgaa acgacgcgtc ctgaaagtaa aattttttgt
    21361 attgtgtttc cagaagtttc tgaatttctt tttttaatac gccttcacct tttccatgaa
    21421 tgaaaacgat tttttgacct ttgttatttt tgtttatttc cagtacttcc ctgcatttgt
    21481 tcaattgaag ttgaagcatt tcaaaattac tcattcctgc tggattatct attaattgtt
    21541 caatatgtaa atcgacttca atgatttgtg gttttttggg ttttttagat ttgtgtaagc
    21601 gaacaggaac ttctttttgt gaaattacct tttttatttc tttttcagag atttcaagaa
    21661 attgttgctt ctctttttct gataaaatat ctatcaataa agctggttga tcaaaataat
    21721 cattttcggt aaagcagtgt aatttgtaaa atttatcgat tttcacttca atatttacct
    21781 caattgcctg ttgaatggga tattctttat tctttttata aacgattgct tggaaattaa
    21841 tttttagcca atcatttaat tgctctggag tggtttcggc aaaaagttcc tgcatgttag
    21901 gttcaatcaa tccattcgcc aaactcaaag caacagtatt ttttaataaa ctgatgttat
    21961 aaaacaggta ataattacta tcattgatta aataacattc ataactggtg tttggcaact
    22021 gtttgatgtt ggttggtaca aaagccaaaa acaatttgag catatcgcct tcaggtgttt
    22081 cttcaggaat ttcaactctt tcttcaagaa atgaaaaagc tggttcttta ctttcagaaa
    22141 gagagggtgt attttctttg gtactttcag acttaaaatt ccgaacagga aaatttgtat
    22201 tttcatttat tgcagcagga attacaacac attcatttgc caatacaggc acctcaaaac
    22261 catcattgtc tgccacgtaa atgattttgt tcttttcatc aacacgcgtc actatgccac
    22321 caccaacagc atttaaaaaa cgtacctgat ctcctttttt tatcatgata agataaattt
    22381 tagaatagta taaaaattgt catttcttac ttttaaagtg tgaaaagtaa tgataaaact
    22441 actatcaaaa aatggcaatt ttttagtact tttgctgcaa atttacaaca aattttctgt
    22501 aagcccagtg aaacctgcca acgaaaatat cattccactg aatattgagg attttgacta
    22561 caacctgccc gacgaacgta ttgcaaaata tcctttgcct caacgtgatg cttcaaaatt
    22621 attgatatat aaaaatgatg ccatcactga aagcactttt tcgcatctcg ttgagttttt
    22681 accatcgaac agcttgttgg tttataataa tacaaaggta atagaagctc gtcttatctt
    22741 ttatcgcgaa acaggtgcac gtatagaaat tttttgtttg aagcctgttg ttccttctga
    22801 ttatgcctta tcgcttggtt ctttttcaga atgtgtatgg aaatgtatgg ttggcaatca
    22861 gagaaaatgg aaaggagaag tgcttacaaa ggaagtaaac gttgacaaca aaaattttct
    22921 ttttcaagct gagttgttgg aaaaaaaagg aaatgttagt tatatccgct ttttctggga
    22981 tgctcccgac gtttcttttg ctgaaatttt ggaaaaagca ggagaattgc ctattcctcc
    23041 ttatcttcat cggaaaacgg aaaaaattga ccttaccgcc tatcaaactg tttatgctaa
    23101 aatgaaaggt tctgttgcat ctcctactgc tggtttacat tttacacctg aagtttttga
    23161 aagcttgaag acaaaaaata ttgagacaac cgaattgact ctgcacgttg gtgctggtac
    23221 ttttcagcca gtgaaaacac agaatgtttt agagcatgag atgcataccg aagtgttcat
    23281 ggtttcgaaa aatactattg aacaattact tgataagttg ggaaatatta ttgctgttgg
    23341 aactacttca gcacgaacgt tggaaagtct ttattatata gggcttttat taagtcagga
    23401 ttcgtcgctg aaagaaaagc cgacttcatt ttttctttct caatggttac catatacgat
    23461 gaaaaatgaa atatcaacaa aaacagcatt aactcttatt ctggaatatt tggaaaaaaa
    23521 taaattgagc catctttatg ctcaaacaca aatcatgatc cagccggctt atacatttca
    23581 tctgataaat ggattgatta ctaattttca tcagccgaaa agtactttat tactacttat
    23641 ttcagctttt gttggtgatg attggcggaa aatttatcac tatgctctgg aacataactt
    23701 tcggttttta agttatggtg acagttcctt actgctgaaa taatttttca agaaagttct
    23761 aaaagctaaa acagttaaaa caaaaatcta tggaaaattt attgactgat tcccctttgt
    23821 ttgatcaatt ccctgatatt caatattttt tcactaccag aaaaggaggt tttagttatg
    23881 ggaattatgc tacttttaat ttgagtccgt atgttgggga cgaacctcaa tgtgttgctc
    23941 agaatctcaa caaattagcg gaaactcttt cattgccaac acatagaatt tttttccctc
    24001 accaaaatca tgggaaattc atccatgaaa ttgatgaaac ttttttgcag ttgacccccg
    24061 aagaacagaa aaatcgcctt gccaaggtag atggcttgta cacaaatttg aaaaatgttt
    24121 gtattagtgt cactactgcc gattgtgtgc cattattgtt ttatgatcct gttaaaaagg
    24181 cagttgctgt ggctcatgct ggttggagag gaactgttca gcaaattgca gcaaaaatgg
    24241 tagctacact tgtacggaaa tacggttgtt ctcctaaaaa tattcgtgtg gttatgggtc
    24301 ctgccatttc ggaacaaaaa tatgaagtag gaaaagaaat tttactaact tttataattg
    24361 ctggctttga aacatcagaa attttcattc aacaagaaaa taagattttt ttaaatctct
    24421 ggaaagcaaa cgaaatgatt ttgttgggga gcggcgtttt aaaatctaat atcgaaatca
    24481 aggaattatg tacattcaca aattcaagcg atttcttctc agcccgtcga gagggcacgt
    24541 actgtggaag aatgttgaac ggaattattt tacgataaaa acatttttaa tttattattt
    24601 tgaaaatatc aatctcatca ctggttcaca gtatttgtcc tgatttagtt ttgggtatta
    24661 ttgaagggaa agtcaaaaat tcaccaactt ctgcgttgtt atggcaagag gtggaaaaag
    24721 aaatggaaga gattctttca aaatattcat tgaaacaaat cagtaaacgc cctgctattt
    24781 tagccacaag acaagcttat aaaaaatttg gcaaagatcc ttcacgttat cgaccttcag
    24841 cagaggcttt atgcagacga atctgtaaag ggaattccat ttatcaaatc aatacactgg
    24901 tcgatattat caatcttgtt tctattcaca gtggtttttc aattggtggt tttgatgcag
    24961 acaaaattca aggaaatctt ttactcgatg tgggaaatga aaatgatgag tttgaagcta
    25021 ttggcagagg aattttaaat gttgccggtc ttccgctttt tcgagacgaa gtaggtggta
    25081 ttggaacacc taccagcgat aatgaacgaa caaaaataac tatggaaact aatcatttat
    25141 tgatgattat caacgcttat ggaggtaaaa ctggacttga aaaaactata gattatgcag
    25201 tggacttact caaaaaatat gctgaaatga cggcatataa tattttttta tcctaaaact
    25261 gaataacatt ttgtattagt gggtacaaaa atatcggtgc tacgcacctt tgttctttga
    25321 tgatatatcg ataaatatat caatttgata taaaatttat cagataccaa aacaaaatac
    25381 ttgattgact tttaatactt tatagccttt tttattaaac tttctaaata cttattttct
    25441 caatgcaatt cttggtttag aaataaaagt acctgaaaat aaaccaattg ttgtgttaaa
    25501 cgcggataat cataaataat tctacaattt cctggtcact gctatggttg gaatactttc
    25561 ctgtttaaaa cgattaatag cagttacaac aaaagtataa tgtcctttca gatgaacatt
    25621 taaagctcgt aaatcaagac aattatctgt agttcgagca ataatattag caggatcgtt
    25681 caaatcacca actttctttc ctttaaaagc atacaccaca taataagcta cttgacaacc
    25741 gccttcttct tctacttcgt cccacaataa ataagcagca tctccatctt ttacaattct
    25801 tatattttgt ggttgagcgg aaggttcccc tacaatattt ttattcacgg gacaaatggc
    25861 aggatactta taaaaatttt gctgtaaact atcattcaat cctagcctgt tttgtaaaaa
    25921 agttttggca ctaaaaaaca cagctccttc cacttgagga tattgttgat taagccttag
    25981 ttgacgaata atttcatttc ctttcgacca tgccgaattt ttattgctcc ctaatgaaga
    26041 cggaatcaaa cctggatata aatttttacc ataagaattt tcgctccacc attttaaaag
    26101 cacttcataa tcagctactt tcttgccaat ttcaaaatac aactgaggaa ctacataatc
    26161 tatattacct tcttttaacc atttcaaaat gtctgcataa agatcatcat aattttgaat
    26221 gccagcacgt gtggcagaac ctcgaggatc aacattattg tttcgccaaa caccaaaagg
    26281 acttacacca aattctaccc atggttttaa atttttaata gtttgctgaa cttcagcaat
    26341 gaccatattc acattgttcc ttctccaatc agctttttga tcaggagcaa aaccacgagg
    26401 attttttctg aaagtatctt catcaggaaa ttcttctcct tttactggat aagggtaaaa
    26461 atagtcatca aaatgaattc catcaatatc ataatgttca acaatttctt ttatgacgtt
    26521 atttaaaatc tgtcgggttt cgtccagacc tggattaaaa taatatttgc cgccgtaacg
    26581 aacaaacaat tccggttttt gataaaacaa atgattaggc gacaatactg acaaattatc
    26641 gttcattgta acacgataag gattgatcca ggcatgaact tccatgcaac gtttatgtgc
    26701 ttctgtaata ataaattgta aaggatcata gaaaggattt ggagcaaagc cttgtttgcc
    26761 tgtcaaccaa tgcgaccacg gatcaatgga agaaggataa aaagcatccg ccataggccg
    26821 aacctgaaga ataatagcat tgatattgtt ttgagcaagt ttgtccagca tattggttat
    26881 ttctattcgt tgttcacggg gtgtaagtcg gttagaacta ggccaatcaa tattggctac
    26941 tgtggcaatc cagactgctc gcatttcacg ctttggctgg gcataaat
//
LOCUS       DAOSAS010000005        23977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_1519_length_23977_cov_13.033944, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000005 DAOSAS010000000
VERSION     DAOSAS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 23977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23977
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1519_length_23977_cov_13.033944"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PLR72_00530"
     CDS             complement(<1..748)
                     /locus_tag="PLR72_00530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOK35731.1"
                     /translation="MQKRYFDAFQYISEQAYTTEKYILPFIQTIKKINSSTRVLEIGC
                     GEAGNLKPFLDAGCKCTGIDISSVKIEKGKEFFTTHPLVENLTLICRDIYQINNELHD
                     FDIIILRDVIEHMPDKEKLLDLLKEWLAIDGIIFIAFPPWQNPFGGHQQICESKFLSV
                     LPYFHLLPVSAYKNILRFFGEKEGKITGLLEIKQTGISLENFEQLVKKNDFQILKKVL
                     YFINPNYEIKFGLKPRKLFSVFADIPYFRNF"
     gene            complement(757..2289)
                     /gene="gltX"
                     /locus_tag="PLR72_00535"
     CDS             complement(757..2289)
                     /gene="gltX"
                     /locus_tag="PLR72_00535"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOK35732.1"
                     /translation="MNDDLTNVRVRFAPSPTGPLHIGGVRTALYNFLFARKNKGTMLL
                     RIEDTDSNRFVPGSEEYIIESLNWLGIEIDEGIGAKTAGKYGPYRQSERKEIYKQYVR
                     QLLNDGKAYLAFDSAIELAAKRAEIENFQYDANTRNEMCNSLTLPPEEVKARIANGEQ
                     YVVRIKIEPNEEITVNDLIRGEVTIHSSVIDDKVIYKSVDELPTYHLANVVDDYLMKI
                     THVIRGEEWLPSSPLHVLLYRYLGWEKEMPQFAHLPLLLKPDGKGKLSKRDGDRLGFP
                     VYPLNWVDPKTGEVYKGFREAGYFPEAVVNFLALLGWNPGTEQEIFTMEELIENFSLD
                     RVSKSGAKFDYEKARWFNHKYLQMKSDEELADTFQRMLVEKGFVEDSDRVLKIVSLIK
                     DRANFITDLWEQSHYFFEAPTQYDPKAVKKCWKPEIVPHLIELADFLENIQDFSAENL
                     EIEIKKWLAEKNYPLGNLMNALRLSLVGELKGPSIFEIVSLLGKEETIKRIQKTIKEI
                     NM"
     gene            complement(2502..3950)
                     /locus_tag="PLR72_00540"
     CDS             complement(2502..3950)
                     /locus_tag="PLR72_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOK35733.1"
                     /translation="MGDNLKQKTLDALTWSAIDRFGQQIIQVIIGIIVARLLSPDDYG
                     LMGMIMIFAALSFVLVESGFGQALIRKKDASEIDFNTIFYFNILISIALYVLLYFLSP
                     AIARFFHQPQLIKIGRIIFISLLFNAFYLVPFTQKVKEMDFKSIAKINIFSVFLSGSA
                     GVTLAILKFGVWALVIQQISFHFFRMIGYHFFVKWKPKKLFSFSIIKEFWSFSVHILG
                     SSILTVIFNNLYVFILGKYYQRKDVGYYSQANKLSETVNFTFQQILGSTYSMFSQIQD
                     NNERLNRIFKVMIKRTALVTIPVIFTFIVIAYSLIYVLLSAKWLPSVPYVQILCLAAL
                     FNPFYTLNINVLNARGKSKTTFRIEIIKKGLILLSAIICFKWGIFIMLGAYAFINFLA
                     YLLSLFSVKKEIHLTIKSQIETLVPGLLIGLILALFGAGIAWLCKNLFVEFFVQLAIV
                     CLLYFFIIRKYQNDFYNEAIEFVQSKVKAFKK"
     gene            complement(3975..4859)
                     /locus_tag="PLR72_00545"
     CDS             complement(3975..4859)
                     /locus_tag="PLR72_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOK35734.1"
                     /translation="MNIKSITKKNWITIREYVLITFGLLMYAFAWKGFLLPHQITGGG
                     VTGIGAIVYYATGIPIFLTYFSINLILLIAALKIIGFKFSVRTIYGVIAITLFFAVIP
                     QAKTGTFVAPNDNFMACVLGGLISGTGLGIVFLNNGSSGGTDIIAKIVNKYRNITLGR
                     VLLYCDVLIICSSYFFSFGSLEKVVYGLTNMAIANLSIDWVINGVRQSVQFFIFSKEY
                     DKIATRINEEIHRGATILEGTGWYSKQPVKVVTVIARKNESQRIFRIVKEVDPDAFVS
                     QSSVIAVYGKGFDVMKTK"
     gene            complement(4890..7646)
                     /gene="leuS"
                     /locus_tag="PLR72_00550"
     CDS             complement(4890..7646)
                     /gene="leuS"
                     /locus_tag="PLR72_00550"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOK35735.1"
                     /translation="MEYNFKEIEVKWQNYWNENNIYKTEIDLTKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRMKGFNVLHPMGFDAFGLPAEQYAIQTGQHPAITTKK
                     NIERYREQLNKIGFCYDWSREIRTCDPDYYKWTQWAFIQMFKHYYDEKLQKAQPITLL
                     IEHFEKYGTENLTAVGTIELNFTADEWKAMSEKAQQEILLNYRIAYLADTKVNWCPAL
                     GTVLANDEVSEGVSIRGGYPVEQRVMRQWCLRVSAYAQRLLDGLEQIDWSESLKETQR
                     NWIGRSEGAELRFTIKGKNVSFDVFTTRPDTIFGVTFMVLAPESELVKICTTPEQRKE
                     VEEYLEATKRRTERERIADRQVTGVFTGSYAIHPITGKEIPIWISDYVLAGYGTGAIM
                     AVPAHDSRDYAFAKHFNLPIVPLIEGCDVSNESFDAKEGIMINSEFLNGLSVPEAIEK
                     IKKYITKKGIGRVKVNYRIRDAIFSRQRYWGEPFPVYYKDGMPYVLDEKELPLVLPEI
                     DKYLPTENGEPPLARAKNWKTKEGYPLEVCTMPGFAGSSAYYLRYMDPHNDEALVSRE
                     ADEYWRDVDLYLGGTEHATGHLIYSRFWNKFLFDIGVVCEEEPFKKLINQGMILGRSN
                     FVYRIKDTNTFVSYHLKDQYDTTPIHVDINLVDNDILDIEKFRDWNPEYKNAEFILED
                     GKYICGWAIEKMSKSMYNVVNPDDIIEKYGADTLRLYEMFLGPIEQSKPWDTKGIDGV
                     YRFLKKLWNLFYKEEQFLVNQEPATLEELKTLHKTIKKVTFDIENFSYNTAVSAFMIC
                     VNELTALNCHKKEILEPLVILLAPFAPHIAEELYHAMGNEGTIFNASFPPWNENYLIE
                     NKVKYPISFNGKVRFTLELSANMETKEIEKVALEHPQTIKLLAGKTPKKVIIVPGKIV
                     NIVF"
     gene            7862..8494
                     /locus_tag="PLR72_00555"
     CDS             7862..8494
                     /locus_tag="PLR72_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOK35736.1"
                     /translation="MDFKVPEPTLRRLPWYLAYAKLMLEKGETFLSSTQIANDIAIDA
                     SIVAKDLSFVKISGRTRVGYDIKELIYVLERFLGFNVSHKAFLFGVGNLGGALMHDNG
                     LEQFGLKIVAGFDVKYELIGTSINKIPIHHIDHFPELQKQTGVIIGILTVPVDKAQKM
                     AELIVDGGIKAIWNFTPFRIRVPENVVVQNTSIYAHLAVMFNRLNAILGK"
     gene            8517..9128
                     /locus_tag="PLR72_00560"
     CDS             8517..9128
                     /locus_tag="PLR72_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOK35737.1"
                     /translation="MKIICIGENYPEHNKELNTTNPGEPVVFIKPDTAVLKNNKPFYI
                     PDFTNELHYETELIVKINRLGKNIAAKFASRYYNEIGLGVDFTARDLQRKLRAEGKPW
                     EICKAFDNAAVIGNFLPISYFKNLQDISFHLNINEKTVQRGNSKDMIFSIDELIAYVS
                     RFFTLKIGDILFTGTPAGVGKVSVGDHLEGYIFEEKMFDFFVK"
     gene            9211..13017
                     /gene="porU"
                     /locus_tag="PLR72_00565"
     CDS             9211..13017
                     /gene="porU"
                     /locus_tag="PLR72_00565"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOK35738.1"
                     /translation="MRIKHFLFLFFPLLLFVGKSEAQTTFSFAQKINWIGVQKWFADS
                     SYINVISFENACYPSDNFLPYFNYKQEMEPSFSYEASITNPVWIPVAPDELKIVEQYS
                     FVAEPEIKSNVAIEKGVSFFSVNILPFVEREGQYFKLLSFDLVIKKTSAPQKTKASTL
                     HTYATSSVLAQGKFVKIKVANNGIYKITYDDLVKMGINPTNVRVFGYGGGVLEQSFLK
                     NKIDDLPEVAIWMEKGKDGIFNTGDYILFYAQGPVKWYYDEAKSKFVHVNNPYSLYGY
                     YFITSDAGVGKKIEEKTIEVPSDAVIHQITEFTDYQVYEKDQVNLCSSGKEFYGEIFN
                     TRTSYEFSFNFPNILYTNTTKVTVDVAATSSANSSFTISLNNTQSKSLTVQKISGDHY
                     EMGKAATGSYIFTPNSETLNFKIDYNKSTSTSTGYLNYIEVNARRRLQMSGSVMQFQN
                     VDYLNKSSNYNNYILSNINSNIQIWDITDPQNITRVPFTMVNDNTLQFTAPGYELHTY
                     LAIDPTAGSSFSTPETVGKVVNQNLHGLQPVDMVIIVHPNFKSQAEKLAQTHREKDNL
                     RVAVATTEEVYNEFSSGTPDATAYRWIMKMFYDRALNANRPDDLPKYLLLFGRGSYDN
                     RGIITNSGDNLILTYQADNSLVITSSYVTDDYFGFLDDNEGIDLTSNLLDIGVGRFPV
                     STDQQATDIVNKTVNYIQNKGKGIWKNQLCFLADDGDYALHMTQADSIATSIARLYPG
                     YQINKIYLDAFVQEVNASGQSYPVAKSRFQNLLRSGMFLLDFMGHANSGGWTNEAILT
                     TADIKNLTNQHLAVWMAATCDFLQFDIKPISAGEHVVLNPSGGGIGLLSAARPVYASQ
                     NFYINKYFVESLFKKENENHLRVGDAIRMAKNKIGAEINKLCYVYVGDPAVMLNYPDK
                     FSVVTSKINGNTQLGNDTLKALSVTTIEGYISDNVGNKIPDFNGKIEVVVYDKLQKIT
                     TLNNEKDGALTYYDRPNTLFAGKTDVKNGEFSFTFMLPKDIKYNYGTGRINYYAYDDD
                     HDYEAQGYFENFLVGGSSQSYVNDTIGPKLTIYLNSPEFVSGGKVNETPLFVAHLEDE
                     HGINAVGSGIGHDLVLIIDANPLTSYILNDYYQSEENSYTSGSVSYKLPELKEGKHTL
                     TFRAWDLLNNSSTQTLDFEVVKGLKPVIFSVYTYPNPVQQRTKIVINHDRPETQLITT
                     VEIFDITGRKIWSFSQSSIDDITWDLKGADDKRVQNGIYLYRVSIKTPNSEMTSKTNK
                     IIVVGQ"
     gene            13090..14217
                     /gene="porV"
                     /locus_tag="PLR72_00570"
     CDS             13090..14217
                     /gene="porV"
                     /locus_tag="PLR72_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOK35739.1"
                     /translation="MKKRCSILSVCFFLAFYGFSQKNNPVITAVPSLTIAPDARAGAM
                     GDVGAATQVDVNSQYWNPAKYAFMESSAGVAFSYTPWLSKLVSDINLYYLAGFWKFDP
                     LQSVSASLRYFSLGDIDIMDEFGYPQGAAHPNEFAFDIAYSRLLSTKFSGSVAFRYIR
                     SDLNSGVNLSGGTEMYPGNAFAADIAGYFKTPIYMSSGDGNLAFGFNISNIGSKISYD
                     EKETSYFIPTNLRLGGSFEYPIDDYNKISFNADINKLLVPTQDTMSIAEYSDISSISG
                     IFRSFSDAPGGFSEELQEVMWSVGAEYVYNKQFSVRAGYFHESQNKGNRKYFTVGAGF
                     KLNVFELDAGYVIATNQSNPLDQTLRFTLAFDINGLKNLIK"
     gene            14304..14783
                     /gene="ispF"
                     /locus_tag="PLR72_00575"
     CDS             14304..14783
                     /gene="ispF"
                     /locus_tag="PLR72_00575"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOK35740.1"
                     /translation="MNFRIGFGYDVHPFKEERELWLGGIKIDYPYGLAGHSDADALIH
                     AICDALLGAACLGNIGIHFPDTSDEFKNIDSKILLDRTLQLIRKNGYEIGNIDCTVCA
                     EEPKLNPYIPAMQKCLSKIMNIKIDDISIKATTSEKMGFVGRKEGIAVYAVTLIYKK"
     gene            complement(14804..15379)
                     /locus_tag="PLR72_00580"
     CDS             complement(14804..15379)
                     /locus_tag="PLR72_00580"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOK35741.1"
                     /translation="MKFELPKLPYAYNALEPVISEKTISFHYGKHHQTYVNNTNNLIV
                     ATEFENADLETIIKKTSGALFNNAAQVWNHNFYFLALTPDKGTKPSDKLLKAIESGWG
                     SLDNFKTEFNKLAIALFGSGWTWLVKDKDGKLAIVNESNAGNPIVQGLTPILTFDVWE
                     HAYYLDYQNRRADYVAALWELINWEVVSGKY"
     gene            15654..16253
                     /locus_tag="PLR72_00585"
     CDS             15654..16253
                     /locus_tag="PLR72_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOK35742.1"
                     /translation="MDSEIKLVISGLVYNQNIVGTYGLILSEESGNRRFSVMIGEPEA
                     QSIALKLNNKKLPRPLTHDLIKNILDAFEVTLVKVLISDMINDVFYSELHIKKDNKIL
                     VVDSRTSDAIALAVRCNCPIYIKSEILDMVGVVIEPTNEEEETIPEKTEYDKISDSQL
                     DRLSLEELDELLSMAVNEEKYELAVHIRDAINRKKSSKQ"
     gene            16371..>17448
                     /locus_tag="PLR72_00590"
     CDS             16371..>17448
                     /locus_tag="PLR72_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HOK35743.1"
                     /translation="MSLKFRLVILSFLQYFIWGSWLISLGSYLGNVLGFDGIQIGSIF
                     GAMGIVSLFMPGLLGIVADRWIPAQKLLGICHLLGAIFLILASFQREYFPFYILMFLN
                     VMFYMPTISLSNAVAYNALEEAHYDIVKDFPPIRVWGTIGFICAMWTVDLTGFKYSQV
                     QLYVAATAALLLGIYSFTLPPCKINKTQEKKSLVDALGLRAFSLFKQKTMAIFFIFSM
                     LLGASLQITNGFGDLFLRSFEKMPEYVNSFGVKHSVILLSISQMSETLFILAIPFFLK
                     KFGIKKVMLISMIAWVFRFGLFGLGNPGERLWMLILSMIVYGMAFDFFNISGSLFVEQ
                     STDSSIRASAQGLFMIMTNGLGATM"
     assembly_gap    17449..17548
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17549..17776
                     /locus_tag="PLR72_00595"
     CDS             <17549..17776
                     /locus_tag="PLR72_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK35744.1"
                     /translation="ASAQGLFMIMTNGLGATMGSYLAGMVVDKFVTHAAVPSWSTAWF
                     IFSGYALVVAILFAILFKYKYEPEKSPEISH"
     gene            complement(17822..19360)
                     /gene="dnaB"
                     /locus_tag="PLR72_00600"
     CDS             complement(17822..19360)
                     /gene="dnaB"
                     /locus_tag="PLR72_00600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOK35745.1"
                     /translation="MAEKSKYIKATTPSTVQISPNEYGKMPPQAPELEEAVLGALMIE
                     KDAYFQVSEILKPEYFYKTAHQKIFEAIVTLAYHQEPIDMHTVTEQLRKNGSLEEVGG
                     PYYITLLTAKVSSAAHLEYHSRIIVQKYLARELIRISNEIQTKAFDEKTDVDDLLEES
                     EAMLFEVSQRNLKKEVLPISSVLDEAIQRIQAAASREGASGIPTGFHSLDKITSGWQK
                     SDLIIIAARPSMGKTAFVLSMAKNMAMNYNIPVGIFSLEMSNIQLANRLIMNICEIDG
                     SKIKNGELEPYEWEKLDKNLKDLIDAPIYIDDTPSLSIFELRSKARRLVKEHHVQCII
                     IDYLQLMNASGMNFGSREQEVSTISRSLKGLAKELDIPIIALSQLNRSLEGRSGLEGK
                     RPQLADLRESGAIEQDADVVCFIHRPEYYHITEDRDGNSLLGIAEIIIAKHRNGATDM
                     IQLKFKSEFAKFLNLDDFDAQEEILNEYKVIPSKINKTEKSTASRFSSLDSFLYDNSE
                     EVPF"
     gene            19732..20040
                     /locus_tag="PLR72_00605"
     CDS             19732..20040
                     /locus_tag="PLR72_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOK35746.1"
                     /translation="MEDEKNQISIELSAEVAEGIYSNLAIISHSSSEFVIDFIRIMPG
                     IPKAPVKSRIILTPEHAKRLLFALEDNIAKYEQQNGKIKIAENTFIPPFPMGFGGGEA
                     "
     gene            complement(20087..20623)
                     /locus_tag="PLR72_00610"
     CDS             complement(20087..20623)
                     /locus_tag="PLR72_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOK35747.1"
                     /translation="MSRLDFTFYAKDAVTVAENLLGKILIRRWEDGSFSRYIITETEA
                     YFGEEDKACHASKGRTPRTEIMYASGGHVYVYFIYGMYWMLNVVTGPENHPEAVLIRG
                     LNDIIGSGKVGRELKLDRSFYGENLITSQRIWIENTNEKIVFKSAPRIGINYAGEEWI
                     NKPWRFILEREKNPPSLK"
     gene            20894..21349
                     /gene="rplM"
                     /locus_tag="PLR72_00615"
     CDS             20894..21349
                     /gene="rplM"
                     /locus_tag="PLR72_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOK35748.1"
                     /translation="MDTLSYKTISANKATVQKEWVLIDATDMVLGRMGSKVAKLLRGK
                     YKPNFTPHVDCGDNVIIINARKVKLTGDKWTDRVYLRYTGYPSGQRETTPAKIMEKDP
                     EKLIRRVVKGMLPKNRLGAQLLKNLYIFPDEEHNMQAQKPKQIDLNSLK"
     gene            21355..21741
                     /gene="rpsI"
                     /locus_tag="PLR72_00620"
     CDS             21355..21741
                     /gene="rpsI"
                     /locus_tag="PLR72_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOK35749.1"
                     /translation="MEVINALGRRKTAVARIFVSEGNGKITINKQDLSQYFPSPLLQY
                     IVKQPLNKLNVAEKYDIKVNLNGGGFKGQAEALRLAIARSLVKINPEDKKLLKSEGFL
                     TRDPRIVERKKPGRPKARKRFQFSKR"
     gene            21907..22743
                     /gene="rpsB"
                     /locus_tag="PLR72_00625"
     CDS             21907..22743
                     /gene="rpsB"
                     /locus_tag="PLR72_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOK35750.1"
                     /translation="MSRTNFEELLEAGCHFGHLKRKWNPAMAPYIFMERNGIHIIDLY
                     KTIAKIDEAAEALKQIAKSGRKILFVATKKQAKEIVAEKAKAIGMPYITERWPGGMLT
                     NFPTIRKAIKKMATIDKMMTDGTFDNISKREKLQITREREKLEKNLGSIADLTRLPAA
                     IFVVDVMKEHIAVKEAQRLEIPIFAIVDTNSDPTGIDYVIPANDDATKSIEVILNALV
                     AAIQEGLDERKLMKVEAEASEAPVVKEKKSRINRRPRTQKEDEEALKANVLNKFIKDE
                     EE"
     gene            22881..23699
                     /gene="tsf"
                     /locus_tag="PLR72_00630"
     CDS             22881..23699
                     /gene="tsf"
                     /locus_tag="PLR72_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOK35751.1"
                     /translation="MAVTMNDITKLRAMTGAGLMDCKQALIEANNDFEKAIEIVRKKG
                     QAIAAKRSSREALEGCVLAEAKDDFAVALSLKCETDFVARNADFIALTNSILQAALEN
                     RPTTKEELLALKINGRTIADLVIERSAVTGEKVELEDYVFVKGGSTAAYTHFGNRLAS
                     IVAFKEKDVDPKVAHEIAMQIASMNPIAIDRASVPQKMIETELEIAREKAREEGKPEN
                     MIDKIAEGRLNKFFQEAVLLEQTSIMDNKTIIGNYLKAHNATVIDFKRISLNQE"
BASE COUNT         7906 a         3891 c         4061 g         8019 t
ORIGIN      
        1 aaaaatttct aaaataagga atatcagcaa aaacagagaa aagtttacgt ggttttaatc
       61 caaacttaat ttcatagttg ggattaatga aatataacac tttctttaga atttgaaaat
      121 catttttttt caccaattgc tcgaaatttt ccagactaat gcccgtttgt ttgatttcta
      181 ataaacctgt gatctttcct tctttttcac caaaaaatct caaaatattt ttatatgctg
      241 aaacaggtaa caaatgaaaa taaggtaaaa cggacaaaaa tttactttca caaatttgtt
      301 gatggccgcc aaaagggttc tgccacggtg gaaaagcaat aaaaataata ccatcaatag
      361 ccagccattc ttttaacaaa tcgagaagtt tctctttgtc aggcatgtgt tcaataacat
      421 cacgcaaaat aataatgtca aaatcatgca actcattgtt tatttgataa atatctcgac
      481 agattaatgt caaattttct accagcggat gcgttgtaaa aaattccttg cctttttcta
      541 ttttaacaga actaatatct atgcctgtac atttacaacc agcatcaaga aaaggtttta
      601 gatttccagc ttcaccacaa ccgatttcaa gaacgcgagt ggacgaattg atttttttta
      661 tggtttgaat aaaaggtaaa atatattttt cagtagtata ggcttgttcg ctaatatatt
      721 gaaaagcatc aaaataacgt ttctgcattc ggctttttac atattaattt ctttgatggt
      781 tttttgaatc cgctttatcg tttcttcttt tcccaatagc gacacaattt caaaaatcga
      841 aggtcctttt aattcaccta ccagcgacaa tcgaagagca ttcattaaat ttccaagcgg
      901 ataatttttt tctgccagcc attttttaat ttcaatttca agattttcag cagaaaaatc
      961 ttgaatattt tcaaggaaat cagccagttc aattaaatgt ggaacaattt ctggtttcca
     1021 gcattttttt accgcttttg gatcatattg cgttggagct tcaaaaaaat aatgagattg
     1081 ttcccataaa tcagtaataa aatttgcccg gtctttaatt aaagagacaa ttttaagcac
     1141 tctgtccgaa tcttcaacaa atcctttttc aaccaacatt cgttgaaaag tatctgccaa
     1201 ttcttcatcc gatttcattt gcaaatactt gtgattgaac catctggctt tttcatagtc
     1261 aaattttgct ccgcttttgc tgacccgatc cagcgaaaaa ttttcgatta attcctccat
     1321 tgtaaaaatt tcctgttccg ttccaggatt ccagcccaac aaagcaagaa aatttacaac
     1381 cgcttcagga aaataaccag cttcacgaaa acctttgtaa acttcgcctg tttttggatc
     1441 aacccagttg agtggataaa caggaaatcc gagtctgtcg ccatctcgtt tgctgagttt
     1501 gcctttgcca tcaggtttga gcaaaagcgg aagatgagca aattgcggca tttctttttc
     1561 ccagccaaga taacgataca gcaacacatg taaaggcgaa gaaggcaacc attcttcacc
     1621 acggatgacg tgggttattt tcattaaata atcatccact acgtttgcca gatgataagt
     1681 aggcaattca tctaccgatt tataaattac tttatcgtca ataacagaag aatgaatggt
     1741 tacttcccca cgaattaaat catttactgt aatttcttca ttcggttcaa ttttgatgcg
     1801 aacaacatat tgttctccat tggcaattct tgctttaact tcttcaggag gcaatgttaa
     1861 agaattgcac atttcatttc gtgtgttcgc atcatactga aaattttcta tttccgcccg
     1921 ctttgctgcc agttcaatag ctgaatcgaa agcaagataa gcttttccgt catttaataa
     1981 ttgcctgaca tattgtttat agatttcttt tctttcgctt tgtcgataag gaccatattt
     2041 tcctgccgtt ttggcaccaa tgccttcatc aatttctatt cccagccagt ttaatgattc
     2101 aatgatatat tcttctgaac cgggaacgaa tctgtttgaa tcggtatctt caattcttaa
     2161 taacatggtt cctttgttct ttctggcaaa aaggaaattg tacaatgccg tccgcacacc
     2221 gccaatgtgt aaaggtccgg ttggacttgg tgcaaatctt accctcacgt ttgttaaatc
     2281 atcattcatt ttttctattc aaaaatttgg tgactttgca aagttaataa atttcactta
     2341 taagaaggtg atttttaatt gttttttctt aatgaaacat tttgtctggc cagcaataaa
     2401 aacgaaactc ttaatttgca agaaagcaca atttctgttc gtttttgttg cagtcagagg
     2461 cttgctaata ggcaacaagt tttaaaaaaa caggtgaaga attatttctt gaaagctttt
     2521 actttacttt gaacgaattc aatggcttca ttgtaaaagt cattttgata ttttcttata
     2581 atgaaaaagt aaagcaaaca aacaattgcc aattgcacaa aaaattctac aaaaagattt
     2641 ttgcacagcc acgcaattcc tgccccaaac aaggcaagaa ttaaaccaat cagtaaaccg
     2701 ggaacaagcg tttcaatttg acttttaatg gttagatgaa tttctttttt tacggagaaa
     2761 agtgaaagta aataagccag gaaattgata aaagcataag cgcctagcat aatgaatatt
     2821 ccccatttaa agcaaatgat cgctgaaagt aaaatgagtc cttttttaat gatttcgatc
     2881 cgaaaagttg tttttgattt tcctcttgca tttaaaacat tgatattgag tgtataaaaa
     2941 ggattaaata atgctgccag acacaaaatc tgaacataag gaacggaagg caaccatttt
     3001 gctgaaagca gaacataaat taaagaatag gcaattacaa taaaggtaaa tatcacagga
     3061 atagttacca aagcagttct tttgatcatt actttaaaga tacgattcaa tcgttcatta
     3121 ttatcctgaa tttggctaaa catggaataa gtgcttccca atatttgctg aaaagtgaaa
     3181 ttgactgttt cacttagttt gttagcttga gaataatacc caacatcttt tctttggtaa
     3241 tatttaccga gaatgaaaac gtataaatta ttgaaaataa ctgttaggat agaagaacct
     3301 aaaatatgaa cactaaaact ccagaattct ttaataatag aaaaagaaaa caacttttta
     3361 ggtttccatt ttacaaaaaa atgataacct atcattctaa aaaaatgaaa agaaatttgt
     3421 tgaatcacta atgcccatac gccaaatttc aaaatagcca gtgttacacc ggcacttcca
     3481 cttaaaaaaa cggaaaaaat attgatttta gctatagatt tgaaatccat ttctttcact
     3541 ttttgtgtaa atggtaccag ataaaaagca ttgaacagca aggaaataaa aataattctt
     3601 cctattttga ttaattgtgg ctgatgaaaa aagcgagcaa tggcaggtga tagaaaataa
     3661 agcagaacat ataatgcgat ggaaataaga atgttaaaat aaaaaatggt attaaaatca
     3721 atttcagaag cgtctttttt acgaattagt gcttgcccaa aaccgctttc tactaacaca
     3781 aatgacagtg cggcaaaaat cataatcatt cccatcagtc cataatcgtc aggcgacagc
     3841 aaacgggcga cgatgatgcc aataatgact tgaatgattt gttgaccaaa tctgtcaata
     3901 gcactccagg taagagcatc gagggttttt tgttttaaat tatcacccat ttgttttgac
     3961 aataaagata agatttattt tgttttcatc acatcgaatc ctttgccata aacggcaatg
     4021 acagacgatt gcgaaacaaa agcatcagga tcaacttcct taacaattcg aaaaattctt
     4081 tgcgattcat ttttgcgagc aataaccgtc actactttaa ccggttgttt agaataccag
     4141 ccagtacctt ccaaaatagt ggctccgcga tgaatttctt cattgattcg cgtggcaatt
     4201 ttatcatatt cttttgaaaa aataaaaaac tgaaccgatt gccgtacacc attgataacc
     4261 caatcgatgg aaagattagc aatagccatg ttggttaaac cgtaaaccac tttttccagg
     4321 ctaccaaatg aaaaaaaata agaagaacag ataatcaaaa catcgcaata cagcaacact
     4381 cttcctaatg tgatatttct atatttattc acaatttttg caataatgtc tgttccaccc
     4441 gagctgccat tgttaagaaa aactattcct aatcctgttc ccgagattaa tcctcccaga
     4501 acgcaagcca taaagttatc gtttggagca acaaaagtgc cagttttagc ctgtggaatt
     4561 acagcaaaaa atagcgtgat agcaataacg ccataaatag tgcgtacact aaatttaaag
     4621 ccaatgattt ttaatgctgc aataagcaaa attaaattaa tgctgaaata agtcagaaaa
     4681 attggaattc ctgtggcata ataaacaatt gctccaatac cggtaacgcc gccacccgtg
     4741 atttgatgag gcaataaaaa acccttccac gcaaaggcat acatcaataa tccgaaagta
     4801 atcagaacat attctcgaat agtaatccaa ttttttttag tgatggattt gatattcata
     4861 ttcttttttg ctaaagaaag gcttactttt taaaaaacaa tattgacaat tttgcctgga
     4921 acaatgatta ctttttttgg tgttttccct gccagcaatt taatggtttg aggatgttcc
     4981 aatgcaactt tttcaatttc ttttgtttcc atatttgcag acaactccag tgtaaaacgt
     5041 acttttccat taaaagagat ggggtacttc accttatttt ctatcaaata attttcattc
     5101 catggtggga aagaagcatt aaaaatagtt ccttcatttc ccatagcatg ataaagttct
     5161 tctgcaatat gtggagcaaa aggcgccaac aaaataacca acggttccaa tatttctttt
     5221 ttatgacaat ttaacgctgt tagttcattt acacaaatca taaatgctga aacagcagta
     5281 ttgtacgaaa aattctcaat atcaaaagta acttttttaa tggttttgtg aagcgttttt
     5341 aattcttcta aagtagctgg ttcttggtta accaaaaatt gctcttcttt gtaaaacaag
     5401 ttccataatt ttttcagaaa acgatatacg ccatcaattc ctttcgtgtc ccacggtttg
     5461 gactgttcaa tcggtcccaa aaacatttca tacagtcgca acgtgtcagc accatatttt
     5521 tcaataatat catcaggatt aaccacattg tacattgatt tagacatttt ttcaattgcc
     5581 cagccgcaga tatatttgcc atcttccaga ataaattcgg catttttata ttctggattc
     5641 cagtctctaa atttttctat gtcgagtata tcattgtcca ccagatttat atctacatga
     5701 atgggagttg tatcatattg atctttaaga tgataggaaa caaatgtgtt ggtatcctta
     5761 attctataga caaaattact tcttcccaga atcattccct gattaatcaa ctttttgaaa
     5821 ggttcttctt cgcaaacaac accgatatca aataaaaatt tgttccagaa tcggctataa
     5881 atcaaatgac ctgtagcatg ttctgttcca ccgagatata aatccacgtc tcgccagtat
     5941 tcatctgctt cacgacttac caatgcttca tcattgtgag gatccatata tctcaaataa
     6001 taagcagaag aacctgcaaa accaggcatg gtacacactt ccagtggata accttctttt
     6061 gttttccagt ttttggctct tgccagtggc ggctcaccat tttcagtagg aagatattta
     6121 tcaatttcgg gaagaaccaa tggaagttct ttttcgtcca gcacgtaagg catgccatct
     6181 ttataataaa ccgggaaagg ttcgccccaa tatcgttgac gactgaaaat agcatctcgg
     6241 atgcgataat ttactttgac gcgacctatt cctttttttg taatatattt ttttattttt
     6301 tcaatcgctt ctggtacaga aagtccgttc aaaaattcag aatttatcat aattccttct
     6361 ttagcatcga agctttcgtt gctgacatcg caaccttcta tcagaggaac aatgggcaaa
     6421 ttaaaatgtt ttgcaaaagc atagtcacgg ctgtcatgtg caggaacagc cataatagcg
     6481 ccggtgccat aaccagcaag aacataatca gaaatccaga taggaatttc ttttccagtg
     6541 ataggatgaa tggcataaga gccagtaaag acaccagtta cttgtctatc ggcaattcgt
     6601 tctcgctcgg ttcttctttt tgttgcctct aaatattctt ctacttcttt tctttgttca
     6661 ggtgtagtgc agattttaac cagctcgctt tcaggagcaa gcaccataaa agttacacca
     6721 aaaattgtat caggtcgagt ggtaaataca tcgaaactta cattttttcc tttaatggta
     6781 aaacgaagtt cggctccttc gcttcttcca atccagtttc gttgagtttc tttcaatgat
     6841 tcagaccaat caatttgttc caaaccatcg agcaaacgtt gagcatacgc tgaaacgcgt
     6901 aaacaccatt gccgcatcac gcgttgttcc actggataac caccgcgaat ggaaacacct
     6961 tcgcttactt catcattggc taaaacagtt cctaacgctg gacaccagtt tactttcgta
     7021 tcggcaaggt aagcaatgcg ataattcaac agaatttcct gttgagcttt ttcactcatt
     7081 gctttccatt catcagcagt aaaattcaat tctattgtac caacagcagt tagattttca
     7141 gtaccatatt tttcaaaatg ttcaatcaac aaagtgatgg gttgggcttt ttgtaatttt
     7201 tcgtcgtaat aatgtttaaa catttgaata aaagcccatt gtgtccattt gtaataatct
     7261 ggatcacaag tacgaatttc gcgactccaa tcgtagcaaa aaccgatttt attgagttgt
     7321 tcccgataac gttcaatatt tttcttcgtt gttatagcag gatgctgtcc cgtctgaatg
     7381 gcatactgtt ctgctggtaa accaaaagca tcaaatccca tgggatgaag tacattaaat
     7441 cccttcatcc ttttataacg actataaata tcagaagcaa tataaccgag tggatgtcct
     7501 acatgaagac ctgctcccga aggataaggg aacatatcca atacataata tttgggttta
     7561 gttaagtcta tttcggtctt gtaaatgtta ttttcgttcc agtaattttg ccactttacc
     7621 tcgatttcct taaaattata ttccataata aattgaaagt tattaattta agttttttcc
     7681 tcctaaattt ttacatttac attagtataa atttgcaaag ttaattaatt tcaatcaata
     7741 aatgaaggag gattctttta gtagaattaa ataacgtaaa gaagagataa ataaaaaaaa
     7801 tgattatttt tgtgagataa atgtttataa tgccattgaa gtgaccaata acaaaccaga
     7861 tatggatttt aaagtccctg aacctacttt gcgtcgtttg ccgtggtatt tagcttatgc
     7921 aaaattgatg cttgaaaaag gagaaacttt tttatcttct acgcaaattg ccaatgatat
     7981 tgctattgac gcttccattg tggcaaagga tttgtcattt gtcaagattt caggtcgtac
     8041 ccgtgtaggt tatgatatca aggaacttat ttatgtgctg gaacgttttt taggatttaa
     8101 tgtttctcat aaagctttcc tgtttggagt agggaatttg ggtggagcct tgatgcatga
     8161 taacggttta gaacaatttg gtttaaaaat tgttgcaggt tttgatgtaa aatatgaatt
     8221 aataggcacg agcattaata aaattcctat tcatcatatt gatcattttc cagaattgca
     8281 aaaacaaaca ggagtgatta ttggaatttt aaccgttcct gtcgataagg ctcaaaaaat
     8341 ggctgaatta atagttgatg gcggcataaa agctatctgg aattttactc cttttcgcat
     8401 acgggttcct gaaaatgtgg tggtacaaaa tacttctatt tatgctcatt tggcagtcat
     8461 gtttaacaga ttgaatgcca ttttggggaa ataaaattta gagataagaa attaaaatga
     8521 aaataatttg tattggagaa aattatcctg aacacaataa ggagcttaat actactaatc
     8581 caggagagcc agttgttttc ataaaacctg atacagcagt gctgaaaaac aacaagcctt
     8641 tttatattcc agattttacc aacgagttgc attatgaaac ggaattgatt gtaaaaatca
     8701 atcgtttagg taaaaatatt gcggcgaaat ttgcctctcg ttattacaat gaaattgggt
     8761 taggagtaga ttttactgct cgtgatttac aacgaaaatt aagagccgaa ggaaaaccct
     8821 gggaaatttg caaggctttt gataatgcgg ctgttattgg taattttttg cctatcagtt
     8881 attttaaaaa tttacaggat atttcttttc atttgaatat aaatgagaaa actgttcaaa
     8941 gaggcaattc aaaagatatg attttttcca ttgatgaatt gattgcttat gtgagtcggt
     9001 tttttacctt aaaaattggg gatattttat ttacgggtac ccctgcggga gtggggaaag
     9061 tttctgtagg agaccatttg gaaggttata tttttgagga aaaaatgttt gatttttttg
     9121 ttaaataatt tcaaaattca gttcaaataa taaataattt tttttagcgt ttcatatctg
     9181 ttaactttgt attgtgaggt aattcatttt atgagaataa aacattttct gtttctgttt
     9241 tttcccttgc tactttttgt tggaaaaagt gaagctcaaa caactttttc atttgctcaa
     9301 aaaatcaact ggataggtgt tcaaaaatgg tttgctgatt caagctatat caatgttatt
     9361 tcttttgaaa atgcttgcta tccttctgat aattttctgc catattttaa ctataaacag
     9421 gaaatggaac cttccttttc ctacgaagct tccataacaa atccagtatg gattcctgtg
     9481 gcgccggatg aactaaaaat cgtagaacaa tattcttttg ttgctgagcc agaaataaaa
     9541 tcgaatgtag ctattgaaaa aggagtttct ttttttagcg taaatatatt gccttttgtt
     9601 gaacgggaag gtcagtattt taaattgtta agtttcgatc tggtgatcaa aaaaacaagt
     9661 gctccgcaaa aaactaaggc atccactctt cacacttatg caacttcttc ggtattggct
     9721 caagggaaat ttgtaaaaat taaagttgct aataatggta tttataaaat tacgtatgac
     9781 gatttggtta aaatgggaat aaatcctacc aacgttcgag tttttggtta tggaggtggt
     9841 gttttggaac aaagttttct caaaaataag attgatgatt tgcctgaagt agccatctgg
     9901 atggaaaaag gaaaggatgg cattttcaac accggtgatt atattttatt ctatgcacaa
     9961 ggacctgtga aatggtatta tgatgaggca aaatcaaaat ttgtacatgt aaataatcct
    10021 tattccctat atggatatta ttttattact tctgatgcag gagttggcaa aaaaatagaa
    10081 gaaaaaacaa ttgaagttcc ttcagacgct gtgattcatc agattacaga atttactgat
    10141 tatcaggttt acgaaaagga tcaagttaat ttgtgtagtt ctggaaaaga attttatgga
    10201 gaaattttta atacgcgaac tagttatgaa ttttctttta atttccctaa tattttatat
    10261 accaatacca caaaagttac ggtggacgtt gctgcaactt cgtcagcaaa ttcctccttt
    10321 acaattagtt taaataatac tcaatcgaaa tctttgactg ttcaaaaaat atcaggagat
    10381 cattacgaaa tgggcaaggc tgctacagga tcttatattt tcactcccaa tagtgaaact
    10441 ttaaatttta aaattgatta taataaatct acttctacat caacaggtta cttaaattat
    10501 attgaagtca atgcgcgtcg aagattacaa atgagtggct ctgtaatgca atttcaaaat
    10561 gtagattatc taaataaatc ctccaactat aataattata ttctcagtaa tattaacagt
    10621 aatattcaaa tatgggatat tactgatcca caaaatatta cacgagtgcc atttactatg
    10681 gtgaatgata atacattaca attcactgct ccaggttacg aattgcatac ctatttggct
    10741 attgatccaa ctgctggttc ttcttttagt acgcccgaaa cagtgggtaa ggttgtaaac
    10801 cagaatttgc atgggctcca gcctgttgat atggttatta tcgttcatcc aaatttcaag
    10861 tcacaagcag aaaaattagc ccaaactcat cgtgaaaaag ataatttaag agtggctgta
    10921 gctactaccg aagaagttta caatgaattt tcttccggga caccagatgc tactgcttat
    10981 cgctggataa tgaaaatgtt ttacgacaga gctttgaatg ccaatcgacc tgatgattta
    11041 ccaaaatatt tgttgctttt tggacgtggc tcttacgata atcgaggaat tattactaac
    11101 tcaggcgata atctgatatt gacttatcaa gctgataatt cgcttgttat tacttcttct
    11161 tatgttacag atgattattt tggtttctta gatgataatg aaggtataga cttgacttca
    11221 aatttattag atattggcgt gggacgtttc cccgtttcaa cagaccaaca agctactgat
    11281 atcgtaaata aaacggttaa ttatattcaa aataaaggaa agggaatctg gaaaaatcaa
    11341 ctttgttttc ttgctgatga tggagattat gctttgcaca tgacgcaggc tgatagcata
    11401 gccacttcca tagcacgatt gtatcctggt tatcagatta ataaaattta tttagatgct
    11461 tttgtacagg aagtgaatgc aagcgggcaa tcttatcccg tagcaaaaag tcgtttccag
    11521 aatttattac gttcaggtat gtttttgctt gattttatgg gtcatgctaa ttctggtggc
    11581 tggacaaatg aagcgatact tacaactgct gatataaaaa atttaactaa tcaacatctg
    11641 gctgtatgga tggcagctac atgcgatttt cttcaatttg atatcaaacc aatttctgct
    11701 ggtgaacacg ttgttttaaa tccttcagga ggtggtattg gcctcctttc ggccgctcga
    11761 cctgtatatg catctcaaaa tttttatatt aataagtatt ttgttgaaag tttatttaaa
    11821 aaggaaaatg aaaatcacct gcgcgtagga gatgctataa gaatggctaa aaacaaaatt
    11881 ggtgctgaaa ttaataaact ttgttacgta tatgtaggtg atcctgctgt aatgttaaat
    11941 tatccagata aattttcagt agtaactagt aaaataaatg gaaataccca gttaggaaac
    12001 gatacattga aagctttgtc tgtaacaaca attgaaggct atattagtga taatgttgga
    12061 aataaaatac ctgattttaa tggaaaaatt gaagtagttg tttatgataa attacaaaaa
    12121 atcactactt tgaacaatga aaaagatgga gctcttactt attatgatcg tccaaacact
    12181 ttgtttgctg gaaaaacaga tgtgaaaaat ggagaattct cctttacttt catgcttccc
    12241 aaagacatca aatataatta tggaaccgga agaattaatt attatgctta tgatgatgac
    12301 catgattatg aagcacaggg ctattttgaa aatttccttg taggaggcag ttcacaaagt
    12361 tatgtaaatg atacaattgg tcccaaatta acaatttatt tgaattcccc cgaatttgtt
    12421 tcaggcggaa aagtaaatga aactccttta tttgtagctc atctggaaga tgaacatggc
    12481 atcaatgcag taggaagtgg cattgggcat gatttggtat taattattga tgcaaatcct
    12541 ctaacttctt atattttgaa tgattattat caatcagaag aaaatagtta tacgagtgga
    12601 agcgtttctt ataaacttcc tgaactgaaa gaaggaaaac atactttaac tttcagagcg
    12661 tgggatttat taaataattc ttctacgcaa acgcttgact ttgaagtagt aaaaggctta
    12721 aaacctgtga ttttctcggt ttacacttat ccaaatcctg ttcagcaaag aacaaagatc
    12781 gttatcaatc atgatcgacc agaaacgcaa ttaattacaa cggtagaaat ttttgatatt
    12841 actggacgaa aaatttggtc attctctcaa tcttctattg acgatattac ctgggactta
    12901 aaaggtgccg atgacaaacg agtacaaaat ggtatttatt tatatcgtgt tagcattaaa
    12961 acacccaata gcgaaatgac ttcgaagaca aataaaatca ttgttgttgg acaataaaaa
    13021 ttaaaaatca cgtttgtata aaatacattt tttaaaataa tctattagta actaaataaa
    13081 taagaacgaa tgaaaaaacg atgttcaatt ttgagtgttt gtttcttttt agctttttat
    13141 ggtttttctc agaaaaataa tcctgttatt acagcagtac cttcattaac tattgctcct
    13201 gatgctcgag ctggtgcaat gggtgacgta ggagctgcta cgcaagtcga tgtaaattcc
    13261 cagtattgga atccagccaa gtatgctttt atggaaagta gtgcaggagt tgctttctca
    13321 tatactccct ggcttagcaa attagtcagt gatattaatt tatactattt agctggtttt
    13381 tggaaattcg atcctcttca atcggtaagt gcatcccttc gttatttttc tttaggtgat
    13441 attgatataa tggatgaatt tggttatcct caaggtgctg ctcatcctaa tgagtttgca
    13501 ttcgatattg cctattcacg tttgctttca actaaatttt caggttcagt ggcttttcgt
    13561 tatattcggt cagatttgaa tagtggagta aatttgagtg gaggtacaga aatgtatcca
    13621 ggcaatgctt ttgctgcgga tattgctggt tattttaaaa cacctatata tatgtcaagc
    13681 ggagacggga atttagcttt tggttttaat atttctaata ttggatctaa aatctcttat
    13741 gatgaaaagg aaaccagtta ttttattcct accaaccttc gtttgggagg ctcatttgaa
    13801 tatccgattg atgactataa taaaattagt tttaatgcgg atataaataa attacttgtt
    13861 cctacacaag acaccatgtc tatagctgag tatagtgata tttcatcaat cagtggtatt
    13921 ttcaggtcat ttagtgatgc accaggtggt ttttctgaag aattgcaaga agtaatgtgg
    13981 tcagtgggtg ctgaatatgt atataacaag caattttcgg ttcgtgcagg ttattttcat
    14041 gaaagtcaaa ataaaggcaa tcggaaatat tttaccgtag gagctggttt taaattaaat
    14101 gtatttgaac ttgatgcagg ttatgttatt gctaccaatc aatccaatcc tttggatcaa
    14161 acactgcgat ttacgttagc atttgacata aatggcttga aaaatttaat aaaataaatt
    14221 tagatttttg tatttatctt tgcggttgtt cccatattgt ccaattctgt ggttgaacaa
    14281 ccgtttttta taattgattg ataatgaact ttcgaatagg ttttggttat gacgtccatc
    14341 catttaagga agagcgagaa ttatggctgg gaggaattaa gattgattat ccttatggtt
    14401 tggcaggtca ttctgatgcc gatgcactta ttcatgctat ttgcgatgct ttgttgggtg
    14461 cagcttgttt aggtaatatt ggaattcatt ttcctgatac ttctgatgaa tttaaaaata
    14521 ttgacagcaa aatcttattg gatagaacat tacaattaat cagaaaaaat ggctatgaaa
    14581 ttggaaatat tgattgcacc gtttgtgcag aagaaccaaa acttaatcct tatattccag
    14641 ccatgcaaaa atgtttatcc aaaattatga atattaaaat agatgatatt tccattaaag
    14701 caactacttc tgaaaaaatg ggttttgttg ggcgaaaaga aggtattgca gtgtatgcag
    14761 ttacactgat ttataaaaaa taaatattga tgaaagtata gtattaatat tttccagaaa
    14821 caacttccca attgatcaat tcccagagag cagccacata atcggcacga cgattctgat
    14881 aatcaagata ataagcgtgt tcccaaacat caaatgttaa aattggcgtc aatccttgta
    14941 cgataggatt acctgcattg ctttcattga caattgcgag tttgccatcc ttgtccttta
    15001 ccagccaagt ccaaccagaa ccaaaaagag ctattgctaa tttattaaat tctgtcttaa
    15061 aattatccag tgaaccccat cctgattcaa ttgctttcaa caatttatca gagggtttgg
    15121 tacctttatc aggagttaaa gctaaaaaat aaaaattatg attccagact tgtgcagcat
    15181 tgttaaagag tgcaccactg gtttttttaa taatagtctc cagatcagca ttttcaaatt
    15241 ctgttgccac tattaaatta ttggtattat ttacataagt ttgatgatgt ttaccatagt
    15301 ggaaactaat agttttttca cttatcacag gctctaatgc attataagca tacggtaatt
    15361 ttggtaattc aaatttcata gcaataattt ttttaagatg agtattaata attttctttt
    15421 atatttttta taacacgtaa aaattcaatt tgttttattt cttaaaaaaa tcctatcaaa
    15481 gatgataaaa aatcaggtga cttaataaaa aaggacttca aaacttaccc gctaaaaaat
    15541 tttcacttat ttatctgttt ttagtaataa aattatagta tatttgttgt tctgatataa
    15601 tttaaaagtt ttttatacct ttatctctat aaaaatttct tttaaaaaat actatggatt
    15661 cggaaataaa attagtgatt agtgggttag tttacaatca gaatattgtt ggcacctatg
    15721 gtttgatatt aagtgaagaa tcgggcaatc gaagattttc ggtaatgata ggtgagccag
    15781 aagcccaatc tattgcttta aaactgaata ataaaaaatt accacgtccc ttaacacatg
    15841 atttaatcaa aaatatttta gatgcttttg aagttacatt agtaaaagtt cttatcagcg
    15901 acatgatcaa tgatgttttt tattcagaat tacatatcaa gaaggacaat aaaattctgg
    15961 tagttgattc tcgtacttct gatgccatcg cattggcggt aagatgcaat tgcccaatat
    16021 atatcaaatc tgaaatttta gatatggtag gagtagtaat agaaccaaca aatgaagaag
    16081 aagaaactat ccctgaaaaa actgagtatg ataaaatatc agattcacaa ttagatcgac
    16141 tttctctgga agaactagat gaattacttt ctatggcagt aaatgaagaa aaatatgaat
    16201 tggcggtgca tattcgagat gccataaatc gtaaaaaatc aagcaagcaa taacagcttt
    16261 cctttttgtt tgctcaattt tgaaatagaa aaaatttcca aattttcata aaagatattt
    16321 ttaatctttt ctgaatcaac aagatattta aaataaaaat tttaatcatc atgagtttaa
    16381 agtttcgtct tgttatatta agttttcttc aatattttat ctggggttcg tggcttattt
    16441 cattgggaag ttatttagga aatgtattgg gatttgatgg cattcagatc ggaagtattt
    16501 ttggggctat gggaattgtt tctcttttta tgcctggttt gttaggaatt gtagccgacc
    16561 gctggatacc tgctcaaaaa ttattaggaa tctgtcatct tttaggagct atttttctta
    16621 ttctggcttc ttttcaaaga gaatattttc ctttttatat tttaatgttc ctcaatgtaa
    16681 tgttttatat gccaactatt tcgttaagta atgctgtagc ttataacgct ttagaagaag
    16741 ctcattacga catagtaaaa gattttcctc ctattcgtgt atggggaacg attggtttta
    16801 tttgtgcaat gtggacggtt gatcttacgg gcttcaaata ttcacaagtt caactttatg
    16861 tagcagcaac tgcagcttta ctgttgggaa tttattcttt tactttacct ccttgtaaaa
    16921 tcaataaaac acaagaaaaa aagtcgcttg ttgatgcttt aggattgcgt gctttttcac
    16981 tttttaagca aaaaacaatg gcgattttct ttatcttttc tatgttattg ggagcttcac
    17041 tgcaaattac aaatggtttt ggcgatttgt ttctcagaag ttttgaaaaa atgccagaat
    17101 atgtcaattc ctttggggta aagcattctg tgattttact ctccatttca caaatgtccg
    17161 aaacattgtt tattctggct attccttttt tcctgaaaaa atttggaata aaaaaagtga
    17221 tgttaatcag tatgatagca tgggtttttc gttttggact ttttggattg gggaatccag
    17281 gagagagatt atggatgcta attctttcca tgattgtcta tggaatggct tttgattttt
    17341 tcaacatttc aggttcattg tttgttgagc aatctactga ttcttctatt cgtgcttctg
    17401 ctcaaggatt atttatgatt atgacaaatg gtttgggagc aacaatggnn nnnnnnnnnn
    17461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ttctgctcaa ggattattta tgattatgac
    17581 aaatggtttg ggagcaacaa tgggttctta cctggctgga atggttgttg ataagtttgt
    17641 cactcatgcg gctgttccaa gttggtcaac agcatggttt attttttcag gttatgcttt
    17701 agttgtggca attttatttg caatattatt caaatacaaa tacgagcctg aaaagtcacc
    17761 agagatttca cattaaaaaa taaaagtgtt tttgaatttg aatgagaatg aaaaaacctt
    17821 tttagaaagg aacttcttca ctattgtcat ataaaaacga gtcaagagag gaaaaacgag
    17881 aagcagtgct tttttcagtt ttatttattt ttgaaggaat tactttgtat tcattcaaaa
    17941 tttcctcctg agcatcaaaa tcatccagat ttaaaaattt tgcaaattca cttttaaatt
    18001 tcagttgaat catgtctgtt gcaccatttc tatgcttggc aataataatt tcagcaattc
    18061 ccaaaaggga gtttccatct ctatcttccg tgatatggta atattcagga cgatggataa
    18121 agcaaaccac atcggcatcc tgttcaatgg cacccgattc ccgtaaatca gctaattgag
    18181 gtcgttttcc ttccaaaccg ctacgacctt ccaaactacg attgagttgc gacaaagcaa
    18241 tgataggaat atccaactct ttagccagtc cttttagtga tcttgaaata gtactcactt
    18301 cttgttctcg gctgccaaaa ttcattccgc tggcattcat taattgcaaa taatcgataa
    18361 tgatacattg aacatggtgt tctttcacca gacgacgtgc tttacttctc aattcaaaga
    18421 tggataaact gggcgtatca tcaatataaa tgggagcatc aattaaatct ttaagatttt
    18481 tatccaattt ctcccattca taaggctcga gttctccatt tttgattttt gatccatcta
    18541 tttcacaaat attcataata agccgattgg ctaattgaat attcgacatt tccagagaaa
    18601 aaattcccac tggaatatta taattcattg ccatattttt tgccatcgaa agaacaaatg
    18661 cggtttttcc catcgaaggt cgcgctgcaa taatgattaa atctgatttt tgccaacccg
    18721 aagtaatttt atctaatgaa tggaaacctg tgggaatgcc gcttgcacct tcgcgactgg
    18781 cagcagcctg aatacgttga atagcttcgt ctaatacaga actaattgga agaacttctt
    18841 tttttaagtt acgttgcgaa acttcaaata acatcgcctc cgattcttcc aataaatcat
    18901 ctacatcggt cttttcgtca aaagcttttg tctgtatctc gtttgaaatc cttattaact
    18961 ctctggcaag atatttttga acaataatcc ttgaatgata ttccaaatga gctgctgaag
    19021 aaaccttagc cgtaagtaaa gtaatataat aaggacctcc tacttcttcc aacgaaccat
    19081 tttttctaag ttgctcggta acagtatgca tatctattgg ctcctgatga taagccaatg
    19141 taacaatggc ttcaaaaatt ttttgatgag cggttttgta aaaatattca ggttttaaaa
    19201 tttctgaaac ctgaaaataa gcatcttttt caatcatcaa agctcctaat acggcttctt
    19261 ccagttctgg agcttgagga ggcatttttc cgtattcgtt gggagaaatt tgtaccgtcg
    19321 aaggtgttgt agcctttata tattttgatt tttcagccat ttgtcagaat aagagacaaa
    19381 aatgatttta taaacttctt tccaaaaata caattttttt ctcctttcgt aaaatatttt
    19441 taaaaataaa aatgttaaag tttttttgaa aaggcaaaag acaatgttta ttgtagttaa
    19501 ttttaagaag ctatattttt tggcagaatc atgttgtttc attgcctaaa ttatctatct
    19561 ttgctctatg gttaactgtt ttatttttgc aactttttga ggaaaaaact taaattaatc
    19621 agcgatttct ggaaagcaag gaaaagtttt ttctttattt tgaaaaaata tttagcactt
    19681 taattcttat tttaaagtta atcatttgat aattaattaa ttatttattt tatggaagac
    19741 gaaaaaaatc aaatcagcat tgaactttca gccgaagtag cagaaggtat ttattctaac
    19801 ttagctatta tctcgcattc ctcttcggag tttgtcattg attttatacg aattatgcca
    19861 ggtattccca aagctcctgt aaaatcgcga atcattctta ctcctgagca tgccaaaagg
    19921 cttttgtttg cgttagaaga taatattgct aaatacgaac agcaaaatgg aaagataaaa
    19981 attgctgaaa atacttttat tcctcctttt cctatgggat ttggtggagg agaagcatga
    20041 ttgaaattaa tagtaattgg tatcaaggaa taattatttt tttggtttat tttaaggagg
    20101 gaggattttt ttctctttca agtataaatc tccatggctt gtttatccat tcttcgccgg
    20161 catagtttat tcctatgcgt ggagctgatt tgaatacaat tttctcattg gtattttcaa
    20221 tccaaattct ttgagaggta atgagatttt ctccataaaa acttctgtcg agttttagtt
    20281 cgcgtcctac tttccctgaa ccgattatgt catttaaacc tcgaatcaat actgcttctg
    20341 gatgattttc tggacctgtc accacattga gcatccagta cattccataa ataaagtaaa
    20401 cataaacatg tccacctgaa gcatacataa tttctgttcg tggggttctt cctttgctgg
    20461 catgacaggc tttgtcttct tcaccaaaat aggcttccgt ctcagtgatg atatatcgtg
    20521 aaaaagaacc atcttcccat cgacgaatta gaattttacc taataaattt tcggctactg
    20581 taacagcatc cttagcatag aatgtaaaat ctaatcgaga cataattata taaatcgaaa
    20641 aaataaaaga ttcattaaaa aactcctaca aaaggcaaaa ttaatatttt tctttctctt
    20701 ttttaaagaa aatatttgat aattgttttg tcaatccaaa gaaaaacagt atttttgcaa
    20761 tccatttatt tctttcttcg aatgagaata gaaagttgat tttttatttt aagtgattca
    20821 tttttagttg aagaaagaaa ggaaattaat taaataagga aaccgaatta aattactaaa
    20881 aaaattttat aaagtggata ccttaagtta taaaaccatt tcagccaata aagcaactgt
    20941 acaaaaggaa tgggttttaa ttgatgctac agatatggtg ttaggcagaa tgggttctaa
    21001 agttgcaaaa cttttaagag ggaaatataa acctaatttt acgcctcatg tagattgtgg
    21061 tgataatgtg attattatca atgcgagaaa ggtaaaatta actggagata aatggacaga
    21121 tcgagtgtat ttacgatata ccggatatcc aagcggtcag cgtgaaacta cgccagcaaa
    21181 gattatggaa aaggatccag aaaaattaat ccgaagagtc gtgaagggaa tgttgcccaa
    21241 aaatcgttta ggagcacaat tgctgaaaaa tttgtacatt ttccctgacg aagagcataa
    21301 tatgcaggca caaaaaccaa aacaaattga tttaaactca cttaaataat aagtatggaa
    21361 gtaataaatg ctttaggtcg tagaaaaaca gcagttgccc gtatttttgt tagcgaagga
    21421 aacggaaaga ttactattaa taagcaggat ctttcacagt attttccttc tccactttta
    21481 caatatattg taaagcaacc gcttaataaa ttaaatgtag ctgagaaata tgatattaaa
    21541 gtaaatctca atggcggtgg ttttaaaggg caggcagaag cattacgttt ggcaattgct
    21601 cgttcattag taaaaattaa tcctgaagat aagaaacttt taaaatcaga aggcttttta
    21661 actcgtgatc cacgtattgt agagcgtaaa aaacctggac gtcccaaagc acggaagaga
    21721 tttcaatttt caaaacgtta atttcaaatt ttatatctat tctttttgca tgatattttt
    21781 tacagaaaag ttttcatgca aaggaatcaa aaagtagttt agtatctaaa ttggcgagac
    21841 tcttttatga aaaaaggcta ctcgttgatt gattttaata gaaggtaaac aattaaaaac
    21901 aaaaaaatgt caagaacaaa ttttgaagaa ttattagaag caggttgcca ttttggtcac
    21961 ttaaaaagaa aatggaatcc agcaatggct ccttatattt ttatggagcg aaatggtatt
    22021 cacattattg atttatataa aaccatcgca aaaattgatg aagcagcaga agctttaaag
    22081 caaattgcaa aatctggaag aaaaatttta tttgtcgcca caaaaaaaca agctaaagaa
    22141 attgttgcgg aaaaggcaaa agctatagga atgccttata ttactgagcg ttggccagga
    22201 ggaatgttaa ctaacttccc gactatacgt aaagccatta aaaaaatggc tactatcgat
    22261 aagatgatga ccgatggaac atttgataat atttcaaaac gtgaaaaatt acaaattact
    22321 cgtgaacgag aaaaattaga aaaaaatctg ggttcgatag ctgatttaac acgacttcct
    22381 gctgccattt ttgtagtgga tgtaatgaag gaacacattg ctgtaaagga agcccagcga
    22441 ttagaaattc ccatttttgc aattgttgat actaattctg atccaacagg tattgattac
    22501 gttattccag ccaatgacga tgcaacaaaa tctattgagg tgattttaaa tgcattggtt
    22561 gcagcaatac aagaaggtct ggatgaacgt aaattgatga aagttgaagc tgaagcttcg
    22621 gaagcgccag ttgtgaaaga aaagaaatca aggattaatc gacgaccaag aactcaaaag
    22681 gaagatgaag aagctttgaa ggcaaatgta ttaaataaat ttatcaaaga cgaagaagaa
    22741 taagcttaat gataatttaa acaaatcctc tttaaacgag ttacaggtta tcattatttc
    22801 atagctttgt gactcgtttt taaattaaat aaccatttat ttttttaatt agtattttag
    22861 aaaggagaat attgagaact atggcagtaa caatgaatga tattacaaaa ttgcgtgcta
    22921 tgacaggtgc tggtttaatg gattgcaaac aagcacttat tgaggcaaat aatgattttg
    22981 aaaaagctat tgaaatcgtt agaaaaaaag gacaagccat tgcagccaag cgtagcagtc
    23041 gtgaagcatt agagggttgt gtattggcag aggcaaagga tgattttgca gttgctttgt
    23101 ctttaaaatg tgaaactgat tttgttgcaa gaaatgctga ttttatagcg ctaacaaatt
    23161 caatattgca agcagcttta gaaaatagac ctactactaa agaagaactt ttagctttaa
    23221 aaattaatgg acgaaccata gccgatttgg ttatagaacg ttcagctgtt acaggagaga
    23281 aagttgaatt agaagattat gttttcgtta aaggtggttc caccgcagca tatactcatt
    23341 ttggaaatcg cttagcttcc attgtagcct ttaaagaaaa agatgttgat cccaaagttg
    23401 ctcatgaaat cgccatgcaa attgcttcaa tgaatcctat tgctattgat cgtgcctcag
    23461 tgcctcaaaa aatgatcgag acagaactgg aaattgctag ggaaaaagct cgtgaggaag
    23521 ggaaacctga aaatatgatt gataaaatag ctgaaggtcg cttaaataaa tttttccaag
    23581 aagctgtttt actcgaacaa acttctatca tggacaataa aacgattatt ggcaattatc
    23641 tgaaagctca caatgcaaca gtgattgatt ttaaacgcat aagtctaaat caggaataaa
    23701 tatctatttt cccctctttt taggaaaaac cgctggattt tatcagcggt tttttattac
    23761 acatctcata ttacaatacc tgataataaa attccatcac tacaaataat aacgaaaaat
    23821 aattcaaaaa gaattaggaa ttccgacttt tgaatggaga cattttgtga aatatctgaa
    23881 tatgcaaaca aaaaatccct gtggttctca gcgtaataaa ctattcattt ttttaatttg
    23941 ctataatgaa ttggaaagta atttttatat ttccccg
//
LOCUS       DAOSAS010000006        23261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_1586_length_23261_cov_10.331380, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000006 DAOSAS010000000
VERSION     DAOSAS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 23261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23261
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1586_length_23261_cov_10.331380"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLR72_00635"
     CDS             complement(<1..347)
                     /locus_tag="PLR72_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK35752.1"
                     /translation="MSAAFLFSSSSCIKDNVIKTDVLIIGGGTSGISAGIQSSRLNAK
                     TVIIEESTWLGGMLTAAGVSAVDGNYKLPSGMWGEFKDSLTNYYGTPEALKTGWVSNV
                     LFEPSVGDKIFKN"
     gene            complement(414..>1096)
                     /locus_tag="PLR72_00640"
     CDS             complement(414..>1096)
                     /locus_tag="PLR72_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase family protein"
                     /protein_id="HOK35753.1"
                     /translation="YIGQKIENFPNDIYEWQKDSKGNFYPKEGKHFLKWIEWRAKNIY
                     DFMSLAKEKVKSVNKNISFGVYTGAWYPSYYEVGVNFASKNYDPSKEYKWATSEYKNF
                     GFRELLDLFTVGNYYSTISIEDYLKNHPMIKNETDFKFQQGTWYCVEGSCKKLKKILD
                     GNSFYGGILASQFYNQRKDLTRSIIMNLQESDGVMIFDISHIIEKNLWNDVQNGMLES
                     NPLETIKK"
     assembly_gap    1097..1196
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1197..2017)
                     /locus_tag="PLR72_00645"
     CDS             complement(<1197..2017)
                     /locus_tag="PLR72_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35754.1"
                     /translation="MAKKTSCKFFYVLSIAILLFSCNNNENGITITPDMFPPQNDTIQ
                     EPNDSTEIDSIEENIDFQNFLSNEIPPGATPNCFLGAYYRKILSSNDHWLGICGTVIL
                     PTISFDPQRINPNKPAQYLDNPSIYLGGRADGQETDIGLTWEVIMMETGGISADRRAF
                     RPFYRWASHASSGQKSGYGNAPVQEKHYFYPGDTVFVSVQIIHDRTLRFIVEGKGKKF
                     QTDINCNGYTLDGKGEYKRVNAIDQVGNEGKPVQPTNTKVIGAKWLETYLYRNVEG"
     gene            complement(2031..4022)
                     /locus_tag="PLR72_00650"
     CDS             complement(2031..4022)
                     /locus_tag="PLR72_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOK35755.1"
                     /translation="MKLKPIFLSFLLTFIFLSFPLTSQISIVAHRGASAYELENSIAA
                     FKKAFELNADAIELDVWKSKDDSLIVMHDQMTGRTANKNLNISTSTAKELRDLQLKNG
                     EQIPFLQEVLNILPEGKKVVIEIKDENEKNTKKIISNLNSILKRSGKEKDAVIISFHL
                     PMLIEAKKQLPHLKCYYLSSEKNKENELIAICKKYNFDGLDVHYKILTENLVKKAKEA
                     GLDVWTWTVDDPKIALFTQVNNKVSAITTNCPDVISDILEKNNYQTIYYEQKRSIYKI
                     LPRENNEIIFLGNSITDYGEWPEFFQNSKIKNRGISGDITLGVLARLNDMLVAQPSKI
                     FLLIGINDISKNNPDSIILYNYQMIIEEILEKSPTTQLYIQSILPINNDFKKYPRLQN
                     KEKHILFLNKKLKELCQEENLTYIDLFSVFCNEKEQLKPEFTNDGLHLTGEGYKMWIE
                     ILKPYVNENKPATNKLFESPYYMNRLKAHRQEKTRANSIVFLGNSITEQGWWSILLKN
                     EDIVNRGIGGDNTYGILHRLPEILQTKPKKIFLMAGINDITAGRDITEIILNFKKIIE
                     ITQTVSPHTQLYIQSVLPVNDTSLAYNELKGKNSTIIQLNSQLKKLCEEKNIPYIDIA
                     SLLSDEKGQLKANLTKDGIHLQPEAYIIWTDYLKKMNYL"
     gene            complement(4043..5377)
                     /locus_tag="PLR72_00655"
     CDS             complement(4043..5377)
                     /locus_tag="PLR72_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase family protein"
                     /protein_id="HOK35756.1"
                     /translation="MKKHCGFCLILFLIIQFFSCKDSEHPLPKWEWNDNYEFLPVEKK
                     PKILWIDASANFKYFANSQENIQFYLEKAKNTGFTEIIVDVRGTSGDVLYQSNIVDPL
                     TKVGDVERTATWDYLTAFIEKGHALGLKVHANINTFVGGNTSNSGTGIVYREPDKIEW
                     LTILYLPGAQGITNIMYDSNSTTKFFNPVNENVQNYILSIIAELATNYPTLDGIVLDR
                     CRFNSIESDFSNLTREKFETYIGKKLTNYPDDIFKWDSTPNTDGTYNRIEGLYFKLWL
                     EFRAKTIHDFVERARNTIKTINKNIQLGIYTGSWYSSYYNEGVNWASPKYDPSQKYKW
                     ATPNYKNYGIADLLDFYMTGAYSKTLYGSGEWTVQGALQQAQEVTKGDVLVCGGLYGL
                     NFYNAPQDCEKAVYICLTESNGLMFFDIIYLIMYDQWENVKAGINKALTNND"
     gene            5431..5544
                     /locus_tag="PLR72_00660"
                     /pseudo
     CDS             5431..5544
                     /locus_tag="PLR72_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_091910270.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(5606..7297)
                     /locus_tag="PLR72_00665"
     CDS             complement(5606..7297)
                     /locus_tag="PLR72_00665"
                     /inference="COORDINATES: protein motif:HMM:NF027725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5018 domain-containing protein"
                     /protein_id="HOK35757.1"
                     /translation="MKKYIFLFITSLTLMCLFHSCEEPEEIQRIDTESNSGIMQLVCT
                     FMDGTGEFTIDTTINKYPFHDGDTVKIIVPWFYPPESTNETSIDKMKIKASLPNNVYV
                     SPPFDIIDLTKVTPYTVTSPSGRKITIYITGVRKKSSEAMIKEFELPAVGLKGFILEE
                     QNFIGIVSGGMDLSNQKPKITISYHATISPDTSIAQNFNEPVIYTVTAFDGTQRTYTV
                     SPFTPQKVAYGIRPASARQLWFKSLGEMGLPPADHYSTAIAVSGNYLVINTRNQDNVY
                     VDRFSGEPKGRMQLGPIKGSLTNFFATSDSVGHILICNLVPNAGPNFKVYKFNNVNDA
                     NPVLLIDWPTGGIAMGRKMSVRGNLDGDALITVTQMESKIVHIWEVKNGSIQSAPDGG
                     PTRSISVAFENNWTFQSDAIARSATYPSILFISGYPSSFGAVNADGSVLALYDLIGAG
                     YDSNFVPQSLDYAIFNGAHYLAQTIVALYGGGNNCHLYDVTIPTNLSLAPKDPRLLVF
                     SSPPNVVTGNTNATGDVAIKVSDDGYKMIMYLLITNFGVGAYEFDCIDVDNIFGE"
     gene            complement(7310..8944)
                     /locus_tag="PLR72_00670"
     CDS             complement(7310..8944)
                     /locus_tag="PLR72_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOK35758.1"
                     /translation="MKKKIFKLTILSFMALLTSCNDWDYLDPVPTDRYSDKMVWQSEK
                     NADLYLNGFYTYISTYGNFGNGQFGGLLTEGLTDMLKYGSNVAGDGTPNDYAVEPSKI
                     TPDQNLLGVWGDAYNRIRRVNEFLVGLKEYANFSNEIKNRYEAQARFFRAYLYHQLVI
                     RHGTAILLDAPTTNKNNPLSSEEECWNFIENDLDFASRYLPIQWDDANKGRLTKGAAF
                     AFKSRAMLFARRWEKAKAAADSVFALNIYELAPTYEEAFKSYMKTGNKESILEFNYAL
                     PAPSHNFDRQYAPGGDEAEMGGKATPTQEMVEEYELATGGKPDWSTWHSTSGTTETPP
                     YEQLEPRFHASILYNEANWKGRKIEPYVGGKDGFIQYGEEQYPKGKTTTGYYLKKWLD
                     ENNTDLVNIRSTQPWIEIRLAEVYLNHAEACAMLGENNSANQDLATIRARVGLPHTTK
                     TGNDLIEAIRHERKIELAFEGKYYWDLRRWRIAHQVLTGVRFHGLRIEKASNQFTYKY
                     VECDDRDRQFPEKLYRLPIPSNEIANNLAISQIEGW"
     gene            complement(8973..12116)
                     /locus_tag="PLR72_00675"
     CDS             complement(8973..12116)
                     /locus_tag="PLR72_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK35759.1"
                     /translation="MKNKNLSKLEFRGLKVFVLLGMLSCALSMSAQINIQGKVIDQNN
                     EPLTGVTVMIKGTSIGTITDINGNFSLSVPDQKAVLVFSFVGYQKQEVPVGTSRQLTI
                     TMIEEPKLLQEVVVVGYGTQKKVSVTGSISQISGAEMLKAPPVNLSQMLGGRVTGLVT
                     RQSSGIPGSDDASMVIRGITTTSGATDPMIIIDGVPRSFNQLDPNEIESISILKDAAA
                     AAVYGIQGANGVILVTTKKGMEMKPQITYSGNFEINQNTSFPKFMNGPQYAYYWNKAL
                     EMDEQAPVFTEEMVNKMINGDPEGVYGNTNWVDEIFKTGQTQHHNLSATGGTKDVKYY
                     MMIGYYDQKGNVKNFDFNRYNVRSNIDANIVKGLTANFNIGARKENRNRPYFGAGKNE
                     YMSIIQQAIRAHPYVPLTYNGLPTATKTASAQVSPVAARDLSGFNDSEMSVFQSNFAL
                     NWEIPWLKGLSARAMGSFDRDYTHSKVFKTPFQVSLASRPTPTYPSLKYETMYPVAIG
                     NVATMSEGFAEATRITFQGQINYAQTFGKHDISALALWEQSNYIYKQFNVNVKDFDFY
                     DFAELNHAKSIADQNQFSGSSKTSPRAGFVFRASYAYEQKYLLEVSGRYDGSYRFSPE
                     TRWSLFPAASIGWRISEENFFKEAIPFVTNLKLRASAGQLGNDAASPEFAYLRTMAWV
                     SEDPSVVIGNIPQKALMTSGVPSNNTWEVTTTYNAGFDLLMWNGLLGMEFDWFYKLTT
                     DILRSQAGIWPPSVGGNFPSVINAGKVDARGFDLVLTHENKINQFNYGARLSLNWARN
                     RILNIDDSPNIPDYLKRNGLQVGMKDGFVSLGLFKSDEEAAIYPTVREGAKGGDIRYK
                     DLNGDGKITYDQDRAWIAKSNIPELMGGLDLHASWKGFDFSILFTGAAFCDIALMGYY
                     EGIGWDNTEFTRPFYHDGNSPLSLVENAWTYENPDAKFPRLSTQTRWNNNWASTFWIV
                     DGSYLRIKNLQIGYFLPQKFVNTIGMKSARVSVSGSNLYTWANKRLNGLDPEAPDVCN
                     GYYPQQKTYSFGINLTF"
     gene            complement(12197..13612)
                     /locus_tag="PLR72_00680"
     CDS             complement(12197..13612)
                     /locus_tag="PLR72_00680"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOK35760.1"
                     /translation="MKRAVALDVLRGISIFGMVLSGNIPFGVLPAWMYHRQTPPPSHV
                     FNPDVPGITWVDLVLPIFIFCMGVAIPLALNKKIEAGKNFWNIAQSIIKRYFMLVIFA
                     ILIAHFQTSAIPKSFVNFHFLGIYIKGYDLFLITIIGFLSLFSIYVIIENQQQKKKWR
                     IIGWTAIVSLLLLLHYVYGLKFSLHRSNIIILLLANVYLFGAFSWYFTRSDKLNRLLL
                     LMIWGAIQITCKTTHFDEALNQISGLSWLFLFRMTHYMLLIIPATIVGDIIYERLTAE
                     NSKNTSHSKKKGEKFISHLIFLLGIWFIVALYNRWLVILYISTPIVLACFYYIVKKYS
                     PSSLSFFKIAAYMILVGLILEPAEGGIKKDPVTASYLFITGGISILWLIFLDYVCQRN
                     EKSHFVKLFAGAGSNPLLAYIATNWFVIPLMNLTFLIIPYQGCYPDGYHWLGVLRAFI
                     LVFCVMFLVALATKKKIRWRA"
     gene            complement(13768..15027)
                     /locus_tag="PLR72_00685"
     CDS             complement(13768..15027)
                     /locus_tag="PLR72_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK35761.1"
                     /translation="MKKITFLMSACLTVAALQAQTEPTPTITTLWEKSAASGSLPAWF
                     LTTTNKTRGIGASNEHVFVATREGTSGKNIFVYNATTGDSICALNTANIVDGLFPLND
                     VGATDDGKIITASLGANTNFKIYMYDNLTAEPVVVLHYPDAAPGGRTGDLFTVVGDYS
                     EGTAKIYTATAANPSDIYVLEMENGQWKTERTLVKSISLDGNAVCPGIGVKPNGDLFW
                     KAGGKKIHLITNEAVLSEPEQTISMYSNSVKYIGYSSTYNLDYIVLFTYGGQKECAEI
                     ISLTPGDLSTCQIIARTPSLGANANVGGTGDVEVRYDQQGNPIIYVVSTNNGFGAYKV
                     EGLDITTGLPMPPEKIEVNIYPNPAKEKVHFSQTAKKVKLFSISGQLLKEANNVDHLN
                     VKELKGIYLLEIESQRGEKVFKRLIVE"
     gene            complement(15251..16420)
                     /locus_tag="PLR72_00690"
     CDS             complement(15251..16420)
                     /locus_tag="PLR72_00690"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOK35762.1"
                     /translation="MNFSKLAKQYQNELLNNVIPFWLNKSQDKEFGGYFTCFDGQGNI
                     FDTDKFIWLQGREVWMFSTLYNKVEKKQEWLDCAIQGAEFLKKYGHDGEFNWYFSLTR
                     EGKPLIEPYNIFSYTFAAMAFGQLSLATGNQEYEQIAKATFDKILSKKENPKGKWNKV
                     HPGTRDLKNFALPMILSNLALEIEHLLDEKFLLQTLENITHEILEVFYRPELGIVVEN
                     VTLTNELSDSFDGRLVNPGHALEAMWFLMDVGERLNRPQIIEKAVEIALKMVNYGWDK
                     KHDGIFYYMDRKGYPPQQLEYDQKLWWVHIEALIAMIKGYQLTGNKECLQWFKKIHAY
                     TWSHFKDPLYPEWFGYLNRRGEVLLNLKGGKWKGCFHVPRGLYQCWKTLEKISDL"
     gene            16884..18092
                     /locus_tag="PLR72_00695"
     CDS             16884..18092
                     /locus_tag="PLR72_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOK35763.1"
                     /translation="MRYKLIDEIELGSKNALLKINILQYYINNGENSLADLAKEMNLS
                     IPTVSKLVNELIEEGYVIDFGKQETNGGRRPSIYGLNPDSGYFVGVDLKRFRINLALI
                     NFKGEMVDSKLSIPFNYENTQESFDTVCKIIDDFVNQLSIPKSKILSVGVNISGRVNT
                     QTGMSYSYYFFKEEPLTEIFQQRLGLHVTIDNDSRAMAYGEFMKGDVKSEKNVLFVNV
                     SWGLGLGIIVDGKLYYGKSGFSGEFGHISAFDNEVLCHCGKKGCLETQASGSYIYRKF
                     IEKVKEGNSSILAGRIKKGESITQEDILEAALNEDMLAIELVEEVGYTLGKHIAGLIN
                     LFNPELVIIGGVVAMTGDYLLLPIKSAVKKYSLNLVNKDTTIKLSKLGDLAGVIGACM
                     LARSKMLELI"
     gene            complement(18304..18678)
                     /locus_tag="PLR72_00700"
     CDS             complement(18304..18678)
                     /locus_tag="PLR72_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK35764.1"
                     /translation="MEFIDSQPIYLQIANYVCEQILLRRWKEEERLPSVRELSALLEV
                     NPNTIMRAYDFLQGNEIIYNKRGIGYFVTKNAIEKIIDYKRKQFVGKELPVFFKNLQL
                     LDISLEEIKKLYEEYNQQQSIQ"
     gene            complement(18678..19325)
                     /locus_tag="PLR72_00705"
     CDS             complement(18678..19325)
                     /locus_tag="PLR72_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35765.1"
                     /translation="MFFKGEGTAQLITLIVGIVYATRFFKIFAYSSNGTHYLMIPATQ
                     VEKLITVFLLNTFYFFLMIIITSVIGNFIGTILRNLIYGISTPFSFQFFFPYSKLNSD
                     FDITYLLKNFGVFATSQAIFTLGSLYFKRNSIAKTLLTLMMISILFGMIEYTVFKVTM
                     GSLLSALNQYDGFQMAWDEIIFPKGMIFVWKIIGYLLIPFCWIVSYFRLTEKEIK"
     gene            complement(19450..20307)
                     /locus_tag="PLR72_00710"
     CDS             complement(19450..20307)
                     /locus_tag="PLR72_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK35766.1"
                     /translation="MIDIKNLEFNYSHSSHLFKNLNLSLFAGHIHGLLGKNGEGKSTL
                     LKMISGLVFPLKGEISVMNVEPRKRHPEMLQEIFFLPEELPPVNLSMENYEKVYAPFY
                     PHFSSEQFHQYLTEFEMESAKTQMNQLSYGQKKKFFIAFGLAANTSLVLLDEPTNGLD
                     IPSKAQFRKMAALAIEENRCIIISTHQVRDLDSLIDSIVIMNGHEIVFNEFTENITKK
                     LLFKVSNQKEEKEEVLYAEENLRGTYQVVENKTGEESRLDIELLFNAVLQNQQRIKEI
                     FSNQPNNFK"
     gene            complement(20704..21753)
                     /locus_tag="PLR72_00715"
     CDS             complement(20704..21753)
                     /locus_tag="PLR72_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HOK35767.1"
                     /translation="MKWDTVKITNQHGEMIEAIAPIIISASRATDIPAFYSDWLIQRI
                     NEGYLKWNNPFNGVSSYVAFSKARFFVFWSKNPKPLLKYLDFFEEKGFNYYFQFTLND
                     YDKEKLEPRVPNVQQRIETFQQLSERIGKEKVIWRFDPLILTDKIGIDELLRKIENIG
                     NQLKNHTQKLVFSFADIKIYKKVQNNLRNSSIHYQEFNERTMNEFAAGLQALNKNWNF
                     ELATCAEQISLEKYGIKHNKCIDDDLIIKLFYQDKKLMDFLAVKIIPADIFHPTPTFV
                     KTKDNKDKGQRKFCGCIVSKDIGEYNTCPHLCEYCYANSSKQIALENWELHKRHPYDE
                     LLFRSIKSPKLSQII"
     gene            complement(22517..22627)
                     /locus_tag="PLR72_00720"
                     /pseudo
     CDS             complement(22517..22627)
                     /locus_tag="PLR72_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203020.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         7525 a         3835 c         3545 g         8256 t
ORIGIN      
        1 atatttttaa aaattttatc acctacagag ggttcaaata aaacattgct tacccatcct
       61 gtttttaatg cttcaggagt tccatagtag tttgttaaag aatctttaaa ttctccccac
      121 atcccacttg gtaatttata attgccatct acggcactta ctccagcagc agttaacatt
      181 cctcctagcc acgtagattc ttcaatgatt actgttttag catttaatct tgaagattga
      241 atgccggcgc taattccact tgttcctcct ccaataatta atacatcagt tttgataaca
      301 ttatctttga tgcaagaaga tgaactaaaa agaaatgccg cactcataaa aaagtaaaat
      361 atagaatttt tatttgtttt cataagaaaa agattttaaa gatgttaaaa tatctatttt
      421 tttattgtct ctaaaggatt gctttctaac atgccatttt ggacgtcgtt ccatagattt
      481 ttttctataa tgtgagaaat atcgaaaatc atgactccat cagattcttg taaattcatg
      541 ataatagaac gagttaaatc tttacgttga ttataaaatt gacttgccaa aattcctcca
      601 taaaaagaat ttccgtctaa aatttttttt aattttttgc aagaaccttc tacacaatac
      661 catgtccctt gttgaaattt gaaatctgtt tcatttttta tcatcgggtg attttttaaa
      721 taatcttcaa ttgaaatggt agaataatag tttccaacgg tgaacaaatc caataattct
      781 ctaaaaccga aatttttata ctcagaagtt gcccatttgt attctttact agggtcataa
      841 tttttacttg caaagttgac ccctacttca taataggaag gataccatgc gcctgtataa
      901 actccaaaag aaatattttt atttactgat tttacttttt ctttagctaa agacataaaa
      961 tcataaatat ttttggctct ccattctatc cactttaaaa aatgtttacc ttcttttgga
     1021 taaaaattac cttttgagtc tttttgccat tcataaatat cattaggaaa attttctatt
     1081 ttttgtccta tgtattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncctt
     1201 ctacatttcg atataaataa gtttctaacc attttgcacc aattactttt gtatttgttg
     1261 gttgtacagg tttaccttcg ttccctactt gatcaatggc atttactctt ttatattctc
     1321 ctttcccatc taaagtgtat ccattgcagt taatatctgt ttggaatttt ttccctttcc
     1381 cttctactat aaatctcaaa gttctatcat gaatgatttg aacagacaca aatacagtat
     1441 cgcctggata gaaataatgt ttttcttgga caggtgcgtt accgtatcca gatttttgcc
     1501 cacttgaagc atgtgatgcc catctgtaaa aaggccgaaa ggctcttctg tctgcactaa
     1561 tacctccagt ttccatcatt attacttccc aggttaatcc aatatctgtt tcttgcccat
     1621 cggctcttcc tcctaaatat atagaaggat tatctaaata ttgtgctggt ttattgggat
     1681 tgattcgttg aggatcgaaa gaaatggttg gtaaaattac tgttccgcat attcctaacc
     1741 aatggtcgtt actagataat atttttctgt aatatgcacc aagaaagcaa ttgggtgtag
     1801 cacctggcgg gatttcattt gataaaaaat tttgaaaatc tatattttct tctattgaat
     1861 caatttcagt actatcatta ggttcttgaa tcgtatcatt ttgaggtgga aacatatcag
     1921 gggtaatggt tattccattt tcgttattgt tacaactaaa taacaaaata gctatactta
     1981 aaacataaaa aaatttacat gatgttttct ttgccataaa tctgtagatg ttataaataa
     2041 ttcatttttt ttaaataatc agtccaaata atataagctt ctggttgaag atggatgcca
     2101 tctttggtta aatttgcttt taattgtcct ttttcgtcag aaagcaaaga agcaatgtcg
     2161 atatagggaa tatttttttc ttcacataat tttttaagtt gactattaag ctggataatg
     2221 gtagaatttt taccttttaa ttcattataa gcaagggaag tatcatttac aggtagaaca
     2281 ctttgaatat acaattgtgt gtgaggagaa acagtttgtg ttatttcaat tattttttta
     2341 aaatttaaaa ttatttctgt tatatcccgt cctgcagtaa tgtcgtttat tccagccatt
     2401 aaaaaaattt ttttaggttt tgtttgtaaa atttcaggca aacgatgtaa aattccataa
     2461 gtgttatcac ctccgatgcc tctgttgaca atatcttcat tttttaataa aatagaccac
     2521 catccttgct ctgtaatact atttcccaga aatactatac tattggctct tgttttttct
     2581 tgacgatggg cttttaaacg gttcatataa taaggggatt caaataattt attagtagca
     2641 ggtttatttt cattaacata aggttttagt atttcaatcc acattttata tccttcgcca
     2701 gttaaatgaa gtccatcatt tgtaaattct ggttttaact gttctttttc attgcaaaac
     2761 actgaaaaaa gatcaatgta agttagattt tcttcttgac aaagttcttt taatttttta
     2821 tttaagaaca gaatatgctt ttctttgttt tgaagtcgtg gatatttttt aaaatcattg
     2881 ttaatgggca aaatgctttg aatgtatagt tgagtggttg gacttttttc taatatttct
     2941 tcaattatca tttgataatt atataaaatg atagaatcag gattgttttt tgaaatatca
     3001 ttgataccta ttaaaaggaa aatttttgaa ggttgagcaa ctaacatgtc atttaaacgt
     3061 gccagcactc ccaacgtaat atcaccagat attcctctgt tttttatctt cgaattttga
     3121 aaaaactcag gccattctcc ataatcagta atactgtttc ctaaaaaaat aatttcgttg
     3181 ttttcacgtg gaagtatttt gtaaatagat cttttttgtt catagtaaat tgtttgataa
     3241 ttgttttttt ctaaaatgtc tgaaattaca tcggggcaat tggtagtaat ggcgcttact
     3301 ttattgttta cttgagtaaa taaagctatt ttaggatcgt ccactgtcca tgtccataca
     3361 tctaaaccag cttctttcgc tttttttact aaattttcgg tcaatatttt ataatgtaca
     3421 tctaaaccgt caaaattgta ttttttacaa attgcaatta attcattttc cttatttttt
     3481 tcgctactta aataatagca ttttaaatgt ggtagttgtt ttttagcttc gattagcatt
     3541 gggagatgaa atgaaataat aacagcatcc ttttctttcc ctgatctttt taggatggaa
     3601 tttaaatttg aaataatttt ttttgtattt ttttcatttt cgtctttaat ttcaataact
     3661 acttttttac cttcagggag gatgttaagt acttcttgta agaaaggaat ttgttcgccg
     3721 ttttttagtt gtaaatcacg taattctttt gctgtagaag tagaaatgtt taagtttttg
     3781 ttggctgttc gtccagtcat ttgatcatgc atcactatta aggaatcatc tttagatttc
     3841 catacatcta attcgatagc atcggcattt aattcgaaag cttttttaaa agctgctatg
     3901 ctattttcta attcataggc agaagcaccc cgatgagcaa caatgcttat ttgagaagta
     3961 aggggaaaag aaagaaaaat aaaggttagt agaaagctta aaaaaattgg ttttaatttc
     4021 attttttaac tattttatta ttttaatcat tgtttgttaa agctttatta attcctgctt
     4081 ttacattttc ccactgatca tacataatca agtaaatgat atcaaaaaac atcaatccat
     4141 tcgattctgt tagacaaatg taaacagctt tttcacaatc ttgaggggca ttgtaaaaat
     4201 ttaaaccata caaaccgcca catactaata catctccctt tgtcacttct tgagcctgtt
     4261 gaagtgcacc ttgcactgtc cattcacctg aaccgtaaag ggttttacta taagctcctg
     4321 tcatataaaa atctaacaaa tcggcaattc cataattttt ataatttggg gtcgcccatt
     4381 tatatttttg agaagggtcg tattttggac ttgcccaatt cactccttcg ttgtaataac
     4441 ttgaatacca agatcctgta taaatgccaa gctgaatatt cttattaatg gttttaatag
     4501 tgtttctggc tctttcaaca aaatcatgaa ttgtctttgc acgaaattca agccataatt
     4561 taaaatatag accttcaatt cgattataag ttccatctgt attaggagtt gaatcccatt
     4621 taaaaatgtc atcaggatag tttgttaatt ttttcccaat gtaagtttca aatttttctc
     4681 gtgtaagatt gctaaaatca ctttcaatac tattaaaacg acaacggtca agtactatgc
     4741 catccaacgt tggataatta gttgcaagtt cagcaataat gcttaaaatg taattttgaa
     4801 cattttcatt tacaggatta aaaaatttag tagtactatt actatcatac ataatgtttg
     4861 ttatgccttg tgctccaggt aaatataaga ttgtcaacca ttcaatttta tcaggttcgc
     4921 gataaactat gcctgttcct gaattgctgg tatttcctcc tacaaaagta ttgatattgg
     4981 catgtacttt taaccctaaa gcatgtcctt tttcgataaa tgcagttaag taatcccacg
     5041 tagctgttcg ttctacgtct ccaactttcg taagagggtc tacaatgtta ctttgataaa
     5101 ggacatctcc agaggttccg cgtacatcta caataatttc tgtaaatcct gtattttttg
     5161 ctttttcaag ataaaattga atattttctt gactgttagc aaaatacttg aaatttgcag
     5221 aggcatcaat ccagagaatt ttaggttttt tttctactgg taaaaattca tagttatcat
     5281 tccattccca tttaggtaaa gggtgttctg aatccttaca ggaaaaaaat tgaattatca
     5341 aaaacaaaat taaacaaaaa ccacaatgtt tcttcataaa attatggata ttacaaagat
     5401 aaatttgata atccgcaaag tatcctaaat aaattcaacc gacgctattt tggtgatgca
     5461 atcttcgata ggttgcttgt tgctgctgta agctacaact ccgatttcag atcaagaatt
     5521 tataataggt cttcttgcgg ataatcataa agataaatta acatatctcg tttttttaaa
     5581 acgagatatg ttaattacaa tcgatttatt cgccaaaaat gttatctaca tcaatacaat
     5641 caaattcata agcacctaca ccaaaattag taataagtaa atacataatc attttataac
     5701 catcatcaga aactttaatt gccacatcac cagttgcatt tgtattacca gttactacat
     5761 tcggaggaga actaaacact aataaacgtg gatctttagg tgccaatgaa agatttgttg
     5821 gaatagttac atcatacaaa tgacaattat taccacctcc ataaagagct acaatagttt
     5881 gagcaagata atgagctcca ttaaaaattg cataatccaa tgactgtgga acgaaattag
     5941 aatcgtaacc tgctcctata agatcataaa gtgctaacac acttccatca gcattaacag
     6001 ccccaaagct acttgggtaa ccgctaataa ataaaattga gggataagta gctgagcgag
     6061 ctatggcatc gctttggaat gtccaattgt tttcaaaagc aactgagata gaacgtgtgg
     6121 gacctccatc aggagcagat tgaatgcttc catttttaac ttcccaaata tgcactattt
     6181 ttgattccat ttgagtaact gtaattaagg catcaccatc aaggttacct ctaacagaca
     6241 tttttcttcc catagctatt ccaccggtag gccaatctat taataaaact ggatttgcat
     6301 catttacgtt gttaaattta tatactttga agtttggtcc tgcattgggt actaaattgc
     6361 aaataagaat atgtcctact gaatcacttg tggcaaagaa atttgttaaa gagcccttaa
     6421 taggacctaa ttgcattctt ccttttggtt ctccggaaaa acgatccaca taaacattat
     6481 cttgattgcg ggtattaatt actaaataat taccactgac ggcgatagca gttgaataat
     6541 ggtcagcagg aggtaacccc atttctccta aacttttaaa ccatagttgc cgcgcacttg
     6601 ctggacgaat gccataagct accttttgtg gcgtgaaggg actcactgta taagttcgtt
     6661 gagttccatc aaaggctgtg acagtgtaaa ttactggttc attgaaattt tgtgctatag
     6721 aagtatcggg agaaatggta gcgtgatatg aaatagttat ttttggtttt tgatttgata
     6781 aatccattcc tccacttaca ataccgataa aattttgttc ttctaaaata aaacctttta
     6841 atcctacagc gggtagttca aattctttta tcatcgcttc tgaacttttc ttacgaactc
     6901 ctgtgatgta aatagtaatt ttcctaccac ttggagaagt aactgtatac ggtgttactt
     6961 ttgttaaatc aataatatca aaaggcggtg atacgtaaac attatttggc aagctcgctt
     7021 ttattttcat tttatcaatg cttgtctcat tagtgctttc tggtggataa aaccaaggaa
     7081 caataatttt tactgtatca ccatcatgaa atggatactt atttattgta gtatcaatag
     7141 taaactctcc ggttccatcc ataaaggtac ataccaattg cataatgccc gaattacttt
     7201 ctgtatctat tctttgaatt tcttcgggct cctcacatga gtgaaaaaga cacatcaagg
     7261 tgaggcttgt aataaataaa aatatgtatt ttttcataat tcaagttttt taccatcctt
     7321 cgatttgact aattgctaaa ttatttgcta tttcatttga tgggatagga agtcgataca
     7381 atttttcagg gaattgtcta tcacggtcat cgcattccac atatttataa gtaaattgat
     7441 tacttgcttt ttctatccgt aaaccatgaa aacgtactcc cgttaaaact tgatgagcaa
     7501 ttctccagcg acgtaaatcc caataatatt taccttcaaa agctaattct atttttcgtt
     7561 catgcctaat agcttcaatt agatcattac ctgtttttgt agtatggggt agtccaactc
     7621 gtgcccttat agtagcgaga tcttgattcg ctgaattatt ttctcctaac attgcacatg
     7681 cttctgcatg atttaaatat acttcagcta aacgaatttc aatccatggt tgcgtgcttc
     7741 taatatttac taaatcagtg ttgttttcat cgagccattt tttgaggtaa tatcctgtgg
     7801 ttgtttttcc tttaggatat tgttcttctc cgtattgtat aaaaccatcc ttacctccaa
     7861 catatggctc aatttttctt cctttccaat ttgcctcatt atacaagatg gaagcatgaa
     7921 aacgaggttc aagttgttca taaggaggag tttctgtcgt tccacttgta gaatgccatg
     7981 tgctccaatc tggttttcca cctgtagcta attcgtattc ttcaaccatt tcttgagtag
     8041 gagtggcttt tccacccatt tcagcttcat cgccacctgg tgcatattgt cggtcaaagt
     8101 tatgagaagg agcaggtaag gcataattaa attctaaaat agattcttta tttccagttt
     8161 tcatataaga tttaaaagcc tcttcataag taggtgctaa ttcataaata tttaaagcaa
     8221 aaactgaatc cgctgctgcc tttgcttttt cccaacgtct ggcaaacaac atggcacgag
     8281 atttaaaagc gaaagcagct cctttggtaa gtcttccttt gttagcatca tcccattgaa
     8341 ttggtagata ccgactagca aaatccaaat cattttcgat aaaattccaa cattcctctt
     8401 cagaactcaa tggattattt ttattagtcg ttggagcatc gagcaaaatg gcagttccgt
     8461 gtcgaattac taattgatga taaagatatg ccctaaagaa acgagcttga gcttcgtatc
     8521 gattttttat ttcattagag aaattagcat attctttaag acctaccaga aattcattga
     8581 cacgccgtat tctgttgtaa gcatctcccc atacacctaa taaattttga tcgggagtta
     8641 tttttgaagg ttcaacagca taatcatttg gagtgccatc gcctgcaaca tttgaaccat
     8701 attttagcat atcagttaaa ccttctgtta gtaatcctcc aaattgtcca ttcccaaaat
     8761 taccataagt actaatatag gtgtaaaagc catttaaata taaatcggca tttttttcac
     8821 tttgccatac cattttgtct gaatatctat cggttggcac tggatcaagg taatcccaat
     8881 cattacaact agttaaaagt gccatgaagg atagaattgt taatttaaat atcttctttt
     8941 tcatatagtg aatattttag atgtcaaaaa aattagaaag ttaaattaat cccaaaggaa
     9001 taagtttttt gttgtggata ataaccatta caaacatcag gtgcttcagg gtctaaacca
     9061 tttaaacgtt tatttgccca ggtatagaga tttgatcctg atacagaaac tcttgcagat
     9121 ttcattccta ttgtgtttac aaatttttgt ggaagaaaat acccgatttg taaatttttt
     9181 attcttaaat aagaaccatc aacaatccag aaagtagaag cccagttatt attccatcgg
     9241 gtttgagtag ataatcgggg aaattttgca tcaggatttt catatgtcca ggcattttct
     9301 actaaactta atggactatt tccatcatga taaaaaggac gtgtaaattc tgtattgtcc
     9361 catcctattc cctcataata acccattaaa gcaatgtcgc aaaaggctgc tcctgtgaaa
     9421 agtattgaaa aatcaaatcc tttccaggaa gcatgtaaat ctaatccacc cattaattct
     9481 ggaatattgc tttttgcgat ccacgctcta tcttgatcat aagtaatttt accatcccca
     9541 tttaaatctt tatagcggat atccccgcct tttgctcctt ctcgtacagt tggatagatg
     9601 gctgcttctt catcagattt aaaaagtcct aaggacacaa agccatcttt catgcccact
     9661 tgcaaaccat ttctttttaa gtagtcgggt atattagggc tatcatcaat atttaaaatc
     9721 cgatttcgtg cccagtttaa acttaaacgt gctccataat taaattggtt gattttattt
     9781 tcgtgagtta aaactaaatc aaatcctctt gcatcaactt ttcctgcatt gatgacactg
     9841 gggaaatttc caccaactga tggaggccaa attcctgctt gacttctcag aatatcagta
     9901 gtcaatttgt aaaaccaatc aaattccatt cccagtaagc cattccacat taacagatcg
     9961 aatcctgcgt tataagtagt agtaacttcc catgtattat tacttggtac tccagaagtc
    10021 attaatgctt tttgaggaat atttccaata actactgagg gatcctcaga aacccaagcc
    10081 atagttctaa gataagcaaa ttcaggagaa gctgcatcat tacctaattg tcctgctgaa
    10141 gcgcgtaatt taagattagt tacaaaaggt atcgcttctt taaaaaagtt ttcttcagaa
    10201 attctccatc caattgatgc tgcaggaaac aatgaccaac gggtttcagg tgaaaaacga
    10261 taagaaccat catatctacc cgaaacttct aacaaatatt tttgttcata agcataagag
    10321 gctctaaaca cgaaacctgc tcgaggcgaa gttttgctgg aaccactaaa ttgattttga
    10381 tctgcaattg atttagcatg atttagctct gcaaaatcat aaaaatcaaa atcttttaca
    10441 tttacattaa attgcttata tatgtaatta gattgttccc acaatgctaa agcactaata
    10501 tcatgtttcc caaaagtttg agcataatta atttgtccct ggaaagtaat tcgtgtagcc
    10561 tcagcaaaac cttcggacat agtagccacg ttgccaattg ctactggata catggtttca
    10621 tattttaatg aaggataagt tggcgtaggg cgagaagcta aacttacttg aaaaggggtt
    10681 ttaaaaacct tgctatgagt gtaatctctg tcaaagctac ccattgctct tgctgataac
    10741 ccttttaacc atggaatttc ccaatttaat gcaaagttac tttgaaaaac agacatttca
    10801 ctgtcattaa aaccagataa gtctctcgca gcgaccggac ttacttgagc acttgcagtc
    10861 tttgtggcag taggcaaacc attataagta agaggaacat aaggatgagc tctgatagct
    10921 tgttgaataa tgctcatata ctcatttttt cctgcaccaa aatagggcct attacgattt
    10981 tctttacggg caccgatatt gaaatttgcg gtcaaacctt ttactatgtt tgcatcaata
    11041 ttagaacgaa cattataacg attaaaatca aagtttttta cgtttccttt ttgatcatag
    11101 tatcctatca tcatgtaata tttaacgtct tttgttcccc ctgttgctga aaggttatga
    11161 tgttgagttt gacccgtttt aaaaatttca tccacccaat tggtattacc ataaacacct
    11221 tcgggatcgc cattgatcat tttattaacc atttcttctg taaatactgg agcctgttca
    11281 tccatttcta atgctttgtt ccaataataa gcatattgag gtccattcat aaattttggg
    11341 aaagaagtgt tttgatttat ttcaaaatta ccagaataag taatttgagg tttcatttcc
    11401 attccttttt tggtggtaac taaaataact ccattagccc cttgtattcc atatacagcc
    11461 gctgctgcag catcttttaa tatggaaata ctttcaattt cgtttggatc taactggtta
    11521 aatgaacgcg gtactccatc tataataatc ataggatcag tagcacccga agtagttgta
    11581 ataccacgaa taaccattga agcatcatct gacccaggaa tacctgatga ctggcgtgtt
    11641 accaatcctg ttactcgtcc ccctaacatt tgagaaagat tgacaggagg cgcttttaac
    11701 atttcggcac ctgaaatttg ggaaatagaa cctgttacac ttactttttt ttgtgtgccg
    11761 tatccaacca caactacttc ttgaagaagt ttgggttctt ctatcatggt aattgtcaat
    11821 tgtcggcttg ttcctacggg aacttcttgc ttttgatagc caacaaatga aaagactaaa
    11881 acggcttttt gatcaggaac tgataaggaa aaatttccat ttatatctgt tattgtccca
    11941 atactagttc cttttatcat taccgttaca cccgttagag gttcattgtt ttgatcaatg
    12001 acctttcctt gaatgtttat ttgtgcactc attgataaag cacaacttaa cattcctaag
    12061 agcacgaaaa cttttaagcc tcgaaactct aattttgata gattcttgtt tttcatagaa
    12121 taaattaatg tttaatgtag aaagttaatt aatcacttgt tttttaaagc tattttttta
    12181 aaattatttt atctatttaa gccctccatc ttattttttt cttcgtggct aaagcaacga
    12241 gaaacataac acaaaatact aaaataaaag cccttaaaac acctaaccaa tgatatccat
    12301 caggataaca tccttgataa ggtatgatta aaaatgtaag gttcattaaa ggaattacga
    12361 accaattggt agctatgtat gctaaaagtg gattacttcc tgctcctgca aaaagtttga
    12421 caaaatgtga tttctcattt ctttgacaaa cgtaatctaa gaaaattaac cataatatac
    12481 tgattccacc tgttataaaa agataagatg ctgtaacagg gtcttttttt attcctcctt
    12541 cagcaggttc taaaattaat ccaactaaaa tcatataggc tgctatttta aaaaaggaaa
    12601 gagaagaagg ggaatatttt tttactatgt aataaaaaca tgccaacaca ataggtgttg
    12661 aaatataaag aattactaac catcgattgt ataaagctac tataaaccat attcctaata
    12721 aaaaaatcaa gtgagaaata aatttttctc ctttcttttt tgaatgagaa gtattttttg
    12781 aattttctgc tgttagtcgc tcataaatta tatcgcccac tatagttgcc ggaatgatta
    12841 ataacatata atgagtcatt ctgaaaagga ataaccacga caaacctgaa atttgattaa
    12901 gggcttcatc aaaatgagta gttttgcaag taatttgtat agcaccccag atcattaaca
    12961 gtagcaaacg atttaattta tcacttcttg taaaatacca actaaaagct ccaaacaaat
    13021 aaacgtttgc taaaagaaga ataataatgt tacttctatg taaagaaaat tttaatccat
    13081 acacataatg cagaagtaat aataatgaaa cgatagcagt ccatcctatt attctccatt
    13141 tctttttttg ctgttgattt tctataatta cataaatact aaataaagaa agaaacccta
    13201 ttatagtgat aaggaataaa tcatatcctt taatataaat ccctaaaaag tgaaaattaa
    13261 caaaactctt tggaatagca gatgtttgaa aatgagcaat cagaatagca aatattacca
    13321 acataaaata acgctttata atactttgag caatattcca gaaatttttg ccagcttcta
    13381 tttttttatt taatgctaat ggaatggcaa cccccataca aaaaataaaa ataggaagaa
    13441 ctaagtcaac ccaagtaatg cctggtacat cagggttgaa aacatgagaa ggtggaggtg
    13501 tttgtctatg atacatccat gcaggtaata ctccaaaagg gatattacca gaaagaacca
    13561 tcccaaaaat agaaattcct cgtaagacat ctaaagcgac tgctcttttc atgagacaag
    13621 ttagtattag attaaataag gcaaggcagt aattttttga tgatttgttt aaatttttta
    13681 gtaataattt tctctttttg aaatatattt ttatcaaaag cactttttat aaggatttcc
    13741 ttataaaaag tgctttacaa agttgattta ttcaactatt aaacgtttga ataccttttc
    13801 tcctctttga gattctattt ctaacagata aatacctttt aattctttta cattgagatg
    13861 atcaacatta ttggcttctt taagcaattg tcctgaaatg ctaaataatt ttactttttt
    13921 tgcggtttgt gaaaaatgaa ctttctcttt agctggattg ggataaatat tcacttctat
    13981 cttttcaggt ggcatgggta atcctgttgt aatatctaaa ccttctactt tgtatgctcc
    14041 aaatccattg tttgtggaaa cgacataaat aataggattc ccttgttgat cataacgaac
    14101 ttctacatct cctgtgcctc ctacatttgc attagctcct aagcttggag tacgagcaat
    14161 aatttggcag gtactaaggt cacccggagt caaagaaata atttcagcac attctttttg
    14221 tcctccgtag gtaaataata ctatataatc taaattatat gtagaactat atcctatgta
    14281 ttttactgaa tttgaataca tagagatagt ttgttctggc tctgacaaaa cggcctcatt
    14341 agttattaaa tgaatttttt tcccgcctgc tttccagaat aaatctccat tgggtttaac
    14401 gccgatacct ggacatactg cgttcccatc gagtgaaata cttttgacaa gagttctttc
    14461 agttttccat tgaccatttt ccatctctaa aacataaatg tcagaggggt ttgctgcagt
    14521 agcagtataa atttttgccg ttccttctga atagtcacct actactgtaa ataaatctcc
    14581 tgttcgacca cctggcgctg catccggata atgcaatact actactggct cagcagttaa
    14641 gttatcgtac atataaattt taaagtttgt gttagcccct aagctggcag taataatttt
    14701 cccatcatca gttgctccta catcatttag tgggaaaagt ccatcaacaa tatttgcagt
    14761 attcagagca cagatagaat cacctgtcgt agcattgtat acaaaaatat ttttaccgct
    14821 cgttccttct ctggtagcca caaaaacgtg ttcattagag gctcctatac cacgtgtttt
    14881 atttgtagta gttaagaacc atgcaggtaa agaacctgaa gctgcagatt tttcccacag
    14941 agtagtaatg gtgggagtgg gctcagtttg ggcttgtaat gctgctacag taagacaagc
    15001 actcattaaa aaagtaattt ttttcatgat ttttgttttt tagattgtga atgaataaaa
    15061 ttaattaaaa aaaatgttta tttgcatgta gaagaaaatt attaagaaaa tgtatttaca
    15121 aagaaataaa aaattaatta taaagtcaat ggttttttaa aaaaattttt ataaataatt
    15181 tgtgtacatg aaaatttttt tattaccttt ttattaagaa aaattttaac atataatccc
    15241 tttatttttt ttataaatct gatatctttt ctaaggtttt ccagcattga taaagtcctc
    15301 tgggtacgtg gaaacaacct ttccattttc ctcctttcag attcaaaagc acttctccac
    15361 gacggttaag ataaccgaac cattccggat agagaggatc tttaaagtgt gaccatgtgt
    15421 aagcatggat ttttttaaac cattgtaagc attctttgtt tccagtcaat tgatatcctt
    15481 taatcatggc aatcaacgct tcaatgtgaa cccaccataa cttttgatca tattctaatt
    15541 gttggggtgg atatccttta cgatccatgt agtaaaaaat accgtcatgt tttttatccc
    15601 agccataatt aaccatttta agtgctattt ctacagcttt ttcgatgatt tggggacgat
    15661 ttaaccgttc accgacatcc ataagaaacc acatggcttc taaagcatga ccggggttta
    15721 ccaatctgcc atcaaatgag tctgataatt cattagttaa cgttacattt tcgactacaa
    15781 ttcctaattc tggtcgataa aatacttcta aaatttcatg agtaatattt tcaagagttt
    15841 gtaagaggaa tttttcatcc aataagtgct ctatttccag ggcaagatta gaaagaatca
    15901 ttggaagagc aaagtttttt aaatctcgag ttccgggatg aactttattc catttccctt
    15961 ttggattctc tttttttgat aatattttat cgaaagtagc ttttgcaatt tgctcgtatt
    16021 cttggttgcc agtagccaaa cttaattgac cgaaagccat agcagcaaaa gtataagaga
    16081 atatattata aggttcaatt aaaggttttc cttcccgtgt taatgaaaaa taccaattaa
    16141 attcgccatc atgaccatat ttttttaaaa actcagctcc ttggatagca cagtcgagcc
    16201 attcttgttt cttttcaact ttattatata aagtagaaaa catccaaact tctctccctt
    16261 gcaaccaaat aaatttatca gtatcaaaaa tattgccttg tccatcaaaa caagtgaaat
    16321 aaccaccaaa ttctttgtct tgcgacttat taagccaaaa tggaataaca ttatttagta
    16381 attcattctg gtattgcttc gctaatttac taaaattcat cattttattt gattttaaat
    16441 tatttattat aataaaattg aaattttaaa tttctttttt gagaataatc ttttcttgcc
    16501 ttttattttt ataatttact tttcttgaga atacagaaaa atttcaaatg taagttttat
    16561 tttaatgaaa taatgttcta aaaaataatt attgaatttc gcttcaaata taaaatatat
    16621 ttaataataa tccaaataaa aataaaaata ttttataaat attttttgaa aaatatttta
    16681 taataaaaat aaagaaagat ttttgaattt tattattaaa aaatttattt gttaatagaa
    16741 gatttcaaaa aaagaattaa atttgtaaat gcataaaaat tcttttttta aaaagaaaga
    16801 agcagttcaa tttgagaata aataagtttt ttaattgtga ctttttaaga aaaactaaat
    16861 gtgaaaaatt aataataaag aatatgaggt acaaacttat cgacgaaata gagttaggtt
    16921 ctaaaaatgc tctattaaaa ataaatatct tacaatatta tattaataat ggtgaaaata
    16981 gtttggctga tcttgccaaa gaaatgaatt taagtattcc aaccgtgagt aaacttgtta
    17041 acgaattaat tgaagaagga tatgtgattg actttgggaa acaggaaacc aatggtggaa
    17101 gacgtcccag tatttatgga ttgaatcctg attcgggtta ttttgttggc gttgatttaa
    17161 aaagatttcg aatcaattta gctcttatca attttaaagg ggaaatggtt gattcaaaac
    17221 taagtattcc ttttaattat gaaaatacgc aggaaagttt tgataccgtt tgtaaaatta
    17281 ttgatgactt tgtaaaccaa ctctccattc caaaatcgaa aattttaagt gtaggcgtta
    17341 atatttctgg acgggtaaat acgcagacgg gcatgagtta tagttattac ttttttaagg
    17401 aagagccatt gacagaaatt tttcagcagc gattaggttt acacgttact attgataatg
    17461 attctcgtgc tatggcttat ggagaattta tgaaagggga cgtaaaaagt gaaaaaaatg
    17521 tgttgtttgt caatgtaagt tggggtttgg gactcggtat tattgtggac ggcaaacttt
    17581 attatggaaa atcgggcttc tctggtgaat ttggacacat tagtgctttt gataatgaag
    17641 tgctttgtca ctgtggcaag aaaggctgtc ttgaaactca agcttcagga tcgtatattt
    17701 atagaaaatt tatagaaaaa gtgaaggaag gaaattcttc tatccttgca ggaagaatta
    17761 aaaaaggaga gagcatcacc caggaagata ttttagaagc tgctttaaat gaagatatgc
    17821 tggcaattga attagtggaa gaagtaggat atacattagg gaaacacatt gctggtttaa
    17881 ttaatctttt taatcctgaa ttagttatta ttggaggagt ggttgctatg actggtgatt
    17941 atttgttatt gcccatcaaa agcgccgtaa aaaaatattc tttaaatctg gtcaataaag
    18001 ataccaccat caaattaagc aagttaggtg atcttgccgg cgtgatagga gcttgtatgc
    18061 tggcacgaag caaaatgtta gaattaattt aatcgatgta ttacgaaaac ttataggtat
    18121 ttataaattt tctatagaat cgcttggttg aaccatttca taattttctt cattgcttgg
    18181 ttgcaaaaaa acatttatcc tattttttaa tattatgtta ctgataatca aaagaataat
    18241 tataaaaata aatagtccaa ttaataattt attgcttgtt ttcattgttg attatttctt
    18301 agtttattga atagattgtt gctgattata ttcttcataa agttttttaa tttcttcaag
    18361 gctgatatca agcaattgca aatttttaaa aaaaactggc aattcttttc caacaaattg
    18421 ttttcgcttg taatctataa ttttttcgat agcatttttt gtaacaaaat atcctatacc
    18481 tcgtttgttg taaataattt catttccttg caaaaagtca taagctcgca ttatggtgtt
    18541 aggattcacc tccaataaag cacttaattc tcgtactgag ggcaatcgtt cttcttcttt
    18601 ccaacgacgg agcaagattt gttcgcaaac ataatttgca atttgcagat aaatcggctg
    18661 tgaatcaata aattccatta ttttatttct ttttctgtta gacgaaaata gctaactatc
    18721 cagcagaaag gaataagtaa atagcctata attttccaaa cgaatatcat tccctttggg
    18781 aaaataattt catcccaagc catttgaaaa ccgtcatact gattcaatgc tgaaagtaaa
    18841 gaacccattg tcactttaaa aaccgtgtat tcaatcattc caaatagaat acttatcatc
    18901 ataagcgtta acagggtttt tgctattgaa tttcgtttga aatagaggga accaagtgta
    18961 aagatagctt gtgaagtagc aaaaactcca aaatttttca gcaaataagt tatgtcgaaa
    19021 tcagaattta gtttagaata ggggaagaaa aattgaaaag aaaaaggagt acttattcca
    19081 taaatcaaat ttcgcagaat agttccgata aaatttccta taactgaggt aataattatc
    19141 attaaaaaga aataaaaagt attcagcaga aatacagtga ttaatttttc aacctgagtg
    19201 gctggtatca ttaaataatg tgtaccattg gaagaatagg caaaaatttt gaaaaaacgc
    19261 gtggcatata ctatgccaac aataagcgta atcagttgtg cagttccttc acctttgaaa
    19321 aacatgaaaa ttaacgtcaa aatactccaa agataaattt catagttttg attttcaaca
    19381 aaatatattt ttagaagtaa atcaattctt ttgaggctga atgtttgata catatttttt
    19441 attttaatct tatttaaaat tgttcggttg atttgaaaaa atttctttta ttcgttgttg
    19501 gttttgcaaa acagcattaa ataaaagttc aatatctaac ctgctttctt cacctgtttt
    19561 gttttcaaca acctgataag taccacgaag attttcttcc gcgtaaagta cttcctcctt
    19621 ttcttctttt tgattgctga ctttgaacaa gagttttttg gtaatatttt cagtgaattc
    19681 attgaaaaca atttcgtgtc cattcattat aacaatgctg tcaatcaagc tgtctaaatc
    19741 ccttacttga tgcgtggaaa tgataataca acgattttct tcaatggcca gagctgccat
    19801 ttttctgaat tgagcttttg aagggatatc caatccattg gtgggttcat caagcaaaac
    19861 aagactcgta ttggctgcca aaccaaaagc gataaagaat tttttctttt gcccataaga
    19921 aagctggttc atctgtgttt ttgctgattc catttcaaat tcagttaaat attgatgaaa
    19981 ttgttccgag ctaaaatgag gataaaaagg agcatagact ttttcataat tctccatgct
    20041 caaattaaca ggtggcaatt cttctggaag gaaaaatatt tcctgtaaca tttctggatg
    20101 gcgtttccgt ggttcaacat tcattacgct gatttcacct ttcaatggaa aaaccagtcc
    20161 agatatcatt ttcaacaagg tacttttgcc ttcgccgttt ttccccaata aaccgtgaat
    20221 atgtccagcg aacaatgata aatttaagtt cttaaacagg tgagaagaat ggctgtaatt
    20281 gaattccaga tttttaatat caatcataag aatattttaa ttgattagtg tgttagtata
    20341 atagtacact acaaatgtaa tatcaaaatt tattattatg caaaaatatt tagttaaatc
    20401 ttgttaattt gcaaaatatt tttgtgattg gttgattatg agcatgtaaa atttaataga
    20461 taacgaaaaa atgtgagaat agtaggaata acatgtttca tttttccata ctataataat
    20521 aattatggag tggaaataaa tttattgaaa ggaaaatccg ctttaagatg aaaaaatcaa
    20581 atttttccct ggtataaaaa ttttacttaa aggcaaacca aaatattaaa cgcatttttg
    20641 aatgttttta ttccctcccc cacaacaatt ttattacact aaaaataatt ttttctctta
    20701 acatcatata atctgcgaaa gcttaggaga tttaattgaa cgaaacaata actcatcata
    20761 aggatggcgt ttatgcaatt cccaattttc taaagctatt tgtttggaag agtttgcata
    20821 gcaatattca cacaaatgtg gacaggtatt atactcgcca atgtctttgc tgacaatgca
    20881 tccacaaaat tttctttgtc ctttgtcttt attatctttg gtttttacaa aggttggagt
    20941 gggatgaaat atatctgctg gaatgatttt tacggcaaga aaatccatca actttttgtc
    21001 ctgatagaaa agtttaatga tcaaatcatc atcaatacat ttattatgct ttataccata
    21061 tttttccaac gaaatttgtt cggcacatgt tgctaattcg aaattccaat ttttattcaa
    21121 agcttggagc cctgctgcaa attcattcat ggttcgttcg ttaaactctt gataatgaat
    21181 agaactgttt cgcagattgt tttgaacttt tttgtagatt ttaatatcag caaaactgaa
    21241 aaccagtttt tgagtatgat tttttaattg atttccaata ttttctattt ttctcaataa
    21301 ttcatcaatt ccaatcttat cagttaaaat caggggatcg aagcgccaaa tcactttttc
    21361 tttgccaatt cgttcggata attgctgaaa agtttctatt ctttgttgga cattggggac
    21421 acgaggttct aatttttctt tgtcataatc attcaatgtg aattgaaaat aataattaaa
    21481 tcctttttcc tcaaagaaat ctaaatattt caatagcggt tttggatttt ttgaccagaa
    21541 cacaaaaaat cgtgcctttg agaaagcaac ataagaagaa acaccattaa aaggattgtt
    21601 ccatttaaga taaccttcat tgatcctttg gattaaccaa tcggaataaa aagcaggaat
    21661 atccgtagca cggctggctg aaatgataat gggggctatc gcttctatca tttcaccatg
    21721 ttggtttgtt attttaactg tatcccactt cattaatttt caattgatta aaaaaacaag
    21781 aataaagata ctaaaaaact tactacattt gacaaatcat gagtaaaata ttttcaaatt
    21841 ggttttaact gagaaggaga atgaatttta acggagtata taaatctgat tatcagttga
    21901 ttttgataaa atcatgaaaa aaacctcatt tttaacaaaa aattttttag aaattaagaa
    21961 taaattatta tatttgcata aaagaaagct agccgcattg ttctgagact ggaaaactca
    22021 tcagtgaaat attcattccg agaaaggtct ggctttcaat ggcgggctgc gccttcgggt
    22081 atccgtcagc aatggcggac ggcagattac agagaaagct aacgtctcaa atcctattta
    22141 taggagtttt tctcatgaag aacaatgcgg tttttttatg ccagcctttc ctttatcctt
    22201 agttccaatt agaaatatat ttagcatata ttgactttat tagatgaaag ataaaatgct
    22261 atttttggat gggatgtttc aatttcaacc ttatttgttt tttactctta agagggcgtc
    22321 ccaaaagggc actttctttt taatattaag cagctcgatc aaaggttaaa ttttaatttg
    22381 gagtagcttt gtttttaatt ttttggaaca agcgaagaat aagcataaaa acaaacgtat
    22441 ttatcagtat tttttgccga tttgttgatt ttcttgcttt tttcagcttg tttgttcatt
    22501 ttacggcata atttcctata ttgaaaactg tagcaaagag agcaaaatca gctttaattt
    22561 tttcctgtcc aaaatatcga aatcggaaat aagttttatc gtatttcatt tatccgaata
    22621 ccgcttcata ccaagtttcg atctaaaatg aaatattttt tagataaagg acaaaagtta
    22681 tagattttat ataatcaggt ttttatattt aattcattta ttcctttgca aaccactttc
    22741 cttgtttaga gggtaaatat taaatagtca atccttaaaa agtctatttt taaggattga
    22801 ctaaataaga aagattattt ttacaataaa caaaatcaat tgaaattctt ttcaaatgta
    22861 atctcttttt attctttatc tcgtaaatca cgactggaat catttaaaaa ctgttctcac
    22921 cgttctggct gttctttatg tctgtgtatt tctttcgtac tgattgatct gttttttgga
    22981 gtacgacggg caaagcgatt gcatatcgtt cgtacaagtt ccaattatcg tcataccatt
    23041 actaaactca ccaactattt tggtttaatc gtaagaattc tccgatgctt gcgtcggggt
    23101 agcttttgtt tttatttttt tagaaattcc ggtggtgaag ccataaaatt ggcgtaatac
    23161 ctcgctgctt gcggtgagga tagtcaattt taaggaattt ctttattaat attttcaaaa
    23221 actatttttc aggaatattt tgtagcgttc caagcagata a
//
LOCUS       DAOSAS010000007        23097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_1603_length_23097_cov_13.303272, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000007 DAOSAS010000000
VERSION     DAOSAS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 23097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23097
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1603_length_23097_cov_13.303272"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..2054
                     /gene="thrS"
                     /locus_tag="PLR72_00725"
     CDS             111..2054
                     /gene="thrS"
                     /locus_tag="PLR72_00725"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOK35768.1"
                     /translation="MIKITFPEGLVKEFPKGITGQEIAENISPKLAQEALAIGVNDEI
                     WDLTRPIENDAKVKIFTWEDEEGKHAFWHSSAHLMAEALEELYPGIKFGIGPAIENGF
                     YYDVDAGDTIIKETDLPQIEEKMKELAARKEPIIRKNISKADALKIFEEKGDEYKIEL
                     IQDLTDGTITLYSQGNFTDLCRGPHLPDTGKIKAIKLLSVAGAYWRGDEKRKQLTRIY
                     GITFPTKKMLDDYLVLLEEAKKRDHRKIGKELELFMFSDMVGKGLPIWLPRGTDLRLR
                     LEEILKKIQRRFDYQQVITPHIGNKQLYITSGHYAKYGKDSFQPIHTPEEGEEYLLKP
                     MNCPHHCEIYKFKPRSYKDLPLRIAEFGTVYRYEQSGELHGLTRVRSFTQDDAHIFCR
                     PEQVKDEFLKVMDIIFIIFKALDFNNFEAQISLRDPNNKEKYIGTDENWDKAEKAIVE
                     ACEEKGLKARVVLGEAAFYGPKLDFMVKDAIGRRWQLGTIQVDYNLPERFELEYTGED
                     NQKHRPVMIHRAPFGSMERFVALLIEHTAGKFPLWLAPDQVVILPISEKYNQYAFEVE
                     KILKEKSIRAIVDDRNEKIGRKIRDNELKRIPYLLIVGEKELVGREVSVRRQGEGDKG
                     TMKIEDFAQLILNEINTLLKQFD"
     gene            complement(2212..3702)
                     /gene="rseP"
                     /locus_tag="PLR72_00730"
     CDS             complement(2212..3702)
                     /gene="rseP"
                     /locus_tag="PLR72_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOK35769.1"
                     /translation="METFLIRALQLIFSLSILVIIHEFGHFIFARIFKVRVEKFYIFF
                     NPSFSIMRAKKINGKWKFKFFAKNVPPNERPKRDADGDIMLDENGKPIMEPIPLSELP
                     DDDWRKYPESTEWGIGWLPLGGYCKIAGMIDESMDKTQMKAEPQPWEYRSKSVWQRLP
                     IITAGVVMNFILAIVIYSGILYKWGQEYLPIQNAKYGLQFSETFLEHGFKNGDKILSI
                     DGENCEKLSDVIEKILIDGAQNVTVLRGDTQIVNVQLPKDFPQKVLASGEESLISYRF
                     PFVVDSVFANTPAWQAHLQRGDSVVAINGKPLYIFQDIASQLSQYRDSVITLAYYRNG
                     SLHESKIQLDETGKLGVAVRPYIDFFETTRIEYGLLASIPAGVSLGVETLASYVKQFK
                     LVFTKEGSKQLGGFGAIGKLFPKKWDWQIFWSMTALLSVILAFMNFLPIPALDGGYVL
                     FLLYEIFTGRKPSEKFLEQAQTIGMLLLLALLIYANGNDIYKAIFK"
     gene            complement(3823..4986)
                     /locus_tag="PLR72_00735"
     CDS             complement(3823..4986)
                     /locus_tag="PLR72_00735"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOK35770.1"
                     /translation="MSETKKQITILGSTGSIGTQALQVIEQYSDKFEVYALTANNNVD
                     LLIEQARKFHPEMVAIANPIHYEQLKKSLAELPIKVFAGEEAISQIAEIQSVDIILIA
                     IVGYSALKPTMHAIQAGKRIALANKETLVVAGELIGQLVEKYHAMILPVDSEHSAIFQ
                     CLTGEGNNDIEKIILTASGGPFRTKSFKDLQKVTSAEALKHPNWTMGTKITIDSATLM
                     NKGFEMIEAKWLFGLEPEQIEILIHPQSIIHSMVQFTDGSVKAQLSIPDMKLPILYAF
                     TYPERFNTNFSRINFNIISQLTFEKPDTERFRNLLLAYEAVHQGGNMPCILNAANEVV
                     VDAFLKNKIGFLQMSEIIEKIMSKALFIQQPTYEDYVNTDKEVRILTYELINK"
     gene            complement(5022..5891)
                     /locus_tag="PLR72_00740"
     CDS             complement(5022..5891)
                     /locus_tag="PLR72_00740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOK35771.1"
                     /translation="MKKKKGKISLLLQKLRFKYRVSVVNENTLEESWHIHLSRFTVIL
                     YFFLFTVLTFVLLTILIYVTPLRYYLPGYGETGDRAKIIQESLETDSLMKVVSQQSAY
                     IELIKKIINEEIQPDSISSLDSVTLSKKSQELLEKSKKEEEFCKKFEEEEKFNLANIS
                     INKNENTYVFFRPVQGVITSSYSLQNHRYGINILTPPNQSVMSVLNGTVIFAGFTFDF
                     DWVIQVQHENNYVSIYKNNTRLLKKVGNVVKAGETIAITGENNHSNKAKKEFYFELWQ
                     QGKPINPEEIIIF"
     assembly_gap    5968..6067
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6068..6471)
                     /locus_tag="PLR72_00745"
     CDS             complement(<6068..6471)
                     /locus_tag="PLR72_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOK35772.1"
                     /translation="MMGPIILAYIGIGLMVGLSGVGSAVGVTIAGNATLGALKKNPDA
                     FGNYMVLSAIPGTQGLYGFLGYFLLSPHLVPDMTWGAAAAVFGGSLALALTGLLSAIR
                     QGQVCANGIASIGSGYDVFGRTLILAAFPELYA"
     gene            complement(6519..8354)
                     /locus_tag="PLR72_00750"
     CDS             complement(6519..8354)
                     /locus_tag="PLR72_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOK35773.1"
                     /translation="MIVKMTKYTFLVYHKQYIEFLEKIREIGVLHVIEKPEGIPENDA
                     LREKMLLSARIKIALKQLEKRLSKEIIPLPADPVKDGLEVLINIENKLAQKESLEQKL
                     QQIEKERDRMEVWGIFSFERLHQLREAGYIINFFSCNIRKFDQEWEVIYNAFEIDTVG
                     STRYFITITRPGEVVDIDADPIKLSDRTAPELDLQISEIRKEIEEVKQQLIKMAVEDY
                     NTLKSFQVETFETIDFTKVVLNTEPTIEEKVMLIEGWCPEDSEEELQNFLNDSEIYYE
                     TSEPVGEEKVPVKLKNNRFARIFEFIGNLYDLPNYYEIDLTPFFAPFYMLFFGICLGD
                     AAYGLLLVLFAIFLRKKVNDAIKPVLSLMICLGISTVIMGIITGTFFGFNLIEAEIPW
                     LEKLKKIMIDSNQLFYISLILGVIQILFGMIIKAVGKVLRYGWPASFSTWGWLSILIG
                     CGGTFVLNSYAGLPFTIAKWLYIVFGGIGVIFVFLLNNIHKNPLINIGSGLWDTYNMA
                     TGLLGDTLSYIRLFALGISGAVMGFVFNDLAINISKGIPFFGPIVMIIIMLIGHSINI
                     FMSGLGAFVHPMRLTFVEFYKNAGFEGGGKKYKPFAHYKEEKSII"
     gene            complement(8351..8956)
                     /locus_tag="PLR72_00755"
     CDS             complement(8351..8956)
                     /locus_tag="PLR72_00755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOK35774.1"
                     /translation="MAIQFQYNKTSLQNLEKNLKIRVRALPTIKNKESALRTEVKKAK
                     DDIKKINAEFEARIAAYDKMLALWGEFDTSLLEVEDIKMSIKKIAAVRIPVLDEVVYK
                     TKSFSLFNAPKWYLDGLALLKELAQIAIEHEFYIQKLNLLEHERKKTTQKVNLFEKVQ
                     IPGYEDAIRKIKRFMEDEENLSKSSQKIIKSRHEKIEGRNI"
     gene            complement(9000..>9275)
                     /locus_tag="PLR72_00760"
     CDS             complement(9000..>9275)
                     /locus_tag="PLR72_00760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224009.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOK35775.1"
                     /translation="REDHPQVMNAAVRLYADAANAKTKLENGFDLTDYDERALAFAKE
                     YSEKILAIDVNIDTTTMLDVTWKLFAKYFKPSEVNIKQDLVDKYWRN"
     assembly_gap    9276..9375
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9376..10178)
                     /locus_tag="PLR72_00765"
     CDS             complement(<9376..10178)
                     /locus_tag="PLR72_00765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560284.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOK35776.1"
                     /translation="MATKAFQKIYTQISQITKATCSLHATNVGNDELATVNGKLAQVV
                     KIIGDEVTLQVFEGTEGIPTNAEVIFLGKAPTLKVSEQLAGRFFNAFGEVIDGGPEIE
                     GEEREIGGPTVNPVRREQPSELIATGIAGIDLNNTLVSGQKIPFFADPDQPYNEVMAN
                     VALRAQTDKIILGGIGLTNDDYLYFKNVFNEAGALDRIISFINTTEDPPVERLLIPDM
                     ALTAAEYFAVDKNEKVLVLLTDMTLYADALAIVSNRMDQIPSKDSMPGS"
     gene            complement(10232..12001)
                     /locus_tag="PLR72_00770"
     CDS             complement(10232..12001)
                     /locus_tag="PLR72_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOK35777.1"
                     /translation="MSTKGKVKGVVSNLVTVEIDGPVAQNEICYIELDGKMLMAEVLK
                     VIDTNAFVQVFESTRGLKVGNNVNFEEHMLEVTLAPGLLSKNFDGLQNDLNKFSGIFL
                     QRGEYNFPLDTEKLWNFKPLAKPGDEVEAGSWLGEVDENHQPHKIMVPFAFEGKYTVK
                     TIVPTGDYKILDTIAVLINEAGKEIEVNMTQKWPVKKAINIHTEKPRPFKLLETGVRV
                     IDTANPIVEGGTGFIPGAFGTGKTVLQHAISKQAEADIVIMAACGERANEVVELFVEF
                     PKLTDPHTDRLLMERTIIVANTSNMPVAAREASVYTAMTIAEYYRAMGLRVLLMADST
                     SRWAQALREMSNRMEELPGQDAFPMDLSAIIANFYSRAGYVQLKNGKTGSITFIGTVS
                     PAGGNLKEPVTESTKKVARCYFALEQARADSKRYPAINPIESYSKYFDYPEFEEYVTE
                     HISPDWTNMVNDMRTLLQRGREVQEQINILGDDGVPVDYHITFWKSELIDFVILQQDA
                     FDEIDALTPIERQHYMLSLIMNICNADFKFSNFVEVMDYFKRIINICKQMNYSVFHSE
                     DFEKYEKELNKILEERKIVNSSN"
     gene            complement(12080..12943)
                     /locus_tag="PLR72_00775"
     CDS             complement(12080..12943)
                     /locus_tag="PLR72_00775"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOK35778.1"
                     /translation="MNYYYLIAGLPDLHIEDTKLFLPLLDFKKELLVQLAPSDVELIK
                     LLFAKYDNQNFLLYLANKEAELNPLGNLTSEDWQQLITLMKETEHPNDKRLLPYIQQF
                     YAVYYEEEFWSEGISKEDYLSTLYYEYAMNNNNDFLKSWFEFNLNINNILVAVACRKY
                     GIDQKKMIVGNNEIAQILRTTNARDFGLTNIFENLETLLQIAEEKDLLQREKEIDAFK
                     WKWLEENTFFHYFSVEKILAFVIKMEILERWQPLSVEKGTQIFRTMVNHLKESVNFEE
                     EANVTSRRYFR"
     gene            complement(13022..13621)
                     /locus_tag="PLR72_00780"
     CDS             complement(13022..13621)
                     /locus_tag="PLR72_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35779.1"
                     /translation="MNTRLQELTDKIYQEGVEKGNAEAKAIIEKARAEADEIIKKAHE
                     EAAQIISEAETKAANIAKNTQAELRLFAQQAVNALKSEIANLICGEIVSNSVKAATAD
                     KDFMQKVILTLVKEWAKNQAITIETKDEKALTDYFMANAKDLLNKGVKITKVNNAKAD
                     FTIVPEGAGYKITFGEEEFIAYFKDFLRPKLVDMLFEKM"
     gene            13886..14368
                     /locus_tag="PLR72_00785"
     CDS             13886..14368
                     /locus_tag="PLR72_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOK35780.1"
                     /translation="MDILLIILSGIFVFLGFIGCILPILPSVPLSYIGILLLHFTSKI
                     QFSTQFLVIWGIIVLLSQLLDYFVPILGTKKFGGSKKGIWGSMLGMVIGLFFAPWGII
                     IGPFVGAITGELIDGKTNVQAIRAGLGSFIGWIINTACKLIIAGFFIYYYFEALIKVY
                     "
     gene            complement(14355..15737)
                     /gene="hisS"
                     /locus_tag="PLR72_00790"
     CDS             complement(14355..15737)
                     /gene="hisS"
                     /locus_tag="PLR72_00790"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOK35781.1"
                     /translation="MQKPTIPKGMRDFMPEEMARRNYIFDTIKNVFCRYGYQQIETPA
                     MENLSTLLGKYGEEGDKLLFKVLNSGNFLADISDNELLKHDTVKLSNKICEKGLRYDL
                     TVPFARFVVQNRDKITFPFKRYQIQPVWRADRPQKGRYREFYQCDADVVGSDSLVNEL
                     ELVQIVNEVFEQLKINVTIKINNRKILAGIAEIIGASQKILDITIAVDKMEKIGLQNV
                     NEELIEKGISPEAVAKLQPILTLKGNNAEKLDQLKLILVSSEIGLKGISEIETIFRLC
                     SNVDIKFFPELDLTLARGLNYYTGAIFEIKALDVKIGSIGGGGRYDDLTGVFGMKGVS
                     GVGISFGADRIYDVLNQLQLYPQTLQSQTQILFTALGEKELIYSFSWLQRLRSFGFNV
                     EIYPEPAKIKKQLDYANNKNIPFVAIVGENEVVSEKVMLKNMLTGNQQLVTFDELKKQ
                     LSDKLNLNIP"
     gene            complement(15801..17009)
                     /locus_tag="PLR72_00795"
     CDS             complement(15801..17009)
                     /locus_tag="PLR72_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOK35782.1"
                     /translation="MLYSFGIHVYGILIFIASLFYDKARELYRGQRNALKVLREKIDS
                     QSSYVWFHAASLGEFEQGRPIIEKLKKEHPETKILLTFYSPSGYNVRKNYSCVDVVSY
                     LPLDTRKAAQEFLKIVKPTKAIFIKYEFWPNFLLEMEAEKIPVYCISAIFRPNQIFFK
                     KYGKWYLQLLNTFSHIFVQDNLSAKLLNEHGIQKVTVCGDTRFDRVYALSLEPKKIPL
                     IESFARNSKVIVAGSTWPEDEELLIRYIKLHPQIKLILVPHEIDKSHLYDIFKLLEGK
                     YIRYTEATEINIHTFNCLVVDAIGFLSSIYQYGKVAYVGGGFGAGIHNILEAAVWNIP
                     VVFGPNYQKFREAHELIAEGGAFSISDYDSLEKKFDELLDDEEAGKRAGAYILKNVGA
                     TEKIYHLIFS"
     gene            complement(17180..17449)
                     /gene="rpsO"
                     /locus_tag="PLR72_00800"
     CDS             complement(17180..17449)
                     /gene="rpsO"
                     /locus_tag="PLR72_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOK35783.1"
                     /translation="MYLTAEKKKEIFEKYGKSNTDTGSPECQIALFSYRIDHLTQHLK
                     SNKKDYSTERALVKLVGKRRRLLDYLKEKDINRYRAIIQELGIRK"
     gene            17926..18084
                     /gene="rpmH"
                     /locus_tag="PLR72_00805"
     CDS             17926..18084
                     /gene="rpmH"
                     /locus_tag="PLR72_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOK35784.1"
                     /translation="MKRTFQPSQRKRRNKHGFRERMATANGRRVLAARRAKGRKKLTV
                     ADEGRHKR"
     gene            18242..19084
                     /locus_tag="PLR72_00810"
     CDS             18242..19084
                     /locus_tag="PLR72_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOK35785.1"
                     /translation="MNFKKFWKETFAGFVVKNVLLAIVVFILLSWVTLICIDFYTHHG
                     EAEIIPDLRGAYVEEAEILLSKKNLYPKVIDSVYIRDKKLGTIVEQIPPPNSTIKRNR
                     PVYLIINSRQVKQIPLPNVIDVSYRQAIAMLSSVGLSVEGVQYLPSEYKDLVLDVKYK
                     GSSLLPGTRIPESSSVILVVGSGYGDSLSVVPQLKGLTLQEAKEKAFLNSFVIGSTLY
                     DILPNGNDNDYFIYRQRPSAGELANVGSRIDVWLTTSKKRLKETFDENDNSLDSNGED
                     EIFF"
     gene            19181..20230
                     /locus_tag="PLR72_00815"
     CDS             19181..20230
                     /locus_tag="PLR72_00815"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOK35786.1"
                     /translation="MLEVTKWEELIESAEELEFYEHYRFKVDKRQSPLRIDKYLVNCI
                     ANISRNRIQEAAENGNILVNEKPVKSNYKVKPGDIITIVMIYPPSHFEIIPQNIPLNI
                     VYEDEELIVVNKQPGLVVHPGIGNYDGTLLNALAYHFKNQQDIDFNDPRIGLVHRIDK
                     DTSGLLLIAKTEDAKTQLGKQFYDKTTERRYQALVWGNIKEEEGTIEGSLARDPRNRM
                     LFSVFSEEENSSAKRAVTHYKVLERLAYVTLVECKLETGRTHQIRVHMKHIGHPVFND
                     ERYGGNEILRGNPSTSYRQFVKNCFEICPRQALHAKTLGFIHPKTGEFMNFDSELPAD
                     MEQLLEKWRNYAKTI"
     gene            complement(20287..20736)
                     /gene="rpiB"
                     /locus_tag="PLR72_00820"
     CDS             complement(20287..20736)
                     /gene="rpiB"
                     /locus_tag="PLR72_00820"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOK35787.1"
                     /translation="MKPLTELKIAICSDHAGYELKNELIEYLKIKNLAALKDFGTYSA
                     ESCDYPDYAHPLATAVENGDFDFGIALCASGNGVNITVNKHKGIRGALCWMSEIAFLA
                     RKHNDANVISLPAHFISQEEAKKMIDIFLTTDFEGGRHQRRIEKILC"
     gene            complement(20771..21922)
                     /locus_tag="PLR72_00825"
     CDS             complement(20771..21922)
                     /locus_tag="PLR72_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOK35788.1"
                     /translation="MAKILVIDDERSIRNTLKDILEFEKHKVSLAENGKQGLEIAQNE
                     AFDLIFSDIKMPEIDGIELLTLLKEREIEVPIVMISGHGTIDTAVECIKKGAFDFIEK
                     PIDLNRLLITVRNALERKELITESKTLKKNTEKKYQIIGQSPAIEKVRRLIDRVAPTD
                     ARVLITGDNGTGKELVARQLHQKSNRANAPFIEVNCAAIPSELIESELFGHEKGAFTS
                     ALKQRIGKFEQADEGTIFLDEIGDMSLSAQTKVLRILQENEFTRVGGDKIIKVNVRVI
                     AATNKNLINEIEKGNFREDLFHRLNVIPIHVPSLEERKEDIPLLITHFSELICYEQGR
                     QVKEFEPEAVSLLQQRNWPGNVRELRNIVERLIILGGSKITKEDVELFV"
     gene            complement(22017..23024)
                     /gene="porQ"
                     /locus_tag="PLR72_00830"
     CDS             complement(22017..23024)
                     /gene="porQ"
                     /locus_tag="PLR72_00830"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOK35789.1"
                     /translation="MKRKISLSLLFWLNINILIYSQAGQGVYKFLDLPVSSRIAGLGG
                     TNVSIRDNDLNFAFRNPALLTANTHHSIALNYANYLSDIQFGSVIFGENFKDKHYTAY
                     GIQYVDYGTFKETTEQNEIIGEFTAKDMAFYIMYAYPLNEKITVGGTFKPVYSVYERY
                     SSFGLALDAGISYNDINNLFSLGLVLRNVGTQLKGYYSDEEGQHYEALPFDIQLGATK
                     KLAHAPLRFSFTLHNLQNWDMEYLSSTGTKKIDFFDNLFRHFIVGLEFIPSNNFYLIA
                     AYNYRQSKEMKIEDFKSMAGFSFGGGVKLYKFQVGFGVNTFQVGNYSYQFSIATSLDA
                     F"
BASE COUNT         7642 a         4018 c         3397 g         7840 t
ORIGIN      
        1 attaaaaact cagcacacct aacattccaa cttactttcc ttatttcctt ttgaagaaaa
       61 aaaactatct ttgcaccatt aaattaatac ttaatggcaa aatttttaaa atgattaaaa
      121 taacttttcc tgaaggttta gtaaaagaat ttcctaaagg tattactggg caagaaattg
      181 cagaaaatat tagtccaaaa ttagctcaag aagcattggc tattggtgta aatgatgaaa
      241 tatgggattt aactcgacct attgaaaacg atgcaaaggt aaaaatattt acatgggaag
      301 acgaagaggg taaacatgct ttctggcatt cttctgctca tttaatggct gaagctcttg
      361 aagaactgta tccaggtatc aaatttggca ttggtccagc cattgaaaat ggtttttatt
      421 atgatgtgga tgcaggcgac acgattatta aggaaaccga tcttccacaa attgaagaaa
      481 aaatgaagga acttgctgct cgtaaggagc ctattattcg aaagaatata agtaaagcag
      541 atgccttaaa aatatttgaa gaaaaaggcg acgaatataa aatagaatta atacaggatc
      601 tgaccgatgg aactattact ttgtattcac aaggaaattt tactgatctt tgccgaggtc
      661 ctcatttacc tgatacagga aaaattaaag ccataaaatt attgagtgta gctggggctt
      721 attggcgagg cgatgaaaaa cgaaaacaac ttacacgaat ttatggaatt actttcccaa
      781 caaagaaaat gttggatgac tatctggtac ttcttgaaga agctaaaaaa agagatcatc
      841 gaaaaatagg aaaagaactt gaactattta tgttctccga tatggtaggg aaaggattgc
      901 ctatctggtt gcctcgtgga acagacttac gtcttcgact ggaagaaatt ttgaagaaaa
      961 ttcagcgacg ttttgactat cagcaagtga ttactccaca tattggaaac aaacaattat
     1021 atattacttc tggacattat gccaaatatg gaaaagattc atttcaacct atccatacgc
     1081 ccgaagaagg cgaagaatat ctgctaaaac ccatgaactg tcctcatcat tgcgaaattt
     1141 ataaattcaa acctcgttct tataaagatt tacctttacg aattgctgaa tttggaacag
     1201 tttaccgtta tgagcaaagt ggtgaacttc atggacttac acgcgtaaga agttttactc
     1261 aggatgatgc tcacatattt tgtcgtcccg agcaagtgaa agatgaattt ttgaaagtaa
     1321 tggatattat tttcattatt tttaaagcat tggattttaa taattttgaa gctcaaattt
     1381 ctctacgcga tccaaataat aaagaaaaat atattggtac tgatgaaaat tgggataaag
     1441 cagaaaaagc aattgtagaa gcatgtgaag aaaaaggatt gaaagcaagg gttgtgttgg
     1501 gagaagctgc tttttatgga ccgaaacttg attttatggt aaaagatgct attggtagac
     1561 gctggcaatt gggaactatt caagtggatt ataatcttcc agaacgattt gagttggaat
     1621 atacaggtga agataaccaa aaacatcgcc ctgtcatgat tcatcgagca ccttttggct
     1681 cgatggaacg ctttgttgct ttattgattg aacataccgc agggaaattt ccattatggc
     1741 ttgcgcccga tcaagttgtg atattaccca tcagtgaaaa atataatcaa tatgccttcg
     1801 aagtagaaaa aattctaaag gaaaaatcta tacgagctat agtagatgat cgtaacgaaa
     1861 aaattggccg aaaaattcga gataatgaac taaaacgaat cccttatctg ttaatcgttg
     1921 gagagaaaga acttgttgga agggaagttt cggttcgtcg tcaaggtgaa ggagacaaag
     1981 gaaccatgaa aattgaagat tttgctcaat taattcttaa tgaaattaat accttattaa
     2041 aacagtttga ttaatttcat tatgttttaa aaacaacaaa atatttgaat cagctttagt
     2101 tttttagact tttgctgaat ttattgtgta atgaatgaat aaccccgagg caagcttcga
     2161 ggaattcttt tgattaaaaa atagaaattg taacagattt cttataaact tttatttaaa
     2221 aattgcctta taaatatcat taccattggc ataaattaat aaggcaagta ataataacat
     2281 accgatggtt tgagcttgtt ccagaaattt ttcactcggt ttccttcccg taaagatttc
     2341 atatagcaga aataaaacat atccgccatc caatgcagga ataggtaaaa agttcataaa
     2401 tgccaatata actgataata atgcggtcat tgaccaaaaa atttgccaat cccacttttt
     2461 aggaaataat tttccaatgg caccaaaacc acctaattgt ttggaacctt ctttggtaaa
     2521 taccaactta aattgtttta cataacttgc caacgtttct actcctaatg aaactcctgc
     2581 tggtatagaa gccaataacc catattcgat acgtgtagtt tcaaaaaaat caatataagg
     2641 tcgaaccgca actcctaatt tccctgtttc gtctaattga attttagatt cgtgtaatga
     2701 gccatttcta taataggcta atgttattac agaatcacga tactggctca actgggaggc
     2761 tatatcttga aaaatataaa gcggtttccc attaattgct accacactgt cgcctcgctg
     2821 taggtgagct tgccaagcgg gagtatttgc gaatacactg tcaactacaa atgggaaacg
     2881 ataagaaata agactttctt ctccacttgc taatactttt tgaggaaaat ccttaggcaa
     2941 ttgaacattt acaatctgag tgtctcctct cagtacggtt acattttggg ctccatcaat
     3001 cagaattttt tcaatgacat ctgaaagctt ttcgcaattt tccccatcta tactaagaat
     3061 tttatcacca tttttgaatc catgctctaa aaatgtttct gaaaactgaa gaccatattt
     3121 tgcattttga ataggaagat attcctgccc ccatttatat aaaatacctg aataaattac
     3181 aatggctaaa ataaaattca tcaccacacc tgctgtaata attggtaaac gttgccagac
     3241 agattttgaa cgatattccc atggttgtgg ttcagctttc atctgggttt tgtccataga
     3301 ttcatcaatc ataccagcaa ttttgcaata accaccaaga ggtaaccacc caattcccca
     3361 ttcggtactt tctggatatt tacgccaatc gtcatcagga agttctgata gtggtatcgg
     3421 ctccataata ggtttcccgt tttcatccag cataatatca ccatcagcat cccttttagg
     3481 acgttcattg ggcggaacat ttttggcaaa aaacttaaat ttccatttgc cgttaatctt
     3541 ttttgctctc attatcgaga agctgggatt gaaaaatatg taaaattttt ctacgcgtac
     3601 tttgaaaatg cgagcaaaaa tgaaatgacc aaattcatga ataataacca gtatagataa
     3661 actaaaaata agttgcaatg ctcgaattaa gaatgtttcc atatttaaga aataatgatt
     3721 aactgaactt tttgaatgga tttttgattt taaaaatcgt taagttgatg tttgatgatt
     3781 ttttaatttt tcttttaaac aatacccgtg attattaaag aattatttat taatcaattc
     3841 gtaagttaat attctaactt ctttatctgt atttacataa tcttcataag tgggttgctg
     3901 aataaaaagt gcctttgaca ttattttttc aattatctca ctcatttgta aaaaaccaat
     3961 tttatttttt aagaaagcat ccactaccac ttcgttggca gcatttaaaa tgcagggcat
     4021 atttccaccc tgatgtacag cttcataagc cagtaaaaga ttccgaaagc gttccgtatc
     4081 aggtttctcg aaagttaatt gagaaatgat attaaagttg atgcgtgaaa aattggtatt
     4141 gaatcgctct ggataagtaa aagcatataa aataggtagt ttcatgtctg gaatacttaa
     4201 ttgtgctttt acactaccgt ctgtaaattg taccattgaa tgaataatag attgaggatg
     4261 aatgagaatt tcaatttgtt caggctctaa gccaaaaagc cattttgctt ctatcatctc
     4321 aaaaccttta ttcatcaaag tagcagaatc aattgttatt ttggttccca ttgtccaatt
     4381 gggatgtttt aaggcttccg ccgatgttac cttctgtaaa tctttgaacg atttagtgcg
     4441 gaaaggtcca cccgaagctg ttaaaataat tttttcaata tcgttgttac cttctcctgt
     4501 caagcattga aagatagcag aatgttcaga gtcaacaggt aaaatcatag catgatattt
     4561 ttcaacaagt tgacctatta attcaccagc tactaccaaa gtttccttgt ttgccagtgc
     4621 aattcttttc cctgcctgaa tggcatgcat agttggtttg agtgccgaat aaccaactat
     4681 tgctattaat attatatcca ctgattgaat ttctgcaatt tgtgaaattg cctcctcgcc
     4741 agcaaatact tttatcggaa gttcagctaa cgattttttt aactgttcat aatgaatagg
     4801 attggcaata gcaaccattt caggatgaaa ttttcttgcc tgttctatca acaaatcaac
     4861 gttgttattc gcagtaagag catatacttc aaacttatca gagtattgtt caatgacttg
     4921 caatgcttgt gtgccaatag agcctgtaga acctaaaata gttatttgtt ttttggtttc
     4981 gctcacgtga attaaaaatt tatactgatt tttttgattt cttaaaaaat aataatctcc
     5041 tccggattaa tgggcttacc ctgttgccac aactcaaagt aaaactcttt ttttgcttta
     5101 ttggaatgat tattttctcc cgttatagca atagtttcac ctgcttttac tacattgcca
     5161 acctttttta ataatcttgt attgttttta taaattgaca cataattatt ttcatgttgc
     5221 acctgaatga cccagtcaaa atcaaatgta aatccagcaa aaattactgt gccatttaat
     5281 acactcatta cactttgatt aggtggagtt aaaatattaa ttccatagcg gtgattctgt
     5341 aagctgtaag aagaagttat taccccttgc acaggtcgaa aaaaaacata agtattttca
     5401 ttcttattga tactgatatt tgctaaattg aatttttctt cttcttcaaa ttttttacag
     5461 aattcttcct ctttttttga tttttcaagt aattcttgag attttttact tagggtaact
     5521 gaatcaagcg aagagataga atcaggttga atctcttcgt ttattatttt tttaataagc
     5581 tctatataag ctgattgttg gctgactact ttcattaagg aatcagtttc caatgattct
     5641 tgaattattt ttgctcgatc gcctgtttct ccataacctg gaagataata tcgcaaaggg
     5701 gttacataaa taagaattgt taataaaaca aaagtcagta cagtgaaaag gaaaaaataa
     5761 agaataacag taaatcgcga caaatgaata tgccacgatt cttccaaagt attttcattt
     5821 acaacagaaa cacgatattt aaaacgcagt ttttgtaata ataatgaaat ttttcctttt
     5881 ttctttttca ttcaatgatt ctgaatacaa aatgctttac aaaattagca tatttcgaag
     5941 gaaaataaaa atcaagaagt tcaaagtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnngca tataattctg ggaaagctgc taaaataaga gttctaccaa atacatcgta
     6121 acctgaaccg atactagcaa taccatttgc acaaacttgt ccttgccgaa ttgctgaaag
     6181 taatcctgta agagctaatg ctaaacttcc tccaaataca gcggcagcgg caccccatgt
     6241 catatcaggc accaaatgag gagacaataa aaaataaccc aaaaaaccat ataaaccttg
     6301 tgtaccagga atagcgctta aaaccatata attaccaaaa gcatcaggat tcttttttaa
     6361 agcacctaat gtggcattcc cagcaattgt tactccaaca gcacttccta cccctgaaag
     6421 tcccaccatt aaaccaattc caatataagc taaaataata ggtcccataa tttatttttt
     6481 taattgttaa tgttttatat attttgattt tttattcttt aaataataga tttttcttct
     6541 ttataatgtg caaatggttt atattttttc cctccacctt caaaacctgc atttttataa
     6601 aattccacaa aagtcaaacg cataggatga acaaaagcac ctaagccaga catgaatata
     6661 ttaatactgt gtcctatgag cataattatt atcataacaa tgggtccaaa aaaaggaata
     6721 cctttgctga tattaattgc caagtcattg aatacaaatc ccattactgc tcctgaaatg
     6781 cccaatgcaa acaatcgaat ataagacaaa gtatctccca ataagccagt agccatatta
     6841 taagtatccc ataaaccaga accaatgttt ataagaggat ttttatgaat attgttcaac
     6901 aagaaaacaa atattacacc aattccaccg aaaacgatat ataaccattt tgctattgta
     6961 aaaggaagac cagcataaga atttaaaaca aaagtgcctc cacaacctat taaaatagat
     7021 aaccaacccc atgttgaaaa agatgctggc caaccataac gtaagacttt cccaactgct
     7081 ttaataatca ttccaaataa gatttgaata acacccagaa ttaaagaaat ataaaacaat
     7141 tgatttgaat caatcatgat tttttttaac ttctctaacc atggtatttc ggcttctatt
     7201 aaattgaagc caaaaaatgt gcctgttatt attcccataa ttacagtaga aattcctaaa
     7261 caaatcatca aggaaagtac aggttttatc gcatcattga ctttttttcg aaggaaaatt
     7321 gcaaataaaa ctaaaagtaa accataagca gcatctccta aacatattcc gaaaaagagc
     7381 atataaaatg gagcaaagaa aggagttaaa tcaatttcat aataattagg taaatcatac
     7441 aaattgccaa taaactcaaa tattcgagca aaacgattat tttttaactt tacagggact
     7501 ttttcttctc caacaggttc gcttgtttca taataaattt cagaatcatt taagaaattt
     7561 tgtaattcct cttccgaatc ttccggacac caaccttcaa tcaacataac tttctcttca
     7621 atagtaggtt cagtattaag cacaaccttt gtaaaatcaa ttgtttcaaa agtttcaact
     7681 tgaaaagatt ttaaagtatt ataatcttca accgccattt ttattagttg ttgctttacc
     7741 tcctcaattt ctttccttat ttctgaaatt tgcaaatcta actctggagc agttctatcc
     7801 gacaatttaa taggatcggc atctatatca acaacttcgc caggtcgagt aatggtaata
     7861 aaataacggg ttgaacctac ggtatcaatt tcaaaggcat tataaattac ttcccattct
     7921 tgatcaaact tgcgaatatt acaactaaaa aaattgatga tataacccgc ctcacgtaat
     7981 tgatgcagcc tttcaaagct aaaaattccc caaacttcca ttcggtctct ttctttctca
     8041 atttgctgaa gtttttgttc aagtgattct ttttgagcta atttattttc tatgtttatc
     8101 agcacttcta agccatcttt aacaggatcc gctggcaatg gtattatttc ctttgaaagt
     8161 cttttttcca actgtttcaa cgcaattttt atgcgagctg ataacagcat cttttctcgc
     8221 aaggcatcgt tttcaggtat tccttcaggt ttttctatca cgtggagcac accaatctcg
     8281 cggatttttt ctaaaaattc tatatattgt ttgtggtata ccaaaaaggt atattttgtc
     8341 attttaacaa tcatatattc ctcccttcta tcttttcatg acgtgatttg ataatttttt
     8401 gggaagattt cgacagattt tcctcatctt ccataaatcg ttttattttt cgaatcgcat
     8461 cttcataacc gggtatttgt actttctcaa ataaatttac tttttgagtc gtcttttttc
     8521 tttcatgctc caacaagtta agcttttgaa tataaaactc atgctcaata gcaatttgtg
     8581 ctaattcttt taataaggct aacccatcta aataccattt aggcgcatta aataagctaa
     8641 aagatttggt tttataaact acctcatcta aaactgggat acgtactgca gcaatctttt
     8701 ttatactcat tttaatatct tccacttcga gcaagctggt gtcaaattcg ccccacaaag
     8761 ccaacatttt atcataagca gcaatacgag cttcgaattc agcatttatt ttttttatat
     8821 catcttttgc ttttttgact tctgtacgca aagcactttc tttattttta attgtaggta
     8881 atgctctgac tctgatcttg agattttttt caagattttg taacgaagtt ttattatatt
     8941 gaaactgtat tgccattaaa aaaaggaatt tattttaatt tttataaaaa atttatgaat
     9001 taatttctcc aatatttatc cactaaatct tgtttaatat taacctcact tggtttaaag
     9061 tatttagcaa acaatttcca ggtgacatcc aacatggtag tagtatcaat atttacatca
     9121 atagctaata ttttttctga atattcttta gcgaaagcca atgcacgttc atcgtaatcg
     9181 gtcaaatcaa atccattttc aagttttgtt ttggcattgg cggcatcagc ataaagacgt
     9241 acggcggcat tcattacttg tggatgatct tcacgnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnaaaga acccggcatg gaatcttttg atggaatttg atccatacga
     9421 ttcgaaacaa ttgctaaggc atcggcatac aaagtcatat ctgtaagtag taccaatact
     9481 ttttcgtttt tatctactgc aaaatattcc gcagcagtca aagccatatc cggaattaat
     9541 aaacgttcca caggtggatc ttctgtagta tttataaaac ttataatccg atctaaagcc
     9601 cctgcttcgt taaaaacatt tttaaaataa agatagtcat cgttagttaa tcctatacca
     9661 cctaaaatta ttttatcagt ttgagcacgc aaggcaacat tagccataac ttcattataa
     9721 ggctggtcag gatcggcaaa aaagggaatt ttctgtcctg aaaccaacgt gttattcaaa
     9781 tcaatacctg caattcctgt tgcaattaat tcagacggtt gttctcgtcg gacaggattg
     9841 acggtaggtc cacctatttc acgttcttca ccttcgattt caggtccacc gtcgataact
     9901 tcaccaaaag cattaaaaaa acgtcctgct aactgttcac ttactttcaa ggttggggct
     9961 tttcctaaaa aaataacttc ggcatttgta ggaatacctt ctgtgccttc aaaaacctgt
    10021 aaagtaactt catctccaat aattttaact acttgagcca acttaccatt aacagtagct
    10081 aattcatcat tgcctacatt agtagcatga agtgaacatg tggcttttgt aatctgacta
    10141 atttgtgtat aaattttttg aaatgctttt gttgccatct ttatgattaa ataataaaaa
    10201 attctactta aataaattta ttaaaattgc atcaattact tgaatttact atttttcttt
    10261 cctccaaaat tttattcaat tctttttcat atttttcaaa atcttctgaa tgaaaaacag
    10321 aataattcat ttgtttgcag atattaataa ttcttttgaa ataatccatc acttcaacaa
    10381 aattactgaa tttaaaatcg gcgttacaaa tattcattat taaacttaac atataatgtt
    10441 gtcgttcaat aggcgttaat gcgtcaattt catcaaaagc atcctgctga agaatcacaa
    10501 aatcaattaa ttctgatttc caaaaagtaa tatgataatc cactggtacc ccgtcgtctc
    10561 ctaagatatt aatttgttct tgcacttctc ttcctcgttg aagaagggtt cgcatatcat
    10621 tgaccatatt tgtccaatcg ggcgaaatgt gctcagttac atattcttca aattcaggat
    10681 aatcaaaata tttagaatag ctttcaatgg gattaatagc tggatatcgt ttactatctg
    10741 cgcgtgcttg ttcaagagca aaataacagc gagctacttt tttagtgctt tcagttaccg
    10801 gctcttttaa atttcctccc gcaggcgaaa ctgttccaat aaaagtaata gaaccggttt
    10861 taccattttt taattgaaca taccctgcgc ggctgtaaaa attagcaata atagcagata
    10921 aatccatagg aaaagcatct tgcccaggta attcttccat ccgattactc atctcacgca
    10981 aagcctgagc ccaccgtgaa gtagaatctg ccatcaataa aactcttaat cccattgccc
    11041 gataatactc agcaatggtc atggctgtgt aaacagaagc ttcacgagca gcaaccggca
    11101 tattagaagt atttgctaca ataatggttc tttccattaa tagtctgtcg gtatgtggat
    11161 cggttaattt tgggaattct acaaaaagtt caacaacttc attggctcgt tcaccacaag
    11221 ctgccataat aacaatgtct gcttctgctt gtttggaaat ggcatgttgt aaaacggttt
    11281 ttcctgtacc aaaagctcct ggaataaaac cagttccacc ttcaacaatg ggattagctg
    11341 tatcaattac acgtacgcct gtttccaata gtttaaaagg acgtggtttt tcggtatgaa
    11401 tattaattgc cttttttact ggccattttt gtgtcatatt tacctcaatc tctttcccag
    11461 cttcatttat caatactgca atagtatcta aaattttata atcaccagtt ggaacaatag
    11521 ttttaactgt atattttcct tcaaatgcaa aaggaaccat aattttatga ggttgatgat
    11581 tttcatctac ttcgcctaac cagctacctg cttccacctc atcaccaggt tttgctaatg
    11641 gtttgaaatt ccataatttt tctgtatcaa gtggaaaatt atattcccca cgttgaagaa
    11701 aaattcctga aaacttattt aaatcattct gaagtccatc aaaatttttc gaaagtaaac
    11761 ccggtgccaa cgtcacttcc agcatatgtt cttcaaaatt aacattgttc cccactttta
    11821 aacctcgtgt actttcaaat acttgaacaa aggcattggt atctattact tttagcactt
    11881 cggccattaa cattttaccg tctaattcaa tgtaacatat ttcattttga gctactggtc
    11941 cgtcaatttc caccgttacc aaattagaaa ccacaccttt tacttttcct tttgttgaca
    12001 taattttttt aatacagctt ttaacaaaat taattatttt gacactattc tatttagtca
    12061 tttctattgt ttttttacat tatctaaaat atcttcttga cgttacattt gcttcttctt
    12121 cgaaattaac actttcttta agatgattta ccattgttct aaaaatttgt gttccttttt
    12181 ctaccgacaa aggctgccaa cgttccagta tttccatctt aattacaaaa gcaagtattt
    12241 tttcaacaga aaaataatga aaaaatgtat tctcttctaa ccatttccac ttaaaagcat
    12301 ctatttcttt ttcgcgttgc aataaatctt tttcttctgc tatttgcaaa agtgtctcca
    12361 aattctcaaa aatattggtc aaaccaaaat cacgagcatt agtagtacga agaatttgtg
    12421 caatttcatt atttcctaca atcatttttt tttggtcaat accatatttt cgacaagcta
    12481 cagccaccaa gatattgtta atattcaaat taaattcaaa ccatgatttc aaaaaatcat
    12541 tgttattatt cattgcatat tcataatata aagtagaaag ataatcttct tttgaaatgc
    12601 cttcggacca aaattcttct tcataataaa cagcataaaa ttgctgaata taaggtaata
    12661 atcttttatc gttgggatgt tcagtttctt tcatcaaagt tatgagttgc tgccagtctt
    12721 cagaagttaa atttcccagc ggatttaatt cagcttcttt atttgccaag tatagaagaa
    12781 aattttgatt atcatatttg gcaaaaagta gttttatcag ttcaacatca gaaggtgcca
    12841 gttggactaa taattctttt ttaaaatcaa gaagaggaag aaatagtttt gtgtcttcta
    12901 tatgtaaatc aggaagtcct gcaattaaat aataataatt catttttctc ttttcttgtt
    12961 cttcaaaccc tcaatgcttt ttctatcaat taaattcaaa ctccttgttt caagcagcta
    13021 attacatttt ttcaaaaagc atatcgacca gttttggtcg tagaaaatct ttaaaatacg
    13081 caataaattc ttcttcgccg aaggtaattt tgtaacctgc cccttcagga acaatagtaa
    13141 aatcagcctt agcgttattc acttttgtta tttttacacc tttattcagt aaatctttgg
    13201 catttgccat aaaataatct gttaaagctt tctcatcttt ggtttcaata gtaatagctt
    13261 gattttttgc ccactctttg accaatgtca aaatcacttt ctgcataaaa tctttgtcag
    13321 cagtagctgc ttttacagaa tttgaaacaa tttcaccaca aattaaattg gcaatttctg
    13381 attttaaggc attcaccgct tgttgagcaa agaggcgtaa ttcagcctgg gtgtttttgg
    13441 ctatgttggc tgccttcgtt tctgcttccg aaataatttg cgctgcctct tcatgggctt
    13501 ttttaattat ctcatcagct tctgctcggg ctttttcaat aatggctttc gcttcggcat
    13561 ttcctttttc tactccctct tgataaattt tgtcagttaa ttcttgtaat ctcgtattca
    13621 taagaattta tttaaaataa cattatgcaa atttaatgtt ttatttttag tagattattt
    13681 ttttataatt atttaaaaaa attatacacc ctaaaagatt aagaaattta gtaacataca
    13741 aattattcct tgattttcaa atataacttt tttgtgtaac tttttatgat aactcatttt
    13801 ttattagctt tgtacgtatt aattgtcaga tgtaaatcaa aatagaattt tacttttatt
    13861 tttgaaaaac taataaatta atgagatgga tattttactt attattttat caggtatttt
    13921 cgttttttta ggttttattg gttgtatttt acctattttg cccagtgttc cgctaagcta
    13981 tataggcatt ttactgttgc attttacatc aaaaattcaa ttttctaccc aatttcttgt
    14041 catttgggga ataattgtgc ttttatccca attgctggat tattttgtac cgattttggg
    14101 aactaaaaaa tttgggggta gtaaaaaagg tatatggggc agcatgttgg gaatggtaat
    14161 aggattattt tttgcaccgt ggggaattat tataggtcct ttcgttgggg caattactgg
    14221 tgaacttata gatggtaaaa caaatgttca agccattcgt gcaggattgg gttcatttat
    14281 aggatggata attaatacag cttgtaaact gataattgct ggatttttta tctattatta
    14341 ttttgaagca cttattaagg tatattaaga tttaatttat cagacaactg tttttttaat
    14401 tcatcaaaag taactaactg ttgattgcca gttaacatat ttttcaacat tactttttcg
    14461 gaaaccactt cattttcgcc gacaatagca acaaaaggaa tatttttatt atttgcataa
    14521 tccaattgct ttttgatttt agcaggttca ggataaattt caacgttgaa tccaaaactc
    14581 cgtaaccttt gcaaccacga aaaactatat atcaattcct tttcaccaag tgcagtaaat
    14641 aaaatttggg tttgcgattg taaggtttgt ggataaagtt gaagttgatt aagcacatca
    14701 taaattctat ctgctccaaa agaaattcct actccagaaa ctcctttcat cccaaaaaca
    14761 cctgtaagat cgtcataacg accaccacct ccaatgcttc ctattttaac atctaaagct
    14821 tttatttcaa aaatagctcc tgtgtaataa ttcaaccctc gagccaaagt caaatcaagt
    14881 tctggaaaaa atttgatgtc cacattgcta caaagccgaa aaatggtttc aatttccgaa
    14941 attcctttta gaccaatttc agaggaaacc agtattaatt tcaattggtc gagtttttct
    15001 gcattattgc cttttaaagt taaaatgggt tgcaatttag ctactgcctc aggcgaaata
    15061 cctttttcaa tcaattcttc attaacattt tgcaatccaa ttttttccat tttatcaacg
    15121 gcaatagtaa tatccaaaat tttttgtgaa gcacctatta tttcagctat tcctgcaaga
    15181 atttttcgat tattgatttt aattgtaaca ttaattttta attgttcgaa aacttcattg
    15241 acaatttgaa caagttctaa ctcattaacc agtgaatcac ttcccactac atcggcatca
    15301 cattgataaa attcacgata acgccctttc tgaggacgat cagctctcca tacaggttga
    15361 atttgatacc gtttaaaagg aaaagtgatt ttgtcgcggt tttgtaccac aaatcgggca
    15421 aaaggtacag ttaaatcata acgtaaacct ttttcacaaa ttttattgga gagctttaca
    15481 gtatcatgtt ttaataactc attatcagaa atatctgcaa gaaaatttcc tgaatttaaa
    15541 accttaaaca gcaatttatc tccttcttct ccatatttgc ccagtaaagt agacaagttt
    15601 tccatcgcag gcgtttctat ttgttgataa ccatatcggc aaaaaacatt tttaatggta
    15661 tcaaaaatat aattccttct tgccatttct tcaggcatga aatcacgcat cccttttgga
    15721 attgtcggtt tttgcataaa attttttaaa ataataagat agatgtcctt tttaaaatgg
    15781 ttcctacttt ttgttaaaaa ctacgagaat attaagtgat atattttttc tgtagctcct
    15841 acatttttta aaatataagc acctgccctt ttacctgctt cttcatcgtc gagcagttca
    15901 tcaaattttt tttcaagtga atcataatct gaaatagaaa aagctcctcc ttctgcaatt
    15961 aattcatgtg cttcgcgaaa tttttgataa tttggaccaa aaactaccgg gatattccat
    16021 acagcagctt ccaaaatatt gtgaatacca gctccaaaac caccaccaac ataagcaact
    16081 ttgccgtatt ggtaaataga agataaaaat cctatagcgt ccaccacaag gcaattaaaa
    16141 gtatgaatat ttatttctgt agcttcagtg tatcgtatat atttcccctc taataattta
    16201 aaaatatcat ataaatgact tttatcaatt tcgtgaggca ccaaaataag ctttatttgt
    16261 ggatgaagtt tgatataacg tatcaataat tcttcatctt cgggccaggt gctgcctgct
    16321 acaattactt tactgttgcg ggcaaaagat tcaatcaaag gtattttttt cggttccaaa
    16381 gataaagcat ataccctgtc aaaacgcgta tcaccgcaca ctgtaacttt ctgaataccg
    16441 tgctcgttaa gtaattttgc tgataaatta tcctggacaa aaatatgaga aaatgtattc
    16501 aacagttgaa gataccactt tccatatttt ttaaaaaaaa tctgattggg gcggaaaata
    16561 gccgagatgc aatatacagg aattttttca gcttccattt caagtaaaaa attaggccag
    16621 aattcatatt tgataaaaat tgccttcgta ggcttgacaa ttttcaaaaa ttcctgtgca
    16681 gcttttctgg tatctaaagg gagataggaa accacgtcca cgcaactata atttttcctc
    16741 acattataac ctgatggaga ataaaaagtc aataagattt tagtttctgg atgttctttt
    16801 ttcaactttt caatgatggg tcgcccttgc tcaaattctc ctaatgaagc cgcatgaaac
    16861 catacatacg aagattgaga atctattttt tctctcagta cttttaaggc atttcgttgt
    16921 ccacgataaa gctctctcgc tttgtcataa aacaacgaag caataaaaat taaaatacca
    16981 taaacatgta taccgaaaga atataaaatt ctcattctca aaaatattta aaaatgaaat
    17041 aaaaaagatt gataaatatg tataaatatt tattgtcgaa ttttatacca ccttattgta
    17101 aaaaaataaa agtatgaaaa acaaaaggca tctcaaaaat agttttgaaa cgcctttttt
    17161 gaaacactta aaagtaaaat tactttctta tacccaactc ctgaattatt gcccgataac
    17221 ggttaatgtc cttttctttt aaataatcga gcaaacggcg acgttttcct accagtttta
    17281 caagagcgcg ttctgtacta taatctttct tatttgactt taaatgttga gtcaaatgat
    17341 caatacggta tgaaaacaat gctatctggc attctggtga accagtatca gtgttagact
    17401 tcccgtactt ttcgaagatt tcttttttct tttctgctgt taaatacata tttaaaatta
    17461 aaaattaatt atgagcctgc aaatttacaa attatatttt aattccctaa cttttcttga
    17521 aaaaacttcc acaaaatttt atttaataga cttttacaca agaactgaca ttttaaaaaa
    17581 ctgacataag gtagttcaaa acaacaacaa aaacttccgt gaaaagccat gagatagatt
    17641 tttaaggaaa attttcatct caaatttaaa aattgagtgc aaaaatattc aatattttct
    17701 taataaagac ctaaaatttg caagtaaaaa attgtttgag aaatttattt ttttcaaata
    17761 aactttgaaa aactctcttt taaaaaattg attcggttaa tgtgaattaa gaaaatggaa
    17821 atacaattat ccatttttga ttagagttac cctatttgta gattacaaaa aaatgcctaa
    17881 ttttgtaatc tcaaaaataa gaagaaactg ctaaaataag acaaaatgaa gaggacattt
    17941 caaccatcgc aaagaaaaag aagaaataaa catggctttc gtgaaagaat ggcaactgcc
    18001 aatggccgaa gagttttggc tgcccgccgt gctaaaggaa gaaaaaagtt aacagttgcc
    18061 gatgaagggc gtcataaaag ataaatttat tccctgagtt tgtgaaataa ataataaagt
    18121 aaaaactggc ggaaggtttt tacttttttt atttttagca tttcgatgac tgaaataatt
    18181 tcattatttt tgacgttaac ttgataaact tacatttttt gaaagtaagt ataatatttt
    18241 tatgaacttt aaaaaatttt ggaaagaaac ttttgctggc tttgtggtaa agaatgtatt
    18301 gcttgccatt gtggtgttta ttttactctc atgggtgacc cttatttgta ttgattttta
    18361 tacccatcat ggtgaagctg aaattattcc cgatttaaga ggtgcttatg tagaagaagc
    18421 tgaaatatta ttaagcaaaa aaaatcttta tccgaaagta atagattcag tatatattcg
    18481 agataaaaaa cttggaacta tcgttgaaca aattcctcca cctaattcta ctattaaacg
    18541 aaaccgtcca gtttatttaa ttattaattc tcgtcaggtg aaacaaatac ctttgcctaa
    18601 tgtcattgat gtttcttatc gtcaagctat tgcaatgctt tcatcggttg gcttatcagt
    18661 tgagggagtt caatatttac catctgaata taaagatctt gttcttgatg taaaatataa
    18721 agggagtagt cttttgccgg gtacacgtat tccagaaagt agttctgtaa tcttagtagt
    18781 aggtagtggt tatggtgata gtttatcagt cgttcctcaa ttaaaaggat taacattgca
    18841 agaagcaaaa gaaaaagctt ttttgaattc ttttgtaata ggatcgactt tatacgatat
    18901 acttccaaat ggaaatgaca atgattattt tatctatcgc cagcgtcctt ccgcaggaga
    18961 attagcaaat gttggtagca gaattgatgt atggttgact acttctaaaa aacgcttaaa
    19021 agaaactttt gatgaaaatg ataattcatt ggatagtaat ggtgaggatg aaatattttt
    19081 ctgaaatttg tagactataa tgttgatgat tcgccaaatc aacaatcttt tcttaaatcc
    19141 aatctatttt taaaaaatca acaaaataac aaactgaaaa ttgttagaag taactaaatg
    19201 ggaagaatta attgaatctg ctgaagaatt agaattttat gaacattacc gatttaaagt
    19261 agataaaaga cagtcaccat tacgaattga taaatatctg gttaattgca tagcgaatat
    19321 ttcccgtaat cggattcagg aagctgctga aaatgggaat attttggtaa atgaaaaacc
    19381 tgtaaaatcc aattataagg taaagccagg agatatcatt actattgtga tgatttatcc
    19441 cccgtcccat tttgaaatta ttcctcaaaa tatccctctt aatattgttt atgaagatga
    19501 agaattgata gtggtaaaca agcaaccagg cttagtagtt catcctggca taggcaacta
    19561 tgatgggaca ctacttaatg ccttagctta tcactttaaa aatcaacagg atattgattt
    19621 taatgatcct cgcattggtt tggttcatcg aattgataaa gatacatctg gtttgctctt
    19681 aattgcaaaa acagaagatg caaaaacaca gcttggaaaa caattttacg ataaaacaac
    19741 cgaacgccga tatcaagcac tggtatgggg aaacatcaaa gaagaggaag ggactattga
    19801 aggatcactt gcccgtgatc cacgaaacag aatgttgttc tcggtttttt cagaagaaga
    19861 aaattcatca gctaaaaggg cagtaactca ttataaagta ctggaacgcc tggcttatgt
    19921 tacattagtt gaatgcaagc tggagacagg acgaacgcat caaatacgtg ttcacatgaa
    19981 acatattgga catccagtct ttaatgatga acgctatgga ggaaatgaaa ttttaagagg
    20041 aaatccttcg actagctatc gtcaatttgt aaaaaattgc tttgaaattt gtcctcgtca
    20101 ggctttacat gccaaaactt taggatttat tcatcctaaa acaggagaat ttatgaattt
    20161 tgatagtgaa ttgcctgctg acatggaaca gttgctggaa aaatggagaa attatgcaaa
    20221 aacaatctaa ttgattcatt ttattaattc caacatcaat attaaaaaac caactatcaa
    20281 gaaaaatcaa catagtatct tctcgatgcg gcgttgatgt cttccacctt caaaatcagt
    20341 tgttaaaaaa atatcaatca ttttttttgc ttcttcttga gaaataaaat gagcaggtaa
    20401 agaaattaca ttggcatcat tgtgctttcg agctaagaaa gcaatttctg acatccagca
    20461 aagagcacca cgtattcctt tatgtttatt tactgttata ttaacaccat taccacttgc
    20521 acaaagggca ataccaaaat caaagtcacc attttctacc gcagttgcca atggatgggc
    20581 ataatcagga taatcgcagc tttctgcact gtaagtacca aaatctttta aagcagccaa
    20641 attttttatt ttcaaatatt caatcagctc attttttaat tcataacctg catgatcgct
    20701 acaaatggct atttttaatt ctgtcaatgg tttcataatt aattttattt atattcagat
    20761 atttaaaaaa tcaaacaaat aattctacat cttctttggt aattttagat cctcctaaaa
    20821 taataagacg ttcaacaata ttccgaagtt cacgaacatt accgggccaa tttctttgct
    20881 gcaataaaga tacagcctct ggttcaaact ctttgacctg tcttccctgt tcataacaaa
    20941 tcagttcact aaaatgagta atcaagagtg gaatatcttc ttttctttct tccagtgaag
    21001 gaacatgaat aggaataaca tttaaacgat gaaataaatc ttcgcgaaaa ttaccctttt
    21061 caatttcgtt tatcagattt ttgttcgtag ctgcaataac tctcacgttt accttgatga
    21121 ttttatcgcc tcctactcga gtaaattcgt tttcctgcaa aattcggagc acttttgtct
    21181 gagctgataa actcatatct ccaatttcgt ctaaaaaaat agtaccttca tcggcttgtt
    21241 caaattttcc aattctttgt ttgagagctg aagtaaaagc tcccttttcg tgaccaaata
    21301 attcactttc aattaattca gaaggaattg ctgcacaatt tacttcaata aaaggggcgt
    21361 ttgctcgatt gcttttttga tgcagttgcc tggcaaccaa ttcttttcct gtcccattat
    21421 caccagtgat taaaactcgt gcatcagttg gagcaacacg atcaatcagc cttcttactt
    21481 tttctatcgc aggcgattga ccgataatct ggtatttctt ttctgtattt ttctttaatg
    21541 ttttcgattc tgttataagt tcttttcgct ctaaagcatt acgcactgtg attaataaac
    21601 gattcaaatc aataggcttt tctataaaat caaatgcgcc ttttttgata cattctactg
    21661 cggtatcaat agtgccatgc ccagaaatca ttacaatggg aacttcaatt tccctttctt
    21721 tcaaaagcgt taacaattca atgccatcta tctctggcat ttttatatca gaaaaaatca
    21781 agtcaaatgc ttcattttga gcaatttcta aaccttgttt gccattttca gccaaagata
    21841 ctttatgttt ttcaaattcg agaatatctt ttagtgtatt acggatactg cgttcatcat
    21901 caattactaa tatttttgcc atgattttat cctattccat tgtgtttatt ttaaacaaat
    21961 tgaaattcaa aatttttaaa aacttaaaaa taagaatttt ttacaacata ataacattaa
    22021 aatgcatcta atgaagttgc aattgaaaat tgatacgaat aatttcctac ttgaaaagta
    22081 ttgaccccaa acccgacctg aaatttatat aattttacgc ctcctccaaa agaaaaacct
    22141 gccatacttt tgaaatcttc aattttcatt tccttgctct gtcgataatt gtaagcagct
    22201 attaggtaaa aattattcga aggaataaat tctaaaccaa caataaaatg acgaaaaagg
    22261 ttatcaaaaa aatctatttt tttcgttcca gtggaagaaa gatactccat atcccaattt
    22321 tgcaagttat gtaaagtgaa tgaaaaacgc aacggcgcat gagctaattt cttagtggct
    22381 cccaattgta tatcgaaagg taaagcttcg taatgttgtc cttcttcgtc actgtaataa
    22441 ccttttaatt gtgtaccaac atttcgtaaa accaaaccaa gagaaaacag attatttata
    22501 tcattataac ttatgcctgc atctaacgcc agcccaaaac ttgaatacct ctcatatacg
    22561 gaataaactg gcttaaaagt tcctcctact gtaatttttt catttaacgg ataagcatac
    22621 atgatataaa aagccatatc ttttgctgta aattcgccaa ttatttcatt ttgttcggta
    22681 gtttctttaa aagttccata atccacatac tggatcccat aggctgtata atgcttgtct
    22741 ttaaaatttt ccccaaaaat aacactacca aactgaatat cagaaagata gtttgcataa
    22801 tttaacgcta ttgaatgatg agtgttggct gttagtaaag ctggatttcg aaaagcaaaa
    22861 ttcaagtcat tgtctctgat agaaacattg gtgccgccta aaccagctat tcgagaagaa
    22921 acaggtaaat ctaaaaactt ataaacacct tgtcctgctt gagaatatat taagatattg
    22981 atatttagcc aaaaaagtag agataaactt attttccttt tcattcaaga aatattacaa
    23041 gaaatattaa tactaaaaaa ataaaattaa gctgctctta aaaaattttt aaaggta
//
LOCUS       DAOSAS010000008        22468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_1655_length_22468_cov_13.093740, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000008 DAOSAS010000000
VERSION     DAOSAS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 22468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22468
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1655_length_22468_cov_13.093740"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..893)
                     /locus_tag="PLR72_00835"
     CDS             complement(126..893)
                     /locus_tag="PLR72_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOK35790.1"
                     /translation="MQLQLKKPLAFLDLETTGTNIVTDRIVEISYYKVYPNGREETKT
                     IRINPEMHIPEKATAIHGITDEDVKNCPTFKEVAKEIARDLEGCDLAGYNSNKFDIPL
                     LAEEFIRADVDIDLMKHKFIDVQVIFHKMEQRTLSAAYKFYCDKDLMNAHGAEADAKA
                     TYEILKAQLDRYSELKNDVDFLSDFTTQTKNVDFAGRIIYNENGEEIINFGKYKGQKV
                     TDVLKKDIGYYGWIMNGDFSLHTKKILTAIKLREFNK"
     gene            complement(1004..2137)
                     /gene="dnaN"
                     /locus_tag="PLR72_00840"
     CDS             complement(1004..2137)
                     /gene="dnaN"
                     /locus_tag="PLR72_00840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOK35791.1"
                     /translation="MEFVVSSTELLNRLQTISRVINPKNALQILDNFLFELKGNTLTV
                     VATDIETTLITSLEVINAKGSGRVAITSRLLLDTLREFSEQPLTFSINDTNLSLTILS
                     ANGTYNFIGQNADEYPRIPEIDEENSNLVVPGVNLFSGISKTLFASVDDDFRPVMSGI
                     YFDIVPEGLTIVATDAHKLVRFKTIYTSASLKEEAMSFILPKKPANLLKNILPKESGD
                     VLIKFDEKRVLFQLSNYTLIGLQIEGRYPNYNAVIPTSNPYKVIIDRVNFLNAIKRVS
                     IFSNQASNLIKLVFENQQVHISAQDIDFSISAEEVIPCQFEGETFTIGFKATFLIEIL
                     SSLDSSEVIIELSDPTHGALFFPFEKKENEEVVMLLMPMLLND"
     gene            complement(2252..3232)
                     /locus_tag="PLR72_00845"
     CDS             complement(2252..3232)
                     /locus_tag="PLR72_00845"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOK35792.1"
                     /translation="MNNNFFITSIDISHSQKEITYQDNIITLGSCFSENIGQKLIENC
                     FSVEMNPFGVLYNPFSISKSLEMLLEKKQFTQNDIFQYRSLWHSFSHSSYFSDISSEK
                     CLMKIQTRLDAASEFLRHANFLLITFGTAWIYEERKSGRIVANCHKLPSSAFLRRRLT
                     VEEIVANYDDLIKKLQSLFPQLYIIFSISPIRYWKDGAYENNVSKSILFLAIDALQKK
                     FENVTYFSAYEILMDELRDYRFYDEDMIHPSAFAIKYIWKKFSDTYFSEITQKIVQRV
                     EQLLNDFKHKPMHPESEEYKEFLLHIQHRKEQLLSDFPFLAERLQKSLLL"
     gene            3588..4094
                     /locus_tag="PLR72_00850"
     CDS             3588..4094
                     /locus_tag="PLR72_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOK35793.1"
                     /translation="MKNVQFEKELLALQSNMRNFAFSLTMNRDDAEDLLQDTTLKVLN
                     NKDKFTENINFKGWVLTIMKNIFINNYRKIVRNQTVIDKTEDLHHLNLPQNSGFESPE
                     GSYSIREINNNIDAFPDEYRIPFSMHLQGYKYEEIAQTMNLPLGTVKSRIFLARKRLQ
                     EQLKDYRD"
     gene            4197..4562
                     /locus_tag="PLR72_00855"
     CDS             4197..4562
                     /locus_tag="PLR72_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOK35794.1"
                     /translation="MNTAIVMLGSNFNADENIDLAKEKLAEYFEIASFSSRIVTDPEG
                     DYPYKFYNEAVKLFSDETAKETINILKQIEKELGRTPESKQTKLIPIDIDLIFWNETL
                     IKDDYYRYSFVKKCIDEIK"
     gene            4609..5019
                     /locus_tag="PLR72_00860"
     CDS             4609..5019
                     /locus_tag="PLR72_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOK35795.1"
                     /translation="MIYQKEITLVPHSKGFHLITSEIINNLPPLPQEGLLHLFLKHTS
                     AALTINENADPDVQSDLKKIFDHLVPAHQPYYDHTLEGDDDMPAHAKSTLAGVSLLIP
                     ISNSRLNLGIWQGIYLCEFRQYGGKRKIVATIIS"
     gene            5204..6676
                     /gene="proS"
                     /locus_tag="PLR72_00865"
     CDS             5204..6676
                     /gene="proS"
                     /locus_tag="PLR72_00865"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOK35796.1"
                     /translation="MANSLISRSENYSQWYNDLVVKADLAENSAVRGCMVIKPYGYAI
                     WEKIQSELDRRFKETGHVNAYFPLFIPKSFLSKEAEHFEGFAKECAVVTHHRLKTDPN
                     GNGLIVDPDAKLEEELIVRPTSETIIWNTYKNWIHSYRDLPLLINQWANVVRWEMRTR
                     LFLRTSEFLWQEGHTAHATEKEAIEEAQKILQLYTDFTEQFMSIPVIKGVKSANERFA
                     GAVETFCIEAMMQDGKALQMGTSHFLGQNFAKAFDVTFVDNNNQLQYVWATSWGVSTR
                     LMGALIMTHSDDNGLVLPPNLAPFQVIIVPIYKNEKQLADISFVADKIMADLKKAGIS
                     VKYDNDDTKKPGWKFAEYELKGVPIRLAMGPRDIENHTVEIARRDTLTKETISMENVV
                     NEISQQLKNIQKNLYERALTFRNANMKEINSYKEFKEKIEKGGFLLCHWDGTSETEEK
                     IKEETKATIRCIPFDLDKTPGKCMVTGKPSAQRVVFARAY"
     assembly_gap    6699..6798
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6799..8021)
                     /gene="serS"
                     /locus_tag="PLR72_00870"
     CDS             complement(<6799..8021)
                     /gene="serS"
                     /locus_tag="PLR72_00870"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOK35797.1"
                     /translation="MLTLKYIIENTEDVIRRLEKKHFDAREIVSQVIELDNQRKKTQF
                     ESDQAAAEINKLSKEIGVFIKQGKQEEVNALKDRTIKLKESIKSLENQRNEIEKKLTA
                     LLVQLPNLPHESVPEGKGAEDNIIERVGGKMPVLPSDALTHWELAKKYDLIDFELGVK
                     ITGAGFPVYKGKGARLQRALIDFFLDSARGAGYLEIQPPYLVNAASAFATGQLPDKED
                     QMYYCPQDDLYLIPTAEVPVTNLYRGMILNENELPIKNTAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQIQHPDKSYETLAEMVAYVESLVEKLELPWRTVRLCGGDMSFTSA
                     LTFDIEVFSAAQQRWLEVSSVSNFESYQANRLKCRFRTSDKKIQLCHTLNGSALALPR
                     IVAALLENNQTVDGI"
     gene            complement(8194..8457)
                     /gene="rpmA"
                     /locus_tag="PLR72_00875"
     CDS             complement(8194..8457)
                     /gene="rpmA"
                     /locus_tag="PLR72_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOK35798.1"
                     /translation="MAHKKGLGSSKNGRESESKRLGVKIFGGQLAKAGNIIVRQRGTV
                     HHPGENIGLGKDHTLYALVDGIVEFKKKKNNRSYVSIKPIEMN"
     gene            complement(8475..8789)
                     /gene="rplU"
                     /locus_tag="PLR72_00880"
     CDS             complement(8475..8789)
                     /gene="rplU"
                     /locus_tag="PLR72_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOK35799.1"
                     /translation="MYAIVEILGQQFKIEEGKKLFVHRMEEAERGSEIEFDKVLLIDK
                     NGDVTVGTPVIEGAKVKAEVLSHVRGNKIIVFHKKRRKGYKKHAGHRQNFTEILIKEI
                     VA"
     gene            complement(8919..9710)
                     /gene="cdaA"
                     /locus_tag="PLR72_00885"
     CDS             complement(8919..9710)
                     /gene="cdaA"
                     /locus_tag="PLR72_00885"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOK35800.1"
                     /translation="MGFQIGIKDIIDIFLVALLLYEIFKLLKRSGGINIFIGILTFII
                     CWFFVSYVFKMQLLGDIMTWFVNVGAFALIVIFQEEIRRFFSRIGSRREVGFLTKIKH
                     AFNNSKIQQEQTDYDIVQIVLACRNMSKSLTGSLIVITRRNDLDFYAQSGEQIDSKIN
                     SRLIESIFFKNGPLHDGALIISHRKLRAAACILPISKNQTIPKRLGLRHRAALGITEI
                     SDAIAIVVSEENGHISWAINGELTLNVKPEQLEHFLSTELAEDME"
     gene            complement(9738..10571)
                     /gene="folP"
                     /locus_tag="PLR72_00890"
     CDS             complement(9738..10571)
                     /gene="folP"
                     /locus_tag="PLR72_00890"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOK35801.1"
                     /translation="MHLKLNQQLFDLSSPLIMGIINITPDSFYKGSRFTTGKAILHAV
                     DEILENGGSIIDIGGFSTRPSAEIISEEEEIDRLSKALEIILKKYPQTVISIDTFRAK
                     VARYIVENFQVAMINDVSGGTLDDQMFETIADLKVAYVLMHMRGTPQTMQQLTDYEDV
                     VTEVFQFLQKRLAQLRSLGVTDVIIDPGFGFAKTIQQNYQLLKKLSYFKELGAPILAG
                     LSRKSMIYKLLNITPDEALTGTIAVNMLALIGGASILRVHDVKEAVQTIKIFNEYIAA
                     L"
     gene            11005..11865
                     /locus_tag="PLR72_00895"
                     /pseudo
     CDS             11005..11865
                     /locus_tag="PLR72_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
     assembly_gap    12291..12390
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12517..13914
                     /gene="asnS"
                     /locus_tag="PLR72_00900"
     CDS             12517..13914
                     /gene="asnS"
                     /locus_tag="PLR72_00900"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOK35802.1"
                     /translation="MKRTLVKEALKRNDFGAEVNIKGWVRTRRGNKNVSFIELNDGST
                     IHNLQIVVDNNKFGIDFLKPITTGACINVIGKLLPSMGKNQTIEIQTDKIEIYGTADP
                     ETYPLQKKEHTMEFLREIAHLRPRTNTFGAVFRIRHHMSIAIHTFFHERGFYYFHTPI
                     ITASDTEGAGQMFQVTTLNLNDIQKNEAGEVDYSYDFFGKQTGLTVSGQLEGELGALG
                     LGAIYTFGPTFRAENSNTPRHLAEFWMIEPEVAFNDINDNMILAQEFVQYCVRWALEN
                     CSDDLEFLNNRYDNDLIERLKFVVDNDFVHLTYTEGINILEEAIRNGHQFEFPVGWGL
                     DLQAEHERYLVENYFKKPVIVTDYPKDIKAFYMKQNDDGKTVRAMDVLFPKIGEIIGG
                     SQREDDYTKLKNRAKELKVPEKDIWWYLETRKFGNAPHSGFGLGFERFLLFVTGMSNI
                     RDVIPFPRTPKNADF"
     gene            13935..15794
                     /locus_tag="PLR72_00905"
     CDS             13935..15794
                     /locus_tag="PLR72_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOK35803.1"
                     /translation="MNGEMNNSKAIEIDNVVIRFSGDSGDGMQFTGTLFAKLAAILGN
                     EISTFPDFPAEVRAPQGTLEGVSGFQVHIGSKKIYTPGDEVDVLVAMNPASLKVNAKT
                     VKRNGIIIFDSDAFQTSNLQKAGFKTENPFEELSLPETIQLIPVPLTTLTKKSLEEFN
                     LDNKEVLQSKNMVALGLICWLFNRPIDKAYHFLEKKFNKKPNILHANIKALTDGFNYG
                     ENLQLSISTFIIEKSYETPKGKYTSINGNKAVAWGLIAAAEKAGKQLFLGSYPITPAS
                     DIMHELALRKDIGVKMVQAEDEIGGITMAIGASFAGCLAATSTSGPGMALKSEGMGLA
                     VMAELPLVIIDVMRSGPSTGMPTKTEQSDLFQALYGRSGESPLVVIAASTPDNCFNYA
                     YIASKIALEYMTPVILLTDGYLGNGSSLWKLSNLAELPEITPHYVKSEMEGCKVTIRD
                     EKTKIPYRSIPGMKGFCHCNGGLEKNYTTGNLSTDPINHEKMVLARQEKINNVANIIP
                     DIQIAGNKDADLLVVGWGSTYGHLISATNLLNNEGYKVALAHFNYLFPLPKNTEEVIK
                     RYKKVVVCELNTGQFAAYLSSKISQVNFFKYNRIQGQPFTVNELVAYFKQLLS"
     gene            15824..16843
                     /locus_tag="PLR72_00910"
     CDS             15824..16843
                     /locus_tag="PLR72_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOK35804.1"
                     /translation="METHNIQKYTVADFKSDQTPRWCPGCGDYGIYNSLLKTMAESGI
                     PPYETVIISGIGCSSRLPYYTKTYGFHTIHGRGAAIATGVKVANPQLTVWQITGDGDC
                     LAIGGNHFIHSIRRNVDINVLIFNNQIYGLTKGQYSPTTKKGFVTKSSPYGTIERPFR
                     LGELTLGARGTFFARTLDVDLVTSQMVMKAALHHKGTSVVECLVNCIIFNKGAHGWLS
                     EKETRADRTIVLEHGKRMIFGRNKDKGLILENNELKVVTIGENNITENDILIHDATTT
                     DYSLQMKLALMEGPDMPVALGIIRDVKEETFEDAMEKQIKSVQNSSTIKTFDDLIAQC
                     EQWEI"
     gene            complement(17075..17827)
                     /locus_tag="PLR72_00915"
     CDS             complement(17075..17827)
                     /locus_tag="PLR72_00915"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOK35805.1"
                     /translation="MKRLENKVAIVTGAARGMGKAEAELFAQEGAKVVLADILEKEVA
                     EVANQINKNGGEAIAFKLDVSKAKDWEKVVNEVVKKWGKVDVLVNNAGILSLSGIEET
                     SEEEWDKVMEINAKSDFLGIKYVLPAMKMAKKGSIVNISSIYGLIGSGAAVAYHASKG
                     AVRLLTKTVAAELAKYNIRVNSIHPGVIKTPMTEELLKDPANTKNLLGTTVLGRPAEA
                     IEVAYGALFLASDESSFITGSELVIDGGYTAL"
     gene            complement(18169..19992)
                     /locus_tag="PLR72_00920"
     CDS             complement(18169..19992)
                     /locus_tag="PLR72_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005807855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOK35806.1"
                     /translation="MISKLIQFIKIYLFFILIFLVQKPLFMLYYHNLYHDISFLDFVR
                     VLGNGLKLDASMAGYLTILPGIFLIMSLWISSKIINKFFKVYFAIVALLISIIFVADI
                     SLYGYWGFRLDATVLFYLKWPKEAMASVNMLTVIMGILVIAVIFILLYYLFTHFLLKE
                     KFSRKGNENKFLNAFVLLLFTGLLFIPIRGGFDVSTMNVSKAYFSSRIELNHAAINPM
                     FNFMESLFKEKDFDRQYRFMSDKEAHEEFKKLIDQPTDKEIPALFSLQRPNIILIILE
                     SFGSKIIEPLGGIPGIAPHLNHYCDEGILFTNFYANSFRTDRGLVSILSGYPAQPTTS
                     IMKYPRKSQTLPSIPRSLKKNGYKVAYYYGGDADFTNMRSYLISQGVDELITKQDFPR
                     KEHTMKWGVHDHIVFQRFLSDLQKEQKEPFMRIIQTLSSHEPFEVPYHKNNDSYLNSV
                     QYTDSCLGEFIEEFKKTKYWNNSIVLLVPDHAMHYPSSIDIRSVERYKIPFLILGGAV
                     ISHQKIDTYASQIDIAATLLHQLQISHQEFIFSKNILNPDSPHFAFYTFPNGFGFLTP
                     QNYYVYDCASQSVVINEGKKFENKKKGEAYLQILYDDLASR"
     gene            20285..21352
                     /gene="serC"
                     /locus_tag="PLR72_00925"
     CDS             20285..21352
                     /gene="serC"
                     /locus_tag="PLR72_00925"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOK35807.1"
                     /translation="MKKHNFNAGPSILPCEVIEKTAQAVLDFNGSGLSILEISHRSKD
                     FQAVVDEAVALMKELLNIPEGYSVIFLGGGASLQFCMVPYNLLEKKAAYLITGSWASK
                     AYKEAKGFGEAVEVASSKDANFTYIPKNYVIPADADYFHITTNNTIYGTELLKDIDSP
                     VPLVADMSSDIFSRPVDVSKYGIIYGGAQKNLGPAGVAFVIIKESILGKVSRYIPTML
                     NYKTHIENNSLFNTPPVLPIYSSLETLRWLKAQGGVKEMEKRSIAKSELLYNEIDRNK
                     LFVGTAAKEDRSRMNVCFVMKPEYQELEAEFLKFATDRGMVGIKGHRSVGGFRASLYN
                     ALPIESVQALIDCMQEFEKLH"
     gene            21482..22399
                     /locus_tag="PLR72_00930"
     CDS             21482..22399
                     /locus_tag="PLR72_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOK35808.1"
                     /translation="MKVLIATDKPFAKIAVDGIRKEIESAGFELALLEKYTEKQQLLD
                     AVKDVDALIVRSDIIDAEVIAAAKKLKIVVRAGAGYDNIDLDAATKAGICVMNTPGQN
                     SNAVAELVFGLLIYAVRNFFNGTSGTELRGKELGIHAYGNVGKNVARIAKGFEMKLYA
                     FDPYCQPEDIKKDGVQPLSSIESLYENCEIISIHTPATAETKKSINANLLQKLPKGAV
                     IVNTARKEVINEEDLIACMENRLDIKYVTDIMPDHHAEMVEKFPGRYFSTPKKMGAQT
                     AEANINAGIAAAKQIVDFFKNGNEQFRVN"
BASE COUNT         7803 a         3594 c         3607 g         7264 t
ORIGIN      
        1 aatcttattc attttatctt tatttttaat ttttcaggat tgaaaattaa aattcatatt
       61 cagtaaattt aaagaacaaa aaaaatcaaa attattttga atctacaata ccaatcatca
      121 aacaatcatt tattaaattc gcgtaattta atagctgtca gtattttttt agtatgtaat
      181 gaaaaatcac cattcataat ccaaccataa tatcctatat ctttttttag aacgtcggta
      241 actttttgtc ctttatattt tccaaaattg ataatctctt caccattttc attataaata
      301 attcgcccag caaaatcaac atttttagtt tgtgtagtaa aatcggagag gaaatccaca
      361 tcgtttttca attccgaata ccgatccagt tgagctttta aaatttcata agtagcctta
      421 gcatcagctt cagcgccatg tgcattcatt aaatctttgt cacaataaaa tttatatgct
      481 gctgataaag tgcgttgttc cattttatgg aaaataactt gaacatcaat aaatttatgt
      541 ttcattaagt cgatgtcgac atcagcacga ataaattcct cagccaaaag cgggatgtca
      601 aatttatttg aattataacc agctaaatca caaccttcta aatcacgtgc aatttctttt
      661 gcaacttctt tgaaagtggg acagtttttt acatcttcat ctgttattcc atgaatggcg
      721 gtagcttttt cagggatatg catttcagga ttaattcgaa tagttttagt ttcttctctg
      781 ccatttggat aaactttata gtaagatatt tcaacaattc tatctgtaac gatattggtc
      841 cctgttgttt ccagatctaa aaacgctaat ggttttttca attgaagctg cataataaag
      901 gatgggggtt taaattatat aataacttct tcttctaaaa ataataaaaa caaggtctaa
      961 agcaacctct ttagacctta ttataccata taagatttca tgattaatca tttaacaaca
     1021 taggcattag caacataacc acttcttcat tttctttctt ttcgaaagga aagaaaagag
     1081 caccatgagt tggatcagac aactcgataa ttacctccga tgagtcaagt gaggataaaa
     1141 tctctattaa aaaagttgct ttaaaaccta tagtaaatgt ttcaccttca aattgacagg
     1201 gaattacttc ttcagcagaa atagaaaaat caatatcttg agcagaaata tgaacttgtt
     1261 gattttcgaa aaccaattta atcaaattac ttgcctggtt tgaaaaaata gaaacacgtt
     1321 taatagcatt caaaaaattt accctatcaa taatgacttt ataaggattt gaagtaggaa
     1381 taacggcatt ataattggga tatcgtcctt ctatctgtaa accaatgagt gtataattac
     1441 ttaactggaa caaaacacgt ttttcgtcaa atttaattaa tacatctccg ctttctttag
     1501 gcaagatatt tttcaataaa ttagctggtt tttttggaag aatgaagctc attgcttcct
     1561 cttttaagga tgctgaagta taaattgttt tgaaacgcac caatttatgt gcatcagtag
     1621 ccactattgt caaaccttct ggaacgatat caaaataaat accgctcatc actggacgaa
     1681 aatcatcgtc tactgatgca aataaagttt tagaaattcc agaaaataaa ttgactcctg
     1741 gaacaactaa atttgaattt tcttcatcaa tttcaggaat acgaggatat tcatcagcat
     1801 tttgtccaat aaaattataa gttccattgg ctgacaaaat ggtaagtgat aaatttgtat
     1861 cattaataga aaaagtaaga ggctgttctg aaaattcacg taatgtgtct aataataacc
     1921 gggaagttat agcaactctt ccacttccct tggcattaat tacttccaaa gaggtaatta
     1981 aagtggtttc aatatcagtt gcaacaaccg ttaaagtatt tcctttgagc tcaaataaaa
     2041 aattatccaa aatctgcaaa gcatttttgg gattaattac tcgactgatt gtctgtaaac
     2101 gattcaaaag ttcggtgcta gaaacaacaa attccatatc attcctcttg gttaatttat
     2161 gaatattttt aattgaacaa atgtacagat ttttttaaaa aataaaaaat tatatcttct
     2221 tttttccaaa tggttgaata ttgaatattt ttcataaaag taatgatttt tgaagtctct
     2281 cagcaaggaa aggaaaatcc gacaaaagtt gctctttacg atgctgaata tgaagtaaaa
     2341 attctttata ttcttccgat tcagggtgca taggtttatg tttgaaatcg tttaataatt
     2401 gctcaacacg ttgaacaatt ttttgtgtaa tttctgaaaa ataagtatct gaaaattttt
     2461 tccaaatgta tttaatagcg aaagcagaag gatgaatcat atcctcatca taaaacctat
     2521 aatcccgaag ttcgtccatc aaaatttcat aagcagaaaa ataagtcaca ttttcaaatt
     2581 ttttctgtaa tgcatctatc gccaaaaata aaatactttt actcacattg ttttcatacg
     2641 ccccatcctt ccaatatcga atagggctga tactaaaaat aatataaagt tgcggaaata
     2701 aagactgaag tttttttatt aaatcatcgt aattagcaac aatctcttct accgtcaatc
     2761 ttctacgcaa aaatgctgag gatggtagtt tgtgacaatt agcaactatt ctgccacttt
     2821 ttctttcttc ataaatccac gccgttccaa aagtaattaa aagaaaattg gcatgacgta
     2881 aaaattctga agctgcatca agtcttgttt gaattttcat taaacatttc tcagaagaaa
     2941 tatctgaaaa gtaactgctg tgagaaaaac tatgccagag tgagcgatat tgaaaaatat
     3001 cattttgagt aaattgtttc ttttcaagaa gcatttcaag gcttttgctt atcgagaaag
     3061 gattataaag tactccaaaa ggattcattt caactgaaaa acagttttct attagttttt
     3121 gtccaatatt ttcagaaaaa caggatccaa gagttataat attgtcctga taagtaattt
     3181 ccttttgaga atgagaaata tctattgaag taataaaaaa attattattc atcttttctt
     3241 taaaatcctt tggtttcaca aaataataat tttaaaaatt atgctaaaaa gatcaaaaat
     3301 taccatcacc taaatttata tttatccctt aaatttttat taccgttatc aatatttttt
     3361 tgtaaatgta taaaagttct aagaatattt attatatttg tatagaaaat cgagaaaaaa
     3421 tatttcgaaa gagttctttt acatacattt tttacaccaa aacagataat aatcaaaaag
     3481 caggtatact caagtaaaaa atatttttca agaaagttcg ttttaagcac gatttttgta
     3541 gtgcatttta agttcagttt tttattttta ctctaaaaac ttattttatg aaaaacgttc
     3601 aatttgaaaa agagttgtta gcactgcaaa gcaacatgcg taatttcgct ttttcattga
     3661 ccatgaatcg cgacgacgct gaagatttat tgcaagatac aacattgaaa gtgttaaaca
     3721 acaaagataa atttactgaa aatatcaatt tcaagggatg ggtactaacg ataatgaaaa
     3781 atatcttcat caataactat cgaaaaattg ttcgcaatca aacagttatt gataaaacag
     3841 aagatttaca tcatttaaat ttaccacaaa attctggttt tgagagccca gaagggtctt
     3901 attcgattcg tgaaatcaac aacaatattg atgcttttcc tgatgaatat agaattcctt
     3961 tttccatgca cttgcaagga tacaaatatg aggaaatagc tcaaaccatg aacctaccgc
     4021 tgggaactgt taaaagtaga atttttttag cacgtaaacg tttacaagaa cagttgaaag
     4081 attatcgtga ttagtaaagt ataagtttaa aaaaaccgaa gaacaaaccc ttcggttttt
     4141 ttgtttttta ataatatatc taaatccttt ctttgtaatt tttaattagt ttactcatga
     4201 atacggcaat tgtaatgctt ggaagcaatt ttaatgcaga tgagaacatt gatcttgcta
     4261 aagaaaaact tgctgaatat tttgaaatcg cttctttcag ctcacgaata gtcacagatc
     4321 cagagggtga ttatccttat aaattttata atgaagccgt taaacttttt tctgatgaaa
     4381 ctgctaaaga aaccatcaat attctcaaac aaattgaaaa agagttaggt cgtaccccag
     4441 aaagtaaaca aacaaaatta attcctatcg atattgatct tattttttgg aatgaaactt
     4501 taataaaaga tgattattat cgttattcat ttgtaaaaaa atgtattgat gaaatcaaat
     4561 aattaatcgg attcaaaaaa tgtttttcat tcatatattt aatcaaaaat gatttatcaa
     4621 aaagaaatta cgctggttcc tcattcaaaa ggttttcatt taattacttc tgaaatcatc
     4681 aacaatttac ctccacttcc tcaggaagga ttattgcatt tattccttaa acatacttct
     4741 gctgctttaa ctatcaatga aaatgccgat ccagatgtac aaagtgattt gaaaaaaatc
     4801 tttgatcatt tagtaccggc gcatcaaccc tattatgatc atactttaga aggagacgat
     4861 gatatgccag ctcatgcaaa atccacatta gctggagtaa gtttattaat ccccatttcg
     4921 aatagcagat taaatttagg tatttggcaa ggcatttatc tctgtgaatt cagacaatat
     4981 ggcgggaaac gcaaaatagt tgctacgata attagttaat acactgtata acagtttgct
     5041 aactttataa atcccgctaa atgatttctt ttgatttttt taaaaacagc ttttttcaga
     5101 gagtagaatc aagccattta aaaatattgc taactttgca cttcgaaaaa caaatttttt
     5161 attgaataat aattttattt caattagcag ataacaacaa attatggcaa attctttaat
     5221 atctcgttcc gaaaattatt ctcagtggta taatgattta gtagtaaaag ctgatctggc
     5281 agaaaattca gcagtgcggg gttgcatggt gataaaaccc tatggatacg ctatctggga
     5341 aaaaattcaa tcagagcttg atcgcagatt taaagaaacg ggacatgtaa atgcttattt
     5401 tccacttttt attcctaaat cttttttaag taaagaagca gaacactttg aaggatttgc
     5461 aaaagaatgt gctgttgtta cccatcatcg gttgaaaacc gatccaaacg gaaatggttt
     5521 aattgtcgat cctgatgcca aattggaaga agaattgatt gtacgaccta cttctgaaac
     5581 gattatctgg aacacatata aaaattggat acattcttat cgagatcttc ctcttcttat
     5641 taatcagtgg gcgaatgtag taagatggga aatgcgtacc cgcctttttt tgcgaacttc
     5701 agaatttctc tggcaggaag gtcatacagc ccatgcaact gaaaaagaag ctatagaaga
     5761 agcccaaaaa atacttcaac tttatactga ttttaccgaa caatttatgt ctattcctgt
     5821 aattaaagga gtgaaatcag ccaatgaacg atttgcgggt gcagtagaaa cattctgtat
     5881 tgaagctatg atgcaagatg ggaaagcttt acaaatgggg acttcccatt ttttaggaca
     5941 gaattttgct aaggcttttg atgtaacttt tgtagataac aataatcaat tgcaatacgt
     6001 gtgggcaact tcgtggggag tttcaaccag actaatggga gccttgatta tgacacattc
     6061 cgatgacaat gggctggtgc ttccgccgaa tttagctcca tttcaagtaa ttattgttcc
     6121 catttataaa aatgagaaac aattagctga catttccttt gttgctgaca aaataatggc
     6181 agacttaaaa aaagcgggta tcagtgtgaa atatgataat gacgatacaa aaaaacctgg
     6241 ttggaaattc gctgagtatg agctgaaggg tgttcctatt cgtttggcaa tgggacccag
     6301 agatattgaa aatcacacag ttgaaattgc tcgacgtgat acattaacaa aagaaactat
     6361 ttcaatggaa aacgtggtta atgaaatttc acaacaactc aagaatattc agaaaaatct
     6421 ttacgagcgc gcattaactt ttcgaaatgc aaatatgaag gaaataaatt cgtataagga
     6481 attcaaggaa aaaattgaaa aaggaggttt tttgctgtgc cactgggatg gaacttcgga
     6541 aacagaagaa aaaataaaag aagaaacgaa agccaccatt cgttgcattc ctttcgattt
     6601 agataaaaca ccgggcaaat gtatggtaac aggcaaacct tcggctcaaa gagttgtttt
     6661 tgcaagagct tactaatgtt tgtaacatta aattattann nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnntt tataccatca accgtttgat tgttttccag caatgcagcc
     6841 acaattcgag gtaaagccaa agcactacca ttcaaagtat gacaaagttg aatcttttta
     6901 tcagaagttc taaatctaca cttcaaacga tttgcttggt aactttcaaa attagaaact
     6961 gaacttactt ccagccagcg ttgttgagca gccgaaaaaa cttcaatatc gaaagttaga
     7021 gccgaagtaa aactcatatc tcctccacaa agacgaactg ttcgccacgg taactccaat
     7081 ttttccacca gcgattcaac ataagcaacc atttcagcaa gcgtttcata agatttatca
     7141 ggatgttgaa tctgaacaat ttctacttta tcgaactgat gaagacgatt taagcctcga
     7201 acatcttttc cataagaacc agcttcccgg cgaaaacaag cagaatatgc agtattcttg
     7261 atgggcaatt cattttcatt taaaatcatg ccacgataaa gatttgtcac aggaacttcg
     7321 gcggtaggaa tcaaatacaa atcatcctgt gggcaataat acatttgatc ttctttatca
     7381 ggcaattgtc cggtagcaaa agccgaagca gcattgacca gataaggagg ctgaatttcc
     7441 agataaccag ctccacgagc agaatccagg aaaaaatcta tcaaagctcg ttgtaatctt
     7501 gcacctttcc ctttataaac aggaaaacct gctcctgtaa tttttactcc taattcaaaa
     7561 tcgattaaat catatttttt tgccaattcc caatgggtta aggcatccga aggcaatact
     7621 ggcatttttc caccaactcg ctcaatgata ttatcttctg ctccttttcc ttcaggaaca
     7681 ctttcatgag gcaaatttgg aagttgaact aaaagagcag ttaatttttt ttcaatttca
     7741 ttccgctgat tttccagaga tttgatggat tcttttaact taattgttct atctttaaga
     7801 gcattaactt cctcttgttt accttgtttt ataaaaacac caatttcttt tgatagttta
     7861 tttatttcag cagccgcctg atcactttca aactgtgttt tttttcgctg attatcaagt
     7921 tcaattactt gagaaacaat ttcacgagca tcaaaatgtt tcttttccaa ccgtctaata
     7981 acatcttcgg tattttcaat gatatatttt aaagtgagca tattgtcaat aaattatttt
     8041 aatggattta tgtttacatt caacaagata gatattttat gcaaaaataa aaataatttt
     8101 aaaagttaat aaagaaaaaa tctcccatca ttttactgac agtaaaataa taggagattt
     8161 ttaagttttt gtacttttgc ttaaaaaatg aaattaattc atttctatag gcttaataga
     8221 aacataagaa cgattgtttt ttttcttttt aaattctacg attccgtcaa ccaaagcata
     8281 taaagtatgg tctttgccta aacctatatt ttctcccgga tgatgaacag ttccacgttg
     8341 gcgaacaata atatttccag ctttggcaag ctgtccgcca aatattttaa ctcctaatcg
     8401 tttactttcc gattcacgac cgttttttga actccctaaa ccttttttgt gtgccatttt
     8461 tttatctatt tgaattaagc aacaatttct ttaattaata tttccgtaaa attttgacga
     8521 tgtcctgcat gttttttgta acctttccga cgctttttat gaaaaacaat tattttatta
     8581 ccccgaacat gcgacaagac ttctgccttc acttttgctc cctctattac aggagttcca
     8641 acagtaacat cgccattttt atcgataagc aatactttgt caaattcaat ttcagaacct
     8701 ctttctgctt cttccatccg atgaacaaac aattttttcc cttcttcaat tttaaactgc
     8761 tgacctaaaa tttctacaat tgcatacatt ttcaattctt tttaaattct atcggtaagg
     8821 tgagataaaa tcttcttaaa aaaccttatc caaaacgacc tgcaaattta caaatttatt
     8881 tttgttttac aaaaattatc ctaaaaattt tataaaattt attccatatc ctcagccaac
     8941 tcagttgata aaaaatgttc taattgttca ggctttacat tcaatgttaa ttcaccattt
     9001 atagcccatg aaatatgtcc attttcttcg gaaaccacaa tagctattgc atccgagatt
     9061 tctgttatcc ctaatgcagc tctatgccgt agtcctaatc gttttggaat ggtttgattt
     9121 ttagaaatag gtaaaataca ggcagcagct ctcagttttc tatgactaat aatcaatgcc
     9181 ccatcatgga gaggaccatt tttaaaaaaa atactttcaa taagtcttga attaattttt
     9241 gaatcaattt gctcacccga ttgagcataa aaatccaaat cattgcgacg ggttatcaca
     9301 attaagcttc ctgttaagct tttagacata tttctgcatg ctaatacaat ttgaacaata
     9361 tcataatcag tttgttcctg ttgtatcttt gaattattaa aagcatgctt aatcttggta
     9421 agaaagccaa cttcccgtcg tgaacctatt cttgaaaaaa agcgacgtat ttcttcctga
     9481 aaaattacaa ttaacgcaaa agcacctaca ttgacaaacc aggtcataat atcgcccagc
     9541 aattgcattt tgaaaacata agacacaaaa aaccaacaaa taataaatgt caaaatacct
     9601 ataaaaatat ttattcctcc tgagcgtttg agaagcttaa aaatctcata gagcaaaaga
     9661 gcaactaaaa aaatatcaat aatatcctta attccaatct gaaatcccat aaattattta
     9721 agtattttat tatcagttta aagagcagct atatattcat taaaaatttt tattgtttgc
     9781 accgcttctt ttacatcatg tactcgcaaa atagaagctc cgccaatcag agccagcatg
     9841 tttacagcaa tagttcctgt caaagcttcg tcaggggtaa tgttcaatag tttataaatc
     9901 attgattttc tggataaacc agctaaaata ggagcaccga gttctttaaa ataagacaat
     9961 ttttttaata actgataatt ttgttgaatc gtcttggcaa aaccaaaacc aggatctatt
    10021 atcacatctg ttacgcctaa tgacctcaat tgagccagac gcttttgtaa aaactgaaaa
    10081 acttcagtaa caacatcctc ataatctgtc agttgctgca tagtttgtgg agtaccacgc
    10141 atatgcatta acacatatgc aacttttaaa tcggcaatag tttcaaacat ttggtcatcc
    10201 aaagttcctc cacttacatc attaatcatt gcaacttgaa aattttcaac tatatatctg
    10261 gcaacttttg ctctaaaagt atctatagat ataacagttt gaggatattt ttttaaaata
    10321 atttccaaag ctttggataa acgatctatt tcttcttcct cagatattat ttcagcagaa
    10381 ggtcttgtag aaaatcctcc tatatcaata atactgccac cattttctaa aatttcatct
    10441 acagcatgca aaatagcttt tcccgtagta aagcgacttc ctttataaaa agaatcagga
    10501 gtaatattaa taatccccat tataaggggc gaagaaagat caaaaagctg ctgattcaac
    10561 tttaagtgca taaaaaaata atttcaataa attcttgatc aaaataatta tcagaagtgc
    10621 aaaaataaaa aatttaaatg attgaaaata atggaaaata aaaaaatcaa aatattaact
    10681 atccatatat acgtttttca gaaaaaatca gttattattt tgtaaaataa aatcaattta
    10741 tttgataaaa aaacaaaaat aaattaataa aatactatat ttgcaaggaa tttttaaaag
    10801 tgagattata aataaagttc ttttattatg aatgtaaaaa ccttaataca attttctgct
    10861 ttggcaacta tcttattatt agcaagttgt gcaagtcaat ctaaaaaaga aaattattca
    10921 cgagttacag ggtggaaata taatgatgaa aaaggaacag gatttggagt aaaaaaagat
    10981 tttaagacga cagttccccc tggtatggtt tccattgaag gaggttcgtt tactattgga
    11041 gaaaaaggag aagtggtttt agctccaaga aatagtagac gacgcagaat aacggtaagt
    11101 tctttttaca tggaccagta tgaaattcgt aatgtagatt ggcgtgaata tactcactgg
    11161 atggaaacgg tttttggaaa aacagctccc aagcttgttc agaaaacata tcctgatggt
    11221 aaagtttggc gagaagaatt agcttacaat gaaccttaca tagaacatta ttttactcat
    11281 ccagcttttg atcaatatcc cgtagtagga gtgacgtggg aacaagcaat ggattactgt
    11341 agctggcgta cagaccgtat taatgaattg gctttaatta atgcaggagt aattgttccc
    11401 cccgattttg cttccttaaa aaatctatca tttgattcca ttattcataa ttttattttt
    11461 aatacccaaa aatatttaaa acaacctact tatcaaccaa aggctggtcc aaaacctaaa
    11521 aaagatttat atggacaaat ccggaaagca gacatgtctg atggtatttt acttgtctcg
    11581 gatataagac tcccgaccga agccgaatgg gaatatgcag cgtatgcatt aaaagcagac
    11641 aaaaatggct ttgttcctgc agggaaaatt tatccctgga atggttatgg agttagaaat
    11701 ccatcaaaaa aagagttagg gcaaatgatg gcaaattttg tccgtggacg tggcgatatg
    11761 atgggaacag caggcgcttt aaatgacaga gctactatta cagcaccggt agattcttat
    11821 tatcctaatg atttcggact ttacaacatg gctggaaatg tgaatgaatg ggtgttggat
    11881 gtataccgcg tcacaagttt tgatgatgtg agtgaataca actcgtttcg gggtaatgtt
    11941 tatcctactt acatagtaga aagagaagat gaattgggaa acaatattta taaaattgac
    12001 tcgctcggaa gaatagcaac aacggttttg aaaaaagatg atgtaagaaa ttataaagat
    12061 ggtgatcctg cttcaaaact ttctactgat tttgtaatgt atggggctga ttcaactgat
    12121 attgccgcca ttaccaatat gacaaaaatt gatccaaccg atgtcctggc accacaaata
    12181 acagaaaaaa caagagtata taaaggtggc tcatggaaag atcgtgttta ttggttaaat
    12241 ccttctacca gacgttatct ggatcaagac caatgtgcta atgatattgg nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcttttttct ctgaattatt ttttattagc
    12421 acctgtacag caaagttcag gtgctgtttt gtaaatatcc ctcatatatc aattgaaatt
    12481 cgtaattttg caaattgaat aaataaagaa tttaagatga aaaggacact tgtcaaagaa
    12541 gcattaaaaa gaaatgattt tggagctgaa gtaaatatca aaggatgggt acgcactcga
    12601 cgagggaata aaaatgtgag ttttatagaa ttaaatgacg gttcaactat tcacaatcta
    12661 caaattgtgg tggataataa taagtttggt attgactttt taaagccaat tactactgga
    12721 gcttgcatta atgtaattgg aaaactttta ccttcaatgg gtaaaaatca aacaatagaa
    12781 attcaaacag ataaaattga aatttatgga actgctgatc cagaaactta tcctcttcag
    12841 aagaaagaac atactatgga atttttacgt gaaattgctc acttgcgtcc ccgtactaat
    12901 acttttggag cagtttttcg cattcgtcat cacatgtcaa tagccattca tacatttttt
    12961 cacgaaagag gtttttatta ttttcatact cccattatca ctgcttccga tacagaaggt
    13021 gcgggacaaa tgtttcaagt taccacttta aacttgaatg acatacaaaa aaatgaagca
    13081 ggagaagtgg attattctta tgacttcttt ggcaagcaaa caggattaac tgtctctggt
    13141 caacttgaag gagaattagg agcattgggc ttgggtgcta tttatacttt tggacctact
    13201 tttagagccg aaaattctaa tacacctcga catttggctg aattctggat gatcgagcca
    13261 gaagttgctt tcaatgatat taatgataat atgatattgg ctcaagaatt tgttcaatat
    13321 tgtgtccgtt gggcattaga aaattgttcg gatgatctgg aatttttaaa taaccgttat
    13381 gataatgatt taattgaacg attgaaattt gtagttgaca atgattttgt tcatttaact
    13441 tatactgaag ggattaatat tttggaagaa gctattagaa atgggcatca atttgaattt
    13501 ccagtaggtt ggggattgga tttacaggca gaacatgaaa gatacttggt agaaaattat
    13561 tttaaaaagc cagttattgt aactgattat cccaaagata taaaagcttt ttatatgaaa
    13621 caaaatgatg atggcaaaac tgttcgtgct atggatgttc tttttcccaa aataggcgaa
    13681 attattggag gttcacaacg ggaagacgat tatacaaagc tcaaaaatcg ggctaaagaa
    13741 ttgaaggtac ctgaaaaaga tatatggtgg tatttggaaa ctcgtaaatt tggaaatgca
    13801 cctcattcag ggtttggact tggatttgaa cgtttcttat tatttgttac aggaatgtca
    13861 aatattcgag acgttattcc tttccctcgt acacctaaaa atgcagattt ttaaacagtt
    13921 gaatatttta taagatgaat ggagaaatga ataatagtaa agcaattgaa attgataatg
    13981 tcgtcatacg tttttctgga gattcaggtg atggaatgca atttacagga acattgtttg
    14041 caaaactcgc ggctatttta ggaaacgaaa tatcaacttt tcctgatttc cctgctgaag
    14101 tacgtgctcc gcaaggaaca ttggaaggtg tttctggttt tcaagttcat ataggttcaa
    14161 aaaaaattta tacacccggt gatgaagtag atgtattagt agcaatgaat ccagcctcct
    14221 taaaagttaa tgccaaaaca gttaaaagaa atggaattat tatttttgat tctgatgcat
    14281 ttcaaacttc caatttacaa aaggcaggtt ttaaaactga aaatcctttt gaagaattat
    14341 cacttcctga gacaattcaa ttaattcctg tccctcttac tactttaact aagaaaagtt
    14401 tagaagaatt caatctggat aataaagaag ttcttcaaag taaaaatatg gttgccttgg
    14461 gattaatttg ctggttattc aatcgtccca tagataaagc ttatcatttt ttagaaaaaa
    14521 aatttaacaa aaagcctaat atcttacatg ctaatattaa agctcttacc gatggtttta
    14581 attatggaga aaatttacaa ttatccattt caacttttat tattgaaaaa tcatacgaaa
    14641 caccaaaagg aaaatatacc agtattaatg gaaacaaagc tgtggcgtgg ggattaattg
    14701 ctgctgctga aaaagcaggt aaacagttgt ttttaggaag ttaccctatt actcctgctt
    14761 cagatatcat gcacgaatta gccctgagaa aagatattgg agtaaagatg gttcaggcag
    14821 aagatgaaat tggaggtatt actatggcta ttggtgcctc tttcgcaggt tgtctagcag
    14881 caacaagcac ttccggacca ggaatggctt taaaatcaga aggaatggga ttggccgtaa
    14941 tggcagaatt gccccttgtg attattgatg ttatgcgaag cggaccttct acaggaatgc
    15001 ctaccaaaac agaacaaagc gatctttttc aagctcttta tggaagaagt ggcgaaagtc
    15061 ctttggtcgt aattgcagca agtacacccg ataattgttt caattatgct tatattgcaa
    15121 gcaaaattgc attggaatat atgactcctg taattctact gactgatggg tatttgggga
    15181 acggctcctc tttgtggaaa ttatccaacc ttgcagaatt accagaaata actcctcatt
    15241 atgtaaaatc agaaatggaa ggatgtaagg taactatacg tgatgaaaaa acaaaaattc
    15301 catatcggag tattcctgga atgaaaggat tttgccattg taacggtgga ttagaaaaaa
    15361 attacactac tggaaattta tctaccgatc ctattaatca tgaaaaaatg gtattagcca
    15421 gacaagaaaa aataaataat gtggcaaata taattcctga tattcaaatt gcaggaaaca
    15481 aagatgctga tttattggtc gttggttggg gaagcactta tggacattta atcagtgcta
    15541 ctaatttatt aaataatgaa ggttataaag tagcattggc acattttaac tatttgtttc
    15601 ccttaccaaa aaatacggaa gaagttatca aacgctataa aaaagtagtc gtttgcgaat
    15661 taaatacggg acaatttgcg gcttatttat cttcaaaaat tagtcaagtt aactttttca
    15721 aatacaatag aattcaagga caacctttta ctgtaaatga attggttgca tatttcaagc
    15781 aattactttc ttaatttttt caaaaaaata gaaacttttg actatggaaa cacataatat
    15841 tcaaaaatat actgttgcag atttcaaaag tgatcaaaca cctcgttggt gccctggttg
    15901 cggtgattat ggtatttaca attccttatt aaaaacaatg gctgaatcag gaatccctcc
    15961 ttacgaaact gtaattattt caggaattgg ttgctcttca cgtttgccct attacacaaa
    16021 aacttatgga tttcatacca ttcatggaag aggggctgct atagcgacag gtgttaaagt
    16081 tgctaatcct caacttactg tttggcaaat tacaggcgat ggggattgtc ttgcaatagg
    16141 tggaaaccat tttattcata gtataaggag aaatgtagat atcaacgtac ttatttttaa
    16201 taatcaaatc tatggattaa caaaaggtca atattctcct acaacaaaaa aaggatttgt
    16261 taccaaatca tctccttatg gaaccattga acgtccattt agactaggag aactcacact
    16321 tggagcaaga ggcactttct ttgctcgcac attggacgta gatttagtta cttctcaaat
    16381 ggtgatgaaa gcagctttac atcacaaggg gacgtcagtt gtagaatgtc tggtcaactg
    16441 tatcatattt aacaagggag cgcatggctg gttgtcagaa aaagaaacca gagccgatag
    16501 aacaattgtt cttgaacatg gaaaacgcat gattttcgga agaaataaag ataaaggtct
    16561 gattttagaa aacaacgagt tgaaagtagt aaccatcgga gaaaataata tcactgaaaa
    16621 tgatatttta attcatgatg caacaactac tgattatagc ttgcaaatga aacttgcatt
    16681 aatggaaggt cctgacatgc cagtagcttt aggcattatc cgtgatgtga aagaagaaac
    16741 ttttgaagat gcaatggaaa aacaaattaa aagtgtacaa aactcttcta ctattaaaac
    16801 atttgacgac ttgattgctc aatgtgaaca atgggaaata taatcaatcc tcgcttgctt
    16861 ttaaattttc atgataattt tcgaaaaatc aaataattaa cgcaactttt aagggaaaat
    16921 aatgaggcaa agaaatattt ttatcttgaa ggaataaata ctttactttt ttgtcttttt
    16981 ataccagtga caaagtgata aatatatttt taagtattat tgttttctac acgagaggcg
    17041 tcaattttat taacacctct cgtatagtat atagttaaag agcggtgtat ccaccgtcta
    17101 ttactaattc agaaccagtt atgaaggaag attcatcgga agccaaaaat aatgctccat
    17161 aagctacctc aatagcttca gcaggtcttc ctaaaacagt tgttcccagc aaattctttg
    17221 tattggctgg atcttttaat aattcctcag tcataggagt tttaattacg cctggatgaa
    17281 tagagttaac acgaatatta tatttggcta attcagcagc tacggttttt gtcaacaatc
    17341 gaacagcccc tttagaagca tggtatgcca cagccgcacc actgcctatt aaaccataaa
    17401 tagaagagat attaactata gagcctttct ttgccatttt catcgctggc aatacatatt
    17461 taatgcctaa gaaatcactt ttggcattaa tttccataac tttatcccat tcctcttcgc
    17521 tagtttcttc tatgcctgac agactcaaaa taccagcatt attaaccagc acatctactt
    17581 tgccccattt ttttacaact tcattcacca ctttttccca atctttagct tttgaaacat
    17641 ccaatttaaa agcaatagct tcaccaccgt ttttgttaat ctggtttgcc acttctgcta
    17701 cctccttttc taaaatatca gccaatacca ctttagcacc ttcttgggca aataattcag
    17761 cttcagcttt acccatacct cttgctgcac ctgtcacaat tgcaactttg ttttctaatc
    17821 ttttcataat tttttatttt attagttata cattaaattt gacaagatca tttataaaaa
    17881 actttatttt taataaaagt caaatttaaa attttaattt tgaatttaca aagttttgct
    17941 taaaaaactt gcgtgatgtt atttttaaca gctttaaaaa ttttttttat gcttccctat
    18001 aaagtaaaaa caaaatttat ttatttattt tgttgaattt ccaattgaaa aaggtattta
    18061 tgaaggggaa caaaaaagtt tagaaagagg tttttttagg ttgttttttc gtaataattc
    18121 agaagtattt ctaatctttg tctaaaattt ttttgaaaaa tatacttttt atctacttgc
    18181 cagatcatca taaagaatct gaagataggc ttcacccttt tttttgtttt caaatttctt
    18241 tccttcattt attactactg attgggaagc acaatcgtac acataataat tttgaggagt
    18301 taaaaaacca aaaccattag gaaaagtata aaaagcaaaa tgaggagaat caggattcaa
    18361 aatattttta ctaaaaataa attcctgatg agaaatttgc aactgatgca ggagggtagc
    18421 agcaatatct atttgtgaag catatgtatc aattttttga tgggatatta cagcacctcc
    18481 taaaataaga aaaggtattt tgtaacgttc aacagaacgg atatcgatag aggatggata
    18541 atgcatggca tgatcgggaa ccagtagtac aattgaatta ttccaatatt ttgttttttt
    18601 aaattcctca atgaactcac ccaagcagct atccgtatat tgaacggaat tcaggtaaga
    18661 atcattgttt ttatggtaag gaacttcaaa aggttcatgg ctgcttaatg tctgtataat
    18721 tcgcataaaa ggttcttttt gttccttttg taaatcggac agaaaacgtt gaaacacgat
    18781 atgatcatgg acgccccatt tcattgtatg ttcttttctg ggaaagtctt gtttcgtaat
    18841 aagttcatcg actccctgtg agattaaata agaacgcatg tttgtgaaat cagcatctcc
    18901 gccataataa tatgctactt tataaccatt cttctttaat gaccggggga tagaaggtaa
    18961 cgtttggctt tttcgtggat atttcataat agaagtggtg ggttgggcag gataaccact
    19021 taaaattgaa acaagtccac gatcagttct gaaactgttt gcataaaaat tagtaaataa
    19081 aattccttca tcgcaatagt gattcaaatg tggagcaatt cctggaattc cacccagagg
    19141 ctcaattatt ttcgacccaa aactctccaa tataatcaga ataatattgg gacgttgcaa
    19201 ggaaaataaa gcaggaattt ctttatcggt aggttgatca atcaattttt taaactcctc
    19261 gtgagcttct ttatcgctca taaaacgata ttggcggtca aagtccttct ccttaaagag
    19321 agattccata aaattaaaca taggattaat agcggcatga ttcaattcga ttcgtgaact
    19381 aaaataagct ttactgacat tcattgtgga aacatcaaaa ccgccgcgaa taggaataaa
    19441 aagtaaacca gtaaataata acaatacaaa agcattcaga aatttatttt catttccctt
    19501 tcgagaaaat ttctctttca ataaaaaatg agtaaacaaa taatacagta aaataaaaat
    19561 gacggctatt actaatatcc ccataataac agttagcatg tttacacttg ccatcgcttc
    19621 ttttggccat ttcaggtaaa aaagcaccgt cgcatccaat cgaaatcccc aataaccata
    19681 cagggaaata tctgccacaa aaattattga aataagcaaa gctacaatag caaaataaac
    19741 tttaaaaaat ttattgataa ttttagatga tatccacaat gacataatta gaaaaattcc
    19801 cggcaatatc gtcaaatagc cagccataga tgcatctaat tttaatccgt tgccgagcac
    19861 acgaacaaaa tcaaggaaag agatatcgtg atacaaatta tggtaataca acatgaaaag
    19921 cggtttctga acgaggaaaa tcaggataaa aaagagataa attttaataa attgaataag
    19981 tttgctgatc atttatgaga aaaaattaat atctgactat aaaaattgca gttgagataa
    20041 ttttttacaa aattgcaaat ttcttgtcta ttaacaatat taaaaaatta cacaaagata
    20101 gtttttttgt gcaattttct ttctcaaata aaatattttt tctcaaaaaa acgtacaaat
    20161 tgaaaagttt aaagataaat attaaaaaat tagtcattaa aaagaataaa tttaaaaaaa
    20221 aatgtaactt tgctttttca tttgaaaagt aacaaatttt acaaataatc atttaatttt
    20281 tgatatgaaa aagcataatt ttaatgcagg gccttctatt ttaccttgcg aagtaattga
    20341 aaaaacagct caagctgtgt tggattttaa tggttcaggt ttgtctattc tggaaatcag
    20401 tcatcgatca aaagattttc aagcagttgt agatgaagca gtagcgttaa tgaaagaatt
    20461 gctcaatatt cctgaaggtt attctgtgat tttcttagga ggtggagcaa gtttacaatt
    20521 ttgcatggtt ccatacaatt tgctggaaaa aaaggctgct tatttaatta ctggcagttg
    20581 ggcaagtaaa gcttacaaag aagcaaaagg ttttggagaa gcagtagaag tagcatcttc
    20641 aaaagatgcc aactttactt atattcccaa aaattatgtg attcctgccg atgcagatta
    20701 ttttcatatt actaccaata acactattta tggaactgaa ttgttaaaag atattgactc
    20761 gccagttcct ttagtagctg atatgtcttc tgatattttt tctcgtcccg tggatgtttc
    20821 aaaatatgga attatttacg gtggagctca aaaaaattta ggaccagcag gtgtagcatt
    20881 tgttattata aaagaatcta ttttaggaaa agtatcgcgt tatattccta cgatgcttaa
    20941 ttataaaacc catattgaaa acaattcttt gttcaataca ccacctgttc ttcctatcta
    21001 ttcttctctg gaaactttac gctggttgaa ggcacaagga ggagtaaaag aaatggaaaa
    21061 acggagtatt gcaaaatcag aattacttta caatgaaatt gaccgaaaca aactttttgt
    21121 tggtacagca gctaaggaag atcgttctcg aatgaatgta tgttttgtta tgaaacctga
    21181 atatcaggaa cttgaagcag aatttttaaa atttgctacc gatcgcggca tggtaggcat
    21241 caaaggtcat cgctcagtag gtggtttcag agcttctctt tacaatgctt tgcctattga
    21301 aagtgttcag gctttaattg attgtatgca ggaatttgaa aaattgcatt aattttttaa
    21361 tttactttga tagatttcgt tataaataag aaaatggtaa tgatttctct tttataagga
    21421 gaaaacattg aaaaaaagtt ttgcttctat atgataagtt aaacctttaa aattatttta
    21481 tatgaaagta cttattgcta ccgataaacc atttgcaaaa attgcagtgg atggcatacg
    21541 aaaagaaatt gaatcagctg gttttgagct ggcattgctt gaaaaatata cggaaaagca
    21601 acaacttctg gatgcagtga aagatgttga tgctctgatt gttagaagtg atattattga
    21661 tgctgaagtt attgctgctg caaaaaaatt aaaaatcgtt gttcgtgccg gtgcaggtta
    21721 tgacaatatt gatcttgatg ctgctacaaa agcgggtatc tgtgtaatga atactcctgg
    21781 acaaaattca aatgcagttg ccgaattggt ttttggtttg ttaatttatg cagtaagaaa
    21841 ttttttcaat ggcacttcgg ggaccgaatt aagagggaaa gaactgggca tccatgctta
    21901 tgggaatgta gggaaaaacg tagctcgtat agctaaaggt ttcgaaatga aactatatgc
    21961 ttttgatcct tattgtcagc ctgaagacat taaaaaagac ggtgttcagc ctttatcttc
    22021 tattgaatca ctttatgaaa attgtgaaat tatttctatt catacgcctg ccactgctga
    22081 aacaaaaaaa tctatcaatg ccaatttact tcaaaaatta ccgaaaggag cagtgattgt
    22141 gaatactgct cgaaaagaag taataaatga agaagattta atagcgtgca tggaaaaccg
    22201 attggatata aaatatgtaa cagatattat gcccgatcat catgcagaaa tggtagaaaa
    22261 atttccagga cgatatttct caactcctaa aaaaatgggc gctcagacag cagaagcaaa
    22321 cattaatgct ggaattgcag cagccaaaca aatagtagat ttctttaaaa atgggaacga
    22381 acaattcaga gtaaattaaa gaaaatagaa ataataattc ctgaatgaac gagagtttct
    22441 ggattattcc tctctttcag aatctaaa
//
LOCUS       DAOSAS010000009        21534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_1733_length_21534_cov_13.292332, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000009 DAOSAS010000000
VERSION     DAOSAS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21534
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1733_length_21534_cov_13.292332"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1702)
                     /gene="ilvB"
                     /locus_tag="PLR72_00935"
     CDS             complement(14..1702)
                     /gene="ilvB"
                     /locus_tag="PLR72_00935"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOK35809.1"
                     /translation="MKITGAKALMESLLQEGVNTIFGYPGGAILPVFDALYDYDKEIK
                     HILARHEQGAVHAAEGYARVSGKVGVSIITSGPAATNVITGISDAMLDSVPLVVITGQ
                     VGAGLLGSDAFQEIDVIGITQPITKWAYQIRRAEDVAWAIARAFYIAKSGRPGPVVLD
                     FTKNAQSEEVEFIYKPCKFIRSYIPVPELNPSQIEVAAQIINSAQKPLAIVGHGVIIG
                     EAETELKEFLEKADIPAAWTLLGASAMPTDFRLNMGFIGMHGNVAPNMKTNECDVLIA
                     IGMRFDDRVTGDLNTYAKQAKIIHIDIDAAEINKNVKVDAPVLASVKDALPALTKLIK
                     PAKHQEWIASFKEYADLEFEKVIQREIFPQSGKITMGEVIHKVSEATGKNAICVTDVG
                     QNQMAAARYFGFTKSRSLITSGGLGTMGFGLPAAIGAKIAAPDRTVCLFAGDGGFQMT
                     IQELGTIMEHQIGVKMIVLDNQFLGMVRQWQELFYEERYSFTRMKNPDFVAIGKAYNI
                     DGRWVTEREQLDEAIADMLKDDKPYLLAVKVEKKGMIYPMIPAGTTVTNIILGD"
     gene            complement(1776..3605)
                     /gene="ilvD"
                     /locus_tag="PLR72_00940"
     CDS             complement(1776..3605)
                     /gene="ilvD"
                     /locus_tag="PLR72_00940"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOK35810.1"
                     /translation="MEMELRSLTSTSGRRMAGARALWRAVGMKDEQMGKPIIAIVNSF
                     SQFVPGHVHLHEIGQKIKAEIEKLGCFAAEFNTIAIDDGIAMGHDGMLYSLPSRDLIA
                     DSVEYMMNAHKADAMICISNCDKITPGMLMAAMRLNIPAVFVSGGPMEAGELDGVQLD
                     LIDAMVQSADESISDEQIRRIEYAACPTCGSCSGMFTANSMNCLTEAIGLALPGNGTV
                     VATHVNREKLFEKAAHLIVENAFKYYRDNDDSVLPRSIATRAAFLNAMTLDIAMGGST
                     NTVLHLLAIANEAEVNFSMDDIDELSRHVPCICKVAPNTQKYHIQDVNRAGGVLGIMA
                     ELNRDGLIDTSVHRVDGLTLEQALAKYDIMLPNVEDEIIQLYKSAPANRFNLTLGSQN
                     FYYEELDRDRINGCIRDLVHAYTRDGGLAILKGNIAEDGCVIKTAGVDESILKFIGPA
                     KVFHSQEAACDGILSGKVQAGDVVVISYEGPKGGPGMQEMLYPTSYIKSRHLGKECAL
                     ITDGRFSGGTSGLSIGHISPEAAAGGNIALIQDGDIIEIDVPQRTIRVKLNDEELHLR
                     REKELSKGKNAFQPINRQRSVSKALKAYASMVSSADKGAIRII"
     gene            4068..6038
                     /gene="dxs"
                     /locus_tag="PLR72_00945"
     CDS             4068..6038
                     /gene="dxs"
                     /locus_tag="PLR72_00945"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOK35811.1"
                     /translation="MTEKPLQYLPKINSPDDLKKLDKEELKTVCEELRTFIINELSKH
                     PGHLASSLGTIELTVALHYVFNSPYDRIVWDVGHQAYAHKILTGRRDKFHTNRQFKGI
                     SGFPSPKESKYDAFGTGHASTSISAALGMDVASHLKNEKQRKIVAVIGDGALTGGLAF
                     EGLNNASMTKNDLLIILNDNQIAIDPLKGGINQYLVDITTSATYNKIRYKIYLLLKRL
                     DLIDESKKKNIIRFNNALKAILTRRHNLFEGLNIRYFGPVDGHDVEELVRILTEIKNY
                     KGPKVLHCITKKGKGYEPAEKAVTEWHSPGKFDIETGERNISNGGKKEPPLFQDVFGE
                     TLLELAKENNKIVGVSPAMLTGCSMTIMQKEFPDRVFDVGIAEGHAVTFSAGMAKEGL
                     IPFCNVYSSFLQRAYDNIIHDVALQNLHVVLCLDRAGLVGPDGPTHHGVFDLAYLRCI
                     PNLTIAAPRNEIELRNLMYTAQQEDSGPFAIRYPKGEGNIIDWKKPMNILPIGKGECL
                     KKGKDLAILSVGTIAANVAKAIEKVENKHRVSIAHYDMRYVKPLDEDLLHEIGEKFKQ
                     IITIEDGVIQGGFGSAISEFFSNSNDRPKIQRLGIPDTFVEQGSIEELQTLLGLDAES
                     IAKTIVEILKVNGKEKTKRKLKPNIMFGKKII"
     gene            6126..7460
                     /gene="trkA"
                     /locus_tag="PLR72_00950"
     CDS             6126..7460
                     /gene="trkA"
                     /locus_tag="PLR72_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOK35812.1"
                     /translation="MKIIIVGAGEVGTHLAKLLARENMEITLLDENPERLKDLDLNYD
                     LLTYEGSPTSINNLKEIGVENADLFIGVTPYESVNMTACMIATNLGAVKTLARINNYE
                     YLLPQNKEFFKKLGVDYLIYPEVLAANEIVESLKTSWLRQNMSFCNNALILLGIKVRS
                     NAVIINKKFSTGFFDHNRYRVVAIKRGSQTIIPKGSDEILANDIVYFITTPENLEFVK
                     EQAGKEDFPIRNVMFMGGSRIAQKTLFNLPSHIHAKVLERDKEKSLLLANKFPNALII
                     NADARNIDIIKQEGIEEADAFVAATSNSEANILACLVAKQFGVKKTIAEVENLDYISL
                     AESLDIGAVINKKMIAASYIYQITLHANVLNVRNLTAADAEVVEFVAKPTSKITRSKI
                     KDLKLPENVNIGGIVRNGIGSIVNGDTIIKPDDHVIVFCISSSIRKIEPFFN"
     gene            7546..9015
                     /locus_tag="PLR72_00955"
     CDS             7546..9015
                     /locus_tag="PLR72_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017140927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOK35813.1"
                     /translation="MNKEINVKLVMRMLGSLLLSEATALLLVFFLSFVFKESDGYYFL
                     ITSGVCYLIGLLGILLGRNASQEMGKREGSLVVTLAWVIFSLFGALPFFISKNIPSYT
                     DAFFETISGFTTTGATILNNIEELSHTMLFWRSFTQWMGGLGIIVIFLALLPMFGTSG
                     AQLFAAEASGPTKDKIHPKITETAKRLFLIYFLLTFAETILLRIGGMNWFDAVCHSFT
                     TIATGGFSTKQNSIAYWNSPFIQYIIILFMILAGVNFSLYYFGFKNKFKKIKENEEFR
                     YFLIVLFSFAIIIFISLIDFSQPVSFHKIENSWRDALFTITSLMTTTGFTIVDYMQWA
                     PFAWIILLIVMLTGASAGSTAGGIKMIRIVISFKFCYYEFKRLIHPNAVVPVRYSGHL
                     VQEDVITRVLAFVLLYLIVAIFGILILCVSGVGFIESIGALISCLSCVGPGLGLVGPM
                     GNFAYLPVFSKWFLSFIMLLGRLELFTVLVLFTPAFWKK"
     gene            9161..12049
                     /locus_tag="PLR72_00960"
     CDS             9161..12049
                     /locus_tag="PLR72_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOK35814.1"
                     /translation="MSSFLYRIAEIYYSQYQEKISEFTFVFPNRRAGIFFQHYISEIA
                     QKPIFSPEILTIEECFSSASSLLTADQISLIFLLYQIYRDISKSNESFDTFVFWGEML
                     LNDFDEIDKHLVNPQQLFTNVTELKQIDQQFKTFSEEQIKAIKQFWKNFIPFPAEKVQ
                     ENFISIWKILYLLYKEFRSALFKKSLATEGMIYRWVAEKMLNGEIILQWENKPFVFIG
                     FNALNACEKTLFSYLKNKNQADFYWDYEAAELRDENNPASRFYKENTQMFPSKFTLNS
                     QQETLSDKKIELIAVPSLVGQAKQTYELLDKLYPSDTSDHEWIKTAVVLPNETLLTPL
                     LYSLPPQIKKVNVTMGLPLNITPVQGLLEHIFELQRQMRITDNRYLFYHQTVSNLLNH
                     QYITLLCGREAQEIQTTVIENNLIYVDADAFENDELLATIFTPQEDTKNFLTYLLQIL
                     VILQKKFQQISETSQSYQLEYNFTSQYYLTLRRLNEVIKENASAIEMSLDTLMRLICQ
                     ITASLTIPFVGEPLEGLQIMGVLETRGLDFDNLIITSFNEDFFPKKTTPNSFIPYNLR
                     RGFGLPTIEHQEAIFSYNFYRLIHHAKHIFLLYDSRTEGLQKGEVSRFLYQLQYHYKV
                     PFTTKNVLYDISFKNSPVLQIEKNASVLEKLKSFLNNQPENRALSASSINTYIDCPLQ
                     FYLTYVEGLKEADEVKETIEYDMFGTLFHSSMEFLYQPFKNKLVNSSDLDTILKDSAQ
                     IEIAIQKAFSEKYFNKREDYQNIKLEGNYLLVANVLKKYIIQLLKIDKFYTPFKYLGS
                     EKEFQLEFPIQNGQEKVNLKGIIDRIDEKEGRLRIIDYKTGNGSLTFKNIEEVFAHEA
                     NNRPKYILQTFLYSMLYKYKSNTSLPIQPEIYYIKNLFKNDFTSQLTLKPNKEETIII
                     EDFNFYEEEFKTYLITCLEEIFNPAISFYQATNNKLCQYCPYQSICDR"
     gene            12132..12617
                     /locus_tag="PLR72_00965"
     CDS             12132..12617
                     /locus_tag="PLR72_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOK35815.1"
                     /translation="MGKEIERKYLVKGDFKPWIKNSYTIIQGYLCSVPERTVRIRVKN
                     QEKGFITIKGKTEKNGLNRYEWEKEIPLQDAQELLQLCEPGIIEKTRYEVIFKGQRFE
                     IDEFHGENEGLIIAEIELESENTPIQKPDWLGKEVTGEERFYNAQLRKNPYKNWENKD
                     F"
     gene            12718..13482
                     /locus_tag="PLR72_00970"
     CDS             12718..13482
                     /locus_tag="PLR72_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOK35816.1"
                     /translation="MKKLFFLMVSCLIFIPLSAQNELFLKKEFIYKGDTLPYRILFPE
                     NYDKTKSYPLVLFLHGAGERGKDNEKQLVHGSSLFTDAQNRENYPAIVVFPQCPENDF
                     WAPVHQEGKDFAFLNTKKPTPSMQLVIRLLNKITKEEAVDKNRIYVLGLSMGGMGTFD
                     LICRQPKKFAAAIPICGGVSLERLKKVRHMPIRIYHGGSDDVVSPEHSRNAFIELKAN
                     GSQKVEYIEFPGVGHNSWDYAFAQPDFLSWLFKQHK"
     gene            13681..13905
                     /locus_tag="PLR72_00975"
     CDS             13681..13905
                     /locus_tag="PLR72_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOK35817.1"
                     /translation="MAKIKGAVVIDIERCKGCNLCVVACPPRCLSLSKEANSKGYNFS
                     QLTNPEACVGCVACAYVCPDACITVYKMKV"
     gene            13935..15038
                     /locus_tag="PLR72_00980"
     CDS             13935..15038
                     /locus_tag="PLR72_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOK35818.1"
                     /translation="MDNKNKQNNEEVLLMKGNEALAHAAVRCGCDAYFGYPITPQSEV
                     METLMELKPWETTGMIVLQAESEVAVINMVYGAAGCGKKAMTSSSSPGVSLMQEGISY
                     MTSAELPAVIVNVMRGGPGLGTIQASQGDYFQAVKGGGHGDYKLITLAPASVQEMADF
                     IGLAFDLAFQYRTPVMMLADGIIGQMMEKVVLPPFKPRHTEAEIREKYPWATLGKTPD
                     RKPNIITSLELQSELMEAINLRLQSRYREIEKNEVRYEEMKTNDAEYLMVAFGTCARV
                     CQKVVEMARAEGIKAGLLRPITLYPFPTNIINNLSNSLKAILTVEMNAGQMVEDVRLA
                     VNGHIPVNFYGRMGGIVPSPDEVLDQLKKMMLP"
     gene            15094..15855
                     /locus_tag="PLR72_00985"
     CDS             15094..15855
                     /locus_tag="PLR72_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOK35819.1"
                     /translation="MTINEIIKPENLVYQKPKVLNDTPMHYCPGCSHGLVHRLIAEVI
                     EEMDIQDKTIGIAPVGCAVFAYNYLDIDWQEAAHGRAPAVATAIKRLLPDRYVFTYQG
                     DGDLAAIGTAETIHACNRGENIVIIFINNAIYGMTGGQMAPTTLEGMVTSTTPYGRNI
                     ALNGYPLNITNLLAQLPGTCYVTRQSVHTVATVRKTKKAIQKAFENSMMNKGTSVVEV
                     VSTCNSGWKMTPEQANEWMEKNMFPEYPLGDLKNI"
     gene            15928..16482
                     /locus_tag="PLR72_00990"
     CDS             15928..16482
                     /locus_tag="PLR72_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOK35820.1"
                     /translation="MKEEIIISGFGGQGVLSMGKILAYSGLMQGMEVSWMPSYGPEQR
                     GGTANVTVILSDEKISSPVLNSYDTAIILNQPSLEKFESKVKPGGILIYDGNNFHKFP
                     TRKDINIYRIDAIEYAYAHDMAKTLNMIILGGYLKIHPIVKMENVLKGLEKSLPERYH
                     HLIPLNEKAIQTGMEIIQLEHKVE"
     gene            complement(16670..18499)
                     /locus_tag="PLR72_00995"
     CDS             complement(16670..18499)
                     /locus_tag="PLR72_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOK35821.1"
                     /translation="MHYTFYDFLILIGSLGIFLYGMKVMSEGLQKIAGERLRAIMTAM
                     TKNRFSGVLTGLLITSIIQSSSATTVMVVSFVNAGLLTLYQAITVIMGANIGTTVTAW
                     VISLFGFKVNIASFAVPLIGLSIPFIFSSKSRRKSIGEFLIGFAFLFMGLEMLKNSVP
                     DIQSNPEILAFLSKYTNYGFGSVLLFLLIGTILTIIAQSSSAIMAITLIMCSKGWLSF
                     ELGAAMVLGENIGTTITANLAAIPANVSAKRAALAHLIFNVFGVIWILIFFYPFINLI
                     SYIITNFGPGDPNLLTSFTTSLDPETLKVISSDTTALTSEQLTLKQQLLQYQLATSYG
                     LSLFHTLFNLINTCLLIWFVNFIAKVVSFIIKQKDSDEEFQLKYISIGLLSTSELSIL
                     QAWKEIKVYVDRAQRMFQMVRELYYEKNEDEFVKKFSRIQKYENISDRMEIEIAHYLT
                     KVAEGRLSEKSKFEIQTMLRIISEIESIGDSCYNLARILMRKREDKAIYTPEMDENVN
                     RMMDLVEKTLTEMKKNVENNQATDQELAKIQNLENEINNLRDRLKFQNIDDVKEQKYD
                     YLASVTYMDIIAECEKMGDFIINIVEALNEATNKKDKIPLLQL"
     gene            18630..>19685
                     /locus_tag="PLR72_01000"
     CDS             18630..>19685
                     /locus_tag="PLR72_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOK35822.1"
                     /translation="MDKKVTIYCKNTRSFHDFPLGTSLLEIYQSLNIKLKYQVVAARV
                     NYKVEDLNFLVYKPKDIDFIDLSTSSGMRVYVRTLTMVIAKALSELYPYGVFRIEHPI
                     SKGYYCTINNLNEPVTSVLLSQIKERVNLIISQAKPIIVEEKQTQEVKRLFAEQQYQK
                     DKITLFDTLGIPYCKFYRIDDYIDYYNGVLLPSTDYLSLYDLIPYYDGLLLRVPNREN
                     PTELEEIIPQPKLYEIFKEYIEWNKIMNLSNVGEFNIACLNKQSFTLIKVSEALHEKK
                     VAAIADMIAQRGDKVKFVLVSGPSSSGKTTFSKRLSIQLMVCHFKPIIISLDNYFVDR
                     EKTPRDENGQLDFEHLHA"
     assembly_gap    19686..19785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19786..20064
                     /locus_tag="PLR72_01005"
     CDS             <19786..20064
                     /locus_tag="PLR72_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOK35823.1"
                     /translation="ARWESVRKGEEKWIFPFQENADIMFNSALLFEFAVLKRYAEPLL
                     SEIPKYCDEYTEAHRLLKFLSYFVAIPDREIPPTSLLREFVGGSSFRY"
     gene            20269..20910
                     /locus_tag="PLR72_01010"
     CDS             20269..20910
                     /locus_tag="PLR72_01010"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOK35824.1"
                     /translation="MAYCFGDVGGSYQEQFLNGINDWDISNTRYLVSLGLRQSFKNDL
                     GYKINVLYGSFVGDDANSRNEMRDYSYKNKVVGFSLQAEYVILGGFDPYKLNLHTLYI
                     YLGGGILNSDSKLWRKGLSISETPSDRPYDKVKLTDTTPIIPVGIGYQYHLTSNFSIG
                     AELGWIYSFSDYLDGIITQYSKRKDTLANLNITFTYKFKDTLTLPCHCLSYEK"
     gene            21159..>21534
                     /locus_tag="PLR72_01015"
     CDS             21159..>21534
                     /locus_tag="PLR72_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOK35825.1"
                     /translation="MLPILDEYHYYLKLEKGLSDNTIDAYERDLSKLSQYLSEAHIPL
                     EQATTEHLRDFLIEIRDLGIHPRSQARIISGITSFYHFLIYKNIITEDPTELLESLKI
                     GLRLPEVLSVSEINAMVAAIDLS"
BASE COUNT         7117 a         3494 c         3644 g         7179 t
ORIGIN      
        1 aatagaattt atttcaatct cctaagataa tattagtaac agttgttcct gcaggtatca
       61 ttggatagat cattcctttc ttttctactt ttactgccaa taaatagggt ttatcatctt
      121 ttagcatatc ggctattgct tcatctaact gttctcgttc agtcacccat cttccatcta
      181 tattgtaagc tttcccaatt gctacaaagt caggattttt cattctggta aaggaatatc
      241 tttcttcata gaataattct tgccattgac gtaccattcc cagaaattga ttgtctaaaa
      301 cgatcatttt tacaccaatt tgatgttcca taatagttcc cagttcctgg atagtcatct
      361 gaaatccgcc atcgccagca aaaaggcaaa cagtacggtc tggagcagct atttttgcac
      421 caatagcagc cggcagtccg aaacccattg ttcctaatcc tccggaagta attaagcttc
      481 gacttttcgt aaacccaaaa taccgagcag ccgccatctg gttttgcccc acatcggtaa
      541 cgcaaatggc atttttccct gtagcttctg aaactttatg aattacttct cccattgtta
      601 ttttgcccga ttgagggaaa atttcacgtt gaataacttt ttcaaattca agatcagcat
      661 attctttaaa agaagcaatc cattcttgat gtttggctgg ttttattaat tttgtcaaag
      721 ctggcaatgc atctttaacc gaagctaaaa caggagcatc tactttcaca tttttgttta
      781 tttcagcagc atcgatatca atatgaataa tttttgcctg ttttgcataa gtattcaaat
      841 cgcctgttac tcgatcatca aaacgcattc ctatggcaat taaaacatca cattcattgg
      901 ttttcatgtt tggagcaaca ttcccatgca tccctataaa tcccatgttt aaacgaaaat
      961 cagtaggcat ggctgaagca cctaataagg tccatgccgc tggaatgtct gctttttcca
     1021 gaaattcctt taattctgtt tctgcttctc ctataattac accgtgtccg acaattgcta
     1081 acggtttttg agctgaatta ataatttgtg ctgcaacttc gatttgagaa ggattcagtt
     1141 ctggaacagg tatgtaactg cggatgaatt tgcaaggttt ataaataaat tctacttctt
     1201 cagactgagc attttttgta aaatcaagaa ccactggtcc tggacgtcca cttttggcaa
     1261 tataaaaagc acgagctatc gcccacgcga catcttcagc tcttcgaatc tgataagccc
     1321 atttggtaat aggctgagtg attccaatga cgtcgatttc ctggaaagca tcgctgccta
     1381 ataatcctgc accaacttga ccggtaatga ctaccagcgg aacactatcc agcattgcat
     1441 cgcttatgcc cgtaataaca ttggtagctg caggacctga agtaataatg gatacaccaa
     1501 ctttacctga tacacgagca tagccttcag cagcatgtac tgcaccctgt tcatggcgtg
     1561 ccagaatatg tttgatttcc ttatcgtaat catacaaagc atcaaaaact ggcaatatcg
     1621 ctcctccggg ataaccaaaa atagtattta ctccttcttg caataatgat tccattaatg
     1681 cttttgcacc tgtaattttc ataaatcaat ttttattatt cactcctata agaacaaagg
     1741 ttattttttt ctaatcctta tatttttaaa aagtttcaaa tgattcttat tgcaccttta
     1801 tcagcagagc taaccattga agcataagct tttaaggctt tagaaactga acgttgacgg
     1861 tttattggtt gaaaagcatt tttacctttt gataattctt tttcacgacg taaatgtaat
     1921 tcttcatcgt tcaatttgac tcgtatcgta cgttgaggta catcgatttc aataatatct
     1981 ccatcttgaa ttagtgcaat attccctcct gcagcagctt caggagaaat atgaccgata
     2041 gataatcctg atgttccgcc agaaaaacgt ccgtcggtaa ttaaagcaca ttctttcccc
     2101 aagtgacgtg atttgatata agacgtcggg taaagcattt cttgcattcc cggacctcct
     2161 tttggacctt cataactgat cactaccaca tcacctgcct gtacttttcc tgataaaatt
     2221 ccatcacaag ctgcctcttg agaatgaaaa actttagcag gtccaataaa ttttaaaata
     2281 ctttcatcta ctcccgctgt ttttattacg caaccatctt ctgcaatatt tccttttagt
     2341 attgccaatc cgccatctct ggtataagcg tgaactaaat ctcgaatgca accatttatt
     2401 ctgtctctat caagttcttc ataataaaaa ttttgtgagc caagtgttaa attgaaacga
     2461 ttggctggtg cacttttata aagttgaata atttcatcct ccacatttgg taacataata
     2521 tcgtactttg ctaatgcttg ctcgagtgtt aaaccatcca ctcggtgaac agatgtatca
     2581 atcaaaccat cacggttgag ctctgccatg attcctaaca cgcctccagc acggttaaca
     2641 tcttgtatat gatacttttg cgtattagga gccactttac aaatacatgg aacatggcga
     2701 gaaagctcat caatatcatc catcgaaaaa ttaacctctg cttcattggc aattgctaaa
     2761 agatgaagga ctgtattggt tgaacctccc atagcaatat ccagggtcat agcattaaga
     2821 aaagctgcac gtgtcgctat ggaacgaggc aaaacagaat catcattatc gcgataatat
     2881 ttaaaagcat tctcaacaat taaatgagct gccttttcaa acaatttttc tcgattgaca
     2941 tgagttgcta caactgtgcc attccccggc aaagcaaggc caatagcttc tgttaaacaa
     3001 ttcattgaat tggcagtaaa catacctgaa caagagccac aagtaggaca agctgcatat
     3061 tcaattcgcc gaatttgttc atcagaaata ctttcatcag ccgattgaac catagcatca
     3121 attaaatcaa gttgaacacc atctaattct cctgcttcca ttggtccgcc agaaacaaat
     3181 acagcgggta tattcagccg catggcagcc attaacattc caggtgtaat tttatcacaa
     3241 ttactaatac aaatcattgc atcagctttg tgtgcattca tcatatattc cacgctatca
     3301 gcaattaagt cgcgcgaagg taaagaatac aacataccat catgccccat tgcaatgcca
     3361 tcatcaatgg ctatggtatt aaattcagca gcaaaacatc ctaatttttc tatttcagct
     3421 ttaatttttt gtcctatctc atgtaaatga acatgaccag gcacaaactg gctaaatgaa
     3481 tttacaatag caataatagg tttacccatt tgttcatctt tcatacctac agctcgccat
     3541 agagcccgtg ctcctgccat tcttcgtcca ctggtacttg ttaaacttcg taattccatt
     3601 tcaatatttt tttattatct ataaagaaaa accttcctca ttgtttggga ggaaggttaa
     3661 cattattttt tgtttatgat tgtaaaccat atagaaccat cctcaccttt gatttttcac
     3721 caaaagtagc acgacgattc tattgatatt tctatttgct ctgaacattt tctctttatt
     3781 tctgtttgca aagataaact ttgaaattga aaaaacaaat agtttacaaa aaaattttct
     3841 tttttaatca aaagaattcc tcgaggcttg cctcggggtt attcattatt ataaaaaaat
     3901 aggttgaaag taaaaaatca tttttagtta aattgataac ttatgatttc atataattgt
     3961 ttataaaaaa tttaaacgta aatttgtaat aggataaaaa ataataatta ttaggtagct
     4021 aacgaataaa ataatgtatt atttttgtgg gcaattttaa aactataatg acagaaaaac
     4081 ctttacaata tttaccaaag ataaattctc cagatgattt aaaaaaactt gataaagaag
     4141 aacttaaaac tgtctgtgaa gaattaagaa cttttattat caatgaactt tcgaaacatc
     4201 ctggtcatct ggcttccagt ttaggaacca ttgaacttac tgttgccctt cattacgttt
     4261 ttaacagccc ttatgataga atagtgtggg atgtaggtca tcaggcttat gctcataaaa
     4321 tacttacggg ccgacgggat aaatttcata caaaccgtca attcaaaggt attagtggtt
     4381 tcccgagtcc aaaagaaagt aaatatgatg ctttcggaac tggtcatgct tccacttcta
     4441 tttctgcagc attaggaatg gatgtggctt cacatttaaa aaacgaaaaa caaagaaaaa
     4501 ttgtagcagt tattggcgat ggagctttaa cggggggatt ggcttttgaa ggattgaata
     4561 atgcttccat gactaaaaat gatttattga ttatccttaa tgataatcaa attgctattg
     4621 atcctttgaa aggtggaata aatcaatatt tagtagatat tactacttct gctacctata
     4681 ataaaattcg atataaaatt tatctacttt taaaaagatt agatttaatt gatgaatcga
     4741 aaaagaaaaa cattattcga tttaataatg ctttaaaagc tattctaact cgacgacata
     4801 atttatttga aggtttaaac atacgttact ttggtcctgt tgacgggcat gatgtggaag
     4861 aattggttcg gattttaaca gaaataaaaa actacaaagg tcctaaagta ttgcattgca
     4921 ttactaagaa agggaaagga tacgaacctg cagaaaaggc tgtaaccgaa tggcattctc
     4981 ctggaaaatt tgatattgaa acaggtgaaa gaaatatatc aaatggaggg aaaaaagaac
     5041 caccactttt ccaagatgtt tttggtgaaa ctttattaga attagctaaa gagaataata
     5101 agattgtagg cgtttctccg gctatgctta cgggatgttc tatgaccatt atgcaaaagg
     5161 aatttcctga tagagttttt gatgtgggaa tagcagaggg tcatgcggta actttttcag
     5221 caggaatggc caaagaagga ttgattccgt tttgtaatgt ttattcatct tttttgcaaa
     5281 gagcttacga taatattatt catgatgttg ctcttcaaaa tttgcatgtg gttttatgcc
     5341 tcgatagagc tgggctggta ggaccagatg gtcctactca tcacggggtt tttgatttgg
     5401 cttatctccg atgcatccct aatttaacaa ttgctgcccc tcgtaatgaa attgaactcc
     5461 gtaatttaat gtacactgca caacaagaag attcaggacc ttttgctatt cgctatccaa
     5521 aaggagaagg aaatattatt gattggaaaa aaccaatgaa tatcttaccg attggaaaag
     5581 gtgaatgctt gaaaaaaggg aaagatttag ccattttatc agtaggaacg atagcagcca
     5641 acgtagcaaa ggctatcgaa aaagtcgaaa acaaacatcg tgtttccatt gctcattatg
     5701 acatgcgtta tgtaaaacct ttagatgaag atttactaca cgaaattgga gaaaaattta
     5761 aacaaatcat aaccattgaa gatggagtga tacaaggagg ttttggaagc gccatttcag
     5821 aatttttcag taatagtaat gataggccga aaatccaacg gctgggaatt cctgatacct
     5881 ttgttgagca aggaagcata gaagaattgc aaactttgtt aggcttggat gctgaaagca
     5941 ttgccaaaac tattgttgaa attctgaaag tcaatggaaa agaaaaaaca aaacgaaaat
     6001 tgaaaccaaa tataatgttt ggaaaaaaga taatttgaaa gcaaagattt acgataaaat
     6061 gattttttaa aaattaaacc tttaattttt cagtagctta tttctctcct attaaattta
     6121 caaaaatgaa aattattatt gttggagcag gtgaagttgg tacccatttg gcaaaattgt
     6181 tagcgcggga aaatatggaa atcactttgc tcgacgagaa tccagaacga ttaaaagact
     6241 tagatttgaa ctacgatctt ttgacctatg aaggatcacc aacttcaatt aataacttaa
     6301 aggaaattgg cgttgaaaat gctgatcttt tcattggcgt cactccttat gaaagtgtga
     6361 atatgactgc ctgtatgata gctaccaatt taggtgctgt aaaaacattg gcaagaatca
     6421 ataattacga atatttatta ccacaaaata aagaattttt caaaaagttg ggggttgatt
     6481 atttaattta tccagaagtg ttagctgcca atgaaattgt agaatctttg aaaacaagtt
     6541 ggttgcgtca aaacatgagt ttttgcaata atgctcttat ccttttaggc ataaaagttc
     6601 gcagtaatgc tgttatcata aataaaaaat ttagcacagg cttttttgat cataaccgtt
     6661 acagagttgt tgccatcaaa cgaggaagtc aaacaatcat tccaaaaggt tccgatgaaa
     6721 tactcgctaa tgatattgtt tatttcatca caactcctga gaatctggaa tttgtaaaag
     6781 aacaagcagg gaaagaagat tttccaattc gaaatgtaat gtttatggga ggtagccgaa
     6841 ttgcacaaaa aaccttattc aatcttcctt cccatatcca tgccaaagtg ctcgaaagag
     6901 ataaagaaaa gagtttgttg ttggcgaaca agtttcctaa tgcattaatt attaatgctg
     6961 atgccagaaa tattgatatt attaaacaag aaggaattga agaagcagat gcttttgtgg
     7021 cagcaacttc caattctgaa gccaatattt tagcttgttt ggtagcaaag caatttggag
     7081 taaaaaaaac tattgcagag gttgaaaatc tggattacat ttctctggct gaaagtttgg
     7141 atattggtgc agtaattaac aaaaagatga ttgctgccag ttatatttat caaattacct
     7201 tacatgcaaa tgtgctcaat gttcgcaatt taacagcagc agatgcagaa gtagtggaat
     7261 ttgtggctaa accaacatca aaaatcaccc gttcaaaaat taaagattta aaattacctg
     7321 aaaatgtaaa tattggggga attgttagaa atggcatagg cagtattgtc aatggtgata
     7381 ctattatcaa accggatgat catgtgattg tattttgtat atcttcatct attcgaaaaa
     7441 ttgaaccctt ttttaactaa tagagcttta gataatatat tttcccacac tgttgaaaaa
     7501 agtttaacat gagataataa acttaaatag ctaaattcca agactatgaa taaagaaatc
     7561 aatgtgaagt tagtaatgcg gatgctggga agtttattat taagtgaagc gactgctctt
     7621 cttcttgttt tctttctttc atttgttttt aaggaaagcg atggctatta ttttcttata
     7681 acttctggtg tttgttattt aattggtctt ttaggaatat tattaggacg aaatgcttct
     7741 caggaaatgg ggaagcggga gggttcattg gtggtaacac ttgcctgggt tattttttcc
     7801 ttgtttggtg ctttgccttt ctttattagt aaaaatattc cttcttacac tgatgctttc
     7861 tttgaaacaa tttccggttt tactactaca ggtgctacta ttttaaacaa tattgaggaa
     7921 ttatcacata ctatgttgtt ttggagaagt ttcactcagt ggatgggtgg gctgggaata
     7981 attgttattt ttttggctct tttacccatg tttggaacaa gtggggctca actttttgca
     8041 gcagaagcaa gcggaccaac caaagataaa attcatccaa aaattactga aaccgccaaa
     8101 cgattgtttt taatttactt tttattgact tttgctgaaa caattttact ccgtattgga
     8161 gggatgaatt ggttcgatgc tgtttgccat tcatttacca ctatcgcaac tggtggcttt
     8221 tctaccaaac aaaacagtat agcgtattgg aattctccat ttattcaata catcatcata
     8281 ttatttatga tacttgcagg tgtgaatttt agcttgtatt attttggttt taaaaataaa
     8341 tttaaaaaga taaaagaaaa tgaagaattc cgttattttt taatcgttct tttttctttt
     8401 gccattatta tttttatttc tttaatcgat tttagccaac ctgtttcttt tcataaaata
     8461 gaaaattctt ggagagatgc tttatttact attacttcgt tgatgactac tacaggattt
     8521 actattgttg actatatgca atgggcacct tttgcgtgga ttattttgtt aattgtaatg
     8581 ctgacaggtg cttctgcggg ttctactgca ggtggcatta aaatgataag aattgtaata
     8641 tcgtttaaat tctgctatta tgaatttaaa cgattaattc atcccaatgc tgtggttccc
     8701 gtgcgttata gtggtcattt ggtacaggaa gatgttatta cacgagtttt ggcttttgtc
     8761 ttgttatatc tgattgtggc tatttttggg attttgattt tgtgcgtctc cggagttggt
     8821 tttatagaat ccattggagc attaatttct tgtcttagct gtgttggacc cggattagga
     8881 ttggttggtc ctatggggaa ttttgcttat ttaccagttt ttagcaaatg gtttttatca
     8941 tttattatgt tgctgggacg tttggaactt ttcactgtat tagttctttt tactcctgcc
     9001 ttctggaaaa aataagattt ttctctattt atttgatttt ttcaaaacca ttgtttgaaa
     9061 aacacggaaa gacgctctat caattataaa attacaaatt ttaaatgaga aagttttaat
     9121 tttatatttt tactgttttt ttatccttaa aaaattataa atgtcttcat tcctttatcg
     9181 catagcagaa atttattatt ctcaatatca ggaaaaaatc agtgaattta cttttgtgtt
     9241 ccccaaccgt agagcgggta ttttttttca gcattatatt tcggaaatag cacaaaagcc
     9301 aattttttca cctgaaatac ttactatcga agaatgtttt tcttcagcgt cttcacttct
     9361 aacagcagac caaatcagct tgattttttt actttatcaa atttatcgtg acatcagcaa
     9421 atcaaatgaa agttttgata cttttgtttt ctggggtgaa atgttactca acgattttga
     9481 tgaaattgat aaacatcttg ttaatcctca acaattattt accaatgtta ctgagctgaa
     9541 acaaattgat caacagttca aaactttttc tgaagagcaa ataaaagcta taaaacaatt
     9601 ttggaaaaac tttattcctt ttcctgctga aaaagttcag gaaaatttca tttctatctg
     9661 gaaaattctt tacctacttt acaaggaatt tcggtcagca ttattcaaaa aaagcctggc
     9721 tactgaagga atgatatatc gttgggtagc tgaaaaaatg ctaaatggtg aaattattct
     9781 tcaatgggaa aacaaaccgt ttgtttttat tggttttaat gctttaaatg cttgtgaaaa
     9841 gacacttttt tcatatttaa agaataaaaa tcaagctgat ttttattggg attatgaagc
     9901 tgctgaactt cgtgatgaga acaatcctgc ttctcgtttt tataaggaaa acacgcaaat
     9961 gtttccttca aaatttacac tgaattctca acaggaaact ttatcagata aaaaaatcga
    10021 gttaatagcc gttccttcgc tggtgggaca agcaaagcaa acctatgaat tgctcgacaa
    10081 actttaccct tcggatactt cggaccacga atggataaaa actgctgtcg ttttgcccaa
    10141 cgaaactttg ttgacgccat tgctttattc tttgccacca cagataaaaa aagtaaatgt
    10201 tacaatggga ttgccattaa acattacacc agtgcaaggg cttttggaac atatttttga
    10261 attacagcga caaatgcgta tcactgataa tcgatattta ttttatcatc aaactgtttc
    10321 caatctttta aatcatcaat atattacgtt actttgcggt cgtgaggctc aagaaattca
    10381 aactactgta atagagaaca atttaattta tgttgatgca gacgcatttg aaaacgatga
    10441 actgctggca actatcttta ctcctcagga agacacgaaa aattttttga cttatttgtt
    10501 acagattctt gtcatccttc agaaaaaatt tcagcaaatt tcggaaactt ctcagagtta
    10561 tcagttagag tataatttta caagtcaata ttacctcact cttcgacgtt tgaatgaagt
    10621 aatcaaggaa aatgcttctg cgatagaaat gagcttggat actttgatgc gtttgatatg
    10681 tcaaattact gcttcactta ccattccatt tgttggtgag ccattggaag gcttacaaat
    10741 tatgggcgtt ctggaaacca gagggttgga ttttgataat ttaattatca cctcctttaa
    10801 tgaggatttt tttcctaaaa aaactacacc caatagcttt attccttata atctacgtcg
    10861 tggatttggt ttacctacca ttgaacatca agaagctatt ttttcctata atttttaccg
    10921 attgattcat catgcaaaac atattttttt actttacgat tcacgtacag aaggacttca
    10981 aaaaggagaa gtgagtcggt ttttgtatca attgcaatat cattacaaag ttccttttac
    11041 taccaaaaat gttttatacg atattagttt caaaaattca cctgttttac aaattgagaa
    11101 aaatgcttct gttcttgaaa aattgaaatc tttcctcaat aatcagcctg agaatcgtgc
    11161 tctttcggca agttctatca atacttatat tgactgtcca cttcaatttt atcttacgta
    11221 tgtagaagga ttaaaagaag ctgacgaagt aaaggaaact attgaatatg atatgtttgg
    11281 gacattgttt cacagttcga tggaatttct ttatcagcct tttaaaaata agttggtcaa
    11341 ttcttctgat ttagatacca tattaaaaga ttcggcacaa atcgaaattg ctattcagaa
    11401 agctttttca gaaaaatact ttaacaaaag agaagattat caaaatatta aattggaagg
    11461 taattatctt ttggtggcaa atgtgcttaa aaaatatatt attcagttat tgaaaatcga
    11521 caaattttat actcccttta aatatcttgg ctctgaaaag gaatttcaac ttgaattccc
    11581 catacaaaat ggacaagaaa aagtaaactt aaaaggcatc attgacagaa ttgacgaaaa
    11641 agaaggacgt ttaagaatca ttgattataa aacaggaaat ggctcattga cttttaaaaa
    11701 tatagaagaa gtttttgctc acgaggcaaa taatcgtcca aaatatatat tgcaaacttt
    11761 cttgtatagt atgttgtata aatacaaatc caacacttca ttaccaatac agccagaaat
    11821 atattacata aaaaatctgt ttaaaaatga ttttacttcg caattgactt tgaaacctaa
    11881 taaggaggaa actatcatta ttgaagattt taatttttac gaagaagaat ttaaaactta
    11941 tttaattact tgtttggaag aaatttttaa tccagccatt tctttttatc aagctacaaa
    12001 taataaactt tgtcaatatt gcccatatca gtctatttgc gacaggtagc aataagttaa
    12061 aaaaatctgg agtatcctta aatttatttc ataaatttgc attattataa tcaagttttc
    12121 tgttttttga aatgggaaaa gaaattgaac gtaaatattt ggtaaaaggt gatttcaagc
    12181 catggattaa aaatagttat actattattc agggatattt atgttctgta ccagaacgaa
    12241 ctgttcgtat tcgtgtcaaa aatcaagaaa aaggatttat taccatcaag ggaaaaacag
    12301 aaaaaaatgg attaaatcgt tatgaatggg aaaaagaaat tcctttgcaa gatgctcagg
    12361 aattgcttca gctatgcgaa ccagggatta ttgaaaaaac tcgttatgaa gtaattttta
    12421 aaggtcaacg ttttgaaatt gatgaatttc atggcgaaaa tgaaggactt atcattgccg
    12481 aaatagaatt ggaaagtgaa aacactccta ttcaaaaacc cgattggtta ggcaaagaag
    12541 taacaggaga ggaaagattt tataatgctc agttaagaaa aaatccttac aaaaactggg
    12601 aaaataaaga tttttaagca agtaaaggtt tactatatta tgatttttta ctaacattta
    12661 atcaaaagaa ttcctcgagg cttgtctcgg ggttattcat ttaataattt ttgtatgatg
    12721 aaaaaacttt tctttttaat ggtttcttgt ctgattttta ttcccttatc agctcaaaat
    12781 gaattatttc tgaaaaaaga atttatttat aaaggtgata cgttgcctta tcgtatttta
    12841 tttccagaaa actatgacaa aactaaatcc tatcctttgg ttcttttctt acacggagct
    12901 ggcgaacgag gaaaagataa tgaaaaacag cttgttcatg gatcgtcact ttttaccgat
    12961 gcccaaaaca gagaaaatta ccctgcaata gttgtttttc ctcaatgtcc tgaaaatgat
    13021 ttttgggcac ctgttcatca ggaagggaaa gattttgctt ttttaaatac caaaaagccg
    13081 acaccttcta tgcagcttgt tatccgtctt ctgaataaaa tcacaaaaga agaagctgtg
    13141 gataaaaacc gaatatatgt attagggctt tcgatgggag gaatgggaac ttttgattta
    13201 atttgcagac aacctaaaaa atttgcagca gctattccta tttgtggagg agtgagttta
    13261 gaacgactta aaaaagttcg tcatatgccc attcgtattt atcatggtgg cagcgatgat
    13321 gttgtctcac cagaacattc acgaaatgct tttattgagc taaaagccaa tggctctcaa
    13381 aaagtagaat atattgaatt cccaggcgta ggacataata gttgggatta tgcttttgca
    13441 caacctgatt ttttaagctg gctattcaaa caacataaat aacattgaaa ttgaatagtt
    13501 tagcttgtca ctttgttaaa taactcttac caaaaaagca atattgccac aattatcacc
    13561 ctgatttata aaatttgact aattttgcaa cgaaaaaatg ataagtatta aaagtttttt
    13621 ttaaaacaga aaaacttcag aatatatcat tagaaaatta aaagattaaa agatcaaaaa
    13681 atggcaaaaa ttaaaggtgc tgttgtaata gacattgaac ggtgtaaagg atgcaattta
    13741 tgtgtagttg cttgtcctcc tcgatgcctg tctttatcaa aagaagcaaa ttcaaaagga
    13801 tataattttt ctcaactaac taatcctgag gcatgcgtgg gttgtgtagc gtgtgcttac
    13861 gtttgccccg atgcctgtat cactgtatat aaaatgaaag tttaataatt tcttaatggt
    13921 aagataatat aaaaatggat aataaaaaca aacaaaataa tgaagaggtt ctgctaatga
    13981 aaggcaatga ggcacttgct catgctgctg tacgttgtgg ctgcgacgct tattttggat
    14041 atcctatcac tcctcaatcc gaagttatgg aaacgctcat ggaacttaaa ccatgggaaa
    14101 caacagggat gatagtttta caagctgaga gtgaagtagc tgttatcaat atggtatacg
    14161 gagctgctgg ttgcggaaag aaagccatga cttcctcttc gagtcctgga gtaagcctta
    14221 tgcaagaagg aatttcatac atgacatctg ctgaattacc ggctgtaata gttaatgtaa
    14281 tgcgcggcgg accaggttta ggaactattc aagcaagtca gggagattat tttcaggcag
    14341 taaaaggtgg agggcatggc gattataaac tgattacatt ggctccagct tctgtgcagg
    14401 agatggctga tttcattggt ttagcatttg atcttgcttt tcaatatcgt actcctgtca
    14461 tgatgcttgc cgatggaatt attggacaaa tgatggaaaa agtagttttg ccaccattca
    14521 agccacgtca tactgaagct gaaatcaggg aaaaatatcc atgggcaacg ttaggtaaaa
    14581 ccccagaccg caaacctaac attattactt cgctggaact tcagtctgaa ctaatggagg
    14641 ctattaatct tcgtcttcaa agtcgctacc gcgaaataga aaaaaacgaa gtacgctatg
    14701 aagaaatgaa aaccaacgat gcagaatatt tgatggtagc ttttggaaca tgtgctcgcg
    14761 tttgccagaa agttgttgaa atggcacgag ctgaaggaat taaagcaggt ttactgcgac
    14821 ccattacttt gtatcctttc ccaactaaca ttataaataa tttgtccaat tccttgaaag
    14881 ctatcttaac agtagaaatg aatgctggac aaatggttga agatgttcga ttggcggtca
    14941 atggacatat tcctgttaac ttttacggta gaatgggagg aattgttcct tctcctgatg
    15001 aagtgctgga tcagttaaaa aaaatgatgt taccttaaac tgcaagcagt caataaaaaa
    15061 gtttattaag ttgtaaatta attaaagtat aaaatgacaa taaatgagat aatcaagcca
    15121 gaaaatttgg tatatcaaaa accaaaagtt ttgaatgata cgccaatgca ttattgccca
    15181 ggttgcagtc atggcttggt gcacagactg attgctgaag taattgaaga aatggacatt
    15241 caagacaaaa ccattggaat tgctcctgta ggttgtgctg tctttgctta caattatctt
    15301 gatattgatt ggcaagaagc agctcatggt cgtgcaccag cagtggcaac agctattaaa
    15361 agattacttc ctgaccgtta tgtatttact tatcaaggag atggtgattt agcagccatt
    15421 ggtacagcag aaacaatcca tgcttgcaat cgaggagaaa atattgtaat catttttatc
    15481 aataacgcta tctatggcat gacaggtgga cagatggcac caactaccct tgagggaatg
    15541 gtgacttcta ccacacctta tggacggaat attgctttaa atggttatcc attgaacatt
    15601 actaatttgt tagctcaatt acccggaaca tgttacgtaa ctcgtcaaag tgtccatacg
    15661 gtagctacgg ttcgaaaaac aaaaaaagct attcaaaaag cttttgaaaa ttctatgatg
    15721 aacaagggaa catctgttgt tgaagttgtt tctacttgta attcaggatg gaaaatgaca
    15781 cctgaacagg caaatgaatg gatggagaaa aatatgtttc ctgaatatcc gctgggagat
    15841 ttaaaaaata tttaaatttt caattcaaaa aataaactaa ataattgaaa gtgataaagt
    15901 aatactttca ttgctaacat ttaaattatg aaagaagaaa taataatttc tggttttgga
    15961 ggacaagggg ttctttcaat gggtaaaata ttggcttatt ctggtttgat gcagggtatg
    16021 gaagtaagtt ggatgccttc ttacggacct gaacaacgtg gtgggacagc caatgtaact
    16081 gtaattttga gcgatgaaaa aatcagttct cctgtgctca attcatacga tactgctatt
    16141 attttaaatc aaccttcgtt agaaaaattt gaaagtaaag tgaaaccagg tggcattctt
    16201 atttatgatg gcaacaattt tcataaattc cctaccagaa aagacatcaa catttatcgc
    16261 attgatgcta ttgaatatgc ttatgctcac gatatggcta aaacattaaa tatgattatt
    16321 ttaggtggtt atttaaaaat acaccccatt gtaaaaatgg aaaatgttct aaaaggtttg
    16381 gaaaaatcac tgcctgaacg ttatcatcat ttaattcctt tgaatgaaaa agccatccaa
    16441 acaggaatgg aaattattca attggagcac aaagttgaat aaaaagttga tttttgataa
    16501 agtctcctta tcgccgataa ttcctcatga gtcggcgttt ttgttttatc gcaagttatc
    16561 atgcgaaatg aatttatcca gaccattagg aaaatttttt agggtaaaga tgagaacaaa
    16621 ataattcaaa ttaaaatttt tccattctta ttaattattc gaattcagct tataattgga
    16681 gtaatggaat tttatccttt ttattcgttg cttcgttcaa tgcttcaaca atattgataa
    16741 taaaatcgcc cattttttcg cattctgcaa ttatgtccat ataggtaaca cttgctaagt
    16801 aatcgtattt ttgttctttt acatcatcaa tattttgaaa tttaagccga tctcgaagat
    16861 tatttatttc attttccaaa ttttgaattt tagctaattc ctgatcggtt gcctgattgt
    16921 tttctacatt ttttttcatt tctgtgaggg ttttttcaac caaatccatc attctattta
    16981 cattctcatc catttcagga gtataaattg ctttgtcttc tctctttctc atgagtattc
    17041 tggcgaggtt gtaacaacta tctcctatgc tttcaatttc agaaataatt cgcaacatag
    17101 tttggatctc aaatttactt ttttcgctca atcgcccttc tgctaccttt gtcaaatagt
    17161 gagctatttc tatttccatc ctatcactaa tgttttcgta cttttgaatt ctgctgaact
    17221 ttttaacaaa ttcatcttca tttttttcat aatataattc tcgtaccatt tgaaacattc
    17281 tttgagctcg atcaacataa actttaatct ctttccatgc ttgaagaata gaaagttcag
    17341 aagttgaaag caatcctatt gatatatatt tcaattgaaa ttcttcatcc gaatcttttt
    17401 gtttgatgat aaacgataca accttagcaa taaaattaac aaaccatatt aaaaggcatg
    17461 tatttataag attaaatagc gtgtgaaaaa gagataatcc ataggaagtt gccagttgat
    17521 attgaagcaa ttgttgctta agggttagtt gttcggaagt gagtgctgtg gtatctgatg
    17581 aaattacttt taaagtttca gggtctaaac ttgtggtaaa agaggttaat aagtttggat
    17641 ctccaggccc gaagttagta atgatatagg aaattaaatt tataaaagga tagaaaaata
    17701 ttaatatcca aatcactcca aaaacgttaa atataagatg agccagagct gcacgtttag
    17761 ccgaaacatt agctggtatc gctgctaaat ttgcagtaat ggttgtacca atattttctc
    17821 cgagcaccat cgctgctccc agttcaaatg aaagccaacc tttgctgcac attatcaagg
    17881 taatagccat tatagcactt gaagattgcg caataatagt taaaatggta cctattaaaa
    17941 gaaataataa aactgaccca aatccatagt tggtatattt ggaaagaaat gctaaaattt
    18001 cagggttact ttgaatatcc ggaactgaat tttttaacat ttcaagtccc ataaacaaaa
    18061 acgcaaatcc gattaaaaat tcgcctatag atttgcgtct actttttgaa gaaaaaataa
    18121 aaggtattga aagtcctata aggggaaccg caaaagaagc aatgtttact ttaaagccaa
    18181 ataaagaaat aacccatgca gtaactgttg taccaatatt agcccccata attacagtaa
    18241 tagcttgata tagtgtaagt aaacctgcat ttacaaagct tactaccatt actgttgttg
    18301 cggacgaaga ttgaattatg gaggtaatca ataagcctgt taaaactcca ctaaaacggt
    18361 ttttggtcat tgcagtcata atagcacgaa gacgttcgcc cgctattttt tgtaatccct
    18421 cgctcattac cttcatccca taaaggaata tacccaacga gccaatcaga atcaaaaaat
    18481 cataaaatgt ataatgcatt atataatcat ttttttaaga tttcactaca aagttattca
    18541 aattttgaat tcttaaaagt aaagaaataa aaaaagatgt atatttacaa tcaaaatgaa
    18601 aatacatttt atattaagaa gtttaaatta tggataaaaa agtaactatc tattgcaaaa
    18661 atacgcgttc gtttcatgat tttcctttgg gtacctcttt acttgaaatt tatcaatctt
    18721 taaatataaa attaaaatat caggtagttg cagctcgtgt caattataaa gttgaagatc
    18781 ttaatttttt ggtatataaa cccaaagata ttgattttat tgatttgagc acttcttcag
    18841 gaatgcgggt atatgtccga acgctaacaa tggtaattgc aaaagctctt tcagaattat
    18901 acccttatgg tgttttccga attgaacatc ctatttccaa aggatattac tgcactatta
    18961 acaatttaaa tgaacctgtc acatcagtct tgctttctca aatcaaagaa cgagtaaatt
    19021 taattatttc tcaagctaaa cccattatcg tagaagaaaa acagacacaa gaagtaaaga
    19081 gattatttgc tgagcaacaa tatcagaaag ataaaattac tctttttgat acgttgggca
    19141 ttccttattg taaattttat cgaatagatg attatataga ttattataat ggtgttttat
    19201 taccttcaac tgactattta agtttatacg atttaatacc ttattatgat ggtttattgc
    19261 ttcgtgttcc caacagagaa aatccaaccg aattagaaga aattattcca caaccaaaat
    19321 tatatgagat atttaaagag tatattgaat ggaataaaat aatgaatttg agtaatgtag
    19381 gggaatttaa cattgcatgt ttaaacaaac aatcttttac tttgataaag gtttcagaag
    19441 ctttgcatga aaaaaaagta gcagcgatcg ctgatatgat tgctcagcga ggtgataaag
    19501 taaagtttgt attggtctca ggcccttctt cttcaggaaa aacgactttt agcaagagat
    19561 tatctattca attaatggtt tgccatttca aacctattat catttctctt gataattatt
    19621 ttgtagatcg agaaaaaaca ccgcgggatg aaaatggaca actggatttt gaacatttgc
    19681 atgcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngctcg ctgggaaagt
    19801 gtaaggaaag gggaagaaaa atggattttc ccttttcagg aaaatgcaga cattatgttt
    19861 aattctgctt tactttttga gtttgctgta ctaaaacggt atgcagaacc gttactttct
    19921 gaaataccta aatattgtga tgaatatacg gaagcgcatc gtttattaaa atttttaagt
    19981 tattttgttg ccattcctga tcgtgaaatt ccgcctactt ctttactgcg ggaatttgtt
    20041 ggaggaagta gttttagata ttagtaaaaa aagactgaaa aatttttcaa taaaaattaa
    20101 tattatttgg aagcaattat ttaacaagtt caaaaaatta atactatgaa aaaattttta
    20161 gttataattc aacttgtcat tttatgctta ttccctattt atagtcaaaa aaaaggatta
    20221 ataagtactg gtggtagttc tatgtctgtt gttttaaact ctgtaagctt ggcatattgt
    20281 tttggtgatg ttggtgggtc ctatcaggaa caatttttaa acggtattaa tgattgggat
    20341 atttctaata cgcgttattt agtttcttta ggactccgac agtcatttaa aaatgattta
    20401 ggttataaaa taaatgtatt gtatggatca tttgtaggag atgatgctaa ttcaagaaat
    20461 gaaatgagag attattctta taaaaataaa gtagttggct tttctttgca agctgaatac
    20521 gttattttag gtggttttga tccttataaa ttgaatttac acacgttgta tatttattta
    20581 ggtggaggta ttttaaatag cgattcaaaa ttatggagaa aaggactatc tatttctgaa
    20641 actccatcgg atagacctta cgataaagtt aaactcacag atacgacacc tattatccca
    20701 gtaggtatag gttatcaata tcatctcact tctaattttt cgataggtgc agaattagga
    20761 tggatttatt ctttttctga ttatcttgat ggtattatta ctcaatattc taaaagaaaa
    20821 gatactttgg caaatttgaa tataacgttc acttacaagt ttaaagatac tcttacatta
    20881 ccctgtcatt gtttatctta tgaaaagtaa ttttctagaa tattgaaata aattaaaaac
    20941 atttgctatc attacataat aaaaatttcc tttcacctta ccaaaaatta accatagcat
    21001 atttataact ctaatattta attatgtagc ggtttataat ttttctttat ggatagatat
    21061 tgtgtgaaaa agatgaaaaa gatgaaaaag caatttttta ttgattcact cttcttttta
    21121 tatctttgca aaatattcta ttatctacat ttttatgtat gcttccgatt ttagatgaat
    21181 atcattatta tttaaaactt gaaaaaggcc tgtcagacaa tacgattgat gcttatgaaa
    21241 gagacttgtc aaaactatca caatatttat cagaggctca tatacctttg gaacaagcta
    21301 ctaccgagca tcttcgtgat ttcctcatcg aaattcggga cttaggaatt catcctcgtt
    21361 cgcaggcacg aattatctct ggtatcactt ctttttatca tttcttgatt tataaaaata
    21421 ttattactga agatcctacc gaattactcg aaagtcttaa aattggtttg cggctgccag
    21481 aagtgctttc cgtaagcgaa atcaatgcta tggttgctgc tattgactta agta
//
LOCUS       DAOSAS010000010        21478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_1743_length_21478_cov_13.640527, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000010 DAOSAS010000000
VERSION     DAOSAS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21478
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1743_length_21478_cov_13.640527"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PLR72_01020"
     CDS             <1..1070
                     /locus_tag="PLR72_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35826.1"
                     /translation="SLKNPGMKIKILSLAILLLVAAQAPAQRAFTIEAQNYDISDNLD
                     LRAVVSIFAESKNLKDFEMRLNDERYQISNLDLNNDGWIDYLRVVETSERDLHLVVIQ
                     AVLDRDTYQDVATIVVEKRNNPQIYVRIIGNPYLYGVNYIIEPVFVREPVIFTFFWSS
                     HYRPWRSPYYWEYYPKFYHPRPPVKVNIYMGHIHRYIPRDCKFYYHTTVNIYHNDVYV
                     KLHQTVRRNDYERKHPEKSFNNRNKGIANKAEFNSARRTSSEANRRKVNQTNENYRKT
                     SSRNLQSSSSSGNDERRNQNSSRGTEINNRPQQEQNVSGSQRRSAESSASASPSQKSS
                     TRSSRGNAAEHSNNQKHSERR"
     gene            complement(1120..2223)
                     /locus_tag="PLR72_01025"
     CDS             complement(1120..2223)
                     /locus_tag="PLR72_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOK35827.1"
                     /translation="MEFESILLPGIEAKRPIVIAGPCSAETEEQVIETARELASFGIK
                     IFRAGIWKPRTKPGGFEGVGVKGLPWLKRVKTEFGMYTTVEVANAKHVHEALANEVDI
                     LWIGARTSANPFAVQEIADALEGIDIPVLIKNPINPDLDLWIGAIERIYNAGIRRIAA
                     VHRGFSSYDKKIYRNLPQWHIPMELHRQIPNLPLFCDPSHIGGKRELIGPLSQQAMDL
                     NFDGLMIESHCNPDEAWSDAAQQVTPKILDFILNSLVIRETTQTTENLTALRRQIDEL
                     DNDLLQLLAKRMRVSEEIGVYKKEHNMPIFQPERYDEMLKDRVKQAIQMGMDGDFMKN
                     VLIAIHEESIRHQMVIMEKSDATLNNSNSSTIQ"
     gene            complement(2277..3428)
                     /locus_tag="PLR72_01030"
     CDS             complement(2277..3428)
                     /locus_tag="PLR72_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOK35828.1"
                     /translation="MNIQPAKRLNNVSEYYFSAKLREIDQMNAAGKKVINLGIGSPDL
                     MPSPQTIEALCQEAHKPNVHGYQSYNGIPELRNGFAEWYKKWFHVDLNPDNEIQPLIG
                     SKEGILHITLAFVNPGDSVLVPNPGYPTYTSVSKLCEANVIPYDLNENNNWQPDFEKL
                     EKMDLTNVKLMWVNYPNMPTGANASVELFEKLVDFSKRHNIVICNDNPYSFILNDKKL
                     SILSIPGAKDICIEMNSMSKSHNMAGWRIAMLASNAQFVQWVLKVKSNVDSGMFRPMM
                     IAAATALKNSEEWHNEMNKIYARRQQLAFKIMDQLGCTYDPKQVGLFVWGKIPAHYAD
                     AGELADEILYKKNVFITPGFIFGDKGKRYIRISLCCAEPVLEEVLKRLS"
     gene            complement(3486..4319)
                     /locus_tag="PLR72_01035"
     CDS             complement(3486..4319)
                     /locus_tag="PLR72_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOK35829.1"
                     /translation="MKRIAIQGISGAFHEIAAREYFKNEEIEIVPCNTFKDVFSAIEN
                     DKTLLGIIAIENTIAGSLLQNHNLLRESECLIVGEQKLHIEHHLVALAGQQLEDLEEV
                     HSHPIALMQCEEFLESHKHLKAVESEDTALSAKEIVDYQIYKRAAICSLYAAQKYGLE
                     VIERNIETNKKNSTRFLIVAEPTLALEMTRNDKINKASLVFALPHLEGSLSQVLSILS
                     FYNMNLTKIQSLPIIGREWEYQFYIDLIFNDYSRYKQAIDAILPLIDEFQILGEYHVD
                     I"
     gene            complement(4579..7197)
                     /gene="alaS"
                     /locus_tag="PLR72_01040"
     CDS             complement(4579..7197)
                     /gene="alaS"
                     /locus_tag="PLR72_01040"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOK35830.1"
                     /translation="MTSQEIRQSFLDFFAEKDHKIVPSAPMVIKDDPTLMFTNAGMNQ
                     FKNIILGNDPIKYPRVANVQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAIKWAWEYLVKVLKIDPDRLYVTVFEGSPADGLERDEEAAAIWSKFLSPERIINGS
                     KKDNFWEMGEVGPCGPCSEIHIDIRKDEERLKTDALTLINKNHPQVIEMWNIVFMQYN
                     RKANGSLMELPAKVIDTGMGFERLCMVVQGKTSNYDTDLFQPIIKEIGQICKAEYGHD
                     EKIDVAMRVVADHIRAIAFSIADGQLPSNTKAGYVIRRILRRALRYGYTFLHQRSPFL
                     FKLVDVLCQTMGSAYPELIDQKSLIEKVIKEEEESFLRTLETGIQLLEKMIEKAINEK
                     KKEISGKDAFMLYDTFGFPIDLTELILRENNLSVNMNEFNAEMEKQKERARNAANVET
                     GDWIILREGESQFVGYDVTSCNTEILRYRKVKQKNNEFYQLILSKTPFYAEMGGQVGD
                     SGWLLCGEDKIEILDTKKENNLSVHIVKRLPANLTDRFIASVDIEKRLATSRNHSATH
                     LLHEALRQVLGTHVEQKGSYVGPEGLRFDFSHYQKLTKKEIRAVEAIANRKIRENIPL
                     NEQREIPIQKAREMGAMALFGEKYDETVRLVQFGTSLELCGGTHVKATGEIGMVRIIS
                     ETSIAAGIRRIEAITGEAVEKLIDTQQDLLNEAKELLNNSPDLITAIRKLLEENRELK
                     KLVNEYNEEKLLQLRDKLLREAEIVEGITVVCYEGNLTSDQAKALAFQIKNLQNSNLF
                     FIAGNVHEGKPSLTVLITEDLVKKGFNAIDIVRKAAKEIQGSGGGQPFFAVAGGKNPQ
                     GIHKAVALTRELIKKE"
     gene            7400..8368
                     /locus_tag="PLR72_01045"
     CDS             7400..8368
                     /locus_tag="PLR72_01045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOK35831.1"
                     /translation="MAKKKYIFNPETLNYEPVEYTFGYKIRRFLVHTFTGIMIGTLFF
                     FVLALTLQSPEEKQLNDEKNKIEAQYQILERQFNEAQVVLNDLQERDNNLYRVILQAE
                     PIPFSVRRAIAGNADYYHQLLNMTNSEIVVNTTKELNVLKKQLYLQSKSYDELILLAK
                     NKETMLQHLPAIQPVLNKDLARMASGFGWRIDPIYHTQRFHEGMDFVAPTGTEIFATG
                     DGTISRAGWEQGYGNCVKISHGFGYETLYAHMSKIKVRIGQRVKRGDVIGLVGNTGKS
                     TAPHLHYEVHYKGQIMNPQNYYFLDLSPEEYDRMVQMSNNSGQLLD"
     gene            8397..9272
                     /gene="murI"
                     /locus_tag="PLR72_01050"
     CDS             8397..9272
                     /gene="murI"
                     /locus_tag="PLR72_01050"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOK35832.1"
                     /translation="MQSNIKDCLKDLSHNFESKPGPIGIFDSGYGGLTILEKIRQCLP
                     QYDYVYLGDNARTPYGTRSFEIVYKYTLECVVQLFKMGCHLVILACNTASAKALRTIQ
                     QNDLPILDSHRRVLGIIRPTVEAIGSITNNFHVGLLATSGTVQSNSYGLEIQKLYDNI
                     VLTSEACPMWVPLIENSEYNSPGADYFINKNLNHLLQIDPEIDTIILGCTHYPILENK
                     IKSLLPNKITVVSQGKIVAESLKNYLQRHPEIETLCTKKGTFQYYTTESVEKFSMMAS
                     IFLKENIQAQHIEIG"
     gene            9479..11638
                     /locus_tag="PLR72_01055"
     CDS             9479..11638
                     /locus_tag="PLR72_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOK35833.1"
                     /translation="MKVYQPNEIKNISLLGSAGSGKTTFAEAILYEAGIIKRKGSIAN
                     QNTVSDYYPVEKEYGYSVFPTVIQTEWQHNKLNFIDCPGADDFIGGVVTSLNVTDTAL
                     ILVNSQYGVEVGTTNHFSYAEKNNKPVIFVMNHLDHEKVNYELSLESLTENFGNKVIQ
                     VQYPFNPGLEFNAIIDLIQMKMLQWKTEGGNPEILEIPAEEMEKAKQLHSQLVEAAAE
                     HDEALMEKFFEEGVLTEEEICNGLRKGVRQRGVFPVFCISALKNIGVSTLMDYLVKIA
                     PSVAESEWSETKDGKKVEPNPNAPTSLYVFKTNVEPHVGEISLFKVMSGTVHEGDDLY
                     NINRGVKEKINQLFNICGQIRNKVEEIGAGDIGATVKLKDTRTGNTLNGKGCDYDFKE
                     INYPNPKYRRAVKAMSEADEEKLNEVLTRMHEEDPTWIVEQSKELKQTILHGQGEFHL
                     RTLKWRVENNDKIEIEFKEPKIPYRETITKQARADYRHKKQSGGAGQFGEVHLIIEPY
                     SEGLPIQDVYRFNGQEYKISVRDVQEIDLEWGGKLIFVNSIVGGAIDARFMPAILKGI
                     MERMEQGPLTGSYARDVRVIVYDGKMHPVDSNEISFRLAGRNAFVRAFKEAGPKLLEP
                     IYDVEVLAPSDRLGDVMSDLQSRRAVIMGMHSEKGFEKLNAKVPLKELGNYSTTLSSL
                     TGGRATFNMKFASYELVPMEIQEKLLKEYEATRKEEE"
     gene            11843..12538
                     /locus_tag="PLR72_01060"
     CDS             11843..12538
                     /locus_tag="PLR72_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275865.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOK35834.1"
                     /translation="MKNRIVESSIIAVGIICLGLFIQNGIKIFSQKDRAVEVKGLAEM
                     EVPANKVICPILYKDLGNDLAQLYIELNKKNKAIISFLKENGIHDNEISVSAPEIVDM
                     EAERFIENKSPYRYNLTSVITITTENVDKVRALMTRQAELLKQGIAVTVNNYSVRYEY
                     TKLNEIKPQMIEEATKNARKAAEKFAKDSGSKLGKIKNATQGQFVISDRDENTPYIKN
                     IRVVSTITYYLED"
     gene            13007..14095
                     /locus_tag="PLR72_01065"
     CDS             13007..14095
                     /locus_tag="PLR72_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOK35835.1"
                     /translation="MYLESLVILNYKNIQEANLMFSPKINCFIGKNGMGKTNLLDAIY
                     FLSFCKSHSNFIDSQNIYHQADFFLLQGQYQVDENIEEIYCGLKRNQKKQFKRNKKEY
                     ERLSDHIGLLPLVMISPEDITLISGGSEERRKFIDGVISQYDKNYLHALFRYNNALRQ
                     RNALLKADSYVEDSLFEIWEEQMAQYGSYLFEQRQLFIEKMIPVFEKYYHYLSNGKES
                     IQLNYLSHHQQYDIKIRLKETREKDKILGFSTQGIHKDELEMLLDGYPIKRVGSQGQN
                     KTFLISLKLAQFNFLSHTHQQFPILLLDDIFDKLDTDRVKRILELVSQESFGQIFITD
                     TDREHLNQILSQLQQETKIFKVENGTIC"
     gene            14398..16947
                     /locus_tag="PLR72_01070"
     CDS             14398..16947
                     /locus_tag="PLR72_01070"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOK35836.1"
                     /translation="MKAKKYTSDEVMEASLKYFQGDDLAAKVWTTKYALKDSFGNFYE
                     LTPDDMHHRLAREIARIEKKYPQPLSEEELFELLRNFKYIVPQGSPMSGIGNNFQIAS
                     LSNCFVIGFDHDADSYGGIIKIDEEQVQLMKRRGGVGHDLSHIRPKGSPVKNTALTST
                     GIVPFMERYSNSTREVAQDGRRGALMLSISIKHPDAESFIDAKMEANKITGANISVKI
                     DDEFMKAALEGKPYIQQFPIDEPEPRVKKEIDAATLWKKIIHNAWQSAEPGVLFWDTI
                     IRESIPDCYADLGFKTISTNPCGEIPLCAYDSCRLLAINLYSYVDRPFTSEASFNFEL
                     FKKHVALAQKIMDDIIDLEIENIDQILSKIKSDPEDEEIKRTEYMLWEKIRAKTLQGR
                     RTGVGTTAEGDMLAALGYRYGTTEAIDFSEHVHKILALSAYRSSVTLAQERGAFSIYD
                     SKREENNPFIHRIRQADPDLYNDMVKYGRRNIACLTIAPTGTTSIMTQTTSGIEPVFM
                     PVYTRRRKVNPNDKNVQIDFVDDNGDSWEEYTVFHHKFVTWMEANNYPTTKKYSKSEI
                     DDLVAKSPYYKATANDINWLKKVQMQGRIQKWVDHSISVTINLPAETTEELVADLYEE
                     AWRCGCKGVTVYREGSRSGVLVSMDEAQKEKQEEKKVCFPVTEGPLVRPKELECDVVR
                     FQNAKDKWIAFVGLKDGRPYEIFTGLADDEEGISLPKTVTHGKIIKTVDENGNKRYDF
                     QFTNKRGYKTTVEGLSEKFDKEFWNYAKLISGVLRYGMPIDQVVKLVSGLQLDSDSIN
                     TWKVGVERALKKYIPDGTQADGQTCPSCKQKTLIYQEGCLTCKNCGYSRCG"
     gene            17930..18379
                     /locus_tag="PLR72_01075"
     CDS             17930..18379
                     /locus_tag="PLR72_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35837.1"
                     /translation="MSENEAKKIYYEPMHTCEHILNQTMIRMFGCERSKNAHIEKKKS
                     KCDYFLPQPPTEAQIFELTRRVNEVINQNLPIRVEYMTHEEAAKIVDLSKLPTATPDL
                     LRIVHVGNYDACACIGQHVQNTSEIGRFEIVSHNYENGCWRVRFRLV"
     gene            18410..19129
                     /gene="mtgA"
                     /locus_tag="PLR72_01080"
     CDS             18410..19129
                     /gene="mtgA"
                     /locus_tag="PLR72_01080"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008017352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOK35838.1"
                     /translation="MKRFGKFLKNFFVLFVIISVGWVIFYRFVPVFFTPLMLIRSVEA
                     ISDGEAPKINKKWVSIEKISPNMIQAVAAAEDDQFMEHHGFSFEAIHKAIQHNKRSRR
                     IRGGSTISQQTAKNVFLWPHRSYLRKGLEAYFTVLIELFWTKERIMEVYLNVIETGDG
                     IYGVEAASLEYFGKHASKLTKPEAALIAACLPNPRRFNPAHPSPYILRRQAAIMRMME
                     KISPVEFHKTSVFKKTKHKHR"
     gene            19137..19802
                     /gene="lipB"
                     /locus_tag="PLR72_01085"
     CDS             19137..19802
                     /gene="lipB"
                     /locus_tag="PLR72_01085"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOK35839.1"
                     /translation="MNKQIQYLDWGIIEYEEALRKQEILFSDTIERKKSGLSTSNYFI
                     FCEHPSVFTLGKNGQANNLLISANELRKINATFSLTNRGGDITYHGPGQIVGYTIFDL
                     ENFQLGLKQFVEKIEETIIQLLKLYQISATRLKGATGVWLDAATERVRKICAIGLRSS
                     HFITMHGFALNVNTNLDYFNYIHPCGFVDKGVTSMEKELKQKVDIVQVKENLKQCLEE
                     QFT"
     gene            20067..21245
                     /locus_tag="PLR72_01090"
     CDS             20067..21245
                     /locus_tag="PLR72_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOK35840.1"
                     /translation="MWKIIISVIPMFVCLFWCIVLFIEHHTQNKAKTFLSFFMFVAFL
                     LYLGHAAYFNKEYKFYGFWDSIYLFCSLAVYPLYFIYLKIITKKTKLNFKDFFVLLPA
                     FTIGIFSFILYFLMSPEELTVYTQQLIYHNFNNIEWTSILHLQQIKLYLFTIIFVIQI
                     IPILIYGSKYIKEYNQAIMNYYADTEKKTLDHFNFLLIVFILTSLASSTVDLIGRSYF
                     TQSNIHLLIPAIIFGFLLFMVGYLGYKQDFSIENFLKDIKNNEALARTIKISINDTEL
                     YGKNLFENLKKLLEENEIYKNKDLCITDIAIMLNTNRTYISKIINEKFKTNFSTMINH
                     YRIEHAKKILKDSKYRQLSINEIGELSGFSSESSFYRIFKNHENISPGNYRKKHLINY
                     "
BASE COUNT         7330 a         3667 c         3442 g         7039 t
ORIGIN      
        1 gatccttaaa aaatccaggt atgaaaataa aaatattgag tttagcaatt ttacttttag
       61 ttgctgctca ggcgcccgca caaagagctt ttacaataga agctcaaaat tacgatataa
      121 gcgataatct cgatttgaga gcagttgttt ctatttttgc ggaatcaaaa aatttaaaag
      181 attttgaaat gagattgaat gatgaacgtt atcaaatttc aaatcttgat ttgaataatg
      241 atggctggat tgattattta cgtgtggtag aaacttctga aagagatttg catcttgttg
      301 taattcaggc agtattggat agagacacat atcaggatgt ggcaaccata gtagttgaga
      361 agcgtaacaa tcctcaaatt tatgttcgaa ttattggaaa tccgtattta tatggagtga
      421 attatattat tgaacctgtt tttgtgcgag agccagtaat ttttacattc ttctggagta
      481 gccattatcg tccttggcgg tcaccttact attgggaata ttatccaaaa ttttatcacc
      541 ctcgtccacc tgtgaaagta aatatttaca tgggacatat tcacaggtat ataccacgtg
      601 actgtaaatt ttattatcac acaacggtta atatctatca caacgatgtt tatgtaaaac
      661 ttcatcaaac tgttcggcga aatgattatg agcgaaaaca tccagaaaaa agttttaaca
      721 atcgcaacaa aggtatagcc aacaaagctg aatttaattc tgctcgtcga acatcatcag
      781 aagcaaatcg tcgaaaggta aatcagacta atgaaaatta cagaaaaacc tcttctcgta
      841 atttacagtc atcaagtagc agtggaaatg atgaaagaag aaatcaaaat tcttctcggg
      901 gaacagaaat aaataatcgt cctcaacaag aacaaaatgt ttctggttcg cagagacgtt
      961 ctgctgaatc ttcagcttca gcttcacctt cacaaaagtc ttcaactcga tcgtcacgtg
     1021 gaaatgcagc agaacattct aataatcaaa aacattctga aagaagataa aaacttttca
     1081 ataaattttt aggatcgatc tttacttttc tttaataatt cattggattg ttgaagaatt
     1141 cgaattattt aaagtggcat cagatttttc cataattacc atttggtgac gaatcgattc
     1201 ttcatgaata gcaatcaata catttttcat aaaatcgcca tccatcccca tttgaatagc
     1261 ctgtttcact ctgtccttta acatttcatc ataacgttca ggctggaaaa taggcatatt
     1321 gtgttctttt ttatacactc caatttcttc tgaaactctc attcttttag ccaataattg
     1381 aagtaaatca ttatcaagct catctatttg acgccgaaga gcagttaaat tttcagtggt
     1441 ttgggtggtt tcacgaatga ccaatgaatt taaaataaag tctaaaattt tgggcgtaac
     1501 ttgttgagcc gcatcactcc aggcttcatc aggattacaa tgcgattcaa tcattagccc
     1561 atcgaaattc aaatccattg cttgctgact caagggacca attagttcac gtttcccacc
     1621 aatatggctg ggatcacaaa acaagggcaa attaggaatt tgtctatgta attccattgg
     1681 aatgtgccac tgaggcaagt ttcgataaat ttttttatca taagaactaa atccacgatg
     1741 gacagcagcg attcgcctaa ttcctgcatt gtaaatccgt tcaatagctc caatccacaa
     1801 atctaaatca gggttaattg gatttttaat tagtacagga atatcaattc cttctaaagc
     1861 atcagcaatt tcttgaacag caaatggatt tgccgaagta cgtgcaccaa tccataaaat
     1921 atctacctca ttagccaaag cttcatgaac atgtttagca tttgccactt ctacagtggt
     1981 atacataccg aactctgttt ttacacgctt aagccatggt aatcctttca ctcctacacc
     2041 ttcaaagcca ccaggtttgg tgcggggttt ccaaattcca gctcggaata tttttattcc
     2101 aaaagaagcc aattcacgtg ctgtttctat tacctgctct tcagtttcag cactacaagg
     2161 acctgcaata acaattggtc gtttggcttc tatacccggt aataaaattg attcaaattc
     2221 catagtaaat attattttaa caatttatcc aactattaca aaattttatt ttttatctaa
     2281 ctcaatcttt ttaaaacttc ctctaaaaca ggttctgcac aacacaaaga tatccgaata
     2341 tatcgtttcc ctttatcacc gaaaataaaa ccaggagtaa taaatacatt ctttttatat
     2401 aatatttcgt cagctaattc gccagcatcg gcatagtgag caggaatttt cccccataca
     2461 aataatccaa cctgcttggg atcatacgtg caacctaatt ggtccataat tttaaaagca
     2521 agttgttgac gtcgggcata aattttattc atttcgttgt gccattcttc tgaatttttt
     2581 aaagcagtag ctgctgctat catcatggga cgaaacattc ctgaatctac attcgatttt
     2641 actttcagca cccattgcac aaattgagca ttggaagcca acatagcaat acgccatccg
     2701 gccatattat gggatttact catcgaattc atttcaatac aaatatcttt tgctcctgga
     2761 atagaaagaa tgctcagttt tttatcattt aaaataaagc tataaggatt atcattacaa
     2821 attacaatgt tatgcctttt gctaaaatca accaattttt caaataattc cacactggca
     2881 ttggcgcctg tcggcatgtt tggataattg acccacatca actttacatt ggttaaatcc
     2941 attttctcaa gtttttcaaa atcaggttgc caattattat tttcattcaa atcataagga
     3001 attacatttg cttcacataa cttgctcaca gaagtataag taggataacc aggattgggc
     3061 accagaacgc tatcgccagg atttacaaaa gccaatgtga tgtgtaaaat tccttctttc
     3121 gaaccaatta aaggttgaat ttcattatca ggattcaaat ccacgtgaaa ccatttttta
     3181 taccattctg caaatccatt tcgtaattca ggaatcccat tataactctg gtaaccatga
     3241 acgtttggct tatgagcttc ttgacaaaga gcttcaatag tttgtggaga aggcatcaaa
     3301 tcaggacttc caatgcctaa attaattacc tttttccctg cagcattcat ttgatctatt
     3361 tctcttaatt tggccgaaaa ataatattct gaaacattat ttagacgttt tgctggctgt
     3421 atattcattt gtactatttt aaaaaattac tgtaattata aatgatctaa attggtttat
     3481 aaattttaaa tatctacgtg gtactctccc aaaatctgaa attcatcaat caaaggaaga
     3541 atagcatcta ttgcctgttt gtatcgagaa taatcattaa aaatgagatc aatgtaaaat
     3601 tgatattccc attctctgcc tataataggc aacgattgaa ttttcgtgag attcatgtta
     3661 taaaatgata aaatggaaag tacctgtgaa agacttcctt ctaaatgagg caatgcaaat
     3721 accagagaag ctttattaat tttatcattt ctggtcattt ccaaagcaag tgtcggctcg
     3781 gcaacaatta aaaagcgggt ggaatttttt ttattggttt ctatgtttct ttctataact
     3841 tcaagcccat atttttgagc tgcataaaga ctgcaaatag ctgcccgttt ataaatttga
     3901 taatcaacaa tttcttttgc agataaagca gtatcttcac tttccactgc cttcaaatgc
     3961 ttatgggatt ctaaaaattc ttcacattgc atcaaagcaa tcggatgaga atgcacttct
     4021 tctaaatctt ccaattgctg cccggctaat gctactaaat gatgttcaat atgtaatttt
     4081 tgttctccca caataagaca ttcactttcg cgtaataaat tatgattttg caataaactt
     4141 cctgcaatag tattttcaat ggctataatt cccaaaagtg ttttatcatt ttctatagca
     4201 ctgaaaacat ccttaaaagt attacaaggt acaatttcaa tttcttcgtt tttaaaatac
     4261 tctcttgcag caatttcatg aaatgctcca ctaatgccct gaatagctat tcttttcata
     4321 ttcaaatgat taaaattata aataaaaaat cctgctcagc atgttgagca ggactctcta
     4381 tatgattaaa ctaaaaactt tcattcgtaa ttttacataa taattatcct gctcttactt
     4441 tttaaagtaa aagtaaaaat aaaaatatgt aaaataacga tgattcatta tctgttgttt
     4501 gttatcgcat ttgatttggc aaaaataaaa aaaaattaaa gcaaaaacaa ataaatcgta
     4561 aaaaaatttc aaaaaatttt attccttctt tattaactct cttgtcaaag caactgcttt
     4621 atggattccc tgcggatttt tacctcctgc aacggcaaaa aaaggttgtc cgccaccact
     4681 accttgaatt tcttttgctg ccttacgaac aatatcgatg gcattaaacc ctttttttac
     4741 taaatcttcg gtaatcaata cagttaaaga aggtttccct tcatgtacat ttcctgctat
     4801 aaaaaataaa ttagaattct gaagattctt aatttgaaaa gccaatgctt ttgcttgatc
     4861 tgatgttaaa tttccttcat aacaaactac tgtaataccc tcaacaattt cagcttccct
     4921 taataattta tcacgaagtt gcaaaagttt ttcttcattg tattcattaa ccagtttttt
     4981 taattctcta ttttcttcta acaatttacg aatagcagtg atcaaatcag gagaattatt
     5041 taataattct tttgcttcat tcaaaagatc ttgttgagtg tcaataagct tttctacagc
     5101 ctcacctgta attgcctcga tacgacgaat tcctgcagct atggaagttt cggaaattat
     5161 tctaaccata ccaatttccc cagtagcctt tacatgagtt cctccacaaa gttccagcga
     5221 agtaccaaat tgaacaaggc gtactgtttc atcatatttt tctccaaaaa gtgccattgc
     5281 tcccatttca cgagcttttt gaataggaat ctcacgttgt tcattcaaag gaatattttc
     5341 tctaatttta cgattagcaa ttgcctctac tgctcttatc tctttttttg tcaatttctg
     5401 ataatgagaa aaatcaaaac gcaatccttc aggtcctaca taagagcctt tttgctccac
     5461 atgtgttcct aaaacttgtc gcaaagcttc atgcagcaag tgagtagctg agtgatttcg
     5521 tgaagttgcc aaccgttttt ctatatcgac agaagcaata aacctatccg tcaaatttgc
     5581 aggaagtcgt tttacaatat gaacagaaag attattttct ttttttgtat cgagaatttc
     5641 aattttgtct tcaccacaaa gcaaccaacc tgaatcgcca acttgccctc ccatttcagc
     5701 atagaaagga gttttggaaa gaattaattg ataaaactca ttattttttt gctttacttt
     5761 tcgatagcgt aaaatttctg tattacaaga agttacgtca tatcccacaa attgactttc
     5821 cccttcacga agaataatcc aatctcctgt ttctacatta gcggcattcc gagcgcgttc
     5881 tttttgtttt tccatttcag cattaaattc attcatatta acgctaagat tattttcacg
     5941 taaaattaat tctgttaaat caatgggaaa tccaaaagta tcatatagca taaaagcgtc
     6001 cttgccagag atttctttct ttttttcatt tatagctttt tctatcattt tttccagcaa
     6061 ctgaattcct gtctcaagcg tccgtaaaaa ggattcctct tcttctttga taactttttc
     6121 aatcaaagat ttctgatcta tcaattctgg ataagctgag cccattgttt ggcaaagaac
     6181 atccaccagc ttgaataaaa aaggagaacg ttgatgtagg aaagtataac catatcgtaa
     6241 agcgcgacgt aaaatccgcc gaattacata acctgctttg gtgttagaag gaagttgtcc
     6301 atctgcaata gaaaaagcta tggctctgat atgatcagca actactcgca ttgctacatc
     6361 aattttttca tcatgcccat attcagcttt gcaaatttgt ccaatttctt taataatagg
     6421 ttggaacaaa tctgtatcat aattggaagt ttttccctgt actaccatgc acaaacgctc
     6481 aaaacccatt cctgtatcaa ttacttttgc aggcaattcc attaatgaac cattggcttt
     6541 tcgattgtac tgcataaaaa caatattcca catttcaatt acctgtggat gatttttatt
     6601 gattaaagtc aaggcatcag ttttcaatcg ttcttcatct ttacgaatat caatatgaat
     6661 ttcagaacaa ggtccacaag gtcccacttc gcccatttcc caaaaattat cctttttact
     6721 tccattaata attctttcag gagaaagaaa ctttgaccaa atggcagcag cttcttcatc
     6781 ccgttccaaa ccatctgctg gtgaaccttc aaaaacagta acatacaatc ggtcaggatc
     6841 aattttaagt acttttacca aatattccca tgcccattta atagcttctt tcttaaaata
     6901 atctccaaaa gaccagttcc caagcatttc aaacatggta tgatgataag tatcatgccc
     6961 tacttcttcc aaatcattgt gcttaccgct cacacgtaaa catttttgta cattggctac
     7021 acgtggatat tttataggat cattacctaa aataatattt ttaaattggt tcatcccagc
     7081 attggtaaac attaaagtag gatcatcttt tattaccata ggagcagatg ggacaatttt
     7141 atgatctttc tcagcaaaaa aatccaaaaa agattgacga atttcttgtg aagtcattag
     7201 atatatcgtt gtaattcaat tataacttat tcaaaaatac aattttattt ttaaagttaa
     7261 tattcttaaa tgaagtctgc aaaattagtt taaatttatt atttttgtat cataagctcg
     7321 ttttaaattt atttttaatg gcaaatttat attttctctc attaatattt cactaattta
     7381 attaagaaag gatattgcta tggcaaagaa aaaatatatt tttaatcctg aaacattaaa
     7441 ttatgagcct gtggaatata cttttggtta taaaatcaga agatttttag ttcatacttt
     7501 cacaggaatt atgataggta ctttattctt ctttgtactt gctttaacat tacaatctcc
     7561 tgaagaaaaa caattgaatg atgaaaaaaa taaaatagaa gcacaatatc aaattctgga
     7621 acgtcagttt aatgaagcac aagttgtgct taatgatttg caagaaagag ataataattt
     7681 atatcgagtt attcttcagg cagaacctat tcctttttct gtccgtcgtg ccattgcagg
     7741 aaatgctgat tattaccatc aacttttaaa tatgaccaat tcggagatcg ttgtaaatac
     7801 tacaaaagaa ttgaatgtct taaaaaaaca actttatctc caatcaaaat cttatgatga
     7861 attgatattg ttagcaaaaa ataaagaaac aatgcttcaa caccttcctg ccattcagcc
     7921 cgtgttgaac aaagatctcg cacgtatggc atctggattt gggtggagaa ttgatcccat
     7981 ctaccatacg caacgatttc atgaaggaat ggattttgta gcaccaacag ggacagagat
     8041 ttttgcaaca ggagacggaa ctattagtag agctggttgg gaacaaggtt atggaaattg
     8101 tgtgaaaatt agtcatggtt tcggttatga aactttatat gcccatatga gtaagataaa
     8161 agtacgaatt ggacaacgtg ttaaacgtgg tgatgtcatt gggttggtag ggaacacagg
     8221 taaatcaact gccccacatt tacattacga agttcactat aaaggacaaa tcatgaaccc
     8281 gcaaaactat tatttcctcg atttatcgcc tgaagaatat gatcgtatgg ttcaaatgag
     8341 taacaattca ggtcaattgt tagattaaaa ctactgaatt aaaattcatt tcaatcatgc
     8401 aaagcaacat aaaagattgt ctaaaagatt tatcacataa ttttgaaagt aaaccaggtc
     8461 ccattggaat ttttgattca ggatacggag gattgactat tttagagaaa attcgtcagt
     8521 gtttacctca atatgattat gtttatttag gcgacaacgc acgtacacct tatggtacac
     8581 gatcatttga aatagtgtat aaatatacat tggaatgtgt tgttcagctt tttaaaatgg
     8641 gttgtcattt ggtaatttta gcttgtaata cagcttcagc aaaagcttta agaaccattc
     8701 aacaaaacga ccttcctatt ttggactctc atcggcgtgt tttaggtatt attcgtccaa
     8761 cagtagaagc cattggttct attacaaata attttcacgt gggtctactt gccacctctg
     8821 gaactgttca atcaaactcc tatgggcttg aaatacaaaa attatatgat aacattgtct
     8881 taacttctga agcatgtcct atgtgggttc cacttatcga aaattctgaa tataattctc
     8941 caggagctga ttattttatc aataaaaatt tgaatcatct cttgcaaatt gatcctgaaa
     9001 ttgatacaat tatattaggc tgcacgcatt atcctattct ggaaaacaaa ataaaatctt
     9061 tactacctaa taaaattact gttgtttcgc aaggaaaaat tgttgctgaa agtttaaaaa
     9121 attatctcca gcgtcatcca gaaatagaaa cgctctgtac aaaaaaagga acttttcaat
     9181 attatactac tgaatcagta gaaaagtttt ctatgatggc ttctattttt ttaaaagaaa
     9241 atattcaagc tcaacatata gaaattggtt aattctattg aaaattacgt ttcattggaa
     9301 aaaacaccaa tattaataac ccccaaaaaa gggtatcaat ttatgtttta aaacattatt
     9361 ttaaaaacat aaaaaaatct ccttttataa taggagagtt ttttataact tgccatccaa
     9421 tttttgcatt ataaaaaaca ctacgtaatt attataatat tctaaaaatg aatgagatat
     9481 gaaagtttat caaccaaatg aaattaaaaa catctcttta ttgggaagtg caggttctgg
     9541 aaaaacaact tttgctgaag ctattctcta tgaagcaggg ataattaaac ggaagggttc
     9601 cattgccaac caaaatacag tttcagatta ttatcctgtt gaaaaagaat atggttattc
     9661 agtttttcca acggttattc aaacagaatg gcaacataat aaattgaatt ttattgattg
     9721 tccaggtgcg gatgatttta ttggaggtgt ggtaacttca ttgaatgtca ctgatacagc
     9781 tttgattctt gtcaattctc aatatggcgt tgaagtagga acaacgaacc acttttcata
     9841 tgctgaaaaa aataataagc ctgtaatttt tgtaatgaat catttagatc atgaaaaagt
     9901 taattacgaa ctctcattag aatcactaac agaaaatttt ggcaacaaag tcattcaagt
     9961 acaatatccc ttcaatccag gattagaatt caatgccatt attgatttaa ttcaaatgaa
    10021 aatgctgcaa tggaaaaccg aaggtggcaa tcctgaaatt cttgaaattc ctgctgaaga
    10081 aatggaaaaa gccaagcagc ttcatagcca gttagtagaa gcagccgccg agcatgatga
    10141 agctttaatg gaaaaatttt tcgaagaagg cgttcttaca gaagaagaaa tctgcaatgg
    10201 tcttcgcaag ggggtacgtc aaagaggagt tttccctgtt ttttgtatct cagcattaaa
    10261 aaatattgga gtaagcactt taatggatta tttggtaaaa attgctccca gtgttgccga
    10321 aagtgaatgg tctgagacca aagatggaaa aaaagtagaa cccaatccca acgcccctac
    10381 aagcctttac gtatttaaaa ccaatgtaga acctcatgtt ggagaaattt ccttatttaa
    10441 agtgatgagc gggacggtac atgaaggtga cgatttatat aacattaata gaggagtaaa
    10501 agaaaaaatt aatcaacttt ttaatatttg tggacaaatt cgcaacaaag tagaagaaat
    10561 tggtgcaggc gatataggtg ctaccgttaa attgaaagac acacgtaccg gtaacacctt
    10621 aaatggaaaa ggatgcgatt atgattttaa agaaataaat tatcctaatc caaagtaccg
    10681 tcgagcagta aaagcaatgt cggaagctga tgaagaaaaa ttaaatgaag ttttgacccg
    10741 catgcacgaa gaagatccta cctggattgt agaacagtca aaagaactta agcaaactat
    10801 tttgcatggt caaggtgaat tccatcttcg tactttaaag tggagagttg aaaataatga
    10861 taaaattgaa atagaattta aagaacctaa aataccttat cgcgagacca tcaccaaaca
    10921 agcacgagct gattatcgcc acaaaaaaca atcgggtggt gcaggtcaat ttggggaagt
    10981 acatttaatt attgaacctt attctgaagg cttacctatc caagatgttt atcgtttcaa
    11041 tggacaagaa tataaaatta gtgttcgtga tgttcaagaa attgatctgg aatggggagg
    11101 aaaattgatt tttgtcaaca gtattgtcgg tggagccatt gatgcacgat ttatgccggc
    11161 tatattaaaa ggaatcatgg agcgtatgga acaaggaccc ttgacgggct cgtatgctcg
    11221 agatgtaaga gtgattgttt acgacggtaa aatgcatcct gtagattcca atgaaatttc
    11281 attccgtttg gcaggaagaa atgcttttgt acgagccttt aaagaagcag gtcctaaact
    11341 attagaaccc atttacgatg tagaagtact tgcaccgtca gatagattgg gcgacgtcat
    11401 gagtgatttg caaagccgac gggcagttat catgggaatg catagcgaaa aaggttttga
    11461 aaaattaaat gcaaaagtac ctctgaagga attaggaaat tattctacca cacttagttc
    11521 actcactgga ggaagagcaa ccttcaacat gaaatttgcc agttatgaac tcgtcccaat
    11581 ggaaattcag gaaaaattac tgaaggaata cgaagcaact cgcaaagaag aagaataaat
    11641 gtggtaaatc caaaatatta attcaaatga gttattaatt aatcgttaat taatttgatg
    11701 tattctatta attatcaata aaataacaat aaaatatagg tgaacagtga atgttaaact
    11761 ttttaaggat taataacgta aaaacatctt gaatttatcc tttaaaacag taatactctt
    11821 tcacaaatga aaaaccaatt ccatgaaaaa tagaatcgtt gaatcatcta ttattgctgt
    11881 aggaattatt tgcttgggac tattcattca aaatggcatc aaaatttttt cccaaaaaga
    11941 ccgtgcggta gaagttaaag ggcttgctga aatggaagtc ccagccaata aagtaatttg
    12001 ccccattcta tacaaagatt tgggaaatga ccttgctcag ctttatattg aactgaataa
    12061 aaagaataaa gccattattt catttttgaa agaaaatggt atccatgaca atgaaatatc
    12121 cgtttctgct cctgaaattg tggatatgga agccgaaaga tttatcgaaa ataaatcacc
    12181 ttatcgttat aatctgacat ctgttattac catcacaacc gaaaatgttg ataaagtacg
    12241 tgctttgatg actcgccaag ctgaactcct taagcaagga attgctgtta cggtgaacaa
    12301 ttacagcgtt cgctatgaat atactaaatt gaatgaaatt aagccacaaa tgattgaaga
    12361 agcgacaaag aatgcacgga aagcggctga aaaatttgcc aaagattcgg gcagcaaatt
    12421 aggaaaaatc aaaaacgcta cccaaggaca gtttgttatt tccgacagag atgaaaatac
    12481 accttacatc aaaaatattc gcgtagtttc aaccattact tattacctgg aagattgaaa
    12541 tcagaataca gcaattcttc tccccttcat tttcagaatt ttacctatgg gcttgttttg
    12601 aaaaacattt tttgaaacta ttgtagcttc gatatcaagc ttcatcataa tactaaacca
    12661 atatcaagat caacttttat tgatcatctt tttttcttta tatgttaatc aagataaatg
    12721 gattgtttta atggaggtaa cgtcccctta aaaaagaaga atttgttgca attccatttc
    12781 tacgaaagag tatcaactat tagcttacag agtagttaaa aaacattttt caaggcggat
    12841 tttaatctta ttagtaggac aaatttgttt ttatttgaat atctgcattt tagtactacc
    12901 agtttgatgt taataaatta aaatccgaat cattgttttt ttcttccctc tgtgaaaatc
    12961 gaaaattatc gtgtaaattt gtataaaata attttttaga cttcgcatgt atctggaatc
    13021 actggttata ttgaattaca aaaacataca ggaagccaac ttaatgtttt ctcccaaaat
    13081 aaattgtttt atcggaaaaa atggaatggg aaaaaccaat ttgctggatg ccatttactt
    13141 tttatcgttt tgtaaaagtc attctaattt cattgattct cagaatattt atcatcaggc
    13201 agactttttt ttgttgcaag gacagtatca agtggacgaa aacatcgaag agatttattg
    13261 cggactcaaa agaaaccaaa agaaacaatt taagcgaaat aaaaaagaat atgaacgatt
    13321 gtcagatcac atcggcttat tgccgctggt aatgatttct cctgaagata taacgctcat
    13381 cagcggtgga agcgaagaac gacgaaaatt tatcgatggc gtcatttctc aatatgataa
    13441 aaattatctt catgctctct tcagatataa caacgcattg cgtcaacgaa atgctttgct
    13501 gaaagctgat tcgtatgtag aagattcgtt atttgaaatc tgggaagagc aaatggcaca
    13561 atatggaagc tacctttttg aacaacgtca gctttttatt gaaaaaatga ttcctgtttt
    13621 tgaaaaatac tatcattatc tttcaaacgg aaaagaatct attcaactta attatctttc
    13681 gcatcatcag caatacgaca taaaaattcg gctgaaagaa acccgcgaaa aagataaaat
    13741 acttggattc tctacgcagg ggattcataa agatgagctg gaaatgctac tggacggtta
    13801 tcccattaaa cgagtaggtt cacaagggca aaataaaact tttctcattt ctttaaaatt
    13861 agctcaattc aattttctgt cacatactca tcagcaattt cctattctct tattggatga
    13921 tatttttgat aaactggata cagaccgcgt taaacgaatc ctggaacttg tttcgcaaga
    13981 atcattcgga caaattttta ttacggacac agatcgtgaa cacctcaatc aaattctttc
    14041 ccaacttcag caagaaacaa aaatttttaa agtagaaaat ggaacaattt gttgatcaaa
    14101 ttactcaatg attttttctt ttcagcaagc tatctaatct gataaattga aaaaaaattc
    14161 attcctcatt ttttaaaaaa ttccatttta ggaaatattt taactatctt aaattaatat
    14221 ttcattgtaa atcaacaata taatttttta aatagataat taaaaatatt ttgaaaaaat
    14281 aatgttataa aaattgtaca atttaaaaat aatttataac attgcaaata gataatttta
    14341 ttgtcatttt tgaatataaa cccttattgc ttaatctaat tattaaaaca tattatcgtg
    14401 aaagcaaaaa aatatacttc agacgaagta atggaagcga gtttaaaata ctttcaagga
    14461 gatgatctgg ctgctaaagt ctggaccaca aaatatgcct tgaaagattc ctttggaaac
    14521 ttttacgaat tgacacctga tgatatgcat catcggctgg ctcgcgaaat tgcccggata
    14581 gaaaaaaagt atcctcaacc actttccgaa gaagaactgt tcgaattatt acgcaatttc
    14641 aagtatattg ttcctcaagg aagtcctatg agtggcattg ggaacaattt tcaaatcgct
    14701 tctctgtcca attgttttgt aattggtttc gatcatgatg cagattcgta tggtggcatc
    14761 attaaaattg atgaagaaca ggtacaattg atgaaacgtc gtggtggcgt gggacacgat
    14821 ttatctcata tccgtcccaa aggttcacct gtaaaaaata cagctttaac ttctacaggc
    14881 attgttcctt ttatggaacg ttattccaat tctactcgcg aagttgctca agatggtcgt
    14941 cgtggtgctt taatgctttc tatttctatt aaacatcctg atgcagaatc atttatcgat
    15001 gccaaaatgg aagccaataa aatcacggga gccaacattt ctgttaaaat agacgatgaa
    15061 tttatgaagg cagctttgga aggaaaacct tatattcaac aatttcccat tgatgaacca
    15121 gaaccacgcg taaagaaaga aatcgatgca gctactttat ggaaaaaaat cattcataat
    15181 gcctggcaat ctgctgaacc gggcgtgtta ttctgggata ccattattcg agaatctatt
    15241 cctgactgtt atgctgattt gggattcaag accatttcta ccaatccatg cggggaaata
    15301 cccttatgtg cttatgatag ttgccgatta ttagccatta atttgtattc ttatgttgat
    15361 cgacccttta cttctgaagc cagctttaat tttgagcttt tcaaaaaaca tgttgctctg
    15421 gctcaaaaaa tcatggacga tattattgat ctcgaaatag aaaatatcga tcaaattctg
    15481 tctaaaatca aatcagatcc tgaagatgaa gaaataaaaa ggacagaata catgttatgg
    15541 gaaaaaatcc gtgctaaaac tttgcaagga agacgaactg gcgtaggaac tactgctgaa
    15601 ggtgatatgc ttgctgcact gggttatcgt tacggaacaa cagaagccat tgatttttca
    15661 gaacacgttc ataaaatact ggctttaagt gcttatcgtt cttcggtaac gctggctcag
    15721 gaaagaggag cttttagtat ttatgattca aaaagagagg aaaacaatcc ttttattcat
    15781 cgcattcgtc aagccgatcc cgatctgtac aatgacatgg taaaatatgg tcgtcggaat
    15841 attgcttgtt taaccatagc tccaactgga actaccagca ttatgacgca aacgacatcc
    15901 ggcattgaac ctgtatttat gcctgtttat actcgccgta gaaaagtaaa tccgaatgat
    15961 aaaaatgtgc aaattgattt tgtagatgat aatggcgatt cgtgggaaga atacactgtt
    16021 tttcatcaca aatttgtaac ttggatggaa gccaataact atccaaccac aaaaaaatat
    16081 tctaaaagtg aaattgatga tttagttgcc aaatcacctt attataaagc cacagccaat
    16141 gatattaact ggcttaaaaa agtacaaatg caaggcagaa ttcaaaaatg ggtggatcat
    16201 tccattagtg ttaccattaa tttacctgct gaaactaccg aagaactggt agctgattta
    16261 tatgaagaag cctggcgatg tggctgcaaa ggagtaacgg tttatcgtga aggctcgcgt
    16321 tcaggcgttc tcgtttcgat ggatgaagcc caaaaagaaa aacaagagga gaaaaaagtt
    16381 tgtttccctg tgacggaagg tccgttggtt cgtccaaaag agttagaatg cgatgtagtt
    16441 cgttttcaaa atgcaaaaga taaatggatc gcttttgtag gattaaaaga tgggcgtcct
    16501 tacgaaattt tcaccggttt agctgatgac gaagaaggaa tttcacttcc aaaaaccgtt
    16561 actcacggta aaatcattaa aacagttgac gaaaatggta acaaacgata tgattttcaa
    16621 tttaccaata aacgaggtta caaaaccaca gttgaaggat tgtcggagaa atttgacaaa
    16681 gaattctgga attatgcaaa attaatttca ggcgtgttgc gttatggaat gcctatcgat
    16741 caggtagtca aactcgtttc aggattgcaa ctggatagtg attccattaa cacatggaaa
    16801 gttggtgtag aaagagcttt gaaaaaatac attcctgatg gcactcaggc agatggacaa
    16861 acttgccctt cgtgtaaaca gaaaacactc atttatcagg aaggctgctt gacatgtaaa
    16921 aattgtggct attcccgatg tggttagatt tcctgacttc gacaattcgt caccctaaaa
    16981 atttttcatc gaaaagaggt gcaattaatt tgtgcttttg agttatcaac attgttctga
    17041 tcattgtttt catcactttt gggtagttta attctcagag tagtaattgt tttgactatg
    17101 ttcaaaactt tatccacact caaattaatg ccgtttactc ttaatcttct ttccagttct
    17161 ttgtatactt tgtaagctac aaaaaaaatg tggtattgtt atataacctt tccaactgtc
    17221 ccatttttta tcctcctcta ttaatttgcc cttttaccat aaggggtaat atcataaaac
    17281 atcaagccaa tattaccacc caaaatcttt ttggtatgtt ctacattgat ttgttgtatc
    17341 ttctcttgtt gtgtattgta tacaatttgc ccaaataacg gtaaatcttg ttaactgcac
    17401 atcttcatca aaataggatt gtaaataatc tactgtagtc gctttactca tcggttggct
    17461 cagacgagtt acaactaagt gtcggagaat atcatcctct attgtatcaa atctgacagc
    17521 ttgatatacc caatccacta tcaattgggt ataccgttga gcaggatgtt ttctatattc
    17581 gtgagaagat attctgtaac ctgtttttct tcggcttcct gatgagattg ctcaaaaata
    17641 tcctgttcac ccaaatgagc agaaatccat tttttgcctt gaaaaataag tttcctttat
    17701 cttttatttc ccctgaaaaa gttgcattaa ttacttgaat ttacgtcata tgccatcagg
    17761 taaacaaaaa tctcctgata aaatgagaca aaaaaaccat ttcaaattca taaaaaatga
    17821 tggtattttt tgaatttttt caatacataa ttttctgtat ttttttaaaa acaagcgaat
    17881 cctcatttct caggatttat ttatctttgc attttaaaaa tattgaaata tgtctgaaaa
    17941 tgaagctaaa aaaatttatt atgagcctat gcatacatgt gagcatattt taaaccaaac
    18001 tatgattcgg atgtttggtt gcgaacgttc aaaaaatgct catattgaaa aaaagaaaag
    18061 caagtgcgat tattttttgc ctcaacctcc caccgaagct caaatttttg aattaaccag
    18121 aagagtcaac gaggtaatta atcaaaattt gcctattaga gttgaatata tgacacatga
    18181 agaagcagct aaaatagttg atctctcaaa attaccaact gctactcctg atttattaag
    18241 aattgttcat gtaggtaatt atgatgcttg tgcttgtatc gggcagcatg tgcagaatac
    18301 ttcagaaatc ggacgttttg aaattgttag tcataactat gaaaatgggt gttggagagt
    18361 tcgttttcgt ttggtttgac gtgcctcaat aatcattttt cacaatataa tgaaacgatt
    18421 tggtaaattt ttaaaaaatt tttttgttct atttgtcatc atctcagttg gatgggtaat
    18481 tttttatcgc tttgtgcccg ttttttttac gccgttaatg ctgattcgtt ctgtcgaagc
    18541 aatctcagac ggtgaggctc ctaaaattaa taaaaaatgg gtttcaatag aaaaaatttc
    18601 acccaacatg atacaagctg ttgctgcagc cgaagacgac cagtttatgg aacatcacgg
    18661 tttttctttt gaagccattc ataaagctat ccaacacaac aaaagaagca gacgtattag
    18721 aggcggaagt actatttccc aacaaacggc taaaaatgtt tttctttggc ctcatcgttc
    18781 ttatttacga aaagggcttg aagcttattt tacagtttta atagaattat tctggacaaa
    18841 agaaagaatc atggaagttt acctgaacgt cattgaaaca ggtgatggaa tttatggtgt
    18901 ggaagcagct tcactagaat attttggcaa acacgcttca aaactaacaa agcctgaagc
    18961 ggctttgata gcagcttgcc ttccaaatcc ccgacgtttc aatcctgctc atccttctcc
    19021 ttatatttta cgtcgtcaag ctgctattat gagaatgatg gaaaaaattt caccggtaga
    19081 atttcataaa acgtctgtat ttaaaaaaac caaacataaa catcgataaa aaccaaatga
    19141 acaaacaaat tcaatacctt gactggggaa tcattgaata tgaagaagct ttaagaaagc
    19201 aagaaattct tttttcagat acgattgaaa gaaaaaaaag tggactttct acttcgaatt
    19261 atttcatttt ttgtgaacac ccgtctgtct ttaccttagg gaaaaatggg caagcaaata
    19321 acttgcttat ttcagccaat gaactaagaa aaataaatgc tactttttct ttgaccaatc
    19381 gtggcggaga tattacctat catggacctg gacaaatagt aggttatact atttttgatc
    19441 tggaaaattt tcaattggga ttaaagcaat ttgtcgaaaa aatagaagaa accatcattc
    19501 aacttttgaa actttaccaa atatcagcaa ccagacttaa aggtgctaca ggtgtgtggc
    19561 tggatgctgc gactgagaga gtaaggaaaa tttgtgctat tggacttcgc agttctcatt
    19621 ttatcaccat gcatggtttc gccttaaatg taaatacaaa tctggattat ttcaattata
    19681 ttcatccatg tgggtttgta gataaaggag ttacttcaat ggaaaaagaa ctaaagcaga
    19741 aagttgatat tgttcaagtg aaagaaaact taaaacaatg tttagaggaa caatttactt
    19801 gatatattga aaactaaaaa ctaacttttt tgaaacttgt gcattcaatt gatattaaat
    19861 atacatacta ctgttctact aaattttctc aataaaagtt gatatttcgt ttaaatttaa
    19921 aaattcttcc acttaatcag ggctttttta tgcaaggtta ttacaaaatt gagtaaaaaa
    19981 atatggactt ttttcttata tttacattac aaataaattt tctttgaaaa caataaaaat
    20041 cgttgtaaaa aattagtaaa tgaaatatgt ggaagataat catttctgtt atcccaatgt
    20101 ttgtgtgtct tttctggtgc atagttttgt ttattgaaca tcacacacaa aataaagcaa
    20161 aaaccttcct atcatttttt atgtttgttg cttttttatt atatcttgga catgcagctt
    20221 attttaataa agaatataaa ttttatggtt tttgggatag catctatttg ttttgttcat
    20281 tggcagttta tcccctttat tttatttacc tgaaaattat tactaaaaaa acaaaattaa
    20341 attttaaaga tttttttgta ttactcccag cttttactat tggaattttt tcttttattc
    20401 tttatttcct gatgtcgccc gaagagttga ccgtctatac ccaacaacta atctatcata
    20461 attttaataa cattgaatgg acatcaattc ttcatttgca acaaataaaa ctttacttgt
    20521 tcaccattat atttgttatt caaattattc ctattctgat atatggcagc aaatatatta
    20581 aagaatacaa tcaagctatt atgaattatt atgctgatac agagaaaaag acgctggatc
    20641 attttaattt tttattaatc gtttttattc tcacttcttt agcttcatca acagtagatt
    20701 taataggtag atcttatttt acccagtcca atatccattt gctcattcca gcaattattt
    20761 ttggattttt actttttatg gtcggatatt taggatataa acaagatttt tcaatagaaa
    20821 atttcttaaa ggatattaag aataacgaag ctttagcacg cactataaaa ataagtatta
    20881 atgataccga gctctatgga aaaaatttat ttgaaaatct aaaaaaatta ttagaagaaa
    20941 atgaaatcta taaaaataag gatttatgta ttactgatat tgccataatg ctgaatacca
    21001 acagaactta catctcaaaa atcatcaatg aaaagtttaa aaccaatttt tcaaccatga
    21061 ttaatcatta tcgaatagaa catgcaaaaa aaatactgaa agattctaaa taccgacaac
    21121 ttagcatcaa tgaaataggt gaactttcag gattttcaag cgaaagctct ttttatcgta
    21181 tttttaaaaa ccatgaaaat atttctcctg gtaattaccg gaaaaagcat ctaatcaatt
    21241 attaatttac agatataaaa ttttaaagtt aagagaattg ttacattttt ttaacaacct
    21301 tttgccttac caaaagaagt aaattgtgtt atttcttagc ataaagtaaa tttgaagagt
    21361 aaaggatata ttcataattt tgcaatctat tgtatttgat tattataact gataatattt
    21421 aaaaaacgta aattcaagta attaatgcaa ctttttcagg ggaaataata aggtaaag
//
LOCUS       DAOSAS010000011        21259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_1766_length_21259_cov_14.369836, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000011 DAOSAS010000000
VERSION     DAOSAS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21259
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1766_length_21259_cov_14.369836"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..2051)
                     /locus_tag="PLR72_01095"
     CDS             complement(324..2051)
                     /locus_tag="PLR72_01095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOK35841.1"
                     /translation="MKNYIVSARKYRPITFKSVVGQTALTNTLKNAIKTNHLAHAYLF
                     CGTRGVGKTTCARIFAKTINCEHLTEDYEACNECESCVAFNEQRSYNIHELDAASNNS
                     VDDIRSLIEQVRIPPQIGKYSVYIIDEVHMLSTSAFNAFLKILEEPPAHVIFILATTE
                     KHKIIPTILSRCQIYDFNRISVHEIVEQLQYIAQQENITADINALNVIAQKSDGSMRD
                     ALSIFDQMVSLCENNITYQQVLENLNILDYEYYFQLVNAFLKGDISQSLLIFNEILNK
                     GFDAYHFITGLSSHLRDILVCKDPQTIPLLEVGADIGKHYQTQAESCSSDFLFNALKI
                     TSECELNYNISKNKRLLIELTLIRLCQLTKESQPIIEEKIPLQKIIPNDNAINSKNYQ
                     KDEIQIHNEKMNSRNSVKETNNVSVKEPKQKYIQPPVSISINSSNTTSEEISEKKVTP
                     EIIQNENFTDENLVKAWKDFAETLSEEEIRMKTFIQNTEPQRLSETDFEVSVANTLQE
                     KELKKLQPQILSYLQNQLKNSTIQMKIKLMKKTESQKPKNPEERYKTMAEQNPAITVL
                     KNGLQLEIN"
     gene            complement(2989..3879)
                     /locus_tag="PLR72_01100"
     CDS             complement(2989..3879)
                     /locus_tag="PLR72_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOK35842.1"
                     /translation="MNVLTDSPNIYLVLLILFLLLVSGKTKSQSFDMALSAVGSLKDT
                     TNIYKQMFNDDSDDLMENHPADDIYNNIWSHERINPYNLSIDSLPDSVLIDCSNFVLP
                     IAGKVTSKFGPRRYRYHYGIDLRLNVGDSVKTAFGGKVRIIDYEPGGYGNYVVIRHDN
                     GLETVYAHLSKVLVEVNQDVSAGEMIALGGNTGRSTGPHLHFETRYLGHAINPAEIID
                     FENGELYDQTFLIAKNKTFRYQKERIPQGTLAKYHRVVKGDTLSFIAARYNTTVNKLC
                     KLNRISSQKILRPGEKIRVR"
     gene            complement(3890..4351)
                     /gene="ndk"
                     /locus_tag="PLR72_01105"
     CDS             complement(3890..4351)
                     /gene="ndk"
                     /locus_tag="PLR72_01105"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOK35843.1"
                     /translation="MEKTLVIIKPGAIQRELIGEVITRLERKGLHLCGIKMMQLTDDI
                     VSEHYAHLADLPFFDRIKNSMMVTPIIVCCWEGVEAVKVVRAIVGVTNGRDAQAGTIR
                     GDFSMSIQENIIHASDSVETAKIEIARFFKNDEIFDFKQNNLPFIYANDEK"
     gene            complement(4440..6536)
                     /gene="recG"
                     /locus_tag="PLR72_01110"
     CDS             complement(4440..6536)
                     /gene="recG"
                     /locus_tag="PLR72_01110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOK35844.1"
                     /translation="MDFFNQDIQYVKGVGPKRAELLNKELNIFTVEDLLRHYPYKYVD
                     RSRFYFLNEISENMPFIQLRGKILSFEKAGEGRKQRLIATFTDGFGIMEMIWFNGLRF
                     VQDKYKTNIEYIVFGKPTFFNGKISIIHPEIDLPSKLPPPEQMGMQPFYNTTEKMKSH
                     SLNSKAIQKIIYSLLPTLQNSIPETLPSYLLQRFKLLNLTEAIRNIHFPQNSHLLNKA
                     RQRLKFEELFYIQLSIVRQQKWREQKFNGFIFKHIGYFFNTFYHKYLPFSLTNAQKRV
                     IHEIRKDVGSGKQMNRLLQGDVGSGKTLVALMAMLMAADNDFQACMMVPTEILAQQHY
                     TSIKELLGDLGVEVSLLTGSTRQRERKVIHQKLQNGEIHILIGTHALIEETVQFKNLG
                     LVVIDEQHRFGVAQRAKLWTKNQQPPHILVMTATPIPRTLAMTLYGDLNISSIDELPP
                     GRKPVQTFHFYENKRAQLNKFIMQQVEAGRQIYVVYPLIQESEVLDLKNLEEGYCQLC
                     ETFPQYKISMVHGKMKSDEKELQMKKFVSGETQIMVSTTVIEVGVNVPNASVMIIENA
                     ERFGLSQLHQLRGRVGRGAEQSYCILLTNYKLSATTRKRIEVMIRTNDGFEIAETDLQ
                     LRGPGDLEGTQQSGLPFELKIANLAQDGKMLEIARQTALEILEVDPNLEKPENKVLLK
                     QLKKMKNSPINWGIIS"
     gene            complement(6551..7273)
                     /locus_tag="PLR72_01115"
     CDS             complement(6551..7273)
                     /locus_tag="PLR72_01115"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOK35845.1"
                     /translation="MSRIALIVAGGKGERMKTSIPKQFIELQGKPVLMHTMNVFFRYD
                     PALSFFVVLPSEQIDLWKLLCQQHHFDIPHQIVGGDETRFHSVKKGIEAISHQLQKNK
                     KITNKKNVILVAIHDGVRPFVSTDTIDRCFEEAEKEGNAIPAVELTDSIRKVSLLGNE
                     AEERRNYRLIQTPQVFEFSLLQKAYQQQYSPFFTDDASVVEAMGVNIHLVEGNCENIK
                     ITTSFDLELAEFLLSRNESNNK"
     gene            7436..7903
                     /locus_tag="PLR72_01120"
     CDS             7436..7903
                     /locus_tag="PLR72_01120"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOK35846.1"
                     /translation="MHQLFQIFIVTLLFSLPIFSLSGQQKENIFEVLSKNDSTEGTVV
                     FHQDKRIETLLTEKRIPTIPAKTTVVNGYRVQVFSSNEPRTAKNEAFNIERLINETFP
                     EYGVYVTYSSPFWKVRVGDFASMEEAQAFKNELIEVFPRLRNQTYVVRDQINK"
     gene            7922..8518
                     /gene="folE"
                     /locus_tag="PLR72_01125"
     CDS             7922..8518
                     /gene="folE"
                     /locus_tag="PLR72_01125"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOK35847.1"
                     /translation="MDSELNGGKIFDEKKTNEIAQHYKNIISLLGENPERPGLVQTPE
                     RVARAMQFFLQGYEEDPEEIIREAMFTENYRQMVIVKDIDFYSLCEHHLLPFFGRAHV
                     AYIPNKKITGLSKIARVVEIYARRLQVQERMTTQIKQCIQNTLEPMGVMVIIEAQHLC
                     MQMRGVQKLNSITTTSDFTGVFQEARTREEFMNLIKNR"
     gene            complement(8603..9424)
                     /locus_tag="PLR72_01130"
     CDS             complement(8603..9424)
                     /locus_tag="PLR72_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOK35848.1"
                     /translation="MGKKLFENNSSTKAVGICLSGGGALGFAHIGVLQSLEEHGIYPL
                     QVAGSSMGAVVGVLYAAGISPLKMLQIIKEDKLYRITKLMNIRLTSINSGLSTHDTLR
                     EIIRELIPHNSFDKLPKKMNVCVSNLNTADWEIVNTGENLDKWVAASASIPGIYEAMQ
                     MNKCFYVDGGLLNNFPAQALEHSCQKIIGVDVIPHFETLQLKKPTDTFSWSVRIMEHK
                     NSLPGRKLCHFLIEPMAIKKYHGFSFDAYQKIYEEGYKSTNNYIEKNPEILNLKD"
     gene            9639..11813
                     /locus_tag="PLR72_01135"
     CDS             9639..11813
                     /locus_tag="PLR72_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOK35849.1"
                     /translation="MKRIFFSLILLFSVQISFANLLYDIVDGKFKVKDLVVPYSMKDG
                     ENYTLMVNKKAIVKFNYKTGAVIDTLFSVSRLQNAPFKEIEGYEFSPNEAKLLVRTNV
                     KKRYRRTFIADYYLYDIKRNEIQPLSEYGPQESPLFSPDSRYVAFARNNNLYLKKLDF
                     NTESAITKDGEKNKIINGVPDWLYEEEFGVVRCFEWSPDSKLLAFIKFDETEVPEFSY
                     QLYADSTKMIDGLSLYPTTVTYKYPKAGQNISKVSVCIFDERQKSIRTIELNTLEKNF
                     YIPRIKWTSSVDQLAIFTLNRNQNEMKMFLANPKSTLSKLILQEKDDNYIDYRNFDFF
                     QFSSDNQYFIGVSEKDGYRHVYQYQINGTLIKQLTAGNWDVTDVYGFDENTQTLYYQS
                     AETSPLQRDVYAITTKGKKTRLTDGKGVHQAIFNSNFTYFVDNASSLENANTLTVCNN
                     KGNFIRVFQNNDSITQEWEKLNLPKKEFFSFTTSENIKLNGWILKPSNFDTTKKYPLL
                     LVQYSGPDAQEVLDEWNVDWEYYLATQNYVVACVDGRGTGARGVKFRKCTYQQLGILE
                     TKDQIETAKFLSSQHYIDNERIGIWGWSYGGFITLMAMSSPEKIFKTGIAVAPVTDWR
                     LYNAAYTERYMRRPQENFKGYDLTSPLLHATHLNGNLLIIHGTADDNVHAQNTLLYAQ
                     HLEENNKKFQMLLYTDKNHSILGKETRRHLYQQMSEFLFMNL"
     gene            11999..13351
                     /locus_tag="PLR72_01140"
     CDS             11999..13351
                     /locus_tag="PLR72_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK35850.1"
                     /translation="MATQKKNGNLVAIITMIFLFGMISFVTNLAAPIGVIWKNQPQFE
                     GSNFLGMLGNMMNFLAYLFMGIPAGKLLTRIGYKKTALAAIAVGFFGVLIQYLSGIIG
                     VGSQLFALPANFFVYLLGAFVAGFSVCMLNTVVNPMLNLLGGGGNKGNQLIQIGATFN
                     SLAATLTPMFVGALIGDITKERVLSGEITINSVNPILYIALGVFAATFLILLFIPIKD
                     PDENRSRNIEIKYEHSPWNFRHFVLGAIAIFIYVGVEVGIPGTLNFFLADVSDKGAAL
                     NPQKAAAIGGFVAGTYWFLMLIGRFIGSLIGGKISSKTMLTSVATGGIILVLLAIFLP
                     KNLTTSMPVFTGSSFGMVQVPLIALFLVLCGLCTSVMWGCIFNLAVEGLGKYTAAASG
                     IFMMMVVGGGILPLLQNWIADISTYQTSYWVIFVGLLYILFYAVIGSKNVNKNIPVEI
                     "
     gene            13375..14331
                     /locus_tag="PLR72_01145"
     CDS             13375..14331
                     /locus_tag="PLR72_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOK35851.1"
                     /translation="MEKPYVIGIDMGGTNTVFGIVDVRGNVLSKSAIKTTAHSDVNLY
                     IDDLYNELMKLIVAEGGIEKIRGIGVGAPNGNFYTGNIEFAPNLPWKGIIPFSSLLRE
                     KFGIPVALTNDANAAAIGEMTYGIARGMKDFIMITLGTGVGSGIVIDGKLVYGHDGFA
                     GELGHVTAIRNGRLCGCGKNGCLEAYASATGVARTAREILETTNQESILRNIPLADIT
                     SKDVYDAAKEGDEVAIEIFNFTGKILGELFADFVAFSAPEAIILFGGLSKAGEFILGP
                     IRENMEKNLLPIWKGKIKILFSILKESDAAILGASALAWELQ"
     gene            14707..15111
                     /locus_tag="PLR72_01150"
     CDS             14707..15111
                     /locus_tag="PLR72_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35852.1"
                     /translation="MPTEDYLLKYLEKLNYAIAAMLGLREKGFPEDALKMAENTYREL
                     LHYNLKELSIMPIDQFIETIRQKNYNVSILSLLTKLTDETASSFEADHQTETAKIFYL
                     KSLELYHLLNEKDKTFSFEREMRIQELENEIL"
     gene            15152..15925
                     /gene="rlmB"
                     /locus_tag="PLR72_01155"
     CDS             15152..15925
                     /gene="rlmB"
                     /locus_tag="PLR72_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOK35853.1"
                     /translation="MINQYKTRRRPEKEMIFGVRAVIEAIQSGKEIEKILLRKNISNE
                     TRRQLFQTIKDLQIPVQFVPEEKLNTITNKNHQGVIAYISPISYQSIENIIPQIYEEG
                     RMPFVVVLDEITDVRNFGAIARTCEGGKVDAIIIPARGSASINADAIKTSAGALFSMP
                     ICRANNLENTLKFLLDSGLKLVAATEKADKNYTEVSYKEPIALVMGAEDKGIRSELLS
                     LCEEKIRIPMLGKIESLNVSVAAGILIYEVVRQRNLNSI"
     gene            15953..16639
                     /locus_tag="PLR72_01160"
     CDS             15953..16639
                     /locus_tag="PLR72_01160"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOK35854.1"
                     /translation="MEILYEDNHLIAINKINSDIVQGDKTGDEPLSEKVKKYLKEKYN
                     KPGAVFLGVPHRLDRPVSGVVLFAKTSKALSRLNVMFQNQEIKKTYWAIVRQKPPHRE
                     GQLEHFLVRNEKLNRTFAYDEPVNDSKKAILSYKILANSDYYYLLEVHLKTGRHHQIR
                     CQLAKIGSPIKGDLKYGYPRSDKNGGISLHARKIEFVHPVSNQLIQITAPVPQDDNLW
                     LYFENLQENK"
     gene            16647..17492
                     /gene="lipA"
                     /locus_tag="PLR72_01165"
     CDS             16647..17492
                     /gene="lipA"
                     /locus_tag="PLR72_01165"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOK35855.1"
                     /translation="MNNLQKPDWIRTKLTSTSNFSTVHQTIQQHRLHTICESGKCPNL
                     SECWNRGTATFMILGEICTRSCKFCNTATGRPLPPDPTESQAIAEAIYSLKLKHAVIT
                     SVDRDDLPDKGASFWAKTIHAIQAKNPHTTIEVLIPDFDGQHDLLDKIIQEKPDIISH
                     NMETVRRLTPLIRSKARYETSLAVLNYVASQGIITKTGIMVGLGETEEEVLELMDDVL
                     NIGCSILTIGQYLQPSKKNIPVSAYISPQQFEKYKEIGLKKGFKYVESAPFVRSSYFA
                     EKHII"
     gene            complement(17524..18270)
                     /locus_tag="PLR72_01170"
     CDS             complement(17524..18270)
                     /locus_tag="PLR72_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOK35856.1"
                     /translation="MTQSLIFTLLALFLGSVGSVLLAGLILLLKEKRLQRITRFLTYL
                     AGGTLLGSALLGMIPEALAVSKPEIVLNVLLGGLVFFFVLEKIILWRVCNDASCERNL
                     SAAAPMVLFGDAFHNAIDGVIIASAFLTSNSFGWVVTLSVVFHEVPQELGDFGVLLRS
                     GMSRSKALFYNALSGSTALALGIIAYFSLSFAKSLIPYALAFSAASFLYIALADLIPE
                     MHRKTSFKEGLIQITFILTGILLIYLFGRD"
     gene            complement(18267..19142)
                     /locus_tag="PLR72_01175"
     CDS             complement(18267..19142)
                     /locus_tag="PLR72_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOK35857.1"
                     /translation="MQESGINEQCKFCFVQNFERLIEKFPINESDREKFEKHYEKILR
                     ESDGKLMPEIYRELNKEFLKLSGLSDPFAEEKRKSNEIALELYNDLKEVVEKSENPFQ
                     LALRLAIAGNIMDYGPTAHFDVHSTIEKAFTIQFAIDHSAELEKRIKIAKKILYLGDN
                     AGEIVFDKLFIETMKHPNVTFAVRGAPALNDVTLEDAYQVEMDKVARVISNGNDVAST
                     IVKECSSEFQEIFQEADLIISKGQGNLEGLIYQNDPRIFFLLVAKCDVIAQLLGVEKG
                     SFVVYNTQLPKNKNL"
     gene            complement(19186..19650)
                     /locus_tag="PLR72_01180"
     CDS             complement(19186..19650)
                     /locus_tag="PLR72_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOK35858.1"
                     /translation="MNVSSKFFVILPCFKKSKKIMARPQHQRKIASPPLMMGYQPFGI
                     FPKESVKIILHLDEYEALRLVDYEGLQQTEAAKKMQISRPTLTRIYENARKTIAKAMV
                     EGKTIVIEGGNVEFDQQWFRCKHCFKLVNEKETHIHTNEEEKENLLPLLTNK"
     gene            19921..21210
                     /locus_tag="PLR72_01185"
     CDS             19921..21210
                     /locus_tag="PLR72_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOK35859.1"
                     /translation="MVNSLLQNLYTRQDIEITAPAGSWESLMAAVKAGADSVYFGIEK
                     LNMRSKSSNNFTTEDLPHIVNICKENKVKSYLTVNTILYDSDLELMRKIIDLAKKENV
                     DAIIASDVAAMIYANSIGMEVHLSTQLNISNIEALKFYAQFADVVVLARELNLNQVLE
                     IYQTIQKEKICGRSGRLIRIEMFCHGALCMAVSGKCYLSLHEKNLSANRGECNQICRR
                     GYLVKDKDSEIELEIDNEYIMSPKDLKTISFIDQMLEAGVRVFKIEGRARSAEYVKTV
                     VSCYKEAIESCLDGTYNEEKIKNWDKKLSRVFNRGFWNGYYLGQRLGEWNKNYGSAAT
                     HKKIYIGKVTNYFSKIGVAEFLLETQSLSVGDEILITGETTGAYEDTVTEIRVEDVPV
                     EKVEKGTYFSMKTNELVRRNDKLFKIVPTKHGKEERE"
BASE COUNT         7154 a         3424 c         3548 g         7133 t
ORIGIN      
        1 ataatctttt ttctttatga aattgataaa gataagatga atgtattgtg tgttaacact
       61 tctttcctta cctgacatct ttctaaaact aaaagaaagg aatttgttac aatccacttt
      121 ctatgaaagg gatgtcaact atcagctgat agagtaatta aaaaatcatt tcctcatacg
      181 gatttaattc tacgatacaa atttattttg tataaatatt cgtattttaa taattttgat
      241 aacagtttag tttggtatta tttcttttgt ctattaaaag acaaaaagaa cagataagaa
      301 atttctatcg ttcttagggt aatttaattt atttcgagtt gcaaaccatt tttaaggacc
      361 gtaattgcag gattttgttc agccatcgtt ttatagcgtt cctcaggatt ttttggcttt
      421 tgcgattcag tttttttcat taatttaatt ttcatttgaa tggttgaatt ttttaactga
      481 ttttgcaaat aacttaaaat ttgtggttgt aattttttta attctttttc ctgaagagta
      541 ttagcaactg aaacttcgaa atctgtctcc gataagcgtt gaggttctgt attttgaata
      601 aaagttttca ttctgatttc ttcttcagaa agtgtttcag caaaatcttt ccaggctttc
      661 accaaatttt catccgtaaa attttcattt tgaatgattt caggggttac ttttttttct
      721 gaaatttcct ccgaagttgt attagaagaa ttgatagaaa tgcttacggg tggttggata
      781 tatttttgtt ttggttcttt cactgaaaca ttattagttt cttttacaga atttcttgaa
      841 ttcatttttt cattatgaat ctgaatttcg tccttttgat aattttttga atttatagca
      901 ttatcgttag gtataatttt ttgtaagggt attttttctt caataattgg ttgactttcc
      961 ttagttaatt ggcacaaacg tattagcgta agttctatca acaatcgttt gtttttgctg
     1021 atattataat taagttcaca ttcactggtt atttttaagg cattgaataa aaaatcagaa
     1081 gagcaacttt ctgcctgtgt ttgataatgt tttcctatgt cagcacccac ttccagtaaa
     1141 ggaatagttt gtggatcttt acaaaccaaa atatcgcgca aatgagaact aagtcctgta
     1201 ataaaatgat aggcatcaaa tcctttgttc aaaatttcat tgaaaatcag aagtgattga
     1261 ctaatatctc cttttaaaaa agcatttact aattgaaaat aatattcata atccagaatg
     1321 tttaaatttt ccaacacttg ttgatatgta atattatttt cgcacagact taccatctga
     1381 tcaaaaatag ataaagcatc acgcatgctc ccgtcacttt tttgagctat cacattaaga
     1441 gcattgatat cagccgtgat attttcctgt tgagcaatat attgtaactg ctcaacaatt
     1501 tcatgaacag aaattctatt aaaatcataa atttggcaac gagagagaat cgttggaata
     1561 attttatgtt tttctgtggt tgccagaata aatataacat gtgcgggtgg ttcctccaga
     1621 atttttagaa aagcgttgaa ggcactggtg gaaagcatgt gaacttcgtc aatgatatac
     1681 acgctgtatt tccctatttg gggcggaatg cgaacctgtt caattaagga acgaatatcg
     1741 tccacactat tattggaagc agcatccagt tcatgaatat tgtaggaacg ttgttcgttg
     1801 aaagcaacgc atgattcaca ttcattgcag gcttcataat cctctgtaag atgttcacaa
     1861 ttaatagttt ttgcaaaaat ccgtgcacag gtggttttgc caacgcctct ggttccacaa
     1921 aacagatagg catgtgccag atgattggtc tttatggcat tttttaaagt gtttgtcaaa
     1981 gccgtttgtc caactaccga tttgaaggtg attggacgat attttcttgc tgaaacaata
     2041 tagtttttca taaacaaatt tttcaatctt ttacttgcaa atttaatgaa aaaaatttag
     2101 gttttgaacc cgtgaaattt tttttgaaag aaataaaaga ttataaagaa cttgttatca
     2161 ttgggttgat attatttatt aggaaaattt aactgagaat tgtcattaga aatatatcgt
     2221 tcacttaaat ataaagtttt taaagtattg aaaatcattc caattttttt tgcatgataa
     2281 acgatgatat tttttctatt aaagttgcat ttaaccgatt aacgatcttt ttagatatat
     2341 ttcgtctgat gagctgaaaa ttgatcggtt tcgagtttgc gaggtgcagg attcgagata
     2401 cgaggtgcga attttgagtc atgagtttgg agcgaaaaaa gaaaaaatag ctgtgtccct
     2461 ctgtgtaata aatcattcat cttcacaggg tttcacagag aaggcacgga gtggaacaga
     2521 aaaaaatcta aaaaacaaat aaaaatgaga ggagtcgagg caaagtacct aatacaaatt
     2581 cagtaaattt tagacaattt ttatctaggg gctttttata aataagtaag aatttcttac
     2641 tttcattttt gaaaaacaag cttaattttg ctaatgtttt tctcgataca atatattata
     2701 gttcagataa ttatagctta ttttctaatg acaatttggg ttgaaggcaa gtttggacaa
     2761 ggtaaaaacg attataatat gaataaaata agattactct tggcttaccc ctgtaaaagc
     2821 cgggcggcgg gcattgtatt tgtaatgaat atcctctaag tactcaagga tattttttat
     2881 caatttttaa aaactatttt ttattttttt tgcccatgaa agtcattact tccaataggt
     2941 ttacaagaaa atcgcttgag ggaaaaatat ttttttgcaa atcctaattt aacgaactct
     3001 tattttttct cctggtctta atattttttg agaagaaatt ctattcaatt tacataattt
     3061 attcacagta gtattataac gagctgcaat gaaactgagt gtatctcctt taacaacgcg
     3121 atgatattta gctaacgttc cttgggggat tctttctttc tgatatctga aagttttatt
     3181 tttagcaatc aaaaaggttt gatcatataa ttcgccattt tcaaaatcaa taatttcagc
     3241 aggattaata gcatgtccta aatatcgtgt ttcaaaatgc aggtgaggtc ctgtcgatct
     3301 gcccgtgttt cctcccaatg caatcatttc tcctgcactc acatcttgat tcacttctac
     3361 caacactttt gacaagtgag cataaacagt ttctaaacca ttatcatgac gaattaccac
     3421 ataattacca tatcctcctg gttcataatc tataatgcga actttccctc caaaagcagt
     3481 ttttactgaa tcacctacat ttaaacgcaa gtcaattcca taatggtaac gataacgacg
     3541 aggaccaaat tttgatgtta ctttccctgc tattgggaga acaaaattgc tacaatcaat
     3601 cagaacggaa tcaggcaaac tatcaataga caaattgtaa ggattgattc tttcatgcga
     3661 ccagatgttg ttataaatat cgtctgctgg gtgattttcc attaagtcgt ctgaatcatc
     3721 attaaacatt tgtttataaa tattagttgt gtcttttaaa gaaccaacag cacttaaagc
     3781 catatcaaaa ctttgtgatt tggtttttcc gcttactaaa agtaaaaaga gaatcaacag
     3841 tactaaataa atgtttggag aatcagttaa cacattcata actcagcaat tatttttcat
     3901 catttgcata tataaaaggc aaattatttt gtttaaaatc aaaaatttcg tcgtttttga
     3961 aaaaacgagc aatttctatt tttgcagttt caacactatc agatgcatgg ataatatttt
     4021 cttgaatgct catactaaaa tcaccacgga ttgttcccgc ctgagcatct cgtccatttg
     4081 ttacacccac tattgctcgc actactttta ctgcttctac tccttcccag caacaaacga
     4141 taataggagt aaccatcata gaatttttta ttctgtcaaa aaagggaaga tcagccaaat
     4201 gggcataatg ttcagaaaca atgtcatcgg ttagttgcat catctttata ccacacaagt
     4261 gtaaaccttt tcgttccaat ctggttatca cttcaccaat caactctcgc tgaatggctc
     4321 ctggcttaat gatgacaagc gttttttcca tttaaaaaag ttgttaaact tctcttttta
     4381 tagaaatcat tccgaaagta aaaaaatgaa attttaaatc caaaataatt taaggttttt
     4441 taactaatta taccccaatt gatgggacta tttttcattt ttttcagttg cttcaacaaa
     4501 actttatttt ctggcttttc taagttggga tcaacttcca aaatttccag agctgtttgc
     4561 cgagcaattt ccaacatttt accatcttga gctaaatttg ctatttttag ttcaaagggc
     4621 aaaccacttt gttgcgttcc ttccagatca ccaggtcctc ttaattgtag atcggtctca
     4681 gcaatttcaa aaccatcgtt agtccttatc atcacttcaa tgcgtttgcg agtagtagct
     4741 gataatttat aattggtcaa taaaatacaa taggattgtt ctgctccacg acctactcgt
     4801 ccacgtaact ggtgcaattg cgacaaacca aatcgttcag cattttctat aatcatcaca
     4861 gaagcatttg gaacattaac acctacttcg atgacagtag ttgacaccat aatttgagtt
     4921 tctcctgaaa caaatttttt catttgaagt tctttttcgt ctgatttcat tttcccatgc
     4981 accatactaa ttttatattg tggaaatgtt tcgcacaact ggcaatagcc ctcttctaaa
     5041 ttttttaaat ccaatacctc cgattcttga ataagtggat aaactacata gatttgtctt
     5101 ccagcttcaa cttgctgcat aataaatttg tttagttgag ctcttttgtt ttcataaaaa
     5161 tgaaaagttt gaacaggttt tctgccgggt ggcaattcat caatggaaga aatatttaaa
     5221 tctccataaa gcgtcattgc caacgtacgt ggaataggcg tagcagtcat gacaagtata
     5281 tgagggggtt gctgattttt agtccaaagt tttgctcgct gagcaactcc aaaacgatgc
     5341 tgttcatcaa taactaccaa acccaaattt ttgaattgaa cagtttcttc aatcaaagca
     5401 tgggtgccaa ttaaaatatg aatttcaccg ttttgtaatt tttgatgaat aacttttcgt
     5461 tccctttgac gtgtagagcc agttaacaaa gacacttcaa cacctaaatc gcccaataat
     5521 tccttaatgc tggtataatg ttgttgagcc aaaatttcgg taggaaccat catgcaagcc
     5581 tgaaaatcat tgtcggcagc cataagcatt gccattaaag caaccagtgt ttttccactt
     5641 cctacatccc cttgtaatag acgattcatt tgtttgcctg aaccaacatc ttttctgatt
     5701 tcatgaataa cccgtttttg agcattggtc agggagaaag gtaaatattt atgataaaaa
     5761 gtattgaaaa aatatccaat atgtttgaaa atgaatccat taaatttttg ttctctccat
     5821 ttttgctgac gaacaatact tagctgaatg taaaacagtt cttcaaattt caatcgctga
     5881 cgggctttat tgagtaaatg tgagttttga ggaaaatgaa tgtttctaat ggcttccgta
     5941 agatttaaga gcttgaatcg ttgtaaaaga tacgaaggaa gtgtctcagg gatactgttt
     6001 tgtaaagttg gcaagaggga ataaataatt ttttgaatag cctttgaatt caaggaatgg
     6061 gatttcattt tttcagtggt attgtaaaaa ggttgcattc ccatctgttc aggcggcggt
     6121 aattttgaag gaagatctat ttcaggatgg ataatactga ttttgccatt aaagaaagtt
     6181 ggcttcccaa aaactatata ttctatattg gttttatatt tgtcttgaac aaaccttagt
     6241 ccattaaacc aaatcatttc cattatgccg aagccatcag taaaagtagc tattaatcgt
     6301 tgcttccgtc cttctcctgc tttttcaaaa cttaaaattt tacctcttaa ttgaataaaa
     6361 ggcatatttt ccgaaatctc atttaaaaaa tagaaacggc ttcgatccac gtatttataa
     6421 ggataatgtc tcaataaatc ttcaacagtg aaaatgttga gttctttatt cagtaattcg
     6481 gcacgtttgg gaccaacgcc ttttacatat tgaatatctt gattaaaaaa atccaattcg
     6541 atcaaaaaat ttacttgtta tttgattcgt ttctgcttag taaaaattca gctaattcca
     6601 aatcaaaaga agtagtaatt ttaatatttt cgcaattacc ttctaccaaa tgaatattaa
     6661 cacccatagc ttccacaaca gaagcatcat cagtaaaaaa aggagaatat tgctgttgat
     6721 aagctttttg caaaagtgaa aattcaaata cctgcggagt ttgaatcaat ctgtaatttc
     6781 ttctttcttc agcttcattt cccagaagtg aaacttttct tatgctgtca gttaactcaa
     6841 cggctggaat ggcattccct tccttttcag cttcttcaaa acatctatcg atggtgtctg
     6901 tactaacaaa aggacgaaca ccatcatgta tagcaactaa aataacattt tttttattcg
     6961 taattttctt attcttttga agttgatgag aaatagcttc tatacctttc tttacagaat
     7021 gaaagcgtgt ttcatcaccg ccgactattt gatgagggat atcgaaatga tgttgttgac
     7081 aaagcaactt ccagaggtca atttgttcag atggaagtac aacaaaaaaa gataaagcag
     7141 gatcataacg aaaaaaaaca ttcatagtgt gcataagcac aggtttccct tgcaattcga
     7201 taaattgttt gggaatagaa gttttcattc gttcgccttt tccaccagct actatcaaag
     7261 caatcctgct aatattcata aaaattgttc tgttatcttt aaacaaaaat aaaaaaattt
     7321 tcaggaaaaa ctcttaaaat tttattgaga tttgtcaaag ataaagaaat taatcttaat
     7381 ggtagttata aaagaaacat tttgtaactt tgcaaccata tatcatttca ataaaatgca
     7441 ccaactattc cagatattta tagtaacatt gctattctct cttcctattt tttctttaag
     7501 cggtcagcaa aaagaaaaca tttttgaagt actttcaaaa aacgacagta cggaaggaac
     7561 tgttgttttt catcaagaca aacgtatcga aactctttta acagaaaaga gaattcctac
     7621 tattcctgct aaaactactg ttgtcaatgg ttaccgtgtt caggtttttt caagtaatga
     7681 gcctcgaaca gctaaaaatg aagcttttaa tattgaaagg ttaattaatg aaactttccc
     7741 ggagtatggt gtttatgtta cttattcttc gcctttctgg aaagtgcgtg tgggtgattt
     7801 tgcctccatg gaggaagctc aggcttttaa aaatgaatta attgaggttt ttcctcgatt
     7861 gcgaaatcag acttatgtag ttagagatca aattaataag taatttttca aataaattta
     7921 gatggatagt gagttaaatg gtggtaaaat ttttgatgaa aagaaaacca acgaaattgc
     7981 tcaacattat aaaaatatta tcagtctttt aggagagaat cctgaacggc ctggattagt
     8041 acaaactcct gaaagagttg cccgagctat gcaatttttt ttgcaaggtt atgaagaaga
     8101 tccagaagaa ataatccgtg aggcaatgtt tactgaaaat taccgtcaaa tggtaattgt
     8161 aaaagacatt gatttctatt cattatgtga acatcatttg ttgccatttt ttggaagagc
     8221 tcacgtagca tatattccta ataaaaaaat aacgggtttg agtaaaatag ctcgtgtagt
     8281 cgaaatttat gcccgtcgtt tacaggttca ggaacgaatg actacccaaa ttaaacaatg
     8341 cattcaaaac acacttgaac caatgggtgt aatggtcatt attgaagcac agcatttgtg
     8401 tatgcaaatg agaggagtac aaaaacttaa ttccattacc accacttcag attttacggg
     8461 tgtgttccag gaagccagga caagagaaga gtttatgaat ctgattaaaa ataggtaaag
     8521 aacttaattg tttttcttaa aaatctataa aatatatatg attgcggtca agcatgagct
     8581 tattttctgg atgaataaaa gtttaatctt tcaaattcaa tatttcagga tttttttcaa
     8641 tgtaattatt ggttgattta taaccttctt cataaatttt ttggtaggca tcaaaactaa
     8701 atccatgata ttttttaatt gccatcggtt caatcaaaaa atgacaaagt tttcttcctg
     8761 gtaacgaatt tttatgttcc ataattctta cagaccatga aaaagtatcg gtaggttttt
     8821 tcaattgaag tgtttcaaaa tgtggaataa catctactcc gataattttt tgacaactgt
     8881 gttcaagagc ttgagctgga aaattattca ataaacctcc atctacataa aaacatttgt
     8941 tcatttgcat tgcttcataa atgcctggaa tacttgctga ggctgctacc catttatcaa
     9001 gattttcacc tgtattgaca atttcccaat cagcagtatt caaattagat acacaaacat
     9061 tcattttttt tggtaattta tcaaaactgt tatgaggaat taattcccga atgatttccc
     9121 gtaatgtatc atgcgttgac aatcctgaat taatagaagt gagacgaata ttcattaatt
     9181 tagttattct ataaagttta tcttctttta taatttgtaa cattttaagt ggcgaaatgc
     9241 ctgctgcata caggacacct accacagctc ccatacttga acctgccact tgcaatggat
     9301 aaattccatg ttcttctagc gattgcaata ctccaatgtg agcaaatccc aacgctccac
     9361 caccacttaa acatatcccg actgcttttg tcgaagaatt attttcaaat aattttttcc
     9421 ccattctttc tgataatgtt ttgtttacaa attaattttc atataaaaaa tcactattgt
     9481 ttcgttactt tatgtggtaa aaaaacaaaa cgaaatttca ataatcaaca aattgcttta
     9541 tttttgtaca attgatatca aatttaatat tttttatgtt caacaaaatt gaacaaaagt
     9601 aaagtagcta ttattcaaag ttaaaaaata aattgaaaat gaaacgtatt ttttttagtt
     9661 taattttatt attcagtgta caaatttcat ttgccaatct actttatgat attgtagatg
     9721 gcaaattcaa agtaaaggat ttagtagtac cgtattctat gaaagatgga gaaaattaca
     9781 ccttaatggt taataaaaaa gccatcgtca aattcaatta taaaactggt gctgtaatcg
     9841 atacactttt tagtgtttca cgattacaaa acgctccttt taaggaaatt gaaggatatg
     9901 aatttagtcc aaatgaagct aaattattag tacgtaccaa tgtaaaaaaa cgctatcgaa
     9961 gaacatttat tgctgattat tacctttacg atataaaaag gaatgaaatc cagccacttt
    10021 ctgaatatgg accgcaggaa tctcctttgt tctcgcctga tagccgctat gttgcttttg
    10081 caagaaacaa taatctatat ttaaaaaaat tagattttaa tactgaatca gctattacca
    10141 aagatggcga aaaaaacaaa atcattaatg gtgtccccga ttggctttat gaagaagaat
    10201 ttggtgttgt tcggtgtttt gaatggagcc cggatagcaa attgttggca tttataaagt
    10261 ttgatgaaac cgaagttcct gaattttctt accaacttta tgccgactct actaaaatga
    10321 tagatgggct gtcgctttat ccaacaaccg taacttataa ataccccaaa gcgggtcaaa
    10381 atatctcaaa agtcagtgtt tgtatttttg atgaaagaca aaaatcaata agaacaatcg
    10441 aattaaatac tttagaaaaa aacttttata taccacgaat taaatggact tcctccgtgg
    10501 atcaattggc tatttttacc ttaaatcgaa atcaaaatga aatgaaaatg tttttagcca
    10561 atcctaaatc tactttatca aaattaattc ttcaggaaaa agacgataat tatattgatt
    10621 atagaaattt tgattttttt caattttctt ctgacaatca atattttatt ggtgttagtg
    10681 aaaaagatgg ttatcgtcac gtttatcaat atcaaataaa tggaacttta attaaacaac
    10741 taactgctgg gaattgggat gttactgatg tttatggttt tgatgaaaat acccagacac
    10801 tttattatca atctgctgaa acgtcgccgc ttcaacggga tgtttatgct attacaacaa
    10861 aaggaaaaaa aacaagatta acagatggaa aaggagttca tcaagctatt tttaatagta
    10921 attttactta ttttgttgac aatgcttctt ctcttgaaaa tgcaaatact ttaacagttt
    10981 gtaataataa aggaaatttc atacgtgttt ttcaaaacaa tgactccata acccaagaat
    11041 gggaaaaact aaatttacca aaaaaagaat ttttcagttt tactacttca gaaaatataa
    11101 aactaaatgg ctggatatta aaaccttcca attttgatac aacaaaaaaa tatcctttgc
    11161 tgcttgttca atatagtggt cctgatgctc aagaagtttt agatgaatgg aatgtagatt
    11221 gggaatatta tttagccact caaaattacg tagtagcttg cgtggatgga cgaggaactg
    11281 gagctcgagg tgtgaaattt cgcaaatgta cttatcaaca attaggaatt ttagagacaa
    11341 aagatcaaat cgagacagca aagtttttga gttctcaaca ttacattgac aatgaacgaa
    11401 taggaatttg gggatggagt tatggaggtt tcattacgct tatggcaatg agtagtccag
    11461 aaaaaatatt taaaacaggt attgctgtgg ctcctgttac agattggaga ttatacaacg
    11521 ctgcttatac agaacgatat atgcgacgac cacaagaaaa cttcaaggga tatgatttga
    11581 cctctccctt attgcatgcc actcatttga atggtaattt attaattatt catggcacag
    11641 cagatgataa tgtacacgca caaaatactt tattatatgc ccaacattta gaagaaaaca
    11701 acaaaaaatt ccagatgttg ctatatactg ataaaaatca tagtattttg ggaaaagaaa
    11761 ccagaagaca tttatatcaa caaatgagcg aatttctatt tatgaatttg taaacgaatt
    11821 ataaatcaaa acaaaaatag tgattgaaac aaaaaatatg ttgtaaaaca ttgtttatct
    11881 gatgtatttt ttgatgttct ttttaagaga tttgacaaaa aaatggtact tttgttggtc
    11941 tatcttttta tttaaactgt aaatttaaca ttattaactt aattaatttc gaaacaatat
    12001 ggctacacag aaaaaaaatg ggaatctcgt agcaattatc acaatgattt tcctttttgg
    12061 aatgatttca tttgtgacta atcttgcagc tcctattgga gttatttgga aaaatcagcc
    12121 acaatttgaa ggctcgaatt tcctgggtat gttaggaaat atgatgaact ttctggctta
    12181 tctgtttatg ggtattccag caggaaaatt gttaacaaga atcggttata aaaaaactgc
    12241 tcttgctgca attgctgtag gattttttgg tgtcttaatc caatatttat ctggaattat
    12301 tggagtgggt tctcagcttt ttgctcttcc tgctaatttc tttgtttact tattgggtgc
    12361 ctttgtggca ggtttttcgg tatgtatgtt gaacaccgtt gttaatccta tgttaaatct
    12421 tcttggtgga ggtggcaaca aagggaatca attaatccaa attggagcaa cattcaattc
    12481 tttggctgct acactaacac ctatgtttgt aggtgcctta attggtgaca ttacaaaaga
    12541 acgtgtttta tcaggtgaaa ttactattaa tagtgtaaat cctatccttt atattgcttt
    12601 aggtgttttt gcagctactt ttctgattct attattcatt cctattaaag atccagatga
    12661 aaatagaagt agaaatattg aaattaaata tgaacatagt ccctggaatt ttcgtcactt
    12721 tgtattaggt gctattgcta tttttattta tgttggagta gaagtaggaa ttccaggtac
    12781 acttaatttt ttccttgcag atgtttctga caaaggagca gcattgaatc cacaaaaagc
    12841 agcagctatt ggaggatttg ttgctggaac atactggttt ttaatgctta ttggtcgttt
    12901 catcggaagt ctgattggtg gtaaaatttc cagtaaaact atgttgacaa gtgtagctac
    12961 aggagggatt attttagttt tacttgcgat tttcttacct aaaaatttaa caacttctat
    13021 gcctgtattt actggttcat catttggtat ggtacaagtt cctcttattg ctttgttttt
    13081 agtactatgt ggtttgtgta cctcagttat gtggggatgt atttttaatc tagcagtgga
    13141 agggcttgga aaatatacag cagcagcttc aggaatattt atgatgatgg tcgttggagg
    13201 tggtatttta ccattattgc aaaattggat tgcagatatt tctacttatc aaaccagtta
    13261 ttgggtaatt tttgttggat tgctatatat tctgttttat gccgtaattg gctcaaaaaa
    13321 tgtaaataaa aatattcccg ttgaaatttg aaaaacaaaa taaaaaattt aattatggaa
    13381 aaaccttatg taataggcat tgacatggga ggaacaaata ccgttttcgg tattgtagat
    13441 gttcggggaa atgtattaag caaaagtgct ataaaaacaa ctgcacatag tgacgtaaat
    13501 ctttatatag acgaccttta taatgaatta atgaaattaa ttgtcgctga aggtggcata
    13561 gaaaaaatta gagggattgg agttggtgct ccaaatggaa atttttatac aggtaacatt
    13621 gaatttgctc ctaatttacc atggaaagga attattcctt tttcctcact tcttcgtgag
    13681 aaatttggga taccagtggc tttgacaaac gatgcaaatg ctgctgcaat cggagaaatg
    13741 acgtatggta tagctcgcgg catgaaagat tttattatga ttactttagg tacaggtgta
    13801 ggaagtggta tcgtcattga tggaaaatta gtttatggtc acgatggctt tgctggagaa
    13861 ttaggacacg taactgccat aagaaatggt cgtttgtgtg gatgtgggaa aaatggttgt
    13921 cttgaggctt acgcatcagc taccggagtt gctcggacag caagggaaat ccttgaaaca
    13981 accaatcaag aaagtatttt acggaatatt cctttagctg atattacttc aaaagatgtt
    14041 tacgatgcgg cgaaagaagg cgacgaagta gctattgaaa tttttaattt tacgggaaaa
    14101 attttaggag aattatttgc tgattttgtt gctttcagtg cccctgaggc tattattctt
    14161 tttggaggtt tatcaaaagc aggtgaattt atattaggtc ctattaggga aaacatggaa
    14221 aaaaatcttc ttcctatctg gaaaggaaaa attaaaattc tattctcaat tcttaaagaa
    14281 tcggatgcag ccattttagg agcaagtgct ttagcttggg aactccaata gaattcttaa
    14341 ttcattgatt ataaaaacca aaggttacaa tttttcttat tgtcatcttt ggtttttttg
    14401 taaattttac tatatcttta tcattgtaat aaatcattca ttttcagaaa attacacaga
    14461 aaatttttat aagaaatttt taatttgcta tgagataaaa atacaaaaag catttttgga
    14521 tttcctctgt tataagtagg aatctgttct taaatttgat ttttaagagt tgactgataa
    14581 aaaagatatt caattatttg agtaactttg caaaaaattg ctcttcaatc aagagaaaaa
    14641 attcaatttt aatcaaaaga attcctcgag gcttgcctcg gggttaatca tttatcaaaa
    14701 tatcagatgc caacagaaga ttatttactg aaatatctag aaaaattgaa ttacgctatt
    14761 gcagctatgt tgggtttacg tgaaaaaggt tttccagaag acgccctgaa aatggctgaa
    14821 aatacttata gagaattatt gcactataat ttgaaagaat tgagtattat gccaatagat
    14881 caattcattg aaacaatacg gcagaaaaat tataatgttt ccattttaag cctcctgaca
    14941 aaattaaccg acgagacagc ttcttccttt gaagctgatc atcaaacaga aacagcaaaa
    15001 attttttatt taaaatcgct ggaattgtat catttactga atgaaaagga caaaactttt
    15061 tcctttgaac gagaaatgag gattcaggaa ctggaaaatg aaattttgta gttaaatcat
    15121 ttaatgacaa atatttagat tgacaaaaat tatgatcaac caatataaaa ccagacgccg
    15181 tcctgaaaag gaaatgattt ttggggtaag agccgttatt gaagccattc aatctgggaa
    15241 ggaaatcgaa aaaattctgt tgaggaaaaa tataagtaac gaaacccgaa gacaactttt
    15301 tcagactatt aaagatttgc aaattcccgt tcaatttgta ccggaagaaa aattaaatac
    15361 cattaccaat aaaaatcatc aaggtgtaat tgcttatatt tcacccattt cttatcaatc
    15421 cattgaaaac atcattcctc aaatttacga agaaggaaga atgccttttg tggttgtgct
    15481 agacgaaatt actgacgttc gtaactttgg tgccattgct cgaacttgtg aaggtgggaa
    15541 agttgatgcc attatcattc ctgccagagg tagtgcttcc atcaatgcag atgccattaa
    15601 aacttcggct ggtgcattgt tttccatgcc tatttgtaga gcaaataatc tggaaaatac
    15661 cttgaaattt ttgttggatt cggggctcaa acttgttgcc gcaacggaaa aagcggataa
    15721 aaattatacc gaagtttcct ataaagaacc gatagcacta gtgatgggag cagaagataa
    15781 aggtatccgt tctgaacttt tatctttatg tgaagaaaaa atacgaattc ctatgctggg
    15841 aaaaatcgaa tcattaaatg tttctgttgc ggctggcatt ttgatttatg aagtagttcg
    15901 tcaacgtaat ttgaacagca tttaagtggc atttacataa catttaaatc taatggaaat
    15961 tttatacgaa gataatcatc tcattgccat aaacaaaatc aattccgata ttgtacaggg
    16021 cgacaaaaca ggtgacgagc cactttccga aaaagtaaaa aaatatttga aggaaaaata
    16081 taataagccg ggagcggttt ttttaggcgt tcctcatcgg ctggataggc cagtgagtgg
    16141 cgtcgttttg tttgcaaaaa caagcaaagc tttatctcgc cttaatgtta tgtttcagaa
    16201 ccaagaaatc aaaaaaactt attgggcgat tgtcagacaa aagccaccac accgggaagg
    16261 acaactagaa cattttctgg taagaaatga aaaattaaat agaacttttg cttatgatga
    16321 accggtcaat gacagtaaaa aagcaatttt atcttataaa atccttgcca attctgatta
    16381 ttattattta ctggaagttc atcttaaaac cggaaggcat catcaaatcc gttgtcaatt
    16441 ggcaaaaata ggttcgccga tcaaaggaga cttaaaatat ggctatcccc gttccgacaa
    16501 aaatggtggt ataagtcttc atgccagaaa aatagaattt gttcatcctg tttccaacca
    16561 gctaattcaa attacagcac ctgttcctca agatgataat ttatggcttt attttgaaaa
    16621 tttgcaagaa aataaatgaa tattttatga acaatttaca aaagcctgat tggattagga
    16681 caaaactaac aagtacttcc aatttttcaa ctgttcatca aacgatccag caacatcgac
    16741 ttcataccat ttgcgaaagt ggaaaatgcc caaacttgag tgaatgctgg aacagaggaa
    16801 ctgctacttt tatgatactg ggtgagattt gtacacgttc ttgcaaattt tgcaataccg
    16861 caacaggtcg tcctcttcca ccagatccaa cagaaagtca agccattgct gaagctattt
    16921 attccttaaa actgaaacat gctgtgatta cttctgttga tagagacgat ttgcccgata
    16981 aaggtgcttc cttctgggca aaaactattc atgccattca agcaaaaaat ccccatacaa
    17041 cgatcgaagt acttattccc gactttgacg gacaacacga tttgctggat aaaattattc
    17101 aggaaaagcc agacatcatt tctcacaata tggaaactgt ccgtcgatta acgccattga
    17161 ttcgttcaaa agctcgatat gaaaccagtt tagcagtttt aaattacgtg gcttcgcaag
    17221 gaattattac caaaactgga attatggttg ggttgggtga aacggaagaa gaagtacttg
    17281 aattgatgga tgatgtactc aacattggtt gctctatttt aacgattggt caatatttac
    17341 aaccttctaa aaaaaatatt cctgtatcag cttatatttc tccacaacaa tttgaaaaat
    17401 acaaagaaat aggactcaaa aagggattta aatatgtaga aagtgctcct tttgttcgtt
    17461 cctcttattt tgcagaaaaa catattatat aactcaagta tttttttgat aatcaatgga
    17521 ttttcaatct cgtccaaaaa gataaataag caaaatacct gttaggataa aagtaatctg
    17581 aatgagacct tctttaaagg aagtttttcg atgcatttct ggaatcaagt cggctaaggc
    17641 aatatataaa aagctggctg ctgaaaaagc cagtgcataa ggaatcaatg atttagcaaa
    17701 acttaacgaa aaataagcta taattcccaa cgccaaagcc gttgagcctg aaagggcatt
    17761 ataaaacaaa gcttttgaac ggctcattcc acttcgcaat aaaacgccaa aatcgcccaa
    17821 ctcttgagga acttcatgaa aaacaaccga caatgtcacc acccacccaa aactattgga
    17881 agttaaaaaa gcagaagcaa tgatcactcc atcaatggcg ttgtggaaag catcaccaaa
    17941 taaaaccatt ggagctgcag cagataaatt tctttcgcag gaagcatcat tacaaactcg
    18001 ccacaaaata attttttcca gtacaaagaa aaaaacaagt cctcccaata aaacattcag
    18061 tacgatttca ggtttcgaca cagcgagtgc ttctggaatc attcccagca aagcagaacc
    18121 taacaaagta cctccagcca gataagtcaa aaaacgcgta atccgttgta gcctcttttc
    18181 ctttaataac aaaatcaatc ctgccagtaa tacgctacca acactcccta aaaataaagc
    18241 cagtaaagta aaaataagac tttgcgtcat aaatttttat tttttggtaa ttgagtattg
    18301 taaacgacaa aacttccttt ttctacacca agcaattgtg caatcacatc gcattttgct
    18361 actaataaaa agaaaattct gggatcattt tgatatatta atccttccag atttccctga
    18421 ccttttgaaa taattaaatc tgcctcctgg aaaatttcct gaaattcaga actgcattct
    18481 tttacaattg tagaagctac atcattccca ttggaaatta ctctggcaac tttgtccatt
    18541 tcaacctgat aagcatcttc gagtgtaaca tcattcagtg ctggagcacc acgaacagca
    18601 aaagttacat tgggatgttt cattgtttca ataaacaatt tgtcaaaaac aatttcgcct
    18661 gcattgtcac ccagatacag aattttttta gctattttaa ttcgtttttc taattctgct
    18721 gaatgatcaa tggcaaattg aatagtaaaa gccttttcaa tagtgctatg tacatcaaaa
    18781 tgtgcagtgg gaccataatc cataatgttc cctgcaatag cgagtcgcaa cgccaactga
    18841 aaaggatttt cagacttttc aacaacctct tttaaatcat tatataattc aagggcaatt
    18901 tcattacttt tacgtttttc ttcggcaaag ggatcagaaa gcccactcaa tttcaaaaat
    18961 tctttattca attcacgata aatttcaggc atcagtttac catcactttc acgtaaaatt
    19021 ttctcgtaat gtttttcaaa tttttctcta tcgctttcat ttatgggaaa cttttctatt
    19081 aatctttcaa aattttgtac aaagcaaaat ttgcattgtt cattaattcc actttcttgc
    19141 atttttatta atatattata gtttttttaa ttgtaaatca gttaattact tattagttaa
    19201 taaaggtaat aaattttctt tttcctcttc attggtgtga atgtgtgttt ccttttcatt
    19261 cactaattta aaacaatgtt tgcagcgaaa ccattgctga tcaaattcca cattacctcc
    19321 ttctattaca atcgtttttc cttctaccat tgcttttgct atggttttac gtgcattttc
    19381 gtaaattcgt gttaaagttg gtcttgaaat ttgcatcttt ttagcagctt cagtctgttg
    19441 caatccttca taatccacca aacggagggc ttcatattca tctaaatgta aaattatttt
    19501 tactgattct ttaggaaaaa ttccaaaagg ctgatagccc atcataagtg gtggtgaagc
    19561 tattttacgt tgatgttgag gacgagccat aatctttttc gattttttaa aacaaggcaa
    19621 aattacaaaa aattttgaac ttacgttcat tattttttaa aagaaaaaaa ttctatgtaa
    19681 taaaaataat ttggctttaa ttatagaata ggcttcgtaa aatgtttcta ctacaaactc
    19741 taaattcacg attatcaatt aaatcctata aaccaagact cgcacctcgt aactcgaacg
    19801 ccacacctcg cactttttta gttagcaatt aattgatctt tacttatcaa tgcgaaattg
    19861 aaaattatca cgtaactttg caatcggaag caacaatcat gtaaaagttt acaaatcaaa
    19921 atggtaaatt ctttattaca aaatttatat acacgacaag acatagaaat tactgctcca
    19981 gccggttcgt gggaaagttt aatggcagcc gtcaaggcag gagctgattc ggtttatttc
    20041 ggaattgaaa aattaaacat gagaagcaaa tcgagtaaca actttactac cgaagattta
    20101 cctcatatag taaatatctg caaagaaaat aaagtaaaaa gttatttgac tgttaatacc
    20161 attttatatg acagcgattt ggaattaatg agaaaaatca ttgatcttgc caaaaaagaa
    20221 aacgtagatg ccattattgc ttccgatgta gccgctatga tatatgcaaa tagtatagga
    20281 atggaggtac atttgtccac ccaattaaat atttccaaca tagaagcatt aaaattttat
    20341 gcacaatttg ctgatgttgt ggtgttagca cgcgaactga atttgaatca agtattggaa
    20401 atttatcaaa caattcaaaa agaaaaaatt tgtgggcgaa gtgggagact aattcgtatt
    20461 gaaatgtttt gtcacggagc actttgtatg gcggtatcgg ggaaatgtta cctgagtttg
    20521 cacgaaaaaa atctttctgc taaccgtggg gaatgtaatc aaatttgtcg gcgaggctat
    20581 ttggtaaaag ataaagattc cgaaatagaa ttggaaattg acaatgagta tattatgtca
    20641 ccaaaagatt taaaaactat cagttttata gatcaaatgc tcgaagcagg tgtcagagta
    20701 tttaaaatag aaggaagagc aagaagtgcg gaatatgtaa aaacagtggt atcgtgctat
    20761 aaggaagcca ttgaatcttg tctggatggt acgtacaacg aagaaaaaat aaaaaattgg
    20821 gacaaaaagt taagcagagt attcaatcgt ggtttctgga atggatatta tttgggacaa
    20881 agattgggtg aatggaataa aaattatggc tcggcagcaa ctcataaaaa aatatacatt
    20941 ggcaaagtga cgaactattt cagtaaaatt ggcgttgcag aatttttact ggaaacccaa
    21001 agtttatcag taggtgatga gatattgatt acgggcgaaa caaccggagc ttatgaagat
    21061 actgttacag aaatcagggt agaagatgtt ccagtagaaa aagtagaaaa aggtacctat
    21121 ttctctatga aaaccaacga attggtgcgt cgaaacgaca aacttttcaa aattgttccg
    21181 acaaaacatg ggaaagaaga aagagaatag aattttgaat gaaatatttt ttatgtagct
    21241 atttcctcaa tcaaattta
//
LOCUS       DAOSAS010000012        20529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_1845_length_20529_cov_8.001661, whole genome shotgun sequence.
ACCESSION   DAOSAS010000012 DAOSAS010000000
VERSION     DAOSAS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20529
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1845_length_20529_cov_8.001661"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1214..3739
                     /locus_tag="PLR72_01190"
                     /pseudo
     CDS             1214..3739
                     /locus_tag="PLR72_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
     gene            3855..5876
                     /locus_tag="PLR72_01195"
     CDS             3855..5876
                     /locus_tag="PLR72_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOK35860.1"
                     /translation="MTVMLFSFEVYSQENINISIVDRPEIQVVNKNYVQNRAPLLPLH
                     CIKLPVGDVKPEGWLLEMLYRQKNGLNGHLGEISAWLDKENNAWLGTGTDYSWEEVPY
                     WLKGYGNMAYILGDEKMIEETKMWIEAVFKSQREDGYFGPYIEKNGKPDLWGNMIMLW
                     CLQSYYEYSGDQRVIDLMGRYFKWELALPEDQFLKDYWENSRGGDNLLSVYWLYNLTG
                     DKFLLDLAEKIHRNTANWRQRSTLPNWHNVNIAQSFREPATWYLLSKDSADLISTYNV
                     FHLIRNTFGQVPGGMFGADENARIGYIDPRQATETCGFVEQMASDEWLLRMTGDPFWA
                     EHCENVAFNSFPASMMPDMKALRYLTAPNQVISDSKDHSPGIQNSGPFLAMNPFSSRC
                     CQHNHGQGWPYFIEHLIMASPDNGIIAAIYGACKATVKVADGKTIEIIESTNYPFEEK
                     IKFTIHSSQKVPFPLYLRIPSWADKPFIKLNGKLLNVPLVNGMYLKIEREWITNDEVE
                     LYVPMKLAETIWQVNQQSRSIHYGPLTFSLKIAEDYQIVNSTETAIFDSKWQKNADPL
                     QWPAYEIYPASSWNYGLLINDSLPLENNFMVIKKRWATNNFPFSLENIPIEIRAKGKK
                     LLTWKIDQYGLCSELPIMENRKYGDETHDIILVPMGAARLRISAFPVVK"
     gene            6239..8061
                     /locus_tag="PLR72_01200"
                     /pseudo
     CDS             6239..8061
                     /locus_tag="PLR72_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
     gene            9014..10846
                     /locus_tag="PLR72_01205"
     CDS             9014..10846
                     /locus_tag="PLR72_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK35861.1"
                     /translation="MKKKTFLFLISLVIVCCAFAQTLPDGMVSLLPSGVKANINDTRK
                     AVQYKNLVVAGEKSKGYKAFFAASDNTHGEELWVTDGTVAGTHMVKDINPGTNGSDIN
                     WMDRFNDKVVFAADDGEHGMELWISDGTEVGTYMVKDIHEFGSSNPRGFTQVNETQFI
                     FGAKDFESENYSTRGPQWWLWVSDGTAEGTKMIYDCDTRWPGQDNTSWLSPYCRVGRK
                     VFFKADNKEGTVGEELWVTDGTTEGTVFIKDINTEKIATGTANSAIDNMINFYNEKLF
                     FKAFSIESSNEPWASDGTTEGTYEIYDSNPTFEPSGFPRGGGASDCGSKPYKGKVYFR
                     GFSLETGCELAATNLEQGNFTIWDINRNEPTSNNHSYPDPGVEFDGVYMFCAATGFDP
                     MKPNNYGGELHYTDGETITMQSDMAPGTKSNWVKELTIASGSLYWWNESSELPEYTQK
                     LFRIDNKSQFPVRVTNFSPEGDKIHTLRNLGGDLIFARNDENKSLYVYHYRKEGYDPQ
                     KDADDLDIEFRTRAEIESESSTPTIPIPTKIVLYPNPTNDKFNFEVPGNVTCIKIFDI
                     SGRLVKEETQLISNSVNVSSLVKGIYKVVITSTEGTYVSSLIII"
     gene            10979..14137
                     /locus_tag="PLR72_01210"
     CDS             10979..14137
                     /locus_tag="PLR72_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK35862.1"
                     /translation="MVNNNKHFHIAIFFIAIFSSVFSSVFSYGQNQISISGIVLDSNS
                     KKPLIGVSVLEKGTTNGTTTDLEGKFFLQTTPNATLVFSYVGYISKEVNIDNNTQLTI
                     MLEEKSELLDEIVVVGYGVQKKSDITGSISSLSAKDINNVPVPSPLQALQGKAAGVGI
                     IQNTGSPGSTTTIKIRGTGTINDSDPLYVIDGFIVDNINHLNPNDIANIEILKDAASS
                     AIYGARAANGVVLITTKSGEKGRAKISFDNFVSFSKPWKKIAVMDIEQYALVLDYVNG
                     LTNYSVDGKLYYSKDPVTGEFYYDESKFHRIDTIRNNSPANWWDAITQTGIKQQYNLS
                     VSGGNEKNKYIVSASYYDERGIVKTAGYNRFNTRMNMENQLATWLNLTANIMYTNENR
                     QIIPEGGGSILKKALMQNPMVYTYDSKGYYSSDHPIAIINRNHNKMKRDRIDLNMSLT
                     AQIAKLLTYQFKISDYIVPERWTNFFEVNKLNEDFQMPYDLTTVYVLQNLTNKWEINN
                     LFTFLWNNNIHNLTVLGGQIAEGYNFNYQESIRKGTASNSPNLRYLSSAYTGDKTGGL
                     TRQWTAVGFVGRVNYSLFDRYLFQANFRADASSIFAKKERWGYFPSISFGWKFSSEPF
                     MENVKWLSLGKLRLGWGQLGNNRIEETSRYTLLNTQYNYSYGIGNHILYPGITATTIG
                     NPNIQWEKTETFNIGLDLGFLRNSLTAEIELFNKLTTDMLLRVPVTISAGLDDAPMTN
                     AGSVRNRGVEFVINYKNNINKFRYEIGFNISYIKNKVTSLGTGNEPVYGAWLSESSIL
                     DYVTKTVVGKPIGCFYGYVTDGIFNSYEEIKESAQYEYGKNDWEQTTRPGDFRFKDLN
                     GDGRITADDRTFLGSPLPDFLFGMPLSFSYANFDLDIFFQGQAGNKIFNVMDYYLYNA
                     AEGNLYADIREKHWSGQLQDSRKFFPLNLNASVPDLDPSDEARNFRASDFFIKDGSYV
                     RLKQLRFTYNFNKRINTQLHLSNLALFFDTYNLFTFTRYNGLDPEVGKIVGTESNNLN
                     MGVDHGNYPQSRTFTLGIKIEL"
     gene            14168..15847
                     /locus_tag="PLR72_01215"
     CDS             14168..15847
                     /locus_tag="PLR72_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOK35863.1"
                     /translation="MNKILFKILTLSFSLIFLMSCHDFLDKEILGNSTDENFYDTQYK
                     LQAALNASYDVLQMDIFNECEWRFGEACADDVWGGDEGLASQMGQLVNFRFNTSNEWI
                     QNRYMINYKGIHRVNQVIANAHRVKLSVNDYSSYKAIREIVGQAKFLRALYYFNLVKT
                     YGGVPIRPETETVDKFVIPRSPAEEVYAYIEKDLREAAIMLPARYMGTDAGKASEGAA
                     IALLMKVLMYQSTPGVKSDKWEEIVRLGEFFVEGRKMTFGDILKYDASKEDWENLRKR
                     LWFKPRELNTLTDPYETPNTELPALQNAYSLEYKDYYGKNISYCDQFFQVGEFCKGSI
                     FEIVFKESADGSTGDINEGTPIYDNHYSSSPQIWTTADIIQELFANDPRRAFTIGHQQ
                     YAPDGDLCQCGPGRYLSLKWYTPLKDRPQYAGDNGKNRRLIRYVEVILMYAEALNECH
                     EGARALEQLNKVKAQANTINNSGVLYTGGGYGLMRDQIWKERRIELCFEWDRFFDLVR
                     QKRAAEIIQTYGSKRANKRGYYFRKGVNELFPIPQSEIDISNGIVQQNPGY"
     gene            15879..16856
                     /locus_tag="PLR72_01220"
     CDS             15879..16856
                     /locus_tag="PLR72_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35864.1"
                     /translation="MKTIIKFFLFVCFSIFITSCERENVLFPYAAMNVNKNEFTINES
                     MIINFTGTADQVVIYTGDDMHNYEFRDQSNTGFVVNKGLFTYSYALPGIYKVVCVAST
                     YNDRAVNLKRDTCSFIVKVIDDQTEIEKISCPQVLYDEVFADKLENDEWLMKLPRKVK
                     YGNATPSITLSQKLRFYIQSNLTKIFINEKEFSSTEKYDLSNPLNILVQSNFGTVRPY
                     KLYTLYYPEFVTFKLLNVNGTLVRNEFDYSTFEMQIILPMGTDVSNLIPEFTTYSSTD
                     KVYIENKEQISGVSRVDFTQNITYTLVSSLLEDSNIKVMSTVNIKIIYQ"
     gene            16882..18846
                     /locus_tag="PLR72_01225"
     CDS             16882..18846
                     /locus_tag="PLR72_01225"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOK35865.1"
                     /translation="MKKISIIIIIAIAFTISSFTQELTPYQGSRIFWDINSQTTVFQP
                     GNYARMIELQDGRLLAVAEALGGISITFSSNKGNTWTSPRLITSPPNKVFYAVPDVIQ
                     LSDGIILIGFNPRPHAPYSPDRLFGIRVVRSTDNGETWSDPYFIFDAHYTFDNGCWEP
                     AFLELPSGEVQCYFANESEYTNSNEQNISMCRSFDKGITWSTPIIVSFRQGYRDGMPV
                     PLLLKDQSEIVVIIEDNGWPGRGNFVATTVRTTLEDNWTKGYVDGNSSKRSMIFQIPP
                     PTNIVSAAPYIRKFPWGETIASYQSNENRISNDLQYFDMYVVIGDERARNFKAKSAPF
                     SLGNDKHAIWNSISVIDTGIIVALGSIGPPYKSNDVVMMKGYPLRQAKANYGVINVDG
                     TKSLDEKWTTSNASQLYFGHITKNKATVDFLYDEKYLYLTSQIIDRNIINTGLDNDGI
                     RFMIDADNISGSMPETGMYSFFFDTNGTVKFQRGNHGIWTTDNNVSEILYAVDIKSFY
                     YNIEAAIPWSLIGKSAPPFGERMAIAIEIVNKEEYTLTKEGIADVDNNASWTWLEFQL
                     IPRQSNKNICVNEDNSVIKTFVNNNILYINSPVMIKELSLFSLEGKLIDKKKEVGLNF
                     HIPIPIPFYKGIVLMFSLENGEIINKKILF"
     gene            19111..20088
                     /locus_tag="PLR72_01230"
     CDS             19111..20088
                     /locus_tag="PLR72_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOK35866.1"
                     /translation="MYKFEFFFNLLLFIIFFKSNYIKGENFVTTVSIEKKVPLADPYI
                     LLYDGMYYAYGTGAEDGIEVLTSSDLKSWKRTAGTPTGLALHKDDSYGEKWFWAPEVY
                     FVNGKFYMYYSAEEHICVAISDSPLGPFRQTEQKPMLEEEKAIDNSLFTDDNGKAYLF
                     FVRFNDGNNVWVAELENDLMTLKKETMHHCISVSQSWEEVWPRVNEGPFVIKHNGIYY
                     MTYSANSYESPFYGIGCATATNIMREWTKYPDNPLLQNPGDLVGVGHNSLFWDKDGNL
                     RIVFHAHHDKNTIHPRNMYISNAGFKNVNGIDKFYIDPNYQTPVLEVNK"
     gene            20229..>20529
                     /locus_tag="PLR72_01235"
     CDS             20229..>20529
                     /locus_tag="PLR72_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOK35867.1"
                     /translation="MFFVVFFLILSFTLPVWSLPSYQFKHYDINKGLSQNTVLSILQD
                     KQGFMWFGTKHGLNRFDGKSFKVFKFFPNSEVKDNVFRCILQDKDEKLWLGTDEGV"
BASE COUNT         6999 a         2989 c         3879 g         6662 t
ORIGIN      
        1 gactttcatc ttgtcccttc ttttttatct tatcttttgt cggtcagtag tgaaaattta
       61 tataatagta ctcctaaaaa atagagttag tgattaaatt aaaaaatgac taaatttggt
      121 ggtaattatg aaataaacaa ggagaaaatt ttcagcagat tttgaagcta aagtagcaat
      181 taaggcgatc acggagtgag aatcacttgg tgaattatca aagtgttttt taggtgcact
      241 cccacacaat caacaaatag aaacaggagt ttgcaaaacg aagtagtgaa ttatttgaca
      301 aaaagacagg gtctaaaagg aatgttgata tagaaaaact aaagacaagc attatacagc
      361 ctacgggatc cagtggtcta aaaggaatgt tgatatagaa aaaccatcct ccaaaatagg
      421 tgagttggac atggaaaatg actttttaaa aaacttaaag aaaattgaac tgtgacagaa
      481 cataggaaac ttgtggacaa aaattatgac ctaaaagaaa tctgagcaag ttggtggtga
      541 gttaatgtat ttggctatac tcgttaaagg gattgaagat agagtactct aatcagggat
      601 ggactattga tatttcctat attcccatga agaagggttt ttttgtattt aacggccata
      661 attgatcttt acggtcaata atagtcggat gggatgtctc acactcttga aggagaggtt
      721 actttgtccg tcatacaaga ggctgtaaaa gaacatagga aactggagat tgtcaattcc
      781 gatccaaaaa ggaagccagt ttatttgtga aaagtggata agctacctga aagcgaaaat
      841 atcaagatca gcatggatgg taagaccaga gcgatcaaca atatctttat ataatacctc
      901 tggaaattat tgaagtatgg ttatgtatat ttgctcctgc aaatgatggt ttggaattgt
      961 atcaaaagaa gaaaattctc taaagttata ctgtcatgca gcacaaaaat gagggaagat
     1021 gatttctcaa aaaagatatc atcattactg gtgaaaccat agaaattaca ggttacgaag
     1081 gatcatacag aaacgaaaaa tttggcagat cgatattctg aaaaagttga gaaaatgaac
     1141 aatttataga agaagtaagc tgattcattg aatattaaat attaaacttt tataaattaa
     1201 tagagaataa attatgagaa ggaacaattt tttattttta ctgacttttt tacaattacc
     1261 tttttacctg ttttgtgaaa caaaagatca atcaatttcg gaactacgag gaccagcata
     1321 tccgttagta acagtagatc cctatttcag cgcatggtct tttgctgata atctgtatga
     1381 tgatccggta agacattgga caggaaaaga atttggattt ataggagcca tcagggtgga
     1441 cggaaaaacg tatcggtttc tgggtacgga aaatctaccg ctgaaacctc ttgccccgac
     1501 ttcttttcaa gaaaaatggg aagctgttta tacttttaag aaacccgtat cggaaaattg
     1561 gacatcactt gaatttgacg atacggcttg ggagaaaggt aaagctgcat tcgggacaaa
     1621 aggggaaccg aatttatcga ccctttggga aactcaagat atctgggtga ggagaacctt
     1681 taaccttaac cacgatacaa agaataaaac cgtgtttctc aattattcgc acgatgatat
     1741 ttttgaactg tatattaatg gcaaaaaaat tatcgatacg ggatattcat ggaaatataa
     1801 tgtgcttttg gaacttcccg atgaagtaaa agctacactt aaaaaaggaa aaaacgtgat
     1861 cgctgcccat tgtcataatc gaacaggggg tggttatgtg gattttggtt tatttgaaaa
     1921 agaaacacaa aaagagtcgt tcgttacaac aactgttcaa aagtcagcaa gtgtattgcc
     1981 aacccaaaca ttttatactt ttgaatgcgg tcctatagaa ttggaattga ttttttccgc
     2041 ccctttgttt ttggacgatt tggatttaat gtctcgccct gtcaattatg ttacctatcg
     2101 tgtgaaggct tccgataata aacctcataa cgtccagatt tattttgaaa caacacccca
     2161 atgggcggta aatacggaca accaattagt agatttcgaa aaaattgaaa aagatgacct
     2221 gatttatttg aaaactggaa ctaaagagca gcccgtatta caaaagaagg gagatgattt
     2281 acgaatcgac tggggatatt tttatatgac tggaaaatcc gaaaaatctt cggttatgaa
     2341 ttttggtaat tattggaaaa tgaagaaaga ttttcaattt aaaggaaaaa taactccacg
     2401 aatttcgact ccattatctt caaatatgac ggaggaaatg actgcattat cttacagtaa
     2461 agatttgggg aaagtttctt ccaattttgt cgaaggctac attatgcttg gctatgatga
     2521 tctttattcc attcagtatt ttcacgataa tttgctgcca tattggaaaa aggacgggca
     2581 agtggatatt tatcgtgtct ttaaggaagc tgaagagaat tatgaagcta taatgaaacg
     2641 ttgtaatcgt tttaacaagg agctgatgag ggatgcaaat aaatgcggcg gaaagaaata
     2701 tgccgaactt tgtgccttag cttatcgaca agctattgct gcctataaat tggtaaaaga
     2761 taaaaacaac aatttattgt tcttttcaaa ggaaaatttc agcaacggct caatcggcac
     2821 ggtagacata acttatcctt catcgccact ttttttgatt tacaacgctg aattgctcaa
     2881 gggaatgata aatccgatat tttattacag cgagagtgga aaatgaacaa aaccattcgc
     2941 agctcatgat gtgggaactt atccacttgc taatggacaa gcctatggcg acgatatgcc
     3001 aattgaagaa tcaggaaaca tgcttattct taccgctgcc attgccgaga tggaacaaaa
     3061 tgccaactat gctgacaaac attgggaagt attgacgact tgggcagatt atctggtgaa
     3121 ggaaggatta gatcccccca atcaactttg tactgacgat ttcgccggac attttgcaca
     3181 taatgcaaat ctttccataa aagcaattat ggggattgcc ggctatggga aattagcccg
     3241 aatgctcggg aaagaggatg tagcggaaaa ttatttgcaa attgccagag aaatggctga
     3301 aaaatgggaa ataatggcaa atgacggaga tcattataaa cttacttttg accgacctgg
     3361 gtcgtggagc caaaaatata atcttgtttg ggacaggcta ttgggattca atatcttcga
     3421 tacgaaaatt gccgaaaagg agattgccta ctatcttacc aaacaaaatc ctttcggctt
     3481 gcctttggat aatcgaaaga cctacacaaa atctgactgg attatgtggt cagcgatttt
     3541 ggcagaaaat actaatgatt ttaacgcatt ggtggatcgg gtatataaat atgcgaatga
     3601 aacgccaacg cgtattccgt tgagtgattg gcacgaaacc attgacggta ccagtgtggg
     3661 attcagggca cgttcagtag taggaggata ctttatgaag atgctggagg ataaaattca
     3721 atccattcaa tataagtaaa ttctttttat atttgttttc tccaaataat gggttttttt
     3781 aagaaaaaag tagaaaaggt atttaatagt aaattatgag aacaataaaa ggtgtatttg
     3841 tgaaacctat tgtaatgact gtgatgttgt tctcttttga agtatattct caagagaaca
     3901 taaatattag cattgtagat cgacctgaga ttcaagtcgt caataaaaat tatgtgcaaa
     3961 accgagcacc actgcttcca ttgcattgca taaaattgcc cgtaggtgac gtgaaaccgg
     4021 agggttggct tttggaaatg ctttatcgtc aaaaaaatgg actaaatggt catttgggcg
     4081 aaataagcgc ttggcttgat aaagaaaata atgcatggct ggggacggga actgattaca
     4141 gttgggagga agtaccctat tggttgaaag gttatggtaa tatggcgtat attttgggtg
     4201 atgaaaaaat gattgaggaa acaaaaatgt ggatcgaagc cgttttcaaa agtcaacgtg
     4261 aagatggtta tttcggtccg tatattgaga aaaacgggaa accggatctg tggggaaaca
     4321 tgatcatgct ttggtgttta caatcttatt atgaatattc aggagatcaa cgtgtgattg
     4381 atttgatggg acgttatttt aaatgggagt tggctttacc ggaggatcag tttttgaaag
     4441 attactggga aaatagtcgt ggaggagata atttgttaag cgtatattgg ctttataacc
     4501 taacgggtga taaatttttg ttggatttgg ctgaaaagat tcataggaat acggctaatt
     4561 ggcgtcaacg ttcaacactt ccaaattggc acaacgtgaa tattgcgcag agttttcgtg
     4621 agcctgcaac atggtacttg ctttcaaaag attcggcaga cttaatttcc acttataatg
     4681 tgtttcattt gattcgaaat acatttggcc aagttcctgg cggtatgttt ggagctgatg
     4741 aaaatgcaag aataggttat atcgatccac gtcaggcaac tgaaacttgt ggttttgttg
     4801 aacaaatggc ttctgatgaa tggttactaa gaatgactgg tgatcctttc tgggcggagc
     4861 attgtgaaaa tgttgcattc aactcatttc ctgcttctat gatgcctgac atgaaagctc
     4921 tgcgttatct tacagctcct aatcaggtaa taagtgattc taaagatcat agtccgggta
     4981 ttcaaaacag cggtccattt ctcgcaatga atccttttag cagtcgttgc tgtcagcata
     5041 atcatgggca gggatggcct tattttatcg aacatctgat tatggcatcg cctgataacg
     5101 gtattattgc ggctatttat ggtgcctgca aggcaacagt gaaagtagcg gatggtaaaa
     5161 cgatagaaat aatagaatcg acgaattatc cttttgagga aaaaattaaa tttaccattc
     5221 actcctctca aaaagtacca tttcctttat atttaagaat tccttcatgg gctgacaaac
     5281 cctttattaa gttgaatgga aaattgttga atgttccttt agtaaatggt atgtatctca
     5341 aaattgaaag ggaatggata acaaatgatg aagttgaatt atatgtgccg atgaagcttg
     5401 ctgaaactat atggcaggtt aatcagcaaa gtcgaagtat tcattatggt ccattgactt
     5461 tttcattaaa aattgcagaa gattatcaga tcgtaaacag tacggaaact gctatttttg
     5521 attcgaaatg gcaaaaaaat gcagatcctc ttcaatggcc agcttatgaa atttatcctg
     5581 caagttcatg gaattatggg ctgcttatca atgacagttt acctttggaa aataatttta
     5641 tggtaattaa gaaaagatgg gcaaccaata attttccttt ttctcttgaa aatattccaa
     5701 tagagattag ggctaaaggg aaaaaacttt taacatggaa aattgatcaa tatggcttgt
     5761 gtagcgagtt gccaataatg gaaaatcgaa aatatggaga tgaaacgcat gatattattt
     5821 tagttcctat gggagctgca cggttaagaa tatcggcctt ccctgtcgta aaataaactt
     5881 taaaaaatat agaataaaaa gattccgtaa atttttgttt ttaagcgtat ttttattcgc
     5941 gagttgggca cttattgctc agcctgtaaa aacttccaat ggaattatta tctcttctga
     6001 tgggaagtcc attgaattat cagttgttgc aaatgcagct ttttgcctga gcttcagtac
     6061 ttcgggcaat cctgttgaaa tcaagagtat ttttttagat gaaaataagg ataattcaaa
     6121 aaatcaattt tcaattgttt cccaaaaatc ggtcattgga attaaaaccg atttcggaaa
     6181 actattgttt gacacccaga aacaaacatg gacattgtca aatgtaaaag gaaaagtctt
     6241 gatttcgaac gctacctttt catttggaga ttccattcag aaaatcaatt attctttccc
     6301 ccaaaagagc ttattttatg gatctggtaa tttttcaaca aagaatttac tgaaatcgca
     6361 gtcggcaggg aagcagggca acggaacggc tgatgttcct tacttctgga gtaatgttgg
     6421 cttttgcagt cttggaatat ctggaaacga taatcatccg gcaacgtgga aagccgttga
     6481 aaattcctca gtagaatgga ctttctcagg aaaaacggga aatttatatc tttggccagc
     6541 ttccaatctt tatgaagcca ccaataatct tatcgctatt accgggaaag ctaaaattcc
     6601 tccacgctgg gcttttggct ttttgcaatc gcaatgggga tgggctgatc gtgcttacat
     6661 cgagaatgcc atcgataatt ttcgttcacg taaacttccg gtcgatgcct ttatttacga
     6721 ttttgaacgg tacaccactt tccccgatta tttcgtgaaa gaaaatggta taaaagggtt
     6781 ttctgatttc tcttttaatg aaaaattgtt ccccgaacca caaaagcaaa ttgcagaata
     6841 taaaaagaaa ggcataaaat ttattggtat tagaaagcct cgtcttgctg attcaattag
     6901 gctagtttta gctcgaaaaa atggctggtt attaaaatca gcatataata atcgtgagct
     6961 ggactttagt aatcctgatt tagttagatg gtataaagag caaacccgcc cactcttgga
     7021 agccggtgtt gatgcatggt ggaatgatga aggagaatcc tattatacct gttattattt
     7081 ttggaataaa gcagaattcg agttacgcaa cgaggtattt cccaatactc gatttttctc
     7141 gattaaccga tctttttcac ccgggaatca gcgatttggt tattgtacat ggaatggaga
     7201 tataaaatcc acgtgggaag ttttacagaa aacaccggcc gatttattaa actacagcct
     7261 ttcgggcatg ttttacggtt cttgtgatat aggtggtttt ggagaaaaag atccgtcaaa
     7321 agaattagta gttcgatggt atgaagccgg agttttttac cagtaatgag agctcattcg
     7381 aatttgttta ccacgcctcg tttcccttgg ttgtggggcg aagaggccga agctgccatt
     7441 cgcaaatcgc ttaacttgcg ataccaactt attccgtatt tttacagtgt agctcacgaa
     7501 gcatatcaaa agaatagacc cattatgcgg ccattgatca tggaattccc ccacgacact
     7561 gcacttttga atttgacaga cgaatggctt gtgggtaaag atttactcgc tgctccgatt
     7621 ttaaacgaag gcggaaatcg aagtgtgtat cttccagacg ataaatggta cgactttcaa
     7681 accaatcaat taattaccgg tccttgtaaa cttgcagttt ccaaagctct cgacgaaatt
     7741 ccggtatatg taagagccgg agccatactt cctattggcc caattgtgca gcataccgaa
     7801 caggacacca cttcggtcat tgaagttcat atttatccag gaaaaaatgg ttcattctgc
     7861 ttaacggaag ttgacggaag atcatacgac tatgtttccg gaaaagtcag gaatgctttt
     7921 tttgaatgga acgatgctac aaaaacgctc agttggaaaa ctacaggcga ttttgtggga
     7981 aaatacaaaa gtatcaaagc tgttttgttc gatgtggcta aaactgcttc gttaagtgaa
     8041 aagggaaaaa ttaagtttta atcgctttca ctaaatgaat gatttttttt ctaatggtgt
     8101 aaataatttc tctgaaattt tcactgaaat gtattaaagt tttttatatc caaatttctc
     8161 aatatttctt tattagagta ctattatcga ttaactatat ttttggattg ctatcgtccg
     8221 gtgagccgaa aattaaccag tttcgagttt acgaatttcg aattttgaac taagaaatgg
     8281 acacagtttt gaaagcaagt ttaaatgaaa acaaaatcaa atttatttga attctgttga
     8341 acgtaactgc ttcaacgaag ttactttttg cttacttttt atgtcaagat aattagacga
     8401 ggtaaaatcg ccaaaacaat taaaaaaaaa ttaaacactt tctgaggtaa aatagtaagt
     8461 ggagttggga caaaacccct gatacaaaat actattcagt aaattttaaa caatttttat
     8521 cttgtgttta tagtattttg tttataaaat caagtaagaa tttcttacgt tcaattttga
     8581 aaaataaatt taattctgct aacatttttc tcaatacaat atattatagt tcagataatt
     8641 ataacttatt tttctagtga ccgccattag aaattaaatt ctaatgacaa atttggatta
     8701 aatttatctt ctgagtaaac ttttaatgag tctatttatt caaagtttaa attctttttg
     8761 attttgtaat aagctttctc ccaataaatt tttttcaaaa tattaaaaag ttgaaaatgc
     8821 tttttaattg atagcttaat aattacttct tgatttttct ttccgcttat tattcctgtt
     8881 ttaacataat gaaaatgatt attgatatta tttttttatt ttttgataca aaaattatac
     8941 attattttta tttttggttg tacttttatg ctacaattta taaacaaatt attaacattt
     9001 aatttttaac aacatgaaaa agaaaacttt tttatttttg atttctctgg taatagtttg
     9061 ctgtgctttt gcacaaacat taccagatgg tatggtaagt cttttgcctt ctggagtaaa
     9121 agcaaatatt aatgatactc gtaaagccgt acaatacaaa aatttggttg tagcgggaga
     9181 aaaatcaaaa ggatataaag ccttttttgc tgcttcagat aacactcatg gtgaagaatt
     9241 atgggtaaca gatggaacag tagccggtac tcatatggta aaagatatta atccaggaac
     9301 aaatgggtcg gatattaact ggatggatag atttaatgac aaagttgttt ttgctgccga
     9361 tgatggagaa catggtatgg aattatggat ctcagatgga acagaagttg gtacttatat
     9421 ggtaaaagac attcatgaat ttggttcatc taatccacga ggatttactc aagtaaatga
     9481 aacacaattt atttttggtg ctaaagattt tgaaagtgaa aattatagta ctcgtggtcc
     9541 gcaatggtgg ttatgggtaa gtgatggtac tgctgaagga acaaaaatga tatatgattg
     9601 tgatacaaga tggccaggac aagataacac aagttggtta agtccttatt gccgtgtagg
     9661 tcgtaaagta ttctttaaag ctgataacaa agaaggcaca gtgggggaag aactttgggt
     9721 tacagatggc acaacagaag gcactgtatt tatcaaagat attaatacag aaaaaattgc
     9781 gacaggtaca gctaattcag caattgataa tatgattaat ttttataatg aaaaattatt
     9841 ctttaaagct ttctctatag aaagtagtaa tgagccttgg gctagtgatg gaaccacaga
     9901 aggtacttat gaaatatatg attctaatcc aacttttgaa ccttctggtt ttccacgggg
     9961 cggtggtgca tctgactgtg gtagcaaacc atacaaagga aaagtatatt ttcgtggttt
    10021 ttctttggaa actggatgtg agcttgcagc aactaattta gaacagggca attttactat
    10081 ttgggatatt aatcgaaatg aaccaaccag taacaatcat agttatcctg atccaggtgt
    10141 tgaatttgat ggagtttaca tgttttgtgc tgcaacgggt tttgatccta tgaaaccaaa
    10201 taattatggt ggagaacttc attatacaga tggagaaaca attaccatgc aatcagatat
    10261 ggcacctgga acaaaaagta attgggttaa agaattaact atagcatcag gtagtttata
    10321 ttggtggaat gaaagctctg aactacctga atatacccaa aaactattta gaattgataa
    10381 taaatcccaa tttcctgtac gtgttaccaa ctttagtccg gagggagata aaattcatac
    10441 tttgcgtaac ttaggcggag atcttatttt tgctagaaat gacgaaaaca aaagtttgta
    10501 tgtttatcat tatcgtaagg aaggatatga tccccaaaaa gatgccgatg atttagatat
    10561 tgaatttaga actcgagcag aaattgaaag tgaaagtagt actccaacta ttccaatacc
    10621 aactaaaata gttctttatc ctaatcctac caatgataaa tttaattttg aggtaccggg
    10681 aaatgttaca tgcataaaga tttttgatat tagtggaaga ttagtaaaag aagaaactca
    10741 attaataagt aattcagtaa atgtatcttc ccttgtaaaa ggtatttata aagtggttat
    10801 tacaagcaca gaaggtactt atgtgtctag tttaatcatt atataaatac tatttttatg
    10861 gtaatggata tttcataaac gattattttt agggaatatc cattactatt ttttattcgg
    10921 gcaattttta ttttaagatt atattttatc tttttatctt cttaaattaa attagtaaat
    10981 ggttaataat aacaaacatt ttcatatagc aatttttttt attgctatat tttcttctgt
    11041 attttcttct gtattttctt atgggcaaaa ccaaatttca atatcaggta ttgttttaga
    11101 cagcaacagc aaaaaaccac ttattggtgt ttctgtattg gaaaaaggaa caacaaatgg
    11161 cacaacaact gatttggaag gaaaattctt tttgcaaaca actcccaatg ctactcttgt
    11221 ttttagttat gtcggttata taagtaaaga agttaatatt gacaataata ctcagttgac
    11281 tataatgtta gaggaaaaaa gcgaacttct ggacgaaatt gtagttgtgg gttatggggt
    11341 acagaaaaaa agtgatatta caggatcaat ttcttctctt tcagcaaaag atatcaataa
    11401 tgtgccagta ccatcacctt tacaagcctt acaaggtaaa gcagcaggtg taggtattat
    11461 acaaaatact ggatctcctg gaagtactac tacgattaaa atacgaggta ccggtactat
    11521 taatgacagt gatcctctat atgtaataga tggctttatt gttgacaata taaaccattt
    11581 gaatccaaat gacattgcta atattgaaat tcttaaagat gctgcttcaa gtgctatata
    11641 tggtgcacga gcagcaaatg gagttgttct gataacaaca aaaagtggag aaaaaggtcg
    11701 tgcaaaaatt tcttttgaca attttgttag tttctctaaa ccatggaaaa aaatagctgt
    11761 aatggatata gaacagtatg ctcttgtatt ggattatgtt aatggcttga caaattattc
    11821 agttgatggc aaattatatt attctaaaga tcctgtcaca ggtgagtttt attatgatga
    11881 gagtaaattt catcgaattg ataccattag aaataattca cccgcaaatt ggtgggatgc
    11941 aatcacacaa actgggatta aacaacaata taacttatcg gtctctgggg gcaatgagaa
    12001 aaataaatat attgttagcg ccagttatta tgatgaaaga ggtattgtta aaaccgcggg
    12061 atataatcgt ttcaatacac gtatgaatat ggagaaccaa ttagcaacat ggttaaattt
    12121 aactgcgaac attatgtata ctaatgaaaa tcgtcaaatt attcctgaag ggggaggaag
    12181 tattctaaaa aaagctttaa tgcaaaaccc aatggtttat acctacgatt cgaaaggtta
    12241 ttattcaagt gatcacccta tagctattat aaatagaaat cacaataaaa tgaaacgtga
    12301 tcgtattgac ttaaatatga gtcttacagc tcaaattgca aaattattaa cttatcagtt
    12361 taaaatatca gattatattg ttcccgaaag gtggacaaat ttctttgaag tcaataaatt
    12421 aaatgaggat ttccaaatgc catatgatct tactacagtt tatgtacttc aaaatttgac
    12481 taataaatgg gaaatcaaca atttgtttac atttttatgg aataataata ttcataattt
    12541 aacagtttta ggaggtcaga tagcagaagg ttataatttc aattatcaag aatcaattcg
    12601 aaaaggaact gccagtaatt cacctaattt aaggtattta tcaagtgctt atacaggaga
    12661 taaaacagga ggacttactc gtcaatggac agcagtaggt tttgttggac gagtaaatta
    12721 tagcttattc gatcgttatt tatttcaagc aaattttcga gctgatgctt cgtctatttt
    12781 tgcaaaaaaa gaaagatggg gatacttccc ttctatttct ttcggttgga aattttcgtc
    12841 cgaacctttt atggaaaatg taaaatggct tagtttagga aaacttcgtc ttgggtgggg
    12901 acaattaggt aataatagga ttgaggaaac ctctcgctat acgcttctaa atacacaata
    12961 taattattct tacggtattg gtaaccatat tctttatcca ggaattactg ctaccactat
    13021 aggaaatcca aatattcaat gggaaaaaac tgagactttt aatatcggtc ttgatttggg
    13081 ttttttaaga aatagtttaa cagctgaaat tgaattattt aataaattaa ctacagacat
    13141 gctactacgt gtcccagtaa caatctctgc aggacttgat gatgccccaa tgaccaatgc
    13201 tggttctgtt cgtaatcgag gggtagaatt tgttataaat tataaaaata acataaataa
    13261 atttagatat gagattggat ttaatatttc ttatattaaa aacaaagtaa ctagtttagg
    13321 tacaggtaat gaaccagttt acggagcatg gttgagtgag tccagcattc ttgattatgt
    13381 aactaaaaca gttgtaggta aacctattgg ttgcttttat ggttatgtaa cggatggaat
    13441 ttttaattca tatgaagaaa taaaagaaag tgcccaatat gaatatggca aaaatgattg
    13501 ggaacaaaca acacgtccag gagattttcg ttttaaagat ttgaatggag atggacgtat
    13561 tactgctgat gatcgaactt ttttaggctc acctttacct gattttttat ttggaatgcc
    13621 tttgtcattt agttatgcta attttgattt agatatattt tttcaaggac aagctgggaa
    13681 taaaattttt aatgtaatgg attactactt gtataatgca gcagaaggta acctttatgc
    13741 tgatatacgc gaaaaacatt ggtcaggtca attacaggat agtagaaaat ttttcccatt
    13801 aaacttaaat gcttctgttc cagatcttga tccttcagac gaagctcgca atttcagagc
    13861 ttctgatttc tttattaaag atggttcata tgttcgtctc aaacaattgc ggtttactta
    13921 taattttaat aaaagaataa atactcaatt gcatttgtca aatttagccc tattttttga
    13981 tacatataat ttatttacgt ttacccgata caacggttta gatcctgaag tagggaaaat
    14041 agtaggaacg gaaagcaata atttaaatat gggagtggat catggaaatt atccacagtc
    14101 gcgtacattt actttaggaa ttaaaataga attgtaatca caaaaataat cataaaaaat
    14161 atcaactatg aataaaatct tattcaaaat attaacatta agtttcagtt taatatttct
    14221 aatgagttgt catgattttt tggataaaga aattcttggg aattcaactg atgaaaattt
    14281 ttacgatact caatataaat tgcaagccgc tttaaatgca agttatgatg tattgcagat
    14341 ggatattttt aatgaatgcg aatggcgttt tggtgaagct tgtgccgatg atgtttgggg
    14401 cggcgatgag ggattagcta gtcaaatggg acaattggtt aattttagat tcaacacttc
    14461 taatgaatgg attcaaaata ggtatatgat taattataaa gggatacatc gagttaatca
    14521 agtcatcgct aatgcccata gagtgaagtt atccgttaat gattattctt cttataaagc
    14581 tatacgagaa atagtaggtc aagctaaatt tttaagagct ttatattatt ttaatcttgt
    14641 caaaacctat ggtggtgtac ctattagacc agaaacagaa acagttgata agtttgtaat
    14701 tcctcgtagt cctgcagagg aagtatacgc ttatattgaa aaagatttaa gagaagcagc
    14761 tattatgtta ccagcacgtt atatgggtac cgatgctggg aaagcaagtg agggtgctgc
    14821 tattgctcta ttaatgaaag ttcttatgta tcaatcaact ccaggagtaa aatctgataa
    14881 atgggaagaa atcgtacgtt taggtgaatt ttttgttgaa ggtagaaaaa tgacctttgg
    14941 ggatatactc aaatatgatg catcaaaaga agattgggaa aatctccgta aacgtctttg
    15001 gtttaaacct agagaactaa atactttaac agacccatac gaaactccaa atacagaatt
    15061 acccgcatta caaaatgcat attcacttga atataaagat tattatggaa aaaatataag
    15121 ttattgtgac caattttttc aggtaggtga attttgtaaa ggctctattt ttgagatagt
    15181 atttaaagaa tcagctgatg gttctacagg agacataaat gaaggaaccc ctatttatga
    15241 taatcattat tcaagctcac cccagatctg gacaacggct gatataattc aagagttgtt
    15301 tgcaaatgac cctcgtcgag ctttcaccat cggtcaccag caatatgctc ctgatggtga
    15361 tttatgtcag tgtgggccag gacgttatct atctttgaaa tggtatactc ccctcaaaga
    15421 tcgtccgcaa tatgcaggag ataatgggaa aaataggcgt ttaattcgat atgttgaagt
    15481 cattttaatg tacgctgaag cattgaatga atgtcatgag ggtgcaagag cattagaaca
    15541 acttaacaaa gtcaaagctc aagctaatac tataaataac agtggagtct tatatacagg
    15601 tggtggctat ggcttaatgc gtgatcagat ttggaaagaa cgtcgtatag aactttgctt
    15661 tgaatgggat cgtttcttcg atttagttcg tcaaaaaagg gcagcagaaa ttatccaaac
    15721 ttatggttca aaacgcgcta ataagcgagg ttattatttt cgcaaaggag taaacgaatt
    15781 atttccaatt cctcaaagtg aaatagatat ttccaatgga attgtacaac aaaatccagg
    15841 ttattaatat atgattaatt agacataaaa taaaaaagat gaaaactatt attaaatttt
    15901 tcttatttgt ttgtttttca atatttatta cttcttgtga aagggaaaat gttctttttc
    15961 cttatgcagc aatgaatgtt aataaaaatg aatttacaat aaatgaatca atgattatta
    16021 actttacggg cactgcagat caagttgtca tttacacagg cgatgatatg cataattatg
    16081 aatttcgaga tcaaagtaat actggttttg tagttaataa aggtttattt acttattcat
    16141 atgctttacc cggcatatat aaagttgtat gtgtagcttc tacgtataac gatcgcgcag
    16201 ttaatttaaa acgtgacaca tgttcattta tagttaaagt aattgatgat caaacagaaa
    16261 tagaaaaaat ttcttgtcct caagtattat atgatgaagt atttgctgat aaattagaga
    16321 atgatgaatg gttaatgaaa ttaccacgaa aggtaaaata tggtaatgca acaccatcaa
    16381 taacactttc tcaaaaatta cgtttttata ttcaatcaaa tttaactaaa atttttatta
    16441 atgaaaaaga attctcgagt acagaaaaat atgatctgtc aaatccactt aatattttag
    16501 ttcagtctaa ttttggaaca gtacgaccat ataaattgta tactctttat tatccggaat
    16561 ttgttacttt taaattgctt aatgtcaatg gaacattggt acgtaatgaa tttgattata
    16621 gcacatttga aatgcaaata attttgccta tgggtactga tgtcagtaat cttatacctg
    16681 aatttacaac atattcgtcg acagacaaag tttatattga aaataaagaa caaatttcgg
    16741 gtgtttcaag ggtagatttt actcaaaata ttacttatac tttagtatca agtttattag
    16801 aggattctaa cattaaagtg atgtctacag taaatataaa aatcatttat caataatcaa
    16861 ttaataattt aataaaattt aatgaagaaa atatcaataa taataataat agcaatagcc
    16921 tttaccatat cctcttttac tcaagagtta actccatatc aaggatcacg aatattttgg
    16981 gatataaata gtcaaactac cgtatttcaa ccaggaaatt atgcacgtat gattgaactt
    17041 caagatggac gattactagc agtagcagag gcattgggag gtattagtat aacatttagc
    17101 tctaataaag gaaatacatg gacctctcct cgtcttataa catctccacc taataaagtg
    17161 ttttatgctg ttcctgatgt aatacaatta tcggatggca ttattttaat aggctttaat
    17221 ccacgtccac atgctcctta ttctcctgac cgtctttttg gtatccgtgt tgtgcgtagt
    17281 actgataatg gggaaacatg gagtgatccc tattttatat ttgatgcaca ctacacattt
    17341 gataatggat gttgggaacc agcatttctc gaattacctt caggagaagt acaatgttat
    17401 tttgctaatg aaagtgaata tacaaatagt aatgaacaaa acatttctat gtgtcgatct
    17461 tttgacaaag gaataacgtg gagcactcca ataattgtaa gtttccgaca aggttacaga
    17521 gatggtatgc ccgttcccct attgcttaag gatcaaagtg aaattgtagt tattattgaa
    17581 gataatggct ggccaggacg aggaaatttt gtagcaacta cagttcgtac taccctggaa
    17641 gataactgga caaaaggcta tgtagatgga aatagctcaa aacgttcaat gatatttcag
    17701 attcctccac cgacaaatat tgtgtctgct gctccatata ttcgaaaatt tccctgggga
    17761 gaaaccattg cttcttatca aagcaatgaa aatcgcattt ccaacgattt acaatatttc
    17821 gatatgtatg ttgtaatagg tgacgagcga gcacgtaatt tcaaagcaaa aagtgctcct
    17881 ttttcgttag gaaatgataa acatgctatt tggaattcaa tatcagtgat tgatacagga
    17941 attatagtag ctttaggttc aattggtccc ccttataaat caaatgatgt tgtaatgatg
    18001 aaaggttatc ctcttcgtca agcaaaagct aattatggcg taattaatgt agatggaaca
    18061 aaaagtttag atgagaagtg gacaacttcc aatgcatctc aattatattt tggtcatata
    18121 actaaaaata aagctactgt agatttttta tatgatgaaa aatatcttta tttaacttct
    18181 caaataattg acagaaatat aattaataca gggttagata atgacggtat tcgctttatg
    18241 atcgatgctg ataatatttc tggttctatg ccagaaaccg gaatgtatag ttttttcttt
    18301 gatactaatg gaaccgttaa atttcaacgt gggaatcacg gcatttggac aacagataat
    18361 aatgtttcag aaattttata tgctgttgat ataaaatcat tttattacaa tattgaagct
    18421 gctattcctt ggagccttat tggaaaatca gctcctcctt ttggcgaaag aatggcaata
    18481 gcgatagaga tagtcaataa agaggaatat actttaacaa aagaaggcat agctgatgtt
    18541 gacaataatg cttcttggac atggttagag tttcagctta tccctcgcca aagtaataaa
    18601 aatatctgtg taaatgaaga taattccgtt ataaaaactt ttgtaaataa taatattttg
    18661 tatataaata gtcctgtaat gataaaagaa ttatctcttt tttcacttga aggaaaatta
    18721 attgataaaa aaaaagaagt tggactaaat tttcatatcc ctatacctat tcctttttat
    18781 aaaggaatag tccttatgtt ttctcttgaa aatggagaaa taataaataa gaaaatttta
    18841 ttttaattga aattttattg agtttgtgat catttatttt tttaatttat ttttctgcta
    18901 gctgtaagaa atgaagatta ctttgactta aaaatgaaca taaagatagt gaaaacaaga
    18961 atataacagg agggtttcct cctttagaaa cttgttatgt agttttttct taaaattaat
    19021 aactaaaaat caagaaatat taaaattgat tttccttaat agactcataa acgacaaatt
    19081 taacacaaaa ttagcctaaa aaataaaatt atgtacaaat ttgagttttt ctttaattta
    19141 ttgcttttta taattttttt taaaagtaat tatataaagg gggaaaattt tgttaccacc
    19201 gtttcaatag aaaaaaaagt tcccctcgca gatccatata ttttactgta cgatggaatg
    19261 tattatgctt atggtacagg tgcagaggat ggaattgaag tgttgacgtc ttctgatctt
    19321 aaaagttgga aacggactgc aggtacacca actgggttag ctcttcataa agatgattct
    19381 tacggggaaa aatggttttg ggcaccagaa gtatattttg tgaatggaaa attttacatg
    19441 tattattcag ccgaagaaca tatttgtgta gctatatctg attctccttt gggaccattt
    19501 cgacaaacag agcaaaaacc tatgctggaa gaagagaaag ccattgacaa ttccttgttt
    19561 actgatgata acgggaaagc gtatttgttt tttgtccgat tcaatgatgg gaacaatgtt
    19621 tgggtagctg aattggaaaa cgatttgatg accttgaaaa aggaaacgat gcatcattgc
    19681 atcagtgttt cacaatcctg ggaagaagtt tggcccagag tgaacgaagg accatttgtt
    19741 attaaacata atggcattta ttatatgact tattctgcca acagttacga aagtcctttt
    19801 tatggaattg gttgtgcaac tgcgacaaat attatgagag aatggacaaa atatcctgat
    19861 aatccattat tacaaaaccc aggagatttg gttggcgttg gacataattc ccttttctgg
    19921 gacaaggatg gcaacttgcg gattgtattt catgctcatc atgataaaaa cactattcat
    19981 ccaagaaata tgtatattag taatgctggt tttaagaacg taaatggaat agataaattt
    20041 tatatagatc caaattatca aacgccggta ttagaggtaa acaaataagt gataatttct
    20101 ttttcgaagc gataaggtat ttgtttaaaa ttatttataa tgagataaat actttatcgt
    20161 ttttttatta tattcgtcct aaatttttat ttcctttttt attgatttat atgttaaaat
    20221 ataaaaactt gttttttgtt gttttttttc ttatattatc tttcacactt cctgtgtggt
    20281 ctttaccttc ctatcagttt aaacattatg atataaataa gggattatca caaaatacag
    20341 tgctgtcaat tctccaggac aaacagggtt ttatgtggtt tggtacgaaa catggtttaa
    20401 atagatttga tggaaaatca ttcaaagttt ttaaattttt tcctaatagt gaagtaaagg
    20461 ataatgtttt tcggtgtatt ttgcaggata aggacgaaaa attatggttg ggaaccgatg
    20521 aaggcgttt
//
LOCUS       DAOSAS010000013        19822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_1930_length_19822_cov_11.694845, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000013 DAOSAS010000000
VERSION     DAOSAS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19822
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1930_length_19822_cov_11.694845"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(467..1708)
                     /locus_tag="PLR72_01240"
     CDS             complement(467..1708)
                     /locus_tag="PLR72_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOK35868.1"
                     /translation="MKFEYFIAKRIHFQQGKKNVSRPAVRIATIGIALGLAVMLISVC
                     VVIGFKSEVRNKMIGFGGHIQITNFDNNNTYEMIPIQMDSLLIQKISEIKGVKGIQKF
                     ATKPGIIKTADEFQGIILKGVDKNYDWNFFKSNLVEGSIPDLFSETPKNEILISRYLA
                     NLLKLKLDDQFYTYFLQDQIRARKFKIVGIYSTNFVDYDKMFLITDIRHVQRLNNWSS
                     DMFGGLEVLIDDFDHLDEIGEKVTFLTANRFTKEGNAYFTQTIKQLNPQIFSWLDLLD
                     MNVWVILALMMAVAGFNMISGLLILILERTSMIGILKAMGATNWQIRKIFIYHSFFLV
                     GKGMLWGNLIGLAICALQYFTHIIPLNPEAYYVASVPIVFNWMYILLLNIGTFLVSIL
                     IMIAPSYLITKISPAKIIRYE"
     gene            complement(1812..3014)
                     /locus_tag="PLR72_01245"
     CDS             complement(1812..3014)
                     /locus_tag="PLR72_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOK35869.1"
                     /translation="MPHTSQRGLSMPESPIRKLAPLADKAKARGIKVYHLNIGQPDLK
                     TPQKALDVLKTIDRTILEYSPSDGFKSLKIKLASYYEKFNIDVTEEDIIITTGGSEAV
                     NFAFMSCLDPGDEIIVPEPAYANYTAFAIAAGVVVRPVISTIDDGFALPSIEKFEELI
                     TPKTKGIMICNPNNPTGYLYTRSEMNQIRDLVKKYDLFLFSDEVYREFCYSGAPYISA
                     FHLDGIEENVILADSFSKRYSECGIRVGALVTKNKEVRKNVMKFCQARLSPPLIGQII
                     AEASIDTPQEYMLESYNEYLERRKFLIDGLNRIPGVYSPIPMGAFYTVARLPVDDADK
                     FCEWMLSDFEYEGATVMLAPGSGFYTLEGMGKDEVRIAYVVNKEDLKKSLIILAKALE
                     VYPGRTNK"
     gene            complement(3168..5645)
                     /locus_tag="PLR72_01250"
     CDS             complement(3168..5645)
                     /locus_tag="PLR72_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK35870.1"
                     /translation="MNKRLSFLFCFFVVTLILTAQNKVRIFGYVVDSNNRGIELVNVF
                     LKGTTTGTTTNQNGYYELITEIKDSAEIIFSMVGYQTVSYTIHPKAKVIQLTVELPAL
                     NQEIKEIDIIGQKRQTNTIDVLNPSKYRLMPNPSGGIESLLIAFTGVTSSNELSSQYN
                     VRGGNFDENIVYVNGIEIYRPLLIRAGQQEGLSFINSDMVQNVSFSSGGFGVEYGDKM
                     SSVLDIQYKKPTRTEASVSLNLLGASAYVGTASKRFTQMHGIRYKTSAYLLGTLETKG
                     EYNPSFVDYQTYMTYQLKPKWELTFLGNFSQNSYQFIPQTRETSFGTYNMGRKLTVFF
                     GGQEKDLFQTAFGAFTLNYHPLKETKLSLIASGFRTNEKETYDISGEYILSEIKMEDD
                     LSKETGETLGIGNYHEHARNRLNATVMNISHQGESKAGNHQLKWGALLQFEKINDKIN
                     EWEWRDSSGYSLPYHPYDVKLYYNLKALNSLSSTRSSVFLQDTYRYSSIAGTLSFTGG
                     LRASYWSFNDEFLLSPRLSFSYLPHWEKDFSFRFATGIYYQSPFYKEIRDTITDNLGN
                     VNVALNSHIKSPRSVHFVLGGDYYFRALGRPFKFTTETYLKLTDRLISYSIDNVKIVY
                     SGKNDGKAYTAGIDFKLFGELVPGTDSWINFSLMRSREDLINDSYVKNTYDENGNLSS
                     SEIVNPGWIPGPNEQRYSFSMMFQDYLPTDPRYKLQLKFIWSDGLPFGPPQNYQFRSV
                     FRMPPYRRVDIGASRLLVNGKDKWMEKTWLKPVKDIWLNVEVFNLLDFKNVNSYYWVT
                     DVYGQQLAVPNYLTGRQFNIKLIVDFK"
     gene            complement(5744..6733)
                     /locus_tag="PLR72_01255"
     CDS             complement(5744..6733)
                     /locus_tag="PLR72_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK35871.1"
                     /translation="MCILSTVDLTIGYLKKDKKKVVQSNLNLELYAGKLTGLIGPNGV
                     GKSTLLRTLSGLQKPLGGKVLLENKELSKLSYQEKATKIALVLTDKIDILNATVYDIV
                     SYGRYPYSNWLGNLTDKDHIYIEQAIQMVHLEEKRNELLNELSDGERQRVMIARALAQ
                     DTPVILLDEPTSFLDVSNRIEIMLLLHKLAHQTQKAILLSTHELDLALQAADSIWLMD
                     KNGKTICGVPEDLVFNGSFARAFASKYYFFNEANGNFSMNFSLNKQVAVTGNKTRMYW
                     TLRALARAGFEAKEEAPVKILVTEDGWILNDEKINSMEELLDKLTELEKLNLS"
     gene            complement(6781..7779)
                     /locus_tag="PLR72_01260"
     CDS             complement(6781..7779)
                     /locus_tag="PLR72_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOK35872.1"
                     /translation="MSKRQKNSLFFLLIFITLAFFVLDLAWGSIRIPFNEIFSILAGK
                     GTNDIHTEIILNFRLPKAITAIIAGISLSIAGLMMQTLFRNPLADPYILGISSGASLG
                     AAMVMMAASLFPAVFISSNWSLIIAAVIGASLVLLLVILASFKVHDVVSLLIVGIMFG
                     TIASALVNIFQNLSNPDAVKLFIMWTFGSLSAVTWHHLLVLIPVFLIGMMIAIFLQKR
                     LDVLLLGENYARSSGVSIQQTRFLIVLATGLLAGGITAFTGPIAFVGIAIPHVARGIF
                     RTSSHKILIPACVLCGASLLMICDIISQIPAYSLPINTVSALFGAPVILWIILKRK"
     gene            complement(7976..9202)
                     /locus_tag="PLR72_01265"
     CDS             complement(7976..9202)
                     /locus_tag="PLR72_01265"
                     /inference="COORDINATES: protein motif:HMM:NF023776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HOK35873.1"
                     /translation="MRGEPVIVKQLLRKAKDSALLAIEFYNKPAVSFKSEGFITMMCI
                     AWTSLFHAYFFKNKIKPYYKKNENSKRSRYATTPIKLLNGKTINEFKWWELTECLNKY
                     YQSNTNHPVKKNLEFFCEIRNMIVHRNIPELDSNLYAECQANILNFNNFLKEHFGDKH
                     SMDYLLSFTIQMFKSNKNFFDATNSELKKKNAVEIVEFIKSFRSSLSSDVFESPEYAY
                     KAVLIQVKNHESKDALPLKFINEKDLTDEQKEDLKNIMGVVLVKEKTGYKNKIPENFS
                     LTSTDLYNSIKERYPYIRLNNNYNKIKCYLRIKYDNVLAYEWLNNPKSKKSKSFTYYY
                     DPKIIDEFGKIYPPITESNKTIFEQVIQVIDQYLSVKKENPSADTSALEHQIDRLVYQ
                     LYDLTEEEIKIIGGEN"
     gene            complement(9309..13121)
                     /locus_tag="PLR72_01270"
     CDS             complement(9309..13121)
                     /locus_tag="PLR72_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOK35874.1"
                     /translation="MDDKKEIINILDKIINDFSIDNLVRLFQNKTTKFRMLQSPASQP
                     LFTDGLLLGEFDCGDESVCIFTFKTHQPLTERSGKKAQYELAKKMLKEEIRYDGGFFI
                     FYDEQGNFRFSLVYDIPLSSGTREWSNFKRYTYYVSKNQTNKTFIQQMAEADFSSFNN
                     IIEAFSVEKVTREFYNEIANWYFWALQKVKFPEDAEQQPNGRNIALIRFITRIIFVWF
                     MKEKKLISPQLFDFDHIKNILKSTDDNESTYYKAILQNLFFATLNTPIPERKFRTENR
                     FQGKNEDYMNHEYFRYQELFSDPKQDINLLFNEIPFLNGGLFECLDKPKNDPSNDTGD
                     EIRMDGFSDKKENQPIFPNELFFSKEKNVDLTEYYGDTKYKNTRVQGLISILNAYNFT
                     VDENTPLDEEVALDPELLGKVFENLLASYNPETATTARKATGSYYTPREIVDYMVTES
                     LKHYFKTSFENTFRTENLDEKLISLFSYSKEENPFNEKETDFIIEKINGLKIIDPAVG
                     SGAFPIGVLNKLVFLLSKLDPHNEKWKTQQFRALDYITDPTLKNELREKIENNFKRNE
                     LDYGRKLYLIQNCIYGVDIQSIAIHIAKLRFFISLLVDEKVNSNIEDNFGIEPLPNLE
                     TKLIAANSLIGLHKPQQLIIKDDKIVQTEKELSQLRKQHFTANNEKKKKEIEKRDREL
                     RNRLKELLEGSGFPSDYTEKIASWDPYDTNQSAKWFDPEWMFGVKKEKKKIQKDWKTF
                     NITWATHNSRRKENYMPSNTEGLAPVLLSLEERNKVAQLLAWRIKAKHYRVLAMNVLT
                     DHVHLLLVAEEKDVPKIVGDLKGYVSHEFGTEATHGLSTPTHGLTRGSGSDCTYSDGT
                     QQKLWAKHYNNTPVEDFEHFANVIEYINYNHLKHLIPAIDFSLFQDALTPIDKAFEPV
                     EEETGFDIVIGNPPYGIKFSEDEKKYFKEQYLCTKTIPGKQKGSLDSFALFIEKGFNI
                     LKKNGNLIYIVPISITSSDSMTALHNLLEQNCNLIKISSYAVRPQPIFQNAVINTSIL
                     FFIKTLTPVEQILCTKMYRKSKGFDLKTLFENLEFIDIKKYKLFGRYPKISTPIETQI
                     LDKLFSIKTKTKDLIRENGKPIYYRTTGGRYFKVITNYSTGSTKEKDMLIDKHFCNAT
                     GTILSSNLFFWYYQIYSNNLDLKSYEIESFPIPINELTEERIKVLEVLYDEYLKDIEE
                     NAKIRITTKYAHIDSFKEYKIGKSKKYIDAIDDYIGPLYGLNKEEIEFIKNYEIEFRL
                     TENEEDEN"
     gene            complement(13114..13410)
                     /locus_tag="PLR72_01275"
     CDS             complement(13114..13410)
                     /locus_tag="PLR72_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35875.1"
                     /translation="MGYYVEAIHELPLQQQNTREIRRKMLLPKIIGYFKMNAAKQINK
                     LQQSQNQLIWQRNYYEHIIRNDNELNRIRQYIFDNPLKWENDKNFITINQKTYG"
     gene            complement(13395..13631)
                     /locus_tag="PLR72_01280"
     CDS             complement(13395..13631)
                     /locus_tag="PLR72_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35876.1"
                     /translation="MTTFQNKYRIESIRLPGYDYSRPGAYFVTIVTYQRQCLLGNIFN
                     GEMILNECGEIVKNWWQEIPNHYSNVQLDKWVIM"
     gene            complement(13612..17022)
                     /locus_tag="PLR72_01285"
     CDS             complement(13612..17022)
                     /locus_tag="PLR72_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOK35877.1"
                     /translation="MNNFITNSTKKQLRTRLIELLSKSEELKFLVGFFYFFGLRELYD
                     GLQSNDKVKLKVLIGLNVDKTIHGLTEYGEKDKKLSDDERIENFFTSIRKSINSDVFD
                     HKDFYEQVKFFLQKIKNDEIILRKTREPNHAKLYIFKLEEDQIGRNRLFITGSSNLTS
                     AGLMSQAEFNVEISDYGFDEAESYFDELWNNAIKITENDELKEKIVKVIEQETLVKEI
                     TPFEAYLFVLKNYLDTFRGKEVTRRLTEVLEENGYKPYRYQLDAVQQALSIIENNNGV
                     IIADVVGLGKTVIACSIAFELKKRGIVIAPPGIIGDKAKSEGWKKYLEQFHMASLGWE
                     AFSLGDLEHVSEFVSKTNDIEVVIIDEAHRFRNEDTKDYEYLKNICRDKIVILLTATP
                     FNNKPADIFSLLKLFIVPKKSSITLDDDLENKFRTFKSEFDKLSYIKRYFQSSDRQKR
                     NRALSYYTDLFGEKIVDSTKIKKRTEILAKQIRNVIEPVIIRRNRLDIQNHPYYKNEV
                     NELSIVESPKEWFYELNKEQSVFYDKIIQQYFALPDDGGRFKGAIYKPFEYELQKVKK
                     GELGKEENFQYLQQFNLYDFMRRLLVKRFESSFGAFEQSVKNFINIHQIVLEFIQKTD
                     KYILDRNLLEKIYELDADEIENYLQEYSEKIKNNEYPKNHKVYELKKFERETDFINDI
                     QSDIALFQQILGELHKLNLVAKDPKAERLIEEVKTVLDAPPENLFEPKRKVIIFSEYA
                     DTVNYLAPLLTQAFNGRVLVVAGNLPASKIKEINKNFDATATQQADDYNILLTTDKIS
                     EGFNLNRAGMVINYDIPWNPVRVIQRIGRINRISKKVFDKLYIINFFPTEKGSDLVHS
                     REIAAQKMFMIHNTLGEDAKIFDIDEEPTPSGLYNKIQQSPDSLEGESSYTKISKEFE
                     AWKEKYPDMVASLKDFPSRIKVAKENTENELFVVIKKQKLFVHHQLYDAKEGSENHFI
                     KEVEDVLEKIKADKNELPLPLSNQFWEEYETIKNYQENPKNNKPNPENSLQNKAIIVL
                     RNLLAINDNFQVTLLKPFVRMLLEDLLDYGTLSDYTIRRIANIDIEKNFTKAVDELKS
                     IEKGLGSDYLYKEKQRISDFSKEIIIAIENRKTNELPTQNTFNDNISK"
     gene            17508..19820
                     /locus_tag="PLR72_01290"
     CDS             17508..19820
                     /locus_tag="PLR72_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HOK35878.1"
                     /translation="MNKYLQHDPWCIIEEGFHMDSQLASESIFSLGNGHMGQRGNFEE
                     YYSGETMFGSYIAGIYFPERAERSNWKNGYSETNDKIVNAPNWICISVRLNDERLDLG
                     SWDIHNFRRVLNMREGYLERTFEVTSPKGHRIQATVRRFLSMAENEVAVISYTVKSIN
                     YEGRISFLPLIDGNVRNYYTNYNEPFWNVLQTKTEQDVSHLWAQTRRLDFHVCAALTY
                     EFYKNNMLLNVNPIKIEKERVAGFSVGTDIKKGETVCLKKYVSVMSSLNHPREELTDR
                     ACNLARAVKQKGWDKLFDEHKAVWKEKWLQSDVIIENDIAAQQAIRFNIFQLNQSYSG
                     NDDRLNISPKGFTGEKFGGGTRWDTEAVCVPFYLSTSPQNVARNLLLYRYRHLPKAIQ
                     NAEKLGFTNGAALFPAATFNGSECLNEWEISFEEVFRNGIIAYAIYNYLRYTDDNDFL
                     VNYGLRILIAIARFWAQRVNISHQKKKYVLLGVTGPNTYENNVNNNWFTNFIAVWCLK
                     FTMEAIEYVKKKDFRAFGDLSQSINFSNEEVFLWKDITENMYFPEDKKLNIFLQQDGF
                     FDKTLVKASEIPASQLPLNQHWTWDRILRSPFIKQADVVLGLYLFEDLFDKETIRQNF
                     DFYEPFTVHESSLSPCIHSIVASSIGEIEKGYHLYLRTARLDLDDYNDEVADGLHITS
                     MAGTWLSIVQGFGGMRVIDNHLHFNPHIPEKWEAYAFNVIFRGTQLKIRVEKKKVILQ
                     RIKGEPIDLYVYNNLIHLSGNECEEIERKN"
BASE COUNT         6228 a         3398 c         3216 g         6980 t
ORIGIN      
        1 ttacgcagtg cttctatctg ttctttaatg ctcatttcca taatcaaaat gtgtttctaa
       61 aaataaatcc aagaatttat tgaactgttt tataccaagt tgtaatcatt aatataattt
      121 taatagcatc gattgatttc gattaaatga ttttttacga tagatatttg tttagcaaaa
      181 tgtttgattt tatcagtagt taaaaatcag gagattttat tttttgtgcg acgtagccaa
      241 aaggctacgc caaacaaaga tatgaggctt tgccacaaac cccacttact tttttattat
      301 tttcagtaga gacgttgcat gaaacgtctc tacaaaaacc acaaactatt gattatcaac
      361 taaatattaa aaattctaaa ctcctgactc caaactcgta tctcatatct cgtatctcaa
      421 aactcatttt catttttaaa agccattttg ctattaaatt aaactttcat tcatatcgaa
      481 taattttagc gggcgaaatt tttgtaatta aatatgatgg agcaatcata attaaaattg
      541 aaaccaaaaa agtcccgata tttaataaaa gaatatacat ccaattaaaa acaataggca
      601 cacttgccac ataataagct tctggattta aaggaataat atgggtaaaa tattgtaacg
      661 cacaaattgc cagaccaatc aaattccccc acaacattcc tttgcctacc agaaaaaaag
      721 aatgataaat aaatattttt cttatttgcc aattggttgc tcccatcgct tttagtatgc
      781 caatcatact ggtgcgttca aggatcaaaa taagcaaacc tgaaatcatg ttgaaacctg
      841 ctacagccat cattaaagcc agaataaccc aaacattcat atccagcaaa tcgagccagc
      901 taaaaatttg aggattcaac tgtttaatag tttgagtaaa ataggcgttc ccttctttcg
      961 taaacctatt tgcagtaaga aaagttactt tttcacctat ttcatccaga tgatcaaaat
     1021 catcaatcaa tacttccaac ccgccaaaca tatctgatga ccagttattt aaacgttgaa
     1081 catgacgaat gtcagtgatt aaaaacattt tatcataatc cacaaaattt gtagaataaa
     1141 tgcctacaat tttaaatttt cttgctcgaa tctgatcttg caggaaataa gtataaaatt
     1201 gatcatctaa tttcaatttt aataaattgg caagatatct ggagataaga atttcatttt
     1261 taggtgtttc cgaaaataaa tccggaatgc tcccttcgac cagatttgat ttaaaaaaat
     1321 tccaatcata atttttatct acacctttta aaatgatacc ttgaaattcg tcagccgttt
     1381 taattattcc cggtttggta gcgaactttt gaataccttt tactcctttt atctccgaaa
     1441 ttttttgtat cagcaacgaa tccatttgaa tgggaatcat ttcgtaagta ttattattgt
     1501 caaaattagt gatttgaata tgaccgccaa aaccaatcat tttgttgcgg acttcacttt
     1561 tgaaaccaat aaccacacaa acggaaatca acatcacagc cagtcccaat gcaattccaa
     1621 tggtagcaat ccgaactgct ggacgagaaa catttttctt tccttgttga aaatgaatgc
     1681 gttttgctat aaaatattcg aatttcaaat cagcgaataa ttttcaaaat attacctttt
     1741 tgaaacaggc cagaaattgg gaaaattata aagcaagaat ggaaaaattc caaataattg
     1801 attttcaacc atcatttatt tgttcttcct ggatatactt ccaatgcttt agctaaaata
     1861 atcaaagatt ttttcaaatc ctccttattt actacataag caatccgcac ttcatccttg
     1921 cccattcctt ccagcgtgta aaaaccagaa cctggtgcca gcataacggt agctccttcg
     1981 tactcaaaat cagaaagcat ccattcacag aatttatcag catcatcaac aggcaagcga
     2041 gctacggtat aaaacgctcc cattgggata ggcgaataaa cgcccggaat gcgattcagt
     2101 ccatcaatta aaaatttacg tcgttccaga tattcattgt aagattcaag catatattct
     2161 tgtggtgtat caattgaggc ttctgcaata atttgcccaa ttaatggcgg acttaatcga
     2221 gcttgacaaa atttcattac attttttcta acttctttgt ttttggtcac caacgctcct
     2281 acacgaatac cacattcgct gtaacgtttt gaaaatgaat cggccaaaat aacattttcc
     2341 tcaataccat ccagatgaaa ggctgaaata tatggtgctc cgctatagca aaattcgcga
     2401 taaacttcgt cagaaaacaa aaataaatca tatttcttta ccagatcacg aatttgattc
     2461 atttctgaac gcgtgtataa gtaacctgtc ggattattcg gattacatat cataatgcct
     2521 ttcgttttcg gagtaatcaa ttcttcaaat ttttcaatgg aaggcaaagc aaaaccgtca
     2581 tcaatggtgg aaatgacggg gcgtacaacg acacctgcag ctatggcaaa agctgtataa
     2641 ttggcataag caggttctgg cactataatt tcatcgcccg gatccaaaca actcataaaa
     2701 gcaaagttga ccgcttctga acctcctgtt gtgataataa tatcttcttc tgtaacgtca
     2761 atattaaatt tttcataata actcgccaac ttgattttta aacttttaaa accatcactt
     2821 ggactgtatt ccaaaatagt gcgatcaatt gtttttaaaa catctaaagc tttttgtgga
     2881 gttttcaagt cgggctgacc tatattcaaa tgataaactt tgattcctcg tgctttggct
     2941 ttatcagcca gtggagcaag tttacgaata ggagattcag gcatggaaag cccacgttgt
     3001 gaagtgtgtg gcatttttaa aggtgttatt ttgatatttt atgatagttt ttattctaaa
     3061 tatttttaaa aaaaatttag ttacaaattt agcaaaattt acagaaaatt ctatttgctc
     3121 tgaaattaaa atcgaaacgg atactggaag tatttttgtt tttattttta tttaaaatcc
     3181 acaatcaatt ttatattaaa ttgtcttcca gtcagataat tgggaacagc caattgctgc
     3241 ccatacacat ccgttaccca ataataggaa tttacatttt taaaatccag taaattaaat
     3301 acttccacat tcaaccagat atctttgaca ggtttcagcc aggttttttc catccattta
     3361 tcttttccat ttaccaacaa gcgagatgct ccaatatcca ctcgacgata aggtggcatt
     3421 ctgaaaactg agcgaaactg ataattttgt ggcggaccaa atggcaaacc atctgaccag
     3481 atgaatttta attgcaattt atagcgagga tcggtaggca aataatcctg aaacatcatg
     3541 gagaaggaat aacgttgttc gttgggtcct gggatccagc ccggattaac tatttcagaa
     3601 gaagataaat ttccattttc gtcataagta ttttttacat aactatcgtt aattaaatct
     3661 tctctcgacc gcattaacga aaaattaatc caggaatctg ttcctggaac caattccccg
     3721 aaaagtttaa aatcaattcc cgcagtataa gctttaccgt catttttgcc agaataaaca
     3781 atttttacat tgtcaataga ataagaaatg agtcggtcgg tgagtttgag atacgtttcc
     3841 gtagtaaatt taaaaggtcg tcccaatgcc ctgaaataat aatcacctcc caatacaaaa
     3901 tgcacagaac ggggcgattt gatatgagaa tttaatgcta catttacatt tccaagatta
     3961 tcggtaattg tatctcttat ttctttataa aaaggtgatt gataataaat tcctgtagca
     4021 aaacgaaaac taaaatcttt ttcccaatga ggcaagtaag aaaaagatag acgcggactc
     4081 agcaaaaatt cgtcgttaaa cgaccaataa cttgctcgta aaccacctgt gaatgacaaa
     4141 gtgcctgcta tagaactata tctgtaagta tcctgtaaaa aaactgacga acgagtggat
     4201 gacaaggaat ttaaagcttt cagattataa tataatttta catcgtaagg atgataaggt
     4261 aacgaataac ccgacgaatc gcgccattcc cattcgttga ttttatcatt gattttttca
     4321 aactgaagaa gtgctcccca ttttaattga tgattgcctg ctttcgattc tccttgatga
     4381 gaaatattca ttaccgttgc attcaaacga ttacgagcat gttcatggta attaccaatt
     4441 cccaatgttt cgcctgtttc ttttgataaa tcatcttcca tttttatttc actcaaaata
     4501 tattctccag aaatatcata agtttctttt tcgttggtac gaaagcccga agcaattaaa
     4561 ctcaatttag tttcttttaa aggatgataa ttcaacgtaa aagctccaaa agctgtttga
     4621 aataaatcct tttcctgtcc gccaaaaaat acggtgagtt ttcttcccat attgtaagta
     4681 ccaaaagatg tttctctggt ttgtggaatg aactgatagg agttttgtga aaaatttcca
     4741 agaaaagtta attcccattt gggcttcaac tgataggtca tataagtttg ataatctaca
     4801 aatgaaggat tatattcgcc ttttgtttcc agcgttccaa gcaaataggc agaagtttta
     4861 tatcggatgc catgcatttg agtaaaacgt ttgctggctg tgcccacata agcagaagca
     4921 cctaataaat taagcgaaac agaagcttcc gttcttgtcg gttttttgta ttgaatatcc
     4981 agcacggatg acattttatc accatattct acgccgaatc cacctgaaga aaaagacaca
     5041 ttctgcacca tatctgaatt gatgaaactc aatccttctt gttgacctgc ccttattaac
     5101 aacggacgat aaatttctat gccattgaca tatacaatat tttcatcgaa atttccacct
     5161 ctaacgttat attgggaact aagttcattt gatgaagtaa ctcctgtaaa agcaatcaat
     5221 aaagattcaa taccccccga aggattaggc atcaatcgat atttggaagg atttaatacg
     5281 tcaatagtat ttgtttgacg tttttgccca ataatatcta tttcttttat ttcttgattc
     5341 agagctggta attcaacagt taattgaata acttttgctt ttggatgaat ggtataagaa
     5401 acagtctgat aaccgaccat cgaaaaaata atctcagccg aatctttaat ttcggtgatc
     5461 aattcataat agccattttg gttggtggta gttcctgttg ttgtaccttt caaaaaaaca
     5521 tttaccagtt caatacctct gttgttagaa tcaacaacat agccaaaaat ccgaacctta
     5581 ttttgtgcgg ttaaaatcaa cgtaacaaca aaaaaacaaa aaagaaagct caatcgcttg
     5641 ttcatgcaat gttattaata agttacccta aaaacgaaaa aagggaatga ttattttact
     5701 tttcctttct atcttctttt gaatgatttg taaaaaatgt tttttatgat aaatttaatt
     5761 tctccaattc cgtcaattta tcaagcaatt cttccataga gtttattttt tcatcattca
     5821 aaatccaacc atcttcggta accagaattt ttactggtgc ttcttctttt gcttcaaaac
     5881 ctgctcgtgc caatgctctg agtgtccaat acattctggt tttgtttcct gtcactgcca
     5941 cttgtttgtt caaagaaaag ttcatggaaa aattaccatt ggcttcatta aaaaaataat
     6001 acttactggc aaaagctcga gcaaagctcc cattaaaaac caaatcttcc ggcacaccac
     6061 aaatcgtttt tccattttta tccatcaacc agatactatc cgcagcctgt aaggccaaat
     6121 caagttcgtg agtggaaagc aaaattgctt tctgcgtttg atgagccaat ttgtgtaaca
     6181 acaacataat ttctattcga ttggagacat ccagaaatga ggttggttca tccaataaaa
     6241 ttacaggagt atcctgagcc aatgctctgg caatcattac cctttgacgt tcaccatccg
     6301 aaagttcatt taacagttca ttcctttttt cttctaaatg caccatctga attgcctgtt
     6361 caatatatat gtgatcttta tcagttaaat ttcccagcca attgctgtaa ggatatcgac
     6421 cataagaaac aatatcataa accgtagcat ttaatatgtc aattttatca gtcaaaacaa
     6481 gagctatttt tgttgctttt tcttgataag aaagtttaga taattcctta ttttcaagca
     6541 aaacttttcc accaagtggt ttttgcaagc ccgataaagt tcgcaataaa gtagattttc
     6601 ctacaccgtt aggaccaatt aatcctgtta atttcccggc atacaactcc aaattcaagt
     6661 tggattgtac tacttttttt ttatcttttt ttaaataacc aatcgttaaa tcaacagtgg
     6721 acaaaatgca cataactcac aatcaataat tttccagcaa taaatgaaaa aacaattttc
     6781 ttattttctt tttaaaataa tccacaaaat aacaggtgca ccaaacaatg cacttactgt
     6841 attgataggc aacgaataag ctggtatttg agaaataata tcacaaatca tcagcagcga
     6901 agcaccacaa agcacacaag caggaattaa aattttatga gaagaagttc gaaaaattcc
     6961 tctggcaaca tgaggaatag ctatccccac aaaagcaatg ggtccggtga aagcagtaat
     7021 tcctcctgcc aataaacccg ttgccagaac aattaaaaaa cgcgtttgct gaatagaaac
     7081 tcccgagctt cgtgcatagt tttctcctaa tagcaaaaca tccagccgtt tttgcagaaa
     7141 aatagcaatc atcattccaa tcagaaaaac aggaattaat accagcaaat gatgccaggt
     7201 tactgcactc aaactgccaa aagtccacat aatgaaaagt tttaccgcat ccggattgct
     7261 caaattttga aaaatattga ccagcgccga cgcaatagtg ccaaacataa ttccaacaat
     7321 caacaaagaa acaacatcgt gaactttaaa ggaagccaaa ataaccaaca gcaaaaccag
     7381 cgaagcacct ataacggcag ctataattaa cgaccaattg ctactgataa aaacagcagg
     7441 aaacaatgaa gctgccatca ttaccattgc tgctcccaaa cttgctcccg aacttattcc
     7501 caaaatgtaa ggatcagcca gcggatttct aaaaagtgtt tgcatcatta atccggctat
     7561 ggaaagcgaa attccggcaa taatagctgt aatagctttt ggcagacgaa aattcaaaat
     7621 tatttccgta tgaatatcgt ttgttccttt gccagctaaa atggaaaaaa tttcattaaa
     7681 aggtatgcga atgcttcccc atgccagatc taaaacaaaa aaagctaatg taatgaatat
     7741 cagaagaaag aacaagctat ttttctgtcg tttagacatt taatattaat tttgataatt
     7801 attttccaca aaataattca aataaatagt aacagactaa ctataaaaat ttacttttat
     7861 atcaacaggt tttattcaga actaattaaa tataaaacat attttttaac taaaataatt
     7921 tatttatccg tatagttcct ccactttttg tgtaaggata tttgaatttt attattcaat
     7981 tttctcctcc tattattttt atttcttctt ctgtcaaatc gtaaagctga taaaccagcc
     8041 ggtcaatttg gtgttccaat gcggaagtgt cggcggaagg attttctttt ttgacggaca
     8101 aatattgatc aataacttga attacttgtt caaatattgt tttatttgat tcagtaatag
     8161 gtggatatat tttaccaaac tcatctataa ttttaggatc atagtaataa gtaaaagatt
     8221 tagatttctt actttttgga ttattgagcc attcataagc gagcacattg tcgtatttga
     8281 ttctaagata gcatttaatt ttattataat tattattaag acgaatataa ggataccttt
     8341 cttttattga attataaaga tctgtgctgg ttaacgaaaa attttcgggt atcttatttt
     8401 tataacctgt tttttctttt actaaaacaa ctcccattat atttttaagg tcttcctttt
     8461 gttcatctgt taaatctttc tcatttataa atttcaaagg gagcgcatct ttagattcgt
     8521 ggttctttac ttgaattaat acggctttat atgcatattc cggggattcg aaaacatcgg
     8581 aagaaagaga agatctgaat gatttgataa attcaacaat ttcgacagca tttttctttt
     8641 ttaattcact attagttgca tcaaaaaaat ttttattact tttaaacatt tgtattgtaa
     8701 aagatagcaa ataatccatg ctatgtttat ctccaaaatg ttcttttaga aaattattaa
     8761 aattcaaaat attagcttga cattctgcat aaagattcga atcaagttct ggaatatttc
     8821 tgtgtacaat catatttcga atctcacaaa aaaattccag attttttttt acaggatgat
     8881 ttgtattaga ttgatagtat ttatttaaac actcagtaag ttcccaccac ttaaattcat
     8941 ttattgtttt tccatttaat agtttaattg gtgttgtagc atatcgagat cttttactat
     9001 tttcattttt cttataatag ggtttaattt tatttttaaa aaaatatgca tgaaagaggc
     9061 ttgtccatgc gatgcacatc atagtaataa aaccttctga tttgaagctt acagccggct
     9121 tattataaaa ttctatggct aataaagctg aatcttttgc tttccttaaa agttgtttta
     9181 caattacggg ttctcctctc atattgacct ccttttttat cttaaatata tatatccgtc
     9241 atttctttat taaatggtat ttgaagttgg tttaatttag ctttgggttt ttattgaaat
     9301 tttgataatt aattttcgtc ttcttcattt tctgttaatc gaaattcaat ttcatagttt
     9361 tttataaatt caatttcttc tttatttaag ccataaagtg gaccaatata atcgtctata
     9421 gcatcaatat acttttttga tttcccaatt ttatattctt tgaaagaatc gatatgagcg
     9481 tatttagttg ttatccttat tttggcgttt tcttcaatat cttttaaata ttcgtcatac
     9541 aacacttcta aaactttaat tctttcttct gtcaattcat ttataggtat cgggaaagat
     9601 tctatttcat aagattttaa atcaagatta tttgaataaa tttgataata ccaaaaaaat
     9661 aaattactac tcaaaattgt tccagttgca ttgcaaaaat gtttatctat aagcatatcc
     9721 ttctcttttg tagaacctgt tgaataattt gttataactt taaaatatcg tcctcctgtc
     9781 gttcgataat aaatcggttt cccattttct ctaattaaat ctttagtttt tgtttttatt
     9841 gaaaataatt tatccaaaat ttgtgtttct ataggtgtac taatttttgg gtatcttcca
     9901 aataatttgt atttttttat gtcaataaac tctaaatttt caaataatgt ttttaaatca
     9961 aaacctttac tttttcgata catttttgta cataaaattt gttcaactgg cgtaagtgtt
    10021 ttgataaaaa aaaggataga agtatttatc acagcatttt gaaaaatcgg ttgaggtctt
    10081 actgcataag aagaaatttt gatcaggtta caattttgtt ccaaaaggtt atgaagtgct
    10141 gtcattgaat cacttgaagt gatagaaata ggaacaatat aaattaagtt tccatttttt
    10201 ttcaaaatat taaatccttt ttcgataaat aatgcaaatg aatctaaaga gcctttttgt
    10261 ttaccaggaa tcgttttggt acataaatat tgttctttaa aatacttctt ttcatcttct
    10321 gaaaatttta ttccatatgg cggattgcca atgacaatat caaagcctgt ctcttcttca
    10381 accggttcga atgctttgtc aatgggagtc aatgcatcct ggaaaagcga gaaatcaata
    10441 gctggaatta gatgtttcag atgattatag ttgatatatt ctatgacatt ggcaaaatgc
    10501 tcgaaatctt ccacaggagt gttgttgtag tgttttgccc agagcttctg ctgcgtacca
    10561 tcgctgtatg tgcagtccga tccagaccca cgggttaacc cgtgggttgg agtagatagc
    10621 ccgtgggtcg cctctgtgcc gaactcatgt gatacgtagc ctttcagatc tcctacaatt
    10681 ttcgggacgt ctttttcttc tgctaccaat aacaaatgta catgatcggt aagtacattc
    10741 attgccagta ctctgtagtg ttttgccttt atacgccaag caagcaattg tgccaccttg
    10801 tttctctctt ccagcgataa caaaacagga gccagacctt cagtattgga tggcatataa
    10861 ttctctttcc ttcggctatt gtgtgttgcc cacgtgatgt tgaatgtttt ccaatctttt
    10921 tgtattttct ttttttcctt ttttacacca aacatccatt caggatcgaa ccacttagct
    10981 gactgattgg tgtcgtatgg atcccacgaa gcaatttttt cggtataatc ggaagggaaa
    11041 ccacttcctt ccagcaattc tttcagtcga tttctcaatt cccgatctct tttttcaatt
    11101 tctttctttt tcttttcgtt gtttgctgta aaatgttgtt ttcttaattg ggaaagttct
    11161 ttttcggttt ggactatttt atcatcttta ataatcagtt gttggggttt gtgtagcccg
    11221 ataagcgagt ttgcagctat taattttgtt tcgagattgg gtaaaggttc gatgccgaaa
    11281 ttatcctcaa tgtttgagtt tactttttcg tccaccagca aagagataaa aaatcgaagt
    11341 ttggctatgt gaatggcaat agattgaata tccactccgt aaatacaatt ttgtatcaaa
    11401 tacagttttc gtccgtagtc gagttcattt cgtttgaaat tgttttcaat tttttcgcga
    11461 agttcatttt ttaatgtggg gtcggtgata tagtcgagcg cccgaaattg ttgggttttc
    11521 catttttcgt tatgtggatc aagttttgaa agcaaaaaaa ccagcttatt taaaacgcca
    11581 atgggaaaag cacctgaacc gacagcaggg tcaatgattt tgagcccatt aattttttca
    11641 atgatgaaat cggtttcttt ttcgttgaaa ggattctctt ctttgctata cgaaaaaagt
    11701 gaaattaatt tttcatcaag gttttccgtt cggaaagtgt tttcaaaact ggttttgaaa
    11761 taatgtttaa gcgattctgt taccatataa tccacaattt cacgtggtgt ataataactt
    11821 ccggtggctt tgcgtgccgt agttgctgtt tcgggattgt aggaagccag caggttttcg
    11881 aatacttttc ccaacaactc aggatcgagt gccacttctt cgtcaagagg agtattttca
    11941 tctaccgtga aattgtaggc atttaaaata gaaattaacc cttgtactct ggtattttta
    12001 tattttgtat caccataata ttccgtcaag tccacgtttt tttcttttga aaagaaaagt
    12061 tcgttgggaa agatgggttg attttctttt ttatccgaaa atccatccat gcgaatttcg
    12121 tcgcccgtat cattggaagg atcgtttttt ggcttatcca gacattcaaa cagtccgccg
    12181 ttgagaaaag gaatttcgtt gaacagaagg ttgatatcct gtttgggatc agagaaaagt
    12241 tcttgataac gaaaatattc atgattcatg taatcttcgt ttttaccctg aaatctattt
    12301 tctgttctaa attttctttc gggaatgggc gtattaagtg tcgcaaaaaa taaattttga
    12361 agaatagctt tgtaataagt actttcgttg tcgtctgtgc ttttcagaat atttttaatg
    12421 tggtcaaaat cgaaaagctg aggactgatc aatttttttt ctttcataaa ccatacaaaa
    12481 atgatgcggg taataaaacg aataagagca atgtttcttc cattgggttg ttgctccgca
    12541 tcctctggaa atttcacctt ttgcagtgcc cagaagtacc agttagcgat ttcgttgtag
    12601 aattcacggg ttactttttc tacgctgaaa gcttcaatga tattgttaaa agaagaaaaa
    12661 tcggcttcag ccatttgctg gatgaaggtt ttgtttgttt gatttttgct gacgtaatag
    12721 gtgtaccgtt tgaaattgct ccattctctt gttccagacg ataatggaat atcgtaaacc
    12781 aaagaaaaac gaaaattgcc ctgttcatcg taaaaaatga aaaatccgcc atcataacgt
    12841 atttcctctt tcagcatttt ctttgccaat tcgtattgtg cctttttgcc cgagcgttcg
    12901 gttaaaggtt gatgggtttt gaaggtaaaa atacaaacac tttcatcgcc gcaatcaaat
    12961 tctcccaaca ataaaccgtc agtaaataat ggttgggatg caggcgattg cagcatgcga
    13021 aatttggtgg ttttgttttg gaacaaacga acaaggttat cgattgaaaa atcgttgatg
    13081 attttgtcta aaatgtttat aatttctttt ttatcatcca tacgtttttt ggtttattgt
    13141 tatgaaattt ttatcgtttt cccatttcaa aggattgtca aaaatatatt ggcgaatgcg
    13201 atttaattca ttgtcgttgc gaataatgtg ttcgtaataa ttccgttgcc aaatcaattg
    13261 attttgtgat tgttgcaatt tattgatttg ttttgcggca ttcattttaa aatatccgat
    13321 aatttttggc aataacattt tcctgcggat ttcacgtgta ttttgttgtt gtagaggcaa
    13381 ttcatgaatt gcctctacat aataacccat ttatccaatt gaacgtttga ataatgattg
    13441 gggatttcct gccaccaatt ttttacaatt tccccacatt cattcaatat catttcgcca
    13501 ttgaaaatat tgcccaataa acattgtcgt tgataggtga caatggttac gaaataggcg
    13561 ccaggtcggg aataatcata acctggtaaa cggatggatt ctatgcgata tttattttga
    13621 aatgttgtca ttgaatgtat tttgtgttgg caattcattc gttttcctgt tttcgatggc
    13681 aataatgatt tctttcgaga aatcggaaat acgttgtttt tctttgtata aataatcgct
    13741 ccccaatcct ttttcaatag attttaattc atcaactgct tttgtgaaat ttttctctat
    13801 gtcaatattg gcaattcttc tgatggtata atctgaaagc gtaccgtaat cgagcaaatc
    13861 ttccaacagc attcttacaa aaggtttcag caacgttacc tgaaaattat cgtttattgc
    13921 taacagatta cgtagcacaa taatggcttt attttgcagc gagttttcag gattgggttt
    13981 attatttttt ggattttcct gataattttt aatcgtttcg tattcttccc agaattggtt
    14041 actcaaaggt aaaggtagtt cgtttttgtc ggcttttatt ttttcgagaa catcttccac
    14101 ttctttaata aaatgatttt cacttccttc ctttgcatca tagagctggt gatgaacaaa
    14161 aagcttttgt tttttgataa ccacaaataa ttcattttcc gtgttttctt tggctacctt
    14221 tatgcgcgaa ggaaaatctt tcaacgaagc caccatgtca ggatattttt ctttccatgc
    14281 ttcaaattct tttgatattt tcgtatagga actttcacct tcaagtgaat ctggactttg
    14341 ctgaattttg ttataaagtc ctgatggcgt aggttcttcg tcgatatcaa aaattttggc
    14401 atcttcgccc agcgtattgt gaatcatgaa cattttttgt gctgcaattt ctcttgaatg
    14461 aacgagatct gatccttttt cggtggggaa aaaattaata atgtagagtt tatcaaaaac
    14521 ttttttactg atacgattga ttcttcctat gcgttgaatc acgcgtaccg gattccacgg
    14581 aatatcatag ttgattacca tgccggcacg attcaaattg aatccttccg aaattttatc
    14641 agtagttagc aaaatgttgt aatcatcagc ttgttgagtg gctgtggcat cgaaattttt
    14701 attgatttcc ttaattttgc tggccgggag attacctgct accaccaata cacgtccgtt
    14761 gaatgcctga gtgagcaagg gagccagata attgactgta tcggcatatt ccgaaaaaat
    14821 gataactttt cttttgggtt caaagaggtt ttcaggcgga gcgtcgagca ctgtttttac
    14881 ttcttcgatg aggcgttcgg ctttggggtc ttttgctacc agattcagtt tgtgcaattc
    14941 gccaagaatc tgctggaata atgcaatatc tgattgaata tcattgataa aatcggtttc
    15001 acgttcaaat tttttcagtt catacacttt atggttttta ggatattcat tatttttgat
    15061 tttttcagaa tattcttgca aataattttc aatttcgtcg gcatccaatt cataaatttt
    15121 ttccagcaaa ttcctgtcga gaatatattt atcggttttt tggataaatt ccagtacaat
    15181 ttgatggatg ttgatgaagt ttttcacgct ttgttcaaaa gcgccaaatg aactttcgaa
    15241 acgttttacc agcaatcgcc gcataaaatc gtacaagttg aattgttgca aatattggaa
    15301 attttcttct ttgcccagct cacctttttt tactttttgc aactcatatt caaaaggttt
    15361 gtaaatggct cctttaaaac gtcctccgtc atcaggcaag gcaaagtatt gttgaatgat
    15421 tttgtcgtaa aataccgatt gttccttgtt cagttcatag aaccattctt ttggagattc
    15481 tacaattgat agctcgttta cttcattttt gtaataagga tgattttgaa tgtccagccg
    15541 atttcggcga ataatcactg gttcaataac attgcgaatt tgttttgcca gaatttctgt
    15601 tcgttttttt attttagtcg aatcgactat cttttcgcca aataaatcag tgtaataaga
    15661 aagtgctctg tttcgctttt gccggtcaga cgattggaaa tatcgcttga tgtaagaaag
    15721 tttatcaaat tccgatttga atgttctgaa tttattttcc agatcatcat caagtgtaat
    15781 acttgatttt ttgggaacaa taaacagttt gagcaacgaa aatatatcgg ctggcttgtt
    15841 attgaatggt gtagcagtaa gtaagatgac aattttatcc cgacaaatat ttttcaggta
    15901 ctcgtaatct ttggtatctt catttctgaa tcgatgcgct tcgtcaataa ttaccacttc
    15961 gatatcgttg gttttcgaaa caaattccga aacgtgttca aggtcgccaa gtgaaaaagc
    16021 ttcccagcca agagaagcca tgtgaaattg ttcaagatat tttttccagc cttctgattt
    16081 ggctttatcg ccgataattc ccggaggtgc aatcacaatt ccacgttttt tcaattcaaa
    16141 agcaatactg caagcaataa cggttttacc cagtcccact acatctgcaa tgatcacgcc
    16201 gttgttattt tcaataatag aaagtgcttg ttgaacagca tctaactggt aacgataggg
    16261 tttgtatcca ttttcttcaa gcacttcggt aagccgtcgc gtgacttctt ttccacgaaa
    16321 tgtgtcaaga taatttttca agacaaaaag ataagcttca aaaggcgtaa tttctttcac
    16381 cagcgtttct tgttcaataa ctttgacaat tttttccttc aactcgtcgt tttcagtaat
    16441 ttttatggca ttattccaga gttcatcaaa ataactttct gcttcatcaa aaccataatc
    16501 gctaatttct acattaaatt ctgcttgcga cattaatccg gctgaagtca gattactgct
    16561 tcccgtgata aaaagtctgt ttcgcccgat ttgatcttct tctaatttaa aaatgtagag
    16621 cttggcatga ttgggttcgc gtgtttttct gagaataatt tcgtcgtttt ttatcttttg
    16681 aaggaaaaat ttaacctgct cataaaaatc tttatggtca aaaacatctg aatttatgga
    16741 ttttcggata gaagtaaaaa agttttctat tcgttcatca tctgaaagtt ttttatcttt
    16801 ttcaccatat tctgtaagcc catgaatggt tttatccaca tttaagccga tcagcacttt
    16861 tagttttact ttatcgttcg attgcaagcc atcgtacaat tctcttaaac caaaaaaata
    16921 aaaaaatcca accagaaatt tcagttcttc acttttagaa agaagttcaa taagacgcgt
    16981 tctcagttgt ttttttgtac tgttagtaat aaaattgttc ataatctttt attaatcaat
    17041 aaaaaaactt taagactagg tggaaaactt ttttgaaaat taaaaaaaca ataaaataag
    17101 tttcttttta aaaagtatga caaatataca cattgaaaag caaagtaaag aaagttcttt
    17161 gaaaaataaa ttgtttcaaa aagttttata atttggattt acgtattgat tttgcttctc
    17221 taattttatt ttgaaatgcg acgaggcgtt aacaataagt gtgtattttc aatttttttg
    17281 aagtgaactt tttcaaaaaa tacgattttc ttgatgaaat attgttgttt ttttactgta
    17341 aaaatcaaac aaataaaata tatttgcaga tactaatatt ttttaattct tttgtttttt
    17401 atctacaaga gtattatgtt aaatgattaa aatttttcag taaaaataat ttttccaata
    17461 aagaaaaggt cttgattttt ttagtttatt tttaaaatca aaatattatg aataagtatt
    17521 tgcaacatga tccctggtgt attatagaag aaggttttca tatggacagc cagcttgctt
    17581 cagaaagtat ttttagttta ggtaacggac acatgggtca acgtggtaat tttgaggaat
    17641 attattctgg agaaaccatg tttggttctt atattgcagg aatttatttt cctgaacgcg
    17701 cagaacggag caactggaaa aacggttact ctgaaacaaa tgacaaaata gtcaatgctc
    17761 ctaactggat ttgtatcagt gttcgtttaa atgatgaacg attggattta ggttcgtggg
    17821 atattcacaa tttcaggcgt gttttaaata tgcgtgaagg ctatttggaa cgcacttttg
    17881 aagttacttc tcctaaaggt catcgcattc aagctacggt gaggcgattt ttaagcatgg
    17941 cagaaaatga agtcgctgtt attagttata ctgtgaaatc tatcaattac gaaggaagga
    18001 tctcgttttt acctttgatt gatggcaatg tcagaaatta ttataccaat tataatgaac
    18061 ctttctggaa tgtattgcaa actaaaaccg aacaggatgt ttctcattta tgggcacaaa
    18121 ctcgacgtct tgatttccat gtgtgtgcag cattgactta tgaattttat aaaaataata
    18181 tgcttttgaa tgttaatcct ataaaaattg aaaaagaaag agtagcaggg tttagtgtgg
    18241 gcaccgatat taaaaaagga gaaacagttt gcttgaaaaa atatgtgtct gtaatgagtt
    18301 ctttgaatca tccccgagaa gaacttacag acagagcatg taatttagct cgtgccgtta
    18361 agcaaaaagg ttgggataaa ttgtttgatg aacataaggc tgtttggaag gaaaaatggt
    18421 tgcaatccga tgtgattatt gaaaatgata tagcagctca acaagctatt cgatttaata
    18481 tttttcaatt aaatcaaagt tattcgggga atgatgatcg tttaaatatt agtccaaaag
    18541 gttttacagg cgaaaaattt ggaggcggaa ctcgctggga taccgaagca gtttgtgtgc
    18601 ctttttattt atcaacttct cctcaaaacg ttgcacgtaa tttattgctt tatcgttatc
    18661 gtcatttacc aaaagcaata caaaatgctg agaaacttgg ttttacgaat ggagctgctt
    18721 tatttcctgc tgctactttt aatggtagcg aatgtctaaa tgaatgggaa atttcttttg
    18781 aagaggtttt tcggaatggc attattgctt atgctatcta taattatctc cgatatacgg
    18841 acgacaatga ttttttagtc aattacggat taagaatttt aattgccatt gctcgtttct
    18901 gggctcaacg tgtcaatatt tctcatcaaa agaaaaaata tgtcttgtta ggtgtgacgg
    18961 gtccaaatac ttatgaaaac aatgttaata ataactggtt taccaatttt attgctgttt
    19021 ggtgtttaaa atttacaatg gaagccattg aatatgtgaa aaagaaagat tttagagctt
    19081 ttggtgattt gtcgcaaagt atcaattttt ccaatgaaga agtttttttg tggaaagata
    19141 ttacagaaaa tatgtatttc ccagaagata agaaactaaa tatttttctt caacaagatg
    19201 gtttttttga taaaacgttg gtaaaagcat ctgaaattcc agcttcgcaa ttgccactta
    19261 accaacattg gacgtgggat agaattttaa gatcaccgtt tattaagcaa gctgatgtag
    19321 tgttaggact ttatcttttt gaagatttgt tcgacaaaga aaccatacgc caaaattttg
    19381 atttttatga accttttacc gttcatgaat cttccttgtc gccttgtatt cattccattg
    19441 tagcttccag tattggcgaa attgaaaaag gttatcatct ttatttacga acagcacgtc
    19501 ttgatttgga cgattacaac gacgaagtag ctgatggatt gcatattaca agtatggctg
    19561 gtacatggtt gtctattgtt caaggttttg gtggtatgcg tgtcattgat aatcatttgc
    19621 attttaatcc tcatattcct gaaaagtggg aagcttatgc ttttaatgtt atttttagag
    19681 gaacacaatt aaaaattagg gtagagaaaa agaaagttat tcttcaacgc ataaaaggcg
    19741 aacctattga tttatatgtt tataataatc tgattcatct ttcagggaat gaatgtgagg
    19801 aaattgaaag aaaaaattga at
//
LOCUS       DAOSAS010000014        19775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_1937_length_19775_cov_12.629006, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000014 DAOSAS010000000
VERSION     DAOSAS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19775
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1937_length_19775_cov_12.629006"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLR72_01295"
     CDS             complement(<1..561)
                     /locus_tag="PLR72_01295"
                     /inference="COORDINATES: protein motif:HMM:NF023074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3256 family protein"
                     /protein_id="HOK35879.1"
                     /translation="MNNLKKILTISILFLQTSLYAQKIENLYVHMPDRLDPVLNSKQR
                     HELIEYAKVGQSDTVTTRNGSKAYLLFLDTLNAQIKIKNTSSSTFEMKLLHFANGQPF
                     VGIIRTVCAPVCHSMIQFCDTTWNKIPLQFNYPKAIDWLNVKNIQDSTIEVAHLQKIL
                     ENTFITLTFEQGSNRLIATNNSLDFLS"
     gene            complement(554..>4565)
                     /locus_tag="PLR72_01300"
     CDS             complement(554..>4565)
                     /locus_tag="PLR72_01300"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOK35880.1"
                     /translation="NSSFRYTSQKNYQPLPKDKFNIHQFQFDHINGEVAVNVAGKDNV
                     NGSIKKFSGIEKSGFQLKNISSNFHYTNQSLQFPFLDIELPNSMIHLDSLQFDYPKTE
                     DLKNFNDKVRLKIPLHSSYVSPSDFSAFVPSFKNINGKFFIDAETSGKLSSMHFKNIQ
                     IKYGDSFSFTANVDLNGLPDFNETFIYADINDLHFKRADLQDFIADLTERPVILPSAF
                     NQIGTIRYKGNISGFFNNLVAYGKLKSDVGEISTDVLFRFGQSFKEFSYNGTLKTPGL
                     KLDKILNNPQFGNLIFNINTQGTKKENNDFQGIIEASVPEFQWKSYSYNNMIFNGNYS
                     NSGFNGTFEMVDDNIAAYFNGIIDLTQQSPTFDFHFKLSHADLNALNLVKKYPLALLS
                     FDSKINMVGNPVDQLNGTIEVDNIQFTNQHKTLNVQKIQLTSRQEPDFTSLIISSDFV
                     NGYMNGRYNYNTLPRTFKKLIQYYLPSLSFDYNSSASQKPNYFNVDLQISNIKELTEV
                     LQIPYTLKENSIIKGSVDERNNTVNLTADFPFFQKGKSEFKNTTLSLQTENQQLQLTT
                     NTQLKQKNGFMNIYLKALAGENVVSTLFAWQNTDSIKNAGEFQTVTQLRKENNKTFAQ
                     LSILPAQVIISNSLWNIHPSTLDFKPDGSICVHNFLFDNDQSQYIQINGIASKNQDDA
                     LEFKLKGVDFSFIMNDLVKLKALSLGGFFTGEATLYSALYQPFFEAKMFVKDYQMNHV
                     ILGDADLLSYWDKENKQIYSEATIVDKKDTVATIEGAYIPGKDSIDFIYDLHRVSVEM
                     LKPYLGSVVQNVKGLATGKLRMFGPTKKMGFHGDLFMEKAQVTMFFTNTTYFFNDTIH
                     MRRYSTEFKNITIYDEDGNRGTLNGKLIHDGMFGDLTYNVTVTAKNLLSMNTHPEDNP
                     IFYGKIYADGVVHVFGDEKVTNLFVNAVSKPHTECNINIPTTATASESNFVNFVKKET
                     DIEKEKKIETTIKPESNSNTKVNIQIEVTPDAEIRLIVDPQAGDMIEAKGSGNLRFEF
                     DNYSDIKVFGTYIIEKGNYLLTLQNVIRKEFKIVEGSTMSWTGNPYNAQINVRGIYSL
                     TASLRDLLDESQLMSIPRTTVPVNCILLMTGNLMSPTINFDIDLPSSDESVKQLVKSI
                     INTKEMMARQIVYLLIFNKFYNPYLQQTANAPVGIGTSEAISFLTSTVSAQLNNLLSQ
                     MINSNIISIGIDYQQSEISSSNVQAQVLIQPNDRLIINGNLGYRNDNINLSTNTNKFI
                     GDVDIEWLLTENGKFRLKAYNHTIDRYQLRAAKNTQGIGFMYKADFESFEDLFKYYWQ
                     KITFKKDKSSNEKNNE"
     assembly_gap    4566..4665
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4666..5112)
                     /locus_tag="PLR72_01305"
     CDS             complement(<4666..5112)
                     /locus_tag="PLR72_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35881.1"
                     /translation="MVFILLQNSKVQNSITQTLVKELSQKLNTKVSVGEVHIKLLNRL
                     VINDFYIEDQQKDTLLYVNKVNAHFNLWNLFNKKIIIHSAEFQQLFGNLVFDQQGKPN
                     FDFIIQSFKKPKTTQPQKFEFKIQRFELKNSSFRYTSQKNYQPLPKD"
     gene            5319..6350
                     /gene="tsaD"
                     /locus_tag="PLR72_01310"
     CDS             5319..6350
                     /gene="tsaD"
                     /locus_tag="PLR72_01310"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOK35882.1"
                     /translation="MDHITILGIESSCDDTSAAVVRDGILLSNVIANQKVHASFGGVV
                     PELASRAHQQNIIPVVHEALKQANIDKKELSAIAFTRGPGLLGSLLVGTSFAKGLALA
                     LDIPIIDVNHLQAHVLAHFIKQSENDNTPQPPFPFLCLLVSGGNSQIVLVKAYNKMEI
                     IGQTIDDAAGEAFDKCAKILGLPYPGGPYIDQLAKEGNPHAFKLNKPHIKGYNYSFSG
                     LKTSFLYLVKDKIKENPQFIQENLNDLCASLQNAIIGVLMSKLKKAAQDLDISDIAVS
                     GGVSANSALRQTFYDYAKKYGWRVYIPPFAFTTDNAAMIAITGYFKYLDKDFCSFDEV
                     PFARVSNTF"
     gene            6357..6947
                     /locus_tag="PLR72_01315"
     CDS             6357..6947
                     /locus_tag="PLR72_01315"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOK35883.1"
                     /translation="MKKTFSYIKNKLAPYNGIIYFIVILLVCHFVWKFLVKGDETDQF
                     VSFLGIDLTPFFNFMVNHTAHATKAVLNFLGVDIQLYLNYVMKLSNDGFGIRIIWACT
                     GIKQAYIFFCIIAFYRGPWLKKLWYIPAGLVIIYLYNIFRISLTVALVQNHPSWFTFL
                     HEYVLKYLFYVIIFGMWVLWEEKFVVKKSVKIISKN"
     gene            complement(7259..9187)
                     /locus_tag="PLR72_01320"
     CDS             complement(7259..9187)
                     /locus_tag="PLR72_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK35884.1"
                     /translation="MNKKSNLCTLVFLFTFNLFFYAQIPNDPINIQLSDVEVVRQRNK
                     LYSELGRVLTVINKEEINRMPVHSIDELLDQISGLDIRQRGTFGVQADISVHGGSFDQ
                     VLVLLNGVNITDPQTGHFNLDIPLNFNEIDRIEILQGSAARLLGPNAFSGAINIITNQ
                     NKKQNQLKAELSAGSNEYSTQNISTDFNIKNVTNFISFSHKSSDGYIENTDFDITNIF
                     LHSTLRTVATGKFDFQLAAQQKGFGANEFYSLAYPHQYEKTQTYFTSLSWNLNKGKLS
                     YLAQIYWRKHHDRFELFRNYEDAPSWYKNHNYHLTDIKGAKANANYIDNWGKITLGVE
                     LRNEHIYSTVLGEPMKKTVTDPFEHNIEFTHEGKRFHSTFLMDYSKDFQHWYCSTGIA
                     TTHSSSFGQNTFGGFDIAFAFTDEMRIFFSANSAIRLPTFTDLYYQSATQISNPNLKP
                     ETSKNLELGIKSNQKNLQWNICTYYRAGKNIIDWIKHPDSTKWISSNLIHINAFGTDF
                     HIDYQFIGSFLKSASFSYSYLYQDKNAEQFDSKYALDYLKHKANLKLEHPFWKNLTGI
                     WAISYFDRAGNYTAFQTGKLVNYKPYLLADFQINWAKSQYEIYSNLNNLLNVHYEDYG
                     GIAQPGFTFNVGFRWKFL"
     gene            complement(9201..9587)
                     /locus_tag="PLR72_01325"
     CDS             complement(9201..9587)
                     /locus_tag="PLR72_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35885.1"
                     /translation="MKIHHTFSFKKNVRFRRWCKKGYAVFCSLTHQVTIGVLAASICD
                     KSTLKSKKNILTDIPSEKNEFVSFIEKGPLINEDFLLLFNNSITINFSIVSSNDVILP
                     LANQLINFTQRLRKVPSFFNRFFINS"
     gene            complement(9642..11024)
                     /locus_tag="PLR72_01330"
     CDS             complement(9642..11024)
                     /locus_tag="PLR72_01330"
                     /inference="COORDINATES: protein motif:HMM:NF039698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6242 domain-containing protein"
                     /protein_id="HOK35886.1"
                     /translation="MPSPYLKLSIFCVAIIFLNSCLGDDYVTDTSNNPCFVSLSFLKN
                     NSVPNLENAVFTLVFDETIHDSIIVNLDSLPYQTRIDSVYAVFSFKSSSACYLIFDND
                     SLVTYSSGNPIDFTKVSQIKNIASDGVTERTYYIKVNVHQVDPELYVWKKLIDNVYSF
                     TAQDQKAIYFKGKIYFFVSSNAGNFLYNSTNGTSWTEYTINGLPPLATFQDIQIFNDT
                     TLFLTQNNSNLYFSTDGINWSLYQYADFQFSSLLFVMNNQLWAIVNTINDGKYRSASS
                     NDGSYWQLNNEIPANFPISNFTSISFYSRTKTPKALVINGTSVDNQPLGTRWSTENGS
                     YWVDFSIEDKSLDSLDQGAPVIFYDDKLFILGRNKEKTEKNYFRQSIDEGLSWQIPDS
                     NYNYLPEDFQARHYSSFFVFNPRPYDKTDKKEQIEASNRIYIIGGKNSSGWFSDVWTG
                     KVNKKNFLIQ"
     gene            complement(11127..11888)
                     /locus_tag="PLR72_01335"
     CDS             complement(11127..11888)
                     /locus_tag="PLR72_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOK35887.1"
                     /translation="MKIISYNINGLRAALTKNFTDWLQAENPDVLCLQEIKAQPDQID
                     TWLFAELGYITYIHSAEKKGYSGVATLTKIPPDNVVMGMQNPVYDAEGRVLRTDFEDI
                     SILNVYIPSGSSGGERQQFKMQFLEDFLPFIKELLKLRPNLVVCGDFNICHKPIDINH
                     PELQEGVSGFLPEEREWMDRFQEAGFVDSFRVFDQSPGKYTWWSFRGGARSRNAGWRI
                     DYHWISKPLLPRLKSAKILSEVFHSDHCPVMIELE"
     gene            complement(11923..12693)
                     /locus_tag="PLR72_01340"
     CDS             complement(11923..12693)
                     /locus_tag="PLR72_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK35888.1"
                     /translation="MIEVKNLYKSFEGVPALTDISAVFETGIVNMIIGQSGSGKTVLM
                     KSIIGLHRIDKGRIEYDGRNLPDMTMKEIRILRREVGMLFQGSALFDSMTVLENVMYP
                     LEMFSSMSKKEMIQRAHFCLERVNLEKSSFDLYPSEISGGMQKRAAIARAIALRPKYL
                     FCDEPNSGLDPITSLIIDDLIYKITHEFNITTIINSHDMNSVIGIGEKIIFINNGKKA
                     WEGTKNDIFKSDNEELNDFVFASEVFKQLKAMKINNKK"
     gene            complement(12775..13515)
                     /locus_tag="PLR72_01345"
     CDS             complement(12775..13515)
                     /locus_tag="PLR72_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK35889.1"
                     /translation="MNIFQDTGRYCILMEHVFIKPDRWKMFFRQFRNELDKLGLQSLP
                     IVLIISVFIGAVLTIQTKLNSENPLLPSYTTGLVTRDTLLLEFSSTIMCLILAGKVGS
                     NIASEIGTMRITEQIDALEIMGVNSANYLILPKIVAFVLMIPFLTIFSMAIGLIGGYF
                     VALFSDIITVAEYVTGIQYAFNAYYVFYSIVKSLVFAFLISSVSSYYGYYAYGGALDV
                     GKASTTAVVNSSILILFFNLLITNVMLK"
     gene            complement(13858..14280)
                     /gene="mscL"
                     /locus_tag="PLR72_01350"
     CDS             complement(13858..14280)
                     /gene="mscL"
                     /locus_tag="PLR72_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOK35890.1"
                     /translation="MKELLAELKAFLMRGNVVDMAVGIVIGAAFGKVVTSFVNDILMP
                     PISLLLGSTNFSDLKIVLKNAVVEGTNVVAPAITWNYGNFIQIIIDFLIVGTAIFFLI
                     KGMNQLSRKKEEAPAPAPEPTNEEKLLAEIRDLMKKEN"
     gene            14940..16136
                     /locus_tag="PLR72_01355"
     CDS             14940..16136
                     /locus_tag="PLR72_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOK35891.1"
                     /translation="MFQNKQVAKDIFYVGVNDRQKAKFENIIPLPFGVSYNSYLIIDD
                     KTALMDTVDFHQGEIFVDKIQSQLQGRKLDYLIINHMEPDHSGSIRLIRKYFPEVILV
                     GNSRTLKMIEGFYGEASCVKEIHDEEILSLGTHELQFFLVPMVHWPETMMTFDRTEKV
                     LFSGDAFGCFGALDGAVVDTKMNVGKYWDEMIRYYANIIGKYGSPVQKAIQKLSGVPF
                     ETICTTHGPVWKENIQKTINIYDQLSKYEGGNGIVIVYASMYGNTELMAETIAQGVAE
                     AGVKEITIHNASTTDASFILRDIFKYKGLIIGSPTYNNELFPEIEALLQKIELRQVKN
                     RIFGCFGSYTWAGAAVDRLSLFAEKMKWIMVDKGIEEKQALKDNQYKACLQLGLKMGE
                     MLIQSF"
     gene            16227..16457
                     /locus_tag="PLR72_01360"
     CDS             16227..16457
                     /locus_tag="PLR72_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOK35892.1"
                     /translation="MKKNNPSELSKINYLLIAIGFVIIVIGFFLMTGSSTEITFNPDI
                     FSFRRITLAPMISLFGFLFIIVAILYQPKKKE"
     gene            16458..17342
                     /locus_tag="PLR72_01365"
     CDS             16458..17342
                     /locus_tag="PLR72_01365"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOK35893.1"
                     /translation="MSFFQAILIAIVEGLTEFLPVSSTGHMIIVQSLLGIESTDFVKV
                     FTVNIQFGAILSVIVLYWKRFFKLNPLPALDKTFIESKKDFAKLKVYFQRFLYKYDFY
                     WKLLIGFLPTGIIGFLASDYIDTLLGEVWVVALMLLLGGILMIFIDQWLKYPSSNQEI
                     SWKRALEIGFFQCIAMIPGVSRSMATIVGGMATKLNRKNAAEFSFFLAVPTMAAASIF
                     KLIELLHTSNGSQVLTQNLSLLLVGNIVAFVVAMAAIKFFIDFLTKHGFKAFGYYRII
                     VGAFLLILIYLGIDLKIV"
     gene            17342..>17591
                     /locus_tag="PLR72_01370"
     CDS             17342..>17591
                     /locus_tag="PLR72_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase"
                     /protein_id="HOK35894.1"
                     /translation="MDVIAGEVFYVDKPLHWTSFNVVDRIRKKITKKLKIKNIKVGHA
                     GTLDPLATGVMIICTGKATKSIDLYQNQTKEYIATLKLG"
     assembly_gap    17592..17691
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17692..18207
                     /gene="truB"
                     /locus_tag="PLR72_01375"
     CDS             <17692..18207
                     /gene="truB"
                     /locus_tag="PLR72_01375"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOK35895.1"
                     /translation="IDLYQNQTKEYIATLKLGATTPSFDLELPIDKIYPTHHITLEMI
                     ENVLHNFLGEIDQIPPIYSAVKVDGERAYKYARKGEKVELKPKKLVINKLEVLEFNLP
                     LLKIRIECSKGTYIRALARDIGKTLGSGAHLVALQRTRIGEVRLEDCWQLNDLLQYLD
                     DELLNSVPFTK"
     gene            18448..19497
                     /gene="queA"
                     /locus_tag="PLR72_01380"
     CDS             18448..19497
                     /gene="queA"
                     /locus_tag="PLR72_01380"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOK35896.1"
                     /translation="MKLSQFKFKLPDELIAQYPAPHRDESRLMVVHRKTGEIEHKIFK
                     DVLDYFNENDLFVLNDTKVFPARLYGNKEKTGAQIEVFLLRELNPDLKLWDVLVEPAR
                     KIRIGNKLYFGNDDSLVAEVVDNTTSRGRTLRFLFDGTHEEFKKTLYSLGETPLPRYI
                     NRSVEKEDAERFQTIFAKNEGAVAVPSAGLHFSRELMKRMEIKGIEIAFLTSYMNLGN
                     FREIDVEDLTKHKMDSEQISVPEETAEKVNKAQENRQNICAVGATVMRALESVACTDG
                     KIKSFEGWTNKFIFPPYDFTVANALITNFHFPYSTFLMLTAAFGGYDLIMDTYQIAIE
                     EKYRFGAYGDAMLVI"
BASE COUNT         6644 a         3043 c         3212 g         6676 t
ORIGIN      
        1 acttaaaaaa tcaaggctgt tattggttgc tattaagcga ttgctacctt gttcaaaggt
       61 cagcgttata aaagtatttt caagaatttt ctgtaaatga gcgacttcta ttgttgagtc
      121 ctgaatattt tttacattga gccaatctat tgcttttgga taattaaact gcaagggtat
      181 tttattccat gttgtatcgc aaaactgaat catggaatga catacagggg cacaaacagt
      241 gcgtattatt cccacaaaag gttgtccatt tgcaaaatgt aaaagtttca tttcaaaagt
      301 actggaagaa gtatttttaa tttttatttg tgcattcaat gtatctaaaa acaacaaata
      361 agctttgctg ccatttcgag tggtaacagt atcactttga ccaacttttg catattcaat
      421 caactcatgc ctttgtttcg agttcaaaac aggatctaat cgatctggca tatgaacgta
      481 aagattctca atcttttgag cataaagtga ggtttgtaaa aataagatac tgatagtcag
      541 tatttttttt aagttattca ttgttttttt catttgatga tttgtccttt ttaaacgtga
      601 ttttttgcca gtaatatttg aataaatctt caaaactttc gaaatcggct ttgtacataa
      661 aaccaattcc ctgagtattt tttgctgctc tcaattgata acgatcaata gtatgattat
      721 aagctttcaa acgaaatttc ccattttcag tcaataacca ttcaatgtcc acatcaccaa
      781 taaatttatt tgtattggta cttaaattaa tattatcgtt tctgtaaccg agatttccat
      841 tgataatcaa tctgtcatta ggttgaatca gcacttgagc ctgcacgttg ctacttgaaa
      901 tttctgattg ttgataatcg atcccaatgg agatgatatt gctgttgatc atttgtgata
      961 gtaaattatt caattgagca cttactgtag aagttaaaaa tgatattgct tcgctggtgc
     1021 caatacctac cggtgcgtta gctgtttgtt gcaagtatgg attataaaac ttattaaaaa
     1081 tcagcagata aacaatctgg cgtgccatca tttctttggt attgatgata ctttttacca
     1141 attgttttac actttcatcg ctcgatggca aatcaatatc aaaattgata gttggcgaca
     1201 taagatttcc tgtcataagc agaatgcaat tgacaggcac cgttgttctt ggaatagaca
     1261 ttaattgcga ttcatctaac aaatcgcgta aagaggctgt caatgaataa atgcctcgta
     1321 catttatttg agcattgtac ggattacctg tccagctcat agtgctgcct tctactattt
     1381 taaattcttt gcgaatgaca ttttgcaagg ttaacaaata atttcctttt tcgataatat
     1441 aagtgccaaa tacttttata tcagaataat tatcaaattc aaatcttaaa tttccgctac
     1501 ctttggcttc aatcatatcg cctgcttgtg gatcaacaat taaacgtatt tctgcatctg
     1561 gagtaacttc aatctggata tttacttttg tattactgtt actttctggt tttatcgtcg
     1621 tttctatctt tttttctttc tcaatatctg tttccttttt tacaaaatta acaaaattac
     1681 tttcggaagc agtagcagtg gttggaatat taatattaca ttctgtgtgt ggtttagaaa
     1741 ccgcatttac aaataaattg gtaacttttt catcgccaaa gacatgtacc actccatcag
     1801 cataaatttt tccgtaaaaa attggattat cttcaggatg agtattcatc gacaaaagat
     1861 tttttgctgt taccgtgaca ttataggtta aatcgccaaa cattccatca tgtataagtt
     1921 ttccatttaa agttccacga ttaccgtctt cgtcatagat agtaatattt ttaaactccg
     1981 tagaatatcg cctcatgtgg atggtatcgt taaaaaaata agttgtatta gtaaaaaaca
     2041 tggttacctg ggctttttcc ataaataaat ctccgtgaaa ccccattttt ttcgtaggac
     2101 caaacattcg tagttttcct gtggcaagtc cttttacatt ttgaactacg cttcccagat
     2161 aaggttttaa catttcaaca gatacccgat gcaaatcata aataaaatca atagaatctt
     2221 ttcctggtat ataagcacct tcaattgtag ctacggtatc ttttttatcc acaatagtag
     2281 cttccgaata gatttgcttg ttttctttat cccaataact taataaatcg gcatcaccca
     2341 atattacatg attcatttga taatctttta caaacatttt tgcctcaaaa aaaggctgat
     2401 acaaagcact atacagggta gcttctcctg taaaaaaacc acctaaactc aatgctttca
     2461 atttaaccaa atcattcata ataaatgaaa aatcaacacc tttgagttta aattccaggg
     2521 cgtcgtcctg atttttcgaa gcaatgccat ttatctgaat atattgactt tgatcattat
     2581 caaaaagaaa attatgaaca caaatagagc catcaggctt aaaatcaagg gttgaaggat
     2641 gaatattcca cagtgaattt gaaatgatga cctgggcagg taaaatagac aattgagcaa
     2701 atgttttatt attctctttt cggagttgag taacggtttg aaattcacca gcatttttaa
     2761 tggaatctgt attttgccaa gcaaataagg tacttacaac attttctcct gccaaagctt
     2821 tcagatatat attcataaaa ccatttttct gcttcaattg agtgttcgta gtcaactgta
     2881 attgttggtt ctcagtttga agtgaaaggg tagtattttt aaattcgctt ttcccttttt
     2941 gaaagaatgg gaaatcagca gttaaattaa cagtattatt tctctcatct acagaacctt
     3001 tgataatcga attttctttt aaggtataag gaatttgtaa aacctccgtg agctctttga
     3061 tatttgaaat ttgtaaatct acattgaaat aattgggttt ctgggaggca gaagaattat
     3121 aatcaaaaga taaagaaggt aaataatact gtatcaattt cttaaaagtt cgaggaagtg
     3181 tgttgtaatt atatcttcca ttcatataac catttacaaa atcggaagaa atgataagac
     3241 ttgtaaaatc tggttcttga cgagaagtta attgaatttt ttggacattc aaagttttat
     3301 gctgattggt gaattgaata ttatcgactt cgatagtgcc attaagttga tccacagggt
     3361 tgcctaccat attgattttg ctatcgaaag ataataaagc gagaggatat ttttttacca
     3421 gattcaaggc atttagatct gcatgggaga gtttaaaatg aaaatcaaaa gtaggggatt
     3481 gctgagttaa atctattata ccattaaaat aagctgcaat attatcatca accatttcaa
     3541 aagtgccatt aaaacctgaa ttactgtaat ttccattgaa aatcatattg ttataagaat
     3601 agctcttcca ttgaaattct gggacagaag cctcaataat tccttgaaaa tcattgtttt
     3661 cttttttagt tccttgagta ttaatattaa aaattaaatt accaaattgt ggattgttta
     3721 aaattttatc taatttcaaa cctggagttt tgagagtacc attataggaa aattctttga
     3781 aactttgacc aaatcgaaac aaaacatctg ttgaaatttc tccgacatcc gactttaatt
     3841 ttccgtaagc aaccagatta ttaaaaaatc cagaaatatt tcctttataa cgaatggtgc
     3901 caatctgatt aaaagcagac ggtaaaataa cggggcgttc cgttaaatca gcaataaaat
     3961 cttgtaaatc agctctttta aaatgtaaat cattaatatc agcataaatg aaagtttcat
     4021 taaaatctgg aagtccattt aaatcaacat tggctgtaaa cgaaaacgaa tctccatatt
     4081 taatctgaat atttttaaaa tgcatgctgg aaagtttccc acttgtttct gcatcgataa
     4141 aaaactttcc attgatattt ttaaaagaag gtacaaaagc agaaaaatca gaagggctca
     4201 catacgaaga atgaagcgga attttcaaac gaactttatc gttaaaattt ttcaaatcct
     4261 ctgtttttgg ataatcaaac tgcaagctgt ccagatgaat catagaattt ggaagctcaa
     4321 tgtcaagaaa aggaaattgc aatgattggt tggtataatg aaaatttgaa gatatgtttt
     4381 ttaattgaaa gccagacttt tcaataccac tgaatttctt tatcgaacca ttgacattat
     4441 ctttacctgc aacatttact gcaacttcac cattgatatg gtcaaactga aactgatgaa
     4501 tattaaactt atcttttggc aaaggttgat aatttttttg gctggtataa cgaaaggaag
     4561 aatttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatctt ttggcaaagg
     4681 ttgataattt ttttggctgg tataacgaaa ggaagaattt tttagttcaa aacgctgtat
     4741 cttaaattca aatttttggg gctgagtagt ttttggcttt ttaaaagatt gaataataaa
     4801 atcaaaattg ggttttcctt gctgatcgaa aacgaggttg ccaaataatt gttgaaattc
     4861 agcagaatga ataatgattt ttttattaaa taaattccat aaattaaaat gggcgttgac
     4921 tttatttaca tacaaaagag tatctttttg ctgatcttcg atataaaaat cattgataac
     4981 aagacgatta agcaatttta tatgaacttc accaactgaa actttcgtat ttaatttttg
     5041 agataattct ttaacaagcg tttgggtaat agaattttgt accttgctat tttgcaacaa
     5101 gatgaatacc aatgctaaaa aaataaggat gccagcaatg gtatagctta ttatttttat
     5161 tgtttttttt atacctttga aattttattt acaaaagtaa aaaaattatt tcctatttcc
     5221 agtaatctaa gctggcaaaa tagtagcttt tccaaatatt taattgcttt tgcagttttt
     5281 atatgagatt gctttttttg taaaaaattg ttagagaaat ggatcatatc actatattag
     5341 gcattgaatc ttcgtgcgat gatacttcag ccgctgttgt acgcgatggt atattgctgt
     5401 cgaacgtaat tgctaatcaa aaagtacatg ccagttttgg tggcgtagtt ccagaacttg
     5461 cttctagggc tcatcaacaa aacattattc ctgtcgtaca cgaagcatta aaacaagcca
     5521 atattgataa aaaagaactg agtgccattg cttttacgcg tggacctggt ctgttagggt
     5581 cgcttctggt gggcacttca tttgccaaag ggcttgcgct ggctcttgat atccctatca
     5641 ttgatgttaa ccatttacaa gcacatgtgt tggcacattt tatcaaacaa agtgaaaatg
     5701 ataacacacc acaacctcct ttcccgtttt tatgtttgct tgtgtcaggt ggcaattcgc
     5761 agattgttct tgtaaaagct tacaacaaaa tggaaattat cggacaaaca attgacgatg
     5821 ctgccggaga agctttcgac aaatgtgcaa aaatattggg gttaccttat cctggtggtc
     5881 catatatcga tcaacttgcc aaagaaggga atccacatgc ttttaagtta aataagcctc
     5941 atattaaagg ttataattat agttttagtg ggttaaaaac ttcttttttg tatttggtaa
     6001 aagataaaat aaaggaaaat cctcagttta ttcaagaaaa tcttaatgat ttatgtgctt
     6061 cactgcaaaa cgctatcatt ggagtactca tgtcaaaact aaagaaagct gcacaagatt
     6121 tagatatttc agatatagca gtttcaggcg gcgtatcagc taactcggct ttgcgacaaa
     6181 cattttacga ttatgcaaaa aaatatggct ggagagttta tatccctcct tttgctttta
     6241 ctaccgataa tgctgcaatg attgctataa cgggctattt caaatatctc gacaaggatt
     6301 tttgttcatt cgatgaagtt ccatttgcac gagtttccaa tactttctga ttttcaatga
     6361 aaaaaacttt ttcgtatatt aagaataaat tagctcctta taatggcatc atttatttca
     6421 tagtaatttt acttgtttgc cattttgtat ggaaattttt agtaaaaggt gatgaaactg
     6481 accaatttgt ctctttttta ggtatagacc ttactccctt tttcaacttt atggttaacc
     6541 acacagctca tgcaactaaa gctgttctta attttttggg agttgacatt caactctatc
     6601 taaactatgt gatgaaacta agtaacgatg gatttggtat cagaatcatc tgggcatgta
     6661 ccggaataaa acaagcttac atttttttct gcatcattgc cttttatcga ggtccgtggt
     6721 taaaaaaact ctggtacata cctgctggac ttgttatcat atatctttat aatattttta
     6781 gaatcagttt aaccgttgca ctagttcaaa accatccttc gtggtttact tttttacacg
     6841 aatatgtatt aaaatatctg ttttatgtca ttatatttgg catgtgggta ttgtgggaag
     6901 aaaaatttgt tgttaaaaaa tcggtcaaaa tcatttctaa aaactaatcc agcaacttta
     6961 ttatcaataa aaaattgata ataagtaaaa ataatcagtt ttttaatcac atttgtgaat
     7021 agctaaattg aattcattcc taagtaagaa atttcatgtt atacactaat tcatcaattt
     7081 atacgtaata gaagaaaaaa accgctataa tgtttatata gagtttttta taatttcgca
     7141 attacgtatt tacgtgatta actataaaat tctttccaac aaaatatcct ctttgagcat
     7201 gataccaaat tgcaattata aatacaattt agagtttaaa ttaatgacaa atttggacct
     7261 ataaaaattt ccatcgaaat cctacattaa aagtaaatcc tggttgagca attccaccat
     7321 aatcttcata atgaacattt aataaattat tcaggttgga gtaaatttca tattgacttt
     7381 ttgcccaatt aatttgaaaa tctgctaata aataaggttt atagttcact aatttccctg
     7441 tttgaaaggc ggtataattt cctgctctgt caaaataaga gattgcccat attcctgtaa
     7501 gatttttcca gaaaggatgt tctaatttca agtttgcttt gtgtttgagg taatccagag
     7561 catattttga atcaaattgc tcggcgtttt tatcttgata caaataagaa tatgaaaaac
     7621 ttgcactttt aaggaatgag ccaataaatt gataatcaat gtgaaaatct gttccaaaag
     7681 cattgatgtg aattaaattg gaacttatcc attttgtcga atcgggatgt tttatccagt
     7741 caataatatt tttacctgct cgataatagg tacaaatatt ccattgtaga tttttttgat
     7801 tcgatttaat tcccaattcc agattttttg aagtttctgg tttcagattt ggattgctta
     7861 tttgcgttgc actttgataa tataaatcgg taaaagttgg tagacgaatg gcagagttag
     7921 ctgaaaagaa aatacgcatt tcgtcagtaa aagcgaaagc tatatcaaaa ccaccaaaag
     7981 tattttgtcc aaatgaagaa gaatgagtag ttgctatacc tgttgaacaa taccagtgtt
     8041 ggaaatcttt tgaataatcc attaaaaatg ttgaatggaa acgtttccct tcatgagtga
     8101 attcaatatt atgttcaaac ggatcagtaa ctgttttttt cataggttct cccaaaacgg
     8161 tactataaat gtgttcattt ctgagctcta caccaagtgt gatttttccc caattatcaa
     8221 tatagttagc attggctttt gctcctttaa tatctgtcag atgataatta tgatttttgt
     8281 accaggaagg tgcatcctca taatttcgga ataactcaaa acgatcgtga tgttttcgcc
     8341 aatagatttg agccagataa ctgagttttc ctttgtttaa attccaggaa agtgatgtaa
     8401 aataagtttg tgttttttca tattggtgag gataagccaa agaataaaat tcattggcac
     8461 caaaaccttt ttgttgagca gccaattgaa agtcaaattt tccagttgct actgttctta
     8521 atgtagaatg aagaaagata ttggttatat caaaatcggt attttctata taaccatcac
     8581 tacttttgtg ggaaaaagaa ataaaattag taacattttt aatattaaaa tcagtagaaa
     8641 tattttgagt tgaatattca ttgctaccag ccgataattc agctttgagt tgattttgct
     8701 ttttgttttg gttggttata atgttgatag ccccactaaa agcattcggt cccagtagtc
     8761 gtgctgcaga gccttgtaaa atttctattc tatctatttc attgaaattt agtggaatat
     8821 ccagattaaa atgtcctgtt tgtgggtcgg taatgttaac tccatttaaa agcactaaaa
     8881 cttgatcaaa tgagccgcca tgaacagaaa tatcagcttg tacgccaaaa gtcccgcgtt
     8941 gacgaatatc caagccagaa atctgatcaa gaagttcatc gatactgtgt acaggcattc
     9001 gattgatttc ttctttgtta ataacggtaa gcactcgtcc cagctcggaa tataatttgt
     9061 tgcgttgcct aactacttcc acgtcagaaa gttgaatatt tattggatca ttgggaattt
     9121 gagcataaaa aaacaagtta aaagtaaaca aaaatacaag agtacataaa tttgattttt
     9181 tgttcattgg aataattttt ttaagaatta ataaaaaaac ggttgaaaaa agatggaact
     9241 ttcctcaacc gttgggtaaa attaattaat tgatttgcca atggcaaaat aacgtcatta
     9301 gaagatacta tcgaaaagtt aatagtaata gaattattga atagaagtaa aaaatcttca
     9361 tttattaaag gtcctttttc aataaagctg acaaattcat ttttttctga cggaatatca
     9421 gtcaaaatat tttttttaga ttttaaagtg gatttgtcgc aaatagaagc agcaagaact
     9481 ccaatggtaa cctgatgggt gaggctacaa aaaacggcat aacccttttt gcaccagcgg
     9541 cgaaaacgaa catttttttt aaatgaaaaa gtgtggtgta ttttcattct tcggcaaaaa
     9601 gggatagatc tttaaaattc attgaacttt tacagattca gttattgaat aagaaagttc
     9661 tttttattta cttttcctgt ccacacatca gaaaaccaac cggatgaatt ttttccacca
     9721 ataatgtaaa ttcggttaga ggcttcgatt tgttcctttt tatctgtttt atcataagga
     9781 cgtggattga atacaaaaaa tgaagaataa tggcgagctt ggaaatcttc gggtaagtaa
     9841 ttataattac tatcaggaat ttgccagctt aatccttcat caatagattg acgaaaataa
     9901 tttttttcag ttttttcttt gtttctaccc agtatgaaca acttgtcatc ataaaagata
     9961 acgggagctc cttgatcgag agaatccaac gatttgtctt ctatactaaa atcaacccaa
    10021 taagaaccat tttcagtact ccatctggtt cccaatggtt gattatcaac cgatgttcca
    10081 tttattacca gtgcttttgg cgttttagtt cgcgaataaa aggagatgga tgtaaaattg
    10141 ctgatgggga agtttgcagg gatttcgtta tttaactgcc aataacttcc atcattggac
    10201 gaagcagagc gatatttgcc atcattaatg gtattaacaa tagcccataa ctggttattc
    10261 attacaaaaa gtaaagaaga gaattgaaaa tcggcatatt gatataggga ccaatttatt
    10321 ccatctgttg aaaaataaag attggaatta ttttgtgtca ggaaaagtgt tgtatcatta
    10381 aaaatctgga tatcttgaaa agtagccaaa ggaggcaaac cattgatggt atactcagtc
    10441 catgaagtgc cattggtaga attatacaaa aaatttccag catttgaact gacaaagaaa
    10501 taaattttac ccttgaaata aatggctttt tgatcttgtg ccgtaaaaga ataaacatta
    10561 tcaataagtt ttttccatac atataattca ggatcaactt gatgaacatt tactttgata
    10621 taataagtac gttctgtaac accatcggaa gcgatatttt taatttgaga cacttttgtg
    10681 aaatcaattg gatttccgct ggaatatgtt accagcgaat cattatcgaa aattaaataa
    10741 catgcggaag aacttttaaa ggaaaatacg gcataaacac tatcaatacg tgtctgataa
    10801 ggaagtgaat caagatttac aataatagaa tcatgaatcg tttcgtcaaa caccaatgta
    10861 aaaactgcat tttccaggtt aggtacgcta ttatttttta aaaatgaaag agaaacaaaa
    10921 caaggattat tagaagtatc tgtcacataa tcatctccta aacaagaatt taagaaaatg
    10981 atcgctacac aaaaaatgga caactttaag taaggagatg gcataatatc aatatttatt
    11041 ttctttggac aaaattaaaa aaacatttta ttgaagtatt ttctaaggac aaagttttat
    11101 tattttttag ggaaatgttt tatattttat tcaagttcaa tcattaccgg acaatggtcg
    11161 gaatgaaata cttctgaaag tatttttgca gatttcagcc taggcaataa aggtttgcta
    11221 atccaatgat aatcaattct ccagcctgca tttcgagagc gagcacctcc acgaaaactc
    11281 caccaggtat attttcctgg actttgatca aacactctga acgagtccac aaaaccagct
    11341 tcttgaaagc gatccatcca ttcacgctct tcgggcaaaa agcctgatac accttcctga
    11401 agttctggat gattaatatc aataggttta tgacagatat tgaaatcacc acacaccaca
    11461 agattgggac gaagtttcaa taattccttt ataaaaggta aaaaatcttc taaaaattgc
    11521 attttaaatt gctgtcgttc gccaccagaa gagccagaag gaatataaac gttcaaaatg
    11581 gaaatatctt caaaatctgt tctgagtact cttccttctg catcataaac aggattttgc
    11641 attcccatca ctacattatc aggtggaatt ttagtaaggg tcgcaacacc gctatatcct
    11701 tttttttcgg cagaatgaat gtaagtgata tatcccaatt cagcgaaaag ccaggtatca
    11761 atctgatcag gttgggcttt tatttcttgt aaacaaagca catcaggatt ttcagcttgt
    11821 aaccagtcgg taaaattttt cgtgagtgct gcacgcaaac catttatatt gtaagaaata
    11881 attttcattg aaatttatta aaaaaaatag caaatctgtt ttttattttt tgttgttgat
    11941 tttcatagcc tttagttgtt tgaatacttc agatgcaaag acaaaatcat tcaactcttc
    12001 gttatcactt ttgaaaatat cattttttgt accttcccaa gcttttttcc cattatttat
    12061 aaaaataatt ttttctccaa ttccaattac tgagttcatg tcgtgggaat tgataattgt
    12121 agttatgtta aattcatgtg taattttata aatcaaatca tcaataataa gagaagtaat
    12181 ggggtcaagc ccagaatttg gttcatcaca gaatagatat ttaggtctta aagcaatagc
    12241 tctggcaatg gcagctcttt tttgcattcc gccactgatt tccgaaggat ataaatcaaa
    12301 agaacttttt tccaaattta ctcgttcgag gcaaaaatga gctcgttgta tcatttcttt
    12361 ttttgacatg gaagaaaaca tttcaagtgg gtacattaca ttttccaaaa cagtcattga
    12421 atcgaacaaa gctgatcctt gaaatagcat tcctacctca cgtcgtagaa ttcgtatctc
    12481 cttcatggtc atatcaggta aatttcttcc atcatattca attcgtcctt tatcgatacg
    12541 gtgtaagcca ataatacttt tcattaaaac cgttttaccc gaaccgcttt gcccaataat
    12601 catattaaca attccagttt cgaaaaccgc agaaatatca gttaaagcag gaactccctc
    12661 aaaagattta tacagatttt tgacttcaat catattttat agttaatcat aaaaatattt
    12721 ttgctttttc aaataacaca atgaattttt aaaaaattgt ttctggcaaa aatattattt
    12781 caacatcaca tttgtaatca ataagttaaa aaataatatc aatatgctgc tgttcactac
    12841 ggctgtagtg cttgcttttc caacgtccaa tgctccgcca taagcataat agccatagta
    12901 agaagaaaca gaagaaataa gaaaagcaaa aaccaatgat ttgactatgg aatagaaaac
    12961 ataataagca ttaaaagcat attgaattcc tgtaacatat tcagccactg taataatgtc
    13021 actgaaaagt gcaacaaaat agcctccaat taaacctatc gccatactga aaatggtaag
    13081 aaaaggaatc ataagaacaa aagctacaat tttaggaagg ataagataat tggcagaatt
    13141 tacacccata atttccaaag catcaatttg ttcggtaatt cgcatggttc caatttctga
    13201 ggcaatatta gaaccgacct tcccagccag aataaggcac ataatagtag aagaaaattc
    13261 taacaacaag gtatcacgcg taactaaacc agtagtataa gaaggtaata atggattttc
    13321 cgaattcaat ttagtttgaa tagttaaaac agctccaata aagactgaaa taattaaaac
    13381 aatgggtaat gattgtaatc ctaatttatc caattcattt cgaaactgac gaaaaaacat
    13441 tttccaacgg tcaggcttaa taaaaacatg ttccatcaat atacaatatc ttcctgtatc
    13501 ttgaaatata ttcatttctt atttttgtta ttaatgttaa atagagtttt ttaatcctta
    13561 tcttttgcaa agtttttata caacttaaaa aagcttctca aagaacagtc aaaaaatgtt
    13621 cttggagaaa gtttatgaat ggagattcct ttatcgaaaa gtgtacaaat atagttattt
    13681 tttgaaattt agtaaaaaac accttttgtt gaaaaaatag ctgaagttaa aaagaaaagc
    13741 ccagaaaaag tgagcaagaa atatatctta ctcattttct gcaattgtta attcaaatga
    13801 ttattttaaa agcagtaaca gcaattgctc atgaaaaaat tttcttgtaa acagcgatta
    13861 attctctttt ttcattaaat cgcgaatttc tgcgagcaac ttttcttcat tagttggttc
    13921 tggagcaggt gctggtgctt cttccttttt tcgacttaat tgattcatgc ctttaatcaa
    13981 aaagaaaata gctgtcccaa caataagaaa atcaataata atttggataa aattcccata
    14041 attccaggta atagctggtg caacaacatt tgtaccttct actaccgcat tctttaatac
    14101 tattttcaaa tcagaaaaat ttgtacttcc aagaagtagc gaaattggcg gcataagaat
    14161 atcgttaaca aaagaagtta ctactttacc aaaagcagca ccaattacaa taccaactgc
    14221 catgtcaaca acattaccac gcattaaaaa tgcttttaat tctgctaata gttctttcat
    14281 aatgataata atttaatgtt aattttttta tttgcaaata aaagaaaaaa accaatgtgt
    14341 tcaataatta aagaaagaat tttaagacgt ttatagtaaa tagatttgca aaaatgggga
    14401 aggatagaat ttgtccgaca agtaaaaaac tgacaaatta gcgagatgca acgtgcgagg
    14461 tgcgagaatg caaattccga gtttggtgtt ttgaagtaga aatgctttgt gaagcgtcta
    14521 acgaggtacg aagttcaagt tttgagtttt gagcgaatga agaaaaaatt tctgtatccc
    14581 attgttcgac gtactctcca ggctacgtcg aaataaaaat aaaatctctt gattttaacc
    14641 gctgatataa ttaaacgctt tgctaaacca atatttgtca taaaaaacct tattaaaaac
    14701 ctataacctt cataacaagg aatgttccct atagcttaca accttattat cttatttatc
    14761 aggtcgtttt tcttttctct gaatgtaatt agcgacgcca tttagaatca ttacctccaa
    14821 tgtatatcaa aaaaggtgcg tagcattgat atttttgtag ccactttgta aaatttgttt
    14881 tatctttgta gtactatttt tacatattat caaaatcctg taaaaaagct tcaacaatga
    14941 tatttcagaa taaacaagta gcaaaagata ttttttacgt tggagtaaat gatcgccaga
    15001 aagcaaaatt tgaaaatata attccgttac cttttggagt ttcctataat tcttatttaa
    15061 ttattgatga taaaacggcc ttaatggata ctgttgattt tcatcaaggt gaaatattcg
    15121 ttgataagat tcaatctcaa ttgcaaggta gaaaacttga ttatttaatt attaaccaca
    15181 tggagcctga tcattcaggt tctattcgtt tgattcgcaa atatttccca gaagtcatat
    15241 tggttggtaa ttcaaggaca ttgaaaatga tagaaggatt ttatggtgaa gcctcttgcg
    15301 tgaaggaaat tcatgacgaa gaaatactat cattaggcac tcatgagctt cagtttttcc
    15361 ttgttccaat ggttcactgg cccgaaacaa tgatgacatt tgaccgaaca gaaaaagttt
    15421 tattttctgg cgatgctttt ggttgttttg gtgctttaga tggagctgtg gttgatacta
    15481 aaatgaatgt agggaaatat tgggatgaaa tgatacgtta ttatgcaaat attattggta
    15541 aatatggttc tcctgttcaa aaagctattc agaaattaag cggagttcct ttcgaaacaa
    15601 tttgcacgac gcatggacct gtttggaaag aaaatattca gaaaacgata aatatttatg
    15661 accaattaag taaatatgaa ggggggaacg ggattgtaat agtatatgct tcaatgtatg
    15721 ggaatactga actaatggct gaaaccattg ctcaaggtgt ggcagaagct ggagtaaaag
    15781 aaattaccat tcataacgct tccacaactg atgcgtcatt tattttaagg gatatattta
    15841 aatacaaggg attgatcata ggaagtccta cttataacaa cgaacttttt cctgaaattg
    15901 aagctttgct tcaaaaaata gagcttcgtc aagttaaaaa ccgtattttt ggttgttttg
    15961 gttcttatac atgggcagga gcagcagtag atcgtttgag ccttttcgct gaaaaaatga
    16021 aatggataat ggttgataag ggaatagaag aaaaacaggc tttgaaagac aatcaatata
    16081 aagcttgcct acagttaggg ctcaaaatgg gtgaaatgtt aatacaatct ttttaacaag
    16141 aaaaaaagga aaataatttt ttaaaatatt ctttagataa aggaaattca tatcattttt
    16201 ttaatctttt atcaaattaa aaagaaatga aaaaaaataa tccatcagaa cttagtaaaa
    16261 ttaattatct ccttattgca ataggttttg tcattattgt aattggtttt ttccttatga
    16321 caggttcttc taccgaaatt acgtttaatc ctgatatttt tagttttaga agaataacac
    16381 tcgcacccat gatttctctt tttggtttct tattcattat tgtagccatt ctgtatcaac
    16441 caaaaaagaa agagtaaatg agtttttttc aggccatttt aatagccatt gtcgaagggc
    16501 tgacagaatt tttgccggtt tcttctacag ggcacatgat tattgtccaa agccttttgg
    16561 gcattgaaag tacagacttc gtaaaagttt ttactgtcaa cattcagttt ggagccattc
    16621 tgtcggttat agtactttac tggaaacgtt tttttaagct caaccctctt ccggctcttg
    16681 ataaaacatt tatcgaatca aaaaaagatt ttgctaaatt gaaggtttat tttcaacgtt
    16741 ttttatataa atacgatttt tattggaaac ttttaattgg ttttttgcca acaggtataa
    16801 ttggattctt ggcaagtgat tatatcgata cattattggg tgaagtatgg gtagtggctt
    16861 tgatgctctt gctgggtgga atattaatga tatttatcga tcaatggctg aaatatcctt
    16921 catccaatca ggaaataagc tggaaacgtg cgctggaaat cggattcttt caatgtattg
    16981 ctatgattcc aggtgtttcc cgttctatgg caacaattgt aggcggaatg gcaacaaaac
    17041 tcaatcgcaa aaatgcagca gaattttctt ttttccttgc tgttcctact atggctgctg
    17101 cttccatttt caaactaatc gaattattac acaccagcaa tggaagtcaa gtactgacac
    17161 aaaatttatc tcttttactt gttgggaata tagtagcttt tgtagtagca atggcagcta
    17221 ttaaattttt tattgatttt ctaacaaaac atggttttaa agcgtttgga tattatcgta
    17281 tcatagtagg tgctttcctc ttaatattaa tttatttggg aatcgattta aaaatagttt
    17341 aatggacgtt attgcaggag aagttttcta tgtagataag ccactgcatt ggacatcatt
    17401 caatgtggtg gaccgaatac gaaagaaaat aacaaaaaaa ctaaaaatta aaaatataaa
    17461 agtagggcat gcaggtacac ttgatccatt agcaacagga gtgatgataa tttgtacggg
    17521 caaggctacc aaatcaattg atttatatca aaatcagaca aaagaatata ttgctacttt
    17581 aaagttaggt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattgattta
    17701 tatcaaaatc agacaaaaga atatattgct actttaaagt taggtgctac aactccttct
    17761 ttcgatttgg aattaccaat tgataaaatt tatccaaccc atcatattac tcttgaaatg
    17821 atcgagaatg ttcttcacaa ttttttggga gaaattgacc agataccacc tatttattct
    17881 gccgttaagg tagatggcga aagagcttac aaatatgcaa gaaaaggcga aaaagttgaa
    17941 ctaaaaccaa aaaaattggt tataaataag ttggaagtat tggaatttaa tttacctctt
    18001 cttaaaattc ggatagaatg cagtaaaggt acttacatta gagctttggc gcgtgatatt
    18061 ggaaaaacct taggaagtgg tgctcacctg gttgcgttgc agcgtacacg cataggagaa
    18121 gtgcgattgg aagattgttg gcaattaaat gacttattac aatatcttga cgatgagctt
    18181 ttgaattctg tccctttcac aaaataaatg gaaaatattc tgtaagattt tttaaaatag
    18241 ctatttaaaa atataataag agcaaaaaca ataatttaaa atataccacg ttgattttaa
    18301 aaattcatct ttttttaatt aaaattagca ataatattct ttataattag ctgttttgat
    18361 tgattttttt tcttttctct ttttaaaaaa taaaaaggta ttaataagaa agtgtgttta
    18421 gtttaacaat atcaatgttt tagttttatg aaattatctc aatttaaatt taagttaccc
    18481 gacgagttga ttgcccaata tccagctccg catcgtgatg aatcacgatt aatggtagtg
    18541 catcgaaaaa caggcgaaat tgagcataaa atctttaaag acgtgcttga ttattttaat
    18601 gaaaatgatc tttttgtatt gaacgataca aaagtgttcc cagctcgatt gtatggaaat
    18661 aaggaaaaaa caggagctca aattgaagta tttttattaa gggaattaaa ccctgattta
    18721 aagctgtggg acgtccttgt agagcctgct cgtaagatac gaattggtaa caagctatat
    18781 tttggcaacg atgattcact tgtagctgaa gtagttgaca atactacttc cagaggaaga
    18841 actcttcgtt tcttatttga tggcactcac gaagaattta aaaaaacact ttattcatta
    18901 ggagaaacac cactgccacg ctacattaat cgctccgttg aaaaagaaga tgccgaacgt
    18961 tttcaaacta tttttgcaaa aaatgaaggt gctgtagctg ttccttcagc aggtttgcat
    19021 tttagccgag aattaatgaa acgaatggaa attaaaggca ttgaaatagc ttttttaact
    19081 tcctatatga atttaggaaa ttttcgtgaa atagatgtag aggatcttac caagcataaa
    19141 atggattcag aacaaatttc tgttccggaa gaaacggctg aaaaagtaaa taaagctcag
    19201 gaaaacagac aaaatatttg tgccgttggt gctaccgtta tgcgggcatt agaatccgta
    19261 gcatgcactg atggcaaaat aaaatctttt gaaggatgga caaataaatt tattttccct
    19321 ccttacgatt ttactgtggc taatgctttg ataaccaatt ttcattttcc ctactcaact
    19381 ttcttgatgt taaccgccgc ttttggtgga tatgatttaa ttatggatac ataccaaata
    19441 gctattgagg aaaaatatcg ttttggagcg tatggagatg caatgctggt aatataaaaa
    19501 tttgaaaatc aacttactgt caaattttac gtaaataagg taactgaaat tgatttttat
    19561 tattcttagt tatctattcg ggtgaaaata actttactat ttttaagaag aaaaggttta
    19621 tttttaaaaa tcttcttaaa aggaaagaga attgcgaata cgaaaatatt ttttaatttt
    19681 gcacgccgct tattaaagga tgaattcaaa ttttcaggat tgtctcatgg tgtaatggta
    19741 gcacgtcaga ttctggatct gcctgtctag gttcg
//
LOCUS       DAOSAS010000015        19576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_1958_length_19576_cov_9.499667, whole genome shotgun sequence.
ACCESSION   DAOSAS010000015 DAOSAS010000000
VERSION     DAOSAS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19576
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1958_length_19576_cov_9.499667"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            608..3826
                     /locus_tag="PLR72_01385"
     CDS             608..3826
                     /locus_tag="PLR72_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK35897.1"
                     /translation="MSLITNYLRVISLVLLLSIFGLSNAQTSNQKVTIELKNVTLKDF
                     IRQIESQTNYTIVYQDTLIDNKKDITIKVVEEPLNKVINTVLDKEGLQATFKNNTIVI
                     TKKNTDSSREKKSVLVSGVVYDEEGMPVIGASITIPGTNRGVITDLDGRFSIEAPLNS
                     TLKITYVGYQPKEIIVTDNPNVKIILQQAVKELEEIVITAQAIGQKNAILKQINSNTL
                     KNVISAEKLQQNPDINTIEAIGRLPGISVIRSGGEGAAFLIRGLDQTYSKVLINGEPL
                     AVGLNAVSPYSLEGVEVYKTLTANQEGDAVAGSVDLTMREAPKGLHYSIMAQSGYNAL
                     NKDFGNYSFVGMVSNRFFNEKLGFFLSLTADRVNRSTNLLNVGYNTNYTTNLNDPFYI
                     NGMNFTLNERINYKQSAVLTLDYIVNKSTTLNFQSFLSTINYYSSSQSKSFNPEGGIA
                     TIPIYVNMSKIPKNRTSGITNNFSGRTTLKFLRSILYYGVSYVYNETDNPGTRTWAYS
                     SKTRADGLSRDSLKVFSPEQVASHFDKILGNLKETELESMSYDQTYAKNWSLTPRIDF
                     EIPIKSNSDLVHGKIQLGAKYRLSHNYFDRNVGIAAVGNNSVFIDFIKNNYNYYWPSG
                     IVELTVAGQENNFLGGSYNFGDKYSFERNDLVFDAWLQHGKETYLAGPTGGLSDDPRY
                     SGFVYDLYSSAMNDLDDINQYGAGYIMPEINIGKWLMIMPGVRFEYGRTKMKAYSGNE
                     VTRPYSVFQDINSAYSLKDSIAIREDKFFLPMLHLRIKPIDWFYTHFSYTHTLRRPDL
                     SMISPFEYYNAQDASSYSYSAGNPNLKTELWKSYDLQLTFHGRKAGLFSITGFKKTVK
                     DKLWSRTYKRIQGEPIPNPVFKDNDLVNMTVYENHPYSILLEGIETEWQTSFGYLPKP
                     FSYLTLSANYTYTHGKSPNPYTRLYKYKPEGSRYELTGRKDSVVIEPMTGVPEHVFNL
                     TMGIEVKNFKSYLSYQYSSEKILQTHPNDLRLYVISEPYSRLDFNASYGFDFKNKDIL
                     EIILRTANLTNSEDRVRYRGEHRPISVEQYGITVDLGLRYKF"
     gene            3876..5666
                     /locus_tag="PLR72_01390"
     CDS             3876..5666
                     /locus_tag="PLR72_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK35898.1"
                     /translation="MKKLNLLLILCTLTCAFTTSAQDTLDVVPGIGTLNAAIKQYKGG
                     KIYRLKDGYNGYYVLNEIIDNTGFDLTIVGGGKPDANDPKPTMPATLQTSGTGGTPFN
                     FMFNVLSNITLKGIYFINATADGVFNTQFFMGIQGNNIRVIIDKCVLDPSGSPIYCPG
                     GSPKIYLTNNLFNRLTNQTTSVNGPVNFYFPNDQSGVDTLYIENNTFIGLSTAVFSDN
                     FAPVKSGFTWINHNTFVHHKCQIDWMANQEKFYFTNNLLYDCHVIPYERAWVGGWDNY
                     PTGAVSELLWSYPDSKVKDNGVDIDWGFDKMTSFVANNIEFKNQKFFDNLEDLYSWTA
                     SVGAPNSFYFQPLVWTPDVPCENRGIDINTALANNPQASIYNSEQYPTWKAANNRYDI
                     DPSFTDTRIKQKSDILAQWALPAIKKDYFGSYYTNTDHTSLDWYWDPDGNMGQNETWP
                     LFDGIYSNTEALNFGLDGLPAGDLNWYPAKKEIWEKNKAAIESHILSLNTTKMDLSSV
                     RPIYGEKVVHIDIYPNPATDIIKINFNKPIKTIIEISVIDVLGRTVDMINSNVSEESI
                     FYNSSALKPGIYYLKAKVDGIDQQGVFIKK"
     gene            5692..8310
                     /locus_tag="PLR72_01395"
     CDS             5692..8310
                     /locus_tag="PLR72_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK35899.1"
                     /translation="MKQLFALLFSFFVWVVLSAQTMNDVPSGLRLVAPAHYSYGCAVA
                     NDSSVYFTEFNNQQLRQVLPNGIVQVRRMGLSGMFGLSFDPSGNLFIGRDLGDVGNPG
                     KITRITPTGVETDIVTGITRPRGLATDTSGNVYFATESPSRISRWNKNTGTIDVLANC
                     SYAAEGVTVASDGSIYFSEYGDPSGGICGSVKKRDINGTITTILDTGIWRCRGLVINS
                     SNDYLYLCTEADQADHGNSGFLAKIKIADGTWSKALEGIDYPQYPAIGPDENIYFTMT
                     RENWLAKYDPTASTNISDWSGNSNIKIGISEGQWTSTGITNTLTIKVGKTLSFTGHIS
                     PSVSNGTVYGWIRIPEDIITLDSTVLYSPCYTSEHPCPGIYKLPYITYETDTGSCFIS
                     ALALRNHIGQRWPMQNPGTCNETPAAGFDEKPEAYLIYFSWNKTDRMAAILSPSYNEN
                     GNKMTIIKGSGSGWIYSGCVWSPAGQTWLNSGYYQSNPDATSWAELDLGSFSGKSKYI
                     YSMWHKNGSYRPNSAVFRLYDYTEEKFNTIVDETKHADNLNHADDTFSGWYLIGNKRI
                     NITPSTKLRIYQNAPVVSNEYLQSDAILLSDYPIVDNTSLGSASDFESNPILSVESTG
                     ATGLGNHWSMQGIGYQFSTTNGKSFGNKLDTNIFTDLKDEDYYVEVSWDYLNNDNINV
                     TNARYTVNGSQTSDIINQNRSSSNQSGSFIQGNSVGTWSGFYRLNGTYAHSETNNLLV
                     TSNYSTSLYNSKRFVYDMIRFIPIDNEFKRYNSPANSYDNDLFASEENKTKRVIVYPN
                     PFVDIVKFNINLKSYDNTNTEIRIINILGQTIEILNCRNGVPELEWDGSKLKPGIYLY
                     NVKINGINESGVLFKK"
     gene            8403..9173
                     /locus_tag="PLR72_01400"
     CDS             8403..9173
                     /locus_tag="PLR72_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK35900.1"
                     /translation="MKIKLLFLFLWTFSITNAQITWDFKTGTEGWGSYGDDVDVTWDG
                     LDNLVLNYTGGSDGNITYPTIYNDTPLNVANVNYFYIKFMAINWPKSSVLVNIQFEIN
                     GNNYYANQTMNATSGEFSFNIRNEVQHTWSVLPATGTTSRLRIEIPHNSELAGYDWKG
                     ASLKIDKIAFLNSNPTGLIKNEKDELNVFPNPASDVIRIVGQNYCKATIIDMCGKIIK
                     EIDKSTSSISVSNLTKGVYFIKIEKNDNTSVVKKLIIK"
     gene            9227..13390
                     /locus_tag="PLR72_01405"
     CDS             9227..13390
                     /locus_tag="PLR72_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK35901.1"
                     /translation="MKSSGKIFFLAEIKQTITCIIILFLTFSTEVKSQTSWNFDSDLE
                     GWHDLGDGRDVRASWENGYLKMTYCDRTPNDQGSQLWFPAVQVDNLNFSASDYPYLEI
                     YYEAHWPTILVRKILITLTKTDNTVVYSFINIDPAENFISIDIAANEMTSWTGATHYD
                     GQIKSIQLELPHNSSANPATDWFGFYTLIDKIELSATQTPKPDNKKQLKWSFENGFTD
                     DNNQYPCSTIGNPTISTTDFKNGKSALYLDGDSYLIIPANPILNSREQTISFWIKTIG
                     KGQKYSSIATLFSYDEGNYGLALNDEKLVFKKAARVWDNLTLWNGGVWQHVAIVNERN
                     IMTCYINGRKVADLMNVPMTNRSGNIVIGNGGLIAYIDDFSIFNYALASTEIATQGSI
                     PLNTILPWTFQKDLEGWHEIQDGIERDIMLGWEPGIMKLIYTNKALDTGSHLWFPQVE
                     VDTKFDAFLYPYCDIYYQTKDWPVTTPVKALLELTKADSTIVYSFFDVDPTKNFVRVD
                     FSSTIQNSGSSYAGVICKVRLEFPQGALATPAEEWFGASTEIIKIEFTDIKPSVNETW
                     DELLDKSAFIKSGMEKLNRVDYQYQPIGLGGTTMRVDPWGFAYQKAPMASSGTSWRLH
                     KPYLGYEYWWDNAGHRFNPFLLKAGYGATLDPGTITAFNQKLDIRTGELSIDLGLMVD
                     GTSFTSKRNIFVTPDGILVIRVQDNGAPSPLQVNLAIDSIVRIYNNFGIYNVPHDPWE
                     GSATAREQNTTKGIVVTATRPNTSIASLAVAIESSSSVTVSADNQIVSTSEPNGIVTY
                     YVAPTSSFNPLTPTVPWDHAWNAAYAAKEKGYEALRQETVAWWSNYFSRSKISVPDEN
                     VLKLYVQSLFYEGVYFGNTKIPPGCNGTDIESFAGAICPEYDLVFSQLALLYSGHIAE
                     AKNIAEWTYNVLPRAKANAVNGITHHNVTRKYDSGAIYTTLMGYDGTLCVQPTEGEGI
                     NLYENYPGANASLMALSYLDSSNDNTFTEKALDILKSTTYVSLQDLIYNGKTYQCKNM
                     PNTVQQAADLYGYNQCVKRGIADPGWAVYDGKILIPQTTLFGDTLIAGGVGAPAEEGV
                     GDATWLFPLWWYGVVDKQDKKVLPSYINVAKSNTGDYVFNNGTMGIIAAKLGLGNDAL
                     SWLQRFQSDNILYDETCFTESKGQLYLTPEIGAHGSYICNLTQMLLDPDNEHIIDVFP
                     AIPDSWEYRKIGFDSLMVTGGIRITATRDIEGVRLELKNTSLNEINRIVRIAIPRMMD
                     VDNLNTSSMISGKIVNNVSLQPGETKILEYSYKPSKIIASVNNININDDAIKYKIFPN
                     PNSTGQLRIMNGQDVKLIRIYNLKGELVKILIGNSETYFIGKLPQGIYILNMELKTSK
                     HVNHLLLIK"
     gene            13439..15145
                     /locus_tag="PLR72_01410"
     CDS             13439..15145
                     /locus_tag="PLR72_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35902.1"
                     /translation="MRKIILFYVTILLLLSQLIFAQKKTDPSKNGRGGFDYFVGIVGS
                     PSVINDIKWDDQCLKSLKELGVNVLQLSVAWGGKPANEVLNMEDLDEEQIAKWKFRIA
                     QCKKFGFKPLAHFGVPRVLNSDPVKPACISDPDVQQKYVQMMKKFLNTFPEVNDILIY
                     TYDQQAWICSEFGPCSRCSGIPISDRLPNFLNLLKKTMQDCRPQTQTTLWWKPWELSK
                     GETIDILKKIDPHNFGLVLNSSSSNEVYPFNDRSFKSDLGVKRLVQYAFEHNIPAIGE
                     FDHTLYKGLYQIEDYFPRLIYEQMEGWKELKGVVGVKEYYGFAPSTYSVNYAMLHAWM
                     KNPNASLDELLNQIAAPYGKKTAPLMIKAWEYVAQSVEAFPWDVTYMIGPMGLDKSEN
                     GEHDWNCIKIINATWDTPIWESNRRANFMLTDSKVAHPWIFEDASLRLEDAAQLSFEA
                     VEYYNQAIAQNENNVADIIKQRDFVQHTGRSLRGKGIHFALTIASQDARTVAFDKGQF
                     EKVCKRIEQLLELDVNNGYKMAAIKLEEFKKDPKSWLKNNFYPLTWKSEAEPNWSKWI
                     AP"
     gene            15604..17019
                     /locus_tag="PLR72_01415"
     CDS             15604..17019
                     /locus_tag="PLR72_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HOK35903.1"
                     /translation="MKTRVGLFGIGLDTYWMQFDGLLEKLKIYQSEISKKIKNFGVEV
                     VDAGMVDNPLKASEAANYLKRNDVEIVFLYVSTYALSSTVLPVAQKLKVPIIILNLQP
                     VAALDYNMFNKLGDRGKMTGVWLEHCQSCSVPEIAGVFNRTNISYDIVSGYLQDEKAW
                     KEIKEWTEAAKVAKALRHHRMGILGHYYGGMLDVYTDLTRQSSFFGTHFEMLEMCELK
                     KYRDEVTPEELNTKIAEFHTAFDVSSECDPAEIERAAYTSIALDKLVTVHGLDSLAYY
                     YEGQVGNEYENMVTSVIAGNTLLTGKNIPVAGECEVKNAQAMKIMAEFGAGGSFSEFY
                     LIDFKDDVIYLGHDGPAHFAIAEGQVKLVPLPVYHGKPGKGLSIQMTVKHGPVTLLSV
                     VEGKEKIFLLVAEGKSVTGPTLEIGNTNSRYRFSLSAKDFIDRWSKQGPAHHCAIGIG
                     HIADKIEKLGKILNIDVVKIC"
     gene            17050..18849
                     /locus_tag="PLR72_01420"
     CDS             17050..18849
                     /locus_tag="PLR72_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35904.1"
                     /translation="MLKKIISVTFIAILLMACKHKSSDSFQYKVGILGAPSSPFVEWN
                     DHNLELMKKLGFNTMQLNIAWGYRPADNALNLEDVLVVPNEFRLPVDNDSTLNKNNGG
                     LNTYLHSSARIAKRAAELKHRIAICKKHEMRTLFHFGAPYVAYPPIEPLSQCISDEKT
                     IRRYEKLIELFAAQFKGVDDLLLYTYDQNAWLCSEYGPCEKCFGKPLNERVPVFVNAI
                     ARKWHQLNPNGRLWWEPWEISAGQTYKIINKLDSTCIGLAVHSNIAEVQIAFPADRWY
                     KNVVNIANRRSIPVLVEVWLGTATEEIEPYIYLPSPLATLRALKALNNVGKIAGIKEY
                     YGNIPDREDPNLRMSSIFFKNPTISEEEALKKLSQPYGYSKKVQQYWRLSSEAIEMYP
                     WDISWFAREVGRSDPHHSLTEATLKGASWETPSWQSNRRAAFMRTVETDYPHFWMLED
                     AQLRFDCTAQKAEDALNAGIAARNDIPKRMMKEFDSSLEELRGFKQRCLAYTFHIRES
                     NICKMLRSSIKSGKKPNPVLVVELKELLKKDLKNQGENNCLQQAINLLDKNIYQFLQV
                     YFVKPPKKTNTLDMYFNDSIPANVKTVWTITSK"
     gene            18858..19171
                     /gene="rhaM"
                     /locus_tag="PLR72_01425"
                     /pseudo
     CDS             18858..19171
                     /gene="rhaM"
                     /locus_tag="PLR72_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
BASE COUNT         6709 a         3008 c         3669 g         6190 t
ORIGIN      
        1 atatactgta atttatccct tttaaagtag gatactttgc ggataatcat tttttctttt
       61 ttggtgaatt tattcaaaat attgccacaa agcaaaaaaa taaaaaatac caaatatttt
      121 tacaattaac ttgaaaatat gccgactttt cttttgggga cagaagttac gacacattgc
      181 tattgcaaac ctgctaagca gtaatattgt aatcttattt cacttatact gaataaactt
      241 catttggaaa gtcatataca tataccgcct actggttaat aatattgttg ctttacaaaa
      301 agctaatttt aagcaaaaac ttttagattg caaaaaatcg tagaatatca aaacaaaaaa
      361 aatcagtaat tttttcaaaa aatctctttt tccttccttt tgaaaaaatg aagttttaag
      421 cgggaactca ttaatagaaa attagaaaat ttacatttta ggtcaaattt caaagtattt
      481 atgtcaaata ccttactttt tttggttact atttctttta tctttgttaa aactattatt
      541 tggctaaata atagatcttt attcattcga aaataatgtt gttttataat tcataaaata
      601 atagacaatg tccttaataa ctaattattt aagagtaatt tcactcgttt tgctcctatc
      661 tatctttggc ttatcaaatg cgcagacttc gaatcaaaaa gtaacgatag aacttaaaaa
      721 tgttacgttg aaagatttta ttagacaaat tgaatctcaa acgaattata ctatagtata
      781 tcaagataca ttgattgata ataaaaaaga cattacaatt aaagtggttg aggaaccatt
      841 gaacaaggtg attaatactg tacttgataa agaaggatta caggcgacat tcaaaaacaa
      901 tacaattgta attacaaaaa aaaatactga ttcctcacgt gaaaaaaaaa gtgttttggt
      961 ctcaggggtt gtatacgatg aggaaggcat gcctgttatt ggtgcaagta ttactatacc
     1021 aggaactaat aggggagtta taacagattt ggatggtcgg tttagtattg aagcaccgct
     1081 taattcgaca ttaaaaatta cctatgtagg ttatcaacca aaagaaataa tagtgacgga
     1141 taatccgaat gtgaaaataa ttttgcagca agcagtaaaa gaattggagg aaattgttat
     1201 aactgctcag gcaataggcc agaaaaatgc tattctcaaa caaatcaatt caaatacatt
     1261 gaagaatgtt atatcagctg aaaaacttca acagaatcca gatataaaca ctatcgaggc
     1321 aatcggacgt ttacctggaa tctcggtaat tcgatctggt ggagaaggag ctgcattttt
     1381 aatacgtggt ttagatcaga cttattccaa ggtattaatt aatggagaac cattagcagt
     1441 tggtttaaat gcagtttcac cctattcact agagggagtt gaggtatata aaaccttgac
     1501 agctaatcaa gagggagatg ctgtagcagg gtcggtagac ctgacgatga gagaagctcc
     1561 caagggttta cattattcta taatggcaca atcagggtat aacgcattaa ataaagattt
     1621 tggaaattat agttttgtag gcatggtaag taatcgcttt tttaatgaaa agttgggatt
     1681 ttttctctct cttactgctg acagagtaaa tagaagtact aatctgttga atgtaggtta
     1741 taatacaaat tatactacta acctaaatga tcctttttat atcaatggga tgaacttcac
     1801 tcttaatgaa cgaataaatt acaagcaatc tgctgttctt acattagatt atattgttaa
     1861 taaatctacc acgcttaatt tccaatcttt tttatcaact attaattatt actcttcaag
     1921 tcaatcgaaa agttttaatc ccgaaggtgg cattgccact atacctatat atgtaaatat
     1981 gagtaaaatt ccaaaaaata ggacctcggg aattaccaat aattttagtg gacgtacaac
     2041 attaaaattt ttaagatcaa ttttgtatta tggtgttagt tacgtttaca atgaaacaga
     2101 taatcctgga actagaacat gggcatatag ttctaaaact agggcagatg gcttatctcg
     2161 tgatagtcta aaagtttttt cacctgaaca ggttgccagt cactttgata agattttagg
     2221 aaacttgaag gaaaccgaat tggaaagtat gagttatgat cagacttatg ccaagaattg
     2281 gagtttaact ccccgcattg actttgaaat accaattaaa agtaattctg acttagttca
     2341 tggtaaaatt cagttgggag ctaaatatcg actttcgcat aactactttg accgcaatgt
     2401 aggtatagct gctgtaggga ataatagtgt ttttattgat tttattaaaa ataactataa
     2461 ttattattgg ccttcaggaa ttgtagaatt gacagttgcg gggcaggaaa ataatttttt
     2521 aggtggaagt tataatttcg gggataaata ttcttttgaa cgaaatgatt tagtttttga
     2581 tgcgtggctg caacacggta aagaaaccta ccttgccggt cccacaggag gcctcagtga
     2641 tgacccaaga tattcaggat ttgtatatga tttgtattca tcggctatga atgatttgga
     2701 cgacattaat caatatggag ctggctatat tatgccagaa attaacattg gtaaatggtt
     2761 gatgattatg cccggagtga gatttgaata tggtagaaca aaaatgaaag cttacagtgg
     2821 aaatgaagtt acacgtccat actccgtttt ccaagatata aattcggctt acagcttaaa
     2881 agattctatt gctattcgtg aagataaatt ttttcttcca atgttacatt tacgtataaa
     2941 accaattgat tggttctata ctcatttttc atatacacat actctccgac gacccgatct
     3001 cagcatgatt tctccttttg aatattataa tgctcaggat gcatcaagct attcttacag
     3061 tgcaggtaat cccaatttaa aaactgagtt atggaagagt tatgatttac agttaacttt
     3121 tcatgggagg aaagcaggct tgttttctat aacaggattt aagaaaactg ttaaagataa
     3181 gttatggtca agaacttaca aacgtattca aggagagcct atcccaaatc cagtttttaa
     3241 agataatgat ctggtgaata tgactgtata tgagaatcac ccttattcca ttttattaga
     3301 agggattgaa acagaatggc aaacttcatt cggatattta ccaaaaccat tttcgtattt
     3361 gacgttaagt gctaattata cctatacgca cggaaaatct ccaaatccat atactcgttt
     3421 atataaatac aaacctgaag gatctcggta tgagctaact ggaagaaaag actcggtggt
     3481 gattgaacca atgacaggag taccagaaca tgtgtttaat ttaacaatgg gtattgaggt
     3541 caaaaacttt aagagttatt tatcttatca atattcatca gagaaaatac tacaaactca
     3601 tccaaatgat ttaaggctgt atgttatttc ggagccttat agtagattgg attttaacgc
     3661 ttcctatggt tttgatttta aaaacaaaga catattggaa ataatactta gaacagctaa
     3721 tttgactaac agtgaagacc gtgttcgata tcgtggtgaa catcgtccta ttagcgttga
     3781 acaatacggt attactgttg atttaggact aaggtataaa ttttaaaaaa atattatcaa
     3841 aatagtttta ttattcactt ttaaatcaca atgtcgtgaa aaaattaaat ttacttctga
     3901 ttttatgcac gttaacgtgt gcatttacta catcagcgca agacactctt gatgtagttc
     3961 ccggaattgg tactctcaat gctgctatca agcagtataa aggagggaaa atctatcgtt
     4021 tgaaagatgg ttataacggt tattatgttt tgaacgaaat tatcgacaac actggctttg
     4081 atcttacaat tgtaggaggg ggtaagcctg acgcaaatga tcccaaacca acaatgcctg
     4141 ctacacttca aacaagtgga acgggtggta ctccttttaa ttttatgttt aatgtgcttt
     4201 ctaatattac tctcaagggt atctatttca taaatgctac ggcagatggc gtttttaata
     4261 ctcagttttt tatgggaata caaggtaaca atataagggt tattattgat aaatgtgtgt
     4321 tagatccttc cggtagtcca atttattgtc ctggaggaag ccctaaaatt tatcttacaa
     4381 ataatttatt taatcgactc actaatcaaa ctacaagtgt aaatggacct gtgaattttt
     4441 attttcctaa tgatcaaagt ggagtggaca ccctatatat tgagaataat acatttatag
     4501 gtttaagtac cgctgtattc tcagataatt ttgctccagt taaaagtggt ttcacctgga
     4561 taaatcataa tacctttgtt catcataaat gtcaaataga ctggatggca aatcaagaaa
     4621 aattctattt taccaacaat ttgttatatg attgtcatgt aattccatat gaaagagcat
     4681 gggttggagg ttgggataat tatcctacag gagcagtttc agagcttttg tggtcatatc
     4741 cagattctaa ggtaaaggat aatggagttg atattgattg gggatttgac aaaatgacca
     4801 gttttgtagc caataatatc gaatttaaaa atcaaaagtt ttttgataat ctagaagatc
     4861 tatactcatg gacggcaagt gtaggggctc ctaattcgtt ttatttccaa ccgttagtat
     4921 ggacacctga tgtaccttgt gaaaataggg gcatagatat taatacagcc cttgcaaata
     4981 atccgcaagc atctatttat aatagtgagc aatatcctac ctggaaagct gcaaataaca
     5041 gatatgatat tgatccttcg tttacggata cgcgtattaa acaaaaatct gatattcttg
     5101 ctcagtgggc gttaccagca attaagaaag attattttgg ttcttattat accaatactg
     5161 atcatacttc tcttgactgg tattgggacc cagatggaaa tatgggtcaa aatgaaacat
     5221 ggccactctt tgatggaata tattctaata ctgaagcttt aaatttcgga cttgacggat
     5281 tgcctgctgg agatttgaac tggtatcctg ctaaaaaaga aatatgggag aaaaacaaag
     5341 cagctattga atcacatatt ttatcactta acaccacaaa aatggatcta tcctctgttc
     5401 gtccgattta tggtgaaaaa gtcgttcata ttgatatata ccccaatcct gcaacagata
     5461 ttataaaaat caactttaat aaaccaataa aaactattat tgaaattagt gttattgacg
     5521 tattgggtag aacagttgat atgatcaata gcaatgtctc tgaagaatca attttttata
     5581 attcttctgc attaaaacca ggaatttatt atctgaaagc caaggttgac ggaattgatc
     5641 aacaaggcgt atttataaaa aaatagatca atatactaaa gatttataac catgaaacaa
     5701 ttatttgctc tcctattttc attttttgta tgggtagttt tatctgctca aacgatgaat
     5761 gatgttccaa gtggacttcg cttagttgct ccagctcatt actcatatgg atgcgctgta
     5821 gcaaatgata gttcagttta ttttactgaa tttaataatc agcaattaag gcaagtgctt
     5881 ccaaatggca tagttcaggt aagacggatg ggattgagtg ggatgtttgg tctctccttt
     5941 gatccctctg gcaatctttt tattggccgt gatttaggtg atgtaggtaa tccaggtaaa
     6001 ataactagaa taactccaac aggcgttgaa accgatattg ttactggaat tacaaggcct
     6061 agaggacttg caacggatac ttctggtaat gtatattttg caactgaatc accttctcgt
     6121 atttcaaggt ggaataagaa tactggaact atcgatgttt tggccaactg ttcatatgct
     6181 gctgaaggcg tgactgttgc gtctgatggt agtatttatt ttagtgaata cggggatcca
     6241 agtggaggta tctgtggaag tgttaaaaaa agagacataa atggaactat tacaactatt
     6301 ttagatacag gaatatggcg ttgtagagga ttagttataa atagttccaa tgattacttg
     6361 tatttatgta cagaggctga tcaggctgat catgggaatt ctggttttct ggcaaaaata
     6421 aaaattgctg atggtacttg gtctaaggcg ttagaaggaa ttgattatcc ccaatatccg
     6481 gctataggac ctgatgaaaa tatttatttt actatgacaa gagaaaattg gcttgcaaaa
     6541 tacgatccta ctgcgagtac caatataagt gattggagtg gtaactcaaa tattaaaatc
     6601 gggataagtg aaggacaatg gacatcgact ggaataacaa atacgttaac tattaaagta
     6661 ggtaaaactc tttcttttac cggacatatt tctccgagtg tctcaaatgg tacggtgtat
     6721 ggatggatta gaataccgga agatataata acactagatt ctacagtgtt gtactcgcct
     6781 tgttatactt cagaacatcc ctgtccggga atctataaac tcccctatat tacctatgaa
     6841 acagatacag gttcctgctt tatttctgca cttgctcttc gaaaccatat tggtcaacgg
     6901 tggccaatgc aaaatcctgg aacatgtaat gaaactcctg ctgcaggttt tgatgaaaag
     6961 ccagaagctt atcttattta cttttcatgg aataaaacag acagaatggc agcaatccta
     7021 tcaccttcat ataatgaaaa tggtaataaa atgactatta taaaaggaag tggttcaggt
     7081 tggatataca gtggttgtgt ctggagtcct gctggtcaga cttggctcaa ttcaggttat
     7141 tatcaatcta atcctgatgc aacttcgtgg gctgaattag atttaggatc attctcaggt
     7201 aaatcaaaat atatttattc aatgtggcat aaaaatggat cttatcgtcc aaattcggct
     7261 gtatttagac tctacgatta taccgaagaa aagttcaata ctattgttga tgaaacaaaa
     7321 catgcagata atttaaatca tgccgatgac actttttcgg ggtggtactt gattggtaat
     7381 aaaagaatta atataacgcc aagtactaaa cttcgtattt atcaaaatgc tccagtagtt
     7441 tcaaacgaat atttacaaag cgatgcaata cttttatcag attatccaat tgtagataat
     7501 acatctttag gatcagcatc tgactttgaa tcaaatccga ttttgtcggt tgaaagtaca
     7561 ggagcaactg gattaggtaa ccattggagt atgcaaggaa ttggttatca gttttcaacc
     7621 acaaatggta aatcatttgg aaataaactt gacactaata tttttacaga tttaaaagat
     7681 gaagactatt atgtggaagt ttcctgggat tatttaaata atgacaatat aaatgtgact
     7741 aatgcaagat ataccgtaaa tggaagtcaa acgagtgata taataaatca gaatcgctca
     7801 tcttctaatc aaagtggttc gttcatacaa gggaattcag taggaacatg gtctggtttt
     7861 tatagattga acggtaccta tgcacatagt gaaactaata atcttctcgt aacatcaaat
     7921 tatagtacct ctttatataa ttcaaaaaga tttgtatacg atatgattag atttatacca
     7981 atagacaatg agtttaaaag atataattct ccagccaatt cttatgataa tgatctattt
     8041 gcatcagaag aaaataaaac taaacgtgtt attgtatatc ccaacccttt tgttgatatt
     8101 gttaaattca atattaattt aaagagttat gataatacaa atacggaaat tcgtataatc
     8161 aatattctgg gtcaaactat tgaaattctc aattgcagaa atggtgttcc tgagttggaa
     8221 tgggatggtt caaaacttaa accaggtata tatctataca atgtaaaaat caatggtata
     8281 aatgaaagtg gggtgttatt caaaaaataa gataaatcat tttatttcat tttatctttt
     8341 tctgatacaa gtaaaaaatt aattaataaa catattgttg aatacttaaa aaatttataa
     8401 ccatgaaaat caaactttta tttctattct tatggacatt ttctataact aatgcacaaa
     8461 taacatggga ttttaaaact ggtacagaag gatggggatc ttatggagat gatgttgacg
     8521 tcacatggga tggcttagat aatcttgtcc ttaattacac tggaggtagt gatggaaata
     8581 taacttatcc tacaatttat aatgatacac ctcttaacgt cgcaaatgta aattatttct
     8641 atataaaatt tatggcaata aattggccaa agtcttctgt ccttgtaaat attcaatttg
     8701 aaataaatgg aaataattat tatgcaaatc aaaccatgaa tgccacttct ggcgaatttt
     8761 cgtttaatat ccggaatgaa gtacaacaca catggagcgt tttgccagcg acaggaacta
     8821 cttcaagatt aaggattgaa attccccata attctgaatt agcaggttat gactggaaag
     8881 gagcaagttt gaaaattgat aaaattgctt ttttaaattc aaaccctact gggttaataa
     8941 agaatgaaaa ggatgaatta aatgtttttc ctaaccctgc ttctgatgtt attcgtattg
     9001 tagggcaaaa ctattgcaaa gcaacgataa ttgatatgtg cgggaaaatt ataaaagaaa
     9061 ttgataaatc tacttcttct atatcggttt caaatctcac aaaaggagtt tattttatta
     9121 aaattgaaaa aaatgacaat acttcggttg taaaaaaact tattattaag taattaaatt
     9181 ctcgggggaa atctttaatt tcccccgaag aaatttttta ttaattatga aatcaagtgg
     9241 taaaatattt tttttagcag aaatcaaaca aacaataact tgtataatta ttttattttt
     9301 aactttttct acggaagtaa aatcccagac ttcatggaat tttgattccg accttgaggg
     9361 ttggcatgat ttaggcgatg gacgcgatgt aagagcaagt tgggaaaacg gttatttaaa
     9421 aatgacttat tgtgaccgta cacctaacga tcagggatct caattatggt ttccggcagt
     9481 acaagtcgac aacttgaatt ttagcgcatc agattatccc tatcttgaga tttattatga
     9541 ggcacattgg ccaacaatat tggttaggaa aatattgatt acactcacta aaacagataa
     9601 tactgttgtt tattctttta tcaatattga tcctgctgag aattttatat ctatagatat
     9661 tgctgcaaat gaaatgacta gttggacagg agcaactcac tatgatggac aaataaaatc
     9721 aatacaatta gaattaccgc ataattcgag tgctaatcca gctactgatt ggtttggctt
     9781 ttatacactt attgacaaaa tagagttaag tgcgactcaa acacccaaac cagacaataa
     9841 aaaacaatta aaatggtcat ttgaaaatgg ttttactgat gataataatc aatacccatg
     9901 ttctaccatt ggtaatccta ccataagtac aactgatttt aaaaatggta aaagtgcact
     9961 gtatttagat ggtgacagtt atctgattat tccggcaaat cctattttaa attctagaga
    10021 gcaaacaata tcattctgga taaaaaccat tggtaaagga caaaagtata gttctattgc
    10081 aactcttttc tcgtatgatg aagggaatta cggtttagct ttaaatgatg aaaaattggt
    10141 tttcaaaaaa gcagcgcgtg tttgggataa tttaacttta tggaatggag gagtatggca
    10201 gcacgtagcg attgtaaacg aaagaaacat aatgacttgt tacattaatg gaaggaaggt
    10261 tgccgactta atgaatgttc ctatgacaaa cagaagtggg aatattgtta ttgggaatgg
    10321 tggactaata gcttatattg atgatttttc aatatttaac tacgcccttg cttcaactga
    10381 aatcgctacg caaggttcaa tcccattaaa taccattcta ccatggacgt tccaaaaaga
    10441 tcttgaggga tggcatgaaa ttcaagatgg aattgaaaga gacataatgc tgggttggga
    10501 gccagggata atgaaattga tatataccaa taaagcattg gacaccggtt cccatctatg
    10561 gttccctcaa gtggaagtag atacaaaatt tgatgcgttt ctttatccct attgtgatat
    10621 ttattatcag acaaaagatt ggcctgttac tacgcctgta aaagcattgc tggaattaac
    10681 aaaagcagac agtacaatag tttattcatt tttcgatgta gaccccacaa aaaattttgt
    10741 aagagttgat ttttcctcga caattcaaaa tagtggttct agttacgctg gtgtaatatg
    10801 taaagtgcga ctggaatttc cacaaggggc tttggctaca ccagccgagg aatggtttgg
    10861 tgcttccaca gaaattataa agattgagtt taccgatata aagccttcag taaatgaaac
    10921 gtgggatgaa cttcttgata aaagtgcttt tatcaaatca ggaatggaga aattaaaccg
    10981 tgtagattat caatatcagc ccattggatt gggaggaact actatgcggg tagatccttg
    11041 gggatttgct tatcagaaag cgccaatggc aagttccggt acttcatggc gtttacataa
    11101 gccatattta ggatatgaat attggtggga caatgcaggg cacaggttta atccattctt
    11161 actcaaggca ggctatgggg ccacactcga tccgggaaca attactgcct tcaatcagaa
    11221 attagacatt cggaccggag aacttagtat tgatctcggt cttatggtag atggaacttc
    11281 gttcacttca aaacggaata tatttgtaac acccgacgga atattggtaa ttagagttca
    11341 ggataatgga gcgccttcgc cattacaggt taacttagca atagattcca ttgtacggat
    11401 ttataataat tttggtattt acaatgtacc acacgatcca tgggaaggtt cggcaactgc
    11461 tcgtgagcaa aacactacaa aaggcattgt tgtgactgct acccgaccta ataccagcat
    11521 agcttcactg gctgttgcga ttgaaagttc ttcgagtgtt actgtgagtg cagacaacca
    11581 aattgtgagt acaagtgaac ccaatggcat tgtaacttac tatgtagcac caacttcttc
    11641 tttcaatcca cttactccta cagttccttg ggatcatgca tggaatgcag cttatgcagc
    11701 gaaagaaaaa ggatatgaag ctcttcgtca ggaaactgta gcttggtgga gtaattattt
    11761 cagtcgttcg aaaatatcag ttcccgatga aaatgtacta aaactttacg ttcaatcgct
    11821 tttttacgag ggcgtgtatt ttggaaatac aaaaattcct cccggttgta atggtactga
    11881 tattgagagt tttgctggtg ctatttgtcc ggaatatgat ttggttttca gtcagttggc
    11941 attactttat tctggtcata ttgctgaagc aaaaaacatt gctgagtgga catataatgt
    12001 attaccaaga gctaaagcaa atgctgtaaa tggaatcact catcataatg taactcgtaa
    12061 atatgacagt ggtgctattt acaccacatt aatgggatat gatggcacac tttgcgttca
    12121 accaactgaa ggtgagggaa ttaatcttta tgaaaattat cctggagcta atgcctcatt
    12181 aatggcgctt tcgtatcttg attccagtaa tgataatact ttcactgaga aagcgttgga
    12241 tattctgaaa tcaactacct acgtttcctt gcaggatttg atatacaacg ggaaaacgta
    12301 tcaatgtaaa aacatgccaa atacagttca gcaagcagca gatctttacg ggtataatca
    12361 atgtgtaaaa cgtggcattg ctgatccagg atgggctgta tacgatggta aaatattgat
    12421 tcctcaaact accttatttg gtgatacgct tattgcagga ggtgttgggg ctccagccga
    12481 ggaaggcgta ggagatgcta catggttatt ccctctttgg tggtatggag tagttgataa
    12541 acaggataag aaagtgttgc caagttatat taatgttgct aaatcaaata ctggtgatta
    12601 tgtatttaat aatgggacaa tgggaattat tgcagcaaaa ttaggattgg gaaatgatgc
    12661 tttgtcatgg ttgcaaagat tccagtctga taatattcta tatgatgaaa cctgtttcac
    12721 agaatcaaaa ggtcaattgt atttgactcc agaaatagga gcccatggta gttatatttg
    12781 taatttgact caaatgttac ttgatcctga taatgaacat attattgatg ttttcccagc
    12841 aatacctgat agctgggaat atcgaaaaat aggattcgat agtttaatgg ttacaggggg
    12901 aatcagaata actgcgacga gggatataga aggagtccga ttagaactaa aaaatacatc
    12961 tttaaatgaa ataaatagga tagtaagaat tgcaattccc cgcatgatgg atgtggataa
    13021 tttaaatacc tcatctatga tttccgggaa aatagtaaac aatgtttctt tacaaccggg
    13081 cgaaacgaaa atacttgaat atagctataa accatcaaaa attattgcct ctgttaataa
    13141 tataaatata aatgatgatg caattaaata taaaatattc ccgaatccta atagtactgg
    13201 gcagcttcga ataatgaatg gccaagatgt aaagttaatc agaatttata atttgaaggg
    13261 agaattagtt aaaatcttaa ttggaaattc agagacttat ttcatcggaa agttaccaca
    13321 agggatatat atattgaata tggaactaaa aacatctaaa catgttaatc atcttctttt
    13381 aattaaataa tataactatt ttattaatcc ggctattgcc atttttatat tttatgaaat
    13441 gagaaaaata attttatttt atgtgactat tttattgttg ttaagtcaat taatttttgc
    13501 acaaaaaaag actgatcctt caaaaaatgg gcgtggaggt tttgactatt ttgtaggtat
    13561 tgttggtagt ccttctgtta taaatgacat aaaatgggac gatcaatgcc ttaaaagtct
    13621 taaagaactt ggggtgaatg tgcttcagtt aagtgtagca tggggaggga aaccagctaa
    13681 tgaagttttg aacatggaag accttgacga agagcaaatt gcaaaatgga aattcagaat
    13741 agctcaatgt aaaaaatttg gatttaaacc attggctcat tttggagtgc cacgagtgct
    13801 taatagtgat cctgtaaaac cagcttgcat atctgatcct gacgttcagc agaaatatgt
    13861 gcaaatgatg aaaaagtttt tgaacacttt tcctgaagtg aatgatatac tgatttacac
    13921 ttatgaccag caagcttgga tttgtagtga gtttggtccc tgttctcgct gtagtggaat
    13981 tcccatcagt gacaggctac ctaatttttt aaatcttttg aagaaaacca tgcaggattg
    14041 tcgtccgcaa actcaaacaa ctttatggtg gaaaccttgg gaactttcaa aaggcgagac
    14101 aatagatatt ttgaagaaaa tagacccaca taattttggt ttagtgctta attcatcatc
    14161 aagcaatgaa gtatatccgt ttaacgatcg ttcatttaag tcggatttag gagtaaaacg
    14221 tctcgttcaa tatgcttttg aacacaatat tccagcgata ggtgagttcg atcataccct
    14281 ttataagggt ttatatcaaa ttgaagatta ttttccgcgt ctcatatatg agcagatgga
    14341 gggttggaaa gagctaaaag gtgtggtagg tgtaaaagaa tattatggtt ttgcaccatc
    14401 tacttattca gtaaattacg caatgcttca tgcatggatg aaaaacccaa atgcttcatt
    14461 ggatgaattg ttgaaccaga ttgctgcacc gtatggtaaa aaaacagctc ctcttatgat
    14521 aaaagcctgg gaatatgttg cacaatctgt ggaagcattt ccatgggatg taacttatat
    14581 gattggacca atgggattag ataaaagtga aaatggagaa catgattgga attgtataaa
    14641 aataattaat gctacatggg atacacctat ttgggaatcg aatcgtcgcg cgaattttat
    14701 gcttaccgat tcgaaagtgg ctcatccatg gatttttgaa gatgctagtt tgagattgga
    14761 agatgcagct caactcagtt ttgaagctgt agaatattat aatcaggcca ttgctcaaaa
    14821 tgaaaacaat gtggcagata ttatcaaaca gcgggatttt gtacaacata ccggacgttc
    14881 gctgcgtggt aaaggaatcc acttcgcact taccattgcc tcacaagatg ccagaaccgt
    14941 agcatttgac aaaggtcaat tcgagaaagt atgtaaacgt attgaacaat tgcttgaatt
    15001 ggatgtaaac aacgggtata aaatggcagc cattaaactt gaagagttta aaaaagatcc
    15061 taaaagttgg cttaaaaaca atttttatcc tttaacgtgg aaatcggaag ccgaaccaaa
    15121 ctggagtaaa tggattgctc cataataaaa ttatgtaaca gaaacccata atcattgatt
    15181 gaatagtgtc gaaaatgctt gattatgctg gacatttgtt ccatactatg aaattgaaaa
    15241 gccacaagat atgttttgag gaagaatata attaaatttt tgaaaaaata aatttaaagt
    15301 tggagctgac tacatattat gaacacaatt tgattgcttg taagtcttga ttttaatatg
    15361 attccccttg ttccgcgaga agtcagaaga ataaatgaat gcattcatta tctaattaaa
    15421 tgtaggatag cattttaaca tagtaacaaa ctttttgaaa taggaatata ctccaaaaaa
    15481 atatgattat tgatttattt ttccataagg ttctcaaaaa aataagaaag tagattaaaa
    15541 caattatata ggaactcgta ttagagaaaa agtttaagct gtattaattt ttaagattta
    15601 agaatgaaaa ctagagtagg attatttggg ataggtttag acacctattg gatgcaattt
    15661 gatgggttgc ttgagaagtt gaaaatatat caaagcgaaa taagtaaaaa aataaaaaat
    15721 ttcggtgttg aagtagtgga tgctgggatg gtcgataatc ctctaaaagc tagtgaagca
    15781 gctaactact tgaaaagaaa tgatgtggaa atcgtttttt tatatgtttc aacatatgca
    15841 ttgtcgtcaa ccgtattacc tgtggcacaa aaactcaaag tgccgataat tatactcaat
    15901 cttcagcctg tcgcagcatt ggattacaat atgttcaata aattgggcga ccgtggaaaa
    15961 atgacaggtg tttggcttga acattgtcag tcttgttcgg ttcccgaaat tgcaggggta
    16021 ttcaatcgca ccaacatctc ctacgacata gttagcggat atcttcaaga tgaaaaagca
    16081 tggaaagaga taaaagaatg gacagaagct gccaaagtag cgaaagccct tcgtcaccat
    16141 cgaatgggga tattgggaca ttattatggt ggtatgcttg acgtgtatac cgatcttact
    16201 agacaaagtt cttttttcgg aacccatttt gaaatgcttg aaatgtgtga gttgaaaaag
    16261 tatagagatg aagttacacc agaagaattg aacaccaaaa ttgctgaatt tcatactgcc
    16321 tttgatgtgt cttccgaatg cgatcctgct gagatcgaac gtgctgctta cacttcgatt
    16381 gctctcgata aactggtgac agttcatgga cttgattcgt tagcttatta ttatgaaggt
    16441 caagttggca atgagtatga aaatatggtc acttcagtca ttgctggaaa tacactgctt
    16501 acggggaaaa acatccctgt agctggtgaa tgtgaagtaa aaaatgccca ggccatgaaa
    16561 atcatggcgg aatttggtgc aggtggatca ttttcagaat tttatttgat tgactttaaa
    16621 gatgatgtaa tatatctcgg acacgatgga cctgcccatt tcgccattgc tgaaggacag
    16681 gtaaagctgg tcccattacc ggtttatcac ggtaaacctg gtaaggggtt atctattcaa
    16741 atgactgtga aacatgggcc agtaaccttg ttgtctgttg tagaaggaaa ggagaagatc
    16801 tttttactgg tagccgaagg gaagtcagta acaggaccta cactggaaat tggaaatact
    16861 aacagtcgat atagattttc actcagcgct aaagatttca ttgatcgctg gtcaaaacaa
    16921 ggacccgccc atcattgtgc tattgggata gggcatattg ctgataaaat tgaaaaatta
    16981 ggaaaaattt taaatattga cgttgtgaaa atatgttaat ttcaagaatt ctaattcaaa
    17041 ttacctccta tgttaaaaaa aataatctca gtaactttta tagccatttt attgatggca
    17101 tgtaaacaca aatcctccga tagttttcaa tataaagttg gcatattagg tgctccttcg
    17161 tcgcctttcg tagaatggaa cgatcataat cttgaattaa tgaagaaact tgggttcaat
    17221 actatgcaac taaatattgc atggggatac cgcccagcag ataatgcgtt gaatctcgaa
    17281 gatgtattag tagtacctaa tgaattcaga ttaccagttg ataatgattc tacattaaac
    17341 aagaataatg gtggtttaaa tacttattta cattcttctg cacgtattgc taaacgcgct
    17401 gccgaactaa aacacaggat tgccatatgt aaaaaacatg agatgcgcac cctatttcat
    17461 ttcggagcac catatgtagc atatccgcct attgaacctc tttcgcaatg catctctgac
    17521 gaaaaaacaa tacgcagata cgaaaagctc attgaattgt ttgctgccca atttaaaggg
    17581 gttgatgact tattgcttta tacatacgat caaaatgcat ggctttgtag cgaatatggt
    17641 ccttgtgaaa aatgctttgg caaaccactc aacgaaagag tgccagtttt tgtcaatgca
    17701 attgcacgga aatggcatca actcaaccct aatggacggc tttggtggga accatgggaa
    17761 atttcagcag ggcaaacata taaaattatt aataagctgg atagtacctg tattggatta
    17821 gctgtacatt caaatattgc tgaagtacag atagcatttc ctgccgatag atggtacaaa
    17881 aatgtggtta atattgccaa tagacgttca ataccagtac ttgttgaagt ttggttagga
    17941 actgctactg aggaaattga accttatatt tatctgcctt caccattagc cacacttcgt
    18001 gctttaaaag cacttaacaa tgttggtaaa atagcaggca ttaaggaata ctatggtaat
    18061 atccctgata gagaagatcc aaaccttcgg atgtcttcta ttttttttaa aaatccaact
    18121 atttcggaag aagaagcact aaagaaatta tctcaacctt atggatatag caaaaaagtt
    18181 cagcaatatt ggagattgtc gtcagaggct attgaaatgt atccgtggga tataagctgg
    18241 tttgctcgcg aagtgggccg tagcgaccct catcattcac tcacagaggc aactttaaaa
    18301 ggggcatcgt gggagactcc aagttggcaa tcgaatcgca gagctgcatt tatgcgtaca
    18361 gttgaaaccg attatccaca tttctggatg cttgaagatg ctcaattgcg ttttgattgt
    18421 acagctcaaa aggccgaaga tgcacttaat gctggaattg cagcccgaaa tgatatacct
    18481 aaacgaatga tgaaagaatt tgattccagt ttagaggaac tcagaggttt caaacaacgt
    18541 tgtttagctt atacttttca tattcgcgaa agtaatatat gcaaaatgct ccgaagttca
    18601 ataaaatccg gaaaaaaacc gaaccctgta cttgttgttg aattaaagga actgctcaaa
    18661 aaagatctta aaaatcaagg ggaaaataat tgcctacaac aagcgattaa tttattagat
    18721 aaaaatatat atcagttttt acaggtgtat tttgtaaaac ctccaaagaa aaccaataca
    18781 cttgatatgt atttcaatga ttcaattcct gccaatgtta aaacagtatg gacaataacc
    18841 tcaaaataaa aaataatatg aatcaattcg catttaaaat gaaacttaaa cccggtatga
    18901 aagccgaata taaaaaagac atgatgaaat atggcctgaa atgaagttac ttcttaagaa
    18961 atgtggtatt tatgattata gcatttactt agacaaagaa actgatatct tatttgccat
    19021 tcagaaacaa ttaggaagca actcatccca aaatttaggt tcaaacccta ttgtcagaag
    19081 atggcgggtg tacatggccg atattattga gacgaatcct gataattcgc ttgtttcgaa
    19141 ggaactaata caaatgtttt atatggaata aattaacaat aactttttta taaatagttg
    19201 cattttaaaa ctacataaat tttttaattt cacataacac tactatatta tgtaaaacta
    19261 aatttaaaat atttataatt aataattaaa cgaattttta ttataacaat acttcgttca
    19321 attttattat aaacaattga taatcaataa tataaatgta taattcctgt taaaatattt
    19381 tagtaagtca ttgggaatca gtatctttat agataattca ataaaaaata aagaaatatg
    19441 acttccaaaa ctcacttttc atataaaatg atatacagtt caatttgcga acattgtttt
    19501 ctttcattaa agccattcaa taaatggcgt aaagatttta taaaagacgt tctttggctt
    19561 attttaagta ttcacg
//
LOCUS       DAOSAS010000016        19389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_1980_length_19389_cov_12.801593, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000016 DAOSAS010000000
VERSION     DAOSAS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19389
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1980_length_19389_cov_12.801593"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..274
                     /locus_tag="PLR72_01430"
     tRNA            185..274
                     /locus_tag="PLR72_01430"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:219..221,aa:Ser,seq:gga)
     gene            351..1175
                     /locus_tag="PLR72_01435"
     CDS             351..1175
                     /locus_tag="PLR72_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOK35905.1"
                     /translation="MKKVFAILSILAIISFGSTVNVSAQEVASQTDQAAVNNDTASAM
                     VTDTAQAVEEVQAPAPAEGGGMHKALKQKFIEGGPAWMAPIALCLIIGLALCIERIIY
                     LSLSSTDTKKLLADIDKAWSKGGVEAAKEVCRNTRGPVASIFYQGLSRFDEGVEVVEK
                     TVASYGGVQLGLLETNLTWISLFIAISPSLGFLGTVVGMIEAFDRIQQVGDISATIVA
                     SGIKVALLTTVFGLIVAIILQVFYNYILTLVEGMTNDMEDSSISLLDIIVKYTTKK"
     gene            1187..1666
                     /locus_tag="PLR72_01440"
     CDS             1187..1666
                     /locus_tag="PLR72_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35906.1"
                     /translation="MSKIVEKIPTIIAITLVSISVILVLLIYVGGSGESIYNSAGELM
                     SVPRFTDVLIYWTYFLVALTIVITLVMVGRTNVKSYIINPQSAIKSLIPIILFILVFV
                     LSWIFGSSEEVKIIGYDGNQNVGDWARFCDMMIYPVYVMLIALILTIIGAQIYKSLK"
     gene            1704..2309
                     /locus_tag="PLR72_01445"
     CDS             1704..2309
                     /locus_tag="PLR72_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOK35907.1"
                     /translation="MGKRKKRKVGQINASSMADISFLLLIFFLVTTSLNVGTGLARRL
                     PPPLPPDQKPPDIDIKKRNLFVVRINSKNQLMVQGEIIDIKDLRRKAKEFIKNENNDP
                     NLPELVTENIEYLGPMTFTKNHVISLQNDVGTTYQAYIDVQNELIAAYNELRDELAMQ
                     RFGKHYHELNEKLQGAIQKVYPQKISEAEPKNYGGVKSGTN"
     gene            2329..2766
                     /locus_tag="PLR72_01450"
     CDS             2329..2766
                     /locus_tag="PLR72_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOK35908.1"
                     /translation="MPALSTSSLPDIIFMLLFFFMAVTSLKEVTFKVRISPPLATEVQ
                     KLENKSLIRYIYVGKPLPQYQKIYGSETRIQLSDAFADVSEIAEYIVNERSAMKEEDQ
                     NLMTVCIKADRDTHMGIINDIKQALRRAYALKIIYAANPRPTS"
     gene            complement(3186..3785)
                     /gene="yihA"
                     /locus_tag="PLR72_01455"
     CDS             complement(3186..3785)
                     /gene="yihA"
                     /locus_tag="PLR72_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOK35909.1"
                     /translation="MEIKSATYVKSSENYLKCPEGNLPEYAFIGRSNVGKSSLINMLT
                     HRKKLTMTSSKPGKTLLINHFLINNQFYFVDLPGYGYSSLQKEKRKKLSKMIEDYILY
                     REQLVNLFVLLDVRLDPQKIDIEFIEWLGKNKVPFSLIFTKIDKLSTSQVNEKIQNYQ
                     NILLQQWEELPPVFLTSSLKQLGRKELLQYVENINKNLK"
     gene            complement(3819..5309)
                     /locus_tag="PLR72_01460"
     CDS             complement(3819..5309)
                     /locus_tag="PLR72_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOK35910.1"
                     /translation="MSGATILFIIAAYFSVLLLISFIVSRKSNNNDAFFLANRKSPWW
                     IIAIGMVGSSISGVSLVSVPGMVPSINFTYMQTVFGFFFGYLIIANVLLPLYYKLNLT
                     TIYTYLEERFGKHSYKTGASFFLLSKTIGAAARLYIVALILQNFVFEAWHIPFELTVC
                     GIIFMIWLYTQKSGIKTIVWTDTLQTIILITALVLIIVEVSKQLNMGFDGIVYQLINS
                     EKTRIFVFDDWHTKQNFFKQFFSGIFIAIVMTGLDQDMMQKNLTCRNLHDAKKNMYWY
                     GISFVPVNFLFLVLGAMLLLLAEQNNIVLPALGDEILPMFAVNYLGNTVLILFIIGIV
                     AAAFSSADSALAALTTSFSVDILGVQHQEEEKAIRTRRIIHIGISIVFALIIIIFKVI
                     NNKSVIDAIYTIASYTYGPLLGMYAFGLFTKLKVKDKAIPYIAILSPLICGTMNYFSM
                     RYISYAFGYELLMINGLLTFIGMWLFRIRDDEEIKLLNPHPIEIKK"
     gene            5451..6074
                     /gene="recR"
                     /locus_tag="PLR72_01465"
     CDS             5451..6074
                     /gene="recR"
                     /locus_tag="PLR72_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOK35911.1"
                     /translation="MGQEQFPSILVENAVFQFAKLPGIGRKTALRLTLYLLQQPEEVV
                     EFFGETLIRLRKEILYCKVCHNISDTEICSICNNPARDHQTICVVENIKDVMAIENTQ
                     QYKGVYHVLGGIISPMDGIGPNNLEIDSLIQRAKEENIKEIILALSTTMEGDTTNFYI
                     YRKLAPLKIKITTLARGVAIGDELEYADELTLGRSILNRVEFNPTLK"
     gene            6143..6586
                     /locus_tag="PLR72_01470"
     CDS             6143..6586
                     /locus_tag="PLR72_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35912.1"
                     /translation="MVEKIIKKLLYIYYGIFVGGVIIALLGFIFSQNHSPIPPQSNAG
                     ITLSSLLIIFVIGSIPLSLYFFNKKLSKWSVLENEELRLKKYEKGARLRLLIIGTGFI
                     IGILFFYVLRLQSMIFCAAIAAVALIFCKPHQQQMRNDLKLEDKE"
     gene            6664..>6965
                     /locus_tag="PLR72_01475"
     CDS             6664..>6965
                     /locus_tag="PLR72_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35913.1"
                     /translation="MIIAVDFDGTIVKHAYPKIGTEIPFAIEALKRLQTEYQHRIILW
                     TVRQGKELEEAVEYCRKRGLEFYAVNKNYPEETENDSQSRKIRADIYIDDRNLGGLP"
     assembly_gap    6966..7065
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7066..7250
                     /locus_tag="PLR72_01480"
     CDS             <7066..7250
                     /locus_tag="PLR72_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35914.1"
                     /translation="RKIRADIYIDDRNLGGLPDWGVIYQMIVTNNYLESSSQKNIKYN
                     KPSKKNIKSIFNKNKK"
     gene            complement(7360..7872)
                     /locus_tag="PLR72_01485"
     CDS             complement(7360..7872)
                     /locus_tag="PLR72_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOK35915.1"
                     /translation="MGKEAIKVASVDVNKLLEMLNAALSEEWLAYYQYWIGARLMEGP
                     MRSEIEPELLIHANEELSHAELIVDRIIQLGGTPVLNPIEWEKLARCTYEAPTDPYIE
                     VILEQNLRGERCAIDRYQEIASFTDGKDFTTHQMAITILNQELEHENDIEDWIKDIQL
                     MKDEFKKIRL"
     gene            8212..9687
                     /gene="guaB"
                     /locus_tag="PLR72_01490"
     CDS             8212..9687
                     /gene="guaB"
                     /locus_tag="PLR72_01490"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOK35916.1"
                     /translation="MSFIAEKIQFEGLTFDDVLLIPSYSEVLPREVDLSTRFSRHIEL
                     KNPIVSAAMDTVTESKMAIAIAREGGIGVIHKNMTIAEQAKQVEIVKRAENAMISNPV
                     TIPQGATVGDALKIMNEYKIGGIPVIDDGGYLVGIVTNRDLRFQRELNKKIDEVMTKE
                     NLITTSRTTTLEEAADILQQYKIEKLPVVDENNKLIGLITYKDITKAKDKPMASKDKE
                     GRLRVAAAVGVTPDVFDRIKALVAAEVDAIVIDTAHGHSKGVIETLKKTRQLFPEVDI
                     VVGNIATAEAAHDLVEAGADAVKVGIGPGSICTTRIIAGVGVPQLSAIYEVSKALKNT
                     NVPLIADGGIRYSGDIVKALAAGADTIMTGSLFAGVEESPGETIIFNGRKFKTYRGMG
                     SLEAMEKGSKDRYFQDMEDDIKKLVPEGIAGRVPYKGTLYEVIYQLIGGLRAGMGYCG
                     AKTITELHNAKFTRITNAGINESHPHDVTITSEAPNYSRSE"
     gene            9834..11771
                     /locus_tag="PLR72_01495"
     CDS             9834..11771
                     /locus_tag="PLR72_01495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOK35917.1"
                     /translation="MKLSFYKCAFFLLISSSLAAQVNDPVLMTINGNPVYKSEFEYIY
                     NKNNSNNFLDKKTLDEYVDLFINFKLKVEEAKAQGIDTTAAFINELKGYRAQLAKPYL
                     TDQKLEEALIKEAYDRMKEDVEVSHILVRIPQNATPADTLAAWNKIKLIQKRLEKEDF
                     AKVAREMSEDEMAEKTGGYIGWITVFQTPYSFESMAYNTPVGSISKPVRTSFGYHIIK
                     VHNRRNSPGEVQVAHIMRFTSNDPEKNKRAKEVIDSLYQRILAGDDFGTLAKNYSEDK
                     PSASKQGELPWFGTGRMIPEFEKAAFALKNIGDVSEPIQTPFGWHIIKLLDRRSLSSF
                     EKIKPDLERRLKYDERGKLPQQSFLKKLRAEYNYQVIPESVEEFYKLAEGHSLNDSIF
                     LVNISNLNKPLFSFADRKYTQQDLANYIKKNPYTQKTIPTEMINEKLNEFVDNELLNY
                     EDSQLEKKYDDFRLLMQEYHDGILLFEISNREVWEKAVQDTVGLANYFNQHKSDYKWS
                     KPHFKGLVIYCKDKNTFKTAKLIAKRSPEKEIEKNLLNRLNDSIQYVKIEKGLFVQGD
                     NKAVDKFIFKVKDNYTAPEGYPYVFVHGRLLKSPEDYTDVRGAIISDYQDYLEKEWIK
                     KLRTKYPFYVDKDVLKTVKEN"
     gene            11872..12732
                     /locus_tag="PLR72_01500"
     CDS             11872..12732
                     /locus_tag="PLR72_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOK35918.1"
                     /translation="MRLIYKIFILNLIVFFCSCLHKTHEISRQPLLQVEGNYLYLDEV
                     QQAIPANVAKKDSTEIANNFIKKWIIETLMYENAKRNISNQEEIEKLVEEYRKSLTIH
                     KYQQQLIQERLSRNIPEDEIKNFYEKYKEQLVAQESYIQGLLLIVPPTAPQIATVREW
                     VKSDNQKALENIEKYSLRNAIEYLYFGNKWMPFSEILKKIPLQINDPVSFLASNSFVE
                     LSDSTKHYFLKISAFVPAGKTMPYEIAKEDITHILLHKKEADFISQFESELYNDAMKK
                     KKIEFFKQTK"
     gene            12796..14154
                     /locus_tag="PLR72_01505"
     CDS             12796..14154
                     /locus_tag="PLR72_01505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOK35919.1"
                     /translation="MKKFFCLLNFLLLIFCLPNINAQKNLIDGIVWVVGDDAILRSEV
                     EEYKLRAQYEGVPIQGDPYCVIPEQLAIQKLFLHQAQLDSITVNESIVNNQVENRINY
                     FISQIGSKEKMEEYFGKSTTELRQDLREIIREQMIIQQMQQKLVGNINATPAEVRRFY
                     NQLPEDSIPMVPSQVELQIISYQPPIPQQEINRIKEQLLDFTRRVNNGEADFSVLARL
                     YSEDIESAKRGGELGFLGRGQLVPEFAEVAFNLQDPKKVSRIVETEFGFHIIQLIEKR
                     GDRINCRHILLKPHISEEDKQKAFHTMDSIANLIRNQKMTFEQAVIYYSHDKNTAMNA
                     GLMLNEKTGTSKFEYQDLPPEIAKVVYNMKVGEISDPFLMIDPKTNKEVVAIVKVKSK
                     IENHKANLRDDYLLIKNYYENKKRDEFLRNWIAKKQKETYIRIDPAWQNCEFQYPGWI
                     KK"
     gene            14243..16033
                     /locus_tag="PLR72_01510"
     CDS             14243..16033
                     /locus_tag="PLR72_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOK35920.1"
                     /translation="MYQYFVILFLFFIPFLALQAQNKPKQLKEEILKAQKESEKPPKV
                     KKKELTKPTTTLPPLKPVAPIANPLENKNATLIYLENSEIASFDKFQNPDIQVLKGNV
                     IFRHENALMYCDSAYFYPNSNSLDAFGNIRMVQGDTIFIYGNFLYYDGNTKLARLRKN
                     VRMENRKTVLTTDSMNYDRMMNLAYYYTGGKIQDELNTLTSIWGQYSPVTNEAIFKNK
                     VHLENENFIMDADTLKYNTKTNIANIVGKTHVIYKNETDIYSTRGWYNTANDRSMLLD
                     RSIVKQKNGKSLIGDTIFYDKRQKYGEAFQHVILKDTVEKTSLFGNYVSYNENSEKGL
                     ATDSALLVDWSSKDSLFLHADTLKTYKDSIYNMANGYYHVRFFRNDLQGVCDSIFYSG
                     RDSIIYLYGEPILWSEDYQITGDFVQAFLKNKKIDRVVIQLSAMAIQKHDSIHFNQLS
                     GKEIIAYLDSNQIKKIDIKGNAETLYYPIDSKDSTIIGQNKTQSSYVTVYMKDKKVDR
                     IVLTPASSGIIYPLEKLSEKDLYLKNYIWLPHLRPLKSTDVFLSSPYESRRKPHSSNA
                     EKNVSFSNAPNTNLEKKDNAIKGKKDTNLQ"
     gene            complement(16066..17403)
                     /gene="argH"
                     /locus_tag="PLR72_01515"
     CDS             complement(16066..17403)
                     /gene="argH"
                     /locus_tag="PLR72_01515"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOK35921.1"
                     /translation="MPKLWDKNSKQLNAKIEAFTIGEDQKWDGYLAKYDVIGTLAHIQ
                     MLCAVGLLKPEELELLRKELKSIYQEIEKENFKIEEGVEDVHSQIELMLTRKLGDVGK
                     KIHSARSRNDQILLDLKLFTRTEIEKIVHAVVELFDSFIAQSEKYKEVLMPGYTHLQV
                     AMPSSFGLWFGAYAESLADDLQLMLSAWKITNRNPLGSAAGYGTSFPIDRQMTTDLLG
                     FDSMNYNVIYAQMGRGKMERIVLSALASVAATLSKFSYDVCLYNSQNFGFIHLPDEFT
                     TGSSIMPHKKNPDVFELLRAKCNKIQSLPQQILTITNNLPSGYFRDFQIIKEIFIPAF
                     SELLNCLEIATMMVEKIEVNTHLLDDPRYDDLFSVEEVNRLVQAGEPFRDAYKKVGNE
                     IERGSFKPEKKIHHIHQGSIGNLCNDKIIAYKDEILSQFNFEKVHQAEKALLL"
     gene            complement(17450..17878)
                     /locus_tag="PLR72_01520"
     CDS             complement(17450..17878)
                     /locus_tag="PLR72_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOK35922.1"
                     /translation="MTNYESEAKTIFSNIEKVFNQLSDLTNLESLLDGNKSEVKDKLK
                     DLQFDADSFNFSIETFGKIGFRIIERKPFEIVKFQSEYFPVKMNVWILLNETAENETN
                     MKLSIEADIPLMIKVMVDSKLKEMVNLLADTISSALNMNN"
     gene            complement(17908..18546)
                     /gene="pyrE"
                     /locus_tag="PLR72_01525"
     CDS             complement(17908..18546)
                     /gene="pyrE"
                     /locus_tag="PLR72_01525"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOK35923.1"
                     /translation="MKTLEKNIAAALLQIKAVKLQPHNPFTWASGWRSPIYCDNRKTL
                     SYPSVRNYIKIELTRLIQEMYPEVEVIAGVATGAIAQGVLVADELSLPFIYVRTAPKD
                     HGLENLIEGDLRPRQKIVVIEDLISTGGSSLRAVEAIRNNGSQVLSIFSIFTYGFPMV
                     EEKFKTAKVKLTTLCNYKAIIQQALASKYISEEDYAILEDWHKDPAGWGNKI"
     gene            18975..19241
                     /locus_tag="PLR72_01530"
     CDS             18975..19241
                     /locus_tag="PLR72_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOK35924.1"
                     /translation="MEKTITFNELRRIKDSLPSGSITKIANELGLEEETVRNYFGGHN
                     FKNGKSCGIHIEPGPDGGIVMLDDTKILDIALRILEEQKEKKGK"
BASE COUNT         7019 a         2999 c         3077 g         6194 t
ORIGIN      
        1 gtattaaaaa aaccaatgga tgaagttgac cagcaaacga ccagaaatgc aaatgaaatt
       61 tttaaacttt tttatagaga ctcataaaac atcaaataaa aaatattttt tatgagaaaa
      121 gaataaaaat aaaaaataaa tgtgttgtat ttcatatttt tttttttaac ttgcacctca
      181 aaatggagag gtgctcgagt ggacgaagag gtgcgtctgg aaagcgtata tacggtgttg
      241 aatcgtatca agagttcgaa tctcttcctc tccgcaattc ttcatttaaa aagaattagt
      301 aattaataaa aataaaaaca ataaatttag aaattttaaa aacaaaaaca atgaaaaagg
      361 tatttgcaat cttatcaatt ttagcgatta tctcttttgg aagcacagta aatgtttcag
      421 ctcaagaagt tgcttctcaa acagaccagg cagcagtgaa caatgatact gcttcagcaa
      481 tggttacaga tacagctcag gctgtagaag aagtacaagc accagcacca gcagaaggtg
      541 gaggaatgca caaagcttta aaacagaaat ttattgaagg tggacctgca tggatggctc
      601 ctatagccct ctgcttaatt ataggattag ctttgtgtat tgaacgtatt atttatctaa
      661 gtctttcttc taccgatact aaaaaattgt tagccgacat agataaggcc tggagtaaag
      721 gtggtgttga ggctgcaaag gaagtatgtc gtaataccag aggacctgta gcctctattt
      781 tctatcaggg attgtcccgc ttcgatgaag gagttgaagt agtagaaaaa accgtagctt
      841 cttatggagg tgtacaattg ggtttattgg aaaccaatct tacatggatc agcttattta
      901 ttgctatttc accaagttta ggtttcttag gaacagttgt tggtatgatt gaggcttttg
      961 accggatcca acaagtagga gatatttcag caactattgt agcaagtggt attaaagtgg
     1021 cattgttaac aacagtgttt ggacttattg tagcaataat tctgcaagta ttttataatt
     1081 acattcttac tttggtagaa ggaatgacta acgatatgga agattcttct atctcattac
     1141 tggatatcat tgtaaaatat actactaaaa aataaataat aaagttatgt caaaaattgt
     1201 agaaaagata ccaactatca tagcaataac tttagtaagt atatctgtaa tattggtatt
     1261 attaatatat gtaggaggaa gcggtgaatc tatttataat agtgcgggtg aattaatgtc
     1321 ggttcctcgt tttacagatg ttctgatata ttggacatac ttcctcgttg ctttaacaat
     1381 tgttatcacc ttagttatgg tggggagaac caatgtaaaa agttatatta ttaatcccca
     1441 atcagccatt aaatctttaa ttccaatcat tctatttatt ttagtatttg tattatcctg
     1501 gatttttggc agctctgagg aagtaaaaat tattggttat gatggaaacc agaatgtagg
     1561 tgattgggca agattttgcg atatgatgat ttatcctgtt tatgtgatgc ttattgccct
     1621 catattaaca attattggtg cacaaattta taagagctta aaatagagca ttttttaaaa
     1681 tttattttaa attaaaaata aacatgggga aaagaaaaaa aagaaaagta gggcaaataa
     1741 atgccagttc tatggctgac atttcatttt tgcttttgat ttttttcctt gttaccactt
     1801 ccttgaatgt gggaacgggt ttggcaagac gtttaccgcc tcccttacca cctgatcaga
     1861 aaccaccaga tatagatatt aagaaacgta atctttttgt ggtgcgtata aatagtaaga
     1921 atcaattaat ggtacaagga gaaataatag atatcaaaga cctaagaaga aaggcaaaag
     1981 agtttatcaa aaacgaaaat aatgatccaa atcttccaga actcgtaact gagaatattg
     2041 aatatttagg tcccatgact tttacaaaaa atcatgttat ttctcttcaa aatgatgtag
     2101 gcacaactta ccaagcttat attgatgtac aaaatgagtt aatagctgct tacaatgaat
     2161 tacgagatga attagctatg cagcgtttcg gcaagcatta tcacgaattg aatgaaaagt
     2221 tacaaggagc aattcagaag gtttatcctc aaaagatatc tgaagctgaa cccaaaaatt
     2281 atggaggagt gaaaagtggc acgaattaga gaaaaagaaa aaagagaaat gccggctttg
     2341 agtacatctt cattgcctga tattattttt atgctgttat tcttctttat ggctgttact
     2401 tcattgaaag aggtgacctt taaagttaga attagtcctc ctcttgcaac tgaagtccaa
     2461 aagttagaaa acaagtcgtt gattcgttat atttatgtag ggaaaccgct accacaatac
     2521 caaaagattt atggatcaga aacacgtatt caactaagcg atgcttttgc tgacgtatct
     2581 gaaatagcag aatacattgt aaatgagcgg agtgctatga aagaagaaga tcaaaatttg
     2641 atgacagtat gtatcaaagc ggatagagat acacacatgg gtatcattaa tgatattaaa
     2701 caagctttac gaagagctta tgcgttgaaa attatttatg ctgccaatcc acgtccaaca
     2761 tcttaaacac tattgacaac ataaaaaata aatcgagtag gaggaaaata aatcatagtt
     2821 tcctcctatt ttattttccg atcttttcac ataaccgtaa agatggggct aatctaaaat
     2881 ttgctattaa gtgaaatttc taatggcaat attagaaaaa taatttataa ttatttgaat
     2941 tacaatataa tgtatcaacg aaaaattgca aaattgaggt tgttttttaa agctctgaaa
     3001 gtaggaaatt cttactcgtt aaagagtgaa acaaaatata agtacaaaaa tttttaatta
     3061 tttaaaattg gcttggcaat taaatatttg tttagcgtaa agtattatac ttatacaaaa
     3121 atccttattc cccgataata tgaaatcttg ctaactcgca ttccttacat cattacctat
     3181 aagaattatt tgagattttt attaatattt tccacatatt gaagcaattc tttcctacct
     3241 aattgtttta aggaagaagt taaaaatacc ggtggtaatt cttcccactg ttggagtaaa
     3301 atattttgat aattttgaat cttttcgttt acctgactag ttgataattt atcaatcttt
     3361 gtaaatatca atgaaaaagg gactttgttt tttccaagcc attcaataaa ttcgatgtcg
     3421 attttttgag ggtcgagtcg aacatcgagt aatacaaata aatttactaa ttgttcacga
     3481 taaagaatat agtcttcaat catttttgaa agtttttttc gcttttcttt ctgcaacgat
     3541 gagtaaccat atcctggcaa atcgacaaaa taaaattgat tgttaattaa aaaatgatta
     3601 attaacaaag tttttcctgg ttttgaggaa gtcatggtta attttttccg atgtgtcaac
     3661 atgttaatta aggatgattt accaacatta gaacgtccaa tgaaagcata ttcaggtaaa
     3721 tttccttcag gacacttcaa ataattttca ctacttttta catatgttgc cgatttaatt
     3781 tccatgtttt tttaatcaaa caataaaaaa tctgtcgatc actttttaat ttctatagga
     3841 tgaggattta aaagctttat ttcttcatca tcccgaatcc taaataacca catacctata
     3901 aaagttaaca acccatttat cataagcaac tcataaccaa aagcataaga tatatatcgc
     3961 atgctaaaat aattcattgt gccacaaatt aatggagata aaatagcaat ataaggaata
     4021 gctttatcct ttactttcaa tttagtaaaa agtccaaaag catacattcc cagcaaggga
     4081 ccataggtat aagaggcaat ggtataaatc gcatcaataa cacttttatt attaatgacc
     4141 ttgaaaataa taataatcaa agcaaacaca atagaaatgc caatatgaat aattcttctt
     4201 gtgcgaatag ctttttcctc ttcttgatgt tgaacgccta aaatgtccac agaaaaagag
     4261 gtagtaagag cagccagcgc tgaatcggca ctcgaaaaag ctgcagctac aattccaata
     4321 ataaacaata ttaaaactgt atttcctaaa taattgacag caaacatggg taatatctca
     4381 tctcccaatg ctggtaaaac gatattgttc tgttctgcaa gtagtaacaa cattgcacct
     4441 aaaactaaaa ataaaaaatt aaccggaaca aatgaaatcc cataccaata catatttttt
     4501 tttgcatcat gcaaatttcg acacgttaaa ttcttttgca tcatatcttg atcaagtcct
     4561 gtcataacaa tagcaataaa aataccgctg aaaaattgtt taaaaaaatt ctgcttagta
     4621 tgccaatcat caaaaacaaa aattcgtgtt ttttcactat taatcaattg ataaacgatt
     4681 ccatcaaatc ccatgttcaa ttgttttgaa acctctacaa taattaatac caaggctgtt
     4741 attaaaataa tggtttgaag cgtatctgtc cacacaatag ttttaatccc acttttttga
     4801 gtatataacc aaatcataaa aataatacca caaacagtca attcaaaggg tatatgccac
     4861 gcttcaaaaa caaaattttg caaaattaag gctacaatat acaagcgagc cgcagctccg
     4921 atagttttcg ataataagaa aaaagaagct cctgttttat aagaatgttt accaaaacgt
     4981 tcttctaaat aggtatagat agtagtcaag tttaatttat aatacaaagg taaaagcaca
     5041 tttgcaataa tcagataacc aaaaaagaaa ccaaaaacag tttgcatata agtaaaattg
     5101 atacttggca ccatcccagg aacagaaaca agactaactc ctgaaattga tgaacctacc
     5161 attccaatag caattatcca ccagggtgat tttcggtttg ctaaaaagaa agcatcatta
     5221 ttgttgcttt ttctactgac aataaacgaa ataagtaata aaactgaaaa ataagcagca
     5281 ataataaaca aaatagtagc acctgacatt ttttaattct atttattaat taaatttttt
     5341 tgaatttgac aaaattagtg aaaaaatctg aaggattaaa tctataggag tgttgattcg
     5401 ccaactaaat ttttgtaaat ttgcaataat aattgtattt ataattgatt atgggacaag
     5461 aacaatttcc ttcaatacta gtggaaaatg ctgtgtttca atttgctaaa ctaccgggca
     5521 ttggaagaaa aacagctctc aggctgactc tttatcttct tcagcaacct gaagaagtag
     5581 tagaattttt tggagaaaca ctgattcgct tgcgtaaaga aattctttac tgcaaagttt
     5641 gtcacaatat ctcagataca gaaatttgtt ctatttgcaa taatcctgcc agagaccacc
     5701 aaaccatttg tgtagtagaa aacataaaag atgtgatggc aatcgagaat actcagcaat
     5761 ataaaggagt ttatcacgtg ctgggcggta ttatttcacc tatggacggc attggaccaa
     5821 acaacttgga aattgatagc ttaattcaaa gagctaaaga agaaaacatt aaggaaatta
     5881 ttctcgcact aagtactaca atggaaggcg atactaccaa tttttatatt tatcgtaaat
     5941 tagcccctct aaaaataaaa atcactactt tagcacgtgg agttgccatt ggagatgaat
     6001 tggaatatgc ggatgaattg acattgggac gctccattct aaatcgtgtt gaatttaatc
     6061 caactcttaa ataagatttt actataaaat actaataatc aatataataa gttattttta
     6121 cactcatttt aacagccaaa aaatggtcga aaaaattatc aaaaaattac tatatatata
     6181 ctatggaatt tttgttggtg gagtaataat agccttgctt ggttttattt tttcccagaa
     6241 ccactcccct attccacctc aaagtaatgc aggaattacc ttatccagct tattgattat
     6301 atttgttatt ggttccattc ctctgagttt atattttttc aataaaaaat taagtaaatg
     6361 gtctgtgctt gaaaatgaag aacttagact aaagaaatac gaaaaagggg ctcgattaag
     6421 attgctcatt attggtacag gattcataat tggtatctta tttttttatg tacttcgttt
     6481 gcaatcaatg attttttgtg ctgctattgc tgctgttgct cttatttttt gtaagcctca
     6541 tcagcaacaa atgaggaatg atttaaaatt agaagataaa gaataacttt attaaaaaga
     6601 accagcttct taacttctaa gggaaattta tttgtaaaat aactattcaa aaaaattcag
     6661 gtaatgatta ttgcagttga ttttgatggc acaattgtta agcatgctta tcccaaaatt
     6721 ggcacagaaa tcccttttgc catagaggca ctaaaacgtc tgcaaactga atatcagcat
     6781 cgaattattt tatggacagt tcgccaggga aaagaactgg aagaagcagt tgagtattgt
     6841 cgtaaacgcg gactagaatt ttatgcggtt aataaaaatt atcctgaaga gacagaaaac
     6901 gattctcaat cgagaaaaat cagagctgac atttatattg atgatcgcaa tttaggagga
     6961 ttgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgaga aaaatcagag
     7081 ctgacattta tattgatgat cgcaatttag gaggattgcc ggattgggga gtgatttatc
     7141 agatgattgt tacaaataat tatcttgaat cctcttctca gaaaaatatt aaatacaata
     7201 aaccttcaaa aaaaaatatc aaatcaattt tcaataaaaa taaaaaataa cgtaaaaaaa
     7261 ttttttattt caacataaaa aaccattata aattagttat attttttggt tataaaaaac
     7321 caacttataa tggttttact ttcaatagaa tttttaaaat cagagtctta tctttttaaa
     7381 ttcatctttc atgagttgaa tatcttttat ccaatcttca atatcattct catgttcaag
     7441 ctcttgattt aaaatagtaa ttgccatttg atgagtagtg aaatccttcc catctgtaaa
     7501 actggcaatt tcttgataac ggtcgatagc acaacgttct cctcttaaat tttgttccag
     7561 aatcacttca atatacggat cggttggtgc ttcataagta caacgtgcta atttctccca
     7621 ttctattgga tttaaaactg gggtaccacc taactgaata attctgtcaa cgattaattc
     7681 tgcatgactt aattcttcat tcgcatgaat taaaagttcg ggctcgattt cacttctcat
     7741 tggaccttcc ataagcctgg cgccaatcca gtattgataa tatgccaacc attcttcgga
     7801 aagtgcagca tttaacattt caagtaactt gttgacatct acagatgcaa cttttatagc
     7861 ttctttcccc ataacaaaca atttttaaag gttaatgaat tcttttaaaa aatttattca
     7921 ttcgacacaa gcaaaagtaa taattttttc tcaatttgaa aattattctt gtgtaaaaat
     7981 aaggaattat ttgaaagaaa aacaaatttt gaattatacg tattttttgc taatttttat
     8041 gtaaatttgc gtgcaataat tttcatacgt ttattattat atttttatct caaggtgata
     8101 taaaacttgt caataaaata tatatacttg tgaataagtt attttatccg acttgtttta
     8161 tggaatattt tccttgaaaa catttttcaa aaaccataaa aaattaaaac tatgtcattt
     8221 attgcagaaa aaattcaatt tgaaggttta accttcgatg atgtgttatt aattccttct
     8281 tattcggaag ttttaccacg cgaagttgat ttatccactc gtttttctcg tcatatagaa
     8341 ctaaaaaatc ctattgtttc tgctgctatg gacaccgtta cggaatcgaa aatggcaatt
     8401 gccatagctc gagaaggcgg tattggggtg attcacaaaa atatgaccat tgctgaacag
     8461 gcaaagcagg tagaaatagt taaacgtgcg gaaaatgcaa tgatttccaa tcctgtcacc
     8521 attcctcaag gtgcaacggt aggagatgca ttgaaaataa tgaacgaata taaaattgga
     8581 ggaatacctg ttattgacga tgggggctat ttagtaggaa ttgtaactaa tcgtgattta
     8641 cgttttcaac gagaattgaa taaaaaaatc gatgaagtaa tgaccaaaga aaatcttatt
     8701 actacttcac gaactaccac attagaggaa gctgctgata tcctccagca atataaaatt
     8761 gagaaacttc cagttgtaga tgaaaacaac aaattaatcg gattaataac ttataaagat
     8821 attaccaaag caaaagacaa gccaatggct tcgaaagata aagaaggaag attacgtgtg
     8881 gctgctgcgg taggagtaac tcctgatgtg tttgatcgaa taaaagccct tgtagcggct
     8941 gaagttgatg ccattgtgat tgataccgct catggacatt caaagggagt gattgaaact
     9001 ttaaaaaaaa cacgtcaatt atttccggag gttgatatag tagtaggcaa tatcgccact
     9061 gctgaagctg cccatgattt agtggaagct ggagctgacg cagtaaaagt cggtattgga
     9121 ccaggttcta tttgtaccac tcgaattatt gcaggcgtag gagtccccca attatctgct
     9181 atttatgaag tttctaaagc attaaaaaat accaatgtac cattaattgc agatggagga
     9241 atcaggtatt caggcgatat tgtaaaagct ctggctgctg gtgccgatac tattatgaca
     9301 ggttcattat ttgccggtgt tgaagaatcg ccgggtgaaa ccataatttt caatggacgt
     9361 aaatttaaga cttatcgtgg aatgggttct ttggaagcaa tggaaaaagg ttcaaaagat
     9421 agatattttc aggacatgga agacgatata aaaaaattag ttcctgaagg aattgctgga
     9481 cgagttccct ataaaggtac actttacgaa gtaatttatc agttaatcgg aggattgcga
     9541 gctggaatgg gttattgtgg cgctaaaact attacagagc ttcataatgc caaatttact
     9601 cgaatcacaa acgctggcat caacgaaagc catccacacg atgtaaccat taccagcgaa
     9661 gcaccaaatt acagcagatc ggaataaaat aaaacttcaa atgctgaaaa aatctttctg
     9721 atattacaac ataatgaata atagtacgtt tatcaaagta aaagaaaatg ttaactaata
     9781 attataaatt gctttacgaa aaaatcataa ccaataacta aaataatgta ccaatgaaat
     9841 tatcttttta taaatgtgcc ttttttctct tgatctcaag cagtttggct gcacaagtga
     9901 acgatccagt cttgatgact ataaatggta atccagttta taaatcggaa tttgaatata
     9961 tttataataa aaataactca aataattttt tagataaaaa gacattagat gaatatgtag
    10021 atttatttat taatttcaaa ttgaaagttg aagaagcaaa agctcaggga attgatacca
    10081 ccgctgcttt tatcaacgaa ttaaaaggtt atcgagccca actggcaaaa ccttatctga
    10141 cagatcaaaa gttggaagaa gctttaataa aggaagctta cgaccgtatg aaagaagacg
    10201 tggaagtaag tcatattctg gtcagaattc ctcaaaatgc tactcctgct gatacattgg
    10261 cagcatggaa caaaataaaa ttgattcaaa aacgattaga aaaagaagat tttgcaaaag
    10321 ttgccagaga aatgtcggaa gacgaaatgg ctgagaaaac aggtggctat attggatgga
    10381 taactgtttt tcagacgccc tattcttttg aaagtatggc ttataatacg cccgttggtt
    10441 ctatttcaaa gcctgtacgt acttcttttg gctaccatat tattaaggtt cacaatcgga
    10501 gaaattctcc aggcgaagtg caggttgcac acattatgcg gtttacttcc aatgatcctg
    10561 aaaaaaataa acgtgctaaa gaagtgattg attctctata tcaaagaatt ttagcaggag
    10621 atgattttgg aactttagca aaaaactatt ctgaagataa accttctgct tcaaagcaag
    10681 gagaattacc ctggtttgga acaggaagaa tgattcctga atttgaaaaa gcagcatttg
    10741 ccttaaaaaa tattggtgat gtttcagaac ctattcaaac gccttttggt tggcatatta
    10801 ttaaattatt agaccgaaga agtttgtctt cctttgaaaa aataaaaccc gatttagaaa
    10861 gacggctcaa atatgatgaa agaggcaaat tgccacaaca gtcatttctg aaaaaattaa
    10921 gggctgaata caattaccaa gttattcctg aaagcgtaga agaattttat aaattagctg
    10981 aaggtcattc tttaaatgat tcaatctttt tggttaacat tagtaactta aataaaccat
    11041 tgtttagttt tgctgacaga aaatatacac agcaggattt agctaactat atcaaaaaaa
    11101 atccttacac tcaaaaaaca attccaacag aaatgatcaa tgaaaaattg aatgaatttg
    11161 ttgataatga attgttaaat tatgaagata gccaattaga aaaaaaatac gatgatttcc
    11221 gcttgttaat gcaggaatat catgatggaa ttcttttgtt tgaaatcagc aatcgtgaag
    11281 tgtgggaaaa agcagttcaa gatactgtgg gtctggctaa ttattttaac cagcataaat
    11341 cagattataa atggtcaaaa ccccatttca aagggctggt aatttactgt aaggacaaaa
    11401 atacttttaa aacagctaaa cttattgcaa aacgctcacc tgaaaaagaa atagaaaaga
    11461 atttgctaaa tcgattaaat gacagtattc aatatgtaaa aattgaaaaa ggtttgtttg
    11521 ttcaaggtga taataaagca gtagataaat ttatttttaa ggtaaaagat aattatactg
    11581 ctcctgaagg ttatccttat gtctttgttc atggacgttt acttaaatct ccagaagatt
    11641 atactgatgt tcgcggagcc attataagtg attatcagga ttatcttgaa aaagaatgga
    11701 tcaaaaagtt aagaaccaaa tatccttttt atgtggacaa agatgttttg aaaacagtta
    11761 aggaaaacta ataaatttta tcaaagttct tcaacaacaa aataaaagct aacttgcata
    11821 gcttttattt tgttgtttat aagagtcggt ttatctctta gtaaaaactt catgaggtta
    11881 atttataaaa tttttatttt aaatttaata gtcttttttt gttcctgttt acataaaact
    11941 cacgaaataa gtcgtcagcc ccttcttcag gttgaaggca attatttata tctggatgaa
    12001 gttcaacagg caattcctgc taatgttgct aaaaaagaca gtactgaaat agctaataac
    12061 ttcataaaaa aatggataat tgaaactttg atgtatgaaa acgccaaacg aaatattagc
    12121 aatcaagaag aaattgaaaa gctggtagaa gaatatcgaa agtcgctcac aatacataaa
    12181 tatcaacaac aattgattca ggaaagatta tccagaaata taccagaaga tgaaattaag
    12241 aatttttatg aaaaatacaa agaacaatta gtagctcaag aaagttatat acaaggatta
    12301 ttgctaatag ttccaccaac agctcctcaa atagctacgg tacgggaatg ggttaaatct
    12361 gacaatcaaa aagcactgga aaatattgaa aaatatagtt tgcgtaatgc tattgaatat
    12421 ctttattttg gaaacaaatg gatgccattt tctgagattt tgaagaaaat tcctttgcaa
    12481 attaacgatc ctgtcagttt tttagcttcc aactcatttg ttgagttatc agacagcaca
    12541 aaacattatt tcttgaaaat aagtgctttt gtccctgcag gaaaaacaat gccttacgaa
    12601 atagcgaaag aagatataac ccatatcctg ctccacaaaa aagaagctga ttttatttct
    12661 caatttgaat cagagttgta taacgatgcg atgaaaaaga aaaaaattga atttttcaaa
    12721 caaacaaaat aattttataa aatcctaatt attaaaaaaa cattttattt aaaagctaaa
    12781 atcaaatcaa ttaaaatgaa aaaatttttt tgtcttctta attttttatt gttaatattt
    12841 tgcttaccaa atataaatgc ccaaaaaaat ttaattgatg gtattgtgtg ggttgttggt
    12901 gatgatgcta ttttgcgttc agaggttgaa gaatataaat tgcgtgctca atatgaaggt
    12961 gtacccattc aaggagatcc ttattgtgtc attccagaac agcttgctat tcaaaaactt
    13021 ttcttgcacc aggcccaatt ggatagcatc acagtaaacg aaagtatcgt caataatcag
    13081 gtagaaaatc gaataaatta tttcatttca caaataggat ccaaagaaaa aatggaagaa
    13141 tactttggaa aaagtacaac agaattgcgt caggatcttc gagaaataat tcgtgagcaa
    13201 atgattattc aacaaatgca gcaaaaacta gttggcaata ttaatgcgac tcctgctgaa
    13261 gtacgacgtt tttataatca attgcctgaa gatagtattc caatggtacc ttcacaagtt
    13321 gaattgcaaa ttataagtta tcaaccaccc attcctcaac aggaaatcaa ccgtataaaa
    13381 gaacaattat tggattttac aagacgagtt aataatggtg aagcagattt ctcagtcctg
    13441 gcaagactgt attctgaaga tatagaaagt gcaaaacgtg gaggtgaatt aggctttctg
    13501 ggacgaggac aattggtgcc tgaatttgca gaagtagcct tcaatctcca agatcccaaa
    13561 aaagtgtcac gaattgtaga aactgaattt ggatttcata ttattcaatt gattgaaaaa
    13621 cgaggcgacc gtattaattg tcggcatatc ctgctaaaac cacacatttc tgaagaagat
    13681 aaacagaaag cttttcatac aatggattca attgccaatt tgattcgtaa tcaaaaaatg
    13741 acttttgaac aagctgtcat ttattattct cacgataaaa atacagcaat gaatgcaggt
    13801 ttgatgttga atgagaaaac aggcacttca aaatttgaat atcaagattt acctcccgaa
    13861 attgcgaaag tcgtttacaa catgaaagtg ggtgaaattt cggatccttt tttaatgatt
    13921 gatcctaaaa ccaataaaga agtcgttgcc attgtaaaag tcaaatcaaa aattgaaaat
    13981 cataaagcca atttacgtga cgattattta ttgataaaaa attattacga aaacaaaaaa
    14041 agagacgagt ttttgcgaaa ctggattgct aaaaaacaaa aggaaactta tattagaatt
    14101 gatccagcat ggcaaaactg tgaattccaa tatccaggtt ggataaaaaa atgatttatc
    14161 ctgccaaatt caataatttt acatgatttc gtccattttt aaaaaaatat ttaattcttg
    14221 ttttttagct aacaaaagaa ttgtgtatca atactttgtt attctttttt tgttttttat
    14281 tccttttctc gctttacaag ctcaaaataa gcctaaacaa ttaaaagaag aaattttaaa
    14341 agcacaaaaa gaatcagaaa aaccaccaaa agtcaaaaag aaagaattaa ccaagcctac
    14401 aacaaccttg cccccattaa agccagtagc tccgatagca aaccctctgg aaaataaaaa
    14461 tgctacgtta atttatctgg aaaattctga aatagccagt tttgataaat ttcagaatcc
    14521 tgatattcag gttttaaaag gaaatgttat ttttcgacat gaaaacgcat tgatgtattg
    14581 cgatagtgcc tatttttatc ccaattcaaa ttcattggat gcttttggta atattcgcat
    14641 ggtgcaaggt gatactatat ttatatatgg taatttctta tattacgatg gaaatactaa
    14701 acttgcgcgt ttaagaaaaa atgttcgcat ggaaaatcgg aaaacagttt tgactactga
    14761 tagtatgaat tatgatcgga tgatgaattt ggcttattat tataccggcg gaaaaattca
    14821 ggatgaatta aatactttaa cttctatttg gggacaatat tcgcctgtca ccaatgaagc
    14881 catcttcaaa aacaaagtac atctggaaaa tgaaaatttt ataatggatg ctgatacttt
    14941 aaaatacaat accaaaacaa atattgccaa tatagtaggt aaaacgcacg taatctacaa
    15001 gaatgagaca gatatttatt caaccagagg atggtataat acagcaaatg atcgttctat
    15061 gcttttagac cgctctatag tgaaacaaaa aaatggaaaa tcactcatag gagataccat
    15121 tttttatgat aaaagacaaa aatatggaga agcttttcaa catgttattt taaaagatac
    15181 agtagaaaaa acttcacttt ttggaaatta tgtttcttat aatgaaaatt cagaaaaagg
    15241 attagctacc gattctgctt tactggttga ctggtcatcg aaagattctc tttttctaca
    15301 tgctgataca ttaaaaactt ataaggattc aatctataac atggctaatg gctattatca
    15361 tgttcgtttt ttcagaaatg atcttcaagg ggtttgcgat tctatatttt attctgggcg
    15421 agattcaatt atttatttat acggagaacc gattctctgg tcagaagatt atcaaatcac
    15481 aggcgacttt gtacaagctt ttttgaaaaa taaaaaaata gatcgtgttg ttattcaact
    15541 atcagcaatg gctattcaaa agcatgattc cattcatttc aatcaacttt cagggaaaga
    15601 aatcatagct tatcttgata gcaatcaaat taaaaaaatt gatatcaaag ggaatgcaga
    15661 aacactttat tatcctattg acagtaaaga ttctaccata atcggacaga ataaaacaca
    15721 aagcagttac gtaaccgttt atatgaaaga taaaaaagtg gatagaattg tgttgacacc
    15781 cgcttcttca ggaataattt atccattaga aaaactttca gaaaaagatt tatatttgaa
    15841 aaactatatc tggctacctc atttaagacc actgaaaagc actgatgtat ttttgtcaag
    15901 tccttatgaa tcacgcagaa agcctcattc aagcaatgca gaaaaaaatg tttctttttc
    15961 caatgcccct aatacaaatt tagaaaagaa agataatgct ataaagggaa aaaaagacac
    16021 caatcttcaa taattctatt gtgctaaata tcaaaaagat attttttaca ataacaatgc
    16081 tttctccgct tgatgaactt tttcaaagtt gaattgtgat aaaatctcgt ccttgtaagc
    16141 aataatttta tcattacata aattaccgat acttccctgg tgaatatgat gaatcttttt
    16201 ttcaggttta aaactgcctc tttcaatttc atttcctact ttcttataag catcacggaa
    16261 aggttctcct gcctgtacca aacgatttac ttcttcaacg ctaaaaagat catcgtaacg
    16321 tggatcgtct aagagatgag tattcacttc tattttttct accatcattg ttgctatttc
    16381 taaacaattc aacaattcac taaaagcagg aataaaaatc tctttaatta tttgaaaatc
    16441 acgaaaatag ccagaaggta aattattagt aatggtcaaa atttgttgag gaagtgattg
    16501 aattttatta cattttgctc ttaataattc aaagacatct ggattttttt tatgtggcat
    16561 gatgctggag cctgttgtaa attcatccgg caaatggatg aatccaaaat tctgagaatt
    16621 gtataagcat acatcgtaag aaaattttga aagagttgct gccacgctgg caagagcaga
    16681 aagaacaatc cgttccattt ttcctcttcc catttgagca taaattacat tgtaattcat
    16741 actatcaaat cctaataaat cagtggtcat ttgcctatcg ataggaaaag aagtaccata
    16801 acctgcagca gaaccaagcg gattccgatt agtgattttc catgctgata acataagctg
    16861 taggtcatct gccaaacttt cggcataagc tccaaaccac aagccaaaag aagaaggcat
    16921 ggcaacttgt aaatgcgtgt agcccggcat taaaacttcc ttatattttt cactttgtgc
    16981 tataaatgaa tcgaataact caacaacagc gtgtacgatt ttttctattt cagtacgagt
    17041 aaaaagtttc aaatccaata aaatttgatc atttctggaa cgggcactgt gaattttttt
    17101 cccaacatct ccaagtttgc gagtcaacat taattctatt tgtgaatgaa catcctctac
    17161 cccttcttcg atcttaaagt tttctttttc aatttcttga taaatacttt ttaactcttt
    17221 tcttaataat tccaattctt ctggtttaag taaaccaaca gcacatagca tctgaatatg
    17281 agccaaagta cctatcacat catattttgc cagataacca tcccattttt gatcttcacc
    17341 aatggtgaaa gcttctattt tggcatttaa ctgttttgaa tttttatccc aaagttttgg
    17401 catatataat aattgtatta ctaaataact ataattaatt ttgaataagt taattgttca
    17461 tatttagtgc tgaactaatc gtatctgcca gcaaatttac catttctttc agtttgctat
    17521 ccaccatcac tttaatcatt aaaggaatat cagcttcgat acttaacttc atatttgttt
    17581 cattttctgc agtttcattt aatagtatcc atacattcat tttcacagga aaatattcag
    17641 actgaaactt tacaatttca aaaggttttc tttcaataat tctaaagcca attttaccaa
    17701 aagtctcaat ggaaaaatta aaactgtctg catcaaattg caaatctttt aatttatctt
    17761 ttacttcaga tttgttacca tctaacaaac tttctaaatt tgtcagatcg ctcaactgat
    17821 taaaaacctt ttcaatattt gaaaaaatgg tttttgcttc actttcgtag ttcgtcattc
    17881 aatttatttt tttatgaaat tatctaatca tattttattt ccccaaccgg ctggatcttt
    17941 atgccaatct tccaatattg cataatcttc ctcagaaata tatttactgg ccaaagcttg
    18001 ttgaataatg gctttataat tgcacaaagt ggttagtttt acttttgcag tcttaaattt
    18061 ttcttcaacc attggaaatc cgtaagtaaa aatagaaaat atactcaaca cttgcgaacc
    18121 attatttcga attgcttcca cagctcttag actacttccc ccggtagaaa tcaaatcttc
    18181 tattactact attttctgac gtggacgcaa atctccttca attaaatttt ccaagccgtg
    18241 atctttgggt gcggtacgaa catagatgaa aggcaaactt aattcatcag ccaccagcac
    18301 accttgagcg atggctccag tagctactcc agctatcact tcaacttcag gatacatttc
    18361 ctgaattaaa cgagtcaatt caattttgat ataattccta acggaaggat aagaaagtgt
    18421 tttacggtta tcacaataaa tcggtgatct ccagccagat gcccacgtga atggattatg
    18481 aggctgcaat ttaactgctt ttatttgtaa aagtgctgct gctatgtttt tttctaatgt
    18541 tttcatctcg tatttataag ataatgaaat gattataatc ctttgaagct ataattttat
    18601 tttttacaaa tttaagcaaa gtatttaaga atttaagggt aaaaaatatt ctttataaca
    18661 tatttattat tttgatgaca ggaaaaataa aacaaatatt tttccaaaga ttaaaatgat
    18721 ttcaaatgaa aaaataaatt ctacatcgga caataaaatt tgttaaagat agtaaagtta
    18781 taaaacaaaa taaataaatg aattactatt tattttattg attaacaata acttaatttt
    18841 ttttacattt atgcaaaatt attatgttat aaaaattcaa aaaagttttg cttttaaaaa
    18901 agaatgtact taaatttaaa gcggataaaa aaatttggta tccactaata atcatctaaa
    18961 aatattttat tgacatggaa aaaacaataa cattcaacga gcttcgtaga atcaaagatt
    19021 ctcttccctc cggtagcatc acaaaaattg caaatgaact tggtttggaa gaagaaactg
    19081 ttcggaacta ttttggcggt cataatttta agaacggaaa aagttgtggc attcatattg
    19141 agccaggtcc tgatggtgga atagtcatgc tggatgatac gaaaatttta gatattgctt
    19201 tacgcatttt agaagaacaa aaagaaaaaa aaggcaaata aagaaacaaa aagcagtgaa
    19261 taatttcact gctttttttt aataacaaac aggcataatc agaaaaaata tcattcaatt
    19321 tttctatcaa taattttaat gattatccgc aagaagacct attataaatt cttgatctga
    19381 aatcggagt
//
LOCUS       DAOSAS010000017        18994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_2026_length_18994_cov_13.329479, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000017 DAOSAS010000000
VERSION     DAOSAS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18994
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2026_length_18994_cov_13.329479"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1249)
                     /locus_tag="PLR72_01535"
     CDS             complement(23..1249)
                     /locus_tag="PLR72_01535"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOK35925.1"
                     /translation="MNKIKVNQPIVELDGDEMARVMWKMIKEQLILPYLDLNIQYYDL
                     SIENRDATDDQVTIDAAYAIKKYNVGIKCATITPDEARIKEFGLKKMWKSPNGTIRNI
                     LGGTVFREPIICSNVPRLVPNWEKPIIIGRHAFGDQYKATDFVTKGKGKLKVTFTNEN
                     GESQEWEVYNFNGDGVALTMYNTDESIYGFARACFQAALEKKLPLYFSTKNTILKAYD
                     GRFKDIFQEVYEAEFKDKFQEIGITYEHRLIDDMVASVLKWKGDFIWACKNYDGDVQS
                     DAIAQGFGSLGLMSSYLLTPDGKTMEAEAAHGTVTRHFRLYQQGKETSTNPIASIFAW
                     TRGLSHRGKLDGNQELIDFCHELEQVCIETVESSKMTKDLALLVYGDKITRQDYLSTE
                     EFLSALKENLDKKLSN"
     gene            complement(1280..2635)
                     /locus_tag="PLR72_01540"
     CDS             complement(1280..2635)
                     /locus_tag="PLR72_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOK35926.1"
                     /translation="MKEEFLIYKLSETIKETSKIDKELFTKYNVKRGLRNEDHTGVLV
                     GLTRIGDVVGYKRTETGELKPIPGKLYYRGIDVEELVNGIQKENRLGFEETAYLLLSG
                     YLPNNEELETFSHIIYEKMPLEHQATISILNLKGHNIMNILARSVLELYIYDENPDNI
                     SRDNLIKQSLNLIAKFPTIISYAYNVLRHHEKGRSLHVRHPNENFSFAENFLYMIKGK
                     DQYTPLEVQILDLALILHAEHGGGNNSTFSVRVTSSTETDTYSSIAAGIGSLKGPLHG
                     GANLKVMGMIDDMKKNIADWTNPEEIDKYLLKILNKQAYDKSGLIYGVGHAVYTISDP
                     RAVLLKKMARELAKEKGLEKEFNFLELIEERAIKTFMEYKGKDVNKQVCINVDFYSGF
                     VYQAIGLPQEVYTPIFAMARVVGWCAHRIEELNFESKRIIRPAYKNVNENQEYIPLSK
                     R"
     gene            complement(2695..4965)
                     /locus_tag="PLR72_01545"
     CDS             complement(2695..4965)
                     /locus_tag="PLR72_01545"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOK35927.1"
                     /translation="MISNEEIFQQFYATFSIKIEKIQSKLRHPLTLTEKILYTHLAPN
                     ETIKNFRRGIDYVNFIPDRVAMQDATAQMALLQLMNSGREKVAVPTSVHCDHLIEANI
                     SAVEDLKKAKEINQEIYDFLKVVSNNYGIYFWEPGAGIIHQIIFENYAFPGGMMIGTD
                     SHTPTAGGLSMIAIGVGGADAVDVMAGLTWELRMPKIIGVKLSGKLSGWASPKDVILK
                     LAGILTVKGATNAVLEYFGEGTETLSATGKATICNMGAEVGATTSIFPFDKKSEQYLN
                     ATGRNSIAKIASELSNYLTADSEVLAHPEDFYDKVIEINLNEIEPHINGPFTPDAAYT
                     ISKFAKIVKEKNYPQKMEVGLIGSCTNSSYEDLSRAASVFEQAIEKKLKINAELLINP
                     GSEQIRTTAKRDGILEVFTKVGGVIMANACGPCIGQWNRKLIPADRPNSIVTTFNRNF
                     AKRNDGNPNTHAFLASPEIVAALTIAGDLCFNPLIDFLVNEKGEKVKLSEPIGFELPL
                     VGYETKEKGYIAPTGEKLPINIDPHSERLQLLQPFPAWDGKDMIELALLIKVKGKCTT
                     DHISMAGPWLRYRGHLDKISDNLLMGAVNAFNNKTNSVLYPPTGEYMPVSAAARRYKA
                     ENIGSVVVAEENYGEGSSREHAAMEPRYLNVKAIIAKSFARIHETNLKKQGMLALTFI
                     NKSDYDRIREDDKISILGLTEFSPGKNLKIQLHHSDGTTEQFEVAHSYNQTQIEWFKA
                     GSALNFIKEQFEKGNN"
     gene            5371..5847
                     /locus_tag="PLR72_01550"
     CDS             5371..5847
                     /locus_tag="PLR72_01550"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOK35928.1"
                     /translation="MKRQLKIALVAHDNRKVDMVEWALYNAEMLSQHKLFCTGTTGKL
                     IKKAMAERGIEVDITCMHSGPLGGDAEIAALVVQKKIDLAVFLIDDLNPQPHEADIQM
                     LLRQCRIHNVPLACNRYSADLMITSTLWDDETYVPTEPKYVKFVREDFFNSDEDLD"
     gene            6421..6684
                     /locus_tag="PLR72_01555"
     CDS             6421..6684
                     /locus_tag="PLR72_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35929.1"
                     /translation="MIVYILLSFRYRYEYYESMGELFRVMNAECLQQTLDERLWGLFL
                     ELLCEICEALEKDIDELFELIMNCSKTAELVSRMLVPSLQEIV"
     gene            8039..8509
                     /locus_tag="PLR72_01560"
     CDS             8039..8509
                     /locus_tag="PLR72_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HOK35930.1"
                     /translation="MKNKNNRLKMISEILRTHVVGSQEELLNLLKEQHCEVTQATLSR
                     DLKQLKVAKTPLSNGGYKYVLPTYNRPLPSVSENSLENSPANGAILSLEFSGQLGVMK
                     TKPGYANAIAWDIDNRSSDYILGTIAGDDTILIIPREGVSREDITEIIKEIFSN"
     gene            8564..9148
                     /locus_tag="PLR72_01565"
     CDS             8564..9148
                     /locus_tag="PLR72_01565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK35931.1"
                     /translation="MEKDFNIDEITVQVADERFLGYVDTILKTIEDAAKVRGTGIARR
                     SPEYIAQKIKEGKAIIALQGEEFAGFCYIETWSDKKFVANSGLIVVEKFRGHGLAKRI
                     KQKAFELSRQRYPDAKIFGLTTGLAVMKINSELGYKPVTFSELTSDEAFWKGCESCVN
                     YDILQRTNRKYCLCTAMLYDPAKEENKNNQPTEK"
     gene            9205..10401
                     /gene="argG"
                     /locus_tag="PLR72_01570"
     CDS             9205..10401
                     /gene="argG"
                     /locus_tag="PLR72_01570"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOK35932.1"
                     /translation="MKEKVVLAFSGGLDTSFCVKYLSEEKGFDVYTAIANTGGFSEED
                     LKKIEEKAYQLGAVKHVTLDITREFYEKGIKYMVFGNVLRNGTYPISVSSERIFQAIA
                     IINYAKEIGANYVAHGSTGAGNDQVRFDLTFQVLAPEINIITPIRDLSLTREYEIDYL
                     KQHGYEADFKKMEYSINKGLWGTSIGGKETLKSEQTLPEEAYPSQVTEKEEKIISIEF
                     DKGEICGINGEKKTDKIALIQQLENIASKYGIGRDMHIGDTIIGIKGRVAFEAAAPIL
                     IINAHKMLEKHTLTKWQQYWKDQLGTWYGMFLHEALYLEPVMRDIEKFLESSQKNVSG
                     TVYIKLRPYCFTLVGVDSPFDLMKTELGEYGETNKGWTAEEVKGFIKMMANPLKIYYN
                     IQKKNQ"
     gene            11107..12453
                     /gene="gldE"
                     /locus_tag="PLR72_01575"
     CDS             11107..12453
                     /gene="gldE"
                     /locus_tag="PLR72_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOK35933.1"
                     /translation="MFENFSNILLSVAVYPLTFSAVFSLLVIIGLLICSALISGSEVA
                     FFSLSPQIKNEFEESNKKTEKRVLELINHPQRLLATILIGNNFVNVAIILLLFYFTNS
                     IFDFSNAPVLGFIIETIVITFILLFFGEALPKIYGAQYPKKMTLFTASFISALEKIFR
                     PVASLLINSTSMVNKKLEKLKKSEISIDELSQALELTTDNKDEDKEMLEGVIKFGNTR
                     VADIMTSRIDMVAIDIKTNFKELLDLVVSTGYSRIPVYSGNRDNIKGILYIKDLLPHL
                     DKPAHFRWQSLIRQAYYVPETKKIDDLLNEFQKNKVHFALVVDEYGGISGMVTLEDIL
                     EEIIGEISDEYDEEEKLYTKIDNHTFIFEGRISLNDFFKVTGIDEEEFSKVIKEVETL
                     AGLILELKGEMPAKNEQIEYERYIFEILSIENRRIKKVKLHIKAAQDNLEENEKSV"
     gene            12549..13106
                     /gene="gldD"
                     /locus_tag="PLR72_01580"
     CDS             12549..13106
                     /gene="gldD"
                     /locus_tag="PLR72_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOK35934.1"
                     /translation="MGGVLVLFISCNRKQTPRPYGYFRVDLPSISYRMLDTLGFPYRF
                     EISSHANVAIKKPKQVDEKYWIDINYPDLNATIYCSYKSIHGNFNSLSEDSRKIVYKH
                     TVRADAIQEKVYENPSRKVYGILYELKGNTASPVQFVLTDSTRHFFRGALYFNNVPNK
                     DSIAPMLDFVRKDIIKLMESFEWKP"
     gene            13103..>13408
                     /locus_tag="PLR72_01585"
     CDS             13103..>13408
                     /locus_tag="PLR72_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35935.1"
                     /translation="MMHKYFKTREGVILIWQVTESKEELFQQLDGQTIDLLEYEKLKN
                     ERRQVEYLGTRIALKNLLNKKIDFFHTSEGKPYLEDESYQISISHSQQWIAVIAHPNL
                     "
     assembly_gap    13409..13508
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13509..14072
                     /locus_tag="PLR72_01590"
     CDS             <13509..14072
                     /locus_tag="PLR72_01590"
                     /EC_number="2.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerol geranylgeranyltransferase"
                     /protein_id="HOK35936.1"
                     /translation="LKKEIEVPIVLFPGNASQFSQEADALLYLSLVSGRNAEYLIGQH
                     VISSFAIKQSGIEVISTAYLLIDGGKVSAVEYMSNTRSIPREKKEIVVSTALAGELLG
                     MQMIYLEAGSGAIFPVPKELISYVKLFASIPIIVGGGIQTIEKLKECFDAGADIVVVG
                     NLFEIYPNKIQDFVHFTHQYNEKTTLV"
     gene            complement(14064..>14897)
                     /locus_tag="PLR72_01595"
     CDS             complement(14064..>14897)
                     /locus_tag="PLR72_01595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK35937.1"
                     /translation="IDIMPASRRGEGIGYFGIANNLAMSIGPMISLLLHDHASYRFIF
                     SISIVSALLGFISATTVKSKKEVERSANQPIVLDRFFLLKGTHAGICLTLMAIPYGML
                     TTYVAIYGKETGFQGGMGMFFTLLAIGLIFSRFFSGKMVDKGFIVKIIKIGTLICIVS
                     VLILSSLNAFYGIYPSLAEGSFYAIAVFLGVGYGMLFPAYNTLFVNLAPNNRRATASS
                     TFLTTWDVGIGLGLIIGGKIGDTQGGLSLSYFTGGIIAAISFVYFLKIAGPHFEKNKL
                     Y"
     assembly_gap    14898..14997
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14998..15479)
                     /locus_tag="PLR72_01600"
     CDS             complement(<14998..15479)
                     /locus_tag="PLR72_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOK35938.1"
                     /translation="MIINERDTRRERLLEVANEMMTAARTAPKAKGYDIIEVAVITDE
                     TIQQLSDAMLEYSQKSGLKFITRDAENILHAQAIVLIGTKQKVQNLNCGYCGFSTCNE
                     KDKFPEIPCAINTVDVGIAIGSACSVAADRRVDTRVMFSVGRVAQEMELLPGCTSIYG
                     "
     gene            complement(15505..16308)
                     /locus_tag="PLR72_01605"
     CDS             complement(15505..16308)
                     /locus_tag="PLR72_01605"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOK35939.1"
                     /translation="MKKSYEDLCLKVCKIAKETGKFIAGERISFDESKVELKGVHDLV
                     SYVDKTSEKYITEQLSKLLPEAGFIVEEGTIDQKGERFNWIVDPLDGTTNFVQGVPVY
                     AVSIALMDNNELVVGVVYEIGRNECFYTWKGGGSFLNDKPIHVSKRNRIQDALLATGF
                     PYSAFDRLDEYMKFLKWTFIETRGVRRLGSAATDLAYVACGRFDAFWEYNLKPWDMAA
                     GALLIQEAGGTISDFKGGNNYLFGKEAVATNGLLDKIMLPKLQELMQIE"
     gene            16904..18862
                     /gene="gyrB"
                     /locus_tag="PLR72_01610"
     CDS             16904..18862
                     /gene="gyrB"
                     /locus_tag="PLR72_01610"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOK35940.1"
                     /translation="MINNEKLDAINEYGASSIQVLEGLEAVRKRPAMYIGDTSLKGLH
                     HLVYEVVDNSIDEAMAGHCDNIQVIINEDNSITVIDNGRGIPVDYHEKEGKSALEVVM
                     TVLHAGGKFDKGTYKVSGGLHGVGVSCVNALSSFMRVEVARNGKLYVQEYECGIPKFS
                     VKEIGESDITGTTVTFLPDKSIFTETVFKYDIIADRLRELAFLNAGIKLTLVDKRTIE
                     EDGNYKTEVFYSKEGLKEFVKYIDEAREKLIEDIIYINTEKNGIPVEIAITYNTSYNE
                     NIHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEDSKMLEKQKIEISGDDFREGLTAV
                     ISIKVAEPQFEGQTKTKLGNNEVMGAVDIAISEALGNYLEENPKSARIIVEKVILAAT
                     ARHAARKARELVQRKSPLSGGGLPGKLADCVEKDPAKCELFLVEGDSAGGTAKQGRSR
                     QFQAILPLRGKILNVEKAMQHKVLESEEIRNIYTALGVTIGTEEDSKALNLSKLRYHK
                     IIIMTDADVDGSHIATLIMTFFFRHMKEIIEQGYLYIATPPLYLCRKGKIEEYCWTEQ
                     QRQAFIEKYGNGQDGSVHVQRYKGLGEMNETQLWETTMNPEKRTLRQVTIENAASADH
                     VFSMLMGDDVLPRRQFIEEHAIYANIDA"
BASE COUNT         6339 a         2963 c         3165 g         6327 t
ORIGIN      
        1 gttaaagttt tctgaccatt ttttagttag aaagtttttt atctaaattt tctttcaaag
       61 cagagagaaa ttcttccgta gataaatagt cttgtcgcgt gattttatca ccataaacca
      121 ataatgctaa gtctttagtc attttgcttg attctactgt ctcaatacaa acctgttcca
      181 gttcatgaca aaaatcaatt aattcttgat tgccatctag tttgcctcga tgtgataagc
      241 cacgtgtcca tgcaaaaatg gaagcgattg gattggtgga agtttctttt ccttgctggt
      301 aaagcctgaa atgtcgcgta actgtgccgt gagctgcttc agcttccata gttttaccat
      361 cgggtgttag caaataagaa gacattaatc ccaatgaacc aaatccttga gcaatggcat
      421 cagactgaac atctccatca taatttttac aagcccaaat aaaatcgcct ttccatttta
      481 acaccgacgc caccatgtca tcaatcaatc gatgttcgta agtgatacca atttcctgaa
      541 atttatcttt aaattcagct tcatatactt cttgaaaaat atccttaaac cgtccatcat
      601 aagcttttaa aatggtattt tttgtagaaa agtaaagagg taattttttt tcaagagccg
      661 cttgaaaaca agcacgagca aatccataaa tagattcatc cgtgttatac atggttagtg
      721 ctacgccatc gccattaaaa ttataaactt cccattcttg cgattcacca ttttcattgg
      781 taaaagttac ctttaatttt cctttgcctt tggtaacaaa atcggtagct ttatactgat
      841 cgccaaaagc atgacgccca atgataatcg gtttttccca gtttggaacc aaacgaggaa
      901 cattggaaca aataataggt tcacggaaaa ccgttccacc taaaatattc cgaatcgttc
      961 catttggtga tttccacatt ttctttaatc caaactcttt tattcgtgct tcgtcgggcg
     1021 ttatggtagc acatttaata cccacattat atttttttat agcataagct gcatcaatag
     1081 ttacctgatc gtccgtagca tcacgatttt caatgcttaa atcataatat tgaatattta
     1141 aatcgagata aggaagaatg agctgttctt taatcatttt ccacattact ctggccattt
     1201 catcgccatc taattcaaca ataggttgat ttacttttat cttgttcatt cgttttgtat
     1261 tattataaga atttgttcct tatcttttac ttaaaggaat atattcttga ttttcattga
     1321 catttttgta agccggacga ataatacgtt tactttcaaa attaagttcc tcaattctgt
     1381 gagcacacca gccaactacg cgtgccattg caaagatagg tgtataaact tcttgtggca
     1441 aaccaatggc ttgataaaca aaacctgaat aaaagtcaac attaatgcaa acttgtttat
     1501 ttacatcttt tcctttatat tccataaatg ttttgatggc gcgttcttca atcagttcca
     1561 gaaaattaaa ttctttttca agtccttttt cctttgctaa ttcacgtgcc atttttttca
     1621 ataatactgc tcttggatcg ctaatggtat aaactgcatg tcctactccg tagattaagc
     1681 ccgatttgtc gtaagcttgt ttgttcaaaa ttttgagcaa atatttatct atttcttcag
     1741 gatttgtcca gtcagcaata ttttttttca tgtcgtcaat catgcccatt acttttaaat
     1801 tagcgcctcc atgaagtgga cctttcaaag aaccaattcc tgcagcaata gaagaatagg
     1861 tatcagtttc ggtagaactg gttacgcgta cactgaaagt agaattattt ccaccaccat
     1921 gttcagcatg caaaataagt gctaaatcga gaatttgtac ttcaagagga gtgtattgat
     1981 cttttccctt tatcatataa agaaaattct cggcaaatga aaaattttcg tttggatgac
     2041 gcacgtgtaa ggaacgtcct ttttcatgat gacgcaatac attataggca taagaaataa
     2101 tagttggaaa cttggcaatc agattaagag actgtttgat aagattgtct cgtgaaatat
     2161 tatccggatt ttcatcataa atataaagtt ccaatacgct acgagctaaa atattcatta
     2221 tgttatgtcc tttaaggttc aaaatactaa tggtagcttg atgttccaac ggcatttttt
     2281 cataaataat atgtgaaaaa gtttccaatt cttcgttgtt aggtaaatat cctgaaagaa
     2341 gtaaataagc agtttcctca aatcccaatc gattttcttt ctgaatgcca tttactaact
     2401 cttccacatc aattccacga taataaagtt taccaggaat gggtttcaat tcaccagttt
     2461 ctgttcgttt atagcccact acgtctccta tacgcgttaa gcctaccagc acaccagtat
     2521 gatcttcatt tcttaatcct cgtttgacat tatattttgt aaaaagttct ttgtcgattt
     2581 tactggtctc cttaatggtt tcggagagtt tataaataag aaattcttct ttcattgctt
     2641 tttttaattt ttgaaaatga atatatgttt gttttatatt tttgtctctg aattttaatt
     2701 gtttcctttt tcaaattgtt cttttatgaa atttaaagca ctgcctgcct taaaccactc
     2761 aatttgagtt tgattataac tatgtgcaac ctcaaattgt tcagtagtgc catcactgtg
     2821 atgcaactga atttttaaat ttttaccagg agaaaattcg gttaatccta agatactgat
     2881 tttatcatct tccctaattc tatcataatc tgatttgtta atgaaggtaa gagcaagcat
     2941 accttgtttt tttaaattgg tttcgtgaat gcgagcaaac gatttagcta taatagcttt
     3001 tacattcaaa tagcgaggct ccattgctgc atgttcgcgt gatgatcctt ctccataatt
     3061 ttcttcggct actaccacac ttccaatgtt ttctgcttta tatcttcttg ctgctgcaga
     3121 aacaggcatg tattcaccgg taggaggata aagaacgcta tttgtcttat tgttgaaagc
     3181 atttacggct cccattaata gattatccga aattttgtcc agatgaccgc gataacgcaa
     3241 ccagggacct gccattgaaa tatggtctgt ggtgcattta cctttgactt taattaacaa
     3301 agccaattct atcatatctt ttccatccca ggctgggaaa ggctgtaaca gttgcaacct
     3361 ttcagaatgt gggtcaatat tgataggcaa tttttcccca gtaggagcaa tataaccttt
     3421 ttcttttgtt tcatagccaa ctaaaggaag ttcaaaacca atcggttcgc ttaactttac
     3481 tttttctcct ttttcattta ctaagaaatc aatcaacgga ttaaagcata aatcaccggc
     3541 aatagttaat gcggcaacta tttcaggaga agcaagaaaa gcatgggtgt tgggattacc
     3601 atcgtttcgt ttggcaaaat ttctattgaa agtagtaaca atagaattag gacgatcagc
     3661 aggaatcaat ttgcggttcc attgaccaat gcaaggaccg caagcatttg ccataataac
     3721 tccacctact tttgtaaaaa cttccaatat accatcacgt ttagcagtag ttctgatttg
     3781 ttctgaacca ggattgatta aaagttctgc attaattttc aatttttttt caatggcttg
     3841 ctcaaaaaca gaagcagctc ttgataaatc ttcataggaa gaattggtac aagaaccaat
     3901 taatccaact tccatttttt gcggataatt tttttctttc actattttgg caaattttga
     3961 aatagtataa gctgcatcag gtgtaaatgg gccattgata tgaggttcta tttcatttaa
     4021 attaatttca attactttat cataaaaatc ttctggatgt gctaacactt cagaatcggc
     4081 agttaaataa ttactcaatt cgcttgctat tttagcaatt gagtttcgac cggtagcatt
     4141 gagatattgt tcagattttt tatcgaaagg gaatatggaa gtagtcgctc ctacttcagc
     4201 ccccatattg caaatagtgg ctttccctgt tgcagataat gtttcggtac cttcgccaaa
     4261 atattccaaa acagcatttg tagcaccttt tacagtaagt attccggcta attttaaaat
     4321 tacatctttt ggtgatgccc aacctgaaag ttttcctgaa agttttactc caatgatttt
     4381 tggcattctt aattcccatg taagtcctgc cataacatct actgcatcag cacctcctac
     4441 gccaatagct atcatgctca atcctccagc agttggcgta tgcgaatcag ttcctatcat
     4501 cattccacct ggaaaagcat aattttcaaa gattatttga tggataattc ctgctccggg
     4561 ttcccagaaa taaattccat aattattaga aactactttc aaaaaatcat atatttcctg
     4621 attaatttct ttggcttttt ttaaatcttc aactgctgaa atatttgctt caattaaatg
     4681 atcgcaatga acagaagtag gtacagcaac tttttctcgt cctgaattca ttaattgtaa
     4741 aagtgccatt tgagcagtag catcctgcat agctactcta tcaggaataa aattgacata
     4801 atctattcct cgtcgaaagt tttttatcgt ttcattaggt gctaaatggg tatataaaat
     4861 tttttcagta agtgttaatg gatgtctaag ctttgattgt attttttcaa tttttataga
     4921 aaatgttgca taaaattgct gaaaaatttc ttcgtttgaa atcatatttt tcttattttt
     4981 attcatctaa taacaatttg ttttgcaaaa gtaataaaaa agttacaatc actctatttt
     5041 atttaaagca attattcatt ataataaaat ttaattttat ttattttctt gaataaatat
     5101 acagttgatt ttatcattaa gttatcaaga aagtatttaa ttgaaacaaa aatatttgct
     5161 aataaatact tgacattttg atttttattg agagactcta aatatttgtg cagtaaattt
     5221 tttaagtttt atgtaaatta aataattata gttcaagttt ccttttaacg atgaaaaaga
     5281 tttttaaaac tattcattta tttttgatta caaaaaaatg cttacctttg aacaaaatta
     5341 aaaatctttt accctataga ttaatttttt atgaaaagac aattaaaaat agctcttgta
     5401 gctcatgata atcgaaaagt tgacatggta gaatgggctt tatataatgc tgaaatgtta
     5461 tctcagcaca aattattttg tacaggtact acaggtaaat taataaaaaa agcaatggca
     5521 gaaagaggaa ttgaagttga cattacttgt atgcattcag gacctttggg aggtgatgct
     5581 gaaatagctg ctcttgttgt tcagaaaaaa attgatttgg cagttttttt aatcgatgat
     5641 ctcaatcctc aaccacacga agcagatatt caaatgttgc ttcgtcaatg tagaattcat
     5701 aatgtaccac tggcttgtaa tcgttacagt gctgatttaa tgattaccag cactttgtgg
     5761 gacgatgaaa cttatgtgcc tacagaaccg aaatatgtta aatttgtgcg agaagatttt
     5821 tttaattctg atgaagattt agattaaaat aaaaataata gaataactaa agtttcgcat
     5881 ttcagaaagt gctgggaata aagtaaaaaa gaagataaaa aagtaaaaag tagtatatat
     5941 taaaagtagt atatattaac tgtatagtta aaaaaataaa aatatgctac cggacaaagt
     6001 tagaagaagg attttgtgag attgaaacag atttttatga aaataaatta gagagtataa
     6061 atattttgaa aggtttcaag gcgttgaaac tgacggaaag tttttcaaag ttcaagaatt
     6121 tgaaaaggag tagttacagt tttcaagtga tattgttggc gttgatttaa tcatgtcaca
     6181 aggtggaaaa cgataggatc ttccctgtct tgttaaaaga aggagttata acgataaata
     6241 aggatgtttt gtttcgtttg aagaataatc aacgaactca ttcacgaaag atattatgga
     6301 aacctatcag attagatgct taatagaaac acttttcaaa gagacgaagc aattgttgaa
     6361 tctggaagga tggcaatcca atgatttcga tgcacaaaat agccgatacc attgtaagta
     6421 ttgatagtct acattttgtt atcttttcga tatcgatatg agtactatga atcgatggga
     6481 gaacttttca gggtaatgaa tgcagaatgt ttgcagcaga cgcttgacga acgtctttgg
     6541 ggattatttt tggagttgct gtgtgaaata tgcgaagcat tagagaagga tattgatgaa
     6601 ttgttcgagt tgatcatgaa ttgttctaag acggcagaat tagtttcaag aatgcttgtt
     6661 ccttcactcc aggaaatagt atgacgggaa tggaattatt aacatattga taatcaggat
     6721 aaaaataatt aaatcgtact tcattgtttt tatttgtcaa accattaaat caaagtacga
     6781 aaaagcatta gtaaaataaa aaattattct ttacttatat tcaataagga aaaatccact
     6841 cttccccaga accatttttg gtttttaacg taaaaatttt cattgttttt atcatttttt
     6901 gtctttaaaa cttcatcaaa aacagacaat tttcataacg cttaagtttt acctcactca
     6961 taaccatatt accttattta cttgggttga ttattttctt tctttccatc tgtattttat
     7021 tctgctcttt ataaggtaat aaggtttgat ttatttgttt gttaatggtt atgaaggttt
     7081 ttaaaatgat aaggtttcaa ttttaaaact tgttttttcc atattccata ttgaaaaatc
     7141 tattttttcc catgttcaaa ttaataattt tattgtcgga cgaataataa aatgcatttc
     7201 gaaaaggcag cgtcttttta ttaaactttt tgtccacgac aaaaataaac gttgccgtat
     7261 catattggta ttcagtaatt aaaaagttat tttggattga ctttgttcgg cgagccgaaa
     7321 attgacgagt tttggatttg cgaatttcga gtatttatag tttcgtttaa caacacaacg
     7381 ataaaagaat catcttttgt tcatcagtat aataagtcat ttattttcat agaattccac
     7441 agagaaggta caaagttata gagagaaaaa tctcaaaaaa caaacgaaat agtaaaacgt
     7501 tttttacttt tttcaaaaca attgaagcga agctgaaatc actgaaacaa ctaaaaaagt
     7561 gaaacatttt ttaagaaaaa ataaagtgag atttggagca aagtccctca tataaatgcc
     7621 tgattgtcag aaaattttag acagttaaga atttttgtca tgtacttaga aattttgaag
     7681 caaagaattt aattcctcat tgatgttcct gagggtaagt atttttatta gtatttatgt
     7741 ttttgaaata tcataatggt tttataaata ttcattttta atttattttt taggaagatt
     7801 tgattaaata attataaaaa ttaattttta ttttttagta catgttataa aagtcataat
     7861 aatttttaag gaaagaatat ttataattat ttaatatcat ttttaagaat aaatatagta
     7921 attttgcaaa gattttttaa taaatattca ttacgacatg aattatatta aaaaattata
     7981 cacaaaaagt tttatgggca aattcctatg atttttgata taaaatagac aacaacttat
     8041 gaaaaacaag aataaccgtt taaaaatgat ttctgaaatc cttcgtacac atgttgtagg
     8101 aagtcaggaa gaattactaa atcttttaaa agaacagcac tgcgaggtaa ctcaagcaac
     8161 tttatcgaga gatttaaaac aattaaaagt tgccaaaact cctctctcaa acggtggata
     8221 taagtacgta ttacccacct ataatagacc tttgccttcc gtttcagaaa attctttgga
     8281 aaattctcca gctaatggag ctattttaag tcttgaattt tcaggacaat taggcgtaat
     8341 gaagacaaag cctggttatg caaatgccat tgcgtgggat attgacaatc gttcttctga
     8401 ttatatttta ggtaccattg caggcgatga tactatttta attattccac gtgaaggagt
     8461 atccagagaa gacataacag aaattataaa ggaaatattt agtaactgat aatcaaattt
     8521 taagtaaatc tttaaaaagt attttaaaat agatttaaaa aagatggaga aagattttaa
     8581 tattgatgaa attacggttc aagtagctga tgaacgtttt ttaggatatg ttgatacgat
     8641 tctgaaaaca attgaagatg ctgctaaagt gcgtggtaca ggcatagctc gccgttcgcc
     8701 agaatatatt gcacaaaaaa taaaagaagg gaaagctatt attgctcttc agggagagga
     8761 atttgcagga ttttgttata ttgaaacgtg gagcgataaa aaatttgtag ccaattcagg
     8821 attgattgtg gtggaaaaat ttcgtggtca tggattagca aaacgaatca aacagaaggc
     8881 tttcgaactt tcacgtcagc gttatcctga tgcaaaaatt tttggattga ctaccggatt
     8941 ggctgtaatg aaaataaatt ctgaattggg ctataaacct gtaacatttt cagaacttac
     9001 cagtgacgaa gctttttgga aaggatgtga aagttgtgta aattatgata ttctccaaag
     9061 aactaatcgc aaatattgcc tttgtacagc tatgctttac gatcctgcaa aagaagagaa
     9121 caaaaataat cagccgactg aaaaatgatt tatcagttgt tatttatcga taaaaaaata
     9181 attttaaact aattataact taaaatgaaa gaaaaagttg ttttagcatt cagtggaggt
     9241 ttggatactt cgttttgtgt aaaatattta tcagaagaaa aaggatttga tgtttacact
     9301 gctatagcca atacaggagg cttttcagaa gaagatttaa aaaaaattga agaaaaagct
     9361 tatcaattag gagcagtaaa acatgtaaca ttagacatta caagagaatt ttatgaaaaa
     9421 ggcattaaat acatggtttt cggtaatgtt cttcgcaatg gcacttatcc tatttcagtt
     9481 agctccgaaa gaatttttca agcaatagcc atcatcaact atgcgaaaga aattggtgct
     9541 aattatgtag ctcatggaag tactggagcg ggaaatgatc aggttcgttt cgatttaaca
     9601 tttcaagtgt tggctcctga aataaatatc attactccca ttcgtgattt atctctcacg
     9661 cgtgaatacg aaattgatta tttaaagcaa catggctacg aagctgattt taaaaaaatg
     9721 gaatactcca ttaataaagg tttgtgggga accagtattg ggggtaagga aacattgaaa
     9781 tcagagcaaa cgcttccaga agaagcttat ccttcgcaag taactgaaaa agaagaaaaa
     9841 attatttcta ttgaattcga taaaggagaa atttgcggta tcaatggaga aaaaaagaca
     9901 gataaaattg ccttgattca acagttggaa aacatagctt caaaatatgg aattggtaga
     9961 gatatgcaca ttggcgatac cattattggc ataaaaggac gagtggcttt tgaagctgca
    10021 gctccgatat taatcattaa cgctcataaa atgcttgaaa aacacacact taccaaatgg
    10081 caacaatact ggaaagatca attgggtacg tggtatggta tgtttctgca cgaagcactt
    10141 tatctggaac ctgttatgcg agatattgaa aaatttctgg aaagttctca gaaaaatgtt
    10201 tctggaaccg tttatatcaa acttcgacct tattgcttta ctctggtagg tgttgacagt
    10261 cctttcgact tgatgaaaac tgaacttggt gaatatggcg aaaccaataa aggttggaca
    10321 gccgaagaag taaaaggatt tatcaaaatg atggcaaatc ctttaaaaat ttattataat
    10381 attcaaaaga agaatcaata aataatataa attaaatgat tatccacatt taactcgaca
    10441 agtgaattac tttttgtaac ttttgttttt tagtatctga taaattttat attaaattga
    10501 tggttatcga ttcattctgt tgtttttgaa ttgatctcgt ccagtggact gaaaactgac
    10561 aagtttcgag ttctcgagtt ttgagtattg caaggtgtgt ggattcgagg tcgaggtgca
    10621 aatttggagt cacaagtttg gagcaacaaa agaaaaaatt tctgtgtttt ccctgtgtaa
    10681 attgtcgtgt tttttgcgta aaaaatcgtt tatttttaca aagctccata aagaaggtat
    10741 aaaaattaca ggagaaaaat tttttataag ataatttagt agttacagtg ggtgccaaga
    10801 caattgaagt gaagcgatat cgccgaaatg actaaaaaat aacacttttt gaagcaaaaa
    10861 agtaagtaga ttttgggcaa agcccaattt cgaaatagta ttccattgtt catttgtaaa
    10921 aatgacgtta tattattcca aattaaattt aatcaaaaga attcctcgag gcttgcctcg
    10981 aggttattca ttcccggtat aataaatcat attataaatt ttactcaccc agcaatctcc
    11041 aaacaaaaaa atagctttat atttgtattt taaaaaatta tattgaaata ctttttgaaa
    11101 ttaataatgt ttgaaaattt ttcaaatatt ctattaagtg tcgctgttta ccctcttact
    11161 ttttcagccg ttttttcttt acttgtaatt atcggcttgt taatatgttc tgctcttatt
    11221 tcaggatcag aagtagcttt tttctcctta tctccacaaa ttaaaaatga atttgaagaa
    11281 agtaataaaa aaaccgaaaa aagagtttta gaacttatca atcatcccca acgcttgctg
    11341 gcaactatcc taatagggaa caatttcgtt aatgttgcta tcattttgtt gttgttttat
    11401 tttaccaatt ctatttttga tttttccaat gctcctgtgt tgggttttat tatcgaaacc
    11461 attgtaatta cttttatttt gctgtttttt ggagaagctt tacctaaaat ttatggagca
    11521 cagtatccta aaaaaatgac actttttaca gcttctttca tttcagcact ggaaaaaatt
    11581 ttccgtccag tagccagttt attgattaat tcaacttcta tggttaacaa aaaacttgaa
    11641 aaactgaaaa agtcagagat ttctattgat gaactttcac aggcattaga attaacaact
    11701 gacaataaag atgaagataa ggagatgctt gaaggtgtta ttaaatttgg caacactcgt
    11761 gtagctgata ttatgacttc acgtattgac atggtagcaa ttgatattaa aaccaatttt
    11821 aaggaacttt tagaccttgt tgtttctaca ggatattctc gtattccagt ttattctggc
    11881 aatcgcgata atatcaaagg tattctttat atcaaagatt tgttgcctca tctggataaa
    11941 cctgcccatt ttcgctggca aagccttatc aggcaagcat attatgttcc tgaaacaaaa
    12001 aagattgatg atttgctgaa tgaatttcag aaaaacaaag ttcattttgc actcgtggtt
    12061 gacgaatatg gtggaatttc aggaatggtt acactggaag atattctgga agaaattatt
    12121 ggtgaaatca gtgatgaata cgatgaagaa gaaaaattat atactaaaat cgacaatcat
    12181 acttttattt ttgaaggaag aatatcgttg aatgactttt tcaaagtgac aggaattgat
    12241 gaagaagaat tctcgaaagt aataaaagaa gtagaaacac tggctggcct cattctggaa
    12301 ctgaaaggag aaatgcctgc caaaaatgaa cagattgaat atgaacgtta tatttttgaa
    12361 attttatcga tagaaaacag acgaataaaa aaagtaaaac ttcatatcaa ggcagcacag
    12421 gataatttag aagaaaatga aaaatcagtt tgaaatgatt ttaaccttcg tgtttcccat
    12481 attaaatatt agatttgaaa gtaaatgttg gataaccaat gaataaacat ctcttaaaaa
    12541 cattgacgat gggtggagta cttgtactgt tcatttcctg taacagaaaa caaacacctc
    12601 gcccgtatgg atattttcgc gtagatttac cttcaatttc ttacagaatg ctggacacgt
    12661 taggttttcc ctatcggttt gaaatttcat cccatgccaa tgttgctatt aaaaagccaa
    12721 aacaagttga tgaaaaatat tggatagata ttaattaccc agacttgaat gccactattt
    12781 attgtagcta caaatctatt catggaaatt tcaatagttt gtcagaagat tcacgtaaaa
    12841 tcgtttataa acatacagtt cgtgctgatg ccattcagga aaaagtttat gaaaatccat
    12901 ccagaaaagt atatggaatt ctttacgagt taaaaggaaa tactgcttca cctgtgcaat
    12961 ttgttctgac agacagcaca cgacattttt ttagaggagc cttgtatttt aacaatgttc
    13021 ccaataaaga ttcgatagct ccgatgctag attttgtacg aaaagatatt atcaaattga
    13081 tggaatcttt tgaatggaag ccatgatgca taaatatttt aagacgcgag aaggggttat
    13141 tttgatttgg caagtgacag aaagcaaaga agaacttttt caacaattgg atgggcaaac
    13201 aattgattta ttggaatatg aaaaactgaa aaatgaaagg cgtcaagtag aatatttagg
    13261 aactcgaatt gctttaaaaa atttgctgaa taagaaaatc gatttttttc atacttctga
    13321 aggtaaacct tatctggaag atgaaagtta tcaaattagt atttctcata gtcagcaatg
    13381 gatagctgtg atagcccatc caaatcttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13501 nnnnnnnnct gaaaaaagaa attgaagtgc caattgttct ttttcccggc aatgcttctc
    13561 aattttcgca agaagcggat gccttattat atctttcctt ggtttcgggt agaaatgcgg
    13621 aatatttgat tggtcagcat gttatttcct cttttgctat aaaacaatca ggaattgaag
    13681 taatttcaac agcttattta ttaatagatg gtggaaaagt aagtgcagtg gaatacatga
    13741 gcaacactcg ctccattcca cgtgaaaaaa aggaaattgt agtttcaact gctttagctg
    13801 gagaattatt gggaatgcag atgatttatc ttgaagcagg aagtggagca attttcccgg
    13861 ttcccaaaga attaatttct tatgtaaaat tatttgcttc tattcctatt attgtaggtg
    13921 gtggtattca aacaatagag aaactaaaag aatgttttga tgcgggagca gatatagtag
    13981 tagtaggaaa tttatttgaa atctatccca ataaaattca agattttgtc cattttactc
    14041 atcaatataa cgagaaaacc acattagtat aatttgtttt tttcaaaatg aggtcccgca
    14101 attttcaaga aataaacaaa tgaaattgct gctataattc cacctgtaaa atacgataag
    14161 gacaatccac cttgtgtatc accaattttt ccaccaatga taagtcccaa gccaattccg
    14221 acatcccatg tggtcaaaaa agttgagctg gcagtagctc ttctgttgtt gggggcgaga
    14281 ttgacaaaca aagtgttata agctgggaac aacattccat aaccgacacc taagaagact
    14341 gcaattgcgt agaatgatcc ttcagcaagt gaaggataaa tcccataaaa tgcatttaac
    14401 gaagacaaaa taagaactga aacgatacaa attaatgtac caatttttat tattttcact
    14461 ataaaacctt tatcaaccat tttccccgaa aagaaacgtg aaaaaatcaa accaattgcc
    14521 agcaatgtga aaaacattcc catacctccc tgaaaacctg tttcctttcc ataaattgcc
    14581 acataagtgg ttaacattcc ataaggtatt gccattaaag taagacaaat cccagcatgt
    14641 gttcctttta ggaggaaaaa acggtctaat acaattggtt gattggctga acgctcaact
    14701 tccttttttg acttaacagt ggttgcggag ataaagccca acaaagcact tacaatggaa
    14761 atggagaaaa taaaacgata agaagcatga tcatgtaata aaagactaat catcggtccg
    14821 atggacattg ctaaattatt agcaatacca aaatagccaa ttccttcacc tcttcgagaa
    14881 gctggcataa tatcgatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatt
    15001 ccataaatag aagtgcaacc cggcaaaagt tccatttctt gagctacacg tcctacagaa
    15061 aacattacac gagtatctac tcttctatcg gcagcaactg agcaagctga cccaatagct
    15121 atccctacgt caactgtatt tatagcacac ggaatttcgg gaaatttatc tttttcgtta
    15181 caagtggaga aaccacaata gccacaattc aaattctgaa ctttttgttt tgttccgata
    15241 aggacaatag cttgagcatg aaggatgttc tctgcatcac gtgtaataaa tttcaaacca
    15301 cttttttggc tatattccag catggcgtct gataattgtt ggatagtttc atcggtaatc
    15361 accgctactt ctatgatatc atatcccttt gctttgggag cagttctggc ggctgtcatc
    15421 atttcatttg ccacttcaag taaacgttcc cgtcgagtat cacgctcatt aattatcata
    15481 attcaatttt tttgacattt atttttattc aatttgcatt aattcttgca attttggcaa
    15541 cataatcttg tcaagcagac catttgttgc tactgcttct tttccaaaaa gataattatt
    15601 gccacctttg aaatcggaga ttgttcctcc ggcttcttgg ataagtaaag cacctgcagc
    15661 catatcccat ggcttaagat tgtattccca gaatgcatcg aagcgtccac aggctacgta
    15721 agccaaatcg gtagcagccg atcccagccg acgaacacct cttgtctcaa taaatgtcca
    15781 ttttaaaaat ttcatatatt cgtccaatcg atcaaaagca gaataaggaa agcctgtggc
    15841 aagtaaagca tcttgtatgc gattacgttt tgacacatga atgggcttat cgtttaaaaa
    15901 tgaacccccg cctttccatg tataaaaaca ttcattacgt ccgatttcat atactacgcc
    15961 tacgaccagt tcattattat ccatcaaagc aatgcttacc gcataaactg gcacgccctg
    16021 tacaaaatta gtagtgccat caagtggatc gactatccag ttaaaacgtt cacctttttg
    16081 gtcgatagta ccttcctcga caataaaacc tgcttcgggt agtaatttac ttaattgttc
    16141 agtaatatat ttttcagaag ttttatctac atagcttacc aaatcgtgca cgccttttaa
    16201 ttcaactttg ctttcatcaa acgatatgcg ttcccctgca ataaattttc ctgtttcttt
    16261 tgctatttta caaactttta gacaaagatc ttcgtaactt tttttcatca ttcattatcc
    16321 tttattaaaa tttttgcaaa attagggttt tctaaaaaaa taagcaagac acatttttta
    16381 attattttgt cgattagaga aattaatttt tgagatattc aaaaataagt taataaggtt
    16441 tgattgtttt gtttgtcata agttattaag gttttaaata caatagggtt tgtttaactt
    16501 gctccttact tgttcaatag cgaaaaatct acttttttgt taaacttttt aggcaaaaaa
    16561 tttaatgttt ctataataag tatttttata tctatctgta tttttaaata ttccgtagca
    16621 ttttttttca caccatagat ttgtaagaaa aagagcatca aattttctgg aaaaattttt
    16681 ttgttacacc gaatttgaaa tttcaatttt cttatcatta ttattaataa aatttttata
    16741 tatttttctg attttcaatg aatttattta ttcattaaag gtcatttttc cgacaaaaag
    16801 ttaggtcatt tcaaattttt ttcttaaatt tgtatgattt ttaaaaggac taaaaagttg
    16861 aaatttttat tcaaaattaa atcaatttaa actcaaaata aaaatgataa acaacgaaaa
    16921 attagatgcc atcaatgaat atggtgcgag tagcattcaa gttttagaag gattagaagc
    16981 tgtgcgtaag cgacctgcca tgtacatagg cgatacatca cttaaaggtt tacatcattt
    17041 agtatatgaa gtagtagata attctattga cgaagcaatg gctggtcact gtgataatat
    17101 tcaggtgatt attaatgaag ataattctat cacagttatt gataatgggc gtggtattcc
    17161 ggtggattat catgaaaaag aagggaaatc agctcttgaa gtagtaatga cagttcttca
    17221 tgctggtggt aaatttgata agggtactta caaagtttca ggtggattgc atggagtagg
    17281 cgtttcttgc gttaatgccc tttcttcttt tatgcgtgtg gaagtcgctc gcaatggaaa
    17341 actttatgtg caggaatacg aatgtggtat tccaaaattt tcagtaaaag aaataggaga
    17401 atcagatatc acaggcacga ctgttacttt cctgcctgac aaaagtatat ttactgaaac
    17461 tgtattcaaa tatgacatta ttgccgatcg gttaagagaa ttagcttttc tgaatgctgg
    17521 tattaaatta acgttggtgg ataaacgtac gatagaagaa gatgggaact acaaaacaga
    17581 agttttctat tcaaaagaag gattgaaaga atttgtcaaa tatattgatg aggctcgtga
    17641 aaaacttatc gaagatatta tatatatcaa tactgaaaaa aatggaattc cagtagaaat
    17701 tgccataacc tataatacct cttacaatga aaatattcat tcctatgtta ataatatcaa
    17761 tactattgaa ggaggaactc atttggcagg atttcgtcgt ggtttaacgc gtaccttgaa
    17821 aaaatatgct gaagacagca agatgttaga aaaacaaaaa atagaaatta gcggagatga
    17881 ttttcgggaa ggcttaactg ctgtaatttc tattaaagtt gcagagccac aatttgaagg
    17941 acaaaccaaa accaaattag gtaataacga agtaatgggt gctgtagata ttgctatcag
    18001 tgaagctttg ggaaattacc tggaagaaaa tcctaaatca gcacgcataa ttgttgaaaa
    18061 ggtaatttta gccgccacgg ctcgtcacgc agcccgtaaa gctcgtgaat tggtacaacg
    18121 taaatcacct ctttcaggtg gtggattgcc cggtaaactt gccgattgtg ttgaaaaaga
    18181 tcctgcaaaa tgtgaattat ttcttgttga aggagattca gcaggtggaa ctgcaaaaca
    18241 aggtcgtagt cgtcaatttc aagctatatt gccgttgcgt gggaaaattt taaatgtaga
    18301 aaaagccatg caacacaaag tgctggaaag tgaagagata cgcaatatat atacagcttt
    18361 aggcgttact ataggaacag aagaagatag taaggcacta aatttaagta aactgcgtta
    18421 tcataaaata attattatga cagatgcaga tgtggatggt agccatattg ctacgcttat
    18481 tatgactttc tttttcagac atatgaaaga aataattgag caagggtatc tgtatattgc
    18541 tactcctccg ctttatttat gtagaaaagg gaaaattgaa gaatattgtt ggacagaaca
    18601 acaacgtcaa gcttttattg aaaaatatgg aaatggtcag gatggttctg tacacgttca
    18661 acgctataaa ggtttaggtg aaatgaatga aactcaattg tgggaaacaa caatgaatcc
    18721 tgaaaaaaga actttacgtc aagttactat tgagaatgca gcttcagccg accatgtatt
    18781 ttcaatgtta atgggagatg atgtgcttcc tcgtcgtcag tttattgaag aacatgccat
    18841 ttatgccaat attgatgctt aatttataaa aacagaatta atttattctt caacaaaaac
    18901 gaataaatga atattgccgt tttttgttcc tcgagtaatc atatcgctac ctattatcat
    18961 caagttgctt tccatttagg agaatacatt gctt
//
LOCUS       DAOSAS010000018        18897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_2035_length_18897_cov_8.597973, whole genome shotgun sequence.
ACCESSION   DAOSAS010000018 DAOSAS010000000
VERSION     DAOSAS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18897
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2035_length_18897_cov_8.597973"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..492)
                     /gene="ruvX"
                     /locus_tag="PLR72_01615"
     CDS             complement(58..492)
                     /gene="ruvX"
                     /locus_tag="PLR72_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOK35941.1"
                     /translation="MGRILALDYGRKRVGIAITDPLQISANALETVHVHEIIPFLEKF
                     LAKENVEKILIGYPLQMNGQESESMKYIKPFVASLQKKFPDIELIYVDERFTSTLAHK
                     AMLKGGLKKKDRQNKALVDKISATIILQSYLESIKNKNNISD"
     gene            1168..3900
                     /locus_tag="PLR72_01620"
     CDS             1168..3900
                     /locus_tag="PLR72_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK35942.1"
                     /translation="MKKILMTVILLTTIMLVSYSRTIYVATTGNDSNPGTETSPYLNI
                     QKAIDAAAPGDTIYIRGGTYMLTKRIKIEKAGTADARICLWGYPGERVIIDGSQIVAN
                     STNEFKQARCIYVNHFGDYWHFKNLELCNAKDNGMKLEGSYNIVEKCKFYGNNDTGLQ
                     IGMYKDFSIEETQSFPISGEPQFNPGYTYCKYNIVINCDSWYNCDTKSYSGSDDGKDA
                     DGFAAKLFPGPGTEFHGCRAWQNSDDNWDLYMVYHPILIDNCWSWKAGYDKNGTAFGN
                     GNGFKLGGGGTSGGAAFPQSVGAHVVKNCIAFDCLHKGFDQNNAYEAMYLFNNVSWNN
                     EYNYRFPTIFMYGTMYMRNNIGFKPVTLNHEFLSADKPGSQVPNTAYNSWTTIDGCDP
                     YKDGNKVNGVKVYAKDHSAQFKSLSSTLFLADRQVDGSLPENDFCKLVEGSIFINAGE
                     NIENFVPQAHSPGGLTLPPVTILYNDGRADMGAFETGEPTIATLSLISGKADQFVYVG
                     TAIINTVYKWGGAATDVIVRDLPDGLNAIKDMTAKTVTISGIPTVEGTYSIENIGGTN
                     TIVLTGNIRISNIAPATLTITQGHAIQEVFFGEPIETIVFTWGGGATDITYTALPAGL
                     TATKDIENKTLTISGVPTTDGSFTVTTLGGMEGSQVTINCNIIRVLRTKVLTGDWYHI
                     QDPYENRPADLQGNVVTFATGSNSSYPTVWDPTYVESTGSAPSGCTPGAINVERNGGS
                     VTWNLPSLVEMKSNIHFTGTRTLKIEWTIDGVTKTWTSASLSKRTMLNWDMMADIGLE
                     PTTKPVTIKFINTSTSGGIRIYDFYIKVYEDQVSGIEEVKVQTNKFNMYQTETALIVS
                     GDIAKLTVYSLSGSVVAQSEMSQVVKINHLNQGVYVVHITDKEGCQVTQKFIKK"
     gene            3984..6758
                     /locus_tag="PLR72_01625"
     CDS             3984..6758
                     /locus_tag="PLR72_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK35943.1"
                     /translation="MKNKLFFVTLIFFFFGFLLSAQNTIVENFETGLPTSAPSSETSV
                     ALSTGIWKIKGVFAKTDNGSIRLAMNTNGYAITPPIDKPVSVSFKHRGSGSGKVLTVQ
                     KSIDDGSSWTTIGTATVSSSSTYNTANISINEQGTIGVLLKFICGSSTIYIDDVTIVC
                     SSIVDEPTIQASISASEITATSMKINFNKGNGEGRLLVYSKGEEVNWLPEDGIAYTNL
                     PKVLTDNVIGVYSGNVDFVTVTGLEAGETYYFYVFEYNGANDAINYLTQPVGKLTQRT
                     VEAPSITTSVNAINFGSVKIGISSKRSFTLLAKYLTPENGTITLTSSNDFLISINSSS
                     GFGTSLTLNYSSATLLSTTIYVQFLPSELKSYSQILSISGGNAEAQVQLIGIGSNTDA
                     KVYFISPSGNDNAEGTFDAPWYNLQKAIDAALPGDTILVRGGTYYPTMMKDGTKTTIR
                     INSSGTADKRITIKNYPSEFSILNFKDQPKKQGIRGVQLNGDYWHIIGLHITQAGDNA
                     IKLEGNHNIIEKCTFSYNDDTGIQLGFGHNFSDTHPGISSNDGSYCAYNDIIDCDSYL
                     NCDADNFGSDADGFACKMHNGKGNRFIRCRSWDNADDGWDLYETDYPVILIECWAWGS
                     GRAENFDWVQASGSFQGNGNGIKMGGNGTGGSSKGKHEAWNCIAFNCNKTGSVKGFDQ
                     NSHGDGEKLVNCLAFGCGYDFMFEKSGTNCEYYNNVCFGNIEIASGCTESNNAMLSTS
                     TKAWKNNVIRGFSSSDYVSLSEDDAKAPRGADGSMPKRFARLKSGSVLVDKGLSYNVP
                     YTDEFPFLFQPIYGLARDLGPYELQEGVISGMQIIINPKAEFNLSILSNPCKSEAMIK
                     FSSDFSGIASLKIVALDGHVIEECYNKNVEAGVEYFVSISVNNLQNGMYLCVLSIGGQ
                     TKTAKLIVAK"
     gene            6856..8754
                     /locus_tag="PLR72_01630"
     CDS             6856..8754
                     /locus_tag="PLR72_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOK35944.1"
                     /translation="MKKSFLLYYVLILISVFSTNAFGRKYYISPTGSDSNDGLTLQTA
                     WGTPNFAFQTIQSGDTLWVKGGTYIVNSTVKAKNGGTKTAPVCVFAIRGEKPVFDCSS
                     MRHYGSESSTYRGMDLRQPWWHVRGIKIYKAGYNGIIIAGENITVEGMTIEECGMDGI
                     SLGAGAVNTLILNCDSYRNCDVSSRGENGDGFAAKDGTGTVFRGCRAWENADDGWDVY
                     GGDQPILVDSCWSYGNGVNYWPDLISSSSYQGDGNGFKLGGGGGVDGNAPNVVVNSFA
                     FGNVGKGFDQNHNSWGVICINCTGYNNNGMGNFAFQEVPSMGKHVMINNLSYAGTGQN
                     IATGSTETTNSWNLGLIFTDDMFESLNMEDIKVERDDDYRLTDARIMSLFKLKSNNPA
                     IDKGTVQTYIPLKPYYAIPYSGEKPDLGAKEFDQGNWTFPEPSETEPLEPENPENNDR
                     PVGEKTVIVDYPNQFTVEANGGTSVVWGPFSLASAESAVFDMQAVGSSSGTVIIQFST
                     DNQNWVQVGAQAKNSSTSWVTGKVIDLSDPDAPWGPTIYIRFYNNSTRDVNIKNLKIT
                     GTLYILNNINGIDSDYNRIISVRYFDFTGKEIKTIDKEGLYIKQTFFENGKIKSEKIF
                     KKNYFRGN"
     gene            8824..10653
                     /locus_tag="PLR72_01635"
     CDS             8824..10653
                     /locus_tag="PLR72_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35945.1"
                     /translation="MKNLKKIFTILFLFYCNSIFYAQQLTHVIGYPEGFASLNGGVTG
                     GEGGPVVTVNNAAELKDAVDGTNSATIILVKGTITVSGELSARGNKTIVGLGTDARIT
                     GGGFGWNTKSNVIVQNITFEDAPDDLLKINNKCQNFWIDHCTFSDGANTNAGSHDGML
                     DITRGSQYVTLSYNKFFNHDKNILIGHSDSERGDTAIKVTLHHNWFDGTIQRNPRVRY
                     ATVHCYNNYHVNNQTYGIASACDANVLVENCYFKDTQIPLQVGVPGFSPAGYLVARGC
                     YFENCGTPQTNEKDMTGLFPYNYTLDDVMYVPCIAETYSGADKPYRTAENFECEATSP
                     NTPGEDNSNEDGCIWIKNDLFAPDSDVNGWFWFNNETTCGKYIPSMITLLPSSSGTSS
                     TLQIDKVGAGTDGTSGGPGAIIGCIDLAKSATKGQYIGGAVIFQLPSCGEFKLKTSRT
                     GDNLFSVSVATSADGEFTQKYTYSGSKGIAEIDITQYVLSETPIWVKITNGATGSLNI
                     HGVSVKSYEKECPNQTKKIEKNDISKMWYSGNILTFKSEKTCPAEIVIFSIDGKKLDI
                     PFRGWIEAGIENKVKLNLNYPDNSVYIIVMTTNEGKNILKIKK"
     gene            10659..12281
                     /locus_tag="PLR72_01640"
     CDS             10659..12281
                     /locus_tag="PLR72_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK35946.1"
                     /translation="MKKFIIIIAELIFVLNLWGTNYYVSPNGNDANAGTLSSPKFNIS
                     ALVNTVNPGDTIFILPGTYNYSNIITLTKSGTADKRICISGTGYSTAEERPLLNFSSQ
                     GPGDSNRAFFLKAGANYWHLKGFIIKEAGDNGIKIESNWNIIEFIDFDGCYDSGIQIG
                     LAKDSGSNDGSCAYNYILNCDSYNNCDSHKSGGNADGFACKLFCGPGNIFENCRAWNN
                     SDDGWDFYSNTFGVLVLNCRTWHNGDATTLRGNKSGNGNGFKMGGNSTYSRGTNIAVG
                     CIAFDMHYVGGGGKCFDQNDNQDPQVMLNCTTFDNVKGFSMASGSSGHTIKNCIGFNS
                     RDKIISDQDQNITQNNSWQFGPNQKDKQNGGIANYDDFIVDLSNNDIANQLAANPRNV
                     DGSIPDNGFCRLSPSSDCIDKGQVVSYTFMDKTYQIPYTGIAPDLGAYEYQSSTGIFH
                     TIHNNISTNLRITNTQITDIATVLFYLEKTEPISLSITDMSGKKVMNCIDNQIFPSGN
                     NYHIIYLGNLSKGMYILHLNSKNNRQSIKFIR"
     gene            12282..14612
                     /locus_tag="PLR72_01645"
     CDS             12282..14612
                     /locus_tag="PLR72_01645"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK35947.1"
                     /translation="MKIRLFILFGFTFFCFNVIADVIVQAESGLFDGKIDTQHAGYTG
                     TGFVDFTNAVGSKLILEFSIAEEMPSAKVLVRWANGKNDDRAMSVIVNDELQIASVPF
                     GYSGGFTTWVETEITLNLRKGTNRIIFTSLTSNGGPNFDRITIVGATEGVKEYSLTVN
                     IVGKGSVVKTPDEPFYPNGTTVQLEAIPDASKQATFLGWSGDITGTNPKISILIDGNK
                     TVTATFKSSVHSAYYCAPQEKGGNDNNPGTINAPFFNLSKAISVMDAGDTVYMRGGTY
                     RYTATIFLTANGNENERYCIFNYQGEKPVLNFYDIFSNYSNISSTARGEARGFKITGD
                     YYYLKGLEICQAPDNGIKIEGSYNICEQLVLHHNGDSGIQIGLAKDKPDAADKVCCNL
                     VKNCDSYRNLDWGTSYENADGFACKLSPGALNRFVGCRAWENADDGWDFYMTHYTIYV
                     DSCWTMGNGNPDLVATPDPDWEYGQKNTPPTSWSGDGNGFKLGGDDWAAKHVVRNCIA
                     FDGYGTGVCFNENNNADSLFIFNCVGWQGMKNFRVRAYPSDLRNNISFDPKVGGQGQL
                     YDLASGTTEKNNSWNSINGQYPLVPYKTADNKTFDQKSIYNQFVSTSKEDFLAPREPD
                     GSLPKNGFGKLVDNSFFIDKGTSIVRGIHPVTLKRYDFSIPYFGTAPDLGAFEYQTPT
                     GVSQIVSSDPHLKAYPNPFYEATTFDIIAFESGNAQLNISDLTGKIVYATSISGLMQG
                     EKRTIRFNQQLTTGIYLVNFINGKYHQILKLIKLDN"
     gene            14612..17041
                     /locus_tag="PLR72_01650"
     CDS             14612..17041
                     /locus_tag="PLR72_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOK35948.1"
                     /translation="MKKLFFFILTCTFFSTSFGKQYFIANNGNNTNSGTIESPFLGFN
                     AAYSIAQPGDTIWVRGGVYNVSTIWKIEKAGNVNAYYNLWGYTPDIRQGDSVILNISA
                     TNGIRITLDGKYWHIKNLVIQNADDNGMRIEGSYNIIENCVFRYNSDSGLTIQQSNGT
                     MPNDGTIAAFNLILNCDSYYNDKNVSNADGFACKLSPGAGNVFRGCRSWANGDDGFDF
                     YYSRFQIIVEDCWTFRNGLKGGNGNGFKSNGSASSSSTPRTQAPHVYIRCIAFDHKYT
                     AGSNNKGFDQNHNEGNVTMINCLSFDNEKNYAYQEDTQNGKNHVLRNCVGFEPYSETL
                     GRTLLPGEIINPGTKNEISYESSNTWFGNTVDAAYNSWNLAESLNNGWNPSRDDYESL
                     ELADAIAERQADGSLPDNSFGKLKPESQMKNAGISYIWELSLPLSSYPNNPTKPYYVK
                     STLVTTDDGMPDLGPFEIPSAINPFNQSPIVNITAPQAGSEFNLNEVILLQADAMDVD
                     GNIARVEFYVNNNLIGISYTSPYTYDWTCSIAGEYIVMAKAYDNEGAFSNSEGVNITV
                     KNANTEGQLGEKKLLLKLSGTSLTDEIQNKPWSTVNSVSISNNHIKLTYSSSKGGTII
                     SPFIDMSKYLPDSLTLTFASSGGGNRNLNVYVSSDGGATYSAPYLQAGAANNVAVPVK
                     MKLSDKVSGEGNFVLKLEAAGTVEVWNVEVSGYEKIESVISSPAKENVCIPIVSSGKL
                     ILKMNLTELMPFTVDIYNMQGVKMFHQKDENSQKGINEMIININSYPSGMYIIKLNSG
                     KDNFTSKFIKP"
     gene            17060..18895
                     /locus_tag="PLR72_01655"
     CDS             17060..18895
                     /locus_tag="PLR72_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOK35949.1"
                     /translation="MKNGLMILLFFAFSLNLYSVNEIVVAKNGSGDFTTIQNAINSIP
                     TSNNDWKTIVVKNGIYNEHVMIKSNYIALVGESRSGTRIEFSIPRAEWYAANGTNAGT
                     GVINIASNLHDIVIANMYIRNTYEGSEDYTEVIRSETGTTRLWFINLDVLCLWKDTFA
                     PWGKAGGMYYVSDCNFRGSIDAFCPRGYCYAINCRFTETKSSSPIWHEGVATEDQKLI
                     IQGGSVHSEYNKNVKLQNCQKYAKFYYLDVQLSDSITQLGKSATTYFYGVKGPSGLTW
                     FNNNLTLDERKFIDAEWTFDKKWNPERTMPRVLPFASLPQPYNGRYALSAGNLTLKWT
                     SARNAVSNKVYFGTANQLQLLGETTLDSMITPVLETNKTYCWHVNTVTADGEMEGQLW
                     FFSTGDNIDQSIDDNPTTPVEEDNTDYNSPRLNQYDTYLNCKTMPKEPAWIITSGSVN
                     WSQGTGYYWGSSSAVLTLKVNNCTKIECTYLNGSTSRPIIITDGGTNNDITAYPATAN
                     VESTVTFIPKTTDDDDIQVKTSSASGLTLTKIVLYGIKSEIGSVFSTSDTFFTLTSYF
                     IQSDDLMDIFDITGKMVFEKVQHAKLLPGIYIVRSANKVKKILIK"
BASE COUNT         6302 a         2788 c         3645 g         6162 t
ORIGIN      
        1 taaacataaa ttggtaaaat catagtttta attcgataaa atataatttt ttaataatta
       61 gtcacttatg ttatttttat tttttatact ttctaaataa ctttgcaaaa taatggtagc
      121 tgatatttta tcaactaaag ctttattctg tctgtctttt ttctttaaac ctcctttgag
      181 catagctttg tgagctaaag ttgaagtaaa acgttcgtct acataaataa gttcaatatc
      241 tggaaatttt ttttgaaggg aggcaacgaa aggcttaatg tatttcattg attctgattc
      301 ctgcccattc atttggagag gataaccgat caggattttt tcaacatttt cttttgctag
      361 aaatttttcc aaaaatggta ttatttcatg aacatgtact gtttccaaag cgttggccga
      421 aatttgtaaa ggatcggtta ttgctattcc aacccttttc cttccataat caagtgccaa
      481 aattcttccc atttttgagt aattttttac aaaattaagt gataattttc aatttccact
      541 gatattattg gaatttcttt actaactagc aactaaatcc acttttgtat attcaatagt
      601 gtgttatatt tttagttaaa taattgaaaa tcaataaatt tttcaagttt ttatttagct
      661 aatcacttga aaatcaatac ctttataggt tttcgttttc atttttttct aaatattttt
      721 tgacaagcaa tttcttcaaa cgttcgtttt tctttttcaa tttttgagta actttctttt
      781 cacatttaat aatctggcat aagtgccaat acctctcctt cttcatattt ccttttccaa
      841 tcataataag ttgtggttag atactgtgct tacaacaagt ttctgtcgtg ctttatgttt
      901 cggcttcctt cagaattggg agttttcttc tctactccat gcctttttta taatgttaaa
      961 ttaatgtttc attaataatc cagttcgatc atttaaaggg ctgatacaaa agcatgtaag
     1021 atatattaat gtacaattaa taataaataa tttcccaatg ataaatatta caccatttta
     1081 gtcaattatt ccacagtatt tttttgtttc ttaactacat ttggaacaaa aaatttattt
     1141 ttaactttta aaaatttgta aaaaaccatg aaaaaaattt taatgaccgt tattttatta
     1201 acgactatta tgttagtaag ttattctcgc acaatttatg ttgcgactac tggaaatgat
     1261 tccaatcctg gaactgaaac atctccctat ttgaatatcc aaaaagctat agatgctgcg
     1321 gcacctggtg acactattta tatacgtgga gggacttata tgttaactaa acgaattaaa
     1381 atagaaaaag caggaacagc cgatgcaaga atttgtttat ggggatatcc tggagaacgt
     1441 gtaattatag atggtagtca gattgttgcc aatagtacaa atgaattcaa acaagcccga
     1501 tgtatttacg taaatcattt tggtgattac tggcatttta aaaacttaga attgtgcaat
     1561 gctaaagaca atggaatgaa gttggaagga agttataata ttgtggagaa gtgtaaattc
     1621 tatggaaata acgatacagg acttcaaata ggaatgtata aagatttttc tatcgaagaa
     1681 acgcagtctt ttccaatttc tggagagccc caatttaatc cgggctatac ttattgtaag
     1741 tataatattg tcattaattg tgattcttgg tataattgtg atactaaatc atatagtgga
     1801 tcagatgatg gaaaagacgc tgatggattt gctgctaaac tatttcctgg tcctggaact
     1861 gaatttcacg gttgccgtgc atggcaaaat tctgatgata attgggattt gtacatggtg
     1921 tatcatccga tactaattga taattgttgg agttggaaag ctgggtatga taaaaatggt
     1981 acagcattcg ggaatggtaa tggttttaaa cttggaggtg ggggaacttc tggaggagca
     2041 gcttttccac aatcggtagg agcccatgtg gtaaaaaact gtattgcttt tgattgttta
     2101 cacaagggat ttgatcaaaa caatgcgtat gaagctatgt acttatttaa taacgtatca
     2161 tggaataatg agtacaatta ccgtttccct acaattttta tgtatggtac catgtatatg
     2221 agaaataata ttggttttaa acccgtaaca cttaatcatg aatttttatc ggcagacaaa
     2281 ccgggttctc aagtacctaa tacggcgtat aatagttgga caactattga tggctgcgat
     2341 ccatataaag atgggaataa agtaaatggt gtaaaagttt acgcaaaaga ccattctgca
     2401 caatttaaaa gtttatcatc tacgcttttt ttggctgata ggcaagttga tgggagtctc
     2461 cctgaaaatg atttttgtaa attagttgaa ggaagtatat ttattaatgc aggtgaaaat
     2521 attgaaaact ttgttccgca agcacatagt cctggtggat taacattacc tcctgttaca
     2581 atattgtata atgacggaag agcagatatg ggagcgtttg aaacaggaga gcctaccatt
     2641 gcaactttat cgcttatttc agggaaagca gatcaatttg tatatgttgg aactgctatt
     2701 ataaatacag tttataaatg gggtggcgct gctactgatg taattgttag ggacttacct
     2761 gatggtttaa atgccataaa agatatgact gcaaaaacag taaccatttc tggtattcct
     2821 actgttgaag gaacttattc tattgaaaat ataggaggta ctaacactat tgttttaaca
     2881 ggcaatatcc gcatatctaa tattgcacct gctacattaa ctattacaca aggtcatgct
     2941 atacaagaag tatttttcgg tgagccaatt gaaaccatag tatttacttg gggtggtgga
     3001 gctacagata ttacttatac cgctcttcct gcaggtctta ctgcaacaaa agacatagag
     3061 aacaaaactt taaccatttc aggtgttcct actactgatg gttcattcac agtaactaca
     3121 ttaggcggaa tggaaggatc acaagttaca attaattgta atatcatccg tgtattacga
     3181 acaaaagttc ttactggcga ctggtatcat atacaggatc cttatgaaaa tagacctgct
     3241 gatttacagg gcaatgttgt aacatttgca acaggatcta attcctcata tccaactgtt
     3301 tgggatccaa cctatgttga gagtacaggt tcggctcctt caggttgtac accaggagct
     3361 ataaatgtag aaaggaatgg aggaagtgtt acatggaatc tgccaagctt ggtagaaatg
     3421 aaatctaata ttcactttac aggaacaaga actttgaaaa tagaatggac aatagatggt
     3481 gttacaaaaa catggacttc tgcatcgttg agcaaacgaa caatgttgaa ttgggatatg
     3541 atggcagata ttggtttgga accaacaaca aaacctgtaa caattaaatt tatcaatact
     3601 tcaacaagtg gaggcattcg catatatgat ttttatataa aagtttatga agatcaagtt
     3661 tcaggaattg aagaggtaaa agttcaaact aacaaattca atatgtatca aacagaaaca
     3721 gcgttaattg tatcaggtga tattgctaaa ttaacagttt attcattatc gggaagtgta
     3781 gtagcacaat ctgaaatgtc gcaagttgtt aagataaatc atttgaatca aggcgtttat
     3841 gttgttcaca taactgacaa agaaggttgt caagttactc aaaaatttat caaaaaataa
     3901 taatataata atcagggaat aaagtgtttt gaaaaacgct ttattcccat ttacatttta
     3961 taattaattt taaaaatacc taaatgaaaa acaaattatt ctttgtaact cttatctttt
     4021 ttttcttcgg ctttttactg agtgctcaaa atactattgt agaaaatttt gaaacgggtt
     4081 tacctacaag tgcaccttca agtgaaactt ctgttgctct ttcaacgggt atttggaaaa
     4141 taaaaggagt ttttgcaaaa accgacaatg gaagtattag attggctatg aacacaaatg
     4201 gttacgctat tacgccacca attgataagc cagtatctgt ttcttttaaa catcggggta
     4261 gtggtagtgg taaagtgctc actgtacaaa aatctattga tgacggaagt tcctggacga
     4321 ctattggaac tgctacggta agctcttctt ctacttataa caccgccaat atctctatta
     4381 atgaacaagg aactataggt gttttactca aatttatttg tggttcgagt accatttata
     4441 ttgatgatgt aactattgtt tgttcaagta tagtagatga accaacaatt caagcgtcta
     4501 tctctgcttc agaaataact gctacatcaa tgaagattaa tttcaataaa gggaatggcg
     4561 aaggtcgctt attggtatat tctaaaggag aagaagtaaa ctggttaccg gaagatggga
     4621 tcgcttatac taatttacct aaagttttaa ctgataatgt gataggtgta tattctggta
     4681 atgttgattt tgttacagta acaggacttg aagcaggtga aacttactat ttttatgtat
     4741 ttgaatataa cggagctaat gatgctatta attatcttac tcagccagta ggaaaattaa
     4801 ctcaaagaac tgtagaagca ccaagtatta caacaagtgt taatgctatt aattttggaa
     4861 gtgtaaaaat aggtatttca tcaaaacgca gttttactct tttagctaaa tatttaactc
     4921 cagaaaatgg aacaatcaca ttgacttctt caaatgattt tctgatatca ataaattctt
     4981 caagtggatt cggtacttct cttactttaa attattcttc agctacttta ttatccacta
     5041 ctatttacgt tcaattttta ccttctgaat taaagtctta ttcacaaatt ctttctatct
     5101 caggaggtaa tgctgaagcg caggtacaac ttatcggtat tggttcaaat acggatgcaa
     5161 aagtttattt tatttcacca tcaggaaatg ataatgctga aggaacattt gatgctccgt
     5221 ggtataattt gcaaaaagct atcgacgcag ctttaccggg agatacaata ttagttcgcg
     5281 gcggtactta ttacccaaca atgatgaaag atggtacgaa aactactata cgtataaatt
     5341 catcaggtac agctgacaaa agaattacta taaaaaatta tccaagcgaa ttttctatac
     5401 ttaattttaa agatcaacct aaaaaacaag gtattcgtgg agttcaacta aatggagatt
     5461 attggcatat tataggattg catattacgc aagcaggaga taatgccata aaattagaag
     5521 ggaatcataa tattatcgaa aaatgtactt tcagttataa tgatgataca ggtattcagt
     5581 taggatttgg tcataatttt tctgatacac atcctggtat ttcttcaaat gatggaagct
     5641 attgtgctta taatgatatt attgattgtg actcatactt gaattgtgat gcagataatt
     5701 ttggcagtga tgccgatgga tttgcttgta aaatgcataa tggcaaaggg aatcgtttta
     5761 ttcgttgtcg ttcatgggat aatgcagatg atggatggga tttatatgaa acagattatc
     5821 cagtcatatt aattgaatgt tgggcttggg gttctggaag agctgaaaat tttgattggg
     5881 ttcaagcctc aggatctttc cagggaaacg gcaatggaat aaaaatggga ggaaatggta
     5941 caggtggttc atcgaaagga aaacatgaag catggaactg cattgctttc aattgcaata
     6001 aaacaggttc agttaaaggt ttcgaccaga acagtcatgg agatggagaa aaattggtaa
     6061 actgtcttgc atttggttgt ggatatgatt ttatgtttga aaaatcagga accaactgtg
     6121 aatattataa taatgtttgc tttggtaata ttgaaattgc ttcaggctgc acagaaagca
     6181 acaatgccat gttgtcaacc tctaccaaag catggaaaaa taatgttatt cgtggctttt
     6241 ctagttctga ttatgtaagt ttaagtgaag atgatgccaa agctccgaga ggggcagatg
     6301 gtagtatgcc taaaagattt gcacgtttaa aaagtggtag tgtattagta gataaaggat
     6361 tatcttataa tgttccctat acagacgaat ttccattttt gtttcaacca atttatggtt
     6421 tagcacgtga tttaggtcct tatgaacttc aagaaggtgt aatttctgga atgcaaatca
     6481 ttataaatcc aaaagcagaa tttaatttgt cgattttatc gaatccatgt aaatcggaag
     6541 caatgattaa atttagctct gatttttcag gaatagcttc cttaaaaatt gtcgctttag
     6601 atggtcacgt gatagaagaa tgttataata aaaatgttga agctggagta gaatatttcg
     6661 tttctatttc tgtaaataat ttacagaatg gaatgtattt atgtgtttta agtataggag
     6721 gtcaaactaa aacagcaaaa cttattgtag caaaataagt ttattaacag ttaacctttt
     6781 actggataat tcttttaaat aaatctaaca aattttttaa aaaaatatct aataaaatta
     6841 aataaatatt caaccatgaa aaaaagtttt ttattatatt atgtattgat cctgatttct
     6901 gtattttcta caaatgcttt tggacgaaaa tattatatat caccaaccgg tagtgatagc
     6961 aatgatggac tcacactaca aacagcatgg ggaactccga actttgcttt tcaaacaatc
     7021 caaagcggtg acaccttgtg ggtaaaagga ggaacttata tagtaaattc tacagtaaaa
     7081 gccaaaaatg gtggaacaaa aactgctccg gtctgtgtat ttgctatacg aggtgaaaaa
     7141 cctgtttttg attgttcttc tatgcgtcat tatggaagtg aaagttccac ttatcgaggt
     7201 atggatttac gtcagccatg gtggcatgtt cgaggtataa agatttataa agctggatat
     7261 aatgggatta ttattgcggg tgaaaatatt acagtcgaag gaatgaccat tgaagaatgt
     7321 ggaatggatg gaatttcgtt aggtgctgga gctgtcaaca ccttgatttt aaattgtgat
     7381 tcttacagaa attgtgatgt ttcttcaaga ggagaaaatg gagatggatt tgctgcaaaa
     7441 gatggaacgg gaacggtgtt cagaggttgt cgtgcatggg aaaatgccga tgatggctgg
     7501 gatgtttatg gaggtgatca gccaattttg gtagatagtt gttggtcgta tggaaatgga
     7561 gtaaattatt ggcctgattt aatatcaagc tcttcttatc aaggtgatgg aaatggattc
     7621 aaacttggtg gaggaggagg ggtggatgga aatgctccaa acgtagtagt aaatagtttt
     7681 gctttcggaa atgttggaaa aggttttgat caaaatcaca attcatgggg agttatctgt
     7741 attaattgca cgggatataa taacaatgga atgggaaatt ttgcattcca ggaagttcca
     7801 agtatgggta aacatgtaat gatcaacaat ctttcatatg cgggtacagg acaaaatatt
     7861 gctacgggta gtactgagac caccaatagc tggaatttag gacttatttt taccgatgat
     7921 atgtttgaaa gtctgaatat ggaagatata aaagtggaac gtgatgatga ttatcgttta
     7981 acagatgctc gcattatgtc gctttttaaa cttaaaagca acaatcctgc gattgacaaa
     8041 ggaacagtac agacttacat tccattgaaa ccatattatg ctattcctta ttcgggcgaa
     8101 aaaccagatt taggtgcaaa agaatttgat caaggtaatt ggacttttcc tgaaccttca
     8161 gaaacagagc cattagaacc cgaaaatcca gaaaataatg atagacctgt tggtgaaaaa
     8221 accgttattg ttgattatcc caatcagttt actgttgaag caaatggagg aacttcagtg
     8281 gtgtggggtc cattttctct ggcttcagca gaatctgctg tttttgacat gcaagcagta
     8341 ggttcttcta gtggcactgt aataatacaa ttttcgacag acaatcagaa ttgggttcag
     8401 gtaggagcac aggctaaaaa tagttctaca agctgggtta caggaaaagt tattgatctg
     8461 agtgatcctg atgctccatg gggaccaacc atttatattc gtttttataa taattcaaca
     8521 cgcgacgtca atattaaaaa tttaaaaata acaggtacat tgtacatttt gaataacatt
     8581 aatggaattg attctgatta taatagaatt atctctgtcc gatattttga tttcactgga
     8641 aaagaaataa agacgataga taaagaagga ttatatatta aacaaacctt ttttgaaaat
     8701 ggaaaaataa aatccgaaaa aatcttcaaa aaaaattatt ttagaggtaa ttaattattt
     8761 tttaaaatat tataaattct tgtaatcata tttatataat aacttataaa aactaatact
     8821 gaaatgaaaa acttaaaaaa aatatttaca attttatttt tattttattg taattcaata
     8881 ttttatgcac aacagttaac tcacgttatt ggatatcctg aaggatttgc ttcacttaat
     8941 gggggtgtaa caggtggtga aggtggaccc gttgttacag ttaataatgc agccgaatta
     9001 aaagatgctg ttgatggaac caattcagca acaattattc tggtaaaagg aacaattact
     9061 gtttcaggtg aattatccgc tcgtggaaat aagactatag ttggtttagg aaccgatgca
     9121 cgaattacag gtggaggatt tggatggaat acgaaatcga acgttattgt tcaaaacatt
     9181 acttttgaag atgctcctga cgatttactc aaaataaaca ataaatgtca gaatttctgg
     9241 atcgaccatt gtacatttag cgatggagct aataccaatg caggttcaca tgatggaatg
     9301 ttggatatta cgcgaggcag tcaatacgtt actctttcat acaataaatt ttttaatcac
     9361 gataaaaata tccttattgg acacagcgac agtgaaagag gtgatactgc aataaaagta
     9421 actcttcatc ataattggtt tgatggtact attcaacgta atccgcgagt aagatatgcc
     9481 actgttcatt gctacaacaa ttatcatgtt aataatcaaa catatggaat agcttcagca
     9541 tgtgatgcca atgtactagt agaaaattgc tatttcaaag atacacaaat tcctttgcaa
     9601 gttggtgtcc cgggattttc acctgctggt tatttggtag caaggggctg ctattttgaa
     9661 aattgtggaa ctccccaaac gaatgaaaaa gacatgacag gtttgttccc gtataattat
     9721 acactggatg atgtaatgta tgttccctgt attgctgaaa cctattcagg tgctgataaa
     9781 ccttatcgca cagcagaaaa ttttgaatgc gaagctactt ctcctaatac tcctggagaa
     9841 gataattcaa acgaagacgg ttgcatttgg attaagaatg atttgttcgc acctgattct
     9901 gatgtaaatg gttggttttg gtttaacaat gaaaccacat gcgggaaata tattccttcc
     9961 atgatcactc ttttaccgtc atcatcgggc acaagttcaa ctttacaaat cgataaagtt
    10021 ggggcaggaa ccgatggaac atcaggaggt ccaggagcta ttatcggttg tattgactta
    10081 gcgaaatctg ctactaaagg acaatatata ggtggtgctg taatttttca acttccaagt
    10141 tgtggtgaat ttaagttaaa aacttcccgt acaggggata atttatttag cgtgtctgtt
    10201 gctacttcag ccgatggcga atttacacaa aaatatacct attcgggtag taaaggtatt
    10261 gcagaaattg atattactca atatgttctt tccgaaacac ctatttgggt aaaaataacc
    10321 aatggagcca caggaagttt aaatattcat ggagtttctg ttaagtctta cgaaaaagag
    10381 tgtccaaatc agacaaaaaa aatagaaaaa aatgatattt cgaaaatgtg gtattctgga
    10441 aacattttga catttaagtc tgaaaaaact tgtccagcag aaattgttat tttttctatc
    10501 gatggaaaaa aattagatat tccttttcga ggttggatag aagccggtat cgaaaacaag
    10561 gtaaagttaa atctaaatta tcctgataat agtgtttata tcattgtaat gacaaccaat
    10621 gaaggtaaaa acatactgaa aattaagaaa taaagcttat gaaaaagttt attattatta
    10681 tagcagaatt gatttttgtt ttgaatctat gggggacaaa ttattatgtt tcgcccaacg
    10741 gaaacgatgc gaatgctgga acattaagtt ctcccaaatt taatatttca gcattagtaa
    10801 atacagtaaa tcctggagat acaattttta tattacctgg tacttataat tattccaata
    10861 ttattactct cactaaatct ggaacagccg ataaaagaat ctgtatttct ggtactggat
    10921 atagtacagc cgaagagcgt ccactgctca attttagttc acaaggacct ggagattcca
    10981 atcgtgcctt tttcttgaaa gcgggagcaa attattggca tcttaaaggg tttattatta
    11041 aagaagccgg agataatgga attaaaatcg aaagcaattg gaacattatt gaatttatag
    11101 attttgatgg ttgttatgac agcggaattc aaatcggact tgctaaagat tcaggatcaa
    11161 atgatggttc atgtgcctac aattatatat tgaattgtga ttcatataat aattgtgatt
    11221 cacataagag tggaggcaat gcagatggtt ttgcctgtaa attgttttgc ggaccaggaa
    11281 atatttttga aaattgtcgc gcatggaata attcagatga tggctgggat ttttattcca
    11341 atacttttgg ggtattagta ttgaactgtc gtacatggca taatggggat gcgactactc
    11401 ttcgcggtaa caaatcaggt aacggaaatg gtttcaaaat gggtggtaac agtacttatt
    11461 cacgtggaac caatatagct gttggttgta tagcttttga tatgcactat gttggtggag
    11521 gtggaaaatg ttttgaccaa aatgacaatc aggatcctca agtaatgctg aattgtacaa
    11581 ctttcgataa tgtaaaaggt ttctcaatgg cgagtggaag tagtggtcat actatcaaaa
    11641 attgtatagg atttaattca agagataaaa tcatcagtga tcaagatcaa aatattaccc
    11701 aaaataatag ctggcaattt ggacctaatc aaaaagacaa acaaaatgga ggcattgcca
    11761 attatgatga ttttatagtt gatttaagta ataatgatat agccaatcag cttgcagcta
    11821 atcctcgcaa tgttgatgga agtattcctg ataatggttt ttgcaggtta agtccttctt
    11881 ctgattgtat tgataaaggt caggtggtaa gctatacttt tatggataaa acttatcaaa
    11941 ttccttatac tggtatagct cccgatttag gcgcttatga atatcaatct tctacaggaa
    12001 tttttcatac tattcataat aatatctcta ccaatttaag aattacaaat acccagatta
    12061 ctgatatagc tactgtttta ttttacttgg agaaaactga accgatttct ctttctatta
    12121 cagatatgtc aggtaaaaaa gtaatgaatt gtattgacaa tcaaattttt ccatcaggga
    12181 ataattatca cattatttat ctgggaaatc tttcaaaagg aatgtatatc cttcatctta
    12241 attctaaaaa caaccgtcaa tcaattaaat ttatacgata aatgaaaata cgtttattta
    12301 ttttattcgg atttactttt ttctgtttca atgtaattgc tgatgtaatt gttcaggctg
    12361 aaagcggact atttgatggc aaaatagaca cccaacatgc tggctacaca ggaactgggt
    12421 ttgtcgattt taccaatgca gttggaagta aactgattct tgaattttct attgcagaag
    12481 aaatgccttc tgctaaagtg ctggtacgct gggcaaatgg gaaaaatgat gatagagcta
    12541 tgagcgtaat tgtaaatgat gagctacaaa ttgcaagtgt tccttttggt tattcaggtg
    12601 gttttacaac atgggttgaa actgaaataa ccttgaactt aagaaaagga accaacagaa
    12661 taatttttac ctcattaacc tctaatggag gtccaaattt tgacagaatt accattgttg
    12721 gggcaactga aggagtaaag gaatattcat taactgttaa tattgttggc aaaggaagcg
    12781 ttgttaaaac tcctgatgag cctttttatc caaatggaac aacagtccag ttggaagcaa
    12841 ttcctgatgc tagtaaacaa gccacttttc tgggctggtc aggtgatatt acaggtacaa
    12901 accctaaaat ttctattcta attgatggta ataaaacagt aacagccact tttaaatcat
    12961 ctgtgcatag tgcttattat tgtgctcccc aagaaaaagg tggaaatgat aataatccag
    13021 gaactattaa tgctccattt tttaacttat caaaagcaat tagtgtaatg gatgcaggcg
    13081 atacggttta tatgcgaggc ggaacatacc gttacactgc aactattttc ctgactgcaa
    13141 atggaaatga aaacgagaga tattgtattt ttaattatca gggtgaaaaa cctgttttaa
    13201 atttctatga tatttttagt aactattcaa atataagttc tactgcacga ggtgaagctc
    13261 ggggttttaa aattactgga gattattatt atttaaaagg tcttgaaatt tgtcaggcac
    13321 ctgataatgg aataaaaatc gaaggaagtt ataatatttg tgaacagctt gttttacacc
    13381 ataatggcga tagcggaatt caaatcggac ttgcaaagga taaaccagat gctgctgata
    13441 aagtatgttg taatttggtt aaaaattgcg attcatatcg taatcttgac tggggaacaa
    13501 gttatgaaaa tgccgatggc tttgcttgta agctttctcc cggtgctctt aatcgttttg
    13561 ttggttgccg tgcatgggaa aatgctgacg atggttggga tttttacatg acccattaca
    13621 ctatttatgt tgattcttgc tggacaatgg ggaatggcaa cccagactta gttgcaactc
    13681 ccgatcccga ttgggaatat ggacagaaaa atactcctcc cacttcatgg agtggtgatg
    13741 gaaatggttt taaacttggc ggagatgatt gggcagcaaa gcatgtggtt agaaattgta
    13801 ttgcttttga tggatatgga acaggagttt gtttcaatga aaataataac gctgacagtt
    13861 tatttatttt taattgtgtg ggatggcaag ggatgaaaaa ctttagagta agagcttatc
    13921 caagtgatct aagaaataat atcagttttg atccaaaagt aggtggtcaa ggtcaattat
    13981 atgatttggc aagtgggaca accgaaaaaa ataatagttg gaacagtatt aatggtcaat
    14041 atcctttagt cccgtataag acagccgata ataaaacatt tgatcaaaaa agtatttata
    14101 atcagtttgt gagtacctca aaagaagatt ttcttgctcc tcgtgagcca gatggaagct
    14161 taccaaaaaa tggctttgga aaattagtgg ataattcatt ttttattgat aaagggacga
    14221 gcattgttcg aggaattcat cctgtcacat tgaaacgtta tgatttttca attccttatt
    14281 ttgggacagc acctgatttg ggagcttttg agtatcaaac accaactggc gtttctcaaa
    14341 ttgtttcttc tgatcctcat ttgaaagctt atccaaatcc tttttatgaa gcaactactt
    14401 ttgatattat agcttttgaa agtgggaatg ctcaattaaa tatttctgat ttgacaggta
    14461 aaattgttta cgctacctct atttcaggac taatgcaagg agaaaagaga acaataagat
    14521 tcaatcagca attgactact ggaatttacc tggttaattt tattaatgga aaatatcatc
    14581 aaatactgaa acttattaaa ttagataatt aatgaaaaag ttatttttct ttatattaac
    14641 ctgtaccttt ttttccacaa gttttggaaa acagtatttt attgctaaca atggaaataa
    14701 cactaattca ggcacgattg agtcgccttt tcttggattt aatgctgcat attcgattgc
    14761 acaacctggt gacactattt gggttcgcgg gggagtgtat aatgtaagta ctatctggaa
    14821 aatagaaaag gcagggaatg taaatgctta ttataacctt tggggttata cacccgatat
    14881 tcggcaagga gattcggtaa tactaaatat aagtgctacc aatgggattc gtattacttt
    14941 ggatggcaaa tattggcata ttaaaaattt agttattcaa aatgctgatg ataatgggat
    15001 gcggattgaa ggaagttaca atattattga aaattgtgtt tttcgttata attctgatag
    15061 tggattaacc attcaacaat ccaatggcac gatgccaaat gatggaacta ttgcagcttt
    15121 taatttgata ttgaattgtg attcatatta taacgacaaa aatgtgagta atgcagatgg
    15181 ctttgcttgt aaactttcgc caggtgcagg aaatgtattt cgtggctgtc gttcgtgggc
    15241 aaatggcgat gatggctttg atttttatta ttctcgtttt caaattattg tagaagattg
    15301 ctggacattt cgaaatggct tgaaaggagg gaatggtaat ggctttaagt caaatggaag
    15361 cgcaagtagc tcttctactc cacgaacgca ggctcctcat gtatatattc gctgcatagc
    15421 attcgatcat aaatatacag ctggttcaaa taataaaggt tttgaccaga accataatga
    15481 ggggaatgta actatgatca attgcctttc ttttgacaac gaaaaaaatt atgcttatca
    15541 agaagatacc cagaatggga aaaatcatgt tttacgtaat tgtgtaggtt ttgaacctta
    15601 tagcgaaaca cttggacgta cccttttacc aggcgaaata attaatccag gaacaaaaaa
    15661 tgaaattagt tatgaaagct cgaatacgtg gtttggtaat acagtagatg cagcatacaa
    15721 ttcgtggaat ctggctgaat ctttaaataa tggttggaat ccttcgagag atgattatga
    15781 aagtctcgaa ttggctgatg ctattgcaga acgccaagcc gatggttcat tgccggataa
    15841 tagttttgga aaattaaaac ctgaaagtca gatgaaaaat gctggcattt cctatatatg
    15901 ggaattaagt cttcctctca gctcttatcc caataatcca acaaaacctt attacgtaaa
    15961 aagtacactt gtaacaactg atgatggaat gccggattta ggtccttttg aaattccttc
    16021 tgcaataaat cctttcaatc aatcacccat tgtaaatatc acagctccac aggcaggaag
    16081 tgaatttaat cttaatgagg taattctttt acaagctgat gcaatggatg tagatggcaa
    16141 tatagcaaga gtagaatttt atgtaaataa taatttaatt ggaatttcct atacttctcc
    16201 ttatacatat gactggacat gttctattgc aggcgaatat attgtcatgg caaaagctta
    16261 tgataatgaa ggcgcattca gtaattctga aggtgtaaat attactgtga aaaatgcaaa
    16321 tacagaagga caacttggag agaaaaaatt attattgaaa ttaagtggta caagtttaac
    16381 tgatgaaatc caaaataagc cgtggtccac agtaaattct gtatccattt caaataatca
    16441 cataaaactg acttatagta gtagtaaggg tggaactatt atttctccat ttattgacat
    16501 gtccaagtat cttcctgatt cattgactct tacgtttgcc agttcaggtg gaggtaatag
    16561 aaatctaaat gtttatgttt cgtctgatgg aggcgctact tacagtgctc cttatttgca
    16621 ggcaggagct gctaataatg tggctgttcc agtaaaaatg aagttatcag ataaagtgtc
    16681 tggtgaaggt aattttgtgt taaaactgga agctgctggt acagttgaag tatggaatgt
    16741 ggaagtttca ggttatgaga aaattgaaag tgtaatttct tcaccagcaa aagaaaatgt
    16801 ttgtatccct atagttagtt caggaaaact gattttgaaa atgaatttga cggaattaat
    16861 gccattcact gttgatattt acaatatgca aggagtcaaa atgttccatc agaaggatga
    16921 aaattcccaa aaaggaataa atgagatgat tatcaatatt aattcttatc catcaggaat
    16981 gtacataata aaattgaatt cagggaaaga taatttcact tcaaaattta taaaaccttg
    17041 aaaatccaat agatatttta tgaagaatgg attgatgatt ttattgtttt ttgctttttc
    17101 attgaatctc tattcagtaa atgaaatagt agtggcaaaa aatggttctg gagattttac
    17161 caccatacag aatgctatta actcaattcc tacatctaat aatgattgga aaaccattgt
    17221 tgtgaaaaat ggaatttata acgagcatgt gatgataaaa tcaaattata ttgcattggt
    17281 tggtgaatcc cgttccggta ctcgtattga attcagtatt ccacgtgcag aatggtacgc
    17341 agcaaatgga acgaatgccg gaaccggtgt tattaatatt gcttctaatc tccacgacat
    17401 tgtgatagca aacatgtata ttcgcaatac ctatgaaggt tcagaagatt acacggaagt
    17461 tatccgcagt gaaacgggaa ctacacgtct ttggtttatt aatctggatg tgctttgttt
    17521 gtggaaagat acctttgcac catgggggaa agctggagga atgtattatg tttccgattg
    17581 caatttcaga ggaagtatag atgctttttg tccacggggc tattgttatg ccatcaattg
    17641 tcgttttact gaaaccaaaa gcagcagtcc catttggcat gagggtgttg caacagaaga
    17701 tcagaaattg ataattcagg gcggttcagt tcatagtgaa tataacaaga atgttaaact
    17761 tcaaaattgt cagaagtatg ccaagtttta ttatctggat gttcagctct ccgatagcat
    17821 tacgcaattg gggaaaagtg ctacaactta tttttatggc gttaaaggac catccggatt
    17881 aacttggttt aataataatc tgacgctgga tgaaaggaaa ttcattgatg cagaatggac
    17941 tttcgataag aaatggaatc ctgaaagaac aatgcctcgc gttttgccat ttgcttcctt
    18001 acctcagcct tataatggcc gttatgcact ttctgctgga aatctgacgc tgaaatggac
    18061 aagtgctcgc aatgcagttt ctaataaggt gtattttggc actgcaaacc aacttcaact
    18121 attaggcgaa actacgttgg attcaatgat tacgccagta cttgaaacga acaaaactta
    18181 ttgctggcat gtaaatacag taactgctga tggtgaaatg gaaggtcaat tgtggttctt
    18241 ttcaacggga gataatattg atcaatcaat tgatgataat cctacaacgc cagttgagga
    18301 agacaataca gattataatt ctcctcgtct taatcaatat gatacttatc tgaattgtaa
    18361 aacaatgcca aaagagcctg cgtggattat tacatcaggc agtgtaaact ggtcacaagg
    18421 gacaggttat tattggggaa gctcctctgc tgtacttact ttgaaagtaa ataattgtac
    18481 caaaatagaa tgtacttacc tcaatggcag tacttccaga ccaattatta ttactgatgg
    18541 aggaacaaat aatgatatta ctgcttatcc tgcaacagct aatgtagaat caacagtaac
    18601 atttattcca aaaacaaccg acgatgacga tattcaagta aaaacaagca gcgccagtgg
    18661 cttgactttg actaaaattg ttttgtatgg gataaaaagt gaaattggat cagtattcag
    18721 tacttcggat acttttttta ctcttacatc ctattttatt caatctgacg atttgatgga
    18781 tatttttgat attactggaa aaatggtttt tgaaaaagtt caacacgcaa aactactgcc
    18841 cggaatttat atcgttcgtt cagctaataa agttaagaaa attttgataa aatgaaa
//
LOCUS       DAOSAS010000019        18827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_2046_length_18827_cov_12.219795, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000019 DAOSAS010000000
VERSION     DAOSAS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18827
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2046_length_18827_cov_12.219795"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..719
                     /gene="udk"
                     /locus_tag="PLR72_01660"
     CDS             84..719
                     /gene="udk"
                     /locus_tag="PLR72_01660"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOK35950.1"
                     /translation="MLIIGIAGGTGSGKTTVVQKIMERLPKSEVVVLPQDAYYRDTSF
                     LPLEERLEINFDHPDSIEFELLIKHLKDLKEGKSIEMPIYSYLTCSRQNETITIHPCD
                     VVMVEGILVLSQPELRKLMNLKVFVDADADDRLIRVIYRDITERGRTVNKVIERYEKT
                     VKPMHEEFIEPTKRFADLIIPQGGNNNIAIDILTKYIENNLRLNHNSNLEN"
     gene            826..1536
                     /locus_tag="PLR72_01665"
     CDS             826..1536
                     /locus_tag="PLR72_01665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOK35951.1"
                     /translation="MFLDIETVPQVANFSDLPDELAHLWEEKFHHLRKHLPEKYNEKT
                     TASEAFNTSAGIYSEFGKIICIAVGFIHFKEEEMFLRTKYFINEDEKELLMEFSQLIE
                     KFGSSKENRLCGHNIKEFDVPYICRRMLINNLPLPSIFQLSGKKPWESSLIDTMELWK
                     FGDYKNFTSLKLLTAVFNIPTPKDDIDGSEVAQVYYKEKNIQRIAQYCTKDVIATAQV
                     FLRLNGFPPISAENIENL"
     gene            1546..2922
                     /locus_tag="PLR72_01670"
     CDS             1546..2922
                     /locus_tag="PLR72_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOK35952.1"
                     /translation="MKNLYLIIFNLFLILTFVSCKKNEIDKRAQISNRDLEQIILSDT
                     LKVTTMYGSTSYFLFRDEDMGFDYEMAENLADFLKVKLKVELANSEPQMIQLLKDGKV
                     DIVAYNIPETKELKKDFQYVFQQPRNSMVIVQRMGRNALSSVVELKDKKVYVKNNSVF
                     HQRLKDLNNEIGGGIQIITVPDSITNDELIEMVANSKIDFTVAYRDKALLHQSYFRRL
                     DCRLTIGFEQNNGWLIRKTSKNLLTAIKEWESLQKTQDKKNRLLTKYWNKSPYFVIHK
                     IKIPKGAISPYDDLFKKYAPTIGWDWRLLAALAFHESRFDSAAISRVGALGLMQLMPQ
                     TAAQFGLDKSDIFNPEKNIEASIQYIKSLNMAFSQVVDKNERIKFIIASYNSGPSHIL
                     DAMALAQKYGKNPHIWFDNVEVYLLEKRNPEYYNDPVVKKGYYRGTETVRYVENVLDT
                     YEKYLKRK"
     gene            3021..3239
                     /gene="tatA"
                     /locus_tag="PLR72_01675"
     CDS             3021..3239
                     /gene="tatA"
                     /locus_tag="PLR72_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOK35953.1"
                     /translation="MNPLLFNLNGWEIPIILLVILLLFGGKKIPEFMSGLGKGIRSFK
                     KGLNDIEEEIKADPDEKKPNDNKSSSSK"
     gene            3336..4145
                     /gene="tatC"
                     /locus_tag="PLR72_01680"
     CDS             3336..4145
                     /gene="tatC"
                     /locus_tag="PLR72_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOK35954.1"
                     /translation="MKEKEMSFWEHLEEFRWTLIRIIIAIAITTIIGLIFIPKIFDSV
                     ILAPCSSNFVLYRFFEKLSGYFPFLLNYSSSSFHVKILNINMATQFMTYMSASLAFGF
                     IFSIPYVLYELWKFISPALYEKETKGVKKAFLLGGVMFLIGCLLGYFIIFPLIFRFLI
                     TFKLSNNIENMISLDSYMRNFYTLILLMGVVFELPLLFWLLSNIGLITRSFFKKYRRY
                     AIIVSLVLAALITPSGDPFSLFVVAIPLYFLWEFSALLVKKDNFEQDISTL"
     gene            4195..4833
                     /locus_tag="PLR72_01685"
     CDS             4195..4833
                     /locus_tag="PLR72_01685"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HOK35955.1"
                     /translation="MENDFIKTVTHTIKHWYIPLIIGILFIVIGIFCFISPMVSYFTF
                     SLIFSVSFLFSGILEIIFSITNRKETDNWGWTLAFGIITFIIGLLLVLNPRITMLSLP
                     YFIGFLMLFRSIFAMSLAIDLKNYYVLNWGYLLAIAILGIIFSFILLLNPLFGGIAIT
                     LLLGFTFLASGCYSIYLSFELKRLKKYADKIPDELKERYESIKREIQEKIWE"
     gene            complement(4934..6184)
                     /locus_tag="PLR72_01690"
     CDS             complement(4934..6184)
                     /locus_tag="PLR72_01690"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOK35956.1"
                     /translation="MKRNLFLIPLFFVMSLHSQKIYHTQILHPTIKTLQIGLSGQNYS
                     LPIIQLNSKDILEIQFDELSHNIHNFTYKVFHCNTDWTLSNLSTNEYLEGFTTGNIYD
                     YALSQNTTYQYTHYRFILPNEEMNFKISGNYMVMIYEENKMENPVAIACFSVVEPQVN
                     IIARLRANTDIELSGRFQQLDIEVGLNGYRLQDPQSEIKMVIRQNNRIDNQVTNILPT
                     YLSTEKLSYINQKALIFEGGNEYRHFDMSSIYVVGEGLDTIRFNKTFYDVYLKEDIPS
                     SSKNYLSQPDLNGKFIIHYQEAFDDINTEADYMQVHFSLYTGQPYFDGQVYIGGEYNY
                     NLLNETSQMQYNFDEGRYNKTILLKQGGYNYQYWFLPKGGKKASVEKIEGSYWQTNNE
                     YTIYVYHRPWGGRYDKLIGVKVMN"
     gene            6488..7870
                     /locus_tag="PLR72_01695"
     CDS             6488..7870
                     /locus_tag="PLR72_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOK35957.1"
                     /translation="MANLIKTTRGLDIPIEGEAQKTLGGTVISDVVALVPEYYHGVVP
                     KMLVKAGDEVKAGSPIFHDKTFDTMNFVSPVSGKVLAINRGERRKILSIAIERDSKIQ
                     YVPFKIKPLSETTGEEVKAQLLNAGLWPFIKQRPYDIIADPNKSPKAIFISCFDSSPL
                     APDYNFILKGQFGDFQTGIDALSKLTKGKIHLGIKPTKDFNDFRSVRNVEITEYEGPH
                     PIGNVGVQINHVDPINKGEIVWTINVQHVLFIGRFFNKGIVDLSYTLALTGPCVVQPQ
                     YYRTLVGANIEPFIKGNVYKGLILRYITGNVLSGIKIEENEWLNPYASQIFVLEEGSD
                     QHEFLGWAMPRIHKFSASNLYFSKIFQKKWFQKFFGKIKYQYDARLLGGKRAIIMSNE
                     YDKVLPMDILPEYLFKAMITGNIEKMEALGGYEIAPEDVALCEFICTSKLPLQLIVRD
                     ALDTMKKELE"
     gene            7913..9124
                     /locus_tag="PLR72_01700"
     CDS             7913..9124
                     /locus_tag="PLR72_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOK35958.1"
                     /translation="MKNALRNFMDKVKPHFEKGGKFEKLHSVYDGFETFLFVPNTTAP
                     KYGSHIHDPNDTKRTMITVVIALIPALLFGMYNTGYQHYLAIGVNANFWITFLFGFLA
                     VLPYIIVSYVVGLGIEFTVAQIKGEEIQEGFLVTGFLIPLILPINTPLWMVAIATAFA
                     VIFAKEVFGGTGMNIFNVALVTRAFLFFAYPTHMTGDSVWVRTGSALGFGGGKAIDAF
                     SGATPLGQIATSTNSLPHITDTFGNTLSFWDGFIGLIPGSVGETSKIAILLGAILLLV
                     TGVASWKTMLSVFLGGAFMGIIFNLFGPDTASANFPWYCHLVFGGFAFGAVFMATDPV
                     TSARTEKGKWIYGFLIGCMAIIIRVLNPGYAEGMMLSILLMNIFAPLIDYYVVEANVK
                     QRNKRITKPVQ"
     gene            9172..9894
                     /gene="nqrC"
                     /locus_tag="PLR72_01705"
     CDS             9172..9894
                     /gene="nqrC"
                     /locus_tag="PLR72_01705"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOK35959.1"
                     /translation="MDTNSNSYTLIYATIVVVIVALMLAFVSGALKEKQNANIELDKK
                     RQILSSLQIDTKGKDVASLYDQYIVKGLVINYKGEILSESKKEAFNIDFAKELTKKLE
                     DRRLPLYIANVDGETKYILTLRGTGLWGPIWGYLALNDDKNTIYGVYFSHASETPGLG
                     ASIAELPFQKQFIGKHILNDKNEFVSIAVMKAGKKAEGKDQVDAISGGTITSKGVEAM
                     LLNCIGQYVQFLQQPTTGGMNQ"
     gene            9891..10523
                     /locus_tag="PLR72_01710"
     CDS             9891..10523
                     /locus_tag="PLR72_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOK35960.1"
                     /translation="MSKLFSRKSKQAFLWPLSKNNPIMVQILGICSALAVTAKLKPAL
                     VMGISVTIIVAFSNVIVSLIRDTIPNRIRIIIQLVVVAALVTIVSEILKAYVYDVSVQ
                     LSVYVGLIITNCILMGRLEAYAMSNKPWDSFLDGLGNGLGYAIILVIVSFFRELLGSG
                     TLFDIRIIPASFYEAGYMNNGLMLMPPMALILIACIVWIHRYHNKELQEK"
     gene            10548..11165
                     /gene="nqrE"
                     /locus_tag="PLR72_01715"
     CDS             10548..11165
                     /gene="nqrE"
                     /locus_tag="PLR72_01715"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOK35961.1"
                     /translation="MENVFNIFVKSIFVDNMIFAYFLGMCSYVAVSKNVKTSMGLGIA
                     VTFVLLITVPVNYLLENYVLKEGSLKWLSPELANLDLSYLSFILFIAVIAGIVQFVEM
                     VVEKFLPSLYNSLGIFLPLIAVNCAIMGASLFMQQRAYHNIGEATAYALGSGIGWWLA
                     IIGLAAIREKMEYSDVPAPLKGLGITFITVGLMGIAFMCFSGLKL"
     gene            11226..12491
                     /gene="nqrF"
                     /locus_tag="PLR72_01720"
     CDS             11226..12491
                     /gene="nqrF"
                     /locus_tag="PLR72_01720"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOK35962.1"
                     /translation="MLLAIDTVNLVASLVVFLVVILLLVIILLVAKQYLISSGTVTIT
                     INENKKLEVPAGGTLLNTLAMQQIFLPSACGGKGTCAQCKCQVFEGGGEILPTEISHF
                     TRKEIKEKWRLSCQVKVKENISIKIPPSILSVKKWECKVISNKNVATFIKELIVKLPE
                     GERLDFIPGSYIQIDIPKYNTIKFSDFDIDERFRSDWDKMKLWDLSCDNDEETMRAYS
                     MANYPAEGKDIVMLNVRITTPPIDKSTGTFMDVKPGIASTYIFNLRPGDKVKVSGPFG
                     EFHPIINSKREMLYIGGGAGMAPLRSHILHLLKTLQVRDRKISYWYGARSKNEIFYEE
                     DFRELEKEFPNFTFQIALSEPLPEDNWTGYVGFIHSVIYDNYLKNHPEPEEIEYYMCG
                     PGPMAKAVEKMLDDLGVPREMLMFDDFGA"
     gene            complement(12537..13196)
                     /locus_tag="PLR72_01725"
     CDS             complement(12537..13196)
                     /locus_tag="PLR72_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35963.1"
                     /translation="MYNESLFLSAISESYKNFVEYGPRSNKKLIPLHTFVGETLQNIW
                     GKDFELYFISESTKEFTVEGKYYPKAIDITVAQNGIPVFCFGVKFITSNYKQNSNNYF
                     ENMMGETANIQAKKDLPYAQLIILRHETPYYEKTVQKSGRKKATKIEIINEYYLQKYL
                     NLIFDSPQAHRPFAMGIILINVNEQTCNVEKVNLNSVFEADFAELFEKKLSITAEFKL
                     H"
     gene            complement(13455..13790)
                     /locus_tag="PLR72_01730"
     CDS             complement(13455..13790)
                     /locus_tag="PLR72_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOK35964.1"
                     /translation="MDWKEKLKEIKATLPQVEDQEKEDEKATAKNIQKEPLRIQLEKR
                     NNKVVTLVSEFQGSEEELKDLARLLKMKCSSGGTYSDNQILIQGDFRVKIAEILENLG
                     YKVKKINFK"
     gene            complement(13805..14341)
                     /locus_tag="PLR72_01735"
     CDS             complement(13805..14341)
                     /locus_tag="PLR72_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOK35965.1"
                     /translation="MRKLKVTEMNRLTVEEFKQQSKLPLVIVLDNVRSLHNVGSVFRT
                     ADAFRVEEIYLCGITRIPPHPELHKTALGAEDAVNWRYFQDTHRAIDDLKTKGYSIFS
                     IEQAEGSTLLQNLQLPKNGKFAVVLGNEVKGVQQTVIDLCDGCIEIPQFGTKHSLNVS
                     VTCGIIVWEFFKKIALSL"
     gene            complement(14401..15423)
                     /locus_tag="PLR72_01740"
     CDS             complement(14401..15423)
                     /locus_tag="PLR72_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOK35966.1"
                     /translation="MQKYLVIFLLAIFSFSCIEDPDIQPNTFDGNFKALWEIIDTRYC
                     YLDYKHINWDSVYYAYHLRVSYVKNEYELFDLMDKMLAELKDGHVNLYSPFNVSRYWN
                     WFTDYPKNFNDSLIYTDRYLGKNYRIAGGMHYEMINNGNIGYIYFGDFEKEFTDTNIA
                     YIFDYFSHCKGIIIDVRNNGGGYLSSAEQLASYFFKEKTLTGYISHKTGPGHSDFSEP
                     IKMYTTPHKKLRWLRPVVILTNRMSYSATNDFVNRMKLAPYALIVGDKTGGGGGLPLS
                     SEIPNGWMIRFSASPIFDSHMQHIEEGIEPDIQKDMSKRGAINGYDSIIEEAIRQINS
                     YFSKTY"
     gene            complement(15439..16290)
                     /locus_tag="PLR72_01745"
     CDS             complement(15439..16290)
                     /locus_tag="PLR72_01745"
                     /inference="COORDINATES: protein motif:HMM:NF023205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3316 domain-containing protein"
                     /protein_id="HOK35967.1"
                     /translation="MKRYVFPIFLVIVCLKVFSQDLSSPIYSLTTTNNLYGVTFIHLL
                     DNYLSPLTYDGIGFRYDYSSRKYLSSNNLKLSRENQFNINIAVTENPAKTSNISYLGM
                     DYGWGLHYHFRPAKNVQLLAGGLWNIEFGLKANSRNQNNPMNIDLATNLNFSGMTIYD
                     TRILKYPFRFQFTFSSPFLGLMFIPEQGESYYEIFELKNYSDILHFSSFHNKQGFTEE
                     LTIQVPLKHTTWKLAVRNDYLKYSANEMVFKRNDWAISLGYVFDIYKFAGRKNKLPAN
                     CISPDYK"
     gene            16567..18108
                     /locus_tag="PLR72_01750"
     CDS             16567..18108
                     /locus_tag="PLR72_01750"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOK35968.1"
                     /translation="MSQEEVFKKLIAHCKEYGFVFPSSEIYEGLSAVYDYGQNGVELK
                     NNIKRYWWESMTMLNENVVGLDAAIFMHPTTWKASGHVDAFNDPLIDNKDSKKRYRAD
                     VLIEDLIAKYDEKIEKEVEKAAKRFGKNFDEALFRSTNEQVKEHIAKREELHRRYLKA
                     MIENDLAELKAIIMDYDIVCPISGTKNWTDVRQFNLMFTTELGSNSDATMTIYLRPET
                     AQGIFVNFLNVQKTGRMKIPFGIAQIGKAFRNEIIARQFIFRMREFEQMEMQFFVRPG
                     EEMKWFEYWKNFRMKWHKALGFGDEKYRFHDHEKLAHYANAATDIEFEFPFGFKEIEG
                     IHSRTDFDLKNHEKYSGKSLKYFDIDLNESYIPYVIETSIGLDRTFLSIMAAAYTEEK
                     LENNESRVVLKFPPALAPIKVAIFPLIRRDGLPEIAHKIYEDLKLDFKCFYDEKDSIG
                     KRYRRQDAIGTPFCITVDHTTLEDNTVTIRYRDSMEQERVKIEALSSVISEKISMKNL
                     LKKIL"
     gene            complement(18174..18782)
                     /locus_tag="PLR72_01755"
     CDS             complement(18174..18782)
                     /locus_tag="PLR72_01755"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOK35969.1"
                     /translation="MLIKIYNTNPNSNEIRRVIEVLRQGGVIIFPTDTVYAFGCDIFN
                     KKGVEYIIRLKKKDLRRSQLSFVCHDLSEASEYARLDDRTFKLLKDNLPGPFTFILNG
                     SHKLPKLFKDKKTVGIRMPNNNIALEIVRELGNPMMTSSIFYHEDTKEYITDPELIEE
                     KYGKLVDLVIDGGEGGYIPSTVVDCTGDEPVIIRQGAGQLVY"
BASE COUNT         6343 a         2851 c         3127 g         6506 t
ORIGIN      
        1 attaaaatag aaatatttta tgtatttttg aaaaataaaa gtataaaaac tttattttct
       61 taaaaatcaa tcattaattt actatgttaa ttattgggat tgcaggaggt accggctctg
      121 gcaaaacaac agtagtacaa aaaattatgg aaagattgcc gaaaagtgaa gtagttgttt
      181 tgccacaaga tgcatattat cgtgatacca gttttttacc tttagaagaa agattggaaa
      241 ttaattttga tcatccggat tcaatagaat tcgagttatt gataaaacat ttaaaggatt
      301 tgaaagaagg aaaaagtatt gaaatgccca tttattctta tttaacctgt tcccgccaaa
      361 atgaaacaat taccattcat ccttgcgatg tggtaatggt agaagggatt ttggtacttt
      421 cacaacccga attaagaaaa ctcatgaatt tgaaagtttt tgtagatgcc gatgcagatg
      481 accgattgat aagagtgatt tatcgggata tcacggaacg tggtcggaca gttaataagg
      541 taatagaacg atatgaaaaa acagtaaaac ctatgcatga agaatttatt gagccaacta
      601 agcgctttgc cgatttgatt attcctcagg gaggaaataa caatattgct attgatattc
      661 tgacaaaata tattgaaaac aatttaaggt tgaaccataa ttctaattta gaaaactaaa
      721 aaaatactat ttctccatcg attttcaaca aattaaattt tccatagaaa ataattcatt
      781 ttataaatat taattgatat gcatttgaat gaattaaaaa agattatgtt tctggatata
      841 gaaacagtac cacaagtagc aaatttttct gatttgcccg atgagttggc tcatttatgg
      901 gaagaaaaat ttcatcattt gcgaaaacat ttaccagaaa aatataacga gaaaaccact
      961 gcttccgaag ctttcaatac aagtgctggt atctattcag agtttggaaa aattatctgt
     1021 attgcagtag gttttatcca ttttaaagaa gaagagatgt ttttacgaac caaatatttt
     1081 atcaatgagg atgaaaaaga acttttaatg gaattttcac aactgattga aaaatttggc
     1141 tcatcgaaag aaaaccgact ttgtggacac aacatcaagg aatttgatgt tccctatatt
     1201 tgtcgaagga tgctgatcaa taatttgcct ttaccatcga tatttcaact ttcaggcaag
     1261 aaaccctggg aaagtagcct aattgacaca atggaactct ggaaatttgg tgattataaa
     1321 aattttacgt ccctgaaatt acttacggcg gtttttaata tccctactcc gaaggacgat
     1381 attgatggaa gtgaagtagc tcaggtttat tataaagaaa agaatattca acgtattgcc
     1441 caatactgta ccaaagatgt aattgcaaca gcacaggttt tcttgcgatt aaatggcttt
     1501 ccgcctattt cagctgaaaa tattgaaaac ctctaatttg tttctatgaa aaatctttat
     1561 ctgattattt tcaatttatt tcttatactt acattcgttt cctgcaaaaa aaacgaaatt
     1621 gataaaagag ctcagataag caatagagat ctggaacaaa ttattctttc cgataccctt
     1681 aaagtcacca caatgtatgg ttctacctct tattttctct ttcgtgatga ggatatggga
     1741 tttgattatg aaatggcaga aaatttagca gattttctaa aagtgaagct gaaagtagag
     1801 cttgccaata gcgaacctca aatgattcaa cttttaaaag atggcaaagt agatatagtg
     1861 gcttataaca tccccgaaac aaaagaatta aaaaaagact ttcaatatgt atttcaacag
     1921 ccacgcaaca gtatggtgat tgttcaaaga atgggtcgca atgctctttc cagtgttgtt
     1981 gaattaaaag ataaaaaagt gtatgtaaaa aataatagtg tttttcatca acgtttgaaa
     2041 gatttgaata acgaaattgg cggaggaata caaattataa ctgttcctga ttcaataacc
     2101 aatgatgaat tgattgaaat ggttgccaat agcaaaatag attttacagt agcttatcga
     2161 gataaagcgt tacttcatca gtcttatttt agacgtcttg attgtcgctt aacaataggt
     2221 tttgaacaaa ataatggttg gttgatacga aaaaccagta aaaatctttt gacagctatc
     2281 aaagaatggg aaagtctaca aaagacgcaa gataaaaaaa atagattatt aactaaatat
     2341 tggaataaaa gtccttattt tgtcattcat aaaataaaaa taccaaaagg agcaattagt
     2401 ccttatgatg atttatttaa gaaatatgca cctaccattg gttgggattg gcgattattg
     2461 gccgctcttg cttttcatga atcgcgtttt gatagtgcag ctatttcacg agtaggagct
     2521 ctcggactga tgcagttaat gcctcaaact gctgcccaat ttggcttaga caaaagtgat
     2581 atttttaatc ctgaaaaaaa tattgaagcc tctattcaat acattaaaag tttaaacatg
     2641 gcttttagtc aggttgtcga caaaaatgaa cgtatcaaat ttattatagc aagttataat
     2701 tctggccctt ctcatatact tgatgctatg gctttagctc aaaaatatgg taaaaatcca
     2761 catatctggt ttgataatgt agaagtatat ttactggaaa aaagaaatcc agaatattac
     2821 aatgatcctg tggtaaaaaa gggttattat agaggaactg aaactgttcg atatgttgag
     2881 aatgtattag atacttatga aaagtatctt aaacgcaaat aaataaaatt ctcatttttt
     2941 tattttataa actttatctt aattttattg ctgtagagtc gaaataatta tatttttgta
     3001 ttaaataaaa acattagacc atgaatcctt tactttttaa tttaaatggt tgggagatac
     3061 cgattattct tttggtaatt cttcttttgt ttggtggaaa aaaaatccct gaattcatga
     3121 gtggcttggg gaaaggtatt cgcagcttta aaaaaggttt gaatgatatt gaagaagaaa
     3181 taaaagctga cccggatgag aagaaaccta atgataataa atcatcttct tcaaaataat
     3241 ttgatttgaa ttttctttct ctaagaaaat aataaaagcc aagcaaaatt tttttgacgc
     3301 ttgtggtatt cactattaaa tttcaggata aatagatgaa agaaaaagaa atgtccttct
     3361 gggaacattt agaagaattc cggtggactt tgattagaat tattatagct attgctatta
     3421 caaccatcat aggtttgatt tttattccta aaattttcga ttctgttatc ctggctccgt
     3481 gttcttcaaa tttcgttctt tacagatttt ttgaaaaatt aagtggttat tttccttttc
     3541 tattgaatta ttcctcttcc tcttttcatg ttaagatatt gaacatcaat atggcaacac
     3601 aattcatgac atatatgtct gcttcattgg cttttggatt tatattttca attccatatg
     3661 ttctttatga gttgtggaaa tttatcagcc cagcacttta tgaaaaggaa accaaaggag
     3721 taaaaaaagc ttttctattg ggaggagtga tgtttctgat aggatgttta ttgggctatt
     3781 ttattatttt cccattaata ttcagatttt taattacttt taaattgagc aacaacattg
     3841 aaaatatgat ttctttagat tcctatatgc gaaattttta taccctcatt ttgttaatgg
     3901 gcgttgtttt tgaacttccc cttttattct ggttattgtc aaacatcgga cttattaccc
     3961 gttccttttt taaaaaatat cgacgatatg ctattattgt ctcattggtg ctggctgctt
     4021 taattacacc ttctggcgat ccgttttctc ttttcgttgt ggcaattcca ctttattttt
     4081 tatgggaatt tagtgcttta ttggtgaaga aagataattt tgaacaagat atttcaactt
     4141 tgtaaagtat tatatttcta aaaatcatat attaactttt aaaaaccggt aatcatggaa
     4201 aatgacttta ttaaaaccgt cactcatacc attaaacatt ggtatattcc tttaattatt
     4261 ggaatcttgt ttattgtaat cggaattttt tgttttatat cgccaatggt ttcttatttt
     4321 actttttcat tgattttctc agtctctttt cttttttctg gtattttgga aattattttt
     4381 tctattacaa atcgaaaaga aactgataat tggggctgga cattggcttt tggaattatt
     4441 actttcataa ttggtctttt gctggtgctt aatccacgta ttacgatgct ttcattgcct
     4501 tatttcatag gctttcttat gctgtttcgc tcaatttttg ccatgagcct ggcaatagat
     4561 ttaaaaaatt attatgttct gaattgggga tatttattag ctattgctat tctgggaatt
     4621 atcttttcct ttattttgct gctcaatcct ttgtttggag gaattgcgat tactttattg
     4681 ttaggtttta cttttttagc aagtggttgc tatagtattt atctttcttt tgaattaaag
     4741 cggctgaaaa aatatgcaga taaaattcca gacgaattaa aagaaagata tgaaagcatt
     4801 aagcgagaaa ttcaagaaaa aatttgggaa taatatttaa aactactcct gccctaccta
     4861 ttcttacttc aaaatataac ttttagaagt aagggtattt gttaatcatt tatatggaat
     4921 atctttcaat tcgctaattc attactttta caccgataag tttatcataa cgtcctcccc
     4981 acggacgatg gtaaacataa atggtatatt cattgttggt ttgccaataa ctaccttcta
     5041 ttttttctac agaagctttt ttcccccctt ttggaagaaa ccaatactga taattataac
     5101 ctccttgctt gagtaaaata gtcttattgt aacgtccttc atcaaaattg tactgcattt
     5161 gcgatgtttc attcagtaaa ttataattat actctccacc aatgtaaact tgtccgtcaa
     5221 aataaggttg acccgtatat aatgaaaaat gaacttgcat ataatcagct tcagtattaa
     5281 tatcatcaaa tgcttcttga taatgaataa taaatttccc gttcaaatct ggctgagata
     5341 agtaattttt tgaagatgag ggaatatctt cttttaaata aacatcataa aatgttttat
     5401 taaagcgaat tgtatctaag ccttcaccaa caacataaat agatgacata tcaaaatggc
     5461 ggtattcatt acctccttca aatattagtg ctttttgatt aatgtaactt aatttttcag
     5521 tacttaaata agtaggcaaa atattagtta cttgattatc aattctgtta ttttgcctta
     5581 ttaccatttt aatttcagat tgagggtctt gtaaacgata gccatttaat ccaacttcaa
     5641 tatccaattg ctgaaaacgt ccacttaact caatatctgt atttgcgcga agtctcgcaa
     5701 taatatttac ttggggttcc accactgaaa aacaagctat cgcaacagga ttttccattt
     5761 tgttttcttc atagatcatt accatataat ttcctgatat tttaaaattc atttcctcgt
     5821 ttggcaaaat aaaacgataa tgtgtatatt gataagtagt attttgtgac aaagcataat
     5881 cgtaaatgtt gccagtagta aatccttcta aatattcatt agtcgataaa ttcgaaagtg
     5941 tccaatctgt gttacaatga aacactttat aagtaaaatt gtgaatatta tgcgataatt
     6001 catcaaactg aatttcaagg atatctttac tatttaattg tatgataggt aaactataat
     6061 tttgacctga taatccaatt tgtaaagttt taatagtggg atgcaaaatt tgagtatgat
     6121 aaattttttg tgaatgtaaa ctcatcacaa aaaataaagg gattaaaaat aaatttcgtt
     6181 tcatgagcta taaaaattaa aaaacgtgct aagtagaaat aaaacaaatt gtttgtacaa
     6241 tattacaaat atagccatgt tatttgaatt ttttaataaa acaataatat aaatggttta
     6301 tttttctatt aataactaaa ttattgataa ataataaata aattaattaa ttatatgatt
     6361 ttaattaatg aaataattct ttaataaaat gctgagtaac caataaaaaa tgtgtaattt
     6421 tgcaacgttt tttaaggtaa ggcaaaattt ccctaaaata gggattaaat ttaaatttag
     6481 aaattttatg gctaatctga taaaaactac gcggggtctt gatattccaa ttgaagggga
     6541 agctcaaaaa acattgggcg gcaccgttat ttcagatgta gtggcacttg taccggaata
     6601 ttatcatggc gtcgttccta aaatgttagt aaaagctggc gatgaagtta aagcaggttc
     6661 tcccattttt catgacaaga cttttgatac tatgaatttt gtttcaccag tgagtggtaa
     6721 agttttggct attaacagag gtgaacgtag aaagatatta agtatcgcta tcgaacgaga
     6781 tagcaaaatt caatatgttc cttttaaaat aaagccctta tctgaaacaa ctggtgaaga
     6841 ggtaaaagct cagttattga atgctggatt atggcctttc atcaagcaac gtccttatga
     6901 tattattgcc gacccaaata aatcacctaa agcaattttt atttcttgtt ttgattcctc
     6961 accgctggct ccagattaca attttatttt aaaaggtcaa ttcggtgatt ttcagactgg
     7021 tattgatgct ttaagcaaac ttaccaaagg aaagattcat cttggaataa aaccaacgaa
     7081 agattttaat gatttccgct ccgttcgaaa tgtagaaatt actgaatatg aaggacctca
     7141 tcctattggc aatgttggcg ttcaaattaa ccacgtcgat cctattaaca aaggagaaat
     7201 agtttggact attaatgttc agcatgtatt gtttattgga agatttttca ataaagggat
     7261 tgtcgattta agttatacac ttgctttaac cggtccttgt gtagttcaac cccaatatta
     7321 tcgtactctt gttggtgcta acatcgaacc ttttattaag gggaatgtat ataaaggtct
     7381 tattttacgc tatattacag gaaatgtgct cagtggaatt aaaatagaag aaaatgaatg
     7441 gttaaatccc tatgcttctc aaatttttgt tcttgaagaa ggaagtgatc aacatgaatt
     7501 tttaggttgg gcaatgccaa gaatccataa atttagtgcc agtaatttat atttttcaaa
     7561 aatatttcaa aaaaaatggt ttcaaaaatt ttttggcaaa attaaatatc aatatgatgc
     7621 ccgtttatta ggcggtaagc gtgccattat catgtccaat gaatatgata aagttttacc
     7681 tatggatatt ttgccagaat atctttttaa agccatgatt acaggaaata ttgaaaaaat
     7741 ggaagcactt ggaggatatg aaatcgctcc tgaagatgtg gctttatgtg aatttatttg
     7801 tacatctaaa ttgccacttc aacttattgt gcgtgatgct ttagatacaa tgaaaaaaga
     7861 acttgaataa cacaacaaca ttaaatttgc ctctaaagga aaaaatcgtg atatgaagaa
     7921 tgctttaaga aatttcatgg ataaagttaa acctcatttt gaaaaaggag gtaaatttga
     7981 aaaacttcat tctgtatatg atggttttga aaccttctta tttgttccca atactaccgc
     8041 tccaaagtat ggtagccaca tccatgatcc taatgataca aaacgtacca tgataacagt
     8101 ggtgatagct cttattccag ctcttttatt tgggatgtac aatactggtt atcaacacta
     8161 tttagctatt ggagtaaatg ccaatttttg gataactttt ttgtttggct ttttagcagt
     8221 tttaccctat ataattgttt cttatgtagt tggtttagga attgaattta cggtggctca
     8281 aataaaaggt gaagaaatcc aagaaggttt tttggtaaca ggatttctta ttcctttaat
     8341 tttaccaatc aatactccgt tgtggatggt agctattgcg acagcttttg ctgtaatttt
     8401 tgcaaaagaa gtttttggtg gaactggaat gaatattttt aatgtagctt tagttacacg
     8461 agctttttta ttctttgctt atccaactca catgacaggt gactcagtgt gggtacggac
     8521 aggaagtgcc ttaggttttg gtggcggtaa agcaattgat gctttttcag gtgctactcc
     8581 gctgggacaa attgctactt ccacaaatag cctccctcat attaccgata cttttggcaa
     8641 tacattaagt ttttgggatg gatttatcgg attaatccct ggctcggtag gagaaacttc
     8701 taaaattgcc attttgcttg gggcaatact tttgctcgtt actggtgtag caagttggaa
     8761 aactatgctt tcagtatttt taggcggtgc ttttatggga attattttta atttgttcgg
     8821 tcctgataca gcttctgcta attttccatg gtattgtcat ttagtatttg gcggttttgc
     8881 gtttggtgca gtatttatgg ctactgaccc agtaacttca gctcgaacag aaaaagggaa
     8941 atggatttat ggctttctga taggctgtat ggctatcatc attcgcgttt tgaatccagg
     9001 ttatgctgaa ggaatgatgc tttctatttt gctaatgaat atttttgctc cattaatcga
     9061 ttattatgta gttgaagcaa atgttaaaca acgcaacaaa agaataacaa agccagttca
     9121 ataaatttaa actgaaagct ctatttacaa ttattaaatt aaagataaag aatggatacc
     9181 aatagtaaca gttatacctt aatttatgct acaatagtag tggtaattgt agcattaatg
     9241 ttagcttttg tatcaggagc attgaaagaa aaacaaaatg caaatattga acttgacaaa
     9301 aaaagacaaa ttttaagttc cttacaaata gacacaaaag ggaaagatgt agcaagttta
     9361 tacgatcaat atattgtgaa aggtttggtt ataaactata aaggtgaaat tctttcagag
     9421 tccaaaaaag aagcttttaa tattgatttt gccaaagaat tgacaaaaaa attagaagac
     9481 agacgtttac ctctttatat tgccaacgtt gatggagaaa caaaatatat ccttactttg
     9541 cgagggacag gattgtgggg acctatctgg ggttatcttg ctttgaatga tgataaaaat
     9601 actatttatg gtgtttattt ttctcatgcc agtgaaaccc ctggtttagg tgcaagtatt
     9661 gccgaactgc cttttcagaa gcaatttatc ggtaaacata ttttaaatga taaaaatgaa
     9721 tttgtttcta ttgctgtaat gaaagctggt aaaaaagccg aaggaaaaga tcaggtggat
     9781 gctatttcgg ggggtactat taccagtaaa ggagtagaag ctatgttatt gaactgcata
     9841 ggacaatatg ttcaattttt acaacaacca accacaggag gaatgaatca atgagtaaat
     9901 tattttcaag aaaatcaaaa caagcctttt tatggccgct gagcaagaat aatcctatta
     9961 tggtacaaat attaggaatt tgttcagctc ttgcagtaac agccaaactg aaaccagctt
    10021 tggtaatggg aatttctgtt actattatag ttgctttttc taacgttatt gtttcattga
    10081 tacgtgatac cattcctaat cgaataagga ttattatcca attagttgtt gttgctgctt
    10141 tggtgacaat agttagcgaa attttaaaag cctatgtcta tgatgtaagt gttcaattat
    10201 cggtatatgt gggtttgatt attaccaatt gtatattaat gggacgattg gaagcttatg
    10261 caatgtccaa caaaccatgg gattcttttc tggacggttt aggtaatggt ttaggttatg
    10321 caattatatt ggtaattgta agctttttcc gtgaattatt aggttctggg actttgtttg
    10381 atattcgcat aattccagcc tcattttatg aggctggcta tatgaacaat gggttgatgt
    10441 taatgcctcc aatggcattg atattaattg catgtattgt ttggatacat cgttatcata
    10501 acaaagaatt acaagaaaaa taaaaatttc aaagataaat tttaacaatg gaaaatgtat
    10561 ttaatatatt cgttaaatcg atttttgtcg ataacatgat ttttgcatat tttttaggaa
    10621 tgtgttcgta tgtggctgtt tctaaaaatg taaaaacttc aatggggtta ggtatagctg
    10681 taactttcgt cctgcttatt actgttcctg tgaattatct tcttgaaaat tatgttttga
    10741 aagaaggatc cttgaaatgg ctaagtcctg aattagcaaa tcttgattta agttatttat
    10801 cttttatttt atttattgca gtaattgcag gaatagttca atttgttgaa atggtggttg
    10861 aaaaatttct tccttcgctt tataattcat taggaatttt cttgccttta atagcagtga
    10921 attgtgctat tatgggtgct tcccttttta tgcagcaacg tgcctatcat aatattggag
    10981 aagcaacagc ttatgctctt ggttcaggaa ttggctggtg gcttgctatt attgggttag
    11041 cagctataag agaaaaaatg gaatactccg atgttcctgc accgcttaaa ggacttggta
    11101 ttacattcat taccgttgga ttaatgggaa tagcatttat gtgcttttct ggattaaaac
    11161 tctgaccttt acttgtttaa aatcattaaa aaattctact ttaaaataaa taagcttata
    11221 tttatatgtt attagcgata gatacagtca atttggttgc cagtttagta gtttttcttg
    11281 ttgtaatttt acttttagtt attattttgc ttgttgcaaa acaatattta atttcttctg
    11341 gtaccgtaac catcactatt aatgagaata aaaaattaga agtgccagca ggaggaacgt
    11401 tattaaatac attagcgatg caacaaattt ttcttccttc cgcttgtgga ggtaaaggca
    11461 cttgtgctca atgtaagtgt caagttttcg aaggtggtgg tgaaatttta cccacagaaa
    11521 ttagtcattt tactcgaaaa gaaattaaag aaaaatggag attaagttgt caggtaaaag
    11581 taaaagaaaa tatttctatt aaaataccac cttctatttt aagtgtcaaa aaatgggagt
    11641 gtaaagtaat ttccaacaaa aatgtagcta cttttatcaa agaattgatt gtaaaattac
    11701 cagaaggcga aagattagat tttattcctg gaagttatat tcaaatagat attcctaaat
    11761 ataatacgat taaattttct gattttgata tagatgaacg tttccgttcc gattgggata
    11821 aaatgaagct ttgggattta tcgtgtgata atgatgaaga aacaatgcga gcttattcta
    11881 tggcaaatta tccagcagaa ggaaaagata ttgtaatgtt aaatgtacgt attactactc
    11941 caccaattga taaatctaca ggcacattta tggatgtaaa gccaggtatc gcttcaacat
    12001 atatttttaa cctcagacca ggtgataagg taaaggtgtc gggtccattt ggtgaatttc
    12061 atcccattat taattctaaa cgcgaaatgc tttatatcgg tggaggtgca ggaatggctc
    12121 ctctgcgctc tcatattttg catttactca aaacattgca ggtacgcgac aggaaaatat
    12181 cctactggta tggggctcgt tcgaaaaatg aaatttttta tgaagaagat ttccgtgaat
    12241 tagaaaaaga attcccaaac tttactttcc aaattgcact cagcgaacct ttgccagaag
    12301 ataattggac aggttatgta ggctttattc atagcgtaat atatgataat tatctgaaaa
    12361 atcaccctga accagaagaa attgaatatt acatgtgtgg accgggacct atggcaaaag
    12421 cagttgaaaa aatgttagat gatttagggg tacccagaga aatgctcatg ttcgatgatt
    12481 ttggggcata aagcaaataa tttttccaca gggcgttata acgtaaattc aagtaattaa
    12541 tgcaacttga attcagcagt tatagataat tttttttcaa ataattcagc aaaatctgct
    12601 tcgaaaacag agtttagatt tactttttca acattacaag tttgttcatt aacgttaatt
    12661 aaaatgatgc ccattgcaaa aggtctatga gcctgaggag aatcaaaaat taaattcaaa
    12721 tatttttgca aataatattc attgataatt tcaatttttg ttgccttttt tctaccactt
    12781 ttttgtaccg ttttttcata atagggcgtt tcatgtctta atatgattaa ttgagcatac
    12841 ggtaaatctt tctttgcttg aatattagcg gtttcaccca tcatattttc aaaataatta
    12901 ttagaatttt gtttgtagtt actcgtaata aatttaactc caaaacaaaa aactggtatt
    12961 ccattttgtg ctactgttat gtcaattgcc ttaggataat attttccttc aacagtaaat
    13021 tcttttgtgc tctcgcttat aaaatagagt tcaaaatctt ttccccaaat gttttgtaaa
    13081 gtttctccaa caaatgtgtg aagaggaatt aattttttat tgcttctggg tccatattca
    13141 acaaaattct tataggattc cgaaattgcg cttaaaaata aagattcatt atacataaat
    13201 ttgaatttaa ttgtaaggtt ctacaacttc attgatattt ttctcaatat aaaaagaaac
    13261 gtaacgaaaa taaaaaatga gaaaaaacaa cgcacaataa aaacaaatat acaaatttgt
    13321 ggttaaatta aagaaagttc tttgataatt ttttgattga taagaatatt ttaaatcttt
    13381 attctaacta aaaagtgaag aaaaaacttt ataaaataaa aattctcgtt taaggaataa
    13441 atttgtaaaa actattattt aaaattgatt tttttcactt tatatcccag attttccaaa
    13501 atttccgcaa tttttactct aaaatctccc tgaattaaaa tttgattatc agagtaagtg
    13561 ccaccactac tacatttcat tttcaataga cgagccagat ctttcaactc ttcttcactg
    13621 ccttgaaatt cactgactaa agtcaccact ttgttatttc gtttttctaa ctgaattcgc
    13681 aacggctctt tttgaatatt ttttgctgtc gccttttcat cttctttttc ttgatcttct
    13741 acttgtggca aagttgcttt tatttctttc agtttttctt tccaatccat atttgaaagt
    13801 atttttataa agatagtgct atttttttga aaaattccca aacaatgatg ccgcacgtaa
    13861 cgcttacatt caatgaatgt ttggtaccaa attgagggat ttcaatacat ccatcgcaca
    13921 ggtcgatcac tgtttgctgt acacctttta cttcattacc cagcactaca gcaaatttgc
    13981 catttttggg caattgtaaa ttttgcaata aggtgcttcc ttcagcttgt tcaatagaaa
    14041 aaatgctgta acctttcgtc tttaaatcgt caattgcccg atgcgtatcc tgaaaatacc
    14101 gccagtttac tgcatcttct gctcctaaag ctgttttatg gagttcagga tgcggaggta
    14161 tacgtgtaat gccacacaaa taaatttctt ccacacgaaa agcatcagcg gtgcgaaata
    14221 cagaaccaac attatgtaaa ctcctaacat tgtccagcac aatcaccagc ggaagtttag
    14281 attgttgctt gaattcttcc acagtgaggc gattcatttc ggttactttc agctttcgca
    14341 taattttttt acatcaaaaa catttcctaa aaataaatca aaatattatt tgttattttg
    14401 ctaataagtt tttgaaaagt aagaatttat ttgtcggatt gcttcttcaa tgatcgaatc
    14461 atagccattt attgcccctc gtttactcat atctttttga atgtcaggtt caatcccttc
    14521 ttcaatatgt tgcatgtggc tgtcaaaaat aggagaagca gaaaaacgaa tcatccagcc
    14581 atttgggatt tcgctcgaca aaggtaatcc accgccaccg ccagttttat cgccaacaat
    14641 gagagcataa ggtgccagtt tcattctgtt tacaaaatcg ttggtagcac tgtaagacat
    14701 ccgattggta agaattacaa cgggtcgcaa ccatcttaat tttttatgtg gagtagtata
    14761 catttttata ggttctgaaa aatcagaatg accgggacca gttttatgag aaatataacc
    14821 tgttaatgtt ttttccttaa aaaaataaga ggcaagctgc tcagcactac tcaagtaacc
    14881 acctccatta tttcttacat caataattat ccctttacaa tgcgaaaaat agtcaaaaat
    14941 ataggctata ttcgtatcag taaactcttt ttcaaaatcg ccaaaataaa tatagccaat
    15001 atttccattg ttaatcattt cataatgcat gcctccagca atccgatagt tttttcctaa
    15061 atagcgatcc gtgtaaatga gagaatcatt aaaatttttt ggataatcgg taaaccagtt
    15121 ccaatatcgg ctgacattga aaggagaata taaattcaca tgtccatctt tcaattcagc
    15181 aagcatttta tccatcaaat cgaacaattc atattcattt ttaacgtaag aaactctaag
    15241 atgataggca taataaacag aatcccaatt aatgtgctta taatccagat aacaatatcg
    15301 tgtatcaata atttcccata gtgccttgaa gttgccgtca aaagtattgg gctgtatatc
    15361 aggatcttca atacatgaaa aagagaaaat cgccaacaag aaaattacca gatatttttg
    15421 catcgagaat ctttcttttt atttgtaatc aggactaata caattggcag gcaacttatt
    15481 ttttctacca gcaaatttat aaatatcaaa tacatagccc agtgaaatag cccaatcgtt
    15541 acgtttaaaa accatttcat tagccgaata cttcaagtaa tcatttctga cggctaattt
    15601 ccacgttgta tgttttaaag ggacttgaat agttaattct tctgtaaaac cttgtttgtt
    15661 atggaaagaa ctaaaatgaa gaatatcgga ataatttttc aattcaaaaa tttcataata
    15721 agattctcct tgttctggga taaacataag acctaaaaaa ggagaagaaa aagtaaattg
    15781 gaaacgaaaa ggatatttca aaattcttgt atcgtaaatt gtcatgcctg aaaaattcaa
    15841 attcgtagct aaatctatat tcatgggatt attttggttt cgactgttag ctttcaagcc
    15901 gaactcaata ttccacaaac cgccagctaa caattgaaca ttctttgcag gtcgaaaatg
    15961 gtaatgcaaa ccccagccat aatccattcc taaataactg atattgcttg ttttagcagg
    16021 attttcggtg acggcaatat ttatattaaa ttgattctct cgtgatagct ttaggttgtt
    16081 ggaagaaagg tatttcctgc ttgaataatc ataacggaat cctatgccat cgtaagtcaa
    16141 tggagaaaga taattatcca gcaaatgtat aaaagtgact ccatacaaat tgttggtagt
    16201 agttaatgaa taaataggag atgacaaatc ctgagagaaa acttttaaac aaactatcac
    16261 taaaaaaatg gggaaaacat agcgtttcat ttagtcaaaa ttaatttttt tcctcataaa
    16321 tttttaaaaa tcaaaagtaa ataattcttt gtaaaaaaac gaaattattg aatataaact
    16381 ttactaaaaa atatcagaat aacttatttc aatttttaaa acctaaaaaa tcaacatatt
    16441 tcttaataga gctcccagaa aatgaaaaaa aactgtaatt ttgcaaaact ttttgtaact
    16501 tttttggaag tttcaaagaa acttgaaaat tacatcaatc ataaaaaatc gaaaatcaaa
    16561 tatatgattt cacaggaaga agtttttaaa aaactgatag ctcattgcaa agaatatggt
    16621 ttcgtctttc catccagcga aatttatgaa ggtttaagtg ccgtgtatga ttatggtcag
    16681 aatggagtcg aattaaaaaa taacattaaa agatattggt gggaaagcat gaccatgctg
    16741 aacgaaaatg tagtaggact cgatgccgcc atttttatgc atcctactac gtggaaagct
    16801 tccggtcatg ttgatgcttt taatgatcca ttaattgaca ataaagacag taaaaaacga
    16861 tacagagcag acgttctgat tgaagattta attgcaaaat atgatgaaaa gatagaaaag
    16921 gaagtggaaa aagcagcaaa gcggtttggc aaaaattttg acgaagcact ttttcgctct
    16981 actaatgaac aggtgaaaga acacattgcg aaacgtgaag aacttcatcg ccgttatcta
    17041 aaagcaatga tagaaaatga tctggcagag ctgaaagcaa tcatcatgga ttatgacatt
    17101 gtttgcccca tcagcggcac caaaaattgg acagatgtgc gacagttcaa tcttatgttt
    17161 actactgaat taggctcgaa ttctgatgcg acaatgacca tttatttgcg tcccgaaact
    17221 gctcaaggaa tttttgttaa ttttctgaat gtgcaaaaga caggaagaat gaaaattcct
    17281 tttggtatcg ctcaaattgg caaagctttt cgtaatgaaa tcattgcacg tcaatttatt
    17341 ttcagaatgc gagaatttga acaaatggaa atgcaatttt ttgttcgtcc tggtgaagaa
    17401 atgaaatggt ttgaatattg gaaaaacttc cgaatgaaat ggcacaaagc attgggattt
    17461 ggtgatgaaa aatatcgttt ccatgatcac gaaaaacttg ctcattatgc caatgccgct
    17521 actgatattg aatttgaatt tcctttcggc tttaaagaaa ttgaaggcat tcactcgcga
    17581 accgattttg acttgaaaaa tcatgaaaaa tattcaggga aaagtttgaa atattttgat
    17641 atagatctga atgaatctta tattccttac gttattgaaa cttccattgg acttgatcgg
    17701 acttttcttt ccattatggc agcagcttac acggaagaga aacttgaaaa caatgaaagt
    17761 cgagtagtac tgaaatttcc acctgcactt gctccgatta aagtagccat ttttccgtta
    17821 atcagaagag atggtcttcc tgaaatagct cacaaaattt atgaagactt aaaacttgat
    17881 tttaaatgtt tttatgacga aaaagactct atcggcaagc gctaccgtcg tcaggatgcc
    17941 attggtacac cgttttgcat tacagttgat catacaacac tggaagacaa tacggtaacg
    18001 attcgttatc gtgattcaat ggaacaggaa cgtgtaaaaa ttgaagctct ttccagtgtg
    18061 attagcgaaa agataagtat gaaaaatcta ttaaagaaaa tattataaaa gattttttaa
    18121 aagctcaatt taaccatcag cagggacggg cttgttcctg cttttttatt tttttaataa
    18181 acaagttgac cagcaccttg tctaatgata acaggttcat cgcctgtaca atcaaccact
    18241 gtagaaggaa tataaccgcc ttcgccacca tctatcacca gatcaactaa tttcccatat
    18301 ttctcttcga ttaattctgg atcagtgata tattccttag tatcttcgtg ataaaaaatg
    18361 gaactggtca tcataggatt tcccaattca cgaacaattt ccaatgcaat attattattg
    18421 ggcattctaa tccctactgt ttttttatct ttaaaaagtt tgggcaattt atgacttcca
    18481 ttcagaataa aagtaaaagg tcccggcaaa ttgtccttta aaagtttaaa agttctatca
    18541 tcgagtctgg catattcact tgcttcactt aaatcatgac aaacaaagga aagttgtgaa
    18601 cgtcgtaaat cttttttctt taatctgatg atatattcta ctcctttttt gttaaaaata
    18661 tcgcatccaa acgcataaac agtatcagtt ggaaaaataa taacaccacc ctgacgtaaa
    18721 acttctatta cacgacggat ttcattcgaa ttaggattgg tattgtaaat tttaatcaac
    18781 atttttttat ttttttctct ttaagaaaat ttcttttctt ttaatat
//
LOCUS       DAOSAS010000020        18557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_2085_length_18557_cov_13.544806, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000020 DAOSAS010000000
VERSION     DAOSAS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18557
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2085_length_18557_cov_13.544806"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..>1797
                     /locus_tag="PLR72_01760"
     CDS             44..>1797
                     /locus_tag="PLR72_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOK35970.1"
                     /translation="MEDSERIEVFGAREHNLKNIDVSIPRDALTVITGLSGSGKSSLA
                     FDTIFAEGQRRYMDTFSAYARNFIGNLQRPDVDQITGLSPVISIEQKTTNKNPRSTVG
                     TTTEIYDFFRLLFARASEAYSYVTGEKMVKYSEDQIVDLILKEYNGKKIILLAPVVRG
                     RKGHYKELFEQIRKKGYLHVRIDEKIQDVIPGLKVNRYKNHDIEIVIDRLEIEEKGRR
                     RLKESLQKAMQQGNGIVMILENENQMVRYFSKKLMCPTSGISYDDPDPHHFSFNSPHG
                     ACPYCKGLGFVNEIDLSKVIPNKSLSIYNGGIAPLGKYRNSTIFWQVEAILEKYGCNL
                     KMPIVDIPEEALDEILNGTEEPLYFKNKFAGTTNYFPEYDGIIKYLEMQQEESASATE
                     QKWAHQYSKSVICPECNGARLKKESLFFRIHDKNIAELSEMDISELYQWVNTVEEYLS
                     EKQRKIASEILKEIRNRLQFMLEVGLNYLSLNRSSESLSGGENQRIRLATQIGSQLVN
                     VLYILDEPSIGLHQRDNLQLIHSLKQLRDNGNSIIVVEHDKSIMLAADYLIDLGPGAG
                     RMGGEVVFAGEPAKMLEA"
     assembly_gap    1798..1897
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1898..2713
                     /locus_tag="PLR72_01765"
     CDS             <1898..2713
                     /locus_tag="PLR72_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOK35971.1"
                     /translation="DSIEGLEYVDKVVEVDQSPIGRTPRSNPATYTGVFTDIRNLFAT
                     LPAAKMRGYKPGRFSFNTPGGRCEVCHGNGYKTIEMNFLPDVYVPCESCNGKRYNKET
                     LEVRFKGKSIADVLDLTIDQAVDFFENQPSILYKLKTLQDVGLGYIKLGQPSTTLSGG
                     ESQRVKLSAELSKRDTGKTLYILDEPTTGLHFEDVRVLLGVLNRLVDKGNTVIIIEHN
                     MDVIKTADYIIDLGPAGGEAGGKIICTGTPQEVANHPKSETGKFLKLELKSLI"
     gene            2760..2960
                     /locus_tag="PLR72_01770"
     CDS             2760..2960
                     /locus_tag="PLR72_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35972.1"
                     /translation="MKLKSLLFALGVFVTYLVIVVLLRLYTKQIPENAVILNFFSKND
                     FFIGLLVTFFVTLAHEQKKRLK"
     gene            3018..4187
                     /locus_tag="PLR72_01775"
     CDS             3018..4187
                     /locus_tag="PLR72_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOK35973.1"
                     /translation="MNQQRYHLRGVSSSKEDVHDAIKNIDKGLYPKAFCKIIPDMLGN
                     DSDYCNIMHADGAGTKSSLAYVYWKETGDLSVWKGIAQDALIMNIDDLLCVGAVNNIL
                     VSSTICRNKNLIPGEVISTIINGTDEIIQQLCEMGINVYFTGGETADVGDLVRTIIVD
                     STVACRMKRADIIDNANIKTGDVIVGLSSSGQAIYEKEYNSGIGSNGLTSARHDVFVH
                     YLAEKYPESYDPAMPDDLVYTGSYSLTDKIESLQLDAGKLVLSPTRTYAPVVKKILEE
                     LHPYIHGMIHCTGGGQTKILHFIDQLRVIKNNLFPLPPLFQIIQKESGTDWKEMYKVY
                     NMGHRFEIYLPQEVAQNVIDIARTFHIDAQIIGYCEASEKKELIIESEFGKLKYK"
     gene            complement(4198..4779)
                     /locus_tag="PLR72_01780"
     CDS             complement(4198..4779)
                     /locus_tag="PLR72_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOK35974.1"
                     /translation="MNISLLQIASAFMVLFAIIDILGSIPIILGIKSKGESVSPAKTS
                     LVAFVILVVFLFAGEWVLKLFHVDIESFAAAGSLIIFIFSLEMILDVEIFRNKGPEGV
                     SSIVPIAFPLIAGPGSFTTLLSLRAEYEMINILIALTLNIIFVYFVLKSTDKVEKIIG
                     KGGIYILRKFFGIILLAIAVRLFATNIKSLLAY"
     gene            complement(4776..5573)
                     /locus_tag="PLR72_01785"
     CDS             complement(4776..5573)
                     /locus_tag="PLR72_01785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK35975.1"
                     /translation="MLNASIVLYHHAVDTIKPLVETLRGSNLISDIFLIDNSPSPHPD
                     FEKLPVTYIFTGKNLGYGAAHNIAIRKTLEQNVPYHLVLNPDVTFEPEILEKIVEFMD
                     KNTNIGLLMPKVFYPDGKIQYLCKLLPTPFDLIFRRFFPQNFFKKRKEHFEIRASGYN
                     KIMDVPYLSGCFMFLRMEALKQVGLFDERFFMYPEDLDLTRRIHQKYRTVYYPEVSIV
                     HQHVQSSYENLRMLWIHIINMIKYFNKWGWFCDKERKEVNQEIMKQL"
     gene            complement(5582..6694)
                     /locus_tag="PLR72_01790"
     CDS             complement(5582..6694)
                     /locus_tag="PLR72_01790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK35976.1"
                     /translation="MKKILHISKYYYPYYGGIEDVAHTIIKELKPYYEQRIICFNHER
                     GTIFNINGTDVLRIGTCCNISSQPVSLKYRHLLKQTIDTFQPDYIHLHLPNPLVATYL
                     LTLNLHGAKIVSHWHADILGQKSFYKLYKPFEREILKKSYKIIATSEPYMNSSEAIKP
                     YIDKTLILPNTIDEKKLHLFKGEEEEINKIKEKFNHKKIVFFVGRHVPYKGIDYLIES
                     EKFIKEDCIILIGGTGEQSASLKEMAKDHPRVKFIGRLSNNEVKYYLHAADVFAFPSI
                     NRSEAFGVALAEALYCGLPAVSFKIEGSGTMWVNQNNKTGLVVENKNVEEFGKAISTL
                     LGNENLRIQMSEAAKDWARQNFLPNQILPTIEKVYI"
     gene            complement(6697..7734)
                     /locus_tag="PLR72_01795"
     CDS             complement(6697..7734)
                     /locus_tag="PLR72_01795"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOK35977.1"
                     /translation="MPNKIAVDCRMLNKSGIGIYLKNLLYYWLQLPNIQWILIGRENE
                     LITLPLNENCHIIECNIPIFSLKELVNFPTEEINKCDLFYSPNFNVPGGINIPIYITI
                     HDVVFLDFPELVNPLGLWIRRYMLKRAFKLAYKIFTVSEFSKQRIIYHLGNGKEIIVA
                     YNGLRADLLNFTSENVSRIYDFDYFLFIGNIKKYKGLNILLEAMEGIDKKLVIVGTTQ
                     NLKTSDKIIIEKIRSNENIKFENYIKNDEILYSIIANAIALIQPSRYEGFGIPPLESL
                     FLGTPAIVSDIPVFREIYGKLPVIFFKDGDSNDLRLKLKKQFFPHIDTQVVKDAYSYQ
                     TTAFQILDQMR"
     gene            complement(7845..8933)
                     /locus_tag="PLR72_01800"
     CDS             complement(7845..8933)
                     /locus_tag="PLR72_01800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK35978.1"
                     /translation="MRILFVTTSKDILGGGELVSNRNYNSLLTIAGEKNVIKYSIKRN
                     QQDSIFFKLKTLFNEVINLNFNGLNKSQKRVIIGIIIKNQIDCIFFDTSSLGFFAYQL
                     KKKFPLIEVITYFHNVELSLAKDALKVNKNIFLFHRIIISYLSEKLICKYSDKIIVIC
                     ERDAQLILRYYKRKHDEIIPVSIKDRYTPIPKKIINKNCAQKTAIFVGSYFFPNIEAI
                     KWLINNIIPFVNIRLLLVGSNLDKLNYLVKDLSNIEIYSNVENIGYYYEQAHFALFPI
                     FSGSGMKVKTAEALMYGKYIMGTKETFVGYDYNPKIGYCGSTKKDFIDHINHFNPKSF
                     FNQYSRDLYLKKYSDNAVNELFKKLLER"
     gene            complement(8930..10111)
                     /locus_tag="PLR72_01805"
     CDS             complement(8930..10111)
                     /locus_tag="PLR72_01805"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HOK35979.1"
                     /translation="MKIFILKMPYSIPYILLICFFLLIATFQLGVQLDQKTRRYLNYI
                     VILTYILFFGFRGFIGWDWYNYYPFYKNIKIGENLLQNQNNISDYGFIFFAALIKYLF
                     KSYDSFILINTLINIILLNAIFNRYLDQKYHAFGYALFLAFNGLFLEVDLLRNVKALL
                     IFLIAIQYIEERKPLPYFALIILAFLFHWSAIFYAPLYFFLHKPISLKAFLIILVIGN
                     LIFIFQIEYLKPIIRAVSSLFPDTISTKINHYLDIKIYSRPYGFTLGFFERTIMSLFI
                     LLYYNKLVQIQTNILFINSFLIYICLYLFCSEVSIILSRVAINFVYSYWILIPIILQK
                     MDKGSKPIFYLFYCLLIILKINIMTNNIFYNYQSSLLSNRNSYTEQLEIFNKNYKSLL
                     K"
     gene            complement(10123..11073)
                     /locus_tag="PLR72_01810"
     CDS             complement(10123..11073)
                     /locus_tag="PLR72_01810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK35980.1"
                     /translation="MDVSIIIVNYNTKDLLFNCIKSIYKNTNHIDFEIIVVDNNSNDN
                     SDQMILNNFPEVRLIKLPKNVGFGKANNEGVKIAKGKYLFFLNSDTILLNNAIKVFFD
                     FMENHLPNLKIGAIGSLLVNKNYHIIHSFRHLPQIYKDIISDLKSNIINIMIGKERYF
                     RFKTWLKNSLRKKENNYFFNDNNFFEVGYVTGADLFMAKELFVELGGFDNNFFMYYEE
                     SDLQYRMKKKGFISVIINGPLIQHLEGASFRSNSAKQSITKKIYYDTSHFYYYKKNFN
                     SFIYNMYHIVYTLIQLIWIFDTSYSFKDRLKYIKFLISYK"
     gene            complement(11336..12592)
                     /locus_tag="PLR72_01815"
     CDS             complement(11336..12592)
                     /locus_tag="PLR72_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOK35981.1"
                     /translation="MRYKKEFWDTIYLVALQGLNYVAPLIVYPYLMIVLGAEKFGYIG
                     FSLAIIQYLMLIVDFGFNLSATKRIAIAQNNQSELNNIFSATLNAKLGLLCISFLFSL
                     FLAFGIPKFSIYSTTMMILFIMVIANAFSFVWLFQGLGKIRTISIVNIISKLLILPLT
                     FFFVKVPSDYNKAALIQSLVYVLSSSITCVILLNKKYITNYKIKTTKKQVLEEIKASY
                     PIFISNAATTIYTALFAIILGYFSTPVEVGKYTAVEKIMRSFCYLIFVPIVQSFYPKV
                     SYLSIIESKEAIKLVRKIILIIIILMSSIFCLLFFLSPYISIFLGKGYQGTEKIFKIM
                     SIIPLFIAVGGIFGQLGILALGKETDKKKFQRVYIIASIVAIVSIFILVPKFQSIGAS
                     FALLLTEFTVCILMSFYGKKVLFIRE"
     gene            complement(12589..12747)
                     /locus_tag="PLR72_01820"
     CDS             complement(12589..12747)
                     /locus_tag="PLR72_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HOK35982.1"
                     /translation="MIEGTYDEVMEVVKKAQQACYDAGAESIMTYIKIQSSKNEDVRI
                     ADKMEKYE"
     gene            complement(13085..13618)
                     /locus_tag="PLR72_01825"
     CDS             complement(13085..13618)
                     /locus_tag="PLR72_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOK35983.1"
                     /translation="MPVKIRLQRHGRKGYAYYHIVVADSRSPRDGKFIERIGSYNPNT
                     NPAIVALKFDRALYWLQVGAQPTDTARNLLSQEGVLLKKHLLGGVKKGAFDEAEAERR
                     FEAWKAFKEAKINMNINEVERKKQAEEKARLAAEAEQNKKKSEELAKKKAAEAAKAQK
                     ENPPEDESSQTNDNPTE"
     gene            complement(13720..14658)
                     /locus_tag="PLR72_01830"
     CDS             complement(13720..14658)
                     /locus_tag="PLR72_01830"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35984.1"
                     /translation="MEKLSYYVEKLLVRYDYVIVPDLGGFMVQNQSARILQDRIVPPL
                     LTLGFNPLLKDSDGLLAIAIAQEEGISYQSALEWIRKEVELIHFQLQSAKIVSLGKLG
                     FLKRNEIGKLVFVPAERVDFLPANFGMTDIQFFPKKSISSSQNKKVTITLPSASSMRN
                     VAATALVCVLLLVSPRIADTRRSFYTASFPLEMKRSSQTEKIIEKPTSVNSSSQVINT
                     ISELLTSEQEENTATTLKCPYHVIVASFYTEKAAEKFCESLHSIGFEKAHVISPQKNY
                     KVALQSFIEYNDAVEFLHHLHHSEPQFASAWVFIDK"
     gene            14779..15846
                     /gene="rfbB"
                     /locus_tag="PLR72_01835"
     CDS             14779..15846
                     /gene="rfbB"
                     /locus_tag="PLR72_01835"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOK35985.1"
                     /translation="MKRTILITGGAGFIGSHVVRLFVNKYPEYQIVNLDALTYAGNLE
                     NLKDVSNAPNYIFIKGDIRDRKLIPELFTTYQFDGVIHLAAESHVDRSISDPFAFLET
                     NIFGTVNLLDAAKKAWKNDMEGKLFYHISTDEVYGSLDENGYFYEGTAYDPHSPYSAS
                     KASSDHFVSAYYHTYGLPVIISHCSNNYGANQFPEKLIPLCINNIKNKKSIPVYGKGE
                     NVRDWLWVNDHAAAIDLIFHKGKVGEIYNISGNNEWKNIDLIKKLCEIMDRQLGRAEG
                     ESEKLITFIKDRPGHDLRYAIDSSKLQKELGWKPSVNFEEGLEKTVEWYLENEEWLNN
                     VINGDYLKYYEKQYQNQENEK"
     gene            16811..17932
                     /locus_tag="PLR72_01840"
     CDS             16811..17932
                     /locus_tag="PLR72_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOK35986.1"
                     /translation="MDLFNVYPLFNIEIVKGLGCRTYDKQGNEYLDFYGGHAVISVGH
                     SHPYFVKKLTEQIQKIIFYSNSVINPLQIKLADKLGKISGYEDYSLFLINSGAEANEN
                     ALKLASFHTGRKKVIAFSKSFHGRTSAAVSVTDNPSIVAPINENFEVEFFPLNDTVAV
                     KKSLRKRDVCAVIIEGIQGVGGIQIPNADFLQVLSAECKATGTILILDEVQSGYGRTG
                     KFFAHQYAGIRPDLITVAKGMGNGFPIGGVIISPMFKPIYGMLGTTFGGNHLACTAAL
                     AVLDIIEEEHLMENAKKIGDYLLDELQKFPRIKEVRGVGLMIGIEFEEPVSALRTKLL
                     YDKKIFTGVSGFNIIRLLPPLCLSKEEADLFLERFYELL"
     gene            17941..>18557
                     /locus_tag="PLR72_01845"
     CDS             17941..>18557
                     /locus_tag="PLR72_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35987.1"
                     /translation="MKTHFLLLIDFFIFLSLLNLSAQETVHDKLILKSGGIYVGEIIL
                     KNNEVVILQTTDGTRYQFPFSDIKEISKSSDIFPTKNISSNNDTLSFPFCAFLEFTGG
                     VAQAKNKFDLSPNSQITLIFGKKQLFNKGFFIGGAIGYNNTSLEQNNSLNFLPIFIRL
                     EDIPIKKRLSPYMTLDAGYAFALASDYSGGTMTKISGGITYKLTY"
BASE COUNT         6582 a         2755 c         2790 g         6330 t
ORIGIN      
        1 aaaaattatt gccttgatta tcaataaaga aaatataaaa attatagaag actcagaaag
       61 gattgaagta tttggcgcca gagagcataa tttaaaaaat attgatgtct ctattcctcg
      121 agatgcttta acagttatta caggactgag tgggagtgga aagtcttcct tagctttcga
      181 tactatattc gctgaaggac aacgaagata catggatact ttttctgctt atgctcgtaa
      241 ttttatagga aatttacaac gtcctgatgt tgaccaaatt acagggttga gccctgttat
      301 ttctatcgaa caaaaaacaa caaataaaaa tcctcgttcc actgttggta ctactacaga
      361 gatttacgat ttctttcgat tgttgtttgc cagagcaagt gaagcctatt cttatgtaac
      421 aggcgaaaaa atggttaagt attctgaaga tcaaatcgtt gatttgattt taaaagaata
      481 caatggtaaa aaaattattc ttttagcacc agttgttcga ggacgaaaag gacattataa
      541 agaattattt gagcaaatcc gtaaaaaagg atatttacat gtaagaattg atgaaaaaat
      601 tcaagatgta attcctggct taaaagtaaa tcgttacaaa aatcatgata tagaaattgt
      661 cattgatcgg ctggaaattg aagaaaaagg acgaagacgt ctgaaagagt cattgcaaaa
      721 agccatgcaa caagggaatg gaattgttat gattctggaa aatgaaaacc aaatggtacg
      781 ttatttcagc aaaaaattaa tgtgcccaac cagtggcatt tcatatgacg atcctgatcc
      841 gcatcatttt tcctttaatt cacctcacgg tgcttgccct tattgtaaag gattaggatt
      901 tgtaaatgaa attgatctca gtaaagttat acccaataaa tcgcttagta tttacaatgg
      961 tggaattgct ccgcttggga aatacagaaa ctctactatt ttctggcaag tcgaagccat
     1021 actcgaaaaa tacggttgta atttaaagat gcccattgtt gatattcctg aagaagctct
     1081 tgatgaaatt ttaaatggaa cagaagaacc tctttatttt aaaaataaat ttgcaggaac
     1141 taccaattat tttcctgaat atgatggcat tattaaatac cttgaaatgc aacaagaaga
     1201 aagtgcttct gcaacggaac aaaaatgggc tcatcaatat tcaaaatcag taatttgccc
     1261 agaatgcaat ggagcgagat taaaaaaaga atcgctgttt ttcagaatcc atgataaaaa
     1321 tattgctgaa ttgtctgaaa tggatatttc agaattgtac caatgggtaa atacagtcga
     1381 agagtatctt tctgaaaaac aaagaaaaat agcttctgaa attttaaagg aaatccgtaa
     1441 tcgtttgcaa ttcatgctgg aggtcggtct taattacctt tcccttaatc gtagttcaga
     1501 atctttgtca ggtggtgaaa atcaacgtat tcgtttagca acgcaaattg gttctcaatt
     1561 ggttaatgta ctttatattc tggatgagcc cagcattgga ttgcatcaac gcgataattt
     1621 acagcttatc cactcgttga aacaacttcg agacaatgga aattcaatta ttgttgttga
     1681 gcacgataaa agcattatgc ttgcggctga ttatcttatt gatttaggtc caggagcagg
     1741 acgaatgggt ggtgaagttg tttttgcagg tgaaccagct aaaatgttag aagctgannn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngac agtattgaag gattggaata
     1921 tgttgataaa gtcgtggaag tagatcaatc acctattggg agaacgccgc gttcaaatcc
     1981 agctacttat acaggggttt ttacagatat tcgaaatttg tttgctacat tgcctgctgc
     2041 taaaatgaga ggttacaaac ctggcagatt ttcatttaat acacctggtg gaagatgtga
     2101 agtttgtcat ggtaatggtt ataaaacgat agaaatgaat tttttacctg acgtttatgt
     2161 cccctgcgaa agctgcaatg ggaaacgtta caataaggaa acccttgaag ttcgttttaa
     2221 gggaaaatca atagctgatg tgctggactt aactattgat caggcggtag atttttttga
     2281 aaatcaacct tctattttat acaaacttaa aactttacag gatgtaggat taggatatat
     2341 taaactgggc cagcccagca ctacactttc tggcggcgaa agtcaaagag taaaattgag
     2401 tgccgaactt tcaaaacgag atacaggaaa aactttatat attttggatg aacctactac
     2461 cggtttacat tttgaagatg ttagagtatt actgggagtt ttgaatagac tggttgataa
     2521 aggaaacacc gttattatta ttgaacacaa catggatgta attaaaacag ccgattatat
     2581 cattgattta ggaccagcgg gaggagaagc tggcggaaaa attatttgta caggtactcc
     2641 tcaggaagtg gcaaaccatc ccaaaagtga aacaggtaaa tttctgaaat tggaattgaa
     2701 aagcttaatt tagttatttt tattatcttt ggcttcattc aaattaggaa atttttatta
     2761 tgaaactaaa atctttgtta tttgccttag gagtatttgt tacttatttg gtaatagttg
     2821 ttttattacg tttatataca aaacaaatcc ctgagaatgc ggttattttg aatttctttt
     2881 caaaaaatga tttttttatc ggcttgctgg tgaccttttt tgtaacgctc gctcatgaac
     2941 aaaagaaaag attgaaataa ttaattagct ttcaattatt taaaaataat ttcaaaatat
     3001 tttttaataa attttacatg aaccaacaac gataccattt aagaggcgtt tcttcctcaa
     3061 aagaagatgt gcacgatgct ataaaaaata ttgacaaggg cttgtatcca aaagcttttt
     3121 gtaaaattat tcctgatatg ctggggaatg attccgatta ctgtaatatt atgcatgctg
     3181 atggggctgg aactaaatcc tctttagcgt atgtttattg gaaagaaact ggtgatttgt
     3241 ccgtatggaa aggaatagct caggatgctt tgattatgaa tattgatgat ttgttgtgcg
     3301 taggagcagt aaataatatt ctggtttcat caacgatctg tagaaataaa aatctgattc
     3361 ctggcgaagt tatttcaacc ataatcaatg gaacagatga aattattcag cagttgtgcg
     3421 aaatgggaat aaatgtttat ttcactggtg gagaaacggc tgatgtaggt gatttggttc
     3481 gcactattat tgtggatagt accgttgctt gccggatgaa aagagctgat attattgata
     3541 atgccaatat aaaaacaggc gatgtgattg tgggactttc ttcaagtgga caggcaattt
     3601 acgaaaagga atacaacagt ggcataggca gcaatggctt aacatcagcc cgccacgatg
     3661 tgttcgttca ttatcttgca gaaaaatacc cagaaagtta tgaccccgct atgccagatg
     3721 atttggttta tactggctct tattcattaa cagataaaat tgaatcttta caattggatg
     3781 cgggtaagtt ggtactttct cccactcgga cctatgctcc tgtggtaaaa aaaattttag
     3841 aagaactcca tccttacatt catggaatga ttcattgtac aggaggagga caaactaaaa
     3901 ttttacattt cattgatcaa ctcagagtga ttaaaaataa tttgttcccc ttacctccac
     3961 tttttcaaat tattcaaaaa gaatcaggaa cagactggaa agaaatgtat aaagtttata
     4021 atatgggaca tcgatttgaa atatatctac cgcaggaagt tgcacaaaat gtaattgaca
     4081 ttgcaagaac ttttcatatt gatgcacaaa taattggata ttgtgaagct tctgaaaaaa
     4141 aagaattaat tattgaaagc gaattcggaa agcttaagta taaatgagac caacaaatta
     4201 ataagccagt aacgatttaa tatttgttgc aaatagtcta acagctatcg ctaataaaat
     4261 gattccaaaa aatttacgta aaatataaat accccctttg ccaattattt tttccacttt
     4321 atcagttgac tttaacacaa aataaacaaa aataatattt aaagtgaggg caatgaggat
     4381 attgatcatt tcatattcag cacgtaaaga aagtaaagtg gtaaacgagc caggaccagc
     4441 aattaaagga aaagcaatag gaacaatgga ggagacgccc tcaggacctt tatttcgaaa
     4501 aatttcaaca tccagaatca tttccagtga aaagataaaa ataatcaagg aaccggctgc
     4561 agcaaatgat tcaatatcaa catgaaaaag ttttaaaacc cattccccag caaacaaaaa
     4621 aacaaccaaa ataacaaatg ctactaaact ggttttagca ggcgaaacac tttctccttt
     4681 actttttata cccaaaataa ttggaatgga tcccaatata tcaataatgg caaaaagtac
     4741 catgaaggca cttgctatct gcaataaact aatattcata gctgtttcat aatttcctgg
     4801 ttcacttctt ttctctcttt atcgcaaaac caaccccatt tattgaaata ttttatcatg
     4861 ttaataatgt gaatccacaa cattcgtaaa ttttcataag aactttgaac atgttgatga
     4921 acaatgctca cttcaggata ataaaccgtt cgatattttt gatggattct ccgtgtcaaa
     4981 tcaagatctt caggatacat aaaaaatcgt tcatcaaata aaccaacttg ttttagagct
     5041 tccattctta aaaacataaa gcaacctgac agataaggta cgtccattat tttattatat
     5101 cctgacgcac gtatctcaaa atgttccttc ctttttttaa agaaattttg aggaaaaaat
     5161 cttctgaaaa tcaaatcaaa aggagttgga agcaatttac aaagatattg aatttttcca
     5221 tctggataaa atacttttgg cattaaaagt ccaatatttg tatttttatc cataaattcg
     5281 acaatttttt ccagaatttc aggctcaaaa gttacatcag gattgagcac cagatgataa
     5341 ggaacatttt gttccagggt tttcctgata gcaatattat gggcagcccc ataacccaga
     5401 tttttccctg taaaaatgta agtaacggga agtttttcaa aatcaggatg aggcgaggga
     5461 gaattgtcga taaggaaaat atcggaaata agattagacc ctcgaagcgt ctcaaccaaa
     5521 ggttttatcg tgtctacggc atgatgatat agaacaatgg aagcattcag catgaaaagc
     5581 gttaaatata aactttctca attgtaggca atatttgatt tggtaaaaaa ttttgtcgag
     5641 cccaatcttt cgcagcttca gacatttgaa tccttaaatt ctcattgcca agtaatgtgg
     5701 aaatagcttt gccaaattct tcaacatttt tattttctac gactaagcct gttttattgt
     5761 tctggttgac ccacattgtg cccgacccct caattttaaa agaaacggca ggtaaaccac
     5821 aatataatgc ttctgctaaa gctacaccaa atgcttcact tcggtttatt gatggaaagg
     5881 caaaaacatc tgctgcatgt aagtaatatt tcacttcatt atttgaaagc cttcctataa
     5941 atttaactct tggatgatct ttcgccatct cttttaaact tgcactttgt tcaccagtcc
     6001 cgccaatgag aatgatacaa tcctctttta tgaacttttc cgattcaatc aagtagtcaa
     6061 ttcctttata aggcacatgc cttccaacaa aaaagacaat ctttttatga ttaaattttt
     6121 cttttatttt attaatttct tcttcttcac ctttaaacaa atgcaatttt ttttcatcta
     6181 ttgtatttgg taatattaat gttttatcta tataaggttt tatcgcttca gaagaattca
     6241 tgtatggttc gcttgttgca ataattttgt acgatttttt tagaatttct ctttcaaaag
     6301 gtttatataa tttataaaaa gatttttggc ctaaaatgtc ggcatgccaa tgggaaacaa
     6361 tttttgctcc gtgtaaatta agagtaagca gataggttgc aactaatgga ttaggtaaat
     6421 gtaaatgtat ataatcaggc tgaaaagtat caatggtttg ttttaataaa tgcctatatt
     6481 tcaaagaaac cggttgagaa gatatgttac agcaggtgcc aattctcagc acatcagtcc
     6541 cgtttatatt aaaaattgta ccccgttcat gattgaaaca aataatgcgt tgttcataat
     6601 aaggcttcaa ttctttaatt atggtgtgag caacatcttc gatgccacca taatagggat
     6661 aataatactt agaaatgtgt aaaatttttt tcataactat ctcatttgat ccaatatctg
     6721 aaaagctgtt gtttggtaac tataagcgtc tttaacaact tgagtatcta tgtgagggaa
     6781 aaactgtttt tttaatttta accttaaatc atttgaatcg ccatctttaa aaaagatcac
     6841 tggcagcttc ccataaattt ctctaaatac aggaatatct gatacaattg caggcgtacc
     6901 taaaaacaaa gattccaatg gtggaattcc aaagccttca tatcgagacg gctgaattaa
     6961 agcaattgca ttagctataa ttgaatataa aatttcatca tttttaatat aattttcaaa
     7021 tttaatgttt tcatttgaac gaatcttttc aataattatt ttatcggacg tttttaaatt
     7081 ttgagttgtc ccaacaatga ctaatttttt atcaatgcct tccatagctt ccaacaatat
     7141 atttaatcct ttatactttt ttatatttcc aatgaataag aaatagtcaa aatcatatat
     7201 cctggaaacg ttttcagaag taaaattcaa taaatcagct cttaagccat tgtaagcgac
     7261 gataatttct ttcccattgc ctaaatgata aataattcgt tgcttagaaa attcagaaac
     7321 agtaaaaatt ttataagcta atttaaaagc acgttttaac atatatctac gaatccacaa
     7381 accgagagga ttaactaatt ccgggaaatc aagaaatact acatcatgga tagtaatata
     7441 aataggaata ttaatacctc ccgggacatt aaaattaggt gagtaaaata aatcacattt
     7501 gttaatttct tctgtaggaa aatttaccaa ttctttaagg gaaaaaatag gtatattgca
     7561 ttcaattata tgacaatttt catttaatgg gagagtaatt agttcgtttt ctcttcctat
     7621 taaaatccat tgaatattag gcaattgtaa ccaataatat aataaatttt taaggtatat
     7681 gcctatgcca cttttattaa gcattcgaca atctaccgct attttatttg gcattttatc
     7741 aaaagaattc atttgttaaa aattaaagtc aatctgttac cagaattaaa tttctttgct
     7801 tcttactaat agctttgaac taaaatcgta attgatttct tattttacct ttctaataat
     7861 tttttaaaca attcatttac agcattatca gaatattttt ttaaatataa atctcgagaa
     7921 tattgattaa aaaaactttt aggattaaaa tgatttatat ggtcaataaa atctttcttg
     7981 gttgagccac aataaccaat tttgggatta taatcatatc ctacaaatgt ttccttggtt
     8041 cccattatgt atttcccata cattaaagct tccgcagttt taaccttcat tcctgaacct
     8101 gaaaatatag gaaataaggc aaaatgagct tgctcataat agtaaccaat attttctaca
     8161 ttactataaa tttctatatt gcttaaatct tttacaagat aatttaattt atccaaattt
     8221 gaacctacta aaagtaatct aatatttaca aatggtatta tattatttat tagccattta
     8281 attgcttcta tattggggaa aaaatagcta ccaacaaaaa tagctgtttt ttgtgcacaa
     8341 tttttattga taattttttt aggaatagga gtataacgat ctttaatgct tacgggtata
     8401 atttcatcgt gctttcgctt ataatatcgt aatattaatt gggcatctct ttcgcaaatt
     8461 acaattattt tatcagaata tttacaaatc aatttttcac ttaaatatga aataattatt
     8521 ctatgaaaga gaaaaatatt cttgttaact ttaagagcgt cttttgcaag agataattct
     8581 acattatgga aataagttat cacttcaatt aaagggaatt tttttttcaa ttgataagca
     8641 aaaaaaccta aggatgaggt atcaaaaaaa atgcaatcaa tttgattttt tataatgatc
     8701 ccgattatta ctctcttttg tgacttattt aatccattaa aatttaaatt aattacttca
     8761 ttgaataaag tttttaattt gaaaaaaatt gaatcttgct ggtttctttt tattgaatat
     8821 ttaataacat ttttttcccc cgcaatagta agtaatgaat tataattcct atttgaaaca
     8881 agttcgccac cacctaatat atcttttgat gtagttacaa ataatattct cattttagca
     8941 aacttttata gttcttatta aaaatttcaa gttgttcggt ataagaattc ctattcgaaa
     9001 gaagagaaga ttgataatta tagaaaatat tatttgtcat tatattaatt tttaaaatta
     9061 tcaataaaca ataaaacaaa taaaaaatag gttttgaacc tttatccatt ttttgaagaa
     9121 taattggaat caatatccaa tatgaatata caaaatttat tgcgacacga cttaaaatga
     9181 ttgaaacctc tgaacaaaaa agataaagac atatgtatat taaaaaacta ttaataaata
     9241 aaatatttgt ttgaatttgt actaacttat tgtaatataa taatataaat aaagacatta
     9301 tcgtacgttc aaaaaaaccc agtgtaaaac catatggtct tgaatagatt ttaatgtcta
     9361 aatagtgatt tatttttgtt gaaatagtat cggggaagag agaagatact gctctaataa
     9421 ttggttttag atattctatt tgaaatataa aaattaaatt cccgattacc aaaataatta
     9481 aaaaagcttt taatgaaatg ggtttatgca aaaaaaagta taaaggagcg taaaaaatcg
     9541 cactccaatg aaacaaaaat gcaagaatta ttaaagcaaa ataaggaagt ggttttcttt
     9601 cttcaatata ttgtatagct attaaaaata ttagtaacgc tttcacattc cttaacaaat
     9661 ctacttcaag aaacaatcca ttaaaagcta gaaataaagc atacccaaat gcatggtatt
     9721 tttgatctaa ataacgatta aaaatagcat tcaataggat gatatttatt aaggtattga
     9781 tgagaataaa agaatcataa ctcttaaata aatactttat taatgcagca aaaaaaataa
     9841 aaccataatc agaaatatta ttttgatttt gaagtaaatt ttctccaatt ttaatatttt
     9901 tataaaaagg ataataatta taccaatccc acccaataaa acctcgaaat ccaaaaaata
     9961 atatataagt aagtataacg atatagttca aatatcttcg tgttttttgg tctaattgca
    10021 cacctaactg aaaagtagca attaacaaaa aaaaacaaat taataaaata tatggaatgg
    10081 aataaggcat ttttaaaata aaaattttca agaacgttta aatcatttat aacttataag
    10141 gaacttaata tattttaacc tgtctttaaa gctatatgat gtgtcgaaaa tccaaataag
    10201 ttgaataagt gtatagacta tatgatacat attatatata aaactgttga aatttttttt
    10261 gtaataataa aaatgacttg tgtcatagta aatttttttt gtgatagatt gttttgcact
    10321 gttgcttcta aaagaagctc cttccaaatg ttgtatcaaa ggtccattaa taattacact
    10381 aataaatcct ttttttttca ttctatattg aagatcggac tcttcataat acataaaaaa
    10441 attattatca aaaccaccta attcaacaaa taattctttt gccataaata agtctgcacc
    10501 cgtaacatat cctacttcaa aaaaattatt atcattaaaa aaataattat tctctttttt
    10561 tcttaatgaa ttttttagcc atgttttaaa cctaaaatat ctttcttttc ctatcattat
    10621 attaataatg ttagacttta aatctgatat aatatcttta tatatttgag gtaaatgtct
    10681 gaacgaatgt ataatatgat aattcttatt tactaataac gaaccaatgg caccaatttt
    10741 taaattgggt aaatgattct ccataaaatc aaagaaaact ttaattgcgt tatttaaaag
    10801 gatagtatca ctgttaagga aaaaaagata tttccctttt gcaattttta ctccttcatt
    10861 attagcttta ccaaaaccta catttttagg taattttatt aaccgaactt ctggaaaatt
    10921 gttcaaaatc atttgatctg aattatcatt cgaattattg tcaactacaa taatctcaaa
    10981 atcaatatga tttgtatttt tatatattga ttttatacaa ttaaataata aatccttggt
    11041 attataattt acaataatta ttgacacatc catttatggt agctattttc ttgaaaaaga
    11101 tttatcttgt attttgaaaa aaattaaaaa taaaattcaa attagttgct tttgaagact
    11161 aaatttaaat agagcaaaaa aacatataag aatataagaa tataaaaata aaaaaagcct
    11221 tctcaaaaaa taattaacat taaaagttat attcactact tgaattcaat atttatgatt
    11281 tacctaatta agtaaatttg ttgtcagatt acttcaagag aaaaatacaa aaaatttact
    11341 cccgaataaa taaaactttt ttcccataaa aagacatcaa aatacaaaca gtaaattcag
    11401 tcaataataa tgcaaaggaa gctcctattg actgaaattt tggcaccaga ataaatattg
    11461 agacaatagc tactatactc gctataatat aaactctttg gaactttttt ttatctgttt
    11521 ccttacctaa tgctaaaatt cccaattgac caaatatacc gccaaccgca atgaataaag
    11581 gaataattga catgatttta aaaatttttt cagttccttg atatcccttt cctaaaaaaa
    11641 ttgatatata aggagaaaga aaaaacaata aacaaaatat actactcatg agtattatta
    11701 taattaaaat tattttccgc actaatttta ttgcctcttt tgattctatt attgaaagat
    11761 aacttacctt tggataaaat gattgaacaa tgggaacaaa aatcaaataa caaaaactac
    11821 gcataatctt ttctactgca gtatattttc caacttctac tggtgttgaa aaatatccta
    11881 aaataattgc aaataatgct gtataaatag tagttgctgc atttgaaata aaaataggat
    11941 aactggcttt tatttcttct agaacttgct tttttgttgt tttaatttta taattagtaa
    12001 tatatttttt gttcaataaa atgacgcaag taatactact acttaaaaca taaactaaag
    12061 attgaattaa tgcagcctta ttataatcag aaggaacttt aacgaaaaag aatgttaagg
    12121 gtaatattaa aagctttgat ataatattaa caattgaaat tgttctaatc tttccaagtc
    12181 cttgaaaaag ccatacaaat gaaaaagcat ttgcaataac cataatgaat agtatcatca
    12241 tagttgttga atatattgaa aatttaggga ttccaaatgc taaaaataat gagaataaaa
    12301 aacttataca aagtaagcct aattttgcat ttaaagttgc tgaaaaaata ttattaagtt
    12361 ctgattgatt attctgagcg atagctatcc ttttcgtagc acttaaatta aatccaaaat
    12421 ctacaataag cattaaatat tgaattattg ccaaagaaaa tccaatatag ccaaattttt
    12481 cagctcctaa aacaatcatc aaataaggat agactattaa aggtgcaaca tagttaagtc
    12541 cttgcaatgc aactaaataa atagtatccc aaaactcttt tttatatctc attcgtattt
    12601 ttccatttta tctgcgattc ttacgtcttc atttttggaa ctttgaattt tgatataggt
    12661 cattatactt tctgcaccag catcatagca agcttgctgc gctttcttta ctacttccat
    12721 cacttcgtca taagttcctt ctatcaccgt ctcaaaagga gttactttgt attttagacc
    12781 tcaaactgcg ataacttcta ttgctttatc aactaaattg taagggtgaa caatttttga
    12841 agtaggaaga acttgaagtg caatgtttac aattttattc attttaacaa gtaattggaa
    12901 agaaacttag caattaatcg aaacgtatca actttttaaa aattgtgtaa tttttataac
    12961 attttttgaa gcaaaatttg catttatttc gagtaaataa aatagaataa aaacaaaaaa
    13021 acccaaaatt actttcgggc ctttgctcct aaaaatcagg gaaatatcat taatctttaa
    13081 ctcattattc tgttggatta tcatttgttt gtgaagattc atcttcagga ggattttctt
    13141 tttgagcttt tgcagcttcg gctgcttttt tctttgctaa ttcttccgac tttttcttgt
    13201 tttgttctgc ttcggcagct aaacgtgctt tttcttcagc ctgttttttc ctttctactt
    13261 cgttaatgtt catattaatt tttgcttctt tgaaagcttt ccatgcttca aatcttcttt
    13321 cggcttctgc ttcatcaaaa gctccttttt ttacgcctcc aagcaaatgt ttttttaaca
    13381 atactccttc ttgcgaaagt agattgcgtg ctgtatctgt tggctgagct ccaacttgca
    13441 accaatacaa ggcacgatca aattttaaag ctactatagc cggattcgta ttaggattgt
    13501 aagaaccaat ccgttcgata aatttcccat cacgtgggga acgactgtct gctacgacaa
    13561 tatgataata tgcgtaacct ttgcgtccat gtcgttgtaa tctgattttt actggcattt
    13621 ttatacattt tttaaaatta tacctattgc tttttctcga aaagcggtgg caaagttaat
    13681 gtatttcttc gagatacgga aatttttttc ttttaaaaat tatttgtcga taaaaaccca
    13741 cgcagaagca aattgcggtt cggaatgatg aaggtgatgc aaaaattcta ctgcatcatt
    13801 atattcgata aatgattgaa gagcaacctt ataatttttt tgaggggata taacatgtgc
    13861 tttttcaaag cctatactgt gaagagattc gcaaaatttt tcagcagctt tttctgtata
    13921 aaaacttgct actatgacgt gataaggaca tttaagtgtg gtagcagtat tttcttcttg
    13981 ttcagaagtt aacagctcag aaatagtatt tatcacttgc gaagaagaat ttacagaagt
    14041 tggtttttct atgattttct ccgtttgtga tgaacgtttc atttctaatg ggaaagaagc
    14101 agtataaaaa cttctccgtg tatcggctat acgaggagat accaacaata aaacacaaac
    14161 cagcgcagta gctgccacat ttctcattga agaagctgat ggcaatgtta tagtaacttt
    14221 cttattttga ctggaagaaa tagatttttt aggaaaaaat tgaatatcgg tcattccaaa
    14281 attagcaggt aaaaaatcaa ctcgttctgc tggaacaaaa actaacttac ctatctcatt
    14341 tcgtttcaaa aaacccagct tgcctaatga aacaattttt gctgattgaa gctgaaaatg
    14401 aatcaattct acttcctttc ttatccattc taaagccgac tgataactga tcccttcttc
    14461 ttgagcaatt gctatggcta ataacccatc gctatctttc aacaaaggat taaatcctag
    14521 tgttaacaat ggaggaacaa ttctatcttg caaaattcga gctgattgat tttgaaccat
    14581 aaaaccgccc aaatctggta caattacgta atcataacgt accaataatt tttcaacata
    14641 ataactcaat ttttccatat tgcaaatata acaagtaaga aaataaaatc ccaatatttt
    14701 ttgtgaaatt tttccacatt ttgtccattg caaaaaaaca atttatcttt gtaataagta
    14761 aaagtaaaaa cttcacaaat gaaaagaaca atattaatta caggtggagc aggttttata
    14821 ggttcgcacg ttgtcaggct gtttgtcaat aaatatcctg aatatcaaat tgttaattta
    14881 gatgctttaa cttatgccgg caaccttgaa aatttaaaag atgtatcgaa cgcacccaat
    14941 tatatattta tcaaaggaga tattcgggat aggaaactga tacctgaatt atttactact
    15001 tatcagtttg atggagttat tcatttagct gccgaatcac acgtagatcg gtctataagt
    15061 gatccttttg catttttgga aacaaacatt tttggcactg tcaatcttct tgatgcagcc
    15121 aaaaaagcct ggaaaaacga tatggaagga aaacttttct atcatatttc caccgacgaa
    15181 gtatatggtt cgttagatga aaacggatat ttttatgaag gaactgctta tgaccctcat
    15241 agtccgtatt cagcttcaaa agcaagttct gatcattttg tttctgctta ttatcataca
    15301 tatggtttac ccgttatcat ttctcactgt tccaacaatt atggagcaaa tcaatttcct
    15361 gaaaagttaa ttccactttg catcaataat attaaaaaca aaaaatccat accagtttac
    15421 ggaaaaggtg aaaatgtgcg tgattggctc tgggtgaacg atcatgccgc tgctattgat
    15481 cttatttttc ataaaggaaa agtaggtgaa atttacaaca ttagtggaaa caatgaatgg
    15541 aaaaatattg atttaatcaa aaaattatgt gaaataatgg atcggcaatt aggtagagca
    15601 gaaggagaat cagaaaaatt aattactttc attaaagacc gtccgggaca cgatttacgt
    15661 tatgcaattg attcttccaa actgcaaaaa gaattgggct ggaaaccttc cgtaaatttt
    15721 gaagaaggat tggaaaagac agtagagtgg tatctggaga atgaagaatg gctcaataat
    15781 gtgataaatg gtgattattt gaaatattat gaaaaacaat atcaaaacca agaaaacgaa
    15841 aaatgaattt atttcttttg aaaataattc tttcgcattt cgtttacttt tatagtaagt
    15901 cgtaatcaaa aatatcgttt aactttgaaa tagtatgatg ttgatttcgc aaatagatga
    15961 tgaaatgcta ttttttaaga tggagtttcc ccctccaaca aatgtttgat tgttaaacta
    16021 aattgttatg ctgttttttt tttaaaaaaa tactgaaagt aagaaattct tatctaattt
    16081 ttataaacaa aatactataa actcacacaa aaaatggtct aaattttcct ccatgtaact
    16141 ccgcaccttt tctatgaaac tctatgaaaa tgaatgattt attagatgga aaaacacaga
    16201 gattcacaca gaggaatacc gagatttttt tcttattttt aaatgattaa atccttttgt
    16261 tttttaacat tttacgcatg aataaaagac gctttgcgaa gaatctctac tggaaactca
    16321 caaattcgaa actcgaaacc tgtcaatttt cagctcaccg ggcgaagtca atttataaac
    16381 aagagggtta atcaataaat atcaatttga tataaaaaat attttttatc atatgctaaa
    16441 aaacaaaata tttgtaagaa ttttcaatat tttacagaat atagctttta ctttatgtca
    16501 taatgtttat ttctaatgat aattctgagt ttaaataaat tattaattcc acattttggt
    16561 aattcaaatt tacaaacaag aaaggcattc tgaaagatat tgtggtgaaa aatatttata
    16621 aaaataggta ttaaatatat tttctgaata aggcattttc aagcttttac tgagtcgaat
    16681 gcaaataaaa acttgttatt ttcaaaatta aattttaagt ttgaaatcta ttatttagat
    16741 gaagtttttt aaaaaaatgt aagagttgta tttagttatt taattgattt ttatctttaa
    16801 tttaatcaat atggatttat ttaatgttta tccccttttt aatattgaaa ttgtaaaagg
    16861 tttaggttgt agaacttacg ataagcaagg gaatgaatat ctggattttt atggtggtca
    16921 tgctgtaatt tcagtcggtc attctcatcc ttattttgta aagaaattaa ctgaacaaat
    16981 acaaaaaatt attttttatt ccaattcagt aataaatcct ttgcaaatta agcttgctga
    17041 caaattagga aaaatttctg gttatgagga ttattccttg tttttaatta attcaggagc
    17101 agaagccaat gaaaacgcct taaaattagc ttcttttcat acaggaagaa aaaaagttat
    17161 tgcttttagc aaaagttttc atgggcgcac ttctgctgct gtcagcgtaa cagataatcc
    17221 ttctattgta gctcccatta atgagaattt tgaagttgaa tttttccctt taaatgatac
    17281 agtggcagta aaaaaatctt tgagaaaaag ggatgtttgt gcggttatta ttgagggaat
    17341 tcagggagta ggaggtatac aaattccaaa tgcagatttt ttacaagttc tcagtgcaga
    17401 atgtaaagct acaggtacaa tacttatttt agacgaagtc caatcaggtt atggaaggac
    17461 gggtaaattt tttgctcatc aatatgctgg catccgccct gatcttatca ccgtcgcaaa
    17521 aggaatgggg aatggcttcc ctattggtgg agtaattatc agccccatgt ttaaacctat
    17581 ttatggtatg cttggaacta cctttggagg aaatcatttg gcttgcactg ctgctcttgc
    17641 agtgctggat atcatagaag aagaacactt gatggaaaat gctaaaaaaa taggtgatta
    17701 cctcttggat gaattacaaa aatttcctcg aattaaagaa gtaagaggag taggattaat
    17761 gattggtatt gaattcgaag aacctgtaag tgctttaaga acaaaattat tgtatgacaa
    17821 aaaaattttt acaggagtga gtggttttaa tattattcgt ttacttcctc cattgtgtct
    17881 ttctaaagaa gaagcagatc tgtttttgga acgtttttat gaattacttt aatttgtcgg
    17941 atgaaaactc attttttgtt attaatagat ttttttattt ttctttctct cttaaattta
    18001 tctgcacaag agacagttca tgataaactt attttaaaaa gcggcggtat ttatgtgggt
    18061 gaaattattt taaaaaacaa cgaagtagta atattacaaa ccaccgatgg aactcgatat
    18121 caatttcctt tttctgatat taaagaaatt agtaaatcat ccgatatttt cccaacaaaa
    18181 aatatatctt ctaacaatga taccctctct tttccttttt gtgctttttt agaatttacc
    18241 ggtggagtag cacaggcaaa aaataaattt gatttatcac ctaattcaca aataacatta
    18301 atttttggga aaaagcaatt gttcaataag ggctttttca ttggtggtgc gataggatac
    18361 aacaacactt cgctggaaca aaataattct ttgaactttc taccaatttt tattcgttta
    18421 gaagacattc ccataaaaaa gaggttgtct ccttatatga cattagatgc tggttatgct
    18481 tttgctcttg cttctgatta cagcggtgga acaatgacta aaatttcagg aggaattacc
    18541 tataaactta cgtataa
//
LOCUS       DAOSAS010000021        18274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_2128_length_18274_cov_13.733246, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000021 DAOSAS010000000
VERSION     DAOSAS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18274
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2128_length_18274_cov_13.733246"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="PLR72_01850"
     CDS             <1..1097
                     /locus_tag="PLR72_01850"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOK35988.1"
                     /translation="KKYRKYKKTLLARKNIIDACAMYRRIDALKIGGYCEEIIGREDW
                     EFWISLLKNGGEVYHLPEVGVYYRIRQHSKRVTDRRLKKHIIDVLNRRHKAFFYRELG
                     GKLHYQRSLSRLLNFFIHLFRPEAFVISPDFKKEEEFFYSLPDIFPHQGETIHSGRNI
                     IKIFSIGTQKWVVKWFKSPNFFNRFIYGNFRKSKARRSYEYALKLQSLGIKTPVPIGY
                     YEQKNLFFFSQSFYATLASECEHRFDEIIANKDFKDRELILKEFATFSAKLHENNVLH
                     GDYSEGNILFSIKGKEVLFEIVDLNRIKFKKVSPEEGCKNFERLNLEPEALTIIAKAY
                     ARARNLDEKNCIDWILKYRWRKHKNISKTI"
     gene            1116..2507
                     /locus_tag="PLR72_01855"
     CDS             1116..2507
                     /locus_tag="PLR72_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35989.1"
                     /translation="MIRKLSAGDRFVISNFFVAFVFFWIIRTIYHFTTITNEQNIPFL
                     TIIAILFFIINLTLSYFIFKKERAKIKFSWEFILNMALLFLIISTVLSVYTSIVATVA
                     FAGLVIIYASKKKIHFQPHPLFYFLVAYYLLQYIGLIWSIDPQNGFRFVGKGLSFIIV
                     PLAFSFYSISEDEKRKLLRIFFRLLEIYLLMILLAYCYQVYFHKVNFGIGFHLKKNYF
                     PTPIPPGETHHLLLAWAGYNHPTFVSFILTLMFGIGFYLWRNDNSPQTVRISGFEVGF
                     YGFMTAGVILLLQSRIGMLMFPFGIFLTLLSSLRKNKKLVLSVIVFSALLVLLAGFYI
                     FKNYRGYFYDHPREIQTDIVLSFLKDHYLLGTGTGGMKVLIPWYPTAHNQFLGDLFHL
                     GIPGLLLLLALMGSSFYFAIKDKNFPLLYFLLIYFVMMQIEMPLSLQKGITYYTLFVC
                     LFLNGKKKATTKE"
     gene            complement(2515..3147)
                     /locus_tag="PLR72_01860"
     CDS             complement(2515..3147)
                     /locus_tag="PLR72_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HOK35990.1"
                     /translation="MSKNKFYVVWKGKQPGIYSSWEECKLQIDGIKDAIYKSFLTEEE
                     AIEAFKSPSSNYIVKNLSFSEKKSDEKNSLLQPILESISVDAACSGNPGILEYRGVYT
                     RTGKEIFHQGPFQNGTNNIGEFLAIVHALALQMKKNTSFPIYSDSATAIKWVKTKKAK
                     TKLEESPENAYLFELVERAEKWLEQFEYSIPVLKWQTENWGEIPADFGRK"
     gene            3238..3309
                     /locus_tag="PLR72_01865"
     tRNA            3238..3309
                     /locus_tag="PLR72_01865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3271..3273,aa:Arg,seq:cct)
     gene            complement(3272..4027)
                     /locus_tag="PLR72_01870"
     CDS             complement(3272..4027)
                     /locus_tag="PLR72_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK35991.1"
                     /translation="MNSSSNIDFQSLQNNKIIIAKNIHKSFGNLEVLKGVDLSVNEGE
                     VIAIVGPSGAGKTTLLQILGTIDKPNEGKVIVNGIDVAKLNERELSVFRNQQIGFIFQ
                     FHQLLPEFTALENVMIPALIAKKDAKETVKRAKEILSELQLADRMEHKPNELSGGERQ
                     RVAVARALMNQPSVILADEPSGSLDSKNKIELHQLLFDLRAKFGLTIVIVTHDKELAK
                     LTDRVIELKDGMIQNVNNNGKVVLPGIEPESKV"
     assembly_gap    4034..4133
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4134..5233)
                     /locus_tag="PLR72_01875"
     CDS             complement(<4134..5233)
                     /locus_tag="PLR72_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOK35992.1"
                     /translation="MTRKEKVFFDKLGIVTAATGSAVGLGNIWRFPYLLGQSGGGAFL
                     VVYLICVIFLGLPVMISEFSIGRMAQANAVTSFRSLAPNKKWWLIGVMGVLSAFFILG
                     FYMVVSGWTLEYIVQAITNSFANQNVADLSQAFTDFSTNTWRPLIWIVLFTAINCAIV
                     IAGVKKGIEKTTKFLMPLLFIIIITLGIRSVTLPRGINGLNFLFKFEFDKITSKVILS
                     AMGQAFFSLSLGMGCMITYGSYMNKSNNLSRTALEITLLDTLVAILASVAIFPAVFSF
                     GIDPTQGPQLVFITLPNVFPKMPGGYIWAILFFVLLALAALTSTISLLEVIVAYLNEE
                     FNVSRKTAAIGSSVLIFILAILSSFSMGIWKNV"
     gene            5405..5992
                     /locus_tag="PLR72_01880"
     CDS             5405..5992
                     /locus_tag="PLR72_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOK35993.1"
                     /translation="MVKPFVFVTNNPHKVEEVRAILSPSIKIVSLSDLGFFEEIPETA
                     DTLEGNALLKAKYIYEKYGLNCFADDTGLEIEALGGEPGVFSARYAGEGHNSYENRKK
                     VLNLMTHQTNRAACFRTVIVLILEGKKYFFEGKVKGIISEEPRGNGGFGYDPIFIPEG
                     FNKTFAELTAEEKNQISHRALALKRLSDFLEKINH"
     gene            6145..8427
                     /locus_tag="PLR72_01885"
     CDS             6145..8427
                     /locus_tag="PLR72_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOK35994.1"
                     /translation="MLKAENLAMGKWRTHLAYNSISRIAQSDDKIYATGDNALFSVDK
                     RDGSIELYSKISGLHDNVVGNFEYDNISKQLLIVYANGNMDIMGSGGIINIPDLYNKP
                     QISDKQINHIYFYGNKAFLSTNFGIIVVNIQKNEIADTYYIGPNASEVKVLATTVSNG
                     YIYALTEDAIYQASVNEPNLVNYQFWTIDNTIPGSGNFQAIVSFAGQLFILRNNKIYK
                     KDSNNLWTTFYSEITATNLSVSNGKMIIYTTTNVYVVDEAMNFKTISGLGKILDVEYD
                     TNNNLYWLAANSQGVVSYQENSGGTPIISTYKPSGPAVNIPWKMRFSGQKLFVVPGGR
                     WSAQNNQPGYVMIYENGLWTNIDGKDIEAQTGKSVLDFMDIAIDPADPSHFYIPSYGT
                     GVYEFKNNQFAAWFNFNNSTIETIFPDNPNSAYLYMRMDGGVFDTEGNVWFTNSLVLN
                     GIKFRKPDGTWSKLTYPSLLSKETLGELMILKQKTQQKWVLSVRSAPGICIFDDNGTL
                     DDQNDDKSIFYSSFQTPDEKITPTSYYCLTQDKNGVVWVGTDMGPLLFYNPTNAFNSS
                     EFMASRVKIPRNDGTNLADYLLADEKVKAIAIDGANRKWLGTESSGVYLMSENGQETI
                     HHFTTLNSPLLSNTILSIAINPVTGEVFFGTGNGIISYQSDAVEAGDTFGDVYAYPNP
                     VRENYNGIITITGLVDKTHVKITDLNGNLICETISNGSIATWDGKDAHGRRVSTGIYL
                     IICTNSEGSQSAITKILVIN"
     gene            8845..10005
                     /locus_tag="PLR72_01890"
     CDS             8845..10005
                     /locus_tag="PLR72_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35995.1"
                     /translation="MNNLITKNYKYNGKTLLLCIIYLIINGLFIFKYIRRVNFVDSLI
                     VFFIYILFILTFFIINQKLQKKDKLLKFFLISLLIISLALIIFIQIKINPLTLKVDRW
                     SAINNFLSNLFKGKSPYSANTHLGGYGSPFPVWQIFHIPFYLLGDVGLCMIFCFVCLI
                     LCVKWYSNSNKVTFTFLSLMALSPAFWYEVSVRSDLMYNFFVVFIALLIIQKKNFNLQ
                     NNTISLGIVCGLFMSTRLSVFLPLFLYFFYEFWTINLKKKILFIVSGLVTFILSFFPF
                     VLWDYKTLFLTNHNPFILQTRQGFGIDIIIIIALAIYFSFKAKNNFYKIIIFISFSLF
                     ILVAINFLFRLANENFADLLFDSGYDITYFNMSLPFIIAGISLINRAMDADL"
     gene            10140..11324
                     /locus_tag="PLR72_01895"
     CDS             10140..11324
                     /locus_tag="PLR72_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35996.1"
                     /translation="MQNKVTHTLSNCPLFYLKKQWWIFLIFISLFIKIFLLPVKTGDY
                     VVYLEPWINFIKTHGYFSALKYDFYNYTPAYLYILAAIAKIGLNPLFLIKGISVLFEY
                     VTAYFVGKIAWLKFKDNLVIIIALAIVPLLPSVMLNSSYLSQCDAIYASFTVASIYYA
                     LKNKSFLSILMLGIAFSFKLQAVFILPFFFVMLLRGNVKWYHFFLIPTVYLISIFPAW
                     LVGRPFSELISIYFSQADYYRLLTMNFPNLYIWFNNNYYELISKAGIFFTFLFTLFGG
                     IKLSLKRINFTFENWIQLAFLSVTVIPFFLPGMHERYLYLGDVLGVAYLMIFPRKFYL
                     PLAVWVISLYSYIRCSRFNEILPMWPAFFIYLLILVFISIDFYLGIRTNSSLSKTNEI
                     IL"
     gene            11380..12822
                     /locus_tag="PLR72_01900"
     CDS             11380..12822
                     /locus_tag="PLR72_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK35997.1"
                     /translation="MSLKIRKQFLFIFFIISIYTLLTIVCWNHCYFWDNIQQISKEAH
                     WFYQNNFSSLIIPIHSSSDEIVPTGYHPPLMGIMTAALWKIFGYHLWVSHFFVFCWFF
                     ILLYQTIKLLQLIFPQKYASGALIIVLLEATVLTQFAIASPDFILLTAFVICLRAILE
                     QKKILLSVGLIFLCGINARGLLTGIALFFTNYYYLYLQSEKKHTFECLKKSFIPFLPT
                     LLLLIAYYCLYFKTNGWFFADSSEAGHYALPANFSMIIKHLFEFIIRLIENGRIVVWG
                     IAFYLFILIIRGKIQTSIKDNVLIFCFLFLMAEYLVLVFTTRMPFSGRYFMPLFFVLT
                     LITLSGITHFFDRKKIKITFVVILLFELTGHFWIYPEKIAQSWDSTLAHLPYYELRKN
                     CFDYIDQQGIDYQKISAGFCLYGDRGYIELQHAGKKVNDEFNNEYFIYSNISNIADEW
                     LFQLKDTTQWKPIQTFQKWPVKIILYQKIQ"
     gene            12973..13359
                     /locus_tag="PLR72_01905"
     CDS             12973..13359
                     /locus_tag="PLR72_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOK35998.1"
                     /translation="MNYSKIVIQFFKFGIVGILNTLITAFIIFLLLKVFHCSDYVSNI
                     IGYLAGLINSFIWNRKWTFESHGKISVTVFKFIITFVISYLLQLGGLHLLLQYTTIDN
                     YVCQLIAMVIYTIINFLLNKYYTFKI"
     gene            13365..14324
                     /locus_tag="PLR72_01910"
     CDS             13365..14324
                     /locus_tag="PLR72_01910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK35999.1"
                     /translation="MYQSLSIIVPCYNEEEVIIESYKRLKQVSQKLDFDTDIIFINDG
                     SIDRTEQILSEIAKTDKNVKVISFSRNFGHQNAVTAGLNHCTSDLAVIIDADLQDPPE
                     LIPQMIETLKKNQAQVVYCVRKIRKGESLFKRFSAKIFYRFLNFLSEIKLPVDTGDFR
                     LVDKNIIKEFNRMREKGKYIRGLIAWMGFKQVPFYYEREARFAGETKYSLSKMIKLAT
                     TSMLYFSKKPLKIAIYLGFFTTLLAILLVLWSIFGKIFGFTNADPGWTSLFIAIVFFG
                     GIQLLTIGILGTYIGTLFDEVKDRPEYVVKNLLNFENNIEKEK"
     gene            complement(14448..15365)
                     /gene="metA"
                     /locus_tag="PLR72_01915"
     CDS             complement(14448..15365)
                     /gene="metA"
                     /locus_tag="PLR72_01915"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOK36000.1"
                     /translation="MPLNIPASMPAVEILRKENIFVMDSDRASHQDIRPLKIVILNLM
                     PLKITTETDLIRLLSNSPLQIEVDFLQLESHTPKNTPIEHMMAFYKKFDVIKESNYDG
                     MIITGAPIEHLSYEEVTYWDEITKIFDWVRTHVTSTMFVCWAAQAGLYYYYGIPKYPL
                     KEKMFGVFEHTLYNPQNPIFRGFDDVFYVPHSRYTEVRAEDIKKVPELTLLSESAESG
                     VYMVMGRNGREFFITGHSEYSARTLDDEYKRDLAKGLPIKMPKNYYLNDNPENEPVVR
                     WRSHANLLFTNWLNYFVYQETPYDLSKIH"
     gene            complement(15456..16250)
                     /locus_tag="PLR72_01920"
     CDS             complement(15456..16250)
                     /locus_tag="PLR72_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOK36001.1"
                     /translation="MKIKKYIFFTIAFVLLILFIKLLFFREKTAHNLPAILKSGRLSV
                     VTDSSALGFAMKGDSVFGFQYEIAKAFADSLNLELIISTNNNFKECLEGLRKGNYDIY
                     ADFIPITSEWQNEYLFTIPLKTSRQVLIQCKDSSEKIIKNQVDLAGDTIYIPKNSPHK
                     LRLKNLSDEIADTIYLVEVNDVNSEDLVKWVAMGKIKQTICDERDALKWKLQYPNLDI
                     SLPVGFDQQYGWMLYKESKQLQKKLNDFLNDFIGSEAYWKIYRKYY"
     gene            complement(16314..18194)
                     /locus_tag="PLR72_01925"
     CDS             complement(16314..18194)
                     /locus_tag="PLR72_01925"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOK36002.1"
                     /translation="MPIKKHLVFCILFFFTNSIWGTIPVKPRPVKTWKISDPFAVVDT
                     IPVDTLHLNFQDANPIDRFSIANSYNGNLGSPIEPKLYFDRPEKSDFIFSEAFYPYIM
                     DIEKATFYNVKMPYTHIVYNTGGSTYRQQDHIKFLFTANANKSLNFGTNLDYFYAVGE
                     YNNQAAKRFSGSLFGSYNGKHYSATGLVTVNNMFNHENGGIADSSYIYEPLGIATKDI
                     PTRINGYSVYRKNVFFYNQQYTIGFERPIHISPDSVKMEYVPVTRFAHTIKYEDPRKR
                     YYEPTVEKDFYQNTYLPFTQTNDTAALQSLTNTVSVNMAEEFNKWLKFGMTAFLQNEV
                     QRYTFKIDSVLERKNMSSTKVGGILSKYKGPRFRYEILGEMGLLGYKTGDFQIKGKLG
                     GYFKLFKDSIILTAGGFVKSDEPSYFLQHYHSNHFCWDNNFNKTYRTNINGTFSIPTR
                     YFSLNVSVENLTDYIYFNSQALPVQHNGNVQVLALNLKKDFHFGKFNLENNIVYQRSS
                     NSEVIPLPAFALYHNLYYNGLWFNVLSAQLGANLRYFTEYYSPCYMPATAQFYNQSTT
                     KVGNFPVINVYANFHLKQARFFVEYYHINQLFMKTAYFSMPNYPLSPAIFKIGVSTNF
                     YN"
BASE COUNT         6035 a         2832 c         2811 g         6496 t
ORIGIN      
        1 taaaaaaata tagaaaatat aaaaaaacat tgctcgctcg aaaaaacatt attgatgcct
       61 gtgccatgta tagaagaatc gatgctttaa aaattggtgg ttattgtgaa gaaattattg
      121 gacgagaaga ttgggaattt tggatttcgt tgctgaaaaa tggtggggaa gtttatcatt
      181 taccggaagt tggtgtttat tatcgtatac gtcagcattc aaaacgagtg accgatcgtc
      241 gtttaaaaaa acatattatt gacgttttaa accgtcgtca caaagcattt ttttatcgcg
      301 aattgggagg aaaattgcat tatcaacgct cgttatcacg cttattaaat tttttcattc
      361 atctttttag acctgaagct tttgttattt cacctgattt taaaaaagaa gaagaatttt
      421 tttattcttt gccagacatt tttcctcatc aaggcgaaac aattcattca ggaaggaata
      481 tcattaaaat tttttcaatt gggacccaga aatgggtcgt gaaatggttt aagtcaccaa
      541 atttctttaa tcgctttatt tatggaaatt ttagaaaatc gaaagcacga agatcttatg
      601 aatatgcttt gaaacttcaa tcgttaggga taaaaacacc cgttcctatt ggttattatg
      661 aacaaaaaaa tttgttcttt ttttctcaaa gtttttatgc tactttggca tctgaatgtg
      721 agcatcgatt tgatgaaatc attgcaaata aagattttaa agatagggag cttattttaa
      781 aggaatttgc aacgttttct gctaaattgc acgagaataa tgttttgcat ggtgattatt
      841 ctgaaggaaa tattttattt tctatcaaag ggaaggaagt tttatttgaa atagttgacc
      901 tgaatcggat aaaatttaaa aaagtatcac ccgaagaagg ttgcaaaaat tttgaaagat
      961 taaatttaga acctgaagct ttaactatta tagcaaaagc atatgccaga gcacgaaatt
     1021 tggacgaaaa aaattgtata gactggattt taaaatatcg ctggagaaaa cataaaaaca
     1081 tttcaaaaac tatctgatca tttaaaagtt gaattatgat tcgtaaactt tctgcaggag
     1141 atcgttttgt tattagtaat ttttttgttg cttttgtttt tttctggatt attcggacaa
     1201 tctatcattt taccaccatt accaatgagc aaaacattcc ttttctcaca ataatagcca
     1261 ttttgttttt tattattaat ctaaccttat cttattttat atttaaaaaa gaaagagcta
     1321 aaataaaatt ttcatgggag tttatattaa acatggcttt gttgttttta attatctcaa
     1381 cagttttaag tgtctatacc agcattgtag caacagttgc ttttgcagga ttggtaataa
     1441 tttatgcttc aaaaaagaaa attcattttc agcctcatcc attgttttat ttcttagtag
     1501 cttattattt acttcaatat attgggttaa tttggtctat tgatccgcag aatggtttcc
     1561 ggtttgtagg gaagggatta tcttttataa ttgtaccatt ggcatttagt ttttattcca
     1621 tttcagagga cgaaaaaagg aaattgttaa gaattttttt ccgcttgttg gaaatttatt
     1681 tattaatgat attacttgca tattgttatc aagtatattt tcataaagta aattttggaa
     1741 ttgggtttca tctgaaaaaa aattatttcc caactccgat tcctcctgga gaaactcatc
     1801 atttattgtt ggcgtgggct ggttataacc atcctacttt cgtttcattc attttgacgc
     1861 tgatgtttgg aatcgggttt tatttgtggc gaaacgacaa ttcaccgcaa acagttagaa
     1921 tatccggttt cgaagtaggt ttttatgggt tcatgacagc cggagttatt ttattgttgc
     1981 agagtcgtat aggcatgctt atgtttccgt ttggaatttt tttaaccttg ctttccagct
     2041 tgagaaaaaa taaaaagctt gttctttcag taattgtttt ctcagctctt ttagttctgt
     2101 tagctggatt ttatattttt aaaaattatc gtgggtattt ttacgaccat cctcgcgaaa
     2161 ttcaaactga cattgtactt tcgtttttaa aagatcatta tttattaggc actggtacag
     2221 ggggaatgaa agttttaatt ccctggtatc caaccgctca caatcaattt ttaggagatc
     2281 tgttccattt aggtatcccc ggcttgctct tgttactggc tttaatggga agttcgtttt
     2341 attttgccat taaggataaa aattttccgt tgctgtattt tctgttgatc tattttgtta
     2401 tgatgcaaat agaaatgccg ctttctttac aaaaaggaat tacttattac actttatttg
     2461 tctgtttatt tcttaatgga aagaaaaagg caacaactaa agaataaaaa ttttctattt
     2521 tcttccaaaa tcagccggaa tttcacccca gttttcggtt tgccatttta aaacaggaat
     2581 agaatattca aattgttcca accatttctc agctctttca actagctcaa agagatatgc
     2641 attttcaggt gattcctcca attttgtttt tgcttttttt gttttcaccc attttatggc
     2701 agttgcacta tcagaataaa ttgggaaaga agtatttttt ttcatttgta aagccagtgc
     2761 gtgcacaatt gctaaaaatt caccgatatt atttgttcca ttttgaaacg gtccttgatg
     2821 aaagatttcc ttgcctgttc tggtataaac tcctcgatat tcgagtatac caggatttcc
     2881 actacaagct gcatctacgg aaatactttc caagattggt tgaagtaagc tatttttttc
     2941 atctgatttt ttttctgaaa aagaaagatt tttcactata taatttgagg aaggagattt
     3001 aaaggcttca attgcttctt cttctgtaag aaaagattta taaatagcat cttttatacc
     3061 atcgatttgc aatttacatt cttcccacga agaatatata ccaggttgtt ttcctttcca
     3121 gacaacataa aatttatttt tgctcataaa atttaacctt atacaatggt ttcaaaatag
     3181 gatagaatgc ttttctctct cattaaaaaa acgtaatttt gcactacttt cccaaaaggt
     3241 cctgtagttc aatggataga acaaaagttt cctaaacttt agattcgggt tcgattcccg
     3301 gcaggactac tttcccattg ttatttacat tttggatcat cccatctttg agttcaatta
     3361 ctctgtcagt taattttgct aattctttat cgtgtgttac aataacaatt gtcagtccaa
     3421 actttgctcg taaatcaaac aacaattgat gcaattcaat tttgtttttt gaatccaaac
     3481 tgcctgatgg ttcatcagcc aaaattactg aaggctggtt catcagagct cgagccactg
     3541 ccacacgttg tctttctcct cctgacaatt catttggttt atgttccatt cggtctgcca
     3601 attgtaactc acttagaatt tcttttgctc tttttacagt ttctttagca tccttttttg
     3661 caatcaaagc tggaatcatc acattttcaa gagcagtaaa ttcaggtaac aattgatgaa
     3721 attgaaaaat gaatccaatt tgttgattac gaaaaacaga taattctctt tcatttaatt
     3781 tggcaacatc aatgccattt acaataactt taccttcatt cggcttgtca atcgtaccaa
     3841 gaatttgcaa taaagtagtt ttaccagcac cgcttgggcc gacaattgca attacttctc
     3901 cttcatttac tgataaatca acacctttta atacttcaag attaccaaaa cttttatgga
     3961 tattttttgc aataattatt ttattgtttt gcaaagattg aaaatcaata tttgaagaag
     4021 aattcataaa attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacatttt
     4141 tccatattcc cattgaaaaa gaagacagaa tagccaatat aaatatcaac acggatgagc
     4201 caatagcagc agttttccgg ctcacattga actcttcatt cagataagcc acaattactt
     4261 ctaatagtga aatggtagaa gtcaaagcag ctaaagccaa taaaacaaaa aataaaattg
     4321 cccaaatata tccgcccggc atttttggaa aaacattagg aagtgtgata aatactaatt
     4381 gtggtccttg cgtaggatca atgccaaagg aaaaaacagc aggaaatatt gcaacggaag
     4441 ccagaatagc aaccaaagtg tccagtaacg ttatttcaag tgcagttcga cttaaattat
     4501 tacttttatt catataagaa ccatacgtaa tcatgcaacc cattcccaaa cttaatgaaa
     4561 aaaaagcctg tcccattgca cttaaaatta ctttggaagt aattttatca aattcaaatt
     4621 tgaacaaaaa attcaagcca tttatacctc tgggcaacgt aacagaacgt attcctaaag
     4681 tgataataat aataaacaaa agaggcatta agaacttggt agttttttca attccctttt
     4741 ttactcctgc gatcacaata gcacaattaa tcgctgtaaa taatacaatc caaatcagcg
     4801 gtcgccacgt attcgtgcta aaatcagtga aagcttgact caaatctgct acattttgat
     4861 tggcaaaaga attggtaatg gcttgaacta tatattccag tgtccatcca gaaaccacca
     4921 tataaaaacc aagtataaaa aaagccgata atactcccat tacaccaata agccaccatt
     4981 ttttattggg tgccagacta cgaaaagaag taactgcatt ggcttgagcc attctgccaa
     5041 tggaaaattc tgaaatcatc actggtaatc cgagaaatat aacacatatc aaataaacca
     5101 ctaaaaaagc accacctccc gattgcccta acaaataagg gaatcgccag atatttccta
     5161 atcctacagc agagcctgta gcagcagtaa caattcctaa tttatcaaaa aagacttttt
     5221 ctttccttgt cataaaaaat gaaaaatgta ctctttaatt tggttacctt aaaaaattgt
     5281 cacaaaaata atgaaattta ttgagtataa gaagaaaggg gaaatccaaa atataatata
     5341 aaaatactga cattttaaat tatacaaatt tattatcttt gcagaaagta gttaaagaaa
     5401 gaaaatagtg aaaccatttg tttttgtaac gaataatcca cataaagtgg aagaagtaag
     5461 agccatttta tcgccttcta tcaaaattgt aagtttgagt gatcttggtt tctttgaaga
     5521 aattccagaa acggctgaca ctttagaagg aaatgcgtta ctcaaagcaa aatacatata
     5581 cgaaaaatat gggctcaatt gttttgctga tgacacaggt ttggaaattg aagctcttgg
     5641 aggcgaaccc ggtgtttttt ctgcccgtta tgctggtgaa ggacataatt cctatgaaaa
     5701 cagaaaaaag gtattgaact taatgactca tcaaaccaat cgagccgcct gttttagaac
     5761 agtgattgtt ttaatattgg aagggaaaaa atattttttt gaaggaaaag taaaaggaat
     5821 tatcagtgaa gaacctcgtg gcaacggtgg ttttggttat gatccgatat ttattcctga
     5881 aggttttaac aaaacttttg cagaactgac tgccgaagag aaaaaccaga ttagtcatcg
     5941 agcattggct ttgaaaagac ttagtgattt tcttgaaaaa atcaatcact aaatattttt
     6001 gtagagctat cgcaaaataa atcaaacaaa aaattgttta tacataataa aactcattga
     6061 aaattattca cttaattagc tcatatcatt aaaatgaaaa aaaatacaat tttccttttc
     6121 ttactattgt cagttttttg taaaatgcta aaggctgaga atttagctat gggtaaatgg
     6181 agaacccatc ttgcttataa tagtatttcc agaattgctc aatctgatga taaaatttat
     6241 gcaacaggag ataatgcttt attttcagta gataaacgag atggaagcat agaactttat
     6301 agtaaaattt ctgggcttca tgataatgta gttggaaatt ttgaatatga taatatttct
     6361 aaacaattac tcattgtata tgccaatggc aatatggata ttatgggatc aggtggcatt
     6421 atcaatattc ctgatttata taataaaccc caaatttcgg ataaacaaat caatcatatt
     6481 tatttttatg gtaataaagc atttttatct acaaattttg gcatcatcgt agtcaatatt
     6541 cagaaaaatg agattgctga tacttattac attggtccaa atgcttcaga agtaaaagtt
     6601 ttggctacaa cagtttctaa tggttatatt tatgctttaa cagaagatgc tatatatcag
     6661 gcttctgtca atgaaccaaa tttagtgaat taccaattct ggaccattga caatactata
     6721 ccgggaagtg gcaattttca agctattgtt tcgtttgccg gtcaactttt tattctccga
     6781 aataacaaaa tttacaaaaa agatagcaac aatttatgga caacttttta ttccgaaatt
     6841 acagctacca atttgagcgt ttctaatggg aaaatgataa tttatactac cactaatgtt
     6901 tatgtagttg atgaggcaat gaattttaaa acaataagcg gattaggaaa aattttagat
     6961 gtggaatatg ataccaacaa caatttatat tggcttgctg caaacagtca aggagtcgtt
     7021 tcctatcaag aaaatagtgg tggaacgcct attatttcta cttataaacc ttcaggacca
     7081 gcggtaaata ttccctggaa aatgcgtttt tcgggtcaga aactttttgt agttcctggt
     7141 gggcgatggt ctgctcaaaa taaccagcca ggttacgtta tgatttatga aaatggttta
     7201 tggacaaaca ttgatggaaa agatatcgag gctcaaactg gtaaaagcgt gcttgatttt
     7261 atggatattg ccatagaccc agctgatcct tctcattttt atattccgtc ttatggaaca
     7321 ggagtttatg agtttaaaaa taatcaattt gcggcttggt ttaattttaa caacagtacg
     7381 attgaaacta ttttccctga taatcctaat agtgcttatc tttatatgcg aatggatgga
     7441 ggagtttttg atacagaagg aaatgtttgg tttacaaatt cactcgtgtt aaatggaata
     7501 aaatttagaa aaccagatgg tacctggtca aagttaacct atccttcttt attatcaaaa
     7561 gaaacattag gagaattaat gatattaaaa caaaaaacgc aacagaaatg ggttttatca
     7621 gtacgttcag cgcctgggat ttgcattttt gacgacaatg gcacgttgga tgaccaaaat
     7681 gatgataaat ctatttttta ttcttctttc caaactcctg atgaaaaaat cactccaacc
     7741 tcttattact gtcttactca agataaaaac ggtgtcgttt gggtaggaac agatatggga
     7801 cctttgcttt tttataatcc caccaatgct tttaattctt ctgaatttat ggcttctcgc
     7861 gtaaaaattc ctcgcaacga tggtactaac cttgcagatt atctgcttgc agatgaaaag
     7921 gtgaaagcaa ttgctattga tggagccaat cggaaatggt taggaactga atcatcaggt
     7981 gtttatttaa tgtctgaaaa tggacaggaa actattcatc attttactac tttaaatagt
     8041 cctttattat ccaataccat tctttccatc gctattaatc ccgtaacagg agaagttttt
     8101 ttcggaacag gcaatggtat tatttcttat caaagtgacg ctgttgaagc tggtgatact
     8161 tttggcgatg tttatgctta ccccaatcca gtacgagaaa attataatgg cattattact
     8221 attactggat tggtagataa aacccacgtt aaaattactg atttaaatgg taatttgatt
     8281 tgtgaaacca tatcgaatgg cagcattgct acctgggatg gaaaagatgc gcacggacgc
     8341 agagtaagca ctggaatata tctaattata tgcaccaatt ctgaaggaag tcaaagtgca
     8401 attacaaaaa ttttagtaat aaattaaata aacgtaaaaa ataaaatact ttttgtgaat
     8461 tactcgaata aaagaaaaat ttaattgata actattttat taactgaatg atgctttaca
     8521 tgatgtcgtt agaaatgcat tgtttcatgt caataaataa caaggagtcc ttatgtaata
     8581 ttaaattaga acaacttttt ataattttaa aagatatcag aagtttttta attatttgat
     8641 aaatattttc ttaaaagttg tcatatcatt taaaatattt aagcattatc cggaacaatt
     8701 gcttgactaa tacttaaata aggctaattg ttttgtgata tcctcttacc tccttgtgtt
     8761 cattttctca ttattaaaat tatctgtatt agcatcgatt ttttgaaaaa atgaaaatca
     8821 aaaaaataaa tttcaatcga aattatgaat aatttaatca ccaaaaatta taaatataac
     8881 ggtaaaacat tacttctttg catcatatat cttattataa atggtttatt tatttttaaa
     8941 tatataagac gagttaattt tgttgattct cttatcgttt tttttattta tattttattt
     9001 atattaactt ttttcattat aaaccaaaaa ctccagaaaa aagataaact tttaaagttt
     9061 tttctgattt ctcttctcat aattagtttg gctttaatta tttttattca aataaaaatc
     9121 aatccattaa cacttaaagt tgatagatgg tcagcaatta ataattttct atctaactta
     9181 tttaaaggta aatcacctta ctctgctaac actcatttag gaggatatgg ttctccattc
     9241 cccgtttggc aaatttttca catacctttt tatttattag gagatgtggg actttgcatg
     9301 attttttgtt ttgtctgttt gattttatgt gtaaaatggt attctaattc aaacaaagta
     9361 acatttactt ttctttccct tatggcttta tctcctgctt tttggtatga agtctcagta
     9421 agaagcgatc taatgtataa tttttttgtg gtttttattg ctttactgat tatacaaaaa
     9481 aagaatttta atcttcaaaa caatacaatt agcttgggaa tcgtttgtgg cttatttatg
     9541 tctaccagat tatctgtatt tcttccttta ttcttatatt ttttttatga attctggaca
     9601 ataaatttaa aaaagaaaat actttttatt gtttcaggat tggtcacttt tattttatct
     9661 tttttcccct ttgttttatg ggattataaa actttatttt taactaatca taatccattt
     9721 atattacaaa caagacaagg ttttggaatt gatattataa ttataatagc attagcaata
     9781 tacttttcgt ttaaagcaaa aaataatttt tataaaatta tcattttcat ttctttctca
     9841 ttatttatct tagttgcaat taatttttta tttcgattgg cgaatgaaaa ttttgcagat
     9901 ttattgtttg actctggata tgacattacc tattttaata tgagtttacc ttttataata
     9961 gctggtataa gcttaattaa tagagctatg gatgctgatc tttaaggcta aatacaaatt
    10021 tatgtaaaaa taaacgattt cgaactgatt ttttatacca cagtatcttg tctgctggaa
    10081 atattccaac ttctcttatt gacaaacacc ttgatatttt tgaataaatg ctcttaaaaa
    10141 tgcaaaataa agttactcat actttgtcaa attgtccttt gttttatttg aaaaaacaat
    10201 ggtggatttt cctgattttt atcagtttat tcataaaaat ttttcttttg ccagtaaaaa
    10261 ccggtgatta tgttgtatat ttagaaccct ggattaattt tataaaaacg catggctact
    10321 tttctgcctt aaaatatgat ttttacaatt atactcctgc ttacttgtat attttagccg
    10381 caattgctaa aataggatta aatcctctgt ttcttatcaa aggaatttct gttttatttg
    10441 aatatgtaac agcttatttt gtggggaaaa tcgcatggtt aaaatttaaa gataatctgg
    10501 taattatcat tgcgttagca attgttcccc tgcttccttc tgtgatgctc aatagcagtt
    10561 atctctcgca atgtgatgct atttatgctt cgtttacagt agccagcatt tattatgcac
    10621 taaaaaacaa atcttttctt tcaattttaa tgttaggaat agctttttcg tttaaattac
    10681 aagctgtttt tatcctgcca ttcttttttg tgatgctctt acggggcaat gttaaatggt
    10741 atcatttttt tctcattcca actgtctatc ttattagcat ttttccagca tggttggttg
    10801 gtagaccttt ttcagaatta ataagcattt atttttctca agccgattat taccgattgc
    10861 ttaccatgaa tttccccaat ctttatatct ggttcaacaa taactattat gaattaattt
    10921 caaaagcagg gatttttttc acttttttat ttactttgtt tggtggaata aaattaagct
    10981 taaaaagaat taattttact tttgaaaact ggattcagct tgctttttta agcgtaacgg
    11041 taataccttt tttcttgcca ggtatgcacg aacgttatct ttatctggga gatgtgctgg
    11101 gagtagcgta tttaatgatt ttccctcgta aattttatct gccattagcc gtctgggtga
    11161 tttcgcttta ttcttatatt cgctgctctc gcttcaatga aattctacct atgtggccag
    11221 ctttctttat ttatttatta attttagtct ttatttccat tgatttttat ttaggaattc
    11281 gaacaaacag ttcattatca aaaacaaatg aaataatttt atgagaaaag gggaaatttt
    11341 ttacctttct tatcaaaaat tatcaaaaat tttttcatta tgtctttaaa aataaggaaa
    11401 cagtttcttt ttattttttt cattatcagt atttacactt tgctgacaat tgtatgctgg
    11461 aatcattgct acttctggga caatattcaa caaatttcaa aagaagcaca ttggttttat
    11521 caaaataatt tttcttccct aataattccc atacacagtt caagtgatga aattgttccc
    11581 acgggatatc atccaccttt gatggggata atgacggctg ccttatggaa aatttttggt
    11641 tatcatttgt gggtttctca cttttttgtt ttttgctggt tttttattct gctttatcaa
    11701 accataaaat tgctgcaact aattttccct caaaaatatg caagtggggc acttatcatc
    11761 gtacttttgg aagcaacagt gttgactcaa tttgctattg cttcgcccga ctttatttta
    11821 ttaactgcat ttgtgatttg tttacgagca attttagaac aaaaaaaaat tttactttct
    11881 gtaggtttga tttttttatg tggcattaac gccagaggct tgttaacagg aattgcattg
    11941 tttttcacaa attattatta tttatattta caatcagaga aaaaacatac ctttgagtgt
    12001 ttgaaaaaat cttttatccc ttttcttcct actcttctgt tattaatagc atattattgc
    12061 ttgtatttta aaaccaatgg atggtttttt gccgattctt cagaagcggg acattatgct
    12121 ttacctgcta atttttccat gataataaaa catctttttg aatttattat ccgactcata
    12181 gaaaacggac gtattgttgt ttggggaatt gctttttatt tgtttatact gattattcga
    12241 gggaaaatac aaaccagcat caaagataat gtattgattt tttgtttttt atttttaatg
    12301 gcagaatacc ttgttttggt ttttacaacg cgaatgcctt tttcaggaag atatttcatg
    12361 cccttgtttt ttgtgttgac tttaattact ctatcaggga ttactcattt tttcgatcgt
    12421 aaaaaaatta aaattacttt tgttgtaatt ctgctgttcg aattaactgg acatttctgg
    12481 atctatcctg aaaaaatagc tcaatcgtgg gatagtacgc tggctcattt gccttattat
    12541 gaattgagaa aaaattgttt cgattatatt gatcagcaag gaatagatta tcagaaaatt
    12601 tctgcaggtt tttgtttgta tggcgacaga ggttacatag aactccaaca tgcaggcaaa
    12661 aaagtaaatg atgaattcaa taatgagtat tttatttatt ccaatatttc taatatcgct
    12721 gatgaatggc tctttcagtt gaaagatacc actcagtgga aacctataca aacttttcag
    12781 aaatggccag ttaaaataat tttatatcaa aagattcaat aaagaaattc cttaaaattg
    12841 actatcctcg ccgcaagcag cgaggtatta cgccaatttt atggcttcgc caccggaatt
    12901 tctaaaaaaa taaaaacaaa agctaccccg acgcaagcat cggggaattc ttacgattaa
    12961 atttttcctg ctatgaacta ttctaaaatt gtgatacaat ttttcaaatt tggtatagtg
    13021 ggtattttaa acacactcat tactgcattc attatctttt tgttgctcaa ggtttttcat
    13081 tgttctgatt atgtttcaaa tattattggc tatctcgcag gtttgataaa tagttttatc
    13141 tggaacagaa aatggacctt tgaaagtcat ggaaaaattt cagtaacagt ttttaaattt
    13201 atcattactt ttgttatttc ctatttatta caattgggtg gcttacatct attgctccaa
    13261 tatacaacaa tagacaatta cgtttgtcag ttgatagcaa tggtgattta taccattatt
    13321 aattttttat taaacaagta ttatactttt aaaatctaat tcgaatgtat cagtcattaa
    13381 gcataatcgt tccttgctat aatgaagaag aagtaattat cgaatcttat aaaagattga
    13441 agcaagtttc gcaaaaatta gattttgata cggatatcat ttttatcaat gatggaagta
    13501 tcgaccgtac cgaacaaata ttatcagaaa tagcgaaaac ggataaaaat gtaaaagtaa
    13561 ttagcttttc aagaaatttc ggacatcaaa atgcagtgac ggcgggttta aatcattgta
    13621 cttctgattt ggccgtaatt attgatgccg atttacagga ccctcccgag ttaataccgc
    13681 aaatgataga aacactaaaa aaaaatcagg ctcaagtagt gtattgtgta cgtaaaattc
    13741 gaaaaggaga aagtttattt aaaaggtttt cagcaaaaat attttatcga tttctgaatt
    13801 ttttgtcaga aataaaactt cccgttgata caggtgattt cagattggtg gataaaaata
    13861 ttattaaaga attcaaccga atgagggaaa aaggaaaata tattcgtggt ttaattgcct
    13921 ggatgggatt taaacaagta cctttttatt acgagagaga agcccgtttt gctggggaaa
    13981 caaaatattc gctttctaaa atgattaaat tggcaactac ttccatgctg tatttctcta
    14041 aaaagccatt aaaaatagct atttatctcg gattttttac aacattactg gctatcttat
    14101 tggttttgtg gtcgatattt gggaaaatat ttggttttac aaatgctgat cctggatgga
    14161 catccttatt tatagccatc gtgttttttg gtggaattca attacttacg attggaattt
    14221 taggaactta tataggaacc ctttttgatg aagtgaaaga tagacctgaa tatgtagtga
    14281 aaaatctact gaattttgaa aataatatag aaaaggaaaa ataactacaa taagcttagc
    14341 tatttacaat tctcataatt caagcaaatt ccaccaatta atgcagcttt taaaggaaca
    14401 taagatgaaa aatatttttc cttttatttg ccctgatgaa tttcaattca atgaatttta
    14461 ctaagatcgt atggtgtttc ctgataaaca aagtaattaa gccagtttgt aaaaagcaaa
    14521 ttagcatgac ttctccagcg aacaacaggt tcattttctg gattatcatt caaatagtaa
    14581 tttttaggca tctttattgg caaccctttt gctaaatctc ttttatattc gtcatccagt
    14641 gttcgagctg aatattcaga atgtccagta ataaagaact ctcgtccatt tctccccatc
    14701 accatgtaaa cacctgattc tgcggattct gaaagtaaag taagctcggg aactttctta
    14761 atatcttcgg cacgaacttc agtgtaacga ctatgaggaa cataaaatac atcgtcaaaa
    14821 cctcgaaaaa ttgggttttg tggattatat agcgtatgtt cgaaaacgcc aaacattttt
    14881 tctttcaatg gatatttagg aatgccgtaa taataataca aaccagcctg ggctgcccaa
    14941 catacaaaca tagtagaagt gacatgcgta cgcacccaat cgaaaatttt cgtgatttca
    15001 tcccagtaag tcacttcctc ataagataaa tgttcgatgg gcgcacctgt aataatcatt
    15061 ccatcataat tgctctcctt gatgacatca aattttttgt aaaaagccat catgtgctca
    15121 attggcgtat ttttgggggt atgactttcc aattgcaaaa aatcgacttc aatttgaagc
    15181 ggtgaattcg aaagcaaccg tatcaaatca gtttcagtcg taatttttaa aggcatcaag
    15241 tttaatatca caatcttaag aggacgaata tcttgatgag aagctctatc agaatccatt
    15301 acaaaaatgt tttcttttct gagaatttcc acagcgggca tcgaggcagg aatatttaat
    15361 ggcatatttt tatttttttc aaattttttt attcaaataa gctgattcgt aattgtttga
    15421 ttattaaaac aaccttacaa aaacaaagtt caaatttaat aatatttacg ataaattttc
    15481 cagtaagctt cacttccaat aaaatcattc agaaaatcat ttaatttttt ctgcaattgt
    15541 tttgattctt tatacaacat ccaaccatat tgctggtcaa aaccgactgg taaagaaata
    15601 tcgaggtttg gatattgtaa tttccatttt aatgcatctc tttcatcaca aatagtttgt
    15661 ttgattttac ccatagctac ccattttact aaatcttcac tattgacatc atttacttca
    15721 accagataaa ttgtatcagc aatttcatca gaaagattct ttaatcgtag tttatgaggt
    15781 gaattttttg gtatataaat ggtgtcgcca gctaaatcca cttgattttt aataattttt
    15841 tcagatgaat ccttgcattg tatcaatact tgcctggaag ttttaagagg aatagtaaac
    15901 aaatattcat tttgccattc ggaagtaata ggaataaaat cagcataaat atcataattg
    15961 cctttcctta aaccttccaa acattcttta aaattattat tagtactaat tattaattca
    16021 agattcaaac tatcagcaaa agcttttgca atttcatatt gaaatccaaa aacactgtct
    16081 cctttcattg caaaaccaag agcactgctg tcagtgacaa cagacaatct tcccgatttt
    16141 aaaatagctg gcaaattatg tgctgtcttt tctctgaaaa acaacagttt aataaataaa
    16201 attaataata caaaagcaat tgtaaagaaa atatactttt taattttcat aaataagtaa
    16261 ctattttaaa tgaataaatc aatcgaaagt tttaaaaata ctggtttctc cttttaatta
    16321 taaaaattgg tagaaacccc aattttaaaa attgctggag aaaggggata attaggcata
    16381 gaaaaataag cagttttcat aaacaactga ttgatatgat aatattctac aaaaaaacga
    16441 gcttgtttca aatgaaagtt ggcatataca ttaattactg gaaaatttcc tacttttgtt
    16501 gtggactgat tataaaattg tgctgtagct ggcatataac aaggtgaata atattctgta
    16561 aaataacgta aattagcgcc taattgagca cttagcacgt tgaaccacaa gccattataa
    16621 tataaattat gatacaaggc aaatgcagga agtggaatta cttccgaatt tgaacttcgc
    16681 tgataaacaa tattgttctc caaattaaat tttccaaaat gaaaatcctt ttttaaattc
    16741 aatgccagaa cttgtacatt tccattgtgt tggaccggca atgcttgaga attaaaataa
    16801 atataatcag tcaaattttc aacagaaaca ttgagtgaaa aatagcgagt tggtatggaa
    16861 aaggttccat taatattggt acggtaggtt ttattaaaat tattatccca gcaaaaatgg
    16921 ttggaatgat aatgttgcaa aaaataagag ggttcatcac tctttacaaa tccacccgcc
    16981 gttaaaataa tactgtcttt aaacaattta aaatagccac ccaatttacc tttgatttga
    17041 aagtctccag ttttatatcc cagcaatccc atttcgccta aaatttcata acgaaaacgt
    17101 ggtcctttgt attttgaaag aattccgcca acttttgtac tcgacatatt ttttcgctct
    17161 aaaactgaat caattttaaa agtatatctt tgaacttcat tctgtaaaaa agcagtcatt
    17221 ccaaacttca accatttatt aaactcctca gccatattta ccgatacagt attggtcaac
    17281 gattgcaaag cagccgtatc attggtttgt gtaaaaggca gataagtatt ctggtaaaaa
    17341 tctttttcta ctgtaggttc ataatatctt tttcgaggat cttcatattt tatagtatgg
    17401 gcaaatcgcg taaccgggac atattccatt tttactgaat ctggtgaaat atgaatgggt
    17461 cgttcaaatc caattgtgta ttgttgatta taaaaaaaga cattctttct ataaactgaa
    17521 tatccattaa ttcgagtagg aatatctttg gtagctattc ctaagggttc atatatataa
    17581 gaagaatcgg caatccctcc attttcatga ttgaacatat tgtttacagt cactaagcca
    17641 gtagcactat aatgtttacc attataacta ccaaacaatg agccgctaaa gcgttttgct
    17701 gcttgattat tatattcgcc gaccgcatag aaataatcca ggtttgtccc aaaattcagt
    17761 gatttattgg cattggcagt aaaaaggaat tttatatgat cttgttggcg atatgtggat
    17821 ccaccggtat tgtaaacaat atgagtataa ggcattttta cattataaaa agttgccttt
    17881 tctatatcca ttatgtaagg ataaaaagct tctgaaaaaa taaaatcgct tttttcagga
    17941 cgatcaaaat acagttttgg ttcaatggga gaacccaagt tcccattata tgaattggca
    18001 atactgaaac gatcaatggg attagcatcc tgaaaattaa ggtgtaaagt atctaccgga
    18061 atcgtatcta ccacagcaaa aggatcagaa attttccatg tctttaccgg acgtggtttt
    18121 acaggaattg ttccccaaat actattagta aagaaaaaaa gaatacaaaa aacaagatgc
    18181 ttttttatag gcattaaaat gatagttaag aaattacctg ccaaaaattt aaaacgatag
    18241 caaaattaca aaaaatatcc cttatgaata atca
//
LOCUS       DAOSAS010000022        17418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_2257_length_17418_cov_8.568220, whole genome shotgun sequence.
ACCESSION   DAOSAS010000022 DAOSAS010000000
VERSION     DAOSAS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17418
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2257_length_17418_cov_8.568220"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4323
                     /locus_tag="PLR72_01930"
     CDS             <1..4323
                     /locus_tag="PLR72_01930"
                     /inference="COORDINATES: protein motif:HMM:NF036966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOK36003.1"
                     /translation="HDNWSNLYIHENVRQKPLPTNKWWTNYVFSQYGGDAWAYPHAVS
                     ADNEGINIKIPSGFQGGGMITTPFLEIKGASHLAANEEKIVFADFESTTYPEGWTVGP
                     NPPYPGPVSLNDISQTPTPTGFTGNRFIDSYKGNEAKLTLISPSFVITKNYIHLRVGG
                     GNYINDTYVGLFINGVRVFAETGQNSGTLTQRTWDVSSYIGQTAEIRIVDNSTGGWGF
                     IMCDEIIFSDSNFTGSGYENDFSPKNSNVYDWTDLGFTFRSEDQDGRYMDVTLVHGIP
                     FTWLELNNLVPLLIPGATCRIYNLSGEEVTNFPATLNAFTMEFGNRIYGVHLPSGSVL
                     YRSSGGDFQVEIPTSSPKYLVISDLPNRNLLSLYDAYARNKPVNTSFLWDYKISEGKI
                     NTTFQIDTKNLETGALNGETLMSFLPHHYRNTTTNFNFISGGDYQLILGKMHTASGRV
                     FSIDYPFGGMPPYLPAPLNLTATQKNRLNTMLNYKADHSGGFNGNTYAKGLGENSNVM
                     LMAKTMNANSAFTTLKNNLKTELTDWWTYNPSEASNKKYFFANYPDYGAIIGFPPGYG
                     SQGFNDLHFHYGYFTVGAARLMMVDNDFKQNYADMAKLVVKSFANWKHYPEGNDYLPF
                     LRTFDPYFGHSFAGGTGDGGGNNQESTSEAIHSWFGIFLLGIELNDPELYKLGAMGYL
                     LETTAAKEYWLDIYNENFPTTYGKDYVGILRTDNLAWATYFSGDAAWILGIQAVPCDF
                     FYNYLAKDSVSMKSIWQSMIVDRTNQMIDPDGDGPLGLQPLSTTTDPFQNIYEMGSYL
                     GGYHLNIMNTFDPVKAAQFTDSLYSKGGSWATDVNMITDYYITNATITYGVPAEGFHT
                     SIPSGAVYKNQKGELTYLLYNPTNKEMDVNIYKDNTIIETIKVAAHSYYNSRVVSGQK
                     PVVTFIKPAMNDKFALNEKVKLTVSATDIDGKVQWVDFYEGKAKIGSVVQEPYTIIYT
                     PTSAGVKEICAVAVDDMGNRSDSCKIKIEILPIQQTPYKGTPWNVPADKILAVQFDNG
                     GPEIACHDNELAIQGGNNLRSDTGVETENSNGGDGNIGYTNAGEWYEYTINVQTAGIY
                     SLSVRLSSAYGGALRFEFDGEDKTGSININKTGGWGNYKDTIITQIPLKAGIQVMRVI
                     IDKTGANLSSYKFTLINTNMPPAAYAGNDIIISYPQNSVQLSGLGEAYGGATISSYEW
                     KQIDSNPIINIFNPFSPTTTVSGLNIGTYVFELKVTDSNGLSGTDQVAVLVKPENYPP
                     ISVPGNNFTIKYNQQPVLLDGSNSSDPDGIIVKYQWEQIDNNNRLTIEQSSFESPFAS
                     VSGFEPDKWYILKLIVTDDAGASSSQNICIFVESTSEVGGLSNLPLLIYPNPFTDHLK
                     IAFNKEAGFKRLLLRSISGNLLIAKNINSSYEVNLNTSELKSGCYLLTLISDQQVITY
                     KLIK"
     gene            4357..>6125
                     /locus_tag="PLR72_01935"
     CDS             4357..>6125
                     /locus_tag="PLR72_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOK36004.1"
                     /translation="MNKKLLFIVLTLCTIQFGFSQTLISQGKTAWASSSEGGYVAGNA
                     VDGNLDTRWGSNWSTDPNPADAWWAVDLGDEYYISRVEIQWENAYAKEYKIQISNDKN
                     FSTFTDIAYVNNGDGGLDVVPTNINVKGRYVRIKCITRATGYGYSFFECRIYGTKTLT
                     PVSITLDVPYVQYLKLGLSPEDLNGQNEVIVQNTNQTIQLLYNEGSPLTVSLQDFRGN
                     FDVNFWTLKSGSTTDTIKGVPLTVYVYSGMVIHVKLTPKPKVGNIPPVADVGPDQIIY
                     LPQNGIQLDGSNSTDPDGEIISYKWEQVSGPSTATMIDIFTPIVKINDLVLGDYKFRL
                     TVMDDSLATGIDEVVVSVLPPAQVDFHLQTPTDGSMVTTTRKPTLSWETCSGASKYEI
                     YLNITRSDYEWYASGNLLDRYTKIGETTSTSFTVPTELVDRWTYKWYVIATTSSGNKY
                     SNKSQFGLYIPYLEQENDGVNIVNGCRDMNKNGTIEPFEDWHLTPEERLNDIMSRLTT
                     EEKVCQLFYDGNNNPLDGFAFSYGVEGGMRTAQYAASRTRMGIPIAFTGDKIHGWKTI
                     YPTQLGLAATRDMDLVYQCGNLQR"
     assembly_gap    6126..6225
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6226..7917
                     /locus_tag="PLR72_01940"
     CDS             <6226..7917
                     /locus_tag="PLR72_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOK36005.1"
                     /translation="YHMKPWHAAVEANAASVMPGYNTSPLIDPLNGANTSKKVIDYLR
                     NEIKFKGFVVTDWLAANTEQSTKSLGAGIDVMGGAPSANTDINQLVQNIGMDRINEAC
                     RRVLDMKIRLGMFENPYSDPTCTWTKEDHHAIVLNAAKKSITLLKNDGVLPLKLNSGD
                     QVVVAGPRATWINKDNDPNTIWQSIYYDDPQAKTYLQAITERGAQDGLNVYQDDASNP
                     KVAVVVIGEKSYTHGTEWADKNPNIPDDQLSIIQNFHNRGIKVVTVILMPRPYVLTPL
                     LELSNAILVVYRGGTGIGQATAACIFGDYAPSGKLPFQLPKSDTQIGTDNLNNMVEKW
                     DLPYDIGATDAERILIRNYINQDLTVPPIFGDPLFQYGFGLQGFGNSDDSSPLAFDLL
                     SPSNNSIFQSNQITFSWQESSDPESSIAYYEVYIDDVKRAEVTSPFYTTIVSDCGIHS
                     WYVVAVNGSGLRKNSTSTYQFTISPSGLATAGMNPEVSVFPNPSNGHFVIKLNNFVEK
                     YYIKITDMTGKIIYANDSPSSINELDLTKYPQGVYFMCIECNKSQFRKVIKIIKI"
     gene            8011..9750
                     /locus_tag="PLR72_01945"
     CDS             8011..9750
                     /locus_tag="PLR72_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOK36006.1"
                     /translation="MSKSVLLLAALFFFYPANSKGFLSVKGQRIVDESGHEFLLRGMG
                     LGGWMLQEGYMLQMPGGGNQHQIKAKITDLIGDEACERFYEKWLSNHVTKTDIDSLAK
                     WGFNSVRLPMHYNLFTLPIEEEPDPYKNTWLSKGFELTDSLVSWCKANKMYVILDLHA
                     APGGQGKDANISDYDPTKPSLWESDLNKNKTIALWQRIAEHYKDEPNIGGYDLLNEPN
                     WTFENKDIHGREDLLNKDIWDLYIEISKAIRKVDKKHILFIEGNGWANNFNGFPGKWD
                     NNMALSFHKYWNPNTQQSIEPFIQLRKKYDIPLWLGESGENSNKWFTDCIRLVEKNHI
                     GWSWWPLKKVSSVVNPFTIIAPSGYDQLLNYWKYGESKPSKNFATQVLFELTDNLKAE
                     NCSYNKGIIDAMFRQRFETSTLPYTKNFIPGRIYGVDYDLGYYNRAYFDKDNENIMVN
                     GKRSVGNRGNQYRNDGVDIIRCNDKNEYTNGYHLFAIEKGEWLKYTVDVKEKGDYQIF
                     ARIKPTSYPVKFNFIFGKKKKKIEFSHSDINPNDWQTIYCGNINLKKGKQNLFISFES
                     NDFEFNFLEFIKK"
     gene            10015..10908
                     /locus_tag="PLR72_01950"
     CDS             10015..10908
                     /locus_tag="PLR72_01950"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK36007.1"
                     /translation="MKKLFTKKALMLLCAFFMASALFSQQKRYVFVLGGNPTWNSSWI
                     NLRLDDVNRVLYVWPDGTSLSGTTPVGTGSLGQDGYIAFTVGNIGWWGCGYFVLPTSQ
                     MDLTDVTEDWTFHFAIRTDCAQDITVGLNGSSPDPSDPFITDKTEGKVILNRTNLPLA
                     QRDKTQWVEFNIPMSQLMSGGSNNLKYLAPLTDQNYLFFSGGNDAGSFIAWDNVYIGA
                     PDGGSGIFTPKAEKLAFSIDDDGNYLNVFNNNSNDEINIYSISGAHVLNSKVNRVNIS
                     NLSSGVYIVKSGNLVNKFTKK"
     gene            11267..14362
                     /locus_tag="PLR72_01955"
     CDS             11267..14362
                     /locus_tag="PLR72_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK36008.1"
                     /translation="MKKKITLLFLWVTLVMNVFSQTFNVSGTVLSAEDNQPIIGANIW
                     IKGVNKGTVSDINGRFSIEVEKNQTLVISYLGFENVEQRITGPVSNLKIILQPSTVVL
                     EEVVAVGYGTMKKRDLTGAIASVTGEDLRKLPAATVDKALQGLSPGVTAIANSGQPGA
                     DMVIRIRGIGTINDSSPIFVVDGVQVGNINFLSPADIKSVEILKDASACAIYGARGAN
                     GVILITTRQGDKSGKMKLNVEAYYGIQNKAKKLDLMNSEQLSRFLGYTGDTSEEFNQW
                     IYDNFAGSKTYIPKGIDFSSYDTDWQEVVFDDNAPIQNYYVAAEGGSEKTNYFLSAGY
                     FDQKGIIMASFYNRFTFRINSSTKINDWLTLGENLSLMNSHNRNAPNNNDNYSMLNSA
                     LRFAPWDPVRFPNGNVSASTIANYPNPLSMVEFIHPKNNWDRLVGDAYLQIKPIKELV
                     YKADFGFDLSYGQHREFKDKYEVAPFDKMDNNFVTSSFERYQTWSIEQTLTYSKILEK
                     HNFSLMIGQSLSEYSYYSLGGSRTDIANPRKENWYISMGTGEYTVSDGVGRSRLASFL
                     GRLNYSYADRYLLTVNFRADGSSKFPKDKLWGYFPSFSLGWRVSEENFFIPLRSVFDS
                     FKLRVGWGQIGNENIPSNNYFAKVQTGSQFVTYVLGDDQHMAYGATMTSLPSEYLKWE
                     TTEQTNVGIDVSFLNTRLNGSIDYYIRDTKDMFLNVVLPKHIGMLFSSPANVGKVRNK
                     GFEFLVNWKDKVSKDFNYSISANLSTVNNKFVDMSMSSPLYYEGFKGENLVVCKEGYP
                     LYSFYGYTYDGIFQNQQEVDEYVKRDENGDPLLDANGDLQYIISGVQPGDVRYVDKNN
                     DGQITDLDKSIIGSSFPTFTYGFNIFVGYKGFDCSLFFQGVSGNKIYNCNKFFMQGGE
                     GIKLTNLGTEMLHSFTTDQPSTTIPRAKGRASNYWASDRFLEDGSYLRLKNAQIGYSL
                     PPKVLSNAKIESCRLYISGANLLTFTNYTGYDPEASFRGVDRGNYPQAVTFLFGVQLG
                     L"
     gene            14396..16024
                     /locus_tag="PLR72_01960"
     CDS             14396..16024
                     /locus_tag="PLR72_01960"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOK36009.1"
                     /translation="MKKIIYLFFFVVSGCLMGCTDFLTPEPLATEKLSDFFNNDSAAI
                     QAVNAAYVPLQWQLGGTYANEWFIGDVVSDDALKGGASLYDMQVVGLMENFQTTANNE
                     YLDTFYDINYQGIFRCNFAMENINGMTADKWSDQHLKTRLIGEAKFLRALYHFRLMRV
                     FGGIIKGDRTFNQHDTQIARSSKAEIVRFIRQDLEDAIKALPLKSEYPASELGRATKG
                     AAQALLMRVILFDILNSENPFGDYDRVMSLGDSIIASKEYRLEDNYADIFKVFESPTE
                     ARFEKENGPESVFEIQYMEEATSDYGGLGRTRGNFDPIMTRTRANNSGWGFNRPTQDL
                     YDEFEPDDIRRDEAILPFDAGDVYAPGSYDNGYHARKAALGFWRTVGGKQVFYMPPLK
                     HPTRAPNNTIVIRYADVILMYAEAACEINNIKEGKDKLNLIRLRARNSASDPTNPDIL
                     PDFPYGSYVENQVDLRKAIRHERRVEFAMEGHRWFDLIRWGIAGDVMNAYREKYIAKE
                     GKDMSPFIKGKHELFPIPETQLNANPLLQQNPGY"
     gene            16045..17088
                     /locus_tag="PLR72_01965"
     CDS             16045..17088
                     /locus_tag="PLR72_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOK36010.1"
                     /translation="MSKKSRKLFKIILILLLFIIIPVKSQNWQLVWSDEFNGTTLNTD
                     NWNIVEGGSGFGNQEKQFYTSRPQNLKVENGNLVITALLENYTVGSMTWNYTSARINT
                     SRKKDFTYGKIEARIKLPTGRGTWPAFWMMGYGSWPSCGEIDILEFVGRQPDRFQSNV
                     HTKDYNGMNGQNFHIEKVYPGLPNDFHVYSIEWTSEKIKFFFDGAQYWTFSSLSVDPA
                     NYPFNNPFYIILNLAIGGTMGGAVDNSIFPQKLLVDYVRVYRDINAVNDPSSKQQPVV
                     STLVSDNLNIQIPENQRNNKIIIYDLNGKTIRQQNTSERELNINVSDLTKGVYIVKIV
                     SDENIYTSKIIKQ"
     gene            17085..>17418
                     /locus_tag="PLR72_01970"
     CDS             17085..>17418
                     /locus_tag="PLR72_01970"
                     /inference="COORDINATES: protein motif:HMM:NF023218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOK36011.1"
                     /translation="MIRNILHFNIKRKVFSFTIILIFFCISDLCAQKRSTKRGLAYSI
                     PYVEDLVPLSRGISWFYNWGIFPSSAIDNYYSNYMEYYPMAWNGVNADGLRAFKAKHP
                     ECQYLLAFN"
BASE COUNT         5653 a         2485 c         3423 g         5757 t
ORIGIN      
        1 catgacaact ggagcaattt gtatattcat gaaaatgttc gtcaaaaacc gttaccaacc
       61 aataaatggt ggacaaatta tgttttttca cagtatggtg gcgacgcttg ggcttatcct
      121 catgcagtat cagcagataa tgaagggatt aatataaaaa ttcctagtgg ttttcaaggt
      181 ggtggtatga ttactactcc ttttctcgaa ataaaaggag cttctcatct tgctgccaat
      241 gaggagaaaa tagtttttgc tgattttgaa agtactactt atcctgaagg gtggacagta
      301 ggacctaatc ctccttatcc aggacctgtt agtttgaatg acatttctca aacacctaca
      361 cctactggtt ttacaggtaa tcgttttatt gattcataca aaggaaatga agctaaatta
      421 actttgattt cccccagttt tgttattaca aaaaactata ttcacttgcg tgttggaggc
      481 gggaattata ttaatgatac ttatgtgggt ttatttatta atggggtacg tgtgtttgca
      541 gaaacaggac aaaatagcgg aaccttaacc caaaggacat gggatgtgtc aagttatatt
      601 ggacaaacag cagaaattcg catcgtggat aattcaactg gtggttgggg atttattatg
      661 tgtgatgaaa ttatattttc agatagtaat tttactggca gtggctatga gaatgatttt
      721 tcgcccaaaa attcaaatgt ttatgattgg acagatttgg gtttcacttt cagaagcgaa
      781 gaccaggatg gtcgctatat ggatgtaaca ttagttcatg gtattccttt tacatggctt
      841 gaattaaata atttggttcc tttattaatt ccgggtgcaa cttgtagaat atataatctt
      901 tcaggagagg aagtaacaaa ttttcctgca actttaaatg cttttacaat ggagtttggg
      961 aatcgaattt atggtgtcca tcttccttca ggaagtgttt tatatcgatc aagtggcgga
     1021 gattttcaag tggaaattcc aacttcaagt ccaaaatatc ttgttatttc tgatttacct
     1081 aatcgtaatt tactttctct ttatgatgct tatgccagaa ataaaccggt aaatacttct
     1141 tttttatggg attataaaat ttctgagggg aaaatcaata caacttttca aatagatact
     1201 aaaaatctgg aaacaggggc tttgaatggc gaaacgttga tgagtttttt gcctcatcat
     1261 tatagaaata ctaccaccaa ttttaacttt attagtgggg gtgattacca attaattttg
     1321 gggaaaatgc atacagcttc aggtagggtc ttttcgattg attatccttt tggtggaatg
     1381 ccaccttact tacctgcacc cttaaattta actgctactc agaagaacag gctgaatact
     1441 atgttgaatt ataaggcaga tcattcaggt ggattcaatg gaaatactta tgcaaaagga
     1501 ttgggagaaa attcaaatgt aatgttgatg gcgaaaacta tgaatgcaaa ttctgctttt
     1561 acaacgttga aaaacaatct taaaacagaa ttaactgatt ggtggacata taatccatca
     1621 gaggcgagca ataaaaaata cttttttgcc aattatcctg attatggggc aattattgga
     1681 tttccgccag gatatggctc ccagggattt aatgatttac attttcatta tggatatttt
     1741 acagtaggcg ctgcacgctt aatgatggtt gacaatgatt ttaagcaaaa ttatgcagat
     1801 atggctaaat tggttgttaa atcgtttgcc aattggaaac attaccctga ggggaatgat
     1861 tatttacctt ttttgcgaac ttttgatccc tattttggac attcttttgc tggtggaaca
     1921 ggtgatggag gaggaaataa tcaagaatct acttctgaag ccattcattc atggtttgga
     1981 attttcttgt taggaattga attaaatgat cctgaactct ataaattagg agcaatgggt
     2041 tacttattgg aaacgactgc agcaaaagaa tattggctgg acatttataa tgaaaatttt
     2101 ccaaccactt acgggaaaga ttatgttgga attttaagga cggataatct ggcttgggct
     2161 acttatttta gtggagatgc agcctggata ttaggcattc aagctgttcc atgcgacttc
     2221 ttttataatt atcttgccaa ggattcagta agtatgaaat ccatctggca atcgatgata
     2281 gtagatagaa ccaaccagat gattgatcca gatggtgatg ggccattagg gttgcaacca
     2341 ctttctacaa ctaccgatcc ttttcagaat atttatgaaa tgggatccta tcttggtggt
     2401 tatcacttaa acataatgaa cactttcgat cctgttaaag ctgcccaatt taccgattca
     2461 ctttattcta aaggaggctc gtgggcaact gatgtaaata tgattaccga ttattatatt
     2521 accaatgcta ctatcacgta tggagtacca gcagaaggat ttcataccag cattccgtcg
     2581 ggtgcagttt ataaaaatca aaaaggggaa cttacttatt tattatataa tccaactaat
     2641 aaagagatgg atgtaaatat ttataaggat aatacgataa ttgaaaccat aaaagtagca
     2701 gctcatagtt attacaattc tagagtagta agcgggcaaa aaccagtggt gacttttatc
     2761 aaaccagcta tgaacgataa atttgctttg aatgaaaaag ttaaattaac cgtttccgct
     2821 acagatattg atgggaaagt acaatgggtt gatttctatg agggaaaagc aaaaattgga
     2881 agcgttgtac aagagcctta tacaattatt tatactccta cttcagcagg agtgaaagaa
     2941 atatgtgctg tagcagttga tgacatgggt aatagaagtg attcttgtaa aattaaaatt
     3001 gaaattcttc ctattcaaca aacaccttat aagggtactc cctggaatgt tcctgcagat
     3061 aaaattttgg ctgttcaatt tgataatgga ggacctgaaa ttgcttgtca tgataatgag
     3121 ttagctatcc agggaggtaa taatttaaga tcagatacag gagttgaaac cgaaaacagt
     3181 aatgggggtg atggtaacat tggctacacc aatgccggtg aatggtatga atatactatt
     3241 aacgttcaaa cggctggaat ctactcttta tcggtaagat tatcttcagc ctatggtgga
     3301 gctttacgtt ttgaatttga tggagaggat aaaactggtt caattaatat aaataaaacg
     3361 ggaggatggg gaaattataa ggatactata ataactcaaa ttcctttgaa ggctggcatt
     3421 caagtcatgc gtgttataat agataaaaca ggtgctaatt taagttctta caaatttaca
     3481 ctcataaata ctaatatgcc tcctgcagct tatgcaggta atgatataat tatcagttat
     3541 cctcaaaatt ccgttcaact gtctggttta ggtgaggcat acggtggtgc tactatttca
     3601 agttatgaat ggaaacaaat tgactccaat cctatcataa atatttttaa tccttttagt
     3661 ccaaccacta cagtttcggg cttaaatatt ggtacttatg tgtttgaatt aaaagtaact
     3721 gattctaatg ggctttcggg taccgatcaa gtagccgttt tggttaaacc tgaaaattat
     3781 cctccaattt ctgttcccgg caataatttt accataaaat ataatcagca acctgtttta
     3841 ttagatggga gcaattccag tgatccggat ggaataattg taaaatatca atgggaacaa
     3901 atagataata ataatcgatt aactattgag caatcttctt ttgaaagtcc ttttgcttct
     3961 gtttcaggtt ttgaacctga taagtggtat attttaaaat taattgtaac ggatgatgcc
     4021 ggcgctagtt cttctcaaaa tatttgcatt tttgttgagt caacatccga agttggagga
     4081 ttgtccaatt tacccttgct tatttatcct aatccattta ctgatcacct aaaaatagca
     4141 tttaataaag aggcggggtt taaaagatta ttgctaagat caatttctgg taatcttctg
     4201 atagctaaaa atataaatag cagttatgaa gttaatttga atacttctga attaaagtca
     4261 ggatgctatc ttcttacttt aatatccgat cagcaagtga taacatataa attaataaaa
     4321 taaattaata aaataaagtt gaataattta aaagatatga ataaaaaatt attgtttata
     4381 gtgcttacct tgtgcactat tcaatttggg ttttcacaga cattaattag tcagggaaaa
     4441 acagcgtggg catcttccag tgaaggaggt tatgtagctg ggaatgctgt tgatggaaat
     4501 ttagatactc gttggggaag taattggagt accgatccca atccagctga tgcatggtgg
     4561 gcagttgatt tgggagatga atattatata tcaagagtag aaatacaatg ggagaatgct
     4621 tatgccaaag agtataaaat tcagatttcc aatgataaga atttttcaac ttttactgac
     4681 attgcttatg taaacaatgg cgatggtggg ttggatgtag ttcctacaaa tattaatgtt
     4741 aagggaagat atgtacgaat taaatgtatt actcgagcaa ctggatacgg ttattctttt
     4801 tttgagtgta ggatttatgg cacaaagact ttaaccccag tttcaattac tttggatgtt
     4861 ccttatgtcc aatacttaaa attaggtttg agtcctgaag atttaaatgg gcaaaatgaa
     4921 gtaatcgtac agaataccaa tcaaactatt caattgttat ataatgaagg tagtccattg
     4981 acagtttctt tacaagattt tagagggaac tttgatgtaa atttttggac tttaaaatct
     5041 ggaagcacta cggataccat taaaggtgtt cctttaacag tatatgttta ttcagggatg
     5101 gtaatacatg taaaacttac tccgaagccc aaagtaggta atattccccc tgtagcagat
     5161 gtaggtcctg atcaaataat ttatttgcca caaaacggta ttcagttgga tggttcaaac
     5221 agtaccgacc cagatggaga aattatttcc tataaatggg aacaagttag tggaccttct
     5281 actgctacaa tgatcgatat ttttactcca attgtaaaaa taaatgattt ggttttaggt
     5341 gattataaat ttagattaac agttatggat gattctttgg caactgggat agacgaggtg
     5401 gtagtatcag tgttaccgcc agcacaagtt gattttcact tacaaactcc tactgatggt
     5461 tcaatggtta ccactacaag aaagccaact ttaagttggg aaacttgctc gggagcttca
     5521 aaatatgaaa tatatttaaa tattaccaga agcgattatg aatggtatgc ttctgggaat
     5581 ttgttagatc gttatacaaa aatcggtgaa accacttcta cttcttttac ggttcctaca
     5641 gaattagtcg atcgttggac ttataaatgg tatgttatag ctactacttc gtcaggaaat
     5701 aaatattcca ataagtctca atttggtctg tacatacctt atttagaaca ggaaaatgat
     5761 ggtgttaata tagtaaatgg gtgtcgtgat atgaataaaa atggcactat tgaacctttt
     5821 gaagattggc atctaactcc tgaagaaaga ttaaatgaca ttatgagtcg tttgaccacc
     5881 gaagaaaaag tatgtcaact tttttatgat ggcaataata atcctttgga tggttttgct
     5941 ttttcttatg gtgttgaagg gggaatgcga actgctcaat atgcagcttc aagaacacgg
     6001 atggggatac caattgcttt cacaggcgat aagatccatg gttggaaaac tatttatcct
     6061 actcagttgg gattagcagc gactcgtgat atggatttgg tttatcaatg tggtaatctt
     6121 cagcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntatca catgaaaccc
     6241 tggcatgctg ccgttgaagc caatgcagct tccgtgatgc cgggttataa cacttctccg
     6301 ctgattgatc ctttaaatgg ggcaaatacc agtaaaaaag tgattgatta cctgcgtaac
     6361 gagataaaat ttaaaggctt tgtggtaacc gattggttgg ctgccaatac tgaacagtca
     6421 accaaaagtt tgggagcagg aattgatgta atggggggtg caccttcagc aaatacggac
     6481 atcaatcagt tggttcaaaa tattggtatg gatcgtatca atgaggcatg cagaagagtt
     6541 ttagatatga aaattcggtt aggaatgttt gaaaatccat attcagatcc tacctgtacc
     6601 tggaccaaag aagaccatca tgccattgtt ctaaatgcag ctaaaaaatc gataacttta
     6661 cttaagaacg atggtgtttt accattaaag ttaaattctg gagatcaagt tgttgtagct
     6721 ggtccacgag caacatggat aaataaagat aatgatccaa acactatttg gcaatcgatt
     6781 tactatgatg atcctcaagc caagacatat ttacaggcta tcaccgaacg tggagcacaa
     6841 gatggcttaa atgtttatca agatgatgca tcaaatccaa aagttgctgt ggttgttata
     6901 ggagaaaaga gttataccca tggtacagaa tgggcagata aaaatccaaa tattccagac
     6961 gatcaattat ccataattca gaattttcat aatagaggaa taaaagttgt tactgttatt
     7021 ttaatgccac gtccgtatgt attaactcca ttattagaac tatccaatgc aattttagta
     7081 gtttatcggg gcggtactgg tatagggcaa gcaacggcag catgtatttt tggcgattat
     7141 gctccaagtg ggaagttacc ttttcagctt ccaaagtcgg atactcaaat aggtactgat
     7201 aatcttaaca atatggttga gaaatgggat ttaccttatg acattggagc aacggatgca
     7261 gagcgtattt taataagaaa ttatatcaat caagatttaa cggttcctcc tatttttgga
     7321 gatccgctct tccagtatgg ttttggtttg cagggatttg gaaatagtga tgatagttca
     7381 cctttagctt ttgatttgct ttctccgtcg aataatagta tttttcaatc gaatcagata
     7441 actttttcat ggcaggagag ttctgatcct gaatcttcta ttgcttatta tgaagtttat
     7501 atagatgatg tgaagcgggc tgaggtaacg tctccttttt atactactat tgtttctgat
     7561 tgtggaatcc atagttggta tgttgtggca gttaatggaa gtgggctaag gaaaaattct
     7621 acatcaactt accagtttac tatttctcct tctgggttgg cgactgcagg gatgaatcct
     7681 gaagtatctg tttttcccaa tccttcaaat ggacattttg ttattaagtt gaataatttc
     7741 gtagaaaaat attatatcaa aatcaccgat atgacaggaa aaattattta tgcaaatgat
     7801 tctccttctt ctattaatga gttagacctt actaaatatc cgcagggagt atattttatg
     7861 tgcattgaat gtaataaaag ccaattcagg aaggtgatca aaattataaa aatataggaa
     7921 aacatatgaa gagctatcag ttctcagaaa aataggttac aaaagagtga agaatttttt
     7981 taaaattttg tgatgataaa tagcttaaaa ttgtctaagt cggttctttt actggctgct
     8041 cttttcttct tctatccagc aaattcaaag ggttttttaa gtgtaaaagg gcagcgaatt
     8101 gttgatgaat ctggtcatga atttttgtta cgaggaatgg gacttggtgg ctggatgctt
     8161 caggaaggtt acatgcttca aatgccagga ggtggtaatc agcatcagat aaaagcaaaa
     8221 ataaccgatt tgattggtga cgaagcgtgt gaaagatttt atgaaaaatg gttaagcaat
     8281 catgttacaa aaactgacat tgattcttta gcaaaatggg gattcaatag tgtacgactc
     8341 cccatgcatt ataatttatt taccttacca attgaagaag agccggatcc gtataaaaat
     8401 acttggcttt caaagggttt tgagttgacc gatagcttgg tttcctggtg caaggcaaac
     8461 aaaatgtatg ttattttaga tcttcatgca gcacccggcg gtcagggaaa ggatgcaaat
     8521 atttcggact atgatccaac gaagccttca ttgtgggaga gcgatttgaa taaaaataag
     8581 accatcgctc tttggcagag gattgcggaa cattacaaag atgagcccaa tattggagga
     8641 tatgatttat tgaatgagcc caattggact tttgagaaca aggatattca tggcagagaa
     8701 gatcttttaa ataaagatat ttgggatttg tatatagaaa tttccaaagc cattcgaaaa
     8761 gtagataaaa aacatattct atttattgaa ggtaatggat gggctaataa ttttaatggt
     8821 ttccctggaa aatgggataa caacatggct ttaagtttcc ataaatattg gaaccctaat
     8881 acccagcaat ctattgaacc ttttatacag ttgagaaaga aatatgatat tcctctttgg
     8941 ctgggtgaaa gtggtgaaaa ttccaataaa tggtttacag attgcattcg actggtagag
     9001 aaaaatcata ttggctggtc gtggtggccg cttaaaaaag tttcttctgt ggttaatccc
     9061 tttaccatta ttgctccttc aggttatgat caattgttga actattggaa atatggagaa
     9121 tcaaaacctt caaagaattt tgcaacacaa gttttatttg agttgacgga taatttaaaa
     9181 gctgaaaact gttcttataa caaaggaata atagatgcca tgtttcgcca acgatttgaa
     9241 acaagcactt taccttacac taaaaacttt attccgggaa gaatttatgg agtagattat
     9301 gatttaggtt actacaacag ggcatatttt gataaggata atgaaaatat catggtaaat
     9361 ggcaaaagat ctgttggcaa tcgtggtaat cagtatcgaa atgatggagt agatatcatc
     9421 cgttgtaatg ataaaaatga atatacaaac ggttatcatt tatttgcaat tgaaaaagga
     9481 gagtggctaa aatataccgt agatgtaaaa gagaaaggtg attatcaaat atttgccaga
     9541 attaaaccga cttcttatcc agttaaattt aattttattt ttggaaagaa gaagaaaaaa
     9601 attgaatttt cacatagtga tataaatcca aatgattggc aaactatcta ttgtggaaat
     9661 attaatttaa aaaaaggtaa gcaaaatttg tttatttcct ttgaaagcaa tgattttgaa
     9721 tttaattttt tggaatttat taagaaataa ttcttttcat ttttagtgaa aggtaaatta
     9781 aaaaaatcaa agaaattatt taattagttg aattttagaa aaataaagga gaagtttatt
     9841 atttgatgtt gaggaaaaat gaaaattaaa ttttaggggt gtgagttttt gtgaggtaat
     9901 ttttttggaa cgaattcgaa taagtcatta tttttggatg aattttttta aaattctttt
     9961 tatttattat ttttgttaaa cattattatt aaccaaatta aaaaaaattg ttttatgaaa
    10021 aaattattta cgaaaaaagc tctcatgctt ttatgtgcat tttttatggc tagtgctttg
    10081 tttagccaac agaaacgtta tgtatttgta ttaggtggta atccaacatg gaactcttcg
    10141 tggattaatc ttcgtcttga tgatgttaat agggtcttat atgtatggcc tgatggaact
    10201 tctttgagtg gaacaacacc agtgggtaca ggctcactcg gccaggatgg ttatattgct
    10261 tttacagttg gtaacattgg atggtggggt tgcggttatt tcgttctgcc tacttctcaa
    10321 atggatttaa ctgatgttac cgaagattgg acattccatt ttgccatccg tactgattgt
    10381 gctcaggata ttacagtcgg attaaatgga agctctcctg accctagtga tccttttatt
    10441 actgataaaa cagaaggaaa ggttattctg aacaggacta atttgccgtt agcacaacgt
    10501 gacaaaacac aatgggtaga atttaacatt ccgatgtcac aattaatgag tggaggctcg
    10561 aataatctca aatatttagc acctttaact gatcaaaatt atttgttttt ctcaggtggc
    10621 aacgatgcag gtagctttat tgcatgggat aatgtataca taggagcacc agatggtggt
    10681 tcaggaattt tcactccaaa agctgaaaaa cttgcctttt ctattgacga tgatggtaat
    10741 tatttgaatg tcttcaataa caatagtaat gatgaaatta atatttatag tatttctgga
    10801 gcccatgttt tgaattccaa agttaatcgt gtaaatattt ccaatttatc ctcaggtgtg
    10861 tatattgtga agtcgggtaa tttagtgaat aaatttacaa aaaaatgatt tgaattgatt
    10921 tgtttcattg tacagcttgt ctatgcttca tgtatagaca agctgttttt tcgatttaaa
    10981 aataagcctg cttttaaaag ttcattctct aattaccttt taaaaattca atcctgatat
    11041 tcaacgattt ataaatcaaa aaatatttaa attaattttt ttgaagtagt agattcaaaa
    11101 ataaaacatt tttcaataaa aaagattttc aataaaagta atttcaaata gccaattaaa
    11161 ttattaccta atttaaaaac ttattgggaa tttagaaatt tttatccaat ttgttaagga
    11221 taaatttcat ttgatgataa ttaaaacaat ataataattt ttacacatga aaaaaaagat
    11281 aacgttactt tttctttggg taactttagt catgaatgtt ttttcacaaa cttttaatgt
    11341 tagcggtact gttttatctg ctgaagacaa ccagcctatt attggtgcca atatttggat
    11401 aaaaggagtt aataaaggaa cagtctctga tattaatgga cgttttagta ttgaagtaga
    11461 aaaaaatcaa acactagtca tttcttattt gggctttgaa aatgtagagc aacgtatcac
    11521 tgggccagtt tctaatttga aaattatatt acagccttct actgtagttt tagaagaggt
    11581 tgtagctgtt ggttatggca ccatgaaaaa aagagattta acaggagcta tagcatctgt
    11641 aacgggtgag gatttacgta aactccctgc ggcaactgtt gataaagctt tgcaaggact
    11701 ttcacctggc gttacagcaa ttgctaatag tggtcagcca ggtgctgaca tggttattcg
    11761 tattcgtggc attggtacaa taaatgattc ttcgccaatt tttgtagtgg atggcgttca
    11821 ggtaggtaat attaattttc tttcccctgc tgatataaaa tcagttgaaa tattaaaaga
    11881 tgcttcagcg tgtgctattt atggtgcccg gggtgctaac ggagtaatat taattactac
    11941 tcgacaaggt gataaatcag ggaaaatgaa attgaatgta gaagcatatt atggtattca
    12001 aaataaagcc aaaaaactcg atctaatgaa ttctgaacaa ctttccagat ttttaggcta
    12061 tacaggggat acctctgaag aatttaacca atggatatat gataattttg caggaagtaa
    12121 aacttatatt ccaaaaggga ttgatttttc ttcttatgat accgattggc aagaggtagt
    12181 ttttgatgat aatgcaccca ttcaaaatta ttatgtagct gctgagggag gctctgaaaa
    12241 aactaattat tttttaagtg caggttattt tgatcagaag ggtatcatta tggcttcttt
    12301 ttataaccgt tttacgtttc gtataaattc ttcaacaaaa attaatgact ggctgacctt
    12361 aggggaaaat ctttcattaa tgaattccca taaccggaat gcaccaaata acaatgataa
    12421 ttattctatg ttgaacagtg ctctacggtt tgctccgtgg gaccctgtcc gttttcctaa
    12481 tggcaatgta tccgcttcaa ctattgccaa ttatccaaat cctttaagta tggtagaatt
    12541 tatacatccc aaaaacaatt gggatcgcct tgttggagat gcttatcttc aaattaagcc
    12601 aatcaaggag ttagtatata aagctgattt tggattcgat ctttcttatg ggcagcatag
    12661 ggagttcaaa gataaatatg aagtagctcc ttttgataaa atggataaca attttgttac
    12721 ttccagtttt gaacgttatc agacatggtc tattgaacaa acattgactt atagtaaaat
    12781 attggaaaaa cataattttt cacttatgat tggtcagtca ctttcagaat atagttatta
    12841 ttctttaggt ggttcaagaa ccgacattgc taatccaaga aaagaaaatt ggtatatttc
    12901 tatgggaacg ggtgaatata ctgtaagtga tggggtaggt cgttcgcgtt tggcttcgtt
    12961 tttaggtcga ttgaattatt cttatgccga tcgttatctg ttaacagtta attttagagc
    13021 agatggttct agtaaattcc ccaaagacaa attgtgggga tattttcctt cattttcttt
    13081 aggttggaga gtaagtgaag agaatttttt tatccccttg cgttcagtct ttgattcttt
    13141 caaacttaga gtaggatggg ggcaaattgg aaatgaaaac attccttcta ataattattt
    13201 tgctaaggtt caaacaggtt cgcagtttgt aacttatgtg ttaggtgatg atcagcatat
    13261 ggcttatggc gctacaatga cttctttacc gagcgaatat ctgaaatggg aaacaacaga
    13321 acagacaaat gttggtattg atgtaagttt tttaaatact cgtttaaatg gtagtatcga
    13381 ctattacata agagatacaa aagatatgtt tctgaatgta gttttgccta aacatattgg
    13441 aatgctcttt tcttcacctg ctaatgttgg gaaggttcga aataagggat ttgaattttt
    13501 agttaactgg aaagataaag tatcaaaaga ttttaattat tccataagtg ctaatttatc
    13561 aactgttaac aataaatttg ttgatatgag tatgagttca cctctttatt atgaaggttt
    13621 taaaggtgag aatttagttg tttgcaaaga agggtatcct ctttattcat tttatggcta
    13681 tacttacgat ggtatattcc aaaaccaaca agaggtagat gagtatgtga aaagggatga
    13741 aaatggcgat ccacttcttg atgcaaatgg tgatcttcaa tatattattt caggagtgca
    13801 gccgggtgat gttcgctatg tagataaaaa caatgatgga caaatcacag atttagataa
    13861 atcaattata gggagttcat tccctacttt tacttatgga tttaatatat ttgttggata
    13921 taaaggattc gattgctcat tattttttca aggcgtttca ggaaacaaaa tttataattg
    13981 caataaattt tttatgcaag gaggcgaagg gataaaactt actaatttag gtacagaaat
    14041 gctccattct tttactactg atcagccttc cactacgata ccgcgtgcta aaggacgtgc
    14101 ttctaattat tgggcttctg ataggtttct tgaagatggg agttatttgc gtttgaaaaa
    14161 tgcacaaatt ggttacagtt tacctccaaa agtattatcg aatgctaaaa ttgagtcgtg
    14221 tcgcctttat atttctggag caaatttatt aacttttacg aactatactg gttatgatcc
    14281 agaagcgagc tttcgtggag tggatagagg taattatcct caagctgtca cttttttgtt
    14341 tggtgttcag ttgggtttat gagaaattga ttttattatt ttaaatggat aaaaaatgaa
    14401 aaagattata tatttatttt ttttcgtagt aagtggatgt cttatgggat gtactgattt
    14461 tttaacacct gagccactag caacagaaaa attaagtgat ttttttaata atgattcagc
    14521 agcaatacaa gcagttaatg cagcttatgt gcctttacaa tggcagttgg ggggtaccta
    14581 cgctaatgaa tggtttattg gtgatgtggt ttctgatgat gctttaaaag gtggagcttc
    14641 tttatatgat atgcaagtgg tgggtttaat ggaaaatttt caaaccacag ctaataatga
    14701 atatttagat actttttatg acatcaatta tcagggaatt tttcgttgca attttgctat
    14761 ggaaaatatt aatggaatga ctgctgataa atggtctgac caacatctaa agacaaggtt
    14821 gattggcgaa gcaaaatttt taagagcctt gtatcatttc cgtttgatga gagtttttgg
    14881 cggtattata aagggagaca ggacttttaa tcaacatgat acacaaatag cacgttcaag
    14941 taaagcggaa attgtaaggt ttattcgtca agatctggag gatgcgatta aagctcttcc
    15001 tttaaaaagt gaatatcctg cttcagaatt gggaagagca acaaaaggag ctgcccaggc
    15061 tttattgatg agggttattc ttttcgacat tcttaatagt gaaaatccct ttggtgatta
    15121 tgatagagta atgtctttag gtgatagtat tatagcatct aaagagtatc gtcttgaaga
    15181 taattatgct gatattttta aagtatttga aagtcccaca gaagcaagat ttgaaaaaga
    15241 aaatgggcca gaatccgttt ttgaaattca atacatggaa gaagccactt cagattacgg
    15301 aggattaggc agaacaagag gaaattttga cccaattatg actcgcactc gtgctaataa
    15361 ttctgggtgg ggattcaatc gtcctacgca agatttgtat gatgaatttg aacctgatga
    15421 catacgtcgt gatgaagcta ttttgccttt tgatgcggga gatgtttatg ctcctggtag
    15481 ttatgataat ggttatcatg ccaggaaagc tgctttggga ttttggagaa cagtaggtgg
    15541 caaacaagtt ttttatatgc ctcccttaaa acatccaaca cgcgcaccaa ataatactat
    15601 agttatcaga tatgcagatg taattttaat gtatgcagaa gcagcatgtg aaataaataa
    15661 catcaaagaa ggaaaagaca aattaaactt gattcggttg cgtgctcgaa attcagcatc
    15721 cgatcccaca aatcctgata ttttgccaga tttcccttat ggatcctatg ttgaaaatca
    15781 agttgatttg cgcaaagcta ttcgtcatga gcggcgtgta gaatttgcta tggaaggtca
    15841 tcgttggttt gatttaataa gatgggggat tgctggcgat gtgatgaatg catatcgaga
    15901 gaaatatatt gcaaaagagg gtaaagatat gagtcctttt ataaaaggta aacatgaatt
    15961 gttccctatt cccgaaacac aattaaatgc taatcctctt ttacaacaaa atccaggtta
    16021 ttaatatatt taatgtaatt tatcatgagt aaaaaatcaa ggaagttatt caaaattatt
    16081 cttattttat tgttattcat tataattcct gtaaagagtc aaaactggca attggtttgg
    16141 tcggatgaat tcaatggaac aactttgaac acggataact ggaatatagt ggaaggggga
    16201 agtggttttg gtaatcagga aaaacagttt tatacttcgc gtcctcaaaa tttaaaggtt
    16261 gaaaacggta atttggtaat aacagccttg ttagaaaatt ataccgttgg tagtatgacc
    16321 tggaactaca cttcagcaag aatcaatact tcacgaaaga aagattttac ttatggaaaa
    16381 attgaggccc gaattaaact tccaacaggt cgtggtacat ggccagcttt ctggatgatg
    16441 ggatatggaa gttggccttc atgtggtgaa atagatattc tggaatttgt tgggcgccaa
    16501 ccagacagat ttcaatcaaa tgttcatacc aaagattata atggtatgaa tggacaaaat
    16561 ttccatatag aaaaagtgta tcctggtctt ccaaacgatt ttcatgtata ttcgatagaa
    16621 tggacttctg aaaaaatcaa gtttttcttc gatggggcac aatattggac tttttcttcc
    16681 ctttcagtag atcctgcaaa ctacccattc aacaatcctt tttatataat tttaaatttg
    16741 gcaattggag gaacgatggg tggtgctgtg gataatagta ttttcccaca aaaattatta
    16801 gtggattatg tgagagttta tagggatatc aatgcagtta atgatccatc tagtaagcaa
    16861 cagccggttg tatcaacgct ggtttctgat aatttaaata ttcaaattcc tgaaaatcaa
    16921 agaaataaca aaataataat ctatgatttg aatgggaaaa cgatcaggca acaaaacact
    16981 tctgagcgtg agttaaatat aaatgtatca gatttaacta aaggtgttta tattgtaaaa
    17041 atagtatccg atgaaaatat ttatacaagc aaaataatta aacaatgatc agaaatatct
    17101 tacattttaa tattaagaga aaagtttttt ccttcacaat tattttgatt tttttttgta
    17161 taagtgattt gtgtgctcag aaaagaagca ccaaaagagg attagcatat agtattcctt
    17221 atgttgaaga tttagttcct ttgagtagag ggatttcctg gttttataat tggggaattt
    17281 ttccttccag tgctatagat aattattata gtaattatat ggaatattat cctatggcat
    17341 ggaatggagt aaatgctgat ggattaaggg catttaaagc aaaacatcct gaatgtcagt
    17401 acttgttggc ttttaatg
//
LOCUS       DAOSAS010000023        17028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_2322_length_17028_cov_11.437518, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000023 DAOSAS010000000
VERSION     DAOSAS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17028
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2322_length_17028_cov_11.437518"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            585..1838
                     /locus_tag="PLR72_01975"
     CDS             585..1838
                     /locus_tag="PLR72_01975"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK36012.1"
                     /translation="MGIKRVIKQQAKEIFRQLKSNETLIILCIEVTIMMMGIGLVSPI
                     LPQYARTFGVNMTLIGMLITIFGIARMIVDIPAGRLTDKIGRRPVLIIGPLILAIGSI
                     GCGLAQNYWLFLSFRFIQGIGSAMYTTAAMVMLADLSTENNRGKMMSFYQGCILLGAG
                     LGPTMGGFLAQYFGLRIPFFAFAALAVSASIWAYFSLPESRPEKIYKQSKNQEVLLDS
                     HQKAIKNLLRDPNFLLISLITFGVFITRQGAQNQILPLLSSDRLGLNEGQIGFALTLV
                     AVVQLVTIFISGRLSDRIGRKAVITPGCIIIAISLIMLSYTNSYSFLIFTCLIMGIGI
                     GTCGPISSAYVADIIPKESYSVGMGTYRAIGDLGFVIGPVLLGWLCDVKNFDFALLCN
                     SLFLFGVVLLFQFKAEESHCVSWKV"
     gene            1967..2620
                     /locus_tag="PLR72_01980"
     CDS             1967..2620
                     /locus_tag="PLR72_01980"
                     /inference="COORDINATES: protein motif:HMM:NF022902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36013.1"
                     /translation="MIQKLKVNKTELGSKTAKGGFANEKAVCEKFEAWDRDIEAQEWL
                     KIMGYDLKKISSVKAIQIPVKIKKSELSEFDITDEIEYENFIKFKKADAQIKITIKMG
                     EIVKIENISLKKANSNADYNQIDKRSVDNYQEMWNFDDEIALWLKFFTGELNPNKYLN
                     LMPVKKFKDARRLFFNEMPERIQNKIIEFFEQNRIVIVSDILKGRGCLSTQWMLDKV"
     gene            2643..2822
                     /locus_tag="PLR72_01985"
                     /pseudo
     CDS             2643..2822
                     /locus_tag="PLR72_01985"
                     /inference="COORDINATES: protein motif:HMM:NF022902.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2831..3622
                     /locus_tag="PLR72_01990"
     CDS             2831..3622
                     /locus_tag="PLR72_01990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOK36014.1"
                     /translation="MNKVYLGDCIEIMKNLPEKTVDLIFADPPFNIGIKYDIHNDNMS
                     YDDYYHWSESWIKECFRLLKDNGSIYIAIGDEFAAEINIILKKTGFNFRNWIIWYYTF
                     GQNQRKKFNRAHTHILYFTKSKEHFIFNDSQIRIPSARQLVYKDKRANPIGKIPDDVW
                     QFSRVCGTFKERIGKHPCQMPEDLLELIIKTSSNKGDIVLDPFGGTGTTVAVAKKLKR
                     NYLTMEMSQDYYTILLYHHFKTAGRENSFFRKEEHRKTGETKNII"
     gene            complement(3860..4687)
                     /locus_tag="PLR72_01995"
     CDS             complement(3860..4687)
                     /locus_tag="PLR72_01995"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOK36015.1"
                     /translation="MFHQRNKFFRFFFLISIILFLIAACNRRPKDVLSKKEMVKVLTE
                     FHLLDGVLYAKDSLYIGNYNTYYYQYVLKKHGVTQAQFDSSLAWYGKNPREFEKVYTD
                     VILRLNHLQEDIKKEKYHPNYLETTKTNLWYLPTHYLLTKDSIRRNLLFKIQRSDLLF
                     NDIYELKLLQRIAPEDSCENRYMVLRIHYANGEVDSISHIAYNDSLLRRYTFRLRAKQ
                     PYKIDSLTGSLLNSIDCNEKLNVEIDSVSLIREFVPLWQDSLRKIVNKNDSLFPSEN"
     gene            complement(4668..5279)
                     /locus_tag="PLR72_02000"
     CDS             complement(4668..5279)
                     /locus_tag="PLR72_02000"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOK36016.1"
                     /translation="MKMSIAKKSALLIFLVLFIDQASKIYVKTHFALGDHVDIFNWFQ
                     IYFIENSGMAFGMQFIGKLFLTFFRIAAVVLIFFYLRSLIKKQFSTGYILTISLLLAG
                     AAGNIFDSVFYGVLFSDSYGKVATFLPPEGGYAPLFYGKVVDMLYFPLIKNSAGEVIF
                     FKPIFNVADSAITIAVFIILFFYRKELNQSFEKKPKQNVSPEK"
     gene            complement(5341..5718)
                     /locus_tag="PLR72_02005"
     CDS             complement(5341..5718)
                     /locus_tag="PLR72_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOK36017.1"
                     /translation="MAEKTRYSDEELEEFRQIILEKLEKAQKDYEILRNAITNEASND
                     VSDTSPTFKVLEEGATTLSKEEAGRLAQRQLEFIRHLQAALVRIENKTYGICRETGKL
                     IPKERLRAVPHATLSIEAKNKKK"
     gene            complement(5822..9310)
                     /gene="ileS"
                     /locus_tag="PLR72_02010"
     CDS             complement(5822..9310)
                     /gene="ileS"
                     /locus_tag="PLR72_02010"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOK36018.1"
                     /translation="MKKFTEYSELNLSEINKEILTWWNEQNTFLQSIAIREGKPSFVF
                     YEGPPSANGMPGIHHVMSRTIKDIFCRYKTMQGFQVKRKAGWDTHGLPVELNVEKLLG
                     ITKEDIGKKISIEEYNNVCRREVMRYTQEWEDLTMKMGYWIDMKHPYITYDNRYIETV
                     WWLLKQLYEKKLLYKGYTIQPYSPAAGTGLSTHELNQPGCYRDIKDTSCVAQFKIKEI
                     EKLPFKDKIEGDCYFLAWTTTPWTLPSNTALCVHPNISYALVKTINPYTGKLIYVILA
                     KDLIDKYFSPKNADLSFENYQPGDKNIPYEMLAECSGKELVGIHYEQLIPWVKVEGDA
                     FRVISGDFVTTEEGTGIVHIAPTFGADDDRVAKQNNIPPLLLKDKEGNWQPMVDKTGK
                     FFKLEDLDRDFIAQKINVELYQPYAGRYVKNEYLPEEIQLEDSITTENGINDEATLDI
                     DLCVMLKQQGKIFKIEKYVHSYPHCWRTDKPVLYYPLDSWFVKTTAYRDKMIRLNETI
                     NWKPSSTGTGRFGKWLENLQDWNLSRSRFWGTPLPIWRTEDGSEEICIGSVEELMVEI
                     EKSVAAGLMKKNPYKNFKVGVYTDENYSIENIDLHRPYVDEIILVSESGKPMRRESDL
                     IDVWFDSGSMPFAQLHYPFENKEAIDKHKFYPADFISEGVDQTRGWFFTLHAISTMIK
                     NSVAFKNVVSNGLVLDKNGNKMSKRLGNVIDPFATIEKYGSDPLRWYMMTNSSPWDNL
                     KFDIDGVEEVRRKFFGTLYNTYSFFALYANVDHFTYAEEEIPVEKRPEIDRWIISLLN
                     TLIKEVTNDYDDYDPTRAGRAIADFVCENLSNWYVRLNRKRYWGGEYDEDKISAYQTL
                     YTCLEIVSQLMAPIAPFYADQLFIDLNAVTEKHKEASVHLTDFPKYNENLIDKNLEEC
                     MQLAQQVSSMILSLRKKANKRVRQPLAKAIVPAPDEKTYQQLKYVADLIKTEVNVKEL
                     EIIPQNGKMHNLIKKVKPNFKLLGKKYGKLMKEITEALANLGNTEIAEIEQKGEYVLQ
                     LSAGEVPISLEEVEIITEDMPGWLTANDGKLTVALDITVTDELLKEGIAREFVNRIQN
                     IRKANDFEITDRITIEIEKRDEINEAINEFSNYIASQTLATSITLVDKLDNTTELDFD
                     DYFVKIKIKKTKEFIQ"
     gene            9847..10974
                     /locus_tag="PLR72_02015"
     CDS             9847..10974
                     /locus_tag="PLR72_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOK36019.1"
                     /translation="MKHYKFLLFAFVLLLALISYVSCRGWQVLTLIHSTVGRKSYLIV
                     CILLTICLFFGMFFEDKLSISLARPLLFLGYSFLLLVTYLFFSFLLVDIFRGLNHFLH
                     FISDQGMIALRFWTMMASLVLIMVLMIAGNIKFNHPALVNLYLKSNKSVQNKEIKIVA
                     VSDLHLGTCIDKKQLQKYITLINAQHPDLVLMVGDICDRSLPAMVHQNLKEDLQKIQA
                     PLGVYAVDGNHEYYSFHLAEVNEYYKNAGVNVLRDSTVEIQHQFYLVGRDDYTNRQRK
                     KLSELMDSFDPSKPVIVVDHQPMNLEESQEENIDLHLSGHTHNGQVFPGNLLAKRFFE
                     VSYGYKKKRNTHIYVSSGLGLWGPSYRIGTQSELVAIHFQY"
     gene            complement(11104..11517)
                     /locus_tag="PLR72_02020"
     CDS             complement(11104..11517)
                     /locus_tag="PLR72_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36020.1"
                     /translation="MKKTLIVMLCTLFTLSVTIFAQKENQPQQMKKEMRQNPPKRMSA
                     EERANYWAKELNLTDEEKAKVTELFKKEDENRAKYREEMQRNREEMRAQMQKDRKAND
                     AELEKIIGKEKFQKYDSIRSEQMKNRKRTPTTKGK"
     gene            complement(11693..13822)
                     /gene="scpA"
                     /locus_tag="PLR72_02025"
     CDS             complement(11693..13822)
                     /gene="scpA"
                     /locus_tag="PLR72_02025"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOK36021.1"
                     /translation="MKPDFSKIDVQNIIPTSNEEAVEVSTWMTAEQIPVKSFYTKEDE
                     KGIQHLNYAAGIPPFLRGPYSTMYVTRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGL
                     SVAFDLPTHRGYDADHERVVGDVGKAGVSICSVEDMKILFDGIPLNKMSVSMTMNGAV
                     LPILAFYIVAAQEQGAKLEELSGTIQNDILKEFMVRNTYIYPPQFSMRIIADIFKYTS
                     KNMPKFNSISISGYHMQEAGATADIELAYTLADGLEYLRTGLNAGLDIDAFAPRLSFF
                     WGIGMNHFMEIAKMRAARLLWAKIVKQFNPKNPKSMALRTHSQTSGWSLTAQDPFNNV
                     GRTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYLQEETYICRQIDPW
                     AGSYYVESLTKEISEKAWSLIQEIEELGGMAAAIETGIPKMKIEEAAARTQARIDSGE
                     QVIVGVNRYCLEKEEPIEILEIDNTAVREQQIERLKQLRATRNNEEVQKALDAITECA
                     RTGNGNLLELAVEAARVRATLGEISYACEKVAGRYKATIRTISGVYSSETKENADFKR
                     ACELTEKFAKKEGRRPRIMIAKMGQDGHDRGAKVVASGYADCGFDVDMGPLFQTPEEV
                     AKQAVENDVHVLGISSLAGGHKTLIPQVIEELKKLGREDIMVIAGGVIPPQDYDYLYK
                     AGVAAIFGPGTSVAKAACTIMEMLLEE"
     gene            complement(13966..14580)
                     /locus_tag="PLR72_02030"
     CDS             complement(13966..14580)
                     /locus_tag="PLR72_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOK36022.1"
                     /translation="MKKVVLPILIALVLWFFMFSPWTRSYINFWIVMSCAAVFFIFIS
                     AFFGKNFKKQFSFSIKDIFIGIGSAIVLYGIFQLGYVFSKLIFVFAEEQVGNIYLIKS
                     GENTLYLGLLLAFLIGPAEEIFWRGYVQRMLEPQYGEWSAFAITAITYTFIHLWSFNF
                     MLILSALVCGIFWGLLYKYNKNLVTLIVSHALWDVSVFILFPIS"
     gene            complement(14577..15479)
                     /gene="menA"
                     /locus_tag="PLR72_02035"
     CDS             complement(14577..15479)
                     /gene="menA"
                     /locus_tag="PLR72_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOK36023.1"
                     /translation="MKSKLNNYFIATRPWSFPASTMPALITLTYIFYINEEISGNINW
                     WYGIIAIIGAAIFQASSNLINDYFDYKYQVDRPESFGSSRLLVEKVFTPKFILSYGFV
                     LLGIGVLLGLFLWYKTGNALLWIGIIGTLAAVLYSKLKYVALGDLIIFIIYGPLIGLG
                     TAYVMTQQLLWKIILINIPIAFLVVNILHANNTRDIRDDNKAHIKTQSMVLGLKRAKI
                     QYVTLAVGAYVMIALLIILRMIHPIVLLVFLTVPLTIKNIRQMQTAQVEKPELIKDLD
                     AKSAQLVLLFGLIFSICNMIGNWL"
     gene            complement(15512..>17028)
                     /locus_tag="PLR72_02040"
     CDS             complement(15512..>17028)
                     /locus_tag="PLR72_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOK36024.1"
                     /translation="LLENINAECIELNFLVDANNAITLANTLSEYLLSQQYDVKNLQG
                     AIEFDPIGEMLQDGKKKSKEQIVEQLKSVIDAISKLPFYRVMTVNATILKNAGAFITQ
                     ELGNALAWGNQYLSMMVDAGVDTSLAAKKIKFNFGIGSNYFMEIAKFRAARLLWALIV
                     DAYQPVCSCEDNSNAKDGTCKCATKMRIHAETTNFNKTIFDAHVNMLRTQTEAMSAVL
                     GGIDSLTVLGYDDVFNAPNEFAERIARNQQLLLKEESHFDKVVDPAAGSYYLEILTKE
                     IAQQAWKLFLEIENEGGFLEAIYAGKVQQAIKATANYRMKNISNRKEILVGTNQYPNF
                     NETASEKLVNCPQCDKNNQSTEVETLPPVRGAAEFETLRLATERAAKRPKVFMLTIGN
                     LAMRVARSQFSCNFFACAGYEVIDNVGFDTVEEGVQAALEAKADIVVLCSSDEEYAQF
                     APAAYQLVKDKAIFVVAGAPACMDDLKTQGIENFISVKSNVLEILKSFNEKLGI"
BASE COUNT         5498 a         2956 c         2810 g         5764 t
ORIGIN      
        1 aagagaccaa agtaaagcat ttattccatc agggcaaagg ggaaatgctc tttttctcct
       61 ttgtcttgaa aaataatttt tctttacctt ttatttcccc tgaaaaagtt gcattaatta
      121 cttgaattta cgtttttata taatcagttt attctaatcg ttgaaaaaac caacttacaa
      181 aggtaacatt tgtgattaga gccaccgtat ggtaagccgt taaagttatg tgaccttgat
      241 tttttatttt cgtgcacttt ggaaataccg cgattttcat gtacctttgc aaaaatttta
      301 aagtctttcc tttagaaagt agccatctga ttatcagcaa aaatggttct tccaattaat
      361 aagtcaactt ttctcgagta aaaaattctt attaattgct tcttccagca aaacaaattg
      421 caatattttt tcaggaaaaa attactcatc gttgagaaag gagagagggg aaaatcattc
      481 aagtaaataa ttgaaaggaa ttaactctta taatgaaaaa actttataaa aaaataggaa
      541 agtcattatg gtaagaaaat ggaaaattta gaaaggaata aaatatgggt ataaaaagag
      601 taataaaaca acaggctaaa gaaatatttc gccaattaaa aagtaatgaa acattaatta
      661 tcctttgtat agaagtaacc attatgatga tgggcattgg tttagtaagc cccattcttc
      721 ctcaatacgc tcgtactttt ggtgtaaata tgacactcat tggcatgctg attactattt
      781 ttgggatagc tcgtatgata gtggatattc ctgctggaag attgactgat aaaattggac
      841 gacgtccagt tttgattatt ggtcccttga ttcttgccat tggttctatt ggttgcggtt
      901 tggctcaaaa ttactggtta tttttatctt tcagatttat tcagggcatt ggctctgcca
      961 tgtacacgac agcagcaatg gtgatgttag ccgatctcag cactgaaaac aatcggggta
     1021 aaatgatgag tttctatcaa ggttgcatac ttcttggagc aggtttagga cccacaatgg
     1081 gtggttttct tgctcaatat tttggattaa gaattccttt ttttgctttt gctgcactgg
     1141 ctgtaagtgc aagtatttgg gcatatttta gtttgcctga atctcgccca gaaaaaattt
     1201 acaagcaatc aaaaaatcaa gaagttttat tggattctca tcagaaggct atcaagaatt
     1261 tgttgcgaga ccctaatttt cttctcattt cactgatcac ttttggtgta tttataactc
     1321 gacaaggagc tcaaaatcag attttaccat tgttgagttc agatcgtttg gggttgaatg
     1381 aaggacaaat tggttttgct cttacgttag tggcagtggt acaacttgtt actattttta
     1441 tcagtggaag gctttctgat cgtattggca gaaaagctgt tattacgccg ggatgcatta
     1501 tcattgctat ttcattgatt atgctctcat atactaacag ttattctttt ttaattttta
     1561 cttgtcttat tatgggaatt ggaattggaa cgtgtggacc tatttcttca gcttacgtgg
     1621 cagatattat tcccaaagaa agttacagtg ttggaatggg tacatatcgt gctataggtg
     1681 atttgggctt tgtaataggt cccgttttat taggctggct ttgtgatgtc aaaaactttg
     1741 attttgcgtt actatgcaat agcctgttct tgtttggtgt tgttttactt tttcaattca
     1801 aagccgaaga aagtcattgt gtctcatgga aagtgtaggt tttaaagtga aaaaaattga
     1861 ggaatgattt tttaatagaa ttaaaaaatt aactgttact ttgaatcata ataaaaaaaa
     1921 cataattttg tgaacatgta taattttcta tgagatcttt attactgtga tacaaaaatt
     1981 aaaggtaaat aaaactgaac tcggttcgaa aactgccaaa ggtggatttg caaacgaaaa
     2041 ggcagtttgt gaaaaatttg aagcttggga tagggatatt gaagcgcaag aatggcttaa
     2101 aattatggga tatgatttaa aaaagatcag ttctgtaaaa gccatacaaa tacctgttaa
     2161 gattaagaaa agtgagttaa gtgaatttga tattactgat gaaattgaat atgagaattt
     2221 tataaaattt aaaaaagcag atgctcaaat aaaaatcacc atcaaaatgg gtgaaatagt
     2281 aaaaattgaa aatatctcat taaaaaaagc caattctaat gcagattata accaaattga
     2341 caaaagatct gttgataatt atcaagaaat gtggaatttt gatgatgaaa ttgctttatg
     2401 gctaaagttt tttactggtg aacttaatcc aaataaatat ttaaatttaa tgccagttaa
     2461 aaaatttaaa gatgccagac gcttattttt caatgaaatg ccagaaagaa ttcaaaataa
     2521 aattattgag ttttttgaac aaaacagaat tgttatcgta agcgatattt tgaaaggaag
     2581 aggctgttta tcaactcagt ggatgttgga caaggtataa tggtgagaag gaaacaactg
     2641 actggatatt gaaagatatc aatgaagcta tgaatttctt tggaaaagga gaagttacca
     2701 tttcaccaaa gggaagcctt tatattggga aaattactct tcaaagaaaa ggtggaactc
     2761 ccgatcctac aaaactacaa tttaaaatta aaccttgtca attatttagt ttaggagaat
     2821 agatatggag atgaataaag tatatttagg agactgtata gaaataatga aaaatttacc
     2881 agaaaaaaca gtagatttaa tttttgcaga tcctcctttt aacattggta taaaatatga
     2941 tattcacaac gataacatgt catacgatga ttattatcat tggtctgaaa gttggataaa
     3001 agagtgtttt cgattgctta aagataatgg ttctatttat atagcaatag gtgatgaatt
     3061 cgctgctgaa atcaatatta ttttaaaaaa aacaggattt aatttccgca attggattat
     3121 ttggtattat acttttggac aaaatcaaag aaaaaaattt aatcgggctc atacccatat
     3181 tttatatttt acaaaaagta aagaacattt tatttttaat gattctcaaa ttagaattcc
     3241 ttccgcacgt caacttgttt ataaagataa aagagcaaat cctataggta aaattcctga
     3301 tgacgtttgg caattttcaa gagtatgtgg aacttttaaa gaaaggatcg ggaaacatcc
     3361 ttgtcaaatg cctgaagatt tactcgaatt aatcataaaa accagttcca ataaaggtga
     3421 cattgtactg gatccatttg gtggaacggg aacgacggta gctgttgcta aaaaattaaa
     3481 acgtaattat ttaacaatgg aaatgtcaca agattattat accatattat tataccatca
     3541 ttttaaaacg gctggaaggg aaaattcctt ttttcggaag gaagaacaca gaaaaacagg
     3601 agaaacaaaa aacattattt gatattgaat aaaaaaataa attttttact ctttgaaaaa
     3661 ttgtttgctt taagaaatag gaaaaataaa atgaaaatta tgctgactga aattctgtta
     3721 cttgcaaatt attcaaagat cgaaaataaa attcagagaa gatattttta tttttcgcta
     3781 taatttttct tctaagtaca agattacaaa taaattaaaa acgttcttaa agtttttttc
     3841 tttatgaaga tcttttttat taattttcgc ttgggaaaag ggaatcattc ttgttgacaa
     3901 ttttccgtaa actatcttgc cagagaggga caaattcccg tatcaaactc acgctatcaa
     3961 tttcaacatt cagtttttcg ttacaatcta tactattcaa caacgaacca gtaagtgagt
     4021 caattttata aggctgcttt gcacgtaaac ggaaggtata acgacgcaac aaactatcat
     4081 tatatgctat gtgagaaata ctgtcaactt ccccattggc ataatgaatt cttaaaacca
     4141 tataacgatt ttcgcaagaa tcctcaggag caattcgttg caatagtttc aattcgtaaa
     4201 tatcattgaa aagaagatca gatcgctgga ttttaaatag caaatttctt ctgatagaat
     4261 ctttcgtaag aagataatgt gtaggcaaat accagagatt tgttttagta gtttcaagat
     4321 aattgggatg atatttttct tttttaatat cttcttgtaa atgatttagc cgaagaatca
     4381 cgtcagtata aactttttca aattctctgg gattttttcc ataccatgca agagacgaat
     4441 caaattgagc ctgagtaaca ccatgttttt ttaaaacata ttgataataa taggtattat
     4501 aattaccaat gtaaaggctg tcttttgcat aaagtactcc atcgaggagg tgaaactcag
     4561 taagaacttt aaccatttcc tttttactca gtacatcttt aggacgtcga ttgcacgcgg
     4621 ctattagaaa tagaataata cttattaaaa agaaaaatcg aaagaattta tttctctggt
     4681 gaaacatttt gttttggttt tttctcaaaa ctttgattca attcctttcg gtagaaaaac
     4741 agaatgatga aaacagcgat cgttatagca gaatctgcca cattaaaaat tggtttgaaa
     4801 aaaattactt ccccagcact atttttaatc agaggaaaat aaagcatgtc taccacttta
     4861 ccataaaaca agggagcata accaccttca ggaggcaaaa aagtagccac tttaccataa
     4921 ctgtcactga aaagaacacc ataaaataca gagtcaaaga tatttcctgc agcacctgcc
     4981 agcaaaagcg aaatagttaa aatatatcca gtggaaaatt gtttttttat taaggaacga
     5041 agataaaaaa atattaacac aactgctgca atacgaaaaa aggtaagaaa aagtttaccg
     5101 atgaattgca ttccgaaagc cattccgctg ttttcaataa aataaatttg aaaccagttg
     5161 aatatatcca catggtcacc cagagcaaaa tgagttttta cataaatttt tgaagcctga
     5221 tcaataaaaa gaacaagaaa aatcaacagg gcagattttt tagcaataga cattttcatc
     5281 tggaggtcat tatgaaagtt cggcagcaag ttttttgctg ccgacttttt gcttgaagat
     5341 ttattttttc ttattttttg cttcaatact taaagtagca tgaggaacgg cacgcaatcg
     5401 ctctttaggt atcaattttc ctgtttcacg acaaattccg taagttttat tttcaatacg
     5461 taccaatgct gcctgtaaat gccgaatgaa ttctaattgc cgttgagcca atcttcctgc
     5521 ttcttctttt gaaagggtag tagctccctc ttctaacact ttaaatgttg gcgaagtatc
     5581 tgaaacatca ttactggctt cattagttat cgcattacga agtatttcgt aatctttctg
     5641 agccttttca agcttttcaa gaataatttg gcgaaattct tccaactcct catctgaata
     5701 tcttgttttt tctgccatcg ttcattgaat gataaaagta atcctttcgg attggttaat
     5761 gcctttgaaa taatctgcga aaataatcat tttttttctc tttagcaata attttaaaaa
     5821 ctcattgaat gaattccttt gtttttttta ttttaatctt tacaaagtaa tcatcaaaat
     5881 ctaattcagt ggtattatca agtttatcca ctaacgtaat agaggttgcc aatgtctggc
     5941 tggcaatata atttgaaaat tcgtttattg cttcattaat ttcatcccgc ttttctattt
     6001 caatggtaat tctatctgta atttcaaaat cattagcttt tcgaatattt tgaattctat
     6061 tgacaaattc acgagcaata ccttctttca gcagttcatc cgtcaccgtt atgtcgagtg
     6121 ctactgtcag tttgccatcg ttagcagtta accagcctgg catatcttct gtaataattt
     6181 ctacttcttc cagagaaata ggaacttctc ccgctgaaag ttgtaaaaca tattcgcctt
     6241 tttgttctat ttctgcaatt tctgtatttc ctaaattagc cagagcttcc gtaatttctt
     6301 tcattaattt gccatatttt ttaccaagca atttgaaatt tggctttact ttctttatta
     6361 aattatgcat tttaccattt tgcgggatga tttccagttc cttcacatta acttctgttt
     6421 taatcaagtc cgctacatat ttcaattgct gataagtttt ttcatcgggg gctggaacaa
     6481 tagcttttgc taatggttga cgcactcttt tatttgcttt ttttcgtaac gaaagaatca
     6541 ttgaagaaac ttgttgagcc agttgcatac attcctccag atttttgtca ataagatttt
     6601 cattgtattt ggggaaatca gtcaaatgaa cagatgcttc tttatgtttt tccgtaactg
     6661 cattcaaatc gatgaacaat tggtcagcat aaaaaggtgc aatgggtgcc attagttgag
     6721 aaacaatttc taagcaggta taaagtgtct gataggcaga aattttatcc tcatcatatt
     6781 caccacccca ataacgtttc ctgttcaaac gaacatacca attgcttaga ttttcgcaca
     6841 caaaatctgc aatagcgcgt ccagctcgcg taggatcata atcgtcataa tcattggtaa
     6901 cttctttgat caaagtatta agaagggaaa ttatccatcg atcaatttca ggacgttttt
     6961 ccacaggaat ttcttcttcg gcataagtaa aatgatccac attggcgtaa agagcaaaaa
     7021 aggaataagt attgtaaagc gttccaaaga atttccgacg aacttcttct actccatcaa
     7081 tatcaaattt taaattatcc cacggtgaag aatttgtcat catgtaccag cgaagaggat
     7141 cagaaccata tttttcaatc gtcgcaaaag gatcaataac atttcctaaa cgttttgaca
     7201 ttttatttcc atttttgtca agaaccaaac cattggaaac cacgttttta aaggcgacag
     7261 aattttttat catggtactg atggcgtgca aggtaaaaaa ccatccacgt gtttgatcca
     7321 ctccttcaga gataaaatcg gctggataaa atttatgttt atcaatggct tctttatttt
     7381 caaaaggata atgcaattgg gcaaaaggca tcgaaccaga atcgaaccag acatcaatta
     7441 aatctgattc tctccgcatc ggtttgccac tttctgacac aagaataatc tcatcgacgt
     7501 aaggacgatg caaatcaata ttttctattg aataattctc atcagtataa acgccgactt
     7561 tgaaattttt ataaggattt tttttcatca atcccgcagc aacagatttt tcaatctcta
     7621 ccatcaattc ttctacggag ccaatgcaaa tttcttctga tccatcttct gttcgccaaa
     7681 tgggaagagg cgttccccaa aatctacttc gtgacaaatt ccagtcctga agattttcca
     7741 gccattttcc aaatcgtcct gttcctgttg aggaaggttt ccaattgata gtttcgttaa
     7801 gacgaatcat tttatcccga taagcagtag tttttacgaa ccaactatcc agcggataat
     7861 acaaaacagg tttgtcggta cgccagcaat gtggataact atgaacatat ttttcaattt
     7921 tgaaaatttt gccttgctgt tttaacatca cacaaagatc aatatcgagg gttgcttcat
     7981 catttattcc attttctgtt gtgatggaat cttccaattg tatttcctct ggaaggtatt
     8041 cattttttac atatctacca gcataaggtt gataaagttc tacattgatt ttctgagcta
     8101 taaaatcacg atccaaatct tccaatttga aaaatttacc tgttttatcc accattggtt
     8161 gccaatttcc ttctttatct ttcaacaata aaggaggaat attattttgt ttagctactc
     8221 tgtcatcatc tgctccaaaa gtaggggcaa tatgaacgat gcctgttcct tcttcggttg
     8281 taacaaaatc acccgaaatt actcgaaaag catcaccttc cacttttacc cacggaataa
     8341 gttgttcata atgaatgcct accaattctt ttccagaaca ttctgccagc atttcataag
     8401 gaatattttt atcaccgggt tgataatttt caaacgataa atcagcattt ttaggtgaaa
     8461 aatacttatc aatcaagtcc ttagctaaaa tcacataaat gagcttacca gtataaggat
     8521 taatagtttt aaccaaagca tacgaaatgt ttggatgaac gcacaaagct gtattagaag
     8581 gcaaagtcca gggtgtagtg gtccatgcca agaaataaca atctccttca attttatctt
     8641 tgaaaggtaa cttttcaatt tctttgattt taaactgagc tacacaactg gtatctttta
     8701 tgtcgcgata acaacctggt tgatttaatt catgcgtact caatcctgtg cctgcagcag
     8761 gagaatatgg ttgaatagta tatcctttat agagcaattt tttttcataa agttgcttca
     8821 acagccacca tactgtttca atgtaacgat tgtcgtaggt aatgtaagga tgtttcatgt
     8881 cgatccaata acccattttc atggtaagat cttcccattc ttgcgtatat ctcattactt
     8941 cgcggcgaca gacattattg tattcttcaa tgctgatttt tttcccaata tcttctttcg
     9001 taattcctaa tagtttttcc acattcaatt ccacaggaag tccgtgagta tcccaacccg
     9061 cttttctttt tacttgaaat ccttgcattg ttttatatct gcagaaaata tctttaatag
     9121 tacgtgacat tacatgatga atgcccggca taccattggc tgaaggtgga ccttcataaa
     9181 agacaaaaga aggttttcct tcgcgtatag caatgctttg aagaaatgta ttttgttcgt
     9241 tccaccatgt aagtatttct ttattgattt cagaaaggtt taactctgaa tattctgtaa
     9301 attttttcat gttcgaaagc acttgatata ctattttttt gaagttgcaa aattacgaaa
     9361 ttaaattgga ttaagaagta ataaactttt atgaatcaag gtcaacgtat aaaaaattca
     9421 aatatgaacg gctatcaaca acttaattaa ttgattatta aaaatttata ttgcaccttc
     9481 aaaattgttt atttttaaat ccagcattgt cactagaaaa aagtattatg gctcaaaatg
     9541 aaaagtatat aatatcacat taatatttat cgattatcaa ggctgtagat taatcttgtt
     9601 tggcgagtcg aaaattgaca agtttgtgta ttaccaggta taagaatttg gttgttaatc
     9661 aagagtttgg agctttgagt agaatacgtt tcatgaaacg tctgagtaca gaagcaattt
     9721 cttcctttga aagattcttt tttgatggat gttgcgaacg catttttctt tatctttact
     9781 aaacttttga gctggaatta tttgaataag gaatcaatct taaccagata aaaaaattat
     9841 tatgaaatga aacattataa attccttcta tttgcctttg ttctgcttct tgctttgatt
     9901 tcgtatgtga gttgtcgtgg ctggcaggtt ttaacattga ttcattcaac tgtggggcga
     9961 aaaagctatt taattgtctg tatccttttg actatctgcc tattttttgg gatgtttttt
    10021 gaggacaagt tgtctatttc attagccaga cctctccttt ttttgggtta ttctttttta
    10081 ctattggtaa cttatttgtt cttctcattt ttgttagttg acatttttcg cggactgaat
    10141 cattttctgc attttatttc agatcaagga atgatagctt tgcgattttg gacaatgatg
    10201 gcaagtttgg ttttgattat ggtattaatg atagctggaa atattaaatt caatcatccc
    10261 gctttagtaa atttatattt aaagtcaaat aagtctgttc aaaataagga aataaaaatt
    10321 gttgcagtaa gtgatcttca tttaggaact tgcattgata aaaagcaatt acaaaaatat
    10381 ataacgctta tcaatgcaca acatcccgat ctggtcctga tggtaggtga tatttgtgat
    10441 cgttctctgc cagcaatggt tcatcaaaat ttaaaagaag acttacaaaa aattcaagct
    10501 ccattgggtg tttatgcagt ggatggaaat catgaatatt attctttcca tttagcagaa
    10561 gtaaatgaat attataaaaa tgcaggagtg aatgttttac gtgatagtac cgtagaaatt
    10621 cagcaccagt tttatctggt tgggagagat gattacacca atcgacaacg aaagaaactt
    10681 tctgagttga tggattcttt tgacccttcg aaaccagtca tagtagttga tcatcagccg
    10741 atgaacctgg aagaatcaca agaggaaaat attgaccttc atttatcagg acatacccat
    10801 aatggtcagg tttttccggg caatttgctg gcaaagcgat ttttcgaagt gagttatggt
    10861 tataagaaaa aaaggaatac acatatttat gtaagttcgg gactggggtt atggggacct
    10921 tcttatcgaa ttggtactca atcagaactg gtagcaattc attttcaata ctaaagaaaa
    10981 aataaaaaat tcatctgtaa ttttaaaaat aaaaactccc tttgaaagag tatattcaaa
    11041 aggagtttta ccttaaaaat acctaattgt acaatttttt aaaacctcaa ttttcacgat
    11101 aaattatttt ccttttgtgg taggcgttct tttcctgttt ttcatttgtt cagaacgaat
    11161 agaatcatac ttctgaaatt tttcttttcc aatgattttt tccagttcgg catcattggc
    11221 tttacgatct ttttgcattt gagctcgcat ttcttcacga tttctctgca tctcttcgcg
    11281 atacttcgct ctgttttcat cttccttctt gaaaagctcc gtaactttag ctttttcttc
    11341 gtcagttaaa tttaactcct ttgcccaata attagctcgt tcctcagcac tcattctttt
    11401 tggaggattt tgtctcatct cctttttcat ctgctgaggt tgattttctt tttgtgcaaa
    11461 tatagtgaca ctcaatgtaa aaagtgtaca caacattaca attaaagtct ttttcatacg
    11521 cttgttgttt ttaaaaattt tattttttct attaagactt ttttgaaagg aaagagttta
    11581 aagctttact ttaaatttag aaagattaac aaagatgctg atttagagaa aaaaataaag
    11641 acagacttca ttgcaaaatc tgtctttata ttttcatcat tttttaatac aattactctt
    11701 ccaacaacat ttccataatg gtgcaagctg cttttgcgac agaagtaccg ggtccaaaaa
    11761 tagctgccac acctgctttg taaagataat cataatcctg tggtggaatg acacctccag
    11821 caataaccat gatatcttca cgtccaagtt tttttaattc ctcaattact tgtggaataa
    11881 gcgttttatg tcctccagcc aatgaagaaa tgcccagtac gtgaacatcg ttttctacag
    11941 cctgtttggc tacttcttca ggtgtctgga acaaaggacc catgtccacg tcgaagccac
    12001 agtctgcata accgctggct actactttgg ctccacggtc gtgaccatcc tgtcccattt
    12061 tagcaatcat aatgcgagga cgacgacctt ctttctttgc gaatttttct gttaattcgc
    12121 atgcacgttt gaaatctgca ttttcttttg tttctgatga atacacgcct gatattgttc
    12181 taatggttgc tttataacga cctgctactt tttcgcaggc ataagatatt tctcctaagg
    12241 tagctctgac gcgagcagct tctactgcaa gttccagtaa atttccatta ccggtgcgag
    12301 cacattcagt aatggcatcc agtgcttttt gaacttcttc gttattgcga gtggctcgaa
    12361 gttgttttaa tcgttcaatt tgttgttcgc gtacagcagt attatcaatt tcaagaattt
    12421 caattggttc ttctttttca agacaataac gatttacccc aacaataact tgttcccctg
    12481 aatctatgcg agcttgtgtc ctggcagcag cttcttcaat tttcattttc ggaatgcctg
    12541 tttcaatagc tgctgccatt cctcccagtt cttcaatttc ttgaatcagg ctccaggctt
    12601 tctcactgat ttctttggtg agcgattcta cataataaga acctgcccac ggatcaatct
    12661 gacgacaaat ataagtttct tcctgtaaat aaatttgtgt gtttctggca attcgtgctg
    12721 aaaaatcagt aggaagtgca atagcttcat ccagtgcatt ggtatgaaga gattgtgtat
    12781 gtcccaatgc cgctgccatt gcttctatac aggtacgacc gacattattg aacgggtctt
    12841 gtgcagtaag cgaccagcct gaagtttggg aatgtgtacg aagtgccatt gatttggggt
    12901 tctttggatt aaactgtttg acaatttttg cccacaatag tcgagcagct ctcattttgg
    12961 caatttccat aaaatgattc attcctattc cccagaagaa tgataatcgc ggtgcaaaag
    13021 catcaatatc cagacctgca ttgagccctg tacgaagata ttccagtcca tcagccagcg
    13081 tataagctaa ctctatatcg gctgtagcac cagcttcctg catgtgatag cccgaaatag
    13141 aaatggagtt gaatttgggc atatttttcg aggtatattt gaatatgtca gcaatgatac
    13201 gcattgaaaa ttgaggagga tatatataag tatttcgtac cataaattct ttcaaaatat
    13261 cattttgaat ggtgcctgaa agttcttcca atttggctcc ttgttcttgg gcagctacta
    13321 tataaaatgc aagaataggc aatacagctc cattcatggt catagaaacg gacattttat
    13381 ttaaaggaat tccatcgaaa agaattttca tatcttctac cgaacaaatg gaaacacctg
    13441 ctttacccac atcgcctaca actctttcat gatcggcatc ataacctctg tgagtaggta
    13501 aatcgaaagc aacagaaagt cctttttgtc cagcagccaa gttccgacga taaaaagcat
    13561 tagattcttc tgccgtagaa aaacctgcat attgccgaat agtccacgga cgggttacat
    13621 acatcgtgct atatggacca cgcaaaaaag gaggaattcc tgctgcgtaa ttcaaatgtt
    13681 gtattccttt ttcatcttct ttcgtataaa aagattttac gggaatttgt tcagcagtca
    13741 tccaggtact tacctcaact gcttcctcat ttgaagtagg aataatgttt tgtacatcaa
    13801 ttttactaaa atctggtttc atatttttaa attttagaat tgagagaaac aaattttttc
    13861 aaaaaatatc tgagaattat ttcaaaaacc ttttgctttc tcaattcatt ataaacatgt
    13921 ttttctattt attatttagt tgtcatttta ttatttttca aaaaatcaag aaatagggaa
    13981 caaaataaac acagatacat cccacaaagc atgagaaaca atcaatgtta ctaaattttt
    14041 attgtattta tacaagagtc cccaaaaaat accacaaacc aatgctgata aaataagcat
    14101 aaaattaaat gaccacaaat gaataaaagt gtaagtaata gccgtgatgg caaaagcact
    14161 ccattcacca tattgtggtt ccaacatccg ttgaacataa cctcgccaga aaatttcttc
    14221 agcagggcca attaaaaaag cgagcaaaag tcctaaatat aaagtatttt ctccactttt
    14281 tatcaagtat atatttccaa cttgttcttc tgcaaagaca aaaatcaatt ttgaaaacac
    14341 ataacccaac tggaaaattc catataaaac tatagctgaa ccaataccaa taaatatgtc
    14401 tttaatggaa aatgaaaatt gttttttgaa atttttacca aaaaaagcac taatgaaaat
    14461 aaaaaataca gcagcacatg acatcactat ccagaaatta atgtaacttc tggtccaggg
    14521 tgaaaacata aagaaccaga ggactaatgc aattaaaata ggtaaaacaa cttttttcat
    14581 aaccagtttc ctatcatatt acaaatcgaa aatatcaatc caaaaagcaa cacaagttga
    14641 gccgattttg catccagatc tttgattaat tcaggttttt ctacttgtgc tgtttgcatc
    14701 tgacgaatat tttttatggt caaagggaca gttaaaaata ccagcaatac tatgggatga
    14761 atcattctca aaattattag cagtgctatc atcacataag ctcccactgc cagcgtaacg
    14821 tattgaattt tagctctttt caatcctaaa accattgact gtgttttgat atgggcttta
    14881 ttgtcatcac gaatatcgcg agtattgttg gcatgcaaaa tatttactac cagaaaagca
    14941 atcgggatat taatcaaaat aattttccac agcagttgtt gcgtcattac ataagcggtt
    15001 cctaaaccaa tcaacggacc atagataata aaaataatca aatcacccaa tgctacatat
    15061 ttcaatttag aatatagtac tgctgccaat gtgccaataa taccaatcca gagcaaagca
    15121 tttccagttt tataccagag aaataagccc agcaaaactc cgatgcccag caaaacaaaa
    15181 ccataagaaa gaataaattt cggcgtaaaa actttttcaa ccaataaccg gctagaaccg
    15241 aaagattcag gacgatctac ctgatattta taatcaaaat aatcattgat taaattactg
    15301 ctggcttgaa aaatggctgc tccaataatt gctataatac cataccacca gttgatattt
    15361 ccactgattt cttcattgat ataaaaaata taagtgagag ttattaatgc tggcatggta
    15421 gaagcaggga aagaccacgg ccgcgtagca ataaaataat tgttgagttt tgacttcatt
    15481 tacatcattt catttttgaa aaattctttc attaaatacc caacttttca ttgaaacttt
    15541 ttaaaatttc cagaacattg gattttacac taatgaaatt ttctatacct tgtgttttta
    15601 aatcatccat gcaggctgga gcacccgcta ccacaaaaat cgctttgtct tttactaatt
    15661 gataagctgc tggggcaaat tgtgcatatt cttcatcgct tgaacataga accacaatat
    15721 ccgctttagc ttccaatgca gcctgtacac cttcttccac tgtatcaaat ccgacattgt
    15781 ctatcacttc ataaccagca caggcaaaga aattgcagga aaattgtgaa cgagctactc
    15841 gcatagccag atttccaatg gttagcataa aaacttttgg cctttttgct gcacgctcgg
    15901 tagcaagacg taacgtttca aattctgctg cacctcgaac aggtggtaag gtttctactt
    15961 cagtagattg attgttttta tcacattgcg ggcaattaac cagtttttct gatgctgttt
    16021 cattaaagtt aggatactga ttggttccaa ccaatatttc ttttcgattg gaaatatttt
    16081 tcattcggta attggcggta gctttgatgg cttgttgaac tttgccagca taaatagctt
    16141 ctaaaaagcc tccttcgttt tcaatctcga ggaacaattt ccaggcttgt tgagcaattt
    16201 ctttggtgag gatttcaaga tagtaagaac ctgctgctgg atcaaccact ttatcaaaat
    16261 gagattcttc tttgagtaat agttgttgat ttctggcgat acgttcagca aattcgtttg
    16321 gagcattaaa tacatcatca taacccagaa cagtcaatga atcaattcca ccgagcacag
    16381 cactcatggc ttctgtttgg gtgcgaagca tatttacatg agcatcaaat attgttttat
    16441 tgaaattagt cgtttctgca tgaattctca ttttagtagc acatttacaa gtaccgtcct
    16501 ttgcatttga attatcttca caagaacata caggttgata agcatccaca ataagtgccc
    16561 acagaagtcg agcagctctg aattttgcaa tttccataaa ataattgctg cctattccga
    16621 aattaaattt aattttttta gcagccagcg aagtatcaac tccagcatcc accatcatgg
    16681 aaagatattg atttccccag gccagagcat tgcctaattc ctgtgtaata aaagctccgg
    16741 catttttcaa aattgtagca tttaccgtca ttacacgata aaaaggtaat tttgaaatgg
    16801 catcaatgac tgattttaac tgttcaacaa tttgttcctt gcttttcttt ttgccatctt
    16861 gcaacatttc accaatagga tcaaattcaa tagcaccctg tagattcttt acgtcatatt
    16921 gctggctcag caaatattca gatagagtat tggctaatgt tatggcattg tttgcatcga
    16981 caagaaaatt tagttcgatg cattcagcat taatattttc caataaat
//
LOCUS       DAOSAS010000024        17022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_2325_length_17022_cov_11.897094, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000024 DAOSAS010000000
VERSION     DAOSAS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17022
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2325_length_17022_cov_11.897094"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1511)
                     /locus_tag="PLR72_02045"
     CDS             complement(108..1511)
                     /locus_tag="PLR72_02045"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK36025.1"
                     /translation="MKIKFIGAAREVTGSKHLITTDEGKKILLDCGMFQGKGLETDAK
                     NRHLGFNPAKLDHIILSHAHIDHSGLIPFLYKNGFRGSIICTHATRDLCSIMLTDSAH
                     IQELDVKWFNKKRARQGLPPVDPIYNVEDAENCMDLFIGVAYNRRFYINDKISVKFTN
                     TGHLLGSAVVNLRIIENDRRINLAYTSDIGRPYNHILRPPDPFLPCDYLITESTYGDR
                     LHPDPQQSEEELLKIVWETCVLNKGKLIIPAFSIGRTQEIVFMLNNFYNKGLLPKIEI
                     FVDSPLSVNATDIYRLYSESLNKEVKKVLKYDNDPFGFNSLHYIKNVDESKALNTYKK
                     PCIIISASGMLEAGRVKHHVANNIENPRNTILIVGYCAPTTLGARLQQPGLREISIFG
                     QMHKVKARIAKIEALSGHGDYKEMISFLKCQSPYQIKKTFLVHGDYETQVFYREELKK
                     VGFRRVEIPDIEQEFIL"
     gene            1821..3002
                     /locus_tag="PLR72_02050"
     CDS             1821..3002
                     /locus_tag="PLR72_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK36026.1"
                     /translation="METFFRTHQYLVEHVQSPVKRLLMDEIDWSKRLIGIKGSRGVGK
                     TTFLLQYAKEKFKKDDNSCLYINFNNFYFTIHSLIEFAEWFVQQGGRTLLIDQTFKYE
                     NWSKELRECYDRFPELKIVFSGSSVMRLKEDNPDLQDVVSTYNLRGFSFREFLNLQEG
                     VNFPAYTLDEILKNHIQICKEIVQQIHPLQYFQDYLHHGYYPFFLEHRNFSENLLKTM
                     NMMLEVDILLIKQMELKYLSKIRKLLYLLMINSPVTPNISQLSSEIQTSRATVMNYIK
                     YLKDARLLNLLYPAGDQFPKKPHLVYGHNTNLLHAVMPGNVDKHIEEKTFLYNSLHSK
                     HKVNAGKYHADFIIDEKYFVKCDQKMHSSKNHSKIIYAANGIEIGKKNEIPLWLFGFL
                     Y"
     gene            3109..6636
                     /gene="nifJ"
                     /locus_tag="PLR72_02055"
     CDS             3109..6636
                     /gene="nifJ"
                     /locus_tag="PLR72_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOK36027.1"
                     /translation="MAKTKKFLTCDGNQAAAHIAYMFSEIAAIYPITPSSPMAEYIDE
                     WAAYGRKNIFNETVQVQEMQSEAGAAGAMHGALQAGALASTFTASQGLLLMIPNMYKM
                     SGELLPGVFHVSARSLATHALSIFGDHQDVMAVRQTGCALFATGSVQEIMDLAPVAHL
                     SAIKSRIPFVHFFDGFRTSHEVQKIEQLDKEDLIPLIDQEALKAFRQRALNPNQPVTR
                     GTAQNPDIYFQATEAANPFYETVPEIVEDYLNKLAEITGRKYHLFDYYGAEDADRVVI
                     AMGSSTEAIREGVDYLMAKGEKVGLISVHLYRPFSAKHFLAVLPKTVKRISVLDRTKE
                     PGANGEPLYLDVKNALYGQKDAPIVVGGRYGLSSKDFTPAQVLAVFENLALPEPKNHF
                     TVGIVDDVTFTSLPLREEISLGGEGIFEAKFYGIGSDGTVGANKNSIKIIGENTNKYC
                     QAYFAYDSKKSGGFTCSHLRFGNHPIRSTYLVTTPDFVACHVQAYLKLYDVTKGLKKN
                     GTFLLNTVWNEEEVKKNLPNKVKRYLAKNNINFYIINATNIAQEIGLGNRTNTILQSA
                     FFKISNVIPYELAVEKMKEMIDKSYGKKGQEIVKKNYAAVDRGTEFIKIEVPTEWATL
                     PDDEEIEDNAPEFIKKVVRPMNAQAGDDLPVSVFKGREDGTWEPGTSKYEKRGVATMV
                     PLWNPANCTQCNECAYVCPHATIRPFILDETEQAKATFETIKANGKQFVGMTYRIQVD
                     VLDCMGCNNCADICPGTRGNKALTMVPIETQYDNQKNWDFCINEVKSKQHLVDVKMNV
                     KNSQLATPLFEFSGACAGCGETPYVKLITQLFGDRELVANATGCSSIYSASAPSTPYT
                     TNKNGQGPAWANSLFEDNAEFGLGMVFANEIMRNRLVKIMTEAINDNSCSNELKALFA
                     EWLDNREDVEKTKELASKIVPFVNTCNGKYCKEIAELSSFLVKRSQWIIGGDGWAYDI
                     GFGGLDHAIASGKNFNILVLDTEVYSNTGGQSSKATPMGAVAKFAASGKRIRKKDLGM
                     MAMAYGYVYVAQIAMGANKAQTLRALREAEAYNGPSLIIAYSPCISHGIYAGMEKAQE
                     EQARAVECGYWQLYRYNPMLEEEGQNPFILDSKEPQWDKFQDFLMGEIRYASLMKQYP
                     EEAKELFLAAEKNAKWRYNNYKQMAERQF"
     gene            6811..7380
                     /gene="efp"
                     /locus_tag="PLR72_02060"
     CDS             6811..7380
                     /gene="efp"
                     /locus_tag="PLR72_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOK36028.1"
                     /translation="MATTADIKNGMCINLDGQFYIITEFLHVKPGKGPAFVRTKMKNV
                     VTGRTFEKTFSVGTHIDEVRIERRKYQYLYQDDMGYYFMNNETFDQVCINKEMINGVD
                     FLKEGMICDVVVHAESETILYVDLPIQVTLTVTYTEPGLKGDTATNATKPATLETGAN
                     IRVPLFINTGDTIRVDTRDGSYVERVKTE"
     gene            7484..7849
                     /locus_tag="PLR72_02065"
     CDS             7484..7849
                     /locus_tag="PLR72_02065"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOK36029.1"
                     /translation="MKIKEFLKSFVYAAEGIKVMFACGRNMKVNLFTVFVVIVLGIIF
                     HISAFEWISCLLCFGLVISLEMINTSFENLVNLVSPDFHPLAKKAKDIAAGAVLVSAI
                     IAFIIGLIIFLPKIIDAIS"
     gene            7872..8564
                     /locus_tag="PLR72_02070"
     CDS             7872..8564
                     /locus_tag="PLR72_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOK36030.1"
                     /translation="MLKCNFLTDFINLLYPNLCLICGESLMKNEEQLCSKCLYQIPKT
                     NYHLIKNNLIEKRFWGKVPIEQATSFFFFQKGSPFQKLLHQLKYHGNKELGEILGKYA
                     AADLILTDYFSAVDMIVPVPLHPKKYNQRGYNQSEWIGKGLSTILQKPQIIDNLVRIK
                     ESTTQTKKSVYERYENTEGIFSVKDENFFSNHHILLVDDVLTTGSTLEACAHALLTAE
                     NVQISVFTLAVA"
     gene            8613..9140
                     /locus_tag="PLR72_02075"
     CDS             8613..9140
                     /locus_tag="PLR72_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOK36031.1"
                     /translation="MENRIEIVKGDITKEEVDAIVNAANRSLLGGGGVDGAIHRAAGP
                     ELLEECKKMKGCETGEAKITKGYRLPAKYVIHTVGPVWNGGNYHERELLANCYKNSLK
                     LGQQYGIKTIAFPSISTGAYRFPIELASKIALNEIYQFLKNNTSIEKVKIVCFDNQTY
                     ETYQRNLEELMKNER"
     gene            9313..10059
                     /gene="fabG"
                     /locus_tag="PLR72_02080"
     CDS             9313..10059
                     /gene="fabG"
                     /locus_tag="PLR72_02080"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOK36032.1"
                     /translation="MKLLEGKVAIITGAARGIGKAIALKFASEGADIAFTDLIIDENA
                     LNTQKEIASFGVKVKGYASNAANFEETHAVVEEILKEFGHIDILVNNAGITKDGLIIR
                     MSEAQWDAVINVNLKSAFNFIHACAPIMMKQKAGCIVNMSSVVGVSGNAGQANYSASK
                     AGLIGLTKSVAKELGSRGIRANAVAPGFIETEMTHALSDEQRAKWVENIPLRRGGTPE
                     EVAKVTLFLASDLSSYVNGQVIHVCGGMNT"
     gene            complement(10127..11242)
                     /locus_tag="PLR72_02085"
     CDS             complement(10127..11242)
                     /locus_tag="PLR72_02085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK36033.1"
                     /translation="MQKTAIVCVTNDLTTDQRVHKTCLTLQKCGYQTIEVGRMLPQSL
                     PLERPYSVHRFKLWFRKGPQFYAEYNIRLFFYLLFSKVDLIFANDLDTLPAAFLAAKI
                     RRKRLIYDTHEYFTETPELVNRPRTKKIWEKIENFIFPKLTDIITVNQSIANLYESKY
                     HKKIYVCRNIPLDFTPTRLKTREELGLPLDKHILILQGTGINVQRGAEEAVLTMQYLE
                     DCVLLIIGSGDIIPELQKMIDQYQLQEKIIIKPKMLFQELRQYTMNSDLGLAIDKDTN
                     LNYHYSLPNKLFDYIHAEIPILSSGLPEIKQIIDQYDIGYYIQNHHPKHIAEVIQNIF
                     NNPQRYALVKQNTAKAKAELNWEKEEQILIELINKTF"
     gene            complement(11244..12572)
                     /locus_tag="PLR72_02090"
     CDS             complement(11244..12572)
                     /locus_tag="PLR72_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOK36034.1"
                     /translation="MKMQEVIEYIIRFLLGDESSTEIANLIGYTSDKEEFSRYKLVIY
                     PSDFFQPEVYGTPQTLPVLPLKIWEDTPILFGQPKIEKIGQTRILYADIIASTYFLIT
                     RYEEMIRPECRDVHGRFPGKESISYRAGFIDRPLVDEYGKLLRKQLRELGIDVPEPSK
                     KIKKIFLTHDVDQLAHYRRFRGMMGALWRGIKQPNKRPEAKRAIKTFFKGIRFDPWYT
                     FPWLFKMDLSLIDILGKERCEIIVFIKAGGGKQREDKPHSNLVHPDYQNLIKYCKRKN
                     ITIGLHTSYKAGKYPELIAEEKKRLERNAKVKIYYNRNHFLRMCEPEDMETLIQAGIT
                     DDFTLTYADVAGFRIGTCRPVKWINPKNKELTSLTLHPMTIMDSTLSDKRYMNMSAYD
                     AYEYCVQLINNIENYGGEFVLLWHNTMVEEKSQSYHRTLYQNIIDYLKTK"
     gene            complement(12629..13861)
                     /locus_tag="PLR72_02095"
     CDS             complement(12629..13861)
                     /locus_tag="PLR72_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOK36035.1"
                     /translation="MILNYIWIGFFLIAFVVAFIQMIVYGNVQVFSDMLNATFDSAKT
                     GFEISLGLTGVLSLWLGIMKVGERGGVVQAFSKVVSPFFEKIFPDLPKNHPAYGSMLM
                     NIAANMLGLDNAATPLGLKAMKEMQETNPKKDTASNPMIMFLVLNTSGLTLIPVTIMV
                     YRAQLGAANPSDIFLPILIATFFSTLAGFLSVAAYQKINLLDKTILKTLLGLIIIIGS
                     ILYFFSKVTQNQLTVYSNFIASFILISLIISFIVAAMVHKINVYDAFIEGAKEGFQVA
                     IGIIPYLIAILVAIGMFRAAGAMDMLMNGFAWFFGLIGVNTDFVGALPTALMKPLSGS
                     GARGMMVDAMKMYGADSFVGRLSCIIQASTDTTFYILAVYFGSVGIKNTRYAVICGLL
                     ADFVGIVAAILIGYLFFH"
     gene            14225..14923
                     /locus_tag="PLR72_02100"
     CDS             14225..14923
                     /locus_tag="PLR72_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase"
                     /protein_id="HOK36036.1"
                     /translation="MGKIKDEKQQEIVKTISEGRTRTNLLRKYEQSTLAYLVQRIPAW
                     MSSDMLTAIGFFGSLIVFLSFVLAAYVNRYLLLIGVLGFVISWFGDSLDGRVAYYRHK
                     PRKWYGFSLDITVDWLGVILMGAGFIVYAISPWKIIGFLFVGLYGWEMITALLRYKII
                     NQYSIDSGAFGPTEVRILISLILVLEVIFKNSIIYTSSLACLILLIMNILETKKILQL
                     ADERDKNERGGEKI"
     gene            14928..15347
                     /locus_tag="PLR72_02105"
     CDS             14928..15347
                     /locus_tag="PLR72_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOK36037.1"
                     /translation="MIERLFTFTKAQFSAMLGGAIDYTTMVFFTEVIHIHYTVSIVIG
                     GIVGALVNFTVNKGWTFRSKELPYKSTGLKQLLKFSLVVANSIFLKDVGTYLITTFVK
                     IDYKFSKIIVDLIVSIGFNYNLQKYWVFKKVPVNTTN"
     gene            complement(15428..15946)
                     /locus_tag="PLR72_02110"
     CDS             complement(15428..15946)
                     /locus_tag="PLR72_02110"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOK36038.1"
                     /translation="MKKFAILLLCLLPLKLIGQELKLGHINSQEILSLMPERSQIEKT
                     LGDLQNQWENELLKMQQEYYAKIKEFQDKQATMPESIKQARQSEIVEMEQRITTFRQT
                     ADSDLMKKQQELFAPVIEKVKKAINEVGAENNYTYIFDISTQVIVYQSPKSNDLTPLV
                     KKKLGLSSSSIK"
     gene            complement(16124..16840)
                     /locus_tag="PLR72_02115"
     CDS             complement(16124..16840)
                     /locus_tag="PLR72_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36039.1"
                     /translation="MKKYLALLLFALITTMSFGQSNYQDVVYLKNGSIIRGVIIEQVP
                     NKSIKIETADRSVFVYQMDEIEKLTKEPIQGKSGGSFSNSGLQSGYKGIVELGYQIGT
                     GDYGMDRLKLNIINGYQLNPYFSLGLGTGLRYYFDAEAALIPIFADFRANFMDNNVSP
                     YLSLGVGYSFDATNSFEGVGFLLNPTIGVSFKVSEKSAMNVGLGYEMQRMKFFYYDWY
                     SIDEFTENSGAISINVGISF"
BASE COUNT         5697 a         2612 c         2865 g         5848 t
ORIGIN      
        1 aagccccaaa acgttttccc aatattaccg cacgattgta tcgtgtggaa gatctttttt
       61 aatcaaaaga attcctcgag gcttgcctcg gggttattct tttgaattta taaaataaat
      121 tcctgttcaa tgtctgggat ttctacacga cgaaaaccta cttttttcaa ttcttctcta
      181 taaaatactt gagtctcgta atcgccatgg accaggaaag tttttttaat ttggtatggc
      241 gactgacatt tcagaaaaga aatcatttct ttataatctc catgtcccga aagagcctct
      301 attttagcaa ttcgagcttt gaccttatgc atttgtccga aaatagaaat ttctcttaaa
      361 cctggttgtt gtaagcgtgc tcccaacgtt gttggtgcac aataaccaac aattaaaata
      421 gtatttcgtg gattttcaat gttattggca acgtgatgct ttacacgtcc tgcttctaac
      481 attcctgaag cagaaattat aatacacggt tttttataag tattcaaagc ctttgattca
      541 tcaacatttt taatataatg aagagaatta aaaccaaaag gatcgttgtc atatttaaga
      601 actttcttta cttctttatt aagtgattca ctataaaggc gataaatatc agtagcatta
      661 accgaaagtg ggctatcgac gaaaatttca attttaggta aaagtccttt attgtaaaag
      721 ttatttaaca taaaaacaat ttcctgtgtt cgtccaatag aaaatgctgg aataattaat
      781 tttcctttgt ttaaaacaca agtttcccat acaattttca acaattcttc ttcgctttgt
      841 tgaggatcag gatgtagcct atcgccataa gttgattcgg taatcaggta atcacaaggt
      901 aaaaaaggat caggtggacg aagaatatga ttataggggc gtccaatatc actcgtataa
      961 gcgagattta ttcttctatc attttcgata attcttaggt ttacaacagc acttcctaat
     1021 aaatgacctg tattggtaaa tttgacacta attttatcat taatataaaa tcttctattg
     1081 taagcgactc caataaacaa atccatacaa ttttcggcat cttcaacatt ataaattgga
     1141 tcaacgggag gcaatccttg tcgggctcgt tttttattaa accattttac atcaagttcc
     1201 tgtatgtgag cactatcagt caacataata gaacaaaggt cacgtgtggc gtgtgtacaa
     1261 ataatggagc ctcgaaagcc atttttatat aaaaaaggaa ttaatccaga atgatcaata
     1321 tgagcgtgag aaagaataat gtgatctaat tttgcaggat taaatccaag atggcggttt
     1381 ttagcatctg tttcaagacc ttttccttga aacattccac aatccagtaa aatttttttt
     1441 ccttcgtcgg tagtaattaa atgtttactg cctgttacct cacgtgcagc tccaataaac
     1501 ttaattttca tagttgtttg ttttttctta atatcgcaaa taattccctc ataattactt
     1561 gtttatatat aacacgaaag gaattataat aatgtttatt agttttcaaa acaggagata
     1621 aattataatt ttctttggtt aaaaatgaat cattttcaaa agtagcataa tatttttatt
     1681 tttttctttt ttatgagaaa aagttaaaca agttactaat ttttcataga ataaaatcat
     1741 gctaaaaatt atgattttga catctcgact ttgcaaaatg aatttatttt ttgtattttt
     1801 gcataccaat atacatccca atggaaacat tttttcgtac tcatcaatat ttagtggagc
     1861 acgttcaatc gcctgtaaaa aggttattga tggatgaaat tgattggtca aaacgtttga
     1921 ttggaataaa aggtagccgt ggggtaggga agacgacttt tcttttgcaa tatgcaaaag
     1981 aaaagtttaa aaaagatgac aatagttgtc tttatattaa ttttaataat ttttacttta
     2041 ctattcattc cttaatcgaa tttgctgaat ggtttgtgca acaaggagga aggacattac
     2101 tgattgatca aacttttaag tatgaaaatt ggtctaaaga attacgggaa tgctacgatc
     2161 gctttccgga attaaaaatt gtcttttctg gttcatctgt tatgcgatta aaagaagaca
     2221 accctgacct gcaagatgtg gtaagtactt ataatttaag aggtttttca tttcgtgagt
     2281 ttttaaatct tcaagaagga gtaaatttcc cagcttatac actcgatgaa atattaaaaa
     2341 atcatattca aatttgtaaa gaaatagttc agcaaattca tcctttgcaa tattttcagg
     2401 attatttgca tcatggttat tatccttttt ttctcgagca taggaatttc tctgaaaatt
     2461 tattgaaaac aatgaatatg atgctcgaag tagatatttt actgataaag cagatggaat
     2521 taaaatattt gtcaaaaatt cgtaaattgc tgtatttact tatgataaat tcccctgtta
     2581 ctcctaatat tagtcaatta agtagtgaga ttcaaacttc aagagctacc gtaatgaatt
     2641 atataaaata tttaaaggat gcccgcttac ttaatttgct ttatcctgca ggagatcaat
     2701 ttcctaaaaa gcctcatctg gtttatggac ataatacgaa tttgcttcat gcggtaatgc
     2761 ccggtaatgt tgataaacat atagaagaaa aaactttttt atataattct ttacattcta
     2821 agcataaagt aaatgcaggg aaatatcatg cggattttat aattgatgaa aaatattttg
     2881 ttaaatgtga tcagaaaatg cattcttcaa aaaatcattc aaaaattatt tatgctgcta
     2941 atgggattga aataggtaaa aaaaacgaaa ttcccttatg gttgtttggt tttctttatt
     3001 gatcaatgag ataatcttta atcataaata aataaaattt tttaaaataa aattcatata
     3061 tttgtttaat tttcaaataa ttaataatca ttcaataact ataataaaat ggctaaaaca
     3121 aagaaatttt taacttgcga tggtaatcaa gcagcagctc atatagctta tatgtttagt
     3181 gaaatagctg ctatttatcc tattactcct tcctcaccta tggcggaata tatagatgaa
     3241 tgggcagcct atggtcgaaa aaatattttt aatgaaacgg tgcaagttca agaaatgcaa
     3301 tctgaagcag gtgctgctgg agctatgcac ggggctcttc aagcaggagc attggcaagt
     3361 acttttacag cttcacaggg tttgcttttg atgattccta acatgtataa aatgtctggt
     3421 gagttattgc cgggtgtttt tcatgtgtca gcacgatctc ttgctactca tgctctttct
     3481 atttttggcg atcatcagga tgtaatggca gttcgtcaaa cgggttgtgc tctttttgca
     3541 actggttcag ttcaagaaat tatggattta gctcctgttg cacatttatc agcaataaaa
     3601 tctcgtattc cttttgtaca tttttttgat ggtttccgta cttcccacga agttcaaaaa
     3661 atagaacaat tggataaaga agatctaatt cctttaattg atcaagaagc tttgaaggct
     3721 tttagacaaa gagctttaaa tcctaatcaa ccggtaactc gaggtacagc tcaaaatcct
     3781 gatatttatt ttcaagctac cgaagctgct aatccttttt acgaaacagt acctgaaatt
     3841 gtagaagatt atcttaataa attagctgaa ataacaggaa gaaaatatca tcttttcgat
     3901 tattatggtg cagaagatgc tgatcgtgtt gtaattgcaa tgggttcttc aaccgaagct
     3961 attcgagaag gagttgacta tttgatggct aagggtgaaa aagttggttt gatttctgtg
     4021 catctttatc gtcctttttc tgcaaaacat tttttagctg ttcttcctaa aactgtaaaa
     4081 agaatcagtg ttttagatcg tacaaaagaa cccggtgcaa atggtgaacc tttatatctg
     4141 gacgtaaaga atgccttata tggacaaaaa gatgctccaa tcgtggtagg tggacgctat
     4201 ggtttgtctt ctaaagattt tactcctgct caagtattag ctgtttttga aaatttagct
     4261 ttaccagagc ccaaaaatca ttttacagta ggaattgttg acgatgttac tttcacttct
     4321 cttccactaa gagaagaaat ttcactgggt ggtgaaggaa tttttgaagc taaattttat
     4381 ggtatcggtt ctgatggaac agtaggagcc aataaaaatt caattaaaat cattggggaa
     4441 aatactaata aatattgtca agcttatttt gcctatgact caaaaaaatc gggaggattt
     4501 acctgttctc atcttcgttt tggcaatcat cctatacgtt caacttattt agttaccaca
     4561 ccagattttg ttgcttgtca tgttcaagct tatttaaagc tttatgatgt aactaaaggg
     4621 ctgaaaaaaa atggaacttt ccttctgaat acggtatgga atgaagaaga agtaaagaaa
     4681 aacttgccta ataaagtaaa aagatattta gcaaaaaata acattaattt ttatattatt
     4741 aatgccacaa acattgctca ggaaattggt ttaggaaatc gcaccaacac tattcttcaa
     4801 tcagctttct ttaaaatttc taatgttatt ccttatgaat tggctgttga aaaaatgaaa
     4861 gaaatgattg ataagtccta tggcaaaaag ggtcaagaaa ttgtaaagaa aaattatgca
     4921 gccgttgatc gaggaacaga attcattaaa atagaagttc caactgaatg ggcaacgtta
     4981 ccagatgatg aagagataga agataatgca cctgaattca ttaaaaaagt ggttcgtccg
     5041 atgaatgctc aggcaggcga tgatttgcca gtatcggttt ttaaagggcg agaagatggc
     5101 acgtgggagc caggtaccag taaatatgaa aaacgaggtg tagcaacaat ggttccccta
     5161 tggaatccag caaattgtac gcaatgcaac gaatgtgctt atgtttgtcc gcatgctact
     5221 attagaccat ttattttaga tgaaacagaa caagcaaaag caacttttga aaccatcaaa
     5281 gctaatggaa aacagtttgt aggaatgact tatcgaattc aagtggatgt gcttgattgt
     5341 atggggtgta ataattgtgc agacatttgt ccaggtacca gaggaaataa agcacttaca
     5401 atggttccta ttgaaacaca atatgataat cagaaaaatt gggatttttg cataaacgaa
     5461 gtcaaatcga aacaacattt ggtagatgta aaaatgaatg taaaaaattc ccaattagct
     5521 actccactgt ttgaattttc aggagcttgt gctggttgtg gtgaaacacc ttatgtgaaa
     5581 ttaattactc agctttttgg tgaccgtgag ttggttgcca acgctactgg ttgcagctct
     5641 atttattctg cttcagctcc ttctactcct tatacgacca ataaaaacgg acaaggtcca
     5701 gcctgggcaa attctttgtt tgaagataat gctgaatttg gactgggaat ggtatttgcc
     5761 aacgaaatta tgcgaaatcg cttggtcaaa ataatgacag aagcaataaa tgataatagc
     5821 tgttccaatg aattgaaagc tttatttgct gaatggctgg ataatcgaga agatgttgaa
     5881 aaaacaaagg aattagcctc taaaattgtt cccttcgtta atacttgtaa tggaaaatac
     5941 tgtaaagaaa tagctgaatt aagttctttt ttagtaaaac gttcgcaatg gattatcgga
     6001 ggcgatggtt gggcttatga tataggtttt ggaggtttag atcacgccat tgcttcagga
     6061 aaaaatttca atattttagt attagatact gaagtttatt ctaatacagg aggacaatct
     6121 tctaaagcta ctccgatggg agcagttgct aaatttgccg cttcgggcaa acgtattcgt
     6181 aaaaaagatt tgggcatgat ggctatggct tacggttatg tttacgtagc tcagattgca
     6241 atgggagcaa ataaagctca aacgctcagg gctttaagag aagctgaagc ttataatgga
     6301 ccatcattaa ttattgctta ttctccttgt attagtcacg gtatttatgc aggcatggaa
     6361 aaagcccaag aagaacaggc acgagctgtt gaatgtggtt actggcagtt gtatcgttat
     6421 aatccaatgc tggaagaaga aggtcaaaat ccatttattt tggattcaaa agaaccacaa
     6481 tgggataaat ttcaagactt tctgatgggc gaaattcgtt atgcttcttt gatgaaacaa
     6541 tatcctgaag aagctaaaga attattcctt gcagccgaaa aaaatgcaaa atggcgttat
     6601 aataactata agcaaatggc tgaaagacaa ttttaagata ataatttatt cttaaataat
     6661 tctttgagtt atcaaaaagg ctattcattt gaatagcttt tttgataata taattctgat
     6721 aatcaattta ttttgaaaat ataaataaaa ttattatttt tgtaaaacaa atctttctca
     6781 ggtaattaaa ttcaaatttt taacaatgat atggcgacaa cagcagatat taaaaatgga
     6841 atgtgtatta atcttgatgg tcaattttat atcatcacag aatttcttca tgtaaaaccg
     6901 ggtaaaggac ctgcttttgt tcgtacgaaa atgaaaaatg tagtgacggg cagaactttt
     6961 gaaaagactt tcagtgtagg aactcacatt gatgaagtac gtatagaacg acgtaagtat
     7021 caatatcttt accaggatga tatgggttat tattttatga acaatgaaac tttcgatcaa
     7081 gtttgcataa acaaagaaat gatcaatggc gtagattttt tgaaagaagg aatgatttgt
     7141 gatgtggtgg tacatgctga atcggaaact attttatatg tggatttacc tattcaggta
     7201 acgttaactg taacttacac agagccaggg cttaaaggag atactgctac taatgccacc
     7261 aaaccagcta ctctcgaaac aggtgccaat attcgcgttc ctctttttat caatacaggg
     7321 gatacaatca gagttgatac tcgtgatggt tcttatgtag aaagagtcaa aactgaatag
     7381 ttaaaaatat tttctcacga tttttttgaa tgatctattt atccgataaa taattaagtt
     7441 tatcggattt tttttatttt tgtgcataga taaaaaaatt gtcatgaaaa taaaagaatt
     7501 tttaaaaagc tttgtctatg ccgctgaagg tataaaagta atgtttgcct gtggaaggaa
     7561 catgaaagtt aatttattta ctgtttttgt agttattgtt ttaggaataa tttttcatat
     7621 cagtgctttt gaatggataa gttgcctatt atgtttcggt ttggtaatca gtctggaaat
     7681 gattaatact tcttttgaaa atttggtcaa tttagtttcg cccgattttc atccgttagc
     7741 aaaaaaagcc aaagatattg cagcaggagc tgttttggtt tcagcaatta ttgcttttat
     7801 tattggatta atcatatttt taccgaaaat tattgatgcc atttcttgaa gcttttaaaa
     7861 aacaatgtaa aatgttaaaa tgtaattttc tgactgattt tattaacctt ttatacccca
     7921 acctatgtct tatttgtgga gaaagtttaa tgaaaaatga ggaacaatta tgttcaaaat
     7981 gtttgtatca gattcctaaa acgaattatc atctcataaa aaataatctt atagaaaaac
     8041 gtttttgggg aaaggttcca attgaacagg ctacttcttt cttctttttt caaaaaggaa
     8101 gtccttttca aaaattattg catcagttga agtatcatgg caacaaagaa ttaggtgaaa
     8161 ttttaggaaa atatgctgct gcagatttga ttttgaccga ttatttttct gctgtcgata
     8221 tgattgtacc agtaccactt catcctaaaa aatacaatca acgtggttat aatcagagtg
     8281 aatggattgg taaaggatta tcaactatcc ttcaaaaacc acaaatcatt gataatttag
     8341 tacgtattaa agaaagtact actcaaacca aaaaatcagt ttacgagcgt tatgaaaata
     8401 cagaaggcat tttttctgtg aaagatgaaa attttttttc aaatcatcat attttattgg
     8461 ttgatgatgt tttaacaaca ggttctactt tggaggcttg tgctcatgca cttttgacag
     8521 ccgaaaatgt tcaaataagc gtttttacat tagcagtggc ttgagtttac caatattttg
     8581 aaaaattatt cacaaattat aaatcatgga atatggaaaa tagaatagaa attgtaaaag
     8641 gcgatattac aaaggaagaa gttgatgcga ttgtcaatgc agccaatcga agtcttttag
     8701 gtggaggtgg tgtggatggt gctattcata gagcagccgg tccagagtta ctggaagaat
     8761 gcaaaaaaat gaagggttgt gaaacgggtg aagcaaaaat tacaaaaggt tatcgcttgc
     8821 cagctaaata tgtgattcat accgttggtc cagtttggaa tggcggcaat tatcacgaaa
     8881 gagaattatt agctaattgc tataaaaaca gtttaaaatt aggacaacaa tatggcatta
     8941 aaacaattgc attcccctct attagtacag gagcttatag attccccatt gaattggcaa
     9001 gcaaaattgc tttgaacgaa atttatcaat ttttaaaaaa taacacttca atcgaaaaag
     9061 taaaaattgt ttgttttgat aatcaaactt atgaaactta tcaacgtaat ttggaagaac
     9121 ttatgaaaaa tgagaggtag aatttttact caattctact ttgtaattat ttatcaagac
     9181 atttatttta ttgattataa atagattgtg tttatattta gaaatttata aattaaaaac
     9241 ttttctcatt tatcaataaa atcttacttt tgtgctgaaa ttttaaaaat taattcatta
     9301 acaaattaac ttatgaaatt attagaagga aaagtagcga ttatcacagg tgctgcacgt
     9361 ggaataggaa aggcaattgc ccttaaattt gccagtgaag gagccgatat tgcttttact
     9421 gatttaatca ttgacgaaaa tgccttgaat acgcaaaaag aaattgcttc ttttggtgta
     9481 aaagtaaagg gttatgcttc caatgctgca aactttgagg aaactcatgc agtagtggaa
     9541 gaaattctca aagaatttgg acatattgat atattggtga ataatgctgg aattaccaaa
     9601 gatggtttaa ttatacgaat gagtgaagca caatgggatg ccgttatcaa tgtaaatctg
     9661 aaatcagctt ttaattttat tcatgcttgt gcacccatta tgatgaaaca aaaagctggt
     9721 tgtattgtta atatgagttc tgttgttggg gtatctggaa atgctggaca agctaattat
     9781 tctgcatcaa aagcaggttt gattggactt accaaatcag tagcaaaaga acttggttct
     9841 cgtggcattc gagcaaatgc agtagcacca ggttttattg aaactgaaat gacccatgct
     9901 ttaagtgacg aacaaagagc aaaatgggtt gaaaatatac ctttacgtag aggtggaacg
     9961 cccgaagaag tagcaaaagt tacacttttt ttagcttctg atttatcaag ctatgtaaat
    10021 ggacaagtaa ttcatgtatg cggtggaatg aatacttaat tatctgataa acaaataatt
    10081 tctaaaaaag cggaacaaaa cttccgcttt tttagttgcg ttaaattcaa aaagttttat
    10141 taataagttc tatcaatatt tgttcttctt tttcccaatt taattctgct tttgctttgg
    10201 cagtattttg tttaacaaga gcataacgtt gtgggttgtt aaaaatattt tgaattactt
    10261 cagcaatatg ttttggatgg tgattttgaa tatagtagcc aatatcatat tgatcaataa
    10321 tttgtttaat ttctggtaaa cctgatgata aaattggaat ttcagcatga atataatcaa
    10381 aaagtttatt gggaagcgaa taatggtaat taagattggt gtctttatca atagccagcc
    10441 ctaaatcgct attcatagta tattgtctga gttcttgaaa aagcattttt ggttttatga
    10501 ttattttttc ctgaagttga tattgatcaa tcatcttttg aagttcggga atgatatctc
    10561 cacttccaat aattaacaac acacaatctt caagatattg catagttaaa acagcttctt
    10621 ccgcaccacg ctgcacatta attcctgttc cttgcaaaat aagaatgtgc ttgtctaagg
    10681 gcaatcccaa ttcttcacga gtttttaatc gagtaggagt gaagtcaagt gggatatttc
    10741 tacaaacata aattttttta tgatattttg attcatatag atttgcaata gattgattta
    10801 cagtgattat atcagttaat ttaggaaaaa tgaaattttc aattttttcc cagatttttt
    10861 ttgtgcgagg tcggtttact aattcaggag tttctgtaaa atattcatgg gtgtcgtaaa
    10921 ttaatctttt tctacgaatt ttcgctgcca aaaatgctgc tggtaatgta tccaaatcat
    10981 tggcaaaaat cagatcaact tttgaaaaga gtaaataaaa aaacaagcga atgttatatt
    11041 ccgcataaaa ttgaggacct ttacggaacc agagtttaaa tctatgaaca gaataaggtc
    11101 tttccaaagg taaactttgg ggcaacattc gaccgacttc aatagtttga taaccacatt
    11161 tttgtaaagt tagacaggtt ttatgaacac gttgatctgt ggttaaatca ttggttacgc
    11221 aaactatggc tgttttttgc attttatttt gttttcagat aatcaataat attttgatat
    11281 aaagtgcgat gataggattg agatttttct tcaaccattg tgttatgcca caataaaaca
    11341 aattctcctc cgtaattttc tatattattg attaattgta cgcaatattc gtaagcatcg
    11401 taagcactca tattcatata gcgtttatca cttaacgtgc tgtccatgat agtcatggga
    11461 tgaagggtca gtgaagttaa ttccttattt ttaggattaa tccattttac gggacgacat
    11521 gttccaattc taaaaccagc cacatcagca taagttaatg taaaatcatc agttattcct
    11581 gcctggataa gtgtttccat atcttctggc tcgcacatcc tcaaaaaatg attacgatta
    11641 tagtaaattt tgactttcgc atttcgctct aatctttttt tctcttctgc aattaattct
    11701 ggatacttac ctgctttgta actggtatgc aagccaatcg taatattttt tcttttacaa
    11761 tattttataa gattttgata gtcaggatga actaaatttg aatgaggttt gtcttccctt
    11821 tgttttcctc caccagcttt tataaaaacg ataatttcac atctttcttt tcccaaaata
    11881 tcaattaatg ataaatccat tttaaaaagc catggaaaag tgtaccacgg gtcaaatctt
    11941 attcctttaa aaaaagtttt tatggctctt ttagcttcag gtcgtttatt gggttgtttg
    12001 attcctctcc ataaagctcc catcattcct ctaaacctac gataatgagc taattggtca
    12061 acatcatgtg ttaaaaatat tttctttatt ttttttgagg gttcaggcac gtctatccct
    12121 aattctctta attgtttccg taaaagtttt ccatattcat caaccaatgg acgatcaata
    12181 aaaccagccc gataactaat ggattctttt ccaggaaagc gtccgtgaac atctcgacat
    12241 tctggccgaa tcatttcttc ataacgtgta attaaaaaat aggtgctggc aattatatct
    12301 gcataaagaa ttcttgtctg tccaattttt tcaatcttcg gctgtccaaa aagaataggt
    12361 gtatcctccc aaatttttaa tggtaaaact ggcaaagttt gaggagttcc ataaacctct
    12421 ggttgaaaaa agtcagaagg ataaataacg agcttataac gactaaattc ttctttatca
    12481 gaagtatatc ctatcaaatt agcaatttca gttgaacttt cgtcacctaa taaaaatctt
    12541 attatatatt caattacttc ttgcattttc ataaaaatta ctttgtctta tgtcgttaac
    12601 tgaaaatcag tttttaaatt aaattttttc agtgaaaaaa taaatatcca attaaaatag
    12661 ctgcaacaat tccaacaaaa tcagctaata aaccacaaat aaccgcataa cgagtatttt
    12721 taatgccaac cgatccaaaa taaacagcca gaatataaaa agtggtatca gtagaagctt
    12781 gaatgatgca ggaaagtctg ccaacaaaag aatcggcacc atacattttc attgcatcta
    12841 ccatcattcc tcttgcacca ctgccactta aaggtttcat taaggcagta ggtaaggctc
    12901 ctacaaagtc agtattaacg cctattagcc caaaaaacca ggcaaaacca ttcattaaca
    12961 tgtccatagc accagcagca cgaaacatgc caattgccac taaaatagca atcaaatatg
    13021 gaataatacc aattgccacc tgaaaacctt cttttgcacc ttcaataaaa gcatcatata
    13081 catttatttt atgaaccatt gcagccacaa tgaaagaaat gatcagagaa attaagataa
    13141 agctggcaat aaagtttgaa tatactgtaa gttgattttg agtaacttta ctgaaaaagt
    13201 acaaaataga accaataata attattaacc ctaataaagt ttttaaaata gttttgtcca
    13261 gtaaattgat tttttgataa gccgccacac ttaaaaatcc tgcaagtgtc gagaaaaagg
    13321 tagctatcaa aataggaaga aaaatatcgg acggattagc tgctcccaat tgggctcgat
    13381 aaaccataat tgttacaggg atcaaagtta atcctgaagt atttaacaca aggaacataa
    13441 tcataggatt ggaagcagta tctttttttg ggttggtttc ctgcatttct ttcatagctt
    13501 ttagtccgag tggagtagct gcattatcca atcccaacat attcgcagca atattcatca
    13561 acatacttcc gtaagctgga tgatttttag gaagatcagg aaaaattttt tcaaaaaaag
    13621 gtgaaactac tttagaaaaa gcttgaacca cgccgccgcg ttcacctact ttcataattc
    13681 ccaaccacaa agaaaggaca cctgttaaac ccaaagaaat ttcaaaacct gtttttgctg
    13741 aatcaaaagt agcattgagc atatcagaaa acacctgaac attcccataa acaatcattt
    13801 gaataaaagc aacaacaaaa gcaatcaaaa aaaatccaat ccatatataa tttaaaatca
    13861 tataattatc tttttggaag aaaattgaat accagtaatg tcatcattaa ttattgttct
    13921 gctgcaaaaa tacattattt tttagttttt accttctctt tcattttatt attgataaca
    13981 aagctaaaaa tcttaatatt tatttgagaa attagattaa caaactatat aaagttagat
    14041 aatttttaga aataatttat tattttttcc aactaattga attacaaaag ttttgatatt
    14101 gtcttatgtt ttatttttta aaattataga ttattgtttc aaaaaattgc atagagttgt
    14161 aaaaaatact acttttgcgg tataagcaac ctcatttaaa aattattctt tccactacat
    14221 aaaaatggga aaaattaaag atgaaaagca acaagaaatt gtcaaaacaa tttctgaagg
    14281 aagaacgcga accaatttat taagaaaata cgaacaaagt acccttgctt atttagttca
    14341 acgcattcca gcctggatga gttctgatat gcttaccgct attggttttt ttggaagtct
    14401 gattgttttt ttaagttttg tattagcagc gtatgtaaat agatatttac ttttaatcgg
    14461 cgtattggga tttgtcatta gttggtttgg cgattcgctt gatggaagag ttgcttatta
    14521 tcgacataaa ccaaggaaat ggtatggttt ttctttagat attaccgttg attggctggg
    14581 tgttatttta atgggtgccg gatttattgt gtatgcaatt tctccctgga aaataatagg
    14641 ttttttgttc gtagggctat atggttggga aatgataaca gctttactac gttacaaaat
    14701 tattaaccaa tattctattg attctggagc atttggacca actgaagtgc gtattttaat
    14761 ttcacttatt cttgttcttg aagtaatttt taaaaattca attatttata cttcttcatt
    14821 ggcatgttta attttattga taatgaatat tttagaaact aaaaaaatat tacaattagc
    14881 agatgaaaga gacaaaaacg aaaggggagg ggaaaaaatt taataatatg atcgaaaggc
    14941 tttttacgtt tactaaagct caattttcag ctatgcttgg tggagcaatt gattacacta
    15001 cgatggtttt tttcactgaa gtaattcata ttcattatac tgtttccatt gttattggag
    15061 gaattgtagg agcattggtc aattttactg tcaataaagg ctggactttt cgttctaaag
    15121 aattacctta taaaagtact ggactaaaac aattactcaa gttttcgctg gtagttgcca
    15181 atagtatttt tttaaaggac gtaggtactt atttgattac tacttttgta aaaattgatt
    15241 ataaattcag taaaattatt gttgatttaa tagtttccat tggatttaat tataatttac
    15301 aaaaatattg ggtatttaaa aaagttccag taaacacaac taattaattc tgctatatga
    15361 caacaaagcc tttgaaatta atcaaaagct ttgttgtttt tactatattt aaaaatttct
    15421 tattatttta cttaatactg ctactactta agcctaactt tttctttacc aaaggtgtta
    15481 aatcattaga cttaggggat tgatagacaa tgacttgtgt gcttatgtca aatatataag
    15541 tataattatt ttctgcaccc acttcattga ttgctttttt aactttttca attacaggag
    15601 cgaaaagttc ttgttgcttt ttcataaggt cactatcagc cgtttgtcga aaggtagtaa
    15661 tacgttgttc catttcaaca atttcacttt ggcgagcctg ttttatactt tcaggcattg
    15721 tggcttgttt gtcttgaaat tctttaattt tagcataata ttcttgttgc attttcaata
    15781 gttcattttc ccattgattt tgcaaatcac ctaaagtttt ctcaatttgt gatctttcag
    15841 gcattaagga aagaatttct tgagaattaa tatgacctaa ttttaattct tgtccaatta
    15901 attttagagg aagaaggcag agtaacaaaa tagcaaattt cttcatgatg tctttgttta
    15961 aaaaattaat ataaaattag cgggtggcaa tatggcaagt ggcggattgc gggcttcatt
    16021 tttgacccac ggcactaaac ttgaagcgga ctacaaatgt ttgattacca acttgccccg
    16081 ccatttgcta tattgcgtgt tatgggctgg ttttctttgt caattagaat gatataccta
    16141 cattaatgct tattgcacct gagttttctg taaactcatc aatagaatac caatcatagt
    16201 agaaaaactt cattctttgc atttcatatc caagaccaac attcatagcg gacttttcag
    16261 aaactttaaa actgactccg attgttgggt taagaagaaa tccaactcct tcgaaactgt
    16321 tagtagcatc aaaagagtaa ccaacaccta atgataaata tggcgacaca ttattatcca
    16381 taaaattcgc ccggaaatca gcaaatattg gtatcaatgc tgcttctgca tcaaaatagt
    16441 aacgcaatcc tgttccaagg cccaatgaaa agtatggatt taattgataa ccatttatta
    16501 tgtttagttt caatctatcc atcccataat caccggtacc tatttggtag cccaattcaa
    16561 ctattccttt ataacctgat tgtaagcctg aattactaaa tgagccacca ctttttcctt
    16621 gaatcggttc ttttgtcaat ttttcgattt catccatttg atacacaaaa acacttctat
    16681 ctgctgtctc aattttaatt gatttatttg ggacttgttc aatgattacg ccccgaataa
    16741 tgcttccgtt tttaaggtaa acaacatctt gataattact ttgtccaaat gacatagtcg
    16801 tgattagtgc aaataaaagt aacgctaaat attttttcat cttaactcca ttttgttttc
    16861 ttactcgttt ggcttttcag ttccgccccg ttttttcaag tggtcgctgg tctccacatt
    16921 tttatacatt ttgtgtattt tgataattgt ccgaatatat caaaaatcag gtggttctct
    16981 tgtttgtttc attccgttgt gtttcttaaa cttgcccata ac
//
LOCUS       DAOSAS010000025        16743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_2368_length_16743_cov_9.835750, whole genome shotgun sequence.
ACCESSION   DAOSAS010000025 DAOSAS010000000
VERSION     DAOSAS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16743
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2368_length_16743_cov_9.835750"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..821)
                     /locus_tag="PLR72_02120"
     CDS             complement(126..821)
                     /locus_tag="PLR72_02120"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOK36040.1"
                     /translation="MKNIAVLTGAGMSAESGLSTFRDAGGLWEQHRVEDVATPEGFMQ
                     NPEMVLNFYNERRKQLLAAKPNAGHYTLAELEKDFEVHIITQNIDNLHERAGSSHVVH
                     LHGELMKVRSTGPGQEVYELSPDHLEIHVGDKCPKGYQLRPHVVWFGEPVPEIEKAIK
                     IVSEADIFVIIGTSMQVYPAAGLIHYVKPGTPIFLIDPKEVNITSSNVTVIQKGASEG
                     TKELLEILKKYKD"
     gene            complement(871..1947)
                     /locus_tag="PLR72_02125"
     CDS             complement(871..1947)
                     /locus_tag="PLR72_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOK36041.1"
                     /translation="MPEIKTEGIKYVGSKLKIIPCILQILSELKEVKKVLDGFSGTTR
                     VSQAFAQSGYNTTSNDIAVWSEVFATCYLKSKGSDTYYQQLLDYLNSLKGYDGWFTEH
                     YGADEASSKRPFQRKNTQKLDAIRNEIDRLNLNWEDKCVLLTSLIYALDAVDNTIGHY
                     AAYLSTWSRRSYHDLKLKLPARFKIISENTVSRKDIFETISEKSFDFAYFDPPYGSNN
                     EKMPPSRVRYAAYYHIWTTVILNDQPTLFGKVNRRLDSKDLLSGSVFEEFRKNKNGNY
                     LAMEALQKLIKNTQARYILLSYSSGGRATKKDLMDIVLSFGKLLKSIEIDYRKNVMSN
                     MCWTNEWAHHDNKHFEYLFLMEKF"
     gene            complement(2001..3872)
                     /locus_tag="PLR72_02130"
     CDS             complement(2001..3872)
                     /locus_tag="PLR72_02130"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOK36042.1"
                     /translation="MEEILEKDLLVDYGDSTIITLEGLEHVRRRPGMYIGKLGDGSHP
                     DDGIYVLLKEVLDNSIDEFRMGAGKTIEVQIQDGVVQVRDYGRGIPLGKMVEAVFIMN
                     TGGKYDSKAFKKSVGLNGVGMKAVNALSSTFIVQSFREGQSRRAEFSQGKLVRDSGIV
                     PDEGERGTLTIFVPDRTMFDGYVYKEEFVEAMLRNYAYLNTGLTLVFNGKKISSRNGL
                     SDLLNENLTSQPLYPLIHLIGEDIEIAFTHTNQYGEEYYSFVNGQHTTQGGTHQSAFR
                     EAVARTIKEFYSKNMELADVRNGIVAAIAISVEEPVFESQTKTKLGSRDMAPDGVSIN
                     KFISDFVKKELDNYLHRNPETADILFQKIQESEKERKAIAGITKIARERAKKVSLHNR
                     KLRDCRIHYNDTKGNTEESCVFITEGDSASGSITKSRDANTQAVFSLRGKPLNCYGLT
                     KKIVYENEEFNLLQAALNIEDGIDGLRYNKIILATDADVDGMHIRLLLITFFLQFFPE
                     LIKKGHLYILQTPLFRVRNKKETIYCYSEEEKKAAIARLGDQVEITRFKGLGEISPDE
                     FKNFIGEDMRLERVTLKKEDASAALLEFYMGKNTSERQNFIIENLKIEEENVEIEEM"
     gene            complement(4497..6812)
                     /locus_tag="PLR72_02135"
     CDS             complement(4497..6812)
                     /locus_tag="PLR72_02135"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK36043.1"
                     /translation="MISKKFVFVVICIVLITNYLTATNIQGKVMDENQQPIEFANVVL
                     YSLPDSTLVTGTITNKNGEFSLTANGTEKGFLKISFIGYETQTVPAISGQTIVLKVES
                     RLLNEVVVQKSLPKIQLKNDALVATVQNSVLSSAGTANDVLKRLPLLTGDDGVFSVFG
                     KGEAKIYINNREMRDVSELDNLNSADIKDVEIVTNPGARYDASVKAVIRIYTVRKAGD
                     GFGFDLRSSYNQSQNTDLTEQLNMNYRKDGWDVFGTFKFNRDAYFQDSKIWQETYVDT
                     LWRQENTLYSSGTSHTLTGIAGANYEFSQKHYAGIKYTYNAFPYNKWTSTMNSTVYAD
                     NVFYDKWSSVETDIAHNKPSHRVNSYYNGSFGNLNIDFNADFYTNKQHSSSETTETSQ
                     EYDNRKVNSENNVENRLIAEKLILTYPLLGGQFSLGNEYTNTHRTDEYLTEQNIVPSS
                     NTTIHEENNSFFAEYSRKISIANIGAGLRYENVNSEYFINDILSEEESRRYHQWFPNV
                     SCGAQIKNVNLQLSYTKKTERPTYRQLSSNVYYGNRFTLQTGNPFLKPTVVHNLTLVS
                     AWKFLQVMISYTNEKDAIIYWTEQLESNQAVSVIAYKNLEELPTFTASFNVSPTFGIW
                     SPQLNIGLLKQWLTVITNNQPITLNKLLTFVSLDNSFRLPQKYLLTLDGYFQTKGNYQ
                     NLYLARNRFIVNMGLTRSFFNDQLRIELKGQDLFYGMKDKNLLYNQKMQLLQTNHFDT
                     REIELTVRYNFNTAKSKYKGTGAGEKEMKRL"
     gene            complement(6978..7625)
                     /locus_tag="PLR72_02140"
     CDS             complement(6978..7625)
                     /locus_tag="PLR72_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HOK36044.1"
                     /translation="MEEKKNIELRSEKVRNIIGQIPSVWIRYGTLMIGLALLVLLLIS
                     AFIPYRETIPVRIQVKTIPQAEYVQADRNMFILINEWRDSVKTNSKEAFRGEIILNIK
                     GGDYVERGDTFFAVLPKQAQVYGTAEIAAASLAKIKEGDKVLIDDAVAGSLQGQVSEI
                     LPLPLSSNPSLRKIRIAFDEKTRWEKFIPESELQGKIVLSDVSLLTKFLQSIGLK"
     gene            complement(7631..9832)
                     /locus_tag="PLR72_02145"
     CDS             complement(7631..9832)
                     /locus_tag="PLR72_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOK36045.1"
                     /translation="MPFPFIQQPDAMDCGPACLCMVAEHYGKQYALEHLRNNCFIGRE
                     GVSMLGISKAAEKIGFHTVGGKLTFDKLAEKALLPCIVHWNQDHFVVVYNIKKNRKGY
                     EVYVADPGKGLIAYTQEEFCNHWISTQTGGEEKGVALLLEPTQLFYEQEGDQLRSKNR
                     VKFLGSYLLKYKRFFGQLILGLFIGSILQLIFPFLTQAIVDTGISGKDIHFIWLVLIA
                     EMMLLFSRTAIDFIRRKILLHISTRINVSLISDFFIKLMKLPMKFFDTKLTGDLLQRI
                     EDHRRIENFLTNQTISVIFSVFTFIIFSIVLFIYNVPIFLIFIVGSILYGAWIMIFLK
                     KRRLLDYKMFEQQGINRNVVYQLITGMQEIKLQGCEQRKRWEWEDVQADLFDVNMQSL
                     NLRQNQEAGGIFINELKNMLVTVLAATAVINGNLTLGMMLSIQYIIGQLNSPVEQIMG
                     FIYQWQDVSISLDHMNEIHTQQNEEDENRHIATLSDGNCGIRIQNLCFKYDGARPDYV
                     LNNINLTIPQNKVTAIVGASGSGKTTLIKLLLGFYNPNEGNIYVGKENLSEFSLSWWR
                     SQCGAVMQEGYLFSDTIARNIAVSDDEINLKRLRHAARVANIADYIEELPLGYNTKIG
                     QDGQGISQGQRQRILIARVVYKNPQIVLLDEATNSLDANNEKAIVENLSDFYKGKTVV
                     VVAHRLSTVKNADQIVVLDSGRIVETGSHNDLTKGKGKYYELVRNQLELGN"
     gene            complement(9843..12149)
                     /locus_tag="PLR72_02150"
     CDS             complement(9843..12149)
                     /locus_tag="PLR72_02150"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HOK36046.1"
                     /translation="MKHLYTSLLIIILAFAYYSDLCAQNIIKGKVVDENNIPIELVNV
                     ILYSLPDSSLITGSVTDSLGCFLISSDQIDNTFLRVSMIGYEPLNIKTKQVQMIMLQP
                     LATLLNEVTVSGRKKIFKMENGAIRVDIKNTVFETLTTGKEVIAQLPFVAVKDENFTI
                     LGKGKPLIYINNRLIRDENELNQLSPKDIKFIQIITTPGAQYDATVKSVIKITTEKTA
                     GEGISGMIYTKEGYSNVFFGNEYLSLNYRKESFDIFGSLNYVHDKYTSNFLSDQFISE
                     TKGRSEQITKENEDGKTIITNPMVGITFNPNKNISTGLRYTYNNNNYSGNAYNKIENI
                     QSSGTEQIAQNTYITRHPYTHQVNGYLDCSLSDKLSLNINMDYLKGDEKNIQDSYSVE
                     NPEDIIHNENLRNYSLFAVKGILSYSFTAGILSMGSEYSHTRIIQKYFINKPELGITN
                     TNDELKQNRSAIFATYQIQKGKYEMNVGVCGENICFNYFENEVLNKEQSKSYSKFFPN
                     LSLSYAEDNLQCMLGYERKIKYPTYSQLRSNVQYSSPFIYEAGNPYLLPEISSNITAM
                     FSWKDLQTMASYSFNRNDIQVVFSQFQDKSIILLKDENVPTSKEANIGISFAPTVGLW
                     KPRFEITGMKQWLHFENIPQNYSSPIFMAKWNNTFLFPADWTFRLDANYQSSGHSGVI
                     YLEPSWGVNLKLSKQFFNKKMNVMLAANDIFKTNRDKWNVNYDNIGMKYNRTIHSRLA
                     YITITYNFNSTKNKYKGQQASDEINRLK"
     gene            complement(12170..13636)
                     /locus_tag="PLR72_02155"
     CDS             complement(12170..13636)
                     /locus_tag="PLR72_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK36047.1"
                     /translation="MEKQYIINPRYTLRNESDKIVLLMKPDPVSPTSICVLHPIYAMI
                     LCFVDGCSIKEAAVKASNYLGIGEKEIMNIILPLIENKKIIKGYESIFPKDFLIEYKS
                     GMERRKYNPDNFYCSKLNLKLSRLNAPSDIICNITMKCYTNCAYCYADRHGHLNYKMS
                     PEKICNIIDEAYNLGVVSFKLIGGEIFQYKEWEVILRKLKSYGYNPCLSTKYPLKEKD
                     VQKLKEILDDKIPLQISLDTLIPEHIKNILHVRGEKYKDDIIYFMELLEKYEVNYTIH
                     TVLNLYNDSLEDIKSIENHLINKKYLQEWSVDSAKCSMYLPYDYSSYKPSKENVIKIA
                     SYLKNLSNSISSFKIHLPEILRNHNKIDRNKKSKIFKNRISCSGNNSSMYILPDGKVT
                     ICEELYWHPHFIIGDLNLNSIEEIWNSPKAHSLFYLNQSDISKDSPCSTCKDFKDCHS
                     YKHVCWRDVILAYGSNNWDFPDPFCPLAPPILKDIAME"
     gene            complement(13706..13885)
                     /locus_tag="PLR72_02160"
     CDS             complement(13706..13885)
                     /locus_tag="PLR72_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36048.1"
                     /translation="MKKNIKNEIFLKDLEIIKEFQLEEIKGGADAQLAKACAACCLIL
                     GGGGSAPIPPIEVAI"
     gene            complement(14169..14318)
                     /locus_tag="PLR72_02165"
     CDS             complement(14169..14318)
                     /locus_tag="PLR72_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36049.1"
                     /translation="MKKILFLKEDDLLSFTHLSRINGGSNNIYENEMVIIYINGKPVI
                     IKKEQ"
     gene            14555..15946
                     /locus_tag="PLR72_02170"
     CDS             14555..15946
                     /locus_tag="PLR72_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOK36050.1"
                     /translation="MKRNVLILFSAVAIVLLIVSQIIWIQQLMAREKQRLEMELKQTL
                     QSIVAFCLSREVGNKTTQMLSLELIPIDPDKIPKNAVIKGSFDTKEYSSDKNLGNYLV
                     GVFAEDLLQENKIPLEPIDSLFRKEFAFYPEISEYSMSIEKNDSILRKLYVGENTCSV
                     LKDANSGVNIRIPIGKTGKYWYHAQVIFKPTIFTQRLQLMTALSAIAVIVISVLLLYQ
                     LALLRQQTRELAIHKHVAMGIVHDLKSPLAYVYTLLGFFEKSENEVQKKGMFYSAKVR
                     VKYLSEKIEILLSVMKSKEHFFQIHCKPYDFNLRCKEIMEELCVIYKGKDIAWCLEPA
                     EGTILSVDPVYFDGCVRNLLDNAVKYSGDKPFIKILTIDHNKHIFLSFADNGKGILKK
                     ERKKIFREFYRHDKSSSVKNHGIGLAFTRQVVRAHSGKIFVDSKEGEGSIFTIVFPKK
                     IAEKYGTNKSNIG"
     gene            15918..16592
                     /locus_tag="PLR72_02175"
     CDS             15918..16592
                     /locus_tag="PLR72_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOK36051.1"
                     /translation="MEQIKVILVDDDTDLGNLISLSLSSDGYKVHYQNSLAGIEAIIQ
                     EFEPSILVLDVEVGEDDGIEKAKEIILTHPSLPILFISSHTDIESINRGISTGGVHYL
                     RKPFEIQELEIYINRFALSENHKEIIAAIGSYSLNFDSRELFYKHTYIKQLSPLEYSV
                     LILLFQNRNEVIANDTLSEKVWSKKFPDTEASINNVISKLRKLLKNDPNVCIQTIKNR
                     GYKLIF"
BASE COUNT         5103 a         3101 c         2763 g         5776 t
ORIGIN      
        1 tcttcctttt tcatccgtaa ataattttat taagtcataa attcaagtaa ttaatgcaac
       61 tttttcagag agaatgaaag actaaacttc aacaaaagaa aattattgaa aaatttaatc
      121 ccttttcagt ctttgtattt tttcaggatt tccaacaatt cttttgttcc ttcgctggct
      181 cctttttgaa taacagttac attagacgaa gtaatgttta cctctttggg atcaattaaa
      241 aaaatgggcg tgcctggttt tacataatga attagacctg ctgctggata aacctgcatc
      301 gaagtaccaa tgataacaaa aatatcagct tcggaaacaa ttttgatggc tttttcaatt
      361 tccggtacag gctcgccaaa ccataccacg tgcggtcgca attgataacc ttttgggcat
      421 ttatcaccaa catgaatttc gagatgatca ggactcaatt cataaacttc ctgaccaggt
      481 ccagtagatc ttactttcat cagttcacca tgtaaatgaa ccacatggct acttccagct
      541 ctctcatgga gattatcaat attctgtgtg ataatatgta cctcaaaatc tttttccaac
      601 tctgccaatg tataatgtcc tgcatttggc ttggcagcca gtaactgttt gcgacgttca
      661 ttgtaaaaat ttagtaccat ttcaggattt tgcataaatc cttcgggtgt ggctacatcc
      721 tctacccgat gttgttccca gagcccgcca gcatcgcgaa aagtggatag tccactttcg
      781 gcactcatgc ctgctcctgt taaaactgca atgtttttca ttttttatat gttattaatt
      841 tttaaaaatc agatagttaa ataaattagt ttaaaatttt tccattaaaa acaaatattc
      901 aaaatgttta ttatcatgat gagcccattc gttggtccaa cacatattac tcattacatt
      961 ttttctataa tcaatttcaa tggattttag caatttccca aaagaaagta caatatccat
     1021 caaatctttt ttggttgctc tgccgccaga actgtaagaa agaagaatat aacgagcctg
     1081 cgtatttttg attaactttt gcaatgcttc cattgcaaga taatttccat ttttattttt
     1141 tctgaattct tcaaaaacag agcctgataa taaatctttg ctatctaatc gcctgttgac
     1201 ttttccaaaa agcgttggct gatcgttaag aattacagtt gtccagatat gataataggc
     1261 agcatacctc actctgctgg gcggcatttt ttcattattg gaaccataag gcggatcaaa
     1321 ataagcaaaa tcgaaagatt tttcggaaat ggtttcaaaa atatcttttc gggaaacagt
     1381 attttcagaa atgattttaa atcgtgcagg taattttaat tttaaatcat gatatgagcg
     1441 ccgcgaccat gtagaaagat aagctgcata atgtccaata gtattatcaa cggcatcaag
     1501 tgcataaata agactggtca gtagaacaca tttatcttcc caattcaaat ttaatctgtc
     1561 aatttcattg cgaatagcgt ctaatttttg ggtatttttt cgctgaaaag gtcgtttgct
     1621 gctggcttca tcagctccat agtgttctgt aaaccagcca tcataacctt tcaaactgtt
     1681 taaataatcc aggagttgct ggtaataagt atcactacct tttgatttga gatagcaagt
     1741 agcaaaaact tctgaccaaa ctgcaatatc attggaagta gtattgtaac ctgactgagc
     1801 gaatgcctga gaaactctcg tagtaccgct aaaaccgtcg agtacctttt ttacctcttt
     1861 taattcagaa agaatctgca aaatgcaagg aatgattttt aattttgaac caacatattt
     1921 tattccttct gttttgattt caggcattta tttatcgcac aaattaaata agaaaatttt
     1981 ctacaaaaag gagaagagaa ttacatttct tctatttcca cattttcctc ctcaattttc
     2041 aggttttcaa tgataaagtt ttgacgttca gaagtgtttt tccccatata aaattccaat
     2101 aatgcagcag aagcatcttc ctttttaagt gttacccgtt ccagccgcat atcttctcca
     2161 atgaaatttt taaattcatc aggagaaatt tcacctaatc ctttgaatcg cgttatttct
     2221 acttgatcac ccaaacgagc aatagcagct tttttttctt cttctgaata acaataaatt
     2281 gtttcttttt tatttcttac tctgaaaagt ggtgtttgaa gaatatataa atgtcctttt
     2341 ttgatgagtt caggaaaaaa ttgaaggaaa aaagtaatca acagcaggcg aatatgcatt
     2401 ccatctacat ccgcatcagt agcaagaata attttattgt aacgcagtcc atcaatgcca
     2461 tcttcaatat ttaaagccgc ttgcaataga ttaaattctt cattttcata aacaattttt
     2521 ttggtaagtc cataacagtt taatggcttg cctctcaagc tgaaaacagc ctgcgtattg
     2581 gcatctctgc ttttggtaat agaaccgctt gccgaatcgc cttccgtaat aaaaacacat
     2641 gattcttccg tatttccttt tgtatcgtta taatgaatcc ggcaatcgcg taattttcgg
     2701 ttatgcaagc tgactttttt tgcccgttca cgtgctattt tggttatgcc ggcaatggct
     2761 ttcctttctt tttctgattc ttgaattttc tggaataaaa tatctgccgt ttcaggatta
     2821 cggtgcaaat aattatccag ttccttttta acgaaatcag aaataaattt attgatagaa
     2881 acaccatctg gtgccatgtc ccgagagcca agcttggttt ttgtttgcga ttcgaataca
     2941 ggttcttcaa cgcttatggc tatagctgct acaatgccgt ttcgaacatc agccaactcc
     3001 atatttttag aataaaattc cttgatggta cgtgccactg cttcacgaaa agcactttga
     3061 tgagttcctc cctgtgtggt atgctgacca tttacaaagg aatagtattc ttcaccatat
     3121 tgattggtat gtgtaaaagc aatttcaata tcttcgccta ttaaatgaat gagaggataa
     3181 aggggttgag acgtcagatt ttcattcagt aaatcactca aaccattacg ggaagaaatt
     3241 tttttcccgt taaaaacaag agtgagccca gtattcagat aagcatagtt tcgcaacatg
     3301 gcttctacaa attcttcctt gtaaacatag ccatcgaaca ttgttcgatc gggaacaaaa
     3361 atcgtaagtg tcccacgttc gccttcgtca ggaacaattc cgctatccct taccagtttc
     3421 ccttgactga attctgcccg acgagactga ccttctctaa agctttgaac aataaaagta
     3481 gaagaaagtg cgttaacagc tttcatgccg acaccattca atccaaccga ttttttaaaa
     3541 gcttttgaat catattttcc acctgtattc attataaaaa cagcttctac catttttccc
     3601 aaaggaattc cacgtccata atcgcgtact tgcactacac cgtcttgaat ttggacttca
     3661 atggttttac cagcgcccat tctgaattca tcaatactgt tgtccaaaac ctctttcagc
     3721 agaacataaa ttccatcatc aggatgcgaa ccatcaccca gttttccaat atacattcct
     3781 ggacgacgac gaacatgttc caagccttcc agcgtgataa tggtgctatc gccgtaatca
     3841 accaatagat ctttttctaa aatttcttcc atattgatta aaaaatcaaa aacaatctct
     3901 tgaattttaa aaattttctt taaaaattat tttattgaag ctgattttta aatttaagat
     3961 gatagtaatt cttgtttcag agcaaatcta actctgatta tttgtacaaa aataagaaat
     4021 ttctgggata ttttaaaaaa atattgcttc taaagctctt ttgctgattt ttttatcaaa
     4081 cgatttaatt ataagatttt gatttttttc ttcaaaattc tttacttaga aactcgtaac
     4141 ttattttacc tcttgcaccc tgaactatgt ttttttatta tcaatgcctg aaataagttg
     4201 attactttga aataatatga cgttactttt acaaataaac aattgaatac tttttaaaaa
     4261 aggggcttcc ccaacccccc tacttactcc tttgggaaat gtttaatttt tttaattatt
     4321 tcggcgattt tactttactt taattgtatt aacaagatca aaatttctct acaaaaaatc
     4381 ttcttttctc tcgcaaactc ataccttgta ctttgaatct cgcaactctc caaactccaa
     4441 acttcagatt ttcaactatt cttgaatcat ttttttattt tataagctac ttgggatcaa
     4501 agtcgtttca tttctttctc gccagcccca gttcctttgt atttgctttt tgcagtattg
     4561 aaattgtagc ggacagttaa ctcaatttcg cgggtatcaa aatgattcgt ttgtaataac
     4621 tgcatttttt gattataaag taaatttttg tctttcatcc cataaaacaa atcctgtcct
     4681 tttaactcta tacgaagctg gtcgttgaaa aaagagcggg ttagccccat gttaacaata
     4741 aatctgtttc ttgccaaata taaattttga taattccctt tcgtttgaaa atagccatcc
     4801 agtgttagta aatatttttg aggcaaacga aaagagttgt caagcgaaac aaaagttaag
     4861 agtttattta aagtgattgg ctgattgttg gtaattaccg taagccattg ttttagcagt
     4921 ccaatattta attgtggtga ccagatacca aaagttggag atacattaaa ggaagctgta
     4981 aaagtgggta attcttccaa attcttgtat gcaatgacag aaacggcttg atttgattcc
     5041 aactgttctg tccagtaaat aatagcatcc ttttcgttgg tataactaat catcacttgc
     5101 aaaaatttcc atgcacttac caaagttaag ttgtgaacta ccgttggttt caggaaagga
     5161 ttgccggttt gcagggtaaa acgatttcca taatatacat tgctgctcaa ttgacgataa
     5221 gtcgggcgtt ctgttttttt cgtgtagctc aattgtaaat ttacattttt aatttgcgcc
     5281 ccacatgaaa catttggaaa ccactgatga taacgtcggc tttcttcttc gctaagaatg
     5341 tcgttgatga aatattcgga attgacgttt tcgtaacgca atcctgctcc aatattggca
     5401 attgatattt ttcgggaata ttccgcaaaa aaactgttgt tttcttcgtg tatggtcgta
     5461 ttggaagaag gaacgatatt ctgttccgtc aaatattcgt cggttcgatg cgtattggta
     5521 tattcgttgc ccaatgagaa ctgcccgccc agcagcggat aagttaaaat cagcttttcg
     5581 gcaattaatc ggttttctac attgttttca gagttaacct ttcgattgtc gtattcttga
     5641 ctcgtttcgg ttgtttcaga tgacgaatgt tgcttgtttg tataaaaatc agcattaaaa
     5701 tcgatattca aattgccaaa acttccattg taataactgt tgacgcgatg ggacggcttg
     5761 ttgtgagcaa tatcggtttc tacgctgctc catttgtcat agaaaacatt gtctgcatat
     5821 acggtgctgt tcattgtgga agtccattta ttgtatggaa aagcattgta cgtatatttt
     5881 attccggcat agtgcttttg agaaaattcg tagtttgcac ctgcaatacc ggtcaaagta
     5941 tgggaagttc cgcttgaata aagtgtattt tcctgtctcc agagcgtatc aacataggtt
     6001 tcttgccata ttttactgtc ctgaaaatag gcatcccgat tgaacttaaa tgttccaaac
     6061 acatcccaac catcctttcg ataattcatg ttcaattgct ctgtcaaatc ggtattttgt
     6121 gattgattgt agctggagcg caagtcaaag ccaaaaccgt cgccggcttt acgtacggta
     6181 taaatgcgga taactgcctt caccgaagca tcgtagcgag caccgggatt ggtaacaatt
     6241 tccacgtctt tgatatctgc cgaattcagg ttgtccaatt cggaaacatc acgcatctca
     6301 cggttgttga tatagatttt tgcttccccc tttccaaaaa ccgaaaaaac accatcgtcc
     6361 cctgtaagta gtggaagtcg tttgagcaca tcgtttgctg tgccagcgct actgagtacc
     6421 gaattttgca ctgtcgccac taacgcatca tttttgagtt gaattttggg caaacttttt
     6481 tgcaccacca cttcgtttaa aagccgcgat tctactttta atacaatggt ttgacctgat
     6541 atagcaggta cggtttgagt ttcgtagcca ataaaagaaa ttttgagaaa acctttttca
     6601 gtgccattgg ctgtgaggga aaattctccg tttttgttgg tgatggttcc ggtaacaagc
     6661 gtgctgtcgg gcagtgaata caagacaaca ttggcaaatt cgatgggttg ctgattttca
     6721 tccattactt ttccttgaat gttggttgca gtcaaataat tggtaatcag aactatgcag
     6781 attacgacaa aaacgaattt ctttgagatc atgaagattt ctctattttg ttaaaataat
     6841 attttacaaa agtaaaataa attattgaac ctgtttttaa ctactatcca atatttgctt
     6901 aatcaatctt aaccaaaaaa tatctatttt agaattatat ctttttccaa agggaaaata
     6961 atgccagtaa aataaaatta tttcaaccct attgattgta agaatttggt caacaacgac
     7021 acatccgaca aaacgatttt tccttgcaat tcactttcgg ggataaattt ttcccatcgc
     7081 gttttttcat caaaggcgat acgtattttt ctcaaggatg gattggatga aagaggcagc
     7141 ggaagaatct ccgatacttg gccttgcagg cttcccgcaa cagcatcatc gatcaaaacc
     7201 ttatctcctt cttttatttt cgccaacgaa gcggcagcaa tttcggctgt tccgtaaacc
     7261 tgcgcctgtt tcggcaaaac ggcaaaaaat gtatcacccc tttctacata atcaccgccc
     7321 tttatattca gaatgatttc tcctctgaaa gcttcttttg aatttgtttt gacggaatca
     7381 cgccattcgt ttatcaatat aaacatattt ctgtccgcct gaacatattc ggcttgcgga
     7441 atcgttttca cttggatgcg aacagggatc gtttcgcgat aaggaatgaa agccgaaatc
     7501 aataacagaa ccaacagcgc caaccctatc attagcgtac cgtaacgaat ccatacggaa
     7561 ggaatctgtc cgatgatgtt cctcactttc tcgctgcgaa gctcgatatt tttcttttct
     7621 tccattccca tcaatttcca agttcgagtt gatttcttac caattcgtaa tatttgcctt
     7681 ttccctttgt caagtcgtta tgtgaaccgg tttcgacaat tcttccgctg tcgagaacaa
     7741 caatttgatc ggcattcttt accgtgctca atcgatgagc caccacgaca accgtcttcc
     7801 ctttataaaa atcggaaaga ttttctacaa tggctttttc gttgttggca tccaacgaat
     7861 tagttgcttc gtcgagaagg acaatttgcg gattcttata cactacccgg gcaataagaa
     7921 tgcgttgccg ttgcccctga ctgataccct gtccatcttg accgatcttc gtgttgtatc
     7981 ccaacggaag ctcctcgatg tagtccgcaa tattggctac acgggcagcg tggcgcaacc
     8041 gtttcagatt aatttcatcg tccgatacgg caatattgcg ggcaatcgtg tccgaaaaca
     8101 gatacccttc ctgcattacc gctccacact gactgcgcca ccacgacaag ctgaactctg
     8161 ataaattttc cttccccaca taaatattcc cttcgttggg attataaaaa ccgagtaaca
     8221 attttatcaa tgtggttttt ccgctgccgc tggctcccac aattgccgtt accttgtttt
     8281 gtgggattgt caggttgatg ttgttcagca cgtagtccgg acgtgctcca tcgtacttga
     8341 agcaaagatt ttggattcgt attccgcaat ttccgtccga aagcgtcgct atatggcggt
     8401 tttcgtcttc ttcgttctgt tgggtatgaa tttcgttcat gtggtcgaga ctgatgctca
     8461 catcctgcca ttgatagata aaacccataa tctgttccac cgggctgttc aattgcccga
     8521 tgatgtattg aatggatagc atcataccca aagtcagatt gccgttgatt actgccgtag
     8581 cggcaagtac ggttaccaac atatttttca gctcgttgat aaagatgcca cctgcttcct
     8641 gattttgccg aaggttgagt gattgcatgt ttacgtcgaa cagatcagcc tgcacatctt
     8701 cccattccca gcgtttacgt tgttcgcagc cttgcagttt gatttcctgc atgccggtaa
     8761 tcagttgata tactacattg cggttgatgc cttgctgctc gaacatttta taatccaaca
     8821 gacgcctttt tttcaaaaag atcatgatcc atgcaccata gagaatactg ccgacaatga
     8881 atatgaggaa gatgggtacg ttgtagataa acaggacaat ggagaagatg ataaaagtaa
     8941 atacggaaaa aataacggaa atcgtttgat tggtcaggaa attttcaatg cggcgatggt
     9001 cttcgatacg ttgcagcaaa tcgcccgtga gtttggtatc gaaaaatttc atcggcagtt
     9061 tcatcagctt gatgaaaaaa tcggaaatga gcgaaacgtt gatgcgggtg ctgatgtgca
     9121 gcaaaatttt gcgccggatg aaatcgatag ccgtacgact gaacaacagc atcatctcgg
     9181 ctatgagtac cagccagata aaatgaatat ctttcccgct gatgccggtg tccacgatgg
     9241 cctgcgtgag aaacgggaag atgagctgaa ggatgctgcc gatgaacaat cccaatataa
     9301 gctgcccgaa aaaacgcttg tatttcaaca gataactacc gagaaatttt accctgtttt
     9361 tcgaccgcag ttgatcgcct tcctgttcat agaacaattg ggtcggttcc aaaagcaaag
     9421 ccaccccttt ttcttcgcca ccggtttggg tgctgatcca atgattgcaa aactcttcct
     9481 gcgtataggc aatcaatcct tttccgggat cggcaacata tacttcatat cctttcctat
     9541 ttttcttgat attgtaaacc actacgaagt gatcctgatt ccaatgcacg atgcacggca
     9601 gcaatgcttt ttctgccaat ttatcaaacg tgagctttcc gccgaccgta tgaaagccga
     9661 ttttctccgc cgctttgcta attcccagca tcgacacgcc ttcccgccca ataaaacagt
     9721 tgttacgcaa atgttccaat gcgtattgtt ttccataatg ctctgcaacc atgcagagac
     9781 atgccggacc gcagtccatc gcgtcgggtt gttggatgaa agggaagggc attattaaat
     9841 ttttatttta atcggtttat ttcgtcgctt gcctgttgac ctttgtattt atttttcgtt
     9901 gaattgaaat tgtatgttat tgtaatataa gcaagacgtg aatgaattgt tctattgtat
     9961 ttcataccga tattatcata atttacattc catttatcgc gatttgtctt aaatatatca
    10021 tttgcagcaa gcattacatt cattttctta ttaaaaaact gtttggacaa tttaagattg
    10081 acaccccatg atggttctaa atatataact ccactatgtc cgcttgactg ataatttgca
    10141 tctaaacgaa aagtccaatc tgcaggaaat aagaaggtat tattccattt tgccataaaa
    10201 atgggagagg aataattttg aggaatattt tcaaaatgca accattgttt catccccgta
    10261 atttcaaatc tcggtttcca tagtcccaca gtgggagcaa acgaaatacc aatattggct
    10321 tctttacttg taggcacatt ttcgtctttc aataaaataa tcgatttatc ctgaaactgt
    10381 gaaaaaacca cctgtatatc atttctatta aatgaatagc ttgccattgt ctgcaaatct
    10441 ttccatgaaa acatcgctgt aatattactg gaaatctctg gcaataaata tggattacct
    10501 gcttcataaa tgaaaggtga tgaatattgc acattgctcc tcaactgtga ataagtcgga
    10561 tattttattt tacgctcata tccaagcata cattgaagat tgtcttctgc ataagaaagc
    10621 gaaaggtttg gaaaaaattt gctgtaactt tttgattgtt ctttgttcaa cacttcattt
    10681 tcgaaataat taaaacaaat attttcgcca catacaccca cattcatttc atactttcct
    10741 ttttgtattt gataggtagc aaatatggca cttcggtttt gttttaattc atcattggta
    10801 tttgttatgc ccaattcagg tttattgatg aaatattttt gaataatacg ggtatgagaa
    10861 tattctgaac ccatggataa aattccggcc gtaaagctgt aagacaatat tcctttgact
    10921 gcaaataaag aatagttacg aagattttca ttgtggatga tatcttccgg attttccaca
    10981 ctgtaggaat cttgtatgtt tttttcgtca ccttttaaat agtccatatt tatattaagc
    11041 gataacttat cgcttaaaga acaatctaaa taaccattaa cttgatgtgt gtaaggatga
    11101 cgtgttatat aggtgttttg agctatttgt tctgtcccgg atgattgaat attttcaatt
    11161 ttattatatg cattaccgct ataattgtta ttattatacg tatatctcaa gccggttgat
    11221 atatttttat tggggttgaa ggtgattccc accataggat tggttattat tgtttttcca
    11281 tcttcatttt ctttagtaat ttgttcactt ctgccctttg tttcacttat aaattgatca
    11341 gataaaaaat tagaggtata tttatcatgt acataattta atgagccaaa aatatcaaaa
    11401 ctttcttttc gataattgag tgaaaggtat tcgttcccga aaaaaacatt tgaatatcct
    11461 tctttagtat aaatcatgcc gcttattcct tctccagcag ttttttccgt agtaatttta
    11521 atcactgatt ttacggtagc atcatattga gctcccggag tggttattat ttgaataaat
    11581 ttaatgtcct tcggcgaaag ctgattcaat tcattttcat ctcttattaa tctgttatta
    11641 atatatataa gtggtttacc cttacctaat atggtaaaat tttcatcttt aactgcaacg
    11701 aaaggtaatt gcgcaattac ttctttacct gtggtaagtg tttcaaaaac agtatttttt
    11761 atgtcaactc taatcgcacc gttttccatt ttaaaaattt tttttctccc agaaacagtt
    11821 acttcattta acaacgtggc taatggttgc aacataatca tctgaacctg tttcgtttta
    11881 atgtttagag gctcatagcc gatcattgaa acccgtaaaa atgtgttgtc aatttggtct
    11941 gaagagatca agaaacaacc taaactatcc gtgaccgatc cagtaatcag tgaagaatcg
    12001 ggcaacgaat ataatattac gttgaccaat tcaattggaa tattattctc atcgaccacc
    12061 ttccctttta ttatattttg agcacataaa tctgaataat aagcaaatgc taatattatt
    12121 attaatagag aagtgtataa atgtttcatt gtttttatat attttggaat tattccatag
    12181 ctatatcttt taaaatcgga ggagccagag ggcaaaatgg atccggaaaa tcccaattat
    12241 tactaccgta agctaaaatt acatctctcc aacaaacatg tttataggaa tggcaatctt
    12301 tgaaatcctt acatgtacta caaggactat cttttgatat atctgattga tttaaataaa
    12361 agagtgaatg agcttttggt gaattccata tctcttcgat actatttaaa tttaaatccc
    12421 ctataataaa atgaggatgc caatataatt cttcacaaat tgttactttt ccatctggta
    12481 aaatatacat tgaactatta tttcctgaac aagaaatacg gtttttgaaa attttacttt
    12541 ttttgtttct atctatttta ttatgatttc tcaaaatttc gggaagatga attttaaaag
    12601 aactaatact atttgataaa tttttaagat atgaagctat ttttataaca ttctcctttg
    12661 aaggtttata agaagaataa tcataaggta aatacatgga acattttgct gaatcgacag
    12721 accattcttg aagatatttt ttattaatta aatgattttc aatagattta atatcttcca
    12781 gactatcatt atataaattt aatactgtat gaattgtata gttaacttca tatttttcta
    12841 aaagttccat aaaataaata atgtcatctt tatatttttc tccccttaca tgcaaaatgt
    12901 ttttgatgtg ttcagggata agggtatcta aagaaatttg taaaggaatt ttatcatcta
    12961 aaatttcttt aagcttctga acatcttttt cctttaatgg atattttgtg gaaaggcaag
    13021 gattataacc ataagatttt aatttcctca atataacctc ccattcttta tactgaaaaa
    13081 tctctccacc tattagtttg aaacttacta cccccaaatt atatgcttca tcaataatat
    13141 tacaaatttt ttcaggagac attttataat ttaaatgtcc atgtctatca gcataacaat
    13201 atgcacaatt tgtataacac ttcatcgtga tattacaaat gatatcagaa ggagcattta
    13261 atcttgataa ttttaaattt aatttagaac aataaaaatt atcaggatta tatttacgtc
    13321 tttccatccc tgacttatat tcaatcaaaa agtctttagg aaatatcgat tcatatcctt
    13381 ttattatttt tttattttca ataagaggaa gtataatatt catgatttct ttttcaccaa
    13441 tccctaaata gtttgaagct tttactgcag cttcctttat agaacatcca tcaacaaaac
    13501 ataaaatcat tgcataaata ggatgcaaaa cgcaaatcga tgttggagat actggatcag
    13561 gtttcattaa aaggacaatt ttgtccgatt catttctcag agtatatcta ggattaataa
    13621 tatattgttt ttccataaaa agatatttaa aagtaacgga taatatatcc gttactcaca
    13681 attaaaaata gttctatttt tatctttaaa ttgctacttc tataggtggt ataggagcac
    13741 tacctccacc accaagtatt aaacaacaag cagcacatgc tttagcaagt tgtgcatctg
    13801 cgcctccttt aatttcttca agttgaaatt ctttaattat ttctaagtct tttaaaaaga
    13861 tctcgttttt aatatttttt ttcataatca taattattaa aacatttttc tactcgtttc
    13921 gcttttcgaa atccgcgtcc attgtttcat acagcaaccc gagaattgat ttcgggggaa
    13981 attactgcac catcctactg gctacttttt tcactttcag tggctactgg taaaaaaatc
    14041 accatgtttt tttattatct ttgcaatgcc ttttcttgtg ggatttgtga tacatctggt
    14101 ttgttacctc aaattttatt gttcgctttc ctggcaaggg aaggcttttt tgtaattatt
    14161 aagtttcgtc attgttcttt tttaattatg acgggtttac catttatgta aattattacc
    14221 atttcatttt cgtaaatatt gttacttcct ccatttattc gtgacaaatg agtaaaagac
    14281 aataaatcgt cttcttttaa gaataaaatt tttttcataa tcgataatat ttatttaagg
    14341 tttattttac aaaagtaaaa tgagttattg aatctgtttc taatcaacgt ctaacatttg
    14401 tctaaccaat ctaaccaaaa taattaaatt aacaatcttt aactaatcga tttttttatt
    14461 cccaaacaag tggaaaaatt caattaattc atctgcatgt aaatcttttt tgttttttac
    14521 ttacatttgc acttacatta caaaatacta atacatgaaa cggaatgtac tgattctatt
    14581 ttccgctgta gccattgtac tactgatcgt ttcgcagatt atatggatac aacaattgat
    14641 ggcacgcgag aaacaacgtc tcgaaatgga attgaagcaa acccttcaaa gtattgtggc
    14701 attttgttta tcgcgagaag ttgggaataa aaccacacaa atgttaagtt tggaattgat
    14761 tccaatagat ccggataaaa ttccaaaaaa tgctgtcatc aaaggttcgt ttgatacaaa
    14821 agaatattct tccgataaaa atttgggaaa ttatttggtt ggcgtttttg ccgaagatct
    14881 tttgcaggaa aacaaaattc cgctcgagcc cattgactca cttttcagaa aagaatttgc
    14941 cttttatcca gagatttctg aatattccat gtctatcgaa aaaaatgatt cgatattgcg
    15001 taagttatac gtaggagaaa atacatgttc tgtcttgaag gatgcaaaca gcggagtaaa
    15061 cataagaatt cccataggaa aaacaggtaa gtactggtat catgctcagg taatattcaa
    15121 accaactatt ttcacacaac ggcttcagtt gatgactgct ctttcagcta tagcggtgat
    15181 tgtaatttca gtgttgttgc tttatcaatt ggctttgttg agacaacaaa cacgggaact
    15241 ggcaatacat aaacatgtag caatgggtat tgtccacgac ctgaaatcgc cacttgctta
    15301 tgtctatacc ttgctgggct tttttgaaaa aagtgaaaat gaagttcaaa agaaaggaat
    15361 gttttactcg gctaaggtgc gtgtaaaata tttatcagaa aaaattgaga ttcttttatc
    15421 agtgatgaag agtaaggaac atttctttca gatacattgc aaaccttatg attttaactt
    15481 gcgttgcaag gaaataatgg aagaactctg tgtaatttac aaggggaaag atattgcatg
    15541 gtgtttagaa cctgctgaag gaacaatact ttcggtagat cctgtttact tcgacggctg
    15601 tgtaagaaat ttattggaca atgccgtgaa atattctggc gacaaaccat tcataaagat
    15661 tttgactatt gatcacaata aacacatttt tctttctttt gccgacaatg ggaaaggtat
    15721 tttaaaaaag gaacgaaaga aaatatttcg tgaattttac cgtcacgata aaagttcatc
    15781 ggtaaaaaat catggtatag gacttgcttt taccagacaa gttgttcgag cacattccgg
    15841 aaaaattttc gtcgatagta aagaaggaga aggaagtatt tttactattg ttttccccaa
    15901 aaaaattgct gaaaaatatg gaacaaataa aagtaatatt ggttgacgat gataccgatt
    15961 tgggcaattt aataagttta tctctttctt ctgatggtta taaagtgcac taccagaatt
    16021 ctttggcagg aattgaagcc attattcagg aatttgaacc ttccatttta gtattggatg
    16081 ttgaggtagg tgaggatgat ggtatagaaa aggcaaaaga aattatcttg actcatccct
    16141 cgttgccaat tttgtttatt tcctcacata ccgacataga aagtattaac agaggtattt
    16201 ctactggtgg tgttcattat cttcgaaaac ctttcgagat acaggaactg gaaatctata
    16261 taaatcgttt tgctctttct gaaaatcata aagaaattat tgcagcaatt ggcagttatt
    16321 ctttgaattt tgatagccga gaactttttt acaaacatac ttatatcaaa cagcttagtc
    16381 ctctggaata tagcgtatta atactattgt ttcaaaatag aaatgaagtg attgctaatg
    16441 atactctttc tgaaaaagta tggagtaaaa agtttcctga tacagaagcg tcaataaaca
    16501 atgtgatatc gaaattaaga aaattattga aaaatgatcc taatgtgtgt atccaaacaa
    16561 taaaaaacag aggatataaa ttaatttttt gatttacaat agttttataa ttttagtgaa
    16621 aaataattaa gttatctgaa tgataataat ttcctgaatt tttatactaa ctgtactttt
    16681 ccctaccgtt ttaaaatatt tttttgtctt taacttttat tttgaaaata caagctacaa
    16741 att
//
LOCUS       DAOSAS010000026        16479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_2416_length_16479_cov_12.101559, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000026 DAOSAS010000000
VERSION     DAOSAS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16479
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2416_length_16479_cov_12.101559"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLR72_02180"
     CDS             complement(<1..335)
                     /locus_tag="PLR72_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOK36052.1"
                     /translation="MLFKLESPYRPTGDQPEAIQQLVEGIKANVPYQTLLGVTGSGKT
                     FTIANVIEQVQKPTLVLSHNKTLAAQLYSEFKSFFPHNAVEYFVSYYDYYQPEAYLPL
                     TDTYIEKDLS"
     gene            complement(461..679)
                     /locus_tag="PLR72_02185"
     CDS             complement(461..679)
                     /locus_tag="PLR72_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOK36053.1"
                     /translation="MFMSKRLTRSNKNKLLAGVCGGIAKFLGWDPTIVRILYVLLSIL
                     SAAFPGILVYLILWLVMPLEKETEEKTE"
     gene            complement(908..1741)
                     /locus_tag="PLR72_02190"
     CDS             complement(908..1741)
                     /locus_tag="PLR72_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36054.1"
                     /translation="MTYKKVSLNLIIVSIIFFFISCNSRSKQEKEADQHLQEIEQLIQ
                     QNELNAAKITIDSFHAKYPRLVAKRRIAEAYKDTITRRECRKTIAYCDSLLPIKEREA
                     DSLLQYFRLEKNPKYQEIGNYVYKTQSTEANANRTYLKAYVDENADYYLISQYVGPKI
                     EHNSVEISNGEVFAHTDTIDISSPFYLSFSDETGRWEIVTFKNEAENGVSAFVSQYIN
                     EPLKVTLHGRKNYSYFLQNADKKAIMETYHLWVVKKDINRLKKEIQKAQLKIEQINKR
                     N"
     gene            2015..2791
                     /locus_tag="PLR72_02195"
     CDS             2015..2791
                     /locus_tag="PLR72_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOK36055.1"
                     /translation="MQKFWYLTVFMLWIIFQFTSCITARKVNYLQEPSQTIPAYKDSV
                     AFEDYRLKEGDRLAIKVYSIDEKTNTLFNGVNNFESGFYGGSLNSDLYTYLIKPDGTI
                     SLPMVGKVFVEGQTQREAKKTIENAIQSVIKINTVDVKLLEKYYSVISSSTSGRFPIT
                     REKINIFEALAMAGDIGTYGDRSKIRIIRETENGTQIKMFDVRSADIIHSEFYYVEPN
                     DVIYIQDVNEQFFSVTSFPSLLATLISTFSLAIFIYDLVK"
     gene            2788..3576
                     /locus_tag="PLR72_02200"
     CDS             2788..3576
                     /locus_tag="PLR72_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOK36056.1"
                     /translation="MINKILHFFYNIKITENFVRLFLTVLPFLCLTSCYNYKSVGLLQ
                     ENNHSLPQYEKQLYEDYKLCVNDEIVFRLITTDETISKLISSNSPRVGQQISYRVYPD
                     GTIDIPFLSQIPVKGLTLNEATEVIQTRIREIIPDAEIKLSLANKSFTVIGDIGSGTF
                     PIYKEKMTIFQALALSGDLQLSGDRKHVRILRNIEGKTNILEFDIRPASIVESKYYYI
                     YPNDIIYVQRAPSSFYKVNNYNSFLGLITTSLTFLISVIYLLKL"
     gene            3606..5954
                     /locus_tag="PLR72_02205"
     CDS             3606..5954
                     /locus_tag="PLR72_02205"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOK36057.1"
                     /translation="MDIEQKNHTQAFVDEESSFDIREWLHYFLQHWYLFLCFVILFIM
                     LIYAKNRRWMPLYETAGTIIMAENKYLPQNQFFLGGFNVGGYQNVNDQLVILNSYDLI
                     GKVVDSLEFMKVDYLTIGRFKTRNIYRNTPIYIQSNYISPDAYQTVFKIKLFSDNSFI
                     ITPYKNKNFGNFNIEGHFGEPIHHNLFFITVNKLTNIPYSGEIEMYFRFRTRESLINE
                     FSSRLSSKYVMDGSSVLRVSLMGETPERDIDFLNKLFEVFLNDNLAKKNDVANKTIYF
                     INEQLDILSQSLAFSENKLTSFKQSNQIIDISSHASEIINKLNFYEEKNNQIKLKETY
                     LNYLSDYLKTNFEEGKVIAPSNLGLNEPILMQLVQQINDLYLQRSELSPKNIYYEKYT
                     NEIENVKTAIFELIKSIRTSLKIEKDENNRQIALLNNEMKKLPEKELAMNAIERDYKL
                     NDNYYTFLLQKKAEAEIQKASNSPGNSVLENARIISITNNKEKSKTTTRYLILAFLIP
                     SLIIVLKKSLNNTITSITDIEKKSSFPVIGVVLHTKNDDPLLCAKYPNSLFSEMFRII
                     RTRIEFIAQRKKNIVVLVTSLTSEDGKTFFSTNLAAIYGMTGEKTLLIDMDIRKPSVN
                     KRLNVSHPKGVTNYLIGECTLPEIINKIDNVNFDIIFAGTVPPNPGELIRSEKLIKMF
                     EELKKQYDYIIIDVSPVGLVSDTYSLVPLSDVNLLVVRNKKTNKTFFKNIATQLMEDH
                     IQNFYVILNDVDTSYGGYEKSYKYHMYDKKTDNKYYDMIDEI"
     gene            5984..7135
                     /gene="gmd"
                     /locus_tag="PLR72_02210"
     CDS             5984..7135
                     /gene="gmd"
                     /locus_tag="PLR72_02210"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOK36058.1"
                     /translation="MNKKVALITGITGQDGAYLAELLLSKGYIVHGIKRRSSLFNTER
                     IDHLYQDPHEKNRNFILHYGDMTDSLNLTRIIQETQPDEIYNLGAMSHVKVSFDTPEY
                     TANADGIGTLRMLEAIRLLNLTEKTRFYQASTSELYGLVQEIPQRETTPFYPRSPYAV
                     AKLYAYWITVNYREAYGLHASNGILFNHESPLRGETFVTRKITRAAARIALGLQDKLF
                     LGNLSSRRDWGHAKDYVYAMYLILLQDQPDDYVIATGKSTEVREFARLSFEQIGIKII
                     FVGNGVEEKGYIDKIDEDIFVEQVGEKFLTNVKNIKESVIEVDPNYFRPTEVDYLIGD
                     ATKAKTKLNWTPQYDLPALISEMMKSDVQLMKKEMHLKQGGFRILNHYE"
     assembly_gap    7136..7235
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7255..8322
                     /locus_tag="PLR72_02215"
     CDS             7255..8322
                     /locus_tag="PLR72_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOK36059.1"
                     /translation="MEKNSKIYVAGHTGLVGSAIKRNLEKNGFTNIVGRTQAELNLLK
                     EAPTEAFFKEEKPEYVFLAAAKVGGIVANNTFRAQFIYENLMIQNHVIHQSYLYGVKK
                     LLFLGSSCIYPRLAPQPIKEEYLLTGPLEYTNEPYAIAKIAGIKMCEAYHTQYGCNFI
                     SIMPTNLYGPNDNYDLEKSHVLPALIRKMVLGKYLMENDWENLRTDLTRLPIEGVNGS
                     YSKEIILKVLEKYGIIHQNGKVVITLWGTGSPLREFLHVDDMADATVFLMQHYDAPDS
                     IPSHVNVGCGEDISIKELSGIVKKNVGYEGEIQWDTTKPDGTPRKLLDVTRLNSLGWK
                     PKISLEEGIKQVVANYTNYIH"
     gene            8371..9711
                     /locus_tag="PLR72_02220"
     CDS             8371..9711
                     /locus_tag="PLR72_02220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOK36060.1"
                     /translation="MKIAIVGTGYVGLVSGTCFAEMGVDVTCVDINQEKIDQLKKGVI
                     PIYEPGLDDMVSRNIQAGRLHFTTQPTDVLNEVEAVFIAVGTPPKKDGSADLKYVFDV
                     AHTIGQNLQQYLLVVTKSTVPVGTTFNVKKIIQEELNKRGVDIDFDVASNPEFLKEGD
                     AINDFMSPDRVIVGVENERSKEILTKLYRPFLLNNFRVIFMDIPSSEMTKYAANAMLA
                     TRISFMNDVANLCEKVGADINMIRKGIGTDQRIGNKFLYAGCGYGGSCFPKDVRALIR
                     TAEENDYEMRILKAVDEVNERQKEVLYYKLLKHFDDLHGKTIALWGLSYKPNTDDIRD
                     ATSLVIIEKLLKASCKIRAYDPVAMPETQKYISEKMKDLLTSENTVYFGKDIYDTVLD
                     ADALLLITEWKEFRMPSWNVVKRAMKFPLVIDGRNIYVKEELKGLGFEYEGIGN"
     gene            9751..10581
                     /locus_tag="PLR72_02225"
     CDS             9751..10581
                     /locus_tag="PLR72_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK36061.1"
                     /translation="METVIKPKNSLLEINLNEIWQYRDLLFMFVKRNIITQYKQTILG
                     PTWFFIQPALTTLMYMIVFGGIAKIPTDGLPQPMFYLAGVVCWQYFADCLTKTSNTFI
                     DNQGIFGKVYFPRLIVPLATVISNLVRMLIQFLLFIAVYIYYIVVGVKVAPNLYILLV
                     PLLIIMLAGLSLSFGIIISSLTTKYRDLTILFNFIVQLWMYATPIIYPLSTMPPNRQW
                     IMALNPVTSIIETFKYGTMGVGTFSWAHLSYSFVFMAVLLFVGIMVFNKVQRSFMDTI
                     "
     gene            complement(10631..12715)
                     /locus_tag="PLR72_02230"
     CDS             complement(10631..12715)
                     /locus_tag="PLR72_02230"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK36062.1"
                     /translation="MQDAKVMNKQLNRHIKNFLFLFLIFFSSIVSAQLNTDRILTIGK
                     SAFYFEDYVLSIQYFNQVIKIKPYLAEPYMYRAMAKIQLGDYEGAEKDCNEAIERNPF
                     IPQAYYTRGFAKLKLNCFNEAAEDFSKALEFSPDNLYLIANRILAFEKAGNYEAALKD
                     INEFLKLSPNNTDMYYEKGRLQLALKDTIGAEKTFDKYIEMNPSSSLGWSARALLKLQ
                     RNDKEGALKDYNEAIKRNSTFAGDYINRAFLNIEKNNFRQALEDYDKAIALDSKNELA
                     YFNRGLLRAKLGDTNNALNDFKKVLALNPSSPEALWQKAILENTLGNYEEAIKNYQQL
                     INNHPYFLPAFLGIAEAYQKLGDEKNAFRYKQLAYNLETNKDKIKKEQITASTKIVQN
                     TQNNGKQRNIEIFNRFITQNASENETNSKYNNSLRGTIQDKFVDIVNEPNFILTYYSK
                     PEEIRRTNLFHLAVEEYNLQKKLSSPLKITNYEIPLTPELINQHFEKINSLSFQLEKN
                     NQDADLYFSRALEFSLIRDYTSAIEDLNKAISLRPNFMLAYFTRANIRYKLIESDNQL
                     NKSANTLTAGSETSSNKMKEQQNKLDFELILRDYDKVIQLTPDFTFAYYNKANILCNQ
                     KDFSTAIIYYSKVIELDRDFAEAYFNRGLSYLFLGEDNKGLNDLSKAGELGIYKSYNI
                     IHRFKQQSQEGE"
     gene            complement(12690..14996)
                     /locus_tag="PLR72_02235"
     CDS             complement(12690..14996)
                     /locus_tag="PLR72_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOK36063.1"
                     /translation="METENLNINKKDKFKKIFWRIFGVFILSIGLLFTCISLGWIGYL
                     PPLEELQNPQNKFASEIYSEDLQLLGQFFKNKENRVVVHYQDISKNVINALVATEDKR
                     FYKHSGIDGRAIVRVIILNGIFRQKSAGGGSTLTQQLAKQLYSPPANNIIERALQKPI
                     EWVIAVKLERLYTKEEILTMYLNQFDFLNNAVGIKSAAQTYFGKIPAELNIEEAATLI
                     GMCKNPSYYNPVRYNERSRERRNMVLYQMYKNHYLSEEQYDSLKQLPLVLHFQRIDHK
                     EGLAPYFREYLRITLMAKEPQRSNYASWQEQQYLDDKWAWDNDPLYGFCNKNLKPDGS
                     PYNIYTDGLKIYTTIDSRMQRYAEEAVNEHIQELQKLFFQEKKGRSYAPFSRSLSQQE
                     IEDILNRAIKQSERYQKMKADGKSEAEIQKSFRTPVDMKVFSYQGDIDTIMSPLDSIR
                     YHKHFLRCGFMSMEARTGHVKAYVGGPNFSRFQYDMVKKGRRQIGSTIKPFLYTLAME
                     EGMWPCDKTINQPITLIAETGQPWTPRNSSKARIGEVVTLRWGLANSNNWISAYLMSL
                     FTPQSLVDLMHSFGIRGKIDPVVSLCLGPCEVSVEEMVDAYTAYTNKGIRVEPLYVTR
                     IEDNNGNVIANFTPKMHEIFGETTAYKMIDMLQAVVNEGTGSRVRTRYGLTMPMGGKT
                     GTTQNNSDGWFMGFTPSLVSGVWVGGEERSIHFDRMSEGQGATMALPIWALYMKKVLS
                     DADLKYSPNEQFEIPKWFNPNAGCEGNE"
     gene            complement(15042..16172)
                     /gene="hemW"
                     /locus_tag="PLR72_02240"
     CDS             complement(15042..16172)
                     /gene="hemW"
                     /locus_tag="PLR72_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOK36064.1"
                     /translation="MAGIYIHIPFCKKRCIYCDFYSSTHQEYIPQLIDAIIKELKGRK
                     NYLQNEMVKTIYFGGGTPSLLTENELGKILAAIFESYSVEETAEITLEANPDDLISSE
                     YLSSLRKLKINRLSIGIQSFDDEKLKFLNRRHTAGQAIQAVSNAHEVGFNNISIDLIY
                     AIPGQTLQQWEKELNIAFSLPVQHLSAYELTYEKQTLLGKRKQKGLTTPVDEEISIKM
                     YETLLEEAKKHGFERYEISNFALPGFYSKHNSAYWKQKIYIGIGPSAHSYNSTSRQWN
                     ISSVEKYIKAIKHQEKYFEKEELTLNQQYNEYLMVSLRTAEGLDLNELEEKFGTELKN
                     ECLKSLKIFMNDKMVTQDNEKLCLTTKGIQISNLIISELMRI"
     gene            16295..16369
                     /locus_tag="PLR72_02245"
     tRNA            16295..16369
                     /locus_tag="PLR72_02245"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16330..16332,aa:Pro,seq:tgg)
BASE COUNT         5510 a         2508 c         2705 g         5656 t
ORIGIN      
        1 gataaatctt tttcaatata ggtgtcagta agaggtaaat aagcttctgg ttgataataa
       61 tcgtaatagg aaacaaaata ttctactgca ttatgaggaa agaatgattt gaattcactg
      121 taaagttgtg cagccaaagt tttattatga cttaatacca gcgtaggttt ttgtacctgt
      181 tcaatgacgt tagcaatagt aaaggttttt cctgaacctg ttacgcccaa taaagtttgg
      241 taaggaacat ttgcctttat tccttcaacc agttgttgaa tagcttccgg ttggtcacca
      301 gtgggacgat aaggcgattc aagtttaaaa agcataaaga aaagaaagtt tattgaaaaa
      361 tgaaaagtta taaaattatc tttgaaaaga actcatgttt ttaaattatt cttccgaaat
      421 aattatcaaa aaacatacat atcaagaaaa agttcaacga ttattccgtt ttttcttccg
      481 tctctttttc gaggggcatc accaaccaaa gaatgagata aaccaatata ccaggaaagg
      541 ctgcactaag aatggaaaga agcacataaa gaattctaac aatagttgga tcccagccta
      601 aaaatttagc tatacctcca caaactccag cgagtaactt atttttgttt gagcgggtta
      661 atcttttgct cataaaaatt cagtttttct gcaaagtaac aaaataaatg acaaaaaaaa
      721 tcaaaattac gcagataaaa atgattttgt ttcttatacc gaaaatattc ggggttcaca
      781 gtcggtgttg cggagaaaag tgaggatttt ttagtatttg taaaagatat tcaatattga
      841 attctaaagc gtaaattcaa gtaattgaca aaacaaactt ctaaaaataa gcttctaaaa
      901 aattttatta atttctttta ttgatctgtt caatttttaa ctgagctttt tgaatttcct
      961 ttttcaaacg attgatatct tttttcacta cccataaatg gtaggtttcc atgatggctt
     1021 ttttatcggc attttgcaaa aaatagctat aattctttct tccatgcaaa gtaactttta
     1081 aaggctcatt aatatattga gaaacaaaag cagatactcc attttcagct tcattcttga
     1141 aagtaacaat ttcccatcgc cctgtttcat ctgaaaaact taaataaaaa ggacttgaaa
     1201 tatcaattgt gtcagtatga gcaaaaactt caccgttaga tatttctaca gaattgtgct
     1261 cgatttttgg tccaacatat tggctaatca agtaataatc agcattctca tcaacataag
     1321 ctttcagata agtacgattg gcattggctt ctgttgattg ggtcttataa acatagttgc
     1381 caatttcttg atattttgga tttttttcca agcgaaaata ttgcaacagc gaatctgctt
     1441 ccctttcttt tataggtaaa aggctgtcac aataagcaat agttttacga cattctcttc
     1501 tggttattgt atctttgtat gcttcagcta ttcgccgttt agccaccagt cgaggatatt
     1561 tggcatgaaa gctgtcaata gttatttttg cagcatttaa ttcattttgc tggataagtt
     1621 gttcaatttc ttgaagatgt tgatcggctt ctttttcctg tttactgcgt gaattacatg
     1681 aaataaaaaa gaaaataata ctgacaatta ttaaatttag actgactttt ttatatgtca
     1741 tattgataaa tttaagagag aattaataaa taaaaatgta taatactgaa aatttattca
     1801 aaaaatttta ttcaaattta aaataatatg cgacgaaaaa aaatcaaaat attccctata
     1861 aaaaacaaaa atagtaagtt ttatttaatt ctcaaaaaga gatagatagt tactaaataa
     1921 gtcggtcgtt aagaaattaa tttttttatt tttactgctt tccatcttta taaattttca
     1981 atatctttgt tatattttta aagaatgggg taatatgcaa aaattttggt atctcacggt
     2041 ttttatgtta tggataattt ttcaatttac ttcttgtatc acagcaagaa aggtcaatta
     2101 tctacaggaa ccatctcaaa ccattccagc atataaagat agtgttgctt tcgaagatta
     2161 tcgtctaaaa gaaggagaca ggcttgctat caaagtatat tcgatagatg aaaagaccaa
     2221 tactttattc aatggtgtta ataatttcga atcgggcttt tatggaggtt ctctcaatag
     2281 tgatttatat acctatttga ttaaacctga tggcactatt tcattgccaa tggtaggaaa
     2341 agtttttgtt gagggacaaa ctcagcggga ggcaaaaaaa acaattgaaa atgctattca
     2401 atctgtgatt aaaattaata cggttgatgt gaaattgttg gaaaaatatt atagtgtcat
     2461 tagttcaagt acttcaggaa gatttcctat tacacgtgaa aaaattaata tttttgaagc
     2521 attggctatg gcgggcgata ttggaactta tggtgatcga agtaaaataa gaattattag
     2581 agaaactgaa aatggtactc aaatcaaaat gtttgatgta agaagcgcgg atattattca
     2641 ttcggaattt tattatgtgg agccaaatga tgttatttac attcaagatg taaatgagca
     2701 attttttagt gttacttctt tcccttcttt gttggctact ctcatatcaa ctttttctct
     2761 ggcaatcttt atatatgatt tagtaaaatg attaacaaga tattgcattt tttttataat
     2821 ataaaaatta cggaaaattt cgttcgttta tttttaaccg ttttgccttt tttatgtttg
     2881 acttcttgtt ataattataa gagtgttggc ttattgcaag aaaacaatca ttcattacct
     2941 cagtatgaaa aacaacttta tgaagattat aaactttgtg taaatgacga aatcgtattt
     3001 cgtttaataa ccacagacga aacaatttca aaactaattt cttctaatag tcccagagta
     3061 ggtcaacaaa tttcttatcg agtctatcct gatgggacaa tagatattcc ttttcttagt
     3121 caaatacctg taaaaggatt aactttgaat gaagctactg aggtaattca aactcgaata
     3181 agggaaataa tcccagatgc agaaataaaa ttaagtcttg ctaacaaatc ttttacagta
     3241 attggagata ttggaagtgg aacattccct atttataaag aaaaaatgac tatttttcaa
     3301 gctttagctt tatcaggtga tttacagttg tcaggtgata gaaaacatgt acgtatttta
     3361 cgtaatattg agggaaaaac aaacatactt gaatttgaca ttcgcccggc aagtattgta
     3421 gaatcaaaat actattatat ttatccgaac gatatcattt atgttcagcg tgccccttca
     3481 agtttttata aagtaaacaa ttataattct tttttagggc taattactac ttctttaact
     3541 tttttgatta gtgttattta tttattaaaa ttgtaattga ttaaattaaa aatttattta
     3601 gaaaaatgga tatagaacaa aaaaatcata cccaagcctt tgttgatgaa gaaagcagct
     3661 ttgatataag agagtggctt cattattttc tacaacattg gtatttgttc ttatgttttg
     3721 tgatactttt tataatgttg atttatgcta aaaacaggag gtggatgcca ttatacgaga
     3781 cagccggaac cattattatg gcagaaaata aatatcttcc tcaaaaccaa ttttttttag
     3841 gaggatttaa tgttggaggt tatcaaaatg taaatgacca acttgtcata cttaattctt
     3901 atgatttgat agggaaagta gttgatagcc ttgaatttat gaaagttgat tacttgacaa
     3961 tagggcgttt caaaactcgt aacatatatc gtaatactcc tatttatatc caatccaact
     4021 atatttcccc tgatgcctat caaacggttt ttaaaatcaa attattttca gataatagtt
     4081 ttattataac tccttacaag aataaaaatt ttggaaattt taatattgaa ggccatttcg
     4141 gagaacctat ccatcacaat ttatttttta tcactgttaa taaattaaca aatatccctt
     4201 attcggggga aatagaaatg tattttagat tcaggacacg cgaatcttta attaatgaat
     4261 ttagttcacg tttaagtagt aaatacgtaa tggatgggtc atcagtgctt cgggtatcgt
     4321 taatggggga aactccagaa agagatatag attttcttaa taaattattt gaggtatttt
     4381 taaatgataa tttagcgaaa aaaaacgatg tagctaataa aaccatttat tttatcaatg
     4441 aacaattaga tattttatct caatcattgg ctttttccga aaataaactt accagtttta
     4501 aacagtccaa tcaaataatt gatatttcaa gccatgcctc cgaaataata aataaattga
     4561 atttttatga agaaaaaaat aatcaaatta aattaaagga aacttatttg aattatttat
     4621 cggattattt aaaaactaat tttgaagaag gcaaagtaat agctccctca aatctgggct
     4681 taaatgaacc catattaatg caattggtac aacaaatcaa tgatttatat ttacaacgaa
     4741 gcgaattaag cccgaaaaat atttattatg aaaaatatac taacgaaatt gaaaatgtaa
     4801 aaacagctat ttttgaatta attaagagca tacgaacttc tttgaaaatt gaaaaagatg
     4861 aaaataatcg tcaaatagct ctattaaata atgaaatgaa aaaacttcct gaaaaagagt
     4921 tggcaatgaa cgcaatagaa cgcgattata aactcaatga taattattac acttttttac
     4981 ttcaaaaaaa agcggaagca gaaattcaaa aagcttccaa tagccctggt aatagtgttt
     5041 tggaaaatgc aagaattatt agcataacta ataacaaaga aaaatcaaag accacaacaa
     5101 ggtatttaat attggctttt ttgattcctt ctctgataat tgttttaaag aaatcattaa
     5161 ataacacgat tacttctatt acagatatcg aaaaaaaatc ttctttccca gtaataggag
     5221 tggttcttca tactaagaat gatgatccgc ttttatgtgc taaatatcca aattcattat
     5281 tcagtgaaat gtttagaatc attcgcacaa gaattgaatt tatagcccag cgtaaaaaaa
     5341 acatagtcgt attagttact tctcttactt cagaggatgg aaaaactttt ttcagtacca
     5401 atcttgctgc catttatgga atgactggag agaaaacatt attaattgat atggatatca
     5461 gaaaaccaag tgtaaataag cgtctaaatg tttctcatcc taaaggagtt acaaattatt
     5521 taattggaga atgcactttg ccagaaataa ttaataaaat tgataatgta aattttgata
     5581 taatttttgc tggaacagta cctcctaatc ctggtgaact gattcgttct gaaaaattaa
     5641 ttaaaatgtt tgaagaacta aaaaagcaat atgattatat aatcattgat gtaagcccag
     5701 taggactggt ttcagatact tattctttgg ttcctttatc agatgttaat ttattggttg
     5761 taagaaataa aaaaacaaat aaaactttct ttaaaaatat tgcgacacaa cttatggaag
     5821 accatattca aaacttttat gtcattttaa acgatgtaga tacaagttat ggtggatatg
     5881 aaaaatctta taaataccat atgtacgata aaaaaaccga taataagtat tatgatatga
     5941 tagacgaaat ttaattataa tccagataat caaaaattaa attatgaata aaaaagtagc
     6001 tttaattaca ggtataaccg ggcaagatgg tgcatatttg gcagaattat tattaagtaa
     6061 aggttatatt gttcatggaa taaaacgtcg ttcttcactt tttaatactg aacgaattga
     6121 ccatttgtat caggatcctc acgaaaaaaa ccgtaatttt attctacact atggtgatat
     6181 gactgactcc ttaaatttga cccgtattat tcaggaaacc caaccagatg aaatatacaa
     6241 tttaggagct atgagtcatg taaaagtaag ttttgatacc ccagaatata cagctaatgc
     6301 tgatggaata ggaacattgc gaatgctgga agccattcgt ttattaaact tgacagagaa
     6361 aacgcgattt tatcaagctt ccacctcaga gttatatgga ttggtgcaag aaattccaca
     6421 acgggaaacg actccttttt atccacgttc tccttacgct gtagcaaaat tgtatgccta
     6481 ctggattaca gtcaattata gggaagcata cggtttacat gccagcaatg gtattttgtt
     6541 taatcatgaa tcacctttgc gaggcgaaac ttttgtaacg cgaaaaataa cacgtgcggc
     6601 tgctcgaatt gcactgggac ttcaggacaa attgtttctt ggtaatttgt cttcccgtcg
     6661 tgactgggga catgcaaaag attatgttta tgccatgtat ttgattcttc tgcaggatca
     6721 acctgatgac tatgtcattg ccactggaaa atcaactgaa gtacgtgagt ttgctcggtt
     6781 gtcatttgaa cagattggaa taaaaatcat ttttgttggt aatggtgtcg aagagaaagg
     6841 atatattgat aaaattgatg aagatatttt tgtagaacaa gttggagaaa aatttttgac
     6901 aaacgtaaaa aatataaaag aatctgtgat tgaagtagat ccaaattatt ttcgtcctac
     6961 tgaagtagat tatttgattg gtgatgctac caaagccaaa acaaaactta attggactcc
     7021 tcaatatgat cttccagcat tgatcagcga aatgatgaaa tctgatgttc aattgatgaa
     7081 aaaagaaatg catttaaaac aaggaggatt cagaattttg aatcattatg aataannnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttttt ttactctaat tttaatggaa
     7261 aaaaattcaa aaatatatgt agctggtcat actggtttgg taggttcagc cattaaaaga
     7321 aatcttgaaa aaaatgggtt tactaacatt gtaggtcgta cccaagcaga attaaattta
     7381 ttgaaagaag ctcctactga agctttcttt aaggaagaaa aaccagaata tgtttttctg
     7441 gcagcggcaa aagtaggtgg cattgttgct aataatacat ttcgagctca attcatttac
     7501 gaaaatttaa tgatacaaaa tcatgtcatt catcaaagct atttatatgg agtaaaaaaa
     7561 cttttattct tgggctcgtc ttgcatttat ccacgtttag cacctcaacc catcaaagaa
     7621 gaatatttat taactggacc gcttgaatat accaatgagc cttatgccat cgcaaaaatt
     7681 gcaggaatta aaatgtgtga agcatatcat acacagtatg gatgtaattt tatttccatt
     7741 atgccaacca atttatacgg accaaatgac aattatgatc tggaaaaatc acacgtttta
     7801 cctgctttga ttcgaaaaat ggtattagga aaatatttaa tggaaaatga ctgggaaaat
     7861 ttaagaaccg atctaaccag acttcctatt gaaggagtga atggttctta tagtaaggaa
     7921 ataatattaa aagtattaga aaaatatggg attattcatc aaaatgggaa agtagttata
     7981 acgctttggg gaacaggttc tccacttcgg gaatttcttc atgtggacga catggctgat
     8041 gccactgttt ttcttatgca gcattatgat gctcctgata gtattccttc tcatgtaaat
     8101 gttggttgcg gtgaagacat ttctataaaa gaattatcag gaatagttaa aaaaaatgta
     8161 ggctatgaag gtgaaattca atgggataca acaaaacctg atggtacccc cagaaaattg
     8221 ctggatgtta ctcgattgaa ttctcttggc tggaagccaa aaataagttt agaagaagga
     8281 atcaagcagg tagttgcaaa ttacaccaat tatattcatt agaaatgata aaaacagttt
     8341 ttagtcaact taataaattt ttgaattaaa atgaaaatag ctattgtagg tacgggatat
     8401 gttggacttg tttcgggaac ttgttttgct gaaatgggtg tagatgtaac ctgtgttgat
     8461 attaatcaag aaaaaattga tcagttaaaa aaaggtgtta ttcctattta cgaacctgga
     8521 ctggacgata tggtctcacg taatatacag gcaggaagat tgcatttcac tactcaacct
     8581 actgacgtat tgaatgaagt agaagccgtt tttattgctg ttggtactcc tcctaaaaaa
     8641 gatggaagtg cagatttaaa atatgttttt gatgtggctc acaccattgg acaaaacttg
     8701 cagcaatatc ttttggtggt tacaaaaagt accgttccag taggtacaac ctttaatgta
     8761 aagaaaatta ttcaagaaga attaaataaa cgtggcgttg acattgattt tgatgtagct
     8821 tcaaatccag aatttctgaa agagggtgat gccattaatg attttatgag tccggataga
     8881 gtgattgtag gtgtggaaaa tgaacgatca aaagaaattt tgacaaaact gtatcgtccg
     8941 tttctcttaa acaattttcg agttattttt atggatatac cttcctcaga aatgacaaaa
     9001 tatgcagcaa atgctatgct tgcaacccgc attagtttta tgaatgatgt agcaaactta
     9061 tgtgaaaaag tcggagccga catcaacatg ataagaaaag gaataggaac cgatcaacga
     9121 attggcaata aatttcttta tgcaggctgt ggttatggcg gttcgtgctt cccaaaagat
     9181 gtaagagctt tgattcgtac tgccgaagaa aatgactatg aaatgcgtat tttaaaggct
     9241 gtagatgaag taaatgaacg tcagaaagag gttctctatt acaaattatt gaaacatttt
     9301 gatgatttgc atggaaaaac tattgcttta tggggattat cttataaacc caatactgac
     9361 gatatccgtg atgcaacgtc attagttata atagaaaaac ttttaaaagc aagctgcaaa
     9421 attcgggctt atgatcctgt agctatgcca gaaacccaaa aatatatttc tgaaaaaatg
     9481 aaagatttac taacatcaga aaatacagtt tattttggaa aagatattta tgatacagta
     9541 ttggatgctg atgcattgtt gttaatcacc gaatggaaag aatttcgaat gccaagttgg
     9601 aatgtagtaa aaagagcaat gaaatttcca ttggttattg atggacgaaa tatttatgta
     9661 aaagaagaat tgaaaggact gggctttgaa tatgaaggaa ttggaaattg agttaaagtt
     9721 tttattgaag gtaaaagagt tgttttttga atggaaacag taattaagcc caaaaatagt
     9781 ctgttagaaa ttaatctgaa tgaaatctgg cagtatcgag atttgctttt tatgtttgta
     9841 aagcgaaata ttatcacgca atacaaacaa actattcttg gtcctacatg gttttttatt
     9901 caaccagctc tcactaccct tatgtacatg attgtttttg gtggaattgc taaaattcca
     9961 actgatggac ttcctcaacc tatgttttat ttggctggcg tagtttgttg gcaatatttt
    10021 gcagattgcc tgaccaaaac atccaatact tttattgata atcagggaat ttttgggaaa
    10081 gtttattttc ctcgtttgat tgtaccattg gctactgtta tttcaaatct agtaaggatg
    10141 ttaatccagt ttctgctttt tattgcagtt tatatttatt atattgttgt tggagtaaaa
    10201 gtagccccca atctctatat tttattggtt cccttgttaa ttattatgtt ggctggtctt
    10261 tcactgagtt ttggaattat tatttcttcg ttaacaacca agtatcgtga tttgaccatt
    10321 ttgttcaatt ttattgttca actctggatg tatgcgaccc caattattta tccattaagt
    10381 actatgccac ccaatcgtca atggattatg gctttaaatc cagttacatc tattattgaa
    10441 acattcaagt acggaactat gggagttgga actttcagtt gggcacactt gagttatagt
    10501 ttcgttttta tggcggtatt gctgtttgtg ggtataatgg tgttcaacaa agttcaacga
    10561 agttttatgg ataccatata aaaaaactat tgcatcatcc tatcggttaa ttttgtataa
    10621 aattaagaat ctattcccct tcctgagatt gttgtttaaa tcggtgaatg atattgtaac
    10681 ttttatagat acctaattca ccagctttgc ttaagtcgtt cagtcctttg ttatcttccc
    10741 ctaaaaataa atagctcaat cctcgattaa aataagcttc tgcaaaatcg cgatctaatt
    10801 caatgacttt actgtaataa ataattgcag tagagaaatc tttttgatta cataaaatat
    10861 tagccttatt ataataagcg aaagtaaaat caggtgttaa ttgaattact ttatcataat
    10921 cacgcaaaat aagttcaaaa tccagtttgt tctgttgttc tttcatttta tttgatgaag
    10981 tttctgaacc agcagtcaaa gtatttgctg atttatttaa ttgattatca gattctatta
    11041 atttgtaccg aatattggca cgggtaaaat aagcaagcat gaaattgggt cgtaacgaaa
    11101 tggctttatt tagatcttca atggcgcttg tgtaatcacg tatcagcgaa aattcaagag
    11161 ctcgactaaa ataaaggtca gcatcctggt tgtttttttc cagttgaaaa gaaagagaat
    11221 taattttttc aaaatgttga ttgattaatt caggcgtcaa aggtatttcg taattagtaa
    11281 tttttaatgg tgaacttaat ttcttttgta gattatattc ttcaactgcc agatgaaaaa
    11341 gattggtacg acgaatttct tcaggtttgc tataataagt caatataaaa tttggttcat
    11401 ttacaatatc cacaaattta tcctgtatag ttccacgaag tgaattgttg tattttgagt
    11461 ttgtttcatt ttcagaagca ttttgggtaa taaaacgatt gaagatttca atatttcgtt
    11521 gtttcccatt attttgagtg ttttgtacta ttttagtgga agctgtgatt tgttcttttt
    11581 ttattttgtc tttattggtt tcaagattgt aagccaattg tttgtatcta aaagcatttt
    11641 tttcatctcc gagtttttga taagcttcag caatacctaa aaaagcaggt agaaaataag
    11701 gatggttatt aataagttgt tgataatttt ttattgcttc ttcataattg ccgagtgtat
    11761 tttcaagaat ggctttttgc cataaagctt caggacttga aggatttaat gctaaaactt
    11821 ttttgaaatc atttaaagca ttattggtat cccctaattt tgcccgtaac aaaccacgat
    11881 tgaaataagc taattcattt ttactatcca aagctatggc tttatcataa tcctccaaag
    11941 cttggcgaaa attattcttt tcaatattta aaaaagctct attgatataa tctccagcaa
    12001 aagttgaatt tctttttatg gcttcattgt aatcttttaa agctccttct ttgtcatttc
    12061 tttgtaattt tagtaatgct cttgcactcc atcctaagct actggaagga ttcatttcaa
    12121 tgtatttatc aaaagttttt tctgcaccaa ttgtgtcttt caaagcaagt tgcaatctac
    12181 ctttttcgta atacatgtcg gtattattag ggctaagttt aagaaattca tttatatctt
    12241 ttaaagcggc ttcataattt ccagcttttt caaaggctaa tattctattt gcaatcaaat
    12301 acaaattgtc gggactgaat tcaagtgctt ttgaaaaatc ctcagcagct tcattaaaac
    12361 aatttaattt taatttggca aaaccacgag tataataggc ttgaggtata aatggatttc
    12421 gttcaatagc ttcgttacaa tctttttcag caccctcata atctcccagt tgaatttttg
    12481 ccattgccct gtacatgtaa ggctctgcta aataaggctt gattttaata acttgattaa
    12541 aatattgaat cgaaagaaca tagtcttcaa aataaaatgc acttttgcca atggtaagaa
    12601 tacgatcggt attcaattgt gctgaaacaa ttgaagaaaa gaatatcaag aataaaaaaa
    12661 gaaaattttt tatatgtcta ttcaattgtt tattcattac cttcgcatcc tgcattggga
    12721 ttaaaccatt ttggaatttc aaattgttcg tttggtgaat attttaaatc agcatcagag
    12781 agaacttttt tcatatataa agcccaaata ggtagagcca tagtagcacc ttgaccctcc
    12841 gacatcctat cgaaatgaat agagcgttct tcaccgccta cccaaacgcc cgaaaccaat
    12901 gatggcgtaa agcccatgaa ccatccgtcg gaattgtttt gcgttgttcc tgtttttccg
    12961 cccattggca tagtgagacc gtaacgagtg cgcacccttg aacctgttcc ttcatttact
    13021 acggcttgta gcatatcaat cattttataa gctgttgttt ctccgaaaat ttcatgcatt
    13081 ttaggagtaa aattggcaat tacatttcca ttattgtctt cgatacgtgt tacatacaat
    13141 ggttcaacac gaataccctt attggtatag gcagtataag cgtcaaccat ttcttctact
    13201 gaaacttcac acggacctaa acacaaagaa acgacaggat caatttttcc tctaatgcca
    13261 aatgaatgca tcaaatctac gagagattgt ggagtaaata aactcattaa ataagctgat
    13321 atccagttat ttgaattagc caagccccaa cgtagtgtta ctacttctcc tattctggct
    13381 tttgaagagt ttcgaggagt ccagggttgt cctgtctcag caattaaggt aatgggctga
    13441 ttaatagttt tatcacaagg ccacatacct tcttccattg ctaaggtata taagaagggt
    13501 tttatggtag aacctatttg tctgcgtcct ttctttacca tatcatattg aaaacggcta
    13561 aaatttggtc cacccacata agcttttaca tgacccgtac gagcttccat agacataaat
    13621 ccgcatctta aaaaatgttt atgataacga atagaatcta aaggagacat tatagtatca
    13681 atatcgcctt gataagaaaa aactttcata tctactggcg tacgaaatga tttttgaatt
    13741 tctgcttcac tttttccatc cgctttcatt ttctggtatc gttcagattg cttgatagct
    13801 cggtttaaaa tgtcttcaat ttcttgttga gaaagtgagc gggaaaaagg agcataactc
    13861 ctccccttct tttcttgaaa aaataatttt tgtaattcct gaatgtgctc atttacagct
    13921 tcttcagcat aacgttgcat gcgggaatca atcgttgtat aaatctttaa cccatcagta
    13981 taaatgttgt aaggagaacc atctggtttt aaatttttgt tacaaaagcc atataacggg
    14041 tcattatccc atgcccattt atcgtctaaa tactgctgtt cctgccaact tgcatagtta
    14101 cttcgttgag gttctttagc catcaaagtg atacgtaaat attctctaaa ataaggagcc
    14161 aatccttctt tatggtcaat acgctggaaa tgcaacacca acggaagctg ttttaatgaa
    14221 tcgtattgtt cttcgctcag atagtggttt ttgtacatct ggtataaaac catattgcgt
    14281 cgttcacgag accgttcgtt gtaacgtacc ggattataat acgaaggatt tttgcacata
    14341 cctattaaag tggcagcttc ttcaatatta agttctgcag gaatttttcc aaaataagtt
    14401 tgtgcggctg attttattcc aaccgcattg ttcaaaaaat cgaactgatt aagatacata
    14461 gtcagaattt cttctttcgt gtaaagtcgt tccagcttga ccgctattac ccattcgata
    14521 ggtttttgta aagctctttc tataatgtta ttggctggcg gagaataaag ttgttttgcc
    14581 agttgctgag ttaaagtact tcccccacct gcactttttt gtcggaaaat accattcaaa
    14641 ataataacac gaacaatcgc tctaccatca attcctgaat gtttataaaa tcttttgtct
    14701 tctgtggcta caagagcgtt aataacattt ttggaaatat cctgataatg aactactact
    14761 cgattttctt tattcttaaa aaattgtccc agcaattgca aatcttcgga gtaaatttcg
    14821 gaagcaaatt tgttttgagg attttgcaat tcttccagcg gaggcaagta accaatccac
    14881 cctaaactaa tacaggtaaa tagtaatccg atactcagga taaaaactcc aaaaattctc
    14941 caaaatattt ttttaaattt atctttctta ttaatattca gattttctgt ttccatgctt
    15001 tcataaaata aaatacaaaa atagtgtttt ttttcaaaag gttaaattct cattaattca
    15061 cttatgatta gattggaaat ttgaatacct tttgttgtta aacaaagttt ttcgttatct
    15121 tgagttacca ttttatcatt cataaatatt tttagacttt ttaaacattc attttttaat
    15181 tcagttccaa atttttcttc caattcattc aaatctaatc cttcagcagt tcttaatgaa
    15241 accatcaaat attcattata ttgttgattc aaagtaagtt cttctttttc gaaatatttt
    15301 tcctgatgtt taatggcttt tatatatttt tctacggatg atatattcca ttgccgagaa
    15361 gtgctattat atgaatgagc tgaaggtcca atgcctatat aaattttttg tttccaataa
    15421 gctgaattat gtttggaata aaaaccaggc aaagcaaaat tggaaatctc gtaacgttca
    15481 aagccatgtt tttttgcctc ttcaagcaat gtttcataca tttttattga aatttcttca
    15541 tctactggag ttgtcaatcc tttttgcttt cgttttccaa gtaaagtttg cttttcgtaa
    15601 gtcaattcat aagcagaaag atgctgaaca ggcaacgaaa aagcaatatt caattctttt
    15661 tcccattgtt gaagtgtttg accaggtata gcatatatca aatcaatact aatattattg
    15721 aaacccacct catgagcatt acttacggct tgaatagctt gtccagcagt atgtcttctg
    15781 ttaaggaatt tcagtttctc gtcatcaaac gattggattc ctatactcaa acgattaatt
    15841 ttcagctttc ttagagacga aagatattca ctacttatta aatcgtcagg attggcttcc
    15901 agggtgattt cggctgtttc ttccacagaa tagctttcaa aaatagccgc aagaatcttg
    15961 ccaagttcat tttcagttaa taaactgggt gtacctccac cgaaatagat ggtttttacc
    16021 atttcatttt ggagatagtt tttccttccc tttagttcct ttattatagc atcaatcagt
    16081 tgcggaatat attcttgatg agtagaagaa taaaaatcac aataaataca ccgtttttta
    16141 caaaatggaa tatgaatgta aatgccagcc atataatttt gtcaggaaat tgaaaacttc
    16201 gataaaatgc tttctttgta acacaaattt actgtaaatt gtttggattt tcgataaaag
    16261 ctgttaactt tgcagtcgct tttaagaatc ttgacgggat gtagcgcagt ccggttagcg
    16321 cacctgcttt gggagcaggg ggtcgtgggt tcaaatcccg ctatcccgac acagaaaatg
    16381 aaagatgtac caaaaaattg aaaggtgcat cttttttatt tcgtaacgga ttttcctttc
    16441 caaattttgt aaaaaatgaa ccgaaagcca ataccaatc
//
LOCUS       DAOSAS010000027        16161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_2485_length_16161_cov_13.575376, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000027 DAOSAS010000000
VERSION     DAOSAS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16161
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2485_length_16161_cov_13.575376"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..2743
                     /locus_tag="PLR72_02250"
     CDS             356..2743
                     /locus_tag="PLR72_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOK36065.1"
                     /translation="MSKKENFKTWFYYALMLILFCVFVYFLIQKGNAIEAQYQTAHKI
                     QDTISQTQHFSTWKNFKLSVNQNLGESITLLLLQIIAILLVSRFFGYLFSKINQPTVI
                     GEILAGIVLGPSLLGHFSPAAYQFLFPAHSLTNLYLLSQVGLILFMFIIGMELNINAI
                     KNRTSQIIVISHASILIPYFLGMLLAYYFYEKFAITYTRFLPFALFIGVSMSITAFPV
                     LARIVQEKGLTKTHLGGISIASAAIDDVTAWCILAVVIAISKTGNFFSALYTILLAIA
                     YILFMLLIVRPFLRRVGQIYQNIETFNKNIVGFLFLMLLSSAFITQFIGIHALFGAFL
                     AGVVMPPIPNFRKLIIDKIEDVSVSLFLPLFFVYTGLRTEIGLLNTPYLWGICLIFIL
                     VGIIGKFVGGAFTARFMGESWRDSLSLGILMNTRGLMELIVLNIGYEMNILPPTIFVM
                     LVIMALVTTFMTTPILNLINRLFPEKNIIEEYKRQQAQGIYKVLIAVGNPENGKILLR
                     VAKKVLEGVKNTLSITVLHITPSTDTNPIYGEQFAEESFKAVKEEAEILQLPIETVYK
                     VTDNIEAGIVRLVNQEIFDFLLVGAGPSLSGIPFFKEKNVFRRIKWLNKFINKVSQQR
                     VVFYPGNLIKDKTKYFIEQSNCNVGVFINRGFTDITSTIVLLYDKEDEFLLRYARRIL
                     KNNNNATVQIMDVNKVIDNNESVARYINEMEKQFPNAVRILKQNKINSNTFSKYSFML
                     ISYQTWNKLVEIDKNQLANIPTTLIINKKISHFYSDGRRKQIVSLPDNETLNEDN"
     gene            2943..4076
                     /locus_tag="PLR72_02255"
     CDS             2943..4076
                     /locus_tag="PLR72_02255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK36066.1"
                     /translation="MKIIIVGTAYPFRGGLAAFNERLAKEFVKEGNEIEIMTFTVQYP
                     DFLFPGKTQFSSEKPPEGLKITRRINSIDPFNWRKVGKEIKSKSPDKVIFSYWTSFIA
                     PCFGTIAKFAHSPKTKMIGLIHNMISHESTFLDRWLPSYFVKYMDGFVAMSQTVIKDI
                     RLFDKQNKPIAISPHPIYDHYGELVPKKEAAAKLGLHEQKHYILFFGFIRYYKGLDLL
                     LEAFADKRLEELDVHLIIAGEFYEHPEPYLKQIQRLNLENKVELRSNFIPDNDVKYYF
                     SIADLIVQPYRSATQSGISQIAYHFEKPMLVTNVGGLAEIVPHQKVGYVVEVNPQKIA
                     DAIIDFYTNNRSEEFINNIKVEKSKYSWSGMTSTINMLFEETQ"
     gene            4108..6720
                     /gene="mutS"
                     /locus_tag="PLR72_02260"
     CDS             4108..6720
                     /gene="mutS"
                     /locus_tag="PLR72_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOK36067.1"
                     /translation="MQENIVETPLMKQYFKIKAKHPDAILLFRCGDFYETYSSDAIEA
                     SEILGITLTRRANGAAKSVEMAGFPHHALDTYLPKLVRAGKRVAICDQLEDPKLAKTI
                     VKRDVTELVTPGVTYNDTVLNFKENNFLASVYINKSQIGVSFLDVSTGEFLVAEGNGE
                     YVDKLLNSFSPKEILFDRNRKKEFEDLFGTKFYTYPLDDWVYTPESAYERLLKQFETT
                     SLKGFGVANLSNGIVAAGAILHYLDITHHQQTAHITHLLRIEEEQYVWLDRFTVRNLE
                     LFESINEGGRSLCDIIDKTISPMGARLLKRWIAFPLKNVQQINERLNVVEYFFKHPDV
                     KLQIEENIALIGDLERLVSKVAVGRINPREVVQLKVALKAIEPIKKICSATDNVTLKT
                     IGDQLNPCSIISERIEKEILNDPPILLNRGNIIQKGIDQELDELRELAYSGKDFLQKI
                     QERESKATGIPSLKIGFNNVFGYYLEVRNTHKDKIPTHWIRKQTLVNAERYITEELKI
                     YEEKILGAEEKIIAIETKLFNDLIISLLDYIPAIQLDSNLIAQLDCLLSFAKVSADNR
                     YMKPEINDDDIIEIKQGRHPVIEKQLPLGETYIPNDIYLDSNTQQIMMITGPNMSGKS
                     ALLRQTALITLMAQMGCFVPAENAKIGVVDKIFTRVGASDNISLGESTFMVEMNEAAS
                     ILNNLSNRSLVLFDELGRGTSTYDGISIAWAIVEYIHEHPDHRAKTLFATHYHELNEM
                     EKSFSRIKNYNVSVKEIDNKVIFLRKLVRGGSEHSFGIHVAKMAGMPQSIVKRAEQIL
                     KQLETDNRQSDIAKPVDKIAQQREGYQLTFFQLDDPVLEQVRDEIKNLDINNLTPLEA
                     LNKLNEIKRIVTGK"
     gene            7095..7559
                     /locus_tag="PLR72_02265"
     CDS             7095..7559
                     /locus_tag="PLR72_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOK36068.1"
                     /translation="MALPRLFTSRSNWTPSLPSLIDRFFDSDLMDWDTFNFAGENMTL
                     PAVNVKENDNEFKIEVAAPGLNKEDFKVNYDNGILTISSEKQEEKEEKEGEKVTRREF
                     SYQSFQRSFSVPEDIINADKISAKYDNGILYVTLPKHEEAKKAKNVKEIKIS"
     gene            complement(7706..9523)
                     /locus_tag="PLR72_02270"
     CDS             complement(7706..9523)
                     /locus_tag="PLR72_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOK36069.1"
                     /translation="MKKKNIRNKILLFLLIFPVSSMANDVPILANAYARNVISLDGKW
                     NYIIDPLENGYYDYRMQPYEKNGFFENKKAKNPQELVEYNFDTSPLMDIPSDWNTKNT
                     QLFFYEGTIWFKKDFTFHKSTDTRTILYFGAVNYDTKIYLNGKKVGEHIGGFTPFNFD
                     ITNEIKEGNNFLILKVDNKRQKEGVPTLNMDWWNYGGITRDVMLITVPEIYIEDYAVQ
                     YKTDKNGTIYGWIKLNKPLSDQNLFFEIPELKIKQSLTTNEEGIVNFEIKSHPKLWSP
                     TSPKLYEVKFSTDKETFSDQIGFRTIETKGKEIFLNGKKVFLKGISIHEEAPFRQGRA
                     WSKEDAKILIDWAKELGCNYVRLAHYPHNENMVREAERAGLMVWSEIPVYWTISWGNS
                     ETYKNAERQLHDMIYRDKNRCAVIIWSIANETPQSKERDLFLSSLSKYAKSQDSTRLI
                     SMAMEVTSSNDNVYTVKDNMNQYVDIISFNEYIGWYNGTPDLCRLVKWEIPYNKPVII
                     SEFGGGALQGLYGDKSERWTEEYQEELYLKNLEMLEKIDALAGISPWILVDFYSPRRQ
                     LSGIQDFFNRKGLISNNGIKKKAFYILQEWYKKIDEKYK"
     gene            complement(9541..12438)
                     /locus_tag="PLR72_02275"
     CDS             complement(9541..12438)
                     /locus_tag="PLR72_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOK36070.1"
                     /translation="MKNIFIYFFFFFLMISLNFLFCPKIYAEWNSFIINYDKNLYGKG
                     SQTWQIVPYDTRWVYFANKNGLLEFNGILWNLFPLNTGSDARSVYPSKRQQRIYVGSI
                     NELGYFEPNEKGKLNYICLSDSFDIANLQIGNIWKIYENDNILYFQGDNKIIKYLNGK
                     FFPIEAHRKIECSDLVNGVIYIGTEEGVWVLVGNTFSPLQGAESLISKRIRGVVPYGK
                     GILIVTAYDGLYYYDEHTLKPYLTGFENFMKQNEVFCAASSEQLIALGTIHGGILLIN
                     NQNHTVKYFNENNGLQNNTVLSLTFDKWNNLWAGLDNGIDYICLNSPFTNLYSYPYSY
                     GTGYAALLSDNYLYLGTNRGLYYTKYPVEMVEGQPNISPVPSSSGQVWDLCNIDNNLF
                     CCHDRGIFQIKGTNLHRIGNITGVWSCKPISGRKDRIYAGTYDGLYLLEKKDNKWKVM
                     RKIEGFFDSCRFFEEETSNILWIFNTEKPIKITLDSSLTQVVNIKKYDTQNGLPCNRN
                     IYISKIKGEIYFATPKGIYQYDQKIDSIYPAKEINQMLDGEKDYSRLIEFNHHLISIN
                     HKEICISEFPFKSGNKSRIIPIEFQTMDLVHGAENIIPINDSSFVIPNEYGFAFLKVP
                     SDYKTNENSCISLTINKVYISHPKDSLIYLDNFLHKKITPKIPFSHNTIRIEYGVSSF
                     TQRTDFNYQYRLNNEHWSDFTNSNTKEYSKLHEGTYTFEVRILLEDGKIKSDNFSFII
                     LPPWYRSKIAYLFYFIILIFCLFGIYKWDDARIKKKKLQATLAKDEELKNMEKEFEEE
                     NKRKEQQIIQFEKEKLEHDLQYKSQEMANLMINLVRKNEILTEIKSDLFKIKSSLKFR
                     ENKEIERMLLLTNNKIDSNIKSDEILKQIEEQFDLIHNNFMKRLQEKYPDLSVNERMM
                     CVYLKMNLSTKEIAPLLNISIRGVETLRYRLRKKFSLSRDENLIDYLNTKI"
     gene            complement(12652..12807)
                     /locus_tag="PLR72_02280"
     CDS             complement(12652..12807)
                     /locus_tag="PLR72_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36071.1"
                     /translation="MEIIFVPLLEPLASETIYGACIAIELKMMIKEHRKSFPSFNSER
                     NYQRNYF"
     gene            complement(12844..13125)
                     /locus_tag="PLR72_02285"
     CDS             complement(12844..13125)
                     /locus_tag="PLR72_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36072.1"
                     /translation="MVRKLVLDVENKIKANNVTVVKSEAIIRGQNVNGIEIICNGKKY
                     YSANLLICTGSRTAIPPICRLSEAGDSMVANHEMLKLKAQPPSSFCRWK"
     gene            complement(13184..14218)
                     /gene="hisC"
                     /locus_tag="PLR72_02290"
     CDS             complement(13184..14218)
                     /gene="hisC"
                     /locus_tag="PLR72_02290"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOK36073.1"
                     /translation="MNINKLLRENIKKLEPYSCARNDFKGEASIYLDANENPFNDPYN
                     RYPDPLQAELKEQFSKIKNISTSNIFLGNGSDEAIDLLYRAFCEPRIDNVVAIEPTYG
                     MYRVCAEINDVEFRKVLLNKKFDIEAYKLMDATNERTKIIWLCSPNNPTGNSLDGEEI
                     RKILKWFRGIVVIDEAYIDFSNKESFLKEISQYPQLVILQTMSKAWGNAAIRLGMAFA
                     SEEIIQVLNKIKYPYNVNFLTQQHALKVLQDPFQMKKWVSILLEEREKLRNELSKLAI
                     VEEIFPSDANFLLVKVKDANMIYQYLIKKGIVVRNRHNMPLCTNCLRITVGTPDENNV
                     LINTLKKLYI"
     gene            complement(14249..15541)
                     /gene="hisD"
                     /locus_tag="PLR72_02295"
     CDS             complement(14249..15541)
                     /gene="hisD"
                     /locus_tag="PLR72_02295"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOK36074.1"
                     /translation="MKIFKYPEQEKWASILSRPVIDRRALFETVQKILNKVHKDGDQA
                     IKEYTALFDKVSLDTLEVTKEEINEAEKLVPKELKEAIEMARRNIWKFHSAQQTVFKE
                     IQTSPGVNCWQKALPIEKVGLYIPGGTAPLFSTVLMLGIPAKIAECKEIILCSPPTSN
                     GKIHPSILYAAKMAGIHRIFKIGGVQAIAAMAYGTETVPKVYKIFGPGNQYVTAAKQL
                     VSMEGVAIDMPAGPSEVAIIADESANPVFVAADLLSQAEHSVDSQCVLITTSEKLIQP
                     VLEEIERQKKQLLRKDIIQQSLENTRIILVKSIENAINMINEYAPEHLIIATRFPQEV
                     SLNIVNAGSVFLGNYAPESAGDYASGTNHTLPTNGYAKSYNGVNLDSFLRKITFQYLT
                     RDGLVNLSNAIILMAENEGLDAHSNAVKVRLDENNLNF"
BASE COUNT         5354 a         2648 c         2537 g         5622 t
ORIGIN      
        1 tataaagcga gtagaagata ttttgaacaa cagaccaaga aaaaaattaa atttcctgat
       61 atataataaa ctgccaaaaa tcctgtacat atattagcag attaatagga tgttatttca
      121 aatcaaatat ttcaatcaac cctccatatt ttaaaaaatg attaaccccg acgcaagcct
      181 cgaggaattc ttttgattaa attgagtatt taaggtggtt ttttatttaa tttttcaatt
      241 cctttttgtc aaccattcct aaatttatca ttaaatttgc aaaaagaaaa gaataagaat
      301 tttgaagtag gagaaatatt attactgaca gaattcttaa aatttgaggg catttatgtc
      361 aaaaaaagaa aactttaaaa cttggtttta ttatgccttg atgcttatat tgttttgtgt
      421 gttcgtttat ttccttattc aaaaagggaa cgccattgaa gcacaatatc aaactgcaca
      481 caaaattcag gatactattt ctcaaactca acatttttct acgtggaaaa attttaaact
      541 gtcagtcaac caaaatttag gcgaatctat cactctttta ttgctgcaga ttattgccat
      601 tcttcttgtc tcacgttttt ttggttacct tttcagtaaa atcaatcaac caactgtcat
      661 tggagaaatt ctggcaggaa tagtattagg accatcatta ttaggacatt tttctccagc
      721 agcttatcaa tttttatttc cagcccactc attgaccaat ctttaccttt tgagccaggt
      781 aggtcttatc ttatttatgt ttattatagg gatggaattg aatatcaatg ctataaaaaa
      841 tagaacaagc caaatcattg ttatcagtca tgccagcatt ttaattcctt attttctggg
      901 gatgttgttg gcttattatt tttatgaaaa atttgcgatt acttatacca gattccttcc
      961 ttttgctctt tttattggcg tttcgatgag catcacagct ttccctgtgc tggcacgtat
     1021 tgttcaggaa aaaggattaa cgaaaacaca tttgggagga atttctatag ctagtgccgc
     1081 tattgatgat gtaacggcat ggtgtatttt agcggtagtc attgctattt ctaaaacagg
     1141 caattttttt agtgcacttt atacaatttt gttagcaata gcctacatat tatttatgtt
     1201 gttgatagtt agaccatttt tacgacgggt aggtcagatt tatcaaaaca ttgaaacgtt
     1261 taataaaaat atagtaggtt ttttgttttt aatgttgctc tcttctgctt ttatcaccca
     1321 attcattggc attcacgcat tatttggagc ttttctggca ggcgtagtga tgccacccat
     1381 tcccaatttt agaaaactta taattgataa aatagaagat gtttctgttt cactttttct
     1441 gcctttattt tttgtttata caggtttacg cactgaaatt ggcttattaa atacacctta
     1501 cttgtgggga atttgcttga tttttatttt agttggtatt attggaaaat ttgtgggtgg
     1561 agctttcaca gcacgattta tgggagaatc gtggcgcgat agcttgtcgt taggtatctt
     1621 aatgaataca cggggattaa tggaactgat tgtattaaat attggctatg agatgaacat
     1681 tcttcctcct accatatttg taatgttagt aattatggct ttagtaacta cttttatgac
     1741 tactcctatt ctaaatttaa ttaatcggct cttccctgaa aagaatatta ttgaagaata
     1801 caaacgacaa caagcacaag gaatttacaa agttcttatt gcagtaggta atccagaaaa
     1861 tggaaaaata ttgctccgag ttgcaaaaaa ggttttagaa ggagttaaaa atacattgtc
     1921 tattactgtt ttacatatta ctccaagtac tgataccaat cctatttatg gagaacagtt
     1981 tgctgaagaa agtttcaagg cagtaaagga agaagcagaa atccttcaac ttcctatcga
     2041 aacagtttat aaagtcactg ataacattga agctggaatt gtacgactcg tcaatcagga
     2101 gatttttgat tttttattgg taggagctgg tccttcttta tcaggcatcc ccttttttaa
     2161 ggaaaaaaat gttttcagga gaataaaatg gctcaataag tttattaata aagtttctca
     2221 gcaacgagtc gttttctatc ctggaaattt aataaaagat aaaaccaaat attttatcga
     2281 acaaagcaat tgtaatgtag gggtctttat aaacagaggt tttacggata ttacttcaac
     2341 aatagttttg ctttatgata aagaagacga attcttatta cgctatgcaa gacggatttt
     2401 aaaaaacaac aacaatgcaa cggttcaaat tatggatgtg aataaagtga ttgataacaa
     2461 cgaaagtgtg gctcgataca taaatgagat ggagaaacaa tttcccaatg cagtcagaat
     2521 tcttaaacaa aacaaaataa attccaatac tttttcaaaa tacagcttta tgctgattag
     2581 ttaccagacc tggaataaac ttgtagaaat agataaaaat caacttgcca atattccaac
     2641 aactctcatt atcaataaga aaataagtca tttttatagt gatggacgtc gcaagcaaat
     2701 tgtttcgctt cctgataatg aaactttaaa tgaagacaac taaaaggaat ccagcaattt
     2761 atttttataa atttaaaaat gactattaca atttaaattg aaaataaaat agcttttaaa
     2821 ttttatccta atttcctttc aaaatttgta actttgtgac aatctcgtgg cttgcaacaa
     2881 tcttttttac aaaaatatca gcaaaagcta tcattgcaac atcataaatc aaaatttatc
     2941 cagtgaaaat cattatagta ggaacggctt atccttttcg gggaggtttg gcagcattta
     3001 acgaacgtct ggcaaaagaa tttgtcaaag aaggaaatga aatagaaatt atgaccttta
     3061 ccgttcaata tcctgatttt ttattccctg gaaagactca attttccagc gagaagcctc
     3121 ctgaaggatt aaaaatcact cgacgcatca attctatcga tcctttcaat tggagaaaag
     3181 tgggcaaaga aattaaaagt aaatcacctg acaaggtcat ttttagttat tggacttctt
     3241 ttattgcacc ttgttttgga accattgcta aatttgcgca ttctccaaaa acaaaaatga
     3301 ttggactcat tcacaacatg atttctcacg aaagtacctt tttggataga tggttgcctt
     3361 cttattttgt caaatatatg gatggttttg ttgctatgtc acaaaccgtt ataaaagaca
     3421 ttcgcttatt tgataaacaa aacaagccaa ttgccatttc accgcatcca atttatgatc
     3481 attatggtga attggttccc aaaaaggaag cagctgcaaa attaggactt catgaacaaa
     3541 aacattatat tttatttttt ggatttatac gatattacaa aggattggac ttgcttttag
     3601 aggcttttgc tgataaaagg ttggaagaat tagatgttca tttgattatt gctggtgaat
     3661 tttatgaaca tcctgaacct tatttaaaac aaattcaacg tcttaattta gaaaataaag
     3721 tagaattacg ttcgaatttt attcctgaca atgatgttaa atattatttc agtattgctg
     3781 acctgattgt gcaaccatat cgttcagcga cacaaagcgg cattagccaa atagcttatc
     3841 attttgaaaa gccaatgtta gttaccaacg ttgggggatt agctgaaatt gttcctcatc
     3901 agaaagttgg ttacgttgtc gaggtaaatc cccaaaaaat tgcggatgcc attatagatt
     3961 tttatactaa taaccgttca gaagaattca tcaacaacat aaaagtagaa aagtcaaaat
     4021 attcatggtc gggaatgaca tcaactatca atatgctatt tgaagaaacg caatagttag
     4081 aaaataattt tttgagaaac taagaatatg caagaaaaca ttgtagaaac acctctaatg
     4141 aaacagtatt ttaaaatcaa ggcaaagcac cctgatgcta ttttattgtt tcgttgcggt
     4201 gatttttatg aaacttattc ctctgatgct attgaagctt cggaaattct tggtatcact
     4261 cttacccgac gtgccaatgg tgccgctaaa agtgtagaaa tggctggatt tcctcatcat
     4321 gctctcgata cttatttacc aaaattggtt agagctggta aaagagtagc tatttgcgac
     4381 caactggaag atccaaaact tgctaaaact atcgtaaaaa gagatgttac agagctagta
     4441 acaccgggcg ttacttataa tgatactgta ttaaatttta aggaaaataa tttccttgcc
     4501 agtgtttata tcaacaaaag tcagattggc gtttcttttc ttgatgtctc aacaggtgaa
     4561 tttttagtag ccgaaggtaa tggagaatac gttgataaac tcttaaatag tttttcaccc
     4621 aaagaaattc ttttcgatcg taaccgaaaa aaagaatttg aagatttgtt tggaacaaaa
     4681 ttttatactt atccattaga cgattgggtt tatacacctg agtcagctta tgaacgattg
     4741 ttgaaacagt ttgaaaccac aagtctgaaa ggattcggtg ttgctaatct ttccaatgga
     4801 attgttgctg cgggagctat acttcattac ctggacatta cccatcatca acaaacggca
     4861 cacattactc atcttttacg aattgaagag gagcaatatg tctggctcga ccgctttacg
     4921 gtaagaaatt tagaactttt cgaaagcata aatgaaggtg gacgttccct ttgcgatatt
     4981 atagataaaa ccattagccc aatgggtgct cggcttctga aacgctggat agctttccct
     5041 cttaaaaatg ttcagcaaat taacgaacgt ttaaatgtgg tagaatattt ttttaaacat
     5101 ccagatgtaa aattgcaaat tgaagaaaat atagctttaa taggtgattt agaacggctg
     5161 gtatcaaaag tagccgttgg cagaatcaat ccgcgtgaag tggtacaact gaaagtggct
     5221 ttgaaagcta ttgaacctat taaaaaaatt tgttcagcta ctgataatgt cacgttaaaa
     5281 actattggtg atcaattaaa tccgtgttca attatttctg aacgtattga aaaagaaatc
     5341 ctaaacgatc cgcccatttt gttgaatcgt ggcaatatta ttcaaaaagg tattgatcaa
     5401 gagctggatg aattacgtga attggcatat tctggaaagg attttttaca gaaaatacaa
     5461 gaacgtgaaa gcaaagctac tggtattcca agcttaaaaa ttggtttcaa taatgtgttt
     5521 ggttattatc ttgaagtcag aaacactcat aaagataaaa taccaacaca ctggattcga
     5581 aaacaaacat tggtcaatgc agaacgttac attacagaag aattaaaaat ttatgaagaa
     5641 aaaattttag gagcggaaga aaaaataatt gctattgaga caaaattgtt caatgatttg
     5701 ataattagct tgcttgatta cattccggct attcaattag attctaatct gattgctcag
     5761 ttggattgtc tgctttcttt tgcaaaggtt tctgctgata atcgttatat gaaacccgaa
     5821 attaatgatg atgacattat tgaaattaag caaggaagac atcccgtcat tgaaaaacaa
     5881 ttgccactgg gagaaacgta tatcccgaat gacatttacc ttgacagcaa tacccagcaa
     5941 attatgatga ttactggacc caacatgtca ggcaaatcag ctttgctccg acaaactgcc
     6001 ttgattactt taatggctca aatgggatgt tttgttccag cagaaaatgc aaaaattggt
     6061 gtagtggata aaatttttac gcgtgttgga gcaagcgata acatctcgtt gggagaatct
     6121 acttttatgg tggaaatgaa cgaagctgcc agtattttaa acaatctctc gaatcgaagt
     6181 ctggttcttt ttgacgaatt aggaagagga acaagtactt atgatggaat ttctatcgcc
     6241 tgggcaattg tagaatatat ccatgagcat cctgatcatc gtgctaaaac actttttgcc
     6301 acacactatc acgaattaaa tgaaatggaa aaatcatttt cacgtattaa aaactacaac
     6361 gtttcagtaa aagaaataga taataaggta atttttttac ggaaattagt tcgtggcggt
     6421 agtgaacaca gttttggtat tcatgtggct aaaatggcgg gcatgcccca aagtatcgtt
     6481 aaacgagctg aacagattct caaacaactt gaaaccgata accgtcagtc ggatattgcc
     6541 aaaccagtag ataaaatagc tcaacaacgc gaaggttatc aacttacatt ttttcaactt
     6601 gatgaccctg tattagaaca agttcgagat gaaattaaaa atcttgatat aaataattta
     6661 acacctctcg aagccttaaa caaattaaat gagataaaac gaattgttac aggaaagtaa
     6721 actgaagtct ttttttgatt tataatgttt tacactgcaa ttgggtcgtg ataaaaaaag
     6781 aaagtaatta gtaaaaagcc ttttatttta ttttatttta ttttttcttt ctattacaaa
     6841 cctgtcaatc tgacattcat aattttattt taaaaagaca agttgtcttt tctcttattt
     6901 gattgttaaa taatttttat gtttgagaac gctttttaat ttacatacaa ctgaaaatca
     6961 accacttatt tttctgccta atagatttat tcttttgctg gtataggttt tgcaaagtaa
     7021 taagtgaaat caacaaggat ttcaaaaagt aattattaga tgtttaactt aattaaaaaa
     7081 tggaggtgtt taatatggca ttaccaagat tatttacatc acggagcaat tggactcctt
     7141 ctttaccatc gttaattgac agattttttg atagcgattt aatggattgg gatactttca
     7201 actttgccgg tgaaaacatg acattaccag cagtaaacgt aaaagaaaac gacaatgaat
     7261 ttaaaattga agtagctgct cctggtttga acaaagaaga ttttaaagtc aattacgata
     7321 atggtatttt aactatttct tctgaaaaac aagaagaaaa agaagaaaaa gaaggagaaa
     7381 aagtaacacg tcgtgaattt agctatcaat cattccaaag aagcttcagc gtacctgaag
     7441 atatcattaa tgctgataaa atttcagcaa aatatgacaa tggaattttg tatgtaactt
     7501 tacctaaaca cgaagaggca aagaaagcta aaaatgtaaa agaaattaaa atctcataat
     7561 ttcttcttac ttttcaaaag aggaagttca agtaaaaagc ttcctctttt ttatataaac
     7621 tttttgtgaa gggggtacta aaaagaacag ttttaaaaaa taatttctgt ctgatttttt
     7681 ccattaaaat ttcacaattt tcatttcatt tatatttctc gtctattttt ttgtaccatt
     7741 cttgcaggat ataaaaagct tttttcttga tgccattgtt tgaaatcaag ccttttcggt
     7801 tgaaaaaatc ctgaattcca gataattgac ggcgaggtga ataaaaatct accaaaatcc
     7861 agggagaaat tcctgcaagt gcatcgattt tttccagcat ttcgaggttt tttaaataaa
     7921 gctcttcttg atattcttct gtccatcttt cacttttatc tccatataaa ccttgtaaag
     7981 ctcctccacc aaattcacta ataatgacag gtttattata aggaatttcc cattttacca
     8041 aacgacataa atctggtgtg ccattgtacc atcctatata ttcattaaaa ctaataatat
     8101 ctacatattg attcatatta tcttttacag tataaacatt gtcattgctg gaagttactt
     8161 ccatagccat acttattaag cgcgtactat cctgcgattt ggcatatttt gataaactgc
     8221 ttaaaaacaa atctctttcc ttgctttgag gagtttcatt agctattgac caaataatta
     8281 ctgcacaacg atttttatcg cgataaatca tatcatgtaa ttgtcgttca gcatttttat
     8341 aggtctcaga atttccccac gatatagtcc aataaacagg aatttcagac cagaccatca
     8401 atcctgcacg ttcggcttca cgaaccatat tttcgttatg cggataatga gccagacgaa
     8461 cataattaca gcctaactct tttgcccaat caattaatat ttttgcatct tcttttgacc
     8521 aggctcttcc ttgtcgaaaa ggtgcttctt catgaatgct gattcctttc agaaaaactt
     8581 ttttaccgtt tagaaaaatt tcttttcctt tcgtttcaat agtgcgaaat cctatttgat
     8641 cagaaaatgt ttctttatca gtactgaatt ttacttcata tagtttagga gaggtaggtg
     8701 accataattt gggatgtgat tttatttcga agttaactat tccttcctca tttgtagtta
     8761 aagattgttt tattttcaat tcaggaattt caaaaaataa attttggtca cttaaaggtt
     8821 tgtttaattt tatccatcca taaattgttc cgtttttatc tgttttgtac tgaaccgcgt
     8881 aatcttcaat ataaatttct ggtactgtta tcagcataac gtctctggta attcctccat
     8941 aattccacca gtccatgttt aaagtaggaa ctccctcttt ttgtctttta ttatctactt
     9001 tcaatattag aaaattgttt ccttctttta tttcattagt aatatcaaaa ttaaagggag
     9061 taaaaccacc gatatgttct ccaacttttt taccattcag gtagatttta gtatcataat
     9121 ttacagctcc aaaatataag attgttcttg tatcggttga tttatggaaa gtaaagtctt
     9181 ttttaaacca aattgttcct tcataaaaga ataattgagt gttctttgta ttccaatcgg
     9241 aaggaatatc cataagtggt gaagtatcaa aattatattc aacaagttct tgtggatttt
     9301 ttgctttttt attttcaaaa aaaccatttt tttcataggg ttgcattcgg taatcataat
     9361 agccattttc cagtggatca attatataat tccatttacc atcaagactg attacatttc
     9421 gagcataagc atttgccaat ataggtacgt cgttagccat tgaagagaca gggaaaatca
     9481 gaaggaaaag taaaattttg ttgcggatat ttttcttttt catgatgatg gggaataatt
     9541 ttaaattttt gtattaagat aatcaattaa attttcatct ctggaaagtg aaaacttttt
     9601 tctcagtctg taacgtaatg tttcaactcc acgaatagaa atatttagta aaggtgctat
     9661 ttctttggtg gaaagattca tctttaaata aacgcacatc attcgttcat tgaccgaaag
     9721 atcgggatat ttttcttgca gacgcttcat aaagttattg tgaatcaagt cgaattgttc
     9781 ttctatttgt ttcaaaattt cgtctgattt tatattcgaa tcaattttat tattagtcaa
     9841 caacaacatt ctttcaattt ccttgttttc tctgaatttc aaggatgatt taattttaaa
     9901 caaatcagat ttaatttcgg tcaagatttc attttttcgt accagattaa tcatcaaatt
     9961 tgccatttcc tgacttttat attgtaaatc atgctctaat ttttcttttt cgaactgaat
    10021 aatttgctgt tcttttcttt tattttcttc ttcaaattcc ttttccatgt tttttagttc
    10081 ttcatctttt gccaaagtgg cttgaagttt tttctttttt attcgggcat catcccattt
    10141 ataaattcca aacaaacaga atataagtat gataaagtaa aagagataag caattttcga
    10201 acgataccag ggaggcagga taataaaaga aaaattatct gattttattt taccatcttc
    10261 cagcagaatt ctcacttcaa aggtataggt tccttcatgt aatttactgt attccttagt
    10321 gttagagttt gtaaaatctg accaatgctc attattcaag cgatattgat aattaaaatc
    10381 tgtgcgttgt gtaaaagaag aaactccata ttcaattctg atcgtattat gtgaaaatgg
    10441 gatttttggt gttatttttt tatgaagaaa gttatctaaa taaattaacg aatcttttgg
    10501 atgggaaata tagaccttat taatagttag tgaaatacaa ctgttttcat ttgttttata
    10561 atcagagggc actttcagaa aagcgaaacc atattcattg ggaatgacaa atgatgaatc
    10621 attaatagga ataatatttt ctgctccatg cactaaatcc attgtttgaa attcaatggg
    10681 tataatacgt gatttatttc ccgacttaaa aggaaattct gagatgcaga tttccttgtg
    10741 attgatactt attaaatgat ggttgaattc aatcaaacga gaatagtctt tttctccatc
    10801 aagcatttga tttatttcct ttgctggata aatactgtca attttttggt catattgata
    10861 aatgcctttt ggtgtagcga aatatatttc ccctttaatt ttacttatat aaatatttct
    10921 attgcaagga aggccgtttt gggtatcgta ttttttaatg ttaactactt gagttaaact
    10981 gctgtcaagg gtaattttga ttggtttctc tgtattaaag atccataaga tattagatgt
    11041 ttcctcttca aaaaatcggc acgagtcaaa aaaaccttca atttttcgca tcactttcca
    11101 tttattgtct ttcttttcca ataagtataa tccatcataa gttccagcat aaattctgtc
    11161 ttttctccct gatattggtt tacagctcca cacacccgtg atattaccaa ttctatgtaa
    11221 atttgtacct tttatctgaa aaatgcctct atcatggcaa caaaataaat tgttgtctat
    11281 attgcaaaga tcccatactt gtccgctaga agatggtaca ggagaaatat taggttgccc
    11341 ttctaccatt tctactggat atttagtata ataaagtcct ctgttagtgc ctaaatataa
    11401 atagttatct gacaaaagag cagcataacc tgttccatac gaataaggat aagaataaag
    11461 attggtaaat ggtgaattca aacatatgta atcaataccg ttatctaaac ctgcccagag
    11521 attattccat ttatcgaaag ttaaagaaag tactgtgttg ttctgtaatc cattattttc
    11581 gttgaaatat tttactgtat gattttgatt gttgattaac agaatacctc catgaatagt
    11641 gcctaatgct attaattgtt ctgatgaagc tgcacagaat acttcatttt gtttcataaa
    11701 attttcaaaa cctgtgaggt aaggtttaag cgtatgttca tcgtaatagt ataacccatc
    11761 ataagcggtg acaatcaata taccttttcc gtatggcacg acacctcgta tccttttaga
    11821 tattaaagat tctgcacctt gcagagggga aaaagtattg ccaactaaaa cccatacacc
    11881 ttcttctgtg ccaatgtaaa taacgccatt aactaaatcc gagcactcga tttttcgatg
    11941 ggcttctatt ggaaaaaatt ttccattcag atatttaatg attttgttat cgccctggaa
    12001 ataaaggatg ttgtcatttt cataaatttt ccagatattt ccaatttgta aattagcaat
    12061 atcaaatgag tctgacagac agatgtaatt taatttacct ttttcattgg gttcaaaata
    12121 accaagctca ttaatgcttc ctacataaat acgttgttgt ctctttgaag gataaaccga
    12181 acgagcatct gaaccagtat tgagcgggaa taaattccac aaaattccat tgaattcaag
    12241 taaaccattt ttattagcaa aatataccca tcgtgtgtca taaggaacaa tttgccatgt
    12301 ttgagaacct tttccataaa gatttttatc ataattgatt atgaagctgt tccattcggc
    12361 ataaattttt ggacaaaaaa ggaagttcaa agaaatcatc aaaaaaaaga agaaaaaata
    12421 gataaaaatg tttttcatag ttagcttttt taagctaaaa gaacaaattt agcagattat
    12481 ttttataata tcaattttat aaaaaggagt tacaaccttt ttaaaaatag gtgtttttaa
    12541 aaaatttaac cctgtctaaa aaattaacta tttttcagca ttacattcag aaattaattc
    12601 gttgataaag aacaaatata attaaaaatc aggtaagatt taacaatctt tttaaaagta
    12661 atttctttga taatttcgct cactgttaaa tgaggggaac gattttctgt gttcttttat
    12721 catcattttc agttcaatgg ctatacaagc cccataaatt gtttcgctag caagaggctc
    12781 cagcaaaggt acgaaaatga tttccattcc cccgtcaata ttttacaaag tccttcacca
    12841 ccttcatttc cagcgacaaa acgacgatgg aggttgtgct ttcaacttca gcatttcatg
    12901 attagcaacc atactatctc cggcttcact caatctacaa ataggaggga tagcagttcg
    12961 cgagcccgtg caaatcaaca aattggcact ataatatttt ttgccattac aaataatttc
    13021 gataccatta acattctgcc cacgaattat ggcttcactt ttaacaacag taacgttgtt
    13081 tgcctttatc ttattctcca catctaacac caacttgcga accacttatc aacaaaaaag
    13141 aaagtgactt tttatgaact ctctttaata ttttaaattt ctgttaaata tataattttt
    13201 ttagggtatt tatcaaaaca ttattttcgt caggtgttcc tactgtaata cgcaagcagt
    13261 tggtacaaag aggcatgttg tgtcgattcc gtactactat tccttttttg atcaaatact
    13321 gatagatcat gtttgcatct ttgactttta ccagcaaaaa atttgcatcg cttgggaata
    13381 tttcttctac aatggctaat ttacttaatt catttcgtag tttttcacgt tcttccaaca
    13441 gtatgcttac ccattttttc atttgaaaag gatcttgaag gacttttaga gcatgttgct
    13501 gagtaagaaa attaacatta taaggatatt tgattttgtt taatacctga attatttctt
    13561 ctgaggcaaa agccattcct aaccttatgg cagcattgcc ccaggctttt gacatggttt
    13621 gaagaataac aagttgagga tattgagaga tttcttttag gaaactttct ttattagaaa
    13681 aatcaatgta tgcttcatca attaccacta ttccacgaaa ccatttaaga atttttctta
    13741 tttcttcccc atctaaacta ttacctgtag gattattggg agaacatagc cagataattt
    13801 ttgtccgttc attagtagcg tccattaatt tatatgcttc aatgtcaaac tttttattta
    13861 gcagaacttt tcgaaattca acatcattta tctcagcaca aactctatac attccataag
    13921 ttggttcgat agcaactacg ttatcaatac gaggttcaca aaaagcacga taaagcaaat
    13981 caatagcttc atcactaccg tttcctaaaa aaatattgga ggtagatatg ttttttattt
    14041 ttgaaaattg ttccttcaat tctgcctgca agggatcagg ataacgatta taaggatcat
    14101 taaatggatt ttcatttgca tcaagataga tggaagcttc gcctttaaaa tcattccttg
    14161 cacatgaata aggttcaagt ttctttatgt tttctcttaa taatttattg atattcatat
    14221 tttataaatc taatcattaa agcatttttt aaaaattcaa attattttca tctaaacgaa
    14281 cttttacggc attgctgtga gcgtcaagtc cttcattttc agccattaaa ataatagcgt
    14341 tgcttaaatt taccaaacca tctctggtaa gatattgaaa agtgattttt cttaaaaaac
    14401 tatccaggtt tacaccatta taactttttg cataaccatt ggtaggaagt gtatgattgg
    14461 tgcccgaagc ataatcacct gcactttcag gagcataatt gcctaaaaag acagaacctg
    14521 cattaacaat atttaaagat acttcttgtg gaaaacgagt agcaataatt aaatgttcag
    14581 gtgcatattc attaatcata ttaatagcat tttctattga ttttaccaga ataattctgg
    14641 tattttccaa tgattgttgg atgatatctt ttctaagaag ttgttttttt tgccgctcaa
    14701 tttcctctaa aacaggctga attaattttt ctgaagtggt aatcaaaaca cactgactat
    14761 caacactatg ctcagcttgc gacaataaat cggcagcgac aaaaacggga tttgcacttt
    14821 cgtcggcaat aatggcaact tcagagggac ctgcaggcat atcaatagct actccttcca
    14881 ttgaaactaa ctgcttagct gcggtgacat actgattgcc aggtccaaaa attttataaa
    14941 ctttcggcac tgtttctgtt ccataagcca tagctgcaat tgcttgaaca ccaccaatct
    15001 tgaagatccg atgaatgcct gccatttttg ctgcgtacaa aatagaagga tgaattttcc
    15061 catttgatgt tggtggtgaa caaagtatga tttctttaca ttcagctatt ttggctggaa
    15121 tgcccagcat taaaactgtc gagaataaag gagctgtgcc accaggaata tataaaccaa
    15181 ctttttcaat aggtagagct ttttgccagc agttaacgcc gggggaagtt tgtatttctt
    15241 taaaaactgt ctgctgagca gaatgaaact tccagatatt tctgcgtgcc atttcaatgg
    15301 cttcctttaa ttcttttgga accagctttt ctgcttcatt tatttcttct tttgtcactt
    15361 ctaaagtatc gagagaaact ttatcgaaca aagcagtgta ttcttttata gcctgatcgc
    15421 catctttgtg cactttgttt aaaattttct gaacagtttc aaacaaagct cttcgatcaa
    15481 ttactggacg agataatata gatgcccatt tttcttgttc aggatattta aatattttca
    15541 taatctaatt taaaatagat tacaaattaa atactttttt gttagaaatg agcaataaat
    15601 ctaccactat tttattattc tgattttcac attctttttt atcaaagtat tacgcaaatt
    15661 ctgacgaata gctattttta aaaagtttat caagacagga agtgagttca tctataattc
    15721 ttgatcgtac catatcatgc cttagtcaag ttctaccgac atttttacaa cttataattc
    15781 tcagttatac catcaaaatt tttggtcgtg gaagattcaa atcatcactt ataaaaaaca
    15841 aaaaaccttt tgtattttca aatacttaaa agtggatatt tttgtaaaat gcccctaatt
    15901 aacatactat ttgttaaaat caacaaaact accgacatca tctaaattca ttgtaatcct
    15961 tttgagacga atgaaattaa agtttagctg aaatttatca ttaaggataa ttaattcata
    16021 ataaagaaat tccttaaaat tgactatcct caccgcaagc agcgaggtat tacgccaatt
    16081 ttatggcttc gccaccggaa tttctaaaaa aataaaaaca aaagctaccc cgaccaagca
    16141 tcggggaatt cttacgatta a
//
LOCUS       DAOSAS010000028        15341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_2639_length_15341_cov_12.478281, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000028 DAOSAS010000000
VERSION     DAOSAS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15341
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2639_length_15341_cov_12.478281"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PLR72_02300"
     CDS             <1..784
                     /locus_tag="PLR72_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36075.1"
                     /translation="MKPVFKYILLTLGVLCILAYLIMAVWIFSGLRYKEVCHKFEIKF
                     YNNNSPVQLITEEDVAKLIDQKGLTPYGKTLKNIYTDSIENVLRKNPMIKQVECFKTP
                     DGSVYLLVEQRKPKFRLIGYESFYVDEDRNIIPTSVSDAAYLPVVSGRVTKSMAKGEL
                     FDFITFLEKDTFWNAQIEQIYVTNDGKLKLVPRVGTGIIILGDLDDYENKLQKLKKLY
                     VDGFNKIGWNHYKIINLQYKNQIVCTRENQSDRLFLDISEAN"
     gene            805..>1946
                     /gene="ftsA"
                     /locus_tag="PLR72_02305"
     CDS             805..>1946
                     /gene="ftsA"
                     /locus_tag="PLR72_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOK36076.1"
                     /translation="MNGRTYIALNLGSSQISALAAEVLDNGAVRILGSESKISEDVKH
                     GIIEHPSGCAFKVNELLKLLQNSSKIHDIQQVCVGINARSMKVISGLVTRFVGQNKMV
                     TDMLINEMKDECARKYSQNDLIVFQTIPVKYQLDGEYITNPIGKKGVQITGYYNVVIG
                     NKLIKERFDGCFDRTGIMVEYSPLAIESLATVLVEEDERKNGCALIDFGATTTTLGVY
                     KNDILQQLLVVPLGGENITSDIEELGIKKSYAERLKCLKGVSMVKFVESPIYVEMPSE
                     NAEENLRISTSFLANIINARLEEITQPIFKTINQLPFSLEGGIIITGGASKLNYLKEF
                     IEENTGLPVRFGNHSEWLDDKTPEKFFDDTYSQLIGTIVLTDEYRK"
     assembly_gap    1947..2046
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2047..3237
                     /gene="ftsZ"
                     /locus_tag="PLR72_02310"
     CDS             <2047..3237
                     /gene="ftsZ"
                     /locus_tag="PLR72_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOK36077.1"
                     /translation="QGITDVSFVICNTDNQVLIKSPVPIKIQLGVNTTAGLGAGGNPE
                     VARQAAEESIDKIKEVLQDNTKMVFITAGMGGGTGTGASPVIAKVAHDMGILTVGIVT
                     IPFAFEGNLKIKQALEGVAALSEHVDAILVINNEKLKDIYPDLELSNAFAKADDVLTN
                     AAKSIAEIITVPGYINTDFADVYSIMKDGKVAIMNTGYASGENRITKAIENALNSPLL
                     NTNDVNGASKVLLSLYCSTTNQIKMEEVQQIHDFMKKVGRNVQVIWGATFDDTLGDNV
                     KITLIATGYDVSDIPGMPTSIVNKHKNSQFAEKKYSTIDEKKPPIAHSTEEEKELQIQ
                     KAMDEYYGDDISQNTSEKELKVGIEDNIPVVNFKDLEDLENDEILKKVEKIPAWIRKQ
                     KKND"
     gene            3272..3733
                     /locus_tag="PLR72_02315"
     CDS             3272..3733
                     /locus_tag="PLR72_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOK36078.1"
                     /translation="METLFDKISEDIKQAMLARDKVTLEALRGIKKEFLEAKAAKGND
                     GTLSDEAAVKILQKMIKQRKESAQIYAEQNRLELAEAELAEVAVIEKYLPPQMDEEQL
                     KTEINKIIDEVGAKSMQEMGRVIGIAVKKLSGKADSQMIANTIKSLLQSKV"
     gene            complement(3800..5002)
                     /locus_tag="PLR72_02320"
     CDS             complement(3800..5002)
                     /locus_tag="PLR72_02320"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOK36079.1"
                     /translation="MKILVLNCGSSSVKYKLFNLETNEILGSGIVEKIGMKGSCLRHM
                     RHDGQKILLEGEVLEHQTAIEYILGVLTSKRHGTLKSLEEIDAVGHRVVHGGEKFNKS
                     VLITDEVIRKIEECIDIAPLHNPPNLAGIHAIEELLPSVPQVAIFDTAFHQTMPEYAY
                     MYAIPYSLYTKYSIRRYGFHGTSHRYVSKRACDFLGLDYQQTKIITVHLGNGASATAI
                     KNGKSIDTSMGFTPLEGLMMGTRSGDVDLGIATFLMEKEMIGPSTLSTLFNKHSGMLG
                     ISGISSDMRDIKAAIEKGNERARLALEMYEYRIIKYIGAYTAALNGVDLLAFTGGVGE
                     NQASTRTNVCNNLNYLGVKIDEKINMATQGKEALISTPDSSIKVAVIPTDEEFMIASD
                     TYEIVKGL"
     gene            complement(4999..5973)
                     /locus_tag="PLR72_02325"
     CDS             complement(4999..5973)
                     /locus_tag="PLR72_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOK36080.1"
                     /translation="MHEIIVEPIEKYGLSKRSRRRTLFSKIGIVGCGYEGSAIATRAL
                     LNGIEVVFLEPTDEKIANAFSRIEQKLNKKIENWGLTENEKKIVLSRVQGTKNYKDFS
                     DCDFVIEAVRYDDQTGERHVKERKEVFLQLEKVLSPQAIIASNVSTVVITELASELRY
                     KERCVGLHFLSNIPESNIIEIVRGLYTSDETYNKVCQFARLINHQYVAVAESAGLVSI
                     RLFLIELNEACEILMEGISTVEDIDRVLTVGFGHRLGVFRTADQMGIEKIVNLMENLF
                     NEYGNSKYKPSPVLLRLYRAKHFGVRKGKGFYKYDEEGNIIKQGGEKA"
     gene            complement(6021..7016)
                     /gene="pta"
                     /locus_tag="PLR72_02330"
     CDS             complement(6021..7016)
                     /gene="pta"
                     /locus_tag="PLR72_02330"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOK36081.1"
                     /translation="MDLLNKLTERAKANLRKIVLPEGDEIRTLQAADVVLKEKIAQLI
                     LLGKEEKIRKLAEENHLNNIAEAEIIDPENSPKLNEYINLLYEIRKNKGITYEEATRL
                     AKDPLYFGCLMIKNGDADGELAGARNFTTHVLRPAFQIIKTVPGINVVSGLFLMFTPT
                     HYGENGLLIFADCAVNPCPTAEELAQIAMCTAETAHNLAEIEPRVAMLSFSTKGSAQH
                     ELVDKVIKATKIAKEMNPDLLIDGELQADAAIVPSVAERKVPDSPVAGRANVLIFPDL
                     QASNIGYKLVERLSGSQAIGPILQGMAAPVNDLSRGCSVNDIVQMIIITSNLVRK"
     gene            complement(7149..9170)
                     /locus_tag="PLR72_02335"
     CDS             complement(7149..9170)
                     /locus_tag="PLR72_02335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOK36082.1"
                     /translation="MASEKNHLIGKSAIKVENGKMTPEILWSFGRISGVQLSHDKTKI
                     LYHVTYFSIPENKSNTEIFTMNVDGTNKIQITQTSVRENSAKWMKDGKHIAFISSESG
                     SSQLWMMDADGANRQQITQREGGINDFIFSPDESKILFIAEVKFGERTSDKYPDLPKA
                     TGIIVKDLMYKHWDEWIQTIPHPFIADFKNNKIVNEKDILEGEPYECPMKPFGGIEQL
                     NWSPDSKIIAYTCRKKTGKEYTLSTNSDIYFYYVESGKTENKTEGMMGYDMNPKFSPN
                     GKWIAWESMEREGYESDKSRLFVMNLQTGEKKDISSNFDQNCNNLSWSSNSQNIYFVS
                     CWHALTQIYKANIQNKQITPLTNGIHDYEFVIEAKNVLIGVKHSMSKPNEIYSIDPKT
                     GKETELSFENKEILDQLKMGKVEERWITTTDNKQMHTWVIYPPDFDPNKKYPTLLYCE
                     GGPQSPVSQFWSYRWNFQLMAANNYIIVAPNRRGVPGFGKEWLEQISGDYGGQNMQDY
                     LSAIDALAKESFVNKDKLGCVGASYGGFSVYWLAGHHNKRFKAFIAHDGMFNLEQQYL
                     ETDEMWFVNWDFGGPYWDKNNSTAQRTYANSPHLFVDKWDTPILIIHGEKDYRILASQ
                     SMAAFNVAILRDIPAEMLIFPDENHWVLQPQNGILWQRTFFEWLNKWLK"
     gene            9505..10692
                     /locus_tag="PLR72_02340"
     CDS             9505..10692
                     /locus_tag="PLR72_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOK36083.1"
                     /translation="MSLLSKRMGEYKAPQEVQALGVYPYFRPIESEQDTVVKIHGKPV
                     LMFGSNSYLGLTNHPKLKEAAIKAIEKYGTGCAGSRFLNGTLDIHIELEERLAKFVNK
                     DAALVYSTGFSVNAGVIPCLTGRNDYLIFDERDHASIIEGKRLSFSKQFKYRHNDMES
                     LEDILKRCDYDRIKVIVLDGVFSMEGDIAKLPQIVELSKKYNASVYVDEAHTLGVFGK
                     TGAGICEHFGLTDDVDLIMGTFSKSLGTIGGFIASDNNIINYLKHNSRTLIFSASITP
                     ASTACVLAALDVIAEEPWRKEALWANTLRAKEGFLKAGFEIGHTESPIIPLYVRDNIK
                     TFKLTQMLQDEGVFVNPVVSPAVPSEDTLIRYSLMATHTFEQIDESIEKITKVARQLG
                     IIE"
     gene            complement(10814..11761)
                     /gene="corA"
                     /locus_tag="PLR72_02345"
     CDS             complement(10814..11761)
                     /gene="corA"
                     /locus_tag="PLR72_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOK36084.1"
                     /translation="MSELRIFYHEDGKLKISKNLDILKKVDLNNLIWIDLNDVPENVE
                     SELEQFLKIYIQEDEEIEEIEMSSRYIQTDDSIVANSNFLQDNYVMEPVSFIIKNGIL
                     VSVRSSELKSFNETVKKLFVNTSNFPTGYHVLVSIFETRIEYDADMIEEVTDQITNLS
                     KTLNSEDILDQNILILIKDLQEKVMLIRQNIVDKQRVISNMLKCNFFPRDLLSRLNMI
                     IKDINSLFEYTRFDFDRLDYLQDTFLGLANIQQNKIIKIFTVVSVIFLPPTLIASIYG
                     MNFKYIPEFNWKYGYPFSLVLMILVSLIILLYFRKKKWL"
     gene            complement(11853..12632)
                     /gene="rsmA"
                     /locus_tag="PLR72_02350"
     CDS             complement(11853..12632)
                     /gene="rsmA"
                     /locus_tag="PLR72_02350"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOK36085.1"
                     /translation="MAEVKAKKYLGQHFLKDKNVARRIVESLPIQEKTSVLEIGPGMG
                     ILTQFLINQPLIDLVAIEIDHESVIYLHKHFPNLKVIEGDFLQLDLQKIFSGEFYIIG
                     NFPYNISSQIFFKLLENKDIIPSLTGMVQKEVAERLAALPGSKIYGILSVLLQAYYKV
                     EYLFTVDENVFQPPPKVKSAVIQLTRNENLNIGCNEKLFKTVVKTAFNQRRKTLRNSL
                     EPLIEKNHEIYKNSFFDKRPEQLDVTAFIQLTNQIEKAMKQ"
     gene            12826..13326
                     /locus_tag="PLR72_02355"
     CDS             12826..13326
                     /locus_tag="PLR72_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOK36086.1"
                     /translation="MRKIFQRIGFLIGKAIDFFYPPFRKYMTLQFFRYGITGAANMVF
                     DWVLYFIVYNFVLQHRMLHLGFVTMSAHIGALAITFPISFFTGFLLQKYVTFTTSYLR
                     GRVQLFRYLLVVMVNLGLNYVGLKFFVEVVHFYPTPSKMLITIVTTIFSYFAQKKFTF
                     KTLEIS"
     gene            13985..14971
                     /locus_tag="PLR72_02360"
     CDS             13985..14971
                     /locus_tag="PLR72_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOK36087.1"
                     /translation="MSSAFVRKSARFLSWNFLKWLFLLAAIGYLSYQLITFEHYSAML
                     AEFQHFSLYQFLCLVSVLFLLPSNWWLESVKWEMLVEKVQKIDIKIAFKSVLNGILTS
                     FFTPNRVGDFVGRIVYLDKKNKKAGFTLSIINSFTQNFIMIICGMPAFLLFVFRSRNS
                     HSAIFQGFSIKVYLIELLLFLLIGIIFYIFLPFIVRILQKSKVANRFQSFFACLSVYN
                     SWDLLKILMVSLLRYFVFSLQFFLMLRCFGVDLYVLNAMIAIPCTYLFVTFTPSLAFS
                     EVPIRSSYAVIFIGAFSHQLVQIMLATACVWAVNYALPLLGGSFFTLKNEKY"
     gene            complement(14988..>15341)
                     /locus_tag="PLR72_02365"
     CDS             complement(14988..>15341)
                     /locus_tag="PLR72_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HOK36088.1"
                     /translation="LNIDPKGLILKINYKKLKFNDPLETAINVSDFLKNKKKCDVIIC
                     LSHLGCDTIKQKMNDYVIAQNTKYIDVIISGHSHVLLTDAPVKNANEKPVTIAQMGKS
                     GFYLGKIILYLEKKK"
BASE COUNT         4996 a         2383 c         2496 g         5366 t
ORIGIN      
        1 catgaaacct gtctttaagt atatattact cacacttggt gttttatgca tcctggctta
       61 tttgataatg gctgtttgga ttttttctgg tttaagatac aaggaagttt gtcataaatt
      121 cgaaataaaa ttttataata acaattcgcc agttcaattg ataacagaag aagatgtagc
      181 taaactaatt gaccagaagg gtttaacccc ttatggaaaa accttgaaaa atatatatac
      241 agattctatt gaaaatgttt taaggaaaaa tccgatgatc aaacaagttg aatgttttaa
      301 aacgcccgat ggaagtgttt atcttttagt agaacaaaga aaaccaaaat tcagattaat
      361 cggatacgaa agtttttatg tggatgaaga cagaaatata attcctacct ctgtgagtga
      421 tgcagcttac cttcctgtgg tgtcaggaag agtcacaaaa tcaatggcaa aaggggaatt
      481 atttgatttt attacttttt tagaaaaaga tactttttgg aatgctcaaa tagaacaaat
      541 ttatgtaacc aatgatggaa agcttaaatt ggtaccaaga gtgggcacag ggattattat
      601 actcggagat ttggatgatt acgaaaataa gcttcagaaa ttaaaaaaat tatatgtaga
      661 tggtttcaat aaaatagggt ggaatcatta taaaataata aatttgcaat ataaaaatca
      721 aattgtttgc actcgtgaaa atcagtctga tagattattc ttagatatta gtgaagcaaa
      781 ttgaatgatt attcttagaa aattatgaat ggaagaactt atatagcatt gaatttaggt
      841 agttcacaga tttcggcatt agcagcagaa gttttagaca atggtgcagt aagaattctg
      901 ggaagtgaat ctaaaatttc tgaggatgtg aaacatggca tcattgaaca tccttctgga
      961 tgcgctttta aggtcaatga attactaaaa ttattgcaaa acagttcgaa aattcatgat
     1021 atacaacaag tttgtgttgg catcaatgcc agaagcatga aagttatttc tggtcttgta
     1081 actcgtttcg ttggtcagaa taagatggta acagatatgt tgattaatga aatgaaggat
     1141 gaatgtgcaa gaaaatattc tcaaaatgat ttgattgtct ttcaaacaat acctgttaaa
     1201 tatcaattag atggagaata tataaccaat cctataggaa agaaaggagt acaaattaca
     1261 ggttattata atgtagttat tggaaataaa ttgataaaag aacgttttga tggttgtttc
     1321 gatcgaactg gcatcatggt tgaatattca cctttagcaa tagaatcatt ggctactgtt
     1381 ttagtggaag aagacgaacg caaaaatggt tgtgctttga ttgattttgg agcaactact
     1441 accactttgg gagtttataa aaatgatatc ttacaacaat tgttggtagt acctcttggg
     1501 ggagaaaata ttacttccga tattgaagaa ttaggtataa aaaagtcata tgcggaacgc
     1561 ctaaaatgtt tgaaaggtgt gtcgatggta aaatttgtag aaagccctat ttatgtcgag
     1621 atgccttctg aaaatgcgga ggaaaatttg cgaatttcaa cctctttttt agcaaacatt
     1681 atcaatgctc ggctggaaga aattactcaa ccaattttta aaaccatcaa tcaattgcct
     1741 ttctctctgg aaggaggaat tatcattact ggaggagcat caaaattaaa ttatcttaag
     1801 gaatttattg aagaaaatac aggattgcct gttcgttttg ggaatcattc tgaatggtta
     1861 gatgataaaa ccccagagaa attctttgat gatacttatt ctcaattgat cggaacaatc
     1921 gtattaactg atgaatatcg aaaattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnncaag gcattaccga tgtatcattt gtaatttgca acacagataa tcaagtcctt
     2101 ataaaatcac ccgtacctat aaaaatacaa ttaggagtta atactactgc tggacttgga
     2161 gcaggaggaa atcctgaagt tgctcgtcaa gcagcagaag aatctattga taaaatcaaa
     2221 gaagttttgc aagataatac caaaatggta tttattacag caggcatggg tgggggtaca
     2281 ggcactggag cttcgcctgt cattgccaaa gtagcgcatg atatgggaat tcttacagta
     2341 ggaattgtta ccattccttt tgcttttgaa ggaaatttaa aaattaaaca ggcattagaa
     2401 ggtgttgcag ctttgagtga acatgtagat gctattctgg taattaacaa tgaaaaacta
     2461 aaagatattt atcctgatct ggaattatca aatgcttttg ctaaagccga tgatgtgctg
     2521 actaatgcag ctaaaagtat tgctgaaatt attactgttc cgggctatat taacactgat
     2581 tttgctgatg tttatagtat tatgaaagat ggtaaagtag ccattatgaa taccggatat
     2641 gcttcaggcg aaaatcgaat cacaaaagcc atagagaatg ctttaaattc acccttgctc
     2701 aacacaaatg atgtaaatgg tgcttccaag gttttgttga gtctttactg ctcaactacc
     2761 aatcagatta agatggaaga agtgcaacaa attcatgatt ttatgaaaaa agtagggaga
     2821 aatgtacagg taatttgggg tgctactttc gatgacactt taggcgacaa cgttaaaatt
     2881 acgcttattg cgactggtta tgatgttagt gatattccag gtatgcctac ttcaatcgta
     2941 aataaacata aaaacagtca atttgctgaa aaaaaatatt caactattga tgaaaaaaaa
     3001 ccacctattg ctcattctac tgaagaagaa aaggaattac agattcaaaa agcaatggat
     3061 gaatattatg gtgatgatat ttctcaaaat acttctgaaa aagaattaaa agtgggaata
     3121 gaagataata tacctgtagt taattttaaa gacctcgaag atttagagaa tgacgaaatt
     3181 ttgaaaaaag tagaaaaaat tcctgcatgg ataagaaaac agaaaaaaaa tgattaactt
     3241 cttattttgt ttttactaaa aaattatacc tatggaaact ttatttgata aaatttcaga
     3301 agatattaaa caagcaatgc ttgctcgtga taaagtaacc ctggaagctt tacgtggcat
     3361 taagaaagaa tttcttgagg caaaggcagc aaaaggaaat gatgggacat tgtcagatga
     3421 agcagcagta aaaattcttc agaaaatgat caaacaacgg aaagaaagtg ctcaaattta
     3481 tgccgaacag aaccgactcg aattagctga agctgaattg gcagaagtag ctgttatcga
     3541 aaaatattta ccccctcaaa tggatgaaga acagttaaaa actgaaatta ataaaatcat
     3601 tgatgaagta ggagccaaaa gtatgcaaga gatgggaaga gttataggaa ttgcggtcaa
     3661 aaaactttct gggaaagccg atagccaaat gatagctaac actataaaaa gtttgctgca
     3721 aagtaaagtt tgaagataaa aaaggaatta ttcttgactt tcaaagagtg attcctttat
     3781 tttatcgaca atgatttttt tacaatcctt tcactatttc ataagtatca gaagctatca
     3841 taaattcttc gtctgttgga atgacggcaa cctttatgct ggaatcgggt gtacttatca
     3901 aggcttcttt cccttgcgtg gccatattta ttttttcgtc gattttaact cctaaataat
     3961 taagattatt gcacacattt gtacgtgtac tggcttgatt ttcaccaaca ccacctgtaa
     4021 atgccagtaa atcaactcca ttcaaagcag ccgtataagc accaatatat tttatgatgc
     4081 ggtattcata catttccagg gctaagcggg ctctttcatt tcctttttca atagcagctt
     4141 taatatcacg catgtcagat gaaatgcctg aaatacctaa cattccgctg tgcttattaa
     4201 acaaagtaga aagtgtcgag ggaccaatca tctctttttc cattaaaaaa gtagcaattc
     4261 ccaaatcaac atcgcccgaa cgagttccca tcattaaacc ttcaagcggt gtaaatccca
     4321 ttgatgtatc aatagatttt ccatttttga tggcggtagc tgaagctcca tttcctaaat
     4381 gcaccgttat tatttttgtt tgttgataat caagtcctaa aaaatcgcaa gctctttttg
     4441 aaacgtatcg atgactcgtt ccatgaaagc catatcgccg aatgctatat tttgtatata
     4501 aggaataagg aatagcatac atataggcat attcgggcat tgtttgatga aaagcggtat
     4561 caaaaattgc cacctgtgga acagaaggca ataattcttc tatagcgtga ataccagcta
     4621 aattaggtgg attatgtaaa ggtgctatat caatgcattc ttcaattttt cgaattactt
     4681 catccgttat gagtacactt ttgttaaatt tttcaccacc atgaacaacc cgatgaccaa
     4741 cagcatcaat ttcctctaaa gatttaagtg ttccatggcg tttactggta agtactccta
     4801 aaatatattc gattgcagtt tgatgttcca aaacctcacc ttctaaaaga attttttgac
     4861 catcatgccg catatgtctc aagcatgaac ctttcattcc aattttttcc acaataccag
     4921 agcctaaaat ctcattagtt tctaaattga ataatttata ttttactgag gaactaccac
     4981 agtttaatac taatattttc atgctttttc tcctccttgt ttgatgatat ttccttcttc
     5041 atcatattta taaaatcctt ttccttttct tactccaaaa tgtttcgccc tatataaacg
     5101 taataaaaca ggtgaaggtt tatatttgga atttccatat tcgttaaata aattttccat
     5161 taaattgaca attttttcta tccccatttg atcagcagta cgaaaaacac ccagtcgatg
     5221 cccaaatcct actgttaaaa cacgatcaat atcttccacc gttgaaattc cttccattaa
     5281 tatttcacag gcttcattta attcaatgag gaataatctg atacttacta aacctgctga
     5341 ttcagcaact gccacatatt gatggttgat taaacgagca aattgacaaa ctttgttata
     5401 agtttcatct gaagtataaa gtccacgtac aatttctata atgtttgatt ctggaatgtt
     5461 ggataaaaaa tgtaatccta cacatctttc tttataacgt aattcagatg ccaattctgt
     5521 aataacaacg gtagatacat tagaagcaat aatagcttga ggtgataaca ctttttccaa
     5581 ttgcaaaaac acttcttttc tttcctttac atgtctttca ccagtttggt catcatatcg
     5641 aacagcttca attacaaaat cacagtcact aaaatctttg taattttttg taccttgtac
     5701 acgacttaaa actatttttt tttcattttc tgttaatccc caattctcaa tttttttatt
     5761 caatttttgt tcaattcggc tgaaagcgtt ggcaattttt tcatctgtag gttctaaaaa
     5821 tactacttcg attccattaa gaagtgctct tgtagcaata gcactacctt cataaccaca
     5881 accaactatt cctatttttg aaaaaagagt gcgtcgtcgg cttcttttac ttaacccata
     5941 tttttcaatg ggttcgacaa ttatttcatg cataatattt taaaaaatta aaagatcaaa
     6001 atccaattta ataattcaat ttattttctt accaaatttg acgttatgat aatcatttgt
     6061 acaatatcat ttactgaaca tcctcgagat aaatcgttta caggtgcagc cattccttgc
     6121 agaataggac caatagcttg tgatcctgaa agacgttcta ctaatttgta gccaatgtta
     6181 ctggcttgca aatcaggaaa tattaaaaca ttggctcgtc ccgccactgg actatcggga
     6241 acttttctct cagccacaga tggaacaata gctgcatcag cttgtaattc tccatcgata
     6301 agtaaatcag gattcatttc ttttgctatt ttcgttgctt ttattacttt gtcaactaac
     6361 tcatgttgag cacttccttt ggtcgaaaaa ctaagcatgg caacacgtgg ttcaatttct
     6421 gccaggttat gagcagtttc ggctgtacac attgctattt gtgctaattc ttcggctgtg
     6481 ggacatggat ttactgcaca atctgcaaat attaataaac cattttcacc atagtgagta
     6541 ggggtaaaca ttaaaaataa acctgataca acatttatgc caggaacagt ttttataatt
     6601 tgaaatgcag ggcgtaatac atgagtagta aaattgcgtg ctccggctaa ttcgccatct
     6661 gcatctccat tttttatcat caaacaacca aaatataaag gatcttttgc aagtcgtgtg
     6721 gcttcttcat aagttatccc tttatttttt ctaatttcgt aaagaagatt aatatattca
     6781 ttcaattttg gggaattttc aggatcaatt atttctgcct cggctatgtt gtttaaatga
     6841 ttttcttcgg ctaattttct tattttttct tctttcccaa gtaaaatcaa ttgagctatt
     6901 ttttctttta atacaacatc agccgcttgc aacgtgcgaa tttcatctcc ttctggcaaa
     6961 acaatttttc gtaaatttgc ttttgcccgt tccgttaatt tgtttagtaa atccattttc
     7021 atttttatat ggaagtaagc actttgaatg ttgaagaaca cttacaaaat taataaaaaa
     7081 aatgaaattg caatgtcgag atgacaattt ttaataaaaa tttttcttga aaataaaatt
     7141 attagcaatt attttagcca tttattcaac cattcaaaaa aggttcgttg ccataggata
     7201 ccattttgcg gttgcaatac ccaatggttt tcatctggaa aaatcaacat ttcagccgga
     7261 atatctctta atattgctac attaaaagca gccatagact gcgaagcaag gattctgtaa
     7321 tctttttctc catgaatgat taaaataggt gtatcccatt tgtctacaaa cagatgtggt
     7381 gaattagcat aagttcgttg ggcagtagaa ttattcttat cccagtaagg accaccaaaa
     7441 tcccagttta caaaccacat ttcatccgtt tcaaggtatt gttgttccaa gttaaacatc
     7501 ccatcgtgag cgataaaagc tttaaatcgt ttgttgtgat ggcctgccag ccagtaaacg
     7561 gaaaatccgc cataactggc tcccacacat cccaacttgt ctttgttgac aaaagattct
     7621 tttgctaatg catcgatagc tgaaagataa tcttgcatgt tttgtccgcc ataatcgccg
     7681 ctgatttgtt ccagccattc tttgccaaaa cctggaacac ctcttcgatt aggagcgaca
     7741 ataatataat tattggcagc cattaattga aaattccatc gatatgacca aaactgactg
     7801 actggcgatt gtggaccacc ttcacagtag agcaatgttg gatatttttt gtttggatcg
     7861 aaatcaggtg gataaataac ccacgtatgc atttgcttgt tgtccgtcgt tgtaatccat
     7921 cgttcctcaa cctttcccat ttttaattga tccagtattt ctttattttc aaaagaaagc
     7981 tctgtttctt ttcctgtttt tggatcaatg ctataaattt cattgggttt actcatggaa
     8041 tgttttacgc caataagaac gtttttagct tcgataacga attcataatc gtgaattcca
     8101 ttagtaagag gtgtaatttg tttattttga atgtttgctt tgtaaatttg agttaaagca
     8161 tgccagcaac ttacaaaata gatattttga ctattggaac tccaggataa attattgcaa
     8221 ttttgatcga aattgctgga aatatctttc ttttctcccg tctgtaaatt cattacaaac
     8281 aaacgacttt tatccgactc gtaaccttca cgttccatgc tttcccaagc tatccatttg
     8341 ccgttgggtg aaaatttagg attcatatca tatcccatca ttccttctgt tttattttca
     8401 gtctttccac tttccacata atagaaatag atatcagaat tagtggaaag cgtatattct
     8461 ttacctgttt ttttccgaca agtataggca atgattttac tatccggact ccaattgagt
     8521 tgctcaatcc caccaaaagg tttcatggga cattcataag gttctccttc cagaatatct
     8581 ttctcgttta cgattttatt gtttttgaaa tcagcaataa aaggatgtgg aatagtttgt
     8641 atccattcgt cccagtgttt atacatcaag tcttttacaa taatgcctgt ggctttcggc
     8701 aaatcagggt atttgtcgct ggtacgttca ccaaatttta cctcagcaat aaaaagaatt
     8761 tttgattcgt cgggtgaaaa aataaaatcg tttataccac cttctcgttg agtaatttgt
     8821 tgacgattag caccatcggc atccatcatc cacaactgtg aacttccgct ttctgaagaa
     8881 ataaaagcta tatgtttccc atctttcatc catttagcac tattttcacg aacagaagtt
     8941 tgggtaattt gtattttatt cgttccatcg acattcatag tgaaaatctc ggtgttgctt
     9001 ttgttttcag gaatgctaaa atatgtaaca tgataaagaa tttttgtctt atcatgagaa
     9061 agttgaacgc ccgatattct tccaaacgac caaagaattt caggcgtcat tttcccattt
     9121 tcaactttaa tagcagattt accaattaga tgattttttt ctgaagccat acaaattgtt
     9181 gataaagtga ccattgttaa aataaataat ttaaatattt gatgcatatt ttgttggaat
     9241 ataaacttag gttatattta tctttataag aataacaaaa gtaagatttt agactgttat
     9301 attcaagcaa tgttttttta ttgaaataat tagtagaaat gaaagcgtta gtaatcaata
     9361 ggataaatgt ttaatatctc cttaaaaaac ttgctttttt tgtagtagaa gatagaaatt
     9421 gtatttaatt tttttcaaca atccattatt ttttaaaaat gatgtatctt tgtgacgttt
     9481 tttaataaat aaaaacaaaa taatatgagt ttattaagta aaagaatggg ggaatataaa
     9541 gcccctcaag aagtgcaagc tttaggagta tatccttatt ttcgtcctat tgaatctgaa
     9601 caggatactg tagtcaaaat tcatggaaaa cctgttttaa tgtttggatc gaatagttat
     9661 ttaggtttaa ctaatcatcc taaattaaaa gaagcagcta tcaaagccat tgaaaaatat
     9721 gggacaggtt gtgcaggttc tcgttttctg aatgggacac tggatattca catcgaatta
     9781 gaagaaaggt tagcaaagtt tgtaaataaa gatgcggctc tggtttattc tacaggattt
     9841 tccgtgaatg ccggtgttat tccttgctta acaggcagaa atgattattt gatttttgat
     9901 gagagagatc atgcttccat tattgaagga aaaaggttat ctttttcaaa acagtttaag
     9961 tatcgtcaca atgatatgga gtcgcttgaa gatattctaa aacgttgtga ttatgataga
    10021 ataaaagtga ttgttttgga tggtgttttc agtatggaag gagatatagc caaattgcct
    10081 caaattgttg aattatcaaa aaaatacaat gcctctgttt atgtggacga agctcatacg
    10141 ttaggtgttt ttggtaaaac aggagctgga atttgtgaac atttcggctt aactgatgat
    10201 gttgatttaa ttatgggtac ttttagcaag tctttaggaa caattggtgg ttttatagct
    10261 tctgacaata atattattaa ttatttaaag cataattctc gtactttaat ttttagtgca
    10321 tccattacgc ctgcttcaac tgcgtgcgta ctggcagctc ttgatgttat tgcagaggag
    10381 ccatggagaa aggaagcttt gtgggcaaat accttgcgtg ctaaggaagg gtttttaaag
    10441 gctggatttg aaattggtca tactgaatct cctattattc ctctttatgt acgtgataac
    10501 ataaaaactt tcaagctgac gcaaatgtta caggatgaag gcgtttttgt caatcccgtt
    10561 gtttctccag ccgtgccctc tgaagatacg ctgattcgtt attcattaat ggctacacat
    10621 acttttgagc aaattgatga atccattgaa aaaattacta aagttgcccg tcaacttgga
    10681 ataatagaat aaacccgttg tttatataaa tgtaaaaagt agagcaagag aatttgttct
    10741 acttttctgt ttataatatc tgaatttttt taaaaatatt cctgattttg aagataatct
    10801 taaatagatt tttctataac catttctttt ttctaaaata aagcaatatt ataagggaaa
    10861 ctaaaatcat taataccaat gaaaatggat acccatattt ccaattaaat tctggaatat
    10921 atttaaagtt catcccataa atacttgcaa taagtgtggg aggcaaaaat ataacagata
    10981 caacagtaaa tattttaata attttgtttt gctgaatatt tgctaatcca agaaaagtat
    11041 cctgcaaata atccaatcga tcaaaatcga atcgtgtata ttcaaataaa gaattaatat
    11101 cttttatgat catattcaaa cgagataaaa gatcacgagg aaagaaatta cattttaaca
    11161 tattagaaat gactcgttgt ttatctacaa tattctgccg gataagcatc actttttcct
    11221 gtaaatcttt gataagtatt aaaatatttt gatccaggat atcttcacta tttaaagttt
    11281 tacttaagtt ggtaatttgg tcagtcactt cttcaatcat gtctgcatca tattctatac
    11341 gggtttcaaa gatcgaaacc aaaacatgat aacctgtggg aaaattactg gtattaacaa
    11401 aaagcttttt tacagtctca ttgaatgatt ttaattctga actacgtacc gaaaccaaaa
    11461 ttccattttt tataataaat gaaactggct ccattacata attatcttgt aagaaatttg
    11521 agtttgccac aatactgtca tcagtttgga tataccggga actcatttca atttcttcaa
    11581 tttcttcatc ttcctgaatg taaattttta aaaattgttc taattcggat tctacatttt
    11641 ctggaacatc gttcaaatca atccagatta aattattaag atctactttt ttgaggatat
    11701 caagattttt tgaaattttt aatttaccat cttcatgata aaatattctt aattctgaca
    11761 tgattgcctc ctttctattt tttattactg aaggattatt aaattatcca gcagtaagta
    11821 gtgtaacaac aaaatgaaaa attattattt ggttattgtt tcattgcctt ttctatttga
    11881 ttggtaagct gaataaaagc agttacatcc aattgttcag gacgtttatc aaaaaatgaa
    11941 tttttatata tttcatgatt tttttctatc aaaggttcca atgaatttcg caatgttttc
    12001 cgtcgttggt taaaagctgt ttttacaact gttttaaaaa gtttttcgtt gcaaccgatg
    12061 ttcaaatttt catttcgagt aagttgaata acagctgatt tcacttttgg cggaggttgg
    12121 aagacatttt catcaacagt aaataaatat tctactttat aatatgcttg aagcaacaca
    12181 ctcaaaatac cgtaaatttt gctgcctggt aaagcagcca accgttcagc tacttctttt
    12241 tgcaccattc ccgttaggga tggaataata tccttatttt caagaagttt aaaaaatatt
    12301 tggcttgaaa tgttgtaagg gaaattgcca ataatataaa attcaccaga aaaaatcttt
    12361 tgtaagtcta attgaagaaa atcgccttca ataactttta aattagggaa atgtttatgg
    12421 agataaatta ctgattcgtg atcaatttca atagcgacaa gatcaataag aggctgattg
    12481 ataaggaatt gagttaatat acccatccca ggcccaattt ccagtactga agttttctcc
    12541 tggatgggta aactttctac aattcggcga gccacatttt tgtccttcaa aaaatgttgc
    12601 cctaagtatt tttttgcttt tacttcggcc attactgtga taatcgtcgg acatgataaa
    12661 caacccaata aaattaatta aaaaatgaaa aattaagatt aacaaacaaa attgtatctt
    12721 tgtaacctcg aaattttcta ctgcaaaagt aattaaaatt gttagaaact tcttcttttt
    12781 taaaaaatta ttaatacgat tttagatttt tatgtcttat aatgaatgag gaaaattttt
    12841 caaaggattg gttttttaat aggtaaagca attgactttt tttatccgcc ttttcgtaaa
    12901 tacatgactt tgcaattttt cagatatgga atcacaggtg ctgcaaatat ggttttcgac
    12961 tgggttttat attttattgt gtataatttt gttttacaac acagaatgct tcatctgggt
    13021 tttgtaacca tgagtgctca cattggagca ttggcaataa ctttcccaat ttcttttttt
    13081 actggttttt tactacaaaa atatgttact tttacaactt cttatttacg agggcgagta
    13141 caactgttta ggtatttatt agtggtaatg gttaatttgg gtttgaatta tgtgggctta
    13201 aaatttttcg tcgaagtagt ccatttttat ccaactcctt ccaaaatgtt aataactatt
    13261 gtcacaacta tttttagcta ttttgctcag aaaaagttta catttaaaac actcgaaatt
    13321 tcttaatgca aatttttttt ataaaatttg aaattgattg tacaaatatt caatggtgaa
    13381 aaaagtatat aattcaagat aaaaggaaaa acttttaccg aagtagaaga tgaaatttta
    13441 cgattaatca actaaataag gtaataaggt tataatcaag gaaataattc ggctttatta
    13501 aggtcaattg tttttaataa ggttttatta aaattaaaca atttgccgat gtgagatgat
    13561 tttggcgggt aaaatagaat ttttatctct gtaaaactct atgaaaatga acggttatta
    13621 cactataacg taatggtcaa aatgctaatt cagtagagac gccatatatg gcgtattcta
    13681 ctgaaatgcg ttgaataaca gttgattatt cataaaaata atgcttgaaa ttttctagtt
    13741 caataaaatg tggttaattt ttcaacctta ttatgttaaa aaccttcata ataagaaaaa
    13801 aacaaataaa tcaaacctta ttaccttatt gtctcaattt taaaaaatta ttgcacaaat
    13861 gttttagagt tttttcaata gaattataaa tatcaaatat ttatcaattt tcatttcaaa
    13921 gaatgatttg tgttatcata aaattaaagt ctatataaaa ttattttaaa tcttacgtga
    13981 aaaaatgtca tctgcttttg ttcgaaaatc agcaagattt ttaagttgga attttcttaa
    14041 atggttattt ctactggctg ctattggcta tttaagttat cagttgataa cttttgagca
    14101 ttattcagca atgttggcag agtttcagca tttttctttg tatcaatttt tgtgtttggt
    14161 tagtgttttg tttttgcttc caagtaactg gtggttagaa tccgtgaaat gggaaatgtt
    14221 ggtggagaaa gttcagaaaa tcgatattaa aattgctttt aaatcagttt taaatggtat
    14281 tttaacttcg ttttttacgc caaatcgggt gggcgacttt gtaggacgaa ttgtttatct
    14341 ggataaaaag aacaaaaaag ctggatttac tttgagtatc attaatagtt ttacccagaa
    14401 ctttattatg atcatatgtg gtatgccggc ttttttattg tttgttttta gatcaagaaa
    14461 ttcacattct gctatttttc agggcttttc gataaaagtt tatttgatag aattactatt
    14521 atttttacta attggtataa tattctatat ttttctacct ttcattgttc gtattcttca
    14581 aaaatctaag gtcgcaaatc gtttccaaag tttctttgct tgtttatcag tgtacaattc
    14641 gtgggattta ctgaaaatat tgatggtctc tttgctacgt tattttgttt tttcgttaca
    14701 attttttctt atgcttcgtt gttttggtgt tgatttgtat gttttgaatg ctatgattgc
    14761 cattccttgt acttatcttt ttgtcacttt tacaccatcg cttgcttttt cagaagttcc
    14821 tattcgaagt tcttatgctg tgatttttat aggagctttt tctcatcaat tggttcaaat
    14881 tatgttagca actgcttgcg tatgggctgt taattatgct cttccacttt tgggaggttc
    14941 attttttact ttgaaaaatg agaaatatta atcagatttt tcaagtatta ttttttcttt
    15001 tccagataca aaataatttt tcccagatag aagccgcttt tacccatttg agcaattgtt
    15061 acaggttttt cgttagcgtt ttttacagga gcatcggtta aaagcacatg agaatgtcca
    15121 ctaataataa catcaatata ctttgtattt tgagcaatca cataatcgtt cattttttgc
    15181 ttaatggtat cacaacccag atgtgaaagg caaataatta catcacattt tttcttgttt
    15241 ttcaaaaagt cggaaacatt tatagctgtc tcaagcgggt cgttgaattt caatttttta
    15301 taattaattt ttaaaatcaa tcctttgggg tcaatattca g
//
LOCUS       DAOSAS010000029        13971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_2957_length_13971_cov_10.990874, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000029 DAOSAS010000000
VERSION     DAOSAS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13971
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2957_length_13971_cov_10.990874"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLR72_02370"
     CDS             <1..586
                     /locus_tag="PLR72_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK36089.1"
                     /translation="KKNGSYSFTQRLKLGGSGIPDIQPGDNRYMPTTRALVFQVTGPG
                     DISVCCLSSSSSENRRLLLTNGTDSISSFDAPGTYSDDNKNTVPYETFHYTGGPATLY
                     LYSVRSGVNIYLLDVTSAIDIVDFINTPKKGTFVSYNGLEIINNNGLSLEVYNITGKK
                     IISSSEAKIATSQLPEGIYIVREKGTANTLKFIK"
     gene            1239..3749
                     /locus_tag="PLR72_02375"
     CDS             1239..3749
                     /locus_tag="PLR72_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK36090.1"
                     /translation="MKKTVFLFLNFFIFISFSAFSQSIEIFETNGWLESAYIKWSASG
                     SVNSYNVYYSGEGITNKKIDSQLIRKYVDYWRADIPGLKEGTYSFMVKPVINGVESEG
                     AIVNSITVKSHDRSGFAFKNGRVPGAYKSDGTLKDNAVILYITESNKNSVSMTVTGAS
                     SNPCIGLQAILDGFKKGKDTRPLSVRLIGQITDLNYMLNGDIVIENNNNASSYITVEG
                     IGEDAVADGWGLRIKNASNIEVRNLGFMNCDSDEGDNVSLQQNNDYIWVHNCDFFYGE
                     AGSDADQIKGDGALDCKKSTYVTFSYNHFWDCGKCNLLGLGEGTTSGLYATYHHNWYD
                     HSDSRHPRVRYYSTHVYNNYYDGNAKYGVGSTTGASVFVEGNYFRNCKYPMLISKQGT
                     DIYQSSGTFSGEDGGIIKAYNNYMTGQNRFVAYGSSDFANSTVDFDAYVAASRSETVP
                     STVKTKQGSNTYNNFDTDASVMYQYTADTPEQAKEKVMQYAGRVNGGDLKWTFNNAID
                     DASYDINTGLKSALTNYSTSLVAVQGESSSSSSSQTLVVTGEANQTVILGNAISNIVF
                     TWGGDATDASVTGLPASGIDYVKDASTKTITIYGTPTATVSYTVTTSGTLGSPISISG
                     IITVTNGSGSTGAMVHNFTTDGKNSTFYSITGNLSTNYGTVIYQGLTLTQCLKMESST
                     NISFTTTSEATLTLVFNPDYTGTIKIDGEIYNPSLSGGIVTITTLPAGTHTILKGSGS
                     SYLFYISTVYNPISIVEKIHDQKLSVELYPNPVSTRLNINSNNKVTKVEIYNVSGLLV
                     KSLNMPGSQIDLSNLKSGIYIVKIFTENNLIKKTIIKN"
     gene            4348..5160
                     /locus_tag="PLR72_02380"
     CDS             4348..5160
                     /locus_tag="PLR72_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOK36091.1"
                     /translation="MYELFNIQNKVIVITGGTGVLGSSMVEYLAAHGAKMAVLARNKQ
                     KGDELINRVKSNGGEAIFLQTDVTNEAILTQNAEDIVAKYGRIDVLINAAGGNMPGAI
                     ISPDGTIFDLKMDDFRKVVDLNLMGTVIPTIIFAKIMVANKQGNIINISSEAALRPLT
                     RVVGYAAAKAAVTNFTKYMAGELAIKFGENFRVNAIAPGFFITEQNRTLLTNPDGSYS
                     DRGKAIIQHTPFRRFGVPEDLHGTLHYLVSDASKFVTGTVAVVDGGFDAFCI"
     gene            5173..6252
                     /locus_tag="PLR72_02385"
     CDS             5173..6252
                     /locus_tag="PLR72_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOK36092.1"
                     /translation="MSLLLKKDCKYSLLVPTSMGVRITPVNAQPVHCSTLFEMQATSA
                     ETNVASISSYLGLPVKVLTTFVKDSPIAKFIKADLRRRNMDFEGPEVPQGGPWGYRHQ
                     FNIADSGFGLRGPRVHNDRAGEVGRTLHVKDFDLERIFAQEGVQIVHLSGLIAALSPD
                     TGNFCLEIARFAKKHGTLISFDLNYRASFWKGRETELSAIFKEIASLSDILIGNEEDF
                     QLCLDIKGPQPGGEDIDAQLDAFKEMIKNAHKTYPNASVFANTLREVVSANEHLWGAI
                     VYENDKWHIIRPRKINVLDRIGGGDGFVGGLLYSILKEWPAEQWIQFAWACGALATTF
                     LTDYAQPADEEMIWNIWEGNARVKR"
     gene            6275..7729
                     /gene="gnd"
                     /locus_tag="PLR72_02390"
     CDS             6275..7729
                     /gene="gnd"
                     /locus_tag="PLR72_02390"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOK36093.1"
                     /translation="MKKLADIGLIGLAVMGENLVLNMESKGFTVAVFNRTVSKVDEFI
                     NGRAKGKNIIGTHSLEELVASLSRPRKVMLMVKAGQPVDDFIEKLIPLLEPGDIIIDG
                     GNSYFMDTRRRTNYVESKGLLYIGTGVSGGEEGALRGPSMMPGGSSAAWPHVKDIFQA
                     VAAKVDDGTPCCDWVGEDGAGHFVKMVHNGIEYGDMQIINEAYHLMKELLGLTTDEQH
                     EVFKKWNTGKLNSYLIEITADILAFKDSDGTPLVEKILDTAGQKGTGKWTVNTALDLG
                     IPLTLIGESVFARCLSAQKDLRVKASKTISGTKPHFKGDKQRFINDLEEALYAAKIIS
                     YAQGYDLMREAAKEYNWNLNYGGIALMWRGGCIIRSAFLGDIKKAFDRNPSLENLLLD
                     PFFKDAVESAQESWRSVCTAALENGIPIPALASALCYFDGFRCERLPANLLQAQRDYF
                     GAHQYERIDRPRGEFFHTNWTGHGGDTASSTYQV"
     gene            7793..9079
                     /locus_tag="PLR72_02395"
     CDS             7793..9079
                     /locus_tag="PLR72_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOK36094.1"
                     /translation="MIYYQNGSSQHNLSHEDLKKSLIAALDKLGRKHKILAIPPDYTR
                     LPSRAGELTEMVWEYYGNTLTDILPALGTHTPMTDEQISHMFGKTPRNLFRVHDWRHD
                     VITLGEVPAEYVKEVSEGKVDFSWPAQVNKLLVEGNFDLILSIGQVVPHEVVGMANYN
                     KNIFVGTGGIEGINKSHFVGAVYGMERMMGRADTPVRKLFNYASKNFANQLPIVYILT
                     VVGINKEGKQQTYGLFVGDDVDVFDEAAKLSLEVNFEMVEEPLKKILVWLDPTEFKST
                     WLGNKSIYRTRMAIADGGELIVLAPALKEFGEDKEIDRLIRKYGYHGTSATLKAIAEN
                     EELRNNLSAAAHLIHGSSEGRFTITYCPGKNPENLTKEEIESVGFQWADIDEVMNRYH
                     FRELKEGYNILPDGEEIFFISNPALGLWAHKDRFEF"
     gene            9657..10172
                     /locus_tag="PLR72_02400"
     CDS             9657..10172
                     /locus_tag="PLR72_02400"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HOK36095.1"
                     /translation="MSYLQKYSLRICWMLILWSIGNSCSTTDEFCREEKNVKLKMSFY
                     TRNFNSNTQKYVNSSLSVDSLTIVGIDSLGQLIDSVLYNNSKNISSVKLPLNKFSKES
                     KFLLILNDELDTITIHYTNHLQYLSFECGSIITHTIDTVYATSHFIDSLTIVEPDITT
                     LDVENLQIFHY"
     gene            10141..10824
                     /locus_tag="PLR72_02405"
     CDS             10141..10824
                     /locus_tag="PLR72_02405"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HOK36096.1"
                     /translation="MLKICKYFIISFLIFFFSHINVFSQNKKENLPKIKWWNGIMLNF
                     DVVSPIQSSLSSSQNFSAVGNVQINLKDIFLPVYEIGIDGINKTSSQEIYYKANGIFQ
                     RAGIDFNLLRQKKEAEPSNNKLLGGIRVGWSNFKYDVTNITITDDYWGGESILNYERQ
                     SASKVWYEIVFGIQVEVFKNVYLGWNIRSKNLFKQNTSTEVSPWYIPGFGKNNTSNVG
                     FNYTVGYHF"
     gene            10873..12897
                     /locus_tag="PLR72_02410"
     CDS             10873..12897
                     /locus_tag="PLR72_02410"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOK36097.1"
                     /translation="MNSIETLNRAADNIRILAAAMVEKAKSGHPGGAMGGADFMNVLY
                     SEFLEYDPENPFWEGRDRFFLDPGHMSTMLYAVLAFSGKFTLDELKQFRQWGSPTPGH
                     PELDIKRGIENTSGPLGQGHTYAVGAAIAAKFLKARFGEVMDHTIYTFISDGGIEEEI
                     SQGAGRIAGHLGLDNLIMFYDSNNIQLSTETKVVTSENTAQKYEAWGWKVITIDGNDA
                     AQIRKALTEAKAEKERPTLIIGKTIMGKGIKKADGSSFENQISTHGMPISEAGGSFEE
                     TIKHLGGDPANPFVIFEETKTLYAKRAEELKEIVSKKQAIKNSWAKENPQLAEKLKFF
                     FSSEIPPVKWDAIVQTPNKSTRAASSVVLAELAKQVENMIVASADLSNSDKTDGFLKQ
                     TKAFAKNDFSGAFLQVGVSELTMAALCVGMSLHGGVIPACGTFFVFSDYMKPVVRMAA
                     LMEQPVKFIWTHDAFRVGEDGPTHQPVEQEAQIRLMEKLKNHSGNNSMLVLRPADAEE
                     TTVVWKLALENTKCPTALIFSRQDIVSLPAIENRRKEAEQASRGAYVVQCDGNPEIIL
                     VASGSEVSTLAEAAVLLRKDGIKVRLVSVPSEGLFRLQPITYQERVLPPNSKIFGLTA
                     GLPVTLQGLVGNNGKIFGMESFGFSAPYKVLDEKLGFTPQNIYEQIKKML"
     gene            12963..13535
                     /locus_tag="PLR72_02415"
     CDS             12963..13535
                     /locus_tag="PLR72_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOK36098.1"
                     /translation="MDIVSIILIAFGLAMDCFAVSISKGIAVKKFYFSSTLLMAFLFG
                     FFQAAMPLIGYFAGLKFITVIKDSDHWLAFVLLALIGGKMLLEGIKKHSSEKDDGEAH
                     PFYWMKLLTLSLATSIDALATGIIFVPYPLMVWKAITIIGTISFLMSILGMYIGVRFG
                     KRFHVNVEILGGIILIGIGFKILLQHTVLV"
     gene            complement(13532..13897)
                     /locus_tag="PLR72_02420"
     CDS             complement(13532..13897)
                     /locus_tag="PLR72_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOK36099.1"
                     /translation="MSFHNEVGATGEKRAQEYLISKGYKIRHTNWHVQKYELDIVAEK
                     DGLLVIVEVKTRSTENFEHPQEAITKKKIRNIINATQEYILKFDWKGETRFDVISVIP
                     QKNGFRIEHIEDAFLSPIS"
BASE COUNT         4638 a         2314 c         2712 g         4307 t
ORIGIN      
        1 aaaaaagaat ggttcctatt cttttactca gcgtttgaaa ttaggtggat caggtatacc
       61 tgacatacaa cctggagata atcggtatat gcctaccacc cgtgcgctgg tatttcaagt
      121 aacaggtcct ggcgatatat cggtatgttg tctctcgtca agtagtagtg aaaatagaag
      181 attattgctt actaatggaa ctgattcaat ttcttcattt gatgctcctg gtacttacag
      241 cgatgataac aaaaacactg ttccgtatga aacttttcat tatactggtg gacctgctac
      301 attgtattta tattcagtta gaagtggtgt aaatatttat ttattggatg taacttcagc
      361 aattgatatt gttgatttta taaatactcc taaaaaaggc acttttgttt cttataatgg
      421 cttagaaatt atcaataata atgggttatc tcttgaagtt tataatataa ctggaaaaaa
      481 aattatcagc tcatcagaag caaaaatagc tacttctcaa cttcccgaag ggatatatat
      541 tgttcgcgaa aaaggtacag caaatacttt aaaattcatt aaataattat aacttaatga
      601 attgaaatta atagtattta agaagccgtc ttaaaaatgt aagacggctt ttaattaagt
      661 acctatttaa caaattaata ttttgaatac aaaaccataa tattttagta tcacaaacac
      721 aaataaaaat atttattatg gcagcattat gaggtattgg attttttgct taaaagttta
      781 aggaaaagat ggtatgtttt tgaaatctat tttgttgctt gtagaggaat aaaattcatt
      841 aatttgaaca agaagaaaag tagattttac attgttgaat aaatacgaca caagttaata
      901 aaagaacttt attattctta aaaccttgat gacttattgg caaacaaatc agtcaaacct
      961 tattacctta ttaaaaaacg agtgaaaatg taaattggag aggaatatca atttacctaa
     1021 ataaattaat aaggttataa aaaaagaaaa tttcaatgtt atgaaggttg aaaaattgat
     1081 aaggttttaa aggcaaaagt tgacgaaatt taaagcaata aaactgactt caaacagatc
     1141 agcataaacc caggtatttt gagtgggaaa gcgaattttt aattttttat ctttttttaa
     1201 aatactaata ctgtttaatt taataaaatt attttcaaat gaaaaaaact gtttttctct
     1261 tcttaaactt ctttattttc atttcatttt cagcattttc tcaatcaatt gagatttttg
     1321 aaacaaatgg ttggctggag tcagcgtaca tcaaatggag tgcttctggg tctgttaata
     1381 gttacaatgt atattactca ggggaaggta ttacgaataa aaaaattgac agtcaattaa
     1441 ttagaaaata tgtggattat tggagagctg atattcctgg attaaaagag gggacctaca
     1501 gttttatggt aaaacctgtc atcaatggtg ttgaaagtga aggagccatt gttaattcta
     1561 taacagtgaa atcacacgac agaagcggtt ttgctttcaa aaatggtcgt gtaccaggtg
     1621 catacaaaag cgatggaact ttgaaagata acgcagtaat tctctatatt accgaaagta
     1681 ataaaaattc ggtatccatg actgtaactg gtgccagctc caatccttgc attggtttac
     1741 aagccattct ggatggtttc aaaaaaggga aagatacgcg tcctttatcg gttcgattga
     1801 ttggacaaat taccgacctt aattatatgt tgaatggtga tattgtaatc gagaacaaca
     1861 ataatgcttc aagttatatc actgttgaag gcatcggaga agatgcagta gctgatggtt
     1921 ggggattacg tataaaaaat gcttctaaca tcgaagtcag aaatcttggt tttatgaatt
     1981 gcgacagcga tgaaggagat aatgtcagct tacaacaaaa taatgattat atctgggtgc
     2041 ataattgcga ctttttctat ggtgaagccg gcagcgatgc cgatcaaatt aaaggagacg
     2101 gagcacttga ctgtaaaaaa tctacttatg ttactttttc ttacaaccat ttttgggatt
     2161 gtggaaaatg caatttgtta ggtctgggag aaggaaccac ttcaggactt tatgcaactt
     2221 atcatcacaa ttggtacgat cattcagatt ctcgccatcc tcgcgttcgc tattattcta
     2281 cccatgttta taataattat tacgatggaa atgcaaaata cggtgttggc tcaaccacag
     2341 gtgcctctgt atttgtagaa ggaaattatt tccgcaattg caaatatccg atgctgattt
     2401 ccaagcaagg tactgatatc tatcagtcaa gtggtacttt ttcgggtgaa gacggtggca
     2461 ttatcaaggc atacaataac tacatgaccg gtcaaaatcg ttttgttgct tatggaagct
     2521 ccgattttgc taattcaact gtcgatttcg atgcatatgt ggctgcctca agaagtgaaa
     2581 ctgttccttc gacagtgaaa acaaaacaag gttcaaatac ctataataat tttgatacag
     2641 atgcgtccgt catgtaccaa tatacagcag ataccccaga acaagcaaaa gaaaaagtaa
     2701 tgcaatacgc cggacgagtt aatggtggcg atttgaaatg gacatttaat aatgctatag
     2761 atgacgcatc atatgatata aataccgggt tgaaatcggc tcttacaaat tatagtacaa
     2821 gtttagtagc tgttcagggt gaaagtagtt cttcaagtag ttcacaaaca cttgttgtaa
     2881 caggtgaggc caatcaaacc gtaattttag ggaatgccat tagtaatatt gttttcactt
     2941 ggggtggcga cgcaactgat gcttcagtta caggattacc tgcttcagga attgattatg
     3001 taaaagatgc ttctaccaaa acaatcacta tttatggcac accaacagca acagtttcct
     3061 atactgttac aacatccgga actttaggaa gtcctattag tatttctggt attattactg
     3121 ttaccaatgg ttctggttca actggtgcaa tggttcataa ttttacaacc gatgggaaaa
     3181 acagtacttt ttattccatt acaggtaatc tgtcaacaaa ttatggaact gtaatttatc
     3241 aaggcttgac tttaactcag tgtcttaaaa tggaatcgag taccaatatc agctttacaa
     3301 ctacttcgga agctacatta acgctggtat tcaatcctga ttataccggc accattaaaa
     3361 ttgatgggga aatatataat ccttcgttat ctggcggtat agttaccatc acaacacttc
     3421 ctgccggaac tcatactatt ttaaaaggaa gcggatcaag ctatttgttc tacataagta
     3481 cagtttataa tccaatatct attgttgaaa agattcatga ccaaaaactt tcggtagagc
     3541 tttatcccaa tccggtttca actcgattga atatcaactc aaataacaaa gtgacgaaag
     3601 tagaaattta caacgtgtca ggattattag taaaaagctt aaatatgcca ggttctcaaa
     3661 tcgatttgtc aaacctgaag agcggaatat atattgtaaa aatatttacg gaaaataacc
     3721 taataaagaa aacgataata aagaattaaa atggttgaaa atttttatgg aaattaatct
     3781 aaatttgaga aaaagaggaa gttttacaaa accggtaatc aaatttacga agcttaacga
     3841 ttcctctgaa aattttgaat ggtgtctcga cattattcat ttaaataaag aaattcctta
     3901 aaattgacta tcctcgccgc aagcagcgag gtattacgcc aattttatgg cttcgccacc
     3961 ggaatttcta aaaaaacaaa aacaaaagct accctgacgc aagcatcggg gaattcttac
     4021 gattaaaata agtgaaaatt ttttatataa caaactctaa agatcttact ttatcagata
     4081 tatgattttt ttattattct ttgaactaaa ttcgaggaag gtagaaaata cctattttta
     4141 aaaagcctta gaaataaaat aattaatttt atttttatta acaatattta tatccttata
     4201 ttcccgtaat acagagaata tttatacttt tgtgcacgta cacaatcata ctataaaaaa
     4261 tcagtctaaa gcttaatgaa aaatagcttt taaaaaattg agcctgaaaa tcaaagaaat
     4321 ttaaataaat atttttaatg aaaaactatg tacgaattat ttaacattca aaacaaagta
     4381 atcgtgatta ctggaggcac aggcgtgctg gggtcatcta tggttgaata tcttgcagct
     4441 catggtgcaa aaatggccgt tcttgcccgt aataaacaaa aaggcgacga attgatcaat
     4501 cgtgtaaaat caaatggagg agaagccatt ttccttcaaa cagatgtcac caacgaggct
     4561 atacttactc aaaatgcaga agatattgtg gcaaaatacg gtcggattga tgtgttaata
     4621 aatgcagcag gcggaaacat gcccggtgcg atcatcagcc ccgatggtac tatttttgac
     4681 ctgaaaatgg acgattttcg caaagttgtg gatttaaatc tgatgggaac agtaataccc
     4741 accatcattt ttgccaaaat tatggttgcc aataagcaag gaaatatcat caatatttca
     4801 tcggaagctg ctctaaggcc tcttacccgg gtagttggat atgcggcagc aaaagccgcg
     4861 gtgaccaatt tcaccaaata catggcaggt gaattggcaa taaaattcgg agaaaatttc
     4921 agggtaaatg caatcgctcc agggttcttt attacagagc aaaaccgaac gcttttgacc
     4981 aatccagatg gttcatattc agacagagga aaagctatta ttcagcatac gccatttagg
     5041 cgattcgggg ttcccgaaga tttacacgga acattacact atttggtaag cgatgcttct
     5101 aaatttgtaa ccggtacagt tgccgtagtt gacggcggtt ttgatgcatt ttgtatctaa
     5161 aaaaataaag ttatgtcatt attattaaaa aaagattgta aatattcttt acttgttccg
     5221 acaagtatgg gcgtacggat tacacccgtg aatgcccaac ccgttcattg cagtacactc
     5281 ttcgagatgc aggctaccag cgcagaaaca aatgttgcga gcatttcgtc atacctggga
     5341 ctcccggtaa aagtattgac cacattcgta aaagacagtc ccattgccaa gtttattaaa
     5401 gccgaccttc gtcggagaaa tatggatttc gaaggtcccg aagttcctca gggaggccca
     5461 tggggctatc gccatcaatt taatattgcc gacagcggat tcggattaag agggccgcgg
     5521 gtacataatg atcgcgcagg agaagtagga agaacactac atgtgaaaga tttcgatctg
     5581 gaacgaattt tcgctcagga aggtgttcaa attgttcatc tctcgggatt aattgctgcg
     5641 ctttcacccg acacaggcaa tttttgtctt gagatcgctc gttttgcaaa aaaacacggc
     5701 acgctgattt cttttgatct caattaccgg gcttcattct ggaaaggacg cgaaaccgaa
     5761 ctttcagcta tattcaaaga aattgcttcg ctgtcggata ttctcattgg aaacgaagaa
     5821 gattttcaac tttgtcttga tattaaagga cctcaaccag gaggcgagga tattgatgct
     5881 caattagatg catttaaaga aatgatcaaa aatgcccata aaacctaccc caacgcttct
     5941 gtttttgcca atacattgcg agaagtagtc agtgcaaatg aacatctctg gggcgccatt
     6001 gtttatgaaa atgacaagtg gcacattatt agaccaagaa aaatcaatgt gttggatcgt
     6061 ataggcggag gtgacggctt tgtaggtggc ttgctctata gtatcctaaa agaatggcca
     6121 gcagaacaat ggattcagtt tgcatgggca tgcggcgcat tagcaacaac attccttact
     6181 gattatgcac aacctgctga cgaggaaatg atttggaaca tatgggaagg aaatgcacga
     6241 gtaaaacgat aaacatataa aaatttagaa aaatatgaaa aaattagcag atattggact
     6301 gataggtctt gccgtcatgg gtgagaattt ggtgctgaac atggaaagta aaggctttac
     6361 ggtagctgtt tttaaccgaa cagtttcgaa agtcgacgaa tttatcaatg gtagagccaa
     6421 gggtaaaaac attatcggga cacattcact cgaagaactg gtggcttcgt tgagccggcc
     6481 acgcaaagta atgttaatgg taaaagcagg acagccggta gatgatttta tagaaaaact
     6541 aattcccctg ctcgaacccg gtgatattat catcgatggt ggaaacagtt actttatgga
     6601 cactcgtcgc cgtaccaact atgttgaaag taaaggatta ttatatatcg gtaccggtgt
     6661 ctcgggaggc gaagaaggcg cattgcgcgg tccttcaatg atgcccggag gttcttctgc
     6721 cgcatggcct cacgtaaaag atattttcca ggccgttgca gccaaggtcg atgatggcac
     6781 accatgctgc gactgggttg gtgaagatgg ggcaggacat ttcgtgaaaa tggtacataa
     6841 tggcattgaa tatggtgaca tgcaaattat taacgaggca taccatctga tgaaagagtt
     6901 actcggtttg acgactgatg aacaacacga agtgtttaaa aaatggaata ccggaaaact
     6961 caattcctac ctgattgaaa ttacagcaga tattttggct tttaaagatt cggatggcac
     7021 accactggta gaaaaaattt tggatactgc aggtcagaaa ggtacaggaa aatggacagt
     7081 aaacactgct cttgacctgg gaattccatt gacattgatc ggcgaatcag tattcgcccg
     7141 ctgtctatca gcccaaaaag atttgcgtgt gaaggcatca aaaacaattt ccggcacaaa
     7201 acctcatttc aaaggtgaca aacaacgatt tattaatgat ctggaagaag cattgtatgc
     7261 cgcaaaaatt atttcctacg cacaaggata tgatttgatg agagaggcag ccaaagaata
     7321 caactggaat ttaaattatg gtggtatagc cttgatgtgg cgtggcggat gcatcattcg
     7381 ctcagcattt ctgggagata tcaaaaaagc tttcgatcgg aatccttctc ttgagaattt
     7441 gttacttgat ccttttttca aagatgccgt agaatcagca caggaaagct ggcgcagtgt
     7501 atgtacagca gcacttgaaa acggtattcc gattcctgca cttgcttcag cattatgcta
     7561 cttcgacggt ttccgctgtg aacgattacc ggctaatttg ctgcaagctc aacgcgatta
     7621 ttttggtgca catcaatatg aaagaataga cagacctcgt ggcgaatttt tccatacaaa
     7681 ttggacagga cacggagggg atactgcttc ttccacctat caggtataat ttttttaaga
     7741 tactcacagc ataatgtaag gttatgctgt gagtttgtat tttcgaggaa caatgattta
     7801 ctaccaaaac ggctcttctc aacacaatct ctcacacgag gacttgaaaa aaagcctcat
     7861 tgcagcatta gacaaattag gcagaaagca taaaattctt gccattcctc ccgattatac
     7921 acgtttgcca agtcgggcgg gagaactcac cgaaatggtt tgggaatatt acggcaacac
     7981 acttaccgat atactcccgg cattaggcac acacactcct atgacagatg aacaaatttc
     8041 gcacatgttt ggaaaaacac cacgaaattt gtttcgtgtt cacgattggc gtcacgatgt
     8101 aatcacattg ggagaggtcc cagcagaata tgtaaaagaa gtttccgaag ggaaggtaga
     8161 tttttcttgg ccggcacagg taaataaatt gctggtggaa ggtaattttg acctgattct
     8221 ttccataggt caggtggttc cacacgaagt ggtcggtatg gccaattaca acaagaacat
     8281 ttttgtaggc acaggtggta tcgaaggaat taacaaaagc cattttgtcg gtgccgttta
     8341 tgggatggaa cgcatgatgg ggcgcgcaga taccccggta aggaaactgt ttaactatgc
     8401 ctcaaaaaat tttgccaatc agctgcctat tgtttatata ctgacagttg ttggcataaa
     8461 caaggaaggc aagcagcaga cttacggact ttttgtaggt gacgatgtgg atgtattcga
     8521 tgaagcagca aagttgtctc ttgaagtgaa tttcgaaatg gtcgaagaac ccctgaaaaa
     8581 aatccttgtt tggctcgacc ctaccgaatt caaaagcacg tggctgggga ataaatctat
     8641 ctatcgcacc cgcatggcta ttgctgatgg tggggaactt attgttttgg cacccgcctt
     8701 aaaagaattt ggagaggaca aagagattga ccgccttatc agaaaatacg gctatcatgg
     8761 aacttccgct accttgaaag ccatagctga aaacgaagaa ttacggaata atttaagtgc
     8821 tgcagctcat ttgattcatg gctcgtcaga agggcggttt actatcactt actgccccgg
     8881 caaaaatccc gaaaacctga caaaagaaga aatcgaaagc gtgggtttcc aatgggcaga
     8941 tatcgatgag gtgatgaacc gttatcactt tcgtgaactc aaagagggat acaacatctt
     9001 gcccgatgga gaagaaattt tctttatctc caacccggca ttgggactat gggcacataa
     9061 agatcgattt gaattttgaa tttaaactct caagttttgg aataattaat tctgacattt
     9121 attacttgaa agtacttaat aaatccaaat ttgtcattag gtaaaattta cactaacgat
     9181 tattagaaaa taggtatacc aacttacaac cagaatcaaa attaaataaa gaaaacaacg
     9241 acctttgaca tcaaataata tacgattgta ttttgtttta atagtgagtt tgcgctgatt
     9301 cttcacttgc taagttttac gtagatttgt aatctgcaga tacggaaggt aaaaaataat
     9361 ggcacaaatt gcaaatttat gtcagaaaaa atttttaatg ttaatcaaca aaggtattaa
     9421 gtatccgcct ccataaaaaa ttttcctttt ttcgcactac ataccttgaa cttcctccgt
     9481 ttttcagctt agtagacaag attaattact tttcttttac cattttcagc aaaatcattt
     9541 ttctttttga aaacaagttg gtgtaattga ctatcaaagc aaaatacaaa ataaagaaag
     9601 gttttcgtta atttgccagt gaatatgtat atttgtatcg aaaattaatg aataaaatga
     9661 gttatctcca aaaatatagt ttacgtattt gctggatgtt aattttatgg agcatcggaa
     9721 attcttgttc caccactgat gaattttgcc gggaagaaaa gaatgttaaa ttaaaaatga
     9781 gtttttatac tcgtaatttt aatagcaata cacaaaaata tgtcaattct tccttatctg
     9841 tggatagctt gacaattgtt ggcatcgatt ctttaggaca attaattgac tcagttttat
     9901 ataacaattc aaaaaatatt tcatcagtta aattgccctt aaataaattt tcaaaggaat
     9961 caaagtttct attaattttg aatgatgagt tagataccat aacaatacat tataccaatc
    10021 atttacagta cctttcattt gaatgtggaa gtattatcac tcacactatt gacactgttt
    10081 atgctacttc acactttatt gattccctga caattgttga accagacata acaactcttg
    10141 atgttgaaaa tttgcaaata tttcattatt agttttttga ttttcttttt tagtcacata
    10201 aacgtttttt ctcaaaataa aaaagaaaat ttacctaaaa ttaagtggtg gaatggaatt
    10261 atgttgaatt ttgatgtcgt ttctcctatt caatcctctt tatctagttc acaaaatttc
    10321 tcagcggtag gaaatgtaca aataaattta aaggatattt ttttacccgt ttatgaaata
    10381 ggcattgatg gaattaataa aacttcttca caagagattt attacaaagc taatggaatt
    10441 tttcaacgtg caggcataga ttttaattta ttaagacaaa aaaaagaagc tgaaccaagt
    10501 aataacaaat tgttaggtgg aattcgagtt ggatggtcaa attttaagta tgatgtgacc
    10561 aatattacta ttacagacga ttattggggt ggagaaagta tattaaatta tgagcgtcaa
    10621 tcagcttcta aagtctggta tgaaattgtt tttggaattc aagtggaagt atttaaaaac
    10681 gtatatttag ggtggaacat tcgaagcaaa aacttgttca aacaaaatac ttcaacagaa
    10741 gtttctccat ggtatattcc aggttttggt aaaaacaata cttctaatgt tggatttaac
    10801 tatactgttg ggtatcattt ctaataactt aaaaatgaat atattgaata aaaacaacaa
    10861 aactatattt ttatgaattc aattgaaact ttaaatcggg cggctgacaa cattcgtatt
    10921 ttggcagcag caatggtaga aaaagcaaaa tcaggacatc ctggaggcgc catgggaggt
    10981 gccgatttta tgaatgtgct ttattctgaa tttcttgaat atgatccaga aaatcctttt
    11041 tgggaaggac gcgatcgttt ttttcttgat ccagggcaca tgtcaacgat gttatatgca
    11101 gtacttgctt tttctggaaa atttacatta gacgaattaa aacaattcag acaatgggga
    11161 agtcctaccc cgggacaccc tgaactggat ataaaaagag gaattgaaaa tacttctggt
    11221 ccgcttgggc aaggacatac atatgctgta ggagcagcca tagcagcaaa atttttaaaa
    11281 gctcgttttg gtgaagtaat ggatcatacc atttatacgt ttatatctga tggaggcatt
    11341 gaggaagaaa tttcacaggg tgctggacgt attgctggtc atttaggatt ggataatttg
    11401 attatgttct atgattcaaa taatattcaa ctttctaccg aaaccaaagt ggttacgagt
    11461 gaaaatactg ctcaaaaata tgaagcttgg ggctggaagg tgatcactat cgatggcaat
    11521 gatgcagcac aaattcgaaa agctttgaca gaagccaaag ccgagaagga acgacctacg
    11581 ttaattatcg gaaaaactat tatgggaaaa ggaataaaaa aggcagatgg ttcaagtttt
    11641 gaaaatcaaa tatctactca cggtatgcct atcagtgaag cagggggttc tttcgaagaa
    11701 acaataaaac atctcggagg agatcctgct aatccttttg ttatttttga agaaacaaaa
    11761 actttatatg ctaaacgtgc agaagaatta aaagaaattg tttcaaaaaa acaagccatt
    11821 aaaaattctt gggcaaaaga aaatcctcaa ttggctgaaa agttaaaatt tttcttctcc
    11881 agtgaaattc cacctgtaaa gtgggatgct attgttcaaa cccctaacaa atctactcgt
    11941 gctgcttctt cagtggtatt agctgaattg gcaaaacagg ttgaaaatat gattgtagca
    12001 agtgctgatt tgtccaattc tgataaaact gatggattct tgaagcaaac caaagctttt
    12061 gcaaaaaatg atttttcagg tgcgttttta caggtaggtg tttctgaact caccatggct
    12121 gctctttgtg ttggaatgag tttacatgga ggcgtcattc cagcttgtgg aactttcttt
    12181 gtcttttccg attatatgaa accagtagta cgtatggctg ctttgatgga acaacctgtt
    12241 aaatttatct ggacacacga tgcatttcgt gttggagaag atggtcctac gcatcaacct
    12301 gtggaacagg aagcacaaat tcgcttgatg gaaaaattga aaaatcacag tggaaataat
    12361 tcgatgttgg tgcttcgccc tgccgatgca gaggaaacga cagtagtatg gaaattggct
    12421 ttggaaaata ccaaatgtcc aactgcttta atcttttcac gccaagatat tgtgagcctt
    12481 ccagctattg aaaatcgcag aaaagaagct gaacaagcaa gcaggggagc ttatgtagtt
    12541 caatgtgatg ggaatccaga gattattctt gtagcttcag gttctgaagt ttctacctta
    12601 gctgaagcag cagtattact tcggaaggat ggtattaaag tacgtttagt gtccgtacct
    12661 tctgaaggtt tgttccgctt acaaccaatt acctatcagg aaagagtttt accacccaat
    12721 agtaaaattt ttggtcttac agcaggactc ccagtaacac ttcaaggttt ggtaggaaat
    12781 aatggaaaaa tttttggaat ggaaagtttt ggtttttcag ctccatataa agtattggac
    12841 gaaaaacttg gttttacccc tcaaaacatt tacgaacaaa tcaaaaaaat gctataatta
    12901 attttttcaa taaaaggtaa gaagaaaaga atactcactt cttacctttt ttaatttaga
    12961 ttatggatat tgtttctatt attctaattg cttttgggtt agcaatggat tgttttgcag
    13021 tttctatcag taaaggaatt gcagtaaaaa aattttattt ttcatccact ttattaatgg
    13081 ctttcttgtt tggttttttt caagcagcga tgcctttgat tggttatttt gctggattga
    13141 agtttattac agtcattaaa gatagtgatc attggttagc ttttgtgttg ctggcattaa
    13201 taggaggaaa aatgttgttg gaaggaataa aaaagcactc ttcagaaaaa gatgatggag
    13261 aagctcatcc tttttactgg atgaaacttt taacattatc attggctacc agcattgatg
    13321 cattggctac tggaattatt tttgtcccct atccattgat ggtttggaaa gcaattacaa
    13381 ttattggaac tattagtttc ttaatgagta tattaggtat gtatattgga gtacgttttg
    13441 ggaaacgttt tcatgtaaat gtagaaattc ttggtggaat tattttgatt ggaatcggtt
    13501 tcaaaatact tttacaacat actgttttgg tttagctgat aggagaaagg aaagcatctt
    13561 caatatgctc aatacgaaat ccattttttt gtggaattac agaaatgaca tcaaagcgag
    13621 tttcgccttt ccaatcgaat tttaaaatat attcttgagt ggcatttatt atatttcgta
    13681 tttttttctt tgtaatggct tcttgaggat gttcgaaatt ttcagttgaa cgagttttta
    13741 cttctactat taccaataaa ccatcttttt ctgccacaat atccaattca tatttttgaa
    13801 cgtgccaatt cgtatgtcga attttatatc cttttgaaat taaatattcc tgagcacgtt
    13861 tttcaccagt tgctccaact tcattatgaa atgacataaa aactgtaaaa tttattgaaa
    13921 gtatttactc tttgaagaac cttattgact tttcaaaagt aaaaaaactt a
//
LOCUS       DAOSAS010000030        13866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_2985_length_13866_cov_12.570415, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000030 DAOSAS010000000
VERSION     DAOSAS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13866
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2985_length_13866_cov_12.570415"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2050)
                     /gene="gcvP"
                     /locus_tag="PLR72_02425"
     CDS             complement(<1..>2050)
                     /gene="gcvP"
                     /locus_tag="PLR72_02425"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOK36100.1"
                     /translation="VQGIELEIGDYSTVQFSDRHFGAIVQYPNSNGSIEDYSDFVQKA
                     HAADCKVAVAADLLSLALLTPPGEWDADIAFGSSQRWGIPMGFGGPSAAYFATKNEFK
                     REMPGRIIGISKDAYGKRALRMALQTREQHIKREKATSNICTSQALMASMAGFYAVYH
                     GTEGIKTIAQRIHSITTFLNKILPKYGYQQLNKNFFDTLKIVLPKNVSVEKFRKIAEE
                     HEVNFCYYPSGEIGLSINETTNLDDLKILIKIFASAANQVPINIVQNDIMNQTSIDKK
                     FERASAYLTQQVFRLYHTETEMMRYIKKLERKDLSLTDSMIPLGSCTMKLTAASEMLP
                     LSLPYFTNIHPLAPADQTEGYTELINELEKFLCIITGFSACTLQPNSGAAGEFTGLII
                     LRSYFASRGENQRNKVLIPASAHGTNPASAHQAGLEIVIVNCDESGNVDLNDWREKAE
                     MNKGTLFGCMITYPSTHGIFEADIKEMCDIIHQNGGQVYMDGANLNAQVGLTNPAKIG
                     ADACHLNLHKTFAMPHGGGGPGVGPVCVAEHLISFLPDKKFGKNTVSAAPYGNASLLP
                     ITYGYIRMMGAEGLTKASKIAILNANYLAEQLKDTYGIVYRGKNGRIGHELILECRNF
                     KANAEITENDIAKRLMDYGFHAPTQSFPIHGTLMVEPTESESKKELDRFVQAMLQIYN
                     "
     assembly_gap    2051..2150
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2151..2458)
                     /locus_tag="PLR72_02430"
     CDS             complement(<2151..2458)
                     /locus_tag="PLR72_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HOK36101.1"
                     /translation="MKDMLAARHIGISENEEEKMLETLGLKSLDELIQQTIPNSILLK
                     ETLDLPEPLTEHQYSKHILQLGLKNKLFTSYIGQGWYDTCTPAVIFRNVFENPVWYTS
                     "
     gene            complement(2550..>3341)
                     /locus_tag="PLR72_02435"
     CDS             complement(2550..>3341)
                     /locus_tag="PLR72_02435"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOK36102.1"
                     /translation="YKLSAQDIKNFMRDQYEGTELDITKGLGAGPFNSKLRCSPLTFM
                     VDSVEYVHERPVATQQTAFSFVAQMRNWLPNYVGGILWFGVDDAASSLYVPMYCGIHQ
                     IPDCYDIHNGSLLKYSPTSAFWIYNQVANFAYSKYSYMMKDIKKVQSTWESKFNELVP
                     NVDKIAANLPETTARDFLTDFSCLQATSSTQAWKKLGEYLLVKYMDGNVKMEKDGKFL
                     QNEKGIPPDIIRPGYPEDFLRQMVKENPQLRVKTIKELKENNIIP"
     assembly_gap    3342..3441
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3442..4013)
                     /locus_tag="PLR72_02440"
     CDS             complement(<3442..4013)
                     /locus_tag="PLR72_02440"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOK36103.1"
                     /translation="MNKKLASTLLLSITYINIFACTNYLIGKSATVDGSTIITYAADS
                     YFSYGYLYYSPAATYSPNTLRDIYEWDSGKYLGKIKQAEKTYAVVGNMNEYQVSIGET
                     TFGGRLELMDTTATLDYGSLIYLALQRSKTAREAIQVMTELVTEYGYRSEGESFSIGD
                     PNEIWILEMIGKGPKNKGAVWVARRIPDDCV"
     gene            complement(4075..5508)
                     /gene="cls"
                     /locus_tag="PLR72_02445"
     CDS             complement(4075..5508)
                     /gene="cls"
                     /locus_tag="PLR72_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOK36104.1"
                     /translation="MSKFITIFFIALYVYTVLSTISVLLLENRNPAKSFSWMLIILFL
                     PVIGLLLYITFGQDLRKQKIISRKSLHNLPERVIAPFDITEVEQLLNNLEKCQKNLIK
                     LLYKNSEAIAYPANKIQVYPDGESAFDAIFDAIRAAKNHIHIEFYILSNDKVAQRLKE
                     LLIQKSHEGVRVRVIYDYIGSLNLSRKYIKSLTKAGVYVRPFLPLRLRIFRSRINYRD
                     HRKIMIIDGKIGFTGGMNIADRYIKGNRLGKWRDNFVRFEGAVVHGLQRLFLADWYFV
                     QGKLITDTKYYPQPENFNTNNLIQIVSSGPDTDWESIMQGIASAIMTASKYVYIQSPY
                     YMPPELIECCIQIASLSGVDVRLMIPTRSDSIIAIKSTSSYLGKILEAGVKVYYYKTG
                     FLHSKMLVIDDMISIVGSCNIDYRSFTQNFEANAFIYEKETAIQLKELFIKDIQQCEE
                     MTLEKWANRSKFQKMEESFSRLFSPIL"
     gene            complement(5516..6559)
                     /gene="ribD"
                     /locus_tag="PLR72_02450"
     CDS             complement(5516..6559)
                     /gene="ribD"
                     /locus_tag="PLR72_02450"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOK36105.1"
                     /translation="MTEEEKYIARCLQIASYGDGFVAPNPLVGAVLVNEEKIIGEGFH
                     QYFGQAHAERNAILSVKDPEQLKNATLYVNLEPCSHYGHTPPCANFIVDCKIPKVVIG
                     TYDPNPDIYGQGVEILQKAGIELKIGVLEKECKELNKRFFTFHEKKRPFVTLKWAQTI
                     DGFMDKNRTNVQQALLLISNNFTRVLAHKMRSENQAILVGTNTVLLDNPSLTVRYWSG
                     KTPVRIVLDRLGKIPENYHLLNNSTPTIIFTEKPYQEKLNVKYIPIDFDKNILSTVLQ
                     KIYELNIHSILVEGGAQLLTNFIISNLWDEAYVEVAPFEISEGVTAPIIPLMPFARKN
                     FYGHEWYHYKNKK"
     gene            6646..7476
                     /gene="prmC"
                     /locus_tag="PLR72_02455"
     CDS             6646..7476
                     /gene="prmC"
                     /locus_tag="PLR72_02455"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOK36106.1"
                     /translation="MKYIQTELKGYYPDNEIQSFGYLLLEKIMGLSRSKILINKNTKF
                     SSEQWEKLNSFLQQLKEYVPIQYILGETEFYGLKFLVNSSVLIPRPETEELVEWITKE
                     NDKNSALQILDIGTGSGCLAISLKHEFPFASVDAFDVSHEAIETARLNAEINHTEIHF
                     FEADILKNPLFDKKWDIIVSNPPYVPENEKEILLPNVLHYEPSMALFVPKNDPLLFYR
                     NVAEFAQIHLNQGGKLYFEIHYKAGENMVTLLEQESFQQIELKKDLSGNDRMIRAIYH
                     "
     gene            complement(7551..8183)
                     /locus_tag="PLR72_02460"
     CDS             complement(7551..8183)
                     /locus_tag="PLR72_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOK36107.1"
                     /translation="MNENFVITIGRQIGSGGHQIGNEIAKYFNIDFYDKELIQAVSKE
                     TGLAKEIFDEMDEKKTHSIFSNFLGLQNSVMMDQFYLCDNYLSNETLFKIQSEVIRKL
                     ADQKSCVFVGRCADYVLKDYPRIVTIFITADFEDRIKRIMERENLSTEKAKEYLKKME
                     KKRADYYNYFSNKQWGAAASYQLCINSSALGVEKTVDYLIEFIKQKLQLE"
     gene            complement(8176..9561)
                     /locus_tag="PLR72_02465"
     CDS             complement(8176..9561)
                     /locus_tag="PLR72_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOK36108.1"
                     /translation="MELKGLPLTLGTEKIRNLLIQYSLPAIIAMTASSLYNITDSIFI
                     GHGVGALAISGLAITFPFMNLSAAFGSLVGVGAATLMSVRLGQKDYSTANQILGNVFV
                     LNIIFGLAFAITSLIFLDPILYFFGASPATLPYAHDFMVIILIGNVITHIYLGLNGLL
                     RSIGKPEKAMYATLTTVGLNLILNPLFIFGFKWGIRGSALATVLSQTILLIWQIKLFS
                     DKENFIHFQKGIFKLNKRIVKESLAIGMSPFLMNAVGSIIVIVLNQSLIRHGGDLAVG
                     AYGIINRVTFLFAMVVVGLNQGMQPIAGYNFGAQKFDRVNEVLKLTILFATAIMTVGF
                     LIGELFPRGVASIFTKDKELIRLASEGLRITFIFFPIVGFQMVTTNFFQSIGMAGKAV
                     FLSLTRQLIYLLPCLLIFPSIYGLKGVWYSMPVADLLATLTAAYMLFVNYRKNISKSK
                     LISQTKSYQNE"
     gene            9738..10220
                     /locus_tag="PLR72_02470"
     CDS             9738..10220
                     /locus_tag="PLR72_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOK36109.1"
                     /translation="MKKEFSVDELFQRAASYCSLSEHCVSEIDQKLTSWKVEKSLKKK
                     LIDKLLANDFINEKRYAKAFVNDKFRFNKWGKIKIALALSSKGLNKELIDEALSSVDE
                     EEYLSILENLLKSKLATFKDLTDYEKKGKLFRFAQGKGFEPTAIEKVLQRLEENGAFR
                     "
     gene            10353..10583
                     /locus_tag="PLR72_02475"
     CDS             10353..10583
                     /locus_tag="PLR72_02475"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOK36110.1"
                     /translation="MKTNVGGNDKILRLGVAIVLIILFYKSVLKGTLGLIGLIIAFLL
                     TLSSLIGFSFLYSIFKIDTSKKKEKEKRKPEK"
     gene            10793..11794
                     /gene="gap"
                     /locus_tag="PLR72_02480"
     CDS             10793..11794
                     /gene="gap"
                     /locus_tag="PLR72_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOK36111.1"
                     /translation="MIKVGINGFGRIGRLVFRAAQERNDIQVVAVNDPFLDMDYLIYM
                     LKFDSVHGKFEGTAEKKDGKLFVNGKEVVFFAEKDPVNIGWAATGAEYIVESTGVFTT
                     MEKASAHLKAGAKKVVITAPSNDAPMFVCGVNLDKYTPDMNIVSNASCTTNCLAPLAK
                     VIHENFGIVEGLMTTIHAATNTQKTVDAPSNKDWRGGRSILNNIIPSSTGAAKAVGKV
                     IPSLNGKLTGMSMRVPVSDVSVVDLTCRLEKAATYEEIKAAVKAASENELKGILGYTE
                     DAVVSTDFIHDPRTSIFDANAGIALNSNFVKLISWYDNEWGYSNKVLDLIQHMASVK"
     gene            complement(12045..12920)
                     /locus_tag="PLR72_02485"
     CDS             complement(12045..12920)
                     /locus_tag="PLR72_02485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOK36112.1"
                     /translation="MRQLKGIGKNFGILSQLLLLFGIACVFFYLAVAILMLVGKTNAS
                     DINSIRWLQLIQSVGIFIIPPFIFSYFCSEQPLDYLSLNKTTNSLAVRYVVLIMIVCI
                     PFIDLLTQLNQQMQLPEAFSSIENWMKSTEEEAARVTEQLLDVKGIDGLLLNLLLIAL
                     IPAFGEELFFRGTLQKIFNNWKKPQLAIWITAILFSAFHLQFYGFLPRLLLGAFFGYL
                     LWWSGSLWLPVIAHFANNALAIIFSYINQREIQILDPNAVGQHGNIWLGIASAVLSGY
                     LIFRLQKNILSHTKS"
     gene            complement(13004..13249)
                     /locus_tag="PLR72_02490"
     CDS             complement(13004..13249)
                     /locus_tag="PLR72_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOK36113.1"
                     /translation="MNIYVGNLSYKVRENDLQGVMEEYGQVTSCKIVKDRDTGRSKGF
                     GFVEMSDDAAATKAIEELNGAEFDGRTMVVKEARPKN"
BASE COUNT         4574 a         2275 c         2265 g         4552 t
ORIGIN      
        1 tcattgtaaa tctgcagcat agcttgaaca aaacggtcta attctttttt tgattcgctt
       61 tctgtgggtt ctaccattaa agttccatga ataggaaatg attgagtagg agcgtgaaaa
      121 ccataatcca ttaatcgttt ggctatgtcg ttttctgtaa tttcagcatt tgctttgaaa
      181 tttcgacatt ccagaataag ttcatgacca attctgccat tttttcctcg ataaacaatt
      241 ccataagtat ctttcaattg ttctgccaga taatttgcat ttaaaatggc aatttttgaa
      301 gcttttgtca gaccttcagc tcccatcatg cgaatgtatc cataagtaat gggcaaaaga
      361 cttgcattcc cataaggagc agccgaaacc gtatttttcc caaatttttt atcgggtaag
      421 aaagaaatta aatgttcagc tacacaaaca ggtccaactc caggtccgcc gccaccatgt
      481 ggcatagcaa aagtcttatg caaattcaaa tggcaagcat cagcaccaat ttttgcagga
      541 ttagttaatc caacttgtgc atttaggttg gcaccgtcca tatacacctg accgccattt
      601 tgatggataa tgtcgcacat ttcttttatg tcggcttcaa aaattccgtg tgtggaagga
      661 taggtaatca tgcaaccaaa caaagttcct ttgttcattt cagctttttc tctccaatcg
      721 ttcaaatcga cgttgccact ctcatcgcaa ttaacgataa caatttccag gcctgcttga
      781 tgtgccgaag caggattggt tccatgagcc gaagcaggaa ttagcacttt gtttcgctga
      841 ttttcgccac gtgaagcaaa ataagaacgt aaaatgatta aaccagtaaa ttctccggct
      901 gctccagaat ttggttgtaa ggtacaagcc gaaaatccag tgataatgca taaaaacttt
      961 tcaagttcgt ttatcagttc cgtgtaacct tctgtttggt cagcaggtgc gagtggatga
     1021 atgtttgtaa aataaggcaa actgagcggt aacatttcag aggcggctgt aagtttcatg
     1081 gtgcacgaac caagtggaat catagaatct gtcaaagaaa gatctttacg ttccagcttt
     1141 ttgatataac gcatcatttc tgtttccgta tgataaagtc ggaatacttg ctgtgtaaga
     1201 taagcagaag cacgttcaaa ttttttatcg atagaagttt gattcattat atcgttttgc
     1261 acaatattaa taggaacttg atttgcggct gaagcaaaaa tttttattaa tatttttagg
     1321 tcatctaaat tggtggtttc gttgatactc aatccgattt ctcctgacgg ataataacaa
     1381 aagttcactt cgtgttcttc ggcaattttt ctaaattttt ctactgaaac attctttggc
     1441 aagacaattt tcaaggtatc aaaaaagttt ttatttaatt gctgataacc atattttggc
     1501 aaaattttgt tcaaaaacgt ggttatggaa tggatgcgtt gagctattgt tttgattcct
     1561 tcagttccgt gatagacagc ataaaaacca gccatagaag ccataagagc ttgtgaagtg
     1621 caaatattgg aggtggcttt ttcgcgttta atatgctgtt ctcgtgtttg taaagccata
     1681 cgaagagctc tttttccgta agcatctttg ctaatgccaa taattctgcc cggcatttcc
     1741 cgtttgaatt cattttttgt tgcgaaataa gctgcagaag gtcctccaaa tcccatagga
     1801 atgccccagc gttgtgaact accaaaagca atatctgcat cccattctcc tggaggtgtt
     1861 aaaagagcta aacttagcaa atcggctgct acagctactt tgcagtcggc agcgtgtgct
     1921 ttctgaacaa aatctgaata atcttcaata ctgccatttg aattgggata ttgaacaatt
     1981 gccccaaaat gtctgtcaga gaattgaaca gtggaataat cgcctatttc caattcaatt
     2041 ccttgaactt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taagaagtgt
     2161 accagacagg gttttcaaaa acattgcgaa aaattacggc tggtgtgcag gtgtcgtacc
     2221 atccttgacc aatataggaa gtaaacaatt tatttttcaa gccaagctga agaatatgtt
     2281 ttgaatattg atgttctgtt aaaggttcag gtaaatcgag tgtttcttta agcagaattg
     2341 agtttggaat ggtttgctga attaattcgt ccaaactttt caagccaagc gtttccagca
     2401 ttttttcttc ttcattttct gaaattccga tgtggcgagc agcaagcata tctttcatag
     2461 aatgattcat ttgcaattta taatttattg aaaattagta ttttattatt ttattgagaa
     2521 gctattgcct taaaattttc ctgaaaaaat taaggaataa tattgttttc tttcaattct
     2581 ttgatagttt ttactcgaag ttgtggattt tctttcacca tttgccgtaa aaaatcttct
     2641 ggataaccag gacgaataat atcaggcggg atgccttttt cattctgaag aaattttcca
     2701 tctttttcca tttttacatt gccatccatg tattttacca gcaaatattc tcctaatttt
     2761 ttccaggctt gtgtagaaga agtggcttgc aagcaactga aatccgttaa aaaatcacgt
     2821 gctgttgttt caggtaaatt ggcagcaatt ttatccacat tgggaaccaa ttcattgaat
     2881 ttactttccc acgttgactg gacttttttt atgtctttca tcatataaga atacttgctg
     2941 taagcgaaat tagcgacctg gttgtaaatc cagaaagctg aagtgggcga atatttcagc
     3001 aggcttccgt tatgaatgtc gtagcaatca gggatttgat gaatgccgca atacattgga
     3061 acatacaaag aactggctgc atcgtccacg ccaaaccata aaattccgcc aacgtagtta
     3121 gggagccagt ttcgcatttg tgctacgaat gaaaaagctg tttgttgtgt agctacaggt
     3181 cgttcgtgaa catattccac actatcgacc ataaaagtca gcgggctaca acgcaattta
     3241 ctgttaaaag gtccggcacc caaacctttg gtaatatcca gttctgtccc ttcatattga
     3301 tcacgcatga aattttttat atcttgagca cttaatttgt annnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nacgcaatca tctggaattc gtcgagctac ccagacagca
     3481 cctttatttt tcggaccttt gccaatcatt tccaatatcc agatttcgtt tggatcgcca
     3541 atagaaaatg attctccttc acttcgataa ccatattccg tcaccagttc tgtcattact
     3601 tgaatagctt cacgagcagt tttcgaccgt tgcagtgcaa gataaatcaa gcttccgtaa
     3661 tcaagtgtgg cagttgtgtc cattaattcc aaccgtcctc caaaagtcgt ttcgccaatt
     3721 gacacctgat attcattcat attgccaaca actgcataag ttttttctgc ttgttttatt
     3781 ttccctagat atttgccgga atcccattca taaatgtccc tcaaagtatt gggagaatac
     3841 gttgctgctg gagaataata taaatatccg taagaaaaat aggaatcggc agcataagtt
     3901 attatcgtag aaccatcaac cgttgcagat tttcctatta aataatttgt gcaggcaaaa
     3961 atatttatat aagttattga aagtaaaaga gtggaagcta atttcttatt cataaggaaa
     4021 attattcaaa ggataagtga aaaattgttt taataatgtt ttttaaataa atattcataa
     4081 aattggactg aaaagacgcg aaaatgattc ttccattttc tgaaatttgc ttcggtttgc
     4141 ccatttttcc agtgtcattt cttcacattg ttgaatatct tttataaaaa gttcttttaa
     4201 ttggatagca gtttcttttt cataaataaa agcatttgct tcaaaatttt gtgtaaagct
     4261 tcgataatct atattgcaag atcctacaat tgaaatcata tcgtcaatta ccaacatttt
     4321 gctatgcaga aaaccagttt tataatagta aacttttacg cccgcttcca gaattttgcc
     4381 aagataactg gaagtgcttt taatagcaat aatagaatcg gaacgcgtag gaatcataag
     4441 tcgtacatca actccactta acgaagctat ctggatgcag cattcaatta attctggtgg
     4501 catataataa ggcgattgaa tataaacgta tttactggca gtcattatag ctgaagcgat
     4561 tccttgcatg atagattccc agtcggtatc aggtccactg gaaacaattt gaatcaaatt
     4621 attggtgttg aaattttcag gttgaggata atatttggta tcggtaatta attttccttg
     4681 aacaaaatac caatcagcaa gaaataaacg ttgcaagcca tgtactactg ctccttcaaa
     4741 ccgaacaaaa ttatcgcgcc atttacctag tcgattgcct ttaatgtatc gatcagcaat
     4801 gttcattccg cctgtgaaac caattttccc atcaataatc ataatttttc tgtgatctcg
     4861 gtagttgatt ctgcttctaa aaattcttaa acgtaatggt aaaaaaggac gaacataaac
     4921 tcctgcttta gtcaaggatt ttatatattt tcttgataag ttcaagcttc caatataatc
     4981 ataaattact cggacacgaa caccttcatg agatttttga atcaacaatt cttttaatcg
     5041 ttgagcaact ttatcattac ttaaaatata aaattcaatg tggatatgat ttttagctgc
     5101 acgaatggca tcaaaaatgg cgtcaaaagc actttcgcca tccgggtaaa cttgaatttt
     5161 attagcagga taagcaatgg cttcactgtt tttatataaa agtttgatga gatttttctg
     5221 acatttttca aggttattca ataattgttc tacttctgta atatcaaaag gagcaatgac
     5281 tcgttcaggt aagttatgaa gactttttct ggaaataatt ttttgtttgc gtaaatcttg
     5341 tccaaaagtt atataaagca gcaagccaat tacaggaagg aacaagatga tcagcatcca
     5401 cgaaaatgat tttgctggat ttctattttc gagcaacaat acagaaatag tactgagaac
     5461 agtatagacg taaagagcaa taaagaatat agtaatgaat tttgacattt cttatttatt
     5521 ttttattctt ataatgatac cattcgtgtc cataaaaatt ttttcgtgca aaaggcatta
     5581 gcggtataat aggtgctgta acaccttcac tgatttcaaa aggtgctact tctacataag
     5641 cttcgtccca taagttggaa ataataaaat ttgttaataa ttgagcaccg ccttctacta
     5701 aaatggaatg aatattcagt tcataaatct tttgtaaaac agttgataag atatttttat
     5761 caaaatctat tggaatatat tttacattta atttttcctg ataaggtttt tcagtaaaaa
     5821 ttattgttgg tgtagagtta tttagcaaat gataattttc cggtattttc cccaagcgat
     5881 ccagcactat tctgacaggt gtttttcctg accaatatcg aacggttaag gaaggattat
     5941 ctaaaagaac agtattagta ccaacaagaa ttgcctgatt ttctgaacgc attttatgag
     6001 caagaaccct agtaaaatta ttggaaatca acaaaagagc ttgttgaaca tttgttctgt
     6061 ttttgtccat aaatccatca atagtttgag cccattttag tgtaacaaat ggacgtttct
     6121 tttcatgaaa agtaaaaaaa cgtttgttta attctttaca ttctttttct aaaacgccta
     6181 tttttaattc aatccctgct ttttgaagaa tttctacacc ttgtccataa atatctggat
     6241 taggatcata ggtgccaatc actacttttg gaattttaca atctacaata aaattggcgc
     6301 atggaggtgt gtgtccataa tgagagcaag gttcaaggtt tacatataaa gttgcattct
     6361 ttaattgttc aggatctttt accgaaagaa tggcattccg ttcagcatga gcttgaccaa
     6421 aatattggtg aaaaccttcg ccaataattt tttcttcgtt aaccaaaact gctcctacca
     6481 atggattggg agctacaaag ccatctccat aggaagctat ttgtaaacag cgtgctatat
     6541 atttttcttc ttcagtcatg ttcccagtaa aaacctgatt ttaaaaataa tgttgttatt
     6601 cacttgtttt tacaaattgt ttatttttgc attatggaag aaataatgaa atatattcaa
     6661 actgaattaa aaggttacta tcctgacaat gagattcaaa gttttggtta tttattgtta
     6721 gaaaaaataa tgggactttc acgttctaaa attttaatta acaaaaatac aaaattttct
     6781 tctgaacaat gggaaaaatt aaattcattt cttcaacagc taaaagaata cgttccaatt
     6841 caatatattt taggcgaaac agaattttat ggtttgaaat ttttagtaaa ttcttcagta
     6901 ttgattccac gtccagaaac ggaagaatta gttgaatgga tcacaaaaga aaatgataaa
     6961 aattcagctt tgcaaatttt agatatcggt actggaagtg gttgccttgc tatcagtttg
     7021 aagcatgaat ttccttttgc cagcgttgat gcttttgatg tttcacatga ggcaatcgaa
     7081 acagcccgcc tgaatgctga aatcaatcat acagaaattc attttttcga agcagatatt
     7141 ttaaaaaatc cactttttga taaaaaatgg gacattattg tcagcaatcc accctacgtt
     7201 ccagaaaatg aaaaagaaat tcttttgcca aatgttttgc attatgaacc ttcgatggca
     7261 ttgtttgttc ctaaaaatga tcctttgttg ttttacagga atgttgccga atttgctcaa
     7321 attcatttga atcagggtgg aaaactttat tttgaaattc attacaaagc aggagaaaat
     7381 atggtaacat tgctcgaaca agaaagcttt cagcagattg aactaaaaaa agatttgtca
     7441 ggaaatgata gaatgatcag agctatttat cattaagtga agggaattat ttttatttaa
     7501 atggataaaa ataatttttt gcttttaaaa tcatgctaaa aagctatcaa tcattcgagt
     7561 tgtaattttt gtttgataaa ttcaataaga taatcaaccg ttttttctac accaagagct
     7621 gaagaattga tgcatagttg atacgaagca gcagctcccc attgtttgtt gctaaaataa
     7681 ttatagtagt cggctctttt tttctccatt ttttttagat actctttggc tttttcagtg
     7741 gaaagatttt cccgttccat aattcttttg attcgatctt caaaatcggc agtaataaaa
     7801 atagttacta ttctgggata atcttttaat acataatctg cacatctgcc tacaaataca
     7861 caagattttt gatcggcgag cttacgaatt acttcacttt gaattttaaa taaagtttcg
     7921 ttggagagat aattatcgca taaatagaat tgatccatca tcacagaatt ttgcagtcct
     7981 aaaaagttgc tgaaaatgct atgggttttc ttctcatcca tttcatcaaa aatttctttt
     8041 gctaaaccag tttcctttga aactgcctga atcagctcct tgtcgtaaaa atcgatattg
     8101 aaatattttg ctatttcgtt tccaatttga tggccaccac tgccaatttg acgaccaatg
     8161 gtgatgacaa aattttcatt cattttgata actttttgtt tgtgaaatta attttgactt
     8221 tgaaatattt tttcgataat tgacaaaaag catataagct gctgtaagtg tggcaagtaa
     8281 atcagcaaca ggcatgctgt accaaactcc ttttagtcca taaatagaag gaaaaatgag
     8341 taaacaaggt aataaatata tcaattgtct ggtcaaagat aaaaaaactg ccttccctgc
     8401 cattcctata ctttgaaaaa aattagttgt taccatttga aatccaacaa tgggaaagaa
     8461 aataaaagta attcgtaaac cttcacttgc taatcggatg agttctttat ccttcgtaaa
     8521 aatagacgct acacctctgg ggaacagttc tccgattaaa aaacctactg tcattatagc
     8581 agtagcaaag agaatggtaa gcttaagtac ttcatttact ctgtcgaatt tctgagctcc
     8641 aaaattatat cctgcgatgg gttgcattcc ctgatttaat cctactacca ccatagcaaa
     8701 aagaaaagtt acccgattaa taatcccata cgctccaact gctaaatcgc ctccatgcct
     8761 gatcaacgat tgatttaaaa caataacaat aatgctaccc actgcgttca ttaaaaaagg
     8821 tgacatccca atagctaacg attcttttac gatcctttta tttagtttaa aaataccctt
     8881 ttgaaaatga ataaaatttt ctttatcact aaaaagtttt atttgccaaa taagcaaaat
     8941 ggtttgtgaa agaacggttg ccaaagcact tccacgaatt ccccatttaa aaccaaaaat
     9001 aaaaagagga tttaatatca ggttcaaacc taccgtagtt aaggtggcat acatggcttt
     9061 ttcaggtttt cctatcgaac gcagcaaacc attcaagccc agataaatat gagtaattac
     9121 attgccaatc agaataataa ccataaaatc atgggcataa ggtaaagtgg caggactagc
     9181 accaaaaaaa taaagaatag gatctagaaa aataagagaa gtaatcgcga aagccaaccc
     9241 aaaaattata ttcaatacaa atacatttcc taaaatttga ttagcagtgc tgtagtcctt
     9301 ttgtcccaaa cgaaccgaca tcaaagtggc tgctccaaca cctaccaacg aaccaaaagc
     9361 cgcagaaaga ttcatgaaag ggaaagtaat ggccagaccc gatatagcca atgccccaac
     9421 gccatgaccg ataaaaatgc tgtcagtaat gttataaagc gacgacgccg tcatagcaat
     9481 aatggctggc aatgaatatt gaatcagtaa attacgaatc ttttcagtcc ctaaggtgag
     9541 cggtaatcct tttaattcca tacgcctttt tacttctaaa ttgtggcaaa attacaaaaa
     9601 atgctggttt gttgtaatcg ttttttgcaa tgacttatag aaggtgtttt taaagaataa
     9661 ataatctaat caattgataa ataaaaaact tagaaaaata tttgttattt ttgtttgttt
     9721 tttacaagta atcgaatatg aaaaaagaat tttctgttga tgaattattt caaagagcag
     9781 cttcttattg ttcgctttca gaacactgtg tgtctgaaat agatcagaaa ttgacaagtt
     9841 ggaaggtgga aaaatctttg aagaaaaagc taattgataa attgctggca aatgatttta
     9901 tcaatgaaaa aagatatgct aaagcttttg ttaatgataa attccgattc aacaagtggg
     9961 gcaagataaa aattgcttta gccttgagtt ccaaaggttt gaataaagaa ttgattgatg
    10021 aagctctttc atctgttgac gaagaagaat atttgagcat attggaaaat ctgttaaagt
    10081 caaagttagc aacttttaaa gacttgacgg attatgaaaa aaaaggaaaa ctttttcgtt
    10141 ttgctcaggg aaaaggtttt gaaccaacag cgattgaaaa ggtgcttcag cgtttagaag
    10201 agaatggagc ttttcgttaa acaaaagaat aaattttttg aatctgattc ggtttttatt
    10261 tttcaaagtc atttattatt tggtatgtat ttattgaact tctcttataa aaagtttgtt
    10321 ttaattaaaa ataaatttat aaaaaaatac ccatgaaaac aaatgttggt ggtaatgata
    10381 aaattttgcg attgggcgtg gctatcgtat tgattatact tttttataaa agtgtattaa
    10441 aaggaacgtt aggtctgatt ggcttgataa ttgctttctt attaacactt tccagcttaa
    10501 ttggtttttc ttttctatat tctattttta aaatcgatac ttcaaagaaa aaggaaaaag
    10561 aaaaaagaaa accagaaaaa taaaagtaag aaaaaataaa tattcatttt ttctacaata
    10621 aaaagtgaga gaaaagaata tagtgattaa attttattga aataaattaa aatgtaagat
    10681 ttaattacat aaaaatttct ttaattataa tttgaagtgt tttgagaatc aaacataatt
    10741 ctgtaatttt gcacagattt aaagtaaact aattttaaaa ataagtaata aaatgattaa
    10801 agtaggtatt aatggttttg gccgcattgg acgattggta ttccgtgctg ctcaagaaag
    10861 aaacgacatt caagtcgttg cagtgaatga tccattcttg gatatggatt atctgattta
    10921 tatgttgaaa tttgatagcg tacatggtaa atttgaaggt accgctgaaa aaaaagatgg
    10981 taaacttttc gttaatggta aagaagtagt tttctttgct gaaaaggacc ctgtaaatat
    11041 cggttgggct gctactggtg ctgaatacat tgttgaatct acaggagtat tcaccacaat
    11101 ggaaaaagct tctgctcatt taaaagcagg tgcaaaaaaa gtagtcatta cagctccttc
    11161 caatgatgct ccaatgttcg tttgcggtgt taatctggat aaatacactc ccgacatgaa
    11221 cattgtttca aatgcttctt gtacaactaa ttgccttgct ccattagcaa aagtaattca
    11281 tgaaaatttt ggtatcgtag aaggtttgat gacaactatt catgctgcta ccaatactca
    11341 gaaaacagtt gatgctcctt caaataaaga ttggagaggc ggtcgttcta tcttgaacaa
    11401 tattattcct tcttcaactg gtgcagcaaa agccgtaggt aaagttattc cttccttaaa
    11461 tggcaaactg acagggatgt caatgcgtgt tccagtatct gatgtatcag tggttgattt
    11521 gacttgccgg ttagaaaaag ctgccactta tgaagaaata aaagccgctg taaaagctgc
    11581 ttctgaaaat gaattaaaag gaattttggg ctatacggaa gatgcagttg tatccactga
    11641 tttcattcac gatccgcgta cctctatttt tgatgccaat gctggaattg ccctaaatag
    11701 taatttcgta aaattgatta gttggtacga caatgaatgg ggttattcaa acaaagtgct
    11761 tgatttaatt caacacatgg cttctgtaaa atagtttttt cataaactta aataaaaact
    11821 ccctgaaaac taaatatttt aagggagttt tttatttcat acattatacg agattcgaga
    11881 tgcgggaggc gagttttgag tagaaacgtt tcatgaaacg tctcctgtaa aatagaatga
    11941 ggaaaaaaat ttcagtattt ctctgtgtaa taaaccgttc attttcactg atttccacaa
    12001 gtaataaaat atcggattca tatccaacaa ggagagaatt tctgttaact ttttgtatgt
    12061 gataaaatat tcttctgaag gcgaaaaatt aaataaccac ttaaaaccgc actcgctatt
    12121 cctaaccaga tatttccatg ttgtccgacg gcattaggat ctaaaatttg aatttctcgt
    12181 tgatttatat aggaaaaaat aattgcaagt gcattattgg caaaatgagc aataacaggc
    12241 aaccacagac ttccactcca ccataaaaga tacccgaaaa aagctcctaa caacaatctt
    12301 ggtaaaaatc cataaaattg taaatgaaaa gcactaaata aaatggcagt aatccagatg
    12361 gcaagttgcg gttttttcca gttgttgaaa attttttgca aagttcctct gaaaaatagt
    12421 tcttctccaa aagctggaat caaagcaatg agcaataaat tcaatagtaa accgtcaata
    12481 ccttttacat ccagtaattg ttctgtaact cttgctgcct cttcttctgt agatttcatc
    12541 caattttcta tggaagaaaa agcttcgggc aattgcattt gttgattgag ttgcgtgagt
    12601 aaatctatga aaggaataca gacaatcatt atcagtacta cataacgaac agctaatgaa
    12661 ttggttgttt tattgagtga aagatagtca agaggttgtt cactacagaa atagctgaaa
    12721 ataaaaggag gaatgataaa aattccaaca gattgaatca attgtaacca tcttatggaa
    12781 ttaatatccg aagcattggt tttacctacc agcatcaaaa tagccactgc aagataaaaa
    12841 aatacacaag caataccaaa cagcagtaaa agttgagata atattccaaa attttttcct
    12901 atccccttca gttgacgcat atataattga ttgtcaatca ataaaataaa aaaacttcta
    12961 tcatctttcg acaacagaag tttctttctc tgaaatgaat tctttaattc ttaggtcttg
    13021 cttccttaac caccatggtg cggccatcaa attcagctcc gttcagctct tcgatagctt
    13081 ttgttgctgc agcatcatca ctcatttcaa caaatccaaa tcccttagat cttcctgtgt
    13141 cacgatcttt aacaatctta caagatgtta cttgaccgta ctcttccata accccttgaa
    13201 ggtcattttc ccgaacttta tagctcaggt ttccaacgta aatgttcata aaaaataaaa
    13261 ataaaaaatt aaataaatgt ttgtgattta agaaattgta taagcaaaca aacgaggtta
    13321 aaacattcta cccaagaaat ttggaaagga aaaaataacc atttgagaga aagatacact
    13381 tcttataatc gatgtgcaaa gtaaatattt ttttttattt cttgcaagaa tccatcaaaa
    13441 atttaacaaa agggaataaa atgagtcatc aaattttttt tagttacatt aaaaaaataa
    13501 gaaaaattta tacacttttt gatttttatt attacatttg tttcgtacaa ataagggttt
    13561 aaaaaattta tttataagga gtaagagagc ttttcaatgc gaataatcaa atcttaaaaa
    13621 aagtttattt ttcaaatcac ttaaatacat ttgttataaa catttttctt atttttctat
    13681 atattttttt gattcagaca tttttttgaa attatttaaa aaattgtaat ccaataaatt
    13741 gcttttattt tttttatttt ttgaaggtag agatattttt taaccaaaaa tttattttga
    13801 cattaaaggt ttatcaaaac aaagtaataa ttatctctct aattgcttaa ttattcaaat
    13861 ttttct
//
LOCUS       DAOSAS010000031        13671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3039_length_13671_cov_13.105538, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000031 DAOSAS010000000
VERSION     DAOSAS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13671
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3039_length_13671_cov_13.105538"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..426)
                     /gene="rplT"
                     /locus_tag="PLR72_02495"
     CDS             complement(76..426)
                     /gene="rplT"
                     /locus_tag="PLR72_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOK36114.1"
                     /translation="MPRSVNHVASRKRRKRILNLTKGYFLSRKNVWTVAKNTWEKGLQ
                     YAYRDRKTKKRNFRSLWIQRINAAARLEGMSYSKLMGALHKANIEINRKVLADLAMNH
                     PEAFKAIVEKAKNA"
     gene            complement(520..717)
                     /gene="rpmI"
                     /locus_tag="PLR72_02500"
     CDS             complement(520..717)
                     /gene="rpmI"
                     /locus_tag="PLR72_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOK36115.1"
                     /translation="MSKTKTNSGAKKRFSITGSGKIKRRHAFKSHLLRKKTKKQKRNL
                     THSALVDKTNLKSVKMLLGLK"
     gene            complement(740..1285)
                     /gene="infC"
                     /locus_tag="PLR72_02505"
     CDS             complement(740..1285)
                     /gene="infC"
                     /locus_tag="PLR72_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOK36116.1"
                     /translation="MTLRHQSREAGEYPHRINEEIKGVTQVRLVGDNVQQGIYSIEEA
                     LKIADEQNLDLVEISPKADPPVCKIIDYQKFLYQLKKREKEQKAKTARVVVKEIRFGP
                     QTDDHDYNFKLKHAQEFLKDGCKVKAYVFFKGRSILFKEQGEVLLLRFANDLEDYAKV
                     EHLPELEGKRMTIVLSPKKIK"
     gene            1647..2219
                     /locus_tag="PLR72_02510"
     CDS             1647..2219
                     /locus_tag="PLR72_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOK36117.1"
                     /translation="MKTFDLKGTKRTELGKKASKVIRKANEVPCVLYGGKENVFFTVS
                     NKEIQKLIYSPDVFVINLDIDKQKFTSIIQEIQFHPVTDAVLHIDFLEINEKKPVVVK
                     LPVKLEGLAEGVKAGGKLSLELRYLKVKGIYTKLPENITIDVTSLGLGKSIQIGSLSF
                     KDFEILNAKTAVVAQVKATRAAREAAAPGK"
     gene            2349..2930
                     /gene="pth"
                     /locus_tag="PLR72_02515"
     CDS             2349..2930
                     /gene="pth"
                     /locus_tag="PLR72_02515"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOK36118.1"
                     /translation="MKYLIVGLGNIGPEYENTRHNIGFTILDAFAKASNVFFTDKRYG
                     AICEIKLKGHTLILLKPSTFVNRSGLAVRYWMEKEKIEVPNLLVIVDDISLPFGTLRL
                     KPKGADAGHNGLKSIQEILGHNQYARLRVGIGNNYPRGKQVEYVLGHWTEEEANVLPK
                     LVEQAIEMIKSFCLAGIESTMSQFNNKQFLTAQ"
     gene            2927..3355
                     /locus_tag="PLR72_02520"
     CDS             2927..3355
                     /locus_tag="PLR72_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOK36119.1"
                     /translation="MKSEIRIDKWLWAVRLFKTRTLAAEACKKGKVLIQNVPVKPSHL
                     VKVGDVINIKRNPVLYSYKILKLAENRMNSKLVPDFMTDVTTPDQRELIELGKISQKM
                     GRKPGSGRPTKKERRELDNFIEEPTFWDEEDWDKEENEGK"
     gene            3426..3971
                     /locus_tag="PLR72_02525"
     CDS             3426..3971
                     /locus_tag="PLR72_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HOK36120.1"
                     /translation="MFIAQQLKNENICEYLLYMWQIEDVIRAFHLDMDAINSHIIASY
                     PASEIDQKKMYEWYESLIDMMRRENVEEKGHLQLNKNIIIELNEFHQQLLTSGKDHFY
                     NEKFYSIFPYIIELRKKSTTNVSDIELCFNFLYGIMTLRLQKAEIIPATKEAQEKISQ
                     FMSQLAKDYRLYQEGELKFDE"
     gene            4129..5001
                     /gene="rfbA"
                     /locus_tag="PLR72_02530"
     CDS             4129..5001
                     /gene="rfbA"
                     /locus_tag="PLR72_02530"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOK36121.1"
                     /translation="MKGIVLAGGSGTRLYPITKSISKQIIPVYDKPMIYYPLSILMLA
                     GIREILIISTPQDIHLYERLLNDGSDFGLKIEYAVQPSPDGLAQAFIIGENFIGNENV
                     CMILGDNIFYGYDFSKQLKEAAQLEDGALIFGYYVNDPQRYGVAEFDSEGNVLSLEEK
                     PEHPKSNYAVTGLYFYSNDVVEKAKQLKPSKRGELEITDLNRLYLNENRLKLKIMGRG
                     MAWLDTGTHDSLLEASNFIATIENRQGLKIACLEEIAYRSGYIDREQLVKLAEPLNKN
                     HYGEYLLKIANESK"
     gene            5067..5615
                     /gene="rfbC"
                     /locus_tag="PLR72_02535"
     CDS             5067..5615
                     /gene="rfbC"
                     /locus_tag="PLR72_02535"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOK36122.1"
                     /translation="MEIIKTPIPDLLVIKPQIFEDSRGYFYEVYNEKRYNEVGITYHF
                     CQDNQSKSTYGVLRGLHYQLSPASQSKLVYVIEGKVWDVAVDLRSTSPTFKQWYGIEL
                     SAENHLQFLIPKGFAHGYAVLSEMAIFAYKCDEFYNPSLERGIIYNDPILAIDWRIPA
                     EKMIISEKDSKHPFFDHAEFNF"
     gene            5626..6480
                     /gene="rfbD"
                     /locus_tag="PLR72_02540"
     CDS             5626..6480
                     /gene="rfbD"
                     /locus_tag="PLR72_02540"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOK36123.1"
                     /translation="MKTILVTGSHGQLGSEIQKISDHFSVFQYIYTDIEELDITDKKA
                     LEDFVIKNKAHFLVNCAAYTNVDKAEEEVDLCYKINAEAVKNIGEVAAEQHLKVVHIS
                     TDYVFDGKNFKPYVEEDLPSPVSVYGKSKLAGEEALLKNCQQAMIIRTSWLYSSFGNN
                     FVKKMLSLGKERQELNVVFDQIGTPTYAADLARTILIILQQQSFQPGIYHFSNEGVCS
                     WYDFAKAIFRIKNMECSIQPIESKEFPTKAQRPFYSVLNKAKIKNTFNLCIPHWEESL
                     EKCLSLLP"
     gene            complement(6573..6646)
                     /locus_tag="PLR72_02545"
     tRNA            complement(6573..6646)
                     /locus_tag="PLR72_02545"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6610..6612),aa:Asp,seq:gtc)
     gene            6997..7881
                     /gene="folD"
                     /locus_tag="PLR72_02550"
     CDS             6997..7881
                     /gene="folD"
                     /locus_tag="PLR72_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOK36124.1"
                     /translation="MYTLLDGKLISEQIKKEIAEEVRQMIAAGKKRPHLAVIIVGHDG
                     GSEAYVAGKIKDCEICGFKSTLIRFEDDVTEKRLLEEVQHLNEDADVDGFIVQLPLPK
                     HISEQKIIEAIDHKKDVDGFHPINVGRLCIGLPCYVSATPMGIMELLKRYQIETSGKN
                     CVVIGRSNIVGKPIAMLMMQKKYPGDATVTVCHSRTKNLPEICRQADIIVAALGQPEF
                     LKGDMVKEGVIVIDVGTTRVPSTETRSGYKLKGDVAFNEVAPKASYITPVPGGVGLMT
                     RVSLMKNTLLAAKREIYY"
     gene            7921..8649
                     /locus_tag="PLR72_02555"
     CDS             7921..8649
                     /locus_tag="PLR72_02555"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOK36125.1"
                     /translation="MNVFYVPEPTLRNTLSEEESQHAVKVLRLKEGEEIVLIDGKGGY
                     YKAKITFPDAKHCEFEIIETIPDFGKLNYYLHIAIAPTKNMERFEWFVEKAIEIGISE
                     ITPIICQFSERKVIKMERLEKIMIAAAKQSIRAYFPKLNPSCRFDDFLKKYQDSQQFI
                     AHCYSFIENSEQIQKLEKKKLKDVAQKSLRTIILIGPEGDFSKEEIKKAIAAGYIPIS
                     LGENRLRTETAGIIACHTIALINQ"
     gene            complement(8698..>9279)
                     /locus_tag="PLR72_02560"
     CDS             complement(8698..>9279)
                     /locus_tag="PLR72_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOK36126.1"
                     /translation="FELKEGIPEDQILDYCKKNRPVLVIMGTRGKKISSELIGSVTAE
                     VIEACVSPVLAIPAYVSLNSPAEIKRIAFLTNFDEKDLIAIDTTISLFGSENLEIYFI
                     HVSQKKEVWDEVMIGGIKEYFSKHYPELKTHYDLISSGDSLQLIDDYLAKNEIHLLAF
                     NSPRRNLIRRLFNPGLAYKMVLHTDTPLFVTHV"
     assembly_gap    9280..9379
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9833..12511)
                     /locus_tag="PLR72_02565"
     CDS             complement(9833..12511)
                     /locus_tag="PLR72_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36127.1"
                     /translation="MALRIEKIKFKSLGPIKNFDHDFKNVNLIYSKNEGGKSFLVEFI
                     IKSLFANIGQWKSIRDIDANYSKDDGIIISGINDGIITLNPKKKKTEKLDSIFNNNQG
                     MPSVLSKLLIVKSGEVEIAENTQAGINKNFIKDIFSNRPILNLIDDDKNISSTIKNST
                     INVEDNKINIAINKQGDGATYAELKDKINKINDKINDLSNKYNLSEIHRLEQELKQWK
                     DKKEQQIKAKCYKAYKLANEINNLQKQIDAIPINKINELQELIKNYKVLKTQIYNLEL
                     EINDLSKKTENLPKIEQNYKDQLNAKRYYAYTLHNQIKETENKLKFISNDEISELEKS
                     IDKYRENITKKEQLEKDIKNLEIKTQHYNWLSSLRTHQAEYLKSNITPNKVNMIYLVI
                     SLITIFTGFVTLFVTKNSIISGIFFIIGIGLGGFYIYKLQKHYKNLRQAEETNAVCEE
                     FKERFGTELNAVTIEEKYNSLTKDNAVLENKKNDLNEINININSLKTKINTLTNHIFG
                     QNIEENEWDNKIRDKKIEIKNLNNELLNSKTKLSQLNVDETDYVNVDNGIKYSKDELI
                     TLEKEIASMEPLFKEKNKKEEKSGILREERNEVLNEIKNKIKIIFNADLEESEWETKI
                     SEKIDEYTKLNNEKSKKEGELKGLGISESDYLADDPGIEYSPMTLQDIENTIEEIKQK
                     INDEDQKNKDLKNEICHLIGKDFTIEWNDLIDALYKTKDDVLNKLKDTEAKIIAGKIV
                     HDTINEIQKVEDENMLEAINSQKFKKLVKDLTLNYEELFFEDNEGKNFDEIKIYGDKG
                     EFYLKDLSTGTKEQVLFALRVTLAEKILSNKAFFILDDAFQHSDYNRRPKLIDQLFDL
                     SDAGWQIIYLTMDDHIRNLFLEKASHRNNFKQIMLG"
     gene            complement(12519..13583)
                     /locus_tag="PLR72_02570"
     CDS             complement(12519..13583)
                     /locus_tag="PLR72_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOK36128.1"
                     /translation="MKIGITADLHLKSINETPERYDAIEKIINYLNDSNINHLIIAGD
                     LFDKERYNYNDFDTFCIQHQDIIFYIIPGNHDAGIEQRYFSANNIKIITKPEILNFNS
                     LNFLFVPYDFKKSMDESIDSFISNRDNFNDLNSFVIVGHGDYMSNVKIENIYETGVYM
                     PLSSNIVNKYNPLQIFLGHIHKPSQLGKVWYSGSPYPLDINETGKKRFLTYDTQSNTV
                     DSIGIDNEIIYFIETILMLPVDDELAYINKKLEQMINNWQLSDVDINKVTLRLSLFGY
                     TKDINSLVNYIKNFIKNKGIKFYDANEVNISNLKIIKNEDNERNEIFKEVRKKIDSLP
                     NLDYADKDSILEKTMEIIFK"
BASE COUNT         4404 a         2086 c         2141 g         4940 t
ORIGIN      
        1 acctcaaaag ttttttataa ttttgaggtg taacttatgc aaaagactac tattttttac
       61 ctttgtagaa attaatcaag cattttttgc tttctcaaca attgccttaa aagcttcggg
      121 gtgattcata gctaaatcag ccaaaacttt tctatttatt tcgatattgg ctttatgcaa
      181 ggcacccatc aattttgaat aagacatacc ctcaagacga gccgcggcat taatgcgttg
      241 aatccataac gaacgaaaat ttcgtttttt ggttttacgg tcgcgataag catattgcaa
      301 gcctttttcc catgtatttt tggctactgt ccaaacattt ttcctactta aaaaataacc
      361 tttcgtcagg tttaaaattc tttttcttct tttacgtgaa gctacgtgat ttaccgatct
      421 tggcataata attaaattta cgaatgttag cgtcggactg ttaatacgaa ctttaggcta
      481 atgcattcaa ttaataatta aatttttaaa ataaactttt tattttaacc ccagcagcat
      541 ttttacactt ttcagatttg ttttatccac cagtgctgaa tgagttaaat tgcgtttttg
      601 tttctttgtt ttttttctta aaaggtggct tttaaaagca tgccttcttt ttatttttcc
      661 actcccggta atggaaaatc ttttctttgc accagaatta gtttttgttt ttgacatttt
      721 taaaaaaatt tacatctatt tattttatct ttttaggtga taatacaatg gtcattcttt
      781 tcccctccag ttcaggtaaa tgctcaactt tggcataatc ttccaaatca ttagcaaaac
      841 gtaataacaa aacttctccc tgttccttaa aaaggattga acgtccttta aaaaacacat
      901 acgcttttac tttacaaccg tcctttaaaa attcttgcgc atgctttaat ttaaaattat
      961 aatcgtgatc atcagtctgt ggtccaaaac gaatttcttt cacaacaacc cttgccgttt
     1021 tggctttttg ttctttttcc cgctttttta attgataaag aaatttctga taatcaataa
     1081 ttttacacac aggtggatca gctttgggcg atatttcaac caaatctaaa ttttgctcat
     1141 ctgcaatttt taaagcttct tcaatagaat aaatcccttg ctggacatta tcgcccacca
     1201 aacgtacttg agttactcct ttaatttctt catttatacg atggggatac tcacctgctt
     1261 ccctactttg atgccgaaga gttatttgtt tagctatatt tagtcctcct caattttaaa
     1321 atttaaacaa atcgattttt ttgaatttta aggttgcaaa tatagaattt ttttttcaat
     1381 tctctatgtt tttttaaaga atttatttac acttataaat ttttcagctt aaaaataagg
     1441 atatttatta actttatctg gctaccattt gtttaacaat ataaaaaaat actgtctctc
     1501 tgaattgaag gcaataaaaa attaagtacg tttgtacaac gtaaaatttt tatctacctt
     1561 tgcaaaaaga aagtgtgatg ggaaattaag caatctaatc actactaaaa ttagcttaaa
     1621 ttttgagtaa cacataaaat aaaaaaatga aaacatttga tttaaaagga acaaaaagaa
     1681 cagaattagg gaaaaaagca agtaaagtaa taagaaaagc taatgaagtc ccgtgcgttt
     1741 tgtatggcgg gaaagaaaat gtatttttta ctgtttccaa taaggaaatc caaaagttga
     1801 tctattcgcc agacgtcttt gttattaatt tggatattga taaacaaaaa tttacttcca
     1861 ttattcagga aattcaattt catcctgtta ctgatgcagt attgcatata gattttttgg
     1921 aaataaatga aaaaaagcca gttgtggtaa aacttcctgt aaaattggaa ggacttgctg
     1981 aaggtgtcaa agcgggcggt aaactgagtc ttgaacttcg ttacttgaaa gtaaaaggaa
     2041 tttatacgaa actacctgaa aatattacta ttgatgtcac ctcactgggg ctgggtaaat
     2101 ctattcagat tggttctctt tctttcaaag attttgaaat cttaaatgcc aaaactgctg
     2161 ttgttgctca agtgaaagcc acccgtgctg ctcgtgaagc tgcggctccc ggtaaataat
     2221 gaaatttttt acagaaagtt taagtatgtg acaaaatttt caattggaaa ttataaaatt
     2281 agcatttact taactttaat tttttattga tttttaaaaa agttacttca aaataaattt
     2341 ttcagggaat gaagtactta attgtagggt taggaaatat cggacctgaa tatgaaaata
     2401 cccgccacaa cataggtttt acaatattgg acgcctttgc gaaagcgtcc aatgtttttt
     2461 ttactgacaa acgatacgga gctatctgtg aaataaaact gaaaggtcat actttgattt
     2521 tattaaaacc ttctactttt gttaaccgaa gcggtcttgc tgtaagatac tggatggaaa
     2581 aagaaaaaat tgaggtacca aatttattgg taattgtaga tgatatttcc ttgccatttg
     2641 gcacacttcg tttaaagccc aaaggtgctg atgccggtca caatggctta aaaagcattc
     2701 aagaaatatt gggacacaat caatatgctc gactgcgtgt tggcataggg aataattatc
     2761 ctcgtggaaa acaagtagaa tatgtgctgg gtcattggac agaagaagaa gctaacgtat
     2821 taccaaaatt ggtagaacaa gccattgaaa tgattaaaag tttttgtttg gcgggaattg
     2881 aatcaaccat gtcacaattc aacaataaac agtttttaac cgctcaatga aatccgaaat
     2941 tcgcatagat aaatggctct gggcagtacg actgttcaaa acacgaacac tggcagccga
     3001 agcttgcaaa aaaggaaagg ttctgattca aaatgtgcct gtgaaaccct cgcatttggt
     3061 taaagtaggc gacgtaatca acatcaaaag aaatcctgtt ttgtattctt ataaaatact
     3121 taaactggct gaaaatcgga tgaattccaa attagttcct gattttatga ccgatgtcac
     3181 gacaccagat caacgagagt taattgaact tggaaaaatt tctcaaaaaa tgggtagaaa
     3241 acccggcagc ggcagaccta ccaaaaagga acgtcgtgaa ctggataatt ttattgaaga
     3301 acccactttc tgggatgaag aagattggga caaagaggaa aatgagggaa aatagaattt
     3361 aaattttcct cttaattcgt tagtgttttt taatttattc aggtttctca attgataaat
     3421 aaattatgtt tattgctcaa caattgaaaa atgaaaatat ctgcgaatac ttgctttata
     3481 tgtggcagat tgaagatgta attcgtgctt ttcatctgga tatggatgcc atcaacagcc
     3541 atattattgc ttcttatcca gcatcagaaa ttgatcaaaa aaaaatgtat gaatggtacg
     3601 aaagtctgat tgacatgatg aggcgggaaa atgtcgaaga aaaagggcat ttgcaattga
     3661 acaaaaacat tattatcgag ctgaatgaat ttcatcaaca gttgcttact tctgggaaag
     3721 atcattttta caatgaaaaa ttttattcca tttttcccta tatcatcgag ctgcgaaaaa
     3781 aatctactac caatgtcagt gacatcgaat tgtgttttaa ctttctgtat ggaattatga
     3841 ccttgcggtt acaaaaagca gaaatcatac cagccacaaa agaagcacag gaaaaaatca
     3901 gtcagttcat gagccagctc gcaaaggatt atcggcttta tcaagaaggg gaactgaaat
     3961 ttgatgagta aattgcaact ttttcaatat cttttttcat aatttattta taattttgaa
     4021 tgattcattt ttcactcatt ttttaaataa aaaaccattt atttattttg aaagttgaca
     4081 aaataatttt ataactttaa tgaattcatt tttaataatt tatatactat gaaaggcatt
     4141 gttctggcag gtggttctgg tacacgactt tatcctatta ccaaaagcat ttcaaaacaa
     4201 atcattcctg tttatgataa acccatgatt tattatcctt tatcaatcct tatgctggca
     4261 ggcattcgtg aaattcttat catttcaaca ccacaagata ttcatcttta cgaacgtttg
     4321 ttaaatgacg gaagtgattt tggattaaaa attgaatatg cagtgcaacc ttcacccgat
     4381 ggcttggcac aagcatttat tattggagaa aattttattg gcaacgaaaa tgtctgtatg
     4441 attttaggtg acaacatttt ttatgggtat gatttttcaa aacaactaaa agaagcagca
     4501 caattagaag atggagcgtt aattttcggt tattatgtta atgatcctca acgatatggc
     4561 gtggctgaat ttgatagtga aggaaatgta ttgagcctgg aggaaaaacc tgagcatcca
     4621 aaatcgaatt atgctgtcac aggcttatat ttttatagca atgatgttgt tgaaaaggca
     4681 aaacagttaa aaccttccaa acggggcgag ctggaaatca ctgacttaaa ccgtttgtat
     4741 ttgaatgaaa atagattgaa attaaaaatt atgggacgcg gaatggcgtg gctcgacacg
     4801 ggtacgcatg atagtttact ggaagcttcc aattttattg ctacgattga aaaccgtcag
     4861 ggtttgaaaa tagcctgtct ggaagaaatt gcttatcgca gtggctatat tgatcgagaa
     4921 cagttggtga agcttgccga accattaaac aaaaatcatt atggtgaata tcttcttaaa
     4981 attgccaatg aatcaaaata aatttatata atattctgta aataaaatat ttgcaatttt
     5041 taatctaaaa taaaataatt attctgatgg aaattatcaa aacaccaatt ccagatttac
     5101 tggtaatcaa accacaaatt tttgaggatt cacgaggcta tttttacgaa gtttacaacg
     5161 agaaaagata taatgaagtt ggaattactt atcatttttg tcaggataat caatccaaat
     5221 ctacttacgg tgttcttcgc gggttacatt atcagctttc accagcaagt caaagcaagt
     5281 tggtttatgt catcgaagga aaagtgtggg atgttgcagt agatttacgt tctacttcgc
     5341 caacttttaa acaatggtac gggatagaac tttccgccga aaatcatttg cagtttctaa
     5401 ttccaaaggg atttgcacat ggttatgctg tcctttctga aatggctatt tttgcttata
     5461 aatgtgatga attttataat ccatctttgg aacggggaat tatttacaac gaccctatac
     5521 tggctattga ttggagaatt ccagcagaaa aaatgattat ttcagaaaaa gatagtaaac
     5581 atcctttttt tgatcatgct gaattcaatt tttaaagatg aaaaaatgaa aactatttta
     5641 gttacgggca gtcatggaca attgggtagc gaaatacaaa aaatttctga tcacttttct
     5701 gtttttcaat atatttatac cgatatagag gaactggata ttaccgataa aaaggcactg
     5761 gaagacttcg ttataaaaaa taaagcccat tttttggtta attgtgctgc ttataccaac
     5821 gtagataaag cagaagagga agttgattta tgttataaaa ttaatgctga agcagtaaaa
     5881 aatattggag aagttgctgc agaacaacat ttaaaagtag tgcacatttc taccgattat
     5941 gtttttgatg gaaaaaattt taagccttac gtggaagaag atcttccttc gcctgtttcc
     6001 gtttatggaa aatcgaagtt ggctggtgaa gaagctttgt tgaaaaattg ccagcaagct
     6061 atgattattc gtacttcgtg gttgtattct tcttttggaa ataatttcgt caaaaaaatg
     6121 ttaagtctgg gaaaagaacg ccaggaatta aacgtggttt ttgaccagat tgggacgcct
     6181 acctatgcag cagatttagc aagaactatt ttaatcattt tacaacagca gtcttttcag
     6241 cctggaattt atcatttctc caacgaaggg gtttgctcgt ggtatgattt cgcaaaagct
     6301 atttttcgaa taaaaaatat ggaatgttca attcaaccaa ttgaaagtaa ggaatttcca
     6361 actaaagctc aacgaccttt ctatagcgtt ttaaataaag ccaaaatcaa gaatactttc
     6421 aatctctgca ttccccattg ggaagaaagt ttagaaaaat gcctttcgct tttaccatag
     6481 tgcaagtttt ttatctccaa aaattttttg gtatcaattt agataaaaag aaaagctcac
     6541 taccgaaaag taatgagctt tttaattttt tgcggtgcgg acgggacttg aacccgcgac
     6601 ccccggcgtg acaggccgat attctaacca actgaactac cgcaccagac tattttgttt
     6661 tggtagtgca aagataattc aaattttaat ttctacaata ctttccattc acttttttaa
     6721 gtgtaagaaa agggaaagaa gaaagaaagc acagtgatta aatttgaata ttttagcagc
     6781 aacttcaagt caaaatttta tcttttcctt cttatataga ttttaatcaa aagaattcct
     6841 cgaggcttac ctaggggtta ttcatttagg gtattccgta aatgctactt tgtttcaaca
     6901 tctttttatt tctgcaatta cacaaaatac tgtatatttg ttattgttta tgaataattc
     6961 atcttgtgga taaattttta aaaattaagt cgatttatgt atacactttt agatgggaaa
     7021 cttatttcgg aacaaataaa aaaagaaatt gctgaagaag ttcggcaaat gatagcagct
     7081 ggtaaaaaac gtcctcatct ggctgtaatc attgtaggac acgacggtgg tagtgaagcg
     7141 tatgtagctg ggaaaattaa agattgcgaa atttgcggat ttaagtcaac tttaattcga
     7201 tttgaggacg atgttactga aaaacgtttg ctggaagaag tgcagcattt aaacgaagat
     7261 gctgatgtgg atggctttat tgttcaatta cctttgccca aacatatttc agaacaaaaa
     7321 attatcgaag ctattgatca taaaaaagat gtggatggtt tccatcctat taatgtgggt
     7381 cgtttatgta tcggtttgcc ttgttatgtt tctgctacac cgatgggaat tatggaacta
     7441 ctaaaacgct atcaaattga aacgagtggt aaaaattgtg ttgttatcgg acgaagcaac
     7501 attgttggta aaccaattgc gatgttgatg atgcaaaaaa aatatccggg cgatgctact
     7561 gtaacagttt gtcatagccg tactaagaat ttgccagaaa tctgtcgcca agctgatatt
     7621 attgtcgctg ctttaggtca gccagaattt ttaaaaggtg acatggtgaa agaaggtgtg
     7681 atagtgattg acgtaggaac aacgcgagtt ccttctactg aaacgaggtc aggatacaag
     7741 ctcaaaggag atgtagcttt taacgaagtt gctcctaaag cttcttacat tacgcctgtt
     7801 cctggcgggg tagggttgat gacaagggtt tcgttaatga aaaatacact gttagccgct
     7861 aaaagagaaa tttattattg atttctggca ttttttccga aaaagtaaaa gaatgtaagt
     7921 atgaatgttt tttatgttcc tgaacccacg ttaagaaata ctctttccga agaagaatct
     7981 caacatgccg tcaaagtgtt acgtttaaag gaaggcgaag aaatcgttct gattgatggt
     8041 aaaggaggct attacaaagc aaaaattaca ttccctgatg ctaaacattg tgaatttgaa
     8101 attatagaaa cgatacctga ctttggcaaa ttaaattatt acttgcatat tgcgattgct
     8161 ccaaccaaaa atatggaacg ttttgagtgg tttgttgaga aagcgattga aattggcatt
     8221 agcgaaataa cacccatcat ttgtcaattt tcagaacgta aagttattaa aatggaacgc
     8281 ttggaaaaaa tcatgattgc tgctgccaaa caatccatca gagcttattt cccgaaatta
     8341 aatccctcgt gtcgttttga tgattttttg aagaaatatc aagattcaca gcagtttatt
     8401 gcacattgct attcttttat cgagaacagt gaacaaattc aaaaacttga aaaaaagaag
     8461 ctaaaagatg ttgctcaaaa atctttgaga accattattt taattggtcc agaaggtgat
     8521 ttcagcaaag aagaaattaa aaaagcgatt gccgcaggat acattccgat ttccctcggt
     8581 gaaaatcgtt tgcgtaccga aacagcaggt atcattgcct gccacactat tgccctcatc
     8641 aatcaataaa aagttttatt actttttttt cctactgatt ttcaaaaaaa cagaaagtta
     8701 cacgtgagta acaaataacg gcgtatcagt atgaagtacc attttataag ccaaacctgg
     8761 attgaataac cttctgatga gatttcgacg cggtgaatta aaagcaagaa gatgaatttc
     8821 attttttgcc agataatcat caatcaattg taaagaatca cctgagctta ttaaatcata
     8881 atgtgttttg agttcaggat aatgtttcga gaaatattcc tttatgcctc caatcattac
     8941 ctcgtcccat acttcttttt tttgagaaac atgaatgaag tatatttcca gattttcaga
     9001 tccaaacagg gaaatagtgg tatcgatagc aattaagtcc ttttcatcaa aatttgtcaa
     9061 aaaagcaatt cttttgattt ctgccggaga attcaatgat acgtaggcag gaatagccag
     9121 aacaggtgaa acgcaagctt caatgacttc tgctgtaacg ctaccaatca attcgctgga
     9181 aatttttttg cctcgtgttc ccataataac taaaacagga cggttttttt tacagtaatc
     9241 cagaatttga tcttcaggaa tgccttcttt caattcaaan nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnc atagtattaa atattttaaa ttcgtaattt acgatattaa
     9421 ttattttttc aaaactttct aatcgttttc aaatgtaaaa ttaatatatt ttctgaaata
     9481 aaggcaatct ttgctttttt aattttttct atgaaaaatg acgccgtaaa aatttggagg
     9541 ttgagaataa aaatccacat taatttattc atgatgtatt gataaaaaaa atcaattcac
     9601 aaaagtaaat tattgatttt atttttaaat taccgttata ttaagaaaaa tgggacgact
     9661 ttttgacatt gaaagtgaga aattcttatt cattagggga tgaacaaaag agaaatagtt
     9721 tagtctcttc aaaacaagtt gtccactatc ttgatagacg gatcaaggtg ttgaatgttt
     9781 ctgaataaag taaaaacaat ttttcaattg caaacaaact aatttggatg atttatccaa
     9841 gcatgatctg tttaaaatta ttccgatgcg aggctttttc gaggaacaaa tttctaatgt
     9901 ggtcatccat cgttaaatag ataatctgcc aaccggcgtc cgataaatcg aaaagttgat
     9961 cgataagttt aggtcttcta ttgtagtcgc tgtgctggaa agcatcatca agaataaaaa
    10021 atgctttgtt gctaaggatt ttttctgcca aggtcactcg cagagcgaaa agtacttgct
    10081 ctttggtgcc agtgctgaga tcttttaaat aaaactctcc tttatctccg taaatcttta
    10141 tttcatcaaa atttttacct tcattgtctt caaaaaataa ttcttcgtaa tttaaagtta
    10201 gatctttcac tagtttttta aatttttggg aattgatggc ttccagcatg ttttcatctt
    10261 ccactttttg aatttcattt atagtatcat gtacaatttt accagctatt attttggctt
    10321 ctgtatcttt caatttattt aaaacatcat ctttagtctt atacaatgca tctattaggt
    10381 cgttccattc tattgtaaaa tcttttccta tcaagtggca tatctcattt ttcaaatctt
    10441 tattcttttg atcttcatca ttgatttttt gttttatttc ttcaattgtg ttttctatat
    10501 cttgcagtgt cattggagaa tattctatac cagggtcatc ggctaaataa tcactttcag
    10561 atatacccag tcctttaagt tcaccttcct tttttgattt ttcattattt aattttgtat
    10621 attcatcaat tttttcagaa atttttgttt cccattcaga ttcttctaaa tctgcattga
    10681 aaattatttt tattttattt tttatttcat ttaaaacttc atttctttcc tctcttaata
    10741 ttcctgattt ctcctctttt ttgttttttt ctttaaatag aggttccatg cttgctattt
    10801 ctttttcaag agtaattaat tcatctttac tatatttaat gccattatcc acatttacgt
    10861 aatcagtttc atcaacatta agctgagaaa gtttagtttt tgaatttagt aattcattat
    10921 ttaaattttt aatttctatt tttttatctc ttatcttatt atcccactca ttttcttcaa
    10981 tgttttgtcc aaatatatga ttagtcaagg tatttatttt agtttttaag ctattaatat
    11041 ttatatttat ttcattcaaa tcattttttt tattttcaag tactgcatta tcttttgtta
    11101 agctattgta tttttcttca atagttacgg catttagttc tgttccaaat ctttctttaa
    11161 attcttcaca tacagcattt gtttcttctg cctgtcttaa atttttatag tgtttttgaa
    11221 gtttatatat ataaaaacca cccaagccaa tgccaatgat aaaaaatatt ccactaatta
    11281 ttgaattttt tgtaacaaat aaagtcacaa aaccagtaaa aattgtaatt aatgaaatta
    11341 ccaaataaat catattaact ttatttggtg taatgtttga ttttaaatat tcagcttgat
    11401 gcgttcttaa acttgataac caattataat gttgagtttt tatttccaaa tttttaatat
    11461 ctttttctaa ttgttctttt ttagttatgt tttctcggta tttatctatg cttttttcta
    11521 attcactaat ttcatcatta gaaataaatt ttaatttatt ttcagtttct tttatttgat
    11581 tatgcaatgt ataggcataa tatcttttag cgttcaattg atctttataa ttttgctcta
    11641 ttttaggtag attttctgtt tttttagaca aatcattaat ttctaattct aaattataaa
    11701 tttgtgtttt tagaacttta taatttttta taagttcttg caattcattt attttattaa
    11761 tggggattgc atcgatttgc ttttgaagat tatttatttc atttgccaat ttatatgctt
    11821 tatagcattt agctttgatt tgttgttctt ttttatcttt ccattgtttt aattcttgtt
    11881 ctaatctgtg aatttcgctt aaattatatt tattacttaa atcatttatt ttgtcgttaa
    11941 ttttattaat tttatctttt aattctgcat aggtggcacc atcgccttgc ttatttattg
    12001 caatatttat tttattatct tcaacattaa tggtagaatt ttttatcgtt gaagaaatgt
    12061 ttttatcatc atcaattaga ttaaggattg gcctattaga aaaaatgtct tttataaaat
    12121 ttttatttat gcctgcttgt gtgttttctg ctatttcaac ctcaccactt ttaactatta
    12181 gtaatttaga aaggacggat ggcattcctt gattgttatt gaaaatagaa tcaagtttct
    12241 ccgtcttctt ttttttagga ttcaacgtga ttattccgtc attgataccg gatattatta
    12301 ttccatcgtc tttactataa ttagcatcaa tgtctcgtat acttttccat tgaccaatat
    12361 ttgcaaataa agatttgatg ataaattcta ccaaaaatga tttaccgcct tcatttttac
    12421 tgtaaattaa gttcacattt ttaaaatcgt gatcgaaatt cttgataggg ccaagacttt
    12481 taaactttat tttttctatt cttaatgcca tattttcact atttaaaaat tatttccata
    12541 gttttttcta atatactatc tttatcagca taatctaaat ttggtaacga gtctattttc
    12601 tttcttactt ctttaaatat ttcatttctt tcgttatctt cattttttat aatcttcaaa
    12661 tttgaaatat ttacttcatt ggcatcataa aactttattc ctttattttt tataaaattt
    12721 ttaatataat tgactaatga attaatatct ttggtatatc caaataaact caatcttaat
    12781 gtaactttat taatatcaac atcgcttaac tgccaattat ttatcatttg ttcgagtttt
    12841 ttatttatat atgctaattc atcatctact gggagcatta aaattgtttc aataaaatag
    12901 attatctcat tatcaatgcc tattgaatca acggtattgc tttgagtatc gtaagtgagg
    12961 aatctttttt tacccgtttc gttgatgtca agtggatatg gtgagcctga ataccaaact
    13021 ttccccaatt gagaaggttt gtgtatatga ccaagaaata tttgcaatgg attgtatttg
    13081 ttaacgatgt tgcttgagag tggcatataa acaccagttt catagatgtt ttcaatttta
    13141 acatttgaca tataatcacc atgacctact ataacaaaac tatttagatc attgaaatta
    13201 tccctatttg aaataaaact atcaatagat tcgtccatag attttttaaa atcataaggt
    13261 acgaaaagga aattcaaaga attaaaattt aagatttcgg gtttggtaat aatttttata
    13321 ttattggcac taaaatatct ctgttctatc ccagcgtcgt gattacccgg tatgatataa
    13381 aaaataatat cttgatgttg tatgcaaaat gtgtcaaaat cattataatt gtatctttct
    13441 ttatcaaaca aatctccagc aatgattaga tgattaatgt tactatcgtt taaatagttt
    13501 ataatttttt ctattgcatc gtatctttct ggagtttcat ttattgattt caaatgtaga
    13561 tctgctgtta tacctatttt cataatttta aaattaatga ggttattgtt cgaatgtttt
    13621 ttattgtttc attcgattat atttcacttt atcacgaggg aaagaacaac a
//
LOCUS       DAOSAS010000032        13519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3084_length_13519_cov_12.848633, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000032 DAOSAS010000000
VERSION     DAOSAS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13519
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3084_length_13519_cov_12.848633"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..790)
                     /gene="cmk"
                     /locus_tag="PLR72_02575"
     CDS             complement(104..790)
                     /gene="cmk"
                     /locus_tag="PLR72_02575"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOK36129.1"
                     /translation="MKKIIVAIDGPSSCGKSTIAKELARDVGYVYVDTGAMYRAVALY
                     CLQNGWLKDSSLDEEALKKNIKSLKIEFKTNEKGETETYLNGRNVEKEIRSLEVANAA
                     SQISMLDFVRKEMVCQQQEMGKEKGIVMDGRDIGTVVFPEAELKIFLTATPEIRAQRR
                     MLEMLERGEKVTFEEVLKNIRERDERDSHRLESPLKKAEDAIVIDNSNLTKEQQRELV
                     KTLFLEKVLS"
     gene            complement(869..2137)
                     /gene="purD"
                     /locus_tag="PLR72_02580"
     CDS             complement(869..2137)
                     /gene="purD"
                     /locus_tag="PLR72_02580"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOK36130.1"
                     /translation="MNILILGSGGREHAIAWKISQSPNVTCLYIAPGNAGTSSIGINL
                     PIEVNDFESIKEAVKNFQINYVIVGPEDPLVKGIVDYFQQDEDLKKVYIIGPDKRAAQ
                     LEGSKDFAKNFMFRHGIPTAKYLSVTATNLEKGIDFLKKMCPPYVLKADGLAAGKGVE
                     IISELEKAQEELRIMLEGKFGTASQKVVIEEFLSGIECSVFVVTDGENYKILPEAKDY
                     KRIGEGDTGLNTGGMGAISPVSFADEAFMQKVEKKIIKPTIQGLLEEGIVYKGFIFFG
                     LMKVDSEPYVIEYNVRLGDPETEAILPRISSDLVDLLEGITHCNLSDKKLAIDPRFVA
                     TVILASGGYPEKYEKGKVITGLEKVKDSIVFHSGTAFKDGKMVTNGGRVLAISSYGKT
                     KEEALELSYRNAEKIQFENKYYRKDIGFDL"
     gene            2818..3984
                     /locus_tag="PLR72_02585"
     CDS             2818..3984
                     /locus_tag="PLR72_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HOK36131.1"
                     /translation="MGGIFMKLWEKSFNQCLILLCFFIFFSFLTSFASKNVLVYTPYT
                     QVFVSPGESVDYTIDVINNGESIFNQEITINGIPRNWNYILRLDGYKVGRVAVLPKEK
                     KSLSLKVEVPFQVNKGTYAFRVAAGDSASLTLRITVTEKGSSETEFTTDQANMQGNAT
                     SNFSFRTTLKNRTAEKQHYALIADAPRGWTVTFNADYKQVTSVEMEPNTTKDITIDIK
                     PTAKVKSGTYKIPVRAMTGSTSADLELEVVITGNYAIELTTPTGLVSTNITAGKEKKV
                     TLVVKNTGSADLSGIEMKATTPSKWEVTFEPSKIDKLLPGEQATVNATLHADKKAIPG
                     DYIVNINASTPEATADISFRVMVKTSMVWGWIGIIIILAALGGVFYLFRKFGRR"
     gene            3987..4742
                     /locus_tag="PLR72_02590"
     CDS             3987..4742
                     /locus_tag="PLR72_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK36132.1"
                     /translation="MSEIMVELVNLTKKYKSFTAVDQINLSVKKGEIFGFLGPNGAGK
                     STTILMMLGLTEPTEGKVEVCGINPTRYPIEVKRKVGYLPEDVGFYDNRTGLYNLLYT
                     AMLNRIPENEARNKALELFQLVGLEGVENKKTGQYSRGMRQRLGLADVLIKNPEMIIL
                     DEPTSGIDPQGVKSFLDLILQLRNEQGLTILFSSHNLHQVEQVCDAVGIFSKGKIIAS
                     GNIPDLSRQVFNDETHSLEDVYNQFFEGGNQDE"
     gene            4810..5769
                     /locus_tag="PLR72_02595"
     CDS             4810..5769
                     /locus_tag="PLR72_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOK36133.1"
                     /translation="MKKTKVKHPFWVMVSKEISDQVTSWRFIVMILLIVLTCMGSLYT
                     ALGQFTKSSASVGSDSFFFLKLFTLSDGTLPPFIIFISFLAPLLGISLGFDAINSEYN
                     RGTLSRILSQPIYRDYIINAKFMAALMVVSFLFFTLGLLVIGCGLIALGIPPSSDELF
                     RILFYLIISIVYVAFWLNLSILFSVTFRQPATSALAGIAVWLFFSVFYNMIVELLAKG
                     LAPRGMVTEQQIVNFQNGILNLMRLNPSQLFSDANITLLMPSVRSLGALTMSQVEGAL
                     PTPLPLGQSLLMVWPQVTGLIAGTILLFAISYYLFMRQEIRSR"
     gene            5954..6388
                     /locus_tag="PLR72_02600"
                     /pseudo
     CDS             5954..6388
                     /locus_tag="PLR72_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            6491..6631
                     /locus_tag="PLR72_02605"
     CDS             6491..6631
                     /locus_tag="PLR72_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOK36134.1"
                     /translation="MNKKFKCLVCGYIYDEAQEKVKFEDLPDDWVCPLCGTAKSEFIE
                     IK"
     gene            6706..7284
                     /locus_tag="PLR72_02610"
     CDS             6706..7284
                     /locus_tag="PLR72_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOK36135.1"
                     /translation="MEKSLKGTKTEQNLLKAFAGESQARNRYEFFASVAKKEGYEQIA
                     AIFMETASQEKEHAKRFFKFLEGGMVEITASYPAGVIGDTKQNLKAAAEGEYDEWSNL
                     YPTFAQVAEEEGFSEIAVAFKMVAKVEVQHERRYLKLLQNISEDKVFKKDGKVWWKCR
                     NCGYIYESAEALETCPVCLHPRAFMELWQENY"
     gene            complement(7352..8101)
                     /locus_tag="PLR72_02615"
     CDS             complement(7352..8101)
                     /locus_tag="PLR72_02615"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOK36136.1"
                     /translation="MTLQEQFEKQGNFLFKYRSYLPLIIIVAGLLVYIFPLLKNPNPS
                     FASYTILVFVSLAISFIGFAIRVYAVGHTPANTSGRNTAEQIADELNTTGIYSIVRHP
                     LYLGNFFMWLGATLLTANGWFVVIFVLVYWLYYERIMYAEEQFLQRKFGETFLRWAEK
                     TPTFIPAFHRFVSSRYPFSWKKAMKKEKNGLVAIFILLFVFYNIRYSFKNHHFSIDLN
                     WIFWVTLLTVLLYLVLKFLKKKMHVLDEKGR"
     gene            complement(8198..8860)
                     /gene="ung"
                     /locus_tag="PLR72_02620"
     CDS             complement(8198..8860)
                     /gene="ung"
                     /locus_tag="PLR72_02620"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOK36137.1"
                     /translation="MNVKIEPQWQTILQPEFDKDYFVRLTDFVRNEYKTRRIFPPGSL
                     IFNAFNLCPYDKVKVVILGQDPYPNEGQAHGLCFSVNENVEFPPSLMNIFKEIQAEFG
                     KPIPKNGNLTRWAEQGVLLLNAILTVQAHLPTSHQGKGWEIFTDAVIRKLATEKEHLV
                     FMLWGNYAQQKGTFIDPNRHLVLKSSHPSPLSANRGFFGNQHFILANQYLKAHGEKEI
                     DW"
     gene            complement(8872..10044)
                     /locus_tag="PLR72_02625"
     CDS             complement(8872..10044)
                     /locus_tag="PLR72_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOK36138.1"
                     /translation="MKYNFDEIIDRRNTNAVKIEACPSLFGTNDVLPLWVADMDFRTP
                     DFILETIRRRCKHPILGYTIMPKEYFPSIIYWLNQIHQFEVKEEWLGFLPGVVTGLAL
                     AVKQFSSERDEVIVQPPVYYPFMEVVKSNHRKLVYNPLQERNGKFEMDFEDLESKISS
                     RTKLLLLCNPQNPGGRIWDRTTLQTLAEICERHHILIVSDEIHGDMALARNQHVPFAS
                     VSNKAAQISITLMSPSKIFNIPSLQAAYYIIPNEEIRNLFKDFLNQLRITDGNIFAYI
                     ATIAAYTQGAEWRRQMLQYVNKNVEYVIHFLKEELPLIEPMKPEASFLIWLNCEKMGM
                     TDEELFQFFIQKAGLGLNKGKIFGPGGEQHFRLNVACPRNVLQQAMKQLKSAFESF"
     assembly_gap    10173..10272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10273..11855)
                     /locus_tag="PLR72_02630"
     CDS             complement(<10273..11855)
                     /locus_tag="PLR72_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOK36139.1"
                     /translation="MKFFVRNFWLLLIFNVLATYGAAQNFTSKPPRLVVGIMIDGLQQ
                     RHIDQLWNRFDSYGFKQIVEKGAICNNIYYNLLSAGNASDIATVMTGTTPYYHGVTGN
                     HYFSRKNKEVQSIIYDENELGIGTKLTVSAHHLLSSTVTDELMLNFSHQSKTYAVAIN
                     PEEALMLAGHTANSAVWISDETMKWITTTYYSDGLAASADEMNVNDQIRKYASMIWEP
                     MYSPNTYLYPPQGENKKTSFQYKPDSKRKNSSLLLLKNTPTANAVVADLAYRIINQEK
                     LGTDDYPDFLLLQFTVKTPYEKTSVINSAEKEDIYLQLDKDIQYLLQKIDSKIGMSQV
                     LVYMFGNQTNFHTPIELGNNKIPAGYFNGDRSMALLNTYLMAIYGQEKWVEGYYGKNI
                     FLNRQKIEEKKLNLYDIQQTVADFMLEFEGVQAAFTATQVLNANSQTNDQIIPIRNSF
                     HKNTSGDVILTLLPGWIELNENNQPVGESNAVISYTPVYFYGWNIKSQKINTFYPVTS
                     IAPTLSKILNIPEPNACVGT"
     gene            complement(11904..13025)
                     /gene="hisB"
                     /locus_tag="PLR72_02635"
     CDS             complement(11904..13025)
                     /gene="hisB"
                     /locus_tag="PLR72_02635"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HOK36140.1"
                     /translation="MKKRVLFIDRDGTILVEPSETYQVDTLEQLEFLPGVIRNLYFIR
                     QKLDFEWALVSNQDGLGTLAYPQKNFDVVQQKFLQILKNEDITFDKIFIDKSLPEESL
                     PTRKPGIGMLTEYFSEDYDLQNSFVIGDRYTDVELAKNLGCKAIFLNNDEKILEKKKL
                     TEYCVLQTSSWDKIAEFLFAGERTIVVQRTTKETDVYVYLNLDGEGKSSISTGLHFFD
                     HLLEQISKHSGCDLTIQVKGDLEVDEHHTIEDTALALGEAFAKTLSDKRGIERYGFCL
                     PMDDSLSMVAIDFGGRPWLVFDAHFQREYIGDFPTEMILHFFKSFSDAAKMNLYIKTE
                     GENEHHKLESIFKALAKAIKMAVKRDIYHFDLPSTKDAL"
     gene            13206..>13519
                     /locus_tag="PLR72_02640"
     CDS             13206..>13519
                     /locus_tag="PLR72_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOK36141.1"
                     /translation="MDWIKSILVDNGVAEIILVYSLVITAGILLGKIKFFGVSLGVTF
                     VLFVGLFAGHLGFKGNPEVIEFIKEFGLILFVYSIGLQVGPSFFSSFKKGGIQLNLLA
                     TS"
BASE COUNT         4509 a         2320 c         2110 g         4480 t
ORIGIN      
        1 aaaacccgat tttttatcta tttctatttt caagagaaaa agtttatata aaatttttca
       61 gtaaatgaca agaaaaaagt atgagaagaa atttcgttat tttttatgat aacacctttt
      121 ctaaaaataa agtttttacc aattctcgtt gttgctcctt tgtaagatta gaattgtcaa
      181 ttacaatagc atcttctgct ttttttaaag gactttccaa tcgatgtgaa tctctttcat
      241 cacgttcacg gatatttttc aaaacttctt caaaagttac cttctcacct ctttccagca
      301 tttctagcat gcgcctttga gcacggattt caggtgtggc agtcaaaaaa atctttaatt
      361 ctgcttcggg aaaaacaacc gttcctatat cacgaccatc catcacaatt cctttctctt
      421 tccccatttc ttgctgctga caaaccattt ccttccgaac aaaatctaac atactgattt
      481 gacttgccgc atttgctact tctaacgaac gaatttcttt ttcaacattc ctgccattca
      541 aataagtttc tgtttctccc ttttcattgg ttttaaattc aattttgaga gattttatat
      601 ttttctttaa agcttcttcg tcaagtgaag aatcctttaa ccagccattt tgtaaacaat
      661 ataaagctac tgctcgatac atcgctcctg tatcgacata aacatatccc acatcacgag
      721 ccaattcttt ggcaatggta ctttttccgc acgatgaagg tccatcaata gcaacaatta
      781 tttttttcat gattgatttc gttcattatt ttgttaaaaa gagaaaaagg aggagattat
      841 caggaaatta tgtgtattat tcccattttt aaagatcaaa acctatatct tttcgataat
      901 atttattttc aaactgaatc ttttcagcat ttcgataaga taattccaat gcttcttctt
      961 ttgttttgcc ataagagcta attgccaaca ctcgtccacc gttagttacc attttaccat
     1021 ctttaaaagc agtaccagaa tgaaagacaa tactatcttt tactttttct aaaccagtga
     1081 ttacctttcc tttctcatat ttttcaggat aaccacctga agccaatata actgttgcta
     1141 caaagcgagg atcgatcgcc agctttttgt cactcaaatt acaatgggta attccttcca
     1201 acaaatctac cagatcagaa gaaatacgag gcaaaatggc ttccgtttca ggatcaccca
     1261 accgaacatt gtattcaatg acgtaaggtt cactgtccac tttcatcaat ccaaaaaata
     1321 taaaaccttt ataaacaatt ccttcctcaa gcaacccttg aatagttggc ttaataatct
     1381 ttttttctac tttctgcata aacgcttcat cagcaaacga aactggagaa attgctccca
     1441 taccacctgt atttaaaccc gtatcgcctt ctccaatgcg cttgtaatct ttggcttctg
     1501 gtaaaatttt ataattttct ccatcagtga ctacaaaaac agaacattct attccagaaa
     1561 ggaattcttc aattactacc ttttgacttg ctgttccaaa ttttccttcg agcattattc
     1621 tcaattcttc ctgagctttt tcaagttcag aaattatttc tacacctttt cctgctgcca
     1681 agccatcagc ttttaaaaca taaggcggac acattttttt taagaaatct attccttttt
     1741 ccagatttgt ggcggtaaca cttaaatatt ttgcagtggg aataccatga cgaaacataa
     1801 aattttttgc aaaatctttg cttccttcca attgagcagc ccttttatca ggaccaatta
     1861 tataaacttt tttcaaatct tcatcctgtt gaaaataatc aacaattcct ttaacaaggg
     1921 gatcttcagg tcctacaatc acatagttaa tttggaaatt ctttacagct tcttttattg
     1981 attcaaaatc atttacttcg ataggcaaat tgattcctat agaggaagtt ccagcatttc
     2041 ctggagcaat atacagacaa gtcacatttg gactttgcga aattttccaa gcaatggcat
     2101 gttcacgtcc acctgaacca aggatgagaa tattcataac aaattatttt tcgtacaaaa
     2161 ataatttaat ttttagagtt gaacaaaata acaggaaggt tgaaaaagtt gatgagtaga
     2221 aaagaaagta aaaaaacaac aaaaagttct acaaaaaaga aaaggtagtc acgcttgact
     2281 accaatcttt tttgttaacc ttaaaatcta atactatgaa aaaaattacg atgcaaatgt
     2341 atagcttttt taaataacta ccaaacttct ccaaataaat ttaaataaaa atatgttata
     2401 taacatattt taaataatgt cctcttgtta tgagtatcat tgagtgaaat aatttttttc
     2461 tggtttgatt ttcaaagtta taaaaaaatc ttcacttaat cataaaataa attttaaata
     2521 ttttaagaga aaataatcag gaaaaatttt tattttagta aagaaattcc ttaaaaattg
     2581 actatcctcg ccacaagcag cgaggtatta cgccaatttt atggcttcgc cactggaatt
     2641 tctaaaaaaa taaaaacaaa agctactccg acgcaagcat cggggaattc ttacgattaa
     2701 aaagaaatac aacttatcct gctttaaaat acgattttaa ttttttaaaa aaattaacat
     2761 ttataaataa agtaatttac taaacttgca accgtattaa ataaacatac ttaaagaatg
     2821 ggaggaattt ttatgaaatt atgggaaaaa tcttttaatc aatgcttaat tcttttatgt
     2881 ttttttattt tcttttcatt tttaacttcc tttgcttcta aaaatgtttt agtgtatact
     2941 ccttatacac aggtatttgt ttcgccagga gaatcagttg attatacaat tgatgttatt
     3001 aacaatggtg aatccatttt taatcaagaa ataactatta atggtattcc aaggaattgg
     3061 aactacatat tgagactgga cggatataaa gtaggaagag ttgctgttct tccgaaagaa
     3121 aaaaagagtc tttccttaaa agtggaagtt ccttttcagg taaataaagg gacctatgct
     3181 tttagagttg cagcaggtga ttctgcttct ttaaccttaa ggattacggt cactgaaaaa
     3241 ggttcaagcg agacagaatt taccacggat caagcaaata tgcagggaaa tgcaacatca
     3301 aatttttcat ttagaacaac gctcaaaaac aggacagccg aaaaacaaca ttatgccttg
     3361 atagccgacg ccccaagagg ttggactgtt acttttaatg ctgattataa gcaagttacc
     3421 tctgttgaaa tggaacccaa tactaccaaa gatattacca ttgatataaa acctactgca
     3481 aaagtaaaat caggaactta caaaatacca gtgcgtgcaa tgacaggttc tacttcggct
     3541 gacttagaac tggaagtggt aattacagga aattatgcca tagaactcac tactccaaca
     3601 ggtttggtaa gcacaaacat aacggctgga aaagaaaaga aagtaacact tgttgttaaa
     3661 aatacagggt ccgctgattt atctggaatt gaaatgaaag caacaactcc atctaaatgg
     3721 gaagtcactt ttgaaccatc aaaaatagac aaattacttc ctggtgaaca agctactgtc
     3781 aatgccactc tacatgcaga taaaaaagct attccgggtg attatattgt taatattaat
     3841 gccagtactc ctgaagctac tgctgatata tcatttagag taatggttaa aacttctatg
     3901 gtgtgggggt ggataggaat catcattata ttagcagctt taggtggcgt attttattta
     3961 ttcagaaaat tcggaaggag gtaactatgt ccgaaataat ggttgaattg gttaacttga
     4021 caaaaaaata taaatctttt acggctgttg atcagataaa tttatcggta aaaaaagggg
     4081 aaatatttgg ttttttaggt cctaatgggg caggaaaatc taccaccatc ttgatgatgt
     4141 taggattaac cgaaccaact gaaggaaaag ttgaagtttg tggtatcaac ccaacacgtt
     4201 atcctatcga agtaaaacga aaagtcggat atttacccga agatgtggga ttttacgata
     4261 acagaacagg actgtataac ctcctctaca cagctatgct taatcgaatt cccgaaaatg
     4321 aggctcgaaa taaggcatta gaactttttc aattagttgg cttggaaggt gtggaaaata
     4381 aaaaaacggg gcaatattcg cgtggaatgc gtcagcgact tggcttggca gatgtgctaa
     4441 taaaaaatcc agaaatgatt attctggatg aaccaacttc aggtattgac ccgcaaggtg
     4501 taaaatcatt cctggactta attttgcaat tacgtaacga gcaaggatta actattcttt
     4561 tttcttctca taatcttcat caggtggaac aagtttgtga tgcagttgga attttttcaa
     4621 aaggaaaaat aattgcttca ggcaatatcc cggatttgtc caggcaagtt ttcaatgatg
     4681 aaactcacag cttggaagat gtgtacaatc aattttttga aggagggaat caagatgaat
     4741 aatgcttttt cttctgcaga aaaactctct ggttattttc aacctacttt tgaaacaaac
     4801 ttgtcagtta tgaaaaaaac gaaagtaaaa catcctttct gggtaatggt ttcaaaggaa
     4861 atatcagatc aagtaacgag ctggcgattt attgtaatga ttttattaat tgtgttaacc
     4921 tgcatggggt cactttatac agctttgggg cagtttacaa aatcttctgc cagcgttggg
     4981 agcgacagtt tcttttttct aaaattattt actttatctg atggcacttt gccacctttt
     5041 atcattttta tcagttttct ggcgcctttg cttgggataa gtttaggatt tgatgctatt
     5101 aattcagaat acaacagagg aacgttaagt agaattttaa gccagcctat ttacagagat
     5161 tacatcatca atgcaaaatt tatggctgca ttgatggtag taagttttct tttctttacg
     5221 ctgggactgc tggtaattgg ttgtggattg attgctttag gaattcctcc ttcctccgat
     5281 gaattgtttc gaatattatt ttacttgata attagtattg tttatgtcgc cttctggctt
     5341 aatttatcca ttcttttttc agtaactttt agacaaccag ctacttctgc tttagcaggc
     5401 attgcagtgt ggcttttttt cagcgttttt tataatatga ttgttgagtt gcttgccaaa
     5461 ggtttggctc caagaggaat ggttacagaa caacaaatcg taaattttca aaatggaatt
     5521 ttaaatttaa tgcgactgaa tcccagccag ctttttagcg atgcaaatat tactcttctc
     5581 atgccatcgg taagaagtct gggagcatta acaatgtcac aagtggaggg tgctcttcct
     5641 acacccctgc ctcttggtca aagtttgctt atggtttggc cgcaagtaac cggacttatt
     5701 gcaggaacta ttcttttgtt tgcaataagt tattatctat ttatgcgtca agaaatcaga
     5761 tcaagataat taaatacaaa caaatgaaac ccattttatt gagaggatgt tttgaaaaaa
     5821 gcatcctttt tatttccaaa tgaaaaaata tttatgagaa tattgttcaa atgaaattaa
     5881 tttttaaatt tgtttgttag aatttcaaaa aagaatattt caaaaaataa aaataaaaac
     5941 tcgatttttt tcaatgaacg taacgacatt ttttaaaatt tcctatggtt tgtatattgt
     6001 aagttcagga aataaagaaa aaggtaatgg ttttatttcc aacacagtat ttcaggtaac
     6061 ttctgaacct ccacgattta cagtttgctg caataaagac aattatacct gtgaatttat
     6121 taatcaatat aaagttttct ctgtttcagt tctcgcgcag gaggcttcca gtgaaattat
     6181 cggcaaattt ggttacaaaa gtggtagagg aactgataaa atgagtggaa ataatattat
     6241 ttatggacaa acaggtgttc ccatcgtatt aaatgatagc attgcttttt tagaatgtaa
     6301 agtgatacaa acttttgatg tgggtactca tttgctgttt attggtgatg tagttgatgc
     6361 acaaataata gatgacactc agccgccatt aacttatgct tattaccgag aaataaaaaa
     6421 aggagtatca cctaaaaatg ctcctactta tatcaatcca actgatttat caacttctaa
     6481 aataaattca atgaacaaaa aatttaaatg tcttgtttgt ggttatattt atgatgaagc
     6541 ccaagaaaaa gttaaatttg aagatttacc tgacgattgg gtatgtccac tttgtggcac
     6601 agcaaaatct gaatttattg aaataaaatg aaacaactca aaaatattat caaataaatc
     6661 atgtccaatc aatttctatt gtaatacaat ttaattataa aaaatatgga aaaatcattg
     6721 aaaggtacaa aaactgagca aaacttattg aaagcctttg caggagaatc acaagctcgc
     6781 aatcgttatg aattctttgc cagcgtggcc aaaaaagaag gttacgaaca aattgcagct
     6841 atctttatgg aaactgctag tcaagaaaaa gaacacgcca aacgtttctt taaatttctg
     6901 gaaggcggta tggttgagat taccgcttct tatccggctg gcgtaattgg tgacaccaag
     6961 caaaacttga aagctgctgc tgaaggagaa tatgatgaat ggtcaaactt gtatccaact
     7021 tttgctcaag ttgctgaaga agaaggattt tctgaaattg cagttgcttt taaaatggta
     7081 gcaaaagtag aagtgcagca tgaacggcga tatcttaaac ttctacaaaa catttccgaa
     7141 gacaaagtct ttaaaaaaga tggaaaagtt tggtggaaat gcagaaattg tggctacatc
     7201 tatgaatcag cagaagcttt ggaaacttgt cctgtttgtc tgcatccaag agcatttatg
     7261 gaattatggc aggaaaatta ttgattatca taacattatt tcccaaaaat ttattaaacc
     7321 tctctgaaaa ttttttcaaa gaggtttatt tttatctacc cttttcatct aaaacgtgca
     7381 ttttcttttt cagaaatttt aaaaccagat aaagtaaaac ggtcaaaagt gttacccaaa
     7441 aaatccaatt caaatctatt gaaaaatgat gattcttaaa agaataccga atattataaa
     7501 aaacaaaaag taaaataaaa atagcaacta agccgttttt ttctttcttc atagcttttt
     7561 tccagctaaa aggataacgc gatgaaacaa atctatgaaa agcaggaata aaagtaggtg
     7621 ttttttctgc ccatcgaaga aatgtttcac caaatttgcg ttgtaaaaat tgttcctctg
     7681 catacataat tctttcataa taaagccaat aaaccaatac aaatattaca acaaaccatc
     7741 catttgctgt caataaagtt gcgcccaacc acataaaaaa atttcctaaa taaagtggat
     7801 gtcgaactat tgaataaatt ccggtggtat ttaattcatc tgcaatttgt tcagctgtat
     7861 ttcttccaga agtatttgcg ggagtatgtc ccactgcata aactcgaata gcaaaaccaa
     7921 taaatgaaat agccaaactt acaaatacta aaattgtata agatgcaaaa gaaggatttg
     7981 gatttttcaa taaggggaaa atataaacca ataaacctgc aactataatt atcaaaggca
     8041 aatagctgcg atatttgaac aaaaaatttc cttgtttttc aaattgctct tgtaaagtca
     8101 ttgaataaat aaattaaaag ttaaataatt taaagtcaaa tgcttattaa aaacgtacaa
     8161 ccgaacattt tattttttta aacagcataa gaatttttta ccaatcgatt tctttttctc
     8221 catgagcttt gagatactga ttggcaagta taaaatgttg attaccaaag aaacctctat
     8281 tggcagaaag aggtgaagga tggctcgatt ttaaaaccag atgacgattt ggatcaataa
     8341 atgtaccttt ttgctgagca taatttcccc ataacataaa aacgagatgt tccttttcag
     8401 tagctaattt ccgaataacc gcatcagtaa agatttccca gccttttcct tgatgagaag
     8461 ttggcaaatg agcttgaaca gttaaaatgg cattgagtaa taaaacgcct tgctcagccc
     8521 aacgagttaa attcccattt tttggaatcg gtttaccaaa ttctgcttga atttctttaa
     8581 aaatattcat caaagaaggt ggaaattcta cattttcatt cactgaaaaa cataaaccat
     8641 gagcctgccc ttcgttgggg taaggatctt gaccgagaat aaccactttt accttatcat
     8701 aagggcaaag attaaaagca ttgaatatca aagaaccagg tggaaaaatc cgtctcgttt
     8761 tatattcatt tcttacaaaa tcagttaacc gaacaaaata atccttgtca aattctggct
     8821 gtaaaattgt ttgccactga ggttctattt ttacattcat atctttaaaa attaaaatga
     8881 ttcaaatgca ctttttaatt gtttcattgc ttgctgcaac acatttcgtg gacaagcaac
     8941 attcaacctg aaatgttgct cgccacccgg tccaaaaatt ttccccttat taagtcctaa
     9001 accagctttt tggataaaaa attgaaataa ttcttcatcg gtcattccca ttttttcaca
     9061 attcagccag attaagaaag aagcttctgg tttcataggc tcaattagtg gaagctcttc
     9121 ctttaaaaaa tgaataacat attccacatt tttattgaca tattgcaaca tttgccgtcg
     9181 ccattctgct ccttgtgtgt aagcagcaat ggtcgcaatg taagcaaaaa tgttcccatc
     9241 ggttattctt aactgattca gaaaatcttt gaataaattt ctaatttctt catttggaat
     9301 aatataataa gctgcttgca atgaaggtat gttaaaaatt ttgcttggcg acataagcgt
     9361 aatgctgatt tgtgcagctt tattggacac gctggcaaaa ggaacatgtt gatttcttgc
     9421 caaagccata tcgccatgaa tttcatcaga aacaatcaaa atatgatgac gctcgcaaat
     9481 ttcagccaac gtttgcaagg ttgttcgatc ccaaattcta ccaccaggat tttgaggatt
     9541 acacaacaaa agcaacttgg ttcgagaaga aattttgctt tctaaatctt caaaatccat
     9601 ttcaaatttt ccgtttcttt cctgcaaagg attataaact aatttacgat gattactttt
     9661 tactacttcc ataaatgggt agtaaacagg cggttgaaca atcacttcat ctctttcgct
     9721 ggaaaattgt ttcactgcta aggctaaacc tgttactact cctggtaaaa aacccaacca
     9781 ttcttctttt acctcaaatt gatgaatttg atttaaccaa taaataatac ttgggaaata
     9841 ttcttttggc attatcgtat aacccaaaat gggatgttta caacgacgac gaatggtttc
     9901 taaaataaaa tctggtgtgc gaaaatccat gtctgctacc cacaaaggaa gaacatcgtt
     9961 tgttccaaaa agagaaggac aagcttctat tttcaccgca ttggtatttc tacgatcaat
    10021 aatttcatca aaattatatt tcataaattt ttacttatct tttctctttt cagaataaat
    10081 cttttatttt atttctcgaa ttatttcttc tattggtgtt ccaacgcaag cattaggttc
    10141 aggaatattt aaaattttgc ttaatgtagg agnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nngttccaac gcaagcatta ggttcaggaa tatttaaaat tttgcttaat
    10321 gtaggagcaa tagaggtaac aggataaaaa gtgttgattt tctgagactt aatattccag
    10381 ccataaaaat aaacaggtgt ataagaaatg acagcattcg attcgcccac aggctgatta
    10441 ttttcattta attctatcca gccaggcaat aaagtgagaa tgacatcacc actggtattt
    10501 ttgtgaaatg aatttctgat aggaattatt tgatcattgg tttgcgaatt cgcatttaaa
    10561 acctgagtag ctgtaaaagc agcttgaaca ccttcaaatt ccaacataaa atcagccact
    10621 gtttgttgaa tatcataaag attcaatttt ttttcttcaa ttttttgacg atttaaaaat
    10681 atatttttcc cgtaatagcc ttccacccat ttttcttgac cataaattgc cattaaataa
    10741 gtgtttaaca aagccattga acgatctcca ttaaaatatc ccgctggaat tttattgttg
    10801 cctaattcaa tgggtgtatg aaaattggtc tgattcccaa acatataaac caacacctgc
    10861 gacattccga ttttactatc aattttttgc aacaaatact gaatatcttt atctaattgt
    10921 aaataaatat cttctttttc agcagaattg ataaccgaag ttttttcata aggtgttttg
    10981 acagtaaatt gaagtagaag aaaatcagga taatcgtctg ttcctaattt ttcctgatta
    11041 ataattctat aggcaaggtc agccacaacg gcatttgcag ttggcgtatt tttcaatagc
    11101 aaaagagaag aatttttccg tttactgtca ggtttatatt gaaaagaagt ttttttgttt
    11161 tctccttgtg gtggataaag ataagtatta ggtgaataca tcggttccca aatcatagaa
    11221 gcgtattttc taatctggtc attcacattc atttcatcag cacttgctgc caaaccatct
    11281 gaataataag ttgtggtaat ccatttcatt gtttcgtcac ttatccacac agcactattt
    11341 gccgtatgtc ctgccagcat aagagcttct tcaggattaa tagccactgc gtacgttttt
    11401 gattgatgag aaaaatttag catcaattcg tctgttactg tgctggacaa taaatgatga
    11461 gccgaaacag ttaattttgt tccaatgcca agttcatttt catcgtaaat aatagattgt
    11521 acttccttgt tttttcttga aaaataatga tttcctgtca cgccatgata ataaggagta
    11581 gtgcccgtca taactgtcgc aatgtctgac gcatttcctg ctgaaagaag gttgtaataa
    11641 atattgttac atattgcacc tttttccaca atttgcttga aaccataact atcaaatcga
    11701 ttccacaact ggtcaatatg acgttgttgc aaaccatcaa tcattatccc aacaaccaat
    11761 cgcggtggtt ttgaagtaaa attttgagca gctccataag ttgctaaaac attgaaaatt
    11821 aacaataacc aaaaattccg aacgaaaaac ttcatatttt taaaaattaa ctttttgatt
    11881 tttaataaat taaagacata cgtttataaa gcatcttttg ttgagggtaa atcaaaatga
    11941 taaatatccc gcttgactgc cattttaata gcttttgcca atgctttgaa aatagactct
    12001 aatttatgat gttcattttc accttcagtt tttatgtaaa gattcatttt tgctgcatcg
    12061 ctaaacgatt tgaaaaaatg taaaatcatc tctgttggga agtcaccaat atattctcgc
    12121 tgaaaatggg catcaaaaac caaccatggt cttcctccaa aatcaatagc aaccatactt
    12181 aatgaatcgt ccattggtag gcagaaacca taacgttcaa tgcctcgctt atcagacaat
    12241 gtttttgcaa aagcttcacc aagtgcaaga gccgtatctt caatagtatg atgttcgtcc
    12301 acttccaaat caccttttac ttgaattgtt aaatcacaac ctgaatgttt gctgatttgt
    12361 tccagcaggt ggtcaaaaaa atgaagacct gtagaaatag aacttttacc ttcgccatcc
    12421 aagtttaagt aaacatatac atccgtttcc ttggtggttc gttgtacaac aatcgtccgt
    12481 tcgcctgcaa ataaaaattc agcaattttg tcccaactgc tggtctgcaa aacacaatat
    12541 tctgtcagct tttttttctc taaaattttt tcatcattat ttaagaaaat agctttacaa
    12601 cccagatttt tagcaagttc cacatcagta taacgatcac ctataacaaa agaattttgc
    12661 aaatcataat cttctgaaaa atattcggtc agcattccaa tacctggttt gcgagtagga
    12721 agagattctt caggtaaaga cttatcaata aatatcttat caaaagtaat atcttcgttt
    12781 ttcagaattt gtaaaaattt ctgctgaaca acatcaaaat ttttctgagg ataggctaag
    12841 gttcccaaac catcttgatt agaaaccagt gcccactcaa aatcaagttt ctgacgaata
    12901 aaatataaat tacgaatcac gccaggcaaa aattcaagtt gttccaacgt atctacctga
    12961 taagtttcag atggctctac taaaatggtt ccatctctgt caatgaaaag gactctcttt
    13021 ttcataaaaa atcaaacaat tattaaacaa tcagaattat tttttttata attattctca
    13081 ttacaaaaat aatctatttt tatcatgtag ctaatatata tttcaatttt taaagagttt
    13141 aaaaattgtg taattttgca attgtggaaa tgtttccctt ttaaaacttt aagtgagtat
    13201 gtatgataga ctggatcaaa tcgattttgg tggataatgg tgtagcagaa attatcctgg
    13261 tatattccct tgtcattacc gcaggaattt tactgggaaa aatcaaattt ttcggtgtct
    13321 ctcttggtgt aacttttgtt ttatttgttg gattatttgc tggtcatttg ggcttcaaag
    13381 gaaatccaga agtaatagaa ttcataaaag aatttggact gattttgttc gtgtattcta
    13441 ttggattaca ggttggtcct agtttctttt cttcttttaa aaaaggaggc attcagttaa
    13501 atttattagc taccagtat
//
LOCUS       DAOSAS010000033        13426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3118_length_13426_cov_13.366465, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000033 DAOSAS010000000
VERSION     DAOSAS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13426
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3118_length_13426_cov_13.366465"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..229)
                     /locus_tag="PLR72_02645"
     CDS             complement(80..229)
                     /locus_tag="PLR72_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36142.1"
                     /translation="MTQQVWKQKDIALAIGKDKSVVSRELKRNCDNRNREYRADLAQR
                     KYENK"
     gene            905..2449
                     /locus_tag="PLR72_02650"
     CDS             905..2449
                     /locus_tag="PLR72_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK36143.1"
                     /translation="MKKRTIWILIISMVVTFVGLILLQIRYVKINAEMIENQFDENVQ
                     RSLFQTVRLVEENEALQYLGQILDEPDYKTKENNKKLNFTNEQLQLKDKETNLTQNSK
                     LAQESKELAKIQLSTRQGKSTIEETSHELQKKFFEKFSRSKTILDQAVFRWLKEVENK
                     DISERVNFDELNEILENILNNNGVNLPFNYSIVDKQGRTIYKKTNVTTKNEEVNPKNV
                     YSQKLFPFEETSHPYYLQVIFPTKQSYILHSMNMLLPSIVLVILILVIFIVTIIIIFR
                     QKQLNTMKNDFVNTMTHELKTPIATISLASQMLQDPNIVKTPETLQHLAGLIRDETKR
                     LSFQVEKVLQMALFEKEKAMLNLSEIHINSLIQDVIKSFSLRVTSKGGEIISKLNATD
                     DLALIDEVHFTNVIFNLMDNAQKYTGDKPLILTIETWNEKDNICISIEDNGIGIRKED
                     LKRIFDMFYRVYTGNLHDVKGFGLGLAYVKKIVTDHNGTIKVESEPNIGTKFTITLPT
                     LKKQKQ"
     gene            2446..3156
                     /locus_tag="PLR72_02655"
     CDS             2446..3156
                     /locus_tag="PLR72_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOK36144.1"
                     /translation="MTEEKTKILLCEDDENLGMILREYLQSKGYDVDLMLDGEAGYKA
                     FTHNKYDLCILDIMMPKKDGFTLAADIRAINSNVPIIFLTAKSMKEDILQGFKLGADD
                     FITKPFSMEELLYRIESIMRRIRGEKAREVTVYQIGNYTFDTQKQLLTINGETKKLTT
                     KESELLTLLAANMNNVLERNYALKTIWVDDNYFNARSMDVYITKLRKLLKDDPNVAII
                     NIHGKGYKLIAPQPKDKN"
     gene            complement(3301..7206)
                     /locus_tag="PLR72_02660"
     CDS             complement(3301..7206)
                     /locus_tag="PLR72_02660"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOK36145.1"
                     /translation="MKKVIKYILRILGWIMASIIILLVLAGLLIQTPFVKTKVIDFAE
                     KQVNKSLFAKLEVQTLKGNFFTHLQLKDISLISKNDTILSASSLNVKYQLLPLLHRKI
                     VVNEVSLEQPHIFLVQFADSSWNFQKIIKPQPPKKEKGKPFEMLIDIHHFLLDEASIN
                     IQAFNKQLPQQIAHFNIELTGSYQQNHLAVDLKKLNLKALKPDLQIKNLSFQFAMANK
                     IYKLKNFTLQTAKNNLYADGEINLDFSKNSFFHLKTTHLNFNEFEAFLPKDFQLKVKP
                     DLQLSAQLTQEELQLLLLVKEQQQKIDLQVVSPSFASALRSTKKMEPAYLIHLNFQQI
                     DLRQWLTNSSLNIKLNGKIQAQGSGLNPHSMQTNLKGELTQSVINNNSRIENLNFNFN
                     LQKGNLKGTLTGNSNFGMLSLTTSVDSVLGEYPQYQLDLLTQHLNLAMLTGQNNLNSD
                     LSLQLLLNGAGLKPEQINANTEIHFSPSTIYGLNLQSFFSKIYFQHQNIIIDTLSAAT
                     QTAHLNLNGNYHLKKASNLNLQCSLDSASELKKFVDLPSLETAVQLQAHLEGMPKNWI
                     AQINVSLFNTQFQSYRLKNLTVSANGKIHSAKNYDVKSKIEAQGLNLNNMPLDEINVT
                     AELKPQDIGLEAQINGKDFSTSFHSVVQMEQAIRIGLSDWILKYKDLQWGQDADTAFV
                     KLDKNEYEINHLKLFAYGNDTLPSLSINGKIQTKGTENLNVSLNNIDCQKMITLFSPG
                     LAINGLLNVNVDLKGNATSPILNTKMNIDNFSYQNYKFNNFEATADYEKKILTANVKM
                     IPQDSGQLLIKGKLPIQFRLDTMQFSLLSKKTDPVNLQLSIEKFPLAVLHMFLPKAEI
                     DGFIESNVAVNGTVSEPNLEGQLNITDGKLLWKQYGINYPIILTNLEFQNNNIKLDTF
                     LIKSQKGSMLATGNVQLIPPLNNLKINDAGMKVEFNNFEPISHKYYNMELSGNASLAT
                     NQDSSYFSGEVTIPRSEFYLPAVMRLIGQTSVPEMPKPLLVEEIEKMQPGDSIIIKLR
                     PDTSKQMSKKSSVMLPNLQGNIRVKIPHNTWIKDDNMRLELSGDVEFMKHYNFFELFG
                     SVEVVRGQYNLLGKVFVVESGTVTFAGGEKINPILDLKASYTFHDQARAQRKLVIQVT
                     GETSLPEIAFTLDDEPISEGDAVSYILFGTQLDAISFDQQASINEGLSSSGLAKTAIG
                     SFVSSEITKLLGSAFNVDYVEFKSKGSLSNGSFVVGKYLTNNLFLSYERNFGNFIKEE
                     NVSEYEVKVEYELFHFLFLQLTSSPLRNGVDIVFKINSK"
     gene            complement(7212..9071)
                     /locus_tag="PLR72_02665"
     CDS             complement(7212..9071)
                     /locus_tag="PLR72_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOK36146.1"
                     /translation="MSDFFKKNIFTRYNCSSGLVFFLFFVYCVEVSAQNGYEIGKIKF
                     NGNKSFSKSELLKQTTLHETKWLDKVLSKKKPVIYNENFIQSDIDRLVRFYQCEGFLN
                     ASVELDSVAQNDKKEKVDLYFKIVENKPVLVGEVKIKIEDSLKVKKDSFFLPFRKRML
                     TVSGKRFSDQKIYNDINLIKDFFQNKGFAYAECDQQIHLQPDLSKADVIFSVKLNEKC
                     FFGNTTIEGNKYVKDQLIYRQCTFDSGQVFNKKKIDKTRKHLFDLQLFRIISLQPLLD
                     NTTKRNPIPVKINVEETSRLSTEFGIGYGTEDEFRAFVDVSYRSLFGDASRLHLYLKH
                     SSLEPYHASLSWIQPQFFSKNFSFSVYPFLLRQKEPGYNIQSYGVNLPLTYSISEKMN
                     TSFGYYMENVKQYEGTTNGDYINPESKDYLYDKSGLMFSYNFNNSYPLTSPVRGNVFN
                     ASVKMNGYIFHSDFNYVRCSFDARRYDQIDNFSLAGRILIGGILSSDSSKFVPVEDRF
                     YSGGSNSIRGWGRSRLGPLRDDGSPLGGKSVLEMSVEVRHRLFWLLEGALFFDAGNVW
                     SSSFHYPFNQLAYTAGGGLRINSPIGVVRLDVGFPLWNAKKSPQVFLNIGQAF"
     gene            complement(9566..10906)
                     /gene="sufD"
                     /locus_tag="PLR72_02670"
     CDS             complement(9566..10906)
                     /gene="sufD"
                     /locus_tag="PLR72_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOK36147.1"
                     /translation="MLMAQQYIDFYKSYQQDIKSHSAILLNAFRDEAFAKFEKLGFPT
                     AKLEDYKYTDMKEPLTVDYGLNINHLPIPVNPYKVFKCDVPGIRSYLYFVVNEVFYPV
                     TSTSNQQFPEGVIIDSLMGASEKYPELVEKYMGKLSREKNDGWIAFNETFAQDGFFMY
                     IPKNVKLKKPVQLVNILHSNVDLMANTRNLIIIEEGGEAQLLVCDHTMDNVKFFSNRL
                     TEIFVGENAHYEHYHLENVHQQTTNVSTLLVNQQTSSKVLANIITLHNGITRNSIEVN
                     IDGEFCETLLTGMVIGDKTQTVDNFTSIVHKKPNSQSNELFKYILDENSKGAFSGKIY
                     VAPHAQKTFAYQNNRNILLSKNARMRSKPQLEIYADDVKCSHGATIGQLDELAKFYMQ
                     SRGISEAEARFLLMFAFTNDVIENIKIEALKGRMRLLIEKRLRGKLAYCEGCMI"
     gene            complement(10963..11730)
                     /gene="sufC"
                     /locus_tag="PLR72_02675"
     CDS             complement(10963..11730)
                     /gene="sufC"
                     /locus_tag="PLR72_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOK36148.1"
                     /translation="MLEIRNLYANINGKEILKGVNLTVKAGEVHAIMGPNGGGKSTLA
                     LAIVGYPGVEVTKGEIIFDGKNLLEISPEDRSHEGIFLSFQNPVEIPGVSMVNFLRTA
                     LNEHRKYKNLEPLSAADFLRLMREKKELVEMDNKLTNRSVNEGFSGGEKKRNEIFQMA
                     MLEPRLAILDETDSGLDIDALRIVANGVNKLKRPDNSIIVITHYQRLLDYIVPDYVHI
                     FYDGRIVKTGDKTLAAELEEKGYDWIKKELEEKELQY"
     gene            complement(11816..13252)
                     /gene="sufB"
                     /locus_tag="PLR72_02680"
     CDS             complement(11816..13252)
                     /gene="sufB"
                     /locus_tag="PLR72_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOK36149.1"
                     /translation="MSSDILDEITQSEYKYGFTTNIDTEIIPPGINEDIIRLISSKKN
                     EPEWLLNFRLKAYRHWLTMKMPTWAHLKIPPIDFQKISYYAAPRKKSPKSMYEIDPEL
                     IKTFEKLGIPLEEQKVLTGMAVDAIMDSISVKTTFQENLSELGIIFCSISEAIKHHPD
                     LVQKYLGSVVPYSDNFFASLNSAVFSDGSFVYIPKGVRCPMELSSYFRINANNTGQFE
                     RTLIIADQDSYVSYLEGCTAPMRDENQLHAAVVEIVVLKNAEVKYSTVQNWYPGDKEG
                     KGGVFNFVTKRGICKEENAKLSWAQVETGSAITWKYPSCILQGDSSSAEFYSVAVTNH
                     YQQADTGTKMIHIGKNTHSHILSKGISAGYGQNSYRGLVQMSKNADNARNYSQCDSLL
                     LGDKCGAHTFPYMDVSNESAIVEHEATTSKISEDQIFYCNQRGISTENAIGLIVNGYA
                     KEVINKLPMEFAVEAQKLLQITLEGSIG"
BASE COUNT         4512 a         2236 c         2079 g         4599 t
ORIGIN      
        1 gactgaaatc ctttgcaagg cattcttcta catattgttt tacttcttct gtaaagcgga
       61 tatgtttagg cttattcttt tacttgtttt cgtatttgcg ttgtgccaaa tctgctctat
      121 attccctgtt tcgattatcg caattacgct ttagttcacg cgatacaacc gatttatctt
      181 tcccgatagc taatgctata tctttttgtt tccatacttg ttgggtcatt gcccaaattt
      241 atacttttat tctacggtta aatgcttctt tgtaactttt tatagaaaaa agacaaagta
      301 aagcatttat tccttcaaga caaaagagaa aaacgttttt ctcttttgtc ttgataaata
      361 attttccttt accttttatt tccctttaaa aagttgcatt aattacttga atttacgcct
      421 aattagtttg ttctttcaaa aaactttttt gctattagga gaagtttaaa aaccaattat
      481 tctggattta tccctaccaa tgaatgaatc taattgaaaa ctttccattc tacttgagac
      541 tatcaattgg cataaaaatt gtaaagttta cttttaaaac atttaatttt ataaattaaa
      601 gtttataaaa gttttttgaa gaggcaaaat taatttttag ttagtttttt tattttcagt
      661 ggaggaagat agaatttttt taaatattat tcttactcaa ttacttgatt ttaaattaca
      721 tttgaaactt atcatactaa aaataagttt cattaaacta aaatactttg aataacgttt
      781 attatataaa aaatatcaat tttcaataat aaaaaatttc atttttgttg tatttaatat
      841 aattatgtta aatattatga aaaattatct taaatattta tatttagtgt aaatttgttt
      901 cattatgaag aaaagaacta tttggatatt gattatttca atggttgtta catttgtagg
      961 tttaattttg ctacaaattc gctatgtgaa gatcaatgct gaaatgattg agaaccagtt
     1021 tgatgaaaat gtacaacgaa gtttattcca aacagtgaga ttggtagaag aaaatgaggc
     1081 attacaatat ttaggacaaa tattagacga accagattat aaaacgaaag aaaataacaa
     1141 aaaattaaat tttactaacg aacagttaca gttaaaagat aaagaaacta atttaacaca
     1201 aaattcaaaa ttagctcagg aatcaaaaga acttgctaaa attcagttgt ctaccagaca
     1261 agggaaatcc actattgaag agacctcgca tgaattgcaa aaaaagtttt ttgaaaaatt
     1321 ttcccgatca aaaactattt tagatcaagc agtttttaga tggttaaaag aagtagaaaa
     1381 taaagatatt tcagaaagag ttaattttga tgagcttaat gaaattctcg aaaacatttt
     1441 aaataataat ggcgtaaatt taccatttaa ttattctatt gttgacaaac aaggcagaac
     1501 aatttataaa aagactaatg taacgactaa aaatgaggaa gttaatccaa aaaatgtata
     1561 ttctcaaaaa ctttttccgt tcgaagaaac cagtcatcct tattatttgc aggtgatttt
     1621 tcccacaaaa caaagttaca ttttgcattc aatgaatatg ttgctacctt caattgtttt
     1681 agtgattttg atactggtaa tttttattgt aactatcatt atcattttca ggcaaaagca
     1741 attaaatact atgaaaaatg attttgtaaa tactatgact cacgaattaa aaacacccat
     1801 tgctaccatt tcattagcat cacaaatgtt gcaagacccc aatattgtaa aaacaccaga
     1861 aacactacaa catttggcag gccttattcg tgatgagacc aaacgattaa gtttccaggt
     1921 agaaaaagtg ttacagatgg cactttttga gaaagaaaaa gcaatgttaa acttgagtga
     1981 aattcatata aactcattga ttcaagatgt tatcaaaagc ttttcattaa gagttaccag
     2041 taagggtggc gaaataattt cgaagttaaa tgctacagac gacttagctc tgatcgatga
     2101 agtccacttt actaatgtta tttttaattt aatggacaat gcccaaaaat acactggaga
     2161 taaaccttta attttaacca ttgaaacatg gaatgaaaag gacaatatct gtatcagtat
     2221 agaagataac ggtatcggaa tccgaaaaga agacctgaaa agaatatttg atatgtttta
     2281 tcgggtttat acaggcaatt tgcatgatgt taaagggttt gggttaggat tagcttatgt
     2341 aaaaaaaatt gtaacggatc acaatggaac tattaaagtg gaaagcgaac caaatatcgg
     2401 aactaaattt actattacat taccaactct aaaaaaacaa aaacaatgac agaagaaaaa
     2461 actaaaattt tattgtgcga agatgatgaa aatcttggca tgatattaag agagtatcta
     2521 caatcaaaag gttatgatgt agatcttatg ttagatggtg aagctggtta taaagctttt
     2581 acgcataata aatacgattt atgcatattg gatataatga tgccaaaaaa agatggcttc
     2641 acactggctg ctgatattcg tgctatcaac agtaatgtac ccatcatttt ccttactgca
     2701 aaatcaatga aggaagatat tcttcaagga ttcaagttag gtgctgatga ttttattaca
     2761 aaaccattca gtatggagga attgctctat cgtattgaat ccattatgcg tagaatcaga
     2821 ggcgaaaaag ccagagaagt aacggtttat caaatcggta actacacttt tgatacccaa
     2881 aaacaactgc ttaccatcaa tggggaaact aaaaaactga ctaccaaaga gtcagaacta
     2941 ctcactttat tggctgccaa catgaataat gttttagaac gcaattatgc tttaaaaact
     3001 atttgggtag acgacaatta ttttaatgca cgcagcatgg atgtttacat taccaagctt
     3061 cgtaaactgt tgaaagacga tccgaatgta gccatcatta acattcatgg gaaaggctac
     3121 aaattaattg caccgcagcc aaaagacaaa aattaaatta tcaaattttt ctttaacaag
     3181 cgagggaatt ccacaaagga tttcctcgct ttgtaataag ggttttttca aaaaactaaa
     3241 acaaaaatat ccaccttaat attatctaaa aaacaagaag aaattattgc atcttttaat
     3301 ctattttgaa tttatcttaa aaacaatatc tacaccattt cgtaaaggag aactggttaa
     3361 ttgtagaaaa agaaaatgaa acagttcata ttcgactttt acctcgtatt cacttacatt
     3421 ttcttctttg atgaaattac cgaaatttcg ttcatagctc aaaaacaaat tattggtaag
     3481 atattttcca actacaaatg agccgttact cagggaacct ttactcttga attctacata
     3541 atctacatta aacgcacttc ccaataattt agtgatttca gaagagacga aagagccaat
     3601 tgctgtttta gccaagcctg agctactcag tccttcgttt atgcttgctt gttgatcaaa
     3661 ggaaatagca tcgagttgtg taccgaacaa aatataggaa acagcatcgc cttcactaat
     3721 aggttcgtca tcaagtgtaa aagcaatttc tggcaaagaa gtttcacccg ttacttgaat
     3781 caccaatttt cgttgtgctc ttgcctgatc atgaaaggta taacttgctt ttaaatccag
     3841 gatgggatta attttttctc cacctgcaaa ggtaacggtc cctgattcca ctacaaaaac
     3901 ttttcctaac aaattatatt gtcctcttac cacttctaca gaaccaaaaa gttcgaagaa
     3961 attgtagtgt ttcataaatt ccacatcacc agacaattcc agacgcatat tgtcatcttt
     4021 tatccaggta ttgtgcggaa tttttactcg tatattccct tgcaaattgg gaagcatcac
     4081 gctacttttt tttgacattt gttttgaagt gtctggtctt aatttaatga ttatggaatc
     4141 gcctggctgc attttttcaa tttcttcaac caacaaaggt ttgggcattt caggaacaga
     4201 agtttgaccg attaatcgca tcacagcagg caaataaaat tctgaacgtg gaatggtaac
     4261 ttcacctgaa aaataagaac tatcctgatt ggtggcaaga gaagcatttc ctgataattc
     4321 catgttgtaa tatttatgac tgatcggctc aaaattgtta aattccactt tcattccagc
     4381 atcatttatt tttaaattat tcagtggcgg aatcaactga acatttccag ttgccagcat
     4441 ggaacctttc tgacttttta tcaagaaagt atccagtttt atgttattat tctgaaattc
     4501 cagattggtt aatattattg gataattaat tccatattgt ttccataata attttccgtc
     4561 tgtaatattg agttgacctt ccagattagg ttctgaaaca gtgccattta ctgctacatt
     4621 actttcaata aaaccatcaa tttcggcttt cggtaaaaac atatgcaata ctgccagcgg
     4681 aaatttttct atagaaagtt gcaagttaac aggatcggtt tttttagaaa gcaagctaaa
     4741 ttgcattgtg tcaagccgaa actgtatagg aagttttcct tttatcagca attgtccact
     4801 atcctgtgga atcattttta catttgcagt caagattttt ttttcataat cagccgttgc
     4861 ttcaaagtta ttgaatttat aattttgata ggaaaaattg tcaatattca ttttggtatt
     4921 taaaatgggc gaagttgcat tgccttttaa atcaacattt acgttcaata gaccatttat
     4981 tgccagtcct ggagaaaaca aagtaatcat tttttgacag tcaatgttgt tcaatgaaac
     5041 atttaaattt tccgtgcctt ttgtttgtat tttgccattt atggaaagac ttggcagggt
     5101 atcatttcca taagcaaaaa gttttaaatg gttgatttca tattcgtttt tatccagttt
     5161 cacgaaagca gtatcagcat cttgtcccca ttgtaaatct ttgtatttca atatccagtc
     5221 tgataaacca atacgaatcg cttgttccat ttgaacaaca gaatgaaaag aggtagaaaa
     5281 atcttttcca tttatttgcg cttccagacc aatatcttgc ggcttgagct ctgcagttac
     5341 attgatttcg tccagaggca tattatttaa atttaagcct tgagcctcaa ttttgctttt
     5401 cacatcataa ttttttgctg aatgaatttt tccgtttgct gaaacagtga gatttttaag
     5461 acgataagac tggaattgag tattgaaaag actgacattt atttgtgcga tccaattttt
     5521 cggcattcct tccagatgag cttgcagttg tacagcagtt tccagcgaag gaagatcaac
     5581 aaatttttta agttcgctgg cactatcaag tgaacattgc aaattcaggt ttgatgcttt
     5641 ttttaaatga taattcccat tcagatttaa atgagctgtt tgcgtcgctg ccgaaagtgt
     5701 gtcaatgata atattttgat gttgaaaata tatttttgaa aaaaatgatt gtaagttaag
     5761 tccatagatg gtagagggtg agaaatgtat ttcagtattc gcattgattt gttctggctt
     5821 taagcctgct ccattcagta ataactgtaa agataaatcg ctgtttaagt tattttgacc
     5881 cgttagcatg gccagattca aatgttgtgt cagtaaatca agttggtact gaggatattc
     5941 acccaaaaca gaatcaacag aagtcgttaa ggaaagcatg ccaaaattac tatttcctgt
     6001 gagcgtacct ttcagatttc ctttttgcaa attaaaattg aaattcaaat tttcaattct
     6061 ggaattattg tttatgacag attgtgtcaa ttcaccttta agattagttt gcatcgaatg
     6121 tgggttcaat ccactacctt gagcctgtat tttaccattc agctttatgt tgagagaaga
     6181 atttgtcaac cattgacgaa gatctatttg ctgaaaattc agatgaatta aataagctgg
     6241 ttccattttt tttgttgagc gaagcgcact ggcaaaagaa ggggaaacca cttgaagatc
     6301 aattttttgt tgttgctctt ttaccaataa aagcaattgc aattcttctt gagtcaattg
     6361 ggcagaaagt tgtaaatctg gctttacttt tagttgaaaa tctttcggta aaaaggcttc
     6421 aaactcattg aaattcaggt gtgtagtttt taaatgaaaa aatgagtttt tagaaaaatc
     6481 aagatttatt tctccatcag cataaagatt gtttttagca gtttgcaatg tgaaattttt
     6541 gagtttataa attttgtttg ccattgcaaa ttgaaaactt aaatttttta tttgcaaatc
     6601 tggttttaaa gctttcaaat tcaatttttt caaatctaca gctaaatggt tttgctggta
     6661 gctacctgtt aattcgatat tgaaatgagc aatttgttgc ggcaattgtt tattaaacgc
     6721 ctggatatta attgaggctt catcaagcaa aaaatgatga atatcaatca acatttcaaa
     6781 aggttttcct ttttcttttt tgggtggttg aggctttatt attttttgaa aattccagct
     6841 tgaatccgca aactgaacaa gaaaaatatg aggttgttcc aaactgactt cattgacaac
     6901 aatttttctg tgcagcaaag gcagtaattg atattttaca ttcaatgacg aagcggaaag
     6961 aatcgtgtca ttttttgaaa taagagaaat atcctttaac tgtaaatgtg tgaaaaaatt
     7021 tcctttcaaa gtttgtactt ccaattttgc aaaaagagat ttatttactt gtttctcagc
     7081 aaaatcaatc acttttgttt ttacaaaagg tgtttgtatc aacaatccag caagcaccaa
     7141 taaaatgatg atacttgcca ttatccagcc taaaattcgc aaaatatatt ttattacttt
     7201 tttcattttt gctaaaaagc ttgaccaata ttgagaaata cttgtggtga tttttttgca
     7261 ttccataatg gaaatcctac gtccagtctt actacaccaa ttggcgaatt aatccgtaaa
     7321 ccaccgccag ccgtataagc caattgattg aatggatagt gaaaagaaga agaccataca
     7381 tttcctgcat caaaaaacaa agctccttcc agcagccaga ataaccgatg tcgaacctct
     7441 acactcattt ccaaaacact tttcccaccc agaggtgaac catcatcacg caaaggaccc
     7501 aaacgagacc ttccccaacc tcgaatagaa ttactgccac cagaatagaa acgatcttct
     7561 actggaacaa acttactgct atctgaagac aaaattccgc caatcaagat acgtccagcc
     7621 aatgaaaagt tatcaatctg gtcatatctt cttgcatcaa aactgcaacg tacataatta
     7681 aaatcactat gaaaaatgta accgttcatt tttacactgg cattgaacac atttccacgg
     7741 acaggagaag tcagtggata agaattatta aaattgtaag aaaacatcaa gccggattta
     7801 tcatacaaat aatctttgct ttcaggatta atataatcac catttgtagt gccttcatat
     7861 tgtttcacat tttccatata ataaccaaaa gaagtattca ttttttcgga aatagagtag
     7921 gtgagaggca aatttacacc ataactttga atgttatagc ctggttcttt ctggcgaagc
     7981 aaaaaaggat aaactgaaaa tgaaaaattt tttgaaaaaa attgtggctg tatccagctc
     8041 aaactggcat gataaggttc taacgacgaa tgttttaaat acagatgaag acgtgaggca
     8101 tcgccaaata aggaacgata actgacatca acaaaagctc gaaattcatc ttctgtgcca
     8161 taaccgatgc caaattctgt acttaaacgg gaagtctctt cgacattaat tttcacagga
     8221 ataggatttc ttttggttgt attatccagc agaggttgga gagaaataat tctaaaaagc
     8281 tgtaaatcaa ataaatgttt tcgtgtttta tcaattttct ttttgttaaa aacctgtccg
     8341 gaatcaaaag tacattggcg ataaatcaat tggtctttta catatttatt accttcaatg
     8401 gttgtatttc caaaaaaaca tttttcattt aatttaacgg aaaaaatcac atctgcctta
     8461 ctcaaatctg gttgtaaatg aatttgttgg tcacattctg cataagcaaa acctttattt
     8521 tgaaaaaaat ctttaataag attaatatca ttatagattt tctgatctga aaaacgtttt
     8581 cctgaaacag tgagcattct ttttctgaaa ggtaaaaaga aagaatcttt ttttactttt
     8641 aaactatctt caatttttat ctttacttca ccaaccagca cgggcttgtt ttccacaatt
     8701 ttaaaatata aatccacttt ttccttttta tcgttttgag ccacggagtc aagttccacg
     8761 ctggcattta aaaaaccttc gcattgataa aaacgaacta accgatcaat gtctgattgg
     8821 ataaaatttt cattataaat aaccggtttt tttttggaaa gtactttgtc taaccatttt
     8881 gtttcgtgca aggttgtttg ttttaatagt tcagatttgc tgaaagactt gttaccgtta
     8941 aatttgattt taccaatttc ataaccattt tgagctgaca cctcaacaca atagacaaag
     9001 aaaaggaaaa atacaagtcc ggaagaacag ttatatcgcg taaaaatatt ttttttaaaa
     9061 aaatcactca ttaacttttg taattgaaat ttttaaaaaa tttgaacata tcacaaaaaa
     9121 tacgtaaccc aaagaatgta aggtttactt tttgctactg acaaaattag aatttaattt
     9181 caaattttaa gccaaaatta tcaaaatgaa ttatttgaat ttacgattta atatcaaatt
     9241 gtattcaaaa aggagaaata aatcgaaaat agtagggaat caataggttt gatgtgatgg
     9301 atagggaata catgaggcaa gaaaacgagg atttttatag agaatattgt tttacgtctt
     9361 gataacttat cgtaagagaa tatgatttat gccaagacaa ttgaagcgaa gcgaaatcac
     9421 cagaatgttt aaaattaaat atttcttgta acaaaaagaa aacgcagtct tgcacaaagc
     9481 ttcctattga aaaacaacat tttgtatatt ccttgatgtc aacgtcaagt tatttcaaat
     9541 caaattttat ttttacaact cagtattaaa tcatgcagcc ttcgcaatag gctaattttc
     9601 ctctcaaacg cttttcaatg agcaatcgca tccttccttt cagtgcttcg attttgatgt
     9661 tttctatgac atcattagta aaagcaaaca tcaacaggaa acgagcttca gcttcagaaa
     9721 tgccacgact ttgcatgtaa aattttgcca attcatccaa ttgaccaata gttgctccgt
     9781 gagagcattt tacatcatct gcataaattt ccaattgagg cttggatcgc attctggcat
     9841 ttttgctcag caaaatattt ctgttgtttt gataagcaaa agttttttga gcatgtggag
     9901 ctacatatat ttttccacta aaggcacctt ttgaattttc atcaagaata tatttgaata
     9961 attcgttgct ttgcgagtta ggttttttat gaacaataga agtaaaatta tcaactgtct
    10021 gagttttgtc gcctataacc attcctgtaa gtaaagtttc gcaaaattca ccatctatgt
    10081 ttacctcaat ggaattccgt gtgataccat tatgcaaagt gataatatta gcgagcactt
    10141 ttgaagaagt ctgctgatta accaataaag tagaaacatt ggtagtttgt tgatgtacat
    10201 tttccagatg ataatgctca taatgggcat tttcaccaac aaaaatttcc gtcaaacgat
    10261 ttgagaaaaa tttcacgtta tccattgtgt gatcgcaaac cagcaattga gcttctccac
    10321 cctcttcaat gataattaaa ttgcgtgtat ttgccattaa atcgacattg gaatgtagaa
    10381 tatttactag ttgcactggt tttttcagtt taacattttt tggaatgtac ataaaaaaac
    10441 catcctgagc aaacgtttca ttaaatgcaa tccagccgtc atttttttct ctgcttaatt
    10501 tacccatata tttttctacc agttcagggt atttctctga agctcccatt aaactgtcaa
    10561 tgatcacacc ttctggaaat tgctgattcg aagtcgaagt cacaggataa aaaacttcat
    10621 tgactacaaa atataaataa gaacgtatgc caggaacatc gcacttaaat actttataag
    10681 gatttacagg aattggcaag tgattgatat ttaagccata atccactgtc aaaggttctt
    10741 tcatatcagt gtatttgtaa tcttcaagct tagcggtagg aaagcctaat ttttcaaatt
    10801 tagcaaaagc ttcatctcga aaagcattca acaaaattgc tgaatgtgat tttatatctt
    10861 gctgataact tttataaaag tctatatatt gttgtgccat aaggatttgt aatattcaaa
    10921 acatttttcg ttgtcaaatt aaaaatctga tatttttcac gtttaatatt gaagttcttt
    10981 ttcttccaat tcttttttaa tccagtcgta tcctttttct tcaagttcgg ctgccagtgt
    11041 tttatctcct gttttcacaa tgcgtccgtc ataaaaaata tgaacataat caggaacaat
    11101 ataatccaat aaacgttgat aatgagtgat tacaataata gaattatcag gacgtttcaa
    11161 tttattcact ccatttgcca caatgcgtaa ggcgtcaatg tctaagcccg aatctgtttc
    11221 gtccagaatt gccagtcgtg gttcgagcat agccatctga aaaatttcat tccgcttctt
    11281 ttcgcctcct gaaaaacctt cattaacaga gcgatttgtt aatttattgt ccatttccac
    11341 caattctttt ttttcacgca tcaaacgcag aaaatcggca gccgaaagag gttctaaatt
    11401 tttatatttc cgatgttcat ttaaagcagt gcgtaaaaaa ttaaccatac ttacgccagg
    11461 aatttctaca ggattctgaa aacttaagaa aattccttcg tgggaacgat cttcgggcga
    11521 aatttccagt aggtttttcc catcaaaaat aatttcacct ttcgttacct ctactccagg
    11581 ataacctaca atagctaatg caagggttga ttttccacct ccatttggtc ccataatggc
    11641 atgaacttca cctgctttta cggttaaatt tacacctttt agtatttctt tcccattaat
    11701 atttgcatac aaatttctaa tttcaagcat atcatttatt ttttcactca aacaacaata
    11761 aattgatttt caataaattt aaaaaataat tttcttcttt tcaaccattc atatattatc
    11821 caattgaacc ttcgagtgtt atttgcaata atttttgtgc ttcaacggca aattccatcg
    11881 gtaatttatt aataacttct ttggcataac cgtttacaat tagcccaatg gcattttcgg
    11941 tagaaatacc ccgctgattg caataaaaaa tctgatcttc actgattttt gaagttgttg
    12001 cttcatgctc tacaatagca ctttcgttgc tcacatccat ataggggaaa gtatgagcac
    12061 cacatttgtc acccagcaat aaactgtcgc actgagaata atttcgtgca ttgtctgcat
    12121 tttttgacat ttgcaccagt cctctgtagc tattttgacc ataaccagcc gaaattcctt
    12181 ttgaaagaat atgactgtga gtgtttttac ctatatgaat catttttgtg cctgtgtcag
    12241 cttgctgata atgattggtt acagcaacag aataaaattc agcactggaa gaatcccctt
    12301 gcaaaataca actggggtat ttccatgtga tagcagagcc tgtttcaact tgtgcccacg
    12361 aaagttttgc attttcttct ttgcaaatac ctcgttttgt tacaaaatta aaaaccccac
    12421 cttttccttc tttatcacca ggataccagt tttgtacggt agagtatttt acttcagcat
    12481 tttttaaaac cacaatttcc acaacggctg catgcaattg attttcatcc cgcatgggag
    12541 cggtacatcc ttccagataa gaaacataac tatcttgatc ggcaataatc aaggttcttt
    12601 caaattgacc ggtattattg gcattgattc gaaaataact cgaaagctcc attggacaac
    12661 gtactccttt agggatataa acaaatgaac catcactaaa aactgcactg tttaaagagg
    12721 caaagaaatt atcactatac ggaaccactg atcccaaata tttctgaacc aaatcaggat
    12781 gatgttttat tgcttcacta atagaacaaa aaatgatgcc taattctgac agattttctt
    12841 gaaaggtagt ttttacagaa atgctatcca tgatggcatc tactgccatg ccggttaaga
    12901 ctttttgctc ttcaagtgga attcctaatt tttcaaaagt tttaataagt tctggatcaa
    12961 tttcatacat gcttttcgga cttttttttc gtggagctgc ataataagat attttttgaa
    13021 aatcaatggg tggaattttt aaatgagccc aggtaggcat tttcatagta agccaatgac
    13081 gataagcttt caatctaaaa ttgagtaacc attcaggctc gtttttttta gaagaaatca
    13141 gtctaataat atcttcattt atgccaggag gaatgatttc ggtatctata ttggtagtga
    13201 aaccatattt atattcactt tgggtaattt catctaatat atctgaagac ataaaaaaat
    13261 ggtttgaaga aaaattttca agtaaataaa gaatatttca aattattgtt acaataacag
    13321 ttgattattt gattttgttt aaaaaagcgt tttctttagt tttttaaaaa atttattttt
    13381 caagaacgtt agtttaaaac tatgcacttt tagtgcaaga ctttag
//
LOCUS       DAOSAS010000034        13187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3190_length_13187_cov_14.417682, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000034 DAOSAS010000000
VERSION     DAOSAS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13187
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3190_length_13187_cov_14.417682"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLR72_02685"
     CDS             <1..380
                     /locus_tag="PLR72_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK36150.1"
                     /translation="KKYSNGTEPEEYQFEVVWSIEHDLIIEEEANQKKTSLLKALDKL
                     TPRQKEVIYLRFTKELDYCSIAEIMNISIESCRNLISKAIFNIKKGKREKIKFAINIF
                     YLSPNGRNISEVVFDAILLSVIM"
     gene            complement(453..526)
                     /locus_tag="PLR72_02690"
     tRNA            complement(453..526)
                     /locus_tag="PLR72_02690"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(490..492),aa:Met,seq:cat)
     gene            complement(706..2169)
                     /locus_tag="PLR72_02695"
     CDS             complement(706..2169)
                     /locus_tag="PLR72_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOK36151.1"
                     /translation="MVDLQEWNNESIKEYLQHPIFKIISETADEQQEECYIIGGFVRD
                     IFLKRSSKDIDIVVIGSGIELAKRVVEKLDKKAKLTVFKNFGTAQVKTNDLEIEFVGA
                     RKESYRSDSRKPIVENGTLEDDQKRRDFTINTLALCLNKNRFGELVDPFNGYRDLQNS
                     VLRTPLDPDITFSDDPLRMLRCIRFATELNFYVEVNTFEAIRRNKKRIEIISKERITD
                     ELNKIILSKKPSIGFILLDQSGLLELIFPELFALKGVETKDGIGHKDIFYHTLAVLDN
                     LSFQTDSLWLRWAALLHDIGKPLTKCFDKQTGWTFYNHNVVGSKMVADIFRKMRLPVN
                     EKMKYVQKLVFLHMRPIVLSEEEVTDSAVRRLLFEAGNDIDDLMMLCEADITSKNPEK
                     VKQYRNNFQLVRKKLKEIEEKDRIRNFQPPIDGNEIMKTFNLPACATVGEIKQRIKDA
                     ILDGVIPNEYEAAREYMMKIGKEMGLMPSLINNQEKK"
     gene            complement(2460..5318)
                     /gene="polA"
                     /locus_tag="PLR72_02700"
     CDS             complement(2460..5318)
                     /gene="polA"
                     /locus_tag="PLR72_02700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOK36152.1"
                     /translation="MEKKKLFLVDAHALIYRSYYAFIRNPRINSKGQNTSAIFGFVNT
                     LEEVLKKEQPTHMAVAFDPEGKTFRHEAFEQYKAQREMTPEDIRKAIPIVKELVDAYN
                     IPILEVPGYEADDVIGTIAKQAEKAGYDVYLLTGDKDFGQLVSDHIFMYRPHHNGGFE
                     ILGPEEIKAKYQLNSQQQVIDLLGLMGDSSDNIPGCPGIGEKTAIKLLQEFGSIDNLL
                     KNTDKLKGALRQKIEENKDLILFSRFLATIKTDVPVSFDEEKFKISPVNETKLRALFK
                     ELEFRTLLANKFGNSTILFPTDVSAKNVDETKKNKEGQQMFLFGTETPQTLSETKEKS
                     ENEKITVGEEISPDGYLTLRNVPHRYQLVDTKAKRAYLISQLFTQKSVCFDTETTSLD
                     VFNADLVGFSFCFKEGEAYFVTLPTDRKEAAIILKEFKAFFQSEQIEKIGQNLKFDYL
                     MLSMYNIELRGKLFDTMIAHYLLQPELRHSMDYLAEIYLHYRTIHYEDLVGPKGKNQA
                     DIRNIEIAKLCEYACEDADITFRLKQILEKEILENGLEKLFYDIEMPLMRVLAIMEQT
                     GVRIDTEVLQQSSKILTNEMLKIEKEIYQMVGFEFNVSSPSQVGEVLFEHLRLDDNAK
                     KTKTGQYSTSEDILEKLRNKHPVVGKILEYRNIKKLLSTYIDALPKLINPQTGKIHTT
                     YNQINTATGRLSSSNPNLQNIPIRDDIGKEIRRAFIPEENSLFFSADYSQIELRIMAH
                     LSNDEAMVEAFKKGQDIHAATASKIYKVPIEEVTPEMRRNAKTANFGIIYGISAFGLA
                     ERLGISRTEAKNFIDEYFNTYPGVKSYIDNIIQKAREKGYVETLLGRKRFLPDINSQN
                     SVVRGFAERNAINAPIQGSAADIIKIAMVNIQHRLEQENLQTKMIIQVHDELDFTVPK
                     EEVETVRELVIYEMEHAFQLNVPLIVDCGIGNNWLEAH"
     gene            5496..6470
                     /locus_tag="PLR72_02705"
     CDS             5496..6470
                     /locus_tag="PLR72_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOK36153.1"
                     /translation="MNVIEIIKKPIIKELHSFEKQLVNELKTENPLLTNINEYIQQGK
                     GKQLRPILMFLSAKICGAINEQTIISAISIELLHTASLIHDDVVDNAFERRGRPSVNA
                     RWDNKIAILAGDYLLSKSLNCAIKANNLEILHVIAKIGVELTDGELLQLENMQKIKIT
                     EDDYYKIIRKKTALLFSSCCIIGGLSVNANQTLLKKLKNFGEYVGICFQIKDDIFDYS
                     DSKNIGKPRRNDIREGKITLPLIYALQQADEKKKEKIINWIKKNKFSEKNIEKITNFV
                     YKNGGIEYATSKLIEFKNKALTELDSFEESEIKQSLKYFAEYMVTREE"
     gene            complement(6471..7355)
                     /gene="deoC"
                     /locus_tag="PLR72_02710"
     CDS             complement(6471..7355)
                     /gene="deoC"
                     /locus_tag="PLR72_02710"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOK36154.1"
                     /translation="MKDKYQELFDFYHCEVNDEAVKIEIDKILAEHFAENANKEVYNQ
                     CLNLIDLTSLNCTDTESEIATLTEKVNNFKNVFPHLSNVAAICVYPALVPVVKKHLND
                     QKVKIASVAGGFPASQTFLEVKIAEASMAVQAGANEIDMVISVGKFLEEKYEEVYDEL
                     SEIKATCRDAHLKVILETGALPNCTNIKKAALLAMASGADFVKTSTGKIQVSATLEAT
                     YTICHAIKEWYGKTGSKIGYKPAGGISTTEEAVKHFTLVKHILGNEWLNNQWFRFGAS
                     RLANQLLSSIEGKEIKYF"
     gene            complement(7377..7703)
                     /locus_tag="PLR72_02715"
     CDS             complement(7377..7703)
                     /locus_tag="PLR72_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOK36155.1"
                     /translation="MTIKEAQQQVDNWIKKYGVRYFSELTNMVLLTEEVGELARIIAR
                     TYGEQSSKAEEEKTNLSDEMADVLWVLICLANQTGIDLTEAFEKNIKKKTERDAQRHQ
                     QNKKLH"
     gene            complement(7672..8376)
                     /locus_tag="PLR72_02720"
     CDS             complement(7672..8376)
                     /locus_tag="PLR72_02720"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOK36156.1"
                     /translation="MKRINIHKEGRFILSGLLLLIFCINLVIYLKFPVTVVAINTILS
                     ALVLCFFTFFFRSPKRIFEIDDPSLVVAPADGTVVVIEPTLETEYFGDERIQVSIFMS
                     IFNVHANWYPVSGKVIKTSHRGGRHLAAYLPKSSKENEQASIVIETPLKTQILVRQVA
                     GALAQRIVTYAYPGKECHINEHLGFIKFGSRVDLFLPLDTEIFVKVGEKTTGNETIIA
                     RLKENKDDNQGSSTAS"
     gene            8604..9947
                     /gene="purB"
                     /locus_tag="PLR72_02725"
     CDS             8604..9947
                     /gene="purB"
                     /locus_tag="PLR72_02725"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOK36157.1"
                     /translation="MNLSPLTAISPIDGRYHSKTKMYASYFSEFALIRCRVQIEIEYF
                     IALCEIPLEQLKQISADIFPMLRNIYQNFQETDAQKIKDIEQITNHDVKAVEYFLKKK
                     FEEIGLSKYKEFIHFGLTSQDINNTAFPFLLKAALEDVYYAELKKLLEELDQLAENWK
                     DIPMLARTHGQPATPTRLGKEIMVFSYRIKEQLELLKQIPISAKFGGATGNFNAHAVS
                     YPAIDWKKFADNFLSDKFGLKREQYTTQISNYDHLSALLDATKRINTVLIDLCRDMWT
                     YISLEYFKQKIKANEVGSSTMPHKVNPIDFENAEGNFEIANAFFNFLSAKLPISRLQR
                     DLTDSTVIRNIGVPFAHVLIATQSLSKGLSKLVLNDTVIKKDLENNWEVVTEAIQTIL
                     RREGYPEPYEALKSLARTNQPITKKTIHDFIENLDVNENVKEELHKITPFNYTGI"
     gene            9984..>11800
                     /locus_tag="PLR72_02730"
     CDS             9984..>11800
                     /locus_tag="PLR72_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK36158.1"
                     /translation="MFKFFTLLKRFIPPYKKYVVLNLVYNLLATIFSLFSFATIIPIL
                     QILFGIQRANVSYQPWSWNDSLKDIFNALKNNAFYSIEQIVEMSGASTALLYLGLFLI
                     VITFLKTGTAYLASYYMVPIRTGVVRDLRNQLYRKILNLPIGFFTEERKGDIISRMTN
                     DVNEVEASIISSLEMLFKNPIMILIYLIVMFAMSWKLTLFVLLLLPFSGWLIGKIGKS
                     LKQRSTLGQQQTGELLSQIEETLSGLRVIKAFNAEKSFQNKFAQLNEKIRRTFNRINR
                     RYNLAHPLSELVGTIVIAIVLWFGGVLILENHSSINAAEFIYYLIIFYSIITPAKDLS
                     KAGYSIQKGLASLERIDRILTTPNTIKEPASPMPMKPFSDKIEFRNISFRYKTEWVLR
                     DINITIPKGKTIALVGQSGSGKSTLLDLLPRYYDPQKGEILIDGVNIKSLRTSDIRAQ
                     IGNVNQEAILFNDTFFNNIAFGSENVTMEQVIEAAKIANAHDFIMATEKGYETTIGDR
                     GSKLSGGQRQRVSIARAILKNPPILILDEATSSLDTESERMVQEAIEKLMQNRTTLVV
                     AHRLSTIKRADEICVIYEGRIVERGKHEELLALNGYYKR"
     assembly_gap    11801..11900
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11901..13022
                     /locus_tag="PLR72_02735"
     CDS             <11901..13022
                     /locus_tag="PLR72_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOK36159.1"
                     /translation="SDYFNNKTIAKFLKFCKIMPAFRMREGIENIDKNNIVFDKCVEI
                     LHHNKVIGIMPEGNQGSQRKIRPLAKGIFRIAFQAQEKYDSQLAVKIVPVGIDWGNID
                     KYGEHVIINIGKPIEVADYMSEYEENPVTAINKIRDELKNRLSELTLDIATNENYNCF
                     ETATKVANAAMVEKLQLPNKEPFLFFARQKTAQRLLELEQTHPELVEKLTMLCKEYNE
                     NLKKLNLPSWLFNKTIKTSSLVLESLLLLVSLPLFLIGFILNLLPFFTPDLIIKAFHL
                     NFPGGRGSLRFGIGLVTFPLFYILQAILFYKKLKATWWATILFFPLQYYFGKFAFFWH
                     KEFKKLKAKIQYLTLKVRQSIVLERTKFLHDQIIHLIEI"
BASE COUNT         4426 a         2250 c         1978 g         4433 t
ORIGIN      
        1 aaaagaaata ttcaaatggc acagagccag aagaatatca atttgaagtc gtatggtcta
       61 tagaacatga tttaattatt gaagaagaag caaatcaaaa gaaaacatca ttattaaaag
      121 cccttgacaa actcacaccc cgtcaaaaag aagttattta ccttcgtttt acaaaagaac
      181 tagattactg ttctatagct gaaattatga atattagtat agagtcttgt cgcaatctta
      241 tctcaaaagc tattttcaat ataaaaaaag gtaaacggga aaaaataaaa ttcgcgataa
      301 atatttttta cttaagtcca aacggaagga atattagtga agtagttttt gatgctatct
      361 tattatcagt cataatgtaa tttaatgatt tccttatttc taaatcttat ctatccaata
      421 aggttatttt tcaaatttgc tctgcatttt agtagcggga acgagagttg aactcgtgac
      481 ctccgggtta tgaatccgac gctctaacct actgagctat cccgccttct gtttttgcag
      541 tgcaaagata agctttttga aaaaattaga caaaactttg atttgaataa gctaagatta
      601 tttgtaaaaa aactatataa taaaaatgaa tttttaaaag atttattctc tctttgaaat
      661 atagaaatga atccttttga aaatagtaaa taaaaaataa acaccctatt ttttttcttg
      721 attattaatt aatgatggca ttaatcccat ttctttaccg attttcatca tatattctcg
      781 agctgcttca tattcattgg gaataacgcc atccaaaatg gcatctttaa ttcgctgctt
      841 tatttctcct accgtagcac aggctggcaa attgaatgtt ttcataattt catttccatc
      901 aataggtggc tgaaaattac gaattcgatc tttttcttca atttctttca gtttttttcg
      961 taccaactga aaattatttc tatattgctt aactttttcc gggttttttg aagttatatc
     1021 cgcttcacaa agcatcatca aatcatcaat atcattacct gcttcaaaaa gtaatctcct
     1081 gacggctgaa tcagtaactt cttcttcact taacacaata ggacgcatgt gtaaaaaaac
     1141 taatttttga acatatttca ttttttcatt gacgggtaac cgcatttttc tgaaaatatc
     1201 ggctaccatt tttgaaccta caacattatg attgtaaaaa gtccagccag tctgtttgtc
     1261 aaaacatttt gttagcggtt ttccaatatc atgcaaaaga gctgcccatc gcagccacag
     1321 cgaatctgtc tgaaaactta aattatctaa tacggcaagt gtatgataaa aaatatcctt
     1381 atgtccgatt ccatctttgg tttccactcc ttttagagca aaaagttcag gaaaaataag
     1441 ttcaagcagt cctgactgat cgagcaaaat aaaaccaatg gatggttttt ttgataaaat
     1501 aattttgttt aattcatcgg taattctttc ttttgaaata atttctattc gttttttatt
     1561 tcggcgaatc gcttcaaaag tattcacttc cacataaaaa ttcagctctg ttgcaaaccg
     1621 aatacatctt aacattcgta aaggatcatc agaaaaagta atatcaggat ccaatggagt
     1681 tcttagcact gaattctgca aatctctata tccgttaaat ggatctacta attcaccaaa
     1741 acgattttta ttcaagcata atgccaacgt atttatggta aagtcacgac gtttttgatc
     1801 gtcttccagt gttccatttt ccacaatggg ttttcgtgaa tctgagcgat acgattcttt
     1861 acgagctcct acaaattcaa tttctaaatc gtttgttttt acttgtgctg ttccaaaatt
     1921 tttaaaaaca gtcaattttg cttttttatc caatttttca actactcgtt ttgctagctc
     1981 aatgccactt ccaatcacca caatatcaat atctttcgac gatcttttca aaaaaatatc
     2041 gcgtacaaaa ccaccaataa tgtaacattc ttcttgttgt tcatcggcag tttcggaaat
     2101 aatcttaaat attggatgct gtaaatattc ttttatagat tcattattcc attcttgcaa
     2161 atccaccatt gattttgata aattgattca ctatttctcg ttaaaaacca gataaataat
     2221 tatttttgtg ataataagat taccaagtgc aaaagtagag caaaaattaa aaataaaaaa
     2281 attaagaaaa atcttgttgt actgaataaa tgaatggtta caatttgagg atctaaaact
     2341 aaaaataagc atgatgaaac aaaagaaata tggtgctttt taaagtttca tcccattatg
     2401 aatgtatttt ccaatgaagc taaagacgct ttgttaaagg agcaaaaata tcttaatttc
     2461 taatgagctt ccagccagtt attgcctatt ccacaatcca caatcagcgg tacgtttaat
     2521 tgaaaagcat gttccatttc gtaaatcaca agttctcgca ctgtttcaac ttcttctttt
     2581 ggtacagtaa agtctaattc atcatgcact tgaataatca tttttgtttg taaattttcc
     2641 tgctcaagtc ggtgttgaat gttgaccata gctattttaa taatatcagc agcagatcct
     2701 tgaataggag catttatagc atttcgctcg gcaaagcctc tcactacact gttttgggaa
     2761 tttatgtcag gtaaaaatcg tttccttccc aacaaggttt ctacgtaacc tttttctcta
     2821 gctttttgaa taatattatc tatataactt tttaccccag gataagtatt aaagtattca
     2881 tcgataaaat ttttagcttc tgtccgtgaa atacctaatc gttcagccag accaaaagct
     2941 gatattccat aaatgatacc aaaattagca gtttttgcat ttcgcctcat ttcaggagta
     3001 acttcttcaa tagggacttt gtaaattttc gatgctgttg cagcatgaat atcctgacct
     3061 tttttaaaag cctctaccat tgcctcatca ttgcttaaat gagccatgat gcgtaattca
     3121 atttgtgaat agtcggcact aaaaaaaagg ctattttctt cgggaataaa agctcttcta
     3181 atttctttac caatatcatc gcgaatagga atattttgca aattgggatt gctggaactt
     3241 agtctgccag tggctgtatt aatttgatta taagtagtat gtattttacc tgtttgaggg
     3301 ttgattaatt tgggaagagc gtcgatataa gtacttaaca gttttttaat gtttctgtat
     3361 tccaatatct tacctactac tggatgctta ttacgcaatt tttcaagtat atcttcagaa
     3421 gtagaatatt gacctgtctt tgtttttttg gcattgtcat ctaaacgcaa atgttcaaac
     3481 agtacctctc caacttggct gggtgaacta acattaaatt caaaaccaac catctgataa
     3541 atttcttttt ctattttcaa catttcattg gttagaattt ttgaactttg ctgtaacact
     3601 tctgtatcaa tacgaacacc tgtttgttcc ataatggcaa gaactcgcat caaaggcatt
     3661 tcaatatcgt aaaaaagttt ttccagacca ttttctaaaa tttctttttc taaaatctgt
     3721 tttaatcgaa aagtaatatc tgcatcttcg caagcatatt cacaaagttt ggctatttca
     3781 atatttcgta tatctgcttg atttttccct tttggtccca ctaaatcttc ataatgaata
     3841 gtacgataat gcaaataaat ttcagcaaga taatccatac tgtggcgtaa ttctggttgc
     3901 agaagataat gagcaatcat ggtatcgaaa agctttcccc tcagttcgat attatacata
     3961 gaaagcatca aataatcgaa ctttaagttc tgtccgattt tttctatctg ttcactttga
     4021 aaaaaagctt taaattcctt taaaatgatc gccgcttctt ttctgtcagt tggtaaagta
     4081 acaaaataag cctccccttc cttgaagcag aaagaaaagc ctaccaaatc agcattaaac
     4141 acatcaaggc tggtggtttc tgtatcaaag caaactgatt tttgtgtaaa aagttgagag
     4201 ataagataag cacgctttgc tttggtatca accaattgat aacgatgagg aacattcctc
     4261 aatgtcagat atccatccgg actaatttct tcaccaacag tgatcttttc attttcagat
     4321 ttttcctttg tttctgaaag agtttgcgga gtttccgtac caaacaaaaa catttgctga
     4381 ccttccttat tcttcttcgt ttcgtcaaca ttttttgcag acacatcggt cgggaataaa
     4441 atagttgaat taccaaattt attagctaac agcgtacgaa attcaagttc tttaaataaa
     4501 gctcgcaatt tggtttcatt tacgggagaa attttaaatt tttcttcatc aaaagaaaca
     4561 ggaacatccg ttttaatagt tgctaaaaat cttgaaaaaa gaataagatc tttattttct
     4621 tcaattttct gtcgcaaagc acctttcaat ttgtctgtat ttttcaataa attatcaata
     4681 ctgccaaatt cctggagcaa ttttatagcg gttttttcgc caatacccgg acaacctgga
     4741 atattatctg agctatcgcc catcaatcct aacaaatcta ttacttgctg ctgactatta
     4801 agctgatatt ttgccttaat ttcttcgggt ccaagtattt caaaaccacc attgtgatga
     4861 ggacgataca taaatatatg gtcagaaacc aattgcccaa aatctttatc acctgtcagt
     4921 aaataaacat catagccagc tttttctgct tgttttgcaa tggttcctat tacatcatca
     4981 gcttcataac caggaacttc cagaatggga atattataag catccaccaa ttcctttacg
     5041 ataggtattg cctttcgaat atcttccggt gtcatttcac gctgtgcttt gtattgctca
     5101 aaagcttcgt gacgaaaggt cttaccttca ggatcaaaag ccactgccat atgagtgggt
     5161 tgttcttttt ttaaaacttc ttccagggta tttacaaaac caaaaattgc tgaagtattc
     5221 tgaccttttg aattaatacg tggattgcga ataaaagcat aatacgaacg ataaataaga
     5281 gcatgagcat cgactaaaaa aagttttttc ttttccattg atattcaaat attgtaagta
     5341 taacacttct agaaaagcaa atgctaataa aaattgcttt ttgatgattc attttgtttg
     5401 taaaattaat taaaaaaatc tcagaaatga atattttatg gtaaaacaga cattgaaatt
     5461 gattattttt gtagacattt caataaatta ctcccatgaa tgtaatagaa attattaaaa
     5521 aacctattat caaggaacta cattcatttg aaaaacaact tgttaatgaa ttgaaaacag
     5581 agaatccttt gttaactaac atcaatgaat atattcaaca aggaaaaggg aaacaattgc
     5641 gtcctatttt aatgttttta tctgctaaaa tttgtggagc tattaatgaa caaacaatta
     5701 tttcagctat ttcaatagag ttgttacata ctgccagttt aattcatgat gatgtagtcg
     5761 acaacgcttt tgaacgacgc ggaagaccgt cagtaaatgc tcgttgggat aataaaattg
     5821 ctattttagc aggtgattat ctgttatcaa aatcattgaa ttgtgctata aaagcaaata
     5881 atctggaaat tttacatgtt attgccaaaa tcggggtgga attaaccgat ggcgaattat
     5941 tacaattaga gaacatgcaa aaaattaaga tcacagaaga tgattattat aaaataattc
     6001 gaaaaaaaac tgctctttta ttttcttctt gctgcataat tggaggtcta tcagtaaatg
     6061 ccaaccaaac gctcttaaaa aaattaaaaa attttgggga atatgtagga atttgtttcc
     6121 aaattaaaga tgatattttt gactactctg atagcaaaaa cattggtaag ccaagaagga
     6181 atgatattag agaaggaaaa ataacccttc ccttaattta tgctttacag caagccgacg
     6241 aaaagaaaaa agaaaaaatt atcaactgga taaagaagaa taaattttct gaaaagaaca
     6301 tagaaaaaat tacgaatttt gtttataaaa acggtggcat tgagtatgct acttcaaaac
     6361 tgattgaatt taaaaataaa gctctcaccg aacttgatag ctttgaagaa agtgaaataa
     6421 aacaatcatt gaaatatttt gctgaatata tggttacacg tgaggaataa ttaaaagtat
     6481 tttatttctt ttccttctat ggaactgagc aattgattgg ctaaacggct tgctccaaaa
     6541 cgaaaccatt gattattaag ccattcgttg ccaagaatat gttttacgag cgtaaaatgt
     6601 ttcacagctt cttcggtagt tgaaattcca cctgctggtt tataacctat tttacttccg
     6661 gttttcccat accattcttt gatcgcatga caaatagtat aagtagcttc gagtgtagct
     6721 gaaacctgaa tttttcctgt agaagtttta acaaaatcag ctcccgatgc catcgctaat
     6781 aaagcagcct tttttatatt cgtacaattt ggtaaagccc ctgtttcaag aatcactttt
     6841 aaatgagcat cccgacaggt tgcttttatt tctgataact catcataaac ttcttcatat
     6901 ttttcttcaa gaaattttcc tacagaaatt accatatcta tttcgtttgc acctgcttga
     6961 acagccattg aagcttcagc aattttcact tcaagaaaag tttgagaagc aggaaaacca
     7021 ccagctactg atgctatttt cactttttga tcattcaaat gcttttttac aacaggtact
     7081 aatgctggat aaacgcaaat ggcagctaca ttggataaat gcgggaaaac atttttaaaa
     7141 ttatttacct tttctgtcaa cgtagcaatt tcactttctg tatcggtaca atttaacgaa
     7201 gttaaatcta tcagatttaa acattgatta taaacttcct tgttagcatt ttcagcaaaa
     7261 tgttcagcca atattttatc aatctcaatt tttacagctt catcattgac ttcacaatgg
     7321 taaaaatcaa ataattcctg atacttgtct ttcataataa attgattttt aaatatttaa
     7381 tgcaactttt tattttgctg atgccgttga gcatctcttt ctgttttctt tttaatattt
     7441 ttttcaaaag cctcagtaag gtcaattcct gtttgattgg cgagacaaat caatacccac
     7501 aatacatctg ccatttcatc agataaattt gttttttctt cttctgcttt cgatgattgt
     7561 tcgccataag tacgggcaat aatacgagcg agttctccga cttcttcggt tagaagaacc
     7621 atgttggtta attcgctaaa ataacgaacg ccatattttt ttatccaatt atcaacttgc
     7681 tgttgagctt ccttgattgt catctttatt ttcttttaaa cgagcaatga tagtttcatt
     7741 tcctgttgtt ttttctccta cctttacaaa tatttccgta tccagaggta aaaataaatc
     7801 tactcgtgaa ccaaatttta taaatcccag atgttcattg atatggcact ctttccccgg
     7861 ataagcataa gtaacaattc gctgagctaa agcaccagcc acttgtctta ctaaaatctg
     7921 ggtttttaat ggagtttcta ttacaataga tgcttgttca ttttctttgc tcgattttgg
     7981 caaataagct gccaaatgac gaccaccacg atgcgatgtt ttaattactt tccctgaaac
     8041 aggataccag ttggcatgta cattaaaaat ggacataaaa atagaaactt gtatcctttc
     8101 atcgccaaaa tattctgttt ccagagtagg ttctatcact accactgttc catcagccgg
     8161 agcaactacc aaacttggat catcaatttc aaatatacgc ttcggacttc gaaaaaaaaa
     8221 tgtaaaaaaa cacaaaacca atgctgaaag aatggtatta atagcaacaa ctgttactgg
     8281 aaatttcaga taaataacaa ggttaatgca aaaaattaac agcaacagtc ccgataaaat
     8341 aaaacgtcct tccttatgta tattaatgcg tttcattcag aataagtgaa aaatatctaa
     8401 ctctcaataa atttttatga ttaaaaatcg taaaaatgaa gcctacaaaa gtaatgaaaa
     8461 agacgcttgg ttgcaaggca aaaataaaac tttccttatt aaacagaatt gattaacttt
     8521 gcaagtaata acatattagc ttaattaaat tttttcattt cttacaatcc tgatatctta
     8581 atttattaaa ctaagaaaca tttatgaatc tttctccact gacagccatt tcaccaattg
     8641 atggacgcta tcattccaaa acgaagatgt atgcttcgta tttttctgaa ttcgccttga
     8701 tacgttgcag agtgcagatt gaaattgaat attttatagc cttatgtgaa ataccattag
     8761 aacaattaaa acagatttct gctgatattt ttccaatgct cagaaatatt tatcaaaatt
     8821 ttcaggaaac cgatgcacaa aaaataaaag atattgaaca aattactaat cacgatgtaa
     8881 aagcagtaga atatttttta aagaaaaagt ttgaggaaat agggttaagt aaatacaagg
     8941 aatttattca ttttggactc acttcacaag acatcaataa cacagctttc ccatttcttt
     9001 tgaaagctgc ccttgaagat gtttattatg ctgaattgaa aaaattgctt gaagagttgg
     9061 atcaattagc tgaaaactgg aaagatattc caatgctggc aagaacacac ggacaaccag
     9121 ccacaccaac ccgattagga aaagaaatca tggttttttc ctatcgtatc aaagaacaat
     9181 tagaactttt aaaacaaatt cctatttcag ccaaatttgg tggagcaact ggaaatttta
     9241 atgctcatgc tgtgtcttat ccagccattg actggaaaaa atttgcggat aattttttgt
     9301 ctgataaatt tggcttgaaa cgagaacaat acaccacaca gatttcaaat tatgaccatt
     9361 tatctgctct gctggatgca acaaaacgaa tcaatacggt tttgatagat ttgtgccgtg
     9421 atatgtggac gtacatttct ctggaatatt ttaaacaaaa aatcaaagca aatgaagttg
     9481 gttcatcgac catgccccat aaagtcaatc caattgattt tgaaaatgcc gaagggaatt
     9541 ttgaaatagc taatgctttt tttaacttct tatcagctaa attacccatt tcacgtttac
     9601 aacgtgactt aaccgacagt accgttattc gtaacatagg cgtacctttt gcacatgttt
     9661 taatagctac tcaaagcctt tcaaagggat tgagcaaatt agtattaaac gacacagtta
     9721 taaaaaagga tttggaaaac aactgggaag ttgttactga ggcaatacaa actattcttc
     9781 gtcgcgaagg ctatcctgaa ccttatgaag ctttaaaatc cttagctcgt actaatcagc
     9841 caattaccaa aaaaactatt catgatttta tagaaaatct tgatgtcaac gaaaacgtga
     9901 aagaagagtt acataaaatt actcctttta attacactgg gatttgattt ttttgaatta
     9961 ttaaaattca ttattcttgc caaatgttta aattttttac actcctgaaa cgatttattc
    10021 caccttataa aaaatatgtt gtattaaatc tcgtatataa tttattggct actattttta
    10081 gcttattttc atttgctact atcattccta ttttgcaaat tttgtttggt atccaacgag
    10141 caaatgtaag ctatcaaccg tggagctgga atgatagttt aaaagatatt ttcaatgcat
    10201 taaaaaataa tgctttttat tccattgaac aaattgttga gatgtcagga gcttctactg
    10261 ctttacttta tttgggattg tttttgatag ttattacctt tttaaaaact ggaaccgctt
    10321 atctggcttc ttattatatg gtacctattc gaactggtgt ggtacgagat ttacgcaatc
    10381 aactttacag aaaaattctc aacttgccaa ttggcttttt tacagaggaa cggaaaggtg
    10441 atatcatttc ccgtatgacc aatgatgtta atgaagtgga agcatctatc atcagctcat
    10501 tggaaatgct tttcaaaaat cccattatga ttttgattta cttgattgta atgtttgcta
    10561 tgagttggaa gctaacactt tttgtactgc ttttattacc gtttagtggt tggttaatag
    10621 gcaaaattgg taaatctttg aagcaacgtt ccactttagg acaacaacaa acgggcgagt
    10681 tactctcaca aattgaagaa actctgagcg gattacgtgt gataaaagct ttcaatgctg
    10741 aaaaaagttt ccaaaataaa tttgcacaat taaatgaaaa aatacgtcgc acatttaatc
    10801 gaattaatcg tcgttacaat ctggctcatc ctttaagcga attagtagga accatagtga
    10861 tagccattgt attatggttt ggtggcgtat taattttaga aaatcatagt tccatcaatg
    10921 ctgcagaatt tatttattat ctgattattt tttatagcat tattacacca gcaaaagatt
    10981 tatcaaaagc aggatatagt atccaaaaag gattagcttc gttggaaaga attgatagaa
    11041 tcttaactac acccaacacg attaaagaac cagcttctcc tatgccaatg aaaccattta
    11101 gtgataaaat tgaatttcga aatataagtt ttcgatacaa aactgagtgg gtgctgcgtg
    11161 atattaatat taccattcct aaaggaaaaa ccattgcact ggtaggtcaa tctggttcag
    11221 ggaagtcaac tctcttagac ttattaccaa gatattatga tccacaaaaa ggtgaaattt
    11281 taattgatgg agttaatatc aagtcgctac gaacttctga tattcgtgct caaattggaa
    11341 atgtaaatca agaagctatt ctttttaacg atactttttt caataatatt gcctttggct
    11401 ccgaaaatgt aacaatggaa caagtaatag aagctgctaa aatagcaaat gctcacgact
    11461 tcattatggc gaccgaaaaa ggatatgaaa ccaccatcgg tgatcggggt agtaaacttt
    11521 caggaggaca acgacaacgc gtcagcattg ctcgtgctat tttaaaaaat ccacctattc
    11581 ttattcttga tgaagccact tcttcgctcg acactgaatc tgaaagaatg gttcaagaag
    11641 ccattgaaaa attgatgcaa aaccgaacaa ctctagtagt agctcatcgt ctttccacta
    11701 tcaaacgagc cgatgaaatt tgtgtgatct atgaaggacg catcgtagaa cgaggaaaac
    11761 atgaagaatt attggctttg aatggttatt ataaacgatt nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn tctgactatt tcaacaataa aaccattgca aaattcctca
    11941 aattttgtaa aataatgcca gcttttcgca tgcgggaagg aattgaaaat atagacaaaa
    12001 acaatatcgt ttttgataaa tgtgtagaaa ttctgcatca taataaagtc attggtatca
    12061 tgccagaagg caaccaggga agccagcgaa aaatcagacc tcttgcaaaa gggattttta
    12121 gaattgcttt tcaagctcaa gaaaaatatg attctcaatt agcagttaaa attgtacctg
    12181 ttggtataga ttggggaaac attgacaaat atggcgaaca cgttatcatt aacattggaa
    12241 agcccataga agtagcagat tacatgagcg aatatgaaga aaatccggtc acagcaataa
    12301 ataaaatcag agatgaatta aaaaatcgtt taagcgaatt gacacttgac attgctacca
    12361 acgaaaacta caattgtttt gaaactgcta ccaaagtggc taacgcagct atggttgaaa
    12421 aattgcaatt accaaataag gaaccctttc tgttttttgc ccgacaaaaa acagctcaac
    12481 gtttacttga attggaacaa actcatcctg aattagttga aaaattaacc atgctttgta
    12541 aagaatacaa cgaaaattta aaaaaattaa acttaccttc atggctattc aacaaaacta
    12601 taaaaacctc ttctttagtg ctggaaagtt tgttactctt agtttcttta ccacttttct
    12661 taataggttt tatactgaat ttattgcctt ttttcacccc tgatttaatt attaaggcat
    12721 ttcaccttaa ttttcctggg ggtcgtggct cattacgatt cggaatagga ctggttactt
    12781 ttcctttgtt ttatatttta caagccattt tattttataa aaaacttaaa gctacgtggt
    12841 gggcaactat tttatttttt cctttacaat attattttgg aaaatttgct tttttctggc
    12901 ataaagaatt taaaaagtta aaagctaaaa ttcaatatct tacacttaaa gtacgacagt
    12961 ccatcgtttt agaacgaacc aaatttttac atgaccaaat cattcatttg attgaaatat
    13021 aacgataaat tttttttata aaaagcctta tctaaatttt ttaattcaaa taaggcttta
    13081 aagtaagact aaaaaaattt aaactgaaga gtccttttct tgcgaagaag aaaaaagatt
    13141 ttttatttct gaaaaacttt gttttaaagt ttgcgatatt tctttaa
//
LOCUS       DAOSAS010000035        13099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3214_length_13099_cov_13.882168, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000035 DAOSAS010000000
VERSION     DAOSAS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13099
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3214_length_13099_cov_13.882168"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLR72_02740"
     CDS             <1..772
                     /locus_tag="PLR72_02740"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOK36160.1"
                     /translation="KLDPINFNLLSPYTIQKMIKGVEILRKLQKISGETTEIYTYQNC
                     TIKKLALLKGIDLYKMGIYKFLGNSLISRLNEKEFSNIEEIRQYLIPDTQTGRGEWID
                     LAGLIAPKSEVENLIKKIEVGTINLSEIQLEFEKMHQEYYTYEWTWAKEKLEIHWGKP
                     IEEVTIDEIISMIELWKKSVITLDELIYEDAKKEFSLNAKTGFGVDGDEEQKNLDFES
                     VRGSFENNPFVLEVINHIKIKRELGDGLISRLEKCRVN"
     gene            complement(943..1015)
                     /locus_tag="PLR72_02745"
     tRNA            complement(943..1015)
                     /locus_tag="PLR72_02745"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(980..982),aa:Lys,seq:ttt)
     gene            1180..2427
                     /locus_tag="PLR72_02750"
     CDS             1180..2427
                     /locus_tag="PLR72_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOK36161.1"
                     /translation="METFPAILLTLLFSAFFSGMEIAFVSSNKLRFELDKKQRSLSSY
                     FLSIFYEHPNQYISTMLVGNNIALVIYGLLMAKILNPLFEPIGNQFIITFLQTIIATA
                     IVLITAEFLPKTIFRINSYLWLRVFSGILFFFYVVLYPITYFVTWLSFSVLHLFGVKT
                     SDASREKVFSRADLNYLIQESFENKNDEKEINKEVEIFQNVLDFSNVKLRDCLVPRTE
                     IVALDYEVDEETLKQTFIETGFSKIPIYKGNIDNIIGYIHSSEMFVHPKDWRDHLNQI
                     PIVPENMSAQKLLKIFLKEKKTIAVVIDEFGGTAGIVTLEDIIEEIFGEIEDEHDTHE
                     YTCEQLNEKEYLFSGRLEIKEINEKFNLNIPESDNYETIAGFILYHLERFPKLNEVIQ
                     IDKFTLKCIEIKNNRIELVKLSL"
     gene            2587..4701
                     /locus_tag="PLR72_02755"
     CDS             2587..4701
                     /locus_tag="PLR72_02755"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOK36162.1"
                     /translation="MATLGKIRSKGILLVVVVGLALLAFIIGDFLNSSTSYFNKSREK
                     VAQIAGENINIRQFSEAIEQMTQVYKIETGQTELNEDIISQIRASVWESLVNEIILNK
                     EAKKLGLTVSPEELSDQLIGYNIHPLILQRRVFADENGQFSRSLLIQFLNSFDATPTS
                     NEMKQQIDMAKNYWLFWEKNVKNSILQEKYNKLITSAVTANGVDAKMNYEARKFTVDS
                     WYVVQPYFAVPDSLVTVTASEIRARYNKQKEFFKQEENCSIDYVVFDIKPSPEDFKEV
                     EQWINRLSEEFRTTSDVIGLVNSNSDIIYDGRPYSEQTIPPMLKDFAFKGKTGDVFGP
                     VFENDTYIMARIMETGILQPDSVKLRHIFLPKSEEAKADSIIKAIRSGADFALLAKKY
                     SAVPQTAANGGEIGWLTYDMKGIDKEILENAFNKPLNEVFTIKNAQGTQIMQVTAKTT
                     PKKKVKVAILQRKVTPSSKTYSLIYNQAKQFAAENNAELFAKRAQEKGYIVHHAERVL
                     KNDNQLHSISNSRQIIRWAFQSPKGSVSDVFDCENQYVVAMVTEKNQKGYQPIEKVSA
                     QLKSEIIKDKKADLMIKNISAQLKKTPTLEDLASTLGTEVKTAEAINFTSYQFGLAGF
                     EPYIIGKATVIPLDKISGPYKGNAGVYVLKASNKQESQEPFNVKNEISQLNSRYLYSL
                     FYAIIDDLREKSEIIDNRSNFY"
     gene            4883..5923
                     /gene="rlmN"
                     /locus_tag="PLR72_02760"
     CDS             4883..5923
                     /gene="rlmN"
                     /locus_tag="PLR72_02760"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOK36163.1"
                     /translation="MNKKSLIGLTLEELKTIVTDFGMPSYTAKQIAEWLYKKRVLSIN
                     EMTNISSKNRALLNEKYKVGRSEPLQVTISKDGTKKYLFATENNHFIETVLIPEENRV
                     TLCVSSQAGCKMNCLFCMTGKQGFVSKLSAGEILNQILSVPEASEITNLVFMGMGEPF
                     DNTIAVLTALEILTADYGYAWSPHRITVSTIGLIPGMKLFLEKTNCHLAISLHSPFHE
                     ERLQLIPAEKAYPIKKILEELKKYDFSKQRRLSFEYIMFDHFNDTMRHARELVKLLKE
                     IPCRVNLIRFHAIPDVPLKTSSEEKILSFRNYLNENGIIATIRKSRGEDIFAACGMLS
                     TSAKEKETKNQL"
     gene            5930..9010
                     /locus_tag="PLR72_02765"
     CDS             5930..9010
                     /locus_tag="PLR72_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOK36164.1"
                     /translation="MKKSINIFFKKNWLIFFLLFFGVNICNIQAQQFLSTISDSLTAI
                     ANEETYIKRIAIKDFSVTQKPKKVIITADERLSYIPFRPENVERIYNALEKILMPTYE
                     GYKIVCQVDNKPIEELIPNFYRTGKKDKSRIFITKKIEFPLVKNLSRPYEITRGLQNK
                     HIAVWPSHGYYYNQNLARWQWQRGRLFQTVEDIYTLSYIYPYLVPMLENAGANVLIPR
                     ERDVQKNEIIVDNDMSDDLSKYSEINQSFQWKNGQDTGFANQKNNYLDGENPFMMGTY
                     RQILSVNNKDKVSSVEWIPFIPQSGKYAVYVSYKTLTKSTSTAHYTVYHKGGQTEFLV
                     NQKMGGGTWIYLGTFYFNEGCNEDSKVVLTNLSSKKNEIITADAVKFGGGIGNIARQP
                     LAEGLITNLKSSEELIIEKNKISDKNFHQDTIHEPEISGYPRFTEGSRYWLQWAGFPD
                     SVYSFTKRKNDYIDDYQSRGLWVNYLIGGSPLAPSDKGLGIPIDLSLAFHTDAGTTSN
                     DSIIGTLGIYTVLNSYQKDIFKNKISRWTSRDLTDIIQTQIVTDIRNMYAPEWTRRRI
                     WNKNYSESRLPEVPSMLLELLSHQNFADMRYGLDPRFQFLVSRSIYKGILKYLHSDNP
                     NKYCVQPLPVKQFSVQFIDTTTLKLRWTNEIDFLEPTAVPEYFVVYIRRDENDFDNGI
                     LVKNNEYTVNIEPGKIYSFKVTAVNKGGESFPSEILSAYRAPDEKGEVLIVNGFTRLS
                     APESFSIDTLYAGFVNDKDAGVPYLYQYNFTGKQYEFSKNKLWVNDDAPGFGASYANY
                     EGKILVGNTFDYPFLHGKAIKEAGYSFSSCSSDAIINEEINLNDYKMVDLILGKQKQT
                     YIGNGKKSTAFKTFPLDLQRKINLFCQNGGNLFVSGANIVSDLCDNPLTLPEERSFLE
                     NTLKLKYCTHQAAVTGEVKFINSPLPFFKKINFSYFHEPNEFCYFVESPDAFEPNDKK
                     AYTICRYSENNLSAAVAYDGNYKVCAFGFPFETIISENDRQNIMESILSFFTKH"
     gene            9308..10339
                     /locus_tag="PLR72_02770"
     CDS             9308..10339
                     /locus_tag="PLR72_02770"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOK36165.1"
                     /translation="MKNKILILLSAIFFLLGCQKTKTSLPVATGTMFEVIVVMDEPAW
                     SSLPGNTLRETLDQPMEALPQPEPIIDMHHCQHQEFSNMYKSSRNILITEISEKHVQP
                     KVIYTKDKWASPQAIVQITAPNDSSFANTVRQYGNKILDYYVITERERFIEFNKNHLN
                     VKAKTEIEQMFGTQIDVPSFLTKATKKKDFYWLTNDNPKIRQDLVIYSYPYTDKNTFT
                     KEYLLAKRDTVMKTNIPGEIQGSYMGTEYKNYPPIFREIWVNGGYCAEIKGLWKMING
                     AAMGGPFYSHTRLDEINQKVITVEGFVFAPGEKKRNAIRQLEAVIYTTKLPQEINALK
                     EVSVTPKNN"
     gene            10360..11430
                     /gene="pdxA"
                     /locus_tag="PLR72_02775"
     CDS             10360..11430
                     /gene="pdxA"
                     /locus_tag="PLR72_02775"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOK36166.1"
                     /translation="MDKIIIGITQGDINGIGYEVILKSLSDAAIFDLFTPVIYGSSKI
                     AAYHRKVLNIQNLSFNNINSVEEANPKRINIINCTSDEVKVELGQPTAESGKAARDAL
                     ERACSDLGKGLIHALVTAPINKKNIQSEKFHFPGHTEYLAEKFGTNKAPLMMLINDFM
                     RIAVVTGHIPLQKVASTLTTELILEKIETLNDSLKEDFLIVRPRIAVLGLNPHAGDEG
                     VIGDEEEQVIIPALKAASEKGIICLGPYPADGFFGSGNFSNFDGVLAMYHDQGLIPFK
                     TLSVESGVNFTAGLSIVRTSPAHGTAYDIAGKNLASENSFREALYMACDIYKNRTWNN
                     EIKSNPLKTIPKSENETNIQVE"
     gene            11439..12059
                     /locus_tag="PLR72_02780"
     CDS             11439..12059
                     /locus_tag="PLR72_02780"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOK36167.1"
                     /translation="MNKTQQEELFYGCQSTIFGDCCLVFTRQNILRLFFTDEREKTLQ
                     YFEQLTEKMNLKRYDEKAKELIDQIFIERKTFPLVLKGTAFQLSVWKALQTIPFGETT
                     TYKVIAEAIGRPNAIRAVGNAVGANSIAFLIPCHRVIRSDGQLGGYRWGVERKQKMLE
                     WEKINFKNKFIYSAIFSLNPDNYQCVCREKMARFGELVHFLPNQYL"
     gene            complement(11947..12825)
                     /locus_tag="PLR72_02785"
     CDS             complement(11947..12825)
                     /locus_tag="PLR72_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HOK36168.1"
                     /translation="MKVALIQQSNGKDTSANLDKLQENIRKCAAEGAELVVLQELHNS
                     LYFCQTENPDYFGLAETIPGPSTDYFGKLARELGVVLVLSLFEKRAAGLYHNTAAVLE
                     KDGTIAGIYRKMHIPDDPAYYEKFYFTPGDLGFVPIQTSLGKLGVMVCWDQWFPEAAR
                     LMALAGAELLIYPTAIGWETTDSEEEKQRQLEAWITIQRSHAIANGLPVVSCNRVGRE
                     DDPSGQTAGIQFWGNSFVAGPQGEILAQASNQKEENLLINIDLARSEQVRRIWPFFRD
                     RRIDSYQDLTKRWLNK"
     gene            complement(13014..>13099)
                     /locus_tag="PLR72_02790"
     CDS             complement(13014..>13099)
                     /locus_tag="PLR72_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOK36169.1"
                     /translation="IGIDCRTLIKQHGSLHCVTMQFYPAKG"
BASE COUNT         4729 a         2020 c         2212 g         4138 t
ORIGIN      
        1 aaagttagat ccaattaatt tcaatctatt gagtccttat actattcaaa aaatgataaa
       61 aggtgtagaa attcttagaa aacttcagaa aatttcaggt gaaacgactg aaatttatac
      121 ttatcaaaat tgtactatta aaaaattggc tttattaaaa ggaatcgacc tttataaaat
      181 gggaatctat aaatttttgg gaaattcatt gatcagtaga ttaaacgaaa aggaatttag
      241 taatatagaa gaaatcagac agtaccttat tcctgatacg caaactggac ggggtgaatg
      301 gattgacctg gcaggtttaa ttgctccaaa aagtgaagtt gaaaacctca ttaaaaaaat
      361 tgaagttgga acaataaatt tgtcggaaat ccagttagaa tttgaaaaaa tgcatcaaga
      421 atattataca tatgaatgga cgtgggcaaa agaaaaactg gaaattcatt ggggaaaacc
      481 tattgaagaa gttactattg atgaaattat aagtatgatt gaattgtgga aaaaatcagt
      541 aattacgttg gatgaattaa tctacgaaga tgccaaaaaa gaattcagcc tgaatgcaaa
      601 aactggtttt ggtgtagatg gcgatgaaga acaaaaaaat cttgattttg aatcagtaag
      661 aggaagtttt gagaataatc ctttcgtcct ggaagttata aatcatataa aaattaagag
      721 ggaattaggt gatggactta tttcacgtct tgaaaaatgt agagtgaatt agaacatcca
      781 gctcattttt caaaaaaatt tatgtatagc atgggaagtt gaaaattttc ttatgctttt
      841 tcgtttcctg aggaaaaacg aaaatagcaa aaacaatgat ttttgtcgaa taactgaaga
      901 atatagtaaa aaaagaaagt tttataagga aagaataact tgtgatctag ctgggattcg
      961 aacccaggac cccatcctta aaagggatgt gctctacctg ctgagctact aaatcttcaa
     1021 aagaagttgc aaagatacag tttttttaaa aaatatcaaa gttaattcaa ttatttctta
     1081 tcagttactt ccttcaaaca caaaattgaa aagaattttg ctttttgatg attcatagta
     1141 taaatattat ctttgtaaat aattaggaaa ttactttgtg tggaaacttt ccctgccatc
     1201 ttattaactt tattgttttc cgccttcttt tcaggaatgg aaattgcttt tgtctcatca
     1261 aataaactac gtttcgaatt agataaaaaa caacgtagtt taagttctta ttttttatcc
     1321 attttttatg aacaccctaa tcaatatatt tctaccatgc tggtaggaaa taatattgct
     1381 cttgtcattt atggattatt aatggcaaaa atattaaacc cactgtttga acctataggt
     1441 aatcaattta taataacttt tttacaaacc attattgcta ctgcaattgt actcattacg
     1501 gctgaatttt tgcccaaaac gattttccgt atcaattctt atttatggtt gcgagtattt
     1561 tcaggcattt tgtttttctt ttatgtcgtt ttgtatccta taacttattt cgtaacatgg
     1621 ttatcatttt cagtacttca tttatttgga gttaaaacca gtgatgcttc ccgagaaaaa
     1681 gtattttcac gggcagactt gaattatttg attcaggaaa gttttgaaaa taaaaatgac
     1741 gaaaaggaga ttaataagga agtagaaatt tttcagaatg tgttagactt ttcaaatgtg
     1801 aaactccgcg attgtttagt gcctcgaact gaaatcgttg cattggatta tgaagttgat
     1861 gaagaaactc tgaaacaaac ttttattgaa acaggcttct caaaaattcc catttataaa
     1921 ggaaatatag ataatattat tggttatatt cattcttctg aaatgtttgt tcatccaaaa
     1981 gactggcgtg atcatctaaa tcaaattcct atcgtaccag aaaacatgtc tgctcaaaaa
     2041 ttgttaaaaa tttttctaaa ggagaaaaaa actattgcag ttgtgattga tgaatttgga
     2101 ggaaccgcgg gtatcgtaac actggaagat attattgagg aaatttttgg agaaatagaa
     2161 gacgaacatg atacacatga atatacgtgt gaacaactga atgaaaaaga atatctgttc
     2221 tcaggcagat tagaaatcaa ggaaattaat gaaaaattta atttaaatat tcctgaatca
     2281 gacaattatg aaacaatagc tggatttatc ctttatcatc tcgaacgttt tcctaaacta
     2341 aacgaagtca ttcaaattga taaatttacc ttaaaatgta tagaaataaa aaataatcga
     2401 attgaattag taaaattatc tctctaaaat tataatattt tgatatttag ataattaatt
     2461 aagcctcaaa tgaattctaa aaaattaagg aaatagaaaa taaaaaagtt gaataatttt
     2521 tttgtatatt cgtgctctca aaatcggcag tagaacaatt tagtgaataa tttttaaatt
     2581 taaaaaatgg caacattagg gaaaatcaga agtaaaggaa ttttactggt tgtagtagta
     2641 gggttagcat tattagcatt tatcattggt gactttttaa attcgagtac ctcttatttt
     2701 aacaaatcac gtgaaaaagt agctcaaatt gcaggagaaa acataaatat tagacaattt
     2761 agtgaggcaa ttgagcaaat gacacaagtg tacaaaattg aaacaggtca aactgaacta
     2821 aatgaagata ttatttcaca aattcgtgct agcgtatggg aaagtttggt aaatgaaata
     2881 attttaaata aggaagcaaa aaaattagga ctcacagtaa gccctgaaga actttctgat
     2941 caattgattg gatataatat acatcctttg attcttcaac ggcgtgtttt tgctgatgaa
     3001 aatggtcaat ttagccggtc gttattgatt caatttttaa atagttttga tgcaactcct
     3061 accagtaacg aaatgaaaca gcaaattgat atggcaaaaa attattggtt gttttgggaa
     3121 aaaaatgtaa aaaatagcat tcttcaagaa aaatacaata aattaataac ttctgcggta
     3181 acagccaatg gtgttgatgc gaaaatgaat tatgaagcaa gaaaattcac tgtcgatagc
     3241 tggtatgtgg tacaacctta ttttgctgtt cccgattcat tagttactgt aacagccagt
     3301 gaaatcagag ctcgttataa taaacaaaaa gaatttttca aacaagaaga aaattgttct
     3361 attgattacg tggtttttga cattaagcca tcacccgaag atttcaaaga agtcgaacaa
     3421 tggataaatc gcttaagtga agaatttaga acaacttctg atgtaatagg cttagttaat
     3481 tcaaattctg atattatcta tgatggtcga ccttattctg aacaaacaat cccacctatg
     3541 ttaaaagatt ttgcttttaa aggtaaaaca ggtgatgttt ttggacctgt ttttgaaaat
     3601 gatacttata ttatggctcg cattatggaa actggcattc tgcaacccga ttctgtaaaa
     3661 cttcgtcata tctttttacc aaaaagtgaa gaagcaaaag ccgacagtat tataaaagcc
     3721 attcgctcag gagcagattt cgctttatta gcaaaaaaat attcagctgt tcctcaaacg
     3781 gcagccaatg gtggcgaaat tgggtggttg acttatgata tgaaaggaat tgataaagaa
     3841 atattggaaa atgcttttaa taaaccttta aatgaagtat tcactatcaa aaatgctcaa
     3901 ggtactcaaa taatgcaagt tacggctaaa accactccca aaaaaaaggt aaaagtggct
     3961 attctccaac gtaaagtcac cccaagcagt aaaacttata gcctaattta taatcaagca
     4021 aaacaatttg cagccgaaaa taacgcagaa ttatttgcaa aaagagctca ggagaaaggt
     4081 tatattgttc atcatgctga aagggttctt aaaaatgata atcagcttca ctctatttct
     4141 aattcgcgtc aaattattcg atgggccttc caatcaccca aaggaagcgt atcagatgtg
     4201 tttgattgtg agaatcaata tgtagtagca atggtcacag aaaaaaatca aaagggttat
     4261 caacctattg aaaaagtttc agctcaatta aaaagtgaaa tcattaaaga taaaaaagct
     4321 gatctgatga tcaaaaatat atcagcacaa ttaaagaaaa cccctacact ggaggatttg
     4381 gcttctactc tcggtacaga agtaaaaaca gccgaagcaa ttaattttac ttcctatcaa
     4441 tttggattag ctggtttcga accttatatc ataggaaaag ctactgtcat tccattagat
     4501 aaaatttcag gtccttataa aggaaatgca ggcgtttatg ttcttaaagc ttctaacaaa
     4561 caagaaagcc aagaaccttt taatgtaaaa aatgaaattt cacaattgaa ttctcgttac
     4621 ctctattctt tattttatgc tattatagat gacttgcgag aaaaatcaga aattattgat
     4681 aatcgttcta atttctatta ataaaatatt tcagttctta attaaacgga atggaaaata
     4741 cttcattccg tttatttttt ataattttga tactcgttgc caaataacat aaaaaaatta
     4801 cgaaaacttt ttaaagcgaa taaatgattg aatttgaaag ttttccatga aaaaaatttt
     4861 ataatagaaa ccatcaataa aaatgaataa aaaatcattg ataggattaa ctttagaaga
     4921 acttaaaact atcgtaaccg attttggaat gccttcttat acggctaaac aaattgcaga
     4981 atggttatat aaaaaacggg ttctttctat caatgaaatg accaatattt cttcaaaaaa
     5041 cagagccttg ctcaatgaga aatataaggt tggtcgttct gaacctcttc aagtaactat
     5101 ttcgaaagat ggaacaaaaa aatatttatt tgctactgaa aataaccatt ttatcgaaac
     5161 agttttgata cctgaagaaa atcgtgttac tctgtgcgtt tcttcacagg caggctgtaa
     5221 aatgaattgt ctgttttgta tgacaggaaa acaaggattt gtttctaaat tgtcagcagg
     5281 agaaattctt aatcaaattt tatccgttcc tgaagccagt gaaatcacca atttggtttt
     5341 tatgggcatg ggagaacctt ttgataacac aatagcagta ttaaccgctt tggaaattct
     5401 tacagccgat tatggatatg catggagtcc tcacagaata actgtatcga ctatcggact
     5461 tataccaggc atgaaacttt ttctggaaaa aaccaattgt catcttgcca ttagtttaca
     5521 ttcacccttt catgaagaaa ggcttcaatt aattcctgct gagaaagctt atcccatcaa
     5581 aaaaatatta gaggaattga aaaaatatga tttcagtaaa cagcgaagac tttcttttga
     5641 atatattatg tttgatcatt tcaacgacac tatgcgacat gctcgagaat tagtaaaatt
     5701 attgaaagaa attccttgcc gagtaaattt aattcgtttt catgctattc ctgatgtccc
     5761 attaaaaact tcgagtgaag aaaaaatact ttcgtttcgc aattacctga atgaaaatgg
     5821 aattattgcc actattcgta aatcgcgagg tgaagatatt tttgccgctt gcggcatgtt
     5881 gtccacatca gctaaagaaa aagaaactaa aaatcaattg taaaactgta tgaaaaaatc
     5941 aataaatatt ttttttaaga aaaattggct gatatttttt ttgttgtttt ttggagtaaa
     6001 tatctgcaat attcaagccc aacaattttt atctacaatt tcagattctc taacggcaat
     6061 tgctaatgaa gaaacatata taaaaagaat tgctataaaa gatttttcag taacccagaa
     6121 accaaaaaaa gtaattatta ctgctgatga aagattaagt tatatccctt tcaggcctga
     6181 aaatgtagaa agaatttaca acgctcttga aaaaattttg atgcctactt acgaaggtta
     6241 taaaattgtt tgtcaagtgg ataataaacc tattgaagaa ttaataccca atttttacag
     6301 aacggggaaa aaagataaaa gtagaatttt tataacaaaa aaaatagaat ttcctctggt
     6361 taaaaatctt tctcgtcctt atgaaattac tcggggtctt caaaacaaac acattgctgt
     6421 atggcccagt catggttatt actataatca aaatttagca cgatggcaat ggcaacgagg
     6481 acgacttttc caaacagtgg aagatattta tactctttcg tatatttacc cttatctcgt
     6541 accgatgctt gaaaatgctg gtgctaatgt tttaattccc agagaaagag atgtacaaaa
     6601 aaatgaaatt attgtggata atgatatgag cgatgactta tcaaaatact cagaaattaa
     6661 tcaatctttt cagtggaaaa acggacaaga tacaggtttt gcaaatcaaa aaaataatta
     6721 tctggatgga gaaaatccat ttatgatggg tacttaccgg caaattttat ctgtaaataa
     6781 caaagataaa gttagctctg ttgaatggat accttttatt cctcagtcag gtaaatatgc
     6841 cgtatatgtt tcttataaaa cgctcaccaa aagcacttct acagcacact acactgtata
     6901 tcataaaggt ggtcaaacag aatttttggt taatcaaaaa atgggaggcg gtacatggat
     6961 ttatttagga acattttatt ttaacgaagg ttgcaatgaa gacagtaaag tagttcttac
     7021 caatttaagt tcaaaaaaga atgaaatcat aactgctgac gctgtaaaat ttggtggtgg
     7081 cataggaaat attgcacgtc agcctttggc agaaggatta ataacaaatt tgaaaagttc
     7141 agaagaacta ataattgaaa aaaacaaaat ttcagacaaa aattttcatc aagatacaat
     7201 tcatgaacct gaaataagtg gttatccacg atttactgaa ggttcacgtt attggctgca
     7261 atgggctgga tttcctgata gtgtttacag ttttacaaaa aggaaaaatg attatattga
     7321 cgattatcaa tcacgcggat tgtgggtcaa ctatttgatt ggtggttcac cacttgctcc
     7381 atctgataag ggattaggaa ttccaattga tttatcgctg gcttttcata ctgatgctgg
     7441 aacaacaagc aatgattcta tcataggcac tttaggaatt tacactgtcc ttaattcgta
     7501 tcaaaaagat atttttaaaa acaaaatttc acgctggact tcacgcgatt taacagatat
     7561 cattcaaacg caaattgtga ctgatattcg taatatgtat gctccagagt ggactcgacg
     7621 taggatttgg aataaaaatt acagtgaatc aagattacca gaagtccctt ctatgttatt
     7681 ggaattactt tctcatcaaa attttgcaga tatgcgttat ggactcgacc cacgttttca
     7741 atttttagtg agccgctcta tttataaagg tatattaaaa tatctacatt ctgataatcc
     7801 caataaatat tgtgtacaac ccttgcctgt gaagcaattc agtgttcaat tcattgacac
     7861 aaccacttta aaattgcgat ggactaatga aatcgatttt ttggaaccaa ctgctgtacc
     7921 ggaatatttt gtagtgtata tacgaagaga tgaaaatgat tttgataatg ggattttagt
     7981 aaaaaataac gagtatactg tcaatattga accaggtaaa atttatagtt ttaaagtaac
     8041 agccgtgaat aaaggtggcg aaagttttcc ttctgaaatt ctttctgctt atcgggctcc
     8101 tgatgaaaaa ggtgaagtgt taattgtaaa tggctttacc cgattaagtg cacctgaaag
     8161 cttttcaata gatacgcttt atgcaggttt tgtaaatgac aaggatgcag gtgttcctta
     8221 tttgtatcaa tataatttta cagggaaaca atatgaattc agtaaaaata aactttgggt
     8281 taatgacgat gctcctggtt ttggcgccag ttatgcaaat tatgaaggaa aaatattagt
     8341 ggggaataca tttgattatc cttttttaca tggaaaagca attaaggaag caggttattc
     8401 attttcttct tgcagtagtg atgctattat caatgaagaa attaatctga atgattataa
     8461 aatggtagat ctaatcttgg gaaaacaaaa gcaaacctat ataggtaatg gtaaaaaatc
     8521 tactgctttt aaaactttcc cacttgattt gcaaagaaaa atcaatttat tttgccaaaa
     8581 tggaggaaat ttatttgtaa gtggagctaa tattgtttcc gatttatgtg ataatccatt
     8641 aactttgcct gaagaacgtt cttttcttga aaatacttta aaattaaaat attgcactca
     8701 tcaagctgct gtaacaggag aagtgaaatt tataaattca cctcttcctt tttttaaaaa
     8761 aattaatttt agctattttc acgagccaaa tgaattttgt tattttgttg aatcgccaga
     8821 tgcgtttgaa cctaatgata agaaagctta tacgatttgc agatattcag aaaacaattt
     8881 gagtgctgca gtagcttatg atggaaatta taaagtttgt gcctttggct tcccattcga
     8941 aactattatt tctgaaaacg atcgacaaaa tattatggaa tcaattttat catttttcac
     9001 aaaacattaa tcgtaattaa caaacaaaaa ttatcaattg aaaccttagc attctaaaaa
     9061 accttaatga cttataataa acaaatctgg caattttttt taaaatagaa tataaataca
     9121 aattaaaaag aaaaataagc cgccataaat aagttgaagt aaataatcaa cctaactaaa
     9181 tgaagccgac ttcaaataga taaacaaata aaatacatat acttttaagt agaaagacaa
     9241 tttttttaac ttcttatctg tttttttaaa atactgatat ggtttaactt aataaaatta
     9301 tttagagatg aaaaataaaa ttttgatatt actatcggct attttcttct tgttgggctg
     9361 ccagaaaaca aaaacttctt tacccgtagc cacaggtacc atgtttgaag ttatagtagt
     9421 gatggatgaa ccagcatggt cttctttacc aggaaacact ttacgtgaaa cattagatca
     9481 gccaatggaa gctttgcctc aacctgaacc cattattgat atgcatcatt gccaacatca
     9541 ggaattctca aacatgtata aatcatctcg gaatatttta attaccgaga tttctgaaaa
     9601 acatgtacaa ccaaaagtaa tctatacaaa agataaatgg gcttctcctc aagctattgt
     9661 tcagattact gctccaaatg acagttcttt tgcaaataca gttcgtcaat atggtaataa
     9721 aattttagat tactatgtta ttacagaacg tgaacgtttt attgaattta ataaaaatca
     9781 tttgaatgtg aaagcaaaaa cagaaataga acaaatgttt ggcacccaga ttgatgttcc
     9841 tagttttctg accaaagcta ccaagaaaaa agatttttat tggcttacca atgataatcc
     9901 taaaattcgt caagatttgg tgatttattc ttatccatat accgataaaa atacttttac
     9961 caaagaatat cttttagcaa aacgtgatac tgtaatgaaa accaatatac caggagaaat
    10021 tcagggttct tatatgggga cagaatacaa aaattatcca ccgattttta gggagatttg
    10081 ggttaatgga ggttattgtg ctgaaattaa aggactttgg aaaatgataa atggtgcagc
    10141 tatgggtggt cctttttaca gccatactcg cttagatgaa attaatcaaa aagtaataac
    10201 agtagaagga ttcgtatttg cgccaggaga aaagaaacga aatgctatcc gacaattaga
    10261 agcagttatt tatactacca aacttccaca ggaaattaat gcattaaaag aagtttctgt
    10321 tacaccaaaa aataattaga aaaatataac gaaacaataa tggataaaat aattattgga
    10381 attacacaag gagatattaa tggaatcggt tatgaagtga ttttaaaatc tttatctgat
    10441 gctgctattt ttgatttatt cacccctgtc atttacggct cttcaaaaat agcagcatat
    10501 catagaaaag ttttaaatat tcaaaacttg agttttaata atatcaattc agtagaagaa
    10561 gcaaatccaa aacgaattaa tattataaat tgtacgtccg atgaagtcaa agtagagtta
    10621 ggtcaaccta cggctgagag tggaaaagct gcacgtgatg ctttggaacg agcttgttct
    10681 gatctgggaa aaggtttaat ccatgctttg gtaacagctc ctatcaataa gaagaatatt
    10741 cagtcggaaa aatttcattt tccaggtcat actgaatatc tggctgaaaa atttggaaca
    10801 aacaaagcac ctcttatgat gctgatcaat gattttatgc gaatagctgt cgtaacagga
    10861 catattccct tgcagaaagt tgcctcaaca ttgacaacag aattgattct tgaaaagata
    10921 gaaacgctca atgattcgtt gaaggaagat tttttaattg ttcgtccacg tatcgccgta
    10981 ctgggactca atcctcacgc aggcgatgaa ggcgtaattg gtgacgaaga agaacaagta
    11041 attattccag cattgaaagc agccagcgaa aaaggaatta tttgtttagg accttatcct
    11101 gctgatggtt tttttggttc aggaaatttt tcaaattttg atggcgtttt agcaatgtat
    11161 cacgatcaag ggcttattcc atttaaaact ttatcagtag aaagtggtgt taattttacg
    11221 gctggattat caattgttcg aacttcacca gctcacggaa cagcttatga catagctgga
    11281 aaaaatttag cttcggaaaa ttctttcaga gaagctttgt acatggcttg tgatatctat
    11341 aaaaacagaa catggaataa tgaaataaaa tcaaatcctt taaaaacaat tccaaaatct
    11401 gaaaatgaaa caaacattca ggttgaataa acatgttgat gaataaaact caacaagaag
    11461 agctttttta tggctgtcaa tcaaccattt ttggagattg ctgccttgtt tttactagac
    11521 aaaacatttt gcgacttttc ttcaccgacg agagagaaaa aacacttcaa tattttgagc
    11581 aattaactga aaaaatgaat ttaaaaaggt atgatgaaaa agcaaaagaa ctaattgatc
    11641 aaatttttat agaaagaaaa acttttccat tagtattaaa aggaacagct ttccaattgt
    11701 ctgtctggaa agctttacaa accatacctt ttggcgaaac aaccacttac aaagttattg
    11761 ctgaggcaat tggtcgtcct aatgcaatac gagctgtagg aaatgctgtg ggagcaaatt
    11821 ctatagcttt tttaattccc tgtcatcgag ttattcggag tgatggacag ttaggcggtt
    11881 accgatgggg cgtggaacga aaacaaaaaa tgctggaatg ggaaaaaatc aacttcaaaa
    11941 ataaattcat ttattcagcc atcttttcgt taaatcctga taactatcaa tgcgtctgtc
    12001 gcgaaaaaat ggccagattc ggcgaacttg ttcacttctt gccaaatcaa tatttataag
    12061 caaattttct tccttctgat tggatgcttg agctaaaatt tctccctgcg gaccagccac
    12121 aaaactattt ccccaaaatt ggatgccagc cgtttgtccg gaaggatcat cttcacgacc
    12181 aactcggtta cacgaaacga caggcaaacc gttagcaatg gcatgacttc gctgaatggt
    12241 gatccacgct tcaagttgtc gctgcttttc ttcttctgaa tcagttgttt cccagccaat
    12301 agccgtagga taaatcagca attcagctcc ggccaatgcc atcaatcggg ctgcttcggg
    12361 aaaccattga tcccaacaaa ccatgactcc caattttccc aaagaagtct gaatgggtac
    12421 aaaacccaaa tcgccgggcg tgaaataaaa cttttcgtaa taagccggat catccggtat
    12481 gtgcattttt cgataaattc cggctattgt gccatccttt tcgagcaccg ctgccgtgtt
    12541 gtgatacaat cctgctgctc gcttttcgaa taacgataaa accaaaacta cacccaattc
    12601 tctggcaagt ttcccaaaat aatcggttga aggtccggga attgtttcgg caaggccaaa
    12661 ataatcggga ttttcggtct gacagaaata aagactattg tgcaattcct gcaaaacgac
    12721 caattccgca ccttcggcgg cacactttcg gatattttcc tgcaatttgt caagattggc
    12781 agatgtatct tttccgttcg attgctgaat caatgcaact ttcattttta taatattttt
    12841 ttgtgggtta tttgtgattg aatttcaact taaagtcgct ggaaataaat aatttgtttg
    12901 ctaaaaaaca aaaattattt ttcagacgtt gcatgcaacg tctctacaaa actataaatt
    12961 attgactatc aactatatgc taaaaatcca aactcttttc tcgaaacttg tatctaccct
    13021 ttcgccggat aaaactgcat cgttacgcaa tgcagcgaac cgtgctgttt gatcaacgtt
    13081 ctgcaatcga taccaataa
//
LOCUS       DAOSAS010000036        13030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3233_length_13030_cov_12.047322, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000036 DAOSAS010000000
VERSION     DAOSAS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13030
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3233_length_13030_cov_12.047322"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLR72_02795"
     CDS             <1..638
                     /locus_tag="PLR72_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOK36170.1"
                     /translation="EAKIEELMDAVDTWIQLPTRDIDKPFLMPVEDVFSITGRGTVAT
                     GRIETGIVKVGDEVQIIGLGQEGKKTVVTGVEMFRKILDQGQAGDNVGLLLRGIEKDE
                     VKRGMVITHPGAITPHKKFKAAVYILKKEEGGRHTPFHNHYRPQFYIRTMDVTGEITL
                     PEGVEMVMPGDNVEITVELIYPVAINVGLRFAIREGGRTVGSGQITEILDE"
     gene            752..825
                     /locus_tag="PLR72_02800"
     tRNA            752..825
                     /locus_tag="PLR72_02800"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:786..788,aa:Trp,seq:cca)
     gene            841..1035
                     /gene="secE"
                     /locus_tag="PLR72_02805"
     CDS             841..1035
                     /gene="secE"
                     /locus_tag="PLR72_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOK36171.1"
                     /translation="MKKIINYIKESYSELVHKVSWPTLSEVTNTAVVVLIASIILALI
                     VWIMDVSFENIMTFIYEKLL"
     gene            1060..1608
                     /gene="nusG"
                     /locus_tag="PLR72_02810"
     CDS             1060..1608
                     /gene="nusG"
                     /locus_tag="PLR72_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOK36172.1"
                     /translation="MTDSANLKWYVLRAISGKENKVKEYLESEMKNSDLGQYVHQVLI
                     PTEKVYQIRNGKKIAKERNYMPGYVLIEANLFGEVIHRVRNIPNVLGFLGEKNKIPTP
                     IRPSELNRILGTMDELQESEEEILTPFYKGDNVKVTYGPFSGFIGTIDEVNTEKKKLK
                     VMVTIFGRKTPLELSFTQVEKE"
     gene            1658..2101
                     /gene="rplK"
                     /locus_tag="PLR72_02815"
     CDS             1658..2101
                     /gene="rplK"
                     /locus_tag="PLR72_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOK36173.1"
                     /translation="MAKEIAGIIKLQIKGGAANPSPPVGPALGSKGINIMEFCKQFNA
                     RTQDKAGKILPVIITYYTDKSFDFVVKTPPVAIQLLEITKLKSGSAEPNRKKVAEITW
                     EQVRNIAKDKMVDMNCFEEEAAMKMVAGTARSMGIVVKGDFPGNN"
     gene            2119..2814
                     /gene="rplA"
                     /locus_tag="PLR72_02820"
     CDS             2119..2814
                     /gene="rplA"
                     /locus_tag="PLR72_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOK36174.1"
                     /translation="MTTLTKNRKIALEKIEPGKAYPLKEAAKLVKEITTTKFDASVDI
                     DVRLGVDPRKSNQMVRGVVTLPHGTGKKVTVLALCPPDMEAAAKEAGADYVGLDEYIE
                     KIKNGWTDVDVIITTPSVMGKLGALGRILGPRGLMPNPKSGTVTNDIAKAIKEVKQGK
                     IDFKVDKYGIVHASIGKVSFTPEQIAENAREFITTLIKLKPATAKGTYIKSIYLSSTM
                     SPGIKVDPKSIED"
     gene            2828..3343
                     /gene="rplJ"
                     /locus_tag="PLR72_02825"
     CDS             2828..3343
                     /gene="rplJ"
                     /locus_tag="PLR72_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOK36175.1"
                     /translation="MKKEEKGKIIQEIESILSKYPHFYLTDIGGLNGAQTSELRRICY
                     KEKIKLMVVKNTLLKKALDNSSTNFSDLYPILKGETALMLSEVANLPAKVIKDFSTKN
                     KKPVLKAAYVEESFYIGENQLETLIHIKSKNELIGEIINLLQSPLSNVLSALQSGGTT
                     IHGLLKTLSEK"
     gene            3385..3762
                     /gene="rplL"
                     /locus_tag="PLR72_02830"
     CDS             3385..3762
                     /gene="rplL"
                     /locus_tag="PLR72_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOK36176.1"
                     /translation="MTDLKAFAEQLVNLTVKEVNELAQILKEEYGIEPAAATVAVSNA
                     PAAAAAEEKPEKTSFDVVLKSPGPNKLAVVKLVKDLTGLGLKDAKDLVDSAPSVVKEG
                     LSKEEAETLQKQLSDGGAEVELK"
     gene            3879..7691
                     /gene="rpoB"
                     /locus_tag="PLR72_02835"
     CDS             3879..7691
                     /gene="rpoB"
                     /locus_tag="PLR72_02835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOK36177.1"
                     /translation="MSSLTNTERINFSTIKNQLEYPDFLDVQLKSFQDFFQMDTPPEK
                     RKLEGLYKVFTENFPVSDTRNNFVLEFLDYFIDPPRYSIEECIERGLTYNVNLKAKLK
                     LYCTDPEHEDFDTVIQDVFLGPIPYMTPRGTFVINGAERVIVSQLHRSPGVFFGESLH
                     ANGTKLFSARIIPFRGSWIEFATDINNIMYAYIDRKKKLPVTTLLRAIGFESDKDILE
                     IFDLAEEVKVSRSNLKKYIGRKLAARVLKSWLEDFVDEDTGEVVTIERNEIIIDRETV
                     LENEHIDLILEAGAQTILLHKEEADQIDYSIIYNTLQKDPTNSEKEAVLYIYRQLRNA
                     EPPDDATAREVINNLFFSEKRYDLGDVGRFRINQKLNLNISNETKVLTKEDIIEIIKY
                     LVLLINSKSTVDDIDHLSNRRVRTIGEQLYNQFNVALARMVRTIRERMNVRDNEVFTP
                     VDLINVKTISSVINSFFGTNALSQFMDQQNPLAEITHKRRMSALGPGGLSRERAGFEV
                     RDVHYTHYGRLCPIETPEGPNIGLISSLCVYAKINELGFIETPYRKVNNGIVDLSENG
                     VSYYTAEEEEDLVVAQGNAPLNEDGKFIRNKVKARLGADFPIVDPSEVDLMDVSPSQI
                     ASIAAALIPFLEHDDANRALMGSNMMRQAVPLLRCEAPVVATGIEGRVIRDSRTQILA
                     EGEGVVEYVDATSIKIRYDRTDEEELVSFDSNIKEYKLSKFLKTNQNTTIDLRPLVRK
                     GDKVHAGQIITEGYCSEKGELALGRNLKVAFMPWKGYNFEDAIVISERVVREDIFTSV
                     HVVEQLLEVRETKRGPEEFTADIPNVSEEATKNLDENGIIRIGAWVEPGDIIIGKITP
                     KGESDPSPEEKLLRAIFGDKAGDVKDASLKATPSLSGVVIDKRLFSKALKNKKSKLAE
                     KAILPKVEEEFEAQTKALKEALIEKLMIILNNKTSAGVKDFLGADVISRNSKFTPERL
                     QEIDYATVQLTKWTNDAHTNELVSQVILNYLKKYKELDAQLKRQKFNIAIGDELPSGI
                     MQMAKVYIAKKRKVQVGDKLAGRHGNKGIISRIVREEDMPFLEDGTPVDIVLNPLGVP
                     SRMNLGQIFETILGWAGKELGVKFATPIFDGATMDDLNKWTDKAGLPRGGKTYLYDGG
                     TGERFDQPATVGVIYMMKLGHMVEDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEME
                     VWALEAFGAAHTLREILTVKSDDVNGRARIYEAIVKGDPMPTPGIPESLNVLLHELRG
                     LCLSINLE"
     gene            7758..12059
                     /gene="rpoC"
                     /locus_tag="PLR72_02840"
     CDS             7758..12059
                     /gene="rpoC"
                     /locus_tag="PLR72_02840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOK36178.1"
                     /translation="MAFKTDNKIKSNFTKISIGLASPEEILKLSHGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIQ
                     LVVPVAHIWYFRSIPNKIGYLLGMPTKKLDAIIYYEKYVIIQGGILENGENITQGELL
                     SEEEYLDILDTLPRDNQLLDDSDPNKFIAKMGAEALYDLLSRLDLDALSYELRNKANT
                     DTSQQRKTEALKRLQVVEAFRASRNRNKPEWMILKVIPVIPPELRPLVPLDGGRFATS
                     DLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLLDNSRKVSAVKTESN
                     RPLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKNMAAELYKP
                     FIIRKLIERGIVKTVKSAKKIIDRKDPVIWDILEYVMKGHPVLLNRAPTLHRLGIQAF
                     QPKLIEGLAIQLHPLSCTAFNADFDGDQMAVHLPLSNEAILEAQLLMLSSHNILNPAN
                     GAPITVPSQDMVLGLYYITKPRKGVKGEGLIFYSPEEAEIAYNEGKVDLHAWIKVRIS
                     ETNQEGKNVKKIIETTVGRILFNKCVPPEVGYINELLSKKSLRDIISKVIETCGFART
                     SQFLDDIKDLGYYMAFKGGLSFNMDDVIIPKEKEQLIQEGNAEIEEIMNNYNMGFITY
                     NERYNQIIDTWTHVNSKLSDVLIKKLSSDQDGFNSVFMMLDSGARGSKEQIRQLSGMR
                     GLMAKPQKSGAEGGQIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYL
                     TRRLVDVAQDVTITQEDCGTLRGLVANELKNNEEVITSLYERILGRVSVHNIYHPQTG
                     ELIVAAGQEITEKEAQIIQNSPIEHVEIRSVLTCEAKKGVCAKCYGRNLATGKMIQIG
                     EAVGVIAAQSIGEPGTQLTLRTFHVGGIAGNIAAESKLVLRYDGRLEFDELRTVQPTD
                     NVNYQVVISRLAEMRVIDPKTGIVLSTHNIPYGSKLYVKNGDLGTKGQLICEWDPFNA
                     VIISENEGIIKFEDLVENVTFKMETDEATGLREKIIIESKDRNKIPTAYIVDENGNIL
                     RTYNLPVGAHIVFENGDKIKAGQILVKIPRAIGKTGDITGGLPRVTELFEARNPSNPA
                     VVAEIDGEVSLGKIKRGNREIIITSKSGEVKKYLIPLSKQILVQENDFVRAGTPLSDG
                     AITPTDILQIKGPTAVQEYIMNEIQDVYRLQGVKINDKHFEIIVRQMMRKVEIVEAGD
                     TRFLEKQVVDKNEFMEENDRIWGKKVVVDAGDSPNLKPGQIITPRKLRDENSILKRKD
                     LRLVQARDAIPATSNQILQGITRAALQTTSFMSAASFQETTKVLTEAAINGKVDKLEG
                     LKENVICGHLIPAGTGQRDFEKIIVYSREEYEKKTFARKNVLDMENEELVFEEEE"
BASE COUNT         4550 a         1916 c         2600 g         3964 t
ORIGIN      
        1 gggaagccaa aattgaagag ttaatggatg ccgttgatac ttggattcaa ttaccaacaa
       61 gggatattga taagcctttc ttaatgccag tagaagatgt cttttcaatt acaggtcgag
      121 gaacggtagc aacaggacgt atcgaaacag gaatagtaaa agtgggtgat gaagtacaaa
      181 ttatcggttt agggcaggaa ggtaaaaaaa cagtagtaac cggtgtggaa atgttccgta
      241 aaatcttaga tcaaggacaa gcaggcgata atgtaggatt attgcttcgt ggcattgaaa
      301 aagatgaagt aaaacgtggt atggtaatca ctcatccggg tgcaattaca cctcataaaa
      361 aattcaaagc tgctgtttat attttgaaaa aagaagaagg tggacgtcat actccattcc
      421 acaatcacta tcgtcctcaa ttctatattc gtactatgga tgttacgggc gaaattaccc
      481 ttcctgaagg agttgaaatg gtaatgcctg gagataatgt tgagatcact gtcgaattaa
      541 tatatccagt tgctattaat gtgggattac gttttgctat tcgtgaaggt ggtcgtacag
      601 tgggctctgg acaaattact gaaattcttg atgaataaaa taaattggta ttttatcaat
      661 tcccacccta taaatggaat ttcaaaaaat aaaattatta aaacctcttt ttcaagcttt
      721 aaagcttgaa aggaggattt taatgttgta gacgggcgta gctcagtttg gtagagcact
      781 ggtctccaaa accaggtgtc gggagttcga gtctctctgc ccgtgcaaat ctatgataga
      841 atgaaaaaaa taattaatta tatcaaagag tcttattcag aactggttca taaagtatcg
      901 tggccaactt tatcagaagt tactaacact gcagttgtag tattgattgc ttccatcata
      961 cttgcactga tcgtatggat tatggacgtt agttttgaaa atatcatgac attcatttat
     1021 gaaaaattgt tataatttaa ttagcgggag gtcaatactg tgactgattc agcaaattta
     1081 aagtggtatg ttttacgtgc cattagtggg aaagaaaata aggtaaagga atatcttgaa
     1141 tcagagatga aaaattctga tttaggtcag tatgttcatc aagtattgat tccaactgaa
     1201 aaagtttatc aaattcgtaa cggtaaaaaa attgctaaag aacgcaatta tatgccagga
     1261 tatgttttga tagaagctaa tctttttggt gaggtaattc atcgtgtcag aaatataccc
     1321 aacgtgttgg gatttcttgg tgagaaaaat aaaataccta cccccattcg tccttcagaa
     1381 ttaaatagaa ttttaggtac aatggatgaa cttcaagaaa gtgaagaaga aatccttaca
     1441 cctttttata aaggagataa tgtaaaagta acttatggac ctttttctgg atttataggg
     1501 acaattgatg aggtaaacac tgaaaagaaa aaactcaaag ttatggttac tatattcggt
     1561 cggaaaactc cgctcgaatt aagttttact caagttgaaa aggaatagta gtttttaatt
     1621 tttactattc cattaaaaaa tcaaaattta aacaattatg gcaaaagaaa ttgctggaat
     1681 tattaaatta caaatcaaag gaggtgcagc aaacccctcc ccgcctgttg gtcctgcttt
     1741 aggctcaaaa ggtatcaata tcatggagtt ttgcaaacag tttaatgcac gaactcaaga
     1801 caaagcaggt aaaatcttac ccgttatcat tacgtattat actgataaat cgtttgattt
     1861 tgtagtaaaa acaccacctg ttgcaataca attacttgaa attaccaaac ttaaaagtgg
     1921 ctcagcagaa cccaatcgta agaaagttgc tgaaatcacg tgggagcaag tgagaaacat
     1981 tgcaaaagat aagatggtgg acatgaattg ttttgaggaa gaagctgcaa tgaaaatggt
     2041 tgctggtaca gcaagaagca tggggattgt tgtaaaaggt gattttccag gtaataatta
     2101 actaacactt taattacaat gactacttta acaaaaaata gaaaaattgc tttagaaaag
     2161 attgaaccag ggaaagccta tcctctgaaa gaagcagcta aattagtgaa agaaattact
     2221 accactaaat ttgatgcttc agttgacatt gatgttcgat tgggagttga cccacgtaaa
     2281 tctaatcaga tggttcgtgg tgtagttact ttgcctcatg gaacagggaa aaaagttact
     2341 gtacttgctt tatgtcctcc tgatatggaa gctgcagcta aagaagcagg agccgattat
     2401 gtagggttgg atgaatatat tgagaaaatt aaaaatggat ggacagatgt agatgtaatt
     2461 atcacaacgc cttccgttat gggtaaatta ggagctttgg gacggatttt gggaccacgc
     2521 ggattaatgc ctaatcctaa aagtggtact gtaaccaatg atattgccaa ggcaataaaa
     2581 gaagttaaac aaggtaaaat tgattttaaa gtggataaat atggtattgt tcatgcttcg
     2641 atcggtaaag tttcttttac gcccgaacag attgctgaaa atgccagaga gtttattaca
     2701 actttaatca aattaaaacc tgctactgct aaaggtactt acataaaaag tatttatctt
     2761 tccagtacta tgagcccagg catcaaagta gatccaaaat ctatagaaga ttaaaaaata
     2821 ttcatttatg aaaaaagaag agaaaggaaa aataattcaa gaaattgagt caattttaag
     2881 caagtatcct catttttatc tcacagatat tggtggctta aatggcgcac aaaccagtga
     2941 attaaggaga atatgctata aggaaaaaat taagctgatg gttgtaaaaa acacattgct
     3001 taaaaaagct ctcgataatt cttcaactaa ttttagtgat ctttatccca ttctaaaagg
     3061 agaaacagct ttgatgcttt cagaggtagc aaatttgcct gctaaagtta ttaaagattt
     3121 tagtaccaaa aataaaaaac cggtacttaa agctgcttat gttgaagaaa gtttttatat
     3181 tggtgaaaat cagttggaaa cattgattca tattaagagc aagaatgagc ttattggcga
     3241 aataattaat ttgttacagt caccattaag caatgtattg tccgcactcc aatcaggagg
     3301 tactactatt catggcttgt taaaaacatt gtcagaaaaa taatttagta aaaacaaaaa
     3361 taaaaattaa aaatcatagt aaaaatgaca gatttgaaag cttttgcaga acaattagtt
     3421 aacttaacag ttaaagaagt aaacgagtta gctcaaattt taaaagaaga atatggcatc
     3481 gaacccgcag cagcaacagt agctgtaagc aatgctcctg ctgcagcagc tgctgaagaa
     3541 aaacctgaaa aaacatcttt tgatgtagta ttaaaatctc ctggtcccaa taaacttgca
     3601 gtagttaaat tagtaaaaga tcttacagga cttggattga aagatgctaa agacttggtt
     3661 gactcagctc cttcagtagt taaagaaggt ctttcaaaag aagaagctga aaccttgcaa
     3721 aaacaactta gtgacggcgg agctgaagtt gaacttaaat aatattaact gctgattatt
     3781 aacttattgg tttagactcc ctataaaaag ggggttctaa accttttcct atctaacatg
     3841 ataaaagatc tttggaacaa aaataacctc tttgaaaaat gtcttcatta actaacactg
     3901 aaagaattaa tttttccaca ataaaaaatc agcttgagta tcctgatttc ttagacgtac
     3961 aattaaaatc tttccaggat ttcttccaga tggatactcc tcctgaaaaa agaaaactag
     4021 aaggattata taaagtattt actgaaaact ttcctgtttc tgatacacgt aataattttg
     4081 ttcttgaatt tttagattat tttatagatc caccacgtta ttccatagaa gaatgtattg
     4141 aacgaggttt aacttataat gtcaatttaa aagcaaaatt aaaactttat tgtacggatc
     4201 cggaacatga agattttgat actgttattc aagatgtatt tttaggccct attccctata
     4261 tgactccaag aggcactttc gtaattaatg gtgctgaaag agtaatagtt tctcaacttc
     4321 atcggtcacc tggtgtattc tttggagaga gcctccatgc taatggaact aaattatttt
     4381 cagcacgtat tattcctttc cgtggctcat ggatagaatt tgcaactgat attaataata
     4441 tcatgtatgc ttatattgac agaaagaaaa aacttcctgt tactaccttg cttcgtgcta
     4501 ttggtttcga aagcgataaa gatattctgg aaatttttga tcttgctgaa gaagtaaaag
     4561 tatccagatc gaatctaaaa aaatatatag ggcgtaaatt ggctgcccgt gttttaaaaa
     4621 gctggctcga ggattttgta gatgaggaca cgggtgaagt tgttaccatt gagcgaaacg
     4681 aaatcattat tgatcgtgaa acagtgctgg agaatgagca cattgattta atactcgaag
     4741 cgggtgctca aacaattctt ttacataaag aagaagcaga tcagattgac tatagtatta
     4801 tttataatac tttgcaaaaa gatccgacca attcggaaaa ggaagctgtg ctctatattt
     4861 acaggcaatt acgaaatgca gaaccacctg atgatgctac agctcgtgaa gtgatcaata
     4921 atttattctt ttctgaaaaa aggtacgact tgggagatgt aggacgattt agaatcaacc
     4981 aaaaattaaa cttgaatatt agtaatgaaa caaaggtcct gaccaaagaa gatattattg
     5041 aaattattaa gtatctcgtt ttactgatta attcaaaatc tacggttgat gatattgacc
     5101 atttgagtaa tcgaagagtt cgcactattg gagagcagtt atacaatcaa tttaatgtag
     5161 ccttagcccg tatggtacgt accatacgtg agcgaatgaa tgtacgagac aacgaagtat
     5221 ttactccagt agatttgatt aatgttaaaa caatctcttc ggttatcaac tctttttttg
     5281 gtaccaatgc attatcacaa tttatggatc aacaaaatcc attagcagaa attacccata
     5341 aacgtaggat gtcagctttg ggtcccggag gcttatcacg tgaacgtgca ggctttgaag
     5401 ttcgagatgt acattatact cattacgggc gtttatgtcc tattgaaaca cctgaaggac
     5461 ctaatattgg attgatttct tctctgtgtg tttatgcaaa aatcaatgaa ttaggtttta
     5521 ttgaaacgcc ctacagaaaa gtaaataatg gcatagttga tttatcagaa aatggagttt
     5581 cttattatac tgctgaagaa gaagaagatt tggtagttgc ccagggaaat gcaccactga
     5641 atgaagacgg gaaatttatt cgcaacaagg taaaagcacg tttaggagcc gattttccta
     5701 tagttgatcc ttctgaagta gatttaatgg atgtgtctcc ttcgcaaatt gcttctattg
     5761 cagcagcctt gattcctttt ctagaacatg atgatgccaa tcgggctttg atgggttcta
     5821 atatgatgcg tcaggctgtt cccttgttac gatgcgaagc tcctgttgta gcaacaggca
     5881 tcgaaggacg ggttatacgg gattcaagaa ctcaaatact ggctgaaggg gaaggtgtgg
     5941 tagaatatgt tgatgccact tctatcaaga ttcgttatga tcgaaccgac gaagaagagc
     6001 tcgtaagttt tgattcaaac ataaaagaat acaaattatc taaattttta aaaactaatc
     6061 aaaatactac cattgattta cgaccattgg ttcggaaagg tgataaagtt catgcaggtc
     6121 aaatcattac agaaggatat tgtagtgaaa aaggagaatt agcattaggc agaaatttga
     6181 aggtggcgtt tatgccctgg aaaggatata attttgaaga tgctattgtg attagcgaaa
     6241 gagtggtacg cgaagatatt tttacttcgg tacatgtggt agaacagtta ttagaggtaa
     6301 gagaaacaaa acgtggacca gaagagttta cagcagatat tccaaatgtt agtgaagaag
     6361 caacaaaaaa tttggatgaa aatggaatta tccgtattgg tgcatgggta gagccaggcg
     6421 atattattat tggaaagatt actccaaaag gagaatcaga tccatcacca gaagaaaaat
     6481 tacttcgtgc tatttttggc gataaagctg gcgatgtaaa agatgcttca ttaaaagcaa
     6541 ctccttcctt atctggtgtc gtaatagaca aacgtttatt ttcaaaagct ttgaaaaata
     6601 agaaatcaaa acttgctgaa aaggctattc tgcccaaagt ggaagaggaa tttgaagctc
     6661 aaacaaaagc attaaaagag gctttaattg aaaaattgat gattattcta aacaataaaa
     6721 cttcagcggg tgttaaagat tttttaggtg ctgatgttat ctccagaaat agtaaattta
     6781 cacctgagcg tctccaagaa atagattatg ctactgttca gttgacaaaa tggacaaacg
     6841 atgcccatac caatgaactg gtttcacagg ttattctcaa ttatttgaaa aaatataagg
     6901 aattagatgc ccagttaaag cgtcagaagt ttaatattgc aataggcgat gaactcccaa
     6961 gtggaattat gcaaatggca aaagtttata ttgctaaaaa acgaaaagtc caggtaggag
     7021 ataaacttgc tgggcgacat ggaaacaagg gaatcatttc ccgtattgta cgagaggaag
     7081 acatgccttt cttggaagat ggaacaccag tagatatagt tttaaatcct cttggggtgc
     7141 cttctcgtat gaatctcgga caaatttttg aaactatttt aggttgggct ggaaaagaat
     7201 taggagtaaa gtttgcaaca ccaatttttg atggagcaac aatggatgat ttgaataagt
     7261 ggactgataa agctggatta ccacgagggg gaaaaactta tctttacgat ggtggaacag
     7321 gtgaacgatt tgatcaaccc gctacagtag gcgttattta tatgatgaag ttaggtcaca
     7381 tggttgagga taagatgcat gcacgttcta tcggacctta ttcactcata actcaacaac
     7441 cgttaggcgg aaaagcacaa tttggtggac aacgatttgg agaaatggaa gtttgggcat
     7501 tagaagcatt tggtgctgct catacgcttc gagaaatttt aacggtgaaa tcggatgatg
     7561 tgaatggtag agcacgtata tatgaagcaa ttgtaaaggg agatccgatg cctacaccag
     7621 gcattccaga atctttaaat gtattgttgc acgagctacg tggtttgtgt ttaagcataa
     7681 atttggaata aattctcagt aacaaaaagc tgaggaaaag ttagtttcaa caattataaa
     7741 ttgtaatttt taaaaatatg gcttttaaaa ccgacaataa aataaaaagt aattttacta
     7801 aaatttctat tgggttagct tcacctgaag aaattttaaa attatctcat ggcgaggtat
     7861 taaaacccga gaccatcaat taccgtactt ataagcctga acgtgatggc ttattttgcg
     7921 aacgtatttt tggtcctgta aaggattacg aatgccattg tggtaaatac aagcgtatcc
     7981 gctataaagg tattgtgtgt gatcgttgtg gagtagaagt tactgaaaag aaagttcgcc
     8041 gtgaaagaat gggacacatt cagttagtgg ttcccgttgc acatatctgg tattttcgtt
     8101 caataccaaa taaaattggc tatttgttag gaatgcccac taaaaagctg gatgccatta
     8161 tttattatga aaaatatgtc attattcagg gtggaattct tgaaaatgga gaaaatatta
     8221 cacaaggaga attgctctcg gaggaagaat atttggatat tttagatact cttccacgcg
     8281 ataatcaact tttagatgat agtgacccca acaagtttat tgctaagatg ggagctgaag
     8341 ccctttatga tttattatca cgattagatt tagacgcttt atcttatgaa ttaagaaata
     8401 aagcgaatac agatacttct caacaacgta aaacagaagc tttaaaacgg ttacaagtag
     8461 tagaagcttt cagagcttca cggaacagaa ataaaccaga atggatgata ttgaaagtga
     8521 ttccagttat tcctcccgaa ctacgtcctt tagttcctct tgatggtgga cggtttgcta
     8581 cttcggattt gaatgactta tatcgacgtg tcattattcg gaataatcgt ttgaaaagac
     8641 taatcgagat taaagctcca gaagtaattt tacgtaatga aaaacgtatg ctccaggaag
     8701 cagttgattc attgctcgat aattctcgta aagttagtgc agttaaaaca gaatcgaaca
     8761 gacctttaaa gtctctttct gattcattaa aaggaaaaca aggacgattc cgtcaaaatc
     8821 tgttaggtaa gcgtgtggat tattcggctc gttcggtgat tgtagtaggt ccagaattga
     8881 aaatgcatga gtgcggtttg ccgaagaata tggctgctga actttataaa ccttttatta
     8941 ttcgtaaatt gattgaaaga ggaattgtaa agacagtaaa atcggcaaaa aaaatcattg
     9001 atagaaaaga tcctgtgatt tgggatattt tggaatatgt tatgaaaggg catcctgtac
     9061 ttttaaaccg tgctccaacc ttgcaccgct taggtattca ggcttttcaa ccaaaattaa
     9121 ttgagggact ggctattcaa cttcatccgt tatcgtgtac agctttcaat gctgactttg
     9181 atggtgacca gatggcagtt catttgcctt taagcaacga agccatactt gaagctcaat
     9241 tgttgatgtt atcttctcat aatattttaa atccagctaa cggagctcct attactgttc
     9301 catcacaaga tatggtattg ggactttatt acattactaa acctcgcaaa ggtgttaagg
     9361 gagaaggatt gattttttat tcgccagaag aagctgaaat tgcttacaat gaaggaaaag
     9421 ttgacttaca tgcatggatc aaggtaagaa tatcggagac taaccaagaa ggtaaaaatg
     9481 ttaaaaaaat tatagaaact acggttggaa gaattttatt taacaaatgt gtgccaccag
     9541 aagtaggtta catcaatgaa cttctttcaa aaaaatctct tcgagatatt atttcaaaag
     9601 taattgaaac atgtggtttt gctcgtactt ctcaattcct ggatgatatt aaagatttgg
     9661 gatattacat ggcatttaaa ggagggcttt cgtttaatat ggatgatgta attattccta
     9721 aagaaaaaga gcaacttatt caggaaggca atgcagaaat tgaggaaatc atgaataatt
     9781 acaacatggg attcattact tataatgaac gatacaatca gattattgat acctggaccc
     9841 atgtaaattc aaaattatca gatgttttaa taaaaaaact ttccagtgat caagacggat
     9901 ttaactctgt atttatgatg cttgattctg gtgctcgtgg ctcaaaagaa caaattcgac
     9961 aattatcagg aatgcgaggt ttgatggcaa aacctcaaaa atcaggagct gaaggagggc
    10021 aaatcattga aaatcctatc ttgtcaaatt tcaaagaagg tctttctgtg cttgaatact
    10081 ttatttctac tcatggtgca cggaaaggtt tagcagatac agctttaaaa acggcggatg
    10141 caggatattt aactcgacgc ttagtggatg ttgctcagga tgttactata acgcaagaag
    10201 attgcggaac tttacgtggt ttggtagcca atgagcttaa aaataatgag gaagttatta
    10261 cttctcttta tgaacggatt ttaggacgtg tttctgtaca taatatttat catcctcaaa
    10321 caggtgaact aattgttgct gctggtcaag aaattactga aaaagaggca caaattattc
    10381 agaattctcc gattgagcat gttgaaattc gttccgtatt aacttgcgaa gctaaaaaag
    10441 gtgtttgtgc aaaatgttat ggacgaaatc ttgcaacagg caaaatgatt caaattggag
    10501 aagctgttgg agtaattgct gcacaatcta ttggcgaacc aggtacacaa ttaacacttc
    10561 gtactttcca tgtgggtggt attgcgggaa acattgcagc cgaatcaaaa ttagtattaa
    10621 ggtatgatgg acgtttagaa tttgatgaac ttcgcactgt acaacctact gataatgtta
    10681 attatcaagt agttataagt cgtctggctg aaatgcgggt aattgatcct aaaactggta
    10741 ttgttctttc tactcataat attccttatg gttcaaaatt atatgtaaag aatggagatt
    10801 taggaacaaa aggacaattg atttgtgagt gggatccgtt taatgcagtc attatttctg
    10861 aaaatgaagg aattatcaaa ttcgaagatt tagttgagaa tgttaccttc aaaatggaaa
    10921 ctgatgaagc aacaggatta cgtgaaaaaa ttatcataga gtcaaaagac aggaataaaa
    10981 tacctactgc ttatattgtt gacgaaaatg ggaatatttt acgtacctat aatttacctg
    11041 ttggtgctca cattgtgttt gaaaatggag ataaaataaa agcaggacag attttagtga
    11101 aaattccacg tgccatagga aaaacgggtg atattactgg tggtttgccc cgagtaacgg
    11161 aattatttga agctcggaat ccatccaatc ctgctgtggt tgctgaaatt gatggcgaag
    11221 tatcgttagg taaaattaaa cgtggtaatc gtgaaataat tattacctca aaatcaggcg
    11281 aagtaaagaa gtatttgatt cctttgtcca agcaaattct tgtccaggaa aatgactttg
    11341 tacgagcagg tacacctttg tctgatggag caattactcc tacagatatt ctgcaaatta
    11401 aaggtcctac tgctgttcag gaatacatta tgaatgaaat tcaagatgtg tatcgacttc
    11461 aaggggtaaa aataaatgat aagcactttg aaattattgt tcgtcagatg atgcgtaaag
    11521 tggaaattgt tgaagcaggc gatactcgtt tccttgaaaa acaagtggtt gataagaatg
    11581 aatttatgga agaaaatgat cgtatttggg gaaaaaaagt ggtggtagat gctggtgatt
    11641 cgcccaatct gaaaccagga caaattataa cccctcgaaa attacgtgac gaaaacagta
    11701 tattaaaacg taaagatttg cggctggttc aagcacgtga tgctattcct gctacttcaa
    11761 atcagatttt acaaggaatt acgcgggctg ctcttcaaac aactagcttt atgtcggctg
    11821 cttctttcca ggaaacgaca aaagttttga cagaagctgc aatcaatgga aaagtagata
    11881 agctcgaagg attgaaagaa aatgtaatct gtggacattt aatacctgca ggtactgggc
    11941 aacgcgattt cgagaaaatt atagtttatt ctcgcgaaga atacgaaaag aaaacatttg
    12001 cccgtaaaaa tgtgctggat atggaaaatg aagaattggt attcgaagaa gaagaataag
    12061 tttatttttc aagtataatt ttaaacaaac gaggtggtta tcttgataaa gccacctcgt
    12121 ttgctgtctt tttgatttcg ttaatttcaa taataattac ataaaatgta aaaattgttt
    12181 gcatatatat agaaaatttt tcaatataga gaaaaatctt cttctaagca aaagattatg
    12241 ttcgttttaa aaaagtgttt aaatgcaaat taaaaggtat ttaatcaaaa gaattcctcg
    12301 cggcttgcct cagggttatt catttcaaaa agaacattaa caagtagaat ttcgtcatat
    12361 gaaaagataa attttttttt gtaatgaatg aatcgcttga ataaggtaat aaggttataa
    12421 tcaggaagaa attttattat tattaaggtt aaatgtttta taataaggtt ttaaagaaaa
    12481 ttaaagtagg atataatgag caaaaaatta attatgtagg agaattataa tttcacagag
    12541 agaattttta taatggtttt acacaagaaa ccacaaaagt tattgaaaaa agttgatttt
    12601 ttattgttga ataattaaag tgagttttta aatgataacc ttatcatgta aaaaaccttc
    12661 ataacaagaa ataaacaaac aaaccaggcc ttattacttt attgaattga ttttgaaagt
    12721 tgatggcaca aattttttat tgtttatcaa gaaaaaagaa aatatcaagt atttatgggc
    12781 aatattttct ttctaactaa taatttgtgt aattataaat tgaaatttat ataagttaaa
    12841 aattgcttgg caatgaaaat caaagaaaat aattggttgc ttactgctat tgtaataggt
    12901 tcttttttta atagttttat gtcctcttct ttaaatattg ccatgccagt tattgatgca
    12961 gaatatcatg ttggcttagc ggaagtggca tggatttcaa aatcgtttct tttggcttca
    13021 gcagtaggat
//
LOCUS       DAOSAS010000037        12973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3247_length_12973_cov_12.789441, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000037 DAOSAS010000000
VERSION     DAOSAS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12973
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3247_length_12973_cov_12.789441"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /gene="lon"
                     /locus_tag="PLR72_02845"
     CDS             complement(<1..1577)
                     /gene="lon"
                     /locus_tag="PLR72_02845"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOK36179.1"
                     /translation="MSIQEDHPSNNKLNLGETLYILPLRNMVLFPGVLLPVSVGRAKS
                     LRLVKKARENDLLIGVCAQKDKNIEDPTSEQLYKVGTVAKIVKIFEMNDNTTTVILEG
                     GPRFRIAEFQTEKPFFKAKIEIAEEKIPETDDTVFLALISNIKEVATKIISNSGVMPP
                     ENIFAIRNIDNPVFLINYICVNFGLNMEDKQRLLEIDDIKERGYQLLDLLNKELQLLE
                     IKISIQNKAKEGIDKQQREYFLQQQLKTIQDELGGTSPEEDIVEFRERAKQKKWNESV
                     AKVFNKELNKLERMSTYSPDYSIQLNYVEMMLDLPWNEYTKDNFNLKNARKILDKDHF
                     GLETVKERILEHLAVLKLKGDMKAPILCLYGPPGTGKTSLAKSIAVALNRKYVRVSLG
                     GLHDEAEIRGHRRTYIGALPGRIIQNIQKAQSANPVFVLDEIDKVSADYKGDPSSALL
                     EVLDPEQNSAFHDNYLDVDFDLSKVLFIATANNINTIPLPLLDRMELIEVSGYTQEEK
                     IEIARRHLIPKELENHG"
     gene            complement(1850..2329)
                     /locus_tag="PLR72_02850"
     CDS             complement(1850..2329)
                     /locus_tag="PLR72_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOK36180.1"
                     /translation="MRTFFITDQQEIENIIQQCDVCFVGITDEDGSPYVIPMNFAYRK
                     GEIILHSAPEGKHLRLLQQHPMVCITFCSERKLMYQHPDVACSYSMNSKSVLCKGEVS
                     FIEELSEKEEAMNLFMIKYTGRTFKYSLPALRNVKVWKIPIQEITAKAFGQNFKNKG"
     gene            2641..5697
                     /locus_tag="PLR72_02855"
     CDS             2641..5697
                     /locus_tag="PLR72_02855"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36181.1"
                     /translation="MKKIKYIILACLMSSAGIIFNATAQSSFGRNKPSYKTFKFEVLQ
                     TPHFEIYHYLKNDTILNYFAAWAEEWYRIHQIQFKDTFKLRNPIILYSNHADFQQTNI
                     ISDIISEGTGGVTESLKNRVILPFAPSLYQTDHVLGHELVHAFQYHKMLGSDSVRYSL
                     NNIPLWMVEGMAEYFSIGSVDANTSMWMRDAIINKDFPSIKDLTNTSKYFPYRYGQAL
                     WAFLGKTWGDTIIMPFFEKTALYGLEPAIDSVFHFNSKTLSGMWKAATENHYKQYMKD
                     TTYKTIGNKIISKTNAGSMNVCPSLSPDGKYLAFFSEKNIFTLDLFLADAKTGKIIKK
                     ISSINHQHEIDDFSFIESGGTWSPDSKKFAFVVFSKGRNKLAVVDIAKNKVKLIEMKG
                     IESFSNPEWSPDGKYIVFTGLIEGIGDLYLYNFETGEIEKITHDVFSNIHPSWSPDGN
                     YIVYATEKINRGGSKKFSFALSLLDMKTKKSKNLDIFPMADNLNPRFSNDGHFIYFLS
                     DADGFRNLYKYDLQTDKVYRLTEFMTGISGITLFSPAITTDRENNLIAYTYYFNKNYE
                     IYIASDHDFIANEVDKHSVNFDASTLPILKHVGPNIVDSLLYHRPPLLALPVDSVSKV
                     PYRPKFKLDYISNTAGIGISTGPVNTTEMGGSIFMIFSDMLSNHQLYTSLSLNGEIYD
                     FGGEVAYINQTNKVKWGTSLSHIPYRSGNMFWTIDTLTINQKDYLVVDNLVLDYIRLF
                     EDNISLFSYYPFSQTRRIEAALSASWYSYRIDRYHNYYDAWGDYLGGKREKLPAPSGT
                     NFQIASLAYVEDNSYFGMTSPLQGKRSRYQVDKYFGNINLFNLTLDYRKYFFSKPVGL
                     AFRIMHEGLYGSNSQIQLASPLYIAYPWLIRGYEDISFYSSSNYANYSLNVSNLAGSR
                     MVVGNVELRFPFTGPQVLAPIKSDIFLTDLNLFFDAGVAWNKGETVKFHWQPSKFDER
                     IPVCSAGVSLRINLFGYLVIEPYYAFPFQNGGFHNGTFGINFLPGW"
     gene            complement(6241..7440)
                     /locus_tag="PLR72_02860"
     CDS             complement(6241..7440)
                     /locus_tag="PLR72_02860"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 domain-containing protein"
                     /protein_id="HOK36182.1"
                     /translation="MNARQFPLLLLVIATVSSAAVEINLKVTNPNKKFVANAPVVIQL
                     QNLKNISSNERSQLAVYVDNKQISSQLDDLNKDGIQDELVFLLDLKGKETRKVVIKTI
                     PDSERQKFPPEVYADLILKTKDGEWQYLNEISSSKNDMYNKLHHHGVAFESALIGYRI
                     YFDNKSTVDIYGKKKYRLELPDTEWYPTSEQIAAGYGDDVLRVLQTVGVGTVKGFTNG
                     HTVHIDKFDKRTQRIVALGNLRAVVEWEVEGWEYEGEKINITARYILYARHRDVAVEV
                     RASENINQLATGVQRIDAGSLFSNNKGLVGSWGADYPVDDTIHYEKQTCGLGVYVPKK
                     YVLHHQIDDANNLVIMQYLKNDVLCFHFLAVAVTKEEENKIHSDKEFFTFLKQWSLNL
                     KPVQVKF"
     gene            7978..8601
                     /locus_tag="PLR72_02865"
     CDS             7978..8601
                     /locus_tag="PLR72_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOK36183.1"
                     /translation="MLKIILKGIIIGWFISGPIGPIGMLVIQRTMNRGIKYGIATGIG
                     AAFSDLIYTVITLFCLNFVIGFIEKNQLIIELIGSIIVIFFGYFIFKSNPALQPKPNE
                     PAHDNLFKDFISSFVLTFSNPLIIFVLIALFARLNFITRATNSFLILCGFLSIFAGAM
                     IWWLALTFLVSHFRSKVSIHQLKAINQITGSIIMLIGLVGTVVSFLK"
     gene            complement(8913..9683)
                     /locus_tag="PLR72_02870"
     CDS             complement(8913..9683)
                     /locus_tag="PLR72_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOK36184.1"
                     /translation="MQGIVTLSLVPLRVEDNECSEMYSQLLFGERVEILETRERWLKI
                     RNLADNAVGWIDRKMIEILDSIENESIESCYCTLVPIAEVRIINKNEKMLLPGGSLLP
                     DYENGKFHIQNNIFEVNPDEAGRPLKPSGNQLISLAKQYLNSPYLWGGKSALGIDCSG
                     LTQVIFSICGIFLPRNAYQQAEKGKQVPSLKEVHAGDLAFFQNSEGKIVHVGLLLNSH
                     QIIHCSGWVKIENIDSKGIVSSKTGKYSHSFHSIRRLI"
     gene            9877..11907
                     /locus_tag="PLR72_02875"
     CDS             9877..11907
                     /locus_tag="PLR72_02875"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOK36185.1"
                     /translation="MKLNFSKSSLQNIFKILLGSAVVIAIVLLFPKENKFNYHFEIGK
                     PWNYELLTAPFNFPVYKSEEQVIKEKNEILKNYIPYYVLDTTVYETQLKKLLRDNEIQ
                     LNNSVIARNYLREKLQFIYSQGIISSEEYNNLLKNNKTNIYCILPSRVSQIINIDKIY
                     TPQKAYEEIVKNSPEDILSLNLQKYLVENLRYDSITSELSKEDLLKNLSLTSGMVQRG
                     ERIIDKGEIVTPETYAILHSLKIEFDKRNSSIGQSFLVPMGEIIIIVELLILLIFYLN
                     LFRPRIFEKFNNLLFIAILMILIIGITSVVIRYTTLNYYLIPFALLPIIVRVFFDSRT
                     ALFVHIITILIISLMVDNPFQFVLLQITAGMVAVSSLKDMTQRSQLAQTAFYIFLSYT
                     LMYIGFEFITEKEWKRIDWIPILYFAVSCIFLLFAYLLIYIFEKIFGLISAITLIELT
                     NINSDFMMKFAEVAPGTFQHSLQVSNLATEAAKKINANSLLVRTGALYHDIGKMIHPE
                     FFIENQIGNKNPLLEMDYEKAAKTIIQHVTEGVKIAKKNHLPEQIINFIATHHGTSKT
                     KYFYNSFINQFPDIQPNEAAFTYPGPLPNNKETAILMMADAVEATSRSLNSYSEENIE
                     QMVEDIINGQINEGQFRESPISFRDVETVKAVFKEKIKNIYHSRIPYPEVEK"
     gene            11968..12966
                     /locus_tag="PLR72_02880"
     CDS             11968..12966
                     /locus_tag="PLR72_02880"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOK36186.1"
                     /translation="MKTKIAFFDAKSYDIATFNEKNKQYGFEIKYYKSHLNKDNAILS
                     KGADVVCIFVNAEANAEVIDMLVENGVKLIALRCAGYNNVDLKAAKGRIKVVHVPAYS
                     PHSIAEHTVALMLTLNRKIHKAYSRTREGNFALEGLMGFEMNGKTAGIIGTGKIGKIV
                     IQILKSMGMKILAYDAYPDHEFAKSIGIKYTTLNDLYKHSDIISLHCPLTKETEYLIN
                     YDSIEKMKDGVMIINTGRGKLINTKALIDGLKKKKIGYAGLDVYEEESDYFYEDYSNI
                     VIDDDILARLLTFNNVIVTSHQAFFTREALDEIARITLQNIQDFLDGKPLENEISI"
BASE COUNT         4215 a         2190 c         1991 g         4577 t
ORIGIN      
        1 aaaccatgat tttcaagttc ttttggaatc agatggcgtc gagcaatttc tattttttct
       61 tcctgtgtgt atccacttac ttcaatcagt tccattctat ccaacaatgg aagcggaata
      121 gtattaatat tgttggcagt agcaataaat agcacttttg acaaatcaaa atcaacatcc
      181 agataattat cgtggaaagc actattttgt tcagggtcga gcacttctaa caaagccgaa
      241 gaaggatcgc ctttataatc ggctgaaact ttatcaattt catccaatac aaatacagga
      301 ttagcagatt gtgctttttg aatattttga atgatgcgtc caggcagtgc tccaatataa
      361 gttcggcgat ggcctcttat ttcggcttcg tcgtgtaatc cgcccaatga aacacggaca
      421 tattttctat tcaaagccac tgcaattgat tttgccaatg aagttttccc tgttcccggt
      481 ggtccatata aacaaagaat aggagctttc atatcaccct tcagtttcaa tactgccaga
      541 tgttccagaa ttcgttcttt cacagtttcc agtccaaaat gatctttgtc aagaatttta
      601 cgagcatttt tcaaattaaa attatctttt gtatattcat tccacggcaa atcgagcatc
      661 atttcaacat aattcaactg aatggagtaa tcaggagaat aagtactcat tcgttctaac
      721 ttattgagtt ctttattaaa aactttagcc accgattcgt tccacttttt ttgctttgca
      781 cgttctctga attcaacaat atcttcttct ggtgaagtac ctcctaactc atcttgaata
      841 gtttttaact gttgttgaag aaaatattct ctttgttgtt tatcaatccc ttcttttgcc
      901 ttattttgaa tagaaatttt tatttccagc aattgtaatt ccttatttaa caaatccagc
      961 aattgataac ctctttcctt aatatcatca atttcaagca atctttgctt atcttccata
     1021 ttcaatccaa aattgacaca aatgtaattg attaaaaata ctggattatc aatattgcga
     1081 atagcaaaaa tattttcagg tggcatgacg cccgaattag aaataatttt tgtcgccacc
     1141 tcttttatgt tggaaatcaa tgccaaaaaa acagtatcgt cagtttcagg tattttttct
     1201 tctgctatct caatttttgc tttgaaaaaa ggtttttctg tctgaaattc ggcgatccga
     1261 aaacgtggtc ctccttccag aataacggta gtggtgttgt cattcatttc gaaaatttta
     1321 acaatttttg ctactgttcc caccttataa agttgttcag aagtaggatc ttcaatattt
     1381 ttatcttttt gagcacatac accaattagc aagtcgtttt ccctggcttt tttgactaat
     1441 cgtaaagatt tagcccgtcc tactgaaaca ggcaataaaa cacccggaaa aagtaccata
     1501 tttctcaacg gaaggatata gagtgtttct cctaagttta atttattatt ggatggatga
     1561 tcttcttgaa tactcataat cgggaaaatt tctgatgaat tttctaattc ttcaaaatca
     1621 tcaaatatat aattcatttc ttatcttatc gttttaattt ttatatgaca aaatgacaca
     1681 attatttttt ataaattttt acactatcaa atttttattg taaaagactt agattttcta
     1741 atatttcata gcagttcttt atttctcaat agttatgcca aaaatcaaca atttgttatt
     1801 ctctttggta ctgcttttat cgtatttatc agaaaataac aaaaaatgtt tatcctttgt
     1861 ttttaaaatt ttggccaaat gcttttgctg ttatttcttg gatgggtatt ttccaaactt
     1921 ttacatttcg cagagcagga agcgaatatt tgaaagttcg tcctgtatat ttgatcataa
     1981 agagattcat tgcttcctct ttttcagaaa gttcttcaat gaagctcact tctcccttgc
     2041 agagaacact ttttgaattc atactgtaac tacaagccac atcgggatgt tgatacatta
     2101 attttctctc tgaacagaaa gtaatgcaaa ccattggatg ctgctggagt aaccgtaaat
     2161 gtttcccctc aggagcagaa tgcaaaatga tttcaccttt acggtaagca aaattcattg
     2221 ggataacata gggacttcca tcttcatcgg taatgcctac aaaacaaaca tcacattgct
     2281 gaatgatatt ttcaatctct tgttgatcag tgataaaaaa tgttctcata tttctttaat
     2341 tatttgacaa agatacttaa aaggttgttt gaattttatt atctttttag tttttgagtt
     2401 ttttctttaa accttgctct gtcaaaatta tagcccgtgt tttataattt gtgaatttca
     2461 aatcctgtgc tatgatgatt tttcaatatt tgcatatttt tatttatcaa aaatggcact
     2521 ttttttgcct catctttaaa tgattaagtt tttttaaaat cgaagcaata aaaagaagta
     2581 tttatttttc tatctttatt ctattattat cattataaaa ttctttacgt agcaataaaa
     2641 atgaagaaaa taaaatatat aattttagct tgcttgatga gttcagcagg tataattttt
     2701 aatgcgacgg cacagtcatc tttcggtcga aataaaccaa gttacaaaac cttcaaattt
     2761 gaagtgttgc aaacacctca ttttgaaata tatcattacc tgaaaaatga taccatcctc
     2821 aattattttg ctgcctgggc tgaagaatgg tatcgtatac atcaaatcca atttaaagat
     2881 acttttaaac ttcggaatcc tattatcctc tattccaatc atgctgattt tcaacaaacc
     2941 aacattataa gcgacatcat tagcgaagga actggtggcg tgacagaaag tttaaaaaat
     3001 agagttattt taccttttgc gccaagtttg tatcagactg accatgtgtt aggacatgaa
     3061 ttggttcatg cttttcaata tcataaaatg ttaggctctg atagtgtacg ttattcactg
     3121 aataatatac ccctctggat ggtggaagga atggctgaat atttttcgat tggtagtgtt
     3181 gatgccaata cttcaatgtg gatgagggat gccattatta ataaagattt tccaagcata
     3241 aaagatttaa ccaatacttc caaatatttt ccttaccgtt atggacaagc attgtgggct
     3301 tttctcggaa aaacatgggg tgatactatc attatgcctt tttttgaaaa aactgcctta
     3361 tatggtttag agcctgctat tgattctgtt tttcacttta attcaaaaac tttatcgggc
     3421 atgtggaaag cggctactga aaatcattat aaacaataca tgaaagatac tacttacaag
     3481 actattggaa ataaaatcat ttctaaaacc aatgcaggtt ctatgaatgt ttgtccttct
     3541 ctaagtcctg atgggaaata tctggctttt ttttctgaaa aaaatatatt tacacttgat
     3601 ttgtttttag cagatgccaa aacaggaaaa attattaaaa aaatttcaag cataaaccat
     3661 cagcatgaaa tagatgattt tagttttatt gaatcaggag gaacatggtc gcctgatagt
     3721 aaaaaatttg ctttcgttgt ctttagtaaa ggacgaaaca aattagcggt tgttgacatt
     3781 gctaaaaata aagtaaaact tattgaaatg aagggaatag aatctttctc aaatcctgaa
     3841 tggtcacctg atggtaaata tatcgttttt acaggtttaa tagaaggaat tggtgatcta
     3901 tatctttata attttgaaac aggagaaata gaaaaaataa cccatgatgt attttctaac
     3961 attcatcctt cctggtcgcc tgatggtaat tatatcgttt atgcaactga aaaaattaac
     4021 cgtggtggct caaaaaaatt tagttttgca ttaagcttgc tggatatgaa aaccaaaaaa
     4081 agcaaaaatc tggatatttt tcctatggct gataatttaa atcctcgctt ttcaaatgac
     4141 ggtcatttta tttatttctt atctgatgct gatggattta gaaatttgta caaatatgat
     4201 ttacaaaccg acaaagtata tcgcttgaca gaattcatga caggcatcag tggtataacc
     4261 ttattttcgc ctgctattac aaccgataga gaaaataatt taatagccta tacttattat
     4321 ttcaataaaa actatgaaat ttatattgca agtgatcatg attttattgc gaatgaagta
     4381 gataagcata gtgtgaattt tgatgcttcc acgcttccta tattaaaaca tgtaggacca
     4441 aatatagtgg attcattgct ttatcatcga ccaccacttt tagcattacc ggttgattct
     4501 gttagtaaag taccttatcg cccaaaattt aaattagact atatctccaa tacggcagga
     4561 atcggaattt caactggacc tgtcaataca acagaaatgg gaggcagcat ttttatgatt
     4621 ttcagtgata tgttgagtaa ccatcaactt tatacttcct tgtcattaaa tggtgaaatt
     4681 tatgattttg gcggagaagt tgcctatatt aatcaaacca ataaagtaaa atggggaact
     4741 tctctttcgc atattcctta ccgaagtgga aatatgttct ggacaataga cactttaact
     4801 atcaaccaaa aagattacct cgtagtagat aatttggttt tagattatat tcgcttgttt
     4861 gaagataaca ttagcttatt ttcttactat cctttttcgc aaaccagaag aatagaagct
     4921 gccttgtcgg cttcgtggta ttcatatcga atagataggt atcataatta ttatgatgca
     4981 tggggtgatt acttaggagg aaaaagagaa aaacttccag caccatccgg aaccaacttt
     5041 caaatagcaa gcttggctta tgtagaagat aactcctatt ttggaatgac ttcaccgctt
     5101 caaggaaaga gaagtcgata tcaagtagat aaatattttg gaaatatcaa tttattcaat
     5161 cttacattag attatcgtaa atattttttc tcaaagcccg ttggtttagc ttttagaatt
     5221 atgcatgaag gattgtatgg aagcaattct caaattcaac tggcttctcc gttatatatt
     5281 gcttatccat ggctcattag aggttatgaa gatatttctt tttatagttc gtcgaattat
     5341 gccaattatt ctctcaatgt ttctaatctt gcgggttcga gaatggtggt aggcaatgtg
     5401 gaactgaggt tcccgtttac tggtcctcag gtattagctc ccatcaaatc agacattttt
     5461 cttactgatt tgaacttgtt ttttgatgct ggtgtagctt ggaacaaagg agagactgta
     5521 aaatttcact ggcagccttc taagtttgat gaacgaattc cagtttgcag tgctggcgtt
     5581 tctttacgta tcaatctttt tggttatctg gtaattgaac cttattacgc attccccttc
     5641 caaaatggag gatttcataa cggaaccttt ggaatcaatt ttctaccagg atggtaatta
     5701 tgtgaaaagg aacaagtaaa gacccttact tttcgaggta cgagtttaga gtccgtagct
     5761 ttttggccac acgattgtat cgtgtggcat cggtggggtc aaaattcaaa gaatatgact
     5821 gtcccgtgac attgatttat tcaaccttat tttgctgaca accttaataa caaaacaaat
     5881 cctcccaaaa gttaacctta ttaccttatt aaaaattgaa caaaataaga taataaggtt
     5941 gtaagctata ggaaacattc cctattatga aggttaaagg tttttgataa ggttttttta
     6001 tgacaaatat tggtttatcg aaatgtttga ttttatcagt ggttaaaaat cgggaaaatt
     6061 tatttttttc ttcagccctg tcgcacttac ttgcacaatg caaccgtgcg agagcaaaag
     6121 aatctttatt cgaaactctt tattcaaaac ttgcaaaacc cccacctcat atcttgaaac
     6181 tcgtacttct tattataaga aaattaaaaa atcaaagaag aaaaaataat ttttgacttc
     6241 tcaaaatttt acttgaacag gctttaaatt taaactccat tgttttaaaa aagtaaaaaa
     6301 ttccttatcg ctatgtattt tattttcttc ttcctttgtg actgcgacag caagaaaatg
     6361 aaaacaaagc acatcgtttt ttaaatactg cataattact aaattattcg catcatcaat
     6421 ttgatgatga agtacgtatt ttttaggaac atacactcct aaaccacatg tttgtttctc
     6481 ataatgtatt gtatcgtcaa caggatagtc ggctccccac gaacctacca aacctttatt
     6541 attactgaat aaacttcctg catcaatacg ttgtacgcct gtagccagtt gattaatatt
     6601 ttcggaagct cttacttcca cagcaacatc ccggtgacga gcatataaaa tatagcgagc
     6661 agtgatattt attttttccc cttcgtattc ccaaccttct acttcccatt ccacaactgc
     6721 tcgcaggttt cctaaagcaa caattcgctg agtacgttta tcaaatttat caatatgaac
     6781 agtatgtcca tttgtgaatc ctttcacggt accaactcct acggtttgta aaacacgtaa
     6841 cacatcatct ccatatcctg ctgcaatttg ttcagaggta ggataccatt cagtatcagg
     6901 aagctcaagc cgatatttct tttttccata tatatctact gtgcttttat tgtcgaaata
     6961 aatcctgtag cctattaaag ccgattcaaa tgctactcca tgatgatgga gcttattata
     7021 catatcattt tttgaagacg aaatttcatt taagtattgc cattccccat cctttgtctt
     7081 taaaattaaa tcagcataaa cttcaggggg aaatttttgt cgttcactat caggtatggt
     7141 ttttataacc accttacgcg tttctttacc ttttaaatcc aataaaaaaa ccaattcatc
     7201 ctgaattcca tctttattta aatcgtccag ttgactgctt atttgtttat tatcaacata
     7261 aacagccaat tgacttcttt catttgaaga aatgttcttc aaattttgca actgaattac
     7321 tacgggtgca ttggcaacaa attttttatt gggattggtc acctttaaat ttatttcgac
     7381 agcagcagaa gaaacggttg ctataacaag caataataaa ggaaattgtc gtgcattcat
     7441 ttttgtaaaa ttttaaaagt ttcctgtaaa attagttttc taattgataa atttcaataa
     7501 tttttttcta taaaagaggt aaaaattgac ataatatgag tatttttttg tttttaattt
     7561 tatttgtcgc attataagtg aatggaatta gataaaattg agtctttata ataattatcc
     7621 gcaattcatt aggggacaaa ttttatttga aataatataa cgttactttc ccaaatgaac
     7681 agttgtttcg gcaatttcgc ttcgttttaa ttatctaaca tcaatctatc taaattatct
     7741 tgtaagaatt ttttctatgc aactctatac tttccctgtt gaactatttg aaaatgaata
     7801 atttatcaca ctgagaaccc cttatatttt ttttcttcta ttttactgaa gacgtttctt
     7861 gaaacgtttc cactcgaaac ttcaaacaat taaaaagtgt tgattttttt tattccgttt
     7921 ttttaatgaa aaagaatgat ttatctttgc cccttcaaac cgagaaagtt aagataaatg
     7981 ttaaaaatta tcctcaaagg tattattatt ggttggttta tttcaggacc cattggtccc
     8041 attggtatgc tggttattca acgtactatg aatagaggta tcaaatatgg tattgcaacg
     8101 ggaattggtg ctgctttttc tgatttaata tatactgtta tcactttatt ttgcctgaat
     8161 tttgttatag gctttattga aaaaaatcaa ctcatcattg agcttattgg cagtataata
     8221 gtaatttttt tcggttattt cattttcaaa agcaatcctg ctcttcaacc caaacctaac
     8281 gaacctgccc acgataatct ttttaaagat tttatcagtt cctttgtact tactttttcc
     8341 aaccctttga tcatttttgt tttgattgct ttgtttgctc gtttgaattt tattactcga
     8401 gcaactaatt ctttccttat cctatgcggt ttcctttcta tttttgctgg tgcaatgatt
     8461 tggtggttag cattaacttt tcttgtaagt cattttcgtt caaaagtcag tattcatcag
     8521 ttgaaagcta taaatcaaat tactggaagc atcattatgt taattgggct cgttggcact
     8581 gttgtaagct tcttaaaata attcaaccaa ctactttaaa ggaagtggtt gaaaatcagc
     8641 ctattgaaaa taacaaaact gtaactttac aaaatagcat ctttgtattg taaaaagttt
     8701 tgttagaata acttagacat ttcttctttt gtcattttgt cattttgcca ttcaaccaga
     8761 taatggacat ctcatttttt ttcctttgtc ttgaaaaata attttccttt atcttttatt
     8821 tcccctgaaa aagttgcatt aattacttga atttacgttc taaaaaaata agaaatgatg
     8881 aaattatcaa gatgaaaata accaatgggt tattaaatca atcgtctgat agagtgaaac
     8941 gagtgagaat attttccagt ttttgaagaa acaatacctt ttgaatcaat gttttcaatt
     9001 ttaacccagc cagaacagtg aataatttga tgagaattga gcaaaaggcc aacatggaca
     9061 attttccctt cactgttttg aaaaaaagcc agatcaccgg cgtgaacttc tttcaaagaa
     9121 ggaacttgtt tacctttttc tgcttgctga taagcatttc gtggtaaaaa aatgccacat
     9181 atggaaaaaa ttacttgcgt caatcctgaa caatctattc ctaaagcaga ttttccgccc
     9241 cataaataag gtgaattcaa atattgttta gccaacgaaa taagctgatt cccagaaggt
     9301 ttaagtggtc gacctgcttc atctggattt acttcaaaaa tattgttttg tatgtgaaac
     9361 tttccatttt cataatctgg aagtaaactt ccgcctggta aaagcatttt ttcatttttg
     9421 tttatgatcc gaacttctgc aataggtaca agtgtgcaat aacaagattc aattgattca
     9481 ttttcaatgg aatccaggat ctcaatcatt tttctatcta tccaaccaac agcattatcc
     9541 gctaaatttc tgattttcag ccagcgttct cgcgtctcaa gaatttcgac tcgttcacca
     9601 aacaaaagct gcgaatacat ctcgctgcat tcattatctt caactcttaa aggcaccaaa
     9661 cttaatgtta caattccttg catttttgac ttacattaaa agcaatattg tatctttatt
     9721 tttaattctt tatcacaaaa ttacagaaag tttataaatt tatcttttca tcttgaaata
     9781 ttttgtaatt ttcgccttct aattattaat aacttcaaac taatttatac tttagtgttt
     9841 tctaagttct gttagaaaaa tagatactca ataattatga aattaaattt ttcgaaatct
     9901 tcactacaaa atatttttaa aattttattg ggttctgctg tagtaattgc aattgttttg
     9961 cttttcccga aagaaaataa attcaattat cattttgaaa taggcaaacc gtggaattat
    10021 gaactattga cagctccttt caatttcccg gtttacaaaa gtgaagaaca agtaatcaaa
    10081 gaaaaaaatg aaatcttaaa aaattatata ccttattacg tccttgatac taccgtttat
    10141 gaaactcagc ttaaaaaatt gcttcgtgac aacgaaattc aattaaataa ctctgtcatc
    10201 gcaagaaatt acttgagaga aaagctacaa ttcatttatt ctcaaggaat tatttcttca
    10261 gaagaatata acaatttgct gaaaaataat aaaacaaata tttactgtat tctaccttcg
    10321 agagtttctc aaataatcaa tatagataaa atttatacac ctcaaaaggc ttatgaggaa
    10381 atagtaaaaa attcacctga ggatatttta tcactcaatc tacaaaaata tctggttgaa
    10441 aatttacgat acgacagtat tacttctgaa ctttcaaaag aagatttact aaaaaacctt
    10501 tcccttacta gtggaatggt acaacgagga gaacgcatca tcgataaagg agaaatcgtc
    10561 acccctgaaa catacgctat tttacattcg cttaaaattg aatttgataa aagaaattct
    10621 tctattggac agtcattttt ggtacctatg ggtgaaataa ttattattgt ggagctgcta
    10681 attttgctga tattttacct taaccttttc cgtccacgta tttttgagaa atttaataat
    10741 ttgcttttca tcgccatctt aatgattttg ataatcggca ttacctctgt tgtgattcgt
    10801 tataccacac taaattatta ccttattcct tttgctttgc tgcctattat tgtacgagta
    10861 ttctttgatt cccgtactgc tttatttgtg catattatca ctattctcat catttcattg
    10921 atggtggaca atccttttca atttgtattg ttgcagatca ccgcaggaat ggttgcggtt
    10981 tccagcctga aagatatgac tcaacgttca caattagctc aaacagcctt ttatatcttt
    11041 ctgagctata ctttgatgta cataggtttt gaatttatta ctgaaaaaga atggaaacga
    11101 atcgactgga tacctatttt atattttgct gtaagttgta ttttcttact tttcgcttat
    11161 ttgttgattt acatttttga aaagattttt ggattaattt cagctattac gttgatagaa
    11221 ctaaccaata tcaatagcga ttttatgatg aaatttgccg aagtggcgcc tggcactttt
    11281 caacattctc tgcaagtttc taaccttgcc actgaagctg caaaaaaaat caatgccaat
    11341 agtttactgg tacgcacggg agctttgtat cacgatattg gcaaaatgat tcatcctgaa
    11401 ttttttattg aaaatcagat aggaaataaa aatccacttt tggaaatgga ttatgaaaaa
    11461 gcagccaaaa caatcattca acatgtgact gaaggggtta aaattgccaa aaagaatcat
    11521 ttgcctgaac aaattatcaa ttttattgcc actcatcatg gcactagtaa aacaaaatat
    11581 ttttataatt ctttcattaa ccaatttcca gatatccaac caaatgaagc agcttttact
    11641 tatcctggac cgcttcccaa caacaaagaa acagctatcc tgatgatggc tgatgcggta
    11701 gaagccactt caagaagttt aaactcttac agtgaagaaa acatagaaca aatggtagaa
    11761 gatatcatca atggacaaat caacgaggga caatttaggg aatctcccat tagtttcaga
    11821 gatgttgaaa cagtaaaagc cgttttcaaa gaaaaaataa aaaatattta tcattcacgg
    11881 attccctatc ctgaagttga aaaataagct attgctcaat catttccaca aacaatactt
    11941 gtaatgtata aataaataac ttaaataatg aaaacaaaaa ttgccttttt cgatgcaaaa
    12001 tcctatgaca tcgccacttt taacgagaaa aataaacaat atggctttga aataaaatat
    12061 tataaaagtc atttaaacaa agataacgcc attctatcca aaggagccga tgtagtgtgc
    12121 atttttgtaa atgccgaagc caacgccgaa gtaatagaca tgttggtaga aaacggcgta
    12181 aaattaatag ctctccggtg tgctggttac aacaatgttg atctgaaagc agcaaaagga
    12241 cgtatcaaag tggttcatgt tccagcttat tcaccccatt ctattgcaga acatacagta
    12301 gcattgatgt taactttgaa ccgaaaaatt cacaaggctt attccagaac gcgtgaggga
    12361 aattttgcct tggaaggatt gatgggattc gaaatgaacg gaaaaacggc tggcattatt
    12421 gggacaggaa aaattgggaa aattgttatc cagattctga aaagcatggg aatgaaaatt
    12481 ctggcttatg atgcttatcc cgatcatgag tttgccaaaa gtataggaat aaaatatact
    12541 actttgaatg atctttataa acattcagat attatttcat tgcactgtcc attaacaaaa
    12601 gaaacagagt atctgatcaa ttatgactcc attgagaaaa tgaaagatgg cgtaatgatt
    12661 attaatactg gaagaggaaa gcttatcaat acaaaagcat taattgatgg acttaaaaag
    12721 aaaaaaatag gttatgctgg tctggatgtg tatgaagaag aaagtgatta tttttatgaa
    12781 gattattcca atattgttat tgatgatgat attttggctc gactgcttac tttcaataat
    12841 gtaattgtta cttctcatca agctttcttt accagagagg ctttagacga aattgctcgc
    12901 attactttgc aaaatattca ggatttttta gacgggaaac cattggaaaa tgaaatcagt
    12961 atttgaaatc aaa
//
LOCUS       DAOSAS010000038        12896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3277_length_12896_cov_12.906316, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000038 DAOSAS010000000
VERSION     DAOSAS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12896
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3277_length_12896_cov_12.906316"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..567)
                     /locus_tag="PLR72_02885"
     CDS             complement(391..567)
                     /locus_tag="PLR72_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36187.1"
                     /translation="MRKLDLRHRPTFLFTYLQPALENGWIEMTIPNNPNDPNQKYRLT
                     QKGETLQSQLKNKK"
     gene            1039..2016
                     /locus_tag="PLR72_02890"
     CDS             1039..2016
                     /locus_tag="PLR72_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOK36188.1"
                     /translation="MKKNLIIVLALIVFISCTASKKKNEKEAEKMNIPTFSSDSAYLY
                     IEHQTNFGPRVPNSSAHAACQQYLFHSLKRFGAEVIIQQADLQAFDGTILHAANIIGS
                     FSPAKENRLLLFAHWDSRPWADNDPEPANRRQPVLGADDGASGVGVLLEIARQLGKQL
                     PNIGVDIIFFDAEDYGNSQSFQNENTNNSWCLGSQYWAKHPHKQNYQARFGILLDMVG
                     ASQALFYKEYFSLTYAPQIVSKVWTEAKNLGFGQYFIDQQGGAITDDHFYVNNLRGIP
                     SIDIIHYDPNSATGFPPYWHTLHDTMENIDKNTLLAVGTTLLHIIYNEK"
     gene            2026..3108
                     /gene="proB"
                     /locus_tag="PLR72_02895"
     CDS             2026..3108
                     /gene="proB"
                     /locus_tag="PLR72_02895"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOK36189.1"
                     /translation="MELRFKKIVVKIGSNVLTQSDGTLDISIMSTLVDQIALLYKEGV
                     EIIIVSSGAVASGRSLLGINKKMDIVDQRQLYSTVGQAKLINLYWDLFQKHGITVGQV
                     LTMKQNFNSRRNYLNQRNCIKVMLENKIIPIINENDAVSVSELMFTDNDELSGLIASM
                     MDTEALFILSNIDGIYTGSVDDPSAKIIREIKKGEDISPYIQKTKSTFGRGGMQTKIN
                     IARKIADQGIEVFIANGRKENILIQLMDKNQEAICTHFVAEKSEVSSIKKWIAHSEDF
                     AKGEIYVNADAAKALKSDKAISLLPVGVTKIVGNFEKDDIVRIMDENGYSFGIGKIRY
                     DSTKARELIGKKHQKPLIHYDYMYLE"
     gene            3254..5509
                     /locus_tag="PLR72_02900"
     CDS             3254..5509
                     /locus_tag="PLR72_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOK36190.1"
                     /translation="MRKTERYLCILLVSVFFVGCNTTKFVPEGKYLLDKVDIKTDVRS
                     IKKNELYDYLRQTPNNSAFGLFRTQLGIYSLAGKDTTKWLNRFLMKIGEEPVIFNPTL
                     TDLSAQQLQLCFANKGYLNSKVDTKIEFYDKKARVEYIITSNQPYRIKNYNVQIPHNE
                     LAKIANDTLRSLIHRNMLFDIDVLDAERERITSRFRQMGYYNFRKDFLSYTADTTIAS
                     HQVDLTLHLNDNLLNPTDSLYSIIFKKFSIRNVTFYINATNISELQENGVTDTINFRN
                     FTMVTPRKQIIRIDALVQNTYINPKSVYNDVDVEKTYSALNSLGAIKYVNISFSETKD
                     SLLDCNIFIMPSSAISLTGEIEATYTDGYWGGAVNLNLINKNIFKGAETFSLQGHGAY
                     EWQKNIWAQEWGGQIGLKFPRFILPFASYDFKRNLHANTEFSGSYYYQFRPGEFRTTN
                     SQIGIKYLWNRSQYQHSINLIDINYVYFPEIFPTFREKFLDTGIFNRYNYENHFIMGI
                     GYSGSTTNYRLSSPLRNFFTMRYNIETAGNFLYGLNHLLGSAPKNGSYQLFNIDYAQY
                     VKTEYNITYHQIFDSNNRFVYHAGIGVGAPYGNGKIIPYEKRFFSGGANSVRGWTEST
                     LGPGVYKRINTLMRDYNQTGDVKLDLNMEYRAKLFWLLEGAAFIDAGNIWTIKDYETQ
                     PGGVFQWNNFIDQIAIAYGIGLRFDLTIMIARLDLGIKLHDPVLKRSECWRLPPKWSD
                     LAFHIAIGYPF"
     gene            complement(5490..6122)
                     /locus_tag="PLR72_02905"
     CDS             complement(5490..6122)
                     /locus_tag="PLR72_02905"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014709019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOK36191.1"
                     /translation="MTPELKSRFSFHKYECGCDEAGRGCLAGPVVAAAVILPDDFVCP
                     QINDSKKLTSGVREKLRFIIEKEALAWAVAEVSNVEIDQMNILNASILAMHKAIDKLS
                     IRPEFIIIDGNYFKPYADIPYQTIVKGDGKYLSIAAASILAKTHRDEIIEQLGEKFPQ
                     YYWQKNKAYPTFQHREAILKFGATPYHRMSYNLLGKKKHKKFDNFKKDNQ"
     gene            complement(6176..6667)
                     /locus_tag="PLR72_02910"
     CDS             complement(6176..6667)
                     /locus_tag="PLR72_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOK36192.1"
                     /translation="MNTLDLIILIPIVFGFIQGLFKGLIKELTSFAVIVIGIYGAKLL
                     TTPFEKLLLNIAFLSPQITKPLAFLLGFIIIAIGLFILAHLLDKTLSFIQLGGLNKFF
                     GGIVGGLKYLLILSILLNVFDIVDNHFHLINKTTKEKSLTYSKILKVAPVLWEEVKDK
                     KID"
     gene            complement(7231..8223)
                     /locus_tag="PLR72_02915"
     CDS             complement(7231..8223)
                     /locus_tag="PLR72_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOK36193.1"
                     /translation="MVSYKELGLVNSRELFKKAIEGGYAIPAFNFNNMEQLQAIISAC
                     VETKSPVILQVSSGARKYANQTLLRYMAQGAVEYAKELGYPIPIVLHLDHGDSFELCK
                     SCIEFGFSSVMIDGSHLPYEENVALTKKVVDYAHQFDVTVEGELGVLAGIEDDVKAEH
                     HTYTDPDQVVDFVSRTGVDSLAISIGTSHGAYKFKPEQCTVDANGRLVPPPLRFDILK
                     EIERRIPGFPIVLHGASSVPQEYVDEINRYGGQLKDSIGVPEEQLREAAKSAVCKINI
                     DSDGRLAMTAAIRRILWTQPAEFDPRKYLGPARDELKKLYMHKTQNVLGSAGKA"
     gene            complement(8350..9570)
                     /locus_tag="PLR72_02920"
     CDS             complement(8350..9570)
                     /locus_tag="PLR72_02920"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOK36194.1"
                     /translation="MAFINENFLKIPENYLFSEIAKRVAEHKAKNPSAKIIRMGIGDV
                     TLPLPDVVVDAMIKAAEEQRHKETFRGYGPEQGYEFLREAIAQHDFQKRGIKIEVDEI
                     FISDGAKSDTGNIGDIFHINNRVAVTDPTYPVYVDTNAMAGRAGEPDEHNQWSNLVYL
                     PCNKENNFVPALPKEKVNLIYLCYPNNPTGTTLTKEQLKVWVDYARENQAVILFDAAY
                     EAFITENNVPHSIYEIEGATEVAIEFRSFSKTAGFTGTRCGYTIIPQQVMGYTQKGEA
                     VSLNKLWLRRQSTKFNGTSYIVQRGAEATFTPDGRKQVKALIDYYLENARIIREGLTK
                     NGFAVYGGINSPYVWVKTPNGMSSWEYFDFLLKEKNIVTTPGAGFGISGEGYIRFSAF
                     GSKEDTIEAMKRLA"
     gene            complement(9633..10472)
                     /gene="dapF"
                     /locus_tag="PLR72_02925"
     CDS             complement(9633..10472)
                     /gene="dapF"
                     /locus_tag="PLR72_02925"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOK36195.1"
                     /translation="MKFTKMHGIGNDYIYINGFEEKIKNPNELSIRLSDRHKGIGADG
                     LVLILPSSVADFQMQMFNADGSEGEMCGNAARCIGKYVYEKGLTDKTELTLETLAGIK
                     KLKLFLNDKQKVEKVTVDMGEPILEAEQIPVLFQKSPIVNEPVDFGNGINYAITCVSM
                     GNPHAVIFTDNIDQLDIKTIGPIIENSSIFPKRTNVEFVEKISENILKMRVWERGSGE
                     TMACGTGACAVVVAGVLNKISSRKTTVKLLGGELEIEWNNNDNHVYLTGPAEMVFEGE
                     IDL"
     gene            complement(10650..11669)
                     /locus_tag="PLR72_02930"
     CDS             complement(10650..11669)
                     /locus_tag="PLR72_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOK36196.1"
                     /translation="MKCFASDNYSGIHPEILNAIIKANVDHETSYGEDSYTMQAQKVF
                     ESIFGDVDVLYAFNGTGANIVSLKCCTLPFQAVICAETAHINTDECGAVAQNIGCSLL
                     TVPTPDGKLTPEMIEPFLYRIGNVHNSQPKVISISQSTELGTIYSVSELKALCKFAHE
                     NNLYVHIDGARISNAVAALNVGLKEATVDCGVDIMSFGGTKNGLMLGEAILIFNSELR
                     KNAAFYQKQSMQLYSKNRFIAAQFIAFLSNNLWLQLALHSNQMAQMMAEKLQTIPEIK
                     ITQPVQTNSIFVIIPEKIIEPLREKYKFYTWNEQTNELRWMCSFDTIEKEIDDFVETI
                     RQLVE"
     gene            complement(11718..12491)
                     /locus_tag="PLR72_02935"
     CDS             complement(11718..12491)
                     /locus_tag="PLR72_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOK36197.1"
                     /translation="MYLTGLLILGIVAGILSGLFGIGGGIVLVPCLLVIFGLDILHAN
                     ATSLTAMLLPVGILGVWEYYKAGQINIKKSLWIALGLFLGSFVGGEIAVNISETFLTR
                     LYAFILLCIAISYFDLPKYFHKKNEIIVKEDPVISKSFWMYILVGVAAGIFAGLFGKG
                     GGIIIVPLLIHFFQYDAKKAVATSLASLQLPVGLPSVIIYAKEGYLNLLFAALIAGGM
                     VVGAFLGSKIGIRISSEIFKKVYGVFLLVVAIYLAIKYI"
BASE COUNT         4357 a         2234 c         2042 g         4263 t
ORIGIN      
        1 tgcttcaaaa aacatttcat ttttagtggt tcccgtaatt tcgctttact ttaattgtct
       61 tgacaaaaaa gaaaacattt tttcattttg cttgtttttg agattttttc tccgtacaac
      121 tcctgtcttt tctgtggaac tctgtgaaaa taactgatat attacataaa ggaactcgga
      181 gatttacaca gaaagatact gagatttttg ttcccctttt attttatagg agacgtttta
      241 tgaaacgtct ctacttaaaa ctccaaactc ctgattttca actaaatgtt caaaactcga
      301 aactacacat tgatataaaa aatatcttct ttgttggata ttaaaaaaac gaaataaata
      361 ttattggttg tttaaaaact gtctcctttt ttattttttg tttttcaatt ggctttgcag
      421 cgtctctcct ttttgagtca ggcggtattt ttgatttgga tcatttggat tatttggaat
      481 agtcatttct atccatccgt tttcaagtgc aggttgcaaa taagtaaaaa gaaaagttgg
      541 acgatgccgt aggtctaatt tcctcatcaa ctcttgccta ctaagttcgc cattaacaat
      601 cagaataaga cgtttaactt gttcagttac ttgttcagtt acttgttcgg taacttgttc
      661 ggtaacttgc tgactttctt cagattttct ccaaagaata gcgtaaaaac attatggagg
      721 agaaatacat ttaaatgttt agctatactg gataagcaac ctaatcaatg aacatactta
      781 tatacaaaaa taataaaatt taggaatctc taatgcatat ataagatttt ctatttttgt
      841 taaaaagtta ttgcttaatt taaattttcc tttcaaatct cgtttattct taaaaatctg
      901 ttgagttaat gaaaagttga ttttcttttt catgggtaaa ttgaaaatat catgtaaatt
      961 tgtaacttgt atttttagat tgatttttca ttggaatttt aacaactgtc ttttcaaaaa
     1021 aattaaattg cttatcaagt gaaaaaaaat ttgatcattg ttctggcatt aatcgttttt
     1081 atttcctgta ctgcttctaa aaagaaaaat gagaaggaag ccgaaaagat gaatattccc
     1141 actttttcga gcgattcagc ctatttatat atagaacatc aaacaaattt tggtcctcga
     1201 gttccgaatt cttctgctca tgcagcatgt caacaatatt tatttcattc tttaaaaaga
     1261 tttggggcag aagtaataat tcaacaagcc gatttacagg cttttgatgg taccattctt
     1321 catgcggcaa atattattgg ttctttttct ccagcaaaag aaaatcgcct tttgcttttt
     1381 gctcactggg attcgcgacc gtgggctgat aatgatccag aaccagcaaa tcgtcgacaa
     1441 cctgttttgg gtgctgatga tggtgccagc ggagtgggtg ttttattaga aatagctcgt
     1501 caattgggaa aacagttacc taacatagga gttgatatta tctttttcga tgcagaagat
     1561 tatggtaatt cccagtcatt tcaaaatgaa aatacaaaca attcgtggtg ccttggttct
     1621 caatactggg caaaacatcc tcataaacaa aattatcaag ctcgatttgg tattttactc
     1681 gatatggtag gagcatcgca agcccttttt tataaagaat acttttcgtt aacttatgct
     1741 ccacaaattg tttcaaaagt gtggacagaa gcaaaaaatc ttggctttgg tcaatatttt
     1801 attgatcagc aaggtggagc tattaccgat gatcactttt atgtgaataa tttaagagga
     1861 ataccgagca ttgatattat tcattatgat cctaatagtg ctaccggttt tccaccttat
     1921 tggcatactt tacacgacac tatggaaaat atagacaaaa atactctact ggctgtcgga
     1981 acaacgttgt tgcatatcat atacaacgaa aaataaaaat tgaatatgga acttcggttt
     2041 aaaaaaatag tagtaaaaat tggaagcaat gttttaactc aatcggatgg tacgttggat
     2101 atttccatta tgtctaccct tgttgatcaa attgccctat tgtataaaga aggtgttgaa
     2161 attattattg tttcgtctgg tgcagtagct tctggaagaa gtttattagg gatcaacaaa
     2221 aaaatggata ttgtggacca acgtcaattg tattctactg tcggacaggc aaaacttatc
     2281 aatctttatt gggatttgtt tcaaaaacat ggtataacag ttggtcaagt attaactatg
     2341 aaacaaaatt ttaatagtcg acgcaattac cttaatcagc ggaattgtat taaggtaatg
     2401 ctcgaaaata aaataattcc tatcattaat gaaaatgatg ctgtttctgt ttctgaacta
     2461 atgtttaccg ataatgatga actttcagga cttatagctt cgatgatgga tacggaagct
     2521 ttattcattt taagcaacat agatggaatt tataccggtt cggtcgatga tccttccgca
     2581 aaaattatcc gtgaaataaa aaaaggggaa gatatttctc cctacattca aaaaactaaa
     2641 tctacttttg gaagaggcgg tatgcaaaca aaaattaata tcgctcgtaa aattgccgat
     2701 cagggaatag aagtttttat tgctaatgga agaaaagaaa atattctcat tcagttaatg
     2761 gataaaaatc aggaggctat ttgcacacat tttgtggctg agaaaagtga agtttccagt
     2821 ataaaaaaat ggatagctca cagtgaggat tttgcaaagg gtgaaattta tgttaatgct
     2881 gatgctgcaa aggctttgaa aagtgataaa gcaataagtt tgttgcctgt gggtgttaca
     2941 aaaattgttg gcaattttga aaaggatgat attgtcagaa tcatggatga aaacggttat
     3001 tcatttggga tcggaaaaat tcgttacgac agtacaaaag caagagaatt aataggcaaa
     3061 aaacatcaaa aaccattgat tcattatgat tatatgtatc tggaataaaa taaaccgttt
     3121 ttgtggttta tgattttatt ccatcttttt gtacttataa aaagaattga agatatatct
     3181 ttccttattt tatgaagctt aacgatagca attgatgagg aattatttct aaagaaatca
     3241 aaatatctca tttatgagaa aaacagaaag atatttatgc attttgttgg ttagtgtatt
     3301 ttttgtggga tgcaacacga caaaatttgt tccggaagga aaatatttat tggataaagt
     3361 tgatataaaa acagacgttc gttctattaa aaaaaatgag ttatacgatt atctccgaca
     3421 aacgcccaac aattctgcct ttggattatt tcgtacccaa ttaggaattt acagtttagc
     3481 aggcaaagat actacgaaat ggctcaatag atttttaatg aaaataggtg aagaacctgt
     3541 tatttttaat cctactctta ccgatttatc tgctcagcag ttacaacttt gctttgccaa
     3601 taaggggtat ctgaattcaa aagtggacac gaaaatagaa ttttatgaca aaaaagcaag
     3661 agtagaatat atcattacct ccaatcaacc ttaccgcatt aaaaattata acgttcaaat
     3721 tcctcataat gaattagcta aaatcgccaa cgacactttg cggagcttga ttcatcgcaa
     3781 catgcttttt gatattgacg tactggatgc tgaacgcgaa agaattacct cacgatttcg
     3841 tcaaatgggt tattacaatt tccgaaaaga tttcctttct tatactgctg atactactat
     3901 tgcttctcat caagtagatt taactcttca tttaaacgat aatttactca atcccaccga
     3961 ttcactttat agtattatat ttaagaaatt cagtatacga aatgtaactt tttatattaa
     4021 cgccaccaat atttcggaac tacaagaaaa tggagttaca gacaccatca atttcagaaa
     4081 ttttactatg gttacgcctc gcaaacaaat tatcagaatt gatgctttgg tacaaaacac
     4141 ctatattaat cccaaaagtg tgtataatga tgtggatgta gaaaaaactt attctgcgct
     4201 taattcgctc ggagctataa aatatgtcaa tattagtttc agtgaaacaa aagatagttt
     4261 attagattgt aacattttta taatgccttc ttcagctatt tctcttacag gcgaaattga
     4321 agctacttat acagacggtt actggggtgg agcagtaaat ttgaacttaa tcaacaaaaa
     4381 tattttcaaa ggagctgaaa ctttttcatt acaaggtcat ggagcctatg aatggcaaaa
     4441 aaatatatgg gcacaggaat ggggtggaca gataggacta aagtttccac gtttcatatt
     4501 gccctttgcc agctatgatt ttaaaagaaa tctccatgcc aacactgagt tttctggatc
     4561 ctattattat caattcagac caggtgaatt tagaacaaca aattcacaaa ttggtattaa
     4621 atatctttgg aatcgttctc aatatcaaca ttctattaat ttaattgata ttaattatgt
     4681 ctattttcct gaaattttcc ccacttttcg tgaaaaattt cttgacacag gaatttttaa
     4741 tcgttataat tacgaaaatc acttcattat gggaatcggt tattctggtt ctactactaa
     4801 ttatcgtttg tccagtcctt tgagaaattt ttttaccatg cgatacaata ttgaaactgc
     4861 tggaaatttt ttatatggct taaatcattt attgggaagt gctcctaaaa atggttctta
     4921 tcaattattc aatattgatt acgcacaata tgtaaaaact gaatacaata ttacttacca
     4981 tcaaattttc gattcaaaca acagattcgt ttatcacgca gggattggcg tgggtgctcc
     5041 ttatggaaat ggaaaaataa tcccttatga gaaacgattt tttagcgggg gagcaaatag
     5101 cgtaagagga tggaccgaaa gcactttggg accaggcgtt tataaaagaa tcaatacgct
     5161 catgagagac tataaccaaa cgggtgatgt taagctggat ttgaatatgg aatacagagc
     5221 aaaattattc tggctacttg aaggagctgc ttttatagat gcaggaaata tctggactat
     5281 taaggattat gaaactcaac ctggcggggt ttttcaatgg aacaatttta tagatcaaat
     5341 tgcaattgct tatggaatag gacttagatt tgaccttact attatgatag cacgtcttga
     5401 tttaggaata aaactacatg atcccgtcct taaacgtagt gaatgttgga gactaccgcc
     5461 aaaatggagc gacctcgcct ttcatattgc tattggttat cctttttaaa gttatcaaat
     5521 tttttgtgtt ttttctttcc taaaaggtta tacgacattc gatgataagg agttgcccca
     5581 aattttaaaa tggcttctcg atgttgaaaa gtaggataag ccttattttt ttgccaataa
     5641 tattgaggaa atttttctcc caattgttct ataatttcat cacggtgtgt tttggctaat
     5701 atggaagctg cagctataga caaatatttg ccatcacctt tcacaattgt ttgataagga
     5761 atatcagcat aaggtttaaa ataattccca tctatgataa tgaattcagg tcgaatcgaa
     5821 agtttatcta ttgctttatg cattgccaaa atagaagcat tgagaatatt catctgatca
     5881 atttcgacat tacttacttc agctactgcc cacgccaaag cttccttttc tataatgaaa
     5941 cgtaactttt ctctcacacc ggaagttaac tttttagaat catttatctg aggacaaaca
     6001 aaatcgtcag gcaaaattac tgcagcagct acaactggac ctgccaaaca accacgacct
     6061 gcttcatcac atccgcattc atacttatga aatgaaaaac gggactttaa ttcgggagtg
     6121 attttatttc tttttgttag attcaatttt gttttttgaa taaatatttt tctgatcagt
     6181 cgattttttt gtctttcact tcttcccaca aaacaggagc aactttcaga attttagaat
     6241 aagtcaatga cttttctttg gtagttttat taattaaatg aaaatgatta tcaacgatat
     6301 caaaaacatt caacaagata ctgagaataa gaagatattt taagccacca acaataccgc
     6361 caaaaaattt gttcaaccca cctaattgaa tgaacgataa tgttttatcc agtaaatgag
     6421 caagtataaa caagcctata gctataataa taaaacccaa taaaaaagcc aaaggtttgg
     6481 ttatctgggg ggacaaaaat gcaatattaa gcaacaactt ttcaaaggga gtggtcaata
     6541 attttgctcc ataaatacca atgacaatga cagcaaagga ggttaattct tttatcaatc
     6601 ctttgaataa accttgaata aaaccaaaaa caataggaat caaaataatt aaatccagtg
     6661 tattcattac tgtaaattca agtaattaat gcaacttttt caagagggaa taaaaggaaa
     6721 aaaaattatt ttcagggcaa aggaaaaaaa ctttttcccc tttgccctga tggaataaat
     6781 gttttacttc gattttttcc ctataaaaaa ttacaaaaat aaaacattaa ccatagcaca
     6841 aaaatacata atttaaacaa ggaaacacag aaatgcaaac aaaaagatat agcattagct
     6901 atcggaaaag ataaatcggt tgtatcatgt gaactgcgtc gaaactgtga taatcgaaac
     6961 gggtaacaca gagcggattt ggcacaacgc aaatgcgaaa ataggcagaa gaacaaacgt
     7021 aaatgtatac gttttacaga agaatgaata accccaaggc aagcctcgag gaattctttt
     7081 gaaataaaga aattccttaa aattgactat cctcgccgca agcagcgagg tattacgcca
     7141 attttatggc ttcgccacca gaatttctaa aaaaataaaa acaaaagcta ccccgacgca
     7201 agcatcgggg gattcttata attaagctaa ttatgctttg ccagcacttc ccaaaacgtt
     7261 ttgggtttta tgcatgtaaa gttttttcaa ttcatcacga gcagggccca aatatttacg
     7321 tggatcgaat tctgcaggtt gtgtccataa tattcttcgg atagctgcag tcatagctaa
     7381 acgaccatca gaatcaatat taatcttaca tactgccgat ttggctgctt cgcgaagttg
     7441 ttcttcagga acaccgatag aatcttttaa ttgaccacca taacgattaa tttcgtccac
     7501 atattcttgc ggtactgaag aagctccatg aagcacaata gggaatcctg gaatacgtct
     7561 ttcaatttct ttcaggatat caaaacgcaa tggtggtgga actaatctac cgtttgcatc
     7621 aactgtacat tgttcaggtt tgaatttata agctccatga gaagttccaa tggaaattgc
     7681 caaagaatct acacctgtac gacttacaaa atcaactact tgatctggat cagtataagt
     7741 atgatgttca gctttcacat cgtcttcaat accagctaac actcccaatt cgccctcaac
     7801 cgttacatca aactgatgag cataatcaac aacttttttt gtcaatgcta cattttcttc
     7861 gtaaggaaga tgagagccat caatcatgac agacgaaaat ccaaattcaa tacaactttt
     7921 acacagttca aagctatcgc catgatcaag atgaagcaca ataggaattg gataacccaa
     7981 ttcttttgca tattctactg caccttgagc catataacgc aacaaggtct gattggcata
     8041 tttgcgtgct ccacttgata cttgtaaaat cactggtgat tttgtttcaa cacaagcaga
     8101 aatgatggct tgcaattgtt ccatgttatt gaaattaaat gctggaattg catagcctcc
     8161 ttcaatagct tttttaaaca attctcttga atttacaaga cctaattctt tataacttac
     8221 cattttcttg aattttattg attaataatc ttattttaca aaagtagtat ttttctgtga
     8281 aatagaaaaa aacaaaagaa agcgttaaag aacattttaa taaaagataa aactttatca
     8341 tttaggttat tatgctaacc ttttcatagc ttcgatggta tcttctttgc tgccaaaagc
     8401 agaaaaacga atataacctt ctccactgat accgaaacct gcaccgggag tagtcacaat
     8461 atttttttct tttaacagaa aatcaaaata ttcccaactg ctcattccat tgggagtttt
     8521 tacccaaaca tatggagaat tgatacctcc ataaacagca aaaccatttt tggtcaatcc
     8581 ttctcgaatg attcgagcgt tttctaaata ataatcaata agagccttaa cctgttttct
     8641 tccatctgga gtaaacgttg cttctgctcc acgttgaaca atatacgatg tcccgttaaa
     8701 tttggtcgat tgtcttcgta gccataattt attaagcgac acggcttcac ctttttgagt
     8761 atatcccata acttgctggg gaataatggt ataaccgcaa cgtgtgcctg taaaaccagc
     8821 cgttttggaa aaacttctga actctatagc aacttcagtt gctccttcaa tctcataaat
     8881 actgtgtgga acattatttt cagtaataaa agcttcgtag gctgcatcaa aaaggataac
     8941 agcttgattt tctctggcat aatctaccca aactttcaat tgttctttcg ttaaagttgt
     9001 tccagtagga ttatttggat aacacaaata aattaaattt actttttctt ttggcaaagc
     9061 aggaacgaaa ttattctcct tattacaagg caaatacaca agattactcc actgattgtg
     9121 ctcatctggt tcgcctgctc gtcctgccat tgcattggta tcgacatata caggataagt
     9181 tggatcggtc acagccaccc gattattaat atgaaaaata tcgccaatat ttccggtatc
     9241 acttttggct ccatcagaaa taaatatttc atctacctca atttttattc ctcttttctg
     9301 aaaatcgtgt tgagcaatag cttctcttaa aaattcatat ccttgttcgg gtccataacc
     9361 tctgaaagtt tccttatgac gttgttcttc agcagctttt atcatagcat caactaccac
     9421 atcgggaaga ggaagggtaa catcgccaat tcccatccga attattttgg ctgatggatt
     9481 ttttgctttg tgttccgcta ctcgttttgc aatttctgaa aataaataat tttcagggat
     9541 tttgagaaaa ttttcattaa tgaatgccat atttttattt tgattgaaaa attttagaaa
     9601 tttttaataa taaataattg taaaacaaat aattacaaat caatttcacc ttcaaacacc
     9661 atttcagcag gacccgttaa gtaaacatga ttatcattat tattccattc tatttcaagt
     9721 tcaccaccta acaatttaac agtagtttta cgagaagata ttttattcag aacgcctgct
     9781 acaactactg cacaagctcc tgtgccacaa gccattgttt cgcctgaacc tcgctcccac
     9841 actcgcattt tcagaatatt ctctgaaatt ttttccacaa attcaacatt tgtccttttt
     9901 ggaaaaatgg aactgttttc aataatagga cctatcgttt taatatcaag ttgatcaatg
     9961 ttatctgtaa aaatcacggc atgcgggttt cccattgaaa cacaagtaat ggcataattt
    10021 attccattcc caaaatctac tggttcattc acaataggtg atttttgaaa aagaacagga
    10081 atttgttcag cctcaagaat tggttctccc atatccactg tcactttttc aactttctgt
    10141 ttgtcattta aaaataattt gagttttttg atacctgcca gcgtttccag cgttaattca
    10201 gttttgtctg ttaatccttt ttcataaaca tatttaccaa tacaccgggc agcgtttccg
    10261 cacatttcac cttctgaacc atcagcatta aacatttgca tctggaaatc tgccacagag
    10321 gaaggcaaaa ttaataccag accatcagct ccaatgcctt tgtgacgatc gctcaagcga
    10381 atagaaagtt cattgggatt tttaattttt tcttcaaagc cattaatata aatataatcg
    10441 ttaccgatgc cgtgcatttt agtaaacttc ataatcagta gcttttaaac atataaacaa
    10501 tttttccttt ttataaaaat tccttcacaa gaaggagaaa agatcaagat tgcaaaaata
    10561 ctcattaatt caaataattt tgtaaaattt ggaatgattt cagaaaacac aatattaaat
    10621 tgttgatttt aaagttttta agatttggtt tattcaacta attgccttat ggtttcaaca
    10681 aaatcatcaa tttccttttc tatcgtatca aaggaacaca tccagcgtaa ttcgtttgtt
    10741 tgttcattcc aggtgtaaaa tttgtatttc tcacgcaatg gttcaattat cttttcagga
    10801 ataattacaa atatcgaatt agtttgtacc ggttgtgtaa tttttatttc aggaatagtc
    10861 tgtaattttt ctgccatcat ttgtgccatt tggttggaat gcaatgccaa ctgcaaccat
    10921 aagttattgg ataagaacgc aataaattga gctgcgataa aacggttttt agaataaagt
    10981 tgcatggatt gcttctgata aaaagctgca ttttttctta attcactatt aaaaatcaaa
    11041 atggcttccc ccaacattaa accatttttt gtaccgccaa aactcataat atctacgcca
    11101 caatctactg tagcttcttt caaacctaca ttcaacgcag caacagcatt tgaaattctt
    11161 gctccatcaa tatggacata aagattgttt tcatgggcaa atttacataa tgcttttaat
    11221 tctgaaactg aatagattgt tcccaattct gtactttgac tgatagaaat cacttttggc
    11281 tgagaattat gaacatttcc aattcgatac aaaaaaggtt caatcatttc aggagttaat
    11341 ttgccatcgg gagttggaac ggttaaaaga gaacaaccga tattttgagc tactgctcca
    11401 cattcatcgg tattgatgtg agctgtttca gcacaaatga ctgcttgaaa aggtaaggta
    11461 caacatttca aactgacaat attagctcct gtgccattaa aagcatataa cacatcaaca
    11521 tcgccaaaaa tactttcaaa aactttttga gcctgcatcg tataactatc ttcaccatac
    11581 gaagtttcat gatcaacatt ggctttgata atggcattta agatttcagg atgaattcca
    11641 gaataattat cgctggcaaa acatttcata attaaataat ttattaaaag ttttgtaatt
    11701 aattcattta cacataatta tatgtattta attgccaaat aaatagcaac tactaataaa
    11761 aaaacaccat aaactttttt aaatatttca gatgagattc taatccctat ttttgatcct
    11821 aaaaaagcac caaccaccat tccaccagca ataagtgctg caaatagtaa attcaaatat
    11881 ccttctttgg cataaataat tacactgggt aaacctactg gtaactgtaa agatgccaat
    11941 gaagtggcaa ctgctttttt tgcatcatat tgaaaaaaat gaatcagcaa tggtacaatg
    12001 ataatacctc ctcctttccc aaaaagtccg gcaaaaattc cagcagctac gccaactaaa
    12061 atatacatcc agaaagattt agaaatgaca ggatcttctt ttacaataat ttcgtttttc
    12121 ttatgaaaat attttggtaa atcaaaatag ctaatggcta tacataataa gataaatgca
    12181 taaagtcgcg taagaaaagt ttcactgata ttgactgcaa tttcgcctcc aacaaaagag
    12241 cctaaaaaca atcccaatgc aatccataat gattttttga tattgatttg acctgctttg
    12301 taatattccc acacccctaa aatgccaaca ggcaacaaca tagcagtaag tgaagtggca
    12361 ttggcatgaa gaatgtctaa tccaaaaata accagcaaac acggcactaa tacaatacca
    12421 cctccaatac caaataaacc ggataaaatt ccagcaacaa ttcccagaat caataaaccc
    12481 gttaaataca tctcgatgat tttattttgc aaaagtacga ataaaatgat aattttttcg
    12541 acaaactatt ttttgaatag tgctaccaaa ttataactcg cttatttgaa aaataaaaat
    12601 ctgatttttt aataattcct ctattctgga tgggaaattt gtagggaaag ttagagttat
    12661 cttaggattg aatttctaat agcagatatt agaaaaaaat tttataatta gttgaatttc
    12721 aagataatac attcaaaaat atccgaagac aagaataaat ctcgtatttg aggctttgag
    12781 caaatggttt ggagagcata gggaatgtgg gggttgttat tatttattca tcgttaattc
    12841 attggcaatc atttcggctt gtttcaacac cgtttctgtt gcgagcattt gcatat
//
LOCUS       DAOSAS010000039        12462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3426_length_12462_cov_12.704522, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000039 DAOSAS010000000
VERSION     DAOSAS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12462
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3426_length_12462_cov_12.704522"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLR72_02940"
     CDS             <1..606
                     /locus_tag="PLR72_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOK36198.1"
                     /translation="EVLSVSEINAMVAAIDLSKPEGHRNKAIIEVLYGSGIRVSELIG
                     LQISKMNLKEGYMLVEGKGSKQRLVPLSPTSIEQIEMWKIDRNKLPIKKGNEDYLFLN
                     RRGTKMTRAMIFTIVKDLAAAAGIKKKVSPHTFRHSFATHLLENGANLRAIQQLLGHE
                     SITTTEIYTHIDIHYLRETILNFHPLNQHHPKMTNNAKNEE"
     gene            596..>1680
                     /locus_tag="PLR72_02945"
     CDS             596..>1680
                     /locus_tag="PLR72_02945"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOK36199.1"
                     /translation="MRNNKIFIPYFIVYLMLMFSFPFTSLSAQETLVVGQIVNQVNKQ
                     PIANAKIFLKNTSKIAVSNEEGYFLIRNSGKENVLEFSAMGYKNKEIKIKPGESIGIL
                     VEMEPEAVTINNSFTSKEKEAAIPIISQIRNHLEDNDETNSPDYKVIAIEQNLLILNK
                     IHKQNIDPQIYHYLQNASVTDSFLVVPMYMNEVKYQYTSKGKKEISKTNFSSPQTEEM
                     IISQVLGEINPSLNFYKPTVTFFGQKYISPLSEKAEFYYHFYLTDSILSDTGKLYEIH
                     FASKNQRHFAFEGKFKVDSATWAITYIEASTSKLDKSFIKNLTIFQKFEKNQENKWCR
                     SKEKILITFKDNNLVKKITSEFPLFFNR"
     assembly_gap    1681..1780
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1781..2808
                     /locus_tag="PLR72_02950"
     CDS             <1781..2808
                     /locus_tag="PLR72_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36200.1"
                     /translation="GGYLGYGFGNQEVKYSTFAQYRLPGETKRVLGINYTNDNRRIDY
                     NYNNFLYLENPLNSGDEDIASTIFSFRKAQRLNERKELTFSFLNDWNTNVESSIYFRS
                     IYQLSNEFLPFKINDVYCNSIQQNSITFSTRFSFDEKTFEDHLQRIYMYNHKPVIYMI
                     LEGGEYKVGNQKNNYGKLAGIVKQNTQFNWAQLNYWLEGGWLIGKVPYSLLAFQPGCE
                     GGYGFYRFSMMDYMQYAADKYFQFHGELIFNGRVWQKIPVIKYLTLKEFFTFKMTVGD
                     LGSSHQEVLDFPSYIIPLQEPYYEVGIGFANILHLFNVQSIWRFTENKDLVTPKWGIR
                     ASFRISF"
     gene            2911..3714
                     /gene="proC"
                     /locus_tag="PLR72_02955"
     CDS             2911..3714
                     /gene="proC"
                     /locus_tag="PLR72_02955"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOK36201.1"
                     /translation="MNLENLKIAVIGAGNMGSALLHGWIKSKSISPQNISISDISKEK
                     LEKITTFESGVNVSLSNKEVIKKAKIIILAVKPWLIEEIAEEIKNEINYKEQILISIA
                     AGINFEKLLKIFDRKAMLFRVVPNTAAEVSESLCAISSCNADSEQQQLIMNLFNCIGK
                     AFLVPENQLNAFMSLTSCGIAYVFRYIRAAMEGGVEIGISPEICQQAVVQTLKGAVAL
                     LEANQTHPEIEIDKVTTPGGITIKGLNEMEANGFSNAVIQGIKASWLKD"
     gene            3746..4294
                     /locus_tag="PLR72_02960"
     CDS             3746..4294
                     /locus_tag="PLR72_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOK36202.1"
                     /translation="MNEQIRQIALRLRGLRESLDLTISEAAEKCGVPKKDYEKYESGE
                     TDIPVSFICKVAETFGIETTALISGEEPRAQVFFVTRKGTGASIERTKAYKYQALAYG
                     FRNAKVDPFEVMVEPNNLPIYLNTHKNQEFNYVLEGTLLLEIDGKQLTLYEGDSIYFD
                     ATKPHGMKALNGKKVRFLAIII"
     gene            4418..6073
                     /locus_tag="PLR72_02965"
     CDS             4418..6073
                     /locus_tag="PLR72_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOK36203.1"
                     /translation="MLEKFVKQTEFKDFEDFNKNFQLIVPDHFNFAYDVVDEWAKTNP
                     NKQAMVWTNDQRECRKFTFADMKKYSDQTASYFQSLGIKKGDIVMLILKRRYEFWFSI
                     LALHKIGAIAIPATHLLTSKDLIYRNNAADIKAIVAVGEEPILTHINESLPDSTTVKF
                     LISIGPKVPENWLDFHKGIKEAAPFIPLYHINENEDPLIISFTSGTTGNPKMVLLDYR
                     YPLAHIITAKYWHNLHENSLHLTIADTGWLKALWGKIYGQWIAGACVFVYDHEKFTPS
                     AILSMIEKYKVTSLCAPPTIFRFLIREDLTKYDLSSLEYCTIAGEALNPSVYEKFLEL
                     TGIKLHEGYGQSETTVCIFTSPWTEPKPGSMGLPCAHYDIDLLTYDGRSAEVGEQGEI
                     VIRLDKNYPVGLFKGYYRNEDLTREAFRDNIYHTGDLAWRDEDGYFWFVGRTDDVIKS
                     SGYRIGPFEVESALMTHPAVVECAVTGIPDELRGQVVKATIVLSKEYKEKAGPALIKE
                     IQDHVKKVTAPYKYPRVIEFVDELPKTISGKIRRVEIREKDSQ"
     gene            6140..6793
                     /gene="upp"
                     /locus_tag="PLR72_02970"
     CDS             6140..6793
                     /gene="upp"
                     /locus_tag="PLR72_02970"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOK36204.1"
                     /translation="MQVVNFSDTNSILHHFLKEIRSVEIQKDSMRFRKNMERIGEIMA
                     YEISKTMSYVSETVQTPFGISPVNLINEKIVISTILRAGLPFYQGFLNFFDGAESAFA
                     SAYRKYKNAVDFDIYVEYIASPSIEGKTLILTDPMLATGSSMEMTYNTLLAKGKPAKI
                     YIAAVIASQPAINYVVSHFPEEKTSIWAAAIDPELNEHSYIIPGLGDAGDLAFGEKI"
     gene            complement(6906..7160)
                     /gene="rpsT"
                     /locus_tag="PLR72_02975"
     CDS             complement(6906..7160)
                     /gene="rpsT"
                     /locus_tag="PLR72_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOK36205.1"
                     /translation="MANHKSALKRIRQANKKRLHNRYYAKTARNAVKKLRTTTDKTTA
                     TEMLPKVTSMLDKLAKRNIIHKNKASNLKSKLTIYVNKLS"
     gene            complement(7193..7264)
                     /locus_tag="PLR72_02980"
     tRNA            complement(7193..7264)
                     /locus_tag="PLR72_02980"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7229..7231),aa:Glu,seq:ttc)
     gene            complement(7455..10535)
                     /gene="infB"
                     /locus_tag="PLR72_02985"
     CDS             complement(7455..10535)
                     /gene="infB"
                     /locus_tag="PLR72_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOK36206.1"
                     /translation="MPIRLSKACKDLNVGMNTAIDFLAKKGHKLTADPNVKLTDEMFL
                     LLAKEFNKDMALKIESERISQERQLKEKQETVALDDFIHHKPAEKEIETIPTTIPADQ
                     VPHLKSVGYIDLEEQIKKSKEKKEEVISQTSAKEKELTTKQKIQLKEEKLPETIIPEK
                     EKVTNIETTVSPKIELEEIKKEKTTVEKKEKILVAEVEEKEKTVEPKKISSKIKAESA
                     EKVEKQQISHEVEEKNLIGREEIFTAKIEETAENEEENEIFEIAHPILDTNLTVIGTI
                     DLDAINQSTRPKSKSKEQKKKEYAEKNRLLNESKKFAPSKSKKIIKLSEEETDEVELD
                     EFKEQHKKKRARFTKQKVDIAKIFPNGTSISTLEPPKKVVSPKVGKGKLKKTIKPAIS
                     EEEVQKQIKETLARLSAASSKKGKSAKYRRDKREMLNARQQELAQREQEEEKVLKLTE
                     FVTVNELANLMDVTVNDVIATCMNIGMIVSINQRLDAETINILADEFGYKVQYISQDI
                     LESVLEEEPDREEDLQPRPPIVTVMGHVDHGKTSLLDYIRKTNVIAGEAGGITQHIGA
                     YNVMLDNGQRITFLDTPGHEAFTAMRARGAQVTDIAIIIIAADDSVMPQTVEAINHAT
                     AANVPIVFAINKIDKPGVNPEKIKEELANMNYLVEEWGGKYQSQDISAKTGIGVPELL
                     EKVLLEAELLDLKANPKKRASGSVIESSLDKGRGYVATVLVQNGTLHQGDIVLAGIHY
                     GKVKAMFNERNQHITEAKPSEPALILGLNGAPQAGDNFIVMPSEHEAREIATKREQLQ
                     REQTIRTKKHFTLDDLGRRIALGNFKELNIIVKADVDGSVEALSDSLLKLSSDKIQVN
                     IIHKAVGAISDSDVLLAAASNAIICGFQVRPTSSARKLAEKEEIDIRLYSIIYDAIEE
                     IKSAMEGMLSPEIKEEVTATLEVRDVFKISKVGSVAGCFVREGKIKRTNKVHLIRDGI
                     VIYTGEIASLKRFKEDVKEVSANYECGISIQNFNDIHVGDIIESFEETEIKSTL"
     gene            complement(10606..11865)
                     /gene="nusA"
                     /locus_tag="PLR72_02990"
     CDS             complement(10606..11865)
                     /gene="nusA"
                     /locus_tag="PLR72_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOK36207.1"
                     /translation="MAKRETLNLIDSFSEFKDLKNIDRSTLISVVEESFRNIIAKKYG
                     TDENFDVIINPDKGDFEIYRNRTIVADENFQDPNLEIPLSEAQKIDEDYEIGEEVTDT
                     VDFMDFGRRAILTLKQTLSSKISDLHKENIYIKYKEKVGQIVTAELYQIWKNEMLLLD
                     EEGNELYLPKSEQIPTDFYRKGDMVRAVVAMVENRNNIPRITLSRVSPVFLERLFELE
                     IPEIHDGLITIKKIARIPGERAKVAVESYDDRIDPVGACVGIKGSRIHGIVRELRNEN
                     IDVINYTNNISLFIARSLSPAKISSIRINEEEKKAEIYLKPEEISQAIGKGGLNIKLA
                     SMLTGYTLDVFRELDEAETEDIYLDEFSDEIDEWVIDVLKSIGCDTAKSVLSISRDDL
                     IHRTDLEEETVDNVLAVLRAEFEEEEK"
     gene            complement(11870..12337)
                     /gene="rimP"
                     /locus_tag="PLR72_02995"
     CDS             complement(11870..12337)
                     /gene="rimP"
                     /locus_tag="PLR72_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOK36208.1"
                     /translation="MISEKTIHQLAEKFTENKDFYIVELKVNPDNQILIKFDSFNGVT
                     IDDCVSLSEFIESNLDREIEDYELEVSSPGLTEPFKVLQQYEKNIGNEVEVLTKGGNK
                     FSGKLLNATNNEFVVQIKKMKKLENSKRKTIVTENVIFHYDEIKTTKYILRFK"
BASE COUNT         3918 a         2045 c         2066 g         4333 t
ORIGIN      
        1 gaagtgcttt ccgtaagcga aatcaatgct atggttgctg ctattgactt aagtaaacct
       61 gaaggtcatc gaaacaaagc cattattgaa gttttgtatg gctcaggaat tcgcgtttca
      121 gaattaattg gtttacaaat ttcaaaaatg aatctcaaag aaggttatat gttggtggaa
      181 ggtaaaggca gtaaacaacg actggttcct ttgtcgccta cttccattga gcaaattgaa
      241 atgtggaaaa tagatcgaaa caaactgccc atcaaaaaag ggaatgaaga ttatcttttt
      301 ttgaacagac gaggcactaa aatgactcga gcaatgattt tcaccattgt taaagatttg
      361 gcagcagctg caggaataaa aaagaaggta agtccccaca cttttcggca ttcgtttgct
      421 acacatttgc tggaaaatgg agccaactta agggctattc aacagttgtt gggacatgaa
      481 tctattacca ctacagaaat ttatacccat attgacattc attatttgcg agaaactatt
      541 ttgaattttc atcctttgaa tcaacatcac cctaaaatga ccaacaatgc aaaaaatgag
      601 gaataataaa atattcatac cttattttat tgtttacctg atgttgatgt tctcattccc
      661 ttttacttct ttgtcagctc aagaaacact ggtggtcggc caaattgtta atcaagtgaa
      721 taagcaaccc attgcaaatg ctaagatttt tttaaagaac acttcaaaaa ttgctgtgag
      781 caatgaagaa gggtattttt taatcagaaa ttcaggcaaa gaaaacgtat tggaattttc
      841 agcaatgggt tataaaaaca aggaaataaa aattaagcct ggcgaaagca tagggatatt
      901 agtagaaatg gaaccagaag ctgttaccat taataattcc ttcactagta aagaaaaaga
      961 ggcagccata cctattataa gtcagattag aaaccatctt gaagataatg atgaaaccaa
     1021 ttcacctgac tataaagtta ttgctattga gcaaaatctt ttaattctaa ataaaattca
     1081 taaacaaaac attgacccac aaatttatca ttatttgcaa aatgcaagtg ttacagattc
     1141 ttttttagta gttcctatgt atatgaacga agtaaaatac caatatactt caaaaggaaa
     1201 gaaagaaatt tcaaaaacca atttttcttc tcctcaaact gaagaaatga taattagtca
     1261 agtattgggt gaaataaatc cttccttaaa tttttataag ccaacagtta ctttttttgg
     1321 acaaaaatac attagtcctt tatcagaaaa ggcagagttt tattatcatt tttatttaac
     1381 ggacagtatt ttaagtgata caggcaaact ttatgagatt cattttgctt caaaaaatca
     1441 aagacacttt gcttttgaag gcaagtttaa agttgattcg gccacatggg ctattactta
     1501 tatagaagct tctacttcaa aattagacaa aagttttatc aaaaacttaa cgatatttca
     1561 aaaatttgaa aaaaaccaag aaaataaatg gtgtcgctca aaggaaaaaa ttttaatcac
     1621 ttttaaggat aacaatttag taaaaaaaat tacaagtgaa tttcctttgt ttttcaatcg
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttggcggtta tttgggttat
     1801 ggttttggca atcaagaagt aaaatattct acttttgctc aatatcgttt gcctggcgag
     1861 acaaaacgtg tattgggtat aaattacacg aacgataatc gaagaattga ctacaactac
     1921 aataattttc tttatctgga aaatccttta aatagcggcg atgaagatat tgccagcacc
     1981 attttttcct ttagaaaggc tcaacgatta aatgaaagaa aagagttgac attttctttt
     2041 ttaaatgatt ggaatactaa tgtagaatcg agtatttatt ttcgttctat ttatcagttg
     2101 tcaaacgaat ttttaccttt caaaataaat gatgtttatt gtaatagtat tcaacaaaat
     2161 tcgattactt tttctacgcg tttttctttt gatgaaaaaa cgtttgaaga tcacctgcaa
     2221 cgcatttata tgtataatca taagcctgta atttatatga tattagaagg tggggaatat
     2281 aaagttggca atcaaaaaaa taattatgga aaattagcag gtattgtcaa acaaaatacc
     2341 caattcaact gggcacagtt gaattattgg ctggaaggtg gttggcttat tggaaaagtt
     2401 ccttattctt tgcttgcttt tcaaccgggt tgcgaaggtg gctatggttt ttatcgattt
     2461 agcatgatgg attatatgca atatgctgcc gataaatatt ttcaatttca tggtgaacta
     2521 attttcaatg gtcgagtatg gcaaaaaatt cctgtgatta aatatctcac gttaaaagaa
     2581 ttctttacct tcaaaatgac ggtaggcgat ttaggttcgt ctcatcaaga agtacttgat
     2641 ttcccttctt atattattcc tttacaggaa ccttattatg aagtaggaat tggttttgcc
     2701 aatattttgc atctattcaa tgtgcaatct atctggagat ttaccgaaaa taaagattta
     2761 gtaacaccta aatgggggat aagagccagt ttcagaatca gtttttgaat taattcggaa
     2821 aaaatggatg taaaatgaaa ataaaaatta taaataattt tatctttgta gtgactataa
     2881 gttattttta atcattaatc aaaatttttt atgaatctcg aaaacttaaa aatagccgta
     2941 attggtgcag gaaacatggg aagtgctttg ctacacggct ggataaaaag caaatcaata
     3001 tctcctcaaa atatcagtat aagtgatatt tcaaaagaaa aacttgaaaa aataacaact
     3061 tttgaatcag gtgtcaatgt tagtttatca aataaagaag tgataaaaaa agcaaaaatt
     3121 attattttag cagtaaaacc ttggttgata gaggaaattg cagaagaaat taaaaatgaa
     3181 attaactata aagaacaaat attaatttct attgctgcgg gaatcaattt tgaaaagtta
     3241 cttaaaattt ttgatagaaa ggcaatgtta tttcgggtgg ttcctaatac cgccgccgaa
     3301 gtttctgaga gtctttgtgc catttcctcg tgtaatgcag atagtgagca acaacaatta
     3361 ataatgaatc tttttaattg cataggtaaa gcttttttag tgccagaaaa tcaactgaat
     3421 gcttttatgt cgctcacttc gtgcggaata gcttatgttt ttcgatatat tcgcgcagcc
     3481 atggaaggcg gagtagaaat tggaatttct ccagaaattt gtcagcaagc tgttgttcaa
     3541 actttaaaag gtgctgtagc tttgcttgaa gccaatcaaa ctcatcccga aatagagatt
     3601 gataaagtga caacgccggg cggaattaca attaaaggtc ttaatgaaat ggaagctaat
     3661 ggtttttcta atgcagttat tcaaggaatc aaagcttctt ggttgaaaga ctaaagtcaa
     3721 taataacata taattttatc gcattatgaa tgaacaaatt agacaaatcg cgttgaggtt
     3781 acgtggctta cgtgaatctt tggatttgac aatttctgaa gcagcagaaa aatgtggagt
     3841 ccctaaaaaa gattatgaaa aatatgaatc gggcgaaaca gatataccag ttagcttcat
     3901 ttgtaaagta gctgaaactt ttggaataga aactaccgct ttaatttctg gagaggagcc
     3961 tcgtgcacaa gttttttttg tgactcgcaa aggaacagga gccagcattg aaaggacaaa
     4021 agcttataaa tatcaagctt tagcctatgg ttttcgaaat gcaaaagtag atccttttga
     4081 ggtaatggtt gagcctaata accttccaat ttatttgaat acacataaaa atcaggaatt
     4141 taattatgtt ttggaaggta ctttattact ggaaattgat ggaaaacaac taaccttata
     4201 tgaaggagat agtatttatt tcgatgctac caaaccacat ggaatgaaag cgttaaatgg
     4261 taaaaaagtg agatttttag ctataattat ttgaaacaat ttactgatga tattaaaaat
     4321 cataattttt gagatggaat aaaatttttt taaaaaagaa atgctttcgt aaataagaaa
     4381 gtgtttcttt ttttgttaaa ataaaaaaat tactatgatg ttagaaaaat ttgtaaaaca
     4441 aactgaattt aaagattttg aagattttaa taaaaatttt cagcttatag ttcctgacca
     4501 cttcaatttt gcttacgatg tagtagatga atgggcaaaa actaatccta acaaacaagc
     4561 tatggtatgg acaaatgatc aacgcgaatg cagaaaattc acttttgctg acatgaaaaa
     4621 atatagcgat caaacagctt catattttca aagtcttggg ataaaaaaag gagatattgt
     4681 gatgcttatt ttaaaacgac gttatgagtt ttggttttcc attttagccc ttcataaaat
     4741 tggtgcaata gccattcctg caactcattt actaacttcc aaagatttaa tttataggaa
     4801 caatgctgcc gatataaaag ctattgtagc tgtaggtgaa gaaccaattt taacacatat
     4861 caacgagtcg ttgcctgatt ctacaactgt aaaattttta atttcaattg gaccaaaagt
     4921 tcctgaaaac tggcttgatt ttcataaagg cattaaagaa gcagctcctt ttattccact
     4981 ttatcatatt aatgaaaatg aagacccatt aattattagt ttcacttcag gaaccacagg
     5041 aaatcctaag atggttttat tagattaccg ctatccattg gctcacatca ttacagcaaa
     5101 atactggcac aacttacacg aaaacagtct tcacttgacg attgctgata caggatggtt
     5161 aaaagctttg tggggaaaaa tttatggaca atggatcgca ggagcttgtg tttttgtgta
     5221 cgatcatgaa aaatttactc cttctgctat attgagtatg atcgaaaaat ataaagttac
     5281 ttcactttgt gcacctccta ctatttttcg ttttcttatt cgtgaagatc ttactaaata
     5341 cgatttatct tcactggaat attgtaccat tgcaggtgaa gctctcaatc cttctgttta
     5401 cgaaaaattt ttagaattaa ctggtattaa gcttcacgaa ggttatggac agagtgaaac
     5461 taccgtttgt atatttactt cgccgtggac agagcctaag cctggttcga tgggcttgcc
     5521 ttgtgcccat tatgatattg atttattgac ctatgacggt cgttcagccg aagttggtga
     5581 acaaggtgaa attgttatcc gtttggataa gaattatcca gtaggattgt tcaaaggtta
     5641 ttaccgaaat gaagatctga cgcgtgaagc ttttcgtgat aatatttatc atacaggtga
     5701 tttggcatgg cgtgatgaag atggttattt ctggttcgtt ggtcgcacag atgacgttat
     5761 caaaagttct ggctacagaa tcggaccatt tgaagtagaa agtgccttga tgacacatcc
     5821 tgctgtagta gaatgtgctg tgactggaat ccctgatgag cttcgtggac aagtagtaaa
     5881 agcaacgatt gtgctatcca aagaatataa agaaaaagca ggaccagctt tgataaaaga
     5941 aattcaggat catgtaaaaa aagtaacagc tccttataaa tatccccgtg ttattgaatt
     6001 tgtggatgaa ttacctaaaa ctattagtgg aaaaattcga cgcgttgaaa tcagagaaaa
     6061 agacagtcaa taaaaagaga ttatatatta actggaaatt gaatcttgtg ttttttaaat
     6121 atttgttaat caatatatta tgcaagttgt aaacttttcg gatacgaatt cgattcttca
     6181 tcattttttg aaagaaattc gttcagtaga gattcaaaaa gacagtatgc gttttagaaa
     6241 aaacatggaa agaattggtg aaataatggc ttacgagatt agtaaaacta tgtcttatgt
     6301 aagtgaaact gtccaaactc cttttggaat ttctcctgtt aatttgataa atgaaaaaat
     6361 tgtcatttcc actattttaa gagccggact acctttttat caaggatttc tgaatttttt
     6421 tgatggagca gaaagtgctt ttgcctctgc ttatcgcaaa tataagaacg ctgttgattt
     6481 tgatatttac gtagaatata ttgcctcacc ttctatagaa ggaaaaacac ttattctcac
     6541 tgatccaatg ctggcaacag gtagttcgat ggaaatgaca tacaatacgt tacttgccaa
     6601 aggaaaacca gctaaaattt atattgcagc cgtcattgca agtcagccag caataaatta
     6661 tgtcgtttct catttcccag aagaaaaaac cagcatttgg gcagcagcta ttgatcctga
     6721 gttgaacgaa cattcttata ttatacctgg tttgggcgat gcaggtgatt tagcctttgg
     6781 agagaaaata taattggaaa attattagga aaaaataaga gaagaaacct catttgaagt
     6841 aataaaaaaa ctctttatcc ttccaaaaaa gataaagagt tttttattaa atgaaacggt
     6901 cttttttacg ataacttatt aacataaata gtcagttttg acttcaaatt actggcttta
     6961 tttttatgaa taatatttct ttttgccagt ttgtcgagca tagaagtcac tttaggtagc
     7021 atttcagtag ctgttgtttt atcggttgtt gtacggagtt tttttacagc gttccttgcc
     7081 gtttttgcat aataacggtt atgcaacctt tttttgttgg cttgacgaat tcttttcaat
     7141 gctgatttat gatttgccat aattttttta aataatttgt ttatctattt tgtagcccat
     7201 agcagaattg aactgctctt tcaagaatga aaatcttgcg tcctaaccga tagacgaatg
     7261 ggccatcact tatattaaaa acaattccaa aagaggaatt gcggctgcaa agataatgta
     7321 gttttttgga ttacgaaagt ttttacttaa aaatcttttt attcctataa caatcaatgc
     7381 tttattaatt tgatgtgaaa acaagttgat atcttagctc atcttaataa attaatggag
     7441 agaggtaaaa actctcaaag tgtagattta atttctgttt cttcaaaact ttcaatgatg
     7501 tcacctacgt ggatgtcatt aaaattctga atgctgatac cgcattcgta atttgcactt
     7561 acttccttta cgtcctcttt gaaacgtttt aatgaagcaa tttccccagt gtagattaca
     7621 attccatcgc gaataagatg aactttatta gttcgtttga ttttaccttc tcgtacaaaa
     7681 caacctgcaa ctgatccaac ttttgaaatt ttaaatacat ctcgaacttc gagtgtagcg
     7741 gtcacctctt ccttaatttc aggggaaagc ataccttcca tagctgattt tatttcttct
     7801 atggcatcat aaataataga ataaagtcgg atgtcaattt cttctttttc tgccagttta
     7861 cgtgctgaag aagtaggacg tacctgaaat ccacaaataa tagcattaga ggcagcagca
     7921 agcaacacat ctgaatcaga aattgctcca acagccttat ggattatgtt tacctgaatt
     7981 ttatcgctcg ataatttaag caaagaatct gagagggctt ctactgaacc atctacatca
     8041 gctttcacaa taatattcag ttctttaaag ttgcccagtg caattcttct tcctaaatca
     8101 tccaatgtaa aatgtttttt agttcggatt gtttgttctc tttgcaactg ctcacgtttt
     8161 gtagcaattt cacgtgcttc atgttctgag ggcataacaa taaagttatc acctgcctga
     8221 ggtgcaccat ttaaccctaa aatcaaagct ggttcagaag gtttagcttc ggtaatgtgt
     8281 tgatttcgtt cattgaacat ggctttcact tttccataat gaataccagc caacacaata
     8341 tctccttgat gaagcgttcc attttgtacc aaaacagtgg caacataacc gcgacctttg
     8401 tcgagcgaag attcaatcac agaaccagaa gcacgttttt ttggatttgc ctttaaatca
     8461 agtaattcag cttccagcag aactttttcc agcaattcag gaacaccaat tcctgttttt
     8521 gctgaaatat cttgtgattg gtattttcct ccccattctt ctaccagata attcatattt
     8581 gccaattctt ctttaatctt ttctggatta actcccggtt tatcaatttt attaatagca
     8641 aaaacgatag ggacattagc agccgtagca tgattaatag cttcaaccgt ttgtggcatc
     8701 acgctatcat cagcagcaat aataattatg gcaatatcag taacttgtgc accgcgtgca
     8761 cgcatggctg taaaagcttc gtgtccaggc gtatcgagga aggtgattcg ttgtccgtta
     8821 tcgagcatca cattataagc accaatatgt tgggtgattc ctccggcttc accagcgatt
     8881 acattggttt tcctgatgta atcaagtaaa gaagtttttc cgtgatccac gtgtcccatg
     8941 accgtaacaa taggtggacg tggttgcaaa tcttcttccc tatctggttc ctcttcaaga
     9001 acagactcta atatatcctg actaatatat tgaactttat aaccaaattc atcagcaagt
     9061 atattaatgg tttctgcatc cagtcgttga ttaatggaaa caatcattcc aatgttcata
     9121 caggttgcga taacatcatt cacagtcaca tccatcaaat ttgccagttc gttaacagtg
     9181 acgaattccg ttaatttcaa aactttttct tcttcttgtt cccgttgagc aagttcctgt
     9241 tgacgggcat ttagcatttc acgtttatct cttcgatatt tggcactttt gccttttttg
     9301 ctggaagccg ctgaaagtct ggctaacgtc tccttgattt gcttctgaac ttcttcttcc
     9361 gaaatggcag gttttattgt ttttttaagt tttcccttgc caacttttgg tgaaaccact
     9421 tttttaggag gttctaatgt tgaaatagaa gtgccattgg ggaaaatttt agctatatct
     9481 actttttgtt ttgtaaaacg agcacgtttc tttttatgtt gttctttaaa ttcatccagt
     9541 tctacttcat cagtttcttc ttcggataac ttaattattt ttttactttt tgaaggagca
     9601 aactttttcg attcattcag caacctattt ttttcagcat attctttctt tttttgttcc
     9661 ttagattttg atttgggacg ggtactttga ttgatggcat ccagatcaat cgttcctatt
     9721 acggttaagt ttgtatctaa aataggatga gcaatttcga aaatttcatt ttcttcttca
     9781 ttctcagcag tttcttctat tttagcagtg aatatttctt ctcttccaat gagatttttt
     9841 tcttcaactt catgactgat ttgttgtttt tcaacttttt ctgcagattc agcttttatt
     9901 ttggaagata tttttttagg ctcaacagtt ttttcttttt cttccacttc ggctacaaga
     9961 attttctctt ttttttcaac agttgttttt tcttttttta tttcttctaa ttcaattttt
    10021 ggtgaaacag ttgtttcaat attagtaact ttttcttttt ccggaataat agtttccggt
    10081 agtttttctt ctttaagttg aattttttgt tttgttgtga gctctttttc tttagctgaa
    10141 gtttgtgaaa taacttcttc ctttttttct tttgattttt ttatttgttc ttccaaatca
    10201 atatagccta cggactttaa atgaggcact tgatcagcag gaatggttgt aggaatcgtt
    10261 tcaatttctt tttcggctgg tttatgatga ataaaatcat ccaaggcgac cgtttcttgt
    10321 ttttctttta actgtcgttc ctgactgatt cgttctgact cgattttcaa ggccatgtcc
    10381 ttgttaaatt ctttagcaag taaaagaaac atctcatccg tgagtttcac atttggatca
    10441 gctgtcaatt tatgaccttt ttttgctaaa aaatcaatgg cagtattcat gcctacattc
    10501 aaatctttac aagctttgct taatcttatg ggcatatttt atttctttgt ttttaaaatt
    10561 ttttataatg attctgccga attattttgt taaaaaatag aattcttatt tttcttcttc
    10621 ttcgaattca gctctcaata cagccagtac attatcaacc gtttcctctt ctaaatcagt
    10681 tctgtgaatc aaatcgtcac gtgaaataga caaaacactt ttagcagtat cgcagccaat
    10741 tgactttaaa acatcaatta cccactcgtc aatttcatca ctgaattcgt ccagataaat
    10801 gtcttctgtt tctgcctcat ccagttcacg gaaaacatcg agtgtataac ctgtcaacat
    10861 actagcaagt ttaatgttca atccaccttt accgattgcc tgtgaaattt cttctggctt
    10921 taaataaatt tcagcttttt tctcttcttc atttattctg atagaagata tttttgctgg
    10981 acttaatgat ctggcgataa ataagctgat gttgttcgta taatttatca catcaatatt
    11041 ttcgttccga agttcccgaa caatgccgtg aatacgcgat ccttttattc ctacacatgc
    11101 acccacaggg tcaattctat catcgtaaga ttctactgct acttttgctc gctcacctgg
    11161 gattctggca atttttttga tagtaatcaa tccatcgtga atttcaggaa tctctaattc
    11221 aaacaaacgt tctaaaaaaa cgggtgatac acgcgagagg gtgatacgag gaatattgtt
    11281 cctattttca accattgcca ccacggcacg taccatatct cccttgcggt aaaagtcagt
    11341 tggaatttgt tcggactttg gcaagtaaag ttcatttcct tcctcgtcga gaagtagcat
    11401 ttcatttttc cagatttgat aaagctctgc ggtgactatt tgtccaactt tctctttgta
    11461 tttgatataa atattctctt tgtgtaaatc agaaatttta gaggataatg tttgttttaa
    11521 tgttaaaata gctcttcgtc cgaaatccat aaaatcaaca gtatcggtga cttcttcacc
    11581 aatttcataa tcttcatcaa ttttttgagc ttcgctgagc ggaatttcaa gattcggatc
    11641 ctgaaaattt tcatcagcta caatagtacg atttcggtaa atttcaaaat cacctttatc
    11701 aggattaatg atcacatcaa aattttcgtc cgtaccgtat ttttttgcaa taatatttcg
    11761 gaacgattct tcaactacac tgattagtgt gcttctgtct atatttttca ggtctttgaa
    11821 ttctgaaaag ctatcaatta aattaagtgt ttctcttttg gccatggtgt tatttaaatc
    11881 ttaaaatata tttggttgtt tttatttcat cataatgaaa aataacattt tcagttacta
    11941 ttgtttttct tttggaattt tcgagttttt tcattttttt aatctgtaca acaaattcat
    12001 tattggttgc gttcaagagt tttcctgaaa atttatttcc tcctttagtc agtacttcta
    12061 cttcgttccc aatatttttt tcatattgtt gtaaaacctt aaatggctca gttaagccag
    12121 gtgaactgac ttctaattca taatcttcta tttcacggtc gagattcgat tcgatgaatt
    12181 cactgagact gacacaatca tcaatagtga caccattgaa agaatcgaac tttattaaga
    12241 tttgattatc tggattaact ttcaactcta ctatgtaaaa atccttattt tcagtaaatt
    12301 tttctgccag ttgatgaatg gttttttcgc ttatcatttc ctttcagcat attcatttaa
    12361 taaatgaggg gactttttaa gtcccctcca caattcaata attcgatgca aaaatacaaa
    12421 atattttatt taaatccaaa gaattatcag aatattacaa ag
//
LOCUS       DAOSAS010000040        12258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3491_length_12258_cov_13.751864, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000040 DAOSAS010000000
VERSION     DAOSAS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12258
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3491_length_12258_cov_13.751864"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..632
                     /gene="greA"
                     /locus_tag="PLR72_03000"
     CDS             159..632
                     /gene="greA"
                     /locus_tag="PLR72_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOK36209.1"
                     /translation="MGNITYMTSEGYKKLIEELNELENVQRPAISKQIAEARDKGDLS
                     ENAEYDAAKEAQGMLEMKIALLKETIASARLIDESRINTNEVQILTRVKIKNTKTQQV
                     MSYMIVSESEANLKEGKLSVTTPIAKGLLGKKVGDNVAIPVPNGIMQFEILEISL"
     gene            645..1040
                     /locus_tag="PLR72_03005"
     CDS             645..1040
                     /locus_tag="PLR72_03005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOK36210.1"
                     /translation="MATIFSKIASGEIPCYKVAEDENYFAFLDINPLTKGHTLVIPKK
                     EEDYLFQLDDPTYIGLMLFAKKIALAIQKAVPCKRIGMAVIGLEVPHAHIHLIPINNE
                     SDMNFKNPKLQLSSEEMTEIASAIRQFIQ"
     gene            complement(1093..3099)
                     /locus_tag="PLR72_03010"
     CDS             complement(1093..3099)
                     /locus_tag="PLR72_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOK36211.1"
                     /translation="MEILPLVKLDPWLEPYAEAIYGRYEYFLNTEKKLTEGKQSLSDF
                     ANGYLYFGLHHVPDGWVFREWAPNATSIYLIGDFNNWQRRPEYQLKKLQNGIWEIKLP
                     EYAMTHTQLYKLFVEWEGGSGERIPAWAQRVVQDPQTKIFSAQVWHPYQPYVFKIKNF
                     KPQKDPLLIYECHIGMSSQEEKISTYDEFRRNVLPRIAKLGYNAIQLMAIQEHPYYGS
                     FGYHVSSFFAASSRFGTPEELKQLIDEAHEMGIAVIMDLVHSHAVKNEVEGLGRFDGT
                     PYQYFHGGPRREHPAWDSLCLDYGKPEVMHFLLSNCKFWLDVYHFDGFRFDGVTSMIY
                     LSHGLGEDFTSYDCYYNLNQDGDAICYLTLANKLIHQVNPRAVTIAEEVSGMPGLAAK
                     IEDGGMGFDYRMAMNIPDFWIKIIKEVKDEDWKVGQIFWEVTNRRPEEKTISYVECHD
                     QALVGDKTIIFRLIDADMYWHMKRDDTTLQVDRGVALHKMIRLVTATTINGGYLNFMG
                     NEFGHPEWIDFPRPGNGWSFKYARRQWDLVDNQDYLYYNLNEFDRAMIALIKSVPAFN
                     KLPIQELWIKEDDQVLAYQRGKFVFVFNFNGTKSFTDYGILADKGAYKITLNTDNSLF
                     GGFNLVDESIVHFTIPEPPHFSGKEWLKLYIPARTAFVLKKQEM"
     gene            complement(3147..3593)
                     /locus_tag="PLR72_03015"
     CDS             complement(3147..3593)
                     /locus_tag="PLR72_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOK36212.1"
                     /translation="MILDNLENEKLYESVHPRFRKAFDFIKSTDLLRLPVGKIELEES
                     KLYVSIVEIEGKSEEEAKMETHKNFIDIQVPVDEKEIMGWKALQSLKKISQPYDSEKD
                     IAFYSDKASNLIKVQPGEFVIFFPTDGHQPGIFEGKHKKIIVKVLV"
     gene            complement(3628..4311)
                     /locus_tag="PLR72_03020"
     CDS             complement(3628..4311)
                     /locus_tag="PLR72_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOK36213.1"
                     /translation="MSIQPILDYLSELKNNNNRIWFNQNRDWYEEVRSEFLRISSALI
                     TEISKFDEEIKYVNLKDCLFRIYRDIRFSPDKSPYKTNFGVYIAAGGGRKSPRGGYYL
                     HLDPTGSFVSVGIWNPEPKVLKVLRKSVFENIDEFNEIRSNPEFSTYFSYFLEDGKLK
                     KIPAGFPKDFPYAEFLKLRHYIVDYKLDTSFFEDNHVVSRLAEIMKAGYPLNQFLNFT
                     VDELLLNSF"
     gene            4512..5531
                     /gene="pheS"
                     /locus_tag="PLR72_03025"
     CDS             4512..5531
                     /gene="pheS"
                     /locus_tag="PLR72_03025"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOK36214.1"
                     /translation="MKEKILQLQQEINQLTASSLEEVENLRIKYLSKKGKISLLFDEF
                     RNVPPAEKREIGQLLNQLKESAQSKINELKESLIISADEQQNIDLTRNADPIQIGTRH
                     PLSIIRNEIINIFGHIGFTIAEGPEIEDDWHVFESLNFPAEHPARDMQDTFFIEKNPD
                     VLLRTHTSSVQTRVMTQQKPPIRMLFPGRVYRNEAISARAHCFFHQVEGLYVDKNVSF
                     ADLKQALLYFAKEMFGMDTQVRLRPSYFPFTEPSAEMDISCNLCGGKGCAFCKYTGWV
                     EILGCGMVDPNVLENCGIDSKIYSGYAFGMGIERITNLKYQVKDLRMFSENDVRFLQQ
                     FTSAY"
     gene            5728..6546
                     /gene="nagB"
                     /locus_tag="PLR72_03030"
     CDS             5728..6546
                     /gene="nagB"
                     /locus_tag="PLR72_03030"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOK36215.1"
                     /translation="MRVIIQPDYQCLSRWAANYIAAKIIFSKPTKEKPFVLGLPTGSS
                     PLGMYHYLIELNKKGIISFENVITFNMDEYVGLPRDHEQSYYTFMWKNFFSHIDIKPE
                     NANILNGNAEDLQAECAAYEEKIKSVGGIDLFVGGIGPDGHIAFNEPGSSLSSRTRQK
                     TLTTDTIIANSRFFDNDITKVPKTALTVGVGTILDAKEVLILVNGYNKARALKHAVEG
                     AISQMWTVSALQLHPKAIFVCDYDACSELKVSTYKYFLDIEKDYLDPDSFNILI"
     gene            6564..7094
                     /locus_tag="PLR72_03035"
     CDS             6564..7094
                     /locus_tag="PLR72_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK36216.1"
                     /translation="MIYFHKIKTISNPYFNNFYQLYSSAFPENEQRSYSSLIKTLGSN
                     DHFSANAILNNNKFIGIFNYWKIEPFYFIEHFAVENNLRGQHYGTNTMKSFLSQTKLP
                     VILEVELPNTEVAIKRIQFYERLGFSLLPNDYFQPPYKKEALPIPLLLMSNDKLFASN
                     NFHFLKKVLHTEVYHF"
     gene            7272..8360
                     /locus_tag="PLR72_03040"
     CDS             7272..8360
                     /locus_tag="PLR72_03040"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOK36217.1"
                     /translation="MKKYFLPFIIMALLTVAFPISAQKASISFEEKVHDFGKIQEKDG
                     KVSYTFTFRNTGNSPLVISRVQASCGCTTPDWTKSPIEPGKTGSITITYNPLGRPGIF
                     NKTITVFSNAIEEQIQLIIKGEVIPEKKNDNEEYPISIEGIRLKNNIVQMNNITKGTI
                     QTREVPFLNSTSQNVKVSVEGLPNYITANVSPEIVPPQENGKILFSFDSRNCHQWGPI
                     SEDVYLVVNRQKKFSEDNKLLILANIVEDFSKLTVEEKQNAPIISISTLTLSFGNLKS
                     GDKKSGSFIIQNIGKNPLEIRRIINTNKELKIYPIKSVGGGKKAELKVEVDTKNLLPG
                     EYRKSFTLQTNDPDNSFTILTTTWTISK"
     gene            8430..9530
                     /gene="meaB"
                     /locus_tag="PLR72_03045"
     CDS             8430..9530
                     /gene="meaB"
                     /locus_tag="PLR72_03045"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOK36218.1"
                     /translation="MPKNSNKHPENDPLFKNLSVNEGVNKAPATNPYQQKKQKRKIYS
                     VPEYIHGILNRDISILSQAVTLIESSLPEHQKIAQEIIEKCLPYAGKSIRVGITGVPG
                     AGKSTFIETLGMYLIRSGHKLAVLAIDPSSERSKGSILGDKTRMEELSTSKNAFIRPS
                     PSAGTLGGVARKTRESIILCEAAGFDTIFVETVGVGQSETAVHSMVDFFLLIQIAGAG
                     DELQGIKRGIMEMADGIVINKCDGNNIDKAQIAKIQHQNALRLFPPTESGWSPEVLTC
                     SALERKGIDEVWNMIERYVQFTKANGYFEYKRNNQSKYWMYETIDEQLKVNFYQNEEI
                     QKKLIEVEKKVLANEISSFMGAKILLDLYFKK"
     gene            9573..10295
                     /locus_tag="PLR72_03050"
     CDS             9573..10295
                     /locus_tag="PLR72_03050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOK36219.1"
                     /translation="MLDINKIYNISCLDGLKQIPENTIDLIFTDPPYYQYRAQNIQGL
                     KNHKDLVTEFDFDHFDSEEDYLDFLTRVLSECYRVSKEGASGYLWCGDDFVSYFNRLV
                     EQVGFQFRKVIHWHKTNPFPAISSRKMFSNSMEMLVHFSKGTPKTWNYKSVNEMHNFI
                     EAPICMGKERTRHKTQKPLKVCIPFIEISSNVGDLILDPFIGSGTTAVACKMLKRNFI
                     GFEINEEFCKIAENRILHTKNE"
     gene            complement(10343..11344)
                     /locus_tag="PLR72_03055"
     CDS             complement(10343..11344)
                     /locus_tag="PLR72_03055"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOK36220.1"
                     /translation="MAKINAVITGVGGYVPDYILTNDELSTMMDTSDEWITSRVGIKE
                     RHVLKDKDLGTSFMAVKAIEDLFQKTGTKPEEIEMIICATATPDYILPSCASIIAEKV
                     GIKNAIAFDLEAACAGFIVALTTAAAFIESGRYKKILAIGAEKFTSLTNYEDRTTAPL
                     FGDAAGVVLVEPTEEEVGFIDSVLFTDGIGFSHLGTNGGISTHPPSHETVDKKMFTIY
                     QEGQYVFKHAVSDMSDAVVKIMERNHLTKDDVNWLIPHQANLRIIDAIAQRVGLDYEK
                     VIININKYGNTSAASIPLCLWEYENKFKKGDNIILTAFGAGFTWGAVYLKWGYSKQE"
     gene            complement(11428..11613)
                     /gene="rpmF"
                     /locus_tag="PLR72_03060"
     CDS             complement(11428..11613)
                     /gene="rpmF"
                     /locus_tag="PLR72_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOK36221.1"
                     /translation="MAHPKRKHSKQRTAKRRTHDKAKIPTLAICPNCGATYIYHTVCG
                     ECGYYRGKLAIEKSASV"
     gene            complement(11638..12204)
                     /locus_tag="PLR72_03065"
     CDS             complement(11638..12204)
                     /locus_tag="PLR72_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOK36222.1"
                     /translation="MSKFELYNVNLKDLSNEPQVYHFNLDDAYFQKIDSPEVQRGNIQ
                     ATVTVQKRSTLYHLDFVLDGYVVLQCTRCLEDMNQPIHDKENLDVKLGNKYSEENEVV
                     IVPESEGAINVAWFLYEFVVLNIPIKHVHPPGECNKTMINKLKIHIVHPAEEEDAEND
                     LFEMNDENDFLSEEPTTDFNWDLQNIDN"
BASE COUNT         4295 a         2075 c         1922 g         3966 t
ORIGIN      
        1 taattagaaa aatctttaaa agttttgtta atatcaaaaa tattaaatat atttgttttt
       61 taaataaatt tatcaaaagc actctctata taagagttcc agcaaagagt tggaattctt
      121 tttatttttt atatatcaaa aacctaaaat aagtaattat gggtaacata acttatatga
      181 cctccgaagg ttataaaaaa cttattgaag aattaaatga attagaaaat gttcaacgtc
      241 ctgctatttc caagcaaatt gctgaagcaa gggataaagg tgatttatct gaaaatgccg
      301 aatatgatgc agcaaaagag gctcagggaa tgcttgaaat gaaaatagct ttgttgaaag
      361 aaaccatagc atcagctcgt ttaattgatg aatcaagaat taataccaat gaagttcaga
      421 ttttgactcg cgttaaaatt aagaatacaa aaactcaaca ggtcatgtct tatatgattg
      481 tatcagagag tgaggcaaac ttaaaagaag gcaagttatc cgtaacaaca cctattgcaa
      541 aaggactgtt aggtaaaaaa gtgggtgata atgttgcaat tcctgttcct aatggaataa
      601 tgcaatttga aattcttgaa atttcacttt aaaaaataca aacgatggca acgatattca
      661 gtaaaattgc aagtggcgaa ataccttgtt ataaggtagc cgaagatgaa aattattttg
      721 cttttctgga tattaatcca cttacaaaag gtcatacatt ggttataccc aaaaaagagg
      781 aagattatct tttccagctt gacgacccga cttatattgg attaatgctt tttgcaaaga
      841 aaattgcttt agctattcaa aaagccgttc cttgtaaaag gataggcatg gctgtaattg
      901 gtttggaagt ccctcatgct catattcatc ttattcctat caataatgaa tcggatatga
      961 attttaaaaa tccaaagtta caactcagtt ctgaagaaat gacagaaatt gcgagtgcta
     1021 ttcgccaatt tattcaataa aaatgaaaag agtcgtattc aaaatttttt acgactcttt
     1081 aaaattacta aattacattt cttgcttttt cagcacaaaa gcagttcgag ctggaatgta
     1141 aagcttcaac cattcttttc cgctaaaatg tggtggctct ggaatggtaa aatgaacaat
     1201 actttcatct accagattaa atccaccaaa aagagaattg tctgtgttta aggttatttt
     1261 ataagcacct ttgtctgcta aaataccata atcagtaaaa gatttagtgc cattgaaatt
     1321 aaatacaaaa acaaatttac cgcgttggta agccaacact tgatcatctt ccttaatcca
     1381 caactcttga ataggtaatt tattgaaagc tggcacagat ttaataaggg caatcatagc
     1441 acgatcaaat tcattcaaat tataatataa ataatcctga ttatctacta aatcccattg
     1501 acgacgagca tacttgaatg accacccatt accagggcga ggaaagtcaa tccattcagg
     1561 atgtccaaac tcatttccca taaaattcaa atagccgcca ttgattgttg tggctgtgac
     1621 taaacgaatc attttgtgta aagccactcc acgatcaact tgtaaagtag tatcatcacg
     1681 tttcatgtgc caatacatat ctgcatcaat caatctgaaa ataatagttt tatcaccaac
     1741 cagtgcttga tcatgacatt ctacataact aatagttttt tcttctggtc tgcgattggt
     1801 tacttcccaa aaaatttgtc ctactttcca atcttcatct tttacttctt tgattatttt
     1861 tatccagaaa tcaggtatgt tcattgccat tcgataatcg aaacccatcc caccatcttc
     1921 aatttttgct gccaatccag gcatgccact cacctcttca gcgatggtca ctgcacgtgg
     1981 atttacttga tgaattagtt tattggcaag cgtaagatag caaatggcat caccatcctg
     2041 attcaaattg taataacaat cgtaacttgt gaaatcttct cctaaaccgt gacttagata
     2101 aatcatagaa gtgaccccat caaagcgaaa gccatcaaaa tgatatacat ctagccaaaa
     2161 tttacaattc gataaaagaa aatgcatcac ctccggtttt ccgtaatcaa gacacaacga
     2221 atcccacgca ggatgttctc tgcgaggtcc accatgaaaa tattgataag gcgttccgtc
     2281 aaaacgacct aaaccttcta cttcattttt aactgcatga gaatgaacca gatccatgat
     2341 tacggctatt cccatttcgt gagcttcgtc aatgagttgt tttaactctt cgggcgtacc
     2401 aaagcgagaa gaagcggcaa aaaaactgga tacatgatat ccaaaagagc cataataagg
     2461 gtgttcctga atagccatta attgaatggc attataacct aattttgcta tacgaggtaa
     2521 aacatttcgc ctaaattcat cgtaagtaga aattttttcc tcctggcttg acataccaat
     2581 atgacattca taaataagaa gaggatcttt ttgaggctta aaattcttaa tcttaaaaac
     2641 ataaggttga taaggatgcc acacttgagc actgaaaatt tttgtctgtg gatcttgtac
     2701 cactctttga gcccacgcag gaatacgttc accacttcca ccttcccatt ccacaaaaag
     2761 tttataaagt tgggtgtgag tcattgcata ttcgggtaat tttatttccc aaatgccatt
     2821 ttgtaacttt tttaactgat attcaggacg tcgctgccaa ttgttaaaat caccaataag
     2881 ataaatggac gtggcattgg gtgcccattc acgaaaaacc cagccatcag gaacatgatg
     2941 aagtccaaaa tacaaatatc catttgcaaa atcagataag ctttgttttc cttcagttaa
     3001 ttttttttct gtattcaaga aatattcata ccgtccataa atagcttctg cataaggttc
     3061 caaccaggga tccaacttaa caagaggtaa aatttccata tcttaaaatt cttgatctct
     3121 taacactaaa tttcctatca aaagttttaa accaaaacct taacgatgat tttcttgtgc
     3181 tttccttcaa aaataccagg ctgatgacca tctgttggga agaaaataac aaattcacct
     3241 ggttgcactt taattaaatt tgaagctttg tcgctgtaga aagcaatatc tttttcagaa
     3301 tcgtaaggct gagaaatttt tttcaaactt tgtaatgctt tccaacccat tatttctttc
     3361 tcatccacag gaacttgaat atcaatgaaa tttttatgcg tttccatttt tgcttcttct
     3421 tcacttttac cttctatttc tacaatgctt acatacaact tactctcctc caattcaatc
     3481 ttaccgacag gaagacggag taaatcagtg ctttttatga aatcaaaagc ttttctaaaa
     3541 cgaggatgta ctgactcgta caatttttca ttttctaaat tatctaaaat cataaataaa
     3601 agaattaaat gtttatttta atgacgttta aaaactattt aacaacagct catccactgt
     3661 aaaattcaag aattgattta aaggataacc agctttcatt atttcagcta agcgagacac
     3721 cacatgatta tcttcaaaaa atgaggtatc aagcttataa tcgactatgt aatgtcttaa
     3781 tttcaaaaat tcagcatacg gaaaatcttt agggaaacca gcaggaattt ttttaagttt
     3841 cccatcttcc aaaaaataag aaaagtaagt ggaaaattcg ggattggaac gaatttcatt
     3901 aaattcatca atgttttcaa aaacactttt acgcaatacc ttcaaaactt tgggctcagg
     3961 attccatata ccaaccgaaa caaaagaacc ggttggatca agatgtaaat agtagccacc
     4021 tcgaggactt ttgcgaccac ctcctgcagc aatataaact ccgaaatttg ttttataggg
     4081 acttttatcg ggagaaaaac ggatatcacg ataaattcta aaaagacaat cttttaaatt
     4141 aacgtactta atctcttcat caaacttgga aatttctgta ataagagcag aacttatcct
     4201 taaaaactca gacctaactt cttcatacca atctctgttt tgattaaacc atatacgatt
     4261 gttgttgttt ttcaattcac ttaaatagtc aagtataggt tgaatagaca tgttttcccc
     4321 ctttttaaaa acacaaagat actgaaaatt aaagaaaatt cattattttc cttaattttt
     4381 taaatttgtt attttcgaag aagataacgc tatttttcct gaaaataatt acctttgcaa
     4441 gttcaaatta gtaatgaatg attttaattt cttcaggaaa tttttatctg gtagaataaa
     4501 aggacattaa aatgaaagag aaaattttgc agttacaaca agaaattaat caattaactg
     4561 cctcatcgtt agaagaagta gaaaatttac ggattaaata tttgagtaaa aaaggaaaaa
     4621 tatccttgct ttttgatgaa ttcagaaatg ttcctccagc agaaaaacga gaaatcggac
     4681 aattactaaa ccaattaaaa gaaagtgctc aaagtaagat caatgagctg aaagaaagtt
     4741 taatcatttc agcagatgaa caacaaaata tcgacttaac aagaaatgcc gaccccattc
     4801 aaattgggac acgtcatcca ctttctatta tcagaaatga aattatcaat atttttggtc
     4861 acattggctt taccatagcc gaaggtcctg aaattgaaga cgactggcat gtttttgaat
     4921 cgctaaattt tcctgcagaa catcctgctc gcgacatgca ggatactttc ttcattgaaa
     4981 aaaatcccga tgttttgctg cgaacacaca cttcatcggt acaaacccgc gtaatgactc
     5041 agcaaaaacc acccatcagg atgttgttcc ctggtagagt ttatcgaaat gaagccatat
     5101 cagctcgggc tcattgcttt tttcatcaag tagaaggact gtatgtggat aaaaatgttt
     5161 cgtttgctga cctgaaacaa gctctcttgt actttgccaa agaaatgttt ggaatggata
     5221 cccaggtcag actcagacct tcttatttcc cttttacaga gcccagtgca gaaatggata
     5281 tttcctgcaa tctctgtgga ggaaaaggtt gtgctttttg taaatatacc ggttgggttg
     5341 aaattttagg ctgtggtatg gttgatccta atgtgctgga aaactgtgga atagattcaa
     5401 aaatttactc tggttatgct tttgggatgg gaattgaaag aattaccaat cttaaatatc
     5461 aagtaaaaga tttacgaatg ttttctgaaa atgacgttcg ttttcttcaa caatttactt
     5521 cagcatatta aagtaaaact gatttagtga taatattttt ttcaaaaagt tatttcagtt
     5581 tagctaaaaa gaggaattaa aaattaaaat aagagtaaac attgatggtt acccatggat
     5641 ttttaaaaat cattttttgt ttaaatgcac aatcattttt taaataatgg tatttttgta
     5701 aaaacaaatt ttattaaata aacgaatatg cgagtaatta ttcaacctga ttatcaatgt
     5761 ctttcaagat gggctgccaa ctatattgca gcaaaaatta ttttttcaaa accaaccaaa
     5821 gaaaaacctt ttgttttggg tttacctact ggctcttctc ctctgggaat gtatcactac
     5881 ttaattgaac taaataaaaa aggaattatt tcctttgaaa atgtcattac tttcaatatg
     5941 gatgaatatg tgggattgcc acgtgaccat gaacaaagtt attacacatt tatgtggaaa
     6001 aatttcttca gccatatcga tataaagcca gaaaatgcaa acatattgaa tggcaatgca
     6061 gaagatcttc aggcagaatg cgctgcttat gaagaaaaaa taaaatcggt tggaggaatt
     6121 gatttgtttg taggaggaat tggtcccgat ggacatattg cctttaatga accaggttct
     6181 tctttatctt ccagaactcg acaaaagaca ttaacaaccg ataccattat tgccaattca
     6241 cgtttttttg acaatgatat cacaaaagta ccaaaaacag ctctcacggt gggagttgga
     6301 accattcttg atgcaaaaga agtactgatt ctggtcaacg gttataacaa ggcaagagct
     6361 ttaaaacatg ctgtagaagg agcaatttcg cagatgtgga cagttagcgc tctccaatta
     6421 catccaaaag ccattttcgt ttgtgattat gatgcttgtt cagaattgaa agtcagcacc
     6481 tataaatatt ttctggatat tgaaaaagat tatctggatc ccgattcttt caatatcctt
     6541 atttaacgaa tcaaaaataa ggcatgattt attttcacaa aataaaaacc atttcaaatc
     6601 catattttaa taacttttat caattatatt cttctgcttt tcctgaaaat gaacaacgta
     6661 gttacagcag tttaatcaaa acattgggaa gtaatgacca tttctcagca aatgccatac
     6721 taaataataa caaatttatt ggcatattta actattggaa aattgagcca ttttacttta
     6781 tagaacattt tgccgttgaa aataatttgc gaggtcagca ttatggtact aacacgatga
     6841 aatcatttct ttctcaaaca aagttacctg ttattcttga agttgaattg cccaataccg
     6901 aagtagcaat aaaaagaatc cagttttatg aaaggcttgg tttttcttta ttaccaaatg
     6961 attattttca gcctccttac aaaaaagaag ctttacctat tcccctgctt cttatgtcaa
     7021 atgataaatt atttgcctcg aataattttc attttctaaa aaaggtactt catacagaag
     7081 tatatcattt ttgaacactt atcttagaaa tattttttcc catcattctt attttttcgt
     7141 gaaaaatttt ttcagattgt ttcacgattg atattttaac aaaattaaac agtaaacaat
     7201 gaaaaatttg acatatttag cattattttt ttttattttt gtaaagtcat atttaaaatc
     7261 aagtaattta tatgaaaaaa tattttttgc cttttataat aatggcttta ttaacagttg
     7321 cttttccaat atctgcacag aaagcaagca tttctttcga agaaaaagtt catgattttg
     7381 ggaaaattca agaaaaagat ggtaaagtaa gttatacttt cactttcaga aatactggga
     7441 attcacctct tgtgatttca agagttcagg cttcgtgtgg ttgtacaaca cctgattgga
     7501 ccaaatcacc tattgaacca ggtaaaactg gctctattac aattacttac aatccgcttg
     7561 ggcgacctgg tatttttaac aaaactatta cggtttttag caatgccatc gaagaacaaa
     7621 ttcaattgat tatcaaagga gaagtaattc cagaaaagaa aaatgataac gaggaatacc
     7681 caatttcgat tgaaggaatt cgtttaaaaa acaatattgt tcaaatgaac aatattacca
     7741 aaggtactat tcaaaccaga gaagttcctt ttttaaattc taccagtcaa aatgtaaaag
     7801 taagtgttga gggattacca aattatatta cagccaatgt aagcccagaa attgtacctc
     7861 cacaagaaaa tggaaaaatt cttttttctt tcgattcaag aaactgtcat caatggggac
     7921 caatttctga agatgtttat ttagtggtta atcgacaaaa aaagttttct gaagacaata
     7981 aacttcttat tttggccaac attgttgaag actttagtaa acttaccgtt gaagaaaaac
     8041 aaaatgctcc cattatatct atttcaacct taacactttc atttggtaat ttaaaatctg
     8101 gagataaaaa atcaggaagt ttcattatac aaaatattgg aaaaaatcca ctggaaatca
     8161 gaagaatcat aaacaccaac aaagagttaa aaatatatcc aataaaatca gtaggtggtg
     8221 ggaaaaaagc tgaattgaaa gtagaggtag ataccaaaaa tctacttcca ggcgagtata
     8281 gaaaaagttt tactctccag acaaatgacc ctgataatag ctttacaatt ttaactacaa
     8341 cttggactat ttcaaaataa aaaaatcttt ttgttaaatc gaatcgaaat tatgagttgt
     8401 ggtttgttta gtgtgaaaac tatttgaata tgcctaaaaa ttccaataaa catccagaaa
     8461 acgatccttt atttaaaaat ttaagtgtaa atgaaggagt taataaagca cctgcgacca
     8521 atccttacca acaaaaaaaa caaaaaagaa aaatttattc tgttcctgaa tatattcacg
     8581 gaattttaaa tagagatatt tctattttaa gtcaggcagt tactctgata gaaagttcat
     8641 tgccagaaca tcaaaaaatt gctcaagaaa taattgaaaa atgtttacca tatgctggca
     8701 aatcaattcg agtaggaatc acaggggttc caggagcagg taaaagtact tttattgaaa
     8761 cattgggaat gtatttgata cgttcaggac ataaattggc ggttctggca atagatccca
     8821 gcagtgaaag aagcaaagga agcatattgg gagataaaac gcgaatggaa gaattgtcta
     8881 cttcaaagaa tgcttttatt cgtccttcgc catcagcagg gacattagga ggagttgcaa
     8941 gaaaaacgcg cgaaagcatt attctttgtg aagctgctgg tttcgatact atttttgtag
     9001 aaacagtagg tgtaggtcaa tctgagacag ctgttcattc tatggttgac ttttttttat
     9061 taatacagat tgcaggagct ggtgatgaat tgcaaggaat aaaaagaggc attatggaaa
     9121 tggccgatgg tatcgtgata aataaatgtg acgggaacaa cattgataaa gctcaaattg
     9181 ctaaaattca gcatcaaaac gctcttcgct tatttcctcc aacagaatct ggctggtcac
     9241 ctgaagtgct tacttgttcg gcactcgaac gaaaaggtat agatgaagtg tggaatatga
     9301 ttgaaagata tgttcaattt actaaagcaa atggctattt tgaatataaa cgaaacaacc
     9361 aatcaaaata ctggatgtat gaaactattg atgaacaact taaagttaat ttttaccaaa
     9421 atgaagaaat tcagaaaaaa ttaattgaag tggagaaaaa agtacttgcc aacgaaatca
     9481 gttcctttat gggagcaaaa atattgctcg atttgtattt caaaaaataa agattttata
     9541 tcaaaaataa aaactccaat tacctacata ttttgcttga tatcaataaa atatataata
     9601 tttcctgtct ggatggctta aaacaaattc cagaaaatac cattgattta atttttactg
     9661 atccccctta ctatcaatat cgtgctcaaa atatacaagg gcttaaaaat cataaagatt
     9721 tagttactga atttgatttt gatcattttg attcagaaga agactatttg gatttcttga
     9781 cgagggtctt atcagaatgt tatcgagtaa gcaaagaagg agcaagtggt tatttgtggt
     9841 gtggtgatga ttttgtttcc tatttcaatc gtctggtgga acaagttggg tttcaattca
     9901 ggaaagttat acactggcat aaaacaaacc ctttccctgc tatttcaagc cgtaaaatgt
     9961 tttcaaacag catggaaatg cttgtacatt tttcaaaagg tactccaaaa acctggaact
    10021 ataaatcagt aaatgaaatg cataatttta ttgaagcacc tatctgtatg gggaaagaac
    10081 gtaccaggca taagactcaa aaacctttaa aagtgtgcat tccatttatt gaaataagca
    10141 gtaatgtagg tgatttgatt ctcgatcctt ttattggttc aggaacaaca gcagttgctt
    10201 gcaaaatgtt aaaaaggaac tttattggat ttgaaataaa tgaagaattt tgcaaaattg
    10261 cagaaaatcg gattttacat acaaaaaatg aataaaaaaa gaataccctt tttggatatt
    10321 ctttttaaaa aagctttgtt tattattctt gtttactata gccccatttt aaatacactg
    10381 caccccatgt aaagcctgca ccaaaagcgg taagaataat attatctcct tttttaaatt
    10441 tattttcata ttcccaaaga cataacggaa tactagccgc tgaagtatta ccatatttat
    10501 taatattgat aataactttc tcataatcta aacctactcg ctgagcaata gcatcaataa
    10561 ttcgtaagtt tgcttgatga gggattaacc aattaacatc gtcttttgta agatgatttc
    10621 tttccattat ttttactacg gcgtcagaca tgtcggaaac tgcatgttta aatacatatt
    10681 gaccttcttg ataaatggta aacatttttt tatctactgt ttcatgagaa gggggatgag
    10741 ttgatatgcc accatttgtg cctaaatgag aaaaaccaat tccatcagta aaaagaacag
    10801 agtcaataaa acctacttcc tcttctgtcg gttctaccag gactaccccg gcagcatcac
    10861 caaaaagagg cgcagtggta cgatcttcat aatttgtaag agaagtaaat ttttctgctc
    10921 ctattgccag aattttttta tatcttcctg attcaataaa agcagctgcg gtagtaagag
    10981 ctacaatgaa tcctgcacag gcagcctcta aatcaaaagc tatggcattt tttattccaa
    11041 ctttttcagc gataattgag gcacacgaag gtaaaatata atcaggagtt gctgtagcac
    11101 atattatcat ttcaatttct tcaggttttg tgcctgtttt ttgaaataaa tcttcaatgg
    11161 cttttactgc cataaatgaa gttcccaaat ccttatcttt taaaacatga cgttctttaa
    11221 ttcccacacg actggtaatc cattcatcac ttgtgtccat catggtactt aattcatcat
    11281 tggtaaggat ataatctgga acatatcctc caactcctgt gattacagca ttaattttag
    11341 ccatttttta atttagatat aaaaagtagc ctcagaagta attgttactg agagcaaaat
    11401 aatttattaa ttttaaaata aagattttca aacggatgca gatttctcta tagctaattt
    11461 tcctcgataa taaccacatt cgccacaaac agtatgataa atataagtgg cgccacaatt
    11521 tggacaaata gctaatgttg gaatttttgc tttatcatgg gtccttctct tagcagtacg
    11581 ttgttttgaa tgttttcttt ttggatgtgc cattttttct attatttatt tatttattca
    11641 attatcaatg ttttgtaaat cccaattaaa atctgtagtt ggttcctccg ataagaaatc
    11701 attctcatca ttcatctcaa ataagtcatt ctcagcatct tcttcttcgg caggatgaac
    11761 tatatgaatt tttagtttat ttatcattgt cttgttacat tcacctggcg gatgaacatg
    11821 cttaatggga atattcaata ccacaaattc atataaaaac catgctacat taattgcacc
    11881 ttctgattca ggcacaataa ctacttcgtt ttcttcagaa tatttatttc ctaactttac
    11941 atctaaattt tccttgtcat gaataggttg attcatatcc tccaagcaac gtgtgcattg
    12001 caacacaaca taaccatcaa gaacaaaatc aagatgataa agtgttgatc ttttctgaac
    12061 agtaactgtt gcttgaatat tgcctctttg aacttcagga ctatcaattt tttgaaaata
    12121 agcatcgtct aaattaaaat gataaacttg aggttcattg gataaatcct ttaaattgac
    12181 attatataat tcaaattttg acatggctta ttagcaatta aaaaaacggt acaaaggtat
    12241 aaaaatctct gcaattgt
//
LOCUS       DAOSAS010000041        12137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3524_length_12137_cov_14.555454, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000041 DAOSAS010000000
VERSION     DAOSAS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12137
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3524_length_12137_cov_14.555454"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..457
                     /locus_tag="PLR72_03070"
     CDS             158..457
                     /locus_tag="PLR72_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK36223.1"
                     /translation="MGAFDNSGRPTVALDAFKEAAASVISPKNKPKWIFKPNTHTLLF
                     ENKQERISIFQMNGRLVNSFFATNTISLNDLQNGIYIVKAVSYKNEKPEIFKLVK"
     gene            581..1894
                     /locus_tag="PLR72_03075"
     CDS             581..1894
                     /locus_tag="PLR72_03075"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOK36224.1"
                     /translation="MKVLKFGGTSVGSAERMKKVAELISNDEQKIVVLSAMSGTTNSL
                     LEIADYFYKNNVDGALEHIIALENKYFDVIEELYQTEKKKSEAKDILKSHFDYLRSFA
                     NAVFTLFEEKAVVAQGELLSTNLFQLYLEEIGENSILLPALDFMRLDKNGEPDTEYIK
                     MHLLELLKANPGFKIYLTQGFICRNCYGEIDNLQRGGSDYTASLIGAAVEAEEIQIWT
                     DIDGLHNNDPRYVKNTKAIPMLNFEEAAELAYFGAKILHPTCILPAKLANIPVRLLNT
                     LQPEAPGTLISTQINKGKIEAVAAKDGITAIKIKSGRMLLAYGFLRKVFEIFESYKTP
                     IDMITTSEVGVSVTIDNTKYLEEIVNDLKKLGTVSVDKDMVIVCVVGDLFYENVGFQA
                     QIVEALKDIPIRMISYGGSNYNISFLIKKEDKGKALNALSDALFN"
     gene            1946..3115
                     /gene="lysA"
                     /locus_tag="PLR72_03080"
     CDS             1946..3115
                     /gene="lysA"
                     /locus_tag="PLR72_03080"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOK36225.1"
                     /translation="MKAQYPIEKFSKLQTPFYYYDLDILRKTLQEVNKQIQKSDYIVH
                     YAIKANANPALLHEIKDTGFGADCVSGGEINAAIKAGIPASNIVFAGVGKTDWEINLA
                     LDKDIFCFNVESIPELEVINQLAKAKGKITNIALRLNPNINPHTHHYITTGLEENKFG
                     INLSDLDEVMNLLPKLKNLKLIGVHAHIGSQITDMSSFKSLCIRINEIQQYFFARRII
                     LEHVNVGGGLGVNYEHPNHFPIPDFESYFNVFRQHLLLQPQQILHFELGRSIVAHCGS
                     LISRVTFVKKGATKKFAILDAGFTDLIRPALYQAYHRIENLTSDLPEEEYDVVGPICE
                     SSDVFAKNYPMNQARRGDLIALRSAGAYGESMASQYNLRPLPKTYTSDQLLLEDN"
     gene            3173..4333
                     /locus_tag="PLR72_03085"
     CDS             3173..4333
                     /locus_tag="PLR72_03085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK36226.1"
                     /translation="MEIFNIYFSRAELYFLGGLLLTFIYQIYFYFHYINGVIHYRKKI
                     QHQKVSFLEKQPPVSVIICAKNEAENLSKFLPAILEQDYPDFEVIVINDGSVDDTDIL
                     LGAFSKKYPNLRTTFVPQEAQNLSTKKLGITLGVKAAKNDLLLFTDADCVPASHQWIK
                     LMVRNFLPDIEFVLGYSGYSSQKGFLNQLITYDTLFIGLQYMGMALAGKPYMGVGRNL
                     AYRKQTFFDQNGFASSLNLLAGDDDLMVNRAANKKNTRVEISPESITWSRPKTTFKSW
                     YQQKKRHLSVSSAYTKKSKWRLMLEPLTRGLFYILLIVTCIVGNNITKIFAVFLFLVR
                     YAIQITIINRSARHFKERQYFLTLLVFDLLLPLIPLSILATNRKLSRYNKKW"
     gene            complement(4342..4896)
                     /gene="rsmD"
                     /locus_tag="PLR72_03090"
     CDS             complement(4342..4896)
                     /gene="rsmD"
                     /locus_tag="PLR72_03090"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018910058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOK36227.1"
                     /translation="MRIISGKYKGRKIYAPINISARPTTDFAKEGLFDLLINRIDLEG
                     IDVLDLFAGTGGIGFEFVSRGCNKVVSIETDKNQCAFIRKVVSDLKIDNLFLFRTDVF
                     KYIASTHAQYDMIFADPPYLLKNIAEIPDLIFSYHLLKPNGIFVLEHSAKYNFQHHPH
                     FVEQRSYGKVNFTFFKVNPSKEIF"
     gene            complement(4899..5699)
                     /locus_tag="PLR72_03095"
     CDS             complement(4899..5699)
                     /locus_tag="PLR72_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOK36228.1"
                     /translation="MLGTGNNFQLQTCNMLSENISSQEKFFKESNLYNLSIRQKPNGF
                     SFFVYDDQKRLISLKNIKAPLFVMNKEELIPLLLKEPEMQLPYHNVKLTYESNRYSVV
                     PQMIFKKVEAESLFYFHHKKEKNEIVLSNPIASFETVNIFSVPSIIYEVFEQFFPKLN
                     IEHHITQFLINLQAYTNKEENLNVYLREHLMDVVAIENNQLMLINSFVFQTPEDFVYY
                     LLRVIEELSFDIDGCKIRLFNYEKRHSKLYNLAQKYMKHCEIIEKKRE"
     gene            complement(5680..6360)
                     /locus_tag="PLR72_03100"
     CDS             complement(5680..6360)
                     /locus_tag="PLR72_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36229.1"
                     /translation="MKTTIKVLLSLAAIFLAYLCVMSILTPINFERIRAQREKDVIQR
                     LIEIRKAEVEFKEQNGRYTASFDTLINFLKTAKKKIVLKEGFLTDEQLEAGLTEEKAA
                     QIVRRGNKQEIEKYGLQNFRRDTIKVDLLEEVFPNYTRQTIDQICYIPYSQGEKFELR
                     VNNDYVNKSGIRIPLFEACAPYETYLHDLNRQELLNLIDRAKKLNKYPGLKVGSIVEP
                     NNNAGNWE"
     gene            6650..8887
                     /locus_tag="PLR72_03105"
     CDS             6650..8887
                     /locus_tag="PLR72_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOK36230.1"
                     /translation="MTSYSTLPVSVLYGKVLQASENLFSFTERKEIKNILKQYCKSHP
                     LLFVKNNNSIEDKLSVIQILLTEIGLGKEMVISILFFDVLKEGLISISEIEQKFHIKI
                     SPIIEGLEKVNELYSKKTTLETENFSQFLLSVAKDVRVVLILIVQKLYTLRTLEKFSV
                     EKQQEIAQECAVLYAPLAHRLGLYKIKSELEDLALKYTNREVYKEIANKLNETKRSRD
                     QYIAEFIAPLEEKLRQEIEYPFEIKGRTKSIFSIYNKIKKQNTTFENIYDLFAIRIIL
                     DVPLEKEKMICWQVYSIVTDMYQPNPKRLRDWISIPKSNGYESLHITVLGPQNKWIEI
                     QIRTTRMNEIAEKGLAAHWKYKGIQSESEMDKWLKNIRDILENPQLNAIDLLDEFKLN
                     LYDKEVFVFTPTGELLRFPKGATVLDFAFYIHTNLGLKCTGALITNNTYQEKNVPLKY
                     VLKNGDQVKILTSATQKPSAEWLNIVATSKAKAKIRQALKEIQYKDAVIGRETLLRRF
                     KNWKIELDEGKISRLAKKKGYKTVSDFYQEIAQEKLDLLALRDTYLEMEKHEAQQNVT
                     ETRSAENFIKEPPVIETSIKEKEDILIIDQNLKGIDYTLAKCCQPIYGDEIFGFVSVG
                     GGIKIHRVDCPNAPELYSKFGYRIVKAQWSGQSGGTQYPITLHVIGKDDIGIVTNITS
                     LIDKEKNIVLRSISIDSKAGLFIGNLTIMVNDAHDLDGIMKKISMIKGIVNVSRIKTI
                     NKYNS"
     gene            8900..9505
                     /locus_tag="PLR72_03110"
     CDS             8900..9505
                     /locus_tag="PLR72_03110"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOK36231.1"
                     /translation="MFSGIIEEAAPVVALKKEEGNLHITMSCSFVNELTIDQSIAHNG
                     VCLTVVSKTDDSYTVTAIQETLDRTNLGELVCGNEVNLERSLLLTERLDGHIVQGHVD
                     TTAVCTAIDEVEGSWYFTFEYQFDPEMAKRGYLTVDKGSVAVNGVSLTVCNPTDNSFQ
                     VAIIPYTYEHTNFHQIKVGTTVNIEFDIIGKYISRLMKYNV"
     gene            9587..10723
                     /locus_tag="PLR72_03115"
     CDS             9587..10723
                     /locus_tag="PLR72_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOK36232.1"
                     /translation="MDKLRKQIKESFFNPIYHFFPLIVFLIVDNVAGRNWAWSIAIPL
                     FLILTIYLFIAYNQLFVWYFFSAFIFLAVNLSAIFTFLSEKWLFLSPIIDDLVCWLFL
                     GLTLLFRSFIEKKINARTPKFLPMKNNFEEYFNFIQSLFLIISGYLLFYFIIYQFPTI
                     KENIYLELLQVIYIGAIVFLLVFEFCRVQIVRHKLLKEEWWPIVNEQGKIVGNVQPLV
                     SLKIEKKYMHPIIRILFIKENMIFLQKNCENDLIFPCLWDAAISNHIRTGETIEDCIE
                     RTAEEKYGLKDLKPFFLSNYIHRTENEQHYAFLFIACNVNNIEPNYQKINQAKWWTLQ
                     QIEENLSTGIFTQNFIIEYNLLQRSGVFENKICNCECKLKELFS"
     gene            10821..12062
                     /gene="pepT"
                     /locus_tag="PLR72_03120"
     CDS             10821..12062
                     /gene="pepT"
                     /locus_tag="PLR72_03120"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOK36233.1"
                     /translation="MTTLVDRFLKYVEFTTTSDENTNLTPSTPGQLALAHYLVKELKS
                     IGLKDAEVDKNGYVMATLPANIDKEIPTIGFIAHLDTSPDMSGKNVKPKIIKNYDGKD
                     ILLNEEKLIVFETEKFPEILQYIGQDIIVTDGTTLLGADDKAGIAEIISAMEYLVDHP
                     EIKHGKIRIAFTPDEEIGKGVDYFNVKKFNAQWAYTIDGGEIGELQYENFNAASAKLT
                     FNGLNVHPGYARHKMKNSIRIAQQYMSMLPRHETPEHTEGYEGFYHITNIEGTVEKTT
                     LNCLIRDHDRDKFERRKKELQHLVNKINAEFGENTAIIELKDQYYNMREKIEPVMYII
                     DIASQAMKEVGVTPKISPIRGGTDGARLSYMELPCPNIFAGGHNFHGRFEYIPIPSME
                     KAMMVIVKIAELVDNQENISQ"
BASE COUNT         4252 a         1919 c         1969 g         3997 t
ORIGIN      
        1 ttaaaaatta aaactgacaa attctgattt ttgtcggtta atagtgaaaa aatttctttc
       61 cgatctgata acaaaaacaa aaagcgtttc taatggaaaa ggattaggag tgttttattg
      121 agaaccagaa tgttacaata attggaatgg ttatacaatg ggggcatttg acaatagtgg
      181 aagacccacc gtagcacttg atgcttttaa agaagctgct gcttcagtca tttcacccaa
      241 aaataaacca aaatggattt tcaagccaaa tactcatact cttttgtttg aaaataaaca
      301 agaaagaatt tctatttttc aaatgaatgg aagattggta aactcatttt ttgctactaa
      361 tacaatttcg ttgaatgacc tgcaaaacgg catttatatt gtgaaagctg ttagctataa
      421 aaatgaaaaa cctgaaattt tcaaactggt aaaataataa aaatttgcta ctcattaaat
      481 caaagaaaat tactaccttt gcaagtaatt ttaacattct tgcaattatt tttaattgaa
      541 ttataactta ttgatacata atttttaaac aaattatcaa atgaaagttt taaaatttgg
      601 tggcacttct gtgggctcgg ctgaacgaat gaaaaaagtg gcagagctta tttcaaatga
      661 cgaacagaaa attgttgtat tatcagccat gtcaggaact actaacagcc tgctggaaat
      721 tgcagattat ttttacaaaa ataatgtgga tggagcattg gaacatatta ttgctctgga
      781 aaataaatat tttgatgtca ttgaagaatt atatcaaacc gaaaagaaaa aatcagaagc
      841 aaaagatatt ctcaaatctc attttgatta tttgcgttca tttgctaatg ccgtttttac
      901 tttatttgaa gaaaaggctg tagtagcaca aggtgaatta ctttctacca acttatttca
      961 actttatctg gaagaaattg gagaaaatag cattcttttg cccgctctgg attttatgcg
     1021 attggacaaa aatggtgaac ctgataccga atacatcaaa atgcacttat tggaattatt
     1081 gaaagccaat ccaggtttca aaatctacct tacgcaaggc tttatttgcc gtaattgtta
     1141 tggagaaatt gataatttac aacgtggtgg aagtgattat accgcttcat taattggtgc
     1201 agcggttgaa gcagaagaaa ttcaaatatg gacagatatt gatggtttgc acaacaacga
     1261 cccgcgttat gtaaaaaata caaaagccat tccaatgctt aattttgaag aagcagcaga
     1321 attagcttat tttggagcca aaattttgca tccaacatgt attctgcctg caaaacttgc
     1381 caatattcct gtgcgtttac tcaatacatt acagcctgaa gctccaggaa ctcttatttc
     1441 aacacaaatc aataaaggta aaattgaagc agttgctgca aaagatggta tcacagccat
     1501 taaaatcaaa tcaggaagga tgctcttagc atatggtttt ttacgaaaag tgtttgaaat
     1561 tttcgaatca tataaaacac caattgacat gattactact tcagaagtcg gtgtttctgt
     1621 aacaatagac aatacaaaat atctggaaga aattgtaaac gacttaaaaa agttaggaac
     1681 tgtttctgta gataaggata tggttattgt ttgtgtggtg ggtgacttat tttatgaaaa
     1741 cgtaggtttt caagctcaaa tagtggaagc tttaaaggat atacctatcc gaatgatttc
     1801 ttacggcggc agtaattaca atatttcttt tctcattaaa aaagaagata agggtaaagc
     1861 gctgaacgct ttaagtgatg ctcttttcaa ttaaaaaagt aatgttgata taaaaagtat
     1921 tggctctttc tttttaaaaa ttatgatcaa agcacaatat cccatagaaa aattttcaaa
     1981 attacagact ccgttttatt attacgatct ggatattcta cgcaaaactt tacaggaagt
     2041 gaacaagcag attcaaaaaa gtgattatat tgttcattac gccataaaag ccaatgctaa
     2101 tccggcttta cttcacgaaa taaaagatac aggatttgga gcagactgtg taagcggagg
     2161 agaaataaat gctgcaataa aagcaggtat tcctgcttca aatattgtgt ttgctggggt
     2221 tggaaaaaca gattgggaaa taaatcttgc attggacaaa gacatttttt gtttcaatgt
     2281 agaatctatt cccgagcttg aagtaattaa tcagttagcc aaagcaaaag gaaaaattac
     2341 aaacattgct ttgcgtctca atccaaacat caatcctcat acgcatcact atattaccac
     2401 aggattggaa gaaaataaat ttggcattaa tctctctgat ctggacgaag taatgaattt
     2461 gcttcctaaa ctgaaaaatt taaaactcat tggcgttcac gcacacattg gctctcaaat
     2521 aaccgatatg agctctttta aaagcttatg cataagaatc aatgaaattc agcaatattt
     2581 ttttgcaaga agaatcattc tcgaacacgt aaatgttggc ggtggattag gtgttaatta
     2641 cgaacaccct aaccattttc ccattcccga ctttgaaagt tattttaatg tatttcggca
     2701 acatttgctg ttgcagccgc aacaaattct ccattttgaa ttgggacgat ccattgttgc
     2761 ccattgtggc agtttgattt caagagtaac ttttgtaaaa aaaggagcta caaaaaagtt
     2821 tgctattctg gatgctggtt ttaccgattt aattcgtcca gcgttgtatc aagcttatca
     2881 tcgcattgaa aatcttactt ccgatttacc ggaagaagaa tacgatgtag tgggaccgat
     2941 ttgcgaatcg tccgatgttt ttgcaaaaaa ttatcccatg aatcaagcta gaagaggcga
     3001 tttgattgct ttgcgttctg ctggtgctta tggcgaaagc atggcttcac aatataattt
     3061 gcgaccttta ccaaaaacat atacttccga tcaactttta ttggaagata attaagatat
     3121 taataaacaa tgattgtcca ataaatctat tttattctgt aatgtttttt caatggaaat
     3181 atttaacatt tatttttctc gggctgaatt atatttttta ggtggattac tattaacttt
     3241 tatttatcag atttattttt atttccatta catcaatggc gttattcatt acagaaaaaa
     3301 aattcaacat cagaaagtat cttttctcga aaaacagccg cccgtttcgg taattatttg
     3361 tgcaaaaaat gaagcggaaa atttaagcaa atttttgcct gccattttag aacaggatta
     3421 tcctgatttt gaagttattg tcattaatga tggttctgtt gatgataccg acattttgtt
     3481 gggagcattc agtaagaaat atcccaactt acgaaccact ttcgttccac aggaagcaca
     3541 aaacctcagc accaaaaaat taggaattac gttaggtgta aaagctgcaa aaaatgattt
     3601 gcttctcttc accgacgccg actgcgtacc agcaagccac cagtggataa aattaatggt
     3661 gcgaaacttt ttacctgata ttgaatttgt tttaggatac agtggttatt cttctcaaaa
     3721 aggttttctc aatcaattaa ttacttacga tacgttattt attggtttgc aatatatggg
     3781 catggcactg gcagggaaac cttatatggg agtaggtaga aatctggcat acaggaagca
     3841 aacttttttt gatcagaatg gctttgcttc cagtttaaat cttcttgctg gcgatgatga
     3901 tctgatggta aaccgggctg ccaataaaaa gaatactcgt gtagaaattt cccctgaaag
     3961 tattacctgg tctcgaccaa aaactacttt caaaagctgg tatcagcaaa aaaagcggca
     4021 tctctccgta tcatcggctt atactaaaaa aagtaaatgg agattaatgt tagaacctct
     4081 gacacgtggt ttgttttata ttttattgat tgttacttgt attgttggaa ataacattac
     4141 aaaaattttt gctgtttttc tatttttggt tcgatatgca attcaaatta ctattataaa
     4201 tcgttcagcc cgacatttta aagaacgtca atattttctt acgctgcttg tttttgatct
     4261 tttgcttccc ttaattcctt tgtctatttt agcaacaaac agaaaattaa gcagatataa
     4321 taaaaaatgg tagtaaaaaa atcaaaaaat ttcttttgaa ggatttactt taaagaaagt
     4381 aaaatttact tttccgtagc ttcgttgttc tacaaaatga ggatggtgtt ggaaattgta
     4441 ttttgccgaa tgttcaagca cgaaaatccc attgggcttt agcaaatgat aagaaaaaat
     4501 aagatcggga atttcagcaa tatttttcag taaataagga ggatcagcaa aaatcatatc
     4561 atattgagcg tgtgtagagg caatatattt gaaaacatct gttctgaaaa gaaaaagatt
     4621 atcaattttc aaatcactga caacttttct aataaaagca cattgattct tatctgtttc
     4681 aatgctcact accttattgc agccacgcga aacaaattca aatccaatac ctccagtccc
     4741 tgcaaacaaa tccagcacat caattccttc taagtcaatt ctatttatca ataaatcgaa
     4801 caagccttcc tttgcaaaat cagtagtagg tcgcgctgaa atatttatcg gggcataaat
     4861 tttcctacct ttatattttc cactgataat tctcatattt attccctctt tttctctata
     4921 atttcacaat gcttcatata tttttgtgct aaattatata attttgaatg acgcttttca
     4981 taattgaaca atcttatttt acaaccatca atatcaaaag aaagttcttc tatcactcgc
     5041 aatagataat aaacaaagtc ttcaggagtt tgaaatacaa aactatttat taacatcaac
     5101 tgattgtttt caatagcaac tacgtccatt aaatgctctc gaagatacac attcaaattt
     5161 tcctccttat ttgtataagc ttgtaaatta attaaaaatt gggtaatatg atgttcaata
     5221 ttcaactttg gaaaaaattg ttcgaaaact tcatatataa tgctcggcac agaaaaaatg
     5281 tttacagttt caaaagaagc aatcggatta gataacacta tttcattttt ttctttttta
     5341 tgatgaaagt aaaacaaact ctcagcctct acctttttaa aaatcatttg aggaacaaca
     5401 gaataacgat ttgattcata agttaattta acgttgtgat aaggaagttg catttccggt
     5461 tctttgagca aaaggggaat caactcctct ttgttcatga caaaaagagg agcttttata
     5521 tttttcaacg aaatcaaacg tttttgatca tcatagacaa agaaagaaaa tccattcggc
     5581 ttttgccgaa tggataaatt atatagatta ctctctttaa aaaatttctc ttgagaagat
     5641 atgttttcag aaagcatgtt acatgtttgt aactggaaat tattcccagt tcccagcatt
     5701 attgttcggc tcaacaatag aacctacctt taaaccagga tatttattta atttcttagc
     5761 acgatcaatt aagttgagca attcttgtcg gttcaaatca tgtaaataag tttcataagg
     5821 tgcacatgcc tcaaataagg ggattctaat tcccgattta tttacataat cattatttac
     5881 tcttaattca aatttttccc cttgagaata aggtatataa catatttgat cgatagtttg
     5941 acgggtataa ttaggaaata cctcttctaa aagatccact tttattgtat cccgacgaaa
     6001 attttgcaaa ccatattttt caatttcctg tttatttcct cgacgaacaa tttgagctgc
     6061 tttttcctct gttaagcctg cttcgagctg ttcatcagta aggaaacctt cttttaatac
     6121 aattttcttc tttgctgtct ttagaaaatt tatcaaagta tcaaaacttg ccgtatatct
     6181 tccgttttgt tctttaaatt caacttcagc tttacgaatt tctattaacc gttgaattac
     6241 atctttctcc ctttgagccc ttatcctctc aaaattaata ggagtaagaa tactcatcac
     6301 acataaataa gctaaaaaaa tagcagcaag tgataataaa actttaatgg ttgttttcat
     6361 gtttaataaa tgtaatttaa atttatgaaa tatggtttta aaatattttt tttaccttaa
     6421 ttataataat tgcaaattta aaaaagaaaa ttaaatatac cattttcttc tatcaatttt
     6481 ttttattttt tcttttaatg ctatttttta ccattttttc aattattttt ttgactttaa
     6541 ctcaaaagat gtgttataca tctgtaataa attagcaaaa gccgaaattt tttaaataag
     6601 ctatattatc ttttaatgac atataaaaat tttttgaaac aaatttgata tgacttctta
     6661 ttcaacatta cccgtttcag ttttatatgg aaaagtctta caagccagtg aaaatctttt
     6721 tagcttcaca gagcgaaaag aaataaaaaa tatactgaaa cagtattgca aaagccatcc
     6781 tttattattt gtaaaaaata ataattcaat agaagataaa ctgtctgtta tccaaattct
     6841 tttaacagaa ataggcttag gaaaagaaat ggtaatcagc attttatttt ttgatgtttt
     6901 aaaggaagga cttatcagta tttctgaaat tgaacagaaa tttcatataa aaatttctcc
     6961 cattatagag gggctggaaa aagtcaatga actatacagc aaaaaaacaa ctcttgagac
     7021 agaaaatttt agtcaatttc ttttatcggt tgcaaaagat gttcgtgtcg tattgattct
     7081 gatcgttcaa aaattatata ctttacgcac actcgaaaaa ttttctgttg aaaagcaaca
     7141 agaaatagct caggaatgtg cagtgttgta tgctccttta gctcatcgat tggggttata
     7201 caaaattaaa agtgaattag aagatctggc attaaaatat actaatcgcg aagtttacaa
     7261 agaaattgcc aataaattaa atgagactaa acgctcacgc gatcaataca ttgcagaatt
     7321 tattgctcct cttgaagaaa aacttcgtca agaaattgaa tatccatttg aaattaaagg
     7381 aagaacaaaa tctatttttt ccatttacaa taaaataaaa aaacaaaata ctacttttga
     7441 aaatatctat gatttgtttg ccattcgtat tattcttgat gttccgcttg aaaaagaaaa
     7501 aatgatttgc tggcaagttt attctattgt aacagatatg tatcaaccta atcctaaacg
     7561 attgcgtgat tggatttcta ttcctaaatc taatggttat gagagtcttc atattacggt
     7621 attaggacca caaaataaat ggatcgaaat acaaattcgc accacacgga tgaatgaaat
     7681 tgcggagaaa ggattagcag cccactggaa atataaaggt atccagagtg aatctgaaat
     7741 ggataaatgg ttaaaaaata ttcgtgatat acttgaaaat ccacaattaa atgctattga
     7801 cttgctggat gagtttaaac tgaatttgta cgataaggaa gtttttgttt ttactcctac
     7861 tggagaatta ttacggtttc ccaaaggtgc aacagtactt gattttgcat tttatattca
     7921 taccaatttg ggtttgaaat gcacaggagc tctaattacc aacaatactt atcaggaaaa
     7981 aaatgtacca cttaaatatg ttcttaaaaa tggagatcaa gtaaaaatac ttacttcggc
     8041 aacgcaaaag ccgagtgccg aatggttaaa tatcgttgct acctcaaaag ccaaagcaaa
     8101 aattcgtcaa gcattaaaag aaattcaata caaagatgca gttatcggac gagaaacact
     8161 tttgcgacgt ttcaaaaatt ggaaaattga gctggatgaa ggtaaaattt ctcgtctggc
     8221 aaagaaaaaa ggatataaga cagtaagtga tttttatcag gaaattgcac aggaaaaact
     8281 tgatcttttg gctcttcggg atacctattt ggagatggaa aagcatgaag ctcagcaaaa
     8341 tgtaacggaa acacgaagtg ctgagaattt cataaaagag ccgcccgtta ttgaaacctc
     8401 tatcaaagaa aaagaagata ttttaattat tgatcaaaat ttaaaaggaa ttgattatac
     8461 gctggcaaaa tgttgtcaac caatttatgg agatgaaatt tttggatttg tgtctgttgg
     8521 gggtggcatc aagattcaca gagtagattg tcccaatgca cctgaattgt attcaaaatt
     8581 tggttacaga attgtaaaag ctcaatggtc aggacaatca ggtggcactc aatatcccat
     8641 tactttacac gttattggga aagatgatat tggcatcgta accaacatta cttctttaat
     8701 tgacaaggaa aaaaacattg tgttacgctc tatttccatt gattcaaaag caggactttt
     8761 tattggaaac ctgactatta tggttaatga tgctcatgat ttagatggca ttatgaaaaa
     8821 aatcagcatg ataaaaggaa ttgtaaatgt ttcacgaata aaaaccatta ataagtataa
     8881 ctcataaaaa atttaagaaa tgttttcagg aataattgaa gaagctgctc cagtagtggc
     8941 attaaaaaaa gaagaaggaa atctgcacat taccatgagc tgttcttttg taaatgaatt
     9001 gactattgac caaagtattg ctcacaatgg cgtttgcttg acagtggttt caaaaaccga
     9061 tgatagttat acagtaactg ccattcagga aacacttgat cgaacaaatc tgggagaact
     9121 ggtttgtgga aacgaagtaa atctggaacg cagtttactt ttaacggaac gactggacgg
     9181 acatattgtg cagggacacg tggatactac tgctgtttgt actgctattg atgaagtaga
     9241 aggaagctgg tattttacgt tcgaatacca atttgatccc gaaatggcga aacgcggtta
     9301 tcttactgta gataaaggtt cagttgctgt gaatggcgta agtcttactg tttgcaatcc
     9361 aaccgataac tcttttcagg tagccatcat tccttatact tatgaacata ctaacttcca
     9421 tcaaataaaa gtaggtacta cggttaacat tgaatttgac attattggca aatatatcag
     9481 ccgattgatg aagtacaatg tttgagattc tcacagttgt tatcaaacca aaagcttgca
     9541 atgattgtta taggtagtaa aatgtagaag attgaattga ctacctatgg ataaattaag
     9601 aaaacaaatc aaagaatctt tctttaatcc aatttatcat ttcttcccgc taatcgtttt
     9661 cttgatagta gataatgtgg cgggaagaaa ttgggcatgg tcaattgcta tccctttatt
     9721 ccttatactt acaatttatc tttttattgc ttataaccag ctttttgtct ggtatttttt
     9781 ttctgctttt atttttcttg ccgtaaatct gagtgcaatt tttactttct tatcagaaaa
     9841 atggctattt ctttcaccca ttatagatga tttagtttgc tggctatttt taggtttgac
     9901 attgttattt cgctctttca ttgaaaaaaa aatcaatgca agaactccga aatttttacc
     9961 catgaaaaac aattttgaag aatattttaa tttcattcaa tctttatttc taatcatttc
    10021 cggttatctt ctcttttatt tcattattta tcaattccca acaataaaag aaaacatcta
    10081 tttagaatta cttcaagtaa tttatattgg agcaattgtt tttctgctgg tttttgaatt
    10141 ttgccgtgtc caaattgttc gccataaact attaaaagaa gaatggtggc ctattgtaaa
    10201 cgaacaggga aaaattgttg gaaatgttca acctctggta agtttaaaaa ttgaaaaaaa
    10261 atacatgcat cctattattc gaattttatt tattaaagaa aatatgattt ttctgcaaaa
    10321 aaactgtgaa aatgatttaa tttttccctg tttatgggat gctgccattt ctaatcatat
    10381 ccgcacagga gaaacaattg aagattgtat tgaaagaaca gcagaagaaa aatatggatt
    10441 aaaagatttg aagccttttt tcctttcaaa ctatattcat cgtactgaaa atgaacaaca
    10501 ttatgctttt ctttttattg cttgcaatgt aaataacata gagcctaatt atcaaaaaat
    10561 aaatcaagct aaatggtgga cgctgcaaca aatagaagaa aacctatcga caggaatatt
    10621 tacccaaaat tttatcatcg aatacaattt attacaacgt agcggagtgt ttgaaaataa
    10681 aatatgtaac tgcgaatgta aactaaaaga attgttttct taatatcaat tgccatgaat
    10741 ttcttcccat catcttcagc gaagggaata ttatcttaac tttgcattgt ttaattttat
    10801 tcatataaaa tgaaagcaat atgacaacat tagtagatcg ttttttaaaa tatgtagaat
    10861 ttaccactac ttctgatgaa aacaccaatt taacccccag cacacctggt caactggcac
    10921 ttgctcatta tctggtaaaa gaattaaaat caataggact aaaagatgct gaagtggaca
    10981 aaaatggtta tgtaatggct actttacctg ccaatattga taaagaaatt cctaccattg
    11041 gttttattgc tcatctggat accagccccg atatgagtgg caaaaatgta aaaccaaaaa
    11101 ttatcaaaaa ttatgatggg aaagatattc tgctgaatga agaaaagctg attgtttttg
    11161 aaacagaaaa atttcctgaa attcttcaat atattggtca agatattatt gttactgatg
    11221 gaaccacgct gttaggagct gatgacaaag ctggaattgc agaaattatt tcggctatgg
    11281 aatatcttgt tgaccatcct gaaattaaac atggaaaaat ccgtattgct ttcactcctg
    11341 atgaagaaat cgggaaagga gtagattatt tcaatgtaaa aaaatttaat gcacaatggg
    11401 cttataccat cgatggagga gaaattggag aattgcaata tgaaaatttc aatgcagctt
    11461 ctgccaaatt gacttttaat ggtttgaatg tccatccggg ttatgcccgt cataaaatga
    11521 aaaattctat tcgcattgct caacaataca tgagcatgct tccccgacac gaaacgcccg
    11581 aacatacaga aggttatgaa ggtttttacc atattaccaa tatagaaggc accgttgaaa
    11641 aaactacgtt aaactgcctt atccgtgacc atgatcgcga taaattcgaa agaagaaaaa
    11701 aggaattaca acatcttgtc aataaaataa atgctgaatt tggcgaaaat acagcaatta
    11761 ttgaattaaa agatcaatat tacaacatgc gtgaaaaaat agagcccgta atgtatatca
    11821 ttgatatagc ttcacaagcc atgaaagaag ttggtgtaac cccgaaaatt tctccgattc
    11881 gtggtggcac cgacggagca cgactttctt atatggaact cccctgccct aacatttttg
    11941 caggaggaca taattttcat ggacgttttg aatacattcc cattccttct atggaaaaag
    12001 ccatgatggt tattgtaaaa attgcagaac tggttgacaa tcaagaaaat atttctcaat
    12061 aattttatca aaaatcattt ttatttattt ttcagaaaaa attcaaaatc aaaaaattca
    12121 tcaatgaaaa ccactcc
//
LOCUS       DAOSAS010000042        11981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3580_length_11981_cov_14.029935, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000042 DAOSAS010000000
VERSION     DAOSAS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11981
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3580_length_11981_cov_14.029935"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PLR72_03125"
     CDS             complement(<1..503)
                     /locus_tag="PLR72_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOK36234.1"
                     /translation="MKKFVYSAIFLLCGIFAYGQIDFGFKLGYNSSVGLNDLNSITKG
                     KYTLDSAKIDAKNNFMVGAFSRINFGKIYLQPELLYVGGKKNYSETVIVSEGNTASYD
                     KKVTMSMIDIPVLLGYKLLDLKLANVRVFIGPKFRFNAGSKFKVENFNSTGEISKDDI
                     TKEIKKS"
     gene            792..1244
                     /gene="coaD"
                     /locus_tag="PLR72_03130"
     CDS             792..1244
                     /gene="coaD"
                     /locus_tag="PLR72_03130"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOK36235.1"
                     /translation="MKRAIFPGTFDPFTIGHYSIVQRGLKLFDEIVIAIGTNQAKKTF
                     FSLEKRREIINQAFVNEPRVKIMIYDSLTVDFARSIQANFILRGLRSVTDFEYEHSIA
                     DANRKIAGIETVILFTEYEYSYISSTVVRDLISYGKDISAFLPPNVQL"
     gene            1267..2919
                     /locus_tag="PLR72_03135"
     CDS             1267..2919
                     /locus_tag="PLR72_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOK36236.1"
                     /translation="MKKTSLLSFLLLLIPFIIFAQNQTSSRTFRISKNLNIYNSIFRE
                     LDMFYVDTLNYDKIVRTSIDKMLDNLDPYTVYIPEEETEDLTFMTTGEYAGIGALIMK
                     KGDEVVVSEVYEGMPAQRNDIRAGDVILEVDGQKTTGMSVDKVSALLRGFPNTQIKIK
                     IKRYGEKKIIEKKFIREKIQIKPIVYAGQVAEKIGYIQLGEFTDGVAFELKSSLNELV
                     KNYHITSLVLDIRSNGGGLVDEAVKILGYFLPKGTEVVTTRGKNKELDRIYKTPTEPI
                     YPDMKLAILVNRSSASASEILAGAIQDLDRGVIIGERTFGKGLVQNIRAINFGGHLKV
                     TTAKYYIPSGRCIQAIDYSHRNEDGSVGYVPDSLTSEFSTRNGRKVRDGGGIVPDIPT
                     DSSRKLNIAHYIYAQNLYFDFATQYAYQHPTIAPPESFELSEKDFNDFINFLKERNFT
                     YTSQTEKYYNQLYQIAEIEGLDSIAQPEFEALKKKLTPDIDKNIQANKNDIVDFLSLE
                     IIKRYYFQKGQIQYSLRNDNDLQSALDVLQSEDKYKKILSII"
     gene            3050..3955
                     /locus_tag="PLR72_03140"
     CDS             3050..3955
                     /locus_tag="PLR72_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOK36237.1"
                     /translation="MKPTLLILAAGMGSRYGGLKQLDGIGPHGETIMDYSIYDAIKAG
                     FGKVVFVIRKSFEKEFNELIIKKFKNLIDIEVVFQDLDKIPEGYTYSPRREKPWGTNH
                     AVLMAKDVIHEPFGVINADDFYGRESFEILSNFLQSVTDKKNEYCMIGYRVGNTLSEN
                     GPVSRGICIVNEKNELINVVERTHIQEKGGTIIYLDENGREVTIPPETTVSMNMWGFT
                     PDYFDYSLEYFKKFLEENSQNIKAEFYIPTVVNQLVNEKKVICKVIETPSKWFGITYA
                     EDKPQTILKINELIHKGIYPQKLFD"
     gene            3966..5027
                     /gene="galE"
                     /locus_tag="PLR72_03145"
     CDS             3966..5027
                     /gene="galE"
                     /locus_tag="PLR72_03145"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOK36238.1"
                     /translation="MMTKVLVTGGTGYIGSHTVVELQQEGFEVIIVDNLCNSNIEVLN
                     GIEKITGIRPMFENIDCTDYINMDRMFEKYQNIKAIVHFAALKAVGESVEKPLMYYRN
                     NLVSLINLLQLMPVHKIKNIVFSSSCTVYGQPDVLPVREDTPFKPALSPYGNTKQICE
                     EIIRDTIHANPNLYRSIVLRYFNPIGAHPSAEIGELPIGVPNNLLPFITQTAIGIRKQ
                     LLVYGNDYNTPDGTCIRDYIDVVDLAKAHVIALKRMLKQQQKTNLEVFNLGTGRGLSV
                     LEIIKTFEKVNGVKVPYKIVGRRPGDIEKVWADPSLANKELGWKAERTLEETLRSAWE
                     WEKKLASRAKEKDHTKTES"
     gene            5043..6017
                     /locus_tag="PLR72_03150"
     CDS             5043..6017
                     /locus_tag="PLR72_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOK36239.1"
                     /translation="MLYPLKFHPILKEKIWGGSRLKKLFNKPTESDKIGESWELSGYP
                     GDESVVANGFLAGNQLNELIEVYMDELVGGKVFDRYGLTFPLLFKFIDANDDLSIQVH
                     PNDETALKKHDSLGKTEMWYVVDADENGELIVGFKNDCSAAEFLTALNEKNLEDLLQR
                     VSVKKGDVIFIHPGLVHAIKKGVVVAEIQENSDLTYRIYDYQRKDENGKERDLHLEAA
                     LEVIDFSACKNPLVDYVEKMNEVTTLVSCQYFTTNLLAFNQNLLRNYVQIDSFVVYMC
                     LEGNFIIEYENSRTPVEKGETVLIPASMDEVNLIPAEGTVKLLEVYID"
     gene            complement(6073..6597)
                     /locus_tag="PLR72_03155"
     CDS             complement(6073..6597)
                     /locus_tag="PLR72_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK36240.1"
                     /translation="MRAVEPEDLEKLYKWENNPQLWLVGNARNPYSHFTLKQYILNSD
                     KDIYENKQLRLMIVLKENNETIGTIDLFDFDIHNSRISLGIYVENKFQRKGYATDSLR
                     LVQEYVFDYLKINQIYAFVAEKNTPSIHIFEQEKFEKNGILKNWIKTLDDFQNIFVFQ
                     KFRENYLLQKNKIT"
     gene            complement(6626..7654)
                     /gene="recA"
                     /locus_tag="PLR72_03160"
     CDS             complement(6626..7654)
                     /gene="recA"
                     /locus_tag="PLR72_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOK36241.1"
                     /translation="MAEKTADEIKTTSPNAEKLKALQLAMDKIEKDYGKGTIMRMGDN
                     KVVDIDVIPTGSLGLDIALGVGGYPRGRIIEIYGPESSGKTTLAIHAIAEAQKAGGIA
                     AFIDAEHAFDRAYAENLGVNTDELLISQPDNGEQALEIADQLIRSSAVDIIVIDSVAA
                     LTPKAELEGDMGDAKLGLQARLMSQALRKLTANISKTKTTCIFINQLREKIGVMFGNP
                     ETTTGGNALKFYSSVRLDIRRVTPIKDGEEVIGYQTRVKVVKNKVAPPFRKAEFDIMF
                     GEGISKAGEILDLGVDYGIIKKSGSWFSYNDTKLAQGRDAAKTVIKDNPELAAELEAK
                     IKEAIKQK"
     gene            complement(7733..>8923)
                     /locus_tag="PLR72_03165"
     CDS             complement(7733..>8923)
                     /locus_tag="PLR72_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOK36242.1"
                     /translation="LEEALRNRNIPYRIYGGLSFYERKEIKDVIAYCRLICNQQDEEA
                     LKRIINYPTRGIGETTLNKIIECAQQNQISLWEVISNLAEYKLPINSRTVTKIEQFKE
                     LINVFVGQVNTQNAYDLVNQLINVTGIFQDIYSDNSPENLSKQENIQELLKALHEFCE
                     RKEKEEDKGFLPDFLSEISLLTDQDTDKSEGDRVTLMTVHSAKGLEFRAVFIVGMEEQ
                     LFPSIFSIFNERELEEERRLFYVAITRAKEHCFISYAKSRFRNGKTYFSEPSRFIKDI
                     DKQYLDLPSEETISQQTSTSWDDTLVTERKHFYQQSNGQGTVENSFSQKRLVKLNDLK
                     EEKVLIEKPSLPVGSLVKHSQFGIGKVIETCIVNGNERAEIDFGEKGKKSLLLKFAKL
                     EVLK"
     assembly_gap    8924..9023
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9024..9896)
                     /locus_tag="PLR72_03170"
     CDS             complement(<9024..9896)
                     /locus_tag="PLR72_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOK36243.1"
                     /translation="MIEREKDTNFDFLNELNESQRLAVEYTDGPSLVIAGAGSGKTRV
                     LTYKVAYLLKKGFPPSSILALTFTNKAAREMKERIAEIVGNKIARYLWMGTFHSIFSR
                     ILRAEAQYTGFTQNFSIYDQSDSKSLLRSIIKEMELDSKIYTPGLVQSRISMAKNNLI
                     TPKAYAADYSLIEKDRKNHVGKLCDVYQTYCIRCKQANAMDFDDLLLQTNLLFRDHPE
                     VLQKYQQLFRYILVDEYQDTNFAQYLIIKKLAERHKNICVVGDDAQSIYSFRGANIDN
                     IFQFKNSYQDAKIFK"
     gene            10154..10702
                     /locus_tag="PLR72_03175"
     CDS             10154..10702
                     /locus_tag="PLR72_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOK36244.1"
                     /translation="MKKLVRLIFKYFGWQMIYPLPEPQKSVICLAPHTSNWDFVIGEL
                     SYWSIGQKASFLIKKSWFIFPLNFFFKSLGGIPIDRSKHNSLTQQMIDKFQKLDSFHL
                     VITPEGTRGYVTAWKKGFYYIALGANVPIQLSYIDYKKKELGITKIFYPTGNIDADFQ
                     EIQNFYESVQPKYPEKYNRKIF"
     gene            10713..11486
                     /locus_tag="PLR72_03180"
     CDS             10713..11486
                     /locus_tag="PLR72_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOK36245.1"
                     /translation="MKVTLIQDEIFWADKTTNLRKVGQQLEFLSGKTDLVILPEMFST
                     GFCTDQLNLAETMEGETIQMLRSYALKYQLAITGSFIATENGKFYNRAFFIFPTGEMV
                     TADKRHLFQAGGENKFFTKGNKRLQVSYYGFNICVLVCYDVRFPVWSRNVNNEYDLLI
                     YVANFPLKRINDWDVLLQARAIENQTYVAGVNRIGTDGLGIEYNGHSALLDFKGRPLL
                     AFPENEFSIQTTELDKAPLISYRERFPVWKDADIFEIKK"
BASE COUNT         3955 a         1865 c         1971 g         4090 t
ORIGIN      
        1 tgagattttt ttatttcttt tgttatgtca tccttactaa tttctccagt agaattaaaa
       61 ttttctactt tgaattttga acctgcatta aaacgaaatt ttggtcctat aaataccctt
      121 acattggcta attttaaatc caacagttta taacccaaaa gtacaggaat atcaatcata
      181 ctcatagtca cctttttatc gtaacttgcg gtattacctt ctgagacaat aacagtttct
      241 gaataatttt tcttgccgcc tacatataat aattcaggtt gtaaatatat tttcccaaaa
      301 tttatgcgag aaaaagcacc taccataaag ttattttttg catcaatttt tgcagaatcc
      361 aatgtatatt ttcctttagt aatggaattc aaatcattca atccaacgga agaattatat
      421 cctaacttaa agccaaaatc tatttgacca taggcaaaaa taccacatag tagaaaaatt
      481 gccgaataaa cgaacttttt cataatattt tacttttaaa attaaactaa tttcaaagta
      541 aagaaaatta ttttaaaaaa tatccttctt tgtcaaaata ttttcatttt tttaattttt
      601 caactgaatt tctatcaata atgcaaaata aaaaatactt tttatatgaa aaaaatactt
      661 cataaattaa aaaatataaa cttatcttag tgatttggta ttactgagag caaatttttt
      721 atctttgtaa ttcaaatttt ttattaagta ctttaatttc aaatattaat ttcaacgtta
      781 atactgtatt tatgaaacgt gctattttcc cagggacttt tgaccctttt acaattggtc
      841 attacagtat tgtgcaacgt ggtttgaagt tgtttgatga aatagtaatt gccattggaa
      901 ccaatcaggc aaaaaaaact tttttttctc tcgaaaaaag aagagaaatt atcaatcaag
      961 cattcgtaaa tgagccaaga gtaaaaatca tgatttacga cagtttaacg gtggattttg
     1021 ctcgctccat ccaagctaac tttatccttc gagggctgag atcggtgacc gattttgaat
     1081 acgaacacag tatagctgat gctaaccgaa aaattgctgg tattgaaaca gttattttat
     1141 tcaccgaata tgaatattca tacatttcct ctacggtagt tcgtgacttg atttcttatg
     1201 gaaaagatat ttcagcattt ttacctccca atgttcaact ttaataaata tttttattct
     1261 gactttatga aaaaaaccag tcttttatca tttttgctgt tactaatacc tttcatcatt
     1321 tttgctcaaa atcaaaccag tagccgaact tttcgtatta gtaaaaacct caatatttat
     1381 aattctattt tcagagaatt ggatatgttt tatgttgaca cacttaatta tgataaaata
     1441 gtaagaactt ctattgataa aatgttagat aatctagatc cttatacggt ttatattcct
     1501 gaggaagaaa cagaagattt aacttttatg actactggtg aatatgcagg tattggtgct
     1561 ttaattatga aaaaaggtga cgaagtagtg gtttcggaag tatatgaagg tatgccagct
     1621 caacgaaacg atattagagc gggagatgtt attttggaag ttgatgggca aaaaacaaca
     1681 ggaatgagtg tagataaagt tagtgcttta ttgcgtggct tcccaaatac tcaaataaaa
     1741 atcaaaataa agcgttacgg tgaaaaaaag atcatcgaaa aaaaatttat tcgtgaaaaa
     1801 attcaaatta aacctattgt ttatgcaggg caagtggcag aaaagattgg ttatattcag
     1861 ttgggagaat ttacagatgg cgttgctttt gaattaaaat catctttaaa tgagcttgtg
     1921 aaaaattatc atatcacttc tttagtcctt gatatccgta gcaatggagg aggtctggtt
     1981 gatgaagctg ttaaaatttt aggatatttt cttcccaaag gaacagaagt agtaacaaca
     2041 agagggaaaa acaaggaatt ggatcgaatt tacaaaacac ctactgagcc tatttatcct
     2101 gacatgaaac tggctatttt ggttaatcgt tcttcagcat cagcttctga aattttggct
     2161 ggtgcaattc aagatctgga cagaggagtt attattggcg aacgtacttt cggaaaagga
     2221 ctggttcaaa atatacgagc tatcaatttt ggaggacatt taaaagtaac tactgcaaaa
     2281 tattatattc ccagtggtag atgtattcaa gccattgatt acagtcatcg aaacgaagat
     2341 ggtagtgtgg gttatgttcc cgatagtttg acttcagaat tttctactcg aaatggtcga
     2401 aaagtacgcg atggcggagg cattgtacct gatatcccga cggatagtag tcgaaaactc
     2461 aatattgccc attacattta tgctcaaaat ttatattttg attttgctac tcaatatgct
     2521 tatcaacatc ctactattgc accacctgag agttttgaac tttctgaaaa agatttcaat
     2581 gactttataa attttttaaa agaaagaaat tttacttata catcacaaac tgaaaaatat
     2641 tataatcagc tttaccagat agcagaaatt gaaggactgg acagcattgc tcaaccagaa
     2701 tttgaagcat tgaagaaaaa attgacacct gatatagata aaaatattca agctaataaa
     2761 aatgatattg ttgatttttt aagcttggaa atcattaaaa gatattactt tcaaaaaggg
     2821 caaattcaat attcgctgag aaatgataat gatttgcaaa gtgctcttga tgttcttcaa
     2881 tcagaagata aatataaaaa aattctttca attatataga atatgcagta caccaaactt
     2941 ttcaattgaa attgataaag ttatcacaaa tgttatgaca ttttctaatt tcaattgaaa
     3001 aaaatatcac atatacatgg actcaatttt taaattttga caattaatta tgaaacctac
     3061 attattgata ttagcagcag gaatgggaag ccgttatggt ggactaaaac aattggatgg
     3121 catcggacct catggtgaaa caattatgga ttactctatt tatgatgcca tcaaagccgg
     3181 ttttgggaaa gtggtatttg tcatccgcaa aagttttgaa aaagaattca atgagttgat
     3241 cattaaaaaa tttaaaaatt tgatagacat cgaggttgtt tttcaagatt tagacaaaat
     3301 acccgaaggt tatacttatt cacctcgtcg cgaaaaacca tggggaacaa atcatgctgt
     3361 gttgatggct aaagatgtga ttcacgaacc ttttggagta attaatgcag atgattttta
     3421 tggcagagaa tcatttgaga ttttatcaaa ttttttacaa agtgtaaccg ataaaaaaaa
     3481 tgaatattgt atgataggtt atcgagtagg aaatactctc tctgaaaatg gacctgtgag
     3541 ccgtggtatt tgtattgtta atgaaaaaaa cgaattaatt aatgtggtag aacgaaccca
     3601 tattcaagaa aaaggaggaa ctatcatata tttagatgaa aatggtagag aagttacaat
     3661 tccacctgag acaactgtat cgatgaatat gtggggtttt actcctgatt attttgatta
     3721 ttctctggaa tattttaaaa agtttttaga agaaaacagt caaaacataa aagctgaatt
     3781 ttatattcct acggtggtca atcaacttgt aaatgaaaaa aaggtgattt gtaaagttat
     3841 tgaaactcct tcaaaatggt ttggaatcac ttatgcagaa gacaaacctc aaactatact
     3901 gaaaatcaac gaattaatac ataaaggcat ttacccacaa aagttgtttg attaaaaatt
     3961 tttttattat gactaaagtt ttagtaactg gaggcacagg ttatattggt tctcatactg
     4021 tagtagaatt acagcaggaa ggttttgaag tgattattgt tgataatctt tgtaactcca
     4081 acatagaagt attgaatggt attgaaaaaa ttacgggcat tcgacccatg tttgaaaata
     4141 ttgactgcac ggattatata aatatggatc gaatgtttga aaaatatcaa aatattaaag
     4201 caattgttca ttttgcagct ttaaaagcgg taggagaatc ggtggaaaaa cctttaatgt
     4261 attatcgtaa caatttggtt tctttaatca atcttctcca attgatgcct gtccacaaga
     4321 taaagaatat cgttttttca tcttcatgta cggtttatgg acagcctgat gttttgcctg
     4381 ttagagaaga tacccctttt aaaccagcgt tatcacctta tggtaataca aaacaaattt
     4441 gtgaagaaat cataagagac actattcatg ccaatcctaa cctttaccga agtattgtat
     4501 tgagatattt taatccaatc ggtgctcatc cttcggctga aattggtgaa ttaccaatcg
     4561 gtgttcctaa taatttatta ccatttatta cccaaacagc tattggtatt cgtaaacagc
     4621 ttctggttta tggaaatgat tataacacgc ctgatggcac ttgtattcgt gattacattg
     4681 atgtagttga tttagccaaa gctcatgtta ttgctttaaa acgcatgcta aaacaacaac
     4741 aaaaaacgaa tcttgaagtt ttcaatttgg gaactggaag aggtttatcc gtactggaaa
     4801 ttatcaaaac ttttgaaaaa gtaaatggtg tgaaagttcc ttataaaatt gtaggacgcc
     4861 gaccgggaga tattgaaaaa gtttgggctg atccctctct ggcaaataag gaattaggtt
     4921 ggaaagctga aagaacgctg gaagagactt tgcgtagtgc atgggaatgg gaaaagaaat
     4981 tggcaagccg tgcaaaagaa aaagatcata ccaaaactga atcttaatca ctttttaaaa
     5041 taatgctata tccattaaaa tttcatccta ttttaaaaga aaaaatttgg ggaggcagtc
     5101 gtctaaaaaa attatttaat aagcctaccg agtccgacaa aattggtgaa agctgggaat
     5161 tatcaggtta tccaggtgat gaatccgttg tagcgaatgg ttttttagct ggcaatcagc
     5221 tcaatgaatt aatagaagtt tatatggacg aattggttgg tggcaaagta tttgatcgct
     5281 atggattaac cttccctctc ctttttaaat ttattgatgc caatgacgat ttgtctattc
     5341 aagtacatcc taatgatgaa acagctttaa aaaaacatga ctcattagga aaaactgaaa
     5401 tgtggtatgt ggtagatgcg gatgaaaacg gagagcttat agttggtttt aagaatgatt
     5461 gttcagcggc ggaattttta acagctttaa acgaaaaaaa ccttgaagat ttattacaac
     5521 gtgtttcggt aaaaaaagga gatgttattt ttattcatcc aggtttggta cacgcaataa
     5581 aaaaaggagt ggttgttgct gaaattcaag aaaatagtga tcttacttat cgtatttatg
     5641 actatcaacg gaaagacgaa aatgggaaag aaagggattt acatcttgaa gccgctcttg
     5701 aagtgattga tttcagtgca tgtaagaatc cactcgtaga ttatgtagaa aaaatgaatg
     5761 aagttaccac attggtttcc tgtcaatact ttactaccaa tttacttgcc tttaatcaaa
     5821 acttgttacg aaattatgtt caaatagatt catttgtagt ttatatgtgc ttggaaggaa
     5881 attttattat tgaatatgaa aatagccgaa caccagttga aaaaggagaa acagtcttga
     5941 ttcctgcttc aatggatgaa gttaatttaa ttcctgctga aggaaccgta aaattactgg
     6001 aagtgtatat agactgataa aaaacagcag aaaaattttc aagtttttct gctgttttct
     6061 gttaatcaat agttaagtga ttttattttt ctgtaacaaa taattttccc ggaacttttg
     6121 gaaaacaaaa atattttgaa aatcatctaa agtttttatc caatttttca aaataccatt
     6181 tttttcaaat ttttcctgtt caaaaatgtg aatgctgggt gtattttttt ctgctacaaa
     6241 agcatatatt tgattgattt ttaaataatc gaaaacatat tcttgaacca agcgaagact
     6301 atcagtagca tatccctttc tttgaaattt attttccaca tatattccca gagaaatacg
     6361 actattatga atatcaaaat cgaataagtc aatggttccg atggtttcat tattttcttt
     6421 taatacaatc atcaaacgca attgcttgtt ttcataaata tctttatcgg aatttaaaat
     6481 atattgtttc aacgtaaagt gagaataagg atttctcgca tttcccacca accacagttg
     6541 aggattgttt tcccatttat aaagtttttc cagatcctca ggttctactg ccctcaaacg
     6601 tatactctcg ttttctaata acaatttatt tttgctttat cgcttccttg atttttgctt
     6661 ccaattctgc tgccaattcg ggattatctt taatcaccgt ttttgcagca tctcttcctt
     6721 gtgctaattt ggtgtcattg taactaaacc aagaaccact ttttttaatg ataccatagt
     6781 caacgcctaa atccaaaatt tcaccggctt tagaaatacc ttctccaaac ataatgtcga
     6841 attcagcctt tcggaaagga ggagccactt tattttttac cacttttaca cgagtttgat
     6901 atccaattac ttcttcacca tcttttatag gagttactct acgtatatct aatcggacag
     6961 aagaatagaa ttttaaagca tttccccccg tagttgtttc tggattacca aacatgacgc
     7021 caattttttc acgcaactga ttgataaaaa tacacgtagt tttagtcttg ctaatgttgg
     7081 ctgtcaattt tcgcaaagct tgcgacatca agcgagcttg aagtcctaat ttagcatcac
     7141 ccatatcgcc ttccagttca gcttttggtg taagagctgc tactgaatcg atcacaataa
     7201 tatctaccgc tgaagaccga atcagttggt cggcaatttc gagggcttgt tccccattat
     7261 cgggttgcga aattaaaagt tcatcggtat ttacgcctaa attttctgca taagccctat
     7321 caaaagcatg ttcagcatca ataaaagcag caatgccacc cgctttttga gcttcggcaa
     7381 tagcgtgaat ggcaagtgtg gtttttccag aagattctgg tccgtatatt tcaataatcc
     7441 ttccacgtgg ataacctcct acaccaaggg caatgtctaa accaagtgaa ccagttggga
     7501 tcacatcaat atcaactact ttattgtcac ccattcgcat aatggttcct ttcccatagt
     7561 ctttttcaat tttgtccatt gccagttgca aagcttttaa tttttcggcg ttaggggaag
     7621 ttgtttttat ttcgtctgct gttttttctg ccatgataat tcagtttttt atttttaatt
     7681 gatgattgag gaaaatttta gaaaattatt gttttacaaa gataaaaatt ttttatttca
     7741 acacttctaa ttttgcaaat ttcagcaata aagatttttt tcctttttcg ccgaaatcta
     7801 tttcagctcg ttcattgcca tttacaatac aggtttcaat tacttttcca attccaaact
     7861 ggctgtgctt cactaaactg ccaacaggaa gagaaggttt ttcaataaga actttttctt
     7921 ccttcaaatc atttaacttt accagtcgtt tctgagagaa agaattttct actgttccct
     7981 gaccattaga ttgctggtaa aaatgtttcc gttcagttac taatgtatca tcccaacttg
     8041 tgctagtttg ttgggaaatc gtttcttcgc ttggcaaatc aagatattgt ttatcaatat
     8101 ctttgataaa tctactgggc tctgaaaaat aagtttttcc gtttctgaaa cgagattttg
     8161 catagctgat aaaacaatgt tctttagctc gtgtaatagc gacataaaat aaacgtcgtt
     8221 cttcttccaa ctcacgttca ttgaagatgc taaaaataga agggaacaat tgttcttcca
     8281 ttccaacaat aaaaactgct ctgaattcca atccttttgc agaatgaacg gtcattaaag
     8341 ttactctgtc tccttctgat ttatctgtat cctgatcagt taataaagaa atttctgata
     8401 aaaaatcagg taaaaagcct ttgtcttctt ccttttcttt cctttcacaa aattcatgga
     8461 gagctttcaa aagttcctga atattttcct gtttactaag attttcaggc gaattgtcag
     8521 aataaatatc ctgaaagatg ccagtgacat taatgagttg attgaccaaa tcataagcat
     8581 tttgtgtgtt tacttgtcct acaaaaacat taattaattc cttaaattgt tcaatttttg
     8641 ttaccgttct tgaattgatt ggtaatttat attctgccaa gtttgatatt acttcccaga
     8701 gacttatttg gttctgctga gcacattcga tgatcttgtt cagagtggtt tcgccaatgc
     8761 cacgagtagg ataattaata atacgtttta aagcttcttc gtcctgttga ttgcaaatca
     8821 atctgcaata agcaatgaca tcctttattt cttttctttc ataaaaagat aaacctccat
     8881 aaattcggta aggaatattt ctatttcgca aggcttcttc cagnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnntttgaaa atcttggcat cctggtaaga atttttaaat
     9061 tgaaaaatat tatcaatatt tgctccgcga aaagaataaa tactttgagc gtcatctcct
     9121 actacacaaa tatttttatg acgttcagcc agctttttta taattagata ttgtgcgaaa
     9181 ttggtatctt gatattcatc caccagaata tatcgaaaaa gttgctgata tttttgtaaa
     9241 acttcagggt gatcacgaaa cagcaaatta gtttgtagca ataaatcatc aaaatccata
     9301 gcattggctt gcttgcagcg aatacaataa gtctgataaa catcacataa tttacccaca
     9361 tgattttttc tatctttttc aattaagctg taatcagcag cataggcttt aggtgttatc
     9421 agattatttt ttgccatcga aatgcgggat tgaactaaac caggagtata gattttactg
     9481 tccagctcca tttcttttat aatgcttctt aatagacttt ttgagtcgga ttgatcgtaa
     9541 atgctaaaat tttgtgtaaa tccagtatat tgagcttcag cccgtagaat gcgtgaaaaa
     9601 atagaatgaa aagtacccat ccataaataa cgagcaattt tattcccaac aatttcagct
     9661 attcgttctt tcatttcacg agctgccttg ttggtgaaag tgagggccaa aatggaagaa
     9721 ggtggaaatc cttttttcag taaataagct actttgtaag ttagcacacg agtttttcct
     9781 gagccagcac ccgcaatcac cagcgaaggt ccatcagtat attctactgc cagacgttga
     9841 ctttcattga gttcatttaa aaaatcaaaa tttgtatctt tctctctttc aatcatttaa
     9901 aaatttatat tatgctgttt tggaagaaca aaaattacat taacttattg caaagttaat
     9961 aaacttaccc cacttttaaa tgtagaagga atagtttttc gtaaattttg aagagttaat
    10021 ttcttattat gactgagata taataatttt acaacttgaa tttttataaa gttggaatac
    10081 ttataataat ttttttatta cttttgatat aaagagtttg aaataatcaa atttaaaggc
    10141 aaaagacgat aggatgaaaa aattagtacg tttgattttc aaatattttg gctggcaaat
    10201 gatatatcct ttgcctgagc cacaaaagag cgtaatttgc ctggcacctc atacttctaa
    10261 ttgggatttt gtaattggtg aactgtctta ttggtccatc gggcaaaaag cttcttttct
    10321 tatcaaaaaa tcatggttca tttttccatt gaatttcttt ttcaaatcgt taggtggcat
    10381 tcctattgac cgttcaaaac acaattcgct tactcaacaa atgatcgata aatttcagaa
    10441 acttgacagt ttccatttgg tcattactcc cgaaggaact cgaggttatg taacagcatg
    10501 gaaaaaaggt ttttattata ttgctttggg agcaaatgtc cccattcagt tgtcttatat
    10561 tgattataaa aagaaggaat tgggtattac taaaattttt tatccaacag gaaatattga
    10621 tgctgatttt caagaaattc agaattttta tgaaagtgtt cagccaaaat atcctgagaa
    10681 atacaacaga aaaatttttt aatcatttgt ttatgaaagt tactttaata caagatgaaa
    10741 ttttttgggc agacaaaaca actaatttac gaaaagttgg tcaacaattg gaatttctct
    10801 caggaaaaac agatttggtt attttgcctg aaatgttttc aaccggcttt tgtaccgatc
    10861 aactaaattt agccgaaaca atggaaggcg aaacaataca gatgcttcgc tcttatgctt
    10921 taaaatacca attagccatc actggaagtt ttattgctac agaaaatggt aaattttata
    10981 atcgagcctt ttttattttt cctaccggtg aaatggtaac agccgataaa cgacatttgt
    11041 ttcaagcggg aggcgaaaat aaatttttta ctaaagggaa taaacgtctt caggtaagct
    11101 attatggttt taacatttgt gtgttggttt gttacgatgt tcgttttcct gtttggtcac
    11161 gaaatgtgaa caatgaatat gatttattga tttatgtggc caactttccc ttgaagcgaa
    11221 tcaatgattg ggacgtatta ttacaagctc gtgctattga aaatcaaact tatgtagcgg
    11281 gggtaaacag aattggtact gatggtcttg gaattgaata caatgggcat tcagctttac
    11341 tcgattttaa aggacgccct cttcttgctt ttcctgaaaa tgaattttct attcaaacaa
    11401 ccgaactgga taaagctcct ttaatttctt atagagaaag atttcccgtg tggaaagatg
    11461 cagatatttt tgaaataaaa aaataaatcc tttaattgta aaaagaattt tggtttttca
    11521 tcctttgttc tgttggattt gtaatctgat attttattta ttgtgaaaat caaacctcat
    11581 aaattattga aaattgcaaa ttctttccaa tagaaatttt tctccgtccc actttatatt
    11641 ttctcaatat agcttattga aaatgaatgg tttatcacac gaagggacat cgagattttt
    11701 tcatttcgta tgctgtacct caaaatgtgg gtttgggaaa ttttcggggc tacaaaaaaa
    11761 tcggtgctac gaaccttttt tgatatacat tacaggtaat aattctgaac ggcgttgcta
    11821 attacattca gagaaaagaa aaatgacctg ataaataagg taataaggtt gtaagctata
    11881 gggaacattc cttattatga aggttaaagg tttttgataa ggttttttta tgacaaatat
    11941 tggtttagca aaacgtttga ttttatcagc ggttaaaatc a
//
LOCUS       DAOSAS010000043        11973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3584_length_11973_cov_12.486827, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000043 DAOSAS010000000
VERSION     DAOSAS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11973
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3584_length_11973_cov_12.486827"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1178)
                     /locus_tag="PLR72_03185"
     CDS             complement(150..1178)
                     /locus_tag="PLR72_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005680314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOK36246.1"
                     /translation="MMNRKKIYSLIALLAIGLIIFFIFSKNKQGKQYIHNEGRIFGTY
                     YHITYQHPQQIDLQKKIEERLHEFENSLSIFNKQSIISRINQNDTSVVTDSYFEKMFQ
                     VAQKISKETNGAFDITVSPLVNAWGFGTNDTNRIQAPEVKNILPYVGYWKIHLENHKL
                     IKDDPRIMLDASAIAKGYSADVIGQLLKENQCENYLVEIGGEIVCKGLNPKGEKWRIG
                     IDKPIEDSTNMVNEIQTIIHISNKALATSGNYRQFYYKKERKFSHIIDPRTGFPVNNN
                     VLSTTVIAPTCIEADAYATAFMVLGVDSALELCKKLPEIDSYLIYVDNQGKDQVIYTE
                     GFKKYLSQ"
     gene            complement(1192..2541)
                     /locus_tag="PLR72_03190"
     CDS             complement(1192..2541)
                     /locus_tag="PLR72_03190"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOK36247.1"
                     /translation="MNKFQLIRNATIVNEGETFIGSVLIKDKFIQAIYANEADIQLKE
                     PFDEIDAEGYWLLPGVIDEHVHFREPGLTHKGDLYTESKAAVAGGVTSFMDMPNTIPP
                     TITIEALEQKFQLASEKSLANYSFYLGVTNDNFEELKKADIHRVCGIKIFLGSSTGNL
                     LVDNELTLERIFAEIPFLIAVHCEDEQMIQQHIRYYQNLIGEELPIFYHPLIRDTDVC
                     YRATTKAVKLAEKYQSRLHVLHVSTEKEISLFSSSIPLNEKKITAETCIHYLWFSDED
                     YEQLGNSIKCNPAIKSKDNRSALRKAINDNRLDVVATDHAPHLWNEKEGSCLKAASGA
                     PFVQHSLTAMLQLVKEGIFTLEKVVEKMCHAPAVIYKIQKRGFIRPGYYADLVLINPS
                     CSWQVSPDNLLCKCGWSSFTGTKFDSKVVKTWVNGHKVYDEGYFDESIKGEPLIFDN"
     gene            complement(2577..3317)
                     /locus_tag="PLR72_03195"
     CDS             complement(2577..3317)
                     /locus_tag="PLR72_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOK36248.1"
                     /translation="MPENLVIIPTYNEKENIENIIHAVFRQPYDFHILIVDDNSPDGT
                     AAIVKNLQKEFPEKLFLLERGGKLGLGTAYIEGFKWALDRDYKYIYEMDADFSHNPND
                     LPKLYQACANEGVDLAIGSRYLTGVNVVNWPLGRVLMSYMASIYVRLVTGLKITDTTA
                     GFKCYRREVLETIELDKIRFKGYGFQIEMKFKAYKSGFTLKEIPIIFINRELGTSKMS
                     GGIFSEAFLGVIKLKIQSFFYHYPQKKK"
     gene            complement(3356..4159)
                     /locus_tag="PLR72_03200"
     CDS             complement(3356..4159)
                     /locus_tag="PLR72_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK36249.1"
                     /translation="MAQLKFQSFGSGSSGNCYFIGNSNYGFLIDAGIGIRNIKKYLYN
                     IGLNFENILGVFITHDHADHIKSVGVLGEKYHIPIFTTKKVHEGIQKSFYVKKNLTFS
                     KKIIEKWESITIEDFCFTAFPVSHDATDSVGYVVIYQEKKFVFATDLGYISNEIITYL
                     TDANYLVLEANYDEKMLWDGSYPFYLKERIASDTGHLSNDQAGIFLSENYQEKLKNIF
                     LCHLSKENNLPTIAYNTVQKHLENNKIIVGKNVQLNVLERIQPSNIFIF"
     gene            complement(4323..4508)
                     /locus_tag="PLR72_03205"
     CDS             complement(4323..4508)
                     /locus_tag="PLR72_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOK36250.1"
                     /translation="MENEISIKVMKGGPYVISGNVKLILPSGEEKELVGKTALCRCGH
                     SKNKPFCDGSHHDVEFD"
     gene            complement(4618..6033)
                     /locus_tag="PLR72_03210"
     CDS             complement(4618..6033)
                     /locus_tag="PLR72_03210"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK36251.1"
                     /translation="MMINLQKKYRLFSILLLLVSFSVLGQTPQEADNLFQQKKYLEAK
                     KAYETLLRRNPKSALYNYRYARCCYELNDDENAIKYFERAGTKYPLRNKYLGELYMKA
                     YRFSESANAYQGYLTTLKENDADYDKIKLALKRAQLAENFIQRVDDISIIDSMIVDKS
                     KFLNFYNFTHELGSLSASPIQLKNRAEEKIVYTTERQDRMYFSDSIRGQMDIFTCYKL
                     LEKWSNPKPVSNQINTSANENYPFLMLDGLTLYFASDGENSIGGYDIFITRYVPSEND
                     FFIPDNIGMPYNSPYNDYMMVIDELHNIGWFATDRFLPEGKVTIFTFVPNETKKIIRS
                     NNLDSIREVAKLKTFRKVHRVDYHMEKENKENIFEPEKKFSFIINDTLIYTDVTQFRS
                     KESIQRWYELNKLNTDLQNKKMQLQELRLQYDQNENKKQELALKILALEKEITDQEDL
                     IDKKTIELRNCEIKFIQNNLH"
     gene            complement(6085..8784)
                     /locus_tag="PLR72_03215"
     CDS             complement(6085..8784)
                     /locus_tag="PLR72_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOK36252.1"
                     /translation="MKTSKWRKLWIILAVIALFFIILLCLPFVFKGKIVDFTKQQLNK
                     QLIAYIDFDKIQLSFIRRFPNASIQIKNLKIIGIDEFEKDTLLATEKIEAVVNLKSFF
                     SDTGYDISKILLANSQVKAHVLPDGKANWNIVKEKPEAEKDTSKMNFHLKLKDVILQN
                     INLTYLDEQGNMKIQVCNLNHHTSGDLTADSSLLKTDTRIDSLSFWMNNVAYLSKANV
                     KLKVDVDADLNNMMFKLSDNSILINALPFSINGWLKVLDDGFDMDLKLLADKVDFKSI
                     LSLIPALYYQSFKELKADGKISLDASVKGEMKEDNYPSFNLKLNVDDGWFRYPSLPKA
                     VQNIQIATQIFHPDGNLDKTVVDISKVSFVLDKQPFFVSCRILTPVSDPQIQAVAQGK
                     IEMDKIKDFYPFGDKTKLNGLLDVDASLNGRMSYIENNQYDKFNFQGKLNLERFLMKD
                     VNLYPQEITINNASLLMNPLFVNLSLSGLKIGENNLSAQGKLENLIAYLLKNKTLSGQ
                     LNAQSSYLNLNDFITSQPDNESATEKKVLEIPKNINFIVNADLNKVKFDKMIFENIKG
                     KLEIENGELKLQNVALQAFGGNILAKGLYSTLLPENPKLKVDLNIDSVVINKIFTQIG
                     FMEKLVPFLKNTDGKFSTNLSLGANLKNDMSLDLTSLNANGVFSTRAVGIKNSPIFNA
                     LANNLNRPEFMQPTLKDIALKFEILNGKIFIKPFDFKISNIKMNLGGSSSLNKEIDFQ
                     GIIQLPDQLNIGRFSTVKVNITGTYDKPKIKLDVMNTLSEIVDETKLKVEAEVAKQVD
                     KAKQEALEKAQKQKEKALQEAQQQADKIRLEAQKKSEKILEEARKEGDALVAKATNPV
                     AKKVAETTAKKLLDEAQKKVDQINSEAEAEAQKIIQNATKNLQ"
     gene            8976..9878
                     /locus_tag="PLR72_03220"
     CDS             8976..9878
                     /locus_tag="PLR72_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOK36253.1"
                     /translation="MSHHEHQDYQKHNDIKNIKTAFFLNFGFTIFEILGGIFTNSIAI
                     VSDAVHDLGDSLSLALAWYFQKKSKKSSDKSYSYGYKRFSLLGAIFNSIILLVGSFII
                     LYTAIPRLIHPEQPYVKGMFFFAIVGILVNGSALLQLKKGDSLNEKVVSLHMLEDVLG
                     WAAILIGTIIMYFWKVPIVDAILSIMIAVFILYNVLKNLRKSLHIILQGIPYEVDSEV
                     ISQYLLTQEEVESIHDLHIWSIDGTYNILTVHIVLKQSTPMSKIALLKKKYRSGLTSL
                     GIEHATIEFETSEEHCSMEQCVSE"
     gene            10080..10997
                     /locus_tag="PLR72_03225"
     CDS             10080..10997
                     /locus_tag="PLR72_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOK36254.1"
                     /translation="MENQERTAQAIHVTWVGFWTNFILTIFKLLAGFFGKSTAMIADG
                     VHSLSDFITDVIVIVFIGISGKERDKDHQYGHGKFETFASFLVSLALVVVGVFIFLNG
                     LTKVIQIIKGNVIEPPSMIAFVAALVSIVTKEWVYRYTVKTGKKIDNQAVIANAWHHR
                     SDAFSSLGTGIGIGGAIFFGEQARILDPIAGIIVSFFIVQVAIKILVPSVNELLERSL
                     PEETEKEIFQILETNPEILSYHNLKTRKIGNIFAIDVHIKLDPSTTFVRSHEVATDIE
                     NKLRNRFGKQTIINIHTEPFNEEKDKKMK"
     gene            complement(11242..11325)
                     /locus_tag="PLR72_03230"
     tRNA            complement(11242..11325)
                     /locus_tag="PLR72_03230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11289..11291),aa:Leu,seq:gag)
     gene            complement(11354..11437)
                     /locus_tag="PLR72_03235"
     tRNA            complement(11354..11437)
                     /locus_tag="PLR72_03235"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11401..11403),aa:Leu,seq:cag)
     gene            11700..>11973
                     /locus_tag="PLR72_03240"
     CDS             11700..>11973
                     /locus_tag="PLR72_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36255.1"
                     /translation="MKRAFLGLSINTYEQNHANSFQLLKINFIRMKNIWFFIILFFEI
                     IGNLQVFAVPAYPYPIKVTQPDGSDITFIMKGDEFYHYHTTLDGYPI"
BASE COUNT         4035 a         1946 c         1665 g         4327 t
ORIGIN      
        1 aaaaaaaatt gaacttcaaa attaactatt ttattgtaaa caaaaaagtt aaattttcat
       61 atttgagtaa aattcctttt ctgctttaga taaggtatta caaaattaac gaaaatagct
      121 ctcaaatttt aaaaaataat ctatcttttt tattgagaaa gatatttctt aaacccttca
      181 gtataaatta cttgatcttt tccttgattg tccacatata tcaagtaact gtctatttca
      241 ggtaattttt tacaaagttc caaagcacta tccacaccta ataccataaa agcagtggca
      301 taagcatctg cttcaatgca ggttggtgct atcacagtgg tgcttagcac attgttattc
      361 actggaaaac ctgttcttgg atcgataata tgagagaatt ttctttcttt tttgtaataa
      421 aattggcggt aattgcctga agtcgccaaa gctttattgg aaatatgaat aatggtttga
      481 atttcgttta ccatgtttgt actatcctca attggtttat caattccaat acgccatttt
      541 tcacctttag gattcagtcc tttacaaact atttcacctc caatttctac cagataattc
      601 tcacattgat tttcttttaa taattgacca attacatcag cagaataacc tttggctatg
      661 gcactcgcat ccagcataat tcgaggatca tcctttatta atttatgatt ttccagatga
      721 attttccaat aaccaacata tggcaaaata ttctttacct caggtgcttg aattcggttg
      781 gtatcatttg ttccaaatcc ccatgcattt actaaaggtg atacagtaat atcaaaagct
      841 ccgttagttt cttttgaaat tttttgagct acctgaaaca ttttttcaaa ataggaatca
      901 gtaactaccg aagtatcatt ttgattgata cgggaaataa tagattgttt attgaaaatg
      961 gaaagtgaat tttcaaattc atgtagccgc tcttctattt ttttctgtaa atcaatttgt
     1021 tgtggatgtt ggtaagtaat atgataataa gttccaaaaa tccttccttc attatgaata
     1081 tattgttttc cctgcttgtt tttgctaaag atgaaaaata ttattaatcc gatagccaat
     1141 agggcaatta aagaatagat tttttttctg ttcatcataa atacactgtt ttcaattatc
     1201 aaaaattaac ggttcgcctt ttatactttc gtcaaaatat ccttcgtcat aaactttgtg
     1261 accattgacc caggttttaa caacttttga atcaaattta gttcctgtaa aagatgacca
     1321 gccacacttg catagcaaat tatcaggcga aacttgccag gaacacgatg gattaatcag
     1381 cactaaatca gcataataac ctggtctgat aaaaccacgt ttttgaattt tataaataac
     1441 agcaggagca tggcacattt tttctactac tttttccagt gtaaaaatac cttcttttac
     1501 caattgtaac atagcggtta gcgaatgttg tacaaacggt gctccagaag cagcttttaa
     1561 acaagaacct tccttttcgt tccacaaatg aggtgcatga tcagtcgcca ccacatccaa
     1621 tcgattatca tttatagctt ttctcaatgc tgaacgatta tctttgcttt taatagcagg
     1681 attacatttg atactatttc ctaattgttc ataatcctca tcagaaaacc ataaataatg
     1741 aatgcaggtt tctgctgtta ttttcttctc attcaatgga atactggaag agaacaatga
     1801 tatttccttt tcagtagaaa catgcaacac atgcaaacga ctttgatatt tttctgctaa
     1861 ttttacagct ttcgttgtag ctcgataaca aacatctgta tctcgaatta atggatgata
     1921 aaaaataggg agctcctctc ctatcaaatt ttgataataa cggatatgtt gttgaatcat
     1981 ttgttcatct tcacaatgaa cagcaatcag aaagggaatt tcagcaaaaa ttcgttctaa
     2041 agtcaattca ttgtcaacca gcaaatttcc tgttgatgac cccaaaaaaa ttttgattcc
     2101 acaaacacga tgaatgtctg cttttttaag ttcttcgaaa ttatcattgg taactccaag
     2161 ataaaaagaa taatttgcaa gtgatttttc tgatgcaagt tgaaattttt gctctaaagc
     2221 ctcaatggtt attgtgggtg gaatagtgtt aggcatatcc ataaaactgg taacaccacc
     2281 tgctaccgca gccttgcttt cggtatataa atcgccttta tgagttaagc ccggttcacg
     2341 aaaatgaacg tgttcatcaa ttacaccagg caaaagccaa taaccttctg catcgatttc
     2401 atcgaaaggt tctttcaatt gaatatctgc ttcattcgca tatattgctt gaataaattt
     2461 atcttttata agtaccgaac ctataaaagt ttctccttca ttaacaatag tcgcatttcg
     2521 gattaattga aatttattca taaattataa acattgcttg gggaaggatt cgaaaattat
     2581 tttttctttt gtggataatg ataaaagaaa ctttgaattt ttaatttgat cacccctaaa
     2641 aaagcctcac taaaaattcc gccactcatt ttggaagtac ctaattctct atttataaag
     2701 ataatgggta tttcttttaa tgtaaaacca cttttataag ctttgaattt catttcgatc
     2761 tggaaaccat aacctttgaa acgaatttta tccagttcaa ttgtttcaag cacttctcta
     2821 cgataacatt taaaacctgc agtagtatct gtaattttca gacctgttac taatcttaca
     2881 taaatagaag ccatataaga cattaatact cttcctaaag gccagttaac tacatttaca
     2941 ccagttaaat aacgagaacc aattgctaaa tcaactcctt cattagcaca agcttgatat
     3001 aatttcggta aatcatttgg attatgcgaa aaatcggcat ccatttcata aatatattta
     3061 taatctctat ctaatgccca tttaaaaccc tctatataag cggttccaag tcctaatttt
     3121 cctcctcgtt cgagaagaaa aagtttttca ggaaattctt tttgtagatt ttttacaatt
     3181 gcggctgtcc catcgggtga attatcatca acaattaata tatgaaaatc ataaggttga
     3241 cgaaaaacag catggataat attttcaata ttttcctttt cgttataggt tgggataata
     3301 actaaatttt caggcataaa ttattaattt caacttgcga agaatcattg attgattaaa
     3361 aaataaatat attcgatggt tgaattcttt ctaaaacatt taattgaaca ttttttccaa
     3421 cgataatttt attattttct aaatgctttt gaacagtgtt atatgctatt gttggtagat
     3481 tattttcttt actgagatga cacaaaaaaa tatttttgag cttttcttga taattttcgg
     3541 agagaaaaat tcccgcttga tcattgctca aatgtcctgt atcagaagca attcgttctt
     3601 ttaagtaaaa aggatacgaa ccatcccata acattttttc atcataattt gcttctaata
     3661 ccaaataatt tgcatcagtt aaataagtta taatttcgtt gctaatgtaa cccaaatcag
     3721 tagcaaaaac aaattttttc tcttgataaa ttacgacata acctacacta tcagttgcgt
     3781 catgagaaac aggaaaagcc gtaaaacaaa aatcttctat ggttatactc tcccattttt
     3841 caatgatttt tttgctgaaa gtcaaatttt tctttacata aaagcttttt tggatacctt
     3901 cgtgaacttt tttcgtcgta aaaataggta tatgatattt ttcacctaat acgcctactg
     3961 atttgatatg gtcagcatga tcgtgagtaa taaaaacacc taaaatattt tcaaaattaa
     4021 gtccaatatt gtacagatat ttttttatgt ttcgtatgcc tattcctgca tcaattaaaa
     4081 atccatagtt tgaattgcct ataaaataac aatttccact gcttccactt ccaaaacttt
     4141 gaaactttaa ctgagccatc acttttttga aggcaaaaat aaaagaaaaa agttgaatta
     4201 agtgcacaaa attttagaaa attaaattaa gtgataataa aatgaggcat aaaatataaa
     4261 aaaacgaaac agtaataaat agtttttact gtttcgtcag gataaaaatt ttacattaac
     4321 aattaatcaa attctacatc atgatgagaa ccatcgcaaa aaggcttatt tttactgtgt
     4381 ccacatctgc ataaagcagt ttttccaact aattcttttt cctcgcccga tggcaaaatt
     4441 aatttaacat taccacttat aacatacgga ccgcctttca ttacttttat tgaaatttca
     4501 ttttccatga ttttttgttt ttaaatgtta tttacataaa aacaatttca acaataaaaa
     4561 gtttcaggta aaattaacat tattaaaaca ttaatgttaa aaattgaaaa taaaatttta
     4621 atgtaaatta ttttgaataa attttatctc acaattacgc aactctatgg tttttttgtc
     4681 tattaagtct tcctgatcgg taatttcttt ttccaatgct aatattttca gggcaagttc
     4741 ttgcttttta ttttcatttt gatcatattg caatcgcaat tcctgcaact gcattttctt
     4801 attctgcaag tcggtattta atttgtttag ttcgtaccaa cgttgtatgg attctttact
     4861 tcgaaattgc gtgacatctg tataaattaa agtatcattt attataaaac taaatttttt
     4921 ttcaggctca aaaatatttt ctttattctc tttttccatg tgataatcaa cacgatgtac
     4981 cttcctaaat gtttttagtt ttgctacttc tcgaattgaa tctaaattat tagatcgaat
     5041 aatttttttt gtttcattag gaacaaaggt aaaaatagta actttccctt cgggcaaaaa
     5101 acgatcggta gcaaaccaac ctatattatg caattcatca atcaccatca tatagtcatt
     5161 ataaggcgaa ttgtaaggca ttccgatatt gtcaggaatg aaaaaatcgt tttctgatgg
     5221 aacataacgg gtaataaaaa tgtcatatcc tcctatggaa ttttcaccat cagatgcaaa
     5281 atacagggta agtccatcca acataaggaa aggataattt tcattggctg aagtattaat
     5341 ttgattggaa acaggcttag gattagacca cttttctaat aatttgtaac aagtaaaaat
     5401 atccatttgc ccacgaatag aatcagaaaa atacattctg tcttgacgtt cagtagtata
     5461 aactattttt tcttccgctc gattttttaa ttgaatggga ctggcactaa gggaacctaa
     5521 ttcatgagta aaattataaa aatttagaaa tttactttta tctactatca tactatctat
     5581 tattgaaata tcatctactc tttgaataaa attttcagcc aattgtgctc ttttcaatgc
     5641 aagtttgatt ttatcataat ctgcatcatt ttcttttaag gtagtaaggt atccttgata
     5701 agcatttgca gattctgaaa aacgataagc tttcatgtac aattcaccta aatatttgtt
     5761 tcgtaaagga tattttgttc cagcacgttc aaaatatttg atagcatttt catcatcatt
     5821 tagttcataa cagcaacgtg cataacgata attatataaa gcactttttg gatttcgtcg
     5881 taacaaagtt tcataagctt tttttgcttc gagatatttt ttttgctgaa acaaattatc
     5941 tgcctcctgg ggtgtttgtc ccaatactga aaaggaaaca agtaataata atatagaaaa
     6001 taatcggtat tttttttgta agttaatcat catttaaaaa tcgtttaaca actattttaa
     6061 aaacgaattc agttaaaaat ggatttattg caaatttttt gtcgcatttt ggataatttt
     6121 ttgggcttca gcttctgctt cactgtttat ttgatcaact tttttctggg cttcgtccaa
     6181 taattttttt gcagtagttt cagctacttt tttagccaca ggatttgtag cctttgcaac
     6241 aagggcatcg ccttctttac gagcctcttc aagaattttt tcactttttt tctgagcctc
     6301 taatcgaatt ttatcagctt gttgttgagc ttcctgtaaa gctttttctt tttgtttttg
     6361 agccttttct aatgcttctt gtttggcttt atctacttgt tttgctactt cggcttctac
     6421 ttttaatttt gtttcatcta ctatttcact taaagtattc atgacatcca acttaatttt
     6481 tggtttatca taagtacctg taatgttgac ttttacagta gaaaaacgtc ctatgttcag
     6541 ttgatcaggt aattggataa ttccctgaaa atcaatttct ttgtttagac tcgaactacc
     6601 acctaaattc atttttatat ttgaaatttt aaaatcaaaa ggttttataa agattttacc
     6661 gttcaatatt tcaaatttta aagcaatatc ttttaaagtt ggttgcataa attcaggacg
     6721 attcaaatta ttagccaaag cattgaaaat aggagaattt tttattccaa ctgctctggt
     6781 tgaaaaaact ccattcgcat ttaatgaggt taaatctaaa ctcatatcat tctttaaatt
     6841 tgcaccaagc gaaagattcg ttgaaaattt tccatcagta tttttcaaaa atggaaccag
     6901 tttttccata aatcctattt gagtaaaaat tttattaata acaacactat caatgtttaa
     6961 atctactttt aattttggat tttctggcaa taaagtactg tacaaacctt ttgctaagat
     7021 atttccacca aaagcctgca atgcaacatt ttgaagtttc aattctccat tttcaatttc
     7081 taatttccct ttaatgtttt caaaaatcat tttatcaaac ttcactttat ttaagtcagc
     7141 atttacaata aaatttatat ttttgggaat ttccaaaact ttcttttctg ttgcactttc
     7201 gttatcgggc tgtgatgtaa taaaatcatt taaattgaga taagaagact gtgcatttaa
     7261 ttgaccactt aaagttttgt tttttaataa gtaagctatc aggttttcaa gctttccttg
     7321 agctgataaa ttgttttcac ctatttttag ccctgataat gataaattta caaataaagg
     7381 attcatcaat aaactcgcat tattaatggt gatttcctgt ggataaagat ttacatcttt
     7441 cattaaaaac ctttccaaat ttagtttccc ttgaaaatta aatttatcat attgattatt
     7501 ttcaatatac gacattcgtc catttaaact ggcatctaca tcaagaagtc cattcaattt
     7561 ggttttatca ccaaatggat aaaaatcttt aattttatcc atttcaattt ttccttgagc
     7621 aactgcctga atttgtggat cagaaacagg ggttaaaatt cggcacgaaa caaaaaaggg
     7681 ttgtttatct aatacaaatg aaaccttaga aatatccacc actgttttat ctaaattacc
     7741 atcaggatgg aatatttgag tagctatttg gatattctga acagctttag gtaaagaagg
     7801 ataacgaaac cagccatcat ctacatttaa ttttaagtta aaagaaggat aattatcttc
     7861 tttcatttcc ccttttactg aagcatcgag agaaatttta ccatccgctt ttaattcttt
     7921 gaatgattga taatacaaag ctggtatcaa tgacaaaatc gatttaaaat caactttatc
     7981 ggctagcaat tttaaatcca tatcaaatcc atcatccaaa actttcaacc aaccatttat
     8041 agaaaaagga agagcattga tgagaattga attatcactc aatttaaaca tcatgttatt
     8101 caaatcagca tcaacatcta cttttaattt tacattagct tttgaaagat atgccacatt
     8161 gttcatccaa aaactcaaag aatcaattct ggtatcggtt ttcaacaatg agctgtcagc
     8221 agtcaaatca ccactggtat gatgattcaa attgcagact tgaattttca tatttccttg
     8281 ttcatcaaga taagtcaaat ttatattttg caaaatgaca tcttttaatt ttaaatgaaa
     8341 attcattttt gaagtatctt tttcagcttc tggtttttct ttaactatat tccaatttgc
     8401 ttttccatca ggtaaaacat gagcttttac ttgagaattg gccaaaagaa tttttgaaat
     8461 atcgtaacct gtatcggaaa aaaaactttt taaattcact actgcttcaa ttttttcagt
     8521 agccaataaa gtatcctttt caaattcatc tattccaatg attttcaagt ttttaatttg
     8581 tatagaagca ttggggaaac gacgtatgaa acttagctga attttatcga aatcaatata
     8641 agcaattaat tgcttgttta attgttgctt tgtaaaatca acaattttac ctttaaaaac
     8701 aaaaggcaaa cacaataata ttataaaaaa caatgcaata actgctaaaa taatccacag
     8761 tttacgccat ttagatgttt tcatattttt ctgaatttaa aatgattgat ttatttttca
     8821 aagataggat taatttaaca aaaaatagaa taattaagtg atgctttttc aatcaattta
     8881 atcaaaaaac atacctttgt attttaaaaa agtaaacatt ttaactaaat tttaaaagtt
     8941 ttttcgtaat catttttcca aaaacataac taatcatgtc gcatcacgaa catcaagatt
     9001 atcaaaaaca taacgatata aaaaatatta agacagcttt ttttcttaat tttggattta
     9061 ccatttttga aatattggga ggtattttta ctaacagtat tgccattgtt tcagatgccg
     9121 tacatgattt aggcgacagt ctttcgctgg ctttggcatg gtattttcag aagaaatcca
     9181 aaaaaagcag tgacaaatct tattcatacg gttacaaacg attttcgttg ctgggagcaa
     9241 tttttaattc tatcattctt ttagtaggaa gttttattat tctttatact gctattccac
     9301 gtttgattca tcctgaacaa ccttatgtaa aaggaatgtt tttctttgcc attgtaggaa
     9361 ttttggtaaa cgggagtgct cttcttcagt tgaaaaaagg tgattcgtta aatgaaaaag
     9421 tagtttcact tcacatgcta gaagatgtac ttggctgggc agctatttta attggcacca
     9481 ttattatgta tttctggaaa gttcctattg ttgatgccat tctttccatt atgattgctg
     9541 tttttatttt atacaatgtt ttgaaaaatt tgcgaaaaag tttgcatatc attttacaag
     9601 gaattcctta tgaagtagat tccgaagtca tcagtcaata tcttttgact caagaagaag
     9661 tggaaagcat tcacgatttg cacatatggt ccattgatgg cacttacaac atacttaccg
     9721 ttcatattgt tttaaaacaa tccacgccca tgagcaagat agccctgtta aaaaaaaagt
     9781 atcgcagtgg acttacatcg cttggcattg agcacgccac cattgaattt gaaacctccg
     9841 aagagcattg ttcaatggag caatgtgtaa gtgaataatt tagaatgatt taaaacatta
     9901 tttattctct ttataaatta gcttctcaaa acaaaataat taaggaaatg tctttttaat
     9961 gattattttt gtaagattaa gcaaaaataa ttgtgacaca agctgagtaa aataatttta
    10021 tgcttcagca aaatatttta ttagcaaaat ccatatagtc ttattaatat accaagtgta
    10081 tggaaaatca agagagaaca gcacaagcta ttcatgttac gtgggtaggt ttttggacaa
    10141 atttcatttt aactattttt aaactattag caggtttttt cggcaaaagt actgccatga
    10201 ttgctgatgg tgtgcattcg ttgtctgatt ttataaccga tgtaattgtt attgtattta
    10261 ttggaatttc aggaaaagaa cgcgataaag accatcaata tggacatgga aaatttgaaa
    10321 cgtttgcttc atttctggta agtttagcat tggtagtagt aggagtattt atttttttga
    10381 atggacttac aaaagtaatt caaattatta aagggaatgt aatagaaccg ccttcgatga
    10441 tagcttttgt agctgcttta gtaagtatcg tgacaaaaga atgggtatat cgttatacag
    10501 taaaaacagg aaagaaaatt gataatcaag ctgttatagc aaatgcttgg catcatcgct
    10561 cggatgcatt ttcttccctt ggtacaggta taggtatcgg gggagcaatt tttttcgggg
    10621 aacaagctcg gatattagat cccatagctg gaattattgt gagctttttt attgttcaag
    10681 tggcaattaa aattttagta cccagtgtaa atgaattact tgaacgttca cttccagaag
    10741 aaaccgaaaa agaaattttt caaattctcg aaacaaatcc tgaaatatta tcatatcata
    10801 atttaaaaac ccgtaaaatt ggaaatatat ttgccattga tgttcatatc aaattagatc
    10861 cttccactac ttttgtacgt tcgcacgagg ttgccacaga cattgaaaat aaacttcgaa
    10921 atcgttttgg gaaacaaacc attatcaaca ttcatacaga accttttaat gaagaaaaag
    10981 ataaaaaaat gaaataaatt tgacaggatt tattagaata aagaaaatta ttctttttaa
    11041 ctgttcggca agacgttata aaaagcagta taagcataca aattcaaaga aaacaatccc
    11101 cgcatttaat aagataataa agttggaaga aaaaaatttt cggcgttatg aaggttatca
    11161 agttttattc acgtttttat tcattatagt ataaaacttg aacttggata tgctgaaaat
    11221 caaataaata gaaaattatt gtgcgcagaa tgagactcga actcacacgc cgaagccggc
    11281 actaccccct caaagtagcg cgtctaccta ttccgccatc tgcgcaaaat tttaaagata
    11341 atcttcaatt ttgtgcccaa aacaggactc gaacctgcac agccttgcag ctactagtcc
    11401 ctgaaactag cgcgtctacc aattccgcca tttgggctta aaaatttttt cttcaatatc
    11461 tctgtttcta aaaaggatag agttctattc aaaaggcatt attaaaaaac ttagcaaaga
    11521 tacatttttt taccaattgc caaaaacttt aaaagtaaaa aattgttgtt taaaggtagc
    11581 taacaaattt ataattatat ttgtacatat aattttaatt attctttatc atccgaaaat
    11641 tattgtatta atttttataa attgttgatt atcacaataa aacaagttat tttaaaaata
    11701 tgaaacgagc atttttagga ctaagcataa acacgtatga acaaaatcat gcgaattctt
    11761 tccaactttt gaaaattaat tttattcgga tgaaaaatat atggttcttt attattctgt
    11821 tttttgaaat aataggcaat ttgcaagttt ttgcagtacc agcctacccc taccccatta
    11881 aagttaccca gccagacgga agtgacataa ccttcataat gaaaggagat gaattttatc
    11941 actatcatac tacattggac ggatatccca taa
//
LOCUS       DAOSAS010000044        11924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3607_length_11924_cov_11.890302, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000044 DAOSAS010000000
VERSION     DAOSAS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3607_length_11924_cov_11.890302"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..517
                     /locus_tag="PLR72_03245"
     CDS             131..517
                     /locus_tag="PLR72_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36256.1"
                     /translation="MDAIGAPGATVPTTYVTSGKLTGMSNVYFNKNLAFSSAKQLFFT
                     MGHEFVHVSQFAALAGQRASILTQDFIDMLDFHAYSYQNSIGGLQLNSYTREEILHWS
                     TTYSQFNSMNYINFPWSFNHSYIYPF"
     gene            519..1328
                     /locus_tag="PLR72_03250"
     CDS             519..1328
                     /locus_tag="PLR72_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36257.1"
                     /translation="MKAIIFYISLSMLLSIYATTYSQCISIELSARWVKGYDVFQKDS
                     IICYPELIITYRNMSDTNYYFQKVSESRIGLPMLPIGHLLQYPIEEYLNPDYLKRAKM
                     YGKYSGENYTVIFGGSSFSKGWIVVNDTSNIEVEQEIDLINDDLANIYEYIYRENYDK
                     QRDSLNEYNTHYTLSDTVPKLILDKFKDKFVFLRPGEIYTDTYNLVGFKLVKGCFTFN
                     IIPYLPAYVYTTIWDKSQSKYIEVKIELPKVVGNYKLYSGSFNSNKIAITF"
     gene            1491..1736
                     /locus_tag="PLR72_03255"
     CDS             1491..1736
                     /locus_tag="PLR72_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36258.1"
                     /translation="MIKPNISPSKNNIRYYFGTYEKDIDKFGNFTQTDYIYTPAGLTA
                     MVKNGTLYYVHTDNLVSIQAITDENKNIVSSYYYSLI"
     gene            complement(1816..2061)
                     /locus_tag="PLR72_03260"
     CDS             complement(1816..2061)
                     /locus_tag="PLR72_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HOK36259.1"
                     /translation="MGHPMQYLFNLEKGRILQEYQIIYITQRYGVFQNKHKVYRIYPG
                     SVIILFPEEWHRYRPLKTLDEKVSTSDLMVIFSSVTS"
     gene            complement(2469..4076)
                     /locus_tag="PLR72_03265"
     CDS             complement(2469..4076)
                     /locus_tag="PLR72_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOK36260.1"
                     /translation="MNKNKLLAPFIVFVAIICGLLLGNILARRASNLSVDTLFSAIKP
                     HSKIDELFSIINSKYVDTLDMHQLTEATMVDIVSKLDPHSVYISAEDVSDVNSELEGH
                     FSGIGVQFNIQNDTIMIISVISGGPSEKAGLLAGDRIVEVNDTLFVGKNITNEKVMKK
                     LRGPAGTKVKVGVRRYGTPEILYYEIIRGEIPVNSVDIGYMIQPQIGFIRVSKFAETT
                     YTEFLNTIASLKRQGAKKFIVDLRENSGGYMDQTIQMVNEFLPANRLIVYAQGRAYKR
                     FEAFSDGKGSCIHEPLVVLINEFSASASEIFAGAIQDNDRGTIVGRRSFGKGLVQQQF
                     TLSDGSAVRLTVARYYTPSGRCIQKPYELGKVDQYEKDFLNRLIHPDINYSDSTQKID
                     SLRYKTVGGRIVYGGGGIMPDVYVPLDTTGYTPYLNKVINYGYLYRYAFLYTDQNRGK
                     LSAFKNWQQMEKYLDSQPLLENFIHFALMKGVPANKKEINISKDIILTQLKAYISRNI
                     LGDEGFYPLLYKNDKTVRKALEVLSRQ"
     gene            complement(4155..4574)
                     /locus_tag="PLR72_03270"
     CDS             complement(4155..4574)
                     /locus_tag="PLR72_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOK36261.1"
                     /translation="MTEKQRLLDQRYMRMARIWGENSYCKRRQVGALLVKDKMIISDG
                     YNGTPSGFENDCEDEENNSKPYVLHAEANAITKVARSHNSSEGATLYVTASPCMECAK
                     LIIQAGIKRVVYGEQYRIADGIELLKRAGIEVVFIDV"
     gene            complement(4610..5977)
                     /locus_tag="PLR72_03275"
     CDS             complement(4610..5977)
                     /locus_tag="PLR72_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOK36262.1"
                     /translation="MQRVHFIAIGGAAMHNLAIALSKKPDYFVTGSDDEIFDPSLSHL
                     KEHGLLPEQLGWFPERIHKGLSAVIVGMHAKADNPELLRAKELGLKIYSFPEYLYYQT
                     RSKTRIVVGGSHGKTTTTAMILYVLQKLKINADYMVGAQIEGFDNMVKLSYESRIAVF
                     EGDEYSTSPLDPRPKFHLYQPHIAILTGIAWDHVNVFPTFEEYVNQFKKFTDLIEFQG
                     RLIYFEGDDNLNKIVQHLRPDIVTFPYNTPPYEVRDGITYLITKKREVPLKIFGEHNL
                     QNLTAARIACHQIGVNDEDFYTIISDFTGASNRLQKIAEKNNTVVFKDFAHSPSKLKA
                     TIKAVRQQYPDKKLIACMELHTFSSLTEDFLPQYYGSMSEADIALVYYDPEVIKQKQL
                     QEFSPTDVKNAFGEKVEAFNRKEDLLEKLHELNYENTVVLFMSSGNFSGINLEELTNE
                     LIKNQ"
     gene            6076..6915
                     /locus_tag="PLR72_03280"
     CDS             6076..6915
                     /locus_tag="PLR72_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK36263.1"
                     /translation="MKIYKIEAGTFHCDGGAIFGVVPKKVWQKRYPCNEENFCTLSMR
                     CLLIDTGNKLIIIDSGTGNKQLEYLKYYDFKGVVNFEKELNKLGYSCNEVTDVILTHL
                     HFDHCGSCTYYNQNRTEIMMTFPNATHWVGKTQWENFLHPNVREGDSYFLENMQPVYE
                     AGKLQLVENNFQFCPEIEIRIFNGHTVGQLASYIQYNDKTLIYVGDVIPVAPAIPLAW
                     VSAFDTYPVTSMEEKQLLLEEAVEKEQILFFEHDAYTECCTVKRNNDNYRMDKSFPLS
                     NIQ"
     gene            complement(6953..7498)
                     /locus_tag="PLR72_03285"
     CDS             complement(6953..7498)
                     /locus_tag="PLR72_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOK36264.1"
                     /translation="MAHIQSVRGFTPKIGKNCFLAPTSVIIGDVIMGDGCSIWFNAVL
                     RGDVNSIRLGNHVNVQDGSVLHTLYQKSTIEIGNYVSIGHNVTIHGAKIHDYALIGMG
                     AVVLDDAEIGEGAIIAAGALILSNTKVPPYTMWAGVPAKFVKKVDPSQTNEMNRKIAH
                     NYSFYASWYQEDNDSKRQNEN"
     gene            7819..9549
                     /gene="lysS"
                     /locus_tag="PLR72_03290"
     CDS             7819..9549
                     /gene="lysS"
                     /locus_tag="PLR72_03290"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOK36265.1"
                     /translation="MSTELSDQEQYRRQSLIELRKLGIEPYPAALYPTNAFSVDIKNS
                     FKDSEEKKQVCVAGRIMSRRIMGKASFIELQDAKGRIQVYITRDDISTEEQPEMYNTV
                     FKKLLDIGDFIGIKGFVFRTQMGEITIHAQELTVLSKSLRPLPIVKYKDGVAYDAFND
                     PEQRYRQRYVDLVVNESVKDIFLKRTQIFQSMRDYFNAQGYVEVETPILQPIPGGAAA
                     RPFVTYHNALDMQLYLRIADELYLKRLIVGGFEGVYEFSKNFRNEGMDRTHNPEFTAM
                     EIYVAYKDYFWMMEFTERLLEKICLDVNNSTEIHLENKVIDFKAPYKRIKMLDVIKEN
                     TGIEIDGMDENQLREVCRQLDIEVDETMGKGKLIDKIFSEKCEANLVQPTFIIDYPIE
                     MSPLCKRHRNNPELTERFELIVNGQELANAYSELNDPIDQLERFKEQLRLSEKGDEEA
                     MFIDMDFIRALEYGMPPTSGLGIGMDRLVMLLTGQQAIQEVLLFPQMKPEKVIKSDDK
                     EKFLHLGIKEPWLDVMEKIGIKTIEQLKVFNPNKLHQDLCSWNKKNKLELVNPTIEEV
                     NNWISKAKDN"
     gene            9594..10580
                     /locus_tag="PLR72_03295"
     CDS             9594..10580
                     /locus_tag="PLR72_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOK36266.1"
                     /translation="MFELIFELSSHVDTTIFYGINNVNIQLIKNLYPNLKIIARGHII
                     KIMGSEEEADKLFLNLQKLEQYSIENNKLTEKNIIDLIKDNVPTDIVKYENLILHGSG
                     GKSIIARTKNQQKLVKEFETNDLLFAIGPAGSGKTYTAIALAVRSLKNKEVKRIILSR
                     PAVEAGEKLGFLPGDMKEKIDPYLQPLYDALQDMITPLKLKEYLEDGTIQIAPLAFMR
                     GRTLNDAVVILDEAQNSTIMQIKMFLTRMGINTKMIVTGDITQIDLPRDQKSGLIDAL
                     QLLKNIPGIARVDFDVHDIVRHKLVQEIVQAYEKRNNLMQTKSKKEDNETEK"
     gene            10615..11562
                     /locus_tag="PLR72_03300"
     CDS             10615..11562
                     /locus_tag="PLR72_03300"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOK36267.1"
                     /translation="MNTLIRTNFHFEGQTNVYYGKVRDVYTIKNDLLIMVATDRISAF
                     DVILPRGIPYKGQILNQIAAKFLDETADIVPNWKLACPDPMVTVGLRCEGYPVEMIVR
                     GYLCGSAWRAYKNGARELCGVKIPDGMRENQRFETPIITPTTKAEHGLHDEDISKEEI
                     LQKGLMTPDEYELIEKYALALFQRGTEIAAQRGLILVDTKYEFGKRDGKIYLIDEIHT
                     PDSSRYFYLEGYQERFEKGEPQKQLSKEFVREWLMENGFQGKAGQTIPEMTDEIVKSI
                     SDRYIELYEQITGEPFVRTDTENLEQRIEKNILDYLKNH"
BASE COUNT         4114 a         1974 c         1992 g         3844 t
ORIGIN      
        1 attaatgcaa tacaatatct tgtaaataaa taatttataa gcatattttt ctattagtgt
       61 ccattagaaa atactgacta aaaaaataat cctaatatca tattgttcta atggacaatt
      121 tgggatgaaa atggatgcaa ttggtgcacc tggcgcgaca gtgccaacta cttatgttac
      181 aagcgggaaa ttaacaggta tgtcaaatgt atatttcaac aaaaacttag cattcagcag
      241 tgcgaagcaa ttatttttca ccatggggca tgaattcgtt catgtatcac aatttgctgc
      301 attggctggt caacgtgcaa gtatattaac tcaagatttt attgatatgc tagattttca
      361 cgcgtacagc taccaaaatt caattggagg gttacaatta aacagttata caagagaaga
      421 aatattgcat tggtctacta cgtattccca gtttaactcc atgaactata ttaacttccc
      481 atggtcattt aatcattctt acatttatcc attttaacat gaaagcgata atattttata
      541 ttagtttatc catgcttttg agcatttatg ctactacata ttcccagtgt ataagtattg
      601 aactgtcagc aagatgggtc aaaggatatg atgttttcca aaaagattca ataatatgct
      661 accctgaatt aattataacc tatagaaata tgtcggatac gaattattat tttcagaaag
      721 tctcagaaag tcgcattgga ttaccaatgt tgccaattgg tcatttactg caatatccaa
      781 tagaagaata tttaaaccct gattatttaa aaagggcaaa aatgtatgga aaatattctg
      841 gtgagaacta cactgtaata tttgggggaa gttcattttc caaaggttgg attgttgtaa
      901 atgacacatc aaatattgaa gttgaacaag aaattgattt gattaatgat gatttggcaa
      961 atatttacga atacatatat cgtgagaatt atgataagca aagagattca ctaaatgagt
     1021 ataatactca ttacacttta tcggatacag taccaaaatt gatattagat aagtttaaag
     1081 acaaatttgt ttttttgaga ccgggagaga tttacacaga cacttataat cttgtcggat
     1141 ttaaattagt aaaaggatgt tttactttca atataatccc atatttgcca gcctatgttt
     1201 atactactat ttgggataaa agtcaatcaa aatacataga agtaaaaatt gaattgccga
     1261 aagtagtggg taattataaa ttatattcgg gcagttttaa ttctaataaa atagcaataa
     1321 cattttaaaa agtaaaatta acacaaaagc tggataatcc ggcattagtc ttaagatgct
     1381 gccacatttt ttgaaagaaa tccttaaact tttttttgca agcttctttt tcaattggtc
     1441 actcgctaat gtaagcattt accactgcca aaatctccag attttaatcg ttgataaaac
     1501 caaacatttc gccgagcaaa aataacatcc gctattactt tggtacttac gaaaaagaca
     1561 tcgataaatt tggaaatttc acccaaaccg attatattta caccccggca ggattaactg
     1621 caatggtgaa gaatggaaca ttgtactacg ttcataccga caatttagtc tccattcaag
     1681 ccataacaga cgaaaataaa aacatcgtca gtagctatta ttatagcttg atatgaattt
     1741 taatcctact atgtttactg cttttttcgg ttgccatatt aaagcgggtg aagcgaacga
     1801 attagtatta gcatatcaag aagttacaga agagaagata accatcaagt ccgatgtaga
     1861 gactttttca tccagtgttt ttaatggtct gtaacgatgc cactcttctg gaaataaaat
     1921 aataacacta ccaggataga ttctatatac tttgtgtttg ttttgaaaaa caccatatct
     1981 ttgtgtaata taaatgattt gatattcttg caaaattcga cctttttcta aattaaacaa
     2041 atattgcata gggtgtccca tttgaatggg gaattctgaa ttggatggaa tttcgaagtc
     2101 ccaacaactg ttaagtataa acttaatttt tggtcttgtt ttgaaaaagt agtgtattta
     2161 aatgattgta ttttcattgg gattaatatt ttcttttact ctcaattttc ttcaagataa
     2221 attgataatg taaagtaaat tattatatag attctaattt gtaaatacac gaattttttt
     2281 gaaagggata aaacctttcg aaatattata tgcagatctt gattaaaaag aataaccatt
     2341 tgtgcaatca atttttaaac tttatataaa aacaacaaaa aaatcattgt acacaataac
     2401 ataccaagaa ttattgcaca cttttttgga tattcttttt gaataaaaga acaaaatttc
     2461 tttaaaattt attgcctgct taatacttca agagcttttc tgacagtttt atcattttta
     2521 tatagcaaag gataaaaacc ttcatctcct agaatgttcc ttgaaatgta agcttttaat
     2581 tgtgttaaga taatatcttt tgagatatta atttctttct tattggcagg aacacccttc
     2641 ataagagcaa aatgaataaa attttctaaa agtggctgac tatccaaata tttttccatt
     2701 tgttgccaat ttttgaaggc tgataatttt ccgcgattct gatctgtata taaaaaggca
     2761 taccggtaaa gatatccata attaatcact ttattcagat aaggcgtata gccagtagta
     2821 tccaatggca cgtaaacatc aggcatgata ccaccacccc cgtaaacaat tctaccacca
     2881 acagttttgt atctcaaact gtctattttt tgagtactgt ctgaataatt aatatctgga
     2941 tgaataaggc gatttaaaaa atctttctca tattggtcaa cctttcctaa ttcataaggt
     3001 ttctgtatac aacgcccaga aggagtataa tatcttgcca ctgttaaacg aacagcagaa
     3061 ccatccgaaa gagtaaattg ttgctgtaca agtcctttac caaaagaacg tcttcctaca
     3121 attgttcctc tatcattatc ttgaatggca ccagcaaaaa tttcactggc tgaagctgaa
     3181 aattcattga ttaatactac taatggctca tgaatacaac tacctttacc atccgagaat
     3241 gcttcaaaac gtttataagc tctcccttgt gcataaacga ttaatctgtt ggcaggcaga
     3301 aattcattaa ccatttgaat agtttgatcc atgtaaccgc cactattttc acgtaagtcc
     3361 acaataaatt ttttagctcc ttgtcgcttt aaactggcaa tagtatttaa aaattccgta
     3421 tatgtagttt cagcaaattt acttacccga ataaaaccaa tttgaggctg aatcatgtaa
     3481 ccaatatcta ccgaatttac aggtatctca cctcttatta tctcataata aaggatttct
     3541 ggtgttccat aacgacgaac gccgactttt acttttgtac cagcgggacc acgtagtttc
     3601 ttcataactt tttcattggt gatattcttt ccaacaaaaa gtgtatcgtt cacttctact
     3661 attctgtcgc ctgccaataa acctgctttt tcagaaggtc cgccgctaat aacagaaata
     3721 atcataatcg tatcattctg aatattaaat tgtacaccaa ttccactgaa atgcccttcg
     3781 agctcactat taacatctga aacatcttcg gcagaaatat atacggaatg tggatccagt
     3841 tttgaaacaa tgtcaaccat cgttgcttct gttaactgat gcatgtccag tgtatccaca
     3901 tattttgaat taataatgga aaacaattca tcaatttttg aatgtggttt tattgcagag
     3961 aacaaagtat caactgataa attagaagct cttcttgcta aaatatttcc taaaagcaaa
     4021 ccacaaatta ttgctacaaa aactataaaa ggcgctaata atttattttt gttcatatat
     4081 agttacttta ttatttttat taaattgtta gttatcaaaa atttaaagat atttattaag
     4141 aatttctgag aaaattatac gtcaataaac actacctcaa ttcctgcccg tttaagtagt
     4201 tcaatgccat cggcaattcg atattgttct ccataaacta ctcgtttgat gcctgcctgg
     4261 ataatcaatt ttgcacattc catacagggt gaggcagtta cataaagtgt tgctccttcg
     4321 ctactgttat gggaacgggc aacttttgta atagcatttg cttcggcatg gagaacatac
     4381 ggttttgaat tgttttcttc gtcttcacaa tcattttcaa aacccgatgg agttccatta
     4441 taaccatcag aaataatcat tttatctttt actaatagag ctcccacctg tcttcttttg
     4501 caatatgaat tttcccccca gatacgagcc attcgcatat agcgttgatc cagcaatcgt
     4561 tgtttttcag tcataatctt ttctacatct tatttttaaa tgaagtaaat cattgatttt
     4621 taatcagctc atttgttaat tcttctaaat ttataccaga aaaatttcct gaagacataa
     4681 acagaacgac tgtattctca taattcaatt catgcaattt ttccagcaaa tcttctttcc
     4741 ggttaaaagc ttctactttt tctccgaaag catttttcac atcagttgga gaaaattcct
     4801 gtaactgttt ttgcttgata acttctggat cataatatac cagtgcaata tctgcttcac
     4861 tcatacttcc atagtactga ggtaaaaaat cttcggttag actgctgaaa gtatgcaact
     4921 ccatacaagc aattaacttt ttatcaggat attgctgtct tacagcttta atggtagctt
     4981 tcagtttaga aggtgagtga gcaaaatcct tgaaaacgac agtattattt ttttctgcta
     5041 ttttttgtaa acgattggat gccccagtaa agtcagaaat tattgtatag aaatcctcgt
     5101 catttactcc tatttgatgg caagcaattc gggcagcagt taaattctgc aaattatgtt
     5161 cgccaaaaat ttttaaagga acttcacgtt ttttggttat taaataagtg ataccatctc
     5221 taacttcata aggtggggta ttatagggaa atgttactat atcaggtctt aagtgctgaa
     5281 ctattttatt cagattgtca tccccttcaa aataaattaa tcttccttga aattcaataa
     5341 gatcagtaaa ttttttgaat tgatttacat attcttcaaa agttggaaac acattgacat
     5401 gatcccacgc aatacctgta agaatagcta tatgaggttg atataaatga aatttaggtc
     5461 gtggatccag tggagatgta gaatattcgt caccttcgaa aacagcaatg cgggattcgt
     5521 aggataattt taccatatta tcaaagcctt caatttgagc tcctaccata taatcggcat
     5581 taatcttcaa tttttgaaga acatataaaa tcatagctgt agtagtagtt ttaccatgac
     5641 ttccgcctac tacaattcgt gttttgctac gtgtttgata atacaaatat tcggggaaag
     5701 aatatatttt taatcccaat tcctttgctc gtaataattc aggattatcg gctttggcat
     5761 gcatcccaac gataacagca cttaatcctt tgtgaattcg ctcaggaaac catcctaatt
     5821 gttcaggcaa taatccatgt tctttcagat gagatagaga tggatcaaat atttcatcgt
     5881 ctgaaccagt aacaaaataa tcaggctttt tactcaaagc aatagctaaa ttgtgcatcg
     5941 ctgcaccacc aatggctata aaatgaactc gttgcatgct gatgaatcaa taattcaaac
     6001 tattttttaa ctttgtagat tattatcaac ttacaaaagt agtaagaaat tacgaaatga
     6061 aaaatcgcaa tggttatgaa aatctacaaa atagaagcag gtacttttca ttgtgacgga
     6121 ggagctattt ttggcgtagt ccctaaaaaa gtatggcaaa aacgttatcc ttgtaatgaa
     6181 gaaaatttct gtactctttc gatgcgttgt ttactgattg atactggtaa taaactgatt
     6241 atcattgaca gcggcacagg taataagcaa ttagagtatt tgaaatatta tgattttaag
     6301 ggagttgtca attttgaaaa agaattgaat aaattaggtt acagttgtaa tgaagtaaca
     6361 gatgtcattc ttacccatct gcactttgac cattgcggta gttgtactta ctataatcaa
     6421 aatcgaacgg aaataatgat gaccttcccc aatgctacgc actgggttgg aaagacgcaa
     6481 tgggaaaatt ttttacatcc caatgtccgt gaaggtgatt cttattttct ggaaaatatg
     6541 cagccagtat atgaagctgg caagctacaa ttggttgaga ataattttca attttgtcct
     6601 gaaattgaaa ttagaatttt taatggacat acagttggtc aattagccag ctatattcag
     6661 tataacgata aaactttaat ttatgtaggc gatgtgattc ctgttgctcc tgccattcct
     6721 ctggcatggg tatcagcttt tgatacatat cctgtgactt cgatggaaga aaaacaactt
     6781 ttattggaag aagctgtaga aaaagaacaa atcttatttt ttgagcacga tgcttatacg
     6841 gaatgttgca cagtgaaaag aaataatgat aattatcgaa tggacaaaag tttcccttta
     6901 agcaatatac aataaaaatt tcttttttga attttccagt taaaggaatt gatcaatttt
     6961 cattttgtct ttttgaatcg ttgtcttctt gataccaact ggcgtaaaaa gaataattgt
     7021 gagcaatctt acgattcatt tcattagttt gtgaaggatc aacttttttt acaaatttag
     7081 caggaacacc agcccacatc gtataaggag gaacttttgt gttactcaat attaaggcac
     7141 ctgctgcaat aatagcacct tcaccaattt cagcatcatc cagcaccacc gctcccattc
     7201 caatcagtgc ataatcatga atttttgcac catgaatagt tacattatgt cctatagaaa
     7261 catagttccc aatttcaatg gttgatttct gatataatgt gtgaagtact gaaccatcct
     7321 gcacattgac atgatttcca agccttatgg aattaacatc accacgaagc actgcattga
     7381 accagatact gcagccatca cccattatga cgtcaccaat aattaccgac gttggtgcaa
     7441 gaaaacaatt ttttccaatt tttggagtaa aacctcttac agattgaata tgtgccatag
     7501 tttatagaat tttttgcaaa ggtacaggat aattttttta atataaataa ttgttgattt
     7561 tcaaacattt gatttctctg tgaaatttat ccttgattta ttaccattaa aagtagatcg
     7621 accttccaca caaagataat gaatggaatt taatttcagt tcaacatttc gaagaaaata
     7681 ttaatttagt ctcaataaag ttattctcta tctcatttaa caagaacaaa agcaatttat
     7741 aatcactaat ttgattaagc tcaactactt ttttcacact ttttttgtaa ttttgcaaaa
     7801 aatgaaagaa acgtaaaaat gagtactgaa ctgagcgacc aagaacaata cagacgtcaa
     7861 agtttgattg aattaagaaa attaggcatc gaaccttatc cggcagcttt gtatccaaca
     7921 aatgcttttt ccgttgatat aaaaaattca ttcaaagata gcgaagaaaa gaaacaggtt
     7981 tgtgtagcag ggcggatcat gagccgtcga atcatgggga aagcttcctt tatcgaattg
     8041 caagatgcaa aaggaagaat tcaggtttat attacccgtg atgatatcag cacagaggaa
     8101 caacccgaaa tgtacaacac tgtttttaaa aagctactgg acattggtga ttttattgga
     8161 ataaaaggct ttgtttttcg gacgcaaatg ggcgaaataa ctattcatgc tcaagagttg
     8221 actgtccttt caaaatcact acgaccgtta cccattgtaa aatacaaaga tggtgtggct
     8281 tatgatgctt ttaatgatcc cgagcagcgt tatcgtcagc gatacgtaga tttggttgta
     8341 aacgagagtg taaaagatat ttttttgaaa cgcacacaaa tttttcaatc tatgcgtgat
     8401 tattttaatg cacaaggtta tgttgaagta gaaacaccta ttttacaacc tattccaggt
     8461 ggagctgctg ctcgtccatt tgtaacctat cacaatgcac tcgatatgca actttatctc
     8521 agaatagcgg atgaattata tctcaagcga ttaatagttg gcggttttga aggtgtgtat
     8581 gaattttcaa aaaatttcag aaacgaagga atggatcgaa cacataatcc ggaattcaca
     8641 gcaatggaaa tttacgtagc ctataaagat tatttctgga tgatggaatt tactgaacga
     8701 ttacttgaga aaatttgttt agatgtaaat aacagtactg aaattcactt ggaaaataaa
     8761 gttattgatt ttaaggctcc ttataaacgc atcaaaatgc tggatgtaat taaggaaaat
     8821 acaggaatcg agattgatgg aatggatgaa aatcaacttc gggaagtttg ccgtcagtta
     8881 gatattgaag tggacgaaac aatgggcaaa ggaaaattaa ttgataaaat ttttagtgaa
     8941 aaatgtgaag ctaatctggt gcaacccact tttattatag attatccaat agaaatgagc
     9001 ccattatgta aacgtcatcg caataatcct gaattaactg aacgttttga actcattgtc
     9061 aatggtcaag aactagccaa tgcttattct gagttgaatg acccgattga ccaattggaa
     9121 agattcaagg aacaactcag gttgagtgaa aaaggcgacg aagaagcgat gtttattgac
     9181 atggatttta tacgagcctt agaatacggg atgccaccaa cttcgggctt ggggattggg
     9241 atggacaggt tagtaatgct tttgactggt caacaagcta ttcaagaagt tttactcttc
     9301 cctcaaatga aacctgaaaa agtaataaaa tcggatgata aagagaaatt cttgcattta
     9361 ggaatcaaag aaccgtggct ggacgtcatg gaaaaaattg gtataaaaac aatagaacaa
     9421 ttaaaagttt ttaatcccaa taaattacat caggatctgt gcagttggaa caagaaaaat
     9481 aaacttgaac ttgttaatcc gacgattgaa gaagtaaata attggatatc aaaagcaaaa
     9541 gacaattaaa aaattaattt ctcatttttt aaaaagtttc gctgataaaa attatgtttg
     9601 aacttatttt tgaactttct tcacatgttg acaccaccat tttttatgga ataaacaatg
     9661 ttaacattca attaatcaag aatttatatc ctaatttaaa aattatagca cgaggacaca
     9721 tcataaagat catgggaagt gaagaagaag cagataagtt gtttttaaac cttcaaaaac
     9781 tggaacaata cagtattgaa aacaataaat taacagagaa aaatattatt gacctgataa
     9841 aagataatgt ccctactgat atagtaaaat atgaaaatct tattcttcac gggagtggtg
     9901 gcaaatctat tatagctcga actaaaaatc agcaaaaact ggtaaaagaa tttgaaacca
     9961 atgacttgct ttttgcaata ggaccggcgg gttcaggaaa aacttacacg gctattgcgt
    10021 tagcggttcg ttctttaaaa aataaagaag ttaagcgaat tattttaagt cgtccagccg
    10081 tagaagccgg tgaaaaatta ggttttttgc cgggtgacat gaaagaaaaa attgatcctt
    10141 acctgcaacc actttatgat gccttgcaag acatgattac acctcttaaa ttgaaagagt
    10201 atctggaaga tggcaccatt caaattgcac cgctggcatt tatgcgtggt agaactttaa
    10261 atgatgcagt agttattctg gatgaagccc aaaattctac catcatgcaa atcaaaatgt
    10321 ttcttacccg aatgggtatt aataccaaaa tgattgtaac aggtgatatc acacaaattg
    10381 atttgccacg cgatcaaaaa tcaggcttaa ttgatgcttt gcaactatta aaaaatattc
    10441 ctggaattgc tcgcgttgac tttgatgtac acgatattgt aaggcacaaa ttagttcagg
    10501 aaatcgttca ggcttacgaa aaaagaaaca atttgatgca aacaaagtcg aaaaaagaag
    10561 ataatgaaac agagaaataa atttcaatgt ttttgcaatt aaaaaattaa aaatatgaac
    10621 acactgattc gtaccaattt tcattttgaa gggcaaacaa atgtttatta tggcaaagtg
    10681 agagatgttt acaccataaa aaatgatttg ctaataatgg ttgccaccga ccgaatttct
    10741 gcctttgatg ttattcttcc acgtggaatt ccttataaag gacagatttt aaatcagatt
    10801 gctgcaaaat ttctggacga aacggctgac atcgttccga actggaaatt agcttgtcct
    10861 gatccgatgg ttacagttgg acttcgttgc gaaggttatc ctgtagaaat gattgtgcga
    10921 ggttatctat gtggcagcgc ctggagagcc tataaaaatg gagcacgtga attgtgtggc
    10981 gttaaaatcc ctgatggtat gcgggaaaat caacgttttg aaacgccaat tattactcca
    11041 acgaccaaag cagaacatgg tcttcatgac gaagacattt caaaagaaga aattctacaa
    11101 aaaggtttga tgacacctga tgaatatgag ttaattgaaa aatatgcact cgcacttttt
    11161 caacgtggca cggaaatcgc tgctcaacgt ggtttgatac ttgttgatac aaaatatgaa
    11221 tttgggaaac gagacggcaa aatttatctt attgatgaaa ttcatacgcc cgactcttcc
    11281 agatattttt atttagaagg ttatcaagaa cgttttgaaa aaggtgaacc acaaaagcaa
    11341 ctctcaaaag agtttgttcg ggaatggtta atggaaaatg gctttcaagg taaagctgga
    11401 caaaccatcc ctgaaatgac agacgaaatt gtaaaaagca tcagcgaccg ttatattgaa
    11461 ctgtatgaac aaattacagg tgaacctttt gtaagaacag acactgaaaa tctggaacaa
    11521 cgaattgaaa agaatatact tgattatcta aaaaatcatt aagctgcaaa aattaagaga
    11581 gtttaggggt ttcgaggtac gagttcgagt ttggagtttt gagcgaatga agaaaaatct
    11641 gtatttcttc atattataaa aagttcattt tcatagggtc ccacaaaaaa gatacggaat
    11701 tacacggaga aaaaattcca ttgttcgacg caccctccag gctacgtcgc aaaaaaataa
    11761 aatctcctga ttttagccgc tgataaaaat caaacgtttt gctaaacaaa tatctgtcat
    11821 aaaaacctta tcgaaaacct ttaatcttca taacagggaa tgtttcctgt agcttattaa
    11881 accttattac cttattttgt tcagttttta ataaggtaat aagg
//
LOCUS       DAOSAS010000045        11372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3810_length_11372_cov_14.686578, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000045 DAOSAS010000000
VERSION     DAOSAS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11372
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3810_length_11372_cov_14.686578"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="PLR72_03305"
     CDS             complement(<1..1336)
                     /locus_tag="PLR72_03305"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOK36268.1"
                     /translation="MKENLIAVVLLFPLSLSLYAEEEQKNDSLRTIHLNEINIYSHKE
                     TMPQQMPVSYTIITPNVINGAQFNTIRDLSIYVPNFFIPDYGSAMSTAPYIRGVGSRS
                     TGQTMALYVDNVPYFEKTTFDFDFYDIMQIDVLRGPQGTLYGRNAMGGVVNIYTLSPL
                     IYQGTKLSVAGGNYGSVMAQAAHYTKFNSKMGLSLNGYYNHRNGFFTNIYTGKDADDK
                     TSAGGRAKFNWQINSQWKTQYVFNYDYVDQTAFPYGLYDKTTGKVSNPNFNDPGAYNR
                     SMITNSLFIEYQNDKILFSSSTSHQYFSDDMKMDQDFTPLSFFSLEQIQKQNAVNEEL
                     TLKSNSKNNYQWSFGLTSFYQKLDMNAPVAFKKDAITQILQPTFDRAAAQGAPQMTIT
                     SEQMDIPGIYNTETYGGALFHQSTYNNLFIKGFSITAGLRGDFEKVKLDYNTN"
     gene            complement(1842..1914)
                     /locus_tag="PLR72_03310"
     tRNA            complement(1842..1914)
                     /locus_tag="PLR72_03310"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1879..1881),aa:Thr,seq:cgt)
     gene            complement(2007..2693)
                     /locus_tag="PLR72_03315"
     CDS             complement(2007..2693)
                     /locus_tag="PLR72_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOK36269.1"
                     /translation="MNLLETLNTLDTNLFLFLNGLHNSFFDAFMFTFSNKFIWVPLYL
                     AIAWAVIKYWKRDAVAVIFIMILCVMLTDQISSGLIKNLVQRPRPTHEESLDGLVHIV
                     NGYRGGKYGFVSSHAANSLGIAVLTAYFFRNKFYTWCILLWAVINAYSRIYLGVHYPL
                     DILGGFIVGFIVAYFCYWLIKKILPSVFQSKFDKNYHRQKVNVKIPLIILLVSIGGIF
                     IYSLVFVNIL"
     gene            complement(2870..3622)
                     /locus_tag="PLR72_03320"
     CDS             complement(2870..3622)
                     /locus_tag="PLR72_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK36270.1"
                     /translation="MKRFFLIYFFSLIFFLPLVAQSDAIKRANDFYAKGNYEAAAQEY
                     EKILKEEGFSPELYYNLGNAYYKMNEIGKAILNYERALRLSPRFEDARFNLQMAQQKL
                     VDNISESSTFFIQRWLTSLINFCTSNQWFIISFILFVVTLICAFIFFFKREVGVRKIA
                     LSCGIVFLILTIVCLTFSGIRKNEEVNHNQAIILSGVVTVKSAPDQSGTDLFQLHEGT
                     KVTIKSSLGNWVEIKIANGSVGWLETKDMEVI"
     gene            complement(3647..5461)
                     /locus_tag="PLR72_03325"
     CDS             complement(3647..5461)
                     /locus_tag="PLR72_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOK36271.1"
                     /translation="MKRKILLCLVFLTGILSAKADEPVSFTASAPSTVILDKPFQLVY
                     TINAVGKDLRIPEIKDFDILAGPFESQSSSIQIVNGKRTTSVSNSYTYTLLPKRTGTF
                     TIPSASIMVDNQKYTSNGLTIKVLPPNKESSKSEKQESSTSPSNISEENIFIRTSVSK
                     STVYEQEPVLLTYKLYTLLDVVQCINKKMPDFDGFLKQEIEQAQNKQFSYENYNGRNY
                     GTVVLYQALLYPQRPGVISIDNANFEAIIQVRNQTSIRSIFDDFFDSYTNVSKNLIAP
                     GVKINVKPLPQNKPSLFAGAVGTFTMSSSLSANQIKENEAVTLKINIAGSGNLKLIQA
                     PEIKFPNDFEVYDPKITNNFKTTLAGVQGTKTIEYLFIPRHHGEYIIPSAEFSYFDLR
                     DNVYKTLRTPEYTLHVLKGSESSTTTVVSNYSNKQEIKQLGKDIRYIYTGNIKLSRTS
                     QPLFGTLTGWLMYLITLVIAIVLFIIFFKKMKENENIVLVKNKRASKIAQKRLKTALK
                     FLKIGKKDEFYEEILKGIWNYLSDKLNIPIASLTRETIISELNKKEIDQSLIEQLIDI
                     LNSCEFARYAPQSGQQEMGDLYSDAIKIIEQLEDCIKQ"
     gene            complement(5534..6187)
                     /locus_tag="PLR72_03330"
     CDS             complement(5534..6187)
                     /locus_tag="PLR72_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK36272.1"
                     /translation="MRNKKSILILLMFIFTASTFAQKENNDIRAGNKKYNNKKYIEAE
                     VDYRKGLLKNPNSFEANYDLGNTLFRQKKYDEASKQFEKSAALSLHDKVKKAAAYHNL
                     GNSLLLQNKIEESIEAYKNALRNNPKDNETRYNLAYAQLLLKNQKKNQDKNQNQQQQQ
                     NKDKNKEDQQNKNSQPPQMTKENAQQILDALMQDEKDALKKVKKQPIKESKSSDKDW"
     gene            complement(6234..7283)
                     /locus_tag="PLR72_03335"
     CDS             complement(6234..7283)
                     /locus_tag="PLR72_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOK36273.1"
                     /translation="MLRFAHPSYFLLLLIIPVLIGIFLYSQIQKHRRLKTFGNPEVLS
                     PLMPNVSQRRPVLKFILQLIAIVFLVFALAEPQFGTRVENVKRQGIEVMIALDVSNSM
                     MAQDVAPNRLDKAKQILSKLIDQMTNDKIGLIVFAGDAYVQLPITVDYVSAKIFLSSI
                     SPKLVPRQGTAIGSAIDLAIRSFGQKSQAGRAIIVITDGENHEDNAVEAAKLAYENGI
                     MVNVIGVGTPEGAPIPIPGTMSFKKDKEGNVVVSKLNEEMCKSIAAAGNGIYVRADNT
                     NNAYRVISKQLDNLAKTTVETKIFSNYSEQFQSFVLIALLLIIIDFFIFDRKNKKLSR
                     INIFETFTPQKRKNE"
     gene            complement(7334..8317)
                     /locus_tag="PLR72_03340"
     CDS             complement(7334..8317)
                     /locus_tag="PLR72_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOK36274.1"
                     /translation="MTFHNPGYLFLLLLLIPIIFWYVWEMHKSDASLQFSSLKEIKHF
                     PKSKRIKLRHLPFILRILVIVLLIFALARPQSSNSLRNKTIEGIDIMIALDISGTMLA
                     EDLKPNRLEASKAVATEFILSRPNDNIGLVVFAAQSFTQCPLTTDHDALINLIKGVNY
                     GMIEDGTAIGLGLANAVNRIKEGKAKSKIIILLTDGSNNRGDIAPITAAEIAKTFGIR
                     VYTIGVGSHGMVKVPVQTPFGLQYQMMESEFDEQTLQEIATVTGGKYFRATDNTKLRS
                     IYQEIDRMEKTKINISEYSKKNEMYPYFVMIAFILLSLEIVLRNTILNKLP"
     gene            complement(8366..>8751)
                     /locus_tag="PLR72_03345"
     CDS             complement(8366..>8751)
                     /locus_tag="PLR72_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36275.1"
                     /translation="PPHIIALNLLDNIRNEKLWQKGKYKEYYSELTEVLRTYIERRFQ
                     IKGLEMTSSEILDSLKTLQKEHKTAYEALYQILQLADLVKFAKWIPTDEESLLSLNNA
                     YTFVNETKIEEEKTLIDDQEKEKQE"
     assembly_gap    8752..8851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8852..9081)
                     /locus_tag="PLR72_03350"
     CDS             complement(<8852..9081)
                     /locus_tag="PLR72_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36276.1"
                     /translation="MRKFLLTGIISSLIAINLNAQKITVDARLDSTVLWLGDQVHLIF
                     EVTQGKNQKVMVPLFSDTIVNGLEIVEPVKRDT"
     gene            complement(9108..9986)
                     /locus_tag="PLR72_03355"
     CDS             complement(9108..9986)
                     /locus_tag="PLR72_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOK36277.1"
                     /translation="METSEIIKKVRRIEIKSRGLSQNIFAGAYHSVFKGRGMTFSEVR
                     EYQYGDDIRNIDWNVTARFAHPYVKIFEEERELTVMLLIDVSGSSEFGSISRMKKDII
                     TEIAATLAFSTIQNHDKIGVIFFSDKIEKFIPPKKGRKHVLYIIRELVDFEPESNKTN
                     IAAALRYFTNAIKKRSTAFLISDFIAEDFENELTIANRKHDVIALQIYDIRETEIPNV
                     GLIKLKDAETGDRVWLDTSDKYLRTFYKHKWEENQLKLERIFTKAKMDAVSISTSDDY
                     VKALLKLFRMRAKIAS"
     gene            complement(10043..11038)
                     /locus_tag="PLR72_03360"
     CDS             complement(10043..11038)
                     /locus_tag="PLR72_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOK36278.1"
                     /translation="MEQPVDIRELNARIERESSFVDALIMGMNKVIVGQKHLIESLLI
                     GLLADGHILVEGVPGLAKTLAIKTLADLIKGDFNRIQFTPDLLPADVIGTMIYSQKKE
                     EFSIRKGPIFANLILADEINRAPAKVQSALLEAMQERQVTIGETTFKLEEPFLVMATQ
                     NPIEQEGTYPLPEAQVDRFMLKVVINYPKKEEEKLIIRENLEKNLPSVNPILSPKDII
                     EARDIVRQVYIDEKIEHYIIDIVFATRYPQEYGLDLLKEMIAFGASPRASINLALASR
                     AYAFIKRRGYVIPEDVRSVCFDVLRHRIGLSYEAEANNMTSEEIITEILNTIEVP"
BASE COUNT         3721 a         1927 c         1719 g         3905 t
ORIGIN      
        1 tattggtatt gtaatctaac tttacttttt caaaatcacc acgcaaacca gccgttatag
       61 aaaatccttt gataaacaaa ttattataag tggattgatg aaacaaagct cctccatacg
      121 tttccgtatt ataaattcca ggtatatcca tttgttcact ggtaatagtc atttgtggtg
      181 caccttgtgc agcagctctg tcaaaagttg gttgaagaat ttgagttatg gcatcttttt
      241 taaaagcaac aggtgcattc atatctaatt tctgataaaa agaagtgaga ccaaaactcc
      301 attgataatt attttttgaa ttagatttga gcgttagctc ttcatttact gcattttgct
      361 tttgaatttg ttccagtgaa aaaaatgata aaggtgtaaa atcttgatcc attttcatat
      421 catcagaaaa atattgatga gaagtagaag aactgaataa aattttatcg ttttgatatt
      481 caataaataa actatttgtt atcatcgaac gattataagc cccgggatca ttaaaatttg
      541 gatttgatac ttttcctgta gttttatcat ataatccata agggaaagcc gtttgatcaa
      601 cgtaatcata attaaaaaca tactgagttt tccattgaga attaatttgc cagttaaatt
      661 ttgctctgcc acctgctgag gttttgtcgt cagcatcttt accagtataa atattggtaa
      721 aaaaaccatt tctatgattg taataaccat ttaatgaaag acccattttt gaattaaatt
      781 tggtataatg agctgcttga gccataactg atccataatt tcctcctgca actgataatt
      841 ttgttccttg ataaataagt ggagaaagtg tataaatatt aacaactcca cccatagcat
      901 ttcgtccata aagcgtacct tgtggaccac ggagcacatc aatttgcata atgtcgtaaa
      961 aatcaaaatc aaaagtagtc ttttcaaaat aagggacatt gtccacataa agagccattg
     1021 tttgtcctgt actgcgtgaa cccacgccac gaatataagg agcggttgac atggctgatc
     1081 cataatcggg aataaagaaa ttgggtacat atatgcttaa gtctcttatt gtattaaatt
     1141 gagctccatt tattacattg ggagtaatga tggtataaga aactggcatt tgctgtggca
     1201 ttgtttcttt gtgagaatat atatttattt cgttgagatg gatggtacgc aaagaatcgt
     1261 ttttctgctc ttcttcagca tataaagata gagatagagg aaataacagc acaacggcta
     1321 ttaaattttc tttcattttg tttatgttta atattgttta aaatgaattg aatctccaat
     1381 aaattaaaat tcaaaacagt gaaaataaat tccttattta ttggtaacaa atagaaaagg
     1441 ttttaaaaac cactaaaaat cagcagaaaa caggaggtgg tcggcagaaa agtttgaaag
     1501 aaaatttttc agaaatttta aaaaacacct tttggatagg aagtctgatt tcgcatttgc
     1561 aaattggaat agtgaggttt ttattattct caatattaca aggaacatca gaatgaaaat
     1621 taacaacaaa agttgagttg tttaaagttt gattttcaga agaataatag ttgatgattt
     1681 ttgatgattc agaggttggg aaagttgttg aatttgtagt tgcttttggt gccagtaagc
     1741 agaacacata tcctgccaac attaaaattt ggggaatgaa agtagcaaac aatatgttgt
     1801 cgaacatcaa cattttaaaa actttttcaa caaaaaattt gagccttttt ggggactcga
     1861 accccagacc tattcattac gaatgaattg ctctaccaac tgagctaaaa aggcaattta
     1921 taaaattgca gtgcaaactt acacaaaatt tttgaaaaga cattttgaca aacaatataa
     1981 ttgccttttt attatttact atttgattat aaaatattta caaaaacaag gctataaata
     2041 aaaatgcctc cgatgctgac aagtaaaatg atgagtggaa ttttaacgtt tactttttgt
     2101 cgatgatagt ttttgtcaaa ttttgattga aaaaccgacg gtagaatttt tttaataagc
     2161 caataacaaa aataagctac aataaagcca acaataaaac ctcctaaaat atccagcgga
     2221 taatgaactc ctaaataaat tcgcgaataa gcatttatta cagcccatag aagaatacac
     2281 cacgtataaa atttatttct gaaaaaataa gcagttaaaa cggcaatacc taacgagttg
     2341 gcagcatgcg aagaaacaaa accatattta cctcctctgt aaccattaac gatatggacc
     2401 agcccgtcaa gactttcttc gtgcgtaggt cgtggtcgtt gcactaaatt tttaataaga
     2461 cccgaagaaa tctggtctgt gagcatcaca cacaaaatca tgatgaaaat cacagctact
     2521 gcatctcgtt tccagtattt tataactgcc cacgcaatag ctaaatatag tggtacccat
     2581 ataaacttgt tgctgaaagt aaacatgaaa gcatcaaaaa acgaattgtg caaaccattt
     2641 aaaaaaagga ataaattcgt atccaacgta tttaatgtct ctaacaaatt cattttctgg
     2701 tattttaaaa aaataaaaag gtacagaaaa aataaagaaa ttccttaaaa ttgactatcc
     2761 tcgccgcaag cagcgaggta ttacgccaat tttatggctt cgccaccgga atttctaaaa
     2821 aaataaaaac aaaagctacc ccgacgcaaa catcggggaa ttcttatgat taaattactt
     2881 ccatgtcttt agtctccaac catcctacac tgccattagc aatttttatt tctacccaat
     2941 tacccagtga acttttgata gtgactttgg ttccttcgtg caattgaaac aaatctgttc
     3001 cactctgatc aggagcactt tttacagtta ccacacccga aagaataata gcttgattgt
     3061 gatttacttc ctcgtttttt cgaatgccag aaaaggtcaa gcaaacaatg gttaaaatca
     3121 aaaaaacaat tccacaggat aatgcaattt ttcggacgcc cacttctctt ttaaaaaaga
     3181 aaatgaaagc gcatatcagc gtgacaacaa acaaaataaa actaataata aaccattgat
     3241 tagaagtaca aaaattgatt aaacttgtca accaacgttg aataaaaaaa gtagaagatt
     3301 cactgatatt atctaccagt ttctgttgag ccatttgtag attgaaacgt gcatcttcga
     3361 atcgtggcga taaacgcaaa gcacgctcgt aattcaaaat ggctttccca atttcattca
     3421 ttttataata agcattcccc agattataat aaagttcagg agaaaaacct tcttctttta
     3481 aaattttttc atattcttga gctgctgctt cataattccc ttttgcatag aaatcattag
     3541 ctcgtttgat agcatcactt tgagccacaa gaggcagaaa aaaaatcaat gaaaaaaagt
     3601 aaatcaaaaa gaaacgtttc ataattttaa aagtctaatt tcttttttat tgtttaatgc
     3661 aatcttctaa ttgctcaata attttaatgg cgtcagagta taaatcaccc atctcttgtt
     3721 gtcccgattg aggtgcataa cgtgcaaatt cacaagagtt caaaatatcg attaattgtt
     3781 caataaggga ttgatctatt tctttcttat ttaattctga aataattgtt tctctggtca
     3841 gagaagcaat cggtatattt aatttatcac tcaaataatt ccagattcct tttaaaattt
     3901 cctcataaaa ttcatccttt ttacctatct ttaagaattt gagagcagtt ttgagccttt
     3961 tttgtgcaat tttgctggct ctcttatttt ttactaatac tatattttca ttttctttca
     4021 ttttcttaaa gaaaataata aacaaaacaa tggctatcac caaagtgata agatacatta
     4081 accaacctgt taacgtacca aacaacggct gagaagttct gctaagttta atgtttcccg
     4141 tataaatata ccgtatatct ttccctaatt gcttaatttc ctgtttatta ctataattgc
     4201 tgacaactgt cgtagtggaa ctttcacttc ctttcagcac atgtaaagta tattcaggtg
     4261 ttcgaagagt tttataaaca ttatctctta aatcaaaata agaaaattct gcggaaggaa
     4321 tgatgtactc tccatgatga cgaggaataa ataaatattc aattgttttt gtgccttgaa
     4381 caccggcaag agttgtttta aaattgttag taatttttgg atcatacact tcaaaatcgt
     4441 taggaaattt tatttcagga gcttgaatta actttaaatt accacttccg gcaatattaa
     4501 tttttaaagt aactgcttca ttttctttta tttgatttgc agaaagagaa gagctcatgg
     4561 taaaggttcc cactgcacca gcaaaaagtg atggtttgtt ttggggcaat ggttttacat
     4621 ttatttttac cccaggagct atcagatttt tagaaacatt agtataagag tcgaaaaaat
     4681 catcaaatat gcttcgaatg gaagtttgat ttctaacttg aattatcgct tcaaaatttg
     4741 cattatcaat ggaaatgaca cctggtcgtt gaggataaag cagagcctga tataaaacta
     4801 ctgtgccata atttcgtcca ttgtaatttt cataagaaaa ttgtttgttt tgtgcttgct
     4861 caatttcctg tttcaaaaaa ccatcaaaat caggcatttt cttattaata cattgtacca
     4921 catccagtaa agtgtatagt ttataggtta ataatactgg ttcctgttcg tacacagtag
     4981 atttagaaac ggaagtacgt ataaaaatat tttcttcaga aatgttactt ggtgaagttg
     5041 aactttcttg tttttcagat ttggatgatt ctttgttagg aggtaaaact tttatggtca
     5101 gtccattgga agtatacttc tgattatcta ccatgatgct tgcagaagga atggtaaaag
     5161 tccccgttcg tttaggcaac aaagtataag tatatgaatt acttacgctt gtagtacgtt
     5221 ttccattaac aatctggata cttgaactct gagattcaaa gggacctgcc agaatgtcga
     5281 aatcttttat ttctggaata cgtaaatctt tccctactgc atttattgta taaacaagtt
     5341 gaaatggttt atccaaaata acagtactcg gtgcagaagc cgtaaaagat actggctcat
     5401 ctgcttttgc agaaagaatt ccagttaaaa aaactaagca cagaagtatt ttgcgtttca
     5461 ttggttacag tatcttcaaa ttgtctttta aaattaataa tttgtgataa atcaataagt
     5521 tacggacata attttaccaa tctttatctg aagatttact ttcttttata ggttgtttct
     5581 ttactttttt taaagcatct ttttcgtcct gcatcaaagc atctaaaatt tgttgtgcat
     5641 tttcttttgt catttgaggc ggttgcgaat ttttgttctg ttgatcttct ttgtttttat
     5701 ctttattttg ctgttgttgc tggttctggt ttttatcctg attctttttt tgatttttca
     5761 gaagtaattg tgcataagcc aaattgtagc gcgtttcatt atctttgggg ttatttctta
     5821 aggcattttt atatgcttca atactttctt caattttgtt ttgtagcaaa agtgaattcc
     5881 ccaaattatg ataagctgca gcttttttta ctttgtcatg cagagataat gcagcagatt
     5941 tttcaaattg tttacttgcc tcatcgtatt ttttttgacg gaaaagtgta tttcccaaat
     6001 cataatttgc ctcaaatgaa tttggatttt ttagcaaacc tttccgataa tccacttcgg
     6061 cttcgatata ttttttgttg ttgtattttt tattgccagc tctaatatca ttattttcct
     6121 tttgtgcaaa ggttgaagct gtaaatataa acattaataa tatcaatatg gattttttat
     6181 tcctcattgt ttatttttca tttttttctt acaccaaatt aataaaaaaa ttattattca
     6241 tttttccttt tttgaggagt aaaagtttca aaaatattaa ttctacttaa ttttttattt
     6301 tttcgatcaa aaataaagaa atcaataatt attaacaaaa gtgctatcaa cacaaatgat
     6361 tgaaattgct cgctataatt cgaaaaaatc ttggtttcca ctgttgtttt agccagatta
     6421 tccaattgtt tagaaatgac acggtaagca ttattggtat tgtcggctcg aacataaatt
     6481 ccgttcccgg cagcggcaat acttttacac atttcttcat ttaactttga aaccactaca
     6541 ttaccctctt tatctttttt gaaactcatt gttcccggaa ttggaatagg ggctccttct
     6601 ggtgtgccta cgccaattac atttaccatg atgccatttt cataagcaag tttagcagct
     6661 tctactgcat tatcttcatg attttcgcca tcggtaataa caataattgc tctaccagcc
     6721 tgacttttct gaccaaaaga tcgtattgcc agatcaatag ctgaaccaat agccgttccc
     6781 tgacgaggaa ccagttttgg tgaaatggaa gaaagaaaaa ttttagctga aacataatct
     6841 accgtaatag gcaattgtac gtaagcatcg ccggcaaata caattaaccc aattttatca
     6901 ttggtcatct ggtcaataag tttagaaaga atttgttttg ccttatccag tcggttagga
     6961 gctacatctt gagccatcat tgaatttgaa acgtctaagg caatcataac ttctattcct
     7021 tgccgtttaa cattttcaac tcttgtacca aattgtggtt cggctaatgc aaaaactaaa
     7081 aaaacaatcg caattaattg aagtataaat tttaaaactg gacgtctctg tgaaacatta
     7141 ggcattaaag gtgacaacac ctctggatta ccaaaagttt tcaggcgacg gtgcttttga
     7201 atctgactat aaagaaatat tcctattaat acgggaatta tcagcagcaa cagaaaataa
     7261 cttggatgtg caaatcgcaa cataaaatta ttttttaatc ataaaaattt ggtaaccaaa
     7321 taaatacaaa taattaaggt aatttattta atattgtatt tcgcaataca atttctaaac
     7381 tcagcaaaat aaaagcaatc attacaaaat aaggatacat ttcatttttt ttgctgtatt
     7441 cactaatgtt tattttagtt ttttccattc tatcaatttc ctgataaatg cttcttagtt
     7501 tggtgttatc agtagctcta aaatatttcc ctcctgttac tgttgcgatt tcctgaagtg
     7561 tttgttcatc aaattcggat tccatcattt gatattgtag tccaaaaggc gtctgaacag
     7621 gaactttcac cataccatgc gaaccgacac ctatggtata aactcttatt ccaaaagttt
     7681 ttgcaatttc agcagcagtt attggggcaa tatcacctct attattggat ccatcagtaa
     7741 gtaaaataat gatttttgat ttagcttttc cttctttgat gcggtttaca gcattagcaa
     7801 gacctaaacc aatggctgtt ccatcttcaa tcataccata atttactccc ttgataagat
     7861 tgatcaaagc atcatgatca gtagtcaacg gacattgcgt aaaactctga gctgcgaata
     7921 ctaccagccc aatattatca ttaggccggg acaaaataaa ttctgtggct actgcttttg
     7981 aagcttccaa ccgattgggt ttcaaatctt ctgcgagcat tgttcctgaa atatctaaag
     8041 caatcataat atcaatacct tcgattgttt tatttctcaa actgtttgaa gattgaggtc
     8101 gagccagagc aaaaataagt aaaacaataa caagtattcg aagaataaaa ggtaaatgtc
     8161 ttaattttat tcttttactt ttagggaaat gttttatctc tttcaaagaa gaaaattgca
     8221 aactcgcatc cgatttatgc atttcccaca cataccagaa gataatcggt attagtaaca
     8281 gtaataaaaa tagatatcct ggattatgaa aagtcattgt ttcattaata atttaaaaaa
     8341 ccgaaataag tttctaagaa acttgtcatt cttgtttttc tttttcctga tcgtcaatta
     8401 aagttttttc ttcttctatt tttgtttcat ttacaaacgt gtaagcatta ttcagactta
     8461 acaaactttc ttcgtctgta ggtatccatt ttgcaaattt taccagatca gctaattgca
     8521 atatttgata caaggcttca taagctgttt tatgctcttt ttgtaaggtt ttcaagctat
     8581 ctaaaatttc actcgaagtc atttccagcc ccttaatttg aaaacgcctt tcaatatacg
     8641 ttcgcaatac ttctgtcaat tcactgtaat attccttata tttgcctttc tgccaaagtt
     8701 tttcatttct aatattgtcc aataaattta aagcgataat atgcggaggc annnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtatccctt ttaacaggtt ctacaatttc
     8881 caatccattt acaatcgtat cagaaaataa aggaaccatt actttctgat tttttccctg
     8941 cgtcacttca aaaataagat gaacctggtc gccaagccat aaaacagtgg aatccaatcg
     9001 tgcatccaca gttatttttt gtgcgttcaa gttaatggct attaaagaac ttatgattcc
     9061 tgttagaaga aattttcgca taattttttt aaataagtaa gaaaaaatca gcttgctatt
     9121 tttgctcgca ttctgaataa ttttaacaaa gcttttacat agtcgtcaga ggtacttatt
     9181 gaaactgcat ccattttagc ttttgtaaat attctttcca attttaattg attctcttcc
     9241 catttatgtt tataaaaagt acgaagatat ttatctgagg tgtcaagcca gacacgatca
     9301 cctgtttctg catctttcaa ctttatcaat cctacattgg gtatttccgt ttctcgaatg
     9361 tcataaattt gcaaagcaat tacatcatgt ttacgattgg caatggtcaa ttcattttca
     9421 aaatcttcag cgataaaatc agaaatcaaa aaagccgtcg atcttttttt aatagcattg
     9481 gtaaaataac gcaaagctgc tgcaatattg gttttattac tttcaggttc aaaatcaacc
     9541 aattcccgaa taatatacaa tacatgcttt cgtccctttt taggcggaat aaatttttct
     9601 attttatcag aaaagaaaat gacccctatc ttatcatgat tttgaatcgt cgaaaaagcc
     9661 aacgtcgcag ctatttcagt aataatatcc tttttcattc tggaaatgct accaaattca
     9721 ctactacctg aaacatcaat tagtaacatt actgtcagtt cacgttcttc ttcaaaaatt
     9781 tttacataag gatgagcaaa acgagctgtt acattccaat ctatatttct gatatcatca
     9841 ccatattgat attcacgtac ttctgaaaag gtcatacctc tacctttaaa aacagagtga
     9901 tatgctccag caaatatatt ttgagataaa cctcgagatt taatctcaat tctgcgaact
     9961 ttttttataa tttcacttgt ttccatagat tttatcaata taaactaaaa acattaacac
    10021 ctgttagata aatcattgaa aatcaaggca cttctatagt atttaaaatt tctgtgataa
    10081 tttcttcaga ggtcatgtta ttagcttcgg cttcataact caagccaatt cggtgacgca
    10141 gtacatcaaa gcaaacagag cgaacatctt caggaatgac gtaaccacga cgtttgataa
    10201 aagcgtaagc tcttgaagcc agagccaagt taatagaagc acgtggtgaa gccccaaaag
    10261 caatcatttc tttcaacaga tcaagtccat attcttgtgg ataacgagta gcaaaaacaa
    10321 tatcaataat ataatgttca attttttcat caatataaac ttgccgaaca atatcacgag
    10381 cttcgataat atcttttggt gataaaatag gattgacgga aggcaggttt ttctctaagt
    10441 tttctctgat aatcaatttt tcctcttctt tttttggata gttaatcact actttcagca
    10501 taaatctatc cacttgagcc tcaggaagag gataagttcc ttcctgttcg ataggatttt
    10561 gggtagccat taccagaaaa ggttcttcca gtttgaaagt agtttcacct atggttactt
    10621 gacgttcttg catggcttct aacaaagcac tttgaacttt ggcaggagca cggttaattt
    10681 catctgccag aatcaaatta gcaaaaatag gacctttccg aatgctaaat tcttctttct
    10741 tctgactgta aatcattgtc ccaatcacat cggcgggcaa caaatcgggt gtaaactgga
    10801 tacgattaaa atctcctttt attaaatctg ctaaagtctt aattgccaac gttttagcaa
    10861 gtccaggaac gccttctacc aatatatgcc catctgccaa caaaccaatt agcaaagatt
    10921 caataaggtg tttttgacca acaattacct tgttcattcc catgatcaag gcatccacaa
    10981 aagaactttc tcgttcaatg cgtgcgttaa gttcacgaat atcaacaggt tgttccatat
    11041 tatttaattt gatttgataa aatatttttg tggaacaaat atactgtaat ttgaaacgtt
    11101 aaaatcttga tttaataact attaacctga atttagataa ttaaaacata tctttctcct
    11161 ttataagaaa ggttttggag taacttaaat atatttaaag aaagttttta aatggtgttt
    11221 ttttattggg tttgaagaca tccttactcc tatgtttttt tgaattttaa aggaaatatt
    11281 tgcagaaata agtggaaaat actacttttg caaaggaatt ggagagatgg cagagtggtc
    11341 gattgcggcg gtcttgaaaa ccgttgaact ga
//
LOCUS       DAOSAS010000046        11269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3845_length_11269_cov_12.614678, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000046 DAOSAS010000000
VERSION     DAOSAS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3845_length_11269_cov_12.614678"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLR72_03365"
     CDS             <1..243
                     /locus_tag="PLR72_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOK36279.1"
                     /translation="EGRTPLHTFRADIDYACAEALTKVGMIGVKVWICRGEIYGKKDL
                     TPSYAIGSKEIVKGDHRPGDRHSNNHSRGSKRKKNK"
     gene            271..693
                     /gene="rplP"
                     /locus_tag="PLR72_03370"
     CDS             271..693
                     /gene="rplP"
                     /locus_tag="PLR72_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOK36280.1"
                     /translation="MLQPKKTKYRRQQKGRMKGNAQRGNQLAFGSFGIKSLESKWLTG
                     QQIEAARVAVTRHMERQGQVWIRVFPDKPITKKPAEVRMGKGKGNPEGFVAPVTPGRI
                     LIEIDGVPLDVAKEALRLGAQKLPVTTKFVIRRDYEAI"
     gene            709..903
                     /gene="rpmC"
                     /locus_tag="PLR72_03375"
     CDS             709..903
                     /gene="rpmC"
                     /locus_tag="PLR72_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOK36281.1"
                     /translation="MKSREIRELSNKELVDRMELEKERLIRLKLNHAISPLDNPMEIK
                     KTRRTIARIATELRQREINQ"
     gene            918..1175
                     /gene="rpsQ"
                     /locus_tag="PLR72_03380"
     CDS             918..1175
                     /gene="rpsQ"
                     /locus_tag="PLR72_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOK36282.1"
                     /translation="MEERNLRKERIGVVTSNKMDKTITVTVKWKEKHPIYGKFINKKK
                     KFYAQDDENKCNIGDTVRIMETRPLSKLKRWRLVEIIERAK"
     gene            1178..1543
                     /gene="rplN"
                     /locus_tag="PLR72_03385"
     CDS             1178..1543
                     /gene="rplN"
                     /locus_tag="PLR72_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOK36283.1"
                     /translation="MIQQETRLTVADNTGAKEALCIRVLGGSKKRYATVGDTIVVTVK
                     SVIPSSDIKKGMVSKAVVVRTKKEIRRPDGSYIRFDDNACVLLNNAGELRGSRIFGPV
                     ARELRATNMKIISLAPEVL"
     gene            1566..1877
                     /gene="rplX"
                     /locus_tag="PLR72_03390"
     CDS             1566..1877
                     /gene="rplX"
                     /locus_tag="PLR72_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOK36284.1"
                     /translation="MSKLHIKKGDTVYVNTGVDKGRTGRVLKVLVKEQRAIVEGINLV
                     SKHMKPNARYPQGGIVKQEAPIHISNLNLVENGKPVRIGRKLNKDGELVRISKKTGEE
                     I"
     gene            1880..2425
                     /gene="rplE"
                     /locus_tag="PLR72_03395"
     CDS             1880..2425
                     /gene="rplE"
                     /locus_tag="PLR72_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOK36285.1"
                     /translation="MNTANLKQDYKERIVPNLMKEFGYTSVMQVPRLEKIVLNQGLGI
                     AVSDKKIIDTAINELTAITGQKAVATLSKKDISNFKVRKKMPIGVRVTLRGERMYEFL
                     ERLIRVALPRIRDFKGISNKLDGHGNYTLGITEQIIFPEVNIDAINRIMGMNITFVTT
                     ARSDEEGFALLKEFGLPFKKN"
     gene            2441..2710
                     /gene="rpsN"
                     /locus_tag="PLR72_03400"
     CDS             2441..2710
                     /gene="rpsN"
                     /locus_tag="PLR72_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOK36286.1"
                     /translation="MAKESLKAREVKRAKLVAKYAAKRQLLLAEGNYEALQLIPKNAS
                     PVRLHNRCKITGRPKGYMRQFGLSRIQFRELASKGLIPGVKKASW"
     gene            2846..3241
                     /gene="rpsH"
                     /locus_tag="PLR72_03405"
     CDS             2846..3241
                     /gene="rpsH"
                     /locus_tag="PLR72_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOK36287.1"
                     /translation="MTDPIADYLTRLRNAIQANHRVVEIPASNLKKEITRILFENGYI
                     LNYKFLDDGPQGTIKVALKYHPVYKVNAIKKLIRVSTPGLRKYVGYKDMPRVLNGLGI
                     AILSTSKGVMTNKEAIKQKVGGEVLCYVY"
     gene            3262..3816
                     /gene="rplF"
                     /locus_tag="PLR72_03410"
     CDS             3262..3816
                     /gene="rplF"
                     /locus_tag="PLR72_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOK36288.1"
                     /translation="MSRIGKLPIKIPTGVSVSVDNNNLVTVKGPKGTLTQKVNPIITV
                     TVKDGMCSVTRPNDEKVSRQLHGLYRALINNMVIGVSEGFKKTMELVGVGYRVSSQGQ
                     TLEFSLGYSHNIFLQLPDEIKVETKSERNQNPLIILESCDKQLLGQVCAKIRSFRKPE
                     PYKGKGIKFAGEVLRRKAGKSAGK"
     gene            3840..4184
                     /gene="rplR"
                     /locus_tag="PLR72_03415"
     CDS             3840..4184
                     /gene="rplR"
                     /locus_tag="PLR72_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOK36289.1"
                     /translation="MINKIERRQRIKLRIRNKVSGTNKKPRMSVFRSNAQIYVQLIDD
                     ENGKTLAAASSLGLKEKMTKTEQAAKVGALIAQKAKEAGITEVVFDRNGYLYHGRVKQ
                     LAEAAREGGLKF"
     gene            4190..4708
                     /gene="rpsE"
                     /locus_tag="PLR72_03420"
     CDS             4190..4708
                     /gene="rpsE"
                     /locus_tag="PLR72_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOK36290.1"
                     /translation="MTRIMNRVKTTTDLELKDRLVAVNRVTKVTKGGRTFSFSAIVVV
                     GNEDGIVGWGLGKASEVTAAIAKGTEAAKKNLIRVPIINGTIPHEQEAKFGGAKVFIK
                     PASPGTGVTAGGAMRAVLESVGITDVLAKSKGSSNPHNLVKATIAALSELRDAYTISK
                     HRGVALEKVFNG"
     gene            4723..4899
                     /gene="rpmD"
                     /locus_tag="PLR72_03425"
     CDS             4723..4899
                     /gene="rpmD"
                     /locus_tag="PLR72_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOK36291.1"
                     /translation="MATIKIKLVKSRIKSHKNQKLILQSLGLKKLNDTVEHEATPQIL
                     GMVDKVKHLVKIIE"
     gene            4943..5401
                     /gene="rplO"
                     /locus_tag="PLR72_03430"
     CDS             4943..5401
                     /gene="rplO"
                     /locus_tag="PLR72_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOK36292.1"
                     /translation="MNLNSLKPAKGSAKGRKRVGRGAGSGLGGTSTRGHKGAKSRSGN
                     VNRIGFEGGQMPIQRRLPKFGFKNINTIKYKAVNLETLEALAQNRQLTKIGLEELREA
                     GFISKNDLVKILGKGTLTVKIDVEAHAFSKSAENAISTIGGTPIKLNKDE"
     gene            5394..6737
                     /gene="secY"
                     /locus_tag="PLR72_03435"
     CDS             5394..6737
                     /gene="secY"
                     /locus_tag="PLR72_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOK36293.1"
                     /translation="MNKFIETLKNIWKIEDLRSRLLTTLLFVLIYRFGSFIVLPGIDP
                     ASLTALKAQTSGGLMALLDMFSGGAFANASIFALGIMPYISASIVIQLLTIAVPYFQK
                     MQREGESGRRKMNQITRYLTVAILLLQGPSYLVNLNMQLRQTGVALPEGFWFQASSTI
                     ILCAGSMFVMWLGERITDKGIGNGISFIILVGIIARFPNAIVAEFASRVTDESGGLIM
                     FLTEIIFLLIVIGASIMLVQGTRKIPVQYAKRIIGNKQYGGVRQYIPLKINAAGVMPI
                     IFAQAIMFIPITIVGFSKSESMRGFIGAFMDNNSFWYNFVFVILIILFTYFYTAITIN
                     PTQMAEELKRNNGFIPGVKPGKHTAEYLDIIMSRITLPGSIFLAIIAIMPAFASIFGV
                     NRSFAQFFGGTSLLILVGVILDTLQQIESHLLMRHYDGLLKTGRVKGRTGGVSAY"
     gene            6752..7537
                     /gene="map"
                     /locus_tag="PLR72_03440"
     CDS             6752..7537
                     /gene="map"
                     /locus_tag="PLR72_03440"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOK36294.1"
                     /translation="MIFLKSEEEIELLRISNQLVAKTLAELAKHIVPGISTLELNNIA
                     DEFIRDHGAIPNFLNFNGYPYSICTSVNEQIVHGMPSSKVILKEGDIISIDCGAYLNG
                     FHGDSAYTFCVGEVDPEIIKLLRTTKESLYKGIEQAQEGNRIGDISFAIQSYCEERGY
                     SVVREMVGHGIGRDMHESPEVPNYGRKGNGIMLKTGMTICIEPMINMGTRHIVIEQDG
                     WTTRTADRKPSAHFEHAVVIHHGKTEILSSFKYIEEVLADKAI"
     gene            7548..7766
                     /gene="infA"
                     /locus_tag="PLR72_03445"
     CDS             7548..7766
                     /gene="infA"
                     /locus_tag="PLR72_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOK36295.1"
                     /translation="MAKQTAIEQDGTIIESLSNAMFRVELENGHIITAHISGKMRMHY
                     IKILPGDKVKVEISPYDLTKGRIIFRYK"
     gene            7778..7894
                     /gene="ykgO"
                     /locus_tag="PLR72_03450"
     CDS             7778..7894
                     /gene="ykgO"
                     /locus_tag="PLR72_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOK36296.1"
                     /translation="MKVRVSIKKRSEDCKIVRRKGRLYVINKKNPRFKQRQK"
     gene            7911..8291
                     /gene="rpsM"
                     /locus_tag="PLR72_03455"
     CDS             7911..8291
                     /gene="rpsM"
                     /locus_tag="PLR72_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOK36297.1"
                     /translation="MAIRIVGVDIPQNKRGEIALTYIYGIGRSSANKILKEAGVDRDI
                     KAKDWTDEQIAKIREIISSNYKVEGDLRGEVQMNIKRLMDIGCYRGVRHRIGLPVRGQ
                     STKNNARTRKGRKKTVANKKKATK"
     gene            8345..8734
                     /gene="rpsK"
                     /locus_tag="PLR72_03460"
     CDS             8345..8734
                     /gene="rpsK"
                     /locus_tag="PLR72_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOK36298.1"
                     /translation="MAKKPVSAKKRVVKIDSVGQLHIHSSFNNIIVTITNEEGQVISW
                     SSAGKMGFRGSKKNTPYAAQVAAQDCAKVAYDLGVRKAKAFVKGPGNGRESAIRTVHS
                     SGIEITEIIDVTPLPHNGCRPPKRRRV"
     gene            8812..9417
                     /gene="rpsD"
                     /locus_tag="PLR72_03465"
     CDS             8812..9417
                     /gene="rpsD"
                     /locus_tag="PLR72_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOK36299.1"
                     /translation="MARYTGPKSKIARKFGEAIFGADKVLTRKNYPPGQHGNMRRRKT
                     TEYGIQLREKQKAKYTYGLLEKQFRLTYEKALRNPGITGIILLQLLESRLDNIVYRFG
                     MAPTRAAARQLVSHKHIVVDGKVVNVPSYQVKPGQIVGVREKDKAMEVILNSLNGFNH
                     SKYPWLEWDANTLSGKYLYIPEREDIPENIKEQLIVELYSK"
     gene            9433..10425
                     /locus_tag="PLR72_03470"
     CDS             9433..10425
                     /locus_tag="PLR72_03470"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOK36300.1"
                     /translation="MAILAFQKPENVIMLEYGDFHGKFEFRPLEPGYGITIGNALRRV
                     LLSSLEGFAITSIKIEGVDHEYSTIPGVIDDVTNIILNLKQVRFKQIVEGVDNEKVTI
                     KVSNTTAFKAGDIGKYLTGFEVLNPDLLICELDESANFQINITINKGRGYVTAEENRL
                     PNVEFGVIPIDSIFTPIRNVKYQIENYRVEQVTNYEKLVLEVDTDGSIHPKEALKEAA
                     MILIQHFMLFSDEKISVEIEEREESEEFDEEILRMRQLLKTKLVDLELSVRALNCLKA
                     ADIETLGQLCSYQKHDLLKLRNFGKKSLSELEEKLESLNLTFGMDVSKYKLDKE"
     gene            10431..10955
                     /gene="rplQ"
                     /locus_tag="PLR72_03475"
     CDS             10431..10955
                     /gene="rplQ"
                     /locus_tag="PLR72_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOK36301.1"
                     /translation="MRHNKKFNHLSRKAAHRKSMLANMACSLIFHKRIATTVPKAKAL
                     RVYIEPLLTKAKSDSTHSRRVIFSYLQNKEAVTELFQNIAVKIADRPGGYTRILRTGY
                     RVGDNAEMCIIELVDYNENMLKEKVTKKTSRTRRSSKKKTSEDTASATSLTQNTPLIE
                     NKEQTIDSEIPENN"
BASE COUNT         4157 a         1523 c         2207 g         3382 t
ORIGIN      
        1 gaaggcagaa ctcctcttca tacttttaga gctgatatag attatgcttg tgctgaagct
       61 ttaacaaagg tagggatgat aggagtgaaa gtatggattt gcagaggtga aatttatggt
      121 aaaaaggact tgactccttc ttatgctatt ggaagtaagg aaattgtaaa aggtgaccat
      181 cgcccaggtg atcgtcattc aaataatcat agcagaggtt caaaaagaaa aaaaaataaa
      241 taatcttata agaatttgat ttaaaccatc atgttacaac cgaagaaaac taaatacaga
      301 agacagcaaa aaggacgcat gaaaggaaac gctcagcggg gcaatcaatt agcttttggt
      361 agttttggaa tcaaatcatt ggaatctaaa tggttgacag gccagcaaat tgaagccgct
      421 cgtgttgctg taacacgaca tatggaacgt caaggacagg tttggattcg tgttttcccg
      481 gataaaccca ttacaaaaaa accagcagaa gttcgtatgg gaaaaggtaa aggaaatccc
      541 gaaggatttg ttgctcctgt tactccagga agaatactta tagaaattga tggtgtacct
      601 ttagatgttg ctaaagaagc tcttcgttta ggtgcacaga aattgcctgt aacaacaaag
      661 tttgtcatac ggcgtgatta tgaagcaatt taaaaatgca tagcaaaaat gaaatcaaga
      721 gaaattagag agttatcaaa taaagaactc gtcgatcgaa tggagttaga aaaagaacgt
      781 ttaatacgct taaagttgaa tcatgctatt agtcctctcg ataatccaat ggagattaaa
      841 aaaacacgta gaactatcgc acgtattgca actgaattac gtcagcggga aattaatcaa
      901 taaattttta ttatctgatg gaagagagaa atttaagaaa agaacgcata ggagttgtta
      961 ccagtaataa aatggataaa actattacag taaccgtaaa atggaaagaa aaacatccta
     1021 tttacggtaa atttataaat aaaaagaaaa aattctatgc tcaggacgat gaaaacaaat
     1081 gtaatattgg tgatacagtg cgtattatgg aaactcgtcc tttgagtaaa ttaaaaagat
     1141 ggcgattagt agaaattatt gaaagagcta aatagttatg atacagcaag aaacaagact
     1201 aacagtagct gataatacag gagcaaaaga ggctctttgt attcgtgtat taggcggaag
     1261 taaaaaaaga tatgccactg tgggtgacac aatagttgta acggttaaaa gtgtaatacc
     1321 ttcttcagat attaaaaagg gaatggtgtc aaaggcagtg gtagtacgta ctaagaaaga
     1381 aattcgccgt cccgatggtt cttacattcg ttttgatgat aatgcttgtg tattactcaa
     1441 taatgcagga gaacttagag gaagtcgtat ttttggacct gtagctcgtg aattacgtgc
     1501 tactaatatg aaaatcatat ctcttgcacc agaagtgctt taattctttg aaaaaatata
     1561 aagtgatgag caaattacat ataaagaaag gtgatacagt ttatgtcaat actggtgttg
     1621 ataaaggcag aactggaaga gttttaaaag tgttagtaaa agaacaacga gctattgttg
     1681 aaggcattaa cttagtttcc aaacacatga aacctaatgc cagatatcct caaggaggaa
     1741 tagtaaagca ggaagctccc attcatattt ccaatttgaa tttagtggaa aatggtaaac
     1801 cggttcgcat tggaagaaaa ttaaataagg atggagaatt agtaagaatt tctaaaaaaa
     1861 ctggagagga aatttaagaa tgaatacagc aaacttaaaa caagattata aagaacggat
     1921 agttcctaat ttaatgaaag aatttgggta tacttctgtt atgcaagttc cacgattaga
     1981 aaaaattgta ttaaatcaag gtttaggaat tgccgtatca gataaaaaaa ttattgatac
     2041 tgctataaat gaattgacag ctatcacggg tcaaaaagca gttgctacct tatcaaagaa
     2101 agatatttca aatttcaagg taagaaaaaa gatgccaatt ggcgtcagag ttactcttag
     2161 aggcgagaga atgtatgaat ttttggaacg tttaattcgt gttgcattac cgcgtattcg
     2221 tgattttaag ggaataagca acaaattaga tggacatgga aattatacgt taggtataac
     2281 agaacaaatt atttttcctg aagtgaatat agatgctatc aatcgtatta tggggatgaa
     2341 cattactttt gttacaacag cacgcagtga tgaagaaggt tttgctttat taaaagaatt
     2401 tggattaccg tttaagaaaa attaataaaa agaaagaaac atggccaaag aatcattaaa
     2461 agcacgggaa gtgaaaagag caaaattagt tgccaaatat gcagctaaac gccagttgtt
     2521 acttgctgaa ggaaattacg aagcccttca attaattccg aagaatgctt ctcctgttcg
     2581 tttacataat cgttgtaaaa taacaggacg tcctaaaggg tatatgcgtc agtttggctt
     2641 atcccgtatt caatttcgtg aactggcttc gaaaggatta atccctggtg taaaaaaagc
     2701 aagctggtaa ttaaaaaaaa ttgataattt tcctaataaa gagttggctt taaaaaagtt
     2761 tagcgagaag aggaaaataa aattctaaga aacagaaaaa ttaaatatta tcccggcagt
     2821 cgggaccaat ttaaaaaata aatatatgac agatcctatt gcagattatt taactcgttt
     2881 gcggaatgca atacaagcaa atcatcgtgt ggttgaaatt cctgcatcaa atttaaaaaa
     2941 agaaataact cgtattttat ttgaaaacgg ttatattttg aattataagt tcttagacga
     3001 tggacctcaa ggtactatca aagtagcttt aaaatatcat ccggtttata aagtaaatgc
     3061 tatcaaaaaa ttaattcgtg tttctacacc tggacttcgt aagtatgttg gctataaaga
     3121 tatgccacgt gtgctgaatg gattaggaat agctattctt tctacttcaa aaggagtgat
     3181 gactaataag gaagcgatta agcaaaaagt aggaggagaa gttttatgtt atgtttatta
     3241 aagtaaagag gagagaaaat tatgtcgaga attggtaaat tacctataaa aattcctaca
     3301 ggagtttctg taagtgtaga taacaataat ttagtaacag ttaaaggacc aaaaggaaca
     3361 cttacacaaa aagttaatcc aattattaca gtgactgtta aagatggaat gtgcagtgta
     3421 actcgtccaa acgatgaaaa agtaagtcga caattgcatg gattgtatag agctctgatt
     3481 aacaatatgg taattggagt ttcagaagga tttaaaaaaa ctatggaatt ggtaggggta
     3541 ggttacagag tttcttcaca agggcaaaca cttgaattct ctttgggata ttcgcataac
     3601 atttttttgc agcttcctga cgaaataaaa gtagaaacaa agagtgaacg taaccaaaat
     3661 cctcttatta ttttagaatc ttgcgacaag caattattag gtcaggtttg tgctaaaatt
     3721 cgttcgttca gaaaaccaga accttataag ggaaaaggaa ttaaattcgc aggtgaagtt
     3781 ttacgtcgaa aagctggaaa atcagcgggt aaataatcta atacgtaaat ttagaaatta
     3841 tgattaacaa aatagaaaga agacaacgca taaaattacg tattcgtaat aaggtttcag
     3901 gaacaaataa aaaaccaaga atgagtgttt ttagaagcaa tgctcagatt tacgttcaat
     3961 tgatagacga tgaaaatgga aaaactctgg cagctgcttc ttctttaggt ttgaaggaaa
     4021 aaatgactaa aactgaacag gcagcaaaag tgggtgcact cattgctcaa aaagctaaag
     4081 aagctggaat tacagaagtt gtttttgacc gtaatggtta tttatatcat ggaagagtta
     4141 aacaattagc agaagcagca cgtgaaggcg ggcttaaatt ttaagattta tgacaagaat
     4201 aatgaataga gttaaaacga caactgatct tgaattgaaa gatagattag tagctgttaa
     4261 tcgtgtaacg aaagttacaa aaggaggaag gacttttagt ttctcagcta ttgttgtagt
     4321 tgggaacgaa gatggcattg ttgggtgggg gcttggaaaa gcaagtgaag taactgctgc
     4381 tattgcaaaa ggtactgaag ctgcgaagaa aaatttgatt agagtgccta ttatcaatgg
     4441 aacaattcct catgaacagg aagcaaaatt tggcggagcc aaagtattta tcaaaccagc
     4501 ttctccaggt actggcgtta ctgcaggggg tgcaatgaga gctgtattag aaagtgtagg
     4561 gatcacagat gtattggcaa aatcaaaagg ttcttcaaat ccacataatc tggtaaaagc
     4621 caccattgct gcattaagtg agttacggga tgcttatacc atttctaaac atcggggagt
     4681 agctcttgaa aaagtgttta atggatgata aaatacagaa aaatggcaac tataaaaatc
     4741 aaactggtta aaagtagaat aaaatcacat aagaatcaaa aattaatttt acagtcccta
     4801 ggactaaaaa aattaaatga taccgttgaa cacgaggcta cacctcaaat attaggaatg
     4861 gtagacaaag taaaacattt ggttaagatt attgaataaa ctcagaattt ttagaataat
     4921 ttatttaata cgataaaata ttatgaattt aaacagctta aaaccagcta aaggctcagc
     4981 aaaaggaaga aaaagagttg gacgtggagc aggttcaggt ttaggtggta cttcaacaag
     5041 aggacataaa ggtgcaaaat cacggtctgg taatgttaat agaattggtt ttgaaggtgg
     5101 acaaatgcct attcaacggc gtttgccaaa atttggtttt aaaaatatta ataccattaa
     5161 atataaagct gtcaatttag aaacattgga agctcttgct caaaatcgtc aattaacaaa
     5221 aattggtctt gaagaattac gagaagctgg ttttatatca aaaaatgatt tagtaaaaat
     5281 tttagggaaa gggacattga cagtaaaaat agatgtagaa gctcatgctt tttcaaaatc
     5341 tgcggaaaat gctatcagca ctatcggtgg aactcctata aaattaaata aggatgaata
     5401 aattcattga aacccttaaa aatatttgga aaatagaaga cttaagaagt cgcttattaa
     5461 caacattatt atttgtatta atttatcgtt ttggttcttt tattgttctt ccaggtattg
     5521 atcctgcttc cttaactgct ttaaaagctc agacaagtgg ggggttgatg gctttattgg
     5581 atatgttttc tggaggtgcc tttgctaatg cttctatttt tgctttgggt atcatgccct
     5641 atatttctgc ttctattgta attcaattgt tgacaatagc tgttccttat tttcaaaaaa
     5701 tgcagcgtga aggggaaagc gggcgtcgga aaatgaacca gattactcgt tatcttactg
     5761 ttgctatttt acttttacaa ggtccttctt atttagtcaa tttgaatatg cagttaagac
     5821 agacaggtgt tgcacttcct gaaggattct ggtttcaggc ttcttcaacc attattttat
     5881 gtgctggcag tatgtttgta atgtggttag gagaaagaat taccgataaa ggtatcggaa
     5941 atggaatttc atttatcatt ttagtaggaa ttattgcgag gtttccaaat gctattgttg
     6001 cagaatttgc ttcacgagtt actgatgaaa gcggaggctt aatcatgttt ctaacagaga
     6061 ttatattttt gttaattgtt attggagctt ctattatgct ggtacaaggg acacgaaaaa
     6121 ttccagtaca atacgcaaaa cgtataattg gcaataaaca atacggaggt gtaagacaat
     6181 atattccttt aaaaattaat gcggcaggag taatgcctat tatatttgca caagctatta
     6241 tgtttattcc tattactatc gtaggttttt caaaatctga atctatgaga ggttttattg
     6301 gagcatttat ggataataat agcttttggt ataattttgt ttttgtaatt ttgattattt
     6361 tatttactta tttttatacc gccatcacga ttaatccaac ccaaatggct gaggagttaa
     6421 aacgtaataa tggttttatt ccgggagtta aacctggtaa acatacagca gaatatttag
     6481 atataattat gtctcgaata acattgcctg gctcaatatt cttggcaata attgcaatta
     6541 tgcctgcttt tgcaagtatt tttggtgtga atcgttcgtt tgctcaattt tttggaggaa
     6601 cttccttgct gattttggtt ggagttattt tagacacctt gcaacagata gaaagtcatt
     6661 tattgatgag acattatgat ggcttattga agactggaag agtaaaaggt cgtactggag
     6721 gggtctctgc ttattaaatt tattatttga gatgattttt ttaaaatctg aagaagaaat
     6781 cgagttactt cgcataagca atcaattggt ggctaaaacg ttagccgaac ttgcaaaaca
     6841 tattgttccg ggtataagta ctttagaatt aaacaatatc gctgatgaat ttatcaggga
     6901 tcatggggca ataccaaatt ttcttaattt taatggttac ccttattcta tttgtacttc
     6961 agtaaatgag caaattgttc atggaatgcc ttcttcaaaa gtaattttaa aagaaggaga
     7021 tattatctcg attgattgcg gagcttattt gaatggtttt catggcgatt cggcttatac
     7081 tttttgcgtg ggagaagtgg atcctgaaat aataaaatta cttcgtacta cgaaggaatc
     7141 attatataaa ggaatcgaac aggctcaaga agggaatcga ataggtgata ttagttttgc
     7201 aattcaaagt tattgtgaag aacgcggtta ttcggtagtt cgcgaaatgg ttggacatgg
     7261 aattggacgc gatatgcatg aatctcctga agttcctaat tatggacgaa aaggaaatgg
     7321 aattatgctg aaaactggta tgacaatatg tatagaaccc atgattaata tgggaacccg
     7381 tcatatagtt atcgaacaag atggatggac tactcgtaca gcagatcgaa aaccatcagc
     7441 tcattttgag catgctgtag taatccatca cgggaaaaca gaaattttat cgagttttaa
     7501 atatatagaa gaagtattag ctgataaagc aatttaaagg ttaatttatg gccaaacaaa
     7561 cagcaataga acaagacgga acgataatcg aatcattgtc gaatgccatg tttcgcgttg
     7621 aattagaaaa tggtcatatc attaccgctc atatttctgg aaagatgcgt atgcattata
     7681 ttaaaatttt accaggcgac aaagtaaaag ttgaaatatc tccttatgac ttaacaaaag
     7741 gaagaattat ttttagatat aaataaatag aataacaatg aaagttagag tttcaataaa
     7801 aaagcgttca gaagattgta aaattgttcg tcgaaaaggg cgactttatg tgattaataa
     7861 aaagaatccg agattcaagc aacgtcaaaa ataatttaaa agattaacat atggctataa
     7921 gaatagtagg tgtagatata ccacaaaaca aaagaggaga aattgctttg acttatattt
     7981 atggaatagg tcggagtagt gctaacaaaa ttttaaaaga agctggcgta gatagagaca
     8041 ttaaagccaa agattggaca gatgaacaaa ttgctaaaat acgcgagata attagttcaa
     8101 attataaagt agaaggagat ttgaggggag aagtgcaaat gaatatcaaa cgtttgatgg
     8161 atataggttg ttatcgagga gttcgtcatc gtattggttt accagtaaga ggtcaaagta
     8221 caaagaataa tgcacgaacc cgaaaaggaa gaaagaaaac tgttgccaat aaaaagaaag
     8281 caactaaata ataattaatt taaaaaaata ataatttaac tgagaaagaa aaaattaaat
     8341 taatatggca aaaaaaccag tttcagccaa aaaaagagtt gtaaaaatag atagtgtagg
     8401 tcaattacat atccattctt cattcaataa tattattgta acaattacga atgaagaagg
     8461 tcaggtaatt tcatggtcat cagcaggtaa aatgggtttt aggggttcta aaaaaaatac
     8521 tccttacgct gcccaagttg cagctcagga ttgtgcaaaa gtagcctatg atttaggagt
     8581 tagaaaagct aaagcttttg taaaaggtcc aggcaatggt cgtgaatcgg ctatccgcac
     8641 agtacatagt tctggaattg aaataaccga aattattgat gtaactccac ttcctcataa
     8701 tggttgccgt ccacccaaaa gaagaagagt ttaaatattt aatatacaat aaattacaga
     8761 aaatagtgat tatttgttaa gatttaattt tatattttta aagcataaaa aatggcaaga
     8821 tatactggac caaaatcaaa aatcgcacgt aaatttggtg aagcaatttt cggtgctgat
     8881 aaagttttaa cacgaaaaaa ttatcctcct ggtcaacatg gtaatatgcg aaggcggaaa
     8941 actacggaat atggtattca attacgagag aaacagaaag caaaatatac ttatggttta
     9001 ttagaaaaac aattccgtct tacctatgaa aaggctttac ggaacccagg tatcacagga
     9061 attattcttt tgcaattgtt ggaatcccgt ctggataata ttgtttatcg tttcggaatg
     9121 gctcctacac gagcagccgc ccgtcaactt gtaagtcata aacacattgt tgtggatggc
     9181 aaagttgtaa atgtcccttc ttatcaagtt aaaccgggtc aaattgttgg ggtacgcgaa
     9241 aaagataaag ctatggaagt aattctaaat tcattgaatg gatttaatca tagtaaatat
     9301 ccttggctgg aatgggatgc caatacttta tcaggtaaat atttgtatat tcctgagaga
     9361 gaagatattc cagaaaatat taaagaacaa ttaattgttg agttatactc taaataataa
     9421 aaaatttaat tcatggctat attagcattt caaaaacccg aaaatgttat catgttagaa
     9481 tatggtgatt ttcatggaaa atttgaattt cgccctttag aacctggtta tggcattact
     9541 attggtaatg ctttgcgacg agttctattg tcctctttgg aaggtttcgc catcacttca
     9601 atcaagattg aaggcgtaga tcatgaatat tctactatac cgggtgtcat tgatgatgtg
     9661 accaacatca ttcttaactt aaaacaagtt cgttttaagc aaattgttga aggtgtcgat
     9721 aacgaaaagg taactatcaa ggtttcaaat actactgcct ttaaagctgg agatattgga
     9781 aaatatctaa ctggctttga agtgcttaat ccagatcttc ttatttgtga acttgatgaa
     9841 agtgcgaatt ttcaaatcaa tattacaatt aataaaggac gtggttatgt gactgctgaa
     9901 gaaaacagat tacccaatgt tgaatttgga gtaattccta ttgattcaat ttttactcct
     9961 ataagaaatg taaaatatca aatagaaaat tacagggttg aacaagtaac aaattatgaa
    10021 aaattagttc tcgaagtgga tactgatggt tcaattcatc caaaagaagc attaaaagaa
    10081 gcagcaatga tattaattca gcattttatg cttttctctg atgaaaaaat ttctgtagaa
    10141 attgaggaaa gagaagaaag tgaagaattt gatgaagaaa ttttgagaat gcgccaattg
    10201 ctaaaaacaa aattagtaga tcttgagtta tctgttcgtg ctctcaattg tttaaaagca
    10261 gcagatattg aaacgttggg tcaattatgt agttatcaga aacatgattt gttaaagctg
    10321 agaaattttg gcaaaaaatc acttagcgaa ttagaagaga aattagaaag tttaaatctt
    10381 acttttggga tggatgtttc taagtataaa ttagataaag agtaaaaaag atgagacata
    10441 ataaaaaatt caatcattta agtcgaaaag cggctcatcg aaaatcaatg ctggcaaata
    10501 tggcctgttc actgatattt cacaaaagaa ttgccactac tgttcctaaa gccaaagctt
    10561 tacgagtata tattgagcca cttttaacaa aagcgaaaag tgatagcact cattcacgcc
    10621 gtgtcatatt tagttattta caaaataaag aagctgttac agaattattt caaaatattg
    10681 ctgtaaaaat agctgatcgt cctggtggat atactcgtat tttacgtaca ggctatcgcg
    10741 tgggggataa tgctgaaatg tgtattatag agttagtgga ctataatgaa aatatgttaa
    10801 aagaaaaagt aacaaagaaa acttcacgga cccgtcgttc ttctaaaaag aaaacctctg
    10861 aagataccgc ttctgctaca tctttaacac aaaatactcc attaatagaa aataaagaac
    10921 aaacaataga ttcagaaatt cccgaaaata attgattttt agtatttaaa ggttaagaga
    10981 gattatattt atataagttt aaaagttaat tgccacaaag cagaagcatt ttttcatcag
    11041 tttttagaat ataaggtacc atttgtcgtt aatttatttg tttcaaatct caataaaatt
    11101 tattctattc ttaccaatta ggaaactatc attataaaac tttaaaaaaa ttaacgacaa
    11161 atggtgcact gtaaaattac aaggagaaaa acaagccggt gaaagaattg agcttattat
    11221 taagctaatt ttcaatataa atgtttttaa atttaatttt gatttttta
//
LOCUS       DAOSAS010000047        11241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3855_length_11241_cov_14.608439, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000047 DAOSAS010000000
VERSION     DAOSAS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3855_length_11241_cov_14.608439"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1176)
                     /locus_tag="PLR72_03480"
     CDS             complement(7..1176)
                     /locus_tag="PLR72_03480"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOK36302.1"
                     /translation="MKLNRENRQENLKNKLNSNAGRDGKFYRKSDREDMPAEEGNKDL
                     FLTENNRIQVGKKLILKKKENSFSKTSNFLNRNNKSKDYSSDRKNENDLILKKKKNVT
                     SFPKYEKVTYNKTSSHPLKGENDRKDFHKSPYSQKKQLEYNKKNIDYSKPIRLNKFLS
                     NAGVCSRREADEFIKAGVISVNGQVVTEMGTKVMRTDKIMFRNQEVHLERKVYILLNK
                     PKDCVTTSDDPHARLTVIDLVKNACSERIYPVGRLDRNTTGVLLLTNDGDLAAKLTHP
                     KYDKKKIYQLTLDKSLKPEDYENIKKGVALENEELIEIDALEFIKEGDRKQLGIEIHS
                     GQNHVVRRIFEKFGYKVIRLDRVYFAGLTKKNLSRGKYRFLTQKEINMLKMGAYE"
     gene            complement(1397..1837)
                     /locus_tag="PLR72_03485"
     CDS             complement(1397..1837)
                     /locus_tag="PLR72_03485"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOK36303.1"
                     /translation="MEEEKRKIDLGKKNNEIIYSKSIKAGNRIYYLDVKKSRRDELYL
                     AITESKKKKVGFDENAQVTYEKHKIFLYREDFDKFREGLEDVMNFIKNQGEEETKTES
                     NLEEDQKSNEISDDPGEDDSVSDELDKEGNNPKPEKNFFDKFKF"
     gene            2089..>2975
                     /gene="nusB"
                     /locus_tag="PLR72_03490"
     CDS             2089..>2975
                     /gene="nusB"
                     /locus_tag="PLR72_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOK36304.1"
                     /translation="MINRVLIRIKIIQILYSYYKNEGKSVQLAEKELLHSIEKTYDLY
                     YHLLQLAIEITRYAALRIDARKNKLRPTAEDLHPNMKFVENKFVQQLSNNIQFNSYLN
                     EHKLSWTNYPEIIKNLFEEITTADFYKEYMESSLDDYEEDKNLWKKIYKKIILENEEL
                     DNSLEDQNIYWTADIELVVSFIIKTIKKFKLENSAYQELLPMFKDEEDEEFARKLLRS
                     SINNCNEFRNLINKNTKNWELDRIAFMDIVIMQAALAELMEFPTIPVNVTLNEYIEIS
                     KMYSTEKSGKFINGILDNIV"
     assembly_gap    2976..3075
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3076..3303
                     /gene="yajC"
                     /locus_tag="PLR72_03495"
     CDS             <3076..3303
                     /gene="yajC"
                     /locus_tag="PLR72_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOK36305.1"
                     /translation="FFLIRPQTKRQKEIKKQRDAMKPGDHVITSGGIYGKIKEVKDNA
                     FIIEIADNVRIKVDKNSVFLTAEDIQNTPSK"
     gene            3364..4305
                     /locus_tag="PLR72_03500"
     CDS             3364..4305
                     /locus_tag="PLR72_03500"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HOK36306.1"
                     /translation="MKMFFLSKDVFIFLFFLVLSTGFWFINALSKPREKTIEVPLQYE
                     GLPPDIAITNSPPKFISLKIRDEGRNLLSYTRKKLNPLIIQLARNFQENGEIIISTDQ
                     LLNKFTYYLQPTTLILEAKPETILIRYEKLYSKVLPVECRINFDLLPQYMISDEIKLQ
                     PSKVNVFGPKHLLDSLKTIKTEPIYLKSLHDTTIITAKLQSIASVRFLPETVEVIIPV
                     EMFTEKKVQLPITVINAPGDIYLRTLPSKVIATFNIAMSRYHSFKPSDIQVAVDYNEV
                     KNNNSDKLNVKILRYPIYVQNIRLDPQQVDFFVEKNK"
     gene            4355..4825
                     /locus_tag="PLR72_03505"
     CDS             4355..4825
                     /locus_tag="PLR72_03505"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOK36307.1"
                     /translation="MLQRIQTLYLTIVLILSCLTLFSPVANLFNKEDLTFYEINYRGI
                     CLIENDSKTPVSSVWALTAISAIVPLVTLTTIFCYKKRMLQIRLSIFNLIIMIGYYVI
                     LFIYIWFATKNLPASHWNLKIVSAFPLINIILTILAMRAIVRDERLIKSLNRLR"
     gene            complement(4914..5591)
                     /locus_tag="PLR72_03510"
     CDS             complement(4914..5591)
                     /locus_tag="PLR72_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOK36308.1"
                     /translation="MVGTLVNTGAVIIGGTIGLLLNKSMPERFKTIYFQAIGLFTIAI
                     GISMVWNMKHIIIVVGSLAIGALLGEWMNIEKGAEQFSEYLKRKLHLGGERFSEGLVT
                     AFLLFCIGSMTILGAIQEGIDGSSDLLFTKSLMDGFSSIILGSALGAGVIVAAIPLLI
                     FQGGITLLAMWASSFFTSEIIQGLTSVGGILLIGLGIDILGIKKLRIINLLPALIFVV
                     LFLWWFV"
     gene            complement(5754..8135)
                     /locus_tag="PLR72_03515"
     CDS             complement(5754..8135)
                     /locus_tag="PLR72_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36309.1"
                     /translation="MNNQTSSIRINVAIGLISVAIIAFQLTLMQILSYVQWYHFAYMI
                     ISIALLGFGAAGTLLTIFREKLEQNYFYLFPLLLIVTAILIPFVVLIANSEAIRFDSL
                     LIFHDSRYVVKLIATYFIFFLPFLTGALVIGLSFSKFANQIGKIYFSNLIGSGVGGII
                     ALFLMQFVIPEQQPFFIAIIAFLGGVISIPKNAGIFLRIVTLISIIILFVLFFKPPQL
                     VPSEYKDISKTMLLPEATVEYKKPSPHGFVQIVSSPVLRYAPGVSLAYQDSFPVRKAV
                     FNNGDWAGYVVPPFPDKNKPSILNYTPQALPYHIKKIEKALILNAGTGENVSLALSQH
                     VPFITANEANAKIFNLLQHSFADFDEVKLYQTMARTVLITSDIASYDLIELPVIGSFF
                     GNSGLNAVEPRYELTIEALEEIWNKLSDDGMISLSCWMDYPVRNAYRLLSTTGLLLDK
                     NKVENCLKHVVGIRSWGAVTFLVKKSPFSEEEIRKVHRFCDEWMFDPLILSETKEFER
                     DKFNMLQDTTFFVNVDHLLSPSKKTFIQQYTYRIHPTTDNCPFFFQFVRFNAIPKLIS
                     SIGKGNIPFLDIGYLLIILTFIQIVIIAAIFILLPLLFKKWESKNKKWVFIYFSGLGL
                     AYMFLEMVFIQQFTFYFGEPTYATALTLSILLFTSGLGSYYSGMVQNNNKARYVLPII
                     LATILILYAITLSPILTSTIGLALPFKILISVILLGILGFLLGMPFPLGVNYLSEKNN
                     QDIPWGWAFNGYFSVISTVLATIISVEVGYLSLFIIAAFLYALVGISNRWIKI"
     gene            complement(8191..8892)
                     /locus_tag="PLR72_03520"
     CDS             complement(8191..8892)
                     /locus_tag="PLR72_03520"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOK36310.1"
                     /translation="MKKLSIFIIILLNSGLFFPLYGQDKYERERKKMIKNQIQSRGIT
                     DKKTLEAIESVPRHLFVPDEYRDRAYDDGPLPIGYGQTISQPYIVAYMTELLNVGPDD
                     IVLEIGTGSGYQAAVLSRIVKKVYTVEIIEELGLAAKERLKALNYDNVEVKIGDGYYG
                     WEEHAPYDGIIVTAAAEFIPPPLIEQLKDNCNMIIPVGSPFNIQNLMLVKKSGGKITT
                     KSLIPVRFVPLTRKK"
     gene            9684..10556
                     /locus_tag="PLR72_03525"
     CDS             9684..10556
                     /locus_tag="PLR72_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36311.1"
                     /translation="MNKQQDKFMTELTRLLESQNFQSEEEMEKFLNDLMGKDISSFKN
                     KPSTNKEKAQDLVYEAYDLTPAKAKKKIEKALQLDPDCIEAYEYLGDIEESIQKADAF
                     YEKGIEIGRRLFCGEKYNEYKGYFWGIVETRPFMRCLRDHAEILYLLGKKKECVEIFE
                     EMIEFNPNDNQGVRDQLMLYLIELNEDSKYEKYFKMFKNEDKAFSLFNYALYTFKKEG
                     DTLKSKKQLQKALKQNKYVAKKILSAISIVELAEYYGIGDENEADYYVSYAQPIWWET
                     KGAIDWLRKYSTMS"
     gene            10727..11164
                     /locus_tag="PLR72_03530"
     CDS             10727..11164
                     /locus_tag="PLR72_03530"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HOK36312.1"
                     /translation="MKPTEKQIKNIAESLDSGMRSFFHLKTGEIKELPNFENWLDTST
                     SDLWDDDVKEIEKNSEDYFEFEDIDSYEFYQIMTDFAESVEDIRLQERLLQALSGSKP
                     FRNFKWQIEYSGNYREKWFDFKMRKYVEWVKEQIEENKEKFNE"
BASE COUNT         4024 a         1774 c         1639 g         3704 t
ORIGIN      
        1 taaaaatcat tcataagctc ccattttgag catattgatt tctttttgtg taaggaaacg
       61 atattttcct cgtgaaagat ttttcttggt caaacctgca aaatagactc tatctaaacg
      121 tattacttta tagccaaatt tctcaaatat acgtcgaacc acatgatttt gacctgaatg
      181 aatttcaata cccaattgtt ttctatcacc ttcttttata aattcgagtg catcaatttc
      241 aatcaactct tcattttcaa gtgcaacgcc ttttttgata ttttcgtaat cttctggttt
      301 taatgattta tcgagagtta attgataaat ttttttcttg tcatatttgg gatgggttaa
      361 ttttgcagcc agatcaccat cgtttgtcaa caacaggaca cccgtggtat ttctatccaa
      421 cctgccaacc ggatatattc gttcactgca agcattttta accaaatcta tcaccgttaa
      481 acgagcatgt ggatcgtcag aggttgttac acaatctttt ggtttattaa gcaagatata
      541 gactttccgt tccagatgaa cttcttgatt acgaaacatt atcttgtcag ttctcatcac
      601 tttggtcccc atttcagtaa ctacctgacc attaaccgaa ataacacctg ctttgataaa
      661 ttcatcagcc tctctgcgtg aacatacgcc tgcattggac aaaaacttgt ttaatcgaat
      721 aggctttgaa taatcaatat ttttcttatt atattcaagt tgtttttttt gactataggg
      781 cgatttatga aaatctttcc tatcattttc accttttaaa ggatgagaac tggttttatt
      841 ataagttact ttttcatatt tcggaaaaga ggtaacattc ttcttctttt ttagtataag
      901 gtcattttca ttttttctat ccgaactata atctttgctt ttattatttc tgtttaaaaa
      961 attagaagtt ttagaaaatg aattttcttt tttctttaaa atcaatttct ttcccacctg
     1021 aatgcgatta ttttctgtta ggaacaagtc tttatttccc tcttctgctg gcatatcttc
     1081 tctgtcactt tttctataaa atttaccgtc acggccggca tttgaattta atttattttt
     1141 taaattttct tgcctatttt ccctgtttaa tttcattttg atttgataaa aaatttacat
     1201 tgcaaatttc ataaaaaaac ttggataaat cattatccta taagtcaaaa aactctaata
     1261 aattaacaat ttattttaaa aagataattc tgtcaaaatt atctcaaaag gaaagtataa
     1321 aaatacactg ctatcccttt tttgaacagc agtgtattaa gagaataaaa acagacattg
     1381 gtcgagaagt gagaatttaa aatttgaatt tatcaaaaaa atttttctcg ggttttggat
     1441 tattaccttc tttatccaac tcatcactca cagaatcatc ttcacctgga tcatcgctta
     1501 tttcattaga tttttgatct tcttcaaggt tactttctgt ctttgtttct tcttcacctt
     1561 gatttttgat gaaattcatt acatcctcta atccttcacg aaacttatca aaatcttcac
     1621 gataaagaaa tattttgtgc ttttcgtaag taacctgagc attttcgtca aaaccaactt
     1681 tctttttctt gctttcagtg atggctaaat acaattcatc tcgtctgctt ttttttacat
     1741 ctaaataata aatacgattg cctgctttga tagattttga ataaataatt tcattattct
     1801 ttttccccaa atcaattttt cttttttctt cttccatgtt ttccatcttt ttcagttaga
     1861 ttttaaataa aatagagaaa aatcccttca aaaatttaaa gttcaaattt ctatattttt
     1921 ttttacttta tcaaattatt tatctatttt ttgattttaa attatgttac ttcatttttg
     1981 ggaatagatg tgaaagtaat tttaacagac agtagtatga aaaagaagaa aaaataccta
     2041 actttgcctt ctaatttata aagtgaccat tgaagattaa ctgttgatat gattaatcgt
     2101 gttttaattc ggattaaaat tattcaaatt ttgtattctt attataagaa tgaaggtaaa
     2161 tccgtacaat tagctgaaaa agagcttctt cacagcatag aaaaaactta cgatctttac
     2221 tatcatttat tgcaacttgc tattgaaatt actcgttatg ctgctttgcg tatcgatgcg
     2281 agaaaaaaca aactgcgtcc cacagcagaa gatttgcacc ctaacatgaa atttgttgaa
     2341 aataaattcg ttcagcagtt atcaaacaat atccaattta atagttattt aaacgaacat
     2401 aaattatcgt ggacaaacta tcctgaaatc attaaaaatt tatttgaaga aatcacaaca
     2461 gcagattttt ataaggaata tatggaaagt tcgctggatg attatgaaga agataaaaat
     2521 ctatggaaga aaatttataa aaaaattatc cttgagaatg aagagctgga caactcactg
     2581 gaagatcaaa atatttattg gacggctgat attgaattgg tagtgagttt cattattaaa
     2641 acgataaaaa aattcaaatt agaaaatagt gcttaccaag aacttttgcc aatgtttaaa
     2701 gatgaagaag atgaagaatt tgctcgaaaa cttttaagaa gttctattaa taactgcaat
     2761 gaatttagaa atctaattaa caaaaacacc aaaaattggg aattggatcg aattgctttt
     2821 atggatattg ttattatgca agcagctctg gcagaattaa tggaattccc aaccattcca
     2881 gtgaatgtta ctttaaatga atacattgaa atttccaaaa tgtatagcac tgaaaaaagt
     2941 ggcaaattta ttaatggcat cctcgacaat attgtnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnttttt ccttatcaga ccacaaacaa aacgtcaaaa agaaataaaa
     3121 aagcaacgtg atgcgatgaa accgggtgac catgtaatta cttccggagg aatttatgga
     3181 aaaataaaag aagttaaaga caatgctttc attattgaaa ttgctgataa cgtgcgaatt
     3241 aaagtagata aaaactctgt atttcttact gctgaagata ttcagaatac tccgtctaaa
     3301 taaagtcgga accttatgtt ccctaataac ttaataaaaa tattaaagtt ttacggagca
     3361 aagatgaaaa tgttctttct ctcaaaggat gttttcatct ttttattttt tcttgtactt
     3421 tctacagggt tctggtttat taatgcattg agcaagccac gtgaaaagac aattgaggtt
     3481 cctctacaat atgaaggatt gccacctgat attgctatta cgaattctcc accaaaattt
     3541 atttctttaa aaatcagaga tgaaggcaga aatcttttga gttatactcg aaaaaagctg
     3601 aatcctttga taattcagtt agcaagaaat tttcaggaaa atggagaaat cattatttct
     3661 acagatcaac tcttgaataa attcacttat tacctacaac ctactacact cattttagaa
     3721 gccaaaccag aaaccatttt aattagatat gaaaaacttt atagcaaggt tttacctgta
     3781 gaatgcagaa tcaattttga tcttttgcct cagtatatga ttagcgatga aataaaatta
     3841 cagccgagta aagtaaatgt ctttggacca aaacacttat tagattctct gaaaactata
     3901 aaaaccgaac ccatttattt aaaaagcttg catgatacta ctattataac agctaaactt
     3961 caatcgatag cgagtgtaag atttttacct gaaactgtcg aagttattat tcctgtggaa
     4021 atgtttacgg agaagaaagt tcaactgccg atcactgtaa taaatgctcc tggtgatatt
     4081 tatttacgaa ccttaccatc aaaagttatt gccactttca atatcgcaat gagtcgttat
     4141 cattcgttta aaccttctga tattcaggtg gcagttgatt ataatgaagt caaaaacaat
     4201 aattctgata aattaaatgt aaagattctc agatatccta tttatgtgca aaacattcgt
     4261 ttggatcctc aacaagttga tttttttgtt gagaagaata aatgatttat ttattgattt
     4321 atttattaat tcactatctt taaaataaat tattatgtta cagcgtattc agacattgta
     4381 tttaacaatt gttcttatac tttcctgtct tacacttttt tctcccgttg ctaatttgtt
     4441 taataaagag gatttaactt tttatgaaat aaattatcgt ggtatttgtc tgattgaaaa
     4501 tgattctaaa acgcctgttt cgtcagtatg ggctttaact gccatttcag ctattgttcc
     4561 attagttaca ctgacaacta ttttctgtta taaaaagaga atgcttcaaa tacgtctttc
     4621 cattttcaat ctcattataa tgattggata ttacgtaata ttgtttattt acatctggtt
     4681 tgccaccaaa aatttacctg cttcacactg gaatttaaaa attgtttctg cttttccgct
     4741 cataaacatt atactgacaa ttttagctat gagagcaatt gtcagagatg aaagattaat
     4801 taagtcacta aatcgattac ggtaaacgta aaaaataatt tttgttggct tttataaagt
     4861 gaaaaagtga tgattttttt aatttcaggg aaaaatattt ttccttaaaa taattaaaca
     4921 aaccaccata aaaacaaaac aacaaaaatc aaagcaggca gtaaattaat gattcttagt
     4981 tttttaatgc caagtatatc aattcctaaa cctattagta aaattccacc cacgctggtt
     5041 aatccttgaa taatttccga agtaaaaaat gaggaagccc acatagccag taaagttatt
     5101 ccaccctgaa aaattaacaa tggaatagca gccacaataa ccccagcacc taatgcagaa
     5161 cctaaaataa tagaagaaaa accatccatt aatgattttg taaacaacaa gtcagaagag
     5221 ccatcaatgc cttcctgaat ggcacctaaa atagtcattg agccgatgca aaacaacaga
     5281 aaagccgtca ccaaaccttc tgaaaagcgt tcacctccca aatgaagttt tcttttcagg
     5341 tattcactga attgttctgc acctttttca atgttcatcc attctcctaa taacgcacca
     5401 atcgccaaac tacctactac aataataata tgcttcatat tccataccat actgatccca
     5461 atggcaatag taaacaaacc aatagcttga aaatatattg ttttaaaacg ttctggcatg
     5521 cttttattta acagtaaacc aattgttcca ccaataatta cagcacctgt atttactaaa
     5581 gtccctacca tgattttaaa aaattagata atttgatttg aatttcagta tgataatttt
     5641 ttacaaaaat actaaaactt caacacaaaa aatttttctt tgctttgaca aggttgctat
     5701 tttatcgatc aaaaatttac caccaattat ttaatcgcat ttattggata aaatcatatt
     5761 tttatccacc tatttgatat gcctaccaaa gcatatagaa aagctgcaat tataaatagc
     5821 gaaagataac caacctcaac agaaataata gttgccaaaa cagtactgat taccgaaaaa
     5881 taaccattaa aagcccagcc ccatggaata tcctgattgt ttttttcaga taaataattg
     5941 acacctaaag ggaaaggcat tcctaaaagg aaacctaaaa tacctaataa aattaccgaa
     6001 atcaatattt taaaaggtag tgctaaccct attgttgaag tcagaatagg agataatgta
     6061 attgcgtata aaataagtat tgtggcgaga attattggta aaacatatcg tgctttattg
     6121 ttattttgga ccatacctga gtagtagctt ccaagtccag aagtaaaaag taaaatactc
     6181 agagtcaacg ccgtagcata agtaggttcg ccaaaataaa aagtaaattg ttgaataaaa
     6241 accatttcca aaaacatgta agctaaaccc aacccactga aatatataaa aacccacttt
     6301 ttatttttac tttcccattt tttaaaaaga agaggcagaa gaataaaaat tgctgcaata
     6361 ataacaattt gtataaaagt aagaatgatt aataaatacc cgatatccaa aaaaggaata
     6421 ttacccttcc cgatagaaga aatcaacttc gggatagcat taaaacgaac aaattgaaag
     6481 aaaaaaggac aattgtcggt agttggatga attcgatagg tatactgttg aataaacgtt
     6541 tttttgctgg gcgataataa gtgatcaaca ttaacaaaaa aagttgtgtc ctgaagcata
     6601 ttaaatttgt cgcgttcaaa ctctttagtt tcagaaagta tcaatggatc aaacatccat
     6661 tcatcgcaaa aacgatgaac cttcctaatt tcttcttcac tgaatggcga tttttttact
     6721 aaaaaagtaa ctgctcccca acttctaatg ccaacaacgt gttttaaaca attttcaacc
     6781 ttatttttat cgagaagaag tccagtggtt gacaaaagtc tgtatgcatt tctaacagga
     6841 taatccatcc agcaacttaa ggaaatcatg ccgtcgtcag aaagtttgtt ccaaatttcc
     6901 tctaaggctt ctatagttaa ttcgtatctg ggctcaacag cattaagtcc tgaattgcca
     6961 aaaaatgagc caataacagg aagttctatt aaatcataac tggcaatatc ggaagtaatt
     7021 aacacagtac gtgccatagt ttgatagagt tttacttcat caaaatcagc aaaagaatgt
     7081 tgaagtaaat taaaaatttt agcattggct tcattagctg taataaaagg cacatgttgc
     7141 gataaagcca gcgagacatt ttctcctgtt ccggcattaa gaatcaacgc cttttcaatt
     7201 tttttaatat gataaggcaa cgcttgcgga gtgtaattta aaatacttgg tttgtttttg
     7261 tctgggaatg gcggaactac atatcctgcc caatcgccat tattgaaaac agctttacgt
     7321 acaggaaaag aatcttgata agcaagacta acaccaggag catatcgaag cactggtgaa
     7381 gacactatct gtacaaaacc atgcggactc ggttttttat attcgacagt agcttcgggt
     7441 aataacatag ttttactaat atctttgtat tcggaaggta caagttgagg tggcttaaaa
     7501 aataagacaa ataatataat gattgaaatc aaggtaacta ttcgaagaaa tatccctgca
     7561 tttttcggaa tcgaaataac tccacccaaa aaagcaataa tagcaatgaa aaaaggctgt
     7621 tgttcaggaa ttacaaactg cataaggaat aaagcaataa tccctcctac tcctgagcct
     7681 ataagattag agaaatatat tttcccaatt tgattagcaa atttcgagaa acttaaacct
     7741 atgactaagg caccagtcaa aaaaggcaga aaaaaaataa aataagtagc aatcagttta
     7801 acaacataac gactatcgtg aaaaatgagc aaagaatcga aacggatagc ttcggaattt
     7861 gcaatcagca ctacaaaagg tataagaata gctgtaacaa ttaacagcaa aggaaaaaga
     7921 tagaaataat tttgttcgag tttttctcgg aaaatcgtca atagcgtacc agcagctcca
     7981 aaaccaagca aagctatgga tataatcata taagcaaagt gataccactg aacgtacgac
     8041 aaaatctgca tcaatgtaag ttgaaacgca ataatagcaa ccgaaatcaa cccgattgct
     8101 acattgatgc gaattgatga cgtctgattg ttcatctcat tctttcttta ccaaaatttc
     8161 agtgatttat ttataatgtt ctgtttgact ttattttttt ctagttaaag gaacaaaccg
     8221 gacaggaatc aagctttttg tcgtgatttt acctcccgat tttttaacta acattagatt
     8281 ttgaatattg aaaggagaac ctaccggaat aatcatatta caattatctt tcaactgttc
     8341 aattaatggg ggcggaataa attcggctgc tgcagtaact ataataccat cataaggagc
     8401 atgttcttcc caaccataat aaccatcacc tattttaact tctacattgt catagttcag
     8461 tgcttttaac cgttcttttg cagcaagacc aagttcttca ataatttcga cagtatacac
     8521 ctttttcaca attcttgata gaacagcagc ctgatagcct gaacctgttc caatttctaa
     8581 cactatatca tcaggaccaa cattcaataa ttctgtcatg taagccacga tataaggttg
     8641 tgatatggtt tgcccataac ctatgggaag aggaccatca tcataagctc tatcacgata
     8701 ttcatcgggg acaaacaaat gacgtggaac actttctatt gcttctaatg tttttttatc
     8761 ggtgataccg cgtgactgga tttgattttt tatcattttt tttctctctc tttcatattt
     8821 atcctgaccg taaagaggga aaaaaagtcc actatttagt aggataatta taaaaatgga
     8881 taattttttc atattctctt tttttttcag gaacgttaat ggaaaaaata ttaatatttg
     8941 actttacttt taaaagtctc tattactaat tattcattaa aatatgtatt gacgatcaat
     9001 aacttatgaa ccaaaactat taaaatcgat ttttcagggt agattcattt atcatggttt
     9061 aatttttcat ttaaaaatta tatcaaaaca aatttaatat cttattttga taataaagaa
     9121 tttttcctta aaaaaatatt atattcctct tcgtttgtgg taagaattag gaagagctaa
     9181 aaagcattat aaaaattaca gctcaaagaa atgcaggtaa aaacaagaaa ccgaaataag
     9241 tattacctta gtggtggaga agatagtact gaaaactttt cttttgttcg aaaaaatatg
     9301 tttaatttgt cgaaaaaaac atgccaaaag atttaatgat tccaacatgc ttaaaagttg
     9361 cttggaacaa tcaattcctt ttacaattat tggaaattaa atgcgtcggt cctgaacaaa
     9421 ataaagaatt tctatgtatc tattcaaact attaagtaca tttgtgtttt taatcagata
     9481 taatttaaca agatattatt ctgaagaaat aggaaatcgt atgataatac gtttaatata
     9541 gaacaaaatg atctctttta aacaagaata aaaaagcaaa gtgaataaaa atcatcgatc
     9601 gcagatcttt tttatgggta gtaatggtta aaaaaagcat taaactttaa aatttttgtg
     9661 aattttttaa aaaagaaaaa attatgaata agcaacaaga caaatttatg actgagttaa
     9721 caagacttct tgaatctcag aattttcaga gtgaagaaga aatggaaaaa tttctgaatg
     9781 atcttatggg gaaagatatt tcgtctttca aaaacaaacc atcaactaat aaagaaaaag
     9841 ctcaagacct tgtttatgaa gcttatgatt taacgcctgc taaagcaaaa aagaaaatag
     9901 aaaaagcact tcaacttgac cctgactgta ttgaagctta tgaatatttg ggtgatatag
     9961 aagaatcgat tcaaaaagca gatgcttttt atgaaaaagg tattgaaata ggcagacgtt
    10021 tattttgcgg agaaaaatat aatgagtata aaggttattt ttggggaatt gttgaaactc
    10081 gaccttttat gcgttgttta cgagatcatg ctgaaattct ttaccttctc ggtaagaaga
    10141 aagaatgtgt cgaaattttt gaagaaatga tagaattcaa tccgaacgat aatcaaggag
    10201 taagagatca attaatgctt tatctgattg agctgaacga ggatagcaaa tatgagaaat
    10261 atttcaaaat gtttaaaaac gaagacaaag ctttctctct ttttaattat gctttatata
    10321 ctttcaaaaa agagggtgat actttaaaat ccaaaaaaca attacaaaaa gccttaaaac
    10381 aaaataaata tgtagcaaaa aaaattttat cagctatatc catcgttgaa cttgcagaat
    10441 attacggaat aggagatgaa aatgaagctg attattacgt atcttacgcc cagcccattt
    10501 ggtgggaaac caaaggtgca atagactggt taagaaagta tagtacgatg tcttaactgt
    10561 taaatatttt tattttcttt tttgtttact gttgaaattt gtattatctc attatttctg
    10621 tcattttcta tatagagata ataaatctgc acatgtgaaa taatttaatc tttaagaaaa
    10681 atactttcat gctctttgtt attcaaacat taaaaatctc ttaaacatga aacccacaga
    10741 gaaacaaata aaaaacattg ctgaaagttt ggatagtggg atgaggagtt tttttcatct
    10801 caaaacaggg gaaataaaag aattacctaa ctttgagaat tggttggata catccactag
    10861 cgacctttgg gatgacgatg ttaaagaaat tgaaaaaaat agtgaagatt attttgaatt
    10921 tgaagatatt gattcctatg aattttatca aattatgacc gattttgcgg aaagtgtgga
    10981 agatataaga ttacaggaga gattgttaca agcactaagc ggctccaaac cttttcgcaa
    11041 cttcaaatgg caaatagaat attccggcaa ctatcgagaa aaatggtttg attttaaaat
    11101 gaggaaatat gtagaatggg tgaaggaaca gattgaagag aacaaagaaa aattcaatga
    11161 ataatcattt tacttaaatt tgtaaatagg tacattgagc tatattctca acggttcgac
    11221 atacaaaaaa tatgaaagta a
//
LOCUS       DAOSAS010000048        11189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3874_length_11189_cov_14.921412, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000048 DAOSAS010000000
VERSION     DAOSAS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11189
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3874_length_11189_cov_14.921412"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..883
                     /locus_tag="PLR72_03535"
     CDS             2..883
                     /locus_tag="PLR72_03535"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOK36313.1"
                     /translation="MLYKLFLLKIEIDKKSGFCFGVVKAIAKAEEELAKGGPLYCLGD
                     IVHNEEEVKRLEKMGLITIDYEQFKQLHEVKVLIRAHGEPPLTYQIARENNISLIDAT
                     CPVVLKLQSSIKKTYEAISSEEQIVIYGQEGHAEVRGLVGQTNDNAIVVENEEDLKKI
                     DFTKKIHLFSQTTKDLESFNHLATTISEKIKKKENLKISDTICRQVANRIPNITNFAQ
                     QHDIILFISGKKSSNGKVLFEHCKKVNPNTFYIYNCDQIDDLPIDLTKKIGICGATST
                     PKWLMECVAEKLRQKVN"
     gene            919..1902
                     /gene="pfkA"
                     /locus_tag="PLR72_03540"
     CDS             919..1902
                     /gene="pfkA"
                     /locus_tag="PLR72_03540"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOK36314.1"
                     /translation="MEYSIKCIGLLTSGGDAPGMNAAIRAVTRTAIFNGIRIKAIYRG
                     YRGLITGEIKEFKTENVSNIIQQGGTILKTARCDEFMTLEGRKLAYENMQKEEIDALI
                     VIGGDGTFTGARIFAQEFNVPIIGIPGTIDNDLYGTDVTIGFDTALNTIIEAVDKIRD
                     TASSHERLFFVEVMGRDAGFLALNSALASGAEAAIIPERETKVDQLEEVIKNGFRKSK
                     NSSIVIVTESKVTGGANGLATRMRKEHPEYDVRVTILGHIQRGGSPSVSDRILASRMG
                     AAAVEALLDEQRSVMIGVVNNEIVNVPFTKAIKYNKPIDEDLLQIIQVLSI"
     gene            complement(1973..2719)
                     /locus_tag="PLR72_03545"
     CDS             complement(1973..2719)
                     /locus_tag="PLR72_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOK36315.1"
                     /translation="MKLYGLIGYPLTHSFSKKYFTEKFEKENIDARYNLYELENIDLF
                     PELKNIPELAGLNVTIPYKEQIIPYLDELDDTAKEIGAVNVIKFIRNGKILKLKGYNT
                     DVIGFEKSILPFLQPYHRKALILGTGGVSKAIYFVLQKLGIETTFVSRRPCKGMLSYE
                     MLDNQTIRENKLIINATPLGTFPHADECPAIPYPFLTNEHLLFDVVYNPPETLFLRKG
                     KLQGAKGVNGEKMLEEQAEAAWKIWNEPEK"
     gene            complement(2716..3453)
                     /locus_tag="PLR72_03550"
     CDS             complement(2716..3453)
                     /locus_tag="PLR72_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOK36316.1"
                     /translation="MKNKPIILLVFLFTFLSFNIQTTEAQKNISNLKTVQELSLLQKI
                     ERWYNKNMTYSAICALMTIESSFIPFPSEIIVPPAAYIASQPDSNLNHLLIILFATLG
                     SLMGAYINYALSISLGRALIYKLADSKVGAALLLNSEKIRKAEAYFNKYGNISTFIGR
                     LIPGIRQLISIPAGLAKMNFFKFSLYTVLGAGVWNCVLAYIGYLAHGQADLINKYNKE
                     ISIVMVILVVAAGIFGGIKYYLRLKKK"
     gene            complement(3476..4846)
                     /locus_tag="PLR72_03555"
     CDS             complement(3476..4846)
                     /locus_tag="PLR72_03555"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK36317.1"
                     /translation="MSRKIFFQNDDDINEIIHRYEQFLSGKASGYFDIEEFENIAEYY
                     LQKGRTKDSSRAIEFGIKLHPTSSELKTKRAKIYLASGNAEKAYQLLESLADKNDYEV
                     ILLKIQALVHMRRLSEAYQLCNRVIDFENEGKDDVLLDIAYIYIEHGYFNEAMEWLLK
                     GYKINPKNSDLLFELAFCYEQLDEDEKAIVIYNKIIDLDPYSSEAWFNLGQIYFTSQE
                     FSKAIEAYDFVLAIDENDSLACLQKAHAHFQLNQFKEAIESYKEYAELTSDSEQANIY
                     IAESFEKMDQFDDAISYYQRSLKIQPDNYDALLGIGACLLEKEKFKESIQFISKALHI
                     NKEDTDAWNYLGEDYLRMDDIDNALLCYLKSITLDPSQPETLMIIANINLEKGDYNTA
                     LEFYLQVLQQDSTIEYLYLFLAITYYKIGNKTLAYAYLKKAIQENEEASSMFLEICPE
                     AIDPIL"
     gene            5117..5650
                     /locus_tag="PLR72_03560"
     CDS             5117..5650
                     /locus_tag="PLR72_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOK36318.1"
                     /translation="MDFFEKFQFCPACGSPFFIKNNEKSKRCQQCDFVFYMNASAAVA
                     AFILNEKNELLVCKRKKEPAKGTWDLPGGFVDNNETAEAAVKRELAEELHAETVQVEY
                     LFSEPNEYEYSDLVVPTLDLFFLCCLKDESQLIPSDDVEDCFYVALDKLEPDYFGLKS
                     IRKAIIRFIELTLGKAE"
     gene            5725..8760
                     /locus_tag="PLR72_03565"
     CDS             5725..8760
                     /locus_tag="PLR72_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK36319.1"
                     /translation="MKKSFFLFSFLCVITIPLLNAQYTLIYTDPNASFDKGKELYFQR
                     KYTASYHYFEDFLKTAKPQQAGQMHEAEYFLAANAFELRQKNAMELLNNFLLKYPYTS
                     YADPVYAMIGMLYYEQKNYSEAIIYFEKVKENNLNPDKRIDFLFCKGYAYLENKEYKK
                     ASDIFKDLKSVESPQKISAIYYYAYSQYLLGNYEEALPEFLKIKNETDYQAIVPYYLV
                     QIYYIQKDYKLARQEAFSLLENYPDNKNNAEIYRILGEIAYNEKNYKDAVFYLTKYEK
                     MFPQVLRNDMYILGLSYFQLKDYKNAVVYLSKVTTTRDEISENAYLYLGSSYIYLNDK
                     INARLAYEAALETNFNPAVREEALFNYALTCYETPSAFGETISAFTRFLSEFPNSKYS
                     DEAYRYLAEAYLTTKNYELAYQSISQIKQLMPQLSETKQYLLYKLGSQAFVQNDYEKA
                     IDYFTRALEISSSNKYAAECLFWRAECFSRLQKSAQAQNDLTAFFKNAYAKSSVNFIM
                     ANYSMAYAYFSQKKYSEALNWFLKYINLESNSSSVTYVDALNRIGDCYYNARNFEQAL
                     KYYDKVVQLSPETADYALFQSAYIEGIKKNYTAKISKLEKLLTLYPGSEYNDNALYEI
                     ARSYLMLNNNEKAIAIYQRLINQFPNSVLARKSMLEIGMIYLNEKNYDKAITTFKNVI
                     EKYPESEEFYTALESLETIYVEINDVNAYLNYAASLGQKIKNVAEQREDSISYIAAEK
                     QYLNGKYEVALTGLSSYVNKFCPGGRHCIIARYYLADCYYQTGEKEKALSTYQALLEL
                     PVSEFTEEAALRCAEITYDKKDYSSALNYFKKLETVAQSTINKNAARLGILRCNYFLN
                     DYQSLVNIASEIIADPRSDESLLIESLYYRAKSYLELGKNEQAIVDLEKLSVDTRTTW
                     GAESKYLLANVYFEQGKLKEAENEIMDFMKKNTPHQFWLAKSIVLLADIYVKKGDDFQ
                     AKQYLLSLRKNYLNHDEIENLIEERLQAIAKREKQKIIG"
     gene            8807..10555
                     /locus_tag="PLR72_03570"
     CDS             8807..10555
                     /locus_tag="PLR72_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36320.1"
                     /translation="MNRKKLLTFIFIVAINFMSVFGQDSIFRREVTVEREYVPVIREA
                     GKINLTPKLYEPQVEKLSPVYTEFYLPLPVETNIHPLQAIEFSLQREPLKNGYTKIGL
                     GNYYNSLLDFAYPFIKKEDMYLDFIVHHLSTYGNKWHTTSDASLFFNKDFKNLDFFTG
                     VNASYERLKYYGNNFNLDSIVDLKQLAGNYPLAIYLPTDKEQPIFSYSLKELSELNRF
                     DTFWRGNIYGGVQSKSNVTDIQYVGKLDYSFLNASSGSKEHQILLSGKFQIPYNDNKL
                     GLDADLFQLNYNSKVTDSTNIENSYSVFIIHPYYDYHSDKFRARLGIQSAFSFEEKGF
                     LHLYPDIQAEWQPFPTWMAIYGGITGNHQVNSRSKILKENCFAEAYLKVNDTFTPFEG
                     YLGIKLKPAELFFVDAFIDYQHINNQYFFINKEFLSNDLREPNNHLFINRFDVTYSKT
                     DLFKSGLRLSYHLQDRFSCELKGVYNDWQVSDEQYAWNKPKWEADMNFQASLTAGVNL
                     SAKIFYQGGRYAKLGEKAISMKSAFDVNMGISYNYSRFLTVFAKVNNFFNSTYQQFYG
                     YDVQGTNFLLGAALSF"
     gene            10603..11130
                     /gene="rimM"
                     /locus_tag="PLR72_03575"
     CDS             10603..11130
                     /gene="rimM"
                     /locus_tag="PLR72_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445025.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOK36321.1"
                     /translation="MISKEEVFPIGEIIKPHGVKGEMSFLLKHPISDWEKIPFLIFSR
                     DGILVPFFIENIRWKSDNGGFLKLEDINTETAAHKFSGLTIFLHKKFLNHVISLEEEE
                     LQAEYFIGFQVINQENVLIGTIQEVDQSTENILFVVEKEGQEYLIPLSEEYIKNIDHK
                     KKEIFMELPEGLLDL"
BASE COUNT         3812 a         1636 c         1941 g         3800 t
ORIGIN      
        1 aattttatat aaactttttc tcttgaaaat agaaatagat aaaaaatcgg gtttttgttt
       61 tggagttgta aaagcaattg ccaaagctga agaagaattg gcaaaagggg gacctcttta
      121 ttgtttaggc gatattgttc acaatgaaga agaagtgaaa cgattagaaa aaatggggtt
      181 aatcactatt gattatgaac aatttaaaca gttacatgag gtaaaggttt tgattcgtgc
      241 tcatggcgag ccaccgctta cttatcaaat tgctcgagaa aataatattt ccttaattga
      301 tgcaacgtgt cctgtcgttt taaaattaca atccagcatc aaaaaaacat atgaagctat
      361 ttcttcggag gaacaaattg taatttacgg tcaagaaggt catgcagaag taagaggatt
      421 ggtaggacaa accaatgata atgctattgt tgtagaaaat gaagaagatt tgaaaaaaat
      481 tgattttact aaaaaaattc atttattttc gcaaactacc aaagatttgg aaagttttaa
      541 ccatttggca acaactattt cagaaaaaat aaagaaaaaa gaaaatttaa aaataagcga
      601 tacaatttgt cgtcaggtgg caaatagaat cccaaatatt actaattttg cacagcaaca
      661 tgatattatt ttatttataa gtggcaaaaa aagttctaac gggaaagtgc tgtttgaaca
      721 ttgtaagaaa gtaaatccca atacttttta catttacaat tgtgatcaaa ttgatgattt
      781 gccaattgac cttacaaaaa aaataggtat ttgtggggca acttctaccc ccaaatggtt
      841 gatggaatgt gtagcagaaa aattaagaca aaaagttaat taacgatttt aaagaaatta
      901 attgaaaaac ttatcataat ggaatatagt attaaatgta tcggtttact tacttctgga
      961 ggtgatgctc cgggaatgaa tgctgcaatc cgagcagtaa cacggactgc tatttttaat
     1021 ggaattcgta taaaagccat ttacagagga tacagaggac ttattacggg tgaaattaaa
     1081 gagtttaaga cagaaaatgt aagtaatatt attcaacaag gaggaactat tttaaaaact
     1141 gcccgttgtg atgaatttat gacacttgaa ggtcgcaaat tagcttacga aaacatgcaa
     1201 aaagaagaga ttgatgcttt aattgttatt ggtggtgatg gaacttttac aggtgctcgt
     1261 atttttgctc aagaatttaa tgtcccgatt attggaattc caggaactat tgataatgat
     1321 ttatatggaa cagatgtgac aattggtttt gataccgctt taaatactat catagaagcg
     1381 gttgataaga ttcgtgatac tgcttcgtcg catgaacgtt tgtttttcgt tgaagtgatg
     1441 ggacgcgatg ctggttttct ggctttaaat agtgcgttgg cttcaggagc agaagctgcc
     1501 attattccgg aacgggaaac aaaagtagat caattggaag aagtaattaa aaatggtttc
     1561 agaaaatcca aaaatagcag tattgttatt gtaactgaga gcaaagtgac tggtggagcc
     1621 aacggtttgg ctacacgtat gcgaaaagaa catcctgaat atgatgtacg agtaactatt
     1681 ttggggcata ttcagcgtgg aggttctcct tccgtttccg acagaatttt ggcaagccga
     1741 atgggagcgg ctgccgttga agctttactg gacgaacaac gcagtgttat gattggtgtt
     1801 gttaataatg aaattgtaaa tgtgccgttt acaaaagcta tcaaatataa taaacccata
     1861 gatgaagatt tgctccaaat tattcaggtt ttatccattt aatagggaag gatggactcc
     1921 aaacaaaata gaaaatcgca tattgttttt ccatgaaact ttatgcgatt ttttattttt
     1981 caggttcatt ccagattttc caggctgctt cggcttgttc ctccaacatt ttttcgccat
     2041 ttactccttt ggctccttga agttttcctt ttcttaggaa taatgtttca ggaggattat
     2101 aaacgacatc aaaaagtaag tgctcatttg tcagaaatgg ataaggaata gctggacatt
     2161 catcagcatg aggaaaagta ccaagaggag tagcattgat aataagtttg ttttcccgaa
     2221 ttgtttgatt atcaagcatt tcgtaagaaa gcattccttt gcaagggcga cgtgaaacaa
     2281 aagtagtttc aatcccaagc ttttgaagga caaaataaat ggcttttgaa acgcctcctg
     2341 tacctaagat caaggcttta cgatggtagg gctgtaaaaa aggaaggatg gatttttcaa
     2401 aacctatcac atcggtgttg taacctttta attttaagat tttaccattg cgaatgaatt
     2461 ttattacatt tacagctcca atttcttttg cagtatcatc cagctcatca agataaggaa
     2521 taatttgttc tttataagga atagtaacat tcaaaccagc aagttcagga atatttttta
     2581 attcaggaaa taaatcgata ttttctaatt catacaaatt atatcgagca tcaatatttt
     2641 ctttttcaaa tttttcagta aaatattttt ttgaaaaaga atgagtcaat ggataaccaa
     2701 ttaatccgta cagtttcatt ttttctttag cctcaaataa tatttaattc caccaaaaat
     2761 ccctgctgca actactaata ttaccataac aatactgatt tctttgttgt atttattaat
     2821 caaatcggct tgtccatgag ccagatatcc aatataagcc agcacacaat tccatactcc
     2881 agcccccaaa acagtataca aagaaaattt aaaaaaattc attttagcta agccagcagg
     2941 gatagaaatt agttgacgaa tacctggaat taatcttcca ataaaagtgg aaatatttcc
     3001 atacttatta aagtaagctt cggctttacg aattttttca ctattcaaaa gtagagcagc
     3061 tccaacttta ctgtcagcca atttgtaaat caaagccctt cccaacgaaa tggaaagagc
     3121 ataattgata taagcaccca tcagtgaacc caatgttgca aacaaaataa ttaagaggtg
     3181 gtttaaatta ctatctggtt ggctagcgat ataagcagcc ggggggacaa taatttcaga
     3241 aggaaaaggg ataaaagaac tttcaatagt cataagagca caaatagcac tataagtcat
     3301 atttttgtta taccatcttt ctattttttg aagtaaagaa agttcttgaa ccgtttttaa
     3361 attactaata tttttttgag cttcagttgt ttgtatatta aatgataaaa aagtaaacaa
     3421 gaaaacaaga agaataatag gtttattttt cataaaatga tactaaatgt tcatattaaa
     3481 gaataggatc tattgcttcg ggacaaattt ccaaaaacat gctggaagct tcttcatttt
     3541 cttgaatggc ttttttcaag taagcataag ccagcgtttt attcccgatt ttgtaataag
     3601 taatggctaa aaacagataa agatattcta tggtagaatc ctgttgtaat acttgaagat
     3661 aaaattccag tgctgtattg taatcaccct tttccagatt gatattggca attatcatca
     3721 atgtttctgg ttgacttgga tctaatgtaa tagatttcaa ataacaaagc aaagcattgt
     3781 caatgtcgtc cattctcaaa taatcttctc ccagataatt ccaggcgtcc gtatcttctt
     3841 tattaatatg taacgctttg gaaatgaatt gtatgctttc tttgaatttt tctttttcca
     3901 ataagcaagc tccaatacct aacagtgcat cataattgtc aggttgaatt ttcagggaac
     3961 gttggtaata agaaattgca tcgtcaaatt gatccatttt ctcgaaactt tctgcaatat
     4021 aaatattggc ttgttcactg tcgcttgtca attcagcata ttctttataa ctttcaatgg
     4081 cttctttgaa ctgatttagt tgaaaatggg catgagcttt ttgcaaacaa gcaagagaat
     4141 cattttcatc aattgccaga acaaaatcat acgcttcaat agcttttgaa aattcttgag
     4201 aagtaaaata aatttgtccc aaattgaacc atgcttctga agaataagga tcgaggtcaa
     4261 tgattttgtt gtagatcact attgcttttt cgtcttcgtc caattgttca tagcagaaag
     4321 ccagttcaaa taacaagtcg ctatttttag gattgatttt ataacctttt agcaaccatt
     4381 ccattgcttc gttgaaatat ccgtgctcaa tataaatgta ggcaatgtcg agaaggacat
     4441 cgtcttttcc ttcattttca aaatcaatta cgcggttaca taattgataa gcttcggata
     4501 aacgtctcat atgcaccaat gcttggattt taagcaaaat aacttcgtaa tcatttttat
     4561 cagcaagcga ctccaaaagt tgataagctt tctctgcatt tccagaagca agatatattt
     4621 tagcccgttt agtttttaat tcgctgctgg tgggatgaag cttaattcca aattctatag
     4681 ctctggaaga atctttagta cgaccttttt gtaaataata ttctgcgata ttttcgaatt
     4741 cttcaatgtc aaaataacct gatgcttttc cagataaaaa ttgttcataa cgatgaatta
     4801 tctcattgat gtcatcatcg ttttgaaaaa atatttttct gctcatgctt tatttttttg
     4861 caaaattaat attttaattg taataaaata aacctcttaa ctaaaagttt taaacaatcc
     4921 cgtatttctt ttcgataaag ataatatact gttaaaaggt ttttttcgtt ttaccaatag
     4981 aaacacttat ggcatattta ttgtttactt tgcactattg tttgtcgttt ttaatatcaa
     5041 ttcaagttaa attttttcat ctgttggaca attacaagtc attttgtttt taattacttt
     5101 actttgagac acttatatgg atttttttga aaaatttcag ttttgtcctg cttgtggttc
     5161 accttttttt ataaaaaaca atgaaaaatc aaaacgttgt cagcaatgtg attttgtttt
     5221 ttacatgaac gcttcggcag cagtagctgc ttttattttg aatgaaaaaa acgaattact
     5281 ggtttgtaag cgaaaaaaag agccagccaa aggtacatgg gatttgccag gtggttttgt
     5341 ggataacaat gaaactgctg aggcagccgt aaaacgggaa cttgcggaag aattacacgc
     5401 agaaacggtc caggtggaat atcttttttc agaacccaat gaatatgaat acagtgattt
     5461 agtggttcca actttagatt tattcttttt gtgttgttta aaagatgaat cccagcttat
     5521 tccttctgat gatgtggaag attgttttta tgtagcatta gataaacttg agccagatta
     5581 tttcggcttg aaatccataa gaaaagcaat aattcggttt attgaactta ctttgggaaa
     5641 agctgaatga aatatttaat tttgctttgt caatggtaaa aataattgta atgaaattta
     5701 tttaaatcaa aaattgaagt gagaatgaaa aaaagttttt tccttttcag ttttttgtgt
     5761 gtgataacaa ttcctctgct gaatgctcaa tatactttga tttatactga tcctaatgct
     5821 tctttcgaca aagggaaaga gctttatttt caacgaaaat atactgcatc ttatcattat
     5881 tttgaagatt ttcttaaaac agcaaaacct cagcaagccg ggcagatgca tgaagcggaa
     5941 tattttttag ctgccaatgc tttcgagttg cggcagaaaa acgcaatgga attgttaaat
     6001 aattttttgt tgaaatatcc ttatacttct tatgctgatc ctgtctatgc catgataggc
     6061 atgctttatt atgaacagaa aaattatagc gaggcaatta tctattttga aaaagtaaaa
     6121 gaaaataatt tgaatcctga taaaagaata gatttcttat tctgtaaagg ttatgcttat
     6181 cttgaaaaca aagagtataa aaaagcaagt gatattttta aggatttaaa atctgttgaa
     6241 tcgccccaaa aaatttctgc tatttattat tatgcttatt cacaatattt gttaggaaat
     6301 tatgaagagg ctttgcctga atttttgaaa atcaaaaatg aaactgatta tcaggcaatt
     6361 gttccttatt atttggttca aatttattat attcagaaag attataagct ggcaagacaa
     6421 gaagcttttt ctttattaga aaattatcct gataacaaaa ataacgctga aatttatcga
     6481 attctgggtg aaattgctta taatgaaaaa aattataagg atgccgtatt ttatttaacc
     6541 aaatatgaaa agatgtttcc tcaggtatta aggaatgata tgtatatttt aggtttatcg
     6601 tattttcaat tgaaagatta caaaaatgct gttgtttatc tgtcaaaagt tactactaca
     6661 cgggatgaaa tttcagaaaa tgcttatctt tatttaggaa gttcctatat ttatttaaat
     6721 gacaaaatca atgctcgatt ggcttatgaa gcagcattag aaacaaactt caatcctgcg
     6781 gtgagagagg aagccttgtt taattacgct ttgacgtgtt atgaaactcc ttctgccttt
     6841 ggggaaacta tttcagcttt tactcgcttt ttatcagaat ttccaaactc aaaatattca
     6901 gacgaagctt atcgttatct tgcggaagca tatcttacta ccaaaaacta tgaattagct
     6961 tatcagtcca tttcccaaat caagcaacta atgcctcaat tatcagaaac caaacaatat
     7021 ttgctttata agcttggaag tcaagctttt gttcagaatg attatgaaaa agcaatagat
     7081 tattttactc gtgctctgga aatttcttct tccaataaat atgctgctga atgtttgttc
     7141 tggcgagcag aatgtttttc ccgtcttcaa aaatctgccc aagcccaaaa tgatttgact
     7201 gcttttttca aaaatgcgta tgcaaaatca agtgtaaatt ttataatggc aaattattca
     7261 atggcttatg cttatttttc gcagaaaaaa tattctgaag cgctgaattg gttcttaaaa
     7321 tatataaatc ttgaatcaaa ttcttcttca gtgacttatg ttgatgcatt gaaccgaatt
     7381 ggtgattgct attataatgc tcgtaatttt gaacaagctc taaaatatta cgacaaagta
     7441 gttcaactaa gtcctgaaac agccgattat gcattatttc aatcagctta tattgaagga
     7501 attaaaaaga attacactgc caaaatttca aaattggaaa aactgttgac actttatcct
     7561 ggttctgaat ataacgataa tgctttgtat gaaatagcac gttcttactt gatgcttaat
     7621 aacaatgaaa aagctatagc tatttatcag cgattgataa atcaattccc aaattcagtt
     7681 ttggctcgga aatcaatgct ggaaataggc atgatttatt tgaatgagaa aaactatgat
     7741 aaagcaataa ccacttttaa gaatgttatc gaaaaatatc ctgaaagcga ggaattttat
     7801 acagcgttgg aaagtttgga aaccatttat gtagagataa acgatgtgaa tgcttatttg
     7861 aactatgccg ctagcttggg acaaaaaata aaaaatgttg ccgaacaacg tgaagattct
     7921 atttcttata tagctgctga gaaacaatat ttgaatggaa aatacgaagt agcattgact
     7981 ggtttgtcca gttatgtgaa caagttttgt cctggaggac gacattgtat cattgccagg
     8041 tattatcttg ctgattgtta ttatcaaact ggtgaaaaag aaaaggctct atccacttat
     8101 caagctttgc tggagttgcc cgtaagtgaa tttacagaag aagctgcttt acgttgtgct
     8161 gaaattactt atgataaaaa agattattcc tctgctttga attattttaa aaagctggaa
     8221 acagttgctc aatcgaccat taataagaat gctgcacgtt tagggatttt acgatgtaat
     8281 tattttttaa atgattatca atcgctggtg aacattgctt ctgaaataat tgccgatcca
     8341 cgttctgatg agagtttatt aatcgaatcg ctttattatc gtgctaaaag ttatcttgaa
     8401 cttggaaaaa atgaacaggc aattgttgat ttggaaaaac tttctgttga tacacgaaca
     8461 acttggggtg cagaatcaaa atatttactg gcaaatgtgt attttgaaca aggtaaactg
     8521 aaagaagcag aaaatgaaat tatggatttc atgaaaaaaa atacgccaca tcaattttgg
     8581 ctagcaaaga gtatcgtgct tttagctgat atttatgtga aaaaaggaga tgattttcag
     8641 gcaaagcaat atttgttaag cttacgtaaa aattatctga atcatgatga aattgaaaat
     8701 cttattgagg aacgtttaca ggcaattgcc aaacgagaaa agcagaaaat cattggctaa
     8761 attaatttaa gaaattttgt tcatcaattt tagaaaaatt cttcaaatga atagaaaaaa
     8821 attattgacc tttattttca tagttgcaat aaatttcatg tctgtttttg ggcaagattc
     8881 tattttcaga cgtgaagtta cagtggaacg ggaatatgtg cctgtcatta gagaagcagg
     8941 aaaaatcaat ttaactccta aattgtatga gccacaagtg gaaaaattat cgccagttta
     9001 tacggaattt tatttgcctt tacctgtgga aaccaacatt catcctttgc aggcaattga
     9061 attttcttta caacgagaac ctctaaaaaa cggctataca aaaatcggtt tgggaaatta
     9121 ttataattct ttgttggatt ttgcctatcc atttataaaa aaggaagata tgtatttgga
     9181 ttttatagtt catcatctaa gtacgtatgg aaacaaatgg catactactt cggatgcgtc
     9241 acttttcttt aataaagatt tcaaaaatct tgattttttc actggtgtca atgccagcta
     9301 tgagcgatta aaatattacg ggaataattt taatcttgat tctatagttg atttaaaaca
     9361 attggccgga aattatcctt tggctattta tttaccaacg gataaagaac aacccatttt
     9421 ttcttattct ctgaaagaat tgtctgaact gaatcgattt gacactttct ggagaggaaa
     9481 tatttatgga ggtgttcaat caaaatcgaa tgttactgat attcaatatg ttggcaaatt
     9541 agattattct tttttaaatg ccagtagcgg aagcaaagaa catcagattt tattaagtgg
     9601 caaattccag ataccttaca atgacaataa attagggttg gatgctgatt tgtttcaatt
     9661 aaattacaat tcgaaagtaa cggattcaac gaatatagaa aattcctatt cggtttttat
     9721 cattcatcct tattatgatt atcactccga taaatttaga gctcgtttag gaatacaatc
     9781 agctttcagt tttgaagaaa aaggttttct tcatctttat cccgatatac aagcggaatg
     9841 gcagccattt ccaacatgga tggcaattta tggcggaata actggaaatc atcaagttaa
     9901 cagtaggagt aaaattttaa aggaaaactg ttttgctgag gcttatttaa aagtaaatga
     9961 tacttttact ccttttgaag gttatcttgg aattaaatta aaacctgctg aactattttt
    10021 tgtggatgct tttattgatt atcaacatat taataatcaa tattttttta tcaataaaga
    10081 atttctctca aatgatctga gagaacccaa taatcatttg tttatcaatc gttttgatgt
    10141 aacttacagt aaaactgatc tttttaagag tggtttgcgg ctttcatatc atttgcagga
    10201 tcgtttcagt tgtgaattga aaggtgttta taatgattgg caagtttccg atgaacaata
    10261 tgcctggaac aaaccaaaat gggaagctga tatgaacttt caggcttcat tgactgctgg
    10321 tgtaaattta tcagctaaga ttttttatca aggtggtcgt tatgcaaaat taggtgaaaa
    10381 agcaatttca atgaaatctg cttttgatgt taacatgggc atttcttata attattcccg
    10441 ttttttgact gtttttgcaa aagtgaacaa ctttttcaat agcacttatc agcaatttta
    10501 cggatacgat gttcaaggaa caaatttttt gctaggagct gcattgtctt tctaaattag
    10561 ttaatttttt taacaaataa gagcatttca tatcttttat ttatgattag caaggaagaa
    10621 gtttttccaa ttggtgagat tataaagcct cacggcgtaa aaggcgaaat gtccttttta
    10681 ttaaaacatc ccatttcaga ttgggagaaa attccttttt taatctttag tagggatggt
    10741 attttagttc ctttttttat tgagaatatt cgatggaaat ctgacaacgg tggttttctg
    10801 aaattggaag acataaatac agaaacagca gcccataaat tttctggatt gactatcttt
    10861 ttacataaaa aattcttaaa tcatgtcatt tcgttggaag aagaagaatt gcaagcagaa
    10921 tattttattg gttttcaagt aataaatcag gaaaatgttt tgattggaac cattcaggaa
    10981 gtggatcaaa gcacggaaaa tattttgttt gtagttgaaa aagaaggtca ggaatatttg
    11041 attccattaa gcgaagaata cattaaaaat attgatcaca aaaaaaagga aatttttatg
    11101 gagttgcctg aaggattgtt ggatttataa aatacctaaa atccgcaagt caattatttt
    11161 gaaaaaagag ggcatatttc agcggacgt
//
LOCUS       DAOSAS010000049        11130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3902_length_11130_cov_13.001174, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000049 DAOSAS010000000
VERSION     DAOSAS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3902_length_11130_cov_13.001174"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..>2968
                     /gene="purL"
                     /locus_tag="PLR72_03580"
                     /gene_synonym="purI"
     CDS             6..>2968
                     /gene="purL"
                     /locus_tag="PLR72_03580"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOK36322.1"
                     /translation="MILFFQASDNQIYAVQSAVELTKEDFQKLIWLFGEAKLLSETQI
                     EGNFIGPRREMITPWSTNAVEITQNMGITGILRIEKFRQVSFAEKDKDSEVYDPMLER
                     VYSKLDQHIFTIDKTPDPIIYIDDIAAYNEKEGLALSEEEIAYLNEVAKKIGRKLTDS
                     EVFGFSQVNSEHCRHKIFNGKFIIDGEEKEMSLFQLIKKTSKENPNFLISAYKDNVAF
                     NQGPEIQQFSPEKGDQPTFFQVKNISSVLSLKAETHNFPTTVEPFNGAATGTGGEIRD
                     RLGGGKGSLPLAGTAVYMTSYPRNSEETVSDKKSSFGNDTEQKFSVFSKNWERAIQPR
                     KWLYQTPEQILIKASNGASDFGNKFGQPLICGSLLTFEHEENGKKYGFDKVIMLAGGV
                     GYANQRDALKQKVTAGDKVILMGGDNYRIGMGGGAVSSVETGQYDNAIELNAVQRSNP
                     EMQKRVANVIRSLTETERNPIVSIHDHGAGGHLNCFSELLEETGGKINISALPVGDPT
                     LSAKEIIGNESQERMGLLLKEEDIEEVRKISERERSPFYVVGEATDNKQLVFYQEDGQ
                     KPIDLKLEYLFGNPPRTVIVDETIEEQFAAIEIQEDKILEYLQNVLQVEAVACKDWLT
                     NKVDRSVTGKVARQQCLGEIQLPISDVGVMALDYEGAKGIATSIGHAPLAALVDPQAG
                     SVLAIAEALTNIIWAPLVNSLKSISLSANWMWPSKNPGEDARLYKAVEACSDFACSLG
                     INIPTGKDSLSMTQKYGDEKVFSPGTVIISAAGEVSDVKKTVSQVIVNEKDTFIYYID
                     FSKDDFQLGGSVLAQTLNKVGDKVPTIKNAQYFVKAFETIQQLINEDLILAGHDISEG
                     GMIVTLLEMCFPNLNGGLKVCLDGISEKSIVKILFAENPGILIQIKNKEAVDQILKEN
                     KIRYVHIAQPIAERRLEIQQGKISYAFSINELRDVWFHSSYLLDRKQSSEICAQERYM
                     NYKKQPLEFSFLPSF"
     assembly_gap    2969..3068
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3069..3293
                     /locus_tag="PLR72_03585"
     CDS             <3069..3293
                     /locus_tag="PLR72_03585"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOK36323.1"
                     /translation="YIYSEYPGNPNGSDYSVAAICSADGRHLAIMPHPERAIFPWQWA
                     HYPADRKNRDEISPWLEAFVNARKWIEKRK"
     gene            3430..3864
                     /locus_tag="PLR72_03590"
     CDS             3430..3864
                     /locus_tag="PLR72_03590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK36324.1"
                     /translation="MENITVNSPVRFAKKEDIDAIHHLLDMEPFKYDNSLPYQRSWIK
                     QLIMNKRCITLVYETEGIIKGFISGEKLVSGSVMIWFCAVKKEYQHRIIGGRLYFEFE
                     NVCKQIGIKGILAYGYKTSVNMLRKLQFFTNEKKYLEFYKVL"
     gene            complement(3894..4988)
                     /locus_tag="PLR72_03595"
     CDS             complement(3894..4988)
                     /locus_tag="PLR72_03595"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOK36325.1"
                     /translation="MISLKSNAKNILFFLFVFFSQFLQAEIPEGYYLSAEGKTGAELK
                     TALHNIIRQHTILNYDTSTSVWWDTYFKTTDWHPEGYFWDMYSNNKRSTYNSSLMNRE
                     HCMPRSWWATSDNYSSYDANGDLVNLSPADAEANTKKSNYPLGEVGKNSVTYTNGVVK
                     VGANSFPGYNGTVFEPADEYKGDFARTYMYMVTCYEDYANNWRGTGTASMLQKNTYPV
                     FKSWAIELLLKWSTNDPVSQKEINRNEGVYQIQKNRNPFIDYPELAEFIWGTKNSEQW
                     QPEIGTSTFVVSPNPASSYVSVQISQPEQATFIIYDLSGIRLKNGKINSDGTIYIGDL
                     NNGIYLLTIYAQNTRTVTKLIVTHEKLIQN"
     gene            complement(4998..7823)
                     /locus_tag="PLR72_03600"
     CDS             complement(4998..7823)
                     /locus_tag="PLR72_03600"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOK36326.1"
                     /translation="MSKKQTIIVLFFLLLTVVAFGAIPPGYYYFARNKKKAELKTALA
                     QIAAPLKVLKYGSGPGYTWEGFYYTDQNPDGSVIDMYSPKVRYFNGFSSVNGMHIEHS
                     LPKSWWGSIENNAYKDLFHLYPADGETNSIKNNFPLGEITIPIFDNGVSKIGKNSFGD
                     FYTDNCFEPADEYKGDFARSYLYVSTIYENFAPLWNSPMMDNNTYPVWKPWAMDLLLK
                     WHREDPVSEKEEKRCEAVYNIQGNRNPFIDYPELVEYIWGKDTTNIFPFPEETEAFLL
                     SPRKGSEIDFGVLLQNDTKTLEILIQGINITSEVKISLIKKNSSFHLSSSTISKDEIL
                     NGTNILLTFQPQTSGWFNDTLSISEGGLKENLYIPLKALASPDFITLEPDDVTPVGGI
                     LRWIYDPQAIDYLLDVYQGDAQAGDLIISGYAEGSSWNKAIELFNGTGKPIDLSKYSL
                     QKQTNAYGPFGSNFKLNGILQNGKTYLLVRKDANDVLRNKANILTDSLLAFDGNDPIA
                     LVRNGIIIDMIGDPNLGGNVYWGQDVTLKRKSYVTHPISTCNFAEWDRYEKDDFTKLG
                     SHHMDFQTTKSYVLQNYSTGGDAEFIIDQLMPESTYTYSVKAIRSNEEIVSANTMQIH
                     TTRLDMPIAMDALNVHSTGFIANWEDHLYTQNFLLDVFQLQETADTTEIETFDNVGSN
                     GKPLPTGWTGTASGNYTSSISSGETPPSIALKNDGEYLQTKTYPLPVKNLSFMYRFPS
                     EATGSSLILEGIKENGNISRVDSIPYVNSSKNYPMYFFQPSDEIVAFKFTYRKSKGNF
                     ALDDVAVTYRKQDTIYVLKNEPVVGSQFEVDNLNENSTYYYRVRATLGNSVSDFSDVI
                     EVQTTNESGIKHYEDFPFQIIPESDQIRIKGLQGDEQIQIYAISGICLHLTHVNSTEV
                     SIPLKQKGIFIVRILNHNKVFVKKIVK"
     gene            9078..10217
                     /locus_tag="PLR72_03605"
     CDS             9078..10217
                     /locus_tag="PLR72_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOK36327.1"
                     /translation="MKKIIFLFTISLFLVSCHSHDFFKVQGTVTGAKGEKIYIEHNSL
                     TKVIPLDSVKLSSKESFSFKIHRPEYPDFYRLRLNNQFIVFAVDSCEEITIHADSKSF
                     SKNYTITGSEPSKQIQQLHLSLMKIQEKINTLSSNLPPERKDSFISEIKNDIEKHKEL
                     AKNIIFQNPRSTAAYYALFQKLNNTYLFSPYVKEDRPYCAAVATSYHTFMPEYERSKN
                     LYALVMDAITKERKAIKNEEWEKVLEKNGKGYIDIVLKDSKDKIRKLSELEGKVVLID
                     FSTYETKESIEYIFALRDLYNKYHSRGFEIYQVSLDQNKDRWKQAVENIPWICVRDEN
                     GYQTPYVLSYNVTFIPTNFLMNKKGEIIARDLDFKQLNQMIDNLL"
     gene            10270..>11130
                     /gene="queG"
                     /locus_tag="PLR72_03610"
     CDS             10270..>11130
                     /gene="queG"
                     /locus_tag="PLR72_03610"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOK36328.1"
                     /translation="MIKDFIKETAASLGFDACGIARAEELKEDADFLKGWLKAGYHGE
                     MYYMEQNFEKRINPQKLVPDCKTVVVTLLNYYIDCPSPTDAPRIAKYAYSETDYHKVI
                     KQKLRQLENEIKNKFGENCFSKDFQHVFVDSAPILERRWAERAGLGWLGKNTQLIHPG
                     LGSYVFIGILLLNVEVEPDLPISSHCGKCKLCWEACPTKALSENGLDARKCISYLTVE
                     YKKEIPPDFQNCLAGFIAGCDICAEVCPWNKKWAVSHNHDELKPNSHILNWKYENWEH
                     LSPADFEEIFH"
BASE COUNT         3838 a         1718 c         1915 g         3559 t
ORIGIN      
        1 aaaatatgat tctttttttt caagcttctg ataatcaaat atatgcagtt caatctgctg
       61 ttgaactaac aaaagaggat tttcagaaat taatctggct tttcggtgaa gcaaaattat
      121 tgtccgaaac acaaatcgaa gggaatttta ttggtcctcg tcgtgaaatg attacgccct
      181 ggagtacaaa tgctgtagaa atcacccaaa atatgggtat cacgggtatt ttacggattg
      241 aaaaatttcg acaagtgtct tttgctgaaa aagataaaga tagtgaagtt tatgatccta
      301 tgctggaaag agtttattca aagcttgatc aacatatttt taccattgat aaaactcctg
      361 atcccattat ttatattgac gacatagctg cttataatga aaaagaagga ttggccttga
      421 gtgaagaaga aattgcttat ctaaatgaag ttgctaaaaa aattggacga aagttgacag
      481 atagtgaagt ctttggtttt tcgcaagtaa attctgagca ttgccgtcat aaaattttca
      541 atggaaaatt tattatcgat ggagaagaaa aagaaatgtc gttgtttcag cttatcaaaa
      601 aaacttctaa agaaaatcca aattttttga tttcggctta caaagataat gtagctttta
      661 atcaaggtcc tgaaattcag caattttctc ctgaaaaagg agatcaaccg acttttttcc
      721 aagtaaaaaa tatttcatca gtactttctt taaaagctga aacacataat ttcccgacta
      781 ctgtagagcc ttttaatggt gctgccactg gaaccggtgg agaaattcgc gatcggttgg
      841 gtggaggaaa aggtagttta ccacttgcag gaaccgcagt ttatatgacc agttatccac
      901 ggaatagtga agaaacagtg agtgataaaa aatcttcttt tggaaacgat acggaacaaa
      961 aattttccgt cttctccaaa aattgggaaa gagctattca acctcgcaaa tggttatatc
     1021 aaacacctga acaaatttta atcaaagctt cgaatggtgc ttccgatttt ggaaataaat
     1081 tcggtcaacc tttgatttgt ggctcattgc tcacttttga acatgaagaa aatggtaaaa
     1141 aatatggttt cgacaaagta attatgctgg ctggtggtgt tggttacgcc aatcaacgtg
     1201 atgcattgaa acagaaagta acggctggag ataaagttat tttgatgggt ggtgataatt
     1261 atcgaattgg gatgggaggt ggtgctgttt catctgttga aacgggacaa tatgataatg
     1321 cgattgaatt aaatgctgtg caacgttcca atcctgaaat gcaaaaacgt gttgccaacg
     1381 ttattcgttc tttaacagaa actgaaagaa atcccattgt gtccattcac gatcatggtg
     1441 ctggcgggca tctcaattgt ttttctgaat tgttagaaga aacaggcgga aaaataaata
     1501 tttctgcatt gcctgtggga gatcctacgt taagtgcaaa agaaattatt ggaaatgaaa
     1561 gtcaggaacg catgggttta ttgttgaaag aagaagatat tgaagaagtt cgtaaaattt
     1621 ctgaaagaga acgttctcct ttttatgtag taggtgaagc cactgataat aaacagttgg
     1681 ttttttatca ggaagatgga caaaaaccta ttgatttaaa actggaatat ttatttggga
     1741 atccacctcg tacggtgata gtagacgaaa caatagaaga acaatttgca gctattgaaa
     1801 ttcaagaaga taagatatta gagtatctac aaaacgtgtt acaagttgaa gctgtcgctt
     1861 gcaaagattg gttgacgaat aaagttgacc gttccgttac aggaaaagta gctcgacaac
     1921 aatgtctggg agaaatacaa ttaccaataa gtgatgttgg cgtaatggca ttagattatg
     1981 aaggagcaaa aggaatagct acttctattg gccatgctcc actggctgca ttggtagatc
     2041 cacaagctgg ttcagtgttg gcgattgccg aagctttgac caatattatt tgggctccgc
     2101 tggtgaatag cttgaaaagt atctcattga gtgctaactg gatgtggcct agtaaaaatc
     2161 caggtgaaga tgctcgactt tacaaagcag tagaggcttg cagcgatttt gcttgttcgc
     2221 taggaattaa tatcccaacc ggtaaagata gtctttcgat gacacaaaaa tatggtgacg
     2281 aaaaagtttt ttcaccgggt acagtcatta tttctgccgc tggagaagtt tcagatgtaa
     2341 agaaaactgt ttcacaagtg attgtaaatg aaaaagatac atttatttat tatattgatt
     2401 tttcaaaaga tgattttcaa ttaggcggtt ctgttctggc acaaacactg aacaaagttg
     2461 gtgataaagt ccccaccata aaaaatgctc aatattttgt taaagctttt gaaaccatcc
     2521 aacaattgat aaatgaagat ttaattttag caggtcacga tatttctgaa ggaggaatga
     2581 ttgtaacttt gctggaaatg tgtttcccaa acctaaatgg tggtttgaaa gtttgtttgg
     2641 atggaatttc tgaaaaatca atagtaaaaa tattatttgc tgaaaatcct ggcatattga
     2701 ttcagattaa aaacaaagaa gctgttgacc aaatattgaa ggaaaataaa ataagatatg
     2761 ttcacatagc tcaacctatt gcagaaagaa gattggaaat acaacaaggg aaaatttctt
     2821 atgctttttc tatcaatgag ttgcgagatg tttggtttca ttcatcgtat ttacttgatc
     2881 ggaagcaaag cagtgaaatt tgtgctcagg agcgttacat gaattataaa aaacaaccgt
     2941 tggaattttc ttttcttcct tcttttcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnta tatttattcc gaataccccg gaaatcccaa tggatcagat tacagcgtgg
     3121 ctgctatttg ctctgccgat ggacgtcatc tagccataat gccacatcct gaacgagcaa
     3181 tatttccgtg gcaatgggca cattatccag ccgatagaaa aaacagagat gaaatttcac
     3241 catggctgga agcttttgtc aatgccagaa aatggattga aaaaaggaaa taaattttat
     3301 tgatgaagtt ttaaaatttt tccttctgaa aaatttttga ttacacttga aaaaatcaat
     3361 atacctcagg cttatttgat aattttttaa aatttagctt gtgagtcaaa aaattatgaa
     3421 atacaattca tggaaaatat aactgtcaat agtccagttc gttttgctaa aaaagaagat
     3481 atcgatgcta ttcatcactt gttggacatg gaacctttta aatatgacaa cagcttacca
     3541 tatcaacgaa gttggataaa acagttgatt atgaacaaaa gatgcattac tttagtgtat
     3601 gaaaccgaag gaataatcaa aggttttatt agtggtgaaa aattggtaag tggtagtgta
     3661 atgatatggt tttgtgcagt aaaaaaggaa tatcaacatc gtattattgg tggcaggttg
     3721 tactttgaat ttgaaaatgt ttgtaagcaa attggcatta aaggaatttt agcttacggc
     3781 tataaaacgt cagtaaatat gcttcggaaa cttcaatttt ttacaaatga gaaaaaatat
     3841 cttgaatttt acaaagtatt atgaaaaaag ttaaaaaaga aaaatataaa ttatcaattc
     3901 tgaattaact tttcatgagt aacaattaat tttgtcactg ttcttgtgtt ctgagcataa
     3961 atagttaata aataaatacc gttatttaaa tcaccgatat agatagttcc atcactattt
     4021 atttttccat ttttcaaacg gattccactt aaatcataaa ttataaaagt ggcttgctcc
     4081 ggttgactga tttgaacact cacataagaa gaagcaggat taggtgaaac aacaaaagtc
     4141 gaagttccta tttccggttg ccattgctca ctgttttttg ttccccaaat aaactcagca
     4201 agttcaggat aatctataaa aggatttctg tttttttgta tttgataaac accttcgttc
     4261 ctatttattt ctttttggct aactggatca ttcgtgctcc attttaacaa caactcgata
     4321 gcccacgatt tgaaaactgg ataggtattt ttttgaagca tactggctgt tcctgttcct
     4381 ctccaattgt tagcataatc ttcataacag gtaaccatgt acatgtaagt tctggcaaaa
     4441 tcacctttgt attcatctgc aggttcaaaa acagtaccat tataaccagg aaaagaattt
     4501 gctccaactt taacaactcc attggtatag gtaacactat ttttccctac ttcacccaaa
     4561 gggtaatttg attttttagt gtttgcttcg gcatcagcag gtgagagatt taccagatcg
     4621 ccattggcat cataagaaga ataattatcg cttgttgccc accagcttcg tggcatgcaa
     4681 tgttctctgt tcattaaaga agaattgtag gtagaacgtt tattattaga atacatatcc
     4741 cagaaatagc cttcaggatg ccaatcggtt gttttaaaat aggtgtccca ccacacagaa
     4801 gtactggtgt cataatttaa aattgtatgt tgtcttataa tattgtgcaa agcggttttt
     4861 aactcagctc ctgtttttcc ttcagcagat aaataataac cctcaggaat ttcagcttgt
     4921 aaaaattgag aaaaaaagac aaaaagaaaa aacagtatgt ttttagcgtt tgattttaaa
     4981 gaaatcataa gaaaaaatta ttttactatc ttttttacaa atactttatt atgattcagt
     5041 atacgaacaa taaaaattcc tttctgcttc aatggaatac ttacttctgt agaattaacg
     5101 tgagtcagat gtaaacaaat tccagaaata gcataaattt gaatttgttc atccccttgt
     5161 aatcctttta ttcttatttg atcgctttca ggtataattt gaaaaggaaa atcttcataa
     5221 tgtttaattc ctgattcatt tgtagtttgt acttctatta catccgaaaa atcagaaaca
     5281 gaatttccaa gcgttgcacg aactcgataa taataagtgg aattttcatt cagattatca
     5341 acttcaaatt gactgccaac cactggctcg ttttttaata cataaatggt atcctgcttc
     5401 ctataagtaa ctgctacatc atccagggca aaatttcctt ttgattttcg ataagtaaat
     5461 ttaaaagcaa ctatttcatc agaaggttga aaaaaataca tcggataatt tttagaactg
     5521 ttaacataag gaatactatc aacacgacta atattaccat tttcttttat tccttccaaa
     5581 ataagagaag aacccgttgc ttcagaagga aaacgataca taaaggaaag attttttaca
     5641 ggtagaggat aggtttttgt ttgcaggtat tctccatcat ttttcaaggc aatagaagga
     5701 ggcgtttctc ccgaactgat agaagacgta taatttccac ttgctgtgcc tgtccaacct
     5761 gtgggtaaag gttttccatt acttcccacg ttatcaaaag tttcaatttc agtagtatca
     5821 gccgtctctt gtaactgaaa aacatcgagt aaaaaatttt gggtataaag atgatcttcc
     5881 caattggcaa taaaaccagt tgagtgcaca tttaaagcat ccatagcaat tggcatgtct
     5941 aaacgagtag tatgaatctg catagtgtta gccgaaacaa tttcttcatt tgaacgaatt
     6001 gccttgaccg aataagtata agtgctttca ggcatcagtt gatcaataat aaattccgca
     6061 tcaccacctg tggaataatt ttgtaaaaca taacttttag ttgtttgaaa atccatatga
     6121 tgagagccca gctttgtaaa atcatctttt tcatatctat cccattcagc aaaattgcat
     6181 gtcgaaattg gatgagtaac atacgatttt cgtttcagcg tcacatcctg tccccagtaa
     6241 acattccctc ccaaattggg atctcctatc atatcaataa taattccatt ccttactaag
     6301 gcgatgggat cattgccatc aaaagctaat aaagaatccg ttaaaatatt ggccttatta
     6361 cgaagtacat cattagcatc tttgcgaacg agcaaataag ttttaccatt ttgcaatata
     6421 ccatttaatt tgaaattaga accaaaggga ccataagcat tggtttgttt ttgtaaagaa
     6481 tattttgata aatcaattgg tttacccgta ccattaaata attctattgc tttattccaa
     6541 ctgctccctt ctgcatatcc agaaataatc aaatcacctg cttgagcatc accttgataa
     6601 acatcgagaa gatagtcaat tgcttgagga tcgtaaatcc aacgcaaaat gcctcccacc
     6661 ggagtaacat catcgggttc taaagtaata aaatcaggcg aagccagagc ttttagagga
     6721 atataaagat tttctttaag tcctccttca gaaatggaaa gggtatcgtt gaaccaccct
     6781 gacgtttgtg gctgaaaagt gagcaaaata ttcgtaccat ttaaaatctc atcttttgaa
     6841 atagtggaag aagaaagatg aaacgaagag ttttttttta taagtgaaat ttttacctcg
     6901 gaagtaatat tgattccttg aataagtatt tccaatgttt tggtatcgtt ttgcagcagc
     6961 actccgaaat ctatttcaga accttttcgt ggagaaagta aaaaagcctc cgtttcttcg
     7021 ggaaatggga aaatatttgt cgtgtcttta ccccaaatgt attctaccag ttcaggataa
     7081 tcaataaaag gatttcgatt cccttgaata ttgtaaaccg cttcacatcg tttttcttcc
     7141 ttctcactta caggatcttc tctgtgccat tttaaaagta aatccatagc ccacggtttc
     7201 caaacaggat aggtattatt atccatcata ggagaattcc ataatggggc aaaattttca
     7261 taaatagtag aaacatataa atatgatcga gcaaaatcgc ctttatattc atcggcaggt
     7321 tcaaaacaat tatcggtata aaaatctcca aagctatttt tccctatttt gctcacacca
     7381 ttatcaaaaa tggggatagt tatttcacct aatgggaaat tatttttaat ggaattggtt
     7441 tcgccgtctg ccggataaag atgaaacaaa tctttataag cattattttc aatagaaccc
     7501 caccagcttt tgggaagaga atgctcaata tgcattccgt ttacactgga aaagccgtta
     7561 aaatatcgaa ctttaggtga atacatatca attactgaac catctggatt ttgatcagta
     7621 taataaaatc cttcccatgt gtaaccggga ccactgccat attttaaaac ttttaaagga
     7681 gcagcaattt gtgccagagc agttttgagt tcagcttttt tcttgttgcg agcaaaataa
     7741 tagtatcctg gtggaattgc accaaaagca actacagtta acaataagaa aaaaagtacg
     7801 atgatagttt gctttttact cattaatggt aaagtttcat tatattattg tcttttttca
     7861 ttgtaaaatg aagtatagca aaacaaaaca aggagaaaaa gaatatttca ataaaaaatt
     7921 tgattaattt tgaaaaaaat ttaaacgcaa aaattatgaa ataataagtt tattatccag
     7981 atacaataac aaatatacac atttgtgaga aaattaaaga aagttctttg ataaattttg
     8041 aataaatcta aatctttctt tcatcccttg ccgctattca taaatagaaa acaaagagaa
     8101 agttttgaga atcagaattg taaaaataaa taacgcccag tagtttttta aaagtaaaaa
     8161 ataatttatg gaactttcgt aagtaagata aggttttgaa atagaataaa agacagcata
     8221 gtttattttt taagtctccg aaattcagta atttatgttt cacaaaatga ataaccccga
     8281 ggcaagcctc gaggaattct tttgattaaa ataaaataaa aataatatgc taagagacaa
     8341 aaaagccatt aatactttta gaaataaaag actttttcac ttcaagtgaa aagtcaacaa
     8401 gaacaatatt aagtcccctg tgttctttga aattttctga caaacgattt ggtttacaga
     8461 aggaggtgaa ttaaaaatat aataattatg caaaattgag tgtgttattg ttttttccat
     8521 ttttcaacat aaaagtactt taccgttttc ttcctctgca cgaagtataa ctttttcctt
     8581 tcaaaacgac taaaagaggg aatggaagtg atgtcctaac cactgatata caaatcgaca
     8641 tttgaacaag tttctaagat ttattcaact cgttggtcaa gggaagtatt ttttaaagaa
     8701 agtaagtagt atcgtgaatt gggggaaatg ccaatcacaa gattttgatg tgcaggaaat
     8761 caccaccact ctttgaatgg tttgatacaa tttacttact ttggtaaaac acgattacga
     8821 aacgattaga gaaatttttc gagaaagtca gaaaaatact ttgcaattaa ccatagccaa
     8881 gaaattatga attattatca caaaattgct atttgtttgt ctaaaatttt tgaaataaac
     8941 aatgaaacat tgattaataa attatttttt aaatatgaag atattacaaa atatctaaat
     9001 ttgaaaaact ttctgcaagc gagtttgtta aagcttgcaa aaattgaata gttaaaattt
     9061 taaaaaaatt atagcccatg aaaaaaatta tttttctttt tacaataagc cttttcttgg
     9121 tttcttgcca ttcacatgat tttttcaaag ttcaaggaac agtgacaggt gccaaaggtg
     9181 aaaaaatata tatagaacac aacagtttaa caaaagtaat tcctttagat tcggtaaaat
     9241 taagttcaaa agaaagcttt tcttttaaaa ttcatcgtcc tgaatatcct gatttttacc
     9301 gtttaagatt gaacaatcaa tttattgttt ttgctgttga ttcttgtgaa gaaattacaa
     9361 ttcatgctga tagcaaaagt ttttcaaaaa attataccat tacaggttca gaaccatcga
     9421 agcaaatcca gcaacttcac ttgtccttaa tgaaaattca ggagaaaata aatactcttt
     9481 cttcaaattt accgcctgaa aggaaagatt cctttatttc tgaaattaaa aacgacattg
     9541 aaaaacataa ggaactcgca aaaaacataa ttttccaaaa tcctcgttct acggctgctt
     9601 attatgctct ttttcagaaa ttgaacaata cttatctttt ttctccttat gtaaaagagg
     9661 atcgacctta ttgtgctgct gttgcaactt cttatcatac ttttatgccc gagtatgaaa
     9721 gaagcaaaaa tttatatgcc ttagtaatgg atgccattac aaaagaacgt aaagcaatta
     9781 aaaacgaaga atgggagaaa gttttggaaa agaacgggaa aggatatata gacatcgttt
     9841 taaaagactc aaaagacaaa atccgaaagc tttccgaact ggaaggaaaa gtggtcttga
     9901 ttgatttttc cacttatgag acgaaggaaa gcattgaata tatttttgct ttacgcgatt
     9961 tatataataa atatcattca cgaggttttg aaatttatca agtttctctt gatcaaaaca
    10021 aagatcgctg gaagcaagct gttgaaaata ttccctggat ttgtgtgaga gatgaaaatg
    10081 gatatcaaac accttatgta ttatcataca acgttacttt tatcccaacc aatttcttga
    10141 tgaataaaaa gggtgaaatt attgctcgtg atttggactt taaacagtta aaccaaatga
    10201 ttgacaacct tttataatat cgtttttttt acaaaaagaa aatgataatc tctcttattt
    10261 tattgaaaaa tgattaaaga ttttattaag gaaacggctg cttcattagg tttcgatgct
    10321 tgtggaattg cacgagcaga agaattgaaa gaagatgctg attttcttaa gggttggtta
    10381 aaagctggtt atcatggcga aatgtattat atggaacaaa attttgagaa aagaatcaat
    10441 cctcaaaaat tagtaccgga ttgtaaaacg gtagtagtaa ctttattaaa ctattatatt
    10501 gattgcccct ctcctactga tgctccccga attgccaaat atgcttatag tgaaactgat
    10561 tatcataaag taataaagca aaagttacga caattggaaa atgaaataaa aaataaattt
    10621 ggtgaaaatt gtttttcaaa agattttcag catgtttttg ttgattccgc tcctattcta
    10681 gaacgacgtt gggctgaaag agcaggttta ggatggttag ggaaaaatac tcaattaatt
    10741 catccgggct taggaagtta tgtgtttatt ggaattctat tgttaaacgt agaggttgag
    10801 ccagatcttc ctatttcgtc tcattgtgga aagtgtaaat tatgttggga agcttgtcca
    10861 acaaaagcat tatctgaaaa tggtttggat gctcgcaaat gtatttctta tcttactgta
    10921 gaatataaaa aagaaatacc accagatttt caaaattgtt tagcaggttt tattgccggt
    10981 tgtgatattt gtgcagaagt ttgtccctgg aataaaaaat gggcagtttc acacaatcat
    11041 gatgaattaa agcccaattc tcatatttta aattggaaat atgaaaattg ggaacatctt
    11101 tctcctgctg attttgaaga aatatttcac
//
LOCUS       DAOSAS010000050        11068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_3932_length_11068_cov_13.332516, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000050 DAOSAS010000000
VERSION     DAOSAS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11068
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3932_length_11068_cov_13.332516"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..889)
                     /gene="tpiA"
                     /locus_tag="PLR72_03615"
     CDS             complement(134..889)
                     /gene="tpiA"
                     /locus_tag="PLR72_03615"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOK36329.1"
                     /translation="MRKNIVAGNWKMNKTLPEGLKLATELNTLLANVKLNCQIIIAPP
                     FIHLASIVAAIDTKKIGVAAQNCADKEWGAYTGEVSAGMVKSTGAEFVILGHSERRTY
                     YGETPEILKTKVELALKYELTPIFCIGEILSEREAGKHFEVVRSQLEKSLFHLPAEEF
                     SKLILAYEPVWAIGTGKNATPEQAQEMHAFIRKTIEERYSSLVADNLSILYGGSCNPG
                     NAKELFANPDVDGGLIGGASLKSQDFKAIIDAF"
     gene            complement(988..2133)
                     /locus_tag="PLR72_03620"
     CDS             complement(988..2133)
                     /locus_tag="PLR72_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOK36330.1"
                     /translation="MTYNSTKKKKSKSHKETKTANFFLELARIILGLVFIFSGFVKAI
                     DPLGSAYKIEDYLIAFGHPFSYFIFLSLPFAILLSTFELTLGFNLILKIYPRITIILT
                     AIFLTALTALTLYLALKNPITDCGCFGDAIILTNWQTFYKNLVLISLAVLLLIYRKRF
                     TPLLLPHIEPFALVIFALLGIVISIYCYHHLPLIDFLPYKVGVNIPEAMHIPEGAKTD
                     QYETTFIYEKNGQRKEFTLENYPKDDSTWVFIDQKTTLKSIGYRPPIHDFVIFDTQDE
                     DITDIILQENNSAYLLIMYDVNKASKVGAKKAEKVYQKAQSTNSFFCAVTASSDEEIQ
                     KFVQATGVTYPFCKADPIMLKTIVRANPGLVLIKNGTIMGKWNWRDF"
     gene            complement(2213..2773)
                     /locus_tag="PLR72_03625"
     CDS             complement(2213..2773)
                     /locus_tag="PLR72_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HOK36331.1"
                     /translation="MDQTEQQFDKIVSICKDIFIKKLKDYGASWRLLRPSSVTDQLFI
                     KANRIRSIQMKGISKINEDIKSEFIGIINYGIIGLIQLELGFAEKVDISTEQAVELYD
                     KYIQKSKDLMSNKNHDYDEAWRSMRVESFVDIILMKICRIKEIENHAGKTLISEGIDA
                     NYYDIINYSIFALIKLEEMEKTHNNH"
     gene            complement(2979..3347)
                     /locus_tag="PLR72_03630"
     CDS             complement(2979..3347)
                     /locus_tag="PLR72_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOK36332.1"
                     /translation="MGKNDFQKKNNAKKDETPEFVDFVVTARKYKTLLTKKYLNRKKW
                     ENPSPYEIYSYIPGTIVEVFVKEGEVVEEGEPLLTLEAMKTLNRIEMPFTAKIKKINV
                     KPGDQIPKDYLIIELEPVEE"
     gene            complement(3454..4998)
                     /locus_tag="PLR72_03635"
     CDS             complement(3454..4998)
                     /locus_tag="PLR72_03635"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOK36333.1"
                     /translation="MTTKKNIIALRQKKAIVQKGGGDKAIEKQLAMGKMTARDRIRAL
                     LDEGSFYEYDLFVQHEEKNFGMDTKDLPGDGVITGTGTINGQPVCVYAQDFTVMGGSL
                     GIQHARKITKIMDHALKMKMPCIGINDSGGARIQEGIGALAGYGEIFYRNTMASGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVENISKMFITGPGVIETVLGEKISQEDLGGARV
                     HAEITGNAHFYALSEKECFAQIKELISLIPHNNEEKAPVVEPKKPTFKKSIENIIPAN
                     PKLPYDVRHIIYALVDNSKFFEVQELWAQNIVVGFARMNGETVGFVANQPLYLAGVLD
                     CDASDKAARFIRFCDAFNIPLITLVDIPGYLPGVDQEHAGVIRHGAKVLYAYSEATVP
                     KMTVILRKAYGGGYIAMNSRHLGADFTFAWPDAEIAVMGPEGAANIIFKKEIMEAPDP
                     DAMRQEKVKEYIEKFANPFVAAGKGYIDAVIEPLETRDLLLHALSVSKKKFDYRPAKK
                     HGNIPL"
     gene            complement(5201..5491)
                     /locus_tag="PLR72_03640"
     CDS             complement(5201..5491)
                     /locus_tag="PLR72_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOK36334.1"
                     /translation="MRRSNTESLAEIIHHLLKAQHLEQPLFEKRIINAWPVVLGEQIT
                     QYTSRLEIKKSVLYVNINSSVLRQELFFSREEIKNALNKYVGSEVIKEIVFR"
     gene            complement(5494..6396)
                     /locus_tag="PLR72_03645"
     CDS             complement(5494..6396)
                     /locus_tag="PLR72_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOK36335.1"
                     /translation="MKKIKWLLSFILFCGFANSQVQNKAYLTYIEKYHSLAEKQQKLH
                     NIPASIILAQGLLESSAGQSYLSQRANNHFGIKCNNDWTGETIYWDDDEKNECFRRYK
                     SVSESYEDHSLFLKKRQRYAFLFQLDPTDYQGWAFGLKNAGYATDPAYAYKLISIIEN
                     YNLQRFDKAEFKEATIATSDIYRLSAYISHKVFKNNGVPYVVATPCDTYESIAEEFSL
                     EEERLRNYNEVDAEAELIAWEKVYIRPKKNKASKNHPIHVVQEGESMHSIAQEYGIKT
                     EKLYKLNHLPYTEGARVGQVLKLR"
     gene            6620..7108
                     /gene="cdd"
                     /locus_tag="PLR72_03650"
     CDS             6620..7108
                     /gene="cdd"
                     /locus_tag="PLR72_03650"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOK36336.1"
                     /translation="MYKNNIVIPVTICQFEELSPSYQHLIIKAKEEVKKAYAPYSHFY
                     VGVALELGNGEIITGNNQENSAYPSGLCAERVAIFYANAKYPEVPVKTLAIAAFSNNE
                     FLKKPITPCGACRQVLLETELRFQNDITILLYGSEEIYLIKNVKDLLPLSFDKYQLKG
                     NK"
     gene            7210..7596
                     /locus_tag="PLR72_03655"
     CDS             7210..7596
                     /locus_tag="PLR72_03655"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOK36337.1"
                     /translation="MAKNMLQLEYPLKSASISLLWNKIGTPLGLSEWFANEVIVNDNK
                     YLFKWDNYEETAYLLDIKPLMKIRFQWEDEIENNTYFELKIETNEITGEVALVITDFT
                     DNIEEEDVILLWDKQIDELKRKIGMG"
     gene            7784..9250
                     /locus_tag="PLR72_03660"
     CDS             7784..9250
                     /locus_tag="PLR72_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOK36338.1"
                     /translation="MFRVKIIHKYIFKNFITLFIMTFFICIFILLMQFLWKHVEDLVG
                     KGISISVLFEFFNYSVLTLVPMALPLAILLASLMTFGNLGETSELTAMKASGISLFRI
                     MQPLIFFIALVCVGAFYFSNYVLPISQTKLWTLIFSLKQKSPELEIPVGEFYTNIDGI
                     NIYVREKQGKALKNLMIYDFSAGFNNATVMIADSGEINLTEDKKYLLLSLYNGESFEN
                     LKNQRVTDNPASIPYRRETFRTKEILIDYDAQFNRLDESLLRDQHISKNISQLSYSID
                     SVSKVVKNISIQQSNELLNYSSFARERNTVSASVPIEKNKNITLNADSLFLSLNQSSM
                     IKAVEYAISQAQQIKDQVGFNKIILNEPIMYVRRHQVEWHRKFTLSFACLIFFFIGAP
                     LGAIIRKGGLGMPVVISVVMFIIYYIIDNTGYKMAREGLWSTFAGMWLSSFVLLPVGI
                     FLTYKAVKDAPLLDAEEYTKIWNKMNQKLNSWIDKIKI"
     gene            9282..10532
                     /locus_tag="PLR72_03665"
     CDS             9282..10532
                     /locus_tag="PLR72_03665"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOK36339.1"
                     /translation="MDTKLNTIEEAIDDIRRGKFVIVVDDEDRENEGDFICAAEKITP
                     EMVNFMITHGRGVVCAPLTEERCKELDLEMQVDINTSIHSTPFTVSVDKLEGCTTGVS
                     AADRAATLRALADPASKPETFGRPGHIFPLRAKSKGVLRRAGHTEAAIDLIRLAGLYP
                     VGALIEIMNEDGTMARLPQLIEIAKRFDLKIVTIEDLIAYRLKTESLIEKGEKVDLPT
                     LYGHFNLIPFRQISNGKEHIALIKGEWQKEEPILVRVHSSCVTGDIFGSLRCECGEQL
                     HKAMQMIEKEGKGVIVYMNQEGRGIGLMNKIKAYRLQEQGLDTVDANLHLGFDADERD
                     YGVGAQILKSIGVTKIKLLTNNPVKRIGLEAYGLSIVENVPIEIQPNPYNAFYLEIKK
                     KRMGHDLKFIKDESDPNNEKPTNV"
     gene            10844..>11068
                     /locus_tag="PLR72_03670"
     CDS             10844..>11068
                     /locus_tag="PLR72_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36340.1"
                     /translation="MSEIIKNAVLAGIGLISLTREKAEEFAKELIKKGELSENEKAKF
                     VKDVLEQSEKSKREIEEKIEKTVETVMAKMN"
BASE COUNT         3738 a         1761 c         1723 g         3846 t
ORIGIN      
        1 taatcaacat ttttttattt ttttctcttt aagaaaattt cttttctttt aatatcttca
       61 aagatataaa aataaaaaat cccataagca atacttacag gatttttatc aaaatatttt
      121 caaaaacagt gatttaaaaa gcatcaatga tagctttaaa atcttgcgat ttcaatgaag
      181 ccccaccaat aagtccgcca tcgacatcag gatttgcaaa cagttctttt gcatttcctg
      241 gattacaact cccaccataa agaatagaaa gattatcagc caccagagaa gaatatcttt
      301 cttcaatggt ttttcgaata aaagcatgca tttcctgagc ttgttcggga gtagcatttt
      361 taccagtgcc aatagcccaa actggttcat aagcaagaat cagttttgaa aactcttctg
      421 ctggaagatg aaacaacgat ttttccaatt gtgatctgac tacttcaaaa tgttttcctg
      481 cttctctttc gctcaatatt tcaccaatac aaaaaatagg cgttaattca tatttcagtg
      541 ccaattctac tttggttttc agaatttcag gtgtttcccc gtaataagtg cggcgttctg
      601 agtgtcctaa aatgacaaat tctgctccag ttgattttac cattccagca cttacttcgc
      661 ctgtataagc tccccattcc ttatcagcac aattttgagc agctactcct attttttttg
      721 tatcaatggc agcaacaata cttgctaaat gaatgaacgg aggagcaata attatctgac
      781 aatttaattt aacatttgcc agaagcgtat tcaattcagt tgctaatttc aacccttctg
      841 gaagggtttt attcattttc caatttcctg cgacaatgtt ttttctcatc tttttatatt
      901 ttatgataaa ttatttataa aataaatttg ataatcaatg atataggtca aatttaatta
      961 attcttcacc aatgaataaa tttcaatcta aaaatctcgc cagttccatt ttcccataat
     1021 tgttccattt tttattaaaa ccaaccctgg attggcgcga acaattgttt tcaacattat
     1081 gggatcagcc ttacaaaaag gataagttac gcccgtagct tggacaaatt tttgaatctc
     1141 ttcatcagaa gaagctgtaa cagcacagaa aaagctgttt gttgactgtg ctttttgata
     1201 aactttttct gcttttttgg caccaacttt tgaagctttg tttacatcgt acataattaa
     1261 taaataagca gaattatttt cttgaagaat tatatcagta atatcttcat cttgagtatc
     1321 aaaaataaca aaatcatgaa taggaggacg ataacctatt gattttaaag tggttttttg
     1381 atcgataaat acccacgtag aatcatcttt tggataattt tcgagcgtaa attcttttcg
     1441 ttgcccattt ttttcataaa taaaagtcgt ttcatattgg tctgttttgg caccttcagg
     1501 aatatgcata gcttctggaa tatttacacc tactttatat ggtagaaaat cgattaatgg
     1561 taaatgatga taacaataaa ttgaaataac tattcccaac aaggcaaaaa ttaccaatgc
     1621 aaatggttca atatgaggta aaagcagagg agtgaatctt tttcggtaaa ttaaaagcaa
     1681 tacagctaag gagataagta ccagattttt gtaaaaagtt tgccaattgg tcaatataat
     1741 tgcatcacca aaacaaccac aatcagttat cggattttta agtgccagat aaagtgtgag
     1801 ggcagtaagt gctgtcaaaa aaatagcggt taaaatgata gtaatacgtg gataaatctt
     1861 taatatcaag ttaaatccaa gtgtcaactc aaaagtagaa agtaaaatag caaaaggaag
     1921 agaaagaaaa atgaaataag aaaaagggtg accaaaagct attaaataat cttcaatttt
     1981 ataggctgaa cctaagggat caattgcttt aacaaagcct gaaaaaataa aaaccaagcc
     2041 caaaattatt cgagccaatt caagaaaaaa attagccgtt tttgtttctt tatgagattt
     2101 tgattttttc tttttcgtgg aattgtaagt cattttctat aaaataaact taaaaaacaa
     2161 aagatttttt attttttcaa ctgtgattct tattaaaatg taattcaatt atttaatggt
     2221 tattgtgcgt tttttccatt tcttccagtt ttatcaaagc aaaaattgaa taattgataa
     2281 tatcataata gttggcatcg attccttctg aaattaaagt ttttcctgca tgattttcaa
     2341 tttcttttat acgacagatt ttcataagaa taatatccac aaatgactcc actcgcatac
     2401 ttcgccacgc ttcatcataa tcatgatttt tattggacat caaatctttg cttttctgga
     2461 tatatttatc gtatagttca acagcttgtt cagtacttat atctactttt tcggcaaagc
     2521 ctaattctag ttgaattaaa ccgatgattc cataattgat gattccaata aattcagatt
     2581 ttatatcttc gttgattttt gaaattcctt tcatttgaat agacctaata cgattggctt
     2641 tgataaaaag ctggtcagta actgaagaag gacgtaaaag gcgccaggaa gcaccgtaat
     2701 cttttaattt tttaatgaaa atatctttgc aaatagaaac aattttgtca aattgctgtt
     2761 cagtttgatc cataagtaaa aaattaagaa tctatttata aaaacaaatt acatttttat
     2821 ttaatctctt tctcattgaa tttttggtca tttgcaaaag tacaactttt aatcaaaaaa
     2881 gacaattaac acttaaatcc tcatttaaaa aaatctcttt gtctttgaaa atttaaaaat
     2941 catacaaaga gatgaattat ctgaatattt tttaaaaatt attcttcaac gggttctaac
     3001 tcaatgatta aataatcctt aggaatttga tcgccaggtt taacattaat ttttttaatt
     3061 ttagctgtaa aaggcatttc gatacgattt agtgttttca tcgcctctaa tgttaaaagt
     3121 ggttctcctt cttctactac ctcgccttct tttacaaaaa cctccacaat agtacctgga
     3181 atatatgagt aaatttcata aggactagga ttttcccatt tttttctatt aagatatttt
     3241 ttagttaaaa gtgttttata ttttcttgct gtaactacaa agtcaacaaa ttcaggggtt
     3301 tcatcttttt ttgcattatt ttttttctga aaatcatttt ttcccattta ttcctaattt
     3361 ttttattttt aaaattaaat aatcgtttct gttttttgtt aactttctaa taaaagtctg
     3421 aagcagaagt gaaattcaat ataaaatatt gaattataaa ggaatatttc catgtttttt
     3481 tgcaggacga taatcaaact ttttctttga tacactcaga gcgtgaagca ataaatcgcg
     3541 cgtttcaagc ggttcaatta cggcatcaat ataacctttt cctgctgcca cgaaaggatt
     3601 tgcaaatttt tcaatgtatt ctttcacttt ttcttgtcgc atagcatcag gatcaggagc
     3661 ttccataatt tcttttttga aaatgatatt ggctgctcct tctggtccca tcacagcaat
     3721 ttcggcatct ggccaggcaa aagtaaagtc agctcctaaa tgcctggaat tcattgcaat
     3781 ataaccaccg ccataagctt ttcttaaaat aactgtcatt tttggtactg ttgcttcgga
     3841 ataagcatat aaaacttttg ctccgtgacg aatgacaccg gcatgttcct gatctacacc
     3901 aggcaaatag ccaggaatat ccaccagagt aattaatggg atattaaagg catcacaaaa
     3961 acgaataaat cgagcagctt tatctgaagc atcgcaatcc aatacccctg ccaaatacaa
     4021 aggttgattg gctacaaaac caaccgtttc tccattcatt cgcgcaaaac caacaacaat
     4081 attttgagcc catagttcct gcacttcaaa aaatttagag ttatccacca gtgcataaat
     4141 aatgtgtctt acgtcataag gtaatttagg gttggcagga attatatttt caatactttt
     4201 tttgaaagtt ggttttttag gttctactac aggagctttt tcttcattat tatgaggtat
     4261 taaagagatt aattctttaa tttgtgcaaa gcattctttc tcactcaacg cataaaaatg
     4321 agcatttccg gtaatttctg catgtacccg tgctcccccc agatcttcct gtgaaatctt
     4381 ttcacccaag acggtttcaa ttactcctgg accggtaata aacattttag aaatattttc
     4441 aaccacaaaa acaaaatcag tcaacgcggg tgaatatact gctccgccgg cgcacggacc
     4501 taaaattaca gaaatttgag gaattacccc tgatgccatt gtattacgat aaaaaatttc
     4561 gccataacct gctaaagctc caataccttc ctgaatacga gcaccaccag agtcgttaat
     4621 gccaatacaa ggcattttca tctttaaggc atgatccata attttagtta ttttacgagc
     4681 atgttgtatt cccaatgaac ctcccatcac cgtaaagtct tgagcataaa cacatacagg
     4741 ttgaccatta attgtacctg ttcctgtaat tactccatcg cccggtaaat ctttggtatc
     4801 cattccaaaa ttcttttctt cgtgttgaac aaaaaggtca tattcgtaaa acgaaccttc
     4861 atctaacaac gcacgaatac gatcgcgtgc tgtcattttt cccatcgcca attgtttctc
     4921 gatggcttta tctccccctc ctttttgaac aattgctttt ttttgtcgca aagcaataat
     4981 attctttttg gtagtcatga taaggtttta taaattaatt aaacataaaa aattatcttc
     5041 tcatacccaa aatatgggtt ccttttcaaa aagcaaaagt actacttttt tatttatttc
     5101 ttttgcttaa tcaatccatt tctctatttc ttgaggataa aatgcttatt tataaatagt
     5161 tataaatatt ttatattgct ttaaaaaacg aacttcattt ttaacgaaaa acaatctctt
     5221 tgataacttc agaccctaca tatttattta aagcattttt aatttcttcg cgggaaaaaa
     5281 acaattcttg tcgcaaaaca gaagagttaa tatttacata aaggacagat ttttttattt
     5341 ctaaacgaga ggtgtattga gtaatttgtt cacctaaaac aacaggccag gcattaataa
     5401 ttcttttttc aaataaaggt tgctctaagt gctgtgcttt gagcagatga tgaataattt
     5461 cagcaagtga ctctgtatta cttcgacgca tatttatctt aattttaaaa cctgacccac
     5521 tcgtgcccct tctgtataag gtaagtgatt gagtttataa agtttttctg tttttattcc
     5581 atattcttga gctatgctat gcatggattc accttcttgt actacgtgaa tgggatgatt
     5641 ttttgaagct ttgtttttct ttggacgaat ataaactttt tcccatgcta ttaattcagc
     5701 ttcagcatct acttcgttat aatttcgcaa tcgttcctct tccagactaa attcttctgc
     5761 aatactttca taagtatcgc atggagtagc aactacatac ggaacaccat tgtttttaaa
     5821 aactttatga gaaatataag cgcttaagcg gtaaatatca gatgttgcaa ttgttgcttc
     5881 tttaaattca gccttatcaa atctttgcaa attataattt tcaatgattg aaatcaactt
     5941 gtaagcatag gcagggtcgg tagcataacc tgcattttta agtccgaatg cccatccttg
     6001 ataatcagta ggatctaatt gaaacaaaaa agcataacgt tgcctttttt tcagaaaaag
     6061 agaatgatcc tcgtacgatt ctgatacgct tttatatctt ctgaaacatt catttttctc
     6121 atcatcgtcc caataaattg tttcgcctgt ccaatcattg ttacacttta ttccaaaatg
     6181 attgttggct cgttgagaga gataactttg ccctgctgac gattctaata atccttgtgc
     6241 taatatgatg ctggcaggaa tgttatgtaa tttttgctgt ttttctgcca gagaatgata
     6301 tttttcaatg taagttagat aagccttgtt ttgcacttga ctattggcaa agccacaaaa
     6361 taaaataaaa gataataacc actttatttt tttcattata aaaacaatta gttagcaaat
     6421 atcgatattc aaatttgtta ttttaaatta tgataaagtg tctcttaaca aaatgtaaag
     6481 atatatacaa aaataattta tttttccgaa aataagccct aaaaaagaat taatttccta
     6541 tttttgtaat ttggcattaa attttaatat taatgtcatt tgattttcaa taagttaaaa
     6601 acattaaagg aaattatgta tgtataaaaa caatattgta ataccggtca ctatttgtca
     6661 atttgaagaa ttgtcccctt catatcagca tctcatcata aaagcaaaag aagaggtaaa
     6721 aaaagcctat gctccttatt cacattttta tgtaggagta gctttagaac tggggaatgg
     6781 cgaaattatc acaggaaata atcaagaaaa ttcggcttat ccttcaggtt tatgtgccga
     6841 acgagttgca attttttatg caaatgcaaa atatcctgag gtacccgtta aaacattagc
     6901 aattgcagct tttagcaaca atgaattctt aaaaaaacca atcactcctt gtggagcttg
     6961 ccgacaagtt ttgcttgaaa ctgagcttcg ttttcaaaat gatattacta ttttgcttta
     7021 tggaagcgaa gaaatttatt taattaaaaa cgtaaaagat ttacttcctt taagttttga
     7081 taaatatcaa ttaaaaggca ataaataatc gcttcttttt ctttttcctt gtttatttga
     7141 attattattt ttatattggc tctcgtttac taacagtatt tttttcttgt gaattaaaat
     7201 taagagaaga tggcgaaaaa tatgcttcaa ttggaatatc cacttaaatc agcttctata
     7261 agtttgcttt ggaacaaaat aggtactccg ctgggacttt ctgaatggtt tgccaatgaa
     7321 gtaattgtaa atgataataa atatcttttt aagtgggata attatgaaga aacagcttat
     7381 ttgttagata ttaaaccttt gatgaaaatt cgttttcaat gggaagatga aatagaaaat
     7441 aatacctatt tcgaactaaa aattgaaacc aatgaaataa ccggagaagt tgcgctagta
     7501 ataactgatt tcactgataa tattgaagaa gaggatgtta tattgctttg ggataagcaa
     7561 atagatgaat tgaaacgtaa aataggtatg gggtaataaa attgtttttt aaaattattt
     7621 ttacttcaat taatttaatt accatttgtt ttggagaact tttccactta acaaatggtt
     7681 ttctatttat aaataaaatt atagctttca atgaatgtta atttcatatt gtttctccta
     7741 aattattgaa ctttttggtg taattttgca ttttaaaaaa atcatgttca gagtaaaaat
     7801 aatacataaa tatattttca aaaattttat cacgctcttt atcatgactt tctttatctg
     7861 catctttatt ttgttgatgc agtttttatg gaaacatgta gaagatttgg tgggtaaagg
     7921 aatcagcatt tctgttttat ttgaattttt caattattca gttctaactt tggtgccgat
     7981 ggctttaccg cttgccatct tgctcgcttc actaatgact tttggaaatc tgggagaaac
     8041 ctctgagctt acagctatga aagcatctgg aatttctctt tttagaatca tgcagcctct
     8101 tattttcttt atcgcccttg tttgtgttgg tgctttttat ttctcaaatt atgttttgcc
     8161 tatctctcaa acaaaattat ggacattaat tttttcattg aaacagaaat cacctgaatt
     8221 ggaaatacca gtaggagaat tctatacaaa catagatggc ataaatatct atgtacgtga
     8281 aaaacaagga aaagctctaa aaaatttaat gatttatgat ttttcggctg gatttaataa
     8341 tgcaacagta atgattgcag attctggcga aataaattta acagaagata aaaagtattt
     8401 attgctctct ctgtacaatg gagaaagttt tgaaaatctt aaaaatcaac gtgtcactga
     8461 taatcctgca agtattcctt acagacgaga aacttttagg acgaaagaaa ttttaattga
     8521 ttatgatgca caatttaatc gtttagatga atcactactt cgcgatcaac atatcagtaa
     8581 aaacatttca caactttcgt attccattga ttctgttagt aaagttgtaa aaaatataag
     8641 tattcagcaa tcaaatgaat tattaaatta tagttcattt gctcgtgaaa gaaacactgt
     8701 ttcagcatca gttccaatag aaaaaaacaa aaacatcact ttaaatgccg attcactttt
     8761 tttgtcgttg aatcagtctt ctatgataaa agctgtggaa tacgccattt cacaagctca
     8821 gcaaataaag gatcaagttg gattcaataa aattatatta aatgaaccta tcatgtatgt
     8881 tagaaggcat caggtagaat ggcatcgaaa atttacgctt tcttttgcat gcttaatatt
     8941 tttcttcatt ggagcacctc ttggagctat tattcgtaaa ggtggtttgg gaatgcctgt
     9001 ggtgatttcg gtagtaatgt ttattattta ttatatcatt gataatacag gctataaaat
     9061 ggcacgggaa ggtctatggt caacttttgc gggcatgtgg cttagctctt ttgtactttt
     9121 accagtaggt atatttttaa cttataaagc agtaaaagat gctccactcc tggatgctga
     9181 agaatataca aaaatatgga ataaaatgaa tcaaaaactg aattcatgga tagacaaaat
     9241 taaaatataa gatttgatat tcgattaaat gataattatt tatggacaca aaattaaaca
     9301 ccattgagga agcaattgat gatatccgcc gcggaaaatt tgttattgta gttgatgatg
     9361 aagatcgtga aaacgaagga gattttattt gtgctgctga aaaaattact cctgaaatgg
     9421 tgaattttat gattacacac ggaagaggag tagtgtgtgc tcctttgacg gaagaacgat
     9481 gcaaggaatt agatttggaa atgcaagttg atattaatac ttccatacat tccactcctt
     9541 ttacggtaag cgtagataaa ttagaaggtt gtactactgg cgtttctgct gccgatagag
     9601 cagctacttt aagagcattg gcagatcctg catccaagcc tgaaactttt ggacgtcccg
     9661 gacatatttt tcctttacga gcaaaatcga aaggagtttt acgaagagct ggtcatactg
     9721 aagcagcaat agatctaatt cgcttagcag gtttatatcc agtaggtgca cttattgaaa
     9781 taatgaatga agatggcaca atggcacggt tgcctcaatt aatagaaatt gctaaacgtt
     9841 ttgatttaaa aatagtgact attgaagatt tgattgctta tcgactcaaa actgaatcat
     9901 tgattgaaaa aggagaaaag gtcgatttgc ctacccttta tggacatttt aatttgatcc
     9961 cattccgtca aatttcaaat ggaaaagaac atatagcttt aataaaaggt gaatggcaga
    10021 aagaggaacc tattctggtg cgagtacatt catcttgcgt aaccggggat atttttggtt
    10081 ctctacgttg tgaatgtggt gaacaattac ataaagcaat gcaaatgatt gagaaagaag
    10141 gaaagggagt gattgtttat atgaatcagg agggaagagg gattggcttg atgaataaaa
    10201 ttaaagctta tagattgcag gaacaaggac ttgatacagt tgatgctaat ttacatttag
    10261 gttttgatgc tgatgaaaga gattatggtg ttggagcaca aatattaaaa tcaatcggcg
    10321 taacaaaaat caaactttta accaataacc ctgtaaaacg cataggttta gaagcttatg
    10381 gattgtcaat tgttgaaaat gttcctatcg aaatccaacc taatccttat aatgctttct
    10441 atttggaaat taaaaagaaa cgaatgggac atgatttaaa attcataaaa gatgaatctg
    10501 atccaaataa tgaaaagcca accaatgtat gagaatatag tttctccttt tttgagattg
    10561 gctattgaat ttatggttga ttataagatg aaagtgacca attaataggc ataagttagt
    10621 gctctctttt tgcatgaaat aataattttc cacaatcttt tctcttgttt ctaatgtttc
    10681 taagaattta aaaagaaaaa taagttgttt ctaaacaata gtgtttttaa aaaaatgttg
    10741 tatttttaca taaaattcag agtaaaaaaa tatttatcat tttttagttt atcaaagaaa
    10801 aatttttaac cacttatctc ataaaataaa ggaggatcgc attatgtcag aaattatcaa
    10861 aaatgcagtt ttagcaggca tagggcttat ttcattgacc cgcgaaaaag ctgaagaatt
    10921 tgccaaagaa ctgatcaaga aaggcgagtt gtctgaaaat gaaaaggcta aatttgttaa
    10981 agatgttctg gagcaatcag aaaaaagcaa aagagaaata gaagagaaaa tagaaaaaac
    11041 agttgaaacg gtaatggcaa aaatgaat
//
LOCUS       DAOSAS010000051        10803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4036_length_10803_cov_11.781355, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000051 DAOSAS010000000
VERSION     DAOSAS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4036_length_10803_cov_11.781355"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLR72_03675"
     CDS             complement(<1..449)
                     /locus_tag="PLR72_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOK36341.1"
                     /translation="MVKKSVLGRGLDALIDTENIYTSGSSSINEIDIDLILPNPNQPR
                     SYFDEDSLSELAASIRELGVISPITLKKNDDGTYLMIAGERRYRAAKMIGLKTIPAYI
                     KTAADEEVVEMALIENIQREDLNAIEIALAFYRLIEEYQLTQEQLSER"
     gene            complement(500..1273)
                     /locus_tag="PLR72_03680"
     CDS             complement(500..1273)
                     /locus_tag="PLR72_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK36342.1"
                     /translation="MGKIISLANQKGGVGKTTTAINLAASLATLGEKVLLVDADPQAN
                     ASSGLGINVQSLQFTIYECLIDGVEPKDAILPTEIENLFILPSHIDLVGAEIEMLNLD
                     HREKVMQNLLRPLKPYYNYILIDCSPSLGLITVNSLTASDSVIIPVQCEYFALEGISK
                     LLNTIKIIKSKLNPSLEIEGFLLTMYDNRLRLANQVYAEVKKHFQEMVFDTVIARNVR
                     LSEAPSYGKPVMVYDPESKGAINYQDLAKELIAKQKVKL"
     assembly_gap    1316..1415
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1416..2930
                     /gene="gldG"
                     /locus_tag="PLR72_03685"
     CDS             <1416..2930
                     /gene="gldG"
                     /locus_tag="PLR72_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOK36343.1"
                     /translation="TLYLEGDLNPGFLRMKKSTIQMLEELSAYSKKGIEIKTINPSLA
                     KSSTEREKNYLKLSSHGLTPTAVYERDKEGKAIQKIIFPWLEIEYKGNKTLVNLLKNL
                     PGNSGEQNINISIENLEFEITDAIRRLTSTQVNKIAFLEGHGELSEAETYDISKALSR
                     YFQIDRGIIGTDASILDDYKVLIVAKPMFPFSEKDKFIIDQYIMKGGRILWLIDGVQL
                     SKENLSTVGFSPAWALDINLNDQLFRYGVRINPVLLQDVQCVLVPVNVAPQDQSPHFE
                     PMPWYFAPLLLTSYQHPITKNITEIKTDFCSSIDVVGDNPHTKSTVLLATSNNTHIIP
                     TPSTIDLRILPKANDKEYFNLGYVPVAILVEGSFESVFRNRMLPAGLININKVQYQSK
                     PTRQIFVADGDIIRNETSGLATDSTTLSLGFDRYMNQQFGNKDFIQNAVLYLTDDEGW
                     MQLRTKTFKLRLLNKNAVNDKKNICQVINISVPIILLALFGIIYQWIRKRKYSK"
     gene            3347..4030
                     /locus_tag="PLR72_03690"
     CDS             3347..4030
                     /locus_tag="PLR72_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOK36344.1"
                     /translation="MKDIRESILLEASFLFHQLGIKNISIDEICSNLRISKKTFYSYF
                     DQKQDLVDAVLFYQEEFYLKKLEGILKNKNAIEFLILSLKEIKKRANCEPTLMWNDIK
                     KYYPEVFKKHILIRQDFLKKLFETNLKRGVEEGFYRKDLDIILISILYSEHLKNIFNT
                     LNKYSKKYSKKRLIDFFIDVIIHVIANEEGLQYVQENYKGVEENPVEMKKNENILNNY
                     RSESKGVFN"
     gene            4067..5404
                     /locus_tag="PLR72_03695"
     CDS             4067..5404
                     /locus_tag="PLR72_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOK36345.1"
                     /translation="MNKKHLKRIFFVVISLLLINSKLYAQDTLIVTLSDAIEIALSES
                     PTIKIADKEIERVDYSKKEKRGALFPSINAVGNYSRAIKKQKLFFSLPGMPPRPEGVE
                     VGQDNTFSGGISASLPIIAPTLWATIQMSELDAELALESARSSKLSLINEVSKSYYAT
                     LLAQDSYDVFKKSYDIAIENAKVIENKYKQGVVSEFEWIRADVQVKSASTNLVSAENA
                     VTLSILKLKMLMGIDMFTGLKVKGNLADYEATKYSDVLAIDTTTLQANSDLKQFDIKT
                     QQLQQTLKIQKSTLMPTLSASFNYQYMTMANDSVAFSNYHWFPTSTGAITLAIPLFQG
                     GEKINKQKQIKVQLDEMKYQRDNLRRGLELQAMSYLDNINQAIKTIETNKEGLRQAEK
                     ALLISQKMYEVGASTYLDLSNAELVYTQAGLAYNQSIYNYLSAKADLEKLLGK"
     gene            5456..6475
                     /locus_tag="PLR72_03700"
     CDS             5456..6475
                     /locus_tag="PLR72_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOK36346.1"
                     /translation="MKRTIEFGILFLAIVFGFTSCGKSGQKKTKIESEIPNVRIQQVS
                     LRDVERYANLTATILPEVKNNVAPAAPGRIRKIFVEVGDHVSKGQKLVQMDDANLSNL
                     ENQVANYQRIYNRVLELFNVGGASQQELDNAKLQLDVATTNLQNLAENTYLLSPTNGV
                     VTARNYDNGDIYSAQFPVLVVMQINPVRAIVNVSETNYSDVKLGMPAQVTFDILKNEF
                     FTGKVNLIYPTIDERTRTFSVEIKLNNNDYKIRPGMFARVKLNFGTVKSIVVPDQAVI
                     KQSGSGEYFVYIYKDGKVTYQKVELGQKIGNEYEIISGIEDGAQVVVAGQARLADGVK
                     VNVVK"
     gene            6538..>9242
                     /locus_tag="PLR72_03705"
     CDS             6538..>9242
                     /locus_tag="PLR72_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOK36347.1"
                     /translation="MSLYESSVKRPIMTSLIFIGVVILGLFSLSKLPVDLLPNVEPNI
                     LMVVTNYSGASALDIETNVTRPLENVLNTVENLKNITSNSKENTSIIIMEFEFGQDLD
                     VVSNDVRDKLELVKPYLPEGTTNPVIFKFSTDMMPIVIISATADKSIPALYKILDDNV
                     ANPLARIKGVGAVSISGAPQREIQVYIDPVKLEAYHLTIEAVGQKIRLENINTPSGSM
                     DIGNKTYSLRVQGEFKDPSELRKVVVGTYGNKTIYLSDIAIISDSHEERVQESYTNGV
                     QGATIKIQKQTGANTVAIVNAIREKLPEIQKTLPSDIKLNVIVDSSDNIKKTIAGLEE
                     TIMYAFIFVLLVVLFFLGRWRATIIIILTIPISLIAAFIYLAATGGTLNIISLSSLSI
                     AIGMVVDDAIVVLENITKHIEKGSRPTSAAIHGTDEVATAVVASTLTIVAVFFPFTLV
                     SGLAGILFDQLGWMVTLIMIVSLVCAMALTPMLSSQLLTVEKGHGKFFTKVYSPIERF
                     LNNLDNWYAGLINWAVRHRWQMVLIAAGFFILSLIPLIGIGTEFIPPQDNARISATVQ
                     LPIGTRMEISKETAEELTKLWREKYPEIETINYTVGPASSDNVLGWVMTTGTHLISYT
                     FQLVDANERERSIFEIGDSIRHDLNIRPEIAKSTVTAGGAMGRIGGQSIIDVEIYGYD
                     FNQTDKVAKELTNRIDKNIKGLVNTTISREDYSPEYQVIFDREKLALNGLDIATASTF
                     LRNRINGLVASLYREEGDEYNIRVVYAPEYRETIEDIENILIYNNQGQAIRLKDLGNV
                     VETLTPPYIQRKNRERVITVSSTVSGTTIDKAVRGLRTQLSQMDIPTGIDAEITGTYE
                     DQQESFADLGTLLIIVILLVYIVMASQFESLTYPFIIMFALPFAFS"
     assembly_gap    9243..9342
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9410..9706
                     /locus_tag="PLR72_03710"
     CDS             9410..9706
                     /locus_tag="PLR72_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36348.1"
                     /translation="MKSIFIVFDQGFYHQVLEILYINHHVRGFTSWERVLGRGSKTGE
                     PHYGTHAWPSLNGAILTVVPDEKVKPILEDLKALDATSDVMGIRAFVWNIEDGM"
     gene            complement(9795..10748)
                     /gene="tal"
                     /locus_tag="PLR72_03715"
     CDS             complement(9795..10748)
                     /gene="tal"
                     /locus_tag="PLR72_03715"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOK36349.1"
                     /translation="MNLLEQLKTYTKVVADTGDFESIINYKPLDATTNPSLIFAASQQ
                     EKYQHLILDAIQYAKKSHIDKSQQLKLALDKLSVNFGIKILEIVPGRVSTEVDARLSF
                     DTGGTIERARDLIALYEKEGISRERILIKIASTWEGIEAAKILEKEGIHCNLTLLFSL
                     IQAIACAEARVTLISPFVGRILDWYKKEGFSFNSPSEDPGVKSVVSIYNYYKKFDYPT
                     QIMGASFRNIQQIIELAGCDLLTISPTLLKELEQTEGILERKLDPEKAKSNDVQRITI
                     DEKIFRWMLNEDAMATEKLAEGIRNFTKDLRKLENKIAELL"
BASE COUNT         3617 a         1550 c         1854 g         3582 t
ORIGIN      
        1 cgttcactta attgttcctg agtaagttga tactcttcta tcaagcgata aaaagccagt
       61 gcaatttcga tggcattcaa gtcttcccgt tggatgtttt ctatcagagc catttccact
      121 acttcttcat cggcagccgt tttaatatac gcaggaatag ttttcagtcc tatcatcttt
      181 gctgccctat atcgtcgttc tcctgctatc attaagtaag taccgtcatc gttttttttc
      241 aatgtgatgg gtgaaataac acctaattca cgaatggaag ctgctaactc tgaaagcgaa
      301 tcttcgtcaa agtaagaacg tggttgattg ggattaggta aaatcaaatc aatatcgatt
      361 tcgtttatcg acgatgaacc gcttgtataa atattttcag tgtcaattaa ggcatccaga
      421 cctcttccta atactgactt ttttaccata acttttttct gtggaacgaa ttttttattt
      481 ctttattatt ttaaactgat taaagtttta ctttttgctt ggcaatcaat tctttagcta
      541 aatcttgata attaatagct ccttttgatt ctggatcata taccatgact ggcttaccgt
      601 aactgggggc ttcacttaaa cgaacattgc gagcaataac ggtatcaaaa accatttcct
      661 ggaaatgttt ttttacttca gcatatactt gattggctaa acgtagtctg ttatcataca
      721 tcgtaagtaa aaaaccttca atttccagag aaggattcaa tttgcttttt atgattttaa
      781 tggtatttaa aagtttacta attccttcaa gggcaaaata ttcacattga acaggaatga
      841 ttacagaatc agaagcagta agtgaattta ctgtgataag ccctaaagaa ggagaacaat
      901 caatcaaaat ataattgtaa taaggtttta aaggtcgaag caaattttgc attacttttt
      961 ctcgatggtc taaattcaac atttcaattt cagccccaac taaatcaata tgagaaggaa
     1021 gaataaacag attttcaatt tctgttggca aaatggcatc tttaggttca actccatcta
     1081 ttaagcattc ataaatggta aattgcaatg attgaacatt gatacctaac cctgaagaag
     1141 catttgcttg cggatcagca tctactaata aaactttttc accaagggtt gctaatgaag
     1201 cagctaaatt aatagctgtt gttgttttac caactcctcc tttttgattg gctaatgata
     1261 ttatttttcc cataaattat taataatcaa tttatcaata acttaataaa attgannnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnactct ttatcttgaa ggagatttaa
     1441 atcctggttt cttgaggatg aaaaaatcaa cgattcaaat gctggaggaa ttatctgcct
     1501 attcaaaaaa aggaatagaa ataaagacta taaatccttc actggcaaaa agttcaacag
     1561 aaagagaaaa aaattattta aaattgagtt cgcatggctt aactccaaca gcagtttatg
     1621 agcgtgataa agaaggaaaa gccattcaaa aaattatttt tccctggctt gaaattgaat
     1681 ataaggggaa taaaacattg gtaaatttat tgaagaattt accaggcaat tctggtgagc
     1741 agaacatcaa catttccata gaaaaccttg aatttgaaat tacagatgct attcgccgat
     1801 tgacaagtac ccaagtaaat aaaattgctt ttctcgaagg tcatggcgaa ctttctgaag
     1861 cagaaactta tgatatcagt aaagctttaa gccgatattt tcaaattgat cgaggtataa
     1921 ttggcacaga tgcttcgata ttagatgatt ataaagtttt gattgttgca aaaccaatgt
     1981 ttcctttcag tgaaaaagat aaattcatta tagatcaata cattatgaaa ggaggtcgta
     2041 ttctttggtt gattgacggg gtgcaacttt caaaagagaa tctttcaact gttggatttt
     2101 cgcctgcatg ggcattggat ataaatttaa acgatcaatt attcagatat ggtgttcgta
     2161 tcaatcctgt tctattgcaa gatgtacaat gtgttttggt acctgtaaat gttgcacctc
     2221 aagaccaatc tcctcacttc gaaccaatgc cttggtattt tgcacctttg cttctcacct
     2281 catatcaaca tcctattacc aaaaatataa cagaaattaa aacagatttt tgttctagta
     2341 ttgacgtagt aggtgacaat ccacatacaa aatcaacggt attgcttgct acttctaata
     2401 atactcatat tattcctaca ccttctacca ttgatttaag aatattacca aaagccaatg
     2461 ataaggaata ttttaattta gggtacgtgc cagtagctat tctggtggaa ggaagttttg
     2521 aatcggtttt taggaacaga atgcttcctg cgggtttgat aaatataaat aaggttcaat
     2581 atcaaagcaa accgactcgt cagatttttg tggcagatgg ggacatcatt agaaacgaaa
     2641 cttctgggtt agcaaccgat agtacaacat tatccttagg ttttgacaga tatatgaatc
     2701 aacaatttgg taataaagat tttattcaaa acgcagtgtt gtatttaacc gacgatgaag
     2761 gttggatgca attacgcacc aaaactttta agttgcgttt actaaataaa aatgctgtaa
     2821 atgataaaaa aaatatttgt caagtaatca atatttcagt tccaataatt ttattagctc
     2881 tttttggaat tatctatcag tggataagga aaagaaaata tagcaaataa aggggaaaat
     2941 aataaagaaa accataaata aaataaagat tgaatgccat aagtttgaaa aaggcgcctt
     3001 tggatttttt gccactgttc ataacaacaa tcattgataa taaaaaaatt gtaactccct
     3061 ccattattgt ggttattatt attcttaggt gattgatttt taaataattc cctttattgg
     3121 aactcttttt tggtaatttt ttatccgaac cacaagtatt tttttcattt tattattttt
     3181 taaaaaacca ggaaactttt ttatttttga agttttttaa ttaacttttt atttgattga
     3241 ttatcaatta tttataaatc attcagtaat tattaactat taataaatga ttttttgatt
     3301 tttggaataa gtttttttct aattttgcca aactttctaa ttatacatga aagacatacg
     3361 agagtccata ttacttgaag caagttttct atttcatcaa ttaggtataa aaaatatatc
     3421 tattgatgag atatgtagta atttgcgtat ttcaaaaaaa accttttatt cttactttga
     3481 tcaaaagcaa gatttggtag atgctgtgct tttctatcaa gaagaatttt atttgaaaaa
     3541 gctcgagggt attctaaaaa ataaaaatgc tattgagttt ctaattttat cattgaaaga
     3601 aatcaaaaaa agagcaaatt gtgagccaac actcatgtgg aatgatataa aaaaatatta
     3661 tcctgaagtt tttaaaaagc atattttgat aagacaagat tttctgaaaa aactttttga
     3721 aacaaattta aaacgtggtg ttgaagaagg tttttataga aaagatttag atataatttt
     3781 gatttcgatt ctttattccg aacacttaaa aaatattttt aataccttga ataaatattc
     3841 aaaaaaatat tcaaagaaaa gattgataga cttttttatt gacgtgatta ttcatgtaat
     3901 tgctaatgag gaaggattac aatatgttca ggagaattat aaaggtgtgg aagagaatcc
     3961 agtagaaatg aaaaagaatg aaaatatttt aaataattat agatcagaat caaagggagt
     4021 ttttaattaa attttagttg aattgaataa tttaaaagta taaaatatga ataaaaaaca
     4081 tttgaaaaga attttttttg ttgtgataag tttattattg attaatagta agttatatgc
     4141 tcaagataca cttatcgtaa ctttatcaga tgccattgaa attgccctga gcgaaagccc
     4201 tactattaaa atagctgata aggaaattga acgggtagat tattcaaaga aagaaaaacg
     4261 gggtgcttta tttccttcca taaatgctgt gggaaattat tcgagagcaa taaaaaaaca
     4321 gaagttgttt ttctctttac ctggtatgcc accacgacct gaaggagtag aagtgggaca
     4381 agacaatacc tttagtggcg gtatttcagc ttctctccct attattgctc ctactttgtg
     4441 ggcaaccatt caaatgtcag agctggatgc tgaattagca ctcgaatccg ctcgttcttc
     4501 aaaactttct ttaattaatg aagtgtcaaa gtcttattat gctactttat tagctcagga
     4561 ttcttatgat gtgttcaaaa aaagttatga tattgctatt gaaaatgcaa aagttataga
     4621 aaacaagtat aaacaaggag tagtatctga atttgaatgg ataagagcag atgtacaggt
     4681 gaaaagtgca agtactaacc tcgtttcagc agaaaatgcc gtcactctca gtattttgaa
     4741 attaaaaatg cttatgggta ttgacatgtt tacaggactg aaagtaaaag ggaatttagc
     4801 agattatgaa gctacaaaat attcggatgt attggctatc gatactacta ccttgcaagc
     4861 caactctgat ttaaagcaat tcgatattaa aacccaacaa cttcaacaaa ctttaaaaat
     4921 tcagaaaagc actttaatgc caactctttc agcttctttt aattatcaat acatgacaat
     4981 ggcaaatgat tctgtggctt tttcaaatta tcattggttc ccaacctcta caggagcaat
     5041 tacacttgcc attcctcttt ttcaaggtgg ggaaaaaata aataaacaaa agcaaataaa
     5101 agttcaattg gatgaaatga aatatcaacg cgataactta aggcgaggct tggaattaca
     5161 agcaatgtcg tatttagata atatcaatca agcaattaag acaattgaaa ccaataaaga
     5221 aggattacgt caggctgaaa aggcattgct tatatcgcaa aaaatgtatg aagtgggagc
     5281 ttctacttat cttgatttgt caaatgcaga attagtctat acgcaagctg gtttagctta
     5341 taatcagtct atttataatt atttgtcagc aaaagctgat ttagaaaaac ttttaggaaa
     5401 ataaaatata aagaattaat tagattttta aacaattaaa taatcaatat tcaaaatgaa
     5461 aaggacaata gaatttggta ttctgttttt agcaatagta tttggattta cttcatgcgg
     5521 aaaatcagga caaaagaaaa ccaaaattga aagtgaaatt ccaaatgttc gtattcagca
     5581 ggtgtcattg cgggatgttg aacgatatgc caacttaaca gcaactatct tgcctgaagt
     5641 caaaaataat gttgcaccgg ctgcaccagg tcgaatccga aaaatattcg tcgaagtagg
     5701 tgatcatgtt tcaaaagggc aaaaattagt tcagatggat gatgcaaatt tatcaaattt
     5761 agaaaatcaa gttgctaatt atcagcggat ttataatcgt gttttagaac tttttaatgt
     5821 gggtggtgct tctcagcagg aattggataa tgcaaaattg caacttgatg tagcaacgac
     5881 caatttgcaa aatttggccg aaaacactta tttgttaagt cctaccaacg gagttgttac
     5941 tgctcgaaat tatgataatg gagatattta tagtgctcaa tttcctgtgt tagttgttat
     6001 gcagataaat ccggtaaggg caattgtaaa tgtttctgaa acaaattatt cagatgtaaa
     6061 attaggaatg cctgctcaag taacattcga tattttaaaa aatgaatttt ttactgggaa
     6121 agtgaactta atttatccta ctattgatga aagaaccaga actttttctg ttgaaatcaa
     6181 attaaataac aatgattaca aaattcgtcc tggtatgttt gcaagggtaa aattaaattt
     6241 tggcacagta aaatctattg tagttcctga tcaggcagta attaagcaaa gcggttcagg
     6301 tgaatatttc gtttatattt ataaagatgg gaaagtgacc tatcaaaagg tagaattagg
     6361 gcagaaaatt ggaaatgaat atgaaattat ttccggtata gaagatgggg cacaagtagt
     6421 tgttgcagga caagctcgcc ttgctgatgg agtaaaagta aatgtagtca aataatgatt
     6481 attgaaaaaa taaactgagc aaagtttttt aaataacgaa gttctaaatt taaaattatg
     6541 agtttatacg aaagttctgt taaaagacct attatgactt ctctgatttt tattggggta
     6601 gttattttag gattgttttc attgagtaaa ttacctgttg atttattgcc aaatgtagaa
     6661 ccaaatatct taatggtagt tactaattat tctggagcga gtgctttaga tattgaaaca
     6721 aatgtaactc gtcctttgga aaacgtatta aatacagttg aaaatttaaa aaatataact
     6781 tccaattcaa aggaaaatac ttccattatt attatggaat ttgaatttgg tcaagatttg
     6841 gatgtagttt ccaatgatgt gcgtgataaa ttggaattag taaaacctta tttacctgaa
     6901 ggaactacca atccagtgat ttttaaattt agtaccgata tgatgcctat tgttattata
     6961 tcggctactg cagataagag tattccagct ctttataaaa ttttggatga taatgtagct
     7021 aatcccttag ctcgtatcaa aggagtaggt gctgtgtcta tttcaggtgc tcctcaacga
     7081 gaaatacaag tgtatattga cccggtaaaa ttggaagctt atcatttaac gattgaagct
     7141 gttggtcaaa aaatccgatt agaaaacatt aatacaccaa gtggatcaat ggatattggg
     7201 aataagactt attctttaag agtgcagggt gagtttaaag atccttctga attgagaaaa
     7261 gttgtagtag gaacttatgg caacaaaacc atttatttgt ccgacatagc tatcattagt
     7321 gatagtcatg aagaacgagt tcaagaaagc tataccaatg gtgtacaagg tgcaactata
     7381 aaaattcaaa aacaaacagg agctaatact gtagctattg taaatgctat ccgtgaaaaa
     7441 ttgcctgaaa ttcaaaaaac tcttcccagt gatattaaac taaacgtcat tgtagacagc
     7501 tccgataata ttaaaaagac tattgctgga ttggaagaaa ccattatgta tgcttttata
     7561 tttgtattgt tagtggtttt attcttttta ggaagatgga gagctacgat tatcattatt
     7621 ttgacaattc caatttcatt gatagcagca tttatttatt tagcagcaac aggtggaacc
     7681 ttgaatatta tttctttaag ttctctttcc attgccattg gaatggtagt agatgatgcg
     7741 attgtggtac ttgagaatat tactaaacat attgaaaaag gaagtagacc tacaagtgct
     7801 gctattcatg gcacagacga agttgctact gctgtggttg cttcaacctt aaccattgtg
     7861 gcggtatttt ttcccttcac cttagtatca ggtttagcag gaattctttt cgaccaatta
     7921 ggatggatgg taaccctcat tatgattgtt tctctggttt gtgccatggc tttaacacct
     7981 atgctaagtt cccaattgtt aactgtagaa aaaggtcatg gtaaattttt cactaaagtt
     8041 tattctccta tcgagcgttt cttaaataat ttggataatt ggtatgcagg attgattaat
     8101 tgggcggtac gtcacagatg gcaaatggta ttaattgctg ctggtttctt tatcttaagc
     8161 ttgattcctt tgataggcat tggaacggaa tttattccac cacaagacaa tgctcgtatt
     8221 tcagctacag tgcaattgcc tattggaaca agaatggaaa tttcaaaaga aaccgcagaa
     8281 gaattgacta aactttggag agaaaaatat ccagaaattg aaactattaa ttatactgtt
     8341 ggaccagcaa gtagcgataa cgtgttgggc tgggttatga caacagggac acatcttatt
     8401 tcttatactt ttcagttggt ggatgccaat gaacgagaaa gaagtatttt cgaaataggt
     8461 gatagcatcc gtcatgattt aaatatacgg cctgaaattg caaaatcaac tgttactgcg
     8521 ggaggtgcaa tgggaagaat tggagggcaa tccattattg acgtggagat ctatggttat
     8581 gattttaatc aaacagataa agtagcaaaa gaattgacta atcggatcga taagaatata
     8641 aaaggcttgg taaataccac gattagtcgt gaagattatt ccccggaata tcaagtcata
     8701 ttcgatagag aaaaattagc tttgaatggg ctggatattg ctacggcttc aacttttttg
     8761 agaaatagaa ttaatggatt agtagcaagc ctttatcgtg aggaaggaga tgaatataat
     8821 atccgggtag tttatgctcc tgaatatcgt gaaactattg aagatattga gaatattttg
     8881 atttataata atcaaggtca agcaattcgt ttgaaagatt tgggtaatgt ggtagaaaca
     8941 ttaactccac cttatattca aaggaaaaac agagagcgtg ttataactgt ttcaagtacc
     9001 gtttcaggaa caacaattga taaagcagtg agaggtttaa ggactcaatt atcccaaatg
     9061 gatattccta cgggaataga tgctgaaatt actggaactt atgaagatca gcaagaatca
     9121 ttcgctgatt tgggtacttt gttgattatt gtaattttac tggtatatat tgtgatggca
     9181 tctcagtttg aatctcttac ttatcctttt attattatgt ttgcgttgcc atttgctttt
     9241 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaagagtaa aaaaatagcc
     9361 agttttaaaa gcaatgtttt tactcaaact tatttaaaaa aattatttca tgaaatcaat
     9421 ttttattgtt tttgatcaag gtttttatca tcaagttctt gaaatacttt atatcaatca
     9481 tcatgttcgt ggatttacaa gctgggaaag ggttttggga cgtggcagca aaacaggtga
     9541 gcctcattat ggcactcatg cttggccttc attaaatggg gctatcttaa ccgttgttcc
     9601 agatgaaaaa gtaaaaccta ttttagaaga tttgaaagca ttggatgcca cttctgatgt
     9661 aatgggcata cgtgcttttg tatggaatat tgaagatgga atgtaaaatt ttagacttta
     9721 aaaaggcact ttgaatagtt tattataaaa agtgcctttt taatatgttt tatggtgtcc
     9781 acctatgaat tttctcaaag taattcggca attttattct ccaattttct taaatctttt
     9841 gtaaaattac gtattccttc tgctaatttt tccgtagcca tagcatcttc atttaacatc
     9901 catctaaaaa ttttttcgtc aattgtaata cgttgaacat cattgctttt tgctttttct
     9961 ggatcaagtt ttctctctaa tattccttcc gtttgttcca gctctttcaa taaagtggga
    10021 gagatggtga gtaaatcgca tcctgcaagt tcaataattt gttgaatatt tctaaaactg
    10081 gctcccatta tttgggtagg ataatcaaat tttttgtaat aattataaat tgacacaact
    10141 gattttacgc ctggatcttc gcttggagaa ttgaatgaaa aaccttcttt tttgtaccag
    10201 tctaaaattc gtcctacaaa tggtgatatg agagtgactc gtgcttctgc acatgctatt
    10261 gcttgtatca atgaaaaaag taaggttaaa ttacaatgaa tgccttcttt ttctaatatt
    10321 ttggctgctt caattccttc ccatgtcgag gcaattttta ttaatattct ttcccgtgaa
    10381 ataccttctt tttcatataa ggcgattaaa tcccgagctc tctcaatcgt tccccctgta
    10441 tcaaaagata aacgtgcatc aacttcggtt gaaactcgtc ctggaacaat ttccagaatt
    10501 tttattccaa aattaaccga taatttatct aatgccagct ttaattgttg tgatttatct
    10561 atatgagatt tttttgcata ttgaatggca tcaagtatta aatgttgata tttttcctgt
    10621 tgagaagcag caaaaatcag cgagggattt gtagtagcat ccaatggttt ataattaata
    10681 atagattcaa aatcgcctgt atcagcaact acttttgtat aagttttcaa ttgttctaag
    10741 agattcataa ccgtaaattc aagtaattaa tgcaactttt tcaggggaaa taaaaggtaa
    10801 aga
//
LOCUS       DAOSAS010000052        10752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4056_length_10752_cov_13.839114, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000052 DAOSAS010000000
VERSION     DAOSAS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4056_length_10752_cov_13.839114"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="PLR72_03720"
     CDS             complement(<1..1367)
                     /locus_tag="PLR72_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36350.1"
                     /translation="MKKKHFSLLIVFISTLLNCFILTPTRAQFTIIENFHGSSVGSNI
                     KVGGSAYLTSGIDDPVGEGWLRLTKDLGFQKGYAYIDSSFPSTLGVLIDFEYKTWRAS
                     LTDVGGDGFTVFLFDATVPFLEGGYGGSLGYAPFTGVSDGLAGGYIGIGFDEFGNFSN
                     PTQGRIGGPGQMCNSIVLRGPTTNDPATTNIYLTGVQLQTDPLSNINSIDYNTPTYDR
                     PSDDLFYRRVKISIEPIGTIQNPNYKITVKWRTTPTGNDITLLTYNTITPPPPNLKVG
                     FAASTGGSVNCHEIRDLVITTPGGMRVDKTVDKLNAKVGDQLTYKIDVYNSTNYSVNH
                     VNLTDTIKDANNKVIDIDSGIFTINSITFNNNGNAANTAEGFTSGIPVTSGLTNPFRI
                     NELSFQPNTMSTFIVKVTLKEIPPGGIFKNTVVVNVDPLLTGITDEDLTNNTFTVSTN
                     VLNTD"
     gene            complement(1539..3272)
                     /locus_tag="PLR72_03725"
     CDS             complement(1539..3272)
                     /locus_tag="PLR72_03725"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK36351.1"
                     /translation="MDYLSKKVVMTSVCSLLFVLFAFNAKGEEFAGQTTAGTYEISSA
                     ETLQLLKDFVNGTGSVAPYNAKHQCGGYTFMQTADIDLTGQSFAPIGNSGYRFKGTYD
                     GGNYKITGLSIGASGSPVSADGVGLFGYVDTGGALKNIVLEGGSVYANNIAGSLTGGI
                     NTGTIDNCHSSCMVNGLGNVGGLIGKIEGNTTVSNCSSSGEVQPQSTSSNALGGLIGT
                     YSGSGAAPKVYNCYSTSNVSAKYQVGGFIGNCSGSGIIENCYATGNITITAENKGGGF
                     IGDLSGTVVVKTCYATGNVIASTAGRNLLGGFAGDIRESNNVQNCYARGDVNGNQNIG
                     GFAGHIARATVQNCYATGAVTGVDNVGGLVGYLEENNSTSAIKNSVALNPSITRGSGT
                     NNTFGAAIGKTTVTDATRYSVNYSFNEMALPSGTLTGLACNQISKEDINLARNWWDIS
                     SSTGYYATIGTVNPPDVWIFADHKLPILKNGFFVNQSDAMPAHLLMSSDQVIQPASGQ
                     QNIFKISNGHLMIDENVEVTALRIYNVEGKVVLSDVSTARVIDISHIQSGVYIVKAIT
                     NKGTKVAKIVK"
     gene            complement(3468..3683)
                     /locus_tag="PLR72_03730"
     CDS             complement(3468..3683)
                     /locus_tag="PLR72_03730"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HOK36352.1"
                     /translation="MSPYKERIHTITSDNGKEFAEHKMISSELKVDFFFAKPYHSWER
                     GANKNTNELIRQYFSKKFLLKISLSRI"
     gene            4595..4738
                     /locus_tag="PLR72_03735"
     CDS             4595..4738
                     /locus_tag="PLR72_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOK36353.1"
                     /translation="MYRGSIRAFFYQGFARIFGANLKSKYDAYQEDKILERVIILVEA
                     GQI"
     gene            4798..5343
                     /locus_tag="PLR72_03740"
     CDS             4798..5343
                     /locus_tag="PLR72_03740"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOK36354.1"
                     /translation="MLDEKQQIRVHIRQLLKSLSDSEKTKDAEKVFSIIENMPEFQQA
                     EKILLYWSLPDELPTHFFVEKWSKEKQIFLPSIKNAEVVIKLFIDTHQMKPGLFNIAE
                     PDNEYEFNDDINLIIVPGMAFDKAKNRLGRGKGYYDRFLSCTKAIKWGVGFDCQLLEK
                     IPTNHFDIKMDKIIVPSTIIE"
     gene            complement(5351..6064)
                     /locus_tag="PLR72_03745"
     CDS             complement(5351..6064)
                     /locus_tag="PLR72_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36355.1"
                     /translation="MPYRRLPNTDQARLKALKTALEKLENQNFTHQIVPFKTVHEAKS
                     FLNIFETQLLQYRNALHNQTTASKQYQKNMATARLYISHFIQVLNLAVVRGEIKKEQK
                     QLYQLNPDSENVPALNSEKALLHWGKCIIEGENERVRAGGFPIYNPSIAKVKVYYDIF
                     KDNKLNQKFYQETTRRNWEELVALRPQADRLIVEIWDHVEAAFKEEKPFTRYQKCKEY
                     GLIYYYRKGEEKLTPDEES"
     gene            complement(6092..7066)
                     /locus_tag="PLR72_03750"
     CDS             complement(6092..7066)
                     /locus_tag="PLR72_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOK36356.1"
                     /translation="MFTHEEIIQLINQELQHLNWNKKPYGLYEPVEYVLSLGGKRLRP
                     ALLLLSCNLFSENISEAIPPALGIEIFHTFTLLHDDLMDKADLRRGHLTVHKKWDENI
                     AILSGDVMQTIAFQLMTKAPSACLKKCLDLFSQTAIEVCEGQQYDMEFEKRQEVLPEE
                     YLEMIRLKTAVLLGASMKIGAWLGGAPEEDAQTLYEAGINMGIAFQLKDDLLDVYGNS
                     AEFGKKIGGDILCNKKTFLLIQALNLARGEEEKILKKWLQVDENRGEEKVKAITAVYN
                     QLGIKEICENEIQFFSDKALEKLHQISVPENKKSELLKLTRQLVLRNE"
     gene            complement(7073..8056)
                     /locus_tag="PLR72_03755"
     CDS             complement(7073..8056)
                     /locus_tag="PLR72_03755"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOK36357.1"
                     /translation="MKKNIAIVAGGDSSEVGVSLKSAEGLYFYMDKNRYNVWIVTLVG
                     KNWQVQCLDGEKREIDKNDFSFTQQEKKIKFDFAYITIHGTPGENGLLQGYFNLIGLP
                     FSCCDVLPAALTFNKFYCNRFLKNFDILVAESLLFRKGALISIEEITQKIGYPCFVKP
                     NASGSSFGITKVKSENQIQLALDIAFTEGDEVMVEKFIEGTEVTCGIYKTKQKTVVLP
                     ITEVVPKNDFFDYNAKYNGQVQEITPARIQQQTAEQIQQLTTKIYDILNCKGIVRMDY
                     IISNEGAIYLLEVNTTPGMTETSFIPQQVKAAGLSMTDVLTEIIENELNNQ"
     gene            8276..8348
                     /locus_tag="PLR72_03760"
     tRNA            8276..8348
                     /locus_tag="PLR72_03760"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8309..8311,aa:Lys,seq:ctt)
     gene            8767..9186
                     /locus_tag="PLR72_03765"
     CDS             8767..9186
                     /locus_tag="PLR72_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOK36358.1"
                     /translation="MVQHTTKTIWRKNNIFDTEVDGHLITTDLAKEAGGDDAGPRPKK
                     LMLVAAAGCSGIDVVSLLKKMRVDLKGFDIQIEAEVSEEDPKQYTSMKVIYEFEGEEL
                     PMEKLEKAVKLSYEKYCGVMALYKKAIPVAYEIRIKN"
     gene            9307..10569
                     /locus_tag="PLR72_03770"
     CDS             9307..10569
                     /locus_tag="PLR72_03770"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOK36359.1"
                     /translation="MQTIDSFNFAGKKAFVRVDFNVPLDENFNITDDTRIRAAIPTLK
                     KILNDGGSVIIASHMGRPKKNPDPKYSLKHILPRVSELLGINVKFAPDCIGEETAKMV
                     AELKPGEALMLENLRFYEEEEGKPRVPETATEEEIAIAKKELKERQKKFTQTLASYAD
                     VYVNDAFGTAHRAHASTALIADYFDENHKMFGYLMEKEVAAIEKIMNNAEHPFTAIMG
                     GSKVSSKIDIIENLLNKVDNLIIGGGMTYTFIKALGGKIGDSICEDDKLELALELLEK
                     AKNKGVNMVLAVDTKIADSFSNDAHTKVVPVNEIPDGWQGLDIGPETEKIFSDIIKSS
                     KTILWNGPVGVFEFDNFAHGSLAIAQAIVEATKNGAFSLVGGGDSVACINKYGLADKV
                     SYVSTGGGALLEAIEGRVLPGIKAIRGY"
BASE COUNT         3614 a         1876 c         1663 g         3599 t
ORIGIN      
        1 aaatctgtgt ttaatacatt ggtcgatacc gtaaaagtat tattggttag atcttcgtcg
       61 gtgatacccg ttaacaaagg atcaacattt accacaacag tatttttaaa aatgcctccc
      121 ggaggaatct cctttaaagt tacttttaca ataaaagtag acatagtgtt cggttgaaaa
      181 cttaattcat taattcgaaa aggattggtt aaaccactgg tgacaggaat accactcgta
      241 aaaccttcgg ccgtattagc cgcatttcca ttgttattaa aagtaatact attgatagta
      301 aaaattccgg aatcaatatc tattacctta ttattggcat cttttattgt atcggttaag
      361 tttacatgat tgactgaata attcgttgaa ttataaacat ctattttata agttaactga
      421 tctcctactt ttgcatttaa tttatcaaca gttttatcaa ctctcattcc acccggcgtg
      481 gtaatcacaa gatcccgaat ctcatgacaa ttaacactcc cgccagtgga agcggcaaat
      541 cctactttta aatttggcgg tggaggagtg atggtattat aggtcaagag ggtaatatca
      601 tttcctgttg gagtggttcg ccattttact gttattttat aattaggatt ttgaatcgtt
      661 cctatcggtt caattgaaat ttttacccgt ctgtaaaata aatcatcact gggtctatca
      721 taggtaggtg tattataatc aatagaattg atattggata aggggtcggt ttgaagttga
      781 actccagtta aatatatatt agtggttgca ggatcatttg tagtaggtcc tcgaagaaca
      841 atagaattac acatttgccc tggtcctcca attcttccct gggttggatt ggaaaaatta
      901 ccaaactcat caaatcctat tccaatatat ccgccagcca aaccatcaga aactcctgta
      961 aaaggtgcat aacctaatga accaccgtaa cctccttcta aaaaaggcac cgtggcatca
     1021 aataaaaaaa cagtgaaacc atcaccccct acatcggtaa gagaagctct ccatgtttta
     1081 tactcgaaat ctatcaagac acctaacgta gaaggaaacg aagaatctat ataagcatac
     1141 cctttttgaa aaccaaggtc ttttgttaac ctaagccaac cttcacctac tggatcatca
     1201 atgcctgacg tcagataagc acttcccccg acttttatat tgctgccaac agaacttcca
     1261 tgaaaattct caattatagt gaactgagct ctggtagggg ttaatataaa acaatttaat
     1321 agggtagaaa taaaaactat taataatgaa aaatgttttt ttttcatttg agaataatta
     1381 agaacttttt tattttcttt tttattttct tttcagcact ttctcaactg cgaaacttca
     1441 atttattact gtaagaagtt caccggattt tccccggtga acttctttcc aaacaccaat
     1501 ctttgtttct caaaaacaaa acaccactat gctttctctt attttactat cttggcaact
     1561 ttcgtcccct tattggtaat agcttttaca atgtaaacgc ccgactgaat atgggaaata
     1621 tctattaccc tggctgttga tacatcagaa agtactactt ttccttctac attataaata
     1681 cgaagggcgg taacttctac gttttcatct atcatcaagt gcccattgga tatcttaaat
     1741 atattttgct gtccagaagc tggctgtata acttgatcac ttgacataag taagtgagca
     1801 ggcattgcat cgctctggtt aacaaagaaa ccatttttca gaatcggtaa tttatgatct
     1861 gcaaatatcc aaacatcagg aggatttact gttccaatgg ttgcataata acctgtagaa
     1921 gaagaaatat cccaccaatt gcgagctaaa ttaatatctt ctttacttat ctgattacat
     1981 gccagacctg ttaaagtgcc agaaggtaaa gccatctcat taaagctata atttacacta
     2041 taacgcgtag cgtctgtaac agtggtcttc ccaatagctg caccaaaagt attatttgta
     2101 ccacttccac gagtaatact tggattgagc gctaccgaat tttttattgc tgaagtactg
     2161 ttattttcct ccaaatatcc tactaagcca cctacattat ctacccctgt tacagctcca
     2221 gtagcataac aattttgaac ggtagctctt gcaatatgtc cagcaaatcc tccaatgttt
     2281 tgatttccat taacatctcc tcttgcataa caattctgaa cattattaga ttctcggatg
     2341 tctccagcaa atcctcctaa aaggttccta cctgctgtag aagctataac attccctgtt
     2401 gcataacatg tttttacaac aactgttcca gataaatctc ctataaatcc tcccccttta
     2461 ttttcagcag ttattgttat attacctgta gcataacaat tttctatgat tccactacct
     2521 gagcaatttc ctataaaacc ccctacctga tatttagcac ttacattact tgtggaatag
     2581 caattatata cttttggagc tgctccacta ccactataag tcccaattaa tcctcccaaa
     2641 gcattacttg atgttgattg aggttgaact tcaccgcttg aagaacaatt ggataccgta
     2701 gtattcccct caattttacc tatcaaacca ccaacattcc ctaatccatt gaccatacag
     2761 gaactatggc aattatctat cgttccagta tttataccac ctgtaagcga tcctgcaata
     2821 ttattggcat aaacagaacc accttccaaa acaatatttt taagagctcc tcctgtatca
     2881 acatatccaa ataagccaac accatctgct gaaactggtg aaccagaggc accaatgctt
     2941 aaacccgtaa ttttgtaatt gcccccatcg tatgttcctt taaaacgata gcctgaatta
     3001 ccaataggag caaaactttg accggttaaa tcaatatctg ctgtttgcat aaaagtataa
     3061 ccaccacatt gatgtttagc attgtaagga gctacacttc ccgtaccatt tacaaaatcc
     3121 tttaaaagct gtaaagtctc tgcagaagaa atttcataag tccctgcagt agtttgtcct
     3181 gcaaattctt cccctttcgc attgaaagca aaaagaacaa aaagtaatga acatacactt
     3241 gtcattacta ccttttttga taagtaatcc atttttttca taataataaa ttttaattgt
     3301 tattaaaata atttttttta ataaaaaata aataaatttt tcaagcaaaa ataaaatttt
     3361 aaaaaactta aaacaaattt ttcacctcac aaaaactcaa catactttaa ttttaactac
     3421 aataaaaaca caacataaag taatattcaa aatatcttct acccgcttta tatcctgctc
     3481 agagagattt tcaaaagaaa tttttttgaa aaatactgac gaataagttc gtttgtattt
     3541 ttgttagctc ctctttccca tgaatgatag ggtttggcaa aaaagaaatc caccttcaat
     3601 tctgagctga tcattttatg ttcggcaaac tccttgccgt tatcggaggt gatcgtatga
     3661 attctttcct tatagggaga caatcgttct atgactttca gggcaagttt tttggcatcc
     3721 ttgccattga gtttttcaat tattcaactt tcgcaagctc tattagcctg cttgcgtcaa
     3781 gctatcaaaa tttaagtatt tttaaatatt ctcattttca gaaaataatt tttacattaa
     3841 cgattcaatt tctatatcca aaatttcgga caaattggct atcaatcctg taattatcag
     3901 ctatatacgt tctgctactg taagtttgaa catatctttc ctatgattta atcatataaa
     3961 atttaaaaat tgattacaca atttttttta tggtgagaaa atgtgtataa ttcaagaaga
     4021 acattatata aaaagaaaaa ctttcgtcga agcggatgat gattttttga aattgattaa
     4081 ttaactaaaa ggtaataagg ttataatcaa gaagatattt caaagttatt aaggttaaat
     4141 gtttttatat aaggttttat taaaatttaa acaaattgct gacgtgagat gattttggac
     4201 ggtaaaatag aatttttctc tgtaaaattc agtgaaaatg aacggttatt acactataat
     4261 gtaatggtca aaatgttaat tcagtagaga cgccatatat gacgtctcta ctgaaatgcg
     4321 ttgaatgaca gttgattatt cacaatgaat aaatactggt aattatttag ttcaatgaaa
     4381 cagggttaat ttttcaacct tatcatgcgc taaaaacctt aataacaaga gaaaaaacaa
     4441 ataaaccaaa ccttattagc ttatgtataa agtttaaaaa ttgattgcat aatatttagt
     4501 gttattcaat cgagatttta aaaaaccata atatttattt gcaatagctt tatttcagaa
     4561 atagtttgta taatcacaat aaaatccata taaaatgtat cgaggaagca ttcgtgcctt
     4621 tttttatcaa ggatttgccc gcatctttgg agccaattta aaatcaaaat acgatgctta
     4681 tcaggaagat aaaattctcg aaagagttat tattttagta gaagcagggc aaatataaaa
     4741 cgaaaattta atgatagttt atttagcaaa agaaaatatt ttcttttttc agaaaaaatg
     4801 ttagacgaaa aacaacaaat cagagttcat atccgccaac ttcttaaatc attgtctgat
     4861 agcgaaaaaa cgaaggatgc ggaaaaagtg ttttctatta tagaaaacat gcctgaattt
     4921 cagcaagcag aaaaaatttt gctgtattgg tctttgcctg acgaattacc aactcatttt
     4981 ttcgttgaga aatggagcaa agaaaaacaa attttcttac cttcaataaa gaatgctgaa
     5041 gttgttatta agttgttcat tgatacccac caaatgaaac caggtttatt caacattgca
     5101 gaacctgaca acgaatatga atttaatgat gatattaatt taattattgt accagggatg
     5161 gcatttgata aggctaaaaa tcgtttggga cgaggaaaag gttattacga ccgtttcctt
     5221 tcatgcacaa aagcaataaa atggggcgta ggtttcgact gtcagttatt agaaaaaatt
     5281 cctacaaatc attttgatat aaaaatggat aaaattattg ttccttcaac gatcattgaa
     5341 taataacagc ttagctttcc tcatcaggag tcaatttttc ctcacctttc cgataataat
     5401 aaatcaatcc atactcttta catttctgat agcgtgtaaa aggtttttct tctttaaaag
     5461 ctgcttctac atgatcccaa atttcaacaa ttagtcggtc tgcttgcggg cgaagagcca
     5521 ctaattcttc ccagtttcgg cgagtagttt cctgataaaa tttctggttc aatttgttat
     5581 ctttaaaaat atcataataa acttttactt ttgcaatact gggattataa atagggaatc
     5641 ctcctgctcg aacacgttca ttttcacctt cgatgataca tttaccccaa tgaagcaaag
     5701 ctttttcact gtttaaagca ggtacatttt cagaatcagg atttaattga tacaattgtt
     5761 tttgttcttt ttttatttct ccgcgaacga ctgccaaatt caatacttga ataaaatgtg
     5821 aaatgtataa acgtgctgtt gccatatttt tttgatattg tttgctcgca gtggtttgat
     5881 tatgcaatgc attccgatat tgaagaagtt gtgtttcaaa aatatttaaa aaactttttg
     5941 cttcatgcac tgttttaaaa ggaacaattt gatgagtaaa attctgattt tccagctttt
     6001 caagtgcggt ttttaaagct tttaatcttg cttgatctgt gttaggtaat cggcgatagg
     6061 gcattttcga ttgaatttat ttatgagaaa tttattcgtt tctaagcaca agctgacgag
     6121 tcaattttaa taattcactt tttttatttt caggaacaga aatttgatga agtttttcaa
     6181 gagctttatc tgaaaaaaat tgaatctcat tctcacatat ttctttaatt cccaattgat
     6241 tataaacagc cgttatagct tttacttttt cttctcccct attttcatct acttgcaacc
     6301 atttttttaa tattttttct tcttcgcctc tggctaaatt taaagcttga ataagtaaaa
     6361 aggttttttt gttgcaaaga atatcacctc caattttttt cccaaactct gccgaattgc
     6421 cataaacgtc cagcaaatca tctttcaact ggaaagcaat tcccatgttg atacctgctt
     6481 catataaagt ttgagcatct tcttcaggag ctcctcccag ccaagcacct attttcatcg
     6541 aggctcccaa taaaactgcc gttttgagac gaatcatttc aagatattct tcgggaagta
     6601 cttcttgccg tttttcaaat tccatatcat attgctgacc ttcacatact tcaatggctg
     6661 tttgtgaaaa taaatctaaa cattttttta aacatgccga aggtgctttt gtcattaatt
     6721 gaaaagcaat ggtttgcatc acatcaccag aaagaatagc tatattttca tcccattttt
     6781 tatgcacggt aagatgccca cgtcgcaagt cagctttatc catcaaatca tcatgcaata
     6841 aagtgaaagt atgaaaaatt tctattccca aagcaggtgg aatagcctca ctaatatttt
     6901 cagaaaataa attgcatgac agtagcaaaa gtgcaggacg aagacgtttc cctcctaatg
     6961 aaagaacata ttcaacaggt tcataaagtc catacggttt cttattccaa ttcaaatgtt
     7021 gcaattcttg gttaataagt tggatgattt cttcgtgggt gaacatgttt ttttactgat
     7081 tatttaattc attttcaata atttccgtca aaacgtctgt catacttaat cccgctgctt
     7141 ttacttgttg tggaataaaa ctggtttctg tcataccagg cgttgtattt acttccaaca
     7201 aataaatagc accttcgtta gaaataatat aatccattcg tacaattcct ttacaattta
     7261 aaatatcgta aatttttgtt gttaactgtt ggatttgttc tgctgtttgt tgttgaattc
     7321 gtgcaggggt aatttcctgt acttgtccgt tgtatttggc attgtaatca aaaaaatcat
     7381 tttttggaac tacttcagta atgggtaaaa ctactgtctt ttgttttgtt ttgtaaatgc
     7441 cacaggttac ttctgttcct tcaataaact tttctaccat tacttcatct ccttcagtaa
     7501 aagctatatc caaagcaagc tgaatttgat tttcactctt tactttggta attccaaagc
     7561 tgcttccact tgcattgggc tttacaaaac aaggatatcc aattttttga gtaatttctt
     7621 caatggaaat aagtgccccc tttctgaaaa gtaaagattc agcaacaaga atgtcgaaat
     7681 ttttcaagaa gcgattacag taaaatttat tgaaagtaag tgcagcaggc aatacatcac
     7741 aacaagaaaa gggcaaacca attaaattga aatatccttg caaaagtcca ttttcgcctg
     7801 gtgtaccatg aatagtaata taagcaaaat caaattttat ttttttctct tgttgagtaa
     7861 agctgaaatc atttttatca atttctcttt tttctccgtc caagcattgc acttgccaat
     7921 ttttccctac taaagttaca atccaaacat tgtatcgatt tttatccata taaaaatata
     7981 atccttcagc acttttgagc gatacaccaa cttcggatga atcaccacct gcaacaattg
     8041 ctatattttt tttcataaca ttatcacata aaataagttt atcctatttt ttaattctac
     8101 aagataaaaa tgccactaca aaaataagta ttttttagag gcaataaaat gtaaaagaaa
     8161 gaaagaaata taaaatttaa aacaaccgaa tatctatcga aaaggtattt gaatctctct
     8221 tatagaattt tcaaagtagc aaataaaaat gtatctttgc acttgaaaaa acattgactc
     8281 cgtagctcag ttggcagagc aattgactct taatcaatgg gtcgagggtt cgagcccctc
     8341 cggggtcaca aaaaataaaa taaaattttg attttaggtg attgtataaa agcattatca
     8401 cctaatttta ttttgtaaga ttctttacag aaatattgac agcaaaaact gtaaatgaaa
     8461 taaaatactt acgaataatt tccaaaaaaa attctaaaaa tctttcaaat taaagaaatt
     8521 ccttaaaatt gactatcctc gccgcaagca gcgaggtatt acgccaattt tatggcttcg
     8581 ccaccggaat ttctaaaaaa ataaaaacaa aagctacccc aacgcaagca tcggggaatt
     8641 cttacgatta aaaaatatca tttcaaaatc aattattttt ttatcaattc taaagtaggt
     8701 tggttgaact aatttttttt aaaattgtta aaacataaaa cagataaatc ataaaatcaa
     8761 aaagctatgg ttcaacatac aactaaaaca atttggagaa aaaataatat tttcgataca
     8821 gaagttgatg ggcatcttat taccactgat ctggcaaagg aagcaggtgg ggatgatgca
     8881 ggtcctcgac caaaaaagtt gatgttagtt gcagccgcag gttgtagcgg aatagatgta
     8941 gtctctcttt tgaaaaaaat gagagttgac ttgaaaggtt ttgatattca aatagaagca
     9001 gaagtaagcg aagaagatcc caaacaatat acttctatga aagttattta cgaatttgaa
     9061 ggagaagagc ttcccatgga aaaacttgaa aaggcggtta aactttcata cgaaaaatat
     9121 tgtggggtaa tggctttgta caaaaaagca attccggtcg cttatgaaat aagaataaaa
     9181 aattaagaat tcataaaagt tttttcaaag gttgaaatac gaaccataat tttactattt
     9241 ttgcaaatgg ttctttgctg atttttctac aaaatcataa acattatttt taaatatttg
     9301 aagattatgc aaacaataga ttcttttaat tttgccggca aaaaggcatt tgtacgcgtg
     9361 gattttaatg taccactcga tgaaaatttt aacattacgg atgatactcg tattcgcgca
     9421 gcaattccca ctttgaaaaa aattttaaat gatggaggaa gcgttatcat tgcttcacac
     9481 atgggcagac ctaaaaaaaa tcctgatccc aagtattctt taaaacacat tttacctcgc
     9541 gtttctgaat tactgggaat aaatgtaaaa tttgcacctg attgtatagg tgaagagacg
     9601 gcaaaaatgg tagctgaact taaaccaggt gaagctttaa tgttagaaaa tttacgtttt
     9661 tatgaagaag aagaaggaaa acctcgcgta cctgaaacag ctactgaaga agaaattgca
     9721 attgcaaaaa aagaactaaa agaacgtcag aaaaaattta cccaaacatt ggcttcatac
     9781 gctgatgtat atgtaaatga tgcttttggg acagctcacc gtgcacatgc ttctactgca
     9841 ttgatagcag attattttga tgaaaaccat aaaatgtttg gctatcttat ggagaaggaa
     9901 gtagcagcca tcgaaaaaat aatgaataat gctgaacatc cttttactgc cattatgggt
     9961 ggctcaaaag tatcttcaaa aattgacatt atagaaaatt tactgaataa ggtagacaat
    10021 cttatcatcg gcggcgggat gacttatact tttattaaag cattgggtgg aaaaattggt
    10081 gattctattt gcgaagatga taagcttgaa ttggctttag aattgcttga aaaagcaaaa
    10141 aacaaaggcg tcaatatggt tttggctgtt gatactaaaa ttgctgattc attcagcaat
    10201 gatgctcaca caaaagttgt tcctgtaaat gaaattcctg atggttggca aggcttagat
    10261 attggtcctg aaacggaaaa aattttcagt gatattatta aatcatctaa aactatttta
    10321 tggaatggtc cggtaggagt ttttgaattt gataatttcg cacatggttc attggcaatt
    10381 gcccaagcta tagtagaagc tactaaaaat ggagcttttt cgctggtggg tggtggtgat
    10441 tcagtggctt gtatcaataa atatggtttg gcagataagg tttcttatgt atcgactggc
    10501 ggtggagctt tgctcgaagc tatcgaaggt cgcgtactgc caggtattaa agccatcaga
    10561 ggctactaaa tgaaaaaaaa tctattttta ggaaaaacgt caatgtcaga ttttaaatga
    10621 tattgacgtt tttttataaa ttatagtaat tttcacctta cagcgaatat ataaaaaatt
    10681 caaagttttt gttagcgtcc tgattttgta attaattctt taagataatg attaaagtcg
    10741 tcttctttta at
//
LOCUS       DAOSAS010000053        10654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4106_length_10654_cov_9.197188, whole genome shotgun sequence.
ACCESSION   DAOSAS010000053 DAOSAS010000000
VERSION     DAOSAS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10654
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4106_length_10654_cov_9.197188"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLR72_03775"
     CDS             <1..437
                     /locus_tag="PLR72_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK36360.1"
                     /translation="ATGYKIYVYQGSTLIEGAPFSVSGQTINSFDIMNLATSTTYSYK
                     VQAIGDGDINYSDSFLSSAIEFTTLVTGIDNLKNNPNITVVNKKIICPETGDMRIFNM
                     QGGLIESVKNVKTVQTDLPSGIYIVKFSNQNGETKIQKIAIQ"
     gene            790..1827
                     /locus_tag="PLR72_03780"
     CDS             790..1827
                     /locus_tag="PLR72_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK36361.1"
                     /translation="MKKLILLFSVGFAFFMAKAQEPIFTEYFETAPIGGDLEGYNDWH
                     VSPKSSEANGVSPKIAEGALFYTGYAGSNIGNVAVLDSAVGATSATQRISTKIVKFGS
                     DTLKAVVGEKIYTAFLSNISSHSYRSYRDFFTYEGSKTSSMTRGRVFAKVSTDGSELF
                     IAVTKNSTIATDYVESTSIPGLTLYPDVNHLFVLVYETIAGDSNDKITLYIDPDLTKS
                     ETEQTNKLVAIDTQTDYSASAGLGINLRQRGIGAQVGGIRVGKSWDAVLLGEPNALNI
                     PNINSPIWTNGKNIMTRQGGTIKVFNMAGAELISTQTNGCLSTNLTKGLYIIRFKGND
                     GATVISKISLN"
     gene            1916..3655
                     /locus_tag="PLR72_03785"
     CDS             1916..3655
                     /locus_tag="PLR72_03785"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HOK36362.1"
                     /translation="MNYLSNIIFRLTVILIFSMSCVMGNARQTVVVNASGINNGTWTA
                     GDTIVMKNGMWTNQSISFKATGIASQPVVLMAETPGKVILNGTSKFQFSGKYIVVSGL
                     YFKDGILSGSDVISFRTSSSQLAENCRLTNTVIENYNPSDNAIDSKWISIYGKNNKVD
                     HCSFVNKTNSGTLMVVWLTNGVTVNHEISDNYFGYRNPNLDANGNELNGQEIIRIGDS
                     STSMTTAGVIVSGNFFEHCNGEIETISNKSCGNIYTNNLFLECKGTLTLRHGNNCTVA
                     GNYFFGNGISNTGGVRIIGENHKVYNNYFENLRGTNYRAALCIVRGKENSALNEYFQV
                     KNATIAFNTFVNCSQSFRINYNSSSSFTMPPVETVIAHNHVYNTSSSYYNVSIDLTNI
                     TEMDVTWKNNLMNIGRYTNFPYDETQVITGKDAKMNLADTPIPIYEPSTGSELTNYTT
                     SDYPEITKDIRGRERNSMTKLPGASEITGIVTQSMPIKTLVGATFYNNPDTKIVKICD
                     LKPYNFQLNELHIIFNPSETGYFYVIDLSGKILFNTLVYAGTSFTTKILEKGIYILLF
                     KTPTSQYVEKIVI"
     gene            3759..5978
                     /locus_tag="PLR72_03790"
     CDS             3759..5978
                     /locus_tag="PLR72_03790"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOK36363.1"
                     /translation="MKKNIQIIENLFYLLIFLSFSFEAISIEHPNLLLTKQGVKEIQA
                     SLGKYPDFDQSVNQLKTIADNALKSEIIVPIPKDEGGGYSHEKHKNNYYEMNAASTMY
                     QLTGKKEYAEFVKQMLLKYAELYPAIGLHPSRKSNAPGKLFWQLLNDCVWLFHTAMAY
                     DCICDYLNTSDKVNIEKNLFRPMAEFISNGNAHNYEIFNKMHNHATWATASVGMIGYV
                     MEDKSLVEKALYGSNKDSKSGFIRQLDILFSPDGYYTEGPYYQRYAIWPFMVFAQVIQ
                     NNQPELKIFSYRDSILLKATNTLLQCAYNSEIFYLNDALKKTYQTQEIIYAVDIAYKN
                     YPKNTSLLDIARQQKSFLVADAGIATAKSLHNIKYEPFQFRSLLLRDGENGEKGGLGI
                     VRAGKKDKTQTCLTFKATSHGLSHGHYDKLSIVLYDNGNCIMPDYGAVRFLNIEPKNG
                     GHYTKENYSWAMQTVAHNTVVVDKTSHFNADIKISSNYHSDIQYYDFNNPDFQIISGC
                     ENNAYKNVKMQRTVAIIKNNNFSFPLIIDVFRVVADSTHTLDLPFYYRGQIVSTNFKY
                     QKFTTQLNPLGTKNGYQHLWVDAIGKNKKSTTHFTWLAGNRFYTISALGNTDTEFFMA
                     HSGADDPDFNLRNETCFMIRQPNVQNHTFVTVIEPHGFYDLNKEIIENLQTSISNIEL
                     LADNQEYTAIKISIINNKAFLFITLNSNFDKTSEHTINLDNQKFQFKGNYYFSALTK"
     gene            5990..6334
                     /locus_tag="PLR72_03795"
     CDS             5990..6334
                     /locus_tag="PLR72_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOK36364.1"
                     /translation="MKTRSLNFLHASEIEWEITGEGVSRQILGYDGQIMMVKVHFKKG
                     AIGYIHEHFHSQTTYVASGKFEVTINDEKQILEAGDGYYIEPDAPHGAVCLEEGILID
                     VFSPMRMDFLKK"
     gene            6339..7802
                     /locus_tag="PLR72_03800"
     CDS             6339..7802
                     /locus_tag="PLR72_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK36365.1"
                     /translation="MKIKGLRWWIIGLIVLITIINYLDRGTLNYMWVTNVEYTIVDQF
                     SNNLKTNEAIYLKDKNTYLLKNKKGKIIEQQATHLSQKKDGKVVVNREGIAYDLGIIE
                     KDTTPDEASKKAKGILGTITIFFMIAYGISQLFSGKMYDKIGTRRGFVISAAIWGLAD
                     ALTSLAKGKFSLTAFRIMLGLGEAGPWPGAVKSNAEWFPQKERAFAQGLFNAAASIGS
                     ILAPIVILMLYLLVGWRLTFLIVGSLGLIWIIPWLIINKKGPKEHPWITEKEREYILT
                     GQPEAQIVDERGKSWGELLSNKKNYSVILGRFFLDPIWWMFVTYLPLYLVEQFNLNIT
                     ELAFSAWVPYFGAMIGSLTGGWFSGYLMRHGKTVDKARKIAMLVGGLIIIPSVIASVM
                     VSNAMMAVILMAFVLGGFQFTMTNIQTLPSDLHCGKSVGSLAGLGGASAVLGTILAIL
                     FSAKIPSWPLLFGLLAALVPLSLGSIFLTVGKIEQIK"
     assembly_gap    7832..7931
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7932..8438
                     /locus_tag="PLR72_03805"
     CDS             <7932..8438
                     /locus_tag="PLR72_03805"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOK36366.1"
                     /translation="DKIDILVNVVGGIMGRKEITEQDENWYSALMDVNMKSCWLCTRE
                     VVPHMNQGGAIINFSSLAARDGGGPGASMYATAKGAVMTFTRSMAKELGPKGIRVNAV
                     APGTIATSFHDKFNTPENRERMKGIYPLRREGDADDVADLVLFLASSDSDYITGANID
                     INGGIYFS"
     gene            9737..10213
                     /gene="bcp"
                     /locus_tag="PLR72_03810"
     CDS             9737..10213
                     /gene="bcp"
                     /locus_tag="PLR72_03810"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOK36367.1"
                     /translation="MALQVGDKIPDYLGVDQNEKKIYSSNFKGKKLILYFYPKDNTSG
                     CTSEACSFRDNYAELRRRNFEIIGVSTDDILSHQKFILKNNLPFTLITDTDHRLGEVF
                     GTFKEKKRFGRKYFGIERTTFIIDENGIITHIIPQEEIDTKKHAEQILERVGDEEK"
     gene            complement(10304..>10654)
                     /locus_tag="PLR72_03815"
     CDS             complement(10304..>10654)
                     /locus_tag="PLR72_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36368.1"
                     /translation="NIEFNEINLRFKREVNSMTHLLPEIYRDAIKEDAQKVITQFQPD
                     IEKWLVRLKNKNMSCFEIESLLQSRLNEVSLNSLQQTCDESQTVEKIKNNILNLIAKS
                     VLNTYLYSMFKDVT"
BASE COUNT         3654 a         1544 c         1816 g         3540 t
ORIGIN      
        1 atgcgacagg ctataaaatt tatgtttatc aaggatcaac gttaattgaa ggagcacctt
       61 tcagtgttag tggacaaacc attaacagtt ttgatataat gaatttggct acatccacaa
      121 catattctta caaggttcaa gccatcggag atggggatat aaactattcc gattcatttc
      181 tttcttcagc tattgaattt acaacgctgg ttactggaat agataattta aaaaacaatc
      241 cgaatattac cgtagtaaat aaaaaaataa tttgtcccga aacaggtgat atgcgtattt
      301 tcaatatgca aggtggcttg atagaatcgg taaagaatgt caaaactgtt caaactgatc
      361 tcccttcagg aatttatatt gttaaattca gtaatcaaaa tggtgaaacc aaaattcaaa
      421 aaatcgccat tcagtagttt attcagcaag aatcatatcg gcattttttc gtgcagatat
      481 gattctttaa aatgttgctg gaagaaaaac ctaaattcaa gtaattaata caactttttt
      541 aagaaaataa taagtagaaa aaagagagaa aatttaataa aaagttgaga ttaataaatt
      601 ctttttaatt tttaaaagat taaaatcatt gaataaaagt tcttgtatta atttcatttg
      661 tctttcaaaa ttattctaat aaaatttgtt ttttattaaa atatacctta tttttggtcg
      721 accaattttt taaaatgaga attttttgta ataatttttt taatgtctaa cttttaattt
      781 ctttacatta tgaaaaagct aattttactt ttttcagtcg gttttgcttt ttttatggca
      841 aaagcacaag agccaatttt tacggagtat tttgaaacag cgcccattgg tggcgactta
      901 gaaggttata atgattggca tgtttctcct aaatcttcag aagcaaatgg agtgagtccc
      961 aaaattgctg aaggggcttt gttttacacc ggttatgcag gttctaatat tggtaatgta
     1021 gcagtattgg attctgcagt gggtgcaaca agcgctacac aaagaatctc tacaaaaatt
     1081 gtaaaatttg ggagcgacac tttgaaagct gtagttggag aaaaaattta tacagcattt
     1141 ttgtctaaca tttccagcca cagctatcgt tcttatcgtg atttctttac ttatgaaggt
     1201 tcaaagactt ccagtatgac acgtggaaga gtttttgcta aagtcagtac agatggttcg
     1261 gaacttttta tagccgttac aaagaattct actattgcta ctgattatgt tgagtctact
     1321 tctattccgg ggttaacttt gtatccagat gttaatcatt tatttgtttt agtttatgaa
     1381 acaatcgcag gtgatagcaa tgacaaaatt acgctgtaca tcgatccaga tttaacaaaa
     1441 tcggaaactg aacaaaccaa taaattggta gcaattgata ctcaaactga ttatagtgct
     1501 agtgcaggat taggtattaa ccttcgtcaa cgtggtattg gtgctcaagt tggtggtata
     1561 cgggtgggta aatcatggga tgctgttctt ttgggtgagc ctaatgcact caatatccca
     1621 aatattaact ctcccatatg gacgaacggt aaaaatatta tgacccgtca aggaggaacc
     1681 ataaaagtat ttaacatggc gggagctgaa ttaatttcta cacaaacgaa cggatgtctg
     1741 tcaactaatt tgacgaaagg gttgtatatc atacgtttta aagggaacga tggcgctact
     1801 gtcatatcaa aaatttcgtt gaactaattt ttttaatttc tcaactcatt attaaggaat
     1861 gcagacagaa tttttttctt ttgcattcct taaaaaatca ataaaattaa atttcatgaa
     1921 ttatctttct aatataattt tcaggctgac agtaatattg attttttcaa tgagttgtgt
     1981 aatgggaaat gccaggcaaa cggtagttgt caatgcttca ggtattaata atggcacatg
     2041 gacagcaggc gataccattg taatgaaaaa tgggatgtgg accaaccaat ctatttcatt
     2101 taaagccaca ggaattgctt ctcagcctgt agttttgatg gctgaaactc caggtaaagt
     2161 aatattaaac ggaacttcaa agtttcagtt ttcggggaaa tatatagtag ttagtggact
     2221 ttatttcaaa gatggaatat taagtggttc agatgttatt tcatttcgta cctcttcttc
     2281 tcaattggct gaaaactgca gattaactaa cacagtaata gaaaattata atccttccga
     2341 caatgcaatt gacagcaaat ggatatcgat ttatggaaaa aacaataaag tggatcattg
     2401 ttcttttgta aataaaacaa attcggggac tttaatggta gtttggttaa ccaatggtgt
     2461 aacagtaaat cacgaaatct ctgacaatta ttttggctat cggaatccaa atcttgatgc
     2521 aaatggaaac gaattgaatg gacaagaaat tattcgaatt ggtgatagtt ctacttctat
     2581 gacaacagcg ggagtaatag tctctggaaa ctttttcgaa cattgtaacg gtgaaatcga
     2641 aaccatttct aataaatctt gtggaaacat ttatacaaac aaccttttct tggaatgtaa
     2701 aggaacgctt actttacgac atggtaataa ttgtactgtc gctgggaatt atttttttgg
     2761 gaatggaatt tcaaatacag gaggcgtgag aattattgga gaaaatcata aagtttataa
     2821 caattacttt gaaaatctgc gaggtacaaa ttatcgggca gcactttgca tcgtgcgggg
     2881 aaaagaaaat tcggcattga acgaatattt tcaagtaaaa aatgctacca tagcttttaa
     2941 tactttcgtt aactgttcac aatctttcag aatcaattac aacagcagtt catcattcac
     3001 catgccacct gtggaaacag taatagctca taatcacgta tacaatactt cttcttcata
     3061 ttataacgtt agcattgatt tgactaatat tacagaaatg gatgtaacct ggaaaaacaa
     3121 tttgatgaat attggaagat acacaaattt tccttatgac gaaactcaag taataacagg
     3181 taaagatgca aaaatgaacc ttgctgatac tcctattccc atctatgagc catcgacggg
     3241 ctcagaatta actaattaca caacttctga ttatccagaa ataacaaaag atataagagg
     3301 aagggaaaga aatagtatga ctaaacttcc aggtgctagc gaaataactg gtatagttac
     3361 tcagtcgatg cccataaaaa ctttagtagg tgctactttc tataataatc cagacaccaa
     3421 aattgtgaaa atatgcgact taaaacctta caattttcaa ttaaatgaac tacatattat
     3481 cttcaatcca tctgaaacag gatattttta tgtgattgat ttgtcaggaa agatattgtt
     3541 taatacattg gtttatgccg gaacttcatt taccaccaag atcttagaaa aaggaattta
     3601 tatactttta ttcaaaactc caacatcaca atatgttgaa aaaatagtta tttaggataa
     3661 aatagttttt aataattatc aaaaagcatt gaattttaca attaaaaatg taaatttgca
     3721 aaacaaaaat tggtcgacca atttctaaat tgttaacgat gaaaaagaat attcaaatta
     3781 tagaaaattt attttactta ttgatatttc tttctttttc ttttgaagca atatctatcg
     3841 agcatcccaa tttactgttg acaaaacaag gagtaaagga aattcaagca tcactgggaa
     3901 aatatccgga ttttgaccaa tcagtaaatc aactgaaaac aatagcagac aatgcattga
     3961 aatctgaaat cattgtcccc atacccaaag atgaaggagg tggatactcc catgagaaac
     4021 ataaaaataa ttattatgaa atgaatgctg ctagtaccat gtatcagctt actggcaaga
     4081 aagaatatgc agaatttgtt aagcaaatgc ttttaaaata tgcagaactg tatcctgcga
     4141 taggtttaca tccttccaga aaatctaatg cgcctgggaa attattttgg cagttgttga
     4201 acgattgtgt atggttgttt catacagcaa tggcttatga ttgtatttgc gattatctca
     4261 atacttccga taaagtaaac attgaaaaga atctctttcg tccaatggct gaatttattt
     4321 ctaacggtaa tgctcataat tatgaaattt ttaacaaaat gcacaaccat gcaacgtggg
     4381 caactgcttc agtagggatg attggatatg taatggaaga taaaagtttg gtagagaaag
     4441 ctttatatgg ttccaataaa gatagtaaat cgggtttcat caggcaatta gatatactgt
     4501 tttctcctga tggctactat accgaaggtc catattatca acgctatgct atttggccat
     4561 tcatggtttt tgctcaagta atacaaaaca accagccaga attaaaaatt ttcagttacc
     4621 gagacagtat tttactaaaa gcaaccaaca cattacttca atgtgcttac aatagtgaaa
     4681 ttttttacct gaatgatgcc ttaaaaaaga cttatcaaac tcaggaaatt atttatgctg
     4741 tggacattgc ttataaaaat tatccaaaaa atacatcttt gcttgatatt gcacgacaac
     4801 aaaaatcttt tctcgtagca gatgctggta tcgctactgc aaaatcctta cataatatca
     4861 aatatgaacc atttcaattt cgctctttac ttttacgtga tggagaaaat ggagagaaag
     4921 gtgggttggg aattgtcagg gcaggcaaaa aagacaaaac acaaacttgc ctgactttta
     4981 aagctacttc tcatggttta tcacacggac attacgataa attgtcgatc gttttgtacg
     5041 ataacgggaa ttgcattatg cctgactatg gagcagtgcg gtttctcaac attgagccaa
     5101 aaaatggagg tcattataca aaagaaaatt attcatgggc aatgcaaacg gttgctcata
     5161 atactgtcgt agtagacaaa actagtcact ttaatgcaga cataaagatt tcatcaaatt
     5221 atcattcgga tattcaatat tatgatttta ataatccaga ttttcaaatt atatcgggat
     5281 gtgaaaacaa tgcttataaa aatgtaaaaa tgcagcgtac tgtggcaatt attaaaaaca
     5341 ataatttttc ttttccgctt attatcgatg tttttagagt agttgctgat tctacgcata
     5401 cactggacct tcctttttat tatcgaggtc aaatagtatc gacgaatttc aaatatcaaa
     5461 aattcacaac tcaattaaat ccattaggta caaaaaacgg ctatcaacat ctctgggtag
     5521 atgcaattgg aaaaaacaaa aaatctacaa ctcattttac ctggttagca ggaaacagat
     5581 tttatactat ttcagcatta gggaataccg atactgaatt ttttatggca cattctggtg
     5641 ccgatgaccc cgattttaat cttagaaatg aaacttgttt tatgattcga cagccaaatg
     5701 ttcaaaatca tactttcgtg acagtaatag aacctcatgg attttatgat ttaaacaaag
     5761 aaattattga gaatttacaa acctccattt caaatattga acttttagct gataatcagg
     5821 agtatactgc aataaaaatt tcaataatta acaacaaagc atttcttttt attactctta
     5881 acagcaattt tgataaaaca agtgaacata ccattaactt ggacaatcaa aaatttcaat
     5941 ttaaaggaaa ttattatttt tcagcattaa caaaataaat tttaagaata tgaaaacaag
     6001 aagtttaaat tttttacatg cttcggaaat agaatgggaa ataacgggag aaggtgtatc
     6061 acgacaaatt ttaggatatg atggacaaat catgatggta aaagttcact ttaaaaaagg
     6121 agcaataggt tatattcatg aacattttca ttcacagaca acttatgtgg cgagtggaaa
     6181 atttgaagta accattaacg atgaaaaaca aattttggaa gcaggtgatg gatattatat
     6241 tgaaccagat gctccacatg gagcggtatg tcttgaagaa ggaattttaa ttgatgtatt
     6301 tagtcctatg agaatggatt tcttaaaaaa gtaagataat gaaaataaaa ggattacgtt
     6361 ggtggatcat tgggctgatt gtgctcatta cgatcataaa ttatctggat agaggaacat
     6421 tgaattacat gtgggtaacc aatgtagaat ataccattgt tgaccaattt tctaacaacc
     6481 taaaaacaaa tgaagctatt tatttgaaag ataaaaacac ttatttatta aaaaacaaaa
     6541 aaggtaaaat aattgaacag caagctactc atttatctca aaaaaaagat ggtaaagtag
     6601 ttgtaaacag agaaggaata gcttacgatt taggtattat tgagaaagac acaacaccag
     6661 atgaagcttc aaagaaagca aaaggcatac tgggcactat tacgatattt tttatgattg
     6721 cttatggcat tagccagcta ttttcaggaa aaatgtacga taagattgga acacgtcgtg
     6781 gttttgttat ctctgcggct atttggggat tggcagatgc gcttacctca cttgcaaaag
     6841 gaaaattttc cctgacagcc tttaggataa tgctgggatt aggagaagct ggtccctggc
     6901 caggagctgt aaaaagtaat gctgaatggt ttcctcagaa agaaagagct tttgcacaag
     6961 gacttttcaa tgcagcagcc tcaattggtt ctattcttgc tcctatcgtt attttaatgt
     7021 tgtatttgtt agtgggttgg agattaacat ttttaattgt cggttctctg ggtttgattt
     7081 ggattattcc ttggttgatt atcaataaaa aaggtcctaa agagcatcct tggatcaccg
     7141 aaaaagaacg cgaatatatc cttacaggtc aacctgaagc tcaaattgtt gatgagaggg
     7201 gaaaaagttg gggagaatta ctttcaaata aaaaaaatta ttctgtcata cttggacgat
     7261 tttttcttga tcctatttgg tggatgtttg taacttattt acctctttat ctggtagaac
     7321 aattcaatct caatattaca gaattagctt tttcagcttg ggtaccctac tttggtgcca
     7381 tgattggtag cctgacaggt ggttggttct ctggatattt aatgcgtcat ggaaaaacgg
     7441 tcgataaagc acgtaaaata gctatgttgg ttggtgggct aatcattata ccttccgtca
     7501 ttgcttctgt aatggtatcc aatgcaatga tggcagtaat tttaatggca tttgtactgg
     7561 gaggatttca atttactatg actaatattc aaacgctccc cagcgattta cactgtggaa
     7621 aatcagtcgg ttctctggct ggtctaggag gtgcttctgc tgtactaggt accatattag
     7681 caatattatt ttctgctaaa ataccaagtt ggcctttgct atttggactt ttagccgcat
     7741 tagttccttt gtcattagga tcaatatttt taactgttgg gaaaatagaa caaattaaat
     7801 aacaattttt taaataataa atattatgaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn ngataaaatt gacattttag ttaatgtagt aggagggatt atgggcagga
     7981 aagaaattac agaacaggat gaaaattggt attctgcctt gatggatgtt aatatgaaaa
     8041 gctgctggct ttgtacacgt gaagtagtac ctcatatgaa tcaaggaggt gctataataa
     8101 atttttcatc gttagctgca agagacggtg gaggaccagg agcatcaatg tatgcaacag
     8161 caaaaggagc ggtgatgaca tttactcgtt ccatggcaaa agaactggga ccgaaaggta
     8221 ttcgtgttaa tgcagttgct ccgggcacaa tagctacctc cttccatgac aaattcaata
     8281 cacctgaaaa tcgcgaacga atgaaaggta tttatccact tcgtcgggaa ggtgacgcag
     8341 atgatgtagc tgatttggtc ttatttctgg cttccagtga ttcagattat attacagggg
     8401 caaatattga tattaatggg ggaatatatt tttcttaatc tgttattccc aatttatttt
     8461 tacaatctga gttttgaatg aaaaatagta atataaagtt gcaaaatagc gtaattattt
     8521 tgcaacttta taattgaaaa tcaaataatt tataaataat atcttgtaga taaattttta
     8581 aattttttta tattatcact aacgattttt gtaatgaaca ggagaataaa gaaattcaag
     8641 aatcactttt cgcaattcta ttctattttt atttgcaaat gaaagaagaa attattcttc
     8701 ttttagattt tgttttatga accaattcat aagcctcgta taatttttta tacccgtttc
     8761 agaaagtctt tagaaccttt ttggtcgttt tcaaagcttg cttgtactaa aaacctattg
     8821 atttataacc tatagtatgt tacatcgaac gtatttttag aaaagtcaat tctaacaaat
     8881 aaaaaaaaat ttttcatact tttgtatttt ataggtttta aatttggaat taaccgataa
     8941 aaactgcttt gtactaaggc ccttaatggg cttttaatct taatattcta tttggtattt
     9001 tgcagtttga ttcttaaaag tcttgtaccc tcacgtagaa ttaaatttct atgtctctac
     9061 atagttcact tttaagaata tacggttaat ttcttctttt ttaccggatt ttcctttcaa
     9121 aagtggtata ttttatatat aacaaattga ttattatttg tttaatatgt ttttttaaaa
     9181 taatttcttg cttacagtgt aattattagt tgtttatcac atattgaata ttattttatt
     9241 tttacaaaca tacaaaaaaa ttcaataaaa taactttgtt agaagaaaaa aatagtattt
     9301 ttcttgcgta cattaaattt actggaatat tcttaaaagc cgattggtat tggcttttag
     9361 atcatttttt acaaaatttg gaagggaaaa tccgttacaa caaaagtaaa gaattaactt
     9421 ttaaaattta ttctaaaaat tagactcgcg aactaagagt ctctacttga aattcgaaaa
     9481 tccaaactcc tgaccaccga ctattctttt attatttttt atttttaaag ttgcttggta
     9541 taagtttttc tttggatatt ttttgataag aaatacatga aaaacaaaat acaagtaaat
     9601 ttgtaaaaac tcttttacag ttttgataaa attaggagcg cattttttca ttcaactttg
     9661 attttctgaa agtaaaatat tatttttgtt tgattttgca atgaatttta ataaacaatt
     9721 taatcttaaa ttagttatgg cattacaagt tggagataaa attcctgatt atttgggagt
     9781 tgatcaaaac gaaaaaaaaa tttatagctc aaattttaaa ggaaaaaaat tgatattgta
     9841 tttttatcca aaggataata cctctggctg tacgtcggaa gcctgtagtt ttcgggataa
     9901 ttatgccgag ttgcgtcgca gaaattttga aataattggt gtgagtacag atgatattct
     9961 ttcccatcaa aaattcattc tcaaaaacaa tcttcctttt acgttaatta ctgataccga
    10021 tcatcggttg ggtgaagtgt ttggaacttt taaagagaaa aaacgttttg gaaggaaata
    10081 ttttggaatt gagagaacta cttttattat tgatgaaaat ggtatcataa cgcatattat
    10141 tcctcaggaa gaaattgata ccaaaaaaca tgctgaacaa attcttgaaa gggtagggga
    10201 tgaagaaaag tgaagaaagg cactttttaa aatcaaacat ttttcttaat aaaaaatttg
    10261 gaaggttatt ccattttttg aaataacctt tttgcgaatg gtttcatgta acatctttga
    10321 acattgagta caggtaagtg ttaagtaccg atttggcaat taaatttaaa atattatttt
    10381 ttattttttc cactgtttga gattcatcgc aagtttgctg taatgaattc aaacttactt
    10441 cattaagtcg tgattgcaag agcgactcaa tttcaaagca cgacatgttt ttgtttttta
    10501 accgaacaag ccacttttca atatcaggct ggaattgagt aattaccttt tgggcatctt
    10561 cttttatagc atccctgtaa atttctggaa gtaaatgagt cattgaattt acttcacgct
    10621 taaatcgaag attgatttca ttaaattcga tgtt
//
LOCUS       DAOSAS010000054        10616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4126_length_10616_cov_13.461225, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000054 DAOSAS010000000
VERSION     DAOSAS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10616
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4126_length_10616_cov_13.461225"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /gene="dnaK"
                     /locus_tag="PLR72_03820"
     CDS             complement(<1..1367)
                     /gene="dnaK"
                     /locus_tag="PLR72_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOK36369.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNQPVVIPNNEGKRTTPSIVAFLE
                     NGERKVGDPAKRQAIINPTRTVSSIKRFMGRTYDEVTAEAARVPFKVVRGENNLPKVD
                     IDGHLYSPQEISAIILQKMKQTAEDYLGTQVTDAIITVPAYFNDAQRKATKEAGEIAG
                     LNVRRIINEPTASALAYGLDKTNREMKIVVFDCGGGTHDVSILELGDGVFEVKATDGD
                     THLGGDDFDHRIIEWLVGEFKSENSGVDLTKDPMALQRLKEAAEKAKIELSTATSTEI
                     NLPYIMPVDGIPRHLVRTLTRAKFEQLIDDLVQRTIEPCRSALQKSGLTVNDINEVIL
                     VGGSTRIPAIQQAVEKFFGKAPSKGVNPDEVVAIGAAIQGGVLAGEVKDILLLDVTPL
                     SLGIETLGGVMTKLIEANTTIPTRKSEIFTTAADNQPSVEIHVLQGERPLASQNKSLG
                     RFHLDG"
     gene            1717..3543
                     /locus_tag="PLR72_03825"
     CDS             1717..3543
                     /locus_tag="PLR72_03825"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOK36370.1"
                     /translation="MIATETKIVNSEIIKEIEEKIENVYSYLNKQKEYVNSLNVFPVP
                     DGDTGLNMVLTIQGAIAALRSNNGYSSMTPGKFLKNFAENMLLNSRGCSGVILSLFAQ
                     GFTQITENNDFSKENIYKAIENGYRNAYEGTENPKEGTMLTIMRALKEKYFEFMQTEN
                     DPVAIIHDTLPYLREVLDKTPEMLPILKQAGVVDSGAAGFIILLEGINKELNHHNFNI
                     ISVPILLNLGRTTKKLLNKRISKFKKSSYFPLILNIDTSKIHNLRLREVFQNARNLLV
                     NNFHKNSKNTLQEQIINDLEEIDESWNPDIKYKFCTEFVLESNKISSKEELKNMLKDY
                     GNSLIIVNSENKYKVHIHTNKPNDIFSDMSKFGELLFTKVDDMKKQHRNFISDDIIDY
                     EKDKSIFCVVSGKGFAEILQNIGADDILCYGKNKPSVNQLVKCLNNLKAKNIIVAADD
                     SDILMALKYAVTLCKSNVLIVESDNPISLISMMVNISKDYDVHTIFDTAMNSLHNIRF
                     CAIAKSTRDIIVEGGTSVKKNDFFAVYKKKIVLANKNLEPLIVEIIQQLANDSSLITI
                     YKGIRAKKEENITSKLKEQFPSLEFEEYFGGQYKYDYYVTFE"
     gene            3556..4443
                     /locus_tag="PLR72_03830"
     CDS             3556..4443
                     /locus_tag="PLR72_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOK36371.1"
                     /translation="MKKKILIIVGDNLGLKRDQIDYEDLEIVKFPTIVDGKEYRENEE
                     YNASWLISKYVNENVVAMSSTLPQKDIVEIIEKNHSKYDLIIHVVMSSGLSAASFKVA
                     ENIRKQYENIIPIINVDSRQPINGVGNVLLGIIDMIKENNDLSIEEITKLCQQVVERT
                     YTYFVFPDLNYLYKGGRIGKAQSLMGSVLHIIPIVGAMGDNEEGIILPCGKGRTFKQV
                     NLQVMGLIKEKMKEKSVDKIKRAIVVYGYGDNNQDAIADFVEKAKSLPCEKFIIGKPG
                     LVDAVYCGPGAYMISVYLK"
     gene            4461..5078
                     /locus_tag="PLR72_03835"
     CDS             4461..5078
                     /locus_tag="PLR72_03835"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOK36372.1"
                     /translation="MMNTCFSYFLYLIIGYLIGSILFALLITKLATGQNIMELGNHNP
                     GAYNVFRNVGKGWGVLTGILDAVKALVPMLIAKYALNIQDEIALGCLGIGALLGHGYP
                     LYYHFEGGRAASILMGMLIFFVPYEFLFSFLIGGAIVLLFIKKEYGIWGPTLIIAFSA
                     ISCLFFPHTIEVKIIIWIGALITLFFNRDAVQERTKKLSPAVKKK"
     gene            5161..5631
                     /gene="folK"
                     /locus_tag="PLR72_03840"
     CDS             5161..5631
                     /gene="folK"
                     /locus_tag="PLR72_03840"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOK36373.1"
                     /translation="MSLVYLSLGSNLGDKTLHLNSAIEKLNQLAGTVVRQSSFYVTKP
                     WGFESKNDFLNSVVLLETFLSPFELLGKIQQIEQMMGRVSKTINGDYSDRIIDIDILL
                     YDNLVMEHPSLTLPHPLMTSRDFVLIPLFEIAPNITDPRTGKKIRNFLSTKSSN"
     gene            complement(5708..>6143)
                     /locus_tag="PLR72_03845"
     CDS             complement(5708..>6143)
                     /locus_tag="PLR72_03845"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOK36374.1"
                     /translation="REKELTELFIGKTTTESLSQVVYLTPAKAMKNEILFRFSTLKGV
                     VPSDDLSGIPYYITISPTTFPVTPATKEKKQKTINKQQKLNTILPASTLVNIGDGKNI
                     YFSQQFLIPQFGVVVPLPNEVLQNPKNKIYIDPQTGRLLKVE"
     assembly_gap    6144..6243
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6244..8453)
                     /gene="topA"
                     /locus_tag="PLR72_03850"
     CDS             complement(<6244..8453)
                     /gene="topA"
                     /locus_tag="PLR72_03850"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOK36375.1"
                     /translation="MPKNIVIVESPAKAKTIEKFLGKDYQVMSSYGHIRDLSEKNIGI
                     DFENNYQPLYEISPEKKKIVAELKKSIKDADMVWLASDEDREGEAIAWHLFEVLNLKE
                     EKTRRIVFHEITKDAILHAIEHPRPIDLHLVDAQQARRVLDRIVGFELSPLLWRKIRP
                     SLSAGRVQSVAVRLIVEREREINQFTPESFYKITGFFSGKDKNGSLVELKAELQQRFH
                     RKEEALAFLQKCKIEEFFIENIVTKPAKKSPAPPFTTSTLQQEAARKLGFSVSQTMML
                     AQRLYESGKITYMRTDSVNLSELALNTSEVEIKKIAGPQYVKKRHFVTKTKGAQEAHE
                     AIRPTYMNVHEIEGTAQEKRLYDLIWKRTMASQMADAELEKTTITIAMKNSPYQFIAN
                     GEVILFDGFLKLYAESSDEENENGSTNILPTMKVGEGLSPSKIIAQQHFSQHPPRYTE
                     ASLVRKLEELGIGRPSTYAPIISTIQNRLYVEKKDRPAEKRDYNVLILNNNKIEDKIE
                     TEARGAEKSKLFPTDIGMVVNDFLMEYFPSILNYNFTANVEKEFDSIAAGKEKWTEVI
                     DKFYKKFHAAVEKTLTSKDRKTKERILGIDPETGKQVSVKFGKYGPFVQLGTGEDGEN
                     PTFASLPKNYSMELITLEEALELFKLPRNLGEYENQPLTVAIGRFGPYVRQGNSFYSI
                     PKNLDPLTITNEQAIEIINKKKNEEKNRTIATFGEKNEIQVLNGRYGPYIVYNKT"
     gene            9311..10507
                     /locus_tag="PLR72_03855"
     CDS             9311..10507
                     /locus_tag="PLR72_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36376.1"
                     /translation="MKLYKKKIFFFLFFICLFYSCEDDNEYRVDSEFLPYLTRFEIEA
                     GKRGKNFNFQRDGIIIEFAKLKESTAGLTYKEHPVRIQIDKKYWEDISSSAGADIMKE
                     NLLFHELGHGILGRKHLNTVLENGDWKSIMCGGEKIDNRSWNINYRGFRRNYYIDELF
                     DESTTPPFLLTSIFNIDTLAYRLVIEKNFNDAQHAGWTIKNTTTYETSIDNRRLKFQS
                     KIDSLYFVFAKTNVNIQSDFKYKLTLECQNCQSKDQYGIIFGTLPDSFSPQGKKSIEY
                     FTIDQEQQMFMGNSNWYSFYTQLFKPEIQINGQNKLEIVKSGQILYYFINERYTYCSE
                     IEASEDGFYFGFIVPPKGTMLVDNFLIYEKTPRDVSAAKIKGTTIPEFKVLSCSVENF
                     RPDMKK"
BASE COUNT         3537 a         1644 c         1757 g         3578 t
ORIGIN      
        1 ccatccaaat ggaaacgtcc taatgattta ttttgtgaag ccaatggtcg ttctccttgc
       61 agtacatgaa tctccacaga aggttgatta tcggctgcag tagtaaagat ttctgattta
      121 cgtgtaggaa tagttgtatt ggcttcgatt aattttgtca taactccacc caatgtttca
      181 atacctaatg aaagaggcgt aacatcgagc aacaatatat cttttacttc acctgccaat
      241 acaccacctt gaatagctgc accaatagca actacttcat ctggattgac gccttttgaa
      301 ggagctttgc caaagaattt ttctactgct tgttgaatag ctggaatacg tgtagaacct
      361 ccaaccagga tgacttcatt tatatcgttg acggtaagac ctgatttctg taatgcactt
      421 cgacatggct ctatggttct ttgaaccaaa tcatctatta attgttcaaa ttttgctcgt
      481 gtcaaagtac gtactaagtg acgaggaatt ccatccactg gcataatata aggtaaattg
      541 atttccgttg aagtagcagt agataattca atctttgctt tttcggcagc ttcttttaat
      601 cgctgcaaag ccattggatc ttttgttaaa tcaacaccgc tgttttcact tttaaattcc
      661 cctaccagcc attcaatgat ccgatgatcg aagtcatcac ctcccaaatg agtatctcca
      721 tctgttgcct ttacttcaaa aaccccatct cctaattcca aaatggatac atcatgagta
      781 cctccaccgc aatcaaacac cacaattttc atttcccgat tagttttatc taatccataa
      841 gccaaagcag aagcggtggg ttcattaata atacgacgta catttaatcc ggcaatctcc
      901 cctgcctctt tcgtagcttt acgttgtgca tcgttaaaat aagcaggaac agtaattata
      961 gcatctgtta cttgcgttcc taaataatct tcagcggttt gcttcatttt ctgaagaata
     1021 atagccgaaa tctcttgtgg agaatataaa tgtccatcaa tatccacttt tggtaaattg
     1081 ttttctcctc ttactacttt aaatggtacg cgtgctgcct cggctgttac ttcatcgtaa
     1141 gtacgcccca taaaacgctt gatagaagat actgttcttg taggattaat aatagcctga
     1201 cgttttgcag gatctcctac ttttcgttcc ccattttcta aaaaagcaac tattgaagga
     1261 gttgttcgtt tcccttcatt atttggtatc acaactggtt gattcccttc catcaccgct
     1321 acacaagaat tggtagttcc taaatcaatc ccaataattt ttcccataac tttatttttt
     1381 ataattgtta tttaatgttc tcttaatttt ttcattcttt tgcttgcaaa ggttgtgcca
     1441 cctaactaat tgtcattaat aattaaactt tttgagccaa aacttaaaaa aagaaaaagt
     1501 accaacaaaa aacactgaca aaaatgcagg aaatatgcta ttgtgccaga aaaggaatat
     1561 tttagcactt attttgataa ctgagtaaaa ataattcagc tacccatatc aaaatgaaag
     1621 gtcaattttt atttttttgt cttttaaaat aaaattataa ctttggagct acaaataaaa
     1681 ataaaaataa attaatacct atttaattta attgctatga ttgcgacaga aacaaaaatt
     1741 gttaattctg aaatcataaa agaaattgaa gaaaaaattg aaaatgttta ttcttattta
     1801 aataagcaaa aagagtatgt aaatagtttg aatgtttttc ctgtgccaga tggtgataca
     1861 gggctcaaca tggtattaac tattcaagga gctattgcag ctttaagaag taataatggt
     1921 tatagttcga tgacaccagg caaatttttg aaaaattttg ccgaaaatat gcttctcaat
     1981 agtcgtggct gttcaggagt aattttatca ttatttgctc aagggtttac tcagatcact
     2041 gaaaataacg atttttctaa agaaaatatc tataaggcta ttgaaaatgg ttaccgaaat
     2101 gcttatgaag gcactgaaaa tccaaaagaa gggacaatgc ttactattat gcgagctcta
     2161 aaagaaaaat attttgagtt catgcaaaca gaaaacgatc cagtcgccat tatccatgat
     2221 accctacctt atttacgaga agtgctggat aaaactccag aaatgctccc tattttaaaa
     2281 caagctggtg tcgttgattc aggtgctgcc ggttttatta ttttactcga aggaatcaac
     2341 aaagaattaa atcatcataa ttttaatatt atttcagtcc caattctatt aaatttaggc
     2401 agaacaacaa aaaaattatt aaacaaacga atttcaaaat ttaaaaaaag ttcatacttc
     2461 cctttgatat taaacattga taccagcaaa attcataact taagattaag agaagtattt
     2521 caaaatgcaa gaaacctttt agtaaacaat ttccacaaaa acagcaaaaa cacattacaa
     2581 gaacaaatta ttaatgattt ggaagaaatc gatgaatcat ggaatcctga tattaaatat
     2641 aaattctgta cagaatttgt tcttgaaagt aataaaattt cttcaaaaga agaattaaaa
     2701 aacatgttga aagattatgg aaatagcctt attattgtaa atagtgaaaa taaatataaa
     2761 gttcatattc ataccaacaa accaaatgat atcttttctg atatgtccaa atttggtgaa
     2821 ttgcttttta ctaaagtaga cgacatgaaa aaacaacatc gcaatttcat tagcgatgat
     2881 ataattgatt atgaaaaaga taagagtatt ttctgtgtcg tttcagggaa aggttttgct
     2941 gaaatccttc aaaatatcgg agcagatgac attttgtgtt atggtaaaaa taaaccttct
     3001 gttaatcaat tagtaaaatg tttaaacaat ctgaaagcaa aaaatattat tgtcgcagcc
     3061 gatgatagtg atattttgat ggcacttaaa tatgctgtaa ctttgtgtaa atcaaatgtt
     3121 ttgattgtag aatctgataa ccccatttct ttgatcagta tgatggtaaa catatcgaaa
     3181 gattatgatg ttcataccat ttttgatacg gcaatgaaca gtcttcacaa catacgtttt
     3241 tgtgccattg caaagtccac acgtgacata attgtagaag gaggaacgtc agtaaagaaa
     3301 aatgattttt ttgctgttta taaaaagaaa attgttctgg caaataaaaa tttagagcct
     3361 ctcattgttg aaataattca gcaattagcc aatgatagca gtttaattac gatttacaaa
     3421 gggattcggg ctaaaaaaga agagaatata acaagcaaac tcaaagagca atttccttcc
     3481 ttagaatttg aagaatattt tggaggccaa tataaatacg attattatgt aacatttgaa
     3541 taaattaaac agggtatgaa aaagaaaatt ttaattattg taggtgacaa cttggggtta
     3601 aaaagggatc agattgatta tgaagatctt gaaattgtaa aatttccaac gatagttgat
     3661 ggtaaagaat atcgagaaaa tgaagaatat aatgcaagtt ggctaatttc aaaatatgtg
     3721 aatgaaaatg tggtagcaat gtcctccact ttgcctcaaa aggatattgt agaaattatc
     3781 gaaaaaaatc attcaaaata tgatctcatc attcatgtgg tgatgagcag cggactttcg
     3841 gctgcttcct tcaaagtagc tgaaaatatc cgtaaacaat atgaaaatat tattccgatt
     3901 attaatgtag atagccgaca acctatcaat ggagtaggaa atgtattgct gggtattatt
     3961 gatatgataa aggaaaataa tgatttaagt atagaagaaa taacaaaatt gtgtcagcaa
     4021 gtagtggaaa gaacctatac ttacttcgta tttcctgatt taaattatct ttacaaaggc
     4081 ggaagaatag gcaaagccca atctttaatg ggaagtgtat tgcacattat tcctattgta
     4141 ggtgcaatgg gagataatga agaaggtata atattaccat gcggtaaagg aagaactttt
     4201 aagcaagtta acttgcaagt aatgggtctc ataaaggaaa aaatgaaaga aaaatcagta
     4261 gataaaatca aaagggctat tgttgtctat ggctatggcg acaacaatca agatgccatt
     4321 gctgattttg ttgaaaaggc aaaatcgttg ccctgcgaaa aatttattat aggaaaacct
     4381 ggcttagtag atgctgtcta ttgcggacca ggcgcttata tgatttcagt gtatcttaaa
     4441 taaaatttaa aaaaaattat atgatgaata cctgtttctc ttattttctt tatttgataa
     4501 taggttattt gattggttca attttattcg ctctccttat caccaaactc gctacaggtc
     4561 agaatatcat ggaacttggc aatcacaatc ctggagcata caatgtattc aggaatgtag
     4621 gaaaaggctg gggagtgctc acaggaattt tagatgctgt gaaagcactg gttcccatgt
     4681 taattgccaa atatgcctta aatatacaag atgaaattgc attagggtgt ttaggaattg
     4741 gagctttgtt aggtcatggt tatcctttat attatcattt tgaaggtgga cgtgcagctt
     4801 ctatcttgat gggaatgctt atattttttg ttccttatga atttttgttt tctttcttaa
     4861 ttggtggagc tattgttctg ctatttatta agaaagaata tggaatttgg ggaccaacac
     4921 tgatcattgc tttcagtgcg atatcttgtt tgttctttcc tcatactatt gaagtaaaaa
     4981 ttattatctg gattggtgca ttaattacat tattttttaa tcgtgatgcg gtccaggaac
     5041 gaacaaaaaa gctgagtcct gccgttaaga aaaaatagaa atccacataa atttattact
     5101 gtccaacagt cagacttttc ggattttttt cagaattgtg actttatatt tttgcaaatt
     5161 atgagtcttg tttatttatc acttggaagc aatcttggag acaaaacttt acatttaaat
     5221 agtgcgattg aaaagctgaa tcaattggca ggaacagttg tccggcaatc ttcgttttat
     5281 gttacgaagc cgtggggatt tgaatcgaaa aatgattttt taaatagtgt agttttatta
     5341 gaaacttttc tttcaccttt tgagctcctg ggcaaaattc aacaaataga acaaatgatg
     5401 gggcgagttt ctaaaactat aaatggcgat tattcggata ggataattga tatagatatt
     5461 ttgctttatg ataatcttgt aatggaacat ccttctttga ctttgcctca tccacttatg
     5521 acaagccgag attttgttct tattccgcta tttgaaattg cacctaatat aaccgatcct
     5581 cggacaggaa agaaaataag aaatttttta tcaacaaaat cttccaatta aaaagcaatt
     5641 tgttatagac aaagaaaatt tttctaatag attccaaaat ttgttttagg ttgataaaat
     5701 aaaaacttta ttctactttt aacaatcgtc ctgtttgtgg atcgatataa attttgtttt
     5761 ttggattttg aagtacttca ttgggaagag gaacgacaac accaaattgt ggaataagaa
     5821 attgctgaga aaaataaata tttttcccat ctccaatgtt taccaatgta gaagcaggta
     5881 aaattgtatt taatttttgt tgtttattta ttgttttctg ttttttttct ttggtcgctg
     5941 gagttactgg gaaagtagta ggagaaatag taatatagta aggtatgcct gataaatcgt
     6001 cagaagggac aacgcctttc aacgtagaga aacgaaaaag aatttcattt ttcattgcct
     6061 tagcaggtgt taagtataca acctggctca atgattctgt ggtagttttc ccaataaaaa
     6121 gttctgttaa ttctttttct ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnngttttat tatatactat atagggtccg tatcgtccat ttaagacctg aatttcattc
     6301 ttttctccaa aagtagcaat agttctattt ttttcttcat tttttttctt attaatgatt
     6361 tctattgcct gctcatttgt aatagtaagt ggatccagat ttttaggtat ggaataaaaa
     6421 gaatttcctt gtcgaacata gggtccaaaa cgcccaatcg ccacagttaa aggttgattt
     6481 tcatattctc ccaggttacg cggaagttta aataattcca gggcttcttc gagtgtaatg
     6541 agttccatcg aataattttt cggcagggaa gcaaaagtag gattttcccc atcttctcca
     6601 gtaccaagtt gaacgaaggg tccatatttc ccaaatttta cggaaacttg cttgcctgtt
     6661 tcaggatcaa ttcccaaaat acgctctttt gtttttcgat ctttactagt taatgttttt
     6721 tcaacggctg catggaattt tttataaaat ttgtcaatta cttcagtcca tttttctttt
     6781 cctgcagcaa tactatcaaa ttctttttca acatttgcag tgaaattata attcaaaata
     6841 gaaggaaaat attccattaa aaaatcgttt accaccattc caatatcggt aggaaaaagt
     6901 ttagattttt cagcacccct tgcctcggtt tctattttat cttcaatttt gttattatta
     6961 agaatcagca cattataatc tcttttttca gcagggcgat cctttttctc tacatacaat
     7021 cgattttgaa tggtagaaat gataggagcg taagtggaag gacgcccaat tcctaattct
     7081 tccagttttc gcactaaact ggcttcggta tatcgaggag gatgctgtga aaaatgttgc
     7141 tgagcaataa ttttggaagg agataaacct tcacctactt tcattgttgg aagtatatta
     7201 gtgcttccat tttcattttc ttcatcactg gattctgcat acaatttcaa aaaaccatca
     7261 aataaaatta cttctccgtt agctataaat tgataaggtg aatttttcat ggcaatggtg
     7321 atggttgttt tttctaattc tgcatcagcc atttgtgaag ccatggttcg tttccaaatc
     7381 agatcataca aacgtttttc ttgagctgtt ccttcaattt catgtacatt catgtaagta
     7441 ggacgaatag cttcgtgggc ttcctgagct cccttggttt tggttacaaa atgtcttttt
     7501 tttacatatt gaggtccagc aatttttttg atttctactt cggaagtatt caaagccagt
     7561 tcagataaat tgacagaatc tgtacgcatg tatgtaattt ttccggattc gtacaagcgt
     7621 tgagccaaca tcatggtttg tgagacagag aaaccaagtt tccttgctgc ttcttgctgc
     7681 aaggttgaag tagtaaaggg aggagctggc gatttttttg ctggcttggt aacgatattt
     7741 tcaatgaaaa attcttctat tttacacttt tgtagaaaag ccaaagcttc ttcttttctg
     7801 tgaaaacgtt gctgtaattc tgcttttaat tcaacgagag aaccattttt atcttttcca
     7861 ctgaaaaacc cagtgatttt ataaaaagat tcgggagtga attgattgat ttccctttcg
     7921 cgttccacaa tgagacgtac agctacggat tgaactctgc cggctgaaag ggaaggacgg
     7981 attttacgcc ataatagtgg cgacaattca aatcctacaa tcctgtccaa cacacgacga
     8041 gcttgttgag catcaaccag atgcaaatct attgggcgag gatgttcaat ggcatgaaga
     8101 atggcatctt ttgtaatttc gtgaaaaaca atccgtcgtg ttttttcttc tttcaggttt
     8161 aatacttcaa acaaatgcca ggcaatggct tctccttcac gatcttcatc ggaagcgagc
     8221 caaaccatat cggcatcttt tatggacttt ttcagttctg ctactatttt tttcttttca
     8281 ggtgaaattt cgtaaagtgg ttgataattg ttttcaaaat caattccgat atttttttca
     8341 cttaaatcgc gaatatgtcc gtaactagac attacttgat agtcttttcc aagaaatttt
     8401 tcaatggttt tagctttggc tggagattct acaatgacaa tgtttttagg cataatgtgt
     8461 aagttatcaa atataattgc gtgcaaagta acaaaaattg ccaggtatca gccaaatttt
     8521 caactcatta ttttctcaat tttataataa acatatattt tcatccttta tccacctgat
     8581 aataagattt ataatcttct actggtacta aattgttatc aaaattatta aaattaaaat
     8641 attcatccaa aaataaaatt gctatattat gaaaattata cagaaaaaga aatatcatct
     8701 tttacttgag taaattcatt aaccgattga gacaaagaga aaaactatgc ttttaggaaa
     8761 tgtataatct tgtaaatcag tattttatag atcaatacaa attgaggtat ctctcaagac
     8821 aaggatttgc aagaaaaaaa ctcaacaata gctaccaaat taaacaatat ttcaatagaa
     8881 aactaaattt acaagcacaa aatacaaaca caatcccgta gtttcaacta aagatttaaa
     8941 tttttcaaac aaaatttctt acttgcggat tttaggtcat aaaaatttgt ttttttaatc
     9001 tgggtgtttt cctcaacccc actaacttga agcttgcccg gatttgtaat gtgggtgtac
     9061 cgaaggcaaa aaaataggat cacaaattgc aaatttatgt cagcagaaaa aattttaagt
     9121 taatcggcgt aaagtattcc gttattctat ggaaaatcct tgtgctctta tgcctcgcat
     9181 attgcgaact ccaaaccatt gattggtaat tgaatttctt tatagcataa taaattaaga
     9241 attagtttat agttttaaca aattatcgat gtttttattt atatttgtat aaagattagg
     9301 ttagaaaaaa atgaaactat ataagaaaaa aattttcttt tttcttttct ttatttgcct
     9361 tttttattcc tgtgaagacg acaatgaata tcgtgtggac agtgaatttt taccttactt
     9421 aacacgattt gaaatagaag caggtaaacg aggaaagaat tttaacttcc aaagggatgg
     9481 aataattatt gaatttgcca aattgaagga aagcacagca ggtttaactt ataaggaaca
     9541 tcctgttcgc atacagattg ataaaaaata ttgggaagat atttcttcct cagcaggagc
     9601 agatataatg aaagaaaatt tgctttttca tgaattggga catggcatat taggtcgaaa
     9661 acatttgaat actgttcttg aaaatggcga ttggaaatct attatgtgtg gtggagagaa
     9721 aatagacaat cgttcctgga atatcaatta tagaggcttc aggagaaatt attatattga
     9781 tgaattgttt gatgaaagta ctactccacc ttttctttta acttctattt ttaatattga
     9841 tactttagct tataggttag tgattgaaaa aaattttaat gatgcgcaac acgctggatg
     9901 gacgataaaa aatacaacta cttatgaaac ctccatagat aatagacgtt taaaatttca
     9961 atcaaaaatt gattcgctct attttgtttt tgccaaaact aacgtaaata ttcaaagtga
    10021 tttcaaatat aaactgactt tagaatgcca aaattgccag agtaaagatc aatatggaat
    10081 aatttttgga acccttccgg attctttttc accgcaagga aaaaaatcta ttgaatattt
    10141 tactattgat caagaacaac aaatgtttat ggggaatagt aactggtata gcttttatac
    10201 tcaattattc aaacctgaaa ttcaaattaa tggacaaaat aagttagaaa ttgttaaatc
    10261 agggcaaata ttatattatt ttatcaatga aaggtacaca tattgttctg aaatagaggc
    10321 aagcgaagat ggtttttatt ttggttttat agtaccgcca aaaggtacga tgttggtaga
    10381 taatttttta atttatgaaa aaacgccaag agatgtttct gcagcaaaga taaaaggaac
    10441 tacaattcct gaatttaaag ttttatcttg ttcagtagaa aatttcagac ctgatatgaa
    10501 aaaatgattt tcaattatct aaagtttaaa aatcggttgc acaatttttt ttaatgttga
    10561 gaaatggcta taattcaaga agaacattat ataaaaagaa aaactttcgt cgaagc
//
LOCUS       DAOSAS010000055        10609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4131_length_10609_cov_9.648664, whole genome shotgun sequence.
ACCESSION   DAOSAS010000055 DAOSAS010000000
VERSION     DAOSAS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10609
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4131_length_10609_cov_9.648664"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="PLR72_03860"
     CDS             <1..140
                     /locus_tag="PLR72_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOK36377.1"
                     /translation="NILPLATSMIPLSLKAYHEKQFENTAYFEPFYLKEFQTTHSKRK
                     L"
     gene            complement(233..1681)
                     /locus_tag="PLR72_03865"
     CDS             complement(233..1681)
                     /locus_tag="PLR72_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK36378.1"
                     /translation="MTTMKENNVKMTNYRWVICFMLFFATTINYLDRQVLSLTWKDFI
                     APEFHWTNNDYGTITALFSIFYAISMLFAGRLVDKLDTKKGFMWAIGVWSFGAILHAF
                     CGIATSGILTGHWLVSFGEAKQFISGVDNVAKIVNVSVTLFIFARIVLAIGESGNFPA
                     AIKATAEFFPKKDRAFATSIFNSGAQIGALIAPLTVPFIAKAWGWEMAFIVMGLLGFI
                     WMGFWVFVYKKPEVHPKINELELAYIQQDKDEEIEEVAPIEKKKVTFAQVFKYKQTWA
                     FAFGKFMTDGVWWFYLFWTPAYLSSVYGMDATESAVPIFILYLITMLSLVGGWLPTYF
                     VDKKNMHPYAGRMKAMLIFAFFPLLALLAQPLGTYSYWLPIIIIGIAGAAHQSWSANI
                     FSTVGDMFPKHAVATITGIGGMAGGIGSFLINKGSGALFDYSEAVGMRFMGFEGIDSG
                     YFIIFSICAVAYLIGWMVMKLLVPKYKPIVDL"
     gene            complement(1686..2354)
                     /locus_tag="PLR72_03870"
     CDS             complement(1686..2354)
                     /locus_tag="PLR72_03870"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOK36379.1"
                     /translation="MKFSKIQVLTKMAETGIVPVFYNSNIETAKNVVKACYEGGIRVF
                     EFTNRGEFAHEVFDEVVKYAEKECPEMIVGVGSVVDAPTAALYIQLGANFVVGPLFNP
                     DVAKVCNRRLILYVPGCGSVTEIGFAQEAGCDLCKVFPGEVLGPAFVKALKAPMPWSN
                     LMVTGGVKPEENNLKSWFDAGVMCVGMGSNLFPAEMIKANNWQGITNLCITSLSIIQG
                     LKKS"
     gene            complement(2382..3425)
                     /locus_tag="PLR72_03875"
     CDS             complement(2382..3425)
                     /locus_tag="PLR72_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOK36380.1"
                     /translation="MSKKIVTFGEIMLRLATPGYERFCQASQLIATFGGGEANVAVSL
                     ANYGMQTEFVTRLPQNDIAESCIMELRKHNVGTNYIVRGGERMGIYFLETGAVARGSK
                     VIYDRSHSAIAEIQPGMIEWREVLKDADWFHWTGITPAISQGAADTCLEAIQVANQMG
                     ITVSVDLNYRKNLWKYGKSASEVMPALVQGCDIILGNEEDAEKVFEIKPEGFDVTKTG
                     GDIEGAKFESVCKQLMEKFPRAKKVIITLRGSINANHNTWGGVLYSNGKLYQSRRYDI
                     THIVDRVGAGDSFMGGLIYGLLNYPNDDQHALEFAVAASCLKHTIYGDFNLVSVAEVE
                     NLMKGDVSGRVAR"
     gene            complement(3529..4935)
                     /gene="uxaC"
                     /locus_tag="PLR72_03880"
     CDS             complement(3529..4935)
                     /gene="uxaC"
                     /locus_tag="PLR72_03880"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOK36381.1"
                     /translation="MKKFLDENFLLQNETAQQLYHKHAEKLPIIDYHCHLIPAMVAED
                     YQFKTLTEIWLGGDHYKWRAMRTNGVDEKYCTGKDTSDWEKFEKWAETVPYTVRNPLY
                     HWTHLELKRAFGIEKLLSPKTAREIYEECTAKLQTKEYSAKNLMRKFNVEVVCTTDDP
                     VDSLEYHAKVKKEGFEIKMLPAWRPDKAMAVENPTVFRAYIEKLGQTADVNIVVFDDL
                     ITALRKRHDFFSEMGCKLSDHGIEEFYAEDYTENEIKTIFNKVYSGKELTKEEILKFK
                     SAMMVIFAELDWEKGWVQQFHYGAIRNNNTRLFKLLGPDTGFDSIGTFTTAKAMSKFF
                     DRLDVQNKLTKTIIYNLNPADNEVVATMIGNFQDGSVPGKMQFGAAWWFLDQKDGIEK
                     QLNTLSVLGLLSRFVGMLTDSRSFLSYPRHEYFRRILCNLLGNDIENGLLPYQELDFI
                     GQMVENISYYNAKSYFNF"
     gene            5225..6319
                     /locus_tag="PLR72_03885"
     CDS             5225..6319
                     /locus_tag="PLR72_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK36382.1"
                     /translation="MDYSSKKIRIKDIAKFSGVSKGTVDRVLHNRKGVSEKSRQAVEK
                     VLNEMNYSPNVFARSLASKKLYRIFCLFPAYQKGDYWENIENGFEQAAADFENYNVEI
                     LIEYFNQFDSSSFTKVSQSILEKEPDAIIMAPVFRNETIQFVNQLAEQKILFSYIDSM
                     IEETNFLTYYGQHSFQSGYVAAKLLFQTLPENSTVIITRTKRRGGAVSNQTTNRYNGF
                     MKYCEEFQLEKKFNLLKIEIEDDNEKANFQLFKNIFSQYSNVSAAITFNSKVYRLANY
                     LSLLNKTDIIILGYDVLEKNVTFLKQGIISYLIAQRPEKQAYFSIRDMCCELIFKKPI
                     NKINYVPIDILIKENVDYYINFSKYEASLF"
     gene            6371..7618
                     /locus_tag="PLR72_03890"
     CDS             6371..7618
                     /locus_tag="PLR72_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOK36383.1"
                     /translation="MKLEKYSIGIGDRFGHQGIAQLRAVIKAQQSGLNITPVWNKSNR
                     EHIYVQSHPEEVRKEADAAVKFLGFKGDYFVDADHVNLNTVEPFIASSDFFTLDVASY
                     IGKSTSNENINAFINSCKNFNKELSIPGIEEKFEISNEWLENIAKKFLLASEQAAVLY
                     QFIKEKKKNNPFIVEVSMDEVPTPQTPLELFFILKMLADKKIPLQTIAPKFTGRFNKG
                     IDYQGDIKQFAKEFEEDILVIDYAIKMFNLPQDLKLSIHSGSDKFSIYPFIAEITKKH
                     NKGFHLKTAGTTWLEEIIGLAAAGNEGLILAKKIYAIAFQRKNELCEPYSDVIDIDEN
                     KLPRPEEVETWTSEKFVNTLRHVPDHPDYNPNFRQLMHVAYKIAAEMKNEYFNLLEKY
                     SKIIETCVEENIFDRHLKRLFDL"
     gene            8172..10283
                     /locus_tag="PLR72_03895"
     CDS             8172..10283
                     /locus_tag="PLR72_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOK36384.1"
                     /translation="MKEKVKTYKNHFFALFILMIIGFNACTPSKKGITILNSGTDSYT
                     SLQISNPFGRAVTFIGNENVGAIGYELDGKTFWVKGYPEITVLVDSTLNYCWKQEERQ
                     VIMNVKEKEKETRFKFYLDSEKKPSAWHISLAASDGEYFTGIAERVVDGPQEESWKEG
                     ITTALNLRGERMEVKLKPTVSAYAPFYLSSENYAIFIHGTWPGVIDFCHKKPDRVQIS
                     FEGAQLSFSLMMDKSPADLVKRHALESGPSIIPPRWAFGPWRWRDEHINNKNYFDGSP
                     VKTPYNSDVVEDILMMQAYDIPCTAYWIDRPWGPGPTGFDDYKIDEQRLPKFEEMIHW
                     LNSKNIELMMWIAPFVMGEMADVAEERGYYLISKERENAHQVLIDFTNPEACKWWGEN
                     GPTKLAKMGIRGFKLDRGDGEKLLDSIQLVTSSGITYRENYNDYPRQYVKATYDAVKP
                     VLGDNFILFPRAQYTGSSRYGTMWAGDTDNSQEGLRSVIIGMQRCAVMGYPLWCSDTG
                     GYPKKLQREITQRWLGFSCFTPLMEVGPTNNHGFWGMNYEPSFDKELLATWRFYTKLR
                     MSLVDYLYHYAKVSNKTAMPVARPLFLEYPKQKESWKEWTTYKLGDDLLVSPIWEKGK
                     EQKLVYLPADETWIDLWNHKEYKGGKYIEVDAPTYKIPVFLKKGSSLQLPDLKSLYEE
                     SVKKTSIRYKMSDLEAKEGWK"
BASE COUNT         3676 a         1861 c         1657 g         3415 t
ORIGIN      
        1 aaaacatttt accacttgcg acctctatga tacccctttc tctgaaagct tatcatgaaa
       61 agcaatttga aaatactgca tattttgaac ctttttacct caaagaattt caaaccactc
      121 attcaaagag aaaattataa actaattctc ataatatttt tttaatgaaa aacatatgaa
      181 gttttaaaaa agaaagggaa gcaaaatttt actccccttt cattttaata aattataggt
      241 caacaatagg cttatatttg ggtactaata atttcattac catccagcct attaaataag
      301 ctacagcaca aatcgaaaaa ataataaaat atcctgaatc tattccctca aaacccataa
      361 atctcatacc tacagcttca gaataatcaa ataaagcacc tgatccctta tttatcaaga
      421 aggaaccaat accacctgcc ataccgccaa taccagtaat agtagcgaca gcatgttttg
      481 ggaacatatc tccaacagtg gaaaatatgt ttgctgacca cgattgatgg gcagcacctg
      541 ctatacctat aatgataata gggagccaat aagaataagt tcctaagggt tgagctaaaa
      601 gtgctaacaa cgggaaaaaa gcaaaaataa gcatagcctt catacgtcca gcataaggat
      661 gcatattctt cttatccaca aaatatgttg gcagccatcc tccaaccaat gaaagcatgg
      721 taattaaata caatataaaa ataggaacag cactttcagt ggcatccatt ccataaacgg
      781 aacttaaata agcaggtgtc cagaaaagat aaaaccacca tacaccatct gtcataaact
      841 tgccaaaagc aaaagcccag gtttgcttat atttaaagac ctgagcaaaa gttacttttt
      901 tcttttctat aggggcaacc tcttctattt cctcatcctt gtcctgttga atatatgcca
      961 attctaattc atttattttg ggatgaacct ccggtttctt ataaacaaaa acccaaaatc
     1021 ccatccaaat aaaacccagt aaccccataa cgataaaagc catttcccaa ccccatgctt
     1081 ttgctataaa aggaacagtt aaaggagcaa ttaaagcacc aatttgagct cctgaattaa
     1141 aaatgctggt agcaaatgct ctatcttttt tagggaaaaa ttctgctgtt gccttaattg
     1201 ccgcagggaa atttcctgat tcgccaattg ccagaacgat tcgtgcgaaa ataaataagg
     1261 taacactaac atttacaatt ttcgccacat tatctactcc gctaataaat tgcttggctt
     1321 ccccaaaact tacaagccaa tgacccgtca gaattcctga agtagcgatt ccacaaaaag
     1381 catgtaaaat tgctccaaaa gaccacacgc caattgccca cataaaacct tttttggtat
     1441 ctaatttatc taccaaacgt ccagcaaata acatacttat tgcataaaat attgaaaaaa
     1501 gagctgttat tgttccataa tcattatttg tccaatgaaa ttcgggagca ataaaatctt
     1561 tccatgttaa cgacaaaacc tgacgatcca gatagttaat ggtggttgca aaaaatagca
     1621 taaaacaaat tacccatcgg taattggtca tttttacatt attttctttc atggtagtca
     1681 tatttttatg atttttttaa tccttgaatg atagaaagtg aagtaataca taaattagta
     1741 attccttgcc agttgttagc ttttatcatt tcagcaggaa atagattaga acccattcct
     1801 acacacatca caccggcatc aaaccaactt ttcagattat tttcttctgg cttgacaccc
     1861 cctgttacca ttaaatttga ccatggcatg ggggctttca atgcttttac aaaagcgggt
     1921 ccgagaactt caccaggaaa tactttacac aaatcacatc cggcttcttg agcaaatcct
     1981 atttcggtta ccgacccaca gccagggaca taaagaatta aacggcgatt gcaaactttt
     2041 gcaacatctg gattgaacaa aggtcctact acaaaattgg ctcccaactg aatgtataac
     2101 gcagcagttg gagcatcaac cacagaacca acacccacta tcatttctgg acattctttt
     2161 tcagcatatt tcactacctc atcaaaaact tcatgagcaa attcaccgcg gttggtaaat
     2221 tcaaaaactc gaattcctcc ttcataacaa gcctttacca cattttttgc ggtttcaata
     2281 ttgctattat aaaaaactgg aactattcca gtttcagcca tttttgttaa aacttgaatt
     2341 tttgaaaatt tcatttttta tcgttatttt taaattcata attatcttgc tactcttcct
     2401 gaaacatcgc ctttcatcaa attttctact tctgctactg agaccaaatt aaaatcacca
     2461 taaatagtgt gttttaaaca agaagcagcc acagcaaatt caagagcatg ttggtcatca
     2521 tttggataat taagtaaacc ataaataagt cctcccataa atgaatcacc tgctccaacg
     2581 cgatctacaa tgtgtgtaat atcataacgt ctagattgat aaagttttcc attactatac
     2641 aaaacaccac cccaagtatt atgattggca ttgatagagc cccgtaaagt aataattact
     2701 ttttttgctc gtggaaattt ttccattaat tgtttgcata ccgattcaaa ttttgctcct
     2761 tcaatatcac cacctgtttt ggttacatca aaaccttctg gcttaatttc aaaaactttt
     2821 tctgcatctt cttcattccc taaaataata tcgcaacctt gaactaaagc gggcataact
     2881 tctgaggcag attttccata cttccagaga ttttttcgat aattgagatc caccgaaacg
     2941 gtaattccca tttgattggc tacttgaatc gcttcgagac aggtatcggc agctccttgt
     3001 gaaatggcag gcgttattcc tgtccaatga aaccaatcag catctttcaa tacttcccgc
     3061 cactcaatca tacctggctg aatttcagca atagcagaat gggaacgatc ataaattacc
     3121 ttactaccac gagctactgc tcctgtttcc aaaaaataaa ttcccattct ctcgcctcca
     3181 cgaacaatat agtttgtccc tacattgtgt tttctcaact ccataataca actttcagca
     3241 atatcatttt gaggtaaacg agttacaaat tcagtctgca taccataatt tgctaacgat
     3301 actgctacat tggcttctcc accaccgaaa gttgcaatta attgtgaagc ctgacaaaat
     3361 ctttcataac caggagtggc taatcgaagc attatttctc caaaagtgac aattttttta
     3421 ctcatctttt ctttagtttt ttaatagtta aatagtgaac gagtaaacaa aaataatatt
     3481 ttttattgtt atatagattt caattttttt agataatctt ttaatgtttc aaaagttgaa
     3541 ataagactta gcattataat aactaatatt ttcaaccatc tgaccaataa aatctaattc
     3601 ctgataaggt aataaaccat tttcaatatc attgcctaac aaattgcaca atatacgacg
     3661 aaaatattca tggcgtggat aggaaagaaa actgcgtgaa tcggtaagca ttccaacaaa
     3721 acgactcaat aaacctaaaa cagagagagt attcaactgc ttttcaattc cgtctttttg
     3781 atcgagaaac caccatgctg caccgaattg catttttcct gggacgctac catcctgaaa
     3841 attgccaatc atggtagcca caacttcatt gtcggcagga ttgagattat aaataattgt
     3901 tttagtaagc ttgttttgaa catcaaggcg gtcaaaaaac tttgacattg cttttgctgt
     3961 agtaaatgtt ccgatggaat caaaacccgt atcaggtccc aggagcttga ataatcttgt
     4021 attgttgtta cgtatggcac cataatgaaa ttgctgaacc caaccttttt cccaatcaag
     4081 ttcagcaaaa ataaccatca tggctgattt gaattttaaa atctcttctt ttgtcaattc
     4141 ttttccacta tatactttat tgaaaatagt cttgatttca ttttcagtat aatcttctgc
     4201 ataaaattct tcaatgccat gatcggaaag tttacaaccc atttcggaaa aaaaatcatg
     4261 ccgtttacgt aaagctgtaa ttaaatcatc aaatactaca atattgacat cagcagtttg
     4321 accaagtttt tcaatataag ctctgaaaac ggtaggattt tcaactgcca ttgctttatc
     4381 aggacgccag gcaggaagca tttttatttc aaacccttct tttttcactt tggcatgata
     4441 ttcaagtgaa tcgacaggat catcagtggt acatacaact tctacattaa atttacgcat
     4501 gagatttttt gcactgtatt ccttcgtttg cagtttagcg gtacattcct cataaatttc
     4561 tctggctgtt tttggcgaaa gtaatttttc aatgccgaat gctcttttca attcgagatg
     4621 tgtccagtga taaagcggat ttcgcacggt ataaggcact gtctcagccc atttttcaaa
     4681 tttttcccag tcacttgtat ctttgcctgt acaatatttt tcatccactc cattggtacg
     4741 cattgctctc catttataat gatcaccacc taaccaaatt tcagttaaag ttttgaattg
     4801 ataatcttca gctaccatag ctggaattaa atgacaatga taatcaataa ttggcaactt
     4861 ttcagcatgt ttatgataaa gctgctgagc cgtttcattt tgtaagagaa aattttcatc
     4921 taaaaacttt ttcatttttt attgatttta aaaaattatt ttaaacgttt attttctaat
     4981 aaaaaatttc tactgaaact tattttggat ataaaaataa taagcaatcc tttaaaaaat
     5041 gttataataa tgcttctatt tttcaacttt taagataaaa aacattaatt tttttaaata
     5101 aaaaacctat taaatttttg tgtccgtgca cgaaggtaca aaaaatttta tattctcaaa
     5161 aatttctatt atgtttgtaa aaattatttt tgtgtacgta cacgtattat aatttttaag
     5221 aaaaatggac tattcttcaa aaaaaattcg tattaaagat attgctaaat tttcaggggt
     5281 ttccaaagga actgttgatc gagttttaca taaccgaaag ggagtttctg aaaagagcag
     5341 acaggccgta gaaaaagtac tgaacgaaat gaattactcg cccaatgtat ttgctcgttc
     5401 attggcttcg aaaaaactct accgcatttt ttgccttttc cctgcttatc aaaaaggtga
     5461 ttattgggaa aatatagaaa atggttttga gcaagcagcc gctgattttg aaaactataa
     5521 tgtagaaatt ttgattgaat actttaatca atttgatagt tcttctttta cgaaagtttc
     5581 tcaatcaata cttgaaaaag aaccagatgc tattatcatg gctccggttt ttagaaatga
     5641 aacaattcaa tttgtcaatc aactggcaga gcaaaaaata cttttttctt acattgattc
     5701 aatgattgaa gaaaccaatt ttttaactta ctatggtcaa cattccttcc aaagcggtta
     5761 tgtagctgcc aaattgcttt ttcaaacgct gcctgagaat tcaacagtga ttattacgcg
     5821 aaccaaaaga cgtggtggcg cagtatcaaa tcaaacaacc aaccgctata acggatttat
     5881 gaaatattgt gaagaatttc aactggagaa aaaatttaat ttattaaaaa ttgaaataga
     5941 ggatgacaat gaaaaagcta attttcagct ttttaagaat attttttctc aatattctaa
     6001 tgtcagtgct gccattacct tcaactcaaa agtttataga cttgcaaatt acttatcatt
     6061 gttaaataaa acggatatta ttatacttgg ttatgatgtg ctcgaaaaaa atgtaacatt
     6121 tttgaaacaa ggtattatct cctatttaat agcacaacgc cctgaaaaac aagcatattt
     6181 cagtatccga gatatgtgtt gtgaattaat tttcaaaaaa ccaataaata agatcaatta
     6241 tgttcctatc gatattctga taaaagaaaa tgtcgattac tatatcaatt ttagtaaata
     6301 cgaagcaagc ttattttaaa ttgtttacat ttatatataa aattttgaat ttaaaaaaat
     6361 ataaattatt atgaaattag aaaagtatag tataggtatt ggtgatcgtt ttggtcatca
     6421 aggaattgct caattaagag ccgttatcaa agcccaacaa tcaggtttaa atattactcc
     6481 tgtttggaac aaatcgaatc gagaacacat ttacgttcaa tcacatcctg aagaggttcg
     6541 caaagaagct gatgctgctg ttaaattttt aggatttaaa ggagattatt ttgttgatgc
     6601 agatcatgtt aatttaaata cagtggaacc tttcatagct tcatctgatt tctttacatt
     6661 ggatgtagcc tcttatatag gtaaatctac ttcaaatgaa aatattaatg cctttattaa
     6721 ttcctgtaaa aattttaata aagaattatc aattcctggt attgaagaga agtttgaaat
     6781 aagtaatgaa tggctcgaaa atattgctaa aaaatttctt ttagcatcag agcaagccgc
     6841 cgtgctttat caatttataa aagaaaagaa aaagaataat ccatttattg tcgaagtttc
     6901 tatggatgaa gtgcccactc cacaaactcc actggaactc ttttttattc ttaaaatgct
     6961 ggctgacaag aagatacctc ttcaaaccat tgctccaaag tttactggta gatttaataa
     7021 agggattgat tatcaaggtg atataaaaca attcgcaaaa gaatttgaag aagatatatt
     7081 agttatagat tatgctataa aaatgtttaa tctgccacaa gatttaaaat tgagcataca
     7141 ttcagggagc gacaaatttt ctatttatcc ttttattgct gagataacta aaaaacataa
     7201 taaaggattt catttgaaaa cagccggtac tacctggttg gaagaaataa ttggtttagc
     7261 ggctgctggg aatgaaggtc tcatcctcgc aaaaaaaatc tatgctattg cttttcaaag
     7321 aaaaaacgaa ttgtgcgagc cttactcaga tgtgattgac atcgatgaaa ataaattacc
     7381 acgtcccgaa gaagttgaaa cttggacgag tgaaaagttc gtgaatacct tacgtcatgt
     7441 tcctgatcat cctgattata atcctaattt tcgccaatta atgcatgtag cttataaaat
     7501 agcagcagaa atgaaaaacg aatattttaa tttactcgaa aaatattcaa aaataattga
     7561 aacttgtgta gaagaaaata tttttgatcg gcatctaaaa cgtttgtttg atttataaat
     7621 tttctgtaat tatttcaaaa acctcttata aagaatatcc ttataacaat gatcctgtaa
     7681 ttgaaaaaga tgagttctct cttaaaaacc tgattttttc aaaaggaaga aaaaatgaga
     7741 ttttttgaag gaatattaaa ttttttattg ttttgatatt ttacaattat ttgccatata
     7801 taaaagttat ttttactaaa aaattagctt tttataaagc attaaatact ccttaaatag
     7861 tttggataat atatttcagt attcatgaag tttattccca ataaacgaga gtaagctcac
     7921 tttaagatta aaggtaagat attgaaatat aatgatacat acaaattttt caaaaaactt
     7981 tttcaggtgt tttttatact ttttataaca attaactatg aataaattaa tttcgagaaa
     8041 cttttcggag tggtctgggt attccagggt tcctatgtaa aaataacatc atattattcc
     8101 aaattaaatt taatcccagc atgaattgca gataatcatt aaaataacaa aaagatatta
     8161 aacctatcaa aatgaaagaa aaagtaaaaa cctataagaa tcattttttt gcattattta
     8221 tccttatgat aattggattt aatgcctgca ctccatcaaa aaagggaatt accattttga
     8281 attctggcac tgattcatat acctctctgc aaataagtaa tcctttcgga agagccgtta
     8341 cctttattgg caatgagaat gtaggagcta taggttatga gctagatggt aaaaccttct
     8401 gggtgaaagg ttatcctgaa attacagttt tagtagacag caccctgaat tattgctgga
     8461 aacaggagga aagacaagta ataatgaacg taaaggaaaa agaaaaagaa acacgattca
     8521 aattttattt agattcagag aaaaaaccgt ctgcctggca tatctcttta gctgcaagtg
     8581 atggcgaata ttttacggga attgcagaaa gagttgttga tggtccacag gaagaatcat
     8641 ggaaggaagg aataactacg gcactgaact tgcgtggaga aagaatggaa gtgaaattga
     8701 agccaacggt atcagcttac gcacctttct atttatcatc tgaaaattac gcaatattca
     8761 ttcatgggac gtggccaggt gttatcgatt tttgtcataa aaaaccagat cgagtgcaaa
     8821 tttcatttga gggggcacaa ttatctttct ctttaatgat ggataaatct ccagccgact
     8881 tagtaaaacg tcatgcattg gaatcaggac cttctattat tccaccccgt tgggcattcg
     8941 gaccgtggcg atggcgtgat gaacacataa acaacaaaaa ttattttgac ggttcaccag
     9001 taaaaacacc ttataactca gatgtagttg aagatatttt aatgatgcag gcttatgata
     9061 taccgtgtac tgcctactgg attgatcgcc cttggggacc cggtccaact gggtttgatg
     9121 attataaaat tgatgaacaa cgattgccta aatttgaaga aatgattcat tggctcaatt
     9181 ccaagaatat cgaattgatg atgtggattg cccctttcgt aatgggagaa atggctgacg
     9241 tggctgaaga aagaggatat tacctgataa gtaaagaaag ggaaaatgct catcaagtac
     9301 tcatagactt taccaatccc gaagcctgta aatggtgggg tgagaatgga cctacaaaat
     9361 tggcaaaaat gggtataaga ggctttaaac tggatcgtgg cgacggagag aaactccttg
     9421 attctattca attagtgaca tcatcaggaa ttacttatcg tgaaaactac aatgattatc
     9481 cacgtcaata tgtcaaagca acttacgatg ctgttaaacc agttttaggt gacaatttta
     9541 ttcttttccc tcgtgcccag tacacgggaa gcagtcggta tggaaccatg tgggcaggcg
     9601 atacggataa ttcacaggaa ggtttgcgtt ctgttatcat tggaatgcaa cgttgtgcag
     9661 taatgggtta tccgctttgg tgctctgata caggcggtta tcctaaaaag ttgcaacgcg
     9721 aaataacgca aagatggttg ggattttcct gcttcactcc acttatggaa gtgggaccca
     9781 ctaacaatca cggtttttgg ggaatgaatt atgaaccgtc ttttgacaag gagttacttg
     9841 ctacctggcg attttatact aaacttagaa tgagccttgt ggattatctt tatcattatg
     9901 ccaaagtttc caacaagaca gcaatgcctg ttgctcgtcc cttatttctt gaatatccta
     9961 aacaaaaaga aagttggaaa gagtggacaa cctataaatt aggagacgat ttgttggttt
    10021 cgccaatctg ggaaaaagga aaagaacaaa aattggttta tcttcctgct gatgaaacgt
    10081 ggatcgattt gtggaatcac aaagaatata aaggaggaaa atatattgaa gtggatgccc
    10141 ccacttataa aattcctgta tttctgaaaa aaggcagtag tttacaatta cctgatttaa
    10201 aatcccttta tgaagaatca gtaaagaaaa cttctattcg atacaaaatg agtgacttgg
    10261 aagcaaaaga aggatggaaa taaaaaaaca ttttatgatt agccccattt tatactaaat
    10321 tgttactaaa attattaacc caaatttctc gttaggtttc aactctctaa atcattatag
    10381 tggattaagt aaaaaacact aattttgaag cagagaaaaa gatttttaag tttactatta
    10441 taaagtttgt cttgttaatt ttcttttaaa aaatattgat attcaatgct ttataaatta
    10501 tgaaattatt aaaattattt ttttaatgta gactgatatt tttattttgt aatttaatta
    10561 gaaaaatctt taaaagtttt gttaatatca aaaatattaa atatatttg
//
LOCUS       DAOSAS010000056        10533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4169_length_10533_cov_13.487211, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000056 DAOSAS010000000
VERSION     DAOSAS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10533
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4169_length_10533_cov_13.487211"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2673)
                     /locus_tag="PLR72_03900"
     CDS             complement(<1..2673)
                     /locus_tag="PLR72_03900"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOK36385.1"
                     /translation="MKIRFTIHYVTQWGQTISVFILSDQNKNPQQFLMTCNKDFEWSI
                     EIEFKTPLKFIQYRYGLLHPDSKIEYEYGEYRYLNMDTSYSNINILDNWRVRFGDSPF
                     ETTAFLDCFFRRENQFIQPLKDKNANLLLQLRCAQIEPDRHFAIVGNQEVLGNWDVRK
                     KIRLDDSDFPIWKISLNASKFKFPLEYKYLIVDTETDEVLAWGGGPNRKITTISDKTL
                     NIVNDEHFIRTIPSWHGTGVSIPVFSLRSEESFGVGEFNDLKKMVDWAKMTGQRIIQI
                     LPVNDTIVYHTNYDSYPYNAVSVYALHPIYLHLENLGMLKDEKQNEYFAQKKKEFNAK
                     TFSDYQNVLNVKWEYFKMIYIQEKSKIFSSEDYQTFFETNKRWLMPYAAFSYLRDVYG
                     TPNFREWKNFSCFDLKEIENFSSPEQPHYDEIAFYYFLQYHLHKQLSDVHQYARANGI
                     AIKGDIPIGVSPYSVDVWTEPELFNTQVQAGAPPDDFSVIGQNWGFPTYNWEEMEKRN
                     YLWWKNRFQKLAEYFDAYRIDHILGFFRIWEIPNDAIWGLAGNFYPSLPFTREELESK
                     GLAWDENRFLKPYLKEHVLYATFGKYTEEILREYFLPDGWQTYKFKPEYDTQKKIEAH
                     FTSLGYNFSNKDVFIRDGLYALHCEVLFLRDLREPNKFHPRIGMHSTASFRDLPEETR
                     RLLDRLYVDYFYHRHTEFWKQQALKKLPELISATKMLACGEDLGMVPDCVPEVMQFLE
                     ILSLEVQRMPKKPNREFGNPAEAPYLSVCTTSTHDMNPLRAWWEEDPVITQRFYNHVL
                     RLPGEAPKTCETWIVERIIEQHLNSNAMWVILPWQDWIALDEKLRYEHPFAERINVPS
                     NPRNFWCYRMHLTLEQLLKEDDFNHYLKELITKSG"
     gene            complement(2823..2893)
                     /locus_tag="PLR72_03905"
     tRNA            complement(2823..2893)
                     /locus_tag="PLR72_03905"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2859..2861),aa:Cys,seq:gca)
     gene            complement(2973..3449)
                     /locus_tag="PLR72_03910"
     CDS             complement(2973..3449)
                     /locus_tag="PLR72_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36386.1"
                     /translation="MKMNIKYVIFFCLLFIGTTLQAEEARKNPSIKDMHEKKWSYLVK
                     KAQLTPQEAAIVKPIFMEYEQSLWQLHSQARDTFKKNYMKGENPQINYEELNELYINV
                     QLKQAELLKKYHHQLRKVLSAKTLFNYYQAEQSFKRQLLRHMPKQKNNKALKQPQE"
     gene            complement(3433..3777)
                     /locus_tag="PLR72_03915"
     CDS             complement(3433..3777)
                     /locus_tag="PLR72_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36387.1"
                     /translation="MKKQTHISLEEIGKKLPFKVPENYFEEFASQIDEQIAPKKPSWK
                     QISQPWLYLVALFVGILFMVQIFYTIYVKIEKKQKENYELYVLSQIDEPFIMNYYLTN
                     EENNLSGNENEY"
     gene            complement(3897..4445)
                     /locus_tag="PLR72_03920"
     CDS             complement(3897..4445)
                     /locus_tag="PLR72_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK36388.1"
                     /translation="METGEEELMFQLNQANTKAAAFSMVVRKYQERLYWHIRKMVLSH
                     DDANDILQNTFLKAWEGIDNFRGDSLISTWLYRIATNETLTFLANKRMQNITSMDELE
                     EVLAQNLEADKYFDGDEAQLKLQKAILTLPEKQRLVFNMKYFDDLTYEEMEAILDTSV
                     GALKASYHIAVKKIEKYLTEEE"
     gene            complement(4525..5481)
                     /locus_tag="PLR72_03925"
     CDS             complement(4525..5481)
                     /locus_tag="PLR72_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOK36389.1"
                     /translation="MRILVSTKLPLEGFGSLIEKFEVVFPESDIFSKEEVIQIIPSFD
                     AFISTFQFKVDKEVIDAGAAGRLKIISNYGVGYDNVDVEYAAKKGIVVTNTPDPVTEP
                     TAEHTIGLMLAAARRISECDRKLRIPNGLKWGLLENLGQSLYGKTLGIIGMGRIGKAV
                     ARRALAMDMKIIYYQRNRLDPTLEEQFKAEFIEQKTLLSESDVVTLHLPLNENTYHLM
                     NEETLRLMKSTAILINTARGPIVDEIALLKALKNHWIAAAALDVFENEPDITSGLLEL
                     DNVVLSPHNGTATLEARNEMSKFAAQNIINFFEGNGNITKVN"
     gene            5707..6951
                     /locus_tag="PLR72_03930"
     CDS             5707..6951
                     /locus_tag="PLR72_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOK36390.1"
                     /translation="MVKVKPFKGIRPPKDLVEKVASRPYDVLNSEEARVEAEGNPMSL
                     YHIIKPEIDFECGTDEHEEKVYLKAVENFAKFRENGWLQKDKTEHYYVYAQTMNGHTQ
                     YGIVLCAYIDDYLTGKIKKHELTRKDKEEDRMKHIRMTNANMEPVFLAYPFQPELDKI
                     VSEVIKNKPEYDFKAKDGIIHSFWIIDDKDKMKKITEIFDNIPSLYIADGHHRTAAAV
                     AVGNEKKQSNPSHTGNEEYNYFMAVCFPENQLRIMDYNRVVKDLNHLTDEEFLKKISE
                     YFLVEKKGKEIYQPNKLHNFSLYLAGNWYSLTAKEGTYNDADPIEGLDVTVLSHYILD
                     NILDIKDLRTDKRIDFVGGIRGLGELKKLVDSGKMKAAFALYPVSMKQLIAIADSGNI
                     MPPKTTWFEPKLRSGLVIHELE"
     gene            7002..8990
                     /locus_tag="PLR72_03935"
     CDS             7002..8990
                     /locus_tag="PLR72_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOK36391.1"
                     /translation="MRFRIFSVILILLAFLLLEGCNTASRIKKADNRFEIGEYYAAGE
                     LYKRCYSQLSSKDKPMRAYVAFQQGECYRLTNYYRAEQAFANSIRNNFSDSIVFLRYA
                     QQLHKNRKYADALKYYKIYLQKDSSNIVAKNGILAIDSIEQWKNTPTRYQINYEKQFN
                     ERKSSSFSPSFMNSSNDVLAFTSTRPMNKKAPPKADPITGLPKNNLLIVKKNAIGKWE
                     KPEFIEGEINTLDSDEGVCSFSSDGKTMYFSRAQQSQEKDMGAEIYYSKRAGGSWSTP
                     QRITIFKDSSISVAHPAIAPDGETLYFVSDHPSGFGGKDIYRAVLESGECKYVENLGP
                     SINTPGDEMFPTIRIDGTLYFSSNGHPGFGGLDIFKAVQNKDGMWQIKNMGMPVNSSS
                     DDFGITFEGNAERGYFSSNRNENRGYDAIWSFELPEIIYLLEGKVVDENGQPVPDAKI
                     RIVSNTGVNAYTQTKKDGSYRFKLEKDAEYVMLASARGYLNQQNKLSTMGVQESKTYH
                     VDFKLYPINKPIKIENIFYEFGKWDLTPASEKALQELVQVLKDNPNITIELSSHTDYV
                     GDNQFNKILSEKRAQSVVDYLIKHGIEKERLTAVGYGEEQPVVVDENLVQKYPFLKLG
                     QPLTESYILTLTPEQQEIANQINRRTEFKVLKTTYNLY"
     gene            9019..9813
                     /locus_tag="PLR72_03940"
     CDS             9019..9813
                     /locus_tag="PLR72_03940"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOK36392.1"
                     /translation="MKQYLHLLNKVLTEGVYKEDRTGVGTISIFGHQMRFLLEEGFPC
                     LTTKKLHLKSIIYELLWFLKGDTNVKYLQENGVTIWDEWADKNGDLGPIYGYQWRSWP
                     DYNGGFIDQISEAVNTIKNNPNSRRIVVSAWNVAQLPQMKLPPCHILFQFYVVNGTLS
                     LQLYQRSADIFLGVPFNIASYSLLLMMMAQVTGLKAGEFIHTLGDAHIYLNHLDQVKL
                     QLTRIPRPLPTMKINPDVKDIFSFQYSDFELINYDPYPPIKGEVAV"
     gene            9944..10447
                     /locus_tag="PLR72_03945"
     CDS             9944..10447
                     /locus_tag="PLR72_03945"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOK36393.1"
                     /translation="MPKISIIAAVADNYAIGNLNKLPWHLPADLKYFKQLTTGHPIIM
                     GKRTFDNLPNGPLPNRKNIVLTSVISEGITKGYVEANSLEEALEICKNDDEVFIIGGV
                     SVFKQAIDIADRMYITWVHENFKADAFFPEINFDEWEEICREDHPADDKNPYAYTFSV
                     YERKKRK"
BASE COUNT         3533 a         1705 c         1725 g         3570 t
ORIGIN      
        1 tcctgatttt gtaattaatt ctttaagata atgattaaag tcgtcttctt ttaataattg
       61 ttccaatgtc aaatgcattc gataacacca gaaattacgc ggattactag gtacattgat
      121 tcgttcggca aaaggatgtt cgtatcgaag tttttcatct aacgctatcc aatcttgcca
      181 cggaagaatt acccacatcg cattagagtt gagatgttgt tcaataattc tttcaacaat
      241 ccacgtctcg caagtttttg gtgcttcgcc aggtaatctg agcacatgat tatagaaacg
      301 ttgtgttatt actggatctt cttcccacca tgcccttaat ggattcatat cgtgagtgga
      361 agtagtacaa actgataaat aaggagcttc tgctggattt ccaaattctc tattcggttt
      421 tttgggcatt cgttgaactt ccaaactcag aatttctaaa aattgcatca cttcgggcac
      481 acaatcaggc accattccta aatcttcgcc acaagctaac attttagttg ctgaaattaa
      541 ttcgggcaat tttttcaaag cttgttgctt ccaaaattct gtatgtcgat gataaaaata
      601 atctacatat aatcgatcaa gcaaacgtcg agtttcttca ggtaaatcac gaaaagaagc
      661 agtgctatgc attccaatac gtgggtgaaa tttatttggt tcacgtaagt cacggagaaa
      721 aagaacttcg caatgcaatg cgtataaacc gtctcgaata aatacatctt tattgctaaa
      781 attataacct aaggaagtga aatgagcttc aatttttttc tgcgtatcgt attctggttt
      841 gaatttatag gtctgccaac catcaggtaa aaagtattcc cggagaattt cttcggtata
      901 ttttccaaaa gttgcataca aaacatgctc ttttaaataa ggttttagaa aacgattttc
      961 atcccaggcc agtccttttg attctaattc ttcacgtgta aaaggtaaag atggataaaa
     1021 attgcctgca agtccccata tagcatcatt tggaatttcc caaatacgaa aaaagcctaa
     1081 aatatgatct atacgataag catcaaaata ttctgcaagt ttttgaaaac gatttttcca
     1141 ccataaataa ttcctctttt ccatttcttc ccaattatag gtaggaaatc cccaattttg
     1201 gcctatcaca gaaaaatcat caggcggagc accagcttgt acttgggtgt tgaacaattc
     1261 aggttcagtc catacatcca cactataagg acttacccca ataggaatat cacctttaat
     1321 agctatcccg ttagcacgag catattgatg aacatcagag agttgcttgt gtaaatgata
     1381 ttgcagaaaa taataaaagg caatctcatc ataatgaggt tgttcaggtg atgaaaaatt
     1441 ttctatttct ttcaagtcga aacaagaaaa atttttccat tctctaaaat ttggtgtgcc
     1501 ataaacatct cttaaataag aaaatgctgc ataaggcatg agccatcttt tgttcgtttc
     1561 aaaaaaagtt tgataatcct ctgaagagaa aatttttgac ttttcctgaa tataaatcat
     1621 tttaaaatat tcccatttta cattcaatac attttggtaa tcagaaaaag tttttgcatt
     1681 gaattctttc tttttctgag caaaatattc attttgcttt tcatctttga gcatacctaa
     1741 gttttcgagg tgtaaataaa taggatgtaa ggcataaact gaaactgcat tataaggata
     1801 agaatcgtaa tttgtatgat acacaatagt atcattaact ggtaaaattt gaatgatacg
     1861 ttgacctgtc attttagccc aatcaaccat tttctttaaa tcgttaaact cccctactcc
     1921 aaaactttcc tcgctacgaa gtgaaaatac aggaatagac actcctgtac catgccacga
     1981 aggaattgtt cgaataaaat gttcatcatt aacaatattc aatgttttgt cggaaatagt
     2041 tgttattttt ctgtttggtc cgcctcccca tgcaagcact tcatctgttt cagtatcaac
     2101 aattaaatat ttatactcca gtggaaattt gaatttagaa gcattgagcg aaattttcca
     2161 aataggaaaa tctgaatcat ccaaacggat tttttttctt acatcccaat tccccaacac
     2221 ttcctgatta ccaacaatag caaaatgtcg atcaggttca atttgagcac aacgcaattg
     2281 caataataga tttgcatttt tatcttttaa tggctgaata aattgatttt ctcttctaaa
     2341 aaaacagtcc aaaaatgcag tggtttcaaa aggactatct ccaaaacgaa cacgccagtt
     2401 gtctaaaata ttgatatttg agtaagaagt atccatattc aaatatctgt attctccata
     2461 ttcatattca atttttgaat ctggatgcaa taaaccgtag cgatactgaa taaattttaa
     2521 aggagtttta aattctattt caatgctcca ttcaaaatcc ttattgcaag tcattaaaaa
     2581 ttgttgcgga tttttatttt gatctgaaag aataaataca gagatggttt gtccccactg
     2641 agtaacataa tgaattgtaa aacgtatttt cattatgaat ttaaaatgat acctttaaga
     2701 gtaatatttt tttgaaaaaa caaatatata aaattcccac cttgaattga taaaacaaga
     2761 agggaatttt aaaaaaataa aaaaaacctt ctcgaattgc aaacaatcta aagaaggttg
     2821 cgaggtactt ggcggattcg aaccgccgtt tacggttttg cagaccggtg cctaacctct
     2881 cggccaaagt accttatgct gggattgcaa agataatatt tttttctttt tcagcaaagt
     2941 tttgaaatgg ggaatgaaaa cgaggcaaaa gcttactctt ggggttgttt taatgcttta
     3001 ttattttttt gctttggcat atgtcgtaat aactgacgtt tgaaactctg ttctgcttgg
     3061 taataattaa aaagagtttt agcagagagt acttttcgta attgatgatg gtattttttt
     3121 aataattcag cttgttttaa ctgtacatta atgtaaagtt catttaattc ttcatagttg
     3181 atttgtgggt tttcgccttt catataattt ttcttgaaag tatcccgtgc ctgactatgg
     3241 agttgccaca acgactgttc atattccata aaaattggtt tcacaatagc cgcttcttga
     3301 ggagttaatt gagctttttt tactaaatag ctccattttt tttcatgcat gtctttaata
     3361 ctgggatttt tacgagcctc ttctgcttga agcgttgtcc caataaacaa taaacagaaa
     3421 aaaattacat atttaatatt cattttcatt ccctgataag ttattttctt cattggttaa
     3481 ataataattc attataaaag gttcatctat ttgagaaagt acgtataatt cgtaattttc
     3541 cttttgtttt ttttcaattt taacgtaaat ggtataaaaa atttgtacca taaataatat
     3601 ccctacaaac aaagctacca gataaagcca tggttgcgaa atttgtttcc aggaaggttt
     3661 tttaggtgca atttgttcat ctatttgtga agcaaattct tcaaaataat tttctggaac
     3721 tttaaatggt aattttttgc ctatttcttc aagggatata tgtgtttgtt ttttcatttt
     3781 tttatttttt gactaaaaaa aatggagtta gtttaatttt acatgcatat cgattgtttt
     3841 ttaacacatt tagctgagat ttgtttgatt tataaagtgt atagtcgaaa taattattat
     3901 tcttcttcag taagatattt ttctattttt tttaccgcaa tgtgataaga agcttttaaa
     3961 gctcctactg aagtatccaa aatagcttcc atttcttcat aggtgaggtc atcaaaatat
     4021 ttcatgttaa aaaccagtcg ttgtttttct ggcaatgtca aaatggcttt ctgaagtttt
     4081 agctgtgctt catcgccatc aaaatattta tctgcttcca gattttgtgc cagaacttct
     4141 tctaattcgt ccattgaagt gatattttgc attcttttat ttgctaaaaa agttaatgtt
     4201 tcattggttg caattctata aagccatgta gaaatcaacg aatcgcccct aaaattatct
     4261 attccttccc atgccttcaa aaaagtattt tgtaaaatat cattggcatc gtcatgagat
     4321 aaaaccattt ttcggatatg ccaatataaa cgttcttggt attttctgac aaccatagaa
     4381 aatgcagcag cctttgtatt agcctgattc aattgaaaca tcaactcttc ttcgcctgtt
     4441 tccatgttaa gttttgttga atggcttccg ttgttgtctt tattcttatt tttattcatt
     4501 ttgattgctc agaaatcaat tagtttaatt tactttggtt atattgccat tcccttcgaa
     4561 aaagttgata atattttgag ctgcaaattt actcatttca tttcgagctt ctaaagtagc
     4621 agtgccatta tgaggtgata aaacgacgtt gtctaattcc agcaatccag aagtaatatc
     4681 tggctcattt tcaaatacat ccagcgcggc cgctgcaatc caatgatttt tcagtgcttt
     4741 aagaagagct atttcatcaa caatcggtcc tcttgctgta ttgattaaaa tagcagtcga
     4801 tttcatcaat cgaagtgttt cttcattcat aagatgataa gtgttttcat ttaaaggaag
     4861 atgtaatgtt actacatcac tttcgcttaa caaagttttc tgttcaataa attctgcctt
     4921 aaattgctct tccaatgttg gatctaagcg atttctctgg taataaataa ttttcatatc
     4981 cattgcaaga gcacgtcgag caactgcttt gccaatacgt cccattccaa tgatccctaa
     5041 cgtttttcca tacaatgatt gtccaagatt ttccagtaat ccccatttta atccattagg
     5101 gattcttaat tttctgtcgc attcactaat tcttcgtgca gcagctaaca ttaaacctat
     5161 ggtatgttct gctgtaggtt ctgtgactgg atcgggtgta ttagttacta caattccctt
     5221 tttagcagca tattcgacat caacattgtc atatcctact ccataattgg atattatttt
     5281 tagccgtcct gcagcaccag catcaatcac ttctttatca actttgaact gaaaagtaga
     5341 aataaaagca tcaaaagaag gaattatttg aataacttct tctttactaa aaatatcgct
     5401 ttcaggaaaa accacttcga atttttcaat taaagaacca aaaccttcta aaggtaattt
     5461 agttgaaacg agtattcgca tatctctgaa ataataaatt taatcctcat agaatttaat
     5521 aaataagaaa aaacaaaagt aatgaaacaa caaaaagtgt acaattatat ttttcaaatt
     5581 ttgagttttt agcagacatt tgattaaaaa caattcaact aagcaagttt ttgtactttt
     5641 aaaaaattat atttttttat ttatctttgt aaacttattt gcgaattcaa atttaactga
     5701 ttaattatgg taaaagtaaa accatttaaa ggcattcgtc ctcctaaaga tttagtagaa
     5761 aaagtagctt ctcgacctta tgatgtgtta aattcagaag aagccagagt agaagctgag
     5821 ggcaatccta tgtcattata tcatatcatt aaaccagaaa tcgattttga atgtggaact
     5881 gatgaacatg aagaaaaagt gtatttaaag gcagtagaaa attttgccaa atttcgagaa
     5941 aatggttggt tgcagaaaga taaaacagaa cattattatg tatatgctca aacaatgaat
     6001 ggtcacacac aatatgggat agtgctgtgt gcatacattg atgactatct tactggaaaa
     6061 atcaaaaaac acgaattaac ccgcaaagat aaagaagaag accgtatgaa gcatattcgc
     6121 atgactaatg ccaatatgga accagttttt ctcgcttatc cttttcagcc tgagttagac
     6181 aaaattgtat ctgaggttat taaaaataaa cccgaatatg attttaaggc aaaagatgga
     6241 ataattcata gcttttggat aatcgatgat aaagacaaga tgaaaaaaat tacagaaatt
     6301 ttcgataata ttccttcact ttatattgcc gacggtcatc atcggacggc tgcagcagtt
     6361 gcagttggaa atgaaaaaaa acaatcgaat ccttctcata cagggaatga agaatataat
     6421 tactttatgg ctgtttgttt tcctgagaat caattaagaa ttatggatta taatcgtgtg
     6481 gtaaaagatt tgaatcattt gactgatgaa gaattcttaa aaaaaatttc agaatatttt
     6541 ctggttgaaa aaaaaggaaa agaaatttat cagccaaata aattacataa tttttcgtta
     6601 tacttagcag gcaactggta ttctctgaca gctaaagaag gaacttataa tgatgctgat
     6661 ccaattgaag gattagatgt tacggtgttg tcccattata ttctggataa tattttggat
     6721 attaaagatt tgcggaccga caaacgaatt gattttgtag gaggaattcg aggtttagga
     6781 gaactaaaaa aattggtcga cagtggaaaa atgaaagcag catttgcctt gtatccagta
     6841 tcgatgaaac aattaatagc aatagccgac tcaggaaata tcatgcctcc caaaactact
     6901 tggtttgagc ccaaattacg gtctggatta gtaattcatg agttggaatg acttaatgaa
     6961 aatttataaa tttcactttt ctcgcttttt aaatattatt catgcgtttt agaattttca
     7021 gcgtcatatt gattctttta gcttttctct tactggaagg atgcaatact gcttcaagaa
     7081 tcaagaaagc tgataatcgt tttgaaattg gagaatatta tgctgctggc gagttgtata
     7141 aacgttgcta tagccagctt tcttctaagg ataaacctat gcgagcttat gtggcttttc
     7201 agcaaggtga atgttaccga ttgacaaatt attatcgggc tgaacaagct tttgccaatt
     7261 ccatcagaaa taatttttcc gacagcattg tttttttaag atatgctcaa caattgcaca
     7321 aaaataggaa atatgcagat gctttgaaat attataaaat atatcttcag aaagattctt
     7381 ccaatattgt tgcaaaaaat gggattttgg cgatagattc tattgagcaa tggaaaaata
     7441 ctcctactcg ttatcaaata aattatgaaa aacaatttaa tgaacgaaag tcttcttctt
     7501 tcagcccttc ttttatgaac tcttcaaatg atgtgctggc gtttacttct actcgtccga
     7561 tgaataaaaa agctcctcct aaagccgatc ctattactgg cttacctaaa aataacttat
     7621 taatagtaaa aaagaatgcg atcgggaaat gggaaaaacc tgagtttatc gaaggtgaga
     7681 ttaatacctt agattccgac gaaggtgttt gttccttcag ctcagatggt aaaactatgt
     7741 atttttctcg agctcaacaa tcacaagaaa aagatatggg agctgaaatt tattattcta
     7801 aacgtgcagg tggaagttgg agcacacctc aaagaattac tatctttaaa gattcaagca
     7861 tttcagtagc tcacccagca attgcacccg atggtgaaac actttatttt gtttctgatc
     7921 atccttctgg ttttggagga aaagatattt atcgagctgt tttagaaagc ggcgaatgta
     7981 aatatgtaga aaatttaggt ccttcaatta acacaccagg agatgaaatg ttcccgacaa
     8041 taagaattga tggaacactt tatttttctt caaatggtca tcctggtttt ggggggctcg
     8101 atatttttaa agctgtacaa aacaaagatg gtatgtggca aataaaaaac atggggatgc
     8161 cagtcaattc ttcttcagac gatttcggaa ttacttttga aggaaatgcc gaacgaggtt
     8221 atttttcttc aaacagaaat gaaaatcgcg gttatgacgc catttggagt tttgaattac
     8281 cagaaataat ttatttattg gaaggaaaag tagttgatga aaacggacag cctgtcccag
     8341 atgcaaaaat tagaattgta agtaatacag gagtcaatgc ttatacacaa actaaaaaag
     8401 acggttcgta tcgttttaaa ttggaaaaag atgcagagta tgttatgctg gcttctgcac
     8461 gaggatacct aaatcagcaa aataaattgt caactatggg tgttcaagaa agtaaaacat
     8521 atcacgttga ttttaaatta tatccaatta ataaacccat caaaattgaa aatatttttt
     8581 atgaatttgg gaaatgggat ttgacacccg cttctgaaaa agccttgcaa gaattagtgc
     8641 aagtattaaa agataatcca aacattacta ttgaattaag ttctcatact gattatgtgg
     8701 gagataatca attcaataaa attctttcag aaaaaagagc tcaatctgtt gttgattatt
     8761 taattaagca tggaatagaa aaagaacgat taacagcagt aggttatgga gaagaacaac
     8821 ctgtggtagt agatgaaaat ttggttcaaa aatatccatt tttaaaactg ggacaacctt
     8881 taactgaaag ttatattttg actttgacgc ctgaacaaca ggaaattgcc aatcaaatta
     8941 atcgacgaac agaattcaaa gtgcttaaaa caacctataa tttatattga taaaaataaa
     9001 atatttattt cttaaaaaat gaaacaatat ttacatttat tgaataaagt acttaccgaa
     9061 ggggtttaca aagaagatcg aaccggagta ggaactatca gcatttttgg acatcaaatg
     9121 cgttttttgt tagaggaagg ttttccttgc ctcactacca aaaaattaca tttaaaatct
     9181 attatttatg aattactctg gtttttaaaa ggagacacca atgtgaaata tctgcaagaa
     9241 aatggcgtaa ccatctggga tgaatgggca gataaaaatg gagatttagg tcccatttat
     9301 ggctaccaat ggcgttcatg gcctgattat aatggcggat ttatcgatca aatttcagaa
     9361 gcagttaata caataaaaaa taaccccaat tctcggagaa tagttgtaag tgcctggaat
     9421 gtggctcaac ttcctcaaat gaaattacca ccctgtcata tactttttca attttatgtg
     9481 gtgaatggca ctttaagctt gcaactttac cagcgaagtg ctgatatttt tctgggagtg
     9541 ccttttaata ttgcttctta ttcattatta ttaatgatga tggcacaggt tacaggtttg
     9601 aaagcaggtg aatttattca tactttaggt gatgcccata tttatctgaa tcatctggat
     9661 caagtaaaat tacaattaac tcgtattcct cgtcctttac ccactatgaa gatcaatcct
     9721 gatgttaaag atattttttc gtttcagtat tctgattttg aacttattaa ttatgatcct
     9781 tatcctccca ttaaaggtga agtagcagta taaaaaagaa ttgattaatt tctttccaga
     9841 aaaatcaaaa aacaattttt tttataaacg ttgagaactt tcatattttt tagtaacttt
     9901 gaaaaattgt cttaacgctt attaacattt aaagtgatgc atcatgccta aaatttcaat
     9961 cattgctgct gttgctgaca attatgcaat tggaaatttg aataaacttc catggcattt
    10021 gccagcagat ttgaaatatt ttaaacagct tactacaggt catcctatca ttatgggaaa
    10081 acgcacattt gataatttgc caaatggacc gcttccaaat agaaaaaaca ttgttttgac
    10141 ttctgttatc tccgaaggaa tcacaaaagg gtatgtagaa gctaattcat tggaagaagc
    10201 tcttgagata tgcaaaaatg atgatgaagt atttattatt ggtggcgttt cagtatttaa
    10261 acaagccatt gatatcgctg acagaatgta cattacgtgg gtacatgaaa attttaaggc
    10321 agatgccttt ttcccagaaa ttaattttga cgaatgggaa gaaatatgca gagaagatca
    10381 ccctgctgat gataaaaacc cttacgctta tactttctct gtttacgaac gaaaaaaaag
    10441 aaaataagat tttccttttt atcctatgcc tgatggtttt ttaattttaa tgtagctgga
    10501 gaaaactaaa aatcaactaa tgttaaatga gta
//
LOCUS       DAOSAS010000057        10436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4221_length_10436_cov_12.758886, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000057 DAOSAS010000000
VERSION     DAOSAS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10436
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4221_length_10436_cov_12.758886"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..1139)
                     /locus_tag="PLR72_03950"
     CDS             complement(258..1139)
                     /locus_tag="PLR72_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36394.1"
                     /translation="MNQSHKSITIYTTEPSKIVLNHDTINTIDNKVNLNVERKRELIK
                     ITALTDSLTKEFEVEPKNSFMYWSNIFCNYGIGMLVDRKNPKRYSYPQRIYINSTDTI
                     SRYYRYGQANNKGELHLHISLPHINSFNLNPENEETKTNTGFWGLTVGLDYYHSKNQF
                     LNVGISGVSDFFVPFPAAVDISGEYELMSSRYASISNNHRLVRFSLGYGLSYAKNIWD
                     FRYYDRFDPPPPTREPIKRSHNAIGLVFPTYFQLGDHFHFGVVYRPTFFRPNMTDKFN
                     YEHLISFDFAWKIRVKK"
     gene            complement(1356..2375)
                     /gene="ruvB"
                     /locus_tag="PLR72_03955"
     CDS             complement(1356..2375)
                     /gene="ruvB"
                     /locus_tag="PLR72_03955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOK36395.1"
                     /translation="MEEEKFDIRNNRNIDKEFENILRPITFSDFKGQNKIVENLKIFV
                     QAARMRNEPLDHVLLHGPPGLGKTTLSNIIANELNVGFKLTSGPVLDKPGDLAGVLTS
                     LEPNDVLFIDEIHRLSPVVEEYLYSAMEDFRIDIMIDKGPSARSIQINLNPFTLIGAT
                     TRSGLLTSPLRARFGINCHFEYYDDLVITAIVKRSARLLDIPIEDEAAAEIGNRSRGT
                     PRIANALLRRVRDFAQVKGTGKIDLKIARYALEALNIDKYGLDEIDNKILTTIIDKFN
                     GGPVGLTTIATALGEDPGTLEEVYEPFLIMEGFLKRTPRGREVTNLAYKHLKKNRLGE
                     MGTLF"
     gene            complement(2385..3293)
                     /locus_tag="PLR72_03960"
     CDS             complement(2385..3293)
                     /locus_tag="PLR72_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK36396.1"
                     /translation="MSITISHLSKSFGKQKVLNNISFEIKQGDIVGFLGPNGAGKTTT
                     MKIITGALPYREGSVKVDGWEVKEQPLKTKSLIGYLPEQNPLYAEMYVKEYLLFVASL
                     YGLRKNKNEKIDKLIDVVGLRPEFKKRIGQLSKGYRQRVGLAQALIHDPKVLILDEPT
                     TGLDPNQLEEIRELIRSLGKNKTVLLSTHILQEIKAICNRVIVINKGEIVADYENFSK
                     INNADKNHFLYEIEFSKAVEMEKLKNLSNIISIKTIHLSHFILSSSSDIRSELFNFAV
                     ENENPILTLKPIEKSLEEIFKELTTE"
     gene            complement(3587..4258)
                     /gene="pssA"
                     /locus_tag="PLR72_03965"
     CDS             complement(3587..4258)
                     /gene="pssA"
                     /locus_tag="PLR72_03965"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOK36397.1"
                     /translation="MKKNIPNFITCLNLFSGCIAVYFAFQGNYAIVLLFVFIAAFFDF
                     LDGLMARLLNAPSALGKELDSLSDIVSFGLTPGAIAFSLLQQTNLPFWLAFTGFLLPI
                     FSALRLAKFNLDTRQTNYFLGLPTPAHAIFWAGLSYSFSFFLIENYWITLTLIVLFSG
                     LLVSEIPMFSLKFKTLRWEGNEIQYIFILVSIVLFAVFLQDSFSLIICWYILLSLLTP
                     KIFKE"
     gene            complement(4282..4737)
                     /gene="dtd"
                     /locus_tag="PLR72_03970"
     CDS             complement(4282..4737)
                     /gene="dtd"
                     /locus_tag="PLR72_03970"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOK36398.1"
                     /translation="MRVLIQRVDEALVTVDGEKIAAIGKGLLAFVGIEENDTEKDAEW
                     LAGKTASLRIFDDEKGIMNLSVMDINGEILVVSQFTLCAMTHKGNRPSYVKAAKPGKA
                     IPLYEFFCQTLSTKIGKQVKTGIFGAHMKIALVNNGPVTIWIDSKNPLS"
     gene            complement(4784..5884)
                     /gene="ychF"
                     /locus_tag="PLR72_03975"
     CDS             complement(4784..5884)
                     /gene="ychF"
                     /locus_tag="PLR72_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOK36399.1"
                     /translation="MGLQCGIVGLPNVGKSTLFNCLSNTKAQAANFPFCTIEPNIGVI
                     TVPDKRLNKLAELVHPQKIVPTTVEIVDIAGLVKGASKGEGLGNKFLANIRETDAILH
                     ILRCFDDENVAHVNGKVDPVRDKEIVDMELQLKDLETIESRINKVQKQAQTGGDKQAK
                     RICEILLQYREALLQGKSARTVKLDKEEKKLVKDLYLLSDKPVMYVCNVDENSIHNEN
                     AYVKAVRESIKDENAEILVICARLEAEIAELETYEERQMFLEEWGLTESGVSRLIKSA
                     YRLLNLETFFTVGPQEVRAWTYEKGWKAPQCAGVIHTDFEKGFIRAEVIKYDDFIRLG
                     SEAACREAGKIYIEGKDYEVQDGDIMHFRFNV"
     gene            complement(5978..6606)
                     /gene="purN"
                     /locus_tag="PLR72_03980"
                     /pseudo
     CDS             complement(5978..6606)
                     /gene="purN"
                     /locus_tag="PLR72_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307440.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
     gene            6706..6942
                     /locus_tag="PLR72_03985"
     CDS             6706..6942
                     /locus_tag="PLR72_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOK36400.1"
                     /translation="MSEVEEKVKAIIAEKLGVDEAEITPSASFTNDLGADSLDTVELI
                     MEFEKEFGISIPDDQAEKITTVGDAINYIENLKK"
     gene            6956..8218
                     /gene="fabF"
                     /locus_tag="PLR72_03990"
     CDS             6956..8218
                     /gene="fabF"
                     /locus_tag="PLR72_03990"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOK36401.1"
                     /translation="MELKRVVVTGLGAVTPVGTTVPIFWNNIVNGVSGAAPITHFDAS
                     QFKTQFACEVKDFDPTQYFEKKEIRKMDLYTQYGLVAAKEAVENCGVDVEKVDKNEIG
                     VIFSSGIGGIATYEQEVSNYYLNKDAGPKFNPFFIPKMISDIAAGQISIQYGFHGPNF
                     ATTSACASSTNSLIDAFNYIRLGKAKMFVVGGAEAAITPAGIGGFNAMMAISTRNDDP
                     KTASRPFSKSRDGFVMGEGAGCLILEELDFALARGANILCELVGGGLSADAYHLTASH
                     PDGLGAILAMSNALKDAELSPTDIDYINVHGTSTGVGDISEVKAIKEVFGEHAYKLNI
                     SSTKSMTGHLLGAAGAVEAIISILSLINGIVPPTINFTEGDEDPEIDYRLNFTFNQAQ
                     KRELNAVLSNNFGFGGHNATIIFKKYTK"
     gene            8223..8981
                     /gene="rnc"
                     /locus_tag="PLR72_03995"
     CDS             8223..8981
                     /gene="rnc"
                     /locus_tag="PLR72_03995"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOK36402.1"
                     /translation="MINLFSKKLRLFSRKRKEPYSLFYKVLGFYPHNIDYYQLAIRHK
                     SAPIITKNGKILSNEHLEFLGDAVLNSIISDILFLHYKNKDEGFLTTTRSKIVNRFTL
                     NRLSLELGLQNLVKIAKHVNVNNINDIYGNALEALIGAIYLDKGYENSKQFVKKRLLC
                     LLDLEKLEKEEINYKSKLIEWCQKNKIPIEFEVVSENLSKDNKHVFHSHVLIDGKIIS
                     ESEGYSKKESQQNASRLAYEQIILRLHVQKQEIF"
     gene            9101..9301
                     /locus_tag="PLR72_04000"
     CDS             9101..9301
                     /locus_tag="PLR72_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOK36403.1"
                     /translation="MLTEKIGTNAGLVWNILHENGKMEVKALKKAAKLTEKDLNMALG
                     WLAREGKIQFNEQENELFVSLI"
     gene            9429..9501
                     /locus_tag="PLR72_04005"
     tRNA            9429..9501
                     /locus_tag="PLR72_04005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9462..9464,aa:Gly,seq:gcc)
     gene            9534..9618
                     /locus_tag="PLR72_04010"
     tRNA            9534..9618
                     /locus_tag="PLR72_04010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9567..9569,aa:Leu,seq:taa)
     gene            10023..>10436
                     /locus_tag="PLR72_04015"
     CDS             10023..>10436
                     /locus_tag="PLR72_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOK36404.1"
                     /translation="MKLSNKYNPEGIESKWYQYWLEFGFFHSTPDEREPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDILVRRARMLGKNACWVPGTDHASIATEAKVVAYLAKKGIKK
                     SDLTREEFLDYAWKWTRKHGGIILEQLKKLGASCDW"
BASE COUNT         3436 a         1766 c         1758 g         3476 t
ORIGIN      
        1 gagaaactga cctcctaacc attagaggaa cgcaccagtt gaaaatcatt gcaaagataa
       61 aggataattt ttgaattttc cacagaaaag gacaaaagtt cttttatttt tcgcatgatt
      121 ttatcgtttt caggctttgc gttcgggcgg gtttcggatt acttcactgt caaccagcac
      181 tgaacttgaa tagaagcaca aagctccaag tttgcacgtc accccgcctg acgcaagacc
      241 cgtgttacca gccgtaatta tttctttact cttattttcc acgcaaaatc aaaacttatt
      301 aagtgttcat aattgaattt gtcagtcata ttaggtctga aaaaagtcgg tctatagaca
      361 accccaaagt ggaagtggtc acctaactga aaatatgttg gaaataccaa gccaattgca
      421 ttatgacttc tttttatcgg ttctcttgtc ggtggtggtg ggtcaaatct gtcataatat
      481 ctaaagtccc aaatgttttt tgcgtaggat agtccgtatc ctaatgaaaa gcgtacaagt
      541 ctatgattat tagagatact ggcatatctt gaactcatca attcatattc accacttatg
      601 tcgactgctg ctgggaatgg aacaaaaaag tctgagacac cagaaatccc aacatttaag
      661 aattggttct ttgaatgata atagtcaagt cctactgtta atccccaaaa tccagtatta
      721 gttttagttt cttcattttc agggtttaga ttaaatgaat ttatgtgtgg caatgatatg
      781 tgcaggtgta attctccttt gttgtttgct tgtccatatc tgtaatatct gctgattgta
      841 tctgtagagt ttatataaat tctttgcggg taagaataac gtttaggatt ttttctgtca
      901 acaagcattc caatgccata attgcaaaaa atgttgctcc aatacataaa agagttcttt
      961 ggctcaactt caaattcttt ggtcagacta tctgtcaaag cagtaatttt tattagttct
     1021 ctttttctct caacattcaa atttactttg ttgtcaatcg tgttaattgt gtcatggttt
     1081 aaaacaattt tgctcggttc ggttgtataa atagttatac ttttatgcga ctggttcata
     1141 attgtcgcgc aagaagtcaa aagaattgaa actgtcaata tgtatagtgt ctgtctcata
     1201 ttatggctag taacgtatag tttttctaat taaatttcaa ttttctgact ataaacggct
     1261 taaaatatga aatatatgat atttcattag tgaatattgt tttataaaaa atctacgaaa
     1321 ttcaaaatca ttctcaacaa ttttatcaca atttatcaaa acaaagttcc catttctccc
     1381 agtctgttct ttttcaaatg tttgtaggct aagttggtaa cttcacgtcc acggggagta
     1441 cgtttgagga agccttccat gatcagaaaa ggttcatata cttcttccag tgttccagga
     1501 tcttcgccga gagctgtggc aatggtggtt aagccaacag gaccaccatt aaatttatcg
     1561 ataatcgtgg ttagaatttt gttgtcgatt tcatccaaac catatttatc aatgtttaaa
     1621 gcttccagtg cataacgtgc aattttcagg tctatttttc cagtaccttt cacctgagca
     1681 aaatcacgta cacgacgtaa taatgcattg gcaatacgtg gagtcccgcg gctacggtta
     1741 ccaatttctg cggctgcttc gtcttcaatg ggaatatcaa gcaaacgagc agagcgtttt
     1801 acaatagcgg taataaccaa gtcatcatag tattcgaaat gacaattaat accaaatcta
     1861 gctcgcaaag gagaagtgag caaaccgctt cgggtagttg ctccaataag cgtgaaagga
     1921 ttcaaattta tttgaatgga acgggcactt ggacctttat ctatcataat atcaattctg
     1981 aaatcttcca tcgcagaata taaatattct tctactactg gactcagacg atgaatttca
     2041 tcaataaaga gcacatcatt tggctcaaga ctggtaagta cgccagctaa atcacctggc
     2101 ttatccagaa caggtccaga agtaagttta aaaccaacat tcagctcatt ggcaataata
     2161 ttagaaagag tagttttccc caatccagga ggtccatgca ataagacatg gtctaaaggc
     2221 tcattacgca tacgagcagc ttgaacgaaa attttcaaat tctcgacaat tttgttttga
     2281 cctttgaaat cagaaaaggt aattggtcgc agaatatttt caaattcctt gtcaatgttt
     2341 ctattgttac gtatgtcaaa tttttcctct tccatgttga ttaatcactc agtagttaat
     2401 tctttgaaaa tttcttccaa acttttttca atgggcttta aggttaaaat ggggttttca
     2461 ttttctactg caaaattaaa aagttcactt cgaatatcag aagaagatga taaaataaaa
     2521 tgagacagat gaattgtttt tatagaaata atattagaga gattttttaa tttctccatc
     2581 tctactgctt ttgaaaattc aatttcatac aggaaatgat tcttatccgc attgtttatt
     2641 ttggaaaaat tttcataatc agctacaatt tcgcctttat tgataactat tactcggttg
     2701 caaatggctt taatttcctg taaaatatgt gtactgagca aaacggtttt gttttttccc
     2761 agtgaacgaa tcaattccct gatttcttcc agttgattgg gatcaagtcc agtagtaggc
     2821 tcgtctaata ttagtacttt gggatcgtgt atgagggctt gtgccaatcc tactcgttga
     2881 cggtaacctt ttgaaagttg accaattcgt tttttaaatt ctggacgaag tcccacaaca
     2941 tcaattaatt tatcaatttt ttcgttttta tttttcctca agccataaag agaagccaca
     3001 aaaagcaaat attctttcac gtacatttct gcataaagcg gattttgttc cggcaagtat
     3061 ccaataagcg atttagtctt caaaggctgt tcttttactt cccaaccatc cactttgaca
     3121 gaaccctctc gataaggcaa tgctcccgta ataattttca tggtagtagt tttccctgct
     3181 ccattgggtc caagaaaacc cacaatatcg ccttgtttta tttcaaagga aatattgttt
     3241 aaaacttttt gtttgccaaa actttttgaa agatgggaaa tagtgataga cattttaaaa
     3301 attattcaaa aattcttttc ttaaaaaatt gttatcaata aaataaacca aataataaaa
     3361 attacaagaa acaaaagtac taaaaaatac tgattctgtt tctaacctca aagtactttg
     3421 atggttatac tttgttactc atttttataa agttaacaaa attagaataa aggtaagaga
     3481 aagtttttta ttctttattt ttttattaat tcagtttatc gagttgagaa gcaattaaca
     3541 taaaggaaat attacatttt ttctttttta ggtgataatc agtaaattat tctttaaaaa
     3601 tttttggtgt caacaaggaa agcaaaatat accagcaaat aatgagtgaa aaagaatcct
     3661 gcagaaaaac agcgaaaagg acaatactta ccagtataaa aatatattga atttcattcc
     3721 cttcccatct tagcgtctta aattttaaag aaaacatagg aatttcactt accaataatc
     3781 cactgaaaag aacaattaat gttaatgtaa tccagtaatt ttcaatgaga aaaaaagaaa
     3841 atgaataact taaccctgcc caaaaaatag catgagctgg tgtaggtaat cctaaaaaat
     3901 aattagtttg gcgggtatcc aggttgaatt tagctaatcg caaagctgaa aaaataggaa
     3961 gcaaaaaacc agtaaaagcc agccagaaag gcaaattggt ttgttgaagt aaagaaaaag
     4021 caatggcgcc tggtgtcaag ccaaaactga caatgtcgga taaagagtct aattcttttc
     4081 ctaaagcaga aggagcattt agcaaacgtg ccattaagcc atctaaaaaa tcaaaaaatg
     4141 ctgcaatgaa aacaaagagc aaaactatag cataatttcc ctgaaaagca aaatatacag
     4201 caatacatcc cgaaaataaa ttcagacagg taatgaagtt aggaatattc tttttcatac
     4261 tttttgaaaa taagtgttta tttatgaaag aggatttttt gaatcgatcc agatggtcac
     4321 cggtccatta ttgactaagg caattttcat gtgagctcca aaaatgcctg tttttacttg
     4381 tttcccaatt tttgtgctta atgtttgaca aaagaattca tataagggaa tggcttttcc
     4441 tggttttgct gcttttacat acgaaggtcg attgcctttg tgagtcatgg cacaaagagt
     4501 aaattggctt acaaccagaa tttctccatt tatatccatt acggataaat tcattattcc
     4561 tttctcatca tcaaagattc gtagcgaggc tgtttttcca gccaaccatt ctgcatcttt
     4621 ttcagtatcg ttctcttcaa tcccgacaaa agctaaaagt cctttcccaa tagcagcaat
     4681 tttttcacca tcaactgtca ccaatgcttc atctactctt tgaatcaaaa ctcgcatata
     4741 agatttatat ttaaagcgaa tctaaattgc ttttaaaaaa catttaaaca ttgaatcgaa
     4801 aatgcatgat atcgccatcc tgaacctcat aatcttttcc ttcgatgtaa attttcccag
     4861 cttcacgaca tgctgcttct gaacctaaac ggataaaatc atcatatttt atgacttctg
     4921 cccgaatgaa gcctttctca aaatcggtat gaataactcc agcacattga ggagctttcc
     4981 aacctttttc ataagtccaa gcacgcactt cttgtggacc tactgtaaag aaagtttcca
     5041 gattcaataa tcggtaagcc gatttaatca atcgtgaaac gcctgattct gttaatcccc
     5101 attcttccag gaacatttga cgttcttcat aggtttccag ttcagcaatt tcggcttcca
     5161 atcgagcaca gatgactaaa atttccgcat tttcgtcttt tattgattca cgaacagctt
     5221 ttacgtaagc attctcgtta tgaatggaat tttcatctac attgcaaaca tacattactg
     5281 gcttgtcgct cagtaaataa agatctttga caagtttttt ttcttcttta tccagtttaa
     5341 cggtgcgcgc tgatttccct tgtagtaaag cttcgcgata ttgaagtaaa atttcacaga
     5401 ttcttttagc ttgcttatca ccacctgttt gagcttgttt ttgtacttta ttaattctgc
     5461 tttcaatggt ttccaaatct ttcaactgaa gttccatatc tacaatttct ttgtctcgaa
     5521 caggatcaac ttttccattc acatgagcaa cattttcatc atcaaagcat ctcaatatat
     5581 gtaatatagc gtctgtttca cgaatattgg ctaaaaattt attccccaag ccttctcctt
     5641 tgctcgctcc ttttaccaat cccgcaatgt ccacaatttc tacagtagta ggtacaattt
     5701 tttggggatg aaccagttca gctaatttgt tgagacgctt atctggtacg gtaatcactc
     5761 cgatatttgg ttcgattgta caaaaaggaa aattggcagc ttgagctttt gtatttgata
     5821 aacaattgaa taaagtagat tttcccacat taggcaaacc tacaattcca cattgaagac
     5881 ccatgaaata aaaaattagt tggtaaaatc ttaagcaata tttcattgca aagatacaca
     5941 aaatctctca aagctttttg ttcaaacaat aatggagtta gttttgctga ccccagattt
     6001 tttcaatcac tcgaggaaaa tattgatatt ctaattcatg aacctttttt gcaattgttt
     6061 caggtgtatc ttcgggaagt acaggacatt ttgcttgaaa aatgatttca cctgcatcgt
     6121 attcattatt tacgtaatga atagtgatgc ctgtttcggt ttctcctgct tcttttactg
     6181 cctcgtgaac tcgcattcca tacattcctt ttccaccaaa ttttggaagc aaagcaggat
     6241 gaatattaat gattttttgt ggataagcat ctatcaccaa cgatggaact tgaagtaaga
     6301 aacctgcaag aactatagca tctatctttt gctcctgtaa aaaattcaat attttttctc
     6361 cttgtaaaaa atcttctttt gaaaatgtaa ccgaagatac tccaagtttc tttgctcgct
     6421 catgaacaaa agctttttca ttatttgaaa taatgactgg aaaaacaaaa tttggatgat
     6481 tagaaaaata tcgaataata ttttcggcat ttgaaccaga acctgaggca aaaattgcaa
     6541 ttctaatggg cataatttta agttaaatac tgcacaaaat taggcaaatt gtgcaaaaaa
     6601 gtgcattttt ttcaataaaa cgatggttat ttgaagaaaa ttttatttct ttgcagcaca
     6661 aaattataaa acaaatttta tttataaact taaaatttaa aaattatgtc agaagttgaa
     6721 gaaaaagtaa aagcgattat tgccgagaaa ttaggcgtag atgaagctga aatcacacct
     6781 tcagcaagtt ttacaaatga tcttggtgca gattcgttag ataccgttga actcattatg
     6841 gaatttgaaa aagaatttgg tatctctatt cctgacgatc aagcagaaaa aattacaaca
     6901 gttggtgatg ctatcaacta tattgaaaat ttgaagaaat aataagtttt ctcttatgga
     6961 gttaaagaga gtagttgtaa caggattagg agcggttaca ccagtgggaa cgacagttcc
     7021 cattttttgg aacaacatag ttaatggtgt aagtggagct gcgcctatta cccattttga
     7081 tgcttctcaa tttaagacac aatttgcttg cgaagtaaaa gattttgatc ctacccaata
     7141 ttttgagaag aaagaaattc gcaaaatgga tctttatact caatatgggt tagtagcagc
     7201 caaagaagct gtagaaaatt gtggggtaga tgtagagaaa gtcgataaaa atgaaattgg
     7261 ggtgattttt tcttctggaa taggaggcat tgccacttat gaacaagaag tgtctaatta
     7321 ttatctcaat aaagacgctg ggccaaaatt caatccattt tttattccaa aaatgatttc
     7381 cgatattgca gcaggacaaa tttctattca atatggtttt catggtccta attttgctac
     7441 cacttctgct tgtgcttcgt caaccaattc attgattgat gcttttaatt atattcgttt
     7501 aggaaaagcc aaaatgtttg ttgttggagg tgctgaagca gctattacac cagcaggcat
     7561 tggcggattt aatgctatga tggcaatatc aaccagaaat gacgatccaa aaactgcttc
     7621 acgacctttt agcaaaagtc gtgacggttt tgtcatggga gaaggagctg gttgtttgat
     7681 tttggaagag cttgattttg cacttgctcg cggagcaaac attttatgtg aactcgtagg
     7741 tggaggttta tcagctgacg cttatcattt aacagcttct catccagatg gtttgggagc
     7801 tatcctggca atgagtaatg ctttaaaaga tgcagaatta tctccaactg atattgatta
     7861 tattaatgtt catggtactt ctacaggcgt tggtgacatt tcagaagtta aggcaattaa
     7921 agaagttttt ggcgaacatg cttataaact gaatattagt tcgacaaaat ctatgacggg
     7981 gcatttatta ggtgcagctg gagcggttga agcaatcatt tctatacttt ctctaataaa
     8041 tggaattgta ccacctacca ttaattttac ggaaggcgat gaggatccag aaattgatta
     8101 tcgtttgaac tttacattta atcaagctca aaaaagagag ctcaatgctg ttttatccaa
     8161 taattttggc tttggtggac ataatgcaac aatcattttc aaaaaatata ctaaataagc
     8221 ttgtgataaa tcttttttca aaaaaattaa ggctcttttc tcgcaaaaga aaggagcctt
     8281 attctttatt ttataaggtt ttaggatttt atccccacaa cattgattac tatcaactgg
     8341 caatacgaca taaatcggct ccaatcatta ccaaaaacgg caaaatatta agcaacgagc
     8401 atttggaatt tttaggagat gctgtgttga attccatcat ctcagatatt cttttccttc
     8461 attacaaaaa caaagatgaa ggatttctta ctactactcg ttctaaaatt gtaaatcgat
     8521 ttactttaaa tcggttatct cttgaattgg gactgcaaaa tttggtgaaa atagctaaac
     8581 atgtaaatgt caacaatatt aatgatattt atgggaatgc attagaagct ttaattggcg
     8641 ctatttatct tgataagggt tacgaaaata gcaaacaatt tgttaaaaaa agattacttt
     8701 gtttattgga tttggaaaaa cttgaaaagg aagaaataaa ttataaatcc aaattaattg
     8761 aatggtgtca gaaaaataaa atacctattg aatttgaagt tgttagtgaa aatttaagca
     8821 aagacaataa acatgttttt cattctcatg tgttgattga cggaaaaatt atttctgaat
     8881 cagagggcta tagtaaaaaa gaatcacaac aaaatgcttc cagactggct tatgagcaaa
     8941 taattttgcg cctccatgtt caaaaacaag aaatctttta aactgaaatt attcataatg
     9001 agtgctttag ttcttcgatc ctttgatttt gatattcatg gtatttttaa taactttgca
     9061 aaataaaact tataattaac aattaaaaag ctaaaccact atgttaacag aaaaaattgg
     9121 aacaaatgct ggtcttgtat ggaatattct ccatgaaaat ggaaaaatgg aggtaaaagc
     9181 cttgaaaaag gcagctaaat taactgaaaa agatttaaat atggctttag gttggttagc
     9241 tcgtgaggga aagattcagt ttaatgaaca agaaaatgaa ttatttgtaa gtcttattta
     9301 agcgagaaaa ttaaggcggt tctgttgatg tcccgccttt ttaattttaa aattactttc
     9361 aaattttacc tcaaaaaaat ttcataaacg aaaaaatgag tgtatatttg tactcacttt
     9421 tattcaacgc gaaaatagct cagttggtag agcacgacct tgccaaggtc ggggtcgcgg
     9481 gttcgagtcc cgtttttcgc tcactaaaaa aattaatttt gaagaggatt gttgcccgga
     9541 tggtggaatg gtagacacga aggacttaaa atcctttggc cctcaaaggc tgtgcgggtt
     9601 caagtcccgc ttcgggtaca aataatttaa tatcatagtt taatcaatta actatgatat
     9661 tttttatgtg gaaattaata aattaattat tatcatataa atttgaaaat taattcggtg
     9721 tatttgtact tctctggtaa tcgaaaaaaa tgaaaagcca aaattatttt gaagtaacaa
     9781 ttgaaatatg gcgttaacat aaaactttaa tgaataattt atctttcaaa atttcttcga
     9841 taaaacaaac ttttttattg tgttctttgc cctctaaatg aagcttactt gcattctttt
     9901 tttaaatttc gtttttataa gcctttcctt ttatttatct ttgcaaattt acaattgaaa
     9961 aaatcatcag catttttaat gacctcaaaa aatgttgatt attttattaa aacaatcatt
    10021 ggatgaaact ttctaataaa tacaacccag aaggaattga gtcaaaatgg tatcaatatt
    10081 ggttagagtt tggttttttt cattcaacac ctgacgaacg tgaaccttat accattgtta
    10141 ttccaccacc caatgtgaca ggtgttttac acatgggaca catgttgaac aataccattc
    10201 aggacattct ggttcgccga gctcgtatgt tggggaaaaa tgcctgttgg gttccgggca
    10261 ccgatcatgc ttccattgcc acagaagcaa aggtagtagc ttatctggct aaaaaaggta
    10321 taaaaaagtc tgatcttaca cgggaagaat ttttagatta tgcatggaag tggactcgaa
    10381 aacatggtgg aattattctg gaacaattaa aaaagctggg tgcttcgtgc gattgg
//
LOCUS       DAOSAS010000058        10112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4366_length_10112_cov_13.235856, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000058 DAOSAS010000000
VERSION     DAOSAS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10112
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4366_length_10112_cov_13.235856"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="PLR72_04020"
     CDS             <1..128
                     /locus_tag="PLR72_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOK36405.1"
                     /translation="REAVDACITAGVVTEDIASGEKAYKTSEVGEWITNYILKKK"
     gene            194..1420
                     /locus_tag="PLR72_04025"
     CDS             194..1420
                     /locus_tag="PLR72_04025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK36406.1"
                     /translation="MFNNYVGKLTDNKKKALLLIFLFGIISFLGDIIYEGARSVNGPY
                     LKTLGANAANVGIVVGIGELLGYAIRLFSGYFSDKKNAAWFFTILGYSLLVSIPLLSF
                     SNFWQLAAMFIVIERIGKGLRSPAKDTLLSYATKQVGTGIGFGISEFLDQLGATLGPL
                     IFMIFFLTTGNSQNTLIDYQHAYSLLWIPFVLLLIVVLFAYFKVKTPETLESSENQPT
                     GFTKVFWFYSLFTFITTFGFINFAIIGFHLKTKAILPDSQIPLLYTIAMVIDAIFALI
                     VGKIYDKYKALHHNEQGGLLILVFIPIITIFILPLAFSKHLVLIITSMVFWGIVMGTH
                     ETIMKAAIADITSINKRGTGYGIFNLIYGLALFGGSLVAGYLYEISIPLLISIFIFVE
                     ILSVPFFFLLKKEIKR"
     gene            complement(1506..3731)
                     /locus_tag="PLR72_04030"
     CDS             complement(1506..3731)
                     /locus_tag="PLR72_04030"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOK36407.1"
                     /translation="MENNIFFLCPTAGVLALLFAWYFSHQMMKASEGTVTMKTIAKHV
                     REGAFAYLKQQYKVVAIVFLCLTILFAIMAYGFNIQNPWVPFAFLTGGFFSGLAGYIG
                     MRTATYASARAANAAQHSLNSGLKIAFRSGAVMGLVVVGLGLLDISIWFYVLQFFIGA
                     LDNTTDLAIAADPSMKLIIITTTTLTFGMGASTQSLFARVGGGIFTKAADVGADLVGK
                     VEANIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYCGSILSTAALGATAFAGFGT
                     TTQFNGVIAPMIIASVGIILSILGIFLVRSKEDASQKQLLNALNRGIYTTAVLIAIFS
                     FFILKYLNIPNSLGVWGSMLAGLLGGVAIGKITEYFTSAAYQPTRRISEASKTGPATV
                     IISGIGTGMISTALPVIVVAISIILAYYFASGLDFTNISMGLYGIAIAAVGMLSTLGI
                     TLASDAYGPIADNAGGNAEMSGLGPEARKRTDALDALGNTTAATGKGFAIGSAALTAL
                     ALMASYLEETKVAIGRIPEKAQLFFDTVNTNIHQASIPQLMEFFQVNLMNPRVLVGAF
                     IGAMAAFLFCGLTMNAVGRAAQSMVEEVRRQFREIVGIMEGKAEPDYARCVAISTKGA
                     QKEMVFPSLLAILIPIITGIFFGVPGVMGLLLGSVSTGFVLAIFMANTGGAWDNAKKY
                     IEEGNFGGKGSENHKAAIVGDTVGDPFKDTSGPSLNILIKLMSMVSIVMAGLTVTWSL
                     M"
     gene            complement(3743..4978)
                     /locus_tag="PLR72_04035"
     CDS             complement(3743..4978)
                     /locus_tag="PLR72_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOK36408.1"
                     /translation="MVKILFFLIIFFVVADFVLERYLTYLNIQYSKQELPEILQGIYD
                     EEKYKKQQEYFRTNTRFSMLTSTLSFVIILLMLIFGGFGWLDNLLRKITEHQILLPLL
                     YFGVLFFASEIINLPFEWYDTFVIEEKFGFNKVTPKLFIVDKIKEILLSVIIGGGILA
                     TIIWIYTLTPNYFWLWAWLVVTFFSLIMNMFYSEIIVPLFNKQTPLPEGELRTEIEKF
                     ASKAGFKLSNIYVIDSSKRSTKANAYFSGLGSKKRIVLFDTLLNKLTTEEIVAVLAHE
                     IGHYKHRHTLYQFLISLPVNLLLFFLLGYILKSDIFAQVLGVASASFHINVIVFSLLY
                     APLSLLLGIFSNILSRKFEFQADAFAAQYGYATFLQSGLKKLSETSLSNLMPHPLYVF
                     FHYSHPTLYQRLTKLVSYT"
     gene            complement(5234..5899)
                     /locus_tag="PLR72_04040"
     CDS             complement(5234..5899)
                     /locus_tag="PLR72_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOK36409.1"
                     /translation="MSIKENLKEIHSQLPSSVHLVCISKFQPEAAIREAYDAGERIFG
                     ESRTQELCIKYENLPKDIQWHFIGHLQTNKIKYILPFVDLIHSVDSLKLLNEINKQTE
                     KINKTVNCLLQVHIAQEETKFGFNPDELIELFKSDEWQKYTNIKICGLMGMATFTDNI
                     TQIRNEFRGLKKLFDLLKNKYFNNNDSFSELSMGMSNDYLIAVEEGSTMVRLGTSIFG
                     ERK"
     gene            6169..7086
                     /locus_tag="PLR72_04045"
     CDS             6169..7086
                     /locus_tag="PLR72_04045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK36410.1"
                     /translation="MLSILIPTYNYNVTSLVKELHNQAINLYIDFEIIVMEDGSQYFV
                     EENENINKLTNCRHIILEENIGRAAVRNKLAEEAKYDHLLFLDCDAKICSEHFIEKYL
                     VFCKETSLVVIGGRIYEQENNLDNFSLMRKYGIIREQNTLENLKNRKKYPVLTTPNFL
                     ISKELFLKTKFDEIFSGYGHEDTIFGIKLVRNEATFFYIDNPVVHIGLETNEKFIKKT
                     ENGLKNLYNIYLSADYPEIKQLSKVLSIYLKINKIKLSKIFCFIYKHFNQNIKKRLIY
                     SLNPSLFLFDFYKLLFLCDYSFKNDKMTE"
     gene            7250..7522
                     /locus_tag="PLR72_04050"
     CDS             7250..7522
                     /locus_tag="PLR72_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOK36411.1"
                     /translation="MNIKPLADRVLVKPSPVEEKTSSGIIIPDSAKEKPLKGEVIAVG
                     NGTKDEEMTVAVGDTVLYGKYAGTELEWDGEKYLIMRQSDILAIIL"
     gene            7599..9227
                     /gene="groL"
                     /locus_tag="PLR72_04055"
     CDS             7599..9227
                     /gene="groL"
                     /locus_tag="PLR72_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617860.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOK36412.1"
                     /translation="MAKVILYDLEAREQLKEGVDELANAVKVTLGPKGRNVIMEKKFG
                     APVITKDGVSVAKEIELEDPFKNVGAQLLKEVASKTSEDAGDGTTTATVLAQAIVNVG
                     LKNVTAGANPMDLKRGIDKAVAEVVKSIANQSKQVGDDYNKIEQVATVSANNDREIGK
                     LIADAMRKVSKEGVITIEEAKGTETTIEMVEGMQFDRGYISAYFVTNAEKMLVEMEKP
                     YILIHDKKISNFKELLPILEPVAQSGRPLLIIAEDVESEALTTLVVNRLRGSLKVCAV
                     KAPGFGDRRKEMLEDIAILTGGVVITEERGLQLEKATMDMLGTAEKITVDKENTTIVN
                     GAGSKEAIQARIKQIKAQIANATSDYDREKLQERLAKLAGGVAVLYVGAPSEIEMKEK
                     KDRVEDALSATRAAIEEGIIPGGGVAYIRAIEALEKLKGENDDENTGIEIVKRAIEEP
                     LRQIVENAGKEGAVIVNKIKEGKDDFGYNARTENFENFYETGVLDPAKVARIALENAA
                     SIAGMFLTTECVITEKKEPAPVVQQPMPGMGDYE"
     gene            9755..10111
                     /gene="rnpB"
                     /locus_tag="PLR72_04060"
     ncRNA           9755..10111
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLR72_04060"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         3577 a         1571 c         1673 g         3291 t
ORIGIN      
        1 ttcgtgaagc tgtcgatgct tgtatcacag cgggcgtagt aaccgaagat attgcttccg
       61 gagaaaaagc ttacaaaact tccgaagtag gagaatggat tactaattat attcttaaga
      121 aaaaataaaa aaatttttca gaggttatta ttaaaatgaa aaattaacaa tcaaagttag
      181 aatttattga agcatgttca ataattatgt tggcaaactt actgataata aaaagaaagc
      241 tcttctttta atatttcttt ttggtatcat tagctttttg ggtgacatta tttatgaagg
      301 tgcaagaagt gtcaatggac cttatttaaa aaccttagga gctaatgctg ctaatgtggg
      361 aatagttgta ggcattggtg aattgttggg ttatgctatt cgtttatttt caggttattt
      421 ctcagataaa aagaatgctg cctggttttt taccatttta ggatatagtt tattagtttc
      481 tattccttta ttatcttttt ccaatttctg gcaactcgct gctatgttca ttgtgattga
      541 aaggattgga aaaggcttga gaagtccagc aaaggatacc ttgttatctt atgctaccaa
      601 acaagtaggg acggggattg gatttggaat ttcagaattt ctcgatcaat tgggagccac
      661 tttaggtcca ttaattttta tgattttctt tttgactacg ggcaatagtc aaaatacttt
      721 gattgattat caacatgctt acagcttgtt atggattcct tttgtgcttt tattaattgt
      781 agttttgttt gcatatttta aagtaaaaac gccggaaaca cttgaaagtt ctgaaaatca
      841 accaactgga tttacaaaag tattctggtt ttattctttg ttcactttta ttactacttt
      901 tggctttatc aattttgcta ttattggatt tcatctgaaa acaaaagcaa ttttacctga
      961 tagtcaaatt cctttacttt atactattgc aatggtaatt gatgccattt ttgctttgat
     1021 tgttgggaaa atctatgata aatataaagc tttgcatcat aacgaacaag gtggcttact
     1081 gattttagta tttattccaa taataacaat ttttattctt ccactggcgt tttcgaaaca
     1141 tcttgtttta atcattacct caatggtttt ttggggaata gttatgggga cacatgaaac
     1201 cattatgaag gctgctatag ccgatataac ttctatcaat aaacgaggaa ccggttatgg
     1261 tatttttaat ctgatttatg gtctggcttt gtttggtggt agcttagttg ctggatatct
     1321 atatgaaatt tctatcccat tattaatttc aatttttatt tttgttgaaa ttctatctgt
     1381 tcctttcttt tttcttctga agaaagagat aaagagatga agaaatagct tttaataaaa
     1441 aatccatacc tttttgtgat atggattttt ttatgataac gttttaattt tttacattaa
     1501 tttttttaca ttaagctcca ggttactgtt aatcctgcca ttacaatgga aaccatactc
     1561 atcagtttaa ttaaaatgtt gagcgagggt cctgaagtat ctttaaaagg atcaccaaca
     1621 gtatcaccga caatggctgc tttatgattt tctgaacctt ttccaccaaa atttccttct
     1681 tcaatatatt ttttagcatt atcccaagca cctcctgtat ttgccataaa aattgctaat
     1741 acaaaacctg tggaaacact acctaataat aatcccatta caccaggaac tccaaaaaat
     1801 atacctgtta taattggaat taagatagcc aataaagaag gaaataccat ttctttttga
     1861 gcccctttag ttgaaatggc aacacaacga gcatagtctg gttcagcttt gccttccata
     1921 attcctacaa tttctctaaa ttgacggcga acttcttcaa ccatactttg tgcagctctt
     1981 cctaccgcat tcattgttaa cccacaaaac agaaaagctg ccatcgcacc tataaaagct
     2041 ccaaccaata cccgtggatt cattaaattt acctgaaaaa attccataag ttgaggtata
     2101 gaggcttgat gaatattagt atttactgta tcaaaaaaga gttgtgcttt ttcaggaatc
     2161 cgtccaatag caacttttgt ttcttcaaga taagaagcca ttaaagccag agcagttaaa
     2221 gcagccgaac caatagcaaa accttttccc gttgcagcag tcgtatttcc taaagcatcc
     2281 aatgcatctg tccgtttacg ggcttctggt cctaaaccac tcatttctgc atttccacca
     2341 gcattatcag caataggacc ataggcatca gaagcaaggg taatgccaag cgtcgaaagc
     2401 attcctacag ccgcaatagc aattccatat aatcccatac taatattagt aaaatcaaga
     2461 cctgaagcga aataataagc taaaataata gaaatagcta ctacaattac cggtaaagcg
     2521 gtagaaatca taccagttcc aattcctgaa atgataacag tagcaggtcc tgtttttgaa
     2581 gcttcagaaa ttcgtcgagt tggttgataa gctgctgaag taaaatattc agtaatctta
     2641 ccaattgcaa cgccacctaa taaacctgcc aacatagagc cccatacacc tagagaattt
     2701 ggaatattta aatatttcaa aataaaaaat gaaaaaatag caattaaaac agcagtggta
     2761 taaattcctc tattcaaggc atttaacaat tgtttttgtg aagcatcttc ttttgatctc
     2821 actaagaata ttcctaaaat agaaagaata attcctactg aggcgataat cattggagct
     2881 ataacaccat taaactgggt tgtggtacca aaaccagcaa aagcagtggc tcctaaggca
     2941 gccgtagaaa ggattgaacc acaataagat tcgtacaaat cggctcccat tccagccaca
     3001 tcaccaacgt tatctcctac attatcagca atagttgcag gattacgagg atcatcttca
     3061 ggaatattag cttctacttt acctacaaga tcagcaccta catcagcagc tttggtaaaa
     3121 attccaccac caacacgagc aaataaagat tgggtagaag cacccattcc aaaagttaag
     3181 gtagtagtag taataataat cagtttcatt gaaggatcgg ctgcaatagc aagatctgtt
     3241 gtgttgtcta aagcaccaat aaaaaattgt agcacataaa accaaataga aatatcaagc
     3301 aagccaagtc ccacaactac taaacccata actgctcctg agcgaaaggc gatttttaaa
     3361 ccactattca atgaatgttg tgcagcatta gctgctctgg cagaagcata agtggcagtt
     3421 ctcatgccta tatatccagc taaccctgaa aagaaaccac ctgttagaaa agcaaaaggc
     3481 acccatggat tttgtatatt gaatccataa gccataatag caaaaagaat ggtaagacac
     3541 aaaaaaacaa ttgcaactac tttatattgc tgctttaagt aggcaaaagc cccttctcgt
     3601 acatgtttag caatggtttt cattgttaca gttccttcgc ttgctttcat catttgatga
     3661 gaaaaatacc aagcaaatag tagtgctaaa actcctgctg ttggacacaa aaagaaaatg
     3721 ttattttcca taagaattta atttatgtat aagaaactaa tttagttaaa cgttgatata
     3781 aagttggatg agaataatga aaaaacacat ataatggatg tggcattaaa ttacttaatg
     3841 aagtttcaga taactttttt aatcctgatt gtaaaaaagt agcataacca tattgagctg
     3901 caaaagcatc tgcctgaaat tcaaattttc ttgataaaat attggaaaaa attcccaaca
     3961 acaatgaaag aggagcatat aacaagctaa acacaatcac attgatgtga aaagaagcag
     4021 atgcaactcc caagacttgt gcaaaaatat cacttttaag aatataaccg agcaaaaaaa
     4081 acaacaacaa atttacaggt aatgatatta aaaattgata aagagtgtgt ctgtgtttgt
     4141 aatgaccaat ttcgtgagca agaacagcaa caatttcttc tgtggttaat ttatttaaaa
     4201 gagtatcaaa taatacaatc cttttcttac ttcctaatcc actgaaataa gcattagctt
     4261 ttgtgcttcg tttcgaactg tcaataacat aaatattaga tagcttaaaa cctgcttttg
     4321 aggcaaattt ttcaatttca gttcttaatt ctccttctgg caaaggtgtt tgtttattaa
     4381 ataagggcac aataatttct gaataaaaca tattcataat taaactaaaa aatgttacca
     4441 ccaaccacgc ccataaccaa aaataattgg gtgttaatgt gtaaatccaa ataatagtgg
     4501 ctaaaatacc gccaccaatt ataacagaaa gcaaaatttc ctttatttta tcaacaataa
     4561 ataattttgg tgttacttta ttgaacccaa atttttcttc aatcacaaat gtatcatacc
     4621 attcgaaagg aagattaatt atttcactgg caaaaaataa aactccaaaa tataaaagag
     4681 gtaataaaat ttgatgttct gttatttttc ttaaaagatt atccaaccag ccgaagccac
     4741 caaaaataag catcagtaaa atgataacaa aacttaatgt gcttgttaac atgctaaaac
     4801 gggtattggt ccgaaaatat tcctgttgct ttttatattt ttcttcatcg taaatacctt
     4861 gaagaatttc aggaagctct tgcttggaat attggatatt aagataggtt aagtaacgtt
     4921 ctaaaacaaa gtcagctacg acaaaaaaaa taataagaaa aaacaatatt tttactatca
     4981 taacgaataa tgtgctttct aaaaattgac cacaaagata atgattttaa taaattcaaa
     5041 ctatcctttt ttatgaattt taataaataa gctatcattc atctatgttt caaaaagttt
     5101 tttgttcaat agttataata aaaatcactt tccccgactt ttttggaatg tgaagtatta
     5161 atgttgtgtg aggagctttt aagcagtaaa agaaatagtt atctcaatta atcagcaaat
     5221 taattggcta ttttcatttt ctttcgccaa aaatagaagt tcctaatcga accattgtac
     5281 taccttcttc tacagcgatt aagtagtcgt ttgacatacc catggaaagc tctgaaaaag
     5341 aatcattatt attaaaatat ttatttttaa gtaaatcgaa taattttttt aaacctctaa
     5401 attcatttct gatttgagtt atattgtctg taaaagttgc cattcccatc aatccacaaa
     5461 ttttgatatt agtatatttt tgccattcat cacttttaaa cagttctatc aattcatctg
     5521 gattaaaacc gaattttgtt tcttcttggg ctatgtggac ttgcagcaga caattaacag
     5581 ttttatttat tttttctgtc tgtttattaa tttcatttag caatttcaag gaatcaacac
     5641 tgtgaatcaa gtcaacaaaa ggcaatatat attttatttt attagtttgt aaatgaccaa
     5701 taaagtgcca ttgaatatct tttggtaaat tttcatattt gatacaaagt tcttgggtgc
     5761 ggctttctcc aaaaattctt tctcccgcgt cataagcttc cctgatagct gcttcaggct
     5821 gaaatttaga gatacaaacc agatgaacag acgaaggtaa ttgactatga atttctttaa
     5881 gattttcttt aattgacatg tcgtaatttt ttaattaaca gcaatttata agttttaaaa
     5941 attgatttct attctaatag agtaaaaata actgaaattt atcagccact tttaaaaagg
     6001 aaaaaactaa acaatgaaca aaattaatat attttctatg gtttatgcgg aaatagataa
     6061 ttatagaaag ttaaattttg tgatgttagt tatttcataa gttttggtta tttttgttgt
     6121 cgaggagcac ccaattatca ataatttttt taagtttcgc aatttgtcat gctttctatt
     6181 ttaatcccta cttataatta taatgttacc agtcttgtga aagaacttca caatcaagct
     6241 attaatcttt atattgattt tgaaattatt gtaatggaag atggctctca atattttgta
     6301 gaagaaaatg aaaatataaa taagttgaca aattgtcgac atattatttt agaggaaaat
     6361 attggcaggg cagcggtaag aaataaatta gccgaagaag caaaatacga tcatttatta
     6421 tttttggact gtgatgccaa aatttgttct gaacacttta ttgaaaaata cttggttttt
     6481 tgtaaagaga catcgcttgt tgtaattggt ggacgtattt atgaacaaga gaataatctg
     6541 gataacttta gtcttatgag aaaatatgga ataattaggg aacaaaatac tcttgagaat
     6601 cttaaaaata gaaaaaaata tccagtattg acaactccta attttctgat atcaaaagag
     6661 ctctttctaa aaacaaaatt tgatgaaata ttttcaggat atggtcatga agatacaatt
     6721 ttcgggatta aacttgttag aaatgaagca actttttttt acatcgataa tccagtagtt
     6781 catataggat tagaaactaa cgaaaaattt attaaaaaaa cagaaaatgg actaaaaaat
     6841 ttgtataata tctatctctc agcagattat cctgaaataa aacaactgtc aaaagtttta
     6901 tctatatatt taaaaatcaa taaaataaaa ttatcaaaga tattttgctt tatttacaaa
     6961 cattttaatc aaaatataaa aaaaagatta atttattctt taaatccttc cttatttttg
     7021 tttgattttt ataagctcct ttttctatgt gattattcct tcaagaatga caagatgaca
     7081 gagtaaggtt ttttgataac aaaaaatttt actgtcaaaa ctgacaacaa tgtcttgggc
     7141 acacattttg caaaaagatt tcacaaattg tttttctttt taaatgtatg tgtataaata
     7201 atattaataa taattgtatt tttattaacc atttaaaaat taaatagata tgaatattaa
     7261 accattagca gatcgtgtat tagttaaacc ttcaccggta gaagaaaaaa cttcaagtgg
     7321 aattattata ccggattcag caaaagaaaa accattaaaa ggagaagtaa ttgctgttgg
     7381 taacggaaca aaagatgaag aaatgacagt agccgttggc gatacggtat tatatgggaa
     7441 gtatgcaggg acagaattag aatgggatgg tgaaaaatat ctcattatgc gtcaatctga
     7501 tattttagca attattcttt aagaaaaaag ctaaactaaa aaatttcatt tttgaattat
     7561 atgctaacat aatgaattaa cattaaaatt ttaaaaatat ggcaaaagta attttatatg
     7621 atcttgaagc tcgtgaacaa ttaaaagaag gagttgacga attagctaat gcagtgaaag
     7681 taacattggg accaaaaggt agaaatgtaa ttatggaaaa gaaattcggt gctccagtca
     7741 taacaaaaga tggtgtgtcc gtagcaaaag aaatagaatt ggaagatcct tttaagaatg
     7801 ttggagctca attattgaag gaagtagctt caaaaaccag tgaagatgct ggagatggca
     7861 ccactactgc cacagtttta gctcaggcta ttgttaatgt aggtttaaaa aatgtaactg
     7921 ctggagcaaa tccaatggat ttaaaacgtg gaattgataa agcagtagct gaagtagtaa
     7981 aaagtatagc caaccaatca aaacaagtag gtgacgatta caataaaatt gaacaggtag
     8041 caactgtttc tgctaacaac gaccgtgaaa ttggtaaatt aattgccgat gcaatgcgta
     8101 aggtttcaaa agaaggtgtt attaccatag aagaggcaaa gggaacggaa acaactatcg
     8161 agatggtcga aggaatgcaa ttcgatcggg gttacatttc tgcttatttt gtcactaatg
     8221 ctgaaaaaat gcttgttgaa atggaaaagc cctacatttt aatccatgat aagaaaattt
     8281 ccaatttcaa agaattgctt cctatccttg aaccagttgc acagtcaggt cgtcctttgt
     8341 taattatagc tgaagatgtt gaaagcgaag ctcttactac tctggtagtt aatcgtcttc
     8401 gtggttcatt gaaagtttgt gctgttaagg ctccaggttt tggcgatcgt agaaaagaaa
     8461 tgctggaaga tattgctatt ttaacgggag gtgtagttat caccgaagaa agaggacttc
     8521 agcttgaaaa ggctacaatg gacatgttag gaacagcaga aaaaataaca gttgacaaag
     8581 agaatacgac aattgttaat ggagctggtt caaaagaagc tattcaagca cgcattaaac
     8641 aaattaaagc acaaattgcc aatgcaactt ctgattatga tcgtgaaaaa ctgcaagaac
     8701 gtttagctaa attagcggga ggtgtagcag tgttatatgt gggtgctcct tccgaaatag
     8761 aaatgaaaga gaaaaaagac cgagtagaag atgcattgag tgccacccgt gctgcaatag
     8821 aagaaggtat tattcctggt ggtggagttg cttatattcg tgctattgaa gctcttgaaa
     8881 aacttaaagg agaaaatgat gacgaaaata cgggtattga aattgtaaag cgtgcaatag
     8941 aagaaccact tcgtcaaatt gtggagaatg ctgggaaaga aggtgcagta attgtaaata
     9001 aaatcaagga agggaaagat gattttggct acaatgctcg tacagaaaac tttgagaatt
     9061 tctatgaaac cggagtattg gatccagcaa aagtggcacg tattgcactt gaaaatgctg
     9121 cttcgattgc aggcatgttc ttaactactg aatgtgtaat cactgagaaa aaagaacctg
     9181 cccctgttgt acagcaacca atgcctggca tgggcgatta tgaataataa aaatttttaa
     9241 aataaaaaaa ggcttctgga aaaattaata ctcagaagcc tttttttaat ttgttcttat
     9301 tttaaaacta acattcatct gcctgttggt aagggattac aaattctttg taacagaaga
     9361 tttttctcta cttcgtctac acttgttttt tcggcttgtc agacaacaac aatctttttg
     9421 cttttatgac tttgcatttt attttatttt atttacttgt tgctattgat tatcagctaa
     9481 aatattttca ctatcctaca atttcgtgat ctgtaaatca ccaactttaa acttttttct
     9541 aaattcattt ttcatttttg aaagctactt gatattaaaa aaaaatatta tataaatttg
     9601 tgaatattcc tttctcccat tttcttatta aaaaaacagc acagaatttg tcatataaaa
     9661 tgttaaatcc aaagagtttt tctatgtatt ttgaaagtta aattcagtga aagaattttt
     9721 tattaattta atttttttgt atatttgtat taaaatagca ggctggtttg tcgcttcttt
     9781 ttcaaaggag aagaggaaag tccgggcaac acagagcacc atacttccta acaggaagct
     9841 atctgcaaag gtagagaaac gtaacagaaa ataaccgtct catttctatg agataagggt
     9901 gaaaaggcga ggtaagagct caccggtatg attggtgaca atcatagccg tacgacttat
     9961 gggttgcaag atcaaatata ccgacgcatg tagggtggct cgtccgatgt cggggggtag
    10021 atcgctaaaa gcaaatggtg acatttgttt cagataaatg gcaaacgctt tgaaaaaagt
    10081 acagaacccg gcttacaagc ctgctatttt tt
//
LOCUS       DAOSAS010000059        10085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4380_length_10085_cov_11.949651, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000059 DAOSAS010000000
VERSION     DAOSAS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4380_length_10085_cov_11.949651"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLR72_04065"
     CDS             complement(<1..312)
                     /locus_tag="PLR72_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HOK36413.1"
                     /translation="MGKKVNKNENKQEEPLEKQPLGKLEATLWKAADKLRKNIDAAEY
                     KHVVLGLIFLKYISDAFEELYARLKAEEELGADPPVFRTFSGHPYNILIFNNNFIILG
                     VP"
     gene            946..1167
                     /locus_tag="PLR72_04070"
     CDS             946..1167
                     /locus_tag="PLR72_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5652 family protein"
                     /protein_id="HOK36414.1"
                     /translation="MCLPTNFLAEIVQYQLLLIVLLLWETVWKAIAMWKAARNNHLAW
                     YICLLIFNTVGVLPIIYILLKRKKETQAV"
     gene            complement(1230..1302)
                     /locus_tag="PLR72_04075"
     tRNA            complement(1230..1302)
                     /locus_tag="PLR72_04075"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Gly,seq:ccc)
     gene            complement(1385..1960)
                     /gene="rsxA"
                     /locus_tag="PLR72_04080"
     CDS             complement(1385..1960)
                     /gene="rsxA"
                     /locus_tag="PLR72_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOK36415.1"
                     /translation="MTYFLIFISAVFVNNIVLTQFLGICPFLGVSKKIDTALGMSGAV
                     VFVMTISTIITYLIQKGILAPFNLEFLQTIVYILVIAALVQLVEIILKKISPSLYQAL
                     GVFLPLITTNCTILGVAILVIQKEFNLMESVVFAISTAIGFGLALILFAGIREQLSMV
                     KLPKGMEGAPIALLTAGLLAMAFMGFAGIVK"
     gene            complement(2039..2638)
                     /locus_tag="PLR72_04085"
     CDS             complement(2039..2638)
                     /locus_tag="PLR72_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOK36416.1"
                     /translation="MSQSKFKIILNGLITENPIFVLILGMCPTLATSNSALNGISMGL
                     ATSFVLIGSNIVISMLKNVIPEKVRIPAYVVVIATFVSVVEMFMQAYVPALYESLGIF
                     IPLIVVNCIVLGRAEAFASKNSVLDSTLDGLGIGLGFTIALGILGSIRELLGTGKIFN
                     VNIFSDHYGTLIFILAPGAFIALAYLIAIVNKLIKHIKI"
     gene            complement(2655..3323)
                     /locus_tag="PLR72_04090"
     CDS             complement(2655..3323)
                     /locus_tag="PLR72_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOK36417.1"
                     /translation="MKKLESSLKNMVGVLLAITVISALTLSSVYSVTKDPIEKSKTVK
                     QQNAIKEVIPGFDHLATPEKISLNSGDTLTIYKAYDKDNVFIGAAVESFSKKGFGGEI
                     KVMVGFDKEGNIINYSVLEQKETPGLGTKMVDWFKTPIGNQDIRGKNPAKNNLTVKKD
                     GGEVDAITAATISSRAFLDAIHNAYAAYSRNFENKEENKIDSESGASKIEHQDSTKKD
                     SMNN"
     gene            complement(3320..4285)
                     /locus_tag="PLR72_04095"
     CDS             complement(3320..4285)
                     /locus_tag="PLR72_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOK36418.1"
                     /translation="MNNLVVSPAPHIHSGNSVPKEMKGVLIALIPAYLAALYYFGLGA
                     FIVSITAVISCVAFEYLIQRFLLKGELTINNLSAVVTGLLLSFNLPSNLPIWIIIIGS
                     LAAIGIGKMSFGGLGNNPFNPALIGRVFLLISFPVQMTTWPKPIPFNIAYLDAETSAT
                     ALTLLKEHTGTLPSTLDMFLGNIGGSLGEVSALALLIGFVYLLVKKIISWHIPISILA
                     TVAIFAGILHFIHPEMCASPLTHLLTGGLLLGAIYMATDYVTSPMSKLGMIIYGIGIG
                     LITVIIRVWGSYPEGMSFAILIMNGFTPLINAYVKPKRFAEVVKQ"
     gene            complement(4302..5630)
                     /gene="rsxC"
                     /locus_tag="PLR72_04100"
     CDS             complement(4302..5630)
                     /gene="rsxC"
                     /locus_tag="PLR72_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOK36419.1"
                     /translation="MKTFKIGGIYLEEYKLSAGERTKVITLPQQVVVPLRHYAGKPSI
                     PQVKRGDFLKVGQLVGLADGFVSANVHSPVSGKVAKIDNALDAYGYKYPSVFIDVIGD
                     DWLETIDLTPEVNHTCELTSSEIIKKIAESGIVGLGGATFPTHVKLTIPAGKKAEVLI
                     INGAECEPFLTCDHQLMLEKGEEIIVGVTILMKALNVKKALIGIECNKNDAIINMKKW
                     AKTEEGIEVVPLQTKYPQGCEKQLIEALTGRRVPRGGLPVDVGAVVQNVATTFAVYEA
                     VQKNKPLIERIITVTGKSMSKPGNFWVRIGTPLSHVVEQVGGIPEDTGKIVAGGPMMG
                     RALFSLEVAVAKGTSGLLFIPSAEAYRKKVENCIRCAKCVFSCPMGLEPYLLMNFGEK
                     MLWEEMEKNQVLDCIECGSCSFICPSHRPLLDYIKNGKVAINNIKINSNN"
     gene            complement(5675..6541)
                     /locus_tag="PLR72_04105"
     CDS             complement(5675..6541)
                     /locus_tag="PLR72_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HOK36420.1"
                     /translation="MNVILISVLSLGGIGALSALILYFVAQKFQIFEDPRIDEVEAVL
                     PAANCGGCGYPGCRAFATACVKAESLDNLVCPVGGAPVMEKVASILGKTAATITSTIA
                     VVRCGGSCEYRPRVNVYDGVKTCAIVHQLYQGETLCSWGCLGFGDCEAVCTFDAIHIN
                     PQTLLPEVDEEKCTSCGLCVKACPRNIIELRQKGPKSRRIYVNCVNQDKGAVNKKVCT
                     VACIGCSKCQQVCNYDAIIIANNLAYIDDEKCKLCRKCVDVCPTNSIVEINFPPRKTK
                     TETSSNISITEQ"
     gene            complement(6592..7014)
                     /locus_tag="PLR72_04110"
     CDS             complement(6592..7014)
                     /locus_tag="PLR72_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOK36421.1"
                     /translation="MSQSIEHSGIVEQIDGDQIKVLIMQQSACSECHAKSLCSIADKK
                     EKIIEVISSDPTLKVGDKVIIYGESSIGLQAVLLAFIIPFVLIFLALFIFQHFIENEL
                     IAGILAILVLVPYYLILFLSNKRLKNKFQFKIKKEDTI"
     gene            complement(7363..9402)
                     /locus_tag="PLR72_04115"
     CDS             complement(7363..9402)
                     /locus_tag="PLR72_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOK36422.1"
                     /translation="MKKLIFVLMTSFLIACSHQESQTPSIQDFKYSVDRFADIEILRY
                     RVLDFEELSLQQKELIYYLSQAALEGRDIIYDQNNKFNLCVRRTLEAIYTNYRGNRKS
                     GDFHQLTVYLKRVWMSNGIHHHYAEDKFIPEFSQVFFIEAVKSIDPSQLPLLKGETVD
                     DLLKKLIPVIFDPTVYPKGTNQAEGVDLILTSGNNYYEGVTQEEAEDFYNKMINPNDK
                     APISYGLNSKLVKENGIIHEKTYQINGLYGEAIKRIVYWLEKAAEVAENQQQREVINT
                     LIHYYRTGDLKIFNDYCIKWVQDTTSMVDFINGFIETYGDPLGLKGSWEALVDYKNLE
                     ATKRTEIISKNAQWFEDHSPIDKRFKKEQVKGISAKVVTAAMLGGDCYPSTPIGINLP
                     NADWIRHDYGSKSITIENITEAYDQAALKSGFNEEFMWSDIERERAKKYGSLTNNLHT
                     DLHECLGHGSGKLLHGVNPNRLKAYGSTIEEARADLFGLYFLSDPKMIELGLLPDNEA
                     YKAEYYQYMMNGLLTQLTRIQPGKNIEEAHMRNRQLIANWVYQKGLPEKVVELKKRNG
                     KTFVVVNNYEKLRSLFGMLLAEVQRVKSTGDYKAAKNLVENYGVKVDPVLHQEILERY
                     KKLNLAPYKGFVNPVYIPVIDKKGKITDVKISYDEDFVEQNLRYSKNYSHLPTFN"
     gene            complement(9429..>10085)
                     /locus_tag="PLR72_04120"
     CDS             complement(9429..>10085)
                     /locus_tag="PLR72_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOK36423.1"
                     /translation="WAKSFTLGLIFIITPGLLWFYSSSYKRQFLIGNLVIAFLSAICI
                     LLPSIMELAFLKIQYGNLIYQTPLPKKIYSWTGGFAFFAFLCTFIREIIKGMEDIEGD
                     KELECRTLPIVWGIQKTKNLIYLLIIFVIVSLMLTNNFLIPFQGTFTLKYIIFGLVIP
                     FFCLAFLIAKSKSQKDFHQTATFLKIIMLIGVLYSFVFYFLEAKEFNIPFFSVFISSQ
                     "
BASE COUNT         3428 a         1829 c         1493 g         3335 t
ORIGIN      
        1 tgggacaccc aaaattataa aattattatt gaatatcaat atgttatatg ggtgtccaga
       61 aaaagtgcgg aaaacaggag gatcggctcc cagctcttct tcggctttca gtcgggcata
      121 taattcttca aaagcatcag agatgtattt gaggaatatc aatcccaaca ccacatgttt
      181 atattcggca gcatcaatgt tctttctcag tttgtctgct gccttccaga gcgttgcctc
      241 gagtttgccg agcggttgtt tttctaaagg ttcttcttgt ttgttctcgt ttttgtttac
      301 tttcttgccc atatttttaa gatgttggcg attgtgctat cgcataattc gttgcagtcg
      361 atagtgtttc ttttgtaaat tcaacagttt tttgtttcaa attcaaaaat aaaaaatttt
      421 tataaattat caaacaaact tttaattttt gtaaaatcct tttgttaggt aatatatttt
      481 cttgtactat cgcttgttaa agtaaagtat tacaacttat tttgtttatt atgttatttt
      541 gagacgtatt atagttgcga actttatgtt aaaacggatt ttcctttcca aattttgtaa
      601 aaaatgaacc gaaagccaat accaatcagc ttttatgaac attttagtaa atttaatgta
      661 cgcaagaaaa atactatttt tttcttctaa ctaagttatt ttattgaatt tttttgtatg
      721 tttgtaaaaa ataaaataat gttcaatatg tgataaacaa ctaataatta cactgtaagc
      781 aagaaattat ttaaataaaa tattttaaac aaataataat caatttgttg tacataaaat
      841 atacctcctt tgaaaggaaa atccgttaaa aaaacatttt gttgaattaa aataatttta
      901 aacaaatcga ttttctaaaa atattcatct ttaaattcaa ccactatgtg tttacctact
      961 aattttttgg cagaaatagt ccaatatcaa ttactgttga tagttttgtt gctttgggaa
     1021 actgtatgga aagccattgc catgtggaaa gctgcaagga acaatcatct tgcatggtat
     1081 atttgcctgt tgattttcaa cactgttggt gttttgccta ttatctatat tttgctaaaa
     1141 agaaaaaaag aaactcaagc agtataaaac aaaaatctga aaaaaaaaga gactgccgtt
     1201 gtttctccag tagtctcttg aatatgctga gcggcaaacg ggattcgaac ccgcgacctt
     1261 tagcttggga agctaaagct ctaccgactg agctattgcc gcaaaaaaac tttttgcaaa
     1321 tgtaaaacga tatttaaaaa tagacaaatc aaaaaagaaa aatttaaacg cttattgaaa
     1381 aaaatcattt tacaattcct gcaaaaccca taaaagccat tgccagcaaa ccagcggtta
     1441 ataaagcgat tggcgcacct tccattcctt tgggtaattt taccatgctg agttgctcac
     1501 gaatgccagc aaataagata agagctagac caaaaccaat ggctgtcgaa atagcaaaca
     1561 ccacactttc cattaaatta aattctttct gaattaccaa aatggcaaca cctaaaatgg
     1621 tacaattcgt agttatcaag gggagaaata ctcctaaagc ttgatacaaa gatggtgaaa
     1681 ttttctttaa aattatttca accaattgca ccagagcagc aatcacaaga atgtaaacaa
     1741 tagtttgtaa aaattcaagg ttaaaaggtg ctaaaatccc cttttgaata agataagtaa
     1801 tgatggtaga aattgtcata acaaaaacta ctgctccact cattcctaag gctgtatcaa
     1861 tttttttaga tacacctaaa aagggacaaa ttcccaaaaa ctgtgtcagt acgatattat
     1921 ttacaaaaac agcactgata aatatcaaaa aataggtcat tttatttgta atttatccgt
     1981 tattaattaa ttttttgatt gataaataat aattgcatta tttgaaatga ttttctgtct
     2041 aaattttaat atgtttaatt agtttgttga caatggcaat caggtaagcc agtgcaataa
     2101 aggcacctgg agctaaaata aaaatcaaag ttccataatg gtctgaaaaa atattgacat
     2161 taaaaatttt ccctgtccct aacaattcac gaatgcttcc cagaatgccc aatgcaattg
     2221 taaagcctaa tccaatgcca agtccatcca acgtagaatc taaaacagaa ttttttgagg
     2281 caaaagcctc tgctcttcct aacacaatac aattaaccac aattaatgga ataaaaattc
     2341 ccaaactttc gtataaagca ggaacataag cctgcatgaa catttccacc acactgacaa
     2401 aagtagcaat caccaccaca taagctggaa tgcgaacttt ttcgggaatc acgtttttca
     2461 acatagaaat cactatattg gaaccaatta atacaaatga agtagcaagt cccatgctga
     2521 ttccatttaa agctgaatta gaggtagcta acgtggggca cattcccaaa atcaacacaa
     2581 atatgggatt ttccgtgatt aaaccgttca ggatgatttt aaatttagac tgactcattt
     2641 tttatcattt tttattaatt gttcattgaa tcttttttgg tagaatcctg atgttctatt
     2701 tttgaagctc cactttcaga atcaatttta ttttcttctt tgttttcaaa atttcttgaa
     2761 taagcagcat atgcattatg aatagcatcc aaaaatgcac gggagctaat agttgcagcc
     2821 gtaatggcat caacttcacc gccatctttt ttaacggtaa gattattttt tgctggattt
     2881 tttcctctaa tgtcttgatt gccaattgga gttttaaacc agtccaccat ttttgttccc
     2941 aagccaggag tttctttttg ttctaacact gaataattta tgatatttcc ttctttatca
     3001 aaacctacca taacttttat ttctcctcca aatccttttt ttgaaaaaga ttcaacagcg
     3061 gctccaataa agacattatc cttatcatag gctttataaa tagttaatgt gtcgccgcta
     3121 tttaagctaa ttttttcagg agttgctaaa tggtcaaaac ctggaattac ttctttaata
     3181 gcattttgtt gctttactgt ttttgatttt tcaatcggat ctttagttac tgaataaacg
     3241 gaacttaaag tgagagctga aattactgtg attgccagca acacaccgac catatttttg
     3301 agacttgatt ctaatttttt cattgtttaa ctacctccgc aaaacgtttt ggtttaacat
     3361 aagcattgat aagtggtgta aaaccattca taatcaaaat agcaaatgac attccttcag
     3421 gataagaacc ccaaacccga ataataacag ttattagccc tattccaata ccataaatta
     3481 tcattcctaa ttttgacata ggagaagtaa cataatcggt tgccatataa atagctccca
     3541 acaataaacc tcctgtcaaa aggtgagtaa gcggtgaagc acacatttca ggatgaataa
     3601 aatgaagaat tcctgcaaaa atagccacag tagccaatat gctgattgga atatgccagc
     3661 taataatttt tttcaccagc aaataaacaa aacctatcaa caaagccagt gctgaaactt
     3721 cgcctaacga accacctata ttcccgagaa acatatccag cgttgaagga agtgttccag
     3781 tatgttcttt taacaatgta agtgctgtag ctgaagtttc tgcatccaaa taagctatat
     3841 taaatggaat aggtttaggc caggtagtca tttgtactgg aaaagaaatt aacaaaaata
     3901 cacgtcctat caatgctgga ttaaagggat tgttccccaa acccccaaag ctcatttttc
     3961 ctattccgat agcagctaat gaacctatta taattatcca gataggaaga ttggaaggta
     4021 aattaaaaga aagcaacaat ccagtcacta ctgctgaaag attattaata gtcaattcac
     4081 ctttcaacaa aaatcgttga atgagatatt caaaagccac acatgaaatc actgcggtaa
     4141 tacttacaat aaaagcaccc agaccaaaat aatacaaagc agctaaataa gctggaatta
     4201 atgcaattaa tacacctttc atttctttag gaacggaatt cccgctatga atatgaggtg
     4261 ctggcgaaac aactaaatta ttcatatttg aattctatat tttaattatt gctatttatt
     4321 tttatattgt taattgcaac tttaccattt ttgatataat ccaataaagg acgatgagaa
     4381 ggacaaataa aactacagga gccacattca atacaatcaa gaacctggtt tttttccatt
     4441 tcttcccaca acattttttc cccaaaattc attaataaat aaggctccaa ccccatcgga
     4501 caggaaaaaa cacattttgc acagcgaata caattttcca ctttctttct ataagcttca
     4561 gccgaaggga tgaaaagcaa gcctgatgtg cctttagcaa cagctacttc cagcgaaaat
     4621 aaagcacgtc ccatcatcgg tccacctgct acaatttttc ctgtatcttc tggaataccg
     4681 ccaacttgct caactacgtg acttaatgga gttccgattc gtacccaaaa atttcctggt
     4741 ttcgacatcg attttcctgt aactgtaatt attctctcta tcaatggttt gtttttctga
     4801 acggcttcat agacagcaaa agttgtagct acattttgaa ctactgctcc aacatccaca
     4861 ggaagacctc ctctgggaac tcgacgtcct gtcaaggctt caattaactg tttctcacaa
     4921 ccttgaggat attttgtttg taggggaact acttcaattc cttcttctgt ttttgcccat
     4981 tttttcatat taatgattgc atcattttta ttacattcaa ttccaatgag ggctttttta
     5041 acattaagag ctttcattaa aatggtgaca ccaacaatga tttcttcacc tttttcaagc
     5101 attaactgat gatcgcaagt aaggaaaggt tcacattctg cgccattaat aataagcact
     5161 tccgcttttt tcccagcagg tattgtaagt tttacatggg taggaaaagt agcgccaccc
     5221 aatccaacaa ttccagattc cgcaattttt tttatgattt cagaagaagt caattcacat
     5281 gtatgattta cctcaggggt taaatcaatg gtttcaagcc aatcatcgcc aattacatca
     5341 ataaatacgg atggatattt gtagccatac gcatctaaag cattatcaat tttcgcaact
     5401 ttacctgaaa ccggcgaatg cacatttgca gacacaaagc catcagctaa acctactaat
     5461 tgtcctactt ttaaaaaatc gcctcgtttt acttgtggaa tactgggctt gcctgcataa
     5521 tgtcgtaaag gcactaccac ttgttgaggt aatgtaataa ctttggttct ttcccctgct
     5581 gataatttat actcttctag ataaattcct cctattttga aagttttcat tggatagtta
     5641 agtaaatttt atttttcttg taaaaattga aaaattattg ttctgtaata gaaatattgg
     5701 aagatgtttc tgtttttgtt ttgcgtggag gaaaatttat ttccactatt gaattagtag
     5761 ggcaaacatc cacgcatttt ctacacaatt tacatttttc gtcatcaata taagcaaggt
     5821 tgtttgctat gataatagca tcataattac aaacttgttg acattttgaa caaccaatgc
     5881 aagcaaccgt acatactttt ttattaacag ctcctttatc ttgattaaca caatttacgt
     5941 aaattcttct tgatttggga cctttctgac gaagctcaat aatatttcgc ggacaagctt
     6001 ttacgcaaag tccacatgaa gtacattttt cttcatcaac ttcaggtaac agggtctgtg
     6061 gattgatatg aatggcatca aaagtacaaa cagcttcaca atcgccaaaa cccagacaac
     6121 cccaactaca aagtgtttct ccctgataaa gttgatgaac aatggcgcaa gtttttactc
     6181 cgtcataaac attgacacga ggacgatatt cacaactccc accacatcgc actacagcaa
     6241 tcgttgaagt gatggttgct gctgtttttc ctaaaataga ggctactttt tccattacgg
     6301 gagctccacc aactggacaa accaaattat ctaacgactc agctttaaca caagcagttg
     6361 caaaagctcg acatcctgga taaccacagc ctccgcaatt agcggcaggt aaaactgctt
     6421 caacttcgtc aattcgcgga tcttcgaaaa tctgaaattt ttgtgccaca aaatataaaa
     6481 tcagagccga tagtgccccg attcctccta aagaaagtac tgaaattaat attacgttca
     6541 ttttgattta tctcttgagt ttaaagttgt tcaaaaaaat tttttacttc cttaaattgt
     6601 atcttctttt ttaattttaa attgaaactt gttttttaat cgtttattcg ataaaaaaag
     6661 aataagataa taaggaacga gcaccagaat ggctaaaatt cctgctatta attcattttc
     6721 aatgaaatgt tgaaatataa acaaagctaa aaaaattaat acgaaaggta tgatgaaagc
     6781 caataatact gcttgaagtc ctattgaact ttctccataa ataataactt tgtcacccac
     6841 ttttaaagta ggatcagaac tgattacttc aataattttt tcttttttat cagcaataga
     6901 gcataaactt ttagcatgac attcgctgca tgccgattgc tgcataatca gtacttttat
     6961 ttgatcgcca tcaatttgtt caacaatacc tgaatgttct attgattgac tcattttgta
     7021 atgtagacat tgcaattttt gagcacaaag gtactgatta tttattaatg actaaaaaaa
     7081 tattatagaa aatcattaac aaaaaagatg gaaaaaattg ttaatcagtt gtaatggtag
     7141 ataatatatt taatagcaga aacggatttt ttaaaaaaaa ttgtacaaaa tattacttaa
     7201 agttattctt aattatgatt gttgaatgac acactaaaaa tttaaatgaa taacctcgag
     7261 gaagcctcga ggtattttgt tagtcgaaca aaaaatttta tcaagcaatt ccttctaaag
     7321 gaataacaat aattttttat tctttcctac ttattatcaa attcaattga atgttggaag
     7381 gtgagaataa tttttcgagt agcgtaaatt ctgttctaca aaatcttcat cgtaagaaat
     7441 tttgacatct gttatttttc cttttttgtc aataacagga atatagactg gattaacaaa
     7501 tcctttataa ggagcaagat tgagtttctt gtaacgttcc aaaatttcct gatgcaaaac
     7561 aggatctact ttaacgccat aattttctac cagatttttt gctgccttgt aatcaccagt
     7621 agattttact ctttggactt cagccaataa catcccaaaa agtgaacgta atttttcata
     7681 attattaacg actacaaaag tttttccatt tctctttttc aattctacta ccttttcagg
     7741 caaacctttc tgatacaccc aattggcaat taattggcga ttacgcatgt gagcttcttc
     7801 aatgtttttt cctggctgaa tacgcgtaag ttgtgtaagt aagccattca tcatatattg
     7861 ataatattct gctttatagg cttcgttatc aggtaataag ccgagctcta tcatttttgg
     7921 atcggataga aaataaagtc caaacaaatc tgctcgagct tcctctatag ttgaaccata
     7981 agcttttaat cgattgggat taacaccatg caaaagtttt cctgacccat gtcccaaaca
     8041 ttcatgtaaa tcagtatgca aattattggt caaagaacca tacttttttg ctcgttcgcg
     8101 ttctatatca ctccacataa attcttcatt gaaaccgctt ttcaaagcag cttggtcgta
     8161 agcttccgtt atattttcaa tcgtaataga ttttgaacca taatcatgac gaatccaatc
     8221 ggcattaggc agattaatac caatgggtgt agaagggtaa caatcgccac ctaacattgc
     8281 tgctgttact acttttgctg aaatgccttt tacctgttct tttttaaaac gtttgtcaat
     8341 aggtgaatga tcttcaaacc attgggcgtt tttactgatg atttctgtac gttttgttgc
     8401 ttccaaattc ttataatcaa ccagagcttc ccaactacct tttaatccta atggatcgcc
     8461 ataagtttct atgaaaccat tgataaaatc aaccattgat gtggtatctt gcacccattt
     8521 tatacaataa tcattgaaaa tttttaaatc acctgttcga taataatgaa tcaaggtatt
     8581 aattacctct ctctgttgtt gattttcagc aacttctgct gctttttcaa gccagtaaac
     8641 aatacgcttt attgcttcgc catataaacc atttatctga taagtctttt catgaataat
     8701 accattttct tttactaatt tgctgtttag tccataagaa atgggagctt tatcgtttgg
     8761 attgatcatt ttattgtaaa aatcttcagc ttcttcttgg gttacacctt cataataatt
     8821 atttcctgaa gtaagaatca aatctactcc ttcagcttga ttggtacctt ttggataaac
     8881 ggttggatca aaaataacgg gaattaattt tttaagcaaa tcatcaacgg tttcgccttt
     8941 tagcaaaggt aattgtgaag gatcaatact ttttactgct tcaataaaaa aaacctgcga
     9001 aaattcagga ataaatttat cttcagcata atgatgatga atgccattgc tcatccagac
     9061 acgtttcaaa taaacagtta attgatgaaa atctcctgac tttctatttc cacgataatt
     9121 tgtataaata gcttccagag ttcgtctaac gcataaatta aatttgttgt tctgatcgta
     9181 aataatatct ctaccttcaa gagcagcttg actaagataa taaatcaatt ctttttgttg
     9241 aagagaaagt tcttcaaaat caagaacacg ataacgtaaa atttcaatat ctgcaaatcg
     9301 atcaactgaa tatttaaaat cctgaatcga aggagtttgt gattcctgat ggctacaagc
     9361 aattagaaaa gaagtcataa gcacaaaaat taattttttc atgttgataa ttaattagtt
     9421 gcgttagttt attgagaaga aataaacaca ctaaaaaaag gaatattaaa ttctttggct
     9481 tcaagaaaat aaaatacaaa actatataga acgccaatca gcataataat ttttaaaaaa
     9541 gtagcagttt gatgaaaatc tttttggctt ttcgattttg caattaaaaa tgccagacag
     9601 aaaaaaggaa ttaccagccc gaaaataata tatttcaacg taaaagttcc ctgaaagggg
     9661 attagaaaat tattggttaa cattaagctg acaataacaa agataatgag aagataaatc
     9721 aaatttttcg ttttctgaat accccaaaca ataggtaaag ttctgcattc cagctcttta
     9781 tcaccttcta tgtcttccat accttttata atttcgcgaa tgaaagtaca aagaaaagca
     9841 aaaaaggcaa atccgcctgt ccagctataa atttttttag gaagtggcgt ttgataaatc
     9901 aaattaccat attgtatttt aagaaaagcc aactccataa tagaaggcag caaaatgcaa
     9961 atagctgaaa gaaaagctat taccaaattt ccaattaaaa actgacgttt atagctagac
    10021 gaataaaacc aaagcaaacc tggcgtaatg ataaaaatga gtcccagcgt aaaactttta
    10081 gccca
//
LOCUS       DAOSAS010000060         9961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4454_length_9961_cov_11.691904, whole genome shotgun sequence.
ACCESSION   DAOSAS010000060 DAOSAS010000000
VERSION     DAOSAS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9961
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4454_length_9961_cov_11.691904"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1138)
                     /locus_tag="PLR72_04125"
     CDS             complement(8..1138)
                     /locus_tag="PLR72_04125"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOK36424.1"
                     /translation="MQFRDLKKQYEVLKKDIDQAMLEVAASGAFIMGKQVKELEQQLA
                     EYVGVKHCVSCANGTEALTLALKAWGIKSGDAVFVPDFTFFASAEVVSLEGATPVFVD
                     VDADTFNIDPVALEKTIEKTIKEGKLQPKVIITVDLFGLPADYPEIRKIADKYHLLIL
                     EDGAQGFGGEINGKKACSFGDISTTSFFPAKPLGCYGDGGAIFTDNDEWASLMDSLRV
                     HGKGSFKYDNVRIGMNSRLDTIQAAILKVKFDAFKKYELNDVNKAADLYTKKLKDLVK
                     TPVVPEGYLSSWAQYTIQLQAEKQRDGLQAYLKEQGIPTMVYYPKPMHEQTAYLNLGY
                     HSGDFPATESLCGKVLSLPMHPYLTEEEINKVCQAISEFLVR"
     gene            complement(1408..>1734)
                     /locus_tag="PLR72_04130"
     CDS             complement(1408..>1734)
                     /locus_tag="PLR72_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOK36425.1"
                     /translation="PSCVFTNVKTPRAHYPVHGIYDKTLIKNGASLGANCTVVCGHTV
                     GRSAMVAAGAVVTKNVKDYALVAGVPARQIGWVCECGERLEQNLVCKKCGKKYQETEN
                     GLKEKV"
     assembly_gap    1735..1834
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2221..2811
                     /gene="ruvA"
                     /locus_tag="PLR72_04135"
     CDS             2221..2811
                     /gene="ruvA"
                     /locus_tag="PLR72_04135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOK36426.1"
                     /translation="MIDYIKGEIVELTPTYVVIETGGIGYFINIALPVFSLLSGKNSV
                     KLYIYEVIREDAYLLYGFSTKDERQLFLLLISVSGIGANTARMIMSSYSVEELQGIIA
                     SENDSALSSIKGIGTKTAQRIIVDLKDKILKITSNKENLPAFKDTTSQIKEEAIAALI
                     MLGFSAAVSQKAIDSILKKQSVANVEDLIKQVLKII"
     assembly_gap    2933..3032
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3033..>6694
                     /gene="sprA"
                     /locus_tag="PLR72_04140"
     CDS             <3033..>6694
                     /gene="sprA"
                     /locus_tag="PLR72_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOK36427.1"
                     /translation="TYVGEIEIATPFLMTEKEYYDYTLKKEMENYWREKNVGSQIDNE
                     DKFRLTDMKFDIGPAEKVFGPGGVQVRTQGSAEILFGYKTNKIDNPALTERMRKTGIF
                     DFEEKIQMNVNGKVGDKVNFNLNYNTESSFEFDQKMLKLNYKGKEDDIIKSIEAGNVS
                     MPLNSSLIPGSSALFGIKTDLQFGKLSVSAIASQQEAETKTISTRGGAQTTKYEINID
                     NYDENRHFFLSHYFRNNYENAMSKLPYVMSGITITRMEVWITNKRGNYEQARNIIAFE
                     DLGETDRIDNPHWQPTSTNLLPYNKANSLYDEIVSLPNVRDIQQTNNILSSQYGALGI
                     VGGQDYEKIESARKLDPSEYTFNPTLGYISLRNMLQPDEVLAVAFEYTYGGQTYQVGE
                     FSTDPITAPNALIVKLLKSTTQSPSLPSWDLMMKNVYSLGATQIQPENFILEIVYRND
                     SVGTELRYITEGNIKNKLLLQVMNLDRLNSKNAPQPDGKFDYVEGYTIFSSSGKIFFP
                     VLEPFGSHLKQAIGNDQIAEKYIFQELYDSTLVMAQEFSEKNKFRIIGEYKASSRNEI
                     RLNAMNIPRGSVVVTAGGKTLVENVDYTVDYIMGTVNIINTSLLESGTNIDVKLENRS
                     MFNMQRKTLLGTHLEYQFNRDFSLGGTIMHLSEMPLTTKVNTGNEPISNTIWGLNTSW
                     RKESQWLTNQLDKLPFVNATKPSTIALNAEVAQLIPGHSSVISAAGLAYIDDFESTQT
                     TIDIHYPAFWYLASTPYNPNGGLFPEAALSNNVEYGKNRALLAWYYVDPILNANSRNT
                     PPNLRNNVESQSNHLTRNVLEEEIFPNKERLATQTSRLTILNLSYYPTERGPYNLDAD
                     NINPDGTLMNPQQRWGGIMRKLDVTDFETANIEYIEFWMMDPFIMDKDGTQRGGDLYF
                     NLGDISEDILKDGKKFFEQGLPLDADITKVDTTVWGKIPKTQSTVKAFDNSPGARAKQ
                     DVGLDGLPTNEEQQFPTYKNYVEKINQKIDGETRQKWQSDPFSPLNDPAGDNYHFYRG
                     SDYDAQEKDILSRYKYYNGTEGNSPEAENTQENYSTSATSLPDGEDLNGDNTMNEYEK
                     YYQYHVQIKREAMEVGRNYITDKIVSNVKLENGKVEPVTWYQFKIPIREYDEKIGNIR
                     NFKSIRFIRLFLTNFEQETHLRFATLDLVRGEWRSYTKSLFPAGSTPISEGKLDVHSV
                     NIEEDADKTPVNYILPP"
     assembly_gap    6695..6794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6795..>9961
                     /gene="sprA"
                     /locus_tag="PLR72_04145"
     CDS             <6795..>9961
                     /gene="sprA"
                     /locus_tag="PLR72_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOK36428.1"
                     /translation="NYYEYEVPLRVTPHGKYLNEKNEDREIVWPLENMFDFPFSTLIE
                     AKLKRDKYLQTSGGNAPTLTPYEVYDPDNPKNKIKIVGNPSISDIENIMIGIRNVSGE
                     IKSGEIWVNELRMSKFNESGGWAAMGNLAVGLSDIGSLNFSGRIETAGYGSLESNVMN
                     RRLEDLRQMNFSTALEVGRFLPEKAKLQIPLYFSYTNETVSPKYNPLNQDVELKDALE
                     NLTSKTERDSLLNLSQEVNTSKSFNISNARINIRSKKPQFYDPANWRFTYAYIESDKY
                     TPEIEQDMNKSQRAAIDYSFNFNPQPWEPFKNIKSLNKPALKIINDFNIYYLPSSINF
                     NTNLNRQYSQTKLRALEVSSVGTSDKLENLSFSKDFMFNRQFEIKYDLTRAMKFSLQA
                     AMNANIDEPYFTPEIGKEYYEAWRDTVWYNIRKLGTPYAYQQVFTASWNIPINKIPIT
                     SWIDANISYNSNYNWNRSALLEKDGEGLGNIATSIGSWQTNGQFNFEGLYRKSNFLKN
                     VLNRNSSFATSQRQTFKPREYKQIVNLSNNQTLTVRHNLGSESVRLKAIDKNGNTIPL
                     QYKVRSNSTVDIFPKQNLDSVQLMLTTISPNERSVSGQVVDFTLGALMMVRRVSFTYR
                     ESNSMVLTGFLPEAGFLGQKKYNGFNAPGYPFTFGIHDDNMIQKAIEKGWLNTGDSIV
                     NPATTAFTSDFDLKASLEPIPGFKIDLNATRYTASNTSVLFMYEGMPTTYTGSFNITQ
                     IALSTAFKKIGSVENNYNSSTFNMFLQNRQKIADRLNSKYMGTNYPAAGFITSTNLAG
                     KKFDPSVGIFSLNSPDVLIPAFLAAYTGKDASNVNTSPFISLLNILPNWRISYDGLSR
                     IGWVKNHFKNINLTHGYTCRYSVGSYSSYSTWVPMSEDETFGYIRDVQTSLPIPSSAY
                     DISSVSINEQFSPLLGINTTMNNSMTTKLEYRKQRNLSLNVSSAQLIESLSDEYVIGF
                     GYVLKDFDVILKLKTDKQSRIKNDLKLSLDLSYRDIKTLLRKIEENITQASSGNKLFT
                     LKFLADYVFSSKLNIQLFYDRQSSTP"
BASE COUNT         3276 a         1642 c         1687 g         3056 t
ORIGIN      
        1 ccgaaactta tctcaccaag aactctgaaa ttgcctgaca aaccttattg atttcttctt
       61 ctgtcaaata gggatgcatg ggcaaggaaa gcactttacc gcaaagagat tcagtagccg
      121 gaaaatcgcc ggaatgataa cccaaattga gatacgctgt ctgttcgtgc atgggtttgg
      181 gataataaac cattgttgga atgccctgct ctttcaaata agcctgcaaa ccatctcgct
      241 gcttttcagc ctgcaattga atggtatatt gtgcccaact ggaaagataa ccttcaggaa
      301 caacaggcgt ttttactaaa tctttcaatt ttttcgtata aaggtcggct gctttattga
      361 catcgttcaa ttcatatttt ttgaaagcat caaatttaac tttcaaaatg gcagcctgga
      421 ttgtatcaag acgtgaattc attccaatgc gtacgttatc gtatttgaaa gaacctttgc
      481 catgaacacg caaggaatcc atcagcgaag cccattcgtc gttatcggta aaaattgctc
      541 cgccatcgcc gtaacaaccc agcggtttgg cagggaaaaa cgaagtagta gaaatatcac
      601 caaaactgca cgcttttttg ccgttaattt caccaccaaa accctgtgct ccgtcttcca
      661 gaatcaacag atgatattta tcggcaattt ttcgaatttc cggataatcg gcaggaagcc
      721 caaacaaatc aactgtaata ataactttag gttggagttt gccttccttg atggtttttt
      781 caatagtttt ttccagtgca acaggatcga tattgaacgt atcggcatcc acatccacaa
      841 atacgggtgt tgccccttcg agcgaaacga cttcggcgga agcaaagaaa gtaaaatcgg
      901 gaacaaaaac tgcatcaccg gatttgattc cccaagcttt taatgccagc gtcaaagctt
      961 ccgtaccatt ggcgcatgaa acgcagtgtt tcacaccaac atattctgcc aactgctgtt
     1021 ccaactcttt aacttgtttt cccataataa atgcaccgga agcagccact tcgagcattg
     1081 cctgatcaat gtcttttttc aggacttcgt actgtttttt taaatcacga aattgcatac
     1141 ttttttattt agttttgaat tttacaaaat ttacaaagtg ccaacggtat tttcaaaata
     1201 cgcaaaaaat tagcgttcat gagcgtgaaa aaatacatct ctcgcagatt attgcagatg
     1261 aatacgcaaa tagacgtaga gaatcagcga aagaaatcag cgtaaatcag cgagaaaaaa
     1321 tatttcacaa gaacttttaa taacaggaaa aaattcctaa ttaaattcta aactttttta
     1381 cacttcgtaa tctggatttt gttccattta aactttttct ttcaaaccat tttctgtttc
     1441 ctgatatttt ttcccgcatt ttttgcagac taaattttgc tccagtcgtt cgccacattc
     1501 gcatacccag ccaatttgtc gagcagggac acctgcaacg agggcataat cttttacatt
     1561 tttggtgacg acagctccag cagctaccat agctgaacga ccaactgtat gtccacaaac
     1621 gaccgtacaa tttgctccca gtgaagctcc atttttaatt aacgttttgt cgtaaatgcc
     1681 atgaacagga taatgagcgc gaggtgtttt tacgttggta aacacacagg atggnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgtatc gattctatct ggcatcatct
     1861 tatatattct aaaagtttta caaagataag attttttaat aaaaaaatgg tcaacgttat
     1921 ttaaatattt acctttcttc ctccaccctc tcatacttcc attcttctct ccgtctgtaa
     1981 actcaaaatc ctaatttaca actattttta aattattttt tattttcgaa agctatttaa
     2041 gaatagaaaa attgtacatt ttcatttttt aactgaaatt ttttcttttc tttttaattg
     2101 cttaatcgta attttttata gaaatcaacg ttaactgaaa ataaggtttt ttattctatg
     2161 acttggtaaa gttggtatat ttccagataa attcttattt ttacaccaaa ttttaaccag
     2221 atgattgatt atataaaagg tgaaattgta gaattaacac ctacttatgt ggtgattgaa
     2281 acaggaggaa taggctattt tatcaatatt gctctccctg tgttttcttt attaagtgga
     2341 aaaaactcag ttaagttata tatttatgag gtgattcgtg aagatgctta tctactttat
     2401 ggctttagca caaaagatga aaggcagctt tttttgttat tgatctctgt ttcaggaatc
     2461 ggagcaaata ccgcacgaat gattatgtcg tcttattccg ttgaagagct tcaagggata
     2521 atagccagtg agaatgattc tgctcttagc tctataaaag gaattggtac caaaacagct
     2581 caacgaatca ttgtggactt aaaagataaa attttaaaaa ttacttcaaa taaagaaaat
     2641 cttcctgcat ttaaggacac aacctcccaa ataaaagaag aagctatcgc tgcccttatt
     2701 atgcttggtt tttctgcggc ggtttctcaa aaagcaattg attctatatt gaaaaaacaa
     2761 tctgttgcca acgtagaaga tttaattaaa caggttttga aaataatctg acttttaaag
     2821 cacattgata aatgggtaat ttctacttga tttaatctaa tagctttcta caaaagtgaa
     2881 aaacaattta tgcgaagggt gaaaaaatcg gtacgtgttt ttgtattttt cannnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacttatgt cggcgaaatt gaaattgcta
     3061 ctccttttct aatgactgaa aaggaatatt atgactacac tttgaaaaaa gaaatggaaa
     3121 attattggcg tgaaaaaaat gttggtagtc agatagataa tgaagataaa tttcgtttga
     3181 ccgacatgaa atttgacatt ggacctgctg aaaaagtttt tggtcccggc ggtgtacaag
     3241 ttagaacaca aggctctgct gaaattttat ttggttacaa aaccaataaa atagataatc
     3301 cagctttgac tgaaagaatg agaaaaacag gcatattcga ttttgaagaa aaaattcaga
     3361 tgaatgtaaa tggtaaagta ggcgacaaag tcaattttaa tttgaattat aatactgaat
     3421 cgtcatttga atttgaccag aaaatgctta aactcaatta taaaggaaaa gaagatgata
     3481 ttattaaaag cattgaagca ggtaatgtaa gtatgccctt gaatagttct cttataccag
     3541 gaagctcagc tttgtttgga ataaaaaccg atttgcagtt cggaaaatta agtgtcagtg
     3601 ctattgcttc tcaacaagaa gcggaaacca aaaccataag cacacgagga ggtgctcaaa
     3661 ctaccaaata tgaaattaat attgataatt atgatgaaaa ccggcatttc tttttaagtc
     3721 attatttcag aaataattat gaaaatgcaa tgagtaaact tccgtatgtg atgtctggca
     3781 ttactattac aagaatggaa gtttggatca ccaataaacg aggaaattat gaacaagcaa
     3841 gaaatattat tgcttttgaa gacctgggag aaaccgatcg aattgataat ccccactggc
     3901 aaccaacgtc aaccaatctt ttaccttaca acaaagcaaa ttcattatat gatgaaattg
     3961 tatctttgcc aaatgttcgt gatattcagc aaactaacaa tattctttct tcacaatatg
     4021 gagcgttggg aatagtagga ggacaagatt atgaaaaaat tgaaagtgct cgtaaactgg
     4081 atccctctga atatactttt aatcctacct taggttatat ttctttacgc aatatgttac
     4141 aacctgatga agtgctggca gtggcttttg aatatactta tggcgggcaa acttatcaag
     4201 taggtgaatt ttcaaccgat cctataactg caccaaatgc attgattgtc aaattattaa
     4261 aaagcactac tcaatctcct tctttgcctt cgtgggattt aatgatgaaa aacgtttaca
     4321 gtttaggagc aactcagatt caacctgaaa attttatatt ggaaatagtt tatcgaaacg
     4381 attcggttgg tacagaactt cgatatatta ccgaaggaaa tattaaaaat aaattattac
     4441 tacaggtaat gaatcttgat aggctgaact ccaaaaatgc acctcaacct gatggtaagt
     4501 ttgattatgt agaaggatat actatttttt cttccagtgg aaaaatcttt ttccctgtgc
     4561 tggaaccatt tggctctcat ttaaaacaag caattggcaa tgatcagatt gcagagaaat
     4621 atatttttca agaattatat gattctaccc ttgtaatggc tcaggaattc agtgaaaaaa
     4681 ataaatttag aatcattgga gaatacaaag caagttcaag aaatgaaatc aggttaaatg
     4741 cgatgaatat tccacgagga tcggtagtag taaccgctgg aggaaaaaca ttggtggaaa
     4801 atgttgatta cactgttgat tatattatgg ggactgtcaa tattatcaac acttctcttc
     4861 tggaatcagg aaccaatatt gatgtcaaac ttgagaatcg ttccatgttt aatatgcagc
     4921 gaaaaacgct gttgggcact catctggaat atcaatttaa tcgtgatttt agtttaggtg
     4981 gaaccattat gcatctttcc gaaatgcctt tgacaaccaa agtaaatact ggcaatgaac
     5041 ccatttccaa caccatttgg ggattgaata cttcgtggag aaaagaatca caatggctaa
     5101 ccaaccaatt agacaagttg ccatttgtaa atgctaccaa accttcaact attgccttaa
     5161 atgcagaagt tgcccaattg attccaggac attcttctgt tatcagtgcg gctggtctgg
     5221 cttatattga cgattttgaa tcaacacaaa caaccataga cattcattat ccagctttct
     5281 ggtatctggc aagcacacct tataatccaa atggtgggct tttcccagaa gcagctttaa
     5341 gtaacaatgt tgaatatggt aaaaatagag ctttgttagc gtggtattat gttgatccta
     5401 ttttaaatgc aaacagcaga aatacacctc caaatttaag aaataatgtt gaatctcaat
     5461 ctaatcattt gacacgtaat gttctggaag aagaaatttt tccaaataaa gaacgactgg
     5521 caacacaaac ttcacgtttg actattttaa atttatctta ttatcctact gaaagaggtc
     5581 cttataatct ggatgcagat aatataaatc ctgatggaac cttaatgaat ccacaacaac
     5641 gctggggtgg tattatgcga aaactcgatg tgactgattt tgaaacagct aacattgaat
     5701 acattgaatt ctggatgatg gatcctttca ttatggataa agatggaaca cagagaggtg
     5761 gtgatttgta ttttaattta ggagatataa gtgaagatat tttaaaagat ggcaagaaat
     5821 tttttgaaca gggcttgcct cttgatgccg atattacaaa agtggatact acggtatggg
     5881 gaaaaattcc aaagacacaa tctactgtca aggcttttga taattctcct ggagcccgag
     5941 ccaaacaaga tgtcggttta gacggacttc ctacaaacga agaacaacaa tttcctactt
     6001 ataaaaatta tgtggagaaa attaatcaaa aaattgatgg tgaaacccgc caaaaatggc
     6061 aaagtgatcc tttttctcct ttaaatgatc ctgcggggga taactaccat ttttatagag
     6121 gttcagatta cgatgctcag gaaaaagata ttttatctcg ttacaaatat tataatggaa
     6181 cggaagggaa ttcgcccgaa gctgaaaata cacaggaaaa ctatagtact tctgctactt
     6241 ctttacctga tggagaggac ttaaatggtg ataatacgat gaatgaatat gaaaaatatt
     6301 atcaatatca tgttcaaata aaaagagaag caatggaagt tggtagaaat tatattactg
     6361 acaagattgt ttcaaatgtg aaattggaaa acggtaaagt tgagccagtt acctggtatc
     6421 aatttaaaat tcctattagg gaatatgacg aaaaaattgg taatatccgt aattttaaat
     6481 ctattcgttt cataagattg tttttgacca attttgaaca agaaacacat ttacgttttg
     6541 ctacactcga tttagtaaga ggtgaatggc gaagttatac taaatcactt tttccagcag
     6601 gctcaactcc aatttcagaa gggaaactcg atgttcattc tgtcaatata gaagaagatg
     6661 cagataaaac tcccgtcaat tacattttac ctccnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnaattat tacgaatatg aagtccctct tcgcgtgacg ccacatggca
     6841 aatatttgaa tgaaaaaaat gaagatcggg aaattgtttg gcctcttgaa aacatgtttg
     6901 attttccttt ttctacttta attgaagcaa aactgaaacg cgataaatat ctgcaaactt
     6961 ctggaggaaa cgccccaact ttaacaccct atgaagtgta tgatccagat aaccctaaaa
     7021 ataaaattaa aattgttgga aatccttcta tttcagatat tgaaaatatc atgatcggta
     7081 ttcgaaatgt aagtggcgaa attaagtctg gagaaatttg ggtcaatgag cttcgcatgt
     7141 caaaattcaa tgaatcaggt ggttgggctg caatgggaaa tttagcggta ggactttctg
     7201 atattggttc acttaatttt tcaggtcgta tagaaacagc aggttatgga agccttgaaa
     7261 gtaatgtgat gaatcgccga ttggaagact tgcgacagat gaatttttca actgccttag
     7321 aagtaggaag gtttttacct gaaaaagcaa agctgcaaat tcccctctat ttttcttata
     7381 cgaatgaaac tgtttcgcct aaatataatc cactgaatca agatgtcgaa ttaaaagatg
     7441 cactggaaaa tttaacttca aaaacagaac gagattcctt attaaatctt tcacaagaag
     7501 taaatacttc taaaagtttc aatatctcta atgcaagaat taacataaga agtaaaaaac
     7561 cccaatttta tgacccagct aattggcgat ttacttatgc ttatattgaa tccgataaat
     7621 atactcctga aattgagcag gatatgaata aaagtcaacg agctgccatt gactatagtt
     7681 ttaattttaa tccacaaccg tgggaaccat tcaaaaatat caaatcatta aataaaccag
     7741 ctttaaaaat cataaatgat ttcaacattt attatttgcc ttcttctatt aattttaata
     7801 ccaatttgaa tcgtcagtat tctcaaacta aattgcgagc acttgaagtt tcatctgttg
     7861 gtacttccga taaattagaa aatttatcat ttagcaagga ttttatgttt aatcgtcaat
     7921 ttgaaataaa atatgattta actcgtgcta tgaaattcag tcttcaagca gcaatgaatg
     7981 ccaatattga tgaaccttat tttacccctg aaataggaaa agaatattat gaagcctgga
     8041 gagatacagt atggtataac attcgtaagt tgggaactcc ttatgcttat cagcaagtgt
     8101 ttactgcttc gtggaatata cctatcaata aaattcctat aacaagttgg attgatgcca
     8161 acatttctta taattcaaat tataattgga acaggagtgc tcttctggag aaagatgggg
     8221 aaggtttagg aaacattgca accagcatag gttcatggca aacgaacgga cagtttaatt
     8281 ttgaagggtt atatagaaaa tctaattttt tgaaaaatgt attgaatcgt aacagtagct
     8341 ttgctacttc tcaaagacaa acttttaaac ctcgtgaata caaacagatt gttaatttgt
     8401 caaataatca aactttaacc gtacgtcata atttgggtag tgagtctgtt cgcttaaaag
     8461 caattgacaa aaatggaaat actattccgt tgcaatataa agtacgcagt aattctacgg
     8521 ttgatatttt cccaaaacag aatctggata gtgttcaact tatgcttact actatctcgc
     8581 ccaatgaacg ttctgtaagt ggtcaagtag ttgattttac attgggagct cttatgatgg
     8641 tacgccgtgt ttcttttact tatcgtgaat ctaacagtat ggttttaaca ggttttttgc
     8701 ccgaagctgg tttcctggga cagaaaaaat acaatggttt taacgctcca ggttatcctt
     8761 ttacgtttgg cattcacgat gataatatga tccaaaaagc tattgaaaaa ggttggttaa
     8821 atacgggcga ttcaattgta aatcctgcta caacagcttt tacttccgat tttgatttaa
     8881 aagcttcatt agaaccaatt cctggattta aaattgactt gaatgcaacc cgttatactg
     8941 cttccaatac ttcagtttta tttatgtacg aaggaatgcc aaccacttat acagggagtt
     9001 ttaacatcac acaaattgcc ttatccactg cttttaaaaa aataggttct gtggaaaata
     9061 attataattc ttctactttc aacatgtttt tacaaaatag gcaaaaaatt gctgatagac
     9121 ttaatagtaa atacatggga acaaattatc ctgctgctgg ctttattacg tcaaccaatc
     9181 tggcagggaa aaaatttgat ccttctgttg ggatttttag cttaaattcc cctgacgtac
     9241 ttatacccgc ttttttagct gcttatacag gaaaagatgc ttccaatgta aatacttctc
     9301 cttttatatc attattgaat atcttaccaa actggcgaat tagttatgat ggtctgtcaa
     9361 ggattggttg ggtgaaaaat cattttaaaa atataaatct gactcacggt tatacttgtc
     9421 gttatagtgt tggttcttac agttcttatt caacatgggt accgatgtca gaagatgaaa
     9481 cttttggtta tattcgtgat gttcagacca gtttgccaat tccgtcaagt gcttatgata
     9541 tatcaagtgt ttcaatcaac gaacagttta gtccattgct tggcatcaat accacaatga
     9601 ataatagtat gactacaaaa ctggaatacc gaaagcaacg aaatctttca ttaaatgtgt
     9661 cgagtgctca actgattgaa agtttgagtg atgaatatgt gattggattt ggatatgtac
     9721 ttaaagactt tgatgtgatt ttgaaattga aaacagataa acaatctcga ataaaaaacg
     9781 acttgaaatt gtcacttgat ctatcctatc gagacattaa aacgctgcta cgaaaaatcg
     9841 aagaaaacat aacacaagct tcaagtggta ataaactctt cacattgaaa tttttagccg
     9901 attatgtatt tagttcaaaa ttaaatattc agttatttta tgacagacaa tcaagtacac
     9961 c
//
LOCUS       DAOSAS010000061         9953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4461_length_9953_cov_14.010810, whole genome shotgun sequence.
ACCESSION   DAOSAS010000061 DAOSAS010000000
VERSION     DAOSAS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9953
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4461_length_9953_cov_14.010810"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /gene="sppA"
                     /locus_tag="PLR72_04150"
     CDS             <1..1677
                     /gene="sppA"
                     /locus_tag="PLR72_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOK36429.1"
                     /translation="IISGLASSKEKAPKLKPNSIYQLNLKGELVERSQEEPFSVALSQ
                     AFGKSDYQTLGLDDILKNIQKAKNNPDIVGIYLNCGPMVAGFASLKEIRDALVDFKKS
                     GKFIIAYADNYSQKIYYLASVADKVLLNPQGMLDWKGLSAQVVFFKNTLDKLGVEMQV
                     VKVGTFKSAVEPYVNTKMSDANRQQVTEYTQALWNTVLSEVSLSRKIPVDKLNAYADE
                     LITFQPAEKTLEYQLIDSLLYVEQVENLLKSLIKDTTLKELNYVKHSAMCKVPENVKF
                     ERNKVAILYGVGEIDMESRGGIQSEEFVKTINKIAKDNSIKAVVFRVNSPGGSAYASE
                     QIWHALTLLKEKKPLIVSMGDYAASGGYYISCMADSIVAQPNTLTGSIGIFGLIPNVQ
                     GLNNKIGITFDGVKTNKLSDAISVNRPFTPEEKDLMQNYVNRGYELFVKRCADGRRMK
                     IDQIKAIAEGRVWSGKEALKIGLVDKIGGIDEAVMMAAKKANLISYQIVEYPKKESFA
                     EKIMKSFSSHAETHFIKSKLGEQYQLFEEITNLNKVNGIHALLPFKLVVQ"
     gene            complement(1846..2292)
                     /gene="tsaE"
                     /locus_tag="PLR72_04155"
     CDS             complement(1846..2292)
                     /gene="tsaE"
                     /locus_tag="PLR72_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOK36430.1"
                     /translation="MKQPTTLKIPSLSSIHEVAKQFINQMGDDKIFAFYGKLGAGKTT
                     LIKGICKELGVKEMVNSPTFSMINEYETKEEEIIYHFDCYRINKIQEALDIGAEDYFY
                     SGKLCFIEWPENIEQILPDSTIKVYIEEKENQEREVKIIKPSKDKI"
     gene            complement(2370..4187)
                     /gene="uvrC"
                     /locus_tag="PLR72_04160"
     CDS             complement(2370..4187)
                     /gene="uvrC"
                     /locus_tag="PLR72_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOK36431.1"
                     /translation="MAQQDLLLQQLDMLPEKPGVYQFYNNKEEIIYVGKAKNLKKRVS
                     SYFNKTHESSRTNVLVKNIASLKYIVVDTEEDALLLENNLIKRYQPRYNVQLKDDKTY
                     PSICITNEGFPRVFKTRNIIQNGSQYFGPYSSVSALNSLLDTIRELYPIRTCKLPLTR
                     ENIKAGKFKVCLQYHIHKCNGPCEGKETVEEYQKNIQQIRQIIQGDANEISKMLWNEM
                     QQLAAEYKFEEAYAIKQKYDLIENYKAKSIIANTIVENTDVFAYDETENVAYITILRV
                     SKGAVIQGYTIEYQKKLEEDKADLLSTAIIELRQKLNSNSKEIVVPFEINYKPKDTQV
                     IIPQRGDRKKLLDLAIQNVQQYKLEKLKQAEKLNPEQRQIQLLKNLQEKLHLDKIPMV
                     IECFDNSNIQGSNAVAACVVYKKGKPSKNDYRKYDIKTVEGPDDYSSMKEVVRRRYTR
                     MLNEDKPLPDLIIADGGIGQMNVIREVLEDKLQIHIPIVGLVKDNKHNTRGLLYGFPP
                     KEVGLHPNDPLFRFMAAMQEEVHRFAITFHRNKRSKKQIASELDEIKGIGEKTKNELL
                     KHFKSVKRIKNATHDELSKIIGKSRASIIYNHFHTNLSL"
     gene            complement(4223..6097)
                     /gene="mnmG"
                     /locus_tag="PLR72_04165"
     CDS             complement(4223..6097)
                     /gene="mnmG"
                     /locus_tag="PLR72_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOK36432.1"
                     /translation="MDFKYDIVVVGSGHAGNEAACAAANMGSKTLLITMDMNKIGQMS
                     CNPAVGGIAKGQIVREIDALGGQMGIVTDRSAIQFRMLNRSKGPAMWSPRSQSDRHQF
                     IREWIKVISNTPNLYLWQDTVKELIIEDDTVTGVITALGVKISAKCVILSSGTFLNGL
                     IHVGKTQIRGGRISEPASFGLTEQLVSLGLKAGRMKTGTPARIDGRTIDFSQLKEQVG
                     DNDFHKFSFLEEVNRELKQMSCWITYTNPLTHEELRKGLADSPLYNGQIKSIGPRYCP
                     SIETKIVTFAEKQSHQLFLEPEGVDSQEYYLNGFSSSLPYPIQIKAIHTIAGLENAQL
                     YRPGYAIEYDFFDPTQLTHTLETKKIKNLFFAGQINGTTGYEEAAGQGLIAGINAHMK
                     CHGNAEFTLTRDESYIGVLIDDLVTKGVDEPYRMFTSRAEHRLLLRQDNADERLTEKG
                     YQIGLASQNRYYQYQRKKEEQAKLIQFIQHYSVKPQEVNAFLSARGLSELKHGCKLSD
                     IILRPQLNINDLGSVIPALKQKIDEIPSRREEIIESTEIILKYSGYIEREKMIAEKLQ
                     RLEHLIIRGRFDYDSILSLSTEARQKLMRVDPETIGQASRIPGISPNDINVLLVLMGR
                     "
     assembly_gap    6230..6329
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6330..6523)
                     /locus_tag="PLR72_04170"
     CDS             complement(<6330..6523)
                     /locus_tag="PLR72_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOK36433.1"
                     /translation="MSENLFIPINETKEEIYNNLLPQVAALIEGENDFIANLANISAA
                     LHETFHWLWVGFYLVKNNELV"
     gene            6738..9566
                     /locus_tag="PLR72_04175"
     CDS             6738..9566
                     /locus_tag="PLR72_04175"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOK36434.1"
                     /translation="MINKDFRVKRFILLTALTLFSLSLFSQQQFQPLPIDPKVRYGKL
                     ENGLTYYIRSNQFPKQRADFYIVQKVGAILEEDYQNGLAHFLEHMAFNGTVHFPGKKI
                     IDYFESIGVKFGQNINAYTSLDETVYHLSNVPTIRESIIDSALLVLHDWSHFILLEDD
                     EIEAERGVILEEWRQGQNAYRRMWAESNKKKFPGSQYAKRDIIGDTAVIKNFKPDELR
                     EYYNKWYRPDLQAIIIVGDVDVDKVEAKIKELFTDIKKNENAPERPIYPIFDNEEPII
                     SIVTDPEAPQTQIELYYKHNKLPAEIKLSMNGYVIELINSLISNIMQDRFDEITMKPD
                     APFVAGYANYGELVKSKDAFQLLVIPKEGQELEGLEALLLEAEKIKRFGFVNSELERA
                     KANLIKQVEKRYNERENLKNSEFVKEYIGHFLNNEPIPGIEWEYQTLQMILPQVNVDM
                     VNQLAKSYVTDTNMIVSIMAPEKETVKIPTEEQIRQLIENTKTVELTPKVDEQINKPL
                     IGQVPKAGKIKKVTKNSTLETTEWLLSNGVKVILKPTKFKKDEILLNAYSIGGLAKII
                     NNEDLISGAFASEIVSNNGLAEFSQIDLKKLLTGKIVSVRPYISSYEEGFSGNSSVSD
                     FETMLQLIYLHFTALRRDDDAFTALMNTYKAILSNSEKNPDKAFSDSITVMLTNHHPR
                     TILLNLSTIEKVNQDKALSIFAERFANPADFTFIFTGNIDPQNESLKKFICTYLGGLR
                     TTNMKETFTDDQIRKPEGRVNNHFTKEMKVKKASNFIAYHGQMPFNLQNRTAMSAIGN
                     ILNIRYLESVREKEGGSYGVGVRGSIDNKPIEDAILMIQFDTDPEKQPKMISIIYDEI
                     NNIIANGPRKDDVQKVKETMLKKYAEDVENNGWWQNAIYLYYQDHLNLVTDYKSSVEE
                     VTPLFIQTTLKKLVDQNNVIEVIMMPKE"
     gene            9692..>9953
                     /locus_tag="PLR72_04180"
     CDS             9692..>9953
                     /locus_tag="PLR72_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36435.1"
                     /translation="MMRKIIIYKKWFLFFGFWIMFCQGHSQETNYFFYVQLSDKKNSP
                     FSLEEPLEYLSERAIERRKFFSIPLDSTDLPINPSYLNLIQQT"
BASE COUNT         3318 a         1573 c         1599 g         3363 t
ORIGIN      
        1 attattagtg gattagcaag ctcaaaggaa aaagcaccca aattaaaacc aaattctatc
       61 tatcaattga atttaaaagg tgaattggtc gaacgatcac aagaagaacc tttttcagtt
      121 gctttatcac aagcttttgg aaaaagtgat taccaaactt tgggattaga tgatatttta
      181 aaaaatatcc aaaaagcaaa aaataatcct gatattgttg gaatatattt gaattgtgga
      241 ccaatggtag ctggctttgc atctctaaaa gaaattcggg atgctttagt tgattttaaa
      301 aaatccggaa aatttattat tgcctatgct gataattatt ctcaaaaaat atattattta
      361 gcgtcggtag ccgacaaagt tttattaaat ccacaaggca tgcttgattg gaaaggattg
      421 tccgcacaag tagttttctt taaaaacacc ttagataaat taggtgttga aatgcaggta
      481 gttaaagtcg gaacttttaa atcggctgtt gaaccttatg taaataccaa aatgagcgat
      541 gccaatcgcc aacaagttac cgagtacaca caagctcttt ggaatacagt tttatcagaa
      601 gtgtcattat cccgtaaaat cccagtagat aaattaaatg cttatgcaga tgaattgata
      661 acttttcagc ccgccgaaaa aactttagaa tatcaactaa ttgactcatt attgtatgtt
      721 gagcaagtag aaaacctgct gaaatctttg ataaaggata ccactttaaa agaactgaat
      781 tatgtgaagc actctgctat gtgtaaagtt ccggagaatg taaaatttga aaggaacaag
      841 gtggctatcc tctatggagt aggagaaatt gatatggaga gtagaggagg cattcaatca
      901 gaagaatttg taaaaactat taataaaata gcgaaagaca attccattaa agccgttgtt
      961 tttagagtaa attccccagg aggaagtgcc tatgcttctg aacaaatatg gcatgctctt
     1021 actttattaa aagaaaaaaa acctttgatt gtatctatgg gcgattatgc cgcttcagga
     1081 ggatactata tttcatgtat ggctgatagc attgtagctc aacctaatac gttaacaggt
     1141 tcaattggaa tttttggctt gatacctaat gttcaaggat tgaacaataa aattggaata
     1201 actttcgatg gagtcaaaac caataagttg agtgatgcta tttctgtaaa tcgcccattt
     1261 actcctgaag aaaaagattt aatgcaaaat tatgtcaacc gaggttatga gttatttgtc
     1321 aaacgttgtg ctgatggtcg aagaatgaaa attgatcaaa ttaaagcaat tgcagagggt
     1381 agagtttggt caggtaaaga agcattaaaa attgggttgg tagataaaat tggcggtatt
     1441 gacgaagctg ttatgatggc tgctaagaaa gcaaatctaa tttcttatca aatagttgaa
     1501 tatcctaaaa aagaaagttt tgccgaaaaa ataatgaaaa gtttttcttc tcacgctgaa
     1561 acacatttta taaaatccaa acttggcgaa caatatcaat tattcgaaga aataaccaat
     1621 ttgaataaag tcaatggaat tcacgctctt ttacctttta aattagttgt tcagtagttt
     1681 tttaatacgt aaatgctatc aagaagaggc aaatacagtt ctggtttgcc tcttcttata
     1741 tgtgttagac gtgtagcacc ataatgtttg acatatttct aattaataag attcaaaaag
     1801 agagacttaa ttttaaataa taagttctca atcaattaat taggctcaaa ttttatcttt
     1861 tgagggttta attattttca cttctctttc ctgattttct ttttcttcaa tataaacttt
     1921 tatagtagaa tctggtaaaa tctgctcaat attttctggc cattcaatga aacaaagttt
     1981 tccactgtaa aaataatcct ctgctccaat gtctaaagct tcctgtattt tattgatacg
     2041 atagcagtca aaatgataaa ttatttcttc ttcctttgtt tcgtattcgt ttatcataga
     2101 aaatgtagga ctgttgacca tttcttttac tcccaattct ttacaaatac ctttaatcaa
     2161 agttgttttt ccagctccta attttccgta gaaagcaaat attttatcat cgcccatttg
     2221 gttgataaat tgtttggcta cctcatgaat agaagaaagc gaaggaattt ttaaagtagt
     2281 tggctgtttc attattttga tttttgttaa tctttacgag atgctcgtat ttacgtataa
     2341 cttatcaatt tttttgaaat atcttattct cacaagctca aattggtatg aaaatgatta
     2401 taaatgattg atgctctgct ttttcctata attttagaga gttcatcatg cgttgcattt
     2461 ttaatgcgtt ttacgctttt gaaatgtttt aaaagttcat tttttgtttt ttctccaatt
     2521 cctttaattt cgtcaagttc tgaagcaatt tgttttttac ttcgtttgtt gcgatgaaaa
     2581 gttatggcaa aacgatgtac ttcttcttgc attgctgcca taaatctgaa caatggatca
     2641 tttggatgca atccaacttc ttttggtgga aaaccataca gcaagcccct cgtgttatgt
     2701 ttattgtcct ttaccagtcc aacaatcgga atatgaattt gtagtttatc ttccagtact
     2761 tctctgataa cattcatttg tccaataccg ccatcagcaa tgattaaatc aggtaaaggc
     2821 ttatcttcat ttaacattct ggtatatctt cgtcttacaa cttctttcat ggaagaataa
     2881 tcgtcaggac cttcaacggt tttaatgtca tatttcctgt aatcattctt cgaaggtttg
     2941 ccttttttat aaaccacaca tgctgctaca gcatttgacc cttgaatatt tgaattgtca
     3001 aagcattcaa tcaccattgg aattttatcc aaatgcaatt tttcctgcaa atttttcaac
     3061 agttggattt gtctttgttc tggattcaat ttttcagctt gcttgagttt ttccagcttg
     3121 tattgttgaa cattttgtat agcaagatca agtaattttt ttcggtctcc ccgctgagga
     3181 ataattactt gtgtatcttt cggtttgtaa ttaatttcaa aaggtacaac aatttctttt
     3241 gaattgctat ttaatttttg tcgaagttca atgatggctg tcgagagcaa atcggcctta
     3301 tcttcttcca gttttttctg atattctatt gtatatcctt gaatgacagc accctttgaa
     3361 actcttaaaa tagtaatgta agcaacattt tcagtttcat cataagcaaa aacatctgta
     3421 ttttcaacta tcgtgttggc aataattgat tttgctttat agttttcaat caagtcatat
     3481 ttctgtttta ttgcataagc ttcttcaaat ttatattcag cagcaagttg ttgcatttcg
     3541 ttccagagca ttttactgat ttcgttcgca tcgccttgaa taatctgacg aatttgctgg
     3601 atattttttt gatattcttc aacggtttcc ttcccttcgc acggaccgtt gcatttgtga
     3661 atgtgatatt gcaggcaaac tttaaattta cctgctttta tattttctct ggtcaatgga
     3721 agtttgcaag ttcggatggg ataaagttca cgaatggtat cgagtaatga atttaaagca
     3781 cttaccgaac tataagggcc aaaatattga gaaccatttt gaataatatt tctggttttg
     3841 aaaactcttg gaaatccttc gttggtaata cagatgcttg gataggtttt atcatctttt
     3901 aattgaacat tgtatcgtgg ttgatatcgt ttaattaaat tattttcgag cagcaacgca
     3961 tcttcttctg tatctactac aatatatttt aaagaagcaa tatttttgac taaaacattc
     4021 gttcgtgacg attcatgcgt tttattaaag taagatgaaa ctcttttctt aagatttttt
     4081 gctttgccaa cataaattat ttcctcttta ttattataaa attgatacac tcctggcttc
     4141 tcaggaagca tatccagttg ttgtaacaat aaatcttgtt gagccatttt gcaaaataat
     4201 gtttcacgtg gaacatttaa aattatcttc ccattaaaac caataacacg ttaatgtcat
     4261 ttggagaaat tcctggaatt cggcttgctt gcccaatagt ttcaggatcg actcgcatca
     4321 atttctgccg agcttcggtt gataaagata aaatagaatc ataatcaaaa cgtccacgaa
     4381 tgattaaatg ttctaatcgt tgaagttttt ctgcaatcat tttttcacgc tcaatatagc
     4441 cactgtattt caaaataatt tcagtagatt cgataatttc ctctcgtcga gagggaatct
     4501 catcaatttt ttgttttaaa gcaggaataa cagagcccaa atcattgatg tttagttgag
     4561 gccttaaaat tatatcagat aatttacaac catgctttaa ttctgataaa cctcttgcag
     4621 aaagaaaagc attcacttct tgaggcttta ctgaataatg ttgaataaat tgtataagtt
     4681 ttgcttgttc ttcttttttt ctttggtact gataatagcg attttgtgaa gccaatccta
     4741 tttgatatcc tttttcagta agacgttcat cagcgttatc ctgacgaagg agtaaacgat
     4801 gttcagcacg ggaagtaaac attcggtaag gttcatcaac tcctttggta accaaatcat
     4861 caattaaaac tccgatataa gattcatcac gagtcaaagt aaattcagca ttgccatgac
     4921 acttcatgtg agcattgata cctgcaatca aaccttgtcc tgcagcttct tcatatcccg
     4981 ttgttccatt aatttgtcca gcaaaaaaca gattttttat tttctttgtt tccagtgtat
     5041 gtgttaattg ggtaggatca aaaaaatcat attcaatggc ataccccggc ctgtaaagtt
     5101 gagcattttc aagacccgca atggtatgaa tagctttaat ttgaatggga tacggaagtg
     5161 aagaggaaaa tccattgaga taatattcct gtgaatccac cccttccggt tcaagaaaaa
     5221 gttgatgact ttgcttttca gcaaaagtta caatttttgt ttcaatactg ggacaataac
     5281 gtggaccaat actttttatt tgtccattgt ataaaggcga atccgccagt cctttcctta
     5341 attcctcgtg ggtgagagga ttagtataag taatccagca actcatttgt tttaattcac
     5401 gattcacctc ttctaaaaag gaaaatttat gaaaatcatt gtcgccgact tgttctttaa
     5461 gttgagaaaa atcaatagtt cgaccatcaa ttctggcagg tgttcctgtt ttcattcttc
     5521 cagcttttaa acccaatgaa acaagttgtt cagtcaaacc aaatgaagca ggttctgaaa
     5581 ttcgtcctcc tcttatttgt gtttttccaa catgaatcaa tccgtttaaa aaagttccag
     5641 aagaaagaat tacacattta gcagatattt tcactcccaa agcagtaata acgcctgtta
     5701 ccgtgtcatc ttcaattatt aattccttta ccgtgtcttg ccaaagatat aaattaggtg
     5761 tattactaat gacttttatc cattcacgaa taaattgatg acgatcgctt tgtgaacgtg
     5821 gactccacat ggcaggacct ttggaacgat ttaacatccg aaattgaatt gccgaacgat
     5881 cggtaacaat tcccatttga ccgcccaatg catctatttc gcgtacaatc tgtcctttag
     5941 caataccgcc tactgcagga ttacaactca tctgaccgat tttgttcata tccattgtaa
     6001 tcagtaaagt ctttgaaccc atattggctg cagcacaggc agcttcattg cctgcgtgtc
     6061 cactcccaac aactactata tcgtatttaa aatccattta tctcaaaaaa attaaaaaat
     6121 attgtaacaa ccgataaaat ttttagtgaa aaatccgttt cttgttgata attttttaaa
     6181 catgaaaaaa attagataat ttattggtaa tcaattgaca aatttttccn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ccaattcatt attttttacc agataaaaac
     6361 ctacccagag ccaatgaaaa gtttcatgca aagcagcaga aatatttgct aaatttgcaa
     6421 taaaatcatt ttccccttct atcaaagcag ccacttgagg taaaagatta ttataaatct
     6481 cctcttttgt ttcatttatc ggaataaata aattttcact cataaaaaat tatttattta
     6541 catttctact acaaaattac attaaaaaat cttttctgag agttatttca attcttagtt
     6601 tcatttgaga acatagtaat tacaagattt aacattttta agaaaaagca aattgattta
     6661 ttgtactttc taaaatttat ctgttacttt gtgaattaaa attgtttttt aaagttcatt
     6721 tttaaaaatt ttttattatg ataaataaag attttagagt taaaagattt attttattaa
     6781 ctgcactgac gcttttttct ctttcgttat tttcacaaca acaatttcag cctcttccta
     6841 ttgatccgaa agtaaggtat ggcaaattag aaaatggact cacgtattac attcgatcca
     6901 atcaatttcc caaacaacgt gctgattttt atatcgttca aaaagtgggc gctattttag
     6961 aagaagatta tcaaaatggt cttgcacatt ttcttgaaca tatggcattt aatggaactg
     7021 ttcatttccc cggaaaaaaa attattgatt attttgaatc aattggtgtt aaatttgggc
     7081 aaaatatcaa tgcctataca tcccttgatg agacagtgta tcatctttcg aatgtaccaa
     7141 ctattcgtga aagtattatc gacagtgctt tgttggtttt acatgactgg tcacatttta
     7201 tattattaga agatgatgaa attgaagcag aaagaggtgt aattttagaa gaatggcgac
     7261 aaggacaaaa tgcttatcgc cgcatgtggg ctgaatcaaa taagaaaaaa tttcctggtt
     7321 cacaatatgc taaacgggat ataattggag atacagcagt gattaaaaat tttaaaccag
     7381 atgagcttcg tgaatattac aataaatggt atagaccaga tttacaggca ataataattg
     7441 taggtgacgt tgatgtagat aaagttgaag caaaaattaa agaattattt acagacataa
     7501 agaaaaatga aaatgctcca gaacgtccaa tttatccaat ttttgacaat gaagaaccaa
     7561 ttatctcgat tgtaacagat ccagaagctc ctcaaactca aatagaattg tattacaagc
     7621 ataataaact accagcagag attaaattgt caatgaatgg ttatgtgatt gagcttatta
     7681 attcgttgat ctccaatatt atgcaagatc gatttgatga aattacaatg aaacccgatg
     7741 ctcctttcgt ggcaggttat gctaattatg gagaattggt taaatcgaaa gatgctttcc
     7801 agttattagt tattccaaaa gaagggcaag aattagaagg attagaggct ttgctcctcg
     7861 aagccgaaaa aatcaagcgt tttggtttcg taaattcaga gttagaaagg gcaaaagcaa
     7921 atttaataaa gcaagttgaa aaacgatata atgaaagaga aaatctaaaa aatagcgagt
     7981 ttgtcaagga atatataggt cattttttaa ataacgaacc tattcccggt atcgaatggg
     8041 aatatcaaac attgcagatg atactaccac aagtaaatgt agacatggta aatcaattag
     8101 ccaaatcata tgttacagat acaaatatga ttgtttctat tatggctcct gaaaaagaaa
     8161 ctgttaaaat ccctactgag gaacaaatcc gacaattgat cgaaaatacc aaaactgttg
     8221 aattaacacc aaaagttgat gagcaaatta ataaaccatt aattggacaa gtgcctaaag
     8281 ctggaaaaat aaaaaaagta actaaaaatt caacactgga aacaaccgaa tggttattaa
     8341 gcaatggtgt gaaagtgatt ttgaaaccga ctaaattcaa aaaggatgaa attttgctga
     8401 atgcatatag catcggaggt ttggcaaaaa ttataaataa tgaagattta atttctggtg
     8461 cgtttgcctc tgaaattgtt tcaaataatg gtttagcaga attttcacaa atagatttaa
     8521 aaaaattact aacagggaaa attgtcagtg ttaggcctta tatatcttct tatgaggaag
     8581 gtttctcagg gaattcttcc gtttctgatt ttgaaaccat gttacagtta atttatttgc
     8641 attttacagc tttacggaga gatgatgatg cttttacagc tttaatgaat acttataaag
     8701 ctattttgtc taatagtgaa aaaaatcccg ataaagcctt ttctgatagt attactgtta
     8761 tgctcacgaa tcatcatcca cgtactattt tgttgaattt gagcacaata gaaaaagtaa
     8821 atcaagataa agcattgtcc atttttgctg aacgatttgc caatcctgct gattttactt
     8881 tcatctttac aggaaatatt gatccacaaa atgaatcatt gaaaaaattt atttgtactt
     8941 atttgggagg tttacgtacc accaatatga aagaaacatt tacagatgat cagataagaa
     9001 aacctgaagg gagagtaaac aatcatttca caaaagaaat gaaagtaaaa aaagcttcaa
     9061 attttattgc ttatcatgga caaatgcctt ttaatttaca aaacaggacg gctatgagtg
     9121 ccataggaaa tattttaaat attcgttatc tggaaagtgt tcgtgaaaaa gaaggtggtt
     9181 cttatggtgt tggagtgcgt ggaagtattg ataataaacc gattgaagac gcaatactta
     9241 tgatccaatt tgataccgat ccagaaaaac aacctaaaat gatcagcatc atttacgatg
     9301 aaatcaataa tatcatcgcc aatggacccc gcaaagatga tgtgcaaaaa gtaaaagaaa
     9361 caatgttgaa aaaatacgct gaagatgtag aaaataatgg ctggtggcaa aatgctatct
     9421 atctttacta tcaagatcat ttgaatttag taaccgatta taagtcttcg gtagaagaag
     9481 ttacaccatt attcattcaa actacgctga aaaaactagt cgatcaaaac aatgtgatag
     9541 aagtaataat gatgcctaaa gaataataaa ataccaaatt ttcaaagagg aagccaaaaa
     9601 tcttcctctt tgttcaaaat aaacattatt aaccctagct aatttattgg aattgaatac
     9661 aaaaatattt ttaaaatggt aatattcagg gatgatgaga aaaattatca tttataaaaa
     9721 gtggttttta ttttttggtt tttggataat gttttgccaa ggtcattctc aagaaaccaa
     9781 ttattttttt tatgttcaat tgtcagataa aaaaaatagt ccgttttcat tagaagaacc
     9841 attggaatat ctttcagagc gagccataga acgaagaaag tttttttcta ttcctttaga
     9901 ttcaactgat ttaccaatta atccatctta tttaaattta attcaacaaa cag
//
LOCUS       DAOSAS010000062         9831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4525_length_9831_cov_14.802475, whole genome shotgun sequence.
ACCESSION   DAOSAS010000062 DAOSAS010000000
VERSION     DAOSAS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9831
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4525_length_9831_cov_14.802475"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..872)
                     /locus_tag="PLR72_04185"
     CDS             complement(231..872)
                     /locus_tag="PLR72_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36436.1"
                     /translation="MIIPSVFLAWNVVKESRFNTNAIRYVQAMQKLDLFTETEIINVN
                     REYSSKGGKIYLSLIGKPLNERQIQFLQNQLVEYGLKGVQLSIKQTTGTLDIGAQTAF
                     ISNLLDKKDAELHKKDSLIQALNEELLKIKNLGIDEIQIAREVKVQFPEIEKISFSHS
                     IFTDVHTLAQDTIATVYLIWKENPKNNELIKKLEEWLKVRLNWEKMKIIEINE"
     gene            complement(817..1614)
                     /locus_tag="PLR72_04190"
     CDS             complement(817..1614)
                     /locus_tag="PLR72_04190"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOK36437.1"
                     /translation="MENKEKKNLQKYWQKIYQKIRNITNLHEDTNIETTLESIKKDVE
                     FKGANVWILFFAVIVCSVGLNVNSTAVIIGAMLISPLMGPINGVGLAVGINDAELLRN
                     ALKNLLIMILVGLLASTLYFLITPLSDAQSELLARTRPTIFDVLIAFFGGLAGIVAAS
                     RKEGKITVIAGVAIATALMPPLCTAGFGLATGQMKYFGGAFYLLFLNSFFIALATLLM
                     VRYLHFPVKHFVDSTKESSPTFYYYIFYFSDYTKRFSCMECSQRKSI"
     gene            complement(1754..3877)
                     /locus_tag="PLR72_04195"
     CDS             complement(1754..3877)
                     /locus_tag="PLR72_04195"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOK36438.1"
                     /translation="MKQFIIFILLFCSLIVAKADEGMWMLPLIEKLNINKMHEMGCTL
                     SAKDIYNNDSISLKDAVIIFGNGCTGVMVSVDGLVFTNHHCGFNAIQQHSTIEHNYLK
                     NGFTAQKLEDEIPTPGLTVTFLVRIEDVTNNILSQIPDSLTGINREDKISIISDSLSH
                     AAIKDSSYTAKVLPFFNENQFYLFVYEKFEDVRLAFAPPSSIGKFGGDTDNWMWPRHT
                     GDFSVFRVYCSPDGKPATYSIDNVPYSPKRFVPISNKGYEENDFTMILGNPGSTNRYL
                     SSFGIDYRMKVLNQARIDVRGIKQDIWRSFMNSNEAIQIAYANKHASSSNYWKNSIGM
                     NQAIVKLKVMERKKAEEAQFTEWLKTSPERQKKYNDVLTSLEEGYAKMYPYGKALIYM
                     YESLINGVELPRIAENIQQWHNFNFSNDEILKKAEKLYKDYYPEVDQKVFIAMLKVYK
                     KSVDADALPEIYEVIQKKFKGEEEKYVQYLFDKSSFTTYEKLKKALLNNKIDFKNDPA
                     LVFSSVVKQTYNNINLKLSPINDQIKEVEKIYMAGLMEKAQEEGKQLYPDANFTMRMT
                     YGKVAGYVPADAVTYRYYTTTKGILEKENPNNEEFIVPSSLKKAIENKNFGNYRDKNT
                     GEMQVNFITNNDITGGNSGSPIFNAKGELIGLAFDGNWESLSGDIIFEPDLQRTIGVD
                     IRYMLFVMDKIGNAERLLKELNICN"
     gene            complement(4015..5031)
                     /locus_tag="PLR72_04200"
     CDS             complement(4015..5031)
                     /locus_tag="PLR72_04200"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOK36439.1"
                     /translation="MEIDWNNLGFGYIKTDYNLRCTYKDGKWGDFEVHDDEYLTLHMA
                     STCLHYGQEVFEGLKAFRGKDGKIRIFRMDENAKRMQSSCAGIVMAQLPVEKFEEAVL
                     KVVKLNERFVPPYESGASLYIRPFMIGTSAQVGVKPAREYQFIIFVTPVGAYFKGGFK
                     ATPFVIFREYDRAAPHGTGTYKVGGNYAASLVPDEKAHAMGYSAVFYLDPKEKKYIDE
                     CGAANFFGIKNNTYITPKSTSILPSVTNKSLMVLARDMGMKVEQRPVPLEELSTFEEA
                     GACGTAAVISPIQRIDDIEENKSYIFSPDGKPGVICEKLYNKIRAIQYGDEPDIYGWT
                     TIVE"
     gene            complement(5095..5706)
                     /locus_tag="PLR72_04205"
     CDS             complement(5095..5706)
                     /locus_tag="PLR72_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOK36440.1"
                     /translation="MPAILSLAYLAPVEYYKVLANSSEIFIEKEENYIKQTYRNRCKI
                     LTANGVMDLIIPVEKSEGNKPSIKDVKISVHSDWQKQHWRAIVSAYSSSPFFEYYEDD
                     LYPFFKKKWLFLWDFNLEIQNKIIELLDLETQINFTEKFEPFPADKRIDLREEFHPKR
                     QSKIEYSPYYQVFRGKFGFVKGLSVIDLLFNMGNESQLILSEK"
     gene            complement(5789..7189)
                     /locus_tag="PLR72_04210"
     CDS             complement(5789..7189)
                     /locus_tag="PLR72_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOK36441.1"
                     /translation="MVSQKFRTRFHQPLKQWVKAGIWSLIYILFIAWVNNFWWLLLLP
                     FIFDIYITKFIPWTWWKKVKNKSLRSFLSWVDAIIFALVAVYFINLFLFQNYQIPSSS
                     LEKTLLVGDFLFVSKVNYGPRVPFTPLSFPLVQNTFPIINTKSYLEKPQWDYKRLKGL
                     DTIARNDIVVFNFPTGDTVALKQQNPDYYQLCYYYGREVVHSRKDIFGEIVYRPVDRR
                     ENYVKRCVAVAGDWVEIRNNQVYVNGKKEEKFPGIQFNYLVQTDGRRLSDELLSKLHI
                     SKDDRLLLNTMQNPGEIISLGLDPELPIYHFPLTEEAYNKLRNVSGVTKIIVEPDQYG
                     GEVFPLGGGKNWTRDNYGPIYIPRKGAKLVLNAYNYPIYERVIRVYEKNKLEMKEGKF
                     YINDKPATTYQFKMNYYWMMGDNRHNSADSRYWGFVPEDHIVGQPVFVWLSLDKDKSW
                     FNGKVRWKRFFKNATR"
     gene            complement(7331..8047)
                     /gene="dapB"
                     /locus_tag="PLR72_04215"
     CDS             complement(7331..8047)
                     /gene="dapB"
                     /locus_tag="PLR72_04215"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOK36442.1"
                     /translation="MKLALIGYGKMGKTIEKIARERGHKIVSIIDVNNLEDFDSENFK
                     QADVAIEFTNFKSALDNYKKAFEHNIPVVSGTTGINDELPLLKKEVEENGYTFLWSSN
                     FSLGVNILLAVNKYLSNIMNQFSEYDVNITEIHHTQKLDVPSGTAITLAEGIIEKLNR
                     KKKWVKDVQKNSDEIAIQSIRTDDVPGIHTVRYESQIDTIEITHNAKNRDGFAVGAVL
                     AAEFLAGKKGWFTMADFLKF"
     gene            complement(8148..9203)
                     /gene="aroB"
                     /locus_tag="PLR72_04220"
     CDS             complement(8148..9203)
                     /gene="aroB"
                     /locus_tag="PLR72_04220"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOK36443.1"
                     /translation="MLQGSTIICNDFVSELSEVIGNQESESIFILVDENTQRLCLPTI
                     MKINKLQNSYVVSIPAGDEHKNIETAIQIWQYLSEHGATRQSLMINVGGGMVTDIGGF
                     TAATFKRGIRYLNIPTTLLGAVDAATGGKTGINFLGLKNEIGVFAPAEKVLINVDFFK
                     TLDDKNFRSGFAEMVKHALLDSRKEWNDILTIDLDNIDFDYLKQKVEKSVHIKEAIVE
                     KDPKEQNLRKALNLGHTFGHAFESFSLLSNKPVLHGYAVMWGLLCELYLSVIRFNFPQ
                     EDLLKLKYLIKEYYGIFFIPCENYEKIYELMTHDKKNRSKEINFTLLSDIGQIQLNQT
                     ASKEEIFECLDFLSNGL"
     gene            9387..9680
                     /locus_tag="PLR72_04225"
     CDS             9387..9680
                     /locus_tag="PLR72_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOK36444.1"
                     /translation="MLKNTKQIEEAAYLLKAIANCTRLGIILLLSEKDELNVSQLQEE
                     LNCEQSLLSHHLTDMRAKGILNCRRESKNCYYSVKNKKIVQVIECILSCQNQN"
BASE COUNT         3333 a         1773 c         1356 g         3369 t
ORIGIN      
        1 ttatttctta gaacattcca tctttggttg taattaatag aattttacta tttatttcta
       61 cttcttatca accaatagtt tagctttaaa atttgtatct aagtgttttt taaatattca
      121 aaaccttcct caattagaag caaatataaa actttatctc acttgttaaa tataaatact
      181 cctctcctga aaatgaaatc aacgaaaata attgttattt ttcaaaaaag ttattcattt
      241 atttctatta tcttcatttt ttcccaattg agccgaactt ttaaccattc ttctaatttt
      301 ttgattagtt cattgttttt aggattctct ttccatataa gataaacagt ggcgatagta
      361 tcttgagcaa gggtgtgcac atcagtaaaa attgaatgag aaaaagaaat tttctctatt
      421 tcaggaaatt gcacttttac ttcacgtgct atttgtattt catcaattcc taaatttttt
      481 atttttaaca attcttcatt gagagcctga atcaaagaat cttttttatg aagttcagca
      541 tcttttttat ctaataaatt tgaaataaaa gcggtttgag ctccgatatc caaagtacca
      601 gttgtttgtt tgatactcaa ttgaactcct tttaacccat attctactaa ttgattttga
      661 agaaattgta tttgcctttc attcaacggt ttaccaatca aagaaagata aattttccca
      721 ccctttgaag aatattctct atttacatta ataatttcag tttcagtaaa taaatcaagc
      781 ttttgcatag cttgaacata tcgaatagca ttggtattaa atcgactttc tttgactaca
      841 ttccatgcaa gaaaaacgct tggtataatc actaaaatag aaaatatagt aatagaacgt
      901 cggactactt tcttttgttg aatccacaaa atgtttcact ggaaaatgca agtaacgcac
      961 cattaaaagg gtagctaaag caataaaaaa agaattcagg aaaagcaaat aaaaagctcc
     1021 cccgaaatac ttcatttgtc ctgttgctaa tccaaaacct gctgtacaca aaggtggcat
     1081 cagtgcagta gcaatggcaa ctccagctat tacagtgatt ttaccttctt tgcgtgaagc
     1141 tgctacaatt ccagccaaac caccaaaaaa agctatcagt acatcaaaaa tcgttggacg
     1201 tgttcttgcc aacaattcgg attgagcgtc acttaatgga gtaataagaa aataaagagt
     1261 agaagctaaa agccctacta aaatcataat caataaattt tttagtgcat ttcgtaataa
     1321 ttctgcatca ttaataccaa ctgctaatcc aactccatta ataggtccca tcaaaggaga
     1381 aattaacata gcaccaataa ttactgccgt agaatttaca tttaatccta ctgaacaaac
     1441 aatcacagca aaaaacaaaa tccaaacatt tgcacctttg aactccacat ctttttttat
     1501 agattcaaga gtggtttcga tgttggtatc ttcatgaaga ttggtaatat ttcttatctt
     1561 ctgataaatt ttttgccaat atttctgtaa atttttcttt tctttatttt ccataacttt
     1621 aagatttaag catataaacc aagttacatt tctaaacaaa ttgacacttc acaatttgct
     1681 tccaaaaaaa ttaaagaaca attttgtttt aaaacactaa aattcaataa acagtgaatc
     1741 taaaaagttt ctctcaatta caaatattta attctttcaa taatcgctca gcattaccta
     1801 ttttgtccat tacaaaaagc atataacgga tatctacacc aatagttcgc tgcaaatcag
     1861 gttcaaaaat aatatcacca ctcaacgatt cccaatttcc gtcaaaagcc aaacctatta
     1921 attcgccctt tgcattaaat ataggacttc ctgaatttcc accagtaata tcattgttag
     1981 taataaaatt tacctgcatt tcacctgtat ttttatctct ataatttcca aaatttttgt
     2041 tctcaatcgc tttctttaaa gaagaaggaa cgataaattc ttcattgttg ggattctctt
     2101 tttctaaaat acctttagta gttgtataat aacgataggt cactgcatca gccggaacat
     2161 aaccagctac ttttccataa gtcattctca tcgtaaaatt agcatcggga taaagttgtt
     2221 taccttcttc ctgagctttt tccattaatc cagccatata aattttttcg acttccttta
     2281 tttgatcatt gataggagat agttttaaat taatgttatt atatgtttgt ttaacaacag
     2341 aagaaaaaac taaagcagga tcatttttaa aatctatttt attatttaac aaagccttct
     2401 tcaatttctc ataagttgta aaactggatt tatcaaaaag atattgcacg tatttttctt
     2461 cctctccttt aaattttttc tggattactt cataaatttc aggaagggca tcagcatcaa
     2521 ctgatttttt ataaactttc aacatggcaa taaaaacttt ttgatcaact tctggataat
     2581 aatccttgta aagcttttcg gcttttttta atatttcatc attggaaaaa ttgaaattat
     2641 gccattgttg tatattttca gctattctcg gtaattcaac tccattaatt aacgattcat
     2701 acatataaat caaagcttta ccataaggat acatttttgc atatccttct tccagtgaag
     2761 tcaagacatc attatatttt ttctgtcgtt caggtgaagt ttttagccat tcagtaaatt
     2821 gagcttcttc tgcttttttc ctttccatga ctttcaattt tacaatggct tgattcattc
     2881 caatactgtt tttccaataa ttagaactgg aagcatgctt attggcataa gcaatttgaa
     2941 tagcttcatt gctgttcata aagctacgcc aaatatcttg tttgattcca cgtacatcaa
     3001 tacgagcttg attaagcact ttcatccgat agtcaatccc aaaagaagac aaataacggt
     3061 tggtagagcc aggattgccc aaaatcatgg taaaatcatt ttcttcatat cctttgttgg
     3121 aaatagggac aaaacgtttg ggtgaataag gaacattgtc tatagaataa gtagcaggtt
     3181 tgccgtcagg tgaacaataa accctgaaaa cagaaaaatc gcctgtgtgg cgaggccaca
     3241 tccaattatc ggtatcgcca ccgaattttc ctattgaact tggaggtgca aaggctaaac
     3301 gaacatcttc aaacttctca taaacaaaaa gataaaactg attctcatta aagaaaggaa
     3361 gaacttttgc tgtataagaa gaatctttta tcgctgcatg agaaaggcta tcagaaataa
     3421 ttgaaatttt atcttcacgg tttataccgg ttaaagaatc gggaatttga gataaaatat
     3481 tgttagtaac atcttcaatt cttaccaaaa atgtaactgt gagacctggt gtaggaattt
     3541 catcttctaa tttttgagca gtaaagccat tttttaaata attatgctct atggtactat
     3601 gctgttgtat ggcattaaat ccacaatgat gattagtaaa gactaatcca tcaacagaaa
     3661 ccattacacc tgtacagcca ttaccaaaaa taatcaccgc atcttttaag ctgatactgt
     3721 cgttattgta aatatctttg gccgaaaggg tacatcccat ttcatgcatc ttgttgatat
     3781 tgagtttttc aatgagagga agcatccaca tgccttcatc ggcttttgca acaatcagcg
     3841 aacaaaataa aagtatgaaa ataatgaatt gtttcataat tgatgaatat ttaatgagat
     3901 tttcaggaat caaaatgagt taccatctgc caaaaataat aagatttaaa gagaataaaa
     3961 aaccaatgct taataattaa aaaatgaaag cattggcaat tacaaaaatt gaaattattc
     4021 cacaatggtc gtccaaccat aaatatccgg ttcatcgcca tattgaatag cacgaatttt
     4081 attgtaaagt ttttcgcaaa ttacacctgg tttcccatca ggtgaaaata tgtatgattt
     4141 attttcttcg atatcgtcaa ttctctgaat cggactaatt actgctgctg ttccacaagc
     4201 tccagcttct tcaaaagtac ttaattcttc aagtggtacg ggacgttgtt ctaccttcat
     4261 tcccatatca cgagccaaaa ccattaaact tttattagta acagaaggca atatagaggt
     4321 agactttggt gtgatgtaag tattgttttt tattccaaag aaatttgctg ctccacattc
     4381 gtcaatatat tttttttctt ttgggtctaa ataaaacacc gctgaataac ccattgcatg
     4441 agctttttcg tcgggcacca aactcgcagc ataattaccg cccaccttat aagttcctgt
     4501 tccatgtgga gctgctctat catattcacg aaaaataaca aatggagtag ctttaaaacc
     4561 acctttaaaa taagctccta ctggtgtcac aaaaatgatg aattgatatt ctctggcagg
     4621 tttcacacca acttgggcac ttgtcccaat cataaacgga cgtatataca acgaagctcc
     4681 tgattcataa ggaggaacaa aacgttcatt caattttaca actttcaata ctgcttcttc
     4741 aaatttttca accggtaatt gtgccattac aataccagca caactcgatt gcattcgttt
     4801 ggcattttcg tccatacgga aaatacgaat tttcccatct ttccctcgga aagcttttaa
     4861 tccttcaaaa acttcttgtc cataatgaag acaagtagat gccatgtgca atgttaaata
     4921 ttcatcatca tgtacttcaa aatcacccca tttgccatct ttataggtgc atcgaagatt
     4981 gtaatcggtt ttaatatagc cgaatcctaa attgttccaa tcaatttcca ttttgtttga
     5041 aatattttat aatgttgtta gttataataa cttgtttagt attcttcatc aaaattactt
     5101 ctcagataaa atcaattgac tttcattgcc catgttaaag agcaaatcaa tcacgctcaa
     5161 acccttaaca aatccaaatt ttcccctgaa tacttgataa tagggagaat attcaatttt
     5221 cgattgtctc ttgggatgaa attcttctct caaatcaatc cttttgtcgg cgggaaatgg
     5281 ttcaaatttt tccgtaaagt ttatttgagt ttccaaatca agaagttcaa taattttatt
     5341 ttgaatttca agattgaaat cccacaaaaa taaccatttt tttttaaaaa aaggatataa
     5401 atcatcttca taatattcaa aaaaaggact tgaagaatag gcagaaacaa tcgctcgcca
     5461 atgttgtttt tgccaatcag aatgaacaga aattttaaca tctttgatag acggcttgtt
     5521 tccttccgat ttctccacag gaataatcaa gtccatcact ccattagcag taagaatttt
     5581 acaacgattc cgataggttt gtttgatata attttcctct ttttcaataa aaatttctga
     5641 actatttgcc agcactttgt aatattcaac aggtgctaaa tatgctaatg ataaaatggc
     5701 aggcattgaa ttctcactct tccattgact aaggattttt gcttgattct tatactgaat
     5761 tttttttaac gaaaaatcaa aagtatcttt atcgtgttgc atttttaaaa aacctcttcc
     5821 atctaacttt tccattgaac cacgacttgt ctttatccaa cgacaaccaa acaaagacag
     5881 gttgacccac tatgtgatct tcggggacaa atccccaata acgtgaatca gccgaattat
     5941 gtctgttgtc acccatcatc cagtaataat tcattttgaa ttggtaagtt gtggcaggct
     6001 tatcattgat ataaaatttg ccttccttca tttcaagctt atttttttca tatacccgaa
     6061 ttactcgctc ataaatggga taattgtatg catttaatac tagcttggct ccttttcgag
     6121 ggatataaat tggtccataa ttatcacgtg tccaattttt accgccacct aatggaaaaa
     6181 cttctccacc atattggtcg ggttcaacaa tgatttttgt aacacctgaa acattcctca
     6241 acttgttata ggcttcttct gttaaaggaa aatgataaat gggaagctca ggatctaaac
     6301 ccaatgaaat aatttcacct ggattttgca tggtatttaa caataatcga tcatctttac
     6361 ttatatgcaa tttacttaat aattcgtctg aaagccttcg cccatcagtt tgcacgagat
     6421 aattaaattg gataccgggg aatttttcct cttttttacc attaacataa acttgattat
     6481 tcctgatttc cacccagtca ccagccactg ccacgcaacg ttttacataa ttttctctcc
     6541 gatcgacggg acgatatacg atctctccaa aaatatcttt ccgactgtga actacctctc
     6601 tgccataata ataacaaagc tgataataat caggattttg ttgctttaag gcaacggtat
     6661 cgccagttgg gaaattaaat accacaatat catttcgggc aatggtgtcg agtcctttta
     6721 aacgtttata atcccattga ggcttttcga gatatgattt tgtgttaatg attggaaaag
     6781 tgttttgtac caaaggaaat gataggggag taaacggtac tcgtggtcca taatttactt
     6841 tgctaacaaa aagaaaatca cccaccaata atgttttttc gagagaagaa ctggggattt
     6901 gataattttg gaataaaaat aaattgataa aatatactgc tactaatgca aatatgatag
     6961 catctaccca gcttaaaaaa gaacggagac ttttattttt tactttcttc caccaggtcc
     7021 acggaataaa tttggtaatg taaatgtcaa aaataaaagg tagcagtaac aaccaccaga
     7081 aattatttac ccacgcaata aacaaaatat aaataagtga ccatatacca gcttttaccc
     7141 attgtttaag aggctgatga aaacgtgtcc taaatttttg agaaaccata aaaaattttt
     7201 attaattaca aatactccta attaaaacta cttaaaaaaa cagcaataaa tctttatgtt
     7261 aaaatatatt tttcaaaatg aataaccctg aggcaagcct cgaagaattc ttttgattaa
     7321 aaaggaaaaa ctaaaatttc agaaaatcag ccatagtaaa ccaacctttc tttcctgcca
     7381 aaaactctgc cgccaacact gctccaacag caaaaccatc acgatttttc gcattgtgcg
     7441 ttatttcaat ggtgtcaatt tgcgattcat aacgaacggt atgaatgcct ggcacatcat
     7501 cagtcctaat agattgaata gctatttcat cactattttt ctgaacatct tttacccatt
     7561 ttttctttcg gttcagtttt tcaataattc cttcagccag agtaatagct gtaccacttg
     7621 gtacatctaa tttttgggtg tgatgtattt cggtaatgtt aacatcatat tcagaaaatt
     7681 gattcatgat gtttgacaga tatttattga cggcaagcaa aatgttgact cccaaactga
     7741 aattggaaga ccacaaaaaa gtataaccat tttcctcaac ttcctttttc agcaaaggca
     7801 attcatcatt gatgccagtt gtgcctgaaa ctactggaat attatgttca aaagctttct
     7861 tataattatc caatgctgat ttaaaattag taaattcaat agcaacatcg gcttgcttaa
     7921 aattttctga atcgaaatct tccagattgt ttacatcaat aatggaaaca attttatgac
     7981 ctcgctctcg ggcaattttt tcaatggttt tccccatttt gccatatcca atcaatgcta
     8041 acttcatatt aaaaattgat atttttacat tttatattaa aagtaaatca ttaattttca
     8101 aatttttagt gttagttagt atttacatag tcctagtgta caggtcttca taaaccgtta
     8161 cttaaaaaat ctaaacactc aaaaatttct tctttactgg cagtttgatt aagttgtatt
     8221 tgaccaatat ctgacagtaa agtaaaatta atttctttcg atcgattttt tttatcgtgc
     8281 gtcattaatt cataaatctt ttcataattt tcgcaaggaa tgaaaaaaat accataatat
     8341 tcttttatca aatattttaa tttcaacaaa tcttcttgag gaaaattaaa tctgataaca
     8401 gaaagatata attcacacaa caatccccac attacagcat aaccatgcaa aacaggttta
     8461 ttagaaagaa gagaaaaact ttcgaaagca tgaccaaaag tgtgacccaa attcaaagct
     8521 tttctcaaat tctgttcctt cggatctttt tctacaatag cttctttaat atgaacactt
     8581 ttttctactt tctgctttaa ataatcaaaa tcaatattgt ctaaatcaat tgttaatata
     8641 tcattccatt ctttacgtga atccagtaaa gcatgtttta ccatttctgc aaaaccagaa
     8701 cgaaaatttt tatcatccag cgttttaaaa aagtcaacat taatcaggac tttttcagca
     8761 ggagcaaaca caccaatttc atttttcaat cctaaaaagt tgatccctgt ttttccacct
     8821 gtcgcagcgt ctactgctcc aagcaaagtg gttggaatat tcaaatatcg aattcctcgt
     8881 ttgaaagtag ctgcagtaaa accacctata tcggttacca tgccaccgcc aacattgatc
     8941 attaacgatt gtcgtgtagc accatgctcg ctcaaatatt gccagatttg aatagctgtt
     9001 tcaatgtttt tatgctcatc acctgctgga atagaaacaa cgtatgaatt ttgtaattta
     9061 ttgattttca ttatagtagg caaacaaaga cgttgcgtat tttcatctac aagtataaaa
     9121 atactctctg actcttgatt tccgataact tcacttaatt ctgaaacgaa atcattacaa
     9181 attatagttg aaccttgaag catttttcgg gttttttgtc cacaaagata cttttttatt
     9241 taaaattaac ctctaaaaat ttttcagaaa gtcattgttt ttacgaagat tatatttttt
     9301 ttctgttttt gtattaaaaa tatttaaata tttaactata tatgaataaa tgttcatact
     9361 tttgtacttg aatttaaata agaaaagtgt tgaaaaatac aaaacaaata gaagaagctg
     9421 cttacttgct taaagcaata gccaattgca ctcgtttggg tattattcta ttactttcag
     9481 aaaaagatga attaaacgtg tcgcaacttc aggaagaatt aaattgtgaa cagtcgttgc
     9541 tttctcatca tctgacagat atgagagcaa aagggatatt aaattgcagg cgagaaagca
     9601 aaaattgtta ttattccgtg aaaaacaaaa agatagtgca agtaattgaa tgtatcctct
     9661 cctgtcaaaa tcaaaattaa aaagaaagaa aaagctaagg acgtttttaa aaaaatactt
     9721 tatggaacaa ttggcgattt ttattgtttt ttatctttcc gcagaaagaa aaaacaaaaa
     9781 ataaaaatgt tattaataat tgtttgcaat tgctttatta gtagcaaaaa a
//
LOCUS       DAOSAS010000063         9791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4546_length_9791_cov_13.700082, whole genome shotgun sequence.
ACCESSION   DAOSAS010000063 DAOSAS010000000
VERSION     DAOSAS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9791
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4546_length_9791_cov_13.700082"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..372)
                     /gene="trxA"
                     /locus_tag="PLR72_04230"
     CDS             complement(55..372)
                     /gene="trxA"
                     /locus_tag="PLR72_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOK36445.1"
                     /translation="MALEFTDENIKEIINNGKPVVIDFWAEWCGPCRMMSPIIEELAH
                     EYEGKVEIGKLNVDENSETTEEYGIMNIPTLLFFKNGQIVDKQVGATQKSVLVKKIEA
                     LLS"
     gene            complement(514..4305)
                     /gene="dnaE"
                     /locus_tag="PLR72_04235"
     CDS             complement(514..4305)
                     /gene="dnaE"
                     /locus_tag="PLR72_04235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOK36446.1"
                     /translation="MNSFVHLHVHSHYSILDGMSSIPDLIEKAIAHNMPALALTDHGN
                     MFGIKEFYNYVKKKNQPILDKIASLTQQFQNIQLTDEEKKEIQLQIDREKKNLFKPII
                     GCETYVAHRSRHLKEIPEDRSGYHLILLAKNKTGYHNLCKIITLAYTEGMYYRPRIDK
                     ELLEKYREGLIATSACLGGEIHKKIESGNLAEAEEAILWYKKIFGDDFYLELQRHQTD
                     KPGADVTTYEKQKIQNAELIKLARKTNTKLIATNDVHFVEEEHADAHERLICLSTGTD
                     LDDPQRMRYTKQEWLKTPQEMAAIFEDIPEALNNTLEVAEKVEFYDIDSAPVMPLFPI
                     PEDFATEAEYRSKFTEEMLRQEFEESFDRLGGYNKVVRIKLEADYLTKLTLAGAEERY
                     GKNLPPEIEDRINYELKVIKTMGFPGYFLIVQDFIQAARDMDVAVGPGRGSAAGSVVA
                     YCLKITDIDPLKYDLLFERFLNPDRISMPDIDIDFDDDGRNQVLHWVTEKYGKNRVAH
                     IITFGTMAAKMAIKDVARVQKLPLSEAERLTKLIPDRLPDTPDGKKLKVNLSNCFEFV
                     PEIQVELDSSNKNIVETLKYAEMLEGTVRQTGVHACGVIIGADDLSNFIPLSTAEDKD
                     TKEEILVTQYEGSVIEEVGLIKMDFLGLKTLSIIKDTIENIKKSKGIEIDISSIPIDD
                     PETYQLFSKGQTIGTFQFESPGMQKYLQALQPTQFEDLIAMNALYRPGPMQYIPQFIK
                     RKHGLEKIEYPFPVMEKRLKETYGITIYQEQVMLLSVDLAGFTRSEADELRKAMGKKL
                     NNKMEALKEKFIIGCQKNGYGPLEKIEQIWNDWIEFSNYAFNKSHATCYSLIAYQTAY
                     LKAHYPAEFMAANLSRNKDNIDEIRKFMDECKNMGINVLGPDINESDLTFSVNEKGDI
                     RFGLGGIKGVGEGAVTNIINERNSHGKFKSVFDFVERVNLSACNKRTFESLALAGAFD
                     SLNDIQREQFFVENSKGEQVLESLIKYGNKFQIDKSMNHNTLFGLFDEIEIAKPEIPF
                     APQWSSLVRLNKEKELIGIYLSAHPLDEYEFEINYLCNTTTSDLKNLNELTDKTLKLA
                     GIVTSIRHATSRTGKPYGVFTLEDYNGSYEFVLYGNNYIDFQKYMIKDLYLFIQATVQ
                     EKYADSNYRKKSNFTSSELELKIQKIDLLQDIKNKIINSLTLMIPLQELTNDLAQQLT
                     DVVLQNKGNINLYIQVFDDQNVANKINFFARRHKIEMNSTVFQFLKEIKKNGKFDFQV
                     N"
     gene            complement(4553..5077)
                     /locus_tag="PLR72_04240"
     CDS             complement(4553..5077)
                     /locus_tag="PLR72_04240"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOK36447.1"
                     /translation="MERIFLIGYMGAGKSTVGELLAQALGYEFIDMDHEIEKRTQQTI
                     SNIFAEKGENEFRQLEYNCLHEIAQQNRVVISTGGGAPCFFNNLSFMKENGLTVYLKV
                     PAEQLKEKLEMSQHDKRPLISGKKGNDLLQYIKKGLAEREPFYQQATLVVTGTYEEML
                     NQILNYLSMHQKNN"
     gene            complement(5209..5748)
                     /locus_tag="PLR72_04245"
     CDS             complement(5209..5748)
                     /locus_tag="PLR72_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36448.1"
                     /translation="MKKQFFILLGCVAILFTSCLKNEESNYTPTISFLTYPITNHNDS
                     LNIKLIDPSGTYQLDTIEVGDTVTFLLGLNGYTNNLTSFYVQHTDSSATKIDFPPLEN
                     LNNIFSEPLSDYESGKFIFAYNYSSIYFSFNYIAKKPSNNAAIIFTLSSDAQFSEGSG
                     YNTVSFRLKTPIKQKISIQ"
     gene            complement(5799..5871)
                     /locus_tag="PLR72_04250"
     tRNA            complement(5799..5871)
                     /locus_tag="PLR72_04250"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5836..5838),aa:Pro,seq:cgg)
     gene            complement(6256..6329)
                     /locus_tag="PLR72_04255"
     tRNA            complement(6256..6329)
                     /locus_tag="PLR72_04255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6293..6295),aa:Arg,seq:acg)
     gene            complement(6415..8547)
                     /gene="rnr"
                     /locus_tag="PLR72_04260"
     CDS             complement(6415..8547)
                     /gene="rnr"
                     /locus_tag="PLR72_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOK36449.1"
                     /translation="MSKKRKTSSHLPKKELIHYITTIFNENPERTFNYRQIAAALNIH
                     SSSQRIYIRELLEELAEEDFLIEVSQGKFKANVRNKYVEGIVTRTGIKTYVIPDDGGD
                     KIFIPERKTNHALPNDRVKVLLYAHRHNQPLEGEVIEILERSQNTFVGVLEVSDNFAF
                     LVVDNHIMSNDIFIPKNKLKNGKSGQKAVVKIVDWTSEMKSPIGEVIDILGNKGENNT
                     EMHAILAEFGLPYQYPDEVEKAANKIDARITSQEIARRIDMRDVVTFTIDPREAKDFD
                     DALSIHQLENGLWEVGVHIADVTHYVQPNSMIDQEAQKRGTSIYLVDRTIPMLPERLS
                     NNICSLLPNEDRLTYSVIFQLNDKAEVINYQIARTVIRSKQRFTYEEVQKIIESGEGN
                     YKNEILKLNELAQILRKNRLNNGAIDFERVEVQFEIDKKGRPLSVFFKEQKEANQLIE
                     EFMLLANKTVATHIGKPQNGKKTKTFVYRIHESPDAQKLKDFASFIKKFGYHFKTSLP
                     KSKISNEMNKLLNDVQGKKEQNLIETLAIRSMAKAIYSTKNVGHYGLAFDYYTHFTSP
                     IRRYPDMMVHRLLTRYLANGRSVNTEEWEQYCEHSSDMEQLAAKAERESIKYKQVEFM
                     SERIGQIYNGIISGVTEWGIYVELIENKCEGMIPIRNLDDDYYIFDEKNYCLVGRHHQ
                     KRYCLGDEIKIKVVKANLDRKELDFALA"
     gene            8753..9637
                     /locus_tag="PLR72_04265"
     CDS             8753..9637
                     /locus_tag="PLR72_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOK36450.1"
                     /translation="MKKKIGFIINPAAGNKNKNFIPDYIQQLLDLQLFKPEIVFTKYA
                     GEGSLLAKEFVNDGFSNVVAVGGDGTVNEIACSLIHTNTSLGILPIGSGNGLARHLKI
                     PLNIPKAIQLLNNSESINIDYGIVNQRLFFCTCGTGFDAYVATEFAKGRKRGIKAYFE
                     KVLKGYFSYRSQNYRLQGKNIDVEIKAFLLTFANASQWGNNAYIAPKASLQDGKMDIS
                     IMSNFPIFALPSIALQLFTKTISNDLFMTTLRTDEITVYRDTPGPFHYDGEPYEEAKE
                     IKIKMIPAGLKVLVRKRF"
BASE COUNT         3145 a         1653 c         1497 g         3496 t
ORIGIN      
        1 agaaaaagtg cggaaaacag atggagaaat aagttataaa ctttttccga aatttcagga
       61 aagaagtgct tcaatttttt ttaccagaac acttttttgt gttgcaccaa cctgtttatc
      121 aacaatttga ccatttttaa aaaacaaaag tgtcggaata ttcataatgc catattcctc
      181 agttgtctca ctattttcat ccacattcag tttccctatt tctacttttc cttcatattc
      241 atgagccaac tcttcaatga taggtgacat catacgacag ggaccacacc actctgccca
      301 aaaatcaata actactggtt taccattatt aataatttct ttaatatttt cgtctgtaaa
      361 ttctaatgcc ataattttat aattttaatt gtttgatcaa taatttatta acgaatttct
      421 aaataaattg tttttaaaac tctattatca tttttatttt attgggaaaa acttaatgaa
      481 ctcccagatt gaaattacct catctgacaa aatttaattg acttggaaat cgaattttcc
      541 atttttttta atttctttca aaaattgaaa aactgtggaa ttcatttcaa ttttatgtcg
      601 tcttgcaaag aaattgattt tattagcgac attctgatca tcaaatactt ggatatataa
      661 attaatattt cctttatttt gaagaacaac atctgtgagt tgctgagcca gatcatttgt
      721 caattcttgt aatggaatca ttaaagtgag tgaatttatt attttatttt ttatatcttg
      781 taataaatca attttttgta tttttaattc aagttctgat gaagtaaaat tgcttttttt
      841 acggtaattg ctatccgcat atttttcctg aacggtagcc tgaataaaaa gatacaaatc
      901 ctttatcata tatttttgaa aatcaatgta gttattacca taaagtacaa attcatacga
      961 gccgttataa tcttctaaag taaaaacacc ataaggcttt cctgttctgg aagtagcatg
     1021 acgaatactg gttacaattc ctgctaattt caacgtttta tctgtcagtt cgttaagatt
     1081 ttttaaatcg ctggttgttg tattgcaaag ataattaatt tcaaattcat attcatcgag
     1141 aggatgagca gaaagatata ttccaattaa ttctttttct ttatttaaac ggacaagcga
     1201 cgaccattga ggagcaaaag gaatttcggg tttggcaatt tcaatctcat caaaaagtcc
     1261 aaataaagta ttatgattca ttgatttatc tatctgaaat ttgttcccat atttaataag
     1321 gctttccaaa acttgttcgc ctttactatt ctcaacaaaa aactgttctc gctgaatatc
     1381 atttaagcta tcaaaagctc ctgccaaggc aagactttca aaagttcgct tattgcaagc
     1441 agaaagattg acgcgttcta caaagtcaaa aacactttta aattttccat gtgaatttcg
     1501 ctcattgatg atattagtga cagcaccttc gccaacgcct tttataccac ccagtccaaa
     1561 acgaatatct cctttttcat ttacagaaaa agtcaaatca ctttcgttta tatcaggacc
     1621 cagtacgttg atacccatat ttttgcattc atccatgaat tttcttatct catcaatgtt
     1681 atctttattt cttgacaggt tggctgccat aaattctgct ggataatgtg ctttcaaata
     1741 agctgtctga taagcaatca acgaataaca ggtagcatgt gatttgttaa aagcatagtt
     1801 tgaaaattct atccagtcat tccatatttg ttctattttt tctaatggtc cataaccatt
     1861 tttttgacaa ccgataatga atttttcttt caaggcttcc attttgttgt tcaacttttt
     1921 gcccatagct ttacgaagct catcagcttc acttcgtgta aaacctgcta aatcaacact
     1981 caaaagcatt acctgttcct gataaatggt gataccatac gtttctttta gccttttttc
     2041 catcactgga aaaggatatt caattttttc gagaccatgt tttcttttga tgaattgagg
     2101 aatatattgc ataggtccag gccgatataa tgcattcatg gcaattaaat cttcaaactg
     2161 agtaggttgc aaggcttgca agtatttttg cataccagga gattcaaatt gaaaagttcc
     2221 aattgtttgt cctttactga ataattgata tgtttcagga tcatcaatag gaatagaaga
     2281 aatatctatc tcaatgcctt ttgatttttt tatattttct atcgtatctt tgatgatgga
     2341 aagagttttc aatcccaaga agtccatttt tatcaatcct acttcttcaa tcactgaacc
     2401 ttcatattga gtgactaaaa tttcttcctt tgtatcttta tcttcggctg tacttaaagg
     2461 aataaaatta cttaaatcgt cggctccaat aatcacgccg caagcatgaa ctcctgtctg
     2521 acgaacagtg ccttcgagca tttccgcata tttaagagtt tctacaatat ttttattgga
     2581 ggaatccaac tccacttgaa tttctggaac gaactcaaaa caattggata agtttacttt
     2641 taattttttc ccatcaggag tatcaggtaa tctgtcagga attaattttg tcagacgttc
     2701 agcttccgaa agaggtaatt tctggacacg tgccacatct ttaatagcca tttttgccgc
     2761 catcgttcca aaagtaataa tatgagccac tctgtttttt ccatattttt cagtaaccca
     2821 atgtaatacc tgatttcttc catcgtcatc aaaatcaata tcaatatcgg gcatagaaat
     2881 tcggtcaggg ttaagaaaac gttcaaaaag caaatcgtat ttcagcggat caatatcggt
     2941 tattttcaaa caataagcta ctaccgaacc tgctgctgaa cctcttcctg gaccaactgc
     3001 aacgtccata tctcttgctg cctgaataaa atcttgtaca ataagaaaat aaccaggaaa
     3061 ccccattgtt tttattactt ttaattcata attgatacga tcttcaattt ctggaggtaa
     3121 attttttcca taacgttcct ctgcacctgc cagcgtaagt ttggttaaat aatccgcttc
     3181 cagtttgata cgcactactt tattatagcc acccaaacga tcaaaacttt cttcaaattc
     3241 ctgacgtaac atttcttcag taaatttgga acgatattca gcttcggtag caaaatcttc
     3301 tggaataggg aaaagaggca ttactggtgc tgaatcaata tcataaaatt ctactttttc
     3361 agcaacttcc aaagtattgt tgagtgcttc gggaatatct tcaaaaatag ctgccatttc
     3421 ttgtggcgtt ttaagccatt cttgttttgt ataacgcatc cgttgaggat cgtctaaatc
     3481 tgtacccgtg cttagacaaa tcaaacgctc gtgtgcatca gcatgttctt cttctacaaa
     3541 atgcacatca ttggtggcta taagtttggt attggttttt cgagccaatt tgattaattc
     3601 agcattttgg attttctgtt tttcataagt ggtaacatcg gcaccgggtt tatctgtttg
     3661 atgacgttgt aattccaaat aaaaatcatc tccaaaaatt tttttgtacc agagaatggc
     3721 ttcttcagct tctgcaagat ttccactttc tattttttta tggatttctc cacccagaca
     3781 agccgacgtt gcaattaatc cttctctata tttttcaaga agttctttat caattcgggg
     3841 acgataatac attccttctg tataagctaa tgttatgatt ttacaaagat tgtgataacc
     3901 agttttgttt tttgccagca gaattaaatg ataaccactt ctatcttcag gtatttcttt
     3961 taaatgccga ctacgatgag ctacataggt ttcacatcca ataataggtt taaacaaatt
     4021 tttcttttcc cgatctattt gaagttgaat ttcttttttt tcttcgtctg tgagttgaat
     4081 gttttgaaat tgttgagtta aagaggcaat tttatcaaga ataggctgat tttttttctt
     4141 tacataattg taaaattcct tgattccaaa catattgcca tgatctgtta aagcaagggc
     4201 aggcatattg tgggctattg ccttttcaat caaatcaggt atagatgaca tcccatcaag
     4261 aatggaataa tgagaatgaa catgaagatg aacaaaagaa ttcatgataa atcaaactaa
     4321 tgggtctttg aatatgaata aaatagctaa aaaatttttg aaggataaag ttaataaaaa
     4381 aatgcatcaa aaaaatgtaa aaaaattgaa agaggatgaa ttattttagt tctcgattga
     4441 ccatttcaga aatagatgta aatactcctc attttccttc tgaaaaagtt gaaaaagcaa
     4501 ctgtttgtta aaacagcaac atgtgaaaag ggaagtcttt tacttcaatt ttttaattgt
     4561 ttttttgatg catggaaaga taattcagga tttggtttaa catttcttcg taggtacctg
     4621 ttactacgag agtggcttgt tggtaaaaag gttctctttc agccagccct tttttaatat
     4681 attgcaacaa atcattacct tttttgccag aaataagagg acgtttgtca tgttgtgaca
     4741 tctcaagttt ttctttaagt tgttctgctg gcacttttaa ataaacggtc aaaccattct
     4801 ccttcataaa ggacaaatta ttaaaaaagc aaggtgcgcc accaccagtt gaaataacaa
     4861 cgcgattttg ctgagctatt tcatgtaaac aattatattc taattgtctg aattcatttt
     4921 cacctttctc agcaaaaata ttagagatag tttgttgtgt tcttttttca atttcatgat
     4981 ccatatcaat aaattcatag ccaagagctt gtgctaaaag ttcacccaca gtagattttc
     5041 cagcgcccat gtaaccgatt aaaaaaattc gttccatata aaaaaattgc ttcaaaaaaa
     5101 tgttagaaaa caaaaactta caaaattaac ataattaaaa taaagttata aaaaaaggac
     5161 atttttcttt tttgaaaaag tcctttttaa acatcctcaa aaaactaatt attggatact
     5221 aattttttgc tttatgggag tttttaatct gaaagaaaca gtattatatc cacttccttc
     5281 agaaaattgt gcatcagaag aaagagtaaa aataattgca gcattattgc ttggtttttt
     5341 agctatataa ttaaaagaaa aatatattga actataatta tatgcaaaaa taaatttccc
     5401 actttcataa tctgaaaggg gttcagaaaa aatattattc agattttcaa gtggtggaaa
     5461 gtctatttta gtagcactgc tgtcagtgtg ttgcacatag aaggaggtta aattattcgt
     5521 ataaccattt aacccaagca aaaatgtaac tgtatcgcct acttcgatag tatcgagttg
     5581 ataagtacca ctcggatcaa ttaatttaat atttaaggag tcattgtgat tggtaattgg
     5641 ataagttaaa aaactaatag taggagtata atttgactct tcatttttta gacaggatgt
     5701 aaataaaata gccacacatc ccaataaaat aaaaaattgt tttttcataa taaatttatt
     5761 tttagggaaa taataatttt tgataaaaaa cgctaatgtc ggggtgacaa gactcgaacc
     5821 tgcgacctct acgtcccgaa cgtagcgcgc tgccaactgc gccacacccc gaaaaaagaa
     5881 ttttgataca aatttacacg attttattga taataaaaag gaattttctc gtaagaaaga
     5941 ataaaagcta cctgttgaaa atgtgaatgg gagaattgtg ttgaatatta cacttagaga
     6001 atgattaaaa gataactttc tgcaacctga atagctcttg ggtaattttc gcaaagctga
     6061 gcttctctaa tttcggccat tttcgctctt ggagaatttt tatcaaccaa tggaatataa
     6121 ccacaataat agaaaagaga atttttctat aacgactata gtaaggttcg gaagaagaaa
     6181 aatcagacat attcatattt aaggttgata aaaaataaaa aagagacctt ttgtttttta
     6241 aagtctcttt atttgcgacc ccgacaggac tcaaacctgt aaccttctga tccgtagtca
     6301 gatgctctat tcaattaagc tacggggccc ctttttttgt gcttgcaaag atagggttat
     6361 tttttaaaaa tagcaacctt cctttttaaa aaaatcagaa aaactacata agcatcaagc
     6421 taaagcgaaa tctaattctt ttctgtccag attggctttt actactttta tttttatctc
     6481 atcgcctaaa cagtaacgtt tttgatgatg tcttcccaca aggcaataat ttttttcgtc
     6541 aaaaatgtaa taatcatcat ccagattgcg aattggaatc attccttcac atttattttc
     6601 tattaactct acataaatgc cccattcagt aactcctgaa ataattccat tataaatttg
     6661 tcctattctt tccgacataa attcaacctg tttgtattta atggattctc tttcggcttt
     6721 agcagccagt tgctccatat cggaactgtg ctcacaatat tgttcccatt cttctgtgtt
     6781 cacactgcga ccatttgcca gatagcgagt taataaacga tgcaccatca tatcaggata
     6841 tctacgaata ggtgaagtga aatgagtata ataatcgaaa gccagtccat aatgacctac
     6901 attttttgta gaataaatgg cttttgccat agaacgaata gctaacgttt caattaaatt
     6961 ttgttccttt tttccttgta catcatttaa taatttattc atttcatttg aaattttaga
     7021 tttaggcaag gaagttttga agtggtaacc aaatttttta ataaagctgg caaaatcttt
     7081 cagtttttgt gcatcgggcg attcgtgaat tctatacaca aatgtcttag ttttttttcc
     7141 attctgtggt tttccaatgt gggttgccac tgttttgttt gccagtagca taaattcttc
     7201 aattaactgg ttggcttctt tttgttcttt aaagaatact gaaagaggtc ttcctttttt
     7261 gtcaatttca aattgtactt ctacccgttc aaaatcaatt gcaccattat ttaaacgatt
     7321 ttttcgaaga atttgtgcta attcatttaa ttttaaaatt tcatttttat agtttccttc
     7381 tcccgattca ataatctttt gaacttcttc gtaagtaaag cgttgtttgc tacgtatcac
     7441 agtacgagca atttgatagt ttatcacttc cgccttgtca ttcagttgaa aaattacgga
     7501 ataagttaac ctatcttcgt tcggtaacaa ggaacaaata ttattcgaaa gtcgttcagg
     7561 gagcatagga attgttctat caaccagata gatagaagta ccgcgttttt gagcttcctg
     7621 atcaatcata ctgtttggtt gaacataatg tgtgacgtca gcaatatgta caccaacttc
     7681 ccatagtcca ttttctaatt gatggataga taaagcatcg tcaaaatctt tagcctcacg
     7741 aggatcgatg gtgaaggtta ccacatcacg catatctatt cgtcgagcaa tttcttgtga
     7801 agttattctt gcatcaattt tattagctgc tttctcaact tcatcaggat attgataagg
     7861 caaaccaaac tcggctaaaa tagcatgcat ttctgtgttg ttttcgcctt tattacctaa
     7921 tatgtcaata acttcaccga ttggactttt catttctgat gtccaatcaa cgatttttac
     7981 tactgctttt tgtccacttt tgccattttt caatttattt tttggaataa atatatcatt
     8041 actcataata tgattatcta ccaccaggaa ggcaaaatta tcggaaactt ccagcacacc
     8101 cacaaaagta ttctgagaac gttctaaaat ttcaattact tctccctcaa gcggttgatt
     8161 atgtctgtga gcatatagaa gaacttttac gcggtcgttt ggtaatgcat gattagtttt
     8221 gcgttcggga ataaagattt tgtctccccc atcgtctggt atcacatatg ttttaatgcc
     8281 tgtacgcgta acaatgcctt ctacatattt attacgtaca ttggctttaa attttccttg
     8341 cgaaacttca attaagaaat cttcttcagc taattcttct aataattcgc ggatatatat
     8401 gcgttgtgaa gaagaatgaa tattcaaagc agccgcaatt tgtcggtaat tgaatgttct
     8461 ttcagggttt tcattaaaaa tagtggtaat atagtgaatt agttcttttt taggtaaatg
     8521 actacttgtt tttctttttt tggacataaa aatgagttat aaaaaataat taaataagag
     8581 aatgttcaaa tatgttttta ttaaaataag tatgaatcaa cagattaata aaattttgca
     8641 aagttacaac aaaataatta atggaatagt ttattacttt gaaaatgatt ttttcgttcc
     8701 caaaaattat ttgtaatttc actaccaatt ttgggatttt tatgaaatca cgatgaaaaa
     8761 gaaaattggt tttattatta accctgcagc cggaaataaa aataaaaact ttattcccga
     8821 ttatattcag caattattag acttgcagct tttcaaacca gaaattgttt ttacaaaata
     8881 tgctggtgaa ggttcattgt tagcaaaaga atttgtgaat gatggttttt ctaatgtagt
     8941 ggctgttggt ggggatggta cagtaaatga aatagcatgc tcactgatac ataccaacac
     9001 ttcgctgggt attcttccaa taggttctgg caatgggctg gctcgtcacc tcaaaattcc
     9061 cctgaatatt cctaaagcta tccaactttt gaataattca gaatctatta atattgatta
     9121 tggaatagta aatcagcgat tatttttttg tacctgtggc actggattcg atgcttatgt
     9181 agcaacagaa tttgcaaaag gcagaaaacg aggaataaaa gcttattttg aaaaagtttt
     9241 gaagggatat tttagttatc gttcacaaaa ttatcgttta caaggtaaaa atattgatgt
     9301 agaaatcaaa gctttcttat taacatttgc taatgcttct caatggggta ataatgctta
     9361 tattgcacca aaagcaagcc tgcaagatgg taaaatggat atttccatta tgagtaattt
     9421 cccgattttt gccttgcctt ccattgcact tcaacttttt actaaaacta tttctaatga
     9481 tttgtttatg acaactttac gaacggatga aattactgtt tatcgtgata ctcctggacc
     9541 ttttcattat gatggcgaac cttatgaaga agcaaaagaa ataaaaatta aaatgattcc
     9601 agcaggctta aaagttttgg tgagaaaacg tttttaaatc acttaatttt cgtgtatttt
     9661 agctttaagt ttattgtgaa tcttgaagtt aacaatttaa agatccacaa gaaagctaaa
     9721 taaggtaata aggttataat taaggagata tttcaaagtt attagggttc aatgttttta
     9781 tataaggttt t
//
LOCUS       DAOSAS010000064         9713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4586_length_9713_cov_13.320046, whole genome shotgun sequence.
ACCESSION   DAOSAS010000064 DAOSAS010000000
VERSION     DAOSAS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4586_length_9713_cov_13.320046"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="PLR72_04270"
     CDS             <1..1310
                     /locus_tag="PLR72_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36451.1"
                     /translation="IFKQKNIMKPIQLIIIFLSLLIPTLGYSQKETKVIDDIYYSPND
                     KTPTYSSKNEQKPTYKNGAKEIIFREHSDSIKENSTLSMTKEENDSLLNDTIHEEGYY
                     LHGFKGSSSDYEYAERIRRFHNPKFTIHISDPDYMDLYFLDDYNWNVYIEGGYAWVTP
                     TWTNPLWFDYYYRPFMYNSWYWRWSLYYPSYSYWYSPWYYPWHAWYYDPWYYSPWYYG
                     YYRHPYYGGYYHYKTINPSHNEANRRQITNFGNERRVASSSSSAMSTNSDNVSQRNGR
                     SNNPYTVITGTGGRSSNTTGMTTPSNAGNSSSEQTRRGAVLNNQNQSERTYNSANTRS
                     SSSSYANSENSRRSTSVNTQPRTSNTTNNSSGVRANTRSTYSAPSRSSSGESNRSGSS
                     YSSGSSSGSSTRSSGSSNSSGGSQSSGGSSSGSSRNSSGGGRR"
     gene            1354..2904
                     /locus_tag="PLR72_04275"
     CDS             1354..2904
                     /locus_tag="PLR72_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36452.1"
                     /translation="MKKNIGVWVLLFCSNLIVAQSELDIVKFVQPDINGTARYMSMAG
                     AFGALGGDVSAIKDNPAGLGIYQHSETVGTLNFLFQNNDNSWYGVNSSDNLYKLKANN
                     FSIVIASPTKQSKKGIKSGLQNSNFSFSFQQVKNFNRQTTIQSGESPSSITDYMAYFT
                     DGIPEADLQSSENYHPFNNQNIPWLSVLAYEGYLINPTSNNSWGSILNAGEKVIPSYY
                     LHESGHINKYSFSWAGNFSHMFYLGFTGNIQTIDYNAVTEYEETFGEGGGMNLNNTID
                     ASGSGFNLEMGIIFRPVDNFRIGLSFQSPTVFSISEDYTSLLNFNTESQGYISTPTGY
                     NSYKLQTPAVFDASAAYIFGKKGLISVEYNYTDLKTTKLMDKYGSTKDFKDENAGMKS
                     AVDAVSTIKIGGECRLTDNFSVRAGYANMSEATQKNAEKFMRINTIRTDTEYFLHNGT
                     RYLTAGFGYHENSWYIDLAYMNKLTKEKFYPYNSIRANEMVDCPIGEPAEVKTINNNL
                     VITLGLKF"
     gene            3111..4688
                     /locus_tag="PLR72_04280"
     CDS             3111..4688
                     /locus_tag="PLR72_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOK36453.1"
                     /translation="MNFKDEINRRRTFAIISHPDAGKTTLTEKLLLFGGAIHVAGAVK
                     SNKIKRTATSDWMEIEKQRGISVATSVMGFEYRNYKINILDTPGHQDFAEDTYRTLTA
                     VDSVIIVIDSAKGVEEQTRKLINVCRMRNTPIIVFINKMDREGRDAFDLIDELEKELQ
                     MEVCPLTWPIGQGISFKGVYNRYYNNLQLFTPNKQVVGSSIDISDVCSTKLEKCVGEK
                     EAQQLRDELKLISGIYSKFDIEKYLGGELAPIFFGSALNNFGVKELLDCFVEIAPSPR
                     PVEAMERKVNPDEEVFTGFVFKIHANMDPHHRSCIAFVKVCSGKFERNVNYKHVRKNK
                     IIRFSNPTAFMAQKKQIVDEAYAGDIVGIPDNGNFKIGDTLTSGEELHFKGLPSFSPE
                     MFKYLENSDPMKAKQLEKGIQQLMDEGVAQLFINQHNSRKIVGTVGELQFEVIQYRLL
                     HEYNAQCRWETLPLYKACWIESENKEELENFKKRKDQFLAKDKEGRDVFMADSPYILQ
                     MAQNDFKNIKFHFTSEF"
     gene            4807..5316
                     /locus_tag="PLR72_04285"
     CDS             4807..5316
                     /locus_tag="PLR72_04285"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOK36454.1"
                     /translation="MTSNIFKSSMIYGLFMGILFAANFFISLPQGIIFRLLSFLIIIL
                     IIYAMYRFSVRYRDNECGGYISYGKAFSFILLTFFFASIISSIFKFFYFQFINPDYLT
                     NLLQESLKIIDSMQLPVNEEFYDQMEKMMKPITYSLQSIWVDVFLGIILGLIMSAFIK
                     KDKNIFEQE"
     gene            5368..6318
                     /locus_tag="PLR72_04290"
     CDS             5368..6318
                     /locus_tag="PLR72_04290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK36455.1"
                     /translation="MDISVVIPLYNEEESLLELFEWIERVMNENNFSYEVIFVDDGST
                     DNSWNVIESLKRKTPYIRGIKFRKNYGKSVALHFGFQAAKGDVVITMDADLQDSPEEI
                     PALYAMIKNENYDLVSGWKKKRYDAKLTKNLPSKLYNATARKVTGIKIHDMNCGLKAY
                     RNEVVKNIELYGEMHRYIPYLAKNAGFTKIGEKVVEHNKRQYGKSKFGWNRFVNGYLD
                     LMTLWFLSKFGKKPMHFFGLIGSLMFLLGLIAVIVVGVKKLMALSAHVRAPLVTDSPY
                     FYLALLAMILGTLLFLAGFVAELIIRNSSERNNYLIEKEI"
     gene            6378..6890
                     /locus_tag="PLR72_04295"
     CDS             6378..6890
                     /locus_tag="PLR72_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36456.1"
                     /translation="MKILEIILPALLVLLTAYLLLDKLLRNEEKKRNFEIFKNNVSII
                     TPIRLRAYERLIILLERTAPNKLILNVLKPNMTNFDLQTELLNTIRQEFAHNLSQQIY
                     VSNELWNYIRTAEESLLRLVNMCASQCNPADPANVLAEKIIEVYNASEHTPTEQAIEK
                     LKAEVRSYFQ"
     gene            7150..7560
                     /gene="mce"
                     /locus_tag="PLR72_04300"
     CDS             7150..7560
                     /gene="mce"
                     /locus_tag="PLR72_04300"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOK36457.1"
                     /translation="MELKKIEHIGIAVKSLEVSIPFYENVLGLKCYAIEEVADQKVKT
                     AFFKVGETKIELLESTDPAGAIGKFIENKGEGVHHVAFLVDNVQKALDEASEKGIRLI
                     DNQPRKGAEELNIAFLHPKSTGGILIELCEQPGK"
     gene            7619..9178
                     /locus_tag="PLR72_04305"
     CDS             7619..9178
                     /locus_tag="PLR72_04305"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOK36458.1"
                     /translation="MNNQEKINYLLELRAKAKLGGGEKRIAKQHAKGKYTARERIEML
                     LDDGSFEELDMFVTHRCTNFGMEKEKYLGDGVVTGHGTIDGRTVYVYAQDFTVFGGSL
                     SETMALKICKVMDMAMKMGAPIIGINDSGGARIQEGVTSLAGYAEIFERNILASGLIP
                     QISAIFGPCAGGAVYSPALTDFILMTEQNSYMFVTGPKVIKSVTGEDISVEDLGGANV
                     HSSKSGVSHFKVETEEEGLLLIRKLLSYLPQNNLEEPPLVENFDPIDRVDDKLNTIVP
                     DNPNQPYDMKEIIRSIIDNDEFLEVHQQYARNIIVGFARMNGASVGIVANQPNFLAGV
                     LDCDASRKAARFVRFCDAFNIPLITLVDVPGFLPGSGQEYAGIITHGAKLMFAYGEAT
                     VPKVTVTIRKSYGGAHDVMSCKQLRGDFNYAWPTAEIAVMGASGAIEILEGRKIATIT
                     DPEEKEKYIAKKVEEYNDTFANPYQAASYGYIDDVIEPRDTRFRVIRALETLQTKKVV
                     NPLKKHCNIPL"
     gene            9215..9559
                     /locus_tag="PLR72_04310"
     CDS             9215..9559
                     /locus_tag="PLR72_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36459.1"
                     /translation="MKLLVINSWEELLIGAAIGIIVVFIALTILVLLFEFIQKMVISM
                     TKKKVVVKEGKKIEEIKDIPADDIAAISLALHLYFTSLHDEENHVLTIKRIERRYSPW
                     NSKIYGLTNLHR"
BASE COUNT         3320 a         1467 c         1759 g         3167 t
ORIGIN      
        1 ctatttttaa acaaaaaaat atcatgaaac ctattcaatt aattatcatt tttctttctc
       61 tgcttatccc aacattagga tacagccaga aagaaacaaa ggtgatagac gatatttatt
      121 attcgcctaa tgacaaaaca ccaacatatt cttctaaaaa tgaacaaaaa cccacttaca
      181 aaaatggtgc aaaggagatc atttttagag aacatagcga ttccatcaaa gaaaatagta
      241 ctctttcgat gactaaggag gaaaatgaca gcctcctcaa cgatacaata catgaagaag
      301 gctattattt gcatggtttt aaaggttcct cttccgacta tgaatatgct gaacgtattc
      361 gtagattcca taatccaaaa tttactattc atatttccga tcctgattac atggatttgt
      421 attttcttga tgattataac tggaatgttt atatcgaagg aggttatgcg tgggtaacac
      481 caacctggac caatcccctc tggtttgatt actattatcg accttttatg tacaatagct
      541 ggtattggcg atggagcctt tattatcctt cttattcgta ttggtatagt ccgtggtact
      601 atccatggca tgcgtggtat tatgaccctt ggtattattc cccatggtat tatggctatt
      661 atagacatcc ttattatgga ggatattatc attacaaaac gattaatcct tctcataatg
      721 aagccaaccg ccgacaaatt accaattttg gaaatgaaag aagggtagct agttcttctt
      781 ctagtgcaat gtctaccaat tcagacaatg ttagccaaag aaatggacgt tctaataatc
      841 cctatacagt tattacagga acaggaggac gaagcagtaa tacaacagga atgacaaccc
      901 caagtaatgc tggtaattca agttctgaac aaacaagaag aggtgctgtc ttaaataatc
      961 aaaatcaatc tgaaagaaca tataattctg ctaatactcg ttcttcgtca agttcttatg
     1021 cgaatagtga aaattccaga agatcaactt ctgtaaacac tcagcctcgt acttcaaata
     1081 ctacaaacaa ttcttcagga gtgagagcaa ataccagatc gacttattct gctccaagca
     1141 gaagctcatc tggtgaaagc aaccgaagtg gctccagcta ctcatcggga agttcgagcg
     1201 gaagttcaac tcgctcttca ggttcttcta atagtagtgg aggaagccag agcagtggag
     1261 gtagttcgag tgggtcaagt agaaattcaa gtggtggcgg aagacggtaa aattttataa
     1321 caattaataa attattaacc aatttattaa aacatgaaga aaaatattgg ggtatgggtg
     1381 ttattgtttt gcagtaattt gattgttgct caaagtgaat tggatatagt aaaatttgtt
     1441 caacctgata taaatggtac ggctcgatac atgagcatgg ctggagcatt tggagcgtta
     1501 gggggagatg tttcagcaat taaagataat ccagctggtt taggtattta tcaacattct
     1561 gagacagttg gaactttaaa tttcttattt caaaataacg ataatagctg gtatggagta
     1621 aattcaagtg ataatcttta taaattgaaa gcaaataatt tttcaattgt aattgcttcg
     1681 ccgacaaagc aaagtaaaaa aggaatcaaa agcggattac aaaattctaa tttttctttt
     1741 tcttttcagc aggtgaaaaa ttttaatcgc cagacaacta ttcaaagcgg agaatcacct
     1801 tcatcaataa ccgattatat ggcttatttt actgatggaa tccctgaagc agatttacaa
     1861 agtagtgaaa actaccaccc ctttaataat caaaatattc catggttatc agtattggct
     1921 tatgaaggat atttaattaa tcctacttcc aataattcat gggggtcaat attaaatgca
     1981 ggtgagaaag tcattccttc ttactatttg catgaaagtg gtcatattaa taaatattct
     2041 ttcagttggg cagggaattt cagtcatatg ttttatttgg gttttaccgg taatattcag
     2101 actattgatt ataatgctgt tactgaatat gaagaaactt ttggtgaagg tgggggtatg
     2161 aatctgaaca acactattga tgcgtcgggg agcggtttca atttagagat gggtattatt
     2221 tttcgacctg ttgacaattt cagaatcggt ctctccttcc agagccctac agttttttct
     2281 atatcagaag attatacatc tctcttaaat ttcaacactg aatcacaagg ttatatttca
     2341 acgccaactg gatataattc ctataaactt caaaccccag cagtttttga tgcaagtgcc
     2401 gcttatattt ttgggaaaaa aggacttatc agcgttgaat acaattatac cgatttaaaa
     2461 actactaagt tgatggacaa atacggttct acaaaggatt tcaaagatga gaatgcaggg
     2521 atgaaaagtg ctgtagatgc agtttctacc ataaaaattg gtggcgaatg tcgcttaact
     2581 gataatttct ctgttcgtgc tggttatgct aatatgagtg aagccacaca aaaaaatgct
     2641 gaaaaattta tgcgaatcaa tactattcga actgatacgg aatatttttt acacaatggg
     2701 acacgatacc tcacagcagg ctttggatat catgaaaatt cctggtatat tgatttagct
     2761 tatatgaata agcttacaaa agaaaaattt tatccctata actcgattag ggcaaatgag
     2821 atggttgatt gtccgatagg agaacctgct gaggtcaaaa ccattaacaa taatcttgta
     2881 ataacgttgg gattgaaatt ttaagtgtat aaataatttc cttgcttttt cattttaaag
     2941 agctgattat tttttatcgg ctctttttgt tttataaact ttagtgattt tataataaaa
     3001 tcttgttgta ttcttgaaaa tttaggaaat gcactctttg aaatcttatc aaaaaaccgt
     3061 aactttgcac accttttaca aaggtttaca ttaaaattaa ctatctgaaa atgaatttca
     3121 aagacgaaat aaatcggcga cgaacatttg ccattatcag ccatcccgat gctggaaaaa
     3181 ctactttgac agaaaaactt ttactttttg gcggtgctat tcatgtggca ggtgcagtga
     3241 aatcaaataa aatcaaaaga actgcaactt cagactggat ggaaattgag aaacaaagag
     3301 gtatttcggt agcaacttct gttatgggtt ttgaatatcg gaattataaa attaatattc
     3361 tggatacgcc tggtcatcaa gattttgcag aagatactta tcgcacactt acagcggtgg
     3421 atagtgtaat tattgtaatt gattcagcaa aaggtgtaga agaacaaaca cgaaaattaa
     3481 tcaacgtttg caggatgcga aatactccga ttatcgtgtt cattaacaaa atggaccggg
     3541 aaggaagaga tgctttcgat ttgatcgatg aacttgaaaa agaactgcaa atggaagttt
     3601 gtccgcttac ctggcctatc ggtcagggga tttcttttaa gggagtatat aatcgttatt
     3661 ataacaattt acaacttttt acgccaaaca aacaagtcgt tggttcttcc attgacattt
     3721 cagatgtttg tagtaccaaa ttagaaaaat gtgttggaga aaaagaagct cagcaacttc
     3781 gcgacgaact aaaactcata agtggtattt attcaaaatt tgatattgaa aaatatttgg
     3841 gaggtgaatt agctcctatt tttttcggta gtgctttaaa taattttggt gtaaaagaat
     3901 tattggactg ttttgtagaa atcgcccctt cacctcgccc cgtagaagct atggagcgta
     3961 aggtaaatcc agatgaagaa gtatttactg gttttgtttt taaaattcat gctaatatgg
     4021 atcctcacca tcgcagttgt attgcttttg taaaagtttg ttctggaaaa tttgagcgaa
     4081 atgtcaatta taaacatgtc agaaaaaata aaattatacg tttttcgaat cctactgcgt
     4141 ttatggctca aaagaaacaa atcgtagatg aagcttatgc gggcgacatt gtaggtattc
     4201 ctgataatgg aaattttaaa attggtgata ctttaacttc aggtgaagag ttgcatttta
     4261 aaggattgcc aagtttttca cctgaaatgt tcaagtacct tgaaaattct gacccaatga
     4321 aagctaaaca attagaaaaa ggtattcaac aattaatgga cgaaggagtt gcacaattat
     4381 ttattaacca gcataatagt cgaaaaattg tagggacagt aggtgaactt caatttgaag
     4441 taattcagta ccgattgttg catgaataca atgctcaatg ccgatgggaa actcttcccc
     4501 tttataaagc ttgctggatt gaaagtgaaa ataaagaaga actggaaaat ttcaagaaaa
     4561 gaaaagatca atttttggca aaagataagg aaggcaggga tgttttcatg gctgattcac
     4621 cttatatttt acaaatggct caaaatgact ttaaaaatat aaaattccat ttcacttcgg
     4681 agttttaaaa gctttttact tgttttttta attaatttct tattttttcc ccagaaaatc
     4741 aatttaactt attttttaat ttagtttttt gtatttttgt caaatataat ttttaaaaaa
     4801 attgttatga catccaatat ttttaaatct tccatgattt atgggttgtt tatggggatt
     4861 ttgtttgctg ccaacttttt catttcactt ccccaaggaa taatattcag attactctct
     4921 tttttgatta ttattttgat tatttatgcc atgtaccgtt tctctgttag atatcgcgac
     4981 aatgaatgtg gcgggtacat ctcatatggt aaagcatttt catttatctt gcttactttc
     5041 ttttttgctt ccatcatatc ttctattttt aaatttttct attttcaatt tattaatcct
     5101 gactatctta ctaatctttt gcaagaaagt ttaaaaataa ttgatagtat gcaattacct
     5161 gtcaatgagg aattttatga ccaaatggaa aaaatgatga aacctattac ctatagttta
     5221 caaagtattt gggtagatgt ttttttagga attattttag gattaataat gtcagctttt
     5281 atcaaaaagg acaagaatat ttttgagcaa gaataaaaat tctttataac aagaatagaa
     5341 tcaacaaggg cttataaagc aataattatg gatatttcag tcgtcattcc tttatacaac
     5401 gaagaagaat cactactcga acttttcgaa tggattgaac gagtaatgaa tgaaaataat
     5461 ttcagttatg aagtgatttt tgtggacgat ggcagcacag ataattcatg gaatgtgatt
     5521 gaatcattaa aacgaaaaac tccttatatc agaggcataa aatttaggaa aaactatggc
     5581 aaatcggtag ctttgcattt tggatttcaa gcagccaaag gcgatgttgt aattacaatg
     5641 gatgctgatt tacaggatag cccagaagaa attcctgctt tgtatgccat gataaaaaat
     5701 gaaaactatg atcttgtatc aggatggaaa aagaaaagat atgatgcaaa acttaccaaa
     5761 aatcttcctt caaaacttta caatgctact gctcgaaaag ttaccggtat taaaattcat
     5821 gatatgaatt gcggattaaa agcttatcgt aacgaagtgg tgaaaaatat agaactctat
     5881 ggcgaaatgc atcgctatat tccttatctg gctaaaaatg ctggttttac aaaaataggt
     5941 gaaaaagtgg ttgaacataa caaacgacaa tacgggaagt caaaatttgg ctggaatcgc
     6001 tttgtaaatg gttatttaga cttgatgacc ttgtggtttc tttcaaaatt cgggaaaaag
     6061 cctatgcatt tttttggctt aatcggtagt ttgatgtttt tgttgggttt gattgctgtc
     6121 attgtggtag gagttaaaaa actcatggca ctatctgcac atgtcagagc cccattggta
     6181 acggacagtc cttattttta tctcgctctt ctggctatga ttttaggaac tttacttttt
     6241 ttagccggtt ttgtagcgga actaattatc agaaattctt cagaacgaaa taattatttg
     6301 atagaaaaag aaatataaat aactttactt gttatatctt tttgtaaaaa agttaattta
     6361 ttaaaattgt tgtttcaatg aaaatacttg aaattatatt acccgcatta cttgttttgt
     6421 taactgctta tttattattg gataaattgc tccgcaacga agaaaaaaaa cgtaactttg
     6481 aaatttttaa aaataatgtt tcaattatta cccccattcg tttaagagct tacgaacggc
     6541 tcataatttt attggaaaga acagcaccca ataaattgat tttgaatgtt ctaaaaccca
     6601 atatgaccaa tttcgatctt caaacagaat tattgaacac tatcagacag gaatttgccc
     6661 acaatctttc acaacaaatt tatgttagta atgaattatg gaattacatt cggactgctg
     6721 aagaaagttt acttcgatta gtaaacatgt gtgcttcgca atgtaatcct gctgatcccg
     6781 cgaatgtttt ggcagaaaaa atcattgaag tttataatgc cagtgaacat actcctaccg
     6841 aacaagccat cgaaaaatta aaagctgaag taagaagtta ttttcaataa aaaatagttt
     6901 ttttaactat tttcttttaa atttcacttc ccaataaaag ttataggctt ttagtgtaat
     6961 tgacactcaa tcatttttaa aaaatacaaa ttcatctgtg ttttctaatg cataaatgaa
     7021 ttatatttat taattttgtg caaaaattaa atactgaaat atttctttaa ttcaactttt
     7081 tcttttatta aaggaattaa aaatttgaat attttagtaa aattatctat caacctaaat
     7141 tatatattta tggaactaaa aaaaatagaa catatcggca tagcagtaaa aagtttagag
     7201 gtatctattc ctttttatga aaatgtatta ggattaaagt gttatgcaat agaagaagta
     7261 gctgatcaga aagtaaaaac agcttttttt aaagtggggg aaacaaaaat tgaattattg
     7321 gaatcaaccg atccagcagg agcgataggg aaatttattg aaaacaaagg agaaggtgtt
     7381 catcatgttg cttttttagt ggataatgta caaaaagcac tggatgaagc ttccgaaaaa
     7441 ggtatcaggc ttatcgataa tcaaccacga aaaggagcag aagaattaaa cattgccttt
     7501 ctacatccca aatcaacagg gggaattttg attgaacttt gcgaacagcc aggcaaataa
     7561 ttttaaggat tatttttaga agttttttaa aatgggaatc aattaattat aaaatacgat
     7621 gaacaatcag gaaaaaatca attatcttct tgaacttcgt gcaaaggcaa agttgggagg
     7681 tggagaaaaa agaatagcta aacaacacgc taaaggaaaa tatacagcaa gagaacgtat
     7741 cgaaatgttg ctggatgatg gaagttttga agagctggat atgtttgtca ctcatcgttg
     7801 cacgaatttt ggaatggaaa aagaaaaata tctgggcgat ggtgtagtga ctggtcatgg
     7861 gactattgat ggtcgcactg tttatgttta cgctcaggat tttaccgtat ttggcggttc
     7921 gctttctgaa acgatggctc tcaaaatttg taaagtgatg gatatggcta tgaaaatggg
     7981 agctcctatt attggtatca atgattctgg aggtgcgcga atacaagaag gagttacttc
     8041 acttgccggt tatgcagaaa tttttgaacg aaacattttg gcttcaggtt taataccgca
     8101 aatatcagct atttttggac cttgtgctgg tggtgctgta tattcaccag cattgactga
     8161 ttttatttta atgaccgaac aaaatagtta catgtttgta actggtccca aagtcataaa
     8221 gtcagttact ggagaagata tttctgtgga agatttagga ggagcaaatg tacattcttc
     8281 aaaatcagga gtttctcatt tcaaagtaga aacagaagaa gaaggtttac tgcttattcg
     8341 aaagctttta tcctacttgc ctcaaaataa tctggaagaa ccgcctttag tagaaaattt
     8401 tgatccaatt gatcgggtag atgataaatt aaatacaatt gtacccgata atcccaatca
     8461 accttacgat atgaaagaga ttattcgatc tatcatagat aacgatgaat ttttagaagt
     8521 tcatcaacaa tatgctcgaa atattatcgt tggttttgct cggatgaatg gagcttcggt
     8581 aggaattgtt gccaatcaac ctaattttct cgcaggtgtg cttgattgcg atgcttctcg
     8641 caaagcagca cgttttgttc gtttttgtga cgcctttaat atcccattga ttactttggt
     8701 agatgtacca ggctttctac cagggtctgg tcaggaatat gcaggaataa ttactcatgg
     8761 agcaaaatta atgtttgctt atggtgaagc tactgttcca aaagttactg tcaccattcg
     8821 aaaatcgtat ggaggcgcac atgatgtaat gagttgtaag caattacgcg gagatttcaa
     8881 ttatgcctgg ccaactgccg aaattgctgt aatgggtgct tcaggagcca ttgaaatatt
     8941 ggaaggacgt aaaattgcaa caattactga tcctgaagaa aaagaaaaat atattgcaaa
     9001 aaaagtagag gaatacaatg atacttttgc aaatccttat caagctgctt cttatggcta
     9061 tattgacgat gtgattgaac cacgcgatac tcgattcaga gtcattcgcg ctttagagac
     9121 tttacaaact aaaaaagtag taaatccttt aaaaaaacat tgcaatattc ccttgtaaaa
     9181 ttagtttaca aataaataat gaaacttaat atatatgaaa ttattggtta tcaattcgtg
     9241 ggaagaactt ttgataggag ctgctattgg aattatagtg gtttttatag ctttaactat
     9301 tttagtactt ctatttgaat ttattcaaaa gatggttatt tcaatgacca agaaaaaagt
     9361 agtagtaaaa gaagggaaaa aaatagaaga aatcaaggat attccagccg atgacattgc
     9421 tgccatttct ttagctttac atctttattt cacctcatta catgacgaag aaaatcacgt
     9481 tcttaccatt aagcgtattg aacgtcgtta ttcaccatgg aattcaaaaa tttatggact
     9541 tacaaatttg catagataat gaaaaaattt gattttacta tccatggtaa tgaatatcac
     9601 gtattaataa aatccgtgga aggaaattta gttgaacttg aagttaatgg tacaccctat
     9661 acagttactc tcaatcaaga agtaaaagtt agcaaaacac ccattctggt aag
//
LOCUS       DAOSAS010000065         9511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4720_length_9511_cov_14.248096, whole genome shotgun sequence.
ACCESSION   DAOSAS010000065 DAOSAS010000000
VERSION     DAOSAS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9511
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4720_length_9511_cov_14.248096"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..400)
                     /gene="rplS"
                     /locus_tag="PLR72_04315"
     CDS             complement(53..400)
                     /gene="rplS"
                     /locus_tag="PLR72_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOK36460.1"
                     /translation="MDLLKVAEQAFQVEKSFPEFKSGDTITVAYRIKEGNKERIQEFR
                     GVVISIKGQEGKKRFTVRKMSGNVGVERIFPMDSPFIESITVNKRGKVRRAKLFYLRK
                     LTGKKARIKEKRY"
     gene            855..2669
                     /gene="typA"
                     /locus_tag="PLR72_04320"
     CDS             855..2669
                     /gene="typA"
                     /locus_tag="PLR72_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOK36461.1"
                     /translation="MQKIRNVAIIAHVDHGKTTLVDKMLLAGHLFRDNEKVGELIMDN
                     NEIERERGITILAKNASINYNGYKINIIDTPGHADFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVLQKALQLGLIPIVVINKVDKPNCRPDEVQEEVFDLMYHLNATEEQLNFH
                     TIFGSAKQNWMSEDWRKPTNNIFPLLDAIIKYIPEPFYLEGTPQMLITSLDYSPYVGR
                     IAVGRVHRGTLYEGMDVSLVKRDGFVVKTKIKELYTFIGLERKRISEVKSGDICALIG
                     IDNFEIGDSIADINQPEPLKPIAVDEPTMSMVFTINNSPFYGQEGKYITSRHLHERLM
                     RELDKDLALRVKKEPDKDIWHVYGRGVLHLSILIETMRREGYELQVGQPQVIFKMING
                     IRCEPVEQLIINLPEEYSGRIIEIIASRKGEMLKMETMNDRIQLEFIIPSRGIIGLRN
                     QVLTASGGEAIMSHRFLEYQPYKGPIEKRTNGSLIAMETGTAFAYALDKLQDRGRFFI
                     SPQEEVYAGQVVGESSKEGDIVVNVTKSKKLTNMRASGSDEKIKLTPPVIFSLEEALE
                     YIKEDEYVEITPSNIRLRKIYLDENERKRMSKNVSATA"
     gene            2729..3361
                     /locus_tag="PLR72_04325"
     CDS             2729..3361
                     /locus_tag="PLR72_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOK36462.1"
                     /translation="MKKEFWVVLFLLLIFSSKILAQFGVKIGINEANEVITSSNLSTP
                     IEKPTLQNLTAYHIGVIYQYTSKIGLGFDIGTSLSQKGCFLQSDSICDIYRELNYIEI
                     PFNIRYRLSWGIIGIYGFGGLYGAYLLKGQKITETNTSSIPEKFSYTNFNDRTDYGYT
                     FGGGIEIFKKIQLEANWNKGLKENIYYTNSDTQIFTTKNNVFSFNLTFIF"
     gene            3513..4871
                     /gene="tig"
                     /locus_tag="PLR72_04330"
     CDS             3513..4871
                     /gene="tig"
                     /locus_tag="PLR72_04330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOK36463.1"
                     /translation="MNVIRKDLDQNNAVLTLSIKKDDYAENVEKRLRELRKKSNMPGF
                     RPGMVPLQLVRKMYGKSVLVEEINQLISQKLQDYIEENKIDILGEPLPREEDQQNIDF
                     NLQEEFEFNFDLGLAPQFEISFSKEDKIKYYHIEVTDEMIDNRIKAYASRFGKYQEEE
                     TVEEKDMLKGELVQLENGKVKENGIHITDAVLTPAYMKDDGQKSLFIGAKKGDTITFN
                     PATAYQNNETELSSLLKISKEEVNNIKEDFQFKIESITRFYPAEINQELFNKVYGENI
                     VSSEEEFREKIKQEIEKELAAESNYKFQLDVRTMLIDRYKNLNYPDEFLKRWLLLKNK
                     DLTPEKLEEEYPKIIEDVTWQLIREKLLKAFNINVEPDEVENYAKEEAKNQFAQYGFI
                     NLSEDILENYAKDMINKEDTLKNLIENLFDQKIFSSIKEVVTLETNNVTLEEFDKLFE
                     TK"
     gene            4989..5678
                     /gene="clpP"
                     /locus_tag="PLR72_04335"
     CDS             4989..5678
                     /gene="clpP"
                     /locus_tag="PLR72_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOK36464.1"
                     /translation="MDKNNEFRKYATKKLGINGLLIDQYSDIVNTSYISPTILEERQL
                     NVTQMDVFSRLMMDRIIFLGTEVDDYAANVIEAQLLYLDSAEPGKDISIYLNTPGGSV
                     YAGLGIYDTMQFITSDVSTICTGMAASMGAVLLSAGAKGKRFALKHSRIMIHQPMGGA
                     QGQASDIEITAREIQKMKKELYNILAEHTGNPYEKIEKDADRDYWMTSYEALEYGMID
                     KVLINEKKTKK"
     gene            5688..6923
                     /gene="clpX"
                     /locus_tag="PLR72_04340"
     CDS             5688..6923
                     /gene="clpX"
                     /locus_tag="PLR72_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOK36465.1"
                     /translation="MPKTKKYCNFCGRSEDEVEFFILGMDGVQICNDCAMIAAEIAKE
                     NTQAKKQTLSFEKSQIPKPKEIKQYLDQYVIGQEEAKKYLSVAVYNHYKRLMQEKTDD
                     DVEIEKSNIIMVGPTGTGKTLLARTIAKKLHVPFTIVDATVFTEAGYVGEDVESILTR
                     LLQVADYDVEAAEKGIVFIDEIDKIARKSDNPSITRDVSGEGVQQGLLKLLEGTIVNV
                     PPQGGRKHPEQKMIPVNTQNILFICGGAFEGIERKIAARLNTRVVGYNSIEGTKQIDR
                     NNLIKYIMPQDLKSFGLIPEIVGRLPVLTYLNPLDRDALRQILTEPKNSIVKQYIKLF
                     KMDNIELTFDEEVLDYIVDKAIEFKLGARGLRSITETIMMDAMYEMPSKKAAKKLTIT
                     LDYAQNKLQQTNLDQLKIA"
     gene            7154..9337
                     /gene="recQ"
                     /locus_tag="PLR72_04345"
     CDS             7154..9337
                     /gene="recQ"
                     /locus_tag="PLR72_04345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOK36466.1"
                     /translation="MAKKTALAVHLKKYFGFDTFKGNQEEIIQNVLDGNDTFVLMPTG
                     GGKSLCYQLPSLIMDGTAIVISPLIALMKNQVDAMRNFSEEDGVAHFLNSSLTRQAID
                     QVKADVLSGKTKLLYVAPESLTKEENTEFLKDVKISFYAIDEAHCISEWGHDFRPEYR
                     RIRPIINEIGVAPIIALTATATPKVQHDILKNLGILEAKIFKSSFNRHNLYYEVRQKT
                     DNIDKEIIKYIRSQEGKSGIIYCLSRRKVEELAEMLQVNGIAALPYHAGMDSEQRTIN
                     QDKFLMEEANVIVATIAFGMGIDKPDVRYVIHYDIPKSLEGYYQETGRSGRDGGEGQC
                     IAFYSRKDLDKLRKFMQRKPIAEQEIGYQLLLETQAYAESSVCRRKLLLHYFGENYIE
                     ENCGNCDNCLKPRKFMEGQELLTLILETISQVKEKFKEEHIVDILKGNETTDVLSYQH
                     NELENFGAAMDEDEQLLHAVIRQAMIAGYITKDIENYGLLKLTPQGKAFLKNPTSFPI
                     VKDNEFEEEEEIGTLNAASTCAADDALFSILKDLRKKLSKKLEVPPFALFQDPSLEAM
                     ATSYPITLEELQNIPGVGTGKAKRYGEEFLKVIKEYVKENEVVRPDDLRVRTVANKSK
                     LKVSIIQAIDRKIALDDIAISKGLDFNDLLNEIEAIVYSGTKINIDYFLDEVMDPERV
                     DEIFEYFRTAETDDIDVALEELGEDDFSETEIRLVRIKFLSEVGN"
BASE COUNT         3414 a         1348 c         1698 g         3051 t
ORIGIN      
        1 ttttaaacaa aaaagagaga aaattgactt tctctcttcc aaatttcttt ctttaataac
       61 gtttttcttt tatccttgct tttttacctg ttaacttacg taaataaaac agttttgcac
      121 gacgtacttt acctctttta tttacagtaa tgctttctat aaaaggcgaa tccatcggaa
      181 aaattctttc cacaccgaca tttcccgaca ttttgcggac agtaaaacgt tttttccctt
      241 cctgtccttt aatgctgata actacaccac gaaattcctg aattctttct ttgttccctt
      301 ctttaattcg ataggcaact gttatggtat caccactttt aaattcaggg aaactttttt
      361 ctacctgaaa agcctgttct gcaaccttta ataagtccat ttttattaat tttatttatt
      421 acagcacaat atttacatac ttttcgtttc tgccagagat tgtgcttaaa ttggaacgca
      481 aaaatactat ttttttttat tgcatacaat aatttcatcc ttttttgaat taaaaacttt
      541 tttaattgtt aactatcaat gatttaagtg taaaaaagct tatctttgtt gttgatgaat
      601 tgaattgatg ctttttaaaa tttattatga gttgttgatt tttaaaaggt tgataagatt
      661 tcaatcattt ttttattctt acatttttcc ctattccttt gtttttatta tcctaaaagg
      721 aaatttcaag taattttttc ctttttttgt tgtccaataa tagatgatta ttcaagtaat
      781 ttgaataata tagccaatta aaattcaatt tttttgatta aatttgcaag cttaaaaaat
      841 gaggagttgg atacatgcaa aaaattagaa atgttgcaat tattgctcat gttgatcacg
      901 gaaaaactac actggttgat aagatgttgt tggctggtca tcttttcaga gacaatgaaa
      961 aggtcggcga attaatcatg gacaataatg aaatagaacg tgaaagagga attacaattc
     1021 tggcaaaaaa tgcttcgatc aattataatg gctataaaat caatattatt gatacgccag
     1081 gtcatgccga ctttggtggt gaagtagagc gggtactcaa catggctgac ggagttttac
     1141 tgttggtaga tgcttttgaa ggaccaatgc ctcaaacacg atttgtttta caaaaagctt
     1201 tgcaattggg acttattcct attgttgtga ttaacaaggt ggataagcct aattgtcgac
     1261 cggatgaagt tcaggaagaa gtttttgatt taatgtatca tttgaatgcc acagaagaac
     1321 aactgaattt ccatacaatt tttggttctg ccaaacaaaa ttggatgtca gaagattgga
     1381 gaaaaccaac aaacaatatt ttcccgctat tggatgctat tataaaatat attcctgagc
     1441 ctttctatct ggaaggaaca cctcaaatgc tgattacttc tttggactat tctccttatg
     1501 taggtagaat tgcagtggga cgtgttcatc gtggtacgct gtacgaaggt atggatgtaa
     1561 gtttagtgaa gcgagatggt tttgtggtga aaactaaaat taaagaacta tacactttta
     1621 tcggactgga acgaaaaaga atttcagaag taaaatcagg agatatttgt gctttaatcg
     1681 ggattgataa ttttgaaatt ggtgattcca ttgcagatat taatcagcct gagcctttaa
     1741 aacccattgc tgttgatgaa ccaaccatga gtatggtgtt taccattaac aattctcctt
     1801 tttatgggca ggaaggaaaa tacatcactt caaggcactt acatgaacga ttgatgcgtg
     1861 aattggataa agatcttgct ttgcgagtaa aaaaggaacc tgataaagat atttggcatg
     1921 tttatggcag aggtgtgctt catttatcta ttttaataga aaccatgcgc cgcgaaggtt
     1981 atgaattgca agtgggacaa ccgcaagtaa ttttcaagat gataaatggc atccgttgtg
     2041 aacctgttga acaactgata attaatctcc ctgaagaata tagtggcaga attattgaaa
     2101 ttatagcttc tcgtaaggga gaaatgttga aaatggaaac gatgaacgat cgtattcaat
     2161 tagaatttat aattccttca cgaggcatta ttggacttcg caatcaagtg ctaactgcat
     2221 caggcggtga agctattatg agtcatcgtt ttcttgaata tcaaccttat aaaggaccta
     2281 tcgaaaaaag aacaaatggt tcattaattg caatggaaac aggcacagct tttgcatacg
     2341 cattggacaa attacaagat cgaggtagat tttttatttc accccaagaa gaagtttatg
     2401 cgggacaggt ggtaggagaa agctcaaaag agggtgatat tgtagtaaat gtcactaaat
     2461 ctaaaaaatt aaccaatatg agagcttctg gctcagatga aaaaatcaaa cttactcctc
     2521 ctgtcatatt tagtctcgaa gaagcgttgg aatacataaa agaggatgaa tatgtggaaa
     2581 ttactccttc taatattcga ttacgaaaaa tctatcttga tgagaatgaa cgaaaacgaa
     2641 tgtcaaaaaa tgtttctgct actgcataat taaattcttt tatttctcat tgatatttaa
     2701 aacaaggaag tacaaaaatt tatttttcat gaaaaaagaa ttttgggttg tattatttct
     2761 tttactgata tttagttcaa aaattttagc tcaatttggc gtgaaaatcg ggatcaatga
     2821 agcaaatgaa gttattactt caagtaattt atctactccc atcgaaaagc ctactttgca
     2881 gaacttaact gcttatcaca taggagtaat ttatcaatat acttcaaaaa ttggcttagg
     2941 ctttgatatt ggcacatctc tctctcaaaa aggttgtttc cttcaatcag acagcatttg
     3001 tgatatctat agagaattaa actatattga aatacctttc aatattcgtt atcgtttatc
     3061 atggggaatc atcggaattt atggctttgg aggtttatat ggagcctatc ttttaaaggg
     3121 acaaaaaatt actgaaacaa atacttcttc tattccagaa aaattttctt ataccaattt
     3181 caatgatcga accgattatg gctatacctt tggtggcggg attgaaattt ttaaaaaaat
     3241 acaattggaa gcgaactgga ataaaggctt gaaagaaaat atttattata caaattcaga
     3301 cacacaaatt tttactacaa aaaataatgt cttctctttt aaccttacat ttatattcta
     3361 attatgaata atttattaat tatttgatta tcaaataatt aaatttattc atagagtttt
     3421 ttaaacataa aatgttttct atcagttctt ttttcgtacc tttgtcttca ttaaaaaact
     3481 aaatatcaga aaattaatta aaaaaataag ttatgaatgt cattagaaaa gaccttgatc
     3541 aaaacaacgc tgtacttact ttaagcatca aaaaagacga ttatgcagaa aatgtagaaa
     3601 aaagattgcg ggagttacgt aaaaaatcaa atatgccagg atttcgtcca ggaatggtac
     3661 ctttgcagtt ggtaagaaaa atgtatggga aatcagtcct tgtagaagaa attaaccaac
     3721 tcatatctca aaagctgcaa gattatatcg aagaaaacaa aattgatatt ctgggcgaac
     3781 ctcttccacg agaagaagat cagcaaaata tagattttaa cttacaggaa gaatttgaat
     3841 ttaattttga tttaggattg gctcctcaat ttgaaatatc attttccaaa gaagataaga
     3901 ttaaatatta ccacatcgag gttaccgatg aaatgattga caatagaatc aaagcgtatg
     3961 cttctcgttt tggaaaatat caggaagaag aaaccgttga agaaaaagac atgttgaaag
     4021 gagaattagt gcaattggaa aatggtaaag tcaaagaaaa tggaattcat attactgatg
     4081 cagtacttac accagcttat atgaaggatg acggtcaaaa atcattgttt atcggtgcaa
     4141 aaaaagggga cacgattact tttaatccag ctactgctta tcaaaacaac gaaacagaat
     4201 tatcttcctt attaaaaatt tcaaaagaag aagtaaacaa tattaaagaa gattttcaat
     4261 ttaaaattga gagtattact cgtttttatc ctgctgaaat caatcaagaa ttattcaata
     4321 aggtttatgg tgaaaatata gtgtcaagcg aagaggagtt tcgtgaaaaa ataaaacaag
     4381 aaattgagaa agaacttgct gctgaaagta attataaatt tcaattggat gttcgtacga
     4441 tgttgataga tcgatataaa aacttgaact atcctgatga atttttaaaa cgctggttat
     4501 tgctcaaaaa taaagaccta acgccagaaa aattggaaga agagtatcca aaaatcattg
     4561 aagatgttac ctggcaattg attagagaaa aattgctgaa agcttttaat ataaatgtgg
     4621 aacccgatga agtagaaaat tatgccaaag aagaagcaaa aaaccagttt gcgcaatacg
     4681 gatttattaa tcttagcgaa gacatccttg aaaattatgc aaaggatatg ataaataaag
     4741 aggatacttt aaaaaactta attgagaatt tgtttgatca aaagattttt tcttccataa
     4801 aggaagttgt aactttagag acgaataatg ttaccttaga agaatttgat aaattgtttg
     4861 aaaccaaata attttaaaag acaatttgaa tttagaattg aaactgaaac ttttttaaaa
     4921 taattttgtt tataaaaagt tctctaccaa tgagaacgat aaatttttta taaaaacaaa
     4981 taactattat ggataaaaat aatgaatttc gcaaatatgc aaccaaaaag ttgggaataa
     5041 atggcttgtt aattgaccaa tacagcgata ttgtaaatac aagttatatt tctcctacca
     5101 ttcttgaaga acgtcaactg aatgtcactc aaatggatgt gttttcaaga ttaatgatgg
     5161 atcgaattat ttttctgggt accgaagtag atgattatgc tgccaacgta attgaagctc
     5221 aattacttta tcttgattca gcagaaccag ggaaggatat ttctatttat ctgaatacac
     5281 caggaggttc agtttatgct ggtctgggta tttatgatac tatgcagttc attacttctg
     5341 atgtttctac tatctgtact ggcatggcag cttcgatggg agcggtacta ttatctgctg
     5401 gtgcaaaagg gaaacgtttt gctcttaaac attcacgtat catgattcat cagccgatgg
     5461 gtggagctca agggcaagcc tcagacattg aaatcacggc tcgtgaaatc cagaaaatga
     5521 aaaaagaact ctacaatatt ctggcagaac atacgggcaa tccttacgaa aaaattgaaa
     5581 aagatgctga tcgagattat tggatgactt cttacgaagc cctggaatat ggaatgattg
     5641 ataaagttct catcaacgaa aagaaaacaa aaaaataatg cttaaaaatg cctaaaacca
     5701 aaaaatattg taatttttgt ggtcgttcag aggatgaagt agaatttttt attttaggaa
     5761 tggatggcgt tcagatttgc aacgattgtg ctatgattgc cgcagaaatt gcgaaagaaa
     5821 acacccaagc caaaaaacaa actctttctt ttgaaaagtc gcaaataccc aagccaaaag
     5881 aaataaaaca atatctggat caatatgtta tcgggcaaga ggaggcaaaa aaatatttat
     5941 cagtagccgt ttataatcat tataaacgtt taatgcagga aaaaaccgat gatgatgtag
     6001 aaatagaaaa atccaacatt atcatggtag gacctacagg tactggtaaa accttgctgg
     6061 caagaacaat agcaaaaaaa ctacatgtac cttttactat tgttgatgca acggtattta
     6121 ctgaagcagg ttatgtgggg gaggatgtag aaagtattct tacacgactt cttcaggtag
     6181 cagattatga tgtagaagct gctgaaaaag gtattgtttt cattgatgaa atcgataaaa
     6241 tagctcgaaa aagtgataat ccaagcatta cccgcgatgt aagtggtgaa ggagtgcaac
     6301 aaggtttgct gaaattgttg gaaggaacca ttgtcaatgt accaccacaa ggaggtagaa
     6361 aacatccaga acaaaaaatg attcctgtaa atacacagaa tattttgttt atttgtggcg
     6421 gagcctttga aggcatcgaa agaaaaattg cagctcgtct taataccaga gtggtcggat
     6481 ataattcaat agaaggcact aagcaaattg atcggaataa tcttataaaa tacattatgc
     6541 ctcaagatct caaatctttt ggactaattc cggaaattgt aggccgtctt cctgttttga
     6601 cttatcttaa tcctttagat agagatgctt tgcgtcaaat tcttactgaa cctaaaaatt
     6661 ctattgtcaa gcagtatatt aaactgttta aaatggacaa tatagaatta acttttgatg
     6721 aagaagtttt agattacatt gtggataaag ccattgaatt caagttgggt gccagaggat
     6781 tgcgttccat tacagaaaca attatgatgg atgccatgta tgaaatgcca tcaaaaaaag
     6841 cagcaaaaaa gctaactatt acgttagact acgctcagaa taaattgcaa caaaccaact
     6901 tggatcaatt aaaaatagcc tgaaataata tttctaaaat tttcattaaa aagcaggata
     6961 ttaattattt ttattaatgt cctctttttt ttaaaaaatg cttgtatatt tgaaaagtgt
     7021 ttataatttt gtttgcctgt aaatgtgaaa aaataaatca agaaaggaaa taaaagattt
     7081 ttcattaaag tagaaaaatt catactaatt tttaaatact aattttactt attttgtgat
     7141 cgaggattgt tgtatggcta agaaaactgc tttagcagtg catttaaaaa aatattttgg
     7201 ttttgatact ttcaaaggta atcaggaaga aattattcaa aatgtattgg atggcaatga
     7261 tacttttgtt ttaatgccaa caggaggagg taaatcttta tgctatcagt tgccttcttt
     7321 aataatggat ggcacggcga tagttatttc acccttaatt gctttaatga aaaatcaggt
     7381 agatgccatg cgtaatttca gtgaagaaga tggtgtggca catttcctta attcatcatt
     7441 aacacggcaa gcaattgatc aagttaaagc tgatgtttta tcaggtaaga cgaaattgct
     7501 ctatgtggct cctgaatcac ttacgaaaga agaaaatact gaatttctaa aagatgtaaa
     7561 aatttctttt tatgccattg atgaggcaca ttgtatttca gaatgggggc atgatttccg
     7621 tcctgaatat cgccgaatca gacccattat caatgaaata ggagttgctc ccatcatagc
     7681 tctaacagct actgcaactc caaaggttca acatgacatt cttaaaaatt taggaatatt
     7741 agaagctaag atatttaaat cttctttcaa tcgtcataat ttatattatg aagttcggca
     7801 aaaaactgac aatattgaca aagaaattat taaatatatc cgttcacagg aaggaaaatc
     7861 aggtattatt tattgtttga gtagacgaaa agtcgaagaa ttggccgaaa tgttgcaagt
     7921 aaatggtata gccgctttac cgtaccatgc aggaatggat tcagaacaac gcacaatcaa
     7981 tcaggataaa tttttaatgg aagaagccaa tgtaattgta gcaaccattg catttggtat
     8041 gggaattgat aaaccggatg tacgatatgt tattcattat gatattccta aaagtttaga
     8101 aggctattat caggaaacgg gtcgaagtgg ccgcgatgga ggagaaggac aatgtatagc
     8161 tttttattca agaaaagatt tagataaatt acgaaaattt atgcaaagga aaccaattgc
     8221 tgaacaagaa attggttatc aacttttgct tgaaacgcaa gcttatgctg aatcttccgt
     8281 ttgtagaaga aaattattac ttcattattt tggagaaaat tatatagaag aaaattgtgg
     8341 aaattgtgat aactgtttga aaccacgaaa atttatggaa gggcaagaat tattaacttt
     8401 aatattagaa actatttctc aagtaaaaga aaagtttaaa gaagaacaca ttgtagatat
     8461 tcttaaagga aatgaaacta cagatgttct ttcttatcag cacaatgagc tggaaaattt
     8521 tggagcagcg atggacgagg atgaacaatt attgcatgct gttattcggc aagcaatgat
     8581 tgcaggatat attacaaaag atattgaaaa ttatggatta ttgaaattga ctccacaagg
     8641 gaaagctttc ttaaaaaatc ctacttcttt tcctattgtg aaagataatg aatttgagga
     8701 ggaagaagaa ataggtactt taaatgctgc cagtacttgt gcagcagatg atgcattgtt
     8761 ttctattttg aaagatttac gcaaaaaact ctctaaaaaa cttgaagtgc cgcctttcgc
     8821 attatttcaa gatccttcgt tagaagctat ggctacttct tatcctatta cattggaaga
     8881 attacaaaat atccctggtg taggaactgg caaagcaaaa cgctacggag aagaattttt
     8941 aaaagtgatt aaagaatatg taaaagaaaa cgaagtagtt cgtccagatg atttgcgtgt
     9001 aagaacggta gctaataaat caaaactaaa agtatctatt attcaggcaa ttgaccgaaa
     9061 aattgcactt gatgatattg ctatatcgaa aggtctggat ttcaatgatt tactcaatga
     9121 aatcgaagct atcgtatatt ctggtacaaa aattaatatc gactattttt tagatgaagt
     9181 aatggatccg gaacgtgtag acgaaatttt tgaatatttc agaacggctg aaactgatga
     9241 tattgatgtt gcattagaag aattaggcga ggatgatttc agtgaaacag aaattcgatt
     9301 ggtgagaatt aaattcttat cggaagtagg aaattaaaaa tagtttttcg actttttaaa
     9361 aaatctcgca ccttaaggta attttctttt ttgaaacgga ttttcctttc aaaggaggta
     9421 tattttatgt acaacaaatt gattattatt tgtttaatat atttttttaa aataatttct
     9481 tgcttacagt gtaattatta gttgtttatc a
//
LOCUS       DAOSAS010000066         9502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4731_length_9502_cov_12.903885, whole genome shotgun sequence.
ACCESSION   DAOSAS010000066 DAOSAS010000000
VERSION     DAOSAS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9502
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4731_length_9502_cov_12.903885"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..985
                     /gene="panC"
                     /locus_tag="PLR72_04350"
     CDS             140..985
                     /gene="panC"
                     /locus_tag="PLR72_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOK36467.1"
                     /translation="MKVVSTKNELQSEIEKIKGKKKSIGLVPTMGALHKGHLSLIEHC
                     INENDISVVSIFVNPTQFNNKKDLEKYPRNVAKDIQLLDDLGCTLVFTPSVEEMYEDK
                     ELNQPFEFDFGGLDTMMEGKFRPGHFNGVVQIVSKLFQLVQPDRAYFGEKDFQQLAII
                     RYMVKKMKFDIDIVPCPIVREANGLAMSSRNELLTPEQRKIAPEISKILFASRELVPE
                     LSPSAIENWVTEKLNNIPAFKVDYFEIVNAETLLPVQSWKESAIGCVAVYCGEVRLID
                     NVRYN"
     gene            1031..1381
                     /locus_tag="PLR72_04355"
     CDS             1031..1381
                     /locus_tag="PLR72_04355"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOK36468.1"
                     /translation="MLVSILKSKIHRVTVTEANLNYKGSITLDETLMEAANLLPNEKV
                     IVVNNDNGERIETYVIPGKRGSGIVCLNGAAARKALPGDIVIIMSFALMEIEEAKLFK
                     PTIIYPDTATNQLI"
     gene            1390..1977
                     /gene="hisH"
                     /locus_tag="PLR72_04360"
     CDS             1390..1977
                     /gene="hisH"
                     /locus_tag="PLR72_04360"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOK36469.1"
                     /translation="MDVAVIKYNAGNIRSVLFALERLQVNTIVTDEEDKIRSADKVIF
                     PGVGEASSAMAYLKDKKLDKVILSLTQPVLGICLGMQLMCSFSEENDTPCLGIFKETV
                     KHFPKNSYKVPQIGWNNIYDLSNTLFSGIAENSYVYFIHGFYVGVSKYTIAKTNYILE
                     YSAALHKNNFYAVQFHPEKSGKIGQKILENFLFQV"
     gene            2008..3138
                     /gene="tgt"
                     /locus_tag="PLR72_04365"
     CDS             2008..3138
                     /gene="tgt"
                     /locus_tag="PLR72_04365"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOK36470.1"
                     /translation="MFFEVLHNDVDSNARIGKITTAHGVIETPIFMPVGTAGSVRAVH
                     FRELKEDIKAQIFLGNTYHLYLRPGINILEEAGGLHKFTGWDLPILTDSGGYQVFSLS
                     EIRKLHEEGVMFRSHIDGSKHLFTPENTVDIQRSIGADIIMALDECTPGTADYQYAKK
                     SMELTHQWLKRGIKRFDETQPKYNYFQAFFPIVQGCIYPDLRKESAEVIAAENRDGNA
                     IGGLSVGEPTEKMYEMIEIVNEILPKNKPRYLMGVGTPQNILESIQRGIDMFDCVMPT
                     RNGRNGMLFTLQGIINIKNAKWKNDFSPLDEFGTSYVDKVYSKAYLHHLFINKEILAL
                     QIASIHNLAFYLWLMAEARRHIQNNDFTQWKKSIIQQLGERI"
     gene            3187..4287
                     /locus_tag="PLR72_04370"
     CDS             3187..4287
                     /locus_tag="PLR72_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOK36471.1"
                     /translation="MKVDYQKIGLKRIDTYIINKFLGTFFFCMVLILSISVIFDLTEK
                     LDNFFESNAPLKAIVFDYYLNFIPFYLNMFTPLFTFIAVIFFTSKMAGDNEIIAILSS
                     GVSFNRLMLPYFLSATFIALFSFGLGGYVIPHSTKKMLDFENQYVKKFKPENARNVQM
                     EIEKGVILYIERFEITTNRGYHFSLEKFDGKKLVSRLTAESIKWDSAYHWTITNYLQR
                     DFVGMHEKIKKGDVLDTVINVQPKEFFITSKESAQLSNNELRNYLHRQRERGVGNIQA
                     FENEYHKRYSMPFAAFVMTLIGVSLSSRKVRGGMGLHLGIGIALSALYILFSTISTTF
                     SVSGVMSPFLAVWLPNFVFILIGFYLYSTAPK"
     gene            complement(4360..5229)
                     /gene="speE"
                     /locus_tag="PLR72_04375"
     CDS             complement(4360..5229)
                     /gene="speE"
                     /locus_tag="PLR72_04375"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOK36472.1"
                     /translation="MMLEKNYPTVTFNEYDEINEYGVTIGINKIIYSEQTKYQKIDIF
                     ETKNFGKIFTLDGIVMTRDSDEFIYHEMISHVPLFLHDNPKNVLVIGGGDGGTVREVL
                     KHSSVEKVTMCELDEKVVEASKKYLPNLSYELTNPKVKLIFDDGSNFIKKFKNKFDVI
                     IIDSTDPTEEGGGILYTEEFYRNCSEALTENGVFSAQTEEPFLEKVWMNKAYKNISSV
                     FNIARLYMGFVPAYFPGTWTWTFASKNIDPIKDFKAEKVTNFKEKLKYYNEEIHIASF
                     ALPNFVKEIINVI"
     gene            complement(5234..5632)
                     /gene="speD"
                     /locus_tag="PLR72_04380"
     CDS             complement(5234..5632)
                     /gene="speD"
                     /locus_tag="PLR72_04380"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOK36473.1"
                     /translation="MTKSLGKHLIAELYDCDEKILSNVSEIENQMSKAAIECGATIVA
                     STFHHFSPHGVSGMIIISESHLAIHTWPEYKYVSLDIYTCGNSVDPWIAFHNLKDAFK
                     SKRQEAVEYKRGIFKTLGIPEDSSHKVELK"
     gene            complement(6389..9061)
                     /locus_tag="PLR72_04385"
     CDS             complement(6389..9061)
                     /locus_tag="PLR72_04385"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HOK36474.1"
                     /translation="MNHFYNQNIDEVLRQFDVTAEKGLSAEEAKKRFQQYGPNQLETI
                     KQKTTWQIFISQFRGSMVMILFVAAIISGVIGLRNGEGLIDTIVILAILIVNAIIGTV
                     EEKKAQSSLEALKKMSAPHSKVLRDGEIIEIPTIEIVPGDIVVLDTGDIIPADMRLIE
                     AVNLKIQESSLTGESVPVEKNTETLNTENVPLGDRKNMAFSTCVVTFGRGKGVVVATG
                     MKTEVGNIAHMLQHTEETETPMGKRLEQLGKILGYVAIIICAVIFIVGLLYGNDWLDM
                     FMMAVSLAVAAIPEGLQIVSTLVLSIGVQRMVKLNAIVRRLPSVESLGSVTVICSDKT
                     GTLTQNKMTVVKSWTIENEIDFRNQNISEKWNNTQQILLQSALLCTDAHLRILPDGTH
                     ENAGDPTETAIVDIALNLGINKNEEEKKFPRIAEVPFDSERKRMATINKMENGKLRVN
                     VKGGIDEVLSVTSYLLVQDKVRPITDEDITIIRKQNENMAGKALRILAVAYKDIDKLP
                     DKVTVETIENNLIFVGLLGMIDPPRPEAKEAVAKCKVAGIHPVMITGDHKITALAIAK
                     EIGIYQEGDETVTGSDLEKMSDDELYRHVKGYSVYARVAPTHKVRIVKAWQSYGDIVA
                     MTGDGVNDAPALKQADVGVAMGIVGTEVTKDVSDVILTDDNFATIVNAVEEGRRIYDN
                     ILKTIQYLLSSNVGEVLLILIASLLNLGNPLTPILILWINLVTDSLPALALSMDPPEK
                     DIMTRKPRNPKKGFFSKGMVWRIIYQGITLGLISLAAYLIGFKDGGQPLGQTMAFAVL
                     GFAELIHVRNLHSNKISCFKTSLFANKALIGAIVLSAAFMVLILLIPPLMNIFGIVAM
                     DKIHWIYVLLLSLTPIAVVELMKLFKINSSKDEY"
BASE COUNT         3145 a         1553 c         1559 g         3245 t
ORIGIN      
        1 gaacttcgga gaggaagaaa ttcgatttgt ttacaaaaaa gaaaataagc aaagaaattt
       61 tagtaatttt gcattgtttt tcaaaacagg aaaatttgac tgagcaaaaa ctgtctaatg
      121 aatacatttt gattcaataa tgaaagtagt ttcaactaaa aatgaacttc agtcggagat
      181 tgaaaaaata aaaggaaaaa agaaaagtat aggtttggtt ccaacgatgg gagctttaca
      241 taaaggacat ttatcgttga ttgaacattg tatcaatgaa aatgatattt ctgtggtgag
      301 catttttgtc aatcccactc agtttaacaa taaaaaagat ctggaaaaat atccacgtaa
      361 tgtcgcaaaa gatattcaat tacttgatga tttaggttgc actctggttt ttacgccttc
      421 tgttgaagaa atgtatgagg ataaagaact gaatcagcct ttcgaatttg attttggtgg
      481 acttgataca atgatggaag gtaaatttag accaggacat ttcaatggcg tagtacaaat
      541 agtgagcaaa ttatttcagt tagtgcaacc tgacagagct tattttggag aaaaagactt
      601 tcagcaatta gctattattc gttatatggt aaaaaaaatg aaattcgata ttgacattgt
      661 tccttgcccg attgttagag aagcaaatgg tttagccatg agcagtcgga atgaactttt
      721 aactccagaa caacgtaaaa ttgctcctga aatttccaaa atattatttg caagccgtga
      781 acttgtccct gaactttcac cttcagctat tgaaaattgg gtaaccgaga aattaaataa
      841 cattccagct tttaaggttg attatttcga aattgtcaat gctgaaacct tgcttcctgt
      901 tcaatcatgg aaagaatcgg ctataggatg tgttgcagta tattgtggcg aagtaaggtt
      961 aattgataat gttagatata attgattcct ttcttttgaa aaaattaatc caaaaaatta
     1021 atccaaaatt atgttagtga gtattttaaa atctaaaatt catagagtca cagtaacaga
     1081 agctaatttg aattataaag gcagtattac tcttgatgaa actctaatgg aagcagccaa
     1141 tctacttcct aacgaaaaag tcatagtagt caataatgat aatggagaaa gaattgaaac
     1201 gtatgttata cctggcaaac gaggttcggg tattgtttgc ttgaacggag ctgcagctcg
     1261 taaggcttta ccaggagaca tagttatcat tatgtctttt gctcttatgg aaattgaaga
     1321 agctaaactg ttcaaaccta ctattatata tcctgacacg gctactaacc agctcattta
     1381 actttttata tggatgttgc cgtaataaag tataatgcag gaaatattcg ttccgtgctt
     1441 ttcgctttgg aacggctaca ggtgaataca attgttactg atgaagaaga taaaatccgc
     1501 agtgctgata aagtgatttt tccaggcgtg ggagaggctt cttcggcaat ggcatatctt
     1561 aaagacaaaa aactggataa agtgattctt tcactgactc aacccgtttt aggtatttgt
     1621 ttgggaatgc aattaatgtg ttctttttca gaagaaaatg acaccccttg tttgggaatt
     1681 tttaaagaaa cggtaaaaca ttttcctaaa aatagttata aagttccaca gattggctgg
     1741 aataatattt acgatttgtc gaataccctt ttttcaggaa ttgcagaaaa ttcctatgtc
     1801 tattttatac atggttttta tgtaggagta tcaaaatata ccattgcaaa aacgaattat
     1861 attttagaat atagtgctgc tttacataaa aataattttt atgcagtaca atttcatcca
     1921 gaaaaatctg gtaaaattgg tcaaaaaatt ttggagaatt ttttatttca agtttaaaaa
     1981 ttatgtgttt ggttaacttg tgaacaaatg ttttttgaag ttttacataa cgatgttgac
     2041 agtaatgccc gtattggaaa aattaccact gcacatggcg tcatcgagac gccaattttt
     2101 atgccagtag ggacagcagg ttctgttaga gctgtacatt tcagagaatt aaaagaagat
     2161 attaaggcac aaattttctt aggaaatact tatcacctgt atttgcgtcc gggaataaat
     2221 attttagaag aagctggtgg attacataaa tttacgggat gggatttgcc catattaacc
     2281 gatagcggag gttatcaagt tttttcgctt tccgaaattc gtaagttgca tgaagagggt
     2341 gtgatgtttc gttctcatat tgatggcagc aaacatcttt tcacacctga aaacactgtt
     2401 gatattcaac gttctattgg agcagatatt ataatggcac ttgatgaatg tacccctggc
     2461 actgctgatt atcaatatgc aaaaaagtca atggaattaa ctcatcagtg gctaaaacgg
     2521 ggaataaaac gttttgatga aactcaaccc aaatataatt attttcaagc ttttttcccc
     2581 attgttcaag gatgtatcta tcctgactta cgaaaagaat cggctgaggt tattgctgct
     2641 gaaaatagag atgggaatgc tattggagga ttatcagtgg gtgaaccaac agaaaaaatg
     2701 tatgaaatga ttgaaattgt aaatgagatt cttccaaaaa ataaacctcg ttacttaatg
     2761 ggagttggaa cgcctcaaaa tattctggaa agtattcagc gaggcattga tatgttcgac
     2821 tgtgtgatgc ccacacgaaa tggtcgaaat ggaatgcttt ttaccttaca aggaattata
     2881 aatattaaaa atgctaaatg gaaaaatgat ttttctcctc tcgatgaatt tggaacatct
     2941 tatgtagata aggtttactc aaaagcttat ttacatcact tatttatcaa taaagaaata
     3001 ttagctttgc agattgcttc aattcacaac ttggcttttt atttatggct gatggctgaa
     3061 gctcgtcgtc atattcagaa taatgatttt acacaatgga aaaaaagcat cattcaacaa
     3121 ttgggtgaaa gaatataaaa atattttgga gaaacatcca ctaaaagaaa attttcaata
     3181 aagcgaatga aagttgatta tcagaaaatt gggcttaaac gaattgacac ctatattatt
     3241 aataagtttt taggtacttt tttcttctgt atggttttga tattaagcat ttctgttatt
     3301 tttgatttaa cagaaaaact ggataatttt tttgaaagca atgctccatt aaaagcaatt
     3361 gtttttgatt attacttgaa ttttattcct ttctatctga atatgtttac tcctctgttt
     3421 acttttattg ccgtcatttt tttcacttca aaaatggctg gcgacaatga aattattgct
     3481 attctttcca gtggagtaag ttttaataga ttgatgctac cctattttct ttcggctact
     3541 tttattgctc ttttttcttt tggattagga ggttatgtta tccctcattc caccaaaaaa
     3601 atgcttgact ttgaaaatca atatgttaaa aaattcaagc ctgaaaatgc tcgaaatgtt
     3661 cagatggaaa ttgaaaaagg cgtgatttta tatattgaaa gatttgaaat tactaccaat
     3721 agaggttatc atttttcgct ggaaaagttt gatgggaaaa aactggtttc cagacttact
     3781 gctgaatcga tcaagtggga ctcagcttac cattggacaa ttaccaatta cttacaaaga
     3841 gattttgtcg gaatgcatga aaagattaaa aaaggagatg ttctggatac agtaataaat
     3901 gtccaaccta aggaattttt tattacctcc aaagaaagtg ctcaactgag taacaatgaa
     3961 cttcggaatt atttgcatcg gcagcgtgaa cggggtgttg gtaatattca agcatttgaa
     4021 aatgaatatc acaaacgtta ttctatgcca tttgctgcat ttgtaatgac tttaatcggt
     4081 gtttccttat cttcaagaaa agtacgaggt ggaatggggc ttcatcttgg catcggaata
     4141 gcactgagtg ctttatatat tttattttca accatttcaa ctactttttc agtaagtgga
     4201 gttatgtcac catttttagc agtgtggcta ccaaattttg tttttatttt aattggtttt
     4261 tatttgtatt ctacagcacc gaagtaattt tataagaaag aaataatacc aatttgcttt
     4321 caaaaataga aaaatatttt tttgatttct gaccagaact caaataacat ttattatttc
     4381 tttaacgaaa tttggtaaag caaatgatgc tatgtgaatt tcttcattat agtattttaa
     4441 tttttccttg aaatttgtaa ctttttcagc tttaaaatct tttatgggat ctatattttt
     4501 tgaagcaaat gtccaagtcc atgttccagg aaagtatgct ggcacaaatc ccatgtataa
     4561 tcttgcaata ttaaatactg aactgatatt tttgtaagct ttattcatcc atactttttc
     4621 taaaaatggt tcctctgttt gagccgagaa aacaccattt tcagtaagtg cttcagaaca
     4681 atttctataa aattcttccg tgtaaagtat tcctccacct tcttctgtcg gatccgttga
     4741 atcaataatt attacatcaa atttattttt gaactttttg ataaagttac ttccatcatc
     4801 aaatatcaat ttaacttttg gattggttaa ttcgtacgat aaattaggta aatatttttt
     4861 agaggcttct acaacttttt catctaactc acacattgtg actttttcta ctgacgaatg
     4921 tttcagtact tctctaacag ttccaccatc tccgcctcca attacaagta catttttagg
     4981 gttatcatga aggaataaag gaacatgaga aatcatttca tgataaataa actcatctga
     5041 atctctggtc ataacaattc catcaagagt aaatattttc ccaaagtttt ttgtttcaaa
     5101 aatatctatt ttttgatatt tagtttgttc agaatagatg attttattga taccaatggt
     5161 aacaccatac tcattaattt catcgtattc gttaaaagtt acagtaggat aatttttttc
     5221 taacatcatt gttttatttt aattcaactt tgtgagatga atcttctggt attccaagag
     5281 ttttaaatat tcctcttttg tattcaacag cttcttgtct tttggactta aaagcatctt
     5341 ttagattatg aaaagctatc cagggatcaa ctgaatttcc gcacgtataa atatctaaag
     5401 aaacatattt gtattcaggc catgtatgaa ttgccaaatg agattctgat attattatca
     5461 taccacttac accatgaggt gaaaaatgat gaaaggtgga tgctactatg gtagctccac
     5521 attcaatagc tgcttttgac atttgattct caatttctga aacgttgcta agtatttttt
     5581 cgtcacaatc gtataattca gctattaaat gtttgcctaa agattttgtc attccttttc
     5641 cctcctttaa aatttttata aataaagatt tcactttaaa gtataaaaaa ttataacgtg
     5701 atttatatcg ctaaaagttt aaaaattaaa aaaatagatt taatcacgtt attttataaa
     5761 tgcaaatata attgaatttt tttaatttga gaggtgaatc tataatatga catcatttgt
     5821 tactaatttg tttttctatt aaccttttcc attatttttt gatttagttt tttgggggat
     5881 atattgcatc cctaacttcg caaaatttac gtaataggta gttccaattt tttcaaaagg
     5941 ctgacagctt tcttggtaat ttcagcaata tatcatttgt tgttgatttt ccttgtttta
     6001 tttcagttaa ggaataaatc aaaaatcttt aggtgaaaat tccccgttca gttcatgctt
     6061 tgtcagcaac accatatttg ataataccaa gataaagcat tagttatcgt ttattttccc
     6121 aaatataaac gaaacgcctt tacgtctttg atattgacct gatttattct gtgtaataag
     6181 ttgttattct actgaaaaaa acaaatatta aattaataat cgatccatct cgtttgcttt
     6241 tccgattcaa taaatccatc cttttcagta attctatcaa gaagttttcc tgttacttaa
     6301 gaaaaatccg ttagaaagtt ctaatctaat aacaaatttg ggtgaaaaca attccagatt
     6361 cttattgata ataaattacc ttgaaagttt aatattcgtc ttttgaacta ttaatcttaa
     6421 aaagtttcat caattcaacc acagcaatag gagtcagtga aagcaacaat acatatatcc
     6481 aatgaatttt gtccatagca acaatgccaa aaatattcat tagtggcgga attaaaagaa
     6541 tcaacaccat aaaagctgca gaaagaacaa tagctcctat caaggcttta ttggcaaata
     6601 aactggtttt gaaacaagat attttattag aatgaagatt tcggacatga attaattcag
     6661 caaatccgag tacagcaaaa gccatagttt gtcccagtgg ttgaccgcca tctttgaatc
     6721 cgatgaggta agcagccagt gaaattaaac caagggttat tccctgataa attatacgcc
     6781 aaaccattcc tttggaaaaa aatccctttt tagggtttcg tggtttacgt gtcatgatat
     6841 ctttttctgg tggatccata cttaacgcca gagcgggcaa actatctgtt acaagattta
     6901 tccataaaat aagtattgga gtcagtggat tgccaagatt cagcaatgaa gctataagaa
     6961 ttaataaaac ctctcccaca ttggatgata atagatattg aatagttttg agaatattat
     7021 cgtaaatccg gcgaccttct tccacagcat taacaatagt agcaaaatta tcgtcggtaa
     7081 ggataacatc cgatacatct tttgttactt ctgtgcctac tattcccatt gcaactccta
     7141 catcagcttg cttcagtgca ggagcatcgt taacaccgtc accagtcata gcaacaatat
     7201 ccccatagga ttgccaagcc tttacaatac gaactttgtg agtaggtgcc actcgtgcat
     7261 atacagaata tcctttgaca tgacgatata attcatcatc agacattttt tcaagatcag
     7321 agcctgtaac ggtttcatcg ccttcctgat aaatgccaat ttctttagca atggcaagag
     7381 ctgtgatttt atgatcgcct gtaatcatta ctggatggat accagccact ttacattttg
     7441 ctactgcttc tttcgcttca ggacgtggag gatcaatcat tccaagtaat cctacaaaaa
     7501 taagattgtt ctcaatggtt tcaacggtca ccttgtcggg aagtttatca atatccttat
     7561 aggctactgc aagaatacgt aaagcctttc ctgccatatt ctcattctgt tttctgataa
     7621 tagtaatatc ttcatcagta attggacgaa ctttatcttg caccaaaaga taagaagtga
     7681 cacttaatac ttcatctatt cctcctttca cgttaacacg tagtttcccg ttttccattt
     7741 tgttgatagt agccatacgc ttacgttccg aatcgaatgg aacttcagcg atgcgtggaa
     7801 attttttttc ttcttcattt ttatttattc ccagattcaa ggctatatcc acaattgctg
     7861 tttcagttgg atcacctgca ttttcgtgag tgccatcggg aagaattctt agatgagcat
     7921 cagtacaaag caatgccgat tgtaacagaa tttgttgggt gttgttccat ttttcagaaa
     7981 tattttgatt acgaaaatca atttcatttt ctatagtcca actttttacc actgtcattt
     8041 tattctgagt aagagtgcca gttttatcgg aacaaatcac ggtaacgcta cccaatgact
     8101 ctaccgaagg cagtctacgt acaatagcat taagttttac cattcgttgt accccaatag
     8161 aaagcaccaa agtagaaaca atctgaagcc cttcgggaat ggcagcaact gcaaggctaa
     8221 cagccatcat aaacatatcc agccaatcgt tcccgtaaag taaacctaca ataaaaataa
     8281 cagcacaaat aataattgca acataaccta aaattttgcc aagctgttct aaccttttcc
     8341 ccatcggcgt ttcagtttct tcggtatgtt gaagcatatg ggcaatattt cccacttcag
     8401 ttttcatgcc agtagctact acaacacctt tgcctcgtcc gaaagttact acacaggtag
     8461 aaaatgccat attttttcga tcacctaaag gaacattttc tgtattcaaa gtttcggtat
     8521 ttttctctac gggtaccgat tctcctgtca aggaagattc ctgaatttta agattgactg
     8581 cttctatcag tcgcatatca gcaggaataa tatcacctgt atccagcact acaatatccc
     8641 caggaactat ttcaatggta gggatttcaa taatttctcc atcacgtaaa accttagaat
     8701 gtggagcact cattttttta agagcctcta aagaggattg agcttttttc tcttctactg
     8761 ttccaataat ggcattaaca ataaggatag caagtataac aatggtatca attaatccct
     8821 ctccatttcg taacccaatc acacctgaaa taatagcagc cacaaaaaga atcatcacca
     8881 ttgatcctct gaattgactg ataaatattt gccaggtagt tttttgtttt atggtttcta
     8941 attgattggg accatattgc tgaaaacgtt tttttgcttc ttcagcagat aaaccctttt
     9001 cagcggttac atcaaattgc cgaagtactt cgtctatgtt ttgattataa aaatgattca
     9061 ttgaggtaga atataactgt ttaaaaacaa ataaaagatg agtttaactc ataaaaatct
     9121 acaaaatcat ataaaaaata gtttttaaac agttcctgga tcaacgccag ggagaaaaaa
     9181 ttgcttgata aaacagaaat ttatcttagt aattttttga aagaatggat ttaatttgca
     9241 aaataatttt ttcatcattc caactgaaaa ggctaaaaat ggaaaaaggt agattttaag
     9301 taattaatac aattttaaga ggaaataaaa agtaaaaaaa atttttcaag acaccggaaa
     9361 aaagtgtttt tccccttgta ctgatgaaat aaatgtttta ctttggtctc tttttctcta
     9421 aaaagttaca aagaaaaaat acttaactgg gacgcaaaga tacacaattt gtgtaatata
     9481 acaacggtga tgcaagcaaa aa
//
LOCUS       DAOSAS010000067         9471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4748_length_9471_cov_13.510089, whole genome shotgun sequence.
ACCESSION   DAOSAS010000067 DAOSAS010000000
VERSION     DAOSAS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4748_length_9471_cov_13.510089"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..2056
                     /gene="dnaG"
                     /locus_tag="PLR72_04390"
     CDS             107..2056
                     /gene="dnaG"
                     /locus_tag="PLR72_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOK36475.1"
                     /translation="MIDQATIDRIIQAAPIQEVISEYVTLKRRGANLIGLCPFHNEKT
                     PSFTVSPSKGIFKCFGCNKGGNVVHFIMEHEQMTYYEALKFLAKKYNIEVQEKELTPE
                     EIEAHNERESLLLVNDFANKYFMNILHHHNEGRTVGLSYFQERGFREDIIQKFQLGYC
                     LDSRDAFTQAALKEGYKKEFLIKTGLTIESENNYFLDRFRGRVIFPIHSLSGKIIAFG
                     GRVLKKEEKAAKYLNSPESEIYHKSNELYGLYFAKQAIVRENRCYLVEGYADVISFFQ
                     SGIENVVASSGTSLTPGQIHLIHRFTENVTVIYDGDAAGIKASLRGIDLLLEEGLNIK
                     VVLLPEGEDPDSFAHKNNASDVIAFIQQNSVDFIRFKTNLLLSDAGNDPFKRANLIMD
                     IVQSIAIIPNPIVRAEYVKECSNLLSVEEQMLYHEINKLIKKQREKNIKSTSLISGDS
                     TTKISSSQPPIDVNEQKFDEEERNILQVLIKYGKWVLYYEDIENTLPVTVEDYILKEL
                     EQDQLHFNNALYALVLDEYHKNSQQENFNAENYFLHHTNSEISKLVANLISQKYTLSK
                     IYSKVKKIDAESEKLRDYVERVILEYKNSIVVSQLREKLIEMKKATEDKNEELVKQLM
                     KEIEQLEEIKNQLSKNLGERIIIKI"
     gene            2078..2713
                     /locus_tag="PLR72_04395"
     CDS             2078..2713
                     /locus_tag="PLR72_04395"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HOK36476.1"
                     /translation="MTYQELIQQPDYVQLASVPHSEIKSFIKNEISEMPKWARIANMY
                     QLIGLLAFMLGLFKAFMPFFVYREVNYLIWLGLGTVFTFTFLIILHEFIHAIAYWCIG
                     SRHLSFGFQLKKFLFYVECDREVVNYQQFKKIALAPTIAVTILSLLGMILFYQQQLFY
                     FFLPIFSLHSLFAAGDFGLLCFFQNRPDWEIVTFDVKEEQKTYFYGKKKQN"
     gene            2804..3574
                     /locus_tag="PLR72_04400"
     CDS             2804..3574
                     /locus_tag="PLR72_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK36477.1"
                     /translation="MILFDKIVFGPVHSRRLGISLGINLLPIDAKICTYDCLYCECGF
                     NTRMKKSPLPKREEVSAALEAELQKMISLEKIPDIITFAGNGEPTIHPDFENIIEDTL
                     QLRDIYCPLAKVGVLSNATEIHKPQVFRALNKVDDNILKFDTAFDHTLQLLNRPTQKY
                     ITVKWLIEHLKKFEGKMIIQTLFIRGEYNGEKVDNTTDEEINAWLQALREMNATQIMI
                     YTIDRETPLKNLEKIPLNELNTIAEKARNYGFDVSIST"
     gene            3645..4589
                     /locus_tag="PLR72_04405"
     CDS             3645..4589
                     /locus_tag="PLR72_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36478.1"
                     /translation="MKILVCPLNWGLGHATRCIPIIQKLLAEKNEVIIAADGSPLQLL
                     RQEFPSLRWIEFPSYKVQYSSKNSQIATILKWLPFILYGIVKEHYWLKKIIAQEHFDQ
                     VISDNRFGLWNRKIHSIYITHQLMIKMPRNNKMLEWIAWKLHRFIIQNYNECWIPDTE
                     SAPGLSGDLAHKYPLPNNAKFIGPISRFQNLPYIPSQKKFEVVVIVSGLEPQRTLFEQ
                     KMIQLHKKNPYQTLIIQGQPQQKITTQKIGNITLISHLPAIEMASLLKNADKIFARSG
                     YSTIMDLFTLNCLSKAVFSPTPGQTEQIYLSEIHSTNT"
     gene            4727..5860
                     /gene="nspC"
                     /locus_tag="PLR72_04410"
     CDS             4727..5860
                     /gene="nspC"
                     /locus_tag="PLR72_04410"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOK36479.1"
                     /translation="MIDYSQVPSPCFVLDEVAFRKNLALIKSVKDRSGAEIILAFKAF
                     AMWSVFPIVREYISGAAASSLWEARLAFEEMKTKAHTYSPAYTEKEFPEILNYSSHIT
                     FNSLSQFYKFHSQTKEKNISCGLRINPEFSPVTTNLYNPCLPGSRLGILAQQLGEKLP
                     EGIEGLHFHVLCESDAEDSAKLLQVIENKFGKYFSQLKWLNLGGGHLMTKKDYNIELL
                     VSVLKSFKVKYPHIQLILEPGSAFAWQAGVLVATVTDIVENKGIKTALLNVSFSCHMP
                     DCLEMPYKPTIIGATDEIPGKPTYRMGGNSCLAGDFCGSWSFDKELKVGDRIVFDDMI
                     HYTIVKTTMFNGVTHPSISLWTKDNQFELIREFTYEDYKNKLS"
     gene            5892..6266
                     /locus_tag="PLR72_04415"
     CDS             5892..6266
                     /locus_tag="PLR72_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HOK36480.1"
                     /translation="MKIIGNILWLILGGLVIAAEYLISSLLLMVTIVGIPFGIQTLKL
                     SVLALWPFGSDIQSTSAETNFLFVLMNIIWLLVGGIWIALTHIVFGILCAITIIGIPF
                     AKQHFKLAALALTPFGKEISTI"
     gene            complement(6347..9364)
                     /gene="secDF"
                     /locus_tag="PLR72_04420"
     CDS             complement(6347..9364)
                     /gene="secDF"
                     /locus_tag="PLR72_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOK36481.1"
                     /translation="MQNKGLVKVFFIALSLVCLYYLSFTIVSSHYSRKAKQYAGGDPL
                     KEYAYLDSLATKKVWLGYTLKECREREINLGLDLKGGMNVVLEISVPDIIRALSNYNT
                     TPNFVKALEIATERQKNSQADFLDLFVQAYKELDPHAKLASIFSTYQLKDKISLNSTN
                     EEVITVLRQEIDDAISNSFNVLRTRIDRFGVVQPNIQKLSTTGRILIELPGIKEPERV
                     RKLLQGSANLEFWETYDLNEIFNNLMEANRILASIQVVDNQATESKDTVQTKITPTQE
                     KQSEVDSLKAILAKKAEITSDSLKMLEQRKKENPLFSVIQLNTINGQIGRGPLVGLVH
                     SKDTALVNYYFSLPQVKEVLPRDLSFRWSVKPRDEKGEWFELIAIKVTNRDGRAPLGG
                     NVITDARVDFGQTSAYANVSMSMNAEGSKIWARLTKDNIGKSIAIVLDGYVYSYPTVQ
                     NEIKGGQSQITGNFTIEEAKDLANVLKSGKMPAPARIVQEDVVGPSLGQEAIQSGFIS
                     FIIAFVMVLIYMIFFYGWIPGLVADIALFANVFFLLGILASFSAVLTLPGIAGIVLTL
                     GMAVDANVITYERIREEMRAGKNMKRAVQEGYKNALSAIIDGQATTLITGIILAIFGT
                     GPIKGFATTLIIGIITSFITAVFLSRLIIEWYVSRDNAKEISFTTNLTKNFLQNTHIN
                     FIGIRKYAYTFSGICIFIALISFAVRGFDMGIDFTGGRNYIVRFDQPVQTDHVRSLLE
                     NSFEEAGVQVITIGSENQVRISTKYKIDDNSENVENEIEQRLYTGLKPLLKENVTQEQ
                     FVQDYIKSSQKVGPTVASDIRNAAIWSIVFALLAIGLYILIRFHELGYALGAVLSLLH
                     DAIITIGLFSLFYSVMPFSMEINLSFIAAILTVLGYSINDTVIIYDRIREYIKLYPKR
                     DRLSIMNNAINATLSRTFNTAMTTLLVLIIIFIFGGETIRGFIFALLFGIGIGTYSSV
                     FVASPLTYEYYKWKTKREAKKIGGTKKVIVKTT"
BASE COUNT         3191 a         1595 c         1536 g         3149 t
ORIGIN      
        1 ataatttgta gaataaattc attagctttg taataataaa cctatagagc aatactttaa
       61 ctaatcattt ctcttccttt gttttaattt agtttttttg caagacatga ttgatcaagc
      121 taccattgat agaattattc aggctgcacc tattcaagaa gttatttcgg aatatgttac
      181 cttaaaacga cgaggagcca atttaattgg attgtgtcct tttcataacg aaaaaacacc
      241 ctcctttaca gtttcgccca gcaagggaat ttttaaatgt ttcggatgta acaaaggtgg
      301 caacgtggtt cattttatta tggaacacga acaaatgacg tattatgaag ctcttaaatt
      361 tctggcaaaa aaatacaaca tagaagttca ggaaaaagaa cttactcctg aggaaataga
      421 agctcataat gaacgtgaaa gtttattgct ggtcaacgat tttgccaata aatatttcat
      481 gaacattctg catcatcaca acgaggggag gactgtcggt cttagttatt ttcaggaacg
      541 cggttttcgc gaagatatta ttcaaaaatt tcaattgggt tattgtctcg attctcgcga
      601 tgcttttact caagctgctt taaaagaagg ttataagaaa gaattcctca tcaaaacggg
      661 tttgacgata gaatcagaaa ataattattt tttagatcgt tttcgaggaa gagtaatttt
      721 tcccattcat tcattatcgg gtaaaataat tgcttttgga ggaagagttt taaaaaagga
      781 ggaaaaagct gccaaatacc ttaattctcc tgaatcggaa atttatcata aaagtaatga
      841 actgtatggt ctctattttg caaaacaagc cattgtccgt gaaaacaggt gttatttggt
      901 agaaggttat gcagatgtaa tttcattttt tcaaagcgga attgaaaatg tagtagcttc
      961 gtcgggtact tcgctgacgc ctgggcaaat tcatcttatc caccgcttca cagaaaatgt
     1021 aacagtaatt tatgatggcg atgctgctgg tatcaaagct tcattaagag gaatagacct
     1081 tcttttggaa gaaggcttga acattaaagt agtgttgctt cccgaaggag aagaccccga
     1141 ctcatttgcc cataaaaaca atgcttctga tgtaatagca tttatccaac aaaactcagt
     1201 tgactttatt cgctttaaaa ctaacttatt attgagcgat gctggtaatg acccttttaa
     1261 acgtgccaat ttgattatgg atattgtgca aagcattgct attatcccaa atccgatcgt
     1321 aagagctgaa tatgtgaagg aatgcagcaa cttgttgtcg gttgaagaac agatgcttta
     1381 ccatgaaatc aataaattga ttaaaaagca aagagaaaaa aacataaaat ctaccagtct
     1441 catttccggc gattcaacta ctaaaatttc gtcttctcaa ccaccaattg atgtaaatga
     1501 gcaaaaattt gatgaagaag aacgcaatat tcttcaagtc ttaatcaaat atgggaaatg
     1561 ggtattgtat tatgaagaca tagaaaatac attacctgtt acggttgaag attatatttt
     1621 aaaagaatta gaacaagatc aacttcactt taacaatgct ctttatgctc ttgtgctgga
     1681 tgaatatcat aaaaatagtc aacaggaaaa ctttaatgct gaaaattatt ttcttcatca
     1741 taccaatagt gaaattagta aattagtagc caatttgatt tcccagaaat atacgcttag
     1801 caaaatttat tcaaaagtca aaaaaattga tgctgaatca gaaaaattaa gggactatgt
     1861 ggaacgtgtg attttagaat ataaaaacag cattgttgta tctcaattgc gggaaaaatt
     1921 gatagaaatg aaaaaagcaa cggaagacaa aaacgaagaa cttgttaaac aattaatgaa
     1981 agaaattgag caactggaag aaataaaaaa tcaattgtca aaaaatttgg gtgaacgcat
     2041 tattattaaa atttaactat tattacgctg aaataaaatg acttaccagg aattaatcca
     2101 acaacccgat tacgttcaac ttgcctctgt tcctcatagt gaaattaaaa gttttataaa
     2161 aaatgaaata agcgaaatgc ctaagtgggc acgcattgca aatatgtatc agctaattgg
     2221 tttacttgct tttatgcttg gtttgttcaa agcttttatg cctttttttg tttatcgtga
     2281 agttaattat ttaatctggc ttggtttggg aactgttttt acctttactt tcctgatcat
     2341 tttgcatgaa tttattcacg ccattgctta ttggtgcatt ggcagccgtc atttgtcctt
     2401 tggttttcaa ctaaaaaaat ttttgtttta cgtcgaatgc gatagagaag ttgttaatta
     2461 tcaacaattt aagaagatag ctctggctcc tactattgct gttaccattt tatcactgtt
     2521 aggaatgatt ttattttatc aacaacaact tttttatttt tttcttccta ttttctcgct
     2581 ccatagtctt tttgcagcag gagattttgg cttgttatgt ttttttcaaa atcgacctga
     2641 ttgggaaatt gtaactttcg atgttaaaga agaacaaaaa acttattttt atggcaagaa
     2701 aaaacaaaat taatattaaa aattaagcag tcgctgataa accaattctt tctattattt
     2761 gtttgttatc atctgttttt ttaacaaata ttcaattagt aaaatgatct tatttgataa
     2821 aatagttttt ggtcccgtac atagcagaag gttaggtatt tcgctgggaa ttaatttatt
     2881 acctattgat gcaaaaattt gtacttacga ttgtctctat tgtgaatgtg gcttcaatac
     2941 aagaatgaaa aaatcccctc ttccaaaaag agaagaggtg tcggctgcac ttgaagctga
     3001 attgcaaaaa atgatttcac ttgagaaaat tcctgatatc ataacttttg caggaaatgg
     3061 tgaacctacc attcatcctg actttgaaaa cataattgaa gatactttgc aacttcgtga
     3121 tatttattgt ccccttgcaa aagtgggagt tttatctaac gcaactgaaa ttcataaacc
     3181 acaagttttc agagctttaa ataaagtaga tgataacatt ttgaaatttg atacagcctt
     3241 cgaccatact ttgcaattgc tgaaccgacc cacacagaaa tatattacag ttaaatggct
     3301 gattgaacat ttaaaaaaat ttgaagggaa aatgattatt caaactcttt ttataagagg
     3361 tgaatataac ggagaaaaag tggataatac tacagatgaa gagattaatg cctggttaca
     3421 agctcttcgt gaaatgaatg caactcagat aatgatttat actatcgata gagaaacacc
     3481 tctaaaaaat cttgaaaaaa ttcctttaaa tgaattaaat actattgccg aaaaagctcg
     3541 taattatggt tttgatgttt ctatttccac ataattttta aaaaaacaag ctggtttatt
     3601 attgctttaa taaaaaaacg gaaaatatac aactccagat tgttatgaaa attttggttt
     3661 gtcctttaaa ttggggatta ggtcatgcta cccgatgcat accgattatt caaaaattgc
     3721 ttgctgaaaa aaatgaggtg atcattgcag cagatggttc tccacttcaa ctgttacgac
     3781 aagaatttcc ttctcttcga tggatagaat ttccctccta caaggttcaa tacagttcta
     3841 aaaattctca aattgcaact atcctcaaat ggctaccttt tattttatat ggaattgtca
     3901 aagaacatta ctggttaaag aaaataatag ctcaagaaca ttttgatcag gtgatttctg
     3961 ataatcgttt tggtttatgg aatcggaaaa ttcattccat ttatataact catcagctca
     4021 tgataaaaat gcctcgaaac aacaaaatgt tagaatggat agcgtggaaa ttgcatcgtt
     4081 tcattattca aaattacaac gaatgctgga ttcctgatac tgaaagtgct ccaggcttat
     4141 caggcgatct ggctcataaa tatcctcttc ctaataatgc aaaatttatt ggtccaattt
     4201 cccgttttca aaatttacca tatattcctt ctcaaaaaaa atttgaagta gttgtaattg
     4261 tttcaggatt ggaaccgcaa agaactttat ttgaacaaaa aatgattcaa cttcacaaaa
     4321 aaaatcctta tcaaacattg ataattcaag gacaaccaca gcaaaaaatt acgacccaga
     4381 aaattggcaa tattaccctt atttctcatc ttcctgctat tgaaatggca agtctgttga
     4441 aaaatgctga taaaattttt gctcgttctg gttattccac tattatggat ttatttacac
     4501 tgaattgttt gtcaaaagca gttttttccc ctacaccagg acaaacagaa caaatatatc
     4561 tatctgaaat tcattccact aacacataaa ttgttttttc tttattttca aaattttttt
     4621 gaactaaaaa aattgttact acttttgtag aaaacgccaa tgtttttatt tttatagctt
     4681 aatttctaat caaactcttt ttcattattc ataaatatat ctttcaatga tagattattc
     4741 acaggttcct tctccttgtt ttgtgttgga tgaagttgca ttcagaaaaa atctggcatt
     4801 gataaagtct gtaaaagatc gtagtggagc tgaaattatt ttggcattca aagcatttgc
     4861 catgtggagt gtatttccca ttgttcgaga atatatttcg ggagcggcag ccagctcatt
     4921 gtgggaagcc cgtcttgctt ttgaagaaat gaaaacgaaa gcccatactt attcgccagc
     4981 ttatacagaa aaagaatttc ctgaaatttt aaattatagc agtcatatta cttttaattc
     5041 attatcacaa ttttacaagt ttcattctca gacaaaagaa aaaaatattt cctgcggttt
     5101 acgcattaac cctgagtttt cacctgtaac cacaaattta tataaccctt gtttaccggg
     5161 ttcaaggttg ggaatacttg ctcaacaatt gggagaaaaa ttaccagaag gaattgaagg
     5221 tttgcatttt catgttttat gcgaatctga tgcagaagat tcggcaaaat tgcttcaagt
     5281 gatagaaaat aaatttggta aatatttttc acaactgaaa tggttgaatt tgggcggcgg
     5341 tcatctcatg acaaaaaaag attataatat agagcttctt gtttctgttc tgaaaagttt
     5401 caaggtaaaa tatcctcaca ttcagcttat tctcgaacca ggaagtgcat ttgcctggca
     5461 agcaggtgtt ttagtagcca ccgttacaga tattgtagaa aataaaggca tcaaaacagc
     5521 tctcttgaat gtttcatttt cttgtcacat gcccgattgt ctggaaatgc cgtataaacc
     5581 gacaatcata ggtgcaacag acgaaattcc aggtaaacca acttatcgca tgggaggaaa
     5641 cagttgtttg gcaggtgatt tctgcggttc gtggtcgttt gataaagaat taaaagttgg
     5701 tgaccgcatt gttttcgacg atatgattca ctatacaata gtaaaaacga cgatgttcaa
     5761 tggagtaaca catcccagta taagtttatg gacaaaagac aatcaattcg aacttatcag
     5821 agaatttact tacgaagatt ataagaataa attatcttga tttagaatta cttataaata
     5881 atcaaaatac aatgaaaata attggaaaca ttctgtggtt aattttggga ggattggtta
     5941 tcgctgctga atatctcatt tccagcttat tattgatggt aaccatcgtt ggcattcctt
     6001 ttggaattca aacattaaaa ttatccgttt tagctttgtg gccctttgga agcgatattc
     6061 aatcaacctc tgctgaaacc aattttttat ttgtcctgat gaatataatc tggttactgg
     6121 ttggtggcat ttggattgct ttaacgcata tcgtttttgg tattttatgt gccattacta
     6181 ttattggcat tccctttgca aaacagcatt ttaaactcgc ggcattagca cttacaccct
     6241 ttgggaaaga aatttctaca atttaaaata ctgatgtaaa tttctatcta tttttcatta
     6301 aaaaaagccc catcaagatt ttctcaatag ggctttttag catttattaa gtcgttttga
     6361 caattacctt ttttgttcca ccgatttttt ttgcttctct ttttgttttc catttataat
     6421 attcgtatgt aagaggagaa gcaacaaaaa cagaagaata agttcctatt ccaataccaa
     6481 ataataaagc aaagataaat ccacgaatgg tttcaccacc aaaaatgaat ataatgatca
     6541 ataccaataa tgtagtcata gcagtattaa aagttctgct caatgttgca ttgatggcat
     6601 tgttcattat gcttaatcta tctcgtttag gatataattt gatgtattct ctgattcggt
     6661 cataaataat tacggtatca tttatagaat atcctaaaac tgtcaagatt gccgctatga
     6721 atgataagtt aatttccatc gagaagggca taacactgta gaataatgaa aatagtccta
     6781 ttgtgataat agcatcatga agcagggaca acacagctcc taatgcatat cctaattcat
     6841 ggaaacgtat taaaatatac aaacctatcg ctaataaagc gaaaacaatc gaccaaatgg
     6901 cagcgtttct gatatcactc gctaccgtag gaccaacctt ttgagaactt ttaatataat
     6961 cttgaacaaa ttgttcttga gttacatttt cttttaacaa aggttttaaa ccagtgtaca
     7021 atcgttgttc aatctcattt tcaacatttt cagagttatc atcaattttg tatttagtag
     7081 aaatgcgcac ttgattttct gaaccaattg tgatgacttg tacgccagct tcctcaaaag
     7141 aattttccaa taaagaacgc acatggtcgg tttgcacagg ctgatcgaac cgcacaatgt
     7201 aatttcgtcc gcctgtaaaa tcaataccca tatcgaaacc tctaactgca aaagaaatta
     7261 aagcaataaa tatacagata cctgagaaag tataggcata tttccgtatc ccaataaaat
     7321 taatatgtgt attctggaga aagtttttag taaggttggt agtaaatgaa atttccttag
     7381 cattatcgcg acttacgtac cattcaatta ttaaacgaga caaaaacact gctgttatga
     7441 aagaagtaat aataccaata attaaagttg tggcgaagcc tttaataggt ccagttccga
     7501 aaattgcaag aataatacct gtaattaaag ttgttgcttg cccatcaata attgctgaaa
     7561 gtgcattttt gtaaccttcc tgtacggctc gtttcatgtt tttccctgct cgcatttctt
     7621 ctctgatacg ttcataggta attacattgg catcaaccgc catacccaat gttaacacaa
     7681 tacctgctat accaggtaat gtcaataccg ccgaaaagga agctaaaatt cccagaagga
     7741 aaaagacatt ggcaaacaag gcaatatcgg ctactaaacc gggaatccaa ccataaaaga
     7801 atatcatgta aatcaatacc atcacaaaag cgatgataaa tgaaatgaaa ccgctctgaa
     7861 tagcttcttg tcctaatgat ggtcccacta catcttcttg aactatacga gcgggtgcag
     7921 gcattttacc tgactttaat acatttgcta aatcttttgc ttcttcaatg gtaaaattac
     7981 ctgtaatttg agactgacct cctttaattt cattttgtac agtagggtaa gagtatacat
     8041 aaccatccag cacaatggct attgatttac caatattatc ttttgtcaat cgagcccaaa
     8101 ttttggaacc ttcagcattc attgacatgc taacattggc ataagctgat gtttgaccga
     8161 aatcaactcg agcatcggta ataacattac ctcctaaagg agcccgtcca tctcgattag
     8221 taacttttat agcaattaat tcaaaccatt cgcctttttc atcgcgaggt ttgacactcc
     8281 aacgaaaact caaatcacgc ggcaatactt ctttaacctg aggaagacta aaataataat
     8341 tcaccaaagc tgtatctttg ctatgaacca atccaacaag gggacctcgt ccgatctgac
     8401 cattgatagt gttgagttgt attacagaaa ataaaggatt ttcttttttt ctttgttcca
     8461 acattttaag tgaatcagaa gttatttcag ctttttttgc caaaatagct ttcaaactat
     8521 ctacttcaga ttgtttttcc tgtgtcggag taatctttgt ttgaaccgta tctttgcttt
     8581 ctgttgcttg attatcaaca acttgaatgc ttgctaaaat acgattagct tccattaaat
     8641 tattaaaaat ctcatttaaa tcataagttt cccaaaattc caaattagct gaaccttgta
     8701 aaagtttgcg aacacgttcg ggttctttta tccctggcaa ttctattaaa attcttcccg
     8761 tggttgacag tttttgaata ttgggttgaa ccaccccaaa acgatcgata cgagttctta
     8821 aaacattaaa tgaattgcta atggcatcat caatttcctg tcttaatact gtaattactt
     8881 cctcattggt agaatttaag gagattttat ctttcaattg ataggtgcta aaaatagaag
     8941 ccagtttagc atgagggtcg agttctttat atgcttgtac gaataagtcc agaaaatctg
     9001 cttgactgtt tttctgtcgt tcagtagcta tttctaaggc ttttacaaaa tttggagtcg
     9061 tattgtaatt agataaagca cgtataatat ccgggacaga tatttcaagt accacattca
     9121 tgccaccctt taaatcaagt cctaaattta tttccctttc tcgacattcc tttaaagtat
     9181 aacccaacca aacttttttt gtagctaaag aatcaagata agcatattct tttagggggt
     9241 caccacctgc atattgtttc gcttttcttg aatagtgact acttacaata gtaaaggata
     9301 aataatacaa acatactaac gacaaggcga taaaaaaaac cttaacaagt cccttatttt
     9361 gcatttgaca attattttaa gtttaaaagt aagtatagaa tacaaaattt aacgttgcaa
     9421 atatactata ttttgaagta gtaaaaaaat aacatttaaa ataatttagt t
//
LOCUS       DAOSAS010000068         9413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4790_length_9413_cov_13.091045, whole genome shotgun sequence.
ACCESSION   DAOSAS010000068 DAOSAS010000000
VERSION     DAOSAS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9413
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4790_length_9413_cov_13.091045"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..2197)
                     /locus_tag="PLR72_04425"
     CDS             complement(209..2197)
                     /locus_tag="PLR72_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK36482.1"
                     /translation="MFPNISKVKANPVIDTTKIYLIPEIVVTGRIKPPEVRSSTPLQI
                     LTSEQIKNLNVLQVSDAVKYFSGISVKDYGGIGGLKTVSVRGLDANHTAVSYDGITLS
                     DCQTGQIDLGRFSIENVDLLTFNNGQNDNIFQPARLFASASVLNIQTSAPYFKEKEKF
                     NGKISFKSGSFGLVNPFFFFQGKINQQFSAVVSSEWLSANGKYPYLLKYGTGVNDSTS
                     WEKRKNTDVKNFRLEGALYYKENENSSGYLKSYFYSSERGLPGATILYLTENSNRQRS
                     WDRTFFTQAHYEKKISSLWSFGFNAKYHYGFLKYLDPDVNNSLGKIENIYRQQEYYSS
                     FSFLFQAFENLSFSSSTDIACNTLSANLTDFAYPTRWTLLSCLNGKYVNHFLLTTAGL
                     LLTNVNDKVRYGKAPENYQQLSPYASISVKPFNKYDVRFRCFYKNIFRMPTFNDLYYV
                     YVGNRDLKPEKSHQFNAGVTYCTSLENWLPLLTITWDVYHNEVKNKIIAFPQKDIFNW
                     TMKNYGKVLIDGLDLSVEGSFSLSEKIGIIAGTTYTYQKAINVTDPSEENYRHQIPYT
                     PRVSGSGRAALQTPWLNIAYSFLWSGKRYALFQNYAENRLSGYADHSISLQKEIKIKQ
                     QVLSLNAEILNLWNTNYEIIRWYPMPGRSFRITVATRF"
     regulatory      complement(2333..2556)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2673..3410
                     /locus_tag="PLR72_04430"
     CDS             2673..3410
                     /locus_tag="PLR72_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOK36483.1"
                     /translation="MAGYGKWLGGGLGWAFAGPIGALIGFAIGSIIDAAATKSAPTTK
                     KTFYQTTEGDFKASLLVLVVSVMKADGSVKKSELNVVKRFLVANFGEEAALDALQILK
                     KLLEKEIDIAPVTAQINQYMNYPIKLEILHLLLEIAYADAEVSSSEMNAIQRIAYLLK
                     ISSSDFESLKAIYVKHEDHQWAYKALEIESTASNEEIKKAYREMAKKYHPDKVNTLGE
                     DIKKIATEKFRSINEAYEVLKKERGFA"
     gene            3449..4918
                     /gene="cysS"
                     /locus_tag="PLR72_04435"
     CDS             3449..4918
                     /gene="cysS"
                     /locus_tag="PLR72_04435"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOK36484.1"
                     /translation="MENQLYIYNTLHRQKELFVPLQPPHVGMYVCGPTVYGDPHLGHA
                     RPAITFDILFRYLKHLGYKVRYVRNITDVGHLENDVDSGEDKIAKKARLEQLEPMEVA
                     QYYMNRYHKAMEALNVLPPSIEPLASGHIMDQIDFVEKILKNGFAYVSEGSVYFDVEK
                     YDKIYHYGKLSGRNIADLLEATRSLEGQEEKRKSYDFALWKKASPEHIMRWKSPWSEG
                     FPGWHMECSAMSTKYLGKHFDIHGGGMDLIFPHHECEIAQSTAALGSESVKYWMHNNM
                     VTINGQKMGKSLGNFITLEEFFTGNHPLLTQAYSPMTIRFFMLQAHYRSTVDFSNEAL
                     QASEKGLQRLLEGYERLQKIQPSEKSTVDLSDLRQNCEADMCDDLNTSVVISHLFEAV
                     KTIYLINDEKETISSDDLKEVKDVFHLFIEEILGLKTENADSSKLEAYKKAVDLLLNI
                     RLEAKKNKNWELSDKIRSELTALGFEIRDTKDGFEWKLN"
     gene            5015..5368
                     /gene="rbfA"
                     /locus_tag="PLR72_04440"
     CDS             5015..5368
                     /gene="rbfA"
                     /locus_tag="PLR72_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOK36485.1"
                     /translation="MQVTRQQRIEKLIQKELGEIFLLYAREIPGTIIGVTNVRISPDL
                     SIARIHISIYPTNKSGEIFENINHENKSVRFELGKRVRNQLRIIPELHFYLDDSLDYA
                     EHIDQLLKGNIPPKQ"
     gene            5416..6696
                     /locus_tag="PLR72_04445"
     CDS             5416..6696
                     /locus_tag="PLR72_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK36486.1"
                     /translation="MSSKKLQASDTSGKFMFSLWIAKRYLFSKKSHNAINVISAISAI
                     GVAIGTMALVCVLSVFNGFESVISDMFSAFDPDLKITVTQGKTFDITTPAWQEVKKIK
                     AIDCFTEVIEENALLRFKDKQMPAVIKGVSDDFERMTRIDSIMYDGKFILFDGSFQRA
                     VIGIGIANILGLGAHFIDPLYIYAPKRTSDINLLRPETSFNQVGTFVSGIFAVNQLEY
                     DDQYVIVSLALARDLFEYDSTKVSAVELKLANLSHQKETQKQIKSILGPNFQVKNRYE
                     QQESFFKIMKIEKWITYLILCFILLIACFNIIGSLSMLIIEKKEDIRMLRNLGADQRL
                     IKRIFLFEGWLISIIGALLGIILGVVLCLLQQHFGFLKLGSGYVLNAYPVIVQFIDMV
                     LVFITVLLMGFIAAYYPVRYIRVDENLRENNMEY"
     gene            7233..7889
                     /locus_tag="PLR72_04450"
     CDS             7233..7889
                     /locus_tag="PLR72_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36487.1"
                     /translation="MKTYIKKFFYFPVFLSFLLAACINNEPKEPYIYEKNPQYTWGYA
                     VFYGDYYAEYGNENNVLSISLFTNGLGINENNYLIGTGQYLYLEDIFVTPTDTLLPDG
                     IYTIDKSGESFTISPGEHLSIDGVIYTVGATITYLDIYLLNPVEKLITEGTMNVSRQD
                     TIYTISCNFKTDDNLELKGNFTGILPHFDQSLKKNSESLRMLRHTKRSFILSRLNSSK
                     "
     gene            8052..8993
                     /locus_tag="PLR72_04455"
     CDS             8052..8993
                     /locus_tag="PLR72_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOK36488.1"
                     /translation="MNYQSIPPKQPGLSQFFNIDLREIVPFIHWSFFFREWRIPGKFE
                     EIEKVSSCVSCKQSWLQNFSGSARDKAEEALKLFQDAQNMLQQFSVEKIVQINAAVGL
                     FSAFAQEENIHILHENKEFIIPTLRQQHPSTDGFCYALSDFLNVENDYIGTFATVVQG
                     TEKLIQHFENEKDIYSSMMVKTLANRLAEATAEWLHYQVRTKIWGYAPDEPLEIENIL
                     KNKYQGIRPAIGYPSLPDQSIIFDLEPLLQLNTIGIKVTENGAMYPPPSVAGLYFAHP
                     QSKYFNVGKIDDQQLKDYARRRGKTIEEMKKWLAANI"
     gene            9047..9265
                     /locus_tag="PLR72_04460"
     CDS             9047..9265
                     /locus_tag="PLR72_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOK36489.1"
                     /translation="MLNIKSELLKIKGMSCSHCAARVEKALNTIDGVSAKVDLNSNTA
                     KVSLLKEVSDEALKKAVEDVGYEVTGIL"
     gene            complement(9283..>9413)
                     /locus_tag="PLR72_04465"
     CDS             complement(9283..>9413)
                     /locus_tag="PLR72_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_091910270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOK36490.1"
                     /translation="NEFCYKFNRRYFGDAIFDRLLVAAVSYNSDFKSRIYNRNHCG"
BASE COUNT         3252 a         1412 c         1655 g         3094 t
ORIGIN      
        1 attaaagtaa agcgaaatta cgggaaccac taaaaatgaa atgttttttg aagcaaaata
       61 gtaagtatgg attggagcaa aacccatttt ataattgtca tacaagataa taacgtttct
      121 caatgtaaca aaaaagagaa aatttctcaa aatctcaata aaaaaatttt ttgcttgttt
      181 cattccgaaa ataacattta cttttttctc aaaatcgagt agctacggtg atacggaatg
      241 aacgaccagg catgggatac caccgaatta tttcataatt cgtattccat aaattcaata
      301 tttctgcatt cagcgataaa acttgctgct tgatttttat ttctttttgt aaggaaatac
      361 tatgatcggc ataaccagac aaacgatttt ctgcataatt ttgaaaaagt gcatatcttt
      421 tacccgacca gaggaacgaa taggcaatat taagccaggg cgtttgcaag gcagctcttc
      481 ctgaaccaga aactcttggt gtataaggga tttgatgacg ataattttct tctgatggat
      541 cagttacatt aatagctttt tggtaagtat aagtagttcc tgcaattatt ccaatttttt
      601 cggataacga aaacgaacct tctacggata aatccaaccc atcaatcaaa acttttccat
      661 aatttttcat tgtccaatta aaaatatctt tttgtggaaa agcaataatc ttgtttttca
      721 cttcattgtg ataaacatcc catgttatgg tcaacaatgg cagccagttt tcaagagaag
      781 tacaataagt aacacctgca ttgaattgat gacttttttc aggctttaaa tctcgattgc
      841 caacataaac ataatataaa tcgttgaaag taggcatccg aaaaatgttt ttataaaaac
      901 accgaaaacg tacatcatat ttgttaaaag gttttacaga aatacttgca taaggagaaa
      961 gttgctgata attttcgggt gctttaccat atcgaacttt atcatttacg ttggttaaaa
     1021 gcaaaccagc ggtagtcagt aaaaaatgat ttacatattt tccattcagg caggaaagta
     1081 aagtccaacg tgtaggataa gcaaaatcag ttaaatttgc agagagagta ttgcaagcaa
     1141 tatcggttga agaagaaaac gacaagtttt caaaagcttg aaaaaggaaa ctgaaagaac
     1201 tgtaatattc ttgctgtcga taaatatttt ctatttttcc caaactgttg ttgacatcag
     1261 gatcaagata ttttaaaaaa ccatagtgat attttgcatt gaaaccgaaa gaccacagcg
     1321 aggatatttt tttttcataa tgagcttgtg taaaaaatgt tctgtcccaa cttcgctggc
     1381 ggtttgagtt ttcggtaaga tacaaaatag ttgctcctgg caatccacgt tcagaagaat
     1441 aaaaataact tttcaaatag cctgaactat tttcattttc cttataataa agagctcctt
     1501 ctaatctgaa attttttaca tcggtatttt ttcttttttc ccatgaagtg ctgtcattga
     1561 ctcctgtgcc atattttaat agataaggat attttccatt tgctgaaagc cattcgctgc
     1621 tcactacagc agaaaattgc tgattaattt ttccttgaaa aaagaaaaaa ggatttacta
     1681 aaccaaatga accgcttttg aaggaaattt ttccattaaa tttttctttt tctttaaaat
     1741 agggggcaga agtctgaatg tttaatacag aagcagaagc aaaaagacgt gctggctgaa
     1801 atatattatc gttttgacca ttgttaaatg tcagtaaatc aacattttca atagagaatc
     1861 ttcctaaatc aatttgtcca gtttgacaat cagaaagtgt gatgccgtca taacttactg
     1921 cggtatggtt ggcatccaac ccacgtactg aaacagtttt caaaccacca attccgccgt
     1981 aatcttttac tgaaataccc gaaaaatatt ttactgcatc cgacacctgc aatacattca
     2041 aattttttat ttgttcactg gttaatattt gcaacggggt cgacgatctg acttcgggtg
     2101 gctttattct tccagtaaca acaatttctg gaattaaata aatttttgtg gtgtcaataa
     2161 ctggatttgc tttgaccttt gaaatattgg gaaacaatga acaattaaaa atcagaaata
     2221 taaaaatttt tgttttcctg tttttgttct tttttgtaaa tagctgaata aaattgatga
     2281 tgttgtgtaa cacagattat tgattttata gcttctatcc gcggaagctt ttaattgatt
     2341 caaaaataca ggcaggtctt ctggcttact cttttttgaa cgccttccca agtttcaatg
     2401 aattttcagt cgtccattga gattatcagt ggcaaaagaa atgttcaaaa agttttatag
     2461 agatcacagc agcgggactg caaccgattt tcacgatttt ccctcttcgt caatttctta
     2521 gctctaaaaa attgaacctg taccttgcaa aattacttaa aataatttgg atttatgata
     2581 tgtgaaaaat agtaatttca ttttcaattt ggtattttcc taatttagcg ttcttttttg
     2641 taattttgta aattctctaa agttgataaa ttatggctgg atatggaaaa tggttgggag
     2701 gcggtttagg gtgggctttt gcaggtccta ttggggcttt gattggcttt gccattggat
     2761 ctattattga tgctgctgca acaaaatctg ctccaacaac aaaaaagact ttttatcaaa
     2821 ctacggaagg agattttaaa gccagtctgc tcgtgttggt tgtcagtgtg atgaaagcag
     2881 atggaagtgt taaaaaatca gaattaaatg tggtgaaacg ttttctggta gcaaattttg
     2941 gtgaagaagc tgcattggat gccttacaaa tattgaaaaa attattagaa aaggaaattg
     3001 atattgctcc agtcactgca caaataaatc agtatatgaa ttatcccatt aaattagaaa
     3061 tattacattt gttgctggaa attgcttatg ctgatgctga ggtttcttct tcagagatga
     3121 atgcgattca aaggattgcc tatcttttaa aaatttcttc ttctgatttt gaatcactaa
     3181 aagcaattta tgtcaaacat gaagatcatc agtgggctta taaagcactt gaaatcgaat
     3241 caaccgcttc taatgaagag attaaaaaag catacagaga aatggcaaaa aaatatcatc
     3301 ccgacaaagt taatacgctg ggagaagaca ttaaaaaaat agcaactgaa aagtttcgtt
     3361 ccatcaatga agcttatgaa gttttaaaga aggaaagagg ttttgcttga aaaaatttat
     3421 aataattaga agaaacaaat gataacttat ggaaaatcaa ttatacattt ataacacttt
     3481 acatcgacag aaggagttgt tcgttccact tcaaccgcca catgtaggaa tgtatgtttg
     3541 cggacccact gtttacggtg atccccatct gggacatgca cggcctgcca ttacttttga
     3601 tatcctgttt cggtatttaa aacatttagg atacaaagtt cgttatgttc gtaatattac
     3661 agatgtaggt catctggaaa acgatgtcga cagcggtgaa gacaaaattg ctaaaaaagc
     3721 acgactggag caattggagc caatggaagt tgctcaatat tacatgaatc gctaccataa
     3781 agcaatggaa gcattaaatg tattaccccc cagcattgaa ccccttgctt caggacatat
     3841 catggatcag attgattttg ttgaaaaaat tctaaaaaat ggttttgctt acgtaagtga
     3901 aggttcggta tattttgatg tggaaaaata tgacaaaatt tatcattatg gcaaactttc
     3961 agggcgaaac atagctgatt tgttggaagc aactcgctcg ctggaaggac aagaagaaaa
     4021 aagaaaaagt tatgattttg ctttgtggaa aaaagcttca ccagaacata tcatgcgatg
     4081 gaaatcgccg tggagtgaag gttttccggg ttggcacatg gaatgttcag ccatgagcac
     4141 gaaatattta ggcaaacatt ttgacatcca tggtggagga atggatttga tatttcccca
     4201 tcacgaatgt gaaattgctc aatctacggc tgctttagga agtgaaagtg taaaatattg
     4261 gatgcacaat aacatggtga ctatcaatgg tcagaaaatg ggaaaatcgt tgggaaattt
     4321 tattacactc gaagaatttt ttactggcaa tcatcctttg ttgacacaag cttacagtcc
     4381 aatgactatt cgttttttca tgctacaagc tcactaccgt agcacagttg atttcagtaa
     4441 tgaagcttta caagcttcag aaaaaggttt acagcgttta cttgaaggtt atgaacgttt
     4501 gcaaaaaatc cagccttccg aaaagtcaac agtggatctt tctgatttgc gtcaaaattg
     4561 tgaagctgac atgtgcgacg atttgaatac atcggtggta atttctcatt tgtttgaagc
     4621 agtgaaaacc atttacctca ttaatgatga aaaagaaaca atttcttcgg acgatttgaa
     4681 agaagtaaaa gatgtttttc atctttttat tgaagaaata cttggtttaa aaacagaaaa
     4741 tgccgattca tcaaaactgg aagcttataa aaaagcggtg gatttacttt taaatatccg
     4801 actggaagct aaaaagaaca agaactggga attgagcgac aaaattcgta gtgaattaac
     4861 agctttgggg tttgaaatca gagacaccaa agatggtttt gaatggaaat taaattaaat
     4921 tgttattaag agagaaggat atcttctcaa aaagagaaaa aaattacgtt tcatttttaa
     4981 aatgacgctg aaaggcaaat taaaaattaa aaaaatgcaa gttacccgac aacagcgaat
     5041 agaaaaactg atacaaaagg aattggggga aattttcttg ttatatgcca gagaaattcc
     5101 aggaactatt ataggcgtta ctaatgttcg aattagtcca gatttaagta ttgctcgtat
     5161 tcatatcagt atttatccaa ctaataaaag cggtgaaatt tttgaaaaca tcaaccatga
     5221 aaataaatct gtacgctttg aattgggaaa aagagtcaga aatcaattac ggattattcc
     5281 tgaacttcat ttttatctgg acgattcact ggattatgcg gaacatattg atcaattact
     5341 gaaagggaat attccaccaa aacaataaat ctcagtaaat tttccctgaa aagtttttcg
     5401 aaaaaagctg aaaacatgag ttcaaaaaaa ttgcaagcaa gtgatacttc tggaaaattt
     5461 atgttttctt tgtggattgc caaacgatat ttattttcta aaaaatcaca caatgcaatt
     5521 aatgtcattt cagccatttc agcaataggt gtggcaattg gaacaatggc tctggtatgc
     5581 gtattgtcag ttttcaatgg ttttgaatcc gttatttccg acatgttctc ggcttttgat
     5641 cctgatttga aaattaccgt cacgcaagga aaaacttttg atatcactac acctgcatgg
     5701 caggaagtaa aaaagataaa agctattgac tgtttcacag aagtaattga agaaaatgca
     5761 ttattgcgtt tcaaagataa acagatgccc gctgtcatta aaggtgtaag tgacgatttt
     5821 gaaaggatga caaggattga cagcatcatg tatgatggta aatttatttt atttgatgga
     5881 agttttcaaa gagcagttat tggaattgga atagccaata ttcttggttt aggagctcat
     5941 tttattgatc cattgtatat ttacgcacca aaaagaactt ctgatataaa tttgcttcga
     6001 cccgaaacca gcttcaatca ggtaggaact tttgtatcag gaatttttgc agtcaatcag
     6061 ttggaatatg acgatcaata cgtgattgtt tctcttgcat tggctcgtga cttatttgaa
     6121 tatgattcta ctaaagtttc ggctgtagaa ctaaaacttg caaatctttc ccaccaaaag
     6181 gaaacacaaa agcaaataaa atctatatta ggacctaatt ttcaagttaa aaatcgctat
     6241 gagcagcagg aaagtttttt taaaattatg aaaatagaaa aatggattac ttacctgatt
     6301 ctttgtttta ttttgttgat tgcctgtttt aatattattg gatcgctatc aatgttaatc
     6361 attgaaaaaa aagaagatat ccgaatgttg cggaatttgg gcgctgatca acgtttgatt
     6421 aaacgaattt ttctttttga agggtggctt atttctatca ttggagcttt gttaggtatc
     6481 attttgggag tagtactttg tttgctgcaa caacattttg gttttttaaa attaggttca
     6541 ggttatgtat taaatgctta cccagtgatt gttcaattta tagatatggt tctggtattt
     6601 attactgttc ttttgatggg ttttatagca gcttattatc cagtgcgata cattcgagta
     6661 gatgaaaatt tacgagaaaa taatatggag tattaatttt tttatatttc acccatatac
     6721 ctactgatat tttaatgtta caaagacaga tgaaaatatt ttcattagga aattgcaagg
     6781 aattcgattt ttgcttaaaa gtttgacaaa aattgctaca tttgttactt atagaggaat
     6841 aaaattcatt aatttaaaca aataaaaaag tagattttac cttattataa atacgaagca
     6901 aattaataaa gaaaccttat cttacttaaa accttaataa caaataatca acgagttaaa
     6961 caaaccttat taccttattt aaaacaaatt aaaaacacaa atcgaaagaa agtaacaatt
     7021 ttatctaaat aaagtaataa ggttggaaga aagggaaatt ttagtgttat aaaggttata
     7081 tgttttaata aggtttaaaa gacaaaataa ggaaactgac ttcaaacaaa tgaacacaaa
     7141 catatatttt aagtatgaga atgaaatttt ttaatttctt acatgttccc acataattga
     7201 tattgtaaaa acttcataaa atttattttc ggatgaaaac atatattaaa aagtttttct
     7261 attttcctgt ttttctatca tttttactgg ctgcttgtat taataatgaa ccaaaagaac
     7321 cttatatata cgagaaaaat cctcaatata catggggcta tgcggtattt tatggtgatt
     7381 attatgctga atatggaaat gagaataacg tgttatctat ttcgctgttt accaatggat
     7441 tgggtattaa tgaaaacaat tatttaattg gaacgggtca atacttgtat ctggaagata
     7501 tttttgtaac accgaccgat actttgttac ctgatggtat ttatactatt gataaaagtg
     7561 gagaatcttt tactatttct cctggtgaac atttgtcaat agacggcgtc atttatacag
     7621 ttggagcaac cattacctat ttagacattt atttgctgaa tcctgtcgaa aaattaatta
     7681 ctgaaggaac aatgaatgtt agtcgtcaag atactatcta tactattagt tgcaatttta
     7741 aaacagacga caatcttgaa ctaaaaggaa acttcactgg tatattgcca cactttgatc
     7801 aatcgttgaa gaaaaattca gaaagtttac gaatgttaag gcatacgaaa cgttctttta
     7861 tcttatccag attaaattct tcgaaataaa aaggcttttc tttctgattt ttaactttta
     7921 tttttgttta cttgtagata aaaagactgt ttaaattttt tataaatgtc taaaatataa
     7981 ttttcagtga catttcttca acaaattggg tatccctttt cgttaattca aaatttattt
     8041 tgagaaaaga gatgaattat caatccatac caccaaagca accaggatta agtcagtttt
     8101 ttaatattga tttacgagaa attgtacctt tcattcattg gagttttttc tttcgcgagt
     8161 ggcgaattcc tggaaaattt gaagaaattg aaaaagtttc ttcttgtgtt tcttgcaagc
     8221 aatcgtggtt acagaatttt tccggttcag ctcgtgataa agcagaggaa gccttaaaac
     8281 tttttcagga tgcccaaaat atgcttcaac agttttctgt agaaaaaatt gttcagatta
     8341 atgctgcagt aggcttattt tctgcctttg ctcaagaaga aaatatccat attttgcatg
     8401 aaaataaaga attcatcatt cccacacttc gtcagcagca tccttccacg gatggttttt
     8461 gctatgcttt atctgatttt ttgaatgttg aaaatgatta tattggtact tttgctacag
     8521 tagtgcaagg gacggaaaaa ttgattcaac attttgaaaa tgaaaaagat atttacagtt
     8581 ccatgatggt gaaaacttta gcaaatcgtt tggcagaagc tactgctgaa tggctacatt
     8641 atcaggtacg aacaaaaatt tggggttatg ctcctgatga accgctggaa atagaaaata
     8701 ttttgaaaaa caaatatcaa ggtattcgtc ccgctattgg ttatccttct ttaccagatc
     8761 aatctattat ttttgacttg gaacctttgt tgcaattgaa tacaattggc ataaaagtaa
     8821 cggaaaatgg agctatgtat ccgccaccgt cagtagcagg attgtacttt gctcatccac
     8881 aatcaaaata ttttaatgta ggcaaaattg atgatcaaca attgaaagat tatgcacgtc
     8941 gacgtggaaa aacaatagaa gaaatgaaaa aatggttagc tgcaaacatc taattgttct
     9001 atatcttgaa aaacaaaatc aatttaatga tttttgaact tgtaaaatgt tgaatatcaa
     9061 aagtgaatta ttaaaaatca aaggaatgag ttgctctcat tgtgcagctc gggttgaaaa
     9121 agcgttgaat acaattgatg gcgtttctgc aaaagtagac cttaattcaa acactgcaaa
     9181 agttagctta ttaaaagaag tcagtgatga agctttaaaa aaagcagttg aagatgtagg
     9241 ttatgaagta acaggcattt tataaaaaat tttcttttat gattatccgc aatgattcct
     9301 attataaatt ctcgacttga aatcggagtt gtagcttaca gcagcaacaa gcaacctgtc
     9361 gaagatggca tcaccaaaat agcgtcggtt gaatttataa cagaactcat tga
//
LOCUS       DAOSAS010000069         9244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_4897_length_9244_cov_13.101861, whole genome shotgun sequence.
ACCESSION   DAOSAS010000069 DAOSAS010000000
VERSION     DAOSAS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4897_length_9244_cov_13.101861"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..2201
                     /locus_tag="PLR72_04470"
     CDS             240..2201
                     /locus_tag="PLR72_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOK36491.1"
                     /translation="MKDKFSFLKNTDTFSHIVSLKYLPRWIVLLLDVLICCLSFLFAY
                     LITSFFVSDSPDIRVTSFFQRLVLGLSIQIIFFWFFHTYAGILRYSNFTDAIKLLLAV
                     FSNFIVLVIINFIYTKVYSDLLFTYSELIIYAIIAFLFLFLERLFIKVTYDFFVENSS
                     NLVPVMIYGTDPAGISIGKMILSKVGDDNTKYKLVGFIDNDHNTRQKILLGVNVYPYD
                     KNTIATKIPKKAKTIIVSPAKLKEIGFKEDLDVFINNNLSVLTSAPTDLWENEIPNVK
                     QIRSIKIEDLLGREPIHISTTNIENQLKSKIILVTGAAGSIGSEIVHQLVQYQPLLIV
                     LVDNAETPLYELKIELQEKYPQQNFSFFLGDVRDQERMENILDLHKPDIIYHAAAYKH
                     VPMMEDFPTESVQVNVKGTKVMADLAVKYKVKKFVMISTDKAVNPTNVMGASKRIAEI
                     YVQSLNKKLQKNGHETTKFITTRFGNVLGSNGSVIPHFQKQIEKGGPVTITHPDIIRY
                     FMTISEASNLVLEASAIGEGGEIYIFDMGEPVKISDLARKMIRIAGYVPDVEIKIVYT
                     GLRQGEKLYEELLNQKEKTIPTQHPKIMVAKVIEYEYDEISLEIEKLLQYSSLNKDFL
                     IVQQMKKIVPEFKSNNSQFEFLDILKKSQ"
     gene            2293..3387
                     /locus_tag="PLR72_04475"
     CDS             2293..3387
                     /locus_tag="PLR72_04475"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOK36492.1"
                     /translation="MNSIVHFIQQYPFLTIIISFIIGKLFMPVAIKIAKKHNFLVKPN
                     KRTSHKGLIPYTGGLDIFVSFFLTMILFSYEIVAQTQFIILGTLTIFFIGFIDDLITL
                     KPMAKLSSEIIAGIFLIIIANIRISHFHGILGINELPLIMSYLFSLFTFIVIVNALNL
                     IDGVDGLASGLGIIYNLFFGIYFNLSGYLYLALAAYTLAGSLIVFFIYNVFGKKNKIF
                     MGDTGALFLGFMITFFVFEFCEINAYHKVPEAYHMSAAPAVAICLIIVPLFDTFRVML
                     TRIKKGFSPFKADKNHIHHLLLKIGFSHRQVTLILLTSTIFFIFLALIGRNWNNLLLI
                     FIALLIASSLTYYLWRVINKKMQRKTIVNI"
     gene            4030..5055
                     /locus_tag="PLR72_04480"
     CDS             4030..5055
                     /locus_tag="PLR72_04480"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOK36493.1"
                     /translation="MISKIIAEELIEKVYEAINEVDKIAILVHVDPDGDAIGAALGLW
                     HYLMTIEKEPVVISPTSFPNFLSWMPGSKQILIYENDKTSVEKALASAELIFGVDFND
                     RRRLEKMSELVSKILVPTILIDHHLQPEKFAKITISYPEISSTSELIFRLICRMGDFS
                     KINLACAECIYTGMMTDTGNFTYNSNDPEIYIIISELIKIGIDKDEIYRKVFNTYSEN
                     RLKLLGYCLYKKMKIFSTYRTAIISLSQQELIAFNYTNGDAEGFVNIPLSIEGIDFSV
                     LLREEKDKIKISLRSQGNFPANKVAADLFGGGGHLNAAGAESYTSLEETEKQLLNALP
                     DYLIFFD"
     gene            5190..6407
                     /locus_tag="PLR72_04485"
     CDS             5190..6407
                     /locus_tag="PLR72_04485"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOK36494.1"
                     /translation="MKYVVILGDGMADEPIDSLGNKTPLQVANKPTIDKIAALGRSGM
                     LNTIPEGFSPGSEIANLSVLGYDVADVFEGRGSLEAASMGVSLEKGEMAMRCNLICIE
                     NEKIKNHSAGHISNEEAAELIEFLNNKLGNENISFYHGVSYRHLLKMRGGNKNLKCTP
                     PHDVPGIPFRKVMIKALSPDAQATADYLNELTLRSQELLENHPVNLKRAAEGKDKANS
                     IWLWSPGYKPEMKTLSELYHFKKGSVISAVDLIKGIGVYAGLNVINVEGATGLYNTNY
                     EGKAQAAIEALRTDDFVYVHVEASDEAGHDGDVPLKIKTIEYLDARIVKPIFEEVSKW
                     NEPVTIAVLPDHPTPCALRTHTCDPVPFVIYHPGETPDEVTTYDEFAARKGSYGLLKG
                     NEFMKNLIGNSNL"
     gene            6630..7952
                     /gene="thrC"
                     /locus_tag="PLR72_04490"
     CDS             6630..7952
                     /gene="thrC"
                     /locus_tag="PLR72_04490"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOK36495.1"
                     /translation="MKYYSTNKIVKNITLQEAVVKGLADDKGLFMPEEIKKLPSSFFD
                     TMMNMSLQEISFIVADAFFGDDIDSRALKKIVDDTLNFDIPLIPVEDHIYSLELFHGP
                     TLAFKDVGARFMSRLLSYFIQKEGERNVNVLVATSGDTGSAVANGFLGIEGIHVYVLY
                     PKGLVSSIQECQFTTLGENITALEIEGTFDDCQRLVKSAFVDPELNACFKLTSANSIN
                     IARLLPQSFYYFYAFAQLRAIHPKYTAEELEMVICVPSGNFGNLTAGLIAKRMGLPIK
                     RFIAANNRNDVFLEYLQTGEYHPRTSVATIANAMDVGNPSNFDRILDLYGYSHEAITK
                     DISGISYTDEQIAATIKNCFSKNGYLLDPHGACGYQALKDKLLPEENGIFLETAHPAK
                     FLDTVEKIIGEKIEIPAKLQEFMKGEKKSIPLSKDFEEFKKFLLANAH"
     gene            complement(8161..9174)
                     /locus_tag="PLR72_04495"
     CDS             complement(8161..9174)
                     /locus_tag="PLR72_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36496.1"
                     /translation="MKKNNISIKKIIVPTIPLGGFLILLCLGLWIISFFIKTNPPSLS
                     SLSPLMATLEENLPVNIVIANILSFVLTIFNGMLAEHLNNRFTIIHARTFLPFFIFLM
                     LMSTWYGIHSMWILQIVLTFFIGSFFFLFDMFHQNQPVEQAYIGSLLIGLCSLFLPSL
                     IFIIPLFWVGFFQFQNFTLKTFLASLLGWLTPFVLYFGFYFVFQPQVDLYPLFVQYFH
                     FESNFSFKPIMSIESIYGGLFALLLLVIIGSIYNDFQKNAIPTRKRLNFLVFSLLLFI
                     VLSLLNFYQRIFYLPFFAYISSLLFSHAFTLKENKFFSVLFIVFCLLNIAFVFSKLLI
                     DFG"
BASE COUNT         3283 a         1352 c         1475 g         3134 t
ORIGIN      
        1 aatgatttaa tagcataaaa ataggttgaa aaatcaattt tctggaattg gagtatttta
       61 gtaactaatt gcaataaaaa ttcactgttg tttttaaaat catttgagat atctttatat
      121 agataaaatt caaaataaaa aacatacaga aattgaagct atatttaata ttttaaacgt
      181 atatttgtaa actattttaa agggcaaatg agttttaact acgattaaat tttcaaaaaa
      241 tgaaggataa atttagcttt ttaaaaaata cagatacttt tagtcatata gtttctctca
      301 aatatttacc acgttggata gtattattat tagatgttct tatttgttgt ctttcctttt
      361 tatttgctta tttaattact tccttttttg tatcagattc tcctgatata cgagttactt
      421 ctttttttca gcgattagtg ttggggttaa gtattcaaat aatatttttt tggttttttc
      481 atacttatgc tggtattctt cgatattcaa attttaccga tgcgattaaa ttgttattag
      541 ctgtttttag taattttatt gttctcgtaa ttataaattt tatttataca aaagtttatt
      601 ctgatttact ttttacttat tccgagttaa ttatttatgc catcattgct tttctatttc
      661 tttttttaga aaggttgttt attaaagtaa cttatgattt ttttgtcgaa aatagtagca
      721 atttagtgcc agtaatgatt tatggtaccg atcctgcagg tatcagtatt ggcaaaatga
      781 ttctttccaa agttggtgac gataatacca aatataaact tgtgggattt attgacaacg
      841 atcataatac ccggcaaaaa atactattag gagtgaacgt ttatccttat gataaaaata
      901 ctattgctac aaaaattcct aaaaaagcaa aaaccattat tgtttcacct gccaaattaa
      961 aagaaattgg attcaaagaa gacttagatg tttttattaa caacaatttg tcggttctta
     1021 cttctgctcc taccgacctc tgggaaaatg agatacctaa tgttaaacaa attcgttcca
     1081 taaaaataga agatttgctg ggaagggaac ctattcatat ttctactact aatattgaaa
     1141 atcaattaaa atctaagata attcttgtca ctggtgccgc aggttcaata ggtagtgaaa
     1201 ttgttcatca acttgtacaa tatcaacccc tcttaatcgt tttagtagat aatgcagaaa
     1261 cgccattgta tgaattaaaa atagagcttc aagaaaaata tccgcaacaa aattttagct
     1321 ttttcttagg ggatgtccga gatcaagaac ggatggaaaa tattcttgat ttgcataaac
     1381 ctgatattat ttatcatgca gcagcttaca aacatgttcc tatgatggaa gattttccga
     1441 cagaaagtgt tcaagtaaat gtaaaaggga ctaaagttat ggctgattta gcagtaaaat
     1501 acaaagttaa aaagttcgtt atgatttcaa cagataaagc ggtcaatcca accaatgtaa
     1561 tgggagcttc gaaacggatt gccgaaattt atgttcaatc actgaataaa aaacttcaga
     1621 aaaatggcca tgaaacaact aaatttataa ctacacgttt tggaaatgtt ttaggttcaa
     1681 atgggtcagt aattccccat ttccagaaac aaattgaaaa aggaggacct gtaaccataa
     1741 ctcatcctga tattatacga tattttatga ccatttctga agcgagtaat ctggtgcttg
     1801 aagcaagtgc aataggagaa ggaggagaaa tatatatatt tgatatgggc gaacctgtaa
     1861 aaattagtga tttagctcga aaaatgatta gaatagctgg ttatgtccct gatgttgaaa
     1921 tcaagattgt atatactggc ttaagacagg gtgaaaaatt atatgaagag ttattgaatc
     1981 aaaaagaaaa aactatacct acacaacatc ctaaaattat ggttgctaaa gtcattgaat
     2041 atgaatatga tgagatttca ctggaaattg aaaaattact tcagtatagc tctcttaaca
     2101 aagattttct gatagttcaa caaatgaaaa aaattgtacc tgaatttaaa agtaataatt
     2161 cacaatttga atttttagat atccttaaaa aatcccagta attaacaaat attttcttaa
     2221 ttatccttct tttttaaaat gttatatttt tttcatactt tccgtttaat cacgcataaa
     2281 tcaatttaga aaatgaattc cattgtacat tttattcaac aatatccttt tcttactatt
     2341 attatttctt ttattattgg gaaattattt atgcccgttg ctattaaaat agccaaaaaa
     2401 cataattttt tagttaagcc taataaacgt acttcacaca aaggcttaat tccttatacg
     2461 ggtggacttg atatttttgt ttcttttttc cttacgatga ttcttttttc ttatgaaatt
     2521 gtagctcaaa ctcaatttat aatcttaggc actttaacta ttttttttat tgggtttatt
     2581 gatgatttga ttaccctgaa accaatggca aaattaagta gtgaaataat tgctggaatt
     2641 tttttaatta ttatagcaaa tattagaatt tctcattttc atggaatttt agggattaat
     2701 gagttacctc ttattatgag ttatttattt tcacttttta cttttatagt tattgtaaat
     2761 gctttgaatc taattgatgg agtagatggt ttggcttctg gtttgggaat tatctataat
     2821 ttattttttg gcatctattt taatttatca ggctatttgt atttggcttt agcagcttat
     2881 actcttgccg gctcattgat tgtatttttt atttataacg ttttcggtaa aaaaaataaa
     2941 atctttatgg gtgatactgg agctcttttt ttaggattta tgataacttt ttttgttttt
     3001 gaattttgtg aaataaatgc ttatcataaa gttcctgaag cttatcatat gtcagcagct
     3061 cctgcagtag ccatttgttt aattattgtt cctttgtttg atactttccg agttatgtta
     3121 accagaataa aaaaaggttt ttctcctttt aaagctgaca aaaatcatat tcatcatttg
     3181 cttttaaaaa ttggtttttc tcatcgtcaa gttactttaa tattgcttac ctctactatt
     3241 ttttttattt ttttagctct cattggacga aattggaata atctgttatt aatttttatt
     3301 gctctattga ttgccagcag tttaacctac tatctttgga gagttattaa taaaaagatg
     3361 caacgaaaaa ccattgtaaa tatttaacat tttatttgaa tcttttctcc attataaaat
     3421 aaggtttcag ttgatcatct aagtattgaa tctgttgtcg acatctttgt tcggatgtaa
     3481 attttttaat tcatccttcc ccaaaatgat attattccaa ataagtgtgt gaagttcaaa
     3541 tttttttctc ttataataaa ttgttctttt tgttattgta ctaaaacaat tatccgtctg
     3601 ttagaaagct atttttcctc cgagtttgct gcctgttttc atagaagggt accttaaaat
     3661 tttttacata cgattcgata aattcagctt acttaaataa tttaccgttt tgagcttgct
     3721 tacaaaaatg cttatactaa agtactatca attggtatta tatagggttt ttttaataaa
     3781 gataggcaga aaaaggacat aaaaaatgct gcttaaaaat tttcttttct tcgaaaaatt
     3841 acatttaatt tgtgaagaaa gacaagtcta aaagttcaat gattacaaac gcttaaaagc
     3901 tggttaagat aaccagtttt tttacgatta ttggaaaatt agatgcgtca attctgtgat
     3961 ggtattaatt aaaattctcc tacttatatt tttttactac ttttgcatct atttttaaaa
     4021 cctaaataaa tgatttcaaa aattattgcc gaagaactga ttgaaaaagt ttacgaagct
     4081 attaatgaag tggataagat tgccatattg gtccatgttg atcctgatgg tgatgctatt
     4141 ggagctgctt tagggttgtg gcattatttg atgaccattg aaaaagaacc ggtagttatt
     4201 tctcctactt ctttcccaaa ttttttaagc tggatgccgg gcagcaaaca aatattgatt
     4261 tatgaaaatg ataaaacttc tgtggaaaaa gctttagctt ctgcagaatt aatttttgga
     4321 gttgatttta acgatcgccg ccgcttagag aaaatgtcgg aattagtatc aaaaatttta
     4381 gtcccaacta ttttaattga ccatcatttg caaccagaaa aatttgcaaa aataaccatt
     4441 tcttatccag agatttcttc aaccagtgag cttatttttc gactgatttg ccgaatgggc
     4501 gatttttcaa aaatcaattt agcttgtgca gaatgtattt atactggaat gatgaccgat
     4561 acaggcaatt ttacttacaa ttcaaatgat ccggaaattt atattatcat ttcagaatta
     4621 atcaaaatag gaattgataa agacgaaatt tatcgcaaag tatttaatac ctattctgaa
     4681 aatcgtctga aattgttggg ttattgtctt tacaaaaaaa tgaaaatttt ttccacatac
     4741 cgaactgcca ttatttctct ttcacaacaa gaattaattg catttaatta tacaaatgga
     4801 gacgctgaag gatttgtaaa catcccgctt tccattgaag gaattgattt ttccgtcctt
     4861 ttgcgagaag aaaaagataa aattaaaatt tcactgcgtt cacaaggaaa ttttccagcc
     4921 aataaggtag ctgccgactt gttcggaggt ggtggtcatt tgaatgcagc aggagccgaa
     4981 agttatacct ctttagaaga aactgaaaaa caattattga acgctttgcc tgattatttg
     5041 atattttttg attgatattt tttgttttta tgaacaagaa aggttttaat actgtttttc
     5101 atagaataac tttatagaaa taaaaacaaa acaaactata taaaaacctc tgtaataact
     5161 attgattatc gagccttatt ataaaatgta tgaaatatgt tgttatttta ggagatggaa
     5221 tggctgatga gcctattgat tcgttaggga ataaaacgcc tttacaagtt gctaataaac
     5281 ctacaataga taaaattgct gctttaggaa gaagtggaat gctaaatact atccccgaag
     5341 gattttctcc gggaagcgaa attgcaaact taagtgtatt aggttatgat gtggctgacg
     5401 tttttgaagg gcgtggttca ctagaagctg cgagtatggg tgtttctctt gaaaaaggag
     5461 aaatggcaat gagatgcaat ttgatttgta tcgaaaatga aaaaatcaaa aaccattctg
     5521 ctggacatat cagcaatgaa gaagctgctg aattaattga atttctcaat aataagcttg
     5581 gaaatgaaaa cattagtttt tatcatggag tttcgtatcg ccatctgtta aaaatgagag
     5641 gaggaaacaa gaatctaaaa tgtacgccac cgcatgatgt gccaggtatt ccctttcgaa
     5701 aagtaatgat aaaagctttg tcccccgatg cacaagctac agcagattat ctcaatgaac
     5761 ttacgcttcg ttctcaggaa ttgcttgaaa atcatcccgt caatttaaaa cgagctgctg
     5821 aaggaaaaga caaagccaac agcatctggc tctggtcgcc aggctataaa ccagaaatga
     5881 agactttatc agaactttat cattttaaaa aaggttctgt catttctgct gttgatctta
     5941 ttaaaggaat tggggtatat gctggtttaa atgttatcaa tgttgaaggt gctacaggtt
     6001 tatataatac caattatgaa ggtaaagccc aagcagctat tgaagcatta cgaactgatg
     6061 attttgttta tgttcacgtc gaagcaagcg atgaagctgg tcatgatggt gatgttccac
     6121 taaaaataaa aacaattgaa tatctggatg cgcgaattgt gaaacccatt tttgaagaag
     6181 tttccaaatg gaacgagcct gttactatag ccgttttgcc agatcaccca actccttgtg
     6241 ctctcagaac gcatacatgt gatcctgttc cttttgtcat ttatcatcct ggagaaacac
     6301 ctgatgaagt gacaacttac gatgaatttg ctgctcgaaa aggaagctat ggtttattaa
     6361 aaggaaatga atttatgaaa aacttaatag gaaattccaa cttatagaaa aatcaaggaa
     6421 tttgaaagtt attatgaatt ttaaatcaca aaaacattaa aaaactaaca aaccttcaga
     6481 aggatgagaa aaactttctt atatttgttt cggaggaaaa ttagttaaaa tcagatttat
     6541 ttcataaaat aattttataa cataactttt atatagtaag tacttacaaa ttcttatacg
     6601 accaaaagga aaaaagaaat aatatccaga tgaaatatta cagtaccaac aaaatagtaa
     6661 agaacattac cttacaagaa gcagtggtaa aaggtttggc agatgataag gggcttttta
     6721 tgcctgaaga aataaaaaaa ctgccttctt ctttttttga taccatgatg aacatgtctt
     6781 tacaggaaat ttcttttatc gttgctgacg ccttttttgg tgatgatatt gattctcggg
     6841 cattaaaaaa aattgtggac gacacattaa attttgatat tccacttata cctgttgaag
     6901 accatattta tagtttggaa ttgtttcatg gacctacact ggctttcaaa gatgtaggag
     6961 cacgatttat gtcacggttg ctgagctatt ttattcaaaa agaaggtgaa agaaatgtaa
     7021 atgtactggt agctacttca ggtgacactg gaagtgcagt tgcaaacggt tttttgggca
     7081 tcgagggaat tcatgtgtat gtcctttatc caaaaggttt ggtaagctct atccaagaat
     7141 gtcagtttac cacattaggg gaaaacatta ccgcactcga aattgaagga acatttgatg
     7201 attgtcagcg gctggtaaaa tcagcctttg tcgacccaga actaaatgct tgctttaagt
     7261 tgacttcagc caattccatt aatattgctc gactattgcc acaatctttt tattatttct
     7321 atgcctttgc ccaattaaga gccattcatc caaaatatac tgctgaagag ctggaaatgg
     7381 ttatatgtgt tcccagtgga aattttggca atcttacggc aggacttatt gccaaacgaa
     7441 tgggacttcc cattaagcga tttattgcag ccaataatcg caatgatgtt tttttagagt
     7501 atcttcaaac aggcgaatat catccacgta cttctgtggc taccatcgcc aatgcgatgg
     7561 atgtgggcaa tccaagtaat tttgatcgga tactcgattt gtatggctat tctcacgaag
     7621 ctattaccaa agatataagc ggaatcagtt atactgatga gcaaatcgct gcaacgatca
     7681 aaaactgttt ctctaaaaat ggttatttac tcgatcctca tggagcttgt ggctatcaag
     7741 ccttgaaaga caaattatta cctgaagaaa atggtatatt tttggaaaca gctcatcctg
     7801 caaagtttct tgatacggta gaaaaaatta ttggtgaaaa aattgaaatc cctgcaaaac
     7861 ttcaggaatt catgaaagga gaaaagaaaa gtattccgtt aagtaaagat tttgaagaat
     7921 ttaaaaagtt cttattggca aatgctcatt gattttttca gaaaatgaca aataaatatt
     7981 ttttaaacta caacaaaaat aatgtatcac caccctcctc aatttatcaa agaatttacc
     8041 agaaaacaaa tccattatcc aaacctgtaa aatcatacca tacctgtaag cactaaaaga
     8101 aaataactat cctgaccagc aataaagtca aaaataacct gcttcaacaa atatcctttt
     8161 ctatccgaaa tcaatcaaca atttcgaaaa aacaaatgct atatttaata aacaaaaaac
     8221 aataaatagc acactaaaaa atttgttttc cttcaaggta aaagcatgtg aaaataacaa
     8281 agaagaaata taagcaaaaa aaggaaggta aaatattctt tgataaaaat tcaacaaaga
     8341 aagaacgata aataataata aagagaacac aagaaaattc aatctttttc gagtagggat
     8401 agcatttttt tgaaaatcat tataaatgct gccaattatt accaaaagca acaaagcaaa
     8461 taaacctcca taaatacttt ctatactcat aataggttta aaagaaaaat tactttcaaa
     8521 atgaaaatat tgaacaaaca agggatacaa atcgacttga ggttgaaaaa caaaataaaa
     8581 accaaaataa agtacaaaag gggttaacca gcccaataaa gatgccagaa aagtttttaa
     8641 ggtaaaattt tgaaactgaa aaaaacctac ccagaataga ggaataataa atattaaaga
     8701 aggcaaaaat aaactacaaa gtcctattaa taaactacca atataggctt gctcaactgg
     8761 ttggttttga tgaaacatat cgaacaaaaa aaagaaagaa ccaatgaaaa aagttagtac
     8821 tatttgcagg atccacatcg aatgaatgcc ataccatgtt gacatcaaca tcaagaaaat
     8881 aaaaaatggt aagaaggttc ttgcatgaat aatggtgaaa cgattgttta aatgttcagc
     8941 caacatccca ttaaaaatag tcaatacaaa acttaaaata ttagcgatta caatattaac
     9001 tggaagattt tcctctaagg ttgccatcaa aggggaaaga gaagacaaag aaggaggatt
     9061 tgttttaata aaaaaagaaa ttatccacaa tcccaagcaa agtaaaatca aaaaaccacc
     9121 taacggaata gtaggaacaa taattttctt aatggaaatg ttgttttttt tcataaaagg
     9181 attcaactat cagtgcattt gataaaacaa atttcataat aaaattagat aattcaatga
     9241 tata
//
LOCUS       DAOSAS010000070         9023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_5051_length_9023_cov_13.978368, whole genome shotgun sequence.
ACCESSION   DAOSAS010000070 DAOSAS010000000
VERSION     DAOSAS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5051_length_9023_cov_13.978368"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..2253
                     /locus_tag="PLR72_04500"
     CDS             154..2253
                     /locus_tag="PLR72_04500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOK36497.1"
                     /translation="MKKSSFILLLIMSMLLSNCNMDKNSLLQNYDTPYGVPPFDRIKT
                     EHYLPAFHAGIKQHQDEIDKIATDKSAPTFDNTIAALDYSGELLKKVSYVFFNLYSTD
                     SNEQMEAIAEQITPQLAEHNDNLYLNEALFKRIKTLYEQKDKLGLSFEQNRLLEKYYK
                     NFVRNGAALNAEQKAQLREINKELSLAELQFGQNVLAETNAYQKWITDEKDLAGLPDN
                     VRQAAAEAAEQAGRKGAWLFTLQKASFIPVLQYSKNRDLRKELVTAYTHIANNNNQHD
                     NKAVINKIMKLRIQKAQLLGFPTSADYYLDDTMAKTPDKVYSFLASLWEPSLKKAKEE
                     AAELQQLMDTEGKGEKLEPWDWWFYAEKLRKIKYDLNEEVLRPYFKLENVRQGVFDLA
                     SRLYGLQFKKLNNMPVYHPEVEVFEVLDADGSLVGILYTDYFPRAGKQSGAWMNNISE
                     QYIKNGVNFRPVICNVGNLTKSIAEHPSLLNMDEVETIFHEFGHALHGLLAQSTYPSL
                     SGTNVVRDFVEFPSQIMENWCWEPEVLKTYARHYQTNEIIPDDLIEKIQQSRLFNQGF
                     NMTELLAAAYLDMDYHTRKDTSDFNVEDFENASMAKIGMIPQIHVRYHSTYFNHIFTG
                     GYCAGYYSYSWAEVLADDAFQAFKETGDVYNQELSFAFRKKILERGDSDEPMKLYIDF
                     RGKEPLPDALLIKRGLK"
     gene            2785..5721
                     /locus_tag="PLR72_04505"
     CDS             2785..5721
                     /locus_tag="PLR72_04505"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HOK36498.1"
                     /translation="MKIYCCCCLKQLLNNKFFIIISFLIFPQWYLMAIHPCVRNFSHK
                     TTHSGTQNWDIIQYRNNWMYFANNNGLLEFDGNKWTVYPISNYTNVRSLYYDIKTDRI
                     YAGAFNEFGYYSRDEAGLLHYYSLIEKIPAKNRNFNEIWNISAVDNSIYFQGDKDIFQ
                     YKNDQISHFSFPAKIDCSAVVFNSLIVCSKQNGTFFLNGDMFIKIPNSELLTNKRVSA
                     ILPFQKNNVLFVTEFNGLFTFDGNKIEPFKTDIDQFMSENQVFCAAIKDYKLAIGTVR
                     KGLVIKDFQENSNFFSNTFSGLQNNTILSLAFDNQDNLWLGLDKGIDYVLINSPIYEL
                     ISSINQIGTGYCSLIENENLYLGTNQGLYCTLYPIPVSPDPPPIQLINGMQGQVWCLM
                     KIDGTIFCGTDHGAYYFSGNKIVQIPGIPGTWSFRKMNQHPDCVLGSSYQGFFILKKN
                     KQKWEFSNFIKGFTDSGGMFEMDENDNIWFSHWIKGISKLTLNQTLDSVIHIETYDTT
                     KGLPTNRNNTLLLYKNSIKFSSEHGFYKYNKKKDCIEEDEEFNNLFNYPPHSVHLFEN
                     NRGDIWCISTTFIAVALKQENQKYKVDSISFNALKSMIVPGFEHFNFVDNDHLIVSTE
                     DGFSWININNINNKNTSNHFQVSISNVYLTSKFDSLANGYIATQNLKHLPKYDHKHNS
                     IRFEFTAPEFRKEGMVTYSYKLENYDSEWSTFSPNTSKEYTKIKKGTYTFRVRAKSQL
                     VPQIAETSYQFIILPAWYETTTAYIIYSLIFVLLVVLLLNFIKKSSEKGAMKMKEQKE
                     HEMKEQEKKFLLEAKEKEKEIIALKNQRLQYELRHKSQELADSTMNLIRKNEILTDII
                     QNLNKISSSLNKDKEHQTIFNELKKIQEEIKSNIEHDDNWKKFEENFDLVYENYLKRL
                     TETYPSLTITDKKICAYLKMGLSSKDIAPLLNTSYRSVEMSRYRLRKKMNLDRNVSLS
                     DFLQKF"
     gene            complement(6059..7243)
                     /locus_tag="PLR72_04510"
     CDS             complement(6059..7243)
                     /locus_tag="PLR72_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOK36499.1"
                     /translation="MKKIKSIIFIMAIFSMNIFAQTSNSFQQVSSAINDYRLVWQDNF
                     DGTQLNSNNWVIEVNGEGGGNNELQYYRRENVSVGIEPVSGANCLILTAKKENYSGKT
                     CTSGRVNTLGKMSFKHGKIEARIKLPHTANGLWPAFWMMGADWPSVGWPKCGEIDILE
                     MGNSEGIRNGTQDRYFNGACHWGETWQNHPMYARATTNPYSLQDDFHLFTLVWDENVI
                     KMYLDLDKYPSNNPYYEMNISQNSDPSHPCHYFHKPFFVIFNLAIGGDFTGIRDINNV
                     TALNAGNNYQAKMYVDYVKVYQKGDTSEEYHGPTMGINKTTLLNKFNIIPNPFFDQIT
                     ISGEKVPSLVTVYNVTGKMILQVSNSNIIQTSNLPEGIYFIKIKDSNGRVENHKIIKE
                     NK"
     assembly_gap    7314..7413
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7414..>9023)
                     /locus_tag="PLR72_04515"
     CDS             complement(<7414..>9023)
                     /locus_tag="PLR72_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK36500.1"
                     /translation="DPVTKFIVNDETNGITDKVIVADASGNATLSGLTPETTYHLIIK
                     AKDAAGNVSENYKTVEFTTGVLIGNYCQTTITATDNVTTVRLSCQMISDGNYQIKIES
                     DVSMTNLSPGCYCYINGNPGNQLISLPGYVRSSDGKTITIDIPSTSAPNLYTPLFILF
                     EQEGGGEIEKTFDWPSDIIWGQCDTEAPTSFTADVGIVTSTSVELLLYAVDNRSVIVY
                     TVSYGSEPTIVTQNGTSGTQKSFVITGLIPETTYTFSIEAKDQFNNEASNSPIELEVT
                     TTNGIPSTALTPAHIWPAGKVISIYSDVYTSVTGLNLNPDWGQSTSQSEIQVNGNNIL
                     KYENLDYQGIEFDHIHPISSKMNHLHIDIWTENETFLQLYPICWDAEESANEPEKYIT
                     LFPLNIGEWNSFDIPLTDFTSQGLTMEDVYQIKIVGSGNTPGETKKTVYIDNLYFYND
                     LSNSFSNLSVDEVICYPNPVKDLFVVKAKSEIGQIIIRNMKGQSIKNISVNGLEKKVD
                     VTNLPAGDYTFTVKLLNGHVFNGKLVKQ"
BASE COUNT         3106 a         1469 c         1409 g         2939 t
ORIGIN      
        1 ttaaaaacca acttacaaag taacattcgt gcttagaagc acggtacggt gagcctttaa
       61 aattttgtgg ccttgatttt tattgtgcgg cgtataaaaa tcattttttt atataagttt
      121 gcatatttaa tttctttcaa aaaaatatta aatatgaaaa aatcatcttt tatattatta
      181 ttgataatga gcatgttact tagtaattgc aatatggata aaaattcttt attacaaaat
      241 tatgatactc cttatggagt gccgcctttc gatcggataa aaactgaaca ttatcttcct
      301 gcttttcatg ctggaataaa gcagcatcag gacgaaattg ataaaattgc taccgacaaa
      361 tcagcaccta cttttgataa tacaatagct gcactagatt atagtggcga attgcttaaa
      421 aaagtttcgt atgtcttctt taatctttat tccactgatt caaatgagca aatggaagcc
      481 attgctgaac aaatcacacc acaactcgca gaacataacg ataatttata tctcaatgaa
      541 gcacttttca aaagaattaa aacgctttat gaacaaaagg acaagttggg tttgagtttt
      601 gaacaaaatc gtttgctgga aaaatattac aagaattttg ttcggaatgg agctgcttta
      661 aatgcggaac aaaaagctca acttcgggag attaacaaag aattatcgct cgctgaattg
      721 caattcggtc aaaatgtatt agcagaaacc aatgcctatc aaaaatggat taccgatgaa
      781 aaagatttag caggtttgcc agacaatgtt cgtcaggcgg cagctgaagc tgcagaacaa
      841 gctggtagga aaggtgcctg gctttttact cttcagaaag caagttttat tcccgttcta
      901 cagtacagta aaaatcgtga tttacgaaaa gaattggtaa cagcttatac ccatattgcc
      961 aacaataata atcagcatga taataaagca gttattaaca agattatgaa acttcgcatc
     1021 cagaaagctc aattactggg cttcccaact tcagctgatt attatctgga cgacacaatg
     1081 gcaaaaacgc ccgataaagt ttacagtttc ctggcttcgc tttgggaacc ttcattgaaa
     1141 aaagcaaaag aagaagccgc tgaattgcaa caactgatgg atactgaagg aaaaggagaa
     1201 aaactggaac cgtgggattg gtggttttac gcagaaaagc ttcgcaaaat taagtatgat
     1261 ctgaacgaag aagttttgcg accttatttt aaactggaaa atgtacgtca aggtgttttt
     1321 gatctggctt cacgattgta tggcttgcaa tttaaaaaat taaataacat gcctgtttat
     1381 catccagaag ttgaagtgtt cgaagtgctg gatgcagatg gttctttggt gggaatttta
     1441 tatactgatt attttcctcg tgcgggcaaa caatcgggag cctggatgaa taatatctcc
     1501 gagcagtata tcaaaaatgg cgtcaatttc cgaccagtga tttgcaatgt gggaaatcta
     1561 accaaatcta tagcagaaca tccttccttg ctcaatatgg acgaagtgga aacaattttt
     1621 cacgaatttg gtcatgcatt gcatggattg ctggcacaaa gcacttatcc cagtttatca
     1681 ggaaccaatg tagtccgtga tttcgtagaa tttccttcac aaatcatgga aaactggtgc
     1741 tgggaacctg aagtattgaa aacgtacgct cgtcactatc aaaccaacga aataattccc
     1801 gatgatttga ttgaaaaaat tcagcaatca cgattgttca atcaaggatt taatatgacg
     1861 gaattgctgg ctgctgctta tctggatatg gattatcata ctcgaaaaga taccagtgat
     1921 tttaatgttg aagattttga aaatgcttca atggcaaaaa ttggtatgat tccgcaaatt
     1981 catgtcagat atcacagcac ttattttaat cacattttta ctggtggtta ttgtgctggc
     2041 tattacagct attcctgggc agaggtgctg gctgatgatg cttttcaagc ttttaaggaa
     2101 acaggtgatg tttacaatca agagttatca ttcgctttca ggaaaaagat actggaacgt
     2161 ggcgattcgg atgaaccaat gaaattatat attgattttc gtggcaaaga acctctacct
     2221 gatgcacttt taattaaaag aggattgaaa tagttcatta ttccaaaatt tgttttgtgt
     2281 ttcataccaa attgcttttc aaaagtgttt gtcgaggtac gaggtatagg tttcgagttc
     2341 tgtgttttga atatttacat ggtaatcaag aatttgtagt tttaggtttg gagcaacgcc
     2401 ccattcagct aacccaccca caaaccatct tccacaaaaa caaagatttt ttccagttac
     2461 gagtttcaaa tttgcaagta aaaatctttg aaaaacaatc aacgttttta ggtgtttaca
     2521 tagacaatag taagaaaaaa ctaactttaa aaaattaaga agaaagaaga atctttcagt
     2581 actttttata ataaaaaatc aacttccaat ttttttttca aaaaaaatat taaatttgac
     2641 ttgtaaatac ttaatttttt tactataaaa aatattttct caggttaaaa tggaacataa
     2701 aattaatgac atagaaataa aaagatatca ttcttctatc ctattaattt aatctttcat
     2761 gtataatttc caatttgttt cgatatgaaa atctattgct gttgttgtct aaaacagtta
     2821 ttaaacaaca agttttttat tataatttct tttctgattt ttcctcaatg gtatttaatg
     2881 gcaattcatc cctgtgtccg gaatttttcg cacaagacaa ctcattctgg tactcaaaac
     2941 tgggatatta ttcaatatcg aaacaactgg atgtattttg ccaacaacaa cggattgttg
     3001 gaattcgatg gaaataaatg gaccgtttat cctataagca attacacgaa tgttcgttct
     3061 ctttattatg atataaaaac tgacagaata tatgccggag ctttcaatga atttggttac
     3121 tattcacgtg atgaagcagg actattgcat tattattcat tgatagaaaa aattccagct
     3181 aaaaacagaa actttaacga aatctggaat atttcagccg ttgataattc aatttatttt
     3241 caaggagata aggatatttt tcaatataaa aatgatcaaa tttcacattt ctctttccct
     3301 gctaaaatag attgttcagc agtcgttttt aattctttga ttgtttgcag taaacaaaat
     3361 ggaacatttt tcttaaatgg cgatatgttt ataaaaattc ctaattctga acttttaact
     3421 aacaaaagag tcagtgctat tttacctttc cagaaaaaca atgtcctatt tgtaactgaa
     3481 ttcaatggat tatttacttt tgatgggaac aaaattgaac ctttcaaaac agacattgac
     3541 caattcatgt cggaaaatca agttttttgt gctgcaataa aggattataa gctggcaatc
     3601 ggtaccgtaa gaaagggatt agttattaaa gattttcaag agaattccaa ttttttttca
     3661 aatactttca gtggattgca aaataatacc attttaagtt tagcgtttga caatcaagat
     3721 aatttatggc ttggacttga taaaggcatt gattatgttc ttattaattc tcccatttac
     3781 gaattaattt cgagtattaa tcaaatcggt actggttatt gttctctgat agaaaatgaa
     3841 aatttatacc ttggcactaa tcaaggcttg tactgcactt tatatccaat acctgtctcg
     3901 ccagatccac ctcctattca actgataaat ggtatgcaag ggcaagtatg gtgtttaatg
     3961 aaaatcgatg ggaccatctt ttgtggaaca gaccacggag cttattattt ttcaggcaac
     4021 aaaattgtac agattccagg gataccaggt acttggagtt tccgaaaaat gaaccaacat
     4081 cctgattgcg tacttggatc ttcttatcaa ggttttttta ttctcaaaaa gaacaaacaa
     4141 aaatgggaat tttcaaattt cattaaaggt tttacggatt caggaggaat gtttgaaatg
     4201 gacgaaaatg acaacatatg gttcagtcac tggatcaagg gtatttcaaa actaacgttg
     4261 aatcaaactt tagatagcgt catacatatt gaaacttatg atacgacaaa aggtttgcct
     4321 accaatagga ataacacctt attattgtat aaaaattcaa ttaaattttc aagcgagcat
     4381 ggattttata aatataacaa gaaaaaagat tgcattgagg aagatgaaga atttaacaat
     4441 ctttttaact atccgcccca ttctgttcat ctctttgaaa ataatagagg agatatttgg
     4501 tgcatttcaa caacttttat agctgttgcc ttgaaacaag agaatcaaaa atataaagtt
     4561 gactccattt cattcaatgc tttaaaatca atgattgttc caggttttga acatttcaat
     4621 ttcgttgaca acgatcattt gattgtcagt actgaagatg gtttttcatg gattaatatt
     4681 aacaacatta ataataaaaa tacaagcaat catttccagg tttccatcag caatgtttat
     4741 ttaacgagta aatttgattc acttgcaaat ggctatatag caacccaaaa cttaaaacac
     4801 ttaccaaaat atgatcataa acataattct attcgttttg aatttacagc tcctgaattt
     4861 agaaaagaag gaatggttac ttacagttat aaacttgaaa attatgattc agaatggtca
     4921 actttttctc caaatacttc aaaagaatat acaaaaatta aaaagggcac ttacactttt
     4981 cgagtgaggg ctaaaagtca acttgttccc cagatagcag aaacttctta tcaatttatt
     5041 attttgcctg catggtacga aactacaaca gcttatatta tttatagttt gatatttgta
     5101 ttgttagtgg ttttactgct caattttatt aaaaagagtt cagaaaaagg agcaatgaaa
     5161 atgaaagaac aaaaagaaca tgaaatgaaa gagcaggaga aaaaatttct cctggaagcc
     5221 aaagaaaaag aaaaagaaat tattgccttg aaaaaccaac gacttcaata tgaattaaga
     5281 cataaatcac aagaacttgc cgattccacc atgaatttga tacgaaaaaa tgaaatacta
     5341 accgatatta ttcaaaactt aaataaaata tcttcctcct tgaacaaaga caaagaacat
     5401 cagacaattt tcaatgaatt aaagaaaata caggaagaaa ttaagtcgaa tattgagcac
     5461 gatgacaact ggaaaaaatt tgaggaaaat ttcgatttgg tctatgaaaa ttatttgaaa
     5521 agactcactg aaacttatcc aagtttaacg atcaccgaca aaaaaatttg tgcttatctc
     5581 aaaatgggtt taagttccaa agatattgca cccttgttaa atacgtcata tcgaagtgtg
     5641 gaaatgagca ggtatcgctt gcgaaaaaaa atgaatcttg atagaaatgt aagtttatcg
     5701 gatttcctcc aaaaattctg atactcttta taaaacggat tttcctttcc aaattttgta
     5761 aaaaatgatc taaaagccaa tatcaatcgg cttttaagaa tattccagta aatttaatgt
     5821 acgcaagaaa aatactattt ttttcttcta acaaagttat tttattgaat ttttttgtat
     5881 gtttgtaaaa aaataaaata atgttcaatt tatgataaac aactaataat tacactgtaa
     5941 gtaagaaatt atttttaaaa aatatattaa acaaataata atcaatttgt tatataaaaa
     6001 tataccattt tttgaaagga aaatccgata agaaatttaa gatacaaaga aagaagaatt
     6061 atttattttc cttgattatt ttatggttct ctacccttcc attgctgtct ttaattttta
     6121 taaaatatat tccttcaggc aaattggaag tttgaataat attagaattt gaaacttgta
     6181 aaatcatttt ccctgtaaca ttatatactg ttacaagaga aggaaccttt tctccggaaa
     6241 ttgtaatctg atcaaaaaaa ggattaggta taatattaaa tttatttaat aaagtagttt
     6301 tatttatacc catcgttggg ccatgatatt cttctgaagt atcacctttt tgatatactt
     6361 ttacataatc aacgtacatt ttagcttgat aattattacc tgcatttaaa gccgtaacat
     6421 tattaatatc ccggattcct gtaaaatctc caccaatcgc taaattaaaa attacaaaga
     6481 aaggcttgtg aaaataatga cagggatgag agggatcaga attttgtgaa atattcattt
     6541 cataataggg attattgctt ggatacttat ctaaatcaag atacatttta attacattct
     6601 catcccacac caaagtaaaa agatgaaaat catcttgaag gctataagga ttggtagtag
     6661 ctctagcata cattggatgg ttctgccatg tttcccccca atgacaggca ccattaaaat
     6721 agcgatcttg cgtcccattt ctaattcctt cactgttacc catttccaaa atatcaattt
     6781 cgccgcattt tggccatcca accgacggcc aatcagctcc catcatccag aaagcaggcc
     6841 aaagtccatt ggcagtatga ggcaacttta ttcgtgcttc aattttacca tgtttaaaag
     6901 acattttacc taatgtatta acacgaccag aagtacaagt ttttcccgaa taattttctt
     6961 tttttgctgt taatattaaa caattagctc cacttactgg ctcaattcca acggaaacat
     7021 tttctctacg gtaatattgc aattcattat tgcctcctcc ctcaccattt acttcaatca
     7081 cccaattgtt actatttaac tgagtgccat caaaattatc ctgccatact aatcgataat
     7141 cgtttatggc tgatgaaacc tgttggaaag aatttgaagt ttgagcaaaa atattcattg
     7201 aaaaaattgc cataataaaa atgatacttt taattttttt cataataact ttatttttaa
     7261 aatttatact gtattaaatc tttactattt aaaattatag attacaattc taannnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntattgtt ttactaattt accattaaat
     7441 acatgtccat tcaataattt gacagtaaag gtataatctc ccgcaggaag attagttaca
     7501 tccacttttt tctctaagcc atttactgaa atatttttta tactttgccc tttcatattg
     7561 cgaataatta tctgccctat ttcagatttt gctttaacaa caaataagtc tttcacagga
     7621 tttggatagc aaatcacttc atcaacagaa agattggaaa atgaattact taaatcatta
     7681 taaaaataca gattgtcaat gtaaacagtc ttttttgttt ctcctggcgt atttcccgat
     7741 ccaactattt tgatctgata aacatcctcc attgttaatc cctgactggt aaaatctgtt
     7801 aaaggaatat caaaactatt ccattctcca atatttaagg gaaaaagagt tatatatttt
     7861 tcaggctcat tagcactttc ttctgcatcc cagcagatag gatataactg caagaatgtt
     7921 tcgttttctg tccaaatatc aatatgcaaa tgattcattt tactacttat tggatgtata
     7981 tgatcaaact ctatcccctg ataatcaagg ttttcatatt taagaatatt gtttccattt
     8041 acttgaattt cagattggct ggtcgattgc ccccaatcag gatttaaatt taatcctgtt
     8101 actgatgtat aaacatcact atatatagag ataactttcc ctgccggcca gatgtgtgca
     8161 ggagtaagag cagtggaagg aatcccatta gtagttgtta cttcaagttc tattggacta
     8221 ttagaagcct cattattaaa ctgatctttt gcttcaatag aaaaagtata agtagtttcc
     8281 ggaattaatc cagttattac aaatgatttt tgggtaccgg aagttccgtt ttgcgtaact
     8341 attgttggtt cacttccata ggagactgta taaacaatca cactcctatt atcaacagca
     8401 tacaaaagga gttctacgct ggttgaagtc acaataccta cgtcagcagt aaaagaagta
     8461 ggtgcttcag tgtcacattg tccccaaatt atatccgacg gccaatcaaa ggttttctca
     8521 atttccccgc ctccttcttg ctcaaataaa ataaataacg gtgtataaag attaggcgca
     8581 gaagttgagg gaatatcaat agtaattgtc ttaccatctg aacttctcac atatcctggt
     8641 aaggaaatta attgattacc tggattacca ttaatataac agtagcaacc aggggaaaga
     8701 ttcgtcattg aaacatcaga ctcaatttta atctgataat tgccatcaga aatcatttga
     8761 caggataacc tgacagtcgt tacattatct gtagcggtaa tagtagtttg acaataattc
     8821 cctataagaa cacctgtagt aaattccaca gttttataat tttccgaaac attgccagct
     8881 gcatcctttg ccttaatgat gagatgatag gtagtttcag gggttaaacc actaagcgtt
     8941 gcattacccg aggcgtcagc tacaatcact ttgtccgtta taccattggt ttcatcgttt
     9001 acaataaact tcgttacagg atc
//
LOCUS       DAOSAS010000071         8963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_5092_length_8963_cov_12.749214, whole genome shotgun sequence.
ACCESSION   DAOSAS010000071 DAOSAS010000000
VERSION     DAOSAS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5092_length_8963_cov_12.749214"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLR72_04520"
     CDS             <1..536
                     /locus_tag="PLR72_04520"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOK36501.1"
                     /translation="IFNKEYQPIINPYANGNAWFVQKIHLAPNADEEMRLLGNIDTKT
                     ELVADQSYASILPTAIQTDSTARIELIHYAPNHLIYSFNSNTDQVAIFSEIYYDKGWN
                     AYINGQKVPYIRANYLLRAMPLKAGMYNIDFKFEPRSYAVGNVIALISSIILILCIAS
                     FIFYSVKIEKQTEKSKS"
     gene            596..1474
                     /locus_tag="PLR72_04525"
     CDS             596..1474
                     /locus_tag="PLR72_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOK36502.1"
                     /translation="MSRKKNNSVSHFKNVHFTSIFSMSLVLFLVGLVCLLLFTARDLS
                     IRVRENINLSIILDNGATNSEIVRIKNYLNAAPFAKSVKYISKDDALKELIESLGEDP
                     QEFLGYNPLLASFEVKLHANYANNDSVAKIESKLKTFPHINRVVYQKDVVDLVNENIK
                     KLSYILLGLTVILLLISLALINSTIRLLIYSNRFLINTMKLVGATSWFIRWPYVRRSI
                     VNGIIAAFLALIYLAGTIAYVQYEFGFKGMFIPAVTAVSVTAIILFLGILLSGVSSYF
                     AVGRYLRMPTDDMYFV"
     gene            1578..3479
                     /locus_tag="PLR72_04530"
     CDS             1578..3479
                     /locus_tag="PLR72_04530"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOK36503.1"
                     /translation="MNHGFIRVAAAVPEVRVADCAFNFSSIANLIQQAEKEYVQVICF
                     PELCLTGYTCGDLFFQKQLLEDAEKSLNDLQLLTASTKAIVIVGMPLRCESRLLNAAV
                     VLQQGKILGIVPKTFLPNYNEFYEKRWFTSAMDIPSTNIFIRGQEIPLGTDLLFSDGN
                     FTFGIEICEDVWSPLPPSTQLCLQGAEIIFNLSASNELVEKHAYRLQLIQQQSARCHA
                     GYVYASAGEGESTTDLVFTGPAIIAENGKILESSKRFTFEPQLIVTEIDVECLQSERM
                     RNNYFHERTTLLTPRKVMIEPTSFPIEELKRMVEPYPFIPSLEKNEESCREIFSIQMA
                     GLAKRWLHTKSQHLLVGISGGLDSTLALLVCVKTADQLHFDRKRIMGITMPGFGTSEQ
                     TYTNAYRLMKSLGITILEIPIKDACLQHFKDIGHDPAVHDTTYENAQARERTQILMDL
                     ANKYQGLVVGTGNLSELALGWTTYNGDHMSMYAVNSGIPKTLVRCLVEWTAKQLDENS
                     KKILKDILQTPVSPELLPAKENGEIAQKTEEVIGPYELHDFFLYYFIRFGFSPNKILF
                     LAKNAFKDQHSEETLRKWLKVFLRRFFSQQYKRSCMPDGPKVGSVNLSPRGDWRMPSD
                     VNFPWEELL"
     gene            3523..4590
                     /locus_tag="PLR72_04535"
     CDS             3523..4590
                     /locus_tag="PLR72_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOK36504.1"
                     /translation="MFLEKTIFGNSLINWGISLLILLGAFLLNKGIVLLNRKVIFKIT
                     GKTKNKLDDILFRSLESPILLAIALLAIWIAFRRLDLSATVDKIFDKAYQFLIVINIT
                     WFIAKLVNALIKEYLLPATKEGSGNKRLDESMIMIIRRLSVGIVWTIGIIMALNNTGV
                     NVGALLASLGIGGLAVALAAQDTLKNFFGGLTIFMDHPFRIGDRIIVNGVDGNVEDVG
                     IRSTKIRTFDRRIVTIPNYKIVDASIENVTIEPMRRVVMKIGLTYDTAPNKMEEALTI
                     LKNMPQKLTEIDDKEVNAYFTDFSDSALIITYIYFIKKKSDIMLTISKVNIEILEQFN
                     AAGLNFAFPTRTIYIENQENG"
     gene            complement(4657..5772)
                     /gene="dprA"
                     /locus_tag="PLR72_04540"
     CDS             complement(4657..5772)
                     /gene="dprA"
                     /locus_tag="PLR72_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOK36505.1"
                     /translation="MNNDTLKYKIALTLIKGIGCNLAKNLLAYVGSAEGVFHEKEKNL
                     AKIPGIGTILSKEIVSQNVLDRAEAEIDFIEKNHIQPLFFTDKEYPFRLKECNDAPIM
                     IYCKGECNLNDGKFLGVVGTRKATEEGKRNCRQLIEELSSALPDLIVVSGLAYGIDIC
                     AHKTALANKTSTIAVLGHGLDRIYPASHRSIAVKILKNGCLLTEYLTNTNPDRQNFVR
                     RNRIIAGLCDAVVVVESAIKGGALITAELANDYDRDVFAFPGRVTDERSAGCNALIKN
                     NKASLIESAEDVLQFMNWDKQNINNQGNIQKTMYFDLSHEEQEILSVLRHSHDGIQVN
                     ELAVSLSQPFSKISSLLLEMEFKGLVVCLPGNLYKAI"
     gene            6310..7098
                     /locus_tag="PLR72_04545"
     CDS             6310..7098
                     /locus_tag="PLR72_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOK36506.1"
                     /translation="MKKSIVFFLLIVLLTSCSKVLITGRKQLLLIPDSEILAMSSESY
                     RQYLDSVPLSTDKTNTALVKKVGKNISTAVENYLRANGLASEITNFSWEFNLVKDPSK
                     NAFCMPGGKVVVYEGILPVTKTEAGLAVVMGHEIAHAVAKHSSERLSQEMLAQFGAQL
                     TDLLITNKSAVTKAGIDALYGLGVQVGVLLPYSRKQEYEADRLGLIFLAMAGYDPNEA
                     ISFWERMSAESTNTIEFLSTHPSDAKRLAKMKEYLPEAMKYYKK"
     assembly_gap    7138..7237
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7263..7622)
                     /gene="folB"
                     /locus_tag="PLR72_04550"
     CDS             complement(7263..7622)
                     /gene="folB"
                     /locus_tag="PLR72_04550"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOK36507.1"
                     /translation="MTKISLENMEFHAFHGCLEHERKFGNLFFVSITMEVDTSLADES
                     DQLVDTIDYQQVYNVVKEEMEKPSKLLEHVAKKIMDHLFLQFPQIESLEVKVSKFFPP
                     LGGKVEKATVQMFKSRQ"
     gene            complement(7659..8888)
                     /gene="xseA"
                     /locus_tag="PLR72_04555"
     CDS             complement(7659..8888)
                     /gene="xseA"
                     /locus_tag="PLR72_04555"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOK36508.1"
                     /translation="MNHITLVELTEQIQETININFDQPVWVEAEISELRENPSGHCYL
                     DLIEKEKNSDVLLARIKANCWAPVYRILKPYFENTTGQPLRSGLKILISVTVDYHPLY
                     GISLTIHDIEPSFTIGELALRQMEIIRQLEEEGIADMNKQLPFPLLPQRLAVISSPTA
                     AGYGDFCDQLKNNPYSFVFHTKLFPAIMQGEQAANSIISALEKIYQYVDLFDLVVIIR
                     GGGATADLSCFDSYELALHCAQFPLPILTGIGHQRDISILDMMAHTSLKTPTAVADFL
                     ISNLQEAENQLKNLISNISFFAKQKVEMGKNTLNITRFQLKQTLKTWLIQKNHELEKE
                     KNRLKNAIHFALAKEQNKLGMYEKIIQTHSPAFLLKYGYTITTLKGQKITSVEQVKKG
                     DCIQTYVIDGNFESEVL"
BASE COUNT         2902 a         1483 c         1560 g         2918 t
ORIGIN      
        1 taattttcaa taaagagtat cagcctatta tcaatcctta tgctaatggc aatgcctggt
       61 ttgtacagaa aattcatttg gcaccgaacg cggatgaaga gatgcgttta ttaggcaata
      121 ttgataccaa aaccgaactg gtagctgatc aatcttatgc ttcgatacta cccactgcta
      181 ttcagacaga tagcacggca agaattgaac tgattcatta tgcacctaat catttgattt
      241 acagttttaa ctccaatact gatcaggtag caatattttc agaaatttac tacgataaag
      301 gttggaatgc ttacatcaat ggtcaaaaag taccgtatat acgggctaat tatttattac
      361 gagccatgcc actcaaagca ggtatgtata acattgattt caagtttgaa ccccgtagct
      421 atgcagtagg gaatgtaatt gctcttattt cttcaataat attgatttta tgtatagcaa
      481 gttttatttt ttattcggta aaaattgaaa aacaaacaga gaaaagcaaa agttgataat
      541 tttagaaact ttctaattta tttatgattt tgattaaaaa aagataaata ataaaatgtc
      601 aagaaagaaa aataattccg tttctcattt taaaaatgtt cattttactt cgatttttag
      661 catgtcgttg gtattatttc tggtaggact ggtgtgtctg ttgcttttta cagctcgtga
      721 cttgagcatt cgggtaagag aaaatattaa tttatctatt attttggaca atggtgccac
      781 caatagtgaa atagtaagga ttaaaaatta tttgaatgcg gctccttttg caaaatccgt
      841 aaaatatatt tcaaaagatg atgctctgaa agaacttatt gaaagtttgg gcgaagatcc
      901 gcaggaattt ttgggttaca accctttgct ggcatcgttc gaagtaaaat tacatgccaa
      961 ttatgccaac aatgacagtg tagcaaaaat tgaatcgaaa ttaaaaactt ttccgcacat
     1021 caaccgtgtg gtttatcaga aagatgtagt ggatctggtc aatgaaaata taaaaaaact
     1081 aagttatatt ttgctggggc tgacagttat tttattactt atttcactgg cgcttatcaa
     1141 cagtaccatt cgtttactga tttattccaa tcgcttttta ataaatacaa tgaaactggt
     1201 aggggcaact tcgtggttta ttcgctggcc ttacgtcaga cgaagcatcg ttaatggaat
     1261 tatagcagct tttttggcat tgatttatct ggctggaaca attgcttatg tgcaatacga
     1321 atttggcttt aaaggaatgt tcattccagc ggttacggct gtttctgtca cagctattat
     1381 tttgtttctg ggtatattgc tcagcggcgt ttcttcttac tttgctgtag gacgttattt
     1441 gcgaatgcct acggatgata tgtattttgt gtaatttttt tagtccttat atttaagaga
     1501 tttttcagca aagtgaatat taatttttta agtatttctt attttttatt ggataaaaat
     1561 taaaaaacaa gctcaatatg aatcatggtt ttatccgcgt ggctgctgct gttcctgaag
     1621 tcagagtggc agattgtgct tttaattttt ccagcattgc taacttaatc cagcaggcag
     1681 aaaaagaata cgtgcaggtt atttgtttcc ctgaattatg tctcactggt tatacttgtg
     1741 gagatctttt ttttcaaaag caattgctgg aagatgcaga aaaatcgttg aatgatttgc
     1801 aattactcac tgcctcaaca aaggcaatag taattgtagg tatgccattg cgttgtgaaa
     1861 gtcgactttt gaatgcagct gtcgtattac agcaagggaa aatactggga atcgtaccaa
     1921 aaactttttt acccaattat aatgagtttt acgaaaaacg ctggtttact tctgcaatgg
     1981 atataccttc tactaatatt tttattcgtg ggcaagaaat tcctttagga actgatcttt
     2041 tattttcgga tggcaacttt actttcggaa ttgaaatttg tgaagatgta tggtcgcctc
     2101 ttccgccaag cacacaattg tgtttgcaag gagctgaaat tattttcaat ctttctgcaa
     2161 gtaacgaact cgttgaaaaa catgcttatc gtcttcaact tattcagcaa caatcagcac
     2221 gctgtcatgc aggttatgtt tacgcttcgg ctggtgaagg tgaatcaact accgatctgg
     2281 tttttacagg acctgccatc atagccgaaa atggaaaaat tttagagagt tctaaacgat
     2341 ttacctttga gccacaactg atagtaacag aaatcgatgt agaatgtttg caaagtgaac
     2401 gaatgcgaaa taactatttt catgaacgaa cgacattgct taccccaaga aaagtaatga
     2461 ttgagccaac ttctttccca atcgaagaac taaaaagaat ggttgaacct tatcctttta
     2521 ttccatcact agaaaaaaac gaggaaagct gtcgggaaat tttttccatt caaatggcag
     2581 gtttggcgaa acgctggctg catacaaaat cacaacattt gctggtagga atttctggcg
     2641 gactggattc tacacttgct ttgttggttt gcgtaaaaac agccgatcaa ttgcattttg
     2701 accgaaaacg cattatgggt ataacgatgc caggatttgg aacttcagaa caaacttata
     2761 ccaacgctta ccgtttaatg aaatctttag gaatcacaat tctggaaatt cccattaaag
     2821 atgcttgttt gcaacatttt aaagatattg gacacgatcc tgctgttcac gatacaactt
     2881 acgaaaatgc tcaagctcgt gaacgtacgc agatattgat ggatttggca aacaaatatc
     2941 aaggtttggt agttggcaca ggaaatttat cagaattagc tttgggttgg actacttaca
     3001 atggcgatca catgtcgatg tatgcagtga atagcggaat cccaaagaca ttagtacgtt
     3061 gtctggttga gtggactgct aagcaactgg acgaaaattc aaaaaaaatt ctgaaagata
     3121 ttcttcaaac acctgtaagt cccgaattac tacccgctaa ggaaaatggt gaaattgctc
     3181 aaaaaaccga agaagttatc ggtccctatg aattacacga tttctttttg tattatttta
     3241 tacgttttgg tttttctccc aataaaattc tttttttagc caaaaatgcc tttaaagatc
     3301 aacattcaga agaaacattg cggaaatggt taaaagtatt tttgcgtcgt tttttttctc
     3361 agcaatataa acgttcttgt atgcccgatg gtcccaaagt aggttctgtt aatctttcac
     3421 cccgaggtga ctggcgaatg cccagtgatg tcaattttcc atgggaggaa cttttatgag
     3481 ttcaaataaa attgatttta aataaaaaaa atttaataaa atatgtttct tgaaaaaaca
     3541 atttttggga attcacttat aaattgggga atttctttgc ttatcctgct aggagctttt
     3601 cttttgaaca agggaattgt attattaaat cgaaaagtaa tttttaaaat tacaggcaag
     3661 acgaaaaata aactggatga tattttattc agatcgctgg aatcccctat tttattggca
     3721 atagctttat tggctatctg gattgctttc cgccgattag atttgagtgc taccgtggac
     3781 aagatttttg ataaagccta tcaatttttg attgtcatca acattacgtg gtttattgca
     3841 aaactggtta atgccctcat aaaagaatat ttgcttcctg ctaccaagga agggtcaggt
     3901 aataagcgtt tagatgagag catgattatg attatacgcc gtctctcggt gggaattgtt
     3961 tggactattg gtatcattat ggcactaaat aataccggtg taaatgtggg tgctcttctt
     4021 gccagcttgg gaattggtgg tttagctgtt gctctagctg cccaggatac tctaaaaaat
     4081 ttttttggtg gattgactat ttttatggat catccttttc gtattgggga tcgtatcatc
     4141 gtaaatggag tagatggtaa tgtagaggat gttggtattc gcagtactaa aatccgaact
     4201 ttcgatcgca ggatcgttac tattcccaac tataaaattg tggacgcttc tattgaaaat
     4261 gttactattg aacctatgcg acgagtagta atgaaaattg gcttaactta tgatactgcc
     4321 cctaataaaa tggaagaagc tcttactatt ttaaaaaata tgcctcaaaa attgacagaa
     4381 atcgatgata aggaagtaaa tgcttatttt acagattttt ccgattccgc cctcattatt
     4441 acctatattt attttattaa gaaaaaaagt gatattatgc ttactatttc gaaggtaaat
     4501 atagaaatat tagaacaatt taatgcagca ggattgaatt ttgctttccc caccagaact
     4561 atttatatcg aaaatcaaga aaacggataa tctatttatc tacattcaaa aacaattttt
     4621 gaacttaaaa attcccgtca aaagaagaat tttccatcaa attgctttat aaagatttcc
     4681 aggaagacaa actaccagcc ctttgaattc catttcaagc aagagtgaag aaattttgct
     4741 aaaaggttgg gacaatgaaa ccgccaattc atttacctga ataccatcat gagaatgtct
     4801 cagcacggaa agaatttctt gttcctcgtg agaaagatca aaatacatgg ttttctgaat
     4861 attaccctga ttatttatat tctgtttatc ccaattcata aactgcaaaa catcttccgc
     4921 tgattcaatc aaggaagcct tattattttt aattaaagca ttgcaaccgg cagaacgttc
     4981 atctgttact cgtccaggaa aagcaaatac atctcgatca taatcgttag caagttcggc
     5041 tgtaataagc gcaccacctt ttatagccga ttccacgaca accactgcat cgcaaagtcc
     5101 tgcaatgatg cgatttcttc gcacaaaatt ttgtcgatca ggatttgtat tagttaaata
     5161 ttctgtcagt aaacaaccgt ttttcaaaat ttttactgct atcgagcggt gcgatgccgg
     5221 ataaattcta tccaaaccgt gacctaaaac ggcaatcgtg gatgttttat ttgctaaggc
     5281 agttttatgt gcacaaatat caatgccata agccaaacca ctcaccacta ttaagtcagg
     5341 aagggcactg gaaagttcct caataagttg tcggcaattt ctttttcctt cttctgtagc
     5401 tttacgagta cctaccacac ctaaaaattt tccgtcgttt aagttgcatt cgcccttgca
     5461 gtaaatcatt ataggagcat cattgcattc tttcagccta aagggatatt ctttatctgt
     5521 aaaaaatagt ggttgaatat gattcttttc aatgaaatct atttcagctt cagctctgtc
     5581 taagacattt tgagagacaa tttctttgga taaaatagta ccaatacctg gaattttagc
     5641 aagatttttt tccttttcgt gaaaaacgcc ttcggcacta cctacataag ccagtagatt
     5701 ttttgccaga ttacatccta ttccttttat taaagtaaga gcaattttat attttagggt
     5761 atcattattc acttttcttg aatttttttt tagttaaaac ataaaatata agaaacagca
     5821 ataagcttat taatatgccc gcgatgtcac agatccaatc caaccattca gcagtacgag
     5881 gacggaaaaa tagattttgc aaaacttcaa tcataccgcc taataaaata ggaaataaaa
     5941 cgcaaataaa tagaaacgaa gtttgtttca attttttttg gttatggata aaatcgtaaa
     6001 ttagaatgaa ggtgaaaaaa aagtacataa aaaaatgaac caatttatca gcatatttga
     6061 aagaaggaag tcctgaaaaa gtagatggtg gagcaaatga caaataaagt ataagaaaaa
     6121 cagctaagat tgatttccaa tattttaaaa acgactgcat tgtttagtgc ttaaatagtt
     6181 ttttaattag ttttaataaa aaatcggaac aaaatcggaa caaaatttga acaaagaaac
     6241 tgaacaatta tctttgcaaa gataaatgat tgaaaagtat attaaaaaaa ttacttaaaa
     6301 attttaacca tgaaaaaaag tattgttttt ttcttgttaa tagtgttact gacttcctgc
     6361 tcaaaagttc ttattactgg tcgaaaacaa ttattgttaa ttccagactc cgagattctg
     6421 gcaatgagca gtgaaagtta caggcaatat cttgattcag tacccctttc cactgataaa
     6481 acgaatactg ctctggtgaa aaaagtaggt aaaaatattt cgactgctgt agaaaattac
     6541 ctaagagcta atggtttggc atcagaaatt accaactttt cgtgggaatt caatttggta
     6601 aaagatcctt ccaaaaatgc tttttgcatg cctggcggta aagtagtagt ttatgaagga
     6661 attttaccgg taacaaaaac agaagctgga ttggctgttg tgatgggaca tgaaattgct
     6721 catgcagtag ctaaacacag tagcgaacga ttaagccagg aaatgctggc acaatttgga
     6781 gcgcaattga cggatttgtt aatcaccaat aaatcagcag taacaaaagc tggtatagat
     6841 gctttatatg gtttaggagt gcaggtggga gttcttttgc cttactcaag aaaacaggaa
     6901 tatgaagccg atcgtttagg attgattttt ttggcaatgg ctggttatga tcccaacgaa
     6961 gccatttcat tctgggaaag aatgtctgct gaaagcacta atactatcga atttctgagt
     7021 acccacccca gtgatgcgaa aaggttggct aaaatgaaag aatatttacc cgaagccatg
     7081 aagtattaca agaagtaaaa ataaagcgta aaaacagacg aaattggcaa gtttcgannn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt ttacatcaaa caaaacatac
     7261 ttttattggc gacttttaaa catttgtaca gttgcttttt ctactttgcc acccaatggt
     7321 ggaaaaaact ttgaaacttt tacttctaag gattcaattt gtggaaattg caaaaataaa
     7381 tgatccataa tctttttagc aacatgttct aaaagtttgg aaggcttttc catttcttct
     7441 tttacaacgt tatacacctg ttgataatca atcgtatcta ccaattgatc tgattcatca
     7501 gctaatgaag tatctacttc catagttata cttacgaaaa aaagattgcc aaattttcgt
     7561 tcatgctcca aacaaccgtg aaaagcatga aattccatgt tttcgaggga tattttagtc
     7621 atataatttg tttcaaaaat ttatattttt aaataagctt aaagtacttc actttcaaag
     7681 ttcccgtcta ttacataagt ttgaatacaa tctccctttt ttacctgttc tactgaagtt
     7741 attttctgtc ccttcaaagt agtaatggta taaccatatt tcagcaaaaa agcaggtgaa
     7801 tgggtttgaa taattttttc atacattccc aatttatttt gttctttagc cagagcaaaa
     7861 tgaattgcat tttttaagcg atttttttct ttttcgagct catgattttt ttgtatcaac
     7921 caagttttta aagtttgctt caactgaaaa cgggtaatat ttaaagtatt tttccccatt
     7981 tcaactttct gtttcgcaaa aaatgagata tttgaaatta aattttttaa ctgattttca
     8041 gcttcctgta aatttgaaat taagaaatct gctacagcag ttggtgtctt taaactagta
     8101 tgagccatca tgtctaaaat tgaaatatcg cgttgatgac caatccctgt taaaataggc
     8161 aaaggaaatt gagcacaatg aagagcaagc tcataagaat caaagcaact taaatcagcc
     8221 gttgcaccgc ctcctctgat aatcactacc aaatcaaaca aatcaacata ttgataaatt
     8281 ttttccagag ccgaaataat ggaattagca gcttgttcac cctgcataat agcaggaaat
     8341 aatttagtat gaaaaacgaa tgaataagga ttattcttta attggtcaca aaaatcacca
     8401 tagccagcag cagttggcga agaaatcact gccaaacgtt gcgggagcag tggaaacggt
     8461 aactgtttat tcatatcagc tattccttct tcttctaatt gacggataat ctccatttgc
     8521 ctcaatgcca attcgcctat agtaaacgaa ggctcaatat catggatagt taaactgata
     8581 ccataaagtg gatgataatc aacagttact gagattaaaa tttttagtcc cgaacgaagt
     8641 ggttgccctg tcgtattttc aaaataaggt tttaaaatac ggtaaacagg tgcccagcag
     8701 ttagctttta tcctcgctaa aagaacatca gaattttttt ctttttcaat caaatctaaa
     8761 taacaatgtc cacttgggtt ttctcgcagt tcgcttattt ctgcttcaac ccaaacaggt
     8821 tgatcaaaat ttatatttat cgtttcctga atttgctcgg ttaattcaac aagggtaata
     8881 tgattcattg ttttaactaa atgtaaagtt ttataaagat gttgataggc aatactcaat
     8941 tgctatcata catatatttt aat
//
LOCUS       DAOSAS010000072         8857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_5161_length_8857_cov_12.768348, whole genome shotgun sequence.
ACCESSION   DAOSAS010000072 DAOSAS010000000
VERSION     DAOSAS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5161_length_8857_cov_12.768348"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            529..3741
                     /locus_tag="PLR72_04560"
     CDS             529..3741
                     /locus_tag="PLR72_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK36509.1"
                     /translation="MKKLLLLFFSLFIILSVFSQNIQVQGIVVGQDNEPLPGVNVVVK
                     NTTNGTITNKEGQFTLNVPSNGILVVSYVGYKTQEVPVNGRNFIRIVLEESSEALEEL
                     VVIGYGVQKKSVVTAAISKVTSDELNVVKPSRVEDVLKGKISGVQITQSSGQPGSDSK
                     VRIRGIGTVNNSEPLYIVDGMPVDGGINYLNPVDIQSVEVLKDAASAAIYGARAANGV
                     ILVTTKTGEGVLKGVSKSVINYDFNYGWQNPWKKKSVLDAKEYMVIMNEALINDGNLP
                     RYTASQIQSAGKGTDWQEETFNFDAPVQTHQLSMSGNSEKASYFLSMGYFDQDGIIGG
                     NYGKSNYERWSLRTNATYNVFEDKTRDILNKFRVGVNVGYSRDNSTSIEANSEYGSVL
                     GSALAFNPRVPVYAPETSDDPDIQSVSAILAAHPYAVTDKNGRVFSIPPSGFQEIANP
                     VGMLNQPRSDRWNSDKFVGTFWGELDVLPGLKFKSSYGFDLAFWGNDGYEYPYFLATQ
                     GKDVSQSSVWSQMNRGFKWQVENVLTYNKVFAQLHNLTFVLGQSAQKYTYRQVGGSDY
                     DLLGNDPTLATINAATADRDKERIYGGTGGYTFTSLASYFGRLDYNFAERYMIQATVR
                     RDGSSRFGPNNKWAVFPAVSVGWNVLNEPFLTDNRPNWFDEFKLRFSWGKNGNENIGN
                     FRYTSLMAGGQNYYFGGGYSVATASKTGTMQYGTSPSALPNPNVKWEESEQTNIGFDS
                     RFIHSALSFSFDYFIKKTNGMLMDQPIPGYVGQSAPIGNVGDMKNWGLEFELGWKKSA
                     GDFKYNILANASYLRNELIKLGNASGEAIYETAGASGVGSFIKGKNGEVWPFFYGYKT
                     DGIFQNQAEINSYVNSKGELYQPKAKPGDVRFVDFNGDGVIDDNDKTKIGKGMPDWTF
                     GLTLGAEWKGFDLSLFFQGTEGNDMFDFAMRGDIPAMNRPSWILERWHGEGTSNKIPR
                     MTSADPNSNWRSSDLYIKNGSYVRLKTAQLGYTLPTSLTQKASIHQLRLYVSAENLLT
                     FTGYNGFDPEVASGDYTRIGVDRGVYPQSRTIYVGVNVSF"
     gene            3761..5293
                     /locus_tag="PLR72_04565"
     CDS             3761..5293
                     /locus_tag="PLR72_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOK36510.1"
                     /translation="MKKYKLLSIIPVVLMGILSMTSCSDEFLTARSTEQLEAGGPATE
                     GSILSNLASAYQILLFDSYANYNYNAVLLMSDLRSDDIYKGGGSAGDQEQLYKLSQFT
                     STAAATLDGLWSIYFTGLARCNNVILSCENAQGVNENKLEQYKAEAHFLRAYYVHLLW
                     KFWGNIPYFEEPLSNPPYMAQQLPADSIYNRIMKDIDYACAEGRLAMKTSGSDLGRAS
                     RAAALMLRARVVLYQKDESRYPQITDDMASIITSGEYELFDDFDAMWRDENEFCIESI
                     FESNQLPEGKGWGSAWTGYGTNLPAFISPADLQDPDGVFKGGWGFGPVREEAYNMYDP
                     SDKRRDGSINKWPDGSYTPRFQNTGLFQRKYAARVGYNPPPGDVDLNYCNNLRIFRYA
                     ETLLNYAELVVMHGQSEKQGVVAQDCLDKIRQRAGVGSITANEVNIKSERRKEFLGEG
                     MRFWDLVRWDDAPTVLTETDNEHNSVRTFQDWMKYLPIPQSEIDKTKNTEFELVQNGQ
                     WQ"
     gene            5325..6287
                     /locus_tag="PLR72_04570"
     CDS             5325..6287
                     /locus_tag="PLR72_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOK36511.1"
                     /translation="MKNIIKYGMWIMLSIFMFAACSPQEDEKYSLGELGTITADVISF
                     SQTPSDTSNNVIIFTNTSNLTFPVTAVWDLGNGSTARGNTATGIYPMKGDYTVTLTLY
                     AADGSSASKSEVIHIENDDFGLINTPVYVKLTGGIHDSDGKTWVFDQYNNFAKEVEEA
                     TGFYVKGHMGLAKLNTYSQEWWHAQPNEKKDWKLYDFKFTFIQDGLQLRIQNAGKGYG
                     RSALQSDITSKGFDIIQVADPDIIFSYDGGDYTFSIDESGQYPKLTLSGNAFMGYYCG
                     TQEYEIIYQTDSVMALRVAEPAENLDWIFVYCLEELNVPAIDAE"
     gene            6394..8652
                     /gene="bglX"
                     /locus_tag="PLR72_04575"
     CDS             6394..8652
                     /gene="bglX"
                     /locus_tag="PLR72_04575"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HOK36512.1"
                     /translation="MNVKLFKILLVSFFVFFFLDSTIYGSNLNDKAIEKKVEKLLSKM
                     TLDEKIGQMNQISMFEDSDQLNVLIKEGRLGSIINVANPVEINSLQRVAIEESRLGIP
                     LLIGRDVIHGFKTIFPIPLGQAASFDPNLVKNAARVAAIEASSVGIKWTFAPMIDISR
                     DPRWGRIAEGFGEDPYLTSVMGTAMIKGFQGDILNNFNSIMACPKHFVAYGAAEGGRD
                     YNSTFIPERLLRNVYLPPFEAAVKAGASSIMCSFNDNDGVPSSGNEFILKQVLRKEWG
                     FNGFVVSDWASIKEMINHGFAADEKEAAMKAVNAGVDMEMVSLTYITYLKELIKEGKV
                     KEKTINDAVRNILRIKFRLGLFDHPYVDENQSSVIYAPSHLATAKQLAVESTILLKNA
                     HNILPLTDEVKTIAVIGPMADAPYEQMGTWVFDGDKAYTKTPLNALKEEYGNKINIIY
                     ESALAYTRDKNTEGIAKAVKAAEQADVVLAFVGEESILSGEAHCLADLNLQGAQSQLI
                     EALSSTGKPIITIVMAGRPLTIGKEAALSSAVLYSFHPGTMGGPAIVDLLFGKEVPSG
                     KTPITFPKMVGQIPIYYAHTNTGRPPTGTETMLDQIPVEAGQTSLGNTSFYLDAGFEP
                     LYPFGYGLSYTTFEYSNIKLSSYELKKNDILKISFDLKNTGKYAATEVAQLYIQDKVG
                     SVTRPVKELKRFARVTLQPGEKRTVTFELPISELAFWNIDMKKVVEAGEFNLWVAGDS
                     QSGEKISFKIVD"
BASE COUNT         2863 a         1267 c         1823 g         2904 t
ORIGIN      
        1 agagagaaag gaaaacgatc agcgaagccc tatggaatga gtaggaagga attgcacttc
       61 attattttct gtctttcaaa tcttaaatcg aagtacgaaa ctttagttaa tttaaatctg
      121 cttttaattt gttttttcgg ttgtactata tttttaactt aatcattttt ttatttgatt
      181 ttcaattaga taaatattat gaacaacttg aagcaagtta agaacttatt attctaattc
      241 tgattagaga ttatttaaca tatctccttg tttatttaaa aattcaaatc cgaaagtaaa
      301 ttaatataga attgtaaaat cctttacgac aaaaccaaag attttttagt aaagataata
      361 ttctttatat cagaataata gcatattttg aagtatttaa aacgtatatt ttttttaccg
      421 atgacgtatt tatgtagaaa caaaataagt acattttttt gattttatac ttatatttgt
      481 aatgttcatt aagaacatag tattaatctt aaaattcatt aaaaatttat gaaaaaatta
      541 ttattgttgt tcttttcgct ttttattatc ctttctgtat tttcacagaa tatacaggta
      601 cagggtatag ttgttgggca agataatgag cccttgccag gtgtaaatgt tgtagtaaaa
      661 aatactacaa atggtacgat tactaacaag gaaggacaat ttactctcaa tgttccttcc
      721 aatggcatct tagtggtatc ttatgtgggt tataaaaccc aggaagttcc tgtaaacgga
      781 cgtaatttta tacgcattgt tttagaagaa agttcagagg cacttgaaga attagttgta
      841 attggttatg gtgttcagaa aaaaagtgtc gttactgctg ctattagtaa agttacttcc
      901 gatgaattaa atgtagtaaa accttcacgg gtagaagatg tattaaaggg taaaatctca
      961 ggtgtacaaa ttacgcaaag ttcaggacag ccaggatctg attccaaagt tcgaattcga
     1021 ggcataggga cagttaataa tagtgagcct ctttatatcg ttgatggtat gcctgtggat
     1081 ggtggaatta attatttaaa ccctgtggat attcaatcag tagaagtact taaagatgct
     1141 gcttctgcag caatttatgg tgctcgagct gcaaatggag ttattcttgt taccaccaaa
     1201 acaggtgaag gagttttaaa aggggtttct aaatctgtta ttaattatga ttttaattat
     1261 ggttggcaaa atccatggaa aaagaaatct gtactcgatg ctaaagagta catggtaatt
     1321 atgaatgaag cactgattaa tgatggaaat ttaccacgtt atactgcaag ccagattcaa
     1381 agtgcaggca aaggtacaga ttggcaagag gaaactttca attttgatgc gccagtacaa
     1441 actcatcaac ttagcatgag tggaaattca gaaaaagcat cgtattttct ttctatggga
     1501 tatttcgatc aggatggtat tataggtgga aattacggaa aatcaaacta tgaaagatgg
     1561 agtttgcgaa ccaatgctac ttacaatgta tttgaagaca aaactcgtga tattcttaac
     1621 aagtttagag ttggagttaa tgtaggatat tcaagagata attcaacaag cattgaagct
     1681 aattctgaat atgggtctgt tctggggagt gcattagcat ttaaccctcg tgttcctgtt
     1741 tatgctccag aaacttcaga tgaccctgat attcaatctg tttctgcaat tttggcagcg
     1801 catccctatg ctgttactga caaaaacgga agagtattct caattccacc atctggtttt
     1861 caagaaattg ccaatcctgt tggcatgttg aaccaacctc gatcagaccg atggaattcg
     1921 gataaattcg ttggaacatt ttggggtgaa ttagatgttt tgccgggctt gaaattcaag
     1981 agcagttacg gatttgatct tgctttttgg ggtaacgatg gatatgaata tccttatttt
     2041 ttggctactc agggtaaaga tgtaagtcaa agttctgttt ggagccagat gaatcgaggt
     2101 tttaaatggc aagttgaaaa cgtacttacc tataataagg tttttgctca gttacataat
     2161 ctaacatttg tattagggca atcagctcaa aaatacactt atcgtcaagt aggtggtagt
     2221 gattatgatt tgcttggtaa tgatcctact ttggcaacta ttaatgcagc tacagctgat
     2281 agagataaag aacgtattta tggtgggaca ggaggttata catttacttc attggcttcc
     2341 tattttggac gattagacta taattttgct gaacgttata tgatccaggc aaccgttcga
     2401 cgggatggtt cttcaaggtt tggtccaaac aataaatggg cagttttccc tgcggtatca
     2461 gtaggatgga atgtgttaaa tgaaccattt ttgactgata accgtcctaa ttggtttgat
     2521 gaatttaaac ttcgtttcag ttggggtaaa aatggaaatg aaaatatagg aaattttaga
     2581 tatacctcat tgatggccgg tgggcagaat tattattttg gtggtggtta ctctgtggct
     2641 acggcaagta agacaggaac catgcaatat ggtacttctc cttctgcatt acccaatcca
     2701 aatgttaaat gggaagaatc ggaacaaaca aatattggct ttgattcacg tttcatacat
     2761 agtgccttaa gttttagttt tgactatttt ataaagaaaa ccaatggtat gttgatggat
     2821 caacccatac ctggttatgt tggacaaagt gctcctattg gaaatgtagg agatatgaaa
     2881 aactggggac ttgaatttga attaggttgg aaaaagtcgg caggtgattt taaatacaat
     2941 attttggcta atgcttctta tttaagaaat gaattaatta agcttggtaa tgcatcaggt
     3001 gaagcaatct atgaaactgc aggtgcttcg ggagttggaa gttttattaa aggaaaaaat
     3061 ggagaagtgt ggccattttt ctatggatat aagactgatg gaattttcca aaatcaagca
     3121 gaaattaata gttatgtaaa ttcaaaagga gaactttatc aacctaaagc taaaccagga
     3181 gatgttcgtt ttgttgattt taatggtgat ggtgtaatag atgataatga taaaacaaaa
     3241 attggcaaag gtatgcctga ttggacattt ggtttaacat tgggtgcaga atggaaaggt
     3301 tttgatttaa gtttattctt ccagggaact gaaggtaacg acatgttcga tttcgccatg
     3361 cgcggagata taccggctat gaacagacca tcatggatat tggaacgttg gcatggtgaa
     3421 ggaacttcta ataaaattcc tcgtatgaca agtgcggatc ctaatagtaa ttggcgttct
     3481 tctgatcttt atatcaaaaa tggtagttat gtccgtttaa aaacagctca attaggttat
     3541 accttgccta ccagtttgac acaaaaagca tccattcatc agttacgatt atatgtttct
     3601 gcagaaaact tgttaacctt tacaggatac aatggcttcg atcctgaagt agcttcaggt
     3661 gattatacta gaattggagt tgatcggggt gtttatcctc aatcaagaac catttatgtc
     3721 ggagttaatg tatcattcta atcatttaaa aataaagaat atgaaaaaat ataaattatt
     3781 atctatcata ccagttgttt tgatgggtat actatcaatg acttcatgca gtgatgaatt
     3841 tttaactgct cggtcaactg aacagctgga agctggtgga cctgctactg aaggttccat
     3901 tttatcaaac cttgcatcgg cttaccagat tcttttgttc gatagctatg caaattataa
     3961 ttataatgct gttttgttaa tgtctgattt acgatcagat gacatttata aagggggtgg
     4021 tagtgcaggt gaccaggaac aattatataa actttcacaa tttacttcta ccgctgctgc
     4081 aacacttgat ggtttatgga gtatttattt cacgggattg gctcgctgca ataatgtaat
     4141 tttatcatgc gaaaatgcac aaggagtaaa tgaaaacaaa ttagaacagt ataaagcaga
     4201 agctcatttc cttcgtgctt actatgtgca tttgctctgg aaattctggg gaaatattcc
     4261 ctattttgaa gaaccactaa gtaatcctcc gtatatggca caacaactgc ctgctgattc
     4321 tatttataat cggattatga aagatattga ttatgcgtgt gcagaaggca ggcttgctat
     4381 gaaaacttct ggtagtgatt taggacgagc ttcaagagct gctgcgttaa tgttaagagc
     4441 aagggtggta ttatatcaga aagacgaatc tcgttatcct caaattactg atgatatggc
     4501 ttctattatt acgagtggtg aatatgaatt atttgatgat tttgatgcca tgtggcgcga
     4561 tgaaaatgaa ttctgtatag aatctatttt tgaaagcaat cagcttcctg aaggaaaagg
     4621 ctggggaagt gcgtggacag gatatgggac aaatttacct gcctttattt cacctgctga
     4681 tttgcaagac cctgatggtg tgtttaaagg aggttgggga tttggtccgg tgcgtgaaga
     4741 agcttataat atgtatgacc caagcgataa acgtcgtgat ggttctatca ataaatggcc
     4801 ggatggaagt tatactccaa gatttcaaaa tacaggttta tttcaacgca aatatgctgc
     4861 tcgcgtaggg tataatcctc ctcctggaga tgtagacttg aattattgta acaatttgcg
     4921 aattttccgt tatgctgaaa cattgttgaa ttatgcagaa ttagttgtta tgcatggaca
     4981 gtcagaaaaa caaggggtag tggcacaaga ttgtttagac aaaattcgtc agcgtgcagg
     5041 tgttggttct attactgcaa atgaagtgaa tattaagtct gaaaggcgta aagaattttt
     5101 aggtgaaggt atgcgttttt gggatttggt acgttgggac gatgctccta cagttttaac
     5161 agaaactgat aatgaacata attcggttcg tactttccaa gattggatga aatatttgcc
     5221 aattcctcaa agtgaaattg ataaaaccaa aaatactgaa tttgagttgg tacaaaatgg
     5281 tcaatggcaa taaagtataa atttataatt aaaaagaaaa atttatgaaa aacataatta
     5341 aatatggaat gtggataatg ctgtcaattt ttatgtttgc agcatgtagt ccacaagaag
     5401 atgaaaaata ttcattaggt gaattaggaa caattacagc agatgtaatc tctttttcac
     5461 aaacaccttc agatacgagt aataatgtaa ttatttttac gaatacttcg aatttgactt
     5521 tcccagttac tgctgtttgg gatttaggaa atggttctac agcacgagga aatactgcta
     5581 ctggtattta tccaatgaaa ggagattata ctgttaccct tactctttat gctgctgatg
     5641 gctcttctgc ttcaaaaagt gaagttattc atattgagaa tgatgatttt ggtttaataa
     5701 atacaccggt ttatgtaaaa ttaacaggag gaatacatga ctctgatgga aaaacctggg
     5761 tgtttgatca atataacaac tttgccaaag aagtagaaga agccacgggc ttttatgtta
     5821 aaggacatat gggattggcg aagctcaata cctattccca agaatggtgg catgcacagc
     5881 caaatgaaaa gaaagactgg aaattgtatg attttaaatt tacctttatt caagatggct
     5941 tgcaattaag aattcaaaat gcgggaaaag gatatggtcg ttcagcatta caatctgata
     6001 tcaccagtaa gggatttgat ataattcaag ttgctgatcc tgatattatt ttttcttatg
     6061 atggaggtga ttatactttt tctattgatg aaagtggaca atatccaaaa ttaacacttt
     6121 cagggaatgc atttatgggt tattattgcg gtacgcaaga atatgaaatt atttatcaaa
     6181 cggatagtgt gatggcttta agagttgctg aaccagctga aaatcttgat tggatatttg
     6241 tttattgcct ggaagagttg aatgttccgg ctatcgatgc agagtaatag acaaatacta
     6301 ttgtattttt caacaagagg caggacgttt acgctgcctc ttgttaactg tttttaatac
     6361 ggagtttttc aatttaatga aaataaataa attatgaatg taaagttatt taaaatctta
     6421 ttggtaagtt tttttgtttt ctttttttta gattcgacaa tttatggatc aaatttaaat
     6481 gataaagcaa tcgaaaaaaa agtcgaaaag ttgctttcga aaatgacgct ggatgaaaaa
     6541 atcggccaga tgaatcaaat aagtatgttt gaggattcgg atcaactaaa tgtattgata
     6601 aaagaaggca gattaggctc tattattaat gtagcaaatc cagttgaaat aaattcattg
     6661 caacgagttg caattgaaga atctcgtctt ggtattccct tgctgatagg tcgggatgta
     6721 attcatgggt ttaaaactat ctttccaatt cctcttggtc aggctgcttc ttttgaccca
     6781 aatcttgtaa aaaatgccgc tcgagtagct gccatagaag cttcgtcagt tggtataaaa
     6841 tggacttttg ctccgatgat tgatatttct cgcgatccgc gttgggggcg aatagctgag
     6901 ggatttggtg aagatcctta tttgacatca gtaatgggaa ctgctatgat aaaaggcttt
     6961 caaggagata tattaaataa ttttaattct ataatggctt gtccaaaaca ttttgtcgct
     7021 tatggagcgg cagaaggagg acgtgactat aattctactt ttataccaga gcgccttttg
     7081 cggaatgtat atttacctcc ttttgaagct gccgttaaag ctggagcatc cagtattatg
     7141 tgttctttta atgataatga tggtgtgcct tcatctggca acgaatttat tttgaaacaa
     7201 gttttgcgaa aagaatgggg atttaatggt tttgtcgtct ctgattgggc ttctataaaa
     7261 gaaatgatta atcatggatt tgctgctgat gaaaaagaag ctgcaatgaa agcagtaaat
     7321 gcaggagtag atatggaaat ggtaagctta acgtatataa cttatttgaa agaattgatt
     7381 aaagaaggta aggtaaaaga aaaaactatt aatgatgctg tacgcaatat tttacgaatt
     7441 aaatttcggt tggggctttt tgatcatcct tacgttgacg aaaatcaatc atcagtaata
     7501 tatgctccat ctcatttagc tactgcaaag caattagctg ttgaatcgac tattctttta
     7561 aaaaatgctc acaatattct tcctcttact gatgaggtta aaacaattgc agtaattggt
     7621 ccaatggctg atgcacctta cgaacaaatg gggacatggg tatttgatgg agataaggca
     7681 tacactaaaa ctccgttaaa tgctttaaaa gaagaatatg gaaataaaat taatatcatt
     7741 tatgagtcag cgttggcata tactcgcgat aaaaatacag agggtattgc gaaagctgta
     7801 aaagctgctg aacaggccga cgtagtatta gcctttgtgg gcgaagaatc tattctttca
     7861 ggtgaagctc attgtttagc tgatttaaat ttacagggtg cccaatcgca attaatagaa
     7921 gcattatcga gtacaggcaa gcctattata acaattgtca tggcaggaag acctttaact
     7981 attggaaagg aagcagctct ttcatcagca gttttatatt ctttccatcc tggaacaatg
     8041 ggaggtccag ctattgtaga tttattattt gggaaagaag ttcctagtgg aaaaactcct
     8101 atcactttcc ctaaaatggt tggacaaatt ccgatttatt atgctcatac caacactgga
     8161 cgtcctccga caggaactga gaccatgttg gatcaaattc cagttgaagc cggacaaact
     8221 tcacttggaa acacttcttt ttatttagat gctggatttg aacctctgta tccctttggt
     8281 tatggcttgt cttatacaac ttttgaatat agtaatataa aactttcttc gtatgaatta
     8341 aaaaagaatg atatattaaa aataagtttt gatttgaaaa atacaggaaa atatgccgca
     8401 actgaagttg ctcagcttta tattcaagat aaagtgggct cggtaaccag accagtcaaa
     8461 gaattaaaac gttttgctcg tgtaacattg caaccgggag aaaaacggac tgtaactttt
     8521 gaacttccga ttagtgaact tgctttctgg aatattgata tgaaaaaagt agttgaagct
     8581 ggtgaattta atttatgggt tgccggagat agtcaaagtg gagaaaaaat ttcatttaaa
     8641 atagtagatt aattgttaat gttaagtagt ggttgttggt tgctaagtta attattaagt
     8701 ggttggtttt tttgtttttc ataggtggtg aagtgaaaag caggaaggtt aagaagactt
     8761 cctgcttttt gaatttttat accacgttac aatcaaatat aaggcgtata agtcaaaaag
     8821 tagactgaaa attttgctta agtagtattt cataaac
//
LOCUS       DAOSAS010000073         8783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_5230_length_8783_cov_13.049610, whole genome shotgun sequence.
ACCESSION   DAOSAS010000073 DAOSAS010000000
VERSION     DAOSAS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5230_length_8783_cov_13.049610"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /locus_tag="PLR72_04580"
     CDS             complement(<1..1664)
                     /locus_tag="PLR72_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOK36513.1"
                     /translation="MNFFNDNPALKFQLSHPLMQKIVTLKERNFVDKDKYDYASRDFE
                     DAIDNYEKILEIIGEIAGEVIASNAEEIDQTGPTVVNNRVIYAPGTQQNHETLIQAGV
                     YGMALPREYGGLNFAVIPYVMAAEIVSRADAGFANIWGLQDCAETINEFADEDIKREF
                     LPRVCAGQTCSMDLTEPDAGSDLQAVQLKATYNEKDGMWYLNGVKRFITNGDAHIKLV
                     LARSEEGTTDGRGLSLFVVDNKHDNTVKVRRIENKLGIKGSPTCELVFSNTPAKLIGS
                     RRLGLIKYVMSLMNEARLGIGAQSVGLSTAAYDEALSYAKERRQFGKAIIEFPAVFEM
                     ISLMKAKLDASRALLYETTRFVDIYKAYEAIEKERKLEPEEKNEYKEYQKLADAFTPV
                     IKLFASEYANQNAYDCVQIHGGSGFMKDYPCERLYRDARILTIYEGTSQLQVVAAIRH
                     VTTGTYLNQIRFYEQQPINPEFHSFRKRLITMTNLLENSINRVTETKNQEYIDFHARR
                     LVEMAGHIIMGYLLIIDASRNDMFRKSAEVYINYAEVETRKHATFI"
     gene            complement(1825..2844)
                     /locus_tag="PLR72_04585"
     CDS             complement(1825..2844)
                     /locus_tag="PLR72_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOK36514.1"
                     /translation="MNNIIVYLEIEDGVVRDISLELLTKGRTLAQQLDCKLEAVAIGY
                     QLDNIAKQVFPYGVDKLYVCDDKRFIPYTTLPHASVLINLFKQEKPQIALLGATTIGR
                     DLAPRVSSALFSGLTADCTALEIGDYEDKKAGKVYKNLLYQIRPAFGGNIVATIVNPE
                     CRPQMATVREGVMKKEIYDSKHTGEIHKLQVAKYVSDKDFVIEVLERNIEKSKINLKS
                     APVIVSGGYGVGSAENFKLLYELAEVLGGEVGASRAAVDAGFAEHERQIGQTGTTVRP
                     KLYIACGISGQIQHIAGMQESAMVISINSDPNAPINKIADYVITGTIEEVVPKMIKYY
                     KKNTK"
     gene            complement(2916..3788)
                     /locus_tag="PLR72_04590"
     CDS             complement(2916..3788)
                     /locus_tag="PLR72_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOK36515.1"
                     /translation="MSFNIVVLAKQVPDTRNVGKDAMKADGTVNRAALPAIFNPEDLN
                     ALEQALRLKSKFEGSTVKVLTMGPVRAAEILRESLYRGADEGYLLSGREFAGSDTLAT
                     SYALTCAIRKFDKVDIILCGRQAIDGDTAQVGPQVAEKMGFPQITYAEDILEVTENSI
                     TVKRRLERGVEIVRGPLPILITVNGTAAPCRPRNAKLTMKYKHAATPSEKQEMGADYT
                     DLYDTHPYLNLTEWGANDIEHDPRWLGLNGSPTKVKKIENIVFTVKESKRLTANDADI
                     DSLMKELINNHTLG"
     gene            complement(4282..5046)
                     /locus_tag="PLR72_04595"
     CDS             complement(4282..5046)
                     /locus_tag="PLR72_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOK36516.1"
                     /translation="MKTLISNSFVNMNGIKNFLFFLYQWLIAFPVIIVISIITALITI
                     LLSPIFPNKKISYFPARWWSRICCALCFVKVKILGTENIDPKQSYVFISNHQSLFDIF
                     AIYGWLPSIFKWLMKIELRKIPLVGTACELAGHIFIDRSNPIAAQKSIKKAEKKLKNG
                     VSVVIFPEGTRTRDGKLGKFKLGAFRIATDLSLPIVPITIRGSFERISRSTLKITPGT
                     IEMIIHQPIDVNAYLPNKVSELVKLSWEIIHADLEK"
     gene            complement(5027..5947)
                     /locus_tag="PLR72_04600"
     CDS             complement(5027..5947)
                     /locus_tag="PLR72_04600"
                     /inference="COORDINATES: protein motif:HMM:NF015132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOK36517.1"
                     /translation="MAKVKTFHLYIAITLAMIFWAMSFIWTKIALQSFRPITLITLRL
                     IIASILLLLIILLTGNTQRILKKDIKWFVLLAFFEPYLYYLGETFGLTKVSPTIASVI
                     ISTIPLFSPIFAFLFLKEKVGLWNILGIIVSLVGVCFVIYEPRTGFYSEPFGIILLFF
                     AVFSGVFYSIILKKISIRYSTLNIIFYQSVLGLLFFLPTFLIVDLPSIKTIQITHESI
                     LALILLAIFASVVAYVLFASVLRRIGVVRSNVFSNLIPAFTAIFTWLILREPLSILKL
                     LGIAIVISGLFVSQLNKLNLKKPKFENINF"
     gene            6107..6180
                     /locus_tag="PLR72_04605"
     tRNA            6107..6180
                     /locus_tag="PLR72_04605"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6141..6143,aa:Asn,seq:gtt)
     gene            complement(6221..7759)
                     /locus_tag="PLR72_04610"
     CDS             complement(6221..7759)
                     /locus_tag="PLR72_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOK36518.1"
                     /translation="MLVKTFGAAVQGINATIITIEVNVNQGIHFFLVGLPDNAVKESH
                     ERIASALDYIGYKMPRKQIVVNMAPADIRKEGSAYDLPLAIGMLAADEKIQANQLEQY
                     LIMGELSLDGSLNPIKGALSIAIKAREEKFKGFILPKQNAREAAVVNDLEVYGVENIK
                     QVVDFLNGNLDIEPTIVNTRQEFYENLNHFEIDFADVKGQENVKRALEVAAAGGHNII
                     MVGPPGSGKSMLAKRIPTILPPLTLHEALETTKIHSVAGKIGNNTSLMYERPFRSPHH
                     TISDVALVGGGTFPQPGEISLAHNGVLFLDELPEFKRSVLEVLRQPLEDRRITISRAK
                     FTIEYPASFMLIAAMNPCPCGYYNHPERECVCAPGVVQKYLNRISGPLLDRIDIHIEI
                     VPVPFEKLSDMKEGESSIKIRERVIKARQIQERRFKDIEGVYCNAQMTTKLQRKFATP
                     DKSGLELLKTAMQRLSLSARAYDRILKVARTIADLDDSEMILAHHIAEAINYRNLDRE
                     GWGS"
     gene            complement(7800..>8783)
                     /locus_tag="PLR72_04615"
     CDS             complement(7800..>8783)
                     /locus_tag="PLR72_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36519.1"
                     /translation="MGFNYFFDNTEFSGSSYVNDQTMKGLHVAPQLTFQWENIHSFFV
                     GTDLLNISGSKKAIEKVKLIAYYQLFTPKILLRVGSFPRSDILTNYSDFFFQDSVEYF
                     KPNIEGLFWQINHSSDFFNLWLDWTGHQTATQRESFFVGASGVKHFENFFADFQSYMF
                     HYANTAPRNTDFHVCDNVLMHLSLGINLLENRSKLDTLFFAVGLLQGFERERGLDNMT
                     FKPSGIVLRGNIQYNRFGSENSFYIGDKRMRLYNKHGNNLYWNNPFLRGNSYLESEWY
                     WKPIQTSTVEGKLALRLHLSEGKLFTEQLFTLKVLLDHSFKKNSKTIACQN"
BASE COUNT         2932 a         1622 c         1336 g         2893 t
ORIGIN      
        1 ttgataaagg ttgcatgttt gcgagtttcc acttcagcat aattaatata aacttcagcc
       61 gatttacgaa acatatcatt tcttgaagca tcaataatca ataaataccc cattataatg
      121 tgccctgcca tttcaaccaa tcggcgtgca tgaaaatcaa tatactcttg attctttgtt
      181 tcagttaccc tgttgatcga attttccaac aaattcgtca ttgttatcaa tcttttacga
      241 aacgaatgaa attctggatt aataggttgt tgctcgtaaa aacgaatttg atttaaatac
      301 gtacctgttg ttacatgacg aatagcagca acaacttgaa gctgagaagt tccttcataa
      361 atagtaagaa tacgtgcatc acgatatagt cgctcgcaag gataatcttt cataaatcca
      421 gaaccaccat gtatttgtac acaatcgtaa gcattctgat tggcatattc gctagcaaat
      481 aatttaatca ccggggtgaa ggcatctgcc aatttttgat actctttgta ttcatttttt
      541 tcttccggtt caagtttgcg ttctttttca atggcttcat aagctttata aatgtctaca
      601 aatcttgttg tttcataaag taaagctcgt gatgcatcca atttcgcttt catcaaagaa
      661 atcatttcaa atactgcagg aaattcgatg atagctttac caaattgtcg cctttctttt
      721 gcataagata aagcttcatc ataagctgct gttgatagtc caactgattg tgctccaata
      781 cccaaacgtg cttcattcat tagtgacatc acatatttaa ttaaccctaa acgacgagag
      841 ccaattaatt tagctggcgt gttgctgaaa accaattcac atgtcggtga gcctttgatt
      901 cccaatttat tttcaatacg tcgtactttt actgtgttat catgtttatt atcaacaaca
      961 aataaggata atccacgacc atcggttgta ccttcttcag agcgagccaa aaccagttta
     1021 atatgagcat caccattggt aataaaacgt tttacgccat tcaaatacca cattccatct
     1081 ttttcgttgt aagtagcttt cagttgaact gcttgtaggt cggaaccggc atcaggttca
     1141 gttaaatcca tggagcaagt ttgcccagca catacacgcg gcaaaaattc acgttttata
     1201 tcttcatcgg caaattcatt aatggtttca gcacaatctt gtaaacccca aatattagca
     1261 aaaccagcat cggcacgcga aacaatttca gcagccatta cataaggaat taccgcaaaa
     1321 tttaatcctc catattctcg tggtaaagcc attccatata ccccagcttg aataagagtt
     1381 tcgtgatttt gttgtgtacc tggtgcataa attacgcggt tattcactac agttggacct
     1441 gtttgatcta tttcttcggc gtttgaagcg ataacttcac cagcaatttc accaataatt
     1501 tccagaattt tctcataatt atcaatagca tcctcgaaat cgcgcgaggc ataatcatat
     1561 ttatctttat ctacaaaatt tcgttctttc aacgtgacaa ttttttgcat caacggatgc
     1621 gaaagttgaa attttaaagc tggattatca ttaaaaaaat tcatagcaat tgtttttctc
     1681 tttgattagt ttaaacgata aaaaaatcta aaataaattt tataaattta gttagtaatt
     1741 tattaattta atacttattt aatatgaatt ctgatttttt actaacggga gaaaatcctt
     1801 ttcagaaatt tttatcatac ttatttattt ggtatttttt ttataatatt taatcatctt
     1861 aggcactact tcttcgatgg ttccagtaat tacataatct gcaattttat taataggtgc
     1921 atttggatca ctgttaattg aaataaccat tgcactttcc tgcatccctg cgatatgttg
     1981 aatttgtcct gagattccac aagcgatata taattttggt ctcactgtgg tacctgtctg
     2041 accaatctga cgttcgtgtt ctgcaaaacc agcatcaact gctgctcgtg atgctcccac
     2101 ttcgcctccc aacacttcag ctaattcata taggagtttg aaattttctg cacttcctac
     2161 tccatagcct cctgatacaa taacgggggc agattttaaa tttatttttg atttttcaat
     2221 attacgttcc aatacttcta ttacaaaatc tttatcagaa acatattttg caacttgaag
     2281 cttatgaatt tcgcctgtat gttttgaatc atatatttct tttttcataa caccttcacg
     2341 cacagtagcc atttgtgggc gacattcagg attaacaata gtagcaacta tgtttccacc
     2401 aaaagcagga cgaatctgat aaagtaaatt tttatatact ttgcctgctt ttttatcttc
     2461 ataatctcca atttcgagag ctgtgcaatc ggctgttaag ccactgaata aagcagaaga
     2521 aacacgtggc gctaaatcgc ggcctattgt cgtggctccc aataatgcaa tttgtggttt
     2581 ttcttgcttg aaaagattaa ttaaaactga agcatgaggt aaagtagtat aaggtataaa
     2641 tcttttgtca tcacatacat ataacttgtc tacaccataa ggaaaaactt gcttagcaat
     2701 attatctaat tgatatccta ttgctacggc ttccagtttg caatctaatt gttgggcgag
     2761 agtacgtcct ttggtaagta attctaaact aatatctctc accaccccat cttcaatttc
     2821 taaatacaca attatattgt tcatgttcaa aatttttttc tattaaaaaa tttataagtt
     2881 tattgttttc atttgatagc ttaatcaata aaagattatc ccaatgtatg attgttaatc
     2941 aattccttca ttaagctgtc aatatcagca tcattagctg ttaaacgttt actttctttt
     3001 acagtaaaaa caatattctc aatttttttt actttagttg gtgagccgtt caaacctaac
     3061 cagcgaggat catgttcaat atcatttgct ccccattcag taagattcag ataaggatgg
     3121 gtatcatata aatcggtata atcggcaccc atttcttgtt tttcagacgg tgtagcagca
     3181 tgtttatatt tcatggtaag ttttgcattg cgtggacgac acggagcagc agtaccatta
     3241 acagtgatta atataggaag tggacctctt actatttcta ctcctctttc taaccgacgt
     3301 tttactgtaa tactattttc tgttacttct aaaatatctt ctgcataagt tatttgggga
     3361 aaacccattt tttcagctac ttgcggaccc acctgcgctg tgtcaccatc aattgcttgg
     3421 cgaccgcata aaataatgtc aactttatca aatttgcgaa tggcacaagt taaagcatac
     3481 gaagtagcca gggtatcaga accagcaaat tcacgcccac ttaataaata tccttcatct
     3541 gcaccacgat aaagactttc ccgaagaatt tctgctgcac gaacaggacc catagttaaa
     3601 actttcacag ttgatccttc gaatttactt tttaagcgta atgcttgttc caatgcattc
     3661 aaatcttctg gattaaaaat agcaggtaaa gctgctcgat tcacagtccc atctgctttc
     3721 atggcgtctt tccctacatt ccttgtatca ggtacttgtt ttgccaaaac tacaatgtta
     3781 aaactcatat cgataatatt atttcattaa attaattcct gtcacttctc cttactcctt
     3841 ataaatcatt ttgttttgat aaaaagtcag ttagcaaaga gttgtttaaa ttaagtttgc
     3901 aaaattagca gtttttattg aagaaacaag caattttatt ttattttcaa caataaagat
     3961 gacaaaaaac gcgaggcttg ggtaagtctc gcgcatacaa ttaggtatta gtttttttaa
     4021 ggtattaaaa agattgtgtt gtttagttaa cagttgcaaa atacgtatta aaaattgaaa
     4081 tttacaagtc aaaaaatatg ttttacaacc tattttatat aattttttaa aggaaaagaa
     4141 aaaagaaata accaaatatc taacataacc tatttaaaat cattagatta agttgtttat
     4201 cagttggttt ttaactgtta cctgttctta ttataaatac attagtttcc caaatgaaat
     4261 tgaacataaa tcattttttc ttcatttttc taagtcggca tgaatgattt cccagctaag
     4321 ttttaccagc tctgacactt tattgggtaa ataagcattt acatcaatag gttgatgaat
     4381 aatcatttca atggtgcctg gagtaatttt caacgtcgaa cgtgaaatgc gttcaaagct
     4441 ccctcgaatc gtaataggca caataggcaa cgacaagtca gtagcaatac gaaaagctcc
     4501 aagtttaaat tttcccaatt taccatcacg tgtacgggtt ccttctggaa aaatcaccac
     4561 ggaaacacca tttttcagtt ttttttcagc tttttttatg cttttctgtg ccgctatcgg
     4621 atttgaacga tcaataaaaa tatgacctgc caattcacag gcggtaccta ctaagggtat
     4681 ttttctcaat tctattttca tcaaccattt aaaaatggaa ggtaaccaac cataaatggc
     4741 aaaaatgtcg aacaagcttt gatgattgga aataaaaacg taagattgtt ttggatcaat
     4801 attttccgta ccaagtattt tcactttcac aaagcaaaga gcacaacaaa ttcgtgacca
     4861 ccaacgagcc ggaaaataag aaattttttt attaggaaaa atgggagata gcagtatagt
     4921 tatcaaagct gtaatgatag aaataacaat aattacaggg aaagctatta accattgata
     4981 aagaaaaaat agaaaatttt ttataccatt catgtttaca aaagaattag aaattaatgt
     5041 tttcaaattt aggtttcttt aagtttaact tattcaattg acttacaaat aaacctgaaa
     5101 tcactatggc gatacctaaa agtttcaata tagaaagagg ctctcttaaa atcaaccatg
     5161 tgaaaatagc ggtaaaagcc ggaattaaat tggaaaaaac attgcttcga acgacgccta
     5221 tacgtcttaa tacagaagca aacaatacat aagcaactac agaagcaaaa atagctaata
     5281 atattaatgc cagtatagat tcatgagtaa tttgaatagt ttttatgctc ggcaaatcaa
     5341 cgatcagaaa agtaggaaga aaaaataata accccaatac actttgataa aaaattatat
     5401 tcaaagtaga atagcgaatg gaaatttttt tcaaaataat cgaataaaat actccagaaa
     5461 aaacagcaaa aaacaggagt ataattccaa atggttcgct gtaaaaacca gttcgaggtt
     5521 cataaattac aaaacaaact cctaccagcg aaacaataat tcccagaata ttccatagcc
     5581 ctactttttc ttttaaaaag agaaaagcaa aaatgggaga aaataatgga atagtagaaa
     5641 tgataacaga agctatggta ggtgaaactt ttgtcaagcc aaaggtttca ccaagatagt
     5701 aaagataagg ttcaaaaaaa gcaagtaaaa caaaccactt aatatctttt tttaaaattc
     5761 gttgagtatt tccagtcagt aaaattatca aaagcaacaa aatagaggca ataattaatc
     5821 gtaaagttat caaagtaata gggcgaaaag actgtaaagc aatttttgtc cagataaaag
     5881 acattgccca aaatatcatc gctaaagtaa ttgcaatata taaatgaaaa gttttaactt
     5941 ttgccatatt ttataactgt ttcctctatt ttattgtttt caaaataaaa gtagataaaa
     6001 acctttaaaa attggtttaa ttttttactt caaaaaatat tctttttcac aaaaacattt
     6061 tgaaatattg gaaaaattag ttatttttgt ccgcactatt aaaaattctt ccttagctca
     6121 gtcggttaga gcatctgact gttaatcaga gggtccttgg ttcaagtcca agaggaagag
     6181 caaagttgct ttcattggca acttttttat tttgccaatt ttaacttccc caaccttctc
     6241 gatccagatt tcgataatta attgcttcgg caatatgatg agcaagaatc atttccgaat
     6301 catccaaatc tgcaatggtg cgagccactt tcaaaatccg atcataggct ctcgctgata
     6361 aactcaaacg ttgcatagct gtttttaata attcaagacc tgatttatca ggtgtggcaa
     6421 attttcgctg caacttggta gtcatctgag cattacaata aaccccctca atatctttaa
     6481 atcttctttc ctgaatttga cgagctttaa taaccctttc acggattttg atgctacttt
     6541 cgccttcttt catatctgaa agtttttcaa aaggaacagg tacaatttca atatgaatat
     6601 caatcctgtc caataaagga ccagaaatac gattcagata tttttggacg acacccggcg
     6661 cacaaacaca ttctcgttct ggatgattat aataaccgca agggcaggga ttcatagctg
     6721 ctatcagcat aaaactggca ggatattcaa tggtaaattt tgcacgtgaa atcgtaattc
     6781 ggcggtcttc aagaggctgt ctcaacactt caagaacaga acgcttaaat tcaggaagtt
     6841 catcaagaaa aagtacccca ttatgagcta gagaaatttc acctggctgc ggaaaagttc
     6901 ctcctcccac taaggcgaca tctgaaatag tatgatgtgg acttctgaaa ggacgttcat
     6961 acatcaaaga agtattattg ccaatttttc cagctacaga atgaattttt gttgtttcaa
     7021 gggcttcgtg taatgttaat ggtggcaaaa tggttggaat tcgctttgcc agcatagatt
     7081 taccagatcc aggaggacca accataataa tgttatgtcc acctgctgct gcaacttcta
     7141 aggctctttt tacattttcc tgacctttta catcagcaaa atcaatttca aaatgattca
     7201 aattttcata aaattcttgc cgcgtattga caatagtagg ttcaatatcc aaattaccat
     7261 ttagaaagtc aacaacctgc ttgatatttt ctacaccata aacttccagg tcgtttacta
     7321 cggcagcttc acgggcattt tgctttggca gaataaatcc tttgaatttt tcttctcgtg
     7381 ctttgatggc aatagacaaa gctcctttaa taggattcaa acttccatcc agcgacaatt
     7441 cacccataat aaggtattgt tctaattgat tggcttgaat tttttcatca gcagccagca
     7501 taccaatagc caatggaaga tcgtaagctg aaccttcttt gcgaatgtct gctggtgcca
     7561 tattcaccac tatctgcttt ctgggcattt tatagccaat ataatccaaa gcagaagcta
     7621 tgcgttcatg actttctttc actgcattat caggtaaacc taccaaaaag aaatggatac
     7681 cttgatttac atttacttca atagtgataa ttgtagcatt aatcccttga actgcagctc
     7741 caaacgtttt aactaacatt gataacgatt taaaattaaa attaaattta agccattttt
     7801 taattctgac aggcaattgt tttgctattt tttttaaaag aatggtcaag taatactttt
     7861 aatgtaaaca attgttcagt aaataatttc ccttcggata aatgaagtcg caaagccaat
     7921 ttcccttcca ctgttgaagt ttgaataggc ttccagtacc attcactttc cagataagaa
     7981 ttgccacgta aaaaaggatt attccaatat aaattatttc catgtttgtt atacaacctc
     8041 attcgtttat ctcctatata aaaagaattt tctgagccaa acctgttata ttgtatattt
     8101 cctctcaaaa caataccgct tggtttaaaa gtcatattat ccaaacctcg ttcacgttca
     8161 aatccttgta aaagacccac agcaaaaaac aaagtatcca attttgaacg attttccagc
     8221 aaatttattc ctaaagaaag atgcattaaa acattgtcac atacgtgaaa atcagtattt
     8281 cgaggagcag tattggcata atgaaacata tatgattgaa aatctgcgaa aaagttttca
     8341 aagtgtttta ctcctgaagc accaacaaag aatgattcac gctgggttgc agtttgatgt
     8401 cctgtccaat cgagccacaa attaaaaaaa tcggatgaat ggtttatttg ccaaaacaat
     8461 ccttctatat tcggtttaaa gtattctaca gaatcttgaa aaaagaaatc ggaataattt
     8521 gtaaggatat cagaacgggg aaaagaacct acacgcaata aaatttttgg tgtaaataat
     8581 tgataataag ctattaattt aactttttct attgcttttt ttgaacccga tatatttagc
     8641 aaatctgtcc ccacaaaaaa agaatgaata ttttcccatt gaaaggttaa ctgaggagcg
     8701 acgtgcaatc ctttcatagt ctgatcattc acataagaag atcctgaaaa ttcggtattg
     8761 tcaaaaaaat aattaaaacc cat
//
LOCUS       DAOSAS010000074         8743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_5264_length_8743_cov_11.641575, whole genome shotgun sequence.
ACCESSION   DAOSAS010000074 DAOSAS010000000
VERSION     DAOSAS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5264_length_8743_cov_11.641575"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(688..1644)
                     /locus_tag="PLR72_04620"
     CDS             complement(688..1644)
                     /locus_tag="PLR72_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36520.1"
                     /translation="MKKIKLIIGFFFLNFVLFAQNNQGKSDDAGRIVLTSVVSDQIEG
                     LNPSAQSNLHNKLNQIATKNGMGGSSLNNRFIITANVVVLTKDVTATAPPMHAYTLEI
                     TLYIGDGIEGTLFSSTSVTLKGVGETETKAYMAALKNLKVDDPKYQSFIEQGKNKIIE
                     YYNSKCDFILKEAEMLAGQNEFDAAISKLVDIPEVCKECYDKALTAIQPIYQKKIDRD
                     CKIKLQEAQGIWNAVQDRSSAEKAGAILSTIEPDASCFAEVQALNKKIADKVKQIDDR
                     EWNYILKEQAQESERIEAYRAIGVAYGNNQPQNVTYNYKGWW"
     gene            complement(1663..2607)
                     /locus_tag="PLR72_04625"
     CDS             complement(1663..2607)
                     /locus_tag="PLR72_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HOK36521.1"
                     /translation="MKHLIFAFLLVLCTTVAFSQAKKPTIMVVPSDVWCNINGYMMEF
                     NNQGKVVKVPDYKKALQENADLLLVISKINELMAERGFPLKNLESSLKSLESEAAEDA
                     MLTSKGGSEVNESPIDKLKKVAKADIWIQMTWTVNTTGPKKSITFNLQGLDAYTDKQI
                     AGASGTGEPSFTSELPVLLEEAVLAHLDNFNVQLQSHFDDMLTNGREIILRIKTWNAW
                     EYDLESEDFGNDELSSLIENWVTDNTVQGRFNTTDATENMMLFEQVRIPLYDEAGKAL
                     DARKWANGLRKYLKDTYQIESKLMMKGLGEAQLVLGEK"
     gene            complement(2619..3098)
                     /locus_tag="PLR72_04630"
     CDS             complement(2619..3098)
                     /locus_tag="PLR72_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36522.1"
                     /translation="MILFAISIVLISCNAQNKITWNYSYKTECLGIEMDGSQTVKAWG
                     NGRNRLDAVEQAKKNAVNDVLFNGIYEGKQDCEKRPVVAEVNARQKYETYFNKFFADD
                     GEYKKYVSLKDERIGQKVSRDRKGARHSVTHGVVVRVLRAELKQKMIEDGIIKNQQF"
     gene            complement(3136..3651)
                     /locus_tag="PLR72_04635"
     CDS             complement(3136..3651)
                     /locus_tag="PLR72_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36523.1"
                     /translation="MKKINILMMFVAFLTLPYFSMAQSKKKADNETVEWRYEIEAVGI
                     GTQGTYQIKVWSYSKNPETAIEQAKKNAVHGIIFKGFPSKDRIPGQKALAQSPNLEQE
                     KEDFFKDFFQDGGKYQKYVTLTNNGAIAPGDRIKIGKEYKIGVVVSVNVAALRKDLED
                     AGIIKGLGSGF"
     gene            complement(3680..5032)
                     /locus_tag="PLR72_04640"
     CDS             complement(3680..5032)
                     /locus_tag="PLR72_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36524.1"
                     /translation="MKKLIYTVSTVICLVVTIQTFAQESSKDFKYRRSSLAMILIESD
                     NFPNKDAVMSSWNNYPFPDKYNKHSIDLKSININSIELSEQDLLNAGFLKDTLKDPLQ
                     IAKATGSLKPVKYLNAEQTIAVVMPTEKQEYQLKIDKFINDNKLANMLVATWFNRSAD
                     GKFDMSLIQERGFYNASELEASIAKGQTKGLASLGDAGEELIRNTFVTFTKLEFIENE
                     PIAALVRDVTKAEIAESMAGKPQILIDKALQAADVTYEKTKEGYSLWSKTWLYRLNWN
                     DSIASIFYNDLWSNPSAFDKSDLFSLEFVGVQYNQSLVTFKIGESRTQEQLIDLALVR
                     NVDNAFAELQKDNDVFKPKVPVLTVNPITAQIGMKEGLKGGESFDVLEMTLNPKTGLT
                     EYKKVGSATADKKIIWDNRYNAGEKVEQVLDKDGNPITATHFKGGKSIQPGMLLKQVK
                     "
     gene            complement(5192..5569)
                     /locus_tag="PLR72_04645"
     CDS             complement(5192..5569)
                     /locus_tag="PLR72_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOK36525.1"
                     /translation="MENLEHTESIEQEDKIYISNSIFKNLPLMVQKELSKMTLEKQEE
                     FVEEFKRKSKSVGMAYLFLFIIFSAHYAYLKKWGIQILYWVTVGGAGIWFIICIFIIP
                     NLVRNYNKEIAIDILKDLKIISN"
     gene            complement(5589..6413)
                     /locus_tag="PLR72_04650"
     CDS             complement(5589..6413)
                     /locus_tag="PLR72_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36526.1"
                     /translation="MKKLMKILGVILFASVILTSCGGSKSKNAKSAKEFSIKPITTYV
                     KGDLSDFFEVVDGTYKLEKAEGSFSDFKLKVQLKRKEPAFDFDAKDLESRGYFNLYCN
                     FLDEGGTPIIQGVRAYEGMKEITTLKPGETGWVEFSFSPRYQNYDIEKVTTFEINSTV
                     NKGMSQSNTSNYSEDTETGDEVSNTSNDKKNKTTSVDYNKFLNDYENLVNKYINLVKK
                     VNAGDISAMDDAYDILEEAESLAYDLEDAKDEMTTAQMQRFVKIQAKLAKAINELE"
     gene            complement(6528..7382)
                     /locus_tag="PLR72_04655"
     CDS             complement(6528..7382)
                     /locus_tag="PLR72_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36527.1"
                     /translation="MKNINLFLTIILGFVMIFLNSCSSPESEGKKLGDKFCNCLGDFK
                     TFTEPKQFNQVMDSCKNLIKDDWTKYENDYKKNESKWNLFNTSYEKACESKLKEFNDS
                     LKLIYAEIEKKIKENLNDKLWLKKDENKGYYLYSFNDNALSIINCKGESKFKLSVDTI
                     KFEDTESTMAIVSFTEDGNLILTDCKSGKKGVYQPASEKDKLLGNWKGFSEFISFYPG
                     GNCYVSTDWGAYKKHGKYSFIDNNLEIDGPPSYRVNFSNIDYFSWGQIQFYRNKYAHP
                     KNLSDVLF"
     gene            complement(8325..>8743)
                     /locus_tag="PLR72_04660"
     CDS             complement(8325..>8743)
                     /locus_tag="PLR72_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36528.1"
                     /translation="IQQSSDITYRIYDWDRPQDSDNPRPLHIDKALQVIDYDAQLEDL
                     LYQPFLADKTVNLFNGKDFVTNRVVLSAGREIIKNVELFDSFFVYLFLNGNGEIFVNE
                     HQIDFLAGDCCMIPASFTEIHLRAYSDIQALEIYIP"
BASE COUNT         2730 a         1518 c         1116 g         3379 t
ORIGIN      
        1 tcctgtagct cctgttaatt catatcaagc tgtttcgcct tctttttatt ttctgccaga
       61 tgcttcagca ctagaattaa caaacaatct tttacatgaa atagttggat tattagcata
      121 taaaataatg gggtatgctt gataaaatta aattaatatt ttgaattacg cgtttagata
      181 ataaagtttt ctaaatattt ctgtatttaa agaataactt taaaatttat tttttgctcg
      241 aattaatacc gcattgggaa atgatttaca ataaaacaag caagcagcgt aaggtaaaag
      301 ggtttacact gtttgagcaa gctatagctt gtgagtttgt aaacccaaat gggtgattgc
      361 gtagttttat caaatcattt ccccagcgga gatttttatt tttgtggctt cgatactagc
      421 ttcgctctac tcagccacca ataaagaaag aatttaacaa gaaatatttt aaataaatca
      481 aattaaactc atagaaaatc tagtcgatgc tgatacattc acaataataa attagttttt
      541 atttaaacgt aaaatttgaa aaattaaaat aaataaaact aatttttact gtgtgttagc
      601 tttgcaagct ctttgtcaaa gttttgattg tagcgatgac ttatgggact ttggcaatgt
      661 gcttgaaaag ttttctgcac attcatgcta ccaccaacct ttataattat aagtcacatt
      721 ttggggctga ttattaccat atgcaacacc aattgctcta tatgcttcaa tacgttcact
      781 ttcttgagct tgttctttta gaatatagtt ccattcccta tcatctattt gcttcacttt
      841 atcagcgatc tttttattta atgcttggac ttcagcaaaa catgaggcat ctggttcaat
      901 agttgataaa atagctcctg ctttttctgc cgaacttctg tcttgcactg cattccaaat
      961 accctgtgct tcctgtaatt tgattttgca atctctatca atcttttttt gataaattgg
     1021 ttgtatagca gttaatgctt tatcgtaaca ttctttacaa acctctggta tatctaccaa
     1081 ttttgatatc gcagcatcaa attcgttttg ccctgctagc atttcagcct cttttagtat
     1141 aaaatcacat ttactattat aatactctat gattttattt ttcccttgtt caataaagct
     1201 ttggtatttt gggtcatcaa cctttaggtt ttttagtgca gccatatatg ctttggtttc
     1261 tgtttcacca acacccttta gtgtaacaga agtacttgag aataaagtac cttcaatacc
     1321 atctccaatg taaagagtta tttctaaggt atatgcgtgc atcggaggtg ctgtagctgt
     1381 aacatctttc gttaatacaa ctacattggc tgtaataata aaacgattat ttaaagaact
     1441 accacccata ccattttttg tagcaatttg atttaatttg ttatgcaaat ttgattgagc
     1501 agaagggttt aacccttcaa tttgatctga aacaaccgat gttagtacaa tacgtcctgc
     1561 atcatctgat tttccttgat tattttgtgc aaacaaaaca aaattcaaga aaaaaaatcc
     1621 tattattaat tttattttct tcatttctgt aattttttag atttattttt cccctaaaac
     1681 caattgagcc tctccaagtc ctttcatcat taattttgat tcaatttggt aggtgtcttt
     1741 taaatatttt cttagaccat tagcccattt tcttgcatct aatgctttac ctgcttcatc
     1801 gtaaagagga atacgaactt gttcaaataa catcatattt tcagttgcat cagttgtatt
     1861 aaatctacct tgtacagtat tatctgtaac ccaattttca ataaggctgc taagttcatc
     1921 atttccaaaa tcttcacttt ctaagtcata ctcccatgca ttccatgttt taatacgtag
     1981 aataatctct ctaccattag taagcatatc atcaaaatgg ctttgaagct gtacattaaa
     2041 attgtcaagg tgggcaagaa ctgcttcctc taataatact ggcaattcag atgtaaatga
     2101 tggctcacca gtgccagaag ccccagcaat ctgtttatcc gtataagcat ctaatccttg
     2161 taaattaaaa gtaattgatt ttttgggtcc tgttgtgtta actgtccatg tcatttgtat
     2221 ccatatatct gcttttgcaa ctttctttaa tttatcaatc ggactttcat taacctcact
     2281 gccaccttta cttgttaaca ttgcgtcttc tgcggcttca ctttctaatg acttaagaga
     2341 cgattccaaa tttttaagtg gaaatcctct ctctgccatt aattcattta ttttactaat
     2401 aactaatagc aaatccgcat tttcttgtag ggctttttta taatcaggga ctttaacaac
     2461 tttcccttga ttattaaact ccatcatata gccatttata ttgcaccaaa catcacttgg
     2521 aacaaccatg attgttggtt ttttagcttg tgaaaaagct actgttgtgc ataaaaccaa
     2581 taaaaatgca aagattaaat gtttcatttt tctataattt aaaattgttg atttttgatt
     2641 attccatcct caatcatttt ttgtttgagt tctgcacgga ggactcgtac aacaacacca
     2701 tgagtaacac tatgacgtgc acccttacgg tctcttgata ctttttgacc aatacgttcg
     2761 tcttttaatg atacatattt tttatattca ccatcatcag cgaaaaattt attaaaatat
     2821 gtttcatatt tttgccgagc attaacttct gcaacaacag gtcttttttc acaatcttgt
     2881 tttccttcat aaataccatt gaaaaggaca tcgtttacag catttttctt ggcttgctct
     2941 acagcatcca atctgtttcg tccattgccc catgccttaa cagtttgcga accatccatt
     3001 tcaatcccaa gacattctgt tttgtaggag tagttccaag taattttatt ttgagcatta
     3061 caggaaataa gaactattga tattgcaaaa agtatcattg cgtatattaa atatttcata
     3121 atttgtgtta tttagttaaa aaccactacc aaggcctttt ataattcctg catcttccaa
     3181 atcttttcga agtgcagcaa cattaacaga aaccacaact ccgattttat actcttttcc
     3241 aatttttatt ctatcacctg gtgcaatagc tccattattg gtaagagtaa catatttttg
     3301 gtattttcct ccatcttgga aaaaatcctt aaaaaaatct tctttttcct gttccaaatt
     3361 aggactttgt gccaaagctt tttgtccagg aatccgatct ttagagggaa aacctttaaa
     3421 aattattcca tgaacagcat ttttttttgc ttgttctatg gctgtttcag gatttttaga
     3481 atacgaccat acttttatct gataagttcc ctgagtacca attccaactg cttcaatttc
     3541 atatctccat tcaacagttt cgttgtcggc tttctttttt gactgagcca tactaaagta
     3601 tggcaaagtc aaaaatgcga caaacatcat tagtatgttt atctttttca tattgatttc
     3661 tttttttttt taacaatttt tattttactt gttttaataa cattccaggc tgtatagatt
     3721 taccaccttt aaaatgggta gcagtaatag gattaccatc tttatcaagc acttgctcta
     3781 ctttttcacc tgcattatat ctattatccc agattatttt cttgtctgct gtagctgaac
     3841 caactttttt atattctgtt aatccagtct taggatttaa tgtcatttca agtacatcaa
     3901 aactttcacc tccttttaaa ccctctttca ttccaatttg agccgtaatc ggatttactg
     3961 ttaaaacagg aactttaggt ttaaatacat cattatcttt ttgtaattca gcaaaagcat
     4021 tgtcaacatt tcttactaat gctaaatcga tcagttgctc ttgtgttcta ctttctccta
     4081 ttttaaaagt aaccaagctt tgattatact gtacaccaac aaattcaaga ctaaataaat
     4141 cagacttatc aaaagcagaa ggatttgacc aaaggtcatt atagaaaata cttgcaattg
     4201 aatcattcca gtttaatcta tacaaccatg ttttagacca taaagaatac ccttcttttg
     4261 ttttctcata tgtaacatca gcagcttgta atgccttatc tattaatatt tgtggtttac
     4321 ctgccataga ttcagctatt tctgcttttg ttacatcacg cacaagagct gctattggtt
     4381 cgttttcgat aaattctaat tttgtgaaag taacaaaagt atttctaatc aattcttcac
     4441 cagcatcacc aagggatgcc aatccttttg tttgaccttt tgcaatactg gcttctaatt
     4501 ctgatgcatt ataaaatcct cgctcttgga ttaaactcat atcaaattta ccgtcagcac
     4561 tcctattaaa ccatgttgct actaacatat ttgccaactt attgtcattt atgaatttat
     4621 caatttttaa ttgatattct tgtttctcag tcggcataac aactgcaata gtttgttcag
     4681 catttagata cttaacgggt tttaaactcc ctgttgcttt tgcaatttga agaggatcct
     4741 ttaaggtgtc tttcaaaaat cctgcattaa gcaaatcttg ttcggataat tcaatagagt
     4801 taatatttat tgattttaaa tcaatactat gcttattgta tttgtctgga aatgggtaat
     4861 tattccatga actcatcaca gcatctttgt ttgggaaatt atctgattca attagaatca
     4921 ttgctaatga acttctgcga tatttaaagt ctttgctgga ctcttgggca aatgtttgaa
     4981 tagttactac caaacatatt actgttgaaa ctgtgtaaat taattttttc atttttttaa
     5041 atttttacat ttcccctact cttttggctt ttcgggtttc gccccattat tttaagtagt
     5101 ttatggattc tacctgataa aaaatttttt ttcataatca taatatataa attaagtcca
     5161 tacttttagg tagttaacaa cactttgata tttaattcga aataattttt aaatctttta
     5221 aaatatcaat tgcaatttct ttattataat ttctgactag attaggaatg ataaatatgc
     5281 atattataaa ccatattcct gcaccaccta cagtaaccca atataatatt tgaattcccc
     5341 atttttttaa atatgcgtaa tgagctgaaa atattataaa taaaaataaa taagccatcc
     5401 ctacggattt agattttctt ttaaactctt caacaaattc ttcttgcttt tcaagtgtca
     5461 ttttggataa ttccttttgt accattaaag gtagattttt aaaaattgaa ttagaaatat
     5521 atattttgtc ttcttgttca attgattcag tgtgttctaa attttccata atattaattt
     5581 ttaattattt attctaattc atttatagct tttgctaatt ttgcttgaat ttttacaaat
     5641 ctttgcattt gtgctgttgt catttcatcc ttagcatctt ctaaatcata tgctaatgat
     5701 tcagcttcct ccaaaatatc ataagcatca tccatcgctg aaatatcgcc agcatttact
     5761 ttttttacta agtttatata tttattaact aaattttcat aatcatttaa aaatttatta
     5821 taatctacag aagtagtttt atttttttta tcgtttgatg tattgcttac ttcatcaccg
     5881 gtttcggtgt cttctgaata gtttgatgta ttgctttgac tcatcccttt attaactgta
     5941 gaatttatct caaaggttgt tactttttca atatcgtaat tctggtaacg aggtgaaaat
     6001 gaaaattcaa cccaacctgt ttcgccgggt tttagagttg ttatttcttt cattccctca
     6061 taagctctaa ccccctgaat tattggggtt cccccttcgt ctaaaaaatt acaatacaaa
     6121 ttaaaatacc ctcttgactc caaatctttt gcatcgaaat caaaagctgg ttcctttctt
     6181 tttaattgaa ctttaagttt gaaatcagaa aaagatcctt ctgctttttc aagtttataa
     6241 gttccgtcaa cgacttcaaa aaaatcggac aaatctcctt ttacataagt tgtaatgggt
     6301 ttaatgctaa attcttttgc acttttagca tttttacttt ttgaaccccc acaacttgta
     6361 aggattacgg aagcaaataa tattactccc aaaattttca ttaatttttt cattgttttt
     6421 aaatttttat attttcccta ctcttttggc ttttcgggtt tcgccccatt atttatttag
     6481 tggtttatgg ttaccaatga aatattatat atttattttt aattgtttta aaaaagtaca
     6541 tcacttaaat ttttagggtg agcatactta ttacgataaa attgaatttg accccatgag
     6601 aagtaatcta tattcgaaaa gttgactcta tatgaaggag gtccatctat ttcaagatta
     6661 ttatctatga aagaatactt tccatgtttt ttatatgctc cccaatctgt tgaaacatag
     6721 cagttacctc ctgggtaaaa agatataaac tcactaaatc ctttccaatt tccaagaagt
     6781 ttgtcttttt cagaagcagg ttgataaaca ccttttttgc cacttttaca atctgtcaaa
     6841 attaaatttc cgtcttctgt aaagctaaca atggccatcg ttgattcagt atcttcaaac
     6901 tttattgtat caacacttag tttgaatttt gattcacctt tacaattgat tattgacaaa
     6961 gcgttgtcat taaatgaata taaataatac cctttatttt catctttttt taaccataat
     7021 ttgtcattaa gattttcctt aatttttttt tcaatttcag cataaattag tttaagtgag
     7081 tcattgaatt cttttaactt gctttcacaa gccttttcat aagatgtatt aaataagttc
     7141 catttagatt catttttttt ataatcattt tcgtattttg tccagtcgtc ttttattaaa
     7201 tttttacaac tatccataac ttgattaaat tgtttgggtt ctgtaaaagt tttaaaatca
     7261 cctaaacaat tacagaattt atcacctaat tttttccctt cactttcggg agaagaacaa
     7321 gaattaagga atatcatcac gaatcccaaa ataattgtca aaaataaatt aatatttttc
     7381 attttttttc attttaaaat ttcctttaaa ataaaggttt tcagaatata gcccattttt
     7441 tataatgggt tctgatctcc actttttttg tttttttgcc ttggaggttc aaaaggcagg
     7501 gtaataattt ttattaaaaa cataattaaa tataataaaa aagggcgtga acctcacact
     7561 ttcattcaac ttgttttagg tatcgccaga cacccatacg tgattgaaat cagttagaaa
     7621 ttacacgccc ctgggggcgc attttccaac ttttttccac gtattatgaa aactggcgat
     7681 tttaaaacaa gaaaaacgtt agtaacgcaa ttttttatta tatttcaaag aacgattttt
     7741 ctattaaaaa aagcaaatat aatgcttttt taataaaact aaataaaaaa taacctcctt
     7801 tctcttttaa agtgggttct aaaaaattcc ttttattatt atgattttaa aaaatcgtag
     7861 aacccattaa taaattttct aaaatcgatg caaatatagt aagatttttt aaattgtata
     7921 aaaaattttt cttaattata aaaaaatatt gttataaaga atttttatta agttaaaaat
     7981 agatatataa gaaaatattg ataactatta caactcatat tattactata aatatttata
     8041 attgtaatat ttttattaat tgaaataggc gtttagataa taaagtcagt ggttcagaca
     8101 atctttgctg gcttcgatac gccctatcgg gctactctgc cagcaaaaaa cgtaatgatg
     8161 gtggctgagt agagcgaagc tcgtatcaaa gccaccatta aagaaaagtt ttaaaataaa
     8221 atttttttaa acaaatcaaa ttacactctt ataaaatata ccttcgctta tatatttaca
     8281 ataataattt tgttattact taaaccacta tttgaataaa tattttacgg aatataaatt
     8341 tctaaagctt gtatatcact ataagcacgc agatgaattt cagtgaacga agcaggtatc
     8401 atacagcaat ctccagctaa aaaatcaatt tgatgctcat taacaaatat ttctccattg
     8461 ccatttaaaa aaagataaac aaaaaaacta tcaaaaagtt ctacattttt tataatctct
     8521 ctaccagcag ataggactac tctattagtt acgaaatctt ttccattaaa taaattcaca
     8581 gttttgtctg ctaaaaaagg ctgatacaga aggtcctcga gttgagcatc gtagtctatc
     8641 acttgcagag ctttatctat atgcaatggt ctaggattgt ctgaatcttg tggtctatcc
     8701 caatcataaa tacgataagt aatatcacta ctttgctgta tct
//
LOCUS       DAOSAS010000075         8644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_5332_length_8644_cov_12.553848, whole genome shotgun sequence.
ACCESSION   DAOSAS010000075 DAOSAS010000000
VERSION     DAOSAS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8644
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5332_length_8644_cov_12.553848"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..697
                     /locus_tag="PLR72_04665"
     CDS             125..697
                     /locus_tag="PLR72_04665"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOK36529.1"
                     /translation="MKILVFDNYDSFTYNLVHAVKKLGYDDVEVHRNDRIQLVDIDRF
                     DKILLSPGPGLPAESGILLEVVKRYASSKSILGVCLGEQAIAEAFGGTLLNLPEVYHG
                     VCSTIEIMKKDYLFNGLPQKIEAGRYHSWIVEKETLPSCLEVTAVDNQGMIMAIKHKI
                     YDVRGLQFHPESVLTPLGEKILKNWLQNTH"
     gene            728..1726
                     /gene="trpD"
                     /locus_tag="PLR72_04670"
     CDS             728..1726
                     /gene="trpD"
                     /locus_tag="PLR72_04670"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOK36530.1"
                     /translation="MKEILNRLFDHQSLTREEAAEVMSNIGAGKYNESQMAAFLSVYL
                     MRSIELDELIGFRDALMTMAIPMDLSEFDPIDIVGTGGDGKNTFNISTCSCFTLAGAG
                     YKVAKHGNYGATSISGASNVIEYYGAKFTTDIDIAKRSLEESGFAYLHAPLCNPAMKN
                     VAPVRKNIGVRTFFNVLGPLISPVRPRYQCLGVYNLKMMRLYNYLYQKLGIQYSIVYT
                     FDGYDEISLTDKCKIINSEGEFIYTPEQLGFEKVNPAELWGGETVEHAAKIFMNILDN
                     QSTDGQRNVVIINSAFAIKTLCPEKAIEECKAEAIESLASGKAREAFMKFEEIYHL"
     gene            1736..>2136
                     /locus_tag="PLR72_04675"
     CDS             1736..>2136
                     /locus_tag="PLR72_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HOK36531.1"
                     /translation="MEEKNEQKNSTIQKTDFLENILRNKREEVVLKKQSFKGKNWENF
                     PFFSRKCFSLKQNLLSLPIGIIAEFKRKSPSKGWLNQQADLFKVTTDYQQAGVAGISI
                     LTDTEFFGGSVEDVITVRDKISCPILRKDFVV"
     assembly_gap    2137..2236
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2326..3009
                     /locus_tag="PLR72_04680"
     CDS             2326..3009
                     /locus_tag="PLR72_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOK36532.1"
                     /translation="MKDNEKGREENNEVSQIANFKLIKVCGMKFPENIREVAALQPDF
                     MGFVFYKKSPRYVRHLDISVLDSLPSSIKKMGVFVNEKLDNILSTVYKYHLDGVQLHG
                     SELEDKCEKLKEAGLLVIKAFPVESSYNFIVTKPYESVCDYFLFDTKVPIYGGAGIKF
                     DWSLLNDYKGNTPFFLSGGISLEDVEDLKKIEHPKFIGVDLNSKFEIAPGKKNVELLR
                     QFFQLYRYS"
     gene            3030..3800
                     /gene="trpA"
                     /locus_tag="PLR72_04685"
     CDS             3030..3800
                     /gene="trpA"
                     /locus_tag="PLR72_04685"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOK36533.1"
                     /translation="MNRIQQLFQQKKENILSVYFTAGFPNLNDTVPVLESLQRKGIDL
                     VEIGIPFSDPLADGPVIQNSSQIALRNGMSLRKLFEQLTDVRSKIDIPLIMMGYLNPI
                     MQFGFENFCQECHRVGVDGMIIPDLPMDDYLTEYKDIAQKYGLEFVFLITPETSEKRI
                     RQIDEHTNGFIYMVSSPGVTGTRNSFDDHIDYFNRINSMHLKNPRLIGFGISNKATRE
                     TACQYSSGVIIGSAFIKALQETNDIDQAVDLLLERLNK"
     assembly_gap    3811..3910
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4061..>5070
                     /locus_tag="PLR72_04690"
     CDS             4061..>5070
                     /locus_tag="PLR72_04690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOK36534.1"
                     /translation="MQLPTAFEERTRLLLGEEYPAFIKALNETPPTSIRVNDKMNYVP
                     SSQKVAWCESGYYLPERPSFTSDPLFHAGVYYVQEASSMFLQQAINQHFPEAKNILDV
                     CAAPGGKTTLLSQSFDNDTLIVSNEIIHSRVKILAENVIKWGNANVIVTNNEPADFSK
                     LSSFFDTVIVDAPCSGEGLFRKNPAAIQEWSVENVQKCAVRQKKIVQEVWDALKTDGI
                     LVYSTCTYNREENEENVFWICKELGAELLNLDVSRWKNITVSDGGYRFYPHKIRGEGF
                     FLSILRKTAKTPIRNYSFKKKETSKTQPISLPSISLKRPENWIFELNNQQLKAYPKNR
                     WK"
     assembly_gap    5071..5170
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5345..6097
                     /locus_tag="PLR72_04695"
     CDS             5345..6097
                     /locus_tag="PLR72_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK36535.1"
                     /translation="MKLLFLGTGTSTGIPQIGCQCKTCQSTDRKDKRLRASLLVNVQK
                     TNILIDAGPDLREQLLTYQISKLSGILLTHEHYDHIGGIDDLRPLGESHLYAEKRVLN
                     IIQKNMPYCFGDNLYPGVPMLQLHEITEKSKFYIDQVEIQAVRVMHAKLPILGFRIGN
                     IAYLTDVKTLDNKAIEQLQYLDLLVINALRQFPHISHLSLQEAIELSEKINAKETYFT
                     HISHDMGKHADVEAHLPSHIHLAYDGLEIEIN"
     gene            6114..7121
                     /locus_tag="PLR72_04700"
     CDS             6114..7121
                     /locus_tag="PLR72_04700"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOK36536.1"
                     /translation="MKKCSYLIVIFINILLSSCSSLLFTTLDVLRPAQVAFPPEVNNL
                     LIVNNTPQDSIQPSKKSFTKDSLSIYCLSALTEELINRGFFQNVLLELNSLNKTKNDT
                     SIYYLTPEVVTQLCSNYKSDAILALNKLKETDILTEYFVEDENSFLSTFEVFIETYWS
                     IHYPDKTDFTVLQFKDSMYWESENYSRQAALAQLPNKIDAQIDAALYAGRNCVKRFVP
                     YWEKVDRYFFNPRNKLMRQAMDSVYYKNWEAAIKLWDKAIQTTKNTKLQAQAANNIAI
                     AYEIFGDLDKAIEYATLSLKSFLNVTTVDKETIIRISNYRNELIKRKEEIELLEIQLG
                     E"
     gene            7182..>8644
                     /locus_tag="PLR72_04705"
     CDS             7182..>8644
                     /locus_tag="PLR72_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOK36537.1"
                     /translation="MIHLSPFDEITLREKGLTISQIEEQLRFFETGFPFLEIVRPATV
                     DDGILKIQENEKKKLIALWDKYLYQSKIEIMKFVPASGAASRMFKDLYEFLESKEEIP
                     SNGVIRQFFSEIDKFAFYDLLNETCVKNTGKNIQMLIQEKKYKIIIENLLNERGLNYG
                     SLPKGLLLFHTYPDEKRTPVQEHLVEGALYTVNAQKEVNIHFTVSPQHKALFEKHISE
                     VKEKYEKKFKVHYSVSFSEQKPSTDTIAVDMNNQPIRENGKLVFRPGGHGALIENLND
                     LNSDIIFIKNIDNVVPDALKGPTVTYKKILAGLLTNVQHQCFQYLNELENKNISERKL
                     NEILQFTEKTLNIYHPNISAFDICSLRNYLITKLNRPLRVCGMVKNVGEPGGGPFFIV
                     NADGTISLQILESAQINTQNPADKEKMERSTHFNPVDLVCAVKNYKGEKFNLLNYVDK
                     KAGFISIKTKNGKKLKALELPGLWNGAMSDWNTIFVEV"
BASE COUNT         2922 a         1236 c         1515 g         2671 t
ORIGIN      
        1 aacgaagaaa atgaattgca ggaagtaaac aataaattgg ctgcactcaa acaagctatt
       61 gaagtagctt cgagtgtctc gatttaaaaa aattgagtta ttattctgaa aaattgaaag
      121 ttagatgaaa atacttgtgt ttgataatta tgattctttt acctacaatc tggtgcatgc
      181 cgtaaaaaaa ttaggatacg acgatgtgga agtgcatcga aatgatcgca ttcagttggt
      241 cgatattgat cgttttgata aaattcttct ttcgcctggt ccaggtttac cagcagaatc
      301 aggaatttta ttggaagttg ttaaaagata tgcttccagc aaaagtattt taggtgtttg
      361 cctgggagaa caggcaattg cagaagcttt tggaggaacg ttgttaaatc ttcctgaagt
      421 atatcatggc gtttgttcta ctattgaaat tatgaaaaag gattatttgt tcaatggttt
      481 gcctcaaaaa atagaggcag gaagatatca ttcatggatt gttgaaaaag aaacattgcc
      541 ttcatgtttg gaagtgactg ctgtggataa tcaaggaatg attatggcga taaagcacaa
      601 aatttatgat gtgcgaggtt tacagtttca tccggaatct gttttaacac cactgggaga
      661 aaaaattctg aaaaattggt tacaaaatac acattaattt tattgattta tcaaaaatta
      721 ttctaaaatg aaagagatac tgaacagatt atttgatcat caatcgttga cacgagaaga
      781 agctgccgaa gtaatgagta atatcggagc agggaaatac aatgaatcgc aaatggctgc
      841 ttttctttcc gtttatctga tgcgaagcat tgagctggac gaattgattg gttttcgaga
      901 tgctttaatg acaatggcaa ttccgatgga tttgtctgaa tttgatccga ttgatattgt
      961 cggaacaggt ggagatggaa aaaatacttt taatatttct acttgtagtt gctttaccct
     1021 tgcaggagca ggttataaag tagcaaaaca tggtaattat ggagccacat caataagtgg
     1081 tgcttccaat gtaattgagt attatggagc aaaatttact acggatattg acattgctaa
     1141 gcgttcgctg gaagagtctg gttttgctta tttgcatgct ccattgtgca atcctgccat
     1201 gaaaaatgta gctcctgttc gcaaaaatat cggtgttcgg acttttttca atgtacttgg
     1261 tcctttaatt agtcctgttc gccctcgtta tcaatgtttg ggtgtgtata atttaaaaat
     1321 gatgcgtttg tacaattatc tttatcaaaa attgggtatt caatattcta ttgtttatac
     1381 ctttgatggt tatgatgaaa tttctttgac agacaaatgc aaaattatta acagtgaggg
     1441 agaatttatt tacacacctg agcaattggg ttttgaaaaa gtaaatcctg cagaattgtg
     1501 gggaggtgaa acagtagaac atgcagcaaa aatctttatg aacattcttg acaatcaatc
     1561 tactgatgga caacgcaatg tagtgattat taactcagct tttgccatta aaacgctttg
     1621 tcctgaaaag gccattgaag aatgtaaggc agaagccatt gaatcacttg caagtggaaa
     1681 agccagagaa gcctttatga agtttgaaga aatttatcat ctttagtttt gtaaaatgga
     1741 agaaaaaaac gagcaaaaaa actcaactat tcaaaaaaca gattttcttg aaaatatttt
     1801 aagaaataaa agagaagaag ttgtgttaaa aaagcaatct tttaaaggaa aaaattggga
     1861 aaattttcct ttcttttcta gaaaatgttt ttctttaaag caaaatttac tttctttgcc
     1921 aataggaatt attgcagaat tcaaacgaaa atctccttcc aaaggttggt taaatcaaca
     1981 ggctgattta tttaaagtta caacagatta tcaacaggct ggtgtagcag gaatttccat
     2041 tttgacagat accgaatttt ttggcggaag cgttgaagat gtgataacag ttcgtgataa
     2101 aatttcctgt cctattttaa gaaaagattt tgtggtnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnntttt attaaagaaa aaaatccagc agatgctcta cgacagttta
     2281 tcaatcaact aattggataa aaattaaaat tggaattttt ttgaaatgaa agataatgaa
     2341 aaaggtagag aagaaaacaa tgaagtaagt caaatagcga atttcaaatt aatcaaagtt
     2401 tgtggaatga agtttccaga aaatattcgt gaagtagctg ctttacaacc tgattttatg
     2461 ggatttgttt tttataagaa atctccacga tatgtaagac atctggatat ttctgtgttg
     2521 gatagtttac cttcgtcgat aaaaaaaatg ggtgtttttg tcaatgaaaa gctggataat
     2581 attttgtcaa cagtttacaa gtatcatctg gacggtgttc aattgcatgg cagcgaactg
     2641 gaagataaat gtgaaaaatt aaaagaagct ggtttgttgg tgattaaagc ttttcctgtg
     2701 gaaagttcgt acaactttat tgtgacaaaa ccttacgaaa gtgtttgcga ttatttcctc
     2761 tttgatacca aagttcccat ttatggtggt gcagggataa aattcgactg gtcgttattg
     2821 aacgattata aaggcaatac tccatttttt ctcagcggtg gcatttcatt ggaagatgta
     2881 gaagatttga aaaaaattga acatcccaag tttattggtg tagatttgaa tagtaaattt
     2941 gaaatagcgc cgggcaaaaa aaatgtcgaa ttgctcaggc aattttttca actttatcgt
     3001 tatagttaaa aaattaatta ttagttaaaa tgaatagaat tcagcaattg tttcagcaga
     3061 aaaaagaaaa tattctttcg gtttatttta ccgcaggttt tcctaattta aatgatacgg
     3121 ttcctgtttt agaaagtttg cagagaaaag gaattgattt ggtagaaatt ggcattccat
     3181 tttccgatcc attggctgat ggacctgtta ttcagaatag tagccaaatt gctttgcgaa
     3241 atgggatgtc attacgaaag ctttttgaac agcttacaga tgttcgctcc aaaattgata
     3301 ttccattgat tatgatgggt tatttgaatc ctatcatgca atttggtttt gaaaattttt
     3361 gtcaggaatg ccatagagtt ggagtggacg gcatgattat tcctgatctc ccaatggatg
     3421 attatttgac tgaatataag gatattgctc agaaatatgg attggaattt gtctttttga
     3481 ttacaccaga gacttccgaa aaacgcattc gtcagattga tgaacacacc aatggtttta
     3541 tttacatggt ttcttcacct ggcgtgacag gcactcgaaa ttcattcgat gaccatattg
     3601 attatttcaa tcggattaat tcaatgcatt taaaaaatcc acgactcatt ggctttggca
     3661 tttccaataa agcaacgcgt gaaactgcct gtcagtattc ttcgggtgtc attatcggta
     3721 gtgctttcat caaagctttg caggaaacaa atgatattga tcaagctgtc gatcttttac
     3781 tggaaagatt gaataaataa atgctgaata nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn aaataaattt ttaaaaagaa aaaaaatccc aatttctttg tgtcaatata
     3961 gtttgacggt taaagtacaa caatcttttt actttttttg tatttctatt cagacaaatc
     4021 tactaatttt gtgtcgcatt caaatttcag aataattttt atgcaacttc caactgcttt
     4081 tgaagaaagg accagattgc ttttgggtga agaatatcca gcattcataa aagcactcaa
     4141 cgaaacgcca cccaccagca ttcgtgtaaa cgataaaatg aattatgtcc cttcttctca
     4201 aaaagtggca tggtgcgaaa gtggttatta tttgcctgaa cgaccttcat ttacaagtga
     4261 tcctcttttt catgcgggag tttattatgt gcaggaagcc agttccatgt ttttgcagca
     4321 agctattaat cagcatttcc ctgaagctaa aaacattctg gatgtttgtg ctgcgcccgg
     4381 tggaaaaacc actttgcttt ctcaatcttt tgataatgat acgttgattg tcagtaatga
     4441 aatcattcat tccagagtaa aaattcttgc tgaaaatgtc atcaaatggg gaaatgccaa
     4501 tgtaattgtt accaacaatg aacctgctga tttcagcaaa ctctcaagtt tttttgatac
     4561 tgtaatagta gatgctcctt gctctggcga aggcttgttc cgaaaaaatc ctgcagctat
     4621 tcaggaatgg tctgttgaaa atgttcaaaa atgtgcagtt aggcaaaaaa aaattgttca
     4681 ggaggtatgg gatgctttaa aaacagacgg aattttggtt tacagtactt gcacatataa
     4741 tcgagaagaa aatgaagaaa atgttttctg gatttgtaaa gaattgggag ccgaattatt
     4801 aaatcttgat gtttctcgct ggaaaaatat aacagtcagt gatggtggtt atcgctttta
     4861 tcctcacaaa attcgtggtg aaggattttt cctttctatt ttgagaaaaa ctgcaaaaac
     4921 gcccataagg aattattcat ttaaaaagaa agaaacttca aaaacgcaac caataagttt
     4981 accctcaatt tctttaaaac gtcctgaaaa ttggatcttt gaactaaata atcagcaatt
     5041 aaaagcttat ccaaaaaaca gatggaaaga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn taaaaaatct gggtaatcgt tacaataatt tgtacccaaa cgaatggcgt
     5221 attagaatga aaatcaatga ataaaataaa atatttttca cgtaagaatt tttgctgaaa
     5281 aaattattaa aatattcact ttttaatcat taaatttgac agaggaaaga acagagatag
     5341 atatatgaaa ttactatttt taggaacggg aacttcaacg ggaattcccc aaattggatg
     5401 ccaatgtaaa acatgtcaat ccaccgatag aaaagacaaa agattaagag cttcgctact
     5461 tgtaaatgtg cagaaaacca atattttaat tgatgcggga ccggatttaa gagaacaatt
     5521 attaacttat caaattagca aactaagcgg aatattgctc acacacgaac attacgacca
     5581 tatcggggga attgatgatt tacgtccatt gggagaatct catttgtatg ccgaaaaaag
     5641 agttttaaat attattcaaa aaaatatgcc ttattgtttt ggtgacaatc tttatccggg
     5701 ggttccgatg ttgcaattgc atgagatcac agaaaaatca aaattctata ttgatcaggt
     5761 tgaaattcag gcagtgagag ttatgcatgc caaattgcct attttaggat ttcggatagg
     5821 taacattgct tatcttacag atgtaaaaac tttagacaat aaagcaattg aacagcttca
     5881 atatttagat ttgctcgtga tcaatgctct cagacagttt cctcatatat ctcatttatc
     5941 gttgcaagaa gccattgagc tgtctgaaaa aatcaacgcc aaagaaactt attttacgca
     6001 tatcagccac gacatgggaa aacatgctga tgtggaagct catttgccat ctcatattca
     6061 tcttgcttat gatggattag aaattgaaat aaactaactt aactttaata aaaatgaaaa
     6121 agtgtagtta cctgattgtt attttcataa atatcttgct aagctcttgt tccagcttgt
     6181 tatttactac gctggacgta ttacgacctg ctcaggtagc ttttccacca gaagtaaaca
     6241 atttattaat tgtaaataat actccccaag attctattca accatcaaaa aaatcattta
     6301 ccaaagatag cctttcaatt tattgtttaa gtgctctcac agaagaattg ataaatcgag
     6361 gtttttttca gaatgttttg ctggaattaa attcccttaa taaaaccaaa aatgatacgt
     6421 ctatttatta tttgacacca gaagtagtaa cccaactttg ttccaattat aaaagtgatg
     6481 caattcttgc tttaaacaaa ctgaaagaaa cagatatttt aacagaatat tttgtagaag
     6541 atgagaattc ttttctttcc acttttgaag tttttataga aacctactgg agtattcatt
     6601 atcctgataa gacagatttt acagtcttac aatttaaaga ttcgatgtat tgggaatcag
     6661 aaaattattc acgtcaagct gctttagctc aacttccaaa taaaattgat gctcaaattg
     6721 atgcagcttt atatgcagga agaaattgtg taaaacgttt cgtaccttac tgggaaaaag
     6781 tagatcggta ctttttcaat cctcgaaaca aattgatgcg tcaggctatg gattctgttt
     6841 attataaaaa ttgggaagct gccattaaat tatgggataa agcgattcaa acaaccaaaa
     6901 acacaaaatt acaagctcaa gcagccaata atatagctat agcttatgaa atttttggcg
     6961 atttggataa agctattgaa tatgctaccc tttcgctaaa atcattttta aatgtaacga
     7021 cagtagataa ggaaactatt atcagaattt caaattatcg aaatgaattg atcaaaagaa
     7081 aagaggaaat cgaactatta gaaattcaat taggcgaata atgctttgtt aaagaatata
     7141 agatatctta ttttgtaata taacattcca aaaaatagtg tatgattcac ttgtcacctt
     7201 ttgatgaaat cactttacga gaaaaaggat taaccatatc ccagattgaa gaacagctcc
     7261 gcttttttga aacgggtttt cctttcttag aaattgtacg acctgcaaca gtagatgacg
     7321 gaattttgaa aatccaagaa aacgagaaga aaaaattaat agcattgtgg gataaatatc
     7381 tgtaccagag caaaatagaa ataatgaaat ttgttcccgc atcgggtgct gccagcagaa
     7441 tgttcaagga tttgtatgaa tttttggaaa gtaaagagga aattccttca aatggagtta
     7501 ttcgtcaatt cttttctgaa attgataaat ttgctttcta tgacctatta aatgaaactt
     7561 gtgtaaaaaa tacaggtaaa aatattcaaa tgctaataca agaaaaaaaa tacaaaatca
     7621 ttattgaaaa tttattaaat gaaaggggat taaattatgg ctctttgcca aaagggttgt
     7681 tgttatttca tacttatcct gacgaaaaaa gaactccagt tcaagaacat ttagtagaag
     7741 gtgcacttta cacagttaat gctcaaaaag aagtaaacat tcactttact gtttctcctc
     7801 aacacaaagc cttattcgaa aaacatatta gtgaagtaaa ggaaaaatat gagaaaaaat
     7861 ttaaagttca ttattccgta agtttttccg aacaaaaacc cagtaccgat accattgctg
     7921 tggacatgaa caatcaaccg attcgtgaaa atggcaaact tgttttcaga ccgggtggac
     7981 acggggcttt gattgaaaat ttgaatgatc tcaatagtga tattattttt ataaaaaaca
     8041 ttgacaatgt agtacctgat gctttgaaag gaccaactgt aacttacaaa aaaattctgg
     8101 ctggacttct aaccaatgtt caacatcaat gttttcaata tttaaacgag cttgaaaata
     8161 aaaacatttc agaaagaaaa ttaaatgaaa ttttacaatt tactgaaaag accttaaata
     8221 tttatcatcc taatatttct gcattcgata tttgttctct tagaaattat ttaattacca
     8281 aactgaatcg ccctttacga gtttgcggca tggtaaaaaa tgtaggagag ccaggaggcg
     8341 gacctttttt tatcgtcaat gcggatggca ccatttctct tcaaatttta gaaagcgcac
     8401 aaattaatac acaaaatcct gctgataaag aaaaaatgga acgttctacc cattttaatc
     8461 ctgtggattt ggtatgtgct gtaaaaaact ataaaggaga aaaatttaat ctgttaaact
     8521 atgtagataa aaaggcaggg tttatttcta ttaaaacaaa aaatggaaaa aaattaaagg
     8581 cactggaact tccagggtta tggaatggag caatgagtga ctggaataca atttttgtag
     8641 aagt
//
LOCUS       DAOSAS010000076         8447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_5477_length_8447_cov_11.855338, whole genome shotgun sequence.
ACCESSION   DAOSAS010000076 DAOSAS010000000
VERSION     DAOSAS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5477_length_8447_cov_11.855338"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(707..1210)
                     /locus_tag="PLR72_04710"
     CDS             complement(707..1210)
                     /locus_tag="PLR72_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36538.1"
                     /translation="MATGAVVGALNHGAHEMANKVAFFKRLREHYESGTKEDFKLTSR
                     EFAYLIRSGKINVSSSIKIGDNEYACTIDFYNSNNDLRFSFGRSTVTYYVKDGVRIYN
                     GFYDTYDFDSKPWGTRSPINEIITRTYDSISSGSSFEIFYNQSMLFQQEGFIQNIWLR
                     GNSIKLP"
     gene            complement(1378..1806)
                     /locus_tag="PLR72_04715"
     CDS             complement(1378..1806)
                     /locus_tag="PLR72_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36539.1"
                     /translation="MNSNFKKIIYLLISIGIIITLIYFIYKSCFERTPASIVKKVFDV
                     EVKKKNKLNVFKEQWDYNGNGYCLIIFNNVDSDDITMKYKVEKLPLRIELPPTEITSI
                     LSNIQRGEYWVKFDSNDSRNLYLFVYDKIKRRAIFYYQLF"
     gene            complement(1854..2930)
                     /locus_tag="PLR72_04720"
     CDS             complement(1854..2930)
                     /locus_tag="PLR72_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOK36540.1"
                     /translation="MVKNGTLYYVHTDNLVSIQAITDENKNIVSSYYYTPWGGRVLLS
                     GANITDRGYTFHEHLEPFGLINMNGRVYDPLTAQFFSPDPVLQAPGDWLNYNRYGYCM
                     GDPLKYTDPSGYVSDFYRWAHDNRKMVATTGAAIVVGAGIFVLTGGTGSVVAVQVAAG
                     MIAATASSITSSVLGSALNGGSFGDCFSSGIKGGIISGATSALGGALSIVGGAGMTFA
                     ENLLLGTAEGAITDGFSALLSGTDIGTGMLVGAAAGAILTTVFSENTSNLFKGKGFYT
                     NENVFNHMIADGVRIYNGFYDTYDFDSKPWGTRSPINEIITRTYDSISSGSSFEIFYN
                     QSMLFQQEGFIQNIWLRGNSIKLP"
     gene            complement(3456..4223)
                     /locus_tag="PLR72_04725"
     CDS             complement(3456..4223)
                     /locus_tag="PLR72_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36541.1"
                     /translation="MRTKYLVLMGFGLFFCNSIYSQGIVYKLKQFYQNADMDCYIAKI
                     QKNVNDYFVLDNKIWGRDKHGIPPQKIELHKEEIVPMLTIQFINTDSFNLNDDIYNYI
                     AIDSSIVFTLACIDKKMNVTAFAHFFEWASGYYEIDKDPSIKNSHERMKFKHIIKNIN
                     KQEPEMILYCNNLCGCVGLCGPCDNNGFMYVKDGKIYVYRVIEKDIYELNDYIRKFFS
                     LERIRKLNYSYIPIIYQLDQSPRRTGHALENEKIICP"
     gene            complement(4233..5465)
                     /locus_tag="PLR72_04730"
     CDS             complement(4233..5465)
                     /locus_tag="PLR72_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOK36542.1"
                     /translation="MVKNGTLYYVHTDNLGSIQAITDENKNIVSSYYYTPWGGRVLLS
                     GANITDRGYTFHEHLEPFGLINMNGRVYDPLTAQFFSPDPVLQAPGDWLNYNRYGYCM
                     GNPFKYTDPTGEVFVVDDAIIILVMAYFGGMQANFFTAKNPMNPADWNWSSPSTYIGI
                     GSGVLAGVSMAGYQVPTLFPNLDGIVTRGVYQAGVNVGLNGIANTINGDSFFDSWGGA
                     ALSGFINGAITGYQLADVNGKNLWWGSEIGYNRNKWSFFNWDVPDYLIDFGMPNVGSN
                     LDNDCVPTTFAEIEAKRGGSRTYDDFKGISNYQENVGVATTQKGYETLVNKTFSNAQT
                     LSRTDYHNLFDANYMQNAANNNEVFSVHFRGHADNVRSLRVFTRAPYKNTLTFRQGSY
                     NFTSSGQGVVIWNIFRFF"
     gene            complement(6077..6454)
                     /locus_tag="PLR72_04735"
     CDS             complement(6077..6454)
                     /locus_tag="PLR72_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36543.1"
                     /translation="MRNSIYFISALVVLSCSVSGQSKYIIENREMLKDYFLYSCIMHG
                     FENSDIKKKDHSGAVYIDLLRYDLKALHKTDSIAKAFVSSIETSPYENRNTKGIIILS
                     IEEYKSKRIDEFIKSMDIYMLKE"
     gene            complement(6465..7802)
                     /locus_tag="PLR72_04740"
     CDS             complement(6465..7802)
                     /locus_tag="PLR72_04740"
                     /inference="COORDINATES: protein motif:HMM:NF025478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector amidase Tae4 family protein"
                     /protein_id="HOK36544.1"
                     /translation="MVKNGTLYYVHTDNLGSIQAITDENKNIVSSYYYTPWGGRVLLS
                     GANITDRGYTFHEHLEPFGLINMNGRVCDPVLARFLSPDNYVQAPDFTQSFNRYSYCL
                     NNPLKYTDPSGEFIFSLFLPGIGTLLDAACWGAVIGGAGYTASIAFSDGGFNNWNSGD
                     FWKSVGVGAISGAVTGGVGQMFGPVGSMGFGGEIARAYTHGFAQGIVSEFTGGDFMTG
                     FAAGGLSSLAGSSFMMYGGRFASSTLGNYAFSGLAGGIGAELTGCNFWQGAATGLMTA
                     GLNHLQQGLTRPKFDDLLANYPTDGQGGDMSADEVYKLIGGDVLAEHNRDPNAYTNAC
                     ALRTSRALNYAGTDIPNIPGQTLTGADGKNYFYRASDLYKWMSKPAVFGKPNISTSDF
                     SKFTGNRGVFIMQAHYPAQFGAWGHATLYNMTGCMGGHCYNGPYAYRYNLWKF"
     gene            complement(8169..>8447)
                     /locus_tag="PLR72_04745"
     CDS             complement(8169..>8447)
                     /locus_tag="PLR72_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36545.1"
                     /translation="SKNPTTQPGNKTLYLRITQPDEEVLIKSPDDLFPYEDKQIAYSV
                     KKDFEYSGEAISDVIYWKVDRVLQIGTYRADFFVDGFRIGSFTFEIEK"
BASE COUNT         2630 a         1698 c         1378 g         2741 t
ORIGIN      
        1 attttccggc tcttttatac ttatttatca aaaaaatcaa aaagtaattt actttcaatt
       61 tcaaaagtgt ttactattgt aatttagcaa catacaaatt tatgatacaa atatgaagca
      121 aatttaaggt aaataacaat aagatttaga gaaatgattt acaaaaatat gaatgatttt
      181 tcaataagta atgataagga caataaaaaa acaaaatagt ttcaaaatat tgtttctgtt
      241 ttagaaatta gatttttttt gatttgatag caagataaag accaacaagc acaacaagga
      301 taagggctat tcgtccgcac cagatttgaa agttttgcca gcttgaaagg gaggctttga
      361 ctggtttaac aacttccacc ggaaagggaa tgcggatagt gtcgtttagg tagattgaat
      421 ctttaagaat tttgtttttg taaagatata tgtaatgttc caaaatcagg gtgtcgcctt
      481 tatcttttac atatatagaa tcgtaatgaa aaattgagtc tttgacaaaa ttatcccggt
      541 actctatttt tgtagtttct atgggaatgt acttggtagt tttacaggaa gtgcaagtaa
      601 ctataaatgt tgcaataacg gtatataaaa taaattattt tcttgaaatt cgagttcata
      661 ttagttaatt tttcaattta aatcttgctt acaataaatt tataatttat ggcaatttta
      721 ttgaattacc tctcagccat atattttgaa taaaaccttc ttgttggaat aacatacttt
      781 gattataaaa tatttcaaaa gaactaccac ttgatattga atcatacgtt ctggttatta
      841 tttcattaat aggagatcta gttccccatg gttttgaatc aaaatcataa gtatcataaa
      901 acccattata tatccttaca ccatctttca cataatatgt tacagtactt cttccaaacg
      961 aaaagcgtaa atcattattt gagttataaa aatcaatagt acaagcatat tcgttatccc
     1021 caatttttat ggaactactt acgttaattt ttccgcttct tattaaatat gcaaattctc
     1081 tacttgttag cttaaaatct tcctttgtgc cactttcata atgttccctc aatctcttaa
     1141 aaaatgctac tttatttgcc atttcatgcg ccccatgatt caaagcccca accactgcac
     1201 cggtagccat accaaaaaga atttcttcag gactttctgg cgccggcaat cacaaatcct
     1261 acgcctccca ttacagtaga tactcctaca attcctgcaa agtctgtaat ccctgcagct
     1321 ccagaaaatg aaccgccaaa agaaccaaga gcgcccgccg cggctcactg gttcaaatta
     1381 gaatagctga taataaaaga tagctcttct tttgatttta tcataaacaa acaaatataa
     1441 atttcgcgaa tcatttgagt caaatttaac ccaatattcc cctctttgga tattacttaa
     1501 tattgaagta atttctgttg gagggagttc gattcttaat ggtaattttt caaccttata
     1561 tttcatagta atatcatcgc tatctacatt gttaaatata attaagcaat aaccgtttcc
     1621 attataatcc cattgttctt taaaaacatt taatttattt ttcttcttta cttcaacatc
     1681 aaaaaccttc ttcactatac ttgctggagt tctttcaaaa caagatttat aaataaaata
     1741 aatgagagta ataattattc cgattgagat taataaataa attattttct tgaaattcga
     1801 gttcatatta gttaattttt caatttaaat cttgcttaca ataaatttat aatttatggc
     1861 aattttattg aattacctct cagccatata ttttgaataa aaccttcttg ttggaataac
     1921 atactttgat tataaaatat ttcaaaagaa ctaccacttg atattgaatc atacgttctg
     1981 gttattattt cattaatagg agatctagtt ccccatggtt ttgaatcaaa atcataagta
     2041 tcataaaacc cattatatat ccttacacca tctgcaatca tatgattaaa cacattttca
     2101 ttagtgtaaa aacctttgcc tttaaataga ttgcttgtat tttctgaaaa caccgttgtt
     2161 aaaatggctc ccgcagcagc acctactaac attcctgtgc ctatatctgt acctgagagt
     2221 aaagctgaga aaccatcagt tatggcacct tcagctgttc ctaataatag attttcggca
     2281 aatgtcattc cagctcctcc tacaatactt aaagctccac ccaaagcact tgtagcacca
     2341 ctaattattc cgccttttat tccggatgaa aagcaatcac caaaactgcc tccattcaga
     2401 gctgatccta acacactact agtaatactc gaagctgtag cagctatcat tcctgcagct
     2461 acttgtactg caactacgct tccagttcct ccggtcaata caaatattcc tgcacccacc
     2521 acaattgcag ctcctgtagt ggctaccatt ttcctgttat catgagccca tctgtaaaaa
     2581 tccgatacat aaccgctcgg gtcggtatat ttgagcggat cccccataca atacccatat
     2641 cggttatagt ttagccaatc accaggagcc tgtaaaacag gatcaggcga aaagaactga
     2701 gctgtcagcg gatcatacac acgaccgttc atgttgatca gcccgaatgg ttccaaatgt
     2761 tcgtgaaacg tatagcctct gtcggtgata tttgctccgc tgagcaatac cctgccaccc
     2821 catggggtgt aataataact gctgacgatg tttttatttt cgtctgttat ggcttgaatg
     2881 gagactaaat tgtcggtatg aacgtagtac aatgttccat tcttcaccat tgcagttaat
     2941 cctgccgggg tgtaaatata atcggtttgg gtgaactttc caaatttatc tatgtctttt
     3001 tcgtaagtgc caaagtaata gcgaatgtta ttatattagg gggaaagttt ggttttatga
     3061 gcggttaaaa tcaagagatt tttttactta cgacgtaggc tggaggctac gccgaacgat
     3121 aaggtaaaat atactgaaaa aatggacgtt tgttggctaa aatctttatc gttctattgg
     3181 atttgcaatc tgacaggatt atttgcggat tttaaatccg ctattcactt accaaatgat
     3241 tacaaatcct ttgtgacaga gtaaatggct agctgtaatt tttcggattg cggcggttgc
     3301 cctttctcaa aaaacgcctg gctacaccca aaagtaaacc attttttggt gcgaagcaac
     3361 ccgatggcga tttttttagg aaggctacag gaaaatagca gcgctgggcg gcgcaacagc
     3421 tggcaagctg aaatacccat ccattccaac ttgaattatg ggcatataat cttttcgttt
     3481 tcaagagcat gccctgtacg ccggggtgat tgatcaagtt gataaataat tggtatataa
     3541 gaataattta acttgcggat tctctccaaa ctaaaaaatt tccgtatata gtcgttcaat
     3601 tcataaatat ctttttcaat tacgcgataa acatatatct taccatcttt tacatacata
     3661 aacccattat tatcacatgg cccacacaga cctacacatc cacacagatt attacaataa
     3721 agaatcattt caggttcttg cttattaata ttctttatta tatgcttaaa tttcatcctt
     3781 tcgtgggaat ttttaattga aggatcttta tcaatctcat aatagccact ggcccactca
     3841 aaaaaatgag caaacgcagt aacattcatt ttcttatcaa tacaagcaag agtaaataca
     3901 atagagctat caatagctat gtaattgtaa atgtcgtcgt tgagattgaa tgaatcagta
     3961 tttataaatt gaatggttaa cattgggact atttcttctt tgtgcaattc aattttttga
     4021 ggagggatgc catgtttgtc acgtccccaa atcttattat ctaaaacaaa atagtcattt
     4081 acattttttt gaattttagc aatataacaa tccatatctg cattttgata gaattgtttt
     4141 aacttatata caataccttg cgaataaata ctattgcaaa aaaacaatcc aaatcccatt
     4201 aatactaagt attttgttct cataaatgtt tattagaaaa accggaaaat attccaaatc
     4261 actacacctt gaccagatga tgtaaaatta taactgcctt gccggaatgt aagagtattt
     4321 ttatatggtg cacgtgtaaa cactcttaga cttctaacgt tatcggcatg acctctaaaa
     4381 tggactgaaa acacctcgtt gttgtttgca gcattttgca tataatttgc atcaaataaa
     4441 ttatgatagt cagttcttga aagtgtttgt gcgttactaa atgttttatt tactaatgtc
     4501 tcataacctt tttgcgttgt tgctacacct acattttcct gataattaga aattcctttg
     4561 aaatcatcat aagttcttga ccctcctctt ttagcttcaa tttcagcaaa tgtagttggt
     4621 acacaatcat tatctaaatt tgatcccacg ttaggcatgc caaaatcaat caaataatca
     4681 ggaacatccc agttgaaaaa cgaccatttg tttctattat acccaatttc tgatccccac
     4741 cataaattct tcccattaac atccgccagc tggtaacctg taattgcccc atttataaaa
     4801 cccgatagcg ctgctcctcc ccaactgtcg aaaaacgagt cgccattaat cgtgttggca
     4861 atgccattta accctacatt caccccggct tggtatacgc ctcttgtaac tatgccatcc
     4921 aaattcggga aaagtgtagg tacctgatag cctgccatgc ttacgcctgc caacactcct
     4981 gacccaatgc ctatatatgt acttggactg ctccagttcc aatccgcagg gttcatcggg
     5041 tttttggcag taaagaagtt tgcctgcata ccgccgaaat atgccataac taagattatg
     5101 attgcatcgt ccacgacaaa tacttctccc gtagggtcag tatatttgaa cggattcccc
     5161 atacaatacc catatcggtt atagtttagc caatcaccag gagcctgtaa aacaggatca
     5221 ggcgaaaaga actgagctgt cagcggatca tacacacgac cgttcatgtt gatcagcccg
     5281 aatggttcca aatgttcgtg aaacgtatag cctctgtcgg tgatatttgc tccgctgagc
     5341 aataccctgc caccccatgg ggtgtaataa taactgctga cgatgttttt attttcgtct
     5401 gttatggctt gaatggagcc taaattgtcg gtatgaacgt agtacagtgt tccattcttc
     5461 accattgcag ttcattctgc cggggtgtaa atataatcgg tttgggtggt gtttccaaat
     5521 ttatccatgt ctttttcata agtgccaaaa taatagcgga tgttattttt gctcggcgta
     5581 atgtttggtt ttatgagcgg ttaaaatctg gagattttgg cacaggtaaa tggctgacgc
     5641 cagtgagaaa atttggaaat ttgatttttt atgcgactta gtctggattc tacaccagac
     5701 aaagatatga ggctttgtcc caaaccccac ttactttttt atcttgacat aaaaaagtaa
     5761 gcaaaaaagt taacttcgtt gaagcagcta cgctcaacat aattcaaata aatttgattc
     5821 tgttttcgct taaacgctgc tttcaagact ctacccgctt cgcacgaaaa atggacgttt
     5881 gttggctaaa aattaaccgt tatgtcggat ttgcaatctg acagaattat ttgcggattt
     5941 taaatccgct attcacttac caaaggatta caaatccttt gggacagagg actcgtgcgg
     6001 tagcgaggag ttcctggacc tttataaaca atataggtaa attaagcccc ggaactctcc
     6061 gggacttttc tttaaattat tctttcaaca tataaatatc catcgatttt ataaactcat
     6121 ctatcctttt gcttttatat tcttcaatgc taaggattat aatgccttta gtattcctat
     6181 tttcatatgg tgaagtttca atagagctga caaaagcttt tgcaatacta tctgttttat
     6241 gcaatgcttt taaatcatat ctcaataaat caatataaac agctcctgaa tgatcctttt
     6301 tctttatgtc cgaattttca aatccatgca tgatacatga atataaaaaa taatccttta
     6361 acatttccct attctcaata atgtatttgg attgtccact aaccgaacaa ctaagaacaa
     6421 ctaaggctga aataaaatat atcgaatttc tcataagcat atgattaaaa tttccaaaga
     6481 ttgtatctgt atgcatacgg accattataa cagtgacctc ccatacaacc tgtcatgtta
     6541 tataaagtag catgccccca tgctccaaac tgggctggat aatgtgcctg cattataaat
     6601 actccacgat tgcctgtaaa ttttgaaaaa tctgaagtag agatattggg tttgccaaaa
     6661 acagcaggtt tcgacatcca cttatataaa tcagaagctc tataaaaata atttttacca
     6721 tctgcccctg ttaatgtttg tccaggaatg ttaggaatat ccgttcctgc ataatttaaa
     6781 gctcttgaag ttctcaaggc acatgcgttt gtataagcat taggatctct attatgttcc
     6841 gctagcacat ctccacctat taacttatag acttcatcag cagacatatc acctccttgc
     6901 ccatcggttg gatagttagc taataaatca tcaaatttgg gtcttgtcag tccctgctgt
     6961 aaatggttta atccagcagt cattaagcct gtagccgcac cttgccaaaa attacatccc
     7021 gttaattctg cacctattcc tccagctaat ccagaaaaag cgtaatttcc taaagtacta
     7081 cttgcaaaac gtccgccata catcataaaa gaagaacctg caagtgaact taaaccgcca
     7141 gcagcaaaac cagtcataaa gtcaccgcct gtaaattcag atactatacc ttgtgcaaaa
     7201 ccatgtgtgt atgccctggc tatttctcct ccaaatccca tgcttccaac tgggccaaac
     7261 atttgaccaa ctccgccagt aactgctcct gaaatagcac caacgccaac cgacttccaa
     7321 aaatccccgc tattccaatt attaaaacct ccatcgctaa aagcaatact tgccgtatat
     7381 ccagctccac caataactgc accccagcag gcggcatcaa gcaaagttcc tatacctggt
     7441 aaaaagagag aaaatatgaa ctccccgctc ggatccgtat actttaatgg gttattcagg
     7501 cagtagctgt agcggttgaa actctgggtg aagtcgggtg cttgcacgta attgtctggg
     7561 ctgaggaagc gtgccagcac gggatcgcat actcgcccgt tcatgttgat cagcccgaac
     7621 ggttccaaat gttcgtgaaa cgtatagcct ctgtcggtga tatttgctcc gctgagcaat
     7681 accctgccac cccatggggt gtaataataa ctgctgacga tgtttttatt ttcgtctgtt
     7741 atggcttgaa tggagcctaa attgtcggta tgaacgtagt acagtgttcc attcttcacc
     7801 attgcagtta atcctgccgg ggtgtaaata taatcggttt gggtgaaatt tccaaattta
     7861 tctatgtctt tttcataagt gccaaaataa tagcggatgt tatttttgct cggcgaaatg
     7921 tttggtttta tgagcggtta aaatcaggag attttggcag tggtaaatgg ctgacgccag
     7981 tgagaaaatc tggagatttt ttacttacga cgtagcctgg aggctacgcc gaacaaggaa
     8041 aatctggaga atttttttac ttatgacgta gtctggaggc tacgccggac acaaggctac
     8101 gccgaacgat aaggtaaaaa tatatacagt aaaaaaacaa tatttttttt aatctttatt
     8161 taggtaattt acttttcaat ttcaaaagtg aaacttccga tcctgaagcc atcaacaaaa
     8221 aaatcagcac gatacgttcc aatttgtagg acgcgatcta ctttccaata aataacgtca
     8281 cttatagctt cacctgaata ttcaaaatct ttctttacag aataggcaat ttgtttatct
     8341 tcgtatggga aaaggtcatc aggacttttt atcaatacct cttcatctgg ctgagtgatt
     8401 cgtaaataca aggttttgtt tccaggttga gttgtaggat ttttact
//
LOCUS       DAOSAS010000077         8430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_5501_length_8430_cov_13.430209, whole genome shotgun sequence.
ACCESSION   DAOSAS010000077 DAOSAS010000000
VERSION     DAOSAS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5501_length_8430_cov_13.430209"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PLR72_04750"
     CDS             <1..698
                     /locus_tag="PLR72_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3372 domain-containing protein"
                     /protein_id="HOK36546.1"
                     /translation="SVKFGIVGAIFHPQVDYTQLIYSKEPYAKDPIQVINYVSCHDDL
                     CLVDKLKKSLPKDASEEELIKFDKLAQTVVFTSQGIPFIYAGEEVFRNKKGVHNTYQL
                     PDSINQINWNYKTVYHDIFEYYKGLISLRKLHPAFRLATEQSVQEHLKFIDMNTPNVI
                     AYQLSQHANGDNWREILVVFNGNRKPVEVEIPGGEWNLVCYDGKINLAGISVVNNLNL
                     VVPASSASILYQN"
     gene            722..2722
                     /locus_tag="PLR72_04755"
     CDS             722..2722
                     /locus_tag="PLR72_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOK36547.1"
                     /translation="MEEERFSGLPENAHRELKDGEEYHPILSPEKNYPEVNFRSVSVG
                     LLMAILFSAAAAYLGLKVGQVFEAAIPIAIIAVGLSSAAKRKNSLGENVIIQSIGASS
                     GVIVAGAIFTLPALYILQAKYPEITVSFMQVFLSSLLGGVLGILFLIPFRKYFVADMH
                     GKYPFPEATATTQVLVSGEKGGDQAKPLIRAGIIGGLYDFLIATFGSWAEVFTSRVLP
                     FGTMLADKIKLVFKVNVGAAVLGLGYIVGLKYAAIICAGSVLVWFVIIPILPLFNDSF
                     LHILNPVFTENLINVPPEIIFRDFARHIGIGGIAMAGIIGVINSWSIIKNAIRLASKE
                     FKKNKPVETTVKRTQRDLPMKIIIVGIVVTLLLTFAYFYFGVVGNLLQAIVGILVIGI
                     ITFLFTTVAANAIAIVGTNPVSGMTLMTLILSSVILVAVGLKGPTGMIASLVMGGVVC
                     TALSMAGGFITDLKIGYWIGTTPAKQETWKFLGTLVSAATVGIVIMILNKTYGFTGEK
                     ALVAPQANAMAAVIEPLMSGGGAPWLLYAFGALIALVLNFLKVPALAFALGMFIPFEL
                     NIPLLIGGGINWLVSTRTKDKKLNQVRQEKGTLLASGFIAGGALMGVVSAALRFGGLD
                     FINNKWAESAAAEWLALIMYAVLIGYFIWDVMRISPKDKQKN"
     gene            2789..4108
                     /gene="ffh"
                     /locus_tag="PLR72_04760"
     CDS             2789..4108
                     /gene="ffh"
                     /locus_tag="PLR72_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOK36548.1"
                     /translation="MFESLTERLERSFKILKGEGIITEINVAETLKDVRRALLDADVN
                     YKIAKTFTDTVKEKALGQDVLKSLKPSQLMIKIVHDELAELMGGTSTDINLKGSPAII
                     LMAGLQGSGKTTFAAKLANFLKTKRGKNPLLVACDVYRPAAIEQLKVLGEQIQVPVYA
                     ELENKNPISIAESAVKYAKLHGYDVLIIDTAGRLAIDEMMMTEISAIKQAIQPQEILF
                     VVDSMTGQDAVNTAKEFNDRLDFDGVVLTKLDGDTRGGAALSIRSVVNKPIKFVSTGE
                     KMDALDVFHPERMADRILGMGDIVSLVERAQEQYNEEEARRLQKKIAKNQFDFNDFLS
                     QIHQIKKMGNFKDLASMIPGVGKALKDVDIDENAFKSIEAIIQSMTPEERENPSLLNG
                     SRKQRIARGSGTTIVEVNRLIKQFDQISKIMRVATVGSNKMKMKKKK"
     gene            complement(4499..5461)
                     /locus_tag="PLR72_04765"
     CDS             complement(4499..5461)
                     /locus_tag="PLR72_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK36549.1"
                     /translation="MNKKICVVGGGKWGQNHIKTLYQLGNLGGIVEANAKRLNELLKI
                     YPVEGFTELEEALEKDFDGFTVAVPAPLHYEVTKKIIESGKSVLVEKPMTLSTETSRE
                     LVELAERKGVQLMAGHVLLFHPAIRKIKEIIDSGELGNLYYFYSNRLNLGTVRTEENV
                     FWSFAPHDISVLDYFVGHPAEKIVSKGSKFLQENVYDVTITQLDYPNNVHAHIFVSWL
                     HPFKEQRLVVIGSKGMISFDDASIDKEIKFYDKRIHFEKGIPVKVENPTRIIPYEQKQ
                     PLTEELKYFIEHLDKKIEIADGRSGLEVVKVLEEAEKKLRIGSQ"
     gene            complement(5464..6771)
                     /locus_tag="PLR72_04770"
     CDS             complement(5464..6771)
                     /locus_tag="PLR72_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOK36550.1"
                     /translation="MITSEDFLQKIESNQIIIGIIGLGYVGLPLATTFAQASIKVIGF
                     DKNPDKVKKINSGENYISDISDEEFCEIISDGKFTATDDFSRIKECDALIICVPTPLD
                     IFQKPDMSFIESACIEIGQNMKLGTFISLESTTFPTTTENFMLPIIERESGLKHGVDF
                     WLAYSPERVDPGNQTYKTKNTPKVLGAINEEGLKIGEALYKKAIQNVHLVSSPRVAEM
                     VKILENTYRLINISLINELALLAGKMDINIWEVIEAAKTKPFGFQAFYPGPGIGGHCI
                     PLDPFYLEYIAKKFDFDLTMIHTAGHINMRMPHHMYNKITSALNRQKKAINGSKIFFL
                     GVAYKRDIDDDRESPALEIIDIIANKGGIVSYNDPYVPAITTPEGYSFQSTEYSAKNL
                     ENADLVVITTNHSIYKADFIMQHAHLIVDLRNVIPPAKNVYKL"
     gene            complement(7012..8112)
                     /locus_tag="PLR72_04775"
     CDS             complement(7012..8112)
                     /locus_tag="PLR72_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK36551.1"
                     /translation="MTKDIKEEKKVKNSVCAIVVTYNRGNLLIECLNALLKQTFSLDA
                     IYIIDNSSTGDTPYILKENHYLTELPPENLKEPWEIDFKVKKSDESKIITIYYVRMNE
                     NTGGAGGFYEGVKRAYEKDYDWLWLMDDDAEPAKDALEKLSRFFDCKNVSALAGSVTF
                     LDGSLNFEHRSYIDLNQNFYAFQQSVEPALYKNPFVEIEMASFVGLLINRKIVQKAGY
                     PKKEFFIHHDDFEYCLRLHQIGRILLIPDSIIVHKEAGKDAGSLKKFLGIKSYRIPYD
                     KLWLTYFSKRNLIWLGKKYSHKRMNYYLILIRDFLKSFTGIIFYDNNKWRRIKFLVNC
                     YLDGLRGNFDNKKPQKILYYEIKNQQAIFQFL"
     gene            complement(8109..>8430)
                     /locus_tag="PLR72_04780"
     CDS             complement(8109..>8430)
                     /locus_tag="PLR72_04780"
                     /inference="COORDINATES: protein motif:HMM:NF015247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HOK36552.1"
                     /translation="YFQEVAVVNYPNDYDFTRITEFKHIHPVETGKTVILKEYPQDYQ
                     WGKNLPFYPILKEKNLELYHRYLELAKKFKNLILVGRLADYQYYDMDDIIKKSLEIFE
                     EKVK"
BASE COUNT         2636 a         1410 c         1497 g         2887 t
ORIGIN      
        1 aaagtgtaaa atttggtatt gttggagcaa tttttcatcc tcaagtggat tatactcaac
       61 tcatttattc aaaagaacca tatgcaaaag atcctattca ggtgattaat tatgtttcct
      121 gccatgatga tttatgttta gtagataaat taaaaaaatc tttaccgaaa gatgctagcg
      181 aagaggaatt aataaaattt gataaattag ctcaaacagt tgtcttcact tcgcagggaa
      241 ttccatttat ttatgcaggt gaagaagttt tccgtaataa aaaaggcgtt cataacactt
      301 accagttgcc cgattctatt aatcaaatca attggaatta caagactgtt tatcacgata
      361 tttttgaata ttataaagga ttgatctcgc ttcgtaagtt acatcctgct tttagattgg
      421 caacagaaca aagtgtacag gaacatttga aatttattga tatgaatacc cctaatgtca
      481 ttgcttatca actttcacag catgctaatg gagacaactg gcgagaaatt ctggttgttt
      541 tcaatggcaa taggaaaccg gtagaagtgg aaattcccgg aggtgaatgg aatttggttt
      601 gttatgatgg aaaaatcaat ctcgcaggga tttcagtagt taacaatctt aatttagtgg
      661 ttcctgcttc gtcagccagc attttatatc aaaactaatc tgttgattta gaataaaata
      721 aatggaagaa gaaagatttt caggattgcc agagaatgct catcgtgaat taaaagatgg
      781 tgaagaatat catcccattc tttcacctga aaaaaattat ccagaagtta attttcgttc
      841 tgtgtctgtt ggtttgctaa tggcaattct tttttcagca gcggcagctt atttaggttt
      901 gaaagttgga caggtgtttg aggctgcaat tcccattgcc attattgctg taggactttc
      961 cagtgctgct aaacgaaaaa attcgctggg cgagaatgtt attattcaat ccatcggtgc
     1021 cagttctggt gtcatagttg caggagcaat ttttactttg cctgctttgt atattcttca
     1081 ggcaaaatat ccggaaatta ccgtaagttt catgcaagtt tttcttagtt cgcttttggg
     1141 tggagtgctg ggaattttgt ttttaattcc tttcagaaaa tattttgtgg ctgatatgca
     1201 tggaaaatat cctttccctg aagcaactgc tactacgcaa gttctggttt ctggtgaaaa
     1261 aggtggtgat caggcaaagc ccttgattcg tgcaggtatt attggaggtt tgtatgattt
     1321 tctcattgct acttttggaa gttgggctga agtttttact tcacgtgttt tgccttttgg
     1381 gacaatgctg gctgataaaa ttaaactcgt ttttaaggta aatgttggtg cagcagttct
     1441 tggcttaggt tatattgttg gtttaaaata tgctgctatt atttgtgcag gttcagttct
     1501 ggtatggttt gttattattc caattcttcc tttattcaat gatagttttt tacacatatt
     1561 gaatcctgtt tttactgaaa atttaattaa tgttcctcct gaaattattt ttcgtgattt
     1621 tgctcgtcat attggcattg gtggaattgc aatggcaggc atcattggtg ttatcaattc
     1681 atggagcatc attaaaaatg cgattcgttt agcttcaaaa gaatttaaaa aaaataagcc
     1741 tgttgaaaca acggtaaaac gcacgcaacg tgatcttccg atgaaaatca ttattgttgg
     1801 aattgttgtt actttactgt tgacttttgc ttatttttat tttggtgtcg taggcaattt
     1861 attgcaggca atagttggca ttttagtcat tggtatcatt acttttttgt ttactacagt
     1921 agccgcaaat gctattgcca ttgtaggaac aaatcctgtt tctggaatga cccttatgac
     1981 tttaatttta tcgtcagtga ttcttgtggc agtaggtttg aagggaccga ccggcatgat
     2041 agcttcactt gttatgggag gcgttgtttg tacagcatta tctatggcag gtggatttat
     2101 taccgattta aaaattggtt attggattgg gaccacacca gctaaacagg aaacgtggaa
     2161 atttcttggc acattagttt ctgcggcaac agttggcatt gtgatcatga ttttaaataa
     2221 aacttatggc tttactggtg aaaaagctct ggtagccccg caggctaatg caatggctgc
     2281 tgtcatcgaa ccgttgatgt caggtggagg agctccatgg cttttatatg cttttggagc
     2341 actcattgct cttgtcttga attttttgaa agtccctgca ctggcttttg cactcggaat
     2401 gtttattcca tttgaattga acattccatt gttaatcggt ggtgggatta actggctggt
     2461 ttctactaga acaaaagata aaaaacttaa tcaggttcgt caggaaaaag gtactttact
     2521 ggcttcagga tttatagctg gcggtgcttt gatgggtgtg gtaagtgcag ctttacgttt
     2581 tggtggattg gattttatca ataataaatg ggctgaaagt gctgcagcgg aatggttggc
     2641 attgataatg tatgctgtgt tgattggcta tttcatctgg gatgtcatgc gaatttcacc
     2701 aaaagataaa caaaaaaatt aataattttg ttattacgtt taaagtttaa aatttcattt
     2761 ttgaataata ataagaaatt aaaagtatat gtttgaatca ttaacagaaa gattagaacg
     2821 ttcgtttaaa attttaaaag gcgaaggaat aattactgaa atcaatgttg cagaaacgct
     2881 caaggatgta agaagagctt tactggatgc tgatgtcaat tataaaattg caaaaacatt
     2941 tacagatacc gttaaagaaa aagcacttgg ccaagatgtt ctaaaatcat tgaaacccag
     3001 tcagttaatg ataaaaattg ttcatgacga actggctgaa ttgatgggtg gcacaagtac
     3061 agatattaat ttaaaaggtt cgccagcaat tattttgatg gcaggtttac aaggttcagg
     3121 gaaaacaact tttgcagcta aattagccaa ttttttaaaa acaaaacgag gaaaaaatcc
     3181 tttattggta gcttgcgatg tgtatcgtcc tgctgctatt gaacagttga aagtgttagg
     3241 agaacaaata caagtgccgg tttatgctga attagaaaac aaaaatccta tttctattgc
     3301 agaatcagca gttaaatatg caaagcttca cggttacgat gtgctgatta tagatactgc
     3361 tggtcgtctg gcaattgacg aaatgatgat gactgaaatt tctgccatta agcaagctat
     3421 tcagccacaa gaaattttgt ttgtagttga ttctatgaca ggtcaggacg cagtgaacac
     3481 agcaaaggag ttcaatgatc gacttgattt tgatggcgtc gtgctgacta aacttgatgg
     3541 tgatacgcgt ggtggtgctg ctctttccat acgttctgtt gttaacaagc ctataaaatt
     3601 tgtaagtacc ggtgaaaaaa tggatgcact ggacgttttc catcctgaac gaatggctga
     3661 tagaatttta ggaatgggcg atattgtttc gttggttgaa agagctcagg aacaatataa
     3721 tgaagaagaa gcacgacgac tgcagaagaa aattgcaaaa aatcaatttg attttaacga
     3781 ttttttgtca caaattcatc aaatcaaaaa aatgggcaat ttcaaggatc tggcttccat
     3841 gattccagga gtggggaaag ctctcaaaga tgtagatatt gatgaaaacg ctttcaagtc
     3901 gattgaagct attattcaat ctatgacgcc cgaagaacgt gaaaatcctt ccttactgaa
     3961 tggaagtcgt aaacaaagaa tagctcgagg cagtggaaca accatcgtgg aagtaaatag
     4021 gcttatcaaa cagtttgacc aaattagcaa aataatgcga gtggcaacag taggttctaa
     4081 taaaatgaaa atgaagaaga agaaataaaa gaatgttaac gtttttttct attagaaatg
     4141 ttgtcggcaa aaaagattga gattttttta taatgggtaa aaattgatag gttgttaaaa
     4201 tttcttcaca gaatttttgt atttgtttat ctcttattct ttagatgttt cccataattt
     4261 tataaacatt cttttcccgc tttcagtgtg gttataattt tctctaaaaa tggcatatta
     4321 aagaaattcc ttaaaattga ctatcctcgc cgcaagaagc gagatattac gccaatttta
     4381 tggcttcgcc accggaattt ctaaaaaaat aaaaacaaaa gctaccccga cgcaagcatc
     4441 ggggaattct tacgattaaa ttacttgaat ttacgtacca atatttttta attacaagct
     4501 actgactacc aattcttaac tttttttctg cttcttccag cacttttacc acttccaaac
     4561 ccgatcgtcc atcggctatt tcgatttttt tatccagatg ttcgataaaa tatttcaatt
     4621 cttcggtgag tggttgtttt tgttcataag gaatgattct ggtaggattt tcaactttca
     4681 cgggaatacc cttttcaaag tgaatgcgtt tatcgtaaaa tttaatctcc ttatcaatgg
     4741 aagcatcatc aaacgaaatc attcccttgc tgccaatgac caccaaccgt tgctccttaa
     4801 acgggtgaag ccacgaaaca aaaatatggg catgaacatt attcgggtaa tccaattgag
     4861 tgatggttac atcatacaca ttttcctgaa gaaattttga accttttgaa acaatttttt
     4921 cagccggatg ccccacgaaa taatccaaaa ccgaaatatc gtgcggagca aatgaccaga
     4981 aaacgttttc ttccgtacga accgttccca aatttaatct gttggaatag aaataataaa
     5041 gatttcccaa ttctccactg tcaattattt ccttgatttt ccgaatagcc ggatggaaaa
     5101 gcaatacatg tcccgccatc aattgaacgc ctttcctttc agccagttca acaagttctc
     5161 tggaagtttc ggtagacaag gtcatgggtt tttcaaccaa aaccgatttt ccactttcaa
     5221 taatcttttt ggtcacttcg taatgcagtg gagcaggaac agcaacggta aatccgtcaa
     5281 aatccttttc caatgcctct tccaattctg taaatccttc gacgggataa attttcagca
     5341 attcgttcaa acgtttggca ttggcttcaa cgataccgcc caaattgccc aattgataaa
     5401 gcgttttgat gtggttttgt ccccatttgc caccgccaac aacacatatt ttcttgttca
     5461 tatttaaagt ttataaacgt ttttagctgg tggaatgaca tttctcaaat ctacaatcaa
     5521 atgagcatgt tgcataataa agtctgcttt ataaatagaa tgattggttg taatcactac
     5581 taagtctgca ttctccagat ttttagcaga atattccgtt gattgaaaag aataaccttc
     5641 gggagtggta atggccggaa cataaggatc gttataggaa acaattccac ctttatttgc
     5701 aataatatca atgatttcca gcgcaggaga ctctcgatca tcatcaatat ctcgtttata
     5761 tgctacgcct aaaaagaaaa ttttacttcc attaatagct tttttctgtc tattcaaagc
     5821 tgatgtaatt ttattataca tgtggtgtgg catacgcata ttgatatgac cagcagtatg
     5881 aatcatggtt aaatcaaaat caaacttttt agcgatatat tccagataaa aaggatcgag
     5941 cggtatgcaa tgtccgccaa tgccaggacc tggataaaaa gcttgaaagc caaaaggttt
     6001 tgttttagca gcttcaatca cttcccagat attaatatcc attttcccgg ctaacaatgc
     6061 caattcattg attaaactga tattaatcag ccgataagta ttttcaagaa ttttgaccat
     6121 ttcggctaca cgcggagaac taaccagatg cacattttga atagcttttt tataaagtgc
     6181 ttcgccaatt ttcaatccct cttcgttgat agctcccaat acttttggtg tatttttagt
     6241 tttgtaagtt tggtttcctg gatcaacccg ttcaggagaa taggccagcc agaaatcaac
     6301 tccatgtttt agtccagatt cgcgttcaat aatcggcaac ataaaatttt ctgttgtagt
     6361 agggaaagta gtgctttcca gactgataaa agtacccagt ttcatgtttt gcccgatttc
     6421 gatgcaagca ctttcaataa agctcatatc tggtttttga aaaatatcca gtggcgttgg
     6481 cacacaaata atcagtgcat cacattcctt gatgcgagaa aagtcgtcag tggcagtgaa
     6541 tttaccatcg gaaataatct cgcaaaattc ttcgtcagaa atatcggaaa tataattttc
     6601 tccgctattt atttttttta ctttgtcggg atttttatca aatccaataa cttttataga
     6661 agcttgtgca aaagtagttg ctaatggcaa tcctacatat cctaatccaa ttattccaat
     6721 tataatttga ttggattcaa ttttctgcaa aaaatcttca gaagtaatca tcttttattt
     6781 acgaattaaa tatctaaatt tttttgataa tggacttaaa taggttttaa aaacaagccc
     6841 aatttttatt aggtttctta tatagaaaaa aattaaacgg caaacataat tgattgaata
     6901 acacagtagt ttgtctaaaa taagcctgtt atttcaagac ccaaatttag ttaataattt
     6961 tcattttttt tcaaagctga aaaaatttgt tgattttgtt ttatcattat atcacagaaa
     7021 ttgaaaaatt gcttgttgat ttttgatctc ataatataat attttttgtg gtttcttgtt
     7081 gtcaaaattt cctcttaaac catccagata acaatttacc aaaaacttaa ttcgtctcca
     7141 tttgttgtta tcataaaaaa taatcccagt aaaagattta agaaaatccc ttattaaaat
     7201 caaataataa ttcattcttt tgtgagaata ttttttacct aaccaaatta aatttctttt
     7261 gctgaaataa gttagccaga gtttatcata aggtattcta taagatttta tacccaaaaa
     7321 tttttttaat gatcctgcat ctttacctgc ttctttatga acaatgatac tatcaggaat
     7381 gagaagaatt cttcctatct gatgtaatct aaggcaatat tcaaagtcat catgatgaat
     7441 aaaaaactct tttttcggat atcctgcttt ttgaacaatt tttctgttaa ttaatagccc
     7501 aacaaaggaa gccatttcta tttcgacaaa aggattttta tacaatgcag gctcgacaga
     7561 ctgttgaaaa gcataaaaat tttgattcaa atctatataa cttctatgct cgaaatttag
     7621 cgaaccatca agaaaagtga cactacccgc caatgctgat acattcttac agtcgaaaaa
     7681 tctgcttaat ttttctaaag catcctttgc tggttctgca tcatcatcca ttaaccataa
     7741 ccaatcataa tctttttcat aagctctttt gacgccttcg taaaatccac cagcacctcc
     7801 cgtattttca ttcattctca cataatatat cgtaatgatt ttactttcat ctgatttttt
     7861 tactttaaaa tctatttccc acggttcttt taaattttct ggtggcaatt cggttaagta
     7921 atggttttct tttaaaatgt agggagtgtc accagtagaa gaattatcaa tgatataaat
     7981 ggcatcaagt gaaaatgttt gcttcaataa ggcattgaga cattcaatta gtaaatttcc
     8041 ccgattgtaa gtaacaacaa ttgcacaaac agaattttta accttttttt cttcttttat
     8101 gtccttagtc attttacttt ttcttcaaaa atttccagtg attttttaat tatatcatcc
     8161 atatcataat actgataatc agccagtcgt ccaaccaata ttaaattttt aaatttcttt
     8221 gccaattcta aatagcgatg ataaagttct aaattttttt ctttcaatat tggataaaaa
     8281 ggtaaatttt taccccactg ataatcttgt ggatattctt tcagaataac tgttttacct
     8341 gtttcaacag gatgaatatg cttaaattcg gtgattcttg taaaatcata atcattggga
     8401 taatttacca cagcaacttc ctgaaaataa
//
LOCUS       DAOSAS010000078         8400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_5530_length_8400_cov_13.274056, whole genome shotgun sequence.
ACCESSION   DAOSAS010000078 DAOSAS010000000
VERSION     DAOSAS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5530_length_8400_cov_13.274056"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..899
                     /locus_tag="PLR72_04785"
     CDS             186..899
                     /locus_tag="PLR72_04785"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOK36553.1"
                     /translation="MTKLSVNVNKVATLRNARGGNIPNVCQVAIDCERFGADGITVHP
                     RPDERHIRYADVWELKSVLKTEFNIEGYPSEKLLNLVKSVKPHQVTLVPDAPEAITSN
                     AGWDTVKNFDFLKDIVAELQEEGIRVSIFVDTNLKNIEYASLTGTDRVELYTEPYARE
                     YSTNREKAIAPFIEAAQLAKKLGLGLNAGHDLNLQNLRYLHENIPWLDEVSIGHALIS
                     DALYFGLQETIKQYKNQLL"
     gene            973..1665
                     /locus_tag="PLR72_04790"
     CDS             973..1665
                     /locus_tag="PLR72_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOK36554.1"
                     /translation="MNLLVILQAASDVATTVAEQTLPKMNLWTMTKYGGPIMIILAAM
                     LAIAIYLFIERLITINEAAKEDSFFMERIKDYIHDGKIEAALKLCRETNFPTARMIEK
                     GITRLGRPMNDILVAIENVGNMEIAKLEKGLVIMASIAGGAPMLGFLGTVTGMVITLY
                     NMSMKAGGTILLGDLSVGMYQAMVTTIGGLIVGILAYFAYNYLVSKIDSVVTLLESRT
                     MEFLDLLNEPVK"
     gene            1748..2164
                     /locus_tag="PLR72_04795"
     CDS             1748..2164
                     /locus_tag="PLR72_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOK36555.1"
                     /translation="MALKRKNKVEAGFVMVSMTDIIFLLLLFFVIASTLSSPNDIKID
                     LPQSKAKTSTPVITKVFIDGKSNYFVAHGNEKPIPITADLLESYIVKLAAQDSMTHIA
                     LYADQNIPYRNVVRVLDIANQHKIKIVIATKPFEEK"
     gene            2166..2891
                     /locus_tag="PLR72_04800"
     CDS             2166..2891
                     /locus_tag="PLR72_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOK36556.1"
                     /translation="MRKSQIYGIIGSAISCGIILLLLFVIKLSAPSNMPQEEGIVVSF
                     GESEEDMGRSKEQGNHLTFKGAASQQISSDKNEKLLTQSESSVALSEQKNDKRQSENI
                     SEQEELRKKRENEAIEKANVIATIFGNGGSSEGSSGNDNILQGNPAGKGVSNGHGWSL
                     SGRDLVGGLVTPRYEKNVEGKITVNIRVDENGKVVGASIGSPTTISDADIRKAAIEAA
                     LNTRFTTGKGMVSGTITYNFKLK"
     gene            2909..3454
                     /locus_tag="PLR72_04805"
     CDS             2909..3454
                     /locus_tag="PLR72_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOK36557.1"
                     /translation="MKAIIFLAEGFEEIEAIAPIDILRRAGIEVVTVSVSDKNDVCGS
                     HGILIKADALFKEIDFSTADLLCLPGGMPGTKNLDNHQELKDLIVCHANGGKNLGAIC
                     AAPSILGKLGLLSGKEAICFPGFEDQLVGAKLSNKKVVRDGNIVTAKAAGVAVEFALK
                     LVEMLKGKSASDQVAASIFAN"
     gene            complement(3570..3842)
                     /locus_tag="PLR72_04810"
     CDS             complement(3570..3842)
                     /locus_tag="PLR72_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOK36558.1"
                     /translation="MNKAELVNAVAAKSGLSKVDAKKALDAVIKSISEALINGDKVSL
                     VGFGTFSVVERSARAGINPATKKPIKIPAKKVAKFKAGSELANAIK"
     gene            4187..>4380
                     /locus_tag="PLR72_04815"
     CDS             4187..>4380
                     /locus_tag="PLR72_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOK36559.1"
                     /translation="MSNKSSFLGSIPIVVKNLIFINIILWLLSIVSPSLFARWGLKVD
                     LMDILGMHYWASEKFNVAQL"
     assembly_gap    4381..4480
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4481..4815
                     /locus_tag="PLR72_04820"
     CDS             <4481..4815
                     /locus_tag="PLR72_04820"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOK36560.1"
                     /translation="LNRFFRINNLSGFDTASLIELKRLFLNLPVTIGASGSIFGLLLA
                     FGWLFPDARLMIIFFPIPIRARIFVLMYAVVELFLGVASFSGDNVAHFAHLGGMLFGI
                     ILILLWKK"
     gene            4870..5760
                     /locus_tag="PLR72_04825"
     CDS             4870..5760
                     /locus_tag="PLR72_04825"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOK36561.1"
                     /translation="MNVFKNIKSSFRRSTSLTKLLSINIGIFILYQLVLIVLKLFNLQ
                     GLFLAEYLALPSSLMQVATHFWTLVTYMFFHAGFLHIFFNMLCLYWFGKIFLMYFSEK
                     QLVGLYLMGGWMAAFFYIFSFNIFPYFKSVVNSSILLGASGSIMAIIVAAAVASPNLE
                     IRVLLLGNIKLKYIAIVAVLTSFFGIASNNAGGEIAHLGGALYGYLFVVSLHKGKDMT
                     KWLNNILDNIVNLFSWKPAKKRKKIYYTERKMTDEEFNIEKTRRMKEIDRILDKIKES
                     GYDSLTKEEKKILFDQSNKF"
     gene            5810..6901
                     /locus_tag="PLR72_04830"
     CDS             5810..6901
                     /locus_tag="PLR72_04830"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOK36562.1"
                     /translation="MIKSVVKWLLLAINIGVALCLFFALLAANIHPQYFILPSYFALL
                     FPVIFILNGAFFIFWLIFRSWYFLLSFCFLLLSIPQLKNNFSFRFKQPQKIEAEQPIT
                     LLSYNTRMMDLLKKYEPKEENKVLQYVLTSDADIVCLQEFMVSPNEKYLTEEEVLSLF
                     NKYPYHHIYYRVKQKSSWLGIATFSKFPIVNQQTIAYVSSANASIFTDINIHGKIIRI
                     INNHLESNQLTEKDKSLPYQLKNNLDTKLIHDITLVFSKKLGAAYKIRASQADSVAVV
                     VKNSPYPLLVCGDFNDVPGSYTYCKIKDHLNDSFLERGKGFGWTFIERYYRFRIDYIF
                     HDSFFQLVDFKIDKVRYSDHYPLLAKFSI"
     gene            6971..7348
                     /gene="gcvH"
                     /locus_tag="PLR72_04835"
     CDS             6971..7348
                     /gene="gcvH"
                     /locus_tag="PLR72_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOK36563.1"
                     /translation="MKVPENLKYTKDHEWIRLEDDIAYVGITDFAQKELGDIVFVEVE
                     TVGEQLSAGDVFGTVEAVKTVSEIFLPVDGEILAFNKELENAPELVNEDPYGKGWIVK
                     IRVTNPSQYDTLLTATDYKNLIS"
     gene            7376..7882
                     /gene="purE"
                     /locus_tag="PLR72_04840"
     CDS             7376..7882
                     /gene="purE"
                     /locus_tag="PLR72_04840"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOK36564.1"
                     /translation="MNPRVSIIMGSTSDLKVMEKAAKLLDEFEIFFEMNALSAHRTPE
                     EVEDFAVHAEGRGIEVIIAGAGMAAHLPGIIASMTTLPVIGVPIEASMNGIDALLSMV
                     QMPPGIPVATVGINASLNAAILAIEMLALKDENINVRLKSYKKNLKQKIVEANEQLKE
                     VKYRFKTN"
     gene            8068..8289
                     /locus_tag="PLR72_04845"
     CDS             8068..8289
                     /locus_tag="PLR72_04845"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOK36565.1"
                     /translation="MKMWKWFIIIGILLIIIGLLLYLTPSIFSWFGHLPGDIRIEKEN
                     MRIYIPLTSLIVLSVLITFIINLIRYFKS"
BASE COUNT         2752 a         1152 c         1504 g         2892 t
ORIGIN      
        1 aaagtagaaa tatttccata ttatcttgaa ttttttaaac tatttttgtg ttgaatttaa
       61 ttttaccaat aaactaaata gcttttaatt taccgaagtt agagagctaa aatgtattta
      121 tttggtatgt tgttaaaatt aggggcataa aaatttactc acaataattt tataattaag
      181 ttgatatgac aaaattaagt gtaaatgtta ataaagtagc aaccttacga aatgcaagag
      241 gaggaaacat accgaatgtg tgtcaggtgg ctattgattg tgaacggttt ggtgcagatg
      301 gtattacggt ccatccacgt cctgacgaac gacatattcg ttatgcagat gtttgggagt
      361 taaaatccgt tttaaaaacc gaatttaata tagaaggtta tccttcggaa aagcttttga
      421 atttagtaaa atcagttaaa cctcatcaag ttacattggt ccccgatgcg cctgaggcta
      481 ttacttcgaa tgcagggtgg gatactgtta aaaattttga ttttttaaaa gatattgtgg
      541 ctgaattaca agaagaaggt atccgtgttt ctatttttgt tgataccaac ttaaaaaata
      601 tagaatatgc ttcacttaca ggtactgatc gagtggaatt atacacggaa ccttatgcaa
      661 gagaatattc aacaaatcga gaaaaagcta ttgctccttt tattgaagct gctcaattag
      721 caaagaaatt aggcttgggg ttaaatgccg ggcatgattt gaaccttcaa aatcttcgtt
      781 atttacatga aaatatcccc tggctggacg aagtttctat tggtcatgct ttaatttcag
      841 atgctttata ttttggactg caagaaacaa taaagcaata taaaaatcag ttattgtaaa
      901 tgatacataa aggaataaaa aaggcacata ttttgtaaaa aaatcagaaa aaacgattaa
      961 aattgatttc aaatgaatct attagttatt ttgcaagccg catcagatgt ggcaacaact
     1021 gttgctgaac aaactctacc taaaatgaat ttatggacaa tgaccaaata tggaggtcca
     1081 atcatgatta ttctggcagc aatgctcgcc attgcaattt atttattcat tgaaaggtta
     1141 ataaccatta atgaagctgc taaggaagat tctttcttta tggaaaggat aaaagattat
     1201 attcatgatg gtaaaattga agctgctttg aagctctgcc gtgaaacaaa ttttccaact
     1261 gctcggatga ttgaaaaagg aatcactcgg ttaggacgac ctatgaatga tattttggtt
     1321 gctattgaaa atgtaggaaa tatggagatt gcaaaattag aaaaaggtct tgtcattatg
     1381 gcttctattg ctggtggtgc gccaatgttg ggctttttag gaacagttac aggaatggta
     1441 ataactttgt ataatatgtc tatgaaagct ggggggacta ttctcctggg cgatttgtcg
     1501 gtaggaatgt atcaggctat ggttactact attggtggac ttattgtagg aattttggct
     1561 tattttgcct ataattatct ggtttccaaa attgatagtg tggtaactct tctggaatcg
     1621 aggactatgg aatttctgga tcttctgaat gagcctgtaa aataaacttc tgggttctat
     1681 gttagcgtta aaaagattta ttttttgagt tttttatcat ttataaaaaa ttaattcatt
     1741 ttttcccatg gctttaaaaa gaaaaaataa ggttgaagca ggctttgtaa tggtatcaat
     1801 gacagatatt atttttctgt tactgctctt ttttgtcatt gcttctactt tatcttcacc
     1861 caacgatata aaaatagatt tacctcaaag taaagctaaa acttcaacac ccgtaattac
     1921 aaaagtattt attgatggga aatcaaatta ttttgttgct catgggaatg agaaacctat
     1981 accaattact gctgatttat tggaatctta tattgtgaag ttggcagctc aagattccat
     2041 gacccatatt gctttatatg cggatcaaaa tattccttat cgaaatgtgg taagagtgct
     2101 agatattgct aaccaacata aaatcaaaat agttattgcg acaaaacctt tcgaggaaaa
     2161 ataacgtgag gaagtctcaa atatatggca tcattggatc agcaattagc tgtgggataa
     2221 tacttttatt gctttttgtt ataaaattat ctgctccatc taacatgcca caagaagaag
     2281 gaattgtagt cagttttgga gaaagtgaag aagatatggg aagaagtaaa gaacaaggca
     2341 accatttgac atttaaaggg gctgcttccc aacaaatttc atcagataaa aatgaaaaat
     2401 tattgactca atcagaatct tcggtagcat tatcagaaca aaaaaatgat aaaagacaaa
     2461 gcgaaaatat ttctgagcag gaagaattga ggaaaaaaag agaaaatgaa gcaattgaga
     2521 aagccaatgt cattgcaact atttttggta acggaggctc ttcggaaggt agttcaggaa
     2581 acgataatat tttacagggc aatccagcgg gtaagggagt ttcaaatgga catggctggt
     2641 cactgagtgg aagggatttg gttggagggc ttgtaactcc ccgctatgaa aaaaacgtag
     2701 agggtaaaat tacggtaaat attagagtag atgaaaatgg gaaagtagta ggtgcttcca
     2761 ttggttctcc tactacaatt tctgatgctg atattagaaa agcagctatt gaagcagctt
     2821 taaatacccg ttttacaact ggtaaaggaa tggtaagcgg gactattact tataatttta
     2881 aattaaaata aaataacagt gaaaaattat gaaagcaatc atatttttag cagagggctt
     2941 tgaagaaatt gaagccattg ctccgatcga tattttgaga agagctggga ttgaggttgt
     3001 tacggtttct gtctcagata aaaatgacgt ttgtgggtct catggcatac taataaaagc
     3061 tgacgcctta tttaaagaaa ttgatttttc aacagcagat ttattatgtt tgcccggagg
     3121 catgccggga acaaagaatc ttgataatca tcaggagttg aaagatttaa ttgtttgtca
     3181 tgcaaatggt gggaaaaatt taggtgctat ttgtgcagct ccttctattt taggaaaatt
     3241 aggcttattg agtggaaaag aggctatttg ttttcctggt tttgaagatc aattggtagg
     3301 tgcaaagtta tcgaataaga aagttgtaag ggatggaaat atcgtcacag ccaaagctgc
     3361 aggagtagca gtggaatttg ctctcaaatt ggtggaaatg ttaaaaggaa aatcagcttc
     3421 agatcaagtt gctgcctcga tttttgctaa ctgagcgaat ataaagaatg ataaaaaaac
     3481 taattttgat aaaacttttt gcttttaaaa gagaatgaaa aatactaata tttttcattc
     3541 tcttttgtaa tgttgcctga tttaaaaaat catttaatag cattggcaag ttcagaacct
     3601 gctttaaatt ttgcaacttt ctttgcagga atcttaattg gctttttggt agcaggattg
     3661 ataccagccc gagctgatcg ttcaacaact gaaaaagttc caaaaccaac taaacttact
     3721 ttgtcaccat taatgagtgc ttcagaaata cttttaatta cggcgtcaag agctttttta
     3781 gcatcaactt tgctaagccc tgattttgct gcaacagcat taactaattc tgctttgttc
     3841 ataagataaa ttttttaaaa aattaaacaa gtaaaaaact ctttatattc tgcaaatgta
     3901 cattattgca agtaatatgc aaatatttta tataaaaaat taatcatttt tttatatttt
     3961 cttacaaaat atgttagaaa acatattttt gttttatttt tcattacttt tgtgctaaaa
     4021 atttattatt taactcgttt tttagaaagt tattattcct attaaaaggt ttttttgtta
     4081 acaccataaa aaatccttgc tactaataac ttgataattt tgtgaatatt ttttagtttt
     4141 tgaaataatt ttaaagtggc tgtaaatata gtaaaaaaat aaataaatga gcaataagtc
     4201 ttctttttta ggttcaattc ccatcgtagt aaaaaatcta atttttatca atataatctt
     4261 atggttgcta tcaatagtat ctcccagttt atttgctcgt tggggactaa aagttgattt
     4321 gatggatatt ttaggcatgc attattgggc ttctgaaaaa tttaatgtag ctcaacttat
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcttaaatcg tttttttaga
     4501 atcaataatt tatcaggttt cgataccgct tcattgattg aactaaaacg tctattttta
     4561 aatttaccgg tgacaattgg tgcttcgggc tcaatttttg gtctattgct ggcttttggt
     4621 tggctttttc cggatgcaag attgatgatt atatttttcc ccatccccat cagagcacga
     4681 atatttgttt taatgtatgc agtcgtagaa ttatttttag gagtggcaag cttttcaggg
     4741 gataatgtgg ctcattttgc tcatttgggt ggaatgcttt ttggaattat cctgattttg
     4801 ttgtggaaaa agtgagacaa aaaacatttt aattgagttg ttttgagtac tgattttctt
     4861 ttcaaattta tgaatgtttt taaaaacata aaatcttcat ttcggcgaag tacttcgctc
     4921 actaaattat tatctatcaa catagggatt tttatccttt atcaattggt actgattgtt
     4981 ctgaaacttt ttaatttgca aggattgttt ctggcagagt atctagcatt gccttccagc
     5041 ctaatgcaag tagcaaccca tttctggacg ttagtaactt acatgttttt tcatgcaggc
     5101 tttctccaca ttttttttaa tatgttgtgc ctttattggt ttgggaaaat atttctaatg
     5161 tatttttctg aaaaacaatt agttggttta tatcttatgg gtggttggat ggcagctttt
     5221 ttttatattt tttcctttaa tatctttcct tattttaaat cggttgtaaa tagcagtata
     5281 cttttaggag cttcggggtc tatcatggct attattgtag ctgcagcagt ggcttcgccg
     5341 aatttggaaa tcagagtttt gctgttagga aatatcaaat taaaatatat tgctattgtg
     5401 gctgttctaa ccagtttctt tggtattgct tccaataatg caggcggtga aattgctcat
     5461 ttaggaggtg ccctatatgg ttatcttttt gttgtatctt tgcataaagg aaaggatatg
     5521 acaaagtggc tcaataatat tcttgataat attgttaatt tattttcatg gaaacctgct
     5581 aaaaaaagga aaaaaattta ttatacagaa agaaaaatga cggatgagga atttaacatt
     5641 gaaaaaaccc gccgaatgaa agaaatagac agaatccttg ataaaataaa agaatcaggc
     5701 tacgacagct taacaaaaga agaaaagaaa atattattcg atcaaagtaa taaattttaa
     5761 tttttatcag tttttgtata attttgaatg attaaactga taagtatata tgattaaaag
     5821 tgttgtcaaa tggttgttgt tggctattaa tattggagtt gctctttgtt tgttttttgc
     5881 gttattagct gctaatattc atcctcaata ttttatactg ccatcttatt ttgctctgct
     5941 atttcctgtg atttttattc ttaacggagc cttttttatt ttttggctca tttttcggag
     6001 ctggtatttc ttactttctt tttgtttttt gttattatct attccccaat taaaaaataa
     6061 tttttccttt cgttttaaac agccacaaaa aattgaagca gagcaaccca tcactttact
     6121 ttcttacaat acaagaatga tggatttgct aaaaaaatat gaaccgaaag aagaaaataa
     6181 agtacttcaa tatgttctaa caagtgatgc agatattgtt tgtcttcaag aatttatggt
     6241 tagtcccaat gaaaaatacc ttaccgaaga agaggtattg agtttattca ataaatatcc
     6301 ttatcatcat atttattata gagtaaagca aaagtcaagc tggttaggaa ttgctacttt
     6361 ttcaaaattt cctattgtta atcaacaaac tattgcttat gtctcgtctg ccaacgcttc
     6421 tattttcact gacataaata ttcatggtaa aatcatcaga atcattaata atcatcttga
     6481 atccaatcaa ttaactgaaa aagataaatc gcttccttat caattgaaaa ataatctgga
     6541 tacgaaatta attcatgaca ttacgcttgt gttttcaaaa aaattaggtg cagcttataa
     6601 aataagagcc tcccaagctg attctgtggc agttgttgtt aaaaattcac catatcctct
     6661 tctggtgtgt ggcgatttca atgatgttcc gggctcatac acctattgta aaattaaaga
     6721 tcatttgaat gacagttttt tggaaagagg gaaaggattt ggatggactt ttatagaacg
     6781 ttattatcgg ttcagaatag attatatttt tcatgattct ttttttcaat tagttgattt
     6841 taaaattgat aaggtgagat attcagatca ttatccgtta ctagcaaaat tttctattta
     6901 actcaaaaat aagagctaat taatttactg aacactatac atttgtttaa cttttaatat
     6961 attattagaa atgaaagtac ctgaaaattt gaagtatacc aaagaccatg aatggataag
     7021 gcttgaagac gatatcgctt atgttggtat tacagatttt gctcaaaaag aattaggcga
     7081 tatagtattt gtagaagttg aaacagtagg tgagcaattg tcagcaggcg atgtttttgg
     7141 aacagtagaa gcagtaaaaa ccgtttcaga aatatttttg ccagtagatg gtgaaatttt
     7201 ggcttttaat aaagagttgg aaaatgcacc tgaattggta aacgaagatc cttacggtaa
     7261 gggatggata gtaaaaattc gtgttactaa tccttctcaa tatgatacgt tattaaccgc
     7321 tactgactac aagaatctaa ttagttgaat tttaataatt taaaaatcat taaaaatgaa
     7381 tccacgagta agtatcatta tgggtagtac ctccgatctg aaagtgatgg aaaaagctgc
     7441 taaattactg gatgaatttg aaatcttttt tgaaatgaat gccttatcag cccatcgtac
     7501 tccagaagaa gtagaagatt ttgccgttca tgcagagggg agaggaatag aagttatcat
     7561 tgctggtgca ggaatggcag ctcacttgcc aggtataatt gcttctatga ctactttgcc
     7621 tgtcattggc gttcccattg aggcttctat gaatgggata gacgctttgc tttcaatggt
     7681 acaaatgcca ccaggaattc ccgttgcaac agtaggtatc aatgcttcgt tgaatgctgc
     7741 catattagca attgaaatgc ttgcacttaa agatgaaaat ataaatgttc gtttaaaatc
     7801 atacaagaaa aatttgaagc aaaaaattgt tgaggctaat gagcagctta aagaagtgaa
     7861 atatcgtttc aaaaccaatt aatttttttc agactttatt tttgttggaa agaattattt
     7921 aattcgcaaa cttttggata tcaaccaaat ccttttgtaa aggatttgta atccgacaga
     7981 acagaataat tctgggataa attttatctc tatatttgct gattcaaaaa gattttttga
     8041 atttaaagaa gtaaaatttt taaaaaaatg aaaatgtgga aatggtttat aattataggt
     8101 atcttgttga taattattgg cttacttctt tatttgacac catcaatatt cagttggttt
     8161 ggccatttgc caggagatat acgcattgaa aaagaaaaca tgcgtatcta tattcctctc
     8221 acttccctga ttgttcttag tgtccttatt acttttataa tcaatttgat cagatatttt
     8281 aaatcataaa agacatcatt cgaggtagcc ttcagactat gtcgagctaa aaataaattt
     8341 tccagatttt aatcaaaaga attcctcgag gcttgtgtcc gggttaatcc caaagaaacc
//
LOCUS       DAOSAS010000079         8161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_5720_length_8161_cov_12.118061, whole genome shotgun sequence.
ACCESSION   DAOSAS010000079 DAOSAS010000000
VERSION     DAOSAS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8161
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5720_length_8161_cov_12.118061"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..907)
                     /gene="lgt"
                     /locus_tag="PLR72_04850"
     CDS             complement(35..907)
                     /gene="lgt"
                     /locus_tag="PLR72_04850"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOK36566.1"
                     /translation="MLNYVVWNVNPEIFRIGSLSVRWYGLMWAVGILLALFVGQRLFK
                     HEKLPEQWLDKLFFYAVIGGIVGARLGHCLFYEWELLPQPVKFLGITFKYGNYYLSHP
                     LEILYIWRGGLASHGGALGILVAMYFYNKNVSKKGFLWVFDRLVIGVALTGACIRIGN
                     LMNSEIYGTPTNLPWGFIFVRNGEVVPKHPTQIYEALYCLATFAVTWWLYWKKEAYKQ
                     TGLIFGVFLVMIFGIRFLLEFIKMDQEAFESGMILNMGQILSIPFIIWGIWLIVRRKK
                     TPAPALSTPKRKGS"
     gene            complement(931..4227)
                     /gene="secA"
                     /locus_tag="PLR72_04855"
     CDS             complement(931..4227)
                     /gene="secA"
                     /locus_tag="PLR72_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOK36567.1"
                     /translation="MALNNILKKILGNKSERDLNEIAPIIEKIQNVYPEIKALSNDDL
                     RARSQALKARINEYVAPEKNKIAELKASAETSEIDEQEKIYAEIDKLENEITDKYEKI
                     LNEILPEAFSIMKDTARRFVENEETEVTANDFDRNLAAKHDFVTIRGDKAYYKNEWMA
                     GGTLIKWDMIPYDVQLIGGVVLHQGKIAEMGTGEGKTLVATLPVFLNALTGNGVHIVT
                     VNDYLAKRDSEWMGPLYMFHGLSVDCIDKHAPNSEERRQAYLADITYGTNNEFGFDYL
                     RDNMAISPDDLVQRKHFYAIVDEVDSVLIDDARTPLIISGPVPKGDDQLFDELRPKVE
                     RLVNAQKNLVTKYLSDARMLLKSANEKEVEEGALALFRAYKGLPKNKPLIKFLSEPGI
                     KTTLLKTEEFYMQENNKNMPIATDPLYFVIDEKNNTIELTDKGIDLITDSSEDSDFFV
                     LPDVGSEIADLEKDTSLSLEEKQQRKDEILQNYAIKSERVHTIHQLLKAYTLFDKDVE
                     YVVMDNQVKIVDEQTGRILEGRRYSDGLHQAIEAKEHVTVEAATQTFATITLQNYFRM
                     YHKLAGMTGTAETEAAEFWDIYKLDVVVIPTNRPVIRDDMEDRIYKTKREKYAAVIEE
                     IVRLLDSGRPVLVGTTSVEISELLSRMLTGRKIKHNVLNAKLHQKEAEIVAEAGHRGT
                     VTIATNMAGRGTDIKLTPEVRDAGGLAIIGTERHESRRVDRQLRGRSGRQGDPGSSVF
                     YVSLEDDLMRLFGSERISKVMDRLGFQENEMIQHPMISRSIERAQKKVEENNFGIRKR
                     LLEYDDVMNSQREVIYTKRRHALMGERIESDIINMIYDEVDQIVETAKENANYGELQE
                     ELMTIFAIDVPFTEQEFNYSRTKDLVKIVAEAALNSFKRKMEKMAEIAYPVIKQVYEL
                     QGHKYENILIPISDGKKVYNITANLKTAYETQAKELVKSFEKQTLLYVIDNEWKEHLR
                     QLDELRNSVQNATYEQKDPLLVYKLESFGLFKEMIETINRKTLSILMRGQIPVREPEN
                     VKEAKIPAHQDYSKYKTQKEEFGNKGNGNKNDTREKQKPQPIHVEKKVGRNDPCPCGS
                     GKKYKYCHGA"
     gene            4831..6327
                     /locus_tag="PLR72_04860"
     CDS             4831..6327
                     /locus_tag="PLR72_04860"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOK36568.1"
                     /translation="MMDKLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALESLGVDIIE
                     AGFPVSSPGDFNSVVEISKTVTWPTICALTRAVEKDIEVAAEALKYAKRKRIHTGIGT
                     SDYHIKYKFNSTQEEILERSIAAVKYAKKFVEDVEFYCEDAGRTDNEYLARVVEAVIK
                     AGATVVNIPDTTGYCLPWEYGEKIKYLMENVKGIQNAVISTHCHNDLGMATANTIMGI
                     VNGARQAEVTINGIGERAGNTALEEVVMILKCHKSLNIETQINTKKIYPTSRLVSNLM
                     NMPIQPNKAIVGRNAFAHSSGIHQDGVLKNRENYEIMNPEDVGIDHNAIVLTARSGRA
                     ALKHRLSTLGVELDNDKLDMVYEEFLKLADKKKVINDDDILMLAGKERHEKNRIKLDY
                     LQVTSGVGIKPIASIGLIIGNEKFEAAATGNGPVDAAINALKKIINRKMTLQEFTIQA
                     INKGSDDVGKVHMQIEHEGKIYYGFGANTDIVAASVEAYIDAINKFVE"
     gene            6402..7799
                     /gene="leuC"
                     /locus_tag="PLR72_04865"
     CDS             6402..7799
                     /gene="leuC"
                     /locus_tag="PLR72_04865"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOK36569.1"
                     /translation="MKTLFDKIWDDHVVSTIENGPTQLYIDRHYVHEVTSPQAFNGLR
                     ARGLKVFRPEKTVLTADHNIPTLHQDKPVKDPISRNQLETFTRNATEFNLPYFFPMGH
                     PKNGIVHIIGPENGFTQPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEMVLATQCILQ
                     TRPKTMRITFKGKLNKGVTAKDLALFMILKLTTGGATGYFVEYAGEAIENMSMEERMT
                     VCNLSIEMGARGGMIAPDETTFAYVKDREFAPKGKEWEKKLAYWKTLKTDEGAHFDKE
                     YEFNAEEIQPMITYGTNPGMGISITESIPTIDEVNEASRVSFLKALNYMGFQPGEKLL
                     GKSIDYVFLGSCTNGRIEDFRAFANFVKGKKKADNVTAWLVPGSWKVEQQIREEGLLD
                     ILQEAGFEVRQPGCSACLAMNDDKIPAGKYAVSTSNRNFEGRQGPGARTILAGPLVAA
                     AAAITGKIADPREFM"
     gene            7834..>8161
                     /gene="leuD"
                     /locus_tag="PLR72_04870"
     CDS             7834..>8161
                     /gene="leuD"
                     /locus_tag="PLR72_04870"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOK36570.1"
                     /translation="MEKFIKLTSTVVPLPIENVDTDQIIPARYLKATDKEGFGNYLFA
                     DWRYNKDGSPKADFVLNDSTYKGSILVAGKNFGSGSSREHAAWAIAGYGFKVIVSSFF
                     ADIFRNN"
BASE COUNT         2618 a         1382 c         1425 g         2736 t
ORIGIN      
        1 aaaagtgagt gaaattttaa ttctccatta aattttaaga gccttttcgt ttgggagtag
       61 aaagagcggg tgcaggtgtt ttttttcttc ttacgattaa ccagattccc cagataatga
      121 atgggatact taaaatttgt cccatgttca aaatcattcc actttcaaat gcctcttgat
      181 ccatttttat aaattccaac aaaaatcgaa ttccaaaaat catgactaaa aacactccaa
      241 aaatcaaacc tgtttgttta taggcttctt ttttccagta gagccaccaa gtcacagcaa
      301 aagtagcaag gcaataaagg gcttcataaa tttgcgtagg atgttttggt accacttcgc
      361 catttcttac aaaaataaat ccccatggaa gattagtcgg agtaccataa atttcagaat
      421 tcattaaatt tccaatgcga atgcaagcac cggtaagagc aacaccaatc actaaacgat
      481 cgaaaaccca gaggaaacct ttttttgaaa catttttatt ataaaaatac atagcaacta
      541 aaattcccaa tgctcctccg tgacttgcca atccaccacg ccagatgtat aaaatttcga
      601 gcggatgaga taaataataa ttgccatatt taaaagttat gcctaaaaat tttactggtt
      661 gaggtaataa ttcccattca tagaaaagac aatgtcctaa acgagcgccg acaatgccgc
      721 caatcacagc ataaaaaaac agcttgtcaa gccattgttc aggtaatttt tcatgtttga
      781 aaagtcgttg cccaacaaag agcgctaaca atattcccac agcccacatt aagccatacc
      841 atcgtacgga caatgatcct attcgaaaaa tttcaggatt tacattccag acaacataat
      901 tcaatattgg attcattctt taattttcta tcaagctccg tgacaatatt tatatttttt
      961 cccgctgcca caaggacaag gatcatttct acccactttt ttttctacgt gaataggttg
     1021 cggtttttgt ttttcacggg tatcattttt attgccattt cctttgttcc caaattcctc
     1081 tttttgagtt ttgtatttac tgtaatcttg atgagcaggt attttagctt cttttacatt
     1141 ttctggttca cggacaggta tttgtcctct cattaaaatg gataaggttt ttctattaat
     1201 ggtttctatc atttccttaa aaaggccaaa ggattctaac ttatatacca ggagaggatc
     1261 tttttgctcg taggtagcat tttgaacaga attacgcaat tcgtctaatt gtcgtaaatg
     1321 ctctttccat tcgttgtcaa ttacgtataa taaagtttgt ttttcgaatg attttaccaa
     1381 ttcttttgcc tgagtttcat aagcagtttt taaattagct gtaatattgt aaaccttttt
     1441 accatcagaa ataggaataa gtatattttc atatttatga ccttgtaatt cgtaaacttg
     1501 tttaataaca ggataagcta tttcagccat tttttccatt tttcgcttga aactatttaa
     1561 agctgcttca gcaacaattt taacaagatc tttagtacga gaataattaa attcttgttc
     1621 agtaaaggga acatctatgg caaaaatagt cattaattct tcttgtaatt ctccataatt
     1681 agcattctct tttgccgttt ccactatttg gtcaacttca tcataaatca tgttgataat
     1741 atctgattca atgcgttcac ccattaacgc atgacgacgt ttggtataaa taacttcacg
     1801 ttgtgaattc atcacatcat catattcaag cagtcgttta cgtattccaa aattattttc
     1861 ttctactttc ttttgggctc gttcaatgga acgtgaaatc ataggatgtt gaatcatttc
     1921 attttcttga aatccaagcc gatccattac ttttgaaatt cgttctgaac cgaataaacg
     1981 cattaaatca tcttcaagcg aaacataaaa tacagaagaa cccggatcgc cttgacgacc
     2041 agaccgacct cgcaattgac ggtctacacg acgactttcg tgacgttcag taccgataat
     2101 ggcaagtcca ccagcatctc ttacttcagg cgttaatttt atatctgtac cacgacctgc
     2161 catattcgta gcgattgtaa cagtgcctct atgacctgct tccgctacaa tttctgcttc
     2221 tttttggtgc aatttggcat tcaatacgtt atgtttaatt tttctaccag taagcattct
     2281 gctgagtaat tctgaaattt caaccgaagt agttcccacc aatacgggtc gtccactatc
     2341 gagtaggcga acgatttctt ctataactgc cgcatatttt tcacgtttag ttttataaat
     2401 tctatcttcc atgtcatcac gaatcacagg acgattagta ggaataacca ctacatcaag
     2461 tttgtaaata tcccagaatt ctgctgcttc tgtttcagcg gttcctgtca ttccagcaag
     2521 tttatgatac attcggaaat aattttgcaa cgtaatggtt gcaaaagttt gcgtggcagc
     2581 ttcaaccgta acatgttctt ttgcttcaat tgcttgatgc aatccatcgg aatatcttct
     2641 tccttccaga atacggccag tttgttcatc tacaattttt acttgattat ccattaccac
     2701 atattcaaca tctttgtcga ataaagtgta ggctttcaat aattgatgaa tagtatgaac
     2761 tcgttctgat ttaatagcat aattttgcaa aatctcatct tttcgctgtt gtttttcttc
     2821 taatgataat gacgtatctt tttctaaatc agcaatttca ctacccacat caggtaaaac
     2881 aaagaaatcc gagtcttcac ttgagtctgt tatcaggtca atacctttat cggttaattc
     2941 aatggtatta tttttttcgt caatgacgaa ataaagagga tcggttgcta taggcatatt
     3001 cttgttattt tcctgcatat agaactcttc tgttttgagt aaagtagttt tgatacctgg
     3061 ttcactcaaa aattttatta aaggtttatt ttttggtaga cctttatatg ctctgaataa
     3121 tgcgagagct ccttcttcaa cttccttttc atttgctgat tttaacaaca ttcgtgcatc
     3181 cgataagtat ttggtgacca aattcttttg agcattaact aatcgttcta ctttaggacg
     3241 tagttcatca aacaattgat catcgccttt cggcactggt ccggaaataa ttaatggggt
     3301 tcgtgcatca tcaattagca cggaatccac ttcatcgaca atagcataaa aatgtttcct
     3361 ttgtaccaga tcatcgggac taatagccat attatcacga agataatcaa aaccaaattc
     3421 attgttggtt ccgtaagtga tgtctgccag ataagcttga cgtcgttctt cagaattagg
     3481 agcatgctta tcgatgcaat ctacgcttaa accgtgaaac atatataaag gacccatcca
     3541 ttctgagtca cgtttagcaa ggtagtcatt taccgtaaca atgtgaacgc catttcctgt
     3601 aagggcgttt aaaaaaacag ggagtgttgc aaccaatgtt ttaccttcac cagtacccat
     3661 ttcagcaatt ttaccttgat gtaaaaccac cccaccgatt aattgaacat cataaggaat
     3721 catatcccac ttgataagtg ttccacctgc catccattca tttttgtaat aagctttatc
     3781 gcctcgtata gttacaaaat catgtttagc tgccaaattt ctatcaaaat cattagcagt
     3841 tacttcagtc tcttcatttt ctacaaaacg tcgagcagta tctttcataa tgctgaaagc
     3901 ttctggaaga atttcattta aaatcttttc atatttgtcg gtgatttcat tttccagttt
     3961 gtcaatttcg gcgtagattt tttcttgttc gtcaatttca ctggtttctg ctgatgcttt
     4021 taattcagcg attttgtttt tttcaggagc tacatattca tttatcctag ctttcaaagc
     4081 ttgcgaacga gctcttaaat catcattgga aagagcttta atttcaggat aaacgttttg
     4141 gattttttct ataattgggg caatttcgtt taaatctcgt tcagacttat ttcctaaaat
     4201 tttttttaaa atattattta atgccattta tatcaatttt ttttagattc gattttatta
     4261 ttatgcaaac ctttataaaa caattttatt atcaaaaaga tttacaaaaa taacactttt
     4321 tatgtcgata gcctattatt tatatcaaaa attgttataa agctttcaaa aataaaaagt
     4381 tatcaacaca ttttcaaaaa taatttttct aagtattttg tttttctcat tttaactgtg
     4441 aataaaaaaa ccttttatac gaattatata aacaattgaa aaccaaattt ttaattgttt
     4501 gaaaagtatt aaagtagcat tttattatgg aaaaaattaa aaatcataat aaaatccatt
     4561 gaaaaaattt tattttatat ttgacttatt caaaaattga tttatctttg cagtgtaaat
     4621 aataatattg caatgaataa acattcctta catatacttc tactgggcct cattattcta
     4681 attttacaga ataagtgaag tgagtttggt attgtaggga tagtataaaa atgtctaaaa
     4741 gcctttctca ctcgcttagg agtgagaaag gcttttttta tgggaataag tggaaaaatt
     4801 ataggagttt ttatatttaa aaaattataa atcatggata aattatttat ttttgacact
     4861 actttacgtg atggcgagca agttcctggt tgtcaactga atacagttga aaaaattcag
     4921 gttgcaaaag ctttagaaag cttaggggtc gatataattg aagctggctt tcctgtttca
     4981 agtccaggtg attttaattc ggtagtggaa atttcaaaaa ctgttacctg gccaacaatt
     5041 tgtgctttaa cacgtgctgt tgaaaaagat attgaagtgg ctgcagaagc actaaaatac
     5101 gcaaaaagaa aacggattca tacaggtata ggaacttctg attatcatat caaatataaa
     5161 tttaattcta cacaggaaga aattttagaa cgttcaatag cagcggtgaa atatgcaaag
     5221 aaatttgttg aagatgtaga attttattgt gaagatgcag gaagaacaga caatgaatat
     5281 ctggcgcgtg tagtagaagc agtcattaaa gcaggtgcta cagtggtaaa tatacctgat
     5341 acaacaggtt attgtttgcc ttgggaatat ggagaaaaga ttaaatatct gatggaaaat
     5401 gtaaaaggca ttcagaatgc cgttatttct acccattgtc ataatgattt gggtatggca
     5461 actgccaata ctattatggg aattgtgaat ggtgctcgtc aggccgaagt aaccattaat
     5521 ggcattggcg aacgcgctgg aaatactgcc cttgaagaag tagttatgat tttaaaatgt
     5581 cataaaagct tgaacataga aacacaaatt aataccaaaa agatttatcc aaccagccga
     5641 ttggtttcta atttaatgaa tatgcctatt cagccaaata aagcaattgt tggcagaaat
     5701 gcttttgcac attcatcggg aattcatcag gatggagttt tgaaaaatcg agagaattat
     5761 gaaattatga atcctgaaga tgtagggatt gatcacaacg ccattgtttt gacagctcgt
     5821 agcggcaggg cagctcttaa acatcgtctt agtactttag gagtagagtt ggataatgat
     5881 aaacttgata tggtttatga agagtttctt aaattagctg ataagaagaa agtaattaac
     5941 gatgatgata ttttgatgct tgccggtaaa gaacgccatg aaaaaaatcg aatcaaactg
     6001 gattatttgc aagttacgtc gggtgtaggc ataaaaccca tagccagtat tggtcttatt
     6061 ataggaaatg aaaaatttga agctgcagct actggaaatg gacctgttga tgcagcaatt
     6121 aatgccctga aaaaaattat caataggaaa atgacgctcc aagaatttac tattcaagct
     6181 attaataaag gtagcgatga tgtagggaaa gtgcacatgc aaattgaaca tgaagggaaa
     6241 atttattatg gttttggtgc taatactgat attgtggctg cttctgtaga agcctacatt
     6301 gatgccatta ataaatttgt tgaatgatta ggaaattatt tcttattgat tgaattttag
     6361 tttggattaa aaaaacaaca aacaaatttt ttaaaagata tatgaaaact ttatttgata
     6421 aaatttggga tgatcatgtg gtttccacga tagaaaatgg tcctactcaa ttatacattg
     6481 acaggcatta tgttcatgaa gtaacaagtc ctcaggcttt taatggcctt cgtgcaagag
     6541 gtttaaaagt atttcgtcct gaaaaaacag ttttgacagc cgatcataat attcctacac
     6601 ttcaccagga taaacctgtc aaagatccta tttccagaaa tcaactggaa acttttacaa
     6661 gaaatgccac tgaatttaat ttaccatatt tttttccaat gggacatccc aaaaacggca
     6721 ttgtacatat tattggtcct gaaaatggtt ttactcaacc aggaatgacc atcgtttgtg
     6781 gagatagtca tacttcaact cacggagctt ttggggcaat tgcttttggt attggcacca
     6841 gtgaagtaga aatggtgttg gcaactcaat gtattttaca aactcgtccc aaaacgatgc
     6901 gaatcacttt caaaggtaaa ctgaataaag gcgtaacggc aaaagatctg gctcttttta
     6961 tgattttaaa attaacaacg ggaggtgcga caggttattt tgtagaatat gcgggagaag
     7021 ctatcgaaaa tatgagtatg gaagaacgga tgacggtttg caatttaagc atagaaatgg
     7081 gagctcgtgg tggtatgatt gctcctgacg aaaccacttt tgcttatgta aaagataggg
     7141 aatttgcccc caaaggaaaa gaatgggaga aaaaattagc ttactggaaa acattgaaaa
     7201 cagatgaagg agctcatttt gataaagaat atgaatttaa tgcagaagaa attcaaccta
     7261 tgattactta tggcaccaat cctggtatgg gaatatccat tactgaatct atccctacca
     7321 tagatgaagt aaacgaagca agtcgtgttt cttttctgaa agcattgaat tacatgggat
     7381 ttcaaccagg agaaaaatta ttggggaaat cgattgacta tgttttttta ggcagttgta
     7441 ctaatggaag aattgaagat tttcgtgctt ttgctaattt tgtaaaaggt aaaaagaaag
     7501 ctgataatgt cactgcatgg ttggttcctg gaagctggaa agtggagcaa caaatacgtg
     7561 aagaaggttt gcttgatatt ttacaagaag ctggttttga agttcgtcaa cctggttgct
     7621 cagcttgtct ggcaatgaat gatgataaaa ttcctgctgg aaagtatgca gtttcaactt
     7681 cgaatcgtaa ttttgaagga cgtcaaggac caggagcacg aactattctg gctggacctt
     7741 tggtagccgc tgctgctgcc ataactggta aaattgctga tccacgtgaa tttatgtagt
     7801 ttgttatttt taaatcatta aaaagaaatt aaaatggaaa aatttataaa attgacatca
     7861 acagtagtac cactgccaat agaaaatgtg gatactgatc aaataatacc agcccgttat
     7921 ttaaaagcaa ctgataaaga aggatttgga aattatttgt ttgccgactg gcgatataat
     7981 aaggatggtt cgccaaaagc agattttgtg ttgaatgatt ctacttataa aggttctatt
     8041 cttgtcgctg gtaaaaattt tggttcaggt tcaagccgtg aacatgcagc atgggctatc
     8101 gctggttatg gttttaaagt gattgtttcg agcttttttg ctgatatttt tcggaataat
     8161 g
//
LOCUS       DAOSAS010000080         8050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_5823_length_8050_cov_13.134459, whole genome shotgun sequence.
ACCESSION   DAOSAS010000080 DAOSAS010000000
VERSION     DAOSAS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5823_length_8050_cov_13.134459"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1115
                     /locus_tag="PLR72_04875"
     CDS             186..1115
                     /locus_tag="PLR72_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOK36571.1"
                     /translation="MWKDFFYFSKSQRIGIIVLIILILLVIIANLTLPLLPKKPVNVN
                     DSTFFAEVAAFKKSLVSQDSLRKVEWEQRYKERYSSFKKFSSYSKNSYQLFPFDPNTA
                     DSVTFVRLGLKPYIATNILKYRAKGGKFKHPDDLAKIYGISEEKFQELLPYIKIDENL
                     FISSNKIDENKKESEKIFVIELNSADTTQLMQVKGIGRTYAQRIVRYRKSLGGFVSVD
                     QLKEVYGMTPENYNRILPYCVVDPDLIQKIKVNTANVDRLNQHPYLNFYQAKAIYELR
                     RKKGKLYDFKELKSIPEFSDDELEKIQPYLSFE"
     gene            complement(1147..2340)
                     /locus_tag="PLR72_04880"
     CDS             complement(1147..2340)
                     /locus_tag="PLR72_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOK36572.1"
                     /translation="MSSTTTTKPGRLLSLDVLRGFTIAGMILVNNPGSWQYVYAPLRH
                     AQWHGLTPTDLVFPFFMFIMGVSMYLSYRKFDFKLTKQTFWKFTRRSVLLFLIGLILG
                     WFGLFCRELNQLNGVNMPLGEKILQAATQFENIRILGVLQRLALVSFFGTLIILLVRQ
                     KHIPWLIGFILLFYWILLGCTHSYEASVDNIIAIVDRAILGASHMYKETVADGSVIAF
                     DPEGILSTLPCIAHVLLGVWAGKLITETTNHEEKLQKLFIFGTILLFSGFLLDYGFPI
                     NKKIWSSSFVLVTCGFASLLLALLIWIIDIRQKKKWCVFFESFGVNPLFIYVVAGILS
                     ILLGNISFIYNGEWITIKNFVYTVWLAPVFGNYLGSLIYALCFVFINWIIGHILYKKR
                     IYIKL"
     gene            complement(2480..6100)
                     /locus_tag="PLR72_04885"
     CDS             complement(2480..6100)
                     /locus_tag="PLR72_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK36573.1"
                     /translation="MKLKKTNIVLLFILLLSLAGGMICEYLYRNPPKKAIQFNQFQNQ
                     LISKELKASRTLKMMKQLIENNSLDSLIKLPFPNNDISFYVFRDNELIFWSDHNLEVS
                     NIPLSDSTNLHFVQLPNAYCVSQSLNFDHYKLLSLIKIKNNFPYENQLLVNNFAKGFE
                     MDPRVGITIGSNLDQYAVFSTHGDYLFSLVPPSQPIYNENFAKLGLIFFLVFFLCFFI
                     FYARFPSLLHKKNIDIKTFVVAFLIIGLFVFVGIYFNFPETLYANHLFSPFQYAAHPF
                     LSSIVHLVIITGYFIASICLFYFYVKINRKVSRVTAFLFQLTLPLCFLLLYFLLKGIV
                     FHSGEQVNILNFKDFSPFTITIHILILGWGIGFGLLFYKIHRWIKKSHLLKIALVNEL
                     FFILCMGLITYFISRTDFKQVIISYILLCTPLYFFIFSKKRKNIYFQTGIYFFVFTVF
                     FIGNSFLFQQEKKLNKYKTLAQNIFINGTAENDPMTEILLQELDTQLINDTTFSFLLQ
                     NEDSLQSASKYISNTYLRGFWNRYEIHLNAATATSALYKEYQSFVEENGTLIKQTHFF
                     NIQPNEKNISHLGIFPVSSLKSDSLFLFIEFHPRQHVRSYSFPNLLISSTPDISTQYN
                     ISIARYENRKLVNSTGLVNFPNNSSWLPYKISEFYSFQQNHRTFYVYTPTRTTYLVIT
                     DNQPIVFSSYLFYFLYTFLSYYFIAWLMLQIYLYLKKGGKYSLGFTAKFQLSFIVLLL
                     LSFFGIFYVSVNFLQEKYRKEQIQNLETKKNYIQKALQDKYYWTQELNEKNVKILNFD
                     LQDLSYTYQIDIHVYNNDGILMASSQPLIFNKGLISNRIAPQPYFSENSNISQYESIG
                     KLNYLVAYTDFYNGDYLQIGYIAVPQFFSQDEIKNEIESFMSVIIQIYLVIIILVILL
                     TLLIGKQLSTPLRMLENKLKEMRIGKRNDKIDYKLNDEIGHLVAQYNLTVDQLEESAR
                     LLARSERESAWKSMARQIAHEINNPLTPMKLTIQQLKRTKQIDKERFDEYFAKSTEML
                     IEQIDNLSHIARTFSEFARMPDAKFEHLDVAQLLHSVVQLFSNNHSHIKISFLGKENG
                     IYVYADPNQMMEVFNNLLKNALEAIPENREGKIIAEITETDQKITIKISDNGIGIPDE
                     MKNKLFVPNFTTKNAGMGLGLAISKNIIELTGGSITYKTKLNEGTTFIISLPKEKNDL
                     FS"
     gene            complement(6111..6584)
                     /gene="rlmH"
                     /locus_tag="PLR72_04890"
     CDS             complement(6111..6584)
                     /gene="rlmH"
                     /locus_tag="PLR72_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOK36574.1"
                     /translation="MKFVLLTVGKTTDKHLLHLQEDYKNRIKFYLPFEIITLPEIKNT
                     KKLSVPEIVEKEGELISNYLQITDEVILLDEKGEHFTSVGFSEFISKKLLSSKRRIIF
                     VVGGPYGFAKEIYQRANRTIALSSMTFSHEMVRVIFLEQLYRAMTILKGEPYHHA"
     gene            6857..7291
                     /locus_tag="PLR72_04895"
     CDS             6857..7291
                     /locus_tag="PLR72_04895"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOK36575.1"
                     /translation="MKTLKLFFICSILFITNVLQAQTTAIPPDIISSFNNGDASRLSN
                     YLNDNVELVLGNNNNVYSKQQTLTILSDFFKKNKVNGFQVLHQGEKETSNFIIGTLKT
                     SNDDFRVYILMRKTNNKTLILQLRIEPSNESRLQGTDSFMAF"
     gene            7248..>8050
                     /gene="nadC"
                     /locus_tag="PLR72_04900"
     CDS             7248..>8050
                     /gene="nadC"
                     /locus_tag="PLR72_04900"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOK36576.1"
                     /translation="MNQDYKELIPLWLSEDIGDGDHTTLACIPADAKGKSRLIIKENG
                     VVAGIEVAKEIFHTFDPELSMTKFINDGDEVKIGDIAFEVEGKVQSLLQTERLMLNVM
                     QRMSGIATKTRQYVKALEGTKTKVLDTRKTTPGLRVLEKEAVRIGGGVNHRLGLYDMI
                     LLKDNHIDFSGGIKEAILRTKEYLKNKGKNLKIEIEVRNLQELSEVLTIGGVDWIMLD
                     NFDIENTQKAVEMVAGRYIIESSGGITLETLRSYAECGVDFISVGALTHS"
BASE COUNT         3017 a         1143 c         1236 g         2654 t
ORIGIN      
        1 catattttta tttgaaataa ttgaaaagaa atcattcaaa atcaatatga taaagtaaaa
       61 aatatttttt catttttact tgattttata ataacctgaa aaaaatttta tatcaacttt
      121 gtggtacgtt attaaaaaag taaaattata agtaaaacca ataattcttt taactgattc
      181 tgaggatgtg gaaagacttt ttttattttt ctaaaagcca acgaataggt attatcgtac
      241 tgatcatttt aattttactc gtcataattg ctaatttaac tcttccttta ttacctaaaa
      301 aacctgttaa cgtcaatgat tctacttttt ttgcagaagt agcagcattt aaaaaatctt
      361 tggtctcaca agatagtctc cgtaaagttg aatgggaaca acgctataaa gaacgttata
      421 gttcatttaa aaaatttagt tcctattcca aaaatagtta tcaactcttt ccatttgatc
      481 caaatactgc cgattctgtt acctttgttc gtcttggttt aaaaccttat attgccacta
      541 atattttaaa atatagagca aaaggtggaa aatttaaaca tcctgatgat ttagcaaaaa
      601 tctatggaat ttctgaagaa aagttccagg aactattgcc ttatattaaa attgatgaga
      661 atttatttat ttcttcaaat aaaatagacg aaaataaaaa agaatcagaa aaaatttttg
      721 tcatagaatt aaattcagca gatacaacac aattaatgca agtaaaaggt attggtcgaa
      781 cttatgctca gagaattgtt cgttatcgaa aaagtttagg cggttttgtt tccgtcgatc
      841 aattaaagga agtatatggt atgacgccag aaaattataa ccgaattctt ccttattgtg
      901 tcgttgatcc tgatttgatt caaaaaatta aagttaatac tgcaaatgtt gaccgcttga
      961 atcaacatcc ctatctcaat ttttatcaag ccaaagctat ttatgaactt cgacgaaaga
     1021 aaggcaaatt atacgatttt aaagagttga aatcaatacc tgaattttct gatgatgaat
     1081 tagaaaaaat ccaaccttat ttaagttttg aataattatt cagtaataaa aacaagttat
     1141 gtttttctat aatttaatat aaattctttt tttataaaga atatgaccaa taatccaatt
     1201 aatgaaaaca aaacataaag cataaatcaa ggaacccaaa taatttccaa aaacaggtgc
     1261 taaccaaaca gtatatacaa aatttttgat ggtaatccat tctccattat aaatgaagct
     1321 tatattccct aataatatac ttaatatccc tgcaactaca taaataaaca atggattaac
     1381 accaaacgat tcaaaaaata cacaccattt ctttttttgt cttatatcaa taatccaaat
     1441 aagtaaagca agtaacaatg aagcaaagcc gcaagtaacc aatacaaaac ttgatgacca
     1501 gattttttta ttaattggga aaccataatc aagtaaaaaa ccactaaaca gaagaatggt
     1561 tccaaaaata aataattttt gtaatttttc ttcatgattg gttgtttctg taataagttt
     1621 gcctgcccat acgcctaata atacatgtgc tatacaaggt aaagtgctta aaataccttc
     1681 aggatcaaag gcaattacac taccatcagc cacagtttct ttgtacatgt gagatgctcc
     1741 taatattgct ctatccacaa tagctattat attatcaaca ctagcctcgt aactatgagt
     1801 acagcctaac aggatccaat aaaacaataa aataaaacca ataagccaag gaatgtgttt
     1861 ttgacgaact aatagtataa tcaaggttcc aaaaaatgaa actaacgcaa gacgttgtaa
     1921 aacacctaaa attcgtatgt tctcaaactg agtagcagct tgtaaaattt tctcacctaa
     1981 cggcatgttg acgccattca gttgatttaa ttctcgacaa aaaagaccaa accatcccag
     2041 aattaaacca attaaaaaga gtaatactga acgacgtgta aatttccaaa aagtttgttt
     2101 ggtaagctta aaatcaaatt ttcgatacga taaatacata gaaactccca taataaacat
     2161 aaagaaggga aaaactaaat ctgttggagt taagccatgc cactgagcat gacgtaaagg
     2221 tgcataaaca tattgccaac ttccaggatt attaactaaa atcatacctg caattgtaaa
     2281 tcctcttaat acatctaacg aaagtaaacg tcctggtttt gtggtagttg tggatgacat
     2341 atttcagaaa ttttaaaaac aactttaact taatggacaa aaaataaaag gtaaaaattg
     2401 gagaaatgga attttcaatt ttttaaaaaa ctcaggtcac aaatttagaa gaaaaaaacg
     2461 aaacttgttt atagaaaaat tatgaaaata aatcattttt ttcttttggt aaagaaatga
     2521 taaaagtagt gccttcattt aacttcgttt tataagtaat acttccaccc gtcaattcta
     2581 taatattttt tgaaattgcc aaaccaagtc ccatgccagc atttttagta gtaaagttcg
     2641 gaacaaataa tttgtttttc atttcatcag ggatacctat tccattatct gatattttaa
     2701 ttgtgatttt ttgatccgtt tcagtaatct ctgcaataat ttttccttct ctgttttcag
     2761 ggattgcctc caaagcattt tttaataaat tattgaacac ttccatcatt tgattggggt
     2821 ctgcatatac atagatgcca ttttctttac ccagaaaaga aatttttata tgactatgat
     2881 tattactaaa aagctgaaca acagaatgta ataactgagc aacgtctaga tgttcaaatt
     2941 tagcatcggg cattcgagca aactctgaaa aagttcgagc aatatgagat aaattatcaa
     3001 tttgttcaat taacatttca gtggattttg caaaatattc atcaaatcgt tctttgtcaa
     3061 tttgtttggt tcgtttcagt tgctggatag tcaatttcat tggcgttaag ggattgttaa
     3121 tttcgtgagc aatttgcctt gccatcgatt tccatgctga ttctctttct gaacgtgcca
     3181 aaagtcgagc actttcttcc agttgatcta cagtgagatt atattgagct actaagtgac
     3241 caatttcatc atttagttta taatcaattt tatcattgcg tttaccaatt cgcatctctt
     3301 ttaatttgtt ctcaagcatg cgaagaggag tagaaagctg tttcccaatc aataaagtta
     3361 ataaaatgac cagtataata atgacgagat aaatttgaat aataacggac atgaaacttt
     3421 caatttcatt tttaatttca tcttggctga aaaattgtgg tacagcaata tagccaattt
     3481 gcaaataatc accattataa aagtcagtat aggcaacaag ataatttaat tttccaattg
     3541 attcgtattg acttatattg gaattttctg aaaaataagg ttgtggagca attcgattac
     3601 taatcagccc tttattaaaa attaagggtt gtgagcttgc cattaatatt ccatcattgt
     3661 tatatacgtg tatatcaatt tgataagtgt atgaaagatc ttgcaaatca aaattcaaaa
     3721 ttttaacatt cttttcattc aactcttgtg tccagtaata tttgtcctgc aaggcttttt
     3781 gaatataatt ttttttggtt tccagatttt gtatttgttc ttttcgatat ttttcctgga
     3841 gaaaatttac tgaaacataa aaaattccga aaaaactaag aagtagaaga acgataaagg
     3901 aaagttgaaa ttttgcagta aatccaagac tatatttccc tcctttttta agatataaat
     3961 aaatttgcaa catcagccag gcaataaaat aataggacaa aaaggtataa agaaaataaa
     4021 acagataaga agaaaataca ataggctgat tatcagtgat taccaaataa gtagtgcgcg
     4081 ttggcgtata gacataaaaa gttcgatgat tctgttgaaa actgtaaaat tctgaaattt
     4141 tgtaaggaag ccaggaagaa ttatttggaa aatttaccaa ccctgttgag ttgaccaatt
     4201 ttcgattttc atatcgagca atagaaatat tgtattgagt agaaatatca ggagtagaac
     4261 ttattaataa atttgggaaa ctataactgc gtacatgttg gcgtggatga aattctataa
     4321 aaagaaacaa agaatcactt tttagagaag atacagggaa aatacctaaa tgagaaatgt
     4381 ttttttcatt aggttgaata ttaaaaaaat gagtttgttt aataagtgtg ccattttctt
     4441 caacaaaaga ttgatattct ttgtataaag cagatgttgc agtcgctgca ttcaaatgta
     4501 tttcatatct gttccaaaaa ccacgtaaat aagtattgct aatgtatttg cttgctgatt
     4561 gaagcgaatc ttcattctgt aataaaaaag aaaaagtagt atcattaatt agttgggtat
     4621 ctaattcttg gagcaggatt tcagtcatcg ggtcattttc agcagtgcca ttaataaaaa
     4681 tattttgagc cagagtttta tatttattca gttttttttc ttgctgaaaa aggaaagaat
     4741 ttcctatgaa aaaaacagta aaaacgaaaa aatatattcc tgtttgaaaa taaatatttt
     4801 tccttttttt tgaaaaaata aagaaataca aaggtgtaca taataatata taggaaatta
     4861 ttacttgctt gaaatcagtt cgtgaaataa aataagtaat caaacccatg cagagaataa
     4921 aaaataattc atttacaagt gcaattttca ataagtgaga tttttttatc cagcggtgta
     4981 ttttgtaaaa taataatcca aaaccaattc cccaccccag tataagaata tgaatggtaa
     5041 tcgtaaaagg agaaaaatct ttgaaattta aaatattaac ttgttcacca gaatgaaaaa
     5101 caattccttt cagcaaaaaa tataacaaaa gaaagcataa tggtagtgtc agttgaaata
     5161 agaatgctgt aactcttgaa acttttctgt taatttttac atagaaataa aacaggcaaa
     5221 tactagcaat aaaatagcct gtaatgataa ctaaatgaac aatagaactt aaaaaaggat
     5281 gagctgcata ttgaaaaggg ctgaaaagat gattggcata taaggtttca ggaaaattga
     5341 aataaatacc tacaaaaaca aacaaaccaa taattagaaa agcaacaaca aaagttttaa
     5401 tatcgatatt ttttttatgt aaaagcgatg ggaaacgggc ataaaaaata aaaaaacata
     5461 agaaaaacac aaggaaaaaa attagtccta attttgcgaa attttcattg taaatgggtt
     5521 gtgaaggtgg aaccaaagaa aaaagataat caccatgcgt gctgaacact gcatattgat
     5581 ctaaattgga accaattgtg atacctactc ggggatccat ttcaaaacct tttgcgaaat
     5641 tgttaactaa caactgattt tcataaggaa aattattttt gattttaata agcgataaaa
     5701 gtttgtaatg gtcaaagttt aatgattgac taacacaata agcattgggt aattgaacaa
     5761 aatgtaaatt ggtagaatca gatagaggaa tattgcttac ttccagatta tgatctgacc
     5821 aaaaaatcag ttcattatca cgaaaaacat agaatgaaat atcattatta ggaaatggaa
     5881 gtttgatcag cgaatcaaga gaattgtttt caataagttg cttcatcatt tttaaggtgc
     5941 gtgaagcttt taattccttg ctaataagtt gattttgaaa ttgattaaat tgaatagctt
     6001 tttttggagg atttcgatat aaatactcgc aaatcattcc tcctgccaga gataatagca
     6061 aaataaacag taaaacaata tttgtttttt tcaacttcat ttcagcattt ttatgcatgg
     6121 tgatagggtt caccttttaa aatggtcatt gctctataaa gctgttcaag aaaaataact
     6181 cgcaccattt catgagaaaa ggtcatagaa gaaagagcaa tggtgcgatt agctctttgg
     6241 taaatttcct ttgcaaaacc atacggacca cctacaacaa aaattatgcg cctttttgaa
     6301 gacaaaagtt ttttagagat aaattctgaa aaaccaacag aagtaaaatg ttcacctttt
     6361 tcgtctaata atattacttc gtcagtgatt tgcaaataat tagaaattaa ctcaccttct
     6421 ttttcaacta tctcaggaac agataatttc ttggtatttt ttatttctgg caatgttatt
     6481 atttcaaacg gaagataaaa ttttatcctg tttttataat cttcctgcaa atgtaaaaga
     6541 tgcttgtcgg tagtttttcc aactgttaaa agtacaaatt tcatattgag ttagttgcaa
     6601 ttttttggct tgctatttga tatattattt tttagtacaa atatagaaaa actctttcag
     6661 gatgaaaaag ttagttacag ctgctttaaa aacaaaaaaa acattttttg ttgatttgaa
     6721 cgtgaaattt acaatttata tctattttga aaaatgaatc ataaattaaa attatctttg
     6781 taagattttg tagaggattt ctatttgaaa aataaaagaa aaacagaatc atataattta
     6841 ttttaaaatt ttagccatga aaaccttaaa attatttttt atttgcagta tattgtttat
     6901 taccaatgta ttgcaagctc aaacaactgc cattcctcct gatatcattt cgtcatttaa
     6961 caatggcgat gccagtcgat tgtccaatta tttaaacgac aatgttgaac tggtattggg
     7021 caacaataat aatgtttata gtaaacaaca aacattgact attctttctg atttttttaa
     7081 gaaaaacaaa gtgaatggtt ttcaagttct tcatcaaggt gaaaaagaaa cttctaattt
     7141 tatcattggt acactcaaaa cttcaaatga tgatttcaga gtatatattt tgatgagaaa
     7201 gacaaacaat aaaacattaa ttttacaatt aagaattgaa ccaagcaatg aatcaagatt
     7261 acaaggaact gattccttta tggctttctg aagatattgg cgatggtgac catactactt
     7321 tagcttgcat acctgctgat gcaaaaggaa aatcgagatt gattattaaa gaaaatggtg
     7381 ttgttgcagg tatagaagtt gcaaaggaaa ttttccatac attcgatcca gaactttcca
     7441 tgacaaaatt tattaatgat ggtgatgaag tgaagattgg cgatattgct tttgaagtcg
     7501 aagggaaagt gcaatctttg ctacaaactg aacgattgat gctcaatgtg atgcaaagaa
     7561 tgagtggcat tgcaaccaaa acgcgtcaat atgtaaaagc attagaagga acaaaaacga
     7621 aagtgcttga tacccgtaaa actactccag gtttacgtgt gttggaaaaa gaagcggtca
     7681 ggattggtgg tggtgtaaat catcgtttgg gtttgtatga tatgattcta ctgaaagaca
     7741 atcacataga tttttcaggt ggaataaagg aagccattct tcgaaccaaa gaatatttga
     7801 aaaataaagg gaaaaatttg aaaattgaaa ttgaggtacg taatttacaa gaactttcag
     7861 aagtgttaac tattggtggt gtggactgga ttatgcttga caattttgac attgaaaata
     7921 ctcaaaaagc tgttgaaatg gtagcgggtc gttatataat agaatcttcg ggtggcatta
     7981 cgcttgaaac acttcgttct tatgctgaat gtggcgttga ttttatttca gtcggagcac
     8041 tgactcattc
//
LOCUS       DAOSAS010000081         8048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_5824_length_8048_cov_11.336544, whole genome shotgun sequence.
ACCESSION   DAOSAS010000081 DAOSAS010000000
VERSION     DAOSAS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5824_length_8048_cov_11.336544"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLR72_04905"
     CDS             complement(<1..650)
                     /locus_tag="PLR72_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018920551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOK36577.1"
                     /translation="MTLNNLLQGIPLLEVIGDMNINISSIQFDSRKIQTGNVFVAIRG
                     TSVDGHQFIPSAIENGAVAIVCEEYPVKLNPNVTYVKVENSAEVLGKIACAWFGFPSS
                     KLILVGVTGTNGKTTVATLLYQTFRKLGYKAGLLSTVRNYIDEIPMEVTHTTPDPLEL
                     NEYLAKMVEAGCQYAFMEVSSHAIDQRRIAGLEFKGGIFTNLTRDHIDYHLTFENY"
     gene            complement(673..2808)
                     /locus_tag="PLR72_04910"
     CDS             complement(673..2808)
                     /locus_tag="PLR72_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOK36578.1"
                     /translation="MANNRKNIILRFALVYFLIVLAFLLVIYKIIVIQFVERDHWLAL
                     AAKNVKTNIVIKPNRGNIYACDGSLMASSIPTYYVYMDLRVPALHENDGALFKENIDS
                     VAICLSKFFGDKSAAQYKNDITKAYFEGNGEYRLYPKRISYAQLKILKTFPLFRLGKN
                     KSGLVTKELFERVKPFGSLASRTIGDIYADESKGGKNGIELAFDSVLRGTPGVGVRQK
                     IANSWQETVQVEPVDGKDVITTINIDFQDIAEKVLLDSLKAFDAEAGYVVLMEVQSGE
                     VKAIVNMQKNADGTYSENKNGVVSDLVEPGSTFKVVSLVAALDDGKVKITDTIDTGNG
                     IYQYANSFMKDHNYSRGGFHKITIAEAIQASSNIGISRMIVNAYGDNPAAYVNKLYQM
                     KLNVPMQLEIPGTAAPQIRHPLDKTRYWSKTTLPWMSIGYEVQIPPIYTLAFYNAIAN
                     NGKLIRPFFVKAISKNGQVVKTFQTETINHSICKPEVLKIVQETLLGVIEGKLGTAKN
                     VRSKYFRIAGKTGTAQISKGALGYKSGGKAHQVSFCGYFPAEHPQYTCIVVIREPHIG
                     YPSGGKMSGSVFKNIAERVMFLKSNAKPENLEVDTLSHKPKLPYCFSGRFCDLQTVAS
                     QMQLSVIGSPSDYVRVNTETQLRIEPINIMHNIMPDLINMGAKDAVYLAEKLGLKVQI
                     NGYGKLISQSISAGSPIQKGQSVLLNFQE"
     gene            complement(2811..3191)
                     /locus_tag="PLR72_04915"
     CDS             complement(2811..3191)
                     /locus_tag="PLR72_04915"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOK36579.1"
                     /translation="MSVFTKFFKSIIREEDWAEIKSSSFRDILNGNIFKKRFFTRQLG
                     LIAMIVVLLIIYIGNRYSYEKQLVYQNKLKKEIQDKKYESLTISAQLMQISRQSNVLK
                     MLNEKGIDLHELNEPLIILNDSVN"
     gene            complement(3226..4143)
                     /gene="rsmH"
                     /locus_tag="PLR72_04920"
     CDS             complement(3226..4143)
                     /gene="rsmH"
                     /locus_tag="PLR72_04920"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOK36580.1"
                     /translation="MNDGAYHIPVLLHEAVNGLNIQPDGIYVDATFGGGGHAREILNK
                     LNEKGRLIAFDQDEEATKNAFNDSRFTFVHSNFCYVKNFLRYLEIDKVNGILADLGVS
                     SHHFDASERGFSFRFEGKLDMRMNKRSPLTAADVLNTYSEEKLADIFFNYGELRNARK
                     IAKIIVENRDKQPLIQISSFVELIKPLFAKEKEKKDLARVFQALRIEVNHELEALQAF
                     LQQSLQVLQPQGRLVIITYHSLEDRMVKNFLKSGNFAGKIEKDFYGHPFLPFQLINNK
                     VIVPNNNEIEQNPRARSAKLRIAERKSLI"
     gene            complement(4154..4609)
                     /locus_tag="PLR72_04925"
     CDS             complement(4154..4609)
                     /locus_tag="PLR72_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOK36581.1"
                     /translation="MSTFIGKYEAKVDAKGRIFVPAAYRKLLGDIEKDRIVMRKEEEN
                     NCLIFYPEAVWNQKIEYLKSKLDEWNSIDELLLMQFVADAEWLVIDTQGRILVSKKNL
                     QTLGIENGEVVFMGMIDRFALWSKAHYEKARLSSDEFTSLLKEKLMKSE"
     assembly_gap    4998..5097
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5533..6216)
                     /locus_tag="PLR72_04930"
     CDS             complement(5533..6216)
                     /locus_tag="PLR72_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOK36582.1"
                     /translation="MLWAYIIFIVVAIVSFIIQNSLQNKFKKYSNLSIPNGMTGKDIA
                     EKMLHDHGIYDVKVTSTSGHLTDFYNPINKTVNLSEQVYNSSNVAAASIAAHECGHAV
                     QHAEAYAPLTLRSKLVPVVSFASSWMQWVLLAGIIFLNTFPQLMLAGIILFAITTLFS
                     FITLPVEINASQRALVWLNDAGITNYETHEPAKDALKTAAYTYVVAALGSLATLVYYI
                     LIFIGGSRR"
     gene            complement(6361..7632)
                     /locus_tag="PLR72_04935"
     CDS             complement(6361..7632)
                     /locus_tag="PLR72_04935"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOK36583.1"
                     /translation="MKIDVLLGLQWGDEGKGKIVDVLTPHYDIITRFQGGPNAGHTLE
                     FEGKKFILKSVPSGIFQGDKINIIGNGMVLDPALFKAEGEALETAGSPLTKLLKISKK
                     THLILPTHRLLDQAYENAKGVGKIGTTGKGIGPAYTDKVSRNGLRVGDIYHDFQNKYK
                     TAIGRHLDLLKFYQVDVDLSALEKEWFEGIEYLKRFELIDSEYYINKALSVGKKVLAE
                     GAQGTMLDIDFGTYPFVTSSNTICAGTCIGLGVAPSCIGEVYGIFKAYCTRVGSGPFP
                     TELKDETGEIMRKRGDEYGSVTRRPRRCGWIDLVALKYAIMLNGVSQLIMMKSDVLDN
                     FETIKACVAYKIDGKETTQFPFEVADNIEPVYVELAGWKTDLTKIKEEEKLPDNFKSY
                     LNFLEKELKIPIKIVSLGADREQTIVRNVCK"
     gene            complement(7710..>8048)
                     /locus_tag="PLR72_04940"
     CDS             complement(7710..>8048)
                     /locus_tag="PLR72_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOK36584.1"
                     /translation="LLYNAMQKDFRVSLATIYNTLDLLLECHLIVKHQFGDQLAQYEK
                     TFGTNIHHHLICTQCGKIKEFSDKNIRTAIQSKHFSNFQMTHYSLYVYGICSKCQNKK
                     KNRKNKSIKT"
BASE COUNT         2602 a         1486 c         1145 g         2715 t
ORIGIN      
        1 aaataatttt caaaagtcaa atgataatca atgtgatcac gtgtgagatt ggtaaaaata
       61 cctcctttaa attccagccc agcaatacgc cgctgatcaa tggcatgcga acttacttcc
      121 ataaaagcat attgacaacc tgcttctacc atttttgcca gatattcatt caattcaagt
      181 ggatcagggg ttgtgtgggt tacttccatt ggtatttcat caatataatt tcggacagtg
      241 gacaataacc ctgctttata tcccaattta cggaaagttt gatataaaag agtagcaaca
      301 gtagtttttc cattagttcc agtcacaccc accaatatca atttcgaaga aggaaagcca
      361 aaccaggcac aagctatttt tcccaaaact tcagcactgt tttcgacttt tacatatgtc
      421 acattcggat ttaattttac aggatattct tcgcaaacaa ttgccactgc cccattttca
      481 atggctgacg gaataaattg atgcccatca acacttgtac cacgtatagc aacaaaaaca
      541 ttccctgttt gaattttacg tgaatcaaac tgaatagacg aaatgttgat attcatatcg
      601 cctatcactt ctaaaagagg aattccctgt aaaaggttat ttaaagtcat aacatctaat
      661 ttattgaaat tattattctt gaaaattcaa taaaactgat tgcccttttt gaatcggcga
      721 gccggctgaa atgctttgag aaatcaattt tccatagccg ttgatttgta ctttcaagcc
      781 taatttttca gcaagataga cagcatcttt agctcccata ttgattaaat cgggcattat
      841 attatgcata atattaatag gctcaatcct taactgggtt tcagtattta ctcttacata
      901 atcgcttggt gaaccaatca ctgaaagctg catttgagaa gcaactgttt gtaagtcgca
      961 aaatcttcct gaaaaacaat aaggtaactt tggcttatgt gacaaagtat caacttccaa
     1021 attctcaggt tttgcatttg attttaaaaa catcacacgt tctgcaatat ttttaaaaac
     1081 tgaaccactc atttttcccc cagatggata gccaatgtga ggttcacgaa ttacaacgat
     1141 gcaggtatat tgtggatgtt cagcagggaa atagccacaa aatgatacct gatgtgcctt
     1201 tcctccagat ttatatccca atgctccttt tgatatttga gctgtaccag ttttgccagc
     1261 aatacgaaaa tattttgatc tcacattttt tgctgttcct aatttacctt ctattacacc
     1321 caataaagtt tcttgaacaa ttttaagtac ttcaggttta catatggagt gattaatagt
     1381 ttcggtttga aaggttttaa ctacctgtcc atttttactg atcgccttta caaaaaacgg
     1441 acggattaat tttccattat tagcaatagc attataaaaa gccaatgtat aaatcggcgg
     1501 aatttgaact tcgtagccaa tggacatcca cggtaaagta gtttttgacc aataccttgt
     1561 tttgtctagt ggatgcctga tttgtggtgc tgctgtacca gggatttcca actgcatcgg
     1621 cacattcaat ttcatctgat acaatttatt tacataagct gcaggattgt caccataagc
     1681 attgacaatc attctggaaa ttccaatatt ggatgaagct tggatggctt ctgcaatggt
     1741 tattttatga aatcctcctc gactataatt gtgatccttc ataaaagaat tggcatattg
     1801 gtaaatgcca ttaccagtat caatggtatc cgtaatttta acttttccat cgtccaaagc
     1861 tgctactaaa gatactacct taaaagtaga acctggttct actaaatctg atactacacc
     1921 atttttattt tcagaataag taccatcagc atttttttgc atatttacaa tggcttttac
     1981 ttctcctgat tgaacttcca ttaacactac ataaccagcc tctgcatcaa aagcttttaa
     2041 actatccaat aaaacttttt ctgcaatatc ttgaaaatca atgttaatag tggtaataac
     2101 atcctttcca tcaacaggtt ctacttgaac agtttcctgc caagaatttg caattttttg
     2161 tctcaccccg acacctggcg ttcctcttaa cacagaatca aaagccagct caattccatt
     2221 tttcccgcct tttgattcat cagcataaat atctcctatg gttcgcgaag ccagagaacc
     2281 aaatggtttc actcgctcaa acaattcttt tgtaactaaa ccacttttgt tttttcctaa
     2341 tctaaaaaga ggaaaagttt tcaatatttt caattgagca taagaaatgc gcttgggata
     2401 taaacgatat tcaccgtttc cttcaaaata agcttttgtt atatcattct tgtactgagc
     2461 agcagattta tcgccaaaaa atttagacaa gcagatagca acagaatcaa tattttcctt
     2521 aaaaagtgct ccgtcatttt catgtaaagc tggaactcgc aaatccatat aaacgtaata
     2581 agtaggaata gaacttgcca tcaaacttcc atcgcaagca taaatgtttc ctctgtttgg
     2641 ttttataaca atattggttt ttacattttt cgctgctaat gccagccaat gatcacgttc
     2701 aacaaactga attacaataa ttttatatat caccaataaa aaagcaagca ctattaaaaa
     2761 atataccaga gcaaaacgaa ggataatatt tttacgattg ttagccattt ttagttgact
     2821 gagtcattta atataatcaa tggttcattt agttcatgaa ggtctattcc tttttcgttc
     2881 aacattttca atacatttga ttgacggcta atttgcatta attgagcaga aatagttaat
     2941 gattcatatt ttttatcttg aatttctttt tttaatttat tttgatatac caactgtttt
     3001 tcataactat atcgattccc aatgtaaata atcagcagaa ctacaatcat agcaattaag
     3061 ccgagttgcc ttgtaaaaaa acgtttttta aaaatattgc cgtttaaaat atcccgaaaa
     3121 gaactggact taatttctgc ccaatcttct tcacgaataa tacttttaaa aaattttgta
     3181 aaaacagaca ttttggtgaa tttaaaaagt tgtaaaaaac catttttaaa tcaaagattt
     3241 tcgttcggct attcttaatt tggcactacg agctcttgga ttttgttcaa tttcattatt
     3301 attaggaaca attactttat tgttaatcaa ctgaaaaggt aaaaagggat gaccataaaa
     3361 atctttttca atttttcctg caaaattacc actttttaga aaatttttta ccattctgtc
     3421 ttccaatgaa tgataagtaa taatgaccag acgaccttgc ggttgaagca cttgtaatga
     3481 ttgttgcaaa aaagcttgta acgcttccaa ttcatggttt acttctatac gcaaagcctg
     3541 gaaaacacgc gccagatctt ttttttcttt ttctttagca aacaacggtt ttattaattc
     3601 cacaaaagaa gaaatttgta ttaaaggttg tttatcacga ttttcaacaa ttattttagc
     3661 aatttttctg gcatttctca attctccata attaaaaaaa atatcagcca atttttcttc
     3721 agaataggtg ttcaacacgt ctgcagcagt taacggagat cgtttgttca tacgcatatc
     3781 cagtttccct tcaaaacgaa aagaaaagcc ccgttctgaa gcatcaaaat gatgcgatga
     3841 tacacccaaa tctgctaaaa tgccatttac tttatctatt tcaagataac gtaaaaaatt
     3901 ttttacatag caaaaattac tatgaacaaa cgtaaaacgc gaatcattaa aagcattttt
     3961 ggtagcctct tcatcctgat caaaagcaat taatcgtcct ttttcattca gtttgtttaa
     4021 tatttcacga gcatgaccgc caccaccgaa agtagcatct acataaattc catcaggctg
     4081 aatattcaac ccattgactg cctcgtgcag caaaacagga atatgatacg ctccatcgtt
     4141 catcaggatt tttttattct gatttcatta atttttcttt caacaaactg gtaaattcgt
     4201 cagacgaaag tcgagctttt tcataatgag ctttactcca aagggcaaaa cggtcaatca
     4261 ttcccataaa aactacttcg ccattttcta ttcctaacgt ttgaagattt tttttcgaga
     4321 ctaaaatccg tccctgcgtg tctataacga gccattcagc atctgctaca aactgcatta
     4381 ataaaagctc atcgatagaa ttccattcgt ccagttttga tttcaagtat tcaatttttt
     4441 gattccaaac cgcttcggga taaaatatca aacaattgtt ttcttcttct ttacgcatta
     4501 caatgcggtc tttttcaata tctcctaaaa gttttcgata agccgcgggg acaaaaattc
     4561 ttcctttggc atctacttta gcttcatatt ttccgataaa tgttgacatt aattgagatt
     4621 tttactttac actgtaaaaa taaaaaaata aatttaaatt ccccacattt ccccacaaaa
     4681 aaattatcaa caattaatta acagcaaaaa atgcttttaa aaactttata aacagcttat
     4741 tgattataat tcgatcagaa aatatttgat ttaaaatact tattatcaaa atgttatgaa
     4801 ttttatttca taaaaatgga attaagtggg gaatattttt ttacttatta acaacttatt
     4861 aacagataac ttcatcccag aattgtcatt aggaatatat catttattta actgtagttt
     4921 ttaaagtatt gaaaatcgtt ttaaattttt tatacacaac aaacgatgat gtttttctat
     4981 tagagttgaa tttagcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntaa
     5101 gagtaagaaa aatattattt gctttttcca agcaagatac aagtaaatac ttacgtcagg
     5161 aatttatcaa aaaagttaat tggattggga gcaaaactcc taatacaaaa tgttattcaa
     5221 taaattttag gcaatttttg tcttgtagtt ttttataaaa ttaagcaaaa gctccttcca
     5281 ttcattttta aaaaaaataa gtctaatttt gataatgttt tttcggcaca acctattata
     5341 attcaaataa ttacaattta ttttctaata accgccatta gaaattaaat tctaatgata
     5401 aatttgggtt aaaaaaaatt gcattaacta cttgaattta cgttatgaaa gcaagttggt
     5461 attaaaaaaa ggaaaacctt ccataaaaaa cggcttagtt ttccttcttg taaaagttac
     5521 agttttattt ttttatctgc ggcttccgcc tataaaaatc aaaatatagt aaaccagcgt
     5581 tgccagcgat cccaaagctg ctactacata agtataagct gctgttttca aagcatcttt
     5641 ggcaggttca tgtgtttcgt agttagtaat tccagcatcg ttgagccata ccaaagcccg
     5701 ctgacttgcg ttaatttcta caggtaaagt aataaaacta aataaagtag tgatagcaaa
     5761 taaaataatt cctgctaaca ttaattgagg aaatgtgttg agaaatataa tccctgccag
     5821 caatacccat tgcatccacg aagaagcaaa actaactact ggaaccagtt tggatcgcaa
     5881 cgtcagggga gcataagcct cagcatgttg aacagcgtgc ccacattcat gagcagcaat
     5941 cgatgctgct gctacgttac tcgaattata aacttgttca cttaaattga ctgttttgtt
     6001 tataggatta tagaaatctg ttaaatgtcc cgacgtagaa gttactttta catcataaat
     6061 accatgatca tgtagcattt tttctgctat atcttttcct gtcatgccat tagggataga
     6121 taaattggaa tatttcttaa acttgttctg taaactattc tgaataataa agctaacaat
     6181 tgcaacgaca ataaaaataa tataagccca caacataatt tatttgttta aaagttcgac
     6241 agtaaataag caaaatcttt gccaaaataa aaatatgaca cagagatttt attgcatttt
     6301 aagtaaaata atttttccac cttcaaatcg caatcaatga atttttcatt gaataataat
     6361 ttatttacaa acatttctaa cgatagtttg ttcacggtca gcacccagtg atactatttt
     6421 tataggaatt tttaactctt tttctaaaaa attcagatag gatttaaaat tgtctggcaa
     6481 tttttcttct tccttgatct tagttaaatc agttttccaa ccagctaatt ccacataaac
     6541 aggttcgata ttgtcggcta cttcaaaagg aaattgagtg gtttcttttc catcgatttt
     6601 gtatgcaacg catgctttga tggtttcaaa attgtctaac acatcacttt tcatcatgat
     6661 aagttgagaa acaccattga gcataatagc atatttcaag gctactaaat caatccagcc
     6721 gcatcgcctg ggtcgtcgag ttactgaacc atattcatct ccccgttttc gcataatttc
     6781 tcctgtttca tctttcagct cagtagggaa aggaccgctg cccacgcgtg tacaataagc
     6841 tttgaaaata ccatagactt ctcctatgca gcttggagca actccaagtc caatacatgt
     6901 gccagcacaa attgtattgg aagaagttac aaatggataa gtgccaaaat caatatccag
     6961 catcgtacct tgagcaccct cagccaatac ttttttcccc accgaaagag ctttgttgat
     7021 ataatattca ctatcaatta gttcgaaacg tttcagatat tctattcctt caaaccattc
     7081 tttttcaaga gccgataagt caacatctac ctgataaaat ttaagcaagt caaggtgacg
     7141 accaatggct gttttgtatt tgttttggaa atcatggtaa atgtcaccca cacgcaaacc
     7201 gttacgactt actttatcag tataggcggg accgattcct ttacctgtgg taccaatttt
     7261 tccaacacct tttgcatttt cataagcctg atcgagcaaa cgatgggtgg gaagaatcaa
     7321 atgtgttttt ttagaaattt tcaataattt ggtcaacgga cttcctgctg tttccagtgc
     7381 ctcgccttct gctttgaaca aagctggatc cagtaccatt ccattgccaa taatattaat
     7441 tttatctccc tgaaaaatgc ctgaaggaac agatttcaaa ataaattttt tcccttcaaa
     7501 ttctaaagta tgacctgcat taggaccgcc ttgaaagcgt gtaataatat cataatgagg
     7561 agttaaaaca tctacaattt ttcccttgcc ttcgtcgccc cattgaagac ccaataatac
     7621 atctattttc atttttgaat tctttaattt tttctctgga attataaaat cactttcgtt
     7681 ataaaaatat tttctttttt gatgaaaatt taagttttga ttgatttgtt ttttctgttt
     7741 ttctttttat tctggcactt gctgcaaatt ccataaacat acaatgaata atgtgtcatc
     7801 tgaaaattgc taaaatgctt tgactggatg gctgttcgga tatttttatc agaaaattcc
     7861 ttgatttttc cgcattgtgt acaaataaga tgatgatgaa tattggtgcc aaaagttttt
     7921 tcgtattgtg ctaactgatc accaaactga tgtttgacaa tcaaatgaca ttccagcagt
     7981 aaatccaacg tattataaat ggtggcaagg ctaacccgaa aatccttttg catcgcatta
     8041 tacaataa
//
LOCUS       DAOSAS010000082         8009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_5856_length_8009_cov_12.439779, whole genome shotgun sequence.
ACCESSION   DAOSAS010000082 DAOSAS010000000
VERSION     DAOSAS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5856_length_8009_cov_12.439779"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLR72_04945"
     CDS             <1..518
                     /locus_tag="PLR72_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOK36585.1"
                     /translation="FYNGPKCGASAPDHMHFQAGTKDFLPLINDYHRLKSTHMELLEN
                     GDDYCLYQFKNYLRTVYCIESDKCKVVQQQFNKIYENLQKIAILQTEEPMMNIVCMFE
                     KEKWVVFIFPRQAFRPWQYSAEEDKRLLVSPATVEMSGIFITPVEEHFKKITKEDIVN
                     ILEQLSMNKIV"
     gene            559..1881
                     /locus_tag="PLR72_04950"
     CDS             559..1881
                     /locus_tag="PLR72_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOK36586.1"
                     /translation="MITTEPIIHVGILSSKKIEFIFHGFFVTAEGEKYSGKQKAEWKN
                     GKIFFDGKTSDELFFQPIDESNTFDLLDVVIGIHFHWERKENQRFKGSLRIIIEKDQL
                     TAINDIKAEDYLVSVISSEMSATSSLELLKAHAVISRSWLFYPLQNDKKNFFSQNSVE
                     NDTENRHIKWYERGEHEHFDVCADDHCQRYQGITRATSETVQLAIEVTRGEVLMFDHQ
                     ICDTRFSKCCGGVTETFENCWAPVKHPYLESIVDNSLPPQGFDLDLAIESNAQKWIRE
                     CPPAFCNTQDKKVLSQVLNNYDQETIDFYRWKVIYSQEEISEIIYRRSGFDFGKIIDL
                     IPLKRGKSGRIVELKIVGSKQTMVVGKELEIRKWLSTSHLYSSAFVVDKENFRQQIPG
                     RFILTGAGWGHGVGLCQIGAAMMSEKDYPYDRILLHYFRETELRKIYE"
     gene            2316..3263
                     /gene="cysK"
                     /locus_tag="PLR72_04955"
     CDS             2316..3263
                     /gene="cysK"
                     /locus_tag="PLR72_04955"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOK36587.1"
                     /translation="MSRIANNLTDLIGNTPLMRLSNYSKIKNLEAEIIVKIEAFNPCG
                     SVKDRVAKAMIEDAEKRGILKPCSEIIEPTSGNTGIGLAMVAAIKGYKLTLTMPETMS
                     IERRNLLKALGANIVLTPGALGMQGAIDKAIELHEKNSNSFIPQQFENPINPQIHRLT
                     TGEEIWQDTNGKIDIFVAGVGTGGTISGVGEALKTHNSNIKIVAVEPAGSPVLSGGKA
                     GTHLIQGIGAGFIPKNYNPAVVDEIIKVSDNDAILTSRLLAQQEGLLVGISSGAATYA
                     ATVLAQRRENKGKTIVALLPDTGERYLSTMLYAFEEYPL"
     gene            3288..>4574
                     /locus_tag="PLR72_04960"
     CDS             3288..>4574
                     /locus_tag="PLR72_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOK36588.1"
                     /translation="MENKKLKFETLQVHAGQEVDKTTKSRAVPIYQTSSYVFDDAAEG
                     ADLFALRKFGNMYTRLQNPTTDVFEKRMAALEGGIGALATASGQAAQFIALNNILQEG
                     DNLVSTSHLYGGTYNQFKNQFKRMGITAKFTENDEPKTFEKLIDEHTKALYVETMGNP
                     DLNVPDFDGIAAIADKYEIPLIVDNTFGAGGYLFRPIEHGAAIVVESATKWIGGHGTS
                     LGGVIVDSGKFNWGNGKFPSFTEPSESYHGLVFWNTFGPKSPYGNIAFIMRARVEGLR
                     DWGPAISPFNSFLLLLGLETLSLRMERHVQNALEIAQWLEKQPAVEFVNYPGLESSPH
                     HELAKKYLKKGFGGVLSFKLKGGAEAADKFINNLKLISHLANVGDAKSLILQPATTTH
                     EQLSPEDKIAAGVFPGLLRFSVGIENIEDIKEDIEQA"
     assembly_gap    4575..4674
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4676..5092
                     /locus_tag="PLR72_04965"
     CDS             4676..5092
                     /locus_tag="PLR72_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOK36589.1"
                     /translation="MITQNARLPFRDVAAECGVSRAAVHQRVQRLIDMKVITGSGYLV
                     DPKKLGYQMCVYIGITLERGSMYKKVIQELEKIPEIVESHYTLGPYTILIKLYAKNDE
                     HLMELLNGKIQEIKGVAATETLTSLDQRICRTIPIE"
     gene            5278..6258
                     /locus_tag="PLR72_04970"
     CDS             5278..6258
                     /locus_tag="PLR72_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOK36590.1"
                     /translation="MTILVTGANGLLGHWVVQELLDRNYTVKIVVRTWKNIFFDLKSV
                     EVITGNFTNYETLKKAATGCDAIVHLAATTATNLFHYEDYRKINVEGSAQVIRVANDL
                     QIKTVIYISSSNTIGYGSKDQPANEESPFQFPFTESFYARSKKEAEELFSKFASQKND
                     RHIIIIHPTFMIGAYDTKPSSGRLLLMGYKPRFMFVPKGGKNFVAARDVAVAICNALT
                     MGRNGEHYLAAGVNLSFKEFYTLQKQVGNYQQVIIKLPNFLLKIMGKIGDFFRWLGIK
                     TDVCTMNLQQLMIQEYYSNEKAKKELMMPQTDIKKAIEETLNWFKYRGWI"
     gene            6869..7966
                     /locus_tag="PLR72_04975"
     CDS             6869..7966
                     /locus_tag="PLR72_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HOK36591.1"
                     /translation="MKIVIDSNIPYIRNVFERVAEVAYLPYHQITNETIRDADALIIR
                     TRTKCNAQLLKGTHVKFIASATIGYDHIDADYCSAHGIKWTNAPGCNSLAVTQYIASA
                     LSFLVKKNYFNLSSQTIGVVGVGAVGSKVAKLAASFGMQVLLNDPPRARREGLEKFVS
                     LKEVCEKADIITFHPLLNMSGIDKTYHLADEDFFYALKRKPIIINAARGEVISTEALL
                     KAFEEGKISGMIIDCWENEPNINKELLMRCILGTPHIAGYSIEGKANAAAQSVKAVSR
                     FFGLGLDNWEPTDLPEPFPLKTTSFESPMDFFLQTYNIEADSQQLKSFPEGFENQRSN
                     YIFRREPKAYLNQLEEDFKEKLTKQFEIFFR"
BASE COUNT         2712 a         1215 c         1452 g         2530 t
ORIGIN      
        1 ttttttacaa tgggcctaaa tgtggcgctt ctgctcccga tcacatgcat tttcaagcag
       61 ggacaaaaga ttttttacct ctcattaatg attatcatcg attaaaatct acccacatgg
      121 aattattgga aaatggagat gattattgtt tatatcaatt taaaaattat ttgcgtactg
      181 tttattgcat tgaatcagac aaatgcaagg tagttcaaca acaatttaac aaaatatatg
      241 aaaatttgca gaaaatcgct atcttgcaaa cagaagagcc catgatgaat atagtttgca
      301 tgtttgaaaa agagaaatgg gttgttttca tttttccacg tcaagcattc aggccctggc
      361 aatattcagc agaagaagac aaacgtttac tggtaagtcc agccacagtg gagatgagcg
      421 gtatttttat tacacccgtt gaagaacatt ttaaaaaaat tacgaaagaa gacattgtta
      481 acattcttga acagcttagt atgaataaaa ttgtttgatt tttagtaact tattgttaaa
      541 tataaccatt taattattat gataacgact gaacccatca tccatgttgg aatattaagt
      601 tccaaaaaaa tagaatttat cttccatgga tttttcgtca ctgcggaggg ggaaaaatat
      661 tcagggaaac agaaagcaga gtggaaaaat gggaaaatat tctttgatgg aaaaacaagc
      721 gatgaactgt tttttcaacc catagacgaa tcgaatactt ttgatttatt ggatgtagtg
      781 attggcattc attttcattg ggaaagaaaa gaaaatcaac gatttaaggg tagtttaaga
      841 attattattg aaaaggatca attaacagca attaacgata ttaaagccga agattatctt
      901 gttagcgtga tttcaagcga aatgagtgca acttcttcgc ttgaactatt gaaagctcat
      961 gctgtaattt ccagaagttg gttgttttat cctttgcaaa atgataaaaa gaattttttt
     1021 tcacaaaatt ctgtagaaaa tgacacagaa aatcggcaca tcaaatggta cgaaagaggt
     1081 gaacatgagc atttcgacgt ttgtgccgat gatcattgtc aacgttatca aggaattacg
     1141 cgagctactt ctgaaactgt tcaacttgca attgaagtta ctcgcggcga agttttaatg
     1201 tttgaccatc aaatttgtga tacacgattt tctaaatgtt gtggtggtgt taccgaaact
     1261 tttgaaaatt gctgggcgcc tgtcaagcat ccttatttag aaagcattgt agataattct
     1321 ttgccgccgc aaggttttga tctggattta gcgatcgaat ccaatgcaca aaaatggatt
     1381 cgtgaatgtc cgccagcttt ttgtaataca caagacaaaa aagtcctttc tcaagttttg
     1441 aataattacg atcaagaaac aatagatttt taccgttgga aagtgattta cagtcaggaa
     1501 gagatttcag aaattattta tcgtcgttct ggatttgact ttggaaaaat tatagacttg
     1561 atacctttga aaagaggtaa gtcaggcaga attgtggaat taaaaattgt cggtagtaaa
     1621 caaactatgg tggtagggaa agagttagaa attcgcaaat ggctgtcgac aagtcactta
     1681 tacagttcag cttttgtagt tgacaaggaa aattttcgtc aacagattcc tggtcgtttt
     1741 attcttaccg gcgctggttg gggacatggt gtgggacttt gccagattgg agctgccatg
     1801 atgagcgaaa aagattatcc ttacgaccgt attttactgc attattttcg tgaaacagag
     1861 ttgaggaaaa tttatgaata actgtatccc gctaaaacaa agtagtttaa aaaaataaaa
     1921 tagttttttt aagataacta atagtcaaga gtttaaagtc ctgagtagag aaatagagga
     1981 tgtgcggttc gaaatatgag ttacaaaatc tggtatatga aaaatgagga ttttttgatc
     2041 ggaaaaattt cgatttataa caaggagaca attgaaacga aacaaaaatt ttcaaagtaa
     2101 ttaaaatcta aaatccccaa ataagaattt ttattcaaca gcctattttc aatacctttt
     2161 taaaagataa tttccaaaaa tttttcataa tttttctttc gttgataaag atttaagata
     2221 actttcgaat gattcaattc aaataaatgt tagttttaca aatagaactt ctaactttgt
     2281 gaattgataa ctttttaatc ttttaaaaaa ataagatgtc cagaattgca aataacttaa
     2341 cagatttaat tgggaatact cctttaatgc gactttcaaa ttacagtaaa ataaaaaatc
     2401 tcgaagcaga aataattgtt aaaattgaag catttaatcc ctgcggcagt gttaaagatc
     2461 gtgttgccaa agcgatgatt gaagatgccg aaaagcgagg aattttaaaa ccatgttcag
     2521 aaatcattga accaaccagc ggaaatactg gtattggtct ggcgatggta gcagccataa
     2581 aaggctataa attaactctt accatgcctg aaacgatgag cattgaacga agaaacctgt
     2641 taaaagcttt gggagccaat attgttttaa cacctggagc tttagggatg caaggtgcta
     2701 ttgacaaagc aatagaattg cacgaaaaaa attccaactc attcattcct caacaatttg
     2761 aaaatcctat caatccgcaa attcatcgat taaccactgg tgaagaaatt tggcaagata
     2821 caaatggcaa aatagatata tttgtagccg gagtaggaac aggaggaacc atcagtggtg
     2881 taggtgaagc tttaaaaact cacaattcta atataaaaat tgttgccgta gaacctgctg
     2941 gttcacctgt actttctggt ggcaaagctg gcacacatct tattcaagga attggagcag
     3001 gatttattcc taaaaattat aaccctgctg tggttgatga aattattaaa gtaagtgata
     3061 atgatgcaat tcttacttct cgattacttg ctcaacaaga aggattatta gtcggcattt
     3121 catctggtgc agctacttat gcggcaaccg ttctggcaca acgtcgtgaa aataaaggga
     3181 aaaccatcgt ggcacttttg cctgatacag gtgaacgcta tttatcaaca atgctctatg
     3241 cttttgaaga atatccgtta taaaattcaa taaatcagat aataattatg gaaaataaaa
     3301 aattaaaatt tgaaacgctt caagttcatg ccggacaaga agtggacaaa actaccaaat
     3361 caagagcagt tcctatttat caaactagtt cttatgtatt tgatgatgca gcagaaggag
     3421 ctgacttatt tgctcttcgt aaatttggga atatgtacac tcgtttacaa aatcctacta
     3481 ctgatgtttt cgaaaaacga atggctgctc ttgaaggtgg aatcggtgcg ttagctactg
     3541 cttcgggaca agctgctcaa tttatcgcat taaacaatat tttgcaagaa ggtgacaatc
     3601 tggtttctac ttctcattta tatggaggaa cttataatca atttaaaaat caattcaaga
     3661 gaatgggcat tactgcaaaa tttacagaaa atgatgaacc aaaaactttt gaaaagctaa
     3721 ttgatgagca tactaaggca ctttatgtgg aaactatggg aaatcctgat ttaaatgtac
     3781 ctgattttga tggtattgct gcaattgctg ataaatatga gattcctttg attgtcgata
     3841 atacttttgg agcaggaggt tatctgtttc gtcccattga acacggagct gcgattgtag
     3901 tagaaagtgc taccaaatgg attggcggac atggtacttc gctgggtggc gtaattgttg
     3961 atagtggaaa attcaattgg ggaaatggta aatttccttc ttttactgaa ccatctgaaa
     4021 gttatcatgg cttagtattt tggaatactt ttggaccaaa aagtccatat ggaaatattg
     4081 cttttattat gagagctcgt gtggaaggac ttcgcgattg gggaccagcc atcagtcctt
     4141 ttaattcttt ccttcttttg ctcggtttgg aaactctttc tttaagaatg gaacgacatg
     4201 tacaaaatgc actggaaatt gcacaatggc tggagaaaca acccgccgtt gaatttgtaa
     4261 attatccagg actagaaagc agtcctcatc atgaacttgc caaaaaatat ctgaaaaaag
     4321 gatttggcgg ggtcctttca ttcaaactaa aaggtggtgc tgaagctgct gataaattta
     4381 tcaataattt aaagctaatc agccatttag caaatgtagg tgatgccaaa tcattaattc
     4441 ttcaaccagc aacaactact catgaacaat tatcccctga agataaaatt gctgcaggtg
     4501 tttttcctgg cttacttcgt ttttctgttg gaattgaaaa tatcgaagat attaaggaag
     4561 acattgaaca agctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaattat
     4681 tacccaaaat gctcgtttgc catttaggga tgtagctgct gaatgtggag tttcccgggc
     4741 tgctgtacat cagcgtgttc aacgattgat cgatatgaag gtaattacag gttctggcta
     4801 tttagtggat cctaaaaaac tggggtatca aatgtgtgtt tatattggca ttacgcttga
     4861 acgtggctct atgtataaaa aagtcattca ggaattggaa aaaattccag aaatcgttga
     4921 atcgcattat actttgggac cttataccat tttgattaaa ttgtatgcaa aaaacgatga
     4981 acatctaatg gaattactga atggaaaaat tcaagaaata aaaggagttg ccgctactga
     5041 aacacttact tcgctcgacc aaagaatctg ccgaacaatt ccaattgaat aaaatttctt
     5101 aagtggaaaa tcaagaatcc cttaaaaact atttgacgat tttttaaatc cctttaaaat
     5161 atcatttaaa caaaatttaa attctattta ttgaatagtt ttctcctttc attctgataa
     5221 atttatcagg atgagtttta tttatttgtt gatcaatttt tttataaatt gatgtgtatg
     5281 acaattttag taacaggtgc aaatggatta ttgggacatt gggtcgttca agaattatta
     5341 gatcgcaatt ataccgttaa aatcgtagtt cgaacttgga aaaatatttt ttttgattta
     5401 aaatcagtag aagttattac tggcaatttt actaattatg aaacattgaa aaaggctgct
     5461 acaggatgtg atgccattgt tcatcttgct gctactacag ctacaaattt gtttcattat
     5521 gaagattatc gaaaaattaa tgtagaaggt tcagctcagg taattcgagt tgccaacgat
     5581 ttacaaatca aaacagtgat atacatcagt tcttccaata ccattggtta tggaagtaaa
     5641 gatcaacctg ccaatgaaga atctcctttt cagtttcctt ttactgaatc tttttacgct
     5701 cgcagcaaaa aggaagcaga agagcttttt tcaaagtttg cttcacaaaa aaatgatcgt
     5761 catataatta ttattcatcc cactttcatg attggagctt atgatacaaa acccagttca
     5821 gggagattgt tgttgatggg ttataaacca cgattcatgt ttgtcccaaa aggtggaaaa
     5881 aattttgtag ctgctcgcga cgtagctgta gctatttgca atgctttgac aatgggaaga
     5941 aatggcgaac attatctggc tgctggagtg aatctttcct ttaaagaatt ttacacattg
     6001 cagaaacaag taggtaatta tcagcaggta attattaaat tacccaattt tcttctcaaa
     6061 attatgggaa aaattggtga cttctttcga tggctgggca ttaaaacaga tgtttgcacc
     6121 atgaatcttc agcaattgat gattcaggaa tattatagca atgaaaaagc aaaaaaagaa
     6181 ttaatgatgc ctcaaaccga tataaaaaaa gctattgaag aaacgttgaa ctggtttaaa
     6241 tatcggggtt ggatttaatc caaatttttc atgaagaaga atttctaata gcagtcatta
     6301 gaaaaaatgt tttataatta tttaaattac aatacattta ttatattgac aaaattgaga
     6361 cggttttttg atgctgaaaa taagaaattc ttatccatta aaaaatatgc aatttttata
     6421 catacaataa atagccttat ttatagcatt ttacagaata ttactttcgt ttaggttagg
     6481 gtatttgttt ttaataataa ttctgaatta aggtagcaag cgtttttatt gttctcgcag
     6541 gaaaagggtt taaaattttg ttgtttacac gtaaacacat tacaacttcc ttacggaaaa
     6601 gattatgcca ggaatcttcc ttttggtaat agttggcagc agcatcccac ataagtttac
     6661 caggaaaaga aaattaatct ctttgttttt cattttttga ctttttaaac attttatctc
     6721 attttttcat taatatgttg aaatataata atatgtacaa atttttcaaa aaactttttc
     6781 aagcgttttt gtttttttat gacaataaat ataaataaac tacctttgaa agacttttca
     6841 gaggggcttc acttttaaaa ttgaaaaatt gaaaatagtt attgatagta acatacctta
     6901 tatacgaaat gtatttgaac gggtagctga agtagcttat ttaccctatc atcaaataac
     6961 caatgaaacc attcgtgatg ccgatgcgtt gattataaga acaagaacta aatgcaatgc
     7021 tcaacttttg aagggaaccc atgtaaaatt tattgcttct gctaccatag gctacgatca
     7081 cattgatgca gattattgtt ccgctcacgg aataaaatgg accaatgctc ctggatgcaa
     7141 ttcgctggca gtaactcaat atatcgcttc tgcactaagt tttttagtga aaaaaaatta
     7201 tttcaactta tcatcacaaa ctattggtgt agttggtgtt ggagcggttg gctcaaaagt
     7261 agccaagttg gcagcttctt ttggaatgca agtattatta aatgacccac cacgagccag
     7321 acgagaagga ctagaaaaat ttgtttcatt aaaagaagtg tgcgaaaaag ctgatattat
     7381 tacttttcat ccgctgctca atatgtcagg cattgataaa acttatcatc tggctgatga
     7441 agattttttt tatgccctca aacgaaaacc tattatcatc aatgctgcac gaggtgaagt
     7501 tatatcaaca gaagccttat tgaaagcgtt cgaagaagga aaaatatcag gaatgataat
     7561 tgattgctgg gaaaatgaac caaatattaa caaagaatta ttgatgcgat gcatattggg
     7621 aactcctcat attgcaggtt attccataga aggtaaagct aatgcggcgg cacaaagtgt
     7681 aaaagcagta agtagatttt ttggattggg cttggacaac tgggaaccaa ctgatttgcc
     7741 tgaacctttt ccacttaaaa ctacttcttt tgaatctcca atggattttt tcttacaaac
     7801 atataatatt gaagctgata gtcagcaact taaatcattt cctgaaggtt ttgaaaatca
     7861 acgttccaat tacatttttc gccgcgaacc aaaagcatac ctgaatcaac tggaagaaga
     7921 ttttaaagaa aaactcacca aacagtttga aatttttttc agataaaata atttcagaat
     7981 tttttctata tttgaattag taaagagct
//
LOCUS       DAOSAS010000083         7783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6057_length_7783_cov_13.676113, whole genome shotgun sequence.
ACCESSION   DAOSAS010000083 DAOSAS010000000
VERSION     DAOSAS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6057_length_7783_cov_13.676113"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLR72_04980"
     CDS             complement(<1..183)
                     /locus_tag="PLR72_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOK36592.1"
                     /translation="MRLPMFLKPMFFAGMDEVFYIAHPDCPRCCGCDPAVLFVQEVML
                     IRNHLAQNGKDLWIWGT"
     gene            complement(187..384)
                     /locus_tag="PLR72_04985"
     CDS             complement(187..384)
                     /locus_tag="PLR72_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36593.1"
                     /translation="MIPQINLLGHQSWESQIGKLLEVYPQFDETPSILLPTEYKWPMQ
                     MVYIAKVTAPSIQKFTKLFLM"
     gene            complement(477..683)
                     /locus_tag="PLR72_04990"
     CDS             complement(477..683)
                     /locus_tag="PLR72_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36594.1"
                     /translation="MKIVKGIFPAFVLLLFSFSAFCQSTFNVKGLCIGDPARQDVDDF
                     VKLIDKKLASAGGKYISFEDRLQT"
     gene            complement(755..>2569)
                     /locus_tag="PLR72_04995"
     CDS             complement(755..>2569)
                     /locus_tag="PLR72_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOK36595.1"
                     /translation="LGEEARYRKKDVLLGPGINIIRTPLCGRNFEYLSEDPFLISAMA
                     IPYIKALQSKDVAASVKHYLANNQEDHRTTVDVTMSERALREIYLPGFKAAVTEGGSY
                     TVMAAYNKFRGDWCSENEYLDREILRKELGFNGVLMSDWGGTHSTVKAALAGLDLEMG
                     TDIKDYNEWYFANPLIKAVQEGKVHMSVIDEKVANVLRIMMKTKVIDSKNREKGSMNT
                     PEHQKAAYNSAAEAAVLLQNNGNLLPLDFSKIKSIAVIGDNATRKHCGGGLSSEIKTL
                     YEITPLEALNHKFGENVTINFAQGYEKQSTFQEGSNIGQLNTDKVDWKLIDEAVAAAK
                     KSDVAIIFGGLNHDFDTESYDKPHMRLPYGQVTLIQEVAKANPNTVVVIIAGSPVELA
                     GIVNRVPSILWAWYGGMEAGNAIVDVLSGKVNPSGKMPFTLPVLLEQSPAHALGNYPG
                     RNLKVNYEEDILVGYRWFDTKNITPQFPFGYGLSYTTFNLDNLSTDKKVYGKDDIIYI
                     KFTVTNTGSRYGAEVAQIYVSDPVCSVLRPAKELKAFEKVFLQPGESKNIELPIKVSD
                     LAFYDEAKKEWVVEPGEFILQLGNSSRNISKTVTITFQ"
     assembly_gap    2570..2669
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3198..6008
                     /gene="uvrA"
                     /locus_tag="PLR72_05000"
     CDS             3198..6008
                     /gene="uvrA"
                     /locus_tag="PLR72_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOK36596.1"
                     /translation="MSKAGNIFVKGARVHNLKNIDAVIPRNKLVVITGLSGSGKSSFA
                     FDTLYAEGQRRYVESLSAYARQFLGRMSKPEVDFISGIPPAIAIEQKVNTRNSRSTVG
                     TSTEIYEYLKLLYARIGKTYSPISGTLVKKHEAEDVVETALSYPDGTTITIMIPIHLN
                     GDRNLKEQLQSFQIQGFSRVEIKGETWRIQDVLKNNLLIDTSEDIYLLVDRVTIDHTS
                     ATISRLTDSIETAFYEGDGICLLKVATEQKEKILSFSKKFEADGIEFEVPTEHMFSFN
                     NPLGACPVCEGFGKIIGIDEDLVVPNKSLSVYEDAIACWRGEVMSEWKKRLIATAHKF
                     DFPIHKPYNELTESQRQLLWTGNEYFPGINTFFKFLEENQYKIQYRVMLARYRGKTVC
                     PSCKGTRLKKEANYVKIGGKAITELILLSMEDLYQFFEKLQLDEYDQMIANRLLTEIK
                     NRIQFLLDVGLGYLNLNRLSNSLSGGESQRINLATSLGSSLVGSMYILDEPSIGLHPR
                     DTEKLIHVLKELRDLGNTVIVVEHDEEIIRAADYLIDLGPGAGRLGGEIVYTGEANVK
                     NDSSIENKIQNSWTLKYLIGTDEISIPAHRRKWNNYIEVIGASENNLKNLNVKFPLNV
                     MTVVTGVSGSGKSSLVEDVLYPALKKYYGGITDKPGKFSDLRGSLQLIHDVELVDQNP
                     LGRSSRSNPVTYIKAYDEIRKLFAEQQLSKQMGYTPSYFSFNTEGGRCEECQGEGVVT
                     VEMQFMADLVMECEQCHGKRFKQDILEVTYQGMNIYDVLEMTVNQAIEFFGQSKGTLE
                     KKIVKRLKPLQEVGIGYVKLGQSSSTLSGGESQRVKLASFLANEKDEPTLFIFDEPTT
                     GLHFHDIKTLLKAFDSLILKGHTILIIEHNMEVIKCADHIIDLGPEGGEKGGYLVFEG
                     TPEELIHCEASYTGKFLKEKLK"
     gene            6125..6224
                     /gene="ffs"
                     /locus_tag="PLR72_05005"
     ncRNA           6125..6224
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLR72_05005"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(6266..7591)
                     /locus_tag="PLR72_05010"
     CDS             complement(6266..7591)
                     /locus_tag="PLR72_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOK36597.1"
                     /translation="MSSLKDFFVRIYNFIRYDIWKVAETELSKGRKIVYRCVKTIIIA
                     TKGYIDDKLGIRASALTYSLFFAVVPIIALVISVGKGFGMEKMIENTLQKTFIAQADV
                     IPALMSFVKKYLETMQGGVFIGVGIVILLWSVTNFFRLIENTFNNIWQVKKSRSPIRQ
                     FTTYFSIILILPILIILSSGFSIFLNTTFSQISFLNFLSPVLNFGAKLLPYFLNWLIF
                     TLMYLLIPNTQVKFVNALIAGILAGTAFQIFQMLYIKGQINLSRYNAVYGGFAAIPLL
                     LLWLRISCLILLLGAEISYAAQSIHDYEFDLDIQNISLRYKKFISLFITYVIIKQFEQ
                     QKPPLSAQQIASEYKLPIRLVHQTLNQLVNMSILIEVFSEKTKSKTYQPALDIHQVTV
                     GMLYEKIETYGSDLFLSNKNEWLDAFWQKITVLQQKNKGNLSQVLVKDI"
BASE COUNT         2546 a         1331 c         1344 g         2462 t
ORIGIN      
        1 ggtcccccaa atccataaat ctttaccgtt ttgtgccaaa tgatttcgaa tgagcatgac
       61 ttcttgaaca aacaacaccg caggatcaca cccgcaacag cgtggacagt caggatgtgc
      121 aatataaaaa acttcgtcca taccggcaaa aaacatcggc ttcaaaaaca ttggtaatct
      181 catccactac atcaaaaata attttgtaaa cttctggatg ctgggggcag taacttttgc
      241 aatataaacc atctgcattg gccatttata ttctgttgga agcaaaatgg atggcgtttc
      301 atcaaattga ggataaactt cgagtaattt accaatttga ctttcccatg attgatgacc
      361 taacaagttg atttgcggaa tcaaacgaat atggttcttg tggcagatat ttacaatttt
      421 ttacttcctt ttctgaaaga gcatcctgag ctcgtaattc aggataagaa atatattcat
      481 gtctgtaatc tatcctcaaa actaatgtat ttacctccag cggaagctaa ctttttatct
      541 atgagtttga caaaatcatc cacatcttgc ctagcaggat caccaatgca taaacctttt
      601 acgttaaaag ttgattggca aaaggctgaa aatgaaaata aaagtaagac aaaagcaggg
      661 aatatccctt tcactatttt cataattcct tttcttaaat gaaagtaatt actttttata
      721 gaattaaaaa gataattatc aactgattaa ttttttattg aaaagtgata gtcacagttt
      781 ttgaaatatt tcttgaagaa tttcccagtt gcagaataaa ttctcccggc tctaccaccc
      841 attctttttt tgcttcatca taaaaagcca aatcggaaac ttttattggc agttcaatat
      901 tttttgattc tccgggctgt aaaaatacct tttcaaaagc tttcaactcc tttgctggac
      961 gcaataccga acaaaccgga tcgctcacat aaatttgagc cacttctgca ccataacgtg
     1021 agccagtatt agtaactgta aactttatat aaataatatc atctttccca tatactttct
     1081 tgtcggttga aagattatcc aaattaaagg tagtatagga caaaccatat ccaaatggga
     1141 attgtggtgt gatgtttttc gtgtcaaacc agcgatatcc tactaaaata tcttcttcat
     1201 aatttacttt taaattcctt ccgggataat ttcccaaagc atgagctggt gactgttcca
     1261 gcaaaacagg caaagtgaaa ggcatttttc ccgaaggatt tacttttcca ctcagcacgt
     1321 caactatagc attgcctgct tccattcctc cataccaagc ccacaaaatg gacggaacac
     1381 gattaacgat accggccaat tcaaccggag aaccggcaat gataacaaca accgtattgg
     1441 gatttgcctt tgcaacttcc tgaatgagtg ttacctgtcc atagggcaaa cgcatgtgtg
     1501 gtttatcata cgactcggta tcgaaatcat gattcaatcc tccaaaaata atggcaacat
     1561 ccgatttttt ggctgcagct actgcttcgt ctatcagttt ccaatccact ttatcggtgt
     1621 ttaactgtcc aatgttgctt ccctcttgaa aagtggactg tttttcataa ccttgagcaa
     1681 aattaatggt aacattttcc ccaaatttat gattcagagc ttccaaaggc gtaatttcat
     1741 agagagtttt tatttcggaa cttaatcctc cgccacaatg cttgcgggta gcattgtcgc
     1801 caattacagc aattgatttt atttttgaaa aatcgagcgg aagcaaattt ccattgtttt
     1861 gcagcaatac tgcagcttct gctgcggagt tgtaagcggc tttctgatgc tccggtgtat
     1921 tcatgctgcc tttttcacgg tttttactgt caatcacttt tgttttcatc attatgcgca
     1981 acacattggc tactttctca tcaatgaccg acatgtgaac ttttccttcc tgaacagctt
     2041 ttatcaatgg attggcaaaa taccattcgt tgtaatcctt tatatctgtt cccatttcta
     2101 aatcaagtcc tgccagagcg gcttttaccg tgctgtgtgt tccaccccag tcgctcatca
     2161 acactccatt aaagccaagt tcctttcgca aaatttcccg atcaagatat tcattttcgg
     2221 aacaccaatc accacgaaat ttgttgtaag ctgccatcac agtgtaagag ccaccttcgg
     2281 ttacggctgc tttgaagcct ggaagataaa tttctcgtag tgccctttca ctcatggtta
     2341 catcaaccgt agttcgatgg tcttcctgat tgtttgccag ataatgttta acacttgctg
     2401 ccacatcttt cgattgcagg gctttgatat aaggaatagc cattgcagaa atcaggaacg
     2461 gatcttcact caggtattca aaattccgac cgcaaagtgg cgtgcgaata atattgatcc
     2521 caggacccag caatacatct tttttacgat aacgtgcttc ttctcccaan nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tttagaaagt gttttaattt tttaattgtt
     2701 tcagtgattc tcgctcaatt atcttaacat aaaaataaac aaaaaattta acttcgttga
     2761 agcagctatg tagctttcaa aactgcatcg ttctatattt caaaactcga aacttgtcag
     2821 tttccggctc atcggataag gtcaattgaa aacaacatga ttaattgata aatataattt
     2881 gatataaaat ttatcagata tcaaaatgca aaacatttga gtaattttca atattttata
     2941 gacttttcat taacttcatt accattatat tataatgaca attctcagtt tagaaacaaa
     3001 aattctacaa ttgttgagtt aaatgtggat aatcatttta ttttattttc aagcactttt
     3061 ttaactgcga aacatttatt agtaaaatta ttttcagata aaaaactaat tttttatacc
     3121 attttattta taagagtagc ttattaattt ttgaggaatg ttgtatcttt gcagtttaaa
     3181 aaaatttctc taaaactatg tccaaagctg gaaatatttt tgtaaaagga gctcgtgtac
     3241 ataatttaaa aaatatagat gccgtcattc ctcggaacaa attggtagtc atcactggtt
     3301 tatctggttc aggaaaatct tcttttgctt ttgatacttt atatgccgaa ggacaaagac
     3361 gttacgtaga aagtctttct gcttatgcca gacaattttt gggaaggatg agcaaaccgg
     3421 aggtagattt tatttcaggt attccaccag ccattgccat tgaacaaaaa gtaaatacac
     3481 gcaattctcg ttccactgtt ggaacttcca cagaaattta tgaatacctc aaattacttt
     3541 acgctcgaat aggaaaaact tattctccca tttctggaac attagtaaaa aaacatgaag
     3601 ctgaagatgt tgtagaaact gctttgtctt atcctgatgg caccactatt accattatga
     3661 tacccattca tctaaatgga gaccgaaatt taaaagaaca attgcaaagc tttcagatac
     3721 aaggctttag tcgagtagaa ataaaagggg aaacttggcg aattcaggat gtgttaaaaa
     3781 acaatctttt aattgataca tctgaagata tttatttgct tgttgatcga gtaactattg
     3841 atcatacgtc tgctactatc agtagattga cagattctat tgaaacagcc ttttatgaag
     3901 gggatggtat ttgcctattg aaggttgcaa cagaacaaaa agaaaaaata ttgtcttttt
     3961 ctaaaaaatt tgaagcagat ggcattgagt ttgaagttcc aactgaacac atgttcagtt
     4021 tcaacaaccc acttggggct tgtcctgtgt gcgaaggatt cggaaaaatt atcggtatcg
     4081 acgaagattt agttgtaccc aataaatctc tatcagttta tgaagatgca attgcttgtt
     4141 ggcgaggtga agtaatgagc gaatggaaaa aaaggctgat agcgacggct cataaatttg
     4201 atttcccaat tcataaacct tataatgaac ttactgaaag tcaacggcaa ctgctctgga
     4261 caggcaatga atattttcca ggtataaata ctttcttcaa atttctggaa gaaaatcagt
     4321 acaaaattca atatcgtgtc atgctggctc gttatcgagg aaaaacggtt tgtccatcct
     4381 gcaaaggaac tcgactcaag aaagaagcaa actatgtaaa aattggcgga aaagcaatca
     4441 cagaacttat tttgctttct atggaagatt tgtatcaatt ttttgaaaag cttcaattgg
     4501 atgaatacga ccagatgatt gccaatcgcc ttttaactga aataaaaaat cgtattcagt
     4561 ttttgctgga tgtcggattg ggatatttaa acttgaatcg tttgtcaaat tcgctatcag
     4621 gaggagaaag tcagcgaatt aacctcgcaa cttcactggg aagcagtttg gtgggttcca
     4681 tgtacattct tgatgaacca agcattggct tacatcctcg tgatactgaa aaattaattc
     4741 atgttttgaa agaattaaga gatcttggca atacagttat tgtagtagaa catgatgaag
     4801 aaattattcg agcagccgat tatctgatag atttaggtcc aggagctggc agacttggtg
     4861 gtgaaattgt atatacaggc gaagctaatg taaaaaatga ttcttcaata gaaaacaaaa
     4921 ttcaaaattc ctggacttta aaatacttaa taggaacaga cgaaatttcc ataccagctc
     4981 atcgaaggaa atggaacaat tatattgaag taattggtgc aagtgaaaat aatttaaaaa
     5041 atctcaatgt taaatttcct cttaatgtaa tgacagtggt gactggcgta agtggttcag
     5101 gtaaatcatc gttagtagag gatgtcttat atccagcttt aaaaaaatat tatggtggaa
     5161 ttaccgacaa accgggaaaa ttttcagatt tacggggcag tttgcaatta attcatgatg
     5221 ttgaattggt ggatcaaaat ccattaggac gttcatctcg ctctaatccg gtgacttaca
     5281 ttaaagctta tgatgaaatc aggaaacttt ttgctgagca acaactttcc aaacaaatgg
     5341 gttacacccc ttcctatttt tcattcaata cagaaggcgg gcgttgcgaa gaatgtcaag
     5401 gcgaaggagt agtaactgtt gaaatgcaat ttatggctga tttggtaatg gaatgtgagc
     5461 aatgtcatgg aaaacgcttc aagcaagata ttctggaagt cacttatcaa ggaatgaata
     5521 tttatgacgt tttggaaatg actgtcaacc aggctattga attttttggg caatcaaaag
     5581 gaacgcttga gaaaaagatt gtgaaacgtt taaaaccttt gcaggaagtg ggaataggct
     5641 atgtaaaact tgggcagtcc tctagtacac tttcaggtgg cgaaagtcag cgggtgaaac
     5701 ttgcttcttt tctggcaaat gaaaaagatg aaccaacctt gtttatcttt gacgagccta
     5761 ctacaggtct tcatttccat gatattaaaa ctttactgaa agcttttgat tcgttgattt
     5821 tgaaagggca tactattctt attattgaac acaacatgga agtaattaaa tgcgctgatc
     5881 atatcataga tttaggtcct gaaggtggtg aaaaaggagg ttaccttgtt tttgaaggaa
     5941 cgccagaaga acttattcat tgtgaggctt cctatactgg gaaattttta aaagagaaat
     6001 tgaaataaac cttttatttt tgaatcgttt cctaaatttt aaaaagtgat aaatactttt
     6061 tagcgatttt gaaaattctt cctaaaaatt tggttagtat cttataaaag tatatttttg
     6121 tattgggtaa gtcctacacg accagctcct ttcaaactcc cccagggctt gaccgcagca
     6181 agggtagaaa gttgtagcgg tgcgatgtag ggggcttacc tttttttatg tatattttaa
     6241 ttttcagttt aatttcagga aaaaattata tatcctttac aagaacttga gacagatttc
     6301 ctttattttt ttgttgtaaa acggtaattt tttgccagaa agcatccaac cattcattct
     6361 tgtttgacag gaacaaatct gaaccataag tttcaatctt ttcatatagc attccaacgg
     6421 taacttgatg aatatctaaa gctggttgat aggttttaga ttttgttttt tctgaaaaaa
     6481 cttcaatcaa tattgacata tttactaatt gatttaaagt ttgatgtact aatcgaatgg
     6541 gaagtttata ttcagaagca atttgttgag ccgataaagg aggtttctgt tgttcaaatt
     6601 gttttattat cacataagtg ataaaaagag aaataaattt tttatagcgt aaacttatat
     6661 tttgaatatc cagatcaaat tcataatcat ggatactttg agctgcatag gaaatttcag
     6721 ccccaagcag aagaatcaaa caagaaattc gcagccagag taaaaggagt ggaatggctg
     6781 caaaacctcc ataaacggca ttataccgcg ataggttaat ttgtcctttg atatatagca
     6841 tctggaaaat ctgaaaggct gttcctgcta aaatacctgc tatcaatgca ttgacaaatt
     6901 ttacttgtgt attgggtatt aataaataca tcaatgtaaa aatcaaccag ttaagaaaat
     6961 aaggaagtaa tttagctcca aaatttagta cgggacttag aaaatttaaa aaagaaattt
     7021 gagaaaaagt ggtatttaaa aaaatagaaa aaccgctgga aagaataata agtattggaa
     7081 gaataagaat aatggaaaaa taagtggtaa attgtcgaat tggggaacgg gactttttta
     7141 cttgccagat attgttaaaa gtattttcta tcagtctaaa aaaatttgta acagaccata
     7201 ataaaattac aatgccaaca ccaataaaca cccccccttg catggtttcc agatattttt
     7261 ttacaaaaga catcagggcg ggaatcacat cggcttgagc tataaaagtt ttctgaaggg
     7321 tgttttcaat cattttttcc attccaaaac ccttaccaac agaaataacc agtgcaatta
     7381 taggaactac agcaaaaaaa agagaataag ttaatgccga agcccgaatg ccaagcttat
     7441 catctatata accctttgtg gctataatga tagtttttac acatcgataa acaattttac
     7501 gacctttact taattctgtt tcagcaactt tccaaatgtc gtaacgaata aaattataaa
     7561 tgcgaacaaa aaaatctttc aacgaagaca tcttatttaa ttttttagga gcttagaaat
     7621 aaaaaatatc ttcaaaaatt cgatgaaaat tttttgatta aatttaaaat ttatacaaca
     7681 tgtaatgaag ggattttttg aactttatca aaataaaaag gtaataataa aaaatagcag
     7741 gcttgtaagc cgggttctgt acttttttca aagcgtttgc cat
//
LOCUS       DAOSAS010000084         7685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6142_length_7685_cov_12.289777, whole genome shotgun sequence.
ACCESSION   DAOSAS010000084 DAOSAS010000000
VERSION     DAOSAS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6142_length_7685_cov_12.289777"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..942)
                     /gene="pnuC"
                     /locus_tag="PLR72_05015"
     CDS             complement(352..942)
                     /gene="pnuC"
                     /locus_tag="PLR72_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOK36598.1"
                     /translation="MDLVINYLAEHWVEIAGAVLSFIYLYLSINQKIGLWIFGFLCSA
                     LYVVVFFQSKFYAGMTLQLYYLGVSIYGWITWKRGEQATGNDLPVIHIPKSMILDVLA
                     VFLVIFIIYFFVLSNFTDSPLPRADSFTTAVSIIATWMLARKMIENWLLFIIADGICT
                     GLYFYRELYPTAILFAVYTIMAIIGYFEWKRTMNRE"
     gene            complement(964..2562)
                     /locus_tag="PLR72_05020"
     CDS             complement(964..2562)
                     /locus_tag="PLR72_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOK36599.1"
                     /translation="MKRHYLTIFILSILFIFNSFQSNGQGISLIQQRKLINALNIISN
                     MYVDSVNDKKLVETAIVSMLKELDPHSVYIPKEEVERINEPLEGSFEGIGIQFQMFQD
                     TLLVVQTIAGCPAEKVGIRPGDRIIYVDDKLIAGVKMQNSDIMKLLRGPRGTEVTVKV
                     LRREEPELLIFKIVRDKIPLYSVDANYLVRKDIGYIKINNFGNSTAAEFRKALTELQK
                     KGMKHLILSLQGNGGGYLGTAIQLADEFLEQGKLIVYTQGLRQPKKEWLATSHGDFEK
                     GKLIVLVDEYSASASEIVAGALQDWDRAIIIGRRTFGKGLVQQQIPLNDGSMLRLTTA
                     RYYTPTGRCIQKPYKDGIDKYEQELLNRHKKGELLHADSVHFPDSLRYQTLILHRTVY
                     GGGGIMPDIFVPLDTTRYTDYHQKLVARGVLNKVYIQYIDQHRNELTKNYSSFEKYKN
                     DFEVDDALLQQLIKEGEKEQIPYNEAQFNTSKELVKLQLKALIARDLWDVNAYYQIMD
                     VENSSLQKAIEILETPGAYEKILQ"
     gene            complement(2598..3263)
                     /locus_tag="PLR72_05025"
     CDS             complement(2598..3263)
                     /locus_tag="PLR72_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOK36600.1"
                     /translation="MTDSMEIFVDIHTHQLENQNYYSIINFDLQQAEKFLSTDTSFFC
                     SIGIHPWKVASITKEEVFQLKKWENDSRWIAVGECGLDKNSKASLTLQQYFFEKQITL
                     SEKLKKPLIIHCVKCFNEIIEIKKNLNPNQKWIIHGFRGKPQLAIQLLNNNIGISYGE
                     KFNPQSVEITPFEQLYVETDESKLPISEIYQRIALVKHCAVTKLNAGKKLLDLKENNN
                     LLI"
     gene            3383..4804
                     /gene="ahcY"
                     /locus_tag="PLR72_05030"
     CDS             3383..4804
                     /gene="ahcY"
                     /locus_tag="PLR72_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOK36601.1"
                     /translation="MSFSQLFSSLPYKVADITLANFGRKEIELAEKEMPGLMAIRQKY
                     ASQQPLKGARIAGSLHMTIQTAVLIETLVKLGAKVRWCSCNIYSTQDHAAAAIAAAGI
                     PVFAWKGETLEDYWWCTLQALTFEGGKGPNLIVDDGGDATLMIHTGMAAEKDANILEK
                     LPEGEDEKELLAILKKVLKEGRNFWSKMIPEIRGVSEETTTGVHRLYQMLEKGSLLFP
                     AFNVNDSVTKSKFDNLYGCRESLADGIKRATDIMLAGKVVVVCGYGDVGKGCAKSMRA
                     YGARVLVTEIDPICALQAAMEGFEVCTVEDALPEGNIYVTATGNRDVITIQHLEKMKD
                     GAIVCNIGHFDNEIQVDQLKKFPGIQKVNIKPQVDKYIFPDGHSILLLADGRLVNLGC
                     ATGHPSFVMSNSFTNQTLAQIELFTKKYKVNVYRLPKHLDEEVARLHLDQLGVKLTHL
                     TKEQAEYIGVNPEGPFKPEHYRY"
     gene            4996..5187
                     /gene="rpsU"
                     /locus_tag="PLR72_05035"
     CDS             4996..5187
                     /gene="rpsU"
                     /locus_tag="PLR72_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOK36602.1"
                     /translation="MIIIPVKEGENIDKALKKYKRKFEKTGVVRELRRRQSFEKPSIT
                     KRNQKLHAVYVQKLQSENQ"
     gene            5257..6138
                     /locus_tag="PLR72_05040"
     CDS             5257..6138
                     /locus_tag="PLR72_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOK36603.1"
                     /translation="MIKDFLDYLKYEKNYSSHTILSYKTDLLQFCDFLNTTPEALNVS
                     EVTSMQIQQWIASMMSEKKSKRTISRKISTLKSFWHFLLERKEVNQNPTLRIVLPKAK
                     KPLPSFFKNEEMEKALDETFLLENFEAQRDHLILEIFYLTGIRLSELISIQDSDIDLK
                     EGILRVIGKRNKERMIPLSKKLCEDIEHYIQLRDQQIEKKGNWLFVRKNGEKLYPKWV
                     YNKIHRTMSDVSTLNKKSPHVLRHSFATALLNEGADINAVKELLGHSSLAATQVYTHT
                     SFKELYNIYKQTHPRAK"
     gene            6152..6454
                     /gene="raiA"
                     /locus_tag="PLR72_05045"
     CDS             6152..6454
                     /gene="raiA"
                     /locus_tag="PLR72_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOK36604.1"
                     /translation="MKLRIQAINFEATSALESYVNKKLAKLEKLTDDIFNVEVYLKLL
                     KSSTGANKEVEIKVSIPSVEFFSSKVCETFEEATDLCIDAIEKQIKKHKEKFNYKK"
     gene            6565..6638
                     /locus_tag="PLR72_05050"
     tRNA            6565..6638
                     /locus_tag="PLR72_05050"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6599..6601,aa:Thr,seq:tgt)
     gene            6687..6769
                     /locus_tag="PLR72_05055"
     tRNA            6687..6769
                     /locus_tag="PLR72_05055"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6721..6723,aa:Tyr,seq:gta)
     gene            6821..6893
                     /locus_tag="PLR72_05060"
     tRNA            6821..6893
                     /locus_tag="PLR72_05060"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6854..6856,aa:Gly,seq:tcc)
     gene            6946..7017
                     /locus_tag="PLR72_05065"
     tRNA            6946..7017
                     /locus_tag="PLR72_05065"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6978..6980,aa:Thr,seq:ggt)
     gene            7078..>7685
                     /locus_tag="PLR72_05070"
     CDS             7078..>7685
                     /locus_tag="PLR72_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOK36605.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITKVLAEKGLSEV
                     RTFDSIDNAPEEKERGITINTSHVEYQTEKRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     IVVVAATDGPMPQTREHILLARQVNVPRLVVFINKCDLVDDEEMIDLVEMEMRELLNF
                     YDFDGDNTPVIRGSALGALDGDPKWEAKIEELMDAVDTWIQLP"
BASE COUNT         2623 a         1276 c         1315 g         2471 t
ORIGIN      
        1 atttattttt ataaaatgag tacaaaaggt tttagtgaaa caaaaaaatt aattcttctc
       61 aaataagaaa gattttgagc ttcatcaaaa aatttaaaaa attcccttaa tagaagtcaa
      121 taattaaata accggcggag ggttttttct tcgccggtaa aataaaaagt tcatattttt
      181 acgcattatt gtttatttta tttgtctata tggggaaatt ttaatattta tcttttttga
      241 aaattttttg tcaacacaaa acaaacttat agagggaaaa ttctttttaa gggattgtag
      301 ttaaaattat tcaatatatt aaagaatctc ttttttacga agttttgctt gctattctct
      361 attcattgtc cttttccatt caaagtagcc gattatagcc ataatggtgt aaactgcaaa
      421 taatatcgcc gttggatata attctctgta aaaatacaaa cccgtacaaa taccgtcagc
      481 aataataaaa agcaaccaat tttcaatcat tttgcgggca agcatccatg tggcaatgat
      541 gcttacagca gtggtaaatg aatcagctct tggaagaggt gagtcggtaa aattggacaa
      601 cacaaaaaaa tagataataa aaatgaccaa aaaaactgcc aatacatcca atatcattga
      661 ctttggaata tggataaccg gtaagtcatt gccagtagct tgttcccctc ttttccatgt
      721 aatccagcca taaatgctta ctccaagata atataactgc aaggtcattc ctgcataaaa
      781 tttactctga aaaaagacca ctacatataa agcagaacat aaaaaaccaa aaatccataa
      841 accaattttt tgattaatag aaagataaag ataaataaag cttaaaacag cccctgctat
      901 ttcaacccaa tgttctgcca aataattgat aactaaatcc ataagaattt ttagtttaga
      961 attttattgc agaatttttt cataagctcc tggagtttca agaatttcaa tggctttttg
     1021 taaagatgaa ttttccacat ccataatttg ataataagca ttgacatccc ataaatctcg
     1081 ggcaattaaa gctttcagtt gtaattttac caattctttt gaagtattaa attgtgcttc
     1141 attgtaagga atctgctctt tttcaccttc ctttatcaat tgttgtaaca aagcatcatc
     1201 tacttcaaaa tcatttttat atttttcaaa agagctgtaa ttttttgtca gttcatttct
     1261 atgttgatca atgtactgaa tatatacttt atttaaaacg ccgcgtgcta ctaatttttg
     1321 atgataatct gtataacggg tagtatcgag gggtacaaaa atgtcaggca taattccacc
     1381 acctccataa acagttcgat gaagaattaa cgtctgataa cgcaatgaat ctggaaaatg
     1441 aacactatct gcatgcaata gttctccttt tttgtggcgg ttcaataatt cctgttcata
     1501 tttatcaatt ccatctttat aaggtttttg aatgcaacgt cctgttggag tataataacg
     1561 agcagtagta agtcgtagca tcgaaccgtc gtttaaagga atttgttgtt gaaccagtcc
     1621 ttttccgaag gttcgtcttc caataatgat ggctctgtcc cagtcttgca aagctcctgc
     1681 tacaatttcg ctggcagaag cggaatattc atctaccagc acgattaatt tacctttttc
     1741 aaaatcaccg tgagaagtag caagccattc ttttttcggc tgtcgcaaac cttgtgtata
     1801 aacaattaat ttcccttgct ctaagaattc atcagccaat tggatggcag ttcccaaata
     1861 accgccacca tttccctgta aactcaaaat aagatgcttc attccttttt tctggagttc
     1921 agttaaagct ttacgaaatt ccgcagcagt agaatttcca aaattgttga ttttaatgta
     1981 accaatatct tttctcacca gataatttgc atccacacta taaagaggaa ttttatctcg
     2041 aacaatttta aatattaaca gttcaggttc ttctctcctt aaaaccttta ctgttacttc
     2101 ggtgccccgt ggtccccgca aaagtttcat tatatcggaa ttttgcattt ttaccccggc
     2161 aatcaattta tcatccacgt aaataatgcg atcacctgga cgaatgccaa ccttttcagc
     2221 cgggcagcca gcaatggttt gaacgaccag cagcgtatcc tgaaacattt gaaattgaat
     2281 accaattcct tcaaaacttc cttccagcgg ttcattaatg cgttccactt cttctttagg
     2341 gatataaact gaatgaggat ccaattcttt caacattgaa acaatagcag tttcaaccaa
     2401 ttttttatcg ttcacactgt ccacatacat attggaaata atattcaagg cattgatcaa
     2461 tttgcgttgc tgaatcaatg aaattccttg cccattactt tgaaaactat tgaaaatgaa
     2521 tagaatgctt aaaataaata ttgtcaaata atgtctcttc ataaaatttt gtatattgta
     2581 aaatttgtta tgtttttcta aatcaataaa ttattatttt cttttaaatc taataatttt
     2641 ttccctgcat ttaattttgt cactgcacaa tgttttacca aagctattcg ctgataaatt
     2701 tctgaaatgg gcaatttgct ttcatctgtt tctacataaa gttgctcaaa aggcgttatt
     2761 tctacacttt ggggattaaa tttttcgcca taagaaatac ctatattatt atttaacaat
     2821 tgaattgcca gttgaggttt tcctctgaat ccatgaatga tccatttttg attgggattg
     2881 agatttttct ttatttcgat aatttcatta aaacatttta cacaatgaat aatcagcggt
     2941 tttttcaatt tttctgataa tgttatttgt ttttcaaaga aatattgttg caatgtcaat
     3001 gaagcttttg aatttttatc cagtccacat tctccaacgg caatccaacg agaatcattt
     3061 tcccattttt tcagttgaaa aacttcttct tttgtgatag aagcaacctt ccagggatga
     3121 atgccaattg aacaaaaaaa ggatgtatct gtcgatagaa atttttctgc ctgttgtaaa
     3181 tcaaaattaa tgatcgaata ataattctga ttttctaact gatgcgtatg aatatcaaca
     3241 aaaatctcca ttgaatccgt cattatttaa aaggaaacat ttgacaaaag tagctatttt
     3301 tgaataaaaa cattaacttt gcacgcaatt atttagtaat aaagtttttc agaaagaatc
     3361 gaaccattca aaaaataaca aaatgagttt ttcacaatta ttcagttcat taccttataa
     3421 agtagctgat attactttag ctaattttgg tcgaaaagaa attgaattgg cagaaaaaga
     3481 aatgccagga ttaatggcaa ttcgacaaaa atatgcatct caacaaccat tgaaaggtgc
     3541 tcgtattgct ggttctttac acatgaccat tcaaacagca gttttaatag aaactctggt
     3601 aaaacttgga gctaaagtac gttggtgtag ctgtaatatt tattctacgc aggatcacgc
     3661 ggcagcagcc attgcagcag caggaatacc tgtttttgcg tggaaaggcg aaacgctgga
     3721 agattattgg tggtgcacgt tgcaagcttt aacttttgaa ggtggaaaag gtccaaattt
     3781 aattgtcgat gatggtggtg atgcaacact tatgattcat acaggtatgg ctgctgaaaa
     3841 agatgcaaat attttggaaa aacttccaga aggagaagac gaaaaggaac tgctggctat
     3901 tttgaaaaaa gttttaaagg agggtaggaa tttctggtcg aaaatgattc ctgaaattcg
     3961 tggcgtttcg gaagaaacaa ctaccggcgt tcatcgtttg tatcaaatgt tggagaaagg
     4021 ttcgttactt ttccctgcct tcaatgtcaa cgactcggtt accaaatcga aattcgataa
     4081 tttgtatggt tgtcgcgaat cattggctga cgggattaaa agagcaacag atattatgct
     4141 ggctggtaaa gtagtagtag tttgcggtta tggcgatgta ggaaaaggtt gtgccaaatc
     4201 aatgcgagct tacggtgccc gagtgctggt aactgaaatt gatccgattt gtgctttaca
     4261 ggctgctatg gaaggatttg aagtttgcac ggtggaagac gctttaccgg aaggaaatat
     4321 ttatgttact gctactggca atcgtgatgt aattactatt cagcatctgg aaaaaatgaa
     4381 agatggagcc attgtctgca atatcggaca tttcgataat gaaattcagg tagatcaatt
     4441 aaaaaaattt ccgggcattc aaaaagtaaa tatcaaacca caagtggaca aatatatttt
     4501 ccctgacggg cacagtattt tgcttcttgc tgacggacgt ttggtaaatc ttggctgtgc
     4561 aacaggtcat ccttctttcg tgatgagcaa ttcttttacc aaccagacgc tggctcaaat
     4621 tgaactgttt acaaaaaaat acaaggtaaa tgtgtatcgt ctgcccaaac atctggacga
     4681 agaagtagcc cgacttcatt tagaccaatt aggagtaaag cttacccatc ttaccaaaga
     4741 acaggctgaa tatattggcg tgaatccaga aggacctttt aaaccggaac attatcggta
     4801 ttaaattgtt ataataataa actaagggtt cttagtggta atatttttag atttttatct
     4861 tataaactga ataattattt caatatagat tttatcaaat cagttggtaa ttttgaaaaa
     4921 aaattttatc tttgcaacct cattttaaag ttgaacccaa aaatttaaca tcaaaaaatc
     4981 aagaaataaa gcattatgat tatcattcct gtaaaagaag gtgaaaacat cgacaaagca
     5041 ttaaaaaagt acaaacgcaa atttgaaaaa acaggtgttg tcagagaact tcgtcgtcgt
     5101 caaagctttg aaaagccttc tataacaaaa cgtaatcaaa aattacacgc tgtttacgta
     5161 caaaaattgc aaagtgaaaa ccaataaaaa ctaccttaaa aagttgtttt ttggtttttt
     5221 aatttacagt cgttgcaaaa ttgaacttat aaggtcatga taaaagattt tttagactat
     5281 ttaaagtatg aaaaaaatta ttcttctcat acaattttat catataagac cgatttgtta
     5341 caattttgcg actttttaaa cactactcct gaagcgttaa atgtttctga ggtaacttcg
     5401 atgcaaattc aacaatggat tgcctcaatg atgtcagaaa aaaaatcaaa acgtaccatt
     5461 tcacgtaaaa tttccacttt aaaatctttt tggcattttt tattggaaag aaaagaagtc
     5521 aatcaaaatc caactttaag aattgtttta cccaaagcaa aaaaaccttt gccctctttt
     5581 tttaaaaacg aagaaatgga aaaagctttg gacgaaacat ttttgcttga aaattttgaa
     5641 gcacaacggg atcatttgat cttagaaatt ttttatttaa caggcatacg tttatcagaa
     5701 cttatttcta ttcaggatag tgatattgat ttgaaggaag gcattttgcg tgtgatcggg
     5761 aaacgcaaca aagaacgaat gattcccctt tcaaaaaaat tatgtgaaga tattgaacat
     5821 tatattcaac tgcgtgacca gcaaatagaa aaaaaaggta attggctttt tgtacgaaaa
     5881 aatggtgaaa aattatatcc aaaatgggta tataataaaa ttcatcgaac gatgtcagat
     5941 gtcagtacct taaataagaa aagtccacat gtattgcgac atagttttgc caccgcttta
     6001 ctcaatgaag gtgccgatat taatgctgta aaagaattgt taggacacag cagtcttgca
     6061 gctacgcaag tttatactca cacaagtttt aaagaattat ataacatata caaacaaacc
     6121 catccaagag cgaaataaaa gaagttaaat tatgaaatta agaatccaag ctattaattt
     6181 tgaggcaacc tcagcattag aatcgtatgt aaataaaaaa ttggcaaagt tagaaaagct
     6241 aacagatgat attttcaatg tggaagtata tttaaagctt ttaaaatcaa gtacgggtgc
     6301 caataaagaa gtagaaataa aagtttccat tccaagtgta gagttttttt catctaaagt
     6361 gtgtgaaact tttgaagaag ctactgactt gtgtattgat gctatcgaaa aacaaattaa
     6421 aaagcacaag gaaaagttca attacaaaaa ataattgatt gtagtttttg gaattcaaaa
     6481 ttatttatgt aaatttgcaa gccgtttaag taggtttatt taaacggctt tttaaacagt
     6541 aaaagaggat attataagta attcgcctct ttagctcagt tggccagagc acgtgatttg
     6601 taatctcggg gtcgtaggtt caaatcctac aagaggctca aagttattta tagtgagctt
     6661 acaatttaaa agttctttat cattttgggc agttgccaga gtggacaatt ggggctgact
     6721 gtaactcagc tgtctgacga cttcggtggt tcgaatccat cactgcccac tgaggagaaa
     6781 tttattgatc tcattttgag gggaattcaa taaatctttt gcggaagtag ctcagttgac
     6841 agagcattag ccttccaagc tgagggtcgc gggtttgagt cccgtcttcc gctcaaaaaa
     6901 tcaaaaaccg agtatcttag aaaagagaaa agataatgat tttttgctgt tgtagctcag
     6961 gggtagagca cttccttggt aaggaagagg tcgcgggttc aactcccatc aacagctcat
     7021 aaaataaaat tttttataac ttataaaatt acttaatttt taaaacaaat ttaaactatg
     7081 gcaaaagaaa aattcgacag gtcaaagcct catgtaaaca taggtacgat aggacatgtt
     7141 gaccatggaa aaaccacttt gactgctgct attaccaaag tacttgctga aaaaggactt
     7201 tctgaagttc gtaccttcga ttctatcgat aatgcaccag aagaaaaaga acgtggtatt
     7261 accattaata cttctcacgt agaatatcaa acagaaaaaa gacattatgc tcatgtagat
     7321 tgtccaggac atgcagacta tgtaaaaaac atggtaacag gtgctgctca aatggacggt
     7381 gctatcgttg tggtagccgc tactgatgga ccaatgcctc aaacacgtga acatatccta
     7441 ttggctcgtc aagtaaatgt tcctcgttta gttgttttta ttaacaaatg tgaccttgta
     7501 gatgatgagg aaatgattga tttagttgaa atggaaatgc gtgaattact taatttctat
     7561 gattttgatg gtgataacac tcctgtcatt cgtggctctg ccttaggagc actcgatgga
     7621 gacccaaaat gggaagccaa aattgaagag ttaatggatg ccgttgatac ttggattcaa
     7681 ttacc
//
LOCUS       DAOSAS010000085         7677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6149_length_7677_cov_14.277355, whole genome shotgun sequence.
ACCESSION   DAOSAS010000085 DAOSAS010000000
VERSION     DAOSAS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6149_length_7677_cov_14.277355"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..946)
                     /locus_tag="PLR72_05075"
     CDS             complement(2..946)
                     /locus_tag="PLR72_05075"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOK36606.1"
                     /translation="MVISDSSFKVFSGTNSRYLAEKICKFLACPLGKMNTEFFSDGEF
                     SVTYEESIRGQNVYLVQSTFPNANNLMELLLMIDAAKRASAYKIIAVIPYFGWARQDR
                     KDKPRVSLGAKLIADMLSVAGINRLITMDLHADQIQGFFDVPVDHLYASTIFAPYIES
                     LNLPNLAIATPDVGGSKRAGSYSKYLDVPMVLCHKTREKANVVDEMRIIGDVIGKDVV
                     IVDDIIDTGGTLCMAANLMMEKGANSVRAIVSHGVMSGSASEKVSKSALVELAFTDSI
                     PYDTTKCPKVKVLSVAEMFADTICRVQEHKSISEQYIF"
     gene            1394..4084
                     /locus_tag="PLR72_05080"
     CDS             1394..4084
                     /locus_tag="PLR72_05080"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOK36607.1"
                     /translation="MIIHFKIPYFTHWGQQIYVKGNIPELGNNETDKGLLLHFTFPEN
                     WIGEVEIVGNKPIEFSYKYFLYDENVNEWKEEWIEDRNIALDPSKSDHFFCFDFWNNP
                     GSIENVFYTAPFKNVFFRQNISLNLSSPQDYTHIFRIKVPLLQEGQVPCLIGDCKKLR
                     NWNIQQPLLLQKTDGNWWKVEVDLNEEKNEIHYKYGIYDTKNNRFLFFESGYDRKAPT
                     FHSNRTCIRLSDGFLRMNVQTWKGAGVSIPVFSIRTKKSFGVGDFLDLKLLVDWAKKI
                     GLKLIQVLPLNDTIGTHTDADILPYAAISAFALNPLFLNLLAIGELPENHPLQKEYKK
                     LQPALNDKEWIDYLTVINFKLKYAKELFIIQKEEFFKKPDYQTFFVQNKHWLIPYAAF
                     CVLRDQFETPDYTQWGDYAKYDAEKIANFTSPSQPHYQEIAYYYFLQYYLHKQLVEAT
                     QYAHEHGIVLKGDIPIGVNRYSVETWFHPEYFNMNMQAGAPPDLFSIKGQNWELPTYN
                     WEAIKQSKYEWWINRLYQMNYYFDAFRIDHILGFFRIWQIPLNQVEGIMGHLYPSIPI
                     YINEFNEKGIWFDYNRFCKPFINDEILGRFFGDDAGWVRASCLQIENGWNFQLKPEFQ
                     SQKAVQKMFEEGKFSEKIKCGLFDLISNVLFFEVEGSNGTQFYPRWGMHYLPSYLYLD
                     DYTKQKLDELYIDYFYRRQDDFWYYSGIKKLSVLKHATNMLICGEDLGTLTPCVTRAM
                     KELGILSLEIQRAPKTDKIEFLHPNDAPYLSVITPSTHDMSTIRGWWEENREVTQRFY
                     NYQLGHWGEAPYFCEPWICREILLQHLYSPAMWAIFQIQDLLSISEKLRRNNPHDERI
                     NVPTNSKQSWRYRFHLNLEDLLEEDDFNEELKNYIIQSGR"
     gene            complement(4150..4231)
                     /locus_tag="PLR72_05085"
     tRNA            complement(4150..4231)
                     /locus_tag="PLR72_05085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4195..4197),aa:Leu,seq:tag)
     gene            complement(4329..5222)
                     /locus_tag="PLR72_05090"
     CDS             complement(4329..5222)
                     /locus_tag="PLR72_05090"
                     /inference="COORDINATES: protein motif:HMM:NF026169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HOK36608.1"
                     /translation="MISDLNDSFLGKKVPIVENYDPSLLFKIPRSINREKYNISSENL
                     PFVGADAWNCYEVSFLTNNGLPISRVMKLIYSANSTYLVESKSLKLYLNSFNMEKFGE
                     TIEESEEMATERIKNDLSNLIGEEVTVALFSAKDKAVKPFSKFYSYDLLQLIPPEISA
                     KIHFEHLVENPNLLRSNLTETTQEYIFYSDILRSNCPVTHQPDWGDVFVKMTTKHTID
                     FSSILQYLVSFRKETHFHEEIVEIIYKRFLEHFQPEKLMVCAMYTRRGGIDINPIRAS
                     ENSLLNEDLLSMQYRVERNFR"
     gene            complement(5244..7022)
                     /locus_tag="PLR72_05095"
     CDS             complement(5244..7022)
                     /locus_tag="PLR72_05095"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOK36609.1"
                     /translation="MEKVLKRISLLRQLMQENKIKAYIIPSTDPHASEYVADYWKERE
                     WISGFDGSAGTVVITMDKAALWTDSRYFLQAEEQLKGTGIELMREGLTETPGIITWII
                     SQLNNNEKVGINPQMISCNAYSSMKEELKMNNLDLVSVDLISKIWTDRPSLPIDPFYE
                     YDIKFAGKSATEKISSVRQQMKQINAEVFLLSALDEIAWLFNIRGNDVAYNPVVIAYG
                     VIELDRAILFVNPLKVTTESSKYLKSEGIIVEPYEEIYSYLKQLAPQQSIWMDGAKLN
                     QSVYEAIPAHCKIINKTSPVIYMKSIKNETEIAGIKQAMIKDGIALIRFFKWLEENVE
                     KNQLTELSVSEQLHHFRNEQENFKGESFETIVGYASHGAIVHYQPTPETNFSIKPKNL
                     LLIDSGGQYLEGTTDITRTISLGNPTNQQKKDFTLVLKGHIELAQVKFPKGTRGSQID
                     IMARQFLWKEGINYGHGTGHGVGHFLCVHEGPQNIRIDENPTALEPGMLISNEPGVYR
                     ANEYGIRTENLVLVTFAEKTDFGEFYQFETLSLCPIDKALIDRNILTENEINWLNNYH
                     QKVFEILSPFLNEEERKWFQQKCLPL"
     gene            complement(7159..7635)
                     /locus_tag="PLR72_05100"
     CDS             complement(7159..7635)
                     /locus_tag="PLR72_05100"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HOK36610.1"
                     /translation="MKNHKINLMLIGILLLASCSTSKVTTESIQALNKKIESKNYTIG
                     VQYAYPLRGKQIYLNANYELKIKNDSAFAFLPYFGVAYTAPYSSTEGGIKFSEPMMDY
                     KAQINQKKKCWEVSFKVNTTFYNYNFDMNIFDNGKVSFTVISNQRDPISFSGEIKN"
BASE COUNT         2568 a         1321 c         1167 g         2621 t
ORIGIN      
        1 cctaaaaaat gtactgttca ctaatagatt tgtgctcttg aactcgacaa atggtgtcgg
       61 caaacatttc tgctacagac aatactttta cttttggaca tttagttgta tcataaggaa
      121 tagaatctgt aaaagccaac tctactaaag ctgacttgct aactttttcg gatgctgagc
      181 cagacataac tccatgcgat acaatagccc gtacactatt agcacctttt tccatcatta
      241 aattggcagc catacacaaa gtgcctcccg tatctattat gtcatcaact attactacat
      301 ctttgcctat tacatcacca atgattctca tttcatccac tacattggcc ttttcacgag
      361 ttttgtgaca cagcaccatc ggtacatcca aatattttga atacgagcct gctctttttg
      421 atcctcctac atcaggagta gctattgcca aatttggcaa attcaacgac tcaatgtaag
      481 gagcgaaaat agtagaagca tacaaatgat ccactggtac atcaaaaaaa ccttgaatct
      541 gatcagcatg taaatccatc gtaattaatc gattaatccc agcaacactt aacatatcag
      601 caataagttt tgctcctaat gatacacgag gtttgtcctt cctgtcttga cgagcccagc
      661 caaaataagg aatcacagca ataattttat aagcagatgc tcgtttagca gcatcaatca
      721 ttaaaagcaa ttccattaaa ttatttgcat tagggaaagt ggactgtacc aaataaacat
      781 tttgacctcg aatggactct tcataagtaa ctgaaaattc tccatctgaa aaaaattcag
      841 tgttcatttt tcccaaaggg caagctaaaa acttgcaaat cttctcagca agatatctgc
      901 tgtttgtccc tgaaaatact ttaaaagatg aatcagaaat taccataatt ttatgtagat
      961 aaaagaattt tttacaaaat tacagaaatt aaaataaaaa attctctatt ggaacaaaat
     1021 tatttccaaa atacttcttt gccattaaaa agttggttta aactctcatt aaaatgtttc
     1081 aaaggtttcg attatctcat aatttattga ttctaattta aacaaaactg ggaatcaaca
     1141 taatatcaac ttctgtatag gggtattaaa tttcaaatgc tcaactctgc tgttcaaatt
     1201 gttttatgca aacgtttgaa ctaattttta tgattttttt taaagtttca atactccttg
     1261 gtaaaaatta aatgtatttt tgataaaata ttttctcgct ctttgttatt taaatttttg
     1321 aaaaacaatt tattgttata atagaattaa tttaattttg aagaaagagt tcaatgcaac
     1381 ataacaaaat catatgatca tacattttaa aataccctat tttactcatt gggggcaaca
     1441 aatttatgta aaaggaaata tccctgaatt agggaacaat gaaacagata aagggctttt
     1501 attacatttc acttttcccg aaaattggat aggagaagta gaaatagttg gaaataaacc
     1561 cattgagttt tcttataagt attttttata tgacgaaaac gttaacgaat ggaaagaaga
     1621 atggatagaa gatcgaaaca ttgcccttga tccttccaaa tcagatcatt ttttttgttt
     1681 tgacttttgg aataatccag gtagtataga aaatgtattt tatacagcac cttttaaaaa
     1741 tgtgtttttt cgacaaaata tttctcttaa tctttcttct ccacaagatt atacccatat
     1801 ttttagaata aaagttcctt tgctacaaga aggtcaagta ccgtgtttga taggagactg
     1861 taagaaatta cgaaattgga atatccaaca accattattg ttacaaaaaa ctgacggaaa
     1921 ttggtggaaa gtcgaagtag atttaaatga ggaaaagaat gaaatccatt ataaatatgg
     1981 aatttacgat accaaaaaca atcgttttct attttttgaa tcaggttacg atcgaaaagc
     2041 tcctaccttt cactcaaaca gaacatgtat tcgactttca gatggttttt taagaatgaa
     2101 tgttcaaaca tggaaaggag ccggtgtttc tattccggtt tttagtatca gaactaaaaa
     2161 aagttttggt gtgggtgatt ttctggattt aaaactgctg gtggattggg ctaaaaaaat
     2221 tggattgaaa ttaattcagg tcctgccact taatgataca attggcactc ataccgatgc
     2281 agatattttg ccctatgcag ctatctcagc ttttgctctt aatccactct ttttaaattt
     2341 attagccatt ggcgaacttc ccgaaaatca tcctttgcaa aaagaatata agaaactaca
     2401 acctgcttta aatgacaaag aatggattga ttatctgaca gtgataaatt ttaaattaaa
     2461 atatgcaaaa gaacttttta ttatacaaaa agaagaattt tttaaaaaac ctgattatca
     2521 aacatttttt gttcaaaata aacattggtt gattccatat gcagcttttt gcgtacttcg
     2581 tgatcaattt gaaacacctg attatacaca atggggagat tatgcaaaat atgatgctga
     2641 aaagattgct aattttactt ctccatctca acctcattat caagaaattg cttattatta
     2701 ttttttacaa tattatttgc ataaacagtt ggttgaagcc actcaatatg cacacgagca
     2761 tggcattgtt ttgaaaggag atatcccaat tggtgtaaac cgttacagcg tagaaacttg
     2821 gtttcaccct gaatatttca atatgaacat gcaagctggc gctccaccag acttgttttc
     2881 aatcaaaggt caaaattggg aattgccaac ctataattgg gaagccatca aacaatcaaa
     2941 atatgaatgg tggattaacc gtctttatca aatgaattat tattttgatg cctttcgtat
     3001 tgatcatatt cttggttttt tccgtatttg gcaaattcct cttaatcagg tagaaggaat
     3061 tatgggacat ttgtatcctt ccattcccat ttacatcaat gaatttaacg aaaaaggcat
     3121 ttggtttgac tacaaccgtt tttgtaaacc ttttatcaat gatgaaattt taggaagatt
     3181 ttttggtgat gatgcaggtt gggtaagagc aagttgccta caaatagaaa atggttggaa
     3241 ttttcagtta aagcctgaat ttcaaagcca aaaagcggtt cagaaaatgt ttgaagaagg
     3301 aaaattttct gaaaaaatta aatgtggctt gttcgattta atttcaaatg ttttgttttt
     3361 tgaagtggaa ggttctaatg gtacacaatt ctatcctcgc tggggaatgc actatctgcc
     3421 ttcttatcta tatcttgatg attataccaa acaaaaactg gatgaattat atattgatta
     3481 tttctatcga cgacaggacg atttttggta ttattctgga ataaaaaaat taagtgtact
     3541 taaacatgct accaacatgc taatttgtgg tgaagacctg ggtacactta ctccttgtgt
     3601 taccagagca atgaaagagc ttggtattct tagcttggaa attcaacgcg caccaaaaac
     3661 tgataaaatt gaatttcttc atccaaatga tgctccttat ttatcagtta tcacgccttc
     3721 tacacatgat atgagtacca ttcgcggatg gtgggaagaa aatcgtgaag ttacccagcg
     3781 attttacaat taccaattag gtcactgggg agaagcccct tatttctgcg aaccgtggat
     3841 ttgtagagaa attcttttgc aacatctgta ttctcccgct atgtgggcta tttttcaaat
     3901 tcaagatttg ttaagcatct cagaaaaatt acgtagaaac aatccgcacg atgaacgaat
     3961 aaatgtaccc accaattcta aacaaagttg gcgatatcgt ttccatctaa atcttgaaga
     4021 tttgctggaa gaagatgatt ttaatgaaga attaaaaaat tatattattc aatcaggtcg
     4081 ataaaaatca atgtcagact ttaaaaacaa agtctttcaa gccataaaaa acttaaaaga
     4141 ctttttttgt gcggctgaag ggactcgaac ccccacgcct ttcggcacca gatcctaagt
     4201 ctggcgcggc taccaattac gccacaaccg caaaatttat ttcctaaatc tttttacaaa
     4261 gataaatctt tccctcttgt tttccaaaca attccaataa tgattggact tttaatgatg
     4321 attagaattt acctgaaatt cctttctacc cgatattgca tgcttaataa atcttcattc
     4381 aacaaactat tttccgatgc tcgaatggga ttaatatcaa ttcctcctct tcgagtatac
     4441 attgcacata ccatcagctt ttctggctga aaatgttcta aaaaacgttt ataaataatt
     4501 tcgacaattt cttcgtgaaa atgggtttct tttctgaatg aaaccaaata ctgcaaaata
     4561 gaagaaaaat caattgtatg ttttgtcgtc attttcacaa acacatctcc ccaatcaggt
     4621 tgatgtgtca ctggacaatt tgatcgcaaa atatcagaat aaaagatata ctcttgagtt
     4681 gtttctgtta aattacttcg aagcaaattg ggattttcaa caagatgctc aaaatgaatt
     4741 ttggctgata tttcaggagg tattaattgg agcaaatcat aagagtaaaa ttttgaaaac
     4801 ggtttcacag ctttatcttt tgctgaaaat aaagctactg ttacttcttc accaattaaa
     4861 ttactgagat catttttaat cctttctgtt gccatttctt cagattcctc aatcgtttca
     4921 ccaaattttt ccatgttaaa ggagttcaaa tacaatttaa gtgacttaga ttcgaccaaa
     4981 taagtactgt tggctgaata gatcaatttc attacacgag aaattggtaa accattattt
     5041 gtcaaaaagg aaacttcata acaattccat gcatctgctc cgacaaaagg taaattttca
     5101 ctggaaatat tatatttctc ccgattgatg gaacgaggaa ttttgaacaa caaagaagga
     5161 tcataatttt ccacaatggg aactttcttc cctaaaaaag agtcattcaa atctgatatc
     5221 ataaatttat acaaatcagt gtattataaa ggaagacatt tttgttggaa ccattttctt
     5281 tcttcttcat taagaaaagg agataaaatt tcaaacactt tttgatgata attattcaac
     5341 caattaattt cattttcagt aagtatattt ctgtcaatta aagccttatc aattggacaa
     5401 aggctcaatg tttcgaactg gtaaaattcg ccaaaatcgg ttttttctgc aaaagtaaca
     5461 agtactaaat tttcagttcg aatgccatat tcattggctc gataaacgcc tggttcgttt
     5521 gaaattaaca ttccaggctc caatgcggta ggattttcat ctattcgtat attttgaggt
     5581 ccttcgtgca cacacaaaaa gtgtcccact ccatgacctg ttccatgtcc gtaatttatt
     5641 ccttctttcc agagaaactg tcgagccata atatctattt gtgaacctcg tgtacctttt
     5701 ggaaacttta cttgagccaa ttcaatatgt cctttcaaaa ccagcgtaaa atcctttttt
     5761 tgttgattcg taggatttcc taaagaaata gttcgtgtaa tatctgtagt tccttctaaa
     5821 tattgtcctc cggaatcaat gagcaataaa tttttcggtt ttatagaaaa atttgtttct
     5881 ggagttggtt gataatgcac aatagctcca tgagaagcat aacctactat ggtttcaaaa
     5941 ctttcacctt taaaattttc ctgttcattt ctaaaatgat gtaattgctc tgaaacagaa
     6001 agctcagtaa gttgattttt ttcaacattt tcttctaacc atttaaagaa tctaatcaaa
     6061 gcaatcccat cttttatcat agcttgcttg atgcctgcta tttctgtttc attttttata
     6121 cttttcatgt aaattacagg agaagtttta ttgattattt tacaatgagc aggaatagcc
     6181 tcataaacag attgattcaa ttttgcacca tccatccaga tagattgttg aggagctaat
     6241 tgtttcaaat aagaataaat ttcttcataa ggttcgacaa ttattccttc actttttaaa
     6301 tactttgatg attcggtagt tactttcaaa gggttaacaa acaaaatggc acgatctaat
     6361 tctattactc cataagctat tactacagga ttgtaagcaa catcattccc tcgaatattg
     6421 aacaaccatg caatttcatc cagagctgaa agaaggaaaa cttcggcatt gatttgtttc
     6481 atttgctgtc taactgaact tattttctcc gtggctgatt ttcctgcgaa ctttatatca
     6541 tattcataaa aaggatctat aggaagagaa ggcctatctg tccaaatttt acttattaaa
     6601 tccaccgaaa ccaggtcaag attattcatt tttaattctt ccttcattga tgaataagca
     6661 ttgcaggaaa tcatttgcgg gtttattcca actttttcat tattattaag ttgagaaatt
     6721 atccaggtta ttattcccgg cgtttcggtt aaaccttctc tcatcaattc aattcctgtt
     6781 ccttttaact gttcttcagc ttgtaaaaaa taacgagaat ccgtccacaa ggcagctttg
     6841 tccatagtaa ttaccactgt gccagcagaa ccatcaaaac cagatatcca ttcacgttct
     6901 ttccaataat cagctacata ctcacttgca tgtggatcgg tgctgggtat aatataagcc
     6961 ttaattttat tttcttgcat taattgacga agcaacgaaa ttcgttttaa tactttttcc
     7021 atagcaaaaa taatttgaag aaataaattt taattgagaa ggagagaaaa tataaagatg
     7081 taaacctaaa gaaaggctga attcaaccct tatgaatttt cgatttataa ataatccatg
     7141 tatactggca gcttattttt agttttttat ttcaccacta aatgaaatag gatctcgttg
     7201 attagagata acagtaaatg agacttttcc attatcaaaa atattcatat cgaaattata
     7261 gttataaaaa gtggtattca ctttaaacga tacttcccag catttctttt tctgattgat
     7321 ctgagcttta taatccatca taggttctga aaattttata cctccttcag tcgagctata
     7381 aggtgctgtg taagctacac caaaataggg taaaaaagca aacgcagaat cattttttat
     7441 tttcaattca taattagcat tcaaataaat ttgttttccc cttaaaggat aagcatattg
     7501 aacacctatt gtgtaatttt tcgattcaat ttttttattc aatgcttgta tgctttcagt
     7561 agtaactttt gatgtagaac aggaagctaa taaaagtata ccgattagca taaggttaat
     7621 tttgtgattt ttcatagacc gtatttttaa aaagttatta atcaaagttt cgcattt
//
LOCUS       DAOSAS010000086         7641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6192_length_7641_cov_12.571579, whole genome shotgun sequence.
ACCESSION   DAOSAS010000086 DAOSAS010000000
VERSION     DAOSAS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6192_length_7641_cov_12.571579"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1541)
                     /gene="rodA"
                     /locus_tag="PLR72_05105"
     CDS             complement(90..1541)
                     /gene="rodA"
                     /locus_tag="PLR72_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOK36611.1"
                     /translation="MVRSSIKYALDWRTIFYYVILVIMGWLSVYAASFDFDHPGILDF
                     SQRSGKQFIWILSAFGLAGIILLIDSRIYNYFSYLIYGLTLLLLVVTIFIAPNIKGSR
                     SWIVIGPVNFQPAELAKMATSLALAKFMSGYNYKIKTWKDLIPLLVIIGIPLVLILLQ
                     KETGSALVFLSFFLVFYREGMKGIVLLLGIFAIVLFVLVIRFSMVPIQGDKGTLGIIL
                     AMGLILLVQFVYSFFIKKNKKEALFFLAGIVAVFFISYLLSLWIDINYNAVAFFTVIA
                     SCIYWAIVEIIHKHKQYLFLAIVSLCAALFSASSDYLFENVLEPHQKVRIKVLLNMES
                     DPLGAGYNINQAKIAIGSGGFTGKGFLNGTQTKLQYVPEQDTDFIFCTVGEEGGFLGS
                     VFVLIIYWLFLMRLITIAERQRDTFNRVYGYCVVSIFFFHLIVNIGMVLGIMPIIGIP
                     LPFFSYGGSSLWGFTILLFILLRLDASRIEHVR"
     gene            complement(1528..3363)
                     /locus_tag="PLR72_05110"
     CDS             complement(1528..3363)
                     /locus_tag="PLR72_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOK36612.1"
                     /translation="MVNDPFQNRRHIISIIISVFIGIFIIRLFSLQVIETKYKKGADS
                     NAFLNKTLYPPRGLIYDRNHNLLVYNKPAYDLMLIVREIHDLDTTAFCEALEINKQFF
                     IQRMAEIKDRKKNRGYSSYTPQVFMTQLDIKDIANIQQQLYKFPGFYIQNRTLRDYRY
                     PNAAHVLGSIGEVSQAMIDKDDYYKIGDYAGRDGLEYTYEKELRGEKGVEILLRDAKG
                     RIKGKYDNGKHDVPPKAGKNLITTLDIQLQIVGEQLLTGKIGGIVAIEPSTGEILAMV
                     SSPTFDPSSLVGRQRTQNYAKLVNDPTKPLLNRATQAQYSPGSTFKTIQALVCQQMGG
                     IDTNTLFPCNGPATTPIKCTHFHGSPVNLLKGIEQSCNPYFWGAFHTTLELGGYGEKN
                     VNFKKNYNTWRKHVLSFGFSQKFTDSDIYQQSSGNIPTEKYYNHYFGETGWRAITIRS
                     LAIGQGEILVTPLQLANAMAIIANKGYYITPHLNKSDSMLVRTHFTTVDKKYFNVVDE
                     GMWRVCEYGTARHYKIPNMAMCGKTGTVQNPFGKDHSIFVGYAPRENPKIAIAVVVEN
                     AGFGATWASPIANLMIEQYLFGKINRVDLFNRISNSYFISSYGQE"
     gene            complement(3366..3863)
                     /gene="mreD"
                     /locus_tag="PLR72_05115"
     CDS             complement(3366..3863)
                     /gene="mreD"
                     /locus_tag="PLR72_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOK36613.1"
                     /translation="MTLALQNIIRFVLLVLAQVLILNNIQFLGYINPYIYILFIFSLP
                     YRTPQWLILSLAFILGLTIDIFSNTLGMHSFATVLMAFTRNGVIQLFTSFEKEENPIP
                     SFRTFGISAYLKFIIVMVLIHHTTLFLLEAFSFSNFFFIFKKILLSSMISIVLILGIQ
                     SFKKK"
     gene            complement(3864..4703)
                     /gene="mreC"
                     /locus_tag="PLR72_05120"
     CDS             complement(3864..4703)
                     /gene="mreC"
                     /locus_tag="PLR72_05120"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOK36614.1"
                     /translation="MKNLINFLIHHSGLLLFIILEIISFIMMINSQEYQKSIFLSSSN
                     AVVATLYNWTNSVGEFFKLRNTNNQLSSENTELKNQIIELQNKLKVLEPSITSLNFKL
                     LPENEYRFISAKVINNSTDKFQNYLTLNKGSKDGVKPDMGVVSSEGVVGIIKSVSPRF
                     SVVIPILNPKIQISCKFKRTDYTGSLVWNGEDYRYANLVDIARHVDIRLGDTIVTSGF
                     TRTFPEGIPVGIVEDYSIKESDAYYNIKVKLAVNFRTLTYVNIIDYLNYQEQKQLEEK
                     NYE"
     gene            complement(4803..5822)
                     /locus_tag="PLR72_05125"
     CDS             complement(4803..5822)
                     /locus_tag="PLR72_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOK36615.1"
                     /translation="MGLFSFTQEIAIDLGTANSVITYNDQIVVDEPSVVALDKRTDRL
                     IAVGERARQMQGKENEAIRTIRPLRDGVIADFYAAEMMIRGLIKMIPTKNHFFSPSLR
                     MVVGIPSGSTEVEIRAVRDSSEHAGGREVYMIYEPMAAALGIGIDVEAPNGCMIVDIG
                     GGTTEIAVISLGGIVLNKSIRIAGDDLTMDIIEYMGRMHNIKIGERTAELIKINVGAA
                     LTDLEDGPEDYIVRGPNRMTALPIEVPVSYQEIAHCLEKTISKIEAAVLSALEETPPE
                     LYSDIVKQGIYLCGGGALLRGMDKRLTEKINIPFQIADDPLHAVARGTGVALKNIDKF
                     SFLLR"
     gene            complement(5874..7394)
                     /gene="purH"
                     /locus_tag="PLR72_05130"
     CDS             complement(5874..7394)
                     /gene="purH"
                     /locus_tag="PLR72_05130"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOK36616.1"
                     /translation="MEEKRIKKALISVYHKDHLEDIIIKLHSEGVALISTGGTKKFIE
                     SLNLPCQSVEELTGYPSILGGRVKTLHPKIFGGILARRDIEEDLEQLKQYEIPEIDLV
                     IVDLYPFEATVNAGANEPTVIEKIDIGGMSLIRASAKNFNDVVIIASQAQYEPFMEIL
                     TTKGAQTTLEERRWFAKEAFAVTSQYEIAIFNYFDRSYQSAFRYGENHPKILRYGENP
                     HQQGIFYGKFDEMFEQLQGKEISYNNLLDIDAAVNLINDFDDITFAILKHNNACGIAT
                     RPQLKDAWQAALAGDPVSAFGGILITNAIVDKETAFEINKIFFEVLIAPDYDLEAIEI
                     LSQKKNRIILIMKSTKLKPKQFRSILNGVLTQDRDTHIETINDLKIVTEKSPTAEEIE
                     DLLFANKVVKHSKSNAIVLAKNKQLLGSGVGQTSRVDALKQAIDKAKSFNFDLKGAVM
                     ASDAYFPFPDCVQLAYEAGITAIIQPGGSIRDKDSIDLCNQNRISMVFTGYRHFKH"
BASE COUNT         2592 a         1349 c         1165 g         2535 t
ORIGIN      
        1 tttttgattt atcgacgact aaatttttcc ctttacaaaa ataaactttt aaagtcagaa
       61 atggttcaga aattttgtaa aaaaggagat taacgtacat gttctatcct tgaagcatct
      121 aatcgaagca aaataaaaag caaaatagta aacccccata aggaggatcc accataacta
      181 aaaaaaggca aaggtattcc tataataggc attattccta ataccattcc aatattgact
      241 attaaatgaa aaaagaaaat actgaccaca caataaccat agactcgatt aaaagtatca
      301 cgttgacgtt cagcaatggt aattaatcgc attaaaaaga gccaataaat aattaataca
      361 aaaacagaac caagaaaccc cccttcttct ccaacggtac agaaaataaa gtccgtatct
      421 tgttcgggaa catattgtaa tttagtttgt gtcccgttta aaaaaccttt tcctgtaaaa
      481 cctcccgaac caatagcaat ttttgcttga ttaatattat atccggctcc aagtggatca
      541 ctttccatat tcaacaaaac cttgatgcga actttttgat gaggttccaa tacattttca
      601 aataaataat cggaagaggc actaaataaa gcagcacaaa gtgaaacaat tgctaaaaaa
      661 agatattgtt tatgtttatg aattatttca acgatagccc aataaataca ggaagcaatt
      721 accgtaaaaa atgcgacagc attgtagttt atatctatcc ataaactaag cagataagat
      781 ataaaaaaaa ctgctacaat gcctgccaag aaaaataaag cctctttttt atttttttta
      841 atgaaaaaag aataaacaaa ttgaaccaac aaaatcaatc ccatcgctaa aataattccc
      901 agcgtacctt tatcaccttg aataggcacc atgctaaagc gaatgactaa aacaaataaa
      961 acaatagcaa aaattccaag caacaagaca attcctttca ttccttcacg ataaaaaaca
     1021 aggaaaaaag aaagaaaaac taaggcagaa ccggtttctt tttgtaataa aataagcacc
     1081 aatggaattc caataataac taaaagaggt attaaatctt tccatgtttt gattttatag
     1141 ttatagccac tcataaattt ggctaacgcc agcgaggtag ccattttagc taattcagca
     1201 ggttgaaaat tgacaggacc tatcactatc cacgaacgag aacctttgat attaggagca
     1261 ataaaaattg ttacaactaa taataataaa gtaagtccat aaattaggta gctgaaataa
     1321 ttatatattc tcgaatcaat aagcaaaata attccagcca gaccaaaagc agagagaatc
     1381 cagataaatt gttttcctga gcgttggcta aaatcgagaa tgccaggatg gtcaaagtca
     1441 aagcttgccg catagacact taaccaaccc attattacca atatcacata atagaatatg
     1501 gttcgccaat ccaaagcata tttaatacta ctcctgacca tacgaagata taaagtatga
     1561 atttgaaatt ctgttgaata aatccactct atttattttc ccaaacaaat attgttcaat
     1621 cattaaattt gcaatcggag atgcccacgt agctccaaat cctgcatttt caacaaccac
     1681 agcaatagcg atttttggat tttctcgcgg ggcataacct acaaaaattg aatgatcttt
     1741 cccaaaagga ttttgtactg tccctgtttt gccacacatt gccatattcg ggattttata
     1801 atgtcgtgct gtcccatatt cacaaactct ccacattcct tcatctacca cattaaaata
     1861 ttttttatct actgttgtaa aatgggtcct tactaacata ctgtctgatt tgttgagatg
     1921 aggtgttata taatagcctt tattggctat tattgccatc gcatttgcca gttgcaatgg
     1981 tgtgaccagt atttcacctt gtccaatggc taatgaacgg atagtaattg ctctccaccc
     2041 tgtttcacca aaataatgat tgtaatattt ttcggtagga atatttcctg atgattgttg
     2101 ataaatatca ctatcagtaa atttttgact aaatccgaaa ctcaaaacat gttttcgcca
     2161 agtattataa ttttttttga aattgacatt tttttctcca taacctccta attcaagcgt
     2221 tgtgtgaaaa gctccccaga aataaggatt gcaactttgt tcaatacctt ttaataagtt
     2281 tactggtgaa ccgtgaaaat gggtacattt aataggggtg gtggctggtc cattacaagg
     2341 aaataaagta ttggtgtcaa ttccccccat ttgttgacaa actagtgctt gaatagtttt
     2401 aaaagttgaa cctggcgaat attgagcttg tgtagctctg tttaaaagtg gtttagtagg
     2461 atcattgact aattttgcat aattctgagt cctttgtcgt cctactaaag aagaagggtc
     2521 aaaagttgga ctggaaacca ttgccaaaat ttcgcctgtc gatggttcaa tggcaactat
     2581 tccgcctatt ttccctgtta ataattgttc tcctactatt tgaagttgaa tatcaagcgt
     2641 agttattaaa tttttacctg ctttaggtgg aacatcatgt ttgccgttgt cgtattttcc
     2701 ttttattctt cctttggcat cgcgtaaaag aatttctaca cctttttcgc ctcttaattc
     2761 tttttcataa gtatattcaa gcccatctct gcctgcataa tctccaattt tataataatc
     2821 atctttgtca atcatagctt gtgaaacttc accgatacta cccaaaacgt gagctgcatt
     2881 aggatacctg taatctctta atgttctgtt ttgaatgtaa aaaccgggaa atttatacaa
     2941 ttgttgctgg atattggcta tgtcttttat gtccagttgt gtcataaata cctgaggagt
     3001 ataggaagaa taccctcgat ttttcttcct atcttttatt tctgccattc gttggatgaa
     3061 aaattgtttg ttaatttcca aagcttcaca aaaggcagta gtatctaaat catggatttc
     3121 tcgtacaata agcattaaat cgtaagcagg tttattgtaa accagcaaat tatggttccg
     3181 atcataaatc agtcctcgtg gtggatacag ggttttattt aaaaaagcgt tgctatctgc
     3241 tcctttttta tattttgttt cgattacctg caaagaaaac agacggataa taaatatacc
     3301 aatgaatact gatataataa tggaaattat atgccttctg ttttgaaaag gatcgtttac
     3361 catttctatt ttttcttaaa cgattgaatg cctaagatta atacaatact tatcattgag
     3421 ctaaggagaa tttttttaaa gataaagaaa aaattcgaga aggaaaatgc ttctaacaaa
     3481 aacaaagtag tgtgatgaat caataccatt acaataatga attttaagta ggcactgatg
     3541 ccaaaagttc tgaaggatgg aataggattt tcttcttttt caaaggaagt gaacagttga
     3601 ataacaccat ttcttgtaaa agccattaaa accgttgcaa aagagtgcat ccccaaagta
     3661 tttgaaaaaa tatcaattgt taatcccaaa ataaaagcaa gagaaagaat taaccattga
     3721 ggagtacgat aaggcagaga aaaaataaaa agaatataaa tataaggatt aatgtatcct
     3781 aaaaactgaa tattatttaa aatcaatact tgagccaata ccaataaaac aaaacgaata
     3841 atattttgaa gagctaatgt catttattca taattctttt cttctaactg cttttgttct
     3901 tgataattta agtagtcaat gatatttaca taagtaaggg ttcgaaaatt aacagcaagt
     3961 tttactttaa tgttataata agcatcgctc tccttaatac tataatcttc tacaattcca
     4021 acaggaattc cttcagggaa agttctcgta aaaccactgg ttacaatcgt atctcccaat
     4081 ctaatatcta catgtcttgc tatatctact aaattggcat aacgataatc ctctccattc
     4141 caaaccagag aaccagtata atcagttcgt ttaaatttgc aactgatttg aatttttgga
     4201 tttaaaatgg gaataactac agaaaaacga ggagataccg acttaataat tccaactact
     4261 ccttcgctgc taacaacacc catatcgggt ttaactccat cttttgatcc cttattaaga
     4321 gtgagatagt tctggaattt atctgtactg ttattgatta ccttagcaga gatgaatcga
     4381 tattcgtttt caggtaataa tttaaaattt aaagaggtaa tggaaggttc taagactttt
     4441 aatttattct gtaattcaat aatttgattt ttaagttcag tattttctga tgatagttga
     4501 ttgttcgtat ttcgtaattt aaaaaattct cctactgaat tggtccaatt gtaaagagta
     4561 gcaactactg cattactcga agaaagaaaa atactttttt gatattcttg tgaattaatc
     4621 atcataataa aagaaataat ttcaagtatt ataaataata acaaaccact atgatgtatc
     4681 aaaaaattta tgaggttttt cataataaat acaattctgc agggattgtt tttaaaataa
     4741 aaattcagcc aaatgaaaat gaagcattca tcattttaat ttattttgtt gaatttttaa
     4801 atttatcgaa gtaaaaaaga aaatttatcg atatttttta aggcaacacc tgttcctcga
     4861 gccaccgcat gcaaaggatc atcagcaatt tgaaaaggta tattgatttt ttctgttaga
     4921 cgtttgtcca tcccccgaag taatgctcca cctccacata aataaattcc ttgttttacg
     4981 atatctgaat atagttcagg tggggtttct tccagagcac ttaacactgc tgcctcaatt
     5041 ttggaaatcg ttttttcaag acaatgagct atttcttgat aagaaactgg aacttcaata
     5101 ggcaaggcag tcattcgatt gggaccacga acaatgtaat cttctggtcc atcttctaaa
     5161 tcggttaatg ctgcaccaac atttattttg attaactctg ctgtccgttc gccaatttta
     5221 atattatgca ttcgtcccat atattcaatg atatccatag tgagatcatc accagcgata
     5281 cgaatagatt tattcaacac gatgccaccc aacgaaataa ctgcaatttc tgtagtacca
     5341 ccaccaatat ctacaatcat acagccatta ggggcttcta catcaatacc tattcccaaa
     5401 gcagcagcca ttggctcata aatcatataa acttctctac ctcctgcatg ttctgatgaa
     5461 tcacgtacag cacggatttc cacctcagta cttccagagg gaatgcctac taccattcgc
     5521 aaagaaggcg aaaagaaatg atttttagtt ggaatcattt tgataagtcc tcttatcatc
     5581 atttctgctg cataaaaatc agcaattaca ccgtcacgta atggtctaat agtacgaata
     5641 gcttcatttt ctttcccctg catctggcgg gcgcgttcac ctactgctat taatcggtct
     5701 gtacgcttat ccagagcaac aaccgaaggc tcatcaacaa ctatctggtc attatacgta
     5761 ataacactgt ttgcagtacc taaatcgatt gctatttctt gtgtgaatga aaaaagtccc
     5821 attttttata aatgatattt gtatgttaat gatatattgt atttttaaaa aaattaatgt
     5881 ttgaaatgtc ggtagccggt aaataccatt gagattctat tctggttgca taaatctatt
     5941 gaatctttat ctctgataga acctccaggt tgaatgatgg ctgtaatccc tgcttcataa
     6001 gcaagttgta cgcagtcagg gaaaggaaaa taagcatccg acgccattac agctcctttt
     6061 agatcaaaat taaatgattt agctttgtca atagcttgtt tcagtgcatc gactcttgaa
     6121 gtttgtccaa cgccgcttcc taacagttgt ttattttttg ctagaacaat tgcatttgat
     6181 tttgaatgtt ttacaacttt atttgcaaaa agtaaatctt caatttcttc ggcagtaggt
     6241 gatttctcag ttactatttt taaatcattt atagtttcta tatgagtatc tctgtcttga
     6301 gtaagcactc cattgagtat agaacgaaat tgtttgggtt ttaatttcgt acttttcata
     6361 atcagaatga ttcgattttt tttctggctc aaaatttcaa tggcttctaa atcataatca
     6421 ggggctatca acacttcgaa aaaaatctta ttaatttcaa aagcagtttc tttatcaaca
     6481 atggcatttg taattaatat ccctccaaat gctgagacag gatctcctgc taatgctgct
     6541 tgccatgcgt cttttagttg aggacgtgta gcaattccgc aagcattgtt gtgttttaaa
     6601 atagcaaaag taatatcgtc aaaatcatta attaaattta cggccgcatc aatgtccaag
     6661 agattgttat aagaaatttc tttcccttgc aattgttcaa acatttcatc gaattttccg
     6721 tagaaaattc cttgttgatg aggattttca ccatatctga gtattttagg gtgattttcg
     6781 ccatatcgaa aagcgctttg ataagaacgg tcaaaatagt taaaaattgc aatttcgtat
     6841 tgtgaagtca cagcaaaagc ttctttagca aaccagcgac gttcttcaag cgtagtttga
     6901 gctcctttgg ttgtgagaat ttccataaaa ggttcgtatt gagcttggga tgctattatt
     6961 accacatcat taaaattttt tgctgatgct cgaataagcg acataccacc tatgtcgatt
     7021 ttttcaataa cagtaggttc atttgctcca gcatttaccg tagcctcaaa aggatataaa
     7081 tcaactataa ccaaatcaat ctcaggtatt tcatattgct ttaattgttc cagatcttct
     7141 tcaatatccc ttctggcaag tatcccacca aatatctttg gatgaagagt ttttactctt
     7201 cctcctaaaa tggaaggata accagttagt tcttccactg actgacaggg taggtttaaa
     7261 gattcaatga acttttttgt gcctcctgta gaaataagag caactccttc ggaatgtaat
     7321 tttatgataa tatcttctaa atggtctttg tgataaactg atattaaggc ttttttaatt
     7381 cgtttttctt ccattttttt tgtatttaat ttattgacaa agttataaaa aaatagtggt
     7441 tttatattaa ctaaataggg atagataaaa caacaaatta tttttaataa atctacacaa
     7501 aaataaacaa aactcaaatt caatgaattg agaatctata ggttggattg caaatagaat
     7561 gcacctgaat tttgactata agtcaattaa aatgttaaac taaagtttat tgaaattatt
     7621 caagaataaa attctcatct g
//
LOCUS       DAOSAS010000087         7578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6277_length_7578_cov_13.371129, whole genome shotgun sequence.
ACCESSION   DAOSAS010000087 DAOSAS010000000
VERSION     DAOSAS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6277_length_7578_cov_13.371129"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLR72_05135"
     CDS             complement(<1..408)
                     /locus_tag="PLR72_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOK36617.1"
                     /translation="MSKIIINSYEELEQYIGKELGVSDYITITQEQINQFADATLDHQ
                     WIHIDPAKAKTESPYKNTIAHGYLTLSLLPYLWNQIVEVNNSKMTVNYGIDKLRFKEP
                     VLTNSEVRVRAKLKSLIKLKGIAKAEIHTILEIK"
     gene            673..1491
                     /gene="panB"
                     /locus_tag="PLR72_05140"
     CDS             673..1491
                     /gene="panB"
                     /locus_tag="PLR72_05140"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOK36618.1"
                     /translation="MSAINENARKVTTRRLLEMKQRGEKISMLTAYDYTMANIVDEAG
                     IDVVLVGDSASNVICGNATTLPITLDQMIFLAKSVVRGVKRALIVVDMPFGSYQGNSR
                     EAIASAIRIMKETHADGIKMEGGEEILESVHRILSAGIPIMGHLGLMPQSINKYGSYT
                     VRAQEEEEAKKLIRDAHLLEEAGCFSIVLEKIPAKLGAQIASELTIPVIGIGAGGGVD
                     GQVLVIHDMLGLTQGFSPRFLRRYANLREIMHNAIQHYIEDVKSGDFPNENEQY"
     gene            complement(1556..2272)
                     /locus_tag="PLR72_05145"
     CDS             complement(1556..2272)
                     /locus_tag="PLR72_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK36619.1"
                     /translation="MGRAFEYRKATKFKRWGNMARVFTKLGKQITIAVREGGPDPDMN
                     PRLRVLMQQAKKENMPKENIERAIKKAISKDEIDSYKEVLYEGYAPHGIAMIIETATD
                     NPTRTVANIRSYFNRYGGSLSTSGSVQFMFDHKCVFRIAPQINTSLEDLELELIDYGV
                     DEVDQDDDSIVLYGDFPSYYSIQKYLEDHNFEIYSAQFEWIPKDMKELNDDQKAQVIK
                     LIEKIEEDDDVQNIFHNMIE"
     gene            2524..3108
                     /locus_tag="PLR72_05150"
     CDS             2524..3108
                     /locus_tag="PLR72_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOK36620.1"
                     /translation="MEYPTLKEKLIMPEYGRNIQQMISYAMTIEDREERTRCVKTIVN
                     IMGNLFPYLRDVSDFKHKLWDHVAIMSNFQLDIDFPYEILRPDDLHTRPEPIPYKNSQ
                     IRYRHYGRTLEEMIEKVADYPEGEERNELIRLIANQMKKCFLTWNKETVDDRKIFDDL
                     RELSGGKIDISEDFLKLVESREVLHSKKSKQKRK"
     gene            3169..4476
                     /gene="murA"
                     /locus_tag="PLR72_05155"
     CDS             3169..4476
                     /gene="murA"
                     /locus_tag="PLR72_05155"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOK36621.1"
                     /translation="MSSFKIEGDQKLSGTIVPQGSKNEALQVICATLLTSEKVIISNV
                     PHILDVKLLMNLLIQLGVEIEKLQENTYSFKAENIKLNYLKTKDFYQKSSLLRGSVMI
                     VGPMLARFGEAIFPKPGGDKIGRRRLDTHFIGIQKLGAKFMFDEMKNIYQIKAPKLKG
                     SYMLLDEASVTGTANIIMTAVLAEGTTTIYNAACEPYIQQLCKMLIQMGAHIEGVGSN
                     LLTIKGVTSLKGCNHQILPDMIEIGSFIGLTAMTASEITIKNVAYSQLGIIPDSFRRM
                     GIQLVQQGDDIYIPSQENYTIETFMDGSIMTIADAPWPGLTPDLLSVFLVIATKANGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLGNQLNLKASIMTSPDIRAGI
                     ALLIAALSAKGTSVIHNIEQIDRGYQNIDKRLNELGAKILRIN"
     gene            complement(4721..5146)
                     /gene="ssb"
                     /locus_tag="PLR72_05160"
     CDS             complement(4721..5146)
                     /gene="ssb"
                     /locus_tag="PLR72_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOK36622.1"
                     /translation="MSVNKVILIGNVGKDPEIRHLDKNVTVAKFPLATSERGYTMQNG
                     TQVPERTEWHNIVAWRGLAEIVEKFVKKGSQLYVEGKIQSRSWEKDGIKRYITEIYAE
                     NIQLLGKRSEAAEEIATSSTVESVQIPPELEEIDDDLPF"
     gene            complement(5366..5719)
                     /locus_tag="PLR72_05165"
     CDS             complement(5366..5719)
                     /locus_tag="PLR72_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36623.1"
                     /translation="MNGIKKFSILEDEMLNSLVEAIYDSELIYSSVDLYSLGIESPNE
                     IDAAVMSAIQTIRRADLSPQQHFKYIYLTDIKSGNILHDWRMSKMGFLLTLLNANTTN
                     SLINQLKIELINRIV"
     gene            complement(5740..6690)
                     /locus_tag="PLR72_05170"
     CDS             complement(5740..6690)
                     /locus_tag="PLR72_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOK36624.1"
                     /translation="MEYKDYYKILGVNKNASQEEIKKAYRKLAVKYHPDKNPGNKQAE
                     EQFKLINEAYEVLGDPEKRKKYDDLGANWNRFSQEGSYSGGSPFEGFGHQGGQQFFYE
                     GDLNDLFGGGRGSGFSDFFERFFGRGAAGRGASSGFGRGFRQAEVRGQDYEVEIVLTL
                     EEAYHGTSRIIQLDNEKIRIKIKPGAYDGQVLRIKGKGGKGSSPANNGDLLVKIKILP
                     HPVFERIGDDLKIVHQISLFDAILGGKTIVDTLDGKIKIDIPEGTQNNKILRIKGKGM
                     PIYGKTNEYGDLLVQLQIKIPDNLTPAQKNMFKQIKDTIQ"
BASE COUNT         2471 a         1265 c         1117 g         2725 t
ORIGIN      
        1 cttgatttcc aaaatagtat gaatttctgc ttttgcaatt ccttttaact taataagcga
       61 ttttaatttt gctcgtactc ttacttcgct atttgttaat acaggttctt taaaacgtag
      121 tttatcgatc ccatagttga cagtcatttt cgaattgtta acttcaacga tttggttcca
      181 taaataggga agaagagata aagtaagata accatgagca atggtattct tataagggct
      241 ttctgttttt gctttcgccg gatcaatatg aatccactga tgatcaaggg tagcatcagc
      301 aaactgattg atttgttctt gtgtaatagt aatataatct gaaacgccta attcttttcc
      361 tatgtactgt tctaattcct cgtaactatt gataataatt ttactcattc ttcataagtt
      421 ttttatttca atcaattgca aaggtataat ttttttaggt tattcaaata ttttccaaat
      481 agcaaaaaac actattgaat aaaagaaaac actaaaaaat aaaatcattt tcattgttta
      541 aattataagc aaaaaaatta aaaaaatcag attttttgct attctatttt aatattttac
      601 ctatttattt tttaacacta ttctttttta atttttttgt aagtttgtac attattttaa
      661 atcctactaa taatgtctgc aattaatgaa aatgccagaa aagtaactac tcgtcgtttg
      721 ctcgaaatga agcaacgagg agaaaaaatc agcatgctta ctgcttatga ttatactatg
      781 gcaaacattg ttgatgaagc aggaattgat gtagttttag ttggggattc tgcttcaaat
      841 gtaatatgtg ggaatgctac tactttgcct attactttgg atcaaatgat ttttttggct
      901 aaatcagtag tgcgtggtgt aaagcgagct ttgatagtag tagatatgcc ttttggaagt
      961 tatcaaggga attcaagaga agctattgca tcggctattc gcattatgaa agagactcat
     1021 gctgatggca taaaaatgga aggaggtgaa gaaattcttg aatcagttca tcgaatttta
     1081 agtgctggca ttcctattat ggggcatctt ggtttaatgc cacaatctat taataaatat
     1141 ggttcttaca cagtaagagc tcaagaagaa gaagaagcaa aaaaattaat aagagatgct
     1201 cacttacttg aagaagcagg ttgtttttct attgtgctgg aaaaaattcc cgcaaaatta
     1261 ggggctcaaa tagcttcaga acttacaatt cctgtgatag gcataggtgc tggcggagga
     1321 gtagatggtc aggtattggt tatccatgac atgttagggc tgacacaagg attttcgcca
     1381 cgttttcttc gtagatatgc taatttgcga gaaattatgc acaatgccat tcagcattat
     1441 attgaagatg ttaaatctgg cgatttcccc aatgaaaatg aacaatattg aagataaatt
     1501 ttttaaaatc cgaataaaaa ttcaataaaa aatattattt gatgaaacag aaattttatt
     1561 ctatcatatt gtggaaaata ttttggacat catcatcttc ttcaattttt tcaatcaact
     1621 ttattacttg agctttctga tcgtcattta attccttcat gtcttttgga atccattcaa
     1681 attgggcact ataaatttca aaattatggt cttccaaata cttctgaata gaataataag
     1741 aagggaaatc accatataac acaatactgt cgtcatcctg atctacttca tcaacaccat
     1801 aatcaattaa ttctaattcc agatcttcga gagaagtatt aatttgcgga gcaattctaa
     1861 aaacacattt atgatcaaac ataaattgta cagagccaga agtgcttaac gagccaccat
     1921 aacgattaaa ataacttcgg atgtttgcta ctgttcgtgt tggattgtca gttgctgttt
     1981 ctattatcat ggctattcca tgaggggcat aaccctcata aagtacttct ttataactat
     2041 ctatttcatc tttggagatt gcttttttta tcgctctttc gatattttcc ttgggcatgt
     2101 tttccttttt tgcttgctgc atcaaaactc tcaatcttgg attcatgtca ggatcagggc
     2161 caccctcacg gactgctatg gtaatttgtt tccccaattt tgtaaatacc cgggccatat
     2221 taccccaacg tttaaatttc gttgcttttc ggtactcaaa tgctcttccc atacgtaata
     2281 atttatattc gtttacttat tatctgaatc atttatagat gcaaatttag agaatttttc
     2341 caattatgta aaataactct cttcccgaat gattactttt atcatgagtc tttatcactt
     2401 atggaattat gtttacaaaa aatctgaaat caattttaac tacaaaagac aatgattttt
     2461 ttcaatatgc ttttttctca acaaacaatt cattattttt gtcaattata aaacttcttt
     2521 atgatggaat atccaacttt aaaagagaaa ttaatcatgc ctgaatatgg acgaaatatt
     2581 cagcaaatga tttcttatgc aatgactatt gaggatcgag aagaacgtac tcgatgcgta
     2641 aaaacaattg taaacattat gggcaattta ttcccttatt tacgagatgt gagcgatttc
     2701 aaacacaaat tatgggatca tgtagccatt atgtctaatt ttcaactaga tattgatttc
     2761 ccttacgaaa ttttacgacc tgatgattta catactcgtc cggaacccat tccttataaa
     2821 aattctcaaa ttcgttatcg tcattatgga cgtaccttgg aagaaatgat tgagaaagta
     2881 gcagattacc cagaaggaga agaaagaaat gaattaatcc gtctgattgc taatcagatg
     2941 aaaaaatgtt ttcttacgtg gaataaggaa acagtggacg atcgaaaaat ttttgatgat
     3001 ttacgcgaat tatcaggagg aaaaattgat atttcagaag attttcttaa actagttgaa
     3061 tcaagagaag tacttcattc gaaaaaaagt aagcagaaaa ggaaataatt atctgtcatt
     3121 tttcgagatt ataaagtaga aaggtataaa tttagctatc tttattttat gtcttcattc
     3181 aaaattgaag gagatcagaa gctttcaggc accattgttc cacaaggctc caaaaatgaa
     3241 gcattgcagg taatttgtgc tactttacta acttcagaaa aggtgattat tagtaatgtt
     3301 cctcatatat tggatgttaa gttgctaatg aatcttctga ttcaacttgg agttgaaatt
     3361 gagaaactac aagaaaatac ctattccttt aaggctgaaa atataaaatt aaattatctc
     3421 aaaacaaaag acttttatca aaaaagctct ttgcttagag gttctgtaat gatagtagga
     3481 ccgatgttgg ctcggtttgg agaagccatt tttcctaaac cgggtggaga taaaatagga
     3541 agaaggcgat tggatactca ctttattggt atccaaaaat taggagctaa atttatgttt
     3601 gacgaaatga aaaacattta tcaaataaaa gctccaaaat tgaaaggcag ttatatgttg
     3661 ctcgacgaag cttctgttac aggaactgct aacatcataa tgacagcagt tttagctgaa
     3721 ggaaccacta ccatttacaa tgctgcttgc gaaccttata ttcaacagct gtgcaaaatg
     3781 ctcattcaaa tgggcgcaca tatcgaaggc gttggatcaa atttattaac cattaaagga
     3841 gtgacttcgt tgaaaggttg taaccatcag attttgccag acatgattga gattggtagt
     3901 tttatcgggt tgacagcaat gactgcctca gaaatcacca ttaaaaacgt tgcttattcc
     3961 caattaggta ttattcctga tagttttcgt cgtatgggga ttcaactggt acagcaaggt
     4021 gatgatattt atattccttc tcaagaaaac tatacgatcg aaacttttat ggacggttcg
     4081 attatgacca ttgctgatgc accgtggcct ggtttaacac ccgatttgtt aagtgtgttt
     4141 ctggtgattg ctaccaaagc aaacggcagt gtgttaattc atcagaaaat gtttgaaagc
     4201 cgtttgtttt ttgtagataa gttaattgat atgggggctc agattatact gtgtgaccct
     4261 caccgtgcaa ctgttattgg tttaggtaat caattgaatt taaaggcttc aattatgact
     4321 tcgcctgata ttcgagcagg aattgcttta ctaattgcag ctttgtcagc aaaaggaacc
     4381 agtgtaattc ataacattga acagattgat cgtggttacc aaaatatcga caaaagatta
     4441 aatgaattgg gtgctaaaat tttgcgaata aattagctgc tgattttttc tatgaaaatt
     4501 tcaccaaaga taggatttct caactttcca aaggttttta tcattgcgat ggctcgtttg
     4561 tacttaattt tagcaactaa caaacctgat aaaaacatgt aatctatctg ggaggtattt
     4621 taagattctt ttttcctttc taaaatatca gtgagatttg cttatactaa aaaagctgtc
     4681 tggaaaaaag acagcttttt taaaacttat aaaaagaaaa tcaaaaagga agatcatcgt
     4741 ctatttcttc taattcaggt ggaatttgca cagactctac cgttgaagaa gtagcaattt
     4801 cttctgcggc ttcagaacgt ttgcccaata attgaatgtt ttctgcgtaa atttcagtta
     4861 tataacgttt tatgccatct ttttcccacg aacggctctg aattttacct tcaacataga
     4921 gttgactacc tttttttaca aatttttcta caatttctgc caatcctcgc caagctacaa
     4981 tattatgcca ttcagttctt tctggtactt gtgtaccatt ttgcatagta taacctcgct
     5041 cgcttgtagc aagaggaaat ttcgcaaccg ttacattctt atctaaatga cgtatttctg
     5101 ggtctttccc tacgttccct atcaaaataa ctttgtttac agacataact ttaaagttta
     5161 aggagttttt tattttgaaa taattttggt aaaaataaaa attcttttat gtttttcaaa
     5221 attaaatagg gaaaaaatta aaaaaacttt aatttttcat ttttggtatc ttaggagagc
     5281 ctttaaaaaa gcttatcttt catttttcag acaaacaaag gttttatttt tattccaaaa
     5341 atattatttt caaaacaact ggttattata caattcgatt aatcaattca attttaagtt
     5401 gattaatcaa tgaattagta gtattagcat ttaacaaggt caataaaaaa cccattttac
     5461 tcattctcca atcatgtaat atattaccgc tttttatatc agttaaataa atgtatttga
     5521 aatgttgctg cggtgataaa tctgctcttc gaatagtttg aatggcactc attactgcag
     5581 catcaatttc attaggcgat tcaattccta atgaatataa atctacggag gaatatatta
     5641 attcactatc ataaatagct tctaccaatg agtttaacat ttcatcttct aaaatagaaa
     5701 atttttttat tccattcatt tttgaaattt tttaatgaat tattgaatcg tatcttttat
     5761 ctgtttaaac atattttttt gagcaggggt caaattatca ggtatcttta tttgaagttg
     5821 aacaagcaaa tctccatatt catttgtttt tccgtaaata ggcatcccct tccctttgat
     5881 gcggagtatt ttattatttt gagtaccttc tggtatgtca atttttattt tcccatctaa
     5941 agtatcaaca atagtttttc ctcctaaaat agcatcaaac aaactaattt gatgaactat
     6001 tttaagatcg tcaccaattc tttcaaatac tggatgtggc aatattttta tttttaccaa
     6061 caaatctccg ttatttgcag gactacttcc tttcccacct ttacctttaa ttcttagaac
     6121 ttgtccatca taagcccctg gttttatttt gattctgatt ttttcattat ccagttgaat
     6181 aattctactt gttccatgat aagcttcttc aagagttaaa actatttcaa cttcataatc
     6241 ctgccctctt acttctgctt gtctaaaccc tctgccaaat ccacttgaag ctcctctacc
     6301 agcggctcct ctcccaaaaa atcgctcaaa gaagtctgaa aagccagaac cacgaccgcc
     6361 accaaataaa tcgttcaagt ctccttcata aaaaaactgt tgacctcctt gatgaccaaa
     6421 tccttcaaat ggacttcctc ctgaatagct accttcctgc gagaaacgat tccaatttgc
     6481 tcctaaatca tcatatttct ttcttttttc aggatcgccc aatacctcat aagcttcgtt
     6541 aataagctta aattgttcct ctgcttgctt attccctgga tttttatcag gatgatattt
     6601 tacagctaat tttcggtatg ctttttttat ttcttcttga gaagcatttt tatttactcc
     6661 caatatttta taataatctt tatattccat attcaatttt ttgaaaaaat tccttaatta
     6721 atataaagaa aaacatttcc catttccttt ttgttcgaga aaaaatgttt tttattgaat
     6781 ttcaatttgt ttaactgctt ttgttttggc ttcttctttg agaaatataa aaaaaatttc
     6841 cttttcatat tttgctccaa ttttatctgc cgccactgca gccagttaaa taaataaacg
     6901 aacaaaaaac taagaattta ttttacgata agtaaatcgt ttgttttctt ttaaacaccc
     6961 attttatttg atgaaataaa aagaacgcat tgtttacttc gatgttaaaa ttatctatct
     7021 tcgtttctgt tgcagccttc ttcaaacaaa acctttcatt gatgtcacta aatatttact
     7081 aaataaaaca aaatatttat ccataaaatg cggaaattaa atcacttaat atcaaatata
     7141 acttcaataa ataacttttt actgtcatat tttttattct cttttgcttt attttttgat
     7201 aaaaaatctc aaattaaata ccaactcagc ggtttttaaa taaaaacttc tttattacgt
     7261 cttcccaatt ttttatctac ctcaatatca attcatttac cacaatttct gtattcagta
     7321 ttaaattaat ttattctctc tctaatgaat gtgccaaaat gacattaaca atcaaaaaat
     7381 tgacataata gcaatctgtc tttgataatt catattagcc aaattctgca aagaatcaga
     7441 taacaaattt taattcttta ttcatctact aaaaattata gcaaaattat ttactttaat
     7501 ataccttctc ttatcaacaa ctgataccaa accgctatca aagtgtatcc ttaatccaat
     7561 gatataaaca gactgatt
//
LOCUS       DAOSAS010000088         7544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6313_length_7544_cov_12.930698, whole genome shotgun sequence.
ACCESSION   DAOSAS010000088 DAOSAS010000000
VERSION     DAOSAS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6313_length_7544_cov_12.930698"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLR72_05175"
     CDS             <1..203
                     /locus_tag="PLR72_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36625.1"
                     /translation="KNKKERMIVETESDTRQLIALKKRISELEQIIGHKQILIDFQSK
                     MIDLAEETYGVDIKIVKTVNVI"
     gene            complement(308..3703)
                     /gene="mfd"
                     /locus_tag="PLR72_05180"
     CDS             complement(308..3703)
                     /gene="mfd"
                     /locus_tag="PLR72_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOK36626.1"
                     /translation="MKGVNLILREFQDIYAQHPQIKALEKWTHSLEQNIKISGLSGSS
                     FALTLASLFKNVPQHFLIIQDDVDDAGYLYHDLKQLLSENEIFYYPSSYKQAIKLSQL
                     DAANEILRTEVLNKLIHDATPCVVVTYPDAIMQKVIAPQYIQKQTLKLKVGGRANLDE
                     IAETLFQYGFNKVDFVYEPGQFSVRGGILDIFSFSSEQPYRCDFFGNEIESIRIFDIE
                     TQLSKGIKNEIEIIPNLHHDNVSPHVSFFEFFPKNTIIVINNPSFIKERINQLYDEIL
                     VKINAQKSIADLHQTHITGNELINFITPFRKIETGTEKNYFKITSRLNFNTLPQPVFH
                     KNFDLISDHLKKKIEEGYQVYILSDSTKQTDRIAMIFEDHGDNIPFIPVKNTLHEGFI
                     DEDLKILCYTDHQIFDRFHKYQLKTDQARHGKVIMTLKELNQLKIGDYVVHVDHGIGT
                     FGGLVRMENNGKMQEMVKLIYKDNDILFVSIHSLHRISKYKGKEGETPRINKLGSGAW
                     ERLKEKTKSKVKNIARELIQLYAQRKAEQGFQFSLDSYLQQELEASFIYEDTPDQTKA
                     TADVKKDMETPIPMDRLICGDVGFGKTEIAIRAAFKAVADNKQVAVLVPTTVLALQHY
                     NTFKERLKDFPCNVEYLTRARSAKETKDILERLSEGKIDIIIGTHKLVGKSVKFKDLG
                     LLIIDEEQKFGVAVKEKLKQMKVNVDTLTMTATPIPRTLQFSLMGARDLSIINTPPPN
                     RYPVQTEIHQFDEDVIREAIQLEMNRNGQVFFVHNRIQNIYSVKSLIQRLVSGCRVAV
                     AHGQMPSDQLEEIIVDFINYDYDVLVTTTIIESGIDIPNVNTIIINNAHNFGLSDLHQ
                     LRGRVGRSNRKAFCYLIAPPFHLLTPEARHRLEAIETFTDLGSGFNIAMQDLDIRGAG
                     NMLGAEQSGFIAELGYDTYQKILNEAVQELKNEEFADLYKEEKEYTPTSFVADCQIDT
                     DLELLFPAEYVENVSERISLYRELDNIENENELLKFEKELEDRFGKIPPQGKNLIQLV
                     RLRWVAMYFGVEKLVLKNQQMIAYLVSNAESYYQSDIFGTLLQYAALHPTQCSFQEKN
                     GKRSIAFFHIKTVDDALHLFNELKANLEQIKFQPQEISSSFQK"
     gene            complement(3848..5398)
                     /locus_tag="PLR72_05185"
     CDS             complement(3848..5398)
                     /locus_tag="PLR72_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOK36627.1"
                     /translation="MLKKIRVVVSIVLFSLITLYFLDFRGFLPQSFSFLEKIQFIPAL
                     LAVNVAIIIILIALTLIFGRIYCSSVCPMGIFQDIIAWLSKKIIKRKRYTYSKAKTIL
                     RWSVLAAVVVTFLFGFTFLLGLLEPYGAYGRMATHLFRPIYMAGNNLLANILSSFGNY
                     SLYKVSIYSFGFFSTIIATITLLGIGFLAWRNGRTYCNTICPAGTLLGFLSNFSFLKI
                     QFKDERCNSCGLCVMKCKASCVDAKNKKIDYSRCVTCFNCIESCNRDAINYTFILQNT
                     KKNNPALTKDDNIKKIKSIDSSRRRFLSATLFSLVTGSTLLAQQTTSKLLPKKAFKRQ
                     TPVAPPGAISFEHFKKKCISCHLCVSKCPSHVIKPAFLEYGLSGVMQPHLYFDHGYCN
                     YNCTICGEVCPTGALKPLTVEEKHLTQMGQVQFIIENCIVYNDETSCGACSEVCPTQA
                     VSMIPYKDALTIPHIDTSICIGCGGCESACPAIPYKAIYVEGLKIQGKASIKHEQQEQ
                     IEIKDFGF"
     gene            complement(5419..6360)
                     /locus_tag="PLR72_05190"
     CDS             complement(5419..6360)
                     /locus_tag="PLR72_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOK36628.1"
                     /translation="MDRRDFLRAIALTGAALTTIKESKAMSILTQSFQTNNSTTKYDL
                     VAVMGGEPEEMFRKAIAEMGGIENFIKRGDKVCVKPNIGWDQPPEMGADTNPKLVAEI
                     IKQCLKAGAKEVIVFDHPCDEWRKCYTNSGIEAAAKAAGAKVVPANEESYYKPVSIPY
                     GKNLKSTKIHEAIVGSDKWINVPCLKNHGGAQLTISMKNYMGIVWDRGYFHAHDLQQC
                     IADVCTYEKRPVLNVVDAYRIMKNNGPRGKSPSDVVIAKGLFISQDIVAVDTAAANFF
                     NQVREMPIEKVKHILNAQALGLGTMNLDELKIKRIKL"
     gene            complement(6479..6877)
                     /locus_tag="PLR72_05195"
     CDS             complement(6479..6877)
                     /locus_tag="PLR72_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOK36629.1"
                     /translation="MTEKSWEEILQRFREIEFQETFDIIVAIANGGIIPAAILNQRLK
                     IEIQLLKINLRDAERKPIYDIPKLLSPINFNYVDKKILLVEDRIKTGATVQFAIELLK
                     DAAQIKTFAVNGKADYSLYNEPCFRFPWQL"
     gene            complement(6889..7398)
                     /locus_tag="PLR72_05200"
     CDS             complement(6889..7398)
                     /locus_tag="PLR72_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOK36630.1"
                     /translation="MENTIKLYSFNLSNLKTYLFAAIFVAGNLLLPQLVHLIPNGGLI
                     FLPIYFFTLIAAYKYGIHVGLLTAILSPLVNSLLFGMPPMNMLPIILIKSVILAIAAA
                     MVAKHFGKISFLGILLVILAYQIIGTGIEWAMTQNFFIAIQDFRIGLPGMVIQLVGGY
                     FVLKAIAKI"
BASE COUNT         2497 a         1320 c         1217 g         2510 t
ORIGIN      
        1 tgaagaacaa aaaggaacga atgatcgtag agactgagag cgatacacgt caattaatcg
       61 ctctaaagaa acgtatttct gagcttgaac aaatcatagg acataaacag attcttattg
      121 attttcagtc aaagatgata gatttggcag aagaaactta tggagttgat attaaaattg
      181 taaaaacggt caatgttatt taggcattaa taactcagaa ctaaaaactc gcattttcac
      241 ctcgagcctt atatccctat cccgcctcgt accatgtatc tcgaaactcg aaattcctaa
      301 actcttttta tttttgaaaa ctgcttgata tttcttgagg ttgaaatttt atttgctcca
      361 gatttgcttt cagttcatta aataaatgca aagcatcatc cactgtttta atatggaaga
      421 aagcaataga acgtttccca tttttttcct ggaaactgca ttgagtagga tgcaaagctg
      481 catattgtaa aagtgttcca aaaatatcgg attggtaata tgattcagca ttagaaacaa
      541 ggtaagcaat catttgctga ttttttaaaa cgagcttttc tacaccaaaa tacatggcca
      601 cccatctgag ccgaaccaat tggatgagat tttttccttg tggtggaatt tttccgaaac
      661 gatcttccaa ttctttttca aatttaagca attcattttc gttttcgata ttgtccaatt
      721 cacggtataa actgattcgt tctgaaacgt tttccacata ttcagctgga aataaaagtt
      781 ccagatcggt atcaatctga caatcagcaa caaatgatgt aggagtatat tctttttctt
      841 ctttgtataa atcagcaaat tcttcgtttt tcaattcttg gacagcttca tttaaaattt
      901 tttgataagt atcatatccc agttcagcaa taaaaccact ttgttcagca cctagcatgt
      961 ttcctgctcc tcgaatatcc agatcctgca tagcaatgtt gaatccgctg cctaaatcgg
     1021 taaaagtttc gatagcttcc aaccgatgtc gtgcttcagg cgtcaataaa tggaaaggtg
     1081 gagcaatcaa ataacaaaaa gcttttctgt tacttcttcc aactctgcca cgtaactgat
     1141 gcaaatcgct taatccaaaa ttatgagcat tattaatgat aatggtattg acattgggaa
     1201 tatcaatacc cgattcaata attgtagttg ttaccaatac atcataatcg taattaataa
     1261 aatcaacgat gatttcttcc agttggtcgg atggcatttg tccatgagca actgctactc
     1321 tgcatccaga cacgagtcgt tgaatcaatg atttgacaga ataaatattt tgaatgcggt
     1381 tatgaacaaa aaatacttgt ccatttctat tcatttcaag ttgaatggct tcacgaatca
     1441 catcttcatc gaattgatga atttctgttt gtactggata acggttgggt gggggagtat
     1501 tgataatgga taaatctctg gctcccatta aggaaaattg caaagtacgt ggaataggcg
     1561 tagcagtcat ggtcaacgta tcaacattta ctttcatctg tttcaatttt tccttaactg
     1621 caacaccgaa tttttgctct tcatcaatga taagcaatcc caaatctttg aattttacag
     1681 atttaccaac taatttatgc gttccaataa taatatcaat ttttccttca cttaatcgtt
     1741 ctagaatatc tttggtttct tttgcacttc gggcacgtgt taaatattcc acattgcaag
     1801 gaaaatcttt taatctttct ttaaatgtgt tgtaatgctg caaagccagc acagttgtag
     1861 gtaccagtac agcaacctgc ttattatcag ccacagcttt aaaagcagca cgaatggcta
     1921 tttctgtttt tccaaaacct acatcaccac aaattaaccg atccattgga atgggtgttt
     1981 ccatgtcttt ttttacatca gcagtagctt ttgtctgatc aggtgtgtct tcataaataa
     2041 acgaagcttc cagttcctgt tgtaaatagc tgtcaagtga aaattgaaaa ccttgttctg
     2101 cttttcgttg tgcataaagt tgaatcaatt cacgagcaat attctttact ttcgattttg
     2161 tcttttcctt cagtcgttcc caggctcctg aaccaagttt attaatacga ggagtttcac
     2221 cttctttacc tttgtatttt gaaatgcggt gaagtgaatg aatactcaca aaaagaatgt
     2281 cattgtcttt ataaatgagt ttcaccattt cctgcatttt tccattgttt tccatgcgaa
     2341 ccaagcctcc aaaagtgcca atcccgtgat ccacatgcac tacgtaatcg ccaattttca
     2401 attgattcaa ttctttgagc gtcataatga ctttcccatg acgagcctgg tcggttttca
     2461 actggtattt atgaaaacgg tcaaaaatct gatgatcagt gtaacataaa atttttaaat
     2521 cttcgtcgat aaaaccttca tgcaaagtat ttttgacagg tataaatgga atattatcgc
     2581 catgatcttc aaaaatcatg gcgattcggt cagtttgttt ggtgctatca ctgaggatat
     2641 aaacttgata accttcttca atttttttct ttaaatgatc gctgattaaa tcgaaatttt
     2701 tgtgaaaaac aggttgtgga agagtattaa aattcaatcg agaagttatt ttaaaataat
     2761 ttttctctgt tcctgtttct atttttcgaa atggcgtgat aaagtttatt aattcattcc
     2821 ctgtaatgtg cgtttgatgt aaatcagcaa tacttttctg ggcatttatt ttaacgagaa
     2881 tttcatcata caattgatta attcgttctt taataaaaga aggattgtta atgacaataa
     2941 tggtattttt ggggaaaaat tcaaaaaatg aaacatgagg agaaacgttg tcgtgatgca
     3001 aatttggaat aatttctatc tcgtttttta ttccttttga aagttgggtt tcaatatcga
     3061 atatacgaat ggattcaatt tcgttaccaa aaaaatcgca acgataaggt tgttcagaag
     3121 aaaaagaaaa aatgtccagt attccaccgc gaacagaaaa ttgacccggt tcatagacaa
     3181 aatctacttt attgaagccg tactggaata gtgtttctgc aatttcatcc agattagctc
     3241 gtccaccaac ttttaattta agtgtttgtt tttgaatgta ttgtggggca attacttttt
     3301 gcataatggc atcaggataa gttaccacca cgcaaggagt tgcatcatga atgagcttgt
     3361 ttaatacttc tgttcttaaa atctcatttg ctgcatctaa ttgagataat ttaatagctt
     3421 gtttataaga agaaggatag taaaatatct cattttcaga aagtaattgt ttcaggtcat
     3481 gataaagata gccagcatca tccacatcgt cctgaataat gagaaagtgt tgaggaacat
     3541 ttttaaaaag ggaagctaaa gttagagcaa aagaagaacc gctcaaacca gaaattttta
     3601 tattctgttc gagggaatga gtccactttt caagtgcttt aatttgtgga tgctgtgcgt
     3661 aaatgtcttg aaattctcta agaatcaaat taactccttt catcattaca taaacattta
     3721 acaaccaaat atttatgtaa tagaaaattg ttctaaaatt tcactgcaaa ggtagtaatt
     3781 ttaaaggaaa gtggaaaatt aaaaggatga gaatttttaa agaaaacagc tctgaaagga
     3841 aaaaaattca aaatccgaaa tcttttattt ctatttgttc ttgttgttca tgttttatgc
     3901 tggctttacc ctgaattttt aatccttcta cataaatagc tttgtaagga attgctggac
     3961 aagctgattc gcaaccgccg caacctatac aaatggaagt gtctatatga ggaatggtaa
     4021 gtgcatcttt ataaggaatc atgctaactg cctgcgtggg acaaacttct gaacaagcac
     4081 cacaactggt ttcgtcatta tagacaatac aattttcaat aatgaattgt acttgaccca
     4141 tttgcgtaag atgtttttct tctactgtta atggtttgag tgcacctgta ggacaaactt
     4201 caccacaaat ggtgcaatta taattgcagt aaccgtggtc aaaatataag tgaggttgca
     4261 ttactccact taaaccatat tccagaaaag ctggcttaat cacgtgagaa ggacatttgc
     4321 tcacacacag atgacaggat atacattttt ttttaaaatg ttcaaaacta atggcacctg
     4381 gaggagcaac aggagtttga cgcttgaatg ctttttttgg aagtaattta ctggtagttt
     4441 gttgagccag caatgtgctt cctgtcacaa gagaaaacaa tgtggctgaa agaaaacgtc
     4501 gtctcgaaga atcaatggat tttatttttt ttatgttatc atctttagta agagctggat
     4561 tgttcttttt agtattttgc aaaataaagg tataatttat tgcatctcga ttacaacttt
     4621 cgatacaatt gaaacacgta acacaacgac tataatcaat ttttttattc tttgcatcaa
     4681 cacatgaagc tttgcacttc ataacacaca aaccacatga attacatctc tcatctttaa
     4741 attgaatttt taaaaaagaa aaattgctca aaaaaccaag aagcgttcct gcaggacaaa
     4801 tagtattaca ataagttcta ccatttcgcc aggctaaaaa accaatcccc aacaaagtaa
     4861 tggtagcaat aatagttgaa aaaaatccaa aactataaat agaaacttta tataaactat
     4921 aattaccaaa tgaactcaaa atattagcaa gtaaattatt cccagccata taaataggac
     4981 ggaaaagatg agtggccatt cttccataag caccataagg ctcaagcaaa cccagcaaaa
     5041 aagtaaatcc aaaaaggaaa gtaacaacaa ctgcagccaa cacactccag cgaagaatag
     5101 tttttgcttt gctataagtg taacgttttc tttttatgat ttttttggat aaccaggcta
     5161 tgatatcttg aaaaattccc attggacaaa ctgaagaaca atatattcgt ccaaatatta
     5221 atgtgagcgc tatcaaaatt ataataatag ccacatttac tgctaataag gcaggaataa
     5281 attgaatttt ttctaaaaaa ctgaaacttt gtggcaaaaa acctctgaaa tccagaaaat
     5341 aaagagtaat taatgaaaac aaaacaatgg aaacaaccac tcgaattttt ttcagcatac
     5401 gtttttattc aggaaaaatt acaacttaat tcttttgatt tttaattcgt caagattcat
     5461 tgtccctaat cccaaagctt gggcattaag aatatgtttt actttttcaa tgggcatttc
     5521 acgtacctga ttaaagaagt tagccgctgc cgtatccact gcaacgatgt cttgtgagat
     5581 aaaaagtcct tttgcaatga ctacatcgga aggagatttt cctctcggtc cattattttt
     5641 cataattctg tatgcatcaa ctacgttcaa aactggacgt ttttcgtaag tacaaacatc
     5701 ggcaatacat tgctgtaagt cgtgagcatg gaaataacct ctgtcccaaa caatgcccat
     5761 ataatttttc atagaaatag tgagttgtgc gccgccgtga tttttcaggc aaggaacatt
     5821 aatccattta tcactgccta caatagcttc atgaattttt gtcgacttca aatttttacc
     5881 ataaggaatg gaaacaggtt tataataaga ttcttcattt gctggaacaa cttttgcgcc
     5941 cgcagctttt gcagctgctt caataccact attggtataa cattttcgcc attcatcgca
     6001 aggatgatca aaaacaatca cttctttggc accagctttt agacattgtt ttatgatttc
     6061 agctaccagt tttggattcg tatcagctcc catttctggt ggttgatccc agccaatatt
     6121 cggtttaaca cagactttgt caccacgttt tatgaagttt tctattccgc ccatttcggc
     6181 aatagctttg cgaaacattt cttcaggttc acctcccatg acagctacca aatcgtattt
     6241 tgtggtggaa ttatttgttt gaaacgattg ggtaagaata ctcatcgctt tactttcttt
     6301 tatagtcgtt aatgctgctc ctgttaaggc aattgctcgt aaaaaatcac gcctatccat
     6361 aatttgtttt attttgaaat aattttagtt acaagttaaa ttaagatatt aattgattga
     6421 ttattaattt tttaatgaat caagaaaaac attaaaaatc tattatgaaa tgttcagttt
     6481 atagttgcca cggaaatcga aagcagggtt cattataaag tgaataatca gctttaccat
     6541 ttactgcaaa agttttgatt tgagcagcat cttttaaaag ttcgatggcg aattgtacag
     6601 ttgcccctgt tttaatacga tcttccacca gaagaatttt tttatctaca taattaaaat
     6661 tgatcggaga caaaagtttt ggaatatcat atattggttt ccgctctgca tctcgtagat
     6721 ttatttttaa aagttgaatt tcaatcttca atcgttggtt gagaatagca gcaggtatga
     6781 tgccaccatt cgcaatggca acaattatat caaaagtttc ctgaaattca atttcacgaa
     6841 aacgttgcaa aatttcttcc catgattttt ctgtcatcgg actcaatttt atattttagc
     6901 tatggctttc aaaacaaaat aaccacctac aagctgaatc accattcctg gtaaacctat
     6961 tcgaaaatct tgaatagcga tgaaaaaatt ttgtgtcata gcccattcta tgcctgtgcc
     7021 tataatttga taagcaagga ttaccaacaa aattcctaaa aaagatattt ttccaaaatg
     7081 cttagcaacc attgcagcag caatagccag aataacagat ttaattaaaa taattggcag
     7141 catgttcata ggtggcatac caaacaaaag gctattcaca agtggagata aaatagctgt
     7201 taacaatcca acgtgaatac catatttata agcagcaatc agcgtaaaga aataaatagg
     7261 cagaaatata agtccaccat ttggaataag atgcaccaat tgtggtaaga gtaaatttcc
     7321 agcaacaaaa atcgctgcaa aaaggtaggt ttttaaattg ctcaggttaa aagaatacag
     7381 ttttatagta ttttccatgt tttaaaattt gaagttgatt aaacatcttt actacaaaac
     7441 tataaataat taattgaata tttccttaca tacgcttatt atttttattt aaaacatata
     7501 aaattcatta aggtttttta aatttttaag attaaaaaac taat
//
LOCUS       DAOSAS010000089         7420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6429_length_7420_cov_12.535913, whole genome shotgun sequence.
ACCESSION   DAOSAS010000089 DAOSAS010000000
VERSION     DAOSAS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6429_length_7420_cov_12.535913"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..922
                     /locus_tag="PLR72_05205"
     CDS             86..922
                     /locus_tag="PLR72_05205"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOK36631.1"
                     /translation="MVLGNIVSYFSNDTLILSQNSISRIEDSVSKINLADTDLLSRRL
                     STKIDTIHAINHLEGFEGILHPSSPQTENWVIVLLIVLLSILLVFLYQHPSFITENIQ
                     NFFKIKIDKYRTNIGTSYSFFYSFIFLFSIGVFSLYAYILFYNPAEGFLFSRYFYFCG
                     ITLAFFILKYVFIQLIGFVFFEPNIFTVLKANYFNTFYFFNIILFFLVILQIYFPVYD
                     IKWTLYFALILGILALTSITTKLLKIFSHKKFVYFYILLYLCTLEFLPLIALFRAYQL
                     MY"
     gene            949..1725
                     /locus_tag="PLR72_05210"
     CDS             949..1725
                     /locus_tag="PLR72_05210"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOK36632.1"
                     /translation="MKIKKILISQPQPNSEKSPYFAISEKYNVKIDFRPLIKVEPLSA
                     KDFRAQHISILDHTAIIFNARHGIDHFFRLCEEMRITVPETMKYFCVSETVALYLQRY
                     IQYRKRKVFFSPTGKLSDLVTLMKKHPDEKYLFVTSDVPNEENMSILVNSNLNFDKAI
                     MYRTVSNDFDPEEEFNYDMIIFFSPVGVSSLLKNFPDFVQGDIQIGCFGASTAQAVRD
                     AGLRLDLEAPQPGLPSMTMALDNFLKEFSKKENSNKKATK"
     gene            1809..2213
                     /locus_tag="PLR72_05215"
     CDS             1809..2213
                     /locus_tag="PLR72_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOK36633.1"
                     /translation="MKNRQFSFPKSERLHSKIKINRLFTDGKSFIVYPLKIFYNFEPS
                     EEFNVCILISVPKKQFKKAIHRNRIKRLLRETYRLNKHLIFEKFSQRKFQIQLAFVYV
                     ANEEIDFYKLNEKMKIALQKLIELVDHDKESY"
     gene            2197..2418
                     /gene="yidD"
                     /locus_tag="PLR72_05220"
     CDS             2197..2418
                     /gene="yidD"
                     /locus_tag="PLR72_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOK36634.1"
                     /translation="MKKAIEFIVLLPVYIYRYTISPLFPPSCRYTPTCSQYAIEAVKK
                     HGIFKGGWLAVKRLSRCHPWGESGYDPVP"
     gene            2514..3602
                     /gene="lpxK"
                     /locus_tag="PLR72_05225"
     CDS             2514..3602
                     /gene="lpxK"
                     /locus_tag="PLR72_05225"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOK36635.1"
                     /translation="MKNQFIHIILYPFAVFYGLAVGIRNWLYDRGIFHSTAFSIPIIG
                     VGNLSVGGTGKTPHTEFILSYVQDEWKTAFLSRGYKRKTKGFFLADEKSTYQTIGDEP
                     YQIYRKFPKVIVAVDEKRVDGVMKLQKQFPDLQLVVMDDAFQHRQIKIGLTILLTDYF
                     HLYTRDRLLPTGTLREQKSGSKRADIVIVTKCPPDLKPIDMRLVEKELNLTGRQQLFF
                     STYEYEEIVPVFPEKEEKKYSLQQINEKNGSLLIVTGIASPEKMIEYLRQFSNLIESL
                     IFPDHHDFSKKDIEKMEQKFAQLPANEKIVIVTEKDAVRLLNNAFLSEFLKSKIYYLP
                     IKVKILNHQEDSFIQKIKNYVRKNSKSN"
     gene            3574..4386
                     /locus_tag="PLR72_05230"
     CDS             3574..4386
                     /locus_tag="PLR72_05230"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOK36636.1"
                     /translation="MLEKIQKATDFLSAKITTSPKTGIILGTGLGNLITQMDISLEIP
                     YETIPYFPVSTVEGHQGKLIVGKLGGVEVIAMQGRFHYYEGYDMKQVTFPVRVMYALG
                     IRNLLVSNAAGGMNPDFEIGDIMIITDHINLFPEHPLRGKNYDELGPRFPDMSEAYSK
                     DLIAKAKQIAVRNNIKVVEGVYIGTSGPTFETPAEYRYFRTIGGDAVGMSTVPEIIVA
                     NHAGMKCFGISIITDLGVPGKIVEVSHEEVQRIGNSVQPLMTLIMKELVKEI"
     gene            complement(4454..6409)
                     /locus_tag="PLR72_05235"
     CDS             complement(4454..6409)
                     /locus_tag="PLR72_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOK36637.1"
                     /translation="MMKNFFVKLLSVFIFCIISVLSLYAVKAYPYPIIVKQPDGSEVT
                     IRIHGNEFQHLKTTLDGYPVKQNAKGFWMYATIDNAGKLKESKYVARNAENRSATERQ
                     FLNSLQTKKNVPQRIKSVNPILEKIKQPVLRKAFPLTNSPKSLVILVNFSDRSFLTPD
                     AQTAFHNMLNQSDYSTNGATGSARDYFMSSSYGNFSPIFDVVGPFDLDKDMSYYGAND
                     DSGYDVNPAALIVDACAAADAAGVDFTQYDTDNDGEIDNIFVYYAGYNEAEGGDENTI
                     WPHRWVVYTSKENPIYYTYDGTIESVTFDGKRLYDYACSSELKGNLGTNMAEIGTFCH
                     EFGHVLGLPDYYHTEEDKETLNGWDIMDAGSYNNNSRTPPTYSTYNRFYLGWLTPQEV
                     NTPSNLILLPIYQEKTKPAHTLQQSYLLSATSHNLNGNDPLPREFFMLEYRKKIGWDA
                     YLPAEGMLIWHIDYDPTAWDENTVNNYSGTSQTADSHMRVYLQPLSGSTTTPGTAFTS
                     GSFTPITWTGVNINRPITQITKSDDKITFKLMGGAGEPVIEIGKVDQQLKFARIPVNT
                     QSKKFLNIKTTDLTGDLTLSISGEQASYFLISTSSISKEAANSAEGTNITITYQPTSA
                     GIHTATFTISGGGLDPAKIITLTGEAY"
     gene            complement(6577..7305)
                     /locus_tag="PLR72_05240"
     CDS             complement(6577..7305)
                     /locus_tag="PLR72_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK36638.1"
                     /translation="MKIILDHNSPIPLYAQIEDQIREAIKSPEYKNGKMLPSEVDLSK
                     QLGISRSTLRHAINKLVFEGLLVRKKGIGTFVANKAVSTKLSNWLSFTQEMRALGIEV
                     KNYELHVQWEKPNEELCNFFNISPNTKILRLERLRGKPEYPFVLFISYFNPRIGLTGN
                     EDFSRQLYDILENDYNTIVKYSKEEISAKIADKKLAEKLQINVGDPILVRKRHVYDPG
                     NHPVEWNIGYYRADSFVYTIEAER"
BASE COUNT         2474 a         1139 c         1171 g         2636 t
ORIGIN      
        1 taattttatc ataggagtaa agcattctaa tgataatgaa agtgaatgaa gaagtaattt
       61 atttagtgcg aaattaaatg tttttatggt tttaggaaat attgtttctt atttttctaa
      121 tgatacgttg attttgtctc aaaattctat tagtagaata gaagactctg tatcaaaaat
      181 taacctagca gatactgatt tattatccag aaggctttcg acaaaaattg atacgattca
      241 tgcgattaat catttggaag gttttgaagg tattttgcat ccttcctctc cgcaaactga
      301 aaattgggtg atagttttat tgattgttct tttaagtata ttattggttt ttttatacca
      361 gcatccttcc tttattactg aaaacattca aaattttttt aagattaaaa ttgataaata
      421 tcgaaccaat attggaacca gttattcttt tttttattct tttatttttc ttttttctat
      481 tggcgtattt agtttatatg cgtatattct cttttacaac cccgcagaag gttttttgtt
      541 tagtcgctat ttttattttt gtggcatcac gttggcattt tttatcctca aatatgtttt
      601 tattcaatta attggtttcg tcttttttga acctaacatt tttacagtat taaaagctaa
      661 ctattttaac acattttatt tttttaatat cattttattt tttctggtta ttttacaaat
      721 ttattttcct gtgtatgata taaaatggac tttatatttt gcattaatat tgggtatatt
      781 agctttaaca agcattacaa ccaagttatt aaaaatattt tctcacaaaa aattcgttta
      841 tttctatatt ttgttgtatc tttgcacact tgaattttta ccattaattg cgctctttag
      901 agcttatcaa ttaatgtatt aagtgtttta tttttaaaca ataaagcctt gaaaataaaa
      961 aagatactta tttctcagcc gcagcctaat agtgaaaaat caccttattt tgctattagt
     1021 gaaaaataca atgtaaaaat agattttaga cctcttatca aagttgagcc tctttctgca
     1081 aaagatttta gagcccaaca tatttctatt ctggatcata ctgccattat ttttaatgca
     1141 aggcatggaa ttgatcattt tttcagatta tgtgaagaaa tgcgcattac ggtacctgaa
     1201 acaatgaaat atttctgtgt ttcagaaaca gttgctttgt atttacaacg ctatatccaa
     1261 tatcggaaaa gaaaggtctt ttttagtccg acaggtaagt tgagtgattt agtgaccctc
     1321 atgaaaaagc accctgacga aaagtattta tttgttactt ctgatgtgcc gaacgaagaa
     1381 aacatgtcga tattggtcaa ttcaaattta aatttcgata aagcgataat gtatcgaaca
     1441 gtgagcaatg attttgatcc cgaagaagaa tttaattatg atatgattat ttttttcagc
     1501 cctgtgggag taagttcatt gttaaagaat ttccctgatt ttgttcaagg tgatattcag
     1561 attggatgtt ttggagcaag tacagctcaa gccgttcgtg atgctggctt acgtttagat
     1621 cttgaggcac ctcagccagg tttaccttcc atgacaatgg cactggataa ttttctgaaa
     1681 gaattttcaa aaaaagaaaa tagcaataaa aaggctacaa aataaaaaag caataagtta
     1741 gtggatgtta atattatccc aaaagtttag atatttttct tacttttggg ataatttatt
     1801 ttattgaagt gaaaaatcga caattttcat tccctaaatc agaacgattg catagtaaaa
     1861 ttaaaattaa tcgtttattt actgacggta aaagttttat tgtttatcct ttgaaaattt
     1921 tttataattt tgaaccgtca gaagaattta atgtttgtat attaataagt gttcccaaaa
     1981 aacaatttaa aaaagctatt catcgaaata gaataaaacg cttattaaga gaaacttaca
     2041 ggttgaataa acatttgatt tttgaaaagt tttctcaaag aaaatttcag attcaactgg
     2101 catttgttta tgttgctaat gaagaaatag atttttacaa actgaatgaa aaaatgaaaa
     2161 ttgcactaca gaaattaatt gaattggttg atcatgataa agaaagctat tgaatttatt
     2221 gttttattgc ctgtgtatat ttatcgatac actatttcac cacttttccc acccagttgc
     2281 agatatacac caacttgctc gcaatatgct attgaagctg taaaaaaaca tgggattttt
     2341 aagggaggtt ggttagctgt aaaacgactt agccgctgtc atccgtgggg tgaaagcggt
     2401 tatgatccag ttccttaaca tttaattttt caaaagacaa aaagttcttt tatttgaaaa
     2461 aagaatcatt ttatttttac tccttttgaa ttttttccaa caaaacaaac tttttgaaaa
     2521 atcaatttat ccatattatt ctttatccat ttgctgtttt ttatggctta gctgttggaa
     2581 tacgtaactg gctttacgat agaggaattt ttcattctac agctttttca attcctatta
     2641 taggggttgg aaatctttcg gtaggcggaa caggaaaaac tccgcatact gaattcattc
     2701 tttcttatgt gcaagatgaa tggaaaacag cttttttaag tagaggctac aaacgaaaaa
     2761 caaaaggatt ttttctggca gatgaaaaat caacgtatca aaccattggg gatgaacctt
     2821 atcaaattta tcgcaagttt ccaaaagtca ttgtggctgt ggatgaaaaa agggtagacg
     2881 gtgtaatgaa acttcaaaaa caatttcctg atcttcaact ggtcgtaatg gatgatgcct
     2941 ttcaacacag gcaaattaaa atcgggttaa ccatcttgtt gacggattat tttcatcttt
     3001 atacacgcga taggcttttg ccaacaggaa cacttcgtga acagaaaagt ggaagtaaaa
     3061 gagcagatat agtaatagtt accaaatgtc ctcccgattt aaagcctatt gatatgcgtt
     3121 tagttgaaaa agaattgaat ttaactggta gacagcaatt gtttttttca acttatgaat
     3181 atgaagaaat tgttcctgta tttcctgaaa aagaagaaaa aaaatattca ctccagcaaa
     3241 taaatgaaaa aaatggcagt ttattaatag tgacaggaat tgcttcacct gaaaagatga
     3301 tagaatattt aagacaattt tcaaatttaa tagaaagctt gattttccct gatcatcatg
     3361 atttttcaaa aaaagatatt gaaaaaatgg agcaaaaatt tgctcaactt cctgcaaatg
     3421 agaaaattgt tatcgttaca gaaaaagatg ccgttcgttt attaaataat gcttttttat
     3481 ctgaattttt aaaatcaaaa atatattatt taccgattaa ggttaaaatt ttaaatcacc
     3541 aagaagattc atttattcaa aagattaaaa actatgttag aaaaaattca aaaagcaact
     3601 gatttcctat cggctaaaat tacaacttct ccaaaaacag ggattatttt aggtacaggt
     3661 ttaggaaatt taattactca aatggatatt tctttggaaa taccttatga aaccattcct
     3721 tattttcctg tttcaacagt agaaggacat caaggcaagt tgattgttgg gaagttaggt
     3781 ggtgtggaag taattgccat gcaagggcgt tttcattatt atgaaggtta tgatatgaaa
     3841 caagtaactt tccctgtccg ggtgatgtat gctttaggta ttcgtaattt attggtatcc
     3901 aatgcagcag gtggtatgaa tcctgatttt gagatcggtg atataatgat tataaccgat
     3961 catatcaatc tttttcctga acatcctttg cgaggtaaaa attatgatga attaggaccg
     4021 cgtttccccg acatgagcga agcttattca aaagatttga ttgccaaagc caaacaaatt
     4081 gcagttagga ataatattaa agtagtagaa ggtgtttaca ttggaaccag tggtcctact
     4141 tttgaaacgc cagctgaata tcgttatttt cgtactattg gcggcgatgc tgtaggcatg
     4201 agcactgttc ctgaaataat cgtagccaat catgctggta tgaaatgttt tggtatttcc
     4261 attattaccg atctgggcgt tcctggcaaa attgtagaag tttcacatga agaagtacaa
     4321 cgaattggca actcagttca accacttatg acattaatca tgaaagagct ggtaaaggaa
     4381 atataatttc atttgttcaa ttaaaacaaa aaaggaacga tacccaaaaa attatcgttc
     4441 cttttatttt ttatcaataa gcttcaccag tcaaggtaat aatttttgca ggatccaatc
     4501 cgccgcctga gatggtaaag gttgctgtat gtattcctgc tgaagttggt tgataagtaa
     4561 tggttatgtt agtaccttca gcagaatttg cagcttcttt tgaaatactt gaagtagaaa
     4621 tcaaaaaata acttgcttgt tcacctgaaa tagaaagtgt taaatcgcct gttaaatcag
     4681 tagttttaat atttaaaaat ttcttgcttt gtgtattgac tggaatacga gcaaatttta
     4741 attgttgatc aacttttcct atttcgataa caggttcacc agcaccaccc attaatttaa
     4801 aagttatttt atcatcactt tttgtgattt gtgtgatagg cctattaata ttcacacctg
     4861 tccaagtaat tggagtaaat gaaccgctcg taaaagctgt gccaggtgtt gttgtagaac
     4921 cacttaatgg ttgcaaataa acacgcatgt ggctatcggc agtttgtgaa gttcctgaat
     4981 aattgttaac ggtattttcg tcccatgcag ttggatcgta atcaatatgc cagattaaca
     5041 ttccttcagc aggaagataa gcatcccagc ctattttttt acggtattct aacataaaaa
     5101 attcgcgagg taatggatcg ttaccattca aattatgact tgtagccgac aacaaatagg
     5161 attgttgtaa agtatgagca ggttttgttt tttcttgata aatgggtaaa agaataagat
     5221 tactaggcgt atttacttcc tgtggcgtca accagcctaa ataaaaacgg ttgtatgttg
     5281 aataagttgg aggagttcta ctattattat tataagaccc agcatccatt atatcccaac
     5341 catttaaagt ttctttatct tcttctgtat gataataatc aggtaaacct aacacatgcc
     5401 caaattcgtg acaaaaagtt ccaatctcag ccatatttgt acctaaatta cccttcaact
     5461 cagagctaca agcataatca tataatcttt taccatcgaa agttactgat tcaatcgtgc
     5521 cgtcataagt gtaataaatt ggattttctt tacttgtata aactacccat ctgtgaggcc
     5581 aaatcgtatt ttcatcgcca ccttcagctt cgttatatcc tgcataataa acaaaaatat
     5641 tgtctatttc tccatcgtta tctgtatcat actgagtaaa atccacacca gcagcatcag
     5701 cagctgcaca agcatctaca attaaagcag ccggatttac atcataacca ctatcatcat
     5761 tggcaccata ataactcata tctttatcca agtcaaaagg acctacaaca tcaaatattg
     5821 gagaaaaatt tccataagaa ctcgacataa aataatcacg tgctgaacca gtagcaccat
     5881 tagtactata gtcgctttga tttaacatgt tatggaaagc agtttgagca tcaggggtta
     5941 aaaaacttct atcactaaaa tttaccaaaa taaccaatga tttaggagaa ttggttaacg
     6001 ggaaagcttt ccgtaaaaca ggttgcttta ttttttctaa tattggattt acagatttaa
     6061 ttcgttgagg aacatttttt ttagtttgta aagaatttaa aaattgtctt tcagttgctg
     6121 aacgattttc ggcatttcga gctacatatt tgctttcctt taatttacct gcattgtcaa
     6181 ttgttgcata catccaaaat ccttttgcat tttgctttac cggataacca tcaagagtag
     6241 tttttaaatg ttgaaactca ttgccatgaa ttcgaatagt aacttcactg ccatcaggct
     6301 gttttactat gataggataa ggataagctt ttactgcata gagagatagt accgagatta
     6361 tgcagaaaat aaataccgat aatagcttta caaaaaaatt tttcatcata taagaagagg
     6421 atttaagtaa ctaattaaat aaaaaatatt ttgctggaat atttttataa tttatacact
     6481 catctctttc taaaattaaa aaattaatat tcaacgactt acgaatcaag agatcctaaa
     6541 attgtttttc ggaaaggatt taacttaaat ttattattac ctttcagcct ctattgtata
     6601 aacgaaacta tccgctctat aatatccaat attccattcc acaggatgat ttccgggatc
     6661 gtaaacatgt cgtttacgta caaggatagg atcacctaca ttgatttgta gcttttctgc
     6721 taatttttta tcagcaattt ttgcacttat ttcttctttt gaatacttaa caatcgtatt
     6781 gtaatcattt tctaaaatat catacagttg acgagaaaaa tcttcattac cagttaaacc
     6841 aattcgtgga ttaaaataag aaataaaaag tacaaaagga tattctggtt ttcctcttaa
     6901 tcgttccaat ctcaaaattt tagtattagg agagatatta aaaaaattgc ataattcttc
     6961 attaggtttt tcccactgaa catggagctc ataattttta acttctatac ctaatgctcg
     7021 catttcttgt gtaaaactta gccagttact taatttagtg cttacagctt tatttgccac
     7081 aaaagtacct attccttttt tcctcaccaa caaaccttca aaaactagtt tattaatagc
     7141 atgtctaagg gtacttcgtg aaatgcctaa ttgtttagac aaatcaactt cacttgggag
     7201 catttttcca tttttatatt cgggtgattt aatagcttcc cgaatttggt cttcaatttg
     7261 agcatataaa ggtatagggc tattatgatc caatattatt ttcatagtca atgaattaaa
     7321 ttgcaaattt aattattatt tttaatttaa ataaattttt ttgttaaaag ctttacttta
     7381 agagtagctt aaattttaag aatatgtttg atttttaatc
//
LOCUS       DAOSAS010000090         7197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6664_length_7197_cov_15.184262, whole genome shotgun sequence.
ACCESSION   DAOSAS010000090 DAOSAS010000000
VERSION     DAOSAS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6664_length_7197_cov_15.184262"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..465
                     /locus_tag="PLR72_05245"
     CDS             289..465
                     /locus_tag="PLR72_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36639.1"
                     /translation="MQKKSTSKKKTKLSKSSFTVGGFELHPQKKTIKTILQFASSYRT
                     QKVNNTNFIALNLN"
     gene            709..>1169
                     /locus_tag="PLR72_05250"
     CDS             709..>1169
                     /locus_tag="PLR72_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36640.1"
                     /translation="MEEKKLTIREWAEEDRPREKMIRKGIQSLSDAELLAILIGSGNK
                     NESAVELSRRILHECKDNLNELAQLSIDDFCKRFKGIGTAKAVTIMAALELGKRRKTT
                     EIIEREKITSSHDLCKLFEPMLIDLPHEEFWVAILNTANKVMEVFRLTQGGT"
     assembly_gap    1170..1269
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1320..2228
                     /locus_tag="PLR72_05255"
     CDS             1320..2228
                     /locus_tag="PLR72_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK36641.1"
                     /translation="MAFLEIENIVKKYEHQVALNGVSLNVPENSIYGLLGPNGAGKTT
                     LIRIINRMIAPDSGEVRLNGKIIKDDDLVLLGYMPEERGLYKKMKVGEQALFFAQLKG
                     MKKGDALKALKYWFEKFEIQSWWNRKVEELSKGMAQKVQFIITVLHQPKLLILDEPFS
                     GFDPINTDIIKQEILQLKNNGTTVIFSTHNMSSVEELCENISLIHQAKIILQGKVSDI
                     KASYSNRTFKIRSLQPISENEHFKLITQIQKDGMIESIIKKKTNESNNVLLSELIQQT
                     EIVAFEELLPSMNDIFIETVQKEELN"
     gene            2279..3616
                     /locus_tag="PLR72_05260"
     CDS             2279..3616
                     /locus_tag="PLR72_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK36642.1"
                     /translation="MPNKIQLIIRREYTTRVMKRSFLLLTFLTPLLFAALIIVPLWLS
                     TLKTETKKHILVIDRTHQYEKALKNNDSYIFEFVDKPLNEIQKNTNKNKELAGVLVIT
                     GDLSENSNAVTLYSEKQINLETKNYIAELLNDYVKEQKLRNYNVPDLEKIIENTNQKI
                     DIATVRWNEKGEEKLSSTELTIAIGIFSAMMVYIFILTYGSQVMSGVIQEKTNRIVEI
                     IISSVKPFQLMMGKIIGIALVGLTQFFIWMILTLVIVGIAGSLFNTNIDFNSLTQSNE
                     IIKNEQIVNQAVSSSEAENAMNHFLEMLQGVDFMQIITLFIVYFIGGYLLYASLFAAV
                     GSAVDSETDTQQFTLPLTLPIIFAIYAAIYSTQNPDGPLAFWCSLIPFTSPVVMMVRL
                     PFGVATWEILLSLVILILSFIGTTWLAGKIYRTGILMYGKKATWKELWKWMKY"
     gene            3697..4602
                     /locus_tag="PLR72_05265"
     CDS             3697..4602
                     /locus_tag="PLR72_05265"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOK36643.1"
                     /translation="MIPPFLKSGDRLQIISPASCIDQSYIEGAKKVLSSWGLEVAEGK
                     FARNVYGRFAGTKEQRINDLQKALDDPTLKAILCSRGGYGLAQIIDKIDFSNFIKFPK
                     WLIGFSDISILHAAIQQYGIVSIHGIMAKQLTELDPLSEPVRQLKNILFGKFPIYEIE
                     AHPLNRLGEATGKLIGGNLSVLMGLRGSNFDYEYNNAILFIEDIGEQLYQIDRMMQNL
                     RLSGILKRLAGLIVGQFTDCPEDPLMMQTIEEIIFEAAKEYHFPICFNFPVGHINNNL
                     PLIIGTPITLKINKNSVKVTFRNKN"
     gene            complement(4803..6518)
                     /locus_tag="PLR72_05270"
     CDS             complement(4803..6518)
                     /locus_tag="PLR72_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36644.1"
                     /translation="MKKCYLLLFLICLTEFVLAQDIPEHISYTRIYDFLDELANEKII
                     NINSAIKPYSRQFIAKKLQEAQANEKKLNKRQQNELYFFLEDYALEQNRLPDYDFPIE
                     DNRLWRLDILPPSLHYCDSLFHARVMPILGMNILYNANDKIAQRWYGFDFQSMIGNHF
                     AVYASLRDVSQNGELLAEPTYLTPYQAGAYKRTNNGGDYSEMRGGISYAWKWGDVALV
                     KDHIQWGDNYHGSNIISGRTPSFPMLKLSLKPAKWLEINYFHGWLTSNVIDSTRYYLS
                     NQNEKMYRYTHKFLAANMITFIPMPQLYLSAGNSIVYAESNVQPAYLIPIAFYKSIDH
                     TLTMGTENQNSQLFFDISSRNIKHLHLFTSIFIDEFSVARLKASNKEKNPISYKLGFR
                     LSNFPLDNLSLTGEFTKNAILTYKHSIPALTWATNGFNLGNYLGDNSEEIYVALQYKP
                     LRGLDINLSYTQAEHGNEYAYIRHGIWEGSKASVRDIISQPFMKDKIWSNKTLSFDIK
                     YEIFPNIYAILNIENSNIQAFEATSEVTFGEIRMTAEETLNFYTPKFLQGKQTTITAG
                     LSFGF"
     gene            complement(6682..6813)
                     /locus_tag="PLR72_05275"
     CDS             complement(6682..6813)
                     /locus_tag="PLR72_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36645.1"
                     /translation="MPENKEAFKELGYEYLAERKKKNRLEYLKRELKEHGYKIEKVA"
     gene            complement(6806..>7197)
                     /locus_tag="PLR72_05280"
     CDS             complement(6806..>7197)
                     /locus_tag="PLR72_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOK36646.1"
                     /translation="YQQHQLQNGGKFNKDFRITTLGRFMRKYWLDELPMLINLFKGDL
                     KLVGVRPLSPQYFNLYSKDLQELRLKYKPGLLPPLFADLPQTLEEIQLSELKYLSACE
                     KNGRFKTDCSYFFKIINNILFKKVRGA"
BASE COUNT         2452 a         1087 c         1206 g         2352 t
ORIGIN      
        1 atatgtgata tttttaacaa gaaaagttta ttggagaaat tgatatttgg acggtttaat
       61 taaaaaaata ttactataac gttttcaagt gcgtaaaaaa tggtctgaaa aagaagagct
      121 aataagttgg agtagagaat ttttcaaaga aataaaaaat taagttcaat ataacaatta
      181 aaggaaagga aaagaaacaa attcgcatat tttaatttta ttttaatata cttttttgca
      241 taactatacg tttctttttt agaattaaat taaaaaacta ttgtgcttat gcaaaaaaaa
      301 tctacttcaa agaaaaaaac taaattgagc aaaagttcat tcactgttgg tggattcgaa
      361 cttcatccac aaaaaaagac cattaagacg attcttcaat ttgcttcttc ttacagaact
      421 caaaaagtaa ataatacaaa ttttattgct ttgaacttaa attaaaaaaa gaagtttttc
      481 agaaagaatc gactttgacc tcctgaaaaa aaggagttta atggtttttt aatgttttta
      541 ctttcacttt tcttcttcct tgctgaacca taatgcatta atttttcaaa caggaaggaa
      601 tttaagaatt tttgtaggtt tgaaaaatgg ttgtaatttt accttctgat tttataatcg
      661 gatgattttt aatccgtaaa ttcaagttat atttttacaa ggaaaagaat ggaagagaaa
      721 aaattaacca ttcgggagtg ggctgaggaa gacagaccgc gtgaaaaaat gattcgaaaa
      781 ggcattcaaa gtctttcgga tgcagaatta ctggctattc ttattggttc aggcaataaa
      841 aatgaaagtg cggtggaatt atcaagaaga attttacacg aatgcaaaga taacttaaat
      901 gagttggctc aacttagtat agatgatttt tgtaagcgtt tcaaaggaat cggtactgcc
      961 aaagcagtca ccattatggc tgctcttgaa ctgggcaaaa ggcgaaaaac cactgaaatt
     1021 atcgaacgtg aaaaaataac gagcagtcat gatctttgta aactatttga acctatgctg
     1081 attgatttgc cccatgaaga attttgggtt gccattctca atacagccaa taaagtaatg
     1141 gaagtttttc gccttacaca aggtggcacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnna taaactttta ttttctcctg caattaatta aaaataatca ttaccaaaaa
     1321 tggcgtttct tgagatagaa aatatagtaa aaaaatatga gcatcaagta gctttaaatg
     1381 gagtcagcct gaatgttcct gaaaattcaa tttatggttt attaggtcca aatggtgcag
     1441 ggaaaaccac acttattcgc atcattaatc gaatgattgc tcctgatagc ggcgaagttc
     1501 gattgaacgg taaaataata aaagacgatg atttagtatt gttgggttac atgcccgaag
     1561 aacgaggctt gtataagaaa atgaaagtag gtgaacaagc tttgtttttt gctcaactga
     1621 aaggaatgaa aaaaggggat gcattaaaag ctttaaaata ctggtttgag aaatttgaaa
     1681 ttcaatcgtg gtggaacagg aaagtagaag aactgtcaaa aggaatggca caaaaagtac
     1741 aatttatcat caccgttctt catcaaccga aattattgat attagatgag ccattcagcg
     1801 gatttgatcc tatcaataca gacattatca aacaggaaat tttacaactt aaaaataatg
     1861 gcacgacggt aattttttct acacataata tgtcttctgt agaagaactt tgcgaaaata
     1921 tttcattgat tcatcaagca aagattattc ttcaagggaa agtatctgat atcaaagcct
     1981 cctattcaaa tagaactttt aaaattcgct ccttacaacc tattagtgaa aatgaacatt
     2041 tcaagctgat tacacaaatt caaaaagatg gaatgataga aagcattata aaaaagaaaa
     2101 cgaatgaaag taacaatgtt ttactttcag aacttataca acaaacagaa atcgtagctt
     2161 tcgaagaact attgccgagt atgaatgaca ttttcattga aaccgtacaa aaagaagaat
     2221 taaattaaac ttttgaagat aaactatccg tgaattttct gcaaatatct gttcagttat
     2281 gcccaataaa attcaattga ttatccgtcg tgaatacaca actcgtgtaa tgaagcgttc
     2341 atttttgctt cttacttttt taacaccttt actttttgcc gcattaatca ttgttccgtt
     2401 gtggttatct actttaaaaa cagaaacaaa aaaacatatc ttggtgattg acagaaccca
     2461 tcaatatgaa aaagccttaa aaaataatga ttcatatatt tttgagtttg tagataaacc
     2521 tttgaacgaa attcaaaaaa acacaaataa aaataaagaa ttagcaggtg tattggttat
     2581 cacaggtgat ttgtccgaaa attcaaatgc agtaacgctt tattctgaaa aacaaatcaa
     2641 tttagaaaca aaaaactata ttgctgagtt attaaatgat tatgtaaaag agcagaaatt
     2701 aagaaattat aatgttcctg atttagagaa aattattgaa aataccaatc aaaaaattga
     2761 tattgctact gtccgctgga atgaaaaagg cgaagaaaaa ttgagttcca cagaattgac
     2821 tattgccatt ggcatctttt cagccatgat ggtttacatt tttattctga cttatggttc
     2881 acaggtaatg agtggagtta ttcaggaaaa aaccaatcgc attgtagaaa ttattatttc
     2941 gtcggtgaaa ccttttcagc ttatgatggg taaaatcatt ggcattgcat tggtagggtt
     3001 gacacaattt tttatctgga tgattttgac acttgtaatt gtaggtattg ctggaagttt
     3061 atttaataca aatattgatt tcaattcact tacgcaatca aatgaaataa taaagaatga
     3121 acagattgta aatcaggcag tttcttcatc agaagctgaa aatgcgatga atcatttcct
     3181 tgaaatgttg caaggtgttg attttatgca aatcatcact ctttttattg tttattttat
     3241 tggaggctat ttgctatatg cttccctttt tgcagcagta ggttcggcag ttgacagtga
     3301 aaccgacaca caacaattta ctttgccact cacattgccc attatttttg ccatttatgc
     3361 agctatttac agtacacaaa atcctgatgg accgttggct ttctggtgtt cgctgattcc
     3421 ttttacttcg ccagtcgtga tgatggttcg cttacctttc ggagtagcaa cgtgggaaat
     3481 tttgctgtca ctggtcattt taattctttc attcataggt actacgtggt tagcaggaaa
     3541 aatttatcgt actggcattt taatgtatgg taaaaaagct acctggaaag aattatggaa
     3601 atggatgaaa tattaacttt tgattcgctt tataaaaatt gaaaatagaa aaatcaaata
     3661 taaaccttat ttaatttcct tttaaatgtc aattaaatga ttccaccatt tttaaaatca
     3721 ggagatcgac tccaaattat ttctccggct tcttgcattg accagagtta cattgaagga
     3781 gccaaaaaag ttttatcttc gtggggctta gaagtagccg aagggaaatt tgctcgaaat
     3841 gtttatggac gatttgccgg gacaaaagaa caacgcataa atgatttgca gaaagcattg
     3901 gatgatccta ctctcaaagc aatcttatgc agtcgtggtg gttatgggct ggctcaaatt
     3961 attgataaaa ttgatttcag caattttatc aaatttccaa aatggcttat tggcttcagt
     4021 gacattagta ttttacacgc tgctattcaa caatacggga ttgtttctat tcatggcata
     4081 atggcaaagc aactcactga actcgatcca ctctcagaac cagttagaca attaaaaaat
     4141 attctttttg gaaaatttcc tatttatgaa atagaagctc atcccttaaa tcgtttgggc
     4201 gaagccacag gcaaactcat tggaggaaat ctttcggtat tgatgggctt acgaggaagc
     4261 aattttgatt atgaatacaa caatgctatt ttgtttattg aagacattgg tgaacaactt
     4321 tatcaaattg acagaatgat gcaaaatttg cgattgagtg gcattttaaa aagattggca
     4381 ggtttaatag ttggccagtt tactgattgc cctgaagatc cgctgatgat gcaaactatt
     4441 gaagaaatca tttttgaagc agcaaaagaa tatcactttc ccatttgttt taattttcca
     4501 gtcggacata taaacaataa tttaccatta ataataggca ctcctattac gttaaagatt
     4561 aacaaaaaca gcgtaaaagt gacttttcgg aataagaatt gattttttaa atagttaata
     4621 attttaggaa gaggaaattt tttaggataa cattttaatc aaaagaattc ctcgaggctt
     4681 gcctcgtggt tattcatttt ttccagatac taaaaacaaa aacatttaga atgattttca
     4741 atattatttt atagactttt catttaagtc ataatactta atttttaatg acaaattttg
     4801 gttcaaaatc caaagcttaa acccgctgta atagttgttt gtttcccttg taaaaattta
     4861 ggtgtataaa aatttaaggt ttcttccgct gtcattctta tttctccaaa agtgacttca
     4921 gaagttgctt caaaagcctg tatattgctg ttttcaatat ttaaaatggc ataaatattt
     4981 gggaaaatct catattttat gtcgaatgat aaagttttat tgctccagat tttatctttc
     5041 ataaatggtt gactgataat atcgcgaaca cttgctttcg aaccttccca gattccatgt
     5101 cgaatgtagg catattcatt accatgttct gcttgtgtgt atgataaatt tatatccagc
     5161 ccacgtaaag gtttatattg caaggcaaca tatatttcct ctgaattgtc gcccagataa
     5221 tttcccagat tgaaaccgtt tgttgcccat gtcaaagccg ggatggaatg tttatatgtt
     5281 agaatagcat ttttggtgaa ctcaccagtc aacgaaagat tgtccagtgg aaaattactg
     5341 agacggaaac caagtttata ggaaatagga tttttttcct tattggaagc tttcaagcga
     5401 gctaccgaaa attcatcaat gaagatggaa gtaaataaat gtaaatgttt aatatttctt
     5461 gaactgatgt caaaaaacaa ttgagaattt tgattttctg tgcccatagt cagtgtatga
     5521 tctattgatt tataaaaggc tatcggaatc agataagctg gttgcacatt actttcggca
     5581 tacacaatag aattccccgc agatagatat agttgaggca taggaataaa agttatcata
     5641 ttggcagcga gaaatttatg agtgtatcgg tacatttttt cattttgatt agaaagatag
     5701 taacgtgtgc tatcaatcac attggaagtt agccaaccat gaaaataatt tatttccaac
     5761 catttggcag gttttaaact tagtttcagc atggggaaag aaggagttcg acctgaaata
     5821 atatttgaac catgataatt gtcgccccat tgaatgtgat ctttgaccaa tgctacatcg
     5881 ccccatttcc aggcataaga aattccacca cgcatttcgc tgtaatcgcc accattattg
     5941 gtacgtttgt aagctcctgc ttgatatggt gttaaataag taggctctgc taataattca
     6001 ccattttgag aaacatccct gagcgaggca taaactgcaa aatgatttcc aatcatcgac
     6061 tgaaaatcaa atccatacca acgctgtgca atcttatcgt tggcattata aagaatgttc
     6121 attcctaaaa taggcattac tcgagcatgg aaaagagaat cgcaataatg caaggaagga
     6181 ggaagaatat ctaatctcca aagtctatta tcttcaattg gaaaatcata atcaggtaaa
     6241 cgattttgct ccagtgcata atcttcaagg aagaaatata gctcattttg ttgcctttta
     6301 tttaattttt tttcatttgc ctgtgcttcc tgtaattttt tagcaataaa ttgtcgagaa
     6361 tagggtttta ttgctgaatt aatattaatt attttttcat tagctaactc atcgagaaaa
     6421 tcgtaaatgc gagtgtaaga aatatgttct ggaatatctt gagcgagcac aaactctgtt
     6481 aagcaaatta aaaacagcaa caaataacat tttttcatcc gtaaataatt attaaattaa
     6541 acaatatcag catattgaaa aacagataag gagttaaaat ttcatccgcg aactcgaagt
     6601 tcatgtgttt gtgcttatct gttgatgtca gtttcataag cgggggttaa ccaccagtaa
     6661 aaaacgacct tttgcttttc tttatgctac tttttctatt ttatatccgt gctcttttag
     6721 ttctcgtttc aaatactcca aacggttttt cttttttcgt tctgccaaat attcgtaacc
     6781 aagttcctta aaagcttctt tattttcagg caccacgaac ttttttaaag agaatattat
     6841 ttataatttt aaaaaaataa gaacaatcgg ttttgaatct cccatttttt tcacaagccg
     6901 atagatattt tagttctgaa agttgaattt cttccagtgt ttgtggtaaa tctgcaaaga
     6961 gaggaggtaa aagacctggt ttgtatttta aacgtaactc ctgtaaatct ttgctatata
     7021 aattaaaata ttgaggactt aatggccgga cacctaccaa ttttaaatcc cctttaaaaa
     7081 ggttaattaa catgggtagt tcatccagcc aatacttacg cataaatctt cctaaagtgg
     7141 taattctgaa atctttatta aatttccctc cattttgaag ttgatgttgt tgataaa
//
LOCUS       DAOSAS010000091         7189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6675_length_7189_cov_12.929913, whole genome shotgun sequence.
ACCESSION   DAOSAS010000091 DAOSAS010000000
VERSION     DAOSAS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6675_length_7189_cov_12.929913"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLR72_05285"
     CDS             <1..430
                     /locus_tag="PLR72_05285"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOK36647.1"
                     /translation="DVNIIKQLLQEVRPHQIYAAADLADPHGTHKICLDAVLAAIDEL
                     KEEEWMKDCWIWLYRGAWKEWEIDHIEMAVPLSPEELRNKRNAILRHQSQMENAPYLG
                     DDQRLFWQRSEDRNRATAELYTKLGLASYEAIEAFVRYII"
     gene            458..1930
                     /locus_tag="PLR72_05290"
     CDS             458..1930
                     /locus_tag="PLR72_05290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOK36648.1"
                     /translation="MKIIDCFIFYEEIEGIKKTIQNLLESELVNNIYLLTKSSLNPNV
                     EKCDNIPISSLMSTDTVKKIADKSQALYTMIYLKNTTLELGQFSLERFYQIANDTQAG
                     LIYSDYYQITNGTRSKLPLIDYQLGSVRDDFNFGSVLFYNTECLKKAVSEFSENYQYA
                     GFYDLRLKVSQCTELIHIDEYLYSDVELDIRKSGEKQFDYVDPKNREIQLEMEKACTD
                     HLKKIDAYLKPVFKKISFNEEKFPVEASIIIPVKNRVTTIEDAIRSALSQKTTFEFNV
                     IIVDNYSTDGTSQIIAKYAKKDERIIHVIPEREDLLIGGCWNLAIHHPLCGKFAVQLD
                     SDDIYQDENTLQTIVEAFYEQNCGMVVGSYTTTDFNMNILPPGLIDHKEWTPENGRNN
                     ALRVNGFGAPRAFYTPLLRKIKVPNSNYGEDYALGLVFSRDYQIGRIYRSLYLCRRWK
                     DNSDAELDPATLNLYNHYKDKIRTIEIKARQQKNKLELNK"
     gene            complement(2052..3386)
                     /gene="gdhA"
                     /locus_tag="PLR72_05295"
     CDS             complement(2052..3386)
                     /gene="gdhA"
                     /locus_tag="PLR72_05295"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOK36649.1"
                     /translation="MNVEKLLSSLEAKHPGEKEYLQAVREILTSIDEIYSQHPEFEKV
                     KIVERLVEPDRIITFKVPWIDDEGNVQVNIGYRVQFNNAIGPYKGGLRFHPSVNLSIM
                     KSLAFEQIFKNALTTLPMGGAKGGSDFNPKGKSEMEMMRFCQSFMTELWRYLGPDIDV
                     PAGDIGVGGREIGYLYGMYKKLTRENTGVLTGKNLEFGGSYIRPEATGFGALYFVQQM
                     LKTNGMDIQGKTIAVSGFGNVAWGVILKAAQMGAKVVTVSGPDGFIYDENGITGEKID
                     YLLELRASCNDIVSPYAEKFGVPFFPNRKPWEVKVDIAMPCATQNELNKDDAQMLINN
                     HVKCVAEVSNMGCTPEAIDLFLQHKLLFAPGKAVNAGGVSVSGLEMTQNAIHLNWTAE
                     EVDAQLHQIMKKIHEQCIKYGRENDGYVNYLKGANVAGFMKVAKAMLAQGII"
     gene            complement(3915..3988)
                     /locus_tag="PLR72_05300"
     tRNA            complement(3915..3988)
                     /locus_tag="PLR72_05300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3952..3954),aa:Arg,seq:tct)
     gene            4235..6670
                     /gene="thrA"
                     /locus_tag="PLR72_05305"
     CDS             4235..6670
                     /gene="thrA"
                     /locus_tag="PLR72_05305"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOK36650.1"
                     /translation="MKVLKFGGTSVGSVQGILNLKKIVESQNEQIVVVVSALSGVTDQ
                     LYHISKIAASGDESYLKEYDEMFRRHMEIIHQVISPEKKAKTVSQVQTLFADLLNIFR
                     GIYLIKDISTKITDTVVSYGEMISSVIIANVIKNAVHFDSRTFIKTKDQYNKHMVDFK
                     ATNALIQEKFKDAPQIVVCGGFISTDVEENYVTNLGRGGSDYTSAILAAALNASILEI
                     WTDVDGFMTADPHIINNAYVIDRLSYSEAMELCNFGAKVIYPPTIFPAFHKSIPIIIK
                     NTFHPDAQGTYISKEKSGEPSKAIKGISSIDDTSLITIRGLGMVGVIGVNYRIFRALA
                     KNGISVFFVSQASSESTTSIGVRNADSALAVQVLKDEFIYELEQGEIDEIFSEDEMAT
                     IAIVGDNMKRTPGIAGKLFGTLGRNGINVVACAQGASETNISFVTDRSYLRKALNVIH
                     DSFFLSEYQVLNIFLVGIGMVGSNLLNQIKMQQPKLIQEKGLKLNVVGIATSEKMYFD
                     REGIDIEQYNEILETKGVVSSSEKIASEIINMNIFNSVFVDCTASEQIADLYQQLLDH
                     NISVVAANKIAASSSYDKYIRLKETALRKGIKFLFETNVGAGLPIISTINNLINSGDR
                     IFKIEAVLSGTLNFIFNTISEEIPLSKAIRLAKEKGFSEPDPRIDLSGKDVVRKLVIL
                     AREAGYRVEQEEVVKKLFIPDKFFKGTLEEFWESISELDEEFEQKRQILASQGKRWKF
                     VASMDHDYCEVGLQEVDQHHPFYDLQGSNNIILLTTERYNEFPMMIKGYGAGASVTAA
                     GVFSDIISIANIR"
     gene            6675..>7189
                     /locus_tag="PLR72_05310"
     CDS             6675..>7189
                     /locus_tag="PLR72_05310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOK36651.1"
                     /translation="MKNTRVLFSVVFSLCFVIACKKMPPQLPANKNVDDVQNTMSMME
                     INHQLALREDSLLQVYVANLKDKSFRKTETGLWYKIEKTKQGKKIKNNDVCQFSYELF
                     SLDDKLLEKNEQEIIVIGKKQTIKGLEEGLQLLRKGDNAIFIIPWYLAYGMKGKEPLI
                     APYVSVIYRVKV"
BASE COUNT         2452 a         1073 c         1288 g         2376 t
ORIGIN      
        1 agatgtaaat attattaaac aacttttaca agaagttcgt ccgcatcaaa tttatgcggc
       61 tgccgactta gccgatcctc atggaacgca taaaatttgc cttgatgctg ttttagcagc
      121 tatcgacgaa ctcaaggaag aagaatggat gaaagattgt tggatatggt tgtatagagg
      181 tgcatggaaa gaatgggaaa tagatcatat agaaatggct gttcctttaa gtcctgaaga
      241 attgagaaac aaacgaaatg ctatcttacg gcaccagtca cagatggaaa atgcccctta
      301 tttaggtgat gatcaacggc ttttttggca gcgttctgaa gatagaaatc gtgcaactgc
      361 tgaattatat accaaattag gcttggcttc ttatgaagcg atagaagctt ttgtcagata
      421 tattatataa taaaaaaata ttaatgctta catagttatg aaaatcatag attgttttat
      481 tttttatgaa gagatagaag gaataaaaaa aactatccag aatttattag aaagtgaatt
      541 ggtaaataat atttatcttt taacaaaatc ttctcttaat ccgaatgttg aaaaatgtga
      601 caatattcca ataagttcct tgatgagtac ggatacagta aaaaaaattg cagataaatc
      661 gcaagctctt tatacaatga tttatcttaa aaatacaaca ttggaattag gtcaattttc
      721 tttagaacga ttttatcaaa tagctaatga tactcaagct gggttgattt attctgatta
      781 ttatcaaata accaacggaa ctcgttctaa attaccactg atagattacc aattaggtag
      841 cgtacgagat gactttaatt ttggttctgt attgttttac aacactgaat gtttaaaaaa
      901 agctgtgagt gaattttctg aaaattatca atatgctggt ttctatgatt taaggttaaa
      961 agtttctcaa tgcaccgaat tgattcacat agatgaatat ttatactctg atgtagaatt
     1021 agatattcgt aaaagtggtg aaaaacaatt tgattacgta gatccaaaaa atcgtgaaat
     1081 tcagttggaa atggaaaaag catgtactga tcatttaaaa aagatagatg cttacctaaa
     1141 acccgtattt aaaaaaattt catttaacga agaaaaattt cctgttgagg cttccataat
     1201 tattcctgtc aagaataggg tgactaccat tgaagatgct attcgctctg ctttgtcaca
     1261 aaaaaccact tttgaattta atgttattat tgtggataat tattcgacgg atggaacaag
     1321 tcaaataatt gccaaatatg caaagaaaga tgaacggatt attcatgtta tccctgaaag
     1381 agaagattta ttgataggag gttgttggaa tctggcaatt catcatcctt tatgtggtaa
     1441 atttgctgtt caactggata gtgatgatat ttatcaggat gaaaatactc tacaaactat
     1501 cgtggaagct ttttatgagc aaaattgtgg tatggtggta ggttcttaca ccacaaccga
     1561 ttttaatatg aatattcttc cgccggggct gattgatcat aaggaatgga ctcctgaaaa
     1621 cggtcgtaat aacgctttaa gagtgaatgg ttttggcgct ccacgagctt tttatactcc
     1681 tttattaaga aaaattaaag ttcctaattc taactacgga gaagattatg cattgggact
     1741 tgttttcagc cgggattatc agataggaag aatttatcgt tcactttatc tttgtcgtcg
     1801 ttggaaagat aattctgatg ccgaattaga tcctgcaaca ctaaatcttt ataatcatta
     1861 taaggataaa atcagaacta tcgaaataaa agcaagacag caaaaaaata aactggaact
     1921 taacaaataa atcaaactta gttccggaaa gtataaagta gcgatttttt aaagtagcat
     1981 tttgttgatt tctaatcagt gcttttattt gaaaaacact gattagaaat tataaattgg
     2041 aactaaaaaa attatattat tccttgtgca agcatagctt ttgcaacttt cataaaacca
     2101 gccacattag ctcctttcaa ataattaacg tagccatcgt tttcgcgtcc atattttatg
     2161 cattgttcat gaattttttt catgatttga tgtaattgag catccacttc ttcggctgtc
     2221 caatttaagt ggatagcatt ttgtgtcatt tccaaacctg aaacgcttac tccgcctgca
     2281 tttactgctt ttccgggagc aaataacaat ttatgttgta aaaataaatc aatagcttct
     2341 ggcgtacacc ccatattaga aacttctgca acgcatttaa catgattatt aatcaacatt
     2401 tgagcatcgt ctttatttag ttcattttgt gtagcgcaag gcatggcaat atccactttt
     2461 acttcccacg gcttccgatt tgggaaaaat ggaacgccaa atttctcagc ataaggtgat
     2521 acaatatcat tacaacttgc tcttaattcc agcagataat caattttttc acccgttatt
     2581 ccattttcat cataaataaa gccatccggt ccagaaacag ttaccacttt ggcacccatt
     2641 tgcgcagctt ttaaaataac accccaagct acattaccaa aacctgaaac agcaattgtt
     2701 tttccttgaa tatccatacc attggttttg agcatttgct gaacaaaata caaagcacca
     2761 aaacctgtgg cttctggtcg aatgtaagaa ccgccaaatt ctaaattttt gcctgttaaa
     2821 actcctgtat tttcacgagt taactttttg tacattccgt ataaataacc aatttcacga
     2881 cctccaacac caatatcacc tgctggcaca tcaatatcag gtccaagata acgccaaagt
     2941 tcggtcataa aagattgaca gaatcgcatc atttccattt cagatttccc tttcggatta
     3001 aagtcagaac ctcctttagc tccacccatt ggtaaagttg ttaaagcatt tttaaaaatt
     3061 tgctcaaaag caagcgattt cataatagat aaatttactg aaggatgaaa ccgcaaaccg
     3121 cctttataag gtccaatggc attgttgaat tgcacgcgat aaccgatatt tacttgcaca
     3181 tttccctcat catctatcca gggaactttg aaagtaataa ttcgatctgg ttctaccaaa
     3241 cgttcaacaa tttttacttt ttcaaattca ggatgttggg aataaatttc atcaatacta
     3301 gttaatattt cacgaactgc ttgtaaatat tctttttctc cgggatgttt agcttcaagt
     3361 gatgataata atttttcaac attcataata tttatttttt agaaattagt aattatattt
     3421 ataatatgat aaatatcctt gttaaggaaa atgaattcaa aaatataaat aagaaagtgt
     3481 agttttttcg ggtgcaaatt taaacttttt atttcatatt gcaaacattt gatagaaatt
     3541 tttatcattt gaataataaa attttaaaaa agaaagtaat tatctactaa taaatgattt
     3601 ttagaagaaa tatgttttaa ttgatttgaa taaaaggaag ttcaaaaaaa atgattgttg
     3661 ggtgagtgaa ccatagtata cattttttca aaattcagct ttttggaaat gttttttact
     3721 atttagtttt tataaaaaaa gatgataatt gattattaat ctggcagttt actattgtta
     3781 aatacacttg ccatatacat tgttgttaaa gccgttgttg tcaaatgtag aaaagggtgg
     3841 ataagaaaag taggaatgac aataaaacct cacaaatggc ttccaggaga agaaatcttt
     3901 tattgatgga ggggtgatct cggcgggatt cgaacccacg acccacagct tagaaggctg
     3961 ttgctctatc caactgagct acgagaccag aattttttgg tgtaaaaaaa gaaaaacaaa
     4021 attaatcagc atcaagaaat cattaggaat gcaattttta ttattttcga ttgattttcc
     4081 tgctatttcc cgttgcaaaa gtactgtaat tttaaaaaaa acgcaagtgt ttttattcaa
     4141 aattcataat aaggagtctt tttcgactaa aaataactaa ttttgtaaaa ttaaaaggta
     4201 ttattatcaa aataataaat atatagactg ataaatgaaa gtattaaaat ttggcggcac
     4261 ttcggttggc tcagtgcagg gcattttgaa tttaaaaaaa attgttgaat cacaaaacga
     4321 acagatagta gtagtggttt ctgctttgtc aggtgtaaca gatcagttat atcatatttc
     4381 aaaaatagct gcgagtggtg atgaaagtta cttaaaggaa tatgatgaaa tgtttcgccg
     4441 tcacatggaa atcattcatc aagtgatttc tcctgaaaaa aaagcaaaaa cagtcagtca
     4501 agttcagaca ttgtttgctg atcttttaaa tatatttcgt ggaatttatt taattaaaga
     4561 tatatccact aaaattactg atactgttgt aagttatggt gaaatgattt cttctgtcat
     4621 tattgccaat gttataaaaa atgctgttca ttttgattca cggacattta ttaaaacaaa
     4681 ggatcaatat aataaacaca tggtagattt taaagctacc aatgctctta ttcaagaaaa
     4741 atttaaggat gctcctcaga ttgtagtatg tggcggattt atttcaaccg atgtagaaga
     4801 aaattatgtt actaatttgg gtagaggagg ttctgattat acttctgcta ttcttgctgc
     4861 tgccttaaat gcttccattc ttgaaatctg gacagatgtt gatggtttta tgactgccga
     4921 tccacatatc attaataacg cttatgtaat tgatcgattg agttatagtg aagcaatgga
     4981 gctttgtaat tttggtgcaa aagtgattta tccacctact attttccctg cttttcataa
     5041 aagtattcca atcatcatta aaaatacttt tcatcctgat gcacaaggca cttacatttc
     5101 gaaagaaaag tcgggtgaac cttccaaagc cattaaagga atttcatcca tcgacgatac
     5161 ttccttaatt accattcgag gtttgggaat ggtaggtgtt attggagtta attaccgaat
     5221 ttttcgtgct ttggcaaaaa atggtatcag cgtatttttt gtttcgcagg cttcttctga
     5281 aagtactact tctatcggtg ttcgaaatgc tgatagtgca ttagctgttc aagtgctgaa
     5341 agatgaattt atttatgaat tagaacaggg tgaaattgat gagatttttt ctgaagatga
     5401 gatggcaaca attgccattg ttggcgacaa tatgaaacgt actcctggta tagctggaaa
     5461 actttttggt actttagggc gaaatggtat taatgttgtt gcttgtgcac agggagcatc
     5521 ggaaactaat atttcatttg ttactgatag aagctatttg cgaaaggcat tgaatgttat
     5581 tcatgattcg ttttttctgt ctgagtatca agtgctgaat atttttttgg tgggtattgg
     5641 tatggtggga agcaatttgt taaatcaaat caagatgcag caacccaaat tgatacagga
     5701 aaaaggatta aaattgaatg ttgtaggaat agctacaagt gaaaaaatgt atttcgacag
     5761 agaaggaatt gatattgaac aatataatga gattttggaa acaaaaggtg tagtatcttc
     5821 ctctgaaaag attgctagtg aaattatcaa tatgaacatt tttaattctg tttttgtgga
     5881 ttgtactgcc agtgaacaaa ttgctgattt gtatcaacaa ttactcgatc ataatatttc
     5941 tgtggtagca gctaataaaa tagcagcctc ttcttcgtac gacaaataca ttcggttgaa
     6001 agaaactgct cttcgtaaag gaattaaatt tctttttgaa accaatgtag gagctggttt
     6061 acctattatc agcaccatta ataatttgat aaatagcggc gaccgtattt ttaaaattga
     6121 agcggtactt tccggaacgt tgaattttat ttttaatacc atcagtgaag aaataccttt
     6181 gagcaaagct attcggttgg cgaaagaaaa aggtttttca gaacctgatc ctcgtattga
     6241 tttaagtggt aaagatgtgg tgcgaaaact tgttattctg gcacgtgaag caggctatcg
     6301 agtggaacag gaagaagtag taaaaaaatt atttatccct gataagtttt ttaagggaac
     6361 attggaagaa ttttgggaaa gtatttccga actggatgaa gaatttgaac aaaaaagaca
     6421 gattttggct tcgcagggaa aacgttggaa atttgtagct tctatggatc atgattattg
     6481 tgaagtggga cttcaggaag tagatcagca tcatcctttt tatgatttgc aaggaagtaa
     6541 taatatcatt ttacttacca cagaacgtta taatgaattt cctatgatga tcaaaggtta
     6601 tggagctgga gcaagtgtta ctgcagcagg agtattctcc gacattatca gcattgctaa
     6661 tattcgttga gagaatgaaa aacactcgag ttttattttc tgtggtgttt tctttgtgtt
     6721 ttgttattgc ttgcaaaaaa atgccaccac aattaccagc caataaaaac gttgatgacg
     6781 tccagaatac tatgtcaatg atggaaataa accatcaatt agcacttcga gaagacagct
     6841 tattgcaagt gtatgtggcg aacttgaaag acaaaagttt caggaagaca gaaactggat
     6901 tgtggtataa aattgaaaaa actaagcaag gaaagaaaat aaaaaataat gatgtttgtc
     6961 aattttctta tgaacttttt tcattggatg acaagttgct tgagaaaaat gaacaagaaa
     7021 ttattgttat aggaaaaaaa cagactatca aaggacttga agaaggattg caacttttac
     7081 gcaaaggcga taatgccatt ttcattattc catggtatct ggcttacggg atgaaaggga
     7141 aagaaccatt aatagctcct tatgtttcag taatttacag ggtaaaagt
//
LOCUS       DAOSAS010000092         7182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6685_length_7182_cov_12.559282, whole genome shotgun sequence.
ACCESSION   DAOSAS010000092 DAOSAS010000000
VERSION     DAOSAS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6685_length_7182_cov_12.559282"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(522..779)
                     /locus_tag="PLR72_05315"
     CDS             complement(522..779)
                     /locus_tag="PLR72_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36652.1"
                     /translation="MEKACDLDVYKNSAFFCILDEQSKKLLEKTRKAMFCKGNLITVY
                     NHTGIQKYSDMSILSEIGNDMSSFGKASQFGGLGWLTSKKQ"
     gene            977..1336
                     /gene="rsfS"
                     /locus_tag="PLR72_05320"
     CDS             977..1336
                     /gene="rsfS"
                     /locus_tag="PLR72_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOK36653.1"
                     /translation="MEENEQIVEKIVEGIQERKGKNIVIINLTKLNDAPCSYFVICEG
                     TSNIQVRAIGDSIKNYVLNKLRVKPYATEGYKNSEWIAVDYGFVIVHIFQPRVRTFYD
                     IEHLWEDADIIEIEDVL"
     gene            1411..3459
                     /gene="ftsH"
                     /locus_tag="PLR72_05325"
     CDS             1411..3459
                     /gene="ftsH"
                     /locus_tag="PLR72_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOK36654.1"
                     /translation="MDNKFQQNKPQNPGPKTPKFNISWIYAIIIAILIGSYLFTDTSP
                     VKEIPYSDFKSYMNTQKIEKIAIYSSRNVAEAQIVKDSLKEVFGDKYEMYAKERKVRT
                     RIPSVEVFSSDYQKAQDDYGLNAVVEYKESKNYIDIFLYSILPFLLLILFFVFMNRRM
                     AKQMGGSNSGGGIFSVGKSKAQLFDKNTSNRITFKDVAGLAEAKQEVEEIVSFLKNPS
                     KYTELGGKIPKGVLLVGPPGTGKTLLAKAVAGEADVPFFSISGSEFVEMFVGVGASRV
                     RDLFRQAKEKAPCIIFIDEIDAIGRARGRNPNFGGNDERENTLNQLLTEMDGFASNSG
                     VIILAATNRADVLDKALLRPGRFDRQIHVDLPDVNDRKEIFKVHLRPIKINKTVDVDF
                     LAKQTPGFSGADIANVCNEAALIAARKNKTFVDKQDFLDAVDRIIGGLERRNKITTKS
                     EKRSIAYHEAGHATISWMLEHANPLVKVSIVPRGEALGAAWYLPEERQLTVREHMLDE
                     MCATLGGRAAEEVFLNQVSTGAINDLEQVTKRAYAMVTYFGMSEKLPNLCYYDSTGSS
                     EFSFTKPYSEKTAELIDAEVKKIVNEQYLRAKEILKKYAEGHNKLAELLIEKEVIFAE
                     DLEKIFGKRIWASRSDELLAQNSENQSSQKNEESKSDENLEENTQETSTKASAFQQIK
                     "
     gene            complement(4234..5247)
                     /gene="dusB"
                     /locus_tag="PLR72_05330"
     CDS             complement(4234..5247)
                     /gene="dusB"
                     /locus_tag="PLR72_05330"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOK36655.1"
                     /translation="MKIGNTDFPNYPLFLAPMEEVTDPPFRLLCKQFGADLVYTEFVS
                     SDALIRKIERSLQKITFFESERPIAIQLYGQHVDAMVQAALMAEELQPDIIDLNFGCP
                     VKKVANKGAGAGLLKDIPKLLEITKAVVKAVKLPVTVKTRLGWDENNKIILTLAEQLQ
                     DCGIQALTIHARTRSQMYRGEADWTLIGEVKNNPRIKIPIIGNGDVTTPQKAKEYFDK
                     YGVDAIMIGRGSLGQPWIFKEMKEFLEKGEISSSLSFQQKREILKNYILSCIQWIDFK
                     NEEVNFETISNIRLKGIIHARCHLAATPIFKNIPNFKNTRIAMLRADTIQELFQIIDN
                     VNI"
     gene            5633..6247
                     /locus_tag="PLR72_05335"
     CDS             5633..6247
                     /locus_tag="PLR72_05335"
                     /inference="COORDINATES: protein motif:HMM:NF039701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HOK36656.1"
                     /translation="MKKAVFLFFSLLLITGWASSKNVAIDSVKPDVTVTKNLSKEVKD
                     SFLLQKLSPQQLIELKKAELENERKKIKAEAREDMPFTGFQLFLICVLPFLFVIVIIW
                     IIYYQKGKESKQRYELYLKSLELGQTLPEDFFEKKAKNKPNSDLKKGVLWLAVGLSLL
                     IYFLIVKNMGALILGIVPAFVGIGYLLVYFLEKPKSPDDTDKDE"
     gene            6240..6785
                     /locus_tag="PLR72_05340"
     CDS             6240..6785
                     /locus_tag="PLR72_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK36657.1"
                     /translation="MNNAEDLRWIAQVILSRDERAFEKLIRKYQSPIRRFFLNLTMGD
                     VFLSDDLAQETFIKAFLYLPTFKALSNFSTWLFRIAYNVYYDAVRARKDFYDLDEKEI
                     NQLYQVNNSYSTYKEDFYKALINLNEMEKIAVLLYYMEDKTQREIATIMKCPIGSVKN
                     YIRKGKEKIANFLKENGYGNE"
     gene            6772..7083
                     /locus_tag="PLR72_05345"
     CDS             6772..7083
                     /locus_tag="PLR72_05345"
                     /inference="COORDINATES: protein motif:HMM:NF027794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5056 domain-containing protein"
                     /protein_id="HOK36658.1"
                     /translation="METNETDKNFQDFFKLHKHDIADNGFASRTLRHLPQRKDNSWIV
                     WLFTTIGIATALYIGIFSDWLMNFIEHMFNLPFIYLLIGVALFPLVIGLCIMFWEKEQ
                     Y"
BASE COUNT         2467 a         1111 c         1251 g         2353 t
ORIGIN      
        1 agaagtttta tactgcttgt aagttacgtt attttttatt tttcctcctg taattgctcc
       61 tttttacatt ctttcaggtt tcaaattaca caaaagaacg tctcgatgct attcataagg
      121 catcagaatt tttacattct ttcaggtttc aaattacacc ccaattttta acataatcca
      181 aaattggtcc caatccaacc tcataccaag ttgttttcat taccggcaaa gtctgttgtt
      241 cattttgtgt acacgggata atatctttca atatttcgcc acaaaggtaa agaactgaga
      301 aaatgccgga agaaaccgct caaagagaga gatttctcaa tcagattttt attgcctgta
      361 aatttctatt gacgtcaaag gactttccgg tttgcaatac gttgaaaata ataacgagta
      421 ttttgcgggt tatagtcagt aatacctttt gcgatttcat ccgttttgag agatatttgt
      481 taccgtgcaa ggttttacgt gactgtattt tgccagccga ttcattgttt cttggacgta
      541 agccagccca acccaccaaa ttgggaagct ttaccaaaag aactcatgtc attgccaatt
      601 tctgagagaa tactcatgtc actgtacttt tgaatgcctg tatggttgta cacagtaatg
      661 agatttccct tgcaaaacat cgctttgcga gttttttcta aaagtttttt gctttgttcg
      721 tccaaaatgc aaaaaaatgc actattttta taaacgtcaa ggtcacatgc tttttccata
      781 aaattaccgg attaaattgt taaacaatta gtttatttca ttggttggta aaaaccaact
      841 tacaaaggta acatttgtgc tcagaagcac ggtacggtaa acttttaaag tcttgtgccc
      901 ttgattttta ttttcacaca tttggaaata cctcgatttt cttgtacttt tgtacaagga
      961 aaaactatca atttaaatgg aagaaaacga acaaattgtt gaaaaaattg ttgaaggaat
     1021 acaggaaaga aaaggtaaaa acattgtaat tatcaattta actaaattga atgatgctcc
     1081 atgcagttat ttcgttatct gtgaaggaac ttctaatatt caggtaaggg caattggtga
     1141 ttcgattaaa aattatgtat taaacaagct gcgtgtaaag ccctatgcaa cggagggata
     1201 taaaaatagt gagtggattg ctgtggatta tggatttgtt attgtgcata tttttcaacc
     1261 aagagtacgt accttttatg atattgaaca tctatgggaa gatgctgata taattgaaat
     1321 tgaagatgtt ttgtaataaa acaataataa ttaaaaaaaa acaataataa tttgatttat
     1381 ttagaaatat aagttaatgt tgatttaaaa atggacaata aatttcaaca aaataaacct
     1441 caaaaccctg gaccaaaaac tccaaaattt aatatatcgt ggatttatgc tatcattata
     1501 gctatactga ttggttctta cttgtttact gatacttcgc cagtaaaaga aattccttat
     1561 tcagatttca aatcttatat gaatacgcaa aaaatagaaa aaatagccat ttattcttca
     1621 cgaaatgttg ctgaagctca aattgtaaaa gattctctta aagaagtctt tggtgataaa
     1681 tatgaaatgt atgcaaaaga acgaaaagta aggactcgca ttccatcagt ggaagttttt
     1741 tcttcggatt atcagaaagc ccaagatgac tatggtctaa atgctgtggt agaatacaag
     1801 gaaagtaaaa attatattga tattttcttg tatagcatac ttccattttt attattgatt
     1861 cttttcttcg tgtttatgaa tcgccgtatg gcaaagcaaa tgggaggaag taacagcgga
     1921 ggaggaattt ttagtgtagg taaatcgaaa gcacagcttt ttgataaaaa caccagcaac
     1981 cgtatcactt ttaaagatgt ggcaggactt gctgaagcca aacaggaagt ggaagaaatc
     2041 gtatcatttt tgaaaaatcc aagtaagtat accgaattag gaggtaaaat cccaaagggt
     2101 gttttattgg taggtcctcc aggaacagga aaaacattac tggcaaaagc tgtagcaggc
     2161 gaagcagatg ttcctttctt ttctatttca ggttctgaat ttgtggaaat gtttgtagga
     2221 gttggtgctt cacgcgttcg cgatttattt agacaagcaa aagaaaaagc accatgcatt
     2281 atttttattg atgaaattga tgctattggg agagctcgtg gtagaaatcc aaattttggt
     2341 ggtaatgatg aaagagaaaa cacactgaat caattactta ccgaaatgga tggctttgct
     2401 tccaatagcg gagttattat tttggcagcc accaaccgtg ccgatgtttt agacaaagct
     2461 ttactaagac caggtcgttt tgatagacag attcacgttg atttaccaga tgtaaatgac
     2521 cgtaaagaaa tttttaaagt ccatttacgc cctattaaaa ttaataaaac agttgatgtt
     2581 gattttttag caaaacaaac acctggcttt tcaggtgctg atattgcaaa tgtttgtaac
     2641 gaagcggctt tgattgcagc acgaaaaaat aaaacttttg ttgacaaaca agatttcctt
     2701 gatgcagtag accgaatcat tggtggattg gaaagacgga ataaaatcac tacaaaatct
     2761 gaaaaaagat caatagctta ccacgaagca ggccatgcca ctatcagttg gatgttggaa
     2821 catgcaaatc cgttggttaa ggtaagtatt gtgcctcgtg gcgaagcatt gggagctgcg
     2881 tggtatctcc ctgaagaacg tcaattgacc gtccgcgaac acatgctgga cgaaatgtgt
     2941 gcaaccttag gcggtagagc agccgaagaa gtttttttga atcaagtttc aacaggagca
     3001 attaatgacc tcgaacaagt taccaaacgt gcctatgcta tggttactta tttcggaatg
     3061 agtgaaaaat tacctaattt atgctattat gattccacag gaagctctga attttcattt
     3121 acaaagcctt atagcgaaaa aacagctgaa ttgattgatg ctgaggttaa aaaaattgtc
     3181 aatgaacaat atcttcgtgc aaaagaaata ttaaaaaagt atgctgaagg gcataataaa
     3241 ttggcagaat tgctgattga aaaggaagtt atttttgcag aggatctgga aaaaattttt
     3301 ggaaaacgaa tttgggcttc aagaagtgat gagctccttg ctcaaaattc tgaaaatcaa
     3361 tcttctcaga aaaacgaaga gagtaaatct gatgaaaatc ttgaagaaaa cacacaagaa
     3421 acttctacaa aagcttctgc attccaacaa atcaaatagt atattttctt caggcaaaac
     3481 ttttgaaaaa tagaggaaat gaagcaaatc agaccattaa aaaatgaaat ctcgtttcat
     3541 tacattttat tttgataaag gtatctgcat tgcatattgg tgagtgctcg taaatttcta
     3601 ataaatattt aacaagctaa gatttacgag caatttaagt taaattaatc gtcatctttt
     3661 tttccttcat ttctactatt aaaggaccaa ataaaacatt tgatattgca aaaatgccgt
     3721 taggggcgtt cgcaatccca aagaattaat ttctaatttc gtaatttata cgtaattaaa
     3781 ccaaccatta aaatttaaaa attatcgcgt tatgtcaggc tccagaatgg gtattaaaat
     3841 accgtaagaa atggactaaa ctttgttttc taaatggata ttattatctc tatgaggtaa
     3901 ctaatacatg gaacccagag aaaaacgttc ttaaaaaagc ctattttttt gacatataca
     3961 ccaaattttg cgaagttatg gcttgactta tttttttatg agctttaaaa tagaaattat
     4021 ttcattggat ttttttataa aaaggaaaaa tataccttga ggaatatttt gaggaaaaac
     4081 gacatctcca gattttacat tttgctttaa ccaaataatt tgaccatcaa tggtcataat
     4141 ttctacaatg aataaccccg aggcaagcct caaggaattc ttttgattaa aattgataaa
     4201 aaatgacaga attaaaaatc aagtaaaaat gaattagatg tttacattgt ctatgatttg
     4261 aaaaagttcc tgaatagtat cagctcgaag catggcaatg cgtgtatttt taaaatttgg
     4321 aatattttta aaaataggtg tagctgccaa atggcagcga gcatgaataa ttccttttaa
     4381 acgaatattt gatattgttt caaaatttac ttcttcattc ttaaaatcaa tccattgaat
     4441 acaggaaaga atataatttt taagaatttc ccttttctgt tgaaatgata aagacgaaga
     4501 aatttcacct ttttccagaa attctttcat ttccttaaaa atccatggtt gtcctaaact
     4561 tcctctgcct atcatgatag catccactcc gtatttatca aaatattctt ttgctttttg
     4621 cggagtagtt acatctccgt ttccaataat aggaattttt atgcggggat tattttttac
     4681 ttccccaatc aaagtccaat ccgcttcgcc tctgtacatt tgcgaacgag tacgtgcatg
     4741 aatggttaat gcttgaatac cgcaatcctg caattgctct gctaaagtaa gaataatttt
     4801 gttgttttcg tcccagccaa gacgagtttt tacagttaca ggtaatttta ccgctttcac
     4861 tactgcctta gtaatttcca ataattttgg tatatctttc aacaatccag caccagcacc
     4921 tttatttgca acttttttca ctggacagcc aaaattcaag tcaataatat ctggttgtag
     4981 ctcttcagcc atcaaagctg cctgtaccat tgcgtccaca tgttggccgt agagttgaat
     5041 agctatcggt cgttcggact cgaaaaaagt aattttttga agtgatcgct caatttttct
     5101 gatcaaagcg tctgaagaaa caaattctgt ataaactaaa tcagcaccaa attgtttaca
     5161 caacaatcgg aaaggaggat cggttacttc ttccattgga gctaaaaaca aaggatagtt
     5221 tgggaaatca gtgttgccta ttttcattca ataacaggta aagaatttta atttttatct
     5281 gtacaaataa ttaaaaacaa ttgtttcaat tacaaaatta caagtttctg agggaataga
     5341 aaaaccctta tgtcaataga aaaatagcca atgaggaaaa acattggttc attaaaaaat
     5401 taaatgatta ttcgcaaagt atcctacttt aaaagagata aattacagta tatttttcaa
     5461 aaaatagtta atgagtaacc ccgaggcaag cctcgaggaa ttcttttgat taaatttata
     5521 aaaaatgtta attcttgtat ttttgatcat ttttgaaaat caaagtatat agttcatttc
     5581 gtctaataat tataaaacaa acattatatt tttcattaaa attttaaaca ttatgaaaaa
     5641 ggcagtattt ttattttttt cgttgttatt gataacagga tgggcatcct caaaaaatgt
     5701 agccattgat agtgtaaaac cagatgtgac ggtcacaaaa aatttaagta aagaagttaa
     5761 agattcattc ttgctccaaa aactttcgcc tcaacagttg attgaattga aaaaagcaga
     5821 attggaaaac gaaaggaaaa aaattaaagc tgaagcacga gaagatatgc cttttacagg
     5881 gtttcagtta ttcttaattt gtgttttacc ttttcttttt gtcattgtta tcatctggat
     5941 tatttactat cagaaaggaa aagaatcgaa acaaagatat gaattgtact taaaatcact
     6001 ggagcttggt caaaccttac ccgaagactt ttttgagaaa aaagccaaaa ataaacctaa
     6061 ctctgattta aagaaaggag ttttatggtt ggctgttggt ctatcgttgt tgatttattt
     6121 tctgattgta aaaaacatgg gagcactcat tttgggaatt gttccagctt ttgttgggat
     6181 aggttactta ctggtttatt ttttggagaa acctaaatcg ccagacgata ccgataaaga
     6241 tgaataatgc ggaagatctt cgatggattg ctcaagtcat tttatccaga gatgagagag
     6301 cctttgagaa attgatacgc aaatatcagt cacccattcg tcgattcttt ttgaacctga
     6361 caatgggtga tgtttttctc agtgacgatc tggctcagga aacttttatt aaagcttttc
     6421 tttatttgcc tacttttaag gctttatcga atttttcgac ctggcttttt cgtatcgctt
     6481 acaatgtgta ttatgatgcc gttcgagcta gaaaagattt ttatgatctg gacgagaaag
     6541 aaatcaatca gctatatcaa gtgaacaatt cctattcaac ttacaaagaa gatttttaca
     6601 aagctttaat caatttgaat gaaatggaaa aaatagccgt tttgctttat tatatggaag
     6661 acaaaacaca acgtgaaatt gcaactatta tgaaatgtcc tattggtagt gtaaaaaatt
     6721 atattcgaaa aggtaaagaa aaaattgcga attttttaaa agaaaatgga tatggaaacg
     6781 aatgaaacag acaaaaattt tcaggatttc tttaaactac acaagcatga tatagctgat
     6841 aatggtttcg ccagcagaac tttacggcat ttgccgcagc gaaaagacaa ttcgtggatc
     6901 gtttggcttt ttacaaccat tggaattgct acagctttgt atattggaat tttctctgac
     6961 tggttgatga actttataga acatatgttc aatttgccat tcatttatct gttaattggg
     7021 gttgcacttt tccccttggt aattggactt tgcattatgt tctgggaaaa agaacaatat
     7081 tgaaaagcga ttaataggtg gtaaaaagcg taagaaatta aatactgttt taaacaaaaa
     7141 agagagaaaa ttgactttct ctcttccaaa tttctttctt ta
//
LOCUS       DAOSAS010000093         7116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6754_length_7116_cov_12.798612, whole genome shotgun sequence.
ACCESSION   DAOSAS010000093 DAOSAS010000000
VERSION     DAOSAS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6754_length_7116_cov_12.798612"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..977)
                     /gene="hisF"
                     /locus_tag="PLR72_05350"
     CDS             complement(222..977)
                     /gene="hisF"
                     /locus_tag="PLR72_05350"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOK36659.1"
                     /translation="MLAKRIIPCLDVKDGKTVKGINFENIKNVGDPVELGAYYAQMGA
                     DELVFLDITATNEKRKTLSELVTRIAKEINIPFTVGGGISSIYDVSFLMACGADKISI
                     NTAAVLHPELITELAKNFGSQCIVLAIDTKFINGEWYVFLNGGRVPTDIKTIEWAKKA
                     VDLGAGEILLTSMDHDGTKNGFAIDITRAVSESVNVPVIASGGAGTMEHFVEVFKEGK
                     ADAALAASIFHYKEIEIPALKAYLKQKGIEVRL"
     gene            complement(1072..1836)
                     /gene="truA"
                     /locus_tag="PLR72_05355"
     CDS             complement(1072..1836)
                     /gene="truA"
                     /locus_tag="PLR72_05355"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOK36660.1"
                     /translation="MIVKRYFIYIAFKGTAYHGWQQQPNGISVQEVLTNAMNMILRSK
                     INLVGAGRTDTGVHAKSMVAHFDYEGTINEQSFLSHLNCVLPKDIAVFKLIEVKPTAH
                     ARFDAITRKYEYHVVTQKNVFKTDITLLINKKIDFQLMNEAAQLLKKYNDFTSFSKLH
                     THVKTNYCTIYQAEWQQQDEEWIFIVEANRFLRNMVRSIVGTLFEIGFKKINIEQFKE
                     IIEAKNRCKAGMSVPAKGLLLSEIKYPEKIFVVENN"
     gene            complement(1938..3698)
                     /locus_tag="PLR72_05360"
     CDS             complement(1938..3698)
                     /locus_tag="PLR72_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOK36661.1"
                     /translation="METTRLFDILDRYLEKYPQQEAALACKRNGEWKKFSIQEYVETT
                     NNISYGMLKLGIQPGDKVCIISNNRPEWNMIDFATMQIGALSVPIYPTISQDDYLYIL
                     NHAEIKMIFVEGKELRNKLMPIFPQIKTLQYIYTFVDQGVNLPYLDQLIELGKENPQP
                     EKLTELKNNIHSSDVATIVYTSGTTGVPKGVMLSHNNIVQQVLHLYDIPSPRDRIALS
                     FLPLCHAYERMLVYLYQYIGLSIYYAENLGTIAENIKEVNPQIMATVPRVLEKMYDKI
                     YLAGKKLPYFSKVIYYWALNLANSYQIENGWWKRFKLKIADKLVYSKWRENIGGDFDI
                     IVSGGSSLRPQLASFFSAIGMPVFEGYGLTETSPVIAVHQKVKNGRKFGTVGLPIPGV
                     EVKIGEQNEILCRGHNVMVGYYKDPELTAQVIDKDGWFHTGDTGKFTPEGQLIITGRL
                     KSLFKTSNGKYINPEIMETKFTESPFIENMIVVGENKKFAAALISPDFVFLRSWCEKH
                     KIPYTSDAEMVEHPEVIKRFQKEIQKYNVHFGDFEQIKRFQLVPDEWTPQNGFLSPNL
                     KIKRNVIEKYYADKIEKLFT"
     gene            complement(3711..4577)
                     /gene="purU"
                     /locus_tag="PLR72_05365"
     CDS             complement(3711..4577)
                     /gene="purU"
                     /locus_tag="PLR72_05365"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOK36662.1"
                     /translation="MKTKNDTAILLIYCPDKPGILASVTEFINRQQGNIIYLDQYVNR
                     EEKVFFMRIEWDISHFQIPKSKIEEYFSRLIALRFEMNFKLYFSDNKPRMVIFVSKLS
                     HCLYDLLARYASHEWNVEIPLIISNHRDLEPVARQFGIDFHFFPIDKSNKKEQESREL
                     ELLKNQNINFCVLARYMQVLSPKFIEKYPNKIINIHHSFLPAFAGAKPYHAAYERGVK
                     IIGATSHYVTQELDAGPIIEQDVARVSHKDSVDDLIRKGRDLEKIVLSRAVEKHIERK
                     ILTYKNRTIVFQ"
     gene            4783..6753
                     /locus_tag="PLR72_05370"
     CDS             4783..6753
                     /locus_tag="PLR72_05370"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOK36663.1"
                     /translation="MKKFIISFCTLFVTLLLFAQTSSLLNGTIIGTQLSVDYSTGNPS
                     TTVNTIQNVFDNNYETFFASYDRSGTWVGLDLGNKHVITKIGYSPRISQPDRVQLAVI
                     EGANSPDFSDGVPLYLIPEAAPENTMTYADIDCSRGFRYVRYVSPSDVRCNLAELQFY
                     GYPGEGTDTKFYQITNLPTVTIHTENSQDVVSKDTYIKGIVSFISENGNKIYTDSTSI
                     KGRGNASWNFPKKPYRLKLFNKVNLLGMPAKAKDWTLINNYGDKTLMRNILAFNVSKI
                     FNMPYTPAGICVDVILNGEYKGTYQLCDQVEVNKNRVNITEMKPTDTALPNLSGGYLI
                     EIDAYGPTTEKSWFYSSKGTPVTIHSPDDDEIVPEQTQYITNYFNNLEQEVYSDYTQD
                     NASFRKHLDTKTFLKNLLIGEFCGNTDTYWSTYMYKQRNNDTIYTGPIWDYDIAFEND
                     YRTYPINNLIDFIYRTNGSTAGDMQNFVDHVWASNASKRELYNLWMDARAKKLTLEMM
                     NHLVDSLATLLDQSQRLNFIRWPIMNSYVHMNPVVWGSYQNEVNNVKNYIVNRLTWMD
                     QKMTSIYPGPQAVENPFVEKWYVNHATLYLEGYDHPVSVKIFAIDGQLVTTKNLLASE
                     NFSTELYPGIYVIQVQDLITKAMSSQKVLITK"
BASE COUNT         2297 a         1221 c         1149 g         2449 t
ORIGIN      
        1 tttatgacaa atattggttt agcaaaacgt ttgattttat cagcggttaa aatcaagaaa
       61 ttttattttt attcgacgta gtctggagac tacgccgaac aagggatata aatagtagag
      121 ccgttgcatg caacggctct actaaagaat cagaattcaa tagatgtttc ctgaaatgtc
      181 tctactcgaa actatataaa ttttttttga aacaaaatgt attacaatct tacttcgatt
      241 cctttttgtt ttaaataagc tttaagagca ggaatttcta tttctttata gtgaaaaata
      301 ctggctgcca atgccgcatc agctttccct tctttaaaaa cttctacaaa atgttccatc
      361 gtacctgctc caccactggc aatgacgggg acattgacac tttctgaaac cgctctggta
      421 atatcaatag caaaaccatt tttggtccca tcgtgatcca tagaagtcag taaaatttct
      481 cctgcaccca gatcgacagc tttctttgcc cattctatag ttttgatgtc agttggaact
      541 ctgcctccat tcaaaaaaac ataccattct ccattgataa attttgtatc aatggctaaa
      601 actatacatt gactgccaaa attttttgcc agctcggtaa taagttccgg atgcaaaacc
      661 gctgctgtat ttattgagat tttatcggct ccacaagcca ttaaaaatga aacatcataa
      721 atacttgaaa tgcctcctcc tacagtaaaa ggtatattga tttctttagc aatccgtgtt
      781 accaactctg ataatgtttt ccttttttcg ttagtagcag taatatccaa aaaaaccagt
      841 tcatcagctc ccatttgtgc ataataagct cctaattcta cagggtcacc tacgttttta
      901 atgttttcga aatttattcc ttttaccgtt tttccatctt ttacatccag acaaggaatg
      961 attcgtttag ctaacatatt gatagacaat tatattttaa tttattctaa atgttgattt
     1021 tcctataaat tgaggaggta aaattaataa atttgaccca aataatgttg atcaattatt
     1081 ttccaccaca aaaatttttt ctggatattt gatttcagaa agcaataatc ctttggctgg
     1141 tacagacatt cctgctttgc aacgattttt tgcttcaatg atttctttaa attgttcaat
     1201 attaatcttt ttaaatccaa tttcaaacaa agtccctaca atgcttcgta ccatatttct
     1261 gaggaaacga ttggcttcta ctataaaaat ccattcctca tcttgttgct gccattctgc
     1321 ttgataaata gtacaataat tagtttttac atgggtgtgc aacttactga aacttgtaaa
     1381 atcattatat tttttcaaga gttgagctgc ttcattcatc aattgaaagt cgattttttt
     1441 atttattagt aaagttatat ctgttttgaa aacatttttt tgagtaacta cgtgatattc
     1501 atattttctg gtgatagcat caaaacgtgc atgggctgtt ggttttactt caatgagctt
     1561 gaagacggca atatcttttg gcaaaacaca atttaaatga gatagaaaac tttgttcatt
     1621 tatggttcct tcatagtcaa aatgagctac catcgatttt gcatgtacgc ctgtatctgt
     1681 ccttccagca ccaacaagat ttattttgct acgcaatatc atattcatag cgtttgtcaa
     1741 aacttcttgc acggaaatac cgtttggttg ttgttgccaa ccgtgatagg ctgtgccttt
     1801 aaaagctatg taaataaaat agcgtttcac aatcagagat aatctttgaa taattaaaaa
     1861 tctattttgg cgattttcta aaagaaaggt tataacgtta gtaatatttt tattttaact
     1921 taataaaatg ctgattttta agtgaataat ttttcaattt tatcagcata atatttttca
     1981 ataacgtttc tctttatttt cagattagga gataaaaatc cattttgagg tgtccattca
     2041 tctggtacta attgaaaacg ttttatttgt tcaaaatcac caaaatgaac attgtatttt
     2101 tgaatctctt tttgaaaacg ttttatcact tcaggatgtt caaccatttc tgcatcgctt
     2161 gtatagggga ttttatgttt ttcacaccag cttcttaaaa agacaaaatc aggagaaatt
     2221 aaagcagccg caaatttttt attttctcct accacaatca tgttttcaat aaaaggcgat
     2281 tcggtaaatt ttgtttccat aatttcagga ttgatgtatt tgccattcga agttttgaaa
     2341 aggcttttta atcttcctgt aataatcagt tgaccttctg gcgtaaattt tcctgtgtct
     2401 cctgtatgaa accaaccatc cttgtctatt acttgagctg ttagttctgg atctttgtaa
     2461 taacctacca ttacattgtg accccgacaa agaatttcgt tttgttctcc aattttgact
     2521 tctactccag gaatgggcaa gcctacggtt ccaaattttc ttccattttt tactttttga
     2581 tggaccgcaa taacagggga agtttcagtc aaaccatatc cttcgaaaac cggcattcca
     2641 atagcagaaa agaaagatgc cagttgtgga cgtaacgaag aaccaccaga aacaataata
     2701 tcaaaatcgc ctccaatatt ttctctccat tttgaataaa ctaatttgtc ggcaatttta
     2761 agtttaaaac gtttccacca cccattttct atttgatagc tattggcaag atttaaagcc
     2821 caataataaa tcactttgct gaaataaggc aattttttcc cagcaagata tattttatca
     2881 tacatttttt ccagaacgcg aggaacagta gccattatct gtggattcac ttccttaatg
     2941 ttttccgcta tagttccaag attttcagca taatatatcg ataaccctat atattggtat
     3001 aaatatacca gcattctttc gtaagcatga cataatggta agaaacttaa agcaattcta
     3061 tcgcgaggtg aaggaatatc ataaagatgc aaaacctgct gaacaatatt attatgtgac
     3121 agcattactc cctttggaac acctgtcgta cccgaagtat atacaattgt ggctacatca
     3181 gaagaatgaa tgttgttttt taattcagtg agtttttctg gttgtggatt ttcttttcct
     3241 aattcaatca actggtccag ataaggaaga ttaacacctt gatctacaaa agtataaatg
     3301 tactgtagtg ttttaatttg cgggaaaata ggcatcagtt tgtttcgcag ttcttttcct
     3361 tccacaaaaa tcatttttat ttcagcatga ttcagaatgt agagataatc atcctggcta
     3421 atagtagggt aaataggaac agaaagagcc ccaatttgca tagtggcaaa atcaatcata
     3481 ttccattctg gtcgattatt gctaataata caaactttat cacctggctg aataccaagt
     3541 tttagcattc catagctaat attgttagta gtttctacat attcctgaat gctgaatttt
     3601 ttccattctc catttcgttt gcaagccaaa gctgcttctt gttgaggata tttttccaga
     3661 taacgatcca gaatatcaaa taaacgagtt gtttccatga tattggtaat ttattgaaaa
     3721 acgatggttc tgtttttata agtaagaatt tttctttcaa tatgtttttc cactgctcga
     3781 gagagtacga ttttttcaag atctcttcct ttcctgatca agtcgtccac agaatctttg
     3841 tgagaaacgc gagcaacatc ctgttcaatg atcggacccg catcaagttc ctgcgtaacg
     3901 taatgactgg tagctccaat tatttttacc cctcgctcat aggcagcatg atacggtttt
     3961 gcaccggcaa aagcaggcaa aaaagaatga tgaatgttga tgattttgtt gggatatttt
     4021 tcgataaatt ttggagaaag aacttgcatg tagcgtgcca atacacaaaa attaatattt
     4081 tgatttttca acaattcaag ttctcgtgat tcttgttctt ttttatttga cttgtcaata
     4141 gggaaaaagt gaaaatcgat tccaaattgt ctggcaacag gctctagatc acgatgattg
     4201 ctgataatca atggaatttc tacattccat tcgtgtgatg catatcgagc aagcaaatca
     4261 tacaaacaat gagaaagttt ggaaacaaaa ataaccatgc gaggtttgtt gtccgaaaaa
     4321 taaagtttaa aattcatttc aaaacgcaag gcaatcaatc tactaaaata ttcttcaatt
     4381 ttgcttttag gtatctgaaa atggctgatg tcccattcaa tacgcataaa aaaaactttt
     4441 tcttctctgt ttacatattg atccagataa ataatatttc cttgttggcg attgataaat
     4501 tcggttactg atgccagaat tcctggttta tcagggcagt aaatcaataa gatggctgtg
     4561 tcgtttttgg ttttcattta aaatgaataa ttttaagaat gcaaagatat aactttttta
     4621 ttaattcaat ttttttaaat aagccacata tttgcttgac tttcttaaaa aataaatttt
     4681 taaaataaga aagtaatttc atgatacagt gagaaaaatg caaaagatga gaaaaattct
     4741 tttaactttg taacactatt gaaaacaagc aaaatgaatg ttatgaaaaa atttattatt
     4801 tctttctgca cgttatttgt tacattgcta ttgtttgcac aaacttccag cctgttgaat
     4861 ggaacaatta ttggcacgca actttcagtg gattattcta cggggaatcc ttcaacgacg
     4921 gtaaatacca ttcaaaacgt gttcgacaat aattacgaga cattttttgc ttcttatgac
     4981 cgaagtggta catgggttgg attggattta gggaacaagc atgtcattac taaaattggt
     5041 tattcaccac gtatctcaca acctgataga gtgcaactgg cagtgattga aggtgctaat
     5101 tctcctgatt tttcagatgg ggttccactt tatttgattc ccgaagctgc ccctgaaaac
     5161 actatgactt atgctgatat cgattgttcc cgtggatttc gttatgttcg ttatgtttct
     5221 ccttccgatg tacgttgcaa tctggctgaa ctacaatttt atggttatcc tggcgaaggc
     5281 acagatacaa aattttatca aattaccaat ctgccgacgg ttaccattca tacagaaaac
     5341 agtcaggacg ttgtttcaaa agatacgtat ataaaaggaa ttgtcagttt tatttctgaa
     5401 aatgggaata aaatttatac ggatagtact tctatcaaag gaagagggaa tgccagctgg
     5461 aattttccta aaaagcctta tcgattgaag ttgttcaata aagtaaattt actgggaatg
     5521 cctgctaaag caaaggactg gacgcttatc aataattatg gtgacaaaac ccttatgcga
     5581 aacatactgg cgtttaatgt cagcaaaatt tttaatatgc cctatacgcc agcaggaatt
     5641 tgtgtagatg tcattttaaa tggagaatac aaaggtactt atcaactctg tgatcaagta
     5701 gaagtaaaca aaaatagagt aaatatcacc gaaatgaaac ctactgatac tgctttgccc
     5761 aatctatcag gaggttattt gattgaaatt gatgcttatg gaccaactac tgaaaaatca
     5821 tggttttatt cttctaaagg cacacctgtt actattcatt ctcccgatga tgatgagatt
     5881 gtaccagaac aaacccaata tattaccaat tatttcaaca acctggaaca agaagtttat
     5941 agcgattata cacaagataa tgcttcattt aggaaacatc tagacaccaa aacttttctg
     6001 aaaaatttgc ttatcggtga attttgtggg aatacagata catactggag tacctacatg
     6061 tacaaacagc gaaacaatga tacgatttat acgggaccga tatgggatta tgatatagct
     6121 tttgaaaacg attatagaac ttatcctatc aataatttaa tcgattttat ttatcgaaca
     6181 aacggttcaa cagcaggcga tatgcaaaat tttgtcgatc atgtatgggc aagcaatgct
     6241 tccaaacgtg aactatataa cttgtggatg gatgccagag ctaaaaaatt gacactggaa
     6301 atgatgaatc atctggtgga tagtctggca acgctattgg atcaatcaca gagattaaat
     6361 tttattcgtt ggcccattat gaattcttat gttcacatga atcctgttgt atggggttcc
     6421 tatcaaaatg aagtgaacaa tgtaaaaaat tatatagtta atagattaac gtggatggat
     6481 caaaaaatga cttccatcta tccaggacct caagctgtag aaaatccttt tgtggaaaaa
     6541 tggtatgtca atcatgctac tttatatttg gaaggctatg atcatcctgt ttcagtaaag
     6601 atttttgcta tagatggtca gttagtaaca acaaaaaatt tattagcttc tgaaaatttt
     6661 tccactgaac tatatccggg aatttatgtt attcaggttc aagatttgat aacaaaagca
     6721 atgtccagcc agaaagtatt gataacgaaa taatttattg tttttaagaa attaatcgag
     6781 taggtagggg gattactccc ccgccctctc atactaccca gcatgctctc gaaccgggcg
     6841 gtttcattta atggtgtaac gcttcataat ttaatgaaag actgtgtcgg attttccttt
     6901 ccaaattttg taaaaatgaa ctaaaagcca atgtcaatca gtttttatga atattttagt
     6961 aaatttaatc tacgcaagaa aaatactatt ttttcttcta actaagttat tttattgaat
     7021 ttttttgtat gtttgtaaaa aataaaataa tgttcaatat atgataaaca actaataatt
     7081 acactgtaag caagaaatta ttttaaaaaa atatat
//
LOCUS       DAOSAS010000094         7078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6811_length_7078_cov_11.875979, whole genome shotgun sequence.
ACCESSION   DAOSAS010000094 DAOSAS010000000
VERSION     DAOSAS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6811_length_7078_cov_11.875979"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..512)
                     /gene="rplI"
                     /locus_tag="PLR72_05375"
     CDS             complement(69..512)
                     /gene="rplI"
                     /locus_tag="PLR72_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOK36664.1"
                     /translation="MEVILKEDISNLGYKGDIVKVKDGYARNYLIPTKKAVIATESAK
                     RMLEEDKKQRAHKLERIKNEALELAEKVKDITLVVGAKTSSKGKIFGSVGPIQLAEAF
                     EKAGFTIDRKLIELSEPVKEVGSYKLTLHLHREVTIEIPFEVISE"
     gene            complement(533..802)
                     /gene="rpsR"
                     /locus_tag="PLR72_05380"
     CDS             complement(533..802)
                     /gene="rpsR"
                     /locus_tag="PLR72_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOK36665.1"
                     /translation="MANNNEEIRYLNPPTVDIKKKKYCRFKKSGIKYIDYKDPEFLKK
                     FVNEQGKILPRRLTGTSLKYQRRVAQAIKRARHLALLPFVTDLLK"
     gene            complement(805..1155)
                     /gene="rpsF"
                     /locus_tag="PLR72_05385"
     CDS             complement(805..1155)
                     /gene="rpsF"
                     /locus_tag="PLR72_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOK36666.1"
                     /translation="MLNHYETVFILTPVLSETQMKETVEKFKSLLLENGAVITNEENW
                     GLRKLAYPIQKKTTGFYCLLQFDAEPNVITTLETNYRRDERVIRYLTVRLDKYAYEYA
                     EKRKNVKLKEGKEN"
     gene            complement(1492..4215)
                     /gene="ppdK"
                     /locus_tag="PLR72_05390"
     CDS             complement(1492..4215)
                     /gene="ppdK"
                     /locus_tag="PLR72_05390"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOK36667.1"
                     /translation="MSDKKRVYIFGNGQAEGNAQMKNLLGGKGANLAEMNLIGIPVPP
                     GFTITTEVCSEYYQLGKEKIVEILKPEVEKAMEWIEKLMKSKLGDPENPLLVSVRSGA
                     RASMPGMMDTILNLGLNDEIVEGLSKKTGNPRFAWDSYRRFIQMYGDVVLGMKPENKN
                     DVDPFEKIIEDLKKERGIKLDTELTVDDLKELVARFKKAVRERTGKEFPESVYDQLWG
                     AICAVFDSWMNERAILYRKMEGIPDDWGTAVTVQAMVFGNMGDTSATGVAFTRNAATG
                     ENVFNGEYLVNAQGEDVVAGIRTPLQITKAGSQQWAKLAGISEEERRTKYPSMEEALP
                     DIYKELNEIQQKLENHYKDMQDIEFTVQEGKLWLLQTRSGKRTGTAMVKIAIDMLHEG
                     IIDEKTALLRIDPLKLDELLHPIFDKESLKNVKVIAKGLPASPGAASGKIVFNADDAT
                     EWAKQGEKVIMVRIETSPEDLAGMAAAQGILTARGGMTSHAAVVARGMGKCCVSGAGA
                     LKIDYVNRTIQINDLVLKEGDYISINGSTGEVYLGKVPTKEPELDPYFIELLTLADKY
                     ADLRVRTNADTPRDASVARKFGATGIGLCRTEHMFFEGNKIKAMREMILAEDTEGRKK
                     ALAKLLPYQRADFEGIFEVMEGLPVTIRLLDPPLHEFVPHDEKGQQEMAEAMGVPLKK
                     IQERVESLREQNPMLGHRGCRLGNTFPEITEMQTRAILEAVLNLKAKGIKIIPEIMVP
                     LVSVKKELVEQERIIRETANKIFEEKGDTIEYKVGTMIEVPRAALTANEIAENADFFS
                     FGTNDLTQMTFGFSRDDIGSFLPIYLEKKIIESDPFQILDQTGVGQLVKMGVEKGRTT
                     KPDLKIGICGEHGGEPSSIEFCHRVGMDYVSCSPYRVPIARVAAAQAAIKN"
     gene            4533..>7078
                     /gene="clpB"
                     /locus_tag="PLR72_05395"
     CDS             4533..>7078
                     /gene="clpB"
                     /locus_tag="PLR72_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOK36668.1"
                     /translation="MNFDNFTIKSQEAIQDAIELVQSKGQQAIETPHLLKAILRTGED
                     VVQYLFGKLGVNTNALNLALDKMIDAYPRVSGAEPYLSRETNAVLQKAIDITSKSGDQ
                     FVSLESILLALVTEKNAVTRMLNDAGVNERTLSEAINTLRKGRKVTSETAEETYNALN
                     KYAINLNEQARAGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGTGKTAIVEGLA
                     QRIVRGDVPENLKTKQIFSLDMGALIAGAKYKGEFEERLKAVINEVVNSNGDVILFID
                     EIHTLVGAGKSEGAMDAANILKPALARGELRAIGATTLDEYQKYFEKDRALERRFQIV
                     MVDEPDVASSISILRGLKERYENHHKVRIKDDALIAAVELSHRYITDRFLPDKAIDLI
                     DEAAAKLRMEIDSVPEEIDVIERNIKQLEIEREAIKRENDEKKLEQLNQEIANLKEKS
                     QALRAKWSSEKALIDKIQQAKIEIEDLKFQAERAEREGDYGKVAEIRYGKIKALEDAI
                     AKYQEELAKIQGSNPMIKEEVDSEDIAEIVSKWTGIPVTRMLQSEKEKLLHLEEELHK
                     RVVGQDDAIQAVADAIRRSRAGLQDPKRPIGSFIFLGTTGVGKTELAKALAEYLFNDE
                     NMMTRIDMSEYQEKFSVTRLIGSPPGYVGYEEGGQLTEAIRHKPYSVVLFDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRIVNFKNTIIIMTSNIGSSLIRENFEKLNEYNHDEIIE
                     KTRNEVFELLKQTIRPEFLNRIDEIIMFKPLNRPQIEEIVRLQLKNIQEMLKQNGIQL
                     EATDKAIKLIADLGYDPQFGARPVKRVIQKLILNDLSKQIIAGAIQSNQTV"
BASE COUNT         2270 a         1277 c         1187 g         2344 t
ORIGIN      
        1 caaataaaaa accggtaact cttttaagtt atcggttttt ttatttgctc tctttttaaa
       61 tatctgactt attcagaaat tacttcaaaa ggaatttcta ttgtaacttc tctatgcaag
      121 tgaagtgtca atttatagga accaacctcc ttaacaggtt cgcttaattc tatcaatttg
      181 cggtcgatag taaaaccagc cttttcaaaa gcttctgcca gttgaatagg tccaaccgaa
      241 ccaaaaattt ttcctttaga actggttttt gcacctacta ccaatgtaat atcttttact
      301 ttttctgcca attcaagtgc ttcgttttta atacgttcca atttgtgtgc acgctgtttc
      361 ttatcttctt ctagcattcg ttttgcactt tcggtagcaa tcacggcttt tttggttgga
      421 atcaggtaat tccttgcata accatctttt actttcacaa tatcaccttt ataaccaagg
      481 ttgctgatat cttctttcaa tatcacttcc ataatttttt accctccagt ttttatttca
      541 ataaatcagt tacaaaagga agtaaagcca gatgacgagc tctctttatg gcttgggcta
      601 ctctgcgttg atattttaac gaagtacctg ttaaacgacg tgggagaatt tttccttgct
      661 cgtttacaaa ttttttcaag aattcaggat ctttataatc gatatattta ataccacttt
      721 ttttgaagcg acaatatttt ttctttttaa tatcaacggt aggaggattt aagtatctta
      781 tttcttcgtt gttgtttgcc atttttaatt ctcctttcct tctttaagtt taacattttt
      841 ccttttttca gcatattcat aagcatattt gtccaaacgg acagtaagat aacgtatcac
      901 acgttcatca cgacgataat tggtttccaa tgttgttatt acatttggtt ctgcatcgaa
      961 ctgcaaaagg caataaaaac ctgtcgtctt tttctggatg ggataagcta attttcgcaa
     1021 tccccaattt tcctcattgg taattacagc accattttca agcaaaaggg acttgaattt
     1081 ttctaccgtt tccttcatct gagtctcaga caaaacggga gttaaaatga aaacggtttc
     1141 ataatgattt aacatactaa ttattaacgt tttaaatttg acatttttct taaaagcgtt
     1201 tgcaaagtta taaataatta tttgattgac aaaaggattc aacagctttt ttaattctat
     1261 tatagaactg tcattagaaa caagttttat gacttaaatg aaaagtatat aaaatattga
     1321 aaatcatcac aaatgttttt ttagtatatg acaaaaagat gatattttct tttttttaag
     1381 tatttatccg agtaacgatg tgtttggctg aaattttttt tatcaatgat aattctcggt
     1441 ttaccaaaaa gggcagctca attgaactac cctttttgta aatatctatt cttagttttt
     1501 tatggcagct tgagctgcag ccacccgagc aataggaact ctataaggag aacaacttac
     1561 ataatccatt cccacacgat gacaaaattc tatagaagaa ggttcgccac catgttcacc
     1621 gcaaatacca attttcaaat ctggttttgt agtccgacct ttttcaacac ccatttttac
     1681 taattgccct acgcccgttt gatcaagtat ctggaaagga tccgactcaa taattttttt
     1741 ctctaaataa attggtaaaa aagaaccaat atcatctcgt gaaaacccaa atgtcatctg
     1801 agtaaggtca ttggtaccaa atgagaaaaa gtcagcattt tcagctattt cattagcagt
     1861 taatgctgct cggggaactt ctatcatagt tcctacttta tattcaatag tatctccttt
     1921 ctcttcaaat atcttatttg ctgtttcacg aataattctt tcctgctcaa ctaactcttt
     1981 ttttacactt actaatggga ccatgatctc tggaataatt ttgattcctt ttgctttcag
     2041 attcaagaca gcttcgagaa tagcacgtgt ttgcatttca gtaatttctg ggaaagtatt
     2101 tcctaacctg catccacgat gacctaacat tggattttgt tcacgcaatg attctacgcg
     2161 ttcttgtatt ttctttaaag gaacacccat tgcttcagcc atttcctgtt gacctttttc
     2221 atcgtgggga acaaattcat gtaggggagg atctaaaaga cggatagtaa caggcagtcc
     2281 ctccataact tcaaaaattc cttcaaaatc agctctttga taaggaagaa gttttgctaa
     2341 agcttttttt ctaccttcag tatcttccgc caaaatcatt tctcgcatgg ctttaatctt
     2401 atttccttca aagaacatgt gttcagttct acataaacca atacccgtag caccaaattt
     2461 tcttgccaca gaagcatcac gcggagtgtc tgcattggtt ctaactctta gatcagcgta
     2521 tttgtcagct aatgtcaata attcaatgaa gtagggatct aactcaggtt ctttggtagg
     2581 aacttttccg agataaactt ctcctgtgga tccattgata gaaatatagt caccttcttt
     2641 taaaactaaa tcattgattt gaatagttct gttgacataa tcaattttca acgctccagc
     2701 tcctgataca cagcacttcc ccattccacg agctacgact gcagcatgag aagtcattcc
     2761 cccgcgtgca gttaatattc cctgagctgc tgccatacct gccaaatctt caggtgaagt
     2821 ttcaatgcgt accatgataa ctttttcacc ttgttttgcc cattcagtag catcatcggc
     2881 attgaaaaca atttttccac tggcagctcc cggtgaagct ggcaaacctt tagctataac
     2941 tttgacattt ttcaacgatt ctttatcgaa aattggatgc agtaattcat ctaattttaa
     3001 aggatcaatt cttaacaaag ctgttttttc atctataatg ccctcgtgaa gcatgtcaat
     3061 ggcaatttta accatagccg ttcctgtcct tttaccgctt cttgtttgca aaagccaaag
     3121 tttaccttct tgaacagtaa actcaatatc ttgcatatct ttataatgat tttcaagctt
     3181 ttgttgaatt tcatttagtt ctttataaat atccggtaaa gcttcttcca tagaaggata
     3241 ttttgttctt ctttcttctt ctgaaattcc agcaagtttt gcccattgtt gtgaacctgc
     3301 ttttgtaatt tgaagaggag tacgaatacc agctactaca tcttcacctt gtgcattaac
     3361 taaatattct ccattaaaaa cattttcgcc tgtagcagca tttcgtgtaa aagctacacc
     3421 tgtagcagaa gtatcaccca tattaccaaa aaccattgcc tgaacagtaa cagcagtacc
     3481 ccaatcgtca gggatacctt ccattttccg atataaaatg gcacgttcat tcatccagga
     3541 atcaaataca gcacaaatag cgccccacaa ttggtcataa actgactctg gaaattcttt
     3601 tcctgtccgt tctcgaacag cttttttaaa tcttgctacc agttctttca aatcatcaac
     3661 agtaagttca gtatctaatt ttattcctct ttctttcttt aaatcttcaa ttattttttc
     3721 aaatggatcg acgtcatttt tattttcagg cttcattccc aagaccacat caccatacat
     3781 ttgaataaaa cgacgatagg aatcccatgc aaagcgtgga tttcctgttt ttttacttaa
     3841 tccttctaca atttcgtcat tcaatcctaa atttaaaatc gtatccatca ttcctggcat
     3901 ggaagcacgc gccccagacc gtaccgaaac gagcaaggga ttttcaggat ctcccaattt
     3961 ggatttcata agcttttcaa tccattccat tgctttttcc acttctggtt tcaaaatttc
     4021 tacaattttt tcttttccta attgatagta ttcagaacaa acctcagtag taatagtaaa
     4081 acccggaggc acaggaattc caatcaaatt catttcggct aaattagctc ctttcccacc
     4141 taataaattt ttcatttggg cattgccttc tgcttgtcca ttgccgaaaa tataaactct
     4201 ttttttatcg ctcatattaa aaccttgttt ataagtaatt ataattttgt tattttcatt
     4261 ctgcaaaaat aatttttttt atcagaaaaa aaaagaatta caaaaattgt catctccaag
     4321 ttacaatttc aactttatat taagggaata atttttactg aaatattgtc agtgcttctt
     4381 tatactattt cgacaaaaaa gacattctta ccgaaatatt taattggtaa ataatttgta
     4441 gagaataaaa taaaatttta aatttatttt tcaattttta ttaataaaag acaatttaaa
     4501 aagattttat tttaatataa aaaattaaag ttatgaattt tgataatttt acaattaaat
     4561 ctcaggaagc cattcaagat gctattgaat tggtacagag caagggtcaa caagctattg
     4621 aaactccaca cttgttaaag gcaattctcc gaacgggaga agatgttgtc caatatttat
     4681 ttgggaaatt aggagtgaat acaaatgcct tgaatttagc cttagataaa atgatcgatg
     4741 cttacccaag agtgtcaggt gctgaaccat atttaagtcg tgagacaaat gcagtactac
     4801 aaaaggcaat agatattact tccaaatctg gtgatcagtt tgtgtctctg gaatctattt
     4861 tattggcact cgtaactgaa aaaaatgcag ttacgcgaat gttgaatgat gcaggagtga
     4921 atgaaagaac gctttctgaa gccatcaata ctttgcgaaa aggaaggaaa gtaacttctg
     4981 aaacagcaga agaaacttat aatgcattaa ataaatatgc aattaatttg aacgaacagg
     5041 cacgtgcagg aaaacttgat cctgttattg gcagggatga agagattcga cgagttttac
     5101 agattttgag tcgtcgtaca aaaaataatc caattttaat tggcgaacca ggaacaggaa
     5161 aaacggctat tgttgaaggc ttggctcaaa gaattgtaag aggtgatgtg cctgaaaatt
     5221 tgaaaacaaa acaaatattt tcactggata tgggtgcctt gattgctgga gctaaatata
     5281 agggtgaatt tgaagaacgg ttgaaagccg ttatcaatga agtagtaaat tccaatggtg
     5341 atgttatttt gtttattgat gaaatccata cgttggttgg ggcaggcaaa agtgaaggtg
     5401 caatggatgc tgccaatatt ttaaaacctg ctttagctcg aggagagctg cgagctattg
     5461 gtgctactac attagatgaa tatcaaaaat attttgaaaa agacagagcg ttagaacgtc
     5521 gtttccaaat agtaatggtt gacgaacctg acgtggcaag ttcaatttct attttacgtg
     5581 gtttgaaaga aagatatgaa aatcatcata aagttcgaat aaaagatgat gctctaatag
     5641 ctgctgttga actttcgcat cgttacatta ctgatcgttt tttgcctgat aaagccattg
     5701 acttaattga tgaagccgct gcaaaattaa gaatggaaat tgattctgtt cctgaagaga
     5761 ttgatgttat tgaaagaaat ataaaacaac tggaaattga acgagaggct attaaaaggg
     5821 aaaatgatga aaagaaatta gaacaattga atcaggaaat tgctaatttg aaagagaaaa
     5881 gtcaagcttt acgagcaaaa tggtcaagcg aaaaagcttt gattgataaa attcaacaag
     5941 ccaaaatcga gatagaagat ctgaaatttc aggcagaacg tgctgaaaga gaaggagatt
     6001 atggtaaagt agctgaaatc cgttacggaa aaatcaaagc tctggaagat gcaatagcta
     6061 agtaccaaga ggaacttgca aagatacaag gctcgaaccc aatgataaaa gaggaggttg
     6121 acagcgaaga cattgctgaa attgtaagca aatggacggg catacctgtg acaagaatgc
     6181 ttcaaagtga aaaagaaaag ttattacatt tggaagaaga actccataaa cgagttgttg
     6241 gtcaagatga tgccattcag gcagtagcag atgccatccg tcgcagtaga gccggtctgc
     6301 aggatcctaa acgtcccatt ggctcattta tttttcttgg tacgactggc gttggaaaaa
     6361 ccgaattggc aaaagctttg gctgaatact tgttcaacga tgaaaacatg atgacccgaa
     6421 ttgacatgag tgaatatcag gaaaaattct ctgttacacg attaataggt tcgcctcctg
     6481 gctatgtggg ttacgaagaa gggggacaat tgacagaagc aattcgtcat aaaccttatt
     6541 ccgtggtact ttttgatgaa attgaaaaag ctcatcctga tgtatttaat attttacttc
     6601 aagtattgga tgatgggaga ttgactgaca ataagggaag aattgtgaat tttaaaaata
     6661 ctattatcat catgacctct aacattggat cctctttaat tcgagaaaat tttgagaaat
     6721 taaatgaata taatcatgat gaaattattg aaaaaacaag aaatgaagta tttgaattat
     6781 taaaacaaac cattcgtcct gaatttttga accggattga tgaaatcatt atgtttaaac
     6841 cgctgaatcg gcctcaaatt gaagaaatcg tacggttgca actgaagaat attcaggaaa
     6901 tgctcaaaca aaatggtatt caattagaag ccacagacaa ggcaattaaa ttgattgctg
     6961 atttaggtta cgatccacag tttggagcac gtcctgttaa acgagtcatt caaaagctga
     7021 ttcttaatga tttgtcgaaa caaattattg caggtgcaat tcaaagtaat cagacagt
//
LOCUS       DAOSAS010000095         7061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6837_length_7061_cov_10.693548, whole genome shotgun sequence.
ACCESSION   DAOSAS010000095 DAOSAS010000000
VERSION     DAOSAS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6837_length_7061_cov_10.693548"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..1045)
                     /locus_tag="PLR72_05400"
     CDS             complement(335..1045)
                     /locus_tag="PLR72_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOK36669.1"
                     /translation="MASLYLIPTPLGTTSLEKIFPPYNIEIINRLNHFVVEDVRTVRR
                     FLKQISQERNINEVKFYLLNEHSSKEELVSLLEPLYNGFDLGLMSEAGCPAVSDPGAD
                     LVALAHKNNFTVIPLIGPSSVLLSLMASGFNGQNFEFVGYLPVQSEERIKALRRLEAK
                     VYAENQTQIFIETPYRNMKMLETILKVCQPSTKLCIAADITLESEFIKTKSIGEWRKN
                     LPDLQNKPCIFLLYKTWG"
     gene            complement(1368..2204)
                     /gene="pyrF"
                     /locus_tag="PLR72_05405"
     CDS             complement(1368..2204)
                     /gene="pyrF"
                     /locus_tag="PLR72_05405"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOK36670.1"
                     /translation="MTKHELFEQIKKKKSFLCIGLDTDIHKIPSHLLKSSEDPIFDFN
                     RAIIDATAKFCVAYKPNLAFYESAGTKGWESLARTVNYLKNNYSEQFLIADAKRGDIG
                     NTSQKYAEAFFENLNFDAITLHPYMGKDTVVPFLNYKNKWIILLILTSNKSAADFQLL
                     QLSGNGDYLFEKILKDSMNWGNEENIMYVVGATQAEMLKKVRAIAPHHFLLIPGIGVQ
                     GGNLEEVASAGLNEGCGLLVNSSRQIIYASSEKDFASAAQVAAKKLQTTMEYLLKQHG
                     II"
     gene            complement(2242..3327)
                     /gene="prfA"
                     /locus_tag="PLR72_05410"
     CDS             complement(2242..3327)
                     /gene="prfA"
                     /locus_tag="PLR72_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOK36671.1"
                     /translation="MANNSLLEKLEGLQNKFEEVSILINDPAVLGDQKKFTKLNKEYH
                     DLEQILSVKKEYEIALTNLAEAKKILENETDADLREIAKMEIEQLEKLIPDLEEKIKL
                     LLIPADPADKKNAIVEIRSGTGGDEAALFAGDLFHMYAKYCENKGWKIEITDFNEGTA
                     GGYKQIVFTIRGENVYGTLKYESGVHRVQRVPQTETQGRIHTSAATVAVFPEAEEFDI
                     ELKESDIRVDIFCSSGPGGQSVNTTYSAIRLTHIPTGITVQCQDEKSQHKNKLKAMTE
                     LRSRLYAIEYQKYLDEISSKRKTMVSTGDRSAKIRTYNFPQGRVTDHRINYTLYNLPA
                     FMNGEIQPLIDQLIIAENTERLKAAEL"
     gene            complement(3569..4153)
                     /locus_tag="PLR72_05415"
     CDS             complement(3569..4153)
                     /locus_tag="PLR72_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOK36672.1"
                     /translation="MPQKVSKTKKLLIEVARQLFSEKGKKNVTMNDIAEASKKGRRTL
                     YIYFKSKDEIYKAVIDNELDYIFEQIAVVSTENIPPDSKLTKHIITHLDAIKKSVIRN
                     GSLKADFFRDIYEVERARRKIDMKEIALIKTILVEGIEKNCFKKMNVELSSIIILYAL
                     KGLEVPYIRQNISNEFEKNKYSIVEFVLTGIKKK"
     gene            complement(4388..5350)
                     /locus_tag="PLR72_05420"
     CDS             complement(4388..5350)
                     /locus_tag="PLR72_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOK36673.1"
                     /translation="MNPILYRLYNSFLLALPKAFKTIIWLLKIILPISFVVSLLQYWG
                     IIAIVASFLAPVFSIVGLPGEAAIVFITSIFMALYAPIAIIATLPLGIREITILAIMC
                     LISHNLIVETTVQKKTGSSPWIIFFLRIIASFVAAFLLNKILPDNMGKAQIVEKAIIF
                     KSFGEMFQHWLINTGWLILKIVLIITGLMILQNILKEFHILEYLSKIFAPLMKIMGLS
                     QNTTFLWFVAQIVGLTYGSAIMIEEVTNNSITVEDANLLNYHIAVNHSLLEDTLLFVA
                     IGVPAGWIIVPRFILAILIVWSFRGIHSFESYLKQKQAKFADFS"
     gene            complement(5871..6581)
                     /locus_tag="PLR72_05425"
     CDS             complement(5871..6581)
                     /locus_tag="PLR72_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOK36674.1"
                     /translation="MILNCWIIDDEPLAINLLESYVEKTPFLKLTGKYSSALAAMHDL
                     ENQQPNIIFLDIQMPELSGMEFSQFVHPEARIIFTTAFSEYAINGYKVNALDYLLKPI
                     SFTDFLTAAKKALNWFEISQKAAQNEGTEKTGIFVKADYKLVQVLFDDILFVEGLKDY
                     VKIYVRNDTKPIITLMSMKGMEDLLPRDRFIRVHRSYIVQKNKIENISKNRIMITGHK
                     ILIGETYRQNLMDEIGNI"
     gene            complement(6553..>7061)
                     /locus_tag="PLR72_05430"
     CDS             complement(6553..>7061)
                     /locus_tag="PLR72_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOK36675.1"
                     /translation="VQDLSKLLRYILYDNNQTFVPLSQEVEFMQNYINLMRIRLPENV
                     ELITDFELLPDSNINIAPLIFISLIENAFKHGISADKPSFIHIQLIENTEGLLQFTCE
                     NSYFPKSESDKSGKGIGLQQLQKRLDLLYPNRYIWKKEIINNVYSTLLIINTNNEPED
                     DTQLLDHR"
BASE COUNT         2399 a         1163 c         1080 g         2419 t
ORIGIN      
        1 gtataaaaca atagttttca gtggaattta tgctggcatt cagaatttta aaggtttgtt
       61 aacagaaacg aatgcagatg gcgttttttc ctatgaaggc ataacgacca tgaagcaaat
      121 cggtataaga ttaggtcttc aattttaaaa aaggtgcagt tagtcaaaaa atggggaaaa
      181 attctaattg cgttgattat caatgacatt aataatctaa caattaaatt ataacttcac
      241 aagttataat tttctcaggg attaatacca gattatataa tttttttgaa aagtcaaatc
      301 aattaactct ttgaaatgca ctttaatttt ctgtttaacc ccaagttttg tataataaaa
      361 aaatgcacgg tttattttga agatcaggca agtttttccg ccattctcca atgctttttg
      421 ttttaataaa ttcactttct aaagtaatgt ccgctgcaat acataattta gtagaaggtt
      481 ggcatacttt taaaatagtt tccaacattt tcatgtttct gtaaggagtt tcaataaaaa
      541 tttgtgtttg attttcagcg tatacttttg cttcaagtcg acgaagtgct ttgattcgtt
      601 cctcagattg gacaggtaaa taaccgacaa attcaaaatt ttgtccattg aaccccgaag
      661 ccatcaacga aagcaaaaca gaagatggac caatcaaagg gatcacggta aaattgtttt
      721 tatgtgccaa agcaactaaa tcggcgccag gatctgacac cgctggacac cctgcttcag
      781 acatgagtcc caaatcaaaa ccattgtaca gtggttctaa taaggaaact aactcttctt
      841 ttgaagaatg ttcatttagc aaataaaatt tcacttcatt gatatttctt tcctgagaaa
      901 tttgtttcaa aaatcttcta acggttcgca catcttctac cacaaaatgg ttcaatcggt
      961 taataatttc tatattgtaa ggaggaaata ttttttctaa cgaggtcgtt cctaacggtg
     1021 tgggaatgag atacaaactt gccataactt ttttgaaaaa tgtgaacaat aaaggagcaa
     1081 aactaaagaa ttaataaaga taaaaaatga ttttatacca aattgttatc attaatttta
     1141 atttgaatag tcataacaga atattaacat caaaccgatg tgaaattgaa tgcgtttttc
     1201 aaaaggatgc ttcgcttcaa aactatttcc tttatgctct gcagattact gtctatgcaa
     1261 atccaaaggc aaaaaggtgg cacaaattgt aaatttgagt cagtagtaag tactttcttt
     1321 ttattcaaca ataaataaaa tttttaagct taacaataat aaaaacatta aattattcca
     1381 tgttgtttaa gcaagtattc catagtagtt tgcaactttt ttgctgctac ctgagcagca
     1441 ctggcaaaat ctttttcaga agaagcataa ataatctgtc gtgaagaatt taccaacaat
     1501 ccacaacctt catttaaacc agccgatgcc acttcttcca aatttccacc ttgtacacca
     1561 attccaggaa ttaataagaa atgatgaggg gcaatagctc gtactttttt caacatttca
     1621 gcttgtgtag ctccaacaac atacatgata ttttcttcat ttccccaatt catcgaatct
     1681 ttcaggattt tttcaaacaa ataatcacca ttacccgata attgaagaag ttgaaaatct
     1741 gctgcacttt tgttggaagt caaaattaat aaaataatcc atttgttttt ataattcaaa
     1801 aaaggaacaa ctgtatcttt tcccatgtaa ggatgtaagg tgatagcatc aaaattcaaa
     1861 ttttcaaaaa aggcttctgc atatttttga gatgtgtttc caatatctcc tcgcttggca
     1921 tcggcaataa gaaactgttc tgaataattg tttttcaaat aatttactgt tcgggctaaa
     1981 gattcccaac cttttgttcc agcactctca tagaatgcta agtttggttt ataagccaca
     2041 caaaactttg ctgtagcatc aataatggct cggttaaaat caaaaattgg atcttctgat
     2101 gattttaata gatgagaagg tattttgtga atatcagtat caagtccaat gcataaaaat
     2161 gattttttct tttttatctg ctcaaaaagt tcatgcttag tcataaaatt atttttaaaa
     2221 tcatggtaat gaaaaaaata attataattc agcagctttt aatctttcag tattttcggc
     2281 aatgataagt tgatcaatca gcggttgaat ttcaccattc ataaaagctg gaagattata
     2341 aagtgtataa ttaatccgat ggtcggtaac tcgtccttgt gggaaattat aggtacgaat
     2401 ttttgcagaa cgatcaccag tagataccat tgttttacgt ttcgaactaa tttcgtccag
     2461 atacttttga tattcaatag cgtacaagcg tgaacgtaat tctgtcattg ctttcaattt
     2521 atttttatgc tgggattttt cgtcttgaca ttgaacggta ataccagttg gaatatgtgt
     2581 caaacgaata gcagaatagg ttgtattgac cgactgtcca cctgggccgg aggaacaaaa
     2641 aatatctaca cggatatccg actctttcaa ttcaatatcg aattcttcag cttcaggaaa
     2701 caccgccaca gtagctgctg aagtatgaat tcgtccttga gtttccgttt gtggaacacg
     2761 ttgcacgcga tgaacacctg actcatactt aagcgttcca tacacatttt cgccccttat
     2821 agtaaaaaca atttgtttgt aaccacccgc tgttccttca ttaaaatcgg taatttctat
     2881 tttccaacct ttgttttcac aatattttgc atacatatga aacaaatcac cagcaaaaag
     2941 agcagcttca tcccctccag tcccactccg tatttctaca atggcatttt tcttatctgc
     3001 tggatcggca ggaatgagaa gcaattttat tttttcttcc aaatcaggaa ttaatttttc
     3061 taactgttca atttccattt tagctatttc acgtaagtcg gcatcagttt cgttttcaag
     3121 tatttttttt gcttcagcca aattagttaa agctatttca tattcttttt ttacggaaag
     3181 aatctgctct aagtcatggt attccttatt aagttttgtg aatttttttt gatcccctaa
     3241 caccgcagga tcatttatta aaattgaaac ttcctcaaat ttattttgta gtccttccag
     3301 tttttccaat aaagaattat tagccatctt cttgtttttt acaaattacc tcttcttttc
     3361 aactgcaaaa ttataacaaa actttaattt tcagaaatga ttgtcgtctg tttagatgaa
     3421 taacaactct tgtcttttca agcagtgttg aattctaact ttcattgagc actcaataat
     3481 ctataactga ttttgaatga ttaattacac ttttcgaaag gtaataaatt gatagccaaa
     3541 gataaaataa aaaagctgta tgattgggtt attttttctt tatgccagtt agtacaaatt
     3601 caacgatact atatttattt ttttcaaatt cattgctgat attttgtcgg atataaggaa
     3661 cttctaatcc ttttaaagca taaagaataa taatagagga gagttctaca ttcatttttt
     3721 taaaacaatt tttttctatt ccttctacaa gaattgtctt aattaaagcg atttccttca
     3781 tgtcaatttt tcgtcgggca cgttctacct cataaatatc tctaaaaaaa tcagctttta
     3841 gcgagccatt tcggattacc gattttttaa tggcatcaag atgagtaata atatgtttgg
     3901 taagcttact atcaggagga atattttctg tagagactac agcaatttgc tcaaaaatat
     3961 agtctaattc attgtcaata actgctttgt atatttcatc cttgctctta aaataaatat
     4021 ataaagttcg tcttcctttt ttagaagctt cggcaatatc attcatcgtg acattctttt
     4081 tccctttttc ggaaaaaagt tgccttgcca cttcgatcaa taattttttt gttttagaaa
     4141 ctttttgagg cataatttat ttcttgatag aataagcaaa gttaaaaaat aaaacttcac
     4201 ttgttacttt gaaaaaaata ttttcctaac ttttattcag aaagataaaa taatccttgt
     4261 gacgaaaatt tctgtgatct ataataagaa agataatatt cgcaccgaga acaaaaattg
     4321 ataggacaaa ttgcaaattt gtacaagtat ggcaaaaaat aagacaagag gaaatttaat
     4381 gtccctttta agagaaatca gcaaattttg cctgtttttg tttaagataa ctttcaaagc
     4441 tatgaatacc tcgaaaactc caaacaataa gaatagctaa aataaatcga ggtacgataa
     4501 tccaaccagc aggcacacca attgctacaa acaataacgt gtcttcgagc aaagaatggt
     4561 ttactgcaat atgatagttc aataaattgg catcctctac agtaatagag ttatttgtta
     4621 cttcttcaat catgatggct gaaccataag taagtcctac aatttgagca acaaaccaca
     4681 aaaaagtagt gttttgagaa agacccataa ttttcattag aggagcgaaa atttttgata
     4741 gatattccag aatatgaaat tctttcaaga tattttgcaa aatcattagt cctgtaataa
     4801 ttaggactat ttttaaaatc agccagcctg tattaatcaa ccaatgttga aacatttctc
     4861 caaaactttt aaatataatt gctttttcta cgatttgagc tttgcccata ttatcaggca
     4921 aaattttatt caaaagaaaa gctgccacaa acgaagcaat gattcgaaga aaaaaaatga
     4981 tccatggaga agagcctgtt ttcttctgaa cagtagtttc gacaatcaag ttatgcgaaa
     5041 tgaggcacat aatagcaaga atagtaattt cacgtattcc caaaggcaaa gttgcaataa
     5101 tggcaatagg agcataaaga gccataaaaa tactggtaat gaaaacgata gcagcttctc
     5161 ccggtaaacc aacaattgaa aaaacaggag ctaaaaaaga agcaacaatg gctataattc
     5221 cccaatattg aagtaaactc acaacaaaac tgataggtaa aataattttt aatagccaaa
     5281 ttatggtctt aaatgcttta ggaagggcta agagaaagga attatataag cgatataaaa
     5341 taggattcat tagaaaagta cttgttctat aaaacaagaa ggggttgtct cacgacaacc
     5401 caatcttttt taattaacct taaatctaat accatgaaaa acatatgcaa atgtagttat
     5461 attttgtttt gccacaaagc attaaatact attttttttg tatttagaaa taattaacct
     5521 attttttata aaatcgtgat cagataagaa aaacttaagg aatatttaaa aatatctatc
     5581 attccctctt atattatttg acataattat acttgaacag cttttgaaat taaattaata
     5641 ttttactttt ttctaagatt ctattgtaga tattatcttt tgaataattt tacgataaaa
     5701 agtgtttaca aaaagccatt agtaataaaa aaaattattg gacttttcta aaaaataaaa
     5761 tagagcgtta ttcatcaatt tttaagagag gttgaaacca attattaaat tcatataaag
     5821 caatgaataa ccccgaggca agcctcgagg aattcttttg attaaaaaaa tcaaatattc
     5881 cctatttcgt ccattaaatt ctgtcggtag gtttcgccga taagaatttt atgtccggta
     5941 atcatgatcc ggtttttgct gatattttct attttgtttt tctgaacaat ataggagcgg
     6001 tgaacgcgaa taaatctgtc gcgtggaagc aaatcctcca tgcctttcat gctcattagc
     6061 gtaatgatgg gtttggtatc gtttctgaca tagattttca cataatcttt taatccttcc
     6121 acgaaaagga tatcgtcgaa aagcacttgc accaacttgt aatcggcttt tacaaaaatt
     6181 cccgtttttt ctgttccttc gttttgagca gctttttggg aaatttcaaa ccagttcaag
     6241 gcttttttgg cagccgtaag aaagtcggta aatgaaatcg gtttaagcaa ataatcaagg
     6301 gcgtttacct tgtaaccgtt aatggcatat tcactgaaag cggtagtaaa aataattctt
     6361 gcttcgggat gaacaaactg tgaaaattcc atgccgctga gttcgggcat ttgaatatcc
     6421 agaaaaataa tgttaggttg ctgattttcc aaatcgtgca tagccgctaa ggcactcgaa
     6481 tatttgcctg tcagtttcag aaaaggtgtt ttttctacat acgattccag cagattgatg
     6541 gcaagtggtt cgtcatcgat gatccagcag ttgagtatca tcttcaggtt cattattggt
     6601 gttgataatc aaaagagtag aatagacatt gttaataatt tcctttttcc agatataacg
     6661 attcggataa agaaggtcta atcttttttg taattgctgc aatcctatgc ctttaccact
     6721 tttgtctgat tcagacttgg ggaaataact gttttcacag gtaaattgca ataatccttc
     6781 tgtgttttca attaattgaa tatgaataaa cgaaggtttg tcagcactga ttccatgttt
     6841 gaaagcattt tctatcaaag aaataaaaat taaaggagca atgttaatat tactatctgg
     6901 taataattca aagtcagtaa tcaattcaac attttctggc agccttattc gcattaaatt
     6961 gatgtagttt tgcataaatt caacttcttg tgacaaaggt acaaaagttt gattgttatc
     7021 atacagaata tatcttaata atttacttaa atcttgtact g
//
LOCUS       DAOSAS010000096         7031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6891_length_7031_cov_12.992689, whole genome shotgun sequence.
ACCESSION   DAOSAS010000096 DAOSAS010000000
VERSION     DAOSAS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6891_length_7031_cov_12.992689"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1355)
                     /locus_tag="PLR72_05435"
     CDS             complement(12..1355)
                     /locus_tag="PLR72_05435"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOK36676.1"
                     /translation="MDNIKLSIDNAFVTSEKIIEYQERVNECMAALHNRTGKGSDFLG
                     WLHLPSTIEDTLLKDIEDTAKNLRERCEIVVIIGIGGSYLGAKAVIDALSNSFEWLQP
                     KYKSPLIVFAGQNICEDYLYELQELLKNKKFGIICISKSGTTTEPALAFRLLKTQLEE
                     QQGKEEAQKLIIAITDKSKGALRKLAEQEGYKTFVIPDDVGGRFSVLTPVGLLPIAVA
                     GFNIRQLIAGAAQMEKICDIDVPFGENPATHYAVVRNELYKMGKKIEILVNYHPKLHY
                     ISEWWKQLYGESEGKENKGIFPAAVDFTTDLHSMGQWIQEGERIIFETVISIKEPNYL
                     KIFPYDKDNLDGLNFLAGKRIDEVNKMAELGTLIAHVDGGVPNIKIEIPKLNEYYLGE
                     LIYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEEQTKAIQARLKK"
     gene            complement(1425..2420)
                     /locus_tag="PLR72_05440"
     CDS             complement(1425..2420)
                     /locus_tag="PLR72_05440"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOK36677.1"
                     /translation="MNVKGKIAVIGGGSWATAIAKIVLTNEDTINWYMRRQEQIDEFI
                     RLSKNPSYLTGVKFDTERINFTNDINKVVRSSDLLIFATPSPFLKQHLKKLRVKLNKK
                     FIVSAIKGIVPDENLIVTEYFQQFYGILPENLAVLAGPCHAEEIALERLSYLTIACTS
                     REKARILANRLATNFVKTSISDDVIGIEYASVMKNIYAVAAGICHGLKYGDNFQAVLL
                     SNAIHEMSRFCNAMNPIHRDINESAYLGDLLVTAYSKFSRNRLFGTMIGKGYSVKTAQ
                     MEMEMIAEGYYAVKCIHEINENYQINMPIVDAVYQILYNKSSPILEIRKLTEELI"
     gene            complement(2501..3781)
                     /gene="glyA"
                     /locus_tag="PLR72_05445"
     CDS             complement(2501..3781)
                     /gene="glyA"
                     /locus_tag="PLR72_05445"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOK36678.1"
                     /translation="MKRDQIIFDVIEREKQRQLKGIELIASENFVSDQVLQAMGSVLT
                     NKYAEGYPGKRYYGGCEIIDESEQLAIERLKNLFDAEWVNVQPHSGSQANQAVYFACL
                     KPGDKILGLNLSHGGHLTHGSPVNISGILYQPLEYNVNVETGRVDYDQMEEVALRERP
                     KLIIGGASAYSREWDYKRMREIADKIGALLMIDMAHPAGLIATGLLQNPLKWAHIVTS
                     TTHKTLRGPRGGIILMGKDFPNPWGKSTPKGEIKMMSSLLDSAVFPGVQGGPLEHVIA
                     AKAVAFEEALQPEFKQYQLQVKKNAETLANELISRGFSIVSGGTDNHCMLVDLRSKYP
                     DLTGKIAENTLVEADITVNKNMVPFDSRTPFQTSGIRLGTPAITTRGAKEDLMVEIAE
                     MIETVLDNIDNKKIILSVKERVNKTMNNYPLFAY"
     gene            complement(3896..4471)
                     /locus_tag="PLR72_05450"
     CDS             complement(3896..4471)
                     /locus_tag="PLR72_05450"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOK36679.1"
                     /translation="MSKISWKPVNMVYPLPAALISCGNSPEEYNLMTVSWVGTICSNP
                     PMCYISVRPERHSHSIIKKNKEFVINLTNEELAYATDWCGVKSGKDYNKFKEMNLTPL
                     KGEKVNAPIVEESPLNIECKVKEIISLGSHDMFISEVVNIQVDNKYFDLKTHSFHLEK
                     AKLIANLNSHYYKLGEEIGKIGWTIKEKKRK"
     gene            complement(4538..4993)
                     /gene="pyrI"
                     /locus_tag="PLR72_05455"
     CDS             complement(4538..4993)
                     /gene="pyrI"
                     /locus_tag="PLR72_05455"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOK36680.1"
                     /translation="MEKELKVAALRNGTVIDHIPVDKLYKVVFMLHLDRCENQITIGN
                     NLDSVKMGKKGIIKIAGKFFAEDETNKIALIAPNANINIIKDAQVIEKRALSLPNEIH
                     DIIQCNNPNCITNHQNVPTFFYVLKEAEGTFLKCHYCERIISQEEVKIK"
     gene            complement(5083..5988)
                     /gene="pyrB"
                     /locus_tag="PLR72_05460"
     CDS             complement(5083..5988)
                     /gene="pyrB"
                     /locus_tag="PLR72_05460"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOK36681.1"
                     /translation="MSIKSLVSITDYSKEDILEILSSAEEFEKHPNQEVLKGKVIATL
                     FFEPSTRTRLSFETAVVRLGGSVIGFSDVATSSLSKGESLHDTIRIVSNYVDAIVMRH
                     PYEGAARYAAEISPVPIINAGDGANQHPSQTLLDLYSILKTQGTLENLNICIVGDLKY
                     GRTVHSLIMAMSHFHPTFTFIAPEELRIPYEFKIFCDKNNISYTELYELSDNFNEADI
                     LYMTRVQKERFQDLMEYERVKNVYMLKNSMLENTKPNLRILHPLPRVFEIDPEVDKNE
                     KAYYFQQAKNGLYVRQAIINKVLSS"
     gene            complement(6328..6930)
                     /locus_tag="PLR72_05465"
     CDS             complement(6328..6930)
                     /locus_tag="PLR72_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOK36682.1"
                     /translation="MLSTYKTIANKSEGIYKDKGSKFFSFAVPTQSVDGVKELVKNFK
                     EEFHDARHICYAYVIGIEHKEWRANDDGEPPGTAGRPILGQINSRGLTNILVVVVRYF
                     GGILLGTSGLATAYREAAADALNHAEIVEKTIDNFFTIRFNYSKINEVIRLLKEFNGE
                     IFNKNYDKNEYIIEFSIPKKDAALLISKLKNITGLTIEQQ"
BASE COUNT         2266 a         1305 c          976 g         2484 t
ORIGIN      
        1 tttagaaatg cttatttttt taatcttgct tgaatagctt tggtttgttc ttcatagcca
       61 ggtttcccaa gcaaagcaaa catatttttc ttgtatgctt ctacaccagg ctgatcgaaa
      121 ggattgacac ccaaaagata gccactaatg ccacaagcct tttcaaagaa ataaatcaac
      181 tcacccaaat aatattcatt caatttcgga atctcaattt taatattagg aactcctcca
      241 tccacatgag caatcaaagt gcctaattca gccattttgt ttacttcatc gattcttttg
      301 ccagcaagaa aattcaaacc atctaaatta tctttgtcat aaggaaaaat ttttagataa
      361 ttaggttcct taattgaaat tactgtttcg aagataattc gttctccttc ttgtatccac
      421 tgacccattg aatgtaaatc ggtagtaaaa tcaactgctg cagggaaaat acccttattt
      481 tcctttcctt cgctttcacc ataaagttgt ttccaccact cagaaatata atgtaatttc
      541 ggatgataat taaccagaat ttcaatcttt ttacccattt tataaagttc atttcttaca
      601 acggcatagt gagtagcagg attttctcca aaaggaacat ctatatcgca aattttctcc
      661 atttgagcag cccctgctat tagttgtcgt atattgaagc ccgcaacagc tattgggagt
      721 aaacctacag gagtaagaac tgaaaatcta cctccaacat catcaggaat gacaaatgtt
      781 ttataacctt cttgttctgc aagtttgcga agtgcacctt ttgatttgtc ggtaatagca
      841 ataatcaatt tttgtgcttc ttcttttcct tgctgttctt ctaattgagt ttttaaaaga
      901 cgaaatgcca aagcaggttc agtggtggtg cctgattttg aaatgcaaat aatgccaaat
      961 tttttattct tgagaagttc ctgaagttca taaagataat cttcacaaat attttgtcct
     1021 gcaaaaacaa tcaatggtga tttatatttt ggttgcaacc attcaaaact gtttgaaagt
     1081 gcatcaataa cggcttttgc tcctaaataa cttcctccaa taccaataat cactactatt
     1141 tcgcaacgtt ctcttaaatt ttttgctgta tcttcaatgt cttttaacaa tgtatcttca
     1201 atagttgaag gtaaatgtaa ccaaccaaga aaatcgcttc cttttcctgt acgattatgc
     1261 aaagctgcca tacattcatt aacacgttct tgatactcta ttattttttc tgaggtgaca
     1321 aatgcattgt caattgaaag tttgatatta tccattttct attttatttt ttgaaatgta
     1381 ttagtaatta aaaaaataat ttgaaaattt acctttaatt tttttcaaat taattcctca
     1441 gtaagtttcc gtatttcaag aataggcgaa cttttattat ataaaatctg gtaaaccgca
     1501 tccacaatag gcatattgat ctgataattt tcattgattt cgtgaataca tttcacggcg
     1561 tagtaacctt ctgcaatcat ttccatttcc atttgagcag ttttaacaga atatcccttc
     1621 ccaatcatag taccaaataa gcgatttctg ctaaacttgg aataagccgt taccagtaaa
     1681 tcaccaagat aagccgattc attaatatcg cgatgaatag gattcatagc attgcagaaa
     1741 cgactcattt catgaatggc atttgaaagc aacaccgctt gaaaattatc accgtatttt
     1801 aatccatgac aaatacctgc ggctactgca taaatatttt tcataacgga agcatattca
     1861 atgcctatta catcatcact aatagaagtt ttaacaaaat ttgtagccaa acgattggca
     1921 agtattctgg ctttttcacg cgaagtacaa gcaatagtca gataagataa tctttccaga
     1981 gcaatttctt cagcatgaca tggacctgct aatacagcta aattttcagg taaaattcca
     2041 taaaactgct gaaaatattc tgtaacaatc agattttcat cgggtacaat tcctttaatt
     2101 gctgaaacaa taaatttttt atttaattta acccttaatt ttttcaaatg ttgtttcaag
     2161 aaaggtgaag gtgtagcaaa aatgagcaga tccgaagaac gcaccacttt gttaatatca
     2221 ttcgtaaaat taattcgttc tgtatcaaac ttaacccctg tgagataaga tggatttttg
     2281 ctcaaacgaa taaattcatc aatttgttcc tgacgacgca tgtaccaatt aattgtgtct
     2341 tcatttgtaa ggacaatttt tgcaatggca gttgcccagc tcccgccacc aattactgca
     2401 attttccctt ttacattcat cgttgaatca tgctaaatat taatctttca tctcgccaaa
     2461 tcattgtctt ttctctgtaa aaaaagactt aaatcatttc ttaataagca aacaatggat
     2521 aattgttcat agttttattg acacgttctt ttaccgacaa aataattttc ttattgtcaa
     2581 tattatccaa tactgtttct atcatttcgg caatttcaac cattaaatct tccttagctc
     2641 cacgagtagt aatagctggg gttcccagtc gaattcctga agtctgaaaa ggagtacggg
     2701 aatcaaaagg caccatattt ttattgacgg taatgtcagc ttctaccaga gtgttttctg
     2761 ctattttccc agttaaatca ggatattttg agcgtaaatc taccagcata caatgattat
     2821 cagttccacc agaaacgata gaaaatcctc gtgatataag ttcattagcc aatgtttctg
     2881 cattcttttt tacttgaagt tgatattgtt tgaattcagg ttgaagggct tcttcaaaag
     2941 caacggcttt cgcagcaata acatgttcca gtggaccgcc ctgaacaccg ggaaaaacag
     3001 ccgaatccag caaagaagac atcattttta tctcaccttt tggtgtagac ttgccccaag
     3061 gattgggaaa atcttttccc atcaaaataa ttcccccacg aggacctctc aaagttttat
     3121 gagtggtgga agtgacaatg tgtgcccatt ttaaaggatt ttgaagtaaa ccagttgcaa
     3181 ttaagcccgc tggatgagcc atatctatca ttaacaaagc tccaatttta tctgcaattt
     3241 ctctcatgcg tttgtaatcc cattcacgtg aataagcgga agcaccacca ataattaatt
     3301 ttgggcgttc acgcaaagca acttcttcca tctggtcata atcaactctc cctgtttcta
     3361 cattcacatt gtactcaagt ggttgatata gaatccctga aatatttact ggggaaccat
     3421 gcgtcaaatg tccgccgtgt gacaaattta atcctaaaat tttatcacct ggcttcaaac
     3481 aggcaaaata aactgcctga ttggcttgtg aacctgaatg aggctgcaca tttacccatt
     3541 ctgcatcaaa aaggttcttt aaacgttcaa tagcaagttg ttcactctca tctattattt
     3601 cgcaaccacc gtaatatcgt ttgcccggat aaccttctgc atacttatta gtcaaaacag
     3661 agcccattgc ctgcaatact tgatcgctca caaaattttc agaagcaatc aattcaattc
     3721 cttttaactg tcgttgcttt tctcgttcaa taacatcaaa tattatttgg tctcttttca
     3781 ttataatttt ctttttttct tattattaac aatttcaaaa acaattttta taaccaattt
     3841 atttacaaaa tagaattact tttatttgtt tattttttat ttgtttgata aataattatt
     3901 tacgtttctt ttctttgatt gtccaaccaa tttttccaat ttcttctcct aatttatagt
     3961 aatgactatt tagattggca attaattttg ccttttccaa atgaaaagaa tgagttttta
     4021 aatcaaaata tttattatcg acctgaatat taactacttc ggaaataaac atatcatgag
     4081 aacctaaaga aataatttct tttaccttgc attcaatatt taaaggcgat tcttcaacaa
     4141 tgggagcatt tactttttca cctttcaaag gagtaaggtt catttcttta aatttattgt
     4201 aatcttttcc actctttaca ccgcaccaat cggtagcata agcaagctct tcattagtaa
     4261 gattaataac aaattcttta ttttttttaa tgattgaatg ggagtgccgt tcaggacgaa
     4321 cagaaatata acacattggc ggatttgaac aaatagttcc tacccagctt accgtcatta
     4381 aattatattc ttcaggacta tttccacacg aaatgagagc cgctggtaaa ggatatacca
     4441 tgttgacagg tttccaactt atttttgaca tatgattcaa ttaaataatt aaattaatta
     4501 aaaattagca atctaaacat ttcttatgct tcaaaattta ttttattttg acttcttctt
     4561 gagaaattat cctttcacaa tagtgacatt taagaaaagt gccttctgct tctttcaaaa
     4621 cataaaaaaa ggtaggtaca ttttgatgat tggtgataca attaggatta ttgcactgga
     4681 taatatcatg aatttcattc ggtaaactta gagctctttt ttcaattact tgagcatctt
     4741 taataatatt aatattggca ttcggagcta tcagagcaat tttattggtc tcatcctcag
     4801 caaaaaattt tccagcaatt ttgataatcc cctttttccc cattttaacg ctatccaagt
     4861 tattcccaat agtgatttga ttttcacacc tatccaggtg taacataaaa actactttat
     4921 ataatttatc taccggtata tgatctataa cggtaccatt tcgcaaagct gcaactttta
     4981 actctttttc cataacatat taatcaaagt aaacaatata taattgagaa gagagataaa
     5041 aaatttttct aattttatat taaaatcgta atcaattcat tattaagaag aaagcacttt
     5101 attaataata gcctgccgaa catacaatcc attttttgcc tgctgaaaat aataagcttt
     5161 ttcattttta tctacctctg gatcaatttc aaaaacgcga ggcagtggat gaagaatgcg
     5221 taaatttggt tttgtatttt caagcattga atttttcaac atatatacat tttttacccg
     5281 ctcatattcc attaaatctt gaaaacgttc tttttgaact cgtgtcatgt ataaaatgtc
     5341 tgcttcatta aaattatcgc ttaattcata taattcggta taagaaatat tatttttatc
     5401 acaaaaaatc ttaaattcat aaggtattcg caattcttct ggtgcaataa aagtaaatgt
     5461 tggatgaaaa tgcgacattg ccattatcag cgaatgaact gttcttccat attttaaatc
     5521 gcctactata caaatattta aattttctag tgttccttgt gtttttaaaa ttgaatacag
     5581 gtcgagcaat gtttgtgaag gatgttgatt ggctccgtcg ccagcattga tgattggaac
     5641 aggagaaatt tctgcagcat aacgtgccgc accctcataa ggatgacgca tcacaattgc
     5701 atcgacgtaa ttgcttacaa tgcgaatagt atcatgtaat gactcacctt ttgataagct
     5761 actggttgcc acatcagaaa agccgatgac agaacctccc aaccgtacta ctgctgtttc
     5821 aaaactaagt cttgtcctgg tggaaggttc aaaaaacaaa gtggcaatta ctttcccttt
     5881 caaaacttct tgattgggat gtttctcaaa ctcttctgcc gaggatagga tttccagaat
     5941 gtcttccttg ctataatcgg ttatggaaac taaactttta atgctcatta ttgcaagtcg
     6001 attttttaat atttaccaat tctcaaacct tactatagaa acatttaaaa catgaaaata
     6061 aaaaaaccaa tatatatctt gtacgaatta tatattggtt ttcaaaattt taactgctaa
     6121 ttttcttaac aagctacaaa aaataaaaaa atattgaaaa tttgttcttt tcattccctt
     6181 atattgatca ataaatcttc tcaaaaaaga aatatttgtt ttgcaaatat attgattttt
     6241 atttaaaaaa actgtttttt tgttttaaaa acccgaacaa aaatttataa aataactcaa
     6301 aatcttttat taatcgatat tgaaaattta ttgctgttca atggttaatc ctgtaatatt
     6361 cttcagtttt gaaattaaaa gagccgcatc ttttttagga atggaaaatt caattatata
     6421 ttcattttta tcgtaatttt tattgaaaat ttccccatta aattctttta agagtcgtat
     6481 cacttcattt attttagaat aattaaaccg aatggtaaag aaattatcaa tggttttttc
     6541 aacaatttca gcatgattca aggcatcagc agccgcttcc cgatatgcag ttgccaagcc
     6601 acttgttccc aataatatcc caccaaaata acgaaccacc actactaata tattagtcaa
     6661 tcctcgtgaa ttgatctgcc ctaaaatagg tcgtccagca gttccaggag gttctccatc
     6721 atcattggct ctccattctt tatgttcaat accaatcaca taagcataac aaatgtgacg
     6781 agcatcatga aattcttcct tgaaattttt taccagttcc ttaactccat ccacgctttg
     6841 agtgggaact gcaaaggaaa agaatttgct ccctttatcc ttataaattc cttctgattt
     6901 attagcaata gttttatagg tacttagcat tttgcgaatt gaatttttta atacttctaa
     6961 ctatgagaac ttcgctgcaa agatatttat ttattttatt tcgtctaatt ttctttgata
     7021 gaaaaatagt a
//
LOCUS       DAOSAS010000097         6987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_6951_length_6987_cov_14.022649, whole genome shotgun sequence.
ACCESSION   DAOSAS010000097 DAOSAS010000000
VERSION     DAOSAS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6951_length_6987_cov_14.022649"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLR72_05470"
     CDS             <1..233
                     /locus_tag="PLR72_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36683.1"
                     /translation="KNLLFAKKHIIFTFDFEKIIFCERAVLFLNTNLKNNIIIIQKGS
                     FLSGYSPERRNKGVQWKMKRNQDAPMCSLERG"
     gene            complement(409..2247)
                     /locus_tag="PLR72_05475"
     CDS             complement(409..2247)
                     /locus_tag="PLR72_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOK36684.1"
                     /translation="MTNKKRKRTYILYFFGIYFFISFFSSCANKAQGPTGGPKDTIPP
                     QIIRSVPQNGALNFAKKQILVDFDENISVEKIAENVIISPPQRKPPEIKAIGKRLVVN
                     LLDTLKPNTTYTIDFGNAIVDLNEKNPLKNYVFSFSTGNEIDSLKIAGTVINAEDLNP
                     ISGIIVGIYDELSDSVFLQKPFLRIGRTDEEGDFSINNVKKNTYRIYALGDVNRDYHF
                     QPGEGLAFTDSLIIPDFRKEEVQDTIWKDSVTIDTIRTVIRTHFLPDDILLRYFKESK
                     KRQYLVKYERKQPYMFTLYFNTSLAEMPHIKPLNFDWEGKHLVQKNNTNDTITYWLTD
                     SICWEKDTLQIEMTYLKTDSLYQLIPTTDTLNIFMRKAISASKSKAQKSPIAALFKQD
                     YKFTTNLTSTFEIYLPIQIRFNSPLANVDLSKIQLSEQKDSLFKPLPVQWHQVDSTQM
                     FYAIDYKWEPQKTYKFEIDSAAFTSIYHQVNKKYSTTFKIRSLDEYASLKIVLAEYHP
                     NAIIQLLDEKDKVLYTKAAQASGTVFEYLKPTSYYVRMFLDENGNGKWDTGDFSQHRQ
                     PEEVFYLPKKMTLIPNWEFEEVWDYKAVPLLKQKPKEIRKDASKQK"
     gene            complement(2307..>2651)
                     /locus_tag="PLR72_05480"
     CDS             complement(2307..>2651)
                     /locus_tag="PLR72_05480"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOK36685.1"
                     /translation="WQTHYLFGESSGKRTKKLGTASINSILINTIVPFLFCYGLKKSN
                     EDLKEKSLQILEQLPSEKNALIKGWKNAGLESETAFDSQALIQLKRNYCEEKKCLFCR
                     IGHKVLTLQMTE"
     assembly_gap    2652..2751
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2752..3150)
                     /locus_tag="PLR72_05485"
     CDS             complement(<2752..3150)
                     /locus_tag="PLR72_05485"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOK36686.1"
                     /translation="MKEAILQYVWKHKLFYQNQLYTVDGEKVEIIDVGKYNVDGGPDF
                     FNSKIKIGNTLWAGNVEIHSQSSDWLKHQHQFDKAYDSVILHVVGEANGEVFRTDGSK
                     IPQLVLTFPPKIETNYETLLNNQQWIPCADK"
     gene            complement(3208..3813)
                     /locus_tag="PLR72_05490"
     CDS             complement(3208..3813)
                     /locus_tag="PLR72_05490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOK36687.1"
                     /translation="MNLQFPKLLRPFIGKYTWRKLPSSNTIYITFDDGPVPEVTPFIL
                     KLLDEYGIKATFFCVGENVKKYPELYQEVLSRGHQVGNHTYNHLKGCSVSKEEYLNNI
                     EKAARLIDSNLFRPPYGKMKRSQQKALNEKYELILWDVITYDYNTKLSPQKVFNNVKH
                     YTRNGSIILFHDSIKARNNVLAVLPLAIEFWNKKGYNFGVI"
     gene            complement(3814..>6987)
                     /locus_tag="PLR72_05495"
     CDS             complement(3814..>6987)
                     /locus_tag="PLR72_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOK36688.1"
                     /translation="AFAIAFVTYLTTLEPTASFWDCGEFIATAYKLEVGHPPGAPFFM
                     MTARLFSLFTSDPSKVAVMINTLSALCSALTILFLFWTITHLAKKILIKSEENYTLAN
                     IIIIIGAGLVGALAYAFSDTFWFSAVEAEVYAYSSLFTAVVFWLILKWEEKADEPGSD
                     RWLLLIAYLMGLSIGVHLLNLLAIPAIVIVYYYKKYTPSTKGTFLALLVSVVLLAVVL
                     YGVIPGSVQVACWFELFFVNTLGLPFNSGLYVYLILTIAALAWAIYESYVAKNYFRMT
                     FSVLLAITLAGIPFFGSHIVLGIILIAALGIYFYHGKSKVSPHFLHTSILMITLMLIG
                     YSTYALIVIRSSANPPLDENSPDNVFALKYYLNREQYGDRPLFYGATYNAPVKLRIEG
                     NMCIPERKVGEPMYTPKPKTTPDEKDIYLKTGNKFDYKMDERFCMFFPRMYSSSPNHI
                     QAYKEWGKVKGKQITYDYCGQQKTDVCPTFFENLRFFFNYQLNFMYWRYFLWNFSGRQ
                     NDLQGNGEVDKGNWITGINFIDNMLVGNQKNLPTALATNKGHNTYYMLPLLLGILGIF
                     SQIYGGKEGRRGFWTVLLLFFMTGIAIVVYLNQTPYQPRERDYAYAGSFYAFSIWIGL
                     GLIELFKTVNKYLSDISSSILVTLLSLGVPTLMAAQNWDDHDRSERYTARDFGHNYLA
                     SCRQNAIIFTNGDNDTFPLWYNQEVEGNRTDVRCCNLSYLQTDWYIDQMKRQAYESDP
                     LPISWKKEDYLSGKNEVVQVEDVINQPLQLKTVIDFILSTDSYTKIDGEPFIPTKKVY
                     LPIDPEEVLKTGTLKPERANEIIPQIDISLKNRLTKSELMVLEMLKENKWERPMYFAV
                     TVGDDYYLGLTDHFELTGLAYQILPIGVKGAGPGVNVDEMYDNVMNKFRFGGVNNPKV
                     YLDENIVRMCKTHRMFITQLAGALIAKNDSVRAEKVLDYCNEMIPGTSVRHDYSSLMM
                     GQYYYQLQQPAKGDKILEQIANDCVEYLNWSLQLSPTQQNSITSEMGNNMAILNEVLR
                     IFDEAERKDLANKYFPYYVQYSKHFRM"
BASE COUNT         2277 a         1207 c         1074 g         2329 t
ORIGIN      
        1 taaaaaattt gctttttgca aaaaaacata ttatctttac atttgatttt gaaaagataa
       61 ttttctgcga gagagcagta ttatttttaa atactaattt aaaaaataat attataatta
      121 tacaaaaggg ttcctttctt tctggttact caccggaaag aaggaacaag ggtgttcaat
      181 ggaagatgaa gcgtaaccaa gatgcgccga tgtgtagctt ggagagaggt tgactcatgc
      241 ggttcatata aaacttctaa tgaacacccc ctttttttgt atatctttta aacttttctt
      301 caaaaaattt ttagtagaat aagaaagaag atagaaatta ttttttcaaa aatctgtatt
      361 tgaaagaaat tcaagttatg ttctcttctt ttaaattatc agggtatttt atttttgctt
      421 cgacgcatct tttcttattt cttttggttt ttgtttcaaa agcggaacag ccttataatc
      481 ccaaacttct tcaaattccc aattaggaat cagcgtcatt tttttaggca aataaaagac
      541 ttcctctggc tgacgatgct gagaaaaatc acctgtatcc catttcccat ttccattttc
      601 gtctaaaaac attcgtacat aataacttgt aggctttaga tattcaaaaa cagttcctga
      661 ggcttgtgcg gctttcgtgt ataatacctt gtccttttcg tccaggagct ggataatggc
      721 attgggatga tattcagcca aaacaatttt tagagaagca tattcatcca aagaacgaat
      781 tttgaaagtt gtagaatatt ttttgttgac ctgatgataa atgctggtaa aagccgctga
      841 atcaatttca aatttatatg ttttttgagg ttcccatttg taatcaatgg catagaacat
      901 ttgggtagaa tctacctggt gccactgaac aggcaaaggc ttgaataaag aatctttttg
      961 ttcacttaat tggattttag ataaatctac atttgctaaa ggggaattaa aacgaatctg
     1021 aataggaaga taaatttcaa aagtactcgt tagattggtc gtaaatttat aatcctgttt
     1081 aaataaagct gcaataggtg atttttgagc ttttgatttt gaggcactta tagcttttct
     1141 cataaaaata tttaaagtat cggtagttgg aatcaattga tacaacgaat cggtttttaa
     1201 ataggtcatt tctatttgta aagtatcttt ctcccaacat attgaatctg ttaaccaata
     1261 agttatggtg tcattggtgt tgtttttttg aactaaatgt ttcccctccc aatcaaaatt
     1321 aagcggtttt atgtggggca tttctgcaag cgaagtattg aagtacaaag tgaacatgta
     1381 aggttgtttt cgctcatact ttaccagata ttgtcgtttt ttgctttctt taaaatatcg
     1441 caaaagtata tcatcgggta aaaaatgagt tcgaattaca gtccgaattg tgtcaatggt
     1501 taccgaatct ttccagatgg tatcttgcac ttcttctttt ctaaagtccg gaataattaa
     1561 tgaatcggta aatgccaatc cttctcctgg ttgaaaatgg taatctcggt taacatctcc
     1621 taaggcataa attcgataag tgtttttttt cacattatta attgaaaaat ctccttcttc
     1681 gtcagttcga ccaatacgaa gaaacggttt ttgaagaaaa acagaatcgg acaattcatc
     1741 ataaatacct acaatgatgc ctgaaatagg attcagatct tctgcattaa taactgttcc
     1801 tgcaattttt aaagaatcaa tttcattgcc agttgaaaat gaaaatacat aattttttaa
     1861 aggatttttt tcatttaaat caacaatggc attaccaaaa tcaatagtgt aagtagtgtt
     1921 aggttttaaa gtatctaata aattaacaac caatcttttc ccaatagctt taatttcagg
     1981 aggtttgcgt tgaggaggtg aaataattac gttttcagca attttttcaa cactgatatt
     2041 ttcatcaaaa tcaaccaaga tttgtttttt tgcaaagttt aaagctccat tttgtggaac
     2101 agaacgaatt atttgaggag gaatagtatc ttttggaccg ccagttggac cttgagcttt
     2161 attagcacag gaagaaaaaa acgaaatgaa aaaataaatt ccgaaaaaat aaaggatata
     2221 tgttcttttt ctttttttat ttgtcattta attgaattta actttgcttt gtcttttcaa
     2281 aattgaagac cagaacttta aaaatattac tcagtcattt gaagcgttaa aactttatgc
     2341 ccaattctgc aaaacaaaca ttttttttct tcacaatagt tcctttttaa ttgaatcagg
     2401 gcttgtgaat caaaagctgt ttcagattca agtcccgcat ttttccaacc tttaatcaat
     2461 gcattttttt cagaaggtaa ttgttctaaa atttgtaatg atttttcctt caaatcttcg
     2521 ttgctttttt ttaatccata acaaaacaag aaaggaacaa tagtattgat taaaatgctg
     2581 ttaatagaag ctgtccctaa ttttttcgta cgttttccac ttgattctcc aaacagataa
     2641 tgtgtttgcc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntttatctgc
     2761 acaaggtatc cattgctgat tattgagtaa cgtttcataa ttggtttcaa tttttggggg
     2821 gaaagtcaaa accaattgtg gaattttaga accatcagtt cggaatactt ctccatttgc
     2881 ctcgcctacc acatgaagaa taacactatc gtaagctttg tcaaactgat gctgatgttt
     2941 taaccaatca gaagattgag aatgaatttc tacgttccct gcccacaatg tattccctat
     3001 tttgattttc gaattaaaga aatcaggacc accatctaca ttatattttc ctacatcgat
     3061 tatctcaact ttttctccat caaccgtata aagctgattt tgataaaaaa gtttatgctt
     3121 ccacacatat tgtaaaatgg cttctttcat agataaaatg aaaatctaaa agttaatcca
     3181 acttgccaat attactgaat taaattttta tatcacacca aaattataac cttttttgtt
     3241 ccagaattca attgccagag gcaatactgc cagtacatta tttcttgctt ttatggaatc
     3301 atgaaataaa ataatggaac cgtttctggt ataatgtttt acattattga atactttttg
     3361 aggtgataat ttagtgttat aatcataagt aattacatcc cataaaatga gttcatattt
     3421 ttcattaaga gctttttgct gacttctttt catttttcca taaggtggtc ggaataaatt
     3481 actgtcaatc agtcgagcag ctttttcaat attattgaga tattcttctt ttgagacaga
     3541 acaacctttc aaatgattat aagtatgatt ccccacctga tgtcctctac tcagtacttc
     3601 ttgataaagt tcaggatatt ttttcacatt ttctcctaca caaaaaaaag ttgcttttat
     3661 tccatattcg tccaataatt ttaaaataaa aggtgttact tctggaacag gaccatcatc
     3721 aaaggtaata taaatagtgt ttgaggatgg caattttctc catgtatatt tgccaataaa
     3781 aggacgtaaa agtttaggaa actgaaggtt catttacata cgaaaatgtt ttgaatactg
     3841 aacataatat ggaaaatatt tgtttgccaa atctttacgt tcagcttcat cgaaaattct
     3901 caaaacttca ttcaatatgg ccatattatt tcccatttcg ctggtaatac tgttttgctg
     3961 agtaggactt aattgtaaac tccagtttaa atattccaca cagtcattgg caatttgttc
     4021 caaaatttta tctccttttg ctggctgctg aagttgataa taatattgcc ccatcataag
     4081 agaagaataa tcatgtcgaa cgctcgtgcc gggaatcatt tcgttgcaat aatccagcac
     4141 tttctcagct ctaacggaat cattttttgc tattagggct cctgccaatt gagtaataaa
     4201 cattctatgt gttttacaca ttctgacgat gttttcatcc agatagactt ttggattatt
     4261 cacaccacca aaacggaatt tattcatcac attatcatac atttcatcta cattaacgcc
     4321 aggtccagca cctttaactc caatgggcaa aatttgataa gccaatcctg tcaattcaaa
     4381 atggtcagtc aatcccagat aataatcatc accaactgtt acagcaaaat acattggccg
     4441 ttcccatttg ttttctttca acatttcaag aaccatcaat tcactttttg taagtctatt
     4501 ttttaatgaa atatctattt gtggaataat ttcatttgct ctttcaggtt tcagtgtgcc
     4561 tgtttttaaa acttcttcag ggtcaatggg taaataaacc ttttttgtag gaataaaagg
     4621 ttctccatca atttttgtat atgagtcagt acttaaaata aaatctatca ctgttttcaa
     4681 ttgaagaggt tgattgatga catcttccac ttgaacaact tcatttttcc ccgataaata
     4741 atcctctttt ttccacgaaa tgggaagagg atcagattca tatgcctgac gtttcatctg
     4801 gtcgatatac cagtctgttt gaagataact gaggttgcaa catcttacgt ctgttctatt
     4861 accctctact tcctggttgt accataaggg aaaagtatca ttatcgccat tcgtaaaaat
     4921 aatagcattt tgtcggcatg aagccaaata attatgtcca aaatcgcgag cagtataacg
     4981 ttcactccga tcatgatcat cccagttttg agctgccatt aaagtaggaa cacctaaaga
     5041 cagcaaggtc accaaaattg agcttgatat atcagataaa tatttgttga ctgttttaaa
     5101 aagttctatt agccccaacc cgatccagat gctgaaagca taaaatgaac cggcgtaagc
     5161 atagtctcgt tcgcgaggct gataaggcgt ttgattcaaa taaactacaa tggcaatacc
     5221 tgtcatgaaa aataataaga gaactgtcca gaatcctctt ctgccttctt tacctccgta
     5281 gatttgtgaa aaaatcccca aaattcctaa caataaaggt aacatatagt aagtattatg
     5341 acctttatta gtagccagtg cagttggtaa attcttttga ttccccacca gcatgttatc
     5401 aataaaattt attcctgtaa tccaattacc tttgtccacc tctccatttc cttgtaaatc
     5461 attttgtcgc ccactgaaat tccacaggaa ataacgccaa tacatgaaat ttaactgata
     5521 attgaaaaag aaacgtagat tttcaaaaaa agtagggcac acatctgttt tttgttgtcc
     5581 acaatagtca taagtaatct gtttgccttt tactttcccc cattctttat aagcttgaat
     5641 atgatttgga gaactactgt acatacgagg gaagaacata caaaaacgtt catccatctt
     5701 ataatcaaat ttatttcctg tttttagata aatatctttt tcatctggag ttgttttagg
     5761 ttttggtgta tacatgggtt ctcctacttt tcgttcggga atgcacatat ttccttctat
     5821 acgaagttta actggtgcat tataagtggc accatagaaa agaggacgat ctccatattg
     5881 ttctcgattc aaataatatt ttaaggcaaa aacattatca ggtgaatttt catccagagg
     5941 tggattagca gaagaacgaa taactatcaa agcataagta gaataaccta tcagcattaa
     6001 cgtaatcatc aaaatagagg tatgaaggaa atgagggcta actttgcttt ttccgtgata
     6061 aaagtaaatg cctaaagctg ctattaaaat aattcccaac acaatatgtg aaccgaaaaa
     6121 tgggattcct gccaatgtaa ttgccagaag aacggaaaaa gtcattctaa agtaattttt
     6181 agctacgtac gactcatata ttgcccatgc taaagctgca atagtcaaaa taagatagac
     6241 atataaacca ctattaaatg gtaaaccgag cgtattaaca aaaaacaatt caaaccagca
     6301 agccacctgg accgatcctg gtattacacc atataatacg actgccagca aaactactga
     6361 aactaataaa gctaaaaaag ttcctttagt agaaggcgtg tattttttat aataatatac
     6421 tataacgatc gccggaattg ccagtaaatt taataggtgt acaccaatag acaaccccat
     6481 cagataagct attaataaga gccaccgatc agagcccggt tcatcggctt tttcctccca
     6541 ctttaaaatt aaccagaata ctacggctgt aaacaatgaa gaataggcat acacttcggc
     6601 ttctactgct gaaaaccaga aagtatcgga aaaagcataa gccaaagcac ctactaaccc
     6661 tgcacctatt atgataataa tattggctaa agtataattt tcttcagatt tgattaaaat
     6721 ttttttagcc agatgagtaa tcgtccaaaa taaaaaaaga atggttaaag cactacataa
     6781 tgccgacagt gtattaatca ttactgctac ttttgatgga tcggaagtga acaagctgaa
     6841 aagacgagca gtcatcataa aaaaaggagc accaggagga tgacctactt ctaatttata
     6901 agctgtagca ataaattccc cacaatccca aaaactggca gttggctcca atgttgttaa
     6961 ataagttaca aaagcaatgg caaaagc
//
LOCUS       DAOSAS010000098         6942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_7011_length_6942_cov_11.724554, whole genome shotgun sequence.
ACCESSION   DAOSAS010000098 DAOSAS010000000
VERSION     DAOSAS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7011_length_6942_cov_11.724554"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="PLR72_05500"
     CDS             complement(<1..1431)
                     /locus_tag="PLR72_05500"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOK36689.1"
                     /translation="MKKKNIIFLSLIFFTSFNVLSQNLSDVKICINPGHGGYDSDDRN
                     VVIPPFTSGNPNGFWESQSNLDKGLQLRTMLENAGATVIMTRTTNTTADDLPLSQIVA
                     IANSANVDYMLSIHSNAGNGVANYVLQLYAGKDPNDTYTYPTPTPYSEESREVSTIVA
                     QNLYSNEITNWSAPYSVRGDKTFARTAMGWSDGYGVLRGLAVPGAISEGSMHDYIPET
                     YRLMNMDYKWLEAWHFFKSFCQYFNGGEIPTGNIGGSVRDSRNVIQETYYKLKGTRDE
                     LLPLNGALITLLETNETYTTDQLNNGVYMFKNLTPGVYHVKAEANGYHSQTFETTVTA
                     NNTTYLNIDLNKVRNTPPQVISYYPNIAPPDSVECSTDIVLEFNWDMDVESTREAFSI
                     TPEVPGTITFEDSQHRLRFTPSLPLEKATLYTVKLAKTAKHPDNLSMEEDFTFQFLTK
                     NRNRLKLLIAYPYEGNHKVYTKPQFRF"
     gene            complement(1445..2365)
                     /locus_tag="PLR72_05505"
     CDS             complement(1445..2365)
                     /locus_tag="PLR72_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36690.1"
                     /translation="MKTKKIFSFLMFIFLGTYLVSAQTITITKRYKIDDNKIRGYYPI
                     LSTNGDKLLFTSDSYKGLSLYDFNTKTVIKITDEEGAGYEPIFDESESKIFYRKTSYQ
                     NNRKFEAIESFDLNTKQKKEIISPQRTIRQLQPYHKGLLVSVDRKLLKATFGKVQQEI
                     PVYVCSEDLKLVIYKNGNRQELNPIAGEDVSYIWVSLSPDKTKILFTATTKGTFVCDL
                     NGKIIASLGYLNAPVWYDNDFVVGMQDKDDGHFITESKIIMMPLKLDKKHILSLPNEI
                     AMYPSASAKAKKVAYNTLAGIIQIVELNIK"
     gene            complement(2409..4904)
                     /locus_tag="PLR72_05510"
     CDS             complement(2409..4904)
                     /locus_tag="PLR72_05510"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOK36691.1"
                     /translation="MKKIIIGFILVLAIFLQTLQAQNTITLNNRIYRIDTLVQKHKIG
                     PGAYHTYYDLPTYPLKVHIVEIDLTNPHIQIETCLANDKALTIERPSSMARRKDYPGH
                     DVVAATNGGFYFYTDPVDIGIPRSAQFINEECMVAQPYGRASFILGKNKKPFVDRFSF
                     NGLIKTGTNSHKLNMINTVNQEVYTEENANIMILYTDKFGGKTPTDNTGIEILIKPKE
                     GTTFTYRSNQDYPAVVEKILPSGGNSVIPTGKAVLFGRGNSRDFLTNINEGDEITISL
                     EFELRTTPNLLKEIKESIGGSDHKILNNGQLGEGDYGAYYGSHPRTGMGISKDSSTVY
                     LIEVDGRQPGFSMGVSLYEFAEIFKSAGAWNAVNLDGGGSSIMIVNKEIVSKFSESTE
                     RAVGNGVLIVSQAPVDNVISHIEFEPKKLEIPMYSELCPKIYGYNQYGLLINKDVKGF
                     QLSCSPELGQIINDSIFVASNIKQTGVLTATFNNLSVAKMVTIVDANINIRLDSVLID
                     HRTQYPIEVLSQNNKTIMNIPPAALSWNIRDPEICSINENGILKGLKNGSTMVIGTLG
                     DFKDSLKVIVEIPTSGRMIGDNFNVENWTLNASASLNAVLNTENLPSNWTHGAAVNYV
                     YTSTRAPFIKLLRNVPMYGLPDTLKLIINIGDINISKALLSLRANNATVSASKEFTNI
                     PKNRDTELSVAMSELFDATDIAIYPVWFDNINFYLGTQTTGQAYTLALKEIVLCYKDV
                     EISGLPSVLLSQYEVYPNPLDGTILNIRIKDSDSKLLQAQLFHLNGQSVVSQQLNPVS
                     GMATFKIGKLPAGTYLLKLYSNNETETIKVIIK"
     gene            complement(5026..>6942)
                     /locus_tag="PLR72_05515"
     CDS             complement(5026..>6942)
                     /locus_tag="PLR72_05515"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOK36692.1"
                     /translation="NELVLYNRNNGKYTRTNAYGTEVLVELIEGERWKTNSPVKAKVT
                     KIEKNKGNMPIPAGQAVLSGHGTSQTILDNLTLNDTVEITVGLKLNNSIIAPYTQMVG
                     GDNRKEMLKDGVVETSEVWGEFHPRTAIGYSKNKDTVICCVVDGRGASLGVTTKELAI
                     IMKSAGAYSAINLDGGGSSCMYVKEFGIMNTPSDGQERAVANGVFIVATSPTDNTIAE
                     IESYTKTIYLPRYGVFKPKFLGYNQYGFLINADVQGVQLSCASEVGEILEDGRFVASG
                     SNGGILTARYNNIETNIQIKLVTSAEITFRLDSVLVDHRTQYPIQVQAQVGLNIIELL
                     PAALSWTIRDSEICSIDENGYLTGLKNGTTTVVGTLGDFKDSLKVTVQIPTSGRMVGD
                     VFDAENWTLSASTALNAVLNTENLPSNWTHGAAVNYVYTSTRAPYIKLLKKVSMYGLP
                     DTLKVVANVGDINISKVLLSLKANNALTTSVSKEFTSIPQNRDTELSVAMSELFDATD
                     IAIYPVWFDNINFYLGTQTAGQAYTLALKEIVLCYRGVEISGLPSVLLSQYEVYPNPL
                     DGTILNIRIKDSDSKLLQAQLFHLNGQCVVSQQLNPVSGIAPFKIGKLPAGTYLLKLY
                     SNNEKYESVKIVIK"
BASE COUNT         2163 a         1204 c         1134 g         2441 t
ORIGIN      
        1 gaatctgaat tgaggttttg tataaacttt atgattacct tcatagggat aagcaataag
       61 taattttaaa cgatttctat ttttggttaa aaattggaaa gtaaaatctt cttccatgct
      121 taaattgtca ggatgtttag ctgttttagc taatttgacc gtatacaaag tagctttttc
      181 gaggggcaag gaaggagtaa aacgcaaacg atgttggctg tcttcaaaag taatagtgcc
      241 aggaacttct ggtgttattg aaaatgcctc tcttgtagat tccacgtcca tatcccaatt
      301 aaattctaat acaatatcag tagaacattc tacactatca ggaggagcaa tattaggata
      361 gtaagaaatt acttgtggtg gggtgtttct tactttattc aaatctatat tcaaatatgt
      421 agtattattt gctgtgactg tagtttcaaa tgtttgagaa tgataaccat tggcttcagc
      481 ttttacatga taaactcccg gagtgagatt tttaaacata taaactccat tgttaagttg
      541 atctgttgta taagtttcat ttgtttctaa aagtgttatt aaagctccat tgaggggcag
      601 taattcatct cttgttcctt ttaatttata ataagtttct tgaataacat ttcggctgtc
      661 tcgcacagaa ccacctatgt tacctgtcgg aatttcaccg ccattaaaat actgacagaa
      721 agatttaaaa aaatgccagg cttctaacca tttataatcc atattcataa gcctataggt
      781 ttcaggaata taatcatgca ttgatccttc agaaatagcc cctggaactg ctaagccacg
      841 aagaacgcca tagccgtcgc tccaacccat agctgtgcgt gcaaatgttt tatctcctct
      901 cactgaataa ggagcagacc agtttgttat ttcgttagaa tataaatttt gagccacaat
      961 ggtactaact tctcgacttt cctccgaata aggagtagga gtgggatagg tgtaggtgtc
     1021 atttggatct tttcctgcat atagttgcag cacataatta gcgactccat ttcctgcatt
     1081 actgtgaatg gataacatat aatccacatt tgccgaatta gctattgcta caatttggga
     1141 aagaggtaaa tcgtcggctg tagtatttgt cgttcttgtc attattactg tagcaccagc
     1201 attttctaac attgttctta attgcagacc tttatccaga ttactttgtg attcccaaaa
     1261 accatttgga ttaccagaag taaaaggggg tattacaaca ttcctgtcat cactatcata
     1321 acctccgtga ccaggattga tgcaaatttt aacatcagac aagttttgtg ataaaacatt
     1381 gaaggaggta aaaaatataa gggataaaaa aattatgttt ttctttttca tagcgataaa
     1441 tttattattt tatatttaat tctactattt gaataattcc agcgagagta ttataagcta
     1501 ctttttttgc tttagcagaa gcacttggat acatagcaat ttcgtttggc aaactcaaaa
     1561 tgtgtttttt gtcaagtttt aatggcatca ttattatttt tgattcagta ataaaatggc
     1621 cgtcgtcttt gtcttgcatt cctacaacaa aatcattgtc ataccaaacc ggagcattta
     1681 aatatcctaa tgaggcaata atttttccat tcaaatcaca tacaaaagtt cctttggtag
     1741 tagccgtaaa aagtatttta gttttatcag gcgacaaaga aacccatata taacttacat
     1801 cttctcctgc aattggattt aattcttgac ggttcccatt tttgtaaata accaatttca
     1861 aatcttctga acatacataa acaggtattt cttgttgaac ttttccaaag gtagctttta
     1921 ataattttct atccacagaa actaataatc ctttgtgata aggttgtaat tgtcttatag
     1981 tacgttgagg agaaattatt tccttttttt gtttcgtatt aagatcaaaa ctttctatgg
     2041 cttcaaactt acgattgttt tgataacttg ttttcctata aaaaatttta gactcggatt
     2101 catcaaatat gggctcatag ccagcacctt cttcgtcggt aattttaatt actgttttag
     2161 tgttaaaatc atataatgat aagcctttat aactgtcgga ggtaaataat aatttatctc
     2221 cattagtgct taaaatagga taatatccac gaattttatt atcatcaatt ttgtatcttt
     2281 ttgtaattgt tatcgtttgg gcagaaacta aataagtgcc taaaaagata aacattaaga
     2341 atgaaaaaat tttttttgtt ttcatagtga attattttaa tttaaattaa taataacaat
     2401 tttttatttt attttattat cacttttatt gtctcagtct cattgtttga ataaagtttc
     2461 aacaaataag ttccagcggg taatttacca attttaaaag tagccattcc acttactgga
     2521 ttcagttgtt gagaaaccac agattgacca tttaaatgaa agagttgagc ttgcagaagt
     2581 tttgagtcgc tatcttttat tcggatgttc aaaatagttc catccaatgg attcggataa
     2641 acttcgtatt gagaaagcaa tacagaaggc aatcctgaaa tttctacatc tttgtagcaa
     2701 agaacgattt ctttgagagc cagcgtataa gcttgtcctg ttgtttgtgt gcccagatag
     2761 aaattaatgt tatcgaacca gacaggataa atggcaatat ctgtggcatc gaaaagttca
     2821 ctcatggcta ctgatagttc tgtatctcta tttttaggta tattggtaaa ttcttttgaa
     2881 gcagaaacgg tagcattatt tgctctcaaa gacaaaagag cttttgaaat attaatatct
     2941 ccaatattaa taattagttt taaagtatct ggtaaaccat acatgggaac atttctgaga
     3001 agcttaataa aaggtgctct tgttgaagta tatacataat taaccgcagc tccatgtgtc
     3061 cagttgctgg gtaaattttc tgtgttaagc actgcattta aggaagcgga agcattcaat
     3121 gtccagtttt caacattgaa attatcgcca atcattcttc ctgaagttgg aatttcaaca
     3181 attactttga gagaatcttt aaaatcgccc aaagtaccga tgaccatagt actgccattt
     3241 ttcaaacctt ttaagatacc gttttcgtta atggaacaaa tttcaggatc acgaatgttc
     3301 cagctcaagg cagcaggtgg aatattcata atggttttat tattttggct taacacctca
     3361 atagggtatt gagtgcggtg atcaataagt actgaatcta atcttatatt aatgttagca
     3421 tcaacaatag tgaccatttt agcaacactt agattattaa aagtagctgt aagtacccct
     3481 gtttgtttta tgttacttgc tacaaatata gaatcgttga taatttgacc taattcaggt
     3541 gaacaactta actgaaaacc tttgacgtcc ttattaatga gtaaaccata ttgattataa
     3601 ccataaattt tgggacataa ttcactatac ataggtatct ccaatttttt tggctcaaat
     3661 tctatatgtg agattacatt gtcaactgga gcttgagata ctattaatac accgtttcct
     3721 acagcacgtt cggtactttc agaaaattta cttacaattt ctttatttac aatcataatg
     3781 gatgaacccc caccatcaag attaactgca ttccatgctc ctgcagattt aaaaatttct
     3841 gcaaattcat ataaagaaac tcccattgaa aatcctggtt gacgaccgtc aacttctatt
     3901 aaataaaccg tggaactatc ttttgaaatt cccattcccg tacgaggatg agacccataa
     3961 taggcaccat aatcaccttc tcctaattgt ccattattta aaattttatg atcacttcct
     4021 cctattgatt ctttaatttc tttcagtaga ttcggtgtgg ttcttaattc aaattccaaa
     4081 gaaatagtga tttcatctcc ttcgtttata ttggtaagaa aatcgcgtga atttccgcgt
     4141 ccaaacagaa cagctttccc ggtaggaatt actgaatttc ctcccgatgg taaaattttt
     4201 tcaacaactg ctggataatc ttggttggac cgataagtaa aagtagttcc ttcttttggc
     4261 ttgattaaaa tttcaatacc agtattatct gttggcgttt ttcctccgaa tttgtctgta
     4321 tataaaatca ttatattggc attttcttca gtataaactt cttgattaac cgtattgatc
     4381 atattaagtt tatgtgaatt ggtacctgtt ttaattaaac cattgaagga aaaacgatct
     4441 acaaatggtt ttttgttttt acctaatata aaagaagcac gtccataagg ttgagctacc
     4501 atgcattctt cattgataaa ttgggcgctt cgtggaattc ctatatcaac agggtcggta
     4561 taaaagtaaa accccccatt tgttgctgcc actacatcat gacccggata atcttttctt
     4621 cttgccattg aacttgggcg ctcgatagtc aaagctttat catttgccaa acaagtttct
     4681 atttgaatat ggggatttgt tagatctatt tcaacaatgt gaactttgag gggataagtg
     4741 ggtaaatcat aataagtatg ataagcacct ggacctattt tatgtttttg aaccaacgta
     4801 tcaatacgat aaatcctgtt gtttaaggta attgtatttt gtgcctgtaa tgtctgtaaa
     4861 aaaatggcta aaactaaaat aaaacctata ataatttttt tcataggtag tgaatatttt
     4921 taataattaa actaaagttg aataaggggt tattcaactt tagttaatga aattaatatc
     4981 aattttcttt ataaaattgt caataaattt tctattcatt tttatttatt ttatcactat
     5041 tttgacactt tcatattttt cattgtttga atagagtttc aacaaataag ttccagcagg
     5101 taatttacca attttaaaag gagctattcc acttactgga ttcagttgtt gagaaaccac
     5161 acattgacca tttaaatgaa agagttgagc ttgcagaagt tttgagtcgc tatcttttat
     5221 tcggatgttc aaaatagttc catccaatgg attcggataa acttcgtatt gagaaagtaa
     5281 tacagaaggc agtcctgaaa tttctacacc tctatagcaa agaacaattt ctttgagagc
     5341 cagtgtataa gcttgtcctg ctgtttgtgt gcccaaatag aaattaatgt tgtcgaacca
     5401 gacaggataa atggcaatat ctgttgcgtc gaaaagctcg ctcatggcta ctgatagttc
     5461 agtatctcta ttttgaggga tagaagtaaa ttcttttgat actgatgtag tgagtgcatt
     5521 atttgctttc agtgataaaa gtactttaga aatattgata tctccgacgt tggcaactac
     5581 ttttaaagta tcaggcagac catacataga aacttttttc aaaagtttaa tataaggtgc
     5641 tcttgttgaa gtatatacat aattaaccgc agctccatgt gtccagttgc tgggtaaatt
     5701 ttctgtgtta agtactgcat ttaaagcagt ggaagcactt aacgtccagt tttctgcatc
     5761 gaaaacatcg cctaccattc ttcccgaagt tggaatttgt accgttacct tgagagagtc
     5821 tttaaaatcg cctaatgtac caactactgt tgtagtgcca tttttcaatc ctgttaaata
     5881 cccgttttca tcaatagaac aaatttcaga atcacggata gtccaactta aagctgcagg
     5941 tagaagttct atgatattaa gtccaacttg tgcctggact tgaatggggt attgagttct
     6001 atggtctacc aagacagaat ctaatcggaa agtaatttcg gctgaagtaa ctaacttaat
     6061 ctgaatgttg gtttcaatat tattatagcg tgctgttaag attcctccat tacttccaga
     6121 agcaacaaaa cgtccgtctt caagaatttc ccctacttct gatgcacaac ttaattgtac
     6181 tccctgaaca tcagcattaa tcaaaaatcc atactgatta tatcctaaaa attttggttt
     6241 aaaaactccg tatctaggta aataaatagt tttggtatac gattcaattt cagcgatagt
     6301 attgtctgta ggagaagtag ccactatgaa aacgccattt gcaacagctc gttcctgtcc
     6361 atcactgggg gtattcataa tgccaaattc ttttacatac atacaactgg atccaccacc
     6421 atccagatta atagccgaat aagctcctgc acttttcata attatggcta attctttggt
     6481 agtaacgcct aaagaagcac ctcgcccatc aactacgcaa caaataaccg tatctttatt
     6541 ttttgaatat ccaatggctg tacgaggatg aaattcaccc caaacttcgg atgtctctac
     6601 aactccatct tttaacattt cttttcggtt atctccacct accatttggg tgtaaggagc
     6661 aataatacta ttatttaatt ttaagccgac tgtgatttcc accgtgtcat ttaaagtaag
     6721 attgtccaaa attgtttgcg aagttccgtg tccggaaagt acagcttgcc ctgcaggaat
     6781 gggcatattt cctttgtttt tttcaatttt ggtaactttt gcttttactg ggctatttgt
     6841 tttccaacgt tctccttcaa ttaattcaac caaaacttct gtcccataag cattggtacg
     6901 agtatatttt ccattgtttc tattgtaaag aaccaattca tt
//
LOCUS       DAOSAS010000099         6926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_7033_length_6926_cov_14.106389, whole genome shotgun sequence.
ACCESSION   DAOSAS010000099 DAOSAS010000000
VERSION     DAOSAS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7033_length_6926_cov_14.106389"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..842)
                     /locus_tag="PLR72_05520"
     CDS             complement(195..842)
                     /locus_tag="PLR72_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOK36693.1"
                     /translation="MCKFTTFLLIFIILSLYLSAQNPDIWILEQVNDEHPKMLRKYSL
                     MVTNTATTITLSTPFVIGMIGIIDKNSQLEKDALYVGATLGVEGILTLGLKQAVHRER
                     PYVTYPNVVFPYQILPSYSFPSGHTSMAFANATALSLKYPKWYVIIPSYFWACSVGYS
                     RLNLGEHYPSDVLAGAIVGAGSAFLSLKLTNWLFNSCENKKQCKTLNIFNTYLSF"
     gene            1101..1625
                     /locus_tag="PLR72_05525"
     CDS             1101..1625
                     /locus_tag="PLR72_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK36694.1"
                     /translation="MKKIAIIGPESTGKTELAKKLAEHFKSPWEPEYAREYIEKLTAP
                     YTYEDICNIARYQINQEKFYEEHPEINADYVFFDTDLIITKVWFQYKFGKVPHFLIER
                     LNQRFFDFYFLCYPDLAWQPDPVREHGNDREFFFDWYRKEIEELGKPYFVVKGFGDQR
                     IQCAINALLSFRNK"
     gene            1638..2528
                     /locus_tag="PLR72_05530"
     CDS             1638..2528
                     /locus_tag="PLR72_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOK36695.1"
                     /translation="MKNEILETIQKLSQIEKIKDVCHKQGLNFPMPSINTLQEIVQLC
                     RSILFPGYFDEAPLNSETLKFHIGVNVDHLLMLLSKEIKAGICFNGNTKITTEKCEEL
                     DKISLQKAVEFILKLPEIRQKLYTDVIAIYNGDPAAKSFGEVIFCYPGLKAITNYRLA
                     HALLKLDVPLIPRIITEMAHSETGIDIHPGAEIGEYFMIDHGTGVVIGETSVIGNNVK
                     MYQGVTLGAKSFPLDENGNPIKGIVRHPKIGNNVIIYSNSTILGNITIGDGAVIGGNL
                     WVDKDVPAGARLSQDKNLNF"
     gene            2541..3749
                     /locus_tag="PLR72_05535"
     CDS             2541..3749
                     /locus_tag="PLR72_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOK36696.1"
                     /translation="MKKTLQTPDKEITILSTEEFPMIAKTFQGLEEVLAEELTSIGAS
                     NVQIQRRAVSFTGDKALMYKANLHCRTALRILKPIFTFNANDADEVYEQIKKFDWGQL
                     MTVESTFAIDATVFSEEFTHSKYVVYRVKDAIADWFMERYHRRPSVSVTSPQLMFNIH
                     IAGRHCTLSLDSSGESLHKRGYRVAQTEAPINEVLAAGMLLLAGWHGQSDFLDPMCGS
                     GTLLIEAALIALNMPPGIFRPSFAFEKWSDFDKELFDDLYNDDSQERAFEHKIYGSDI
                     SGRAIKIAEQNVKSSGLSKYIQLKVAPLEKLEPPFSHCFIVTNPPYGERLPSKDIFDL
                     YASLGSILKHKFSGNTAWIISSNEEALKKIGLKPSKRFQLLNGPLECWFQQYELFAGK
                     RNEYLQKKNA"
     gene            3876..3959
                     /locus_tag="PLR72_05540"
     tRNA            3876..3959
                     /locus_tag="PLR72_05540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3910..3912,aa:Leu,seq:caa)
     gene            complement(4142..4750)
                     /locus_tag="PLR72_05545"
     CDS             complement(4142..4750)
                     /locus_tag="PLR72_05545"
                     /inference="COORDINATES: protein motif:HMM:NF040252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36697.1"
                     /translation="MKKLYFILFLIMNMLLTNYANGENPFFQNYKTPFGVPPFDKIKT
                     EHYLPAFQAGIKQHQNEIDKIATNKLAPTFDNTVAALDYSGELLKKVSYVFFNLYSTD
                     SNEQMEAIAEQITPQLTEHNDNLYLNEALFKRIKTLYEQKDKLGLSFEQNRLLEKYYK
                     NFVRNGAALNAEQKAQLREINKELSLAELQFGQNVDRFLIFS"
     gene            complement(4820..5746)
                     /locus_tag="PLR72_05550"
     CDS             complement(4820..5746)
                     /locus_tag="PLR72_05550"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOK36698.1"
                     /translation="MQIINSPSPASFPSCVATVGFFDGVHVGHRFLIEKLKNIAYEEQ
                     LPSVVITFDTHPRKVLHSDFQPKLLTTFEEKMEQLSTTGVDICAVLNFTREMSELSAY
                     EFLKDILRDLFHVQTLLVGYDHRFGHNRAEGFEEYQKYGEELGIKVMRAERYSTPELV
                     HVSSSIIRKALERGDIDLANRLLTYPYSFSGKVKNGFKVGRTLGFPTANLEPLDCDKL
                     LPAVGVYAVRVKYDEMFYKGMMDIGFRPTFGDSHQLFIEVHIIDFDKNIYQEILKIEF
                     IQRLRDEIKFNNVEELIEQMEKDKAKVLALNF"
     gene            complement(5753..6418)
                     /locus_tag="PLR72_05555"
     CDS             complement(5753..6418)
                     /locus_tag="PLR72_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOK36699.1"
                     /translation="MESFKNIKAIIFDLGGVVLDLDRDRCIRNFHSLGVNGIEKFLND
                     YLPAGFLLQLEKGKISPEEFRNEIRKFTPQKLTDEQIDKAFCSFLVDIPSERLQLLLD
                     LQKRFKLYLLSNTNAIHMDYCKKNFFQWKGHQLEDFFNKCFFSYELGLTKPDPEIFKV
                     VLQEAKLQPHECLFLDDGIINIEEASKLGIQTHLVKEGEDLNFLLQPSTFIFEPLTVE
                     TKH"
     gene            6513..6587
                     /locus_tag="PLR72_05560"
     tRNA            6513..6587
                     /locus_tag="PLR72_05560"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6548..6550,aa:Ile,seq:cat)
     gene            complement(6655..6831)
                     /locus_tag="PLR72_05565"
     CDS             complement(6655..6831)
                     /locus_tag="PLR72_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOK36700.1"
                     /translation="MDAKALVLEAMKKAGEPVNASKIVELTGLDKKAVDKAMNELKAE
                     GAIESPKRCYWQPK"
BASE COUNT         2298 a         1174 c         1150 g         2304 t
ORIGIN      
        1 aggcaaacct cgaggaattc ttttgattaa agaggagcaa aatttgctaa aacaattaaa
       61 atgaaaccct tttgaggcaa aaggaaataa gttcacagca atgttttacc ttgaaaaaat
      121 tccttctctt ttttctgaat aatgtcatgc atcatattat ttcaaagctt tatattctcg
      181 aatacaactg agtatcaaaa agaaagataa gtattaaaaa tattaagtgt tttacactgt
      241 tttttgtttt cgcatgaatt aaataaccaa ttggtcaatt ttaaggataa gaaagcactt
      301 ccagctccta caatagcgcc agctaataca tcagaaggat aatgttcgcc cagatttagt
      361 cgggaatagc cgacagagca agcccaaaaa taacttggaa ttatcacata ccatttagga
      421 tattttaaac tcaaagcagt agcattggca aaagccatag aagtatgccc cgatggaaaa
      481 gaataagaag gcaagatttg ataaggaaaa actacgttgg ggtaagttac ataaggacgt
      541 tccctgtgaa cagcttgttt taaccccaaa gttaatattc cttcaacacc taatgtagct
      601 ccaacatata atgcatcttt ttccaattgt gagtttttat caattattcc tatcattcct
      661 atcacaaaag gagtagaaag tgtaatagtt gtagcggtat tcgttaccat gagagaatat
      721 tttctcaaca ttttgggatg ttcatcgttt acttgttcta aaatccagat atcaggattt
      781 tgagcagaaa ggtataaaga taaaataatg aaaatcaata gaaatgttgt aaatttgcac
      841 atatttttga tgaatttaag tagatgatag aattgatgtt tttagaaatc agaattttaa
      901 ctaagatatt tcacttttta ctcctgttta aattttttgc tatggaattt ttcatctttt
      961 aataaaacgt taatttaaag taaaaaacta cctttaaata ttcattctaa caatttaaaa
     1021 tttactctag tgtaacacaa tttttagttg tatggttttt aaaagggaaa aaaattacag
     1081 atagacaaat tgattacttt atgaaaaaaa ttgccattat tggtcctgaa tcaacaggaa
     1141 aaacagaatt agcaaaaaag ctggcagaac attttaaatc cccgtgggaa cctgaatacg
     1201 cgcgtgaata tattgaaaaa ttgactgctc cttatactta tgaagatatt tgcaacattg
     1261 caagatatca aattaaccaa gaaaaattct atgaagagca tccagaaata aatgctgatt
     1321 atgttttttt tgatacagat ttaattatta caaaagtatg gtttcaatac aagttcggga
     1381 aagttcctca ttttttaatt gagagattga atcagcgttt ttttgatttt tactttcttt
     1441 gttatcccga tttggcatgg caacctgatc cagttagaga acatggaaac gatcgtgaat
     1501 ttttctttga ttggtatcga aaggaaattg aagaattagg aaaaccttat tttgtggtca
     1561 aaggttttgg tgatcaacgg attcaatgtg ctataaatgc cttattatca tttcgtaata
     1621 aataaaaata taaaaatatg aaaaatgaaa ttttagaaac tattcaaaag ttatcccaga
     1681 ttgaaaaaat caaggatgtc tgccacaaac aaggattaaa ttttcctatg ccttccatca
     1741 atacattgca agaaattgtt cagttgtgtc gttctattct ctttccagga tattttgatg
     1801 aggctccttt aaattcagaa acattaaaat tccatattgg agtgaatgtt gatcatttgt
     1861 tgatgctgct ttcaaaagaa atcaaagcag gaatatgttt taacggaaat accaaaataa
     1921 ctaccgaaaa atgtgaggag ttggataaaa tttctctgca aaaggctgtt gaatttatat
     1981 tgaaactgcc tgaaataagg caaaaacttt ataccgatgt gattgctatt tataatggcg
     2041 atcctgctgc aaaaagtttt ggcgaagtta ttttttgtta tcctggacta aaagcaatta
     2101 ccaattatcg actggctcac gctttactga aacttgatgt tcccttaatt ccacgaatca
     2161 tcacagaaat ggctcattca gaaacaggta ttgatattca tccgggagca gaaattggag
     2221 aatattttat gattgatcat ggaacgggtg ttgtcattgg tgaaacttct gtcattggta
     2281 ataatgtaaa aatgtatcaa ggagttacgc ttggagccaa gagctttcca ttggacgaaa
     2341 acggaaatcc tatcaaaggc attgttcgac atccaaaaat tggaaataat gtgattattt
     2401 attccaattc tactattctg ggaaatatta ctattggaga tggagcagtg attggcggaa
     2461 atctctgggt tgataaggat gtcccggctg gtgcgagatt atcacaggat aaaaacttga
     2521 atttttgatt tttattttta atgaaaaaaa ctttacaaac tccagataaa gaaattacta
     2581 tactttctac agaagaattt ccaatgattg ccaaaacttt tcaaggattg gaagaagttt
     2641 tggcagaaga gcttacttca attggtgcca gtaatgttca aatacaacgc cgagcagtca
     2701 gttttacagg agataaagct ttgatgtaca aagcaaattt acattgtcga acagccttac
     2761 gtattttgaa gccaattttt acctttaatg ctaatgatgc tgacgaagta tatgaacaga
     2821 taaaaaaatt tgactggggt caattaatga cagttgaaag cacatttgct attgatgcaa
     2881 cagtattttc agaagaattc acacattcaa aatatgtagt ttacagagta aaagatgcta
     2941 ttgccgattg gtttatggaa cgttatcatc gtcgtccgag tgtttcagtt acctcgccac
     3001 aattaatgtt taatattcat attgctggcc gtcattgcac cctttcattg gacagttctg
     3061 gagaatcgct tcacaaacgt ggttatcgtg ttgcacaaac tgaagctcct attaacgaag
     3121 tgctggcagc aggaatgttg ttattggctg gctggcatgg gcaatccgat tttctcgatc
     3181 ccatgtgcgg atctggtaca ttgcttatag aagctgcctt gatagcatta aatatgccac
     3241 caggcatttt cagaccttct tttgcttttg aaaaatggtc agactttgat aaggaacttt
     3301 ttgatgattt gtacaatgat gattcgcaag aaagagcttt tgaacataaa atttatggat
     3361 cggacatttc tggaagagct ataaaaattg cagaacaaaa tgtaaaaagc agcggacttt
     3421 caaaatatat tcagttaaaa gttgctcctc ttgaaaaact tgaaccgcca ttttcacact
     3481 gctttattgt gaccaatcct ccttatggtg aacgacttcc gtcaaaggat atttttgatt
     3541 tatatgcttc tttaggttcc attttgaagc acaagtttag tggaaatact gcctggataa
     3601 ttagttccaa tgaagaagct ttgaaaaaaa ttggtttaaa accatcgaaa cgatttcaac
     3661 tgctcaatgg acctttggaa tgctggtttc aacaatatga acttttcgca ggaaagcgta
     3721 acgaatattt gcaaaaaaag aatgcataaa ttctatttat ttttatagct gattgattat
     3781 caataaaatt attctccttt cgagaaagaa aagaattttc cgcagttttt gtttttgaga
     3841 ataaaaaaaa tgatttactt ttgtagttgt ttaatgccca gatggcggaa ttggtagacg
     3901 cgctggtctc aaacaccagt ggattcacct ccatgccggt tcgatcccgg ctctgggtac
     3961 tcctgaaaag ctcaattagt tggtaatcaa ttgttgagct tttttatttt ctctatttgc
     4021 accacatagt aatttgttcc ttttccttat tttgccatat tattccctta atttcctgtt
     4081 gtttaagcta atgcctaacc gctatcatat ataatatttt ttgtatcttt acaagaaaaa
     4141 gtcatgaaaa tattaaaaac ctatccacat tttgaccgaa ttgcaattca gcaagcgata
     4201 attctttgtt aatctctcga agttgagctt tttgttccgc atttaaagca gctccattcc
     4261 gaacaaaatt cttgtaatat ttttccagca aacgattttg ttcaaaactc aaacccaact
     4321 tgtccttttg ttcataaagc gttttaattc ttttaaaaag tgcttcattg agatataaat
     4381 tatcgttatg ttccgtgagc tgtggtgtga tttgttcagc aatggcctcc atttgctcat
     4441 ttgaatctgt ggaataaaga ttgaagaaga catacgaaac ttttttaagc aattcgccac
     4501 tataatccag tgcagctact gtattatcaa aagtaggagc taatttgttg gtagcaattt
     4561 tatcaatttc attttgatgt tgtttaattc ctgcctgaaa agcaggaaga taatgttcag
     4621 ttttgatctt gtcgaaagga ggaaccccaa aaggagtttt gtaattctga aagaaaggat
     4681 tttcaccgtt cgcataattt gttaacaaca tattcattat caaaaataaa ataaaatata
     4741 actttttcat attcaacatt tttcagaagg aaatacatca cgaagataaa tattagcata
     4801 agagaataaa gaaagtcttt taaaaattca atgctagcac tttggcttta tctttctcca
     4861 tttgttcaat taattcctcc acattattga attttatttc gtctcttaat cgctggataa
     4921 attcaatttt caatatttct tgataaatat ttttgtcaaa atcaataata tgaacttcaa
     4981 tgaaaagttg atgactatcg ccaaaagtgg ggcgaaaacc aatatccatc attcctttat
     5041 aaaacatttc atcgtatttc acccgaacgg catatacgcc aacggctggc aataatttat
     5101 cacaatcaag tggctcaaga tttgccgttg gaaaacccag tgtgcgaccg actttgaaac
     5161 catttttcac tttccccgaa aacgagtacg gataagttag caatcgattg gcaagatcaa
     5221 tgtcacctct ttccaatgct ttccgaataa tggaagaact cacatgcacc aactctggag
     5281 ttgaataacg ttctgctcgc ataactttga tccccaattc ttcgccatat ttttgatatt
     5341 cttcaaatcc ctctgctcga ttatgcccaa aacgatgatc atatcccacc aataaagtct
     5401 gaacatgaaa caaatcacga agaatgtctt ttaaaaactc ataagctgaa agttcagaca
     5461 tttctctggt aaaatttagc accgcacaaa tatccacacc tgtggtggaa agttgttcca
     5521 ttttttcttc aaaagtggtt aacaattttg gttgaaaatc agaatgtaaa acttttcgtg
     5581 gatgagtgtc aaaagtaata accacactcg gcaattgttc ttcgtaagcg atgtttttca
     5641 atttttcaat taaaaaccga tgacccacgt gtacaccatc aaaaaagcct acagtagcta
     5701 cacatgatgg aaaagaagct ggggaaggag aatttatgat ttgcataata attcaatgtt
     5761 ttgtttcaac cgttaacggc tcaaatataa aagtggaagg ctgaagtaaa aagttcaaat
     5821 cttctccctc ttttactaga tgagtctgaa tacccaattt acttgcttcc tcaatgttta
     5881 taataccatc atccaggaac aaacattcat gaggctgtaa ctttgcctct tgcagaacaa
     5941 ccttaaaaat ttctggatct ggtttggtta gacctaattc atacgagaaa aaacatttgt
     6001 taaaaaaatc ttccagctga tgacccttcc actgaaaaaa attcttttta caataatcca
     6061 tgtgaatggc attggtattg ctcaaaagat aaagtttgaa tcttttttgt aaatccaaca
     6121 ataattgaag ccgttcagat ggaatatcaa ccaaaaaaga gcaaaaagct ttatcaattt
     6181 gttcgtctgt taacttttga ggtgtaaatt ttcgtatttc attcctgaat tcttctggcg
     6241 aaatttttcc tttttccagt tgaagtaaaa agcctgctgg aagataatca ttcaaaaatt
     6301 tttcaattcc attcacaccc aatgaatgaa aatttctaat acatctgtct ctatctaaat
     6361 ccagcacgac gccacccaga tcaaaaatga tggcttttat atttttaaat gattccattg
     6421 ttcagtaaaa tgaagataat tttttttgaa tagcaaaatt tattattttt gtcatcgctt
     6481 tgcaaagata cgtttttttc agggaaatgt gaggacccat agctcagtcg gttagtagca
     6541 cctgactcat aatcaggggg tcgctggttc aagtccagct gggtccacaa aaaataaaag
     6601 agactgatca tcaccaacat atcagtctct ttttctttaa aaaacctttc attttcattt
     6661 cggttgccaa taacatcttt taggtgattc tatggcacct tcagctttca gctcgttcat
     6721 ggctttgtcc accgcttttt tatccagtcc ggtcaattct acaattttgc ttgcattcac
     6781 cggttctcca gccttcttca tggcttccaa tactaacgct tttgcatcca taactttatc
     6841 ttttaatttc ttaataaaca caagattatg gcaaattgtt ttttagaata aaaaataatt
     6901 tttatccgat tgtcattaaa attaaa
//
LOCUS       DAOSAS010000100         6846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_7124_length_6846_cov_13.599028, whole genome shotgun sequence.
ACCESSION   DAOSAS010000100 DAOSAS010000000
VERSION     DAOSAS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7124_length_6846_cov_13.599028"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..401
                     /locus_tag="PLR72_05570"
     CDS             198..401
                     /locus_tag="PLR72_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36701.1"
                     /translation="MRQLILIFILIVSTTFTVCGQSDSNKAKLNAQTDFEEGNYFFHS
                     LEFSSLPHHCIIWGSKKEINFLK"
     gene            complement(635..874)
                     /locus_tag="PLR72_05575"
     CDS             complement(635..874)
                     /locus_tag="PLR72_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36702.1"
                     /translation="MAGIGISIYELLSKSEKKGNSKKKQVIYNGVLLIILGIYLLITK
                     MFLEKTLLTISWLIGVFVIVGIIRKSKKNQSNLKT"
     gene            complement(1631..2401)
                     /gene="kdsB"
                     /locus_tag="PLR72_05580"
     CDS             complement(1631..2401)
                     /gene="kdsB"
                     /locus_tag="PLR72_05580"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOK36703.1"
                     /translation="MAITLDSVVFVGIIPARYASSRFPGKPLADIGGKSMIQRVYEQA
                     SKVLSLVYVATDDQRIFDVVKAFGGKVIMTSTEHKTGTDRCYEASTKIKENFDVIINI
                     QGDEPFIQPEQIVTLQNCFHDNTTEIATLAKLVTIQDGFEYLANPNRPKIVLNKNNEA
                     LYFSRSVIPYIRGKATSEWIENHKFYTHIGMYAYRKDVLEKIHSLPPSSLETAESLEQ
                     LRWLENGFRIKVGFTDKVSFSIDTPEDLEEARKLFKSL"
     gene            complement(2419..3609)
                     /locus_tag="PLR72_05585"
     CDS             complement(2419..3609)
                     /locus_tag="PLR72_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOK36704.1"
                     /translation="MKRIFLVVIINLNSFYLFSQLPLFSDSTVISLLTCSPGKEVYAK
                     FGHTAIRLNDPLTGNDLIFNYGVFNFDTKNFYLKFIKGETDYQLAVYDTQSFLQEYIA
                     RNSMVWEQILNLTPQEKKKLIHSLLVNYEPQNRIYRYNFVYDNCSTRPRDKIVEALDG
                     KIDYTGFFEEKTFRDLIGIYTGENSWLKFGIDLILGADADKVATRNESMFLPEMLMAQ
                     FQSAKIISNFNSTRPLIAENKIIVQKNPEKLSQTFPLFLPISVCLLLFLVAITCTVLN
                     NKRSFRVFDSIAYIVSGLMGLIIFYLMFFSAHPLVKSNFNILWLNPLNLFAGVFLWIR
                     KTKPILFYYQILNSLFLFIALIVFILGIQEFNIASIFLILTLLLRTVRWVVTTRKDFI
                     FKNK"
     gene            3863..4564
                     /locus_tag="PLR72_05590"
     CDS             3863..4564
                     /locus_tag="PLR72_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOK36705.1"
                     /translation="MTRKQGFELRPIEDVFSVWDVLTPDERQFVKSNYITQCFKKNEL
                     IQCEGEIPTHMMILASGKAKVFKDGIGSRTQIIRMLKPGENFGYRAIIANEPNSTTVA
                     AFENSAVYMIKADIFLSILRHNNAVCYRFLEEMASDLADSNTRTVNLTQKHIRGRLAE
                     ALLFLKKNYGVEGDGATINIYLSREDLANLSNMTTSNAIRTLSSFANEHIIAVDGRKL
                     KIIDEDALKRISKMG"
     gene            4648..>6846
                     /locus_tag="PLR72_05595"
     CDS             4648..>6846
                     /locus_tag="PLR72_05595"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK36706.1"
                     /translation="MNATQIETTYNTALRFLSNFNLKNAFDKCKILINETQFGEFKDR
                     CDNLEQNYKLLLSFFVSGAKDPERETIYRKLIARLFVLLSDVKEELLVQNSTNFEYSQ
                     KRYFPYMRNHKTATAIYNALSYYHKQTSLLLETAQLHEVEWKRLRSNYEATLGELFNL
                     FWLTTNFDEDEKNLFQNILEANYEGSVEKSLIVSALTLNLWRKFDENKLMMLFDSSQC
                     ENPHARQRALVGLCFILAKYNTFLPYFPSVKNRLVLMTDDHHLVENFQNIIIQIIGTT
                     ETEKISKKLHEEILPEVMKLNPLLKDKMDAENFLNSDEWMEENPEWQEILQESGLSDK
                     LQELSDLQLEGADVYMSTFSMLKSFPFFSEFYHWFLPFDPENSAINELFKEEEKTLLS
                     AFVNSNIMCNSDKYSFCLSILQMPESQRNMLKQSFKLESNQLEEMEKETALLSPETVE
                     KNISKQYIQDLFRFFKLHPQHNDFEDMFATSLSLHRTYLFEILATDSNFKLNIAEYYF
                     SKGYFRQALDLFEQLQSEIQPSASLYQKMGYSYQQTSQLQKALEAYLKADIIQPDDEW
                     TIRKIALCYRLLGNQEKALQFYQYADFLNPNQYTIQRHIAQCYVELGKYQEALNIYYK
                     LDAENGDDIKIWRAISWCSFVMGDLKKAEYYSQKILASTPVAYDFINGGHIAWSQGKI
                     SEALELYCKGLNLLQNNWEVFQDTFFEDKSHLLARGIKENEIPLMLDEILY"
BASE COUNT         2359 a         1073 c         1068 g         2346 t
ORIGIN      
        1 actcaatatt gacaatggtt tgtaagaacg agtggacaga acaccagctg ccaacagcgt
       61 gtatgtggca atagcgggtg tagtggcaag aaggtctgtg cttctaagaa actttgcgct
      121 aaacggacag gaaagtacct ctaatccgct actgcacata cacgccaacc gttgtggtgc
      181 attttaaaaa gacgacaatg agacaactaa tactcatttt tatccttatc gtttcgacaa
      241 cttttacagt ttgtggacag tcggacagca ataaagccaa attgaatgct caaacagatt
      301 tcgaagaagg aaattatttt tttcattcat tagaattttc ctcgctcccg caccattgca
      361 tcatttgggg aagtaaaaaa gaaataaatt ttttaaaata aacagtttta aaaacttact
      421 acaagaagaa tttgtgcggg gcagcaaagg ataatttttt aattttttca aaattaatca
      481 agttttcatt cggatgctct tttttctcat tgttccgttt cgctacgctt cacttcacaa
      541 tgagaaaaaa gtatcaacaa cgtgatgaaa ctttacactt aataaaataa ggattaaaat
      601 aagttttgtt agatgttgtc ttagtaaaga atatttatgt ttttaaatta gattgatttt
      661 tttttgattt acgaataata cccactatta caaaaactcc tattaaccag cttatagtta
      721 ataaagtttt ttctaaaaac atttttgtga ttagtaagta aattcctaaa ataatgagca
      781 aaacgccatt atatataact tgtttttttt tgctattacc ttttttttct gatttagata
      841 acagctcata aatcgaaata cctatacctg ccacaattaa aagcaatgga atccaataca
      901 atttaattta ataaaatcca taatttatca atttaaaagt tgaataaaat aaatattgag
      961 ttaattgtct tatctgacat tgaatatttt acattttaca tctattcctt ttatgatctc
     1021 tatgtgtcaa gtttcacaag gttgtttatg aaaacttaga ttttgaattg ggtttaaaag
     1081 tataatttta cttttaaatt cagaaggatt ttgtttaaaa atttcaggtt tcatttcaaa
     1141 ccatttttca actacttgaa aaggagtttt cacgtttagt tcttttctct tagtccacta
     1201 tgtcttctat aaagcatata gtagacaaga aaagccatca gcgcattatt catttttttc
     1261 tttatttacg taattgtctt taagaatgtt ttaataattc cattggctct ttccaccatg
     1321 ccattcgttt ttggggtatt gggctgagta agccccaccg ttctgtcgga tttgcaatct
     1381 gacagaatta tttgcggatt ttaaatctac tattaaacca gcaaaggatt acaaattctt
     1441 tgtgacagag aaaagatttt ttgacaccaa cttctcttat ttttcggctc accggacaag
     1501 gtcatctctt tattcttatt attctgtatt tcaagcatta atatttaaaa actcgcaaac
     1561 tccaaactcg tatcccgcac ctcgtcaact ctttttatac ttaaaaacaa ttcgatacaa
     1621 aacaagaatt tcataaactt ttaaataatt ttcttgcttc ttctaaatct tcaggcgtgt
     1681 caatgctaaa agatacttta tctgtaaaac ctactttaat acgaaaacca ttctccagcc
     1741 agcggagttg ttcaagcgat tcggctgttt caagcgatga tggtggcaag gaatggattt
     1801 tttccaacac atcttttcga taagcataca tgccaatatg tgtgtaaaac ttgtgatttt
     1861 caatccattc cgaagtggct tttccacgaa tgtatggaat cacggaacga ctaaaataca
     1921 aagcttcgtt atttttgttg aggacaattt taggacgatt gggattggca agatactcaa
     1981 aaccatcttg aatggtaact aattttgcaa gtgttgcaat ctctgttgtg ttgtcgtgaa
     2041 aacaattttg caatgttaca atttgttcag gttgaataaa aggttcatct ccttgaatat
     2101 taataattac atcaaaattt tcctttattt ttgtagaagc ctcatagcaa cgatctgttc
     2161 cagttttatg ttctgttgaa gtcataataa ctttcccgcc aaaagctttt actacatcaa
     2221 aaattcgttg atcatcagtt gctacgtaaa ctaatgacaa aactttgctg gcttgttcat
     2281 atactctttg aatcatactt ttaccaccaa tatctgccaa tggtttaccc ggaaaacgac
     2341 ttgaagcata acgtgcggga atgataccta caaaaacaac agaatccaaa gttattgcca
     2401 ttttgttata gaaaaaattt atttattctt aaaaatgaaa tcttttcttg tcgttacaac
     2461 ccatctgact gttcgtaaaa gaagcgtcaa aatcaaaaag atagaagcaa tgttaaattc
     2521 ttgaatacca agaataaaaa ctattaatgc aataaatagg aacaaagagt tcagtatttg
     2581 ataataaaac agaataggtt ttgtttttct gatccataaa aatactcctg caaataaatt
     2641 aagaggattt agccataaaa tattaaaatt ggattttacc agcggatgag ctgaaaagaa
     2701 cataagataa aaaataatca accccattaa tcctgaaaca atatacgcaa tgctgtcgaa
     2761 aactctgaaa ctgcgtttgt tattcaaaac agtacaagtt atagctacca aaaacaacaa
     2821 caagcataca gaaattggta aaaacaaagg gaaagtttga gataattttt caggattttt
     2881 ttgtacaata attttatttt cagcaattaa tggccttgtt gagttaaaat tagaaataat
     2941 ttttgcggat tgaaattgag ccattaacat ttcaggtaaa aacatacttt catttctcgt
     3001 tgcaacttta tcagcatcag ccccgagaat aagatcaatt ccaaatttca gccagctatt
     3061 ttcacctgta tatattccta tcaggtcacg aaaagttttt tcttcaaaaa agcccgtata
     3121 gtcaattttt ccatccagag cttctacaat cttatcgcgt ggacgagtgg aacaattatc
     3181 ataaacaaaa ttgtatcgat agattctatt ttgtggttcg taattgacta ataatgaatg
     3241 aataagtttt ttcttttctt gtggagttaa attaagaatt tgttcccaca ccatagaatt
     3301 gcgagctata tattcttgta aaaaactttg tgtatcgtaa acagctaatt gataatctgt
     3361 ttctcctttg ataaatttca gataaaagtt tttggtgtca aagttaaaaa ctccatagtt
     3421 gaaaattaag tcattacctg ttaaaggatc gtttaatcgg atggcagtat gcccgaattt
     3481 agcataaact tcttttcccg gcgaacaagt aagaaggctt atgacagtag aatcgctaaa
     3541 caacggcaat tgagaaaaca gataaaaact atttaaatta ataataacaa ctaagaatat
     3601 ccttttcatc tcttttcaat tttttttaac ttttgcaaac aaaggtaatt aattttttta
     3661 ttgtttataa aactagaaaa atattgtttt atactaaaag tgaactgaaa attttctaat
     3721 gttttatttt tttatgatat tttatccatt tgttttttaa agggacatca gtgttttttt
     3781 cattttagta caaaagtgat aaaaaaatca ttaaatttgt gctttataac attattgtca
     3841 aacatattcg ttttaaaatg tgatgacaag gaaacaaggt tttgaacttc ggccaatcga
     3901 agatgtgttt tctgtctggg atgtgctcac acccgatgaa agacaatttg tgaaaagtaa
     3961 ttatattact caatgtttca aaaaaaatga gttaattcaa tgcgaaggag aaattcctac
     4021 gcacatgatg atcctggctt caggcaaggc aaaagttttt aaagatggca tcggaagtcg
     4081 cacccaaatt attcgtatgc ttaaaccggg agaaaatttt ggctaccgtg ctattatagc
     4141 aaatgaacca aatagtacta ctgttgcagc atttgaaaat tctgctgtat acatgataaa
     4201 agccgacatt tttttatcta ttttacgaca caataatgcc gtttgttatc gtttcctcga
     4261 agaaatggct tctgatctgg ctgattctaa tacccgtact gtcaatctga cacaaaaaca
     4321 cattcgtgga agattggcag aagctttgtt attcctaaaa aagaattatg gagtagaagg
     4381 agatggagcc accattaaca tttatctttc aagagaagat ttggccaact tatctaatat
     4441 gacaacatca aatgctatcc gaaccttatc aagctttgca aatgaacata tcattgcagt
     4501 ggacggtcga aagctaaaaa ttatcgatga agatgccttg aaaagaatta gtaaaatggg
     4561 ctaaatattt gtagaaaatt ttacaaggtt aagacatctt ttcattcctg ttcttaaatt
     4621 atcaagatca ctcaaagtgt actttttatg aatgcgactc aaattgaaac aacttataat
     4681 acagcgttga gatttctttc taactttaat ctgaaaaatg cttttgataa gtgtaaaatt
     4741 cttattaatg aaacccaatt cggagaattt aaagatagat gcgataattt ggaacaaaat
     4801 tataaattgc tgttaagttt ttttgtgagt ggagcaaaag atccagagcg ggaaacaatc
     4861 tatcgaaaat taattgcacg tctgtttgtt ttactgtcag atgtgaaaga agaattatta
     4921 gttcaaaatt ctactaattt tgaatattct caaaaacgtt atttccctta catgcgtaat
     4981 cataaaacag caactgcaat ttataatgct ctttcttatt accataaaca aacttcattg
     5041 ttgttagaaa ctgcccaatt gcatgaagtg gaatggaaaa gattacgttc caattatgaa
     5101 gctactttag gtgaactatt caacttattt tggttgacaa caaattttga tgaggatgaa
     5161 aaaaatcttt ttcaaaacat actggaagcc aattacgaag gaagtgttga aaaatcttta
     5221 atagtttctg ctctcacttt gaatttatgg agaaaatttg atgaaaataa actcatgatg
     5281 ctatttgata gttctcaatg tgagaatcca catgccagac agcgtgcctt agttggatta
     5341 tgctttatat tggcaaaata taatactttt ttgccttatt ttccttctgt aaaaaatcgt
     5401 ttagttttaa tgactgatga tcatcatctt gttgaaaatt ttcaaaatat aatcatccaa
     5461 attattggta ctactgaaac tgaaaaaatt tccaaaaaac ttcatgagga aattcttcca
     5521 gaagtaatga aactcaatcc attattgaaa gataaaatgg atgccgaaaa ttttttgaat
     5581 tctgatgaat ggatggagga aaatcctgaa tggcaagaaa tacttcaaga aagtggactt
     5641 tctgataaat tgcaggaatt aagcgatcta cagcttgaag gtgcagatgt atatatgagc
     5701 actttctcga tgttgaaatc tttccccttt ttttctgaat tttatcattg gtttttgccc
     5761 tttgatcctg aaaattctgc tataaatgaa ttatttaagg aagaagaaaa aactttactt
     5821 tcggcttttg taaatagtaa tatcatgtgc aattccgata aatattcttt ttgcctgagt
     5881 attttacaaa tgccagaatc acaaagaaat atgctcaaac aatcctttaa acttgagtcg
     5941 aatcagttag aagaaatgga aaaagaaact gctttgttat cacctgaaac ggtcgaaaaa
     6001 aatatttcca aacagtatat tcaagatttg tttcgtttct ttaaacttca tcctcaacac
     6061 aatgattttg aagatatgtt tgctacttct ttgtcgctcc atcgaactta tttatttgaa
     6121 attctggcta cagacagtaa ttttaaatta aacatcgctg aatattattt ttcaaaaggt
     6181 tatttcagac aggctttaga tttgtttgaa caacttcaat ccgaaattca accttctgcc
     6241 tccttgtatc aaaaaatggg atattcctat cagcagactt ctcaattaca aaaggcattg
     6301 gaagcttatt tgaaggctga tataattcaa cctgatgatg aatggaccat tcgtaaaatt
     6361 gccttatgtt atcgtttgct gggtaatcaa gaaaaagctt tacaatttta tcaatatgca
     6421 gattttttaa atcctaatca atataccatt caaaggcaca ttgcacaatg ttacgtagag
     6481 ttaggtaaat atcaggaagc tttgaatatt tattataaac ttgatgctga aaatggggat
     6541 gatataaaaa tatggcgagc cattagctgg tgttcatttg taatgggaga tttgaaaaaa
     6601 gctgaatatt actcccaaaa aatacttgct tctactcctg tagcatatga ttttataaat
     6661 ggaggacata ttgcatggtc tcaaggaaaa atatctgaag ctcttgaatt atattgcaag
     6721 ggtttaaact tgttgcagaa taattgggaa gtatttcagg atactttttt tgaagataaa
     6781 tcccatttgc ttgcaagggg aattaaagaa aatgaaattc ctttaatgtt agatgaaatt
     6841 ttatat
//
LOCUS       DAOSAS010000101         6708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_7315_length_6708_cov_14.873591, whole genome shotgun sequence.
ACCESSION   DAOSAS010000101 DAOSAS010000000
VERSION     DAOSAS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7315_length_6708_cov_14.873591"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..2801
                     /gene="priA"
                     /locus_tag="PLR72_05600"
     CDS             342..2801
                     /gene="priA"
                     /locus_tag="PLR72_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOK36707.1"
                     /translation="MKYIDVILPLPLTNTFTYVVPDELDELAQPGMRVVVPFGKKKMY
                     SAIIYLVHSIKPNLPYEIKEIICFLDEKPVIRPLQIKFWEWIANYYLSSLGEVYQSAI
                     PSGLKLESETRICINSSFEIDDSFSKREMKILEALSDKKEITISQLIKKTGINNILPL
                     LNSLLEKNAIEIKEELTEKIRPKTEIFVRLSPSAQNEEQLHQIFDSLARAKKQLNLLM
                     TYVELSKCLSPQPVEVSKKMLLEKSGVSATTLDALVKRKILEIYTKEIDRLNHSALPV
                     NDLYPLNSFQQKALSEIEEQFLQKNVVLLHGVTSSGKTELYLHLIQKTLNEGKQVLYL
                     VPEIALTTQLTSRLRRVFGNKLGVYHSRFSDAERVEIWNNVLNNKGYEVIIGARSSIF
                     LPFRQLGLIIVDEEQETSYKQFDPAPRYHARNAAIVLASLHEAKTLLGTATPAIETYY
                     NAQIGKYGLVELNQRYLELSMPEILVVDLKEAYRKRQIEGHFTPLLLEKMKNALENRE
                     QIILFQNRRGYARYLECKMCAYVPKCKNCDVSLTFHKTSNTLICHYCGYSEPISTVCP
                     ACGTPHELSNKGFGTEKIEDEIRKFFPETRIARMDLDTTKSRKSYEKIIADFEQYKID
                     ILVGTQMVTKGLDFDRVSLVGILNADNLFHFPDFRAYERAFQLLAQVSGRAGRKNKQG
                     TVILQTFEPQHPIIQQVINNDYLSMYRMQCKERKQFKYPPYYRLIEISLRHRKLELLE
                     QASQQMVNELQFVFGKRVLGPNTPLISRIQNQYIKNILLKMEIGASIEKTKKLVMEVI
                     NNISSLPPYKSLRIQIDVDPL"
     gene            3110..4501
                     /locus_tag="PLR72_05605"
     CDS             3110..4501
                     /locus_tag="PLR72_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOK36708.1"
                     /translation="MKNKYVDLIEQTFEFPNEEFNVIDNELYWSGIPLMDVIEQYGTP
                     LRIAYLPKIGENIQKARRMFNVAMAKVDYNGSYYYCYCTKSSHFSFIMEEVLKHDVHL
                     EISSAFDINIMESLFERGLLSQDKYILCNGFKRPQYIENIQNLRRMGFTNIIPVLDNK
                     FELDLLLKNFDLKFKIGIRIAAEEEPKFDFYTSRLGIRYNDIIPYYLKHIANNPQVEL
                     KLLHFFINTGVKDTAYYWNELNKAVNLYCELKKKCPELDGLNIGGGFPIQTHLDMIYD
                     YEYMAEEIIAQIKNICTQNGVPEPNIFTEFGSYTVGESGAMLYSIVNQKKQNDRELWD
                     MIDSSFMTTLPDTWAINQRFIFLAINNWDSEYERVNLGGLTCDSEDFYNAEVHSNAIF
                     LPKIQEGQQQYIGFFHTGAYQEALSGYGGIQHCLIPAPKMVIIDKDEDGEYFTKLFAK
                     EQSYKSMLKILGY"
     gene            complement(4559..5716)
                     /locus_tag="PLR72_05610"
     CDS             complement(4559..5716)
                     /locus_tag="PLR72_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOK36709.1"
                     /translation="MNPNFDKIRCYNDEEVHDVLERLCNEKQFMKIMSTVYPFLPKEM
                     IKKRLLSIHTQLDFQKEIILPFVQYLVANVTTNGIALYGLEKIMKEETYLYLSNHRDI
                     VSDPVFLCYKFLENGFDTVEVAIGDNLLIYPWIEDLVRLNKSFIVKRGLTTHQMLESS
                     KLLSEYIWYTLHEKKHSIWMAQREGRAKDSNDKTQESLLKMLNLAGKGSFIENMMELN
                     ICPLSISYEYDPCDYLKAKEFQLRRDSDYKKQPADDIENMVTGILGFKGKVVFHLTGC
                     INEDLKKMENKVSNRNQQVEQVAQLIDRQIYSHYAIFSNNKIAYDALTGENRFATDYS
                     EIERIDFEQYLLKQISKIQIENKDENFLRAKMLEMYAYPLINYLKAIGEDN"
     gene            complement(5769..6593)
                     /locus_tag="PLR72_05615"
     CDS             complement(5769..6593)
                     /locus_tag="PLR72_05615"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOK36710.1"
                     /translation="MEKITNEFLKRSISAVVYVLLVVASLFISPATFVIVFAFFMSMC
                     IYEFHKITNQQPEVNVPRWLSMGISLLLFLISFFYFSGRAFLPIFSYYIILIFWMFIR
                     ELFLKNPNPVHNWAYFLLGQVWIALPFSLLNFIVYFNNYQPLLILSIFILVWMNDTGA
                     YLIGITLGKHKMIPRVSPHKSWEGFAGGILVTLLTGYLLSLWLYQLDLPQWLFMSLIV
                     SIFATFGDLLESLWKRTFQIKDSGNAIPGHGGFLDRFDSLLMVVPAVLIYLIFLFN"
BASE COUNT         2333 a         1120 c         1085 g         2170 t
ORIGIN      
        1 cagataattc tatgacttct ctgcagaaat ccttgaaaat aaatatttta ttacactgaa
       61 aactcagaaa tttttctatt acttctacat tcagccgttg tatgaaaagt ctttacttaa
      121 aacaacaagt gcctgactat caactaaata ttcaaaaccc caaactcggg actcaaaact
      181 cgcatctcgc acctcaaaac tcttcaataa atttttgaaa aacataactt tctcacgaaa
      241 ttgacacatt tttcaaatag ataaattatt tttgtattat caacgcttaa gaaagatatt
      301 ttaaaaattc tttcctttat ttttagtaaa aaattctgag attgaaatac attgatgtta
      361 tattgccttt gccgctgacc aatactttta cctatgtggt tcctgatgaa ttggatgagt
      421 tggctcaacc aggtatgcga gtagtagtgc cgtttggcaa gaaaaaaatg tattcggcta
      481 ttatttattt ggtacatagc ataaagccca atttacctta tgagattaag gaaataattt
      541 gttttctgga tgaaaaacca gtaattcgac cgctgcaaat taaattttgg gaatggattg
      601 ccaattatta tctgtcttca ttaggtgaag tgtatcaatc agcaatacct tcaggactga
      661 aactggaaag cgaaacacgt atttgtatca attcttcttt tgaaatagat gattcttttt
      721 caaaacggga aatgaaaatt ttagaggcac tttctgataa aaaagaaatc actatttctc
      781 aactcataaa aaaaacaggt atcaataata ttcttccttt gcttaattca ttattagaga
      841 aaaatgctat tgaaataaaa gaagaattga ccgaaaaaat tcgtcccaaa acggagattt
      901 ttgtccgatt gagtccatca gctcaaaatg aagaacaatt acaccagatt tttgattcgc
      961 ttgcccgtgc aaagaagcaa ttgaatttat taatgactta tgtggaactt tcaaaatgtt
     1021 tgtcgccgca gccggtcgaa gtttcaaaaa aaatgttact ggaaaaatca ggtgtctctg
     1081 ctactacttt ggatgcattg gtcaaacgaa agatattaga aatctacacc aaagaaattg
     1141 accgattgaa tcacagtgct ttgccagtga atgatttgta tccattgaat agttttcagc
     1201 aaaaagcttt gtcggaaatt gaagagcaat ttcttcaaaa aaatgtggtt ttactacacg
     1261 gagtaacttc cagtggaaag acagaacttt atcttcattt aatccagaaa acacttaacg
     1321 aaggcaaaca agtgctttat cttgttccag aaatagcttt aactactcaa cttacttctc
     1381 gactgaggcg tgtttttggt aataaattgg gagtttatca ttccagattt tctgatgctg
     1441 aacgtgttga aatttggaat aatgttttga acaacaaagg atatgaagtc attattgggg
     1501 ctcgttcttc tatttttctt cctttccgac aattaggatt aatcattgtg gacgaagagc
     1561 aggaaaccag ctacaaacaa tttgatccag ctcctcgcta tcatgcacgc aatgcagcta
     1621 ttgttctggc ttccttgcac gaggcaaaaa ctcttttagg aacagctact cctgccatcg
     1681 aaacttatta taatgctcaa attggaaaat atggactggt agagctgaat caacgttatc
     1741 ttgaactttc catgcctgaa attttagttg tcgatttgaa ggaagcatac cgcaagaggc
     1801 aaatagaagg acactttaca ccattgttgc tggaaaaaat gaaaaacgcc cttgaaaata
     1861 gggaacaaat catattattc caaaatcgac gtggttatgc acgttatctt gaatgtaaaa
     1921 tgtgtgctta tgttccaaaa tgtaaaaact gtgatgtaag ccttaccttt cataaaactt
     1981 caaacacgct aatttgtcat tattgtggat acagtgagcc tatttcgaca gtttgtccag
     2041 cttgcggaac gccacatgaa ctttccaaca aaggttttgg tacagaaaaa attgaagatg
     2101 aaatcagaaa attttttcct gaaactcgca ttgccaggat ggacctggat actaccaaaa
     2161 gtagaaaatc ctatgaaaaa attattgctg actttgaaca gtataaaatt gacatacttg
     2221 ttggcactca aatggttaca aagggtttgg atttcgatag agtaagtttg gtaggaattc
     2281 tgaatgcaga taatcttttt cactttccag atttcagagc ttatgaaaga gctttccagc
     2341 tactggcaca ggtgagtggt agagcaggaa gaaaaaacaa acaaggcacc gtcatattgc
     2401 agacttttga acctcaacat ccaattattc agcaagtgat caacaacgat tatttgtcaa
     2461 tgtaccgtat gcaatgtaaa gaacgaaaac aattcaaata tcctccctat tatcgattga
     2521 tagaaatttc acttcgccac cgaaagctgg aattattaga acaagcctcc caacaaatgg
     2581 taaatgaatt acaatttgtg tttggcaaac gtgttttagg tcccaatact cctctcattt
     2641 ctcgcattca gaatcagtat atcaaaaaca ttctgcttaa gatggaaatt ggtgcttcca
     2701 ttgaaaaaac caagaaattg gttatggaag ttattaataa tatttcttca cttcctcctt
     2761 acaaatcgtt acgtattcaa atagatgtag atcctttatg acaaagtaag ttagttggat
     2821 gtgtatttat ttgacaatct atcttgtcta attttatcca tttattcata aagaaatgag
     2881 taaaaacctg ttttatttcc agccactttt tacagttttt taaattttta atataatctt
     2941 ctgatttgca gccatttaat aaaattaaat attttctatt ttattgttgg caattaaaat
     3001 taatatatta tttttgcacg tttttatgaa agatcgaaat ctattttagt tagataagaa
     3061 aatttcaatc aatctaattt acttacatta aatagaaaag gaaaaaagaa tgaaaaataa
     3121 gtatgttgat ctcattgaac aaacttttga gttccctaac gaagaattca atgttattga
     3181 caatgaatta tattggagcg gcattccttt gatggatgtg atagagcaat atggtactcc
     3241 attaagaatt gcttaccttc caaaaattgg agaaaacatt cagaaagctc gtcgaatgtt
     3301 taatgttgct atggccaaag tggactataa tggtagttat tattattgtt actgtaccaa
     3361 aagttctcat ttttctttta ttatggaaga agtgttgaag cacgacgttc atttggaaat
     3421 atcctcagct tttgatatca atattatgga atcattgttt gaaaggggat tactttcaca
     3481 agataagtat attctttgca atggctttaa aagacctcaa tatatagaaa atattcaaaa
     3541 tttaaggcgg atgggattta ctaatattat tcctgttctg gataataaat ttgaattgga
     3601 tttattgctg aaaaactttg atctgaagtt caaaattggt attcggattg ctgctgaaga
     3661 ggaaccgaaa tttgattttt atacttcacg tttaggaatt cgatacaatg acattattcc
     3721 ttattatttg aaacatattg ccaacaatcc gcaagtagag ctgaaacttt tgcatttctt
     3781 tatcaacaca ggtgttaaag atacagccta ttactggaac gaattaaata aagctgtaaa
     3841 tctctactgt gaattaaaga aaaaatgtcc tgagcttgat ggtcttaaca ttggtggagg
     3901 atttcctatt caaacacatc tggacatgat ttatgattat gaatatatgg cggaagaaat
     3961 tatagctcaa ataaaaaata tttgtacgca aaatggtgtg ccagagccaa atatttttac
     4021 tgaatttggt tcttatacgg ttggcgaaag tggtgcaatg ctatattcta ttgtgaatca
     4081 aaaaaaacaa aacgatcgtg aattatggga tatgattgac agttcgttta tgacaacctt
     4141 accagacacc tgggctatta atcaacgatt tatatttctg gctatcaaca attgggattc
     4201 agaatatgaa cgcgtaaatt tgggaggatt gacctgcgac agtgaagatt tctataatgc
     4261 tgaagttcac tccaatgcta tctttttacc taaaattcaa gaagggcagc aacaatacat
     4321 tggatttttc catacaggcg cttaccaaga agctttgagt ggttatggcg gaattcaaca
     4381 ttgcctcatt cctgcaccca aaatggtgat tattgacaaa gatgaagatg gtgaatattt
     4441 caccaaactt tttgcaaaag agcaaagtta taagtcaatg ctaaaaattt tgggatatta
     4501 ataaaaacgc ctcatttttc gatttctcga atgaggcgtt ttttttataa ataaattttc
     4561 aattatcctc tcctatagct ttcaaataat ttatcaaagg ataagcatac atttccagca
     4621 tttttgctct aagaaagttt tcatctttat tttctatctg aatttttgaa atttgtttca
     4681 ggagatactg ttcaaaatca attctttcta tttctgaata atctgttgca aaacgatttt
     4741 cgcccgtcaa ggcatcatac gcaatcttgt tattggagaa aatagcataa tgggaataaa
     4801 tttgtctgtc aatcaattga gccacctgtt caacttgctg attgcgattg ctgactttat
     4861 tttccatctt ctttaaatct tcatttatgc aacctgtcaa gtgaaaaact acttttcctt
     4921 tgaagcccag aatgcccgtt accatatttt caatatcatc agcaggctgt tttttataat
     4981 ctgaatctct acgtaattga aattcctttg cttttaaata atcacaaggg tcatattcat
     5041 aagaaatact taaaggacag atatttagtt ccatcatatt ttcaataaaa gaacctttcc
     5101 ctgccaaatt cagcatttta agaaggcttt cctgtgtttt atcattggag tctttcgcac
     5161 gaccttctcg ttgagccatc cagatagaat gttttttttc gtgaagtgtg taccaaatat
     5221 attcagaaag taatttcgaa ctttccagca tctgatgggt ggtcaagcca cgttttacaa
     5281 taaaactttt gttcagacgc actaaatctt caatccaggg ataaatcaac aaattatcac
     5341 caatagccac ttcaacggta tcgaaaccat tttccaaaaa tttataacaa agaaacaccg
     5401 gatcggaaac aatatctcga tgattggaaa gataaaggta agtctcttcc ttcatgattt
     5461 tttcaagtcc atacaaagct attccattgg ttgttacatt agcaacaaga tattgaacaa
     5521 aaggtaaaat aatctctttc tgaaaatcca attgagtatg aatgcttaac agacgttttt
     5581 ttatcatttc cttaggcagg aacggataaa cagtgctcat tattttcata aactgttttt
     5641 cgttacataa ccgttctaaa acatcatgaa cttcctcgtc attgtaacaa cgtattttat
     5701 caaaattagg attcattttt cactgaattt taagaaaaat tttatttaat tcaattatta
     5761 acaagtaatt agttaaataa aaaaatcaaa taaataagaa cagcaggaac caccataagc
     5821 aaactgtcaa agcgatctaa aaagcctcca tgtcctggaa tggcgtttcc actatcttta
     5881 atttgaaacg ttcttttcca gagtgattcc agcaaatcgc caaaagttgc aaaaatacta
     5941 actattaaag acataaatag ccattgtggc aaatcaagtt ggtaaagcca caaagaaagc
     6001 aaataacctg ttaataaagt tacaagaata cctcctgcaa aaccttccca tgatttatga
     6061 ggtgaaacgc gtggaatcat tttgtgtttc cccaaagtta tgccgattaa ataagctcct
     6121 gtatcattca tccagacaag aataaaaatt gaaagaatta aaagcggttg ataattatta
     6181 aaatacacaa taaaattaag aagcgaaaat ggcaacgcaa tccaaacctg tcctaacaaa
     6241 aaataagccc aattatgaac aggattggga tttttcaaaa ataattctct gataaacatc
     6301 cagaagatga gaataatata ataagaaaaa ataggaagga aagctcttcc agaaaaataa
     6361 aagaaagaaa tcaaaaaaag cagaagactg attcccattg aaagccagcg tggcacattt
     6421 acttctggtt gttgattggt aattttatga aattcataaa tacacataga cataaaaaag
     6481 gcaaaaacaa ttacaaaagt tgcggggctg ataaaaaggc ttgctacgac caacagtaca
     6541 taaacaacag cactgatgga gcgttttaaa aattcatttg ttatcttttc cattgttaat
     6601 tttataaaaa ttcagaatta aataacctcg aggtatcttg ttagtcgaac aatgcaaatt
     6661 gcccatcata caatttttta tatttcttct catttccctg atttgaaa
//
LOCUS       DAOSAS010000102         6699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_7327_length_6699_cov_15.022125, whole genome shotgun sequence.
ACCESSION   DAOSAS010000102 DAOSAS010000000
VERSION     DAOSAS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7327_length_6699_cov_15.022125"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2127
                     /locus_tag="PLR72_05620"
     CDS             <1..2127
                     /locus_tag="PLR72_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOK36711.1"
                     /translation="APYVFEKIGTRLEHFMIDEFQDTSVLQWKNFYPLILNSLSEGKF
                     NLVVGDVKQSIYRWRNSDWKILDEQIYNDFSSEQLHEENLATNWRSDKNIVEFNNAFF
                     FQAARLLQSKLNEAMKNALSQYNELNKFITKIEHAYSNVYQQISPKTGLGHIKFTFID
                     EEENEEGWQAESLNRLPSLLEDLQERGYQPKDIAILVRKNEEAQAVVEKLLNYKTTPE
                     AKENCCYDVLGNEGLLIGNSGSVHFILGILYLFINQKDSIQQTIVNYEYAKRGLKKSE
                     NEALQLSFSNGEFDEDVFSPLFTREENDQLKYLQHCSLYDMVEGIISLFEVGNWQNEA
                     VFVQAFQDLVFDFSVNKTADLNSFLEWWEETSPKQFIPSPENQNAIRIMTIHKAKGLA
                     FKVVILPFCDWNMDTRQRNILWCEPQISPFNEFPLLPIEYTSKLENSIFTVDYFNEQL
                     HLYIDNLNIAYVAFTRAKNELIGFCKMPKKNSKVTSLSTLLMISFENVAENSELNAHF
                     SSETNTFEWGEPTFANRELENENFTSEKINYYPSVSSKDRLKIRSQNFIQFLDEQHLT
                     DTRLNYGIVMHEVLRQMITRNDQEKAIQKMIMMGAITVEESKIVEQELESFWNLPETK
                     EWFGANVKIMNEVTILTPSGEQFRPDRVVIHGNEAMVVDYKFGEKESENHLRQVKRYM
                     NLISQMGYAVKGYVCYVSLGKVMEVN"
     gene            complement(2167..2847)
                     /locus_tag="PLR72_05625"
     CDS             complement(2167..2847)
                     /locus_tag="PLR72_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOK36712.1"
                     /translation="MKKQVSLSFMFCSLLFTVCLIVANIVEQKLISIGPLEATAGLLI
                     FPVSYIINDLIAEVWGYKKARLIIWGGFAMNFLAVLIFKLSIIVPGSENFSHQEAFAL
                     VLNNTQRITFASFLAFLVGSFLNAYVMSKMKILQHGRNFSLRAVASTLVGESGDSLVF
                     FSIAFGGIISLHDLLLLIVTQTTMKTLYEILVLPLTNVIVKWIKKKENLDTYDYQISY
                     NPLKVREI"
     gene            complement(3303..3839)
                     /gene="ribH"
                     /locus_tag="PLR72_05630"
     CDS             complement(3303..3839)
                     /gene="ribH"
                     /locus_tag="PLR72_05630"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOK36713.1"
                     /translation="MTTQYKNLSSYNEDEMPDKKKVFTQQYAIVVADWNPQVTQSLLQ
                     GTYDALIENGANPDLITIIHVPGAFELIFAAKQFMDEYSEIDNIKVHKYAAIIALGCV
                     IQGETPHFAYVCQGVTYGIATLNANSNGCPVIFGILTTQNLQQALDRAGGLHGNKGFE
                     AGITAIKMANIDWHTSIF"
     gene            complement(3892..4566)
                     /locus_tag="PLR72_05635"
     CDS             complement(3892..4566)
                     /locus_tag="PLR72_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK36714.1"
                     /translation="MSKKEKKKDEFKNVEGALTASEIFIEKYQKQILYVVGGIVLVVL
                     LLLAARNFYIQPREKAAENEMYKAQYYFSVDSFQVALEGKEPEFIGFKQIVSEYGFTN
                     SGKLAAAYAGICYYKLGDYKNAIKYLAQFDKKDTYFTTAVLGLIGDSYVELGEKDKAL
                     GYFEKAIATKNNVLSPVYLKKAGLLCESINQLQKAEKYYMEIKENYPKSMEATDIDKY
                     LARVQK"
     gene            4803..5759
                     /gene="fmt"
                     /locus_tag="PLR72_05640"
     CDS             4803..5759
                     /gene="fmt"
                     /locus_tag="PLR72_05640"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOK36715.1"
                     /translation="MGKTKEELRIVFMGTPEFAVESLKTLVENGYNVVGVVTMPDKPA
                     GRGYKVQFSPIKQYALTKNLNLLQPEKLKDETFLENLRALKADLQIVVAFRMLPEVVW
                     NMPPLGTFNLHASLLPQYRGAAPINWAIINGEKETGVTTFFLTHEIDTGKIILQEKIP
                     ILPSDNAGTLHDKLMGLGAELVKKTVDMIISDKIQTIEQSQLVKNNIELKSAPKIFKE
                     TCEIDISWNVERAHNFVRGLSPYPGAWLGINFPNSPERIILKIFRTEPEFSSHQLPLG
                     KVVTDGKKFAKIALKDGFLVLKEVQAPNKKRMEIGEFLRGLQ"
     gene            complement(5765..6142)
                     /locus_tag="PLR72_05645"
     CDS             complement(5765..6142)
                     /locus_tag="PLR72_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOK36716.1"
                     /translation="MKKIINTHNAPAAIGPYNQAIEINNMLFISGQIPISPKEGKIVS
                     LTILEQTEQIFSNIEAILKFAGYSFENVVKTTVFLTDINHFAAMNEVYKKYFTNNCPA
                     RSTVAVKALPHGALVEIETIAIK"
BASE COUNT         2346 a         1013 c         1082 g         2258 t
ORIGIN      
        1 gctccttatg tatttgaaaa aattggtaca aggctggagc attttatgat tgatgagttt
       61 caggatacct ctgttttgca atggaaaaat ttttatccac tgattttaaa tagtttatca
      121 gaaggaaaat ttaatctggt ggtgggggat gtgaaacaaa gtatttaccg ctggcgcaat
      181 tcagactgga aaattcttga tgaacaaatt tacaatgatt tttcatcaga acaattgcat
      241 gaagaaaatt tggcaactaa ttggcgaagc gacaaaaata ttgtagaatt taacaatgcc
      301 tttttctttc aagctgcacg tttactccaa tcaaaactaa atgaggcaat gaaaaatgct
      361 ttatcacagt acaatgaatt aaataaattc atcactaaaa ttgaacatgc ctattcaaat
      421 gtttatcaac aaatttctcc aaaaacaggg ttaggacata taaaattcac ttttattgat
      481 gaagaagaaa atgaagaggg ctggcaagct gaaagtttga atagattgcc ttctttgttg
      541 gaagatttac aggaaagagg ttatcaacca aaagatatag ccattttggt acgaaaaaat
      601 gaggaagctc aagcggtagt tgaaaaacta ctgaattaca aaaccactcc tgaagcaaaa
      661 gaaaattgtt gttatgatgt gctggggaat gaaggattat tgattgggaa ttcaggcagt
      721 gtacatttta ttttgggaat tttgtatctt tttataaatc agaaagatag cattcagcaa
      781 accattgtaa attatgaata cgcgaaaaga ggattaaaaa agagtgaaaa tgaagccctt
      841 caactcagtt tttctaatgg tgagtttgat gaagatgtgt tttctccttt gtttacaagg
      901 gaagaaaacg atcaattgaa atatcttcaa cattgttctc tttacgatat ggtggaagga
      961 attatttccc tttttgaagt aggcaattgg caaaatgaag cagtttttgt tcaagcattt
     1021 caggacttgg tatttgattt ttctgtcaat aaaacggctg atttgaacag ttttttggaa
     1081 tggtgggaag aaaccagtcc taaacaattt attccatcgc ctgaaaatca gaatgcaatt
     1141 cgtatcatga ccattcataa agccaaaggg ctggctttta aagtggtgat tctccctttt
     1201 tgcgactgga acatggatac acgacaacgg aacattttat ggtgcgagcc ccaaatttct
     1261 ccttttaatg aatttccttt attgccaatt gaatatactt caaaactgga aaattccatt
     1321 tttacagtag attattttaa tgagcaattg catctttaca ttgataatct aaacattgct
     1381 tatgtagctt ttacacgagc taaaaacgaa ttgatcggtt tttgtaaaat gcctaaaaaa
     1441 aatagtaagg ttacttcttt atcaacttta ctaatgataa gttttgaaaa tgttgccgaa
     1501 aattctgaat taaatgctca tttttcttca gaaaccaata catttgaatg gggcgaacca
     1561 acttttgcta atagagagct tgaaaatgag aattttacaa gcgaaaaaat aaattactat
     1621 ccttccgtga gcagtaaaga ccgtttgaaa attcgttcac aaaatttcat tcaattctta
     1681 gacgaacaac acttaacgga tactcgtttg aattatggta ttgtgatgca tgaagtgtta
     1741 cgccagatga tcactagaaa tgaccaagaa aaagcgatac aaaaaatgat aatgatggga
     1801 gctattacgg ttgaggaaag taaaattgta gaacaagaac ttgaatcttt ctggaatttg
     1861 cctgaaacaa aagaatggtt tggagcaaat gtaaaaataa tgaatgaagt tactattctt
     1921 actccatcag gcgaacaatt tcgtcccgac cgtgtcgtaa ttcatggaaa cgaagcaatg
     1981 gtagttgatt ataaattcgg agaaaaagaa tcagagaatc atttacggca agtgaaacgg
     2041 tacatgaact tgatttccca aatgggttat gcagtaaaag gctatgtttg ttatgttagt
     2101 ttaggcaaag taatggaagt caattagatg aaaaattaac tctttggagt taaaccaaaa
     2161 tagtagctaa atttctctta cttttaaagg attatacgaa atttgataat cataagtatc
     2221 gagattttct tttttcttta tccattttac aatcacattt gttaaaggta aaaccagaat
     2281 ttcataaagg gttttcatgg ttgtttgtgt aacaattaaa agcaacaaat cgtgaagaga
     2341 aataatgcca ccgaaagcga ttgaaaaaaa caccaatgaa tcaccacttt cgccaaccag
     2401 ggtggaagct acggcacgta aagaaaaatt tcgtccatgt tgaagaattt tcattttgct
     2461 cataacatag gcattcaaaa aagagccaac aagaaaagca agaaaacttg caaaagtaat
     2521 acgctgagta ttgttcaaca ccaaagcaaa agcttcttga tgagaaaaat tttcagagcc
     2581 aggaacgata atactcaatt taaaaatcaa tactgccaga aaattcatgg cgaaaccacc
     2641 ccagataatg agacgagctt ttttataacc ccatacttcg gcaattaaat catttataat
     2701 gtaagaaaca gggaaaataa gtaagcctgc tgtggcttca agaggtccga tagaaataag
     2761 tttttgttca acgatatttg ctactatcaa gcagacggta aaaagtagcg aacaaaacat
     2821 aaaagagaga gaaacttgtt ttttcatttt cttgtttttt acgtggtttt caagcacacg
     2881 ttttgcaaaa aatagtgcaa atgtaattgt ttttttctcg aatagaagga aaaaatagga
     2941 gtttttctcc tgctttattt ccgatctctc tcccactatc gacatcagtt gaaatatggt
     3001 ggttcgttaa aaagattgag aattgcagct tttttaattg gtaacataca taaaaaaggg
     3061 ttcaccatct tttattggat agtttttatt cattgttatt tgtacaatat gaaactatga
     3121 gctactccac cattttcgtt gctgctcttt ggataccaat aaaattattt ttatcccaag
     3181 aattaacaaa taaaactcac actcatcaaa aagcaatcat aaattaccaa tagtaagtag
     3241 gaatcttctt attccataat taatttcatt ttcacttatc tgctcagtat aagctttaat
     3301 ccttagaaga tactggtgtg ccaatcaatg tttgccatct taatagctgt aattccagct
     3361 tcgaatcctt tattgccatg taagccgcct gctctgtcaa gtgcttgttg taaattttga
     3421 gtggtaagta ttccaaagat gacagggcat ccatttgaat tcgcatttaa tgtggcaatt
     3481 ccataagtaa ctccttgaca aacataagcg aaatgaggtg tttctccttg aatcacacaa
     3541 cctaaggcaa taatagcagc atatttatga acttttatat tgtctatttc agaatattca
     3601 tccataaatt gtttggctgc aaagatgagc tcaaaagcgc cgggtacatg gataatagta
     3661 attaaatcgg ggttggctcc gttttcaatt aaagcatcat aagtaccttg cagtaatgat
     3721 tgtgtcactt gaggattcca atcagctact acaatagcat attgttgtgt aaacaccttt
     3781 tttttatcag gcatctcatc ttcattatag ctggataaat ttttatattg tgttgtcatt
     3841 tttatttttt atttttaaaa atattttttt taatgaagat tataaatttg tttacttttg
     3901 aacacgggca agatatttat caatatcggt ggcttccata ctttttggat aattttcttt
     3961 tatttccata tagtattttt ctgctttttg aagttggttg atcgattcac ataataaacc
     4021 tgcctttttt aaatatacag gacttaaaac attgtttttt gttgcaatag ctttctcaaa
     4081 gtatcctaaa gctttatctt tttctcctaa ttctacataa ctatctccaa ttaagccaag
     4141 tactgctgtt gtaaaataag tgtctttttt gtcaaattgg gcaagatatt taatagcatt
     4201 tttgtaatca cccaatttgt aataacaaat acctgcataa gcagcagcga gtttacctga
     4261 atttgtaaag ccgtattccg aaacgatttg cttaaatcca ataaattctg gctctttccc
     4321 ttctaatgct acttgaaatg aatcaacact aaaataatat tgagccttat acatttcatt
     4381 ttcagccgct ttttctcgtg gttgaatata aaaatttcga gctgccaata atagcaaaac
     4441 aacgagaaca attcctccaa ctacatataa tatttgtttc tgatactttt ctataaaaat
     4501 ttctgaagcc gttaaagctc cttctacatt tttaaattcg tctttctttt tttctttttt
     4561 tgacataaaa atggtcttct tttagtttct taaattgaag attgtttaaa ttaatgagca
     4621 acaaaattaa gatttttctt ttagataatg aaattttagg ttaagttttt aaaacttcca
     4681 attattcgca taaaataaat aaattgtaga aaaactgttt tatgctttat tcataagagt
     4741 tggtagattt ttttattatt tttgtcgcaa aagaatgact atcttaaaaa agattagaaa
     4801 atatgggaaa aacaaaagaa gaattacgaa ttgtttttat gggaacacca gaattcgctg
     4861 ttgaaagttt aaaaacatta gttgaaaacg gttataatgt ggttggcgtg gttactatgc
     4921 ctgataagcc tgctggcaga ggctataaag ttcagttctc accaataaaa caatatgctt
     4981 taacgaagaa cttgaacttg cttcagcccg aaaaattgaa agacgaaact tttttagaaa
     5041 atctacgagc actgaaagca gatttacaaa ttgtggtagc ctttcgcatg ttacctgaag
     5101 tagtttggaa catgccccca ttgggaactt tcaatcttca tgcttcctta cttccacaat
     5161 atagaggtgc agctcctatt aactgggcaa ttatcaatgg tgaaaaagaa actggtgtaa
     5221 caactttctt tctaacccac gaaattgata ccggtaaaat tattcttcag gaaaaaattc
     5281 ccattttacc ttctgataat gctggtactt tgcacgataa attaatggga ttgggtgcag
     5341 aattggttaa aaaaacggta gatatgatta tttcagataa aattcaaaca atagaacaat
     5401 ctcaattagt gaaaaataac atagaattga aatcggctcc aaaaatcttc aaagaaactt
     5461 gtgaaattga tatttcctgg aacgttgaac gtgctcataa ctttgtacga gggctttctc
     5521 cttatccggg agcttggttg gggataaatt ttccaaattc tcctgaaaga ataattttaa
     5581 agatttttcg aacagaacca gaattttctt ctcatcaatt accattagga aaagtagtaa
     5641 ccgacgggaa aaaatttgcc aaaatagcct taaaggatgg ttttttagtt ttgaaagaag
     5701 tgcaagcccc caacaaaaag cgaatggaaa ttggcgaatt tctacgaggt ttgcagtaaa
     5761 tttttcattt tatagctatt gtttcaattt ccaccaaagc tccgtgcgga agtgctttta
     5821 cggcaaccgt tgaacgagca ggacaattat tggtgaaata ctttttatat acttcattca
     5881 tagccgcaaa atggtttatg tccgttagaa aaaccgttgt tttgactaca ttttcaaaag
     5941 aataaccagc aaactttaaa atagcttcaa tattggaaaa aatttgttct gtttgttcta
     6001 aaatagttaa tgaaacgatt tttccttctt ttggcgaaat aggaatttgt cccgaaataa
     6061 aaagcatatt atttatttct atagcctgat tataaggtcc aatagctgcc ggagcgttgt
     6121 gagtattgat aatttttttc atagtaataa aaaatttaga ataaacactt taaataaacc
     6181 atgtttaatt tttttaatat aaattttccc atcatatttc catagtttag cttcatcaat
     6241 aattacaaaa ataactaaaa tagtaatatt caataactta taaattacaa aattattaaa
     6301 attgactttt ggaagtgggt ttttataaat aattattcat ttttgaattt atacctcaac
     6361 tctgtcaata tatagtgttt tttagttgaa aattttccaa aaagggttga gaaaaaaaca
     6421 aaatattcaa gtaccaacta taaaaactat ttttatataa caattattat gaaataaaaa
     6481 aaaattatta tttctttttt taattattta ctaactaatt ttttcttttg caaagaaaag
     6541 aaatttttaa ctttaagttt ttaaatagat tgttaataac aaataaaact ttttgaaaat
     6601 aaaataaata cacttttctt ttctgttgaa aaaccaatat aaagagttga aaaaaaatat
     6661 tttacttttt aaaaaaaatt tatccacttt ttcaacaac
//
LOCUS       DAOSAS010000103         6640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_7414_length_6640_cov_12.635839, whole genome shotgun sequence.
ACCESSION   DAOSAS010000103 DAOSAS010000000
VERSION     DAOSAS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7414_length_6640_cov_12.635839"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLR72_05650"
     CDS             complement(<1..308)
                     /locus_tag="PLR72_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36717.1"
                     /translation="MKKINIFSVLIILIAFAIVSCNSNKPAKPVLKNQLDSLNYAFGV
                     ANGDGIKNYYLQGDSSDETIKSLIKGMNDGLKSKVEKEHLEISFLGTQIGSSLKEQEK
                     "
     gene            complement(335..856)
                     /locus_tag="PLR72_05655"
     CDS             complement(335..856)
                     /locus_tag="PLR72_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HOK36718.1"
                     /translation="MKKLSILSLIVVMTTFLMVSCKNKYEAKKSELSNQNDSLNYTLG
                     LVNGEGIKNYYLQNDSSDKPIEAFMNALDKAYKSKKTDKWYQLGYQIGTSLKFQESHG
                     LMADTTLAFNIKLVKQGIINALYNFDGGMSPQQAQEYLQKTMYELQAKKASEQALPSV
                     PKKDSTLDTLNTK"
     gene            complement(880..1614)
                     /locus_tag="PLR72_05660"
     CDS             complement(880..1614)
                     /locus_tag="PLR72_05660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOK36719.1"
                     /translation="MKKTIILFFSITLLIISAEAKKPKKTERKNQSDITFTNNVDSMS
                     YALGLLVGNDFLKNIKTIPGGKTNVDLIIKGFATAMKGDSAIMSFDSANNYLRDYFTA
                     AQAKDSEERKTAAIAFMEQNKKKEGIITTESGLQYEILKATEGPKPANTDKVKVNYEG
                     FLIDGTKFDSSIDRGEPIVFALNQVIPGWSEGLCLMSVGSKYKFYIPYELAYGAQGAG
                     NVIPPFSPLIFEVELLEINPDEENNQ"
     gene            complement(1726..3510)
                     /locus_tag="PLR72_05665"
     CDS             complement(1726..3510)
                     /locus_tag="PLR72_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOK36720.1"
                     /translation="MRKLSLLFILFSPLLSFGQLIENFSDGDFTNNPTWTGNISNFFI
                     NNSFQLQSKAPMTSTSFLFTPSETIDDAIWEVWVKITYTTSSSNYASVYIVSNTNDIT
                     DGCNGYFVKIGDTKDNVSLWLQEGIKKTKLIDGVEKRTDGNPVEIKIKVTRDENGNFS
                     LYSKLPSENDYFLEGTVQNLAIKQSLYFGLMYANTSTTGSAYYFDDILVRGNKAVDME
                     SPQWEQLTLLPPNQLKLQFSEPMDFLQATFTVNEEIGTPSNIEISVDKTVVILTFENE
                     FQRGKIYTLEINEATDLAGNPLLNTTKITGITEPIEMGDLVFNEIMFDNPLNSTEYLE
                     IFNRSEKLLDVSGIVFTTRKKDGNLNTGHEIPKGTLLKPAGYLAVCPNADSLLNYYHY
                     PPESYVVTTSWSNLNNESATLVLANNNKDTIYDELTYNVKWHHIMIKNPKGVSLEKIN
                     PSLPSQDPQSWHSAAFEVNYGTPGYKNSQYREIDISMEPERFIWADPEAFSPDNDGVE
                     DVCLIHYKTATNGFVATATIFNAVGVKVCQLLSNVLLASEGFFLWNGKTSDGKIANPG
                     IYVLYFEMIQPDLGIKKQAKIPIVVSLR"
     gene            3637..4644
                     /locus_tag="PLR72_05670"
     CDS             3637..4644
                     /locus_tag="PLR72_05670"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOK36721.1"
                     /translation="MRVAIVGASGAVGQEFLRVLSERNFPVTELFLFGSHRSAGKEYE
                     FNGKKIPVKELKHGDDFKNIDLAFTSAGASVSKEFAKDITKFGTVMIDNSSAFRMDED
                     VPLVVPEVNASDAKSRPRGIIANPNCTTIQMVVALNAIENISHIKRVHVATYQAASGA
                     GSAAMAELELQYKQILAGESPTVNKFPYQLAFNVIPQVDVFTENGYTKEEMKMYNETR
                     KIMHSDVEVSAMCVRVPTLRAHSESIWVETERPISVEEARKAFKNAKGVIVMDNPEEK
                     VYPMPAFLSGKDEVFVGRIRKDLSCPNGITFWCVSDQIRKGAALNAVQIAEYLINEHD
                     I"
     gene            complement(5175..6623)
                     /locus_tag="PLR72_05675"
     CDS             complement(5175..6623)
                     /locus_tag="PLR72_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOK36722.1"
                     /translation="MQEIKFYKGENIPLELHKVRIVQKLHLVPIERRLEALQEGGYNT
                     FRLNTKDVFLDMLTDSGTNAMSDNQMAAMMQADDAYAGSQSFYRLLKAVQDVLDKKYY
                     LPVHQGRAAENIISYAYVKKGSIIPMNYHFTTAMAHITQYGGKIAELLYNEAYVIDSS
                     HPFKGNMNLEKLEEIIQLHGPENIPFIRMEASTNLIGGQPFSVQNLRDVRAIADKYKI
                     RLVLDASLIGENAYLVKQREEEFKDASLGEIIKTMTGLADLVYFSARKLSSSRGGGIC
                     TNSDEIYHELEALIPLFEGFLTYGGMSVREMEAMAVGLYETLDETIICQSPNFIKYLV
                     QALDEKGIPVIKPAGVLGVHLDAMQICAHIPQKEYPAGSLAAALFLISGIRGMERGTI
                     SNQRDEYGNETYADMELLRLAIPRRVFTLSQIKYVEDRVNWLYDNRELIGGLRFVYEP
                     PVLRFFMGGLEPVNDWPQKLMTKFKEDFGDSL"
BASE COUNT         2108 a         1161 c         1129 g         2242 t
ORIGIN      
        1 attttttcct gctcttttag tgaagaccca atttgagtac ctaaaaaaga aatttccaga
       61 tgttcttttt ctactttcga tttaagtcca tcattcatgc ctttgataag agattttata
      121 gtttcatcag aagagtcgcc ttgtaaataa taatttttaa taccatctcc attggcaaca
      181 ccaaaagcat agtttaaact gtcaagttgg tttttcagaa ctggtttagc tggtttgtta
      241 ctattacagg aaacaatggc aaaagcaatt agaatgatga gaaccgagaa tatattaatc
      301 tttttcattt cgaattagtt tattgttata aattttattt cgtattaaga gtatctaatg
      361 tgctatcttt tttaggaaca gatggtaaag cttgttcaga agctttttta gcctgcaatt
      421 cgtacatagt tttttgaaga tattcttgag cttgttgagg gctcattcct ccatcaaaat
      481 tgtataaagc attgataatt ccttgcttta ccagctttat attgaaagcc agagtagtgt
      541 cagccatcaa accgtgagat tcttgaaatt ttagcgaagt gccaatctga taacctaatt
      601 gataccattt gtcagttttc ttgcttttgt aagctttgtc aagagcgttc ataaatgctt
      661 cgataggttt atcggaagaa tcattttgta aatagtaatt ttttatacct tctccattca
      721 ccagaccgag tgtgtaattt aagctatcat tctgattgct caattcactt tttttggctt
      781 cgtacttatt tttacacgaa accattaaaa atgtagtcat tactactatt aaggacaaaa
      841 tacttaattt tttcattgca tcattttttg ataatgttat tattggttgt tttcttcgtc
      901 aggattaatt tcgagcaatt ctacttcaaa aattagaggt gaaaaaggag gtattacatt
      961 tccggctcct tgtgctccat aagcaagttc gtaaggaata taaaatttat attttgaacc
     1021 tactgacatt aaacaaagtc cttcactcca gcctggaatt acctgattta aagcaaaaac
     1081 tataggttct cctcgatcaa tggagctatc gaacttagta ccatctatta aaaaaccttc
     1141 ataattgact tttactttgt cagtattagc aggtttaggt ccttcagtag cttttaaaat
     1201 ttcatattgt aagccacttt ccgtagttat tattccttct tttttcttgt tttgttccat
     1261 aaaggcaatc gctgcagttt ttctctcctc agaatctttg gcttgagctg cagtaaaata
     1321 atctctcaaa tagttgttgg cagaatcaaa agacataata gctgaatcgc ctttcatagc
     1381 tgtagcaaat cctttgatta tcaaatcaac gttagtttta ccaccaggaa tagtttttat
     1441 attttttaaa aaatcgttcc ctaccagcag acctaaggca taactcattg aatctacatt
     1501 attggtaaaa gttatatctg attgattttt cctctcagtt ttcttaggtt tttttgcttc
     1561 agcggatata atcaataatg tgatagaaaa aaataaaatg atggttttct tcattgataa
     1621 aaaatctaaa tgtttttata catgttaact taaaatgaac aagtgattta taagcggtgc
     1681 aaagatagtt aatttattac acaataacat gttaatattt caaaattatc gtagtgagac
     1741 tacaatagga attttagcct gtttttttat tcctaagtca ggttgaatca tttcaaaata
     1801 aagcacataa atgcctggat tggcaatttt gccatcgctt gttttgccgt tccagagaaa
     1861 aaatccttcg gaagcgagca gtacatttga aagcaattga catactttta caccaacagc
     1921 attaaaaatt gttgcagtag caacaaaacc attcgttgct gttttgtaat gaataagaca
     1981 tacatcttcc acaccatcat tgtcaggtga aaaagcttct gggtctgccc atataaatct
     2041 ttcaggttcc attgaaatat caatttctct atattgagaa tttttataac ctggtgttcc
     2101 ataatttact tcaaaagctg ctgaatgcca tgattgtgga tcctgtgaag gaagagaggg
     2161 attaattttt tccaatgaaa caccttttgg attttttatc ataatgtgat gccattttac
     2221 attataggtc aattcatcat aaatcgtatc cttgttattg ttagctaata ccaaagtagc
     2281 actttcgttg ttaagatttg accaactggt agtaacaaca taactttcag gtggataatg
     2341 gtaataattt agcaatgaat cagcattggg acaaacagct aagtaaccag caggttttaa
     2401 aagcgttcct ttaggaattt cgtgtcctgt attaagattc ccatcttttt ttcgagtagt
     2461 aaatactata cctgaaacat ccagcaattt ttcagaacga ttgaaaattt caagatactc
     2521 cgttgaattt agaggattat caaacatgat ttcattgaaa accagatcac ccatttcaat
     2581 tggttcagtt atgcctgtta tttttgttgt atttaaaaga ggatttcctg ctaaatcagt
     2641 tgcctcgtta atttccaaag tatagatttt accacgttga aattcatttt caaaagtgag
     2701 tattacaacg gttttgtcga cagatatttc aatattagaa ggtgttccta tttcttcatt
     2761 tacagtaaaa gttgcctgca aaaaatccat cggctcagaa aactgtaatt ttaactgatt
     2821 aggaggtaaa agagtaagtt gttcccattg aggtgattcc atatcaacag ctttattgcc
     2881 cctaaccaaa atatcgtcaa aataataagc tgaaccagtt gtgctggtat tagcatacat
     2941 taatccgaaa taaagacttt gtttgatagc taaattctga acggttcctt ccaaaaagta
     3001 gtcattttca gatggcaact tgctgtatag tgaaaaatta ccattctcgt cacgagtaac
     3061 tttaattttt atttcaacag gatttccgtc tgtacgtttt tcaactccat caattaattt
     3121 agtctttttt attccctctt gtagccataa tgaaacatta tctttggtat caccaatttt
     3181 aacaaaatag ccattacagc catcggttat atcgtttgta tttgaaacaa tataaacact
     3241 tgcataattg gacgaggaag tagtataagt gattttcacc catacttccc aaatagcatc
     3301 atctatggtt tcagaaggcg tgaacaaaaa agaagtagaa gtcattggag ctttagattg
     3361 aagttggaag gaattattaa taaaaaaatt ggaaatattt cctgtccatg tcggattgtt
     3421 ggtaaaatca ccatctgaaa aattctcgat taactgacca aaacttaaaa gaggagaaaa
     3481 aagaataaaa agtaagctta attttctcat tttttagtat atttcaacga aatggactat
     3541 ttttgtaatc tgaaatttaa agataaatcg aacaaaaata ataaattatc taatcaattc
     3601 aagaaagttt tcagaaaaat aaaaaattta aaaatgatga gagtagcaat tgttggtgcc
     3661 agtggtgccg ttggacaaga atttttgcgt gtgttgtcag aacgcaattt cccggttaca
     3721 gaattattcc tttttggttc tcaccggagt gcagggaaag aatatgaatt taacgggaaa
     3781 aaaattcctg tcaaagaatt gaaacatggt gatgacttta aaaatataga tttagctttt
     3841 acttcggctg gagcaagcgt atccaaagaa ttcgctaaag atattaccaa atttggtacc
     3901 gtaatgatcg ataattcttc tgccttcagg atggatgagg atgttccttt agtagtacca
     3961 gaagtaaatg ccagcgacgc aaaaagccgt cccagaggaa ttattgccaa cccaaactgt
     4021 accactattc aaatggtagt agctttgaat gcaatagaaa acatttctca tatcaaaaga
     4081 gtacatgttg caacttatca agctgcaagt ggagctggct ccgctgctat ggctgaatta
     4141 gaattgcaat acaagcaaat tttagctggt gaatcaccca ctgtaaacaa atttccttat
     4201 caattggcat ttaatgttat accacaagtt gatgtcttta ctgagaatgg ttatactaaa
     4261 gaagaaatga aaatgtataa tgaaacgcgc aaaattatgc attctgacgt cgaagtaagc
     4321 gctatgtgtg taagagttcc aaccctgaga gctcattctg aaagcatttg ggtagaaaca
     4381 gaacgtccaa ttagtgtaga agaagctcgc aaagctttta aaaatgctaa aggagtgatt
     4441 gtgatggata atcctgagga gaaagtctat cctatgccag catttctttc tgggaaagat
     4501 gaagtatttg taggacgcat tcgtaaagat ttaagttgcc caaatggaat tactttttgg
     4561 tgtgtttccg atcaaataag gaaaggtgca gcattgaatg cggtacaaat agctgaatat
     4621 ctgattaatg agcatgatat ttaattatat gttttggaat tattcctggg actaatcgag
     4681 gaggtagagg aattgttcct ctgccttctt attttactgt cgctgctctc gtacacggcg
     4741 atttgttact aacgttacta attattcata cctcaattaa tgatttgata gacaagaaat
     4801 aatgaagttc gatttgacta tttccaccct gattatcaat atattaacaa ttatatttac
     4861 tcaaactttt ttctgtagcg aaggagcaga tatttttcaa aaccagctct gctgttttag
     4921 ggtagttcat gatcaataca aatagttcat caatatcctt ttctaatgct tcatatattt
     4981 cagttataaa aataatttcc aaagacattt gtcaagtgtt tgctgtaaac attcagtatt
     5041 cattgccctg aaaagttcac ctattgttcg taaattcaac taattaatgc aactttttca
     5101 ggggaaataa aaggttaata aaatattttt ttaagacaag aggctgtttc aaaaaataaa
     5161 acaacctatt ataattacaa actatcgcca aaatcttcct taaattttgt cataagtttt
     5221 tgtggccaat catttaccgg ctcaagacct cccataaaga aacgaagtac gggtggctca
     5281 taaacaaatc gaagtccacc gatgagctca cgattatcgt acagccaatt cacgcggtcc
     5341 tcgacatatt ttatttggga tagtgtgaac acgcgtcttg gaatagctaa acgcaacaat
     5401 tccatatcag cataggtttc attaccatat tcgtcacgct gattcgagat agttccccgt
     5461 tccattcccc gaattcctga aatcagaaaa agtgccgcgg caagtgatcc ggcaggatat
     5521 tctttttgtg gaatgtgtgc acatatctgc atcgcatcca aatgaacacc gagtacgccg
     5581 gctggtttta tgactggaat gcctttctca tcaagtgctt gtacaagata cttaatgaaa
     5641 ttaggacttt gacagataat tgtttcatcc aatgtttcat acagtccaac tgccatagct
     5701 tccatttcgc gtactgacat gccgccgtag gtgagaaatc cttcaaaaag cggaatcagc
     5761 gcttccaatt cgtgataaat ttcatcactg tttgtacata ttccaccacc gcgtgatgaa
     5821 ctgagtttac gagccgaaaa atataccaaa tcagctaaac cggtcatggt ttttattatt
     5881 tcacctaatg aagcatcttt aaattcttcc tcacgctgct tcaccagata agcattttcg
     5941 ccaatcaggc ttgcgtcgag cacaagtcga atcttatatt tgtcggctat agctctcaca
     6001 tcacgtaaat tttgtactga aaaaggttgt cctccaatca aattggtaga tgcttccatg
     6061 cgaatgaacg ggatattttc tggtccatgt agttgaataa tttcttccaa tttttcgaga
     6121 ttcatatttc ctttgaaagg atgcgaagaa tcaatcacat aagcttcatt ataaagcagt
     6181 tcggcaattt tcccaccata ttgggtgata tgtgccattg ctgtggtaaa atggtagttc
     6241 attggtatga tgcttccttt ttttacataa gcgtatgaaa taatgttttc ggctgcacga
     6301 ccttgatgaa caggtagata atatttttta tccaaaacat cctgaacagc tttcaacagc
     6361 ctgtaaaaac tctgggatcc agcataagcg tcatcagcct gcatcatagc agccatttga
     6421 ttatcgctca tagcattggt gccactatcg gttagcatat ccaaaaaaac gtctttggtg
     6481 ttcaacctga aggtgttata tcccccttcc tgcaaagctt ccaaacgtct ttcgattggc
     6541 acaagatgta atttctgcac tattcttact ttgtgtaatt cgagaggaat gttttcgcct
     6601 ttgtaaaatt tgatttcttg catgataaaa atgattaata
//
LOCUS       DAOSAS010000104         6499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_7590_length_6499_cov_12.402079, whole genome shotgun sequence.
ACCESSION   DAOSAS010000104 DAOSAS010000000
VERSION     DAOSAS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7590_length_6499_cov_12.402079"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..2036
                     /locus_tag="PLR72_05680"
     CDS             261..2036
                     /locus_tag="PLR72_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOK36723.1"
                     /translation="MKKETYAKLIAWREKHIKERQFILILSLVVGILCSLAAFILKTS
                     IHSIQTLVTESFNKENLNYLYLITPIIGIFITSLFVRNIVKDDISHGVTRVLYAISQR
                     KSILKLHNVWTSLVGSAITIGFGGSVGAEAPIVLTGSAIGSNLGKFFKMDQKTLMLLV
                     GCGAAGAIAGIFKAPIAGLVFTLEVLMLDMTMTSIVPLLISSVTATCFTYILTGDEFM
                     FQFTHYEPFNVSRIPYLILLGVICGLVSLYFTRGTYKLENFFQKIENPYKKIAIGGSI
                     LSVLIFFFPPLYGEGYDTISTLLNGDVSSVLSQSLFFGHEDNSWALISYLVLIIIFKV
                     FASISTTSAGGVGGIFAPSLFVGCITGFVVAKLLRLMGVPIPVENFALAGMAGLMSGV
                     MHAPLTGIFLIAELTGGYYLFMTLIIVSTISYLTIILFEPHSIYAMRLAQKGELLTHN
                     KDKAVLTMLKMNNVIEKDLIILHPEMTLGELVKVIAHSKRNIFPVVDSKTGVLLGVLS
                     LDEVRNIMFRRELYHRFTVGQLMVSPPAILNINMPMEKVMEIFEDTGAWNLPVVDDQK
                     RYIGFVSKSTIFNSYRHLLVHFSDE"
     gene            2126..2335
                     /gene="xseB"
                     /locus_tag="PLR72_05685"
     CDS             2126..2335
                     /gene="xseB"
                     /locus_tag="PLR72_05685"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOK36724.1"
                     /translation="MNELTYNEAINELEKILEELENNTEINFDQITQKVQRATELLEF
                     CNKKLHQIDEDMEKLLSKLDESITQ"
     gene            2763..3800
                     /gene="asnA"
                     /locus_tag="PLR72_05690"
     CDS             2763..3800
                     /gene="asnA"
                     /locus_tag="PLR72_05690"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOK36725.1"
                     /translation="MSYFIQPNNYKPILDLQQTELGIKKIKDFFQSNLSSELRLRRVT
                     APLFVLKGTGINDDLTGVERAVSFPIKDMNGSEAEVVQSLAKWKRLALAAYGIQQGYG
                     IYTDMNAIRADEELGNLHSLYVDQWDWERVISEEERNLNFLKKIVKQIYAAFLRTEYL
                     VWESFPQIEPYLPQEIVFIHAEELRQLYPHLSPKEREIEFTKIHKAVFLIGIGAPLGD
                     GKKHDGRAPDYDDWSTIAENGLPGLNGDILFWNPVLETAFEISSMGIRVDKTALLRQL
                     EMEGAQDRLQMYFHKCLLDGLLPLSIGGGIGQSRLCMFFLRKAHIGEIQSSIWPKEMQ
                     EKCAKMNIILM"
     gene            4142..4756
                     /gene="hisIE"
                     /locus_tag="PLR72_05695"
     CDS             4142..4756
                     /gene="hisIE"
                     /locus_tag="PLR72_05695"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOK36726.1"
                     /translation="MLTKSNKMEIDFKKMNGLVPAIIQDECTSKVLMLGYMNEEALAK
                     TLETGKVTFFSRSKNRLWTKGEESGNFLNLVSITPDCDNDTLLIKVHPEGPVCHTGKD
                     TCWGESNEGDFAFLKYLQEFIQKRFKEMPEGSYTTSLFKEGINRIAQKVGEETIETII
                     EAINSNKEGFIYEASDLIYHLIVLLTAQGLTLDDIGKELKRRHK"
     gene            4772..5443
                     /locus_tag="PLR72_05700"
     CDS             4772..5443
                     /locus_tag="PLR72_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK36727.1"
                     /translation="MEKNLLIKYVDVDIYQQEMLVLKNIQLEIYSGEFIYLLGKVGSG
                     KTSLLKSFYHEVPLSEGNAAVLNYDLQKIKSKEIPYLRRRIGIIFQDFQLLTDRTVYA
                     NLEFVLRATGWKEPKAIDEQINVVLEYVGMENKGYKMPHQLSGGEQQRIAIARALLNS
                     PEIILADEPTGNLDKQTGNDLVELLYNIHFSGATVIMATHNMNWVEKFPGRIIKFENE
                     MLIEA"
     gene            5744..6481
                     /gene="lptB"
                     /locus_tag="PLR72_05705"
     CDS             5744..6481
                     /gene="lptB"
                     /locus_tag="PLR72_05705"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOK36728.1"
                     /translation="MILRTEHLVKKYSKRIVVDDVSIQVGQGEIVGLLGPNGAGKTTT
                     FYMVVGLVKPLAGEIFLNEQNITNFPVYKRAQAGIGYLAQEPSVFRKMTVEDNIKSVL
                     EMTKFSKEYQKEKLETLITEFNLSHVRKNIGDRLSGGERRRTEIARCLAIEPRFILLD
                     EPFAGVDPIAVQDIQDIVWKLKDKNIGILITDHNVDETLAITDRAYMLFDGKILFQGT
                     AKELSENPIVREKYLGRDFELKKKTKV"
BASE COUNT         2186 a          951 c         1143 g         2219 t
ORIGIN      
        1 gttgatgaac tttatccaac ttctttatga cgttcttctt tgaaagtgat agtcttattt
       61 tttctatttc ctagaaaaaa tcaattttta aactgatttt tcgtgtagta ttttttgagc
      121 gtttttctct ggactgctta cctttaggat ataattctaa ggtaatctaa atactaaatt
      181 attttcatgt ttgaaaaaac aagctggcat agaataatga ttttttttat ctttgttctg
      241 ttcatttttg gaaagagcag atgaaaaaag agacttatgc gaaattgatt gcttggcggg
      301 aaaagcatat aaaagaacgt caattcattc ttattttaag tttggtagtt ggaattttgt
      361 gttcactcgc tgcttttatt cttaaaactt ctattcattc gatacaaacc ctcgttacag
      421 aaagttttaa caaagaaaat ctcaattatt tatatttaat tactcccatc attggcattt
      481 ttatcacttc tttgtttgtc agaaacatcg taaaagacga cattagtcat ggcgtaacac
      541 gagtacttta tgccatttca caacgaaaaa gtattctgaa acttcataat gtttggactt
      601 ctcttgtagg aagtgctatt actattggtt ttggtggatc agttggagct gaagcgccaa
      661 tagtattaac tggatcagcc attggttcaa atttgggaaa atttttcaaa atggaccaaa
      721 aaacacttat gttactcgta ggttgtggag cagcaggtgc tatcgctgga atttttaaag
      781 ctcccattgc agggctggtt ttcacccttg aagttttaat gctggatatg actatgactt
      841 ctattgttcc gctacttatt tcgtcagtta cggcaacatg ttttacctat attttgaccg
      901 gcgatgaatt tatgttccaa tttactcact atgaaccttt taatgtttcg cgaattcctt
      961 acttaatatt attgggtgtt atttgtgggt tggtttcttt atattttaca aggggaacat
     1021 ataaacttga aaattttttt cagaaaattg aaaatcctta taaaaaaatt gctattggcg
     1081 gaagtatcct tagcgtactc atttttttct ttccgccgct ctatggtgaa ggttacgata
     1141 ctatttccac attattaaat ggtgatgttt cttctgtact aagccagagc ttgtttttcg
     1201 gtcacgaaga taattcctgg gctttaattt cttatctcgt ccttattatt atttttaaag
     1261 tttttgcttc tatttcaacg accagtgccg gtggagtagg tggaattttt gcacccagcc
     1321 tgtttgtagg atgtattaca ggatttgtag tggcaaaact tttaagactc atgggagtac
     1381 ctatacccgt tgaaaatttt gctctggcag gaatggcggg gttgatgtca ggcgtcatgc
     1441 atgctcccct gacaggaatt ttcttaatcg cggaacttac aggtggttat tatttgttta
     1501 tgactttaat tattgtctca accatttctt atttaactat cattttattt gagcctcaca
     1561 gcatttatgc catgcgactg gcacaaaaag gtgagttgtt aacccataac aaagataaag
     1621 cagtcttgac gatgcttaaa atgaataatg tcattgaaaa ggatctaatc attcttcatc
     1681 cagaaatgac acttggagaa ttagtgaaag ttattgctca ttccaagcga aatatttttc
     1741 cagtggtaga ttcaaaaact ggcgttttgt tgggggtatt atcactggat gaggtaagaa
     1801 atattatgtt tcgtcgagaa ctatatcatc gatttacggt agggcaatta atggtttcac
     1861 caccagccat tcttaatatt aatatgccta tggaaaaagt aatggaaatt tttgaagata
     1921 caggagcctg gaatttgcct gtggtggatg atcaaaaacg ttacattggt tttgtttcga
     1981 aatcaactat ttttaattct tatcgacatt tacttgtaca tttttcagat gaatgagaat
     2041 agacaatttg ctcttttgcc taaatttaca taaaagccgt atgagataac ataaacaatt
     2101 agaaaaaata aaaactatca attttatgaa cgaacttact tataacgaag caataaatga
     2161 acttgaaaaa attttggagg aactggaaaa caatacagaa attaattttg accagattac
     2221 ccaaaaagtt caacgagcta cagaattgtt ggaattttgt aacaaaaaac ttcatcaaat
     2281 tgatgaagat atggaaaaac tactttccaa gcttgatgaa tcgatcacac aataattaca
     2341 gaagctatta aattctccct aattttcaga aagaaataat tattctagca tccgaaaata
     2401 cgcttaatta tttcctataa ttattcaatt tggtcgtctt tgttgtcgtt acctccttta
     2461 tgttattccc ccaatttttt tataaaaaat cttcttttac ccacaattat aatttcacac
     2521 cgtgcaaatt tatatttcta agcttttgat ttcaaacata gatatcccta ttcataataa
     2581 gatatctatt tcgattattt ttcttatcat actaacaaaa tgaatcattg gctaacttga
     2641 aggtaatcat tttattcaaa tttctttatg aatttactat tttgttatga taaatttaaa
     2701 atttaaagag aaatgattac ctttgctaac aaattaatga caaattacct tttaaaaatt
     2761 atatgagcta ttttattcaa cccaataatt ataagccaat tttagatttg cagcaaactg
     2821 aattgggaat aaaaaaaata aaggattttt ttcaatccaa tctttcttcg gaattacgtt
     2881 tgcgacgtgt tacggctccg cttttcgttt taaaaggaac gggtatcaat gacgatttaa
     2941 ctggtgtaga acgtgccgtt agttttccta tcaaagatat gaatggcagc gaagctgaag
     3001 tagtgcaatc tttagcaaaa tggaaacgtt tggctttagc tgcttatggc attcaacaag
     3061 gctatggaat ttatacagat atgaatgcca ttcgagccga tgaagaatta ggcaacctgc
     3121 attcattata tgtagatcaa tgggattggg aaagagtaat tagcgaagaa gagcgaaatc
     3181 ttaatttttt aaaaaaaatt gtaaaacaaa tttatgctgc ttttctacga acagaatatt
     3241 tagtttggga aagttttcca cagattgaac catatttgcc tcaagaaatt gtatttattc
     3301 atgctgaaga acttcgtcag ctttatcccc atctttcacc aaaagaaaga gaaatagaat
     3361 ttactaaaat tcacaaagct gtctttttga ttggaatagg tgcccctctg ggcgatggca
     3421 aaaaacatga tgggcgtgca ccagattatg atgattggtc cactatagca gaaaatggtt
     3481 tgccaggttt aaatggcgat attctttttt ggaatcctgt tttagaaact gcttttgaaa
     3541 tttcttctat gggcatccgt gttgacaaga cagctctttt acggcagtta gagatggaag
     3601 gggcacaaga tcggttacaa atgtattttc acaaatgttt gctggatggt ttgttacctc
     3661 tttcgatagg tggtggaata ggacaatcac gtttatgtat gttttttctg cgaaaagctc
     3721 atattggaga aattcaatca agtatatggc cgaaggaaat gcaggaaaaa tgtgctaaaa
     3781 tgaatatcat actcatgtaa ttttttcttt tgcatattta atttgtatta tccccagaag
     3841 aaacaacttt ttggggattt tttatgaaaa tttaaagcaa aaaaggaatc aaccctatca
     3901 aattattttc taaaaagtag tttgcttgtt taggcaaaag aaaaaagata aggtaaagta
     3961 aaatttgttt ttaaaagatt tgaaaaccat gaaaaccaat aagaaatttc ttatttgact
     4021 aaaattgaat tcaaaaaata atttagtaac cctaacaaaa tcagaatatt aaaagatatt
     4081 cttgaaactt tttatccttt tacaagtaat tataagctat tttttcgtac tttcgcttat
     4141 cattttaaca aaatcaaata aaatggagat tgactttaaa aaaatgaatg gtttagtgcc
     4201 agctatcatt caggatgaat gtacttcaaa agtattgatg ttgggttata tgaatgaaga
     4261 agcactggct aaaactcttg aaacaggaaa agttactttt ttcagtcgaa gcaaaaatcg
     4321 tctttggaca aaaggagagg aaagtggcaa ttttttgaac ctggtttcta ttactcccga
     4381 ttgtgataat gatacattgt tgataaaagt tcatcctgag ggtcctgtgt gtcatacggg
     4441 caaagatact tgctggggag aatcgaatga aggtgatttt gcttttttaa aatatttgca
     4501 agaattcatt caaaaacgtt ttaaagagat gcccgaaggt tcatatacca cttctttgtt
     4561 caaagaagga ataaatcgca tagctcaaaa agtaggtgaa gaaacaatag aaaccataat
     4621 agaagcaata aatagtaata aagaaggttt tatttacgaa gcttccgact taatttatca
     4681 cttgattgtt ttgttgactg cacaaggatt aactcttgat gatattggaa aagaattaaa
     4741 acgaaggcat aaataagaag ttgataatgt tatggaaaaa aatcttttga ttaaatacgt
     4801 ggatgttgat atttatcaac aagaaatgtt ggttttaaag aacatccaat tagaaattta
     4861 ttcaggtgaa tttatttatt tgttaggaaa agttggcagc ggaaaaactt ctttgttaaa
     4921 atcattttat catgaagtac cactttcgga aggaaatgcg gcagttttaa attatgactt
     4981 acaaaaaatt aagtctaaag aaattcctta tttacgaagg agaattggta ttatttttca
     5041 agattttcag ttacttacag atcggaccgt gtatgctaat cttgaatttg tgttgcgggc
     5101 tactgggtgg aaagagccaa aagccattga tgaacaaatt aatgttgttt tagaatatgt
     5161 aggcatggaa aacaaaggtt ataaaatgcc tcatcaactt tctggcggtg aacagcaacg
     5221 cattgctatt gcacgtgcct tgctaaattc tcctgaaatt attttagccg atgaacctac
     5281 aggaaattta gacaagcaga ctgggaatga cttagttgag ctattatata atattcattt
     5341 ttcaggagct accgtcatta tggccactca caatatgaac tgggtagaaa aatttcctgg
     5401 acgtatcatt aaatttgaaa atgaaatgtt aatcgaagcc tgagataaaa attccaaacg
     5461 ataggtaatt ttagtcaggt aatttatttt tcaaaaatca gaaatacttc caaatatgaa
     5521 taatgactaa aaaatataat ttccttcaaa aaaattgaaa taatttgaaa gattcaaaga
     5581 atgacaaaaa acatattgtg ttgtaagcaa ataaagactc aattattggc aacaaccata
     5641 aatttaaaat tatttacaaa acaattttgc ttgcaaaagt tttaaactcg ttacttgcag
     5701 attaatttgt aatttacaaa atttttttct aaataagata aaaatgatat tgcgtaccga
     5761 acatttagtt aaaaaataca gcaaacgaat tgttgtggat gatgtttcta ttcaagtagg
     5821 acaaggcgaa attgtaggtt tacttggtcc caatggtgct ggcaaaacta ccacttttta
     5881 catggtagta ggattagtaa agccgcttgc aggggaaatt tttttaaatg aacaaaatat
     5941 tactaatttt cctgtatata aacgtgcaca ggcaggtatt ggctatttgg ctcaagaacc
     6001 ttctgttttt agaaaaatga cggtagaaga caatatcaaa tctgtgcttg aaatgacaaa
     6061 attcagtaaa gaatatcaaa aagaaaaatt agagacttta attactgaat ttaatctaag
     6121 tcatgttaga aaaaatattg gagaccgcct ttctggtggt gaacggcgac ggactgaaat
     6181 tgcccgttgc ttggctattg agccacgttt tattctgttg gatgaacctt ttgcaggcgt
     6241 tgatccaatt gctgtgcagg atattcagga tatcgtttgg aaactaaaag acaaaaatat
     6301 tggtatttta attactgacc acaatgttga tgagacatta gccatcaccg accgagcata
     6361 tatgcttttt gatggaaaaa tcttatttca gggtacagcc aaagaattgt ctgaaaatcc
     6421 aattgttcgt gaaaaatatt taggacgcga ttttgagtta aaaaagaaaa ctaaagttta
     6481 aactttttat cactttcct
//
LOCUS       DAOSAS010000105         6194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_8057_length_6194_cov_12.089265, whole genome shotgun sequence.
ACCESSION   DAOSAS010000105 DAOSAS010000000
VERSION     DAOSAS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8057_length_6194_cov_12.089265"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..2089)
                     /gene="rho"
                     /locus_tag="PLR72_05710"
     CDS             complement(230..2089)
                     /gene="rho"
                     /locus_tag="PLR72_05710"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOK36729.1"
                     /translation="MSNYTILQLKAKELPELKEIATELGIKVSNSITKDQLVYNILDQ
                     QAIVNAQQKTTANNSPEKSKKKRTRVTIKNEEQKILSIQTTNSPEQPSTIPSPTPSKI
                     SATNQEIKTEKTEKTSKTPAKRSKKSTQSTQQKTTTTSSSKTLIKKNIAENMEENKDT
                     DLQNEKLKIPLEEIDNFDIPNDKLLPEESTEDQLGTDYATMISTVQIPEHPAQEKKII
                     STHLKDTKTETSVPVKINQRFEKNDKQFDFDGILEGTGTLEIMPDGYGFLRSADYNYL
                     SSPDDIYVSQSQIKLFGLKTGDTVTGTIRPPKEGEKYFPLIKVLKINGRSPEYVRDRV
                     LFEHLTPLFPNEKFKLTTGKNDPLSVRIVDLFCPIGKGQRGLIVAQPKTGKTVLLKEI
                     ANAIAANHPEVYMIVLLIDERPEEVTDMARSVKAEVISSTFDEHAEHHVKVASMVHEK
                     AKRLVECGHDVVILLDSITRLARAYNTVSPASGKILSGGVDANALHKPKRFFGAARNI
                     ENGGSLTIIATALTETGSKMDDVIFEEFKGTGNMELQLDRKLANKRIFPAVDILASST
                     RRDDLLLDQDTLNRMWILRRHLADMNSIEAMEFLKDRMERTENNEEFLLSMNS"
     gene            complement(2346..3680)
                     /gene="tilS"
                     /locus_tag="PLR72_05715"
     CDS             complement(2346..3680)
                     /gene="tilS"
                     /locus_tag="PLR72_05715"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOK36730.1"
                     /translation="MLPQKVELYIEQHHLLEKSSRKIIVGVSGGADSVALLHILLNNG
                     YECICAHCNFKLRGKESERDQKFVHQLSKKLNIPFYTKNFDTIDYAAKNKISVEMAAR
                     ELRYQWFYELLQQLNAQAIAVAHHADDNIETLLMNLIRGTGLKGLTGILCKNDSIVRP
                     LLSVTRTEIENYLIEKNLKYITDSTNLSIDYQRNKIRHKVIPMLEELNPSIKQTLYKS
                     LEHFKGAYDIYQKAIEKIKQKILTQEGDIYKININALNEQVSVLNVLYEVLSPYGFSP
                     DIILQIKENINKSVSGKVFYSDKFRLLIDRKEIIISTFKEEKDQSFLLPAENYEINFP
                     IHLQLKKIIRNANFEVSKEKNLIHVDASLLKFPLVLRHWQEGDSFYPFGMDHPKKLSD
                     FFIDQKLSRLEKEKIWLLLSENKIVWIVGMRLDNRFRITEKTTEILQITLFQ"
     gene            3914..4456
                     /locus_tag="PLR72_05720"
     CDS             3914..4456
                     /locus_tag="PLR72_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOK36731.1"
                     /translation="MTSFLDLVKTRQSVRSYLDKPVEEGKLQRILEAARLAPSACNAQ
                     PWKLIVVDDYEKRMAIAEASSSRILPINHFVRQAPIQIVIVEERENFIAKLGGYARNK
                     HYPPIDIGIVAAHICLAATDEGLGSCIIGWHDEKKIKKILDIPNGKRVMVIILLGYSK
                     QPLRTKHRKSLSEVVSYNKY"
     gene            4492..>4966
                     /locus_tag="PLR72_05725"
     CDS             4492..>4966
                     /locus_tag="PLR72_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HOK36732.1"
                     /translation="MNYDETLQYLYNQLPVFQHIGNAAYKPGFDHVIKILNILHNPQT
                     HFKTIHIAGTNGKGSVSHFLAAILQAAGYKVGLYTSPHLVDFGERIRINGEKIDKQYV
                     VDFVKNYQDFIKEIQPSFFEVATSMAFDYFSFCNIDVGVIEVGLGGRLDSTNVIQP"
     assembly_gap    4967..5066
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5067..5533
                     /locus_tag="PLR72_05730"
     CDS             <5067..5533
                     /locus_tag="PLR72_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HOK36733.1"
                     /translation="HFEIPEQALQEGLEFVTELTGLQGRWQQIQSSPRVILDTGHNLG
                     GIRYIAEQLENQDCKNLRIVFGMVNDKDIDGVLSLMPRKALYYFTQAAIHRALPAEIV
                     LEKANRYGLNGKIYYSVAEAVSNALMEAEPDDLIFIGGSNYIVGEALQFFNK"
     gene            complement(5611..6162)
                     /gene="def"
                     /locus_tag="PLR72_05735"
     CDS             complement(5611..6162)
                     /gene="def"
                     /locus_tag="PLR72_05735"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOK36734.1"
                     /translation="MILPIYVYGNPILRKVAKPISKDYPDLKNLVNNMYETMYHAEGV
                     GLAAPQIGLPIRLLVIDLAPLKKEDTELGNFKITMINPTILEQSSDLVSGEEGCLSIP
                     GIHENVLRPRKIKINYFDIDFNEYTDEFEDYKARVIQHEYDHLEGHLFTDKVNPLRRQ
                     LLKSKLTGIAKGKTPCNYQIKIV"
BASE COUNT         1974 a          925 c          998 g         2197 t
ORIGIN      
        1 aaaaacagtt gattaccttc gtcgttatca agggaaagaa atagagatag acacggttta
       61 tcgttttttg gacaaattaa gcgggagact caaccctcat tttacaggcg caaagttaaa
      121 aaataaaaaa caacattgtt taaaataagc aggataaatt agacgtaaat tcaataaatt
      181 gaaattccct tttgtgcaat aagattttgt agaaaaaata gaaattgggt taactattca
      241 tggaaagcaa aaattcttca ttattttcag ttctttccat tcggtctttt aagaactcca
      301 tggcttcaat agaattcata tcggcaagat gacgacgcaa tatccacatt cggttcaggg
      361 tatcctgatc aagtaataaa tcgtcgcgtc tggtgctgga agccagaata tccacagcag
      421 ggaaaattcg tttgtttgcc aactttctgt cgagttgtaa ttccatattt ccagtgcctt
      481 taaactcttc aaaaataaca tcgtccattt tagagcctgt ttcagttaaa gcagtggcaa
      541 taatggttaa actaccacca ttttcgatat ttctcgcagc accaaaaaaa cgtttaggtt
      601 tatgtaaagc atttgcatca acaccaccag aaagaatttt tcctgatgct ggtgacacgg
      661 tattataagc tcgagctaaa cgagtgatag aatccaataa aataacaaca tcgtgaccac
      721 attcaaccaa tcgtttagct ttttcgtgta ccatggaggc tacttttaca tggtgttcgg
      781 catgttcatc gaaagtagaa gaaataactt cggcttttac gcttcttgcc atgtcggtaa
      841 cttcttctgg tcgttcatca attaacaata caatcatata tacttcagga tgattagctg
      901 caatagcatt tgcaatttcc tttaacagga cagttttacc tgtttttggt tgagcaacaa
      961 tcagtcctct ctgacctttc ccaatgggac agaataaatc tacaattctt acagaaagtg
     1021 gatcattttt tccagtagtt aatttgaact tctcattggg gaaaagaggt gttaaatgtt
     1081 caaaaagaac tctgtctcta acatattcgg gtgaccgtcc attaattttt aataccttaa
     1141 taagtggaaa atatttttca ccttcttttg gaggacgtat ggtacccgtc acggtatctc
     1201 ctgtctttaa accaaaaagt ttaatctgcg attgagaaac ataaatatca tctggagaag
     1261 ataaatagtt ataatctgcc gagcgtaaaa aaccataacc atcgggcata atttcaagag
     1321 taccggtgcc ttctaaaatt ccatcaaaat caaattgctt gtcattcttt tcaaatcgtt
     1381 gatttatttt tacaggaaca gaagtctcag tttttgtatc ttttaaatga gtcgagatta
     1441 ttttcttttc ttgtgcggga tgttctggaa tttgaacggt tgaaatcatt gtggcataat
     1501 ctgtacctaa ttgatcttca gtagattctt ctggtaataa tttatcgtta gggatatcaa
     1561 aattatcgat ttcctcgaga ggtattttca atttttcgtt ttgtaaatcg gtatctttat
     1621 tttcttccat gttttctgca atatttttct taattagagt tttagaagat gaagttgtag
     1681 tggttttttg ttgagttgat tgagttgatt tttttgaacg tttagcagga gttttagaag
     1741 tcttttctgt cttttctgtc ttgatctctt gattagtagc tgaaatttta gaaggggtag
     1801 gagagggaat ggttgatggt tgttctggag aattggtagt ttgaatagat aaaatttttt
     1861 gttcttcatt tttaattgtt acccttgttc gcttcttttt gctcttttct ggactattat
     1921 ttgctgtggt tttttgctga gcatttacaa tagcttgttg atcgagaatg ttatatacta
     1981 actgatcttt ggttatagaa ttggacactt taattcctaa ttctgtagca atttctttta
     2041 actcagggag ttcttttgct tttaattgca aaattgtgta attactcata aattataaat
     2101 caatattatt ataaattaat atttttgaag gaatgattaa tgaaaaagta aattaaaaat
     2161 aaaaaatgta tttttaaagt taaaaagata tggagttaat cggttgttaa aatattaagc
     2221 agagatgctt taataccgga cttaattagt aagttaggaa tccactgcaa aattaaacat
     2281 taatttcaaa tctgcaaaat tactttatgc tcttttcaat aatctcaggt aaaagaatga
     2341 aaaaactatt gaaataaagt aatttggaga atttcagttg ttttttctgt aatccgaaat
     2401 cggttgtcaa gtctcattcc tacaatccaa actattttgt tttcggaaag taacaaccag
     2461 attttttctt tttccaaccg actcaatttt tgatcaataa agaaatcact taactttttt
     2521 ggatgatcca tcccgaaagg ataaaagcta tcgccttctt gccaatgtct caataccaat
     2581 ggaaatttta ataatgaagc atcaacgtgt attaaatttt tttcttttga aacctcaaaa
     2641 ttagcattac gaattatttt ttttaattgc aaatggatgg gaaaattaat ttcgtaattt
     2701 tctgctggca aaagaaaaga ttgatctttt tcctctttga aagttgaaat gattatttct
     2761 ttccgatcta ttaataaacg aaatttgtct gaataaaata cttttcctga aacagattta
     2821 tttatgtttt ctttaatttg aagaataata tcaggtgaaa acccataagg agaaagtact
     2881 tcatataaaa catttaaaac agaaacttgt tcattaagtg cgttaatatt tatcttatat
     2941 atgtcacctt cctgagttaa aattttttgt ttgatttttt cgattgcttt ttgataaata
     3001 tcataagcac ctttaaaatg ttccagactt ttgtataaag tttgtttaat tgatggattt
     3061 aattcttcca acatcggaat cactttgtga cgaattttat ttctttgata atcaatggat
     3121 aaatttgtag aatctgtaat atattttaaa tttttttcga ttaaataatt ttcaatttct
     3181 gttcttgtaa cagaaagtag aggacgaaca atactgtcgt ttttgcatag aattccggtt
     3241 aaacctttta aacccgtccc tcgaattaga ttcattaaca aagtttcaat attgtcgtct
     3301 gcatgatgag ctacagcgat tgcctgagca ttgagttgtt gaagcaactc ataaaaccac
     3361 tgatatcgta attctcgagc tgccatttca acagaaatct tgttttttgc agcataatct
     3421 attgtatcga aattttttgt ataaaaaggt atatttaatt ttttagaaag ttgatgcaca
     3481 aatttttgat ctctttccga ttctttgcca cgcaatttaa aattacaatg agcacaaata
     3541 cattcatagc cattatttaa taaaatatgc aacaaagcca ccgaatcagc tcctccactt
     3601 actccaacga ttatttttct actacttttt tctaataaat gatgttgttc aatatataat
     3661 tctacttttt gcggtaacat attctcattt ttatattata tttgttggaa ataaaccgat
     3721 gacttatttt tttaaatcag aaagttattg attattttgt taattgcaaa gatacattat
     3781 tttttttcta tatttgattg agggtaagtc cttttattcc tttaaaaatg cttgattttg
     3841 ttattaagaa gggaataaat atacctcaaa gagattgata aggattttaa taaaaactat
     3901 taatttcatt attatgactt catttttaga tttagtaaaa acccgtcaaa gtgttcgttc
     3961 ttaccttgat aaaccagtag aagaagggaa acttcaacga atattagaag ccgctcgttt
     4021 ggcaccatcg gcttgtaatg cacagccttg gaaattaata gtagtagatg attatgaaaa
     4081 acgtatggca attgcagaag cttcttcaag cagaatttta ccgattaatc attttgtgag
     4141 acaagcaccg attcaaattg ttattgtaga agaaagagaa aattttattg caaagttagg
     4201 tggttatgcc agaaataagc actatccacc tattgatatt ggcattgttg cagcacatat
     4261 ttgtttggct gctaccgacg aagggctagg ctcgtgtatt attggttggc atgatgagaa
     4321 aaaaataaag aaaattcttg acattccaaa cggtaaacgg gtaatggtta ttattttgct
     4381 gggttatagc aaacagcctc tacgtaccaa acatagaaaa agtttaagtg aagtggtatc
     4441 gtacaataaa tattaatttc aaaaaaatga ctcgattgaa tttgttgaaa aatgaattat
     4501 gatgaaacgt tgcagtattt atacaatcaa ctgcctgtat ttcagcatat cggtaatgct
     4561 gcatataagc ctggttttga tcatgttata aagatattaa atattctcca taatcctcaa
     4621 actcatttca aaacaataca tattgccggc accaacggaa aaggttctgt ctctcatttt
     4681 ttagctgcta ttttacaagc tgcaggctat aaagttggac tttacacttc accgcatctg
     4741 gttgattttg gagaacgaat cagaataaat ggggaaaaaa ttgataaaca atatgttgtt
     4801 gattttgtaa aaaattatca ggattttatc aaagaaatcc aaccttcatt ttttgaagtt
     4861 gctacctcta tggcttttga ttatttttct ttctgtaata ttgatgtcgg cgtaatagaa
     4921 gttggtttag gaggtcgttt agatagtaca aatgttattc agccagnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnntgca tttcgagata cctgaacaag ctttgcaaga
     5101 aggtttggaa tttgtaactg aattaactgg tttacaagga agatggcagc aaattcaatc
     5161 ctcacctcga gtaattctgg atacgggtca taatctgggc ggtattcgtt atatagcaga
     5221 acagcttgaa aatcaagatt gtaaaaatct aagaattgtt tttggaatgg taaacgataa
     5281 agatattgat ggagttttat ccttaatgcc tagaaaagcg ttatattact ttacccaagc
     5341 agctattcac cgagctttgc ctgctgaaat agttttagaa aaggcgaatc gttatggttt
     5401 aaacgggaaa atttattatt cagtagctga agccgtttca aatgctctta tggaagctga
     5461 gccagatgat ttgattttta ttggaggcag taattatatt gttggagaag ccttacaatt
     5521 tttcaataag taaaattaat ccaactccat tgagcacctt ttttaaaaaa tcatttaaaa
     5581 tcaattaaaa accttcttaa ccattcaaga tcaaacaatt tttatttgat aattgcatgg
     5641 agtttttcct ttggcaatac ctgtcaattt ggattttaaa agttgacgac gcaatggatt
     5701 tactttatcg gtaaatagat gtccttccag atgatcatac tcgtgttgaa tgacgcgagc
     5761 tttataatct tcaaattcgt cagtatattc attaaaatca atatcaaagt agttaatttt
     5821 aatttttcgt ggtcttaaaa cattttcatg aattccagga atactcaggc aaccttcttc
     5881 accacttact aaatcagaac tttgttctaa aatggtagga ttaatcattg taattttaaa
     5941 atttcctaat tcagtatctt ctttttttaa aggtgccaga tcaatgacta acaggcgaat
     6001 aggcaaacca atttggggag cagccagtcc aactccctct gcatgataca ttgtttcata
     6061 catattattc actaaattct ttaaatcagg ataatcttta gaaataggtt tagcaacttt
     6121 tcgtaaaatt ggatttccat aaacataaat tggtaaaatc atagttttaa ttcgataaaa
     6181 tataattttt taat
//
LOCUS       DAOSAS010000106         6193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_8060_length_6193_cov_14.295373, whole genome shotgun sequence.
ACCESSION   DAOSAS010000106 DAOSAS010000000
VERSION     DAOSAS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8060_length_6193_cov_14.295373"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..347
                     /locus_tag="PLR72_05740"
     CDS             174..347
                     /locus_tag="PLR72_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK36735.1"
                     /translation="MAYVITEDCIACGSCITECPVDAISEGDIYVIDPEVCTDCGVCA
                     DVCPSEAIIPGGD"
     gene            complement(402..1412)
                     /locus_tag="PLR72_05745"
     CDS             complement(402..1412)
                     /locus_tag="PLR72_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36736.1"
                     /translation="MKFKGIIFTSIVITLLFYSCKKTNRFAINTSKDGIKVVIQRFDK
                     DLILLDTINTEVAVKHLYKKYPDFFPVYISSILEMNPQDTLKICDLIKNFLKDTTFVK
                     VNKKVLEEYADVSDIETTLSTAFTYIHYYFPEIKLPTVYFFVSGFNRAILWNDHLIGI
                     GTDLYLGNDYPLYKEITYEYMIYNMRRECIPADIVSTLLVNSFPMKSSQDRLLENILY
                     RGKIMYLLSIFLPEEKPTDLIGYTSAQWEWCKKYEKEIWASVIEQKALFSSDSKTIGA
                     FINDAPFTAPISQDSPGRLGTWLGWQIIKSYMETNKNVDLRQLMNERNYEKLLENSNY
                     HP"
     gene            complement(1487..3391)
                     /gene="yidC"
                     /locus_tag="PLR72_05750"
     CDS             complement(1487..3391)
                     /gene="yidC"
                     /locus_tag="PLR72_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOK36737.1"
                     /translation="MDKNTFIGFLLIAVILIVFSWLNRPSPEMVEKQKRYNDSIARVQ
                     QIKEESEAVKHQADSLLKSPLLKKDSLTNSILEDAYGDFSVAAVGEEKFITIENEFLK
                     ITFTNKGAQIYSVELKDYRRHDSLPLILFEGKENKMNFTFITNNNRIVNTENLYFEPL
                     PEIKKDKNGNQTIIFRLKTTGEGYLDFAYTIPPKDYMIGFGIKAYQMNNVLPTSINSL
                     EMQWISKIRQQEKGRQFESRYSMIQYKYLSDDTEKLNPNKDESKEIANKIKWIAFKDQ
                     FFSSVLIAEDAFTSTTLKSTIEKTDSPYLKSCSAEMTVPFDISGKEPTVFRMYFGPNK
                     FNILASYDKGVDKNEKLKLQSLVPLGWGIFGWINRFLIIPMFNFFSKFISNYGIIILL
                     MTIVIKIIIFPLTYKSYTSSAKMRVLKPEIDEIYKRIPPEKAAERQKAVMDLYSRVGI
                     SPMSGCIPALLQIPILFAMFSFFPASIELRQQRFLWAEDLSTYDSILNWNAYIPIITP
                     YFGNHISLFCLLMTISSIVATRLNMANSTPSDQPGGNLMKWMMYLMPVMFMFIFNNYS
                     AALSYYYFISTLITIIQTYVIRKMVDEKKLLAELHAKRNTKKPVKKSSFIERLEKMQR
                     EQEKTLKGRK"
     gene            complement(3437..5059)
                     /locus_tag="PLR72_05755"
     CDS             complement(3437..5059)
                     /locus_tag="PLR72_05755"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOK36738.1"
                     /translation="MKDTKYIFVTGGVVSSLGKGIIAASIGKLLQSRGFKVTNQKIDP
                     YINIDPGTLNPYEHGECYVTVDGHEADLDLGHYERFLNIETHKENNFTTGRIYQTVIE
                     KERRGDYLGKTVQVIPHITDEIKRNIKLLGSRNGYDFVITEIGGTVGDIESLPYIESV
                     RQLRWELGKNCLVVHLTYVPYIAAAQELKTKPTQHSVKTLQEQGVQPDIIVLRTEHNI
                     PADLRKKVALFCNVEPSAVMQSIDVSTIYRVPLNMHEEKLDEILLSKMGFDLTKTPSA
                     DLDKWRHFVEKLENAKDEIHIALVGKYVQLPDAYKSITESLLQACAYNDKKLQLDLIL
                     SDKLNEDNVAKKLANTDGVIIAPGFGQRGIEGKFAAIKYTREHNIPTLGICLGMQCMA
                     IEFARNVLGYADANSTEFDPTTSHNVVDIMEEQKNILNLGGSMRVGAYPCSLKKGTKV
                     YEIYQTDNIYERHRHRFEFNNDYKEEFEKAGMICSGINPASNLIETIELQSHNWYIGV
                     QFHPEYHSTVVNPHPLFISFIKAAIEKQNKKI"
     gene            5431..5922
                     /locus_tag="PLR72_05760"
     CDS             5431..5922
                     /locus_tag="PLR72_05760"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOK36739.1"
                     /translation="MKIKIFLNCLIILSLMTITFSGCKSKQKVTEIPGANVKATTTSP
                     STTPSETTAPVVVTTPPSETETNIPEVTRKESFNLAEDETNRDALSYKYHVVVGSFKN
                     LSNAKGLQSTLKQEGNNSLIVINEQGMYRVIIDSFNDYYQAHARINQIKNRFPDAWVL
                     IQK"
BASE COUNT         2095 a         1017 c          935 g         2146 t
ORIGIN      
        1 tttaggttta atacgaatca agtgtagaaa tctctcaatg tttaaataaa ttaaccttga
       61 aagagttttc ggagtggact cagattttaa aataagaaaa aattaaaatt tttttttgat
      121 atagaaataa tgtgtaattt tgcggtaaat ttattaaaat aaaaaataga aaaatggctt
      181 acgtaattac agaagattgt attgcttgtg gttcgtgtat tactgaatgt ccagttgatg
      241 caatatcaga aggtgatatt tatgtaatag accctgaagt atgtacggat tgtggagtct
      301 gtgctgatgt ttgtccatca gaagcaatta tacctggtgg ggattaattt tttttaaaat
      361 aatgcggctt attcttttct gtaaaagagt aagccgtttt tttatggatg ataattggaa
      421 ttttcaagta atttttcata atttctttca ttcataagct gtctcaaatc tacattttta
      481 tttgtttcca tataactttt tataatctgc cagccaagcc acgtgccaag tcgtccagga
      541 gaatcctgcg aaatgggtgc agtaaatgga gcatcgttta taaatgctcc aatagtttta
      601 gaatcggacg agaataatgc tttttgttca ataacacttg cccaaatttc tttttcgtat
      661 tttttacacc attcccattg agcagaagta taacctatca aatctgtcgg tttttcttca
      721 ggtaaaaaaa tagacaataa atacatgatt tttccacgat aaagtatgtt ttcaagcaaa
      781 cgatcctgag aacttttcat tggaaaggaa ttgaccagca aggtagaaac aatatcagca
      841 ggaatacatt cacgtcgcat attataaatc atgtattcat aagtaatttc tttatagagc
      901 ggataatcgt tgcccaaata taaatctgtt ccaataccaa ttaaatggtc gttccataaa
      961 atagcccgat taaaccctga aacaaaaaaa taaacagttg ggagtttgat ttcagggaaa
     1021 taataatgaa tataagtaaa agcagtagaa agagttgttt caatatcaga aacatcggca
     1081 tattcttcaa gcactttttt atttaccttt acaaaagtgg tatcttttaa gaagtttttt
     1141 attaaatcgc atatttttaa agtgtcttgt gggttcattt ccagaataga agaaatataa
     1201 actgggaaaa aatcaggata tttcttgtat aagtgtttaa cagcgacttc cgtattgatt
     1261 gtatcaagta aaatcaaatc tttatcaaac cgttgaataa ccacttttat tccgtctttt
     1321 gaggtattta tggcaaaacg attcgttttt ttacacgaat aaaaaagcaa agtaataacg
     1381 atgcttgtaa aaataattcc tttgaatttc ataggtaaaa aattaaaaaa ataaacaaca
     1441 aaaataatat tttctacttt tagtttaatt agttggaata caaaacttat tttctgccct
     1501 tcaaagtttt ttcttgctcg cgttgcattt tttctaaacg ttcaataaaa ctactttttt
     1561 tgacaggctt tttagtattg cgtttagcat gcaattctgc caataatttt ttttcatcaa
     1621 ccattttgcg gataacatag gtttgaatga tagtgattaa agtggaaata aaatagtaat
     1681 aactcaatgc tgccgaataa ttgttgaata taaacataaa catcacgggc atcaaataca
     1741 tcatccactt catcagattt ccaccaggct gatcggaagg tgtagaattt gccatattta
     1801 atcgagttgc tacaatactg ctgatggtca tcaacaaaca aaacaagcta atatggtttc
     1861 caaaataagg agtaataata ggaatataag cattccagtt taaaatagaa tcataagtag
     1921 aaagatcttc tgcccaaaga aatctttgct gtctgagctc gatggaagca gggaaaaagc
     1981 taaacatcgc aaacaaaata ggaatttgca gcaaagctgg aatgcatcca ctcatcggac
     2041 tgatacctac tcttgaatat agatccatca ctgctttttg acgttctgct gctttttctg
     2101 gaggaatacg tttgtaaatt tcatctattt ctggtttcaa aacacgcatt tttgcactcg
     2161 aagtatagga tttataggta agggggaaaa ttataatctt aataacaata gtcattaaaa
     2221 gaataataat tccataatta ctaataaatt tactaaaaaa gttgaacatt ggaataataa
     2281 gaaaacgatt aatccatcca aaaattcccc aaccaagcgg cactaaactt tgaagcttta
     2341 atttttcgtt tttatctacg cccttgtcat aacttgcaag aatattaaat ttattaggac
     2401 caaagtacat ccgaaataca gtgggttctt taccagaaat atcaaaaggc accgtcattt
     2461 cagcactaca tgattttaaa taaggtgagt ccgttttttc tatcgtactt tttaatgtgg
     2521 ttgaggtaaa agcatcttcc gcaatcaaaa cactgctgaa aaattgatct ttaaaagcaa
     2581 tccattttat tttattagct atttctttgc tttcgtcttt attaggatta agtttttccg
     2641 tgtcgtccga taaatattta tattgaatca ttgagtaacg tgattcaaat tgccgtcctt
     2701 tttcctgttg acgaattttt gaaatccatt gcatttcaag cgaattaatg cttgttggca
     2761 aaacattatt catttgataa gctttgatac caaagcctat catataatcc tttgggggaa
     2821 tagtataggc aaaatccaga taaccttctc cggtggtttt cagacgaaaa ataatagttt
     2881 gatttccatt tttgtccttt ttgatttcag gaagaggttc gaaatacaaa ttttctgtat
     2941 tgacaatacg attgttattg gtaataaaag taaaattcat tttgttttct tttccttcaa
     3001 ataatattaa aggcaaagaa tcgtggcggc ggtaatcttt cagttccacc gaataaattt
     3061 gagctccttt attagtaaaa gtaatcttta gaaattcatt ttcaatagtg ataaattttt
     3121 cttctcctac agcagctaca ctaaaatctc cataagcatc ttcgagaata gaattggtaa
     3181 gtgaatcttt tttgagtaaa ggtgatttca gcaaagaatc agcctgatgt tttactgctt
     3241 cactttcttc ttttatctgc tgaactctgg caatagaatc attatatcgt ttttgttttt
     3301 caaccatttc aggcgatggc ctgtttaacc acgaaaacac aatgagaata actgcaatca
     3361 acaaaaaacc tataaacgta tttttatcca tgttttataa atttcaacct atccttttcc
     3421 ttagtgttat aaaaatttaa atttttttat tttgtttttc tatagccgct ttaataaaac
     3481 taataaagag gggatgagga ttcactactg tactatgata ttctggatga aattgaacgc
     3541 caatatacca gttatgcgat tgtaattcaa ttgtttctat gaggttagaa gctggattga
     3601 taccactgca aatcattcca gctttttcga actcttcttt atagtcatta ttaaattcaa
     3661 aacgatgtcg atgacgctca taaatattat ctgtttgata aatttcataa actttcgttc
     3721 ctttttttaa agagcatgga taagctccca ctcgcatact tccaccaaga tttaatatgt
     3781 ttttttgctc ctccataatg tccactacat tatgagatgt agtggggtca aactctgtac
     3841 tatttgcatc agcgtaaccc aatacatttc gagcaaattc aatagccata cattgcattc
     3901 ccaaacaaat tcccaatgta ggaatattgt gttcacgagt atattttata gctgcaaatt
     3961 ttccttctat tcctcgttgc ccaaaacctg gcgctataat tactccatca gtattagcta
     4021 atttttttgc tacattatct tcatttaatt tatctgataa aattagatcc agttgtaatt
     4081 ttttatcgtt ataagcacat gcctggagaa gagattcggt aatagatttg taagcatctg
     4141 gaagttgaac atattttcct accagcgcaa tatgaatttc atcttttgca ttttcaagtt
     4201 tttctacaaa atgtctccat ttatctaaat cagcagaagg tgttttagtt aaatcgaatc
     4261 ccattttact cagaagaatt tcatccagct tttcttcgtg catattcaat ggtactctgt
     4321 aaatagttga aacatcaata gattgcataa ccgccgatgg ctccacattg caaaataagg
     4381 ctactttttt ccgtaaatca gcaggaatat tatgttctgt tcgtaaaacg ataatatcag
     4441 gttgtactcc ctgttcctgt aaagttttta cagaatgttg agtgggtttg gtttttaatt
     4501 cctgagcagc agcaatataa ggcacataag ttaaatgaac gacaaggcaa tttttaccca
     4561 gttcccaacg taattgacgt acactttcaa tgtagggcaa ggattcaata tcgcctactg
     4621 tcccacctat ttcggtaatg acaaaatcgt acccatttct gctgcctaat aatttaatat
     4681 tgcgtttaat ttcgtcagtg atgtgaggta ttacctgtac agtttttccc aaataatctc
     4741 ctctccgttc tttttcaata actgtctgat aaattcgtcc tgtagtaaaa ttattttcct
     4801 tatgcgtttc tatatttaaa aatcgctcgt aatgacctaa atccagatcg gcttcgtgtc
     4861 catctactgt gacgtaacat tcaccatgtt cataaggatt taaagttcct ggatcgatat
     4921 tgatataagg atcaattttt tgattagtta ctttaaaacc tcttgattgt aataatttac
     4981 ctattgaggc agcaataatg cctttaccta atgaagaaac aacgccaccc gttacgaaaa
     5041 tatacttggt atctttcact ttacctataa taacttaact taattaaagc tgcaaatttc
     5101 ggaaaaaaaa atgaatattg aaaggataca aagtattttc cttttttatt aaaaaaatcc
     5161 ttgattcttc tgactattaa aaaaaaacta tgtttttttg aaaataacgt ataattctca
     5221 caaattaatt ttaaggattt tataatttgt aagttattga atatcaatac tgaattttaa
     5281 aaattagcga agcaatcttt taaaaataaa tttatgaata tcgaactcaa aaaattggat
     5341 aaaataaaat cataaaattt tgtatatttc taattttgtt ttcatctttg caatgaaaaa
     5401 gcatatttta taatcctaaa aaagaccaat atgaaaatca aaattttttt gaactgtcta
     5461 ataatattgt cgctcatgac aataactttc tcaggttgca aatcaaaaca aaaggtgacg
     5521 gaaatccctg gggcaaatgt aaaagctaca actacctctc cttctactac tccttcagaa
     5581 actactgctc ctgttgttgt aactactccg ccttcagaga ctgaaactaa tataccagaa
     5641 gttacacgta aagaaagctt taacttggct gaagatgaaa caaatcgcga tgctttaagc
     5701 tacaaatatc atgtagtagt tggaagtttt aaaaatttgt caaatgcgaa aggcttacaa
     5761 agcacattaa aacaagaagg taataattct ctaatcgtaa tcaatgaaca aggaatgtac
     5821 agggtaatta ttgattcctt taatgattat tatcaagctc atgcacgtat caatcaaata
     5881 aaaaatagat tccctgatgc gtgggttttg atacaaaaat aaaattattt ttcaaaatcg
     5941 atatttaaaa aaccgttcac taatagaaaa tagtaacggt ttttttgttt tcatttgaat
     6001 aaagaaattc cttaaaattg actatcctcg ccgcaagcag cgaggtatta cgccaatttt
     6061 atggcttcgc caccggaatt tctaaaaaaa taaaaacaaa agctaccccg acgcaaacat
     6121 cggggaattc ttacgattaa aaaagaaatc aaagttgaaa agcttttcgg atttcttcta
     6181 ttttgtccaa ttt
//
LOCUS       DAOSAS010000107         6183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_8077_length_6183_cov_13.192232, whole genome shotgun sequence.
ACCESSION   DAOSAS010000107 DAOSAS010000000
VERSION     DAOSAS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8077_length_6183_cov_13.192232"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLR72_05765"
     CDS             <1..657
                     /locus_tag="PLR72_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK36740.1"
                     /translation="WKVLMQPGFRKAVLIGCAIAILGQFMGVNAVLYYGPSIFESSGL
                     SRGDSLFYQTLIGTVNMLTTILALLIIDKIGRKKLVYVGVSGMIISLILIGLYFSFAN
                     VWGLTSIFLLICFLAYIFFCAGSISAVIFVFLSEMYPTRIRGLAMSIATLSLWIGTYL
                     IGQLTPWMLETLTPSGTFFLFAAMCIPYMLIVWKLMPETAGKSLEDIERFWLKNTKTK
                     "
     gene            719..1834
                     /locus_tag="PLR72_05770"
     CDS             719..1834
                     /locus_tag="PLR72_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HOK36741.1"
                     /translation="MKTKKIDRRNFIKNIAIGSVGLLTAQTLNAHVKSEITKKPLFPQ
                     KKSPPLKKLSNEMFKTPGIFKGMPIHATFLDEISWDIPHQNWGVKEWDKDFQAMKAIG
                     IHTVVLIRSGLGRWIASPFKSILDSEDVFYPPVDLVEMFLTLADKYNMAFYFGMYDSG
                     KYWIEGAFQKEIDLNLKLIDEVWEKYRHHTSFKGWYLSQEISRRTKNMSKVYAEIGKH
                     AKAISNNLPTLLSPYIHGIKTDQVMSGDKPITIQQHENEWNEILSNIEGAVDILAFQD
                     GQVDYHELPDYLEVNKKLADAHKMRCWTNIESFDRDMPIRFLPIKWEKLLLKLEAARK
                     AGMEDAITFEFSHFMSPNSTYLQAKYLYDRYKEHFNF"
     gene            complement(1861..3120)
                     /locus_tag="PLR72_05775"
     CDS             complement(1861..3120)
                     /locus_tag="PLR72_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOK36742.1"
                     /translation="MSDARLNIESLVDLLVEEVQTSRREAEEFFKAFFAALESALLDG
                     ETVTIKNFGIFQVVRHGPHKILNFQNRREFVIPRRCKVIFTPDEILRDLVNKPFSYLE
                     PVELDVEDKDENFVSEEPPLEPLSIFGEQATEIKSLLEEIKNLSPSSLQNETVENVPM
                     KEKKESSEQEKEEENIPEEIIEEKNEEILVSYNNEEQEEIVSPPSIIKSKNKKDNFDE
                     SYKNENLEPLGIPSRPFKGDNLSDIPLPRKRRHFWLPLVLFIIIAGGVSLYYFYSPVN
                     EYVNTLLKIKTDKNLVLSGNKNFITEQPFVTKDSLGEVSKQSANEVDSLQILFDTPRY
                     YSEFITTEKIMEGSRLAGLSRRYYGVPVFWVYIYEANRDRITHPDNIPVGTPIRVPKL
                     DSRLIDISNPRCLEKAKELEKLYLGKK"
     gene            complement(3113..3403)
                     /locus_tag="PLR72_05780"
     CDS             complement(3113..3403)
                     /locus_tag="PLR72_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOK36743.1"
                     /translation="MTQKELIDKIAKNIQLPREEVEKLLWTTSFILIRELSEGKNIEI
                     PDFGNFEIKKREEKLLVHPVTKKRALFPPKLEVQFKQSYALAKKLNKFFKNE"
     gene            complement(3454..4761)
                     /gene="rimO"
                     /locus_tag="PLR72_05785"
     CDS             complement(3454..4761)
                     /gene="rimO"
                     /locus_tag="PLR72_05785"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOK36744.1"
                     /translation="MKKKDTIDIITLGCSKNLVDSEFLMRQFEALGYRVRHDAEVPDG
                     NIVIVNTCGFIADAKEESIYTILQFAKQRKENKIDKLFVMGCLSQRYLKELQEEIPEV
                     DKFYGKFDYVGILKELGENYQSHLRLERTLTTPAHYAYVKIAEGCNRTCSYCAIPLMT
                     GKYQSRPMEDIEKEVKILVSKGVKELQLIAQDLSDYGEDLYKTSKLAELIERLSDIQG
                     VEWIRLHYAYPVRFPFDVLKVMRERDNVCKYLDISFQHISDKMLKLMRRNITKKQTYE
                     LIDRIREEVPGIHLRTTLMVGHPGETEEDIQELKEFIRKEKFERLGAFTYSHEEGTFA
                     YQNYEDEISEEEKLQRLDEIMELQQQISFELNQFKIGKVFKVMIDRVEGEYYVGRTEF
                     DSPEVDPEVLIPISNKNIKIGEFSNVKIIDALDFDLYGTSIVD"
     gene            complement(4818..5768)
                     /gene="ftsY"
                     /locus_tag="PLR72_05790"
     CDS             complement(4818..5768)
                     /gene="ftsY"
                     /locus_tag="PLR72_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOK36745.1"
                     /translation="MSILNFFNKDKKETLEKGLEKTRESVFSKISRAIAGKSKVDDEV
                     LDELEEVLITSDVGVETTLRIIDRIEKRVARDKYVNTNELNTILKEEIAALLAENNIK
                     SVPDFSADLPVKPYVIMVVGVNGVGKTTTIAKLAYQYIKEGKKVFLGAADTFRAAAIE
                     QLSIWSDRVGAHVVKQKMGADPASVTYDTIASAKANDADVVIIDTAGRLHNKINLMNE
                     LTKIRNVMQKVMPEAPHEVLLVLDGSTGQNAFEQAKQFTKATDVTALAITKLDGTAKG
                     GVVLGISDQFKIPVKYIGLGEGLEDLQVFDKMAFVESLFE"
BASE COUNT         2000 a         1107 c          880 g         2196 t
ORIGIN      
        1 tggaaagtac tcatgcagcc aggttttaga aaagctgtgc ttatcggttg tgctattgct
       61 atattgggtc aatttatggg cgtaaatgca gttttatact atggaccttc tattttcgaa
      121 tcaagtggct tatcacgagg cgattcactc ttttatcaga cattaatagg aacggttaac
      181 atgctcacta ctatattggc attacttatt attgataaaa ttggacgtaa aaaactggtg
      241 tatgttggtg taagtggtat gattatttct cttatactaa ttggacttta tttttccttt
      301 gctaatgttt ggggattaac aagtattttt ttgttaatat gcttccttgc ttatatattt
      361 ttttgtgctg gatctattag tgcggttatt tttgtttttc tctctgaaat gtatcccact
      421 cgcattaggg gattggctat gtctattgca actctttcgt tatggattgg cacctatctt
      481 atcggacaac ttactccatg gatgttagaa accttgaccc catcaggaac tttcttcctt
      541 ttcgcagcaa tgtgcatacc atacatgctc atcgtttgga aactgatgcc cgaaacagca
      601 ggaaaatcat tggaagacat tgaacgtttt tggttaaaaa atacaaaaac gaaataaaaa
      661 atttatttca acaagcattt acaaaaataa cctttagtat aaaaattgaa ataaaggtat
      721 gaaaactaaa aaaattgata gaagaaattt tataaaaaat atagctattg gttctgtagg
      781 attattaaca gctcaaactc tcaatgctca cgttaagtca gaaattacaa aaaaaccatt
      841 attcccacag aaaaaatctc cacccttaaa aaaactatcc aatgaaatgt ttaaaacccc
      901 aggaattttt aaaggaatgc caatccacgc tactttcctt gatgaaataa gctgggatat
      961 acctcatcaa aattggggag taaaagaatg ggacaaagat tttcaagcaa tgaaagcaat
     1021 tggaatccac acagtagtat tgattcgttc aggcctgggt cgttggatcg cttcaccctt
     1081 taaatctatt ctggattcag aagatgtttt ttatcctcct gtagatttag ttgaaatgtt
     1141 tctgacctta gctgataaat ataatatggc tttttatttt ggcatgtatg attctggtaa
     1201 atattggatc gaaggtgctt ttcaaaaaga aatagattta aatttaaagc tgatagatga
     1261 agtatgggaa aaatatcgtc atcatacttc ctttaaaggt tggtatcttt ctcaagaaat
     1321 tagtaggcga actaaaaaca tgtctaaagt atatgctgaa attgggaaac atgcaaaagc
     1381 aatttcaaac aatcttccca ccttgttatc tccttatatt catggaataa aaactgacca
     1441 ggttatgagt ggagataaac ccataacaat acaacaacat gaaaatgaat ggaacgaaat
     1501 attaagtaat atcgaaggtg cagtagacat attagctttt caagatgggc aagtagatta
     1561 tcatgaactt ccagattatc ttgaagtgaa taaaaaatta gctgatgcac ataaaatgcg
     1621 ctgctggaca aacattgaat cttttgatcg tgatatgcca atacgttttc tccctataaa
     1681 atgggaaaaa ttattgttaa aactggaagc agcacgtaaa gccggaatgg aagatgctat
     1741 tacctttgaa ttttctcatt ttatgagtcc taattcaact tatttgcaag ctaaatatct
     1801 atacgatagg tataaagaac actttaattt ttaaatttcc gattaataag ctattcactt
     1861 ctatttttta cctaaataca gtttttccag ttctttggct ttttccaaac aacggggatt
     1921 tgaaatatca attaaacggc tatccaattt gggtacacga atgggtgtgc caacaggaat
     1981 gttatctgga tgagtaattc gatcacgatt tgcttcataa atataaaccc aaaaaacagg
     2041 cacgccatag taacgtcttg aaagtccagc taatctgctg ccttccataa ttttttcagt
     2101 tgtaataaat tcactataat atcggggtgt atcaaataaa atttgcaaac tatctacctc
     2161 attcgctgat tgtttactca cttctccaag agaatctttt gtaacaaatg gctgctctgt
     2221 tataaaattt ttatttccac ttaaaaccaa attcttatca gtcttaattt ttaataaagt
     2281 attaacatat tcatttacag gagaataaaa ataatataaa cttacacctc cagcaataat
     2341 aatgaacaga accaatggta gccagaaatg tcttcttttt ctaggcaaag gaatatctga
     2401 tagattatct cctttaaaag gtcgcgaagg aattcctaat ggttctaaat tttcattttt
     2461 atatgactca tcaaaattat ctttcttgtt tttgcttttt ataattgaag gtggagaaac
     2521 aatctcttct tgttcttcgt tattataaga cactaaaatt tcttcatttt tttcttcgat
     2581 tatttcttca ggaatatttt cctcttcttt ttcttgttct gaactttcct ttttttcttt
     2641 catgggaacg ttttcgacag tttcattttg caaagaagaa ggagaaagat tttttatttc
     2701 ttcgagaaga ctcttgattt cagtggcttg ttcaccgaaa atactaagag gttccaaagg
     2761 aggctcttcg ctgacaaaat tttcgtcttt atcttcaaca tcaagttcta cgggttcaag
     2821 gtaagaaaaa ggcttattta caagatctcg taaaatttca tcaggtgtaa aaattacttt
     2881 acaacgacga ggtataacaa attctcttct attttgaaaa tttaaaattt tgtgaggacc
     2941 atgtcgtact acctgaaaaa tgccaaaatt ttttattgtc accgtttctc catccaacaa
     3001 agcactttct aacgccgcaa aaaaagcttt aaaaaattct tctgcttctc tcctactcgt
     3061 ctgaacttct tcaacaagta agtccaccaa cgattcgata tttaatctag catcactcat
     3121 ttttaaaaaa tttgtttagc ttttttgcta atgcataact ttgcttaaac tgaacttcta
     3181 attttggcgg aaagagtgca cgtttttttg tgacaggatg aaccaacaat ttttcttctc
     3241 ttttttttat ttcaaaattt ccaaaatcag gtatttctat atttttacct tcgctgagtt
     3301 ctcgtattaa aatgaaggaa gtagtccaca ataatttttc aacttcttca cgaggaagtt
     3361 gaatattttt agcaatttta tctatgagtt ctttttgcgt cataatagtt tattaaaaaa
     3421 tgaataacct cgaggaattc ttttgattaa aaattaatcg acaatagaag ttccgtataa
     3481 atcaaaatca agggcatcaa taatttttac gtttgagaat tctccaattt taatattttt
     3541 gtttgaaata ggaattaaaa cttctggatc cacttctggt gaatcaaatt cggtgcgccc
     3601 tacataatat tccccttcta ctctatcaat cattacttta aatactttcc caattttaaa
     3661 ttgattcaat tcaaaagaaa tctgttgctg taattccata atttcatcta aacgttgcaa
     3721 tttttcttct tcagaaattt catcttcata attttgataa gcaaaagttc cttcctcgtg
     3781 cgaataagta aaagctccca aacgttcaaa tttttctttt cttataaatt cctttaactc
     3841 ttgaatatct tcttccgttt cacctggatg tcctaccatt aaggttgttc gcaaatgaat
     3901 acctggcact tcttctctaa ttcggtcaat taattcataa gtttgctttt ttgtgatatt
     3961 tcttctcatc aacttcaaca ttttatcgct aatatgctgg aaagaaatat ctaaatattt
     4021 acatacatta tctctttctc tcattacttt cagcacatca aatgggaagc gtacaggata
     4081 agcataatgt aaacgaatcc attccactcc ttgaatatct gaaagacgct cgattaattc
     4141 tgctaatttg gaggttttgt agagatcttc cccataatca gataaatctt gagcaattaa
     4201 ttgcaattcc tttactcctt ttgaaactaa tattttaact tccttttcta tatcttccat
     4261 cggacgtgat tgatatttgc ctgtcatcaa aggaatggca caatatgaac acgttcggtt
     4321 gcaaccttca gcaatcttaa cataagcata atgagcaggt gtggttaaag ttctttccag
     4381 acgcaaatgc gattgataat tttcgccaag ttcttttaaa attcccacat aatcaaactt
     4441 tccataaaat ttatccactt ctggaatttc ttcctgcaat tcttttaaat aacgttgcga
     4501 tagacatccc atcacaaaca atttgtcaat tttgttttct tttcgctgtt ttgcaaactg
     4561 caaaatagta taaatacttt cttccttcgc gtcggcaata aatccacaag tatttacaat
     4621 cacaatattg ccatcgggaa cttccgcatc atgacgaact cggtaaccca aagcttcaaa
     4681 ttgacgcatt aaaaactcag aatctaccaa atttttagaa caacccaatg taataatatc
     4741 aatcgtgtct ttctttttca tgatagtcac ttactggaga tcaaaaatct ttatttaatt
     4801 tgttgataaa taaagattta ttcgaataat gattccacaa atgccatttt atcaaatact
     4861 tgcaaatcct ctaaaccttc acccaatcca atatatttta cagggatttt aaattgatcg
     4921 gaaatgccta atacaactcc acctttcgcc gtaccatcca gtttggttat tgccaaagca
     4981 gtgacatctg ttgcttttgt aaattgcttt gcctgttcaa aagcattttg tccagttgaa
     5041 ccatccagca ccaataacac ttcatgaggt gcctctggca taactttttg cataacattt
     5101 cggatttttg ttaattcatt cattaaatta attttgttat gcaaccttcc agcagtatcg
     5161 ataattacta catctgcatc attggctttg gcagaagcaa tagtatcata agtaaccgat
     5221 gcaggatctg ctcccatttt ttgcttcact acgtgagcac ctactcggtc actccaaata
     5281 gaaagttgtt cgatagccgc tgctctaaaa gtatcagcag ctccaagaaa aactttttta
     5341 ccctctttta tatattgata agccaactta gcaattgttg ttgttttacc tactccattc
     5401 acgcctacca ccataataac ataaggttta acgggcaaat cggcagaaaa gtcaggcaca
     5461 gatttaatat tattttctgc aagtaaagca gcaatttctt cctttaaaat agtgtttaac
     5521 tcattggtgt ttacatattt atctcttgcg actctttttt cgatcctatc aataattcgc
     5581 aaagtcgttt ctacgcccac atcagaagtg attagtactt cctctaattc atccagcact
     5641 tcatcatcta cttttgattt gcctgcaata gctctcgaaa tttttgaaaa aacactttct
     5701 cttgtttttt ctaacccctt ttcgagcgtt tctttcttat ctttattgaa aaaatttaaa
     5761 atactcatat tttttatatt caaccaatta ttatacaaaa ttataaaata tttccgagtt
     5821 acttcgacat tttaaaaaga gaatgagtaa atataacata taaacataaa ttatttttcg
     5881 tgaaaaaatt tcttaggtaa aattacagga gaaaaatcga ttccaaatag gctgatgatt
     5941 gctagtctta acacaataac ataatgaaca atttataaga tttaagctta ttctcttctt
     6001 tcctaacttt aaaaatacca tttttagtgt tatgttttaa aaaataaaaa atatttacat
     6061 ataattttga tattcagaaa tatgtatagt taaatataag ctcaaaaaag tttggcatat
     6121 gttttgaatt tattattatc ccatgcataa gtaccttaaa acaaaaaact tgatttattg
     6181 atc
//
LOCUS       DAOSAS010000108         6132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_8161_length_6132_cov_11.570512, whole genome shotgun sequence.
ACCESSION   DAOSAS010000108 DAOSAS010000000
VERSION     DAOSAS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8161_length_6132_cov_11.570512"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLR72_05795"
     CDS             <1..656
                     /locus_tag="PLR72_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK36746.1"
                     /translation="SLNVSSASVSRNTSKKLFDTSSYLFNYSFSEPQAYATYLATTPV
                     FVATDDKVVGLHVYGDLSGNSIELRFTAGSEVKYVKLCDLNFFGWEFVETKLDNLQEG
                     LPYEFTGFRVVRNEGILSGNGELYFDNMLLYDAPILFVPEILKGKIHIYPNPVSDIIY
                     IQIENEEIPTLQLYSLNGKLLKETKALEMNIQGIVPGTYILIININRQKASMPVIIK"
     gene            complement(848..1786)
                     /gene="trxB"
                     /locus_tag="PLR72_05800"
     CDS             complement(848..1786)
                     /gene="trxB"
                     /locus_tag="PLR72_05800"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOK36747.1"
                     /translation="MSEKVRCLIIGSGPAGCTAAIYAARANLSPVLYQGLEPGGQLTL
                     TNEIENFPGFPEGISGAELMDKMQKQAEKFGAAVRFGMVTAVDFSLRPYKVIVDNEKT
                     IKAETIIIATGAKAKYLGLPDEQKFAGAGVSVCATCDGFFYRNKKVAVVGGGDSACEE
                     ALYLANIASKVYMIVRKPYLRASKILQERVKNHPKIEILFEHETVGLTGEKVVQGANL
                     VKRRGQPDEEQVHIEIDGFFLAIGHQPNSKIFKPWLETDETGYLKTIAGTPCTKLPGI
                     FAAGDVADPHYRQAVTAAGSGCQAAIEAERFLSNQV"
     gene            complement(1848..2483)
                     /locus_tag="PLR72_05805"
     CDS             complement(1848..2483)
                     /locus_tag="PLR72_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-like putative outer membrane lipoprotein
                     chaperone"
                     /protein_id="HOK36748.1"
                     /translation="MKQLFFSITALIISINSLFAQKSADAEKIINDLFYLFTNNAISS
                     NFKLTISEKNNINSQTYNGSFFLKGNKFMIDMNEMKVWYDGKTQWAYSQQNNEVTITE
                     PTAEELINTNPITFLSAYKNKFSAQFCKKSYSELYCIEMFPKTKNESILKVELKVNRT
                     TGDPVSLEINEKNGSTSLLTLINFKKNIKINDDFFVFHPSKYNDVYINDLR"
     gene            complement(2538..4955)
                     /locus_tag="PLR72_05810"
     CDS             complement(2538..4955)
                     /locus_tag="PLR72_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOK36749.1"
                     /translation="MAPKKPIKNSSKSTSKTHSSSPKKENKVSFWKKINFFINDERTH
                     FILGLLFLLIMLYFLLAFVSYFFTGYADQSKIDLSWSELHQMRNSIQNWTSVLGAITA
                     ETLINRWFGVSSFFILFFGIVVALRLMKAVKLSLWKTFFHCAFWLIWLSVTLGFVFSP
                     FYNNVLFFSLGGQHGDYASQWLTSYIGVPGTLMVIIGTLLIYCIISFNSALPFLKQIF
                     SKKQKKQLSPDQNINVAIGTEIVPEEWLSKGVDESNEYFSGQEKSDVPFEIYPVETTD
                     ENKPLLEEEDRVREEENADPNYNFEKLGKYDPTLELAHYYFPTLDLLKVYGTDNNAPI
                     DMDEQIANKEKIISTLQNFDIEIESIKATVGPTITLYEIVPKAGVRISKIRNLESDIM
                     LSLAAIGIRIIAPIPGKGTIGIEVPNNDPQIVSMHSVIASKKFQESKYELPIAFGRTI
                     TNEIFMFDLVKMPHLLVAGATGQGKSVGLNAIITSLLYKKHPAELKLVLIDPKKVEFN
                     IYSGIAKHFLAKLPDDSDAIVTDTSKVVETLQSLCTEMDNRYDLLKKAHVRNIKEYNE
                     KFIMRNLNPEKGHKYLPYIVVIIDEFGDLIMTAGKEVEMPIARIAQLARAVGIHMVIA
                     TQRPSVNIITGVIKANFPARVAFRVSSMMDSRTILDMPGANQLVGRGDMLFSQGNELI
                     RIQCAFVDTPEVENIVNYISKQQSYPTAYYLPEYIKTEGDISMGSIDMSKRDPLFEEA
                     ARLIVANQQGSTSLIQRKFSIGYNRAGRIIDQLEVAGIIGPYEGSKARQVLVADEYQL
                     EQVLKHL"
     gene            5191..5832
                     /gene="nth"
                     /locus_tag="PLR72_05815"
     CDS             5191..5832
                     /gene="nth"
                     /locus_tag="PLR72_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOK36750.1"
                     /translation="MTTEERYQHILKWFSENMPNPQTELHYNNPFELLVAVILSAQCT
                     DKRVNMVTPQLFAAYPTPEAMAKASPEDILKYIQSISFPNSKAQNLVAMAQKLVSDFG
                     GKVPDDVEQLQTLPGVGRKTANIIAAVIYNKPVMPVDTHVFRVAERLGLTTNSKNPLE
                     TEQELVKHIPPELLPKAHHWFILHGRYVCVARKPKCEQCGLKEWCRYYSEHSK"
BASE COUNT         2025 a         1049 c          994 g         2064 t
ORIGIN      
        1 aaagtttaaa tgtttcttct gcttccgtgt ctcgtaatac ttctaaaaaa cttttcgata
       61 cttcctctta tttattcaac tacagttttt cagaaccaca agcctatgca acttaccttg
      121 caacaacacc tgtatttgta gcaacagatg ataaagtagt aggtcttcat gtgtatggag
      181 atttatcagg aaattcgata gaacttcgat ttactgcagg ttcagaagtc aaatatgtta
      241 agttatgtga tttgaatttc tttggttggg aatttgtaga aaccaaatta gataatttac
      301 aagaaggact gccatatgaa tttacaggtt ttagagtggt tagaaatgag ggaattttat
      361 ctggtaatgg tgaattgtat tttgacaata tgcttcttta tgatgctcct attttattcg
      421 taccagaaat actgaaaggg aaaattcata tttatccaaa cccagtatca gatataattt
      481 atattcaaat agaaaatgaa gaaattccta ctttacaact ttattctctt aatggtaaat
      541 tgttaaaaga aacaaaagct ttagaaatga acattcaagg aattgtacca ggaacatata
      601 ttttaatcat taatataaat cgacaaaaag caagtatgcc tgttattatc aagtaattaa
      661 gtaatttagg ttttttctat ttagaatttg accctacctt agttatagaa actggggtag
      721 ggttaatttt gtttcaacca cctaaggttg tataacaaaa aatgtataaa aatgaataac
      781 cccgaggcaa gcctcgagga attcttttga ttaaaaaatt gttatttttg acctataagt
      841 ccagatttta tacttgattg ctcaggaatc gttcggcttc aatggctgct tgacagccac
      901 ttcctgctgc ggttaccgcc tgtcggtagt gaggatcggc tacatcacct gcagcgaaaa
      961 ttccaggaag ttttgtacat ggtgttcccg caatggtttt aaggtaacca gtttcgtctg
     1021 tttccagcca aggcttgaaa atttttgaat tgggttgatg accaatggca aggaaaaaac
     1081 catcaatttc aatatgaact tgttcttcat cgggttgccc gcgacgtttt actaaatttg
     1141 caccttgaac cactttttct cctgttaatc caaccgtttc gtgttcgaaa agtatttcaa
     1201 tttttggatg atttttcact ctttcttgta agattttaga agcccgcaaa taaggcttcc
     1261 gaacaatcat ataaaccttg ctggcaatat tagccagata aagagcttct tcacaagcac
     1321 tgtcgcctcc gccaaccaca gcaacttttt tatttcgata aaaaaatcca tcgcaagtgg
     1381 cacagacaga aactccagct cctgcaaact tttgctcatc aggtaaacca agatatttgg
     1441 cttttgctcc agtagctata ataatagttt cagctttaat ggttttttca ttatcaacaa
     1501 taactttata aggtcttaaa gaaaaatcga cagcagttac cattccaaat ctcacagcag
     1561 caccaaattt ttctgcctgt ttttgcatct tatccataag ctctgctcca ctgattcctt
     1621 cagggaaacc gggaaaattt tctatttcgt tagtaagagt taactgtcca cctggctcta
     1681 aaccttgata taatactgga gagagattgg ctcgtgccgc ataaatagct gcagtacatc
     1741 cggctggtcc tgaacctatg atcaaacaac gtactttttc agacattttt atcagtaaat
     1801 tttttaaaaa tatttcttaa agatgggctt taatttattt tgaagattta tctcaaatcg
     1861 ttaatataaa catcattata ttttgaagga tgaaaaacaa aaaaatcatc attgattttg
     1921 atgttttttt tgaaattaat taatgtcaat agagaagttg aaccgttttt ttcgtttatt
     1981 tctaaagaaa ctggatcgcc tgtggttcta ttaactttta attccacttt gagaatggac
     2041 tcattttttg ttttgggaaa catttctatg caatataatt ccgaatagga ttttttacaa
     2101 aactgagctg aaaatttgtt tttataagcc gataaaaaag taatcggatt ggtatttatc
     2161 aattcttcag cggtaggctc tgtaattgta acttcattat tttgctggga ataagcccat
     2221 tgcgtttttc catcatacca cactttcatt tcattcatat caatcatgaa tttgttacct
     2281 tttaaaaaaa aggatccgtt atacgtttga gaatttatat tatttttttc actaatagta
     2341 agtttaaaat ttgaagaaat ggcattattt gtaaaaagat aaaataaatc gtttataatt
     2401 ttctcagcat cagcactttt ttgagcaaaa agactgttga ttgaaattat caatgctgtg
     2461 attgaaaaaa ataattgttt catttccaga ggttttaaaa aattataact tgaaaaagag
     2521 gtattgaaaa gaatttgtta aagatgcttc aaaacttgtt ccaattgata ttcatcggct
     2581 actaagactt gacgggcttt actcccttcg taaggaccaa tgattcccgc cacttccaat
     2641 tgatcaataa tacgtcctgc tcgattatat ccaattgaaa atttccgttg aatcagcgaa
     2701 gtagaacctt gttgattggc aacaattaag cgagcagctt cttcaaacaa aggatctcgt
     2761 ttgctcatat caatggagcc catagaaata tctccttctg tctttatgta ttctggcaaa
     2821 taataagcgg taggataact ttgctgttta ctgatataat taacaatatt ttcaacctca
     2881 ggagtatcaa caaaagcaca ttgaatacga atcagttcgt ttccttgcga aaaaagcata
     2941 tcaccacgac ctaccagttg atttgctcct ggcatatcca ggatggtacg tgaatccatc
     3001 atggacgaaa cgcgaaatgc aactcgtgct ggaaaatttg ccttaataac acccgtaata
     3061 atgtttacag aaggccgttg tgtggcaatt accatgtgaa tgcctacagc tctggcaagt
     3121 tgggcaattc gagcaattgg catttctact tctttgccag cagtcatgat cagatcgcca
     3181 aactcatcaa taatcaccac aatgtaaggt aaatatttat gtcctttttc aggattcaaa
     3241 ttacgcatta taaatttttc gttatattct ttaatgtttc tgacatgggc ttttttaagc
     3301 aaatcataac gattatccat ttcagtacaa agtgattgaa gtgtttctac cactttactt
     3361 gtatcggtaa cgatggcatc actgtcatca ggaagtttgg caagaaaatg ttttgcaatt
     3421 ccgctgtata tattgaattc tacttttttg ggatcaatca aaaccaattt caattcagca
     3481 ggatgttttt tataaagcaa tgaagtaata atggcattta aacctactga ttttccttgt
     3541 ccggtagcac cagctaccag caaatggggc atttttacca gatcaaacat aaagatttca
     3601 ttggtgatgg ttcgcccaaa agcaataggt aattcatatt ttgattcttg aaattttttt
     3661 gaagcaataa cagaatgcat ggatacaatt tgaggatcat tattgggaac ttctatacca
     3721 atagtacctt tccctggaat gggagcaata attctaatac caattgcagc aaggctcaac
     3781 ataatatccg attcgaggtt acgaattttt gaaatacgga ctcctgcttt tggtacaatt
     3841 tcgtaaagtg taatggtagg accaacggtt gcctttatgg attctatctc aatgtcaaaa
     3901 ttttgtaagg tactgataat tttttcttta ttggcaattt gttcatccat atcgatgggt
     3961 gcattgttat cggtaccgta aactttaagc aaatcgagtg ttggaaaata atagtgagct
     4021 aattccagtg taggatcata tttacctaat ttttcaaaat tataattggg atcagcattt
     4081 tcttcttccc taactcgatc ttcttcttca aggagaggct tgttttcgtc agttgtttca
     4141 actggatata tttcgaatgg aacgtcactt ttctcttgtc cgctaaaata ctcattgctt
     4201 tcatctactc cttttgacaa ccactcttca ggaactattt ctgtgccaat agcaacattt
     4261 atattttggt caggagaaag ctgttttttt tgtttttttg aaaaaatttg tttaagaaaa
     4321 ggaagagcac tattgaacga aattatacaa tatatgagca aagtaccaat gattaccatt
     4381 aatgttcctg gtaccccaat ataggaagtc aaccattgac tggcataatc gccatgctga
     4441 ccacccaatg agaaaaaaag tacattgtta taaaaaggtg aaaaaacaaa ccctaaagta
     4501 accgatagcc agatcaacca gaaagcacaa tgaaaaaaag ttttccacag agatagttta
     4561 actgccttca ttaaacgaag ggctaccact attccaaaaa aaagaataaa aaaggaagaa
     4621 acgccaaacc atcgattaat aagagtttca gccgtaattg ccccgagcac agaagtccag
     4681 ttttgaatag aattccgcat ctgatgtaat tcgctccatg aaaggtcaat tttactttga
     4741 tcagcatatc cggtaaaaaa gtaagaaaca aaagccaata aaaaataaag catgatcaac
     4801 aaaaaaagta accccaaaat aaaatgagtt cgttcatcat taataaaaaa atttattttt
     4861 ttccaaaaac ttactttatt ctcttttttt ggagaggaag aatgagtttt tgaggtggat
     4921 ttgctgctat tttttatcgg ttttttaggt gccatttaat aataaattaa actttaagga
     4981 tttattcaaa aaataataac tttctttttt cttttctaaa tttattcatt tcttttgcaa
     5041 aaataacaaa aagagagatg aaaaaccatt ttttggttta aatttgcaag gttgaaaata
     5101 tttcgcaaaa gaatgatttt tttttgatat tttcaagaac aacaagcaat atttgaataa
     5161 ttcttatttt tcttaaaact tcaaataaaa atgaccacag aagaacgtta tcagcatatt
     5221 ttaaaatggt tttctgaaaa catgcccaat cctcaaacag aattacatta caacaatcct
     5281 tttgaattgt tggttgctgt aattctttct gcccaatgta cagacaaacg agttaacatg
     5341 gtaactcctc aactatttgc tgcttaccct acccccgaag ccatggcaaa agcttctccc
     5401 gaagatattt taaaatatat tcaaagcatt tcttttccta acagcaaagc acaaaacctg
     5461 gtggcaatgg ctcaaaaatt agtttcagat tttggaggaa aagtccccga tgatgtggaa
     5521 caattgcaaa ctttaccagg tgtcggacgg aaaacagcta acattattgc tgctgtgatt
     5581 tacaataaac ctgtgatgcc agtagatacc catgtattta gagtcgctga acgtcttgga
     5641 ttgacaacaa attcaaaaaa tcctttggaa actgagcaag aactggtaaa gcatattcct
     5701 cccgaacttc ttccaaaagc tcatcactgg tttattttac atggacgtta tgtttgtgtg
     5761 gctcgcaaac ctaaatgtga acaatgtggt ttgaaagaat ggtgccgata ttattccgaa
     5821 cattccaaat aaaaatgtga atatattctt ctcttgctca acattaatta tggtaggatt
     5881 tggtataaac aaatctttga ttgtcaaaat caataatctc aaatttgtca ttaagtgaaa
     5941 actttctaat aaaggttgtt ataaaaattc tttataatta tttgaatatc aatataatat
     6001 attgataaaa tgttgaataa attgaggtcg tatttttaat aaaagaagaa ttttttctca
     6061 ttaaaggcag atgaagtttt tttacaaaca aaataatgac cgtgattttc tgcatttaat
     6121 aaaatattat tt
//
LOCUS       DAOSAS010000109         6010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_8362_length_6010_cov_15.290848, whole genome shotgun sequence.
ACCESSION   DAOSAS010000109 DAOSAS010000000
VERSION     DAOSAS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8362_length_6010_cov_15.290848"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2473
                     /gene="cas10"
                     /locus_tag="PLR72_05820"
     CDS             <1..2473
                     /gene="cas10"
                     /locus_tag="PLR72_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HOK36751.1"
                     /translation="RQQIYLGALLHDIGKFYQRYDANSASKSELLDDAIKKLESVYCP
                     NYNNYFTHKHVLWTAQFFDNHREKINKIFGLSKDDDTLMTLAASHHSPDPRNKWALII
                     QQADILSSAAEREEKKQDFKDDEQGWDGFKKIPLKSIFDLISLDGNYPDCAINPIETK
                     PLTINKLPVLNNSEYNPDYGIWEAFNEEFCKIPDTLSPDNFIVTLDALLHRFTYCIPS
                     STVDEPDISLYDHLKTTAFLSVALFDYYSENENLTIPLADTCKPFVLLGGDISGIQDF
                     IYDIPSEGAAKQLKARSFYLHIITETIVRNILKDFNLLQINVVYSSGGNFYILVPNLK
                     ELDDKIQNLNRRINEQLRRVHGTDIYLAMGYNEISIKNLKQKECSNSLNSEKCLSQIW
                     DDLISEKIFKVKNQRYLSSIKEKYDEYFEPTSNFGKQSQDEKFLSKLELLGKNLRGTQ
                     FIVYSSYKHPNIKEEYCFDTKMGFYIYLLNENPAEKFEKSEIIKLNDTNIVNDIKNDN
                     TYRFEFYGGNKYPVGENGYPKTFDKLAEGKNLDRLGIVRMDVDNLGKIFAEGFNPRLR
                     TFSRLTALSRSLDLFFKGYINTIWDRDEFKNDTYILYSGGDDLFIVGRWDKTIEFAYE
                     VKKAFFEFTKSTEFGLSGGVSIEKSKFPIRRAAYNAGKFEGMAKEFRIKSENIDKNYS
                     KPYQKNAFTLFNTALSWQSDFEKVLTMKNDIKKHLFEKQNVSHSFFNSIKLFYMMQQE
                     QTKKELPESWRWTAAYYLQRAKERLGKNNQSFINYIDDLKISMFTDRYRVNQHNEPLN
                     SNYSFLQLLAIAVRWAELELRTEGK"
     gene            2508..2927
                     /gene="csm2"
                     /locus_tag="PLR72_05825"
     CDS             2508..2927
                     /gene="csm2"
                     /locus_tag="PLR72_05825"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HOK36752.1"
                     /translation="MAEHYQPRDQKKAPFKDQFKSEWITKSFDRDADKFAEKFGCFLK
                     EVQLSSSQIRNIYGEIKNLQMRISTDIDFEREKGRFILTKAKMAYTAGRNKSEGLENF
                     RTYFDQAHKEVHNKETFDRFVNFITAVLAYHRAAGGK"
     gene            2957..3628
                     /gene="csm3"
                     /locus_tag="PLR72_05830"
     CDS             2957..3628
                     /gene="csm3"
                     /locus_tag="PLR72_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009012138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HOK36753.1"
                     /translation="MSNNTSYSLVKKVKISGSIKLITGLCIGGTDTGMEIGGLDKTII
                     RNPLSGKPYIPGSSLKGKLRAMLEIAEGAIMHKKMGKVEYIGSDDIKYITTRLFGNSK
                     GDETQRPSRLIVRDCELIEESFGGKQLDLPYAEAKTEVVIDRITAQAMPRTIERVPAG
                     AEFRLEMILNLFNDNGTVDNEKEYKEAIEKAMKLLENDYLGGNGSRGYGQIKFQNIKF
                     QEVEI"
     gene            3645..4745
                     /gene="csm4"
                     /locus_tag="PLR72_05835"
     CDS             3645..4745
                     /gene="csm4"
                     /locus_tag="PLR72_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm4"
                     /protein_id="HOK36754.1"
                     /translation="MKFKIYKLKFTSPLHIGRSSIDDYSKSEEFIHSDTLKSAIFSTA
                     LVLNPELNNPERGKKFFNSFLVSSTFPFYKDYLFLPKPKGKLPLTDIQNKEPSYDFKK
                     KVKKVSFIDFDLFFNFFNNKNQELNFKDDFEIVSNFIVKKKFAEEIDLFLNSNQKNDK
                     GVINFTIVKSSLQERVYIRKTDETYEKSEKKYIYSDPYIVDRINFIKDSGLYFFLETK
                     DEFDINIFEKILNHLGDFGIGTDRNVGNGQFIIDEILDKEIIFDSKADYLMSLSLFLP
                     SEKDVQNFTEKCQYKLIKRGGWISNHIDLNLLKRSQIKKSIYMIDEASVFENNCTLEG
                     TIVDLSPDDNTLNHPVWRDGRPIFLPINNINV"
     gene            4751..5947
                     /gene="csm5"
                     /locus_tag="PLR72_05840"
     CDS             4751..5947
                     /gene="csm5"
                     /locus_tag="PLR72_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HOK36755.1"
                     /translation="MAEFIAKTLTAVHVGSGNKLVKNFDFFTFKDEEDYDCLAIIDVN
                     KVVNVIGINNIEKLINFIDKKGQKNIYSFINESANKKFIIDDISKRQVRVFGDFVNKT
                     ELKEQYLNSDNYPVIPGSSLKGAIRTAIARELVLKNKDYYKRNIISKINQIKNTLSNQ
                     EGKIKTKHFQNIQSQIINTVFSGSYRADANKNTMRFLTICDIVFTYETQATVLDIINY
                     TANGWNPKQGQSAIIETISEGCESEPFRITINNNLLKLNKNFNGNSYLEAESSFLNDI
                     KNIFKIVNDHTKYLVENEIGLLQNILETEKVDRSLEKYFDRLSDVLDQINNTKEDEMV
                     IRVGGNTGWFSITGGWAKEILDEENWQVLYHLLNKNRSVKYFPKTRKTDEYGEVFGFL
                     KIVPKK"
BASE COUNT         2326 a          741 c         1035 g         1908 t
ORIGIN      
        1 acgtcagcaa atatatttag gtgctcttct gcatgatatt gggaagtttt atcaacgtta
       61 tgatgctaat agtgcatcta agagtgaatt gctagatgac gctattaaaa aattagaaag
      121 tgtttattgc cctaattata ataactactt tacacataaa cacgttctat ggactgctca
      181 gttttttgat aatcatcgtg aaaaaattaa taaaatattt ggattaagta aggacgatga
      241 tacattaatg acattagcag cttcgcatca ttcacctgac ccaagaaata agtgggcttt
      301 aataattcaa caggctgata ttttatcgtc agcggctgaa cgggaagaaa aaaaacaaga
      361 ttttaaggat gatgagcagg gttgggatgg gtttaagaag atcccattaa agtcaatttt
      421 tgacttaatt tcgcttgatg gaaattatcc agattgtgct atcaatccca ttgagacaaa
      481 accattaact attaataaac tacctgtatt aaataattct gaatacaatc ctgattatgg
      541 aatatgggaa gcatttaatg aggaattttg taaaatacct gatacattaa gtccagataa
      601 tttcatagtt actttagatg cattacttca tagatttaca tattgtattc catcaagcac
      661 agttgatgaa cccgatattt cgctttacga tcatttgaaa actacagcct ttctttctgt
      721 tgctttattt gattactatt cagaaaatga aaatttaaca attccgcttg ccgatacttg
      781 taaaccattt gtcttattag gcggagacat ttctggaata caggatttta tttatgatat
      841 ccctagcgag ggtgcagcaa aacaattaaa agcacgctct ttttacttgc atataattac
      901 tgaaacaatt gttagaaata ttcttaagga ttttaattta cttcaaataa acgtagttta
      961 tagttcaggg ggcaactttt atattttagt tcctaattta aaggaattag atgataaaat
     1021 acaaaatctt aaccgaagga taaatgaaca actccgtcgt gtacatggta ctgatatata
     1081 ccttgcgatg ggttataatg aaatatcgat aaaaaatcta aaacaaaagg aatgttcaaa
     1141 ttccttaaat tctgaaaaat gtttaagtca aatttgggac gatttaatat cagaaaagat
     1201 ttttaaagtt aaaaatcaga ggtatttgtc ttctataaaa gaaaaatacg atgaatattt
     1261 tgaaccaaca tcgaattttg gaaaacaaag tcaagacgaa aaatttttaa gtaaattaga
     1321 gttgttaggg aaaaacttaa gaggcaccca atttattgta tattcttcct ataaacaccc
     1381 aaacataaaa gaagaatatt gttttgatac aaaaatgggt ttttacattt atctattaaa
     1441 tgaaaatccc gctgaaaaat ttgaaaaatc tgaaattatt aagttaaatg atacaaacat
     1501 agtaaacgat attaaaaacg ataatacata taggttcgaa ttttatggtg ggaataagta
     1561 ccctgtagga gagaatggat atcccaagac ctttgacaaa ctagcagaag gtaaaaactt
     1621 agatcgttta ggtatagtaa gaatggatgt tgacaattta ggaaaaatat ttgctgaagg
     1681 atttaatcca aggttgcgga cattttctcg gcttactgca ctaagtcgga gtctcgacct
     1741 tttcttcaaa ggatatatta atacaatttg ggatcgggat gaattcaaaa acgataccta
     1801 tattctttac tctggaggag atgatttgtt cattgtaggt cgctgggata aaactattga
     1861 atttgcatat gaagtaaaaa aagctttctt tgaatttaca aaatctacag aatttggatt
     1921 atcaggtggg gtttcaattg aaaagtcaaa gtttcctata cgcagagctg catataatgc
     1981 aggaaagttc gaaggaatgg caaaggaatt tcgtattaaa tcagaaaata ttgataagaa
     2041 ttattctaaa ccttaccaaa agaatgcttt tacattattt aacactgctt taagttggca
     2101 aagtgatttt gaaaaggtat taaccatgaa gaacgacata aaaaaacatt tatttgaaaa
     2161 gcaaaatgtt tcgcattctt tttttaattc aattaagttg ttttacatga tgcaacaaga
     2221 acagacaaaa aaagaattac ctgaaagttg gcgttggact gctgcatatt atttacaaag
     2281 agcaaaagaa cgtttaggca aaaacaacca aagtttcatt aattacattg atgatttaaa
     2341 gataagtatg tttactgatc gatatcgagt taatcagcac aacgagcctt taaattccaa
     2401 ttattcattt ctccaactcc ttgccatagc agtccgctgg gcagagttgg agttgagaac
     2461 agaaggaaaa taaattataa atactaataa caaaaaaaat taacaatatg gcagaacatt
     2521 atcaacccag agatcaaaag aaagcacctt tcaaagatca gttcaaatcg gaatggataa
     2581 caaaaagttt tgatagggat gcagataagt ttgcagaaaa atttggttgt tttttaaaag
     2641 aggtgcaact ttcatcaagt caaattagaa atatttatgg tgaaataaag aatcttcaga
     2701 tgagaatttc aaccgatatt gattttgaaa gagaaaaggg aagatttatt cttacaaaag
     2761 caaaaatggc atatacggct ggcagaaata aatctgaagg tcttgaaaat ttccgaacat
     2821 attttgatca agcccataaa gaggttcata ataaagagac atttgatcgc tttgttaatt
     2881 tcattactgc tgttttggca tatcatagag cagcaggggg aaaatagttt aaagtataac
     2941 ttaaaatatt aaagccatga gtaacaatac atcctatagt ttggtaaaaa aagtaaaaat
     3001 ttctggctct attaaattaa taaccggttt gtgcattggg ggtaccgata ctggtatgga
     3061 aattggaggt cttgataaaa caatcataag aaacccttta tcgggtaagc cctatattcc
     3121 tggaagttca ttaaagggaa aacttagagc tatgcttgag attgctgaag gtgcaattat
     3181 gcataaaaaa atgggtaaag ttgaatatat tgggagtgat gatattaaat atattacaac
     3241 ccgtttattt ggtaattcaa aaggtgatga aacgcaacga ccgtcacgct taatagttcg
     3301 cgattgcgaa ttaatagaag aaagttttgg tggaaaacag cttgatttac cctatgccga
     3361 agctaaaacc gaagtagtta tcgatagaat tactgctcaa gccatgcctc gtacaattga
     3421 acgagttcct gcaggagctg aatttagatt agaaatgatt ttaaaccttt ttaatgataa
     3481 tggcacggta gataatgaga aggaatataa agaggctatt gaaaaggcaa tgaaattact
     3541 tgaaaacgat tatctgggag gaaatggttc acgaggatat ggtcaaatca aattccagaa
     3601 tatcaaattc caggaagtcg aaatctgatt aaccatttat taaaatgaag tttaaaatat
     3661 ataaattaaa atttacttcg ccgctccata taggccgaag cagtatagat gattacagca
     3721 aatcagaaga attcatacat tccgatacgc taaaatcggc aatattctca acggctttag
     3781 ttttaaatcc tgaattgaat aatccagaaa gaggtaaaaa attttttaat tcttttttag
     3841 tgagttcaac tttcccattt tacaaggatt atttgttttt accaaagcca aagggcaaac
     3901 tacctttaac agatattcaa aacaaggaac ccagttatga ttttaagaaa aaagtaaaaa
     3961 aagtaagttt tattgatttt gatttatttt ttaatttttt taataataaa aatcaagagt
     4021 taaattttaa ggatgatttt gaaatagttt caaattttat tgtaaagaaa aaatttgctg
     4081 aagaaattga tttattttta aatagtaatc aaaaaaatga taaaggagtg ataaatttta
     4141 caatagtaaa atctagtctg caagaaagag tctatattcg aaaaacggat gaaacttatg
     4201 aaaaaagtga aaaaaaatat atctattcag atccttatat agttgataga ataaatttta
     4261 ttaaggattc tggtttatac ttctttttag aaacaaaaga tgaatttgat attaatattt
     4321 ttgaaaaaat attaaatcat ttaggtgatt ttggtatagg aacagataga aacgttggga
     4381 atggtcaatt tattattgat gaaattttgg ataaagaaat aatttttgat tcaaaagcag
     4441 attatttaat gtcgttgtcc ttgttcttac cttcagaaaa agatgttcaa aattttactg
     4501 aaaagtgtca gtataagctt ataaagcgag gtggatggat cagcaatcat atagatttaa
     4561 atttgttgaa gagaagtcaa atcaaaaagt ctatttatat gattgatgaa gcttcagttt
     4621 ttgaaaataa ttgtacgtta gaaggaacaa ttgttgattt gtcaccagat gataatacct
     4681 taaatcatcc ggtttggaga gatggcagac cgatattttt accaatcaat aatattaatg
     4741 tttaataaaa atggccgagt ttattgcaaa aacacttaca gcagttcatg ttggtagtgg
     4801 aaataaatta gtaaaaaatt ttgatttttt tacttttaaa gacgaagaag attatgattg
     4861 tttggcaata attgatgtaa ataaagtagt aaacgtcatt ggaattaata atattgagaa
     4921 gttaatcaat tttatcgata agaaaggtca aaagaatatt tattctttta ttaatgaaag
     4981 cgcaaataaa aaatttataa tagatgatat ttcaaaaagg caagtaagag tttttggtga
     5041 ctttgtgaat aaaacagaac ttaaggaaca atatttaaac tctgataatt atccagttat
     5101 accaggaagt tcattaaagg gtgctataag aactgcaata gcaagagaat tagttttaaa
     5161 aaataaagat tattataaac gaaatattat tagtaaaatt aaccaaataa aaaatacgtt
     5221 atcaaatcaa gagggtaaaa taaaaacaaa acattttcaa aatatacaat cacaaataat
     5281 taatacagtt ttttctggaa gttatagagc cgatgccaat aaaaatacaa tgcgtttttt
     5341 gactatttgt gacatagtgt ttacatatga aactcaagca acagtattag atattattaa
     5401 ttatacagca aatggttgga atccaaaaca gggccagagt gcaataatcg aaactatttc
     5461 ggagggttgt gaatctgaac cttttagaat tacaataaac aataatcttt taaaactaaa
     5521 taaaaatttt aacggtaata gttatcttga agctgaatca tcttttttaa acgatataaa
     5581 aaatattttt aaaatagtga atgatcatac aaagtatctt gtcgaaaatg aaattggttt
     5641 attacaaaat atattagaaa ctgaaaaagt tgatagaagt ttagaaaagt attttgacag
     5701 gcttagtgat gtgttagatc agataaataa tacaaaagaa gatgaaatgg taatacgtgt
     5761 tggtggaaat accggttggt tttcaataac aggtggatgg gcaaaagaaa ttctggatga
     5821 agaaaattgg caagttttat atcatttatt aaataaaaat cggagtgtta aatattttcc
     5881 aaaaacccga aaaactgatg aatatggtga agtttttggt tttttaaaaa tcgttcctaa
     5941 aaagtaatat gaaaatccac atcaccctcg ttggaggaca aaaagagcct gtatatcaag
     6001 gcattgttta
//
LOCUS       DAOSAS010000110         5966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_8443_length_5966_cov_10.491118, whole genome shotgun sequence.
ACCESSION   DAOSAS010000110 DAOSAS010000000
VERSION     DAOSAS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8443_length_5966_cov_10.491118"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLR72_05845"
     CDS             complement(<1..289)
                     /locus_tag="PLR72_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36756.1"
                     /translation="MKKLTFLKSLLLGILFSFSANMYALSVGDIAIIAVNTDAPKTFT
                     FVALADIPANTTISFTDNAWNATTSTWRTGEGTIQWSYTAAVTAGTVITLTV"
     gene            complement(704..2413)
                     /locus_tag="PLR72_05850"
     CDS             complement(704..2413)
                     /locus_tag="PLR72_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOK36757.1"
                     /translation="MKKLTFKTSLLLALFFLWGSGIKAETYYVDFESLTKTSYASGTV
                     TLGAYDWNFTGACAGNLDSDWKVGSVSARLAGTTKASSNSETSKIEMLSNKAGGIGNI
                     SFQYRHYGSDAQIPWLVQWSSDGSTWTTIQEITGTEQVQTFSYDLNQDNARIRIIANG
                     FTTSDANGKRLNVDELILTDNSGTPVPTITVTETTLEGFSYEVGKGPSAEKSFQVSGN
                     NLTGNITLTPSDNLEISTGSGENFVATSPINLPPTEGVVAATSIYARLKAGLSAGNYT
                     ETITATSPGAVTKTITCKGNVFEPVGTVPNVIITEVYGGGGNSGAELKNDFIELYNTT
                     DTPVDITGWSLQYYSATGTGKAADGNVFKILDDKSIPAYTHFLIQCAAGNSGTQDLPT
                     PDATCELALSGTSGKVILYTTSEPQAITTDITSITGNPYFKDYVPYGTTAVPVWGTPM
                     SSNTSNTTSAARKIISDATPRIYQYTGNIGNDFEVATPTPQNTEWTSGIKNVFENLNV
                     YVADGKIHINTTTVGEQIEVYNTLGQRLVNVLSTGEENIISVNAKGVLLVKCGNKVGK
                     VIL"
     gene            2720..3334
                     /locus_tag="PLR72_05855"
     CDS             2720..3334
                     /locus_tag="PLR72_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOK36758.1"
                     /translation="MNVDELPDHVVYSQKVIEFVTVAAETCLFLEHASEIDKHDFVDK
                     SLKLLPLLYLKASLIEIPEPVYDEEPERFVTEEDYQFVRQQVEQLLGNEDAYLEVFHP
                     DMSYSDTPVITFISENLADIYQEIKDFAANYQLADVDIMNDALVTCLVTFAEHWGQKL
                     LNAMRALHALKYTEENKLEKEIDEEKDTHKISRDSFLDFQRKTE"
     gene            3341..3985
                     /locus_tag="PLR72_05860"
     CDS             3341..3985
                     /locus_tag="PLR72_05860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOK36759.1"
                     /translation="MKYSPKISKEEINKLPVATFTGKITVVDESSKVESSIAELRKND
                     IVGIDTETKPSFIKGVSHKVSLLQIATPEQCFLFRLNRIGFPKALADFLADKNITKVG
                     LSLHDDLRKLKKRHHFKPENFIDIQSIVKNYGIMELSLQKIYAIIFGEKILKSQKLSN
                     WEKPQLTEKQLQYAAIDAWATLQIYLQLMKEEPLAKKEIEKLVLENSPAKNGSN"
     gene            4013..5200
                     /locus_tag="PLR72_05865"
     CDS             4013..5200
                     /locus_tag="PLR72_05865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOK36760.1"
                     /translation="MITVRLKPKKEESVKRFHPWIFSGAIQSIEGNPEEGDMVKVFDA
                     QNNFLGIGHYQKGSIAVKIFSFEDVEINESFWFSKIQKAFYLRQSLGIIQPSSNNTYR
                     LIHGEGDFLPGLIIDVYGDTAVMQAHSIGMHHVRHILAKAVIKTVPDVSNIYYKSMST
                     LPHKADISDKEGYLMGDGTKNHIILENGLKFYVDWFEGQKTGFFIDQRENRSLLERYS
                     NGKSVLNMFCYTGAFSVYALRGNARLVHSVDSSSKAIEITNKNVALNFPNEIRHQSFV
                     SEAFDYFHQFDNQSSPYNLIILDPPAFAKHQHSIQNALRGYKHLNSEALQIIQPGGIL
                     FTFSCSQAISKEQFRLAIFSASAESKRKVRILHQLSQPMDHPINIYHPEGEYLKGLVL
                     WVE"
     gene            5358..5951
                     /locus_tag="PLR72_05870"
     CDS             5358..5951
                     /locus_tag="PLR72_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK36761.1"
                     /translation="MESLHQLTDDKLVKLYKEGNNKAFEVLLSRYQSKVYTYIYLIVR
                     NKELAEDIFQDTFIKAIATIQQDRYVESGKFLAWVNRIAHNLIIDYFRREKNESTFSI
                     EGMATDLLNNAKFSEKSTEDIILKEQILVDVIRLVDELPLTQQMVVKMRFFENLSFKE
                     IAEKTNVSINTALGRMRYALINIRRLARKKNVSLEIK"
BASE COUNT         1987 a          991 c         1048 g         1940 t
ORIGIN      
        1 taacagttaa tgttataaca gtaccagctg ttaccgcagc tgtataagac cattgaatag
       61 tgccttcacc agttctccac gtagaagtag tagcattcca tgcattgtca gtaaaactaa
      121 tagtagtgtt agcaggtata tctgctaaag caacaaaagt aaatgttttc ggagcatcgg
      181 tgttcactgc aatgatagca atatcaccta cacttagggc atacatatta gcactgaaag
      241 aaaacaatat gcccagcagc aacgatttca aaaaagtaag ttttttcata acggtaaatt
      301 tttaaaagtg aaacattgtg ttattttaaa aaagaatttt tacaaaatta aaaaagaaaa
      361 ccaactgtgt ctttactttt ctgtgaaaaa gctattaaaa aagacttgtt ttctctgtcg
      421 agaatgaaaa tgagcaataa aagtaaagca tatttttata cttggcaaat aaatattcaa
      481 tttttttcag tattttttta ttttattttt gttggtgtaa acttttgtct gttccttgct
      541 ctggaaaagc aaaaaattca ttcaatattc cggtatttaa tttcctgtat gccacccgat
      601 acaatggttc ggcaatagaa gatgattgca gtaagcaaga aaaaagctgc ccgaaaaacg
      661 agcagctttt tacctaccat gaaaaagtta cctgcttata aagttataaa ataactttcc
      721 caactttgtt gccacatttt accaaaagaa ctcctttggc gttgacagaa ataatatttt
      781 cttctcctgt ggaaagaaca ttaaccaatc gttgacctaa ggtattgtac acttcaattt
      841 gttctccaac tgttgtggta ttgatgtgga ttttcccgtc ggctacatac acgttcaagt
      901 tttcaaaaac attttttata cctgatgtcc attctgtatt ttgaggagtg ggggtagcga
      961 cctcaaaatc atttccaata tttccagtat attgatagat acgaggagta gcatcagaaa
     1021 tgatttttct tgcagccgaa gtagtgttag aagtattact actcatgggt gttccccaaa
     1081 ctggtacagc agtagtccca taaggaacat aatctttaaa atatggattt cctgtaatgg
     1141 aagtaatatc agtggttatc gcttgaggct cggaagtagt ataaagtatt actttgcctg
     1201 atgtacccga aagagctaat tcacaagtag catcaggagt aggcaaatct tgcgttccac
     1261 tattacctgc tgcacattgt atcagaaaat gagtataagc aggaattgat ttgtcatcta
     1321 gtatcttaaa tacatttccg tccgctgctt tgcctgtgcc tgttgcggaa taatattgga
     1381 gactccaacc tgttatatct acgggtgtat cggtagtatt gtacaattca ataaaatcat
     1441 ttttcaattc tgcacctgaa ttacctccac cgccatacac ttctgtaata atcacattag
     1501 gaacagtacc tacaggttca aaaacatttc ctttgcaggt gatggtttta gttactgctc
     1561 ctggagaagt agctgtaatg gtttcagtgt aatttcctgc agacagtcct gcttttaagc
     1621 gagcataaat agaagtagca gcaacaactc cctctgttgg aggtaagtta ataggcgaag
     1681 tagcaacaaa attttctccg ctgcctgttg aaatttcaag attgtctgag ggtgttaagg
     1741 taatattacc cgtaagattg ttcccgctta cctggaatga tttttctgcc gaaggtcctt
     1801 tccctacttc ataagaaaat ccttccaacg ttgtttcggt tacggtaatg gtggggacag
     1861 gagtgcctga gttgtcagtc aatatcaatt catccacatt cagacgtttt ccgttagcgt
     1921 cagaagtagt aaaaccattg gcaataattc ggatacgtgc attgtcttga ttcaaatcat
     1981 aagagaaagt ttgaacttgt tcggttccag taatttcttg aatggttgtc caagtactgc
     2041 catcggagct ccactgaacc agccagggaa tttgtgcatc gcttccataa tgccgatatt
     2101 ggaaagaaat attcccaatg cctcctgctt tgttggagag catttcaatt tttgaagttt
     2161 ctgaattgct ggaagcttta gtggtaccag ccaatcgagc agagacactt cccactttcc
     2221 aatcactgtc tagattacct gcacaagcgc cagtaaaatt ccaatcgtaa gctcccaaag
     2281 ttacggttcc tgatgcataa gaagttttgg ttaagctttc aaaatcaaca tagtacgttt
     2341 cagcttttat gccactaccc cagagaaaaa atagagctaa tagcagactt gttttaaaag
     2401 taagtttttt cataattttt ttgttttaaa tatttgacat tctatgaaaa ctacaagaaa
     2461 ttttcttatt gttgaaaatt attgaatatg aaaatttcaa ttgaaataaa aggaacttca
     2521 aatgtaaaat aatttttgta aaaaaagaaa taattattca atttattttt ttattgaaca
     2581 aatggtttca aactcgcatc ccgcaacttc gagaactcca aactattgac ttatttttat
     2641 tttatttata atggcaacct gttataaatt gaaaattatc ttgtaaattt gttttgaaat
     2701 aatcattaaa aactgtttta tgaatgttga tgaattaccc gatcatgttg tttatagtca
     2761 aaaagtgatt gaatttgtga ccgttgcagc cgaaacctgt ctttttttag agcatgcttc
     2821 tgaaatcgac aagcatgatt ttgttgataa atcattgaaa ttattgcctt tgctatattt
     2881 gaaagcatcg ctaattgaaa ttcctgaacc tgtttatgac gaagagcccg aaagatttgt
     2941 aaccgaagaa gattatcaat ttgtccgtca acaagtggaa caacttttag ggaatgagga
     3001 tgcgtatctg gaagtttttc atcccgatat gtcttacagt gataccccag taattacctt
     3061 tatttctgaa aatcttgctg atatatatca agaaatcaag gattttgctg ccaattatca
     3121 actggctgat gtggacataa tgaatgatgc gttggttact tgtctggtta cttttgcaga
     3181 acattgggga cagaaattat taaatgcaat gcgagcactt catgctttaa aatatacaga
     3241 agaaaataaa ttggaaaaag aaatagatga agaaaaagat acccataaaa tcagtcgaga
     3301 ttcgtttctt gattttcaac gtaaaacgga ataataaccg atgaaatatt cacctaaaat
     3361 atctaaagaa gaaattaata aattacccgt tgctactttt acaggaaaaa ttactgttgt
     3421 agatgaatct tcaaaagtag aatcgtccat tgctgaactt cgtaaaaatg atatagtagg
     3481 aattgataca gaaacaaagc cttcttttat caaaggcgtt tcccataaag tttcactttt
     3541 acaaattgct actcccgaac aatgtttttt attccgacta aatcgaattg gttttccaaa
     3601 agctcttgct gattttttag ctgataaaaa tatcaccaaa gttgggcttt ctctgcatga
     3661 cgatttaaga aaattaaaaa aacgtcatca ttttaaacct gaaaatttta ttgatattca
     3721 atctatagta aaaaactatg gcattatgga attaagcttg cagaaaattt atgctatcat
     3781 tttcggagaa aaaattctga aatcgcaaaa actaagtaat tgggaaaaac cacaactgac
     3841 tgagaaacaa ttacaatatg ctgctattga tgcttgggca actttgcaaa tctatctcca
     3901 attaatgaaa gaagaaccgt tggcgaaaaa agaaatagaa aaattagtac tggaaaattc
     3961 acctgctaaa aatgggtcca attaattcat tttaaaattc aattcctatt ttatgattac
     4021 tgtgcgattg aaaccaaaaa aggaagaaag tgtgaaacgt ttccatccgt ggattttctc
     4081 aggtgctatt cagagcatag aaggaaatcc tgaagaaggt gacatggtga aagtttttga
     4141 tgctcaaaac aattttttag gtatcggaca ttatcagaaa ggcagtattg cagtaaaaat
     4201 attttcgttt gaagatgttg aaataaatga atcattctgg ttttcaaaaa ttcaaaaagc
     4261 tttttattta cgccaatcat tggggatcat tcaaccttcc agtaataata cctatcgttt
     4321 gattcatggc gaaggcgatt ttctgccagg actaatcatt gacgtttatg gtgatacggc
     4381 tgttatgcaa gcacattcta tcgggatgca tcatgttcgt catattttgg caaaagctgt
     4441 aatcaaaacg gttcctgatg tttcaaatat ctattacaaa tcaatgtcaa ctttacctca
     4501 taaagcagat atttctgata aagaaggtta tttaatggga gacgggacaa aaaatcacat
     4561 tatactggaa aatggtttga agttttatgt agattggttt gaaggacaaa aaacaggttt
     4621 ttttattgat caacgagaaa atcgttcttt gctggaacgt tattcaaatg gaaaatctgt
     4681 attgaatatg ttctgctata cgggagcatt ttcggtgtat gctttaagag gaaatgcgcg
     4741 attggttcat tcagtggata gttcgtcaaa agcaattgaa atcaccaata aaaatgtggc
     4801 tctgaatttt ccgaacgaaa ttcgtcatca atctttcgtt agtgaggctt tcgattattt
     4861 tcatcagttt gataatcagt caagtcctta taatttaatt attctcgatc cgccagcttt
     4921 tgccaaacat cagcattcca ttcaaaatgc cctaagaggt tataaacatt taaattcgga
     4981 agccttacaa ataattcaac cgggtggtat cctttttact ttttcctgct ctcaagccat
     5041 tagtaaagaa caattcaggc tggctatttt cagtgcatct gctgaaagta aacgtaaggt
     5101 acgcatcttg catcaacttt cacaacctat ggatcacccc atcaatattt atcatcccga
     5161 aggagaatat ttgaaaggtt tggtgttatg ggtagaataa agcttgagaa aaagtagttt
     5221 tttcaaattc tcgacgaaaa aaatttgttt ttttaaaaac aataattata tttgcaactc
     5281 tttatcacac tcaattgttt aactttaaat ttaatgccta ttgctaaaaa ttactcctaa
     5341 tcaataaaat agaagtaatg gaatctttgc accaacttac cgatgataaa ttggtaaaat
     5401 tgtataaaga aggcaataat aaggcatttg aagtgttatt atcacgctat caatcaaaag
     5461 tttatactta tatttatttg attgttcgta ataaggaact tgccgaagat atttttcagg
     5521 atacttttat taaagctatt gctaccattc aacaagaccg ttatgttgag agtggtaaat
     5581 ttttggcatg ggttaatcgc attgcccata atttgataat tgattatttc cgtcgcgaaa
     5641 aaaatgaaag tactttttcc atcgaaggaa tggcaaccga tttactaaac aatgccaaat
     5701 tttctgaaaa aagtacggag gatattattc taaaagaaca gatacttgtt gatgtaatcc
     5761 gtctcgttga tgaattgcct ctcactcagc aaatggtggt aaaaatgcgt ttttttgaaa
     5821 atctaagttt caaagaaatt gccgagaaaa caaatgttag catcaatact gctctcggac
     5881 gaatgcgata tgcccttatt aacatacgcc gtttagcgag aaagaaaaat gtatcgctgg
     5941 aaatcaagta agaaaagata tatagg
//
LOCUS       DAOSAS010000111         5872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_8617_length_5872_cov_14.178271, whole genome shotgun sequence.
ACCESSION   DAOSAS010000111 DAOSAS010000000
VERSION     DAOSAS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8617_length_5872_cov_14.178271"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..342)
                     /locus_tag="PLR72_05875"
     CDS             complement(172..342)
                     /locus_tag="PLR72_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36762.1"
                     /translation="MKKLVLLFAVVFAFVACGKKATQESTETDTTSVVAPVDTIVESI
                     DTTTVDTTVAPM"
     gene            complement(553..723)
                     /locus_tag="PLR72_05880"
     CDS             complement(553..723)
                     /locus_tag="PLR72_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36763.1"
                     /translation="MKKLVLLFAVVFAFVACGKKATEEKAESEDSIVIVDTIESQIDT
                     TNVIDTMATPFE"
     gene            complement(1073..3517)
                     /locus_tag="PLR72_05885"
     CDS             complement(1073..3517)
                     /locus_tag="PLR72_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOK36764.1"
                     /translation="MVYPQNFEQKIDFGNIRQLLKEKSISTLGKEKIDSIHFSSDFYK
                     IIQQLDQTNEMLQILTTETNELPLSNIYDIRPSLHKIHVEGLYLTENELYCLRTNLEN
                     AQLLTTYFSKLNSTFYPQLSRLSKTVKSFSFLIDQINKILTKYGDIKDNASPQLEKIR
                     KNILTTQNNISRTLNEILQRAFSNGYVEKDTLPTLREGRLVIPVSQAFKRKINGIVHD
                     ESASGKTVYIEPAEVIELNNSIRELEGEERREIIRILTDITNYIRPFIPDLLELQNFL
                     GEIDFLHSKALFAIDIKAIRPNVENKCIIQWNKASHPLLYLSLQKQGKNIVPLDIVLN
                     ENQRILIISGPNAGGKSVCLKTVGLLQYMLQCGLLIPLDESSQIGIFENLFIDIGDEQ
                     SIENDLSTYSSHLLNMKFFIQHSNEKTLVMIDEFGSGTEPQIGGAIAEAVLERINQQK
                     AFGVITTHYTNLKHFAENTEGVVNGAMLFDRGRLQPLFQLQIGFPGSSFAIEIARKIG
                     LPDDIIEEAAQKVGKEHFDYEKHLHEIIRDKRYWENKRKKIRQREKYLEKIIEQYETT
                     LNNIEKKQKEIIQATKAEAIQLLNEANAKIENTIRQIKEAQAEKEKTKEIRKELETFK
                     KEKLEPEILPKPIEPSIQLKRKKSKKVASENLSKSTFQVGNYVKIKGHSLVGRIIEIS
                     EEQAIIAFGQMKSTIELSKLEFINRTQTKKEINKIQQSIELKNEEIKNRKLNFKTEID
                     VRGMRGEEAVQAVSYFIDDAIMVGANSVRILHGTGSGVLRQLIRQYLATVPQIKNFHD
                     EHIELGGSGITVVNFE"
     gene            complement(3529..4329)
                     /gene="mazG"
                     /locus_tag="PLR72_05890"
     CDS             complement(3529..4329)
                     /gene="mazG"
                     /locus_tag="PLR72_05890"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOK36765.1"
                     /translation="MEDKKRTLDEIKEEFGRLVEIMNELRQKCPWDKEQTFDSLRVQT
                     IEETYELSDAIMKKDMEEIKKELGDLLLHIIFYAQMANENNDFDIYEVCKALNEKLIY
                     RHPHVFSNVKSDSTETVVQNWEKLKLKEKNGNKTVLGGVPDSLPALIKAHRIQDKARY
                     VGFDWEEKEQVWEKVKEEFLELQQEIKSLDKDKIEEEFGDLLFSLINAGRLYDINPEN
                     ALERTNRKFINRFNYLESKTISQGKDLKQMTLQEMDEIWNEAKKLESK"
     gene            complement(4561..5757)
                     /locus_tag="PLR72_05895"
     CDS             complement(4561..5757)
                     /locus_tag="PLR72_05895"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VLRF1 family aeRF1-type release factor"
                     /protein_id="HOK36766.1"
                     /translation="MISKNDIQRFKTLVSSINEPVLSVYCNVNPAIPENVGRAWYDRL
                     KNTLKSMNELNIKLKKGKSFYEELIIFLDQIRTEARTLVLFAWINEKGELAVEHFELQ
                     VDLPVVDLRSGRVEAHFGKPYLTPILYAFDEFRRVGVLHIYGNKWRFYEVFMNEVKEV
                     TDVFAAITPNEWKEFNEYSQIIESGVLEDKMFNAKMKFKDKIKAKQQTFSHKLYVTLA
                     QMLEKSIHEFEMDRLILMGNDWQIKLFENYLSRPLRQLIVGYATNPANPENPSAKDIL
                     DRISPIMREAEEQEEMQLINEAQGPKGVSGLQNVLEALQMGQLQVLILPWDLNVSVYR
                     CSDGIIFATPEEAKDYDDSFEEIPLKKEIFYLAAKYATRLEFVNNVPKEKLYNELEGI
                     AGILRW"
BASE COUNT         1837 a          981 c          789 g         2265 t
ORIGIN      
        1 aaaaaataaa aacaaaagct accccgacca agcatcgggg aattcttacg attaaaaatt
       61 cttgattaag aaaagatgtg aatttacaaa tttgattttt tacaaaataa aaaagtccgt
      121 tgtagttaaa aaacggactt actattacag aactactttt gttcctaatt attacatagg
      181 agctacagta gtatcaacag ttgtagtgtc aatactttca acaatagtat caactggggc
      241 aactacactt gtggtgtcgg tttctgtgct ttcctgagta gcttttttcc cacaagcaac
      301 aaatgcaaaa actacagcaa ataacaatac taattttttc attttttttt atttttaatt
      361 taacatgtta atgttatatt ctaaaacgat gcaaagttac atcttttttt ttatttaatt
      421 aaataaatgt cattttttta attaaacttc gaaaagttaa ttactcggta taaaatttat
      481 tccttaaaaa ttaaactttt gaaaatagaa tcctctatat gaatggtttt ttattattta
      541 tagtctgaac aattattcaa atggagttgc catagtgtca atgacattcg ttgtgtctat
      601 ttgtgattct atggtatcta caattactat agaatcttca ctttcagctt tttcttcagt
      661 agcttttttc ccacaagcaa caaatgcaaa aactacagca aataacaata ccaatttttt
      721 catttttcac aattttttaa aagattaatt ttttaaccac tattcaaaaa acgatacaaa
      781 tatacaactt ttttgtatat gttgttcata aaacttattt tttagaactt ttttgttgtt
      841 ttgttataaa aaattgattt taatcatttt atcttctaat tcaacggatt gacaaaccat
      901 ttattttatt atttagaaca aaaaataaaa atgaaatgaa aataaatgtt aattaaaaat
      961 aaatatgttt taagataaat tagaaaatgc agaaatttgt ttttgagacc actctttaaa
     1021 aaactatttt tatttttttt aaaaatcaat attgttattc aattttgaat ttttattcaa
     1081 aatttacaac agtaattcca gagccaccta attcgatatg ttcatcatga aaatttttta
     1141 tttgtggaac agtagcaaga tactgacgaa tcaactgccg caatactcca ctacctgttc
     1201 catgtaaaat acgcacagaa ttggctccca ccataatggc atcgtcaata aaataactta
     1261 ctgcttgcac agcttcttct cctctcattc cacgaacatc aatttctgtt ttgaaattta
     1321 gtttcctgtt ctttatttct tcgtttttta attcaataga ttgttgaatt ttgttgattt
     1381 ctttttttgt ttgtgtgcga ttgataaatt cgagtttaga aagttctatt gttgatttca
     1441 tttgaccaaa agctataatg gcttgttcct cactgatttc tataattctt ccaacaagag
     1501 agtgtccttt tatttttaca taattaccta cttggaaagt ggattttgat aaattttccg
     1561 aagcaacttt ttttgatttt ttccttttta attgaatgga aggttctatt ggtttaggta
     1621 aaatttctgg ttccagtttt tctttcttaa aagtttccaa ttcttttctt atctcctttg
     1681 ttttttcttt ttctgcttga gcttccttaa tctggcgtat agtattttca atttttgcat
     1741 tagcttcatt caataattgg atggcttcgg cttttgtagc ttgaataatt tctttttgtt
     1801 ttttttcaat attatttaat gttgtttcgt attgctcgat aattttttcg agatattttt
     1861 ctcgttgacg gattttcttt cgcttatttt cccaataacg tttatcccga ataatttcat
     1921 gcaaatgttt ttcgtaatca aaatgttctt tcccaacctt ttgagcagct tcttcaataa
     1981 tatcgtcagg caaacctatt tttcgagcta tttcaatcgc aaaagaactt ccaggaaaac
     2041 caatttgcag ttgaaaaagt ggttgcaatc gtccgcgatc gaacaacata gctccattta
     2101 ccactccttc ggtgttctcg gcaaaatgtt ttaaatttgt gtaatgggtg gtaatcacac
     2161 caaaagcttt ttgctgatta attctttcca aaacagcttc agcaatggct ccaccaattt
     2221 gaggctcagt accagagcca aattcgtcaa tcataaccag cgttttttca ttgctgtgct
     2281 gaataaaaaa tttcatgttc agcaaatggg aactataagt agaaaggtca ttttcaatag
     2341 attgttcatc gccaatatca ataaaaagat tttcaaaaat tcctatttgg ctactctcgt
     2401 ccagtggaat taacaaacca cattgaagca tatattgaag tagtcccaca gttttcaagc
     2461 aaacagactt tccgccagca tttggtcctg aaataatcaa aattcgctga ttctcattta
     2521 aaacgatatc taatggaaca atatttttcc cttgcttttg caaagaaagg tataaaagcg
     2581 gatggctggc tttgttccat tgaatgatac atttattttc aacatttgga cgaatagctt
     2641 taatatcaat ggcaaaaaga gcttttgaat gtaggaaatc tatttcacct aaaaaatttt
     2701 gaagctcaag caaatctgga ataaaagggc gaatataatt tgtaatatct gttaaaattc
     2761 taataatttc acgccgttct tctccttcga gttcacgaat gctattgttc aattctatta
     2821 cttcagcagg ttcaatatag actgtttttc cactggctga ttcatcatgt acaatgccat
     2881 tgattttccg tttaaacgct tgtgaaacag gtataaccag cctcccttcg cgtaaagtag
     2941 gcaaagtatc cttttctaca tagccattcg aaaaagcccg ctgtaatatc tcgtttaaag
     3001 ttcgagaaat attattctgt gtagttaaaa tattttttct aattttttct aattgtggtg
     3061 aagcattgtc ttttatatcg ccatattttg taagaatttt atttatctga tcaattagga
     3121 aagaaaagga ttttacagtt ttagacaggc gtgacaattg ggggtaaaaa gtagaattta
     3181 atttagaaaa ataggtcgtt aaaagttgtg cgttttcaag atttgtacga agacaatata
     3241 attcattttc agttaaatat aaaccttcta catgaatttt atgtaatgat ggacgaatat
     3301 cataaatatt gcttaaaggc aattcgttgg tttcagtagt aaggatttga agcatctcat
     3361 tggtttgatc caactgttga attattttgt aaaaatcaga cgaaaaatgt atgctgtcaa
     3421 ttttttcttt tcctaaagtg ctgatacttt tttcttttaa taattgccga atattcccaa
     3481 aatcaatttt ttgctcgaaa ttttgcggat aaaccatctt ttaaattctc atttggattc
     3541 taattttttt gcttcgttcc atatttcgtc catttcctgc aaagtcattt gtttcaaatc
     3601 ttttccttga gaaatagttt tagattccag ataattaaaa cggttgataa attttcgatt
     3661 ggtgcgttcc aacgcatttt ctggattaat gtcgtaaagc cttcccgcat ttattaaact
     3721 aaaaagaaga tccccaaatt cttcttctat tttatctttg tccagtgatt tgatttcttg
     3781 ttgcaattct aaaaattctt cttttacttt ttcccatact tgctcttttt cttcccaatc
     3841 aaaacctaca tatcttgctt tgtcctgaat tctatgagct ttaatcaaag ctggcaatga
     3901 atcaggaaca ccacctaata ctgttttatt accatttttt tcttttagtt tgagtttttc
     3961 ccagttttga actactgtct cggtagaatc ggacttaaca ttagaaaaaa catgaggatg
     4021 tctgtaaata agtttttcat tcaatgcttt acaaacttcg taaatatcaa aatcattatt
     4081 ttcatttgcc atttgagcgt aaaaaataat atgaagcaat aaatctccta attctttttt
     4141 aatttcttcc atatcttttt tcatgatagc atctgacaac tcgtaagttt cttcaatggt
     4201 ttgtaccctt aggctgtcga aagtttgttc tttatcccaa ggacatttct gacgaagttc
     4261 attcattatt tccacaagtc tgccaaattc ttctttaatt tcatcaagcg tacgtttttt
     4321 atcttccata aattatttta aaatgaaata ttaacttaaa gatgacttat ttttattggc
     4381 gtgttatttt ctcaatattt taaaaatatc agccaatgtc gataacaagt tatataagaa
     4441 caaaattata taaattctgt ttacgaagag aattcttttt taaaggaaca aaaaagggaa
     4501 aatatttcaa aaaataattt ccctcgtttt ttaatctttt ctgaaaagtt aaactattat
     4561 ttaccagcgt aatatacctg caattccttc cagttcgttg tataattttt cttttgggac
     4621 attatttacg aattccaatc gtgtagcata tttagcagcg aggtaaaata tttctttttt
     4681 taaaggtatt tcttcaaatg aatcatcata atcttttgct tcttctggtg tggcaaaaat
     4741 tatcccatcg ctgcatcgat atacggaaac atttaagtcc cacggcaaaa tcaaaacctg
     4801 aagttgtccc atttgaaggg cttctaatac attttgtaaa ccagaaacgc cttttggacc
     4861 ttgtgcctca ttaattaatt gcatttcttc ttgttcttca gcttctctca ttataggaga
     4921 gattcgatct aaaatatctt tagctgaagg attttcagga tttgcaggat tagttgcata
     4981 acccacaatc aactgacgaa gaggacgact taagtaattc tcaaataatt ttatttgcca
     5041 atcattgccc atgagaatca atctgtccat ctcaaattca tgaattgatt tttcaagcat
     5101 ttgagctaat gttacataca atttatgaga gaaagtctgt tgtttcgctt ttattttatc
     5161 tttaaatttc attttagcat taaacatttt atcttctaat actcctgatt cgatgatttg
     5221 agaatactca ttgaattctt tccattcatt tggagtaatg gctgcaaaaa catcagttac
     5281 ttccttgact tcattcataa atacttcata aaatctccat ttgttgccat aaatgtgtaa
     5341 cacgcctacc cttctaaact cgtcaaaagc ataaaggata ggagttaaat aaggtttacc
     5401 aaaatgagct tctactctac cacttcgtaa atcaactact ggtaaatcca cctgtaattc
     5461 aaaatgttct actgctaatt ctcctttttc gtttatccac gcaaaaagga ccaaagtacg
     5521 agcttctgtt ctaatttgat ctaaaaaaat tattaattct tcatagaaac tttttccttt
     5581 tttaagctta atattaagct cattcattga ttttaaagta ttttttaatc gatcatacca
     5641 tgctcttccc acattttcag gaatagcagg attaacattg caatatactg aaagcacagg
     5701 ttcatttatg ctgctcacta atgttttaaa tcgttgaatg tcatttttgc ttatcatata
     5761 tctacatttt ttgattgaat tttgttatta ttttatataa attttcatga taatttacaa
     5821 tcggtttaca gaaaaaaata aagtaccaca acccttgcaa tttagcgagc tt
//
LOCUS       DAOSAS010000112         5770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_8827_length_5770_cov_13.542782, whole genome shotgun sequence.
ACCESSION   DAOSAS010000112 DAOSAS010000000
VERSION     DAOSAS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8827_length_5770_cov_13.542782"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(718..>1188)
                     /locus_tag="PLR72_05900"
     CDS             complement(718..>1188)
                     /locus_tag="PLR72_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HOK36767.1"
                     /translation="GITIFLSDSNCQIKDIIHPSGIHPYLDVIMAGPVPPNPAELLMN
                     SRLDELIDELRIQYDYIILDTSPIGIVSDTFLLDRISDINICVARQNYSPREVCHLIN
                     TTYESKKLKNMAVILNGTDNISSYGYGLYKYHYGKKYKYHEETKKTFTSFLKKG"
     assembly_gap    1189..1288
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1289..2721)
                     /locus_tag="PLR72_05905"
     CDS             complement(<1289..2721)
                     /locus_tag="PLR72_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOK36768.1"
                     /translation="MENNNIASSLTENNNETIDLKELIRKHLRKWPWFIVSIVVCFLV
                     ASFYLKIAHPKYQVQATILLRNQNTTPELSNMTLFNNVGLMGISKEVEDEIQVLSSKT
                     LMKQVIDSLEIQTEYYIKNGLRYDEIYPETPIKLIVAENFIDSLKYPLTLQLKRTKKG
                     YSINLKYGKKITKKYELPQLSGSFSTPLGELKLLEIKPLENGESIKIITYPQRMLIAK
                     YSSTITTATVNKNSNVISITTVSASPKKAEVILNKLIELYNLDATFDKNMVLSNSADF
                     IENRLKLISGELLDVELNVENYKKKNNLTDISSEAQLYLETASDYQKKLAEIETQINL
                     ITYVESYLTDASNQFELLPVNIGIQDVALVKLIDDYNLALLERTKILRSSNKNNPVIA
                     QMEQQINGLKQTIISSIKNINNSLSISKNNLLKQESEFISKIKNIPTQERQYLEIKRQ
                     QQIKEELYLFLLQKREENALNLASTIPS"
     gene            complement(2747..3532)
                     /locus_tag="PLR72_05910"
     CDS             complement(2747..3532)
                     /locus_tag="PLR72_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOK36769.1"
                     /translation="MKKNYQIVLISILILFTSCVSQKKIAYFHGLDEQSAETINQSFK
                     TIHEARVCQGDMLSITVSGIDPEAVAPFNLPVVSYASPGTTQIYASPVLQSYLVDVNG
                     YINFPIIGPIKLAGLTKSQAIQHINELLSPYLKNSIVTIQFLNYKVTVLGEVLRPGQY
                     SINNERVTVLDALGLAGDMTIYGKRENVLLIRENFGKLEFVRLNLNATDLFTSPYYYL
                     QQNDILYVEPNVVRNISAQNIPLYLSTLSTIATLVTVIYSISK"
     gene            complement(3630..5492)
                     /locus_tag="PLR72_05915"
     CDS             complement(3630..5492)
                     /locus_tag="PLR72_05915"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOK36770.1"
                     /translation="MDKKEIDTIESSLIEINNTDQTEERKAVPEKSSVLNKSISNNTS
                     TINNKVEIDYSSYTLEELVDALKQCLNQNIEEIKEEVEHIKQHFYKELKSETEEQKNL
                     ENKGVEKEHAIDEKSKIEENFKSLLAEFREKKAHYQANIEKSLNENLAKKQEILEKMK
                     ALSESKDDVSTHINEFKALQQEWRTIGLVPPANLNTLQKNYNAIQEAFWDLIKINNEL
                     KEYDFRKNLEAKVQICETAEKLAKEETDVIIAFQKLQKLHEEWHEIGPVSRELREQIW
                     QRFKNASTIINKKHQSYFESIKKKEQENLIAKIAICEKIEAIDFTNLKSFNDWNEATK
                     NILTLQEEWRTIGFAPKKTNQKIFERYRKACDTFFTAKAEFYKQIRNTYAENYKKKLV
                     LCEQAEALKDSTNWQDTAEKMIQLQKEWKTIGPVSKKYSDDLWKRFISACDYFFEQKK
                     KVNNNQKNSEIENLEKKRDLIARINAFQKTENISVSLLALRALIDEWYMIGHVPFKEK
                     DKIYKEFRLAIEKKYDELNIDFVSRKLEFFKNNLQNIRSKSPNKLYQEREKLMKSNEH
                     LKSEITTYENNLGFFSSSSQRGDKVISELEKKIDALKEEKRLIEEKIKLIEEYL"
BASE COUNT         1819 a          818 c          794 g         2239 t
ORIGIN      
        1 attgaaagaa gaatttattc aattgacgag gttttttgat tgacatcatc cggtgagctg
       61 aaaaataaaa gaagttagag gggcaattgc ttatattgaa gaaaaaaaat tatatgattg
      121 aaaccaatca gcaacaatgt aaaggggagt gccgccatta tcgttgaact aagcgagtta
      181 gaatatcata acattttttc agactcataa aataacatgc gtagataaaa acctttggga
      241 gaaattcgcc aaatgccagc gagtcataaa tctttgtagg attaattgca cttttgctgt
      301 tagaattaag ttgaaaaatc tttatgtagg ataactcatc attttggtta ttggtaactt
      361 tgattcgagt gtattaattc aatatactca ctttctatta taaacaatac taccattgaa
      421 gtgagaaaag ttcaaaaaca ggattcttca ctgatttgtt tagctttctc cttcctcaaa
      481 atattttcaa caaccttatc tatttttaaa atataagtta ttgatattac atccgttata
      541 ttttttatag ttttgtaatt atttattcgc acagctataa aaaaatgtta tttcccctat
      601 ttaggacact actaaaattt tttaaatgaa taaccccgag gcaagcctcg aggaattctt
      661 ttgattaaat aatttagact catctaccaa atccatgaaa tcacttatta ttatttttta
      721 tcctttttta agaaaagaag taaatgtttt tttggtttct tcgtgatatt tatatttttt
      781 tccataatga tatttataca ggccatatcc ataacttgaa atattatctg taccattcaa
      841 aattacagcc atatttttta attttttact ttcgtaagtg gtattgatca aatggcatac
      901 ttctcttgga gaataatttt gacgagctac gcatatattt atatcactga ttctatctaa
      961 tagaaatgta tcagatacga ttccaatagg agaagtatcc agaataatat aatcatattg
     1021 tattcgtaac tcatcgatga gttcatctaa tcttgaattc ataagtaatt cagcaggatt
     1081 aggtggaacg ggacctgcca tgataacgtc cagataagga tgaattcccg aaggatgaat
     1141 aatatctttt atttgacaat ttgaatcaga taaaaatata gtaataccnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga aggtatggtg cttgcaagat tcaaagcatt
     1321 ttcttcgcgt ttctgtaata aaaataaata aagctcttct ttaatttgtt gttgacgttt
     1381 tatttcaaga tattggcgtt cttgcgtggg tatgttttta attttagaaa taaattccga
     1441 ttcctgcttt aaaagattat ttttagaaat acttaaacta ttattaatat ttttaatgct
     1501 gctaataatg gtttgtttta acccattaat ttgttgttcc atttgagcaa ttactggatt
     1561 gtttttattg gaacttctta aaattttagt tcgttcgagt aatgctaaat tataatcatc
     1621 gattaatttt acaagagcta catcttgaat accaatgttt accggtaata attcaaattg
     1681 attactagcg tcagttaaat aactttctac ataagttatc aagttgattt gagtttcaat
     1741 ttctgctaat tttttttgat aatcgctggc tgtttccaga tacaattgag cttcagaaga
     1801 aatatcagtt aaattatttt tctttttata attttcaaca tttaattcta catcaagcaa
     1861 ttccccacta atgagtttta aacgattttc gataaagtcg gcagaattcg agagcaccat
     1921 atttttgtca aatgtagcat caaggttata aagttcaatt aatttattaa gaattacttc
     1981 tgcctttttt ggagaagctg ataccgtagt aatactaatt acatttgaat ttttattcac
     2041 cgtagcagtg gtaatagtag aagaatattt tgcaatcaac atgcgttgag gataagtaat
     2101 aatttttatt gattctccat tttctaatgg ttttatttct aaaagtttaa gttctcctaa
     2161 aggagttgag aatgaaccgg aaagttgagg tagttcatac ttttttgtaa tttttttacc
     2221 atacttcaag ttgatactat atcctttttt ggttcgtttt aattgaagtg ttaatggata
     2281 ttttaacgaa tctataaaat tttcagcaac aattaattta atgggagttt caggataaat
     2341 ttcatcatat cgcagcccat tttttatgta atattctgtt tgtatttcta acgaatcaat
     2401 tacttgcttc ataagtgttt ttgaggaaag aacttgaatt tcatcttcta cttctttaga
     2461 aattcccatc aaaccaacat tattaaacaa cgtcatgtta gacaactctg gtgtggtatt
     2521 ttgatttctg agtaagatag tagcctggac ttgatatttt ggatgagcaa ttttcaaata
     2581 aaaggaagct actaaaaaac aaactacaat cgaaacaata aaccatggcc attttcgaag
     2641 atgttttcta ataagttctt ttaaatcaat tgtctcatta ttattttcag taagcgatga
     2701 ggcaatatta ttattttcca tattatattt attgaatttt tttattttat ttactaatac
     2761 tataaatcac tgttactaaa gtggcaatag tagaaagagt tgataaatat aaaggaatat
     2821 tttgagctga aatatttctt actacatttg gttccacata aagaatatcg ttttgttgca
     2881 aataataata tggagaagta aacaaatcgg tagcattaag atttaaacgt acgaattcta
     2941 attttccaaa attttctctt ataagcaata cattttctct ttttccataa atggtcatat
     3001 caccagctaa acctaaagca tctaatacag taactctttc attattaata gaatattgtc
     3061 ctggtcgcaa aacttcgccc aaaactgtaa ctttataatt taaaaattga atggtaacaa
     3121 tagaattctt taaataaggt gataaaagtt cgttaatatg ttgaattgct tgtgatttag
     3181 ttaaacctgc taatttaatc ggtcctataa tggggaaatt aatataacca tttacatcta
     3241 ccaaataaga ttgtaacact ggtgaagcat aaatttgtgt ggtccctggt gatgcataag
     3301 atactactgg tagattaaat ggagctactg cctccggatc aatacctgaa actgtaattg
     3361 ataacatatc gccttgacaa actcgtgctt catgaattgt cttaaaagat tggttaatgg
     3421 tttcggcaga ctgttcatca agtccatgaa agtaagcaat ttttttctgt gaaacgcaac
     3481 tggtgaataa gattaaaatg cttatcaata cgatttgata attttttttc atatttataa
     3541 acttctatga atttttactg attatattat aaatttgtaa tggaaaaact aaacttgcac
     3601 aaaattttat aaaaaatttt tccatttttt tataaatatt cttcgattaa tttgattttt
     3661 tcttctatta aacgtttttc ttctttaagt gcatcaatct ttttctctaa ctcagatatt
     3721 actttgtccc ctctttggga agaagaagaa aaaaatccta aattattttc atatgtggta
     3781 atttcagact ttagatgctc gttggatttc attagtttct ctctttcctg atataatttg
     3841 ttaggacttt ttgaacgtat attttgtaga ttatttttaa aaaattccaa ttttcgactt
     3901 acgaagtcaa tattcagctc atcatatttc ttttcaatgg ctaaacgaaa ttctttataa
     3961 attttatctt tttctttaaa agggacatgt ccaatcatat accattcgtc tatcaaagct
     4021 cgtagagcca acaatgatac tgaaatattc tctgtctttt gaaaagcatt aatccttgca
     4081 attaagtccc tttttttctc taaattttct atctcagagt ttttttgatt attattaact
     4141 ttcttttttt gttcaaagaa ataatcgcaa gctgaaataa atcgtttcca taaatcatca
     4201 gaatattttt tagaaactgg gccaatagtt ttccactctt tttggagttg aatcattttt
     4261 tcagcagtat cttgccaatt ggtactatct tttaaagctt ctgcctgttc acataaaacc
     4321 agtttctttt tataattctc agcataagta tttcgtattt gtttgtaaaa ttcagctttt
     4381 gctgtaaaaa aagtatcaca cgcttttcta tatcgttcaa aaattttttg attcgttttc
     4441 ttgggagcaa aacctatggt acgccattct tcctgaagag ttaatatgtt tttagtagct
     4501 tcattccaat cattgaaact ttttaaatta gtgaaatcaa tcgcctcgat tttttcgcaa
     4561 atagctattt ttgcaattaa attttcctgt tcttttttct tgatagactc gaaataggat
     4621 tgatgttttt tattgataat agtagaagca tttttaaaac gttgccaaat ttgttcacgt
     4681 aactcgcgtg aaacaggacc tatttcgtgc cattcttcat gaagtttttg taatttttga
     4741 aaagcaataa tgacgtcggt ttcttccttc gcgagttttt ctgctgtttc acaaatttga
     4801 actttggctt ctaaattttt tctaaaatcg tattctttta gctcgttgtt gattttaatt
     4861 aaatcccaaa atgcttcctg aatagcattg taattttttt gtagagtatt taaatttgca
     4921 ggaggtacca aacctatagt cctccattct tgttgtaaag ctttaaattc attgatatgg
     4981 gtagaaacat catctttgct ttcacttaaa gctttcattt tttcaaggat ttcttgtttc
     5041 tttgccaggt tttcattgag acttttttct atattagctt gataatgagc tttcttttca
     5101 cgaaattcgg caagaagaga tttgaaattt tcttcaattt tacttttctc gtctattgca
     5161 tgttctttct caactccttt attttcaaga tttttttgtt cttcagtttc tgattttaat
     5221 tctttataaa aatgttgctt aatatgttca acttcttcct taatttcttc tatattttgg
     5281 ttaagacatt gtttcaaagc atctactaat tcttctaaag tataagaaga atagtcaatc
     5341 tctaccttat tattaattgt tgaagtatta ttagaaattg atttatttaa aacggaagat
     5401 ttttccggaa ccgcttttct ttcttcagtt tgatctgtat tatttatttc gattagagag
     5461 gattcaatag tatcgatttc ttttttgtcc ataaggtttt gcggatagtt cacattgttg
     5521 agtaggttca tttacaatat catttagaaa tgtcacaatc tacttcaaat ataaataaaa
     5581 tattaacagt ttactgtttt aataattagg acaaaaatac gatatttatt tttaatattc
     5641 ataattttat aaatataaaa ttatcagtag agtcagccag caagtgcgaa attaataaaa
     5701 aatgttttaa aatcttgatt ttaaaacata aatgattgat atttcgccct tcaaaaagca
     5761 agggtaaaaa
//
LOCUS       DAOSAS010000113         5762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_8845_length_5762_cov_13.869459, whole genome shotgun sequence.
ACCESSION   DAOSAS010000113 DAOSAS010000000
VERSION     DAOSAS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8845_length_5762_cov_13.869459"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..913
                     /locus_tag="PLR72_05920"
     CDS             95..913
                     /locus_tag="PLR72_05920"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOK36771.1"
                     /translation="MEQKAILITGASSGIGLATALQLMEKGFRVYAASRHGGEYQKAK
                     TSNGEIVPVKMDVNNEEDIKKVIEQILNENQSLYAVISNAGNGIAGAVEDTSIEEAKY
                     QFETNFFGAFKVIKACLPIFRQQEQGKIIATSSVAAIVPIPFQSFYSASKAALLLLMQ
                     GLYMEVKSFGIECCTILPGDTKTNFTFARKYTKASQLPNSAYSEKMKKAVGKMEKDEQ
                     NGMSPEAIARAIVKEITKKHMKALVIPGFQYKAICWLFNRLPVKFRLWVVGLLY"
     gene            complement(1151..1405)
                     /locus_tag="PLR72_05925"
     CDS             complement(1151..1405)
                     /locus_tag="PLR72_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36772.1"
                     /translation="MCKTLFFEWTYYLYEVTSKWNIEKKRSQKVTGKLLGGITEKDEY
                     IESEKERLCKQNALSSLTVKEYGFFYTNNYRENILLLYYF"
     gene            complement(1749..3218)
                     /locus_tag="PLR72_05930"
     CDS             complement(1749..3218)
                     /locus_tag="PLR72_05930"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOK36773.1"
                     /translation="MLIIGIILVFIVFIAAFFKRWNVPLIVIALGIGIFFGSDVTGLI
                     YFDNATLTKKITDIALMFVLFAGGFETKKEDLKIVMKPALILATVGVLVTALVGALIF
                     IFIFKWDFITSLLLCSIISSTDASAVFSILRSRSVDTMATSITKVESATNDPMAIILT
                     TFVLQLKLGAQFNTSSSILIFLWSLVGGIGFGCLIGYLGKFLFNKLKDLDAGYYHLFL
                     LGLIFLSFGLAQLCKASGMMAVFFSGFILGNTKLPYNTRIASFTDTLSFIANVGIYVL
                     LGLLVFPSKFPDIWLYGVILFLIITFIARPVTVFLATYFSKLSIKENIFLSWSGIRGT
                     VPIILATYPIVAGIDPQHQIFNIVFIAVILSILFQGTTIGKLADILKLNNKKKIDVKH
                     LMELVTLHNIDYELIEIFIDDEIYEGECKISDLNLPSNTTITMINRNNEIIAPSGQTT
                     ILPGDVLFVLVEKNNIEETTNQVFSKFFKKNSSEKILSS"
     gene            complement(3270..5408)
                     /locus_tag="PLR72_05935"
     CDS             complement(3270..5408)
                     /locus_tag="PLR72_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK36774.1"
                     /translation="MKFSPFIIFLMMVFLAIPIHAQGLQEDTIQNDSISQKRTKEAVV
                     YGIASNHFAFPLLVMNKNTLQSATFFTPADAMKKEPGIAMSRDGIWATSVNIRGLSEP
                     RLLFLIDDNRILTTIGMAAALSTVDLNSIEKIEIIKGAGSALFGSGAMGGVVNFITER
                     PAYTSFFQVKGKVGSGLHTVNNLWNNDAKIDLTNENWYLSLKGSYRAAQNIQTPTGKL
                     PNSQFNDVSWGIQGGMSYVSNQELLFNYQHFEAWHVGLLERKTFLDTAMVRYKNVKRN
                     LFSVEYIFSDVSDYLKEVRLKAYTQNFDHDIENIVNPSTVILPSSLNSTWGLKAVSNW
                     RFTEYNKLILGAESWLRNTKTICKKITRGDSVTAVVGEQLTPDAEVFDAGIFGQYRWE
                     FSPHYWVLNAGLRLDYFQISNDTAFQPVYRYTYSNSLPSEQKYVANLPRMILYEAGKH
                     RNFSYAAQVDLTYYPTSIQKFTFSLSNSYRMASIEELFKYIDQQSGIIRVGNPSLKPE
                     KGIFTNLSYALDNHRFFLKTDVFANYLFDLITEKKGTFTYINSNGIIENREAYINTNI
                     NKAILYGFEIDVNWKINSFLSTQGNLSCTFGKDIREDEWLPQIPPLKGILTLNYTQKD
                     LFEISLSAIGAAKQSKVAPEEQPTNGHVIFNANIHSVKISVGNDNYFQLLVGANNILN
                     TSYHDHLAATRGLICLEPGRDIYFKLNYGW"
BASE COUNT         2060 a          920 c          856 g         1926 t
ORIGIN      
        1 ataacgattc ctttctaaaa aattgttgaa atttatctca gacaaatgct ataaaaaaat
       61 aaaactatta tatttgcgtc aaaattaaaa agaaatggaa caaaaggcaa ttttaattac
      121 aggtgcaagc agcggtattg gactggcaac cgctcttcaa ttaatggaaa aaggatttcg
      181 ggtttatgct gcttcacgtc atggcggaga atatcagaag gcgaaaacaa gtaatggcga
      241 aatagtccct gttaaaatgg atgtaaataa tgaagaggat ataaaaaaag ttattgaaca
      301 aattttaaat gaaaatcaat cattatacgc tgtcatttcc aatgctggga atggcattgc
      361 cggcgctgtt gaagacactt ctatcgaaga agccaaatat cagtttgaaa ccaatttttt
      421 tggagctttt aaggtaataa aagcttgctt gcccattttt cgtcaacaag aacagggaaa
      481 aattattgct acttcctccg tggcagcaat tgttcctatt cctttccaat cgttttattc
      541 tgcttcaaaa gctgctctgt tgctattaat gcagggactt tatatggaag taaaatcatt
      601 tggcatcgag tgttgtacca ttttaccagg cgacacgaaa accaatttta cttttgctcg
      661 aaaatatacc aaagcctcac aattgcccaa ttctgcttat tcagaaaaaa tgaaaaaagc
      721 agttggaaaa atggaaaaag atgaacaaaa tgggatgtca ccagaagcaa ttgctcgtgc
      781 tattgtaaaa gaaatcacaa aaaaacacat gaaagctctt gtaattccag gttttcaata
      841 taaagctatt tgctggctat tcaatcggct tcctgtaaaa ttccgcttgt gggtagtggg
      901 gttgctgtat tagtttttta aatgggttta aaagtaggga tagaattgta actcaagaaa
      961 taggctcttc tgttttatca ccacaaaaat ccttttattt acttcaagat tattttgtaa
     1021 tagactggaa gttattattc tactgaaaaa acaaatataa aatcaataat cgactcataa
     1081 ataccttctt tacttttcct gttttttggc atatccatca accaagattt tcgagttcat
     1141 atctggacag tcaaaaataa tacaacagta gaatgttctc ccgataattg ttcgtataga
     1201 aaaatccata ttccttgacc gtcaatgaag atagggcatt ttgcttgcat aacctttcct
     1261 tttccgattc tatatactca tccttttcag taatcccgcc aagaagtttt cctgttactt
     1321 tttgagagcg tttcttctct atattccatt tgctggttac ttcataaaga taatatgtcc
     1381 attcaaaaaa caaagttttg cacatttttg cgaaatttta atgcccacta agggaattat
     1441 gtcataggtg taaagaattt tatagttaat tatgtaaata cgtaatgcgg aattataaaa
     1501 aatacaaaca attatagcaa ctttttcttc tattacgtat aaattgagaa gttaagatgt
     1561 aaattatatt tatgactaca atttggtatt attccttttt caattaaaat attaaaaata
     1621 atattgaaaa attattagat tgatatacac ttgtttatac tagtattgtt tgaagcagat
     1681 gttttatttg attaattgaa agaaattttc aagcataatt tattttacga aaatgttata
     1741 aaaaattttt atgatgataa aattttttct gaagaatttt ttttaaaaaa tttgctaaag
     1801 acttgattgg tagtttcttc tatattattt ttttcaacta atacaaaaag tacatcccct
     1861 ggtaaaatag tggtttgccc tgatggtgca attatttcat tatttctgtt aatcatagtg
     1921 atagtagtat ttgaaggtaa gtttaagtca gaaattttac attccccttc atatatttca
     1981 tcatcaataa aaatttctat taattcatag tcaatattat gaagagtcac aagttccatt
     2041 aaatgtttta cgtcaatttt ctttttattg tttaatttca atatgtctgc taactttcca
     2101 atagtagtcc cttgaaataa gatagataat attactgcaa taaaaactat gttaaatatt
     2161 tgatgttgag ggtcaattcc agctacaatt ggataggtgg ctaaaataat aggaactgtt
     2221 cctctaatgc cactccagct tagaaatata ttttctttta ttgaaagttt tgaaaaataa
     2281 gtagcgagaa aaacagttac aggcctggca ataaaggtaa taattaaaaa taatataaca
     2341 ccatataacc atatatcagg gaacttgctt ggaaatacta acaaccctaa taaaacatat
     2401 attcctacat ttgcgataaa ggatagcgta tcagtgaagg atgcaattct ggtattataa
     2461 ggtagttttg tgtttcctaa aatgaaccca ctaaaaaata cagccatcat tccacttgct
     2521 ttacagagtt gtgctaatcc aaaacttaaa aaaattagtc ctaataaaaa caaatgataa
     2581 tatcccgcat ctaaatcttt taatttatta aaaagaaatt tacccaaata tcctattagg
     2641 catccaaaac ctatacctcc cactaatgac cataaaaata ttaagataga agacgaagta
     2701 ttaaattgtg ctcccaattt taattggagt acaaaagtag taagtattat ggccattgga
     2761 tcgttagttg ccgattctac tttggttata gaagtagcca ttgtatcaac agagcgtgaa
     2821 cgcaaaatgg aaaatacagc agaggcatct gttgaagaaa taatggaaca caataataaa
     2881 gatgttataa aatcccattt aaaaatgaat ataaaaatta atgctccaac aagtgctgtt
     2941 actaatactc caacagtggc taagataaga gctggcttca ttacgatttt taaatcttct
     3001 tttttcgttt cgaagccacc agcaaataag acaaacatta aagcaatgtc tgtaattttt
     3061 tttgttaaag tggcattatc aaaataaatt aaacccgtca catcgctgcc gaaaaatatt
     3121 cctattccta aggcaattac aatcaatgga acattccatc ttttaaaaaa agcagctata
     3181 aatacaataa atactaatat aataccgata atgagcaaaa taaattaatt ttaaagtgaa
     3241 tgaaagttaa taatttaaag aaaaaatatt taccaaccat aattgagttt gaaataaata
     3301 tctcttcctg gttctaagca gataagacct cttgtcgcag caagatgatc atgataggaa
     3361 gtattcaaaa tattatttgc acccactaat aattggaaat aattatcatt ccctacactg
     3421 atttttacag aatgaatgtt ggcattaaaa attacatgtc cattagttgg ttgttcttct
     3481 ggagcaactt ttgattgttt ggctgctcct atcgccgata atgaaatttc aaacaagtct
     3541 ttttgtgtat aatttaaagt aagaatacct ttcaaaggag gaatttgtgg taaccattca
     3601 tcttccctaa tatctttccc aaacgtacag gataaatttc cctgtgtaga aaggaaagaa
     3661 ttaattttcc aatttacatc tatttcaaat ccatataata tagctttatt tatattagta
     3721 ttgatataag cttcacgatt ttcaatgata ccatttgaat ttatataagt aaatgttccc
     3781 ttcttttcag taatcaaatc aaataaatag ttggcaaaaa catctgtttt caggaaaaaa
     3841 cgatgattat ctaacgcata acttaaattg gtaaaaattc ctttttcagg tttcaaggag
     3901 ggatttccca ctcttattat tcctgattgt tgatcaatat atttgaataa ttcttctata
     3961 gaagccattc tataagaatt tgacaaagaa aaagtaaatt tttgtatgga agtaggataa
     4021 taagttaaat caacttgagc agcataagaa aaattacgat gtttccccgc ttcatataaa
     4081 atcattcgag gaagatttgc aacatatttt tgttcagatg gcaaactgtt cgaataggtg
     4141 tatctataaa cgggttgaaa ggcagtgtca ttagaaattt ggaaataatc cagccttaat
     4201 cccgcattca aaacccaata atggggagaa aattcccacc gatattgtcc aaaaattcca
     4261 gcatcaaaca cttcagcatc aggagttagc tgctcgccaa caacagcggt aacagagtct
     4321 cctcttgtta tttttttgca aatagtcttg gtattacgaa gccagctttc agctccaagt
     4381 attaatttat tatattcggt aaatcgccaa ttggaaacag cttttaaacc ccacgttgaa
     4441 tttaggctcg aaggtagaat aacagttgat ggatttacaa tattttcaat gtcgtgatca
     4501 aaattttggg tataagcttt cagacgaact tctttaagat agtcgctcac atcagaaaat
     4561 atatattcaa cactgaataa atttcttttt acatttttgt aacgtaccat agctgtatca
     4621 agaaatgttt ttctttccag tagcccaaca tgccacgctt caaaatgctg ataattaaat
     4681 aataactcct gatttgatac ataactcatt ccaccttgaa taccccatga aacatcatta
     4741 aactgactat taggtaattt tcctgttggc gtttgaatat tttgtgcagc acgataactt
     4801 cctttcaatg ataaatacca attttcatta gtaaggtcta tttttgcatc attgttccat
     4861 aaattattga cggtatgtaa acctgatcca actttacctt ttacttgaaa aaaggaagta
     4921 taagcaggac gttcggtaat aaagtttaca actcctccca tagctcctga accaaataac
     4981 gctgaaccag cgcctttaat aatttcaatt ttttcaatgc tgtttaaatc aacagttgat
     5041 aaagctgctg ccataccaat agttgtaaga atcctattat catcaattag aaacaacaaa
     5101 cgaggttctg ataatcctct aatatttact gaggttgccc atattccatc acgcgacatt
     5161 gcaattcctg gctccttttt catagcatct gctggcgtaa aaaaagtagc cgattgaaga
     5221 gtatttttat tcattactaa caatggaaaa gcaaaatggt tagaagcaat gccataaacg
     5281 accgcttctt tggttctttt ttgtgaaata gagtcatttt gaatggtatc ttcctgcaat
     5341 ccctgagcat ggataggaat agcaagaaaa accatcatta gaaaaataat aaatggtgaa
     5401 aatttcatgt gaatttattt ttcaaaaaat tcaattgtat tttttcaaaa ttaaaagaaa
     5461 tattgggttc ccccaaacta atttttaata aaaatgaaag aaattttatg tcggttcaat
     5521 tagtccctct aatttttccg gcaagttccc agaaaaaaga gcttttccaa caaatggaag
     5581 aaacaaattt atttgtttct taagagctgt aaaaaaatac ctgcaaaaat agatttattg
     5641 tgaattaaca atgagagaaa tagggtaaat atctggaaaa aatattcttt atgattcaat
     5701 ataattcata cctaaccgtt atcatatata attaataata ttttttgtat ctttacaaga
     5761 aa
//
LOCUS       DAOSAS010000114         5668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_9008_length_5668_cov_16.679850, whole genome shotgun sequence.
ACCESSION   DAOSAS010000114 DAOSAS010000000
VERSION     DAOSAS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9008_length_5668_cov_16.679850"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /gene="mutY"
                     /locus_tag="PLR72_05940"
     CDS             <1..954
                     /gene="mutY"
                     /locus_tag="PLR72_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOK36775.1"
                     /translation="EIILQQTRVNQGLDYYLRFVERFPNVEMLAAAEEDEVLKYWQGL
                     GYYSRARNLHQAARQIIQDFGGVFPKTYPEVLRLKGVGTYTAAAICSFAYNLPYAVVD
                     GNVYRVLSRLFAIETPIDSHSGKKEFDSLAQQLIENSAPSLHNQALMEFGALQCVPVS
                     PDCSVCPLRVFCKAYELKMVERLPVKSKKTPTKQRFFNYLVINFNQYIFLQKRTADDI
                     WKNLYEFPLIETDHLLTEAELFENDFFQKLFQSIKEISILQISKPVHHLLTHRRITAQ
                     FITLQIAKKSEALKQFIEIPNTEIDDFAVSRLMELYLKNEF"
     gene            1285..2334
                     /gene="mltG"
                     /locus_tag="PLR72_05945"
     CDS             1285..2334
                     /gene="mltG"
                     /locus_tag="PLR72_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOK36776.1"
                     /translation="MSDSFFTKKTLKKIFKWTTIFISIILVYFLYILFFPNIFPPKKT
                     RTFLYIRENSQLKEVVDSLSKNTKVFDVYSFYVASKLLDYESHIKSGRYELQRGMNNF
                     ELIRKLRSGRQTPVLLIINNIRTKEDLAHRLSMQLMVDSASIIQLLNDSTFLAEYGLN
                     PYNSISIFIPNTYEVFWNISPEKLFARMYNEYNRFWNEERRKKAAAIPLTPLEVITLA
                     SIVEEETNVESERPIVAGVYINRLKKGMPLQADPTLKFAAGNFALRRISGIYLKIDSP
                     YNTYKYKGLPPGPIRLPSQKSIDAVLNYTPHNYLYMCAKETLNGEHNFASNWKEHLQN
                     AKKYQQALNRMGIFE"
     gene            complement(2341..3360)
                     /gene="murB"
                     /locus_tag="PLR72_05950"
     CDS             complement(2341..3360)
                     /gene="murB"
                     /locus_tag="PLR72_05950"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013265773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOK36777.1"
                     /translation="MIVYHNFSLLPYNTFQLDIKADSFIEFDSVEELKNILVSPLLLS
                     SPNIMIIGRGSNLLFLDDFKGTILHSGIKFIKKIDENNDFVFLEVGSGIIWDDFVQFC
                     VENDYQGIENLSGIPGEVGAAAVQNIGAYGVEIQGMIEKVNVVEIHSAKIHEFSNADC
                     QYSYRKSIFKKELKGKLVVTSVIFRLNKKSAYKLDYEGLEKEVHAKGEINLKNIRQAI
                     LDIRSRKLPDYKEFGNAGSFFVNPIISKENFQKLQKNYPNIPHYTISEKEEKIPAAWL
                     IEQCGWKGKQIGQVKIHEHQPLILINCGNASGKEIAKVANQIQASVKDKFQIELQPEV
                     NYIYS"
     gene            complement(3344..4270)
                     /gene="miaA"
                     /locus_tag="PLR72_05955"
     CDS             complement(3344..4270)
                     /gene="miaA"
                     /locus_tag="PLR72_05955"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOK36778.1"
                     /translation="MNQSPTLLVLLGPTGVGKTEISLRLAQHFKSPIISADSRQIYKE
                     LKIGTASPSEEQLSTIKHYFIASHTIFENYSAGQFEQEVLLLLNELFQTHQIVLMVGG
                     SMLYIDAVCKGLDNIPSIDAEIRNFWQNKFKDQGLEYLQQELKKLDPVYYQQVDLHNY
                     KRILHALEICSITGKPFSELRTGKAQKRPFHILKIGLTRPRAELYERINKRVDQMIKN
                     GLLEEAMQYYPYRNLNPLNTVGYKEIYDYLDGKYNLEKAIEKIKQNTRHYAKRQLTWF
                     KHDEDIQWFHPEEEEKIIDYVTKNLPLNDCLS"
     gene            complement(4353..4910)
                     /locus_tag="PLR72_05960"
     CDS             complement(4353..4910)
                     /locus_tag="PLR72_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36779.1"
                     /translation="MVFRFTILSDEADDFIRVIDMDAEATFLDLHNAILDSVNFKKNE
                     ITSFFLCSDEWEKEQEITLIEMETSSEYDNLVMDKVKLGDLLTDEKQKLLYVFDMISD
                     RSFFIELSEMIPHKKLSKPVCRISKGNPPEQMLMENDLSTIDKINLDENFYGDESFDI
                     EELDSEGFEDLDFGEGNFSEDDLNY"
     gene            complement(5378..5449)
                     /locus_tag="PLR72_05965"
     tRNA            complement(5378..5449)
                     /locus_tag="PLR72_05965"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5415..5417),aa:Met,seq:cat)
BASE COUNT         1873 a          927 c          872 g         1996 t
ORIGIN      
        1 gaaatcattt tgcagcaaac gcgtgtcaat caagggttgg attattattt gcggtttgtc
       61 gaacgtttcc cgaatgttga aatgcttgct gccgctgaag aagacgaggt tttgaaatac
      121 tggcaaggtt tgggttatta ttcgcgtgcc cgaaatttgc atcaggcagc acgacaaatt
      181 atacaggatt ttggcggtgt ttttccaaaa acttatccgg aagttttacg cttgaaaggt
      241 gttggaactt atacggcagc ggctatttgt tcttttgcct acaatcttcc ttatgccgtt
      301 gtagatggaa acgtttatcg cgttctttca cgcctttttg ctatcgaaac gcctatcgac
      361 agccattcag gtaaaaagga atttgattct cttgctcagc aattgataga aaattctgca
      421 ccttcacttc ataatcaagc tctgatggaa tttggtgctt tacagtgcgt gcccgtttct
      481 cccgattgtt cggtttgtcc tttgcgggtt ttttgcaaag cttatgaact gaaaatggta
      541 gaacggttgc cggtaaaatc caaaaaaaca cccaccaaac aacgattttt taattatttg
      601 gttatcaatt ttaatcaata tatttttttg cagaaaagaa cagcggacga tatttggaaa
      661 aatttgtacg aatttccatt aattgaaact gaccatttat tgactgaagc agaattgttt
      721 gaaaatgatt tttttcaaaa attatttcaa agcatcaaag aaataagcat tttgcaaatt
      781 tcaaaaccgg ttcatcacct tttgacgcat cggcgtatta cggctcagtt cattacttta
      841 cagattgcga aaaagagtga ggcattaaaa caatttatcg aaattccaaa cacggaaatt
      901 gacgattttg ccgtttcgcg gttaatggaa ctgtatttga aaaatgagtt ttaaatttaa
      961 cctgaattaa tctctttaac gtaaaatatc tccccatgtc ctctgtctcg aaaaatttgc
     1021 actcctttgt tgttttaatt gtaagaattc cccgatgctt gcgtcggggt agcttttgtt
     1081 tttatttttt tagaaattcc ggtggcgaag ccataaaatt ggcgtaatac ctcgctgctt
     1141 gcggcgagga tagtcaattt taaggaattt ctttaatagc agatttaaaa tctgtataag
     1201 attctaactt ttaaaaagat tcacgatatt gatttttaaa atatctgaac aaaacagtat
     1261 ttttgtactc ttaattttat ttttatgagt gattcctttt tcacgaagaa aactttaaag
     1321 aaaatattca aatggactac catttttatt tcaataattt tagtctattt tctgtatatt
     1381 ttatttttcc ccaatatttt ccctccgaaa aaaacaagaa cttttcttta tattcgcgaa
     1441 aatagtcaac taaaagaagt ggttgattca ttgagcaaaa acacaaaagt atttgatgta
     1501 tattcttttt atgtcgcctc gaaactgctt gattatgaat cgcatataaa atctgggcgt
     1561 tatgaactac aacgtggaat gaacaatttt gaattaatca gaaaattacg aagcggaagg
     1621 caaactcctg ttttattaat tatcaacaat attcgcacga aagaagactt agcacacaga
     1681 ttatcaatgc aattaatggt agatagtgca agtattattc aattgttgaa tgattcaact
     1741 ttccttgctg agtatggatt aaatccatat aattcaattt caatatttat tccaaatacc
     1801 tatgaagttt tctggaacat aagtccagaa aaattatttg ctcgaatgta taacgaatat
     1861 aacaggttct ggaatgaaga acgtagaaaa aaggcggcag ccataccact tactccattg
     1921 gaagtaatta cgttggcttc tattgtagaa gaggaaacga atgtagaatc cgaacgaccg
     1981 atagttgctg gtgtttatat caatcgttta aaaaagggaa tgcctttgca agctgaccct
     2041 acactcaaat ttgctgctgg aaattttgct ttgcgaagaa tcagcggaat ttatttaaaa
     2101 atagattcac cttacaatac atacaaatac aaaggtttgc ctcctggacc tattcgatta
     2161 ccctcacaaa aaagtattga tgcggtttta aattatacgc ctcacaatta tctttatatg
     2221 tgtgctaaag agactttaaa tggtgaacat aattttgctt caaactggaa agagcacttg
     2281 caaaatgcca aaaagtatca gcaggcttta aatagaatgg gaatttttga atagaaaata
     2341 tcaggaataa atataattca cctcaggttg taactctatt tgaaatttat ctttaacaga
     2401 agcttgaatt tggttagcta ctttggcaat ttcttttcct gaagcatttc cgcaattaat
     2461 taaaattaga ggttgatgtt cgtgtatttt tacctgtcca atttgttttc ctttccaacc
     2521 acattgttca attagccagg cagcaggtat tttttcttct ttttcagaaa tagtataatg
     2581 aggaatgtta ggatagtttt tctgtaattt ctggaagttt tcttttgaaa taattggatt
     2641 gacaaaaaaa ctgccagcgt tcccaaattc tttgtaatca ggtaatttac gtgaacgaat
     2701 atctaaaatg gcttgtctaa tatttttcaa attaatttct ccttttgcat ggacttcttt
     2761 ttcgagacct tcgtagtcta atttgtaagc tgatttttta ttcaagcgaa aaatcacaga
     2821 agtgactacc aattttcctt tcaactcttt tttaaaaata ctttttcgat aactgtattg
     2881 acaatcggca ttggaaaatt catgaatttt tgcagagtga atttctacaa catttacttt
     2941 ttcaatcatt ccttgaattt ccacaccata agcaccaata ttttgtacgg cggctgcacc
     3001 cacttcacca ggaatgcccg aaagattttc aattccttga taatcgtttt caacacaaaa
     3061 ttgtacgaaa tcgtcccaaa taattcctga acctacttct aaaaagacaa aatcattgtt
     3121 ttcgtcaatt tttttaatga attttattcc agagtgtaaa attgtacctt taaaatcgtc
     3181 taaaaacaac aaattacttc ctcttccaat aatcattata ttaggcgaag ataaaagaag
     3241 tggtgacaca agaatatttt ttaactcttc aacagaatcg aattctatga acgaatctgc
     3301 ttttatgtca agctggaaag tattgtaggg caaaagagaa aagttatgat aaacaatcat
     3361 ttagtggcaa gtttttagtt acataatcaa ttattttttc ttcttcctct ggatgaaacc
     3421 attggatgtc ttcgtcatgc ttaaaccatg tcaattgtcg cttcgcatag tgacgtgtgt
     3481 tttgcttgat tttttctatg gctttttcca gattgtattt tccatccaaa taatcgtaaa
     3541 tttctttata tcctactgta ttcaagggat ttagatttct gtaaggataa tattgcatag
     3601 cttcttccaa caaaccattt tttatcattt gatccactct tttattgata cgttcgtaca
     3661 attctgctcg tggacgtgtt agaccaattt tgagaatatg aaacggtctt ttttgagctt
     3721 taccagttcg aagttcagaa aaaggttttc cagttataga acatatttcc aatgcatgta
     3781 aaatgcgttt gtaattatgt aaatctactt gctgataata aacaggatct aatttcttta
     3841 attcttgctg gagatattca agtccttgat ctttaaattt gttttgccaa aaatttcgta
     3901 tttctgcatc aatagaagga atattatcca gccctttaca tacggcatca atataaagca
     3961 ttgatccacc aaccattaag acaatctgat gggtttgaaa tagctcattt aataataaaa
     4021 gtacttcttg ttcaaattgt ccggcgctat aattttcaaa aatcgtgtga gaagcaataa
     4081 aataatgttt tatagttgag agttgttctt ctgaaggaga agcagtaccg attttcagtt
     4141 ccttataaat ttggcgtgaa tcagcagaaa taatgggact tttaaaatgt tgagccaatc
     4201 gaagacttat ttcagttttc ccaactcctg ttggtcctaa caaaactaat aaagtgggtg
     4261 attggttcat ttgaaaaggg atattaactc aaataaaaaa acagaaaata aaaaaacaat
     4321 tattttctgt tcccaataga taattctaag tattaataat ttaagtcgtc ttcagaaaaa
     4381 ttaccttctc caaaatcgag atcttcaaat ccttcactgt ccagttcctc tatatcaaat
     4441 gattcatcgc cataaaaatt ctcatctaag tttattttat caatagtaga aagatcattt
     4501 tccatgagca tttgttcggg cggatttcct tttgaaattc tgcaaacagg ttttgataat
     4561 tttttatgag gaatcatttc acttaattca ataaagaaac ttctgtcaga aatcatatca
     4621 aagacataaa gtagtttttg cttttcgtcg gtcagcaaat ctcctaattt tactttatcc
     4681 attactaagt tatcatattc tgaagaagtc tccatctcaa tcaaggtaat ttcctgctct
     4741 ttttcccatt catctgagca cagaaagaaa gaagtgattt catttttttt gaaatttact
     4801 gaatcaagta tggcattatg cagatccaga aaagttgctt ctgcatccat atcaattact
     4861 ctgataaaat catctgcttc gtcagataaa attgtaaatc gaaaaaccat attattatta
     4921 aagattataa ttacgcctgt aaaattaata ttttttttga gagtatgaaa tagctgtaat
     4981 tttatcctaa gaccaacgta taaaaaattt caaaaatgaa taattattaa tagtctaata
     5041 aattgattat taataattta tattgtaaat tacaaaaaaa ttatctgcct ttaaaagcaa
     5101 aaaagatatg acaaagcaaa aaacatcttt acacgagtat tttaaaattt tcctgattat
     5161 tgtgttaaca agaataaaaa gcataagctt tctgacatta tttgctaata atcaaatggc
     5221 tacattctaa atgaaattca ctcaaatttt ctgaaaaatc acaatttttt tctatctaaa
     5281 atagaaaaag atcgtatttc tcccttaagg aaaaagaaaa ttaagcaaag atcaaataaa
     5341 aaagaagaaa cattttgcaa tatttcttct ttaaaagttg cggaggctcg attcgaacga
     5401 acgacctttg ggttatgagc ccaacgagct accactgctc cactccgcga ttttattttg
     5461 caaagataat attttatttt gtaaataaca atagaatatt ctttcttcga caaaaataat
     5521 cactttgaaa cgttataaat gtttttaaat tttttgctgt cctaaatcat tgtgatttta
     5581 catcttcaca gattattttg aaatacaata gagaactaaa ttattttaaa tgttattttt
     5641 ttactacttc aaaatatagt atatttgc
//
LOCUS       DAOSAS010000115         5497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_9361_length_5497_cov_12.701580, whole genome shotgun sequence.
ACCESSION   DAOSAS010000115 DAOSAS010000000
VERSION     DAOSAS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9361_length_5497_cov_12.701580"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1461
                     /locus_tag="PLR72_05970"
     CDS             169..1461
                     /locus_tag="PLR72_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOK36780.1"
                     /translation="MNFKPIIFIGIFLLLFFWQSKTQNFDDLFTESPDILQHTDTNAN
                     DTEITIPFGFISNLDQLLHSWALQRIDTSCSANEEIPIVSDSVYKVRLSQMPCLIEMP
                     FHPVVRSFIELYTIQKRTLMEYMLGMSNYYFPIFEQILDANKLPLELKYLPIIESALN
                     PIAISRMGAAGLWQFMISTGKMYGLEINTLIDERLDPVKSTKAAARFLKDLYDIYGDW
                     NLVIAAYNCGPGNVNKAIRRAGGKTDYWTIYPYLPAETRGYLPIFIAATYSFHYAAEH
                     NLCPAIIDIPIITDTFTVHQRIHFEQIASVLHIPIEELRWLNPQYRKDIVPGDIKPYT
                     ITLPLNYSSAFIDKFDEITAYNADELINNRRNEVTIMKASSPSLGGSGKVTYHKVRKG
                     ETLGGIAAKYGVSVSQLKKWNNIKGTLIKPGQQLKIIK"
     gene            1475..1795
                     /locus_tag="PLR72_05975"
     CDS             1475..1795
                     /locus_tag="PLR72_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOK36781.1"
                     /translation="MEKLYYSISEVAEMLDVTPSNLRFWEKEFTQLRPKRNEKGTRFY
                     SQKDIQTIKQIKYLVDEQKLTLEGARLKLSQKKDEVAKQQELLERLKGIRNELLKIIQ
                     ALND"
     gene            1808..2926
                     /locus_tag="PLR72_05980"
     CDS             1808..2926
                     /locus_tag="PLR72_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOK36782.1"
                     /translation="MKKNISIGIMIFIFLVINSSAFGQQRPKNIIIMIGDGMGLPQIY
                     TAMVANNNHLALEDCTYSGFSKTYSADHFTTDSGAGGTAIACGIKTKNGMIGMGPDSV
                     KVTSILEISEQNQLSTGIVVACAVTHATPASFIAHQVDRDMYEEIAADYLKTDIDVFI
                     GGGRQYFEKRKDKRNLLNELKNKNYQLASTLEEVKKIKSGKLAGLLYEDHPSSIPERG
                     NMLPEATMAAIDILDNNPKGFFLMVEGSQIDWAAHDNNTKQIIKEMLDFDQTIQQVLN
                     YAKEKGNTLVIITADHETGGMTLLDGKMGSDSLKAEFSTKNHSGIPVPVFAFGPGAEN
                     FTGFMENTDFKPKIEKLLDLKDNDSAIKIKNNIHKKNQ"
     gene            2945..4052
                     /gene="prfB"
                     /locus_tag="PLR72_05985"
     CDS             join(2945..3007,3009..4052)
                     /gene="prfB"
                     /locus_tag="PLR72_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445733.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOK36783.1"
                     /translation="MVTIEQLKDLSERQEALRRYLDIENKQIQLQEEELRTQVPDFWD
                     DPKTAENQLRKVKELKNWLTDYNEVKDAVDELKLAFDFFNEEAISEKELDETYQRALN
                     LVENLEMKNMLSHEEDQLGAVIKIVAGAGGTEAQDWAEMLLRMYQRWCERKGYKWEIA
                     SFQPGEEAGMKSVTLQVEGDKAYGYLKSENGVHRLVRVSPYNAQGKRMTSFASVFVVP
                     LIDDSIEIEINPANLTWDTFRSSGAGGQNVNKVETGVRLHYTFKHPLTGEMSEIVIEN
                     TESRSQFDNKENAIRLLKSQLYELELERRRAEQAKLEANKKKIEWGSQIRSYVFDDRR
                     VKDHRTNYQTSNVQAVMDGDIDDFIKAYLMEFSD"
     gene            4219..5190
                     /gene="argC"
                     /locus_tag="PLR72_05990"
     CDS             4219..5190
                     /gene="argC"
                     /locus_tag="PLR72_05990"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOK36784.1"
                     /translation="MKKIKVGIVGGAGYTAGELIRILIYHPEVEIIFIHSKSKAGKQV
                     AEIHSGLIGETDLQFTSELPFEKIEVLFLCLAHGESKQFIETNKIPTEIKLIDFSADF
                     RLDMHSNFVYGLPELQAEKIKKAIHIANPGCFATAIQLALLPLAKNGLITNEIHVSGI
                     TGSTGAGVKPSETSHFSWRNNNVSVYKAFTHQHLQEIQQTLHQLQPSFNQEINFIPMR
                     GNFTRGILVSCYTHCSLNENDAKALYKEFYEKSAFTFVTDKNPDLKQVVNTNKALVYL
                     EKYNEKLFIVCVIDNLLKGASGQAVQNMNLMCGLDEKTGLQLKAIGF"
BASE COUNT         1960 a          839 c          995 g         1703 t
ORIGIN      
        1 tggtagatgc ttttgtagat gctcaattat atgattttga cattacccct gagttatcaa
       61 tgggtttcgg accaactctt ttgattgata attcggaggc acaaagaata ttaggaatgc
      121 aactgtgttt ccatttgaaa tgaattataa aaaatagttt ccccaaaaat gaactttaaa
      181 ccaataattt tcataggtat ttttttattg ctgttttttt ggcaatccaa aacacaaaat
      241 tttgatgact tattcactga gagtccagat atcttacaac atactgatac caatgcgaat
      301 gatacagaga ttactatacc atttggattt atttcaaatt tagatcaatt gttacattca
      361 tgggctttac agagaattga tacaagttgt tcagccaatg aagaaattcc tattgttagt
      421 gattctgttt ataaagtacg tttatcgcaa atgccttgtt taatagagat gccttttcat
      481 cctgtggtac gatcattcat agaattatat actattcaaa agcgtacttt aatggaatat
      541 atgttgggaa tgagtaatta ttatttcccc atttttgagc aaattttgga tgctaataaa
      601 ttacctttag aattaaaata tcttcctatt attgaatctg cattgaatcc aattgcaata
      661 tctcgaatgg gagcggctgg attgtggcaa tttatgattt ctaccggtaa aatgtatggt
      721 ttagaaataa atacgttgat tgacgaacgc ttagaccctg tgaaatctac caaagctgca
      781 gcacgttttt taaaggattt atatgatatt tatggagact ggaatcttgt aattgctgct
      841 tataattgtg gaccaggtaa tgtaaacaaa gccattcgaa gagctggagg taaaacagat
      901 tactggacaa tttatcctta tttgcctgca gaaacaaggg gatatttacc tatttttatt
      961 gctgccactt actcattcca ttatgctgct gaacacaact tatgtcctgc tattatagat
     1021 attccgatca taacagatac ttttacagtt catcaacgaa ttcattttga acaaattgct
     1081 tctgttttgc atattcctat tgaagaactt cgctggctga atccacaata tcgtaaagac
     1141 atagtacctg gtgatattaa accttacact attaccttgc cattaaatta ttccagtgct
     1201 tttatagata aatttgatga aattacagct tataatgccg acgagttaat taataacaga
     1261 agaaatgaag taactattat gaaggcttct tcaccatcgc tgggtggttc ggggaaagtt
     1321 acttatcata aagtaagaaa aggtgaaacc ttaggaggaa ttgcagctaa atatggagta
     1381 tcagtttccc aattaaaaaa atggaataac attaaaggaa ctctaataaa gccgggacaa
     1441 caactgaaaa ttatcaaata aagtaaccca taatatggaa aaactttatt attcaatatc
     1501 tgaagtagcc gaaatgttag acgtaacgcc ttccaattta cgcttctggg aaaaagaatt
     1561 cactcaactt cgaccaaaac ggaatgaaaa gggaacacgc ttttattccc aaaaagatat
     1621 ccagaccatt aagcaaatca aatatttagt tgatgagcaa aaattaacac tggaaggggc
     1681 aagattaaaa ttatctcaaa aaaaagatga agtagcaaaa caacaggaac tccttgaacg
     1741 tttaaaaggc atacgaaacg aattattaaa aattatacaa gctttaaacg attaatcatt
     1801 ttggaaaatg aaaaaaaata ttagtattgg aataatgata tttatctttt tggtgataaa
     1861 ttcatcggca tttggtcaac agagaccaaa gaatatcatt attatgatcg gtgatggaat
     1921 ggggctacca caaatttata cggctatggt tgccaataat aatcacttgg cactggaaga
     1981 ttgtacttat tctggttttt caaaaactta ttctgccgat cattttacta ccgattctgg
     2041 agcaggagga acagctattg cttgtggtat taaaacaaaa aatggaatga tcgggatggg
     2101 acctgattcc gtaaaagtaa cttctatact cgaaatttct gaacaaaatc aactttcgac
     2161 gggcattgta gtagcttgtg ctgtgaccca cgctacaccg gcgtctttta ttgctcatca
     2221 agttgatcga gatatgtatg aagaaatcgc tgccgattat cttaaaacag acattgacgt
     2281 atttattggt ggtggtcgtc aatatttcga aaaacgcaaa gataaacgca accttttaaa
     2341 tgaactgaaa aataaaaatt atcaacttgc ctctacactg gaagaagtaa aaaaaatcaa
     2401 atcaggcaaa ttggcaggct tgttgtatga agatcatcct tcttccatac ctgaaagagg
     2461 aaatatgctt cctgaagcga caatggctgc cattgacatc ctggataata atccaaaagg
     2521 atttttcctg atggtagaag gttctcagat cgattgggct gctcacgata ataataccaa
     2581 gcaaattatc aaagaaatgc tcgattttga tcaaaccatc cagcaggttt taaattatgc
     2641 aaaagaaaaa ggtaatacat tggtaattat aactgctgat catgaaacag gtggaatgac
     2701 tttacttgac gggaaaatgg gttcagattc attaaaagct gaattcagca caaaaaatca
     2761 ttcaggtatc ccagttcctg tttttgcttt cggccctggt gcagaaaatt ttacaggttt
     2821 tatggaaaat actgatttta aaccaaaaat agaaaaattg ttggatttaa aagataatga
     2881 ttcagcaata aaaataaaaa acaacattca taaaaagaat caataattag gaactaatta
     2941 aattatggtt actattgaac aattaaaaga tttatcagaa cgccaggagg cgttgaggag
     3001 gtatctttga catagaaaac aaacaaattc aacttcaaga agaagaattg cgtacccaag
     3061 tgcctgactt ctgggatgat cctaaaactg cagaaaacca attgcgaaag gtaaaggaat
     3121 taaaaaactg gttgacagac tataatgaag taaaagatgc agtggatgag ttgaaattgg
     3181 cttttgattt tttcaatgaa gaagctatta gtgaaaaaga actcgatgag acttatcagc
     3241 gtgctttgaa ccttgttgaa aatctggaaa tgaaaaatat gctttcgcac gaagaagatc
     3301 aattgggagc tgtgataaaa attgtagctg gagcaggcgg gacagaagct caggattggg
     3361 cagaaatgct tttgcggatg tatcagcgat ggtgtgagcg gaaaggctac aaatgggaaa
     3421 ttgcttcttt ccaacctggc gaagaagcag gaatgaaatc ggtaacttta caagttgaag
     3481 gcgataaggc ttatggttat ttgaaaagtg aaaatggtgt tcatagatta gttcgcgttt
     3541 cgccttataa tgctcaaggt aaacgaatga cttcttttgc ttccgttttt gtggttcctt
     3601 tgattgatga tagcatcgaa attgaaatca atccagccaa tcttacctgg gatacttttc
     3661 gctcttcagg tgctggtggt caaaatgtaa acaaagtaga aacgggagta aggttacact
     3721 acacttttaa acacccactt actggcgaaa tgagcgaaat cgtaattgaa aatacagaaa
     3781 gccgttccca attcgataat aaagaaaacg ccattcgatt gctaaaatca caattatacg
     3841 aacttgaatt ggaacgaaga cgtgccgaac aagcaaaatt agaagcaaac aagaaaaaaa
     3901 ttgaatgggg ctcacaaatc agaagttatg tttttgacga caggagggta aaagaccatc
     3961 gcactaatta tcaaacttcc aatgtgcagg cagtaatgga cggcgatatc gatgatttta
     4021 tcaaagctta tttaatggaa ttctccgatt aattttttgc ttttttcatt ttttctctgc
     4081 aaacaagttt ttccaaaaaa tcattataac tatttgttta aaaacaaatg actcatatta
     4141 actttatctt taaaaataga aaagttcatt ttgttataaa aaagatcgaa aatttattga
     4201 attaaatatc tgaaagttat gaaaaaaatt aaagtaggta ttgtaggagg tgcgggctat
     4261 acagcaggtg aattaattcg tattttaatt tatcatcctg aagttgaaat tatctttatt
     4321 catagcaaaa gtaaggcagg aaaacaagta gctgaaatac acagcggctt gattggtgaa
     4381 actgacctgc aatttaccag cgaattacca tttgaaaaaa tagaagtttt atttttatgc
     4441 ctggcacacg gtgagagtaa acaatttatt gaaactaata aaattccaac tgaaataaaa
     4501 ctgattgatt tctctgctga ttttcgtctt gatatgcatt ctaattttgt gtatggattg
     4561 cctgaacttc aagctgaaaa aataaaaaaa gctattcata tagccaatcc agggtgcttt
     4621 gctactgcta ttcaactggc attgttacct ttggcaaaaa atggtttgat tactaatgaa
     4681 attcacgtat cgggaattac tggctccact ggagctggag taaaaccctc agaaacaagt
     4741 cattttagct ggcgtaataa caatgtttct gtttataaag cttttactca tcaacatttg
     4801 caggaaattc agcaaacgct tcatcaactg caaccttctt tcaatcaaga gattaacttt
     4861 attccaatga gaggcaactt tactcgtgga attttagtct cttgttatac ccattgctca
     4921 ttgaatgaaa atgatgcaaa agctttatac aaagagtttt acgaaaaaag tgcttttact
     4981 tttgtaacag ataaaaaccc agatttgaaa caggttgtaa atactaataa agcacttgtt
     5041 tatttagaaa aatacaacga aaaattattt attgtttgtg tgattgataa tttgttaaaa
     5101 ggtgcttcag ggcaggcagt ccagaatatg aaccttatgt gtggacttga tgaaaaaaca
     5161 ggcttacaat tgaaagcaat cggattttga aggaaaattg taattaaatc agtttaagtt
     5221 atattttttg taagcttttc ggaaaataaa ttgaattgaa ttgaattgtt cttttctaaa
     5281 taattgttaa aactaagagc aaattttatc aaagtaaata atttgaactt ggcaaaaatc
     5341 gctttagata aactctataa atcaattcag agtaaattga tatttcttct gaaatattga
     5401 attaaaatta aaattatttt tagtgagaaa gtttcaccac gttgttgttc tttccctcgt
     5461 gataaagtga aatataatcg aatgaaacaa taaaaaa
//
LOCUS       DAOSAS010000116         5469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_9407_length_5469_cov_10.526598, whole genome shotgun sequence.
ACCESSION   DAOSAS010000116 DAOSAS010000000
VERSION     DAOSAS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9407_length_5469_cov_10.526598"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLR72_05995"
     CDS             <1..611
                     /locus_tag="PLR72_05995"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOK36785.1"
                     /translation="ISFLGTQIGSSLKEQEKIGLMGDSTLKTDINLIKQGLVNGLRGS
                     NIQMEPEEAKVYLQSTMQNRQAKQIEKEYAATKAAGELFLKENAKRPGVIITPSGLQY
                     EIIKKGNGPIPTDSSKVKVHYHGTLIDGTVFDSSVERKEPSVFYVNQVIKGWQEILKL
                     MPVGSKYKIYVPQELAYGGQEQGLIKPYSPLIFEIELLSIEK"
     gene            822..1685
                     /locus_tag="PLR72_06000"
     CDS             822..1685
                     /locus_tag="PLR72_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HOK36786.1"
                     /translation="MDKKTRKLLISVQRNEITEHFIYKRLAEGCKDQKNAEILHTISE
                     QEKQHAAFWQEKTNLKVKPDYWRITYTVFLARLLGLSFVLKQMEKREGTGSKLYNELS
                     NIIPETKFFAEEESIHEQQLLTMLDEERLQYVGSIVLGLNDALVELTGALAGFTLTLG
                     TTRIISLAGLVTGISAALSMAASDYLSSKAEGNKKAKKSAAYTGITYFFTVIFLILPF
                     LLLSSRFFALSLTLLLAVLIIFFFNYYISVAKELEFKSRFFEMCFISLGVAAFSFFIG
                     YILNSVLGVNA"
     gene            complement(1959..3269)
                     /locus_tag="PLR72_06005"
     CDS             complement(1959..3269)
                     /locus_tag="PLR72_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOK36787.1"
                     /translation="MIIDKELVDSLFAQFGTSDINQLSIREIGKLVSQIEKQSGIEFI
                     HMEMGVPGLPTLPVTVQAEKEALDKGLTSLYSDIEGIPFFKTETARFAKLFINLEVSP
                     LNCIPTVGAMQGSFAALLASARTNVKKPYTLFIDPGFPVNKSQLQLLGLPYKSFDILN
                     FRGEKLREKLLSYLKEGDICTITYSSPNNPSWINLTEEELQIIGELATQYDVIVVEDL
                     AYFGMDFRYDYGKPGVPPYQPTVAHYTDNYILLLSASKIFSYAGQRIGMMIISEKLRT
                     RHYPPLKNYFTSDVLGHFIPYGVLYATTAGTSHTAQYAVAATLKAINDGEVDFLKELA
                     VYGEKARQMKSLFLKNGFYLVYDEGGKLADGFYFTVAYPGMTSNELLKEFLYYGLSAI
                     SLTITGSSDPNGLRICVSFVRNDQIPELEKRLADFSKERTRKKN"
     gene            complement(3354..4664)
                     /locus_tag="PLR72_06010"
     CDS             complement(3354..4664)
                     /locus_tag="PLR72_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOK36788.1"
                     /translation="MIWNEEVECMSHEEMRELQSERLRNLVNRVYRNVPFYRKKMEEM
                     NITPSDIHSIDDISKLPFTYKNDLRDNYPFGLFAVPMKDIVRVHASSGTTGKPTTVGY
                     TKNDIQNWCESLARCLTMAGVTKEDVVQVSYGYGLFTGGLGLHYGAETIGATVIPASG
                     GNTKRQLQLMSDYGVTVLACTPSYALHLVDAMSEFGYSRADMKLRLGIFGAEPWTENM
                     RLELERKWGIQAFDIYGLSEVMGPGVAHDCSFHQGLHINEDHFFPEIVDPKTKQPLPD
                     GCEGELVFTTLTKEGIPLLRYNTRDLSMLNHEKCECERTLVRMKKITGRSDDMLIIRG
                     VNLFPSQIEQVLLEMCETSAHYLLCVDRKDNLDYLELKVELDESKMVETIRELESLSK
                     RIEHSLRSAIGLSIKVTLVEPRSIPRSEGKAVRVIDNRKKEESK"
     gene            complement(4661..5089)
                     /locus_tag="PLR72_06015"
     CDS             complement(4661..5089)
                     /locus_tag="PLR72_06015"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOK36789.1"
                     /translation="MKIRQLSVFVENKTGHLNEILNILARNNINIIALTLADSSDYGL
                     LRMLVSDPLKAVEVLRAENCSVRIHDVLSLELEGVPGSLARILDKFTAENISIEYLYA
                     FSFGSKSIMVMRTNNMDKATEVIKKYQFKPIYESDLKLTV"
     gene            complement(5229..>5469)
                     /locus_tag="PLR72_06020"
     CDS             complement(5229..>5469)
                     /locus_tag="PLR72_06020"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HOK36790.1"
                     /translation="GMIVGTPGYCPLTDWHFMVLEKLGKTNLPNSYLKYLFDRITGLN
                     INAELVDKVTLIVFLAALAISIFLNILDKVVLKRK"
BASE COUNT         1848 a          977 c          894 g         1750 t
ORIGIN      
        1 aaatttcttt tttaggtact caaattgggt cttcactaaa agagcaggaa aaaattggct
       61 taatgggtga ctcaacttta aaaactgata tcaacctaat taaacaagga ttggtgaatg
      121 gactaagggg atcaaacatt caaatggaac ctgaagaagc aaaagtatat ttgcagagca
      181 ccatgcagaa tcggcaggct aaacaaattg aaaaagaata tgctgccact aaagctgcag
      241 gagaattatt tttaaaagaa aatgccaaaa gaccgggagt aattatcact ccaagtggct
      301 tacaatacga aattatcaaa aaaggaaatg gtcccatacc gactgatagt agtaaagtaa
      361 aagttcatta tcacggtacg ttgatagatg gaactgtttt tgacagttct gtggagcgta
      421 aagaacctag tgtattctat gtcaatcaag taatcaaagg ttggcaggaa attctaaaat
      481 taatgccagt aggttcgaaa tataaaattt atgttcctca ggaattggct tatggtggtc
      541 aggaacaggg attaattaaa ccctattcac ctcttatttt tgaaattgaa ttactttcaa
      601 ttgaaaaata aaagcagctt tatagcaatt tcaatatctt ataaaaggat ataaaagaaa
      661 ttttaataaa ccctggttga caataaactt tcaacgaggg ttttttgttt tttaaaatct
      721 tgtttttctg cattggaacg gattaatatt tttttacttt gtttttctta gtttatttct
      781 aattaaaatt aattgtctta taaaatcaaa ataaatatac tatggacaaa aaaacgagaa
      841 aactattaat atcagtacaa cgaaatgaaa tcactgaaca ttttatttat aaacgtttag
      901 cagaaggatg taaagatcaa aaaaatgctg aaattttaca caccataagc gaacaagaaa
      961 aacaacatgc agcattttgg caagaaaaaa ccaaccttaa agttaaacca gactattgga
     1021 gaattactta cactgttttt ctggctcgtt tattgggact gtcgtttgta ttaaaacaaa
     1081 tggaaaaacg cgaaggaaca ggttcaaaac tttataatga attgtcaaat attattcctg
     1141 agacaaaatt ttttgctgaa gaagaaagca ttcacgaaca acaattatta acaatgctcg
     1201 atgaagagcg attacaatat gtcggttcta ttgtgttagg tcttaatgat gctctcgtcg
     1261 aattaaccgg agcactggca ggttttactt taactttggg aacaaccaga atcatttcat
     1321 tagcaggatt agttacagga atatctgctg ccttgtctat ggcggcttcc gattatcttt
     1381 cctcgaaagc agaaggtaat aaaaaagcca aaaaatcggc tgcttataca ggtataactt
     1441 attttttcac tgttattttt ctcattcttc catttttatt attatccagc agattttttg
     1501 ctttatcgct aacattatta ctggcagtat taataatttt cttttttaat tattatatct
     1561 ctgtggcaaa agagctcgag tttaaatctc gtttttttga aatgtgcttt atcagcttag
     1621 gagtagcagc cttttcattt tttataggtt atatacttaa tagtgtgtta ggcgtaaatg
     1681 cttaatagaa aaagttttga gtttacgaag ttgcgggaag aatgaagatt tttttgtagg
     1741 gaaattttaa tttgtatcaa aacaactgaa gcaaagctga aattgccaaa acaataaaaa
     1801 aaattgagta aaaatttctt acattcaatt ttgaaaagtc tcaatttttt gaatattttg
     1861 tcgatacatt atattgaaat ttaaataatt ataaagtact ttttctaatg actgccatta
     1921 gaaattcaat cctaatgaca aatttgggat aaattaactc aatttttttt tcttgttctt
     1981 tcttttgaaa aatctgctaa tcgtttttct aattcaggaa tttggtcatt tcttacaaag
     2041 gagacacaaa ttcgcaaacc atttgggtcg ctgcttcctg taatggtcaa cgaaatggca
     2101 cttaaaccat aataaagaaa ttctttcaat aattcgttgc ttgtcattcc agggtaggca
     2161 acagtaaaat aaaaaccatc tgccaatttt ccaccttcat cataaacgag ataaaaacca
     2221 ttttttaaaa acaatgattt catttgtcgg gctttttctc cataaacagc aagttctttt
     2281 aaaaaatcaa cctcgccatc gtttattgct ttcaaggtag cagctaccgc atattgtgca
     2341 gtatgtgaag ttccagcagt tgttgcatac aaaactccat aaggaatgaa atgtcccagc
     2401 acatcactgg taaaataatt tttcaaaggt ggataatgtc gagttcgtaa cttttcagaa
     2461 atgatcatca ttcctattcg ttgaccagca tagctaaaaa ttttcgaagc agaaagtaac
     2521 aaaatataat tatctgtata atgtgccact gtgggttgat aaggcgggac accaggcttt
     2581 ccataatcgt aacgaaaatc cattccaaag taagccagat cttctaccac gatcacatca
     2641 tattgagtag ccagttcacc aatgatttgc aattcttctt ctgtcaagtt aatccatgag
     2701 ggattattag gagacgaata agtaattgta caaatatccc cttcttttaa ataggaaagc
     2761 aatttctctc gaagtttctc acctctgaaa tttaaaatat caaacgattt ataaggtaat
     2821 cccaacagtt gtaactgtga tttattaaca gggaaacctg gatcgataaa aagtgtatag
     2881 ggctttttta cattggtacg agcagatgct aataaagctg caaaacttcc ttgcattgct
     2941 cctactgttg gaatacaatt taaaggactg acttcaagat ttatgaataa tttagcaaaa
     3001 cgtgctgtct ccgttttaaa aaatggaatt ccttcaatgt cggaataaag tgaggttaaa
     3061 cctttatcta atgcttcctt ttctgcctga acggtaacag gtaaagtggg caaacccggc
     3121 acacccattt ccatgtgaat aaattctata ccgctttgtt tttcaatttg agaaaccagt
     3181 ttaccgattt cacgaataga gagttgattg atatcagaag taccaaattg ggcaaatagt
     3241 gaatcaacaa gttccttatc aataatcatg attaaaaatt ctaataatat ctttatattt
     3301 tacggaataa ttgagttttt caaaaatatc aaatcgtata ttccatcaaa aaattatttg
     3361 ctttcttcct tttttcggtt atcaattacc ctgactgctt ttccttcgct tcgcggaatg
     3421 gaacgaggct caacaagtgt aactttgatg cttaaaccaa tagcactgcg aagggaatgt
     3481 tcaattcttt tcgatagact ttccagttca cgaatagtct ccaccatttt tgattcatcc
     3541 aattccactt tcaattccag ataatccagg ttatcttttc tatctacaca caaaagataa
     3601 tgggcactcg tttcgcacat ttccaacaaa acttgttcaa tttgagaagg aaataaattc
     3661 acaccccgaa taatcagcat atcatcactt ctgccagtaa tcttcttcat tctaaccagc
     3721 gtacgttcac attcgcattt ttcatgattg agcatagaaa gatcgcgcgt attgtatcgc
     3781 aaaagcggaa taccttcttt ggtcaatgtg gtaaaaacaa gttctccttc acaaccatct
     3841 ggtaatggct gtttggtttt cgggtcgaca atttctggaa aaaaatgatc ctcattgata
     3901 tgtaatcctt gatgaaaact acaatcatga gcaacacccg gtcccattac ttcacttaat
     3961 ccataaatat caaaagcctg gattccccat tttctttcca attcaagccg catgttttca
     4021 gtccatggtt ctgctccgaa aatacccaat cgcaatttca tatcggcacg tgaataacca
     4081 aattccgaca tcgcatctac cagatgaaga gcatacgaag gtgtacacgc aaggactgta
     4141 acaccataat cagacattaa ctgtaattga cgcttcgtat tccctcctga agctgggata
     4201 accgtagcac caattgtttc agctccatag tgcaaaccca agccacctgt aaacaaacca
     4261 tatccataag aaacttgcac cacatcttct tttgtcacac ctgccatcgt caagcagcga
     4321 gcaagtgatt cgcaccagtt ttgaatatca ttttttgtat aacctaccgt agtgggttta
     4381 cctgttgtgc cactggaagc atgtactcta acaatatctt tcattgggac agcaaaaagt
     4441 ccaaatggat aattgtcacg taaatcgttt ttataagtaa aaggcagttt ggaaatatca
     4501 tcaatggagt gaatatcaga gggagtaata ttcatttcct ccattttttt gcgataaaaa
     4561 ggcacatttc gatatactct gttcactaaa tttcttaaac gttcactttg aagttctcgc
     4621 atttcttcgt gcgacataca ctctacttcc tcattccaga tcataccgtc agttttaaat
     4681 ctgattcata aatgggtttg aattgatatt ttttaattac ttctgttgcc ttatccatat
     4741 tgttggttcg cattaccata atggatttgc ttccaaaact aaaagcataa agatattcaa
     4801 tagaaatgtt ttcagccgta aatttatcaa ggatgcgagc taatgagcca ggaacacctt
     4861 ccaattccaa agaaagaacg tcgtgaatgc ggacagaaca attttcagct ctgagtactt
     4921 ctactgcttt taacggatcg gacaccaaca tacgtaaaag gccataatcg ctgctatcgg
     4981 caagtgttaa ggcaataatg ttgatattat tgcgagccaa aatatttaaa atttcgttga
     5041 gatgaccagt tttgttctcc acaaaaacag aaagttgacg gattttcatt gcagaaataa
     5101 ttttaaaaat ttaaaaatgc aaatttacaa caaataataa aaataaacag cataaattta
     5161 gatagcttat ttggcgaagt gtatcgactc gtcaaagttt tgacacgagt aatggcaaat
     5221 gcaaaatatt atttgcgttt taaaacaact ttatccagaa tgttgagaaa aatagaaatt
     5281 gctaaagctg caagaaacac aattaatgtc accttgtcga ccagttcagc attaatattc
     5341 agccctgtaa ttctatcaaa aaggtatttg agataagaat ttggaagatt tgttttcccc
     5401 agtttttcca gtaccataaa atgccagtcg gtgagtggac aataacccgg tgtaccgaca
     5461 atcattccc
//
LOCUS       DAOSAS010000117         5437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_9488_length_5437_cov_13.728725, whole genome shotgun sequence.
ACCESSION   DAOSAS010000117 DAOSAS010000000
VERSION     DAOSAS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9488_length_5437_cov_13.728725"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..>1143)
                     /locus_tag="PLR72_06025"
     CDS             complement(220..>1143)
                     /locus_tag="PLR72_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOK36791.1"
                     /translation="LYGIHFTKNPKIPVYHPEVEAYEVTDKDGKFIAVLYTDFHPREG
                     KQSGAWMSEFKGQWKKNGKDSRPQIVIVMNFTKPTATKPALLTFDEVTTLLHEFGHSL
                     HGMLSDCTYPSLSGTNVYRDFVELPSQIMENWAFQKEFLDDFAAHYETNEKIPDELIN
                     KIKEAKNFHVAYACLRQLGFGYLDMGWHTLSTDFEGNVVQFEKNSMIDTQILPDVPNT
                     AISPTFTHIFSGGYAAGYYSYKWAEVLEADAFSVFEKNGIFDQGTANSFLENILKKGG
                     TEHPMILYKRFRGQEPTIDALLQRNGIVTNN"
     assembly_gap    1144..1243
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1244..1710)
                     /locus_tag="PLR72_06030"
     CDS             complement(<1244..1710)
                     /locus_tag="PLR72_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HOK36792.1"
                     /translation="MVTTSIASTALAGNPFFETYKTPHQTIPFNLIKNEHYIPAFEEA
                     IRQHNKEIETITNNSKSPTFKNTIEALEYSGRLLDKVSSPFFNLLSAETNDELQQIAE
                     KISPELTEHFNSIYLNDKLFQRIKIVYGQKDKLPLTPEQNMLLQQTYDAFVKNG"
     gene            complement(1788..3122)
                     /locus_tag="PLR72_06035"
     CDS             complement(1788..3122)
                     /locus_tag="PLR72_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK36793.1"
                     /translation="MKNIFFVIIIFLSPLITFYMKANEEESFITKLNVLDNDSIYKYV
                     YIYNNGKKVVETKYFQKNNEWFRYSQIEWTYEGENCTSQIERKWEDKKWNPTYSIEYH
                     YENNQLIDEIDYNYEQNSALAVYKTHFEYQNSKLISKKNYQFENDNWRLIQEINYHYA
                     TDEKIDSILLTFYVSENEKEEFLSTFGYLLDGLISTNLIQQKNDSGKWINSQLVQWFY
                     HPGTQQVATLITKKWDVNFTKWENYQKSEFKYVNEQPLTEIYQHWELAFWRNDFRYDY
                     FYDESGNLEKKVLSLPIYEKWRPAINIFYDNYTKKMSHVIKAQYDFWGGEVNEPLTSY
                     IPFLFNDEWTIKKAQYIEIDYDDFIDRTNTITNENNNIFIYPNPSEGIYYLNHSSAEI
                     QSWTVTALNGQMIISQKQPSSSVIDLTDCPQGIYILKMQTSDGQFIQKIIKK"
     gene            complement(3254..4030)
                     /locus_tag="PLR72_06040"
     CDS             complement(3254..4030)
                     /locus_tag="PLR72_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOK36794.1"
                     /translation="MILALIQKIETCDNLLKSASYLAVQLKKKFGILLINNKKEELIE
                     KEKSYLNSKMNSLQLLSQPDQIFFTTSGKELPEICEKVEALFLLIQLPDDSKKNIRFW
                     LSQCRELRIPYVLFKDSFSSLDLSRVIVPINFLEEEIEKAQFASAFGRFCGSKIIILL
                     ANDYGSKARQTAEKMKELFDKFQLNYTLEKGNADSFKIENEAVQLAIEENVGMVFVSA
                     SREYGLDDIVFGPKELHLVKKSPVPILLVNPRGDLYALCN"
     gene            complement(4061..>5437)
                     /gene="alr"
                     /locus_tag="PLR72_06045"
     CDS             complement(4061..>5437)
                     /gene="alr"
                     /locus_tag="PLR72_06045"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOK36795.1"
                     /translation="LILSDILQSGHSSEELYKTVAFLVKSKNIQQMIGIGSEISKYAD
                     EFNFEDKNFFETTEDFLKSSLIYTFQDKIILLKGSRKFQFEKILERLELVAYETTLEV
                     NLNALIENFNYFRSKLLPETKIMSMVKAFAYGSGSEEVARVLQHHHCDYLAVAVADEG
                     AELREEGIRIPIVVMNPEKGSFRMILDYQLEPEIYSFRILESFLTATEKLGIVDYPIH
                     IKIDSGMHRLGFDPSEVEKLISILKNQNQLKVRSVFSHLAASDEPAFDNFTRQQVETF
                     SSCADKFSAAFPHHILRHILNSAGIERFPEYQFDMVRLGIGHYGISSLPNIKLRQVCS
                     LKTIILQIKNVKKGETIGYGRKAMMKEDARIAVIPIGYADGFDRKLGNGVGEVLVNGK
                     TAKVVGNVCMDLTMIDVTHLDIKEGDVVEIFGEHLLISEISKKLNTIPYEVLTRVSRR
                     VKRVYIYE"
BASE COUNT         1828 a          885 c          760 g         1864 t
ORIGIN      
        1 gttaattcta aatcagaacc attgtaaaca ggatattcat cataagaaga ataatgggca
       61 accttgtgac aggaacataa aaagcctgga agaaaaaaca taaatatgat ttttttcatt
      121 tttaaatgat tgatttataa taaatttacg aacaatcttt aaaaaattta acaagtaaaa
      181 attctttttc aaaacataaa aacaattttc ttgaattctt taattattcg tcacaatgcc
      241 atttcgttgt aacaacgcgt caattgttgg ttcttgtcct ctaaaacgtt tatataaaat
      301 cataggatgc tctgttccac ctttttttaa aatattttcc aaaaaagaat ttgcagtacc
      361 ttgatcaaaa atgccatttt tttcaaacac agagaaagca tcagcctcca gcacttctgc
      421 ccatttataa ctataatagc ctgctgcata accaccagaa aaaatatgag taaaagttgg
      481 actaatggca gtattcggta catcaggaag aatttgcgta tctatcatag agtttttttc
      541 aaattgaacc acatttcctt caaaatctgt agataaagta tgccagccca tatcaaggta
      601 accaaatccc aattggcgaa gacaggcata agcaacgtga aaattcttgg cttcttttat
      661 cttattgatc agttcatcag gaattttttc gtttgtttca taatgagcag caaagtcatc
      721 aagaaactct ttttgaaaag cccaattttc cataatttgc gaaggcaatt ctacaaaatc
      781 ccgataaaca ttagtacctg aaagagaagg ataagtacaa tcgctcaaca ttccatgcaa
      841 tgaatgaccg aactcatgta gcaaagtagt tacctcgtca aaagttaata aagcaggttt
      901 ggtagcagta ggtttagtaa aattcataac aattacaatc tgtggacgac tatcttttcc
      961 attttttttc cattgtcctt tgaattcact catccaagcg ccactttgtt tcccctcacg
     1021 cggatgaaaa tcagtgtaaa gaactgctat aaattttcca tctttatcag ttacctcata
     1081 agcttctact tctggatgat aaacaggaat ttttggattt tttgtaaaat gaatcccata
     1141 aagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccatttt ttacaaaagc
     1261 atcataggtt tgctgtaata gcatattttg ttcaggagta agcggaagct tatctttttg
     1321 tccataaaca attttaatcc gctggaacaa tttatcattt aaataaatag aattaaaatg
     1381 ttcagttaat tctggtgaaa ttttttcggc tatttgttga agttcatcgt tagtttcggc
     1441 acttaataaa ttaaaaaaag gtgaactgac tttgtccagt aatctcccag aatattctaa
     1501 cgcttcaata gtgtttttaa aagtaggcga ctttgaattg ttggtaatag tttctatctc
     1561 tttattatgc tggcgaatag cttcttcaaa agcaggaata taatgttcgt ttttaattaa
     1621 gttaaaagga atagtttgat gcggtgtttt gtaagtttca aaaaaaggat tgcctgccag
     1681 agcagtagaa gctatggaag ttgtgaccat aataaaaaat tttacgattg aatgattttt
     1741 attaaatttt tgatttatct tttgaatcat tttttgaaaa caaaattcta ttttttgata
     1801 attttttgaa tgaattgtcc atcactggtt tgcattttaa gaatataaat tccttgagga
     1861 cagtctgtca aatcaattac agaagaagaa ggttgttttt gagaaataat catttgccca
     1921 tttaaagcag taactgtcca agattgaatt tcagcactcg aatgatttag ataatatatt
     1981 ccttccgaag gattagggta aataaagata ttattatttt cattggtaat cgtatttgtt
     2041 ctatcaataa aatcatcata atcaatttct atatactgag ctttttttat tgtccattca
     2101 tcattgaaca aaaaaggaat ataagaagta aggggttcat ttacttctcc accccagaaa
     2161 tcgtattggg cttttattac atgggacatt tttttggtat aattatcata gaagatatta
     2221 atagcaggac gccatttttc ataaattggc aaggaaagga ctttcttttc taaatttcca
     2281 ctttcatcat aaaaataatc ataacgaaaa tcgtttcgcc agaatgccaa ttcccaatgt
     2341 tgataaattt cagttaaagg ttgctcatta acatatttaa attcactttt ttgataattt
     2401 tcccattttg tgaaattaac atcccacttt tttgtaatta aagtagcaac ttgttgtgtg
     2461 ccaggatgat aaaaccattg aacaagctga ctatttatcc atttgcctga gtcgtttttt
     2521 tgttgaataa gattagttga gatcaagccg tctaacaaat atccaaaagt ggaaaggaat
     2581 tcctctttct cattttcaga aacatagaaa gtaagaagaa tagaatctat tttttcatca
     2641 gtggcatagt gataatttat ttcttgtatc aatcgccaat tatcattttc aaattgatag
     2701 ttctttttag aaattaattt agaattttga tattcaaaat gagttttata tacagctaat
     2761 gctgaatttt gctcataatt ataatcaatt tcatctatta attgattatt ttcataatgg
     2821 tactcaatag agtaagtcgg attccatttt ttgtcttccc attttctttc aatttgagaa
     2881 gtacaatttt ctccttcata agtccattca atctgagaat agcgaaacca ttcattgttt
     2941 ttttgaaaat atttggtttc taccactttc tttccattgt tatatatata aacatatttg
     3001 taaatagaat cgttatcaag cacattcagt tttgttatga aagattcctc ttcatttgct
     3061 ttcatgtaga aggttataag aggagataaa aaaattatga taacaaaaaa gatgtttttc
     3121 attgattttt cagagtatta atttgacaga ggctctcaaa attaagcata aaaatctaat
     3181 gttacaaata caaaaataca aaaagatatt agaatttttt gattaagaag gtgcctatat
     3241 aattgaaaac aagttaatta caaagagcgt acaaatcgcc acgaggattg actaacaaaa
     3301 taggtacagg agatttctta accagatgaa gttcttttgg accaaaaaca atgtcatcta
     3361 agccatattc tctggaagca gaaacaaaca ccattccaac attttcctca atagccaatt
     3421 gaacagcttc gttctctatt ttaaaactgt cggcattgcc cttttccaac gtataattta
     3481 attgaaactt atcaaacaat tccttcatct tttctgcagt ctgacgagct ttcgagccat
     3541 aatcatttgc cagtagtatt attattttac tgccacaaaa gcgtccaaaa gctgacgcaa
     3601 attgagcttt ttcaatttct tcttctaaaa aattaatggg aactattact cttgataaat
     3661 ctaaagacga aaacgaatct ttaaaaagaa catagggtat tcgcaactca cgacattgcg
     3721 aaagccagaa acgaatattt tttttcgaat catccggtaa ttgaataaga agaaacaaag
     3781 cttcgacttt ttcgcagatt tccggaagct cttttccaga agtagtaaaa aaaatctgat
     3841 caggttgaga aagaagttgt aaagaattca ttttagaatt taaataagat ttttcttttt
     3901 caattaattc ttctttctta ttgtttatca ataaaatacc aaatttcttt tttaattgaa
     3961 cggcaagata agaggcactc ttcaataaat tatcacacgt ttcaatcttt tgaattaaag
     4021 ctaaaatcat atttttttac aggaaaaagt ttagcaattt ttattcgtaa atataaactc
     4081 gttttacccg acgtgaaaca cgcgttaata cttcgtaagg aatggtattt aattttttgg
     4141 aaatttctga aataagtaaa tgttcaccaa aaatttctac aacatcacct tcttttatgt
     4201 caagatgggt cacatcgatc atagttaagt ccatgcagac atttccaact acttttgctg
     4261 ttttaccatt aactaaaact tcacctacac catttcccaa tttacgatca aaaccatcag
     4321 cataaccaat cggaatcact gcaattcgag catcttcttt catcatagct tttcttccat
     4381 aaccgatagt ttctcctttt ttgacgtttt taatttgtaa tataattgtt tttaacgagc
     4441 aaacttgcct tagcttaata tttggcaaag aactaatacc atagtgtcca attccaagtc
     4501 gtaccatatc aaattgatat tctgggaaac gttcaattcc agcagaattc aaaatatgac
     4561 gcaagatatg atgggggaaa gctgctgaaa acttatcggc acaactggaa aaagtttcta
     4621 cttgttgtct tgtaaaatta tcaaatgctg gttcatcaga agctgccaga tgagaaaaca
     4681 ctgaacgaac ttttaactga ttttgatttt ttagaataga aattaatttc tccacttcag
     4741 aaggatcgaa tcccaatcga tgcatgccac tgtcaatttt aatatgaatc ggataatcca
     4801 caatacctaa tttctctgta gcggttaaaa aagattccaa tatacgaaaa ctataaattt
     4861 caggttctaa ttgataatcc agaatcattc tgaaacttcc tttttctgga ttcattacaa
     4921 cgataggaat tctaatacct tcttctctta attctgcacc ttcgtcagca acagctacag
     4981 caagataatc acaatgatga tgctgtaaaa ctcttgccac ttcctctgaa ccgcttccat
     5041 aagcaaaagc ttttaccata ctcataattt ttgtttcagg taaaagtttt gaccgaaaat
     5101 aattgaaatt ttcaatcaag gcattcaaat ttacttccaa cgttgtttca taagctacta
     5161 attctaacct ttccagaatt ttttcaaatt gaaatttacg ggaacctttg agaagaataa
     5221 ttttatcttg aaaagtataa atcaaagaag attttaaaaa atcttccgtc gtttcaaaga
     5281 aatttttatc ctcaaaatta aattcgtcag catatttgga tatttctgaa ccaatgccga
     5341 tcatttgctg gatattttta cttttcacca gaaaagctac tgttttgtat aattcttctg
     5401 aagaatgtcc actttgtaaa atatcagaaa gaataag
//
LOCUS       DAOSAS010000118         5346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_9709_length_5346_cov_7.865054, whole genome shotgun sequence.
ACCESSION   DAOSAS010000118 DAOSAS010000000
VERSION     DAOSAS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9709_length_5346_cov_7.865054"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(551..2026)
                     /locus_tag="PLR72_06050"
     CDS             complement(551..2026)
                     /locus_tag="PLR72_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK36796.1"
                     /translation="MKQSFNLLLILLLGVQFLSSQVIKEFPIAVGSEAASALSAAFDG
                     TNYLVPIQGDDNSEYALTAQFISKAGELSGNRIDLNAYGPVAPIAIFDGTNYLLVWVD
                     AEHHLYARFLSPSGQWQGNAILIASDVIFDLRPFNVSYSDTNFLVAYVKSDFGLYGQL
                     LDKSGNLNGSSFFINNFGITDPQDEGSKEFNMAFDGTNYLIVWFKHSENENHSILGQF
                     VGKNGTLVGNAFTIDPPSPDHNPVSLIFDGNKYVISYDICFNPDSPYDCDDHGAYFRF
                     VSTSGTVGERFTLAETAGVHNAFTFVCSDGNNYLFTWESQSLSDNSADIYIKGRYYNM
                     SGTPLGEEFNIFTVPGSEGKIPLGGVMLFDGNNFLVGVTKAVLGETGFSDGDVYGVLV
                     QKYTSGLNNPQDNEKITLYNYPNPFANATTFYFQLPLRSKVSLIIYDAMGKQLMTVID
                     ETLDAGEHQIPWQANNLSGGFYFYKLQTNNFNETNKLVISK"
     gene            2376..4841
                     /gene="pheT"
                     /locus_tag="PLR72_06055"
     CDS             2376..4841
                     /gene="pheT"
                     /locus_tag="PLR72_06055"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOK36797.1"
                     /translation="MNLSYHWLQQYINIHLPVDELAEILTSIGLEVDSYEEISSVKGG
                     LEGLVIGEVLTCQPHENSDHLFVTTVNIGEVEPLQIVCGAPNVAAGQKVVVAPVGTKL
                     YAGEETFIIKRSKIRGVESYGMICAEDEIGIGTSHEGIIVLPDDAIPGTPAKEYYKIE
                     TDVVFEIDLTPNRADATSHFGVARDLAAYFSIRDKAIHLIKPSVENFKVQNQSLTIPV
                     IIENTEACKRYSAVTISGVKIKESPQWLQNYLKAIGIRPINNIVDITNFVLYEIGQPL
                     HAFDADKIKGGKVIVKNLPEGTTFITLDGVERKLSAEDLMICNAEEPMCIGGVFGGLD
                     SGVTETTQNIFIESAYFNPVSIRKTARRHGLNTDASFRFERGCDPENTIYALKRCALL
                     IQEIAGGEISSEIIDVYPKPIRPAEVRLLYEKIDNLIGEKIERSVINTILRALEIRII
                     EMNEEGYYLRIPLYRVDVQRDVDVIEDILRIYGYNRVEITERIVSTLSYWKKPDTFKL
                     QNIISEQLTSQGFNEVVNNSLTKSSYYVPLYSFPLENCVKILNPLSSDLSVMRQTMLF
                     GGLENIIYNIHRQNFDLKFYEFGNCYFYFPQNKRDNDILSPYREEFHLALWLTGNKHP
                     QSWTAPQEKTTVFELKAYIENIFLRLGIKRRQLHVQEFSDDLLSKALVYLNEKGKKIG
                     IFGIVHSNVLKKFDIEQEVYYADLDWNLLLALIKNHKISFTEIPKFPEVRRDLALLLD
                     KNINFSEIEKIAYETERKLLKRVQLFDVYEGENIAPGKKSYAVSFILQDETKTLTDDQ
                     IDAIMKKLMKNFAEKLGAQLRNK"
     gene            complement(4863..5168)
                     /locus_tag="PLR72_06060"
     CDS             complement(4863..5168)
                     /locus_tag="PLR72_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOK36798.1"
                     /translation="MIKHIVLFQLAEFAEGKTKKENAQFIKTELEKLINVIPQLKKIE
                     VGLNHPEAPQYNYDIALYCEFNSLNDLNIYQEHPAHKEVSEYVGKIKTSRVAVDYEV"
BASE COUNT         1835 a          831 c          882 g         1798 t
ORIGIN      
        1 gtcagtttag caaactgatg gtttcagcca ctcacccaca cttcctttta ttttttcaat
       61 gcaaagttaa aaaagtgttt tcatttttca aagtcttttc tcctttttgt taaggctttt
      121 cgtcaaattt taaaaataaa tatcaatttt gctcgatctt agcaaaaatt ttaactataa
      181 ggtaatgagc agaaataaac tttattttat aatagaagtt tctttttaaa aattccagta
      241 aaaaaaagaa gcattatatt taacaagata aagaagaaca ttacattaaa aataaaaata
      301 ttgttttttt tgactgtaaa aacataaaaa actattagaa gaatctttaa aatagggtta
      361 atttattgaa tgttcaaatt ggcctgtatg aggggatttg taaatttatt gaagtttaaa
      421 aatcaattcc tttatctcta attatttaat tatttgaatt ttaatcaatt gcaattttaa
      481 taaaattaga ggcgtaaaat tatacgcctc atgaattaaa aaattttatt ggtttattaa
      541 taactcgttt ttatttcgat attactaatt tattggtttc gttgaagtta tttgtttgaa
      601 gtttgtaaaa ataaaaccct ccactgagat tgtttgcctg ccacggaatt tgatgctctc
      661 ctgcatccaa agtttcgtca atcactgtca tcaattgctt tcccattgca tcataaatta
      721 tcaaggatac ttttgatcta agaggtaatt gaaagtaaaa agttgttgcg ttggcaaaag
      781 gatttgggta attataaagc gtgatttttt cattatcttg tggattattt aaaccagatg
      841 tatatttttg aaccaaaaca ccataaacat ctccatcgct aaatcctgtt tctccaagaa
      901 cagcctttgt aactccaacc aaaaaattat taccatcaaa caacattaca ccaccaagtg
      961 gaatttttcc ttcagaaccg ggaacggtaa atatattaaa ttcttcaccc aatggcgtac
     1021 ccgacatatt ataatatctt ccttttatat aaatatcggc agaattatcg gataacgact
     1081 gactttccca tgtaaacaga taattatttc catccgagca tacaaaagta aaagcattgt
     1141 gtacgccagc tgtttcagcc aaagtgaatc tttctcctac agtaccactg gtcgaaacaa
     1201 atctgaaata agctccgtga tcatcacaat cataaggact atcgggatta aaacaaatat
     1261 cgtaactgat aacatatttg tttccatcaa aaatcaaact taccggatta tgatcaggtg
     1321 agggcggatc gatagtaaaa gcatttccta ccaaagttcc atttttgccg acgaactggc
     1381 ctaaaataga atgattctca ttctcactat gcttaaacca aacaatcaga tagttcgttc
     1441 catcaaaagc catgttgaat tctttagaac cttcatcttg cgggtcggta atgccaaaat
     1501 tattgataaa aaaggaactt ccattcagat tccccgattt atccaataat tggccgtata
     1561 atccaaaatc cgattttaca taggcgacta aaaaatttgt atcagaataa ctcacgttaa
     1621 aaggacgtaa atcgaaaatc acgtctgaag ctattaaaat tgcgtttccc tgccattgac
     1681 cagaagggct caaaaaacga gcataaagat gatgttcagc gtccacccaa accaggagat
     1741 aattggtacc atcgaaaata gctattggag caactggtcc ataagcattt aaatcaattc
     1801 tatttcctga caactctccc gctttactga taaattgagc cgtaagcgcg tactcactat
     1861 tgtcatctcc ctgaattgga actaaataat tggtgccatc gaatgctgcc gacaaagccg
     1921 aagcagcttc actgccaact gcaattggga attccttaat aacttgtgag gaaagaaatt
     1981 gaactcccaa caatagaatc aaaagtaaat tgaatgattg tttcatggca ttaatttttt
     2041 taatgttaat tttttatttt ttatacaaag aatacaacag agaatttttt aaaaaaatga
     2101 atatgaattt gttgaaaaaa aatagttaag ctaaaaatta tcaataaatt atatttgcaa
     2161 atataagtac aatttttaaa aattgtactt tttttacatc tttttttgtt gttttttatt
     2221 tgttactttt tttatgttta aattttattt ttttacaaaa atcttttgct taagataaaa
     2281 atcaatttta tatgtctttt aaaaaactta tctttgcaaa ctataaaaaa tggaacaaaa
     2341 aaccctgttg ggttgattgt aataaattgt aaattatgaa tttatcatat cattggttac
     2401 agcagtatat taatattcat cttcctgttg atgaattagc agagatatta acttctatag
     2461 gcttggaagt ggatagttat gaagaaattt cttctgtgaa aggtggtttg gaaggattgg
     2521 taattggtga agttttaact tgccagccgc atgaaaattc tgatcattta tttgtgacga
     2581 cagtcaacat tggtgaagtt gaacctttgc aaattgtttg cggagctccc aatgtggctg
     2641 caggacaaaa ggtagttgtt gcaccagtgg gaacaaaact ttatgcaggt gaagaaactt
     2701 ttatcattaa gcgttctaaa attcgtggag ttgaatctta tgggatgatt tgtgccgaag
     2761 acgaaattgg tattggcacc agtcacgaag gaataattgt tttgccagat gatgcaattc
     2821 ctggtacgcc agcaaaagag tattacaaaa ttgaaactga cgttgttttc gaaatagatc
     2881 ttacaccaaa tagagcagat gctacttctc attttggagt agctcgtgat ctggctgctt
     2941 atttctctat cagggataaa gccattcatt taataaaacc ttccgttgaa aatttcaaag
     3001 tgcaaaatca gtctttaact attcctgtaa tcattgaaaa tacagaggct tgcaagcgtt
     3061 attcggctgt aactatttca ggagtgaaaa taaaagaatc accacagtgg ttgcaaaatt
     3121 atttaaaagc cattggcatt cgtccaataa ataatatagt cgatataaca aattttgttc
     3181 tatatgaaat agggcaaccg ttgcatgctt tcgatgcaga caaaataaaa ggtggtaaag
     3241 taatagtaaa aaatttgcct gaaggaacaa cttttataac actcgatgga gtagaacgta
     3301 agcttagtgc agaagatttg atgatttgca atgcagaaga gcccatgtgc atcggtggag
     3361 tttttggtgg attagattct ggggtgacag aaaccacgca gaatattttt attgaaagtg
     3421 cttattttaa tcctgtttca attcgtaaaa cagctcgtcg gcatggcttg aatacggatg
     3481 cctcttttcg ttttgaacgg ggttgcgatc ctgaaaatac catttatgcc ctgaaacgtt
     3541 gtgctttgtt gattcaggaa atagctggtg gtgaaatttc aagtgaaatt attgatgttt
     3601 atcctaaacc tattcgtcct gctgaagtta gactacttta tgaaaaaatt gataatttaa
     3661 ttggggagaa aatagaaagg agtgtcatca atactatctt gagagcactg gaaataagaa
     3721 tcatagaaat gaacgaagaa ggttattatt tgcgaatacc tctttatcga gtcgatgtgc
     3781 agcgtgatgt agatgtaatc gaagatattc tcagaattta tggatacaac agggtagaaa
     3841 taactgaacg cattgtttcc actttaagtt actggaaaaa accagatact ttcaaacttc
     3901 agaatattat ttcagaacaa cttacatctc aaggatttaa cgaagtagta aataattcac
     3961 ttactaaaag cagctattat gttccgcttt attcgtttcc acttgaaaat tgtgtaaaaa
     4021 ttctcaatcc tttgagttct gacttgagtg tgatgcgtca aacaatgctt tttggtggat
     4081 tggaaaatat tatttataat attcaccggc aaaatttcga tttgaaattc tacgaatttg
     4141 gcaattgtta tttttatttt cctcaaaata agcgtgataa tgatattctt tcgccttatc
     4201 gagaagaatt tcatctggca ctttggttga cagggaacaa acatccccaa tcatggacag
     4261 caccacaaga aaaaaccacg gtttttgaac taaaagctta tatagaaaat attttcttgc
     4321 gattaggaat aaaacgtcgt caacttcatg ttcaggaatt ttcggacgat ttgctaagca
     4381 aagccttagt ttatctaaat gaaaaaggga aaaaaatcgg aatttttggc attgtacatt
     4441 ctaatgtttt aaaaaaattc gatatagagc aagaagttta ttatgctgat ttggactgga
     4501 acttactttt agcattaata aaaaatcaca aaataagttt tacagagatt cccaaatttc
     4561 ctgaagttcg tcgtgatctg gcattactgc ttgacaaaaa tataaatttt tctgaaatag
     4621 agaaaattgc ttatgaaacg gaacgaaagc tattgaaaag agttcaattg tttgatgttt
     4681 atgaagggga aaatattgct cccggtaaaa aatcctatgc agtaagtttt attttgcaag
     4741 atgaaacgaa aactttaact gatgatcaaa ttgatgccat tatgaaaaag ctaatgaaaa
     4801 attttgctga aaaattagga gctcaattgc ggaacaaatg attaattttt ttattgctta
     4861 atttacactt cataatccac agctacacga ctcgttttta tttttccgac atattcagaa
     4921 acttctttat gtgccggatg ttcctgataa atatttaaat cgttcagtga attaaattcg
     4981 caatatagag caatatcata attatattga ggtgcttcgg gatgattgag ccccacttca
     5041 atttttttga gctgaggaat tacattaata agtttttcta attcggtttt gataaactgg
     5101 gcattttctt tcttcgtttt tccttcagca aattctgcca gttgaaaaag aacaatgtgc
     5161 tttatcataa aaatttaaaa gtttagatag atcaattttt ttaagctaaa tacttatttt
     5221 gaattattcc ttattaaatt taaaatgcta attatttgca tattacttta taacttttcc
     5281 cattgatacg taaaaaataa attccttgac gaactggaat aaaaaatcgt ccttgaacaa
     5341 gattgc
//
LOCUS       DAOSAS010000119         5335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_9729_length_5335_cov_12.868371, whole genome shotgun sequence.
ACCESSION   DAOSAS010000119 DAOSAS010000000
VERSION     DAOSAS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9729_length_5335_cov_12.868371"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..1520)
                     /locus_tag="PLR72_06065"
     CDS             complement(402..1520)
                     /locus_tag="PLR72_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOK36799.1"
                     /translation="MEIYPKLILDALSHVKYPGKGKDIVSLEMVQDNIRIEGNKVSFS
                     LIFDKPNDPFIKSVVKAAEQAILTYVSPEVDIKGNITPITKEVPKPAPVLPEVENIIA
                     IFSGKGGVGKSTIAANLAAALASLGYKVGLLDADIYGPSIPKMFGIEDARPFLENINE
                     KDMIIPVEKYGVKILSIGFFIDPESPLIWRGMMASNALNQLITDAYWGKLDYFIMDLP
                     PGTSDIHLTLVQTIGITGAIVVTTPQEVALTDARKAINMFRDEKINVPLLGLVENMSW
                     FTPAELPQNKYYIFGKEGGKKLAEEFNIPLLAQIPIVESIRVSSDAGEPLALNENRIL
                     STVFRELAENVVKNTNIRNATMAKTKKVEIIQKDKQKK"
     gene            complement(1567..2310)
                     /gene="trmB"
                     /locus_tag="PLR72_06070"
     CDS             complement(1567..2310)
                     /gene="trmB"
                     /locus_tag="PLR72_06070"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOK36800.1"
                     /translation="MSRNKLSKFADMNSFPHVFQFPAYPFYRSDTEFNLKGKWNKDFF
                     QNDFPIILELGCGKGEYTIGLAELYPEKNIIGIDIKGARMWTGAKYSFEKQMKNVAFL
                     RTNIEAVHYFFGENEVSEIWLPFPDPQMKKVRKRLTSTHFMKIYQQFVKPSGLIHLKT
                     DSNFMFTYTCEMVKINHYPVRYITEDVYNSDNNDPALKIQTFYESQWIERGLKIKYLQ
                     FNLEKRETFFEPDIEIERDTYRSFGRNKN"
     gene            complement(2312..2809)
                     /locus_tag="PLR72_06075"
     CDS             complement(2312..2809)
                     /locus_tag="PLR72_06075"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOK36801.1"
                     /translation="MKIKKISKLIIFFFVGIIGTACVHHETFLEYKTLPPQGWNKDSV
                     LTFQVNITDTSSLYNVYINIRNRGEYPYQNLWLFLNIQPPHSTLPIKDTIECYLADIH
                     GKWLGSGVGNVFEMPVLYKQNECFKQSGTYTFQIIQGMRNEVLPGISNVGLKVEKISS
                     PKKHN"
     gene            complement(2787..3986)
                     /gene="ricT"
                     /locus_tag="PLR72_06080"
     CDS             complement(2787..3986)
                     /gene="ricT"
                     /locus_tag="PLR72_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOK36802.1"
                     /translation="MKYKLDIGGYNINKKGCHRTSALKLSAIDWMCDLPESVKETDFV
                     EVQFKNTRKGYYLNSNKIPLEKGDMVAVEASPGHDIGEVTLVGKLVLLQMKKNKIDPE
                     KIEIKRIYRKAKETDLEKYKEAKNKEHSTMIKARQIAEKMNLKMKIGDVEYQGDGSKA
                     IFYYIADERVDFRELIKVFAETFHVRIEMKQIGARQEAGRIGGIGPCGRELCCASWMT
                     NFNSVVTSSARCQDLSLNPQKLAGQCCKLKCCMNYELDSYQDAIKDFPSKEIQLETLD
                     NTYFHFKTDVFKGLISYSTAQNMGTNLITISVQRAKEVIALNKQGIKPDKLEDQVSEE
                     IAQQEQNTENYVCQDNITRFDEKRNYHQHKNKGKNKKKQANSTNQSLKLRKNAENNRT
                     NENKEDK"
     gene            complement(4058..5212)
                     /gene="holB"
                     /locus_tag="PLR72_06085"
     CDS             complement(4058..5212)
                     /gene="holB"
                     /locus_tag="PLR72_06085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOK36803.1"
                     /translation="MLFSQIIGQKKIKERLLQSVNEQRIPHAQMLCGPEGIGKFALAL
                     AYAQYICCENKTETDACGVCPSCIKYQKLTHPDLHLVFPIIKQEKKKLVVCDDYVAQF
                     RKMVLDNPYFSENEWYAKIGSENKQGMIYTSESEEIIRKLNLKTYEAEYKVMIIWLPE
                     RMNLETANKLLKILEEPPEKTVFLLVSNSPDKIIPTILSRTQQIQIPRLNKEEIIEGL
                     IKNEHIAEEEAFNVAQISEGSYLKALSYLNEDDINKQNFEHFVKIMRLAWKLGNKKDY
                     AALKSLKNWAEEMATTSNGGRERQKNFLTYAQRMIRENFIFNLNEPALNYMTSFEKDF
                     SSGFSPFINERNVEELMNEFALAENHIERNVNPKMVFFDLLMKVVMLLKK"
BASE COUNT         1621 a          965 c          798 g         1951 t
ORIGIN      
        1 ggaatgtttt cgcctttgta aaatttgatt tcttgcatga taaaaatgat taataatata
       61 gaagttaatt tatttaatat aaaacagtag tattctaaaa agtgtgtaaa gaataatttt
      121 aattgaatgc aaaattagtc attttcccat tgaaatttca tcatttcata aataagatga
      181 ggaacagctt aatttgcaat ttctctatcc tttcattttg tgctttttat ttgattttcc
      241 acgttttttg aaacgttttt tgtgtcggcc aaaataatgt gggatttact caaacacgct
      301 acatattttt tatcttatca taaaaagtaa gcaaaaaatg gtcaactttt ttcaggacgt
      361 gacattaaca attcaaaact cccaacctcg caacttttca attatttttt ctgtttatct
      421 ttctggataa tctctacttt tttggttttt gccattgttg catttctaat gttcgtattt
      481 tttactacat tttctgccag ttcacgaaaa acagtagaaa gaatacgatt ttcattcagg
      541 gcaagtggct caccagcatc acttgatact ctgatacttt ctactatagg tatttgtgcc
      601 aaaagaggaa tgttgaattc ttctgctaac ttttttcctc cttcttttcc aaaaatataa
      661 tacttatttt gtggcaattc agcaggcgta aaccacgaca tattttcgac cagtcccaga
      721 agtggcacat tgattttttc gtcccgaaac atatttatag ctttccgtgc atcggtcaat
      781 gctacttctt gaggggtggt aacaacaatc gctccagtaa ttcctattgt ttgtaccaaa
      841 gttaagtgaa tatcactggt tccaggcggt aaatccatga tgaaataatc caattttccc
      901 cagtaggcat ctgtaataag ctgattaaga gcattacttg ccatcatacc tctccaaatc
      961 aacggacttt caggatcaat aaaaaagcct atagaaagaa tttttactcc atatttctca
     1021 actggaataa tcatatcttt ctcattaata ttttctaaga aaggacgagc atcttcaatt
     1081 ccaaacattt taggaataga aggaccgtaa atatcggcat ccaataaacc cactttgtaa
     1141 cctaaagaag caagagcagc cgctaaattc gctgcaatag tggatttccc tacaccaccc
     1201 ttcccagaaa aaatggcaat aatattttct acttctggca aaacgggagc tggtttgggt
     1261 acttctttgg taatcggtgt aatgttgcct ttgatatcca cttctggcga aacataagtt
     1321 aaaatagctt gttcagcagc tttcacaact gattttataa agggatcgtt cggtttatca
     1381 aaaatcagcg aaaaactgac tttgttccct tcaatacgaa tattatcctg taccatctcg
     1441 agagaaacaa tgtctttccc ttttccagga tattttacat gacttaatgc atcaagaatt
     1501 aactttgggt aaatttccat aattgaattt gaataatgtt gacctgatta atgtaaaata
     1561 cataaactaa tttttgtttc taccaaagct tctatatgta tcacgttcaa tttcaatatc
     1621 aggctcaaaa aatgtttctc ttttttctaa attgaattgt aagtatttaa tttttaatcc
     1681 tctttctatc cattgtgact cataaaaggt ttgaatttta agagcaggat cattgttgtc
     1741 agaattataa acatcttctg ttatatatct tacaggataa tgattaattt ttaccatttc
     1801 acaagtataa gtaaacataa aattgctgtc ggtctttaaa tgaataagtc cactaggttt
     1861 tacaaattgc tgataaattt tcataaaatg agtagaagtc aacctttttc gaactttttt
     1921 catttgggga tcgggaaaag gcaaccagat ttcacttact tcgttctcgc caaaaaaata
     1981 atgaacagct tcaatgttgg ttcgtaaaaa agccacattt ttcatttgct tttcaaacga
     2041 atattttgca cctgtccaca ttcgtgctcc tttgatatca atgccaatga tatttttttc
     2101 aggataaagt tctgccaatc caatggtgta ttctcctttc ccgcaaccca attcgagaat
     2161 aatgggaaaa tcattttgaa aaaaatcctt gttccatttt ccttttaaat tgaattcggt
     2221 atcacttcgg taaaaaggat aagcaggaaa ttgaaaaaca tgaggaaatg aattcatatc
     2281 cgcaaatttc gataatttat ttctgctcac tttaattgtg ttttttgggt gaagaaattt
     2341 tttcgacttt cagcccaaca ttgctgatgc ccggaagtac ttcatttctc attccctgaa
     2401 taatttgaaa agtataagtg ccactttgtt taaaacactc attttgcttg tataagacag
     2461 gcatttcaaa aacattgcct acaccggagc caagccattt tccatgaata tcagccaaat
     2521 agcattctat cgtgtctttg ataggcaacg tactatgtgg tggctgaatg ttaagaaaaa
     2581 gccataagtt ctgataagga tattcccctc tattacggat gtttatatag acattataaa
     2641 gtgaagaagt gtctgtgata tttacttgaa aagttagaac actgtcttta ttccagcctt
     2701 gtggaggtaa agttttgtat tccagaaaag tttcatgatg aacgcaagct gttcctatta
     2761 ttcctacaaa aaagaaaatt ataagtttac ttatcttctt tattttcatt tgttctattg
     2821 ttttctgcgt tttttctcaa ctttagtgac tgattggttg aatttgcctg ctttttttta
     2881 ttttttcctt tatttttatg ctgatgataa tttctttttt cgtcaaagcg agtaatatta
     2941 tcttggcata cataattttc tgtattttgc tcttgttgag ctatttcttc ggatacttgg
     3001 tcttccaatt tgtcaggttt aatgccttgc ttattgagag ctatgacttc ttttgctcgt
     3061 tgaactgaaa tagtaatgag gttagtcccc atattttgag cagtggaata agatattaat
     3121 cctttaaaca catcggtttt aaaatgaaaa taagtattgt ctaaggtttc caattgaatt
     3181 tcttttgaag ggaaatcttt tatggcatcc tgataagagt caagttcgta attcatacaa
     3241 catttcagct tacaacactg acctgccaat ttttgtggat ttaacgacag atcttgacaa
     3301 cgggcagagc ttgtgactac tgaattaaaa ttagtcatcc agctcgcaca acacaattct
     3361 cgtccgcagg gaccaattcc gccaatgcgt ccagcttctt gacgagctcc gatttgtttc
     3421 atttcaatac gcacgtgaaa agtttcggca aaaactttta ttaattctct gaaatctaca
     3481 cgttcatcag caatgtaata aaaaatagct ttactgccat ctccctgata ttctacgtcg
     3541 ccaattttca ttttcagatt catcttttca gcaatctgac gggctttaat catggtactg
     3601 tgttccttat ttttggcttc cttgtatttt tcaaggtcgg tttctttcgc ttttcgataa
     3661 atacgcttaa tttctatttt ctcaggatca attttatttt ttttcatttg cagtaacacc
     3721 agctttccaa ccagtgtaac ttctcctata tcatggccgg gagaagcttc tactgccacc
     3781 atatctcctt tttccaatgg aattttattg ctgttcagat aataaccttt tcgtgtgttt
     3841 ttaaattgaa cttcaacaaa atcagtttct tttactgact caggaagatc acacatccag
     3901 tcaattgcac tcaatttgag ggcactggta cggtgacatc cttttttgtt aatattatag
     3961 ccaccaatat ctaatttgta tttcatatct aaaataaact aaaaacaaga atttattaaa
     4021 aaatatcttt ttgaaaatgc caatgtgaaa ttacaattta ttttttcaac agcataacta
     4081 ctttcattag caaatcaaaa aataccattt taggatttac attacgttca atatgatttt
     4141 ctgctaaagc aaattcattc atcaactcct caacatttcg ttcgttgatg aaaggtgaaa
     4201 aacccgatga aaaatctttt tcaaaggaag tcatgtaatt caatgcaggt tcattcaggt
     4261 taaaaataaa attttcacga atcattcttt gagcataagt cagaaaattt ttctgtcgtt
     4321 cccttccgcc attcgacgta gtagccattt cttctgccca atttttcaag cttttcaaag
     4381 cagcataatc ttttttattc cctaacttcc aggctaaacg cataatcttc acaaaatgtt
     4441 caaaattctg cttattaata tcgtcttcat tcaaatagga taatgctttc aaataacttc
     4501 cttctgaaat ctgagctaca ttaaaagctt cttcttcagc aatatgttcg ttttttatca
     4561 atccttcaat aatttcttct ttatttaaac gtggtatctg aatttgctgg gttcgcgata
     4621 aaatagtagg aattatttta tcaggcgaat ttgaaaccaa aagaaaaacg gttttttcag
     4681 gtggttcttc gagaattttc aataatttat tggcagtttc aagattcatt ctttctggca
     4741 gccagataat cattacttta tattcagctt cgtaagtttt taaatttaat ttcctgatga
     4801 tttcttcact ttcgctggta taaatcattc cttgcttgtt ctcactacct attttagcgt
     4861 accattcatt ttcactgaag taaggattgt ccaggaccat tttccgaaat tgtgctacgt
     4921 aatcatcaca aactactaat ttttttttct cttgtttgat gatagggaat accagatgta
     4981 aatcaggatg agtgagtttt tgatatttaa tgcaggaagg acacacgccg caagcatctg
     5041 tttcggtttt gttttcgcag caaatatatt gagcataagc caaagccaat gcaaatttac
     5101 caataccttc aggtccacat agcatttgag catgaggaat acgctgttca ttaacgctct
     5161 ggagaagtct ttcttttatt ttcttttgac caatgatttg agaaaacaac ataataaaga
     5221 tttgaaaata tcctctataa aatgcaaatt tacaagataa ttttcaattt tcattcgatt
     5281 atgagaggtt aaaaaatact ttatgctgag ttggttcata tatttttgca aaata
//
LOCUS       DAOSAS010000120         5247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_9938_length_5247_cov_13.982666, whole genome shotgun sequence.
ACCESSION   DAOSAS010000120 DAOSAS010000000
VERSION     DAOSAS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9938_length_5247_cov_13.982666"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1396
                     /locus_tag="PLR72_06090"
     CDS             95..1396
                     /locus_tag="PLR72_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOK36804.1"
                     /translation="MQISEIQAVKQRFGIIGNSELLNRAIDIAVQVAPTDLSVLITGE
                     SGVGKENFPQIIHYYSQRKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGAMAERKGY
                     FEVADGGTIFLDEVGELPLSTQVRLLRVLEAGEFMKVGSSKIQKTDVRVVAATNFNIP
                     EALREGRFREDLYYRLNSVPIYIPPLRDRKEDIVLLFRKFAADFAEKYHMPPIRLTED
                     AKELLTNYYWRGNVRQLKNITEQISVIEHEREINAEILKAYLPKEEFERLPALLASHH
                     HNQKTFANEREILYQILFDMKQDMIELKKLVFNLMNGQKVEMQPEYFNVVPPYAPSSV
                     VAQSDNLITSPADFNLQKEDALTKNLPRNGKINTSVPYQEAQEYEELESIQQPLSLED
                     MERQMIKRTLEKHRGKRKAAAEELKISERTLYRKIKQYGLE"
     gene            1476..1943
                     /locus_tag="PLR72_06095"
     CDS             1476..1943
                     /locus_tag="PLR72_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOK36805.1"
                     /translation="MGVSCTVSYKFNGASIDYSKIKSISISDFPNMAELVYPPLSQEF
                     SEALRDIYTRQTQLKVLQQGGDLHLEGEIVDYQLTPMSISADTYAAETKLTISIKVRY
                     TNSKNPSDDFERTYSAFQTFDSSKMLTDVEEELVKEIITEIIENIYNDTVAKW"
     gene            1990..2676
                     /locus_tag="PLR72_06100"
     CDS             1990..2676
                     /locus_tag="PLR72_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36806.1"
                     /translation="MTYEEFIEFIKFPDHLTVENIFDLKELVNRYPFFVPARMLYVKA
                     LQLSNSIHLEQNIKNAVVYCSDRKWLYDFLFSSRKESLKSSPRPERKNLTGSYFDLLE
                     MTNNKQTGSKQSLKELAEQLKAARSSIIEKSPYKEQKNQRPLNINIETTKANKGENNF
                     QEKVANEQNLSCFNPITTEEKVKKLVKEKNYEEAVKILKELNLNNPEKSIYFADQIRF
                     LEKVIEYLKK"
     gene            2690..3082
                     /gene="secG"
                     /locus_tag="PLR72_06105"
     CDS             2690..3082
                     /gene="secG"
                     /locus_tag="PLR72_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOK36807.1"
                     /translation="MYLLFTILIVVASILLVLVVLVQNSKGGGLAAGFSSSNQFMGVR
                     KTTDFLEKATWTLIAFIVVFSVAAAGIHSNQTGGNKESEIKEQYQKAQKEVPGAVIPD
                     FGELPENAPSSPEKNNGGELPKIPTPEK"
     gene            3217..4527
                     /gene="murF"
                     /locus_tag="PLR72_06110"
     CDS             3217..4527
                     /gene="murF"
                     /locus_tag="PLR72_06110"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOK36808.1"
                     /translation="MEISELYQLFLRHPVICTDSRNCVPDSLFFALKGKNYDANIFAL
                     SALEKGCSYAVVDDKKYVIDERFILVKDVLQSLQELARFHRKQFDIPVIAITGTNGKT
                     TTKELISMVLMEKYNLLSTEGNLNNHIGVPLTLLQLKPEHQIAIIEMGANHPGEIKKL
                     AEIASPNYGLITNVGKAHLEGFGSLEGVMQAKAELYEYIYQEGEAIFINKKNQYLQQM
                     AEKAGFTDVSRKIEYCLNPIEKNVNITGEIVDCTPYLHMKCTTNEGTFEIKTKLIGDY
                     NAENVLAAAAVGNFFGVHNEQIKHGIENYQPQNNRSQFSVTQHNKLIIDTYNANPTSM
                     QAAILNFADMKFSPKTLILGDMLELGEQSEEEHQNIINLLQQKNFQNVFLVGEQFSKV
                     NKAYPSFENVEDLIIYLKQHPLFDQYILIKGSRGIYLEKVLPFL"
     gene            4581..>5247
                     /locus_tag="PLR72_06115"
     CDS             4581..>5247
                     /locus_tag="PLR72_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36809.1"
                     /translation="MKKKNLTIIIILSAVVLILGIFLIYNYKKTKEKEAEMAEMVEMM
                     NFEKEQLEKDYSDLALEYDTYSSKISNDSLVQLLQKEKMKVQQLLEELRITKATNAKR
                     IAELKNELTVLRAVMTNYVHQIDSLNAANKLLRTENIEVKRKYEQVNETAKQLAKEKE
                     TMKEVITRASMLEITNFSMTPLDSKNRKTQRYSKITTLQFNYTISKNPTTQPGNKTLY
                     LRIT"
BASE COUNT         1930 a          798 c          905 g         1614 t
ORIGIN      
        1 tttttttacc aataactttt cattattttt tactaatttt gtcagtagtt ttatgtcaga
       61 attacaggaa acatattaaa gaaaggaagt aagcatgcaa atttcagaaa tacaagccgt
      121 gaaacaacgg tttggcatca ttggaaattc agaacttttg aaccgagcta tagatattgc
      181 agttcaggtt gctccaactg atttatccgt tttgattact ggagaaagtg gtgtgggaaa
      241 agaaaatttt cctcagatta ttcattatta cagtcaacga aaacatggtc cctacatagc
      301 tgtaaattgt ggagctattc ccgaaggaac aattgattcc gaattgtttg gtcatgaaaa
      361 aggttctttt accggtgcta tggctgagcg aaaaggttat tttgaagtgg ctgatggtgg
      421 taccattttt ttggatgaag ttggcgaatt acccctttca actcaggttc ggctattgcg
      481 ggttctcgaa gcaggtgaat ttatgaaagt aggatcttct aaaattcaaa agactgatgt
      541 tagggtagtt gctgctacga attttaatat ccctgaagct cttcgtgaag gtcgtttcag
      601 agaagattta tattatcggc tcaattctgt tcccatttat ataccgcctt taagagatcg
      661 aaaagaagat attgtattgt tgtttcggaa atttgctgca gattttgctg aaaaatacca
      721 tatgccccct atcagattaa ctgaagatgc taaagaacta ctaacaaact attattggag
      781 aggaaatgtt cgtcagctta aaaatattac cgaacagatt tctgtaattg aacatgaacg
      841 agaaattaat gcagaaattt taaaggctta cttaccgaaa gaagaatttg aacgtttacc
      901 tgctttgtta gcttcacatc accacaatca gaaaacattt gcaaatgagc gagaaatact
      961 ttatcaaatt ctttttgaca tgaaacagga tatgatagaa ctgaaaaaat tagtttttaa
     1021 tttaatgaat ggtcaaaaag tagagatgca accagagtac tttaatgttg ttcctcctta
     1081 tgctccatct tcagtagtag ctcaatcaga caatttgata acatctcccg ccgattttaa
     1141 tctccaaaaa gaagatgctt taactaaaaa tttgccccgg aatgggaaaa taaatacttc
     1201 tgttccttat caagaagctc aagagtacga ggagctggaa tctatacaac agcctctttc
     1261 gttagaagat atggaacgac aaatgattaa gcggacactt gaaaaacatc ggggaaaaag
     1321 aaaagctgct gctgaagaat taaaaatttc agaacgaaca ttgtacagaa aaatcaaaca
     1381 atatggactt gaataatgaa aaaagaatga ataatttttc taacagattc ttttttataa
     1441 gtagatattt attgtttttt ttctttttat atgttatggg agtttcttgt accgtatctt
     1501 ataaattcaa tggagcttct attgattata gtaaaataaa atctatctct atttctgatt
     1561 ttcctaacat ggctgaatta gtttatccgc cgctctctca ggaattttca gaagcattgc
     1621 gtgatatcta tacccgccaa actcaattga aagttttgca gcaaggtggc gacctgcatc
     1681 tggaaggaga aattgtagat tatcaattaa caccaatgtc tatcagtgcc gatacttatg
     1741 cagcagaaac aaaattaact atttcgataa aagttcgtta tacaaattcc aaaaatccct
     1801 cggatgattt cgaaaggact tattctgcct ttcaaacttt tgacagctca aaaatgctca
     1861 ccgatgtgga agaagaatta gtaaaagaaa tcattactga aatcattgaa aatatttata
     1921 atgatacggt agcaaaatgg taaaaagttt tttaacaacg gttcattact tgaaataatg
     1981 aaatgaaaaa tgacgtatga agaattcatt gaatttatca aattccctga ccatctaacg
     2041 gttgaaaata tttttgacct gaaagaattg gtaaaccgtt atcctttttt tgttcctgca
     2101 aggatgcttt atgtaaaagc tttacagtta tcaaacagca ttcatctgga gcaaaatatt
     2161 aaaaatgccg ttgtatattg ttcggataga aaatggttgt atgattttct tttttcttcc
     2221 agaaaagaat ctttgaagtc ttctcctcgt cctgagcgaa aaaatttaac aggttcttat
     2281 tttgatttgc tcgaaatgac aaataataag caaactggtt ccaaacaatc attaaaagaa
     2341 ttagcagaac aattaaaagc tgcacgctcg tcaattattg agaaaagtcc ttacaaagaa
     2401 caaaaaaatc agagaccttt gaatataaat attgaaacca caaaagcaaa taaaggagaa
     2461 aacaattttc aagaaaaagt ggcaaacgaa caaaacttat cttgttttaa tccaataaca
     2521 actgaagaaa aagtcaaaaa actagtgaaa gagaaaaatt atgaagaggc agtaaaaatt
     2581 ttgaaggaat taaatttgaa taatccagaa aaaagtattt actttgcaga ccaaattcgt
     2641 tttttggaaa aggtgattga atatttaaaa aaataattat ccgatagtta tgtacctttt
     2701 gtttaccatt ttaattgtag tagcttccat attacttgtt ttagtagtat tggtacaaaa
     2761 ttcaaaaggt ggaggtctgg cagcaggctt ttcttcttcc aatcagttta tgggtgttcg
     2821 taaaaccact gattttcttg aaaaagcaac atggacactg atagccttta ttgtagtttt
     2881 ttcagttgcc gcagcaggca ttcattctaa tcaaacaggc ggaaataaag aatctgaaat
     2941 taaggagcaa taccaaaaag ctcaaaaaga ggtacctgga gccgtaattc ctgattttgg
     3001 cgaattacca gaaaatgcac cttcttctcc agaaaaaaat aatggtggtg aacttcctaa
     3061 aattcctacc cctgaaaaat aaagaaaaat tcaaatatta aatgtatttt aaagaggatg
     3121 gcaataaaaa caaccatcct tttttgttaa aatttatgtt aaccttcctt cagtttatca
     3181 gattatcttt atttttgtaa attattcatt cacattatgg aaatttcaga attgtaccag
     3241 ttatttcttc gacatcctgt tatttgtacg gatagtagaa attgtgtgcc tgactcatta
     3301 ttttttgctt taaaagggaa aaattatgat gcgaacattt ttgctttatc agctcttgag
     3361 aagggatgct cctatgcagt ggtggatgat aaaaaatatg ttattgacga acgttttatt
     3421 ttagtaaaag atgttttaca aagtttgcag gaactggctc gttttcatcg taaacaattt
     3481 gatatcccag ttatagctat cactggaacc aatggaaaaa ctacaacaaa ggaattgatc
     3541 agcatggttc tgatggaaaa atataattta ttgtccacag aaggaaattt gaacaatcat
     3601 attggcgttc cactcactct actgcaatta aagcctgaac atcaaatagc cattatcgaa
     3661 atgggagcca atcatccagg agaaatcaaa aaattagctg aaattgcttc tcccaattat
     3721 ggcttgatta ctaatgttgg aaaagctcat ctggaaggtt ttggatcatt ggaaggagta
     3781 atgcaagcaa aagcagaatt gtacgaatat atctatcagg aaggagaagc tatttttatt
     3841 aataaaaaga atcaatattt gcaacaaatg gctgaaaaag ctggttttac tgatgttagt
     3901 agaaaaattg aatattgttt gaatccaatt gaaaagaatg ttaatattac aggtgaaata
     3961 gtagattgta ctccttattt acatatgaaa tgcaccacca acgaaggaac atttgaaata
     4021 aaaacgaaat taattggtga ttacaatgct gaaaatgttt tagctgctgc tgcggtaggg
     4081 aatttttttg gtgtacacaa tgagcagata aaacatggaa tagaaaatta ccaaccacag
     4141 aacaatcgtt cacaatttag tgttactcaa cataataagt tgatcattga tacctacaat
     4201 gccaatccaa ccagtatgca ggcagctatt ttgaattttg cagatatgaa attctcgcca
     4261 aaaaccttaa tacttggcga catgttagaa ttaggagaac aaagtgaaga agaacatcaa
     4321 aacattatca atttgttaca acaaaaaaac tttcagaacg tttttctggt gggagaacaa
     4381 ttttccaaag taaataaagc ttatccttct tttgaaaatg tggaggattt gataatttat
     4441 ttaaagcagc atccgctgtt tgatcagtat attcttatta aaggctccag aggaatttat
     4501 ttagagaagg tattgccttt tttatagaat gacaacgaaa taattgattt tttagtagat
     4561 caaaaaatga attttgagac atgaagaaaa aaaacttgac tatcattatc attttatcag
     4621 cagtcgttct tatattagga attttcttaa tctataatta taagaaaacc aaagaaaaag
     4681 aagctgaaat ggctgaaatg gttgaaatga tgaattttga aaaagaacaa cttgaaaaag
     4741 actattcaga tttagctctg gagtacgaca cttattcttc aaaaataagc aacgactcac
     4801 tagtacaact gctgcaaaag gaaaaaatga aagttcaaca attgttggaa gaactaagaa
     4861 ttaccaaagc aacaaatgcc aaacgaattg cagaactaaa aaatgaattg acggtgctca
     4921 gagcagtaat gacaaattat gttcatcaaa ttgattcatt gaatgctgcc aacaagttat
     4981 taagaacaga aaatatagaa gtaaaacgaa aatatgaaca ggtaaatgaa actgccaagc
     5041 agcttgctaa agaaaaggaa actatgaaag aagtaattac tcgtgcttct atgctcgaaa
     5101 ttaccaattt cagcatgaca ccattggata gtaaaaatag aaaaacacag cgatacagca
     5161 aaattactac gttacaattt aattatacta ttagtaaaaa tcctacaact caacctggaa
     5221 acaaaacctt gtatttacga atcactc
//
LOCUS       DAOSAS010000121         5186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_10067_length_5186_cov_13.053401, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000121 DAOSAS010000000
VERSION     DAOSAS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10067_length_5186_cov_13.053401"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..1987
                     /locus_tag="PLR72_06120"
     CDS             356..1987
                     /locus_tag="PLR72_06120"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOK36810.1"
                     /translation="MTKEFKYQEPFPLGEDKTEYYLLTKDQVSVSQFEGQEILKVSPE
                     GLTLLARTAFRDCAFLLRPEHQKQVAAILKDPEASENDKYVALTMLRNAEISAKGVLP
                     FCQDTGTALVYAKKGNRVWTDGSDEEALSKGIYETYTQENLRYSQNAPLTMYEEVNTG
                     TNLPGQIDIMADQGEEYKFLFMAKGGGSSNKTFLFQETKALLNPETLEKFLAEKLKLL
                     GTSACPPYHIGFAIGGTSAEACLKTAKLASTKYYDELPTTGNELGRAFRDLELEEKIL
                     KAAQQLGIGAQFGGKYFAHDIRIVRLPRHGASCFVGLAVSCSADRNIKAKINREGIWV
                     EKLEDNPGKYIPEEYRQVGEEGGVKINLNQPMKDILATLTKYPVSTRLSLTGTMVVAR
                     DIAHAKMKEMLDAGYDLPQYMKDHPVYYAGPAKTPKGMPSGSFGPTTAGRMDSYVELF
                     QAHGGSLVMIAKGNRSQQVVDACKKYGGFYLGSIGGPAAILAQNNIKKVECIDFPELG
                     MEAVWKIEVVDFPAFILIDDKGNDFFKLIKPVVVC"
     gene            complement(2168..>2932)
                     /locus_tag="PLR72_06125"
     CDS             complement(2168..>2932)
                     /locus_tag="PLR72_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK36811.1"
                     /translation="LKVFETYLEIPTLPYMQNEIKIDTTYNMIKYFAAISATSAGIHD
                     KAIKLYEDLKDDDYETLSVYQLLAQEYYNINDTVNYIATLKEGFEKFPAEPWFLQNLI
                     NHYIYTGQIEEALKYLDAAIEKEPNVAQYYFVKGNLEENLGNIEEAKKAFNKALELDP
                     NFADAYASIGRLYYNQAVKMMEAANEIKDIKLYNAERDKINNIFKEAIPYFKKAVELK
                     PDDIEYKRNLRSLYYRLQMDKEAAEIDAEIKAMEND"
     assembly_gap    2933..3032
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3213..>5186)
                     /locus_tag="PLR72_06130"
     CDS             complement(3213..>5186)
                     /locus_tag="PLR72_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOK36812.1"
                     /translation="IAYIDNKEITIPELMKYLKAPDFPTGGIIYGYDGVKEAFETGRG
                     RIIIRGKTEIEVDASGHEKIIIKEIPYQINKVELIKIIASLVEDKKIEGISNINDESD
                     REGMRIVIDVKKDANSNVILNKLFKNTPLQSSFNVNNIALVNYRPYLLTLKDLIRHFV
                     NHRHTVITRRTKFELAEAEKRAHLLEGFMIILDNLDEAIQIIRDSKNPDEARTRLMER
                     FNLTEVQSRAIVEMRLRQLTGMEQDKLRAEYQEVLQLITHLKEILEKESLRMQIIKDE
                     LLEIKKQYGDPRRTEIVYSSEEFNPEDFYANEQMIITISHLGYIKRTPLSEFRAQGRG
                     GIGSKGSDSRDEDFIEYIYPASMHNTMLFFTQKGKCYWLKVYEIPEGSKNSKGRAIQN
                     ILNIDPDDKVNVFIRVESLSDPDYNQSHYLIFCTKKGVIKKTSLEAYSRPRQNGVIAI
                     QLRENDEVIQVRLTDGNSEIIMANRNGRAIRFHESNVREMGRTATGVRGMTLDEDGED
                     EVIGMICVNKDSGENILVVSEQGYGKRTLLDDENGEPIYRITNRGGKGVKTMNITEKT
                     GKLVAIKSVIDENDLMIINKSGITIRLKVSDIPVIGRATQGVRLINLEKRNDEIASVC
                     KVSSETNESQEFEIDEENEEENLLATSNEFLEN"
BASE COUNT         1574 a          870 c          863 g         1779 t
ORIGIN      
        1 aaatatttgt ttagcaaaac gtttgatttt atcagtggtt aaaatcaaga gattttattt
       61 ttagttcgac gtagccaaga ggctatgccg aacaatgatg ggacacagag attttttctt
      121 catttactca aaactcagaa ctttataatt tagttcatat aaaaatctac aaaaaattca
      181 cttttcattg gataaatttt tagattatga ttaaataaaa gggcaaagat tattttttaa
      241 aatttattca cccaatttta aatagaaaaa taattgtaag aatggattac agaaaaaatt
      301 ctttaatttt gtaggacctt taagtttatt ttttatcaat caagaaaatt aatctatgac
      361 aaaagaattc aaatatcagg aaccttttcc tcttggtgaa gataaaactg aatattattt
      421 gcttaccaaa gatcaggtat cagtatctca gtttgaagga caggaaattt taaaagtttc
      481 acctgagggg ttaacactat tagctcgcac tgcatttcgc gattgtgctt ttcttcttcg
      541 ccctgaacat caaaaacaag tggcggccat tcttaaagat ccagaagcta gcgaaaacga
      601 taaatatgtg gctttgacta tgcttcgcaa tgcggaaatt tctgccaaag gagttttgcc
      661 attttgccag gacacaggta ctgctcttgt ttatgcaaaa aaagggaatc gggtatggac
      721 tgatggtagt gatgaagaag ctctttcgaa aggaatttat gaaacctata cacaggagaa
      781 tttgcgttat tcgcagaatg ctccgctcac catgtatgaa gaagtaaata ccggtaccaa
      841 tctgcctggg caaattgata ttatggctga tcaaggcgaa gagtataaat ttctttttat
      901 ggcaaaggga ggtggctctt cgaataaaac tttcttgttt caggaaacga aagctttact
      961 gaaccctgaa actttggaaa aatttttggc tgaaaagttg aaattattag gaacttcggc
     1021 ttgcccacca tatcatattg ggtttgctat tggtggaact tcagcagaag cttgcttgaa
     1081 aactgctaaa ttagcatcaa ccaaatatta tgatgaactt ccaactacag gcaatgaact
     1141 tggcagagct tttcgtgatt tggaactgga agaaaaaatt ttaaaagcag ctcaacaatt
     1201 aggaattgga gctcaatttg gaggaaaata ttttgctcat gatattagga ttgttcgtct
     1261 gccacgacat ggagcttctt gttttgtggg tttggctgtt tcttgttctg ccgataggaa
     1321 catcaaggct aaaattaatc gagaaggaat ttgggtagaa aagctggaag ataatcccgg
     1381 aaaatatatt cctgaagaat atcgtcaggt tggcgaagaa ggtggcgtga aaattaatct
     1441 gaatcaacca atgaaagata ttttagccac attgacaaaa tatcctgttt ctactcgtct
     1501 gtcgcttaca ggtactatgg tggtagcacg cgatattgct catgctaaga tgaaagaaat
     1561 gcttgatgcg gggtatgatt tgcctcaata tatgaaagac catcctgttt attatgcggg
     1621 tcctgcaaaa actcccaaag ggatgccttc tggatcgttt ggccctacaa ctgctggacg
     1681 aatggattcg tatgtagagc tttttcaagc acatggtggt agtttagtga tgattgcaaa
     1741 aggtaatcga agtcaacaag tagtggatgc ttgtaaaaaa tatggtggtt tttatttggg
     1801 ttcaattggt ggacctgcag caatattggc acaaaacaac attaaaaaag tagaatgtat
     1861 tgattttcca gaattaggaa tggaagctgt ttggaaaatt gaagtggttg attttccagc
     1921 ctttattctg attgatgata aaggaaatga tttttttaag ttaatcaaac cagttgtggt
     1981 ttgttaaaaa aaattatgac cttctttcct tatttaaata tttcatttta gatcgaaact
     2041 tggtaataaa cgatagtttt gatttcaaaa tgtcctaaca aaagattttt tctgatttaa
     2101 caggaaacaa aaaaggcatc tgaaaaatat tagatgcctt ttttgtttta caatttagac
     2161 gaaaaaatta atcattttcc attgctttaa tttctgcatc aatttctgct gcttctttat
     2221 ccatttgcaa tctgtaatac aaagaacgca aattgcgttt atattctata tcatctggtt
     2281 tcaattccac tgctttttta aaataaggaa tagcttcttt gaatatattg tttattttgt
     2341 ctctctcggc attatatagt tttatgtctt taatttcatt agcggcttcc atcatcttaa
     2401 ctgcttgatt gtaataaagt ctgcctatac tagcataggc atcggcaaaa tttggatcca
     2461 attcaagtgc cttattaaaa gctttttttg cttcttctat gtttccaaga ttttcttcca
     2521 aattcccctt cacaaaataa tattgagcta catttggttc tttttcaatc gccgcatcca
     2581 agtattttaa ggcttcttct atttgcccag tataaatata atgattgata agattttgta
     2641 aaaaccatgg ttcagcgggg aatttttcaa aaccttcctt taaggtagct atgtaattaa
     2701 ctgtatcatt gatattgtag tattcttgag ccaacaactg atacacactt aatgtttcgt
     2761 aatcgtcatc ttttaggtct tcatatagtt tgattgcttt atcgtgtatg cctgcactcg
     2821 tagctgaaat agctgcgaaa tatttaatca tattataagt agtatcaatt tttatttcgt
     2881 tttgcatata gggtaaagtt ggaatctcaa gataagtttc aaaaactttc aannnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttcttcgc ttttgagaca ttgctctttt
     3061 gagcatagga aaaaacaaag cttactatca gaaacaaggt aagaatggac tttttcatat
     3121 tttcaataaa gttttttgtt tataattgat tgaataattt attagactgt aaaaatacag
     3181 tagatcaaaa gattgaacaa attcttttaa aattaatttt ctaaaaattc attagatgtt
     3241 gctaataaat tttcttcttc attttcctca tcaatttcaa attcttggct ttcattcgtt
     3301 tcagaagaca ctttacaaac agaagctatt tcatcgttcc gtttttcgag gttgatgagg
     3361 cgaacacctt gtgtggcacg accgataaca ggaatatcag aaactttcaa cctgatggta
     3421 atgcctgatt tattgataat cattaaatca ttttcgtcaa taacactttt gatggctacc
     3481 aatttaccag ttttttctgt gatattcatt gttttaacac ctttaccgcc tcgattagta
     3541 attctataaa taggttcacc gttttcatca tcaagcaaag tccgttttcc gtaaccttgt
     3601 tctgaaacca ccaaaatatt ttctccacta tctttattta cacaaatcat gccaattact
     3661 tcatcctctc catcttcatc aagtgtcatt ccacgaacac cggttgcagt acgtcccatt
     3721 tcacgaacat ttgactcgtg aaagcgaatg gctcgcccat ttcgattggc catgataatt
     3781 tcagaatttc catcggtcaa tcttacctga atgacttcat cgttttccct taactgaatg
     3841 gcaattacac cattttgtcg tggacgtgaa taagcttcca aacttgtttt tttgataacc
     3901 cctttctttg tgcaaaaaat caaataatgg gattgattat aatcaggatc agataagctt
     3961 tcgacgcgaa taaacacatt tactttgtca tcaggatcaa tgttcagaat attttgaatg
     4021 gctcttcctt tggaattttt acttccttct ggaatttcgt aaactttcag ccagtaacat
     4081 tttccttttt gggtaaaaaa tagcatggtg ttatgcatgg aagccggata aatatattct
     4141 ataaaatctt catctcttga atcacttcct tttgaaccaa tgccgcctcg tccttgcgca
     4201 cggaattcgc ttaatggtgt ccgtttgata tagccaagat gagaaatggt aataatcatt
     4261 tgttcattgg cataaaaatc ttctggatta aattcttcag aagaataaac aatttcagtc
     4321 cgacgtggat cgccatattg ctttttgatt tcaagaagct catctttaat aatttgcata
     4381 cgcaaagatt ctttttctaa tatctctttt aaatgagtta ttaattgtag cacttcttga
     4441 tactctgctc gcaatttatc ttgctccata cccgtaagtt ggcgtaatcg catttcaaca
     4501 atggctcttg actgaacttc cgtaagatta aaacgttcca ttaaacgagt gcgtgcttca
     4561 tcaggatttt ttgaatcacg aatgatttga atagcttcat ccagattatc caggataatc
     4621 ataaatcctt ccagtaaatg tgcccgtttt tcagcttctg ccaattcaaa cttggtacga
     4681 cgagtaataa ccgtgtggcg atgatttaca aagtgacgaa ttaaatcttt taatgtcaat
     4741 aaataaggtc ggtaatttac taatgcaata ttattgacat tgaaagagga ttgtaagggc
     4801 gtgtttttga atagtttatt taaaataaca ttggaattcg catccttctt aacatcaatt
     4861 acaatccgca tcccttcacg gtcagattca tcattgatat ttgaaattcc ttcaattttc
     4921 ttgtcttcta ccagagaagc aataattttt atcaactcaa ctttatttat ctgataggga
     4981 atttctttta taatgatttt ctcatgacct gaagcatcaa cttcaatttc agtttttcca
     5041 cgaataataa ttcgtcctcg tcctgtttca aaagcttcct tcacaccatc gtagccataa
     5101 atgatgccac ctgtaggaaa atcaggagct ttgaggtatt tcatcaattc tggtatggta
     5161 atttctttat tatcaatgta agcaat
//
LOCUS       DAOSAS010000122         5143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_10159_length_5143_cov_12.699292, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000122 DAOSAS010000000
VERSION     DAOSAS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10159_length_5143_cov_12.699292"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..387
                     /locus_tag="PLR72_06135"
     CDS             169..387
                     /locus_tag="PLR72_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36813.1"
                     /translation="MKTDLDLRSIHHKKDSLSITGNYPDYQYAKKLLLPIKNIFNETI
                     YIRWYTKPNKKVKTTYQASEYRYYPFVK"
     gene            complement(627..1652)
                     /locus_tag="PLR72_06140"
     CDS             complement(627..1652)
                     /locus_tag="PLR72_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOK36814.1"
                     /translation="MKKVFVIEILSIFFLTGFTQPQEKQNFKVVFYNTENFFDCFDDS
                     LTNDAEFLPDGTRGWTFAKYKKKQNNIGKVIASVGGWDAPALVGLAEVENERCLIDLT
                     HYSGLKNLHYKFLHFESPDPRGIDVALLYQPQFFKPLISKPIPIHFSTSPQIKTRDIL
                     YAAGIIPTDDTLHVFVCHFPSRLGGELESENKRVEVASILRTTIDSIYQVQNHPYIVI
                     MGDFNDYPTNKSLLKTLRALPPSDSIYSENLYNLMYPLHIKGKGSHKYNGEWGALDQF
                     IVSGALLKAGGKFYTLPMDAHFFEADFLLEDDKTNLGKQPFRTYVGMKYQGGFSDHLP
                     IYLDFWY"
     gene            complement(1662..3074)
                     /locus_tag="PLR72_06145"
     CDS             complement(1662..3074)
                     /locus_tag="PLR72_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36815.1"
                     /translation="MLNFLSCEKDNFTTNPLHTIEFSTDTITFDTLFTTVGSTTAKVM
                     VYNHNSKAVRINTIALAQGGNSFFRINVDGAVNENNFFSNVEIPAHDSIYIFVEVTID
                     ATDSDVPVLMEDEIVFLVNGIVKQIQLQAYGQEVEIFRDKIIWNDTLLTAKKPYLIYG
                     NLIIDTARTLTLEAGTKFYFHHNANLIVYGNLRAEGTFEHPILMRGDRFDKINFETPI
                     PYNVVAGQWGGIYLLGENGEHLLRHVKMNSGYVGIYLSNQNINSLPHLEINSCYIHNF
                     LRYGLVIQNANVDVINTEISNTSSYCVYLNGGTHSFIHCTIVNYFNNSNIQPVSRGSD
                     PAFMIMTLNRVAPMHTAIKNSVITGTLENEFSLVTRFLDEYKGTFSNNYIRKYEPLDL
                     PQFTNIRWYEKNDTVFKNIYYDYKKNNYFNFEPDSISPIRDLGDYTIATEYCPLDLNG
                     NNRLADGKPDAGAYEWKPTE"
     gene            3247..3552
                     /locus_tag="PLR72_06150"
     CDS             3247..3552
                     /locus_tag="PLR72_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36816.1"
                     /translation="MASRRKLKKRVNEISSNLLGEIYLRYLLFDIQDEEKFNQLLSNI
                     LLMRKEFISKINHIDGKNNPVMVKKYFRQFFSDWGTKITEIIEEIEKLSPVKQESEK"
     gene            complement(3635..4288)
                     /locus_tag="PLR72_06155"
     CDS             complement(3635..4288)
                     /locus_tag="PLR72_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOK36817.1"
                     /translation="MIIFAQGNLPYVDDKPYHFGFLVGLNTADFGIVHKDTLIDNKAY
                     HVSVSTLQPGFSVGIITNLRLSKYWNLRFTPTLHFSERTLTYRADTSSITTSTNINSI
                     PLCLPLYIKYSAERKDNYRPYLLGGGGIYYDFGRNKEKAILLKPTDYYVEFGVGCDLY
                     FSFFKLAPELKFALGFNNILTPLKERDAGGLTEQDKLHNNVLSKLTSKMLTLCFNFE"
     gene            complement(4684..5022)
                     /locus_tag="PLR72_06160"
     CDS             complement(4684..5022)
                     /locus_tag="PLR72_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36818.1"
                     /translation="MAQQIYNAAGKLVAKVDENCFYNSGGKLMGKVEGVFVYDNAGKL
                     MGKVDGVYIYDEKGKLIGKVDGNNLYDALGRKMATIIDKDLYDSYGRKIRRFNGLTQS
                     QIITYYYYFY"
BASE COUNT         1892 a          809 c          741 g         1701 t
ORIGIN      
        1 ttatatcatt gaagtaaaaa agtaaaaaga gactactacg gatatcatac taaccaaaaa
       61 gaaaagttcc gctgaaaatg aacagtttca gggaatttat tttatcaaac tcccttaaaa
      121 gcagaattag aagaaatcgt atgggtgata gaaaatactt ttagatgttt gaaaactgat
      181 ttggatttac gttctattca tcacaaaaag gattcattat caataacagg aaattatcca
      241 gattaccaat acgcaaaaaa gttgctatta cctataaaaa acatttttaa cgaaactatt
      301 tacattcggt ggtacactaa acctaataaa aaggtaaaaa ctacttatca ggcatcagaa
      361 tacagatatt acccatttgt caaatgaaaa tcgggaggac ataaatctga attcaaaaaa
      421 atcaattctc tcccttctgc aaataaacga tagttaactg caatgtgaac taaaagcaag
      481 ttgatcacaa caagtctgag ctaaattata atattaatag taacccccct tttgaactat
      541 tttactaacg ttcaatccag tttgtgggta aaattaatca ataccacaag gaatgaaaag
      601 tggtacgtcc gttagatagt ttgctttcaa taccaaaaat caagataaat gggtaaatga
      661 tcagaaaaac ctccttgata tttcataccg acataggttc tgaaaggttg tttacctaaa
      721 ttggttttat catcttccaa taaaaaatca gcttcaaaaa aatgagcatc cattggcaaa
      781 gtataaaatt ttcctccagc ctttaataag gctcctgaaa caataaattg atccagcgca
      841 ccccactcac cattatattt atgagagcct ttgcctttta tatgcaaagg atacataagg
      901 ttatataaat tttctgaata aatagagtcg cttggaggaa gtgctctcaa agtttttaat
      961 aaacttttat tggttggata atcattaaaa tctcccatga taacaatgta aggatgattt
     1021 tgcacctgat aaatgctatc aatcgttgtt cgtaaaatag atgccacttc tactcttttg
     1081 ttttcagatt ccaattctcc tcccagtcgc gaaggaaaat gacaaacaaa cacatgcaat
     1141 gtatcatccg ttggaattat tcctgcagca taaagaatgt cacgtgtttt tatctgtgga
     1201 gaagttgaaa aatgaatggg aataggttta ctgatcaatg gtttgaaaaa ttgcggttga
     1261 taaagaagtg ctacatcaat tccacgtgga tcaggtgatt caaaatgcaa aaatttgtaa
     1321 tgtaaatttt ttaaaccaga ataatgtgtc agatctatta gacacctttc attttctact
     1381 tcagctaaac ctaccaaagc gggtgcatcc caaccaccaa cagaagcgat tactttacca
     1441 atattatttt gttttttctt atattttgca aaagtccaac ctctcgttcc atctggcaaa
     1501 aattctgcat catttgtaag agaatcatca aaacaatcaa aaaaattttc agtattataa
     1561 aaaacaactt taaaattctg tttttcttgc ggttgagtaa aacctgttag aaaaaaaatt
     1621 gataatattt cgataacaaa cacctttttc atatagatgc attactcagt tggtttccat
     1681 tcgtaagctc ctgcatctgg tttgccatcg gctaagcgat tattcccatt caaatccaga
     1741 ggacaatatt cagttgctat ggtatagtcg cctaaatcac gaataggtga aatagaatca
     1801 ggttcaaaat tgaaataatt atttttcttg taatcataat aaatattttt aaacactgtg
     1861 tcgtttttct cataccaacg gatattggta aattgaggta aatccaaagg ttcatatttt
     1921 ctgatgtagt tatttgaaaa tgttcctttg tactcgtcca aaaaacgcgt taccagactg
     1981 aattcatttt ctaaagttcc tgttatgacg gaatttttaa ttgctgtatg catgggagcc
     2041 actcgattca gcgtcataat cataaatgca ggatcgcttc cccttgacac tggttgaata
     2101 ttgctgttat tgaaataatt tacaatggtg caatgaataa aagaatgagt accaccgttc
     2161 aaatatacgc aataactgct tgtattgctt atttcggtat tgattacgtc cacatttgca
     2221 ttttgaataa ctaaaccata tcgtaaaaaa ttatgaatat aacagctatt tatttctaga
     2281 tgaggaagag aattaatatt ttgattcgat aaataaatgc ctacatagcc actattcatt
     2341 ttcacatgac ggagcaaatg ttctccattt tcacctaata aataaattcc tccccattgt
     2401 ccagctacaa cgttgtatgg aataggtgtt tcaaaattaa ttttatcaaa acggtcgcca
     2461 cgcataagaa tgggatgctc aaaagtgcct tctgctcgca aatttccata aacaataaga
     2521 ttagcattat gatgaaaata aaattttgtt cctgcttcaa gcgttaatgt gcgagcggta
     2581 tctattatca gattaccata aattaaataa ggttttttag ccgttaataa agtatcgttc
     2641 cagatgattt tatctcgaaa aatttccact tcttgaccat aagcttgaag ttggatttgt
     2701 tttacaatac catttaccag aaaaacgatt tcatcttcca ttaaaacagg tacatcagaa
     2761 tctgtagcat cgatggtaac ttctacaaaa atataaatac tgtcatgagc tggtatctca
     2821 acattgctaa agaaattgtt ttcattaacg gctccatcca cattgatgcg aaaaaaagaa
     2881 tttcctcctt gagccagtgc aattgtattg atacggacgg ctttgctatt atggttataa
     2941 accattactt tcgctgtagt acttccaacc gtagtaaaaa gcgtgtcgaa agtgatggta
     3001 tcggtagaaa attcaatggt atgtaaagga ttagtggtaa aattgtcttt ttcacaagat
     3061 aaaaagttca gcatgagaaa aatagcaagg ggaaaaaaga aaaaatattt tttgtgaaaa
     3121 gagaacataa tgaaatgcat tataaaatgt tactgattta tttaggcaaa tttacactaa
     3181 aagatgaatt actcctaata aaaatttata tttgtagcac aatattaaat taaaaaatta
     3241 aaaaatatgg caagccgaag aaaattgaaa aaaagagtca atgaaatttc ttctaattta
     3301 ttaggtgaaa tttatctgcg ttatttattg ttcgacattc aggatgaaga aaaatttaat
     3361 caattgttat ctaatatctt attgatgaga aaagaattta tttcaaaaat caatcacatt
     3421 gatgggaaaa ataatcctgt aatggtaaag aaatatttcc gacaattttt ttctgattgg
     3481 ggcacaaaaa ttactgaaat tattgaagaa attgagaaat taagtcctgt aaaacaggaa
     3541 agtgaaaaat aatctttttc ttcactttat tgcgtaattt gtctaaccaa aaagttgcat
     3601 ttgtgacttg aattcagttt gattaaaaat tgctttattc aaaattaaaa cataaggtca
     3661 acatttttga agtcaattta gacaatacat tattgtgtaa tttatcttgc tcagtcaagc
     3721 cacctgcatc tctttccttt aaaggagtta agatattatt aaaccccaga gcaaatttca
     3781 attcgggagc aagcttaaag aatgaaaaat acaaatcgca gcctacgcca aattcaacat
     3841 aataatctgt tggttttaat aaaatagctt tttctttgtt tcgtccaaaa tcataataaa
     3901 tccccccacc gccaagtaaa taaggacgat aattatcttt tctttcagca ctatatttga
     3961 tatataaagg aaggcataga ggaattgaat taatatttgt agaagtagta atagaagagg
     4021 tatcagcccg ataagttaaa gttctttccg aaaaatgaag agtaggagta aaacgaagat
     4081 tccagtattt acttaatcgt aagttagtta ttatgcctac cgaaaatcct ggctgcagtg
     4141 ttgacactga aacgtggtaa gctttattat caataagcgt atctttatgt acgattccaa
     4201 agtctgcggt attcaatcct actaaaaatc cgaaatgata aggtttatca tccacatagg
     4261 gcaaattacc ttgtgcaaaa attatcataa aaggaaaaag gaagaaaaaa agaaaaagat
     4321 ttttttttct tgacgagaaa attagtaaaa tttttatagc atctttcaaa taaagaatat
     4381 tatttaactt tttgtattga atcatttgat aataaataaa ttaattaaaa aaagaaaaat
     4441 ataaaagcta ctactttttc tttattttga aaaaacgctt aaaataaatg attattgttt
     4501 atacttttac aagtatacaa aagaagataa taaaaaacct ctttataaac ttaagcactt
     4561 gacaatttca gttttttaat tatcacttaa cacaataggt gagatttttt aataaatttt
     4621 tcaaattact attagtaaaa accgatgaag attttttaag ataaaagtaa ttcttagaat
     4681 tatttagtaa aaataatagt agtatgtaat tatctggctt tgtgtcaacc cattaaatct
     4741 cctaattttt cgaccatagc tatcatataa atctttatct ataatagttg ccatttttct
     4801 gcccagagca tcgtataaat tatttccatc tactttacca attaatttac ctttttcatc
     4861 ataaatataa acgccatcca ctttccccat taattttcct gcattatcgt aaacaaaaac
     4921 gccttctact tttcccatta attttcctcc ggaattataa aagcaatttt catcaacttt
     4981 tgccactaat tttcccgcag cattataaat ttgctgtgcc attaggaatg aattagtaaa
     5041 taaaaataaa ataagcaaaa tttttttcat agaatgaaaa aatattattt tttacaaaga
     5101 aattaaaaat aatctatttc cccaaataat ttttattttt tat
//
LOCUS       DAOSAS010000123         5007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_10551_length_5007_cov_12.692246, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000123 DAOSAS010000000
VERSION     DAOSAS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10551_length_5007_cov_12.692246"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            395..2263
                     /locus_tag="PLR72_06165"
     CDS             395..2263
                     /locus_tag="PLR72_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK36819.1"
                     /translation="MKNKQTRNVSPWAWVPTLYFAEGIPYVVAMTVTVIMYKRLGISN
                     ADIALYTSWLYLPWVIKPFWSPFVDIIKTKRWWILSMQLLIGAGLAGVAFLIPVPFFF
                     QATLAVLWLIAFSSATHDIAADGFYMLALDSSQQSFFVGIRSTFYRFAMITGQGLLII
                     LAGALESHTGTKPVLLTVASTNSSQIEMMEIPENVLPADTAELSFVAYPSQLVLSTQT
                     ISRDSLNKILQYATEQNITNRFVEKEQKTVTGKSEKNSWWKRNVSDPLANYIRDHFGE
                     KREIKTDLTERVGNAGLVQIRLTKKPEKGKSLIFNITHAKGDKSISLVTGERLVFDET
                     NWDKPAYVVFQLDPKLKDEVKSEFKGVSGNVKLAWSITFFIIAGFFILIYFYHRFILP
                     YPDSDHPTLTNTAKDILREFGRTFATFFQKKGIGLAIAFMLLYRLGEAMLVKMASPFL
                     LDPREIGGLGLTTGQVGLVYGTVGVVALTIGGIIGGIVASQKGLKFWIWPMALCITLP
                     HLVYLYLSSVQPENMLLINLAVAIEQFGYGFGFTAYMLYLIYFSEGEHKTPHYAICTG
                     FMALGMMLPGMIAGWLQELLGYHHFFIFVVLCAIPTLVVIPFMKIDKEFGKKKTTT"
     gene            2362..3135
                     /locus_tag="PLR72_06170"
     CDS             2362..3135
                     /locus_tag="PLR72_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOK36820.1"
                     /translation="MKILILGAGKMGTFFTDLLCLHHEVALFDIDPKKLRFVFNTYRL
                     TDYEQIREFQPELVLNCVTLKFTIEAFQNVMPFLPENCMLSDIASVKTGLKEFYESTG
                     KRFVSTHPMFGPTFATLDNLSSQNAIIISESDFLGKTFFRDLYHSLHLKIFEYSFEEH
                     DKTTAYSLAIPFASTLVFAAVMKHQEAPGTTFKRHMEIAEGLLSEDDYLLTEILFNPY
                     TPEQIKKIQAELATLLDIIEKKDTERMQAFLEKVRKNIQ"
     gene            3410..>3750
                     /locus_tag="PLR72_06175"
     CDS             3410..>3750
                     /locus_tag="PLR72_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36821.1"
                     /translation="MEVFRNELKKLLDTISQLNNQGQDEENFKTPIIHFLTETYYKGK
                     NYVNSSKKYDLVIGNGEKVADSVAVIIETKRPSNSTEMITSTNPNAKALHELVHYYMQ
                     ERKNGNDELKYL"
     assembly_gap    3751..3850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3851..>5007
                     /locus_tag="PLR72_06180"
     CDS             <3851..>5007
                     /locus_tag="PLR72_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOK36822.1"
                     /translation="EFYNELLYILGLEEKSENNKIVIERRSKDRSEASLIENTINILK
                     TRDKLKGIDDITQFGQTEEEQLFSVALELCITWLNRILFLKLLEGQLIKYHNNDSNFA
                     FLSPDKVKEFDKLDELFFEVFAIKPAERTQKIQEQYAHIPYLNSSLFEMSLLEQQTIS
                     IANLKNDQQLPIYNKSVLKIATNKTEINTLQYLLQFLSSYNFSSDSHAKIQEDNRTII
                     NASVLGLIFEKINGYKDGAFFTPGFITMFMSRETIRKAVVQKFREKENKEFNDFDDVK
                     NYCRLHHKKEDIERFNDHINSLKICDPAVGSGHFLVSVLNELIAVKSELNILSYNGII
                     INWEVSVDNDELTVTHKFNNQPYEYFLMDDEKPAPDAQEVQMTLFNEKRLL"
BASE COUNT         1580 a          828 c          896 g         1603 t
ORIGIN      
        1 ttcaagtaaa aatacagttc aaaatttaat gaattaactc ttgcttctta cgaatttaat
       61 ttttctttta tatcaattta ttttttctcc ttataccatt gataaccaat tttataatat
      121 cgtgctcttc ttattaaatt gctgcaaaaa attaattttt ttgaagaatt tcttttgaat
      181 ttggtgacga ttgtttacat ttgtctcgca atttataaag aatcagtttt ataaaaattg
      241 taattgacat tcctgatttg ttgagaacaa atcgttgtat atttgagaaa aggaaccatt
      301 tgattttttt cattatcagc attttaacct tccaaaatga aaagtttgtt tgttaaatag
      361 aagaaaaaat aattttaata aatattacta agcaatgaaa aacaaacaaa ccagaaatgt
      421 ttctccgtgg gcttgggttc caactttgta ttttgctgaa ggcattcctt atgtggtggc
      481 gatgacagtt acggtcatta tgtacaaacg tttaggtata tcaaatgccg atattgcctt
      541 gtacacaagc tggttatatt tgccgtgggt gatcaaacct ttttggagtc cttttgtgga
      601 tattattaaa acgaaacgct ggtggatact ttccatgcaa ttgttaattg gtgcgggatt
      661 ggctggcgtt gcttttctca ttcctgtgcc tttctttttt caagccacct tagcggtgtt
      721 atggcttatt gctttcagtt ctgctaccca cgatattgca gccgatggct tttatatgct
      781 ggcactggat agttctcaac aatctttttt tgtagggata cgaagcactt tctatcgttt
      841 tgccatgatt accgggcaag ggttgcttat cattctggct ggtgcattgg aaagtcatac
      901 agggacaaaa cctgttttac tgacagttgc ttctactaat tcttcccaaa tagaaatgat
      961 ggaaattccg gaaaacgtac ttcctgctga tacagcagaa ttgtcgtttg tggcttatcc
     1021 ttctcagttg gtgttgagca cacaaaccat cagtcgggat tcgttgaata aaattcttca
     1081 atatgcaact gaacagaata tcacgaatcg ttttgtagaa aaagaacaaa aaacagttac
     1141 aggaaagtca gagaaaaatt cctggtggaa aagaaatgtg tctgatccac ttgcaaatta
     1201 tattcgagac cattttggag aaaaacgaga aataaaaact gatttaacgg aaagagtggg
     1261 taatgctggc ttggttcaaa tccgtttaac aaaaaagcca gaaaaaggaa aatcattgat
     1321 attcaatata acccatgcaa aaggtgacaa aagtatttcg ctggtcacgg gtgaacgatt
     1381 ggtttttgat gaaacaaact gggataaacc ggcttatgta gtatttcagt tagacccaaa
     1441 attgaaagat gaagtaaaaa gtgagttcaa aggagtttca ggaaatgtaa aattggcttg
     1501 gagcatcact ttctttatca ttgcagggtt ctttatcttg atttatttct atcatcgttt
     1561 tattttgcct tatcctgatt cagaccatcc tactttaaca aatacagcaa aagatatctt
     1621 gcgggagttt ggaagaactt ttgccacttt tttccagaaa aagggaatcg gtctggctat
     1681 tgcctttatg cttctttacc gtttaggcga agcaatgtta gtaaaaatgg cttcaccttt
     1741 tttgctggat ccgcgcgaaa ttggagggtt aggattgact accggtcagg ttggactggt
     1801 ttatggaacg gttggcgttg ttgctttgac aattggtggc attattggcg gtatcgtcgc
     1861 ttcacaaaag ggattgaaat tctggatctg gccaatggct ttgtgcatca cgctacctca
     1921 tctggtgtat ttatatcttt cctcagtaca acctgaaaac atgttgttaa tcaacctggc
     1981 agtcgcaatt gaacaatttg ggtatggttt tggctttact gcttatatgt tgtacttgat
     2041 ttatttctcg gaaggtgaac ataaaacacc tcattatgct atttgtacag gatttatggc
     2101 attgggcatg atgttgccag gtatgattgc tggctggtta caggaattgt taggctatca
     2161 tcatttcttt atttttgtag tactttgtgc tattccaacg ctggttgtca ttccctttat
     2221 gaaaattgac aaagaatttg ggaagaaaaa gacaacaact taatttcaat aattttatca
     2281 ggaacaattt tccttacgta tcgtgttttc ttttttaatt ttatttctac ttttgtagaa
     2341 aatataattt tattttagaa aatgaaaatc ttaatacttg gtgccggtaa aatgggcact
     2401 ttttttacgg atttattgtg tctccatcat gaagtggctt tattcgatat tgatcctaaa
     2461 aaacttcggt ttgttttcaa tacttatcga ctgactgatt atgaacagat cagggaattt
     2521 cagccagagc tggtgctgaa ttgtgttaca ttgaaattta ccattgaggc ttttcaaaat
     2581 gtgatgcctt ttctaccgga aaattgcatg ctttccgata ttgcttcagt aaaaacaggg
     2641 ttgaaggaat tttacgaaag tacgggtaaa cgttttgtat ccacacatcc tatgtttggt
     2701 cctacttttg caacactgga taatttgagt tcccagaacg ccatcattat ttctgagtct
     2761 gattttctgg gaaaaacttt tttcagagat ttgtatcatt ccctacattt gaaaattttt
     2821 gaatattcct ttgaagagca cgataaaacg actgcttatt cacttgctat tccttttgct
     2881 tccacattgg tttttgctgc tgtgatgaaa catcaggaag cgcctggaac tacttttaaa
     2941 cggcatatgg aaattgcaga aggtttgctg tcagaagatg attatttatt aaccgaaata
     3001 ttattcaatc cttatacacc tgaacaaatt aaaaaaattc aagctgaatt ggctactttg
     3061 ctggacatta ttgaaaagaa agatacagaa aggatgcagg cttttctgga aaaagtaagg
     3121 aaaaatatac aataggtgac taagcttcaa ttgtttccaa agacaaaaag tgttttttga
     3181 gttattatct tcaagttcag aaattataaa gtttgattaa agatatttct tgacagatga
     3241 ctttttaaac gctgaacaca ttttttgtac cctgacctca aaaaacattt tgaaaaacaa
     3301 ccttttaatt ctacatttgt agaaaaacag agccatgata ttgtcaacct tccctataga
     3361 aaaaagctta aatagggctt acttgaagca agaccttacc aaaaagcaaa tggaagtttt
     3421 caggaatgaa cttaaaaaat tgctggatac catttctcaa ttaaataatc aagggcaaga
     3481 cgaagaaaat tttaaaacac ccatcattca ttttctgaca gaaacttatt acaaaggcaa
     3541 aaactatgtg aatagttcaa aaaaatatga tctggtaatc ggtaatggtg agaaagttgc
     3601 cgacagtgta gcggttatta ttgaaaccaa aagaccctcc aattccaccg aaatgataac
     3661 ttctaccaat cctaatgcta aagccttgca cgaattggtg cattattaca tgcaagaaag
     3721 gaaaaacgga aatgatgaac tcaaatatct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn gaattttata atgaattgct ttacattctg gggctcgaag aaaaatcgga
     3901 aaacaataaa atagttatcg aacgccgaag taaagacaga agcgaagcca gcttgataga
     3961 aaataccatt aacattctca aaaccagaga taaattaaaa ggaattgacg atattaccca
     4021 atttggacaa acagaagaag aacagctttt cagcgtagca ctcgaattgt gcatcacctg
     4081 gctgaacaga attctgtttt taaaactgct ggaaggacaa cttataaaat atcacaacaa
     4141 cgattcaaat tttgcttttc tatcgcccga taaagtaaag gaatttgaca aacttgatga
     4201 attgtttttt gaagtttttg ccataaagcc tgctgaacgt actcagaaaa ttcaagaaca
     4261 atacgcacat attccttatc tgaacagctc gctttttgaa atgagtctgc tcgaacaaca
     4321 aaccatttcc attgccaatc tgaaaaacga tcagcaattg ccgatttaca acaaatcagt
     4381 tcttaaaatt gctaccaaca aaacagaaat aaacactttg caatatttac tgcaattcct
     4441 ttcttcctat aacttttcga gcgacagcca tgctaaaatt caggaagata accgaaccat
     4501 tatcaacgct tctgtgcttg gattgatttt tgaaaagata aatggttaca aagatggcgc
     4561 cttttttacg cccggcttca tcaccatgtt tatgagccgt gaaaccattc gcaaagctgt
     4621 ggttcaaaaa ttcagagaaa aagaaaacaa ggaatttaat gatttcgacg atgtaaaaaa
     4681 ttattgtcga ttacatcaca aaaaagaaga tattgagcga tttaacgatc atatcaattc
     4741 cttgaaaatt tgcgatccgg ctgttggctc tggacatttt cttgtctcgg tgctcaacga
     4801 actaattgcc gtgaaaagtg aattgaacat tctttcttac aacggcatca ttattaattg
     4861 ggaagtgagc gtggacaacg atgaattgac tgttacccat aaatttaaca atcagcctta
     4921 tgaatatttt ctgatggatg acgaaaaacc tgctcctgat gctcaggaag tacaaatgac
     4981 tttattcaat gaaaaacgat tgctcat
//
LOCUS       DAOSAS010000124         4926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_10805_length_4926_cov_9.447547, whole genome shotgun sequence.
ACCESSION   DAOSAS010000124 DAOSAS010000000
VERSION     DAOSAS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10805_length_4926_cov_9.447547"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLR72_06185"
     CDS             <1..211
                     /locus_tag="PLR72_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36823.1"
                     /translation="TSYDFVAKQFTYTNVSVSRDLHCWNISANFVPFGPFQSYYFRIG
                     VNSSMLRDLKYEKRSGYGSTPIDWY"
     gene            319..891
                     /locus_tag="PLR72_06190"
     CDS             319..891
                     /locus_tag="PLR72_06190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOK36824.1"
                     /translation="MKIDVNKVVSAKYELFVEGENEGELKLMERATEQEPLSFIYGVG
                     MILPKFEEYLYGLEAGDDFEFTIDSEDGYGHYDKEDIIDLDRSIFETDGKLDNNVIFE
                     GNVVTLMDNEGNRLPAKIVTITENYVTVDLNHPLADEKLHFKGTVLEVRDATEEELSS
                     LSGCGCDCEADCDCESNHANNSDCSDCECN"
     gene            986..2065
                     /gene="aroC"
                     /locus_tag="PLR72_06195"
     CDS             986..2065
                     /gene="aroC"
                     /locus_tag="PLR72_06195"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOK36825.1"
                     /translation="MANTIGKLFTFTSFGESHGPAIGGVIDGCPAGLKIDENFIQNEL
                     NRRRPGQSDITTSRKEEDKVVFLSGIFEGVTTGTPIAFVIWNTNQQSADYKHLKEIYR
                     PSHADFTYQQKYGIRDYRGGGRSSARETATRIVAGAIAKLVLNKLGINIVAFTSQVGP
                     IAMTKTYEAVDLSLIETNAIRCPEPEIAEKMIAYIQELKKEGDSVGGIITCVIKGVPV
                     GWGEPIYDKLQARLAQAMLSINATHGFDYGKGFDGVVMKGSEMNDEFIRVGDKITTKT
                     NYSGGIQGGISNGQDIYFRVLFKPVATISKKQTTVNIHSEEVELEARGRHDPCVLPRA
                     VPIVEAMAAITLLDMYLIKSRRIEE"
     gene            complement(2040..2777)
                     /locus_tag="PLR72_06200"
     CDS             complement(2040..2777)
                     /locus_tag="PLR72_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOK36826.1"
                     /translation="MKALILAAGQGTRLKPLTNSIPKALITINGRTLLEYQILKLKNY
                     GFDEIIINVHHFPDQIIDFLKSKNNFGLNIKISDERDFLLDTGGGIRQTEWFFNDGKP
                     FLVHNVDIFSNIDLGQLYQQHLSLNGLATLVVSPRKTSRYLLFDENLQLKGWFDEKTQ
                     EIKPTTININQFPKLAFAGIQILSPQVFKLMKNWEPPFSIIDFYLQNMEQHPIFGYIP
                     PKFKILDVGKIQALGEAEKFIPQFFDF"
     gene            complement(2829..4256)
                     /locus_tag="PLR72_06205"
     CDS             complement(2829..4256)
                     /locus_tag="PLR72_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOK36827.1"
                     /translation="MNELENLFVKYTGEKLLSKEELSASGSHRRYFRLESKNNSLIGV
                     EGTSKEENRAFIELAKHFYQQGLPVPLFLTQSSDGMYYLEEDLGNTLLFDYIAEGRKT
                     GVFCESEKEMLRKTIRELPKIQVKGAINLDFSICYPQPEFNERSILWDLNYFKYCFLK
                     ATGLDFQENLLEDDFKKLSNILLKNQTNTFMYRDFQSRNVMIKNNEPYFIDFQGGRKG
                     PVYYDVASFLWQAKANYNNELREELLQNYLEVLASLISFNESEFREQLKHYILFRTLQ
                     VLGAYGFRGYFEKKPHFLQSIPFAIENLRSILQDNITGYSYLIKVLQQLTELKQFREV
                     SVRKPLTVKIYSFAYKKGIPQDDSGNGGGFVFDCRAINNPGKYEKFTYFSGLDEAVKQ
                     FLEEDGEILTLLENAYNLVDASVKRYMDRGFTNLMVSFGCTGGQHRSVYAAQKMAEHI
                     HQKFGVEVHLIHRELNKEELFLATA"
     gene            4551..>4926
                     /locus_tag="PLR72_06210"
     CDS             4551..>4926
                     /locus_tag="PLR72_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HOK36828.1"
                     /translation="MKTKNGLMILFLSFAIILSGCKSLNKTAKGTMIGAGTGAAVGAG
                     IGAIVGKDAKSAGIGAAIGTAVGATTGAIIGHKMDKAAEKAAAIEGAKVETITDINGL
                     PAVKVTFESGILFETNKSTLNEA"
BASE COUNT         1697 a          712 c          871 g         1646 t
ORIGIN      
        1 cacttcttat gattttgttg caaaacaatt tacttataca aatgtaagtg tttcaagaga
       61 tttacattgt tggaatattt ctgctaattt tgttcctttc ggacctttcc agtcttatta
      121 ctttagaatt ggcgtaaatt cgagcatgct tcgtgacctg aaatatgaaa aacgtagtgg
      181 ttatggttca acacccattg attggtattg agttataact gaaaaataaa gagattttac
      241 ttttaaaatg aacaatttat tttaatagtc gttataaaaa aataattaat cttgatttat
      301 aaatttaaaa attagaaaat gaaaattgat gtaaacaaag tagtgtctgc aaaatatgaa
      361 ttatttgtag aaggagaaaa tgaaggtgaa ttgaaattaa tggaaagagc cactgaacaa
      421 gaacctttaa gttttattta tggtgtagga atgatcttgc ctaaatttga agaatatttg
      481 tatggtttgg aagcaggaga tgattttgaa tttactattg atagcgagga tggctatggt
      541 cattatgata aggaagatat aatagacctt gatcgaagta tttttgaaac agatggcaaa
      601 ttagataata acgtcatttt tgaaggaaat gtagttacct tgatggataa tgaaggtaat
      661 cgtttacctg ctaaaattgt aacaatcact gaaaattatg tgacggttga tttgaatcat
      721 ccactggcag atgagaaact tcatttcaaa ggaactgttt tggaagtgag agatgctacg
      781 gaagaggaac tttcttcact ttctggatgc ggttgcgatt gtgaagcaga ttgtgattgc
      841 gaaagcaatc atgctaataa tagcgattgt agtgattgtg aatgtaatta gttttatatc
      901 aataaattac taaaagcata atttttgcct ttttaatgag aatttatttt tttttgtaaa
      961 gcgagataaa aaagtttaat ttcttatggc aaatacaatt ggtaagttat ttacttttac
     1021 ctcttttggt gaatctcatg gacctgctat cggaggcgtt attgatggtt gtcctgctgg
     1081 gctcaaaata gatgaaaatt ttattcaaaa tgaattaaat cgacgtcgtc ccggtcaatc
     1141 tgatattact acttcccgaa aggaagaaga caaagtagtt tttttgtcag gtattttcga
     1201 aggagtcaca acgggaacgc ctattgcttt tgttatctgg aatacaaatc aacagagtgc
     1261 agattataag catttaaaag aaatttatcg tccttctcat gccgatttta cttatcagca
     1321 aaaatacggt attcgcgatt atcgaggtgg tggtcggagt tcagcacgcg aaacagcaac
     1381 cagaattgtt gctggtgcca ttgcaaagtt agttttaaat aaattaggaa ttaatattgt
     1441 ggcttttact tcacaagtgg gacctattgc aatgaccaaa acctatgaag cggtagattt
     1501 gtcgcttatt gaaaccaatg cgattcgatg cccagaacct gaaattgccg aaaaaatgat
     1561 tgcctatatt caagaattga aaaaagaagg tgattctgtt ggtggaatta ttacctgtgt
     1621 gataaaaggt gttcctgtcg gatggggaga acccatttac gataaattac aagctcgtct
     1681 tgctcaagca atgctcagca tcaatgctac gcacggattt gattatggaa aaggttttga
     1741 tggtgttgtg atgaaaggtt ctgaaatgaa tgatgaattt attagagtgg gagataaaat
     1801 tactaccaaa acgaattatt ctggaggtat tcagggaggc attagtaatg gacaagatat
     1861 ttatttcaga gttttgttta agccagttgc tacaatttca aaaaaacaaa ctaccgtcaa
     1921 tattcattct gaggaagttg agctggaagc tcgagggcgt catgatcctt gtgttttgcc
     1981 tcgagcagtt cccattgtgg aagcaatggc tgctattact ctgctggata tgtatttgat
     2041 taaaagtcga agaattgagg aataaacttt tccgcttcgc cgagagcttg aattttcccc
     2101 acatccaata ttttaaattt aggaggaatg tatccaaaaa tcggatgttg ttccatgttt
     2161 tgtaaataaa aatcaataat tgaaaatggt ggttcccaat ttttcatcag tttaaaaact
     2221 tgtggagaaa gaatttgtat tccagcaaaa gctaattttg ggaattgatt aatgtttata
     2281 gtcgttggtt taatttcttg tgttttttca tcgaaccagc cttttagttg tagattttca
     2341 tcaaataaaa ggtagcgtga ggttttacgc ggacttacta ctaacgttgc caatccgtta
     2401 agcgaaagat gttgttgata aagttgccct aaatcaatat tagaaaaaat atctacatta
     2461 tgaaccaaaa atggtttgcc atcattgaaa aaccattccg tttgacgaat gccaccacct
     2521 gtatctaata aaaaatctct ttcgtcagaa attttaatat tcaatccaaa attatttttt
     2581 gattttaaaa aatcaataat ttggtctggg aaatggtgaa cattaataat gatttcgtca
     2641 aaaccataat ttttcaattt taaaatttga tattccagta aagttcttcc attaatagtg
     2701 attaacgctt ttggtataga attagttaat ggttttaaac gggttccctg tccagcagca
     2761 agtattaaag ccttcattgt ttaaaaacga tggtattttt tgaaattctt aatttttagt
     2821 cagaaatttt aagcagttgc aagaaataat tcttctttat ttaattctct atgaataaga
     2881 tggacttcaa caccaaattt ttgatgaata tgttcagcca ttttttgagc agcgtagacg
     2941 gaacgatgtt gaccaccagt acatccaaaa ctaaccatta aattggtaaa gccacggtcc
     3001 atataacgtt ttacagaagc atccactaaa ttataagcat tctccaaaag agtaaggatt
     3061 tcgccatcct cttccaaaaa ttgttttaca gcctcatcaa gtcctgaaaa ataagtaaat
     3121 ttttcatatt tgccgggatt attaatagct ctacaatcaa aaacaaatcc accaccattt
     3181 ccagaatcat cttgtggaat acctttttta taagcaaaac tataaatttt tactgttaat
     3241 ggttttcgaa ctgaaacttc gcgaaactgc ttcagttctg taagttgttg taaaactttt
     3301 atcaaataag aatagcctgt aatattatct tgtaaaatag atcttaaatt ttcaatagca
     3361 aagggtatgc tttgtaagaa atgaggtttt ttctcaaaat accctctaaa tccataagct
     3421 cctaaaactt gtaaagtgcg aaaaagtata taatgtttca attgttccct aaactctgat
     3481 tcattgaaag aaataagaga agcaagcacc tctaaataat tttgtaaaag ttcttcgcgg
     3541 agttcattat tgtaattggc tttagcctgc cagagaaaag aagcaacatc atagtaaaca
     3601 ggaccttttc ttccaccctg aaaatcaata aaataaggct cattattttt aatcattaca
     3661 tttctgctct gaaaatcacg atacataaac gtattggttt gattttttag caaaatattg
     3721 gaaagttttt taaaatcatc ttctaacaaa ttttcctgaa agtcgagccc agtagctttc
     3781 aaaaaacaat atttaaaata attcagatcc cataatatcg aacgttcatt aaattctggt
     3841 tgagggtaac aaatagaaaa atctaagttg atagctcctt taacttgaat ttttggcaat
     3901 tctcgaatgg tttttcttaa catttctttt tctgattcac aaaaaacgcc tgtttttctc
     3961 ccttcggcta tataatcaaa aagcaaagta ttccctaaat cttcttccag ataatacata
     4021 ccgtctgagg attgagttaa aaagagtgga acaggcaaac cttgttgata aaaatgtttt
     4081 gctaattcaa tgaaagctct gttttcctct tttgaagtac cttccacccc aattaatgag
     4141 ttatttttgc tttctaagcg aaaataacgt cgatgagaac ccgaagcgga aagttcttct
     4201 tttgataata atttttctcc ggtatatttt acaaataaat tttctaattc gttcatttta
     4261 aataatgcta tgccttaatt ataattcaaa gataaataaa tagtttttat agtcaaaaag
     4321 agaaattaaa ctgatgaata taaagttaaa aatacttttt caaatttaac aaattacgtt
     4381 tgaaaattaa ataaaaacaa attatctttg aaaaaaatta tgggggaatt atttttatac
     4441 attgttttaa acaaaaaaaa tttattcgtc aataaaatag cgtttttcaa aaaaattgaa
     4501 aaagaaacta ttagtaaatt atgttttatt aactttaaaa atttatgaat atgaaaacaa
     4561 aaaatggatt aatgattttg ttcctatcat ttgctataat tttaagtggc tgtaaatccc
     4621 taaacaagac agcaaaaggt accatgattg gtgcagggac gggcgctgcc gtcggggcag
     4681 gaataggagc tattgtaggt aaagatgcga aaagtgctgg aataggtgct gctattggaa
     4741 cagcagttgg agcaacaaca ggtgcaatta ttggacacaa aatggataag gcagcagaaa
     4801 aagcggctgc aattgaagga gcaaaagtag aaaccattac tgatattaat gggcttccag
     4861 cagtgaaagt aacttttgaa tcaggtattt tatttgaaac gaataaaagt actttaaatg
     4921 aagcgt
//
LOCUS       DAOSAS010000125         4819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_11109_length_4819_cov_14.420655, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000125 DAOSAS010000000
VERSION     DAOSAS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11109_length_4819_cov_14.420655"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLR72_06215"
     CDS             complement(<1..509)
                     /locus_tag="PLR72_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOK36829.1"
                     /translation="MKRLFCLTFFFVSVFGATYSQEEMIPRGGARLQVKIEGNDTIFL
                     AYLPDLWVFPGFKDRKQEQYYWKLVRDVKRTLPYAKIVAKELSETNKLLMQMPNDKER
                     KKYLAQYEKDIYKKYEPELRKLTLSQGKLLIKLIDRECNNTSYDLIKMYRGSIRAFFY
                     QGFARIFGA"
     gene            643..1461
                     /locus_tag="PLR72_06220"
     CDS             643..1461
                     /locus_tag="PLR72_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36830.1"
                     /translation="MKKHFCLFFVLSFSSLIFSQISYIVPSSASIANTSVSNTHEWTA
                     FQNPAMLGYGENSEIGLQYENRFLLSQISTSSFQALIATPVVNSDISLSYFGYSFYHE
                     IIGAVGFGRNFSNKFSLGLRFNYYAAYFSASNSYRGILFPHLGFSYRFTPNFSIGCSA
                     FNPFQMNIKTETAEKQIPSVFSIGSEYYFTPELIWLTQLDKEISSNYRIATGFEYQML
                     KAISVKIGAYTAGYLVPCLGMGLNFGNLKLDFNAELHPLLGLNSFVALRHSFRK"
     gene            1466..3535
                     /locus_tag="PLR72_06225"
     CDS             1466..3535
                     /locus_tag="PLR72_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOK36831.1"
                     /translation="MKRFRFYMVIILLAVTQIKGKAQDALSTIEQTIADIFEQYQQEV
                     EEELDFNNFLEDLLFVAENPIDLNRATREELEKLPFLSDIQIENILYYLYQFGPMQTI
                     YELQLIDGLDMTDIRRMLPFVVVHPLKKENPKLYWHDLLNYGKNDLYIRLDRKLETKR
                     GYLSISPEKNSDVSSKPYLGDPFYNSIKYRYHFRDKIYFGFTTEKDPGEPFLDKIQTN
                     YDFLSGYLQLNNIGKFKTIILGDYRANFGQGLVLRQEFSMGKSSYVMNVIPRGSGLKK
                     YSSTDEYNFFRGLGTTLQLGNTEISAFYSNKQIDGDTIQGCFSSIYKTGYHRTSNEFK
                     KKKTVNQQVVGGNLTYYFPHLQIGATVVHTLFNHFLEKEETTYNHFYFSGNQQTTGGI
                     NYRFRWKKLNFFGETALSNHFSWATLNGCFFSPFSLLDIVVLYRNYSPDYDTFYANAF
                     SESTRINNEKGMYLGAELRPIKNWKFSAYADSYRFSWVKYRVDAPSFGNDYLLQVDFS
                     PSRMVNMFWRLRYEEKQENLSQDTSIMSFVVPLRKASLRYQLNYVAGNFSFRTVLEGN
                     LAQKSEEASWTYGLTAYQDLSYEFRSFPLRLDFRYQFFDATSYENRVYAYEKDVLYAF
                     SIPMNYGLGSRYYLNLRYKFNHQLSCWLKFAQTVYADGRTTIGSGYELIQGNRKSDIR
                     FLLHYKF"
     gene            complement(3539..4741)
                     /locus_tag="PLR72_06230"
     CDS             complement(3539..4741)
                     /locus_tag="PLR72_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOK36832.1"
                     /translation="MGKVLIIGAGGVGTVVTHKVAQNPEVFTDIMLASRTKSKCDAIA
                     TEVKNRYGVEIKTASVDAENVPELVKLLKGFQPELVINVALPYQDLTIMDACLEAGVN
                     YLDTANYEPKNEAHFEYSWQWAYKDRFEKAGLTAILGCGFDPGVTSVFTAYAAKHHFD
                     EIQYLDIVDCNAGDHGKPFATNFNPEINIREVTQRGKYWENGQWVETEPHEIHKPLTY
                     PEIGPKESYLIYHEELESLVKNFPTIKRARFWMTFGEEYLTHLRVIQDIGMARIDEVE
                     FNGQKIVPLQFLKAVLPNPGDLGENYKGWTSIGCRIRGIKNGKEKTYYIYNNCSHEAA
                     YKETGTQGVSYTTGVPAMIGAMMFLQGKWKKPGVFNVEEFDPDPFMEQLNKQGLPWHE
                     LVNVDLEL"
BASE COUNT         1453 a          849 c          846 g         1671 t
ORIGIN      
        1 ttggctccaa agatgcgggc aaatccttga taaaaaaagg cacgaatgct tcctctatac
       61 attttgatta aatcgtagga agtattgttg cactctctat caattagttt aattaataat
      121 tttccttgac tgagggttaa ttttcgcaac tcaggttcat attttttata aatatccttt
      181 tcgtattgtg ccaaatattt tttccgttct ttgtcatttg gcatctgcat tagtaattta
      241 ttggtttctg ataactcttt tgccacaatt ttagcgtatg gcaaagttcg ttttacatcg
      301 cgaaccaact tccaataata ttgttcctgt tttctatctt taaaaccagg gaatacccat
      361 aaatcgggaa gataagccag aaagatagtg tcatttcctt caattttgac ttgaagacgc
      421 gctccaccac gaggaatcat ttcttcctga gaataagtag ctccaaatac agagacaaaa
      481 aagaaagtca gacaaaataa tcgtttcata attgcaaatg tacgtaaaaa taaaagccaa
      541 gaaaaaagct caaattaaga attattattg aagaatatta tattttttat acttttgaat
      601 tttacttaaa ttttttgagt atttgattac aaatcatatg ttatgaaaaa acacttctgt
      661 ttgttttttg ttctatcatt tagctcatta attttttcac aaatttctta tatagttcct
      721 tcttctgcgt ccattgcaaa tacttccgtt tcaaacactc acgagtggac ggcgttccaa
      781 aatcctgcta tgttgggtta tggtgaaaat tcagaaattg gacttcagta cgaaaatcgt
      841 tttctccttt cgcaaatttc tacgagctct tttcaagctt tgattgctac acctgttgta
      901 aatagtgata tttcgctctc ctattttggt tattcatttt atcatgaaat tatcggcgct
      961 gttggttttg gtagaaattt ttccaataaa ttttcattag gacttcgttt caattattat
     1021 gccgcttatt tttctgcttc caactcttac cgcggaattt tatttcctca tcttggcttt
     1081 tcttatcgct ttacacccaa tttcagtatt ggatgcagtg cgtttaatcc ttttcaaatg
     1141 aatatcaaaa cagaaactgc tgaaaagcaa attccttctg tttttagtat tggaagtgaa
     1201 tattatttta ctccagaatt gatttggctg acacaactgg ataaagaaat cagtagtaat
     1261 taccgaatag caacgggatt tgaatatcaa atgctaaaag ccatttcggt taaaataggt
     1321 gcttatacgg ctggttatct tgtcccttgt ctgggaatgg gactgaattt tggaaatctt
     1381 aagctcgatt ttaatgctga attacaccca ctcttaggtt taaattcatt tgttgcctta
     1441 cgtcacagct tcaggaaata aatttatgaa acgattcaga ttttacatgg taattatttt
     1501 attggctgtg acgcaaataa aaggaaaagc acaagatgct ttgtcgacca ttgaacaaac
     1561 gatagcggat attttcgaac aatatcagca ggaagtagaa gaagaattgg atttcaacaa
     1621 ttttttagaa gatttattat tcgtggctga aaatccaatc gatttaaatc gtgctacacg
     1681 agaagaattg gaaaaattac cttttctatc ggacattcaa attgaaaata ttctttatta
     1741 tctgtatcag tttggtccta tgcaaaccat ttatgaacta caactcattg acggtctgga
     1801 tatgacagac attagacgaa tgttgccttt tgtagtagtt catccactca agaaggaaaa
     1861 tccgaaatta tactggcacg atcttttgaa ttatggaaaa aatgatttat acatccgtct
     1921 ggatagaaag ctcgagacca aaagaggata cctatcaatt tcaccagaaa aaaattccga
     1981 tgttagttca aagccatatt taggagatcc attttataat tcaataaaat atcgttatca
     2041 ttttcgggat aaaatttatt ttggttttac cactgaaaaa gacccaggag aaccattttt
     2101 agataaaatt caaacaaatt atgattttct ttcaggttat ttacaattga ataatattgg
     2161 caaatttaaa accattattt tgggcgatta tagagccaat tttggtcaag gtcttgtttt
     2221 gcgtcaagag tttagtatgg ggaaatcatc ttacgttatg aatgtaattc cacgtggatc
     2281 aggattaaaa aaatatagtt ctactgatga atataatttt tttcgcggat tgggcacaac
     2341 tttacaattg gggaacacag aaatttcggc attttattcc aataaacaaa tagatggcga
     2401 caccattcaa ggttgttttt caagtattta caaaacaggt tatcatagaa caagtaatga
     2461 attcaagaag aaaaaaacgg ttaatcaaca ggttgtagga gggaatttga cttattattt
     2521 tccgcattta caaattggtg caacagtagt tcatactttg ttcaatcatt ttttagaaaa
     2581 agaagagacc acctataatc atttttattt ttctgggaat caacaaacta cgggcggaat
     2641 aaattaccgt ttccggtgga aaaaattaaa tttttttgga gaaactgctc ttagcaatca
     2701 tttttcatgg gcaactttaa atggctgttt cttttctcct ttttctttgc tggatatagt
     2761 ggttttatat cgcaattatt ctcctgatta tgacacgttt tatgccaatg ctttttcgga
     2821 atctacgcga atcaataatg agaaaggaat gtatttggga gccgaattac gtccgataaa
     2881 aaattggaaa ttttctgcct atgcagatag ttatcgtttc tcgtgggtta aatatcgggt
     2941 tgatgcacct tcgtttggaa atgattattt gcttcaggtt gattttagtc cttctcgcat
     3001 ggttaacatg ttttggagat tgagatatga agaaaaacaa gaaaatttat ctcaagatac
     3061 ttcaataatg tcttttgttg tacctttaag gaaagcctcc ttacgttatc agctaaatta
     3121 tgttgctgga aattttagct ttcgtactgt tctggaaggc aatcttgctc aaaaaagtga
     3181 agaagcaagt tggacttatg gtttaacggc ttatcaggat ttgagttatg aatttcgttc
     3241 ttttccttta agacttgatt ttaggtatca gttttttgat gcaacttctt acgaaaatcg
     3301 tgtttatgct tatgaaaaag atgtgttgta tgctttttcc attcctatga attatggttt
     3361 aggaagcaga tactatttga acttaaggta taagttcaat catcaacttt cttgctggtt
     3421 aaagtttgct caaactgttt atgcagatgg aagaactact attggcagtg gatatgagct
     3481 cattcaagga aatcgaaaat cagacattcg atttttatta cattacaaat tttaaaaatt
     3541 acaattccaa atcaacattc accaattcat gccagggtaa accttgctta ttgagttgct
     3601 ccatgaaagg atctggatca aattcttcta cattgaaaac acccggcttt ttccattttc
     3661 cctgcaaaaa catcatggct ccaatcatgg caggaacgcc ggtggtataa ctcacgccct
     3721 gtgtaccagt ttccttgtaa gctgcttcgt ggctgcaatt gttgtagata tagtaagttt
     3781 tttccttgcc gttttttatg ccacgaatgc ggcaaccaat agaagtccaa cccttgtagt
     3841 tttcacctaa atcgcctgga ttcggcaata cggcttttaa aaattgaaga ggtacaattt
     3901 tttgtccgtt aaattcaact tcgtcaattc gagccatgcc aatatcctga atgacacgta
     3961 aatgtgttaa atattcctct ccgaaagtca tccagaaacg tgctcgcttg atggttggaa
     4021 aatttttcac cagcgattcc agctcttcat gataaatcag atacgattct ttcggtccga
     4081 tttccggata agttagtggt ttatgaattt cgtgcggttc tgtttccacc cactgaccat
     4141 tttcccagta ttttccgcgt tgcgtaactt cgcgaatatt gatttcggga ttaaaatttg
     4201 tggcaaaagg tttaccgtga tcgccggcat tgcaatccac aatatcgaga tattgaattt
     4261 cgtcaaaatg atgtttggca gcataagccg taaaaacgct cgtaacgccc ggatcgaatc
     4321 cgcagcccag aatggctgtc aatccggctt tttcgaaacg atctttatat gcccattgcc
     4381 agctatattc aaaatgggct tcgtttttcg gttcgtaatt ggctgtgtcc agataattta
     4441 ctccggcttc cagacaggca tccataatgg tcaaatcctg atagggaagc gctacattga
     4501 tcacaagttc cggttgaaat cctttaagca gttttaccaa ttcgggaaca ttttcagcat
     4561 caaccgaagc tgttttaatt tctacaccat aacgattttt tacctctgtt gctatggcat
     4621 cgcatttcga tttggtacgg cttgccagca taatgtcggt gaaaacttcc gggttctgtg
     4681 caaccttgtg cgtaacaacc gttcccactc ctccggcgcc gataatcaat actttcccca
     4741 tttttctttt ttgtgttgaa aattaataag ttaaaaatat tagcctgcaa aagtacattt
     4801 ttttaaggaa acgaaaaag
//
LOCUS       DAOSAS010000126         4760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_11296_length_4760_cov_13.303932, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000126 DAOSAS010000000
VERSION     DAOSAS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11296_length_4760_cov_13.303932"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..391)
                     /locus_tag="PLR72_06235"
     CDS             complement(89..391)
                     /locus_tag="PLR72_06235"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOK36833.1"
                     /translation="MKFIKKIFKTFKKIILNKYILVFLLFMVYFIFFDKHNLISRWQI
                     YQKNKQLEKELNYYKKKIEEDKLKLYELKSSDENLEKFARERYYFKKDKEDIFVLP"
     gene            complement(478..1935)
                     /locus_tag="PLR72_06240"
     CDS             complement(478..1935)
                     /locus_tag="PLR72_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018967644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOK36834.1"
                     /translation="MKSKLLTLFCLFFSLIIWAVPPAGYYTSSTLDGKNTNNLQIAFQ
                     AIITNGHSVTSYDGLWNAYKTTDINPSTGKIWDIYSNNCNFTWGNDQCGNYSKECDCY
                     NREHTTPQSWFNGASPMYSDLFNVYPTDGYVNNKRSNYPYGEVGSATYTSGNGSKLGT
                     SNFSGYNGTVFEPIDEYKGDLARTYFYMATRYADQCGTWTNGNGSVVYTSTLGLTFYA
                     INLFLKWSREDPVSQKEIDRNDAVYSIQNNRNPFIDFPGLEEYIWGNKTSEPFYVNNT
                     TVVKPTVNSPVVSNITAYSATLGGTVISDGGGTITETGIYYSTTNGFADGDGIKISTT
                     TPVSGAFTVSVVNLIPSTTYYFKAYATNSAGTGFSEQASFTTKNPSTDIQSEIILGNP
                     VPSGATFDYGKVSNNATKSFFIKTKNLIGDLSVIVSGEMFSVSTNIISKTDADAGYTL
                     YIYYQPTESGFHSGSVTISGGGLPADFIIRLTGSR"
     gene            2104..2544
                     /locus_tag="PLR72_06245"
     CDS             2104..2544
                     /locus_tag="PLR72_06245"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOK36835.1"
                     /translation="MKENQDIFFMRQALVEAKEAAKKNEVPIGAVIVCGGKIIARAHN
                     LTETLIDVTAHAEMQAITSAANYLGSKYLMDCTLYVTVEPCVMCAGALGWSQIGRIVY
                     GTSDEKRGYTIFSPQALHPKTEVTSGILSEECKNLIQEFFRKKR"
     gene            2816..4333
                     /gene="guaA"
                     /locus_tag="PLR72_06250"
     CDS             2816..4333
                     /gene="guaA"
                     /locus_tag="PLR72_06250"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOK36836.1"
                     /translation="MEKIVILDFGSQTTQLIARRLRELNVFSEIIPYDKFPKNTKDIK
                     GIILSGSPFSVYEPKAFKIDLHTLPQDIPILGICYGAQLIVESYKGKVEATHNCEYGR
                     AVLSYIDTNDPLFRNFPSGSQVWLSHSDSITELPKDFIKIASTPDLEITAYRVKGKKI
                     WGVQFHPEVHHTVNGMLLFENFLDICGCKRDWTPTSFVESTIEQLKKQLNNDKVILGL
                     SGGVDSSVTAMLLSKAIGKNLTCIFVDHGLLRKNEFEEVLSEYQHLGLNVIGVDVKDR
                     FYSALKGITDPEQKRKIIGAKFIEVFNEEAKKIEGVKWLAQGTIYPDVIESMSPSGNT
                     IKSHHNVGGLPEKMDLKLCEPLRLLFKDEVRKVGIELGMMHQFIKRHPFPGPGLAVRI
                     LGEVIPEKVQILQEADDIFIKNLKEWNLYDKVWQAGAILLPIQSVGVMGDERTYENVV
                     ALRAVTSTDAMTADWAQLPYEFLSKVSNDIINKVKGINRVVYDISSKPPATIEWE"
     gene            4348..>4760
                     /locus_tag="PLR72_06255"
     CDS             4348..>4760
                     /locus_tag="PLR72_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOK36837.1"
                     /translation="MIDTHSHIYLEEFDEDRKEVIQRAKDIGVQYIILPNIDSSTLPR
                     VHQVEKEERDYCFAAIGLHPTSVKENFKEELEVIKSELQQRKYIAIGEIGIDLYWDKK
                     FYKEQEEAFCQQVEWAIKYELPILVHIRNSFEETI"
BASE COUNT         1663 a          727 c          801 g         1569 t
ORIGIN      
        1 ttttttacaa acaaaataat gaccgtgatt ttctgcattt aataaaatat tattttcgtt
       61 tagtcgtcat gtttgttctt aaacaattct atggtaaaac aaagatgtct tccttatctt
      121 ttttgaaata ataccgctca cgagcaaatt tttctaaatt ttcatcactc gattttaatt
      181 catacaattt caacttgtct tcctctatct tttttttata ataatttaat tccttttcta
      241 actgcttatt tttttgataa atttgccatc gggaaattaa attatgctta tcaaaaaaga
      301 taaaatatac cataaaaagt aaaaaaacaa ggatatattt attcaatata atctttttga
      361 acgttttaaa aattttcttg ataaatttca tccttacctt actttttaaa tttatgcttg
      421 aactattttt ttactattga gttaacaaat ataatatgct cgcatttttt aataaagtta
      481 ccttgaacct gtcagtcgaa taataaaatc agcaggcaat cctccaccag aaattgttac
      541 gcttcctgag tgaaaaccgc tttcggtcgg ctgataatat atgtaaagtg tgtaacctgc
      601 atcagcatct gttttggaaa taatatttgt agaaacacta aacatttcac cagaaacaat
      661 tacagaaaga tctcctataa gattttttgt cttgataaaa aatgatttag tagcattatt
      721 actcacttta ccataatcaa atgttgctcc tgaaggcaca ggattcccta aaataatttc
      781 tgattgaata tcggtagaag gatttttagt ggtaaaactt gcttgttctg aaaaacctgt
      841 ccctgcacta tttgtagcat aagctttaaa ataatacgta gtagaaggta taagattaac
      901 aaccgagact gtaaatgcac cacttactgg agttgtagtg ctaattttta ttccatcgcc
      961 atcagcaaaa ccgttcgttg tactataata tataccggtt tcagtaattg tccctccacc
     1021 atcagaaata actgtgcctc ccaaagtagc actataagca gtaatatttg ataccacagg
     1081 cgaatttaca gttggtttga ccacagtagt gttatttaca taaaaaggtt cagatgtttt
     1141 atttccccaa atatattctt ccaacccagg aaaatcaata aaaggatttc tgttgttttg
     1201 aatactatac accgcatcat tgcgatcaat ttctttttgg ctgacaggat cttcacgact
     1261 ccacttcaaa aataagttga tagcataaaa agtaagtcca agagtagaag tatagacgac
     1321 tgaaccattc ccatttgtcc atgttccgca ttgatcggca taacgagtag ccatgtaaaa
     1381 ataagtacgg gctaaatcgc ctttatattc atcaatcggt tcaaaaacag ttccattata
     1441 acctgaaaaa ttggaagtac ccaatttgct cccattacca gaagtataag tagcagatcc
     1501 gacttctcca taaggataat tgcttctttt attgtttacg taaccgtcgg taggataaac
     1561 attgaataaa tcagaataca tgggagaagc accattaaac caactttgtg gagtagtatg
     1621 ttcacgattg taacaatcac attccttact gtagtttcca cactgatcat taccccaagt
     1681 aaagttacaa ttattggaat atatatccca aattttacct gttgaaggat ttatatcagt
     1741 tgttttgtat gcattccata agccatcata agatgtcaca ctatgtccat tggtgataat
     1801 agcctgaaaa gctatttgca aattgtttgt atttttccca tccaatgtag atgaagtata
     1861 ataacctgct ggtggaacag cccaaatgat gagagagaaa aataaacaaa atagcgttaa
     1921 caactttgat ttcataaaat tttttattct taaaaagagt cttaaaatta attttaaaaa
     1981 acaaagttat aacttttctg aaacaataaa ataaaatcac aaaataattg tttcagttaa
     2041 aaatgatttt aattttgata attaaatttc gtttgtaaat tcaatttctt tttaatttta
     2101 aaaatgaaag aaaatcagga tatatttttt atgcgacagg cactggtaga agcaaaagaa
     2161 gctgcgaaaa aaaatgaggt tcccattggt gcagtcattg tgtgtggagg aaaaatcatt
     2221 gcacgagctc ataatttgac agaaacattg atagatgtta ctgctcatgc agaaatgcaa
     2281 gcaattactt cggcagctaa ttatttaggg agtaaatatt taatggactg cacattatat
     2341 gtaacggttg aaccgtgtgt aatgtgtgct ggggcactgg gctggtcaca gattggaaga
     2401 attgtgtatg gaacttctga tgaaaaacga ggttatacca ttttttcacc tcaggcttta
     2461 caccccaaaa ccgaagtcac atcgggaata ttatcagaag aatgtaaaaa tttgatacaa
     2521 gaatttttca ggaaaaaaag ataaaaaaga catttatttt aaaagttcat aataggtagc
     2581 aaaaaatcta ccagcacagg tttatttcta tttataattt cttgaaatgt ttccatatct
     2641 ccttcttttt tgttaagaaa ggatggtctt gggtttttct ttctacttgt tctaaaattg
     2701 ttaaaaataa gtcactaata ataaaataaa aaactctcat tatttgtttg taaaaataca
     2761 acattgtttt tacttttgca taacttttat caaagtttga atctattaaa aatatatgga
     2821 aaagattgtt attcttgatt ttggttctca aactactcaa ttaatagctc gtcgtctgcg
     2881 tgagttgaac gtttttagtg aaataattcc ttatgataaa tttcctaaaa atacaaaaga
     2941 tataaaaggt attattttat caggaagtcc cttctcagtg tatgagccga aagcatttaa
     3001 gattgatttg cataccttac cccaagacat tcctatttta ggaatttgtt atggagctca
     3061 acttattgta gaaagttata aaggaaaagt agaagctacc cataattgcg aatatggacg
     3121 agcagtgctt tcctacatag atacaaatga cccgttattt cgaaattttc cttcaggttc
     3181 acaagtctgg ctgtcacata gtgattctat tactgaatta ccaaaagatt ttataaaaat
     3241 tgccagtaca cctgatttag aaataacagc ttatcgggta aagggtaaaa aaatctgggg
     3301 tgttcaattt catcctgaag ttcatcatac tgtcaatgga atgttattat ttgaaaattt
     3361 tctggatatt tgtggatgta aacgtgactg gacacctaca tcctttgttg aatctacaat
     3421 tgaacaattg aaaaaacaac ttaacaatga caaggtaatt ttggggcttt caggcggtgt
     3481 tgattcatcc gtgactgcta tgttgttgag caaagctatt gggaaaaatc tgacttgcat
     3541 ttttgtggat cacggtttat tgagaaaaaa tgaatttgaa gaagttttgt cagaatatca
     3601 gcatttggga ttgaatgtta ttggagtaga tgtaaaggac aggttttatt ctgctttgaa
     3661 aggaattacc gatccagaac aaaaacgaaa aattatcgga gcaaaattca tagaagtttt
     3721 taatgaagag gcaaaaaaaa ttgaaggtgt taaatggctg gctcaaggca caatttatcc
     3781 agatgtaatt gaatcaatgt cgccctcagg aaataccatt aaatctcatc acaatgtggg
     3841 aggtttgcct gaaaaaatgg acttaaaact ttgtgaacct ttgcgattgc tttttaagga
     3901 cgaagtacgt aaagtaggaa ttgaattggg tatgatgcat caatttatca aacgacatcc
     3961 ttttcctggt cctggattag cagtaagaat attaggcgaa gtgataccag aaaaagttca
     4021 gatattgcaa gaggcagatg atatttttat caaaaattta aaagaatgga atttgtatga
     4081 taaagtgtgg caagcgggtg ccattctact tcccattcaa tcagttggcg taatgggcga
     4141 tgaaagaact tatgaaaatg tggtagcttt acgtgcagta acttctactg atgctatgac
     4201 agccgactgg gctcaattac cttacgaatt tctttctaaa gtatccaatg acattatcaa
     4261 caaggtaaaa ggaattaatc gagtagtgta cgatataagt tccaaacctc cagctaccat
     4321 agaatgggaa taatgaattt ttagaaattg attgatacac attcacatat ttatctcgaa
     4381 gaatttgatg aggatagaaa agaagtaatt caacgagcaa aagatattgg tgttcaatat
     4441 attattttgc ccaacatcga cagcagtact ttgccgcgtg tacatcaagt agaaaaagaa
     4501 gaacgtgatt attgttttgc agctattggc ttacatccta ccagtgtaaa agaaaatttt
     4561 aaggaagaat tggaagtaat aaaatcagaa ttgcagcaaa gaaaatatat tgctattggt
     4621 gaaataggaa ttgatttgta ttgggataaa aaattttata aagaacagga agaagctttt
     4681 tgtcagcaag ttgaatgggc tattaaatat gaattaccta ttttagtaca tataaggaat
     4741 tcatttgaag aaacaataaa
//
LOCUS       DAOSAS010000127         4663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_11612_length_4663_cov_9.698785, whole genome shotgun sequence.
ACCESSION   DAOSAS010000127 DAOSAS010000000
VERSION     DAOSAS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11612_length_4663_cov_9.698785"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..1612
                     /locus_tag="PLR72_06260"
     CDS             386..1612
                     /locus_tag="PLR72_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOK36838.1"
                     /translation="MKKIAFFILAFSCIFSLSAKRVMTGIEVLKEQNFKLLEGKRVGL
                     ITNPTGVDVNLKSTIDLLNEAPNVKLVALYGPEHGVRGNVYAGGTISNEIDEKTGLPV
                     YSLYGKTRKPTPEMLKGIDMLVYDIQDVGCRSYTYISTLGLAMEAAAENNIEFVVLDR
                     PNPLGGEKIEGVITEDPFISFVSQFKIPYIYGQTCGELALMLNEENFLKKKCKLTVVK
                     MKNWKRSMKWEDTGLEWIISSPHIPYKESSYFYPVTGILGELYYLSTGVGYTLPFQIV
                     AAPWIKADELATAMNQLHLPGFIFRPIYLTPFYSTFQGENCQGVQIHITDYKKATLSD
                     VQFYVIDKINKLYPDKNIFLHADSTRFSMFDKVCGTNQVRLKFQENISIEDILNFWNK
                     DKEQYYQLSKKYYLYK"
     gene            1609..4431
                     /locus_tag="PLR72_06265"
     CDS             1609..4431
                     /locus_tag="PLR72_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HOK36839.1"
                     /translation="MINDFLKEIKAYLPKDNVYTDDLRLLAWGTDASFYRLIPKIAIQ
                     TSNEWEISFLLQKAAKYNLPVTFRAAGTSLSGQSLSDSVLIIAGQHWQNFEVLNNGNA
                     IRLQPGIIGERVNEILKPYGKKFSPDPASIKSAMIGGILANNASGMSCGIRENSYQML
                     VSARIVFADGAILDTSDEQSKNNFIQTHADFLREIERIRDEIKNDEELYSFIKKKYEI
                     KNVTGLSLQPFVDFEDPFDIILHLMVGSEGTLGFVSEAVFKTTDNCPYSASGFAYFEN
                     MYLACEAVQKLKNLPVSAVEFFDRKAIASVEDKFSNIPELKRLPADGAALLIKIESDD
                     EEILHQRIDQVENLLSNAKVLFPVHFTDQPDEYNAYWDMRSGIFPTVGSTRPPGTTCL
                     IEDVCFRMENLADATDDLHQILLRNHYEDAVIYGHALAGNLHFIISQRFDNEENIHQY
                     ETMMNEVVNLVVNKYHGSLKAEHGTGRNIAPFVSKEWGEKAYGFMKRIKQLFDPQGIL
                     NPEVIFNEHPRCYVENIKPLPNTHPLIDKCIECGFCEVNCVSAGFTISSRQRIVVQRE
                     IARLKATKENPKRLKKLEKEFLFMGNQSCATDGLCATSCPVEINVGDYVHYLREEQLK
                     NKKVAQFLGKWTAEHFHLVANSLRSVLSLAYTAEKILGKRAMSVITRGLRVISGNTFP
                     FWSPALPKPQHKIIPPPISDNPLKVVYFPSCLNQMMSVSSNDPEQTPLIEKMISFLNK
                     AGYDVIFPKNMEKMCCGSIWESKGLLSIADQKSSELEKTLLEASENGRYPILCDQSPC
                     LFRMRQKMHQLSLYEPVEFIDKFLLDKLEFYPIDEPITIHATCSTTKMGLKPTLIKIA
                     KLCSKQVLIPEEVGCCAFAGDKGFMMPELNAHALRKLRPQIEKSNVKTGYSNSRTCEI
                     GLNTNTGIPYMSIVYLVDKCTKRKEL"
BASE COUNT         1555 a          730 c          860 g         1518 t
ORIGIN      
        1 gcacctttca ggtcttccat gatttttttc atccattctt cagtggaaac aggtgaaaaa
       61 tcagccaata agtttaattt tttttctgac atatttttta tttttcaaaa attattatcg
      121 actgcaaaat tactacttta agtttagtta ttaaatgttt ttggagagaa aaactaaaaa
      181 acgttttata aaattaagtt ggtaatcact tttttaaaaa ataatttttt agaaggtttt
      241 ttcttgatga ttaaaattca atttttttat ttacatttgc ttcaaaatgc aaacgtttgc
      301 accaatgttt tatttaaaaa ttcctttaag aggaaagaga gttaatataa atctataaat
      361 ttattcaaaa agatcgtatc atcaaatgaa aaaaattgca ttctttattc tggcattttc
      421 ttgtattttc agcttgtcag ccaaacgtgt aatgacagga attgaggtat taaaagaaca
      481 aaattttaaa ttactggaag ggaaacgagt aggtttaatt accaatccta cgggagtgga
      541 tgtcaattta aaatcaacca ttgaccttct caacgaagct ccaaatgtaa aattagttgc
      601 tttgtatggt ccagaacatg gtgttcgggg aaatgtatat gcaggcggaa caatcagcaa
      661 tgaaatagat gaaaaaacgg gccttcctgt ttattccttg tatggaaaaa cccgcaaacc
      721 aactccagaa atgttaaaag gaattgatat gctggtttat gatattcaag acgtcggttg
      781 tcgctcttat acttatatta gcacattggg actggctatg gaagctgctg ctgaaaataa
      841 tattgaattt gtagtgctgg atcgtcccaa tcctcttgga ggcgaaaaaa ttgaaggagt
      901 aatcactgaa gatcccttca tttcatttgt gagccaattt aaaattccat atatttacgg
      961 ccaaacttgt ggtgaacttg ctctgatgct gaatgaagaa aattttttga agaaaaaatg
     1021 taaactcacc gtcgttaaaa tgaaaaactg gaagcgttcg atgaaatggg aggatactgg
     1081 tcttgaatgg attatctctt ctcctcatat accctataaa gagtcatctt atttttatcc
     1141 agtgactggc attctgggag aattatatta tttatctact ggcgttggtt atactttacc
     1201 tttccagata gtagcagctc cctggataaa agctgatgaa ctggcaacag ccatgaatca
     1261 attacacttg cccggattta ttttcagacc gatttactta actccttttt atagcacttt
     1321 tcagggagaa aattgtcagg gagttcaaat tcatatcact gattataaaa aagctactct
     1381 ttctgacgtc cagttttatg tgatagataa aatcaataaa ctttatccgg ataaaaacat
     1441 ttttcttcat gctgattcta cccgattttc aatgttcgat aaagtttgtg gcaccaatca
     1501 agtccggttg aaatttcagg aaaacatttc cattgaagat atcttgaatt tctggaataa
     1561 ggataaagaa caatattatc agctttcaaa aaaatattat ttatacaaat gataaatgat
     1621 tttttaaaag aaataaaagc ttatctacca aaagataatg tttatacgga tgatttacgg
     1681 ctgctggcat ggggaacaga tgccagtttt taccgtttaa ttccaaaaat tgctattcaa
     1741 accagtaatg aatgggaaat aagttttctt ttgcaaaaag ctgcaaaata taatttgcca
     1801 gtaactttca gagctgctgg cacaagcctt tcgggtcaat ctctttctga ttctgtttta
     1861 attattgcag gacaacattg gcaaaatttt gaagtgctaa acaacggcaa tgctattcgg
     1921 ttacaaccgg gcatcatagg agaacgcgta aatgaaattc tgaaacctta tggaaaaaaa
     1981 ttctcacccg atccagcttc cataaaatca gccatgattg gaggcatcct tgctaacaat
     2041 gcttcaggaa tgagttgcgg gattcgtgaa aacagttatc aaatgctggt ttctgctcga
     2101 attgtttttg cagatggagc tattcttgat acaagcgatg aacaaagtaa aaataatttt
     2161 attcaaaccc acgctgattt tcttcgggaa attgagcgaa taagagatga aatcaaaaat
     2221 gatgaagaat tatattcctt catcaagaaa aaatatgaaa taaaaaatgt gacaggcctt
     2281 tcacttcaac cattcgttga ttttgaagat ccttttgata ttattttgca tttaatggtg
     2341 ggttcggaag gaacattggg ttttgtttca gaagctgttt tcaaaacaac tgataattgt
     2401 ccatacagtg ccagtggatt cgcttatttt gaaaatatgt atcttgcttg tgaagcagtt
     2461 caaaaattaa aaaatcttcc tgtcagtgca gtagaatttt ttgatagaaa agcaattgct
     2521 tcagttgaag ataaattcag caatattccg gaacttaaac gtttgccagc tgatggagca
     2581 gctttgttaa taaaaattga atcggatgac gaagaaattc ttcatcaaag aattgatcaa
     2641 gtggaaaatc ttttatcaaa cgctaaagtg ctttttccag tacattttac tgatcagcct
     2701 gatgaataca atgcttattg ggatatgcgt tctggcattt tcccaacagt aggcagtact
     2761 cgtccgcctg gaactacttg tttgattgaa gatgtttgtt tcagaatgga aaatctggca
     2821 gatgcaactg acgatttgca tcaaatattg ctcagaaatc attatgagga tgccgtcatt
     2881 tatggtcatg cactggcagg gaatcttcat tttattatca gtcaacgttt tgataacgaa
     2941 gaaaatattc atcagtatga aaccatgatg aatgaagtgg tgaatctggt agtaaacaaa
     3001 tatcatggtt ctttgaaagc ggagcatggc actggaagaa atatagctcc atttgtgagc
     3061 aaagaatggg gagaaaaagc ttatggtttt atgaaaagga tcaagcaact ttttgatcct
     3121 caaggaattt taaatcctga agttattttc aatgaacatc cacgttgcta tgttgaaaat
     3181 atcaaaccat tacccaacac gcatccgctc attgataaat gtatcgagtg tggtttttgt
     3241 gaagtgaatt gtgtcagtgc tggttttacg atctcttctc gtcaacggat tgttgttcaa
     3301 cgtgaaatag ctcgtttgaa agcaacaaaa gaaaatccga agcgacttaa aaaactggaa
     3361 aaagaatttc ttttcatggg gaatcaaagt tgtgctacag atggactttg tgctacttct
     3421 tgtccggtag aaatcaatgt aggcgattat gttcattact taagagaaga acagcttaag
     3481 aacaaaaaag ttgctcaatt tttaggaaaa tggacagcag aacattttca tttggtggca
     3541 aacagtttaa ggtcagtgtt atcattggct tacacggctg agaagatttt agggaagaga
     3601 gcaatgtcag ttattacgag aggattacgt gttataagtg gaaatacttt tcctttttgg
     3661 tcacctgctt tgccaaaacc tcaacataaa attattccac cacccatctc tgataatcca
     3721 ttgaaagtgg tttatttccc cagttgctta aatcagatga tgagcgtcag ttcaaatgat
     3781 cctgaacaaa ctccacttat tgaaaaaatg atttcatttc ttaacaaagc aggttatgat
     3841 gttatttttc caaaaaatat ggaaaaaatg tgttgtggca gtatctggga aagtaaaggt
     3901 ttgctttcta tagccgatca aaaatcttct gaattggaaa aaactcttct cgaagcttca
     3961 gaaaatggcc gttatcctat attgtgcgac caaagtcctt gcttattcag gatgcggcaa
     4021 aaaatgcatc aattgtcttt atatgaacca gttgaattca tagataaatt tttattggat
     4081 aaacttgagt tttatcctat tgatgaaccg attactattc atgccacttg ttctaccaca
     4141 aaaatgggat taaaaccaac attgattaaa attgcaaaat tgtgcagcaa gcaagtgttg
     4201 attcctgaag aagtcggttg ttgtgccttt gcgggtgata aaggttttat gatgccggaa
     4261 ttgaatgctc atgcgttacg aaaattgcgt cctcagattg aaaaatctaa tgtgaaaaca
     4321 ggatatagca acagtaggac ctgtgaaatt ggcttgaata ccaatacagg aatcccttat
     4381 atgtctattg tttatctggt cgataaatgt actaaaagaa aagaattata aatttgttca
     4441 ggaaatttat gaaataattt taattcttga ttaaatttgc cgtataaaat ttattagaaa
     4501 cagttcaatt agtcaacttt atttgaaata tttcaaaaat tttttaaata caaaataatg
     4561 aagaaaatag catttattgt aattttcatt gtactcagtt ttattgggat ttatgcccag
     4621 ccaaagcgtg aaatgcgagc agtctggatt gccacagtag cca
//
LOCUS       DAOSAS010000128         4589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_11877_length_4589_cov_11.445082, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000128 DAOSAS010000000
VERSION     DAOSAS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11877_length_4589_cov_11.445082"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="PLR72_06270"
     CDS             <1..1254
                     /locus_tag="PLR72_06270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK36840.1"
                     /translation="LAEVAWISKSFLLASAVGLLLAGWLADQFGRRKIFLIGNILFGV
                     VSLAIILAENFYIEVALRFIQGLGSSMIMASSPALIASAFPSSRRGFTLGIQTTFTYM
                     GLSLAPLLGGLMTQTLGWKSLFMLNFIFTVLVIIIMLFNIHHEWKNEEISSFDLKGYL
                     LFVVSMISVVFALEWINWKSIVALCIAGIVGYFFVSYELRIDQPFISIPLLKNNKYFT
                     HSVLAALINYATTFAISYVLSLYLQRIRGISPFKSGLILCFQPLMMALFSAYSGHLSD
                     RHNPGKIASYGMMIIAISLFFIGLLLSKTLPLYVLAIVLAVLGFGFALFSSPNTNAVM
                     SSVDTNSYGFASAFLAFNRLFGQFISMALSAALITLFSRQNVSPVPQHSLLIACKIIF
                     IIFAILSCYGVYLSSQRIHSKINSK"
     gene            1384..2457
                     /locus_tag="PLR72_06275"
     CDS             1384..2457
                     /locus_tag="PLR72_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOK36841.1"
                     /translation="MEKNFCIIMAGGIGSRFWPFSRNNRPKQFLDFFGTGRSLLQLTF
                     DRFKKVVPIENILIVSNVIYKDLILEQLPEIQENQLLLEPVRRNTAPCIAYAVNRIKT
                     ITDEANIIVTPSDHLILKEDEFLATIQEGLQFIEKNDCLLTLGIKPSRPETGYGYIQI
                     EPGDGKLRKVKTFTEKPNAELAKIFFESGEFFWNAGIFMWNLRTILAALDFHIPDLAI
                     KFKTIRKYLNTPEEQKYIEEIYPTCPNISIDYGIMEKALNVYVLCSDFGWSDLGTWGS
                     LYEMSPKDKDGNVSLKGKTIFYESNDNIVALTPDKLAVIQDLHGYIVADSDNVLLICK
                     RENEEQIRQFVKDAQILFDGKYN"
     gene            2489..2770
                     /locus_tag="PLR72_06280"
     CDS             2489..2770
                     /locus_tag="PLR72_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36842.1"
                     /translation="MITSFFNIYKPRKYDYRYIYYDPLKEARKERERRMGVTNEDSDK
                     EYRPTLHRGSFREEADKLKKTCTKEVRKTNRNVLIILIILLILAYFLLR"
     gene            2784..>4589
                     /gene="mutL"
                     /locus_tag="PLR72_06285"
     CDS             2784..>4589
                     /gene="mutL"
                     /locus_tag="PLR72_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOK36843.1"
                     /translation="MSDIIHLLPDSVANQIAAGEVIQRPSSALKELIENSIDAEADTI
                     SIQIKHAGRTLIQVVDNGKGMSETDARMAFERHATSKISEINDLYSLHSLGFRGEALA
                     SIAAVSQVELKTRRAEDELGTLIRIAGSRVLEQEPVQCDIGTTVAVKNLFFNVPARRR
                     FLKSDNVEKNHLLYEFYNIALVHPQVSFLFYDEEELIYNLSPSNLKLRIENIFGKNLR
                     KRWEQQLLPIESTTNLVKIYGYVGKPEYAQKSANQYFFVNGRYMRHPYFHKAVLTAYE
                     QMIHENESPNYFIYFEIDPKNIDVNIHPSKTEIKFENEQAIWSILLATIKEALGKFNI
                     IPSIDFNSEGDVKLPPKSSMGNVVPPQPSFNPYFNPFGDTTYQRTPLNWEELYKGFEK
                     NKGMTIEKQSPDETLFDSMKYSSDEKGKIFDNNLLATSYFQLKNHYVLTGIKSGLLII
                     DQHKAHLRILFETFLNQMELQKGFSQQLLFPETLKLTVNDALFFETIIEPLKKTGFII
                     ENKGNNMYEIKGIPASLESVNIIELLHDLIEKSKITDEDPSLHIKETIALTLSKAASL
                     KVGQALTAEEMSGLIDQLFACKDPYYTPDGKKIMIV"
BASE COUNT         1469 a          699 c          809 g         1612 t
ORIGIN      
        1 ttagcggaag tggcatggat ttcaaaatcg tttcttttgg cttcagcagt aggattactt
       61 ttggcaggtt ggttggctga tcagtttgga cgacgaaaaa tatttctcat tgggaacatc
      121 ctttttggag ttgtttcact ggcaattatt ttagctgaaa atttttatat cgaagttgcc
      181 ttgcgattta ttcaaggttt gggcagttct atgattatgg cttcttcgcc tgctcttatt
      241 gcttcagcat tcccttcttc ccgaagagga tttacattgg gtattcaaac tacttttact
      301 tatatggggc tttcacttgc tccattgctg ggtggattaa tgacgcagac attaggctgg
      361 aaaagccttt ttatgttgaa ttttattttc actgtattag ttatcattat aatgcttttt
      421 aatattcatc atgaatggaa aaatgaagaa atttcttcct tcgatctgaa aggatatttg
      481 ctattcgttg tatcgatgat aagtgtagtt tttgcattag aatggataaa ttggaaatcg
      541 atagttgctt tatgtattgc tggaatagtg ggttattttt ttgtttctta cgaacttcga
      601 atagatcaac cttttatttc tatcccttta cttaaaaaca ataagtattt tactcattct
      661 gttttggctg ccttaataaa ctatgcaacg acttttgcta taagttatgt tttaagtcta
      721 tatttacaaa gaattcgagg tatttctcct ttcaaatctg gtttgatttt atgtttccag
      781 cctttgatga tggctttatt ttctgcttat tcaggacatt tatccgatcg gcataatcca
      841 ggcaaaatag cctcttatgg tatgatgatt attgcaattt cactattttt cataggtctt
      901 cttcttagta aaacattgcc attatatgtg ctggcaattg tattagctgt gctaggtttt
      961 ggatttgcct tgtttagttc gcccaatacc aatgccgtaa tgagttctgt cgacacaaat
     1021 tcttacggtt ttgcttctgc ttttctcgct tttaatcgat tgtttggaca atttatatcg
     1081 atggctcttt cagcagcttt gataacatta tttagcaggc aaaacgtttc tcctgttcct
     1141 caacattcat tactaatagc ttgcaaaatt atttttatca tttttgctat tttaagctgt
     1201 tatggtgtct atttatcatc tcaacgaatt cattcaaaga taaattcaaa atagtttttt
     1261 gttatcattt gtttttaaaa acttattttt agattctaag ttttggcaag aaaagaaaaa
     1321 cactgtatct tagtagtgct atttgaatgg cattaataat taacggcttt attcaaaaat
     1381 attatggaaa aaaatttttg tattatcatg gcgggcggta ttggaagccg tttctggcct
     1441 tttagcagaa ataacagacc caaacaattt ttagattttt ttggaaccgg acgatcctta
     1501 ttacaactaa cttttgatag atttaaaaaa gtggtgccaa ttgaaaacat cttgattgtt
     1561 tctaatgtta tttataaaga tttaatttta gagcagcttc ctgaaataca agaaaatcaa
     1621 ttgttgttag aaccagtaag gaggaatacg gcaccgtgta ttgcttatgc tgtaaaccgt
     1681 ataaaaacaa taactgacga agccaatatt attgtaactc cttcagacca tttaatttta
     1741 aaagaagatg aatttttagc aactattcag gaaggacttc agtttattga aaagaacgat
     1801 tgtttactaa ctttgggaat taagcccagt cgtcctgaaa caggttatgg ctatattcaa
     1861 atagaacctg gtgatggtaa attaagaaaa gtgaaaacat tcactgaaaa accgaatgct
     1921 gaattagcaa agattttttt tgaatcagga gaattttttt ggaatgcggg tatttttatg
     1981 tggaatttga gaactatctt agcagcctta gattttcata tccccgatct ggcaattaaa
     2041 tttaaaacaa taagaaaata tctcaatact cctgaagagc aaaagtatat tgaagaaata
     2101 tatccaacgt gtcctaatat ttctattgat tatggcataa tggaaaaagc tttaaatgtt
     2161 tatgtattat gttcagattt tggatggagt gatctgggga catggggttc actttatgaa
     2221 atgtcaccca aagataaaga tggtaatgtg agcttaaaag gtaaaactat cttttatgaa
     2281 agtaatgata acattgtagc tcttactcct gataaattag cagtcattca agatttacat
     2341 gggtatattg ttgctgattc cgataatgta ttattgattt gcaaacgtga aaacgaagaa
     2401 caaattcgtc aatttgtaaa agatgcacaa attttatttg atggtaaata caattaattg
     2461 attcgtttaa taactgattt ttctaactat gattacaagt ttttttaata tttacaaacc
     2521 gcgtaaatat gattatcgct atatctatta tgatcctctg aaagaagccc gtaaagaaag
     2581 agaacgaaga atgggtgtca ctaatgagga tagtgataaa gaataccgac ctactctgca
     2641 tcgtggttcg tttcgtgaag aagctgacaa attaaaaaaa acatgtacaa aagaagttcg
     2701 taaaaccaat cgaaatgtgt taatcatttt gattatttta ttaattcttg cttacttctt
     2761 gttgaggtaa ttttatcttc tgcatgagtg atattattca tttactacct gattcagttg
     2821 ccaatcagat tgctgcaggt gaggttattc aacgtccatc gtccgcattg aaggagttga
     2881 tagaaaattc tattgatgct gaggctgata cgattagtat tcaaatcaaa catgctggac
     2941 gcactttgat ccaggtggtt gataatggaa agggaatgag tgaaaccgat gctcgaatgg
     3001 cttttgaacg gcatgctact tccaaaattt cagaaataaa cgatttatat tctttacatt
     3061 cgttaggctt tcgtggagaa gctttggctt ccattgctgc agtttcacaa gtagaactga
     3121 aaacgcgtcg tgctgaagac gaattaggaa cactaatcag gatcgcaggt tcaagagttt
     3181 tagaacaaga acctgtacaa tgtgatattg gcacgacggt agcagtaaaa aatctttttt
     3241 ttaatgttcc tgcacgtcga cgtttcttaa aaagtgataa tgtagaaaaa aatcatttgc
     3301 tatacgaatt ctataatatt gcactggttc atccccaagt ttcatttttg ttttatgatg
     3361 aagaagaatt aatttataat ctttctcctt cgaatttaaa attaagaatt gaaaatattt
     3421 ttggaaaaaa cttaaggaaa cgctgggaac aacaattgct tccaattgaa tctactacta
     3481 atctggttaa aatttatgga tatgtaggca agccggaata tgctcaaaaa tcggcaaatc
     3541 agtatttttt cgtcaatgga cgttatatgc ggcatcccta ctttcataag gcagttttaa
     3601 ccgcttatga acaaatgatt cacgaaaacg agagtccaaa ttattttatc tattttgaaa
     3661 ttgatccaaa aaatattgat gtcaatattc atccttccaa aacagaaatc aaatttgaga
     3721 atgagcaagc catctggtct atccttttag ctaccattaa agaagcactc ggcaaattta
     3781 atattattcc ttctattgat tttaatagcg aaggtgatgt taaacttcct cctaaatcgt
     3841 caatggggaa tgtagttcca cctcagccat cttttaatcc ttattttaat ccttttgggg
     3901 ataccactta tcagcgaaca cctcttaact gggaagaact ttataaagga ttcgaaaaaa
     3961 acaaggggat gaccattgaa aaacaatctc cagatgaaac tttgtttgat tctatgaaat
     4021 actcctccga cgagaaggga aaaatttttg acaataattt gttagcaact tcttattttc
     4081 aattgaagaa tcactatgtt ttaacgggta tcaaatctgg tttattgata attgaccaac
     4141 ataaagctca cttgaggatt ttgtttgaga cttttttaaa ccagatggaa cttcaaaaag
     4201 gtttttctca gcaattgctt tttcctgaga cattaaaatt aacagtaaat gatgcattgt
     4261 ttttcgaaac tattattgag ccattgaaaa aaacaggatt tattattgag aataaaggaa
     4321 ataatatgta tgagattaaa ggaataccag catctttaga atcggttaat attatagaac
     4381 ttttacatga tttgattgaa aaaagcaaaa taactgatga agatccatct ttacacataa
     4441 aggaaacgat cgctcttacg ctttcaaaag cagcatcgtt gaaagtagga caggctttaa
     4501 cagccgaaga aatgtctgga ttaattgatc agttatttgc ttgcaaagat ccttattata
     4561 cacccgacgg taaaaaaatc atgattgtt
//
LOCUS       DAOSAS010000129         4587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_11886_length_4587_cov_10.902913, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000129 DAOSAS010000000
VERSION     DAOSAS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11886_length_4587_cov_10.902913"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            719..1057
                     /locus_tag="PLR72_06290"
     CDS             719..1057
                     /locus_tag="PLR72_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36844.1"
                     /translation="MIKDLDRDILTIRYNLLNLPDTIQFKNGNQIINQYDAAGRKLST
                     RYYTILIPEYVPLINTLTPGKTIKLAYNMDIIDETGTFYVDNVEYKFNGCDPRTDLQL
                     LPSGSFRQQP"
     gene            993..2027
                     /locus_tag="PLR72_06295"
     CDS             993..2027
                     /locus_tag="PLR72_06295"
                     /inference="COORDINATES: protein motif:HMM:NF026241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M91 family zinc metallopeptidase"
                     /protein_id="HOK36845.1"
                     /translation="MAVILGPTYNYYRRDHLGNNREVWQANTNTTVQRTQYYPSGLPW
                     ATSSGDNPGLQPYKYNGKEFVEMHGYDTYDYGARGMYPPIMRFNTPDPLAEKYYSISP
                     YAYCGNNPVNAVDLLGDSITIIINTTISNPDGTTSLQSTQYYYGQNSNGTYGFIDISG
                     NLYSGTNAFVNSLTTSLNDLQTGGSIGSALVSDLASSTKNVQIVQGNNAADPNGTYIK
                     WNPNSTTGGINQIGTESRPAYIGLGHEMAHIQDSWRGTIDNTTWVTVGETAIPNAEKY
                     ATHIENQLRAENNIPLRTHYGIDASSGKRVGLESTRIINGNLSLFYHQSNGKSPMGIP
                     LLSTSFFYRK"
     gene            2178..2573
                     /locus_tag="PLR72_06300"
     CDS             2178..2573
                     /locus_tag="PLR72_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36846.1"
                     /translation="MRLQKDNVNKLIQWDYLDKLGYSKENDTIYILNMTGVEGDFYFT
                     IWNRSDTLSYTNVSGELIQIERHSFTEYMMKLVSEWNISEIRKEEQINKMTLPERIVY
                     ATKITFNKGEYNIDCLTFNDFFNLKRDSN"
     gene            2940..3410
                     /locus_tag="PLR72_06305"
     CDS             2940..3410
                     /locus_tag="PLR72_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOK36847.1"
                     /translation="MFVKYLGYKKKYNGKEFVEMHGYDTYDYGVRGMYPAIMRFTTPD
                     PLAEKYYSISPYAYCGNNPIKFVDLDGREVVAVSKETQKMILNTLPKEVRQYITFDKN
                     GYINHENIKNIKSNSENFNSLKLMVLSENKTVKVEIDNKFFIKIKMGISKDQKK"
     gene            3407..3766
                     /locus_tag="PLR72_06310"
     CDS             3407..3766
                     /locus_tag="PLR72_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36848.1"
                     /translation="MTYLGIDKDYIGNDNGKGIGTSTGETGFLGKTLFPDNKGLQNSI
                     NDNIIVIVNSNLSEEGKAQVFGHEGYGHAYVYVYVLTNGDRSKSSHNYGTGGKDLNRY
                     LYDMINRAINETIQNMK"
     gene            3763..4317
                     /locus_tag="PLR72_06315"
     CDS             3763..4317
                     /locus_tag="PLR72_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36849.1"
                     /translation="MKGKNILLFVLVILSENLIAQNIETQIYSYSINNSKYSILNLKV
                     ENFTNDSLLLWLENDKRVWNLDNKSKLWNYFFKKKGDFSLINIINEYGSTIENINTTI
                     YYTFYKIILPQETFSIIIQFKQNEQILELNIEDKIKKQLVILSDKDIKKAGINFYNFI
                     DLMKMSFKGDQIILNLKDITSTKN"
     gene            4346..4552
                     /locus_tag="PLR72_06320"
     CDS             4346..4552
                     /locus_tag="PLR72_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36850.1"
                     /translation="MTIKINCSTFTSKNHALQPYKYNGKEFVEMHGYDTYDYGARGMY
                     PAIMRFTTLDPLAEKHYNIFLRIS"
BASE COUNT         1687 a          657 c          817 g         1426 t
ORIGIN      
        1 tttggacgta atcgctctgc taaaggctgc atgaaaatct gttttgaatt actatcacaa
       61 atttagctct aaatttttga ttttttgact tttcagttga aaaattcatg aagtttatag
      121 tttttgagtt ggcaagttat gagattcaag gttgcgagtt tcgagtagaa atatttttga
      181 gaagtgttcg cgtagccttt tgactttgtc gccccaccgt tcggcgtagc ctcaaggcta
      241 catcgagtta aaaataaaat ctccagattt ttaacattgc ttttaataag ctaataaggt
      301 tatattttgc ggagaatttg tttgttacta aggttacagt aaaataaggt ttggtaaata
      361 aatgtatcga aaaatgatgt tcttttaaat tgaccttgtc cggtgagccg aaccaacggt
      421 cagcgtagct aaaaatcagt gaagttggcg ttaaagctct gtcacaaagg atttgtaatc
      481 ctttgctggt ttaatagcgg atttaaaatc cgcaaataat actgtaggat tgcaaatccg
      541 acaggtcaat atttacaaaa ttttagccaa cgaatgtcca tttttcttgt acagacgcca
      601 tatatggtgt ctctactgaa aataataaaa aagtgagtgg ggtttggggc aaagtcccat
      661 tttttgcaaa aagcatccaa cataccttac agaaacaaca aaaaatgcca acggcaatat
      721 gataaaagat ttagaccggg atatattgac gatacgatat aatttactaa atttgcctga
      781 tacgattcag tttaaaaacg gtaaccagat tattaaccaa tacgatgcag ctgggcggaa
      841 attaagtaca aggtattata cgatattaat tcctgaatat gtaccattga ttaataccct
      901 tacacccggc aaaaccataa aattagctta taatatggac attattgacg aaacaggtac
      961 tttttatgtt gataacgtag aatataagtt caatggctgt gatcctcgga ccgacttaca
     1021 attattaccg tcgggatcat ttaggcaaca accgtgaagt atggcaagcc aataccaata
     1081 ccaccgttca gcgaacgcag tattacccga gcggtttgcc gtgggcaacc agttcaggtg
     1141 ataatccggg acttcagcct tataaataca atggcaagga gtttgttgag atgcacggtt
     1201 acgacacgta cgattacgga gcacgaggaa tgtatccccc tataatgcgg ttcaacaccc
     1261 ccgatccact ggcagagaaa tactattcca tcagtccgta tgcgtattgt gggaataatc
     1321 cggtaaatgc ggtagattta cttggcgata gcataactat aattataaat acaacaattt
     1381 ctaatccaga tggaaccaca agtttacaaa gtactcaata ttattatgga caaaattcaa
     1441 atggcacata tggttttatt gatattagtg gtaatttata ttcaggaact aatgcttttg
     1501 taaactcatt aacgacttca ttaaacgact tgcagacagg tggtagtatt ggtagtgctt
     1561 tggtatccga tttggctagt tcaacaaaaa atgtacaaat cgtacaagga aataacgcag
     1621 ctgaccccaa tggaacttac ataaaatgga atccaaactc tacgacagga ggaataaacc
     1681 aaattggcac agaaagcaga cctgcataca ttgggttagg acatgaaatg gctcatattc
     1741 aggatagttg gagaggaacc attgataata ctacttgggt aactgttgga gaaactgcaa
     1801 taccaaatgc agaaaaatat gctacacata tagaaaatca attaagagca gaaaacaata
     1861 tacctttgcg aactcattat ggaatagatg caagttcagg aaaacgtgtt ggattagaga
     1921 gtactcgaat aatcaatgga aatttaagtt tgttctatca tcaatctaat ggaaaaagtc
     1981 caatgggtat ccctttgtta tctacatcat ttttttatag aaaatagtag aatatgaaaa
     2041 aatatttatt cagtataata ttcacctcta tctttttgtg ttgcagtaca tccaaaaaca
     2101 catatcattc caaaatatat gaaaagattt ataattgtca aggtattgac attaataaca
     2161 tcaaacagcc atattatttg agattacaga aagacaatgt aaacaagtta atacaatggg
     2221 attatttaga caaattaggg tattcaaaag aaaacgatac tatttatata ttaaatatga
     2281 caggagtaga aggtgatttt tattttacga tttggaatag aagtgataca ttgtcttata
     2341 cgaatgttag cggagaactt atacaaatag aacgacattc atttacagaa tatatgatga
     2401 aattagtgtc agagtggaat atttctgaaa taagaaaaga agagcaaatc aataagatga
     2461 ctttgccaga aagaatagtt tatgcaacta aaattacttt caataaaggc gaatataata
     2521 ttgattgtct aacctttaat gatttcttta acttaaaaag agactcaaat taagttgtcg
     2581 ttttttaaga gagtgaacac tttttattgc aaattaagaa aaaatagaat aatgcagaat
     2641 tttgtttatt tctgttcttt tcctcgagtt cggcgtaacc tcaaggctac gtcgaattaa
     2701 aaataaaatc tccagatttt caacattgct tttaataagc gaataaggtt tgttttgttt
     2761 ggttttctct tgttatgaag gttgtaaaca aaataaggtt aaaggaattg accttgtccg
     2821 gtgagccgaa aaatcagtga agttggcgtt aaaaatcttt tctgtttgag cgacggagga
     2881 gcgagtttag aagattttag ccaatgaacg tccatttttt cttctaaata aaaaacgtta
     2941 tttttgtaaa gtatcttgga tacaaaaaaa aatacaacgg caaggaattt gttgagatgc
     3001 acggttacga cacgtacgat tacggagtac gaggaatgta tcccgctata atgcggttca
     3061 ccacccctga tccgctggca gagaaatact attccatcag tccgtatgcg tattgtggga
     3121 ataatccgat aaagtttgta gatttggatg gaagggaagt tgttgccgtt tctaaagaga
     3181 ctcaaaaaat gattttaaat actttaccaa aagaagttcg ccaatacatt acgtttgata
     3241 aaaatggcta tataaatcat gaaaatataa aaaatattaa aagtaatagt gagaatttca
     3301 acagtttaaa attaatggtt ttatcagaaa ataagacagt taaagtagaa attgataata
     3361 aattttttat aaagatcaaa atgggaatat ccaaggacca gaaaaaatga cttaccttgg
     3421 tattgacaaa gattatattg gtaatgataa tggtaaaggc attggtactt ctacgggaga
     3481 aacaggtttt ttaggtaaga ccctatttcc tgataataaa ggtttacaaa attcaatcaa
     3541 tgataatatt attgttattg tgaattcaaa tttatctgaa gaaggtaaag cccaagtatt
     3601 tggtcatgaa ggatatggtc acgcatatgt atatgtatat gtattgacca atggagacag
     3661 gtcaaaatct tctcataatt atgggactgg tggtaaagat ttaaatagat atttatatga
     3721 tatgattaac agagctatta atgaaacaat tcaaaacatg aaatgaaggg gaaaaatatt
     3781 ttattatttg ttttggttat attaagtgaa aatttaattg cacaaaacat tgaaactcaa
     3841 atttattctt attctattaa taattcaaag tattccattt tgaatttaaa agtggaaaat
     3901 tttacaaatg atagtctttt attgtggtta gaaaatgata aaagagtatg gaatttagat
     3961 aacaaatcta aattatggaa ttattttttt aaaaagaaag gggatttttc tttgattaat
     4021 ataattaatg aatatggtag cacaattgaa aatatcaata caactattta ttatacattt
     4081 tacaaaataa ttttacctca agagactttt agtatcataa tacaatttaa acaaaatgaa
     4141 caaatattgg aattaaatat cgaagataaa ataaagaagc aacttgtaat cctatctgat
     4201 aaagatatta aaaaagcagg aataaacttc tacaatttta ttgatttaat gaaaatgtca
     4261 tttaagggag atcaaattat tttaaattta aaagatataa cttccaccaa aaactaaaga
     4321 aattagatca aagaaaagca acaaaatgac tataaaaatc aattgttcaa cctttacttc
     4381 caagaatcat gccctccagc catataaata taacggcaag gagtttgttg agatgcacgg
     4441 ttacgacacg tacgattacg gagcacgagg aatgtatccc gctattatgc gattcaccac
     4501 ccttgatccg ctggcagaga agcactataa tattttttta agaatttcat aggctcattc
     4561 ttgagaaaat gaccttaatt ttgataa
//
LOCUS       DAOSAS010000130         4571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_11935_length_4571_cov_8.505757, whole genome shotgun sequence.
ACCESSION   DAOSAS010000130 DAOSAS010000000
VERSION     DAOSAS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11935_length_4571_cov_8.505757"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLR72_06325"
     CDS             complement(<1..187)
                     /locus_tag="PLR72_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36851.1"
                     /translation="MLFSLLGIIIGNNSCSTDEETVIVPKTLEQYKQEFSQFVSMEKE
                     KVQNCKMGYNKGDFKYSS"
     gene            complement(238..1776)
                     /locus_tag="PLR72_06330"
     CDS             complement(238..1776)
                     /locus_tag="PLR72_06330"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOK36852.1"
                     /translation="MKNKIFFAAFLLIIICLTGCEDFLTDKPESVLTQVDFYTTPIRI
                     NQGVIGCYAGLANIQKDEWMYTELRSDNTCQNQTGSSETHRITQTDIDFFRISSSLPD
                     LRDFWYKIFQNISNINAVLPSVADNKHISNENLRAQYEGELLFLRGYHYYILVNLFGD
                     MFKITKVIGPNEAKKIPRSPVSEIYSEIIIPDLKKAAEELPSSYSAADLGRATKWAAK
                     GILAKAYMMLGGSDNLKLAKKELEDILTLSPHDLLTGTGAYANIFSITNEMNKEIIFA
                     VRYKGGSSGIGSPFWGTFAPLGSANQFLKVGTPLGYNNPTYELMNLFNVNPNDTRKDA
                     CFRVWNKSSTVAIPYISKYMDASMTQPLQGENDWIILRFADIKLLYAEILAQDGNFAN
                     AHIPLNDVRVRAGLDGVDPFGSDVEALDAVYAERRLELAFENQRWFDLLRMVKSYNDP
                     DKPMKILKKHTFETDWDILYSKYNPMQPPEERFYIKERLLLPIPQAEIDTNNEMVIPQ
                     NPSY"
     gene            complement(1824..>4571)
                     /locus_tag="PLR72_06335"
     CDS             complement(1824..>4571)
                     /locus_tag="PLR72_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOK36853.1"
                     /translation="AGAAPKIRVRGLGSISASNDPLVVVDGFPIPDGLSMVSMADVQS
                     IEVLKDASSAALYGSRAAGGVILITTKSGEANKPKYNFKMYSGIRNALKLPDMLSTEE
                     YVALLYREAAMRKQDPTVNGIASETMAFNKITEPEKAGYLIMKYLDDQPTDWLDEGLR
                     ERGTMQNYQLSASGGDKNLKYFVSGNYVNENGIMLNSTYDKFTFRAKLDAKLSKIATL
                     GVNLSPTYSRQERPAVDLTDYMRFPSWLPVRHNEATAALTSKVAGDYAQPSDFNGINI
                     SGIGYDNEVWCLPGVTVWSSSNQNPVSIRERTSIFTDDYKFQGNMYLTLNLLPGLQFK
                     TSNGAYLLFREYNKKEQTSANKAGNPNSLTRQLTTHRELLSENTLNYNKKINNHELDV
                     LLGLTMQKTNDNYNRIVGTNFPDEEILSFNLASQILLDSPSIDGTTSFYYTESLVSLL
                     GRINYAYLGKYMLSASFRADGSSKFYKGHKWGTFPAFSVGWRASEEKFMKHFEWLSNL
                     KLRFSYGLTGNNNIPQYAYMNSINTSNYVLGSGNGTLVQGMVSNSTGLGNPDITWEQT
                     SEANYGIDLGFLNSRLNFTVELYNSNTIKLLLQQPAMYITGHQSYWNNIGKVNNKGIE
                     LEVNTTNIANKKFTWRTSANFATNKNTLLNYGDKEYEDNFGERSEVFRAIVGQPAIQY
                     YGYKTDGVYTTFEEVEAAKALKDENGNLFIYTKYAPMVGGVKVLNLNGDNKIDTEDRT
                     VLGDPFPDFTWGITNTFTYRNFDLSFLIQGVQGVDIRNGNVYYNEQLRYNKAYTKNRY
                     VSPMFPGDGKTVYFNTTPGNDLMLTDYCIEDGSYGALRDFTFGYTLPKNLLKSLKLSN
                     LRIYFSAQNLIYIMASDYRGINPEARRTAGNYSSPLVDGYQRGAFPLNRTFTGGLDIN
                     F"
BASE COUNT         1398 a          865 c          731 g         1577 t
ORIGIN      
        1 cgctcgagta tttaaaatct cctttattgt aacccatttt acaattttga actttttctt
       61 tttccattga aacaaactga ctaaactctt gtttgtattg ttccaatgtt tttggaacaa
      121 taacagtttc ttcatcagtt gaacaagaat tattaccaat tataattccc agtaaggaaa
      181 ataacatgaa tatctttttt attcctttca tagccgttgt aaatttatta ttaattttta
      241 ataagaagga ttttgtggaa ttaccatttc attgttggtg tcaatctcag cctgaggaat
      301 aggaagtaac aatctttcct tgatataaaa tctttcttca ggtggttgca ttggattgta
      361 ttttgaatat aatatatccc aatccgtttc aaaagtatgc ttctttaaaa ttttcatggg
      421 tttgtcaggg tcattgtacg atttaaccat gcgaagcaaa tcaaaccaac gctgattttc
      481 aaaagcaagc tccaagcgtc tttctgcata aacagcatcc aatgcttcca catctgaacc
      541 aaatggatca actccatcta atccggctct tactctgaca tcattaagtg gaatgtgcgc
      601 attagcaaaa tttccatcct gtgccagaat ttcggcataa agtaatttta tatcggcaaa
      661 acgcaaaata atccaatcgt tttcaccttg taagggttgt gtcatggatg cgtccatgta
      721 ttttgagata taaggtatgg caacagttga agatttgttc catactctga aacaagcatc
      781 ttttcgagta tcattgggat tgacattgaa caaattcatt aattcgtaag taggattgtt
      841 gtatccaaga ggagttccta cttttaagaa ttgatttgct gaacccaaag gagcaaatgt
      901 tccccaaaat ggagaaccaa ttcctgaact tccgccttta tatcttacgg caaaaataat
      961 ttccttattc atttcgttag taatactgaa aatattggca taagcccctg tccctgttaa
     1021 caaatcatga ggtgataatg tcaagatatc ttccaattct tttttagcca attttaaatt
     1081 gtcgcttcct cccaacatca tatatgcttt ggctaatatg cctttagcag cccatttggt
     1141 agctctaccc aaatcagccg cggaatagct ggaaggtaat tcttcagctg cttttttcag
     1201 atcagggatg ataatctcac tataaatttc agaaacaggt gaacgtggaa ttttttttgc
     1261 ttcatttggt ccaataacct tggttatttt aaacatgtcg ccgaataaat taaccagtat
     1321 gtaataatga tatcctctca aaaacaatag ttccccttca tattgagcac gaaggttttc
     1381 gttactgata tgcttattat cagcgactga tggtaatact gcatttatat tggaaatatt
     1441 ttgaaaaatt ttgtaccaga aatctcgtaa atcgggcaaa cttgaagata tcctaaaaaa
     1501 atcgatatca gtttgtgtaa ttctatgtgt ctcactagaa cccgtctgat tctgacaagt
     1561 attatcactt cttaattccg tatacatcca ttcatctttc tgtatgttag ctaaaccagc
     1621 ataacagcca attactccct gattgattcg tattggggtt gtatagaaat caacctgtgt
     1681 aagtactgat tcaggcttat ctgtaaggaa atcttcacaa cctgttaagc aaatgataat
     1741 cagcaaaaat gctgcaaaaa atattttatt tttcatgttt ttatttttaa taattgatct
     1801 aaactttttc taataagaat tttttaaaaa ttaatatcaa gtcctcctgt aaaagtgcga
     1861 ttcaaaggaa aagcaccacg ctggtaacca tccactaaag gactgctgta attgccggct
     1921 gttcttctgg cttcaggatt aatacctcga tagtcagatg ccataatata aatcaaattt
     1981 tgagcagaaa aataaattct taagttgctt aattttaatg attttaataa attttttggt
     2041 aaagtataac caaaagtaaa atcgcgtagt gctccataag aaccatcttc tatgcagtag
     2101 tcagttaaca tcaaatcgtt tccaggagtg gtgttaaaat agacagtttt tccatcacct
     2161 ggaaacatag gactcacata cctatttttc gtataagctt tattatatcg caattgctca
     2221 ttataatata cattcccatt tctaatatct accccttgta cgccttgaat taagaaactc
     2281 aagtcgaaat ttctatatgt gaaagtatta gttattcccc atgtaaaatc agggaatgga
     2341 tctcctaaaa cagttcgatc ttccgtgtct attttattgt caccattcag atttaaaact
     2401 tttactccac ctaccatagg agcatattta gtataaataa ataaatttcc attttcatct
     2461 tttagtgctt tggctgcttc aacttcctca aaagttgtat aaacaccatc ggttttataa
     2521 ccataatatt gaatagcagg ttgtcctact atagctcgaa aaacttcact tctttctcca
     2581 aaattatctt catattcttt atctccataa tttaaaaggg tatttttatt ggtagcaaag
     2641 tttgctgaag ttctccaagt gaatttttta ttggcaatat tggttgtatt gacttctagt
     2701 tctattcctt tattgttaac tttacctata ttgttccaat atgattggtg accagtaata
     2761 tacatagccg gttgttgaag taacaattta atggtatttg aattataaag ttctaccgta
     2821 aaattcaaac gactattcaa aaaacctaaa tcgattccgt aattagcttc agaagtttgt
     2881 tcccaagtaa tatcaggatt ccccaatcct gtactatttg aaaccattcc ctgaaccaat
     2941 gttccatttc ctgagcctaa cacataatta ctggtgttaa tgctattcat ataagcatat
     3001 tgaggaatat tattattccc agttaaccca taactaaatc tcagttttaa attagataac
     3061 cattcaaaat gcttcataaa tttttcttca gatgctcgcc aacctactga gaaagcaggg
     3121 aaagttcccc atttatgtcc cttataaaat tttgaacttc catctgcacg gaagctagct
     3181 gacaacatat atttgccgag ataggcataa ttgatacgtc ccaaaagcga aactagtgac
     3241 tcggtatagt agaaggaagt tgttccatca attgatggac tgtccagtaa tatttgagaa
     3301 gcgagattaa aagaaagtat ctcttcatca ggaaaattag tgccgactat tctattatag
     3361 ttgtcattcg tcttctgcat ggttaatccc aataaaacat ctaattcatg attatttatt
     3421 tttttattgt agtttaaggt gttttcagaa agcaattctc tatgtgtagt taattgtcgg
     3481 gtcaaagaat tagggtttcc tgctttgtta gcagaagttt gttctttctt gttgtattcc
     3541 ctgaataaaa gatatgctcc attggatgtt ttgaactgca atcctggtaa aagattcaaa
     3601 gttaaataca tattcccctg aaatttgtaa tcatcagtga aaatgctggt tcgttctcta
     3661 atggaaactg gattttgatt acttgaactc caaaccgtaa cacctggtaa gcaccaaact
     3721 tcattgtcgt atccgatccc actaatgtta atgccattaa agtcgctagg ttgggcataa
     3781 tctccagcaa ctttactggt taatgctgct gtggcttcat tgtgtcttac gggcagccag
     3841 gatgggaaac gcatataatc agttaaatct acagccggtc gctcctgtcg gctataagta
     3901 ggtgaaagat ttactcctaa agtggcaatt ttagataact tggcatctaa tttggctcta
     3961 aaggtgaatt tgtcataagt actattcaac atgataccat tttcattgac gtaatttcct
     4021 gaaacaaaat atttcaaatt cttgtctcct cctgaggcac tcaattgata attctgcatt
     4081 gttccccgct ctctcaatcc ctcatcaagc cagtcagtag gttgatcatc caaatatttc
     4141 ataatcagat aacctgcttt ttcgggttca gtaattttgt taaatgccat ggtttcggag
     4201 gctatgccat tgacggtagg atcttgtttt cgcatagctg cttctcgata caaaagtgct
     4261 acatattctt ctgtacttaa catatctgga agtttcaaag cgtttcgaat gccagaatac
     4321 attttgaaat tatattttgg cttattggct tcaccgcttt ttgttgttat caaaataact
     4381 cctcctgctg ctcttgaacc ataaagtgca gcagaagaag cgtctttcaa tacttcaatt
     4441 gactggacat ctgccatact aaccatcgat aagccatcag gaatgggaaa accatctact
     4501 actactaacg ggtcgttgct ggcactgatg gatcccagac cacgaacacg gattttaggt
     4561 gctgctccgg c
//
LOCUS       DAOSAS010000131         4522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_12098_length_4522_cov_12.346541, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000131 DAOSAS010000000
VERSION     DAOSAS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12098_length_4522_cov_12.346541"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..694)
                     /locus_tag="PLR72_06340"
                     /pseudo
     CDS             complement(374..694)
                     /locus_tag="PLR72_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
     gene            1447..2352
                     /locus_tag="PLR72_06345"
     CDS             1447..2352
                     /locus_tag="PLR72_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36854.1"
                     /translation="MKKLLLFIALFGQILVAQTIEVTSVKQLLASSEGGGMYHPVFSP
                     TGDYLLATSENYMGLKMYSFNDQQLKTLTTERGAGYGVQISRDGQTILYRKNELINNR
                     LYISLKSYSLKDQKQQQLIAPTRENIVPKFVGNKPLYVKGNQLVKNQVNNAEISPVIC
                     IENQKMVVYTAKERKVLTPNGENASYIWPTLSPDGKKIAYVVAGKGTFVCDLDGKNVK
                     SLGKLNAPQWLSNNWLVGMNDIDDGEILLSSTLVAATIDGKVYQTLSTPTNIMAMYPA
                     VSPDGKQIVFNTEGGDLYLLNVVLK"
     gene            2368..>4522
                     /locus_tag="PLR72_06350"
     CDS             2368..>4522
                     /locus_tag="PLR72_06350"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOK36855.1"
                     /translation="MKKIISFFIVLMMIFSVKAADFTGIKIYINPGHGGYDGANDRNI
                     QTIPFELGDTLGFWESSSNLAKGLALRDLLQANNATVYMSRTQNREEDDRALSEIAEE
                     ANENNVDAFLSIHSNAIGNNTGTNYLLLLYHGDDNQPTVEASLPMAQTAWPRLISNQL
                     TVWTHYTTSTNIRGDFSFYGNTSGLGVLRPLVVPGFLSEGSFHDYAPETHRLLNVDYK
                     KLEAVNFYRYFCDYFHVDLPTTGIIAGFAKSANEKMNHPLYANYKAFTHDERVPINGA
                     KVKLMNQNGDSLNVYYVDSLYNGIFAFYDLTPGTYKLHLEAKDYEAKDTTVTVTAATT
                     SYAKMFLPNLNVVIPKDTTPDYPDPVQEAGVLPMKHYNFGDEEVVVPEWLNQQPIKKV
                     LYRDEKLYVLTTEPKITIYNAQTLAKIREMDLSGIEGGVNIINDIAFTSDGYLLACNK
                     DTISLPENKGRYFKIYTWDNDSVAPSLLFKTQQQANWSNGIVGETFAVSGPRWKCNIY
                     VPSVTTGSSKQIRIIGFVYEEDNPLGYKYMMDAANYTEALWGENMKFTISPSGKDHFY
                     LDSEKVLPTEYQFDWNADDRSPLIKKGEFSEQNGFNLAVCASGINFFRHAQHTFMVSP
                     TCDADTTKVGAVLFDVTSGIQQSLKVSEKLPANGLGTAKASYMVAAAKVSGYDLDIMV
                     LAQNEGMARFKTIPSPPVANIYASELKAEKTETDYL"
BASE COUNT         1444 a          771 c          863 g         1444 t
ORIGIN      
        1 ttcaaaagga aataaggaaa gtaagttgga atgttaggtg tgctgagttt ttaatcgtaa
       61 gaattccccg atgcttgtgt cggggtagct tttgttttta tttttttaga aattccggtg
      121 gcgaagccat aaaattggcg taatacctcg ctgcttgcgg cgaggatagt caattttaag
      181 gaatttcttt gaaaaaaata gttttaaagt ataattggtt tttgaaagaa gaacttctat
      241 tattttaact ttctactcca tagataaaat aaaagctgca atagcgtttt gtatattaca
      301 atttcttgta atcataccat tctgtaaagt tttacaataa atcattcata atcagcactt
      361 ttctatttca aattcaaatt attcctgttt ctatttcatt gcgaaccaca gccatcatgc
      421 cctccacttg agccaatgca aacattcgtc ccagaaagga atagccggga ttgtaatttt
      481 ttgcgacatc gttgagcata agttttccgt gatcgacacg catgggaaca ttcagattct
      541 ccttctcaaa aattcggatg agttctgtca gatttgccct gccacccaga tgagaagctt
      601 ccacaaaatc gccgttttcc ttgatttccg tgcttcgcaa atgaacgaaa aaagatttct
      661 tggcaaattt tttggccaga tcaaccacgt cattcgtttg aaaaaattgc ttgccgggaa
      721 gaattagaaa tagcaatgga aatagtaaaa gtactcatgc aattctgcat gtgtatttta
      781 tcacatgctg taggtaatat cggcacaggc tgccggcaac aaacctaaaa tggcattgtt
      841 cgaagcattg ctcacgcaat ttgattcccc acaaatacct tgattgcctc acacttgtaa
      901 atccccgaaa gcactgccga caagcaaatt gctcgcttct gcaatgccgg cttattggta
      961 aggcagtcat atgggataca tgcaaaaatt taatcgtaag aattcctcga ggcttgcctc
     1021 ggggttattc atttgttttt atgaaaaaaa taatttcttg cttacagtgt aattattagt
     1081 tgtttatcac atattgaata tttttttatt tttacaaaca tacaaaaaaa ttcaataaaa
     1141 taaccttgtt agaagaaaaa aaatagtatt tttcttgcgt acattaaatt tactggaata
     1201 ttcttaaaag ccgattggta ttggctttta gatcattttt tacaaaattt ggaaaggaaa
     1261 atccgtttta attcccaata atgaagcatt taactatatt gttcaccgct caatttttta
     1321 ctttaaaaaa atattaataa aaattttgaa aataaattag tgcaatcgtt tgcaatcttt
     1381 gtaaaaattc tttatattta ccgccactaa aaatctttaa ttaatttttt aaaatgtaaa
     1441 agtattatga agaaattact cttatttatt gctttgtttg gacaaattct tgttgctcaa
     1501 acaattgaag tgacttccgt gaagcaactt cttgcttctt cagaaggcgg aggtatgtat
     1561 catcctgttt ttagcccaac gggtgattat ttattagcaa ccagcgaaaa ttatatgggg
     1621 ctaaaaatgt attcttttaa cgaccagcaa ttaaaaacat tgactactga aaggggtgca
     1681 ggctatgggg ttcaaataag tagagatggg caaaccattc tttaccgaaa aaatgagttg
     1741 ataaacaatc gcctttacat ttcgttaaaa tcgtattctt taaaagacca gaaacagcag
     1801 caattaattg ctcctacacg cgaaaacatt gttcctaaat ttgtagggaa taagcctctg
     1861 tatgtaaaag gaaatcagtt ggttaaaaat caagtgaata atgccgaaat ttctcctgtc
     1921 atttgtattg aaaatcagaa aatggttgta tatactgcca aggaaagaaa agtattaact
     1981 cctaatggtg aaaatgcaag ttatatttgg cctacacttt cgcccgatgg taaaaaaatt
     2041 gcttatgtcg ttgcagggaa aggaacgttt gtttgtgacc tggatggaaa aaatgttaaa
     2101 tcattaggaa aattaaatgc acctcaatgg ttaagcaata actggttagt agggatgaat
     2161 gatattgacg atggtgaaat tcttctttct tctaccttag tagctgccac tattgatggg
     2221 aaagtttatc aaacactttc cacgccaacc aatatcatgg caatgtaccc tgctgtttca
     2281 cctgatggga aacagattgt atttaatact gagggaggtg atttatattt acttaatgtt
     2341 gttttaaaat aaggaggagg aaacaccatg aaaaaaataa tttcattttt catcgttttg
     2401 atgatgattt tttctgtaaa agcagccgat tttacaggaa ttaaaattta tatcaatcct
     2461 ggtcatggag gatacgatgg cgccaacgat aggaatatcc aaactattcc ttttgaattg
     2521 ggtgatacac tcggattttg ggaatcttcg agcaatctgg caaaaggatt ggcattacgc
     2581 gatttattgc aagctaataa tgctacagta tacatgtcac gtactcaaaa cagggaagaa
     2641 gacgatcggg ctttgtcaga aattgccgaa gaagccaatg aaaacaacgt agatgctttc
     2701 ttatctatac atagtaatgc tattggaaat aatactggca ccaattattt attacttttg
     2761 tatcacggtg atgataatca acccactgtg gaagcgagtt taccgatggc tcaaactgcc
     2821 tggcctcgtt tgatttcaaa tcaacttaca gtgtggacgc attacaccac ctctactaat
     2881 attcggggtg atttttcgtt ttatggaaat acaagtggct taggtgtttt acgtcctttg
     2941 gtggttccag gctttttgtc cgaaggatca tttcatgatt atgctcctga aacgcacaga
     3001 cttcttaatg ttgattataa aaaactggaa gctgtgaact tttatcggta tttctgcgat
     3061 tattttcatg ttgatcttcc aacaacagga attatcgctg gatttgccaa aagtgccaac
     3121 gaaaaaatga atcatccact ttatgctaat tataaagctt ttacacacga cgaacgggtg
     3181 cctatcaatg gagctaaggt aaaattgatg aatcagaatg gagattcact gaatgtatat
     3241 tatgtagatt cgctgtacaa cggaattttt gctttttatg atctgactcc cggaacatat
     3301 aaattgcatt tggaagctaa ggattatgaa gcaaaagata cgactgttac agtaacagcg
     3361 gctactactt cttacgctaa aatgttttta cctaacttaa atgtggtaat tccaaaagat
     3421 accacgcccg attatcctga tccagttcag gaagctggtg tgttaccgat gaagcactat
     3481 aattttggag atgaagaggt agttgttcca gaatggctga accagcaacc tattaaaaaa
     3541 gttttgtatc gtgatgaaaa attatatgta cttactactg aaccaaaaat aacgatttat
     3601 aacgcccaaa cacttgccaa aattcgcgag atggatttat ccggtattga aggtggtgta
     3661 aacattatta acgatatcgc ttttacttcc gatggttatt tgttggcttg caataaagac
     3721 accatttctt tgcctgagaa caaaggtcgt tactttaaga tttatacgtg ggacaacgat
     3781 tcagtagctc cttcattgct ctttaaaacg caacaacagg caaactggtc gaatggtatt
     3841 gtaggagaaa catttgctgt aagtggacca cggtggaaat gtaatattta tgtaccttct
     3901 gtaactaccg gtagcagcaa acaaattcgg atcattggtt ttgtttatga agaagataat
     3961 ccattgggtt ataaatatat gatggatgct gccaattata ccgaagcatt gtggggagaa
     4021 aatatgaaat ttactatttc tccaagtggg aaagatcatt tttatcttga tagtgaaaag
     4081 gtattgccta ctgaatatca gtttgattgg aatgcagacg atcgttctcc tttaataaag
     4141 aagggtgagt tttcagaaca gaatgggttc aaccttgctg tctgtgcttc cggcattaat
     4201 ttcttccgcc atgcacaaca tacctttatg gtttcgccta cttgtgatgc cgatacaacg
     4261 aaagtgggtg ccgtactttt tgatgtgacc agtggcattc agcaatcttt gaaagtttcc
     4321 gaaaaattgc ctgctaatgg actgggaact gcaaaagcca gctatatggt agctgcagct
     4381 aaagtcagcg ggtatgatct ggacattatg gtgttggcgc aaaatgaagg aatggctcgt
     4441 ttcaaaacaa taccgagtcc gcctgtggcc aatatttatg cttcagaatt aaaagccgaa
     4501 aaaacagaaa cagactatct at
//
LOCUS       DAOSAS010000132         4417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_12459_length_4417_cov_10.511233, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000132 DAOSAS010000000
VERSION     DAOSAS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12459_length_4417_cov_10.511233"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(396..1433)
                     /gene="rhaT"
                     /locus_tag="PLR72_06355"
     CDS             complement(396..1433)
                     /gene="rhaT"
                     /locus_tag="PLR72_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOK36856.1"
                     /translation="MNTIIGLIIIAIGSIGQSSFYVPIKKVKNWSWESFWLMQGIFAW
                     LVFPLIGALIAIPEGHSLGEIWLAGDNDIWKCIFYGILWGIGGLTFGLSMRFLGIALG
                     QSLALGTCSAFGTLIPALLKGENLLKGDGLILLISVCIAIAGIAVIGYAGSLKSKILT
                     EEEKKKAVKDFALKKGILIALLAGFMSACFNLGLESGSSIKTALLNIGAKNLMVLNPV
                     IFLVTIGGFLTNAVYCLYQNFKNHTFSDYTHSSGKTWMNNLIFCALAGVLWYSQFFGL
                     GAGQSFFTPGSVMMAFSWSILMSLNVLFSNIWGIILKEWKGVGVKTIFVLIFGLVILI
                     FSIIFPQLIKY"
     gene            complement(1482..2741)
                     /locus_tag="PLR72_06360"
     CDS             complement(1482..2741)
                     /locus_tag="PLR72_06360"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOK36857.1"
                     /translation="MKKDEIIQKSYEIAKERYEAVGVNVDKAIEVLEKLSISIHCWQA
                     DDVTGFENFGNINDAGIQATGNYPGKARNMDELRQDIEKVLTLVGGKHRLNLHAIYGD
                     FQGEKVDRNEIEPSHFTGWMQWAQEKEVKLDFNSTSFGHPKSENLTLANPNKEIRNFW
                     IEHTKCCRLIAEEIGKFQRDPCLMNLWIHDGMKDLTVNRLLYRNILKESLDEIFAKVY
                     TNMKDCIEAKLFGIGLESYTVGSYDFYLAYGTKNQKIVTLDTGHFHLTESIADKISSL
                     LLFVPEIMLHVSRPVRWDSDHVTILNDDVTELAKEIVRADALNRVHVGLDYFDASINR
                     IGAYVIGIRATQKAFLQALLEPLSQLRKYEGNGQYFERLALLEEEKNLPWNAVWDYFC
                     MKNNVPVGEDYIAEIQQYEKEVTSKRN"
     gene            complement(2775..4253)
                     /locus_tag="PLR72_06365"
     CDS             complement(2775..4253)
                     /locus_tag="PLR72_06365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOK36858.1"
                     /translation="MKNSYNFFAVDLGATSGRTILGALQNNTLELKQFTRFQNKILCI
                     HDKYYWNIYSLFEEIKKGLLTASQQGVHIDAMGIDTWGVDFVYLAKDGSLLNLPRSYR
                     DPYTNGIPKEYFSHIPRKRVYDLTGIQIMNFNSLFQLYAARKESSSALEYADSILFIP
                     DAFIYLLTAKKICEYTIASTSQLLNPYTKQIESELLSPMNIEPTIIPQMIMPGTVVGN
                     LVKNIQRECNVPEIPVIAVAGHDTASAVVAVPAEDENFAYLSSGTWSLMGIETKNPII
                     TEESYRLNFTNEGGVESTNRFLKNITGMWLLEKCREEWKLEGKNYTYVEIVQMAENVD
                     SFQFLIDPDDSIFSNPESMTQAITVYCNNSGQKVPITHAELIRCIFDSLALKYKYVLN
                     SLQKLSSFQINRLHVIGGGSQNKLLNQLTANAIGLPVIAGPAEATAMGNIMMQAKGLG
                     LVSSLTEIRSIIRNSVNLEIFYPQNTELWEKAYKKYLHILEK"
BASE COUNT         1507 a          823 c          643 g         1444 t
ORIGIN      
        1 tacaactccg atttcagatc aagaatttat aataggtctt cttgcggata atcatttaaa
       61 aaaataacaa tgtttattga caatatttat atctaagtct gtttgcttac tggcgggtaa
      121 atattgtatg ctatccgttg gaaagtaaag tgatttatta tcaatagtag aaaataagaa
      181 aaaacacttt aaatgaaatc atttacgtgt ggcatgggac taaatccaac aaaaagataa
      241 aaaccattta tcgaattaag atgcaaaaaa ctattttttc atcaaacaaa agtctgtagg
      301 acacagattg aaactcgaaa aaaatgaatc ttcttttttt gtaaattaac aataacagtg
      361 attgtaaggt aagttaaaat tgccttattt atcaattaat attttattaa ttgtggaaaa
      421 ataatggaaa aaatcaatat aactaaacca aaaataagaa caaaaattgt ttttacaccg
      481 actcctttcc attctttcaa tataattccc caaatattgc taaacaacac attcaatgac
      541 ataagtatgc tccatgaaaa agccatcata acacttcctg gcgtaaagaa gctttgacct
      601 gcgcctaaac caaaaaattg tgaataccag agaactccag ccaaagcaca gaatattaaa
      661 ttattcatcc aagttttccc tgatgaatga gtataatcag aaaaggtatg atttttaaaa
      721 ttttgataaa gacaataaac ggcatttgtt aaaaaaccac caatagttac caaaaagata
      781 actgggttga gaaccattaa attttttgct ccaatattca gaagagctgt ttttatagag
      841 cttcccgatt ccaaaccaag attaaaacaa gcactcataa aacctgcaag cagagcaatg
      901 agaattcctt tttttaaggc aaaatctttt acggcttttt tcttttcttc ttctgtcaaa
      961 atttttgatt tgagtgaacc tgcataacct ataactgcaa tgccagcaat ggcaatgcaa
     1021 acactgatta aaagaattaa gccatctcct tttaaaagat tttctccttt taataaggct
     1081 ggtatcaatg tcccaaatgc agaacaagtt cccaaagcca atgattgtcc taaggcaata
     1141 cccaaaaaac gcatactaag gccaaaagtt aaacccccaa ttccccatag aataccgtaa
     1201 aaaatacatt tccaaatatc attatcacct gccaaccata tttctcccag agaatgtccc
     1261 tcaggaatcg ctattaaagc tccaatcaag ggaaaaacaa gccatgcaaa aatgccttgc
     1321 atcagccaga aactttccca agaccaattt ttgacttttt taataggcac ataaaaactt
     1381 gattgtccaa tgctaccaat agcaataatt attaatccga taatggtatt catgatataa
     1441 aaaatataag caaatttgtt aaataattaa gaaatgagaa tctaatttct ttttgaggtt
     1501 acctctttct catattgttg aatttcagca atataatctt ctccgacagg cacattattt
     1561 ttcatgcaga aataatccca aacagcattc caaggcaagt ttttttcttc ttcaagtaat
     1621 gccaaccgtt caaaatattg cccatttcct tcatattttc taagttgtga aagcggttcc
     1681 agcaaggcct gaagaaaagc tttttgagta gcccgtatgc caatgacata cgctccaatg
     1741 cgattgatag aagcatcaaa ataatccaac cctacatgaa cacgattcaa cgcgtcagca
     1801 cgaacaattt ctttagccaa ttcagtaaca tcatcattca atatcgttac atggtcggaa
     1861 tcccaccgta cggggcgact tacatgtaac atgatttccg gaacaaaaag taggagagag
     1921 gatattttat cagcaatact ttcggttagg tgaaaatgac ctgtatccaa tgtaacgatt
     1981 ttttggtttt tagtaccata agccagataa aaatcataag aacctactgt ataactttcc
     2041 aaaccaatgc caaagagttt tgcctctatg cagtctttca tgtttgtata aactttggca
     2101 aaaatttcat ctaacgattc tttcaaaata tttctataaa gcaaacgatt tactgttaaa
     2161 tctttcatcc cgtcgtgaat ccagagattc atcagacaag gatctcgttg aaattttcca
     2221 atttcttcag caattaatcg acaacatttg gtgtgctcaa tccagaagtt gcgaatttct
     2281 ttattgggat ttgctaacgt taaattttca cttttaggat gtccaaaaga agttgaatta
     2341 aagtcaagtt ttacttcttt ctcttgagcc cattgcatcc agccggtgaa atgagaaggc
     2401 tctatttcat tacggtcaac tttttcccct tgaaaatctc cgtaaatagc atgaagattt
     2461 aaacgatgtt tacctcctac caatgtcaac actttttcta tatcttgacg gagttcgtcc
     2521 atattgcgtg ctttacccgg ataattgcca gtagcttgaa tacctgcatc attgatattg
     2581 ccaaaatttt caaaacctgt gacatcatcg gcttgccagc aatgaataga aatagataat
     2641 ttttcgagaa cttctattgc tttgtcaaca tttactccta ctgcttcata acgttctttc
     2701 gctatttcat aagacttttg aataatttca tcctttttca taatatattg attttgtgat
     2761 agttatttca atttttattt ttctaaaata tgaagatatt tcttgtatgc tttttcccac
     2821 aattctgtat tttgaggata aaaaatttcc aaattaacag aatttcgaat gatagaccta
     2881 atttcagtta atgaactaac cagccccaat ccttttgcct gcatcataat gtttcccatc
     2941 gcagtagctt ctgcaggtcc tgcaatcaca ggaagtccaa tagcattagc tgttagctga
     3001 ttaagcaatt tattttgaga gccacctcca ataacgtgaa gtcgatttat ttggaaagaa
     3061 gacaattttt gcaatgaatt tagaacatat ttatatttca atgccaaact gtcaaagata
     3121 caacggatca attcagcatg tgtaatagga actttttgtc cactattgtt acaataaaca
     3181 gttattgcct gtgtcatgct ttcaggattt gaaaaaatac tgtcatcagg atcaattaaa
     3241 aattgaaaac tgtcaacatt ttcagccatc tgaacaattt caacataagt atagttttta
     3301 ccttccaatt tccattcttc acggcatttt tctaaaagcc acattcccgt tatattttta
     3361 agaaaacggt ttgtactttc cactccacct tcattggtaa aattcaatcg ataactttct
     3421 tccgtaataa tgggattttt tgtctcaata cccattaatg accatgttcc cgagcttaaa
     3481 taggcaaaat tttcgtcctc tgcaggtaca gcaaccactg ccgacgctgt atcatgacca
     3541 gcaactgcaa ttaccggaat ttcaggaaca ttgcattccc gctgaatatt ttttaccaaa
     3601 tttcctacaa ctgtacccgg cataatcatc tgtggtatta ttgttggttc aatattcatt
     3661 ggagataata attctgactc aatttgtttg gtataaggat ttaataattg cgaggtagaa
     3721 gcaatagtat attcgcatat ttttttggct gtgagcaaat atatgaatgc atcaggtata
     3781 aataatattg aatcagcgta ttcaagagca gaagaagatt cttttctcgc tgcatacaat
     3841 tgaaacaaac tgttgaaatt cattatttga atgcccgtta gatcgtaaac tctttttctt
     3901 ggaatatgtg aaaaatattc ttttggaatt ccattggtat aaggatcccg ataggaacgc
     3961 ggaagattca gtaaagaacc atcttttgct agatagacaa aatcaactcc ccatgtatct
     4021 atacccattg catcgatatg gactccttgc tgacttgcag ttaaaagtcc ttttttaatt
     4081 tcttcaaaca atgaataaat gttccaataa tatttgtcat gaatacaaag gattttattc
     4141 tggaaacgag taaattgctt taattcaaga gtattatttt gtagcgcacc taaaatggtt
     4201 cgtccactag tggctcctaa atcaactgca aaaaaattat agctattttt cattaaatat
     4261 ttttctaaaa atgcaaaagt tttttataac aatagttcgt taatttttta agcacacaag
     4321 gtgccatata ataaaagttc tttgacattt tggctatttt tgagcaagtt tggatttaca
     4381 gcaaacatgg aaaaaaatct ttcgagcaat aaagcat
//
LOCUS       DAOSAS010000133         4393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_12541_length_4393_cov_13.752882, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000133 DAOSAS010000000
VERSION     DAOSAS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12541_length_4393_cov_13.752882"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            585..3044
                     /locus_tag="PLR72_06370"
     CDS             585..3044
                     /locus_tag="PLR72_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK36859.1"
                     /translation="MKRRILNVVLLVLLSSSLFASVNIKGKVVEESTQLPLEFVNVVL
                     YQVGSSLPLVGVATDKNGNFNMVTDVKGKYLIRISMIGYQPVDKQINIDKSSIDLGII
                     SLKEETKFLSEVEVVAQGFQMKFDIDKKVFSVDQNIASAGGSATEVLQNIPSVDVDNE
                     GNISLRNNSNVEVWINGKPAGLTTENRAQVLQQMPAESIESIEIITNPSAKFDPEGTA
                     GIINLVLKKNRKAGYYGSLSAGLMYPENAKLGKTLGGNFNYSSSKVDAYANIGYRSMN
                     MKGGGWNRRYSYLAMDTTLLSQNNAMNHSFDGLFLRAGADFHLTDKNSIGISGFSMYQ
                     KENSSNFLDYLLTSPIYQYTIRDYSRNTFEKGTHPSYNLTLDYQHDFDKKGTNLIASL
                     TYSNHDRNSEKQFIQREDSLEQATSDITQNSDNQNNNWNFKVDYTNKFTENNKLEAGW
                     QSRIQSRNSLVSGVDNLQQGANIPSYYNNFDYDEQIHAAYVTYGGRFNRLSLQGGVRA
                     EYMWKQITNETYDSIAQLDPQTYFQLFPSIYISYTLPQNGEIQLNYTRRVDRPRGRMI
                     NPFRDYTDSTNISFGNPELKPQFSSSYELNYLKNWYYHSLSFSAYYRSRNNVIQNVRY
                     MHGNNMENTYLNITKTQNSGLEVVVKDNFSNVLNLTSSLNLYFNKLDSATYISPYNTI
                     CHIPSQEDFSWNARVIANVLLAKTFSAQISGNYSSPTIISQGKRKERYSIDLGLRKTF
                     MDRKLNLSLMVRDLLNSRKMRSTTWGEDFYQQTESYFHGRMAGLTISYNFGNMKPASQ
                     KKKSTENNNGEEFPMDNFEGL"
     gene            complement(3067..3861)
                     /locus_tag="PLR72_06375"
     CDS             complement(3067..3861)
                     /locus_tag="PLR72_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005803499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOK36860.1"
                     /translation="MTQNYLKALSKNKIKLISRLQQKKWRDELGCFLAEGTKLVTDLS
                     TSFKGMLLVATPDWLKNHLPIQAEETLIVTSEEFKKISTQKTPQGVLAIFKKKETKWS
                     FLEISSQLCFALEDIQDPGNLGTIIRVADWFGISNIFCSEHSADVYGTKVVQATMGAL
                     ARVQVHIVHLPDFLIESKKYQIPLYGTFLKGENIFTTSLTPEGIIVLGNEGNGISSVL
                     ENIIPTRLTIPSYPSENFTSESLNVSVAAGIVAAEFRKRLMIHKGI"
BASE COUNT         1583 a          617 c          693 g         1500 t
ORIGIN      
        1 gtattaaaat tgtatccaag aaagaaatat catcttttac ttaaataatt tcgttaatag
       61 attgaaaaac aagaggaagt aaatttgcta aaaccaaata caaaataaaa atctttaagg
      121 tttaactaaa aatttaattt ttctcaaaca agatttttag ttgcggattt tatataaagt
      181 ttaaaaatcg gttgcataat attttagtgt tttctcaatg gagaaaaaaa ttataaatat
      241 tttattcgca atagctttat ttcaagaata gtttgtataa tcacaaatta aaatctatat
      301 aacaatgata taaataatcc attttcaatt cattaattag cagttggatt aatattaaaa
      361 agtgtttttc tttttttaac aattaacaac atcgtatcca ttaaacttat ttccattttt
      421 aaagtcaaaa aatataattg ttaattttgt ttttaactta aaactaaatg gggaaaaatt
      481 tggaataaac ctgtagcaat aaaatgaatt atttcaaaat aatttaccta cagatgaatt
      541 tttgttgaca gtatcatttt agcataataa tagaaaataa ctatatgaaa agaaggattt
      601 taaatgttgt tttgttagta ttattaagta gttctttatt tgcctcggtt aatattaaag
      661 gaaaagttgt agaagaatcc actcaactac cccttgaatt tgtaaatgtt gttctttatc
      721 aagtagggtc cagtttacct ttagttggtg tagctaccga taaaaatgga aattttaata
      781 tggttactga tgtaaaagga aaatatctca ttcgtatcag catgattggt tatcaacctg
      841 ttgataaaca aataaatata gataaaagct ccattgattt aggaattatt tctttaaaag
      901 aagaaacaaa atttctttca gaggttgaag tagtagctca aggttttcaa atgaaatttg
      961 atattgataa aaaagtattt agtgtggatc aaaatattgc ctctgcagga ggttcggcta
     1021 ctgaagtttt gcaaaatatt ccttctgttg atgtggataa tgaaggtaat atttcgttac
     1081 gaaacaattc aaatgtagaa gtatggatta atggtaaacc tgctggtttg acgactgaaa
     1141 atcgagcaca agtacttcaa caaatgcctg ctgaaagtat tgaatctatc gaaataatta
     1201 ccaatccttc tgccaaattc gatcctgaag gaacagcagg aattattaat ctggtattga
     1261 aaaaaaatcg taaagctgga tattatggaa gtctttcagc aggacttatg tatcctgaaa
     1321 atgctaaact aggcaaaact ttaggtggaa attttaatta ttctagtagc aaagttgatg
     1381 cttatgccaa cattggttat cgctcaatga acatgaaagg cggaggttgg aatcgtcgtt
     1441 atagttattt agcgatggat accactttgt tatcgcaaaa taatgcaatg aatcattcct
     1501 ttgatggttt gtttttgcga gcgggcgctg attttcattt aactgataaa aattcgattg
     1561 gaatttcggg ttttagtatg tatcaaaaag aaaattcttc caattttttg gattatttat
     1621 tgacaagtcc tatttatcag tataccattc gtgattatag tcgaaatact tttgaaaaag
     1681 gtactcatcc gagttataat ttaacactgg attatcaaca tgattttgat aaaaaaggaa
     1741 ctaatttaat agcaagcctt acttattcta atcatgatag aaattcagaa aagcaattta
     1801 ttcaacggga agatagttta gaacaagcca ccagtgatat tactcaaaat tccgataacc
     1861 agaataataa ttggaatttt aaagtggatt atactaataa atttacagaa aataataaat
     1921 tggaagcagg ctggcaaagc agaattcaaa gtagaaacag tttggtctca ggagtagata
     1981 atttacagca gggagctaac attccttctt attataataa ttttgattat gacgaacaaa
     2041 ttcatgctgc atatgtaact tatggaggcc gttttaatcg tttaagcctt caaggggggg
     2101 tgagagcaga gtacatgtgg aaacaaatta ctaatgaaac ttatgattca atagctcaat
     2161 tggatccaca aacttatttt cagcttttcc caagtatata tatttcttat acgctccccc
     2221 aaaatgggga aatccagttg aattataccc gtagagtgga cagaccgcga ggaagaatga
     2281 ttaatccctt ccgcgattat acagattcca ctaatatttc ctttggcaat cctgagctta
     2341 aaccccagtt ttcttcttct tatgaattga attatttgaa aaattggtat tatcattcat
     2401 tgtcattttc tgcttattat cgttcaagaa acaatgtgat acaaaatgtg cgatacatgc
     2461 atggaaacaa catggaaaac acctatctaa atattacaaa aactcaaaat tcaggtttgg
     2521 aagtagttgt gaaggataat ttttcaaatg ttttgaattt aacttcttct ctaaatctat
     2581 attttaacaa attggattct gctacctata taagtccata taatacaatt tgtcatattc
     2641 cgtcacagga agatttttca tggaatgcaa gagtgatcgc caatgtttta ttagctaaaa
     2701 ctttttctgc tcaaatttct ggaaattatt cttcgccaac cattataagc caagggaaaa
     2761 gaaaagaaag atattcgatt gatctgggat taagaaaaac atttatggac cgtaaattaa
     2821 acctgagctt aatggtgcgt gatttattga attcgcgaaa aatgcgttct actacttggg
     2881 gagaagattt ttatcaacaa acagaatctt attttcatgg acgaatggca ggattaacta
     2941 tttcctacaa ttttggaaac atgaagccag catctcaaaa gaaaaaatcc actgaaaata
     3001 ataatggaga agaatttcca atggataatt ttgaaggatt ataatttttt atccttcaaa
     3061 tttcattcaa attcctttgt gaatcataag tctttttctg aattcggctg caacaattcc
     3121 tgcagccaca ctcacattca atgattcaga agtgaaattt tctgatggat aagatggaat
     3181 agtcagacga gttggaataa tattttccag tacagacgaa ataccattac cttcattgcc
     3241 caacacaatg atcccttccg gggtaagtga agttgtaaaa atattttcac ctttaaggaa
     3301 agtaccatac agaggtattt gatatttctt actttcaatg aggaaatcag gtaaatgaac
     3361 gatatgaacc tgaactcgtg ctaatgctcc catcgtagcc tgaacaactt ttgtcccata
     3421 aacatcagca gaatgttcag agcagaatat atttgaaatg ccaaaccaat cagctacgcg
     3481 tataatagta cctaaattgc ctggatcttg tatatcttcc aaagcaaagc ataattgaga
     3541 agaaatttca aggaacgacc atttagtttc ttttttttta aaaatagcca atactccttg
     3601 aggtgttttt tgtgtagaaa tttttttaaa ttcctcgctt gttactataa gagtttcttc
     3661 tgcttgaata ggtaagtgat tttttaacca atcaggagta gctaccaaca acatgccttt
     3721 gaaagaagta cttaaatctg taactaattt tgtcccttcg gcaaggaaac aacctaattc
     3781 atctctccac tttttttgtt gaagacggga aattagtttt attttgtttt ttgaaagcgc
     3841 tttcaagtaa ttttgagtca tattctgtta taaggggaga tatttttatt agaaaccata
     3901 aaacaaactt tttcaaagga acagagacct aaatggatgc tgaccgatta aagaattttt
     3961 caataatctt aatgacaaat ttacaaaaaa gataatatat ttgttcaaca cttctaattg
     4021 ggtcttaaca atagggggtt aattataatt tgttagctta acgtttaatt ataaacaaga
     4081 gagtattatt attttactaa agtttcgcat ttcagaaaga gttaaaaata aaaattgata
     4141 atcaaagatt taagatactt gtaaattaaa ttagatattt aaaaaagtta ttaattcaaa
     4201 gaaaaacagt atcatacaga aattttctct aaattctatt ttaaaatttc aaaataaata
     4261 aaaggttctt tgattggata agaacctttt attatttttg taaaattaca atttatttta
     4321 attgttttca acaatgggaa atcttatttc tccttcaata ttaacagcag attttagtaa
     4381 tctgcaagca ata
//
LOCUS       DAOSAS010000134         4350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_12692_length_4350_cov_11.602328, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000134 DAOSAS010000000
VERSION     DAOSAS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12692_length_4350_cov_11.602328"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1953
                     /locus_tag="PLR72_06380"
     CDS             <1..1953
                     /locus_tag="PLR72_06380"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36861.1"
                     /translation="LTPQQTRVVEWKVSQFTGFDLVICKVVAQSENFSDGEQKYLPIL
                     PDRILMTESLPITVRGGQTRTFTFESFIRNYSRTETKSLSFEFSDQPVWYAIQALPAL
                     SSPENENAIDYLSAYYVNSLAGFIVSNYPKIAAVFDQWKTSPASKESLLSNLEKNQEL
                     KNLLLEETPWVLEAKDETEQKKRIALLFDLNMQKNNAQQYLEKLLQFQNADGSFSWFK
                     GMPKDRYTTQEILFQLGKLKKLTNKKFDEKLQSSVHSAINYLDLQITHDFFELRKNNK
                     NYLNENCIQPLQLFYLYVRSEFPEISLSDTTAEAINYFSSQAEKYWTKQTLFGKAITA
                     IIAHRNGKDQLANDILNSLKENTLQSETEGMYWAQNKAGYFWNERPIAVQVAIIEAFS
                     EIRANDPAIEEMKIWLLKQKQTQRWDSPLSTISAIYALLSGKEFDLTTDGKVKIQIGN
                     ETLHPETAEAGTQYFKITFPSEKITPSMGKITIEKSSKGIGWGAMYWQHDQDFTTIEN
                     QENSLKIEKKLFVEKLTPQGKNMIPVEQTELKKGDKIITRLVVTADRNFEFVAIKDIR
                     AACLEPLQPISGCRWKEGIYYYQASKDASTQFFFNFLPKGTYVLEYESWINNSGTFSN
                     GIASIQCMYAPEFVNYTGGGKVYVIQ"
     gene            2539..4080
                     /locus_tag="PLR72_06385"
     CDS             2539..4080
                     /locus_tag="PLR72_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOK36862.1"
                     /translation="MFKNQPKGLLAASLANMGERFGFYTMMAILSLFLMAKYGLDETK
                     AGIIYSVFYFSIYILAFVGGLIADKRQNYKETILLGLVLMSLGYFLLTIPTPTPVPSS
                     SFKLYLGLTCFGLLTIAFGNGFFKGNLQALVGQMYDNEQFSKMRDEGFSLFYMFINVG
                     AIFAPFAAIGMRNWWLTSHGFDYHPDLPALCHDQLNGVISSEASLRFQHLATQAGYTG
                     NDLTAFANQYLNLFTTGFHYAFAIAIAAMLISLVIFIVNKKYFPDPQKIIKNSTEEKN
                     FQPLDSKEIKQRLYALFAVFAVVIFFWFSFHQNGLTLTFFAKDYTDLSKISIPLGFTT
                     LRGAEIFQSINPFFVVILTPAIMAIFATLRNKGKEPSTPRKIAFGMGIAATAYILMTI
                     GSIGLPAKNELAELGGLPDSQRVTPFLLLGTYFILTIAELFISPLGISFVSKVAPPQY
                     QGIMQGGWLAATAIGNQLLFIGAVLYKNLPVALTWCVFVAVCLVSMLIMISMLKWLER
                     ITNDI"
BASE COUNT         1412 a          731 c          775 g         1432 t
ORIGIN      
        1 cttacacctc aacaaaccag agtggtagaa tggaaagtat cacaatttac aggtttcgat
       61 ttggtaattt gtaaagtcgt ggctcaaagt gaaaatttca gtgacggcga acaaaaatat
      121 ttacccattt tgcctgacag aatcttgatg acggaaagtt tgcctatcac cgttcgtggg
      181 ggacaaacac gtacttttac ttttgagagt tttatccgaa attattccag aacagaaaca
      241 aaaagtttat cgtttgaatt ttcagaccag cctgtttggt atgctattca agcattgcca
      301 gccttatctt cacccgaaaa tgaaaatgcg attgattatc tttcagctta ttatgtaaat
      361 tcattggcag gatttattgt ttcaaattat ccaaaaattg ctgctgtttt tgatcaatgg
      421 aaaacttccc ctgccagtaa agaatctttg ctctcgaatc tggaaaagaa ccaggagttg
      481 aaaaatttgc tgctggaaga aacgccgtgg gtgctcgaag ccaaagatga aacagaacaa
      541 aagaaaagaa ttgctctatt atttgatttg aatatgcaaa aaaataatgc tcagcaatat
      601 ctggagaaat tattacaatt ccagaatgcg gatggtagct tttcgtggtt taaaggaatg
      661 ccgaaagata gatatacaac gcaggaaatc ctcttccaac tgggtaaatt aaaaaaattg
      721 accaataaga agtttgatga aaaattgcaa tcttcagtcc attcagctat aaattatctg
      781 gatttacaaa taacccatga cttttttgaa ttgagaaaga acaacaaaaa ctatctgaat
      841 gaaaattgta ttcagccgct tcaactcttt tacctgtatg ttcgctctga atttccagaa
      901 atttccttat ctgatactac tgctgaagct ataaattatt tttcttctca agcggaaaaa
      961 tactggacaa aacagacgct atttgggaaa gccataacag ccattattgc ccatagaaac
     1021 ggaaaagatc aactggcaaa cgacatttta aattcgctga aagaaaatac actgcaaagt
     1081 gaaacggaag gaatgtattg ggcgcaaaac aaagccggat atttctggaa cgaacgtcct
     1141 attgctgttc aagtagctat tatagaagct ttttctgaaa taagggcaaa tgatccagcc
     1201 attgaagaaa tgaaaatctg gctgttaaaa caaaaacaaa cgcagcgatg ggattcaccg
     1261 ctttctacca tcagtgccat ttatgctttg ttgtcgggca aagaatttga tttgacaact
     1321 gatggaaaag taaaaattca aataggaaat gaaacgttgc atcctgaaac tgctgaagca
     1381 ggaacccaat atttcaagat cactttcccg tcagaaaaaa taactccatc aatgggtaaa
     1441 atcaccattg aaaaaagtag caaaggaatt ggttggggag ccatgtactg gcaacatgat
     1501 caagatttta caaccattga aaatcaagaa aattcattaa aaatagagaa gaaattgttt
     1561 gtagaaaaat taactccgca aggaaaaaac atgattcctg ttgaacaaac agaacttaaa
     1621 aaaggtgata aaattattac tcggttggtt gtgactgccg atcgaaattt tgaatttgta
     1681 gccataaaag atatacgagc tgcttgtttg gagccacttc aacccatttc gggctgtcgc
     1741 tggaaagaag gaatttatta ttatcaagcc agcaaagatg cctctaccca attctttttc
     1801 aattttttgc caaagggaac gtatgtttta gaatatgaaa gctggataaa caattcagga
     1861 acattttcca atggcattgc ttccattcaa tgtatgtacg ctcctgaatt tgtcaattat
     1921 accggtggtg gaaaagtata tgtaattcaa taatttttag gcaatgaatg gcatttctat
     1981 gttattaatg ttaaattttt ttgataagtt tttattaaaa catgaacaat ttgccgccac
     2041 gagataattt tgaatggtaa aatagatctt ttctctttgc aactcggtga aataatgaat
     2101 ggcaattttg tacaataatt tttcaacctt atttttctat aaccttagta acaagaacaa
     2161 atcctcacaa aacaaacctt attaccttat taaaaattga acaaaatttt gtttagcaaa
     2221 acgtttgatt ttatcagcgg ttaaaatcag gagattttat tttttatgcg acgtagccaa
     2281 gaggctatgc cgaacaatgg gtaactcggt aaaaatgaat aaattgtaga tccgtcgcat
     2341 gtaatggttc tactgaaaat gaataattgg ttgaatgaaa atgatgccaa ttttcaaatc
     2401 ttattatgag gaaaactttc taacaggata taaattccac taaaaacaaa actttaatgc
     2461 catattgtat taagtttaaa ttttgattat tttcgcaagc aagtttaccg tcatattacc
     2521 accataaaca ctaaaattat gttcaaaaat caaccaaaag gtttgttagc agcgtcgtta
     2581 gccaatatgg gtgaacgttt cggtttttat accatgatgg caattttatc tttgttttta
     2641 atggcaaaat atggtctcga cgaaacgaaa gctggcataa tttactctgt attttatttt
     2701 tccatttata ttctggcatt tgttggtggg ttgattgctg acaaacgtca aaattacaaa
     2761 gaaaccattt tattgggttt ggtgcttatg tcattgggtt actttttatt gactatacca
     2821 acgcctactc ctgttccatc aagttctttt aaattatatc ttggattaac ttgctttggc
     2881 ttgcttacca ttgcttttgg taatggattt tttaaaggaa acttgcaggc tcttgtcgga
     2941 caaatgtatg acaacgagca gttttcaaaa atgcgtgacg aaggattttc tttgttttat
     3001 atgtttatta atgttggagc tatttttgca ccttttgcag ccataggaat gagaaattgg
     3061 tggcttacct ctcatggttt tgattatcat cctgatttgc cagctctttg tcatgatcag
     3121 ctcaatggag ttatttcttc tgaagcatcc cttcgttttc agcatttagc aactcaagca
     3181 ggttatacag gcaatgattt aacggctttt gccaatcaat atttgaacct tttcacaaca
     3241 ggctttcact atgcttttgc cattgccatt gcagccatgc tgatttcgct ggtgattttt
     3301 atagtcaata aaaaatattt ccctgatccc cagaaaatta ttaaaaattc tactgaagaa
     3361 aaaaactttc aaccacttga cagtaaagaa attaaacaac gtttgtatgc tttgtttgca
     3421 gtttttgccg tcgtaatatt tttctggttt tcttttcatc aaaatggatt aacgctgaca
     3481 ttttttgcaa aagattatac cgatttaagc aaaatatcta ttcctttggg atttaccact
     3541 ttacgaggtg ctgaaatttt tcaatccatc aatccttttt ttgtagttat tttaacaccg
     3601 gcgattatgg caatttttgc taccttacgg aataaaggaa aagaaccttc cacacctcga
     3661 aaaattgctt tcggaatggg aatagctgct actgcttata ttcttatgac aattggttcg
     3721 ataggtttac ctgcaaaaaa tgaattagct gaattgggtg gtttgcctga ttcacaacgt
     3781 gttacacctt ttttgttatt aggtacttat tttattctaa caatagctga attatttatc
     3841 agtccattag gcatttcatt tgtttccaaa gtagctccac ctcaatatca aggaattatg
     3901 caaggaggtt ggttagcagc caccgctatt ggcaatcaat tattatttat cggagcagtt
     3961 ttgtataaaa atttacccgt tgccttgaca tggtgcgtat ttgtggcagt ttgcctggtt
     4021 tctatgttga ttatgatatc catgttgaaa tggctggaaa gaataaccaa tgatatttaa
     4081 tgactacaga aatatcggtg ctatgcactt tttttgatat agattgcagg caacaattct
     4141 gaacggcgtt gataatttat tttactgaca actttaataa ctggtacaaa ctaccaagat
     4201 aaggttttta tgacagatat ttgtttagca aaacgtttga tttttatcag cggctaaaat
     4261 caggagattt tatttttttg cgacgtagcc tggagggtgc gtcgaacaaa gatatgaggc
     4321 tttgccccaa accccactta cttttttatc
//
LOCUS       DAOSAS010000135         4330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_12774_length_4330_cov_12.579883, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000135 DAOSAS010000000
VERSION     DAOSAS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12774_length_4330_cov_12.579883"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..1449
                     /locus_tag="PLR72_06390"
     CDS             322..1449
                     /locus_tag="PLR72_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK36863.1"
                     /translation="MKKILSFILPLLILVSSCISRNSHRTGETPLCSLHLCYAKGFSV
                     SYFKNFKEVTVFNPWAKEAVYQRYYLVHDTSVTVPVNGVRIKIPVHTLAATSVTHFEF
                     LRLLNEIDAVTGVCSAKIIYNSQINDRLKKGLVVDLGDAFNMNLEKTVQLHADLVMVS
                     GYNQNDPFAQRLVEIGIPVVYNNEWMENSLLGRAEWIKFIGAFFDKEKLADSIFQKIE
                     QQYLEASNKAKGVKYRPTIMSGSNFRGTWYMPGGQNFMAQLFLDAGGNYFYAGDTTKG
                     SLPLNFEKVLKNFAETDMWLNCSYCSLDELLKADAKHALFRPVKLKQVYNFNKRRLSS
                     GANDFWESGIAHPDLLLKDIISILHPELLPDYELFYTKKLE"
     assembly_gap    1466..1565
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1960..2502
                     /gene="hpt"
                     /locus_tag="PLR72_06395"
     CDS             1960..2502
                     /gene="hpt"
                     /locus_tag="PLR72_06395"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOK36864.1"
                     /translation="MNIIQIEDKKFKLYIPEKEILKSIQEIAEKINDDLVDKNPLFIC
                     ILNGAFMFASDLMKAISIPCEITFIKLSSYHGTTTSGKIKEIIGLNEDITKRNIVVIE
                     DIIDTGFTMEFIINSLKSKGANEIQIAALLLKPDALQRDLKIDYAGIKIPNDFIIGYG
                     LDYNGYGRNLRDIYTLISEQ"
     gene            2541..3131
                     /locus_tag="PLR72_06400"
     CDS             2541..3131
                     /locus_tag="PLR72_06400"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOK36865.1"
                     /translation="MLNIILFGAPGSGKGTQSKLIAQKYHLKHISTGELLRTEMKKQS
                     ELGKIAENYILKGNLLPDEIIMDILIKNIDDDLKNYNGILFDGFPRTLPQAEMLEKIL
                     HELNTEITLLIDLKVNEEELIKRLLKRAEIEIRNDDKPDIIKKRLEIYAQNAEPINEF
                     YKKLNKYIAIDGMGTVEEVFSRIEKVINSKLNEAKN"
     gene            3174..4325
                     /gene="obgE"
                     /locus_tag="PLR72_06405"
     CDS             3174..4325
                     /gene="obgE"
                     /locus_tag="PLR72_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOK36866.1"
                     /translation="MATSNFVDYVKIYCRSGKGGAGSTHFRHEKYLPKGGPDGGDGGR
                     GGHIILRGNKNYWTLLHLKYARHIFAEDGENGSKNRSFGKSGQDKIIEVPCGTVVYDG
                     ETGEFICDITQDGEEVILLEGGRGGLGNWHFRTATMQAPRFSQPGEPRKEKTIILQLK
                     VLADVGLVGFPNAGKSTLLSVVSAAKPKIADYPFTTLVPQLGIVSYRDNQSFVMADIP
                     GIIEGAAEGRGLGLRFLRHIERNSILLFMIPADSKNIQEEYYILLSELEKYNPQLLEK
                     ERILAISKSDLLDKNSLKDLKNNLSMDIPSVFISSITGEGINELKNLLWTELNKPSNQ
                     LLEISHQPLSIAHHKIEIEKEEKDDDISFSEENEEENFNQFKGIGWDDQ"
BASE COUNT         1493 a          634 c          722 g         1381 t
ORIGIN      
        1 tttttttaag aatttcatag gctcattctt gagaaaatga ccttaatttt gataatatat
       61 tttatcaata cagtacgttg taattcagat aattataatt cattttttct aacaactgct
      121 attagaaatt ttacttaatg acaaatatga agtaactaaa aaaattgctc gtaagcataa
      181 aaaataaact tttagttttc atgctttgca ttggctgttt tcagtttttc cagctatctt
      241 tgtgaacaac agtgttgatg ttataaattt tgactttttg taagatgggc gtaacattta
      301 tgacgattta agtaaatttt gatgaaaaaa atcttgtctt ttatattacc gttactgatt
      361 ttagtttcct catgtatttc ccgaaattcg catagaactg gcgaaacgcc actatgttct
      421 ttacacctgt gttatgctaa aggtttttca gttagctatt tcaaaaattt taaagaagta
      481 acggttttta atccttgggc aaaagaagcc gtttatcaac gatattattt agttcatgat
      541 acgtcagtta ctgtaccagt gaatggtgtg agaattaaaa ttcctgttca tacattagca
      601 gctacttctg ttactcattt tgaatttctg cgtttactaa acgaaattga tgctgttaca
      661 ggtgtttgct ctgctaaaat tatttacaat tcccaaatca acgacagact taaaaaaggt
      721 cttgtagtag atttgggaga tgcatttaat atgaatcttg aaaaaacagt acaacttcat
      781 gcagatcttg taatggtaag tggttataat cagaacgatc cgtttgcaca gcgtcttgtt
      841 gaaataggca ttccagtagt ttacaacaac gaatggatgg aaaattcctt attgggacgt
      901 gcagaatgga taaaatttat aggtgcgttt tttgataaag aaaaactcgc cgatagtatt
      961 tttcaaaaaa ttgaacagca atatttggaa gcttcaaaca aagcaaaagg agtaaaatat
     1021 cgccctacaa taatgtctgg ttctaatttt cgaggaactt ggtacatgcc aggtggacaa
     1081 aattttatgg cacaactttt tttagatgct ggtggaaatt atttttatgc aggtgataca
     1141 actaaaggaa gtttgccttt gaattttgag aaagtcctaa agaattttgc cgaaactgac
     1201 atgtggttga attgcagtta ctgctcttta gacgaattgt tgaaagcgga cgcaaaacat
     1261 gcactttttc gtcccgttaa attaaagcag gtttataatt tcaacaaacg gaggttgtca
     1321 tcaggagcta acgatttttg ggaaagtggt atagctcatc ctgatttgtt actaaaagat
     1381 ataatttcca ttttgcatcc ggaattatta cctgattatg aattgtttta tacaaaaaaa
     1441 ctagaataaa acattagaat tttaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnaagac ataaagattt atttcttgct cttgtgcagt caaataaaac cctaaataat
     1621 tgaaaccgca aaactcatat ttcgtaccag tatttcgtga tgcacaaact cgcaagtcct
     1681 aaactttttt aacaccaaac agttgatcaa ttatttttat aaatcgatgt ctttctgttt
     1741 atttgagcta aaaaatttat tccgccaaat aatttgattt tcttaagaaa ataacattaa
     1801 ttcatattat cttcttgctt ccttctaatt ataaatgttt ttagtattag tgttttttct
     1861 tatttttgca aattagatga aaattatgca ctttttgtgc attttatgca aaataaaata
     1921 accattatta tatataaaat ggaagattta ccgcactcta tgaatatcat tcaaatcgaa
     1981 gacaaaaaat ttaaacttta tattcctgaa aaagaaatat taaaatcaat tcaggaaatt
     2041 gcagaaaaaa tcaatgatga cctggtagat aaaaatccac tttttatctg tatcttgaat
     2101 ggggcattca tgtttgctag cgatttgatg aaagcaattt ctatcccttg cgaaattact
     2161 tttataaaat tatcttctta tcatggcaca actacttctg ggaaaataaa agaaatcatt
     2221 ggcttaaatg aagatatcac gaaacgaaat atagtagtaa ttgaagatat tattgacaca
     2281 ggctttacaa tggaattcat tataaattct ctcaaatcaa aaggagctaa cgaaattcag
     2341 atagctgctt tacttttaaa acctgatgct ttacaacgag acctaaaaat tgattatgca
     2401 ggtataaaaa ttcccaatga tttcatcata ggctatggat tagattataa tggttatgga
     2461 aggaatttaa gagatattta tactttaatt tctgaacaat agccaaataa cataaaaatt
     2521 ctatataaga caaaattaaa atgctgaata tcattttgtt cggagctcca ggaagtggaa
     2581 aaggaacaca aagtaaactc attgcccaga aataccatct caaacatata tcaacaggag
     2641 aattactacg cactgaaatg aaaaagcaat cagagcttgg taaaatcgct gaaaattata
     2701 ttttaaaagg caatttactt cctgacgaaa taataatgga tattttaatc aaaaatattg
     2761 atgacgattt aaaaaactac aatggaattc tttttgatgg ctttcctcga acacttccgc
     2821 aggcagaaat gcttgaaaaa atactccatg aactaaatac tgaaataacg cttttgattg
     2881 acttgaaagt taatgaagag gaattaataa aacgcctttt aaaacgagca gaaattgaaa
     2941 taagaaacga tgacaaaccg gatataatca aaaaaagatt ggaaatttat gctcaaaatg
     3001 cagagccaat taatgaattt tataagaaat taaacaagta tattgctatt gatggaatgg
     3061 gaactgtgga agaagttttt tctcgtattg aaaaagtaat caactcaaaa ttaaatgagg
     3121 ctaaaaacta aattacctta attttgttat caagttaggt ataaaaaatt cagatggcaa
     3181 cttccaattt tgttgattac gtaaaaatct attgtcgttc tggcaaagga ggcgcaggtt
     3241 ctactcactt ccgtcatgaa aaatatctac caaaaggggg tcctgatggc ggtgatggtg
     3301 gacgtggtgg acatattatt ttaagaggaa ataaaaatta ttggactttg ctacatctaa
     3361 aatatgctcg tcacattttt gctgaagatg gtgaaaatgg cagcaaaaat agaagttttg
     3421 gcaaaagtgg tcaagataaa atcattgaag ttccttgtgg tacagtggtt tacgacggcg
     3481 agacaggaga atttatttgt gatattacac aagatgggga agaagttatt ttgttggaag
     3541 gtggacgcgg tggacttgga aactggcatt tcagaactgc tacaatgcaa gctccgcgtt
     3601 tctctcaacc aggtgagcca cgaaaagaaa aaactatcat tttacaactc aaagtactag
     3661 ctgatgtagg gctggttggt tttcccaatg ctggaaaatc aaccttgtta tcagttgttt
     3721 cggctgctaa accaaaaatt gccgattatc ctttcactac attggttcca caactcggaa
     3781 tcgtttccta tcgtgacaat caatcattcg ttatggctga tattcctggc atcattgaag
     3841 gagcggcaga aggtcgtgga ctgggacttc gttttcttcg acatatcgaa cgcaattcta
     3901 ttcttctgtt tatgattcca gcggatagca aaaatattca ggaagaatat tatattttac
     3961 tcagcgaatt ggaaaaatac aatccacaac ttcttgaaaa agaacgcatt cttgccattt
     4021 caaaaagtga tttgctggat aaaaattcac tgaaggattt gaaaaacaat ctttcgatgg
     4081 atattccttc ggtatttatc tcttctatca caggcgaagg tatcaacgaa ttgaaaaatt
     4141 tactgtggac agaacttaat aaaccatcca atcaactgct ggaaatttct caccaacctc
     4201 tttctattgc tcatcataaa attgagatag aaaaagaaga aaaggacgat gatatttctt
     4261 tttcagaaga gaatgaagaa gaaaatttta atcaatttaa aggaattggc tgggacgatc
     4321 aataattttt
//
LOCUS       DAOSAS010000136         4288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_12951_length_4288_cov_14.013702, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000136 DAOSAS010000000
VERSION     DAOSAS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12951_length_4288_cov_14.013702"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1313
                     /locus_tag="PLR72_06410"
     CDS             75..1313
                     /locus_tag="PLR72_06410"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOK36867.1"
                     /translation="MKIKKIKRNIFYNFKKLLIVNSFFFPAFLNSFISINAQDNRFIV
                     VLDAGHGGEDPGAKGKISNEKDINLAVVKELGKMMEENKELKVIYTRKTDKYLTLQQR
                     ADIANQNHADLFLCIHTNASKNRSAFGAETYTLGLAKTQSNLEVAMRENSVILLEKDY
                     KTKYEGFDPTSVDSYIMFEFMQDKYLDKSIELASLIQTHFNYHGRLDRGVRQAGFWVL
                     HRSACPSVLVELGFISNPEEELFLNSKEGQRKMAEAIYKAFLEYKREHDKRNGISNNF
                     VNTRVETKTDVKQNIPTDISPNQNIAKIEITDSNIVSASPVPLSDNLLDDQAIVFKIQ
                     IFTSRKKLPPNAPDFKGIKEVAFYEEDGLYKYTVGAESDYNKILNLRNEIKTKFPEAF
                     IIAFKGNSKISVKDALKTSK"
     gene            1380..2288
                     /locus_tag="PLR72_06415"
     CDS             1380..2288
                     /locus_tag="PLR72_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOK36868.1"
                     /translation="MKKNFFNKEVKIGLMVIIAIFLLYFGINFLKGIDIFSPTRSYYS
                     VYEKLDGLETSSPVFIKGYKVGQVDKIQYDFSQQKPFVVKISIEKNIRLPEGTKIELF
                     DNGLMGGKALQLILNQSDDEHYFQPGDTIPSQISVGLMGQITNSLLPKIENISSHADS
                     LLTSLRKLSESEKINNSLTSLEETSANLAHSSKQLKNIMNNEIPVIIDNVNTLTTDFK
                     AVSNNIKGIDFASTVGNIDQTINNLNLMSDKINNGEGTLGLLLTDKELYFNLANTTGS
                     ANNLLIDLKNNPKRYVHFSVFGSKTK"
     gene            2705..4081
                     /gene="dnaA"
                     /locus_tag="PLR72_06420"
     CDS             2705..4081
                     /gene="dnaA"
                     /locus_tag="PLR72_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOK36869.1"
                     /translation="MKIHEQLWNRCLKVIKDNINDASFNTWFAPIIPLQYENNCIILQ
                     VPSQFFVEYIEEKYIDLLQMTLYRVIGEGTHLEYRVMIDQSNGKSTQIPSTGEKAKIK
                     LSSSNTTYKKQSLPDIDPQLNLNYNFDTLIEGKSNKLARTAGISIGENPGKTAFNPLF
                     VYGPSGIGKTHLANAIGIKAKQSHADKRVLYVSANTFQIQYTDSVRNNTVNDFLNFYQ
                     TIDVLIIDDIQEFAGKTGTQNTFFHIFNHLHQSGKQLVLTSDRSPLLLAGLEQRLLTR
                     FKWGLSAEIEKPDFELRKAILKNKIYRDGLEIPMDVIDFIALNVTDNVRDLEGVLISL
                     LAHSTLTDVPIEIELAEKIINRIVNVTKKTNTVEKIRDAVCEYFSVSLEDIFTKSRKR
                     EIVQARQIAMYLSKQMTQSSLSYIGNAIGKRDHATVLYACKTVNDLMEIDKSFRNKVK
                     EIEEKLKS"
BASE COUNT         1614 a          622 c          652 g         1400 t
ORIGIN      
        1 aaagataaaa tgatttattc gtttttgaga aaaacaactc tgtataaata aaaatatttt
       61 caggggattt aataatgaaa atcaaaaaaa taaaaaggaa catattttat aattttaaaa
      121 agttactgat tgtcaattct tttttctttc ctgccttttt aaatagtttt atttctatca
      181 atgctcagga taatagattt atagtagttt tggatgctgg acatggaggt gaagatcctg
      241 gagcaaaagg aaaaatttca aatgaaaaag atattaattt agcagtggtg aaagaacttg
      301 gtaaaatgat ggaagagaat aaagaattaa aagtaatcta tactcgtaaa acagataaat
      361 atttaacctt gcagcagaga gctgatattg ccaatcaaaa tcatgcagac ttatttttat
      421 gtattcatac taatgcttca aaaaatcgtt ctgctttcgg agcagaaact tatactttgg
      481 gacttgctaa aacccaatct aacttagaag ttgccatgcg tgaaaactct gtaattcttc
      541 ttgaaaaaga ttacaaaact aaatatgaag gattcgatcc tacttctgtt gattcttata
      601 tcatgtttga atttatgcaa gataaatatc ttgacaaaag tattgaactt gcctccctta
      661 ttcaaactca ttttaattat catggtagat tggatagagg tgttcgtcag gcaggtttct
      721 gggttttgca tcgttcagca tgtcccagtg tccttgttga acttggattc atttctaatc
      781 ctgaagaaga attattccta aattcaaaag aaggacaacg aaaaatggct gaagctattt
      841 ataaagcttt tctcgaatat aagcgtgaac atgataaacg aaatggaatt tcaaataatt
      901 ttgtaaatac aagagtagaa acaaaaacag acgtgaagca aaatatacca accgatattt
      961 ctcccaatca aaacattgca aaaatagaaa taacagattc aaatattgtt tcagcttccc
     1021 ctgttccact ttcagataat ttgcttgacg atcaagctat tgtctttaaa atacaaattt
     1081 ttacaagtag aaaaaaactt cctcccaatg ctccagattt taaaggaatt aaagaggttg
     1141 ccttttacga ggaagatgga ttatataaat atactgtcgg cgcagaatct gactataaca
     1201 aaatactgaa tttacgaaat gaaattaaga ccaagtttcc agaagcattc ataattgctt
     1261 ttaaagggaa cagcaaaatt tctgtcaaag acgctttaaa aacttcaaaa taaaatattt
     1321 tcatttctct atttataagt tgctaattaa gaacggttta aactttaaaa aacttgataa
     1381 tgaagaaaaa tttttttaat aaggaagtta aaattggatt aatggtaata attgccattt
     1441 ttttactgta tttcggaatc aattttttga aaggaattga tattttttca cctacgaggt
     1501 cttattatag tgtttatgaa aagttagatg gtttggaaac ttcctctccc gtttttatca
     1561 aaggctataa ggtaggacag gtggacaaaa tccaatatga tttttctcaa caaaaaccat
     1621 ttgttgttaa aatttcgatt gaaaaaaaca ttcgtttgcc cgaaggaaca aaaattgaat
     1681 tgtttgacaa tggcttaatg ggaggaaaag ctttgcaatt aattttaaat caatcagatg
     1741 acgaacatta ttttcaacct ggcgacacga ttccttctca aataagcgtt ggattaatgg
     1801 gtcaaattac aaacagtctt ttgccaaaaa ttgaaaatat ttcatctcat gcagattcat
     1861 tattgacctc tctcagaaaa ctttctgaaa gtgaaaaaat taataattct ttaacttctc
     1921 tcgaagaaac ttctgctaat cttgcacaca gttccaaaca actaaaaaat ataatgaata
     1981 atgaaattcc agtaataatt gataatgtaa atacacttac aaccgatttt aaagcagtga
     2041 gcaataatat taaaggaatt gattttgctt caacagtcgg taatattgat caaactatca
     2101 ataatttaaa tttaatgtct gacaagataa ataatggcga aggcacgctg ggattgttat
     2161 tgaccgataa agagctttat tttaatctgg caaataccac tggaagcgct aataatttat
     2221 taatcgattt aaaaaacaac ccaaaacgat atgttcattt ttcagttttc ggaagtaaaa
     2281 caaaataaag ttatttagaa tttgtctaaa taagaaagaa atgaaaaaaa taaataatta
     2341 ccttttatta tctttaaaac gcacattaat aatttattca acttgatttt aataaaaaat
     2401 tatagttttt cacactaaat tgttaacatc ttttaaatca ggtacttcta tataaatttt
     2461 gtaacaaact atttgaatct caaataattt tacaaacata ctgattataa gtaaaataaa
     2521 attttgattt ttcttccttt taaaaaatca ttttgatttt tcaaaataaa ttaagatttt
     2581 tgctaaaagg agttgaagtt atctttactt aaaaaattta aatgaattac gcgtaattca
     2641 tttatttttt ctgtgtcaat acaaaattcg taagttatat aacgcgataa tttaattttt
     2701 tgaaatgaag attcatgaac aattgtggaa tagatgctta aaagtcatta aggataacat
     2761 caacgacgca tcatttaata cctggtttgc tcctattatc ccattgcaat atgaaaataa
     2821 ttgtattatt ttacaagtac cgagtcaatt ttttgtagaa tacatagaag aaaaatacat
     2881 cgacttattg caaatgacac tttatcgcgt gataggtgaa ggtacacacc tggaatatcg
     2941 tgtaatgatt gaccaatcaa atggtaaaag cacacaaatt ccaagcacag gtgaaaaagc
     3001 aaaaatcaaa ttgtcttcaa gtaatacaac ttacaaaaaa caatcattac ccgatatcga
     3061 tccccaatta aatttaaact acaattttga tacgttaatt gaaggaaaaa gtaacaaatt
     3121 agctcgaact gctggtatca gtattggaga aaatcctgga aaaacagcct ttaatccatt
     3181 atttgtatat ggaccttcgg gaattgggaa aactcatttg gcaaatgcca tcggcattaa
     3241 agcaaagcaa agtcacgctg ataaacgagt tttgtatgtt tcagcaaaca cttttcaaat
     3301 acaatatact gattctgtac gaaataatac agttaatgat ttcttgaatt tctatcaaac
     3361 tatcgatgta ttaatcattg acgacataca ggaatttgct ggaaaaactg gtacgcaaaa
     3421 tacatttttc cacattttta accatttgca tcaatcaggg aaacaattgg tgctgacttc
     3481 tgaccgttca cctttgttgc tggcaggact tgaacaacgt ttgctaacaa gattcaaatg
     3541 gggactttca gccgaaattg aaaaacctga ttttgaactt cgtaaagcta tcttaaagaa
     3601 taaaatatac agggatggct tggagattcc tatggatgta attgatttta tcgctttgaa
     3661 cgttactgac aatgttcgtg atttggaagg agttttaatt tcattattag cacattctac
     3721 tttaactgat gtacctattg aaatagaatt agcagaaaaa atcatcaatc gaattgtgaa
     3781 tgtaaccaaa aaaacaaata cagtagaaaa aatacgtgat gctgtttgtg aatatttttc
     3841 cgtttctctc gaagatatat ttacaaaaag tcggaaaaga gaaatagttc aggctcgcca
     3901 aattgccatg tatttatcca aacaaatgac acaaagctcg ttatcttaca ttggtaatgc
     3961 aataggtaaa cgcgaccatg ccactgtatt atacgcctgc aaaacagtaa atgatttaat
     4021 ggaaattgat aagagcttta gaaacaaagt caaggaaatt gaagaaaaat tgaaaagctg
     4081 attttaattt attgataata atatttcggc taacccaaat gtaatcaatt cttttgttca
     4141 ttttcgattt caatgaaaaa gagttgaaag tgccgatggt tccataagga ggctgtttgg
     4201 aaatcaaata actatcatat aacttttcat ctttgctgaa ttcaagtcac aaatgcaact
     4261 ttttggttag acaaatttcg taataaaa
//
LOCUS       DAOSAS010000137         4282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_12985_length_4282_cov_12.812160, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000137 DAOSAS010000000
VERSION     DAOSAS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12985_length_4282_cov_12.812160"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PLR72_06425"
     CDS             complement(<1..664)
                     /locus_tag="PLR72_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOK36870.1"
                     /translation="MCGIAMIRLLKPLEYYQKKYGSWEYGLQKMYLLMEKQHNRGQEG
                     AGLACVKLDMPPGEEYISRKRIEGKNAIQEIFQDISQNKEDACEEYSSDTQKAKMLCP
                     FAGELYLGHLRYSTSEREGLKYIHPFLRRHNWKARTLVLAGNFNLTNVDEIFELLTSQ
                     GQHPRDKSDTFLMLESLGDKLDREVQREYDLIKQRYSNDIVITKKIEENLDIASFIRK
                     SER"
     gene            complement(764..2605)
                     /gene="glmS"
                     /locus_tag="PLR72_06430"
     CDS             complement(764..2605)
                     /gene="glmS"
                     /locus_tag="PLR72_06430"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOK36871.1"
                     /translation="MCGIVGYIGDRQAYPILIKGLQRLEYRGYDSAGIALLHDDKLNI
                     YKTKGKVSDLIHYTENKDIEGTIGIAHTRWATHGEPNEINAHPHYSQSKKLALIHNGI
                     IENYSILKEGLTKHGFTFQSETDTEVLVQLIEYMKNKKQVDLSTAVQLALNQVVGAYA
                     IAVIERNHPDVIIAARKSSPLVIGIGENEYFLASDASAIIEYTRKVVYLADEEIVTLE
                     RGKGIKIKTIANVEKTPEVQELEMTLSQLEKGGFPHFMLKEIFEQPKTLSDCMRGRVN
                     VDENNITLSGFIDNKEKFLNAKRIIITACGTSWHAALIGMYAFEEFARIPVEVEYSSE
                     FRYRKPVINKNDVVIAISQSGETADTLAAVQLAKSQGAFIFGICNVVGSSLARNTDSG
                     CYTHVGPEIGVASTKAFTAQVTVLTMLALIIAEEKRTIEKELYLKIIKELGKIPEKIA
                     TILKQNDAIADFSKIFTYAQNFIYLGRGYNYPVALEGALKLKEISYIHAEGLPAAEMK
                     HGPIALIHQEMPVIVIATNSSTYEKIISNIQEIKARKGKIISIITEGDITVKNLSNYT
                     ISIPPAEECLVPLLSVIPLQLLAYHIAIAKGCDVDQPRNLAKSVTVE"
     gene            complement(3031..3633)
                     /locus_tag="PLR72_06435"
     CDS             complement(3031..3633)
                     /locus_tag="PLR72_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOK36872.1"
                     /translation="MSTVLYIKANPKTAYESKTFKISDKFIETYQKYHPYDEIITLDL
                     YKENIDFLTEEDVRIHENNEVEKKDNPMRKYALQFKNADKYVFAEPMWNLGVPAILKA
                     YIDYISIPNITFGYNENGSVGLCQGKKAVNITTSGGDYSEGIADEWEFCDKYLRAIMA
                     LFGVMDYTAIKAYNLDVMGMDVDAIMNKAINEAEELAKRF"
BASE COUNT         1381 a          710 c          638 g         1553 t
ORIGIN      
        1 atcgttctga ttttcggata aaagaagcaa tgtccagatt ttcctcaatt tttttagtaa
       61 taacgatgtc gttgctgtaa cgctgtttga ttaaatcata ttcacgttgt acttctcgat
      121 ccagcttatc gcccaatgat tcaagcatta agaaagtatc tgatttatca cgcggatgtt
      181 gtccttgcga agtaagtaat tcaaaaattt catctacatt tgttaagtta aaattaccag
      241 ccagcacgag tgttcttgct ttccagttat gtcgtcgaag aaaaggatga atatatttta
      301 aaccttctct ttcgcttgta ctgtatcgca aatgtcctaa ataaagttca cctgcaaaag
      361 gacataacat ttttgccttt tgtgtatcag aagaatattc ttcgcatgcg tcttctttat
      421 tttgagaaat atcttgaaaa atttcttgta tagcattttt accttctata cgttttctgg
      481 aaatatattc ttcgcctggt ggcatatcta atttaacgca agcaagtcca gcaccttcct
      541 gacctctgtt gtgttgtttt tccattaata aatacatttt ttggagacca tattcccagc
      601 ttccatattt tttttgataa tattcaagtg gctttaaaag ccttatcatt gcaattccgc
      661 acataataaa caaaaaatta ttaaaaaatt gaattcatta ttctgttttt tcagagaata
      721 aaaaatcaga agtttttcta ttaaaattaa agaatcaaaa atattattcc actgttacag
      781 attttgccag attccgtggt tgatctacat cacatccttt cgcaatggca atatgataag
      841 ccagcaattg taaaggaata accgaaagaa gaggaaccag acattcttca gcaggtggaa
      901 tagaaattgt atagttagat aaatttttta cggtaatatc tccttcagta attatagaaa
      961 taattttccc ttttcttgct ttgatttctt gaatgttgct aattattttt tcataggtag
     1021 aagaattagt ggcaatgaca attactggca tttcttgatg tatcagtgca atgggtccat
     1081 gtttcatttc agctgctggc aaaccttcag catgtatata agagatttct tttaatttta
     1141 aagcaccttc taaagccaca ggataattgt aacccctacc cagataaata aaattttggg
     1201 cataagtaaa aattttggaa aaatctgcaa ttgcatcatt ttgttttaaa atggttgcaa
     1261 ttttctcagg aattttccct aattctttaa taatttttaa ataaagttct ttttcaatgg
     1321 ttcttttttc ttcggcaatt atcaaagcga gcattgtcaa aacagttacc tgtgcagtaa
     1381 atgcttttgt cgaagcaact ccaatttcag gaccaacatg agtataacaa cctgaatctg
     1441 tgttgcgagc gagtgaagaa cctactacgt tgcaaattcc aaaaataaaa gctccttgcg
     1501 attttgctaa ttgaacagca gctagtgtgt cagcagtttc gcctgattgc gaaatggcaa
     1561 ttactacatc atttttattt ataactggtt tacgatatct gaattcagaa gaatattcta
     1621 cttcaacagg aattcttgca aattcttcaa aagcatacat tccaattaaa gctgcatgcc
     1681 aggaagttcc gcaagcagta ataatgattc gtttagcatt taaaaatttt tccttattgt
     1741 caataaagcc tgataaagtg atattgtttt cgtccacatt gactcgccca cgcatacaat
     1801 cactgagtgt tttgggttgt tcaaaaattt ctttgagcat gaagtgagga aatccacctt
     1861 tctcaagttg gcttaacgtc atttcaagtt cttgtacttc aggtgttttt tcaacgtttg
     1921 ctatggtttt aattttaatt cccttgcctc tttcgagtgt cacaatttct tcatcagcaa
     1981 gataaactac tttccgtgta tattcaataa tagcgctagc atcagatgct aaaaaatatt
     2041 cattttcgcc aataccaatc accaatggac tgcttttgcg agcagcaata attacatcag
     2101 gatgatttct ttcgattact gcaatggcat aggcaccaac tacttgattc aacgcaagtt
     2161 ggacagcagt agacaaatct acttgctttt tatttttcat gtattcaata agttggacca
     2221 gaacttctgt gtctgtttca ctttgaaagg taaatccatg ttttgtaagt ccttctttta
     2281 gaatggagta attttcaata attccattat gaatcaaagc gagctttttt gattgcgaat
     2341 aatgtggatg agcatttatt tcattgggtt caccatgtgt cgcccaacgg gtatgagcaa
     2401 ttccaatcgt tccttctatg tctttatttt cagtataatg gatcaaatca ctaactttcc
     2461 ctttcgtttt ataaatattc aatttatcat catgtagcaa agcaatccct gcactgtcgt
     2521 aaccccgata ttccaatcgt tgtaacccct ttattaaaat aggatatgct tgacggtcac
     2581 caatatatcc aacaattcca cacatagtta agtaattaat tttattttta aatagaaaat
     2641 tattacagag ggaggaattt tgaaattcat ttacaatgat ttaaaataga aagagttaaa
     2701 attaaatttc agagaaaaca gtaatttatt ttttcattca ttttaatttg aaaaatcttt
     2761 cccgtcaaat tcctgctttt tctaaaagcc aatgcaaaat tgcgattttt ttttgaggta
     2821 taaaagaatt tttctataaa aaagcaaaaa tgcttatcaa attatttcag atcgtaggaa
     2881 gttaaatata gggtgttatt ggaatggtga agaagttata tttatattac aattaattga
     2941 ttataagcga tttataaatt ttttaaaata aaaaaagcac attttgttgt gctttttagg
     3001 aaggagagat tcatacaaaa aactgattgt tcagaatctt tttgcaagtt cttcggcttc
     3061 gttgatagcc ttattcatga ttgcatcaac atccattccc atgacgtcta aattataagc
     3121 tttaatggca gtataatcca ttacgccaaa tagtgccata atagctctta aatatttgtc
     3181 gcaaaattcc cattcatcag ctattccttc gctataatcg cctccgctgg tagtgatatt
     3241 gactgctttt ttcccttgac ataaacccac agagccattc tcattatatc caaacgtaat
     3301 attgggaata ctaatatagt caatatatgc cttaaggata gccggcaccc ctaagttcca
     3361 catgggttca gcaaaaacgt atttatccgc atttttgaac tgcaaagcat attttctcat
     3421 cggattatct ttcttttcca cttcattgtt ttcgtgaatt ctcacatctt cttcagttaa
     3481 aaaatcaata ttttctttat aaagatctaa cgtaatgatt tcatcatagg gatgatattt
     3541 ttgataagtt tctataaatt tatcagaaat tttgaaagtt tttgattcat aagctgtctt
     3601 tggatttgcc ttaatataaa gaacagtact catataccca cctccttttt aagtgattat
     3661 tatcaaattg ttatttttga gaatttgttt gcaaaagtaa agaaaatatt gggaataagg
     3721 aatgccgaag agtaattttt ctttgaaatt cttgacacta acatagctta atgatcactt
     3781 tatcattcat attgaaagaa ttgctaaaaa acaaataagg gttgttattt tcagtaataa
     3841 attcaaataa taaaaaatga taatttcaat tattgtgaaa aagcagtcaa taaaatgaat
     3901 ctacatttgt cattaggatt gaatttcaaa gggtggttat gagaaaaata ttttataatt
     3961 atttgaatta caatataata tatcaataaa ataatgaata accccgaggc aagcctcgag
     4021 gaattctttt gattaaaaaa attgaggttg tttttcaaaa ttgacgcaag taattcttac
     4081 tcaattctat aaacaaaata taagtacatg agaaaaaaaa ttttaactgt ttaaaatttg
     4141 cttcggcaat taaacatttg tttttgaggc tttacctcca aattctacta actttttgct
     4201 tcaaaagtgt ttatttttaa ttgtttcggc ggtttcgctt tgcttcaatt gttttgatac
     4261 aaatcagaat ttccttataa aa
//
LOCUS       DAOSAS010000138         4232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_13188_length_4232_cov_13.839598, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000138 DAOSAS010000000
VERSION     DAOSAS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13188_length_4232_cov_13.839598"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1492)
                     /locus_tag="PLR72_06440"
     CDS             complement(8..1492)
                     /locus_tag="PLR72_06440"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOK36873.1"
                     /translation="MKTTKKAKAVGLMFLIIIVSVASSLITSYFTNKNKNNNFNQNLQ
                     VPAAYAKFAEVSSAGETDFTVAAELCVHSVVHVKTKSKAQLSFSPDPFFEFFFGTPRL
                     YQEPPVIEGSGSGVIITNDGYIVTNNHVIDRANQIEVTLNDKRTFDAKVVGTDPNTDL
                     ALIKIDADNLPIIPFGNSDSLKVGEWVLAIGNPFNLTSTVTAGIVSAKARNINIINSK
                     MRIESFIQTDAAVNPGNSGGALVNTKGQLVGINTAIASQTGSYAGYAFAIPTSIVKKV
                     VSDLRQYGVVQRAVLGVEIADITAQLAKEKDLKTLEGAYVANVNPGSAAEKAGIKEGD
                     VIINVNGVNVKSVAELQEQIGRYKPGDKISVTVMRKNEKLKFDNVELKNAQGISSVVS
                     ADKINVEKILGATFKEVPASLKKKLDINYGMQVVSLTKGKLLQNGVKVGYIILKINNQ
                     TIRKIEDIQSAVTAALNSEEKVLFLSGIYPDGKITYYAINLEND"
     gene            complement(1780..3057)
                     /gene="eno"
                     /locus_tag="PLR72_06445"
     CDS             complement(1780..3057)
                     /gene="eno"
                     /locus_tag="PLR72_06445"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOK36874.1"
                     /translation="MSRIEKIIAREILDSRGNPTVEVDVTLECGVMGRAAVPSGASTG
                     ENEAIELRDGDKNRYSGKGVLKAVANVNDIIAPALIGMCSLEQRTIDKKMIALDGTKT
                     KSKLGANAILGVSLAVAKAAAAYLDVPLYRYIGGTNTYVLPVPMMNIINGGSHSDAPI
                     AFQEFMIRPVGAPTFREGLRMGAEVFHSLKKVLQERNLSTAVGDEGGFAPTLKGVEDA
                     IESILTAIKKAGYKPGKDVMIGLDCASSEFYENGIYNYSKFEGPNGAKRTSAQQVEYL
                     TELVNKYPIDSIEDGMSENDWEGWKLLTEKLGKKIQLVGDDVFVTNVEFLKKGIEMGC
                     ANSILIKVNQIGTLSETLDAVEMAHRAGYTSVISHRSGETEDSTIADIAVATNSGQIK
                     TGSLSRSDRMAKYNQLLRIEEELGEIAMYGYKK"
     gene            3313..>4232
                     /locus_tag="PLR72_06450"
     CDS             3313..>4232
                     /locus_tag="PLR72_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HOK36875.1"
                     /translation="MHKILIILLMLKQQLQQKLQQKLSPAQIQEIKLLEVTTLELEER
                     IRQELEENPVLEEGPEPELEKEELSDDFEEEKIDEEVNDEIDFEEYLDEDEIPDYKLR
                     INNTSKDDKYEEIPFSAGMTFHDYLMDQLNLIPLTEKEKIIAEFIIGNIDEEGYLRRK
                     PEALIDDLAFQLNIETNEEKIRKIIKIIQQLDPPGVGAYDLQECLLLQLERKPFTPEI
                     DIAKRIVKEYFKEFTRKHFEKLIKGLEIDEEKLKKAMNEIIKLNPKPGSAWSGSVLEK
                     SMTTVVPDFILENKDGELYVHLNNSNIPELR"
BASE COUNT         1409 a          753 c          647 g         1423 t
ORIGIN      
        1 ggtcttttta atcgttttcc aaattaatag cataataggt aattttccca tcgggataaa
       61 tgccgctgag gaaaagaact ttttcttcac tattcaaggc tgctgttaca gcactctgta
      121 tgtcttctat ttttcgaata gtttgattgt ttattttcaa aataatatat cctactttta
      181 caccattttg aagtaattta cctttagtaa gtgaaactac ttgcatacca taattaatat
      241 ccaatttttt ctttaaagaa gctggaactt ccttgaaggt agcacctaaa attttttcaa
      301 catttatctt atcagcagaa acaacacttg aaattccttg tgcatttttt aattctacat
      361 tgtcaaattt cagtttttca tttttacgca tcactgtaac agaaatttta tcgcctggtt
      421 tatagcgtcc aatttgttcc tgaagttctg ctactgattt tacatttact ccattgacgt
      481 tgataattac atcaccttcc ttaattcctg ctttttctgc agcacttcct ggatttacat
      541 tagctacata agctccttcc aacgttttta aatctttttc ttttgctaat tgtgcagtaa
      601 tatcagcaat ttctacaccc aatactgctc tttgaactac tccatattga cgaagatctg
      661 aaaccacttt tttcacaatg ctggttggaa tagcaaaagc ataaccagca taagaacctg
      721 tttgtgatgc aatggcggta ttaattccta ctaactgtcc tttagtattt acaagggctc
      781 cgccactgtt tcctggattg acagctgcgt cggtctgaat aaatgattca attctcattt
      841 tggaattgat aatattgata ttacgggctt ttgcactgac aattcctgct gtaacagtag
      901 aagtaagatt aaaaggattg ccaattgcca gtacccattc acctactttc agtgaatcag
      961 aatttccaaa gggaataatc ggtaaattgt cagcatctat ttttatcaag gcaagatcag
     1021 tattcggatc tgtccctact acttttgcat caaaagttct tttgtcattt aacgttactt
     1081 caatttgatt agcacggtca atgacatgat tgtttgtaac tatataacca tcatttgtaa
     1141 taattactcc tgaacctgag ccttcaataa cagggggctc ttgatagagt cttggtgttc
     1201 caaaaaagaa ttcaaaaaaa ggatcaggac tgaaggataa ttgagctttt gattttgttt
     1261 taacgtgaac cactgaatgt acacaaagtt ctgcagctac tgtgaaatcc gtttctccag
     1321 cactggatac ttcagcaaat tttgcatacg cagcaggaac ttgaagattc tgattaaaat
     1381 tattgttctt gtttttattt gtaaaataac tcgtaataag actggaagct acactcacaa
     1441 taataatcaa aaacatcaaa ccaactgctt tcgctttttt tgttgtcttc atttgttgtt
     1501 tttttaaaag ttatttttaa tataaatttt tgtgatttac aaacaaaaat aatacctaaa
     1561 attgataatg aacatttgtg tttaaataaa tttatgtttt ataacaacag ttttgatata
     1621 gatttgttta aaattaactg ataaattcgt tctttgattg atattaacat aatagtggat
     1681 ttttcaaaaa tttacaaaag ctaagaagag ctttataaaa aagaaccact tattacagtg
     1741 gctcttctca aaaaattgac aggagaatga caatttttct tattttttat aaccatacat
     1801 tgcaatttcc ccaagctctt cttcgatacg caacaactga ttatatttag ccatacggtc
     1861 agaacgactt aatgaaccag ttttgatttg tcctgaattg gttgctactg caatatcagc
     1921 tattgtagaa tcttctgttt cgcctgaacg atgtgaaatg acggatgtat atccagcacg
     1981 atgtgccatt tcaacagcat caagcgtctc acttaaagtg ccaatttgat ttaccttaat
     2041 taaaatggaa tttgcacaac ccatttcaat gccttttttc aagaattcaa cattggttac
     2101 aaatacatca tcaccaacta attgaatttt tttgcctaac ttttctgtta aaagtttcca
     2161 accttcccag tcattttcgc tcatcccatc ttctatagaa tcaataggat atttattgac
     2221 caattctgtc agatattcaa cttgttgagc tgaagtgcgt tttgcaccat tgggcccttc
     2281 aaatttagaa taattataaa taccattttc ataaaattca gaagaagcac aatcaagccc
     2341 aatcatcaca tcttttcctg gcttataacc agctttttta atggcagtca atattgattc
     2401 aatagcatct tcaactccct ttaaagtagg agcaaaacca ccttcgtcac ctacagcagt
     2461 actcaaattt ctttcttgca agactttttt caatgaatga aaaacttcag cgcccattcg
     2521 aagcccttcg cggaatgtag gagcacctac cggacgaatc atgaattcct gaaaagctat
     2581 gggggcatca gaatgagaac caccattgat aatgttcatc atggggacag gcaaaacata
     2641 agtatttgtt ccaccaatat aacgatacaa aggaacatca agataagcgg cagcagcttt
     2701 tgcaacggct aaagaaactc ccaaaatagc attggcgcct aactttgatt tcgtttttgt
     2761 accatccaaa gcaatcattt ttttatcaat tgttcgttgc tctaatgagc acatgccgat
     2821 taatgctgga gcgataatat cattcacatt tgcaacggct tttaatacac ctttgccaga
     2881 atagcgattt ttgtcaccat cgcgtaattc aatagcttca ttttcgcctg ttgaagcccc
     2941 tgaaggaaca gcagcacgtc ccattacacc acattccaat gttacatcta cttcaactgt
     3001 cggattgcct cttgaatcca atatttcacg ggcaataatt ttttcaattc tactcatatt
     3061 ttttaaattt taattaattt agaaagattt tgataatcat tttttttctg atgcaaagta
     3121 acaaaatgtt ctcataatat tatttgaata aatcattaaa atttcaaaaa gtatgttttt
     3181 tcatggatta aaaaaattaa tttcaaaagc tatttcaaat tcccaaaatg atttagaaat
     3241 taaaaaaata tcagatattt taactttcac aaatatttat gtaataaact tatttgcaaa
     3301 aaacttttat ctttgcataa aatacttatt attttactga tgttaaaaca acaattacaa
     3361 caaaaattac aacagaaact ttcgcctgcc caaattcaag aaataaagct actcgaagtg
     3421 actactttag agttagaaga acgaatacgc caggaattag aagaaaatcc agttctggag
     3481 gaaggtccgg aacccgagct cgaaaaagaa gaactttctg acgattttga agaagaaaaa
     3541 attgatgagg aagtcaatga tgagattgat tttgaagaat atcttgatga agatgaaatt
     3601 cctgattata agcttagaat taataatact tcaaaagatg acaaatatga ggaaattcct
     3661 ttttctgcgg gtatgacttt tcatgattat ttgatggatc aattgaacct aattcctctt
     3721 acagagaaag aaaaaattat tgccgaattt attataggaa atatagatga agaaggatat
     3781 ttacgtagaa agcctgaagc acttattgat gatttggctt ttcagttaaa tattgaaaca
     3841 aatgaagaaa aaatcaggaa aattatcaaa atcattcaac agcttgatcc tccaggtgtc
     3901 ggagcttacg atttacaaga atgtttgctg cttcagttag aacgtaaacc ttttacgcct
     3961 gaaattgata ttgccaaacg tattgtaaaa gagtatttta aagaattcac aagaaaacat
     4021 tttgaaaaat taatcaaagg gttggaaatt gatgaggaaa aactaaaaaa ggcaatgaat
     4081 gaaataataa aacttaatcc gaagcctgga agtgcctgga gcggcagtgt gctggagaaa
     4141 tccatgacca cagtagtgcc tgattttatt ctggaaaaca aagatgggga actttatgtg
     4201 catttaaaca atagcaatat tcctgagtta cg
//
LOCUS       DAOSAS010000139         4220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_13231_length_4220_cov_10.613685, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000139 DAOSAS010000000
VERSION     DAOSAS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13231_length_4220_cov_10.613685"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLR72_06455"
     CDS             <1..444
                     /locus_tag="PLR72_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOK36876.1"
                     /translation="TSGRAARNINGKVIMYADTITESMKRTIEETNRRREKQLKYNEM
                     HHITPTSIVKGERNSLLKTEPQAYIEPEPQNAVAADPVVQYMSATDLEKTITKTKKAM
                     QEAAKNLEFLEAAQLRDELFKLEELLRTKSSHSSAAKKEFSKRKK"
     gene            748..1185
                     /locus_tag="PLR72_06460"
     CDS             748..1185
                     /locus_tag="PLR72_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOK36877.1"
                     /translation="MAITRFTEQVPQFYDPFFNDDLFDWMTSNFFGTDITLPAVNIKQ
                     GPDAFTVEVAAPGYKKDDFKIKMEDDVLTIASEKEPKEELKEGERYTKREFNYGAFTR
                     SFVLPDIVEGDHISASYEDGLLRITLPIKEEAKQKPARMIEIQ"
     gene            1256..1552
                     /gene="trxA"
                     /locus_tag="PLR72_06465"
     CDS             1256..1552
                     /gene="trxA"
                     /locus_tag="PLR72_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOK36878.1"
                     /translation="METFQEIINGEKPVLVDFFATWCGPCKAMSPIIESVGKEVQGKA
                     RVLKVDVDKNPAVAAKYGIQAVPTIILFKQGKILWRNAGVTDKQTLLQQIQAAI"
     gene            1643..1716
                     /locus_tag="PLR72_06470"
     tRNA            1643..1716
                     /locus_tag="PLR72_06470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1677..1679,aa:Arg,seq:ccg)
     gene            1888..3300
                     /gene="rlmD"
                     /locus_tag="PLR72_06475"
     CDS             1888..3300
                     /gene="rlmD"
                     /locus_tag="PLR72_06475"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOK36879.1"
                     /translation="MMKKKKQLPILEKVTITDIGAEGKAIAKVNNCVVFVPYVVPGDV
                     VDLQVTQKKKSYMEARAVKFHQYSEKRCQPICQHFGVCGGCSWQMLPYSEQLFYKQKQ
                     VEDHLLHIGKIQFPPLLPILSSDKTEFYRNKLEFAFSNKRWKTSEEMQQQEKTSKNEN
                     AVGFHIPGKFDKVLDIEKCWLQDDISNQIRNEVRRYAMEKGFSFFDLRRQEGFLRTMI
                     IRTSSTGEVMVIISFFQENKKWREDLLQYLADTFPQITSLLYVINPKANDTIADQKIN
                     VFKGNEYIFEVMEDLKFKIGPKSFFQTNTAQACKLYQVVKNFAQLTGKELVYDLYSGT
                     GTIANFMARHCKKVIGIEYVPEAVIDAIANSDLNQIKNTSFFAGDMKKIMTPEFIQQQ
                     GQPDVIITDPPRVGMHADVIKAILQTHTQRIVYVSCNSATQARDLNLMDAQYHIVAIQ
                     PVDMFPHTHHVENVVLLEHR"
     gene            3359..>4220
                     /locus_tag="PLR72_06480"
     CDS             3359..>4220
                     /locus_tag="PLR72_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOK36880.1"
                     /translation="MISFFISIGLLIAGYFIYGKIAERIFGIDPNRPTPALANPDGVD
                     YIPLPWWRVFLIQFLNIAGLGPIFGAIMGIMFGESAFLWIVFGTIFAGGVHDYLSGMM
                     SIRNNGASLPELVGNELGNGIKQFMRAFSLALMILVGAVFVSGPAQLLANFTPHAINV
                     SGWIVIVFTYYILATLLPIDKIIAKIYPFFGFVLLFMAVGIFLALILNKAPIPEFTEG
                     FANLHPNKLPIFPIMFVSIACGAISGFHATQSPLMARCIQNEKLGRRIFYGSMVAEGI
                     VALIWAAAASS"
BASE COUNT         1423 a          652 c          770 g         1375 t
ORIGIN      
        1 acctctggaa gagcagctcg caatataaat gggaaagtta ttatgtatgc tgatactatt
       61 acagaaagca tgaaaagaac cattgaagaa accaatcgac gtagagagaa acaattgaaa
      121 tataatgaaa tgcatcatat caccccgact tctattgtaa aaggtgaaag aaattcctta
      181 ttaaaaacag aacctcaggc ttacatcgag ccagagcctc aaaatgctgt tgctgctgat
      241 cctgtggttc agtacatgtc tgcaactgat ttggaaaaaa ctataacaaa aactaaaaaa
      301 gcaatgcaag aagcggcaaa aaatctagaa tttttagaag ctgctcaact cagagatgaa
      361 ttatttaagc tggaagaatt attgagaact aaatcttccc attcttcagc agcaaaaaag
      421 gaattttcaa aaagaaaaaa ataatttttg aagatatata ccttattttt aatgaattgt
      481 tatcaaattg aatctttaat ttaataatat taacaaatta acctaatttt gcaatttacg
      541 taattgaaga aaaaaattgt tatttagtta ttgtcttaaa aagtacagta aatccatttt
      601 caaaaacgaa attttattaa cctgacgaat ttgcttttct gttctaaaaa aaaatatgtc
      661 aaaatgtcta atttatgttc tggtattctg tttgttgaga aataaatgaa acatatataa
      721 accattaaaa ggaaggaggg ttaaattatg gcaatcacaa gatttacgga acaagtgcct
      781 caattttatg atccattttt caatgacgat ctttttgatt ggatgacttc taactttttt
      841 ggcactgata taacattacc ggctgtaaac atcaaacaag gtcctgatgc ttttaccgtt
      901 gaagtagcag ccccaggata taaaaaagat gatttcaaaa tcaaaatgga agatgatgtt
      961 ttaaccattg cttctgaaaa agagccaaaa gaagaattga aggaaggaga aaggtacacg
     1021 aaacgtgaat tcaattacgg agcatttact cgttcatttg tattgcctga cattgtagaa
     1081 ggcgatcata tctcagcaag ctatgaggac ggattgttac gcattacact tccaattaag
     1141 gaagaagcaa aacaaaaacc agctcggatg attgaaattc aataaggaat gagaaagtta
     1201 aaaaagtaca aaaagggaag ccgaaaagtt tccctttttt aacaataatg attttatgga
     1261 aacatttcaa gaaataatta acggagaaaa accagttttg gtagattttt ttgcaacatg
     1321 gtgtggaccg tgtaaagcaa tgagtcccat tattgaaagt gtgggaaaag aagtacaggg
     1381 gaaagctcgt gttttaaaag tagatgtgga taaaaatcca gcggttgcag caaaatacgg
     1441 aattcaggca gttccaacaa ttatcttgtt caagcaggga aaaattcttt ggagaaatgc
     1501 gggtgttacg gataaacaaa ctttacttca acaaattcag gctgccattt aaattcaaat
     1561 ttgttggtaa gtagaaaatt ctcccaaaaa aattctactt agtataaatc gagaaaaaat
     1621 aagtatcttt gtacttacaa aaggctccgt agttcagttg tatagaacgt cagattccgg
     1681 ttctgaaggt cttgggttag aatcccaacg gggtcacata cataaccaac ttcaacattt
     1741 ttgttcgggt tggttttttt gttataataa aaaatttttt atataattaa ttatcagttg
     1801 attaactttc tattttattt ctcctatccg attttcaacc aaattcatta attttgcaga
     1861 aaaatttgga ttttgaattc agcataactg atgaagaaaa aaaagcagct tcccattctt
     1921 gaaaaggtaa cgattactga tattggtgcc gaaggtaaag ctattgcaaa agtgaacaat
     1981 tgcgtagttt ttgtacctta tgtagtgccg ggtgatgtgg ttgatttgca ggttacacag
     2041 aagaaaaaaa gttacatgga agcccgagct gtgaaatttc atcaatattc tgaaaaacgt
     2101 tgtcaaccta tttgccaaca ttttggcgtt tgtggtggtt gtagttggca aatgcttcct
     2161 tattcggaac aattatttta taaacagaaa caggtggaag atcatctttt acacattggc
     2221 aaaattcaat ttccgcctct tttacctatt ttatcatctg ataaaacgga attttatcga
     2281 aacaaattgg aatttgcttt ctcgaataag cgatggaaaa cttcagaaga aatgcagcaa
     2341 caagaaaaaa cttcaaaaaa tgaaaatgct gtaggatttc acattccagg aaaatttgac
     2401 aaagtactgg acattgaaaa atgttggctg caagatgata tttccaacca aatcagaaat
     2461 gaagtcagaa gatatgcaat ggaaaaaggt tttagttttt ttgatcttcg acgtcaggaa
     2521 ggttttttaa gaacaatgat tattcgaact tcttcgacag gagaagtaat ggtcatcata
     2581 agtttttttc aagagaataa aaaatggcgt gaagatttac tgcaatatct tgcggatact
     2641 tttccccaaa ttacatcact tttgtatgtg attaatccaa aagctaatga caccatcgcc
     2701 gatcagaaaa taaatgtttt caaaggcaat gaatatatct ttgaggtaat ggaagatttg
     2761 aaatttaaaa ttggtccaaa atcttttttc cagactaaca cagctcaagc ctgtaagctt
     2821 tatcaagtag taaaaaattt tgctcaatta acaggtaaag agctggtata tgatttatat
     2881 tctggaactg gaaccattgc caattttatg gctcggcatt gtaaaaaagt cattggaatt
     2941 gaatacgttc ccgaagcagt aatagatgcc atagccaatt ctgatttgaa ccaaattaag
     3001 aatacttctt tttttgccgg cgatatgaaa aaaattatga cacctgaatt tattcaacaa
     3061 caaggtcagc cagatgtaat tattactgat ccaccacgtg taggtatgca tgcggatgtt
     3121 atcaaagcca ttcttcaaac gcatactcag cgaatagtat atgtgagttg taattctgct
     3181 acgcaagctc gtgatttgaa tttaatggat gcacaatatc atattgttgc tattcaacct
     3241 gtggatatgt ttcctcatac ccatcacgtt gaaaatgtag tgttacttga acatcggtaa
     3301 acaaaatatt ttaaaaatat atttgaataa aaactcatag cttaaatttt aaattatcat
     3361 gattagtttt tttatttcta ttggactttt aatagcaggt tattttattt atggaaaaat
     3421 tgctgaacgt atttttggca ttgatcccaa cagacctact cctgctcttg ccaatcctga
     3481 tggcgtagat tatattcctt tgccatggtg gcgggtattt ttgattcaat ttttaaacat
     3541 tgctggttta ggacctatct ttggtgcaat tatgggtatt atgtttggtg aatcagcttt
     3601 tttatggatc gtatttggaa ccattttcgc tggtggagtt catgattatc tttctggcat
     3661 gatgtccatt cgtaacaatg gtgcaagtct gcctgaattg gtgggaaatg agttgggaaa
     3721 tggtatcaag caatttatga gggctttttc gctggctttg atgatactgg taggtgcagt
     3781 ttttgtttca ggtcctgcac aacttctggc aaattttact cctcatgcta ttaatgtaag
     3841 tggatggatt gtaattgtat ttacctatta tattttagct actctgttgc ccattgacaa
     3901 aattattgct aaaatttatc cattttttgg ttttgtatta ttatttatgg ctgttggaat
     3961 ttttttggct cttatactca ataaagctcc tattccagag ttcaccgaag gatttgccaa
     4021 tcttcatccc aataagttac ctatttttcc tattatgttc gtttccattg cttgcggtgc
     4081 tatttcagga tttcatgcaa cccaatctcc acttatggct cgctgcattc aaaatgaaaa
     4141 attaggacgt cgtatttttt atggcagtat ggttgccgaa ggaattgtag ccttaatttg
     4201 ggctgcagca gcttccagtt
//
LOCUS       DAOSAS010000140         4193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_13347_length_4193_cov_14.144273, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000140 DAOSAS010000000
VERSION     DAOSAS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13347_length_4193_cov_14.144273"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..999)
                     /locus_tag="PLR72_06485"
     CDS             complement(253..999)
                     /locus_tag="PLR72_06485"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOK36881.1"
                     /translation="MNDGKKIYFLADAHLGSRLVKNPREHEQKLVNWLEFVRKDAQAI
                     YLLGDIFDFWFEYKTVVPKGFVRFLGKLAELTDEGIEIHFFTGNHDIWTFGYLENEIG
                     LIVHREPCTLRLGNKQFFLAHGDGLYEDGLGFRIIKTIFHNRFAQKLFSWLPPQWGQT
                     FGYSWSKRNREKILPLENKYLGEDKENLVLFAKDYAAHHDVDFMVFGHRHIALDLQLR
                     HKKRVIILGDFIGIFSYGVFDGEDFWLEYF"
     gene            complement(1042..1356)
                     /locus_tag="PLR72_06490"
     CDS             complement(1042..1356)
                     /locus_tag="PLR72_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOK36882.1"
                     /translation="MAEFLDLEDKIVKMLRTVYDPEIPVNVYDLGLIYKIDVSDNGHV
                     TIDMTLTAPNCPISDFIFEDIRSKIESIEEVNGLDINLVFEPAWDKSMMSKEAQAELG
                     LL"
     gene            complement(1442..1834)
                     /locus_tag="PLR72_06495"
     CDS             complement(1442..1834)
                     /locus_tag="PLR72_06495"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOK36883.1"
                     /translation="MKYTYEIEMKVRNYECDFQGIVNNAVYQNYFEHTRHEFLASHYL
                     FLTDLHQQGIDPVVARIEIAYKKPLRPGDEFISKLYVKKESVKYVFVQALFRKADGVL
                     CTQAKVETVVLKDGKMLSVFEPFEKLVE"
     gene            complement(1846..2472)
                     /locus_tag="PLR72_06500"
     CDS             complement(1846..2472)
                     /locus_tag="PLR72_06500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOK36884.1"
                     /translation="MQLEDYIFAHSDKEPDYLTDIHRNTHVKLLNPRMVSGPFQGRVL
                     AMLCKMIQPQVALEIGTFTGYSALCIAEALTPNGILHTIEINEELEEIIQENFSRTSI
                     GDKVKLHIGNALEIIEKFEETFDLVYIDGDKREYLAYYEAIFPKLRSGGFILADNTLW
                     NGKVIQPTKQNDKQTLSILAFNKAIKDDERIEKIILPIRDGLTIIRKK"
     gene            2917..3156
                     /locus_tag="PLR72_06505"
     CDS             2917..3156
                     /locus_tag="PLR72_06505"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOK36885.1"
                     /translation="MQDKIITFKNYDSMVNALFEQEILRANGIECFINNEKTVELYPM
                     LSNINEGIKLIIFEKDFEKANKILGEYYQSNETNK"
     gene            3315..4037
                     /gene="recO"
                     /locus_tag="PLR72_06510"
     CDS             3315..4037
                     /gene="recO"
                     /locus_tag="PLR72_06510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOK36886.1"
                     /translation="MLTKTEGIVLHSTRYSDFASIVTIYTRQFGRNAYMVYGKGKKKS
                     IYKPAFFQPFSIVELDVCHSPKKEIQQIKDIKLSFLPQTLFFDPVKNSIALFLSEILF
                     KVLLQAETDEQLYSFLENSIKILDTMEEGTSNFHLIFLLQLTRFLGFKPNSDNPSKTY
                     FDLLNGTFLSHPPLHSHFLGPELTVDFILLLKSNYSTMANVLLNHQKRKELLENVLQY
                     YQLHISGLSNIQSLEVLEEVFT"
BASE COUNT         1434 a          687 c          611 g         1461 t
ORIGIN      
        1 aaagtgtaag ttactgctgg tgcagatatt tatttgtgcc attgctcatt agagcaaaaa
       61 aaattagtta catttgcaca aagctggaga acatttacat ccaccgagca gattttaaac
      121 tatgacaaaa aatgaaaaag tatattggta ctgacaaatg ggaaatggat tatttatttt
      181 tttttaaaac aaggtacacg caagtgcctg caatagtgag agaaatgaga tttttatctt
      241 tttgaaaatt ttctaaaaat attccaacca aaaatcttct ccatcgaaaa caccatacga
      301 aaaaattcct ataaaatcgc ccagaataat cactcttttt ttatgtctca attgtaaatc
      361 gagggcaatg tggcgatgac caaaaaccat aaagtccacg tcgtgatgag cggcataatc
      421 ttttgcaaaa agtactaaat tttctttatc ctcgcctaaa tatttgtttt caagcggaag
      481 aattttttcg cgatttcgtt tcgaccatga atagccaaac gtttgtcccc attgtggcgg
      541 caaccaacta aacaattttt gtgcaaaacg attatgaaat atcgtcttta tgatccgaaa
      601 tcctaaccca tcttcatata aaccatcacc atgagccaga aaaaactgtt tatttcccaa
      661 ccgaagtgta caaggttctc gatgcacaat caatcctatt tcgttttcca gataaccaaa
      721 agtccaaata tcatgattgc ctgtaaaaaa atgaatctca atgccttcat cagtcaattc
      781 agcaagtttt cccagaaaac gaacaaatcc tttgggaacc actgttttgt attcaaacca
      841 aaaatcaaaa atgtcaccca gtaaataaat ggcttgagca tcttttcgaa caaattccag
      901 ccaatttact aatttttgtt catgttcacg cggatttttt actaagcgag aacccaaatg
      961 agcatctgca agaaaataaa tcttttttcc atcattcatt ttcagaaaaa atacaagttc
     1021 atttacattt ttatgtcgtt ttcaaagtaa tcctaattct gcttgagctt ctttactcat
     1081 catactcttg tcccatgcgg gttcaaacac caagttgata tccaaaccat ttacttcttc
     1141 tatactttct attttagagc gaatatcttc aaatataaaa tctgaaatcg gacaatttgg
     1201 tgcagttaac gtcatatcta ttgttacatg tccattgtca gaaacatcaa ttttgtaaat
     1261 caaccccaga tcataaacat taacagggat ttccggatcg taaactgtac ggagcatctt
     1321 tacgatttta tcttccagat ctaaaaattc tgccatattt gttgcaataa ataattttca
     1381 tttcaaacac acaaaaaaca aaaaggtttt cttagtctct tatttattga cgaataacta
     1441 actattcgac caatttttca aaaggttcaa atacacttaa cattttcccg tctttcaaga
     1501 caactgtttc tacttttgcc tgagtgcaca aaactccatc agcttttctg aaaagagcct
     1561 gaacaaaaac atattttacg ctttcttttt tcacataaag ttttgaaata aattcatcgc
     1621 ctggtcgcaa aggttttttg taagcaattt ctatacgtgc caccacagga tcaattcctt
     1681 gctgatgcaa atcggtaaga aacaaataat gcgaggctaa aaattcgtgt cgtgtatgtt
     1741 caaaataatt ttggtacaca gcattgttaa caattccctg aaaatcacac tcataattgc
     1801 gtactttcat ttctatttcg taagtatatt tcattgcaat tttatttatt tctttcgtat
     1861 aatggtcaat ccgtctctaa tcggaagaat gattttttct attctttcgt cgtctttgat
     1921 agctttatta aaagcaagga tgctcaatgt ttgcttgtca ttttgtttag ttggttgaat
     1981 aactttccca ttccataaag tgttatctgc cagaataaat cctcctgaac gcaattttgg
     2041 aaatatggct tcgtaataag caagatattc acgtttgtcg ccatctatat aaacaagatc
     2101 aaaagtttct tcgaattttt ctattatttc caaagcgttt cctatgtgta atttgacttt
     2161 gtcacctatt gatgtacgag aaaaattttc ttgaataatt tcttccagct cttcattgat
     2221 ttcgatagta tgtaaaatgc cgttaggagt caatgcttct gcaatacaca gagcggaata
     2281 tcccgtgaaa gtacctattt ccaaagcaac ttgtggttga atcattttgc aaagcattgc
     2341 caatactcta ccttgaaaag gtccagaaac cattcgtgga ttgagcaatt tcacatgggt
     2401 attccgatga atatctgtta gataatcagg ttctttatcg gaatgggcaa aaatataatc
     2461 ttcaagctgc attgtttttt atgaataaaa aagcagaaag ttcatttgtt agtttctttc
     2521 tttaattttc ttggcaaaga taaagggatt ttgaagaaag aaaaaatctt ttttaaaatc
     2581 aagaaagcat attattaaaa atgaatgttt tacagtaaat ttgaaaaaca gttgctcttc
     2641 tacgaagaga aatttctccc agcttttaat cgtaagaatt ccccgatgct tgcgtcgagg
     2701 atagtcaatt ttaaggaatt tctttatttg aaaaaattta caaaaaagca agaaaaaatg
     2761 catcagatat tgggaaagtt cttttatttt tatgacttct ttttatttca aatattgaat
     2821 tgttaaaaaa caaactcttc taaagaattg tttctattta aaagatttta ctatctgctt
     2881 tgaattttgt tttattaata gtttaaaaaa tttaccatgc aagataaaat tattactttc
     2941 aaaaattatg attctatggt taatgccttg tttgaacaag aaattttacg tgctaatggt
     3001 atcgaatgtt ttattaacaa tgaaaaaaca gttgaacttt atcccatgtt gagtaatatt
     3061 aatgaaggaa ttaaattaat tatttttgaa aaagattttg aaaaagcaaa taaaatactt
     3121 ggagagtatt atcaatcaaa tgaaacaaat aaataaacat tttaaattcc aatgcactgt
     3181 aaatcaatgt cctataatct aagttgaata gaaatagcaa agaaaaaaat tacaaagaat
     3241 gaaggttatt tgaaataatt gatgatcgag gatagaaaat atattaattt tgtgttgcta
     3301 aaaatttttt gaaaatgctg accaaaacag aaggtatagt gcttcattct accagatatt
     3361 ctgattttgc ttctattgtg actatttata ctcgtcaatt tggacgaaat gcttatatgg
     3421 tttacggaaa aggtaaaaag aaatcaatct ataaacctgc cttttttcag ccattttcaa
     3481 ttgtagaatt agatgtttgc cattctccaa agaaagaaat tcaacaaata aaagatatca
     3541 aactttcctt tttgccacaa accctctttt ttgacccagt taaaaattca atagcattat
     3601 ttctttccga aattcttttt aaagtgcttt tgcaagcaga aacggatgaa caactgtatt
     3661 cgtttttgga gaattcaata aaaattttag atacaatgga agaaggaact tcaaattttc
     3721 atttaatatt tttacttcaa ttaactcgtt ttcttggatt caagcccaat agcgataatc
     3781 cttctaaaac ttactttgat ttgttaaatg gcacttttct ttcgcatcca cctcttcatt
     3841 cacacttttt gggtcctgaa ctaacagtag attttatctt attattgaaa agtaattatt
     3901 caactatggc aaatgtgctt ttaaatcatc aaaagcgaaa ggaattgtta gaaaatgttt
     3961 tgcaatatta tcaactgcat atatctggtc tttctaacat tcaatcgttg gaagtgctgg
     4021 aagaagtatt tacttaatta tcagcttatt atttattaag atgaatgaag tgtaaaaatg
     4081 atttttattc tgtttcttaa taaggtaata aggcttgatt taggggaaag tcatttctgt
     4141 taggaaggtt tttagtaaga taaggtggaa aaatgggcgt ttgttggcta aaa
//
LOCUS       DAOSAS010000141         4146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_13545_length_4146_cov_12.657297, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000141 DAOSAS010000000
VERSION     DAOSAS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13545_length_4146_cov_12.657297"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1112
                     /gene="gcvT"
                     /locus_tag="PLR72_06515"
     CDS             42..1112
                     /gene="gcvT"
                     /locus_tag="PLR72_06515"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOK36887.1"
                     /translation="MKTTPFTHFHLELGAKMQEFAGYNMPIEYSSISDEHLAVRQKVG
                     VFDVSHMGEILVKGPKALAFLQKMTTNDVASLPLGKAQYSCFTNEAGGIIDDLLVYHI
                     EPEKYLLVINAANVEKDWEWCVLNNSENAEIENISDQVAQLAVQGPLAVQVIQKLTSI
                     NLSEIPYYHFTIGKVVEVEPVLLSNTGYTGAGGFELYFSPEYADKIWNALFEAGKEFG
                     IQPIGLGARDTLRLEKGFCLHGNDIDETTTPLEAGLDWITKFLPEKNFIGQAALEKQK
                     QKGIQRKLIRFELLEKGIPRHGYDILNQHNEIIGKVTSGSFIPGDKTGIGMGYIKTDY
                     AKKNTEIYIQIRNKSLPAKIVK"
     gene            complement(1175..3469)
                     /locus_tag="PLR72_06520"
     CDS             complement(1175..3469)
                     /locus_tag="PLR72_06520"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOK36888.1"
                     /translation="MTKITREEALLYHSQGKPGKIEIVPTKPYSSQRDLSLAYSPGVA
                     EPCLEIKKSEDAVYQYTNKGNLVAVISNGTAVLGLGDIGPLAGKPVMEGKALLFKIFA
                     GIDVFDIEVKEKDPEKFIQIVKAISPTFGGINLEDIKAPECFEIEERLRQELDIPIMH
                     DDQHGTAIISAAGLLNALEIAGKKIGEVKIVINGAGAAAISCTKLYIKLGAKPENIIM
                     LDSKGIINKQRTDLTEIKKPFATDKNIHSLEEAIKGADVFLGLSVNGILSIEMVRSMN
                     ENPIIFALANPDPEISYEDAMNAREDIIFATGRSDYPNQINNVLGFPYIFRGALDIRA
                     TCINEEMKLAAVYALADLAKKPVPDVVNAAYNLKKLSFGRDYIIPKPLDPRLLTVVAP
                     AVAHAAMESGVARQKITDWEAYNIKLRELMGGYGDKMLRRFYDMARQNPKRVVFSEAN
                     HINMLKAAEMAYNEGICYPILLGNEEKIEKIANEYNIDLTGMEIINLRHDREEQRRIS
                     YAEQLANKLQRDGMTFDEAYEKMYERNYFGMMMVERGEADALITGVYTKYADSIQAAK
                     DIIGIREGSKNIAALHIMNTKKGTFFLADTLFNRHPSTETLLDIAKLTYRTVKLFAHE
                     PVMAMLSYSNFGSDKQGSPASVHEVVATLHREYPEMIVDGEMQVNFALDKELRDRKYP
                     FSKLAGKDVNTLIFPNLSSANSAYKLLLEIGLAESIGPIQMGLNKPIHILDVESSVRD
                     IVNMTTIAVIDAMLEEQKNSGKVS"
BASE COUNT         1389 a          750 c          671 g         1336 t
ORIGIN      
        1 atttattttt cagaaaaaat tcaaaatcaa aaaattcatc aatgaaaacc actccattta
       61 ctcactttca tcttgagctt ggagctaaaa tgcaagaatt tgccggttac aatatgccta
      121 ttgaatatag cagtatcagc gatgaacatc tggcggttcg tcagaaagtc ggtgtttttg
      181 atgtttcaca catgggcgaa attcttgtaa agggaccgaa agcgttagcc tttcttcaaa
      241 aaatgacaac caacgatgtt gcttctttgc ctcttggtaa agctcaatat tcctgtttta
      301 ctaacgaagc aggtggaata atagatgatt tgttggttta tcatatagag cctgaaaaat
      361 atttattggt catcaatgct gctaacgttg aaaaagattg ggaatggtgt gttttaaata
      421 acagcgaaaa tgctgaaata gaaaatattt cagatcaggt ggctcagtta gccgtacaag
      481 gtcctttagc tgtccaagtc attcaaaaat taacgtctat taatttatca gaaattcctt
      541 attatcattt tacgatagga aaagttgttg aagtagaacc agttctctta tctaatacgg
      601 gttacactgg agctggcggt tttgaactat atttctcgcc tgaatatgct gataaaatat
      661 ggaatgcact ttttgaagca gggaaagaat ttggtatcca gcctatcggg cttggtgctc
      721 gcgatacttt acgcctcgaa aaaggatttt gcctgcatgg taatgatatt gatgaaacta
      781 ccactccatt agaggcagga ctggattgga ttaccaaatt tctcccggag aaaaatttta
      841 ttggtcaagc cgcacttgaa aaacaaaaac aaaaaggtat tcaacgcaaa ttaataagat
      901 ttgagctgtt agaaaaaggt attccccgtc atggatacga tatattaaac cagcataatg
      961 aaataattgg gaaagtaact tcgggaagtt ttattccagg tgacaaaacc ggaattggaa
     1021 tgggatatat aaaaacagac tatgcgaaaa aaaatactga aatttatatt caaatcagaa
     1081 acaaaagttt acccgcaaaa attgtaaaat aaaaatcaaa aagctgcttt tctgaagaaa
     1141 aaaggcagct ttttacttag tttttcaata ttatttaact taccttccca ctattttttt
     1201 gttcttccag catggcatca attacagcaa tggttgtcat attgacaata tcacgcactg
     1261 agctttctac atccagaata tgaataggtt tattcaaacc catctgaatc ggtcctattg
     1321 attcggccag acctatttcc agcaataatt tataagcact gtttgccgaa cttaaattag
     1381 ggaaaattaa ggtatttacg tcctttcctg ctaatttgga gaaaggatat tttctatcac
     1441 gtaattcttt atccagagca aaattaacct gcatttcacc atcaacaatc atttccggat
     1501 attctctgtg caaagtggcg accacttcat gaacacttgc cgggctgcct tgcttatcgg
     1561 aaccaaaatt ggaataagat agcatcgcca ttactggttc atgagcaaaa agttttacag
     1621 ttcgataagt caattttgca atatccagta gtgtctcagt agaaggatgg cgattaaaga
     1681 gtgtatcagc caggaaaaaa gttccttttt tggtattcat aatatgaaga gctgcaatat
     1741 tttttgatcc ttcacgaatg ccaataatat ctttagctgc ctggatagaa tcagcatatt
     1801 tagtataaac gcctgtgatc aaagcatcgg cttcgcctct ttcaaccatc atcattccaa
     1861 aataattacg ttcatacatt ttttcataag cttcgtcaaa agtcatccca tcgcgttgta
     1921 gcttattggc aagttgctca gcataagaaa ttcttcgttg ttcttccctg tcatgacgca
     1981 aattgataat ttccatgcca gtaagatcta tgttatattc attagcaatt ttttcaattt
     2041 tttcttcatt gcctaatagg attggataac aaattccttc attataagcc atttcagcgg
     2101 ctttcaacat atttatatga ttggcttctg aaaacaccac tcttttggga ttttgacgag
     2161 ccatatcata aaaacgtcgc aacattttat ctccataacc acccattaat tcacgcaact
     2221 taatattata agcttcccaa tctgtaattt tttgacgtgc cactcccgat tccatagctg
     2281 catgtgctac agcaggagca actacggtta acaatcgtgg atcaagcggt ttcggaataa
     2341 tataatctct gccaaaactt aattttttca aattataagc tgcatttact acatcaggca
     2401 ctggcttttt tgctaaatca gccaaagcat atacggctgc cagtttcatt tcttcattga
     2461 tacaggttgc tcttatatcc aaagcaccac gaaaaatata cggaaatccc aatacattat
     2521 taatttgatt agggtaatcg gatcttcccg tagcaaaaat gatgtcttca cgagcattca
     2581 ttgcatcttc ataagaaatt tcagggtcag gattggcaag agcaaaaatg ataggatttt
     2641 cgttcatgga gcgaaccatt tcaatggaca aaattccatt taccgataat cccagaaaaa
     2701 catcagctcc ctttattgct tcttctaaag aatgaatatt tttatcagtg gcaaatggtt
     2761 tttttatttc tgtcaaatca gtacgttgct tgttgataat ccctttagaa tcaagcataa
     2821 ttatattttc aggttttgca cccagcttaa tatacaattt ggtacacgaa attgctgctg
     2881 ctcccgctcc attgatcacg atttttacct ctccaatttt tttcccagca atttctaatg
     2941 cattcaacaa tcctgccgca gaaatgatgg ccgtaccgtg ttggtcatcg tgcataatag
     3001 gaatatctaa ttcctgtctt aatctctctt caatttcaaa acattcaggt gctttaatgt
     3061 cttctaaatt gataccacca aaagtaggcg aaatagcttt tacaatctga ataaattttt
     3121 ctggatcttt ttcctttact tcaatatcaa aaacatcaat accagcaaaa attttaaaca
     3181 ataatgcttt cccctccatc acaggtttcc ctgccagagg accaatatct cccaatccaa
     3241 gaacagccgt tccattggaa ataactgcca ccaaatttcc cttattggtg tattggtaaa
     3301 cagcatcttc actcttttta atttcaagac acggttctgc aactcccggc gaatacgcta
     3361 gtgacaaatc acgctgcgag ctgtaaggtt tggtaggaac aatttcaatt ttacccggct
     3421 ttccttgtga atgataaaga agggcttctt ctctggttat tttagtcata taataaatat
     3481 aaattgcgtt taaattaaaa agtgaaagag aaataaagac ttgtttagat aattgtagca
     3541 aaattacaat tttttataac aaaatacaat tttccatcgc cccattaaaa acaagtagag
     3601 tcaactagca agtgcgaaat tatataaaat gtttgaaaca gattttcctt tctaaatttt
     3661 gtaaaaaatg aactaaaaaa cagtaccaat aagcttttat gaacattcca gtaaatttaa
     3721 ggtacgcaag aaaaatacta ttttctcctt ctaacaaagt tattttgttg aatttttttg
     3781 tatgtttgta aaaaataaaa taatgttcaa tatgtgataa ataactaata attatactgt
     3841 aagcaagaaa ttattttaaa aaaatatatt aaaacagata ataatcaatt tgttacgaat
     3901 aaaatatacc acttttgaaa agaaaatccg atataagaga aaaagggagg atttaaaaat
     3961 agaaccttca aatcttttga aagatgttga agatacatta gcagaacaat taaaaaatct
     4021 ggataaaaaa acagtaaaat tagtctgttt atatcattgg attaaggaag tgctttgata
     4081 gcggtttggt ataaatgttt catctttgta actttttaag gggaagagac caaagtaaag
     4141 cattta
//
LOCUS       DAOSAS010000142         4073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_13891_length_4073_cov_12.413639, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000142 DAOSAS010000000
VERSION     DAOSAS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13891_length_4073_cov_12.413639"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1412)
                     /locus_tag="PLR72_06525"
     CDS             complement(120..1412)
                     /locus_tag="PLR72_06525"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK36889.1"
                     /translation="MKTSNIPFLQGLLVFLFLMGFSCQGWASEKKQNYQQQAQDYLEY
                     KGKIIDRSNKKVLPFAHLRVEGTNISTVSNAEGEFLIKIPEELKDKKLIISYIGYKNA
                     EFPFYQLNTENIRIALEPFVIELPEINIISRDANELIKSVFEKRNVNYPNQKIFMTAF
                     YRESIKKNRSYVSLAEAIVDILKQPYTSYLSDEAQFYKSRKSTDYKKLDTLVFKLMGG
                     PYNSLYLDIMKYPEYLFMDDMMTNYEFTFDHVTYTDHQMIYIVDFKQQPYIKEPLYYG
                     KLYIDAETLALQSAIFNLNLDDKERAASLFIRKKPFNARVFATKASYRIDYIKRGEKW
                     YYNYSRIELNFKIDWKKRLFNSNYYSIIEMAITDWEPFTEEKKIKNKIKHTVILTDEA
                     SGFSDPAFWGEYNVIEPEQPIETAIKKIQKQLQKRTQD"
     gene            1963..3279
                     /gene="mtaB"
                     /locus_tag="PLR72_06530"
     CDS             1963..3279
                     /gene="mtaB"
                     /locus_tag="PLR72_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOK36890.1"
                     /translation="MTCPSLLPPKKVAFFTLGCKLNFAETSHIGKQLMEAGYTKARSG
                     EEADICIVNTCTVTDAADRKCRQAIHRLHREYPHAIMIVTGCYAQLKPQEIAAIEGVN
                     LVLGSHEKFNILEYLQTFENQSNSQIFHSKQQELKTFHPSCSYEDRTRYFLKIQDGCD
                     YYCTYCTIPFARGRSRNGSISEIITLAEKAISEGAKEIVLTGVNVGDFGKSTGESFFN
                     LLKALDNLPYGIRFRISSVEPDLLSDEIIKWIAQSRHFMPHFHIPLQSGSNEILRIMR
                     RRYNTELFASKIDTIKKWMPDAFIGVDVIVGVRGETKKLFEETKNFISSLNISQLHVF
                     TYSERQSTKMLEIKEIVPLSERKKRSEILHQLSDEKVKIFYEQQIGKPAQVLWENRRK
                     GEKMLGFTQNYVKVEKNYDKKSINTIQNIILSSWNKEHTALQAIAL"
BASE COUNT         1492 a          589 c          662 g         1330 t
ORIGIN      
        1 taaattttcg taagcaccac caccaaaaat tgttgattgt tgaatttgat ttcatgtcca
       61 ctttttattt atcgctgcaa agttgaaaaa ttattttgaa ttgtgcaaca aaaattgaat
      121 taatcttgtg ttcttttttg cagttgtttt tgaattttct taatagctgt ttcaataggt
      181 tgttctggtt caattacatt atattcgccc caaaaagcag gatctgaaaa acctgaagct
      241 tcatcggtga gaattactgt gtgcttaatt ttatttttaa tttttttctc ttcggtaaaa
      301 ggttcccaat cagtaatagc catttcaata atagaataat aatttgaatt aaatagtctt
      361 tttttccaat caattttaaa attcaattca attcggctat aattataata ccatttttct
      421 cctcttttaa tataatcaat tcgatacgaa gctttagtag caaagactct ggcattgaaa
      481 ggcttttttc taataaataa agaagctgct cgttctttat cgtcaaggtt taaattaaaa
      541 attgcactct gtaaagctaa agtttcggca tcaatatata atttcccata ataaagaggc
      601 tcttttatgt aaggttgttg tttgaaatca acgatataaa tcatttgatg atcagtataa
      661 gtaacatgat caaaagtaaa ttcataattg gtcatcatat catccataaa gagatattcc
      721 ggatatttca ttatatccaa atacaaagaa ttataaggtc cccccattaa tttaaaaaca
      781 agcgtgtcta attttttgta atctgtgctt tttctacttt tgtaaaactg tgcctcatca
      841 gacaaatagg aagtataagg ttgttttaaa atatccacaa tagcttcagc caacgaaaca
      901 taactacggt tttttttgat agattcacgg taaaaagctg tcatgaaaat tttttgattc
      961 ggatagttta cattgcgttt ttcaaaaact gatttaatta attcattcgc atctcttgaa
     1021 ataatattaa tttcaggcaa ttctattacg aacggttcca aagcaatacg tatattttct
     1081 gtattcaatt gataaaaagg aaattcagca tttttatagc caatataact aatgattaat
     1141 tttttgtctt taagttcttc cggaattttt atcaaaaatt ctccttctgc atttgaaacg
     1201 gtagaaatat tagtcccttc tactctgaga tgggcaaacg gcaaaacttt tttattgcta
     1261 cggtctataa ttttcccttt atattcaagg taatcctgag cttgttgttg atagttttgt
     1321 tttttctctg atgcccatcc ttgacaagag aaacccatta aaaaaaggaa aaccaacaag
     1381 ccctgaagga aaggaatgtt agaagttttc ataagagtta tgattagaaa tttttcataa
     1441 aaaataagta tagataaaac gaaaaattca actttttaag aaaattcttt tatttttaaa
     1501 aataacaaat aattatgata attagttggt ttttttaaaa ttatttttcg agtgaggtag
     1561 ctgatagtaa attgttctca taaatgaaaa aacatataaa aagagtttaa agtttgagag
     1621 atacaagata cgggtttcga ggggcgaggt gcagatttgt gagttctgaa ttctgaatta
     1681 tatatttagt tgataatcaa tgatttcaat tagagacatt tcataaaacg tctcctgtaa
     1741 aatagaaggg caaaaaatct cagtgttttt cttttaattt taattttaat ttcaatttta
     1801 atttcaattt caatttcgat taaaacttgg tatgaataaa aaccaacaaa tgagtaggta
     1861 ttttgttaat gtattgaatt agaagtcttc ttgaagaaga aagtgaaaaa cataaaattc
     1921 ataagatttt tcattaattt tgtacataat tttttataaa aaatgacatg cccttcttta
     1981 ttacccccta aaaaagtagc ttttttcaca ttgggttgca agttgaattt tgctgaaact
     2041 tctcacattg gcaaacaatt aatggaagct ggttatacca aagctcgttc aggagaagaa
     2101 gcagacattt gtattgtgaa tacttgtacc gttaccgatg ctgctgatcg aaaatgtcgt
     2161 caagcaattc atcggcttca ccgtgaatac ccacatgcta ttatgattgt tacaggttgt
     2221 tatgctcaac tcaaaccaca agaaattgct gccatcgaag gagtaaattt ggttttgggg
     2281 agccacgaga aattcaatat tctggaatat ttacaaacct ttgaaaatca aagtaattca
     2341 caaatatttc attcaaaaca gcaagagtta aaaacttttc atccttcctg ttcatacgaa
     2401 gatcgaacac gttattttct taaaattcaa gatggctgtg attattactg tacttattgc
     2461 accatacctt ttgcacgtgg cagaagtaga aatggaagta tttcagaaat tataacactt
     2521 gctgaaaaag ctatttcaga aggagctaaa gagattgttc tgacgggtgt gaatgttgga
     2581 gattttggca aatctacagg tgaaagtttt tttaatctat tgaaagcttt ggacaatcta
     2641 ccttacggga tccgatttag aatttcttct gttgagcctg acttactttc agatgagata
     2701 ataaagtgga tagctcaaag tcgtcatttt atgccacatt ttcatattcc gctgcaatcg
     2761 ggaagtaatg aaattcttag aatcatgaga agacgttata atactgaact ctttgcttcg
     2821 aaaatagaca ccattaaaaa atggatgcct gatgctttta ttggcgtgga tgttatagta
     2881 ggagtacgtg gagagacaaa aaaattgttt gaagaaacaa aaaattttat ttcatcacta
     2941 aatatctcac agttgcatgt gtttacttat tcagaacgac agagtacaaa aatgttggaa
     3001 ataaaagaaa tagttccctt atcagaacga aaaaagcgaa gtgaaatact tcatcaattg
     3061 tctgatgaaa aagtcaaaat tttttatgag caacaaatcg ggaaacctgc acaagtatta
     3121 tgggaaaacc gacggaaagg tgaaaaaatg ttgggcttta ctcaaaatta cgtgaaagta
     3181 gaaaagaact atgataaaaa aagcataaat accattcaaa atataatttt aagcagttgg
     3241 aacaaggaac atacagcttt acaagccata gcactttaac atcaagtagc tttcaaaaat
     3301 aaaaaataat ttaaaaatag ctgggaatca ggagtttaga attcacaagg ggcgatgtac
     3361 gaggatttag ttgtaatcag tagtttacga gtttcaagtt tcgagtagag acctttagtt
     3421 tgcaagttgt ccgaatgcac aaataacaaa aatctcagcc attcattttc atagagttcc
     3481 actgagaaaa atctcaaaaa taagcgaaat agtaaggcat ttttattttg tggaaggtaa
     3541 ttgggcaaaa cgaaatcgcc gaaaaaatta aaaaatactt tttgagacaa aatagtaaat
     3601 gggatggaca aaaccacaaa tgcaattact tgattaattg cgatcttaaa aaatgttata
     3661 gagtaagagc ttcaagaaac caaaaacgag ctataaatac ccatcagaaa aacataataa
     3721 acaaaataag ttgtaatact ttactttaac aagcgatagt acaagaaaat atattaccta
     3781 acaaaaggat tttacaaaaa ttaaaagttt gtttgataat ttataaaaat tttttatttt
     3841 tgaggttgaa acaaaaaaac tgttgaatta acgaagtgga caatatatag acggcaacaa
     3901 attatgcgat agcacaatcg ccaacatctt gaaaatatgg gcaagaaagt aaacaaaaac
     3961 gagaacaaac aagaagaacc gttagaaaaa caatcgctcg gcaagctcga ggcaacgctt
     4021 tggaaggctg cagacaaact gagaaagaat attgacgccg ccgaatataa aca
//
LOCUS       DAOSAS010000143         3972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_14352_length_3972_cov_13.298443, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000143 DAOSAS010000000
VERSION     DAOSAS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14352_length_3972_cov_13.298443"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..887
                     /gene="pyrH"
                     /locus_tag="PLR72_06535"
     CDS             171..887
                     /gene="pyrH"
                     /locus_tag="PLR72_06535"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOK36891.1"
                     /translation="MLLYKRILLKLSGESLMGTKQYGLDESRLQEYATQIAEISSMGV
                     EVGIVIGGGNIFRGINGESKGFDRVLGDQMGMLATVINSLALCSALQAIGANSRVLTA
                     IRMEPIGEFYSKQKAVSCLQQGEIVIISGGTGNPFFTTDTASSLRAIEIGADVMLKGT
                     RVDGVYTADPEKDANAVKFEEITYDEIYRRSLKVMDLTATTLCKENNMPIYVFNMDKA
                     GNLKKVVLGEKIGTLVKNNF"
     gene            993..1556
                     /gene="frr"
                     /locus_tag="PLR72_06540"
     CDS             993..1556
                     /gene="frr"
                     /locus_tag="PLR72_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOK36892.1"
                     /translation="MTEEIKPILDEAKKKMEETIRFLESSLARIRAGKANPHILDVVK
                     VEYYGNHVPLSNVASITTPDAKTILIQPWEKTMIKAIEKAILNANIGFTPTDNGEVIR
                     LTLPPMTEERRKELVKQVRIEAEEARITIRNARRDVIEQIKKKVKEGLSEDIEAVGTE
                     EAQKLHDNYVKKIDDLLAAKEKEIMTV"
     gene            1719..2657
                     /gene="rsgA"
                     /locus_tag="PLR72_06545"
     CDS             1719..2657
                     /gene="rsgA"
                     /locus_tag="PLR72_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOK36893.1"
                     /translation="MKGLVVKNTGSWYEVKTNDGKRINCKIKGNFRLKDIRSTNPIAV
                     GDWVEFEINKEGKGIIYNICERKNYIIRRSSNFSKEVSILAANLDAAALVVTVNYPET
                     STVFIDRFLAVAEAYSVPACLIFNKIDLYSEEEKEYLDAITYLYESIGYPVFQVSALY
                     AINLAPLTDFLKDKITLFAGNSGVGKSALINALSPTANAKTSEISSYHRKGVHTTTFS
                     EMYELPFGGHVIDIPGIKGFGLIDMKEDEIDHYFKEIFAISSQCRFDNCLHLNEPGCA
                     VIQAVENNTISLSRYQSYLNILEDVHEGKYRKDEYL"
     gene            3700..>3972
                     /locus_tag="PLR72_06550"
     CDS             3700..>3972
                     /locus_tag="PLR72_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36894.1"
                     /translation="MAIEKAFRISKTDLRIRPIYHRIRNRIEARICIAFTAYCILKEL
                     ERVFTQREVSYIAEKGSGTDSKYVSNHLYFARIKTNKIQTSQHGQRT"
BASE COUNT         1423 a          564 c          749 g         1236 t
ORIGIN      
        1 gagtttttca aacatttttt ataatttcgc acttgctggt tgactctact ttattttttc
       61 tttttctcag ttgtttgtaa ataccattct atttatttat ttttgtctga taatcttttc
      121 ttttttgtag atttataaga agaagctatc gaaaataaaa aatattttat atgctgcttt
      181 ataaacgaat tttgttgaaa ctgagcggtg aatctttgat gggaacaaag caatacggtc
      241 ttgatgaaag tcggttgcag gaatatgcaa ctcaaatcgc tgaaatttcg tcaatggggg
      301 tagaagtagg aatagtaatt ggcggaggga atatttttcg tggaataaat ggtgaatcaa
      361 aaggttttga tcgtgtttta ggtgaccaga tgggaatgtt ggcaacggtc attaatagct
      421 tggcactttg ttcggctttg caagcgattg gggcaaactc acgggtgctg acagctataa
      481 ggatggaacc gataggagaa ttttatagta aacaaaaagc tgtttcttgc cttcaacagg
      541 gagaaattgt aattatttca ggaggtacag ggaatccatt ttttaccacc gatacagctt
      601 cctcattacg agcaatagag ataggagctg atgttatgtt aaaaggaaca cgtgtagatg
      661 gtgtatatac tgctgatcca gaaaaagatg ccaatgctgt aaaatttgaa gaaattactt
      721 acgatgaaat ttatcgccga agtttgaaag taatggattt gacagcaaca accctttgta
      781 aggaaaataa catgcctatt tatgtcttta atatggataa agctggcaat ttgaaaaaag
      841 tagttttggg tgaaaaaata ggcactttag taaaaaataa tttttaagaa aacttggagg
      901 atatttttaa ttatttcatt tataaataat aaacttaatt aaccacacta atttctgtta
      961 tgcagaatta agtgataaaa attaccatta tgatgacaga agagattaaa ccaattctgg
     1021 atgaggcaaa aaagaaaatg gaagaaacca ttcgtttttt agaaagttca ttagcgcgta
     1081 ttcgtgcagg gaaagctaat cctcacattt tagatgtggt aaaagtggaa tattatggga
     1141 atcatgtacc attaagtaat gtagcttcta ttactacacc tgatgctaaa actattttga
     1201 ttcaaccgtg ggaaaaaaca atgataaaag ctatcgaaaa agcaatttta aatgccaata
     1261 taggttttac tccaactgat aacggagaag ttattcgttt aactttaccg ccaatgacgg
     1321 aagagcgtcg caaagaatta gtaaaacaag ttcgcataga agcagaagaa gccagaataa
     1381 ctattcgtaa tgctcgtcga gatgtgattg agcaaattaa gaaaaaagta aaagaaggtt
     1441 tatcagaaga tattgaagct gttggtacag aagaagccca aaagcttcat gataactatg
     1501 ttaaaaaaat agatgatttg cttgctgcca aagaaaaaga aattatgaca gtttagatat
     1561 tggtgcggat attaaggttc aattgataga attcctcctt ccttaaaaga attttatcaa
     1621 ttgatttttt atttttccaa aacaaaaaat tcctttctat tttaattttt tcatttattg
     1681 ctaaatgcct acctaataaa ttttcaattg aattattcat gaaaggtctt gtagtaaaaa
     1741 atacagggag ttggtatgag gtgaaaacta acgatggtaa acgaattaac tgtaaaatta
     1801 aaggtaattt tcgtttaaaa gatattcgta gcaccaatcc tattgcagtg ggtgattggg
     1861 tagaattcga aataaacaaa gaaggtaaag gaataattta taatatctgt gaaaggaaaa
     1921 attatattat tcgtcgttcg tccaattttt caaaagaagt tagcatttta gctgctaatc
     1981 tggatgcagc agctttagtg gtgacagtaa attatcctga aacttcaacc gtttttattg
     2041 atcgtttttt agcagtagct gaagcttaca gtgttccagc ttgcctgata tttaacaaaa
     2101 ttgatttata ttctgaggaa gaaaaagaat atcttgatgc tattacttat ttgtatgaaa
     2161 gcattggcta tcctgttttt caagtttcgg ctttatacgc catcaattta gctcccttaa
     2221 cagatttctt aaaggacaaa attactcttt ttgctggtaa ttcaggggta gggaaatcgg
     2281 cacttattaa tgcactttca cccacagcta atgcgaaaac gtctgaaata tcttcttacc
     2341 atcggaaagg agttcatact actacttttt cagaaatgta tgaattgcct tttggtggtc
     2401 acgtcattga tattcccggc ataaaaggat tcggattgat tgatatgaaa gaagatgaaa
     2461 ttgaccatta ttttaaagaa atttttgcaa tatcttctca atgcagattt gataattgct
     2521 tgcatcttaa tgaaccaggt tgtgcagtta ttcaagctgt agaaaataat actattagtt
     2581 tgtcaaggta tcaaagttat ctcaatatat tagaagatgt tcacgaagga aaatatagaa
     2641 aagatgaata tctttaattt ctggtaataa ttaatagaga aaattgacat aatggctaaa
     2701 attaggctaa atttttctac aagtttgata aataccaact ttgtgttctt ggaaagaata
     2761 tgaataaaaa aatttgcttt gtgacttaaa ttcggaaatg ataaattgct ctccagtagg
     2821 gttatgtgtt tttatgtaaa gtttaaaaaa ttaattgcac aaatgcctag tactttttca
     2881 atggagatta aaaaggttat aaatattctt gtttttccgc actttttcga gacatctata
     2941 acacattgat aatcaataat aattttataa tttggggtgt tccaaaatta tttataggtt
     3001 tgtaagaaaa acaaaatcga tctggaaatc aatctgtaca agtaatacag aagtttcgaa
     3061 gaaagtataa agtagtgaaa atcattggtc gtaccaccac gcagcacgaa attgagagat
     3121 tggaataatt ggcaagagaa gaaataagtc atttaagttg aaatcagtta aagttttttg
     3181 actgcaaaag taatgaaatt attgagcaag ccttttctct gcttgacaat agtagtattc
     3241 gcactgttga accagagttg atctaaggaa atatctacaa aggctataca ctgatccctt
     3301 ttctggagaa aatgagccgc aagtttcatt gaagaacgaa aacgaaagga tcaaagagaa
     3361 aatacttgcc atagaattta ccgatggaat gacggtgagt atgaaaaaat cggacaatac
     3421 tcgattggta gtaaactatt caactgccag agccaagaag gactattaca accgcaaacg
     3481 gagtttagcc aggcttgaaa agcaaatcag atcgggtaaa ttgaccaaat caaatatcaa
     3541 caacagagga tataacaaat acctcaagtt gagcgatgaa atcagtgtag aaattgatta
     3601 cgaaaagtac gataatgacg gacgatggga cggcatcaaa ggttatctga ccaataccag
     3661 gttgtcggat gaggaagcaa tggaaaatta caagaatctt tggcaattga aaaagctttc
     3721 cgcatatcaa agacagattt acgcatccgt cccatttatc acaggatacg caatcgcata
     3781 gaagctcgta tttgcattgc ttttacagct tactgcatac tcaaagaact ggaaagagtt
     3841 tttacacaaa gagaagtcag ctatatcgct gaaaagggct caggaactga ctcaaaatat
     3901 gtatcaaatc acttatactt tgcccgaatc aaaacaaaca aaatccaaac ttctcaacat
     3961 ggacaaagaa ca
//
LOCUS       DAOSAS010000144         3954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_14420_length_3954_cov_12.038471, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000144 DAOSAS010000000
VERSION     DAOSAS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14420_length_3954_cov_12.038471"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1409
                     /gene="der"
                     /locus_tag="PLR72_06555"
     CDS             96..1409
                     /gene="der"
                     /locus_tag="PLR72_06555"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOK36895.1"
                     /translation="MGNIVAIVGRPNVGKSTLFNRFTSTRRAIVSSISGTTRDRQYGK
                     CEWCGTEFSLIDTGGWVVNTSDVFEEEIKRQILLAIEECDVVLFLVDVQSGITDYDLE
                     VAKILRRSDKPVILVANKGDTFEWQYQAAEFYRLGLGDPFLISAINGLGTGDLLDKVI
                     SYFPSKKEMETEENLPRFAVVGRPNVGKSSLINAFLGEERNIVTDIAGATRDSIYTRY
                     NKFGYDFYLVDTAGIRKKTKVNEDVEYYSIVRSIRAIENSDVCILMIDATVGIEGQDL
                     SILSLIQKNKKGLVVCVNKWDLIEDKDSRSVKEYEATIRERFSPFTDFPIIFASALTK
                     QRIFKVIESAMKVYENKNRKIPTAQLNDFLLPLIQNYPPPAWKGKYIKIKYVTQLPNT
                     QIPTFLFFANLPQYVKEPYRRFLENKIRSQWNFTGTPIQLFIRQK"
     gene            complement(1445..2911)
                     /locus_tag="PLR72_06560"
     CDS             complement(1445..2911)
                     /locus_tag="PLR72_06560"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HOK36896.1"
                     /translation="MLINILSSITAYEPNFATYHLLIKIKLFNRTMKIKSIFLVSLFI
                     ILFSSCGDDLSEIGINIQPSKDFISVYTDTFHLSSQNVFVDYIYSRPDSFLLGTFYDQ
                     KYGITQADILAQVNCPVGFTYPEGSVPDSANIVLRYLTWFGDNYSPVEINIYQMNKAT
                     FSYSQPYPSNIDPADYTDKTIHLGKRIVTAKDASGSKIDTMMIRFKLSDDFVRWFYPS
                     SNKYTSQQEFLEKFKGIYITPNFGTATMFYINQIDLYYYYHYTYVRKALDGITDSTVT
                     VNNVITFPANEEVRQVNRFMHPNREQIIKQREEVNYISSPANVFTKVTIPLKKIKQKL
                     DNNINDKYLSINSAVLQVDATEVEDTTLAQPLVKYLLLIKESAMDRFFKNNELPSDTC
                     SILATYSTTKNSDNTYNYYYTFNLATLLTNELKTAKTNAAELPDQLSFLLVPVRIKYN
                     SSGSVTEVKHQFLMSAVTICSGKHPSKPMKMNLVYSGF"
     gene            complement(2874..3686)
                     /locus_tag="PLR72_06565"
     CDS             complement(2874..3686)
                     /locus_tag="PLR72_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOK36897.1"
                     /translation="MNQVSKVLYISQEIYPYLPESEMAMMGRYLPQEVQEKGLETRTF
                     MPKFGSINERRNQLHEVIRLSGMNLIIDDTDHPLIIKVASIQPARLQIYFIDNEDYFH
                     RKYTVANENNEEYDDNDERTIFFARGVLETIKKLRWTPDVIHCQGWMSALVPLYIKKA
                     YNDDPFFKHSKVVYAVFNDEFKKPFNSNFGEKLMIDGIQRKHIASHLKNQPIDYVALN
                     KLAIDYSDGVIQASSTINEAVLEYILSTKTNFLAFESNDDYADKYIKFYNSL"
BASE COUNT         1339 a          593 c          694 g         1328 t
ORIGIN      
        1 cttcaattct aattgtaaga cagcataaat tgaaaattat ctcgtaactt tgtatcaaat
       61 tttgtttaga aaaagttaat gagaaaaaat agaaaatggg gaatatcgta gcaattgttg
      121 gacgaccaaa tgtggggaaa tcaactctgt ttaatcgatt taccagcacc cgccgagcca
      181 tcgtaagttc catatcagga actacgcgcg accgtcaata tggaaaatgt gaatggtgtg
      241 gaactgaatt ttctttgatc gatacaggtg gttgggtagt aaatacttct gatgtgttcg
      301 aagaagaaat taaacgtcaa attttgctgg caatagaaga gtgtgatgtg gttctttttt
      361 tagttgatgt tcaaagtgga ataacagatt atgatctgga agtggcgaaa attttacgac
      421 gttccgataa gcctgtcatt ttggttgcca ataaaggtga cactttcgaa tggcaatatc
      481 aggcagcaga attttatcgt ttaggattag gtgatccttt tttgatttcc gctatcaatg
      541 gattaggtac aggtgatttg ctggataaag ttatttctta ttttccttca aaaaaagaaa
      601 tggaaacaga agaaaacttg ccacgttttg cagtagtagg tcgtcctaat gttggtaaat
      661 catcattgat aaatgcattc ttgggagaag agcggaatat tgttaccgat attgctggtg
      721 ctactcgcga ttctatttat actcgttata ataaatttgg ttacgatttt tatttggttg
      781 atactgctgg catccgaaaa aagactaaag taaatgaaga tgtggagtat tattccattg
      841 ttcgttctat tcgtgccatt gaaaacagcg atgtttgcat tttaatgatt gatgctaccg
      901 ttggtattga aggacaggat ttaagcattt tgtcattgat tcagaaaaat aagaaaggat
      961 tggtggtttg tgtcaataaa tgggatttga tcgaggataa agatagccgt tccgtaaaag
     1021 aatatgaagc aaccatcaga gaacgttttt ctccttttac agattttcct attatttttg
     1081 cttcagcttt gacaaaacag cgaattttca aagtgattga atcggctatg aaagtttatg
     1141 agaacaaaaa cagaaaaatt cctactgcac aattgaatga ttttttgctg ccattgattc
     1201 aaaattatcc tccaccagca tggaaaggaa aatatattaa gataaaatat gtgacacaat
     1261 tacctaatac tcaaatacct acttttctct tttttgccaa tttaccccaa tatgtaaaag
     1321 aaccttatcg tcgttttctg gaaaataaaa ttcgttcgca gtggaatttt acaggaacac
     1381 ctattcaatt atttatcagg caaaaataaa gtttagaata aactaaaaaa ccctttagaa
     1441 tttatcaaaa tccactataa actaaattca ttttcatcgg tttggaagga tgcttaccac
     1501 tgcaaatagt aacagcgctc attaagaatt gatgttttac ttcggtgaca gaaccactgc
     1561 tattgtattt tatcctaaca gggaccaata gaaaacttaa ttggtcagga agttccgcag
     1621 cgtttgtttt agcagttttc aattcattgg tgagcaaagt agcaagatta aatgtataat
     1681 aataattata agtgttatca ctattctttg tagtagaata ggtagctaaa atggaacaag
     1741 tatcagaagg taattcatta tttttaaaaa atctatccat tgctgattct ttgattaaca
     1801 acaaatattt tacaagcggt tgtgccagtg tagtatcttc cacttctgtt gcatcaactt
     1861 gaaggacagc actgttaata gataagtatt tatcgttgat attgttgtct aatttttgtt
     1921 tgattttttt taatggtata gtaactttgg taaacacatt agctggcgaa gatatataat
     1981 ttacctcttc tctttgtttg ataatttgtt cccgatttgg atgcataaaa cggtttactt
     2041 gtcttacttc ttcgtttgca ggaaaagtaa taacattgtt aacagttact gttgagtcag
     2101 taataccatc cagagctttg cgaacgtaag tataatgata gtaataatac aagtcgattt
     2161 gattaatgta aaacattgtt gctgtcccaa aattgggagt aatatatata cctttgaatt
     2221 tttccagaaa ctcttgctga ctcgtgtatt tatttgaaga cggataaaac catctaacaa
     2281 aatcgtccga cagcttaaag cggatcatca tagtatctat tttcgaacca ctggcatctt
     2341 tggctgttac aattctttta cctaaatgta tggttttatc ggtataatca gcagggtcta
     2401 tattggaagg ataaggttga gaataagaaa aagttgcctt attcatctga taaatattta
     2461 tttctactgg tgaataatta tcgccgaacc atgtgaggta tcggagaaca atattagctg
     2521 aatcgggaac agaaccttct ggataggtaa aaccgacagg acaatttact tgagccaata
     2581 tatcggcttg agttattcca tatttctggt cataaaatgt tccaagaaga aacgaatcag
     2641 gacgggaata aatgtaatct acaaaaacat tttgagagct taaatggaaa gtatccgtat
     2701 aaacactgat aaaatctttt gatggctgaa tatttatacc tatttcactt aaatcatcac
     2761 cacaggaaga aaataaaatt ataaaaagag aaactaagaa aatacttttg atcttcatag
     2821 tgcgattgaa taatttgatt tttattaaca aatgataagt ggcaaaatta ggttcatagg
     2881 ctgttataga acttaatata tttatcagca tagtcatcgt ttgactcaaa tgcaaggaaa
     2941 tttgttttgg tagacaaaat atattccaaa acagcctcat tgatggtaga gcttgcctga
     3001 ataactccat ccgaataatc tattgccaat ttattgagag cgacataatc aatgggttga
     3061 ttttttaaat gagaggcaat atgttttctt tgaataccat caatcattaa cttttcacca
     3121 aaattactat tgaaaggttt tttgaattca tcattaaaaa ctgcataaac tacttttgag
     3181 tgcttaaaaa atggatcatc attatatgct ttttttatat acaatggaac gagtgcactc
     3241 atccaaccct gacaatggat aacatcaggt gtccaccgta gtttttttat tgtttccagt
     3301 acaccacgtg caaaaaaaat agttctctca tcattatcat catattcttc gttattttca
     3361 ttggcaacgg tatatttcct atgaaaataa tcttcattat caataaaata gatttgtaag
     3421 cgggcaggct gaatagaagc aactttgata ataagtggat gatcggtatc gtcaataatc
     3481 aaattcattc ctgaaagtcg tattacttcg tgaagttgat tacgccgctc attgatagaa
     3541 ccaaatttag gcataaatgt gcgagtctcc aaaccttttt cttgaacttc ttgtggaagg
     3601 tatcttccca tcattgccat ttcagactca ggtagataag gataaatttc ctgtgaaatg
     3661 tacaatactt ttgatacttg attcattatt cccaactaaa ataattcatt tattatgcaa
     3721 agatacaaaa aaaatcagac atttgcactt gaagcgtctt aaaataaata actaatgagt
     3781 gttaaatagt agaataaaac ttcaattttt gactctgaaa aattttttat gtaaaataag
     3841 cgactggatt tttttaaaat tatcatcaaa taattttgtt ccaatttatt gtaaaacagg
     3901 aacttcggag aggaagaaat tcgatttgtt tacaaaaaag aaaataagca aaga
//
LOCUS       DAOSAS010000145         3945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_14476_length_3945_cov_13.700514, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000145 DAOSAS010000000
VERSION     DAOSAS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14476_length_3945_cov_13.700514"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1965
                     /locus_tag="PLR72_06570"
     CDS             <1..1965
                     /locus_tag="PLR72_06570"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOK36898.1"
                     /translation="ETILGDTALCIHPDDAKNAHLKGKRVIVPMVNRSVPVIEDEYVD
                     MEFGTGCLKVTPAHDVNDYMLGEKHHLPAIDIFNDDGTLNENGTPYTGMDRFEVRKQI
                     EKDLQSAGLLEKVEPYVNKVGFSERTDVPIEPKLSTQWFLKMKEMAKPALEAVINDEI
                     KLYPPKFKNTYRHWMENVKDWCISRQLWWGHRIPAYYLPDGSFVVAETKEEAYQKALK
                     KVDYPLTIDDLKQDEDCLDTWFSSWLWPISVFDGILHPDNPDIQYYYPTDDLVTAPEI
                     LFFWVARMIMAGYEYRGEKPFKNVYLTGIVRDKLGRKMSKSLGNSPDPLDLIQKYGAD
                     AVRMGMLLCSPAGNDLLFDESLCEQGRNFNNKIWNAFRLIKGWTIVEIEQPMASAKAV
                     KWFESQLNKSLAEIDDLFAKYRLSEALMVAYKLFWDEFASWFLEIIKPAYQEPIDKIS
                     YEAAIHFFETLLKILHPFIPFITEELWQNLTERKEGESIMIQLFPQAGKIDETLLENF
                     ESTKQIIANIRAIRLQKNIPNKKPLKLQIVGKSHPYFDSIIEKLGNLDAIETVSEKSA
                     EAASFLVGTTEFAIPLAGFINVEEEIKKIQKEIDYLEGFLASVTKKLNNQNFLSHAKP
                     EIVESEKKKQSDTEKKLSSLRETLAGLMNS"
     gene            complement(2016..2453)
                     /gene="ybeY"
                     /locus_tag="PLR72_06575"
     CDS             complement(2016..2453)
                     /gene="ybeY"
                     /locus_tag="PLR72_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOK36899.1"
                     /translation="MMIQYISEGVKVPQIKKQKINRWIKAVAADYDKKIGDIAYIFCN
                     ENRILEINQKYLQHDYYTDIITFDDSERNLISGDIFICIDTVKSNAEKFQTDFMQELY
                     RVMIHGILHLCGQDDKTSEQKEEMTFEENKALSQLKEILSPKI"
     gene            complement(2610..3803)
                     /locus_tag="PLR72_06580"
     CDS             complement(2610..3803)
                     /locus_tag="PLR72_06580"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOK36900.1"
                     /translation="MINLSKRLAALSPSETLAMSQKSNELKAKGIDVINLSVGEPDFF
                     TPHHIKLAAKQAIDNNFSFYPPVPGFLELRKAICAKLKSENHLEYSPDQIICSTGAKQ
                     AICNVLLSIINPGDEVIIPAPYWVSYPEMVKLAEGKSVGVFADIEQDFKITAQQLETA
                     ITPKTKAFIICSPSNPTGSIYSKEELKQFAEVLEKYPQIIILSDEIYEHINYTGKHES
                     IAQFETIRDRVVIINGVSKSYAMTGWRIGWIAGPKWIADACNKLQGQYTSGACAVAQK
                     AAEAAYIGDQTCVREMCEAFERRKNLVVELIRQIPGLKVNDPQGAFYVFPDCSAYIGK
                     SYQGKTINDSAELAMYLLEVGHVASVGGVAFGAPHCIRFSYATSDENLKIAFARIKEA
                     LAQLK"
BASE COUNT         1336 a          660 c          684 g         1265 t
ORIGIN      
        1 gaaaccattt tgggcgatac agccttgtgc attcatcctg atgatgccaa aaatgctcat
       61 ctaaaaggga aaagagttat tgtcccaatg gtcaatcgtt ccgttcctgt tattgaagat
      121 gagtatgttg acatggaatt tggaacaggt tgtttaaaag ttacacctgc tcacgacgta
      181 aacgattaca tgcttggtga aaaacatcat ttgcctgcta ttgacatttt taatgatgat
      241 ggaaccctga atgaaaatgg tacaccttac acaggaatgg atcgttttga agtaagaaaa
      301 cagatagaaa aagatttgca atcagcaggt ttacttgaaa aagtagaacc gtatgtgaat
      361 aaagtaggtt tttcagaacg aacggatgtt cctattgagc caaaactttc cacccaatgg
      421 ttcttgaaaa tgaaagaaat ggcaaaacca gcactggagg cagttattaa tgatgaaata
      481 aaattatatc caccaaagtt taaaaatact tatcggcact ggatggaaaa tgtaaaagat
      541 tggtgcattt cacgacaact ctggtgggga catagaatcc cagcatatta tttgcctgac
      601 ggtagttttg tagtagctga gaccaaagaa gaagcttacc aaaaagctct caaaaaagtg
      661 gattatcctt tgacaattga tgatttaaaa caagatgaag attgtctgga cacatggttt
      721 tcatcttggt tatggcctat ttccgttttt gatggtattc ttcatcctga taatccggat
      781 atccaatatt attatcccac cgacgattta gtaacagctc ctgaaatttt atttttctgg
      841 gtagcaagaa tgattatggc tggatatgaa tatcgtggag aaaaaccatt caagaatgtt
      901 tatctaaccg gcattgttcg ggacaaactc ggcaggaaaa tgtccaaatc actgggcaat
      961 tcacctgatc cactggattt gatccaaaaa tatggagctg atgcagtacg aatgggaatg
     1021 ttgctttgtt cgccagcagg aaatgatttg ctttttgatg aaagtctttg tgaacaaggc
     1081 agaaacttta acaacaaaat ctggaatgct tttcgcctga tcaaaggctg gacaatagta
     1141 gaaattgaac aacctatggc atctgcaaaa gctgttaaat ggtttgaaag tcaactcaat
     1201 aaatcgctcg cagaaataga cgatttgttt gcaaaatatc gtttgtctga agctttgatg
     1261 gtggcttata aacttttttg ggatgaattt gcttcgtggt ttctggaaat tatcaaacca
     1321 gcttatcaag aacctattga taaaatttct tacgaagcgg ctattcattt ttttgaaaca
     1381 ttgttaaaaa tactgcatcc ttttattcct tttattacag aagaactatg gcaaaattta
     1441 acagaaagaa aagagggtga aagcattatg attcaattat ttccacaggc tggaaaaata
     1501 gatgaaacat tgcttgaaaa ttttgaatcc acgaaacaaa ttattgctaa tatccgggct
     1561 attcgtttac aaaaaaatat tcctaataaa aagcctttaa aactgcaaat agtgggcaaa
     1621 tcccatccat attttgattc aataattgaa aaattgggta atctggatgc cattgaaaca
     1681 gtgagtgaga aatctgcaga agctgcttca tttctggttg ggacaacaga atttgctatt
     1741 ccactggctg gtttcatcaa tgtagaagaa gaaattaaaa aaattcaaaa agaaattgat
     1801 tatctggaag gatttcttgc ttcggtaacg aaaaaactga acaatcaaaa ttttctgagt
     1861 cacgcaaaac ctgaaattgt agaaagtgaa aagaaaaaac aatccgatac agaaaaaaaa
     1921 ctctcttcat taagagaaac gcttgctggt ttgatgaatt cataattttt tatattctcc
     1981 tgataataat ttgatataca attttttaaa agagattaaa ttttgggaga taatatttct
     2041 ttcaattgag aaagtgcttt gttttcttcg aaagtcattt cttctttttg ttcagaagtt
     2101 ttatcatcct gaccacataa atggagaatg ccgtgaatca ttacacgata taattcttgc
     2161 ataaaatctg tttgaaattt ttccgcatta ctttttacgg tatcaataca aataaaaata
     2221 tcgcctgaaa tcaaatttct ctcactgtca tcaaaggtga taatgtctgt ataataatca
     2281 tgctgaagat atttctgatt aatttccagt attcgatttt cattacaaaa aatgtaagca
     2341 atatctccga tttttttatc ataatctgca gctactgctt ttatccagcg attaattttt
     2401 tgttttttta tttgtggcac ttttactcct tctgaaatgt attgaatcat aataaaaatt
     2461 tagatgacgt ttaaattatt ttaaaaaagc atttaaaaac aaaacagtga aactaaaata
     2521 agaaaatagt ttcactgcat caacttttat gaaaaagatt tttacttgtt tttataaaaa
     2581 actgtttttc ttatatttat aacatttagt tatttcaact gtgccaacgc ttccttgata
     2641 cgagcgaatg caattttgag attttcgtct gacgttgcat aagaaaaacg aatacaatga
     2701 ggagcaccaa aagctacacc gccaacagaa gccacatgtc ccacttccag caaatacatt
     2761 gctaactcag cagaatcatt tatggttttg ccttgatatg attttccaat ataagcactg
     2821 caatccggaa aaacataaaa agcaccttga ggatcgttca ctttcaaacc aggaatttga
     2881 cgaatcaact caacaactaa attttttcga cgttcaaaag cttcacacat ttcacgtacg
     2941 caagtctgat cgccaatata agcagcttca gcagcctttt gagctactgc acaagctcct
     3001 gaagtatatt gtccttgcaa tttgttgcaa gcatcagcaa tccattttgg tccagcaatc
     3061 catccaattc gccagccggt catagcataa cttttggaaa caccattaat gatcacaacc
     3121 cggtcacgaa tggtttcaaa ttgtgcaatg ctttcatgtt taccagtgta attgatgtgt
     3181 tcgtaaattt catccgaaag aattataatt tgtggatatt tttccagtac ttcagcaaat
     3241 tgttttaatt cttcctttga ataaatgcta cccgtcggat tggaaggaga acaaatgatg
     3301 aaagctttgg ttttcggcgt aatggcagtt tcaagttgct gagcagtaat tttaaaatcc
     3361 tgttctatgt cggcaaaaac gccaacagat ttcccttcgg ctagtttcac catttcagga
     3421 taacttaccc aataaggtgc tggtataatt acctcgtcgc caggattaat gattgaaagc
     3481 aaaacattgc aaatagcctg ttttgcacca gtggagcaaa ttatttgatc aggtgaatat
     3541 tccagatgat tttccgattt taatttcgca caaatggctt tccgcaattc aagaaatcca
     3601 ggaacaggag ggtaaaacga aaagttgtta tcaattgctt gttttgcagc taatttaata
     3661 tgatgaggcg taaaaaaatc aggctcccca acgctgagat tgattacatc aatgcctttt
     3721 gcttttaatt cattgctttt ttgtgacatg gcaagagttt cagaaggcga aagagctgct
     3781 aaacgttttg atagattaat cataatattt cttctttttt tgttattatt attgcacaaa
     3841 attaatcaaa aaatagaaat ttcgactatt ttttaatcaa aaagtttttt agaatattta
     3901 aatgacacaa aaataaatat ttttgataaa acaataaaat cattc
//
LOCUS       DAOSAS010000146         3943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_14486_length_3943_cov_9.467850, whole genome shotgun sequence.
ACCESSION   DAOSAS010000146 DAOSAS010000000
VERSION     DAOSAS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14486_length_3943_cov_9.467850"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..2558)
                     /locus_tag="PLR72_06585"
     CDS             complement(114..2558)
                     /locus_tag="PLR72_06585"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOK36901.1"
                     /translation="MKKNIFLLTLGLIGLMSSLINCVSAQQLNTLYFMENSPVRTYYN
                     PAFQPLSKFYINLPVLGSTFIEAGNNSLTLKDVVYNKNGETILFLNPAGDKNKFYKSL
                     HNETWLNTDLQLNLLGFGFRFENSYFSFDITEKVDGGIFFPKDMFKLLLYGTPELENN
                     IFNFKNLGGSVSAYTEVALGYSKILNDQWSVGGKVKFLAGNANASLSNDHLSLNANID
                     QWLLNGKGSFKISSPAQVTIGEKLDSIEFTKPGSTSDWFKPSGMGAGLDIGFTYKPIQ
                     QLTVSAALTDLGFIKWKSNIKKVGYDMNYTFDGLVKINNLDSVDMDAFVDTLSNAFKN
                     SYTTQQVISSYTTYTSAKLNIGAEYAFLNNKMSVGLLSRTIMSSNVDEEITTSFNLRP
                     ANWFNLALSYSFMKGKMSNIGAALGLRTGFIYWNLAADYISLNNAQLKINDDYSIPIP
                     YNSKGINLGFSVNFVFGNATDKDKDGVKDSKDLCPDTPKGAKGFVDKNGCLLDTDNDS
                     VPDYLDKCPGTPKEARGFVDKNGCLLDTDNDGIPDYLDKCPNTPSGVVVDSVGCPLDA
                     DKDGVPDYLDKCPDTPQEAYGFVDANGCLIDSDLDGVPDYIDKCPNTPAEAIGKVDEN
                     GCPLDTDNDGVPDYLDQCPNTPAEAAGTIDEKGCPRDTDGDGIPDYKDNCPKTPGDST
                     NQGCPQVKKEIQTLFQKALHGIQFETGKYKIRPFSYPILNQIAQMLIENPIYQIEIQG
                     HTDNVGNPKKNIELSEKRAMAVKEYLVSKGVDENRITTKGYGDTVPVASNETAMGRAL
                     NRRVEFLITFKEIKYE"
     gene            complement(2568..>3943)
                     /locus_tag="PLR72_06590"
     CDS             complement(2568..>3943)
                     /locus_tag="PLR72_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36902.1"
                     /translation="PLPPSQTIPANQSANFENNLGIDLGLNAHFEEERIDSAKIQSAI
                     LSIKISPENLAISPNNIELTIEFATSHMQTFNGEPVTVSYNPSFFNQVEEKTIRNFVL
                     NTSQNSIIPLILKLKITAGDSPINISPSSKITIEVKFTHFDFEVAYGFFAPSVDAMVI
                     EQQHLNLPSFTNDGDFQFANPQFFFNVKSNIGTYLYFKVDYIKAFKQENPSQEVYARF
                     SNDQDSLSTKEALGNKPSNPGEYIAKQLRVLDKDYGATDRLFSINPQPDVLEYKFSTI
                     LDQKKINQDHSANFITPDAQIKVKYKIKIPFNLKSQSYYTSQDTVKDVDIDSTLKDVK
                     VDSAQIVLKVENGLPVKVTLNMVLLDGNNKPINTTFKTSYQIDAPQIVDGYVVPDGIA
                     MQKIIITLNNNQFNDFKQTKNIVFLITINSKDINSNIHFQKSNSFHAKMSLFVKGSYT
                     YTSDNSK"
BASE COUNT         1257 a          661 c          627 g         1398 t
ORIGIN      
        1 tgagtttgga atacaaattt acaacccagg gacgagactt tcttcttgtc ccttcttttt
       61 tatcttatct tttgtcggtc agtagtgatt aataataata ataataataa tttttattcg
      121 tattttattt ccttgaaagt tataaggaat tcaactcttc tgtttaatgc tctacccata
      181 gcagtttcat tagaagctac cggaactgta tcgccatagc cttttgtcgt aattctattt
      241 tcatcaactc cttttgaaac aagatactct ttaactgcca tagctcgttt ttcagagagt
      301 tctatatttt tcttaggatt acccacatta tcggtatgtc cttgaatttc tatctgataa
      361 ataggatttt caatgagcat ttgagcgatt tgattcaaaa taggatacga aaatggtctt
      421 attttatatt ttcctgtttc aaattgaatt ccatgtaacg ctttttggaa aagcgtttgt
      481 atttcttttt ttacttgtgg acaaccctga ttggtactat cgcctggagt tttaggacaa
      541 ttatctttat aatcagggat accatctcca tcagtatcac gcggacaacc tttttcatca
      601 attgttccag ctgcttcagc aggcgtattg ggacactgat ccaaataatc aggtactcca
      661 tcattatctg tatctaacgg acaaccattt tcatcaactt ttccaatagc ttctgcaggt
      721 gtatttgggc atttatcaat ataatcaggg acaccatcaa gatcactatc aattaaacat
      781 ccatttgcat ctacaaagcc ataagcttct tgaggggtat cagggcattt gtctaaataa
      841 tcagggactc catctttatc agcatccaat ggacatccaa cagaatctac tactacacct
      901 gacggggtat taggacattt gtctaaataa tcaggaatac catcattatc tgtgtcgagt
      961 aaacatccat ttttatctac aaatcctctt gcttcttttg gtgtaccagg acatttatct
     1021 aaataatcag gaacgctatc attatccgta tcgagtaaac atccattttt gtctacaaat
     1081 cctttggcac cttttggtgt atcgggacat aggtctttgc tgtctttcac accatctttg
     1141 tctttgtcag tagcatttcc aaaaacgaaa ttcacactaa atcctaaatt tattccttta
     1201 gaattataag ggatagggat cgaataatca tcatttatct ttaactgtgc attattcaat
     1261 gaaatataat cagctgctaa attccagtaa ataaaacctg ttcttaatcc caatgccgca
     1321 ccaatattgc tcattttccc tttcataaat gaataggaca atgccagatt aaaccagtta
     1381 gcagggcgaa gattgaaaga agtagtaatt tcttcatcaa cattgctact cattatggta
     1441 cgtgaaagta aacctacact cattttgttg ttcaaaaaag catattcagc tccgatattt
     1501 aatttagctg acgtataagt agtataagaa gaaatcacct gttgagttgt ataagaattt
     1561 ttgaaggcat tggacaatgt atccacaaaa gcatccatat ctactgaatc cagattgttt
     1621 attttaacca atccatcaaa tgtataattc atgtcatatc ctactttctt gatgttcgat
     1681 ttccacttga tgaatcctaa atcagttaaa gcagctgaaa cggttaattg ttgaataggc
     1741 ttgtaagtaa agccaatgtc taatccagca cccattcctg aaggtttgaa ccaatcactg
     1801 gttgagccag gtttagtaaa ttcaatggaa tccagttttt caccaatggt tacttgagct
     1861 ggagaagaaa ttttgaacga tcctttacca tttaatagcc attgatcaat attggcattc
     1921 aaacttaaat gatcattaga gagagaagca tttgcatttc ctgccagaaa ttttactttt
     1981 cctcctactg accattgatc gtttaaaatt tttgaatagc ccagtgcaac ttctgtataa
     2041 gccgagacac tccctcctaa attcttaaaa ttaaaaatat tattttccaa ttctggagtt
     2101 ccataaagta gtaatttaaa catatctttc gggaagaaga ttccgccatc tactttttca
     2161 gtgatatcaa atgaaaagta agaattctca aaacggaagc caaaacctaa taaattaagt
     2221 tgcaaatcag tattcaacca tgtttcgtta tgtaaagatt tataaaattt attcttatcc
     2281 cctgcagggt tgagaaacaa aattgtttcg ccatttttgt tgtacacaac atcttttaac
     2341 gtaagagaat tattacctgc ttcgataaaa gtagagccta aaaccggaag atttatataa
     2401 aacttgctta aaggttgaaa ggcaggattg taataagttc gaacaggtga attttccata
     2461 aaatataggg tgttaagctg ttgtgcagat acacagttaa taagtgaact catcaagcct
     2521 atcaatccta aggtaagaag aaaaatgttt tttttcatgg ttttcgatta tttagaatta
     2581 tcacttgtat atgtgtaact tccttttaca aaaagactca tttttgcatg aaaactattt
     2641 gatttctgaa aatgtatatt ggaattaatg tctttgctat ttatggtgat taagaaaaca
     2701 atatttttag tttgtttgaa atcgttaaat tggttattat ttaaagtaat aataattttt
     2761 tgcatggcaa tgccatctgg tacaacataa ccatcgacta tttgaggagc atctatttga
     2821 taagaagttt taaatgtagt atttattggt ttgttgtttc catccagtaa gaccatgttt
     2881 aaagtaactt ttacaggtaa accgttttct acttttaaaa ctatttgagc actgtcaacc
     2941 ttcacatctt ttaaggtaga atcaatgtca acatctttta cagtatcttg agatgtataa
     3001 taactttgag attttaaatt aaaaggaatt tttattttat attttacttt gatttgagca
     3061 tcaggggtta taaagtttgc agaatgatcc tgattaattt ttttttgatc taaaatcgtt
     3121 gaaaacttat attccaatac atctggttgg gggttgattg aaaagagtcg atctgtagca
     3181 ccatagtctt tgtccaaaac gcgtaattgc tttgctatat attctcctgg atttgatggt
     3241 ttattgccta acgcttcttt agttgataaa ctatcttgat cattactaaa ccttgcataa
     3301 acttcttgag agggattttc ttgtttgaag gcttttatat agtcaacctt gaaatacaga
     3361 taggttccaa tattactttt aacattaaag aagaattggg gatttgcaaa ttgaaaatca
     3421 ccatcatttg taaatgaagg aagattcaaa tgttgctgct caattaccat tgcatcaaca
     3481 gagggtgcaa aaaagccata agctacctca aaatcaaaat gagtgaattt cacttcaatg
     3541 gtaattttag atgagggaga aatgttaata ggtgaatctc cagccgtaat tttcaatttt
     3601 aagatcaaag gtataataga attttgcgaa gtatttaata caaaatttcg aatggttttt
     3661 tcctctactt gattaaaaaa agacggatta taactcactg taacaggttc accattaaat
     3721 gtttgcatat gacttgtagc aaattctatg gttaattcaa tattattagg cgagatggca
     3781 agattttcgg gtgatatttt tatacttaat atagcagatt gaattttcgc actatcaatt
     3841 ctttcttctt caaaatgagc atttaaaccc aaatcaatac cgagattgtt ttcaaaatta
     3901 gctgattgat ttgctggaat agtttgtgaa ggtggtaaag gaa
//
LOCUS       DAOSAS010000147         3904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_14684_length_3904_cov_18.928553, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000147 DAOSAS010000000
VERSION     DAOSAS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14684_length_3904_cov_18.928553"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1700
                     /gene="ispG"
                     /locus_tag="PLR72_06595"
     CDS             <1..1700
                     /gene="ispG"
                     /locus_tag="PLR72_06595"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOK36903.1"
                     /translation="PIRLQSMTNTDTNDIDASVSQCIRLIKVGAELVRLTTQGLREVE
                     SLKKIHQQLNEKKFYVPLIADVHFNHLVAEAAAGCVEKVRINPGNYVRSIKINHSADY
                     TEEEFNLEQQKLKEQFIRLINICKEHRTAIRIGVNHGSLSERMMNRYGDTPEGLVASC
                     MEFLQICKEENFSDVVVSIKASNTVLMIHAVRKLVKEMEQKGFPYPIHLGVTEAGEGE
                     DGRIKSAVGIGTLLADGIGDTIRVSLSEEPEAEIPVARQLVSYIAEREQHEKIFAQIN
                     PSWSPFEYFCRKTQAVANIGGNHLPVVFATTQKNFTISPDYYIEGRKIYNNAGKIFSV
                     YLPDEIPLLKKDVSEVKFLKLSYPELNSDVLSFLKERKDVVILLFTKHKNGVAEQRAF
                     FHTLMNKDITTPVIIARNYQENNLESLQIKASVDIGMLFVDGFGDGILLQNEGTISSK
                     DINSLSFGILQATRVRISKTEYISCPGCGRTLFDLQKVVAEIKAKTSHLKGLKIAVMG
                     CIVNGPGEMADADYGYVGARKGKVSLYKGKQCLLKNIAEDEAINKLIELIKVNGDWL"
     gene            1797..2267
                     /locus_tag="PLR72_06600"
     CDS             1797..2267
                     /locus_tag="PLR72_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36904.1"
                     /translation="MKKTIFIAILLVSFMTSFAYGQVNGKAIGVRASYGAELSYQHPF
                     SQANRLEADLGFYYDHGFNLAGIYQWVFDLSELANGFNWYVGLGPQIGSWDYHHNGKD
                     YDGLALGIAGQIGIEYNFDIPLQLSLDYRPGWYILPNSYGGAWEGVALGIRYKF"
     gene            complement(2355..>3904)
                     /locus_tag="PLR72_06605"
     CDS             complement(2355..>3904)
                     /locus_tag="PLR72_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK36905.1"
                     /translation="FPVKYFNKNAVVTVTPVLRFQGQEVKSTPATFQGEKVIGNNKVI
                     SYKAGGSYTINASFNYVPEMAKSELYLDFDVTVKNKNYQIPSVKVADGVIATSELAVV
                     NANEIQGALIPDKFQRIIQESQEADIKFLIERWDLRPTELKKDEVISLVNKITQVKND
                     TAKKVSDFEIAGYASPDGPIDLNTNLAEKRQKVSVDYINNQMKKLKTSVKIDSKFTPE
                     DWEGFQKLMENSNIQDKEVILRVLSMYSDPEERERQIKNLSAAYQKIAEEILPQLRRS
                     RLKLTVDIIGKSDDQISQLAANNPSQLSVEELLYAATLKNNLNDKAIIYQEVINLYPN
                     DIRGYNNLGLIYYQQDKIDEAEKLFTQALQKDPNSPDVNFNLGLIALKKGDVTKAEEL
                     FGKAANTSGELSKALGTLYLKKGDYAKAMNSFKSNDISNNVALLYILNNDYNQARKVL
                     AAIPQPDATTAYLSAIVGARTNDRDAVYSNLRTAISKDKSLAQKALNDIEFAKFATDS
                     TFLSIVK"
BASE COUNT         1268 a          606 c          722 g         1308 t
ORIGIN      
        1 cacctattcg tttgcaatcg atgactaata ctgataccaa cgatatcgat gcttctgtat
       61 cgcaatgtat tcgtttgatc aaggttggtg cagaactggt gcgacttact acacagggat
      121 tacgcgaagt agaaagttta aaaaaaattc atcagcaatt aaatgaaaaa aaattttatg
      181 tcccgttaat agcagatgtc cattttaacc atcttgtagc tgaagctgct gctggatgtg
      241 tagaaaaagt acgaattaat ccaggtaatt atgtgagaag tattaaaata aatcattcag
      301 cggactatac ggaagaagag tttaatttag aacagcaaaa attaaaagaa caattcatac
      361 ggcttatcaa tatttgtaaa gaacatcgca ctgctattcg tattggagtg aatcatggtt
      421 ccttgagtga aagaatgatg aatcgttatg gcgatactcc agaaggatta gttgcctcct
      481 gcatggaatt tttacaaatt tgcaaggaag aaaatttttc tgatgtagtt gtttctataa
      541 aagcttccaa tactgtttta atgatacacg ctgtgcgaaa gcttgtaaaa gaaatggaac
      601 agaaaggttt tccttatccc attcatttag gagtaacaga agcaggggaa ggtgaagatg
      661 gtcgcatcaa atcggcagtt ggaatcggaa ctttactagc agatggaatt ggtgatacca
      721 ttcgtgtttc tttgagtgaa gaaccagaag ccgaaattcc tgttgcacgg caacttgtat
      781 cttatattgc tgaacgcgaa caacatgaaa aaatttttgc acagattaat ccttcatggt
      841 cacctttcga atatttttgt aggaaaaccc aagccgttgc caatataggt ggaaatcatt
      901 tgccagtagt atttgctact acacaaaaaa atttcaccat ttcaccagat tattacatag
      961 aagggagaaa aatctataat aatgctggaa aaattttctc tgtttatttg ccagatgaaa
     1021 ttcctttact aaaaaaagac gtatcagaag taaaattttt aaaattgtct tatccagagt
     1081 tgaattctga tgttttatcg tttctgaaag aaagaaaaga tgtagttatt ttgcttttta
     1141 caaaacacaa aaatggagtt gctgaacaaa gagctttttt ccatactttg atgaataaag
     1201 atataactac tcccgtcata atagctcgaa attatcaaga aaacaattta gaaagtttac
     1261 aaataaaggc gtcggttgat attggtatgt tatttgtaga cggcttcggc gacggaattt
     1321 tgttgcaaaa tgaaggtacc atatcctcaa aagatattaa ttcgctgtca tttggaattt
     1381 tacaggctac gcgagtaaga atttcaaaaa cagaatatat ttcgtgcccg ggctgtggac
     1441 gcaccttatt tgacttgcaa aaagtagttg cagaaataaa agctaaaact tctcatttaa
     1501 aaggtttaaa aattgctgta atgggttgta ttgtaaatgg gcccggcgaa atggcggatg
     1561 cagattatgg ttatgttggt gcaaggaagg ggaaagtaag tttgtataaa ggcaagcaat
     1621 gtttgttaaa aaatattgcc gaggatgaag ctataaataa attaattgaa ctcataaaag
     1681 tgaatggtga ttggctataa aaagactgaa aaaaataaaa tattatcttt tctaattttt
     1741 tcttttaaaa taatgtgatt ctcttaaaaa tggtttaaca attaaaaaat gaaggtatga
     1801 aaaaaacaat ttttattgcg attctattgg taagctttat gacgagcttt gcttatggac
     1861 aggtaaacgg taaagctatt ggagttcggg ctagttatgg tgctgagtta tcttatcaac
     1921 atccttttag tcaagctaat cgccttgagg cagacttagg cttttattac gatcacggtt
     1981 tcaatctcgc tgggatttat caatgggttt ttgatttatc agaattagca aatggtttta
     2041 attggtatgt aggtttagga cctcaaattg gatcatggga ttatcatcat aatggtaaag
     2101 attatgatgg tctggcttta ggaatagcag gacagattgg gattgaatat aattttgata
     2161 ttcctttgca actttcattg gattatcgtc caggttggta tattttacca aatagttacg
     2221 gaggagcctg ggaaggagta gctttaggaa tacgttataa attttagagg taagaaaaac
     2281 ttttttgaaa tggttgaatg aaaaaagagg cagcttattt tagctgcctc ttttttagta
     2341 ttaataaagt tatcctactt tacaattgac aggaaagtag aatcagtagc aaattttgcg
     2401 aattcaatat cattcaatgc cttttgagct aatgatttat ctttgctaat agctgttctt
     2461 aaatttgaat aaacggcatc acgatcattt gttctggctc caacaatagc tgaaagataa
     2521 gctgttgtag catcaggttg tggaatagca gctaaaactt ttcgagcctg gttataatca
     2581 ttattcagga tatacagcaa agctacattg tttgatatgt cattcgattt aaacgaattc
     2641 attgcttttg cataatcacc ttttttcaaa tacaatgttc ctaaagcttt acttaattct
     2701 ccacttgtat tggctgcttt tccaaataat tcttcggctt tggtcacatc gcctttcttt
     2761 aatgcaatca atcctagatt aaagttaaca tcaggtgaat ttggatcctt ctgcagagct
     2821 tgagtaaaca atttttctgc ttcatctatc ttgtcttgtt gatagtaaat aagacctaaa
     2881 ttattatacc cacgaatatc atttggataa aggtttatta cttcctgata aataattgct
     2941 ttatcattca gattattttt taaggtcgca gcataaagca actcttcaac agaaagttgc
     3001 gatggattgt tagcagctaa ttgagagatt tgatcatcag atttaccaat aatatccacc
     3061 gtaagtttca gtcgagaacg acgtaattga ggaagaatct cttctgcaat tttttgataa
     3121 gctgcagata gatttttaat ctgacgttca cgttcttctg gatcagaata catcgacaaa
     3181 actcttaaaa taacttcttt atcctgaata ttggaatttt ccattaactt ctggaatcct
     3241 tcccagtctt cgggtgtaaa ctttgaatca atttttacag aagttttaag cttcttcatt
     3301 tgattgttga tataatcaac agaaaccttt tgacgttttt cagcgagatt ggtatttaaa
     3361 tcaattggac catcaggtga agcatatccc gctatttcaa aatcagaaac ttttttagca
     3421 gtatcatttt taacctgtgt tattttatta accaacgaaa tgacttcatc cttctttaat
     3481 tcagtaggtc gaagatccca tctttcgatt aaaaatttta tgtctgcctc ctggctttct
     3541 tgaataatac gttgaaattt atcaggtatc aaagcacctt gaatttcatt tgcattaaca
     3601 acagccagct cagaggttgc tataacacca tctgcaactt tgacgcttgg aatctggtaa
     3661 ttcttgtttt taacagtgac atcaaagtca agatataatt ccgattttgc catttctggt
     3721 acataattga aagaagcatt aatggtataa gaaccaccag ccttgtaaga aatgacttta
     3781 ttatttccta ttactttttc gccctggaaa gttgcagggg tacttttcac ttcttgaccc
     3841 tgaaaacgta aaacaggagt taccgttact acggcattct tgttaaaata ttttacaggg
     3901 aaag
//
LOCUS       DAOSAS010000148         3868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_14876_length_3868_cov_13.088120, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000148 DAOSAS010000000
VERSION     DAOSAS010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14876_length_3868_cov_13.088120"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /gene="holA"
                     /locus_tag="PLR72_06610"
     CDS             complement(<1..1009)
                     /gene="holA"
                     /locus_tag="PLR72_06610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOK36906.1"
                     /translation="MAKQNFTYEDVLNDLRQKIYFPVYFLMGEEPYYIDLVSDYIQNN
                     ILTETEKDFDQTIFYGKDVNIPTIINVAKRFPMVAKHQVLIVKEAQLIDTDEWEKLSY
                     YLKNPLESTILVFCYKYGTPDKRKKWFQDISRAGVVFESKKLYENQVGPWIRSYLKSQ
                     NISITEKAINMLVEFLGNDLSKIMNALEKLLINRNENFKEITPELVEKYIGISKDYNV
                     FELQSAIIDKNILKANRIIRYFGDNKKANPLPFVLIQLFNFFNNLLLYYYLKDKSEKT
                     VALELKINPYFVKDYVKAASNFTPQKTIQIISDIRQTDARSKGVDQTTIEEVELLKEL
                     IF"
     gene            complement(1026..1799)
                     /locus_tag="PLR72_06615"
     CDS             complement(1026..1799)
                     /locus_tag="PLR72_06615"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOK36907.1"
                     /translation="MKTKKEIVENWLPRYTKRPLEDFTNHILLVNFNIYLELFANYYQ
                     VPIIGRNGNMPNASANGITMINFGLGSPNAALIMDILSAVQPKAVLFLGKCGGLRDKN
                     KEGDYILPIAAIRGEGTSNDYFPPEIPALPAFSLQRAVSSNIRDFGKDYWTGTVYTTN
                     RRVWEHDEKFKEYLRSTKAMAIDMETATLFSSGFYNGIPTGALLLVSDMPMHEEGIKT
                     IESDNRVNANYVEEHLKIGIASLVSLITNSRTVKHLRFE"
     gene            1887..2354
                     /locus_tag="PLR72_06620"
     CDS             1887..2354
                     /locus_tag="PLR72_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOK36908.1"
                     /translation="MLQLNLPAYNFRLKKERDTYWIFDNIRKKFVKLTPEEWVRQNFI
                     RFFTEEKHFPASRLSVEEEIVWNGMKKRCDAIFYNEYAEPALILEFKAPQITISQLVF
                     DQVAVYNVKLNVNFFIISNGIEHFFCKVNVKNSQFEISREIPDYKIFIELNRQ"
     gene            complement(2364..3278)
                     /locus_tag="PLR72_06625"
     CDS             complement(2364..3278)
                     /locus_tag="PLR72_06625"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOK36909.1"
                     /translation="MVKLNVDIGKLQLKNPVMTASGTFGYGIEYADFLDLSRIGSIIV
                     KSITLRNREGNPYPRMKETASGMLNAVGLQNKGVKYFVENIYPLLKNIDTCIIANIAG
                     STIEEYVQIAEILNELDKISALELNISCPNVKEGGMLFGNSSIVANQVVTAVRRAYSK
                     ELIVKLSPNVTSISEIAIAVESAGADAISLINTLVGMAIDIETRKPCLSTITGGLSGP
                     AIKPVAVRMVWEAAHAVKIPIIGIGGIMNAADAIEFLLAGATAIEVGTANFMDPTVTI
                     KIIEGIEQYLIQHGFDNVYEIIGAMENK"
     gene            complement(3272..>3868)
                     /locus_tag="PLR72_06630"
     CDS             complement(3272..>3868)
                     /locus_tag="PLR72_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOK36910.1"
                     /translation="IHYVDKKKNELWLLIQVVGEGSRKLYHINKNEILNLILPLGNSF
                     SLPKNHKDKILVIGGGTGIAPLLFLGDYLHKNGFCPNFLFGARNKNYLIPLKKFETYG
                     NIFITTEDGSTGEKGLVTCHSILKNHFDMIYCCGPTSMMKAVASYAEKNNIPCEVSLE
                     NTMACGFGVCLCCVTDTTEGRVCVCTEGPVFNITKLKW"
BASE COUNT         1278 a          688 c          534 g         1368 t
ORIGIN      
        1 tgaaaatcaa ttcttttagt aattctactt cttctattgt agtttgatca acgcctttgc
       61 tcctggcatc tgtttgcctt atatcggaaa taatctgaat ggttttttgg ggtgtaaaat
      121 tagaagctgc ttttacataa tcttttacaa aataaggatt gattttaagt tctaaagcaa
      181 ctgttttttc acttttatct tttaaataat aatacagcaa caaattatta aaaaaattga
      241 acaattgaat taagacaaat ggcaatggat ttgctttttt gttatctcca aaataacgta
      301 taatcctatt tgcctttaaa atatttttat caatgatggc tgattgaagc tcaaaaacat
      361 tgtaatcttt actaatgcct atatattttt caacgagttc aggcgtaatc tctttaaaat
      421 tttcattccg attaatcaaa agcttttcaa gagcattcat aattttactt aaatcattcc
      481 ctaaaaattc aaccagcata tttattgctt tttcggtaat agagatattt tgagatttta
      541 aataagaacg tatccacgga cccacttgat tttcataaag ttttttagat tcaaaaacta
      601 ccccagcacg tgatatatct tgaaaccatt tttttcgttt gtcaggggta ccatatttat
      661 aacaaaaaac taaaatggtt gattctaaag gatttttcaa ataataactc aatttttccc
      721 attcatcggt atcaataagt tgagcttctt ttacaatcaa cacttgatgt tttgccacca
      781 ttggaaatcg cttggcaaca ttaataatag taggtatatt tacatctttc ccataaaaaa
      841 tggtttgatc aaaatctttt tctgtttcag tcaaaatatt gttttgaata taatccgata
      901 ccaaatcaat atagtaaggc tcttctccca ttaaaaaata aacaggaaaa taaattttct
      961 gccgcaaatc atttaaaaca tcttcgtaag taaaattttg cttagccatt tttaacagtt
     1021 attttttatt caaatcttaa atgctttaca gttcgactat tggtaatcaa ggaaactaat
     1081 gaagcaatgc caattttcaa atgttcttct acataattgg cattcactct attatcgctt
     1141 tcaatggttt ttatcccttc ttcatgcata ggcatatcgg aaactaacaa taaagctcct
     1201 gttggaattc cattataaaa tcctgaggaa aacaacgtcg ctgtttccat atctattgcc
     1261 atagctttgg tagaacgaag atattcttta aatttctcat catgttccca tactcttcta
     1321 ttagttgtgt aaacggttcc tgtccagtaa tctttcccaa aatcacgaat atttgaagaa
     1381 accgcacgct gaaggctaaa agcaggcaag gctggaattt ctggcggaaa ataatcgttc
     1441 gaagttcctt cacctcgaat agcagcaatg ggtaaaatat aatcgccttc tttattttta
     1501 tctcttaaac caccacattt acctaaaaac aaaacagctt ttggttgtac tgctgacaaa
     1561 atatccataa taagagcagc atttggactg cccaaaccaa aattaatcat cgttatacca
     1621 ttcgctgagg cattgggcat atttccattt cttccaataa taggaacttg ataataattg
     1681 gcaaacaatt ccagataaat attaaaattt accagcaaaa tatgattggt aaaatcttcc
     1741 aatggtcgtt ttgtatatcg gggtaaccag ttttctacta tttctttttt cgttttcatc
     1801 ttgtgtcgtt ttttgataca tttttgtata ttcgtatttt atttctggta ggaaatagat
     1861 cgagatatag agcaaaatta caaaaaatgt tacaattaaa tttgccagct tacaattttc
     1921 gtttaaaaaa agaacgagat acttactgga tatttgataa tatccgaaaa aaatttgtaa
     1981 aactcactcc tgaagaatgg gtaagacaaa attttattcg tttttttacc gaagaaaaac
     2041 atttccctgc cagtcgtttg tccgttgaag aagaaattgt ttggaatgga atgaaaaaac
     2101 gttgcgatgc tattttttat aatgaatatg ctgaacctgc cttgatatta gaatttaaag
     2161 ctccacaaat aactatctct caattagttt ttgatcaagt ggctgtttat aatgtaaaat
     2221 taaatgtaaa tttttttatc ataagcaatg gaatagaaca ttttttctgt aaagttaatg
     2281 taaaaaattc gcagtttgag atttcaagag aaatacctga ttataaaata tttatcgaat
     2341 taaatagaca ataaaaataa tttttattta ttttccattg ccccaataat ttcataaaca
     2401 ttgtcaaaac catgttgaat caaatattgc tcaattccct cgattatttt aatggtcact
     2461 gttggatcca taaaattagc cgttcccact tctattgcag tagctccagc cagtaaaaat
     2521 tcaatagcat cagcagcatt cataattcct ccaataccaa taattgggat tttaacggca
     2581 tgagctgctt cccaaaccat tcgaacagct acgggtttga ttgcaggacc agacaagcct
     2641 cccgtaatgg tggaaaggca aggttttctg gtttctatat caattgccat tcctaccaat
     2701 gtgttaatta aggatatggc atcagcacct gcactttcta cagcaattgc tatctctgaa
     2761 atactcgtta catttggcga aagctttaca attagttctt tactatatgc ccttcgcact
     2821 gctgtaacta cttgattagc aactattgac gagtttccaa aaagcatccc tccttccttc
     2881 acatttggac aggaaatgtt caattctaag gcagaaattt tatcaagctc attcaatatt
     2941 tcagcaattt gaacgtattc ttcaatggtt gaacctgcaa tgttggcaat aatacacgta
     3001 tctatatttt ttaaaagagg ataaatgttt tcgacaaaat attttactcc cttattttgc
     3061 agtcctacag catttagcat gcctgacgca gtttctttca tgcgaggata tggattccct
     3121 tctctattgc gaagtgtaat actttttaca ataatactac ctattcgtga taaatcaagg
     3181 aagtctgcat actcaattcc atagccaaaa gtacctgagg ctgtcatcac aggatttttc
     3241 aactgtaatt ttcctatatc gacatttagt tttaccattt taattttgta atattaaaaa
     3301 ccggaccttc tgtacaaaca caaactcttc cctctgtggt atctgttacg caacataaac
     3361 aaactccaaa tccgcaagcc atcgtatttt ctaaggatac ttcacacggg atgttatttt
     3421 tttcagcata agaagctaca gctttcatca tggatgttgg accacaacaa taaatcatat
     3481 caaaatgatt ttttaaaata gaatggcaag ttaccaaacc tttttctccc gttgagccat
     3541 cttcggtagt aataaagata tttccgtaag tttcaaattt ttttaaagga attaaatagt
     3601 ttttatttcg agctccaaat aaaaaatttg ggcaaaaacc atttttatgt aaataatcac
     3661 ccaaaaaaag taaaggagct atacctgttc cgcctccaat aactaaaatt ttatctttat
     3721 gattttttgg caagctaaaa gaattgccca aaggtagtat taaatttaaa atttcatttt
     3781 tattgatatg atacaatttt ctgcttcctt ctcctaccac ttgaattaat aaccataatt
     3841 catttttttt cttatcaaca taatgaat
//
LOCUS       DAOSAS010000149         3857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_14926_length_3857_cov_13.925302, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000149 DAOSAS010000000
VERSION     DAOSAS010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14926_length_3857_cov_13.925302"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..2593)
                     /gene="feoB"
                     /locus_tag="PLR72_06635"
     CDS             complement(104..2593)
                     /gene="feoB"
                     /locus_tag="PLR72_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOK36911.1"
                     /translation="MYEINNFTRLSDLSPGDEAIIVKVLGHGSFRRRLAEMGFVKGKK
                     VKVIECAPLQDPIEYEIIGYNISLRRIEAELIQVISVQEATSVLQNIHFNGIIDDETL
                     KVSALEKGKSIHVALVGNPNCGKTTLFNRISGAHERVGNYGGVTVEIKEAEVKKNGYT
                     IKLVDLPGTYSITEFSPEELYVRVYLTEKMPDIVLNVIDASNLERNLFLTTQLIDMDL
                     KVVIALNMYDELEKKGDKLDYKALGKMLGIPIVPTVATKGEGIEELLQKIIDVYEDKD
                     PIVRHIHINYGSVIEKAIKQIQDVIWQNPIITDKLSSRYLAIKLLEGDKAILSELEKY
                     ENYPQIKSITKQAIQNLEKEYGDTCDTVITQLKYGFIEGALKETYHLSNQVNKIKQQT
                     LDNLFLHKFWGYPIFLFFILLMFQVTFSLGKYPTEWLESGINLLSGWVEKIMSDGPLR
                     DLLANGIIGGVGNVLVFFPNILILFFFISILEDTGYMARAAFIMDKLMHKIGLHGQSF
                     IPLVIGFGCNVPAILSTRTLKNRKNRILTILIIPFMSCSARLPVYLLLISAIFSKNQA
                     LILFSLYLIGILIAVITALIMKKIAFKEEESPFVMELPPYRIPTLKTINIHMWDKSRE
                     YLRKIGNIILVASIIIWAIGYFPRQPEIAKKYEKQILAIQNNTSLNEKEEEKTIFQLQ
                     TAKASELQQKSFIGQLGKAIEPVIRPLGFDWKMGVSLLTGFAAKELIISTMGILYQVD
                     TSGQSSVSLQQKIKEEFHTTGKLKGERVFTPLVAYAFMVFILLYVPCVATIAAMKKEA
                     GWQWAIFGIVYNTAIAWLAAFLIYQIGSLLL"
     gene            complement(2606..3718)
                     /locus_tag="PLR72_06640"
     CDS             complement(2606..3718)
                     /locus_tag="PLR72_06640"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HOK36912.1"
                     /translation="MEWHRVRKTLLIGIILLHNVIKLAAQEKNETSFFTFSASYIGDG
                     FANMSGGIKTGISYLGLISLNTYFDTSKAHLWKDGGLFLNIFNTHGGQPTAHFVGDYQ
                     GVSNIEASNHTYLYELWYEQKFKKVKSIIGLQDLNVNYAASEYGSLFINSSFGIPSTI
                     AYNIPSPIFPLTALGFHLEWQPTQHFKWKAAIFDGTPEGFENFHLKWKLNKQNGFLNI
                     TEMEWNKNFQENRSGIYKIGAYYHNSRDSMQHTEENRGFYFVGDQTVNKHVGIFSQIA
                     ASPASKNSNPFYLGIGFNYKGIGKKRQEDIFGLALAYASLKNRRDEATIEMIYQFVIN
                     RHISLMPDIQYVINSSGTDIALKNSLISFLRMRLDF"
BASE COUNT         1296 a          719 c          549 g         1293 t
ORIGIN      
        1 aaccaatcgc cgttgataaa ataactatta ctgaaatgat ttgaaacatc attttcaatt
       61 aaaatttcat tttaacaaat tctcaaccta attagttcag ttattataac aataaacttc
      121 ctatttgata aattagaaaa gcagcaagcc aggctattgc agtattataa acaatcccaa
      181 aaatagccca ttgccagcct gcttcttttt tcatagctgc aatagtagct acacaaggaa
      241 cataaagcaa aataaatacc ataaaagcat aagccaccag cggagtaaaa actctttctc
      301 ctttcaattt acctgtggta tgaaattctt ctttaatttt ttgctgtaat gagactgaac
      361 tttgaccaga agtgtcaacc tgatatagaa tacccattgt gctaattatc aattctttgg
      421 cagcaaatcc tgttaacaga ctaacaccca ttttccaatc aaaacccaaa ggtctgatga
      481 caggttcaat tgcttttccc aattgtccaa taaaagattt ctgctgtaat tcagaagctt
      541 tagccgtttg aagttgaaaa attgtttttt cctcttcctt ctcatttaat gaagtattgt
      601 tttgaattgc caaaatttgt ttctcatatt ttttcgcaat ttctggttgg cgaggaaaat
      661 agccaattgc ccagattata attgaagcta ccagaataat atttcctatt tttcgcaaat
      721 attcccgact tttatcccac atgtgaatat taatagtttt taaagtagga atcctatagg
      781 gaggcaattc cattacaaag ggtgactcct cttccttgaa tgctattttc ttcattatca
      841 atgctgttat cacggcaatt aaaatcccaa tcaaataaag tgaaaataaa atgagtgctt
      901 gatttttaga aaaaatggct gaaataagca acaaataaac aggcaatcga gcactacacg
      961 acataaaagg aataatgagt atggtcaata ttcggttttt tctatttttt aacgtacgcg
     1021 ttgacagaat agcaggaaca ttgcagccaa atcctatcac caatggaata aacgattgtc
     1081 catgaagtcc aattttatgc attaacttgt ccattataaa ggcagctcgc gccatatagc
     1141 ctgtgtcttc gaggatggaa ataaagaaaa ataaaatgag aatattcgga aaaaagacaa
     1201 gcacattccc tactccacca ataataccat ttgccagcaa atcacgcaaa ggaccatcag
     1261 acattatttt ttcaacccat ccagacagta aattaattcc actttccagc cattcggtcg
     1321 gatattttcc cagactgaaa gtaacctgaa acattaacaa aatgaaaaac agaaagatag
     1381 gatatcccca gaacttatga agaaacaaat tgtctaaagt ttgttgcttt attttattga
     1441 cttgattaga aagatgataa gtttctttta aagccccttc aataaaacca tattttagtt
     1501 gggtaataac agtatcgcaa gtgtcaccat attctttttc cagattttga atagcttgct
     1561 tcgtaataga tttgatttga ggataattct catatttttc tagttctgag agaatggctt
     1621 tatcaccttc caacaatttg atagccagat aacgggaaga taatttatca gttataatag
     1681 gattttgcca gattacatcc tgaatttgtt tgatagcttt ctcaattaca gaaccataat
     1741 taatatgaat atgcctaaca atgggatctt tatcttcgta aacatctatt attttttgaa
     1801 gaagttcttc aataccttca cctttggtgg caacagttgg gacaatgggt attcccagca
     1861 tttttcctaa agctttgtaa tcaagtttat cccctttttt ctctaattca tcatacatat
     1921 tcaaggcaat cacaaccttc aaatccatgt caatgagctg agtagtcaaa aataaattac
     1981 gttccaaatt ggaagcatct atcacattga gcacaatatc aggcattttt tcggttaaat
     2041 aaacgcgaac ataaagttct tctggagaaa attctgtgat agaataagta ccaggcaaat
     2101 caaccagctt aatagtatag ccattttttt taacttcggc ttcctttatt tctacagtga
     2161 caccaccata atttcctact cgctcgtgag ctccagaaat gcgattgaac aaagtagttt
     2221 ttccacaatt gggattaccc accaaagcaa catgaataga ttttcctttt tcaagagctg
     2281 atacttttaa agtttcatcg tcaataattc cattaaaatg tatattttgt aaaacactgg
     2341 ttgcttcctg aacagaaatt acctgaatta attcagcttc aattcgccgt aacgaaatat
     2401 tataccctat aatttcatat tctatgggat cttgtaacgg agcacattca ataaccttta
     2461 cttttttacc ttttacaaag cccatttcag cgagtcttct gcgaaaagaa ccatgtccca
     2521 gcacttttac tataattgcc tcatcaccag gcgataggtc agaaagtcgt gtaaaattat
     2581 ttatttcata cactatattc caattttaaa agtctaatcg cattctcaga aaacttatca
     2641 gcgaattttt taatgctata tcagttcctg aagaattgat tacatactga atatctggca
     2701 ttaaactgat atgtctattg atgacaaatt gatatatcat ttctatagtt gcttcgtctc
     2761 ttctattttt tagcgatgca taagctaaag ctaaaccaaa aatatcttct tgtcttttct
     2821 tcccaattcc tttataatta aaccctatac ccaaataaaa gggatttgaa ttttttgatg
     2881 caggactcgc tgcaatttga gaaaatattc caacatgttt attgacagtt tgatcaccaa
     2941 caaaataaaa acctctattc tcctctgtat gttgcatgct atcacgactg ttatgataat
     3001 aagctccaat tttgtaaatt cctgaacgat tttcctggaa gtttttattc cattccattt
     3061 ctgtaatgtt taagaagcca ttttgcttgt ttaatttcca tttcaaatga aaattttcaa
     3121 aaccttctgg cgtaccatcg aaaattgccg ctttccattt gaaatgttgg gtgggttgcc
     3181 attctaaatg aaagcccaat gctgttaacg gaaaaatagg agaaggaatg ttataagcaa
     3241 tggtggaagg aattccgaat gaactattga taaataaact tccatattca ctggcagcgt
     3301 aattcacatt caaatcctgt aaaccaatga tgcttttcac ttttttaaat ttttgttcat
     3361 accagagctc gtacaaataa gtatgattac ttgcttcgat gtttgaaacg ccttgataat
     3421 caccaacaaa atgagccgta ggctgtccac catgagtgtt gaatatattc agaaataagc
     3481 ccccatcttt ccataaatgt gcttttgaag tatcaaaata ggtatttaat gaaattaatc
     3541 ccaaataaga aatacctgtt tttattccac ctgacatatt tgcaaaacca tcaccaatat
     3601 acgaagctga aaaagtaaaa aatgaagttt cattcttttc ttgagcagct aattttataa
     3661 cattatgcaa aagaattatt cctatcaaaa gagtttttcg cactcgatgc cattccattt
     3721 tttctttata attttaatca ttcctaagat ttgatttgcg atgacaaaga tagttattta
     3781 gattgattct aaacaaaatt tcctaaaaaa gttacaaaaa tatctttacc ttacttatta
     3841 acagagaatt gttttta
//
LOCUS       DAOSAS010000150         3840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_15018_length_3840_cov_14.923646, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000150 DAOSAS010000000
VERSION     DAOSAS010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15018_length_3840_cov_14.923646"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLR72_06645"
     CDS             complement(<1..624)
                     /locus_tag="PLR72_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOK36913.1"
                     /translation="MKKVKFLLASLVIIAASSLNAQGFGVQAGYSNSTTFIGEGDTLS
                     NLNGFHVGPIAELTIQGPFSLQYGLFYNYQTKQETYEGVVTAKTSTTFHSVDIPVHLA
                     FTIPTGSPVGVFVFGGPSFNVGISKKTTFKVTSSLGTLEPPTIDYYKTDDNGDSRQSR
                     FDIQMGVGAGIKLQNLQLRLSYDWGLLDTNNSDNYSLTRDELKASIAF"
     gene            complement(773..1780)
                     /locus_tag="PLR72_06650"
     CDS             complement(773..1780)
                     /locus_tag="PLR72_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOK36914.1"
                     /translation="MKVLFIGGTGNLSSACTEIAINKGIEVYHLNRGLTQEIQSTKKV
                     KTIIADIHDTKKVAESLREMTFDSVVDFIAYEPTDIQNDIQLFKDKTHQYVFISTASA
                     YQKPLEKLPITEETPLYNPYWEYSRKKIACEKLLVEEYQKSGFPYTIIRPSHTYGKTT
                     VPLIGGYTVLHRMLKGEPVIVHGDGTSIWTLTYNKDFAVGVIGLLGNMDAIHEAFHIT
                     SDEWLSWNAIYEIMANELGVKPQLVHVPSEIIAKYNQEMGDGLLGDKSHSMIFDNSKI
                     KKFVPEFHPQVSFREGCKEIISWFKENTIHQPIDPEIDETMNNIIHDLKQCQWFPLSK
                     K"
     gene            1938..3170
                     /locus_tag="PLR72_06655"
     CDS             1938..3170
                     /locus_tag="PLR72_06655"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOK36915.1"
                     /translation="MDENQIRKIREDFPILSEKIYNRNLIYLDNAATTQKPRCVVEGM
                     EYAYYHLNANIHRGVHYLSQKATEAYEEARSTVAQFLNAEKREEIIFTRGATESINLV
                     AFSFGEAFCKPGDEIVVSAMEHHSNLVPWQMLCERKRMKLRIIPMNEKGELQLDAFPH
                     LLNEHTKIVAVTHVSNVLGTINPVKEIIEIAHRKNIPVLVDGAQAVPHFAVDVTALDA
                     DFYVFSGHKIYGPTGIGVLYGKEKWLNALPPYQGGGEMIAMVTFEKTTYSEIPFKFEA
                     GTPDYIGSMALAEALLYVQKIGLDVIANYENELLNYAIKRLKEIEGIRFFGTAERKCS
                     VISFLINDIHPYDIGMMLDKLGIAVRTGHHCAQPVIDAFGIPGTIRISFAFYNTKEEI
                     DEFIVALKRIVPMFISRK"
     gene            3218..3646
                     /locus_tag="PLR72_06660"
     CDS             3218..3646
                     /locus_tag="PLR72_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOK36916.1"
                     /translation="MTINEIQEEIIEEFNQFDDWLEKYSLLIDMGNSLEPLDSKYKMP
                     ENLIEGCQSKVWLQAEMKDGKVYFRGDSDAVLVKGIIALLIKVLSGHTPDEILNADLY
                     FIDQIGLREHLSPTRSNGLLAMLKQMKLYALAFKSKGEQY"
BASE COUNT         1234 a          630 c          700 g         1276 t
ORIGIN      
        1 aaaggcaatg gaagctttca gctcatcgcg agtaagacta tagttgtcgc tgttgttcgt
       61 gtccagcaat ccccaatcat aactcaaccg caattgcaag ttctgtaact taatgccagc
      121 gccaacaccc atttgaatat caaagcgaga ttgacgactg tctccgttat catcggtttt
      181 gtaataatca atagttggtg gttccaatgt ccctaatgaa gaagtcactt taaacgtagt
      241 ttttttgctg ataccaacat tgaaactagg acccccaaaa acaaaaacac caacaggaga
      301 acccgtcgga atagtaaatg caagatgtac agggatatca acagaatgaa aagttgtgga
      361 tgtttttgca gtgaccacac cttcataagt ttcctgtttg gtttgatagt tataaaacaa
      421 accatactgt aagctgaaag gaccttgaat tgttagttct gctattggac caacatgaaa
      481 accattcagg tttgacaggg tatcaccttc accaataaaa gtagtagaat tgctgtaacc
      541 tgcttgcaca ccaaaacctt gtgcatttaa acttgaagca gcgataatca ctaaagatgc
      601 caacaaaaat ttcacttttt tcatttgttt tgttttttaa aagttaatgt taaaaatgtt
      661 tattcctgga aagggacaaa aaatataccg ttctttgtaa atagaaaaat actcgatgaa
      721 aaattaataa ttatcagtat ataactcggc aattgaatat ttgttcattc ttttatttct
      781 tggaaagagg gaaccactga cattgcttta agtcatgaat aatattgttc attgtttcgt
      841 caatttcagg atcgattggt tgatgaatgg tattttcttt gaaccaactt ataatttctt
      901 tgcatccttc acggaaagaa acttgtggat ggaattcagg aacaaacttt ttgattttcg
      961 aattgtcaaa tatcatactg tgcgatttgt caccaagtaa accatcaccc atttcttgat
     1021 tatattttgc aataatttct gaggggacat gcaccaactg aggttttacg cccagttcat
     1081 ttgccataat ttcataaata gcattccaac taagccattc atcagaagta atatgaaaag
     1141 cctcgtgaat agcatccata ttcccaagta aacctattac tcctactgca aaatctttat
     1201 tataagttag tgtccagatg gaagttccat ccccgtgaac aatcaccggt tcgcctttca
     1261 acattctatg cagaaccgta taaccaccga ttaaaggaac ggttgttttt ccataagtat
     1321 gtgaaggacg gataatggtg taaggaaaac ctgatttttg atattcttct accaataatt
     1381 tttcacaggc aattttcttc ctcgaatatt cccaataagg gttgtataac ggtgtctctt
     1441 ctgtaatggg aagtttttca agcggttttt gataagctga ggcagtgctt ataaatacat
     1501 actgatgcgt tttatcctta aaaagttgaa tatcattttg aatatctgtc ggttcataag
     1561 caataaaatc aaccacactg tcaaaagtca tttctctgag tgattctgcc acttttttcg
     1621 tgtcgtgaat gtcagcaata atagttttta cttttttagt tgactgtatt tcttgtgtta
     1681 atcctctgtt taagtgataa acttctattc ctttattaat agctatctct gtgcaggctg
     1741 agcttaaatt ccctgttcca cctataaata agactttcat aagcgattat tttttatatt
     1801 tctgaaaatt attacaatta atataacttg ctttaacaat aaagagataa ggaaaagcta
     1861 cacgacaaat ttataagctt ttcttcattt ttttgtactt ttgcaaatta aaatcattaa
     1921 aaatttattt ctatctcatg gatgaaaatc aaatacgcaa aatacgagaa gattttccta
     1981 ttttaagcga aaaaatatac aacagaaatt taatttatct ggataatgca gccactacgc
     2041 aaaagcctcg ttgtgtggtg gagggaatgg aatatgctta ttatcatttg aatgcaaata
     2101 ttcatcgtgg cgttcattat ttaagtcaaa aagctacaga agcttatgaa gaggctcgca
     2161 gcactgttgc tcaatttttg aatgctgaaa aacgggaaga gattattttt acgcgtggtg
     2221 ctactgaatc catcaatctg gttgcttttt cgtttggtga agctttttgc aagccgggtg
     2281 atgaaattgt ggtttcagca atggaacatc attccaattt ggttccctgg cagatgcttt
     2341 gcgaacgaaa aagaatgaaa ctccgcataa ttcctatgaa cgaaaaaggt gaactgcaat
     2401 tagatgcttt tcctcatttg ctcaacgaac atacaaaaat agtagcagtg acgcatgttt
     2461 caaatgtatt gggaacgata aatcctgtta aggaaatcat tgaaatagct caccggaaaa
     2521 atattccagt gttagtggac ggagctcagg cagttcctca ttttgcagtc gatgtaacag
     2581 ctctggatgc cgatttttat gtattttcag ggcataaaat ttatggacca acaggaattg
     2641 gtgttcttta tgggaaagaa aaatggttaa atgctcttcc accttatcag ggaggtggag
     2701 aaatgattgc tatggttact tttgaaaaaa ctacttatag tgaaattccg tttaaatttg
     2761 aagcaggaac acctgattat attggttcga tggcattggc ggaagcattg ctgtatgtcc
     2821 aaaaaattgg tttggatgtc attgccaatt atgagaacga actactaaat tatgcaatta
     2881 aaaggctgaa agagatcgaa ggcattcgtt tttttggtac agccgagagg aaatgttctg
     2941 ttatttcttt tctgataaac gatattcatc cttatgatat tggtatgatg ctcgataaat
     3001 tgggtatagc tgttcgtact ggtcatcatt gtgctcagcc agtgatcgac gcttttggaa
     3061 ttcccggtac gattcgtatt tcatttgctt tttacaacac aaaagaagag attgatgaat
     3121 tcattgttgc attgaaacga attgtaccca tgtttatttc aagaaaataa ttttacaaag
     3181 agaaagaaga aaaagtttta gacaatagaa aataatcatg acaatcaatg aaattcaaga
     3241 ggaaattata gaagaattta atcagtttga cgattggtta gaaaagtatt ctttgctgat
     3301 agatatgggg aattcccttg aaccacttga cagtaaatat aaaatgccag aaaatttgat
     3361 tgaaggttgc cagagtaaag tttggttgca agcagaaatg aaagatggaa aagtatattt
     3421 tcgcggcgac agtgatgcgg tattggtgaa aggaataata gctttgttaa taaaagtgct
     3481 ttcaggacat actcctgatg aaattctcaa tgcagatctt tattttatcg atcaaattgg
     3541 gttgcgtgaa catttgtcac ccacacgttc caatggatta cttgctatgc tcaagcaaat
     3601 gaaattgtat gccttagctt ttaaatcgaa aggcgaacaa tattagttct gagttttgag
     3661 tgttatgttg ttaattgtta atgcctaaat aacgttgacc gttttttaca attttatcat
     3721 ttaaaaagct caagataata attatgattt actaaaattt cagaaatctt ttttctaact
     3781 ttgtcctgta gcatattttt ctgttttgta attatacggc taatatactc attcttaatg
//
LOCUS       DAOSAS010000151         3837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_15039_length_3837_cov_11.521153, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000151 DAOSAS010000000
VERSION     DAOSAS010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15039_length_3837_cov_11.521153"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLR72_06665"
     CDS             complement(<1..353)
                     /locus_tag="PLR72_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36917.1"
                     /translation="MKNYFYKLFFTTVCICSFLFSCTNNVDLSNISTDVQWDGAIAIP
                     IGEANLNIENLLDKIDKNNVLTTEGEEIIYQNEDSTAFRFRDIDLAKNAQSLSTSFPL
                     PPSQTIPANQSANFE"
     gene            complement(573..1988)
                     /locus_tag="PLR72_06670"
     CDS             complement(573..1988)
                     /locus_tag="PLR72_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK36918.1"
                     /translation="MLANYIISEIKAQLPFEPNEQQEALIETLSNFLLSKDKKKIFLL
                     KGYAGTGKTSIISAMVKAMDELYQKNILLAPTGRAAKVIANYAGMPAFTIHKKIYRQK
                     SLADFHFQLSENLHSNTLFIVDEASMISETSSDKIFGSGHLFEDLLEYVYEGNNCSLL
                     LLGDTAQLPPVKEEKSPALDTERLKSYGLNVTEFTLTQVMRQALESGILYNATKLRNH
                     LSKNKVRDFPYLEIKNFKDIVSLPVSEFVNEIQKAYEEVGFDETIVITRSNYRANYYN
                     QGIRNNVLQREEEITNGDLLMVTRNNYFWSQPYEDIDFIANGDILKISRIYHYKEIYG
                     FRFADVTLFSVDENREIDVKLWLDSLQTKNPAQTEEMYKLLMERVAEDYPEIKKRNEK
                     MKRIFENEYYNALQVKFAYAITCHKSQGGQWKRVFIDPGLITEVQINADFYCWLYTAL
                     TRATEKVFLINFSENFVKKNP"
     gene            2152..2736
                     /locus_tag="PLR72_06675"
     CDS             2152..2736
                     /locus_tag="PLR72_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOK36919.1"
                     /translation="MLSHLENYQIILASKSKRRQDLLSGLNIPFGVYPIEVDESFPPE
                     LIGMDIPIYLSKKKFQACTSIMNEKTMIITADTIVWYQGQVFGKPRDREEAKSMLITL
                     SGKTHDVITGVTIATFRKQRTFGVASEVRFARIAEEEIDYYLDHYDPYDKAGGYAVQE
                     WIGYVGVEHINGSYYNVMGLPVQRLYNELKNWKE"
     gene            complement(2764..3837)
                     /locus_tag="PLR72_06680"
                     /pseudo
     CDS             complement(2764..3837)
                     /locus_tag="PLR72_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696873.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
BASE COUNT         1328 a          592 c          617 g         1300 t
ORIGIN      
        1 ttttcaaaat tagctgattg atttgctgga atagtttgtg aaggtggtaa aggaaaagaa
       61 gtacttaaag attgggcatt ttttgccaaa tcaatatccc tgaatcggaa agctgtgcta
      121 tcctcgtttt gataaataat ttcttctcct tccgtagtga gaacattatt tttatcaatt
      181 ttatccagta aattttctat attcaagtta gcttctccaa ttggaattgc tatcgcccca
      241 tcccattgta catcagtaga aatattagat aaatctacat tgttggtaca agaaaacaaa
      301 aaggaacaga tacaaacagt tgtgaaaaat aatttgtaga agtagttttt catgatgtct
      361 ggtttttgag aaaattaaaa atttaagaat aatggttgat gaataattta aatccaaaaa
      421 gagagaacgg aacttaaaaa tttttaattt tctttgtttt taaataaaaa taagcaaaaa
      481 aaattaaaaa gaataatgga aatcaatatt aaattaattg tgtgctaaaa tcagcttcaa
      541 atgtaataat tattttgtta aaaaaaatat ttttatggat tttttttgac aaaattttca
      601 gaaaaattaa taagaaaaac tttttctgtt gctctggtta aagcagtgta aagccagcaa
      661 taaaaatcag cgtttatttg aacttctgta ataagtcccg gatcaataaa aacccttttc
      721 cattgacctc cttgtgactt atgacaggtg atggcataag caaatttaac ctgtaaagca
      781 ttgtaatatt cattttcaaa aattcgtttc attttctcgt ttcgtttttt tatttccgga
      841 taatcttcag caactctttc cattaagagt ttgtacattt cttctgtttg agcagggttt
      901 ttggtttgta aactatccag ccataatttt acatctattt ctcgattttc atcaacagaa
      961 aataacgtaa catcagcaaa acgaaagcca tatatttctt tgtaatgata aattctactg
     1021 attttcaata tatcaccatt ggcaataaaa tcaatatctt cataaggttg tgaccagaag
     1081 taattatttc gcgttaccat tagcaaatcg ccgtttgtaa tttcttcttc tctttgtaaa
     1141 acattgtttc tgatgccttg attgtagtaa ttggctctgt aattggatcg tgttatgaca
     1201 atagtttcat caaatccaac ttcttcatag gctttttgaa tttcattgac aaactctgat
     1261 acaggaagcg aaacaatatc tttaaaattt tttatttcca gatatgggaa atctcttact
     1321 ttatttttac tcaaatgatt tctcaatttt gtggcattgt ataatattcc actttccaga
     1381 gcttgacgca ttacctgtgt gagcgtaaat tccgtgacat tcaaaccata acttttcagt
     1441 ctttctgtat ctaaagcagg acttttttct tcttttacag gtggaagttg agccgtatca
     1501 cccagcagta ataaactgca attgtttcct tcataaacat attccagtaa atcttcaaat
     1561 aaatgtccag aaccaaaaat tttatcactg gaagtttcag aaatcatcga agcttcatct
     1621 acgataaaaa gggtatttga atggagattt tcagaaagtt gaaaatgaaa atcagcaagt
     1681 gatttttgtc gataaatttt tttgtgaatc gtaaaagctg gcataccagc ataattggca
     1741 atcactttgg cagctcttcc agttggtgcc agcaaaatgt ttttttgata aagctcatcc
     1801 atggctttta ccatagccga aataatagaa gtttttcccg ttcctgcata tccttttaat
     1861 aagaaaattt ttttcttatc ctttgaaagc aggaaattac ttagtgtctc aataagtgct
     1921 tcttgctgct cgtttggctc aaaaggtaat tgagctttaa tttctgatat aatgtaatta
     1981 gctaacatat ttacaaatat atcaaggata ttttacattt tatacgaaaa atttctattt
     2041 ttgtaaaaat aaacacttta ttcaagtgga attgtttaag ggtttgtttt ttcattcttt
     2101 agtctgaaaa atatttattt gcaaagtgtt ttaataaaaa actaagttat gttgctttca
     2161 catcttgaaa attaccaaat tattttagct tcaaaatcga aaagacgtca agatttatta
     2221 agtggactga atataccttt tggtgtatat cctattgagg ttgatgaaag ttttccacca
     2281 gaattaattg gaatggatat tcctatttat ttatctaaaa agaaattcca ggcttgtact
     2341 tctattatga atgaaaaaac catgattatt actgctgata ctattgtctg gtatcaaggt
     2401 caggtatttg gcaaaccaag agatagagaa gaagcaaaaa gtatgcttat aaccctatca
     2461 gggaaaactc atgatgtaat tacaggtgtc accattgcta cttttcgtaa acaacgaact
     2521 tttggtgttg cttccgaagt aagatttgcc agaattgcag aagaagaaat tgattattat
     2581 ctcgatcatt atgatcctta tgacaaagct gggggttatg ctgttcagga atggattggt
     2641 tatgtaggag ttgagcatat caatggctcg tattacaatg taatgggact tcctgttcaa
     2701 cggctgtata atgaattgaa aaactggaaa gaatgaaaat tttcccgaga aagtgatttt
     2761 ttctcattta aagaagctga taaacagcag aacattcaag attgttacta ttattcctaa
     2821 taaataaaga atgattttat gaaataagtc gtttttgtat ttccccatta ctttttcaga
     2881 ggaagtaaga taaacttgcg aaaaaacggt aaaggggagc tggatactta aaatcatttg
     2941 agatataatt agtcctttaa aaggatcacc aatcaaaaag atgataatca ttgccagtat
     3001 gagagaaaaa gcaactccca aacgagaatg attatcttta atgtcataag gttcttgata
     3061 cattcctgaa aaaatagtac cgccagccat tcctgatgtg atgctggatg aaatgccaga
     3121 aaatagtaaa gcaactgcaa aaaccactgc tgcattattt cctaaaagcg gtgttaacaa
     3181 ttgatgagtt tgctgtaatt cagtaacagg agttttagtt tgataaaaag tgctagcagc
     3241 gagtaatata atagcactgt taatagccca tccgatcaac atagaaacaa gcgtgtctaa
     3301 aaattcatat tttaattgcc ttttgataac attttcattt tcaagattcc attgacgact
     3361 ctgaattatt tcagaatgta aaaataagtt gtgcggcatc accacagctc ccaatacact
     3421 cataataatt attattgaac cttctggaaa tgaaggcgtt acacaacctt ttgctgcctg
     3481 tgaccaatca acttttacta acgacaattc acacaaaaaa gataatccaa taatggacac
     3541 gaaagcaatt atccattttt caatggcttt ataagagttg gtaaaaagca taatgagaac
     3601 aaaaactgtt actaaaattg taccagctgg aattaaaata tgaaacaaca tgttcaaagc
     3661 aatacttccg cctaaaattt cagcaaggga agtagaaacg gaagcacaca tggcagtaat
     3721 gagcaaaaac tttgaataaa cgggcttcat atattttgaa atggcttccg caagacaaag
     3781 acctgttact attcacaaga tgtgcaacat tgtgccgaaa aaaattagca taatggt
//
LOCUS       DAOSAS010000152         3756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_15496_length_3756_cov_11.868684, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000152 DAOSAS010000000
VERSION     DAOSAS010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15496_length_3756_cov_11.868684"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..2021
                     /gene="ligA"
                     /locus_tag="PLR72_06685"
     CDS             21..2021
                     /gene="ligA"
                     /locus_tag="PLR72_06685"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOK36920.1"
                     /translation="MSIKEQIEALRKELEQHNYYYYVLSQPIISDFEYDMKLKQLQEL
                     EEKYPEYYDPYSPTQRVGSDLTEGFEQAYHAYPMLSLSNTYTEEEVKEFYERVSRTLN
                     EPFELVGELKYDGVSISITYENGVMIRAVTRGDGEKGDVVTANVKTIKSIPLRLRGDY
                     PSKFEIRGEVLMSWEVFDKLNKEREAQEEPLFANPRNATAGTIKLLSPAIVASRNLDA
                     YFYYVLGEELPYRTHYDNLQAAKTWGFKISSDMKVCKNLEEVFDFIHYWDVERRKLPI
                     PTDGVVLKVNSLDQQRKLGATAKFPRWAIAFKFQAEKALTKLLSVSYQVGRTGAVTPV
                     ANLEPVVLSGTTVKRASLHNADIIESLDLHIGDMVYVEKGGEIIPKITGVEKSARIQV
                     GEKVQFIKNCPECGTPLIRYPGEAAHYCPNENGCPPQIKGKIEHFVSRKAMNIEGLGT
                     ETINLLYNKGLLRNIADIYDLKFSDLVNLERMGEKSANNLLNSIEKSKQVPFERVLFA
                     LGIRFVGETVAKKLAAHFKSIDALQHATYEELLEVEEIGDKIAQSVIQFFSDPKNLEI
                     IQRLKAAGVQMSVSEENQLLKGNELEGKSIVISGVFKLHSRDEYKKMIEEHGGKNVSS
                     VSSKTSFILAGDNMGPEKLKKAQQLGIPLIDESTFLAMINEK"
     gene            2054..2617
                     /locus_tag="PLR72_06690"
     CDS             2054..2617
                     /locus_tag="PLR72_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36921.1"
                     /translation="MIVDKIAKYQLKKEVKRYNLSTAREKRFINYDQARNFLLIFESD
                     ENEKNLPIKKIIEKLKSDDKKVTAIGYLNKKKLCTPFLLDYKIFNSRQTNFFKKPNEF
                     ILKELRNYQFDIVIDLSFHNVIPLQYVVLYVNAFLKVGLKNADLSIYDFIFNITNQFK
                     EELSFFDNINNIFNQIIFYLKNIQSSD"
     gene            2654..3541
                     /gene="dapA"
                     /locus_tag="PLR72_06695"
     CDS             2654..3541
                     /gene="dapA"
                     /locus_tag="PLR72_06695"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOK36922.1"
                     /translation="MNMYNKLTGMGVALVTPFKNDDSIDFEAFGRLIEYIIKNGADYL
                     VVCGTTAETPTLTEDEKDELARFAVQQNAGRLPIVLGIGGNNTKAVVEKIKTTDLSRI
                     DAILSVVPYYNKPTQEGLYQHFASIAKASSLPIILYNVPGRTSVNMTAETTLRLAKDF
                     PNICAIKEASGNFVQIGEIIKNKPKDFLVISGDDGVAYPLITMGGVGVISVIGNAFPA
                     EFSKMVHLALQGDYASARRIHYRFTELFELLFVEGNPAGVKSMLAIMGMIENKLRLPL
                     VPASAKTYEKIQSAIDQLN"
BASE COUNT         1321 a          571 c          718 g         1146 t
ORIGIN      
        1 aacacatttt gattatggaa atgagcatta aagaacagat agaagcactg cgtaaggaac
       61 tcgagcaaca taattattat tattatgtac tttctcagcc gattatttca gatttcgaat
      121 acgatatgaa actgaaacaa ttacaagagc tcgaagaaaa atatcccgaa tattacgatc
      181 cttattcacc aactcaacgg gttggcagtg atttgactga aggttttgaa caggcttatc
      241 acgcttatcc catgctttcg ttgagtaata cttacacaga agaagaagta aaggaatttt
      301 acgagcgagt ttcaagaaca ttaaacgaac cttttgaact ggtaggagag ctgaaatatg
      361 atggtgtttc aatttccata acttatgaaa atggtgtgat gattcgtgct gtgacacgtg
      421 gtgatggcga aaaaggtgat gttgtaacag ccaatgtgaa aactattaaa agtatcccat
      481 tgcgattgcg aggtgattat ccatcaaagt ttgaaattcg tggagaagtt ctcatgtctt
      541 gggaagtttt tgataaattg aataaggaaa gggaggccca agaagaacca ctttttgcca
      601 atcctcgaaa tgctactgct ggcactataa aacttttaag tccagcaatt gttgcttctc
      661 gaaacctgga tgcttatttt tactatgtgt tgggagaaga attgccatac cgaactcatt
      721 acgataatct tcaagcggct aaaacatggg gatttaaaat ttcaagtgac atgaaagtct
      781 gcaaaaatct tgaagaagta tttgatttta ttcattattg ggatgttgaa cgacgaaaat
      841 tacctatccc aactgatggt gttgtgctaa aagtaaattc tttagatcaa caacgaaagt
      901 taggagctac tgcaaaattt cctcgctggg caattgcttt taagtttcag gcggaaaaag
      961 cactgacaaa acttttatcc gtttcgtatc aagttggacg tacaggtgca gtaactcctg
     1021 tagcaaatct ggaacccgta gtactttcgg gaacaacggt aaaacgtgct tcgttacaca
     1081 atgcagacat tatcgaatca ctcgatttac atatcggcga catggtatat gttgaaaaag
     1141 gcggagaaat tatacctaaa atcacaggtg tagaaaaaag tgcaagaata caagtcggag
     1201 aaaaggttca atttattaaa aattgtcctg aatgtggaac gcctctgatt cgttatcctg
     1261 gtgaagcagc tcattattgc ccgaacgaaa atggttgtcc gccacaaatc aaaggaaaaa
     1321 tcgaacattt cgtcagccga aaagcaatga acattgaagg attgggaaca gaaaccataa
     1381 atctgcttta taacaaaggt cttttgcgaa atatagccga tatttatgat ttaaaatttt
     1441 ctgatttagt gaatttggaa cgaatgggag aaaagtcagc taacaatctt ttgaattcta
     1501 tagaaaaatc taaacaagtt ccttttgaaa gagttttgtt tgctctggga attcgttttg
     1561 taggcgaaac agtagctaaa aaattagccg ctcatttcaa atctatcgat gctttgcaac
     1621 atgccaccta cgaagaactc ctggaagtag aagaaattgg cgacaaaata gcacaaagtg
     1681 tgatccaatt tttttctgat cctaaaaatc tggaaattat tcaacgactc aaagctgctg
     1741 gtgttcaaat gagtgtttct gaagaaaatc aattattgaa aggaaatgag ttggaaggga
     1801 aatctattgt gattagtggg gttttcaaac ttcattcacg agatgaatac aaaaaaatga
     1861 ttgaagaaca tggtggaaaa aatgtgagca gcgtttcttc aaaaacatct tttattctgg
     1921 caggagataa tatgggaccc gaaaaactca aaaaagctca acaactgggt attccattga
     1981 ttgacgaaag tacgttttta gcgatgatca atgaaaagta ataataaact ttgtaaatta
     2041 aggttttaat tttatgatag ttgataaaat agctaaatat cagttaaaaa aagaagtaaa
     2101 aaggtataat ttatctaccg ctcgagaaaa acgttttata aactatgatc aagcacggaa
     2161 tttcctgttg atttttgaaa gcgatgaaaa tgaaaaaaat cttcctatca aaaaaattat
     2221 agaaaaatta aaatctgatg acaaaaaagt aactgctatt ggttatttga acaagaaaaa
     2281 actctgtaca ccatttttgc ttgattataa aatttttaat tctcgacaaa ccaatttttt
     2341 taaaaagcct aacgagttta ttttgaaaga attgagaaac tatcagtttg atatagtaat
     2401 tgatttatca tttcataatg tcattccttt gcaatatgtg gtgctttatg tcaatgcttt
     2461 tcttaaagta ggattgaaaa acgctgattt gtcaatttac gattttattt tcaacattac
     2521 gaatcaattt aaggaggaat tatccttctt tgacaacata aataatattt ttaatcaaat
     2581 aatattttac ttaaaaaata ttcaatcttc tgattaaaag cgtaattttg tgatgaatta
     2641 aaatatattt tctatgaaca tgtataataa attaacggga atgggagtag ctttggtaac
     2701 tccttttaaa aatgatgatt caattgattt tgaggctttt ggaaggttaa tagaatacat
     2761 aattaaaaat ggtgctgatt atctggtagt gtgtggaaca actgctgaaa cacccacctt
     2821 aactgaagac gaaaaggatg aactggctcg ttttgctgtt caacaaaatg ccggacgatt
     2881 accaattgtg ctgggcatcg gtggaaataa tacaaaagct gtagtagaaa agattaaaac
     2941 tactgattta tcaagaattg atgccatatt atctgttgtt ccttattata ataaacctac
     3001 acaggaagga ctttatcaac attttgcaag tatagcaaaa gcttcgtcac ttcccatcat
     3061 tctctacaat gttccaggca gaacaagcgt aaatatgaca gcagaaacta ctttgcgttt
     3121 ggcaaaagat tttccaaaca tttgtgcaat aaaagaagct tcaggtaact ttgtccagat
     3181 tggtgaaatt ataaaaaaca agcctaaaga ttttttggta atttcaggcg atgatggcgt
     3241 tgcttatccg ctgattacta tgggaggtgt tggtgtcatt tcagttatcg gaaatgcttt
     3301 tcctgctgaa tttagtaaaa tggtacatct ggctttacag ggcgattatg caagtgcacg
     3361 acgaattcat tatcgtttta cggaattatt tgaattactt tttgttgaag gcaatcctgc
     3421 tggtgtgaaa agtatgttgg caattatggg aatgatagaa aataaattaa gattgcctct
     3481 tgttcctgct tccgcaaaaa catatgaaaa aatacaatca gctattgatc aattgaacta
     3541 atatgctttg ctgacaaaat tattgattgt actatttgct tgttagtgaa acgtctcata
     3601 atctttataa gacatcaccc gctcccgccg atttgtaatc ggtggtttgg tctgtagagc
     3661 aagaaaggta gaaaagcaaa tgtagaaaag caaatgtaga aaagaataca agagcaccaa
     3721 aagaaagaag aaaaaccctg ttcgacgtag cctcaa
//
LOCUS       DAOSAS010000153         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_17222_length_3485_cov_14.283673, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000153 DAOSAS010000000
VERSION     DAOSAS010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17222_length_3485_cov_14.283673"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1129)
                     /locus_tag="PLR72_06700"
     CDS             complement(98..1129)
                     /locus_tag="PLR72_06700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK36923.1"
                     /translation="MQVSIVILNWNGEKYLSQFLPILIEKTQLSDVEIVVADNGSTDN
                     SVKLLQKNFPSVKLLLFDKNYGFAGGYNKALNQLQSDYFVLLNSDMEVTDNWLQPMLE
                     YMKEHENVAACQPKIHSYFRRSYFEHAGAAGGFIDPYGFAFCRGRIFGRVEEDRGQYD
                     DIKDIFWASGACMMVRAELFSKVGGFDDEFFAHMEEIDLCWRFNSRGYRVICVPQSVV
                     YHIGGGTLNAEHPHKTYLNFRNNLLLLYKNLPNHQLKNIMQRRTIFDYLAAFQLFMSG
                     KFKNAQSVLRARKDFKRMKNRFEGKRKENILYSTVSDLPTIYPKSIVIEYYLKGKKKF
                     SDLYFPDLE"
     gene            complement(1155..2045)
                     /locus_tag="PLR72_06705"
     CDS             complement(1155..2045)
                     /locus_tag="PLR72_06705"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOK36924.1"
                     /translation="MFYLVYPFFWLLSLSPLNFLYCLAKPFYFFIYYVVRYRRKVVRT
                     NLLHSFPDKSLHELIKLEKDFYHFFSELLMEIVKLIHMSDEELSKRVTFVDSEQFFEL
                     CSQGRYPLLMLGHYGNWEWILSISLLMPKNTFAFTVYKRLKNKKFDQFMLNLRTKRGA
                     VMVEKRSLLRSMIRWAKEGKLGGYGIIADQSAGGKNIQNWTTFLHQETAILPGTEELA
                     RKFNYPVFYARVSRPKRGYYQCQIIPIALEPQKTSENEISQRFMHLLEEDILSHPQYW
                     LWTHRRWKHQRTNQPPINES"
     gene            complement(2153..2491)
                     /locus_tag="PLR72_06710"
     CDS             complement(2153..2491)
                     /locus_tag="PLR72_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36925.1"
                     /translation="MKETEENLLEYSEDEAVNFIFNSFPEEAKKRINKDIINYVLDVV
                     YDFYEEKGLLDEEDEEVTESFIDEEELFEYILKAAKKDKINLTGEDIELILDAEFEYG
                     KSIGIYKEEE"
BASE COUNT         1245 a          610 c          424 g         1206 t
ORIGIN      
        1 aattacagga ggaaaaataa aaaataacgt aacttacaag cagtataaaa cttctcgata
       61 ataacgggtt tgcaagcgat tcgtcctcta tcaaagttta ttctaaatca ggaaaataaa
      121 gatcggaaaa tttctttttc ccttttaaat aatattctat cacaatactt tttggataaa
      181 tcgttggcaa atcagaaaca gtggaatata aaatattttc ctttcgtttc ccttcaaaac
      241 ggtttttcat tcgtttaaaa tcttttcttg cacgcaaaac actttgtgca tttttaaatt
      301 ttcctgacat aaacaattga aaagcagcca gataatcaaa aattgttcgt cgttgcataa
      361 tattttttaa ctgatgattg ggcaaatttt tatataaaag caataaattg ttcctgaaat
      421 tcaaataagt tttatgcgga tgttcagcat ttaacgttcc acccccaata tgataaacca
      481 cactttgtgg aacacaaatt acccgataac ctcgactatt aaaccgccaa cataaatcaa
      541 tttcttccat atgagcaaaa aattcatcat cgaatccgcc aaccttcgag aaaagttctg
      601 cccttaccat catacaggca cctgaagccc aaaaaatatc tttaatatca tcatattgtc
      661 ctctgtcttc ttctactctt ccaaaaattc gtcctcgaca aaaagcaaaa ccataaggat
      721 ctatgaatcc tcccgcagct cccgcatgct caaaataaga acgacgaaaa taagaatgaa
      781 tcttaggttg acaagcagca acattttcgt gttctttcat atattcaagc atcggctgta
      841 accaattgtc agtcacttcc atatcagaat tcaataggac gaaataatcg ctttgtagtt
      901 gatttaaagc tttattgtat cccccagcaa atccataatt tttatcaaat aaaagtagtt
      961 taactgatgg aaaatttttt tgcaataatt ttacagaatt atctgttgaa ccattatcag
     1021 ccaccacaat ttctacgtca gataattgtg ttttttcaat taaaataggc aaaaattgcg
     1081 ataaatattt ttctccattc cagttcaaaa taacaatgga aacttgcata aacaactaat
     1141 ttttaaataa taagttaaga ttcatttatt ggaggctgat tcgttctttg atgtttccag
     1201 cgtctgtgtg tccataacca atattgcgga tgggaaagaa tatcttcctc cagcaaatgc
     1261 atgaaacgtt gcgaaatttc attttcagaa gttttttgcg gttctaaggc tataggaata
     1321 atctgacatt gataataccc tcgtttagga cgactaaccc ttgcataaaa aacaggataa
     1381 ttaaatttgc gtgctaattc ttcagttccc ggcaaaatag ctgtttcttg atgcaaaaaa
     1441 gttgtccagt tttgaatatt tttcccccca gcactttgat ccgcaattat tccataacct
     1501 cctaattttc cctcttttgc ccaacgaatc atgcttcgca ataaacttcg tttttctacc
     1561 attactgccc ctcgtttggt acgcaaattc aacataaatt gatcaaattt tttgttcttc
     1621 aaacgtttat aaactgtaaa agcaaaggtg ttttttggca ttaacaaact gatactcaat
     1681 atccattccc aattaccata atgtcccaac atcaagagag gatacctgcc ttgactgcaa
     1741 agttcaaaaa attgttcgct gtccacgaaa gtaactcgtt tactcaattc ttcatcgctc
     1801 atgtgaatga gcttaacaat ttccattaat aattcagaaa aaaaatgata aaaatctttt
     1861 tccagtttga ttagctcgtg taatgattta tctgggaaag aatgaagaag atttgttcgt
     1921 actacttttc gcctgtatct gactacataa taaataaaaa aataaaaagg ctttgccaga
     1981 cagtacaaaa aatttagtgg cgataatgaa agcagccaga aaaaaggata aacaaggtaa
     2041 aacataatat tgttaaaaaa caaaaaatac agaagataaa aaaacaccaa gaaagaataa
     2101 aaatcctgat gctttcaaaa tttacaaaaa gaataaaata atctgctaat ttttattcct
     2161 cttctttata aatgccgatt gattttccat attcaaattc agcatccaaa attagctcaa
     2221 tatcttctcc agtaagatta attttatctt ttttagctgc ctttaaaata tattcaaaca
     2281 attcttcttc gtcaatgaag ctttccgtta cttcttcatc ttcttcatcc aaaagacctt
     2341 tttcttcata aaaatcatat actacatcca gcacataatt aataatgtcc ttattaattc
     2401 gttttttagc ttcttcagga aaactattaa aaataaaatt cacggcttcg tcttcactgt
     2461 attctaacaa attttcttct gtttctttca taatggttca tttttaagta aaaaattcaa
     2521 ttttcttttt tgttcctcaa aagtaatact tttaagatta caaatgttta taaattaaat
     2581 atttttcaat aaattaaact aaataatatt tttgtttagt gtgtaaataa aatctggtgg
     2641 aaataaaaat tgataaagac attgatagaa gtaatttgtt ttcatttaca cccatttgaa
     2701 ttcagcattt ataatttttg acctataaaa aaacagctta ttttttgatc tatataccta
     2761 attttttaca tttttctcct tatttatttc actataaatc aaaattttat tacaaaaata
     2821 tttgtttact cataacccgg agcctgtatc acgcaaacta gcaattaaaa tttcgttcct
     2881 gacactcaat tttagcataa atgattttat tctactcaaa tttcctttcc aacagaacca
     2941 cgcaaaaaat tatacaaatt ttcatataaa catttacaag ccagtgtttt tcattgttgt
     3001 ttactatttt ttataaaaat atttacagac gaaaaccata gagaataaaa aaggaggtca
     3061 tttaaaaaaa cagtattctc ttaccaacat aaatttatca actatcctac taaagggtag
     3121 gaaataatgg cagctattaa caaaatatcc catagtaata aattaccctg ataaaaatta
     3181 ttcttgtcta caatgaataa tcccgaggca agcctcgagg aattcttttg attaaatttg
     3241 attgctgaat ccagcacatt aatatttaaa tttcttggct tcaaatgaat ttttattacg
     3301 aaatttgtct aaccaaaaag ttgcatttgt aacttgaatt cagcaccaaa aaattaaatt
     3361 tttgacttaa atttagtaga aaaataatta attatttgat attcaaaaac taataaatta
     3421 tgatgttgag tttttgttga gtttaaattt atgattcatt gagtttttgt gtggtaaaaa
     3481 atttt
//
LOCUS       DAOSAS010000154         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_18569_length_3296_cov_11.544276, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000154 DAOSAS010000000
VERSION     DAOSAS010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18569_length_3296_cov_11.544276"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..468
                     /locus_tag="PLR72_06715"
     CDS             103..468
                     /locus_tag="PLR72_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36926.1"
                     /translation="MKSAKPIILSTGLLEPNTPKTNGMMERANGIIKKENILKDNYVN
                     KEEMNDALMAFFVYYMLYRRQGGLRKELNVKTPFQAVEKWFMLKPEIFKQNPPEFKNK
                     IILLKLIENPSFHKQPCET"
     gene            complement(490..990)
                     /locus_tag="PLR72_06720"
     CDS             complement(490..990)
                     /locus_tag="PLR72_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP19 family protein"
                     /protein_id="HOK36927.1"
                     /translation="MIQIKENILKSAEQKGNQQFLQAIIDAYLEALDGQLTAKNMDLF
                     NGWQHSLLAWYYFSTEVNQGGFIQLIQNGYGSYIFDNPFAKAMRLFGAEELSKIVYKA
                     KKIYDIHRDKLEHETTDEEFMTMYLEFEQFDELEEKYFDIEDQQIDAIANYVKTHLEN
                     FIEIIP"
     gene            complement(997..>3296)
                     /locus_tag="PLR72_06725"
     CDS             complement(997..>3296)
                     /locus_tag="PLR72_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOK36928.1"
                     /translation="ENENNINLNSLTTLDVGENIIYLQDFILNTALIDSITSSSNYLK
                     LSLTTDKKIALATVFPEMETFVDVQVWIDSVPYSIPCRKTDKIDYLFTFHSQGKKYKR
                     IQISGQMNDWVPEMTPDLQLNDSGYYQVKLHLSPGIYFYQLVVDNKSIPDPNNPLKVD
                     NGGGNFNSILRVEGNEKFFPVLYTEEFSNNKIKLAYRNKVKDVFVYWQNYRLPSLFIK
                     KNEDKIIFDIPREAFVVNRSFLRIWASNELGVSNDILIPLQNGKVVTDAKDINRFDKQ
                     AQIIYFLLVDRFKNGNSKNDFPLNRPDVHPKVDFQGGDLAGVQQKIEDQYFNQLGINT
                     LWISPLNQNPTEPYGYYAPAKTKFSGYHGYWPVSSSKVDFRFGSNDEFKNLVTLAHEN
                     ELNILLDYVAHHVHEEHPLYEAHPEFFTSLYLPDESLNVARWNDQRLTTWFDTFMPTL
                     NTKSPTVINMMTDSALFWIKEFNLDGFRHDACKHISEDFWRALTLKIKIQNHGKNLYQ
                     IGETYGSPKLISSFLSTGMLDGQFDFNVFDEATFTFTGIAGNDMQRLSDILQMSLKEY
                     GNHNLMGYISGNHDRPRFMAYASGDLKFGEDTKLAGWKRDIGITDSTAYDKLFLMHVF
                     NFTIPGIPVIYYGDEIGMTGANDPDCRRMMYFDNWNRREKNLWQQVSTLTHLRTSHPV
                     FLYGDFVQLKVDKNSWIYARKYFDKIALVFINNSPSTKKFEINLPDYLIPVQSFNSVF
                     QYSFSLINQRVLIELPRYSAEILIN"
BASE COUNT         1146 a          576 c          489 g         1085 t
ORIGIN      
        1 aaaagagcat ccgaatgaaa acttgattaa ttttgaaaat taaaaacaaa aaatcatgca
       61 acaagtttat cattcgaatg acgtaacaaa tgtacacatt agatgaagtc tgcaaaacca
      121 atcatattga gcacaggctt acttgagccc aataccccaa aaacgaacgg catgatggaa
      181 agagcaaacg gaattattaa aaaggaaaac attcttaaag acaattacgt aaataaagaa
      241 gaaatgaatg atgcgctgat ggcttttttt gtctattata tgctttacag aagacagggt
      301 ggactaagaa aagaactaaa cgtgaaaact ccttttcaag cagttgaaaa atggtttatg
      361 ttaaaacctg aaatttttaa acaaaatcct cctgaattta aaaataaaat tatactttta
      421 aagcttattg aaaatccaag ttttcataaa caaccttgtg aaacttgaca atttacaata
      481 aaaaactttt tatggaataa tttcaataaa attttccaga tgtgttttta cgtaatttgc
      541 tatggcatct atttgttgat cttcaatatc aaaatatttt tcttccagct catcaaattg
      601 ctcaaattca agatacatcg tcataaattc ttcatcagtt gtctcgtgtt ctaatttatc
      661 tcgatggata tcataaattt ttttggcttt gtaaacaatt ttagataatt cctccgcacc
      721 aaacaatctc attgctttgg caaatggatt gtcaaaaata taactcccat aaccattctg
      781 aataagttgt ataaagccac cttgatttac ttctgtcgaa aaataatacc atgcaagtaa
      841 ggagtgttgc cagccattga ataagtccat gtttttagca gtcaattgtc catctaaagc
      901 ttccaaataa gcatcaataa ttgcctgcaa aaattgctga tttccttttt gctcagcaga
      961 ttttaaaata ttttccttta tttgaatcat agttttttag ttgattaata tttctgcact
     1021 atagcgaggc aattctatta agaccctttg attgatcaat gaaaatgaat attgaaatac
     1081 cgaattaaat gactgtacag ggattaaata atctggtaaa tttatttcaa atttttttgt
     1141 cgaaggtgaa ttgttgataa aaacaagtgc aattttatca aaatattttc gtgcataaat
     1201 ccaactgttt ttatctacct tcaactggac aaaatctcca taaagaaaca ccggatgact
     1261 ggttcttaaa tgtgtcagcg ttgaaacttg ttgccataaa tttttttctc ttcgattcca
     1321 gttatcaaaa tacatcattc ttctgcaatc cggatcattg gcaccagtca tgccaatttc
     1381 gtctccataa taaatgaccg gtataccagg aattgtaaaa ttgaaaacat gcatcagaaa
     1441 caatttatcg taggcagttg aatcagtaat gccaatatca cgcttccaac cagctaattt
     1501 tgtatcttca ccaaatttta aatcaccaga tgcataagcc ataaaacgtg gacgatcatg
     1561 attaccggaa atatatccca ttaaattatg attgccatat tcttttaaac tcatttgtaa
     1621 aatatcgctt aatcgctgca tatcattccc agcaattcca gtgaaagtaa aagttgcttc
     1681 atcaaataca ttaaaatcaa attgtccatc cagcatacca gtacttaaaa agctgctgat
     1741 taattttgga ctgccatatg tttcgccaat ttgatacaga ttttttccat gattttgtat
     1801 ttttattttc aaggtaagtg cccgccaaaa atcttcagaa atatgtttac aagcatcatg
     1861 acgaaagcca tcgagattaa attctttgat ccagaacaaa gctgaatcgg tcatcatatt
     1921 aatgacagtt gggctttttg tgttcagcgt tggcataaaa gtatcaaacc acgtggttaa
     1981 tcgctgatcg ttccagcggg cgacgttcaa actttcgtca ggcaaataaa gagaagtgaa
     2041 aaattcagga tgagcttcat acagcgggtg ttcttcgtga acgtgatgag caacataatc
     2101 aagtaaaata ttcaactcgt tttcatgagc aagtgttacc aaatttttaa attcatcatt
     2161 agagccgaag cgaaaatcga cttttgaaga tgacacaggc caatatccgt gatatcctga
     2221 gaatttagtt ttagccggag cataatatcc atatggttcc gtaggattct gattgagcgg
     2281 tgaaatccaa agtgtattga tgcctaattg attaaaatat tgatcttcaa ttttttgttg
     2341 taccccagct aagtctccac cttgaaaatc cacttttgga tgcacatcag gacggtttaa
     2401 cgggaaatca tttttagagt tgccattttt aaatctatcc actaaaagaa aataaataat
     2461 ttgtgcttgt ttatcgaaac ggttaatatc tttggcatca gtcacaactt ttccattttg
     2521 aaggggaata agaatatcat tacttacacc gagttcattg cttgcccaaa tacgtaaaaa
     2581 agaacgattc actacaaagg cttcccgagg aatatcgaaa ataattttat cttcattctt
     2641 ttttataaat aacgagggca aacgataatt ttgccaataa acaaacacat ccttcacttt
     2701 gtttcgataa gctaatttga ttttattatt cgaaaactct tctgtataaa ggacaggaaa
     2761 gaatttttca tttccttcaa ctcttaaaat agaattaaaa ttcccccctc cattatctac
     2821 ctttaaagga ttgtttggat ctggaataga cttattatca accactaact gataaaaata
     2881 aattccagga ctcaaatgta attttacctg ataatatcca ctatcattca attgcaaatc
     2941 aggtgtcatc tctggaaccc aatcattcat ctgtcctgaa atctgaattc ttttgtattt
     3001 tttcccttgc gaatgaaaag taaaaaggta atcaatttta tctgtttttc gacatgggat
     3061 tgaataagga acactatcta tccacacctg aacatcaaca aatgtttcca tttcaggaaa
     3121 aacagtagcc aaagctattt ttttatctgt tgtaaggctt aattttaaat aattagaaga
     3181 acttgtaata gaatcaatta aggctgtatt taaaataaaa tcctgcaaat aaataatatt
     3241 ttcaccaaca tcaagggtcg taagagaatt taggttaata ttattctcat tttctg
//
LOCUS       DAOSAS010000155         3214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_19240_length_3214_cov_14.422286, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000155 DAOSAS010000000
VERSION     DAOSAS010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19240_length_3214_cov_14.422286"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1152
                     /gene="trpS"
                     /locus_tag="PLR72_06730"
     CDS             157..1152
                     /gene="trpS"
                     /locus_tag="PLR72_06730"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOK36929.1"
                     /translation="MEIVLSGIRPTGKLHLGNYFGALRNFVHLQNEYDCYFFIADYHS
                     LTTHPTPHNLFGNVREVLVEYLAAGLDPEKATLYIQSDVPQVTELYLFLNMNAYIGEL
                     QRCTSFKEKVRQQPHNVNAGLLTYPTLMAADILIHKATKVPVGKDQEQHLEMTRTFAN
                     RFNRLYKVDYFPEPQAFNFGEELIKVPGLNGSGKMGKSEGEDNAIFLADEPETIRRKV
                     MKAITDAGPTVPNQPKSEAVQNLFQLLNIVSSPETVNYFEEQYNTCQIKYGELKKQLA
                     EDMIAFTAPFRERIKEISNNDEYLRKVVRMGKEKAQAHASQTVKEVREIIGFRSF"
     gene            1207..1599
                     /locus_tag="PLR72_06735"
     CDS             1207..1599
                     /locus_tag="PLR72_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36930.1"
                     /translation="MKNFFSHGLLAIILLTGLVLGNGYLITENSKIAEISQHQGKEQN
                     SIPTQQYWFSITNFPLNIPANEEVRILPTSSFTFCRVLERLYFFKVRYSTLLVKEQYF
                     FLHKVSDYFFSYQALWGYYLFQLRKLLI"
     gene            1742..2272
                     /locus_tag="PLR72_06740"
     CDS             1742..2272
                     /locus_tag="PLR72_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36931.1"
                     /translation="MDTNLTIEESFDSLYKNEIGKSKQSPLVGTLLIVVGLILVLVSV
                     SIKSLEGTSMLYLLIMIGSIFAIVGIFVLLFRKTYYISVQTRKKLKPYEIYFDPKERD
                     KLVRIMQNKNLNELKSLKLAIQDGLKLRVFTTPDGEACFSQVVTFIPYEYVNVTPVMK
                     HTPEEAKILIETFKLK"
     gene            2728..3192
                     /gene="smpB"
                     /locus_tag="PLR72_06745"
     CDS             2728..3192
                     /gene="smpB"
                     /locus_tag="PLR72_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOK36932.1"
                     /translation="MHRPSNIIIKNKRANFEYEILDRYIAGIQLFGTEIKSIRDGKAS
                     LADTYCIFINHELWVKNMHIAAYSFGTYNNHEVRRDRKLLLTKRELLKLERANKESGT
                     TIVPLKLFINEKGLAKLEIALVRGKKLYDKRQSLKEKEDQRNIERALKQKEH"
BASE COUNT         1126 a          464 c          495 g         1129 t
ORIGIN      
        1 aagaataatt tagtttttta tctttaaatg attaattttg caaacgaaat tctataaaaa
       61 ataaaaaatt gatttaaata tttgattttc tcatcttata acaaattctc attttcagtt
      121 ttatcttgag aaaattaata aagagaatag gttgatatgg aaattgtact tagtggtatt
      181 cgtcctacag gaaaattaca tttaggtaat tattttggtg ctttaaggaa ttttgttcat
      241 cttcaaaatg aatacgattg ttattttttt attgctgatt atcattctct tacgactcat
      301 cctactccac acaatttgtt tggaaatgtt cgtgaagttt tagtagaata tttagcagcc
      361 ggtttagatc ccgaaaaagc aactttatat attcaaagcg atgttccaca agtgaccgaa
      421 ttgtatcttt tcttgaacat gaatgcttac atcggtgaat tacaacgctg tacttccttt
      481 aaagaaaaag ttcgtcaaca acctcataat gtaaatgctg gattgttaac ctatcctact
      541 ttaatggcag ccgatattct tattcataaa gctacaaaag tgccggtggg aaaagatcaa
      601 gaacagcatc tggaaatgac acgaactttt gccaaccgtt ttaaccgact gtacaaagtt
      661 gattattttc ctgagccaca agcatttaat tttggagaag aattaattaa agttccagga
      721 ttgaatggca gtggaaaaat gggaaaatca gaaggggaag acaatgccat ttttcttgca
      781 gatgaacctg aaacaattcg aagaaaagta atgaaagcta ttacggatgc tggtcctact
      841 gtaccaaatc agcctaaatc agaagctgtt caaaatcttt ttcaattgct taatatagtt
      901 tcctcacccg agacagtgaa ttattttgaa gaacaatata atacctgcca gataaaatat
      961 ggtgaattga agaaacaact ggctgaagat atgattgcct tcactgctcc ttttcgtgaa
     1021 cgaattaaag aaatatccaa taatgatgag tatttacgca aagtggtacg catgggaaaa
     1081 gaaaaggcac aagctcatgc ctcacaaacg gtgaaagaag tgcgtgaaat tattggtttt
     1141 agatcttttt aaataaaatt atacttttaa tgagaaattg agaaaaaatc taattgttat
     1201 aaaattatga aaaacttttt ctcacatgga ttattagcaa ttattctttt aacagggctc
     1261 gtcttgggga atggttatct catcactgaa aactcaaaaa ttgctgaaat aagccaacat
     1321 cagggaaaag agcaaaattc tattccaact caacaatatt ggttttccat cactaatttc
     1381 cctttaaata tccctgctaa tgaagaagtt agaattttac ctacttcttc atttacgttt
     1441 tgcagagttt tagaaagatt gtatttcttc aaagttcgtt actcgacttt attagtgaag
     1501 gagcaatatt tttttcttca taaagtttca gattattttt tttcttatca agcactatgg
     1561 ggatattatc ttttccaact tcgtaaatta ctaatttgat ttttccatct ttatttaatt
     1621 ctttcttaaa catttaatca attagatttc tctaattttt ttaaattatt tttattttaa
     1681 tttctctaaa aatcaattaa taagtatctg aaaaatactt acttaaaaga attatataat
     1741 catggataca aatttaacaa ttgaagaaag ttttgattct ctttataaaa atgagatcgg
     1801 aaaatctaag caatcacctt tggttggaac ccttttaata gttgtagggt taattttggt
     1861 attagtaagt gtttctatca aaagtttgga aggtacttcc atgctttatt tattgataat
     1921 gataggtagt atttttgcta ttgtgggaat ttttgttcta ctatttcgaa aaacttatta
     1981 tatttccgtt caaacacgta aaaaattaaa accttatgaa atttattttg atccgaaaga
     2041 aagagataaa ttagtgagaa tcatgcaaaa taaaaacttg aatgaactta aaagtttgaa
     2101 gttagctatc caagatgggc ttaaacttag ggtgttcact actcctgatg gtgaagcatg
     2161 tttttctcaa gtagttactt ttattcctta tgaatatgta aatgtaactc cagttatgaa
     2221 gcatacaccc gaagaagcaa aaatcctaat agaaacattt aagttgaaat agaaatttat
     2281 attaattttt tattttttaa aaaagggttg accaaataat caaccctttt ttcaacagtt
     2341 atattttatg gctgatttcc cctcattctt taaattcaga taaccattac tttatataat
     2401 ttgcaaagtg ctggtttaaa aagtctcgta ttttattaaa agggtttagt tttccttttt
     2461 taatccgttg atcaagctat tcaagtaaaa gataatattt tctttcgtgt acgattttaa
     2521 ccaagaatct cttctttgaa atttattctg acaataaatt gctttttgcc gaagaagatg
     2581 taactttgaa cattgttagt agatttttca cctaaacaat tggagaattt tttattaagc
     2641 aaaccttgaa gcctttttaa aactttccga tttttttatc atttctaact acaactttaa
     2701 tattttttca aaaatttaca tgaaattatg catcgacctt caaacataat aattaaaaac
     2761 aaacgtgcaa attttgaata tgaaatttta gatcgatata tcgctggtat tcagcttttt
     2821 ggtactgaaa ttaaatccat aagagatggt aaggcaagtt tggctgatac ctattgtatc
     2881 tttattaatc atgaattatg ggtaaagaac atgcacatcg ctgcttattc ttttggcact
     2941 tataataatc atgaagtccg ccgtgatcgt aaattattat taacgaagcg agaactgctc
     3001 aaactggaaa gagccaataa agaaagtgga acaactatcg taccgctgaa gctttttatc
     3061 aatgaaaagg ggttagctaa gttggaaatt gccttagtga ggggaaaaaa attgtacgac
     3121 aaacgtcaat ccctgaaaga aaaagaagat caaagaaata tagagagagc cttgaaacaa
     3181 aaagagcatt gaattcgctg cataaaaaat catt
//
LOCUS       DAOSAS010000156         3198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_19374_length_3198_cov_12.008591, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000156 DAOSAS010000000
VERSION     DAOSAS010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19374_length_3198_cov_12.008591"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1648
                     /locus_tag="PLR72_06750"
     CDS             239..1648
                     /locus_tag="PLR72_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36933.1"
                     /translation="MLNYFPKYFTQKAMLLYVISLAAVTGIFFSHAMSWVWIFFGLVE
                     VIGFFYFSNELTRHWSKTSPKVFTKRLFTNALIIRLVWVVFSYFFYKAMTGQPFEFDA
                     ADAAGYHNEASWLADMIHKGNIQPYFAYINGRFSDMGYPFYLGCLYTVTGKSILFARL
                     LKAFYGALTCVLVYKLTARNFEESTGRIAGILAMLMPNLIYYSGVHLKEAEMVFLTVA
                     FIERADALLRNRKFNFINIFVPTLLGASLFFFRTALGMTALFAVLTTLVFAPNKMVRS
                     WKRVIYGIWIGITIIYFAGTSISSEVESLWQNRKENQTISMQWRAERPGGNRFARYAS
                     STVFASLILVIPFPTMVNIEGQENQQTINGGNFVKNILAFFVIFALIWVIKNKVWRKY
                     LLIGSFTIGYLLILAFSGFAHSERFHQPALPFILILAAVGISRVTNRSKKYFWWWMVF
                     VFIAAIGWNWFKLAGRGLV"
     gene            1737..2666
                     /locus_tag="PLR72_06755"
     CDS             1737..2666
                     /locus_tag="PLR72_06755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK36934.1"
                     /translation="MKILIVCSGNAENFSLEKDQAFIYDQIKAIEYLYHDIQFEVFAL
                     KGKGIVGYMHEFEKLIDTIHHFQPDIIHAHGGHIGFLSIFQRKVPVVITFHGSDINKI
                     PNRFISFMAALFSDSCIFVSENLKKKLPFIKGQIIPCGVDLSIFYPINSTEAKKQLGM
                     NPDQKYILFPSSFTNRIKNFPLAQSVMHHFPNYQLMEISNRTRKEVNLLLNGADALLM
                     TSFSEGSPQIIKEALACNIRIVSVNVGDVREQLKGVDGCFCCQFNPEELTEKLKRSLA
                     GPRPTKGREKAENYSNTRIAEAIVVIYNQLLKK"
     gene            2663..2899
                     /locus_tag="PLR72_06760"
     CDS             2663..2899
                     /locus_tag="PLR72_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36935.1"
                     /translation="MKEVIIVDQIEGNVGSLFKAFKRLRISVKCSTDPIDLLHADHIV
                     IPGVGHFGKAMQRINDLNLIEPLEEAVVIKKYLF"
     gene            2908..>3198
                     /gene="hisH"
                     /locus_tag="PLR72_06765"
     CDS             2908..>3198
                     /gene="hisH"
                     /locus_tag="PLR72_06765"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOK36936.1"
                     /translation="MGMQLICRYSEEGNANGLGWIDAEVKRFNFNDILNYKSPHTGWN
                     TLYPKQKDHLLLKGIEPTEYFYFVHNYFVHCHNSENCLAETEYEQRFASVIQK"
BASE COUNT         1026 a          510 c          614 g         1048 t
ORIGIN      
        1 cttctgaatc ttgaaattgt cactgaaacg acgttgacga cgttcagccg ttgtcatttt
       61 taattcttct aatcgtgcca taataaatta atttagtaaa tttgttactt tattatagtc
      121 aacttatttc aagcattgac aagagatgcg ggatgcgagt taagaatgaa aagtaaggag
      181 taaggagtca agagagaaaa attagaatta ctcaaaactc agaactaaca agaaaagaat
      241 gctcaattat tttccgaaat attttactca gaaagccatg ctgctgtacg tcatttcttt
      301 ggctgcagta accgggattt tcttttcaca tgccatgtcg tgggtatgga tatttttcgg
      361 attggtggaa gtaattgggt ttttctattt ttccaacgaa ttaactcggc attggagtaa
      421 aacgtctcca aaagttttta ccaaacgact ttttaccaat gctttgataa taagactggt
      481 ttgggtggta ttcagctact ttttttataa agctatgacc ggtcaacctt ttgagtttga
      541 tgctgccgat gcggcaggtt atcacaacga agcttcttgg ttggctgata tgatccataa
      601 aggcaacatc caaccttatt ttgcttatat taatggacgc ttttccgata tgggatatcc
      661 cttttatctg ggttgcctgt ataccgttac aggaaaatcg attctctttg cacgactgct
      721 gaaagccttt tatggagcat tgacttgtgt tttagtatat aaacttaccg cacgcaattt
      781 tgaagaatca accggcagaa tagctggcat tctggccatg ttgatgccca atctgattta
      841 ttattctgga gttcatctaa aagaagccga aatggttttt ctgactgttg cttttattga
      901 acgagcagat gcattgcttc ggaaccgcaa atttaatttt atcaatatat ttgttccaac
      961 gcttttaggt gcttctctct tcttttttcg tacggcgtta gggatgactg ccttatttgc
     1021 agtgttaacc acattggttt ttgcacccaa taaaatggtt cgtagctgga aaagagttat
     1081 ttacgggatt tggattggta tcaccattat atattttgct ggcaccagca tatcttctga
     1141 agtggaaagt ctctggcaaa atcggaaaga aaatcaaaca atttccatgc aatggcgtgc
     1201 tgaacgtcct ggtggtaatc gttttgctcg ttatgcttcc agtacggtat ttgcctcgtt
     1261 aatacttgtc attcctttcc ctacaatggt gaatatcgaa ggacaagaaa accagcaaac
     1321 aataaatggt ggaaattttg taaaaaacat attggctttt tttgttatat ttgctttaat
     1381 atgggtaatt aaaaacaaag tgtggcgaaa atatttattg ataggttcat ttaccatcgg
     1441 ttatctattg atacttgctt tcagtgggtt cgcacattcg gaaaggtttc atcaaccagc
     1501 tttaccattc atattaatat tagcagcagt aggaattagt cgtgtgacaa accgttcaaa
     1561 aaaatatttc tggtggtgga tggtatttgt ttttatagca gctattgggt ggaactggtt
     1621 taaactggca ggaagagggt tggtataaaa agtttggagg atggagtgtg tggagataaa
     1681 tagaaaatta tcaaaactcc gaacgaatta ctaaaccgaa caaattatcg atctatatga
     1741 aaattttaat tgtttgtagc gggaatgcag aaaatttctc tttggaaaaa gatcaagcat
     1801 ttatttatga tcaaataaaa gccatagaat atttatatca cgacattcaa tttgaagttt
     1861 ttgcattaaa aggaaaaggg atagttggtt atatgcatga atttgagaaa ttgatagata
     1921 cgattcatca ttttcaaccc gatataattc acgcacatgg tggccatatt ggttttttaa
     1981 gtatatttca acgaaaagtt cctgttgtca ttacttttca tgggtcagat ataaataaaa
     2041 ttcccaacag gtttatctcc tttatggcag ctttattttc agattcatgc atttttgtat
     2101 cagaaaattt aaagaaaaaa ctacctttta taaaaggaca aattattcct tgtggagttg
     2161 atttatcaat tttttatcct atcaattcaa cagaagcaaa aaaacaattg ggaatgaacc
     2221 ctgatcaaaa atatattttg ttcccttctt ctttcactaa cagaataaaa aattttccac
     2281 ttgctcagtc cgttatgcac catttcccaa attaccaatt aatggaaatt tccaaccgaa
     2341 caagaaaaga agtaaatttg ttgctgaatg gggctgatgc tttattaatg acctctttct
     2401 cagaagggtc acctcaaatc atcaaggaag cattagcctg taacatacgg attgtttctg
     2461 taaatgtagg agatgtaagg gaacaactga aaggagttga tggatgtttt tgttgccaat
     2521 tcaacccgga agaactgact gaaaaactta aaaggtcact cgcaggccca cgtccaacaa
     2581 aaggaagaga aaaagctgaa aattacagca atacaagaat agctgaggct atagttgtta
     2641 tctataatca attgcttaaa aaatgaaaga agtaataatt gttgatcaaa tagagggaaa
     2701 tgtaggatca ttgtttaaag cttttaaacg tttaagaatc agcgttaaat gcagtaccga
     2761 tcccatagat ttattgcatg ctgaccatat tgttattccc ggagtaggtc attttggaaa
     2821 agcaatgcaa aggatcaacg atttaaatct tatcgaacca ttggaagaag ctgttgtcat
     2881 aaaaaaatac ctgttttagg tatttgcttg ggaatgcaat taatttgccg ttatagcgaa
     2941 gaaggaaatg cgaatggatt aggctggata gatgcagaag taaagcgttt taattttaat
     3001 gacatcctta actataaaag cccccatacg ggctggaata ctttgtatcc caaacaaaag
     3061 gatcatttac ttttaaaagg aatagaacca actgaatatt tttattttgt tcacaattat
     3121 tttgtacatt gtcataattc ggaaaattgt ttggcagaaa cggaatacga acaaagattt
     3181 gcctcagtca ttcagaaa
//
LOCUS       DAOSAS010000157         3163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_19669_length_3163_cov_9.351995, whole genome shotgun sequence.
ACCESSION   DAOSAS010000157 DAOSAS010000000
VERSION     DAOSAS010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19669_length_3163_cov_9.351995"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            471..2933
                     /locus_tag="PLR72_06770"
     CDS             471..2933
                     /locus_tag="PLR72_06770"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK36937.1"
                     /translation="MKKLIFLFFLNFFIGFTLSAQLLTTVKGQVVGPTENEPLPYVTI
                     SIAKDNNPEISIKKFATDIKGNFVISIPPGKYIFTFQFVGMNVLKKNVEVAGQTEIDL
                     GKIILEESSTQLNEINVVAQKPLVKVETDKITYNAKDDPESSTSNVLELLRKVPMVTV
                     DGEDKIQLKGSNNFKIYLNGKPSNMMTNNPSQVLKSMPASSVKNIEVITEPGAKYDAE
                     GVGGIINIVTDKRADDGYSGSIGANADIRGGYGGNGYLALKYGKVGFTGNASYYHFNQ
                     PDVESNYERHEFDSSLENLLKQDGTSKVNGGGLYLSGALSYEPDTMNLLNFSASRYGG
                     NYLVNTSQDALSQGIQNYSYHSKSSSTTEYGGINLSGDYQHNFKKKGEMLTVSYRYEE
                     NPNNSNFDNLFTDVSGNYFYPDGYRQQSINKATGKEHTGQVDYEIPFPQKHIFETGMK
                     YIFRKNYSTTDNSYYDVINEIWKTDTTQKNDLNHHQNILSGYVNYSFKTGKFGLNVGF
                     RGEYTFQRIHFMNSQDTIINTNFFDPVPSITVSYQLGMTKTLRAGYNMRISRPGIWYL
                     NPYVNNLNPTDIYYGNPELDAEHTHNFNINFGSFSQKTNLNTTLSYSLTNNAITSYSF
                     IKDGITHNTQANIGKNQNISLNIFGSWTPNQKLRLYANANINYTDIRSTNNTQLQNDG
                     FSGQIFSGLTLNLPKDFRLGANGGIFNSYIQLQTTQSSFYFYSFNLMKSLFNKKLDIN
                     LSTVNPFPESIAIKSQTKGVGFLQKTTYSEPIRNVRLSATFRFGDLKTTIKKVKKTIS
                     NDDIKAGGSDTQKGTGVIPAGN"
BASE COUNT         1167 a          472 c          495 g         1029 t
ORIGIN      
        1 gaaaaacatt tgattgttaa tatattttta gtgacaaaaa ctattctaca aaaattaaaa
       61 aattttagaa aaataacaag ataaattcaa gtaattaata ccatttataa gaagagcgaa
      121 aagtaaaatt attttttctt ttgcaagaac tttttactgg tactttgatg ctaatattct
      181 gtataataaa aaatcaatta aatattacta aaaatattat tttttttatt tggtaatgaa
      241 tctttattcc gaattttaca aatttaaaaa ttgtttttat gaatctacct ttaaaaacct
      301 gttttataaa ttttctaagg aaattcaata ttaatactca atgacttgca aatcactaaa
      361 ttattaaaat tgattttttt aagaggatca tgttatatta ttaatgaatc cgtagtttgg
      421 aatatttttc ttttatttaa atttaaataa atcctttgta aaaaacttta atgaaaaaat
      481 taattttctt atttttttta aatttcttta tcggttttac tttatcagct caattgctaa
      541 ctacagtaaa aggtcaagta gtaggtccaa ctgaaaatga gccgcttcct tatgtaacta
      601 tttctatagc aaaggataat aatccagaaa tatccattaa aaaatttgct actgatataa
      661 agggaaattt cgtgatttct attcctccag gtaaatatat ttttaccttt cagtttgttg
      721 gaatgaatgt tttaaaaaaa aatgtggaag ttgctggaca aaccgaaatt gatttaggaa
      781 agattattct tgaagaaagt tcaacacaac ttaatgagat aaatgttgtt gcccagaaac
      841 cgcttgtaaa agtagaaaca gacaagatta cttacaatgc caaagatgat cctgaatcct
      901 ctacttccaa tgttctggag cttttgagaa aagtaccaat ggtaactgtt gatggagaag
      961 ataaaatcca gttgaaaggt tccaacaatt tcaaaatcta tctgaatggt aagccttcta
     1021 atatgatgac taataatcct tcacaagtat taaaaagcat gcctgccagt agcgtaaaga
     1081 atatcgaagt tattaccgaa ccgggtgcaa aatatgatgc ggaaggagta ggtggaatta
     1141 ttaatattgt taccgataaa agggctgatg atggatattc tggatcaatt ggagcgaatg
     1201 ctgatattcg tggaggatat ggagggaatg gctatctggc attaaaatac ggaaaagttg
     1261 gattcacggg aaatgccagt tattatcatt tcaatcaacc tgatgtagaa tcaaactatg
     1321 agaggcacga atttgattct tcacttgaaa atttgctgaa acaagatgga acaagtaaag
     1381 taaatggtgg aggcttatat ttgagtggag ctttaagtta tgaaccagat acgatgaatc
     1441 ttttaaactt ttcagcatct cgatatggag gaaattatct tgtaaataca tcgcaagatg
     1501 ctttatctca gggcattcaa aattacagtt atcattccaa atcatcttca acaactgaat
     1561 atggtggaat taatttgtca ggcgattatc aacataattt caaaaagaaa ggagaaatgt
     1621 taactgtttc ttaccgttac gaagaaaatc ccaacaacag taattttgac aatctattta
     1681 cagatgtttc aggaaattat ttttatcctg atggttaccg ccagcaaagc attaacaaag
     1741 caacaggcaa ggaacataca ggacaagttg attatgaaat tccatttccc caaaaacaca
     1801 tttttgaaac cggaatgaaa tatattttca gaaaaaatta cagtaccacc gataattctt
     1861 attatgacgt tatcaacgag atttggaaaa cagatacgac tcaaaaaaat gatttgaatc
     1921 accatcagaa tattctatct ggctatgtaa attacagttt caaaactggg aaatttggtt
     1981 taaatgtcgg ttttcgcgga gaatatacct ttcaaagaat tcattttatg aattctcagg
     2041 acactattat caatacaaac ttttttgatc ccgtgccatc aattacagtt tcttatcagt
     2101 tgggaatgac aaaaacttta cgtgctggtt ataacatgcg tatttcacgc cctggtattt
     2161 ggtatctaaa tccttacgtt aacaatctta atccaaccga tatttattat ggaaatcctg
     2221 agttggacgc cgaacatacc cacaatttta atatcaattt tggatctttt tctcaaaaaa
     2281 ccaatttaaa cacaactttg agctattctt taaccaataa cgctataact tcttatagtt
     2341 ttataaaaga tggaataact cataatacac aagcaaatat cgggaaaaat caaaatatca
     2401 gtctgaacat ttttggaagt tggactccta atcaaaaatt acgtttgtat gccaatgcca
     2461 atataaatta tactgatatt aggagtacaa ataatactca attacaaaac gatggcttct
     2521 cggggcaaat ttttagcgga ttaacactaa atttaccgaa agattttcgt ttaggagcaa
     2581 atggaggcat ttttaatagt tatattcagt tgcaaaccac ccaatcctcc ttttattttt
     2641 attcatttaa cttgatgaaa agcttattta ataagaaatt agatattaat ctttctactg
     2701 ttaatccttt tcctgaatcg atagcaatca aatctcaaac taaaggggta ggtttcctgc
     2761 aaaaaactac ttattcagaa ccaattcgaa acgtgaggtt aagtgccact ttccgatttg
     2821 gtgatttaaa aaccaccatt aagaaagtca aaaaaaccat tagcaatgat gatataaaag
     2881 caggtggcag tgacactcaa aaaggtacag gggttattcc cgctggaaat taataatagc
     2941 aatagttgca ataaaactaa caattgaccg aaaaatttat gaataatact gccagaattg
     3001 aaacagaaaa ggaagaaact aattattgaa aattacgtga attttagaga gcaagtcgat
     3061 gacttttgaa aatttgtttg aaatacttta aattttttgc aatattgctg tctcactagg
     3121 attcgaacct agacaggcag atccagaatc tgacgtgcta cca
//
LOCUS       DAOSAS010000158         3159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_19704_length_3159_cov_13.555412, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000158 DAOSAS010000000
VERSION     DAOSAS010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19704_length_3159_cov_13.555412"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLR72_06775"
     CDS             complement(<1..344)
                     /locus_tag="PLR72_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36938.1"
                     /translation="MKQVLLILLGCLFILKIEANSYFQDKSHSLNYPQNVKVDVTSLN
                     WMKLSNDSSENESSLTGNPVSFSTTSEGIYLTLRNGKYDVQLFALTGQLLWSGNLVQG
                     RFFIPVRQGIYF"
     gene            complement(399..1973)
                     /gene="nadB"
                     /locus_tag="PLR72_06780"
     CDS             complement(399..1973)
                     /gene="nadB"
                     /locus_tag="PLR72_06780"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOK36939.1"
                     /translation="MTRKFDFLVIGGGIAGISFALKVAPYGKVALLCKTTLEESNTSL
                     AQGGMAAVFYPPDSFEKHIQDTLISGDGLCNIEAVKKVVYNAPEQIKQLIEWGVQFDK
                     TKDGKFDLHREGGHSEFRILHKKDYTGYEIQKKLNERVRNTPNIEIFEHHFAIDILTQ
                     HHLGEIVTHHTPNIECYGAYVLDETNDEIHTFLSKITLLATGGIGGAYLTSTNPPIAT
                     GDGIAMVHRARGVIKDMEFVQFHPTALYQPGVKPSFLISEAIRGYGAVLRTKDGNEFM
                     YKYDERKELAPRDIVARAIDNEMKLRGDEYVYLDVTHKDPKETKKQFPLIYKRCRELG
                     INITKDYIPVTPTQHYLCGGIAVDLNARSSINRLYAAGECSCTGLHGANRLASNSLIE
                     AIVYADAAAKDAIKQVNSLSFKEGIPDWNDEGTTYPEEMVLITQSVREVEQIMNTYVG
                     IVRSNLRLKRAMTRLEILFRETEDLFDRSVVSRQICELRNIISVSYLIIKQATFRKES
                     RGLHYNIDYPYHADTV"
     gene            complement(2113..>3159)
                     /locus_tag="PLR72_06785"
     CDS             complement(2113..>3159)
                     /locus_tag="PLR72_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36940.1"
                     /translation="NKYAAPPLFIDDYTNLTKGKNPSLDFCEAAYFLAHDERNKIVGR
                     IAMIINPVANETWNQKNARFGWIDFIDDYNVSKALFDTAVAWAKSKGMKAIHGPLGFT
                     DFDHEGTLIEGFDRLGTLATIYNYPYYPKHIERYGFVKDVDWLEFLITIPNKFPEKYF
                     RIAELVKEKYHLTPKQFKNRKYLVEKYGEKVFDLLNTCYEDLYGFTKLNDAQKKFYIK
                     LYFSFFRLDTVSIVTDENDEVVALGIAMPSLTKALQKAKGRLFPLGWFYLLRAIQKND
                     IVDLYLMAVHPRYQNKGVNSIIFAELMPKFAKNGYKFAESNPELETNVRVMNLWGNFE
                     YINHKRRRAYRLEI"
BASE COUNT         1000 a          580 c          513 g         1066 t
ORIGIN      
        1 aaaaaataaa ttccttgacg aactggaata aaaaatcgtc cttgaacaag attgccactc
       61 cataataatt gtcctgtcag agcaaacaac tgaacgtcgt actttccatt tctcaaagta
      121 agataaattc cttcagatgt agttgaaaaa cttacaggat ttcctgtgag ggaactttcg
      181 ttttcgctgg aatcattact taatttcatc caattgagag aagttacatc aactttcaca
      241 ttttgaggat aattcagtga atgagattta tcctgaaaat aggaatttgc ttctattttt
      301 agaataaata aacatcccaa taatattaac aacacttgtt tcattctgta atactattct
      361 ttttgctatt ttaataacaa aagaaagaga cgagttgttc acacggtatc ggcatgatat
      421 ggataatcaa tgttgtaatg caaaccgcga ctctcttttc ggaaagttgc ttgttttata
      481 ataagataag agacactaat aatatttctt agttcgcaaa tttgacgtga aacaactgag
      541 cggtcaaata aatcttctgt ttcacggaac aaaatttcca gccgagtcat agctctcttt
      601 aaacgtagat tggaacgaac aattcctaca taagtattca taatttgttc cacttctctg
      661 acactttgtg tgatcagcac catttcttca ggataagttg ttccctcgtc gttccagtcg
      721 ggaatacctt ctttaaaaga aagagaattc acttgtttta ttgcatcttt ggcagcggca
      781 tctgcatata caatagcttc aatcaatgag ttggaagcca gccgattggc tccatgtaaa
      841 ccggtacaag aacattcacc tgccgcatac agcctattga tggatgaacg tgcattcaaa
      901 tctacagcga ttcctccaca taaataatgt tgggtagggg ttactggaat gtaatctttc
      961 gtgatgttga tgcctaattc gcgacagcgt ttataaataa gcggaaactg cttttttgtt
     1021 tcttttggat ctttatgggt cacatctaaa taaacatatt catccccacg gagtttcatt
     1081 tcattgtcaa tagctcttgc tacaatatcg cgcggtgcta gttcttttct ttcgtcatat
     1141 ttatacataa attcatttcc atctttggta cgcaacacag caccataacc tcttatagct
     1201 tccgaaatca aaaaagaagg ttttacacct ggttgataaa gggcagttgg atgaaattga
     1261 acaaattcca tatctttgat tactccgcgg gcgcgatgta ccatagcaat tccatcacct
     1321 gtagcaatgg gtggattggt agatgtaaga taggcaccgc caattcctcc tgttgccagt
     1381 aaagtgattt tagataaaaa cgtatgaatt tcatcgttgg tttcgtccag cacataagca
     1441 ccataacatt caatattcgg agtatgatga gtcactattt cgcctaaatg atgttgtgta
     1501 agtatatcaa tggcaaaatg atgttcaaaa atttcaatat ttggagtatt tcgaacccgt
     1561 tcgtttaatt ttttttgaat ttcataacct gtataatctt ttttgtgaag gattctgaat
     1621 tctgaatgtc caccttctcg gtgaagatca aattttccat cttttgtttt atcgaattgt
     1681 acaccccatt caatcagttg tttgatttgt tcaggggcat tataaacaac ttttttcaca
     1741 gcttcaatat tgcagagtcc atctccagaa attaaagtat cttgaatatg tttttcaaaa
     1801 ctatcgggag gataaaaaac ggccgccatt cctccttggg caagcgacgt attagattct
     1861 tctaatgttg ttttacaaag caaggctact tttccataag gagcaacttt aagagcaaag
     1921 ctgattccgg caattcctcc accaattact aaaaaatcga atttacgtgt cataataatt
     1981 taattttaaa tcaaaagtac attataattg ggcttcacca cattcaaact ttttaaagtt
     2041 aaagtggaca tgtggagtct tcatcaaaat tttctgggtt gataaagaaa aaaatatttc
     2101 ataagaaaga ttttatattt caagtctgta agctcttctt cttttgtgat tgatatattc
     2161 aaaatttccc catagattca taacccgtac atttgtttct aattccggat tgctttcagc
     2221 aaatttataa ccatttttag caaatttcgg catcaattca gcaaatatta ttgaattgac
     2281 tcctttattt tgataacgag gatgtacagc cattaaatat aaatcaacaa tgtcgttttt
     2341 ttgaattgct cttaatagat agaaccagcc aagcgggaaa agtcttcctt ttgctttctg
     2401 aagagccttt gtcaaactgg gcatagctat tcccaatgct accacttcat cgttttcgtc
     2461 agtgacaata gaaaccgtat ctaatcggaa aaaactaaaa tagagtttga tatagaattt
     2521 cttttgtgca tcgtttaatt ttgtgaaacc atataaatct tcataacaag tgttgagtaa
     2581 gtcaaagact ttttctccgt atttttcaac taaatattta cgatttttaa attgtttggg
     2641 agtaagatga tatttttctt taacaagttc agcaattcta aagtattttt caggaaattt
     2701 attgggaata gtaattaaaa attccagcca atccacatct tttacaaagc catatcgttc
     2761 aatatgcttg ggataataag gataattata aatagttgcc aatgtgccta aacggtcaaa
     2821 accttcaatt aatgttcctt cgtgatcaaa atcagtaaaa ccaagaggtc catgaatagc
     2881 tttcatccct ttggattttg cccaggctac agcagtatca aacagagctt tggaaacatt
     2941 ataatcgtct ataaaatcta tccaaccaaa acgtgcattt ttctgattcc aggtctcatt
     3001 ggcaacaggg ttaataatca ttgcaattct acccactatt ttatttctct catcgtgagc
     3061 taaaaaataa gcagcttcgc aaaaatccag tgaaggattt ttccctttgg ttaaattggt
     3121 ataatcatct ataaacaaag gaggagcagc atacttgtt
//
LOCUS       DAOSAS010000159         3120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_20035_length_3120_cov_12.222838, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000159 DAOSAS010000000
VERSION     DAOSAS010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20035_length_3120_cov_12.222838"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLR72_06790"
     CDS             complement(<1..458)
                     /locus_tag="PLR72_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOK36941.1"
                     /translation="MTNPLYTPNPERYIHSVGYRFCGKSGLQLPAISLGLWHNFGDVD
                     DFQEAEKMIKFAFDHGMTHFDLANNYGYPPGSAEKNFGKILKENFSSHRDEMVISTKA
                     GHEMWAGPYGDGGSRKYMIASLHQSLKRMGLEYVDIFYSHRYDPNTPLEET"
     gene            complement(486..1067)
                     /locus_tag="PLR72_06795"
     CDS             complement(486..1067)
                     /locus_tag="PLR72_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOK36942.1"
                     /translation="MKKNVVIILIIVAALVLWGVSMYNSLVSSEEAVSSQWSNVENQY
                     QRRADLIPNLVATVKGYAAHERGTLEEVVQARAKATQITVDANSLTPEKLQQFQQAQG
                     ELSAALGRLLMIAENYPDLKANQNFIELQAQLEGTENRIAVERNRFNEMARNYNASIR
                     RFPKSIIAALFGFEKKPYFEAEAGTEKAPKVEF"
     gene            complement(1122..2435)
                     /locus_tag="PLR72_06800"
     CDS             complement(1122..2435)
                     /locus_tag="PLR72_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOK36943.1"
                     /translation="MKKLFVFFVVTCLIVMVSANEYTLKTIPKPKTLDQHNYVSNPDK
                     ILKDETVYAINNILDSLERKTGVEIAVVVVNSIGGEEIKSFATQLFNEWGVGKAKQDN
                     GLLILFVLDQQKVTFETGYGLEGILPDAICKRIQMQNMIPEFKKGNYDAGFLAAIKEI
                     ISIILHEPVPEKIVEPIHWNEILPIALIVYLVLISITWLWIESSIRKIRKNVKLKTNI
                     ARYVAIKNEKKGIISLMTVLPLIIGFISILIFLQPIFLLLLIPIPLTTLPAAIYANIK
                     MQKIRREPIPCNVCGGRMHILSEKDDDAYLKLSQRFEEQLHSVDYDVFICDNCKNEAI
                     FLLDKPSAYTQCPKCGTKSFILKEKKIIAAPTYLHPGTERITYHCLYCGYEENRNRRL
                     PRLTHSRNTIIAGSIPGGIFSGSGGFGGGSFGGGMSGGGGATSGW"
     gene            complement(2461..2907)
                     /locus_tag="PLR72_06805"
     CDS             complement(2461..2907)
                     /locus_tag="PLR72_06805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOK36944.1"
                     /translation="MKKIVFPFCLVALLFLFSCEEEKYADWKILNEQWLQSHKSDSGF
                     VSTPSGLCYKVIHQGYDRRPNPYSWVSVQYKGYLIDGTLFESGTYEYYLSNAIRGWIE
                     GITKMNGGGTYIFYIPSSLGYGKDGYGEIPPYSTLIYEVTLLSSAY"
BASE COUNT         1037 a          600 c          444 g         1039 t
ORIGIN      
        1 gtttcttcaa gaggcgtatt cggatcgtaa cgatgcgaat agaaaatatc aacgtattca
       61 agtcccattc gcttcagact ttgatgaaga cttgcaatca tatattttct ggagccaccg
      121 tcaccataag gacctgccca catttcatga ccagcttttg tactaataac catctcatcg
      181 cgatgggaag aaaaattttc tttcaagatt ttcccgaaat ttttctcagc actgccagga
      241 ggataaccat aattatttgc caaatcgaaa tgagtcattc catgatcaaa agcaaatttt
      301 atcatttttt cagcttcctg aaaatcgtcc acatccccaa aattgtgcca caaacccaaa
      361 gaaatcgcag gtaactgtag tccacttttt ccacaaaacc gataacctac tgaatgaata
      421 tatcgctcag gattgggagt ataaagagga tttgtcatag ttttgttttt tcttttaaat
      481 tgattttaaa attcaacttt gggggctttt tctgttccag cttctgcttc aaaataaggt
      541 tttttctcaa aaccaaataa agcagcaata attgattttg ggaaacgccg gatagaagca
      601 ttgtaatttc gagccatttc attaaaacga tttcgctcca ccgcaattct attttccgtt
      661 ccttccaact gtgcttgcaa ttcaatgaag ttctgattgg cttttaaatc tggataattc
      721 tcagcaatca tcaataatct acccaaagct gcacttaatt ctccttgtgc ttgttgaaac
      781 tgctgaagtt tttcaggcgt taatgaatta gcatctacag taatttgagt tgctttagca
      841 cgagcttgta ctacttcctc taatgttcct ctttcgtgag cagcataacc tttaactgtt
      901 gcaaccagat taggaattaa atctgctcga cgctggtact gattttccac attcgaccat
      961 tgacttgaaa cagcttcttc agaagagact aaactgttgt acatagaaac accccataag
     1021 acgagtgctg ctacaataat taaaataata acaacattct ttttcataaa ataatttttt
     1081 tatttgtttt tgatcaattt ctaattattt tttagccaaa tttaccagcc gctggtagca
     1141 ccaccgccac ctgacatacc tccgccgaaa cttcctccac caaaacctcc actaccacta
     1201 aaaattcctc cgggaataga acccgcaata atggtattac gtgaatgggt caaacgcgga
     1261 agtcggcgat ttcgattttc ttcgtaacca caataaagac aatgatatgt aatacgttct
     1321 gtaccaggat gtaaataagt gggtgcagct atgatttttt tctctttcaa aataaaagac
     1381 tttgttccac acttaggaca ctgagtatag gcacttggtt tatccagcaa aaaaatagct
     1441 tcatttttac aattatcaca tataaaaaca tcataatcta ctgaatgaag ttgttcttca
     1501 aaacgctgag ataattttaa ataggcatca tcatcttttt cggataaaat atgcatccga
     1561 ccaccacaca cattacaagg aatgggttct cttctgattt tttgcatttt tatatttgca
     1621 taaatagctg caggtaaagt tgttaaagga ataggtatca gtaataacaa aaaaattggt
     1681 tgtaaaaaaa taagaatact tataaaacct attataagag gtaacactgt cattaaggaa
     1741 atgattcctt tcttttcatt tttaatggca acataacgag caatgttagt ctttagtttt
     1801 acattctttc taattttacg aatggaactt tctatccaca accaagtaat tgatattaat
     1861 actaaataaa caattaaagc tattggtaaa atttcattcc aatgaatagg ttcaacaatt
     1921 ttttcaggaa caggttcatg aagaataata gaaattattt cctttattgc agctaaaaat
     1981 cccgcatcat aatttccttt tttgaattca ggaatcatat tttgcatctg aatgcgtttg
     2041 caaatagcat ctggtaaaat tccttccagc ccatatcccg tttcgaaagt aactttttgt
     2101 tggtccaaaa caaataatat caataagcca ttgtcctgtt ttgcttttcc aaccccccat
     2161 tcattaaaaa gctgagtagc aaatgatttt atttcttcgc ccccaattga atttacaact
     2221 actaccgcaa tttcaactcc tgtttttctt tcaagtgaat ccaaaatatt attaatggca
     2281 taaacagttt cgtctttcag aattttgtca ggattactca catagttgtg ttgatccagt
     2341 gtttttggct taggaattgt ttttaaagta tattcgttag ccgaaaccat tactattaaa
     2401 caggtaacaa caaaaaagac aaacaatttt ttcattatta aacgaaattt tcatacaaaa
     2461 ttaatacgca gatgaaagaa gtgttacttc ataaattaat gtggaataag gaggaatctc
     2521 tccataacca tcttttccat aacctaatga actagggata taaaaaatat aagttcctcc
     2581 tccattcatt ttggtaattc cttctatcca ccctcgaatg gcattcgaca aataatattc
     2641 atacgtacca ctttcaaata aagtaccatc aatcaaataa cctttatatt gcacagatac
     2701 ccaactataa ggatttgggc gacgatcgta tccctggtga atgactttat aacaaagtcc
     2761 gcttggggta gaaacaaagc ccgaatcgga tttgtgactc tgcaaccatt gttcatttaa
     2821 aattttccag tcagcatatt tttcttcttc acaagaaaaa agaaaaagaa gagctaccaa
     2881 acaaaaagga aaaacaattt ttttcatatc attttttatt cttaaaatac tcaaatttct
     2941 agatttttgt gtaacttaga atctagtaaa tcaacgtatt tgattttaca gcaacatcaa
     3001 aacaaaagat gttaaattga atttcttcca aagtttttta tctaaatata ggaatatcct
     3061 tgagaatttt ccggctcttt tatacttatt tatcaaaaaa atcaaaaagt aatttacttt
//
LOCUS       DAOSAS010000160         3109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_20134_length_3109_cov_10.617878, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000160 DAOSAS010000000
VERSION     DAOSAS010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20134_length_3109_cov_10.617878"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1187)
                     /locus_tag="PLR72_06810"
     CDS             complement(126..1187)
                     /locus_tag="PLR72_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOK36945.1"
                     /translation="MKKHLLFLLLLIIFLFFGFSSKNSVAFSSDKVNVQVACVAFYNL
                     ENLFDTIDNENVNDEEFLPNGPYRWNSMKYYSKLEHLSYAISQIGLDVTPVGAIIVGV
                     SEVENRGVLEDLVKQPTLINRSYEIVHYDSPDRRGIDVALLYNPRYFIVTNSKSYRLH
                     TQDTTFRTRDQLMVSGYLFDEKIHVIVNHWPSRSGGELASRPKRNAAAALTRSIVDSL
                     YRVDPKAKIIIMGDLNDDPFNESCAKILGAKKNPKDVKDGDLYNTLWKTFDKGIGSLA
                     YNDQWNLFDQLIISAPLLHAPASELRFWRAEVFNRDFLIQKEGRYKGTPLRTHAGGVW
                     LNGYSDHFPVLLYLIKQIQ"
     gene            1601..>3109
                     /locus_tag="PLR72_06815"
     CDS             1601..>3109
                     /locus_tag="PLR72_06815"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK36946.1"
                     /translation="MRKLVVVLFIVMHSFTILIAQTSLKGVVKIAETDNPLPGVKVTL
                     LKQNISTVTNPNGEFTLSYLEAGDEEIRFTKDGYFSQIKFIHLKADAENDLGVIYLRE
                     DLAQQMKQDIILQLSESQLENEEGRATQNISPVLASKGDVYLSQASYSFSPMRFSFRG
                     YDQNYESTYINGVYFNDIERGLFNYSSLGGLNDAMRNQDKTYGISPATFSYGNLGGVT
                     NIITKASTYATGTKANVAYSNRSYKLRGQATYATGLMQNGWAFAGSAVVRWADEGIIE
                     GTFYNSAGFFFSAEKVFNKQHSLSFVAFGAPTQRAQQGATTQETFDLVGSIYYNPYWG
                     YQEGKKRNSRIVKSFDPAAILSHEFKIDEKKLLKTGIAFHYNFYSNSALAYYNAPDPR
                     PDYYRYMPSFQQNEDVRNQMISLWKTDPNVSQVNWEELYLANYRNNIVNPNGTAKYAV
                     ERRHNNAMEIALNSTYNQQFTKKLKLTGGIEGKMNKGMHYKTMDDLLGGNQWI"
BASE COUNT         1103 a          511 c          490 g         1005 t
ORIGIN      
        1 gccaaaaatt taaaacgata gcaaaattac aaaaaatatc ccttatgaat aatcatcaca
       61 attttatttc tatcaaaaca ttttttgtta gacagataaa tttcctatat tgatgaattg
      121 taaaattatt gtatttgctt aatcaaatag agtagaacag ggaaatggtc gctataacca
      181 tttaaccaca cgccaccagc atgagtacgc aagggagtac ctttataacg tccttctttt
      241 tgaattaaaa aatcacgatt aaatacttct gctctccaaa atctcaattc actggcagga
      301 gcatgaagta aaggagcaga aataataagc tgatcaaata aattccattg atcattatat
      361 gccagcgaac ctattccttt atcaaaagtt ttccataaag tattatataa atcgccatcc
      421 tttacatctt tggggttctt ttttgcacct aaaatttttg cacaactttc attgaaagga
      481 tcatcgttga gatcacccat aataataatt tttgcttttg gatccactct atatagagaa
      541 tcaacaatag aacgggtaag agctgccgca gcatttcgtt taggtcgtga agctaattca
      601 cctcctgaac gagaaggcca gtgattgaca atcacatgaa ttttttcatc aaacaaataa
      661 ccacttacca ttagttgatc tcgggtgcga aaagtggtgt cttgtgtatg taatcgatat
      721 gatttgctat tggttacaat aaaatatctg gggttatata atagcgccac atcaatgcca
      781 cgtcggtctg gactgtcata atgaacaatt tcataagaac gatttattaa agttggttgt
      841 ttaaccaaat cttctaacac acctctattc tcaacctctg atacaccaac tataatggct
      901 ccaactggtg tcacatccaa tccaatttga gaaatagcat acgaaagatg ttcaagtttt
      961 gaataatatt tcatagaatt ccaacgataa ggaccattgg gtaaaaattc ttcatcattt
     1021 acattttcat tgtcaatcgt gtcaaataaa ttttccagat tataaaaagc aacgcatgca
     1081 acttgaacat taactttatc agacgaaaat gctactgagt ttttagaaga aaagccaaaa
     1141 aataggaaaa tgattaaaag cagcagaaat aacaaatgtt ttttcatata tgaaaatgtt
     1201 taatttattt tcttaaaaga acaacaaaag tgtagaaaat atttgaaaga aaaaacaatt
     1261 ttcggaggaa aattcaattt taatcattcc ctaaaatatt tttgcttttt tatcattaat
     1321 ccaattcgct gatttaaagc actcaacaaa atatttttag taaaatgtta aatttttatt
     1381 ataatttttc attatatgag cttttcttct ggttttcaat tagaatttta taaattgctt
     1441 gccatattta gttgtaaaaa atagaaaaac aaaacctgtt gaaaaaattt tatctgaaaa
     1501 tttattcaat aattcaaaaa aaagttttac cttgtcaccc ctttttaaaa attcatacag
     1561 aatattttaa ataaatttta cataaattaa ttttttactt atgagaaaat tagtagtggt
     1621 tttatttatc gttatgcact cttttacaat tttaattgca caaacctcat taaaaggagt
     1681 agttaaaata gcagaaactg ataatccttt gcctggcgtt aaagtaactt tattaaaaca
     1741 aaacatttct acggttacta accctaatgg agaatttact ttaagttatt tagaagcagg
     1801 cgatgaagaa attcgtttta ccaaagatgg ttatttcagt caaattaaat tcattcattt
     1861 aaaagcggat gcagaaaacg atcttggtgt catttatttg cgtgaagacc ttgcacagca
     1921 aatgaaacag gatattatcc ttcaattaag cgaaagtcaa ttggaaaatg aagaaggacg
     1981 agctactcaa aatatttcac ctgtattggc aagtaaaggc gatgtgtatc tttctcaggc
     2041 aagttattcc ttcagtccca tgcgttttag tttccggggt tacgatcaaa attatgagtc
     2101 aacttatata aatggtgttt attttaacga tatcgaaaga ggtttgttta attattcctc
     2161 tttaggaggc ttaaatgatg ccatgcgaaa tcaagataaa acttatggaa ttagtccagc
     2221 tacttttagt tatggcaatc ttggaggcgt taccaacatt attacaaaag ccagtactta
     2281 tgcaaccggc acaaaagcaa atgttgccta cagcaatcgt tcttacaaac ttagaggaca
     2341 agctacttat gcaacaggat tgatgcaaaa cgggtgggct tttgctggct cagcagtagt
     2401 acgttgggca gatgaaggaa ttattgaagg aacattttat aattctgctg gatttttctt
     2461 ttctgccgaa aaagtcttca ataagcaaca tagtttatca tttgtggctt ttggagctcc
     2521 tactcaacga gctcaacaag gtgctactac acaagaaacc tttgatttgg taggctccat
     2581 ttactataat ccttattggg gatatcaaga agggaaaaaa cgtaattcaa gaatcgtgaa
     2641 aagttttgat ccggctgcta ttctttctca tgaatttaaa attgacgaaa aaaaattatt
     2701 aaaaaccggc atagccttcc attataattt ttatagtaat tctgcccttg cttattataa
     2761 tgctccagat cctcgtcctg actattaccg ttatatgcct agttttcaac aaaatgaaga
     2821 tgttagaaat caaatgatct ctctgtggaa aaccgatcca aatgtttcac aagtcaattg
     2881 ggaagaactt tatttggcaa actaccgaaa taacattgta aatcccaacg gcacagcaaa
     2941 atatgctgta gagcggcgtc ataataatgc aatggaaata gccttgaatt ctacttataa
     3001 tcaacagttt acaaaaaaac ttaaacttac tggcggtatt gaaggaaaaa tgaataaagg
     3061 tatgcactat aaaacgatgg acgacctgtt aggtggaaat caatggatt
//
LOCUS       DAOSAS010000161         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_20184_length_3103_cov_14.826444, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000161 DAOSAS010000000
VERSION     DAOSAS010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20184_length_3103_cov_14.826444"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(629..2086)
                     /gene="pyk"
                     /locus_tag="PLR72_06820"
     CDS             complement(629..2086)
                     /gene="pyk"
                     /locus_tag="PLR72_06820"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOK36947.1"
                     /translation="MMTKFTKIIATISDKKCDVDFIRQLYNEGMNVVRMNSAHLNRDG
                     FLKIINNVRSVSRDIALLIDTKGPEVRTTITKNETIDLHRGDIIKITGNPQLISNKEC
                     IAVSYPFFVRDLNVGDDILLDDGEIELKVEDKDEQYLYCKVLNDGILGSRKSVNVPGV
                     RMNLPSLTERDRENILFAIENDLDFIAHSFVRNKQDILDIQQILDEHNSPIKIISKIE
                     NQEGVDNIEEILEYTYGVMIARGDLGIEVPQEKIPGIQRLLIRKCVMAKKPVIVATQM
                     LHSMINNPRPTRAEVTDVANAIYYRTDALMLSGETANGKYPVEAVRTMSRIIEEAEKT
                     KLIENDIPIPMTNLDVTSFLANAAVEACSKLDIKAIITDVYTGRTARNLAAYRGTKPV
                     YAICYYERLTRVLALSYGIFPHYQKETSSSQEYLIQALKDLLDKKLLNEEDNICYLSG
                     SLGEGHGTTFLEINQIKKIFEAGNNYSLLNFLKEI"
     gene            complement(2119..2541)
                     /gene="aroQ"
                     /locus_tag="PLR72_06825"
     CDS             complement(2119..2541)
                     /gene="aroQ"
                     /locus_tag="PLR72_06825"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOK36948.1"
                     /translation="MKRIQIINGPNLNLLGVREPTIYGNMSFETYIQKLKAQFTDIEL
                     EFYQSNHEGDIIDKLQEVDSSWDGIILNAGAYTHTSIAIADAIRSITTPVIEVHISNV
                     FQRESFRHQSFLSPVCKGCIIGFGLDSYRLAIEAILKL"
     gene            complement(2538..2819)
                     /locus_tag="PLR72_06830"
     CDS             complement(2538..2819)
                     /locus_tag="PLR72_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HOK36949.1"
                     /translation="MVFNILVIGYAAGFCTAIAQFPQAYKVFKTRDTHSISLAMYLIM
                     TFGVFLWFIYGLLLPDLPMILANGIGLIPSTFTLIITFQNVKRSKKQSV"
BASE COUNT         1098 a          522 c          448 g         1035 t
ORIGIN      
        1 ctaaaaaagt tacaaaaata tctttacctt acttattaac agagaattgt ttttagagat
       61 ttctcattta tttaatagaa aaattacctt attttgctta gcctcttggc tacgtctggg
      121 aaaaaataaa atcgggagat tttattttag cttttaataa agtaataatg ttattagtgg
      181 agaaaaaaat tcagagttat gaaagtcaat aatttatgat gaggtttttt aaatacaaaa
      241 ccataaacta tttaatgatg gaatgcgaaa aataccgatc tttatagaga cgctatatat
      301 gatgtctctc tgaatatgtt gaataacagt tgattattca taataaagga gtcttgtaat
      361 tttttaatac aataaaacaa ggtaattttt ctaccttatt tttctgaaaa ccttcataac
      421 aagggataaa aacccataaa atcaaacctt gttagcttat aaaaaaatta aacacttttt
      481 aatgcaaaaa aattagggaa gttgggtgca aaatccctat taaaaatcaa tattttatcg
      541 tacctcagat attgtgaatg tcctgttatt ttaatttaaa ttttattcct gaataacact
      601 tgttatcaga actttatatc aaaaaacact aaatttcttt aaggaaatta agtaatgaat
      661 aattatttcc cgcctcaaaa attttcttta tctgattgat ctctaaaaag gtagttccat
      721 gcccttctcc taaactacca cttaagtaac aaatattgtc ttcttcattc aataattttt
      781 tatccaataa gtcctttaac gcttgaatta aatattcctg gctgctactt gtttcttttt
      841 gatagtgagg gaaaatacca tatgataaag ctaatacacg tgttaatctt tcataataac
      901 aaatggcata aacaggcttg gtaccacgat aagctgctaa atttcgagcc gtacgcccag
      961 tataaacatc agtaataatt gcctttatat caagtttaga acaagcttca actgctgcat
     1021 ttgctaagaa agaagtaaca tctaaatttg tcataggaat aggaatatca ttttcaataa
     1081 gttttgtttt ttcagcttct tcaatgatac gtgacatcgt gcgtacagct tcaacagggt
     1141 attttccatt agcagtttca ccacttaaca taagggcgtc tgtacggtag taaatagcat
     1201 ttgctacgtc tgttacttca gctcgcgtag gtctgggatt attgatcatg ctgtgaagca
     1261 tttgggtggc aacaatcact ggcttttttg ccattacaca ttttctaatc aacagtctct
     1321 gaattccagg aattttttct tgtggaactt caattcctaa atcaccccga gcaatcatca
     1381 caccataggt atattccagt atttcttcaa tattatcaac accttcttga ttttcaatct
     1441 tagaaattat tttgattggg ctgttatgtt catccaaaat ttgctgaata tcaagaatat
     1501 cttgtttatt acgaacaaaa gaatgggcaa taaaatccag atcattttca atagcaaaaa
     1561 gaatattttc acggtctcgc tctgtgagtg aaggtaaatt cattcgaacg ccaggaacat
     1621 ttacactttt acggctacct aaaattccat cgttcaaaac cttgcaatat aaatattgct
     1681 catctttgtc ctcaactttc aattcaattt ctccatcatc taaaagaatg tcatcgccaa
     1741 cgttcaggtc acgaacaaaa aaaggataac ttacagcaat acattcttta tttgagatca
     1801 attgcggatt gcctgtaatt tttataatat cgccacgatg taagtcaatt gtttcgtttt
     1861 tagttatagt agtgcgaacc tcaggacctt ttgtatctat caacaaagca atatccctac
     1921 tcacagagcg aacattattt ataattttca aaaatccgtc gcgatttaag tgagctgaat
     1981 tcatcctaac aacgttcatt ccttcgttat aaagttggcg gataaaatcc acatcgcact
     2041 ttttatcact gatagtagct ataatttttg tgaatttcgt cataatattc aactattaaa
     2101 tgctctataa ataacacctt atagctttaa gattgcttca attgccagac gataggaatc
     2161 caatccgaaa ccaatgatac atcctttaca aaccggtgag agaaaagatt gatgacgaaa
     2221 actttctcgc tgaaaaacat tggaaatgtg tacttcgatc acaggagtag taatagaacg
     2281 aatagcatcg gcaatagcaa tagaagtatg tgtgtaagca ccagcattta aaataatgcc
     2341 atcccaagaa gaatcaactt cctgaagctt gtcaataata tctccttcgt gattcgactg
     2401 ataaaactcc agctcaatgt ccgtgaattg agcttttaat ttctgaatat aagtttcaaa
     2461 gctcatgttt ccgtaaatag ttggttctcg cacacccagc aaattgagat taggaccatt
     2521 aataatttga atacgtttca tactgattgt tttttagaac gtttcacatt ttgaaaagta
     2581 ataatgagag taaaagtaga aggaatcaat cctatgccat tggccaagat catgggaaga
     2641 tcaggcaaca gtagcccata aataaaccac agaaaaacac caaacgtcat tatcagatac
     2701 atagcaagag aaatactatg ggtatctctt gttttgaaaa ctttgtaagc ttgtggaaat
     2761 tgtgcaatag ctgtacaaaa gcctgccgca tatcctataa caagaatatt aaaaaccatg
     2821 aggatttatt ttattttatg caaaattaag aaaaaagtta ggttgataaa attctaaata
     2881 ttactaacaa gagcaatgca taaaaaattt caaaaatgag tagttaatta ataatttatt
     2941 aattgattat taataaatta tattataaaa tcacaaaaaa tttgtctatc tttaaagcaa
     3001 aaaacatctt tacacaagaa ttttgagaat tttcatgatt attgtgtcaa cagaaacaaa
     3061 aacataaact atcgaaattt tatccattct tatagctgtt tac
//
LOCUS       DAOSAS010000162         3092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_20291_length_3092_cov_14.217320, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000162 DAOSAS010000000
VERSION     DAOSAS010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20291_length_3092_cov_14.217320"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /gene="argB"
                     /locus_tag="PLR72_06835"
     CDS             complement(<1..731)
                     /gene="argB"
                     /locus_tag="PLR72_06835"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOK36950.1"
                     /translation="MKEELFIIKVGGKIIEDEVALQQLLIDFSKLEGNKVLVHGGGQL
                     ATTLATKLGIPTKMVDGRRITDDSMLKIITMVYAGWVNKQIVAKLQALDVNALGLTGA
                     DLNFISSEKRPIGEIDYGLVGDVKKVDATLISNLIEKKVVPVVAPVTHDKAGNLLNTN
                     ADTIAAEVAKAFAEWFEVTLIFCFEKKGVLLNEEDEESVIAELNSSLFEQYVAEKVIQ
                     GGMIPKLQNSFDALKAGVKEVWITRA"
     gene            complement(773..1297)
                     /locus_tag="PLR72_06840"
     CDS             complement(773..1297)
                     /locus_tag="PLR72_06840"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOK36951.1"
                     /translation="MDLEQVIASIRNIPDFPKKGVQFKDITTAMKDADVLQFVSDELY
                     DYYKDKGITKVVGLESRGFVLGSILAYKLHAGFILIRKAGKLPAATYKVEYELEYGTD
                     SLEIHQDAMNENDIVLLHDDLLATGGSSSAALELIKKFHPKAIYVSFLIELKDLNGRE
                     KLKGVNDIHSLIQF"
     gene            complement(1377..2333)
                     /locus_tag="PLR72_06845"
     CDS             complement(1377..2333)
                     /locus_tag="PLR72_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HOK36952.1"
                     /translation="MKKLMYVKDIGNLQEALQEAFEVKKNRFGFQELGKNKTLLMVFF
                     NSSLRTRLSTQKAGMNLGMNTIVLDINQGAWKLETERGVVMDGDKSEHILEAIPVMGC
                     YCDVIGVRAFAQFENKEYDYQETLINQFIQYSGRPVFSMEGATTHPLQAFADLITIEE
                     YKKRPHPKVVLTWAPHPKPLPQAVANSFADFMNEADVEFVITHPKGYELAPQFVRDAR
                     IEYNQMKAFEGADFIYAKNWSAYQDPNYGKILSKDLSWTVSSRQMAVTNNAYFMHCLP
                     VRRNMIVTDEVIESPQSLVIPEAANREISAQVVIKRMLNSLS"
     gene            complement(2361..>3092)
                     /locus_tag="PLR72_06850"
     CDS             complement(2361..>3092)
                     /locus_tag="PLR72_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOK36953.1"
                     /translation="GFGKTGKAVASVILQSPKFDLQWVLRRKKFLDDRNAAEILGVES
                     DNQGLIHSIYSINIDELLDKQPVDAIIDFSSEEGIYIYGEAAAKRKIKIISAISHYKK
                     ETKDFLKSLSKKTTVFWSPNITLGVNYLLICAKFLKKIAPWVDIQIIEEHFKGKKNVS
                     GTAITIAEALDINKTEINSVRAGGIVGKHEVIFGFPYQTVRLIHESISREAFGNGVLF
                     VAENLENKETGLYNFEELLLPYFTA"
BASE COUNT          964 a          610 c          439 g         1079 t
ORIGIN      
        1 gcacgtgtaa tccaaacttc tttaacacca gctttgagag catcaaatga attctgtaat
       61 ttgggtatca ttccgccttg aatcactttt tcagctacat attgttcaaa aagtgaagag
      121 tttaattcag caatgacact ttcttcatct tcttcattca acaaaactcc ttttttctca
      181 aagcaaaaaa ttaaagttac ttcaaaccat tcagcaaagg cttttgcaac ttcagcagca
      241 atggtatcag cattggtatt tagtaaatta cctgctttgt catgagttac aggagctacc
      301 actggaacga cttttttttc aatcaagttg ctgattagcg tcgcatcaac tttttttacg
      361 tcgcccacta aaccataatc aatttctcca atgggacgtt tttctgaaga gataaaattc
      421 aaatctgcac ctgttaaacc caaagcattc acatcaagag cctgtaattt agcaacgatt
      481 tgtttgttta cccagccagc ataaaccatt gttatgattt tcagcatgga atcatcagtg
      541 attcttctgc catcgaccat ttttgttggt attccaagtt tagtagccaa tgtagttgcc
      601 aattgcccac caccatgaac caagacttta ttgccctcca gttttgaaaa atcaatcaac
      661 aattgctgta acgcaacttc atcttcaata attttgccac ctacttttat tatgaatagc
      721 tcttctttca cttttattac ctttaagaaa tgagttgaaa aattttattg gcttaaaatt
      781 gaataaggga atgaatatca tttacacctt tcaatttttc cctgccgttt aagtctttca
      841 attcaatcaa aaaacttaca tagattgcct taggatgaaa tttttttatt aattctaacg
      901 ctgcactgga acttccacct gtagctaaca aatcatcatg taaaagtaca atatcatttt
      961 cattcattgc atcttgatga atttcaagtg aatctgtgcc atattccagt tcgtattcca
     1021 ctttataagt tgctgctgga agttttccgg cttttctaat caatatgaac cccgcatgaa
     1081 gtttataagc cagaatgctt cccaaaacaa aaccacgact ttccaaaccg actactttcg
     1141 taataccttt gtctttgtaa taatcataaa gttcgtcaga aacaaactgt aaaacatccg
     1201 catctttcat tgccgtggta atatctttaa attgcactcc ttttttggga aaatcaggga
     1261 tgtttctgat gctggcaata acttgttcca aatccatatt ttttttaact taatgtttta
     1321 atttttagat aaatatatat ttaaccttgt tataaaagtg taaaaataat tcttgctcaa
     1381 cttaatgaat tcaacattct tttaataacc acttgagccg aaatttctcg gtttgcagct
     1441 tcaggaatca ccagtgattg tggactttca attacttcat cggtcacaat catatttctt
     1501 ctgacgggta aacaatgcat aaaataagca ttattagtta ctgccatttg acgactactt
     1561 actgtccagc ttaaatcttt ggacaaaatt ttaccataat taggatcttg ataagctgac
     1621 caattcttag cataaataaa gtctgctcct tcaaaagctt tcatctggtt atattctatt
     1681 ctggcatcac gaacaaattg aggagccagc tcataacctt tgggatgcgt aatcacaaat
     1741 tctacatccg cttcattcat aaaatcagca aaagaatttg caactgcttg tggaagcggc
     1801 tttggatgag gtgcccacgt aagaactact tttggatgag gtcttttttt atattcttca
     1861 atggtaatta aatcggcgaa agcttgcaaa gggtgtgtag ttgcaccctc catgctgaaa
     1921 acaggacgac cagaatattg aataaactga ttgattaacg tttcttgata atcatattct
     1981 ttgttttcaa attgagcaaa agcacgtaca ccaatcacgt cgcagtaaca acccattaca
     2041 ggaatcgctt ctaaaatatg ttccgattta tcaccatcca tcacaacacc acgttctgtt
     2101 tcaagtttcc atgcaccttg attgatatcg agcactatgg tgttcattcc caagttcatc
     2161 cctgcttttt gagtactcag tcgagttcgc agactggaat taaaaaaaac catcagcaat
     2221 gttttgtttt tacctaattc ttgaaatcca aaacgatttt ttttcacttc aaaggcttcc
     2281 tggagagctt cttgcaaatt tccgatatct ttaacataca taagtttttt cattgacaca
     2341 ttatttcaaa taaatgaatt ttatgcggta aaataaggaa gaagtaattc ttcaaaatta
     2401 tataaacctg tttctttatt ttccaagttt tctgcaacaa ataaaacccc atttccgaaa
     2461 gcttctctcg aaattgattc atgtattaat cgaacagtct gataaggaaa tccaaaaata
     2521 acttcatgtt taccaacgat gccacctgca cgaacagaat taatttctgt tttattgata
     2581 tctaatgctt ctgctattgt tattgcggtt cctgatacat ttttttttcc tttaaaatgt
     2641 tcttcaataa tttgaatatc tacccacgga gcaattttct ttaaaaattt agcacaaatt
     2701 aataaataat taactcctaa tgtgatattg ggtgaccaaa aaacagtcgt cttttttgat
     2761 agagatttta aaaaatcttt ggtttctttt ttataatgag aaatagccga gataattttt
     2821 atttttcttt ttgcagccgc ttctccataa atataaattc cttcttctga agaaaaatca
     2881 ataatagcat caacaggttg tttgtcaaga agttcgtcaa tattaatgct gtaaatagaa
     2941 tgtatcaaac cttgattgtc tgattcaacg cccaaaattt cggcagcgtt tctgtcatct
     3001 aaaaattttt ttcttcgtaa aacccattgt aagtcaaatt ttgggctttg taaaataaca
     3061 gaagcgactg ctttccctgt tttcccaaaa cc
//
LOCUS       DAOSAS010000163         3049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_20685_length_3049_cov_14.097528, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000163 DAOSAS010000000
VERSION     DAOSAS010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20685_length_3049_cov_14.097528"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..953
                     /gene="mdh"
                     /locus_tag="PLR72_06855"
     CDS             12..953
                     /gene="mdh"
                     /locus_tag="PLR72_06855"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOK36954.1"
                     /translation="MSKVTVVGAGNVGATCVNVLAYNHIVDEVVMLDVKNGIAEGKAL
                     DMIQTATLLHFNTRITGCTNDYAQTANSDVVVITSGVPRKPGMTREELVEVNAGIVKE
                     VAEKILKFSPNAILVVVSNPMDTMTYLTYKATGLPRNRVIGMGGTLDTARFKHYLSIA
                     LDANPNEIEALVIGAHGDTSMIPLVRYASYKCRPVTELLDKDTLNKVVSDTMLGGSTI
                     TNLLGTSAWYAPGAAAAFLVEAIIHDERKIIPCSVYLEGEYGQNDICLGIPALIGKNG
                     IEKILQYSLNYEERELFRKSAASVHETNDTLKKLVEI"
     gene            1152..1571
                     /locus_tag="PLR72_06860"
     CDS             1152..1571
                     /locus_tag="PLR72_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOK36955.1"
                     /translation="MIYHFNSYQYLIKHSIRPSVQRTAIMDYLINNRNHPTVDEIYLA
                     LVPGMPTLSKTTVYNTLNLFVERGAVQVLNIDEKNARFDINTSIHAHFICSRCGKIQD
                     IFEISPENLEIKENHDFVVAKVEISYHGICKDCLSSN"
     gene            1770..2780
                     /locus_tag="PLR72_06865"
     CDS             1770..2780
                     /locus_tag="PLR72_06865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK36956.1"
                     /translation="MPDFTPGETNKNILSVSVIVPCHNEEKNLLSLFEALSVQTYQDF
                     ELLLINDHSTDATESIIKNHLPIFKNAVYFSSKEKGKKKALQEAIELAKGELIITTDA
                     DCTPSNKWIETIIDFYFQQSCDLIIGPVKMKNDSSFFQRLQTLEFVSLIATGATTAAS
                     GKPILCNGANLAFLKKSFLESKNDLHDEELSGDDIFLLQSIKKRQGKIQFLKSTEAFT
                     ETQACKTIAEFFKQRKRWASKSKTYQDKFTVFIALLVFLMCFNILVLLIVSIMQPIFW
                     KIFVTIFIFKYLLDAVFLVTVSKFFSLENVWIGAFFLSLFYPFYMGLVGLAGIVFKQK
                     KW"
     gene            complement(2801..>3049)
                     /locus_tag="PLR72_06870"
     CDS             complement(2801..>3049)
                     /locus_tag="PLR72_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36957.1"
                     /translation="FSGILVYTDFDGKYRAFDLCCPYEAQRDIKIMPNEKGEAVCEKC
                     GSVFFIGYGIGNLISGPAKQPLKRYKAIVQGDVLYIYN"
BASE COUNT         1044 a          474 c          499 g         1032 t
ORIGIN      
        1 ataaaaaaat tatgtcaaaa gtaactgttg ttggtgctgg taatgtaggt gccacttgtg
       61 ttaatgtgct tgcttataac catatagtag atgaagtagt aatgttagat gtaaaaaatg
      121 gcatagcaga aggtaaagct ctggatatga ttcaaactgc tactttgctt cattttaata
      181 caagaattac aggctgtaca aatgattatg ctcaaactgc caattcagat gtagtagtga
      241 ttacttcagg agtacctcgt aaacctggaa tgactcgcga agagctcgta gaggtaaatg
      301 caggaatagt aaaagaagta gctgaaaaaa ttttaaaatt ttcacctaat gctattcttg
      361 tagtagtaag taatcctatg gacacaatga cttatttaac ttacaaagct acaggcttac
      421 caagaaatag agttattggg atgggtggaa ctcttgatac tgcacgtttt aaacattatt
      481 taagtattgc tcttgatgca aatccgaacg aaattgaagc attggtaatt ggagctcatg
      541 gcgatacttc gatgattcct ttggttcgtt atgcttctta taaatgtaga cctgtaactg
      601 aacttttaga taaagacaca ttgaataaag tagtttctga cactatgtta ggaggttcta
      661 caattaccaa tttactgggc acttctgcgt ggtacgcacc aggagctgct gcagcattct
      721 tagtagaagc aatcattcac gacgaaagaa aaataattcc atgttcggta tatttagagg
      781 gtgaatacgg acaaaatgat atttgtttgg gaatacctgc attaattgga aaaaatggta
      841 ttgaaaaaat tctccaatat tcattaaatt atgaagaaag agaattattt aggaaaagtg
      901 cagcttcggt acacgaaacc aatgatactt taaagaaact tgttgaaatt taattcaata
      961 tgatatataa aaatcaagtg gaaaaggttt tgttcaaaaa ctatattttt tcatttgata
     1021 aaattttact aataaatcaa acttctattt ttaatgccga tgccgagagt ttagttagta
     1081 aggactccat tacaaaatct caaaaaactg attattttta ctaattctgt atccattata
     1141 aaaaattatc catgatttat catttcaata gctatcaata tttaatcaag cattctatca
     1201 gaccttctgt tcaacgtaca gcaataatgg actatttaat caacaacaga aaccatccca
     1261 ctgtcgatga aatttatctt gcactggtgc caggaatgcc aactttatca aaaacaacag
     1321 tttacaacac acttaattta tttgtagaac gaggtgccgt acaagtatta aatatagacg
     1381 agaaaaatgc ccgttttgat attaatactt ctatccatgc tcattttatt tgttctcgtt
     1441 gcggaaaaat acaagacatt tttgaaatta gtcccgaaaa tcttgaaata aaagaaaacc
     1501 atgatttcgt tgtagcaaaa gtggaaattt cgtaccatgg aatctgtaaa gattgtttat
     1561 cttcaaatta ataaattttc gctatttttt tatatctgaa aacttagaag tttgatttat
     1621 ccgtcacgaa tttctactaa agatcataaa gttggaaaaa gaaaaattgt atcttttgct
     1681 gcttgagcat ggaattctat atttaccttt tggttatcag tgttttatat ttcctactga
     1741 tcattttttt tataaaaggg tggaaaaaat tgcctgattt cactcctggt gagaccaata
     1801 aaaatatcct ttcagtttct gtcatcgttc cctgccacaa cgaagaaaaa aacttgcttt
     1861 ctttatttga agccttatcc gttcagactt atcaagattt tgaattattg ttgatcaatg
     1921 accattccac tgatgccaca gaaagtatta taaaaaatca tcttccaatt tttaagaatg
     1981 ctgtttattt ttcttcaaaa gaaaaaggta agaaaaaagc cttacaagaa gccattgaat
     2041 tagccaaagg agaattaatt attactactg atgccgattg tactccatca aataagtgga
     2101 tagaaactat tatagatttt tatttccaac aaagttgtga cttaatcatt ggacctgtaa
     2161 aaatgaaaaa tgattcttcc ttctttcaac gactgcaaac ccttgaattt gtttccttaa
     2221 ttgccacagg agcaactact gcagcttcag ggaaaccgat tctttgtaat ggagctaatc
     2281 ttgctttctt aaaaaaatca tttctggaaa gtaaaaatga tttacacgat gaagaattgt
     2341 cgggcgatga tattttttta ttgcaaagca taaaaaaacg acaaggaaag attcaatttc
     2401 taaaatctac ggaagctttt acggaaacac aggcctgtaa aacgattgct gaatttttca
     2461 aacaaagaaa acgttgggct tccaaatcga aaacttatca ggataaattt actgttttta
     2521 ttgcgttgct ggtattttta atgtgcttta atattttagt tttgctgata gtttcaatta
     2581 tgcaacccat tttttggaaa atttttgtta cgatttttat ttttaaatat ttgctggatg
     2641 ccgttttttt agttacggtt agcaaattct tttcccttga aaatgtgtgg ataggcgctt
     2701 tttttctctc gttattttat ccattttata tggggttagt tggattagca ggaattgttt
     2761 tcaaacaaaa aaagtggtga aatttttttt gaaaaattta tcaattatag atataaagca
     2821 catcgccttg aacaatagcc ttataacgtt ttaaaggttg cttggcaggg cctgaaataa
     2881 gattaccaat accataaccg ataaaaaata cactgccaca tttttcacaa acagcttctc
     2941 ccttttcatt cggcatgatt tttatatctc tttgagcttc ataggggcaa cataaatcga
     3001 aagcacgata tttcccatca aaatctgtat aaactaaaat accactaaa
//
LOCUS       DAOSAS010000164         3022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_20925_length_3022_cov_14.096394, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000164 DAOSAS010000000
VERSION     DAOSAS010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20925_length_3022_cov_14.096394"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..841
                     /locus_tag="PLR72_06875"
     CDS             158..841
                     /locus_tag="PLR72_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOK36958.1"
                     /translation="MDIKKSSKASLEDKKSIFILLGLVIALSFLYIGLEWTQREIKVY
                     EDVSTELITEEELDIIQTTQPETPPPPPPPAPEVVEVLNVVENTAETQTINIQTEDIK
                     NKPIDIVAAPVAAPIQEEDEQVIFQVVEEMPHFPGGEQALMKWLSENIRYPVIAQENG
                     IQGRVICQFVVNTDGSIVDIQVVRGVHPSLDEEAVRVIKSMPKWVPGKQRGKPVRVRF
                     TLPINFKLQ"
     gene            947..2149
                     /gene="hflX"
                     /locus_tag="PLR72_06880"
     CDS             947..2149
                     /gene="hflX"
                     /locus_tag="PLR72_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOK36959.1"
                     /translation="MENETYEEIAALVGLITPIQNEEKASEYLEELAFLTETAGAKPQ
                     KIFLQRLNCPDPNTFIGPGKLIEIKSYIIENNINVVIFDDELSARQLRNLEKELNVKI
                     LDRTNLILDIFAKRAKTAVAKTQVELAQYQYMLPRLKRMWTHLERQQGGIGTRGPGER
                     QIETDRRIILSKIATLKKKLKEIEQQTITQRKNRGKLVRVALVGYTNVGKSTLMNLLS
                     KTDLLAEDKLFATVDTTVRKVIIHNLPFLLTDTVGFIRKLPHQLIESFKSTLDEVREA
                     DLLLHIVDISHPNFEEQIQIVNQTLKEICGEEKPMILVFNKIDAFPYKPKEADDLSPL
                     QHESISLEELEKSWMAKINEDCVFISAKEKQNIAKLKELMYKKIKAIHIQRYPYNDFL
                     YPDDLSAN"
     gene            2184..>3022
                     /locus_tag="PLR72_06885"
     CDS             2184..>3022
                     /locus_tag="PLR72_06885"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOK36960.1"
                     /translation="MKTYRQLTKKEINSLTLQGCIAENWNQIKVTPDFNSQFVVNVHF
                     SGEIFLEGFQKIFELPGGVKKHAGIYNCCLHNVMVEKDVYINRIHNYIANYTIGEGSY
                     IENVNLLVVDGWSTFGNGVRVPVMNEGGGREIPIFDYLSAPLAYILTLYRHRPTLIQN
                     LQKLIDDYASSIGSSKGSIGQNVHIINCGTIRQVRIGNYANIEGASVLFNGSINSSAL
                     APITIGAGVKCNDFIISSGVSITDSTLVSRCFIGQGCILGKHYSALDSLFFSNCQGMH
                     GEAT"
BASE COUNT         1100 a          475 c          524 g          923 t
ORIGIN      
        1 aaaaaaagta catctaaaaa taaatatttc aagagaaatg aataattttg ccatagaaga
       61 aaaaataaat attttttctt tattaattat ataaacattt taaaaaaaaa ttgtacattt
      121 gctccatctg aaaaatttat aaaaagacct tataattatg gatatcaaaa aatcgtctaa
      181 agcttcatta gaagacaaaa aatcaatttt tattttattg ggtttagtaa tagctttatc
      241 ctttctttac ataggattgg aatggacgca acgtgaaatt aaagtttatg aagatgtaag
      301 tactgagtta attaccgaag aagaactcga tattattcaa acaacacagc cagaaactcc
      361 tccccctcct ccacctcctg caccagaagt agtagaagta ttaaatgttg tagaaaacac
      421 tgctgaaaca caaacaatta acattcagac agaagatata aaaaataaac cgattgatat
      481 tgtagcagca ccagtcgcag ctcctataca agaagaagat gaacaagtta tcttccaggt
      541 agttgaagaa atgccacatt tcccgggagg tgaacaagct ttgatgaaat ggttgagtga
      601 aaatatcagg tatccagtta ttgctcaaga aaatggaatt caaggacgtg taatttgcca
      661 atttgtagta aacactgatg gttcaatagt tgatattcaa gtggtaagag gtgttcatcc
      721 aagtctggat gaagaagcag tgcgagttat taaatctatg cctaaatggg ttccagggaa
      781 acaacgtgga aagcccgtac gagtaagatt cactcttcct atcaacttta aattacaata
      841 attattaatc gagataggaa gttagtctta tgatttaata ttgacttcga aaaatttaaa
      901 accgtttggt agctccaaac ggttttttaa taaaggaatt tcaactttgg aaaacgaaac
      961 atatgaagaa atagcagcgt tagtcggact gataactccg atccaaaatg aagaaaaagc
     1021 atccgaatac ttggaggaac tagccttttt aaccgaaaca gcaggagcca agccacaaaa
     1081 aatttttctt cagcgtctca attgtcctga tccaaatact tttattggtc caggaaaatt
     1141 gatagaaata aaatcatata tcattgagaa taatattaat gtagttattt ttgatgatga
     1201 attaagcgca agacaactcc gaaatctgga aaaagaactc aatgtaaaaa ttttagacag
     1261 aactaattta attttagata tttttgctaa acgtgctaaa acagcagttg ccaaaactca
     1321 agttgaattg gcacaatatc aatatatgtt acctcgttta aaacgtatgt ggacacacct
     1381 ggaacgacaa caaggaggga ttggcactcg cggtcctggt gaaagacaaa tagaaactga
     1441 tcggcggatc attctttcaa aaattgcaac tttaaagaaa aaattaaagg agattgagca
     1501 acaaaccata actcaacgaa aaaatagagg aaaattagta cgtgtcgctt tagtaggcta
     1561 tacgaacgta ggaaaatcaa ctttaatgaa tttacttagc aaaacagatc ttctggcaga
     1621 agataaactt tttgctacag ttgatactac ggtacgaaaa gtaattattc ataatttgcc
     1681 ttttttactt actgatactg ttggatttat ccgtaaatta cctcatcaac ttattgaatc
     1741 gttcaaatca actttagatg aagtacgtga agctgacttg ttattacata ttgtggatat
     1801 ttcccatcct aattttgaag aacaaattca aatagttaat caaacactga aagaaatttg
     1861 cggtgaggag aaacccatga ttttagtttt taataaaatt gatgcttttc cttataagcc
     1921 gaaagaagct gacgatcttt cacctttgca acatgaaagc attagtctgg aagaattgga
     1981 aaaaagctgg atggctaaaa tcaatgaaga ttgtgtattt atttctgcaa aagaaaaaca
     2041 aaacattgcc aagctaaagg agttaatgta caaaaaaata aaagctattc acattcaacg
     2101 ttatccgtac aatgattttt tatatccaga tgatttatct gcaaactaaa aaataacaaa
     2161 ccaattccta ataactttta actatgaaaa cttatcgtca acttactaaa aaagagatta
     2221 attcgctcac cttacaaggt tgcattgcag aaaattggaa tcaaataaaa gttactcccg
     2281 atttcaatag tcaatttgtt gtaaatgttc atttttcagg agaaatattt ttggagggct
     2341 ttcagaaaat ttttgaactg cccggtggag tcaaaaaaca tgctggcatt tataattgtt
     2401 gtcttcacaa tgtaatggtt gaaaaagatg tttacattaa cagaattcat aactacattg
     2461 ccaattatac tattggtgag ggttcgtata ttgaaaatgt caatttatta gtggtagatg
     2521 gttggtcaac ttttggaaat ggtgtaaggg ttcctgtaat gaatgaaggt ggaggacggg
     2581 aaattcccat ttttgattat ctatccgctc cattagctta tattttaaca ctttatcgtc
     2641 atcgacctac cttaattcag aatcttcaga aactaataga tgactatgct tcttctatcg
     2701 gttccagtaa aggttcaata ggacaaaatg ttcatatcat aaattgtggc accattcggc
     2761 aagttagaat aggaaattat gccaacattg aaggagcttc agtattattc aatggaagta
     2821 ttaatagcag cgcattagct cctatcacta ttggtgcagg tgttaaatgc aatgatttta
     2881 ttattagttc tggagtttct attaccgatt ccacgttggt ctctcgctgc tttataggac
     2941 aaggctgtat tttgggcaaa cattattcag ctcttgactc acttttcttt tcaaactgtc
     3001 aaggaatgca cggtgaagca ac
//
LOCUS       DAOSAS010000165         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_21079_length_3005_cov_10.262712, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000165 DAOSAS010000000
VERSION     DAOSAS010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21079_length_3005_cov_10.262712"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PLR72_06890"
     CDS             complement(<1..628)
                     /locus_tag="PLR72_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOK36961.1"
                     /translation="MNLSYHWLQQYINIHLPVDELAEILTSIGLEVDSYEEISSVKGG
                     LEGLVIGEVLTCQPHENSDHLFVTTVNIGEVEPLQIVCGAPNVAAGQKVVVAPVGTKL
                     YAGEETFIIKRSKIRGVESYGMICAEDEIGIGTSHEGIIVLPDDTVPGTPAKEYYKIE
                     NDVVFEIDLTPNKADATSHFGVARDLAAYFSIRDKAIHLTKPSVENFKV"
     gene            905..994
                     /locus_tag="PLR72_06895"
     tRNA            905..994
                     /locus_tag="PLR72_06895"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:939..941,aa:Ser,seq:gct)
     gene            1184..2548
                     /locus_tag="PLR72_06900"
     CDS             1184..2548
                     /locus_tag="PLR72_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOK36962.1"
                     /translation="MEKTKKIILPEKEIPFQWYNIVAELPNKPMPILHPATKQPIKEE
                     DLYPIFAKELSHQELNQTDLWIDIPEEVREKYKIYRPTPLIRAYELEKALDTPAHIYY
                     KNESVSPAGSHKLNTALAQAYYCKKEGITNITTETGAGQWGTALALAAKFFGLELTVY
                     MVKISYEQKPYRRSLMQLWGAQVIPSPSNTTKAGQKILAERPNYMGSLGTAISEAIEL
                     ATEKPNCKYTLGSVLNHVSLHQTVIGLEAEKQMEIAGEYPDIVIGCFGGGSNFSGISF
                     PFIRHNIRNGKKTRFIAAEPTSCPKLTRGIFEYDFGDEAGYTPLLPMFTLGHNFAPAH
                     IHAGGLRYHGAGTIVSQLMKDNLMEAVDIDQLDSFKAGLLFAQTEGIVPAPESAHAIA
                     ATINEALKAKEEGTPKTILFNLSGHGFLDMSAYDQYLSGELTNYTLTEEEITRNVNKL
                     EHLV"
     gene            2681..>3005
                     /locus_tag="PLR72_06905"
     CDS             2681..>3005
                     /locus_tag="PLR72_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36963.1"
                     /translation="MKRKIILFSFISAVFILFFTGCISKTVKTKILEYSNKFYLTSDT
                     TKGGLSLSIQVEMPVKFHNKNVLDTIRKQLISRIFGEEYQSLPLDSVIPAFANSQRDD
                     YLENNL"
BASE COUNT         1042 a          498 c          469 g          996 t
ORIGIN      
        1 gtactttaaa attttcaacg gaaggttttg ttaaatgaat ggctttatcc ctgatggaga
       61 aataagcagc cagatcacgg gctactccaa aatgagaagt agcatctgct ttatttggcg
      121 taagatctat ttcaaaaaca acgtcatttt caattttgta atactctttt gctggcgtac
      181 caggaactgt atcatctggc aaaacaatta ttccttcgtg actggtgcca ataccaattt
      241 cgtcttcggc acaaatcatc ccataagatt caactccacg aattttagaa cgcttaatga
      301 taaaagtttc ttcacctgca taaagttttg ttcccactgg tgcaacaact accttttgtc
      361 ctgcagccac attgggagct ccgcaaacaa tttgcaaagg ttcaacttca ccaatgttga
      421 ctgtcgtcac aaataaatga tcagaatttt catgcggctg gcaagttaaa acttcaccaa
      481 ttaccaatcc ttccaaacca cctttcacag aagaaatttc ttcataacta tccacttcca
      541 agcctataga agttaatatc tctgctaatt catcaacagg aagatgaata ttaatatact
      601 gctgtaacca atgataagat aaattcataa tttacaattt attacaatca acccaaccgg
      661 gttttgttcc attttttata gtttgcaaag atacgttttt taaaagacat ataaaatttg
      721 atttttatca tagctatatg aagatttcgg tttttgaatg aagttatcct tatagctaaa
      781 ttttttgcta agattgagca aaattgatat tgatttttaa aatttgacga aaaagcctta
      841 acaaaaagaa gaaaagactt tgaaaaatga aaacactttt ttaactttgc attgaaaaat
      901 aaaaggaagt gtgggtgagt ggctgaaacc atcagtttgc taaactgacg tacgggcaac
      961 cgtaccacga gttcgaatct cgtcgcttcc gccataaaaa ttaattaatt acttatcaaa
     1021 cgctttattt ttaaattctt accttaattt tttattccct tcttctataa ataaattttg
     1081 ttagaaaaac atcttttttt taaacattca aatgtttgta gtatctttgg atgctcaaat
     1141 ttatattaat ttacaataaa ttattacaat aaattaatta tttatggaaa agacaaaaaa
     1201 aattatttta cctgaaaaag aaataccttt ccagtggtat aatattgttg cagaactacc
     1261 aaataaaccc atgcctattc ttcatcctgc aacaaaacaa cctattaaag aagaagattt
     1321 atatcctatt tttgcaaagg aattatctca tcaagaactt aatcaaactg atttatggat
     1381 tgatattcca gaagaagtac gagaaaaata taaaatttat cggcctaccc ctttgataag
     1441 agcttatgaa ttagaaaaag ctttagatac tcctgcccat atttattaca aaaatgaaag
     1501 tgtcagccca gcaggttcac ataaactaaa tactgcttta gctcaagctt attattgtaa
     1561 aaaagaaggg attaccaaca ttaccactga aactggtgct ggacaatggg gaacagcttt
     1621 agctcttgct gctaaatttt ttggtttgga attaactgtt tatatggtaa aaattagtta
     1681 tgaacaaaaa ccttatcgtc gttcattgat gcaattgtgg ggagctcagg taatcccttc
     1741 cccaagcaat accactaaag ctggacaaaa aattttagct gaacgtccaa attatatggg
     1801 cagtcttgga acagccattt ctgaagcaat agaacttgcc actgaaaaac caaattgtaa
     1861 atatactttg ggaagtgtcc ttaatcacgt ttcgcttcat caaactgtaa tagggttaga
     1921 agctgaaaaa caaatggaaa tagcaggaga atatcctgat atagttattg gatgttttgg
     1981 cggtggttca aatttttcgg gaatttcttt tccgtttatt cgtcacaata ttagaaatgg
     2041 taaaaaaaca cgttttatag ctgctgaacc tacttcttgt ccaaaattaa caaggggaat
     2101 atttgaatat gactttggtg atgaagctgg ttatacacct cttttaccca tgtttacctt
     2161 aggacataat tttgctcctg cacatattca tgcaggcgga cttcgttatc atggtgccgg
     2221 cacaattgta agtcaattaa tgaaagataa tttaatggaa gcagttgata ttgatcaact
     2281 tgattcattc aaagccggtt tgctctttgc tcagacagaa gggattgttc ctgcaccgga
     2341 atcagcacat gctattgctg ctactattaa tgaagcttta aaagcaaaag aagaaggaac
     2401 tccaaaaact attttattta atttatcagg acatggattt ttagatatgt cagcctatga
     2461 tcaatattta tctggtgaat taactaatta tactttaaca gaggaagaaa ttaccagaaa
     2521 tgttaacaaa ttagaacatc tggtttaata attggtaata tttcaaagca agtacaaact
     2581 tattttgttt gtcttgcttt tttcaaatta taagttagca aaaaagtaag ttgtttttgt
     2641 ttattatcgt aattttgtcg atgaaaaaaa tctacaaaaa atgaaaagaa aaattatttt
     2701 attttctttt attagtgcgg tttttatttt gtttttcaca ggttgcattt caaaaacagt
     2761 gaaaacaaaa atattggaat attccaataa attttattta acttctgata ctaccaaagg
     2821 aggattatcg ctttccattc aggtagaaat gccagtaaaa tttcataata aaaatgtatt
     2881 ggatactatt cgtaaacaac tcatttccag aatatttgga gaagaatatc agtcattgcc
     2941 acttgattca gtaattccag cttttgcaaa tagccagcga gatgactatt tggaaaacaa
     3001 tttag
//
LOCUS       DAOSAS010000166         3004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_21092_length_3004_cov_9.560190, whole genome shotgun sequence.
ACCESSION   DAOSAS010000166 DAOSAS010000000
VERSION     DAOSAS010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21092_length_3004_cov_9.560190"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLR72_06910"
     CDS             complement(<1..433)
                     /locus_tag="PLR72_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36964.1"
                     /translation="MSGFVLDFVGIFDHLEKALAFDSQDIAGIVEDVEELKKRFKEEM
                     EKARKNYLTIIGGKKQDKAVETLLNYFLEEEIRNEYYQFFKELSTIYEIISPDAFLRD
                     YLEDYETLSRMYQILRENYDRGIDINKDFTRKTIELVKKHTK"
     gene            complement(576..2717)
                     /locus_tag="PLR72_06915"
     CDS             complement(576..2717)
                     /locus_tag="PLR72_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOK36965.1"
                     /translation="MILLNIFEITLPLTNPVLKFLVILIIILFAPILLNKLKIPHILG
                     LIIAGAVIGPNGFNLLLRDSSIILSGTVGLLYIMFLAGLEIDLAEFKKNSAKSLLFGL
                     YTFLIPMTLGTFAGIYILNFSILTSVLLASMFASHTLIAYPILSKLGVTKNRAVNIAV
                     GGTMITDTLALLVLAVIVGMAHGEVNTAFWTKLSISILVFGLIVMFLFPIIGRWFLKR
                     YEDNVSQYIFVLVMVFLGATLAEAAGVEAIIGAFLSGLALNRLIPSTSSLMNRIEFVG
                     NAIFIPFFLIGVGMLVDYRAFFKDFETIKVAAVMTIVATSAKYIAAWLTQKSFGFSVD
                     ERRLIFGLSNAQAAATLAAVLVGYNVIIGENANGEPIRLLSESVLNGTIIMILITCTI
                     ASFVAQKGGKNISLQVSSTVESEDVEQTEKILIPLSNPETMEELINLSLIIKSKENNK
                     GLFALVVINNDSSETYNHAKKLLDKAVFTASANDIQLNKILRYDINTTNGIIGVIKEQ
                     NISDLVLGLHVSKGISESFLGKLTERILTKCNTTTFVYKPIQPISTIKRHVVIVPQNA
                     EKEIGFPFWLSKIWNIAKNTGAKIVFYAHQTTLAYIKKIYLKHPIEADFKIFDDWNDF
                     LIISREIKIDDNLLIVLSRKNKLSYHENMTNIPTYLNKYFQTNSFILVYPMQQGVVDN
                     EEIDLNNPSLIEPIEKLDEIGKNIMKLFRRK"
BASE COUNT          962 a          555 c          480 g         1007 t
ORIGIN      
        1 ttttcgtatg cttttttacc agttcaatcg tttttcgggt aaaatcttta ttgatatcaa
       61 taccccgatc gtaattttct ctcaaaattt ggtacattct cgaaagcgtt tcgtaatcct
      121 ccagataatc ccgcaaaaaa gcatcgggtg aaataatttc gtaaatggtt gatagttcct
      181 tgaaaaactg atagtattcg ttcctgattt cttcttccag aaaataattc agcaaagttt
      241 ccactgcttt atcttgcttt tttccaccga taatggtcag gtaatttttc cttgcttttt
      301 ccatctcctc tttgaacctc tttttcagct cttctacatc ttccacaatg ccggcaatat
      361 cctgcgaatc gaatgcaagg gctttttcca gatgatcgaa aatgccaaca aaatcgagta
      421 cgaaacccga cacttttttt ctcccttctt catcttcata gggacgattg acgcgggcaa
      481 tttcctgtca agttacaact acttttgatg caagctgaaa cgctcgataa tacactaact
      541 gcaaaatttt ttatataaga cgttgggtgc tgggcttatt ttcgtctgaa tagtttcata
      601 atatttttcc ctatttcatc aagtttttca attggctcaa ttaaagatgg attattcagg
      661 tctatctctt cattatcaac aactccttgc tgcataggat aaacaagaat aaaactattt
      721 gtctgaaaat atttattcaa atatgttggg atattagtca tattctcatg atacgatagt
      781 ttattttttc tgcttaaaac tattagtaaa ttatcgtcaa ttttaatttc tcgtgaaata
      841 attaaaaagt cgttccaatc gtcaaaaatc ttaaaatccg cttcaatcgg gtgttttaaa
      901 taaatttttt taatgtatgc aagagttgtt tggtgtgcat agaaaacgat ttttgcacct
      961 gtatttttgg cgatattcca tattttggac aaccaaaatg gaaaacctat ttccttctca
     1021 gcattttgag gtacaattac aacatgcctt tttattgtag aaataggctg tattggtttg
     1081 tatacaaatg tagtcgtatt gcatttagtc aaaatccgtt ccgttaattt accgagaaat
     1141 gattctgaaa tacctttgct tacgtgaagc cctaaaacta agtcactaat attttgttct
     1201 ttaataactc cgataattcc atttgttgta tttatatcat atctcaaaat tttgtttaat
     1261 tgtatgtcat tggcagaagc agtaaaaaca gccttgtcta atagtttttt tgcatgatta
     1321 taagtttccg aagagtcatt attaataaca acaagagcaa ataatccctt gttattttct
     1381 tttgatttaa ttattaaact tagattaatt aattcctcca ttgtttcggg attgcttaat
     1441 ggaattaata ttttttctgt ttgttcgaca tcttcgcttt caaccgttga tgatacttgc
     1501 aacgaaatat ttttgccccc tttttgagct acaaaagatg ctatagtaca agtaatgagt
     1561 atcattataa ttgttccgtt taagacgctt tcgctcaata aacgaattgg ttctccgttg
     1621 gcattttcac caattatgac attataccca actaacacag cagcaagtgt agcggctgcc
     1681 tgtgcattac ttaatccaaa gattaatcta cgttcatcaa ctgaaaaccc aaaagatttt
     1741 tgagtaagcc atgcagcgat gtattttgct gaagttgcta ctattgtcat tacggcagcc
     1801 acttttatag tttcaaaatc tttaaagaaa gcccgatagt caacaagcat tcccactcca
     1861 ataagaaaaa atggtataaa aatcgcattc cctacaaatt caatcctgtt cattagcgaa
     1921 gacgtagaag gaattagacg gttcagtgcc aaccctgata aaaatgcacc aataatcgcc
     1981 tcgacacctg ctgcctcagc aagcgtagca ccgagaaaaa ccattacaag aacaaaaata
     2041 tattgagaaa cattgtcttc gtatcttttg agaaaccaac ggccaatgat tggaaaaaga
     2101 aacataacta ttaaaccaaa aactaaaata gagattgaaa gtttagtcca aaaggcagtg
     2161 ttaacttctc cgtgagccat accaacgata acggcgagaa ctaaaagagc aagagtgtcg
     2221 gtaatcattg taccaccaac agcaatgttt accgcacgat ttttcgttac acctaatttg
     2281 ctaagtattg ggtaagcaat cagtgtatgt gaagcaaaca tactggcaag taagacggat
     2341 gtaagaattg aaaaatttaa aatataaata ccagcaaagg tacctagtgt cattggtatt
     2401 aaaaaagtgt acagtccgaa cagcaaactt ttagcactgt tctttttgaa ttctgccaaa
     2461 tcaatctcca agcctgcaag aaacataata tacaacaagc ctactgtccc tgataaaata
     2521 atgctactgt ctcttaataa tagattaaaa ccattcggtc caataactgc tcctgctata
     2581 ataagaccaa gaatatgcgg gattttcagt ttatttaaaa gaatcggggc aaataatata
     2641 ataatcagaa ttaccagaaa tttgagtact ggattagtca acggtagggt tatctcaaat
     2701 atatttaata aaatcataat ctgtcaattt taaagttttt tctgcaaata tatgaaatgc
     2761 tgtcggtttt tttgtctctc tgccttgcgt ccaacgaccg aggctaagca cagtagccaa
     2821 tttgcgggct acttcattgt tgcactacga tgaaggtaat gcgggagata aacctccaaa
     2881 attgtatgaa ttcggctatt gtcgcttaga cgtgtgttac caaatgtgca ttggttgttt
     2941 gaaaattatt tgctgaattc aagttacaaa tgcaactttt tggttagaca aatttcgtaa
     3001 taaa
//
LOCUS       DAOSAS010000167         2972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_21432_length_2972_cov_16.447035, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000167 DAOSAS010000000
VERSION     DAOSAS010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21432_length_2972_cov_16.447035"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..1790)
                     /locus_tag="PLR72_06920"
     CDS             complement(216..1790)
                     /locus_tag="PLR72_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOK36966.1"
                     /translation="MNSELVIDVKPSEISIALLEDKHLVELTHESREEQFSVGNIYLG
                     KVKKIMPGLNAAFVDIGYEKDAFIHYLDLGPNFNTLSHYTTQVLNERKKFPFLQKIKV
                     FPEIEKNGSINDVLKPGQEILVQISKEPISTKGPRLTSEISIAGRFLVLIPFNDKVMI
                     SQKIKSENERARLKQLIQSIKPKGFGVIVRTVSEEKKVAELNNELNILVKRWEEIVNK
                     LQQSRGVTLLSEELGRAVGIIRDLFSPNFNNIYVNDPEVFNEIRDYIELIAPEQKDIV
                     KLYKGEIPIFDNFEITKQIKSSFGKTVSLKNGAYLIIEHTEALHVIDVNSGNRSKAGS
                     DQEANALEVNLAAVDEIARQLRLRDMGGIIVIDFIDLHNAENRQKVYERMQEMLANDR
                     AKHNILPLSKFGLMQITRQRVRPETHIDIEETCPTCFGTGKIKPSILFTDQLESKIDY
                     LVNELNIKEFTLQVHPYVEAYISKGLFPLKWKWKQKYGKGCKVIASQNLGFLQYKFFD
                     KKGNEIDLHEEYEKFS"
     gene            complement(2009..2281)
                     /locus_tag="PLR72_06925"
     CDS             complement(2009..2281)
                     /locus_tag="PLR72_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOK36967.1"
                     /translation="MTKAEIVNEIAKTTGIDKNTVLATVEAFMETVKDSLAKNENVYL
                     RGFGSFIVKKRAKKTARNISKNTTIIIPEHNIPAFKPAKAFMNVVK"
BASE COUNT          927 a          447 c          401 g         1197 t
ORIGIN      
        1 taaaaataga actttcgaat ctttgaaaga tgttgaagat acattagcag aacaaatgaa
       61 taactacgag gcaagcctcg aggaattctt ttgattaaaa aatctggaca aagaagcggt
      121 aaaatcagtc ttcattttaa aactgatttg ctacaaaatt ttgataaaga aacaggatga
      181 ttgaaaaaat catcctgttt cttatttttt acttgttacg aaaatttttc atattcttca
      241 tgaaggtcaa tttcattccc ttttttgtcg aaaaatttat attgtaaaaa acccagattt
      301 tgggaagcaa ttactttgca acctttgcca tatttttgtt tccatttcca ttttaaagga
      361 aataatcctt ttgaaatata ggcttccaca taaggatgaa cttgtagagt aaattcttta
      421 atatttagct cgttaactaa ataatcgatt ttactttcta attggtcagt aaataaaatg
      481 gaaggtttaa tttttcctgt accaaaacaa gtaggacacg tttcttcaat gtctatgtgt
      541 gtttcaggtc ttacacgttg tcttgtaatt tgcattaaac caaatttgct taatggaagg
      601 atattatgtt tagctctatc attagcaagc atttcttgca tcctttcata tactttttgc
      661 ctattttcag cattgtgtaa atcaatgaaa tcaatgacaa taattcctcc catatctcgt
      721 agtcgtaatt gacgggcaat ttcatcaaca gcagctaaat ttacttccag tgcatttgct
      781 tcttgatcac tgcctgcttt cgaccgattt ccgctattta cgtcaataac gtgtaatgct
      841 tctgtatgtt caataatgag ataagctcca tttttaagcg aaactgtttt cccaaaggaa
      901 gatttaattt gtttagttat ttcaaaattg tcaaatattg gaatctcacc tttgtaaagt
      961 ttaactatat ccttttgttc gggtgctata agttcgatat aatcgcgtat ctcattaaaa
     1021 acttcaggat catttacata tatgttatta aaatttggac taaataaatc tctaattatt
     1081 ccaactgctc gacctaattc ttctgataac aacgtaactc ccctggattg ttgtagctta
     1141 tttacaattt cttcccatcg ttttactaaa atatttaatt cattattgag ttcagctact
     1201 tttttttctt cagaaacagt tcttacaatt acaccaaatc cttttggctt tatgctctgg
     1261 attagttgct ttaagcgtgc tctttcgttt tctgatttga ttttttgaga aatcattact
     1321 ttatcattaa aaggaattaa taccagaaaa cgtcctgcga tagagatttc tgaagttaat
     1381 cgtggtcctt ttgttgaaat aggttctttt gaaatctgaa ctaatatctc ttgtcccggt
     1441 ttaagaacat cattaataga accatttttt tcaatttctg ggaagacttt tattttctgt
     1501 aaaaaaggga attttttcct ttcgtttagt acttgtgtcg tgtagtgaga aagggtattg
     1561 aaatttggac ctaaatcaag ataatgaata aaagcatctt tctcgtaacc gatatcgaca
     1621 aatgctgcat ttaaaccagg cataattttt tttacctttc ctaaatagat atttcctact
     1681 gaaaattgtt cttctcgcga ttcatgagtt aattctacca ggtgtttatc ttcaagcagc
     1741 gcaatagaaa tttccgatgg tttcacatcg attactaatt cactattcac tttcttaatt
     1801 tattttgatt ataatataac aaaaaacaaa cttaaagccc ctttgctttt aagtttgttt
     1861 tttggacttt aatgactaaa gaccaaggtc ttatttcttc ttatgtctat tttttctcag
     1921 tctttttttt cgtttatggg tagacatttt atgtcttttc ctttttttcc cgcttggcat
     1981 tattaatact gcttttactg ttaaattttt attttacaac attcataaaa gcttttgcag
     2041 gcttaaatgc aggaatgttg tgttctggaa taataatggt cgtgtttttt gaaatgttac
     2101 gagctgtttt tttagctctt tttttcacga tgaaacttcc aaatcctctg agataaacat
     2161 tctcattttt tgctaacgaa tcttttactg tttccataaa agcttcgacg gtagccaaaa
     2221 ccgtattttt atctattccg gttgttttgg caatttcatt tacaatttct gcttttgtca
     2281 ttgtagtaat atttttaaat gagttaataa attatttaat taattgatat taaattcaaa
     2341 tagaattgca aatatatctc ttttattctg attaaaaaac atttggaaca ttttttttct
     2401 tttaaatttt atttctttat taatagtcag acgcataata ttgttttaaa aatttaaatc
     2461 ttgagtcccc tcttaaaact taattttttc aaaagtaaga aaaaatgaga tttttttgaa
     2521 gaaaagtatt aaatttttca ttgtttagat attttacaat catttgcaat ctaaaagtta
     2581 tttttactaa aaaattagct ttttctaaag tgtcaaacga tctttaacca tcttgtatga
     2641 tatatttcag tgtttggact aagtttatct atatagttga acaagaataa gcctataatt
     2701 tggtgtttgg caagttcacg ataatggagt ttgagcaaag ctcctaatat aaatgtttta
     2761 ttccccaaaa aattttatgc aattaaaaat ttttgtcata cacttagttt tttctaacag
     2821 tgattttatg aacaaattaa ttattaggaa cctttttaat tataaaaaat tcttggaata
     2881 aacgcattta tttttaaaaa ataataattt acaaatgaaa gtagaaatat ttccatatta
     2941 tcttgaattt tttaaactat ttttgtgttg aa
//
LOCUS       DAOSAS010000168         2952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_21633_length_2952_cov_13.822920, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000168 DAOSAS010000000
VERSION     DAOSAS010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21633_length_2952_cov_13.822920"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..2343
                     /locus_tag="PLR72_06930"
     CDS             145..2343
                     /locus_tag="PLR72_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOK36968.1"
                     /translation="MSSLRFKAVEEAFKKKAINVEPPSALVSDYFGKYVFTRSKMAEY
                     LSEETMKAIDEAIEKGVTLDRSIADDVAAGMKKWAIELGATHYTHWFQPLTEKTAEKH
                     DSFIDYVEDKPCEKVIEKFSGKLLAQQEPDASSFPSGGIRSTFEARGYTAWDPSSPAF
                     VMDDTLCIPTIFISYTGESLDYKAPLLKALNAVDKAATAVCQLFNSKVKKVYSYLGWE
                     QEYFLVDENLYAARPDLMLTGRTLLGHESAKNQQLEDHYFGAIPTRVMAYMKDLEFEA
                     YKYGIPLKTRHNEVAPNQFELAPIYNECNLSVDQNLLLMSIMKKVARRHSFRLLLHEK
                     PFAGINGSGKHCNWSLGTDIGMGLFTPGKTPEENLLFITFVVNVLMAVYKNNSLIKAS
                     IMTAQNAHRLGANEAPPAIISVFLGEQLSNILDKLEMSDSEDIIIFDHKKKLNLKIPQ
                     IPEVLLDNTDRNRTSPFAFTGNRFEFRAMGSSANCSTAMIVLSTVLAAQLLDFKSEVD
                     TKMEAGLAKQQAIFEVIKEYIQISKPIRFEGNNYSEEWKVEAKKRGLDCEMSVPKILK
                     AYLKPESVHIFSFLGVLSEKELYARNEVKLETYTKKIQIESRVLGDLAVNHIIPVAIR
                     YQTLLAENISKIKNITFDNNGKDICKMQLELIREIAQRVNIIKENVNEMIEARKKANI
                     IENEEEKANAYHDTVLPYFDVIRYHIDKLEMIIDDEMWPLPKYREILFIR"
     gene            2423..>2952
                     /locus_tag="PLR72_06935"
     CDS             2423..>2952
                     /locus_tag="PLR72_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36969.1"
                     /translation="MENNDWKEKTQQFQKEIEKEIAELEVTMKQLMDGLNEKYGSQVE
                     NLKQQAKATQAKIDEKIAQIQAKMNDSTDEMKRKYADQITELKQKEELIQKEVDKKIA
                     ELGKKMSESADELKTKYEEQIKELKKMKDDLLYKMNNWSEVTEKNFRALTEVNEKTFK
                     EISQTLKQSFSEIKNL"
BASE COUNT         1120 a          405 c          551 g          876 t
ORIGIN      
        1 aatgagtttt tcaaacattt tttataattt cgcacttgct ggttgactct actctaatga
       61 ttttttattc cgaaatttgt caaataaaaa acaatatcat tattttgtaa gtttgtttat
      121 tacaattata taacatacaa gtttatgtca tcattaagat ttaaagcagt agaagaggct
      181 tttaagaaaa aagctattaa tgtagagccg ccatcggcat tggtttccga ttattttgga
      241 aaatatgtat ttactcgttc aaaaatggca gaatatcttt cagaggaaac catgaaagct
      301 attgatgaag ccattgaaaa aggagtaacg cttgatagaa gcattgctga tgatgtggca
      361 gcagggatga aaaaatgggc tatcgaatta ggagctaccc attatactca ttggtttcag
      421 ccattaacgg agaaaactgc agagaaacat gattctttta ttgattatgt agaggataaa
      481 ccatgtgaaa aagtaattga aaaattttca gggaaattat tggcacaaca ggaacctgat
      541 gcttcttctt ttccgagtgg cggtattcga agtacttttg aagcacgagg ttatactgcg
      601 tgggatcctt cttcgcctgc ttttgttatg gacgacacac tgtgtattcc aaccattttt
      661 atttcttata caggagaatc acttgattat aaagcgccac tgttaaaggc tttaaatgct
      721 gttgacaaag ctgcaactgc tgtatgtcag ttgtttaact caaaagtcaa gaaagtttat
      781 tcatatttgg gctgggaaca ggaatatttc ttagtagatg aaaacttgta tgctgctcgt
      841 cccgatttaa tgttaacagg acgaacatta ttgggacatg agtcagctaa aaatcagcaa
      901 cttgaagacc attattttgg agcaattcct acacgtgtaa tggcatacat gaaagatctg
      961 gaatttgaag cttataaata tggtattcca ttaaaaacac ggcataatga agttgcccct
     1021 aatcaatttg aactagcccc catttataat gaatgcaact tgtcagttga tcaaaattta
     1081 ctattaatgt caataatgaa aaaagttgca cggcggcata gtttcagatt actcttgcac
     1141 gagaagcctt ttgctggaat aaatggttcg ggcaaacatt gtaactggtc attgggtacc
     1201 gatatcggaa tgggattatt cacacccgga aaaactccag aagaaaatct tttgtttatc
     1261 acttttgttg tcaatgtttt aatggcagtt tataaaaata attcattgat taaagcttcg
     1321 atcatgactg ctcaaaatgc tcaccgatta ggagcaaacg aagcacctcc agctattata
     1381 tcggtatttt taggtgaaca actttcaaac atactggata aactggaaat gagcgatagc
     1441 gaagacatca ttatttttga tcataagaaa aaattaaatt taaaaattcc tcagattcct
     1501 gaggttttat tagacaatac agacaggaac agaacttcac cttttgcctt tacaggaaat
     1561 cgttttgaat ttagggcaat gggctcgtca gccaattgtt ccacagctat gattgttctc
     1621 agcacagttt tagcagctca actgcttgac tttaaatcag aagtggatac aaaaatggag
     1681 gcgggattag ctaaacaaca agcaattttt gaagtaataa aagagtatat acaaatttcc
     1741 aaacctattc gttttgaggg aaataattat tcggaggagt ggaaagttga agcaaaaaaa
     1801 agaggtttgg attgtgagat gagtgttcct aaaatattaa aagcttatct caaaccggaa
     1861 agtgtccata ttttttcttt tttgggtgtt ttatcggaaa aagaacttta tgctcgtaat
     1921 gaggtaaaac tcgaaacata tacgaaaaaa attcagattg aatccagagt tttgggtgat
     1981 cttgctgtaa atcatattat ccccgttgct attcggtatc aaactttatt ggcagaaaat
     2041 atttctaaaa tcaaaaatat aacttttgat aacaatggaa aagatatttg caaaatgcaa
     2101 ctggaattaa ttcgtgaaat tgctcaacga gttaatatta ttaaagaaaa tgtaaatgaa
     2161 atgatagaag caagaaaaaa ggcgaatatt attgaaaatg aagaagaaaa agcaaatgct
     2221 tatcacgata cggtattgcc ctattttgat gtaatacgtt accatattga caaattggaa
     2281 atgattattg atgatgaaat gtggcccttg ccaaaataca gagagatact gtttattaga
     2341 taggagaaga ataggtataa aaaatgagta agttattata ataaaaaatt gtaaaaatgt
     2401 ttcatttaat attatagaaa atatggaaaa caatgattgg aaagaaaaaa cgcagcaatt
     2461 tcagaaagaa attgaaaaag aaattgctga attggaagtc acaatgaaac aattgatgga
     2521 tggtttgaat gaaaagtatg gatctcaagt ggagaattta aaacagcagg caaaagcaac
     2581 tcaagcaaaa attgacgaaa aaattgcgca gattcaagct aaaatgaatg attccactga
     2641 tgaaatgaaa agaaaatatg cagatcaaat tacagaatta aaacaaaaag aagaactaat
     2701 tcaaaaagaa gtagataaaa aaattgcaga gcttggaaaa aaaatgagcg aaagtgccga
     2761 tgaattgaaa acaaaatatg aagagcaaat taaagaatta aaaaagatga aagatgattt
     2821 actttataaa atgaataatt ggagtgaagt aacagaaaag aattttcgag ccttaacaga
     2881 agtaaatgaa aaaactttta aagaaatatc gcaaacttta aaacaaagtt tttcagaaat
     2941 aaaaaatctt tt
//
LOCUS       DAOSAS010000169         2889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_22290_length_2889_cov_15.930134, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000169 DAOSAS010000000
VERSION     DAOSAS010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22290_length_2889_cov_15.930134"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..708
                     /locus_tag="PLR72_06940"
     CDS             163..708
                     /locus_tag="PLR72_06940"
                     /inference="COORDINATES: protein motif:HMM:NF027435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4827 family protein"
                     /protein_id="HOK36970.1"
                     /translation="MKKAVNFFLCLMSLFLLFSSCSDRKTYAELLKDEKKIIADYIER
                     NNIQVVTTFPKINEWGEKVYVKTASGLYFHLVNPGDTSANADTVAQNDLVIVRYIQYT
                     LTVPADTISNWNTIDYPHPFTFNYLDYSQACQAFHEAVSYMKRNDSEAKIIVPSKIGF
                     SEYMNSVTPLGYDLKIKIQKY"
     gene            750..2153
                     /gene="glmM"
                     /locus_tag="PLR72_06945"
     CDS             750..2153
                     /gene="glmM"
                     /locus_tag="PLR72_06945"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOK36971.1"
                     /translation="MPLIKSISGIRGTIGGRAGEGLTPVDIVRFTAAYATFIKKSQCE
                     NFSKKIIVGRDARISGEMVYHLVAGTLAGQGLDVVNIGLATTPTTELAVIQEKALGGI
                     ILTASHNPMEWNALKLLTSKGEFLSAAEGEEVLKIAANENFDFATVEYLGKISDDFTF
                     LQKHIDNILSLDLVDVDAIKKADFTVAIDAVNSVGGIAVPALLKALGVKKIIKLNCEP
                     TGIFAHNPEPLPEHLSEISSLVKETKADVGFVVDPDVDRLAIVCEDGSMFGEEYTLVA
                     VADYVLQNKPGNTVSNLSSSRALRDITEKYGQHYSASAVGEVNVVALMKQTNAVIGGE
                     GNGGVIYPASHYGRDALVGIALFLSYLAKSKKTVSALKNDYPQYYISKNKIELTPSID
                     VDKILATIKNNYQRQYEVNDIDGVKIDFPTGWVHLRKSNTEPIIRIYAEAKTQEQAEA
                     FAKAIMEEVKRIAFDEK"
BASE COUNT          914 a          455 c          553 g          967 t
ORIGIN      
        1 ttttaaaata tttttttgtc tttaactttt attttgaaaa tacaagctac aaatttattt
       61 gtatttttca ttaaacaaag aataggagag atgttatttg attttcttaa cttgtgattt
      121 tcaaaattta attggatatt tgcgtaaaat ttgattcggg ttatgaaaaa agctgtaaat
      181 ttctttcttt gtttaatgtc cttgtttctg cttttctctt cttgcagtga tagaaaaact
      241 tacgctgagc ttttaaaaga tgagaaaaaa attatcgctg attatattga acgaaataat
      301 atccaagtgg tcactacttt ccctaaaata aacgaatggg gagaaaaagt ctatgtaaaa
      361 actgctagtg gcttgtattt tcacctggtt aatccgggtg atacttctgc aaatgctgat
      421 acggtagcac aaaacgattt ggtaatcgtt cgctatattc aatatacttt aactgtacct
      481 gctgatacta tttcaaactg gaacactatt gattatccac atccttttac ttttaattat
      541 ttggattatt ctcaagcatg tcaagctttt cacgaagcgg tctcctatat gaaacgaaat
      601 gattcagaag caaaaatcat tgtgccttct aaaattggct ttagtgaata tatgaatagt
      661 gttactccat taggttatga tttaaaaatc aagattcaaa agtattgaga acagaaatta
      721 atcagtgagt ttttataatt ttttttatta tgccgttaat taaatcaatt tcaggaattc
      781 gtggaacaat aggtggtaga gcaggagagg gcttaacacc ggttgatatt gttcgcttta
      841 ctgctgctta tgctactttc ataaagaaga gtcagtgtga aaatttttca aagaaaatta
      901 tagttggacg cgatgctcgc atctcaggtg agatggttta tcatttggtg gctggaacat
      961 tggctggtca aggtctggat gtcgtaaata ttggattggc aacaactcct accactgagc
     1021 tggcagtaat acaggaaaaa gctttgggtg gaataattct tactgcaagt cacaatccga
     1081 tggaatggaa tgctttgaaa cttcttacca gcaaaggtga atttttaagt gctgctgaag
     1141 gagaggaagt attaaaaatt gctgctaacg aaaattttga ttttgccact gttgaatatt
     1201 tggggaaaat ttctgatgat tttacttttt tgcagaaaca tattgataat atcctgtcac
     1261 tggatttagt agatgttgat gctataaaaa aagctgattt tactgttgct atagatgcag
     1321 taaattcggt gggagggata gcagtgccag ctttactgaa agctttggga gtaaaaaaaa
     1381 ttatcaagct caattgtgaa ccgacaggga tatttgcaca caatcccgaa ccgcttcccg
     1441 aacatttgtc tgaaatctcc tcgctggtaa aagaaaccaa agctgacgta ggttttgtag
     1501 ttgatccaga tgtagaccga ctggctatcg tttgcgaaga cggcagcatg tttggtgaag
     1561 aatatacgct ggtggcagta gctgattatg tcctccaaaa caagcctgga aacaccgttt
     1621 ctaatctcag ttcttcaaga gctttacgag atatcactga aaaatatggt cagcattatt
     1681 cagcttcggc agttggagaa gtaaatgtgg ttgccttaat gaaacaaaca aatgctgtaa
     1741 ttggcggtga aggtaatggc ggagttattt atcctgcaag ccattatggc cgcgatgcgt
     1801 tggtaggtat cgcacttttt ctttcttatc tggcaaaatc gaaaaaaact gtgtctgcat
     1861 tgaaaaatga ttatcctcaa tattatattt caaaaaataa aattgaattg actccgtcta
     1921 ttgatgtcga taaaattctt gcaaccatta aaaacaatta tcaacggcag tacgaagtta
     1981 atgatattga tggcgtaaaa attgattttc ctaccggttg ggtacattta cgtaaatcga
     2041 atactgagcc gattatccga atttatgctg aagcaaaaac acaagaacaa gctgaagcat
     2101 ttgccaaagc cattatggaa gaagtaaaaa gaattgcttt tgatgaaaag taaataattt
     2161 aatggatttt tgttttgcag tagttagttt gctggtagtt ttttggaaaa cccttctgac
     2221 ttcgacaatg cgtcacctat ttcgatgatg caaaaacaca aatatctcat tatttgactg
     2281 ttatagaata cctgccagaa tatgtcgaag tcaggagact gtttgaggtg taaaaattgt
     2341 ggttattctc gatgtgatta gttttatttg aaaagggccg tttcgaaaaa attgaaacag
     2401 ctcttatgtt aagtcttatt cagaaataaa aatttaatcg taagaattcc ccgatgcttg
     2461 cgtcggggta acttttgttt ttattttttt agaaattccg gtggcgaagc cataaaattg
     2521 gcgtaatacc tcgctgcttg cggcgaggat agtcaatttt aaggaatttc tttatttatt
     2581 agaaaaatac cgtcatttgc catgcttaaa aaaattagat gatttttagt actttaaaga
     2641 gtttacattt aaatgagcga tagtgttcct aataacactt cttggataaa aaaattcacg
     2701 caagcccaaa attaaaacta tcaaattcgt acagatgttt tatgaacctc tctacgtata
     2761 actggtatca taaacctcga atttcctacc ttgctggaat gtatcacggt ttgccatttc
     2821 ttgccttttt ccaactgcaa tatttcaact gccatttttc cagcttgaag agcactcttt
     2881 ccatcaacc
//
LOCUS       DAOSAS010000170         2875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_22438_length_2875_cov_12.884043, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000170 DAOSAS010000000
VERSION     DAOSAS010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22438_length_2875_cov_12.884043"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..>2875
                     /locus_tag="PLR72_06950"
     CDS             134..>2875
                     /locus_tag="PLR72_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOK36972.1"
                     /translation="MKKIFNLIFALFFPILFIQAQTSSGNGMITGKLIDKETKQPVAM
                     ANIRVLQQKDSTFVNGQASRNDGSFSIPVKYGAYIVHISFLGYKDIYKNVQVTSSRPI
                     VQLDTLYFEQTDITLSEIVIVERAPEIKVKGDTLEFNAGSFKVTESAVVEDLLKKMPG
                     VEIDKDGKITVNGREIKKILIDGEEFFSNDPKVASKNLPAKMVEKVQVLDKKSEMAEM
                     TGFDDSDEETVINLIIKQGMKEGLMGNAFAGYGSKDRYEGNAMVNYMKNKNQYTFIGG
                     LNNTNNAGFTDLASSMFGNIGGRGLRGMFGNQDGITTSGMGGFNFNTQLSKTFKLGGN
                     IRYGSTDNNVLSKVHTQNLIKDNNTFENENDTTNNYSQNFNMDLRMEWTPDTLTRIIF
                     RPSAGFYQNHRSETSDFFTTNELNDTINNGNSDYYSEGNGKNIGGRLDASRQLGKKGR
                     VLSLQLRGQLNDADNNGNNLSNTFYHGTKPDDIIDQRFTNTNDSKSWQGYISYVEPIG
                     KNNFIQLAYTYKQNLSESNKDTRTKDANGNYTVMDTTYSKRLENNFTNQSVELNFKAE
                     RGKYDYMVGFSMQPFNSNSKTYKGDSIYPVINQGINYAPTAQFNYRWSRQNNLRIRYS
                     GMTSQPSVTQLSPVMDVSDPLNVRYGNPDLKPSFSHRLNIRYMNFIPEKKSSITFFGN
                     AAYTLNDIVSSTITDITTGRKETTYKNVNGNWHANGRLTLNLPIRKTNFSISSMTFGS
                     YNHSKGFSNNEINLSQRTGLGETLGVDFRSSIIDFGIRGNVNYNNVQNSLKGQQNQEY
                     FNYNASANTTLYLPHDFSIDSDISYNTNSGYAAGFEQNAWLWNASIQKQLFKQKNGTL
                     RFKIYDILQQKSNINRSATSNYIRDITTNTLTSYFMVHFVYRFNIFKNGANQNDMMPR
                     "
BASE COUNT         1033 a          466 c          513 g          863 t
ORIGIN      
        1 attaaaaaaa gatttgtcat tgcaaaaatc ctattggtcg agaaaaaaac ttcatttgtc
       61 tattttattt gtgaatgtag aaaaacagaa ataattttga aacaaaaaag ttgaaatgat
      121 taacaagcaa aaaatgaaaa aaatatttaa tcttatcttt gccctatttt tccccatttt
      181 gtttatccaa gcacaaacat cctctgggaa tggtatgatt acagggaaac tgattgacaa
      241 agaaactaaa caaccagtgg ctatggccaa tataagggtt cttcagcaaa aagacagtac
      301 ttttgtcaat ggacaggcaa gtagaaacga tggttctttt tccatccctg tcaaatatgg
      361 tgcctatatt gttcatattt catttttagg atacaaagat atttataaaa acgttcaagt
      421 tacctcttcg cgtcctattg ttcaattaga taccctttat tttgaacaga ctgatattac
      481 cctttccgaa atagttattg tggaaagagc ccccgaaata aaagtcaaag gtgatacact
      541 tgaatttaat gctggctcgt tcaaagtaac tgaaagcgca gtagtagaag atctcttaaa
      601 aaaaatgcct ggtgtagaaa tagataaaga cgggaaaatt acagtaaacg gtcgtgaaat
      661 taaaaaaata ttaatcgatg gcgaagagtt tttcagtaat gatccaaaag tagcttctaa
      721 aaaccttcct gcgaaaatgg tagaaaaagt gcaggtatta gataaaaaat ctgaaatggc
      781 agaaatgact ggatttgatg atagtgacga agaaactgtt attaatctta taatcaagca
      841 aggaatgaaa gaaggactaa tggggaatgc ttttgcaggc tatggaagca aagaccgtta
      901 tgaaggaaat gctatggtaa attatatgaa aaataaaaat caatatactt ttattggtgg
      961 attgaacaat acgaataatg caggatttac tgatttggct tcgagcatgt ttggaaacat
     1021 aggaggacgc ggtttaagag gaatgtttgg gaatcaggat ggaattacaa cgtcgggtat
     1081 gggtggattt aatttcaata cacagctttc caaaactttc aaacttggtg gaaatatccg
     1141 ttatggctct accgataata atgttttgtc aaaagttcat acccagaatt taataaaaga
     1201 caacaatacg tttgaaaatg agaatgatac taccaacaat tacagccaaa atttcaatat
     1261 ggatttgcgg atggaatgga cacccgatac acttacacgt atcatttttc gtcccagtgc
     1321 aggtttttac cagaaccatc gtagcgaaac aagtgatttt ttcactacta atgaattgaa
     1381 tgatactatc aataatggta attcggatta ttattcagaa ggaaatggca aaaacatcgg
     1441 tggtaggttg gatgccagcc gtcaattggg gaaaaaggga cgtgttctca gcctccaatt
     1501 aagagggcaa ttaaatgatg ctgataataa tggcaataat ctttccaata ctttttatca
     1561 tggaacaaaa cctgacgata ttattgatca gagatttacc aacactaatg atagcaaaag
     1621 ctggcaagga tatatttctt acgtagaacc tattggaaaa aataatttta tccagcttgc
     1681 ctatacttat aagcaaaatc tttccgaatc gaacaaagac acgcgtacca aagatgctaa
     1741 tggaaattat acggtaatgg atacaactta cagtaagcga ttagaaaata atttcacgaa
     1801 tcaatcagtg gaattaaatt tcaaagccga aagaggaaaa tatgattata tggttggttt
     1861 cagcatgcaa ccatttaatt ctaacagcaa aacttacaaa ggggattcaa tttatccggt
     1921 tatcaatcaa ggaattaatt atgctcctac cgctcagttc aactatcgct ggagccgaca
     1981 aaataattta agaatcaggt attcgggtat gacaagtcag ccttctgtta cccaactttc
     2041 gcctgttatg gacgtttccg atccattgaa tgtaaggtat ggtaatcctg atttaaagcc
     2101 ttctttcagt catcgactta atattcgcta tatgaatttt attcctgaaa agaaaagttc
     2161 cattactttt tttggtaatg cagcctacac cttaaatgat atagtttctt caacaattac
     2221 tgacatcaca actggtcgaa aagaaactac ttataaaaac gtgaacggca actggcatgc
     2281 taatggcaga ttgacgctga atttgccaat aagaaaaact aatttctcta tttcttccat
     2341 gacttttggc agttacaatc attcaaaagg tttttctaat aatgaaatca atttaagcca
     2401 acgaacagga ttgggagaaa ctttgggtgt agattttcgt tccagtataa tagattttgg
     2461 cattcgtggg aacgtaaatt acaacaatgt tcaaaatagt ttgaaagggc agcaaaatca
     2521 agaatatttt aattataatg cctctgccaa caccaccctt tatttacctc atgatttttc
     2581 tattgatagt gatatttcct acaataccaa ttcaggttat gcggcaggtt ttgaacaaaa
     2641 tgcgtggctt tggaatgctt ctattcaaaa acaacttttc aagcaaaaaa atggtaccct
     2701 tcgttttaaa atatatgata ttttacaaca aaagagtaat atcaatcgaa gtgctacgtc
     2761 caattatatt agagatataa ctaccaatac acttaccagc tattttatgg ttcatttcgt
     2821 ataccgcttc aatattttca aaaacggtgc aaatcaaaac gatatgatgc cacgt
//
LOCUS       DAOSAS010000171         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_22605_length_2859_cov_11.504280, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000171 DAOSAS010000000
VERSION     DAOSAS010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22605_length_2859_cov_11.504280"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..761
                     /locus_tag="PLR72_06955"
     CDS             63..761
                     /locus_tag="PLR72_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOK36973.1"
                     /translation="MKKKILLTLLSTFVIFISCKPVEKKQNKKIDSTLQPMELTTFDT
                     IHLLQPDTTGGKPLMYALQHRKSDREFEQKNLSLKHLSEILWAAYGVNRPNGKRTVPS
                     AMALYPLQVYAVLSNGIYFYEPNQHALIPTVKGDYRSLAGSQDFVYTAPLNLIFIADY
                     HKYNTDRKIPDENRIRLVSLDAAHSTQNVYLYCASEGLKCVVRAGAKEKELLEVLKLD
                     PNYYQFIVAQTVGY"
     gene            complement(853..2175)
                     /locus_tag="PLR72_06960"
     CDS             complement(853..2175)
                     /locus_tag="PLR72_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOK36974.1"
                     /translation="MNWEQLLSTKRFGLESYHEPRKDSRTEFQRDYDRLIFSPPFRRL
                     QNKTQVFPLPGSIFVHNRLTHSLEVSSVGRSLGINIARCLADCHPNCPIIHEIDAIVA
                     AACLAHDMGNPPFGHSGEKAIATFFSEGEGQKLAEKMSPVEWKDLVCFEGNANTFRLL
                     THQFAGRRKGGFVLTYSTLASIVKYPYAAAYTNSQHSKFGFFNSEADDFKKIADELGI
                     INVESNSFHYVRYPLVFLIEAADDICYQLMDIEDAHKLKILSTEETKFLFLNYFDENQ
                     KRQKMEVLNLVNDENEQIAYLRSSVIGKLVEECSQIFVDNEENILNGTFHTSLIEQLP
                     PLSARAYQYCSDVANSKIYCSKQVLEVELSGYKIILTLLENLIRAVLQPQKAYSQQLL
                     MRIPSQYEINHPTMYGKIMAVLDYISGMTDVYALDFYKKINGIDLPTL"
     gene            complement(2182..>2859)
                     /locus_tag="PLR72_06965"
     CDS             complement(2182..>2859)
                     /locus_tag="PLR72_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOK36975.1"
                     /translation="RAKYINIKILTDGLRVTLPVNSTKNDALKFIDSIRSKIIKKQLL
                     IKQRNEKNVIVLNKNTVFSTLTFQIAIKEEKRKNIYFELRNGWLNIFVPENYNFQQPV
                     MQKHLWNGVFYFLRQEAKRFLPKRTQKLAEQHNFKYKQVKIQSSKTRWGSCSNHLNIN
                     LSFYLMLLPIHLVDYVILHELCHTVEMNHGTNFYQILERVTNGDSDKLKNELKKYSSM
                     KFPINIE"
BASE COUNT          984 a          499 c          424 g          952 t
ORIGIN      
        1 ttggctaaat ccaaaaaata aattaaattt gaaacatctt ttatcatttt aaattcaaac
       61 ctatgaagaa aaaaatatta cttactttat tgtcaacgtt tgttatcttt atttcgtgca
      121 aacctgttga aaaaaagcaa aataaaaaaa tcgattcaac tttacagcct atggaactta
      181 ccacttttga cactattcac ttgttgcaac ccgacacaac aggaggtaag ccattgatgt
      241 atgctttgca acatcgcaaa tcagatagag aatttgaaca aaaaaatctg tcattaaaac
      301 atctttcaga aattttatgg gcagcttatg gggttaatcg tcctaatggt aaacgaaccg
      361 taccttcagc tatggctctt tatccgttgc aagtttatgc tgttttgtca aatggtattt
      421 atttctatga acctaatcag catgcgttaa tacctacggt aaaaggtgat tatcgttcgc
      481 tggctggatc gcaggatttt gtttacactg caccacttaa tctgattttt attgccgatt
      541 accacaagta taatactgat agaaaaattc ctgatgaaaa ccgtattcga ttggtttcac
      601 tggatgcggc tcactctact caaaatgttt atttgtattg tgcttctgaa ggtctaaaat
      661 gcgtagttcg agcaggtgca aaagaaaaag aattactcga agtgttaaaa cttgatccaa
      721 actattatca atttattgtc gctcaaaccg ttggctatta atgattaaaa aaataaattt
      781 aacttcataa acattcactt attaaattga aacaagattg aaaaaaaacc aaaaaatcaa
      841 tcttgttcct ttttataaag tgggtaaatc aataccattt atttttttat aaaaatccag
      901 cgcatacaca tctgtcatcc ctgaaatgta atcaagaaca gccattattt taccatacat
      961 agtaggatga ttgatttcat actgagaagg aatacgcatt aaaagttgtt gagaataagc
     1021 cttttgaggt tggagaacag cacgtattaa gttttcaagc aaggttaaaa taattttata
     1081 ccccgatagt tccacttcca atacttgctt agagcaataa attttactgt ttgctacatc
     1141 cgaacaatat tgataagccc ttgcagataa aggtggcaat tgttcaataa gagaagtatg
     1201 aaatgtgcca tttaaaatat tttcttcgtt atcaacaaaa atttgtgaac attcttccac
     1261 cagtttccca atcacagaag aacgtaaata ggcaatttgc tcattttcat cattgaccaa
     1321 attcaacact tccatttttt gtcgtttctg attttcatca aaataattca aaaaaagaaa
     1381 tttggtttct tctgttgaaa gaatttttaa tttatgagca tcttcaatat ccattaattg
     1441 ataacaaata tcatcagcag cttctatcag aaaaactaaa ggataacgca catagtgaaa
     1501 agaattactt tcaacattta taattcctaa ttcatcagca atttttttaa aatcgtcagc
     1561 ttcggaatta aaaaaaccaa attttgagtg ttgactattt gtgtaagcag cagcataagg
     1621 atattttaca atagaagcaa gtgtactgta agtaagtaca aaaccacctt ttcgcctgcc
     1681 agcaaactga tgggtaagta atcggaaagt attggcattt ccttcaaagc aaaccaaatc
     1741 tttccactcc acaggagaca ttttttctgc caacttttgt ccttcccctt cagaaaaaaa
     1801 agtagcaata gctttttcgc ctgaatgacc gaaaggcgga ttgcccatat catgagccag
     1861 acaagcagca gcaacaattg catcaatttc atgaataatt ggacaattag gatgacaatc
     1921 agctaaacag cgagctatat ttatgcctaa tgaacgccct acactcgaca cttcgagact
     1981 atgagtcaaa cgattatgca caaaaatact tccaggcaaa gggaaaacct gtgttttatt
     2041 ctgtaatcga cgaaatggag gtgagaaaat caaacgatca taatcccgtt gaaattcagt
     2101 tctgctatcc tttctgggtt cgtgatacga ttctaatcca aaacgtttgg ttgataaaag
     2161 ttgctcccaa ttcatcataa gtcattcaat attaatagga aatttcatac ttgaatattt
     2221 ttttaactca tttttaagtt tatcgctatc cccattagta actctttcca atatttgata
     2281 gaaattagtt ccatggttca tttccactgt atgacatagt tcatgaagaa tcacataatc
     2341 caccagatga ataggcaaca acattaaata aaaggataaa ttaatattta aatgatttga
     2401 acaacttccc cagcgcgttt tactggattg aatttttact tgtttatatt taaagttatg
     2461 ttgttcggct aatttttgcg tacgttttgg caaaaaacgt tttgcctctt gacgcaaaaa
     2521 ataaaaaaca ccgttccaca aatgtttttg cataactggt tgctgaaaat tataattctc
     2581 tggtacgaaa atgtttaacc aaccatttct taattcaaaa tatatatttt ttcttttttc
     2641 ctctttgatg gcgatctgaa aagtaagtgt agagaaaaca gtatttttat tcaatacaat
     2701 cacatttttt tcattccttt gctttatcaa caattgcttt ttgataattt ttgaacgaat
     2761 agaatcaata aatttcaagg catcattttt tgttgaatta acaggtaaag taacgcgcaa
     2821 gccatcagtt aaaattttaa tatttatgta ttttgctct
//
LOCUS       DAOSAS010000172         2853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_22676_length_2853_cov_12.229807, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000172 DAOSAS010000000
VERSION     DAOSAS010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22676_length_2853_cov_12.229807"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..707)
                     /locus_tag="PLR72_06970"
     CDS             complement(27..707)
                     /locus_tag="PLR72_06970"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOK36976.1"
                     /translation="MGIAGNQIEQTVEEILQKIKKSMNGVIASQMNKNGIIYQKNYGV
                     TIPRLKEIAAQYESQHELAQKLWEFPIRETKILATLLEPAEKFSYSMAQQWMEECTQI
                     ELIEQTCMNLFSRLSYASQLCCEWISSENDWKKIAGFILSSRISNTLTSEEKNFITEK
                     AFENSTTENYHLYKAISICLSRFCHTSKEIAQEILQQIESFFDPLSTSQHYIKEAVEQ
                     ELNFLELL"
     gene            814..889
                     /locus_tag="PLR72_06975"
     tRNA            814..889
                     /locus_tag="PLR72_06975"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:849..851,aa:His,seq:gtg)
     gene            complement(1005..2369)
                     /gene="radA"
                     /locus_tag="PLR72_06980"
     CDS             complement(1005..2369)
                     /gene="radA"
                     /locus_tag="PLR72_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOK36977.1"
                     /translation="MAKSKTVFVCQNCGVESPKWIGKCPQCGEWNTYVEETVRKTESS
                     SSTNNHLPHQKPVLLSEVEATEEIRIDTLNKELNRVLGGGLVPGSLVLIGGEPGIGKS
                     TLVLQIILHLKNKKTLYVSGEESIQQIKLRAERLQLHHPSCYLASETSLTQIFDDIQE
                     IQPDLVVIDSIQTISSDTVESYAGSVSQVRECALAFLKFCKSTSTPVILIGHINKEGN
                     IAGPKVLEHIVDTVLQFEGDRHYMYRILRPIKNRFGDTSELGIFEMLHNGLREVSNPS
                     ELLLSQNHEGLNGIAIASAMEGIRPFLIETQALVSSAAYGMPQRSCTGFDIRRLNMLL
                     AVLEKRVGFKLSQKDVFLNIAGGLKVNDPAIDLAIISAILSSNIDVAIERHVCMAGEV
                     GLSGEIRPINRIQQRIHEAEKLGFTKMIVPYSNLKKNEIEKIKIEIIQVKKVEEAFKV
                     LFKK"
BASE COUNT          891 a          496 c          464 g         1002 t
ORIGIN      
        1 aaaggtagta tttttttctg tgttttttat aatagttcca gaaaatttaa ttcttgctca
       61 actgcttctt ttatgtaatg ttgactggta gaaagcggat cgaaaaatga ttcaatttgt
      121 tgcaaaattt cttgagctat ttctttactt gtatgacaaa atctacttaa acaaatgcta
      181 atggctttgt ataaatgata attctctgtg gttgaatttt caaaagcttt ctcagtaatg
      241 aaattttttt cttcactggt tagcgtattg cttatccttg aagaaagtat aaaacctgct
      301 attttcttcc aatcattttc ggacgagatc cattcgcaac aaagttggga agcatacgat
      361 aaacgcgaaa ataaattcat acaggtttgt tcaattaatt ctatttgagt acattcttcc
      421 atccattgtt gtgccatcga ataactgaat ttttctgcag gttccaataa agttgctaaa
      481 atctttgttt cgcggatagg aaattcccaa agtttttgag ctaattcgtg ttgcgattca
      541 tactgtgcag caatttcttt cagtcgagga atggtaacac cataattttt ttggtaaata
      601 atgccatttt tattcatttg agaagcgata acaccattca ttgatttttt tattttttgt
      661 aagatttcct caaccgtttg ttcaatttga tttccagcga ttcccattat ttttttacat
      721 tttagtgata aaagaaataa tcgcaaaaag tttttccttt taaaaatagt tgcataaaag
      781 caaatactat attatctttg cactaccaaa aatggtggtt gtagctcagt tggttagagc
      841 gtcggattgt ggttccgaat gtcgcgggtt caagtcccgt cctccacccg aagaggcagc
      901 gaaaagctgc ttttttgttt ttgtaaatat gaagtggcac ctaaaacact taccataaaa
      961 atcaaaaagt tctgattgtt attttgtgtc tacggagaag aaatttactt tttaaacaac
     1021 actttgaaag cttcttctac tttttttacc tgaataattt ctatttttat tttttctatt
     1081 tcgttttttt tcaagttaga ataaggaaca atcattttgg taaatcctaa tttttctgct
     1141 tcatggatgc gttgctgaat tctatttatt ggacggattt cgcctgaaag ccccacttcg
     1201 ccagccatac aaacatgtct ttcaattgcc acatcaatat tggaagaaag aatagctgaa
     1261 attatagcta aatcaattgc aggatcatta acttttaaac cacccgcaat attcagaaaa
     1321 acatcttttt gtgataattt aaaacctacc cgtttttcca gcactgccag caacatattc
     1381 aaccgacgta tatcaaaacc tgtgcaggaa cgttgaggca tgccataagc agcagaactc
     1441 actaacgcct gtgtttcgat gaggaaagga cggattcctt ccattgctga tgcaatagcg
     1501 ataccgttta gtccttcgtg gttttgcgaa agtaataatt cagaaggatt gctaacttcg
     1561 cgaagaccat tgtgcaacat ttcaaaaatt cccaattccg aagtatcacc aaaacgattc
     1621 tttataggac gcaaaattcg gtacatgtaa tgtcggtcgc cttcaaattg cagaacagta
     1681 tctacaatat gttccagcac tttcggaccc gcaatatttc cttctttatt aatatgtcca
     1741 attagtatca caggcgtgga agtagattta caaaatttaa gaaaagctaa agcacactct
     1801 ctgacttgcg atacagaacc tgcataagat tctacggtat cactcgaaat agtttggata
     1861 gaatcaatca ctaccaaatc aggttgaatt tcttgaatgt catcaaagat ttgcgttaac
     1921 gatgtctcgc ttgccaaata acacgaagga tgatgaagtt gaagcctctc tgcacgaagt
     1981 tttatttgtt gaatgctttc ttcgcctgag acataaagag tttttttgtt ttttagatga
     2041 agaatgattt gtaaaacaag tgttgatttt cctattcccg gttctccgcc aatcaatacc
     2101 aacgagccag gaaccaatcc gcctcctaaa acccgattca attctttgtt tagcgtatca
     2161 attcgtatct cttcggtggc ttctacctcg gaaagtaata caggtttttg atgaggcaaa
     2221 tgattgtttg tagatgaaga tgactctgtt tttctaaccg tttcttctac ataagtattc
     2281 cattcaccac actggggaca tttaccaatc cacttaggag attctacccc acaattttga
     2341 caaacaaaaa ccgttttact ttttgccatg aatttataat aatcttttaa aaatcaacaa
     2401 tttgctaaaa taaaaataat aaatcatttt tacaaaagta gtgtttttta ctggacggaa
     2461 agtaaacttt ttgaaaatct tttttgtgtc ttgctaagtt ggatttaaat atactcgaaa
     2521 tttttcgttc gacatagcct actggctgcg tcacactaaa ataaaattta ttgattttga
     2581 aaataaggta ataaggtggt tgtaagctgt aggaaacatt ccctgttatg aaggttaatg
     2641 gtttatgata aggtttttta atataaacaa ttttctgatg cgaaataatt tttggatggt
     2701 acaacggaat ttttctccgt gaatcgaaaa taattatctt tgtaaaataa ataaaaaagt
     2761 attgttgaat gttatatttc aatcaagaaa aaaccataaa aatatgattc ttttttttca
     2821 agcttctgat aatcaaatat atgcagttca atc
//
LOCUS       DAOSAS010000173         2838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_22864_length_2838_cov_12.473590, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000173 DAOSAS010000000
VERSION     DAOSAS010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22864_length_2838_cov_12.473590"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..649
                     /gene="rpe"
                     /locus_tag="PLR72_06985"
     CDS             8..649
                     /gene="rpe"
                     /locus_tag="PLR72_06985"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013671217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOK36978.1"
                     /translation="MSPSILTADFSNLQAICELINQSEADWIHLDVMDGVFVPNISFG
                     FPVIKAVKKHCKKPLDVHIMIIHPEKYVQRFAEAGAGILTFHYEAAEHPAEIIEQIHA
                     AGMKAGITINPDIPVKVLEPFIEKVDMVLLMSVFAGYGGQKFIEETYQRINELKSIIQ
                     QKNPSCLLEVDGGINLENAPKLFAQGVDVLVVGNTVFNSNQPLEIIHQLKTIA"
     gene            676..2730
                     /locus_tag="PLR72_06990"
     CDS             676..2730
                     /locus_tag="PLR72_06990"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOK36979.1"
                     /translation="MDFLYRTPFVRLLFTLIIGILIYQYIELYTFTRYAIYLIATFLI
                     ISPFFIQDAKNQFHFRWLFGVGTFLIFAMLGYSLSAQHEKSTSFSPLNKKGIYIVELT
                     SAPTEKAKSFFYSTRVKQFLNDDRWETAHGKAYLYLQKDSAASSLIVGDQLLIETEFK
                     APEKALNPDEFDYATYLKRQGVGATAYVPTGYWEKAGENHSFSLIRFSDQCRNYLFKI
                     YQKFNIHGDEFSVLAALTLGYKDDLNPDLKKSYSASGAMHILSVSGLHVGTVYAVLLF
                     LFGFLQNKKPQKLLKSILLILSLWGYAFITGLSPSVVRATSMFTFVAVATCLDRKSEI
                     YNTICMSAFFMLLVNPNYLFDIGFQLSYAAVFSIIYFQPYFTIFIPVSNKILKSLRDL
                     LTVSVAAQIGTAPFTLYYFQQFPNYFLFTNLIVIPLSTIIIYLAVGLFAISFVPYVSN
                     LVAFLLNISVKLLNFCIVKIEHFPYSISQVSLDSKQSFFIILFIFCISAYFYYKKYTP
                     LIMGLLSILFIISIDFYARYQTLNTRRMIVYAGQRNTHVNFIDQGNNFLFSTDEKEAE
                     RVATSYWRKHKLKPPIEVQNTSWFTNGFACFQGQRIFILINDELKNKIAQKPLEVDYL
                     IIGNGQKIRMEQLLSCIHPRKVIVDKTISKWYADNIQQTCEKQKIEFYSIANEGAFIL
                     NF"
BASE COUNT          872 a          459 c          470 g         1037 t
ORIGIN      
        1 aaatcttatt tctccttcaa tattaacagc agattttagt aatctgcaag caatatgcga
       61 acttattaac cagagcgaag cggattggat tcatctggat gtgatggatg gcgtatttgt
      121 acccaacatt tcttttggtt ttcctgtaat taaagctgta aaaaaacatt gtaaaaaacc
      181 actggatgtc cacattatga ttattcatcc tgaaaaatat gtgcaacgtt ttgcagaagc
      241 aggagctggg atacttactt ttcattatga agcagcagaa caccctgctg aaattattga
      301 acaaattcac gcagcaggaa tgaaagcagg aattaccatc aatcctgata tcccggtaaa
      361 agtgctggaa ccttttatag agaaagtaga tatggtgttg ttaatgagtg tttttgctgg
      421 ttatggaggt caaaaattca ttgaagaaac ttatcaacga atcaatgaat taaagtcaat
      481 aatccagcaa aaaaatcctt cttgtttgct ggaagtagat ggtggcataa atttggaaaa
      541 tgctccaaaa ctttttgctc aaggagtgga tgtgttggtg gttgggaata ctgtttttaa
      601 ctcaaatcag cctctggaaa ttatccacca attaaaaaca atagcttgat tttttcgatt
      661 tcattcctct gatgtatgga ttttttgtat cgtactcctt ttgtaagatt gttgtttact
      721 ctgataattg gaattcttat ctatcaatac attgaattat atacatttac aagatatgcc
      781 atttatttaa ttgctacttt tctgatcatt tccccttttt ttattcaaga tgcaaaaaat
      841 caatttcatt ttcgatggct atttggcgta ggtacttttt taatttttgc catgctgggc
      901 tattcattaa gtgcccaaca tgaaaaatca acttcatttt ctcccttaaa taaaaaagga
      961 atttatattg ttgaattaac ttcagctccc acagaaaaag caaaatcatt cttttattct
     1021 actcgagtaa aacagttttt aaacgatgat cgctgggaaa ctgctcacgg caaagcttat
     1081 ctgtatcttc agaaagatag tgcggcttcg tctctgattg ttggcgacca gttattgata
     1141 gagactgaat ttaaagctcc agaaaaagca ttgaaccctg acgaatttga ttatgcaact
     1201 tatttaaaaa ggcaaggcgt gggtgcaact gcttacgttc caactggcta ttgggaaaaa
     1261 gcaggtgaaa atcattcttt ctcgctcatt cgtttttcag atcaatgcag aaattatctg
     1321 tttaaaatct atcaaaaatt taatattcat ggtgatgaat tcagtgtgtt ggcagcattg
     1381 actttaggat acaaagacga cctaaatcct gacttgaaaa aaagttatag tgcgtcaggg
     1441 gcaatgcata tattatctgt aagtggtttg catgtgggaa cggtttatgc ggtattgctt
     1501 tttctgtttg gttttttaca aaataaaaag ccacaaaagt tgttaaaaag tatccttctc
     1561 attttatcat tgtggggata tgcttttatt acaggattat ctccttcagt ggttcgagct
     1621 acttcaatgt ttacttttgt agcagtagct acctgccttg atcgcaaatc tgaaatttac
     1681 aacactattt gtatgtcggc ttttttcatg ttgctggtga accccaatta tttatttgat
     1741 attggtttcc aacttagtta tgcagcggta ttcagtatta tctattttca accttatttt
     1801 actattttta ttcctgttag caataaaata ctgaaaagtt tgcgtgattt acttactgtt
     1861 tcagtagctg cacaaattgg caccgcacct tttacgttat actactttca acagtttcct
     1921 aattattttc tgtttaccaa tttaatagta attccactgt ccactattat tatttacctt
     1981 gctgttggac ttttcgctat ttcatttgtt ccttatgttt ctaatcttgt agctttttta
     2041 ttaaatattt ctgttaaact cctcaatttt tgcattgtaa aaattgaaca ttttccttat
     2101 tctatttctc aagtttcatt ggatagcaaa caaagttttt ttattattct tttcattttt
     2161 tgcatctcgg cttatttcta ttataaaaaa tatacgcctt taataatggg attgttgtct
     2221 atcttgttta ttatttctat cgatttttat gctcgatatc aaaccttaaa tacccgtcga
     2281 atgattgtat atgctggaca aagaaatact catgtaaatt ttattgatca aggaaataat
     2341 tttctttttt ctactgatga aaaagaagca gaacgtgtgg ctacttctta ttggagaaaa
     2401 cacaaactaa aacctcctat cgaagtacaa aatacttcct ggtttacaaa tggctttgcg
     2461 tgttttcagg ggcaacgcat atttattctt attaatgatg aattaaaaaa taaaatagcc
     2521 caaaaacctc tcgaagtaga ttatctaatt attgggaatg gacaaaaaat acgtatggaa
     2581 caacttttat cgtgcattca tcctcgcaaa gtaattgtag ataaaaccat ttcaaaatgg
     2641 tatgctgata acattcagca aacttgtgaa aaacaaaaaa ttgaatttta ttctatagct
     2701 aacgaaggag cttttatttt aaatttttag tacttttcgg ggcattcccg tttttttaac
     2761 attcgtgaga tagtatgaga aattagctaa gttcaacata gaagtgcgaa tttagcagag
     2821 atgttttgca tgattttt
//
LOCUS       DAOSAS010000174         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_23275_length_2800_cov_10.059745, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000174 DAOSAS010000000
VERSION     DAOSAS010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23275_length_2800_cov_10.059745"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="PLR72_06995"
     CDS             <1..1575
                     /locus_tag="PLR72_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK36980.1"
                     /translation="ANIYASELKAEKTETDYLLKFTLNENAAAVRINIFDGENVVKTI
                     DAGTLNKGIQSVSVPLAELPTGFFTWSVTAMAEAVDRPVKISDDSRPELQFYSPRGVA
                     VDNNFESPFFGRVYASETVGGTVTNRTTQDGVYILNAALEDVTQQGASSYAGSVAWGG
                     SNSPMRLAVAEDGKVYICDWSDAHSGVWIMDPASPTSPFIPVFDGLTRDSKGLASYNG
                     VNVHGSISHCWVLGTGEDTRLYTFDEDYVDAVATKPGNLLQYNIGTLPTPWKQAPSAI
                     IYNNALNGNLQQNFNSCIAPDGRGGWWISQYRATDAANIPSLIHVNTEGLVDFNSGKT
                     PTLIENSYTGGMAVTVDGKTLAMGCNNEVKIFSVIFSDSGTPSLTRLYSIKPAMGTNT
                     AGLAFDRAGNVYVISNSSERLGVWSLPKIENVFTTPAPNSEKLDVISEVPQLEKEKTE
                     ILLYPNPVNNYLKIESKNETLKMVTIYDLRGSMVRSERISGISAEISTDNLPAGNYII
                     KVQTENRVITKRIIKQ"
     gene            1772..>2800
                     /locus_tag="PLR72_07000"
     CDS             1772..>2800
                     /locus_tag="PLR72_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36981.1"
                     /translation="MRKITLFMAALAFSFYAMADGMSGTYKVGTGETAPNFTLLSEAV
                     TAINTNGLTGDVILEITSDITETVNIGLINNSDFTITIRPDQDADRTITFDKATDNDG
                     PSGAFCLGIGMGLKWADLSPTKNVIIDGYAIGGNTRRLKITTAVTHNGLDGPILLMDD
                     CSNIQIKNLIIHHVGTSTGSSSNYGIYLRVNTSYGTKKMPSNVLIENNEITAIQNTAS
                     QGIGIYANAAPASLATGVMIKNNIIKAKTRGIFLYYTNDLNIINNEFRINQPSDGMLS
                     TAIMGNNGQTGNINVIGNKFIELKTGNSTAGAYGMRAIIASGGGTWNIINNFFTGFDK
                     TSTKAGETM"
BASE COUNT          911 a          448 c          574 g          867 t
ORIGIN      
        1 gccaatattt atgcttcaga attaaaagcc gaaaaaacag aaacagacta tctattgaaa
       61 tttacattga atgaaaatgc tgctgctgtg agaattaata tttttgatgg tgagaacgtt
      121 gttaaaacta ttgatgcggg aactttgaat aaaggaatac aatctgtatc ggttccctta
      181 gctgaattgc caacagggtt tttcacttgg agcgtgacgg caatggctga agctgttgat
      241 cgtcctgtaa aaatttctga tgactcgcgt cctgaacttc aattctattc accacgtggc
      301 gtggctgtcg ataataattt tgaaagtcct ttctttggac gggtgtatgc ttctgaaaca
      361 gttggaggaa cagtaaccaa tcggacaact caagatggtg tttatatttt aaatgcagca
      421 ctcgaagatg ttacccagca aggtgccagc agttatgccg gttcagtagc atggggaggg
      481 agcaatagtc cgatgcgact ggcagtagct gaagatggta aagtttatat ttgtgattgg
      541 agtgatgctc attcaggtgt ttggatcatg gaccctgctt cgcctacttc gccttttatt
      601 cctgtattcg atggattaac aagagatagt aaaggtttag cttcttataa tggcgttaat
      661 gtgcatggct ctatttctca ttgttgggtg ttaggaacag gagaagatac tcggttgtat
      721 acttttgacg aagattatgt ggatgcagta gctacaaaac cgggtaacct tcttcaatat
      781 aacattggaa ctttaccaac tccctggaag caagctccaa gtgccattat ttataacaat
      841 gccttgaatg gcaatttaca gcagaacttt aattcctgca ttgctcctga tggacgaggt
      901 ggctggtgga tttctcaata tcgggctacc gatgctgcaa atattccttc gttgattcat
      961 gtcaatactg agggattggt agattttaat tctggaaaaa caccgacgtt gattgaaaat
     1021 tcctatacag gtggaatggc agtaactgtt gatggaaaaa cgctggcaat gggttgcaat
     1081 aatgaagtga agatttttag tgtgattttc tccgacagtg gcactccttc tttaacccga
     1141 ctttattcca ttaagccggc tatgggaacc aataccgcag gtttggcttt cgaccgggct
     1201 ggtaatgtgt atgtcatctc caattcttcg gaaaggctgg gtgtatggtc attaccaaaa
     1261 atagagaatg tgtttactac accagctcct aacagcgaaa aattagatgt aatcagtgag
     1321 gtcccgcagc ttgaaaagga aaaaacagaa attttactgt atccaaatcc tgtgaacaac
     1381 tatttaaaaa ttgaaagtaa aaatgagact ttaaaaatgg ttaccatata tgatttgaga
     1441 ggtagcatgg tgcgttcaga aagaataagt ggcataagtg ctgaaatttc tactgacaat
     1501 ttgcctgcag gaaattatat tattaaagtg cagacagaga atagagtaat aaccaaacga
     1561 atcatcaaac aataaatttc tatatcttaa agacagcagt gtttacaggc attgctgtct
     1621 tttggatttt ttgctaaata aactatttta gtaataattt ttaaaaacgt aaaaaatatt
     1681 tagcaaacgt ttgcagtttt acaaaattaa ttttatattt atcgcgttaa ataattttat
     1741 ttttttattt actttttaaa actattttat catgagaaaa attacgttat ttatggccgc
     1801 actggcattc agtttttatg ccatggccga tggaatgtct ggtacttaca aggtaggtac
     1861 aggagaaact gctccgaatt ttactttact aagtgaagca gtaactgcaa ttaataccaa
     1921 tggcttaaca ggcgatgtta ttcttgaaat tacatcagat attactgaaa cagtaaacat
     1981 tggcttaatt aacaattcag attttacaat aactataaga ccggatcaag atgcggacag
     2041 aactattacc tttgacaaag ctacagataa tgatggacca tcaggggctt tttgtctcgg
     2101 cattgggatg ggattaaaat gggctgattt atcacctact aaaaatgtaa taatagatgg
     2161 ttacgctatt ggaggaaata ctcggaggtt aaagattacc accgctgtaa cacataatgg
     2221 tttggatgga cctattttat taatggatga ttgctcaaat atccaaatta aaaatttaat
     2281 tattcatcac gttggtacat caacaggaag tagtagtaat tatggtattt atcttagagt
     2341 aaacacgagc tatggtacta aaaaaatgcc atctaatgtt ctaattgaaa acaatgaaat
     2401 cacagccatt caaaatactg cctcccaagg aataggtatt tatgctaacg ctgctcctgc
     2461 ttcgcttgct acaggtgtta tgataaaaaa taatattatt aaagccaaaa cgaggggaat
     2521 atttttatat tatacaaatg atcttaatat cataaataat gaatttcgaa taaatcaacc
     2581 ttcagatgga atgctaagta ctgcaattat gggtaacaat ggtcaaactg gcaatataaa
     2641 tgtaataggg aataagttta ttgagcttaa aacaggaaat tcaaccgctg gtgcctatgg
     2701 tatgagagct attatcgcaa gtgggggagg aacttggaat attatcaata atttttttac
     2761 gggtttcgat aaaacttcaa caaaagcagg cgaaacgatg
//
LOCUS       DAOSAS010000175         2799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_23288_length_2799_cov_14.061589, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000175 DAOSAS010000000
VERSION     DAOSAS010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23288_length_2799_cov_14.061589"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            560..1123
                     /locus_tag="PLR72_07005"
     CDS             560..1123
                     /locus_tag="PLR72_07005"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOK36982.1"
                     /translation="MEEEIKKTVKILQEGGVILYPTDTIWGLGCDATNEKAVTRIYEI
                     KKRTDTKAMLVLVDDASKILNYVEEVPEMAWQLVELTTKPLTIIYPKAKNLPYNLVAE
                     DGSIGIRVSDEEFSKKLCAQFGKPIVSTSANISGKATPLNFSEISDEIKSAVDYIVKY
                     RQKETLEPKPSSIIKIEKGNIIQIIRQ"
     gene            1245..2603
                     /locus_tag="PLR72_07010"
     CDS             1245..2603
                     /locus_tag="PLR72_07010"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOK36983.1"
                     /translation="MLASLIVWILFMLFRKIVNDSAIFENVQIFFPNFNYFSSLVLFP
                     FCCIIVHYLSGFYLYPEKMTGIKIFFSTFAASIIISITIFFVLLLDDIVVSYKYYYYS
                     LLVLFGLLFVFTYFPRLMIYLNVQKNYKTKRWTIPTLIIGTGQNAKKLADELVKRNQK
                     NTLVGFINVDHEVSVPKEKILGNLRNIRNIIEENHIQETIIALENASEQQLFSIINLL
                     YQYNVDIKFTPRLYEILTGSAKIKDLNVSPLVNITDINMPDWEQSVKRVFDIVFSLFS
                     LIILFPVMLFFMIWIKLDSKGPIFYLQERIGRYGRPFKIIKFRTMYEGAENGVPQLSN
                     PNDSRVTRVGKILRRYRIDEIPQFINVLLGDMSIVGPRPERRYYIQQIIQEAPYYCLL
                     YKIRPGLTSWGPIRIGYSDTIEKMIERLNYDIIYMDNMSLITDLRILLMTIKIIFKGS
                     GV"
BASE COUNT          937 a          438 c          443 g          981 t
ORIGIN      
        1 tgattttttc aaaaggagga aaaaatgaga ttttttgaag agaagtatta aattttttgt
       61 tgtttcctat attttacaat aatttgtaat ataaaattga gcattgataa tttctcctgt
      121 tcgagtatag gctacttatt aagttgtcga attttgtgac tcaattgaaa atccatatct
      181 tcctcgtgct tcttgcaacc cacttaaaat aaactctatg ttgtttccct ctccaaatcc
      241 ttgattttca aagctattat aagttccatt ggcataggct ttttctattg agtcagtgag
      301 tatctatcgg cttatttcaa cactttcttt gaaaaactca ttgtcaagcc gtaaaagcca
      361 cctcaacctg gtttaggata aagcatttaa tgactcagct attccattgt tttcaaaata
      421 aagaaagttg aaaatttctt catttcacgc atcacaactt tcttatcgac tgatttcaat
      481 aattcaatat ctttgcaaaa taaatagcct ccttctccgt tgtttaactt actactttat
      541 aaaaaacatg gcaaatagaa ttgaagagga gataaaaaaa actgtaaaga ttttacagga
      601 gggaggagtc atcttgtatc ctactgatac gatatgggga ttgggttgtg atgctactaa
      661 tgaaaaggca gtgacacgta tttatgaaat caaaaaaagg actgatacaa aagctatgct
      721 tgtacttgtt gatgatgctt caaaaatctt aaattatgtc gaggaagttc ctgaaatggc
      781 ttggcaactt gttgaattga ccactaaacc tcttaccatt atttatccga aggcaaaaaa
      841 cctgccatac aatcttgttg ctgaagatgg aagcattggt atcagagtct ccgatgaaga
      901 attttcaaaa aaattatgtg ctcaatttgg gaaacccatt gtctccactt cagccaacat
      961 tagtggtaaa gcaactcctt tgaatttttc tgaaattagt gacgaaataa aatcggctgt
     1021 agattatatt gttaaatacc gtcaaaaaga gactttagaa ccaaaaccct cttcaattat
     1081 taaaattgaa aaaggaaata ttattcaaat cattcgtcaa taattttttt gaattttttc
     1141 aagcaaacaa gctgtttttg aagtaaaaat aaaaaattat caactcatat tgattgaata
     1201 tcgtgaacaa aaagaagtta cttttaacct accttttttt tgatgtgctt gcttctttga
     1261 tagtatggat tttattcatg ctattccgaa aaattgtgaa tgattcagcg atatttgaaa
     1321 atgtccaaat ttttttccca aatttcaatt atttttctag cttagttttg ttcccttttt
     1381 gttgtattat agtgcactat ctatcaggat tttacctgta tcctgaaaaa atgactggta
     1441 ttaaaatctt tttttcaact tttgctgcct cgattattat ctctatcacc attttttttg
     1501 ttctactgct cgatgatatt gtggtttcct ataaatatta ctattattct ctgttagttt
     1561 tgttcggatt gctttttgtt tttacttatt tcccacgatt gatgatttac ttaaatgttc
     1621 agaaaaatta taaaacaaaa cgctggacta tccctacttt aataattggc acgggacaaa
     1681 atgcaaaaaa actggctgac gaattggtaa aaagaaatca aaaaaacact ttggtaggat
     1741 ttatcaatgt tgatcatgaa gtgagtgtac ctaaagaaaa aatattggga aatctccgaa
     1801 acattagaaa tattattgaa gaaaaccata tacaggaaac tatcattgct ctggaaaacg
     1861 caagtgaaca acaattgttt tccatcatta atcttttgta ccaatataat gttgatataa
     1921 aatttacgcc acgattatat gaaattctga ctggaagtgc aaaaataaag gacttaaacg
     1981 ttagtccgct ggtaaatatt acggatatta atatgccaga ttgggaacaa agtgtaaaaa
     2041 gagttttcga tattgtgttt tctctttttt cgctgattat tctttttcct gttatgcttt
     2101 tttttatgat atggataaaa cttgattcaa aaggtccgat attttactta caagaacgta
     2161 tagggagata cggacgacct ttcaaaatta ttaaattccg aactatgtac gaaggagcag
     2221 aaaatggtgt tccacaactg agtaatccga atgattcacg ggtaacgcga gtagggaaaa
     2281 ttttgcgtcg ttatcgtatt gatgaaatac ctcaatttat caatgttcta ttaggtgata
     2341 tgagtattgt cggtccaaga cctgaacgcc gttactacat tcagcaaatt attcaggaag
     2401 ccccttatta ttgtctttta tacaaaattc gccctggact tacttcctgg ggaccaattc
     2461 gcattggtta ttccgacacg atcgaaaaaa tgatcgaacg gttaaattat gatattattt
     2521 acatggataa catgtcactg attactgatt tacgaatatt actaatgact ataaaaatca
     2581 tttttaaagg atcaggtgtc tgatacaagt tgcgttttac aacggatctt ccttccagga
     2641 tcttgaaaag ctattataaa aatcgctacc aatggaggaa aatgtgattt ttagtttttc
     2701 ttttgtacgc aattatgtat agaaaggaaa attcattaaa aatagacaaa ttttaaacaa
     2761 tttaccacat aaattattaa caatcagctt attaaattg
//
LOCUS       DAOSAS010000176         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_23304_length_2797_cov_10.803793, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000176 DAOSAS010000000
VERSION     DAOSAS010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23304_length_2797_cov_10.803793"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..582
                     /gene="ilvN"
                     /locus_tag="PLR72_07015"
     CDS             28..582
                     /gene="ilvN"
                     /locus_tag="PLR72_07015"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOK36984.1"
                     /translation="MENTTLYTIIIFSENKVGVLNQITVIFTRRGLNIETLSVSPSAI
                     PGIHKFTITVFTTEEVVKKVVQQIDKRVDILKAYYNTDDDLVHQEIALYKVATDKLLA
                     RGSIEGLIRKYQIHILEITENCVVFEKTGHYEDTQKLFEELSQTIGVLQFIRSGRIAI
                     TKSKVERLSDMLEELKKLEEKIDK"
     gene            763..1812
                     /gene="ilvC"
                     /locus_tag="PLR72_07020"
     CDS             763..1812
                     /gene="ilvC"
                     /locus_tag="PLR72_07020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOK36985.1"
                     /translation="MAKINFGGVIEDVVTREEFPLSKALEVLKDETIAVIGYGVQGPA
                     QSMNLRDNGFNVIVGQAPEFKDDWDKAVNDGWVPGKTLFSVEEAASKGTIIQYLVSDA
                     AQRILWPTIKPHLTKGKALYFSHGFSITYKDYTGVVPPADIDVIMVAPKGSGTSVRRN
                     FLAGSGINSSFAVFQDATGKALERTLALGIGIGSGYLFPTTFEKEVFSDLTGERGVLM
                     GALAGIMDAQYEVLRKNGHSPSEAFNETVEELTQSLIRLVDEKGMDWMYANCSATAQR
                     GALDWRPKFKEAVMPIFEDLYQRVKDGRECKRVLESTGVKNYKELLEKELKEMRESEI
                     WAAGAEVRKLRPKTN"
     gene            complement(1960..>2797)
                     /gene="ispE"
                     /locus_tag="PLR72_07025"
     CDS             complement(1960..>2797)
                     /gene="ispE"
                     /locus_tag="PLR72_07025"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOK36986.1"
                     /translation="FCFKIKSMIVYPNAKINIGLNVIECLPDGYHSIETVFYPIGLYD
                     ALEIKPIENDKPVFSTSGLAIEGNPENNLVFKAYQLLHNSYHIPPVKIMLDKKIPSAA
                     GLGGGSADAAFTLKALNQLFNLNLSTQELESLAAKLGADCAFFIHNQPGFAIGKGNIF
                     KPISLNLKNFYLLLVKPPISVSTAEAYSLVKVRKPEISLEENIYKPITEWKNLIKNDF
                     EDLLFERYPILANIKENLYKMGALYASMSGSGSTIYGIFDEKPEKNKNFRNFFLSLIQ
                     LA"
BASE COUNT          963 a          401 c          503 g          930 t
ORIGIN      
        1 aaaaaattta ttaatcaaat tctcaacatg gaaaatacaa ccttatatac gattattatt
       61 ttttcagaaa ataaggtagg cgttttaaat cagattacag taatttttac acgacgaggc
      121 ttgaacatcg agactctatc agtatctcct tctgccatac caggaatcca taaatttact
      181 attactgttt ttactacgga agaagtggtt aaaaaagttg tccaacaaat tgataaaaga
      241 gttgatattt taaaagctta ttataatacc gatgatgatt tggttcatca agaaattgct
      301 ttgtacaaag tagcaacaga taaattgctg gcaaggggtt ccattgaagg tttgatacgt
      361 aaatatcaaa ttcatattct cgaaataact gaaaattgtg tggtttttga aaaaacggga
      421 cattatgaag atactcaaaa acttttcgaa gaattaagtc agacaattgg agtgttgcaa
      481 tttattcgtt cgggacgtat agctatcact aaatcaaaag tagaacgatt aagtgatatg
      541 ctcgaagaat taaaaaagtt agaagaaaaa atagataagt aatcggtttt ttaatcaata
      601 gaattcctcg agacttgcct cggggttatt tattaaaaaa taaatttttt atcagaaaaa
      661 attatttgtg taataacaat taagatacta attttaactt taaaataaaa gttttagaat
      721 aaacaatttt aatatttttt aatttttaaa aataaaataa agatggcaaa aattaatttt
      781 ggcggcgtta tcgaagatgt ggtaacccgc gaagagtttc cattaagcaa ggctttggaa
      841 gtgttaaaag atgaaactat tgcagtaata ggttatggtg tacagggtcc tgcacaatca
      901 atgaatttac gtgataatgg cttcaatgtt attgttggtc aggctccaga attcaaagat
      961 gactgggata aagctgttaa cgacggctgg gttccaggca aaactttatt ttcagtggaa
     1021 gaagctgcat ccaaaggcac tattattcaa tatttagttt ctgatgctgc tcaaagaatt
     1081 ttatggccaa ctattaagcc gcatcttacc aaaggaaaag ctttatattt ttcacatggt
     1141 ttttcaatta cttataaaga ttatactgga gttgtacctc ctgctgatat tgatgtaatt
     1201 atggttgctc ctaaaggttc tggaacgagc gtgcgtagaa atttcttagc tggttcaggc
     1261 atcaatagta gttttgctgt atttcaagat gcaacaggaa aagctttgga acgtacttta
     1321 gctcttggta ttggtatagg ctcaggatat cttttcccaa ctacttttga aaaagaagta
     1381 tttagtgatc ttactggtga aagaggcgtt ttgatgggtg ctcttgccgg aatcatggac
     1441 gctcaatatg aagtgctcag aaaaaatggt catagccctt cagaagcttt caacgaaaca
     1501 gttgaggagc ttacccaaag ccttatccgt ttggttgacg aaaaaggtat ggattggatg
     1561 tatgctaatt gctcagctac tgcacaacgt ggtgcacttg attggcgtcc aaaatttaaa
     1621 gaagctgtaa tgcctatttt tgaagatctt tatcaaagag taaaagatgg tagagaatgc
     1681 aaacgtgtat tggaatctac tggtgttaag aattataaag aattgcttga gaaggaattg
     1741 aaagaaatgc gcgaaagtga aatttgggct gcaggtgctg aagttagaaa attacgtcca
     1801 aaaactaact aattattttt atcatttttt catattttaa aagtcttgtt tcatcacatt
     1861 tgttgagaca agacttttta ttctttccca gctaaaactt gattccaaaa agttatattg
     1921 ttgattcact atacattata tgcaattaaa ttttatgcat tatgctaatt ggataaggga
     1981 aaggaaaaag tttctaaaat ttttattttt ttcgggtttt tcatcaaaaa ttccataaat
     2041 ggtcgatcct gaacctgaca tggaagcata aagagctccc attttgtata gattttcttt
     2101 gatatttgct aaaatgggat aacgttcaaa aagaagatct tcaaaatcat tttttattaa
     2161 atttttccac tctgtgattg gtttataaat attttcttcc agagaaattt ctggttttcg
     2221 cacttttacc agtgaataag cttcagcagt tgaaacagat atgggaggtt ttactaaaag
     2281 cagataaaaa ttttttaaat ttagagatat tggcttaaaa atgtttcctt tcccaatagc
     2341 aaagccaggc tgattatgaa taaaaaaggc acaatctgct cccaattttg cagctaacga
     2401 ctcaagttcc tgagtgctga ggtttaaatt aaataattga ttaagagctt ttaatgtaaa
     2461 agctgcatcg gctgagcctc caccaagtcc agcagcagaa ggaatttttt tgtccaacat
     2521 aattttaact ggaggaatat gataactatt atgtaacaat tggtaagctt taaaaaccaa
     2581 attgttctca ggatttcctt caattgctaa accagaagtt gaaaaaacag gtttatcatt
     2641 ttctattggt ttaatttcca atgcatcata aagccctatc ggataaaaca ctgtttcaat
     2701 agagtgataa ccatctggca aacattctat tacatttaat cctatattga ttttagcatt
     2761 gggataaaca atcattgatt taattttaaa acaaaaa
//
LOCUS       DAOSAS010000177         2760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_23747_length_2760_cov_6.302403, whole genome shotgun sequence.
ACCESSION   DAOSAS010000177 DAOSAS010000000
VERSION     DAOSAS010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23747_length_2760_cov_6.302403"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLR72_07030"
     CDS             <1..450
                     /locus_tag="PLR72_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36987.1"
                     /translation="FYGTREEKGLIEFIKETMGNLEIKYKEIYLLRNEEVYKIFDFEQ
                     GRGFMPDFILFLKTKDKKKVGVVETELYYQIFIEPKGNEFIGDDGTFKTGKEGWKERF
                     LEEITEKYGFEKVIKAENPNYRLIGLPFFNKDHNTNFKKEYELITKE"
     gene            716..1756
                     /locus_tag="PLR72_07035"
     CDS             716..1756
                     /locus_tag="PLR72_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36988.1"
                     /translation="MQDLFKHIAVKEKLDKYFLLVDESKGFRPAKQIIEEIASEFEDK
                     DGNFIQQFQTTGFDSRLWELFLFSLFNENGFEILNDYDRPDFHLKKSETELFVEASIS
                     NEKTDDIYTEEFIKEAIELKDINIQNELTDYYTIRLGSVLYSKLNKKYWELNWVKGKP
                     LILAITPFHNYIADFLPDSKIIEYLYGIAYKTEIKESRLELKEIKKLDTHRYKKKEIP
                     SNFFNQENVENISAVIFTNNCNVHKFNRMGFQKGYGDNDIIIVRSGLAFDSTPGSTGK
                     KFDTIVKQGEVNEDWKESVSIFHNPNAIHKIDKELFHGMRQLWLNNEGKFDGEIPNFF
                     VYNSLTISGINE"
     gene            2097..>2760
                     /locus_tag="PLR72_07040"
     CDS             2097..>2760
                     /locus_tag="PLR72_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36989.1"
                     /translation="MENMFMKFVNKNKWFVVFFLVWTFLHVIFLFNGNSEFYIEEFWP
                     FGDNGLRDYGWFEFFVYEALPLLIFVIVKLVGKDIKRKIKDIKRKIGDIYQFTILNHL
                     FLTAMNKTMQTIMPHVVAVILFLIISFWYFSPVLEGKQLIGHDTKSWMCMAKETIDYN
                     KTHKDKTLWTNSMFSGMPTYQISMTQPNNFSKYVDQFFRLFPAPVFYLILYLLGAYVL
                     LLA"
BASE COUNT         1044 a          376 c          479 g          861 t
ORIGIN      
        1 ttttatggca caagagaaga aaaaggattg attgaattta ttaaagaaac aatgggaaat
       61 cttgaaataa aatacaaaga aatttatctt ttgagaaacg aagaagtcta taaaattttt
      121 gattttgaac aaggaagagg ttttatgcct gactttattc tctttttgaa aacaaaagat
      181 aaaaagaaag ttggagttgt tgaaacagag ctttattatc aaatttttat tgagccaaaa
      241 ggaaatgagt ttatcggaga tgatggaaca ttcaaaactg gaaaagaagg atggaaagaa
      301 aggtttttgg aagaaattac tgaaaaatat ggatttgaaa aagttataaa agcggaaaat
      361 ccaaattata ggttgattgg tttacctttt ttcaataaag atcacaatac gaactttaaa
      421 aaagaatacg aactaataac aaaagaataa ggtgtttcga ttttttcaat tcacccccga
      481 cccctcttga aaaaaatcga aacggaaaaa attaaatttt cggaaataat atgaaaaaac
      541 tctctgctaa aaataacgag caaaaaaatg acttctccgc ttcgctacga cccttcggga
      601 cacacaacag ccgatatctg caagggcttc gccacattct cctccgctat cgctatggag
      661 aacgtcagat atcggcaaac gttatcggca atattaaaaa caaacaacac gaccaatgca
      721 ggatttattt aaacatattg ccgtaaaaga aaagcttgac aaatattttc ttttggttga
      781 tgaatctaaa ggatttcgtc ctgcaaagca gataattgaa gagattgcaa gtgaatttga
      841 agataaagat ggaaacttta ttcaacaatt tcaaacaact ggatttgatt cgagattatg
      901 ggaactgttt ttattctctt tgtttaacga aaatggattt gaaatactga atgattatga
      961 tagaccagat tttcatttaa aaaagagtga gactgaatta tttgttgaag ccagtatttc
     1021 gaatgaaaaa accgatgaca tctacacaga agaatttata aaagaagcga tagaacttaa
     1081 agatattaat atacaaaatg aattaactga ttattatact atccgattag gtagtgtatt
     1141 atattcaaaa ctaaataaaa aatattggga acttaattgg gttaaaggga aaccacttat
     1201 tctagcaatt actccatttc ataattacat tgcggatttt ttacctgatt caaaaatcat
     1261 agagtattta tacggcattg cttataaaac cgagattaaa gaaagtagat tagaattaaa
     1321 agaaataaaa aaactagata ctcatagata taagaaaaaa gaaattccat caaatttctt
     1381 caatcaggaa aatgtagaaa atattagtgc agttatattt acgaacaatt gtaatgttca
     1441 caaatttaat agaatgggat ttcaaaaggg ctatggtgac aatgatataa taatagttag
     1501 gtcgggttta gcatttgatt ctacgccagg gtcaactgga aaaaaatttg atacaatagt
     1561 aaaacaagga gaagtcaatg aagactggaa agaaagtgtt tcaatttttc ataatcctaa
     1621 tgctattcac aaaattgata aggaactttt ccatggaatg cgacagttat ggttaaataa
     1681 tgaaggaaaa tttgatggag aaattccaaa cttttttgtt tataactcat tgacaatctc
     1741 aggaataaat gaataaaaaa tactgacggt aacatgcaat atacaaaatt ggggtttaat
     1801 aggttattca aagtttgtgg ctcgcatcaa gtgccgtgta tcttgaaagt aaatcgcccg
     1861 caatccctta ctgcatatag cccatccgtt agtgcgtaaa cccccttttt caggctgtag
     1921 aaaaatgatt tatgagggaa aggattatta aatgctgata tgattgcttt aacaagcaaa
     1981 ccactgatta caaatcggca ggagcaaagg aagaatataa tcgtttgatt aaaagcatat
     2041 tacataaaaa tttaccaaac tattgaaata gattatcacc tttaaaattg aataatatgg
     2101 aaaatatgtt tatgaaattt gttaataaaa acaaatggtt tgtggttttc ttccttgttt
     2161 ggacattctt gcatgtaatt tttttgttca atggtaatag tgaattttac atagaggaat
     2221 tttggccatt tggtgataat ggcttaaggg attatggatg gtttgagttt tttgtttatg
     2281 aagctttacc actcctaatt ttcgtaattg ttaaacttgt tggcaaagac attaaaagga
     2341 aaataaaaga cattaaaagg aaaataggcg atatttatca atttacaatt ttaaatcatt
     2401 tatttctcac tgcaatgaat aaaacgatgc aaaccattat gccacatgtg gtggctgtga
     2461 ttttgtttct gataatttct ttctggtatt tcagccctgt attggaggga aaacaattga
     2521 tcggtcatga caccaaaagc tggatgtgca tggcaaaaga aacgattgac tacaacaaaa
     2581 ctcataaaga taaaacgctt tggaccaact ccatgtttag tggcatgccc acctatcaaa
     2641 tttccatgac acagccaaat aatttcagca agtatgtgga tcaatttttc agattatttc
     2701 ccgctcctgt tttttatcta attctatatt tattgggtgc ttatgttctt ttattagcat
//
LOCUS       DAOSAS010000178         2752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_23834_length_2752_cov_12.591027, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000178 DAOSAS010000000
VERSION     DAOSAS010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23834_length_2752_cov_12.591027"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..397)
                     /gene="ssrA"
                     /locus_tag="PLR72_07045"
     tmRNA           complement(1..397)
                     /gene="ssrA"
                     /locus_tag="PLR72_07045"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(467..1987)
                     /gene="rny"
                     /locus_tag="PLR72_07050"
     CDS             complement(467..1987)
                     /gene="rny"
                     /locus_tag="PLR72_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOK36990.1"
                     /translation="MELIIGIIIALAVGVTGTFLYFKQNNKRMLKKMEEEAELVKKNN
                     LIEVKEKFIALKSEYEQQVQQRNAKLQASEVKLQQREMQLNQRQGELQRKMNEIESMK
                     ENLESQFIATENRKKELEHLIRQAQEQLETISGLSTEQAKEQLIESLKEEAKTEAMSY
                     VNDIVDEAKMTANKEAQKIIIQTIQRVATETAIENSVTVFHIESDEMKGRIIGREGRN
                     IRALEAATGVEIIVDDTPEAIVLSAFDPVRREIARLSLHQLVADGRIHPARIEEVVNK
                     VKKQLEDEIIEVGKRTTIDLGIHGLHPELIRLIGKMKYRSSYGQNLLQHARETANLCA
                     IMAAELGLNPKKAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKYKEKPDICNAIGAH
                     HDEIEMETLIAPIVQVCDAISGARPGARREIVEAYIKRLKDLEEVALSYPGVVKTYAI
                     QAGRELRVIVGADKMDDKEAELLSMDIAKRIQDEMTYPGQIKITVIRETRAVSYAK"
     gene            complement(2157..2453)
                     /locus_tag="PLR72_07055"
     CDS             complement(2157..2453)
                     /locus_tag="PLR72_07055"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOK36991.1"
                     /translation="MMDNDTFLINVQAGGFKLPLRIARKDEEIYRKAEKLFSKYLEKY
                     QQKYHQRANEEILTLVAYQFAVLLIKQEMRQDVSPVIEKLTSLNKEIENCLSRK"
     gene            complement(2461..>2752)
                     /locus_tag="PLR72_07060"
     CDS             complement(2461..>2752)
                     /locus_tag="PLR72_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK36992.1"
                     /translation="NQYEELINVFASNLRKLISEYESLKVQNFMLKKEIELKDNELKS
                     LQQKVLELQEKINHFQIAKALAATPEESSLAKQQLSKMVREIDKCLALLLKE"
BASE COUNT          732 a          579 c          445 g          996 t
ORIGIN      
        1 gtggagctgg agggatttga accctcgtcc aaacaaggaa ccgatatgtt ttctacatgc
       61 ttagcttcgc ttgatttttc gtgtacaggc aaggccgaag ctgccgacct gtaccttatc
      121 ccttatgatt ttcattgcgt ttacaaggct aaacgcaact atcctcgatt tcgctgcacc
      181 tccacatcag gctgcctcga ggagagggcg cctgggagat gtctcgtccc cacaccttgt
      241 gcagggataa agcttaatct actttacttc gattaagcag cgagagcgta attagtttcg
      301 ccagttactt tttgagatcc gagatttacg agctgtgacc tcccggctcg gcatgcttac
      361 ataccctttc aacttgctgt caaaaccaaa cagccccata aaaggatata tattcaaaat
      421 aaatttacaa cgttttgtaa atcactcatt cactcttcag acaattttat tttgcataac
      481 ttacagccct cgtttcacga atgactgtaa tttttatttg accaggataa gtcatttcat
      541 cctgaatcct ttttgcaata tccattgaga gcaattctgc ttccttatca tccattttat
      601 cagcacctac gatcactctc aattctcttc ctgcctgaat ggcataagtt tttactactc
      661 caggatacga aagagcaact tcctccaggt ctttcaatcg tttaatgtat gcttccacaa
      721 tttcacgtct cgctcccggc cgtgctcctg atatagcgtc acaaacctgt acaatcggag
      781 caattagtgt ttccatttct atttcatcat ggtgtgcacc aattgcatta caaatatcag
      841 gtttttcctt atatttctca gcaagtttca taccaagaat agcatgtggc agttcgggtt
      901 catcatcggg tactttacca atatcgtgta ataaaccagc cctttttgct tttttaggat
      961 tcaatcctaa ttctgctgcc atgatcgcac aaagattagc agtttcgcgt gcatgttgca
     1021 acaaattttg accatacgaa gagcgatatt tcattttccc aataagacga attaattcag
     1081 gatgcaatcc atgaattcct aaatcaatgg tcgtacgttt acccacttca atgatttcat
     1141 cttccagttg ttttttcacc ttattgacca cctcttcaat gcgagctgga tggattcgtc
     1201 catcagccac cagttgatgt agcgacaatc gggcaatttc acgtcgaaca ggatcgaaag
     1261 ctgaaagaac aattgcttca ggtgtgtcgt ccacaataat ttcgacccca gtagctgctt
     1321 ccagtgctcg aatattacga ccttcacgac caataattcg tcccttcatt tcgtcggatt
     1381 caatatggaa aacagtaaca gaattttcga tagccgtttc agtagccaca cgctggatgg
     1441 tttgaatgat aatcttttga gcttctttat tagcagtcat ttttgcttca tctacaatgt
     1501 cattgacata agacatggct tctgttttgg cttcttcttt tagtgattca attaattgtt
     1561 ctttcgcttg ctccgttgaa agacctgaaa tagtttccag ctgttcttgt gcttgccgaa
     1621 taagatgctc aagctctttt tttcgatttt cggttgctat gaattgactt tccagatttt
     1681 ctttcatcga ttcaatttca ttcatctttc tttgcagttc accttgccgt tggttgagtt
     1741 gcatttctct ttgctgcaat tttacttcgc tggcttgcaa ttttgcattt cgttgctgaa
     1801 cttgttgttc atattccgat ttcagagcaa taaatttctc ttttacttcg attaaattgt
     1861 tttttttgac gagttcagct tcctcttcca tttttttaag catacgttta ttgttctgct
     1921 tgaaatatag gaaggtaccc gtcacaccca ctgctaatgc aataataatt cctatgatga
     1981 gttccatttt ttgattttat tatttagtaa aatttgataa atagaaatat ataaatatat
     2041 aaatataaaa aaacgcattc acgttgttta aataatcatt tttattggag tttcactcca
     2101 ataaaacaac gcaatgcggg aaatttagtt taggaaaacg ataaaaattt tactgattat
     2161 tttctactta agcaattttc aatttcttta ttcaatgatg ttaatttttc aatcacagga
     2221 gaaacgtcct gcctcatttc ttgctttatt agtaaaactg caaattgata agcaactaaa
     2281 gttaaaatct cttcattggc tcgctgatga tatttttgct gatatttttc aagatatttt
     2341 gaaaataatt tttcagcttt tcggtatatt tcttcatctt tccgggcaat acgaaggggt
     2401 aatttaaaac cacctgcttg tacatttatt aaaaaagtat cgttgtccat catcttgctc
     2461 ttattctttt aataaaagag ctaaacattt gtcaatttcc cgcaccattt tactaagctg
     2521 ctgctttgcc aagctacttt cttcgggcgt agctgctaat gctttagcaa tttgaaaatg
     2581 gttaattttt tcttgtagtt ccaaaacttt ttgttgaaga gattttaatt cgttgtcttt
     2641 taactctatt tcttttttca acataaagtt ctgtactttt aatgactcgt actccgaaat
     2701 taattttcgc agattcgaag caaatacgtt tattagttcc tcgtactgat ta
//
LOCUS       DAOSAS010000179         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_24529_length_2696_cov_13.538432, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000179 DAOSAS010000000
VERSION     DAOSAS010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24529_length_2696_cov_13.538432"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLR72_07065"
     CDS             complement(<1..575)
                     /locus_tag="PLR72_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOK36993.1"
                     /translation="MATIKGKVWQVIGPIVDVFFPLDEKNEYKLPPINNALSISLPHE
                     KDLILEVQQHIGENIVRTIAMDSTDGLRRGLEVIDLGRPISMPIGDQIKGRLLNVIGE
                     PIDGLIPLSKNSVSPIHCEPPKFEELTTTDEILYTGIKVIDLLAPYVKGGKIGLFGGA
                     GVGKTVIIMELINNIAKGHKGYSVFAGVGERT"
     gene            complement(711..2498)
                     /gene="lepA"
                     /locus_tag="PLR72_07070"
     CDS             complement(711..2498)
                     /gene="lepA"
                     /locus_tag="PLR72_07070"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOK36994.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLEYTHTVVGKDLHDQVLDNM
                     ELERERGITIKSHAIQMNYQYGGEEYILNLIDTPGHVDFSYEVSRSIAASEGALLVVD
                     ATQGIQAQTISNLYMALEHNLTIIPVINKIDMDSARPDEVEDEIVELLGCSREEIIRA
                     SAKTGEGVKEILKAIVTRIPPPQGNPEAPLQCLIFDSVFNPFRGIIAYFKIVNGTINK
                     GDWVKFVSTGKEYFADEIGVLKLNFLPMESLSAGNVGYIISGIKTSREVKVGDTITHV
                     KRPCQEAIAGFEEVKPMVFAGVYPIDTEDFEDLRSSIEKLQLNDASLTFEPETSIALG
                     SGFRCGFLGMLHMEIVQERLDREFNINVITTVPNVSYWVYTKKGEKIEVNNPASMPDP
                     ALIDHIEEPYIIASIITPTEFIGQIMTLCLGKRGELIKQNYVSGNRIELIYDMPLGEI
                     VFDFYDKLKSISRGYASFDYHMNGYRPSRLVKLDILLNGEPVDALSSLIHADNAMTLG
                     RRMCEKLKELIPRQQFEIAIQAAIGSKIIARETIKPVRKDVTAKCYGGDITRKRKLLE
                     KQKKGKKKMKQIGSVEIPQKAFLAVLKLD"
BASE COUNT          876 a          548 c          395 g          877 t
ORIGIN      
        1 gtacgttcac caacaccagc aaaaacagaa tatcctttat gtcctttggc tatgttatta
       61 ataagttcca ttataataac agtcttacca acaccagccc caccaaaaag accaattttc
      121 ccacctttta cataaggcgc aagcaagtcg ataactttaa tacctgtata taaaatttca
      181 tcagtcgttg taagttcttc aaatttagga ggctcacaat gaataggaga aacactattt
      241 ttactcaaag gaattaaacc atcgataggt tcaccaatta catttaataa tcgtcctttt
      301 atttgatctc caataggcat ggaaataggt cttcccagat caattacttc caatccacgg
      361 cgtaaaccat cagtagaatc catcgctatg gttcgaacaa tattctctcc gatatgttgc
      421 tgtacttcta atatgagatc tttttcatga ggaagtgaaa tgcttaaagc attattaata
      481 ggaggtaatt tgtattcatt tttctcatcg agagggaaaa agacatccac tatcggacct
      541 ataacttgcc acactttccc ctttattgtt gccatttttt acaaaaaatt taatttttta
      601 ttctcattct caaaaattgt ggcaaatgta gccaaaactt tattcctaaa taaaaaagaa
      661 tgtttaaata tactcaaaat tattcatttt aatgttaaaa aatttgtccc tcaatctaac
      721 tttaaaacag ccaaaaatgc tttttgtgga atttctaccg aaccaatttg cttcattttt
      781 ttcttacctt ttttctgctt ttctaataat ttccttttac gagtaatatc gcctccgtaa
      841 catttggctg taacgtcttt tctgactggt tttatagttt ctcttgcaat aatttttgaa
      901 ccaatggcgg cttgaatagc aatttcaaac tgttgtcgcg gaatcagttc ttttaatttt
      961 tcgcacatcc gtcgtcccaa tgtcatagca ttatctgcat gaatcaaaga agaaagagca
     1021 tcgaccggct caccattgag aagaatatct aactttacca accgggaagg acgataacca
     1081 ttcatgtgat aatcgaaaga agcataccct cttgaaatac ttttgagctt atcataaaaa
     1141 tcaaaaacaa tttcacctaa tggcatgtca taaatcagtt caattctatt cccagaaaca
     1201 taattctgtt tgattaattc accgcgtttt cctaaacaaa gagtcattat ctgtccgata
     1261 aactcagtag gcgtaatgat ggaagctatg atgtatggtt cttcaatatg atcaatcaaa
     1321 gcaggatcgg gcatactcgc aggattattt acttctatct tttctccttt tttggtataa
     1381 acccagtaag aaacatttgg tacagtagta atgacattta tattaaactc tctatcgaga
     1441 cgttcctgaa caatttccat gtgcaacata cccaaaaaac cacacctaaa gccagagcct
     1501 aatgctatgg aagtttcagg ttcaaatgtc agtgaagcat cgttcaattg caatttttct
     1561 attgaagagc gtaaatcttc aaagtcttct gtatcaattg gataaacacc agcaaaaacc
     1621 ataggcttta cttcttcaaa acctgctatt gcttcctgac aaggacgctt tacatgggta
     1681 atggtatcac ccacctttac ttctcttgaa gttttaattc ccgaaataat ataacccaca
     1741 ttacctgcac ttaaactttc cattggtaga aaattaagtt tcaaaacacc aatttcatca
     1801 gcaaaatatt ctttcccggt cgaaacgaat ttaacccaat cgcctttgtt gattgttcca
     1861 tttacaattt taaaataagc aataatcccg cgaaaaggat taaaaacaga atcgaaaatc
     1921 aaacattgaa gcggagcttc aggattgcct tgcggaggcg gaattcgagt aacaatagct
     1981 tttaaaattt ctttcacacc ttcgccggtt tttgcactgg cacgaataat ttcttcacgg
     2041 ctgcaaccta acaattccac tatttcatct tcaacttcat caggtctggc actgtccata
     2101 tctatcttgt taatcacagg aataatagtt aaattatgct ccagagccat atagagattt
     2161 gaaatggttt gtgcctgaat accctgtgta gcatctacca cgagcaaagc tccttcgctt
     2221 gcagcaatgg aacgcgatac ttcataggaa aaatctacat gccctggcgt atcaatcaga
     2281 tttaaaatat attcttctcc accgtattga taattcattt gaatggcatg actttttatg
     2341 gtgattcccc gttctcgttc cagctccata ttatcaagca cttgatcgtg aagatctttg
     2401 ccaacaacag tatgggtata ttccaataaa cggtcagcca acgtgctctt accgtggtca
     2461 atatgagcta tgatacaaaa atttcggata ttcttcatta gtttttattt taaatacaaa
     2521 attacaaaaa aacaaggaaa tagaaaagta gcaatttttg tgtaaagaac tgacttttgc
     2581 ttgatttata caaggcttgc tgaacaaaat atctttacgt ttaactcgat agacgttttc
     2641 aggaaagtct ctactaaaac tataaaaatt tgattatcaa ctaaatattt aaaact
//
LOCUS       DAOSAS010000180         2683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_24680_length_2683_cov_8.132040, whole genome shotgun sequence.
ACCESSION   DAOSAS010000180 DAOSAS010000000
VERSION     DAOSAS010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24680_length_2683_cov_8.132040"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1893
                     /gene="pheT"
                     /locus_tag="PLR72_07075"
     CDS             <1..1893
                     /gene="pheT"
                     /locus_tag="PLR72_07075"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOK36995.1"
                     /translation="IRDKAIHLTKPSVENFKVQNQCLTIPVIIENTEACQRYSAVTIS
                     GVKIKESPQWLQNYLKAIGIRPINNVVDITNFILYEIGQPLHAFDADKIKGGKVIVKN
                     LPEGTTFITLDGVERKLSAEDLMICNVEEPMCIGGVFGGLNSGVTETTQNIFIESAYF
                     NPVSIRKTARRHGLNTDASFRFERGCDPENTIYALKRCALLIQEIAGGEISSEIIDVY
                     PKPIRPAEVRLLYEKIDNLIGEKIERSVINTILRALEIKIMEINEEGYYLRIPLYRVD
                     VQRDVDVIEDILRIYGYNRVEITDRIVSTLSYWQKPDTFKLQNIISEQLTAQGFNEVV
                     NNSLTKSSYYVPLYSFPLENCVKILNPLSSDLSVMRQTMLFGGLENIIYNIHRQNFDL
                     KFYEFGNCYFYFPQNKRENDILSPYREEFHLALWLTGNKHSQSWIMPQEKTTVFELKA
                     YIENIFLRLGIKRRQLHVQEFSDDLLSKALVYLNEKGKKIGIFGIVHSNVLKKFDIEQ
                     EVYYADLDWNLLLALIKDHKTRFKQISKFPEVRRDLALLLDKNVNFAEIEKIAYETER
                     KLLKRVQLFDVYEGKNIAPGKKSYAVSFIMQDETKTLTDDQIDAIMKKLIKNFAEKLG
                     AQLRDK"
     gene            complement(1915..2220)
                     /locus_tag="PLR72_07080"
     CDS             complement(1915..2220)
                     /locus_tag="PLR72_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOK36996.1"
                     /translation="MIKHIVLFQLAEVAEGKTKKENAQFIKTELEKLINVIPQLKKIE
                     VGLNHPESPQNNYDIALYCEFNSLDDLNIYQEHPAHKEISEYVGKIKTSRVAVDYEV"
BASE COUNT          900 a          388 c          470 g          925 t
ORIGIN      
        1 atcagggata aagccattca tttaacaaaa ccttccgttg aaaattttaa agtacaaaat
       61 cagtgtttaa ctattccagt aataattgaa aatacagaag catgccagcg ttattcggct
      121 gtaactattt caggagtgaa aataaaagaa tcaccccagt ggttgcaaaa ttatttaaaa
      181 gccattggaa ttcgtccgat aaataatgta gtcgatataa ccaattttat tttatatgaa
      241 atagggcaac ctttacatgc ttttgatgca gacaaaataa aaggaggcaa agtaatagta
      301 aaaaatttgc ctgaaggaac gacttttata acactcgacg gggtagaacg taaacttagt
      361 gctgaagatt taatgatttg caatgtagaa gaacccatgt gtatcggtgg agtttttgga
      421 ggattaaatt ctggagtgac agaaactacg cagaatattt ttattgaaag tgcttatttt
      481 aaccccgttt ctattcgtaa aactgctcgt cggcatggat tgaatacaga tgcctctttt
      541 cgttttgaac ggggttgtga tcctgaaaat accatttatg ccctgaaacg ttgtgctttg
      601 ttaattcaag aaatagctgg tggtgaaatt tcaagtgaaa ttattgatgt ttatcccaaa
      661 cctattcgtc ccgctgaagt tagactactt tatgaaaaga tagataattt gattggagag
      721 aaaattgaaa ggagtgtcat caatactatc ttgagagcat tggaaataaa aatcatggaa
      781 ataaacgaag aaggttatta tttacggata cctctttatc gagtcgatgt gcagcgtgat
      841 gtagatgtaa tcgaagatat tctcagaatt tatggataca acagggtaga aataactgat
      901 cgtattgttt ctactttaag ttactggcaa aaaccagata ctttcaaact tcagaatatt
      961 atttcagaac aacttacagc tcaagggttt aacgaagtag taaataattc acttactaaa
     1021 agcagttatt atgttccact ttattcgttt ccgctcgaaa attgtgtaaa aattctcaat
     1081 cctttgagtt ctgacctgag tgtgatgcgt cagacgatgc tttttggcgg attagaaaat
     1141 atcatttata atattcatcg gcaaaatttc gatttgaaat tctatgaatt tggcaattgt
     1201 tatttttatt ttcctcaaaa caagcgtgaa aatgatattc tttcgcctta tcgagaagaa
     1261 tttcatttgg cactttggtt gacaggaaac aaacattctc aatcgtggat aatgccccaa
     1321 gaaaagacta cggtttttga actgaaagct tatatagaaa atattttctt gcgattggga
     1381 ataaaacgtc gtcaacttca tgttcaggaa ttttcggacg atttactaag caaagcctta
     1441 gtttatctga atgaaaaagg gaaaaaaatc ggaatttttg gcattgtaca ttctaacgtt
     1501 ttgaaaaaat tcgatataga gcaagaagtt tattatgctg atctggactg gaatttactt
     1561 ttagcattaa taaaagatca caaaacacgt tttaaacaaa tttccaaatt tcctgaagtt
     1621 cgtcgtgatt tggcattact gcttgacaaa aatgtaaact ttgctgaaat agagaaaatt
     1681 gcttatgaaa cggaacgaaa attattgaaa agagttcaat tgtttgatgt ttatgaaggg
     1741 aaaaatattg ctcctggtaa aaaatcttat gcagtaagtt ttattatgca agatgaaacg
     1801 aaaactttaa cggatgatca aattgatgct attatgaaaa agctaataaa aaattttgct
     1861 gaaaaattag gtgctcaatt gcgggataaa tgattaattt tttaattact tgatttacac
     1921 ttcataatcc actgctacac gacttgtttt tatttttccg acatattcag aaatttcttt
     1981 atgtgccgga tgttcctgat aaatatttaa atcgtctagg gaattgaatt cgcaatatag
     2041 agcaatatca taattatttt gaggcgattc gggatgattg agccccactt caattttttt
     2101 gagctgagga attacattaa tgagtttttc taattcagtt ttgataaact gggcattttc
     2161 tttcttcgtt tttccttcag caacttctgc cagttgaaaa agaacaatgt gctttatcat
     2221 aaaaaaaatt tattaagttg aaaggaatca actttcgaat agcgaataat taaaatattg
     2281 aagttttttg ctaacattga tatgcctttc tcgctgattt ccgcagattt ttattcgctg
     2341 attttcgctg atattattct gcgttcatct gcattttttt atctgcggtt atctgcgtga
     2401 aatattcttc attttctatt tttgaagcga ggttctgcag tttccctaaa ttcagtattt
     2461 caatcagata atttctaata ataggttgtt ttgcggagaa tcaaataaaa atttaatggt
     2521 ttggatagat caatttttta agctaaatac ttattttgaa ttattcctta ttaaatttaa
     2581 agtgctgatt atttgcatat tactttataa cttttcccat tgatacgcaa aaaataaatt
     2641 ccttgacgaa ctggaataaa aaatcgtcct tgaacaagat tgc
//
LOCUS       DAOSAS010000181         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_26236_length_2570_cov_10.166998, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000181 DAOSAS010000000
VERSION     DAOSAS010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26236_length_2570_cov_10.166998"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLR72_07085"
     CDS             <1..211
                     /locus_tag="PLR72_07085"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOK36997.1"
                     /translation="RWAELFAEGQWWWDVRRWEIGPAEAAFYKQTRHGPITWKGNESY
                     VQPIPQIELERNENIQQSAGYPTVR"
     gene            439..>2570
                     /locus_tag="PLR72_07090"
     CDS             439..>2570
                     /locus_tag="PLR72_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOK36998.1"
                     /translation="MKKSILLLFLILAGFRFVLHSETLFSPDKNFRLEFSLTQVGEPQ
                     YQLFYKEKTVILPSKMGFILNNSPAFLNGFSVQKVDTTSFDETWEPVWGEVRQIRNHY
                     NEMLVTLDQKSTQRTMKIRFRLFDDGLGFRYEFDKQPNLAYFQINDEVTQFALASDHK
                     TFWIPGDFDTNEYFYTTTKLSEVDASKVDGSSIGTHGYFAKNAVQTPLMMKSDKGLYI
                     NIFEAALVDYPVMDLLIDQKNLILVSALAPSAVGVKAFMQTPQNTPWRTIIVSDKATD
                     ILASKMILNLNEPCKIKDTSFIKPQKFIGVWWAMHVPNKWTWNYADIPNIKLKDTDWK
                     SLTPLPQHGAKTENVKRYIDFAAANGIPSVLVEGWNVGWEDWNGNWKEEVFDFVTPYP
                     DFDVKEIHRYAASKGVKIIMHHETSASATNYERRLDKAIEFMKENGYDAVKTGYVGRI
                     IPRGEYHDGQWMVNHYLRVAEKMAENHIMLDAHEPVRPTGLQRTYPNWMACEAARGNE
                     YNAWSVGNPPEHETILPFTRLMGGPMDYTPGIFEIKMNYYQPGNPHQVHTTLAKQLAL
                     YVTMYSPLQMAADLPENYEKRMDAFQFIKDVAVDWDDTKYIEAEPGDFITVARKAKNS
                     DDWFVGAITDENARMAVIPFNFLDTKQWYVATIYGDAANADWKENPMAYKIQKFLVNS
                     KSILKQKLAPSGGAAISLKKATKEEMKG"
BASE COUNT          892 a          412 c          503 g          763 t
ORIGIN      
        1 acgttgggca gaattatttg ctgaaggaca atggtggtgg gatgttcgga gatgggaaat
       61 cggtccggct gaagcagcat tttacaaaca aacaaggcat ggaccaatta cctggaaagg
      121 taacgaatca tatgtacagc ctataccaca aatagaatta gaaaggaatg aaaacatcca
      181 gcaatcagct ggttatccta cagtaagatg ataaattgaa ttaaaaaatt tagctgttaa
      241 aattcaatgt catattgtta tcatttatat tttatttaaa actaagactt ttagctttaa
      301 atcaaataga gtgttgttta taaagcttta tatcagcagt ataaagaaaa attgtcagat
      361 atttatgtaa gtaactttat aagccatata aaaataaaat ttaaaaaaaa tttattttca
      421 tcccctataa aaatacatat gaaaaaaagt atcttattgt tattcttgat tttagcagga
      481 ttccgctttg tattacattc agaaacccta ttttctcctg acaaaaattt tcgccttgaa
      541 ttttctttaa ctcaagttgg tgaaccacaa tatcaacttt tttataagga aaaaactgtg
      601 attcttcctt caaaaatggg atttatttta aataattctc cagctttttt gaatggattt
      661 agtgttcaaa aagtggatac tacaagtttt gatgaaacgt gggaacctgt ttggggtgaa
      721 gttcgtcaaa tcagaaatca ttacaacgaa atgctggtga ccctcgacca aaaatcaaca
      781 caacgaacca tgaaaattcg ttttcgtttg tttgacgacg gattgggatt tcgttatgag
      841 tttgataagc aacctaatct ggcttatttt caaatcaatg atgaagtaac ccaatttgct
      901 ctggctagtg accataaaac cttttggata ccaggcgatt ttgatacgaa cgagtatttt
      961 tataccacca caaaactttc agaagtagat gcgtcaaaag tagatggaag cagtattggg
     1021 acgcatggtt attttgcaaa aaatgctgtg caaacaccac ttatgatgaa aagtgataaa
     1081 ggattgtata tcaatatttt tgaagctgca ttagtagatt atccggttat ggacttattg
     1141 atagatcaaa agaatttgat tctggtttca gctctggcac ccagtgctgt gggagtaaaa
     1201 gcttttatgc agactcccca gaatacgccc tggcgtacca ttattgtcag cgataaagca
     1261 actgatatcc ttgcttcaaa aatgatttta aacttgaatg aaccatgtaa aattaaagat
     1321 acaagtttta ttaaaccaca aaaatttatt ggggtttggt gggctatgca tgttcccaat
     1381 aaatggacat ggaattatgc ggatattcca aatattaaat tgaaagatac tgattggaaa
     1441 tcacttacgc ctcttccaca acatggagct aaaacagaaa atgtaaaaag atatattgat
     1501 tttgcagctg ctaatggcat accgagcgtg ctggttgaag gctggaatgt tggttgggaa
     1561 gactggaatg gaaactggaa agaagaagtt ttcgattttg tgactcctta tcccgatttt
     1621 gacgtcaaag aaattcatcg atatgctgct tccaaaggag ttaaaattat tatgcatcac
     1681 gaaacgtcag catcagctac taattatgaa cgccgattgg acaaagccat tgaatttatg
     1741 aaagaaaacg gttatgatgc agtaaaaaca ggttatgtag gaagaattat tcctcgcgga
     1801 gaatatcatg atggtcaatg gatggttaat cattatctca gagtagcaga aaaaatggct
     1861 gaaaatcata tcatgcttga tgctcacgaa cctgtcagac caacgggact tcaacgtaca
     1921 tatcctaact ggatggcttg tgaagctgct cgcggcaatg aatacaatgc ctggagtgtt
     1981 gggaatccac ctgaacatga aaccatttta ccttttacaa gattaatggg tggtccaatg
     2041 gattatacac ctggaatttt tgaaattaaa atgaactatt atcaaccagg aaatccacat
     2101 caagtgcata caacgctggc aaaacaactg gctctttacg taacgatgta tagtccacta
     2161 caaatggcag ccgatttgcc tgaaaattac gaaaaaagaa tggatgcttt tcaatttatc
     2221 aaagatgttg ccgtagattg ggacgataca aaatatattg aagctgaacc gggcgatttt
     2281 attacggtgg cacgaaaagc taaaaacagc gatgactggt ttgttggagc cataacggat
     2341 gaaaatgcac gaatggccgt tattcctttt aattttttgg atacaaaaca atggtatgtt
     2401 gctactattt atggtgatgc agcaaatgcc gactggaaag aaaatccgat ggcttataaa
     2461 attcaaaagt ttttggtcaa ttcaaaaagc atattaaaac aaaaacttgc tcccagtggt
     2521 ggtgcagcca ttagtctgaa gaaagctaca aaagaagaaa tgaaaggttt
//
LOCUS       DAOSAS010000182         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_27269_length_2498_cov_14.973393, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000182 DAOSAS010000000
VERSION     DAOSAS010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27269_length_2498_cov_14.973393"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            547..2046
                     /locus_tag="PLR72_07095"
     CDS             547..2046
                     /locus_tag="PLR72_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HOK36999.1"
                     /translation="MQNKFKFVSAEEAAAYVQHKDVVGFSGFTPAGCPKAVPLAIAKR
                     AETFYAEGKEFKIGMYTGASTGVSVDGALARSDAVLFRTPYQSNKDMRNAINAGKAPY
                     FDMHLSTLAQELRFGVLPKPTFAILEVCDWNEEGELVLTAAVGISPTIADLADHIILE
                     WNHYHPKELRGFHDIYQPQDPPYRREIPIYKPSDRIGSEILKVDPSKILCIVETNLPD
                     EVLDFAPLDEVTKQIGLNVATFLVDEIRSGRMPHGFLPIQSGVGNIANALLFSLGKNE
                     EVPPFEMYTEVIQDAVIELMIEGNIKFASGCSLTVTNDVLQKIYQNLDFFRDKLVLRP
                     QEISNNPEIVRRLGLITINTALEADIFGNVNSTHVLGTKMMNGIGGSGDFTRNSFISI
                     FTTPSTAKNGNISSIVPMVPHVDHTEHSVNVIVTEYGIADLRGKTPKERAEIIIENCA
                     APEYKQMLYDYLAISGTNHIPHNLKACFGMHIQFEKTGSMLGTNWNDYK"
BASE COUNT          808 a          397 c          444 g          849 t
ORIGIN      
        1 gactaataac ttcttttttc cctgaaggac gcggaatctg tgtcagactg taaaagtttt
       61 gccacaattg ctgcggttga agctgacgaa tatcattgtt gttcataata tgtttattat
      121 tagaattttg taaattctga atatttattc caatttttca aactacaaat atgctcaaat
      181 ttttcagata aatgaactgt tgaggagttt ttttacaaat attgagaatg aattttcaaa
      241 acgggaagta agaaaagtac aattgaaatc aattttgagt cgcttcctag aacggctcta
      301 caagaaatta ccgataaaat tccagtaaca tttctttatt taaccttttc taataattcc
      361 tgtattttag cagcgttata gttataattt ttgatattta tcttctaaat aatttttcac
      421 caataattta ttagcaaaat aaggattact cctgaaaaat ttccttaatc agttttgagt
      481 atcttgaagg attgggaaat atttgataat tttgtgcaga atttttaaga ataaactaac
      541 ttttatatgc aaaataaatt taagtttgtt agtgcagaag aagcagcagc ttacgttcaa
      601 cataaagacg tggtaggttt tagcggtttt actccggcag gttgtccgaa agcggttcct
      661 ttagcaatag ccaaacgtgc agaaactttt tatgctgaag gaaaagaatt taaaatcgga
      721 atgtacactg gagcctcaac tggagtttca gtagatggtg ctttagctcg atctgatgcg
      781 gtattatttc gaactcctta ccagtcgaac aaagacatgc gtaatgctat caatgcagga
      841 aaagctccct attttgacat gcatttatct acactggctc aggaactgcg ttttggagtt
      901 ttaccgaaac caacattcgc tattctggaa gtatgtgact ggaatgaaga aggtgaattg
      961 gtattaacag cagcggtagg aatttcgcca actattgccg atttggccga tcatattatt
     1021 ttggaatgga accattatca tcccaaagaa ttgagaggtt ttcacgacat ttatcagcca
     1081 caggatccac cttaccgcag agaaattccc atttataaac cctctgacag aattggctcg
     1141 gaaattttga aagtcgatcc ttctaaaatt ctttgtattg tagagactaa tttaccagat
     1201 gaggtattgg attttgctcc attggatgaa gtaactaaac aaattggttt gaatgtggct
     1261 acttttttgg ttgatgaaat tcgttcagga agaatgccac atggtttttt acctattcag
     1321 tcaggtgttg gtaacattgc caatgctttg ctgttttctt taggcaaaaa tgaagaagtt
     1381 ccaccttttg aaatgtatac tgaagtgatt caagatgccg tgattgaact gatgatagaa
     1441 ggaaacatta aatttgccag tggttgctcg ctgacggtta ccaatgatgt attacagaaa
     1501 atatatcaaa atcttgattt tttccgcgat aaattagtgc ttcgtcctca agaaatttct
     1561 aataatcctg aaatagttcg ccgtttgggt ttgattacta tcaatacagc actggaagct
     1621 gatatttttg ggaatgtaaa tagcactcat gtacttggaa ctaaaatgat gaatggcatt
     1681 ggaggttcag gcgattttac acgcaattca tttatttcta tttttaccac tccttccact
     1741 gccaaaaacg gcaatatcag cagtattgtt ccgatggttc cacatgtgga tcatactgaa
     1801 cattcagtaa atgttattgt taccgaatat ggaatcgctg acttacgggg taaaacacca
     1861 aaagaaagag cagaaattat tatagaaaat tgtgcggcac cagaatataa acaaatgttg
     1921 tatgattatc ttgcaatttc tggtaccaat cacataccac ataatttgaa agcttgtttt
     1981 ggcatgcata tccaatttga aaaaacaggt agtatgctgg gaaccaactg gaatgattat
     2041 aaatgacaaa tattttttga ctttttgaaa aggatcaaaa ctaaaatatg tgatatccta
     2101 atacactttg agcatgaaaa gcattctgaa aagtcagtct gaatttttat tcatgtactc
     2161 aatgaaaatt atcatgtaaa tttgtgtagc attttttctt cgtaaaagca ttcttattta
     2221 aaataatgaa ttttttccta tttccataag tttctaaaaa attataaagt taacatattg
     2281 atttaaagaa cattattgaa tttgaatgtc cggtatggta cacaccaacg agatttccct
     2341 tgcaaaatat cgatctgcaa gttcttctaa atggttaagg aagtagcctg ctgtttttcc
     2401 aacaaattta tttgttccat acatcctttg tgaacgtcaa tggcacatgc tttttccata
     2461 aaattaccgg attaaattgt taaacaatta gtttattt
//
LOCUS       DAOSAS010000183         2464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_27776_length_2464_cov_11.549191, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000183 DAOSAS010000000
VERSION     DAOSAS010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27776_length_2464_cov_11.549191"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PLR72_07100"
     CDS             complement(<1..810)
                     /locus_tag="PLR72_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HOK37000.1"
                     /translation="MKQDKILWIDDEIDLLQPYLIFLNDKGYEVLTATNGNDAIEICQ
                     QENFDIIFLDENMPGLTGLDTLAVIKELLPDIPVVMITKNEEENIMNMAIGNKIADYL
                     TKPVNPSQILLTLKKNIHIKEIVTEHATSTYRSEFSQIGMKINDSLTFEEWIDIYKKL
                     VYWELELSETDSGMDEMLQMQKTDANTAFAKFIKTNYLKWFENPETRPLISPDLFKTK
                     IFPLLDKGEKIFFVVVDNFRYDQWKIIRDLLGDFYQFEEEELYCSILPTATQ"
     gene            complement(848..>2464)
                     /locus_tag="PLR72_07105"
     CDS             complement(848..>2464)
                     /locus_tag="PLR72_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK37001.1"
                     /translation="EMATAMFNLGMIYYDKLQDVPMAIKTFEEFVSRFKQHPRLSEAY
                     FQLYLMESKMGNRDKAEQWRTKLITEFPETTFARLLSKPDYPERLKRMYQEQDSIYRL
                     AYQAYTKNDFNAVFQYKEYMEQNYPTSPLMPKFLFLEALSIGKTSPSERFEKELNNLI
                     SAYPESDVSSMAKDILALIKQGKQVQTSSTHGSLLAKREEIIKTEKETVVQQQFSPDK
                     KTKHLLLLISDADKRDLNQLMYQLASFNFSRFLIKDFDIGIQPLDSTRNILFVTNFDS
                     YDEVLWYLNTLSSDELLQSLMNQLNIQKVIISENNFALLKTTFSLNDYLAFHDKFLTP
                     DNKAIKEITASFEKKTASTVISSSAAGKPQIGGETQSPAKALKNENVLTQSVAPTLPL
                     EKQTEAALSSTTPPITKEEVPLYKGLFAYKPNELHFVTIYVVSGNVDETKVQNAFDKY
                     NQQNYGILNLSTRAEKVGKQQFFIIGPFPDANTAKSYLMRIVKESSLFEGLKGANYRN
                     LVGTQENLNTMLQQNALNVYFDFMKEYYLK"
BASE COUNT          785 a          418 c          382 g          879 t
ORIGIN      
        1 ttgagtagca gttggcaaaa tactacaata aagctcttct tcttcgaatt gataaaaatc
       61 gcccaaaaga tctcgaataa ttttccattg atcatagcga aagttatcaa ccaccacaaa
      121 aaaaattttt tcacctttat caagtaaagg aaaaattttt gttttgaata aatcaggact
      181 tatcaagggg cgagtttcag gattttcgaa ccatttaaga taattggttt tgataaattt
      241 tgcaaaagct gtattggcat ctgttttttg catttgtaac atttcgtcca tcccactatc
      301 cgtttcggat agttccagtt cccagtaaac caatttctta taaatatcaa tccattcttc
      361 aaaagttaaa gaatcgttga ttttcattcc aatttgagag aattctgaac gataagtgga
      421 agtagcatgt tcggttacta tttcttttat atgaatattt tttttcaaag ttaataaaat
      481 ctgactggga ttcactggtt ttgtaagata atcggcaatt ttgttcccga tagccatgtt
      541 cataatattt tcctcttcat tttttgtaat catcactacc ggaatatcag gtaataattc
      601 tttaattaca gccagtgtat cgagcccagt caaaccaggc atattttcgt ccagaaaaat
      661 aatatcgaaa ttttcttgct gacaaatttc aatggcatca ttgccattgg tagcagttaa
      721 aacctcatac cctttgtcat taagaaaaat aagataaggc tgcaacaaat caatttcatc
      781 gtctatccag agtattttgt cttgtttcat aacccaataa aattttgttc atcgaaaagc
      841 ataaaaatca ttttaaataa tattctttca taaaatcaaa atacacattc aatgcatttt
      901 gctgaagcat agtattcaaa ttttcctgcg ttcctactaa attacgatag tttgcacctt
      961 ttaatccttc aaaaagcgaa gattctttga ctattcgcat caaatacgat tttgcagtgt
     1021 tggcatcagg aaaaggtcca atgatgaaaa attgctgttt cccaactttc tcagctcgag
     1081 ttgacaaatt caaaatccca tagttttgtt gattatattt atcaaaagca ttttgaactt
     1141 ttgtctcatc cacatttcct gaaacaacat aaatagtaac aaaatgaagc tcatttggtt
     1201 tataagcaaa taaacctttg taaagaggca cttcttcttt agttattgga ggtgttgtcg
     1261 aagaaagagc tgcttctgtt tgtttttcca gtggtagagt tggtgcaacc gattgagtta
     1321 aaacattttc atttttcaat gcttttgctg gcgattgagt ttcacctcct atttgtggtt
     1381 tcccagcagc agaagaagag attactgttg aagctgtttt tttctcaaaa gacgcagtaa
     1441 tttcttttat tgctttattg tcgggtgtta aaaatttatc gtggaaagcc aaataatcgt
     1501 ttaaactgaa agttgttttc aataaagcaa aattattttc agagataata accttttgta
     1561 tattcaattg attcataagt gattgtagta attcatcaga cgaaagtgta ttcaaatacc
     1621 acaacacttc atcataagaa tcgaaattgg taacaaataa aatatttctt gtgctatcca
     1681 gtggctgaat gccaatgtca aaatctttaa ttaaaaatct agaaaaattg aacgaagcaa
     1741 gttgatacat caattgattc aaatctcttt tatctgcatc gcttatcagc aacagcaaat
     1801 gtttggtttt tttatcaggc gaaaactgtt gttgaaccac tgtttctttt tctgtcttaa
     1861 ttatttcttc ccgtttagct aaaagagaac catgtgtact acttgtttgc acttgttttc
     1921 cttgttttat caaggctaaa atatcttttg ccatcgagct cacatcgctt tcaggataag
     1981 cactgattaa attgtttaat tctttctcaa aacgttcaga tggagatgtt ttaccaatac
     2041 ttaaagcttc aaggaacaaa aattttggca taaggggtga agtaggataa ttttgttcca
     2101 tatattcttt atactgaaat acggcattaa aatcattctt tgtataagct tgataagcaa
     2161 gacgataaat agaatcctgc tcttgataca ttcgttttaa tcgttcagga taatcaggtt
     2221 tggaaagcaa tctggcaaaa gttgtttcag gaaattcagt aattaatttt gttcgccatt
     2281 gttcagcttt atctcggtta cccattttgc tttccatcag atacaattgg aaataagctt
     2341 ctgataagcg tggatgttgc ttaaatcggc taacaaattc ttcaaaagtt ttaatcgcca
     2401 ttggaacgtc ctgcaacttg tcataataaa tcattcctaa attaaacatg gctgttgcca
     2461 tttc
//
LOCUS       DAOSAS010000184         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_27828_length_2461_cov_6.997506, whole genome shotgun sequence.
ACCESSION   DAOSAS010000184 DAOSAS010000000
VERSION     DAOSAS010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27828_length_2461_cov_6.997506"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..473)
                     /locus_tag="PLR72_07110"
     CDS             complement(66..473)
                     /locus_tag="PLR72_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOK37002.1"
                     /translation="MAKEVVQNSGIIYNSLTNGSKIIGEIIADNDFRIDGEVEGKITC
                     KGKVIIGQKGFMKGSITCSNTEILGKVEGELIVFDTLTLRETAIVNGDVKTKVFVIEP
                     NAIFNGSCSMQNNLEKQTKENPKEVKKSEYQSN"
     gene            921..2174
                     /locus_tag="PLR72_07115"
     CDS             921..2174
                     /locus_tag="PLR72_07115"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOK37003.1"
                     /translation="MDNNNINWKEAALKASQALHFVNDKTIRDVLLDLTEETLKQKKE
                     ILSANQKDLSAMDRTNPMYDRLLLDEQRLENIATDIRNVAALPSPLGKILMETIRPNG
                     MKISKVTVPFGVIGIIYEARPNVTFDVFSLCLKSGNVCVLKGGTDAYHSNEAIVKIIR
                     SVLQKFDLDNHIVTLLPAGREATSELLNAVGYVDLIIPRGGKGLIEFTREHSKIPVIE
                     TGAGVCHTYFDEFGEVEKGSKIIFNAKTRRVSVCNALDCLIIHERRLNDLPILCQKLA
                     EKNVIIYADAKAYTFLVGKYPISLLQKATEESFGTEFLDYKMAVKTVESIQEAIQHIS
                     TYGSKHSECVISENKENIALFDTMVDAACVYTNVSTAFTDGAQFGLGAEIGISTQKMH
                     ARGPMALNELCTYKWIIKGNGQTRE"
BASE COUNT          881 a          374 c          365 g          841 t
ORIGIN      
        1 ttttaatttt aatgtagctg gagaaaacta aaaatcaact aatgttaaat gagtagtatt
       61 aaacattagt ttgattgata ttctgatttt tttacttctt ttggattttc ttttgtttgt
      121 ttttcaagat tattctgcat tgagcaggag ccgttaaata ttgcatttgg ctctattaca
      181 aacactttag tttttacatc cccattaaca atggctgttt ctcggagagt caaagtatca
      241 aaaacaataa gttctccttc cactttacct aaaatttcgg tattgctaca agtaatagaa
      301 cctttcataa aacctttttg cccaataatt actttccctt tgcaagtaat ttttccttct
      361 acttcaccat cgattctaaa atcattatca gcaatgattt ctccaataat tttactcccg
      421 tttgttaacg aattgtaaat aatacctgaa ttctgaacaa cttcttttgc catgattata
      481 ataaattttt tctttcaaaa ttacaccatt tttttagaaa aaaattaata ctttcccaaa
      541 ttatttcttt ttctgaatga aataatagtt ttttaaaaac ctaaaatcac aactaaaaaa
      601 ttgagcctac tcttaaaaac ctattttgta attatttctt aaatttaata ttgatattca
      661 caacttacaa attacaaaac tatcaaacta atgttcataa aaaagactga tataggcaaa
      721 gataatatat tttctactat ccaacaataa aaaagtttat tgaattgttg atgattcttt
      781 attatttttt actccaaaaa ttaccaagaa gcaattaatt tatttaaaat attgatttaa
      841 ttttgttgtt attcttgtta agtgatactt ttgtaagata aaatctgctt aagaaatttt
      901 atttttaatt ttttaaaatt atggataata acaacataaa ttggaaagaa gctgctctaa
      961 aagcttctca ggcacttcat tttgtaaatg ataaaaccat tcgtgatgtt ttattggatc
     1021 tgacagagga aaccttaaaa caaaaaaagg aaatcttatc agccaaccaa aaagacctgt
     1081 cggcaatgga tcggactaat ccaatgtatg acaggctttt attagatgaa cagcgtcttg
     1141 aaaatattgc tacagatatt cgtaatgtag cagcattacc ttctccactt ggaaaaattt
     1201 taatggaaac aattcgtcct aatgggatga aaatttcaaa agtaacggtg ccatttggtg
     1261 tgattggaat catttacgaa gcccgtccca atgttacatt tgatgttttt tctctttgtc
     1321 ttaaatcagg aaacgtctgt gtattaaaag gtggcacaga tgcatatcat tccaacgaag
     1381 caatagtaaa aattattcgt tccgtactgc aaaaatttga tctggataat catattgtta
     1441 ccttgcttcc tgccgggcgt gaagccacca gtgaactgct gaatgcagta ggatatgtag
     1501 atttaattat tccgcggggt ggaaaagggt tgatcgaatt tactcgcgaa cattccaaaa
     1561 ttccagtaat agaaacaggt gcaggtgttt gtcatactta ttttgatgaa tttggcgaag
     1621 tagaaaaagg aagtaaaatt atttttaatg caaaaacccg tcgagttagc gtttgtaatg
     1681 ccctcgactg ccttattatt catgaaaggc gactgaacga tctccctatt ctatgtcaaa
     1741 aattagcaga aaaaaatgtg attatttatg cagatgcaaa agcttacact tttttggtag
     1801 gcaaatatcc tatctctttg ttgcaaaaag ctactgagga aagttttggt actgaatttc
     1861 tggattacaa aatggctgtt aaaacggtag aatccattca ggaagctatt cagcatattt
     1921 caacatatgg ttcaaagcat agtgaatgtg taataagcga aaataaagaa aatatagctc
     1981 tttttgacac aatggttgat gcagcttgtg tttataccaa tgtttctaca gcttttactg
     2041 atggagctca atttggattg ggagcagaaa ttggcatcag cactcagaaa atgcatgccc
     2101 gtggtccaat ggctttaaat gaactatgca cttacaaatg gatcattaaa ggaaacggtc
     2161 aaacacgaga ataaaaaaat tgtttttaat ttatagaggt aaactataat taattaaaag
     2221 aaaaaacttt tttatcaacg gctataaaaa aaattaacaa agtactgata atcaaaaaat
     2281 taatgtgata tgctttataa acaaaaaata attctactgg ttttgatcac aataaatcaa
     2341 aagaaatttt aatcgaatat tattaaaaaa attcttaacg ataatttggg attattcaaa
     2401 ttaattattt caaaaccatg acaggaataa aagtacataa aaaaccaact acgaagcctg
     2461 c
//
LOCUS       DAOSAS010000185         2443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_28137_length_2443_cov_11.723199, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000185 DAOSAS010000000
VERSION     DAOSAS010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28137_length_2443_cov_11.723199"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /gene="rplB"
                     /locus_tag="PLR72_07120"
     CDS             complement(<1..494)
                     /gene="rplB"
                     /locus_tag="PLR72_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOK37004.1"
                     /translation="MAVRKLVPTTPGQRNKIISTFDTITAKEPEKSLVIGKKKTGGRN
                     NGGRRTMRYIGGGHKRKFRIIDFKRNKDGVPATVKAIEYDPNRSAHIALLYYADGEKS
                     YILAPEGLKVGQKVQSGPEVEPEVGNALPLANIPLGTIIHNIELRPGQGASMARSAGT
                     FAQL"
     gene            complement(504..797)
                     /gene="rplW"
                     /locus_tag="PLR72_07125"
     CDS             complement(504..797)
                     /gene="rplW"
                     /locus_tag="PLR72_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOK37005.1"
                     /translation="MENIIIKPLVTEKLTKQTEKFNRYGFKVNRDANKIQIKEAVEAL
                     YNVTVIKVNTMIVAPKKRSRYTKSGIIEGEKSAYKKAIVTLKKGEEIDFFSNI"
     gene            complement(805..1437)
                     /gene="rplD"
                     /locus_tag="PLR72_07130"
     CDS             complement(805..1437)
                     /gene="rplD"
                     /locus_tag="PLR72_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOK37006.1"
                     /translation="MELSVLNIKGEDTGRKVSLNEKIFSIEPNDHAIYLDVKQHLANR
                     RQGTHKTKVRSEVTGSTRKLGRQKGGGGARPGDIKSPLRIGGGTIFGPVPRSYSFKLN
                     KKLKQLARKSALSYKAKDQQITVVEDFDFDTPKTKKLIEIIKNLQVADKKPLIVLENA
                     KKNVYLSARNIPALKVVTASELNTYILLNAKMLVLTENSISAIEKTFNLE"
     gene            complement(1440..2057)
                     /gene="rplC"
                     /locus_tag="PLR72_07135"
     CDS             complement(1440..2057)
                     /gene="rplC"
                     /locus_tag="PLR72_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOK37007.1"
                     /translation="MPGLIGKKIGMTSVFSEDGKNVPCTVIEAGPCFVTQVKTKEKDG
                     YEAVQLGFQTKNEKHTIKSEMGHFKAAGVLPQRHLVEFKDFEKELKPGDVLTVEIFEE
                     NSYVDIIGTSKGKGYQGVVKRHNFSGVGETTHGQHNRQRAPGSLGASSFPSRVFKGMK
                     MGGRMGGNRVTVQNLLVLKVIPEHNLLVVKGSIPGAKNSIVIINK"
     gene            complement(2147..>2443)
                     /gene="rpsJ"
                     /locus_tag="PLR72_07140"
     CDS             complement(2147..>2443)
                     /gene="rpsJ"
                     /locus_tag="PLR72_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOK37008.1"
                     /translation="KIRIKLKSYDYNLVDKSAEKIVKTVKATGAIVSGPIPLPTHKRI
                     FTVNRSTFVNKKSREQFELSSYKRLIDIYSSTSKTVDALMKLELPSGVEVEIKV"
BASE COUNT          677 a          472 c          346 g          948 t
ORIGIN      
        1 attaattgag caaaagtccc tgctgaacgt gccattgaag caccttgacc gggacgtaat
       61 tcaatattat gaataatagt accaagtgga atatttgcta atggaagtgc attcccaact
      121 tctggttcaa cttctggtcc cgattgaact ttttgaccta cttttaaacc ttctggagca
      181 agaatgtatg atttttctcc atcagcataa taaagcaaag ctatatgagc tgatcgattg
      241 ggatcatatt caatagcttt aacggttgct ggcacgccat ctttattacg tttaaaatca
      301 ataattctga attttctttt atgaccacca ccgatatagc gcattgtcct tcgcccacca
      361 ttattgcgac cacctgtttt cttttttcca ataacaagag atttttcagg ctcttttgca
      421 gtaatagtat caaaggtact tataatttta tttctctgcc cgggtgtagt agggactaat
      481 ttacgaactg ccatgtttta tttttatatg ttgctaaaaa aatcaatttc ttcacccttt
      541 tttaaggtta caatagcttt tttataagcc gacttctctc cttcgataat tccactttta
      601 gtgtaacgac ttcttttttt aggagctact atcattgtat ttactttaat tactgttaca
      661 ttatacaaag cttctactgc ttcttttatc tggattttat tcgcatccct gtttacttta
      721 aatccatacc gattaaattt ttcagtttgt ttcgttaact tttctgtaac cagaggtttt
      781 ataataatat tttccatttt ccttttattc taaattaaaa gttttctcaa tcgcagaaat
      841 tgaattttct gttaatacta acattttagc attcagtaaa atataagtat ttaattcaga
      901 agccgttact acttttagtg cgggaatatt tcgagccgac aaatatacgt ttttctttgc
      961 attttctaaa acaattaaag gttttttatc agctacttgt aaatttttaa tgatttctat
     1021 taattttttt gtttttgggg tatcgaaatc aaaatcttct acaactgtaa tttgctgatc
     1081 tttagcctta taagaaagag ctgattttcg tgctaattgt tttaactttt tatttaactt
     1141 aaaactgtaa ctgcgaggta caggtccaaa aattgtaccc cctccaatac gaagtggtga
     1201 tttaatatca cccggtcgtg ctcctcctcc gcctttttgt ctgcctaatt tacgtgtact
     1261 tcccgtaact tcacttctaa cttttgtttt atgggttcct tggcgacgat tagccaaatg
     1321 ttgtttcaca tccaaataaa tagcatggtc atttggctca atactgaaaa tcttttcatt
     1381 caatgacact tttcgacctg tgtcttctcc tttaatattt aatacactta attccatggt
     1441 tatttattga tgattacgat tgaattttta gctccaggta ttgaaccttt tactactaat
     1501 aaattatgtt caggtatcac ttttaataca agtaaatttt gaacagttac tctattgcct
     1561 cccattcgtc ctcccatttt cattccctta aatacacgag aagggaaaga ggaagctcct
     1621 aatgaaccgg gggctctttg tcgattgtgc tgtccatgcg tggtttcacc aactccagaa
     1681 aaattatgtc gttttactac gccttgatat cctttacctt ttgaagtacc aattatatcg
     1741 acataactat tttcttcaaa aatttctacc gtaagcacat cgcctggttt taattctttt
     1801 tcaaaatctt taaattctac caaatgtctt tgtggtaaaa ctcccgcagc tttgaaatgt
     1861 cccatttctg atttaatggt gtgcttttca ttttttgttt gaaaacctaa ctgaacagct
     1921 tcatagccat ccttttcttt tgtttttact tgtgtaacaa aacaaggacc ggcttcaatt
     1981 actgtacaag gaacattttt tccatcttca ctgaaaacag aagtcatccc aattttttta
     2041 cctattaatc ctggcatttt aaatttaatt taagaccctt aaccacttgc gtgggatcgg
     2101 tcattgatta cacatttata tttatgtatt gaattaaaaa actttatcaa acttttattt
     2161 ccacttctac tccacttggc aattcaagtt tcattaatgc gtcaactgtt ttactcgttg
     2221 agctataaat atcaatcaat cttttgtaag aagaaagctc aaactgctct cttgattttt
     2281 tattgacaaa agtagagcga ttcactgtaa atatacgctt atgcgtgggc agtggtattg
     2341 gaccgcttac tatagctccg gtcgctttca ctgtttttac aatcttttca gccgatttat
     2401 caaccagatt gtaatcgtaa gattttaatt taattctaat ttt
//
LOCUS       DAOSAS010000186         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_28962_length_2394_cov_14.411287, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000186 DAOSAS010000000
VERSION     DAOSAS010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28962_length_2394_cov_14.411287"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLR72_07145"
     CDS             <1..198
                     /locus_tag="PLR72_07145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOK37009.1"
                     /translation="ESEKKLINSIKKLVLKDLIVEKEIDPEIIGGFILEIGDVRWDGS
                     LATQLNKIHKKLREGTFLNLN"
     gene            257..1846
                     /gene="atpA"
                     /locus_tag="PLR72_07150"
     CDS             257..1846
                     /gene="atpA"
                     /locus_tag="PLR72_07150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOK37010.1"
                     /translation="MPENAIKVSEISELLKKQLEQIDINVQYDEIGKVLEVSDGVARI
                     YGLTNVGANELLEFENGVMAIAMNLEEDNVGAVLLGPTYLVKEGFTVKRTDRVASINV
                     SEEMLGRVINPLGVPIDGKGSIGGDFFEMPLERKAPGVIFRQPVSEPLQTGLKAIDAM
                     TPIGRGQRELIIGDRQTGKTSITVDTIINQLDNFKAGNPVYCIYVAIGQKGSTVASLV
                     NTLKEYGAMSYTIVVTANASDPAALRYLAPYAGAAIGEYFRDTGRHALVIYDDLSKQA
                     VAYREVSLILRRPPGREAYPGDIFYLHSRLLERAAKIIAQQEVAEQMNDLPECLKGKV
                     KAGGSLTALPIIETQAGDISAYIPTNVISITDGQIFLDTDLFNEGVRPAINVGISVSR
                     VGGNAQIKPMKQVAGTLKTDQAQFRELEAFTKFGSEMDAVTAMTIDKGQKNTKLLIQP
                     LHKPLSVEKQVAILYCGTHGLLADVPLDKVKDFEDKFLTILEYSYKEEVLNEIKKGNF
                     DDKISSLIEEAAQRAQAQIGA"
     gene            1937..>2394
                     /locus_tag="PLR72_07155"
     CDS             1937..>2394
                     /locus_tag="PLR72_07155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOK37011.1"
                     /translation="MPTLKELKNRIQSVRSTQKITSAMKMVSSVKLMKAEKAINSFLP
                     YQQALSEILRNYLSNQIQTFSPYTDIRDVQHVAIVVFASNSSLCGNYNSNMIKRMEQS
                     IVTHRDVRKEDILIFPIGKKVSQACKKRNLKIAGDFEEIAAQPTFENTAP"
BASE COUNT          806 a          373 c          489 g          726 t
ORIGIN      
        1 gaaagtgaaa agaaattaat aaattcaatt aaaaaattag ttcttaaaga tttgattgtt
       61 gaaaaagaaa tagaccccga aatcattggg ggatttattt tggagatagg cgatgtgaga
      121 tgggatggaa gtttagccac ccagttgaat aaaattcata aaaaattgag ggaaggaaca
      181 tttttaaatt tgaattaatt tttttccttc ctttgaataa aaaatttatt tcattataaa
      241 atttggagat aaaaatatgc ctgagaatgc cataaaagtg agtgagatat cagagctact
      301 gaaaaaacag cttgaacaaa ttgatataaa tgttcaatac gatgaaattg ggaaagtgct
      361 tgaagtgagt gatggagtag caagaatcta tggattgacg aatgtaggag cgaatgaatt
      421 gcttgaattt gaaaatgggg taatggctat tgcaatgaat cttgaagaag ataatgtggg
      481 agcagttttg ctgggaccaa catatcttgt aaaagaaggt tttactgtta aaagaacaga
      541 tagggttgct tccatcaatg tgagcgaaga aatgcttggc cgggtaatta atcctttagg
      601 tgtgccgatt gacgggaagg gaagtattgg aggagatttt tttgaaatgc cattagagag
      661 aaaagccccg ggcgttattt tccgtcaacc agtatcagaa ccattacaga caggtttgaa
      721 ggctattgat gccatgaccc ctattggacg tggacaacgt gaattaatta ttggcgaccg
      781 tcaaacagga aaaaccagta ttaccgttga tacgattatt aatcaattag ataatttcaa
      841 agccggcaat cctgtttact gcatttatgt agccattggt caaaaaggct ctaccgttgc
      901 ttcgctggta aatacactga aagaatatgg agctatgagt tatacgatcg tagtaacagc
      961 aaatgcttct gatccggctg ccttacgtta tcttgctcct tacgcaggtg ctgctattgg
     1021 cgaatatttt cgtgatacag gacgtcatgc tttggttatt tatgatgacc tatcaaaaca
     1081 agcagtagct taccgcgaag tttccctcat tcttcgtcgt ccacccggaa gagaagctta
     1141 tcccggcgat attttctatt tacattctcg tttgttggaa agagctgcta aaattattgc
     1201 ccagcaggaa gtagctgaac aaatgaacga tttgcccgaa tgcctcaaag gaaaagtgaa
     1261 agcaggcggc tcattaacag ctttgcccat catcgaaaca caggctggcg atatttcggc
     1321 gtatattccc acaaacgtga tttctattac agatggacaa atatttttgg atactgattt
     1381 attcaatgaa ggggttcgtc cggctattaa tgtaggaatt tctgtttcca gagttggtgg
     1441 taatgctcaa ataaaaccta tgaaacaagt agccggcaca ttgaaaaccg atcaggctca
     1501 atttcgtgaa ttggaagcat ttactaaatt cggtagcgaa atggatgccg taacggctat
     1561 gactattgat aagggtcaaa aaaacacaaa acttttgatt caacctttgc ataagcctct
     1621 ttcagtagaa aaacaggttg ctatccttta ttgcggtaca catggattat tagccgatgt
     1681 tccattagat aaagtgaaag attttgaaga taaatttttg actattctgg aatactctta
     1741 caaagaagag gttttgaacg aaattaaaaa aggaaatttt gatgacaaga tttcttctct
     1801 cattgaggaa gcagctcaaa gagcacaagc acaaattgga gcataaatat tctatcttaa
     1861 aatagggctt ttgtttttca cttaatgaaa cgttgatttg gtaaattact aattttcaaa
     1921 ttaaaatttt ttatacgtgc ccactttaaa ggaattaaaa aacaggatac agtctgtaag
     1981 atctacgcaa aagataactt cggcaatgaa aatggtttct tcggttaagc tgatgaaagc
     2041 tgaaaaagcc ataaacagtt ttttacctta tcaacaagct ttatctgaga ttttacgtaa
     2101 ttatttaagt aaccaaattc aaactttttc accttatact gatattcgag atgttcagca
     2161 tgtagctatt gtagtttttg cttcaaattc ttcgttgtgt ggcaattata attccaatat
     2221 gattaaacga atggagcaat ccattgttac tcatcgagat gtaagaaaag aagacatttt
     2281 aattttccct ataggaaaga aagtttcaca agcatgtaaa aaaaggaatt taaaaattgc
     2341 aggcgatttt gaagagattg ctgctcagcc tacttttgaa aatactgctc caat
//
LOCUS       DAOSAS010000187         2373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_29302_length_2373_cov_10.817947, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000187 DAOSAS010000000
VERSION     DAOSAS010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29302_length_2373_cov_10.817947"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1140
                     /locus_tag="PLR72_07160"
     CDS             82..1140
                     /locus_tag="PLR72_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOK37012.1"
                     /translation="MVDTLTNEAVELLQKMISIPSFSREEKELADFLEFYLQAKGYDV
                     FRNKNNIWLISPGFSPLRPTLLLNSHIDTVRPANDWTLNPFEPKIENGKLYGLGSNDA
                     GASVVSLLSAFIYLIQQNQSYNLIFAATAEEEISGENGIECLLEEFPPIDFAIVGEPT
                     GMKMAVAERGLMVLDCIARGKSAHAGREEGENAIYKAIEDIEWFRTYQFPKQSDFLGA
                     VKMTVTQIAGGTQHNVIPDTCTFVVDVRSNELYTNEEILEIIREHVHCEVEPRSIRLC
                     SSNVQLDHPIITRARELHLDLFGSPTLSDQALMPFPSVKIGPGDSARSHTANEFIELN
                     EIEKGIQIYIQLLNGLKW"
     gene            complement(1152..2108)
                     /locus_tag="PLR72_07165"
     CDS             complement(1152..2108)
                     /locus_tag="PLR72_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOK37013.1"
                     /translation="MDFVERSKEIFALEITELQKLANRLGPEINEAVEMIYNCKGKLV
                     IMGIGKTGIIGHKIASTLASTGTPTIFINAAEAVHGDLGMVSKNDIVLLISNSGSSSE
                     ILNVIFPLKNIGCTIIAMTGNPKSPLAKEASLILNVGVEREACPLGLAPTTSTTATLL
                     MGDALTICLMERRNFKAENFALYHPGGALGRQLLAQVKDEMVTDIPKVYTQTPFKDVI
                     YEVSNKRLGMTMVYNGNEEAVGIITDGDIRRAIQKFDNIKHLTAADFMTHGFKRITQE
                     ALLSEALEIMDENNITNLAVMETPDSDKVIGVITIHNIIDFK"
     gene            complement(2114..>2373)
                     /locus_tag="PLR72_07170"
     CDS             complement(2114..>2373)
                     /locus_tag="PLR72_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOK37014.1"
                     /translation="QGNREGGKLASAKEICEKEGITLDEVAYIGDDVNCVELLSNVGL
                     AACPADAQQAVKNIPGILQMKKKGGEGCIREFVELIMKYYC"
BASE COUNT          740 a          443 c          411 g          779 t
ORIGIN      
        1 taaaattgat aatcggttga aaacaattaa aaaatcgtaa aaatagatga tagttaagaa
       61 attttataga aattctttat tatggttgat actttaacaa acgaagcagt cgaactttta
      121 cagaaaatga tttctattcc ttctttcagc cgagaagaaa aagaattggc tgattttttg
      181 gaattttatc ttcaagcaaa aggttatgat gtttttcgca ataagaacaa tatatggtta
      241 atcagtccag gtttttctcc tttacgaccg actttactgc taaattcaca tattgacact
      301 gtaagacctg ccaacgattg gactttaaat cctttcgaac caaaaattga gaacggaaaa
      361 ttatatggtt taggaagcaa tgatgctgga gcaagcgtgg tgagtttgtt atctgccttc
      421 atatatttaa ttcaacaaaa tcaatcttac aatttaattt ttgctgctac agcagaagaa
      481 gaaatttctg gagagaatgg aattgaatgt ttgttagagg aatttcctcc tattgacttt
      541 gccattgtgg gtgaacccac tggaatgaaa atggctgtag ctgaaagagg gttaatggta
      601 cttgattgca tagcaagagg aaaatcggca catgccggta gagaagaagg cgaaaatgct
      661 atttacaaag ctatagagga tatagaatgg tttagaactt atcaattccc gaaacaatct
      721 gattttcttg gtgcagtgaa aatgacagtc acgcaaattg ctggtggtac acagcacaat
      781 gtaattcccg acacatgtac ttttgtggtg gatgtaagaa gtaatgaact ttatacgaat
      841 gaagaaattc tggaaataat tcgtgaacac gttcattgtg aagtagaacc gcgttcaatt
      901 cgtttgtgct caagtaacgt gcaattagat catcctatta ttaccagagc acgagaacta
      961 catcttgatt tgtttggctc ccctaccctt tccgaccagg ctttaatgcc tttcccaagt
     1021 gtcaaaattg gtccaggcga ttctgctcgc tcccacacag ccaatgaatt tattgaactg
     1081 aacgaaattg aaaaaggtat tcaaatttat attcagttac tgaatgggct caagtggtaa
     1141 ggaagtaatt tctatttaaa atcgataatg ttatgaatgg ttatcactcc gattacctta
     1201 tctgaatcag gtgtttccat aacagcaaga ttggtaatat tgttctcgtc cattatttct
     1261 aatgcttcac tcagcaaagc ttcttgagta atccgcttaa agccgtgtgt cataaaatcg
     1321 gctgctgtta agtgcttgat attatcaaat ttctgaatag cacgacgaat atcgccatcg
     1381 gtaataatac cgacagcttc ttcatttcca ttatacacca tagtcatacc taatcgtttg
     1441 ttgctcactt cgtaaatgac atccttgaag ggggtttgtg tataaacttt tggaatatca
     1501 gtaaccattt catcttttac ttgtgctaat aattgacgcc ccaatgctcc acctggatga
     1561 taaagtgcaa aattctcagc tttaaaattt cttcgctcca ttaagcaaat agttaaagca
     1621 tctcccatta ataaagtggc ggtggtagaa gttgtcggag caagtcccaa aggacaagct
     1681 tcacgttcta cacctacatt taatattagt gaagcttctt tggcaagagg cgattttggg
     1741 ttgccagtca tggcaataat cgtgcaacca atatttttta aaggaaaaat tacatttagg
     1801 atttcactgg aacttccact gtttgaaatg agtaaaacta tatcattttt acttaccatt
     1861 cccaaatccc catgaacagc ttccgcagca ttgataaaaa ttgttggtgt tcctgtcgaa
     1921 gctaatgttg atgctatttt atgtccaatg ataccggttt tcccaatacc cataatgact
     1981 aatttccctt tacagttgta aatcatttca actgcttcat ttatttcagg tcccaatcgg
     2041 ttagctaact tttgaagttc agtaatttcc agagcaaata tttctttact tcgttcaaca
     2101 aaatccataa tattcagcaa taatatttca taattagttc aacaaattct ctaatacaac
     2161 cttcaccacc ttttttcttc atttgtaata ttccaggtat gttttttact gcttgttgtg
     2221 catcagcagg gcaagctgct aatcctacat tagaaagtaa ttcgacacaa ttcacatcat
     2281 ccccaatata agcaacttcg tccagtgtaa ttccttcctt ttcacaaatt tcttttgctg
     2341 aagctaattt gccaccttct cgattacctt gat
//
LOCUS       DAOSAS010000188         2288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_30864_length_2288_cov_20.848186, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000188 DAOSAS010000000
VERSION     DAOSAS010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30864_length_2288_cov_20.848186"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2288
                     /locus_tag="PLR72_07175"
     CDS             <1..>2288
                     /locus_tag="PLR72_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOK37015.1"
                     /translation="FLISIFSVFSSYSASRYDLIPYPQELKPQSGEFILNKKTVILCP
                     FDQPQIVEIAQQFSDHFALVAGIKLPLRDYQTSSTNKNFILFNTGSLTNENPEAYTLK
                     ISTSSVQITGNTAAGVFYGLQTLYQLFPCEIYASIGSEKIKKWSVPCVEITDFPRFSY
                     RGLHLDVCRHFFPVDFIKKYIDLMAIYKFNYFHWHLTDDQGWRIEIKKYPRLTEIGSK
                     RNETLIGHYYESFPQQFDGKPYGGYYTQAEAKEIVAYAKKRGITVIPEIEMPGHAQAA
                     IAAYPFLSCTQDTTIKVATKWGIFKDVFCTRDTVFNFLEDVLSEIMEIFPSKYIHIGG
                     DECPKDRWKECPYCQSLIKKLNLKDEHQLQSYFTQRIEKFINEHGRQIIGWDEILEGG
                     LAPNATVMSWRGKIGGIEAARLSHDVIMTPTEYCYLDYYQADPEFEPLAIGGFVPLSK
                     VYNFEPIPDELTPAEAKYILGAQANLWTEYILDEKHVEYMAYPRATALSEVVWSNKES
                     KNWENFKERLSTHFQRFEKLGVNASHAFYEPQFNAEVTENNKLKITLNCDHPKAKIYF
                     SLNGKEPDQHYLKPLIIDSTTIVSATTFIDGKKVSRTLQKTFIVSKLTNLICQPDSNV
                     SYHQQSIFKLTDGKLGNIKTTSEWARLISNEDYLLNIDLQKNQIIQRFTMGILKAPAF
                     CGQIPSEIILYGSKDGINYQSLEEQKFSSSFTGKYEIIRPVFNFPPLEVRYLKIMLKN
                     PRFCIENLENEKNNSIFIDEIA"
BASE COUNT          783 a          358 c          378 g          769 t
ORIGIN      
        1 atttttaata agtatttttt ctgtatttag ttcatactca gcttctcgtt atgatttgat
       61 tccttatcca caagaactaa aacctcaatc tggtgaattt attttaaata agaaaaccgt
      121 cattctttgt ccttttgatc agccccaaat cgttgaaatt gctcaacaat tttctgatca
      181 ttttgctctg gttgctggca taaaattacc tttacgggat tatcaaacgt cttctacaaa
      241 taaaaatttc attttattta atacaggttc attaacaaat gaaaatcctg aagcttacac
      301 attaaaaatt tctacttctt cggttcaaat tacagggaac acagcggctg gcgtttttta
      361 cggcttacaa acattatacc agcttttccc gtgcgaaatt tatgcttcaa taggaagcga
      421 aaaaataaaa aaatggtcag ttccctgtgt tgaaattaca gattttccgc gtttcagcta
      481 cagaggactt catctcgatg tttgtcgtca tttctttcct gtagatttta taaaaaaata
      541 tatcgactta atggctattt ataagttcaa ctattttcat tggcatctta ctgatgatca
      601 gggttggcga atagaaataa aaaaatatcc acgccttaca gaaattggtt ctaaaagaaa
      661 tgaaacattg attgggcatt attatgagtc gtttccacaa caatttgatg gaaaacctta
      721 tggcggttat tatacccaag cagaagctaa agaaattgtt gcttatgcta aaaaacgagg
      781 cattactgta attcctgaaa tagaaatgcc ggggcatgct caagctgcta ttgctgctta
      841 tccttttttg tcgtgtaccc aagacacaac tattaaagtt gccactaaat ggggtatatt
      901 taaagatgta ttttgtacac gtgataccgt ttttaatttt ttagaagatg tattatctga
      961 aattatggag atttttcctt caaaatatat tcacattgga ggtgatgaat gtccgaaaga
     1021 tagatggaag gaatgtccct attgtcaatc gttgataaaa aagttgaatt tgaaagatga
     1081 gcatcaatta caaagttatt ttactcaacg gattgaaaaa tttattaatg aacatggtcg
     1141 acaaatcatt ggttgggatg aaattttaga aggtggttta gctcctaatg ctacagttat
     1201 gtcatggcga ggaaaaattg gtggcattga agctgctcgt ctttcgcacg atgtgattat
     1261 gacaccgacg gaatactgtt atttagatta ttatcaagct gatcccgaat ttgaaccact
     1321 cgctattggt ggttttgttc ctttatccaa agtatataat tttgaaccta ttccagatga
     1381 gttgactccc gcagaagcaa aatatatatt gggagctcaa gcaaatttgt ggacagaata
     1441 catccttgat gaaaaacatg tggaatatat ggcttatcca cgtgcaactg cattatcaga
     1501 agtagtatgg agcaacaagg aatcaaaaaa ttgggaaaat ttcaaagaac gtttgtcaac
     1561 tcattttcaa cgatttgaaa aacttggagt gaatgcctct catgcatttt atgaacctca
     1621 atttaatgcg gaagtaacgg aaaataataa attaaaaatt actttaaatt gtgatcatcc
     1681 aaaagcaaaa atttattttt cattaaatgg aaaagaaccc gatcagcatt acttaaaacc
     1741 gttaataatc gattcaacta ctattgtatc agctactact tttattgatg gtaagaaagt
     1801 aagcagaact ttacaaaaaa ctttcatagt tagcaaatta acaaatttaa tttgtcaacc
     1861 tgactccaat gtttcttatc atcaacaaag tatttttaaa ttaaccgatg gaaaattagg
     1921 aaatataaag accacttctg aatgggcgag attgatatca aatgaagatt atttattaaa
     1981 tatcgattta caaaagaatc aaattattca acgttttacg atgggaatat tgaaagcacc
     2041 tgctttttgt ggacaaattc cttctgaaat tattctttat gggtcaaaag atggaattaa
     2101 ttatcaatct cttgaagagc aaaaattctc atcttctttt actggaaaat atgaaattat
     2161 acgaccagta tttaattttc ctccacttga ggttcgttat ttaaaaataa tgttgaaaaa
     2221 tcctcgtttt tgcattgaaa atcttgaaaa tgaaaaaaat aattctattt tcatagatga
     2281 gattgctg
//
LOCUS       DAOSAS010000189         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_31079_length_2276_cov_11.883836, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000189 DAOSAS010000000
VERSION     DAOSAS010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31079_length_2276_cov_11.883836"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /gene="recJ"
                     /locus_tag="PLR72_07180"
     CDS             <1..1614
                     /gene="recJ"
                     /locus_tag="PLR72_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOK37016.1"
                     /translation="INNFEEARKFFRPDLNDLHDPFLMADMDKAVDRLTKAMIKNEKI
                     LIYGDYDVDGTTSVAMVYKFIKQYHSRIGFYIPDRYTEGYGISYEGINYAARNHFSLV
                     IALDCGIKDMEKIAYAAKLNIDFIICDHHTTGETLPSAIAVLDPKRNDCLYPYKHLSG
                     CGVGFKLLQAFSFKNDIAFSDFIPLLEYVTLSIASDIVPITGENRILAYYGLKQINTS
                     PSIGLKEIINVCNLSDKEITISDIVFKIGPRINSSGRIKSASEVVELLISNDVNFSRK
                     KSEILNEYNNDRKNLDKNITDEAIQMIANDKEYYKKKSIVVYQPHWHKGVLGIVASRL
                     TDEYYKPAIVLGRSNGMVSGSGRSVQGFDLYKAIEYCSDLLENFGGHMYAAGLSLKEK
                     NIPLFAAKFEEYVSENILEEQTYPQMDIDCILQFKDITPKFFRILKQFAPFGPENMKP
                     VFVSKKVFDYGTSRLVGKEQEHLKMELIDSSSENVMNGIAFGMSEYNTHLKSLQPLNI
                     CYTLEENNFNGNTTIQLIIKDMKVENENF"
     gene            complement(1636..>2276)
                     /locus_tag="PLR72_07185"
     CDS             complement(1636..>2276)
                     /locus_tag="PLR72_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOK37017.1"
                     /translation="FLYLFFILMDYERISTKWIELVPQKYRSIVWQIAQDIADGMNRY
                     FRGQALVAMCVGILHMIGLSIIGLPMSILMGLTIGILNLVPYLELLGIIPCFFLAVLK
                     SAETGQNFWLVVLSIAIVWLVVQAIQDLILVPKIMGKVTGLHPALILLSLSIWGALLG
                     LAGMIIALPITSLILSYYRRFIVLDERRTTHSGTFNEEWLSKRKDKDDSSSS"
BASE COUNT          792 a          386 c          389 g          709 t
ORIGIN      
        1 attaataatt ttgaagaagc acgaaagttt ttcagacctg acctgaacga tcttcacgat
       61 ccatttctca tggctgatat ggataaagcg gtcgacagat tgactaaagc tatgattaaa
      121 aatgaaaaaa tattaatcta tggtgattac gacgtggatg gcactacctc tgtggcaatg
      181 gtttataaat tcatcaaaca gtatcattcc agaataggtt tttatattcc agatcgttat
      241 acagaaggtt atggaatttc ttatgaagga attaattatg cagccagaaa tcatttcagc
      301 ctggtgattg ctctcgattg tggcatcaag gatatggaaa aaattgctta cgcagcaaaa
      361 ttgaacatag attttattat ttgtgatcat cacaccaccg gtgagacact tccctcagcc
      421 atagcagtgt tagaccccaa acgtaatgat tgtctttatc cgtataaaca tctttctggg
      481 tgcggtgtag gttttaagtt gcttcaagct ttttccttta aaaatgatat tgctttttca
      541 gactttattc ctttgttgga atatgttact ttaagcattg cttctgatat cgttcctatt
      601 actggtgaaa acagaatttt agcttattac ggtctgaaac agattaatac aagtccttcc
      661 attggtttaa aagaaattat caatgtatgc aatctgtcag ataaggaaat taccattagt
      721 gacatagttt ttaaaattgg tcctcgcatc aattccagcg gacggataaa atccgcttcg
      781 gaagttgtag aattgcttat atccaatgat gtgaattttt ccagaaaaaa aagtgaaatt
      841 ttgaacgagt acaataacga tcggaaaaat ttggataaaa acattacaga cgaagctatt
      901 caaatgattg ccaatgataa ggaatattat aagaaaaaat ctattgtagt ttatcaacct
      961 cattggcaca aaggagtatt aggaattgta gcttcacggc tcactgatga atattataaa
     1021 cctgctattg ttttaggacg atcaaatgga atggtttctg gttcgggtcg ttccgtgcag
     1081 ggttttgatc tttacaaagc catagaatat tgtagtgatt tacttgaaaa ttttggcgga
     1141 cacatgtatg cggctggttt atcgctaaaa gaaaaaaata taccgttgtt cgctgcaaaa
     1201 tttgaggaat acgtttcaga aaacattctt gaagagcaaa cctatcctca aatggatatt
     1261 gattgtattc ttcaatttaa agacattaca cccaaatttt ttcgtatctt aaaacaattt
     1321 gcaccttttg gtcccgaaaa tatgaaacct gtttttgttt caaaaaaagt ttttgattat
     1381 ggaacaagtc ggttggttgg caaagaacag gaacatttaa aaatggaatt aattgattca
     1441 agctccgaaa atgtgatgaa tggaattgct tttggtatga gtgaatataa cactcattta
     1501 aaatctcttc aacctctaaa tatttgttat actttggagg aaaataattt caatggcaat
     1561 actaccattc aactgataat taaagatatg aaggtcgaaa acgaaaattt ttgaaatgaa
     1621 aaataatatt atttttcaag aggaagaact gtcatcctta tcttttctct ttgaaagcca
     1681 ttcttcatta aaagttcccg agtgcgtagt tctcctttca tccaatacaa taaaacgtct
     1741 gtaatatgat aaaatgagag aagtaatggg caaagcaatt atcattccag ccaaccccaa
     1801 taaagcaccc cagatggaaa gcgacaataa aattaaagca ggatggagtc ctgttacttt
     1861 gcccattatt tttggaacca aaatcaaatc ttgtatggct tgcactacaa gccatacaat
     1921 agcaatactt aaaacaacaa gccaaaaatt ttgacctgtt tcagctgact ttaatactgc
     1981 cagaaaaaaa catggaataa tgcccagtaa ttccagatac ggaacaagat ttaaaatacc
     2041 aatagtcaag cccattaaaa tagacatcgg taaaccaata atacttagcc cgatcatgtg
     2101 caaaattcct acacacatag ccaccagcgc ttgccctcta aaatagcgat tcattccatc
     2161 tgcgatatct tgagctatct gccaaactat tgaacgatat ttttgaggaa ccagttcaat
     2221 ccatttggta gaaattcttt cgtaatccat taaaataaaa aacaaataaa gaaaaa
//
LOCUS       DAOSAS010000190         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_31235_length_2268_cov_19.319928, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000190 DAOSAS010000000
VERSION     DAOSAS010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31235_length_2268_cov_19.319928"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLR72_07190"
     CDS             complement(<1..242)
                     /locus_tag="PLR72_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOK37018.1"
                     /translation="MGKNINLAVLPGDGIGPEIIDQALKVTEAICNKFGHILTYEFGM
                     VGACAIDEVGEPYPQATHELCMQSDAVLFGAIGSPK"
     gene            complement(321..1865)
                     /locus_tag="PLR72_07195"
     CDS             complement(321..1865)
                     /locus_tag="PLR72_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOK37019.1"
                     /translation="MIEIMDTTLRDGEQTSGVSFSTQEKLSIARMLLEELCVDRIEIA
                     SARVSEGEFETVKRITEWAKRNGYLNKIEILGFVDGKNSLEWIQSAGGQVVNLLCKGS
                     LKHCTYQLKKTPEQHVADIEEVIFYAQQMGMAINIYLEDWSNGIKDSEEYVFYLMDEL
                     KNEKIQRFMLPDTLGILNPDEAFQFCKKMIDRYPHLHFDFHAHNDYDLAVANVYEAVK
                     AGVTGVHTTINGLGERAGNAPLSSVLAVLHDHLKIKTNLREDKVYSLSKLVATYSGIR
                     IPHNKPVIGENVFTQVAGIHADGDNKSNLYYNDLLPERFGRHREYALGKNSGKANILK
                     NLQELGIELDEEMMRKVTERVIELGDKKQFVTMDDLPYIVSDVLNSEPEEQSIKLLNY
                     SLSLAYGLRPVATVKMQINGEVFEQTAAGEGQYNAVSKAMWKIYKNLNKPTPELLDYE
                     VVIPPGGQTNAIVQTIITWKFNDKVFKTRGLDGDQTVAAIKATMRMLNLIEHGHIHKS
                     DYENLP"
     gene            complement(1948..>2268)
                     /locus_tag="PLR72_07200"
     CDS             complement(1948..>2268)
                     /locus_tag="PLR72_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOK37020.1"
                     /translation="YGFKVIVSSFFADIFRNNALNNGILPVVVSPEFLNEIFKCVFEN
                     PETTLTVDLENQTITNNATGRSETFEINSYKKECLLNGFDDIDYLLSKKDLIEAYEAK
                     KENF"
BASE COUNT          706 a          448 c          301 g          813 t
ORIGIN      
        1 tatttaggac ttccaattgc tccaaaaagt accgcatcag attgcatgca caattcatgt
       61 gtagcttgcg gataaggttc gcctacttca tctatggcac aagcaccaac cattccaaat
      121 tcataagtta atatatgtcc aaatttatta caaatagcct ctgtcacttt caaagcctga
      181 tcaataattt cgggtccaat tccatcacca ggcaaaactg ctaaattaat attctttccc
      241 attgttatta ttttttacgt ttttattacc aataaatatt cgacattaaa gataaattat
      301 ttttcctaaa aacatgttta ttacggcaaa ttttcataat cacttttatg aatatgacca
      361 tgttcaatca aattcaacat tcgcattgtc gcttttatgg ctgcaacagt ttggtctccg
      421 tctaatcctc tggttttgaa aactttatca ttaaatttcc aggtaataat agtttgaaca
      481 atcgcattgg tttgtccacc aggagggatt acaacttcgt aatcgagtaa ttcaggggta
      541 ggtttattca aatttttata aattttccac attgcctttg aaacggcatt gtattgccct
      601 tcgccagcgg ctgtttgttc aaaaacttct ccattaattt gcatcttaac ggttgccacc
      661 ggtcgaagtc cataagccaa tgataacgaa taatttaaca attttattga ttgttcttct
      721 ggctcactgt tcaaaacatc agagactatg taaggcaaat cgtccattgt gacaaattgt
      781 tttttatcac ctaattcaat gacgcgctct gtcacttttc tcatcatttc ctcatcaagc
      841 tcaataccca attcttgtaa atttttcaaa atatttgctt ttcctgaatt ctttcctaaa
      901 gcatattctc ggtgacgtcc aaaacgttcg ggtaataaat cattataata taaattactt
      961 ttattatctc catctgcatg aatgccagct acttgtgtga atacgttctc accaattacc
     1021 ggcttgttat gaggtattct tattcctgaa taagttgcta ccaatttgct gagcgaataa
     1081 actttgtctt cacgaagatt tgtttttatt ttcaaatgat catgaagtac ggctaaaaca
     1141 cttgaaagag gtgcatttcc tgccctttcg cccaagccat tgatggtagt atggacgcct
     1201 gttacaccgg cttttactgc ttcgtaaaca tttgcaactg ctaaatcata atcattatga
     1261 gcatgaaaat caaaatgcaa atggggataa cgatctatca tttttttaca aaattgaaaa
     1321 gcttcatcgg gattaagaat acctaacgta tcaggtaaca taaaacgttg aatcttttca
     1381 tttttcagtt catccatcaa ataaaataca tactcttcac tatcttttat tccatttgac
     1441 caatcttcca gatatatatt aattgccata cccatttgct gagcataaaa aataacttcc
     1501 tcaatatcag caacgtgttg ttcaggagtt ttctttaatt gataggtgca atgtttcaaa
     1561 gagcctttgc ataacaaatt aacaacttgt cctccagcac tttgaatcca ttctaaagaa
     1621 ttttttccat ctacaaaacc aagaatttca attttgttta gataaccatt cctttttgcc
     1681 cattcggtta ttctttttac agtttcaaat tccccttctg atacccgtgc tgaagcgatt
     1741 tcaattctat ctacacacaa ttcctctaac aacattcgag caatgcttaa tttttcctga
     1801 gtggaaaaag atactcctga tgtttgctca ccatcacgca gggtagtatc cattatttct
     1861 atcataacgt aattttttat attcttttaa tttattaaac aaatatgata gagattattc
     1921 gtttaaaacg attttttatt caagacttta aaaattttct tttttagctt cataagcttc
     1981 aattaaatct tttttgctta acaaataatc aatatcatca aatccgttca gaagacattc
     2041 ctttttatag gaatttattt caaaagtttc tgaccttcca gtagcattat tggtaattgt
     2101 ttgattttct aaatcaacgg tgagtgtcgt ttcaggattt tcaaaaacac atttaaaaat
     2161 ttcatttaaa aattctggtg aaaccactac gggcaaaatt ccattgttca aagcattatt
     2221 ccgaaaaata tcagcaaaaa agctcgaaac aatcacttta aaaccata
//
LOCUS       DAOSAS010000191         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_32752_length_2193_cov_8.971936, whole genome shotgun sequence.
ACCESSION   DAOSAS010000191 DAOSAS010000000
VERSION     DAOSAS010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32752_length_2193_cov_8.971936"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..364
                     /locus_tag="PLR72_07205"
     CDS             80..364
                     /locus_tag="PLR72_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK37021.1"
                     /translation="MKQVKLILAGLLIGLTSPILFLWLYLHFFFPLDLPFLEIIKKLS
                     PGILMGKLFLLSIVPDLFIGFIFYRFEHFKLASGVILGCIPYLIVSIFML"
     gene            739..1881
                     /gene="lpxB"
                     /locus_tag="PLR72_07210"
     CDS             739..1881
                     /gene="lpxB"
                     /locus_tag="PLR72_07210"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOK37022.1"
                     /translation="MKYFLIAGEASGDLHGAHLMHELKNLDAKAEFCFLGGDLMQAEG
                     GTMVKHYREMAFMGFIAVIKNGKKLMNNLKECKKTLRNFQPDILILIDYPSFNLRIAK
                     FAKKELQLPVCYYISPKVWAWKTFRIRQIKQYVDKMLVIFPFEVDFYAQYHYEVEYVG
                     NPTVDAVFSSLNQNQTFEEFCLFNEIEKKPVIALLAGSRKQEISTCLPKMVETASKFS
                     DFQVIIAGAPGIELEFYQVILKNKNIKVVFEQTYQLLLHAKAAIVNSGTATLETALIG
                     TPQVVVYSVAFGRLAYWAKNIFIKVKYISLVNLIVGREVVKELIAHLFTVENLAKELK
                     KLLYNEAYRTKIQEGYQEIKNILGDKGAAERAARKIIAFCEERKKN"
     gene            complement(2004..>2193)
                     /locus_tag="PLR72_07215"
     CDS             complement(2004..>2193)
                     /locus_tag="PLR72_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK37023.1"
                     /translation="GYDAQFGVRPLKRAIQKYVEDQLADIVLSGEIHPGNTILMDIDA
                     ENNTIKPKIIESTPILNN"
BASE COUNT          735 a          277 c          374 g          807 t
ORIGIN      
        1 gtagttccta caaaaattga tatgacggct tatgaagcca tagagctact taaacaatgg
       61 aattcagaga aataattcaa tgaagcaagt gaaacttatt ttagctggac ttttaattgg
      121 cttgacatct ccaattcttt ttttatggtt gtatttgcat ttcttttttc cacttgattt
      181 gcctttttta gaaatcatta aaaaattatc tcctggaatt ttaatgggga aattgttttt
      241 gctttccata gttccagatt tatttatcgg atttattttt tatcggtttg aacattttaa
      301 gctagcatca ggtgttattt taggttgcat accctattta atcgttagca tttttatgct
      361 ttaaattcat gtaaatatga attatttggt gaaaggacac actacttttt taattcctat
      421 gtttgcttaa agatatcctt ctacggaata aaattaattt ttttttaaaa aaaatttcac
      481 attttctatt tgtttgttca gctttgttcg gaatagtaat ttttcctttt cagttttttg
      541 ttaaaaaaac aaaaaacgtt ctatctttgt gcaatgtttt taaatgagaa ggggacttga
      601 attttaaaaa taaattgtaa tgttcacata ttcagaattt aataatttta gtttttctat
      661 tggttattaa ctgttcaaca gaaaaagtat ttttaagttt atctattcct atcgaaattg
      721 tttttataag atactgaaat gaaatatttt cttattgctg gtgaagcgtc gggcgattta
      781 catggagctc atttgatgca tgaattaaaa aatttggatg ccaaagctga attttgtttt
      841 ttaggaggcg accttatgca agctgaaggc ggaacgatgg taaaacatta ccgcgaaatg
      901 gcatttatgg gatttattgc tgtgataaaa aatgggaaaa aattgatgaa taatttaaaa
      961 gagtgcaaga aaactttaag aaattttcaa ccagatattc tcattttgat tgattatcca
     1021 agttttaatt taagaatagc gaaatttgcc aaaaaagaat tacagcttcc tgtttgttat
     1081 tacatttcgc ctaaagtatg ggcatggaaa acttttcgta taaggcaaat caagcaatat
     1141 gttgataaaa tgctggttat ttttcccttc gaagttgatt tttatgctca atatcattac
     1201 gaagtagaat atgttggaaa tccaactgtt gatgcagttt tttcaagctt gaaccaaaat
     1261 caaacttttg aagaattttg tctttttaat gaaatcgaga agaaacctgt tatagcatta
     1321 ctggcaggta gtcgcaaaca agaaataagt acttgtttac caaaaatggt agaaacagct
     1381 tctaaattta gtgattttca agttattatt gctggagcac ctggaattga attagaattt
     1441 tatcaagtta ttttgaagaa caaaaacata aaagttgttt ttgaacaaac atatcaatta
     1501 ttactacatg ctaaagctgc tattgtaaat tctggaacag ccacgcttga aaccgcatta
     1561 attggaacac cacaagtggt agtttattct gttgcttttg gccgtttagc ttattgggca
     1621 aaaaatattt ttattaaagt aaaatatatt tcattagtga atttaattgt tggcagagaa
     1681 gtggtaaaag agttgattgc ccatcttttt actgtagaga atttagcaaa ggaactgaaa
     1741 aaacttttat ataatgaagc atatcggacc aaaattcaag aaggttatca ggaaataaaa
     1801 aatatactgg gagacaaggg agcagcagaa cgagctgcga gaaaaattat tgctttttgt
     1861 gaggaacgta aaaagaacta attttaatcg aagtgggtaa agtttgaacg tttttttatt
     1921 tcttaagaga ctattaatga agcgaattaa tgaaaagata aggagctttg attatcaaag
     1981 ttccttatct ttttaactaa tggttagtta tttaaaattg gagtagattc tataattttt
     2041 ggttttatgg tattattttc ggcatcaata tccattaaaa tagtgtttcc tggatgaatt
     2101 tcgcccgata aaacaatatc agccaattgg tcttctacgt atttttgaat agcacgtttt
     2161 aatggacgta caccaaattg agcatcataa cct
//
LOCUS       DAOSAS010000192         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_37384_length_2002_cov_14.898819, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000192 DAOSAS010000000
VERSION     DAOSAS010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37384_length_2002_cov_14.898819"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..721
                     /locus_tag="PLR72_07220"
     CDS             2..721
                     /locus_tag="PLR72_07220"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOK37024.1"
                     /translation="MNNFLFFPETSSTNILLWQKLNEEKLPEGFVVYTDFQTAGKGQV
                     GNSWESAAGENILFSLLLYPQHIAIEEHFLISQITSIGIKKMLDNYISNVTIKWPNDI
                     YWKEKKLAGILVETSLQNRIIKSAVIGVGLNVNQKEFLSDAPNPVSLFQILGEKLDRQ
                     TLMNSIVQNILDIYYHWDNNKIRSCYQQFLYRKEDYHLFEANKEVFLAKIVDVLPSGQ
                     IELSTKEGVTKKFYFKEVKYL"
     gene            complement(735..950)
                     /locus_tag="PLR72_07225"
     CDS             complement(735..950)
                     /locus_tag="PLR72_07225"
                     /inference="COORDINATES: protein motif:HMM:NF027444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HOK37025.1"
                     /translation="MNLIFSILIFLLFFFLFGVIVLFGFFRSLFNFRGKGNKIFHHRK
                     REEKPIQRKEKIFDKNEGEYVDFEEIE"
     gene            complement(961..1908)
                     /gene="metF"
                     /locus_tag="PLR72_07230"
     CDS             complement(961..1908)
                     /gene="metF"
                     /locus_tag="PLR72_07230"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOK37026.1"
                     /translation="MSVVEKIMNSPSTAFSFELLPPLKGDGIESVYKTIDLLREFDPK
                     YINITTHRSEVVYKKNPQGLFEQVAIRYRPGTVAIAAAIQNKYKITVVPHVICSGYTK
                     EEIEYELIDLQFLGITDLLLLRGDKGKNDKEFIPTGHLHATDLQEQVNNFNKGFFLDG
                     TRMKSRVIPFSYGMACYPEMHEESPNFESELYYTKKKVDGGAEYLVTQMFFDNQKYYD
                     FVKKCREIGITVPIIPGIKPITLMNQLTVLPKIFHVEIPEEFATELRKCRTDKEATEV
                     GVEWCIKQAADLKAHGVPSIHFYSLMAAQSVKRVAKAVY"
BASE COUNT          681 a          333 c          306 g          682 t
ORIGIN      
        1 aatgaataat tttttatttt ttcctgaaac ttcttctacc aacatattat tatggcaaaa
       61 gttgaacgaa gaaaagctgc ccgaaggttt tgtcgtttac actgattttc aaactgctgg
      121 caaaggacaa gtaggcaatt cttgggaatc agcagcaggc gagaacattc tttttagttt
      181 acttttgtat cctcaacata ttgctatcga agaacatttt ttaatttcac aaatcaccag
      241 cattggcata aaaaaaatgt tagataatta tatttctaat gttaccatta aatggccaaa
      301 tgatatttat tggaaagaaa aaaagctggc cggtattctc gtggaaacat ccctgcagaa
      361 tagaataatt aaatcagcag tgattggagt cggtctgaat gtgaaccaaa aagaatttct
      421 gagcgacgca ccaaatcctg tgtcgctttt tcaaatttta ggcgaaaaac tggacaggca
      481 aacattgatg aattcaattg ttcagaacat tctcgatatt tactatcatt gggataataa
      541 taaaattcgt tcttgttacc aacaattttt atatcgaaaa gaagattatc atttatttga
      601 agctaataaa gaggtttttt tagcaaaaat tgtagatgtt ttaccaagtg gacaaattga
      661 attatcgacc aaagaaggag taacaaaaaa attttatttc aaggaagtca aatatttata
      721 atcaactttg atttttattc tatttcttcg aaatcaacgt attcgccttc gtttttatca
      781 aaaatttttt ctttgcgttg gataggtttc tcttctcttt tgcgatggtg aaatatttta
      841 tttcctttcc ccctgaaatt gaaaagagaa cgaaaaaaac caaataaaac gataacgcca
      901 aataaaaaga aaaacaataa aaagattaat attgagaaaa ttaaattcat gtcaattttt
      961 ttaataaaca gccttagcta cacgtttcac gctttgtgca gccattaagc tataaaaatg
     1021 aatacttggc acgccatgtg cttttaaatc agcagcttgc tttatacacc attctacacc
     1081 tacttcagtg gcttctttgt cagtgcggca tttgcgtaat tctgtagcaa attcttccgg
     1141 aatttctaca tgaaaaattt taggaagtac tgttaattga ttcattagtg taatgggttt
     1201 aattcctgga ataatgggaa ccgtgattcc tatttcacga cattttttta caaaatcata
     1261 atacttttga ttatcaaaaa acatttgggt taccaaatat tctgcacccc catcgacttt
     1321 tttctttgta taatacaatt ctgattcaaa atttggagat tcttcgtgca tttctggata
     1381 acaagccatt ccataagaga aaggtatcac acgtgatttc atgcgagtac catctaaaaa
     1441 aaatccttta ttaaagttgt ttacttgttc ttgtaaatcg gtagcatgca gatgacctgt
     1501 cggaataaat tccttgtcgt tttttccttt atcgccacgt aaaagcagta aatcggtgat
     1561 ccctaaaaat tgcaaatcta tcagttcata ttcaatttct tctttggtat atccactaca
     1621 aataacatga ggtacaacgg tgattttata tttattttga atagcagccg caatggctac
     1681 cgtaccagga cgataccgaa tggctacttg ctcaaaaagt ccttgcggat tttttttata
     1741 tactacttca ctgcgatgag tagtaatatt aatatatttt ggatcaaatt ctctcaataa
     1801 atcaatggtt ttataaaccg attcaatacc gtcacctttc aaaggtggga gcaactcaaa
     1861 agaaaaagcc gttgaaggag aattcattat tttttcaaca acactcataa attttgataa
     1921 ttacaataaa atgcttcttc gcaaattttt acttacaaaa gtacaaattt aattttgttc
     1981 taaaaatgaa ctttgaataa ga
//
LOCUS       DAOSAS010000193         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_39201_length_1938_cov_9.268189, whole genome shotgun sequence.
ACCESSION   DAOSAS010000193 DAOSAS010000000
VERSION     DAOSAS010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39201_length_1938_cov_9.268189"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..930
                     /gene="kduI"
                     /locus_tag="PLR72_07235"
     CDS             88..930
                     /gene="kduI"
                     /locus_tag="PLR72_07235"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOK37027.1"
                     /translation="MKTNYEIRYASHPQDVKNYDTSRLRKEFLVEKIFSKDEVNMVYS
                     LFDRIIIGGAMPVNEILPLETIEPLKSEYFLSRREFGTFNVGGPGKISVDGNVFELNY
                     KEALYLGAGNRKVYFESLDPSKPAKFYFNSTLAHRNYPDKKVTKANAVIAEMGSMESS
                     NHRNINKMLVSQVLDSCQLQMGMTELAPGSVWNTMPVHTHSRRMEAYFYFEVPEDEVV
                     CHFMGEPNETRHIWMKNEQAVISPQWSIHSAAATSNYTFIWGMAGENLDYGDQDFYKN
                     TDLR"
     gene            1034..1813
                     /locus_tag="PLR72_07240"
     CDS             1034..1813
                     /locus_tag="PLR72_07240"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HOK37028.1"
                     /translation="MNQFDLTGKIALITGASYGIGYAIAKAFYAAGATIVFNDINEEK
                     VNNGLASYQKDGIKAHGYVFDVTDEKSVNEAIEKITKEVGVIDILVNNAGIIKRIPAL
                     EMSAADFKQVVDVDLNGPFIVSKAVAPGMIAKGGGKIINICSMMSELGRETVSAYAAA
                     KGGLKMLTKNLACEWGQYNIQVNGIGPGYITTPQTAPLRVDGHPFNNFIISRTPAARW
                     GTPEDLQGPAVFLASSASNFVNGHILYVDGGILAYLGKQPS"
BASE COUNT          668 a          306 c          348 g          616 t
ORIGIN      
        1 ttgtcttctt ttcttgaatc aattcaggtc aacattaatt ttatttctaa cctttcgttt
       61 tatcaaaaaa tttaaaatat tactcatatg aaaacaaatt atgaaataag gtacgcctcc
      121 cacccgcaag atgtaaaaaa ttatgacact tccagattga ggaaagaatt cctcgtagaa
      181 aagatttttt caaaagatga agtgaatatg gtctattcat tgtttgaccg catcattata
      241 ggaggcgcca tgccagtgaa tgaaattctt cctctggaaa ccattgaacc gttaaagtcg
      301 gaatattttc tttcgagacg tgaattcgga acgtttaatg taggaggacc tggcaaaatt
      361 tccgttgatg gaaatgtttt tgaattaaac tataaagaag cactttattt aggtgcagga
      421 aataggaaag tttattttga aagtttagac ccttcaaaac cagcaaaatt ctatttcaac
      481 tctacccttg cacatagaaa ctatcctgac aaaaaagtta ctaaagcaaa tgctgtaata
      541 gctgaaatgg gttccatgga aagttcgaat cataggaaca ttaataaaat gcttgtatca
      601 caagtacttg attcttgcca gttacaaatg ggaatgacag aacttgctcc aggaagcgta
      661 tggaatacta tgccagttca tacccatagc agacgaatgg aagcatattt ttattttgaa
      721 gtacctgaag atgaagttgt ctgccatttt atgggtgagc ccaatgaaac ccgtcacatc
      781 tggatgaaaa atgaacaagc tgttatttca ccacaatggt caattcattc agcggctgct
      841 acaagcaact acacttttat ttggggaatg gcaggtgaaa atctggacta tggagatcag
      901 gatttttaca aaaatacgga tcttcgataa ttttctggaa ataaactttt ttgttttttt
      961 aaatttcaat ttttatcaat gaagaacaaa atcttgtaaa atatttaaat ttaatcttta
     1021 aaaaattaaa aaaatgaatc aatttgattt aacaggaaaa attgcactaa ttactggtgc
     1081 ttcttatgga ataggctatg ctattgcaaa agctttttac gccgcaggag ctaccattgt
     1141 atttaacgat attaatgaag aaaaagttaa caatggctta gcttcctatc agaaagatgg
     1201 aatcaaagca catggctatg tgtttgatgt aactgatgaa aaatctgtaa atgaagctat
     1261 tgaaaaaatt accaaagaag tcggtgttat tgatattttg gtaaacaatg ctggtattat
     1321 caaacgtatt ccagctcttg aaatgagtgc tgccgatttt aaacaagttg tagatgtaga
     1381 tttaaacgga ccttttatcg tttcaaaagc cgttgctcca ggaatgattg ccaaaggagg
     1441 aggaaaaatt attaatattt gctctatgat gagcgaatta ggaagggaaa cagtttctgc
     1501 ttatgcggct gctaaaggag gtctaaaaat gttaactaaa aatcttgctt gcgaatgggg
     1561 tcaatacaac attcaagtaa acggaatagg accaggttat attactactc cacagacagc
     1621 ccctttgcga gtagatggac atcctttcaa taattttatt atatcacgta ctccagcggc
     1681 acgttgggga acaccagaag atttgcaagg accagcagta tttcttgctt cgagtgcttc
     1741 caattttgtt aatggacaca tcttatacgt agatggtggt atattggcat atcttggtaa
     1801 acaaccttca taataaatta aaatatagcc aattaattaa aaaattaatt gacttttaaa
     1861 aatttgaata tgaaaaaaaa tcttttttat attttaattt cgttgattct tgcttcgtgt
     1921 tcttctctac caaaatca
//
LOCUS       DAOSAS010000194         1934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_39345_length_1934_cov_8.833954, whole genome shotgun sequence.
ACCESSION   DAOSAS010000194 DAOSAS010000000
VERSION     DAOSAS010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39345_length_1934_cov_8.833954"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLR72_07245"
     CDS             complement(<1..553)
                     /locus_tag="PLR72_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOK37029.1"
                     /translation="MKTKYLSILLLGLFFSFSVTFSAEKVKLVILHTNDTHSQIEPTE
                     KSPLPTSNRGGYARRMGVINQIREQEKNVLLLDAGDFWQGTPYFNFYHGRIEVDGMNR
                     MNYDAVTIGNHEFDNGLDTMAIVLKQAKFPIISSNYQFNNPLLDSIVKPYLILNKNGI
                     RIGIMALNIDPKGLILKINYKKLK"
     gene            complement(564..1325)
                     /locus_tag="PLR72_07250"
     CDS             complement(564..1325)
                     /locus_tag="PLR72_07250"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOK37030.1"
                     /translation="MLIKKKVLILLMICMLTSCNYKTWQKVAATSDKISIDSTTDMLI
                     DKEYEAFLQPIKQKIDAEMNRVIGETLVDLKSYAPESPLSNFCADVFRQIASEFLNEK
                     IEIGIVNLGGLRASIPTGKITVRQIFELMPFENELVILWLKGDKLEKLLQYFASIGGE
                     GISGIQMTIKNGKAESILVDNQPLDPEKTYIIATSDYLAEGNDNMIQLAEYEKRIDTG
                     LKIRDILINHIQAETQKGNKIQSKTDGRITIIQAF"
     gene            1688..1934
                     /locus_tag="PLR72_07255"
                     /pseudo
     CDS             1688..1934
                     /locus_tag="PLR72_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444069.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
BASE COUNT          611 a          299 c          306 g          718 t
ORIGIN      
        1 atttcaattt tttataatta atttttaaaa tcaatccttt ggggtcaata ttcagagcca
       61 tgataccgat acgaataccg tttttattta gtatcaagta aggtttaaca atgctgtcca
      121 gcaacggatt attaaattga taattagaac tgatgatggg aaattttgct tgtttcaaca
      181 caattgccat agtatcaagc ccattgtcaa attcgtggtt gcctatcgtt actgcatcgt
      241 aattcattct gttcattcca tcaacttcaa ttctgccgtg ataaaaatta aaatagggag
      301 ttccctgcca aaaatcgcca gcgtccaata ataaaacatt tttttcttgt tcgcgtattt
      361 gattgataac gcccattctg cgtgcataac cacctctgtt ggaagttggc agcggtgatt
      421 tttcagtggg ttcaatctgg ctatgcgtat cattggtatg aagaataacc agttttactt
      481 tttctgctga aaatgttaca gaaaaagaaa aaaataaacc gagtaataaa atggataaat
      541 atttggtttt cattttttat tgattaaaaa gcttgaatta tagtgattct accatctgtt
      601 tttgattgaa ttttattccc tttttgggtt tcagcttgga tatgattgat gagtatatct
      661 ctgattttta atcctgtatc aatacgtttt tcgtattcag caagttgaat catattgtca
      721 tttccttcgg ctaaataatc actggtggcg ataatatacg ttttttcagg atcaagtggt
      781 tgattgtcaa caagaatact ttctgctttt ccatttttta tggtcatttg tatgcctgaa
      841 attccttctc ctccgataga agcaaaatat tgcagtaatt tttccaattt atcgccttta
      901 agccagagaa taaccagttc attttcaaaa ggcatcaatt cgaaaatttg tcgtacggta
      961 atttttcctg ttggtatgct ggctcttaaa ccacccaaat taacaatgcc aatctcaatt
     1021 ttttcattta gaaattctga agctatttga cgaaatacat ctgcacaaaa gtttgaaaga
     1081 gggctttcag gagcataact tttcaaatcg accaatgttt ctccgatgac gcgattcatt
     1141 tctgcatcga ttttttgttt gataggctga aggaatgcct catattcttt atcaatcaac
     1201 atgtcagttg tactgtcaat agaaatttta tcagatgtgg ctgctacctt ttgccatgtt
     1261 ttgtagttgc aagaagttaa catgcaaatc atcagtagta ttaaaacttt ctttttgata
     1321 agcataaaaa ttaatttttt tgaattattg atggggataa aaatacaaaa aattaggtat
     1381 aactttagaa tttttaaaaa gttatcataa cactactcaa tgttctcgtg ccgagacaaa
     1441 ttcagatact aaaaatattc tttaaaaaaa cgcttaaggc tttacaagag ctaattttta
     1501 gtaaattttt ttaatgcaaa tattgtaaaa taattgaaac aacaaaaaat ttaattcttt
     1561 cctttaaaaa agctaatttt ttcttacttt tgaaaaatca ggctttaaaa gaagactcat
     1621 ttttaaaaca gttcttataa agagatttac ttttttaaag cttacataat taatttagaa
     1681 ttagtcaatg ttatttaaaa tttttaaaaa tattgataga aaaaattatc gtccgattta
     1741 tggaggatta gagattctta aatatttagg tcccggcttg ttggtaacgg ttggctttat
     1801 tgatccagga aactgggctt cgaatttagc tgctggagca caatatggtt atgccttgat
     1861 ttggatgatt actttttcca ccattatgct aatttttttc ggcacaatgt tgcacatctt
     1921 gtgaatagta acag
//
LOCUS       DAOSAS010000195         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_40155_length_1907_cov_11.541577, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000195 DAOSAS010000000
VERSION     DAOSAS010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40155_length_1907_cov_11.541577"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLR72_07260"
     CDS             <1..299
                     /locus_tag="PLR72_07260"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOK37031.1"
                     /translation="TVDSLLIFGLGALSFMIATAGGVLFAKIMNLFLKDGNKINPLIG
                     SAGVSAVPLSAHVSQSIGNSYDKNNHLLMHAMGPNVAGVIGSAVAAGILLSILS"
     gene            581..1117
                     /locus_tag="PLR72_07265"
     CDS             581..1117
                     /locus_tag="PLR72_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOK37032.1"
                     /translation="MDPFVIIKKFYQKETKLYHILITHSNQVKDKALGVTEKHPELQL
                     NKTFLEEAALLHDIGIFLCYAPDIYCYGTHQYIEHGYLGADLLRKEGLPLHALVAERH
                     TGIGFTKEIIIKEKLPLPHRDMVPTSLEEQVICYADKFFSKSKLTTSYTVEEIREYLK
                     KYGPSHVEKFDEWHARFK"
     gene            complement(1126..>1907)
                     /locus_tag="PLR72_07270"
     CDS             complement(1126..>1907)
                     /locus_tag="PLR72_07270"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOK37033.1"
                     /translation="EGRFLLQFSENYISENWYQGGNSNLAVLSILSGKWNYDDKSKIQ
                     WDNTAEWRVGFYSVFKDTTALRDININEDILKITSKFGYKASGNWFYSGSLDFATQFL
                     KNYQSISSNEMKAAFLTPIRLNVNFGMDYKYKKMFSLMFSPISLKYIYAKDTLNINPN
                     LFGIEKGKNRLIDFGNSLNAVLSYSPNHETQIDSKLTFYTNYKKVEIDWEIVGNFVIN
                     RFLSTRISLHPRYDNTIILAEGQRAKLQFKQLLSIGFSRKF"
BASE COUNT          669 a          266 c          293 g          679 t
ORIGIN      
        1 taactgtcga ttccttatta atctttggtt tgggagcttt atcatttatg attgccacag
       61 caggaggtgt attgtttgct aaaattatga atttatttct aaaagatggc aataaaatta
      121 atcctttaat tggatcagca ggagtatcgg ctgttcctct ttctgctcat gtttctcaat
      181 ctataggaaa ttcatacgat aaaaacaatc acttattgat gcatgcaatg ggaccaaatg
      241 tggcaggtgt aattggttca gcagtagctg ctggtatttt gttaagtatt ttaagttgac
      301 aattttatca ttatttctat ttcaagctca ttggtttcag tgggcttttg tattttaatc
      361 tgtcatttta aatgaagttt taaaaaattg attgtacaaa cgtttaggat tttttcaatg
      421 gaaattaaaa aagtcatatt tccatgcaat agttttattt ttatgaaatg atttgtttaa
      481 tcacaattaa aatctataat taaagaaaaa ttttttaatt gcttatttcc cggagagttt
      541 taaaatttaa gaaaattgaa aaatgaaaga taattcaaaa ttggatccat ttgttattat
      601 taaaaaattc tatcaaaaag agactaaact ttatcatatt ttaataacac atagcaatca
      661 agtaaaagat aaagctcttg gagtaactga aaaacatcct gaattacaat tgaataagac
      721 ttttttggag gaagctgcct tgttgcatga cattggcatt tttctttgtt atgcgcctga
      781 tatttattgt tatggaaccc atcaatatat tgaacatggt tatttaggag ccgacttatt
      841 aagaaaagaa ggattacctc ttcacgcttt ggtagccgaa agacatactg ggataggttt
      901 tactaaagaa atcattatta aagaaaaact tccacttcct catcgggata tggtgcctac
      961 ttcgttagaa gaacaagtaa tttgttatgc tgataaattt ttttcaaaaa gtaaattaac
     1021 cacttcctat actgtagaag aaataagaga atatttaaaa aaatatggtc cttctcatgt
     1081 agaaaaattc gatgaatggc atgctcgttt taaataatag atattttaaa atttacgtga
     1141 gaaaccaata ctcaataatt gtttgaattg aagtttagct cgttgaccct ctgctaaaat
     1201 aatagtatta tcataacgag gatgtaaaga aattcgagta gaaagaaaac gattgataac
     1261 aaaattaccc actatttccc aatcaatttc tactttttta tagttggtat aaaatgttaa
     1321 ttttgaatct atttgggttt catgattagg actgtaagat aaaacagcat ttaatgaatt
     1381 accaaaatca ataagacgat ttttaccttt ctcaatgcca aataaatttg gattaatatt
     1441 taaagtatct ttggcataaa tgtattttaa tgaaataggt gaaaacataa gtgagaacat
     1501 ttttttgtac ttataatcca ttccgaagtt aacatttaac ctgatggggg ttagaaaagc
     1561 tgctttcatt tcattagaac taattgattg atagttcttt aaaaattgag tagcaaaatc
     1621 aagcgaacca ctataaaacc aattaccact ggctttatat ccaaacttac tggtaatttt
     1681 taatatatct tcattgatat tgatgtcgcg aagagcggta gtatctttaa atacggaata
     1741 gaagccaaca cgccattcag cagtattatc ccattgaatt tttgacttgt catcataatt
     1801 ccattttccc gaaagaatac taagaacagc taaattacta ttaccgcctt gataccagtt
     1861 ttctgaaata tagttttctg aaaattgcaa caaaaatctt ccttcgt
//
LOCUS       DAOSAS010000196         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_40393_length_1900_cov_11.744715, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000196 DAOSAS010000000
VERSION     DAOSAS010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40393_length_1900_cov_11.744715"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1291)
                     /gene="metK"
                     /locus_tag="PLR72_07275"
     CDS             complement(11..1291)
                     /gene="metK"
                     /locus_tag="PLR72_07275"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOK37034.1"
                     /translation="MAYLFSSESVSEGHPDKVADRISDALVDNFLALDKNSKVACETL
                     VTTGQVIIAGEVKTSAYVDVQSVARQTINKIGYTKSEYMFDGNSCGVLSALHEQSPDI
                     ARGVEREDPMEQGAGDQGMMFGYACNETNNYMPIALDIAHTLVSELAKIRKEGKEMTY
                     LRPDAKSQVTIEYDDHNRPLRVHTIIVSTQHDEFIKAGNGKTQEEADIEMLSVIQKDI
                     KNILIPRVIIKDQQFERLFKDDYRLLVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKV
                     AHGGGAFSGKDPSKVDRSAAYAVRHIAKNMVAAGIADEVLIQIAYAIGVSKPINFYVN
                     TFGTAKIKRSDEEISKKIEKLFDLRPKAIEESLKLRNSIYEETAAYGHFGRTPIEVKK
                     YFQDGNGNKISHIVELFTWEKLDKIEEIRKAFQL"
BASE COUNT          610 a          350 c          265 g          675 t
ORIGIN      
        1 aaaaaagaaa tcaaagttga aaagcttttc ggatttcttc tattttgtcc aatttttccc
       61 aagtgaaaag ttctacaata tgtgaaattt tattgccatt tccgtcttga aaatattttt
      121 ttacttcaat gggggttctt ccaaaatgtc cataggctgc ggtttcttca taaattgaat
      181 tacgtagttt taaactttct tctatggctt ttggacgcaa atcaaagagc ttttcaattt
      241 ttttcgaaat ttcttcatcg ctccttttta ttttagctgt accaaaggta tttacgtaaa
      301 aattaatggg tttagacact ccaattgcat atgcaatctg tatcaatact tcatcggcta
      361 tgcctgcggc aaccatattt ttggcgatat gacggacagc ataggcagcc gaacgatcta
      421 cttttgatgg gtcttttcct gaaaaagctc ctcctccatg agctactttt cctccataag
      481 tgtccacaat aatttttctt cctgttaaac ctgtatctcc atgtggacct ccaataacaa
      541 atttcccagt aggattaact aacaaacgat aatcatcttt aaaaagccgt tcaaattgtt
      601 gatcttttat tattactctc ggaatcaaaa tatttttaat atctttttga ataacagaaa
      661 gcatttctat atcagcttct tcttgagttt ttccattacc agcttttata aattcatcat
      721 gttgtgtgga aacaataata gtatgaactc ttaaaggacg attgtgatca tcatattcaa
      781 tagttacctg agatttagca tcagggcgaa gataagtcat ttctttccct tctttacgaa
      841 tttttgccag ttcgcttact aatgtatgag caatatcgag agcgataggc atataattgt
      901 tggtttcatt gcaagcataa ccaaacatca ttccctggtc acctgctcct tgttccatcg
      961 gatcttcgcg ctcaacaccg cgagcaatat cgggtgattg ttcatgcaaa gccgacaata
     1021 cgccacaact gtttccatca aacatatatt cactttttgt ataacctatt ttattaatgg
     1081 tttgtcgcgc aacagattga acatctacat aagcacttgt tttgacttct cctgcaatga
     1141 ttacttgtcc tgtcgttaca agagtttcac acgctacttt tgaattttta tccaatgcta
     1201 aaaaattatc cactaatgca tcagaaattc gatccgctac tttgtcggga tgtccttcag
     1261 aaaccgattc agaactaaat aaatacgcca tgattttaat aatttaaaat tgttaaaatg
     1321 gtagttgaat tttaaattac aggcaggaag attcgaaaat acttataaat ggcaagcaga
     1381 aaatggtacg caattttagc atttttttct gtggttgcaa gcagctcaaa tctttccaca
     1441 cataatttga cagcacaaag ttacacaaag tattctaatt ttttatactt tgccaataat
     1501 aattttaaaa tgatttttta taaattccac cgaaacaata aaaggatgca taacttattg
     1561 attcatatgc aaattttcaa ataaatatag agcaagattc taaaaaaatt aatagtactt
     1621 taaaattaac ttttagatca aataatgact aaatgctaaa tcattgtcct ttttttcttt
     1681 gattcactac tcattttgta atcccagctc tttttcgtat cccctatccc ttctctttat
     1741 ggaaaaatat caaaaataaa aaacaatctt tttactcaaa ttgaaatata attaactata
     1801 ttttaagcaa aaaaaatcat agtttgggtt aaatttttat ttgcgaagaa aacaatctaa
     1861 aacgggctct catcaggtta ttgatttgca acgaaataag
//
LOCUS       DAOSAS010000197         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_41691_length_1860_cov_13.482548, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000197 DAOSAS010000000
VERSION     DAOSAS010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41691_length_1860_cov_13.482548"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..963
                     /locus_tag="PLR72_07280"
     CDS             46..963
                     /locus_tag="PLR72_07280"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOK37035.1"
                     /translation="MPASAKLTILGCGSALPTNKNFPSSFMFDIRDKQFMIDCGEGSQ
                     IRLRQMGLKTNRFGHIFISHLHGDHCFGLLGLISTFSLLHRTAELHIHAHPDLEKIME
                     TQMNYFCLGIPFKVIFHSFDPEINALIYEDRSIKVYTIPLKHKIPTCGFLFEEKPRQR
                     HILREMIDFYKIPISKIPKIKQGEDFVTEEGEVVPNEKLTVENIKPVRFAYCSDTMYD
                     EDIIPLIHGVDCLYHEATFMEEDAARAKKTFHSTARQAAEIAKKAEVKKLIIGHYSAR
                     YTDQSLVLKEALEVFDQAILGEDMQNYEF"
     gene            1018..1698
                     /gene="trmD"
                     /locus_tag="PLR72_07285"
     CDS             1018..1698
                     /gene="trmD"
                     /locus_tag="PLR72_07285"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOK37036.1"
                     /translation="MRIDIISAVPELLESPLNHSIVKRAKEKGIVEIYIHNLHDYTTD
                     KHRTVDDYAFGFSAGMVLKIEPIDRVISKLKSERNYDQIIYVTADGKKFTQQMANQLS
                     LYENLIILCGHYKGIDQRVREHLITMEISIGDFVLTGGELPAALITDAIVRLLPGAIS
                     DETSALLDSFQDNLLAPPVYTRPAEYKGWKVPDILLSGNEAKIQEWLYQQSVERTQEL
                     RPDLLNPE"
BASE COUNT          608 a          315 c          317 g          620 t
ORIGIN      
        1 acaattgtta aatgtaaatt tttcgttcct ttatttcttg atattatgcc tgcttctgca
       61 aaattaacaa ttttaggttg tggatcagcc ttaccaacaa ataagaattt tcccagctct
      121 tttatgttcg atatccgtga taaacaattt atgattgatt gtggcgaagg ttctcagatt
      181 cgtttacgac aaatgggact caaaaccaat cgtttcggac acatttttat ttctcatctt
      241 cacggtgacc attgctttgg attattagga ttaatttcaa ctttttcatt gcttcatcgc
      301 actgctgaat tacatattca tgcccatcct gacctggaaa aaattatgga aacgcaaatg
      361 aattattttt gtttgggcat accttttaaa gtaatattcc attcctttga tcctgaaatc
      421 aatgctttga tctacgaaga ccgttcaata aaggtttata ccattccttt gaaacataaa
      481 atacccactt gtggcttttt gtttgaagaa aaaccacggc aacgacacat tctccgtgaa
      541 atgatagatt tttacaaaat tcctatttca aaaattccaa aaataaaaca aggagaagat
      601 tttgtaacag aagagggtga agttgtcccg aatgagaagc taacagttga aaatataaag
      661 cctgtcagat ttgcttattg ttctgataca atgtatgatg aagacattat tccgcttatt
      721 cacggagtag attgtctcta tcacgaagca acttttatgg aagaagatgc ggctcgagca
      781 aaaaagactt ttcattccac tgctcgtcag gcggctgaaa ttgctaaaaa agcagaagtt
      841 aaaaaactga ttattggaca ttattcagct cgttatactg accaaagttt ggtactgaaa
      901 gaagcattgg aagtgtttga tcaagccatt ttgggcgaag atatgcagaa ctatgaattt
      961 taaaaataaa ttaacttctt actattattg attttttagc ttgaattatt taccattatg
     1021 cgtattgaca tcatttcagc ggttccagaa ttactcgaaa gtccactaaa tcactctatt
     1081 gtaaaaagag ccaaagaaaa aggaatcgta gaaatttaca ttcataattt gcatgattat
     1141 actactgata aacatcgaac ggtagatgac tatgcttttg gttttagtgc cgggatggtt
     1201 ctaaagatag aaccaattga tcgagtaatt tcaaaactta aaagtgaacg taactatgac
     1261 caaatcattt atgttactgc tgatggtaaa aaatttactc aacaaatggc aaatcaactt
     1321 tcactttatg agaatttgat catcctatgt ggacattata aaggtatcga tcaacgcgta
     1381 cgggagcatc taattactat ggaaattagc ataggtgatt ttgttttaac aggcggagaa
     1441 ttgccggctg ccctcattac agatgctatt gtacgattat tgccaggtgc aataagtgac
     1501 gaaacttcag ctttgcttga ttcttttcag gataatttgc tcgcaccacc agtttatacc
     1561 cgtccagcag aatacaaagg atggaaagtc cccgatattt tattatcagg aaatgaagcc
     1621 aaaattcagg aatggttgta tcaacaatcg gttgaacgta ctcaagaact tagacctgat
     1681 ttgttaaatc ctgaataaaa taattttatt ttccttccat tgacttatcg attatctttc
     1741 cataaaagtt tatatgttga acactaccgt tttatctgat ttgtaatcag acatttattg
     1801 ctttaaagtg gttaacttat ttcaagcaac aataagtgaa aaaatctttg tatttctttg
//
LOCUS       DAOSAS010000198         1825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_42828_length_1825_cov_13.388701, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000198 DAOSAS010000000
VERSION     DAOSAS010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42828_length_1825_cov_13.388701"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="PLR72_07290"
     CDS             complement(<1..1271)
                     /locus_tag="PLR72_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005803581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOK37037.1"
                     /translation="MIVNYSEQLHNVFQYSVQEAERLGNNYLGPEHLLLGILRNGKGK
                     AIDILNYSQINVSKIKQTIENQIRTDKEPDGEKIPVLKSTERIKKMMELEAKSLSAET
                     ADTEHLLLAILKEKDNLAGEILNEENLTYEIAKSYIEHSTDFQLNSSFQDDDDEEETF
                     HSQQKNISGSSKQSDTPALDTFGTDITKAAMENRLDPVVGRETEIERLAQILSRRKKN
                     NPVLIGDPGVGKSAIVEGLALRIVQHRVSRILFDKRIVALDMASIVAGTKYRGQFEER
                     IKAILNELAKNPNIILFIDEIHTIVGAGGTPGSLDAANMLKPALARGEIQCIGATTLD
                     EYRQSIEKDGALERRFQKIIVEPTTYDETLQILKNIQDRYEYHHNVRYTPEAIEACVR
                     LTDRYITDRCFPDKAIDALDEAGSRVHIGSIS"
     gene            1641..>1825
                     /locus_tag="PLR72_07295"
     CDS             1641..>1825
                     /locus_tag="PLR72_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK37038.1"
                     /translation="MSEKFAVSLRESSLARWTALGIVSFTMLCGYFIADAMSPLKPLL
                     ESQLLWTNIDYSIFTSA"
BASE COUNT          535 a          327 c          271 g          692 t
ORIGIN      
        1 gaaatagaac caatatgaac tctcgaacct gcttcgtcca aagcatcaat tgccttatcg
       61 gggaaacagc gatctgttat ataacgatca gtcagtctta cacaagcttc gattgcttcg
      121 ggcgtgtaac gaacgttatg atgatattca tacctatctt gaatgttttt caatatctgt
      181 aaagtttcat cataagtagt tggttctact ataatttttt gaaaacgacg ttctaacgca
      241 ccatcttttt caatactttg ccgatattca tccaacgtag tagcaccgat acattgtatt
      301 tcgccacgcg ccaatgctgg tttgagcatg tttgcagcat ctaatgatcc tggcgttcca
      361 cccgcaccaa caatggtatg aatttcatca ataaagagaa tgatattagg attttttgcc
      421 agttcattta aaatggcttt tatccgttct tcaaattgtc ctctgtattt tgttcctgct
      481 acaatagaag ccatatcaag agctacaatt cgcttatcaa acaaaattcg cgaaacacga
      541 tgttgaacaa ttcgtaaagc caatccttca actatggctg atttaccaac tcctggatcg
      601 ccaatcaaaa caggattatt tttctttctt ctacttaaaa tttgagccag acgttcgatt
      661 tcagtttctc ttcccaccac tggatctaac cgattttcca tagcagcttt tgtgatatct
      721 gttccaaaag tatccaatgc tggtgtatca ctttgttttg agctaccaga aatatttttt
      781 tgctggctat ggaaagtctc ttcctcatcg tcatcatctt ggaaacttga atttaactga
      841 aagtcagtag aatgttcaat atatgacttt gctatttcat aagtaagatt ttcttcgttt
      901 aatatttcac ctgccagatt atctttttct tttaaaatag ctaaaagcaa atgctctgta
      961 tcagcagttt cggctgacaa agatttggct tccaattcca tcattttttt tatacgttct
     1021 gtacttttta aaacaggaat tttttcacca tcaggttctt tatcagtacg tatttgattc
     1081 tcaatggttt gtttaatttt cgatacgttt atttgtgaat aatttagtat atcgatggct
     1141 ttaccttttc cattgcgaag aattcccaat aacaagtgtt caggcccaag ataattattt
     1201 cctaatcgtt ccgcttcttg gacgctgtat tgaaatacat tatgtaattg ttcagaataa
     1261 ttaactatca tgattgattt tctcctttgc tacaaatata attgaaataa tgttgagttt
     1321 gtatgtttct tgtatgtttt ttggttttta acttttgatt tttaatttta ttttcttttt
     1381 tgtttactca gaatcaagaa ttaaatttct tattttctat caaaaataat accgtaggca
     1441 tttatagtaa aatactgtaa ttttgtcact tcaaggtgaa atattgtcaa aaataaaaaa
     1501 ttttaaagat tatcataata agaaagttag ttattgaata aaagtaaaaa aacaattaaa
     1561 tttttttcat tagttttgta accctaaaaa cgtaaatatt ctctttttct tttagaattt
     1621 tcctaattaa aaacaatttt atgtcagaaa agtttgctgt ttcgcttcga gaatcttcct
     1681 tagccagatg gacggcttta ggcattgtat cgtttaccat gttatgcggt tactttatag
     1741 ccgatgcaat gagtcctcta aaacctttac ttgaaagtca gttgctgtgg accaatattg
     1801 attattccat ttttaccagt gccta
//
LOCUS       DAOSAS010000199         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_53718_length_1561_cov_6.743692, whole genome shotgun sequence.
ACCESSION   DAOSAS010000199 DAOSAS010000000
VERSION     DAOSAS010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53718_length_1561_cov_6.743692"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLR72_07300"
     CDS             <1..453
                     /locus_tag="PLR72_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOK37039.1"
                     /translation="GNIAITFTLALCTFLVVNISGTKEYWKGIFWPEVPAWLKVPIPL
                     MPVVEFIGIFTKPFALMIRLFANIVGGHSIVGGLVCLIFITVKLGTTINITMSAISVI
                     FTVFIDLIELLIAYIQAYIFTLLSALFIGLARVDSPHKISAVEATDQV"
     gene            531..779
                     /gene="atpE"
                     /locus_tag="PLR72_07305"
     CDS             531..779
                     /gene="atpE"
                     /locus_tag="PLR72_07305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOK37040.1"
                     /translation="MLLSILLQVATLDALAKLGAALAVGLTVIAAGSGIGKIGTSAME
                     SIARQPEMSSDIRMSMIIAAALIEGIALFAIVVCGFIL"
     gene            844..1344
                     /gene="atpF"
                     /locus_tag="PLR72_07310"
     CDS             844..1344
                     /gene="atpF"
                     /locus_tag="PLR72_07310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOK37041.1"
                     /translation="MSLLTPDGGLVFWMLLSFGIVVLILTKFGFPVILQMIEKRKSYI
                     EESLLEAEKARNELENVKLESKKIIDEAHQAHEKIMKEAAQLKESLLNDAREKASQEA
                     EKILQEARENIQKEKEAALEDIRRQVADMSIVISEKLLRTKLRNKEEQMQIIDRLIDE
                     MKINKS"
     gene            1353..>1561
                     /locus_tag="PLR72_07315"
     CDS             1353..>1561
                     /locus_tag="PLR72_07315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOK37042.1"
                     /translation="MKTGLIASRYAQALYEFASDYKMEDEIYKGMKILEKSFTLNREL
                     NAVLRNPLLKRELKKQLIITAYGGEV"
BASE COUNT          537 a          213 c          289 g          522 t
ORIGIN      
        1 ggaaatattg caattacttt tacattagca ctttgtactt tccttgtagt aaacatatct
       61 gggacaaaag aatattggaa aggaatattt tggccagaag tacctgcctg gctgaaagtt
      121 cctattcctt taatgccagt agtagaattt attgggattt tcaccaagcc ttttgctttg
      181 atgattcgtc tttttgctaa tattgtagga ggtcattcca tcgtaggagg attggtttgt
      241 ctgattttta taacagtgaa attaggaaca accataaata taacaatgtc agctatttca
      301 gtgattttta ccgtttttat tgatttgatt gaattgttaa ttgcttatat tcaagcttat
      361 atttttactt tgttatcagc gttatttatt ggattggcaa gagtggattc accacataaa
      421 atttcagctg tggaagccac tgaccaagta taaagttatt tgaaaaacaa aatttgttta
      481 tcatttatta ataaattttt caatttatca tttaaaaata aaaagttatg atgttactat
      541 cgattttatt acaagtagct actcttgatg ctttggcaaa attgggagcc gccttagcag
      601 taggtcttac agtcattgca gcaggttctg gaattggaaa aattggcacc tctgcaatgg
      661 aaagtattgc acgtcaacca gaaatgagtt ctgatattag aatgagtatg attattgctg
      721 ctgctctaat tgaaggaatt gctttgtttg caattgtagt ttgtggattt attctttaaa
      781 ttttttctaa aattaaacat ttcagtgtgt ttaataatgg tttattttta aataaagaat
      841 attatgtcac ttttaactcc tgatggtggt ttagttttct ggatgcttct ttcattcggg
      901 atagtggttc ttattctcac aaaatttggt ttccccgtca ttcttcaaat gattgagaaa
      961 cgaaagtcat acattgaaga atcattactt gaagctgaaa aagctcgtaa tgaattagaa
     1021 aatgtaaaat tggaaagtaa aaaaatcatt gatgaagctc atcaagcaca tgaaaaaatc
     1081 atgaaagaag ccgctcaatt aaaagaatca ttgttgaatg atgcacgaga aaaagcttca
     1141 caagaagcag aaaaaatctt gcaggaagca cgggagaaca ttcaaaagga aaaggaagcc
     1201 gctttagagg atattcgccg acaggtggct gatatgtcaa tagttatatc tgaaaaatta
     1261 ttgagaacta aattgaggaa caaagaagaa caaatgcaaa tcatcgaccg tttgattgac
     1321 gaaatgaaaa tcaataaatc ttaaactcta aaatgaaaac tggcttaatt gcttcgcgat
     1381 atgcacaggc attatatgaa ttcgcttccg actataaaat ggaagatgaa atctataaag
     1441 ggatgaagat tctggaaaaa tcattcactt taaatagaga attgaatgca gttcttagaa
     1501 atcctttgtt aaaaagagag ttaaaaaaac aacttattat taccgcttat ggtggagaag
     1561 t
//
LOCUS       DAOSAS010000200         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1903
            NODE_54623_length_1543_cov_10.434140, whole genome shotgun
            sequence.
ACCESSION   DAOSAS010000200 DAOSAS010000000
VERSION     DAOSAS010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863432
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:13:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,464
            CDSs (total)                      :: 1,428
            Genes (coding)                    :: 1,413
            CDSs (with protein)               :: 1,413
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54623_length_1543_cov_10.434140"
                     /isolate="AMR_MDS_1903"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..1520)
                     /locus_tag="PLR72_07320"
     CDS             complement(249..1520)
                     /locus_tag="PLR72_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOK37043.1"
                     /translation="MQPLAERLRPKTLDQFVGQEHLVGENAILRQMIESGHLASFILW
                     GPPGVGKTTLARIIANQLNRPFYSLSAINSGVKEVREIIEKAKNNKLFPSGNPILFID
                     EIHRFSKSQQDALLGAVENGTITLIGATTENPSFEIITPLLSRCQVYILKPLSADNLL
                     LLANRAITTDEILKKRKIEIDETEALLRFAGGDARKLFNIIELIVAAEPEDPVHITNQ
                     AVIECLQENPIAYDKNGELHYDIISAFIKSIRGSDPDAAIYWLARMIEGGEDPLFIAR
                     RLLIAASEDIGLANPNALLLANACFDAMQKIGWPEGRLILAETTIYLASSPKSNSAYL
                     AIDEAQNLVKKTGNLPVPLHLRNAPTDLMKKIGYGKDYLYPHDFPNHFVHQQYLPDEL
                     ADESIWKSQENPAEKKLADYLQQLWKNRYEK"
BASE COUNT          502 a          283 c          276 g          482 t
ORIGIN      
        1 aagaatacaa gagcaccaaa agaaagaaga aaaaccctgt tcgacgtagc ctcaagacta
       61 cgtcgaatta aaaataaaat ctccagattt tcaacattgc ttataataag cgaataaaat
      121 tggattctgc tgttatgttc cttgttatga aggttgtaag caaaataagg tttaaaaaaa
      181 taacgtcaca acaatatgct ttggaacgac atgagccggt aagccgaaaa aaagagaaag
      241 caagtttttt atttttcata tcgatttttc cacaattgtt gaagataatc tgccaacttt
      301 ttttctgctg gattttcctg agatttccaa atgctttcgt cggcaagttc atcgggtaaa
      361 tattgctgat gtacaaaatg attaggaaaa tcatgtggat acaagtaatc ttttccataa
      421 ccaatttttt tcatcaagtc ggtaggtgca tttcgtaaat gcaaagggac aggtaaattg
      481 ccagtttttt taactaaatt ttgagcttca tcaatggcaa gataagctga attgcttttg
      541 ggtgaagaag ccagataaat ggtcgtttcg gccaaaataa gtcttccttc gggccagcca
      601 attttttgca tagcatcgaa acaggcattg gctaataata aagcattggg gtttgccaga
      661 ccaatatctt cagaagcagc aatcaataat cgtcgagcaa taaaaagagg atcttcacca
      721 ccttctatca tgcgtgccaa ccaataaata gcagcatctg gatcgcttcc acgaatagat
      781 ttgataaatg ccgaaataat atcgtagtgc aattcaccgt ttttatcgta agcaatagga
      841 ttttcctgta aacattcaat aactgcttga tttgttatat gtacaggatc ttctggttct
      901 gcggcgacaa tcaattcaat gatattaaat aatttacgag catcaccgcc agcaaatctt
      961 aataaagctt ctgtttcatc aatttcaatt tttctctttt ttaatatttc atcagtagta
     1021 atagctcgat tagctaacag aagcaaatta tcagcactta acggctttaa aatatatact
     1081 tgacagcgag aaagaagagg agtaataatt tcaaacgaag gattttctgt ggtagctcca
     1141 atcaacgtta tggtgccatt ttcaaccgcc cctaacaagg catcttgttg cgatttgcta
     1201 aaacggtgaa tttcatcaat aaaaagaata gggtttcctg aaggaaataa tttattgttt
     1261 ttggcttttt cgatgatttc cctgacttct tttacacctg aattaatggc actcagtgaa
     1321 taaaaaggtc tattcaattg atttgcaatg attcgtgcga gcgtagtttt ccctactcca
     1381 ggcggtcccc ataaaataaa agaagcaagg tgccccgact cgatcatttg tcgcaaaata
     1441 gcattttcac ctaccaaatg ttcttgccct acaaattgat ccagtgtttt tggacgtaat
     1501 cgctctgcta aaggctgcat gaaaatctgt tttgaattac tat
//
