LOCUS       DAOSAT010000001        44432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_637_length_44432_cov_5.628772, whole genome shotgun sequence.
ACCESSION   DAOSAT010000001 DAOSAT010000000
VERSION     DAOSAT010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 44432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44432
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_637_length_44432_cov_5.628772"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLF90_00005"
     CDS             complement(<1..309)
                     /locus_tag="PLF90_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55700.1"
                     /translation="MTQKEKIFLFLIIFLVYLNSIVNPFIFDDIALVVNNGFIKNFKF
                     LKYYFTSNLFKCAGEEDLFYRPFQTLFYAIVYKISKGNTFGYHLLNILLHSGCAVLIY
                     L"
     gene            complement(316..2262)
                     /locus_tag="PLF90_00010"
     CDS             complement(316..2262)
                     /locus_tag="PLF90_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HOK55701.1"
                     /translation="MAEMEIKDFEPEISEKFEPGFNARTVLAILFIGFIMLPASIYFF
                     LITGGGLGGAAQWVTVILLLEIAKRSFIQLKKQELYIIYIIAGSVIGLEHLAALLIWR
                     QYLVQSPPAQFFGLTENMPNWVVPPAGSEALMKRTFLHKDWIKPIVIVILTSLLYRIN
                     MFSMGYFLFRITNDIEKLPFPMAPVASEGATALAESGAKKETWRWRVFSISAMIGIIF
                     GAFYVVIPAITGLLMATPLQLFPIPWVDFTTQLETVLPSGLFGFATDLGVILTGFILP
                     FPVIVGTFIGSILCKTFGNPLLHKIGIIKNWQPGMTAVPANVVTTMDFWINATIGTGI
                     VVGIIGIWRVLRAFSSFKNKDNEKQAVTIPPDRGDYPIPVAIGIWILSTILYLIITKT
                     LVPNFPVWIFIFFGIILTPFLSYTSARMFGITGVATGIAFPMLREATFILSGYKGVEI
                     WYAPIPYFNHGHTAQAFKQLELTRTRFTSWYKGEFTAFGITLIATFLFWQIIWKMNPI
                     PSSAYPYVQKIWPMNATFEALWTTSTLPTGEKWMIKAIKAKYILSSTFISFLIYGLFL
                     LLKIPIGTFYGMVGGITTLPHYAFPMFIGALLGRFYMAKKIGPNWRRYTPIILAGYSC
                     GMGLIGMVSIAVALIAKTIFQIIY"
     gene            complement(2275..6642)
                     /locus_tag="PLF90_00015"
     CDS             complement(2275..6642)
                     /locus_tag="PLF90_00015"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOK55702.1"
                     /translation="MRNKIIFFPFLFFLFSTFLFSQDTVSYLNFFSSLKSRFPGSEGH
                     KKAADFIENKFKEIGLKNVKRESFKSVVPVEKYAYIEYDGKKIDINCLWPNLVRTSTL
                     PPEGLEGKLIYAGKGNLKEVEGKDINGSIIVMDFDSGDNYLDFAMLGAKCFIFINRGE
                     ITRTEGEKKFLSVPLDVPRFYSDNEKILEIAKKEPTVKIYGRMDWETINDWNIYGFLE
                     GRDAKLKDETIVISSYYDSTSVVPSISPGATSACGISALIELAEYLVKNPPKRSIIFL
                     ATSSHFMNMKGIDAFIQKHCRNEKPFSERIKKEDKIKISLFIGLDLSGDSDKLGIWHN
                     STDFYYQRFFAPIGKKFMEYSEKVGKILGYTESVLVNGISPEKGVVWDTFLPERIRTD
                     GEFMILSGNPAVSFITVNDGRWRVDTPKDGFENLKLQNIIKQVNVLKFLLSYALNDEE
                     LLSKSELKMKDKMATLKAKIVTFNPKKSFVPNDPVKDAIVVPRDGTYKTNIGVRGHPL
                     EITNEKGIAEISRLTLSQSVYLNAFYINKENGEIIFAPDMGVNGNQQYPISFQMDTKE
                     KERMIVLFKCKEISLFGLIDPQYLIQINNINVFDLSNSAPDAYGYFLQYPSSIPFLWT
                     SYSEPVGTVFAKENSKIKILGETGPLGKRLLLLNSKETYTNKEWAEGLGFSVNEISAI
                     YNTPYQAARDMLILDNYRRYNFEKYGIKNERLFELQQKSKELLEKAEEALKEKRWYEF
                     LKFSRQSQSIEARAYPDVKNTANDVIKGIIFYFILLLPFAYFCERLFFSFSKIEKRIA
                     GIFGIFLAIYWIMRYVHPAFKLTNAPEVILLSFIVLTLSGVVISLIASKFEEQMQRIK
                     KETSKVYQTDVGRITASATAFSLGVANMKRRKTRTALTSITLILLTFTVLSFTSIKSY
                     LKFNQILRPNKPPYTGLLLRDRNWTPLQKISYDYTENEFSEKGVIAPRSWLILGSLGN
                     KTAIEIKCGNKSFLSTGILGMTPSEKEILPVESTIIYGNWFEKEDEDSVLISDKIAKE
                     LNIKKEDIGVAKVNIYGKEFLIKGIFDSKKFGDIKDLDDERLTPVDFSVLPEKEISQM
                     KTEKTSQIFSSQIQLRSFTHTEAENTVIIPFKKVMDLKGTLHSIAVKFTTSSENPKTL
                     VEEFITKLAGIVFAGFENKTIVYSSIGFTSFGGMSNILIPILIAALIVLNTMLGSVYE
                     RIREIGTYSAVGLAPVHIASLFFAESLVYAVLGAVSGYLLGQIVIKILMGAGLLKGLV
                     LNYSSLSAVIATIIIVITVLLSTLYPARKASQMAVPDVTRKWILPQPKGDEWEFEFPF
                     TVSEIEILGLATFLTEYFNTYQDVSLGNFYTSGAKLTYQKHPSGKNKYIVETNIWLAP
                     FDLGVSQEFKMIFEPLGQYNFYTINLFMKRASGESTDWKRLNRRFLDGIRKQFLIWRT
                     VSIEIKKEYEKQGKNNLGI"
     gene            complement(6632..7150)
                     /locus_tag="PLF90_00020"
     CDS             complement(6632..7150)
                     /locus_tag="PLF90_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55703.1"
                     /translation="MKWIFIPIIIFLSGCNTDLTEKKWREKVRMGDYYLANNNIDMAL
                     NCWMESLEFKKDLLTYEKIIASLIIKNDFEKGKKYTLEGLTHFKNNDNLIFNLGLLEF
                     YLKEYEESLETMEKLIEKNKYYPNAHYIKGLIYEKKGEFEKAKKEFIEEVNINPGSRK
                     AWQKIKEMKNEK"
     gene            complement(7330..8331)
                     /locus_tag="PLF90_00025"
     CDS             complement(7330..8331)
                     /locus_tag="PLF90_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pesticidal protein Cry7Aa"
                     /protein_id="HOK55704.1"
                     /translation="MKEIGKKIKRNLLYEGCFEWEKDGVFNPGVIVENNQVHIIYRAI
                     KCENYSRLGYLKLKTENEIEKCEYPVISPTEVYEKQGIEDPRITKIEDSYFITYTAYD
                     GKNARIAIAESKNLLNFKKLGIISPNITFLEALNISENDRYKDFWKKQVAERGENIII
                     WDKDGCLFPEKINGKYALLHRIEPDIQIVYFNDFFELKSKDFWEEYFRKIESKIVMKP
                     KYNWEEEKIGAGAPPIKTEYGWLLLYHGVDKNKIYRAGISLLDIENPQKEIARLPFPL
                     FEPDYDWEKNGNVPNVVFPTGAFIKDGILYIFYGCADKRIGVAEIELKEINKILKKV"
     gene            complement(8668..8752)
                     /locus_tag="PLF90_00030"
     tRNA            complement(8668..8752)
                     /locus_tag="PLF90_00030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8716..8718),aa:Leu,seq:gag)
     gene            complement(8777..9634)
                     /locus_tag="PLF90_00035"
     CDS             complement(8777..9634)
                     /locus_tag="PLF90_00035"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOK55705.1"
                     /translation="MENFFQDIWRKIENFWIKKGCLLWHPYNVEVGAGTLNPATFFGI
                     IGNKKWNVVYLEPSVRPADGRYGENPVRLYLHHQIQVIMKPPPFDIQKIYLKSLEYLG
                     ISTKEHDIRFIEDNWEAPTLGAWGVGWEVWIDGLEITQFTYMQQAGGIDLIPPAVEIT
                     YGLDRIVNFLQKEKNVFNLYWNKDVKFGDIQKEREREYSVYSFENADVNLLFEIFEKF
                     EKEAEKILNRELIYPAYDYILKLSHIFNLLDSRKAISVSERVRYIQRVRKLANRCALL
                     YIEKGKGII"
     gene            9690..>10320
                     /locus_tag="PLF90_00040"
     CDS             9690..>10320
                     /locus_tag="PLF90_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK55706.1"
                     /translation="MNNEKIKIVFVGVGAMGQCAHLKNYVLIPDCEVVAICEIREKLG
                     KKVAERYGIKNFYKGFDEMIEKEDFDGIVASQPFTRHYVILKEILKAKRPVFIEKPLA
                     SSVEIGEKILEIIEKSGTWVMVGYHKRSDPASIYVKKLIDEFKKTGEIGKLRYVRITM
                     PPGDWIAGGFKDLIITDEKLPENIEYDPKPKNMDEFEFKEYVDFVNYYIH"
     assembly_gap    10321..10330
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10331..10752
                     /locus_tag="PLF90_00045"
     CDS             <10331..10752
                     /locus_tag="PLF90_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK55707.1"
                     /translation="QINFMRFILGEDYKVKFADSSKVLMVVESNSGISGVIEMAPYNT
                     SIEWEEKVFICFEKGYIELSLPAPLASNRPGKVKIYKDIPSPLTIIPHFPWVSAMYQQ
                     AVNFVKAIKGEIKPPNDAKEAFKDLKVAEEYLTLFKK"
     gene            complement(10763..11728)
                     /locus_tag="PLF90_00050"
     CDS             complement(10763..11728)
                     /locus_tag="PLF90_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOK55708.1"
                     /translation="MKLGVFIVVFGGKSFDEALDKVKELGLEAVEIGTGNYPGDAFCK
                     VGELLENEGKLKEFKKAIEKRGLEISALSCHGNPLHPDKSIAESHRQVQRKTILLAEK
                     LGVGRIITFSGCPGDNENAKYPNWVTCPWPTDFSEILKWQWEKVVIPYWKEETAFARK
                     HNVKEICLEMHPGFVVYNPETLLKLREAAGEEIGANFDPSHLFWQGIDPIAAVRKLKG
                     AIYHVHAKDTKINPYTSSINGVLDTKTYLDEENRSWIFRTVGYGHGYEFWNDFISTLR
                     MVGYDGVLSIEHEDSLMSGEEGLKKAIEFLKNVLIKEPKPKAWWT"
     gene            complement(11779..13395)
                     /gene="argS"
                     /locus_tag="PLF90_00055"
     CDS             complement(11779..13395)
                     /gene="argS"
                     /locus_tag="PLF90_00055"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOK55709.1"
                     /translation="MRIDVKSTIENTLKKSIIDFKKDVNFDVLPVEKEEFGDFYTNIS
                     FRLADKENSPQKIAEVIKEKIYPDLKGIIKKIEVKNGFINFFIDEDIYRQHILKICQN
                     QKEYLKENIGNGKKVLIEFVSANPTGPLTIAHGRQAAYGESLSRILKFCNFDVTKEYY
                     INDAGRQMELLGESLKARYYQLKGVDYPVPEEGYLGEYLIDIARKIKETKDDKKFFTE
                     FAENEILKDIKNDLKNFGVSFDNWVRESQFIKNKEVENVLNILKEKNLIYEKDGSLWF
                     KSTYFGDDKDRVVVKSDGSYTYLATDIAYHKFKIDRGFEFLINIVGPDHHGYIPRLKA
                     SVASLGFNPDNLSVLIVQLTTLYRGKEKLSMSTRKGQFITLKELVNEIGPDASKFFFL
                     FRKIDSHLDFDLEVAKKKSNENPIYYLQYAYVRLKHLIEFGKEKGYKIDNFIPEIDLL
                     NKEEEIEIMKKIWKFNDVIRSVVKTYGVHLLAEYLLDVSKLFHSYYQKYRIVGGEKDL
                     TYARLFLVKSLLIIFELSLNLLNVSLPERM"
     gene            complement(13385..13732)
                     /gene="rbfA"
                     /locus_tag="PLF90_00060"
     CDS             complement(13385..13732)
                     /gene="rbfA"
                     /locus_tag="PLF90_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOK55710.1"
                     /translation="MKTRRQLKVESMLREEIENIIRRDVSDPRICFFTITYVHITGDL
                     KNVRVGISFMGDEKEKENAFRGILSAQKFIQHKLGQRISLKFTPSIEFELDERKEIRI
                     EQLLKEIKKENED"
     gene            13897..16077
                     /locus_tag="PLF90_00065"
     CDS             13897..16077
                     /locus_tag="PLF90_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOK55711.1"
                     /translation="MKWKNLENWVKGILIFGFTFYLFFSFISFDPFDPTFKFCQIGFP
                     INENIKNFGGKFGSYLTGLLFFLFGKSSYLIVIFMLFLGYNIIWGEKERLWKRITSIT
                     GLLLTLSVFLTVIRGSLGNFDGGLLGTVLTPVLKEYFGENGIFFILGLSGILFIGISY
                     RMYTEPFKVVFNDYFFNEDKIPVPEKVSLPQKQKRKIFETKEKIEIPQPKEEKLEEKK
                     TETEEEIKEEKEKKKIKKTSISRQKGVVYNLPPVDLLEIGNPTQKETKEDLEIYAQVI
                     EETLSEFDIEGEVVQINQGPRVTMFEVQLAPGIQVQKIFKIQDNIAMNLKTTTIRVVA
                     PLPNKSTVGIEVPNREISIVYLREIIESKEFQRSPSKLTLAIGKDIMGRPIVTDLKLM
                     PHLLIAGATGSGKTVCINSLITSILYKASPDEVKFIMIDPKMVELTLYNDLPYLLCPV
                     IVDIKKAVNALKWLIGEMTRRYKLFSEHKVRNIDVYNNIFKDNPLPYIVVVIDELADL
                     MAVAKNEIEHSIIRLAQLSRAAGIHLILATQRPSVNVITGVIKANLPCRISFQVPSKF
                     DSRTILDRIGAEKLLGRGDMLFLPPLSSSLIRIQGSFISDEEIEKVCEFIKKQRIPEY
                     NMEILERKTQEEETPEILQTEYDDEEVLYEQAKRIVITTKIASISMLQRRLRIGFNKA
                     ARLIERMEEEGIVGPYQEGKPRKVIINMEENQQNSEDNGFEEFK"
     gene            16055..16468
                     /locus_tag="PLF90_00070"
     CDS             16055..16468
                     /locus_tag="PLF90_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK55712.1"
                     /translation="MDLKSLSELIKKEREKKEIDYDRIYRDTKIVRKFIEYIEDGEWN
                     KFPGNFYRKAVLKKYLDYLGIEGINLDELFEEKKETCEEEIIEPKRERKDERNKEKNE
                     KKFYSDTKFYILIFLFLLFILLLLIGNLLFENLIK"
     gene            16465..17745
                     /gene="rimO"
                     /locus_tag="PLF90_00075"
     CDS             16465..17745
                     /gene="rimO"
                     /locus_tag="PLF90_00075"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOK55713.1"
                     /translation="MKIHLISLGCPKNLVDSEQLLAILGEEGFIITDSIDEADLVIIN
                     TCGFIREAVKESEENIKEIMRKNKELIIWGCLVQREKEKLLKFGKLKGIGGVGNPEEV
                     LKIIKEKKEGIIRIKESFFTCRKEFPRLITTFPYAYIKISDGCNNFCSYCLIPKLRGP
                     LRSRKIINILKEAENIEKLGFKEIILIAQDTTNYGEDLKDGSNLIKLLEELEKFNFEW
                     IRVMYMHPGHIKDELIEKIGGSKKICKYFDIPMQHVSQKILEKMNRPIIDYKKLIDKI
                     RSKVPSAAIRTNFIIGFPGETEEDFRKLLEFIEEVKIDRVGFFKYSREKGTVAYNFKE
                     QIEEKIKEERMKILIEKQKDVSEKILRKYTGKVLKVLIEKKDNDFYIGRTEYDAPEID
                     GLVYVKEKNLKTGEFYNVKIKKTDSYDLYGEKIG"
     gene            17742..18347
                     /locus_tag="PLF90_00080"
     CDS             17742..18347
                     /locus_tag="PLF90_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK55714.1"
                     /translation="MIIEKIIVGDVFTNCYIVGCEEEKICGIIDPGDSGEKIKERIEE
                     LGLKPVMIINTHGHFDHIGANHLFNLPVYIHKDDFEFLKDPEKNLSSFFSVPYVSKND
                     NIITVEEKDVIKIGKLYFEVIHTPGHTPGSICLKIENILFTGDTIFADGIGRTDFPEG
                     DEERLIKSIKEKILPLPDDFIIYPGHGKSAKLIEFKNLFFL"
     gene            18344..19114
                     /locus_tag="PLF90_00085"
     CDS             18344..19114
                     /locus_tag="PLF90_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK55715.1"
                     /translation="MKTEPIIGISSLHFIWHSLEEAFERCAVEFGFKFIEFSYSDLLK
                     EKDLLLMKEFKKKYNIETGLHIWDNIPEIGKEEAILKMKDYLNICSIAEISFLTAHLG
                     KYSEREIGLNIVFEVLKNVISLYEKKNIKICIENHYIDELGNSPQEFLYFFEKINSPS
                     LKFCFDYGHANLNGDIFEFIEKLYKFFGIVHVSDNNGDKDEHLHYGDGNIEWKEVLSL
                     TLKKGFFGPYIIEFGGKNEKIEKFKSFENDLKEIAKNL"
     gene            complement(19109..20527)
                     /locus_tag="PLF90_00090"
     CDS             complement(19109..20527)
                     /locus_tag="PLF90_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOK55716.1"
                     /translation="MKLLLLSDLHYKSDGISKIPERNVKFGIEFLKRIQKNLKEDIDV
                     VIIAGDLLDDGENPDSEKEYLEIKKIIDNFNAKKVIIAPGNHDKKIEEFFEIFGDYKY
                     FVYDNFLFYVFYDRYYEGEIAIRSQEEIENFKKIVRENPDKKIIAVQHNIIFPKIESD
                     YPFNLLDCEKIHKLYKENNVFLSISGHYHRGLDILNNEGVYYFTLPAVCEEPFKYFLV
                     EISSELKIEEKQLKNKITATDFHCHTEFGYCAEDVSMEKVIERCKLFGVEKVYFTEHA
                     GQLYLQPEDYWNFKFFYNPDILKKQRDEKKDRIKDYVEKFKSLNSNMAGIGLEVEIDK
                     NGKISLLEEDRKFFEILVGAVHYLPDEWCTSKSETEKKFLWSVEKLIENKIDILAHPF
                     RFFYRKNLEKPEYLYRDVVKMLKEGKVKAEINYHHNNPEYRFFRMCMEENVKIVFGSD
                     SHNLIEVGDFSKHIEFLEKIKL"
     gene            complement(20531..22063)
                     /gene="purH"
                     /locus_tag="PLF90_00095"
     CDS             complement(20531..22063)
                     /gene="purH"
                     /locus_tag="PLF90_00095"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOK55717.1"
                     /translation="MIKIERAIISVSDKTGIIELAKFLKKYNVEIISTGGTAKTLKEN
                     GIDVIEVSQFTGFPEILDGRVKTLHPKIYGGILSIKDKEDHLKQMEENKIQPIQLIIC
                     NLYPFEKTLKMGKSDEEIIENIDIGGPTMIRAGAKNYKYVCVLVKTEMYEEFIKEMEK
                     NNGTVSEEFSFKCAREVFRLTSRYDFIISSYFTDKIEKEILPSEMDIRLLKVQNLRYG
                     ENSHQKGAWYRFTGREFKREIFQGKELSFNNLLDMESAYNLVCQFEKCACVIIKHTNP
                     CGVATGSDLIDAYKKALQTDETSAFGGIAGFNKKVDKKLAEIFIERFYEIIIAPDYEE
                     EALEIFKKKPNLRVIKCPERIKYEYDFRTLADGFLVQTPDEIDYEKFEIVSEKKPTDE
                     EIEGLKFAWKVCKFVKSNAIVIATKDQTLGIGAGQMSRVDSTKIAVMKMKENFKKIPK
                     PIVLASDAFFPKPDSIEIAYQAGVTSIIQPGGSIEDKNVIEKCNKFGISMIFTGTRHF
                     KH"
     gene            complement(22079..23425)
                     /locus_tag="PLF90_00100"
     CDS             complement(22079..23425)
                     /locus_tag="PLF90_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK55718.1"
                     /translation="MEKDIIVIGGGPAGMASAWGCAKQGAKVLLIERYGFLGGMATAG
                     FVGSILGHYLNEKEPAVSGFLKFLIEKTYQMDGCEKWESAFRKYGISFDSEILKISSE
                     KLLLEEKVEILYHSFVSSVKIEDSLIKEIEVVNKSGKMKIKGKVFIDATGDADIAFYS
                     GFNYTKGRFFDGKMQSMGCMFKIAGIDEEKITEEVAKKAGNLLNELKEKGELIIYNTG
                     LGGKGSTTRKGERSFNVMRFQGDGTDVFDLTKGEIFIRDQIYKYWKFLKENVPGFESS
                     YISALPPNIGIRETRQIEGLYTLTEDDILKGRKFDSSIARGTYWIDIHCPLGRTKKVH
                     LCVKECQTEEYCIALDKFKDYLPAKNKLYPPKNDWFSIPYECLIPKNSKNLLVTGRCI
                     SADHKAMSSIRVMATCIATGHSAGIGAYIALDKNISVDKVNPLEVQKILEKQEGLY"
     gene            23481..24836
                     /gene="tilS"
                     /locus_tag="PLF90_00105"
     CDS             23481..24836
                     /gene="tilS"
                     /locus_tag="PLF90_00105"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOK55719.1"
                     /translation="MVNIFKRAEEFVSKNKLIEQGDRILLAVSGGPDSVFLFYFFIHL
                     LKKKKIDIKVAYIHHHLRKEADREVIFVKNLTNKYGVEFFREDIEIKEKKNIEKNLRE
                     KRYEKLYEIAEKTGCNKIATGHTLDDHIETFFMNLFRGSGTSGLCGIWQKSNISPGSE
                     IFIIRPLLCVEKRQIVKYLEENKIEYLIDTSNLSSNFFRNKIRNEVIPFLLKYRPSLK
                     KVILRTTEILKEDEIFLRKYTDAIFKDITMKEKNKIIIDVERFKKLDNSIKRRIASII
                     YRKIKNTPYINFSIIERLIKNIEMGENVYNSEIIEKILKGEEKEDKSFIYRIKVPGEI
                     EILDKFIIRSSFVPFSDKIFKNENKFTGYFDFSKIKGDIIVRNRKGGDRFMPLGLNKE
                     KKLSRFMIDKKIPEKEKDEILIFENNGKIIWVCGYEISEQFKINKNTEKILKIEVRSK
                     N"
     gene            24839..26140
                     /locus_tag="PLF90_00110"
     CDS             24839..26140
                     /locus_tag="PLF90_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HOK55720.1"
                     /translation="MENYIMERSTEKIFPDNYLPKEVRNKKLKDIVVLGSQYKKEESI
                     EIIYYLKCEGDIIEVAKKLAMDETTGKWIGDGKPTSLFKKCVADVDKVYIYDKGEGIV
                     FIRTPIINLSEEKDPLYQILMLACGGPVLEFVYYSDVALIDINLPEKILKKFPGPSFG
                     IEGIRKLTETPYPEPIVGTIVKPCAGMTEKEVAEKCYLAAKGGVKFIKDDEKMLGPSY
                     CDIKKKIKLVSNALKKVYEEIGNKCIYAPHLVERADRIKDTAKRYIDYGATGLMLNVV
                     LGHNFEVLKILREDKDINVPLYAHSGGRSALSTGNRRIDDAVIVKFIRLCGGDFFQHG
                     VFGVNDTHIASLDEDLLNHLIFVMRYNLKGINDTIPVAAGGLRVDNLKLNFERHYDEK
                     FGFGVALLAGSYLLGHQKGPYFGAKEFLSSVKNLIKFIRSK"
     gene            26188..26586
                     /locus_tag="PLF90_00115"
     CDS             26188..26586
                     /locus_tag="PLF90_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOK55721.1"
                     /translation="MCIIFFIAASSLPVLHRARNKAVIARTKAIINTIEAALSMYETD
                     FGDYPEGEGNGSKIIVELLQGPVDSDRWRGPYIRLKKDEIDREGNILDAWKMPIIYKY
                     PQTDYDNIPFLLLSAGPDRKFGTKDDIGNW"
     gene            26605..27525
                     /locus_tag="PLF90_00120"
     CDS             26605..27525
                     /locus_tag="PLF90_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOK55722.1"
                     /translation="MEELVKWQEKKRKKLYKLFGELPEGDYPVRVIKRIEEEREKYIL
                     EKLVLDLNGYELVPAYFVKPKNLKNRVPAIVFNHSHGGKYYLGKDEFIEGNSYMAVPP
                     YAEVITSLGFVGICIDHLNFGERSGRDELDLFKELIWKGRVLWGLMVFDSLKVVDYLM
                     TREEVDKERIATIGMSMGSTMAWYLSALDKRIKVCIDICCLTDFEELIKTRGISGHGI
                     YYFVPSLLKFFSTSDINSLIAPRPHLSVAGAFDRLTPLDGLKKIDRELKNIYKKFGKE
                     ENWQLKIYNVGHRETFQMRSDIIEFLLKHI"
     gene            27526..28248
                     /locus_tag="PLF90_00125"
     CDS             27526..28248
                     /locus_tag="PLF90_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HOK55723.1"
                     /translation="MDRFNLGIEVIKKAGEYLKEKINECKKLKENRYDVKLLQDIETE
                     KIIVENIKKIFPEDSFLCEERGIDRKNKDNLWIIDPLDGSLNFSRGIPHFCISIAFNG
                     KKEKFGIVYDFVREEIFTGIEGKGAYLNGKIINVSKTDKIEESILSIGFMSGEEEIEY
                     GLRILKEMGRKVKKIRMFGSASLDLCYLASGRIDLLIHLNLKKWDYEGGRIILKEAGG
                     IFKKEKINNIEVFKGTNRRLKI"
     gene            28248..29543
                     /locus_tag="PLF90_00130"
     CDS             28248..29543
                     /locus_tag="PLF90_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK55724.1"
                     /translation="MAKKSIRLEDVVLKPVTGLIERFNIFLKNMETKEKVSEVDGFVS
                     GLLDELKDIEKGILGFFAEILFMYHLSERMVSIENEISLVEILGERVKEFLKPDFIKI
                     YLKTSEGRISSAYSYPVDFEDETINSILEESLQKGESFLYEKKVDGKYYSILSIPLRT
                     TKEKYGFFITGRKRKKGFTPEEISLLIAGSTVVSFTISNLKLMQKMIVNERLVMIGHL
                     ISGLSHDLRNILTKFENGIYFIESGIEMKDMEDIKIGKDILKKNYIRLKEFVLSMVDY
                     SRDREVSFENVDLHSLIEDVISYFETTLKEKNIKILKEFDENLKLVKIDKHRIERMIS
                     NLIQNSIDAVKENEGIIKIKTKLMENSFQIIVEDNGCGIPEKNLDKIFDIFFTTKGSR
                     GTGFGLAIVEKVVKEHNGKIEVSSKVGEGTIFKITLPRL"
     gene            29553..30296
                     /locus_tag="PLF90_00135"
     CDS             29553..30296
                     /locus_tag="PLF90_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOK55725.1"
                     /translation="MKDIFILSVVQGICEWFPVSSSGHLFLLHRILGIGQDINLDIFL
                     HFSSLLALIIFFKKEILNVLKGFLSFDVKNENFKMSWYIISASIITGIIGFLIKDKEF
                     LENKNVVSAGFLITTILIFLSGKKGEKKVSFTSSLIIGFLQGIALIPGISRSGTTISV
                     AKILGIKNEDAFNFSFLLAVPAIIGVTLLKIDQIKNIRVDYIIIGFLTTFSVSIITLY
                     FLKKIMLKNRFQYFCIYTFMVFLFSLLIN"
     gene            complement(30289..31437)
                     /locus_tag="PLF90_00140"
     CDS             complement(30289..31437)
                     /locus_tag="PLF90_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55726.1"
                     /translation="MTSRERIYAVLRGGFPDRVPVSLYKINPFDNDSFWTKHKSFENL
                     LKKAQQIQDTFVFYRPKTGFFFSAPGSIDLKVEETHDTPISTTVKLTVETEYGPLTRI
                     ARTSNLSVHQWVVEPWIKREKDIDRFLSLPYIPYQPDFTDFYELEKKLGDKGVMVVAL
                     PDPLGVVGFLFAPGDFGKFALDYPKPIMQLLEKIYERLLDLYKFISFSISNAIIRIRG
                     AEYATPPNLPSEYFHDIKKVFTEYVYRFDKTLIEILKRGNFNYVCYHWHDGIEELLPI
                     ILKMPIDIIEPICNSFEIPNRILKVRKIVGDSITIMGGPMTDDFDFRTREEIRNMVKD
                     CLIQGARRGRFILIPSDIPESAPITPELEQSYIEFLEAGFNFGKYPIS"
     gene            complement(31437..32273)
                     /locus_tag="PLF90_00145"
     CDS             complement(31437..32273)
                     /locus_tag="PLF90_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008727393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK55727.1"
                     /translation="MKKSVNMWIIPGNVSFEDGLKMLKDAGFEGVEMNTTEGGILDLN
                     SSEKDVKKVAEKVRKTGLEIASLLVGQFWKYPLTSSDPEIRKKGEELLEKGIKIANYL
                     GTDAILVVPGVVASLSGQGEIVSYDIAYKRSQESIKKYVELAEKNNVFICVENVWNKF
                     LLSPLEMKRFVEEIGSEYVKVYFDVGNILLIGFPEMWIRILGKLIKRIHLKDFKLSIG
                     NINGFCDLLEGDVNWPEVIKALKEIGYTSFLTAELGPYKYYPETKIYTTSLAIDKILG
                     RK"
     gene            complement(32289..33410)
                     /locus_tag="PLF90_00150"
     CDS             complement(32289..33410)
                     /locus_tag="PLF90_00150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK55728.1"
                     /translation="MEIKNPSKQDYERIIRFLEDVYGHFYNLFPLSYPQAWKEKNTDF
                     NNILIIEEKNKLCSLVRIFPLTLIQKNIKIKFAGIGAVSTDYEERGKGYMSILLNEVI
                     KKMEKEGYPLSILWGDRHRYINFGYENSGKVINITITTRGFEKGKISSVRTKRYLGEK
                     EILLKIIDTYNRKNYRVERDFDYFNEIYKKMFISLYYSEENGKFSYVVINEQGSETKV
                     YEFGGEPDIILGILRYLSERFGKSRFYLEFPNFEEIPDIIFKTCSNWNISNTGMIKIV
                     SLKKTIETFLPLIEENFPEGEEIVFEIENKEKVGIKKENGEIKFIQEGKNVLKLREEE
                     MARLFFNLSDIGVNIDDKLRRICKTFLPFDLFFPALDHI"
     gene            33467..34558
                     /gene="lpxB"
                     /locus_tag="PLF90_00155"
     CDS             33467..34558
                     /gene="lpxB"
                     /locus_tag="PLF90_00155"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOK55729.1"
                     /translation="MKICILAGEKSGDNYGALLCRNFKKEKKDIFIFGTGGREMEKEG
                     AKLIKGFPFGQMGFSGVLKKIFPYISFMKKVIKEIEKEKPDIIIFIDNPGFNLEIAKR
                     LKRKYKTFYYIPPKIWAHNYRRIKILKKYIDFVIPIFHFEVDIYRKENIPYVYFGHPV
                     IDLIKTEVEKFEKKGYIIGILPGSRREELIYNLPAIKDVIKNLKNELDFDVWISSLNE
                     EFKEIIKKIFKDPDIEYKIEKDLYYLIENSDVILSVSGTVNLEVAYLKKPMVVFYRTS
                     FLNYFLCRFIVNVDFISPVNLILNEKVVPEYIQNFRIKDVVNDIINLIERKDIYKKQI
                     EGYERMIELLGEKNISEKICRFIIEGVKN"
     gene            34555..36345
                     /locus_tag="PLF90_00160"
     CDS             34555..36345
                     /locus_tag="PLF90_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK55730.1"
                     /translation="MIKEYLKIRNYIKIHWEYIVTGVILSILYIILNSISFTSIIPLI
                     DRILSGKKITLPENIPHFIGVKIEPIITKINSFPPLLVLKYLIAFIFLTFLLKGICFY
                     FSNYFFRFFSTKIQTDLRDKVYTKFLNLSLDFFTYTQTGELTTRVVYDIGLLNSVFEM
                     FFPNFIFPIFLVISYLSIVFIIDWQMSLVSIFIYPVILVPVLHWTRKLRNLGRIIQET
                     YGKIGNFINESIFGQKIIKAYNLEEETLKKFRKENENIFRTIMSINKRVLLIGPFTDF
                     CGAVASGLIIYYAGNKILAGTLTAGFLFLFFFALFSIISPLKETMQTYATIKHSSSVL
                     PRIFYILDFKSTVKDEGKEIFDGLKDKIEFKNVGFSYNSRTVLKNINLVVKKGDKIGI
                     VGKTGSGKSTLISLILRFFDPTEGEILIDGKNIKDFKLNSLRKHIGYVPQEPIIFYGT
                     IKDNITFGENNDKRFKEVIETVGLLKFINELPEKENTLLGEKGINLSGGQKQLISIAR
                     AIYKNPEILIFDEATASLDSESEKIVQKAIEKTMEGRTVFIVAHRLSTIKDTNKIIVL
                     EKGEIVEEGTHSQLYEKKGFYYKFLTLQQL"
     gene            complement(36347..38332)
                     /locus_tag="PLF90_00165"
     CDS             complement(36347..38332)
                     /locus_tag="PLF90_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOK55731.1"
                     /translation="MEFKNAEILYVSNEGDNSFSGRLKERSKKDGPFKTLQRAVDEII
                     KIKQKNGGILKKQVVITIKGGIYFLEKPVELTYEHSGTSDLPVIIMPYKNERVIISAG
                     EKLKNYKTEKIDGKEFFVFDVKGKKFRSLWVNDEYLKRARYPKNGYLKIAGISEEDEK
                     KEWNEGVYKVKIHNEDIKNWNNFKNSEVILMTRWVESRLPVYYVDINKGIIEFKKRTV
                     FKPQKDDLYYIENIFEFMDEKEWYFDFESEKLYFYPEEKTKNYEFIIPKFENVLIIKG
                     CPEKNEYVENIYFYNITFSHAEWYFPEKSLKGNESGGFAQAAIEIPGAVYCEGMRNCK
                     FENCEITNVGTYGFEFSKGCQNNKIINCKTHSIGAGGIKIGERITREEKNLKTYGNEI
                     KGCIIKDGGKIFHSAVGIWIGQSYRNIIEKNEIYDFYYTGISIGWTWGYGKSLAGGNI
                     VKFNNVHHIGKKTDGDGPILSDMGGIYTLGIQDGTLISNNIFHDIYGYWYGGWGIYFD
                     EGSTHIIAENNLVYNTTHGGFHQHYGKENIVRNNIFAFGRDQQIQITRPENHIRFTFE
                     RNIVIGNTEKWIYGGIDFNFMFNNNLYFRIDGKEIKIFDHGGKIYTFKDWQKKGMDTD
                     SILEDPLFYDIKNFNFKLKNNSPAFKIGFKEFELPER"
     gene            38526..39457
                     /locus_tag="PLF90_00170"
                     /pseudo
     CDS             38526..39457
                     /locus_tag="PLF90_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014041755.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            39460..40224
                     /locus_tag="PLF90_00175"
     CDS             39460..40224
                     /locus_tag="PLF90_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK55732.1"
                     /translation="MRILSIFKKEFKSYFISPVAYVVILIFNLISGFIFYALCAYYSI
                     LSTRFSGSPYPYFTLSPNEMIIKPLFFNMAITSLFVLPLLTMRLFSEERRLGTIELLF
                     TYPLKDSDIYWGKFLACFSIFTIMTLIPFLYIFILEKWVNLDKGIILNSFLGLLLLGG
                     SFISIGILISSLTESQIISAIITFGVSLLFWIIGWISEILPKYSNFFDFISIYNHYEN
                     FPKGILDTRDIIFYLSICFFFSFLTLRILISKKYRG"
     gene            40226..41566
                     /locus_tag="PLF90_00180"
     CDS             40226..41566
                     /locus_tag="PLF90_00180"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOK55733.1"
                     /translation="MVEKKFFYRINAFLMVLIFLGILIFVNLISEKNYKRIDLTKNKI
                     HSISPQTKKIIKNLKEPVEIIIFYREKIDEKTKELLEQYRSNSKFISYRFVDLDRDVL
                     LAKKYGITSYDTILIKTGENYEKIYSLGEKEITGAILKLTKKEKKKIYFTTGHGEKGI
                     DDKLSNFKKYLEEENYTVGETLILRDGIPDDCDILVICGPEVDFVDKEIEEIKKFIDS
                     GKRLLVFLDTGNFPNLKNLLGSYGIEIENDIIIDLAGRRFLGDALSPLVFDFPYHQIT
                     RDFSNSACVFSSVRSVKLKENLPSEIKGDVLARTSSASWAEKNMKEIEKGKVNYDEND
                     EKGPVSVAVLVEKEIKEKEGNKKACVAVFGDSDFVTDKMIGFLLNKDFAMNTVNYLAE
                     EDILISIREKKEENQPLILSEKAGKFLFFFPVVIIPVLIIISGSYITIRRKFLY"
     gene            41568..42896
                     /locus_tag="PLF90_00185"
     CDS             41568..42896
                     /locus_tag="PLF90_00185"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOK55734.1"
                     /translation="MKIFKKNLLLLIILLIVGSFYFYQVKIASKKKKNEEKKLFVFEK
                     EKIKGILIKNDDKEIEIVNENGLWKIKGKNYECDKNEIESLINKILSLEIERNIGTVD
                     DLSIYGLSSSEKQIEIQKDGEKFILYIGDETPSSSYLYTTKDKKEVLLVYKWDLDSIL
                     GKNIFDLRDKRIIPVDITKEDIEEIEVNKKNKTILLKRSGERWYIESPLKDIANREKI
                     ERVVGNIIDGEVKEFEELKTEKECGLERPESTIKLKTKDSNYFIYLGKKKENLYYAKN
                     SLKPYIFLADDKIVDDIPDEINELREKKLFDIDVSEVEEFSIIKKDKELKFKKEKDSY
                     FLEGEKTKKISKDKVEEFLHDLKYLEIKDFIEYSEKNLKDFQLSPPLIKIIVYTEGTK
                     TEVHFGKKTENYIYCYSPERKSIFTIPSSDYSRIDKDEGFFVEEKKRKNE"
     gene            42889..44061
                     /locus_tag="PLF90_00190"
     CDS             42889..44061
                     /locus_tag="PLF90_00190"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOK55735.1"
                     /translation="MNKGIKTLIFLVLIFIIFFSFFKISKKQSIPEITIIGGEIKEGD
                     IIGNIFKTNGLSDYEAGKITEKLREIFDVRKSKIGDRWELHFSLKGDFLKFKYFSKPF
                     NFYIVEKKQDTGDFISYTEEIEKFKKTFTKTGFINNSLYESMINSNINPEIIIQFAEV
                     FESKIDFFTECQKDDKFSIIWESWADKDGTILKDIRVVAGKYETRNQKYYAFYFENGN
                     FSGFFDENGKGLEGSFLKAPLSYRRITSFFSYSRFHPIYKVYRPHLGIDYSAPTGTPV
                     SSIGNGSVIFAGWTNNGYGKCVKIKHPNGYISYYGHLSRIEKGIKKGVKVKKGEVIGY
                     VGMTGIATGPHLDFRVQKDGKFINFLKLKFVPEKNIPKEYMDEFENLKKRYMELLG"
BASE COUNT        15727 a         6409 c         6532 g        15754 t
ORIGIN      
        1 taaataaatt aaaacagcac atcctgaatg aagaagtata ttaagtaagt gatagccaaa
       61 agtattccct tttgagattt tgtaaacaat tgcataaaat aatgtctgga atggtctgta
      121 aaaaaggtcc tcttcacctg cacatttaaa taaatttgaa gtaaaataat atttcaaaaa
      181 tttaaaattt ttgataaatc cattattaac aacaagagca atatcatcaa aaataaacgg
      241 gtttacaatt gaattaagat agacaagaaa aataattaaa aaaaggaaaa ttttttcttt
      301 ttgagtcatc aaattttaat aaattatctg gaatattgtt tttgcaatta aagcaactgc
      361 aattgaaacc ataccaatta atcccattcc acaggaatat cctgcaagta ttatcggtgt
      421 atatcttctc cagttaggtc ctattttttt tgccatataa aatcttccaa gaagtgctcc
      481 aataaacatt ggaaatgcat aatgaggtag agttgtaatt ccaccaacca ttccataaaa
      541 tgtcccaatt gggattttga gtaataaaaa tagtccataa ataaggaaac ttataaaagt
      601 tgaacttaat atatattttg cctttattgc ttttatcatc cacttttcac ctgttggtaa
      661 tgtacttgtt gtccaaagtg cttcaaatgt ggcattcata ggccagattt tctgaacata
      721 tggatatgca gaggaaggta ttggattcat tttccatatt atctgccaga ataaaaatgt
      781 tgcaataaga gttataccaa aagcagtaaa ttctccttta taccatgaag taaatcttgt
      841 tcttgtaagt tcaagttgtt taaatgcctg tgcagtatgt ccatgattaa aatatggaat
      901 cggagcatac catatttcaa ctcctttata tccacttaat ataaatgttg cttctcttaa
      961 cataggaaat gcaatgccag tagcaactcc tgttattcca aacattcttg cagatgtata
     1021 acttaaaaat ggagtaagaa ttataccaaa gaaaatgaaa atccatacag gaaaatttgg
     1081 aacaagggtt tttgttatta taagatataa aatagttgat aatatccata ttccaattgc
     1141 tactggtata ggatagtcac ctctatcagg aggaattgta actgcctgtt tttcattatc
     1201 tttgttttta aaacttgaaa atgctcttaa tactctccat attcctataa taccaacgac
     1261 tattcctgtt ccaatagttg catttatcca gaaatccatt gtagttacaa catttgctgg
     1321 aactgctgtc atacctggtt gccagttttt tattatacca attttatgaa gtaatggatt
     1381 cccaaaagtt ttacataaaa ttgaaccaat aaaagttcca acaataacag ggaaaggtaa
     1441 aataaatcca gtaagaataa ctccaaggtc tgttgcaaat ccaaaaagtc cagatggtaa
     1501 aactgtttct aattgtgttg taaaatcaac ccatggaatt ggaaaaagtt gaaggggagt
     1561 tgccattaaa agacctgtta ttgcagggat gacaacataa aaagcaccaa aaataatacc
     1621 aatcattgca gaaatactga aaacacgcca tctccatgtt tctttttttg ctccactttc
     1681 agcaagtgct gttgctcctt ctgaagcaac aggagccatt gggaaaggta atttttcaat
     1741 atcgtttgta atacgaaata ggaaataacc cattgaaaac atatttattc tgtaaagtaa
     1801 acttgttaaa ataactatta ctattggttt tatccagtcc ttatgtaaaa atgttctttt
     1861 catcaatgct tcagaaccag caggaggaac aacccagttg ggcatatttt cagttaatcc
     1921 aaaaaattgg gcaggaggtg attgaacaag atattgcctc catatcaaaa gggctgcaag
     1981 gtgttcaagt cctataacag aaccagcgat tatataaatt atgtaaagtt cctgtttttt
     2041 caactgaata aatgaccttt ttgctatctc aagaagaaga ataaccgtta cccattgagc
     2101 agcaccccca agtccaccac ctgtaatgag aaagaaataa attgaggcgg gaagcattat
     2161 aaaaccaatg aataaaatag ccagaactgt tcttgcatta aaacctggtt caaatttttc
     2221 tgatatttct ggttcaaagt cctttatttc catttctgcc atttttcccc ctatttaaat
     2281 tccaagatta tttttccctt gtttttcata ttctttttta atttctatac ttactgttct
     2341 ccatatcaaa aattgttttc taattccatc aaggaatctt ctattaagtc gtttccagtc
     2401 agttgattct ccacttgctc ttttcataaa aagatttatt gtatagaaat tatattgccc
     2461 aagtggttca aaaatcattt tgaattcctg acttactcca aggtcaaaag gagcaagcca
     2521 tatatttgtc tcaacaatat atttattttt tccagaagga tgtttttgat aggttaattt
     2581 agccccgctt gtgtaaaaat tgccaagaga aacatcttga taagtattga aatattctgt
     2641 taaaaatgta gcaagcccaa gaatttcaat ttcacttaca gtaaaaggaa attcaaattc
     2701 ccattcatca ccttttggtt gtggtaatat ccattttctt gtaacatcag gaacagccat
     2761 ttgagatgct ttccttgcag gatataaagt tgaaaggaga actgtaatta caattattat
     2821 tgtagcaata acagcagaaa gagaagaata gtttaaaaca agtcctttta ataaaccagc
     2881 acccatcagt atttttataa caatctgacc caatagatag ccacttacag cacctaaaac
     2941 agcataaaca agagattcag caaaaaataa agaagcaata tgtacaggag caagtccaac
     3001 agcactgtaa gtcccaattt ctctaattct ttcataaaca gaaccaagca ttgtatttaa
     3061 aacaatcaat gctgctatta aaattggtat aaggatattg ctcataccac caaatgaagt
     3121 gaaacctata ctactgtaaa caattgtttt attttcaaaa ccagcaaata caattccagc
     3181 aagttttgtt ataaattcct caacaagtgt ttttggattt tcagatgaag ttgtaaattt
     3241 aacagcaatt gaatgtaaag taccttttaa atccataacc tttttaaatg gtataataac
     3301 agtattttct gcttctgtat gagtaaaaga ccttaactga atctgggatg aaaatatttg
     3361 ggatgttttt tctgtcttca tctgcgagat ttctttttca ggaagaacag aaaaatcaac
     3421 aggagtaagt cgttcatcat caaggtcttt aatatctcca aattttttac tatcaaatat
     3481 cccttttatc aaaaattctt ttccataaat atttacttta gcaactccta tatcttcttt
     3541 ttttatattt aattcttttg ctattttatc cgaaattaaa acactatcct catcttcttt
     3601 ttcaaaccag tttccataaa ttattgtact ttcaacaggt aaaatttctt tttcagaagg
     3661 ggtcatccca aggattcctg tagaaaggaa tgatttattt ccacatttta tttctattgc
     3721 agttttattt cccaaacttc caagtattaa ccaacttctc ggagctatta ctcctttttc
     3781 tgaaaattca ttttctgtat agtcatagga tattttttgc aatggagtcc agtttctgtc
     3841 ccttaaaagt aatccggtat atggtggttt attgggtctt aaaatctgat tgaattttaa
     3901 atatgatttt attgatgtaa atgaaagaac tgtaaatgta agaaggatta atgttattga
     3961 ggttaaagca gttcttgttt ttctcctttt catatttgca acaccgagag aaaaagcagt
     4021 tgcacttgct gttattcttc caacatctgt ttgataaacc tttgatgtct cctttttaat
     4081 tctctgcatt tgttcttcaa atttagaagc gataagtgat ataacaactc ctgaaagtgt
     4141 gagaacaata aaagaaagaa gtataacttc aggggcattt gtaagtttaa aggcaggatg
     4201 aacatatctc attatccagt aaatagcgag aaatattcca aaaattcctg ctattctttt
     4261 ttctattttt gagaaactga aaaataatct ttcacagaaa taggcaaaag gtaataaaag
     4321 tatgaaataa aaaattattc ccttaattac atcatttgct gtatttttta catcaggata
     4381 tgctcttgct tctatactct gggactgtct tgaaaatttt aaaaactcat accacctttt
     4441 ttcttttaat gcttcttctg ctttttcaag taattctttt gatttttgct gtaattcaaa
     4501 aagtcgttca tttttaattc cgtatttttc aaagttatat cttctgtaat tgtcaagtat
     4561 aagcatatct cttgccgcct gataaggtgt attatatatt gctgaaattt cgtttacaga
     4621 aaatccaagt ccctctgccc attctttatt tgtatatgtt tcttttgagt ttaaaagtaa
     4681 caatctttta ccaagaggac ctgtttctcc cagaatttta atttttgaat tttcttttgc
     4741 aaaaacagtt ccaacaggtt cactgtaaga tgtccagaga aatggaatgc tactaggata
     4801 ttgaagaaaa taaccataag catctggagc actatttgac aggtcaaaaa catttatatt
     4861 atttatctga attaaatatt gagggtcaat aaggccaaaa agtgatattt ccttacactt
     4921 aaacagaaca atcatccttt ctttttcttt tgtgtccatc tgaaaagata tgggatattg
     4981 ctgatttcca ttaactccca tatcaggagc aaaaattatt tctccatttt ctttgtttat
     5041 ataaaaggca tttagatata ctgattggga tagagttaat cttgaaattt cagcaattcc
     5101 tttctcattt gttatttcaa ggggatgtcc acgaacaccg atatttgttt tataagttcc
     5161 atctcttgga acaactattg catcttttac agggtcattt ggaacaaaac tttttttagg
     5221 attaaatgta acaatttttg cttttagggt agccatttta tccttcattt taagttcgct
     5281 ttttgataga agttcttcat catttaaagc ataagaaaga agaaatttta atacgttaac
     5341 ctgtttgata atattctgta attttaaatt ttcaaatcca tcttttggag tgtcaactct
     5401 ccatctacca tcattaacag ttataaatga aactgcagga tttcctgaaa gaatcataaa
     5461 ttcaccatct gttctaattc tttcgggtaa aaatgtatcc caaacaactc ctttttcagg
     5521 acttattccg tttacaagaa cagattctgt atatcccaaa attttaccta ccttttctga
     5581 atattccatg aattttttac ctattggagc aaagaatctc tggtaataga aatcagtgga
     5641 gttatgccat attccaagtt tatcactatc tcctgaaagg tctagtccta taaaaagaga
     5701 tatttttatt ttatcttctt ttttaattct ttctgaaaat ggtttttcat ttctacaatg
     5761 tttttgaata aatgcatcta tccctttcat attcataaaa tgagaagaag tggcaagaaa
     5821 aataatactt ctttttggtg ggtttttcac aagatattca gcaagttcaa taagagcaga
     5881 tatcccgcag gcagatgtag caccgggaga aatggaaggg acaacacttg tactatcata
     5941 ataggaactt ataactattg tttcatcttt taatttcgca tctctccctt caagaaaacc
     6001 ataaatattc cagtcgttaa tagtttccca gtccatcctt ccataaattt ttacagttgg
     6061 ttcttttttt gctatttcaa gtattttctc attatcagaa taaaatcttg gaacatccag
     6121 agggacagaa aggaatttct tttcgccttc ggttcttgtt atttcccctc tatttataaa
     6181 aatgaaacat tttgcaccaa gcattgcaaa atcaagatag ttatctcctg aatcaaaatc
     6241 cataacaata atacttccat ttatatcttt tccttcaact tcttttaaat taccttttcc
     6301 agcatatatc aatttacctt caagtccttc tggtggtaaa gttgatgttc ttacaagatt
     6361 tggccagaga cagtttatat caattttttt tccatcatat tcaatatatg catacttttc
     6421 tacaggaaca accgatttaa aactctccct ttttacattt ttaagaccaa tttctttaaa
     6481 tttattttca ataaaatctg ctgctttttt atgtccttca ctgcctggaa atcttgattt
     6541 taaagaagaa aaaaagttta aatatgatac agtatcctga gaaaatagaa aggtagaaaa
     6601 aagaaaaaag agaaaaggga aaaaaataat cttatttctc atttttcatc tccttgattt
     6661 tttgccatgc tttccttgaa cctggattta tatttacttc ttctataaat tctttttttg
     6721 ctttttcaaa ttcccctttt ttttcatata tcagaccctt tatgtaatga gcatttggat
     6781 aatacttatt tttctctata agtttttcca tagtttccag tgattcttca tattctttta
     6841 agtaaaattc taaaagtccc aagttgaata ttaaattatc attatttttg aaatgtgtca
     6901 acccctcaag tgtatatttt ttcccctttt caaaatcgtt ttttattatg agtgaagcaa
     6961 ttattttttc ataagtcagt aggtcttttt taaactcaag tgattccatc cagcagttta
     7021 aagccatatc aatattattg tttgctaaat agtaatctcc cattcttact ttttctctcc
     7081 attttttttc tgtaaggtct gtattacaac cggaaagaaa aataattatg ggtataaaaa
     7141 tccatttcat ttttataaat tttttattct tgtaatcctt ctttttaaaa tccatacaag
     7201 aattaaaaga aatgaaaaca ttataaatcg ggtaaagata ttttctcttc ttaaaattgc
     7261 tggccataaa gaaaaaattg aaataattat taaaatgtct tcccaaatag gtgttttttc
     7321 tttaattttt cacacctttt ttaaaatttt atttatttct tttaattcaa tttcagcaac
     7381 cccaattctt ttatcagcgc atccataaaa aatataaaga attccatctt taataaaagc
     7441 acctgtagga aaaacaacat ttgggacatt accatttttt tcccagtcat aatcaggttc
     7501 aaaaagaggg aagggtaatc ttgctatttc tttttgtgga ttttcaatat caagtaaaga
     7561 tattcctgct ctgtatattt tatttttatc aactccatga tataaaagta accatccata
     7621 ttctgtttta attggtggag cacctgctcc tattttttct tcttcccaat tatatttagg
     7681 tttcattact attttgcttt caattttcct gaaatattct tcccagaaat ctttactttt
     7741 gagttcaaaa aaatcgttaa aataaacaat ttgaatatca ggttcaattc tatgaagtaa
     7801 tgcgtattta ccattaattt tttcagggaa aaggcatcca tccttatccc atataattat
     7861 attttctcct ctttctgcaa cctgtttttt ccagaagtcc ttatacctat cattttcaga
     7921 aatatttaat gcttcaagaa atgtaatgtt aggacttata atacctaatt tcttgaaatt
     7981 caataggttt tttgattctg caatagcgat tcttgcgttt ttcccatcat atgcagtata
     8041 ggtgatgaaa taagaatctt ctatttttgt tattctgggg tcttcaattc cctgtttttc
     8101 gtaaacttca gttggtgaaa taacaggata ttcacacttt tcaatttcat tttctgtttt
     8161 taattttaag tatccgagac gagaataatt ttcacatttt atagccctgt aaattatatg
     8221 aacctgattg ttttcaacaa taacacctgg attgaaaacc ccatcttttt cccattcaaa
     8281 acaaccttca tataatagat ttctttttat ttttttacct atttctttca tttttttata
     8341 cgaggtccgc tctctcttta atttatagtt ctccccccac gactaaaaac gcaggggaca
     8401 tttctttccc ctgcaacccc ttttgccgtg gggttttatt tttctcattt ccctaagttt
     8461 tcaataatta ctatactcaa actggtgccg ggagggggac tcgaaccccc acggcttatc
     8521 tcaggagggg gagattcccc cttcctgaaa tagttctccc cccacgacta aaaacgcagg
     8581 ggacatttct ttcccctgca accccttttg ccgtggggtt ttatttttct catttcccta
     8641 agttttcaat aattactata ctcaaactgg tgccgggagg gggactcgaa cccccacggc
     8701 atacgccata cgcccctcaa acgtacgtgt ctgccaattc caccatcccg gctctaaatt
     8761 tttccttcat tatagtttat attatacctt ttcctttttc aatgtaaagc aaagcacatc
     8821 tatttgcaag ttttctcacc ctctggatat atcttactct ttcacttaca ctgattgctt
     8881 ttcttgaatc aagtaaatta aaaatatgag aaagtttcag aatataatca tatgcaggat
     8941 aaattaattc cctattaaga atcttttctg cctctttttc aaatttttca aatatttcaa
     9001 ataataaatt tacatctgca ttttcaaaag aataaactga atattctctt tctctttctt
     9061 tttgaatatc tccaaatttt acatctttat tccagtataa attaaaaaca tttttttctt
     9121 tctgtaaaaa atttactatc ctgtcaagac catatgtaat ttcaactgca gggggtatta
     9181 aatcgattcc acctgcctgt tgcatatatg taaattgtgt aatttcaagt ccatctatcc
     9241 agacctccca tccaactccc cacgcaccaa gcgtaggtgc ttcccagtta tcctctataa
     9301 accttatatc atgttctttt gttgaaattc caagatattc taaactttta agataaattt
     9361 tttgtatatc aaaagggggt ggtttcatta taacctgaat ttgatgatgg agatacaatc
     9421 ttacaggatt ttctccatac cttccatcag caggtcttac agagggctca agatatacaa
     9481 cattccattt tttgtttcct attatcccaa aaaaggttgc tggatttaag gttcctgctc
     9541 ctacctcaac attataagga tgccacaaaa gacatccttt tttaatccag aaattttcta
     9601 tttttctcca gatatcttga aaaaagtttt ccataattta taattttaca aaaaaatata
     9661 gagaaagtaa aatttcaaag ggagaaaaaa tgaataatga aaaaattaaa attgtgtttg
     9721 ttggtgttgg tgcaatgggc cagtgtgctc atctgaaaaa ttatgttctt attcctgatt
     9781 gtgaggtcgt tgcaatatgt gagataaggg aaaaacttgg aaaaaaagtt gcagagagat
     9841 acggaattaa aaatttttat aaaggttttg atgaaatgat tgaaaaagaa gattttgatg
     9901 ggatagttgc atcacagcca tttacaagac attatgtaat tcttaaagaa atcctgaaag
     9961 caaaaagacc tgtttttata gaaaaaccac ttgcaagttc tgttgaaatt ggtgaaaaaa
    10021 tactggaaat tattgaaaag tcaggaacct gggtaatggt gggttatcat aagagaagtg
    10081 accctgcttc aatttatgtt aaaaaattaa ttgatgaatt taaaaaaaca ggtgagattg
    10141 gaaaattaag atatgtaaga ataacaatgc cacccggtga ctggattgca ggaggtttta
    10201 aggatttaat tatcacagat gaaaaattac cagagaatat tgaatatgac ccgaaaccga
    10261 aaaatatgga tgaatttgaa tttaaagaat atgttgattt tgtgaactat tatattcatc
    10321 nnnnnnnnnn atcagataaa ctttatgaga tttattttgg gagaggacta caaagttaaa
    10381 tttgcggata gttcaaaggt tttaatggta gtggagagta attcaggtat ttcaggagtt
    10441 atagaaatgg caccctataa tacatctatt gaatgggaag aaaaagtttt tatatgtttt
    10501 gagaaaggat atattgaact ttcgcttcct gctcctcttg cttcaaatag acccggtaaa
    10561 gtcaaaattt ataaagatat tccttcacct ttaacaataa ttcctcactt cccatgggta
    10621 tctgcaatgt atcagcaggc agtaaatttt gttaaagcaa ttaaaggaga aataaaacca
    10681 ccaaacgatg caaaagaagc attcaaagac cttaaagttg cagaagaata tttaaccctt
    10741 tttaaaaaat aagtaaatca gattaagtcc accatgcttt tggctttggc tctttaatta
    10801 atacattttt caaaaattca attgctttct taagtccttc ttcaccactc attaaactat
    10861 cctcatgttc aatacttaat acaccatcat atcccaccat tctcaatgtt gaaataaaat
    10921 cattccagaa ttcatatcca tgtccatatc caactgttct gaatatccag gacctatttt
    10981 cttcatcaag atatgttttt gtatcaagaa ctccatttat acttgatgtg taagggttta
    11041 tttttgtatc ttttgcatga acatgatata ttgcaccttt aagtttcctc acagccgcta
    11101 tcgggtctat cccctgccag aaaagatgac tcgggtcaaa atttgctcct atttcttcac
    11161 ctgctgcttc tcttaatttt aaaagtgttt ccggattata tacgacaaaa ccgggatgca
    11221 tttcaagaca tatttctttt acattatgtt ttcttgcaaa tgctgtttct tccttccagt
    11281 aaggaatcac tactttttcc cactgccatt ttaaaatttc tgaaaaatct gtgggccatg
    11341 gacatgttac ccagtttgga tattttgcat tttcattatc tccgggacaa ccagaaaatg
    11401 ttataattct tcctactcca agtttttctg caagtaaaat tgtcttcctc tgaacctgcc
    11461 tgtgtgattc tgctatgctt ttgtctggat gaagaggatt tccatgacag gataaagcag
    11521 atatttcaag acctcttttt tctattgctt ttttaaactc ttttaatttc ccttcatttt
    11581 caagtaattc accaacctta cagaaagcat ctcctggata attacctgtt cctatttcaa
    11641 ctgcttcaag tcccaattcc ttcaccttat caagtgcttc atcaaaactt ttacccccaa
    11701 aaaccactat aaaaacaccc agtttcattt ttccctcctt tttataaata ttataaacca
    11761 aataaaaaat tttcaaaatt acattctttc cggtagagaa acatttaaaa gatttagaga
    11821 taattcaaaa attataagaa gcgactttac aagaaaaagt cgtgcatatg ttaaatcctt
    11881 ctctccccca actattctgt atttctggta atatgaatgg aataactttg aaacatcaag
    11941 taaatattca gcaagtaaat gaactccata tgttttaaca acacttctta taacatcatt
    12001 aaatttccat atctttttca ttatttcaat ttcttcttct ttatttaaca aatcaatctc
    12061 cggtataaag ttatcaattt tatatccttt ttcttttccg aattcaatta gatgtttcag
    12121 acgaacatat gcatattgaa ggtaataaat aggattttca tttgactttt ttttagcaac
    12181 ttctaagtca aaatcaagat gactgtctat ttttctgaaa agaaaaaaga atttggaagc
    12241 atccggacct atttcattaa caagttcctt taaagttata aactgtcctt ttcttgttga
    12301 cattgataat ttttcttttc ctctatatag agttgtaagt tgaactatca gaacagaaag
    12361 attgtcggga ttaaaaccta aagaagcaac ggatgctttc aatcttggaa tatatccgtg
    12421 atggtccggt ccaactatgt tgataagaaa ttcaaaaccc ctatcaattt taaatttgtg
    12481 ataagcaata tctgttgcaa ggtatgtata acttccatca ctcttaacaa caaccctatc
    12541 cttatcatct ccaaaataag tacttttaaa ccataaactt ccatcttttt cataaatcaa
    12601 atttttttct tttaagatat ttaaaacatt ttcaacttct ttatttttaa tgaactgact
    12661 ttctctaacc caattatcaa aagaaacgcc aaaattttta aggtcatttt taatatcttt
    12721 aagtatttca ttttctgcaa attcggtaaa aaattttttg tcatctttgg tttcttttat
    12781 cttccttgca atatcaatta gatactcacc aagatatcct tcttcaggga ctggataatc
    12841 aactccttta agttgataat atcttgcctt taaagattct ccaagtaatt ccatctgtct
    12901 tcctgcatca tttatatagt attcctttgt cacatcaaaa ttacagaatt ttaatattct
    12961 tgaaagtgat tcaccatatg ctgcttgtct tccatgagca attgttaaag gacctgtggg
    13021 atttgcactt acaaattcaa taaggacttt tttcccgtta cctatatttt cctttaagta
    13081 ttctttttga ttttgacaga tttttaaaat atgctgtctg taaatatcct catcaataaa
    13141 aaaattaata aagccattct ttacttctat ttttttaatt attcccttta aatcaggata
    13201 gattttctct tttataactt cagcaatttt ttgaggtgaa ttttctttat cagcaagtct
    13261 gaaggagata tttgtataaa aatcgccaaa ctcttccttt tcaacaggta aaacatcaaa
    13321 atttacatcc tttttaaagt caattataga tttttttaat gtattttcaa ttgtactttt
    13381 aacatcaatc ctcattttct ttctttatct cttttaaaag ttgctcaatc cttatttctt
    13441 ttctttcatc aagttcaaat tcaatacttg gtgtaaattt aagggatatt ctctgtccaa
    13501 gtttatgctg tataaatttt tgagcactta aaataccacg aaaagcattt tctttttcct
    13561 tttcatcacc catgaaactt ataccaactc taacattttt aaggtcgcct gtaatatgaa
    13621 catatgtaat tgtaaaaaaa cagattctcg ggtctgaaac atctcttctt ataatatttt
    13681 ctatttcttc tcttaacata ctttcaactt ttaactgtct tcttgtttta atttttttct
    13741 cctttttgtt atagaatacc agaaaataaa ctttaattca aacctttatc ccacctttaa
    13801 ggtgggactg gagaattgtt gatagacaag gttttccaga ttaggttttt caatcctgtt
    13861 gtcaatgtaa aaaataggtg gtaaattata tttaagatga aatggaagaa cttagaaaac
    13921 tgggtaaaag gtattttaat attcggtttt actttttatt tgtttttttc ctttatatct
    13981 tttgaccctt ttgaccctac atttaaattc tgtcagatag gttttccaat aaatgagaat
    14041 ataaaaaatt ttggtggaaa attcgggtca tatctaacag gacttctatt ctttttattt
    14101 ggtaaaagtt catacctgat tgtaatcttt atgcttttct taggatacaa tatcatatgg
    14161 ggagaaaaag aaagattatg gaaaagaata acaagtatca caggtcttct tttaacatta
    14221 tctgtatttt taacagtaat cagaggttct ctgggaaatt ttgatggagg acttttagga
    14281 actgttttaa cgcctgtttt aaaagagtat tttggagaaa atggtatatt ttttattctt
    14341 ggactttcag ggattttatt tattggtata agttatagaa tgtatactga accgtttaaa
    14401 gtagttttca atgattattt ttttaatgag gataaaattc ctgttcctga aaaggtaagt
    14461 ttaccacaga agcaaaagag aaaaatattt gaaacaaaag aaaagataga aattccacag
    14521 ccaaaggaag aaaagttaga agagaaaaaa acagagacag aagaagaaat aaaggaggaa
    14581 aaggaaaaaa agaaaataaa gaaaacatct atttcacgac aaaaaggtgt agtttataat
    14641 ttacctcctg ttgaccttct tgaaatagga aatcccacac aaaaagaaac aaaagaggac
    14701 ttggaaatat atgctcaggt gatagaagaa acactttctg aatttgatat agaaggagaa
    14761 gttgttcaaa taaatcaggg acccagagtt acaatgtttg aagttcaact tgcaccaggt
    14821 attcaggttc aaaagatttt taaaattcag gacaatattg ctatgaatct gaaaacaaca
    14881 actataagag ttgttgcacc tttaccaaat aaatcaacag tgggaattga agttccaaac
    14941 agagaaattt ctatcgtata tttaagagag ataattgaaa gtaaagaatt tcaacgttct
    15001 ccttccaaat taacacttgc tataggaaag gatataatgg gtagaccaat tgttacagat
    15061 ttaaaattga tgccacatct tttaattgca ggagcaacag gttcaggcaa aactgtttgt
    15121 atcaattctt taataacttc aattttatat aaagcaagtc ctgatgaagt taaatttata
    15181 atgatagacc ctaaaatggt tgaacttact ctttacaatg accttcctta tctgctttgt
    15241 cctgttattg ttgatattaa aaaggcagtt aatgctttaa aatggttaat cggtgaaatg
    15301 acaagaaggt ataaactttt ttcagaacat aaagtcagga atattgatgt ttataataat
    15361 atttttaaag ataatccatt gccttatatt gtagttgtca ttgatgaact tgctgatttg
    15421 atggcggttg caaaaaatga gatagaacac agtattatac gacttgcaca actttcaaga
    15481 gcagcaggaa tacatttgat tttagcaaca caaagaccat cagtaaatgt tataacaggt
    15541 gtgattaaag ccaatttgcc atgtagaatt tcttttcagg taccatctaa atttgattca
    15601 agaacaatcc ttgatagaat tggtgctgaa aagttacttg gtaggggaga tatgctgttt
    15661 ttaccacctt taagttcaag tttgataaga attcaaggaa gtttcatttc agatgaagaa
    15721 atagaaaaag tatgtgaatt cataaaaaaa cagagaatcc ctgaatataa tatggaaatt
    15781 cttgaaagga agacccagga agaagaaact cctgaaatat tacaaacaga atatgatgat
    15841 gaagaggttc tttatgaaca ggcaaaaagg attgtgatta cgactaaaat tgcttcaatt
    15901 tcaatgcttc agagaagatt gagaattggt tttaataagg cagcaaggtt gattgaaagg
    15961 atggaagaag aaggaattgt tggaccgtat caggaaggga agccaaggaa agtaataata
    16021 aatatggaag agaatcagca aaattcagaa gacaatggat ttgaagagtt taagtgagtt
    16081 aataaaaaaa gaaagagaga aaaaagagat tgattacgat aggatttaca gagataccaa
    16141 aatagttagg aagttcatag aatatattga agatggagaa tggaataaat ttccgggtaa
    16201 tttttacaga aaagcagttt taaaaaaata tcttgattat ctggggattg aaggaataaa
    16261 tctggacgaa ctttttgaag aaaaaaaaga aacttgtgag gaagaaatta ttgaaccgaa
    16321 aagagaaagg aaagatgaaa gaaataaaga aaaaaatgaa aaaaaatttt attctgatac
    16381 gaagttttat attttaattt ttctgtttct tcttttcatt ttacttcttc ttatagggaa
    16441 tttacttttt gaaaatctta taaaatgaaa atccatctta tttctctcgg atgtcctaaa
    16501 aatcttgttg attcagaaca gttacttgca attttaggag aagaaggttt tataataact
    16561 gattcaatag atgaagcaga ccttgtaata ataaatacct gcggttttat aagagaagca
    16621 gtaaaagaat cagaagaaaa cataaaagaa attatgagaa aaaataaaga actgattatc
    16681 tggggatgtc ttgttcagag agagaaagaa aaattactta aatttggaaa attgaaagga
    16741 attgggggag ttggtaatcc tgaagaagtt ttaaaaatta ttaaggagaa aaaagaaggg
    16801 attattagaa taaaggaaag tttttttacc tgcagaaaag aatttccccg tcttataaca
    16861 acttttcctt atgcatacat aaaaatttct gatggctgta ataatttttg cagttattgt
    16921 ttaattccta aattgagagg tcctttaaga agcagaaaaa tcataaatat tttaaaagag
    16981 gcagaaaata ttgaaaaact cggtttcaaa gaaattatac taattgctca ggatacaaca
    17041 aattatggtg aagatttaaa agatggttca aatttgatta aattacttga agaacttgaa
    17101 aaattcaatt ttgaatggat aagagttatg tatatgcatc cgggacatat aaaagatgaa
    17161 ttgattgaaa aaataggagg tagtaaaaaa atatgtaagt attttgatat accgatgcaa
    17221 catgtgtctc aaaaaattct tgaaaagatg aatagaccga ttattgacta taaaaaatta
    17281 attgataaaa taagaagcaa agtaccttct gctgcaataa ggacaaattt tattataggt
    17341 tttcctggcg aaacagaaga agatttcaga aaacttctgg aatttattga agaagttaaa
    17401 attgatagag ttggcttttt taagtacagt agagaaaaag gaacagttgc ttataatttt
    17461 aaagaacaaa tagaagaaaa aataaaagag gaaagaatga aaatccttat tgaaaaacag
    17521 aaagatgtat cagaaaaaat tttaagaaaa tatactggaa aggttttaaa ggttcttata
    17581 gaaaaaaaag ataatgattt ttatattggc aggactgaat atgatgctcc tgaaatagat
    17641 ggtcttgtgt atgtaaaaga gaaaaatctt aaaacaggtg agttttataa tgttaaaata
    17701 aaaaaaacag attcatatga cctttatgga gaaaagatag gatgattata gaaaaaataa
    17761 ttgttggtga tgtttttaca aattgctata ttgttgggtg tgaggaagaa aaaatatgtg
    17821 gaattataga ccctggagat agcggagaaa aaataaaaga aaggatagaa gaactcggtt
    17881 taaaacctgt aatgataata aatactcacg gacattttga tcatataggt gcaaatcatt
    17941 tattcaatct tcctgtttac atccataaag atgattttga atttttaaaa gaccctgaaa
    18001 aaaatctatc ttcatttttt tccgtccctt atgtaagtaa aaatgacaat attattacag
    18061 ttgaagaaaa agatgtgata aaaataggga aattatattt tgaggttata cacacaccag
    18121 gtcatacacc aggaagtata tgtcttaaaa tagaaaatat tttatttaca ggagatacaa
    18181 tttttgctga tggaattgga agaactgatt ttcctgaagg agatgaagaa agattgataa
    18241 aatcaataaa agaaaaaatt cttccattac cggatgattt tattatttat ccgggtcatg
    18301 gtaaatctgc aaaacttata gagtttaaaa atctattttt tttatgaaaa cagaaccgat
    18361 tattggaata agcagtttac attttatatg gcatagttta gaagaagcat ttgaaagatg
    18421 tgcagttgaa ttcggtttta aatttataga attttcttac tcagatttat taaaagaaaa
    18481 agaccttttg cttatgaaag aatttaagaa aaaatataat atagaaactg gattacatat
    18541 ctgggataat atacctgaaa ttggtaaaga agaagcaatt ttgaagatga aagattattt
    18601 aaatatctgt tcaattgctg aaatcagttt tttaaccgct caccttggaa aatattctga
    18661 aagagaaatt ggacttaata ttgtttttga ggttctaaaa aatgtaatct ctttatatga
    18721 gaaaaaaaat attaaaatct gtattgaaaa tcattatatt gatgaacttg gcaacagccc
    18781 tcaagaattt ctttactttt ttgaaaaaat aaattcccct tctttaaaat tctgttttga
    18841 ctacggacat gcaaacttaa atggagatat ttttgaattt atagaaaaac tttataaatt
    18901 ttttggtatt gttcatgtat ccgataataa tggagataaa gatgaacatt tacattatgg
    18961 agatggaaat atagaatgga aggaagtttt atctttaact ttgaaaaaag gtttttttgg
    19021 tccatatata attgaatttg gtggaaaaaa tgaaaagata gagaaattta aaagttttga
    19081 aaatgattta aaagagatag caaagaattt atagttttat tttttccaga aattcaatat
    19141 gttttgaaaa atctccaact tcaattaaat tatgagaatc tgaaccaaaa acaattttca
    19201 cattttcttc catacacatt ctgaaaaatc tatattccgg attattatga tggtaattta
    19261 tttctgcttt tacctttcct tctttcaaca tttttacaac atccctgtat aaatattctg
    19321 gcttttccag attttttctg taaaaaaatc tgaaaggatg agcaagtata tcaattttat
    19381 tttcaatgag tttttcaaca gaccataaga actttttttc tgtttcactt ttacttgtac
    19441 accactcatc aggtaaataa tgaactgctc caacaagtat ttcaaaaaat tttctgtcct
    19501 cttcaagtaa tgatattttt ccatttttat caatttcaac ttcaagtcca attccagcca
    19561 tattagaatt cagagattta aatttttcta cataatcttt aatcctatcc tttttttcat
    19621 ctctctgttt ttttaaaata tcagggttat aaaaaaattt gaaattccag taatcttccg
    19681 gctgtagata aagttgtcct gcatgttctg taaaataaac tttttccaca ccaaacaact
    19741 tacatctttc aatcactttt tccatactaa catcttctgc gcagtatcca aattcagtat
    19801 gacagtgaaa gtcagtagcg gttattttat tcttcaattg tttttcttca atttttaatt
    19861 cacttgaaat ttcaaccaaa aaatacttaa atggttcttc acatacagca ggtaaggtaa
    19921 aatagtaaac tccttcatta ttaagaatat ccagtcccct gtggtaatgt ccacttattg
    19981 aaagaaaaac attattttct ttataaagtt tgtgaatttt ttcacagtca agtaaattaa
    20041 aagggtaatc actttctatt tttggaaaga taatattgtg ctgaactgct ataatttttt
    20101 tatctggatt ttctcttaca atttttttaa aattctctat ttcttcctga cttctaattg
    20161 caatttctcc ttcataatat ctgtcgtaaa aaacatagaa taaaaagtta tcataaacaa
    20221 aatatttata atcaccaaaa atttcaaaaa attcttcaat ttttttatca tgatttcctg
    20281 gtgcaataat aacttttttg gcattaaaat tatcaattat cttttttatc tctaaatatt
    20341 ccttttctga atcaggattt tcaccatcat caagtaggtc tcctgcaatt ataacaacat
    20401 caatatcttc ttttaaattc ttctgtattc tttttaaaaa ctcaatcccg aattttacat
    20461 ttctttcagg gatttttgaa ataccatcac ttttataatg taaatcactg agaagtagca
    20521 gtttcatttt tcagtgtttg aaatgtcttg tccctgtaaa aatcattgaa atgccgaatt
    20581 tattacattt ttcaatgaca tttttatctt ctattgaacc accgggttga attatagaag
    20641 ttactcctgc ctgatatgcg atttcaatac tatcaggttt ggggaagaat gcatcagaag
    20701 ccaaaacaat aggtttcggg atttttttaa aattttcttt cattttcata actgctattt
    20761 ttgttgaatc aactcttgac atctgccctg ctcctatacc gagtgtctgg tcttttgttg
    20821 ctataacaat cgcatttgat tttacaaact tacagacctt ccacgcaaat ttaagtcctt
    20881 caatttcttc gtcagtcggt tttttttctg atacaatttc aaacttttca taatcaattt
    20941 catcgggtgt ttgaactaaa aatccatctg caagagtcct gaaatcatat tcatatttta
    21001 ttctttccgg acattttata acccttaaat tcggcttttt cttaaatatt tccagtgctt
    21061 cctcttcata gtcaggagca ataattatct cataaaatct ctctataaaa atttcagcaa
    21121 gtttcttatc aactttttta ttaaatcctg caattcctcc aaaagcactc gtttcatctg
    21181 tttgaagagc ttttttataa gcgtctatta aatcacttcc agtggcaact ccacagggat
    21241 ttgtatgttt tattatgaca caggcacact tttcaaattg acagacaaga ttatatgcac
    21301 tttccatatc aagaagatta ttaaatgaaa gttcttttcc ctgaaaaatt tctcttttaa
    21361 attctctgcc agtaaatcta taccatgcac ctttttgatg tgaattttct ccatatctta
    21421 aattttgaac ttttagaagt ctgatatcca tttctgaggg taaaatttct ttttctattt
    21481 tatctgtaaa gtatgatgag ataataaaat catatcttga agttaatctg aaaacctctc
    21541 ttgcacattt gaaagaaaat tcctcactta ctgttccatt gtttttttcc atctctttaa
    21601 taaattcttc atacatttca gttttaacaa gaacacatac atatttataa ttttttgctc
    21661 ctgctcttat cattgttggt ccacctatat caatattttc aattatttct tcatcacttt
    21721 tccccatctt aagtgttttt tcaaaaggat aaagattgca tatgatgagt tgaattggct
    21781 ggattttatt ttcttccatc tgtttcaaat ggtcttcttt gtccttaatt gaaagaatac
    21841 caccataaat ttttggatgc agtgttttta ccctgccgtc aagtatttca ggaaagccgg
    21901 taaactgact cacttcaata acatctattc cgttttcttt aagtgttttt gctgttccac
    21961 ctgttgatat tatttcaaca ttatatttct ttaaaaattt tgcaagttca ataatacccg
    22021 ttttatcaga tacacttata attgctcttt ctatttttat cataagttac ctccattttt
    22081 aatataatcc ttcctgtttt tctaatattt tttggacttc taaaggattt accttatcaa
    22141 ctgaaatgtt cttatcaaga gcaatatagg cacctattcc agcagaatgt ccggttgcaa
    22201 tacaggttgc cattactctt attgaactca ttgctttatg gtctgcagaa atacatcttc
    22261 ctgtaacaag taagtttttg gaattttttg gtatgagaca ttcatacggt atactgaacc
    22321 aatcattttt aggaggatat aatttatttt ttgccggaag gtaatcctta aatttatcaa
    22381 gagcaataca gtattcctcg gtttggcatt ctttaacgca taaatgaact ttttttgttc
    22441 tgccaagagg gcagtgaatg tctatccagt aagtacctct tgcaatactt gaatcaaact
    22501 ttctcccttt taaaatatcg tcttctgtta gagtatagag tccttctatc tgtcttgttt
    22561 cccttattcc tatatttggt ggtaaagcag atatatatga gctttcaaac ccagggacat
    22621 tttctttcaa aaatttccag tatttataaa tctggtctct tataaaaatc tcaccttttg
    22681 tcaggtcaaa tacatctgtt ccatcacctt gaaatctcat aacattaaat gacctttcgc
    22741 cttttcttgt tgtactcccc ttgcctccaa gtcctgtatt gtaaatgatc aattctcctt
    22801 tttcttttaa ttcgtttaat aaatttcctg ctttctttgc aacttcctct gttatttttt
    22861 cttcgtcaat tccggcaatt ttaaacatac atcccattga ctgcattttc ccatcaaaaa
    22921 atctaccttt tgtataattg aatccagaat aaaaggcaat atctgcgtct ccagtagcgt
    22981 caataaaaac cttccctttt attttcattt ttccgctttt attcacaact tcaatttctt
    23041 ttattaaact atcttcaatt tttactgatg atacaaaaga atgatataaa atttcaactt
    23101 tctcttccag caacaatttt tcagaagaga ttttaagaat ttcagaatca aaagaaatac
    23161 catattttct aaaagcagat tcccactttt cgcatccatc catttgatat gttttttcaa
    23221 ttaaaaattt taaaaatcca cttacagcag gttctttttc attcaaataa tggccaagta
    23281 ttgaaccaac aaatccagca gtagccattc cacctaaaaa tccatatctt tcaataagta
    23341 aaacttttgc tccctgtttt gcacatcccc aggcagatgc catacctgca ggtccgccac
    23401 caataacaat tatatccttt tccatttttt ctccttttta aaaaagaaat ttttttaaaa
    23461 tgatataatt tatattgaaa atggtcaata tatttaagag agcagaggaa tttgtcagta
    23521 aaaataaatt gattgagcaa ggagacagaa ttttacttgc tgtttcaggt ggtcctgatt
    23581 cggtttttct tttttatttc ttcattcatc ttttaaaaaa gaagaaaata gatataaaag
    23641 ttgcttatat ccaccatcat ttaagaaaag aggcggatag agaagttatt tttgtgaaaa
    23701 atctcacaaa taaatatgga gttgaatttt ttagagaaga tattgaaatt aaagaaaaga
    23761 aaaatattga aaaaaactta agagaaaaaa gatatgaaaa actttatgaa attgcagaaa
    23821 aaacaggatg taataaaatt gcaacaggac atactcttga tgaccatata gaaacgtttt
    23881 ttatgaatct ttttagaggt tccggtacat ccggtctttg cggtatatgg caaaaaagca
    23941 atatttcccc tggttctgaa atttttatta taagacccct tctctgtgtt gaaaaaagac
    24001 agattgttaa atatcttgaa gaaaataaaa ttgaatatct tattgatact tctaatttat
    24061 cttcaaattt tttcagaaat aaaattagaa atgaggttat accattttta ctcaaatata
    24121 gaccctcttt aaaaaaagta attctaagaa caactgaaat tttgaaggag gatgagattt
    24181 ttttaagaaa atatacagat gctattttta aagatattac aatgaaagaa aaaaataaaa
    24241 ttattattga tgtggaaaga tttaaaaaac ttgataattc cataaagagg aggattgcat
    24301 caattatata ccgtaaaatt aaaaataccc cctatataaa cttttccatt attgaaagat
    24361 tgataaaaaa tattgaaatg ggtgaaaatg tttataatag tgaaataatt gaaaaaattt
    24421 taaaaggaga agaaaaagag gataaaagtt ttatttacag aattaaagtc ccaggagaaa
    24481 tagaaatact tgataagttt atcattagaa gcagttttgt tccgttttca gataaaattt
    24541 ttaaaaatga aaataaattt acaggttatt ttgacttttc aaaaataaag ggagatataa
    24601 ttgtaagaaa tagaaaggga ggagacagat ttatgccatt aggacttaat aaagaaaaaa
    24661 aattatcaag atttatgata gataaaaaaa ttccagaaaa agagaaagat gaaattttaa
    24721 tttttgaaaa taatggtaaa attatatggg tttgtggtta tgaaatatct gaacagttta
    24781 agataaataa aaatacagaa aaaattctaa aaattgaagt gagaagtaag aattaaaaat
    24841 ggaaaattat attatggaaa gaagtacaga aaaaattttc cctgataatt atttaccaaa
    24901 agaagtaagg aataaaaaac taaaagatat agtagttctg ggttctcaat ataaaaaaga
    24961 agaaagtatt gaaattattt attatttaaa atgtgaagga gatattattg aggttgctaa
    25021 aaaacttgcc atggatgaaa caactggaaa atggatagga gatggaaaac caacttcttt
    25081 attcaaaaaa tgtgttgctg atgttgataa agtttatatc tatgataagg gagaaggaat
    25141 tgtttttata agaacgccaa ttattaattt atcagaagaa aaagaccctc tttatcagat
    25201 tttaatgctt gcatgtggag gtcctgttct tgaatttgtc tattattcag atgttgctct
    25261 tatagatatt aatttacctg aaaaaatact taaaaaattt ccgggtccat cattcgggat
    25321 agaaggaata agaaaactta cagaaacacc ttatcctgag ccaatagttg gaacaatagt
    25381 aaaaccatgt gcgggaatga ctgagaaaga agttgctgaa aagtgttacc ttgctgcaaa
    25441 aggaggagtt aaatttataa aagatgatga aaaaatgctt ggaccatctt attgtgatat
    25501 aaagaaaaaa ataaaacttg ttagtaatgc attgaaaaaa gtttatgaag agataggtaa
    25561 taaatgtatt tatgctcctc atcttgttga aagagcagat agaattaaag atacagcaaa
    25621 aagatatatt gactatggag caacagggct aatgctgaat gttgttcttg gacataattt
    25681 tgaagtttta aaaattttaa gggaagataa agatataaat gttcctctat atgctcacag
    25741 tggtggaaga agtgctttat caacaggaaa cagaagaatt gatgatgctg ttattgtaaa
    25801 gtttattcgt ctgtgtggag gtgatttttt tcagcatgga gtttttggag taaatgatac
    25861 ccatattgct tctcttgatg aagatttatt gaatcatttg atttttgtta tgagatataa
    25921 cttaaaagga ataaatgata caataccagt tgctgcaggt ggtttaagag ttgataactt
    25981 aaaattgaat tttgagagac attatgatga gaagtttggt tttggagttg ctttacttgc
    26041 aggttcgtat ttactcggtc atcaaaaggg tccatatttc ggagcaaaag agtttttaag
    26101 ttctgtgaaa aatctgataa aatttatacg aagtaaatga gaaggagaaa taaaaacgga
    26161 attacactga tggaggttct tgtttcaatg tgtattatat tttttattgc tgcctcttct
    26221 ttacctgttt tacatagagc aagaaataaa gcagtcatag cgaggacaaa agcaattata
    26281 aatacaattg aagcagcact ttctatgtat gagacagatt ttggtgatta tccagaaggc
    26341 gaaggtaatg gtagtaaaat tattgtggaa ttgttacagg gtcctgttga tagtgacaga
    26401 tggcgtggac cttacataag attaaaaaaa gatgaaattg atagagaagg taatattctt
    26461 gatgcgtgga aaatgccgat tatttataag tatcctcaga cagattatga taatatacct
    26521 tttcttttgc tatctgcagg acctgatagg aaatttggaa caaaggatga tatagggaac
    26581 tggtaaaaat aaaaggggga aaaaatggaa gaattagtaa aatggcagga gaaaaaaaga
    26641 aaaaaacttt ataaattatt cggggaattg cctgaaggag attatcctgt aagagtaatt
    26701 aaaagaattg aagaggaaag agaaaaatat attcttgaaa aacttgttct tgacctgaat
    26761 ggatatgaat tggttcctgc ttattttgtt aaacccaaaa atttgaaaaa tagagttcca
    26821 gcgattgttt tcaaccattc tcatggaggt aaatattatc ttggaaaaga tgaatttatt
    26881 gaagggaatt cctatatggc agttcctcct tatgcagaag ttataacatc attaggattt
    26941 gtaggtatat gtattgacca tctaaatttt ggagaaagaa gtggaaggga tgaacttgac
    27001 ctttttaaag aacttatatg gaagggaaga gttttatggg gacttatggt ttttgacagt
    27061 ttaaaagttg ttgattattt gatgacaaga gaagaagtag ataaagaaag gattgcaaca
    27121 attggaatga gtatgggttc aacaatggca tggtatctat cagcccttga taaaagaatt
    27181 aaggtatgta ttgatatctg ctgtttaaca gattttgaag aactcataaa aacgagaggt
    27241 atttcaggtc atggaattta ttattttgtt ccatcattac ttaaattttt ttcaacatcg
    27301 gatataaact ctttaattgc tccaagacct catctttcag ttgcaggtgc atttgataga
    27361 ttaacacctc ttgatggact taaaaaaatt gatagagaac tgaaaaatat ttataaaaaa
    27421 tttgggaaag aagaaaactg gcaacttaag atttataatg taggacacag agagacattt
    27481 cagatgagaa gtgatataat tgaatttctt ttaaaacata tttaaatgga tagatttaat
    27541 ttaggaattg aggttataaa aaaagcggga gaatatttaa aagaaaaaat taatgaatgt
    27601 aaaaaactga aagaaaatag atatgatgtg aaacttttgc aggacattga aactgaaaag
    27661 attattgtag aaaatataaa aaagatattt ccagaagata gttttttatg tgaagaaaga
    27721 ggaattgata ggaaaaataa agataattta tggataattg accctcttga tggaagttta
    27781 aatttttcaa gaggtattcc acatttctgt atatccattg cttttaatgg gaaaaaggaa
    27841 aagttcggta ttgtttatga ttttgtaaga gaagaaattt ttaccggtat tgaaggtaaa
    27901 ggtgcttatt taaatggaaa aataataaat gtaagtaaaa cggataagat tgaagagagt
    27961 attttaagca ttggttttat gtctggagaa gaagaaattg agtatggatt gagaatttta
    28021 aaagagatgg gaagaaaagt taaaaaaatc agaatgtttg gttctgcttc tcttgactta
    28081 tgctatcttg cttcgggtag aatagattta ttaattcatt taaatttaaa aaaatgggat
    28141 tatgaaggtg gaagaattat attgaaagaa gcaggcggta tatttaaaaa agaaaaaatc
    28201 aataatatag aagtttttaa aggaacaaat aggagattga agatataatg gcaaaaaaaa
    28261 gtatacgact ggaagatgtg gtattaaaac ctgttacagg tttaatagaa agattcaata
    28321 tatttttgaa aaatatggaa acaaaagaaa aggtatcaga agtggatggt tttgtttcgg
    28381 gtttgctgga tgaattaaaa gatatagaaa aaggtattct tggatttttt gctgaaatat
    28441 tatttatgta ccatctttca gaaagaatgg taagtattga gaatgaaata tctcttgttg
    28501 aaattcttgg agagagagta aaggaatttt taaaacctga ctttataaaa atctatttaa
    28561 aaacatctga aggacggatt tcttctgctt atagttatcc agttgatttt gaagatgaaa
    28621 ctataaattc aattcttgaa gaaagtttac aaaaaggaga aagtttttta tatgaaaaga
    28681 aagttgatgg caaatattat tcaattttat caataccctt aagaacaaca aaggaaaaat
    28741 atggtttttt tataacagga aggaaaagaa aaaagggttt tactccagag gaaatttctc
    28801 ttctgattgc aggttcaact gttgtcagtt ttacaatttc aaatttaaaa ttgatgcaga
    28861 aaatgattgt gaatgaacga cttgttatga ttggacattt aatttcagga ctttcccatg
    28921 atttaagaaa tatacttaca aaatttgaaa atgggattta cttcatagaa tcaggaatag
    28981 aaatgaaaga tatggaagac attaaaatag ggaaagatat tttgaaaaaa aattatataa
    29041 gattaaaaga gtttgtactt tctatggttg attattcaag ggatagagaa gtatcttttg
    29101 aaaatgttga tttacattct ttaattgaag atgttatttc atattttgaa acaactctta
    29161 aagagaaaaa tattaaaatt ttgaaagaat ttgatgagaa tttaaaactt gtaaaaattg
    29221 acaaacacag aatagaaaga atgatttcaa atttaattca gaactcaatt gatgctgtta
    29281 aagaaaatga aggaataata aagataaaaa ctaaattgat ggaaaattct tttcagatta
    29341 tagttgaaga taatggctgt ggaatcccag aaaaaaatct tgataagatt tttgatattt
    29401 tctttacaac caaaggttct cgcggaactg gttttggact tgcaattgtg gaaaaagttg
    29461 ttaaagagca taatggtaaa atagaggtga gttcaaaagt tggagaagga acaattttca
    29521 aaataacttt gccaagatta taggagattt ttttgaaaga tatttttatt ctttcagtag
    29581 ttcagggtat atgtgagtgg tttcctgtga gcagttcggg tcatcttttt ctattacaca
    29641 gaattcttgg tataggccag gatataaatc tggacatttt tttacacttt tcctctcttc
    29701 ttgcacttat aatctttttt aaaaaagaaa ttttaaatgt tttaaaaggt tttttatctt
    29761 ttgatgttaa aaatgaaaat tttaaaatgt cctggtatat tatttctgcc tctattatta
    29821 cgggaataat tggattttta attaaggata aggaatttct tgaaaataaa aatgttgttt
    29881 ctgctggttt tttaatcact acaattttaa tttttttatc aggtaaaaag ggagaaaaaa
    29941 aagttagttt tacaagttct cttattatag gttttttaca gggaattgct cttattcctg
    30001 gtatttcaag gagtggtaca acaatttctg ttgctaaaat tttagggatt aaaaatgaag
    30061 atgcatttaa tttttctttt ttgcttgctg tccctgcaat aattggagta actttattga
    30121 aaattgacca gataaaaaat ataagagttg attatattat aattggattt ttaacaactt
    30181 tttcagtcag tattataact ctttattttt taaaaaagat tatgctcaaa aatagatttc
    30241 aatatttctg tatttacacc tttatggttt tcttgttttc tttattaatt aactgatagg
    30301 atattttcca aaattaaaac ccgcttcaag aaattcaata taactttgtt caagttcagg
    30361 tgtgattgga gcagattcag gaatatcgga tggaattaaa ataaatcttc ctctccttgc
    30421 tccctgaatt aaacaatcct ttaccatatt tcttatttct tctcttgttc tgaagtcaaa
    30481 atcatcagtc ataggtccac ccataattgt gattgaatct ccaacaattt ttcttacttt
    30541 aagtattcta ttaggaattt caaaactatt acatatgggt tcaatgatat caattggcat
    30601 tttcaaaata ataggtaaaa gttcttctat gccatcatgc cagtgataac aaacatagtt
    30661 gaaatttcct cttttcaaaa tctcaattaa tgtcttatca aatctataaa catattctgt
    30721 aaaaactttt ttaatgtcat gaaaatattc ggatggtaaa tttggaggcg ttgcatattc
    30781 tgcacctctt attcttataa tagcatttga gatagaaaat gaaataaatt tataaaggtc
    30841 aagtaatctt tcatatattt tttcaagtaa ctgcataatg ggttttggat aatcaagagc
    30901 aaatttaccg aaatctcctg gagcaaatag aaagccaaca actccaagag ggtcaggaag
    30961 agctactacc atcactcctt tatctccaag ttttttttca agttcataaa aatcagtaaa
    31021 atctggttga tagggaatat atggtaaaga taaaaatcta tcaatatctt tttctctctt
    31081 aatccatggt tcaacaaccc actgatgtac acttaaattt gaagttcttg ctattcttgt
    31141 taaaggtcca tattctgtct caaccgttaa tttcactgtg gttgatattg gtgtatcatg
    31201 cgtttcctct actttaaggt caatagaacc aggagcagaa aagaaaaaac ctgttttagg
    31261 tctgtaaaaa acaaaagtat cctgaatttg ctgtgccttt ttgagtaaat tttcaaaact
    31321 tttatgcttt gtccagaaac tgtcattatc aaaaggattt atcttataca atgaaacagg
    31381 aactctatca ggaaatccac ctcttaaaac agcataaatt ctttctctac ttgtcattat
    31441 tttcttccca gtattttatc tattgcaagg gaagtagtat aaatttttgt ttccggataa
    31501 tatttataag gtccaagttc tgcagttaaa aaacttgtat aaccaatttc ttttagtgct
    31561 tttataacct ctggccagtt aacatctcct tcaagtaaat cacagaatcc atttatattc
    31621 cctattgata atttaaaatc ttttagatgt attcttttaa tcaatttacc gagaattctt
    31681 atccacattt caggaaaacc aataagaagg atgttcccca catcaaaata aacctttaca
    31741 tattcagagc caatttcttc aacaaatctt ttcatttcaa gaggactcaa taaaaactta
    31801 ttccaaacat tttccacaca tataaataca ttattttttt ctgcaagttc aacatacttt
    31861 tttattgatt cctgacttct tttatatgct atatcataac ttacaatttc tccctgtcct
    31921 gatagagaag caacaactcc aggaacaaca agtatagcat ctgttcccaa atagtttgca
    31981 atcttaatcc ctttttcaag taattcttct cctttttttc ttatttcagg gtctgaactt
    32041 gtaagaggat atttccagaa ttgtccaacg agtaaacttg ctatttcaag ccctgttttc
    32101 cttacttttt ctgcaacttt tttaacatct ttttcactgc tgtttaaatc cagaattcct
    32161 ccttctgtag tgttcatttc aacaccttca aaaccggcat ccttaagcat ttttaagcca
    32221 tcttcaaaac tgacattgcc cgggataatc cacatattca cacttttttt catcttttcc
    32281 ctcctttttt aaatatggtc aagtgcagga aaaaataaat caaagggtaa aaatgttttg
    32341 catattcttc ttaatttatc atcaatattc actccaatat cggataaatt aaaaaataat
    32401 ctcgccattt cttcttctct taattttaac acatttttac cttcctgaat aaactttatt
    32461 tctccatttt ccttttttat accaactttt tctttatttt ctatctcaaa gacaatctct
    32521 tctccttctg gaaaattttc ttcaattaaa ggtaaaaatg tttcaattgt ttttttcaaa
    32581 cttactattt ttatcatacc agtatttgaa atgttccagt ttgagcatgt cttaaatata
    32641 atgtcaggta tttcttcaaa atttggaaat tcaagataaa acctactttt cccaaatctt
    32701 tctgataaat accttaaaat tcccagaatt atatcaggtt ctcctccaaa ctcatatact
    32761 tttgtttcag aaccctgttc atttataacc acataagaaa atttaccatt ttcttctgaa
    32821 taataaagag aaatgaacat ctttttataa atctcattga aataatcaaa atctctttca
    32881 accctgtaat tttttctatt gtatgtatct atgattttca aaagaatttc tttttctcca
    32941 agatatcttt tagtccttac tgaactgatt ttaccttttt caaatcctct tgtggtaatt
    33001 gtaatattta taactttacc tgaattttca tagccaaaat ttatatatct gtgtctgtct
    33061 ccccataaaa ttgaaagggg atatccttct ttttccattt ttttaataac ttcattcagt
    33121 aaaatactca tatatccttt tcctctttct tcataatcag ttgaaacagc cccaattcca
    33181 gcaaatttaa tttttatatt tttttgaatt aaagtaagcg gaaaaattct gacaagagaa
    33241 cagagtttat ttttttcttc tattatcaaa atattattga aatcggtatt tttttctttc
    33301 catgcttgag gataacttaa agggaagaga ttgtaaaagt gtccataaac atcctcaaga
    33361 aatcttatta tcctttcata atcctgttta gaagggtttt ttatttccat ttttcaaaat
    33421 ctcttaccaa taatataata tcataaacat aaacttgtca aaaaaaatga aaatatgtat
    33481 tcttgcaggt gaaaaatcag gagataatta cggtgcttta ttgtgtagaa actttaaaaa
    33541 agaaaagaaa gatattttta tttttgggac tggtggaagg gaaatggaaa aagagggagc
    33601 aaaattaata aaggggtttc ctttcggaca gatgggtttt tcaggtgtac ttaaaaaaat
    33661 ctttccttat atttcattta tgaaaaaagt tataaaagag atagaaaaag aaaaaccgga
    33721 tatcattatt tttattgaca atccgggatt taaccttgaa atagcgaaaa gattaaaaag
    33781 gaaatataaa accttttatt atattccacc taaaatctgg gcgcataatt acagaagaat
    33841 taaaatttta aaaaaatata ttgattttgt tattcctata tttcattttg aggtagatat
    33901 ttacagaaag gaaaatattc catatgttta tttcgggcat cctgttatag accttataaa
    33961 aacagaagtt gaaaaatttg aaaaaaaggg atatattata ggaatcttac cgggtagcag
    34021 aagagaagaa ttaatttata atcttcctgc tattaaagat gtaattaaaa atttgaagaa
    34081 tgaattggat tttgatgtct ggatatcatc attgaatgag gaatttaagg agattataaa
    34141 aaaaatattc aaagaccctg atattgagta taaaatagaa aaggatttgt attatttaat
    34201 tgaaaattca gatgttatac tttcagtttc agggactgta aatcttgaag ttgcatattt
    34261 aaaaaaaccg atggtggtct tttacagaac atcttttttg aactattttt tatgtagatt
    34321 tattgttaat gtagatttta taagtcctgt taacttaatt ctaaatgaaa aggttgtacc
    34381 tgaatatatt cagaatttca ggataaaaga cgttgtaaat gatataatta atctgattga
    34441 aaggaaagat atttataaaa aacagataga gggatatgaa agaatgatag aacttcttgg
    34501 agaaaaaaac atcagtgaaa aaatttgcag gtttatcatt gaaggagtta aaaattgata
    34561 aaagaatatt taaagattag aaactatatt aaaatccact gggaatatat tgtaaccggg
    34621 gtcatccttt ctattcttta cattattttg aattctatat cctttacttc tattattcca
    34681 cttatagaca gaatactttc aggtaaaaaa ataacccttc ctgaaaatat tccacatttt
    34741 attggagtta aaatagaacc gataataaca aaaattaatt cttttcctcc tttgcttgtt
    34801 ttaaaatatt taattgcatt catatttttg acttttttac ttaaaggtat ctgtttttat
    34861 ttcagcaact attttttcag atttttttca acaaaaattc agacagacct gagagataaa
    34921 gtatatacaa aatttttaaa tctttctctt gattttttta cctacacaca aacaggtgaa
    34981 ttaacaacaa gggttgtata tgatattgga cttcttaatt ctgtttttga aatgtttttt
    35041 ccgaatttta tatttccaat ttttcttgta atcagttatc tatcaatagt ttttataatt
    35101 gactggcaga tgagtttagt ttcaattttt atatatccgg taatccttgt tcctgtttta
    35161 cactggacga gaaaattaag aaatctcgga agaataattc aggaaacata cggaaaaata
    35221 ggaaatttta taaatgaaag tattttcgga cagaaaataa taaaagcata caatcttgaa
    35281 gaagaaacat taaaaaaatt cagaaaagaa aatgaaaata ttttcagaac aattatgtca
    35341 ataaataaaa gagttctgtt gataggtcct tttactgact tctgtggcgc agtggcttca
    35401 ggtttgatta tatattatgc aggaaataaa attctagcag gaacacttac agcaggtttt
    35461 ctattccttt ttttctttgc ccttttttca ataataagtc cacttaaaga aactatgcaa
    35521 acatatgcta ctataaaaca ttcaagttca gttttaccaa gaatttttta tatacttgat
    35581 ttcaaatcaa ctgtaaagga tgagggaaag gaaatttttg atggtttgaa ggataaaatt
    35641 gagtttaaaa atgttggatt ttcttacaat tcaagaacag ttcttaaaaa tattaatctt
    35701 gtcgttaaaa aaggtgataa aataggaatt gttgggaaaa ccggttcagg caaaagtact
    35761 ttaataagtt taatattgag attttttgac cccacagaag gagaaatttt gatagatggt
    35821 aaaaatataa aggattttaa actcaattca ttgagaaaac atataggtta tgttccgcag
    35881 gaacccatta ttttttatgg tacgataaaa gacaatatta cttttggaga aaataatgat
    35941 aaaagattta aggaagttat tgaaacagtt gggcttttaa aatttataaa tgaattacct
    36001 gaaaaagaaa atacacttct tggagaaaag ggaataaatc tatcaggagg tcagaaacag
    36061 ttaatatcaa tagcaagagc aatatataaa aatcctgaaa ttcttatatt tgacgaagca
    36121 accgcttctc ttgacagtga atcagaaaag atagttcaaa aagcaataga aaaaacaatg
    36181 gaaggaagga ctgtttttat cgttgcacac agattatcca caataaaaga tacaaataag
    36241 attattgttt tggaaaaggg tgaaatagtt gaagaaggaa cacattctca attgtatgaa
    36301 aagaaaggtt tttactataa atttttaaca ttacagcaac tataacttat ctttctggca
    36361 attcaaattc cttaaatcca attttaaaag caggtgaatt attttttaat ttaaagttga
    36421 aattttttat gtcataaaat aaagggtctt ctaaaataga atctgtatcc atccctttct
    36481 tctgccaatc cttaaaagtg taaatcttcc ctccatggtc aaatattttt atttcttttc
    36541 catcaattct gaaataaagg ttattgttaa acataaaatt aaaatcaatt cctccgtaaa
    36601 tccatttttc tgtatttcca ataacaatgt ttctttcaaa tgtaaatctt atatgatttt
    36661 caggtcttgt tatctgtatt tgctggtctc ttccaaaggc aaaaatattg tttctcacaa
    36721 tattctcttt tccgtaatgc tggtggaatc caccatgtgt tgtattataa acgagattat
    36781 tttctgcaat tatatgggta cttccttcat caaaataaat accccatcca ccataccagt
    36841 atccataaat atcatggaag atattgttac ttataagagt tccatcctga attccaagtg
    36901 tatatattcc acccatatca cttaaaattg gaccatctcc atctgttttt ttaccaatat
    36961 gatgaacatt attaaattta acaatatttc cacctgcaag cgattttcca tagccccatg
    37021 tccatccaat acttattcct gtataataaa aatcatatat ctcatttttc tcaataatat
    37081 ttcgataact ctgtccaatc catatcccta ctgcactgtg aaaaatcttt ccgccatcct
    37141 ttataataca tccttttatt tcatttccat atgtctttaa atttttttcc tctctagtaa
    37201 ttctttcacc tatctttatt ccgccagcac cgatactatg agttttacag ttaataattt
    37261 tgttattctg acatcccttt gaaaattcaa acccataagt gccaacattg gttatttcac
    37321 aattttcaaa tttacaattt ctcatacctt cacagtaaac tgcaccggga atttcaattg
    37381 ccgcctgtgc aaaaccacca gattcatttc ctttcaaact tttttctgga aaataccatt
    37441 cagcatgtga aaatgttata ttataaaaat aaatattttc tacatattca tttttttcag
    37501 gacaaccttt aattattaag acattttcaa attttggaat aataaactca taattttttg
    37561 ttttttcttc aggataaaaa tataattttt cactttcaaa atcaaaatac cattctttct
    37621 catccataaa ttcaaaaata ttttcaatgt aataaaggtc atctttctgc ggtttaaata
    37681 cagttctctt tttaaactca ataatccctt tatttatatc cacataataa actggtaatc
    37741 ttgattcaac ccacctcgtc attaatatta cctcactatt tttaaaatta ttccagtttt
    37801 ttatatcttc attatgaatt ttcaccttat atacaccttc attccattct tttttttcat
    37861 cttcttcaga aattcccgca attttcagat aaccgttctt aggatacctt gctcttttca
    37921 aatattcatc atttacccat aaacttctga atttttttcc tttaacatca aaaacaaaaa
    37981 actcttttcc atcaatcttt tctgttttgt aattttttaa cttctcacca gcactaatta
    38041 taactctttc atttttataa ggcattataa ttacaggtaa atcagaagtt cctgagtgtt
    38101 catacgttaa ttcaacaggt ttttcaagaa agtatatacc accttttatc gtaatcacta
    38161 cctgtttttt aagaattcca ccatttttct gtttaatctt tattatttcg tcaactgctc
    38221 tctgtaaagt tttaaaaggg ccatcttttt tacttctttc ctttaatcta ccagaaaagg
    38281 aattatctcc ttcatttgaa acatataaaa tttcagcatt tttaaattcc attttatcct
    38341 ccatttttcc agaactcttt tctatcagga atataaaaac aaatatcaaa aaaatacaga
    38401 taaatttttt catcctatct tttttatttt aaataaaaaa tcagtaaaag caaaaaagat
    38461 ttaaaattga catattttta agattgaata taattttttt aaatcacagt aaaggagaaa
    38521 acaggatggt tgaggcgaaa aatttaacaa aattttatgg aaagacaaag gcaatagaaa
    38581 atgtctgttt tacagttgaa aaaggagaga tagtaggact tcttggacca aatgcagcag
    38641 gaaaaaccac aataatgaga atattaacct gttttttaac tccaacttcg ggtactgcta
    38701 tagtcaatgg atatgatatt cttgaaaatc cacttgaggt tcagaaaagt gtaggatatc
    38761 tacccgagag aaatccttta tatgaagaat ttactgtaag tgagttttta aattttgtct
    38821 gtgaaacaaa aggaatagtt aagaaagagg aaaaaaacaa aagaatttca aaagttatgg
    38881 aagagtgtgg actttctgaa gtaaaaaata aaacaatata taaactttca aaaggatata
    38941 aacaaagagt tggtattgct caggcacttg tgaatgaacc ttctgtttta attcttgatg
    39001 aaccaactat tgggcttgac cctaaacaga tattgaaaca agaaacttaa taaaaaatct
    39061 atcaggtaaa agaacaatct ttctttcaag tcatattctt cctgaagtaa gtatggtatg
    39121 tgaaagagtt atcattataa atgaaggaaa gattgttgca actgatactg ttgaaaattt
    39181 aacatcaact ctgaaaggag gaattgaaat atatcttgaa attgatggag atgttgaaaa
    39241 agttaaaaaa gttttaggag aaattccagg tgttaaaagc ataaatatta tctcttctgc
    39301 gggagaaaaa ataaataaat atgttgtcat agcagaaaag gatataagaa aagatatttc
    39361 agaaaaaatt cttaatagtg gttttggact tttaggtatg agaataaaag aaatgactct
    39421 tgaagatatt ttccttaaat taacaacaaa ggaataaaaa tgagaattct gtctattttc
    39481 aaaaaagaat ttaaaagtta ttttatttct ccggttgcct atgttgttat tctcattttc
    39541 aatttaattt ctggttttat cttttatgcc ttatgtgcct attattcaat tctttcaaca
    39601 agattttcgg gaagccctta tccctatttt actctttcac caaatgaaat gattattaaa
    39661 cctttatttt ttaatatggc tataacatct ctttttgttt taccgctttt aacaatgaga
    39721 ttgttttcag aagaaagacg gcttggtaca atagaacttt tatttacata cccgcttaag
    39781 gattcagata tctactgggg aaaatttctt gcctgttttt caatctttac tataatgacc
    39841 ttgataccat ttctttatat ttttattctt gaaaaatggg taaacctgga taagggaatt
    39901 attttaaata gttttctcgg acttctttta ctcggaggta gttttatttc tataggtatt
    39961 ttaatttcct ctcttacaga aagccagatt atttcagcaa ttataacttt tggagtttca
    40021 ttacttttct ggatcattgg ttggatttct gaaattttac caaaatattc aaactttttt
    40081 gactttattt ctatatataa tcattacgaa aattttccaa aaggtattct tgatacaagg
    40141 gatataatat tttatttaag tatttgtttt ttcttttcat ttttaacatt gagaattctt
    40201 atttcaaaaa aatacagggg ttaaaatggt tgaaaaaaaa tttttttata gaataaatgc
    40261 ttttttaatg gttttaatct ttcttggaat tcttattttt gtcaatctta tatctgaaaa
    40321 aaattacaaa agaattgatt taacgaaaaa caaaattcat tcaatttctc ctcagacaaa
    40381 aaagataatc aaaaacctga aagaacctgt tgagataatt attttttaca gagaaaaaat
    40441 agatgaaaag acaaaggaac ttcttgaaca atacaggtca aattcaaaat ttataagtta
    40501 tagatttgtt gaccttgaca gggatgtttt acttgccaaa aaatatggaa ttacaagtta
    40561 tgatacaatt ctgataaaaa ctggtgaaaa ttacgaaaag atatactcac ttggagaaaa
    40621 agaaataaca ggtgcaatat taaaactcac aaaaaaagaa aagaaaaaga tttattttac
    40681 aaccggtcat ggagaaaaag gaatagatga taaattgagt aattttaaaa agtatcttga
    40741 agaagaaaat tatacagttg gagaaacatt aatattgaga gatggaatac cagatgattg
    40801 tgatattctt gtaatatgtg gtcctgaagt tgattttgtt gataaagaaa ttgaagaaat
    40861 aaagaaattc attgacagcg ggaaaagact tcttgttttt ctggatacag gtaattttcc
    40921 gaacttaaaa aatttacttg gttcttatgg aattgaaatt gaaaatgaca taattattga
    40981 ccttgcaggt agaagatttc ttggagatgc tttaagtcct ttagtatttg attttcccta
    41041 tcaccagata acgagagatt tttcaaattc agcatgtgtt ttttcctctg taagaagtgt
    41101 taaattgaag gaaaatttac cttctgaaat taaaggggat gttcttgcaa ggacaagttc
    41161 tgcttcatgg gctgaaaaaa atatgaagga aatagaaaaa ggtaaggtta attatgatga
    41221 aaatgatgaa aaagggcctg tttctgttgc tgttttggtt gaaaaagaaa taaaagaaaa
    41281 ggaaggtaat aaaaaagcat gtgttgctgt ttttggagat tctgattttg tcacagataa
    41341 gatgattggt tttttgctta ataaagattt tgcgatgaat acagttaatt atcttgccga
    41401 agaggacatt ttaatttcca taagggaaaa gaaagaagaa aatcagccat taattctttc
    41461 agaaaaagca ggaaaatttt tatttttctt cccagttgta ataattccgg ttttaataat
    41521 aatttcaggt agttatatca caattagaag aaaatttttg tactaaaatg aagatattta
    41581 aaaaaaatct attgcttctt atcattttac tcattgtagg ttctttctat ttttatcagg
    41641 ttaaaattgc ttcaaaaaag aaaaaaaatg aggagaaaaa actttttgtt tttgaaaagg
    41701 agaaaataaa gggtatctta ataaaaaatg atgataagga aatagaaata gtaaatgaaa
    41761 atggactctg gaaaataaaa gggaaaaatt atgagtgtga taaaaacgaa atagaaagtt
    41821 tgataaataa aattctatcc cttgaaatag aaagaaatat tggtacagtt gatgatttaa
    41881 gtatttacgg actttcatct tctgaaaaac aaatagaaat ccagaaagat ggagagaaat
    41941 ttatacttta tattggagac gagacaccat caagtagtta cctctataca acaaaagata
    42001 aaaaggaagt tttactcgta tataaatggg atttagacag tattcttgga aaaaatattt
    42061 ttgacttaag ggataaaaga ataatacctg ttgatataac aaaagaagat attgaagaaa
    42121 ttgaggtgaa caaaaaaaat aaaacaattc tattaaaaag gtcaggagaa aggtggtata
    42181 ttgaaagtcc tttaaaagac attgcgaata gagagaaaat agaaagagtg gttggaaata
    42241 taattgatgg ggaggtaaaa gaatttgaag aattaaaaac tgaaaaggaa tgcggacttg
    42301 aaagacccga aagtacaatt aaattaaaaa ctaaagacag taattatttt atttatcttg
    42361 ggaaaaaaaa ggagaattta tattatgcca aaaattctct gaaaccatat atctttctgg
    42421 ctgatgataa aattgttgat gatatacctg atgaaataaa tgaattgaga gaaaaaaaat
    42481 tgtttgatat agatgtttca gaagtggaag aattttcaat cataaaaaaa gataaagaat
    42541 tgaaatttaa aaaagaaaag gattcttatt ttcttgaagg tgaaaaaaca aaaaaaattt
    42601 caaaggataa ggttgaggaa ttccttcatg acttaaaata tcttgaaatt aaggatttta
    42661 tagaatattc agaaaaaaac ttaaaagatt tccaactttc tccaccttta ataaaaataa
    42721 ttgtttatac agaaggaaca aaaacagaag ttcattttgg aaaaaagaca gaaaattata
    42781 tatattgcta tagcccagaa agaaagagta tatttacaat tccttcttct gattattcca
    42841 gaattgataa agatgaggga ttttttgttg aagagaagaa aagaaaaaat gaataaagga
    42901 ataaaaactt taattttttt agttcttatc tttataattt tcttttcctt ttttaaaatt
    42961 tcaaaaaagc aatctattcc agaaataacc attataggag gagaaataaa agagggagat
    43021 ataattggaa atatttttaa aacaaatggt ttatcagatt atgaagcagg aaaaattact
    43081 gaaaaattaa gagaaatttt tgatgttaga aaaagtaaaa taggtgatag atgggaatta
    43141 catttttcat taaaaggaga ttttttaaag tttaaatatt tcagtaagcc atttaatttc
    43201 tacatcgttg aaaaaaaaca agatacaggt gattttattt cctatacaga agaaattgaa
    43261 aagttcaaaa aaacatttac aaaaacagga tttatcaata attctcttta tgaaagtatg
    43321 ataaattcaa acataaatcc tgaaataatt atacagtttg ctgaagtttt tgaatcaaaa
    43381 attgactttt tcactgaatg ccagaaagat gataaatttt caattatatg ggaaagttgg
    43441 gcagacaaag atggaacaat tttaaaagac ataagagttg tcgctggaaa atatgaaaca
    43501 agaaaccaga aatattatgc tttttatttt gaaaatggaa atttcagtgg attttttgat
    43561 gagaatggaa aagggcttga aggttcattt ttaaaagcac ctttatcgta tagaagaata
    43621 acttcttttt tctcctattc aagatttcac ccaatttaca aagtttacag accacatctt
    43681 ggaatagatt attcagcacc aacaggaaca cctgtttctt ctattggaaa cggttctgta
    43741 atatttgccg gttggactaa taatggatat ggaaaatgtg taaaaataaa acatccaaat
    43801 ggttatattt cttattacgg acatttaagc agaattgaaa aaggtataaa aaagggtgta
    43861 aaagtaaaaa aaggagaagt aattggttat gtaggaatga caggaattgc aacaggtcct
    43921 catcttgatt tcagagttca gaaagatggt aaatttataa actttttgaa gttaaaattt
    43981 gtacctgaaa aaaatatacc aaaagaatat atggatgaat ttgaaaatct gaaaaaaaga
    44041 tatatggaac ttttaggttg aattttgtaa aaaataagat ataattaaat agaacaggga
    44101 tattatttat agtaaacaaa atttataacc gaacttatag actttaaaat caatataatt
    44161 ctttgtggga taatggatta agtcaaattt cagttttcta attagtgttt aatttatttt
    44221 tcagtttcaa ctcaactttc aattttttca acatatcttt ctgcaactga cttatcttac
    44281 ttatctgtct tataatcaat cctttaacat ttatatatcc aatctgtatc tttcttaatt
    44341 gctcaattat gtttttaaaa gtaagtgaat atttcccacc ttttcatcaa ataattcagg
    44401 ataaaaacca ctatctttct cctctactaa tt
//
LOCUS       DAOSAT010000002        39406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_771_length_39406_cov_5.041397, whole genome shotgun sequence.
ACCESSION   DAOSAT010000002 DAOSAT010000000
VERSION     DAOSAT010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 39406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39406
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_771_length_39406_cov_5.041397"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2425
                     /gene="mutS"
                     /locus_tag="PLF90_00195"
     CDS             <1..2425
                     /gene="mutS"
                     /locus_tag="PLF90_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOK55736.1"
                     /translation="NAGKSGKIPMCGIPYHAADSYISKLIKNGYKVAICEQVEDPKKA
                     KGIVKREVIRVLTSGTYIDETNPEARYLFSLFIDENSFGYSFIESGGSVIYTNENQDI
                     TKIIEIIFKLPVYEVIYPESRKDKIKVLFSNPLLKLKNITLTELEDWHFNYDISKKTI
                     LETFKVAGLSGFGIEKLVYAQRSIGPLVDYLKDTNKSNLLHLDRISLYNDNEFVYLPS
                     CAIKGLEIEDLIKIIDRTNTPMGKRLLKYWVLHPLTDIKEIQKRHEIVEFLIKNKSLR
                     EKIKNILADIRDTEKALTKISYGYGSAKDIIEIKKLLKKTSELLENCEEIFRKFDYFE
                     IDDIPNLRNLLERAIDENIPSVNFEGKVIKKGFNEEIDRLREIKENSENYLAKYQEEE
                     IKRTGINSLKIGYNKIFGYYIEITKPNLKLVPPDYVRKQTLVNAERFTTQKLKEFEEE
                     ILTAEEKIILLESEIIEKIKEEILKNSHKINLINETIAKLDVLLSFTEVAIENNYTRP
                     IVDKSTIIEIKEGRHPVVEKSVEEFVPNDTFLDTIENYLLIITGPNMAGKSTYIRQVA
                     LIVLLAQIGSFIPAKYGKIGYVDKIFTRIGAQDELTKGQSTFMVEMSETAEILNNLTE
                     RSLIIIDEIGRGTSTYDGFSLAWSVAEYLHKKKVRTLFATHFHEITALGKYRGVKNLN
                     VSVKKSGDNIIFLHKITEGATDESYGIYVARLAGIPENVIKRADEILSKLELEGTIKD
                     KIIGEVEVEYPSLFSLEEKNKVDELKEEIKKLESIKNDLKSIEIENLRPIDALLKLKE
                     LKERINGKS"
     gene            2412..4106
                     /gene="mutL"
                     /locus_tag="PLF90_00200"
     CDS             2412..4106
                     /gene="mutL"
                     /locus_tag="PLF90_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOK55737.1"
                     /translation="MGKVNFLTESIISKIAAGEVVESPASVLKELIENSIDAKCRKIE
                     VKIEKSGKKLIYVKDDGEGIESDDIEKIFERYSTSKIKNLEDLYNIKTLGFRGEALYS
                     IGAVSDVILKSKVKNSETGKEIHIRGGKKLSIKDCGMPDGTIVEVRELFFNTPARRKF
                     LKSDITEFRKLLNIFIQYSICFPKIYFILENENKKIINLSYSENYLKRFCEVLNLDEK
                     YLIYEEKKFDDFDVNIIAGDINLKRPQKDMQFIFVNERPVYDYVLSSAVNNFYQSIFF
                     SEFFPVFSLFLKVPSENIDVNIHPAKREIKIKSESEIARKIVELLKEILRKGKVKTIE
                     VEKKYEEAKKELTDEEKIKEKNNIFIETEKNSEELFILEKKEEFLKEKIKKSSFIGIF
                     KFKYLIFEVENNLLIIDQHAAMERIVYEKFIDEVEKGNLQIQQLLTPVIIDLNPEEMT
                     IYEKTEKILEKYGFLTTRWSENRIAIHGYPFLIKDIEFAIRNILGEKDVKKYEKEDIA
                     KRACKVSIMAGEKISEKEARYIVEKLSECKNPFVCPHGRPVVIEISERFLDSQFSR"
     gene            4126..5490
                     /locus_tag="PLF90_00205"
     CDS             4126..5490
                     /locus_tag="PLF90_00205"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOK55738.1"
                     /translation="MELLFRVKTSFMNTRKIIAFILSITFTILGNEFKKLAINENERV
                     IVFAPHPDDEIIGCAGLISSVIEKNGEIWIVYLTNGDHNQITFKVYEKKPILTPSDYI
                     KLGEKRRNESTNATSILKVNKNNLIFLGYPDFGTLKIWKEFWGNTKPFRSFLTRANSV
                     PYQENYSFGKPYKGESIVSDIEKIVKEINPTKIFITPPIDLNVDHRALFNFVNLALLN
                     IYSDSTFPEIYCYLVHFKNWPQPIKYNPEIELLPPDSLKNLFEWYILEIGKEQTEKKY
                     TCLNCFNSQIILKKSWFYSFVRTNELFYRLFYDVIKNNTEIKFESEKEEFENNKKQSI
                     PFSIVMQKNKDEILFILLHRKKLVEKVDYIFYLYPWKKNINFGIMPKIKLKVSTERGI
                     LIDKTLSKKYRIQLNKELDGLNFKIDTKELGNPDYLFFSSEISINDMIHDFIPWRIIK
                     IEKD"
     gene            5477..6517
                     /locus_tag="PLF90_00210"
     CDS             5477..6517
                     /locus_tag="PLF90_00210"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOK55739.1"
                     /translation="MKKINIKFLKNEWQKELKVLKKNPLKVSLSFTTGVFIGFTPTIG
                     FQTILSYLVSRLIGGSFIIMFIGSSIPTGIPYLIPFTYYGCYKVGLLITKTHPVFTLS
                     EFKEIKSFLSWIIIDIGKPLIYGCLICGIIGWIVSFLIIYSILKFRNKKFFSVFLGVL
                     WEWFIIPSFFYFIFKNKKILEYVFPKNKFLSLFIFILYFISGLILTVLSFYSLHKKGK
                     GTIMPVMPTKKLVTDGVYSYCRNPMYLGYSFLYFAFAFLLKNIWFSFISIGIFIFIFV
                     LAKVFEEKKLYERFGEEYLEYRTNTPFIMPLKILCYYRRNSLFYFFYLCFILCIFIFL
                     FNFYLLICLLSK"
     gene            complement(6525..7157)
                     /locus_tag="PLF90_00215"
     CDS             complement(6525..7157)
                     /locus_tag="PLF90_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HOK55740.1"
                     /translation="MESKEDLKKLYKKIKPEIKKRIKEFKDNLNKNIEDIFAELSFCI
                     LTPQSKAEICWQAIETLKKENLLFYGHESEISEKLKKVRFNKKKAKYIILARKKFFES
                     NLKDLIKTCKDIHLLREILVKNIKGFGMKEASHFLRNIGLGDNIAILDRHILKNLKNF
                     RIIEKIPETLTKKKYIEIEKKFINFSKKIKIKPDELDLLLWAKETGKVFK"
     gene            complement(7178..7293)
                     /gene="rrf"
                     /locus_tag="PLF90_00220"
     rRNA            complement(7178..7293)
                     /gene="rrf"
                     /locus_tag="PLF90_00220"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(7349..8218)
                     /gene="xerD"
                     /locus_tag="PLF90_00225"
     CDS             complement(7349..8218)
                     /gene="xerD"
                     /locus_tag="PLF90_00225"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOK55741.1"
                     /translation="MTFEEMIEMFINHILIEKNLSLNTVSAYKNDLYKFKAFIEEKKI
                     NINKLNYEIFTSFIIYLKRKNYSSTSIVRIISGVRNFYKFLVARGYIKNPEISIESPK
                     IEKKLPDVLTEEEVKKLLNVNKISRKHLRNLAILELFYSSGLRISELCNLKISDLNLE
                     SGFVKVRGKGNRERIALLNNITTEIIKEYLNQRKTVDNEYLFLNNQSKKISRQSVWKI
                     VKKYAKYAGIEKNVKPHTLRHTFATHLLERGLDLRIVQELLGHKSIATTEIYTHLNRK
                     KIKEIYKKYHPRS"
     gene            complement(8215..10212)
                     /gene="ligA"
                     /locus_tag="PLF90_00230"
     CDS             complement(8215..10212)
                     /gene="ligA"
                     /locus_tag="PLF90_00230"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOK55742.1"
                     /translation="MAKTPKERIEELVKLINYYNYMYYVENNPVISDYEYDKLYKELV
                     ELEKKYPEYKLPDSPTQKVGGQPLREFKTIEHKIPMLSMDNTYSEEELIEFDAKIKRM
                     ADVKEVDYVVELKYDGVAVSLHYEDGEFILGVTRGDGFRGDDITENLKTVKTLPLKIK
                     YKESIEVRGEVYMRKDDFEKLNRERKEKGEVLFANPRNATAGSLKLLDPKLVAQRNLQ
                     IFIYQGFLNNGPNTHWEVLNFLKEIGFPVSPYRKCCKNIEKVIEYCNEWREKRFTLPY
                     NIDGMVVKVNSLDLQRTLGTTTKSPRWAVAYKFPAEQVSTIVKDVIVQVGRTGTLTPV
                     AILEPVFVSGTTVSRATLHNFDEIKRLGVKIGDRVFVEKSGEIIPKVVKVIPEARTGK
                     EKDIPVPETCPVCGSRVVKDIQEVAIRCPNIRCPAQVKERIIHFTSRDAMDIEGLGEK
                     WVNILVDKGLLSDYGDIYYLKYDDLIKLERMGDKSVKNLLEAIDKSKTRPFSNLIYAL
                     GIRHIGVHASEILSDEFNSIDELKNATIEKLSSISEIGPIMAESIVDFFSNEENLKVI
                     EKLRKAGVKMEKEKTEEKKDILSGLTFVITGTLKNYSRNEIQDYIKKLGGKVTDSVSK
                     KTDYLICGSEPGSKLQKAQQLGIKIITEEEFEKLVKERMNK"
     gene            complement(10231..11340)
                     /locus_tag="PLF90_00235"
     CDS             complement(10231..11340)
                     /locus_tag="PLF90_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HOK55743.1"
                     /translation="MERNITELPLHFGSAPKYLFERQKKILFEIGRLIIMEFGTEEFL
                     KRLSDPYYFQVLGCISGFDWHSSGVTTTVCHALKNVFNNNPEYGIFIFGGKGKNGLLT
                     PEEIRNNRFIDRKDEIIFISKLTSKIDNNCIQDGYQLYHHNIFVDKKGNWAVIQQGMN
                     LKNNYARRYHWFNKITSSEDKKLVIEPHSGIISVKKESYVLNLIDKRSKEVQNNLVDF
                     LKSESPDSLIKEFEVIFKKSSSIFDRGKKLILPSHHHVEYNFDTKRLYRNFCKLKEKD
                     IKSFPDIVLTENIGIKTLRALCLISEVIYGNSVSFNDPAKFSFAFGGKDGHPYPLDRK
                     NYDITINYLKNIIDKAKIEGKEKLEILKKLQHLYK"
     gene            11427..12281
                     /locus_tag="PLF90_00240"
     CDS             11427..12281
                     /locus_tag="PLF90_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOK55744.1"
                     /translation="MEKLLSIYNEVYKIINSVSENKNEITEINLFNEKRKAGILFLPD
                     FCSSVLIILPGAGYNEKFQKSVFKNFKFLLKKNIGICIFNLNNGNLDKEDVIFFNYFK
                     EKIGEIRGIIEYIKNELKIKNVNLLGISFGGILGFIVAALEKEIKKSIFILSGVNLEF
                     ITWKSLLRFRIKKDCKRVVCKRMHKVYRNLLKNNLYNEILNLPRKCFLYDPFTYLTNL
                     IDREILMINSIFDMIIPFYCVLEVKKRLKNVKTLWYPSTHLTLKFFIPFIRKNILNFL
                     KNENISGN"
     gene            12259..12600
                     /locus_tag="PLF90_00245"
     CDS             12259..12600
                     /locus_tag="PLF90_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOK55745.1"
                     /translation="MKIYPGIDLIEIERFEKAYQKYGEKFLKKIFYNDELLYLEKNII
                     KMCISFSFKESIWKSLPEKIQKKLLFKDIKIGWDNNNPFLLKKIENYEILLSYSITEK
                     YIITTVLLIEI"
     gene            complement(12583..13779)
                     /locus_tag="PLF90_00250"
     CDS             complement(12583..13779)
                     /locus_tag="PLF90_00250"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003368497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOK55746.1"
                     /translation="MKILVINCGSSSIKFKLFEMPEENLISYGSVEKIGEEISIFKYS
                     GKIKIEKQLKIETHQKGIELIAKTLLENEIEDIREIKGIGHRVVHGGEGFEKSVEVDE
                     NVIKKIEDYIFLAPLHNPHNLAGIKGCIEIFPESIQVAAFDTAFHTTIPKIANLYAIP
                     YSFYEKHKIRKYGFHGISHRYVARRTSEIMGKGKYDINVITCHLGNGCSVTAVKNGRS
                     FDTSMGLTPLEGLIMGTRCGDIDAGVILYLLEGLNLKISEVSEILNKKSGLLGISEIS
                     NDFRNLLPLYNKNEKVTLAIDMFCYRLKKYIGSYMAVLGKIDAIVFTGGIGENVPLVR
                     KKSLENMEFFGIIIDDEKNEKVFGKEGEISKDNSLIKVFVIPTNEELRIAFDTYQIIL
                     NSNLNQ"
     gene            complement(13779..14024)
                     /gene="acpP"
                     /locus_tag="PLF90_00255"
     CDS             complement(13779..14024)
                     /gene="acpP"
                     /locus_tag="PLF90_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOK55747.1"
                     /translation="MNDVEIIERVKKVISEVLGVDTDKIRLEDRFVEDLGAESVQSLE
                     LVAAFEEEFGIEMDEEKALEVKTVAKAVDFIKEYLKK"
     gene            complement(14033..15295)
                     /locus_tag="PLF90_00260"
     CDS             complement(14033..15295)
                     /locus_tag="PLF90_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOK55748.1"
                     /translation="MKILIYEDENWINLTPISYLKAVFELRCGFNSILEKIISILPEG
                     EVILWVRDYLKELCKEKFNYKVNEEDFLKDDVLIVNGRWLIDRRVELNLKEERVYLSN
                     GEIVYGLLKKESVNKYWNGNINDFLKILISNVKKEDTDFTIIRFPWNLVHHNSKLIKF
                     EFNKINKRGISGKFSEKACVYGSEKDVFVAQTAEVYPFVVIDASEGPVYIDEGVKIFP
                     FSVIIGPSYIGEKTQIMPGAKIREGNSFGPVCRIGGEVEESIFHSYSNKYHDGFIGHS
                     YVGEFVNLGALTTNSDLKNDYSNVSVYLNGKPTDTGELKVGSFIGDHTKTSIGTFFNT
                     GTIVGIMCNITADNILPKYFPSFVWFVSGKFMKGYGVDYSLKTAKIAMERRNRILLSS
                     EETVIRKVYEMTEAERKIHIEKSRKISK"
     gene            complement(15311..16285)
                     /gene="pta"
                     /locus_tag="PLF90_00265"
     CDS             complement(15311..16285)
                     /gene="pta"
                     /locus_tag="PLF90_00265"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOK55749.1"
                     /translation="MDKIQEFIERVKGLNKKVVFPEGEDERILLSASELKKERIVNPV
                     LLGNKEKIFQTGENLGITNLSEIEIIDPENSELFGILVDRYCEKRKNINKNVAEKIVR
                     RPLIFGGLLVDTGNCDSMIAGATNTTANVIQSAALTVGYLEGIKTTSSFFIMVLPDGK
                     IYFFADCAVNISPDENQLAEIGITTGLNYKKLMGENPKIAFLSFSTKGSASHPLVEKV
                     QKAVKIAKEKNREFDIDGELQVDSAINEEVAKRKVKEFSPVAGKANVLIFPDLNSGNI
                     AYKITQYLGNAQAYGPILQGFSKPVSDLSRGAKVKDIIVTTVITCLMS"
     gene            complement(16307..17101)
                     /gene="nagB"
                     /locus_tag="PLF90_00270"
     CDS             complement(16307..17101)
                     /gene="nagB"
                     /locus_tag="PLF90_00270"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOK55750.1"
                     /translation="MRVIITKSYEEMSKEAAKIVKDLIKKKPNCVLGLATGSTPIGLY
                     QELIRIHKEENLDFSNVITFNLDEYYGLSPEHPQSYRYFMDVNLFNGINIKKENTHVP
                     DGKISIDKIEEYCKSYEEMIKKAGGIDLQVLGIGGDGHIGFNEPGSSIYSRTRLVALD
                     EQTIKDNSRFFEKVEDVPRFALTMGVGTILEAKEIIFLANGIKKAEVVAKAIEGPITS
                     QITASVLQLHPKVTVILDEEAASKLSRKEYYRFAYEAERKIGIKLF"
     gene            complement(17073..18434)
                     /locus_tag="PLF90_00275"
     CDS             complement(17073..18434)
                     /locus_tag="PLF90_00275"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOK55751.1"
                     /translation="MVKENVNKVLEEKIASFKPGSLIKGRVVDILNEEVIIDIGFKSE
                     GVTSKEEFKGRGNELKKGEEVYVVIESLDPDSNGLIPLSKEKADIMLYWEQLEEKFKN
                     NVPVEGVIFKRVKGGYRVDIGVTAFLPSSQTDIVPIKNPNEYVGMKTFFKILKFDSLR
                     KNVVVSRKQYLEEEKERQKIEYLKSLSKNQLVKGVVKNIVDYGAFIEIEHEVVGLLHI
                     NDMSWGRISHPSQLLSIGEKVEVVVLDINFEKQVVSFGLKQKTQNPWDKVEEKYPVGS
                     IVEGKVVNITEYGVFIKLEEGVEGLLHISELSWTGRIKHPSEVVATGDTLKVKVIDIK
                     KEEQKISFSLRQLEPNPWPEIEKKYPVGTIVTGKVYHITDFGAFVEIEKGIDGLLHIS
                     NISDTPIKHPSEVLRKGQKVELMILEIDPENKKISLGMKQLGESFKDKKRRKDESDNN
                     EEL"
     gene            complement(18435..19295)
                     /gene="ispH"
                     /locus_tag="PLF90_00280"
     CDS             complement(18435..19295)
                     /gene="ispH"
                     /locus_tag="PLF90_00280"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOK55752.1"
                     /translation="MKRLIVAKNIGFCFGVKRAVETTEKLLEKLKSLSTVGDIVHNPL
                     VMENLKLKGLKVFNSVSEIKGKYFLIRSHGLAKKEIEKLKKSNLEIYDMTCPFVKKIH
                     YLVENLSNTGYNILIVGNKSHPEVLGIKGYGRNIKVIESKEDLLKYKYFRGKKVAVVS
                     QTTLNFNYYFEIVKDIVKMVESNKILVLNTICKITEEREKEGVEIAKSSDAVFILGGK
                     ESSNTKKLFKICKKECKNTFFIEKFADLSKIKFSDFKNIGIVSGTSTPNFFIRKVIEY
                     FKKIGYKEVP"
     gene            complement(19292..19879)
                     /locus_tag="PLF90_00285"
     CDS             complement(19292..19879)
                     /locus_tag="PLF90_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOK55753.1"
                     /translation="MRSFLYLLAKLIGFFVYNVFFLIEIRGKENFPKKGGFIVASNHL
                     SYLDPPTIGFVCPRKLYYFAKSSLFEIKVLSSIVRILGAIPLDRESSMPLTLKKAIKI
                     LKKGEGLVIFPEGTRSKDGLIKEGKPGVGFLAIKSNVPVVPVRLKGTDKALPRNSKFI
                     RLKKIEVIVGNPVYFEKESYEEISEKVIEIIKTLK"
     gene            complement(19876..20229)
                     /locus_tag="PLF90_00290"
     CDS             complement(19876..20229)
                     /locus_tag="PLF90_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55754.1"
                     /translation="MKEKIFEKKKFYEKLIKINPDFLEVLFQLSHIYIYQRDYKKVLK
                     IDKKIAELLNLDPISYYNLACDYSLLGDMEKAVMNLKIAISLGFNRKSYIEKDKDLEF
                     LKKSKRFENIKKLIK"
     gene            complement(20405..20665)
                     /locus_tag="PLF90_00295"
     CDS             complement(20405..20665)
                     /locus_tag="PLF90_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55755.1"
                     /translation="MKDYRNKKIVIDTSTTWIYIGTLIDEDNDYFYLEDADDFDISET
                     SLSKQEYLMMVKRDGVVPNRKNIKVLKSKVVGISLLKDILEK"
     gene            complement(20662..21099)
                     /gene="dtd"
                     /locus_tag="PLF90_00300"
     CDS             complement(20662..21099)
                     /gene="dtd"
                     /locus_tag="PLF90_00300"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOK55756.1"
                     /translation="MRLVIQRVSKAKLFVEEKEKGEIEKGLVILLGIGKGDTEDKIDY
                     VIKKILSLRIFEDENGKMNYSVSDICGDLMIVSQFTLYGDCTGGNRPDFTMAEKGEIA
                     EKLYNKFIEKIKKSNVKIVEGEFGKKMTVEIHNDGPCTIILER"
     gene            21160..21960
                     /gene="dapF"
                     /locus_tag="PLF90_00305"
     CDS             21160..21960
                     /gene="dapF"
                     /locus_tag="PLF90_00305"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOK55757.1"
                     /translation="MEIKFFKIVGSGNDFVIIDNRKNLIKNRNSLALKLCDRKFGIGA
                     DGLLLLENSEKADFKMRIFNPDGSEAEMCGNGLRCILRFISENSISKKKHLKIETKAG
                     LLDGIIKGKTMKVRMNIIGEPKLNVEIPVDKSKIIGNFINTGVPHTVIFVDNVEKVDL
                     EKIGPQIRYNKIFGKSGTNVNWVEIIKEGTIKIRTYERGVEGETLSCGTGSVASAIIT
                     SIIKKFKSPVTVITKSKELLKVYFDEDFNKVYLEGKTFYAFKGVWVES"
     gene            21957..24059
                     /locus_tag="PLF90_00310"
     CDS             21957..24059
                     /locus_tag="PLF90_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOK55758.1"
                     /translation="MNIEILILFSFVLGIVLNDFLPFYYIFSLLPLFFISEINRKFII
                     IFLLFFLSGYYITENYKRKLIPEKIDFSQNSVIEGEIINIKEKKFEREIVLNIKKIYK
                     DNENKIWKGKVLLKTRNNDKLLPGENLRIKEFSISKIYPPKNPSEFNYKKFMERQGIF
                     YLIKSDSIEKTESRKNLRLLNSYIREKIEKRIENYMKFNPDGYEIVKLITIGSDDPPD
                     FLKEIGIKSGIYHLFVISGIHIIFLILFFKILFIPFQKINNTHPKFFPFLLLFILWFY
                     DFLCGFKIPVTRAVLMVSFYLIFEIFERKIEPIKSIILAGFLLLFINPYNIYSISFLL
                     SFLSTFGILIIPGKIKLKRNFITNSFISTFSAQLFILPVLFYNFGFFYPFGILNNLLF
                     TPLVGFLTINSFISIFLPFLFFILNFATNIFLKLLIFISNFSLKVNVYFPLFFILIYY
                     FSLFIIFVPLKKSLRFITSFLIILLLVFFQVNLKENKKEEVIFFSLKNPFILINKESK
                     GILITSNKIENPVFYKSTLYKLLKEKKIKIEKAIITGKNFSENLIFISKYCKNIYISD
                     SILKPCFFNYENIKSFHNDDKIITDNFLFSFKKGNLLVKYDNFKFLIILDEDLEKSIT
                     EDKYFLIYLVAYKKNNKNDEIINSLQSPFLILQKETKKFENLKSLCQNYHLNKSSIIL
                     NLKTGMINYWRTNDIRKY"
     gene            24040..24360
                     /locus_tag="PLF90_00315"
     CDS             24040..24360
                     /locus_tag="PLF90_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55759.1"
                     /translation="MILENIEKIKKGEEQSEKIIKDAEKKVKEILETLEAEVEKIKMD
                     KEKIMKEKLEKYKQKKDFEKREKIEFLEKQKEKIEIDLQQKKSKTDEVANKIWEKIKK
                     EIMQ"
     gene            complement(24350..25543)
                     /locus_tag="PLF90_00320"
     CDS             complement(24350..25543)
                     /locus_tag="PLF90_00320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOK55760.1"
                     /translation="MRVLRNFIFLFVILFLLVINWIYLQGKTFIPEFYLISFNIIISI
                     LIVLDIIFAFQILNKIEFLQRENERKDLIINDYKIESTFLSTITEIIETFGEDVTIDD
                     VIGKILDTIKNIFKEEIIVITLFGDRYKMSVKGEHIEISQELIEELAIKGRPVLVNNI
                     SSFSQYGVLAENGIKSFIVCGLYQKKTVIGILGIFSKREKRFTLRDLNLLRMVSVPIS
                     LMIENTELFNKTKILSIMDSLTQLYNRRHFEKLFSEILTKSRVNYKPVSIAMCDVDYF
                     KFYNDTNGHLAGDYVLKTIADILKKGVKGSDIVCRYGGEEFIIIFPDTTKENTVKICE
                     KLRKTIREFKFLNEESQPNKDLTISFGIASFPEDGMTTTELIKKADTALYKAKELGRD
                     RVVTA"
     gene            complement(25554..25904)
                     /gene="rplT"
                     /locus_tag="PLF90_00325"
     CDS             complement(25554..25904)
                     /gene="rplT"
                     /locus_tag="PLF90_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOK55761.1"
                     /translation="MPRTKNSPSSRQRRKKVLKRAKGYRQGRSKLFKRAKEFSEKGLT
                     YAFMHRRKKKRDFRRLWITRISAACKANGISYSKFIAGLKRLGINLNRKTLSDIAFNH
                     PENFASLVNQIKGE"
     gene            complement(25927..26127)
                     /gene="rpmI"
                     /locus_tag="PLF90_00330"
     CDS             complement(25927..26127)
                     /gene="rpmI"
                     /locus_tag="PLF90_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOK55762.1"
                     /translation="MGKLKTKKSVAKRFKITKTGKVIHYGAGGSHLLSKKSSKRKRRI
                     KKSQVLKNKGDIKMVKRLLPYR"
     gene            complement(26114..26665)
                     /gene="infC"
                     /locus_tag="PLF90_00335"
     CDS             complement(26114..26665)
                     /gene="infC"
                     /locus_tag="PLF90_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOK55763.1"
                     /translation="MAREGKERRYRINYQIRSPKVRVIDERGNQLGILDIKTALDIAK
                     EKNLDLVEIVPDSNLPVCRILDFKRFLFEQKKKEKEIKKKQKTLQTKEIRFTPETSEH
                     DYKFKKQHIENFLKEGHRVKVSVFYKGREILYKERGYALLERLIRELSEYGKIDKAPK
                     LEGVRLSVTFIPIDVKGEKNGKT"
     gene            26813..27796
                     /locus_tag="PLF90_00340"
     CDS             26813..27796
                     /locus_tag="PLF90_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOK55764.1"
                     /translation="MKNKIFAFLFIINIFCFSKTQFINRTEFLLDTVFNIKIEKIENA
                     DIILESAFERVKELEDKLSIFKEESEVSRLNKYKKFKVSSELMEVIKKSIYISEITDG
                     AFDITCKKLIDLYRKKNKQNSLPTKEEINKVLKETGWEKIKIKDNEIYLSKNAEIDLG
                     GIAKGFIVDKVFEFLKSKGVKNGIINAGGDIYCWGMNQENKKWKIGIENPFKEGEIIG
                     SFEITDKGVATSGNYKRYMKIKNKKIGHIINPKTGLPVENFCVSVTVISSDCTTADGL
                     ATAIFVLGIEKGLKIFEKYKNFECLIIDKDKKIYKSQNFPFNPYIFSSLNK"
     gene            complement(27769..29031)
                     /gene="lysA"
                     /locus_tag="PLF90_00345"
     CDS             complement(27769..29031)
                     /gene="lysA"
                     /locus_tag="PLF90_00345"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOK55765.1"
                     /translation="MEEKIKEILEPYYKFRKGSFYIEEVEGRKIVEKFGTPVYVYSHS
                     FLKNQINRIKNAFSNLPVKICFSVKSNSNISILKIMKANGLGADIVSEGELRRALIAG
                     FSPSDIVFAGVGKKEEEIEFAVKKNIFCFNVENEEELDIIEKFGKKYGKKVVVNLRLN
                     LDIDVDTHHYIKTSKSENKFGIDIDISERILKKKFNFVEIKGIHFHLGSQIKEVSPYI
                     KAIEKVREFCKKNEFNLEILDIGGGFGIPYKEEDRIQSVEEFGKEIYQTLKDLNLKTL
                     IIEPGRFIVGNAGVLITKVLYVKHKHTKNFIIVDAGMNDLIRPSLYGSYHLIYPLKLK
                     NGKKIKFDIVGPICETGDYLGKDREFPEDIKRNDFLVVMSAGAYGFSMSSNYNTRLRI
                     PEVLVTGKNVKLIRKRETYSYLFKLEKM"
     gene            complement(28956..30380)
                     /gene="argH"
                     /locus_tag="PLF90_00350"
     CDS             complement(28956..30380)
                     /gene="argH"
                     /locus_tag="PLF90_00350"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOK55766.1"
                     /translation="MRYMWEGRFKEKVDVDIIEFTKSINVDKKLAIYDIEGSIAHVKM
                     LFKVGLITEEEKEKIIRGLENIKKEIKENKFKFLPSDEDIHTAIERRLIEIIGKIGEK
                     LHTARSRNDQIVLDEKLFLRDEVIYLVKLIIDLQEKFLEKSEEYFDTIISAYTHLKQS
                     QPILLSHYLLAYVEKLERDKERFMDTYKRINVFPLGVGACAGTSLPIDREYTAKLLNF
                     PEISRNSLDTVSDRDFLVEVTFCCLLTMIHLSSFSQDIIIWNMDEINFVKLPDKYCTG
                     SSLMPHKKNPDVFELIRGKTAVCTGLLNGLLVLLKDLPLSYNRDIQEDKKILFEILKT
                     VEATLKILIKILSEIKFNKEIIIEKISSFTLSVDIVEYLVKKGIPFREAHRIVGNIVK
                     YCIEKNKTFFNLTLNEFKSFSPIFEKDILQILNFKNSVNSKISGGGTSSINVKKEIER
                     WKKKLKKSWNLIINLEKEVSILKK"
     gene            30451..32079
                     /gene="topA"
                     /locus_tag="PLF90_00355"
                     /pseudo
     CDS             30451..32079
                     /gene="topA"
                     /locus_tag="PLF90_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
     gene            32155..32328
                     /locus_tag="PLF90_00360"
     CDS             32155..32328
                     /locus_tag="PLF90_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HOK55767.1"
                     /translation="MNEIIIEDKRCPLCNGQLTLIKGKYGIFIGCSNYPNCTYTERIK
                     ENADNSGNRSKKR"
     gene            32291..33151
                     /locus_tag="PLF90_00365"
     CDS             32291..33151
                     /locus_tag="PLF90_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOK55768.1"
                     /translation="MLIIPAIDLKKGEIVRLYKGRFDKISYYDVEIENVTKEYLKAGA
                     KRIHIVLLYGAKTGKIENEEIEKIKNIIELKKINNRDDCELQIGGGIRKREQIEKFLN
                     LGINYVIIGTAFLIPIALSEGYSIQDIRFFYQKSGKNLEVEKEIPEFDLIDWLEPEIK
                     DKIIISVDYVGDEIALSGWEVTLPLKPSYVIKRFIEKGFKRFLITSIESDGTLDGIDF
                     KNISRITKEITRFKEDIKEIIVAGGISKEEDIIMLNSLQYRPTGIIIGKALYQKKLDL
                     KNVIEKYQKI"
     gene            33123..33686
                     /locus_tag="PLF90_00370"
     CDS             33123..33686
                     /locus_tag="PLF90_00370"
                     /inference="COORDINATES: protein motif:HMM:NF036527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOK55769.1"
                     /translation="MLLKNIKKFSLFLILFFNLVFAQFKANVTPLLNENYSKSILNFI
                     KRAEKSIYVIMFQTGYYSEYAESISNQILREIVNAYKRDVKVEVILDLSTMSNVKEKN
                     FETAKFLAENGIKVYFDKADKTTHSKLLIVDDKYVFIGSHNWNYYALEKNNETSILIE
                     SEETARVFINYFNEVKKECRIFLAPLN"
     gene            complement(33683..34957)
                     /locus_tag="PLF90_00375"
     CDS             complement(33683..34957)
                     /locus_tag="PLF90_00375"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOK55770.1"
                     /translation="MVTVVVGTQWGDEGKGKVIDYLAEKVDIIARYQGGANAGHTVVV
                     NNKKYVFHLIPSGILYPEKICVIGNGVVIDPVSLFEEIEYLKNNGIEVEGKLFIAENA
                     HITLPYHKILDRVEDTFRGKGQLGTTGRGIGTTYTDKFARIGIRVVDFIDDEVFMEKL
                     KIALELKNYLFKEYYKEEVLKAEKIYKDYEIYRKKIKKFVINTSIYLNEEIEKGKKIL
                     AEGAQGTFLDIDFGTYPYVTASNPISGGACIGLGISPKKIEKIIGVAKAYTTRVGMGP
                     FPTEIKDSIGEELRKIGNEFGATTGRPRRCGWFDAVLVKFACMVNGIDEIVLTKLDVL
                     TGLEKIKIGVGYKYEEKIINSYPFNPKLFSNCEVIYEEMDGWKEDISNVKSYKELPKN
                     ARKYIEKIEEKIGTKITKISTGSSRGQTIEKD"
     gene            complement(34951..36033)
                     /locus_tag="PLF90_00380"
     CDS             complement(34951..36033)
                     /locus_tag="PLF90_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOK55771.1"
                     /translation="MSYKITLIPGDGIGPEITEVAKKCIEATGVEIEWDVKMAGEECI
                     EKFGTPIPEDTIKSIEENKICLKGPITTPVGTGFRSINVFLRQYFNLYVCLRPFKIYK
                     GARTKYENVDIVLIRENMEDLYAGIEFEKGKEDTYELIGFIEKKRNVKLSKNTGISIK
                     PISVENSEKIVRFGFEYARKNNRKKVTVVHKANIMKYSDGLFLEVANKISQEYPDIEY
                     EDKIVDNMAMQLVQKPEIYDVIVCPNLYGDILSDLCAGLVGGLGLSPGANIGEDIAIF
                     EPTHGSAPKYKGQNRVNPSAMILAGILMLDYLGETEASDKIERALAEVIEEGKFVTYD
                     FKNPNEECVGTIEMGKAIIKKMGGKW"
     gene            complement(36035..36538)
                     /locus_tag="PLF90_00385"
     CDS             complement(36035..36538)
                     /locus_tag="PLF90_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOK55772.1"
                     /translation="MVLKGKSHKFGDNINTDYIISGKYKFKTLDMDELAKHLMEDIEP
                     EFYKKIRKGDFIVAGINFGCGSSREQAPLVIKHAGIPAVIAKSFARIFYRNGINNGLI
                     LIEGDTDKIENGDELEIYPIEGIIKNITKNIEIKTTKIPDFLIDIIKEGGVISYLKKY
                     KKFKIGE"
     gene            36647..37345
                     /locus_tag="PLF90_00390"
     CDS             36647..37345
                     /locus_tag="PLF90_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOK55773.1"
                     /translation="MNILAIGAHPDDLEILCGGTLAKYSKNGHKIFMAHLCNGNKGGK
                     NISPSELAKIRDEEAKKAASLIKAEVLGPIASDLEIYNTKEMREKVVDIIRYAKPDII
                     ITHSPEDYMPDHTNTAQLVFDAAFTSTLPNYKTEYPAHEHITPIFYMDTLVGLKFQPT
                     HYVDITDFFEIKKQMLLCHESQYKWIKGHHLSDPLNIIETVSKFRGLQCGVLYAEAFR
                     MENVWGRIKPAELI"
     gene            37357..38208
                     /gene="rfbD"
                     /locus_tag="PLF90_00395"
     CDS             37357..38208
                     /gene="rfbD"
                     /locus_tag="PLF90_00395"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOK55774.1"
                     /translation="MKKVLITGGNGLVGNYLNRIFDGKNYSVFSFNKEVLDITNIKKS
                     FEIFKNIKPEVVIHLAALTDVDFCEKNPEKAFLVNSQGTENIAKLSYDFNCFLIYLST
                     DFIFDGLKNSPYSEKDLPNPINIYGKSKLLGEKYIMNNCKKYLIIRTSRIFGKVGKNF
                     SSKLKEIIRERKEIFLTTDVINSPTYALDLSNCIFKLMEMDFCGIINVCNEGWCSFFD
                     FGNKMKEILKLDTEIKGINFDDFSKKYGIIAKRPKFSALSIDLLNSLNIKMDSWETSL
                     KKFLEEE"
     gene            38212..38397
                     /locus_tag="PLF90_00400"
     CDS             38212..38397
                     /locus_tag="PLF90_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55775.1"
                     /translation="MKKVKIGFVGCGGFANMVHYPSLSEIEDVEISAICDIDREKLKN
                     TGEKYKIEKRYTEYKKC"
     gene            38391..39176
                     /locus_tag="PLF90_00405"
     CDS             38391..39176
                     /locus_tag="PLF90_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK55776.1"
                     /translation="MLDENEFDGVYIIMPPYHLYDIVIDALNRKLNVFIEKPPGITKF
                     QTQNMADLAIKNNCKTMVGFNRRFIPLLRKVKEIVEKKGPIIQVVSTFYKNMYPNFLY
                     YNGAVDILTCDGIHAVDTLRWLCGEVKKIKSVIKSYFSNIPNAFNAIIEFEKGATGIL
                     LTNWTVGKRIHTFEIHSLGISAFVDCNDRAIIFKDNREEPEIFSAFDITKSKQFHRFY
                     GYYNENRHFIECIKNDKEPECNFTDAVKTMELVEEIYKNNISF"
     gene            complement(39215..>39406)
                     /locus_tag="PLF90_00410"
     CDS             complement(39215..>39406)
                     /locus_tag="PLF90_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOK55777.1"
                     /translation="GNSHVRRIAFIMAESVRKMTPYFRDYYIKKINEGKSYRQAIIAT
                     STRLLKVIYYLLLDNRLFS"
BASE COUNT        14127 a         5954 c         4879 g        14446 t
ORIGIN      
        1 aaatgcagga aaaagtggaa aaataccgat gtgtggaatt ccttaccatg ctgctgatag
       61 ttatatttct aaattgataa aaaatggata taaggttgct atatgtgaac aggttgaaga
      121 cccgaagaaa gcaaaaggaa ttgtaaagag agaagtaata agagttttaa cttctggaac
      181 ttatattgat gaaacaaatc cagaagcaag atatcttttt tctctattta tagatgaaaa
      241 ttcttttggt tattcattta ttgaaagtgg tggctctgta atatatacaa atgaaaatca
      301 ggacattaca aaaataatag aaataatatt taaattacct gtatatgaag ttatttaccc
      361 tgaaagtaga aaggataaaa taaaagtact gttctcaaat cctctcttaa aattaaaaaa
      421 tataactctt acagaacttg aggactggca tttcaattat gatatttcaa aaaaaacaat
      481 acttgaaaca tttaaggttg caggtttaag tggttttgga atagaaaaac tagtttatgc
      541 tcaaagatca ataggtccac tggttgacta tttaaaagat acaaacaaaa gcaatctttt
      601 acaccttgat aggatttctc tttataacga taatgaattt gtctatcttc catcctgcgc
      661 cataaaagga cttgaaattg aagaccttat aaaaatcatt gatagaacaa acactccgat
      721 gggtaaacga cttttgaaat actgggtttt acatccatta acagatataa aagaaataca
      781 aaaaagacat gagattgttg aatttctgat aaaaaacaaa agtttaagag aaaaaattaa
      841 aaatatactt gcagatatca gggatacaga aaaagcactg acaaaaataa gttatggata
      901 cggttctgca aaagatatta tagaaataaa aaaattattg aaaaaaactt ctgaattact
      961 tgaaaattgt gaggaaatat ttagaaaatt tgactatttt gaaattgatg atatacctaa
     1021 tttgagaaat ttacttgaaa gagcaattga tgaaaatata ccatctgtta attttgaagg
     1081 gaaggttata aaaaagggct ttaatgaaga aatagataga ttaagagaaa taaaggaaaa
     1141 cagtgaaaat tatcttgcaa aatatcagga agaagaaata aaaagaactg gtataaactc
     1201 cttaaaaatt ggttacaaca aaatattcgg atactatatt gagataacaa aaccaaattt
     1261 aaaacttgtt ccacctgatt atgtaagaaa acagacactt gtgaatgctg aaagatttac
     1321 aacacaaaaa ctgaaagagt ttgaagaaga aatattgact gctgaagaaa aaataatatt
     1381 acttgaaagt gaaataatag aaaaaattaa ggaagaaata ttaaaaaact cacataaaat
     1441 aaatttaata aatgaaacca tagcaaaatt agatgtttta ttatccttta cagaagtagc
     1501 aattgaaaat aattatacaa gaccaattgt tgataagagt acaataatag aaataaagga
     1561 aggaagacat cctgttgttg aaaaatcagt tgaggaattt gttccaaatg atacttttct
     1621 ggatacaata gaaaactatc ttttgattat aacaggtcca aatatggctg gaaaatcaac
     1681 atatataaga caggtagcac taattgtttt acttgctcaa attggttctt tcatccctgc
     1741 aaaatatgga aaaattggat atgtggataa aatatttaca agaattggtg ctcaagatga
     1801 actgacaaaa ggacagagta cttttatggt tgagatgtca gagacagcag aaatattgaa
     1861 taatttaaca gaaaggagtt tgataataat tgatgaaata ggaaggggaa caagtactta
     1921 tgatggtttt tctcttgcat ggagtgtcgc agaatattta cacaagaaaa aagttagaac
     1981 tctttttgca acacattttc atgaaattac ggctcttggt aaatacagag gggttaaaaa
     2041 tcttaatgtg agtgttaaaa agtcagggga taatataatt tttctgcata aaataacaga
     2101 aggagcaact gatgaaagtt atggaatata tgttgccaga cttgcaggaa taccagaaaa
     2161 tgttataaaa agagcagatg aaatattatc aaaacttgaa cttgaaggaa cgataaagga
     2221 taaaataata ggagaagtgg aggttgaata tccctcttta ttttcacttg aagaaaaaaa
     2281 taaagttgat gaattaaagg aagaaataaa aaaacttgaa tcaattaaaa atgatttgaa
     2341 aagtattgaa atagaaaact taagaccaat tgatgcactt ttaaaattaa aggaattaaa
     2401 agaaagaata aatgggaaaa gttaattttt taactgaaag tatcataagt aaaatagcgg
     2461 ctggtgaagt ggttgaaagt cctgcatctg tacttaaaga acttatagaa aattcaattg
     2521 atgcaaaatg cagaaaaata gaagttaaaa ttgaaaaatc gggtaaaaaa ttgatatatg
     2581 ttaaagatga tggagagggg atagaatctg acgatataga aaaaatattt gaaaggtatt
     2641 caacaagcaa aataaaaaat cttgaagacc tttataacat aaaaactctt ggttttagag
     2701 gagaagccct ttacagtatt ggtgctgttt ctgatgtcat attaaaaagt aaagttaaaa
     2761 attctgaaac agggaaagaa attcatataa gaggcgggaa aaaattaagt attaaagatt
     2821 gtggaatgcc ggatggaact atagttgaag tgagggaact ttttttcaac acacctgcca
     2881 gaagaaaatt tttaaaatca gatattacgg aattcagaaa attgctcaat atctttattc
     2941 aatattcaat atgtttccct aaaatctatt ttattctgga aaacgaaaac aaaaaaatta
     3001 ttaacttatc atattctgaa aactatttaa aaaggttctg tgaagtttta aatctggacg
     3061 aaaaatatct gatttacgaa gagaaaaaat ttgatgattt tgacgtaaat ataattgcag
     3121 gtgatataaa tttaaaaagg cctcaaaaag atatgcagtt tatatttgta aatgaaagac
     3181 ctgtttatga ttatgtttta tcatctgcag taaacaattt ttatcaatca atctttttct
     3241 ctgaattttt tcctgttttt tctcttttcc taaaagtccc ttccgaaaat attgatgtta
     3301 atatccaccc agcaaaaaga gaaataaaaa ttaaaagtga atctgaaata gccagaaaaa
     3361 ttgttgaatt acttaaagaa atccttagaa aaggtaaagt taaaacaata gaggttgaaa
     3421 aaaaatatga agaggcaaaa aaggaattaa cggatgaaga gaaaataaaa gagaaaaaca
     3481 atatatttat tgaaacagaa aaaaatagtg aagaactttt tattttagaa aaaaaggaag
     3541 agtttttaaa agaaaaaata aaaaaatctt ctttcattgg aatttttaag ttcaaatatt
     3601 taatttttga agttgaaaat aatttgttga taattgacca gcacgctgcc atggaaagaa
     3661 ttgtttatga aaaatttata gatgaagtag aaaagggtaa tcttcaaatt caacaactat
     3721 taacccctgt aattattgat ttaaatccag aagaaatgac aatctatgaa aaaactgaaa
     3781 aaattcttga aaaatatggg tttttaacta caagatggag tgaaaataga atagcaatac
     3841 acggatatcc ttttttgata aaagatatag aatttgcgat aagaaatata ctcggagaaa
     3901 aagatgttaa aaaatatgaa aaagaagata tagcaaaaag agcatgcaaa gtttccatta
     3961 tggctggaga aaaaatatct gaaaaagaag caagatatat tgttgaaaaa ttatctgaat
     4021 gtaaaaatcc ttttgtctgt ccccacggta gacctgttgt aattgaaata agtgagagat
     4081 ttttagacag tcaattttca agatagatta tcttgaaaaa atttgatgga attactattt
     4141 cgtgttaaaa caagttttat gaatacaaga aaaataattg catttatttt atccataacc
     4201 tttactattt tggggaatga atttaaaaaa cttgctataa atgagaatga aagagttatt
     4261 gtctttgcac ctcatcctga tgatgaaatt attggttgtg caggtctgat ttcttctgtt
     4321 attgaaaaaa atggagaaat ctggattgta tatttaacaa atggggacca taatcagatt
     4381 acatttaaag tatatgaaaa aaaaccaatt ctgacacctt cagattatat aaaacttgga
     4441 gagaaaagaa gaaatgaatc aacaaatgca acctctattt tgaaagttaa taaaaataat
     4501 cttatttttc ttggatatcc agattttggt acattaaaaa tatggaagga attctggggt
     4561 aatacaaaac cttttagaag ttttttgaca agagcaaatt ctgttcccta tcaagaaaat
     4621 tattcttttg gtaaacctta taaaggagaa agtattgttt cagatattga aaaaattgta
     4681 aaagaaatta atccaacaaa aatattcata acacccccta ttgatttaaa tgttgaccac
     4741 agagcacttt ttaactttgt aaatcttgca ctcctaaata tatatagtga ttctactttt
     4801 cctgaaattt actgttattt agttcatttt aaaaactggc ctcaacctat aaagtataat
     4861 ccagaaatag aactacttcc tcctgattct ttaaaaaatc tttttgaatg gtatatattg
     4921 gaaataggaa aagaacaaac agaaaagaaa tacacctgtt taaactgttt taactctcaa
     4981 atcattctaa aaaaatcatg gttttattca tttgtaagaa caaatgaact tttttacaga
     5041 ttattttatg atgtcattaa gaataataca gaaataaaat ttgaaagtga aaaagaagaa
     5101 tttgaaaata acaaaaaaca gtcaatccca tttagtattg ttatgcaaaa aaacaaagat
     5161 gaaattttat ttattctttt acacagaaaa aaattagtag aaaaagttga ttacattttt
     5221 tatttatatc cctggaaaaa aaatataaat tttggtataa tgccaaaaat aaagttaaaa
     5281 gtaagcacag aaagaggtat tttaattgat aaaacccttt caaaaaaata tagaattcaa
     5341 ttaaataaag aattagacgg attgaatttt aaaatagata caaaagaact tggaaatcct
     5401 gattatctct ttttctcatc ggaaatctcc ataaatgata tgattcatga ttttattccg
     5461 tggaggatta taaagattga aaaagattaa tattaaattt ttaaaaaacg agtggcaaaa
     5521 agaattaaaa gttctaaaaa aaaatccatt aaaagtatct ctctccttta caactggcgt
     5581 ttttattggt tttacaccta caattggttt tcaaacaatt ttaagttatt tggtttcaag
     5641 attaattggt gggagtttca taatcatgtt tattggttct tctatcccaa ccggaattcc
     5701 atatcttatc ccatttacct attatggatg ctataaagtt gggctattaa tcactaaaac
     5761 tcacccggtt tttactctat cggaattcaa agaaataaag agttttttat cgtggattat
     5821 tatagatatt ggaaaacctt taatttatgg atgtttaata tgtggaataa ttggctggat
     5881 tgtttcattc ctgattattt attcaatatt aaaattcagg aataaaaagt ttttttctgt
     5941 atttttaggt gttttatggg aatggtttat tatcccttct ttcttttatt ttatttttaa
     6001 aaataaaaag atacttgaat atgtctttcc taaaaataaa tttctttcac tttttatttt
     6061 cattttgtat tttatttctg gattaatttt aacagtttta tccttttatt ctttacataa
     6121 aaaagggaag ggaactataa tgcctgtaat gccaacaaaa aaacttgtaa cagatggagt
     6181 ttattcatat tgcagaaatc ctatgtatct gggatattca tttttatatt ttgcatttgc
     6241 ttttctttta aaaaacatat ggttttcttt tatatccatc ggaattttca tttttatatt
     6301 tgttctcgct aaagtttttg aagaaaaaaa attatatgaa agatttggtg aagaatatct
     6361 agaatataga acaaatactc cctttattat gccgttaaaa attttatgtt attatagaag
     6421 aaactcactt ttttatttct tctatttgtg ttttatcctc tgtattttta tttttctgtt
     6481 taacttctat cttttaatat gcttattatc aaaataaaat tttttcattt aaaaacctta
     6541 cctgtttctt ttgcccataa aagtaaatca agttcgtcag gtttaatctt tatttttttt
     6601 gaaaaattaa taaatttttt ttcaatttca atatattttt ttttagttaa tgtttcagga
     6661 attttttcta ttattctaaa attttttaaa ttttttaaaa tatgcctgtc aagaattgca
     6721 atattatctc caagacctat atttcttaaa aaatgacttg cttccttcat accaaatcct
     6781 tttatatttt taacaagaat ttctcttaag agatgtatat ctttacaggt ctttattaaa
     6841 tcttttaaat tactttcaaa aaattttttt cttgccagaa taatatattt tgccttcttt
     6901 ttattaaatc tcactttttt taatttttct gatatttcac tttcatgccc ataaaaaagt
     6961 aaattctctt ttttcaaagt ttctatcgcc tgccagcaaa tttctgcttt tgattgagga
     7021 gttaaaatac aaaaggaaag ttctgcaaaa atatcttcaa tatttttatt tagattatct
     7081 ttaaattctt ttattctttt ttttatttct ggtttgattt ttttgtacag tttttttaaa
     7141 tcttcttttg attccatttt tcataaaata aaaatgtctc tggcaacacc tactctccca
     7201 cctcctctca gaggcagtac catcggcgtc gggaggctta acgaccgtgt tcgggatggg
     7261 aacgggtgtt gccctcccgc catggttgcc agagacacta taattatacc caaatttttt
     7321 ttatttgtca ataacaattt taaaaaattc aggaacgagg atggtatttc ttgtatatct
     7381 cttttatttt ttttctattt aaatgtgtat atatttctgt tgttgcaatt gatttatgcc
     7441 cgagtaattc ctgaacaatt cttaaatcaa gtcctctttc aagtaaatgt gttgcaaaag
     7501 tatgtcttaa agtatgtggt ttaacatttt tttcaattcc agcatatttt gcatattttt
     7561 taactatctt ccatacactc tgtcttgaaa tttttttact ttggttatta agaaataaat
     7621 attcattgtc aacagttttt ctttgattta aatattcttt aattatttct gttgtaatat
     7681 tatttaaaag ggcgattctt tctctatttc ctttgcctct tacttttaca aaaccacttt
     7741 ctaaatttaa atcgcttatt tttaagttgc ataattcaga aattctcaaa ccagaactat
     7801 aaaaaagttc aagaattgct aaattcctca aatgttttct tgaaatcttg ttaacattta
     7861 ataatttttt aacttcttct tccgtcaaaa catcgggtaa ttttttttct atcttaggac
     7921 tttcaatact aatttcagga ttttttatat atcctcttgc tacaagaaat ttgtaaaaat
     7981 ttctcactcc tgatattatt ctgacaatac ttgttgatga gtagtttttt ctttttaaat
     8041 atattataaa ggaagtaaaa atttcataat tgagtttatt gatgttaatt tttttttctt
     8101 caataaatgc tttgaatttg taaaggtcat ttttatatgc actgactgta tttaaagaaa
     8161 gatttttttc aataagtata tgattgataa acatttctat catttcttca aatgtcattt
     8221 attcatcctt tcttttacaa gtttttcaaa ttcttcctct gttataattt ttatcccgag
     8281 ttgttgggct ttctgtaatt ttgaaccggg ttcactaccg catataagat aatcagtttt
     8341 ttttgaaaca gaatcggtta cttttcctcc aagtttttta atataatcct gtatttcatt
     8401 tcttgaataa ttttttaatg ttccagtaat tacaaatgtc aaacctgaaa gaatatcttt
     8461 tttttcttct gttttttctt tttccatttt tacaccggct tttctcagtt tttcaataac
     8521 tttcaagttt tcttcattgg aaaaaaaatc aacaatactt tctgccatta taggacctat
     8581 ttcggaaata gaagaaagtt tttcaattgt tgcattttta agttcatcaa tagaattaaa
     8641 ttcatcggat aaaatttcac ttgcatgaac tccaatatgt cttattccaa gggcatatat
     8701 aagatttgaa aaaggtcttg tcttactttt atcaattgct tccagcagat ttttaactga
     8761 tttatcaccc attctttcaa gttttattaa gtcatcatac tttagataat aaatatctcc
     8821 ataatctgaa agtaatcctt tgtcaacaag aatattaacc catttttctc caagaccttc
     8881 tatatccatt gcatctcttg atgtaaaatg aataattctt tctttgacct gagctggaca
     8941 tctgatattt ggacatctta ttgcaacttc ttgtatatct tttacaaccc tactcccaca
     9001 aacaggacag gtttctggta cagggatatc tttttctttt cccgttcttg cttccggaat
     9061 aacctttaca acctttggta ttatttctcc acttttctca acaaaaactc tatcgcctat
     9121 ttttacacca agccttttaa tttcgtcaaa attatgtaat gttgctcttg aaacagtagt
     9181 tccacttaca aaaacgggtt caagtattgc aacaggtgtt aatgtccctg ttctaccaac
     9241 ctgaacaata acatctttaa caattgttga aacctgttct gctggaaatt tataagcaac
     9301 agcccatctc ggactttttg ttgtagtccc caatgtcctc tgtaaatcaa gagaattaac
     9361 tttaacaacc attccatcta tgttatatgg tagagtaaat cttttctcac gccattcatt
     9421 acagtactct ataacttttt ctatattttt acagcatttt ctataaggac taacaggaaa
     9481 accaatttct ttgagaaaat tcaaaacttc ccagtgtgta ttcggtccat tatttaaaaa
     9541 accctgatat ataaaaattt gaagattcct ctgagcaaca agttttggat caagaagttt
     9601 caaagaacct gcagtagcat ttcttggatt ggcaaataaa acttcaccct tttcttttct
     9661 ttcacgattt aatttctcaa aatcatcctt cctcatataa acctctcctc taacttcaat
     9721 agattcctta tatttaattt ttaatggaag tgttttaaca gtttttaaat tttcagttat
     9781 atcatcacct ctaaatccat cacctcttgt aactccaaga ataaactctc catcttcata
     9841 atgtagagaa accgcaaccc catcatattt aagttcaaca acataatcta cctctttgac
     9901 atctgccatt ctctttattt tcgcatcaaa ctcaataagt tcttcttctg aatatgtatt
     9961 atccatactt aacattggaa ttttatgttc tattgttttg aattctctta acggttgacc
    10021 tcccactttt tgtgtgggtg aatcaggtaa tttatattca ggatattttt tttcaagttc
    10081 aaccagttct ttatataatt tatcgtattc atagtcactt ataacaggat tattttccac
    10141 ataatacata tagttgtaat aatttattaa tttcacaagt tcttctattc tttctttggg
    10201 agttttagcc ataccagcct cctttttctt ctatttataa agatgttgca atttttttaa
    10261 aatttcaagt ttttcttttc cttcaatttt tgccttatca attatatttt tcaaataatt
    10321 aattgttata tcataatttt tcctatccag tgggtaagga tgcccatcct tacctccaaa
    10381 agcaaaagaa aattttgcag ggtcattaaa tgaaacagaa ttaccataaa taacttctga
    10441 tattaaacat agtgctctta aagtttttat tcctatattt tctgtaagaa caatatcagg
    10501 aaaggattta atatcttttt cttttaattt acaaaaattt ctgtataatc tttttgtatc
    10561 aaagttatat tctacatgat gatgtgaagg aagtataagt tttttccctc tatcaaaaat
    10621 tgaagacgat tttttaaaaa taacctcaaa ttctttaatc aaactatcag gactttctga
    10681 tttcaaaaaa tcaactaaat tattttgaac ttccttactt cttttatcaa tcagatttaa
    10741 aacataactt tcttttttta cagatataat tcctgaatga ggttctataa ccagtttttt
    10801 atcttctgaa cttgtgattt tattgaacca gtgatacctc cttgcataat tatttttcaa
    10861 attcattccc tgttgtataa ctgcccagtt ccctttttta tcaacaaaaa tattatggtg
    10921 ataaagttga tacccatcct gaatacaatt attatcaatt ttacttgtaa gttttgaaat
    10981 aaaaataatt tcatccttcc tatcaataaa tctgttgttt cttatttctt caggtgttaa
    11041 aagcccattt ttacccttcc ctccaaaaat aaaaattcca tattccgggt tattattaaa
    11101 aacatttttt aaagcatgac atactgtcgt agtaacacct gacgaatgcc agtcaaaacc
    11161 agagatacaa ccaagtacct ggaaataata agggtcggat aaacgtttca aaaattcttc
    11221 tgtaccgaat tccattatta tcaacctacc aatctcaaaa agtattttct tctgtctttc
    11281 aaaaagatat tttggtgctg aaccgaaatg taaaggtaac tctgtaatat ttctttccat
    11341 attttaattt tagcaaaatt tttattaaaa tctctatttt cttaattata gtttaaaacc
    11401 agtaaaaaaa aatgtaaaat taaaaaatgg aaaaattgtt aagtatatac aatgaagttt
    11461 acaaaattat aaattcggta tcagagaata aaaatgaaat aacagagata aatcttttta
    11521 atgaaaaaag aaaagcagga attttgttcc ttcctgattt ttgttcttct gtattaatta
    11581 ttttacccgg agcaggatat aatgaaaaat ttcaaaaatc agtttttaaa aattttaaat
    11641 ttttactgaa aaaaaatata gggatatgta ttttcaatct aaataatggg aatttggata
    11701 aagaggatgt tatttttttt aattacttta aagaaaagat aggagaaatc agaggaataa
    11761 ttgagtatat aaaaaatgag ttaaaaataa aaaatgtaaa tcttctcggt ataagttttg
    11821 gtgggatact tggattcatt gttgcagcac tggaaaaaga aataaaaaaa tctattttta
    11881 tactttcagg agttaattta gaatttatta catggaaaag tttacttcgt ttcagaataa
    11941 aaaaggattg taaaagagtt gtgtgtaaaa gaatgcataa ggtatacaga aatcttttaa
    12001 aaaacaatct gtataatgaa attttaaatc ttccaagaaa atgttttctt tatgacccct
    12061 ttacatatct gaccaatctg atagatagag aaatattaat gataaatagt atttttgata
    12121 tgataattcc tttttactgt gtacttgagg taaaaaaacg attaaaaaat gtaaagacgc
    12181 tttggtatcc atcaactcat ctaacattaa aattttttat cccttttatt agaaaaaata
    12241 tactgaattt cttaaaaaat gaaaatatat ccgggaattg atttgataga gatagaaaga
    12301 tttgaaaaag cataccaaaa atatggagaa aaatttctta aaaaaatttt ttataatgat
    12361 gaacttttat atcttgaaaa aaatataatc aaaatgtgta tatctttttc ctttaaagaa
    12421 tccatatgga agtcattacc ggaaaaaata cagaaaaaac tactttttaa agatataaaa
    12481 ataggatggg acaataacaa tccattttta ctgaaaaaaa tagaaaatta tgagatactt
    12541 ctttcttact caatcactga aaaatatata attacaactg ttttattgat tgagatttga
    12601 atttaaaatt atctgatacg tatcaaaagc aatccttaat tcttcatttg ttggaatgac
    12661 aaaaacctta attaaagaat tatctttact tatttcccct tcttttccaa ataccttctc
    12721 atttttctca tcatctataa taattccaaa aaactccata ttctccaatg attttttcct
    12781 gacaagtggg acattttctc caatacctcc agtaaaaaca atagcgtcaa ttttaccaag
    12841 gacagccata taacttccaa tgtatttttt caatctataa cagaacatat caattgcaag
    12901 tgtaactttt tcatttttat tgtaaagggg taataaattc ctgaaatcat tagaaatctc
    12961 tgaaattcca agtaatccac tttttttgtt taatatttca ctaacttctg aaatttttaa
    13021 atttaatcct tctaaaagat aaagaataac tccggcatct atatctccac atcttgtacc
    13081 cattataaga ccctcaagtg gcgtaagtcc catactggta tcaaaagacc tcccattttt
    13141 cactgctgtt acactgcatc cattgccaag atgacaggta atcacattta tatcgtattt
    13201 ccccttaccc ataatttcag atgttcttct tgcaacatat ctgtgagaaa ttccatgaaa
    13261 tccatatttt cttattttat gtttttcata aaaactataa ggaattgcat aaagatttgc
    13321 aatttttggt attgtggtat gaaaagctgt atcaaaagca gcaacctgaa tactctcggg
    13381 aaaaatttct atacatccct ttattcctgc taaattatgt ggattgtgta gaggtgcaag
    13441 aaaaatataa tcttcaattt tttttattac attttcatca acttcaacac ttttttcaaa
    13501 tccctctcct ccatgaacaa ccctgtgtcc aattcctttt atttctctaa tatcttctat
    13561 ttcgttctca agcaatgttt ttgcaataag ttcaatccct ttttgatgtg tttctatttt
    13621 taattgcttc tctattttta tctttccact gtatttaaaa atagaaattt cttcaccaat
    13681 tttttccaca cttccataag aaataagatt ttcttcaggc atctcaaaca atttaaactt
    13741 tatagaagaa ctcccacaat ttataacaag aattttcatt attttttaag atattcttta
    13801 ataaaatcaa ctgctttagc aactgtcttt acttcaagag ctttctcttc atccatttct
    13861 ataccaaatt cttcttcaaa agcagcaaca agttctaaac tctgaacact ctccgctcca
    13921 aggtcttcta caaatctatc ctcaagtctt attttatctg tatcaacccc aaggacttca
    13981 gaaataactt ttttcaccct ttcaattatt tcaacatcat tcattttccc tcctattttg
    14041 aaatttttct acttttttca atatgtattt ttctctctgc ctctgtcatt tcgtaaactt
    14101 ttcttatcac tgtttcttca gaagataaaa gaatcctgtt tctcctttcc attgcaattt
    14161 tagctgtctt taaagaataa tctactccat atcccttcat aaatttcccg ctcacgaacc
    14221 atacaaatga aggaaaatat tttggaagaa tattatctgc tgttatatta cacattattc
    14281 caactattgt tcctgtatta aaaaatgtac ctatgcttgt tttggtatga tctccaataa
    14341 aagaaccaac ttttaattct cctgtatctg tgggctttcc atttaaatat acactcacat
    14401 ttgaataatc attttttaaa tcactgttag tagttaaagc accaagattt acaaactctc
    14461 ctacataact gtgtccaata aaaccatcat ggtacttatt agaataagaa tgaaaaatac
    14521 tttcttcaac ttctccccca attctacaaa caggaccaaa actatttccc tctcttattt
    14581 tcgcaccggg cataatctgt gttttctctc ctatataaga aggaccaata attactgaaa
    14641 atgggaaaat ttttactcct tcatctatat atacaggtcc ttcacttgca tcaataacta
    14701 caaaaggata aacttctgca gtttgagcaa caaaaacatc tttttctgaa ccataaacac
    14761 atgctttttc agaaaatttt cctgaaattc ctcttttatt tattttatta aattcaaatt
    14821 ttatcaattt agaattatga tgaacaagat tccatggaaa tctaatgata gtaaagtcag
    14881 tgtcctcttt ttttacgtta cttatcaaaa ttttcaaaaa atcatttata tttccattcc
    14941 agtacttatt cacactctct ttttttagta aaccataaac aatttctcca ttacttaaat
    15001 atactctttc ctcttttaaa ttcaattcga ctctcctatc aattaaccac ctcccattaa
    15061 caatcagaac atcatctttc aaaaaatctt cttcatttac tttataatta aatttttcct
    15121 tacagagttc ttttaaataa tctctaaccc ataaaattac ttctccttca ggtaaaatag
    15181 aaataatttt ttcaaggata gaattaaaac cacatcttaa ttcaaaaact gcttttaaat
    15241 atgaaattgg tgtgagatta atccaatttt catcctcata aattaatatt ttcattttac
    15301 ctcctgtctt ttaactcatt aaacaggtaa taacagttgt aacaattata tccttcactt
    15361 ttgctcctct tgaaaggtca ctgactggtt ttgaaaaacc ctgtaaaatg ggaccatatg
    15421 cctgagcatt tcccagatac tgggtaattt tataagcgat attaccagaa tttaaatctg
    15481 gaaaaattaa aacatttgct ttacccgcta caggtgaaaa ctcttttacc tttctcttag
    15541 ccacttcctc gtttatggct gaatcaacct gcaattcgcc gtcaatatca aattctctat
    15601 ttttttcttt tgctatcttc actgcttttt gaactttctc caccaatgga tgagaagcag
    15661 aaccttttgt tgaaaaggaa agaaaagcaa ttttaggatt ttcgcccatc aattttttat
    15721 aatttaatcc agttgttatt cctatttctg caagttgatt ttcatcagga gagatgttaa
    15781 ctgcacaatc tgcaaaaaaa taaatttttc catcaggtaa aaccattata aagaaacttg
    15841 aagttgtttt tatcccctca agatatccaa ctgttaatgc tgctgattgt ataacatttg
    15901 cagtggtatt agtagcaccg gcaatcatag aatcacaatt ccctgtatcc acaagtaaac
    15961 cgccaaatat aagtggtctt ctcactattt tttccgccac atttttattt atattttttc
    16021 ttttttcaca atatctatct acaagaatcc caaataattc tgaattttca gggtcaatta
    16081 tttctatttc tgaaagattt gtaatcccaa ggttttcacc tgtttgaaaa attttctctt
    16141 tattacccag aagaacagga tttactattc tttccttttt caattcagaa gcagataata
    16201 aaattctttc atcttcccct tcagggaaaa caactttttt attcaaacct ttaactcttt
    16261 caataaattc ctgtatttta tccatttctt tttctatttt aaattatcag aataacttta
    16321 tccctatttt tctttcggct tcatatgcaa atctataata ttcttttctg cttaatttac
    16381 ttgctgcttc ttcatccaga ataactgtaa ctttaggatg taattgtaat acagaagccg
    16441 taatctgaga agttattggt ccttcaattg cttttgcaac aacttctgcc tttttaattc
    16501 catttgccaa aaatattatt tcttttgctt caagaatagt tcccactccc attgttaggg
    16561 caaatcttgg aacatcttca actttttcaa aaaatctgga gttatctttt attgtctgct
    16621 catcaagagc aaccaatctt gttcttgaat aaatagaaga acctggttca ttaaaaccaa
    16681 tatgtccatc ccctcctatt ccgagaacct gtaagtcaat gcctcctgct tttttaatca
    16741 tctcttcata acttttacag tattcctcta ttttatcgat agaaattttt ccatcaggta
    16801 catgcgtatt ttcttttttt atatttatac cattgaataa attaacatcc ataaagtatc
    16861 tataactctg aggatgttct ggagaaagtc cataatactc atcaagattg aaggtaataa
    16921 cattagaaaa atcaagattt tcttctttat gtatccttat aagttcttga tacagtccaa
    16981 taggagtact acctgtagca agtcccaaaa cacagtttgg ttttttcttt ataaggtctt
    17041 ttactatttt tgctgcttcc ttactcattt cttcataact cttcgttatt atcactctca
    17101 tctttcctcc tttttttatc tttaaaactt tcacctaatt gtttcatacc aagtgaaatc
    17161 tttttatttt ccgggtctat ttcaagtatc ataagttcaa ctttttgtcc ttttcttaaa
    17221 acttcagagg gatgcttaat tggtgtatct gaaatatttg aaatgtgtaa aagtccatct
    17281 ataccctttt ctatttcaac aaaagcacca aaatctgtta tgtgataaac cttacctgta
    17341 acaatagttc caacaggata ctttttttca atttctggcc aaggatttgg ttcaagttgt
    17401 cttaagctga aagagatttt ctgttcttct tttttaatat ctataacttt tacttttaat
    17461 gtatctcctg tagccacaac ctctgacgga tgttttattc ttcctgtcca tgaaagttca
    17521 gaaatatgta agagtccttc aacgccttct tcaagtttaa taaatacacc gtactcagta
    17581 atattaacaa ctttaccttc tacaattgaa ccaacaggat atttttcctc tactttatcc
    17641 catggatttt gagttttttg ctttaatcca aaagaaacaa cctgtttttc aaaatttata
    17701 tcaagaacaa caacttccac cttttcacca atacttagaa gttgagatgg atgactaatt
    17761 cttccccaac tcatatcgtt tatatgaaga agtccaacga cttcatgctc tatttctata
    17821 aaagcaccat aatcaactat attttttaca acgcctttaa ccagttgatt tttacttaaa
    17881 ctttttaaat actctatttt ttgtctttct ttttcttctt ccagatactg tttccttgac
    17941 acaacaacat tttttcttaa agaatcaaat ttcaaaattt taaaaaatgt tttcatgcca
    18001 acatattcat ttggattttt aataggaact atatctgtct gagaagaagg taaaaatgct
    18061 gttacaccaa tatctactct atacccaccc ttaacccttt taaatataac gccctcaaca
    18121 ggaacattat ttttaaattt ttcttccagt tgttcccagt acaacataat atctgctttt
    18181 tccttagata atggaataag tccgtttgaa tcagggtcaa gagattcaat aaccacataa
    18241 acttcttcgc ctttctttaa ttcatttccc ctacctttaa actcttcttt tgaagttaca
    18301 ccctctgatt taaaaccaat atcaataata acttcttcat ttaaaatatc caccactctt
    18361 cccttaatca atgaacctgg tttaaaactt gctatttttt cttctaaaac tttattaaca
    18421 ttttctttta ccatttatgg tacctccttg taccctattt ttttaaaata ttcaattact
    18481 tttcttataa aaaaattagg cgttgaagtt cctgaaacaa ttcctatatt tttaaaatca
    18541 ctgaatttta ttttacttaa atctgcgaat ttttcaataa aaaaagtatt cttacattct
    18601 tttttacata ttttaaaaag ttttttagta ttagaacttt cttttccccc aagaataaaa
    18661 actgcatcag atgattttgc tatttcaaca ccttcttttt ctctctcttc agtaatttta
    18721 cagattgtat ttaaaacaag tatcttattt gattcaacca ttttaacaat atcttttaca
    18781 atctcaaagt aataattaaa atttaaagtt gtttgactta ctacagcgac ttttttccct
    18841 ctaaaatatt tatattttaa caaatcttct ttactttcta ttacttttat atttcttccg
    18901 tatcctttta ttcccaaaac ttctggatga cttttattcc caacaattaa aatattataa
    18961 cctgtattgc tcaaattttc aactaaataa tgaatttttt ttacaaatgg gcaggtcata
    19021 tcataaattt ccagatttga tttttttaat ttttctattt cttttttagc aagtccgtga
    19081 gaccttatta aaaaatattt tcctttaatt tcgcttacag aattgaaaac ttttaaacct
    19141 tttaacttaa gattctccat taccagtggg ttgtgaacaa tatctccaac cgtagaaagt
    19201 gatttcaatt tttcaagcaa tttttctgtt gtctcaacag cccttttaac accgaaacaa
    19261 aatccgatat tttttgcaac aattagtctt ttcatttcag tgttttaatt atttctatta
    19321 ctttttcact aatttcctca taactttctt tttcaaaata aacaggatta ccaactatta
    19381 cttctatttt ttttagtctt ataaatttac tgtttctcgg aagtgcttta tctgttcctt
    19441 ttaatcttac aggaacaaca ggaacattac ttttaattgc aaggaaacca acccctggtt
    19501 tcccttcttt tatcaatcca tctttacttc ttgttccttc agggaaaata acaagtcctt
    19561 ctcctttctt taaaattttt attgcttttt ttaaagttaa aggcattgaa ctttctctat
    19621 caagaggaat tgctcctaaa attctaacta tagaagaaag aacttttatc tcaaaaagag
    19681 aactttttgc gaaataataa agttttctcg gacatacgaa tcctattgta ggagggtcaa
    19741 gataacttaa atgatttgaa gcgacaataa atcctccttt tttaggaaaa ttctctttcc
    19801 ctctgatttc aattaagaaa aaaacattat aaacgaaaaa tcctataagt tttgcaagaa
    19861 gatataaaaa acttctcatt taattaattt tttaatattt tcaaatcttt tacttttttt
    19921 taaaaattca aggtctttat ctttttctat ataactcttt ctattaaatc caagagaaat
    19981 agctattttt aaattcataa ccgctttctc catgtctccg agtaatgaat aatcacatgc
    20041 taaattataa taggaaatcg ggtctaaatt taataattct gcaatttttt tatcaatttt
    20101 taagaccttt ttataatctc tctggtaaat gtatatatgg gacaattgaa aaagaacttc
    20161 caaaaaatca ggatttatct ttattaattt ttcataaaat ttctttttct caaaaatttt
    20221 ttctttcatt tttttcattt tattaaggta tacaaaatac aggtaaataa aaaaagaata
    20281 agaaatataa aaaatgtaat tttatatttt tttccaacag gtagtttttt agtaaacata
    20341 tatccaacca gtgcataaac tgaaaaccca agaaaaaatc ctatcaaaat accgaataat
    20401 tttatcattt ttccagtata tctttaagca atgaaatccc aaccactttt gacttcaaaa
    20461 ctttgatatt ttttctattt ggaactacac catctctttt taccatcatt aagtactcct
    20521 gtttactcaa agatgtttct gaaatatcaa aatcatcagc atcttctaaa taaaaataat
    20581 cattatcttc atctatcaat gtacctatat atatccatgt ggttgaagta tctattacta
    20641 tttttttatt tctataatct ttcatctttc aagaattata gtacagggtc catcattgtg
    20701 tatctcaaca gtcatttttt ttccaaattc tccttcaaca atttttacat ttgacttttt
    20761 tattttttca ataaacttat tataaagttt ttcagcaatt tctcctttct ctgccattgt
    20821 aaaatctggt ctattacctc ctgtacaatc cccatataaa gtaaactgtg aaacaatcat
    20881 taaatctccg catatatcag aaacagaata gttcatcttt ccattttcat cttcaaaaat
    20941 tcttaaagat aaaatttttt tgataacata atcaatttta tcctctgtat cccctttacc
    21001 tattccaagt aaaataacca atcctttttc tatttcccct ttttcttttt cctctacaaa
    21061 caattttgcc ttgctgactc tttgtataac caatctcata gtatttttta taatatacta
    21121 atttgacaaa aaggacaaat taaatataat tttaacatta tggaaataaa attttttaaa
    21181 atagtcggct ctggtaatga ctttgtaatt atagacaaca gaaaaaattt aataaaaaat
    21241 agaaactctc tcgccctaaa attatgcgat agaaaatttg gaattggagc agatggattg
    21301 cttttacttg aaaattctga aaaggctgat tttaagatga gaatcttcaa ccctgatgga
    21361 agcgaggcag aaatgtgtgg aaatggttta aggtgtattt taagatttat atctgagaat
    21421 tcaatttcta aaaagaaaca tttaaaaata gaaacaaaag caggacttct tgatggtata
    21481 attaaaggaa aaactatgaa agtcaggatg aatataatag gtgaaccaaa attaaatgta
    21541 gaaataccag ttgataaatc taaaataatt ggaaatttta taaatacagg tgttccacat
    21601 actgtaatat ttgttgataa tgttgaaaaa gtggatttag aaaaaatagg acctcaaata
    21661 agatataata aaatctttgg aaaaagtggc acaaatgtta actgggttga aattataaaa
    21721 gaaggaacaa ttaaaataag aacatatgaa agaggtgttg aaggtgagac cctttcatgt
    21781 ggaacaggtt ctgttgcttc agcaattata acttctatta taaaaaaatt taaatcacct
    21841 gtaacagtta ttacaaaaag taaagaattg ttaaaagttt attttgatga agattttaat
    21901 aaggtttatc ttgaaggaaa aacattttac gcttttaaag gtgtctgggt tgaaagttga
    21961 atattgaaat attgattctt ttttctttcg ttttagggat tgttttaaat gacttcttgc
    22021 ctttttatta tattttttca ctcttaccat tattttttat ttcagaaatc aacagaaaat
    22081 ttatcataat ttttttatta tttttccttt ccggatatta cataaccgaa aactataaaa
    22141 gaaaattgat tcctgaaaaa atagatttct cacagaattc tgtgattgaa ggagaaataa
    22201 tcaatatcaa agaaaaaaaa tttgaaagag aaatagtatt aaatataaaa aagatatata
    22261 aagacaacga aaataaaata tggaaaggaa aagttcttct taaaacgaga aataatgata
    22321 aattgctgcc cggagaaaat ttaagaataa aagaattttc tataagtaaa atttatccac
    22381 ctaaaaatcc atcggagttt aactataaaa aatttatgga aagacaggga attttttatt
    22441 taatcaaaag tgacagtatt gaaaaaacag aatctagaaa aaatttaaga ttattgaatt
    22501 cttatataag agaaaaaatt gaaaaaagga ttgaaaatta tatgaaattt aatccagatg
    22561 gatacgaaat tgtaaaactt attactattg gaagcgatga cccccctgat tttctaaaag
    22621 aaattggaat aaaaagtgga atatatcatc ttttcgtaat ttcgggaatt cacattatct
    22681 ttttaatttt attttttaaa attcttttta ttccttttca aaaaataaat aatactcatc
    22741 caaagttttt ccctttttta cttcttttta ttctatggtt ttacgacttt ctctgtggtt
    22801 tcaagatacc tgtaacaaga gcagttttaa tggtttcttt ttatcttata tttgaaattt
    22861 ttgagagaaa gatagaacca ataaaatcta ttattcttgc aggtttttta ttacttttta
    22921 taaatcctta taatatatat tcaatttctt ttctgctgtc ctttctttca acttttggta
    22981 ttttaataat tccaggaaaa ataaaattaa aaagaaactt tataactaat tcttttattt
    23041 ctactttttc agcacaatta tttattttac ctgttctttt ctataacttt ggtttctttt
    23101 atccgtttgg aatattaaat aatctattat ttactcctct tgttggtttt ttgacaataa
    23161 actcttttat ttcaatcttt ttaccttttt tattttttat tctgaacttt gcaacaaata
    23221 tttttttaaa acttcttatc tttatctcta atttttcttt aaaagttaat gtctatttcc
    23281 cattattttt tatacttatt tactattttt cccttttcat tatttttgta cctctgaaaa
    23341 aaagtttaag atttatcact tcatttttaa taattcttct tttagttttt tttcaggtta
    23401 atcttaaaga aaataaaaag gaggaggtta tttttttctc tttaaaaaat ccttttattt
    23461 tgattaacaa agaaagcaag ggtattttaa ttacctcaaa caaaattgaa aatcctgttt
    23521 tttacaaaag tactctttat aaattactga aagagaaaaa aattaaaatt gaaaaagcta
    23581 ttataaccgg aaaaaatttc tcagaaaatc tcatttttat ttcaaaatac tgcaaaaata
    23641 tatatatttc tgacagtatt cttaaaccct gttttttcaa ttatgaaaat ataaaaagtt
    23701 ttcataatga tgacaaaatt attacagata actttctttt ttcttttaaa aaaggcaacc
    23761 ttttggtaaa atatgacaac tttaaatttt taataatcct tgatgaagac cttgaaaagt
    23821 caataacaga ggataaatat tttcttatat atctcgtggc atataaaaaa aataataaaa
    23881 acgatgaaat aatcaacagt ctacaatctc cttttttaat tcttcagaaa gaaacaaaaa
    23941 aatttgaaaa tttaaaaagt ttatgtcaaa attatcattt aaataagtca tctattattt
    24001 taaatttaaa aacaggaatg attaattatt ggaggacaaa tgatattaga aaatattgaa
    24061 aaaattaaaa agggtgaaga acaaagtgaa aaaattataa aagatgctga aaaaaaagtg
    24121 aaagagatac ttgagacatt agaagcggaa gttgaaaaaa tcaaaatgga taaagaaaaa
    24181 ataatgaaag aaaaactgga aaagtataaa cagaaaaaag attttgaaaa aagagaaaaa
    24241 attgaatttt tagaaaaaca aaaggaaaaa attgaaatag atttacaaca gaaaaaaagt
    24301 aagacagatg aagtagcaaa caaaatttgg gaaaaaataa agaaggaaat tatgcagtaa
    24361 caactctatc ccttcctaac tcttttgctt tatataatgc agtatccgct tttttaatca
    24421 attctgtagt tgtcattcca tcttccggga aagaagcaat accaaaactt atagttaaat
    24481 ctttatttgg ttgagattct tcatttaaaa atttaaattc ccttatagtt tttctcagtt
    24541 tttcacaaat tttcacggta ttttcttttg ttgtatctgg aaaaataata ataaactctt
    24601 ctccaccata tctacaaaca atatccgaac ccttaacccc cttttttaat atatctgcta
    24661 tagttttcaa aacataatca ccagcgagat gtccatttgt atcattataa aatttaaaat
    24721 aatctacatc acacatagca atagaaacag gtttataatt tacccgactt tttgttagaa
    24781 tttctgaaaa aagtttttca aaatgtcttc tattatacag ttgtgttaaa ctgtccataa
    24841 ttgagagaat ttttgtttta ttgaaaagtt ctgtattctc aatcatcaat gaaataggaa
    24901 cagataccat tcttaaaaga ttcaaatctc ttaaagtaaa tcttttttcc ctttttgaaa
    24961 aaataccgag tattccaatc acagttttct tctgatataa accgcaaact ataaacgatt
    25021 taatcccatt ttcagcaagc actccatatt gtgaaaaaga agatatatta ttaacaagaa
    25081 ctggtcttcc ttttatagct aattcttcta tcaattcttg agaaatttcg atatgttctc
    25141 ctttaacact cattttgtat ctatcaccga aaagagtaat aacaataatt tcttctttaa
    25201 aaatattttt aatggtatca agaatttttc caataacatc atctatagtt acatcctctc
    25261 caaaagtttc tataatttca gtgatggtgg atagaaaagt agattctatt ttatagtcat
    25321 taattatcaa atcttttctt tcattctctc tctgtaaaaa ttcaatttta tttaaaatct
    25381 gaaaagcaaa aataatatcc agaacaatta aaatagaaat aataatgtta aaagatataa
    25441 gataaaattc cggaataaag gtttttcctt gtagatatat ccagttgatg accaaaagaa
    25501 ataaaattac aaataaaaaa ataaaattcc ttaaaaccct cattcaattt accctattcc
    25561 ccctttattt gattaaccag tgaggcaaaa ttttcagggt gattaaaagc aatatctgaa
    25621 agtgtttttc tgttaagatt tataccaagt cgtttaagcc ctgctataaa tttactgtaa
    25681 gaaatcccat ttgctttaca tgctgcagaa attctggtta tccacaatct tctaaaatct
    25741 cttttctttt tccttctgtg catgaaagca taggttaacc ccttttctga aaactccttt
    25801 gctcttttaa aaagttttga ccttccttgt ctataaccct ttgctctttt caaaactttt
    25861 tttcttcttt gtcttgaact gggtgaattt ttagttcttg gcattttttt tctccttttt
    25921 aattatttac ctataaggta ataatctttt taccattttt atatcgcctt tattcttcaa
    25981 aacctgtgac ttcttaattc ttcttttcct ctttgaactc tttttagaaa gtagatgact
    26041 tccacccgcc ccataatgga ttactttacc tgtttttgta attttaaatc tttttgccac
    26101 cgattttttt gttttaagtt ttcccatttt tttctccctt tacatctatt ggaataaatg
    26161 taacacttaa tctaacacct tcaagtttag gtgctttatc tattttccca tattcagaaa
    26221 gttcccttat caacctttcc aaaagagcat aacctctttc tttgtaaaga atttctctac
    26281 ctttataaaa aacacttact ttaactctgt gaccttcttt taaaaaattt tcaatatgtt
    26341 gcttcttaaa cttataatca tgttcacttg tttccggagt aaaccttatc tctttcgtct
    26401 gcaatgtttt ttgttttttc tttatttctt tttctttttt cttctgttca aataaaaatc
    26461 ttttaaaatc caaaattctg catacaggca aatttgaatc tggaactatc tcaactaaat
    26521 ccaaattctt ttcttttgca atgtctaaag cagtttttat atcaagaatt cctaattgat
    26581 ttcctctttc atcaataact cttacctttg gacttctaat ttgataattt attctatatc
    26641 ttctttcttt accttcccga gcgatgatat ctccttttta caaattttaa cttatatttt
    26701 tacttttttc aatacctcta taaattttaa ttattttttt aaaaattgca agtttaattt
    26761 taattcaccc atttaaagta aaaagtgtta taatttgtaa aaggatttta aaatgaaaaa
    26821 taaaatcttt gcgtttttat tcataataaa tattttttgt ttttctaaaa ctcagtttat
    26881 aaatagaaca gaatttctgc ttgatacagt atttaatata aaaattgaaa agattgaaaa
    26941 tgcagatatt atactggaaa gtgcttttga gagggtaaaa gaacttgaag ataaattaag
    27001 tatttttaaa gaagaaagcg aagtttccag attaaataaa tataaaaaat ttaaagtttc
    27061 atcagaactt atggaagtaa taaaaaaatc tatttatatt tctgaaatta cagatggtgc
    27121 ttttgatatt acctgtaaaa aattaattga tttatacaga aaaaaaaata aacagaattc
    27181 attaccaaca aaagaagaaa taaataaagt tttgaaagaa actggctggg aaaaaataaa
    27241 gataaaagat aatgaaatat atttatcaaa aaatgctgaa atagaccttg gaggaattgc
    27301 aaaaggtttt atagttgata aagtatttga attccttaaa tctaaagggg taaaaaatgg
    27361 gattataaat gcaggagggg atatctattg ctggggaatg aatcaggaaa acaaaaagtg
    27421 gaaaatagga atagaaaatc catttaaaga aggggaaata atcggaagtt ttgaaattac
    27481 agataaagga gtggcaacaa gtggaaatta taagagatac atgaaaataa agaataagaa
    27541 aataggacat ataataaatc caaaaactgg actacctgtt gaaaattttt gtgtaagtgt
    27601 aactgtaatt tcttctgact gtacaactgc cgatggactt gcaaccgcta tttttgttct
    27661 tggaattgaa aagggattga aaatttttga aaaatataaa aattttgagt gtttaataat
    27721 agacaaagat aaaaaaattt ataaatctca aaattttcct tttaatcctt acattttttc
    27781 cagtttaaat aaataagaat atgtctctct tttccttatt aatttcacat ttttacctgt
    27841 aacaagaact tctggaattc tcaatcttgt attgtaattt gaagacattg aaaaaccata
    27901 tgctcctgca ctcataacta ctaaaaaatc atttctttta atatcctctg gaaattctct
    27961 atctttacca agataatctc ctgtttcaca gataggacca actatatcaa attttatttt
    28021 ctttccattt tttaacttca aaggataaat aagatgataa cttccgtaaa gagaaggtct
    28081 tatgaggtca ttcatcccag catctacaat tataaaattt tttgtatgtt tgtgtttaac
    28141 ataaagaact tttgtaatca aaacacctgc attaccaaca ataaatcttc caggttcaat
    28201 tattaaggtt tttaaattta aatcttttaa tgtttgataa atttcttttc caaattcttc
    28261 aactgactgt attctatctt cttctttata agggatacca aaaccccctc ctatatcaag
    28321 tatttctaaa ttgaactcat tttttttaca gaactctcta actttttcaa ttgccttaat
    28381 ataaggagaa acttctttta tttgagaacc aagatggaaa tgtatccctt taatttctac
    28441 aaaattaaat tttttcttta aaattctttc agaaatatca atatcaattc cgaatttatt
    28501 ttcacttttt gatgttttta tataatggtg cgtatcaaca tcaatatcaa gatttaacct
    28561 taaatttaca acaacctttt ttccatattt cttcccaaat ttctcaatta tatcaagttc
    28621 ttcttcattt tctacgttaa aacagaatat atttttttta actgcaaatt caatttcttc
    28681 ctctttcttt ccaacccctg caaaaacaat atctgacgga gaaaatccag caataagtgc
    28741 tcttcttaat tccccttctg aaacaatatc tgctccaagt ccgtttgcct tcattatttt
    28801 taaaatagaa atattagaat ttgattttac agaaaaacaa atcttaacag gtaaattgga
    28861 aaaagcattt tttattctgt ttatttgatt tttaagaaag gagtggctgt agacataaac
    28921 aggagtaccg aatttttcaa caatttttct accctctact tcttcaatat agaaacttcc
    28981 ttttctaaat ttataataag gttccaagat ttctttaatt ttttcttcca tctctcaatc
    29041 tcctttttaa catttattga agaagtccct cctcctgaaa tttttgaatt taccgaattt
    29101 ttaaaattta aaatttgtaa aatgtctttt tcaaaaattg gagaaaaact tttgaattca
    29161 tttaaagtta aattaaaaaa agttttattt ttctctatgc agtattttac aatattcccc
    29221 actatcctat gtgcttctct gaaaggaata ccttttttta caagatattc aacaatatct
    29281 actgataatg taaaagatga aattttttct atgattattt ctttattaaa tttaatttcg
    29341 gataaaattt ttattaaaat tttgagagtt gcttctactg tttttaatat ttcaaataat
    29401 atctttttat cttcttgaat atctctattg taagaaagag gtaaatcctt gagaagaaca
    29461 agaagtccat taagaagacc agtacaaact gcagtttttc ctctgattaa ctcaaataca
    29521 tcaggattct ttttatgagg cattaaactt gaacctgtac aatacttatc cggtaattta
    29581 acaaaattaa tttcatccat attccatatt attatgtcct gagaaaaaga tgaaagatga
    29641 atcattgtta ggagacaaca aaaggttacc tctactaaaa aatctctatc agaaacagtg
    29701 tctaaactat ttcttgaaat ttcagggaaa ttcagaagtt tagcagtata ttctctatct
    29761 atgggtaaag aggttcctgc acaggcaccc actccaagag gaaaaacatt aatcctttta
    29821 taagtatcca taaatctttc tttatctctc tctaattttt caacataagc aagtaaataa
    29881 tgagaaagta aaatcggttg actttgtttc aaatgagtat acgctgaaat tattgtatca
    29941 aaatattctt cacttttttc aagaaatttt tcctgtaaat ctattatcaa ttttactaaa
    30001 taaataactt catctcttaa aaaaagtttt tcgtcaagaa ctatctggtc atttcttgac
    30061 cttgctgtat gcaacttttc gcctatttta cctataattt ctatcaacct tctttctatt
    30121 gctgtatgaa tatcctcatc acttggtaaa aacttaaatt tattttcttt tatttctttt
    30181 ttaatatttt caagccctct tataattttt tctttttctt cctctgtaat caatccgact
    30241 ttaaaaagca tttttacatg agcgatactg ccttcaatat cgtaaattgc aagtttttta
    30301 tcaacattga ttgattttgt aaattctata atatcaacat ctactttctc tttaaatctt
    30361 ccttcccaca tatatctcat ttttcacctc ttgttttttt attttatcag aagtattata
    30421 ataaatcaaa gaataaaaag gattaaattt atggaaaaag aacttataat cgttgaatct
    30481 ccaacaaaag caaaaacaat tagtgggata cttggtaatg aatatacagt atttgccact
    30541 atgggacata taagggacct tcctgaaaat gaatttggag tggatattga taacaatttt
    30601 tctcctaaat atgttataat acctggaaaa aagaaaattg tggaaaaact gaaaaaactt
    30661 gctcaaaatt cctccaatat ctaccttgca actgatgaag atagagaagg tgaagcaatt
    30721 gcatggcata ctgtacattt aataaataaa aaaattgaag atgtaaaaag agttgctttt
    30781 catgaaattg ttcctgaagc agtaaaagaa gcattttcaa atccaagaca gatatctatt
    30841 gaacttgtaa atgctcaaca gacaagaaga attcttgaca ggatagttgg atataaaata
    30901 agtcccatac ttggtaaaca tcttgaaatg ggattatctg ctggaagagt tcaatcagtt
    30961 gccttgaaac ttattgttga gagggaaaaa gaaattgaaa attttgttcc tgaaatttat
    31021 ttcattataa aatgtgaagt agagaaagac ggtaaaaaaa tagttttcaa tcttatcaaa
    31081 attggtgaaa aatcatttga aaaagaaaag ttaaaagaag aaaaagaagt agatgaaata
    31141 attgaaaagt taaaaaatgg aactcttatt gtgaaggaaa aagaagaaaa aataaaaaaa
    31201 ttaactcctc caccaccttt tataacaagt actttacaac aggaagcaat aaatatttta
    31261 aatttttctt cctcaaaaac catgtttatt gctcagcaat tatatgaagg aattgaaata
    31321 gaaggaaaaa gtataggatt gataacatat atgagaaccg attctccttc tgttgcaaaa
    31381 cctgcacaaa atcaggcatt aaaatttata aaagatacat ttggagaaag ttatttacct
    31441 gagaaaccaa atatttatta ttctaaatca aaagtttctc aagaagcaca tgaatcaata
    31501 agacctactt ctgttttcag aacgcctgat aaaataaaaa aatatttaaa ccctgaccag
    31561 tttaaacttt atgaactaat atggaaaaga tttattgcaa gtcaaatggc caaagcaact
    31621 ataaaaaata ttaaagttac tgcgttaaat gatatttttc tatttgaaac agaaggtaat
    31681 tttattgttt ttgatggttt tttaaaatta tggcctttaa aaattgaaaa agggagccaa
    31741 attataggcg aatttgaaaa ataagaagta ttaaatgttt tagaatataa aaaggaaaaa
    31801 aaacaaactc aacccctttc aaggttcact gaaagttcac ttattaaaac tcttgagaaa
    31861 tatggtattg gaagaccatc tacctatgca ccaaccattt ccactttaat tgcaagaaaa
    31921 tatgtaaaaa aagaaaaaaa attccttgtt ccattaagaa tgggtaaaat tgtttatgaa
    31981 atattaaata aatttttccc tgaaataata gaaataaact ttacagcaaa aatggaagaa
    32041 gaccttgata aaatagcaaa tggagaaaaa aactgggtag aagtattaaa agagttttat
    32101 aataaattta aaaaatctct ggaaaaaact aatgaaatat taaataggga tattttgaac
    32161 gaaattataa tagaggacaa aagatgtcct ctatgtaatg gacaattaac cttaataaaa
    32221 ggtaagtatg ggatttttat tggttgttcc aattatccta attgtaccta tactgaaagg
    32281 ataaaagaaa atgctgataa ttccggcaat agatctaaaa aaaggtgaaa ttgttcgtct
    32341 ctataaagga agatttgata aaatttccta ttatgatgtt gagattgaaa atgttacaaa
    32401 agaatattta aaagcaggtg caaaaaggat tcatattgtt cttctatacg gtgcaaaaac
    32461 aggcaaaata gaaaatgaag aaattgaaaa aattaaaaac ataattgaat taaaaaaaat
    32521 aaataacaga gatgattgtg aacttcaaat tggcggaggg ataagaaaaa gagaacagat
    32581 agaaaaattt ttaaatttag gaataaatta tgtaattata ggaacagcat ttttaattcc
    32641 tattgcttta agtgaaggat acagtattca ggatattaga tttttttatc aaaaaagtgg
    32701 caaaaatctg gaagttgaga aagaaattcc tgaatttgat ttaattgact ggcttgaacc
    32761 agaaattaaa gataaaatta taatatcagt tgattatgtt ggagacgaaa tcgctttaag
    32821 tggatgggaa gtaactttac ctttaaaacc ttcatatgta ataaaaagat ttatagaaaa
    32881 aggattcaaa aggtttctga taacaagtat tgaaagcgat ggaactcttg atggaattga
    32941 ttttaaaaat atcagtagaa taacgaaaga aattaccaga tttaaagaag atataaaaga
    33001 aataatagtt gctggaggaa tatcaaagga agaagatata ataatgttga actctttaca
    33061 atatagacca acaggaatta taataggaaa ggcattgtat caaaaaaaat tagatttaaa
    33121 aaatgttatt gaaaaatatc aaaaaattta gtttattttt aattttattt tttaatctgg
    33181 tttttgcaca atttaaggca aatgttactc ctttattgaa tgaaaattat tcaaagagta
    33241 ttctaaattt tataaaaagg gctgaaaaat ctatttatgt cattatgttt caaacaggat
    33301 attattctga atatgcagaa agtatatcaa atcagatatt gagagaaata gtaaacgcat
    33361 ataaaagaga tgttaaagtg gaagttatac ttgatttgtc tacaatgtct aatgttaaag
    33421 aaaaaaattt tgaaaccgca aaatttcttg cagaaaatgg aattaaggtc tattttgata
    33481 aagcagacaa gacaacccat tctaaacttt taatagttga tgataaatat gttttcatcg
    33541 gaagtcacaa ctggaattat tacgcacttg aaaaaaacaa tgaaacaagt attttaattg
    33601 aatcagaaga aaccgctagg gtattcatta attattttaa tgaagtaaaa aaagaatgta
    33661 gaatatttct tgcaccactt aactaatcct tctctattgt ctgtcctctc gatgaaccag
    33721 ttgaaatttt tgttattttt gtgcctattt tttcctcaat tttttctata tatttcctgg
    33781 cattttttgg taattctttg taacttttaa cattacttat atcttctttc cagccatcca
    33841 tttcttcgta tataacttca caatttgaaa aaagttttgg attaaaagga taggaattta
    33901 taattttttc ttcatattta tatcccactc ctattttaat tttctcaaga cctgttaaaa
    33961 catcaagttt tgttaaaaca atttcatcta tcccgttaac catacaggca aatttaacaa
    34021 gaactgcatc aaaccaacca catcttctcg gtctacctgt agtggctcca aattcattcc
    34081 ctatctttct taattcttct cctatactat cctttatttc tgtaggaaaa ggtcccatcc
    34141 ctactcttgt tgtgtatgcc tttgcaactc ctataatttt ttctattttt ttcggagata
    34201 taccaagccc tatacaagca ccaccagaaa tcggatttga ggcagttaca tatggatagg
    34261 taccaaaatc tatatcaaga aaggttccct gagctccttc tgcaagaatt ttttttcctt
    34321 tttcaatttc ttcattaaga tagattgatg tatttataac aaatttttta atcttttttc
    34381 tatatatctc ataatcctta taaatttttt cagcttttaa aacttcttcc ttataatatt
    34441 ccttaaataa gtaattcttc aattcaagag cgatttttaa tttttccata aatacttcat
    34501 catctataaa atcaacaact cttatcccaa ttcttgcaaa tttatcagta taggtggttc
    34561 caattcctct tcctgttgtt ccaagttgtc ctttgcctct aaacgtatct tcaacccggt
    34621 ctaaaatttt atggtagggt aaagttatat gtgcgttttc agcaataaat aattttccct
    34681 ctacttctat tccattattt tttaaatatt caatttcctc aaataaagaa accgggtcaa
    34741 taacaactcc atttccaata acacaaattt tttcaggata aaggatacct gagggaataa
    34801 gatgaaatac atatttttta ttatttacaa ctactgtatg acctgcattt gctccaccct
    34861 gatatcttgc aattatatca actttctctg caagataatc aattactttt cccttacctt
    34921 catctcccca ttgggttcca acaacaactg ttaccatttt cctcccattt tctttataat
    34981 tgcctttccc atctctattg tccctacaca ttcttcattc gggtttttaa aatcataagt
    35041 aacaaatttc ccttcttcta taacttctgc cagcgccctt tcaattttat cagatgcttc
    35101 tgtttctcct aaataatcca gcattaaaat tccagcaagt atcatggcag agggattaac
    35161 tctattttgt cctttatatt taggagcaga cccatgggtc ggttcaaaaa ttgcaatatc
    35221 ttctccaatg tttgctcccg gtgaaagtcc cagtcctcct acaagtcctg cacacaaatc
    35281 agaaagaata tctccataaa gatttggaca aacaataaca tcataaattt caggcttttg
    35341 gacaagttgc atagccatat tatcaacaat tttatcctca tattctatat cagggtattc
    35401 ctggcttatt ttatttgcca cttcaagaaa aagaccatca gaatatttca ttatatttgc
    35461 tttatgaaca acagtaacct tcttcctatt attttttctt gcatattcaa aaccaaatct
    35521 aacaattttt tcagaatttt ctacagatat cggttttatg cttattcctg tgtttttact
    35581 taacttaaca ttcctttttt tctcaataaa tccaattaac tcataggtat cttctttccc
    35641 tttttcaaat tcaattccag cataaaggtc ttccatattt tctcttataa gaacaatatc
    35701 aacattttca tattttgttc gtgcaccttt ataaatttta aacggcctta aacatacata
    35761 caaattaaaa tactgcctta aaaaaacatt tatacttcta aaaccagtac ccacaggtgt
    35821 cgttatagga ccttttaaac atattttatt ttcttctatt gattttattg tatcttcagg
    35881 tataggtgtt ccaaattttt ctatacattc ttcaccagcc atttttacat cccattcaat
    35941 ttctactcca gtcgcttcaa tacatttctt tgctacttca gtgatttcag gacctatgcc
    36001 atctcctgga ataagagtga ttttatatga catattattc tccaatttta aattttttat
    36061 acttttttaa ataacttatt acaccaccct ctttaataat atctatcaaa aaatcaggaa
    36121 ttttcgttgt tttaatttct atgttttttg ttatattttt tattatccct tcaattggat
    36181 atatttctaa ttcatctcca ttttcaatct tatctgtatc accttcaatt aaaattaacc
    36241 cattatttat accattccga taaaatattc ttgcaaaact tttagcaatt acagcaggaa
    36301 tccctgcatg ttttattact aatggcgcct gttccctact acttccacat ccaaaattaa
    36361 ttcctgcaac aataaaatct ccctttctaa tctttttata gaattctggt tcaatatctt
    36421 ccattagatg tttagcaagt tcatccatat caagtgtttt aaatttatat tttcctgaaa
    36481 ttatataatc agtattaata ttatctccga atttatgact ttttcctttt agaaccatta
    36541 attctccttt ttacactcaa ttatttttac cataaatttt aaaataattc aaattgaaat
    36601 agaattcgaa aaaagataca attattttta aaaaaggagg taacaaatga atatccttgc
    36661 aattggtgca catccagacg accttgaaat tctgtgtgga ggaacacttg caaaatactc
    36721 aaaaaatgga cacaaaatct ttatggctca tttatgtaat ggaaataaag gtggaaaaaa
    36781 catatctcca tccgaacttg ctaaaattag agacgaagaa gcaaaaaaag cagcaagttt
    36841 aataaaagca gaagttttag gaccaattgc ttcagacctt gaaatttaca ataccaaaga
    36901 aatgagggaa aaagtagttg atataatcag atatgcaaaa ccagatatta ttattacaca
    36961 ctcacctgaa gattatatgc cagaccacac aaatacagca caactagtat ttgacgctgc
    37021 gtttacttct acccttccaa attacaaaac tgaatatcca gcacacgaac atattacacc
    37081 tattttttat atggatacac ttgtaggtct caagttccag ccaactcact acgttgacat
    37141 aactgatttc tttgaaataa aaaaacagat gttactatgt catgaaagtc agtataaatg
    37201 gataaaagga catcatcttt cagacccttt aaatatcatt gaaacagtgt caaaatttag
    37261 aggtttacaa tgcggtgttt tatatgctga agccttcaga atggaaaatg tctggggaag
    37321 gataaaacct gctgaactca tataaatttt agaaaaatga aaaaagttct tataacagga
    37381 gggaatggac ttgttggtaa ttatttgaat agaatatttg atggaaaaaa ttattcagta
    37441 ttttctttta acaaggaagt acttgatata acaaatatta aaaaaagttt tgaaattttt
    37501 aaaaatataa aacctgaagt agtgatacat cttgcggcac taacagatgt tgatttttgt
    37561 gaaaaaaatc ctgaaaaagc atttttagtg aattcacaag gaacagaaaa tattgcgaaa
    37621 ttatcatatg actttaactg ttttttgatt tatttaagca cagattttat ttttgatgga
    37681 ttaaaaaatt ctccttactc agaaaaagac ctaccaaatc cgataaatat ttatggaaaa
    37741 agtaaacttc tcggagaaaa atatataatg aataactgta agaaatattt aataataaga
    37801 acttccagaa tttttggtaa agtaggtaaa aattttagca gtaaattaaa agaaattata
    37861 agggaaagga aagaaatatt tttgacaaca gatgtaataa attctccaac atatgcactt
    37921 gacctttcaa attgtatttt taaattgatg gaaatggatt tttgtggtat tataaatgtt
    37981 tgtaatgaag ggtggtgttc tttttttgac ttcggaaaca aaatgaaaga gattttaaaa
    38041 ttggatacag aaatcaaggg aataaatttt gatgattttt ctaaaaaata tggaattatt
    38101 gctaaaagac ctaaattttc agcattatct attgatttac ttaattcttt aaatataaag
    38161 atggattctt gggaaacaag tctaaaaaaa tttctggagg aggaatagga aatgaaaaaa
    38221 gtaaaaattg gttttgtagg atgtggggga tttgcaaata tggttcatta tccctcattg
    38281 agcgaaatag aagatgttga aatttctgcc atatgtgata ttgatagaga aaaacttaaa
    38341 aatacaggag agaaatataa aatagaaaag agatatacag aatacaaaaa atgttagatg
    38401 aaaatgaatt tgatggagtt tatataatta tgcctcccta tcatttatat gatattgtta
    38461 tagatgcttt aaatagaaaa ttgaatgtat ttatagaaaa accaccggga ataactaaat
    38521 ttcaaacaca aaatatggca gacctggcaa ttaaaaacaa ctgtaaaaca atggtgggtt
    38581 tcaatagaag atttattcct ttattaagaa aagtaaagga aattgttgaa aaaaagggac
    38641 ctataattca ggttgtttca accttttata agaatatgta tcccaatttt ctctattaca
    38701 atggagcagt tgatatatta acctgtgatg gtatccatgc agttgatact ttaaggtggt
    38761 tgtgtggaga agtaaaaaaa attaaaagtg ttataaaatc atatttttct aatataccaa
    38821 atgctttcaa tgcaataata gaatttgaaa aaggtgctac tggaattcta ttgacaaact
    38881 ggacagttgg caaaagaatt catacatttg aaattcattc acttgggatt tctgcttttg
    38941 ttgactgtaa tgacagagca ataatattta aagataatag agaagaacct gaaatttttt
    39001 cagcttttga tataaccaaa agtaaacaat ttcatagatt ttatggttat tacaacgaaa
    39061 atagacattt tattgaatgt ataaaaaacg ataaagaacc agaatgtaat ttcaccgatg
    39121 ctgttaaaac aatggaactt gttgaagaaa tttataaaaa caatatttct ttttaaaaag
    39181 gagtcaacta tataaatttt aaaaaatgaa aaaactaact aaacaacctg ttatcaagga
    39241 gcaagtaata aataacctta agcaatctgg tagaagtagc tataatcgcc tgtctataag
    39301 attttccttc attaattttt ttaatatagt aatctctgaa ataaggagtc atttttctaa
    39361 cagattcagc cataataaaa gcaattcttc ttacatgact atttcc
//
LOCUS       DAOSAT010000003        38572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_790_length_38572_cov_6.096866, whole genome shotgun sequence.
ACCESSION   DAOSAT010000003 DAOSAT010000000
VERSION     DAOSAT010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 38572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38572
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_790_length_38572_cov_6.096866"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PLF90_00415"
     CDS             complement(<1..171)
                     /locus_tag="PLF90_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HOK55778.1"
                     /translation="MNEGIFSGYCKIVAISAGGSHTVALKKDVKVVAWGSNLCGQCNV
                     PEGLEDVVAISAG"
     gene            complement(669..3332)
                     /locus_tag="PLF90_00420"
     CDS             complement(669..3332)
                     /locus_tag="PLF90_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK55779.1"
                     /translation="MRIKKISVKNCGPIKEFDEDLASLNVIYGKNEQGKSFLVEFLIK
                     CLFTNTSNWGYLRNIESSRGKVILEGLGGREVEFSLSSRSKKEQKLEDYFEKDPRGLP
                     PSLCRLLVVKGGEFEIVKNESGVDKNLINDILSGKRILNSIKSNISATIQNAEIEERI
                     SINKMGEGKRYYEIKERLEKVNRIIEDVTKQYEAGEIKKIEIEIKELEEKKENLLKAK
                     RYKAYLLSEEINKLKEKIEKLPHDEKISELKNFIEEFNKREERRKSMSNELDKIKKET
                     ENLQSLERKLEKILKAKRYLAYSLANEIKQKERELEKIPEDELSGLEGDISRYNEKNN
                     ELQRKISGLSELQEKTKHYEWLKEAKEYYLKYLSTPVRFTKFIEALPYLSLLSLMIAT
                     VLFLLGQRIPGTIFMLLTSISILWYKIGIKKYLAGIKQKEEVKSISDEFKKYFGFELN
                     NVADIVSILNEQTTDYNKIVYLKDEISRIQENIQNLKVSIQDKFKKLLGTDINEQNWL
                     DMLSELKNKKDDISKNIEDLKKEFNKLDVDETDFVKENPGIKFEKSEFERISKEVERL
                     RELKRQEEEKSNEFQKFNDELKDLEKEIKQAFSEIIGEEVNQHEWKRKIQEIEKKRND
                     TLKNMKEKEGELRGLGVSEGEYERQNPGVEFSPGKIKEIENRINELKKTRENEGEKLL
                     NLKSEICSFTDSDISSDWNLLIEKLYLKKEEIVRELNEVESNIIAGKLVSETIEELLR
                     EENEKIVEVMNSEEVKKLLLTLTKRYNKLSFDDDGNLLVSSDYETFPLKDLSTGAKEQ
                     VILALRMGFAKIVMGEDHAFLILDDAFQHSDYERRPILVESLVDLAKAGWQVIYLTMD
                     DNIKELFDEKAKKLKNRYKKIELQTTSYNIA"
     gene            complement(3329..4411)
                     /locus_tag="PLF90_00425"
     CDS             complement(3329..4411)
                     /locus_tag="PLF90_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOK55780.1"
                     /translation="MKIAITSDVHLKNCSESPERYNALENIFSKITHKGISELIIAGD
                     LFDKDYNNYSDFDELCKKYPNVSVSIIPGNHDYKIENRFFAATNIKVITEPQIKEIDG
                     LPILFLPYNPEKSMDEVIVEFSHRNTFPERWILIGHGDYITINKELNPYEPGFYMPLT
                     AMTVNNYNPAKVILGHIHKHSEFGKVVYPGSPCGLDINETGKRRFLIYDTQSLVLEYE
                     TVETDVIYFNETILMYPMDDEISYLKDRFYEIIQKWQLSEDELKKVKLRLFLKGYTKD
                     KNVITQNLVQIVRDKGIEFYDSSGPDTSELKILKETDRERILLLNKVKIKIENIVKSI
                     DKRECVSSTDDIMKKVMELIFRSENK"
     gene            complement(4590..5573)
                     /locus_tag="PLF90_00430"
     CDS             complement(4590..5573)
                     /locus_tag="PLF90_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55781.1"
                     /translation="MAKGRTISATELTEKDKAALEICRKISDGLADLITNTESRKDFV
                     KNYGRIHLSRDAGLGMAGAKLQRDALCTRGRTDKAPYSNRNLRWHPLIVANEIPEYAK
                     EVEYVSIDNKSLVFVINGELWYPKDVHKLEEVYCVPYDKWYAIKDDLLQWTDDDWTNN
                     SCVIPAMEYSTLEYSVETFAILGIAVVSNFYGVNPVNAYNLVKEIFLNSNYNPTREYP
                     NQNEIQEIILCPLCRAPLNKPPANLSLPEREEVFQPPWRKSKREEGEAESLQLFHTFP
                     LKESKILHTPRLVRYGHRWCNVAMADHSVDETVEFMQAVVDAHERKKKNRK"
     gene            complement(5576..7591)
                     /locus_tag="PLF90_00435"
     CDS             complement(5576..7591)
                     /locus_tag="PLF90_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOK55782.1"
                     /translation="MARKSYLVVKPLDLFTPTLLPLGLPEVFKQIYYYLYSNSNIPRA
                     ERLGAEMIRILFCKIYDETHNKNDKEFIIRADENEKEVSNRIKKLFEKVKDSYPDVFD
                     REEKILLDDKSIKYVAQKLQGYVLVESERDVISEAFQAFWGPGLRGEKGQFFTPRNVV
                     KMCVDILNPKPDEKVIDPACGSGGFLVEVLSHLKERGHVCNVYGIDKEIDLVKICKAY
                     MSIVGNGHANIFCADSLYPESWPENMKEKIKDESFDIVLTNPPFGAKIFIEDKNILKN
                     YKLGHKWIKDKSNNWKISNFVSNQVPQILFIERCLQLLKPNGRMAIVLPDGVFGNPSY
                     RYVWEFILKNAKILAIVSLPPETFLPSTHTKTSVLFLEKTQNIKKDYDIFMAIANKVG
                     HDKNGKVVFKMDMKGNYVLDNEGNKIIDDDLPLVSSMYKEFIKNVLHLSHLGFTLKLS
                     DVRNFIFIPNYYNPETTQILNKMERTGKYKLTTIKELVEQKWISVKRGHEVGSKYYGN
                     GNIPFVRTSDIVNWEIKIDPIKCVPEEVYEKYKKRQDIRENDILLVTDGTFLIGRTAI
                     VTSLDKKLVIQSHIRRIRCLKPDKLHPYLLLYLLNTDIVQKQIEEKTFVQATISTIGE
                     RLYEVVIPIPTDKSTVEEIISEVSEIIKMKVEARIKMEKLIKTGGRE"
     gene            complement(7594..7947)
                     /locus_tag="PLF90_00440"
     CDS             complement(7594..7947)
                     /locus_tag="PLF90_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55783.1"
                     /translation="MNQMIKILKADEGMIKVTFPYNPVFVEKIKTISGLKWHPIRTAR
                     RIFEIVYKRAGIKKDVAIHTFRPNLARSCRMSNLKGWIDDTVAYTAKLELKATLSSMN
                     ELYEMPKPAIRKQEV"
     gene            complement(7991..8347)
                     /locus_tag="PLF90_00445"
     CDS             complement(7991..8347)
                     /locus_tag="PLF90_00445"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOK55784.1"
                     /translation="MGSSINFKYLQMDNGSEFSRYFHQACKNLNLEHYFSRVRTPEDN
                     PEIERYNRTLEEEFLQMGNYIEDVDNFNRFLTDWLIEYNFNRPHHTLGYSIPMDFLSQ
                     MKGYKVLPIYSTCTNY"
     gene            complement(8870..9010)
                     /locus_tag="PLF90_00450"
     CDS             complement(8870..9010)
                     /locus_tag="PLF90_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55785.1"
                     /translation="MLVLSITLSPPPETFGYWFAVFHGIGYTILIFAIIVYLNKFRRR
                     NK"
     gene            complement(9362..9925)
                     /locus_tag="PLF90_00455"
     CDS             complement(9362..9925)
                     /locus_tag="PLF90_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55786.1"
                     /translation="MKNFLKKIEYLENKYIEYLKSKDKENLRREEEILKLKGSKSLSL
                     PQFQKIVKWKNPRDRSSAKNSERDIQKITKSCFEELKNNEYNDDKVRSAIEKLKKLNG
                     VGVAVASAILMAYDPCKFTPIDWRAWNMLYLLCYIDEPCPKSFTPDHYIKYLKVCRKL
                     AKELDVYLRTLDRALWIASKNYRNLDC"
     gene            complement(10018..10335)
                     /locus_tag="PLF90_00460"
     CDS             complement(10018..10335)
                     /locus_tag="PLF90_00460"
                     /inference="COORDINATES: protein motif:HMM:NF021560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HOK55787.1"
                     /translation="MIIRYNRESNTLDIWFGNPIDKFICGEIGDGIILKKDKDGNVIG
                     IEKLYISKRVGISSSPLEVMIDKTLDTQYISSIPPPGGLYIFSILRDTNKKLDFLTFN
                     FKI"
     gene            complement(10332..10541)
                     /locus_tag="PLF90_00465"
     CDS             complement(10332..10541)
                     /locus_tag="PLF90_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55788.1"
                     /translation="MIGKEEIVKETLKDPEEIRKSKIDENVLLFYKKFDKLYCVVVKC
                     YEKEGFIITCYPTEKIEEGEKIRKK"
     gene            complement(11494..13191)
                     /locus_tag="PLF90_00470"
     CDS             complement(11494..13191)
                     /locus_tag="PLF90_00470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOK55789.1"
                     /translation="MLKNFIKRINLVILIFFILLFFARELIVSSEKSITCDETVHIPA
                     GYFYVKNGDFFINFEHPPFSKILSGLFIIKQKIFFSEEAYRNLRKNEWDLGMFFFYLN
                     RENVDNIVFWARFPMILLGILLGFFIYLWSKELYGEIAGLFCLFLFSFCPNFLAHSCL
                     VTTDVPFTAFFVITLYYLWKFFETNNNKYLLFSGIFFGLSISTKFTGIVVIPFIILFL
                     IIMKIKDNFKTKLKSNLIFFLLYLVLIPFIVLLLTYRIYGFKNFLLGLKTIVFETTER
                     GHMSFLNGRFSTTGWRYYFILAFLYKTPIALIIFFILSIFINRKIEKKEFFLILPAFI
                     FFIFSSFSKKQIGIRYILPFYALIYIYCGRLCVYHEKIKIKPVFKKVILIILIFWYAF
                     SSFKIHPHYLAYFNEIAGGPENGWKHLIDSNIDWGQDLKGLKEYLQKEGKPEIMLNYF
                     GSILPETYGIVYEPFFYPLFLNLPQQYYRLNSEFPQKEYLVISVNYLQGLVYNDHNIF
                     NWLKEIKPRIKIGYSIFVYDITEDIFIRTKLFEMFDKYGFARQAERQKKIIDRIKNK"
     gene            complement(13185..14195)
                     /gene="hypE"
                     /locus_tag="PLF90_00475"
     CDS             complement(13185..14195)
                     /gene="hypE"
                     /locus_tag="PLF90_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOK55790.1"
                     /translation="MKEDRIILSHGSGGKLMHSLIENIFLKNFKNDILEKLYDSGIIE
                     FEDFDLCFTTDSYTVEPVFFKGGDIGKLSICGTVNDLAVCGAKPLYLSASFIIEEGFE
                     IKNIEKIVKSMEKIAREADVKIVTGDTKVVEKGKIDKIFINTAGIGIKEKRIKLGIER
                     IEEGDVVIINGGIAEHGLSVLLSRDIYDIESEIKSDCAPLNKLISEILKETDGIKFMR
                     DPTRGGISATLNEIVRNRDFGIVIYENKIPVKKEVLSMCEILGFDPLNIANEGKVIII
                     CSRKDSEKVIKKMKKNIYGKKAEIIGEIVKRPEGKVLIETLSGARRIVDMPVGEQFPR
                     IC"
     gene            complement(14182..15276)
                     /gene="hypD"
                     /locus_tag="PLF90_00480"
     CDS             complement(14182..15276)
                     /gene="hypD"
                     /locus_tag="PLF90_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOK55791.1"
                     /translation="MEYIKEYRNTEKQKEIITEIQKYSKILKKKINIMEVCGTHTMAI
                     GKYGIRKLLPENVNLISGPGCPVCVTPEEYIDTAIELSRDKDVFITTFGDMMKVPGSF
                     SSLEKERANGAKIFVVYSPLDALKVSYENKNKKVIFLSIGFETTTPLISQTVKIAREE
                     KIRNFFVLSGNKLIPPVMEFLLKQKDTEIEGFICPGHVSAITGIEPYKSISKKFNVSC
                     VISGFEPLDILLSILLILKSINEGKSEVINEYKRTVREEGNIKAKKTIYDTFKIIDSE
                     WRGIGIVEDSGLKLKEEFIDFDAEKEFVIKIKSSGKKKSCLCGEILKGKKIPFDCPLF
                     EKICNPENPVGPCMVSSEGTCSAYYKYERR"
     gene            complement(15264..15482)
                     /locus_tag="PLF90_00485"
     CDS             complement(15264..15482)
                     /locus_tag="PLF90_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOK55792.1"
                     /translation="MCLAIPMKVEEIEGDYAVVSSGNVKRKVNISLVENVKKGDYLII
                     HAGFAIEKLDKKEAKKTLNIIKEMKWNI"
     gene            complement(15496..16593)
                     /locus_tag="PLF90_00490"
     CDS             complement(15496..16593)
                     /locus_tag="PLF90_00490"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOK55793.1"
                     /translation="MRKYFVFIIVSLFLSGCICEKIEEKKKLEELPEVYFPSEEKTEE
                     SKIEITKEEKKEEEIVEKKKEEEVKEEKKEEGVVQKEEVEGIKEEKTEEKEEIVLLPK
                     KVVFPKKEEKITGVPQPFFVNYLGECLIYELKWNFVNLGKAIIVCLEEKDRYRLVGIT
                     LPSGVPAQIGYGYNRVDSFIDKKTGKTSYFYIYSKTGDKEQITDLYFNWNANQYTCIT
                     KTYKNKNLLSTKREVINFDGEIFDCLGFFYFLRNGDINTFKNNQFPIALKEKWYLKIN
                     FKGKEVKKLPNGESKEVFILEPLARKEKESFKKGIIDIWITNDSEREPLFFEGRLPIG
                     KAVMNLTKVIKLEQKSDINVNKVLNEIVSTL"
     gene            complement(16646..16993)
                     /locus_tag="PLF90_00495"
     CDS             complement(16646..16993)
                     /locus_tag="PLF90_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOK55794.1"
                     /translation="MINKREDMEFEEREKMRGGEGIIKILHFFKKEDFKANVRLCAKL
                     IIEPGCSIGLHQHLDEDEVFIILKGKGIMYDGKNEYSVKEGDAILTGNGEQHSIKNIG
                     KDNLEIIAFISKY"
     gene            17039..17932
                     /locus_tag="PLF90_00500"
     CDS             17039..17932
                     /locus_tag="PLF90_00500"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOK55795.1"
                     /translation="MRKFKLLLNILPLLFISLKPVGYLNDFADTLSDEQKEKIEKRLK
                     DIEKETTNEICVLIIKSLEGKSIEEYANEIFNNWGIGKKGKDNGVLILISLDERKIRI
                     EVGYGLEGYLPDSVCGRIIREIMAPNFKRGDFYAGIYEAIEKIYMTTKGEEVVERENI
                     PPAGFRLLWYTFILLFSFIILGLIGFLFEAVLISFFLINAFLNRNNISQYGLFIFLSL
                     LIPFFFIFILGFISNLLFQNIRRRLKKYYGNRWKEHVPFYLRGSSIYSSGSFHGGGGF
                     SGGFGGFGGGASGGGGASGRW"
     gene            17950..18594
                     /locus_tag="PLF90_00505"
     CDS             17950..18594
                     /locus_tag="PLF90_00505"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOK55796.1"
                     /translation="MEEKIKTFVEKTKEIFKERLKSCIIYGSYVKGNYRKGISDINLI
                     VIVDSLSKTDLEEIKKRLSKFAYKNMIRFYLFSEWFLLSSADVFPVEWLDIKENHRVI
                     YGEDITEKIKVEKENLRVEIERKTKQLFLDFQHSIIFERNIYSVIEDVIKNLKFLIPL
                     IEKQMGKRIAIPEGLEIFEKNVKLKKKEIYEKVDEILKFLSEIIYLINERKEKK"
     gene            18591..19154
                     /locus_tag="PLF90_00510"
     CDS             18591..19154
                     /locus_tag="PLF90_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOK55797.1"
                     /translation="MKRNLNVLLWTILLIFIFGIFVYQYNRLVYLDQKVKSSWAQVEN
                     VYQRRNDLIPNLVEVVKGYAKHEKELFIQVTEARTKWQNAKSISEKETAAGEIDRVIG
                     RLLLVVENYPQIKASTNFLSLQDELAGTENRIAVERRRYNIAVEEYNRIAKSFPTFIF
                     VKIFGFDKEKSYFQAEKGSEKAPEIKF"
     gene            complement(19151..20047)
                     /locus_tag="PLF90_00515"
     CDS             complement(19151..20047)
                     /locus_tag="PLF90_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOK55798.1"
                     /translation="MKKIIFFLNFIVSLVSGETEVLFTPSKKTYSYFINLLQNAKKSI
                     YISTYSISPDFLNFLKGKKLDIKIVCEYGEDKNLQIKKIENKNLFHSKFLIIDEEVSI
                     ITSANLTFSNFYKNHNNLVIIKDKNLSRYLIKKFDSFWNNYNYTENFKDRNIEIWFSP
                     ENDCEGIIEREIKNSTNSINFATFSFTDTNIAEKIIEKRNEGILVKGIIESYNIFPHS
                     VFYLLLSYGCDVRKSCISGFLHDKFFIIDREKVITGSFNPTKSAKENVELLCLINDRN
                     TAEEFYREWRYLYLFKSTKEDI"
     gene            20114..21397
                     /locus_tag="PLF90_00520"
     CDS             20114..21397
                     /locus_tag="PLF90_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOK55799.1"
                     /translation="MYEIRKGKGDYEWFIRAKFGLFIHWGLYSLPARHEWVRNREEIP
                     NEIYDEKYFKRFNPDLYNPEEWAKIAKKAGMKYAVITTKHHEGFCLWDSKLTDYKATN
                     TPCKRDLLRDFVEAFRKEGFKIGFYYSLIDWHHPHFTIDRFHPLRNHPEKEKLNKERD
                     MAKYREYLHGQIRELLTEYGKIDILWADFSYPGPDGKGKNDWNSEKLVKMIRELQPHI
                     LLNNRLDLEDEYDFVTPEQVVPNEWPEKDGKKIIWESCQTFSGSWGYHRDEATWRDTE
                     EIIKTLIDCVSKGGNLLLNVGPTARGEFDYRARERLEKIGEWMKYHFSSIYDCTKAPE
                     EFKIPENCKLTYNPEKNRLYVHILSWPYKFLVLEGKAYKEQVEYAQLLNDGSEIKIGT
                     DEWHKKQLGEKEGKILTLPPFKPNVAVPVIELFLK"
     gene            21415..22947
                     /locus_tag="PLF90_00525"
     CDS             21415..22947
                     /locus_tag="PLF90_00525"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOK55800.1"
                     /translation="MKEILYRRNIKEEKEIKKFLFPTLNDISGPEIISHIEKASIEIL
                     NGIKKNEKIFVYGDGDIDGIGGVFFILKFLKEKNGDFNYYLTHRLDDYEIEENLVDYL
                     LNENYKIIILIDCGVSSYNFLKKCVENNIKTIVVDHHQTDIENLPEGPIYIHPLFSSE
                     KIDLSATGLSFKLFQYLMSNYSHFCLNDYICIVGLATLSENLNLTDENRIFVKEMLKD
                     LKYSNIKGLNLLVSKYLKKSILEIDEVKIKINPKLNSPGRFGKPEITLNLFLEEEEKE
                     IEKLLQEIEQLDKKRYKITQEIIKKIEEKENFEERFLVFETLPESLCGIIASRLTEKY
                     GQPFFIASKKGNILKGSARSPENYDLYTKMKKIKDKFLSFGGHKTAIGFKFSLNNEKE
                     IREYWKSIKIEKNQDDEYFDIVFDIEDIKPEIFEYLNLLKPYGKGNRPPVFLCRDVSI
                     KEIKKGKEQKYWIKKGNTIFESIFETRKKPNGEIKEIFYTPEIKEIDGYYKISLKIKN
                     FR"
     gene            complement(22940..23146)
                     /gene="rpmB"
                     /locus_tag="PLF90_00530"
     CDS             complement(22940..23146)
                     /gene="rpmB"
                     /locus_tag="PLF90_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOK55801.1"
                     /translation="MAMRCEICGKEGRSGNKVSHSNRKTRRTWKPNIQKVLVEYKGIV
                     KKMRICTKCLKTKVKRPIKEVQIT"
     gene            complement(23151..24929)
                     /locus_tag="PLF90_00535"
     CDS             complement(23151..24929)
                     /locus_tag="PLF90_00535"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOK55802.1"
                     /translation="MKEKEIARYKVKQYKGKGPKLKTKREIEAYYWSGVFVFFMGVLL
                     IFKPAFDYTLIKNLNGLLFCLLISIYFLFSKKEFEISNFIYLPVIFFTYVLISCFYAP
                     FEFEAAKNLENYLLYFLIFIISSNLEIDKKIFYIWIGAGIIASITGICQFYGERHYAV
                     STFGNPNFFSGHIIMVLCLSFSNILNWKLYEKEKYSGLFKLLDISCLIFGLWALGVAQ
                     SRAALMAFVIGLSSVWYIHNIENRSEIKYAVIFLIIFILAIFFPKIILWYKTNIRSYI
                     WKGTWRMILRKPVFGWGFGNFIFFYPYFRVREYFLQPESTPITNHPHNEYLEIWSEMG
                     IIGLLIFLIFVFSFIVGAIKNKGSKIFFSGIIGGIISVLCDNIFSTNLRNPSTSMYFW
                     FLIGITSKYTKVKKIDFKFSKYLWYIIFSVSLIFSIFYSYYRIYPQIYYKNGIGAKDL
                     GDYEEAIENYLIVCKLNPYNYECWYKLAYAYGMVGNYKKAEEIYLHINENLFPHYAKT
                     DANLGTVYLKVGEIEKAFKYYKEAEFFNPYDEDILCSIASIYLVYYNNIEKAVEYLQR
                     VLKINPDNIYANRTISLLKKEGKIRR"
     gene            complement(25271..25819)
                     /locus_tag="PLF90_00540"
     CDS             complement(25271..25819)
                     /locus_tag="PLF90_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HOK55803.1"
                     /translation="MIKRSKVEELKKKSFEMLKKAGIVITEDEYKNMEVVDAGLGIPD
                     EIGLQIIVYVNNERYCAKELVLLPRQTCPEHRHPPISEKNPGKQETFRCRYGEVYLYI
                     PGEPVENPKAILKSRKEYFTVWHEIILKPGQQYTIPPDTLHWFQAGDKGAIVSEFSSQ
                     SIDETDIFTDPDIKRITEIEQG"
     gene            complement(25812..26192)
                     /locus_tag="PLF90_00545"
     CDS             complement(25812..26192)
                     /locus_tag="PLF90_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HOK55804.1"
                     /translation="MVITIILLNRRLGMTTILQKRKGKLETLKQNWKENLPELPEINF
                     DRTPEESFKEIKDLELRYWRKILENEKLWEEGIMNVLFKEGKTLSLLYNFFREKEITP
                     YKNLETMLVEKLKKYWIKGGEKND"
     gene            complement(26215..26421)
                     /locus_tag="PLF90_00550"
     CDS             complement(26215..26421)
                     /locus_tag="PLF90_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55805.1"
                     /translation="MKKLAEKPERFIGLFRPTKPGAKILQHLLQSHEIRLGDAVEEII
                     DEIFNEIGFKSLNKKVINNKTGEE"
     gene            complement(26584..27408)
                     /locus_tag="PLF90_00555"
     CDS             complement(26584..27408)
                     /locus_tag="PLF90_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOK55806.1"
                     /translation="MKKIINGDLFIEQKEEMIIKDSPEKIHYTTTEIKNKVILGDCFN
                     VLRKLREESFDMVFIDPPYFLQLPQKELRRWKVKSIVEGVNDEWDKFSCFEEYDKFIA
                     NLLIEVKRVMKSNATIWVIATYHSIFRIGKIMQDLGFWILNDVIWVKTNPMPNWLGVR
                     FTNATETLIWAVKDKNVKRYTFNKEYAKKFGIGTIGANVWVLPICWGKERIKDYNGKK
                     LHSTQKPIELLKRVILTSTKERDLILDPVAGTGTTGYVAKGLNRNFVIIEINQKYV"
     gene            complement(27405..28841)
                     /gene="gltX"
                     /locus_tag="PLF90_00560"
     CDS             complement(27405..28841)
                     /gene="gltX"
                     /locus_tag="PLF90_00560"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOK55807.1"
                     /translation="MVRVRFAPSPTGFLHIGNARTALFNYLFARKNKGKFVLRIEDTD
                     IERSRKEYEEAIIEDLKWLGLEWDEGPDVGGEYGPYRQSERIYIYREFAEKLLKEGFV
                     YKCFCTKEELEEMRKQAQEKGLPPGYDGRCRNLTKEKISQYEREGRQYVLRLKVPEEK
                     IEINDLIRGRVVFEGEHLTDFVIMKSDGTPTFNFAVVIDDYLMRITHVIRGEDHLPNT
                     PKHVLIFKAIKAEIPQFAHMSMTLGPDRTRLSKRHGATSVRAYKEEGYLPEAFFNYIS
                     LLGWGTTESKEIFTKEELINEFSIERCHKASAVFDPEKLLWMNSLYIRNSSIDRIYQL
                     SLPFLKEKGLLYDNVEREKEEYIKKAISLEQEKIKKLKEVPYLIEFFLKDPEYEKENF
                     EKLINENTKKILSEIADVFEKMDDFKREEIERKVRDYCSEKGLKTSFVFHPLRFVVSG
                     RTKGPDLFGMIELLGKEKVIERIKKVIK"
     gene            complement(28933..29997)
                     /locus_tag="PLF90_00565"
     CDS             complement(28933..29997)
                     /locus_tag="PLF90_00565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK55808.1"
                     /translation="MEIRQGNLEVLDEMIELADLVFRPKEKSMGIETPIMYSKENIEK
                     GNTFIAIENKKIVSFVGLVEEEINVYGNRMKIGEIGNVCTHPDYRGKGFASALLQKAI
                     EKATEDKIAYLMISGGRSLYKRIGAVSMLYYVYQIRGKNKKVDFKIKRFRDEMFSDCM
                     KIYQKEPVRFIRNKDFSQLFSLYNGKYRKKVMLCESRIYVIQKEREILGYWAEGERKG
                     VINIGYNREFAGSRKFLVEAWKSRRGKKNIFVPLWDRELHYLLGKEKEKREMGTILLI
                     NPEILFSSLYDFFRERNIEIEYRTEDNKFLLKAYNEEKKFENLTTFSEFLFNFHEENQ
                     NGIWKKILPIPLPSYGLNYA"
     gene            complement(30011..31270)
                     /locus_tag="PLF90_00570"
     CDS             complement(30011..31270)
                     /locus_tag="PLF90_00570"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOK55809.1"
                     /translation="MNNLTPLEFLDSLSDFGIKLGLDKTIYYLEKLGNPHYKYPSVLV
                     AGTNGKGSVCKTLSKILQTSGYRVGLYTSPHLIDVKERIRINEEKIKEKDFIEKIEQL
                     RKLIEKQPYHLYPTYFEALTIIAFSYFYDKKIDILVCEVGMGGRFDATNVLPSFLEII
                     TKIGLDHTEFLGKTYKMIANEKAGIIKEKTMVISAKQKKDAIDVIKKKVKDKKGELFI
                     YGEDFKGRIVSLSPSGMIFDFNGREKYKNLKTDLLGTHQVENLSIAIEAGLILKEKGY
                     MIKEKDIREAIKNINWKARFQILQKNPYIIFDGAHNVDGVKNLMKNVKKIFPDTRFNY
                     LVGILKDKDYKNMVKIFEKHAGKIILTKPNSDRAIEPSVLKGLLKNKKNVKIIENPEE
                     AYKYIKGTKENWLIFGSLYLASSILTE"
     gene            complement(31267..32109)
                     /gene="accD"
                     /locus_tag="PLF90_00575"
     CDS             complement(31267..32109)
                     /gene="accD"
                     /locus_tag="PLF90_00575"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOK55810.1"
                     /translation="MIFKRKRYIEISPKEKVEIKKELWVKCEKCGKLIYTKKLEENLK
                     ICPECNFYFRMTAKERINSLIDAGTFSEKWGDLQSEDPLNFVGVKSYKEKLEEEMKNT
                     GMNEAMVTGTGKIGNFPVAFCVLDAHFIMGSMGSVVGEKFTRLCEFAVENGLSLISVS
                     GGGGGARMYEGVVSLMQMAKTSAAVGKLKKAGILYVSILTDPTMGGVAASFAFLGDII
                     IAEPKALIGFAGPRVIEQTIKQKLPPGFQTSEFLYEHGLIDMVVERKNLKETLIKILR
                     IFHS"
     gene            complement(32106..32981)
                     /gene="dapA"
                     /locus_tag="PLF90_00580"
     CDS             complement(32106..32981)
                     /gene="dapA"
                     /locus_tag="PLF90_00580"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOK55811.1"
                     /translation="MFKGAIVALVTPIKNDKVDYNSYKTLIDFQIKNKTNGILVAGCT
                     GEPATLSYEEHKDLIKFTVEYVNKKVPVIAGTGSNNTKEAIELTEYAEKVGADAALLI
                     TPYYNKPTQKGLYLHYKAIAKAVSIPLILYNVPSRTGISITPETVAELSEIKNIVGIK
                     EASGSLDQVSKILTLLKREDFTVLSGDDSLTLPIMSVGGKGVVSVASNIVPLEVSQMV
                     EYTLNGDYENARKIHLKLFPIFKILFIETNPIPVKTCLSLMGMISPEWRLPLCGPSDE
                     SIEKIKKTLKESGVL"
     gene            complement(32995..33276)
                     /locus_tag="PLF90_00585"
     CDS             complement(32995..33276)
                     /locus_tag="PLF90_00585"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOK55812.1"
                     /translation="MKTEKIDSIVKRVLNKIEIPDEKNRNIDITVLWPRIIDEKMRGK
                     SYVLYEKEKKLYIKVENSCFLSLCRMNKKKIIEKLKEFGFNYIDIKFLI"
     gene            complement(33280..34419)
                     /gene="dnaN"
                     /locus_tag="PLF90_00590"
     CDS             complement(33280..34419)
                     /gene="dnaN"
                     /locus_tag="PLF90_00590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOK55813.1"
                     /translation="MKVNISSEVLNKGLEYISDVLPAKPTIAICGGIFLEGKENNLTL
                     IATDLENTVKVSLECDVKEKGQCVIPGKQFISLLKQFKNKEIKIEKKEKFINLIGESS
                     QYSFIEMETEDFPKIPKFSSEITFRIKGETLKDGFKKTIFCIDPDEPRQQFRGGLFDI
                     KEKVINIIGTDTRRLSLFSILFDTPFKKNTKVLLSYNLIKKMINLLNNEEVEVFIGKN
                     NISFQTSFTIQMEENKKGIGSVLFISQLLTGAEEFPDYEKVIPDIKKSKISQINTDLF
                     LSSLKRISLFTSERNNKVKLSFKKDALILSASGELGEAQEKMDIPYSDEEIDIIFPPN
                     FLIDFLQVVEKKEFIFSFTSSSKPVLMKPEEEDNFLYVCMPLKTE"
     gene            complement(34536..35849)
                     /gene="dnaA"
                     /locus_tag="PLF90_00595"
     CDS             complement(34536..35849)
                     /gene="dnaA"
                     /locus_tag="PLF90_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOK55814.1"
                     /translation="MEKEVHKLWNIVLNNVEEKINNSRTFDLWIKPVKLIDITENIVK
                     IEVPNSTFEKNFLPYIEILREEIQKIFGFNPNFEIIYSIDLPSEIKEESFFEIALNPE
                     YTFENFVVGPCNRLAHAASVAVAQSPGVAYNPLFLYGGVGLGKTHLMQAIGHFVKEKS
                     NAKIAYMPSEYFVNEFITSIKNKTTHLFRNKFRNLDYLLIDDIHFIAGKEGTQEEFFH
                     TFNFLYDKRKQIVLSSDKPPKEIKFLEKRLVSRFEWGLIVDLQTPDIETRVAIIKKKS
                     ELRKLSFSEDLIFYIAEKIKDNIRLIEGILNGLVANVTLLNRELNKNLIDEIIEGVYS
                     SDKKEKKFVNIKQIMNVVCEYFGLSPEEIKSEKRVKNIILPRQIAMYLGRELTETSLN
                     SIASEFGGKDHTTVLHACKKVKELMEKDKNLKKIIENIKEKLNEQ"
     gene            complement(35885..>38572)
                     /locus_tag="PLF90_00600"
     CDS             complement(35885..>38572)
                     /locus_tag="PLF90_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOK55815.1"
                     /translation="GRRWSDGLHQAIEAKEGVRIESENQTLATISFQNYFKLYKKIAG
                     MTGTAITEALEFKEIYKLDVVVIPTNKPLNRKEYDDEIYSTEKEKFNAILKEIEELYK
                     IGRPVLVGTISIEKAEKLSKMLDRKNIPHKVLHGKNHEAEAAIIAQAGKPKAITIATQ
                     MAGRGVDIILGGNPEIIAREETVKILWARKREKKHIKSDFIDIVNEIEEKYKKRQEEI
                     EKTIGTQLKEFKEKLSEIEKNLYQKEKVVKEIFEKEIFEKKSKGLFKRYENKLLKLKA
                     IYEANNNKLQELNIQFKDKPEKTKEIRETTGKAFREYINYKNYIKNLFDIKTKENIEE
                     QRSRLIEIIENEKNEKELINSLITLLEVIKNTCISCIKTLEEVYPEILFTEEAEKSKK
                     KINRYIEFIDNLKNQISEKTQIEQIVSDFKNREYHVYKGLEEGFTEIEREIYKKYSPD
                     YLEIEKEYEKTVREYEKIQKKYIEELNLAREEYERERSQYEEEWKKIREDMEKSPEEF
                     KEIYQELLEKYKKPWEEDHKKVVELGGLHVIGSERYEARRIDNQLKGRAGRQGDPGSS
                     KFFLSLEDDLLRIFGGERLKGLMGKLPEGEKITHPLITKMINNAQKKVEGRNFEIRKN
                     LLEFDNVLNEQRNIIYTLRQDILENRNLDEYLKEYIEEIIERVFNEYCDEKIKPYMWN
                     IEGLTKYFKTTFGINIEIENPENIKNYLLWKDDFKEKLKDIFISVLEDKKKQLGEFFE
                     ELKKFVFLQVIDNRWKAHLRAMDELREGISLRAYAQKDPLIAYKHESFQYFQEMLKSI
                     KEEIIGNLLKIQVTEKVALKVRETERKEFVHQTYEQFATTKEKEEPVSVAQSISEGLY
                     NKNVINSPTASLKKQKIGRNQPCPCGSGKKYKHCCGKNE"
BASE COUNT        12305 a         6622 c         4729 g        14916 t
ORIGIN      
        1 acctgctgat atcgctacta catcttctaa accttctgga acattacatt gaccacacaa
       61 attacttccc catgctacta ctttcacatc tttctttaat gctactgtat gactcccacc
      121 tgctgatatc gctactatct tacaataacc actgaaaatt ccctcattca tcttgtcctc
      181 ctgttaattt taatttatca ttacatttat tctctatcaa atttattttt ttatacattt
      241 ttatctttct caaatttttt ccgaacccgg ttcggaatgt ggtaaatatt tactatgagt
      301 tacccaattg cctctttgct taaaactcca gatagttgtt tgttcttctt taaaatcttt
      361 tgggtgaagt gactttattt tttagaaact ttaaagaaac taatattaag cccttgagag
      421 cgagtaataa gccattcaat atatctaaaa gtaactctgt acttttgacc aatttctaaa
      481 tcagtagttc cttttgtttt ttcatgtaag attgctttct caagttcttt tttagttttg
      541 aaacttatct tttggttagc tattttatcc tctttttatc aagcccacca tcttcgccta
      601 acgttcccag cataaaagaa gtttttgtga aacaaaaatt tgagtgcgtg ctgtaagaca
      661 tggatctttt atgctatgtt atatgaagtg gtttgtaatt caatcttttt atatctattt
      721 tttaactttt tggctttctc atcaaataac tccttaatat tatcatccat tgtcagatag
      781 ataacctgcc acccagcctt tgcaaggtca acaagggatt caactaagat aggccttctc
      841 tcatagtctg aatgctgaaa agcatcatca agaatgagaa atgcatggtc ttctcccata
      901 actatctttg caaaacccat cctcaaagca aggataacct gttcctttgc accagtgctc
      961 agatctttta gtggaaaagt ttcgtaatca ctgctaacca ggagattgcc gtcatcatca
     1021 aacgatagct tgttataccg tttcgtgagg gttaaaagaa gtttttttac ttcttctgaa
     1081 ttcattactt caacaatttt ttcattttct tctctgagaa gctcttcaat ggtctcacta
     1141 accagcttgc ctgctattat atttgactca acttcattca attctctgac aatttcttct
     1201 tttttcaaat atagtttttc tatcaataaa ttccaatctg aactgatatc tgaatctgta
     1261 aaagaacaaa tttcggattt caagtttaaa agtttttctc cctcattttc acgggttttc
     1321 ttaagttcat taattctatt ctcaatttct tttatcttcc caggagaaaa ttcaaccccg
     1381 gggttctgtc tttcatattc tccttccgaa actccaagtc ctctgagttc accttccttt
     1441 tctttcatat ttttaagggt atcatttctt tttttctcaa tctcctgtat ttttcttttc
     1501 cactcatgtt gattcacttc ctcaccgata atctcagaga acgcttgttt aatctctttt
     1561 tccaaatctt tcagttcatc attaaatttt tggaattcat tggatttttc ttcctcttgc
     1621 cgttttagtt ccctcagtct ctcgacttct ttacttattc tctcaaattc agatttttca
     1681 aatttaattc ctggattttc tttcacaaaa tccgtttcat ctacatctaa cttgttgaat
     1741 tcttttttta agtcttcaat attctttgat atatcatcct ttttattttt tagttcagaa
     1801 agcatatcaa gccagttttg ttcgttaata tcagtgccta ataatttttt gaacttatcc
     1861 tgtattgaaa ctttgaggtt ttgaatattt tcctgaatcc gtgatatttc gtctttaaga
     1921 tatactattt tgttataatc agtagtttgt tcattcaaaa tagacactat atccgcgaca
     1981 ttgttgagtt caaacccgaa atacttttta aattcatcac ttatgctttt aacttcttcc
     2041 ttctgtttta ttcctgcaag atatttttta atcccaatct tataccagag tatagaaata
     2101 cttgtcagta acataaatat agtgccaggt attctctgcc ccaataaaaa cagaacagta
     2161 gctatcataa gggacaatag agaaaggtaa gggagtgcct caataaattt tgtgaatctg
     2221 acaggtgttg atagatattt taaataatat tcctttgctt ccttaagcca ctcataatgc
     2281 tttgtctttt cctgtaattc gctcaacccg gatatcttcc gttgtagctc attatttttc
     2341 tcgttgtatc gtgagatatc cccctcaaga ccagataatt catcttcagg aatcttttca
     2401 agttctcttt ctttttgttt gatctcattc gcaagagaat atgctaggta tctctttgcc
     2461 ttcagaattt tttccagttt tctttctaaa ctttgcaaat tctcggtttc tttctttatc
     2521 ttatcgagtt cgttactcat ggatttgcgg cgctcctctc tcttattgaa ttcttcaatg
     2581 aagtttttta attctgaaat cttttcatca tgaggcagtt tttctatttt ctccttcagt
     2641 ttattgattt cttcggacag cagatatgcc ttgtatcttt ttgcttttaa caaattttct
     2701 ttcttttctt ctaattcttt tatctctatt tctatctttt ttatttctcc agcctcgtat
     2761 tgtttggtta catcctcaat aatcctattt actttttcga gcctttcttt aatttcatag
     2821 tatctctttc cttcacccat cttattaatt gatattcttt cctctatttc tgcgttttga
     2881 atagtggcgg agatattact ttttattgaa ttcaagattc ttttcccaga gagaatatca
     2941 tttatgagat ttttgtcgac acctgattca tttttaacta tttcaaattc accaccttta
     3001 acgacaagta gcctacagag agacggtggt aatcccctgg ggtctttttc aaaataatct
     3061 tctaatttct gttctttctt cgatcgtgat gatagtgaaa attcaacctc tctgccgcca
     3121 agtccttcga ggattacctt tcccctacta ctttcaatat ttctaaggta accccagttg
     3181 gaagtatttg tgaaaagaca ctttatcaaa aattcaacaa gaaatgattt cccttgttcg
     3241 tttttgccat aaataacatt caggctggct aaatcttcat caaattcttt tattggccca
     3301 caatttttca ctgaaatctt ttttattctc atttgttctc acttctaaat ataagttcca
     3361 tgactttttt cataatatcg tccgtagaag aaacacattc tcttttatca atacttttta
     3421 caatattttc tattttaatt tttactttgt taagtaataa tatcctttcc ctatccgttt
     3481 ctttaaggat tttaagttca gaagtatcgg gtcccgaact atcatagaat tcaattcctt
     3541 tatcacgaac aatttgcaca agattctgag tgataacatt tttgtctttg gtgtagcctt
     3601 tcaagaacag ccttagcttc acctttttta gttcatcttc gcttaactgc catttttgta
     3661 taatttcgta aaatctatct ttcaaataac tgatttcatc atccatagga tacattagta
     3721 ttgtttcatt aaaatagata acgtccgtct caactgtctc atattcaaga accagcgatt
     3781 gtgtatcata tatcaagaat cttcttttac ctgtttcatt aatatccagt ccacatggag
     3841 aacctggata aacgacttta ccgaattctg aatgtttgtg gatgtgtcca agtattacct
     3901 ttgcaggatt ataattgttt accgtcatag ccgtaagagg catataaaag ccaggttcat
     3961 agggatttag ttccttattt attgtgatat aatctccatg ccctattaat atccatcttt
     4021 ctgggaaagt gttacgatga gaaaactcaa caattacttc atccatagac ttttcaggat
     4081 tatatggaag aaaaagaatt ggaagtccat ctatttcttt tatctgtggt tcagtaatta
     4141 ctttgatatt tgtcgcagca aaaaacctgt tttcaatctt gtagtcatgg ttaccaggta
     4201 taatactaac actaacatta ggatatttct tacatagttc atcaaagtct gaatagttgt
     4261 tataatcctt atcaaataaa tcaccagcga taattaattc agaaatacct ttatgtgtta
     4321 tcttcgaaaa gatattttca agagcattat atctttctgg tgactctgaa cagttcttca
     4381 ggtgtacatc agaagttatt gctattttca ttttgcctca cattatttta gtcatttcat
     4441 ataacggccg cggacatacg aagtgcgagg caaagccgag catttgggtg agggcgtaag
     4501 cccgaaccgt atgtccgctg ttatatgacc gagccaaagg cgaggcaagg aaaccgaagg
     4561 tttccgtaga gtgcccaagc ccgcttcgtt tattttctat tttttttctt tctctcatgt
     4621 gcatcaacta ccgcctgcat aaactcaact gtttcatcaa ctgagtgatc cgccattgcg
     4681 acattacacc agcggtgacc gtatcttacc aatctaggtg tatgtaatat tttgctttct
     4741 ttcagtggga atgtatgaaa caattgcaaa ctttctgctt cgccttcttc cctttttgac
     4801 tttctccatg gtggttggaa aacttcttcc ctctccggta aagataaatt cgcaggaggt
     4861 ttatttaatg gtgcacgaca taaaggacat aaaattatct cttgtatctc attttggttt
     4921 ggatattctc gagtaggatt gtaattggaa tttagaaaga tttctttgac caagttatag
     4981 gcatttaccg gatttacacc ataaaaattg ctgacaacag caataccgag tatagcaaaa
     5041 gtttctaccg aatattctaa tgttgaatat tccattgcag gtattacaca agaattatta
     5101 gtccagtcat catcagtcca ttgaagtaga tcatctttaa tagcatacca cttatcatat
     5161 ggaacacaat acacctcttc taacttgtga acatcttttg gataccaaag ctcgccattt
     5221 atgacaaata ctaatgattt gttatcaata ctaacatact caacttcctt tgcatactca
     5281 gggatttcat ttgctacaat aagagggtgc catctcaaat ttctgtttga gtaaggggct
     5341 ttatctgttc gacctcttgt acaaagagca tcccgctgga gtttggcacc tgccatacct
     5401 aaacctgcat ccctgcttaa atgaattctt ccataatttt ttacaaagtc cttcctactt
     5461 tctgtgtttg ttattaaatc agctagacca tcactgatct ttctacatat ttctaaggcg
     5521 gccttatctt tttccgtcaa ttccgtagct gaaatagtcc gacctttagc cattcttatt
     5581 ccctccctcc tgtttttatc agtttttcca tttttatccg tgcttctact ttcattttaa
     5641 taatttctga tacttcgctt attatttctt ccacagtact tttatctgta ggaattggga
     5701 ttacaacttc atacaacctt tctccaatag tagagatcgt agcttgaaca aaagtttttt
     5761 cttctatttg tttttgcaca atatcagtat tcaatagata gagtagcaag taaggatgca
     5821 gtttgtcagg ttttaaacat ctaattcttc ttatatgact ttgaattacc aattttttat
     5881 caagcgaagt aactattgcc gtccttccta ttagaaatgt accatcagta accaataaaa
     5941 tatcattttc tcttatatcc tgtctttttt tatatttttc gtaaacttct tctggaacac
     6001 attttatagg gtctattttt atttcccaat taacaatatc agacgttctt acaaagggga
     6061 tgttgccatt tccataatat tttgaaccta cctcatgacc tctttttaca gaaatccatt
     6121 tttgttctac caattcttta attgttgtaa gtttatattt tcctgttctc tccattttgt
     6181 taagaatttg cgtagtttca ggattataat aatttggaat gaaaataaaa tttcttacat
     6241 cggataattt tagagtaaat ccaagatgac tcagatggag gacgtttttt ataaattcct
     6301 tatacataga tgaaactaat ggtaggtcgt cgtctattat tttatttcct tcattatcaa
     6361 gaacataatt gcccttcata tccattttaa atactacttt accattttta tcatgcccga
     6421 ctttgtttgc aatagccatg aagatatcat agtctttctt aatattttgt gtcttttcta
     6481 aaaacaggac actcgttttc gtatgagtac ttggaagaaa ggtttcaggg ggtaagctaa
     6541 ctattgctaa tatcttagca ttctttaaga taaattccca gacataccta taactaggat
     6601 ttccaaaaac cccatcagga agaacaattg ccattctacc atttggttta agaagttgga
     6661 gacacctttc tataaacagg atctgtggga cttggttaga aacaaaattg ctgattttcc
     6721 aattgttaga tttatctttt atccatttat gccctaattt atagttcttt aagatatttt
     6781 tatcttcaat aaatattttt gctccaaatg gtggattcgt aagcacaata tcaaaacttt
     6841 catctttaat tttttccttc atattttctg gccaagattc tggataaagt gaatcagcac
     6901 aaaatatatt tgcatgacca tttccaacaa tactcatgta tgctttacat atcttaacta
     6961 aatctatctc tttgtctatc ccgtacacat tacaaacatg tcccctttcc tttagatgtg
     7021 ataaaacttc aactaaaaaa ccaccagaac cacatgcagg atctatgact ttttcatctg
     7081 gtttaggatt caatatatca acgcacattt tgaccacgtt tctcggagta aaaaactgcc
     7141 ccttttctcc tcgcaatcca ggaccccaaa aagcttggaa tgcttcactt attacatctc
     7201 tttcactttc gacaagaaca tatccttgaa gtttttgtgc tacatattta atcgatttat
     7261 catcaagaag tatcttttct tctctatcaa agacatcagg atagctatcc tttacttttt
     7321 caaataattt tttgatccta ttactaactt ctttttcgtt ttcatctgct cttattatga
     7381 attctttgtc atttttgtta tgtgtttcgt catatatctt acaaaaaaga attctaatca
     7441 tttctgcgcc aagcctttct gctcttggaa tgttgctgtt actataaaga taataataaa
     7501 tttgcttgaa cacctcaggg agacccaaag gtaataatgt tggagtaaac aagtccaaag
     7561 gttttactac taaataactt tttctagcca tttttatacc tcttgctttc ttatagcagg
     7621 cttgggcatt tcatataact cgttcataga cgacagggtc gcttttaatt ccaatttggc
     7681 ggtataggcg actgtgtcat ctatccatcc ctttaggtta ctcattctgc aactccttgc
     7741 aagatttggt ctgaaagtat gaatggctac atctttttta atcccagccc gtttataaac
     7801 tatttcaaat atccttcgag cagttcttat tggatgccat ttcagtccgc tgatggtctt
     7861 aattttctca acaaatacag gattataggg aaaggtaact tttatcatac cttcatctgc
     7921 tttaagaatt tttatcatct gattcatatc tgattcatat tttaattata cttaattttt
     7981 ctctgctctg tcaataattt gtacaagttg agtatatagg taacacttta tatcctttca
     8041 tttgagacaa aaaatccatt ggaattgaat agcccagtgt gtggtgtgga cggttaaaat
     8101 tatactctat caaccagtca gttaaaaatc tattaaaatt atctacatcc tcaatataat
     8161 tacccatctg taaaaactcc tcttccagtg tcctgttata tctctctatt tcaggattat
     8221 cttcaggcgt cctcaccctt gagaagtaat gttctagatt aagattttta catgcctgat
     8281 gaaaatacct tgaaaattca ctcccattat ccatctgtag atatttaaag tttatactgc
     8341 ttcccattaa ggaaagggta gaaataaggt agaataaagg catccgaaag gggcgaaaac
     8401 gaaacaaact taagcacaca tctcccaaga aactctgcca ccccttttta tatcccttct
     8461 attttatcta aatcagtcaa cttttccaga tataaggagg tgttacctat ttttctcaac
     8521 ttctgtagtt ttgtaaaaag atgcttaaaa ccattgatac gtaaagaatt taaaaacttt
     8581 agaaaatttg acaaatttaa aagtgatatt tacatttctt tttaatatcc taatgacgga
     8641 ggacaatgct cggaagacat acaaaaaatt ctttttttta tccacttttt cagttttcaa
     8701 ttccttttct attgctttac ttgtccaata tccttcagat acttcaataa ctgctttttt
     8761 acaattcttt taactttatc cagccaacca taagtaatct ttcctatcta tcaactttct
     8821 tcacttttga actcattttg attgtggtat ctgcaatgtt gttaagatat tatttgtttc
     8881 ttcttctaaa cttattcaga taaacaatta tagcaaatat aagaattgta taacctatac
     8941 catggaaaac tgcaaaccaa tacccgaaag tttctggtgg aggtgataat gtaatagata
     9001 aaacaagcat caaggcccaa caaaaaatta gccatggctc ggtccatgta ggcgggtcct
     9061 tccaatattc ttttactggg taaggagttc cacaatgagg acaaatttct gcattacaag
     9121 caactctttt ttacatactt tacatggtgc taaataaatt tctgatttat ctttttctga
     9181 aaatttcttt aaatccttag agtaatttta gtggatatat ttttctaatc ccatttttct
     9241 taaatctgct ccacaatttg gacaattccc attttcgata tcttccactt taaggtcttg
     9301 gccacatttt aaacagaata attttctaaa ttccaattgt ttttaatact ctatttttcc
     9361 cttaacaatc taaatttctg taatttttag atgcaatcca aagggcccta tcaagggttc
     9421 gcaaataaac gtctaattct tttgccaatt ttcgacatac ttttaaatat tttatgtaat
     9481 ggtctggtgt aaaactttta ggacagggtt catctatata acaaagaagg taaagcatat
     9541 tccaggccct ccaatctatt ggagtaaatt tacaagggtc atatgccatt aaaatcgcgc
     9601 ttgctactgc aacacctact ccgtttaatt ttttcaattt ttctatcgca cttctaactt
     9661 tatcatcatt gtattcattg ttttttaatt cttcaaaaca gcttttggta atcttttgaa
     9721 tgtctctttc ggagtttttt gcagagcttc tatctctcgg gtttttccac ttgactattt
     9781 tttggaattg gggaagagaa agagattttg aaccctttaa cttcaatatt tcttcttctc
     9841 ttcttaaatt ctctttatct ttgcttttta aatattctat atatttgttt tctaaatatt
     9901 ctattttctt taaaaaattt ttcatttttt cctccataat tttcccccta ttttaaaaat
     9961 ctttcttttt caaagtccac tcttctatcg caatcccctt taaagcagct caggaaatta
    10021 aatcttaaaa ttaaaagtta aaaaatcaag ttttttattt gtgtctctca aaatagaaaa
    10081 aatatatagt cctccgggtg ggggtatact acttatatat tgggtgtcaa gggttttatc
    10141 aatcattact tcgagagggg aagaagaaat tccaactctt ttggaaatat acaatttttc
    10201 tattcctata acatttccat ctttatcttt ttttagaata attccatctc ctatttctcc
    10261 acatataaac ttatctatag gattaccaaa ccaaatatct aatgtattgc tctctctgtt
    10321 ataccttata atcacttttt ccgtattttc tcaccttcct ctattttttc tgttggataa
    10381 caggtaatta taaatccttc tttctcataa cattttacaa ccacacaata taatttatca
    10441 aattttttat aaaaaagtaa aacattttca tcaattttgc tctttctaat ttcttctggg
    10501 tcttttaatg tctcttttac tatttcttct ttccctatca ttataggatg ttttatttta
    10561 acaatatatt ctcagtactc ctctgaaatt ctgattttaa tatttaaggg gtctacttca
    10621 aataaaattt ttttcatttt aaattgtatg tttaaattat atacttggtt ttattttttg
    10681 tcttgattat accttaaaaa taacttttat caaaaaaact taaaaatccc attaaatcat
    10741 ttgtatatca attatttcaa ttaactgggt taaatttata caatatattg tatttttaaa
    10801 gattaaaaaa tacagcataa tgttgtgcaa gatttgtgca agatttatgc aaaaatatga
    10861 gaaaatatgg gaaaatgtgg gaaattgaaa aattgatgta agttatttat atttaataag
    10921 ataagcaatt tacaaggatt tttaaaattg gttaggaacg gattagcaat ccgccgcatt
    10981 agaccactat gctacccctc cagttattat tattttactt ttttctttat aataattcaa
    11041 tattctaaat gcttcatcat aaaaatctaa tttaaaaatt tttcaccccg agaaaaatta
    11101 agaaaaaaag agatgagaaa gaaattagat aatgaaaata ttacctatta tttttaccct
    11161 ttgggtcttt cttttattta ataaaaattc tttcttcttt atttcagttc gccacttatt
    11221 tttaaatcat atcttaaaat tttattttac ttcaattcaa atttttttaa aatttatcag
    11281 gtagagaaga aaaaataaaa gtgttagtta taagtattta aaactaaatt atataaaaga
    11341 tacttttctt ttttaaattt aaatatttca cttttaaaaa tttgacacct tcaagaaata
    11401 aaaataaaat attcagaaga gtatataaaa aataaaagaa agaattctcc ctatcccatc
    11461 ttaaaggtgt ccaatggaac aaaagaagta tctttatttg ttttttattc tatctattat
    11521 ttttttctgt ctttctgcct gtcttgcaaa accgtattta tcaaacatct caaataattt
    11581 tgtccttatg aaaatatctt ctgtaatgtc ataaacaaaa atactatacc caatttttat
    11641 tcttggtttt atttccttta gccagttaaa aatattatgg tcattataaa ccagtccttg
    11701 cagataattc acacttataa caagatattc tttttgagga aattctgaat ttaaacgata
    11761 atattgctgt ggaagattta aaaaaagagg ataaaaaaat ggctcataaa caattccata
    11821 tgtttcagga agaattgaac caaaataatt aagcattatt tctggttttc cttctttctg
    11881 taaatattct tttaatccct tcaaatcctg tccccaatct atatttgaat caattaaatg
    11941 tttccatcca ttttcaggtc ctccagcaat ttcattaaaa tatgcaagat aatggggatg
    12001 aattttaaaa gatgaaaaag cataccagaa aatcaatatt attaaaataa cttttttaaa
    12061 gacaggtttg atttttattt tttcatgata gacacacaat cttccacagt aaatataaat
    12121 taaagcataa aatggcagta tatatctaat tccaatctgt tttttgctaa aagaggaaaa
    12181 aatgaaaaaa ataaaagcag gtaaaatcag aaaaaactct tttttttcta tttttctgtt
    12241 tatgaagatt gaaagaataa aaaaaattat cagagcaatt ggagttttgt aaaggaatgc
    12301 aagtataaaa tagtatctcc agccggttgt tgaaaatctg ccatttaaaa aactcatatg
    12361 tcctctttct gttgtctcaa aaacaattgt ttttaatcca agtaaaaaat ttttaaaacc
    12421 ataaattcta taagtcagga gtaaaacaat gaatggaatt aaaacaagat aaaggaggaa
    12481 aaaaataaga tttgatttca attttgtttt gaaattatcc tttattttca taattataag
    12541 aaacagaatt ataaacggta taactacaat tccagtaaat tttgttgata tagaaagacc
    12601 aaaaaatatt ccagaaaaaa gtaaatattt attattgttt gtttcaaaga atttccacag
    12661 ataataaagg gttataacaa aaaaagcagt aaagggaaca tctgtagtta caagacaact
    12721 gtgagcaaga aagtttgggc agaatgaaaa taaaaataaa cagaaaagtc ctgcaatttc
    12781 accatataat tcttttgacc acaaatatat gaaaaaacca agtaaaatac caagtaaaat
    12841 cattggaaaa cgagcccaga aaactatatt atcaacattc tctctattaa ggtagaaaaa
    12901 aaacattcca agatcccatt catttttcct taaatttcta tatgcttctt ctgaaaaaaa
    12961 aattttctgt tttatgataa aaagacctga aagaattttt gagaaaggag ggtgttcaaa
    13021 atttataaaa aaatcaccat ttttaacata aaaatatcct gctggaatat ggacagtttc
    13081 atcacaggta attgattttt cagaagagac aattaactct cttgcaaaaa ataatagaat
    13141 aaaaaagatt aaaatcacca gatttattct ttttataaaa ttttttaaca tattcttgga
    13201 aattgttctc caacaggcat atctacaatt ctccttgcac ctgatagtgt ttcaattaaa
    13261 acttttcctt ctggcctttt cacaatctct cctataattt ctgctttttt cccatatata
    13321 ttttttttca tttttttaat tactttttca ctatcttttc tactgcatat aataataacc
    13381 ttcccttcat ttgcaatatt taaagggtca aaaccaagaa tttcacacat tgataaaact
    13441 tcttttttta cagggatttt gttttcataa atgactatcc caaaatccct gtttcttact
    13501 atttcattta aagttgcaga aattcctccc cttgttgggt ccctcatgaa ctttattcca
    13561 tctgtctctt ttaaaatttc agatattagt ttatttaaag gagcacagtc actttttatt
    13621 tctgattcaa tatcataaat atcccttgaa agcaaaacag aaagtccatg ttcagcaatt
    13681 ccgccattta ttattacaac atctccttct tctattcttt ctattccaag ttttattctt
    13741 ttctctttta ttcctattcc tgccgtattt ataaaaatct tatcaatctt acctttttcc
    13801 acaactttgg tatcacctgt aacaatttta acatctgctt ctcttgctat tttttccatt
    13861 gactttacaa ttttttcaat attctttatt tcaaatccct cttctattat aaaacttgca
    13921 gatagataaa gaggttttgc tccacagact gctaaatcat taacagttcc acagatagaa
    13981 agtttaccta tatctcctcc tttaaaaaaa acaggttcaa cagtatagga atcagtggta
    14041 aaacacaagt caaaatcttc aaattcaata atccctgaat cataaagttt ttcaagaata
    14101 tcatttttaa agttttttaa aaaaatattt tctattaaac tgtgcatcaa cttaccaccg
    14161 cttccatgcg ataaaataat tctatcttct ttcatattta taataggcgc tacaagtccc
    14221 ttctgatgaa accatgcagg gaccaaccgg attttcagga ttacaaattt tttcaaataa
    14281 tggacagtca aaagggattt tctttccttt taaaatttct ccgcataaac aacttttctt
    14341 tttacctgaa ctttttattt ttatcacaaa ctctttttca gcatcaaaat caatgaattc
    14401 ttctttcagt tttaaaccac tatcttcaac aatacctatc cctctccatt cagaatcaat
    14461 aattttaaaa gtatcataaa ttgttttttt tgctttaata tttccttcct ctcttactgt
    14521 tcttttatat tcattaatta cttctgactt tccttcatta atacttttca aaatcaaaag
    14581 aattgaaagt aaaatatcca gtggctcaaa accacttata acgcacgaaa cattaaattt
    14641 ttttgagatt gatttatatg gctctattcc tgtaatagca cttacatgtc ctggacatat
    14701 aaaaccttct atttctgtat ccttttgttt caataaaaat tccataacag gtggtataag
    14761 tttatttcca gaaagtacaa aaaaatttct tattttttcc tctcttgcaa tcttaactgt
    14821 ctgggaaatt aaaggggttg ttgtctcaaa accaatagat aggaaaatta cttttttatt
    14881 tttattttcg taagatacct tcaaagcatc gagtggagaa tatactacaa aaattttagc
    14941 accatttgct ctttctttct caagagaaga aaaacttcca ggaactttca tcatatcccc
    15001 aaaggttgtg ataaaaacat ctttatccct tgacaattca atagcggtat ctatatattc
    15061 ttcaggagta acgcagacag gacaaccagg gcctgaaatt aaatttacat tttcaggaag
    15121 caattttctt attccgtatt taccgatagc catggtatgg gttccacaaa cttccattat
    15181 atttattttc tttttcaaaa ttttgctgta tttctgtatt tctgttatta tttctttctg
    15241 cttttctgta tttctgtatt cttttatata ttccatttca tttcttttat tatattgagt
    15301 gtcttttttg cttctttttt atcaagtttt tcaatagcaa agccagcatg aataattaaa
    15361 taatctccct ttttaacatt ttctacaagt gatatattaa cttttctttt aacatttcca
    15421 gaagaaacaa ctgcataatc tccttcaatt tcctcaacct tcattggaat tgcaaggcac
    15481 atttttcccc tttatttaaa gagttgaaac aatctcatta agaaccttat ttacatttat
    15541 atcagatttc tgttccaact ttattacttt tgttagattc attactgctt tacctatggg
    15601 taatcttcct tcaaaaaata aaggttccct ttcgctatca tttgttatcc agatatcaat
    15661 tattcccttt ttaaaacttt ctttttcttt tctcgcaagg ggttcaagaa taaaaacctc
    15721 tttactttca ccgtttggca atttttttac ttccttacct ttaaaattta tttttaaata
    15781 ccacttttcc tttaaagcaa tggggaattg attattttta aaagtattga tatctccatt
    15841 acgcagaaaa taaaaaaatc caagacaatc aaaaatttct ccatcaaaat taattacttc
    15901 tctttttgtt gataataaat ttttattttt atatgttttt gtaatacagg tatactggtt
    15961 tgcattccag ttaaaatata aatcagttat ctgttcttta tcacctgttt ttgaatatat
    16021 gtaaaagtaa gatgttttcc ctgttttctt atctataaat gaatctactc tgttatatcc
    16081 atatccaatc tgagcaggaa caccacttgg aagagttatt cctacaagac gatatctatc
    16141 tttttcttcc aaacaaacta ttattgcttt accaagattt acaaaattcc atttaagttc
    16201 atagattaag cattctccaa gataattcac aaaaaaaggt tgaggaacac ctgtaatttt
    16261 ttcctctttt tttggaaaaa ctactttttt aggtagtaaa acaatttctt ctttctcttc
    16321 tgttttttct tcctttattc cttcaacttc ttctttctga acaactcctt cctctttttt
    16381 ctcttcctta acttcttcct cttttttctt ttccacaatt tcttcctctt ttttctcttc
    16441 ctttgtgatc tctattttgc tttcctctgt tttttcctca gatggaaaat aaacttctgg
    16501 taattcttcc aacttctttt tctcttctat tttttcacat atgcatcctg aaagaaaaag
    16561 agaaacaatt ataaaaacaa aatactttct cactctatct ccttttttaa aaaattatac
    16621 cctcaaaatt taaaaaattg aaattttaat attttgatat aaaagcaatt atttcaagat
    16681 tatcttttcc tatatttttt attgaatgtt gctcgccatt tcctgttaaa attgcatcac
    16741 cttccttaac agaatattca tttttaccat catacataat accttttcct tttaaaataa
    16801 taaatacttc atcctcatca agatgttgat gtaatccaat tgaacaacct ggctctataa
    16861 tcaattttgc acataatctt acatttgcct taaaatcctc ttttttaaag aaatgcaata
    16921 tcttaataat tccttctccg cctctcattt tttccctttc ctcaaattcc atatcttccc
    16981 ttttatttat catatttccc cctttgacaa gtccttataa aaaacttact atatttaaat
    17041 gagaaaattt aaacttcttt taaatatttt accacttctt ttcatttcat taaaaccagt
    17101 tggctattta aatgattttg cagatactct ttcagatgag cagaaagaaa aaattgagaa
    17161 aagattaaaa gatatagaaa aagaaacaac aaatgaaata tgtgttttaa ttataaaatc
    17221 acttgaaggg aaaagtattg aagagtacgc gaatgagatt tttaataatt ggggaatagg
    17281 taaaaaggga aaagataatg gtgtcttaat tttaatttca ttggatgaaa gaaagataag
    17341 gattgaagtt ggttatggac ttgaaggtta tttacctgat tctgtttgtg gaaggataat
    17401 tagagagatt atggctccta atttcaaaag aggagatttt tatgcaggga tttatgaagc
    17461 aatagaaaaa atatatatga ctacaaaagg ggaagaggta gtagaaagag aaaatattcc
    17521 tccagctggt tttcgtcttt tatggtatac atttatcctt cttttctctt ttattatttt
    17581 aggtcttatt ggttttcttt ttgaggcagt tttaatttct tttttcctga taaatgcctt
    17641 tttaaataga aataatattt ctcaatatgg gttatttatt tttttatcat tgcttatccc
    17701 tttctttttt atttttattt taggtttcat atcaaatctt ttatttcaaa atatcagaag
    17761 acgactaaag aaatattatg gaaatagatg gaaagagcat gttccttttt atttaagagg
    17821 ttcaagtata tattcttcag gttcttttca tggtggagga ggtttttcag gtggttttgg
    17881 tggctttggt ggtggagcaa gtggtggagg aggagcaagt ggtagatggt aaaataaaag
    17941 gagaaaaaca tggaagaaaa aattaaaaca tttgttgaaa aaacaaaaga aatttttaaa
    18001 gaacgactta aaagttgtat catttatgga agttatgtta aggggaatta tagaaaggga
    18061 atttctgaca taaatttgat tgtaatagtt gattctcttt caaaaacaga ccttgaagag
    18121 ataaagaaaa gattgtcaaa atttgcctat aaaaatatga taagatttta tttgttttca
    18181 gaatggtttt tgttaagttc agcagatgtt tttcctgttg aatggcttga tataaaagaa
    18241 aatcataggg taatttatgg agaggatata actgaaaaga taaaagttga aaaagaaaat
    18301 ttacgagttg aaattgaaag aaaaacaaag cagttatttc ttgactttca gcattctatt
    18361 atctttgaaa gaaatattta ttctgttata gaagatgtaa ttaaaaattt aaaatttctg
    18421 attcctctta ttgaaaagca gatgggaaaa agaattgcaa tacctgaagg tttagaaatt
    18481 tttgagaaga atgtaaaatt aaagaaaaaa gaaatttatg agaaagttga tgagatttta
    18541 aagtttttat cagagataat ttatttgatt aatgaaagaa aggagaagaa atgaagagaa
    18601 atttgaatgt tttattatgg acaattttgc ttatttttat ttttggtatt tttgtgtatc
    18661 agtataatcg tcttgtatat cttgaccaga aagttaaatc aagttgggct caggttgaaa
    18721 atgtatatca gagaagaaat gatttaatcc caaatctggt tgaagttgtt aaaggttatg
    18781 caaaacatga aaaagaactg tttatacagg taacagaagc aagaaccaaa tggcaaaatg
    18841 caaaatctat atctgaaaaa gaaactgcag caggagaaat agacagagtt attgggcgac
    18901 ttttacttgt tgtggaaaac tatccacaga taaaagcaag tacaaatttt ctatcattac
    18961 aggatgaact tgcaggaaca gaaaacagaa ttgcagttga aagaagaaga tacaatattg
    19021 ctgttgagga gtataataga attgcaaagt catttccgac ttttattttt gtaaaaattt
    19081 ttggttttga taaagaaaag agttattttc aggcagaaaa aggaagtgaa aaagcacctg
    19141 aaataaaatt ttaaatatcc tcttttgttg atttaaaaag atataagtat ctccattctc
    19201 tgtaaaattc ttctgctgta tttctatcat ttatcagaca gagaagttca acattttcct
    19261 ttgcactttt tgttggattg aaagaccctg ttataacttt ttccctatca attataaaaa
    19321 atttatcgtg cagaaatccg gatatacaac tttttcttac atcacaacca taacttaaaa
    19381 ggagataaaa gacagaatga gggaaaatat tataactttc aataattcct ttaaccaaaa
    19441 tcccttcatt ccttttttca ataatttttt ctgctatatt tgtgtctgta aaagaaaatg
    19501 tcgcaaaatt tattgaattg gttgaatttt taatttccct ttcaataatt ccttcacagt
    19561 cattttcagg agaaaaccat atttcaatat ttctgtcttt aaagttttct gtataattgt
    19621 aattattcca gaaagaatca aactttttta taagatacct tgataaattt ttatccttaa
    19681 ttataacaag attattgtga tttttataaa aattgctgaa agtcaaatta gcagatgtaa
    19741 ttatagatac ttcttcatca attattaaaa attttgaatg aaaaagattt ttattttcaa
    19801 tttttttaat ttgcaagttt ttatcttctc catattcaca gacaatttta atatctaatt
    19861 ttttgccttt aagaaaattt aagaaatctg gacttattga gtaggttgaa atataaattg
    19921 atttttttgc attctgtaat aagtttataa aataagaata agtttttttt gaaggtgtaa
    19981 aaaggacttc agtttcacct gatacaagtg aaacaataaa attcagaaaa aaaattattt
    20041 ttttcataac tataaattat aacaaagaaa gaaaaaatga taaaatagta aaaactaaaa
    20101 aggaggaaga aaaatgtatg aaataagaaa agggaaagga gattatgaat ggtttataag
    20161 agcaaaattt ggtcttttta ttcactgggg actatattct ttacctgcaa gacatgaatg
    20221 ggtaagaaac agggaagaaa taccgaatga aatttatgat gagaaatatt ttaaaagatt
    20281 taatccagac ctttataatc cagaagaatg ggcaaagata gcaaaaaagg caggtatgaa
    20341 atatgcagtt attacaacaa aacatcatga aggattctgt ctctgggata gcaaactgac
    20401 tgattataag gcaacaaata ctccctgtaa aagagattta ttgagggatt ttgtagaagc
    20461 attcagaaaa gaaggattta aaattggttt ttattattct ttaattgact ggcatcatcc
    20521 tcattttaca attgacagat ttcatccatt aagaaatcat ccagaaaaag aaaagttgaa
    20581 taaagaaaga gatatggcaa aatacagaga gtatttacac ggtcagatta gagaattgtt
    20641 aacagaatat ggaaaaatag atatactctg ggcagatttt agttatcctg gtcctgatgg
    20701 aaaagggaaa aatgactgga attcagaaaa acttgtaaag atgataagag aattacaacc
    20761 acatatttta ctcaataata ggcttgacct tgaagatgaa tatgattttg ttactcctga
    20821 acaggtggtt ccaaatgaat ggccagaaaa agatgggaaa aaaattatat gggaatcatg
    20881 tcagacattt tctggttcat ggggttatca cagagatgag gcaacatgga gagatacaga
    20941 agaaataata aaaacactta ttgactgtgt gagtaaaggt ggaaatttac ttttaaatgt
    21001 gggaccaact gcaagagggg aatttgatta tagagcaaga gaacgacttg aaaaaattgg
    21061 agaatggatg aaatatcatt tttcaagcat ttatgattgt accaaagctc ctgaagagtt
    21121 taaaatacct gaaaattgta aattgacata taatcctgaa aaaaatcgtc tctatgttca
    21181 tatcctttca tggccttata agtttttagt acttgaagga aaagcatata aagaacaagt
    21241 tgaatatgca caattactta atgatggaag tgaaataaaa atagggacag atgaatggca
    21301 caaaaaacaa ttgggagaaa aagaaggaaa aatactcaca ttaccacctt ttaaaccaaa
    21361 tgtcgctgtt cctgtaatag aattgttttt aaaatgatgg aaatccctta ttttttgaaa
    21421 gaaattttgt acaggagaaa tataaaagaa gaaaaagaaa tcaaaaagtt tctatttcca
    21481 actttaaatg atatctctgg acctgaaata atttcacata tagaaaaagc atctatagaa
    21541 atcttaaatg gaattaagaa aaatgaaaaa atatttgttt atggagatgg agatattgat
    21601 ggaattggag gtgttttttt tatacttaaa tttcttaaag aaaaaaatgg ggactttaat
    21661 tattacttaa cccaccggct tgatgactat gaaattgaag aaaaccttgt tgattatctc
    21721 ttaaatgaaa attataaaat tataattctt atagactgtg gtgtctcttc ttataacttt
    21781 ttaaaaaaat gtgttgaaaa taatataaaa accatagttg ttgaccatca tcaaacagac
    21841 attgaaaatt tgccagaagg accaatatac attcatcctt tattctcatc agaaaaaatt
    21901 gacctttctg caacaggcct ttcttttaaa ctttttcagt atttaatgtc aaattattca
    21961 catttttgtt tgaatgatta tatatgcatt gtaggacttg caactttaag tgaaaattta
    22021 aacttaacag atgaaaacag aatttttgta aaagagatgc tcaaagattt aaaatattca
    22081 aacattaaag gattaaattt acttgtttct aaatatttga aaaaaagtat tcttgaaatt
    22141 gatgaagtaa aaataaaaat aaacccaaaa ttaaactctc ctggcagatt tggaaaacct
    22201 gaaatcacat tgaatctttt tcttgaagag gaagaaaaag aaattgaaaa actgcttcaa
    22261 gagattgaac aacttgataa aaagagatat aaaataaccc aggaaataat aaaaaaaata
    22321 gaagaaaaag aaaattttga agagaggttt ttagtttttg aaactttgcc tgaatcgtta
    22381 tgtggtataa tagcaagccg tctcacagaa aagtatggac aaccattctt tattgcaagt
    22441 aaaaaaggaa atattttgaa aggttccgca agaagtcctg aaaattatga tttatataca
    22501 aaaatgaaaa aaataaagga taaattttta tcttttggag gacacaaaac tgctattggt
    22561 tttaaatttt cgttaaataa tgaaaaggaa attagagaat attggaagag tataaaaata
    22621 gaaaaaaatc aggatgatga gtattttgat attgtctttg atatagagga tataaaaccg
    22681 gaaatttttg aatatcttaa tcttttaaaa ccatacggaa aaggaaatag acctcctgtt
    22741 tttctgtgca gggatgtttc aataaaagaa ataaaaaaag gaaaagaaca aaaatattgg
    22801 ataaaaaagg gcaatacaat ttttgaatct atatttgaaa caaggaaaaa acctaatgga
    22861 gaaataaaag aaatttttta tactcctgaa attaaagaaa ttgatggata ttataaaatc
    22921 tctctcaaaa taaaaaattt caggtaatct ggacttcttt tataggtctt tttacctttg
    22981 ttttcaaaca ttttgtacaa atccgcatct ttttaacaat acctttgtat tcaacaagta
    23041 ctttctggat attgggtttc caagttcttc ttgtctttct atttgaatga ctaaccttat
    23101 taccgcttct cccttctttt ccgcatatct cacatctcat tgccatattt ttacctcctt
    23161 attttacctt cctttttcaa aagagatata gttctatttg catatatatt atctggattt
    23221 attttcagaa ccctttgtaa atactccaca gctttttcta tattattata atacacaaga
    23281 taaattgatg caatactgca taaaatatct tcatcataag gattaaaaaa ttctgcttct
    23341 ttataatact taaatgcttt ttctatttcc cctactttta aataaactgt tccaagattg
    23401 gcatctgttt ttgcataatg agggaaaaga ttttcattta tatgcagata aatttcttct
    23461 gcctttttat aatttcctac cattccataa gcgtaagcaa gtttatacca gcattcataa
    23521 ttatatggat tcaatttaca aacaataaga taattttcaa tcgcttcttc ataatcacct
    23581 aaatcttttg ctcctattcc attcttataa taaatctggg gataaattct gtaatatgaa
    23641 taaaaaattg aaaaaataag agaaactgaa aatatgatat accacaaata ttttgaaaat
    23701 ttaaaatcta ttttttttac ctttgtatat ttagaagtta taccgattag aaaccagaaa
    23761 tacatacttg ttgagggatt tcttaaattt gttgaaaaaa tattatcgca taaaacactt
    23821 attattccac caataattcc tgaaaaaaag atttttgaac ccttattttt aattgcacct
    23881 acaataaagg aaaatacgaa aatgagaaaa atcagaagtc ctataatccc catttctgac
    23941 catatctcaa ggtattcatt atgaggatga tttgtaatag gagttgattc aggttgaaga
    24001 aaatattctc ttactctgaa atagggataa aagaaaataa aattaccaaa tccccagcca
    24061 aaaactggtt ttctcagaat cattctccag gttcccttcc atatataact tcttatattt
    24121 gttttatacc ataaaataat tttgggaaag aagattgcaa gaataaaaat aatcagaaag
    24181 attacagcat atttaatttc tgacctattt tctatattat gaatatacca gacagaggaa
    24241 agccctataa caaaagccat taatgctgct cttgattgag cgactccaag agcccataaa
    24301 ccaaaaatta aacaggagat atcaagtaat ttaaataatc ctgaatattt ttctttttca
    24361 tacaatttcc agtttaaaat atttgaaaaa gacaaacata aaaccattat tatatgacct
    24421 gaaaaaaaat tgggatttcc aaatgttgaa acagcataat gtctttctcc ataaaactga
    24481 catatccctg ttatagaagc aataattcct gcgccaatcc atatataaaa gattttttta
    24541 tcaatttcca agttcgaaga tataataaaa ataagaaaat aaagaaggta attttcaagg
    24601 ttttttgctg cctcaaattc aaaaggtgca taaaaacagg atattaaaac ataggtaaaa
    24661 aaaattaccg gtagatatat aaagtttgaa atttcaaatt ctttttttga aaaaaggaaa
    24721 tagattgaaa ttaaaaggca gaataaaaga ccatttaaat tctttatcag agtgtagtca
    24781 aaagcaggtt taaaaataag caagaccccc ataaaaaaaa caaaaactcc actccagtaa
    24841 tatgcctcaa tctctctttt tgtttttagt tttggccctt tccccttata ttgtttaact
    24901 ttatatcttg ctatttcttt ttctttcatt tttatttatt gttcttttac tttaataaag
    24961 attttcctta attttcaaca gattttatta tatcataatt tattttttct aataaacggc
    25021 ttactttaat agaagaggat ttgaaaagat taaataggcc agagataaaa aaaatataag
    25081 tatgctatga aatttattga tatagagaac aaagaataca gaaaaaaaga agtaattgag
    25141 agattttcat atagattgac aataaagaag aatttataca ggaaggaaac aaaataaaag
    25201 agtatcataa agaaaaagtt gttaaaatca ttgatagaag ttggtgtaaa aatgaatgca
    25261 aaactcaagt ttaaccttgt tctatttccg ttattctttt aatgtcaggg tctgtaaaaa
    25321 tatctgtttc atcaatactc tgggaagaaa actcgcttac tattgcgcct ttatctcctg
    25381 cctgaaacca gtgaagtgta tctggtggaa ttgtatactg ctgaccgggt tttaaaatta
    25441 tctcatgcca gacagtaaaa tattcttttc tgcttttcaa tattgctttt ggattttcta
    25501 caggttctcc tggaatataa agataaacct ctccatacct gcatctgaat gtctcttgct
    25561 tccccggatt tttctcagat attggtggat gcctatgctc aggacatgtc tgccttggta
    25621 ataaaacaag ttcctttgca cagtatcttt cattatttac ataaacaata atctgtaatc
    25681 caatttcatc tggaattcca agacctgcat caacaacttc catatttttg tactcatctt
    25741 cagttataac aatccctgct ttcttcaaca tttcaaatga ctttttcttc aattcctcta
    25801 ccttgcttct tttaatcatt tttttcccct ccttttatcc aatatttttt taatttctct
    25861 accagcattg tttctaaatt tttataagga gttatttcct tttctctgaa aaaattgtat
    25921 aaaaggctta gtgttttccc ttctttaaat agtacattca taatcccttc ttcccacagt
    25981 ttttcatttt ctaaaatttt tctccagtat cttaattcta aatctttaat ctccttaaaa
    26041 ctttcctctg gagttctatc aaaattaatc tctggcaatt caggcaaatt ttccttccaa
    26101 ttttgcttca aagtttctaa tttacccttt cttttttgaa gaatcgtggt catccctaac
    26161 cttctgttca ataaaataat agttattacc atctgtaaaa tattgatcca aaaactattc
    26221 ttctccagtt ttattattaa taactttttt atttaaactt ttaaacccta tttcattaaa
    26281 aatctcatct attatttctt caaccgcatc ccctaatcta atttcatgag attgaagaag
    26341 atgttgtaaa atttttgcac cgggttttgt tggcctaaat aaaccaataa atctttccgg
    26401 tttctctgct aattttttca ataactcttt tttttctccc tcaaaaatat gcctatttaa
    26461 aattgaacaa aatttttcat agttcattgt ttacctcgat tttttcaaat attccttcca
    26521 ttctctatgt aactttaaag tttccgttaa ttttaaagaa ctttcaaatc ttttttttat
    26581 tccttataca tatttttgat taatttctat tataacaaaa tttctattta accctttggc
    26641 aacatatcca gttgttcctg tccctgcaac agggtctaat attaaatctc tttcttttgt
    26701 gcttgttaaa attactcttt taagaagttc tattggcttt tgagttgaat gaagtttttt
    26761 cccattatag tctttaattc tttcttttcc ccagcaaatt ggtaaaaccc aaacatttgc
    26821 tcctatcgtt cctatcccaa attttttagc atattccttg ttaaatgtat atcttttaac
    26881 atttttatct tttactgccc aaataagagt ttcagttgca tttgtaaacc taactcctaa
    26941 ccagttaggc attggatttg ttttcaccca tataacatcg ttaaggatcc aaaaacctaa
    27001 gtcttgcata atttttccaa tcctaaaaat actatgataa gttgctataa cccatattgt
    27061 agcattggac ttcattactc tttttacttc tataagtaaa tttgcaataa atttatcata
    27121 ttcttcaaaa caagaaaatt tatcccattc atcattcacc ccttcaacta tacttttaac
    27181 tttccatctc cttaactctt tttgaggaag ttgtaaaaaa taaggggggt caataaaaac
    27241 catatcaaaa ctttcttctc ttaacttcct taaaacatta aagcaatctc ctaaaataac
    27301 tttattttta atttctgttg tcgtataatg tatcttttct ggactatctt ttattatcat
    27361 ctcttccttt tgctcaataa acaaatctcc atttataatt ttcttcattt tattaccttt
    27421 tttattcttt ctattacttt ttcttttcca aggagttcta tcataccaaa caaatcaggg
    27481 ccttttgttc ttccactgac tacaaatctc aatggatgga aaacaaaaga tgtctttaat
    27541 cccttttcag aacagtaatc cctaactttt ctttctattt cctctctttt aaaatcatcc
    27601 attttctcaa aaacatctgc aatttcagat agaattttct ttgtattttc atttataagt
    27661 ttttcaaagt tttctttttc atattcaggg tcttttaaga aaaattcaat aagatacgga
    27721 acctccttta acttctttat tttttcctgc tcaagagata tcgctttttt tatatattct
    27781 tccttttctc tttcaacatt gtcataaagt aatcctttct ctttcaaaaa aggtaaggat
    27841 aactggtata ttctatcaat tgatgagttt cttatatata aactattcat ccacaataat
    27901 ttttcagggt caaaaacagc acttgcttta tgacatcttt caattgaaaa ctcatttatt
    27961 aactcttctt ttgtaaaaat ttctttactt tcagttgttc cccatccaag taaagaaata
    28021 taattaaaaa atgcttctgg aagataaccc tcttccttat atgctcttac agatgttgct
    28081 ccatgtcttt tgcttaacct tgttctatca ggtcccaatg tcatagacat atgggcaaat
    28141 tgagggattt ctgcttttat tgctttaaaa attaaaacat gttttggagt attgggaaga
    28201 tggtcttctc cccttattac atgagttatc ctcatcaaat aatcatctat aacaacagca
    28261 aaattaaaag taggggtccc gtctgacttc attataacaa aatcggttaa atgttctcct
    28321 tcaaaaacaa ctctacctct tattaggtca tttatctcta ttttctcttc aggaactttt
    28381 aaccttaaaa catactgcct tccttctctt tcatactgac ttattttttc ttttgttaaa
    28441 ttcctgcatc taccatcata accaggagga agtcctttct cctgtgcctg ttttctcatt
    28501 tcttcaagtt cttcttttgt acagaaacat ttataaacaa acccttcctt taaaagtttt
    28561 tcagcaaatt ccctgtaaat ataaatcctt tcactctgcc tgtaaggacc atattctcct
    28621 ccaacatcag ggccttcatc ccattcaaga ccgagccact ttaaatcttc tattatcgcc
    28681 tcttcatatt cctttctact tctttctatg tctgtatctt caattcttaa aacaaatttt
    28741 cctttattct ttcttgcaaa taaataatta aataaagcag ttcttgcatt accaatatgt
    28801 aaaaaacctg ttggacttgg tgcaaatctg actcttacca tatctctcct tttttaaaaa
    28861 ttatacttca aaaccataaa aaaataaaaa gtacctgtct ttcttaaaaa tgaacaaaaa
    28921 atttaattat tttcacgcat aatttaatcc ataagatggt agagggatgg gtaaaatttt
    28981 tttccagata ccattttgat tttcttcatg gaagttaaat aaaaattcag aaaaagttgt
    29041 taaattctca aatttttttt cttcattata cgcttttaaa agaaatttgt tatcttctgt
    29101 cctgtattca atctctatat tcctttctct gaaaaaatca tataaagaac tgaaaagaat
    29161 ttcagggtta ataagtaaaa tcgttcccat ctcccttttt tccttttctt ttccaagaag
    29221 atagtgtaat tctctatccc ataaaggaac aaatatattc tttttccctc ttctactttt
    29281 ccatgcttca acaagaaact ttcttgaacc tgcaaattct ctgttgtatc caatatttat
    29341 aactcccttt ctctctcctt ctgcccaata acctaaaatt tcccgttctt tctgaataac
    29401 atatattctg ctttcgcata acattacttt ttttctatat tttccgttat ataaagaaaa
    29461 aagttgtgag aaatctttgt ttcttataaa tctaacaggt tccttttgat aaattttcat
    29521 acaatcagaa aacatttcat ccctgaacct ttttatttta aaatctactt ttttattttt
    29581 acctctaatt tgataaacat aataaagcat acttacagca cctatccttt tatataaaga
    29641 cctgcctcct gaaatcatta aatatgctat tttatcttct gttgcctttt ctattgcttt
    29701 ctgtaaaaga gcagaggcaa atcctttacc tctataatct ggatgtgtac agacatttcc
    29761 aatttctcct attttcattc tattaccgta aacatttatt tcttcttcca ccagcccgac
    29821 aaaagaaaca atttttttat tctcaattgc tataaaagta tttcctttct ctatattctc
    29881 tttgctgtac attatcggtg tctcaatacc catgcttttt tctttaggac ggaaaactaa
    29941 atcagccaat tcaatcattt catccagaac ttctaaatta ccctgtctta tctccatttt
    30001 tatcctcctt ttattcagta agtatagatg atgcaagata aagagaacca aaaattaacc
    30061 agttttcttt tgtccctttt atgtatttat atgcttcttc tggattttca attattttga
    30121 catttttttt gttttttaac aaacctttta aaacagatgg ctcaatggct ctatctgaat
    30181 ttggctttgt taaaattatt ttacctgcgt gtttttcaaa aatcttaacc atgtttttgt
    30241 agtccttatc ctttaaaatg ccaacaagat aattaaatct agtatcagga aatattttct
    30301 tcacattttt catcaaattc ttaactccgt caacattgtg tgctccgtca aaaataatat
    30361 aagggttttt ttgaagaatt tgaaatcttg ccttccagtt tatgtttttt attgcctctc
    30421 ttatatcttt ctcttttatc atgtatccct tttccttcaa aattaaacct gcctcaatcg
    30481 ctattgacag attttctacc tgatgagttc ccaatagatc tgtttttaaa tttttatatt
    30541 tctctcttcc attaaaatca aaaatcatcc ctgaaggaga aagagaaaca attctgcctt
    30601 taaaatcttc accataaata aataattctc cttttttatc ctttaccttt ttctttataa
    30661 catcaattgc atcttttttc tgttttgctg aaataaccat tgttttttct tttattattc
    30721 ctgctttttc atttgcaatc attttataag tttttccaag aaattctgta tggtcaagtc
    30781 caattttggt tattatctct aaaaaagagg gaaggacatt tgttgcgtca aaccttcctc
    30841 ccattccaac ctcacataca agaatatcaa tttttttatc ataaaaataa gaaaatgcta
    30901 ttattgtaag tgcttcaaaa tatgtcggat aaaggtgata tggctgtttt tctataagtt
    30961 ttcttaactg ttcaattttc tctataaaat ctttctcttt tattttctct tcatttattc
    31021 ttattctttc ttttacatca atcaaatgag ggcttgtata caatccaacc cggtatcctg
    31081 aagtttgaag aattttgctt aatgttttac atacagaacc ttttccattt gttcctgcaa
    31141 caagtactga tggatattta tagtgtgggt taccgagttt ttcaagataa taaatggttt
    31201 tatcaagtcc aagtttaatt ccaaaatcac ttaaagaatc aagaaattct aaaggtgtga
    31261 gattgttcat gaatggaaga ttcttaaaat ttttattaat gtttctttta aatttttcct
    31321 ctctacaacc atatcaatta aaccatgttc atataaaaat tcagatgtct gaaaacctgg
    31381 aggaagtttt tgtttaatcg tctgctctat aactcttggt cctgcaaaac ctataagtgc
    31441 ttttggttca gcaattatta tatcaccgag aaatgcgaaa cttgcagcaa ctcctcccat
    31501 tgttgggtct gttaaaatag aaacatacag aatacccgcc tttttcaatt taccaacagc
    31561 tgctgaagtt tttgccattt gcattagaga aacaacgcct tcatacattc ttgctccgcc
    31621 accccctcca gaaacagaaa taagagacag tccattttca accgcaaatt cacataacct
    31681 tgtaaatttc tctcccacaa cagaccccat acttcccata ataaaatgag cgtcaagtac
    31741 acagaaagcg acagggaagt ttccaatttt tcctgttcct gttaccattg cttcattcat
    31801 tcctgtattt ttcatttctt cttcaagttt ttccttatat gatttaactc ctacaaaatt
    31861 aagtgggtct tccgactgta aatctcccca tttttctgaa aaagtacccg catctataag
    31921 agaattgatt ctttcttttg cggtcattct gaaataaaag ttgcattctg gacatatttt
    31981 taaattctct tcaagttttt ttgtataaat taattttcca catttttcac atttgaccca
    32041 caattctttt ttaatttcaa ctttttcttt cggtgaaatc tcaatatacc gctttctttt
    32101 gaatatcata aaactccact ctcctttaat gttttcttaa tcttttctat actctcatca
    32161 ctcggtccac ataaaggaag tctccattca ggacttatca tacccattaa agaaagacat
    32221 gtttttacag gtattggatt tgtttctata aaaagaattt taaagattgg gaaaagttta
    32281 agatgtatct tccttgcatt ttcatagtca ccatttaacg tatattcaac catctgagaa
    32341 acttcaagag gaactatatt tgaagcgact gaaacaaccc cttttcctcc aacagacata
    32401 attggtaaag ttaaagaatc atctccggag agaactgtaa aatcttctct ttttaatagc
    32461 gtaagtattt tacttacctg atcaagagaa ccacttgctt cctttatccc aactatattt
    32521 tttatttcgg aaagttccgc aactgtttca ggtgtaatag aaatccctgt tcttgaaggc
    32581 acattgtata gaataagagg tattgatact gcctttgcaa tcgccttata atgaagataa
    32641 agtcctttct gagttggttt attataataa ggggttatca aaagtgctgc atctgctcct
    32701 accttttcag catattcagt aagttcaatc gcttctttag tattatttga accagtacct
    32761 gctataacag gaactttctt attaacatat tcaacagtaa actttatcaa atctttatgc
    32821 tcttcataac ttaaagttgc cggttctcct gtacaaccag caactaaaat cccattggtt
    32881 ttattcttta tttgaaaatc aataagtgtt ttataggaat tataatcaac cttatcattc
    32941 ttaattggtg ttacaagtgc aacaattgct cctttaaaca tatttcctcc tttttcaaat
    33001 taagaatttt atatcaatat aattaaaacc aaattctttc aacttttcaa ttattttttt
    33061 cttattcatt ctacaaagag aaagaaaaca actattttca actttaatat aaagtttttt
    33121 ttctttttcg taaagaacat aacttttccc tctcattttt tcatctatta tccttggcca
    33181 gagaacagtt atatcaatat ttctattctt ttcgtcaggt atctcaattt tatttaaaac
    33241 ccttttaaca atactatcta ttttttctgt tttcatttct cattctgttt ttaatggcat
    33301 acaaacatat aagaaattat cttcttcctc tggtttcatt aaaactggtt ttgaagaaga
    33361 tgtaaaagaa aaaataaact cttttttttc tactacttgt aaaaaatcaa tcaaaaaatt
    33421 aggaggaaaa attatatcta tttcctcatc actgtaagga atatccattt tttcctgagc
    33481 ttctccaagt tctcctgaag cggataaaat taaagcatct tttttaaaac ttaatttaac
    33541 tttattattt ctttctgaag taaaaagtga aattctcttt aaagacgata aaaataaatc
    33601 tgtattgatt tgagaaattt ttgatttttt aatatcagga ataacttttt cataatcagg
    33661 aaactcttct gctcctgtaa gtagttgaga aataaacaaa acacttccta ttcctttttt
    33721 gttttcttcc atctgtattg taaaacttgt ttgaaaagat atattatttt ttccaataaa
    33781 aacttctact tcttcattat ttaataaatt tatcattttt tttattaaat tatatgataa
    33841 taaaaccttt gtatttttct taaagggagt atcaaataaa atagagaaaa gagataatct
    33901 tctggtatct gttcctatta tatttataac tttttccttt atatcaaaaa gtcctcctct
    33961 gaactgctgt cttggttcat ctgggtcaat acagaaaata gtttttttaa aaccatcttt
    34021 taatgtttct cctttaattc tgaaagttat ttctgaagag aattttggga ttttaggaaa
    34081 atcttctgtt tccatttcta taaatgaata ctgggatgat tctcctatta aattaataaa
    34141 cttttctttt ttttctattt ttatttcttt atttttaaat tgttttaaga gagagataaa
    34201 ttgttttcct ggaataacac attgaccttt ttctttaaca tcacattcta aagaaacttt
    34261 aacagtattt tcaaggtctg ttgcaattaa cgttaaatta ttttcttttc cttctaaaaa
    34321 aattcctcca caaatcgcta ttgttggttt cgcaggtaaa acatctgaaa tgtactcaag
    34381 ccctttattt aaaacttcac tacttatatt tactttcatt tttaatcctc ctttttatat
    34441 atattttata attaatagta aaattgtgga aaaatactaa aaaatttaaa aatctataat
    34501 ttgtaaataa atttttatgt gcaaaacctg tgaattcact gttcatttaa tttttccttt
    34561 atattttcta ttattttttt taaattttta tccttttcca ttaactcttt aactttttta
    34621 caggcatgta aaacagtcgt atggtccttt cctccaaact cagaagcaat tgaatttaaa
    34681 gaagtttctg ttaattccct tccaagatac atagctattt gtctaggaag aattatattt
    34741 ttaacccttt tttcactttt tatttcttca ggagataaac caaaatactc acaaacaaca
    34801 ttcattattt gtttaatatt tacaaacttt ttttcttttt tatctgaact gtatacaccc
    34861 tctattattt catctattaa gtttttatta agttctctat tcagtaaggt cacatttgca
    34921 acaagtccat ttaaaattcc ttctattaat ctaatattat cttttatttt ttcagcgata
    34981 taaaaaataa ggtcttcaga gaaagaaagt ttcctcaatt cacttttttt ctttattatt
    35041 gcaacccttg tttctatatc cggggtctgt aaatcaacta taagacccca ttcaaacctt
    35101 gatacaagac gtttttcaag aaactttatt tcttttggcg gtttatcact tgaaagaact
    35161 atctgttttc ttttatcata gagaaaatta aaagtatgga aaaattcttc ctgagttcct
    35221 tcttttcctg caataaaatg aatatcatct attaaaagat aatcaagatt acggaattta
    35281 tttctgaaaa gatgagttgt tttatttttt atacttgtta taaactcatt tacaaaatat
    35341 tcagatggca tgtaagcaat tttagcatta cttttctcct taacaaaatg accgattgcc
    35401 tgcattaaat gagttttgcc aagtccaaca cctccatata gaaaaagagg gttataagca
    35461 actccaggag attgagcaac agcaacactt gcagcatgag caagacgatt acatggacca
    35521 acaacaaagt tttcaaaagt atactcggga ttcaatgcta tttcaaaaaa actttcttct
    35581 tttatttctg aagggaggtc tatagaataa attatttcaa aattaggatt aaaaccaaag
    35641 attttttgta tttcttctct taaaatttct atataaggta aaaaattctt ttcaaaagtt
    35701 gaatttggaa cttctatttt tacaatattt tcagttatat ctataagttt tacaggtttt
    35761 atccataaat caaaggtcct tgaattattt atcttttctt ccacattatt gagtacgata
    35821 ttccacagtt tatgaacttc tttttccatg ttttccacag gtttttaaca aataaacttt
    35881 tgtattattc attttttcca caacaatgtt tatatttttt accacttcca catggacatg
    35941 gttgatttct tcctattttt tgttttttca aagaagcggt tggactgtta attacatttt
    36001 tattatataa accttcagaa atactctgtg caacagaaac aggttcttct ttttcttttg
    36061 ttgtagcaaa ctgttcatat gtttgatgga caaactcctt tctttctgtt tctctaactt
    36121 taagagcaac cttttctgta acctgaattt ttaataaatt accaataatt tcttctttaa
    36181 tagattttaa catttcctga aaatattgaa aagattcatg tttatatgct attaatgggt
    36241 ctttctgagc atatgctctt aaacttatac cttctctcaa ttcatccatt gcccttaaat
    36301 gtgctttcca tctattgtct attacctgta aaaacacaaa tttctttaat tcttcaaaaa
    36361 attcacccag ttgtttcttt ttatcctcta atacagaaat gaagatatcc tttaactttt
    36421 ctttaaaatc atctttccac aaaaggtaat tttttatatt ttcaggattt tctatttcaa
    36481 tatttattcc aaaagttgtt ttaaaatatt tagttaatcc ttctatgttc cacatatatg
    36541 gttttatttt ttcatcacag tattcattga aaactctctc aataatttct tctatatact
    36601 cttttaaata ttcatcaaga tttctatttt caagaatgtc ctgtctcaaa gtgtaaataa
    36661 tatttctctg ttcattcaaa acattatcaa attcaagaag attttttcta atttcaaaat
    36721 tccttccctc aacttttttt tgtgcattat ttatcatttt tgttatcagt ggatgagtta
    36781 ttttttctcc ttcaggtaac tttcccatca atccttttaa tctttcccct ccaaaaattc
    36841 tcaaaaggtc atcttcaagg gataaaaaga attttgaaga accggggtct ccctgccttc
    36901 ctgctctacc ttttaattga ttatctattc ttcttgcttc atatctttca ctcccgatta
    36961 catgaagtcc tcccagttcc actacttttt tatggtcttc ttcccacggt tttttgtatt
    37021 tttcaagtaa ttcctgatat atttccttaa attcttccgg acttttttcc atatcttctc
    37081 taattttttt ccattcttct tcatactgac ttctttctct ttcatattcc tctcttgcaa
    37141 gattcaattc ctcaatatat tttttctgaa ttttttcata ttctctaact gttttttcat
    37201 attctttttc aatttctaaa taatctggag aatatttttt ataaatttct ctttcaattt
    37261 ctgtaaaacc ctcttctaaa cccttgtaca catgatattc tctattttta aaatcagaaa
    37321 caatctgttc aatttgagtt ttttccgata tctgattttt caaattatca ataaattcaa
    37381 tgtatctatt tatttttttc ttactttttt ctgcttcttc tgtaaaaaga atttcagggt
    37441 atacttcttc aagagttttt atacaggaaa tacatgtatt ttttattact tccaacaaag
    37501 taatcaaaga atttattaat tccttttcat ttttctcatt ttcaataatt tctataagac
    37561 ggctcctctg ttcttctata ttttcttttg tttttatatc aaagaggttc tttatgtaat
    37621 ttttgtaatt tatatattcc ctgaatgctt ttcctgttgt ttcccttatt tcttttgttt
    37681 tttctggttt atccttaaac tgaatattca attcctgtaa tttattatta tttgcttcat
    37741 aaattgcttt caattttaaa agtttatttt catacctttt aaacaatcct ttagattttt
    37801 tttcaaatat ttctttttca aatatttctt ttactacttt ttctttctga tataaatttt
    37861 tttctatctc tgataatttt tctttgaatt ctttaagttg tgttcctata gttttttcaa
    37921 tttcttcctg cctttttttg tatttttctt ctatctcgtt tactatatca ataaaatctg
    37981 actttatatg tttcttttct cttttccttg cccataaaat tttaaccgtt tcttctcttg
    38041 caataatttc cgggtttcct ccaagaataa tatcaactcc cctacctgcc atctgagttg
    38101 caatagttat tgcttttggt tttcctgcct gtgctataat tgctgcttct gcttcgtggt
    38161 ttttaccgtg taatactttg tgaggaatgt tttttctatc aagcatttta cttaactttt
    38221 ctgctttttc tatagatatt gtcccaacaa gaactggtct tcctatttta taaagttctt
    38281 ctatttcctt caatattgca ttgaacttct ctttttctgt tgaataaatt tcatcatcat
    38341 attcttttct gtttaatggt ttatttgttg gtattaccac cacatcaagt ttataaatct
    38401 ctttaaattc aagtgcttct gttattgctg ttcctgtcat tcctgcgatt tttttataaa
    38461 gtttaaaata attctgaaaa gaaattgttg caagtgtctg gttttcactt tcaattctaa
    38521 ccccttcctt tgcttcaatt gcctgatgaa gtccatctga ccatcttctt cc
//
LOCUS       DAOSAT010000004        38244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_800_length_38244_cov_5.032156, whole genome shotgun sequence.
ACCESSION   DAOSAT010000004 DAOSAT010000000
VERSION     DAOSAT010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 38244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38244
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_800_length_38244_cov_5.032156"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLF90_00605"
     CDS             <1..553
                     /locus_tag="PLF90_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK55816.1"
                     /translation="LIVDNFKLVEAVFNRVGELIYEFYKNVIEIDNVEGFFQGDDLGF
                     KTSTFLSPEMLKKLVIQWHKKFAEIAHMRGKMYWFHCCGNILNVIDDFIHDVKIDAFH
                     SFQDEIIPVWEFKEKYGNKIAVLGGVDVNKLATYDEKNLRKYVREILDRCMPYRYALG
                     SGNSITNYIPPENYLIMLDEGLR"
     gene            complement(871..1965)
                     /locus_tag="PLF90_00610"
     CDS             complement(871..1965)
                     /locus_tag="PLF90_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK55817.1"
                     /translation="MKIKFPEPDLNDFKKVLLREVKGSRIHCAELHIDKEILKFLLED
                     YFGIKWIEPIDYETQKKAIQNVVNLYYKLGFDTIRLSSDFRFTSNLKFEIPKRETDDT
                     SLFSKGKRKWAEEKEGVIKNWNDFENYTWPEPEKVDIWVYEYLSEILPENMGIFLCIS
                     QGIFETLMFLFGFENLCYLIYDNEEIVKKTSEKIGEIIYKATKKFIEKEKIIGIFQGD
                     DMGFKTQMLLPPEFFKKYILPWHKKLSELAHQNGKIYVLHSCGNIEPIMDDLINEVEI
                     DAKHSFEDEILPVWKFKEKYGEKIGIIGGVDIGKLCTLKENDLRKYIRKILEKCAGSG
                     YILGSGNSIANYIPPENFIIMLEEGYSFKI"
     gene            2012..2404
                     /locus_tag="PLF90_00615"
     CDS             2012..2404
                     /locus_tag="PLF90_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55818.1"
                     /translation="MEKEIKRYRIRPKTYLCLTPEEAITTGISTGEQIIEELILVREF
                     TLSAYMYGVTRNLPEEELKKMKVEERGEKLFESRKEAINYLKEKGEKFFSLYIVKETR
                     MFPREEIKWDETEKSIEDFLSQLKEFEK"
     gene            2489..3811
                     /locus_tag="PLF90_00620"
     CDS             2489..3811
                     /locus_tag="PLF90_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOK55819.1"
                     /translation="MIEKYFKFRERGTDLKTEIIAGITTFITMAYIIFVQPAVLSQGG
                     MDFGAVMVATCFSSAIATLIMGLYANYPIALAPGMGENFYFVYTVILGMGIAWQKVLG
                     AVFISGILFIILSLWKVREKLVENVPSSLQCGIAGGIGLFIALIGFIQSGIVQKGGVV
                     LTMGNIYQKEVILSIIGLIIMGTLMALKVKGNILIGMIITAIIGICSGIIKYEGIIGK
                     IPSIAPTFMKLDVIGALREGIFTVIFVFLFMDLFDTVGTAIGVGEAGGFIKNGKFPKV
                     ERVLLSDAVGTVVGAIFGTSTVTSYIESASGISVGGRTGFASVITAVLFLLSLFFYPF
                     VKMIGTGIQTVGGITLYPVTAPALIIVGSLIIPTIRKIKWDDPTESIPAFLSFVGIPF
                     TYNIGEGIAFGFISYFLLKLFTGKYRNLNPVVIIVAVAFILRFILLKN"
     gene            3891..4196
                     /locus_tag="PLF90_00625"
     CDS             3891..4196
                     /locus_tag="PLF90_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55820.1"
                     /translation="MTCWFFDRGGNSLKDAVIIYADSFVERIGIIFDYLSLKHGKIGS
                     DWRVFSIQRIRDKDRNYEIWSIEIYKERKIKEIKTYYFEISGVKNSKKTETFFKFLS"
     gene            complement(4214..5506)
                     /locus_tag="PLF90_00630"
     CDS             complement(4214..5506)
                     /locus_tag="PLF90_00630"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOK55821.1"
                     /translation="MKKKSINIGVIGCGTVGSSFIKKLISRKNIIKEKTGIEINILKV
                     FDKDKGKLNQFKDISAESPDEIIENKNIDIVVELIGGTDYAYEFVTKAIKNGKSIVTA
                     NKALLSKYGKELFKKAKEKNVYIGFEGSVAGAIPIIKALKESFTGNKITKILGILNGT
                     TNYILSEMYHKGIDFEKGIEEAKKLGYAEADPTLDIKGYDTLHKILILTYITYGELFS
                     AEEIYVEGIDKIQTIDIKFASDFGYRIKLLGVCRKEKNQIEIRVHPALLPKTHLLSLV
                     EGVNNAVYVEGDLIGKSLFYGEGAGGNAATSSVIGDVVDISKKIVSEGFVKENFVSED
                     IISLKPFEEIETKYYFRFTAIDKPGVLAKIAKILGDNDISILSVIQKQDNPEKAVPIV
                     MLTHKAKEKNVKKAIKEIDKLDVIKNPTVFIRVEEDLM"
     gene            complement(5510..6241)
                     /locus_tag="PLF90_00635"
     CDS             complement(5510..6241)
                     /locus_tag="PLF90_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55822.1"
                     /translation="MKKLLKKIKKIVMVLLIFGILGGGWFYYNKYKTEKLRKLVEEEK
                     RRQEELEKEKQRKLLEEKQKEFENLLTEMEKYFKAGDYIKVKEISEKALALAKQFNFS
                     TDRINEIFHKIEINTYLSKLKKLERENEDIYKYFYVRIEVKKIPPVKEVLNLRNKILN
                     KTYENEYKVKLILADKALDELKKGIEPSYNYFLSRKFYAEAKKLRETRNIRKEELENK
                     VQSIQNELFFASKSLYKNTIPNSLY"
     gene            complement(6319..7107)
                     /gene="trpA"
                     /locus_tag="PLF90_00640"
     CDS             complement(6319..7107)
                     /gene="trpA"
                     /locus_tag="PLF90_00640"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOK55823.1"
                     /translation="MTQIEKKFKELKIKKEKALILYFTAGFPSFENSIEIIKKSAEKG
                     ADIIEIGIPFSDPIADGPVIQYTSAKALENGMNTNKCFELAKILKNEMNIPYLFMTYF
                     NPVYRYGIEKFADRCVECGVSGLIIPDLPYEESFEIKEILKKREMNLIYFLTPFTSEK
                     RMKKITRMANGFIYFISVAGVTGQRDSLNPEIIDVIKKVKSLKEIPVALGFGISTKEQ
                     IKQIKEYVDGIIIGSFFLQKIIDGKIDEVEELIYQFKKELKNVF"
     gene            complement(7104..8285)
                     /gene="trpB"
                     /locus_tag="PLF90_00645"
     CDS             complement(7104..8285)
                     /gene="trpB"
                     /locus_tag="PLF90_00645"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOK55824.1"
                     /translation="MLPDRKGKFGIFGGRFAPETLMGPLEELETAYKKISKTKEFKEK
                     LNYLLKEYAGRPTPLYYAENFSKYLGFKVYLKREDLAFTGAHKINNTLGQVLLAKMMG
                     KKRIIAETGAGQHGVATATASALLNMGCVVYMGEIDVERQKLNVFRMELLGTKVVPVS
                     SGSKTLKDAINEAMRDWITNFKTTHYVIGSVVGPHPYPMIVRDFQSVIGKETKKQILK
                     KEKKLPDYLVACVGGGSNSLGLFFPFYRNKEVKFIGVEAGGKGINTDKHSATLLKGEK
                     GILHGSFSYVLQDNYGQIKTTHSISAGLDYPGVGPEHAFYKETGRAEYYAVDDKTALN
                     GFHILSELEGIIPALESSHAIGWLIENKNRFKEKDIVVVCLSGRGDKDVMEVAKIEGD
                     L"
     gene            complement(8275..8913)
                     /locus_tag="PLF90_00650"
     CDS             complement(8275..8913)
                     /locus_tag="PLF90_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOK55825.1"
                     /translation="MIEIKICGFKRRKDIIDAVNLGIEWIGINLYEKSPRFVEEREAK
                     LLIENLPSNIHKIGLFVNPDEKNLFETVKKLKIDGIQLHGNEPPSLLEKLKKNFPDKI
                     IIKAIRVKNYEDIVKRMKHYKMADYFLLDRYEEDSFGGTGKMIDKEKIEKGKLPWNKI
                     FIAGGITPENVKNIIKKYRPFGIDVASGVEIAPGIKDIEKIKKLIEEVKNVT"
     gene            complement(8913..10256)
                     /locus_tag="PLF90_00655"
     CDS             complement(8913..10256)
                     /locus_tag="PLF90_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOK55826.1"
                     /translation="MEENKIYLSERELPQAWYNILPDLPEPLPPVIHPKTKEPVKPED
                     LAPIFPMSLIMQEFAPERWIEIPNEVLNAYKIYRPTPLHRARNFEKALKTKARIYFKN
                     ESVSPPGSHKPNTAIAQAYYNKIEGVKRLTTETGAGQWGSALAFACKLFDLECVIFMV
                     KVSYYQKPYRRLLAQSWGATMYASPSDKTEFGRKILSQNPNSPGSLGIAISEAIEEAV
                     KREDTKYSLGSVLNHVLLHQTIIGLETKKQLQLIGEKPDVLIGCVGGGSNFGGFIIPF
                     VPDKIKGENIRFIAVEPKACPTLTKGLYRYDFGDTACTTPLLKMYTLGHSFVPPGIHA
                     GGLRYHGDAPLLCHLYKLGLVEAVAYHQRECFEAGVLFAQTEGFLPAPETTHAIRAVI
                     DEAKDAPEGKVIVYNHSGHGHFDLSAYEAYFEGKLEDYEYPEEKIKEALKDLPEI"
     gene            complement(10276..11061)
                     /gene="trpC"
                     /locus_tag="PLF90_00660"
     CDS             complement(10276..11061)
                     /gene="trpC"
                     /locus_tag="PLF90_00660"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOK55827.1"
                     /translation="MSDLISEIIEKKSIIIEEKKKILPTEKLIGMISLKKKYSIYQSL
                     KNEFGIIAELKRASPSAGTINNNLDFKSTAIKYQKSGVSGISVLTCEPYFLGSIDDLK
                     TVKESVEIPVLMKDFIVDEYQIYEGKYYGADFILLIVRILEDRKLEKFIKICEETNIE
                     ILIEIFDINDWKRVSNLIKRWKDKILGINNRDLKTLKVDINNTLNLIKHIPVDKIIVI
                     SESGIKEKEEVLLLKNLGLKGVLIGESILKSKNVEEKIKELKN"
     gene            complement(11054..11674)
                     /locus_tag="PLF90_00665"
     CDS             complement(11054..11674)
                     /locus_tag="PLF90_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55828.1"
                     /translation="MKKILILFLFCFGFAISGENIEIELKKQATVDLCNFPDLTDGIV
                     DILYLTVDNYWHKGEYSKIFPVFYLITRIFPDDVNAYCLGGWFLINGIAPKYEGEKKE
                     EIKKFAIEFMKKGIEKKPDDYRLYWEIAWFYYNENDFENSLKYLEKAENYNHPFYIEN
                     LKAHIYMKQKNLKKAIEEWKIIKEKYPERKEIAEKFIKQIEKEKNE"
     gene            complement(11671..12675)
                     /gene="trpD"
                     /locus_tag="PLF90_00670"
     CDS             complement(11671..12675)
                     /gene="trpD"
                     /locus_tag="PLF90_00670"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOK55829.1"
                     /translation="MEKIFNKLFENINLTTNETYEIFNKIMEGELSSVKTAAFLTLLK
                     IKKESSEEITGAAKLLRDKMLKIRHKKEIICDTCGTGGDYSGTFNISTVCAIVASSMG
                     LTVAKHGNRSITSSCGSADVLEYLGYKIDIEPEKSEILLEKFGFAFLFAPLYHKAMKN
                     VAEVRKELGFKTIFNIIGPLCNPALTNYQIMGVNDYSLLKIIPYVFKNLGIKGYVFHS
                     EDGLDEITLTGKTYIVEIDEEISEFEIFPEDFNLKRCKIEDIKGGNLKENAEILFNII
                     RGKEKGPKKDIVCLNTAFLLKTTGIVDRIEKGIELTDEILKKGIPYRKFNEILEYIKK
                     "
     gene            12924..13142
                     /locus_tag="PLF90_00675"
     CDS             12924..13142
                     /locus_tag="PLF90_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOK55830.1"
                     /translation="MKKLVEAKEGKYKVVDVLKHGKLKKLCNMGIFVGDIIEVVKPAP
                     GPVIIKKGNITIGIGCGIAMDIIVEKVE"
     gene            13145..15139
                     /gene="feoB"
                     /locus_tag="PLF90_00680"
     CDS             13145..15139
                     /gene="feoB"
                     /locus_tag="PLF90_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOK55831.1"
                     /translation="MEEKIVAIAGNPNTGKTTIFNSLTGTRQQVGNWPGVTVEKKEGY
                     YIYKDVKFKVVDLPGTYTLTGESEDQKIAIDFITQNEVDVLIIMADALNLERSLYLLL
                     LLSEFHKNIIIDLNMVDLAEKEGIEYEIQHLEKLLKVKFVKTVGNKNIGIEDLKEEIY
                     KKCKEPPSEFFEFNYGEEIEKVISEIVCQFKNLKLPYPPRFLAIKILEKDEYFTNFII
                     TKVDWKKINDLIMNTEKFLNKPLDRVLMEKRYGIIHGIVKESLIEKKKELKPEITEKV
                     DRILTHNFLGPLFFLFIIFIIFNAVFVWGKPFVNFIELILEKLKIVFSYLNLPIWINS
                     LIKDGIISGVGSVILFLPNIFLFFFFFAILEDTGYMARAAIVADRIMHKVGLHGKSAI
                     PLILGFGCNVPAIMGTRILENKRDKILTSLSIPFMSCSARLPVYLLFTGIFFKENQGM
                     IVFSLYLLGIGIGVLSIKIFGSIFLKDESLPLIIELPPFRMPYWKNVLIESWTRTKYF
                     LKKAGTIIFLGTLILWVLNYFPHPYNYGTEMNFIGKLGSFIFPLFKFSGFGFWQAGVA
                     LIFGIYAKELVVVTFGTLFGVENLTETLRFYFTPISAYSFMLMSLLYMPCLATFLILK
                     KEVGIKWALISLFWSLFAGWFFSTLFYQIFSFLKGGTFVN"
     gene            15129..15542
                     /locus_tag="PLF90_00685"
     CDS             15129..15542
                     /locus_tag="PLF90_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOK55832.1"
                     /translation="MLTKSLEDYLEAIKILTIDKKVTRVKEISELLDVKKSSVVNALN
                     ILKDKGYIKQEKYGYIELTESGDREATKILNKHKIVMKFLTKILNVSDSIAENDACKI
                     EHFISEETLKKMIVLLGKKGNHLNLFKKQGRINKD"
     gene            complement(15497..16333)
                     /gene="speE"
                     /locus_tag="PLF90_00690"
     CDS             complement(15497..16333)
                     /gene="speE"
                     /locus_tag="PLF90_00690"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010884319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOK55833.1"
                     /translation="MQKNRNIIIDWYHPYGVGIAFEIKKKLLEKRTKYQKIEVYETKN
                     FGKVLIIDNLIQSVEKGEESYHEILVHPAMFSHPEPKNVLIIGGGEGATLREVLKHPV
                     KEVKMVDIDEEMIKIAKKYLKFDKGAFEDKRAEIIIEDGFKYIKENNKKYDVIIVDAT
                     DPGEIPSCPLYTVEFFKLCYKHLNKNGIYVTQGGTSIFLHPERIKTVYSDLKKVFKNV
                     KIYSSPVFGLFPNWIFIIAIKGNIKIEKKPNKKIKENLFFYTPELHQSLFILPCFLNK
                     FK"
     gene            complement(16320..16700)
                     /gene="speD"
                     /locus_tag="PLF90_00695"
     CDS             complement(16320..16700)
                     /gene="speD"
                     /locus_tag="PLF90_00695"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOK55834.1"
                     /translation="MRKFQTFVPPNGVHIILDLYECSDFNILNSPEKIKKILLKAVEK
                     SKATLIDVKIHKFSPVGVSGFALISESHISIHTWPEKKYAGVDIYTCGEKTFPEKASN
                     LFIKELKSKKPSIIKIERGIYAKK"
     gene            16894..17565
                     /locus_tag="PLF90_00700"
     CDS             16894..17565
                     /locus_tag="PLF90_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOK55835.1"
                     /translation="MNELEFIKKVVISGILSGIIGFEREKHGREAGLRTMILVGMGTT
                     TILITSFQVYYIFSNFQNSVLRIDPARIAYGVVTGMGFLGAGAIIKDSKRIRGLTTAA
                     CLWFVTSVGLAVGVDLYILACFLTFISLIILYFLKIFEKKVPVDTYSTLTIIGEDKRE
                     LKDKIEKILKNYGFKILSTEIEKNKVKAKIKLTYTIRYKSNQNVNELFEKISEIEELK
                     NIKLN"
     gene            complement(17562..18017)
                     /locus_tag="PLF90_00705"
     CDS             complement(17562..18017)
                     /locus_tag="PLF90_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOK55836.1"
                     /translation="MKEIFITLGLAMLPISELRGAIPYGLSKSLPFFNVFLISILGNL
                     LPVIPLYFFLERILKFLEKFNYGKKFNTWLINRTKSKSEIIEIYKTIGLLIFVGIPLP
                     MTGAWTGTIASVIFQLKFKNFLIGIIGGVLMAGIIVSFVTLGVKNFFTF"
     gene            complement(18014..18661)
                     /locus_tag="PLF90_00710"
     CDS             complement(18014..18661)
                     /locus_tag="PLF90_00710"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOK55837.1"
                     /translation="MKKDFENLRREMVETQIVKRGISDKKVIDAFLKVPREEFVPENM
                     KEYAYEDSPLPIGEGQTISQPYIVALMSEALKLKEKDKVLEIGTGSGYQAAILAEIGC
                     EVYSVERIEALAKRAEKTLKKLGYNVKIKVGDGTLGWEEFSPYDKIIVTAASPEIPDT
                     LLEQLSEKEGRLIIPVGDKYIQDLILVVKTKDKIEKINLGGCQFVPLIGNKGWNE"
     gene            complement(18645..19343)
                     /locus_tag="PLF90_00715"
     CDS             complement(18645..19343)
                     /locus_tag="PLF90_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK55838.1"
                     /translation="MIEDILVIIPAYNEEKAIGKILKELKKYFKNILVIDDGSIDRTK
                     EIVQKEKVLLISHITNKGKGEALKTGFKYAIEKNFPAVITMDGDGQHLPEETLKFVEE
                     YRKKTKIGILIGKRSIFSQSMPLIRRITNLSMSFLISILSFQWIPDTQCGFRLIKTDT
                     LKKIKLYTSHFETESEILIKAGCKGIRIKSLPIKTIYRKEKSKIRPIRDTVRFFKMFF
                     LIFFTSFKKYEKRF"
     gene            complement(19340..21340)
                     /gene="recG"
                     /locus_tag="PLF90_00720"
     CDS             complement(19340..21340)
                     /gene="recG"
                     /locus_tag="PLF90_00720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOK55839.1"
                     /translation="MKISLNSPVNQIKGIGKQKEIIFEKLGIKKVEDILFYFPRKYIN
                     LKKITKIKNLKEDEIYTVKATVLAKEEKRIYGQTNYLKIAVPDGTGILYIIFFNQIYL
                     KNIFQNGKNFIFYGKVEYFNKEFEMINPFYEEYDGRKINWILPVYPLTKGITQKYMRK
                     IIKWVLNNINEFPEEILPLERRLLFGISNIKHALLNIHFPISEINLEKARNYLIFREF
                     FIFQLNLLWKKNFEQKTEERINIELNLIEEFEKYIPFKLTKNQKEIMEEIIDDIKKGK
                     LIRRLIYGEVGSGKTTIAIFTLWIFAKIGFQGVFLCPTEILAQQHYINWNGFFLNQNI
                     SVSLLTGSLKEKEKEKIREEIETGKINVIIGTHAVLSEKINFKNLKVVVVDEQHKFGV
                     KQQEVLKEKSENIHYISMSATPIPRTVALTLYGNMDFSILGEIPKGKRQVITYLFSKK
                     DREKIYSFIKFQVSQNKIGFVVTPAIKENENVESAEEKYIEICENLPEVKSGLLHGKI
                     EKKKQEEILEKFKNKEIKLLVTTTIIESGIDIPSASFIIIEQAERFGLAQLHQLRGRV
                     GRSGEVGYCFLIVYNDDEEINMKLSHFIEKETGFDIAELDFNLRGPGDILGTRQHGIL
                     PLKIGDIEKDIEILKLARKEVERVLKIDPYLEKMHHLKRLIE"
     gene            complement(21337..21882)
                     /locus_tag="PLF90_00725"
     CDS             complement(21337..21882)
                     /locus_tag="PLF90_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55840.1"
                     /translation="MSISALEIVKEFFIINNFYVISKDGFLIVKNPEETGKEIENFVI
                     EKEKIFFINEGIIKVLSWHTLKFTPSVIKKFSAEIFEITEEKFYREVKNFLGKEKFKK
                     ILVIPGLPSTKNLREESIKIMKENGIDHVIFFNTIISGLIDKIDERKVYQSHTLEIIR
                     ILKFYKFFYKKNLDAFLFDEK"
     gene            complement(21886..22662)
                     /locus_tag="PLF90_00730"
     CDS             complement(21886..22662)
                     /locus_tag="PLF90_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOK55841.1"
                     /translation="MGLIIFILGLIFGSFANVCIYRLPKGKSIISPGSFCPNCNNSIK
                     WYDNIPVISYIFLKGKCRYCKKTISPRYLIVELLTGILFFLIYKKFGMSISTLIYAFL
                     TLCLIIVSFIDIDTFLISDVIVIPGILIGLLISFLFPQIHDMEKIKGFIYSLEGTILG
                     GGILIFLALVGKLLFKKDAMGGGDVKLLGMIGSFLGWKSVFLTLFFGSLFGTLISLTL
                     ILLGKRKIDDYVPFGPYLALGALISIFFKGNYFMGIILIN"
     gene            22757..23548
                     /locus_tag="PLF90_00735"
     CDS             22757..23548
                     /locus_tag="PLF90_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55842.1"
                     /translation="MKYVNLKGIKISRFIIGGNPFSGFSHQGIEMDMRMKKYFISQRI
                     KETLREAEQWGINTFIGRADNHILRLLLEYRQEGGKIQWIAQTCPEIKSQELSIDMAI
                     SGNPVGCYIHGGYVDFLFLNNEMGKIYPLIEKIKKYGILSGIAGHISEVFLWAEENID
                     VDFYMCSYYNPVNRKEKPEKDILQEEIFSDNDREKMIMAIKKLKKPVIHYKIMAAGRK
                     NPEEAISFMSKNARENDMVCIGIYQEEKRWMIKENIELMRKFGFL"
     gene            23596..24228
                     /locus_tag="PLF90_00740"
     CDS             23596..24228
                     /locus_tag="PLF90_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOK55843.1"
                     /translation="MENIWISSWKHCDIFGKVDLVILAFLSIYSWYIIFEKLFVLKKA
                     FKRNKDFEKIFLSQKEIKRIPCPLYSVLDYLTSLIKFEKFSPEISKKYIEKAFIKEQN
                     NLEKNLTSLATITAIAPFLGLLGTIWGLLIAFTNMSITGSSSIKVVASGVAEALITTV
                     LGLLVAIPAAVGYNFLREKIQLLMDEMEFLIPYIEEYLNELFLKQNEKQK"
     gene            24212..24604
                     /locus_tag="PLF90_00745"
     CDS             24212..24604
                     /locus_tag="PLF90_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOK55844.1"
                     /translation="MKNRNNKNSLAQINITNLVDIALTLVIILLMIAPMIEQGIEVKL
                     PKASPYEMKIEKSIVITVAPENQYFVGGKKMNLNEIYSFLKEKSQEKNISVIVKGDEK
                     VQYENIIKILDIAKKCKIDMIGLATRTE"
     gene            24655..25341
                     /locus_tag="PLF90_00750"
     CDS             24655..25341
                     /locus_tag="PLF90_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOK55845.1"
                     /translation="MFFIPIPEKNKNFEKEIFVVKLVNIPHIEVIEKTSEKKEIKIEE
                     RVLPPVKKEEKIKTVKDIKVEKKVEFSETFSPEEYKKKLYSKISDTKFSETNKKSSNN
                     TETSNINIKIPEIKSVKISTPSFESTSSYQTSFSTDIPSWYINLIKKKIEENWLLKEY
                     LIDMSTIVSFRIYREGRIDNISIEKSSGYKKFDNSIIDAIKSVKSWPAFPSEIKDRYL
                     DVIIEFKTEG"
     gene            25343..26557
                     /locus_tag="PLF90_00755"
     CDS             25343..26557
                     /locus_tag="PLF90_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55846.1"
                     /translation="MKKVFLFFMILSFYIFSQTQIFFQITKNLVEKADTFLIFKVEKN
                     DFSSKMLETLSQDLKFSGYFNVEGIKFTDDIEKTKKEIVTQIIITGEQKGEIINVKVE
                     DGLEKNLLYEYSYKISKDPRFLAHMICDDIVEKLTGKPGIAKSKILFTSDRTGKKQIF
                     QIDYDGFNLKQLTNVEYLVNYPRYLEGNKIIFVSYEDGWPKLAKMDILSGKIEIFIAH
                     PGLNACASPLKKTREIVLVLSKSGDPEIYLADFDGKIKKRLTENKKIDSSPSFSPDGK
                     SIAFVSDRDGKPQIYIMDRDGYGVRRISYISGYCTSPVFSPDGNFVAYIYSEGSTFGL
                     AIYDINTKKTRTFPGLNCEEISWAPNSRHIVYSKIERKQSLVILDIFTKETRTLISGN
                     FNCFSPNWFLIE"
     gene            26560..27429
                     /locus_tag="PLF90_00760"
     CDS             26560..27429
                     /locus_tag="PLF90_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOK55847.1"
                     /translation="MKGYIFGLISVFSWGTVFVISRFLIHGEVIDPLVLAFWRFFWAS
                     FFLMFFIKFNFKKVFRIFKKDVFRFLILALSGIYLMSTFIFISVKTSSATISSILMSS
                     NPIFVILISILFLKDKITPEKILGVFIGFIGCIIVIKEDFKIFFWAGENINIYSLLAA
                     ICWAVYTVIGEEPTKKYGAIETTFIASFIGTIFLFLTVKLLNLNLLGKNIYGFLSGIY
                     LGIVPTGIGFTLWFSALNYLRPFLLAPFQYLTPIITYFLSILILKEKVSLIVFMGMFL
                     IFFSLFLIQKKEN"
     gene            27488..27799
                     /gene="secG"
                     /locus_tag="PLF90_00765"
     CDS             27488..27799
                     /gene="secG"
                     /locus_tag="PLF90_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOK55848.1"
                     /translation="MYIFLLILHILVSLFLIFAVLIQSGKGSSMANIFGGGGMENVFG
                     AETPAILNKITSFLAVMFIITSVLLAIIPGKLTTKSILKEELNRPGKILPVNPSTPPK
                     N"
     gene            27808..28542
                     /locus_tag="PLF90_00770"
     CDS             27808..28542
                     /locus_tag="PLF90_00770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOK55849.1"
                     /translation="MRKLVELPKKGKLVIIGDTHGDFNSTRIVVKNFIDKKNYYLLFL
                     GDYVDRGPNSKENIDYLLSLKEKKENLILLQGNHEMYPVVECSPSDFWDNLSEDEFNF
                     YKEEFINLPFVAYGNGFIALHGALPDIEKLEDIEKIEIGDKEWFKIVWGDFKDKEGEY
                     LGDFLGRPKFGKDYFLKIMEKIGLNVLIRSHDPYAPERMFDNKCLTVFTSIAYGTERK
                     IALVNLKKEIKSIDDIELISLDKPEV"
     gene            28544..29407
                     /locus_tag="PLF90_00775"
     CDS             28544..29407
                     /locus_tag="PLF90_00775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOK55850.1"
                     /translation="MRIKNFGGYSLGEYLGGGLYTKVYKAYSFLAKPTYGNPVAIKIL
                     YLKGGIVERNKLIKQFEREAEIAMNLEHPNIVKVYNFGRFDHHYAMIMEYIDGKNLKE
                     FLYEKGKWDLNKILRICYEAGKGLEYIHQHNIVHKDVKPDNILISFDFEKIKFTDFGI
                     AKLPKKWWHRDIFPKSGTITKFGIISYIAPEQMKGNATFQSDIYSFGVTMDEIITSKL
                     FMEGKEDEDYFKRIDIRAQRKNTGKQRILCEDMPVPSELKNIIKKATENEINLRYQSM
                     TYLLEDLKKLI"
     gene            29409..31088
                     /locus_tag="PLF90_00780"
     CDS             29409..31088
                     /locus_tag="PLF90_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK55851.1"
                     /translation="MRKISTLIPILLIFTAGCIEKKQIKFRNTLCLSTSSDPKSFNPI
                     IAKETSTTIITGFIFEGLTKIDGITLQVKPLLAEKWEVDKTGKIWRFYLRKDVLWNDG
                     DKFTADDVVFTFNQLIYNEKIPTSSRDVLTVEGKKIEVKKINDYTVEFILPEKFAPFL
                     QLMTQEILPKHKLERSVLEGKFTSTWGVNEKVENIVGTGPYKINDYRPGEWIILEKNP
                     YYWRKDKNGVQLPYIEKIVFLIIPDPNMAILKFRTGEIDILSIRGQDYYVLKPLEKDK
                     NFTIYDIGPSLSSEFLALNQNIDAKIPDYKKEWFSDVNFRKSIAYSIDRNSIIKNVYA
                     GFGTPLYGPMNKSCGFFYNDKITKYDYDLKKARELLMKSGFVWKENFLYDRKGNRVEF
                     TIITNSNNFERVQIGNIIQNDLEKLGMKVNLLPVEFNTLVNKLTISRDWEGVIIGLTG
                     GIEPHGGKNVWNSKGQLHFWNFGNKRNYEWEKEIDHLFEIGTKYLNQEERKKIYDRWQ
                     EIVTDYLPLIYTANSNVIYAVRNKFENMKITVYGGVLHNIEEIKIKENGKF"
     gene            31075..31602
                     /locus_tag="PLF90_00785"
     CDS             31075..31602
                     /locus_tag="PLF90_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOK55852.1"
                     /translation="MESFRLRVFYKKEGISRFISHLSFCKLIERSLRRLELPLKFSEG
                     FTPHLKISFCPPLPIPISGNNEFFEIEVYEKFNLESFVKDINLILPEGTVVKRCYWIS
                     HKFSISSIYGTYSIPLKEPIIKEKAEEFGKIIEEKEEFIKVIFKMENFSHKKIFVNGI
                     FDGITRELFIENEQI"
     gene            31589..33025
                     /locus_tag="PLF90_00790"
     CDS             31589..33025
                     /locus_tag="PLF90_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOK55853.1"
                     /translation="MNKYRIFISNEKVILRVLIEKEGKIDNIFIHKYDFEPDIGNIYK
                     GRVEKFLYGLNSAFVNIGEMKSGFLQLNKSEFYFSLEENEDISGHQILKEGKEVLVQI
                     CKPGEDEKSPKVTEKVVLPGRFFVLIPNLRIQKISKKIEDIREKTRLMKIFKKTLGKK
                     FGFIIRTAALNKKEFYIYREIEHILNIWKRIKRDYKRKKTPSLLWKELPLYLKVLRDY
                     TDENCEFVEIDDENIYREVVKYVNLFIPELKGKIYFYRNKIPMFVKYGFEEKFESFIS
                     KTVLLPSGGYLIIEKGKTLTAIDVNSGKMEKEDFEETIFKTNMEAAEEIPRQIRCRNL
                     SGLIIVDFIDMKGKFKEKVFKKFIENIKGDKSRINILSISKLGLVEMAREKNNYSIYN
                     LLLKECPFCDGSGIEKGKEIVYIQLRKKIFEIFSNKAYNGKLEIEVSQGLFEFIFKNQ
                     LFKNFLYSDRIKFKVNGKLKEDEFKIFI"
     gene            33044..33811
                     /locus_tag="PLF90_00795"
     CDS             33044..33811
                     /locus_tag="PLF90_00795"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HOK55854.1"
                     /translation="MAMHVKIEEKEGYQKILTPENSDLKLLSFSILRQKEGIFEGNTE
                     KEETVFVITEGKCNFYIEKNLIGTMKRKNVFEEPPVAVYLPPYYKYKIEFEKKTEICI
                     VGCQGKGTGKPKFLNSKEIKFRRVGEETYFRNITDIITDAFPSEKLLLGETINDPGNW
                     SSYPPHKHDKNNPPEEYCLEELYFFKIHPQKGFGVIRIFDENEDNLFLIQNNEVVTIP
                     KGYHPVGVIPKHQIYYLWALAGEKRILKPKVHPDFTL"
     gene            33825..34367
                     /locus_tag="PLF90_00800"
     CDS             33825..34367
                     /locus_tag="PLF90_00800"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOK55855.1"
                     /translation="MVPKKVFLTKGVGKDKERLTSFEKALRDAGIAQFNLVSISSIFP
                     PGCKLIPKKEGLKLLKPGQIVYVVMSRNSTNEPHRLITASVGMTIPKDPNQYGYLSEH
                     EGFGETEEKAGDYAEDLAATMLATILGLEFDPMSSYDEKKEIWKMSGRIVKTTNITQS
                     AIGDKNGLWTTVVAAAVFIL"
     gene            34389..35615
                     /locus_tag="PLF90_00805"
     CDS             34389..35615
                     /locus_tag="PLF90_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55856.1"
                     /translation="MEKDYQIIRKLAEKVKEIANHPVQKEKAEIWKKHNKLERVRPLV
                     LIFPEGSWREILPENEILCEDTFLRRIEWDLRQRIYYWEKIKDDNVIDGKVYSPIVIK
                     NTGFGVEVKDIRPEFAYGARHFEPVIKEEKDIEKLKKPEISIDWEETERLYKKTCEAV
                     GGILEVEKRGPSHPWFAPIDWFITVRGIDNFFMDMVDRPEWLHRILNFITECKIEEWE
                     FYEKNNVLSLNNGPHYVGSGGVGFTDQLPQPDFDGIHVRIKDLWGHATTQIFSLVSPQ
                     MHEEFALKYEGRFLSKFGFNCYGCCEPLHKKIDIIFKHIPRLRRLSMSPWVDIGEAAE
                     KLGKRAIFSWKPNPSFMASDTWEDDYIRKTITDACEKTKNCVIEIILKDIHTCRNKPE
                     RISNWVKIAKEIAEKY"
     gene            35616..36413
                     /locus_tag="PLF90_00810"
     CDS             35616..36413
                     /locus_tag="PLF90_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006036176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOK55857.1"
                     /translation="MENKKKISIWEDKIPYSKGNSEEDIPKLTPYLIENKKNNPCIIV
                     FPGGGYSVRAPHEKEPIALWLNRIGISAFVLDYRVSPYRYPVPFLDAQRSIRFVRYNA
                     EKFNIDPNRIGIIGFSAGGHLASLTGVHFDYGKKENDPIEKISLRPDLLILCYPVISF
                     LNYSHQGCIKNFLGENIDKEILEFLSSEKQVKKDTPPCFIWHTQNDDVVPVQHSILFA
                     LSLKEKNIDFELHIFEKGLHGLGLAENDFSVSYWTILCEKWLRKHNF"
     gene            36426..37715
                     /locus_tag="PLF90_00815"
     CDS             36426..37715
                     /locus_tag="PLF90_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOK55858.1"
                     /translation="MKIAIIGAGSLGFTKRFIIDIISVPELRNFTLALNDIDEERLNL
                     ITKWANALKKQENLKIEILSSTDRKDVIKNSDYIINTIRVGGLEAFELDVSIPRKYGV
                     HQAVGDTVGPGGVFYALRTIPVLLEICEDIKKLCPDAWFLNYTNPMAMNTWAMIKYGH
                     KKVVGLCHSIQGTAWQLSSYIGVPYEEVYYWAAGINHMSWFLEFKRNGKDLYPELFEA
                     MKNPEIFKKDPVRFEIMKHFGYFVSESSFHMAEYVPYFNKKKELINKFNTHGWIYLDV
                     CKNAYDPATEKLKKEAEGKEKIEISRSNEYCSYIIHALETGCILRFNGNVLNDEIIDN
                     LPYGCCVEVPCYAEKGNIRPAKVGNLPIQCASLNRLNINVQEITVEAAIKRKKSLVYQ
                     AVALDPLTGAVLSLEEIKNMVDEMFEKEKEYLPEFTD"
     gene            37722..38189
                     /gene="ispF"
                     /locus_tag="PLF90_00820"
     CDS             37722..38189
                     /gene="ispF"
                     /locus_tag="PLF90_00820"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOK55859.1"
                     /translation="MYKTGIGFDIHKFAEGRKFIIGGIEIPYEKGLIGHSDGDVLIHS
                     ISDAILGALGRPDIGYYFPDTDEKIKGIDSKEILKRVIGFLKDDRYEIVNIDSVIICE
                     KPKILPYSEKIKKSLSEVIGIEKEKINIKGKTTEGIDKGNYCACISIVLIERF"
BASE COUNT        13479 a         5093 c         5675 g        13997 t
ORIGIN      
        1 tttaatagtt gataatttta aactcgttga ggcagttttt aatagagtag gagagttaat
       61 ttatgaattt tataagaatg tgattgaaat agataatgtt gaagggtttt ttcagggaga
      121 tgaccttgga tttaaaacat ccacattttt atcacctgaa atgttaaaaa aacttgtaat
      181 acaatggcat aaaaaatttg cagaaattgc tcacatgaga ggaaaaatgt actggtttca
      241 ttgctgtggc aatattttaa atgttattga tgattttata catgatgtta aaatagatgc
      301 ctttcattct tttcaggatg agattatacc tgtatgggaa ttcaaggaaa aatatggaaa
      361 taaaattgct gttcttggag gagttgatgt aaataaactt gcaacctatg atgagaaaaa
      421 tttaagaaaa tatgtgaggg aaattcttga tagatgtatg ccttacagat atgctcttgg
      481 aagtgggaat tcaatcacaa attatatacc acctgaaaat tacttgatta tgcttgatga
      541 aggcttaaga taaattttac tgaaccatat ttactttttt attttgttat attctgtttt
      601 ttcctgtcaa ctacgtcttt cttaaagggg tttaaacccc tttaaaatcc ccaagaggta
      661 tcaactcccc taaataggtg gctcaccatc tcgccacccg tgctaatcgg tgctccttat
      721 tcgtcgcacc agcaccccct caatctttga gggggactta aggtgttttt atctattctg
      781 ggcaaaaaac gccagaaaaa acacttggaa tttttcaaat tccagtgttt tatacctgta
      841 gttgagcaag ttttacggtt taaggtactt ttaaatttta aaactatatc cttcttcaag
      901 cataattata aaattttctg gtggtatata atttgctata gaatttccag aaccgagtat
      961 ataacctgaa cccgcacatt tctcaaggat ttttctaata tattttctta aatcattttc
     1021 cttcaatgta cacaactttc cgatatcaac tcctcctatt attccaattt tctctccata
     1081 tttctccttg aacttccaga caggtaatat ttcatcttca aaagaatgtt tagcatcaat
     1141 ctcaacctca tttattaagt catccataat tggttcaata tttccgcagg agtgtaagac
     1201 ataaattttt ccattttgat gggcaagttc tgaaagtttt ttatgccatg gaagaatata
     1261 ttttttgaaa aattctggtg gtaaaagcat ttgtgtttta aatcccatat catctccttg
     1321 aaaaatccct ataatttttt ctttttcaat aaacttttta gttgctttat atattatttc
     1381 tccaattttt tcagaagttt tctttacaat ttcttcatta tcataaatta aataacatag
     1441 attttcaaaa ccaaataaaa acattaaggt ctcaaaaatt ccctgagaaa tacatagaaa
     1501 aatacccata ttttcaggta aaatttcgga gagatattca taaacccaga tatctacctt
     1561 ttcaggttca ggccaggtat aattttcaaa atcattccag ttttttataa caccttcttt
     1621 ttcctcagcc cattttcttt tgccttttga gaacagagaa gtatcatctg tttctctttt
     1681 cggtatttca aattttaggt ttgaagtaaa tctgaaatca gaacttaatc ttattgtgtc
     1741 aaatccaagt ttgtaatata aatttacaac attttgaatt gcttttttct gtgtttcata
     1801 atcaatgggt tcaatccatt ttattccaaa ataatcttcc aataaaaatt ttagaatttc
     1861 tttatcaata tgtagttccg cacaatgtat tctacttccc ttaacttctc ttaaaagaac
     1921 ttttttaaaa tcatttaaat ctggttcagg aaattttatt ttcatttgaa actattatac
     1981 aggattaaaa taataaggta aaattaaaaa aatggaaaag gaaataaaaa gatatagaat
     2041 aagaccaaaa acttacctgt gtttaactcc tgaagaagca attacaactg gaataagtac
     2101 aggagaacaa ataattgagg aattaatact tgttagagaa tttacacttt ctgcttatat
     2161 gtatggagtt acaagaaacc tgccagaaga agaattaaaa aaaatgaaag tagaagagag
     2221 gggagagaaa ttatttgaaa gcaggaaaga agctataaat tatctaaaag aaaaaggaga
     2281 aaaatttttt tctctttata tagttaaaga aacaagaatg tttccgagag aagaaataaa
     2341 atgggatgaa acagaaaaat caattgagga ttttctgtcc cagttaaaag aatttgaaaa
     2401 ataaaactta ataaattctg aggtttctaa aaaaattttt gaaagttttt tgtaaattta
     2461 aaatattttc ttaaaacagg agaattaaat gattgagaaa tattttaaat tcagagaaag
     2521 gggcacggat ttaaaaacag aaattatcgc aggaattaca acttttataa caatggcata
     2581 tataattttt gtccagccag cggttttatc acaaggagga atggattttg gagcagttat
     2641 ggtagcaaca tgtttttcat ccgcaattgc gactttaatt atgggtttat atgcaaatta
     2701 tccaattgct cttgctcctg gtatgggaga aaatttttat tttgtttata ctgttattct
     2761 tggaatgggc attgcatggc aaaaggttct tggagcagtt tttatttctg gtattctttt
     2821 tataattctt tctctctgga aagtaagaga aaaacttgtt gagaatgtcc cttcttcttt
     2881 acaatgtgga atagcaggtg gaattggttt atttattgca ttaattgggt ttatacagag
     2941 tggaattgtc cagaagggcg gagttgtttt aacaatgggt aatatttatc aaaaagaagt
     3001 aattttgtca ataatcggac ttataataat ggggactctt atggctttaa aggtaaaggg
     3061 aaacatttta attggaatga ttataactgc aataatagga atttgttcag gaataataaa
     3121 gtatgagggg ataattggga aaattccttc tattgctcca acattcatga aacttgatgt
     3181 tattggtgct ttaagagaag ggatttttac agtaattttc gtttttcttt ttatggattt
     3241 atttgatact gttggaacag caataggagt tggagaggcg ggtggattta taaaaaatgg
     3301 gaaattccca aaggttgaga gagttttatt atcagatgca gtaggaactg ttgttggtgc
     3361 aatttttggg acaagtacag tgacaagtta tattgaaagt gcaagtggaa tatctgttgg
     3421 agggaggact ggttttgctt ctgtaatcac tgcagttttg tttttactct ctttattttt
     3481 ctatcctttt gtaaagatga ttgggacagg aattcaaaca gttggtggta taaccttata
     3541 tcctgtaaca gcacctgctt taattattgt tggaagttta ataataccaa ctataagaaa
     3601 aataaaatgg gatgacccaa cagaatcaat tcctgccttt ctttcctttg ttggaattcc
     3661 tttcacatat aacataggag aaggaattgc ttttggattt atatcctatt tccttttaaa
     3721 acttttcaca ggtaaataca gaaatttaaa tcctgttgtt attattgttg cagttgcttt
     3781 cattttaaga tttattcttt taaaaaatta aaattatttt acttcccatt ttttaattaa
     3841 tgaaactttt ctaagtatat tttcgtctaa ataaaaaagg gagataaaaa atgacttgct
     3901 ggttttttga tagaggtggg aattctctga aagatgcagt aataatatat gctgacagtt
     3961 ttgttgaaag gataggtatt atatttgatt atttgagttt aaaacatgga aagataggtt
     4021 ctgactggag agttttcagt attcaacgaa ttcgtgataa agatagaaat tacgaaatct
     4081 ggagtataga aatttataaa gaaagaaaga taaaggagat taagacatat tattttgaaa
     4141 tttcaggagt taaaaattca aaaaaaactg aaactttttt taagttcctt tcctgaataa
     4201 aaattgaatc tttctacata aggtcttctt caactcttat aaaaacagtg ggatttttta
     4261 ttacatccag tttatctatt tcctttattg ctttttttac atttttttcc tttgctttat
     4321 gagttagcat tactatagga accgcttttt ctggattgtc ctgtttttga attacagata
     4381 aaatacttat atcattatct ccaagaattt ttgctatttt tgcaagaact ccaggtttat
     4441 caattgctgt aaatctgaaa taatattttg tttcaatttc ttcaaatggt tttaaagaga
     4501 tgatatcttc tgacacaaaa ttttctttaa caaaaccttc agaaacaatc tttttactta
     4561 tatccacaac gtcacctatt acagaactag ttgctgcatt tcctcctgct ccttctccat
     4621 aaaaaagtga ttttccaatt aaatcacctt caacataaac agcattatta acaccttcaa
     4681 ctagagataa aagatgagtt tttggaagca gagcagggtg aactcttatt tctatttgat
     4741 ttttttcttt tctacatact ccaagcagtt taattctata cccaaaatca ctcgcaaatt
     4801 ttatatcaat tgtttgtatt ttatctattc cttcaacata aatttcttca gcagaaaaaa
     4861 gttctccata ggtgatgtat gtaagtatta aaattttgtg aagtgtgtca taacctttta
     4921 tatcaagagt tgggtctgct tccgcatatc caagtttttt tgcttcctca atcccttttt
     4981 caaaatcaat tcccttatga tacatttctg aaaggatata atttgttgtt ccattaagta
     5041 taccaagaat ttttgttatt ttatttcctg taaaactttc ttttaatgct tttataattg
     5101 gtattgctcc tgcaacactt ccttcaaaac caatatatac atttttttct ttcgcttttt
     5161 taaataattc ctttccatat tttgataata atgctttatt tgctgtaaca atagattttc
     5221 catttttaat tgcctttgta acaaattcgt aagcataatc tgttcctccg attaattcaa
     5281 cgactatatc aatattttta ttttcaatta tttcatcagg ggattcagca gagatatctt
     5341 taaattgatt gagttttcct ttatccttat caaaaacttt taaaatattt atctcaattc
     5401 ctgttttttc ttttataata ttttttcttg aaattaattt ttttataaaa cttgaaccaa
     5461 cagttccaca accaataact ccaatattaa tagatttttt tttcattttt cagtaaagag
     5521 aatttggaat tgtatttttg tatagtgatt tagaagcaaa aaataattca ttctgtatac
     5581 tctgaacttt attttctaat tcttcttttc taatatttct tgtttctctc aacttttttg
     5641 cttctgcata aaattttcta cttaaaaaat aattatagga tggttctatc ccttttttta
     5701 attcatcaag tgccttatct gcaagaataa gtttaacctt atattcattt tcataggttt
     5761 tattaagaat tttatttcta agatttaaca cttcttttac tggtggtatt tttttaactt
     5821 caattctaac ataaaaatat ttgtaaatgt cttcattttc tctttcaagt tttttgagtt
     5881 ttgaaagata tgtattaatc tctattttat gaaaaatctc atttattcta tccgttgaaa
     5941 aattaaattg ctttgccagg gcaagtgctt tttcagaaat ttcttttacc ttaatataat
     6001 cacctgcttt aaagtatttt tccatttctg ttaaaagatt ttcaaattct ttttgttttt
     6061 cttcaagtag ttttctctgt ttttcttttt caagttcttc ctgtcttctt ttttcttctt
     6121 ctaccagttt tcttaatttt tcagttttgt atttattata atagaaccaa ccaccgccaa
     6181 gaattccaaa tatcaagagt accattacaa tctttttgat ttttttcaat agttttttca
     6241 taatttacct ccaataataa ttttaccttt cctgatataa catttcaaat aaaattttgg
     6301 cttatgttaa ttcttaaatt aaaatacatt ttttaattct tttttaaact gataaatcaa
     6361 ttcttccact tcatctattt ttccatctat tattttttgt aaaaagaaac tacctataat
     6421 tattccatct acatattcct taatctgttt tatctgttct ttagtggaaa tcccgaaacc
     6481 aagagcaact ggaatttctt ttaaagattt tacttttttt atcacatcaa ttatttccgg
     6541 atttaaacta tctcgttgtc ctgtaactcc tgcaacactt ataaaatata tgaaaccatt
     6601 tgccattctg gttatttttt tcattctttt ttcagaagta aatggcgtta aaaaataaat
     6661 tagattcatc tctctttttt tcaaaatttc tttaatttca aaactttctt catatggcag
     6721 gtcaggtata attaaaccag aaaccccaca ttcaacacat ctatcagcaa atttttcaat
     6781 tccatatctg taaacaggat taaaataagt cataaaaaga tagggtatat tcatttcatt
     6841 tttaagtatt ttagcaagtt caaaacattt attagtattc attccatttt caagtgcctt
     6901 tgctgatgta tattgaatta caggtccatc tgcgattggg tcagaaaaag gtattcctat
     6961 ttcaattata tcagcacctt tttcagcact ttttttaatg atttcaattg aattctcaaa
     7021 agaaggaaat ccagcagtaa aatataaaat cagtgctttc tcttttttta tcttcaattc
     7081 tttaaatttt ttttcaatct gtgtcataaa tcaccttcta tttttgctac ttccataaca
     7141 tctttatcgc ctctacccga aagacagaca accactatat ctttttcttt aaatctgttt
     7201 ttattttcga tcagccagcc aattgcatgt gaagattcta aagcagggat tatcccttca
     7261 agttcactta aaatatgaaa accatttaaa gcagttttat catcaacagc atagtattca
     7321 gcccttcctg tttctttata aaatgcatgt tctggtccaa ctccaggata atcaagtcct
     7381 gctgagattg aatgagttgt ctttatctgt ccgtaattgt cctgcaaaac ataggaaaaa
     7441 ctaccatgta atattccttt ttctcctttt aatagagttg ctgaatgttt atctgtattt
     7501 attccttttc ctcccgcttc aactcctata aattttactt ctttatttct gtaaaagggg
     7561 aaaaacaaac ccaaactatt gcttcctcca ccaacacagg cgacaagata atcgggtaat
     7621 tttttttctt tttttaatat ctgttttttt gtttctttac ctataactga ctgaaaatcc
     7681 cttacaatca taggataagg atgtggacca actacagaac caattacata atgagttgtt
     7741 ttaaaatttg ttatccagtc cctcatcgct tcatttattg catctttcaa ggttttactt
     7801 cctgaactaa caggaacaac ttttgttccg agtaattcca ttctgaaaac atttaatttc
     7861 tgtctttcaa catctatttc acccatataa acaacgcatc ccatatttaa caaagcagaa
     7921 gcagtagcag ttgcaactcc atgctgacct gctcctgttt ctgctattat ccttttcttt
     7981 cccatcattt ttgcaaggag aacctgaccg agagtattgt ttattttatg agcacctgta
     8041 aatgctaaat cctctctttt taaataaact ttaaaaccaa gatatttact gaaattttct
     8101 gcataataaa gaggggttgg tcttcctgca tattctttta ataagtaatt cagtttttct
     8161 ttaaattctt ttgtctttga tattttttta tatgcagttt caagttcttc aagaggtccc
     8221 attaatgttt ccggagcaaa tcttccacca aaaattccaa attttccttt tctgtcaggt
     8281 aacattttta acctcttcta ttaatttttt tattttttca atatctttta tccctggagc
     8341 aatttcaact cctgaggcaa catctattcc aaaaggtcta tattttttaa ttatattttt
     8401 aacattttca ggtgttattc caccggcaat aaatattttg ttccatggta atttcccttt
     8461 ttcaattttt tctttatcta tcatttttcc agttccgcca aaactatctt cttcgtatct
     8521 gtctaataag aagtaatcgg ccatcttata atgcttcatc ctttttacaa tatcttcata
     8581 atttttaact cttattgctt ttataatgat tttatcagga aaattttttt ttaatttttc
     8641 taaaagcgaa ggcggttcat tcccatgaag ttgaattcca tctattttaa gttttttcac
     8701 agtttcaaat aaattttttt cgtcaggatt tacaaaaagc cctattttat ggatattact
     8761 cggtaaattt tcaattaaca attttgcttc cctttcttca acaaatcttg gacttttttc
     8821 ataaagattt atcccaatcc attcaattcc aagatttact gcatcaatta tatcttttct
     8881 tcttttaaaa ccacagattt ttatttcaat cattaaattt caggtaaatc cttcaatgct
     8941 tcctttattt tttcttctgg atattcataa tcttcaagtt taccttcaaa atatgcttca
     9001 taggcagata aatcaaaatg cccgtgccct gaatgattat atacaattac ctttccttca
     9061 ggagcatcct ttgcttcatc aataactgct cttatagcat gtgttgtttc tggtgctggt
     9121 aaaaatcctt ctgtttgagc aaatagaact ccggcttcga aacattctct ttgatgatat
     9181 gctactgctt caacaagtcc aagtttatat aagtgacaca gtaaaggagc atctccgtga
     9241 tatcttaaac cgcctgcgtg aattcctggt ggaacaaaac tatgtccaag tgtatacatt
     9301 ttgagtaaag gagttgtaca ggcagtatca ccaaaatcat atctgtaaag tccttttgtt
     9361 aatgtaggac atgcttttgg ttctactgct ataaatctta tgttttctcc ttttatttta
     9421 tcaggaacaa agggaattat aaatcctcca aaattacttc caccaccaac acatccaatt
     9481 agaacatctg gtttttctcc aataagttgg agttgttttt ttgtttcaag ccctataatg
     9541 gtttggtgaa gtaaaacatg atttagaaca cttccgagag aatattttgt gtcttctctt
     9601 ttaaccgctt cttcaattgc ttctgatatc gctattccta aactccccgg tgaattcgga
     9661 ttctgcgaaa gaattttcct tccaaattca gttttatctg atggagaagc atacatagtt
     9721 gctccccatg attgcgcaag taatcttctg tatggttttt gataatagga tacctttacc
     9781 atgaaaatta cacattcaag gtcgaaaagt ttacatgcaa aagcaagagc acttccccat
     9841 tggcctgcac ctgtttcggt tgttaatctt tttacccctt caattttgtt gtaatatgcc
     9901 tgagctattg ctgtattcgg tttatgagaa ccaggtggag atacagattc attcttaaaa
     9961 taaattcttg cctttgtttt caatgctttt tcaaaatttc ttgccctgtg gagaggagtt
    10021 ggcctataaa ttttatatgc attcaaaact tcatttggaa tttcaatcca tctttcaggt
    10081 gcaaattcct gcataattaa agacattggg aaaataggcg caaggtcttc cggttttaca
    10141 ggttcttttg tttttggatg aattacaggt ggaagtggtt ccggtaaatc aggaagaata
    10201 ttataccatg cctgtggcaa ttccctttca gacagataaa ttttattttc ttccattttc
    10261 ttttcctcct ttcttttaat tttttaattc ttttattttc tcttctacat tcttactttt
    10321 taaaatactt tctccaatca atacaccttt caaacccaaa ttttttaaaa gcaagacctc
    10381 ttctttctct tttataccac tttcacttat gacaattatt ttatcaactg gaatatgttt
    10441 aatcaagttt aatgtattgt ttatatcaac tttaagtgtt ttcaaatctc tattgtttat
    10501 accaagtatt ttgtctttcc atctcttgat taaattagaa actcttttcc aatcattgat
    10561 atcaaatatt tctattaaaa tttcaatgtt tgtttcctca cagattttta taaatttttc
    10621 aagttttcta tcttcaagta ttctaactat caaaaggata aaatcagcac cataatattt
    10681 cccttcataa atctggtatt catctacaat aaaatctttc atcagaacag gaatttcaac
    10741 tgattctttt acagttttta ggtcatctat agaaccaaga aaatatggtt cacaggttaa
    10801 aacagaaata ccactaactc cggatttttg gtatttgata gcagttgatt tgaaatctaa
    10861 attattatta attgtacctg cagaaggaga tgctcttttt aattcagcaa taattccaaa
    10921 ctcattcttt aaagattgat aaatgctgta ttttttcttt aaagaaatca ttcctatcaa
    10981 tttttcagta ggtaaaattt ttttcttttc ttcaattatt attgattttt tttcaataat
    11041 ttcagaaatt aaatcactca tttttttcct tttctatctg ttttataaat ttttctgcaa
    11101 tttcttttct ttcaggatat ttttctttta ttattttcca ttcttcaatt gccttcttta
    11161 aatttttttg tttcatataa atatgtgctt taagattttc tatataaaaa ggatggttat
    11221 aattctcggc cttttcaaga tatttaagag aattttcaaa atcattttca ttataataaa
    11281 accatgcaat ttcccagtat aatctataat cgtcgggttt tttttctatc ccttttttca
    11341 taaattcaat tgcaaatttt tttatttctt ctttcttttc accttcatat tttggagcaa
    11401 taccatttat caagaaccag ccaccaagac agtaggcatt tacatcatcg gggaaaattc
    11461 tggtaattaa ataaaaaaca ggaaaaattt ttgaatattc acctttatgc cagtaattat
    11521 caactgtaag ataaagtata tcaacaattc catctgtcaa atctggaaaa ttacacaaat
    11581 caactgttgc ctgttttttt aattcaattt caatgttttc acccgaaatt gcaaaaccaa
    11641 aacagaataa aaaaagaatt aaaatttttt tcattttttt atatattcaa ggatttcatt
    11701 gaattttcta taaggtattc ctttttttaa aatttcatct gttaattcaa ttcctttttc
    11761 aattctatca acaatacccg ttgttttaag gagaaatgcg gtatttaagc agacaatatc
    11821 ttttttaggt cctttttctt tacctctaat tatattaaat aaaatctcag cattttcttt
    11881 taaatttcct ccttttatat cttcaatttt acatcttttt aaattaaaat cctctggaaa
    11941 tatttcaaat tcacttattt cttcatcaat ttcaactata tatgttttcc ctgtaagtgt
    12001 tatttcatca agaccatctt cagagtgaaa tacatatccc tttattccaa gatttttaaa
    12061 aacataagga ataattttta aaagagaata atcattcaca cccataatct gataatttgt
    12121 taaagcagga ttacacaaag gtccaattat attaaaaatt gttttaaagc caagttcttt
    12181 tctaacttct gctacatttt tcattgcttt atgatataag ggagcaaata aaaaagcaaa
    12241 tccaaacttt tcaagtaaaa tttcgctttt ttcaggctca atatctattt tatacccaag
    12301 atattccagt acatcagcac ttccacagga acttgtgata gacctgtttc catgttttgc
    12361 aactgttagt cccatagaag aagcaacaat tgcacatact gttgagatat taaatgtacc
    12421 tgaataatca ccacctgttc cacaggtatc acaaattatt tcttttttgt gtcttatttt
    12481 cagcatttta tctcttaaaa gttttgctgc accggttatc tcttctgaac tttctttttt
    12541 aatttttaat aatgttagaa atgcagcagt ttttacagaa gataattctc cttccataat
    12601 tttgttaaaa atttcatatg tttcatttgt agtaagattt atattttcaa aaagtttatt
    12661 gaatattttt tccattttac cctccctgta aaaaataaat atagagaaaa ataggaaaaa
    12721 aacaggaatg ggataaaaaa atttaacgcc aagagaggaa atttcttaaa taattctttt
    12781 tcagaacaat cctaaattca tttttatttt ttcccataga aatttaattt aacataattt
    12841 ttgaatttgt caaattttta ttctataatt ttaaaaaagt tagtttaaac aaactttttt
    12901 agttaatact aacaaaataa aaaatgaaaa aattggtaga ggcaaaagaa ggaaaataca
    12961 aagtggttga tgtgctgaaa catgggaaat taaaaaaatt atgtaatatg gggatttttg
    13021 ttggagatat tattgaagtg gttaaacctg ctcccggtcc tgtaataata aaaaaaggaa
    13081 atataaccat cgggattgga tgtggaatag caatggacat aatagttgaa aaagttgaat
    13141 gaaaatggaa gagaaaatag ttgcaattgc agggaatcct aatacaggaa aaacgacgat
    13201 attcaattct cttactggaa caagacaaca ggttggtaac tggccaggtg taacagttga
    13261 aaaaaaggaa ggatattata tttataaaga tgtaaaattt aaagttgttg atttaccggg
    13321 aacttatacc ttaacaggag aaagcgaaga ccagaaaata gcaattgatt ttattactca
    13381 aaatgaagta gatgttttaa ttataatggc agatgctttg aatcttgaaa gaagtttata
    13441 tcttttactt ttactctcag agtttcacaa aaatattatt attgacctta atatggtgga
    13501 tttggcagag aaagaaggaa ttgaatatga aattcaacat ttagaaaaat tgcttaaagt
    13561 aaaatttgta aaaacagttg gaaataaaaa tattggtata gaagatttga aagaagaaat
    13621 ttataaaaag tgtaaagaac caccttctga attttttgaa tttaattatg gtgaagaaat
    13681 tgaaaaagtt atctctgaaa ttgtctgtca atttaaaaac ttaaaacttc cttatcctcc
    13741 aagatttctt gctataaaaa ttttggaaaa agatgagtat tttacaaatt ttataattac
    13801 aaaagttgat tggaaaaaaa ttaacgattt gataatgaat acagaaaaat ttttaaataa
    13861 accattagat agagttttaa tggaaaaaag atatggaata attcatggaa ttgtaaaaga
    13921 gtcacttatt gagaaaaaga aagaattgaa accggaaatt acagaaaaag ttgatagaat
    13981 tttaactcat aattttttag gtcctttatt ttttcttttt ataatcttta tcatattcaa
    14041 tgctgttttt gtctggggaa aaccatttgt taattttatt gaacttatac ttgaaaaatt
    14101 aaagattgtt ttttcatatc taaatctacc tatttggatt aattctttga taaaagatgg
    14161 aataatttct ggtgtcggtt ctgtaatatt atttcttcca aacatttttc tcttcttttt
    14221 tttctttgca atccttgaag ataccggcta tatggcaaga gcggcaattg ttgcagatag
    14281 aataatgcat aaggtaggac ttcatgggaa gagtgctatt cctttaattt taggttttgg
    14341 atgtaatgtt cctgcgataa tggggacaag aattcttgaa aataaaaggg ataaaatctt
    14401 aacttcccta tcaatacctt ttatgagttg ttcggcacgg cttcctgttt atcttttatt
    14461 tacaggcatt ttttttaagg aaaatcaggg aatgatagtt ttttcattgt atttgttagg
    14521 aattggtatt ggtgttttaa gtataaaaat ttttggttcc atttttttaa aagatgaatc
    14581 tctaccttta ataatagaat taccaccttt cagaatgcct tactggaaaa atgtattgat
    14641 tgagagttgg acaagaacaa aatacttttt gaagaaagca gggaccatta tatttcttgg
    14701 aactttaatt ttatgggttt taaactattt cccgcatcct tataattatg gaaccgaaat
    14761 gaattttata ggtaaattgg gtagttttat ttttccatta tttaaatttt ctggttttgg
    14821 tttctggcag gcaggggttg cacttatttt tggaatatat gcaaaagaac ttgttgttgt
    14881 aacttttggg actttatttg gagttgagaa tttaacagaa acattaagat tctattttac
    14941 ccctatctct gcgtattctt ttatgcttat gagtttactg tacatgccat gtcttgctac
    15001 atttttaatt ttgaagaaag aagtaggtat aaaatgggct ttaatttctc ttttttggtc
    15061 tttatttgct ggatggtttt tttcaacctt attttatcaa attttttcat ttttaaaagg
    15121 aggaacattt gttaactaaa agcttagagg attatcttga agcaataaaa atactcacga
    15181 tagacaaaaa agtcacaagg gtaaaagaaa taagtgaact tcttgatgta aaaaaatcgt
    15241 ctgttgtgaa tgcattgaat attctaaaag ataaaggata cataaaacag gaaaaatacg
    15301 gttatattga attgacagaa agtggtgata gagaagcaac gaagatatta aataagcata
    15361 agatagtaat gaagttttta acaaaaattt taaatgtttc tgatagtata gcagaaaatg
    15421 atgcatgtaa aattgaacat tttatcagtg aagaaacatt gaaaaaaatg attgtattac
    15481 tggggaaaaa aggtaatcat ttaaatttgt ttaagaaaca aggtaggata aacaaagatt
    15541 gatgtaattc cggagtatag aaaaataaat tttcttttat ttttttgttt ggtttttttt
    15601 caatctttat attaccttta attgctataa taaatatcca atttggaaaa agaccaaaaa
    15661 caggtgagga ataaattttt acatttttaa atactttttt taagtcagaa taaactgttt
    15721 ttattctctc cggatgaaga aaaatagatg ttccaccttg agttacgtaa ataccatttt
    15781 tgtttagatg tttataacag agtttaaaaa attcaacagt atataatgga caggatggta
    15841 tttctccagg gtcggtagca tcaactataa taacatcata ttttttatta ttctctttta
    15901 tatatttaaa accatcttct attatgattt ctgctctttt atcttcaaaa gcacctttat
    15961 caaattttaa atattttttt gcaattttaa tcatttcttc atctatatca accattttta
    16021 cttctttaac tggatgcttc aaaacttctc ttaaagttgc accttctcca ccaccaatta
    16081 ttaaaacatt ttttggttca ggatgggaaa acattgcagg atgaacaagt atttcatgat
    16141 aactttcttc tcccttttca actgattgaa ttaaattatc tataattaaa actttaccaa
    16201 aattctttgt ttcatatacc tcaattttct gatactttgt tcttttttct aataactttt
    16261 ttttgatttc aaaagcaatt cctacaccat aaggatgata ccagtcaata attatatttc
    16321 tatttttttg catatattcc tctttcaatc tttataatag atggtttttt gctttttaat
    16381 tctttaataa ataaattact cgctttttca ggaaatgttt tttctccaca ggtgtatata
    16441 tcaactccag catatttttt ttcaggccat gtatgaatac ttatatggct ttctgaaatt
    16501 aaagcaaacc cgctgactcc cacaggtgaa aatttatgaa ttttaacatc aattaaagtt
    16561 gctttagatt tttctacagc ttttaataag atttttttta ttttttcagg ggagttaaga
    16621 atattaaaat cggaacattc ataaaggtct aaaattatat gaacaccgtt tggaggaaca
    16681 aatgtttgga atttcctcat ttttccctcc tttttgtctc atttttcacc tcccttccca
    16741 attaatttaa aaactttttc ttgttaatat agatataata ataaaaaatt tttcaaaaaa
    16801 tgtcaattca cttctaaaaa tattttttaa caaaaaggtt tacgattttt ctaaaaaatg
    16861 gtattttatt cctgaaaaga agagagaaga aatatgaatg aacttgaatt tataaaaaaa
    16921 gttgtaattt ctggtatttt aagtggaatt attggttttg aaagagaaaa acatggaagg
    16981 gaagcgggtt taagaacgat gattcttgtt ggaatgggta caactacgat tttaatcaca
    17041 tcttttcaag tttattatat tttttcaaat tttcaaaatt ctgttttgag aattgaccct
    17101 gcaaggattg cttatggagt ggtaacagga atgggatttc ttggagcagg agcaattata
    17161 aaggacagta aaagaataag gggtttaact actgcagcat gtttatggtt tgtaacatct
    17221 gttggtcttg cagtaggggt tgacctgtat attctggctt gttttttaac ttttatttcg
    17281 ttaataatac tttacttttt aaaaattttt gaaaaaaaag ttccagttga tacatatagt
    17341 actcttacaa taattggaga agataagaga gaattaaaag ataaaataga aaaaatattg
    17401 aaaaattacg gttttaaaat actttcaaca gaaatagaaa aaaataaagt taaagcaaaa
    17461 atcaaattga catatacaat cagatataaa tcaaaccaaa atgtaaatga actttttgaa
    17521 aaaatatctg aaattgagga gttaaagaat ataaaattaa actaaaatgt gaaaaagttt
    17581 ttaactccaa gagtaacaaa ggaaacaata atccccgcca ttaaaactcc tccgataata
    17641 cctattaaaa aatttttgaa ttttaattga aaaattacag atgcaattgt tcctgtccat
    17701 gcaccagtca ttggcagagg aattccaaca aaaattaaaa gtccaattgt tttataaatt
    17761 tcaataattt cggatttact ttttgtccta tttattaacc atgtattaaa tttttttccg
    17821 taattgaatt tttcaaggaa ttttaatatt ctttctagaa aaaaatatag tggaattaca
    17881 ggtaaaagat ttccaagaat tgaaattaaa aatacattga aaaaaggaag tgacttactt
    17941 aaaccataag gtatcgcgcc tcttaattct gaaatcggca acatcgcaag tccaagagtt
    18001 ataaaaattt ctttcattcg ttccatccct tatttccgat taaaggaaca aattgacagc
    18061 cacctaaatt aattttttct attttatctt ttgtttttac tacaagtatt aaatcttgaa
    18121 tatacttgtc tcccacaggg ataattaatc ttccttcttt ttcactgagt tgttcaagta
    18181 atgtatcagg aatttcagga cttgcggctg ttacaataat tttatcatac ggactgaatt
    18241 cttcccatcc aagtgttcca tcgccaactt ttatctttac attataacca agttttttta
    18301 atgttttttc cgcccttttt gcaagtgctt caatcctttc aaccgaatat acttcacaac
    18361 caatttctgc aagaatagct gcctgataac cacttcctgt tccaatttca agaactttat
    18421 ctttctcttt taattttaat gcttctgaca ttaatgcaac tatgtatggt tgacttattg
    18481 tctgtccttc tccaattggt aatggtgaat cttcataagc atattccttc atattttcag
    18541 gaacaaattc ttctcttgga acttttaaaa aagcatcaat aactttttta tcgctaatgc
    18601 ctctcttaac aatctgagtt tcaaccattt ctcttcttaa attttcaaaa tcttttttca
    18661 tatttcttaa aagatgtaaa aaatattaaa aaaaacattt taaaaaatct tactgtatct
    18721 ctgattggcc taattttact tttttctttt ctgtatatag tttttatggg aagcgatttt
    18781 attcttatcc ctttacaacc tgccttaatt aaaatttctg attctgtttc aaaatgggat
    18841 gtatataact taattttttt gagtgtatct gtttttatta atctaaaacc gcactgagta
    18901 tcaggaatcc actgaaaaga caaaatggaa attaaaaaag acattgaaag atttgtaatt
    18961 cttcttatta aaggcatact ttgagaaaat atacttcttt tgcctataag tattccaatc
    19021 tttgtttttt ttctatattc ttcaacaaat tttaatgttt cttcgggaag atgctgtcca
    19081 tcaccatcca ttgtaattac tgctggaaaa tttttttcaa tagcatattt aaaacctgtt
    19141 tttaatgctt ctccttttcc tttattagtt atatgagaaa ttaaaagtac tttttctttc
    19201 tgtacaattt ctttagttct gtcaatggag ccatcatcaa tgaccagaat atttttaaaa
    19261 tactttttta attcttttaa aattttccca attgcttttt cttcattata tgctggaata
    19321 ataacaagta tatcctctat cattctatta acctttttaa atgatgcatt ttttctaaat
    19381 atggatcaat ttttaaaact ctttcaactt cctttcttgc aagtttaaga atttcaatat
    19441 ctttttctat atcacctatt ttaagaggta atatcccatg ctgtctggtt ccaagtatat
    19501 cgccaggtcc tcttaaatta aaatcaagtt ctgcaatatc aaacccggtt tctttttcaa
    19561 tgaaatgtga aagtttcata tttatttctt catcatcatt ataaactatt aaaaaacagt
    19621 atccaacttc accacttctt ccaactcttc ctcttaattg atgtaattgt gcaagtccaa
    19681 atctttctgc ctgttcaatt attataaagg aagcagaggg tatatctatt cccgattcaa
    19741 taattgtagt tgttacaagt aattttatct ccttattttt aaatttttca agtatttctt
    19801 cctgtttttt cttttctatt ttcccatgta aaagacctga ttttacttcg ggtaaatttt
    19861 cgcatatttc tatatatttc tcttctgctg attcaacatt ctcattttct tttattgcag
    19921 gagttacaac aaatccaatt ttattttgtg atacctgaaa cttaatgaaa gaataaatct
    19981 tttctctgtc cttttttgaa aatagataag ttataacctg tcttttccct tttggtattt
    20041 ctccaagaat ggaaaaatcc atatttccat aaagtgttaa agcaactgtt cttggaattg
    20101 gtgtagcact cattgaaatg tagtgaatat tctcactttt ttcttttaaa acttcctgct
    20161 gttttacacc gaatttatgt tgttcatcaa ctactacaac tttaagattt ttaaaattta
    20221 ttttttcact taaaactgca tgagttccaa ttataacatt tatctttcct gtctctattt
    20281 cttctctaat tttttctttt tccttttctt ttaaacttcc tgttagaaga gaaactgaaa
    20341 tattctgatt tagaaagaaa ccattccagt ttatgtaatg ttgttgagca agtatttcag
    20401 ttggacagag aaaaacaccc tgaaaaccta ttttcgcaaa tatccataat gtaaaaattg
    20461 ctattgtagt tttacctgag ccaacttctc catatattaa tcttcttatg agtttccctt
    20521 tctttatatc atcaattatt tcttccatta tctctttttg gttttttgta agtttgaaag
    20581 gtatgtattt ttcaaattcc tcaataagat ttaattcaat atttatcctt tcttctgttt
    20641 tttgttcaaa gttctttttc cagagtaaat ttaattggaa aataaagaat tctcggaaga
    20701 ttaaataatt tcttgctttt tctaaattta tttcagaaat tgggaaatga atgtttaaaa
    20761 gagcatgttt aatatttgaa ataccgaaca agagtcgcct ttcaagaggt aaaatttcct
    20821 caggaaattc attaatattg ttcaaaaccc attttataat ttttctcata tatttttgtg
    20881 ttatgccttt tgttaatgga taaactggta aaatccagtt aattttcctt ccatcatatt
    20941 cttcataaaa tggatttatc atttcaaatt ctttattaaa atattctact tttccataaa
    21001 agataaaatt ctttccattt tgaaagatgt tttttaaata tatctgattg aaaaaaatga
    21061 tatataaaat tcctgttcca tctggaacgg caattttcaa ataatttgtt tgtccatata
    21121 ttctcttttc ttcttttgca agaacagttg cttttacagt ataaatttca tcctctttca
    21181 aattctttat ttttgttatt ttttttaagt ttatatattt tcgtggaaaa taaaaaagaa
    21241 tatcttctac ttttttaatt ccaagttttt caaaaattat ttctttttgt ttccctattc
    21301 ctttaatctg atttacaggt gagttcaaac tgattttcat ttttcgtcaa atagaaaagc
    21361 atcaagattt tttttataaa aaaacttata gaatttgaga attcggataa tttcaagtgt
    21421 atgggattga tacacttttc tttcatcaat tttatcaatt aatccagata taattgtatt
    21481 aaaaaaaatt acatggtcta taccattttc tttcattatt tttatagatt cttctcttaa
    21541 attttttgtt gaaggtaatc caggtataac aagaattttt ttaaattttt cttttcctaa
    21601 aaaatttttt acttctctat aaaatttttc ttctgttatt tcaaaaattt ctgcagaaaa
    21661 ttttttgatt actgaaggag tgaattttaa agtgtgccat gataaaacct ttattatacc
    21721 ttcatttatg aagaaaattt tttctttttc tattacaaaa ttctcaattt ctttacctgt
    21781 ttcttctgga ttttttacaa tcaaaaatcc atcttttgat attacataaa aattattaat
    21841 tataaaaaac tctttaacaa tttctaatgc tgaaatgctc atattttaat ttatcaaaat
    21901 aatacccata aaataattcc ccttaaaaaa aatagatatt aatgctccaa gagcaagata
    21961 aggtccaaaa ggaacataat catcaatttt tctttttccc agtaggatta atgttaaact
    22021 tattaaagtt ccaaaaagtg aaccaaaaaa taatgttaaa aaaactgatt tccatccaag
    22081 aaaagaacct atcattccaa gtaattttac atctccacca cccatagcat cttttttaaa
    22141 aagcagtttt cctacaagag ccagaaaaat taaaatccca ccacctaaaa tagttccttc
    22201 aagagaatat ataaatcctt ttattttttc catatcatga atctggggga ataaaaaaga
    22261 aattaaaagt ccgattaaaa taccgggaat tactattaca tcagaaataa gaaaagtatc
    22321 aatatcaatg aaacttacaa taataagaca taaagtcaga aaagcataaa ttaaggtaga
    22381 aatagacata ccaaactttt tgtagattag aaaaaacaaa attcctgtta aaagttctac
    22441 tattaaatat cttggagaaa ttgttttttt acagtatcta cattttcctt ttagaaatat
    22501 ataacttatg acaggaatat tgtcatacca ttttatactg ttattacaat tggggcaaaa
    22561 agaaccaggg gagataatag attttccttt tggtaatctg taaatacata cgtttgcaaa
    22621 acttccaaaa ataagtccta atataaaaat tattaatccc atatttttat tttaaatcaa
    22681 aatttgatat tatcaaattt tatttttgca agtaaaaaat ctaatatata attaaaatta
    22741 aaagggaaga tacaatatga aatatgttaa cttaaaagga attaaaatca gtcgttttat
    22801 tataggtggt aatcctttca gtggtttttc tcatcaggga atagaaatgg atatgagaat
    22861 gaaaaaatac tttatttctc aaaggataaa ggaaacatta agagaagcag aacaatgggg
    22921 aattaatact tttataggaa gagcagataa tcacatatta agacttcttc ttgaatacag
    22981 acaggaaggt ggaaaaattc agtggattgc tcaaacatgt ccagaaatta aatctcagga
    23041 attgagtatt gatatggcta taagtggaaa tcctgtggga tgctatattc atggagggta
    23101 tgtagatttc ctttttctta ataatgagat gggaaaaatt tatcctctga tagaaaaaat
    23161 taaaaaatat ggaattttat caggaatagc tggacatatc tctgaagttt ttttatgggc
    23221 agaagaaaat atagatgtgg atttttatat gtgttcttat tataatccag tgaatagaaa
    23281 agaaaaaccg gaaaaagata ttttacagga agagatattt tctgataatg acagagaaaa
    23341 aatgattatg gctataaaaa aacttaaaaa acctgttatt cattataaaa tcatggctgc
    23401 tggtagaaaa aatccagaag aagcaatttc atttatgagt aaaaatgcaa gagaaaatga
    23461 tatggtatgt ataggaattt atcaggaaga aaaaagatgg atgattaaag aaaatataga
    23521 attaatgaga aaatttggat ttctataatt tttgtttttt tttgacttag gaaaaagaaa
    23581 atataaaatt attatatgga aaatatttgg atttcttcat ggaagcattg cgatattttt
    23641 ggtaaagttg atcttgtaat acttgctttt ctttcaattt attcctggta cataattttt
    23701 gaaaaattat ttgtattgaa aaaagcattt aaaagaaata aggattttga aaaaatattt
    23761 ctttcacaga aagaaataaa aagaatacca tgtccacttt attctgttct tgactatctt
    23821 acatcactta ttaaatttga aaagttttct cccgaaataa gtaaaaaata tatagaaaaa
    23881 gcttttatta aagaacagaa caatcttgag aaaaatctta catctcttgc aacaattact
    23941 gcaattgcac cttttcttgg acttcttgga actatatggg gacttttaat tgcatttaca
    24001 aatatgtcaa ttacaggttc ttcttcaatt aaagttgttg caagtggagt tgctgaagca
    24061 cttataacca cagttttagg tttactggta gcaattccag ctgctgttgg gtataatttt
    24121 ttaagggaaa aaatacaact tttgatggat gagatggaat ttttaatacc atatattgaa
    24181 gaatatttaa atgaattatt tttaaaacaa aatgaaaaac agaaataata agaattcact
    24241 tgctcagata aatataacaa atcttgttga cattgcttta actcttgtga taattctttt
    24301 aatgatagca ccgatgattg aacagggaat agaggtaaaa cttccaaaag catctcctta
    24361 tgaaatgaaa attgaaaaaa gtattgtaat tacagtggcg cctgaaaatc aatattttgt
    24421 cggcgggaaa aagatgaatt tgaatgaaat atattcattt ttaaaagaaa aaagtcagga
    24481 aaaaaatatt tctgtgatag taaagggtga tgaaaaagta caatatgaaa atataattaa
    24541 aattcttgac atagcaaaaa aatgcaaaat agatatgatt ggacttgcta cacgaacaga
    24601 gtaaaaaaaa ggagtttttt tgtatctttt ttattccatt tttctatatt tcttttgttt
    24661 tttattccaa tacccgaaaa aaataaaaat tttgaaaaag aaatttttgt tgtaaaactt
    24721 gtaaatatcc ctcatattga agttatagaa aaaacatcag aaaaaaaaga aataaaaatt
    24781 gaagaaagag ttttaccgcc tgtaaaaaaa gaagaaaaaa taaaaacagt gaaagatata
    24841 aaagtagaaa aaaaagtgga attttctgag acattttcac ctgaggaata caaaaagaaa
    24901 ttatattcaa aaatttcaga tacaaagttt tcagaaacca ataaaaaatc ttcaaataat
    24961 acagaaactt caaatataaa tattaaaatt cctgaaataa aatcagtaaa aataagtaca
    25021 ccgagttttg aatctacttc ttcttatcaa acttcttttt ctactgatat tccatcatgg
    25081 tatataaatc tcataaaaaa gaaaatagaa gagaactggt tattgaaaga atatctaata
    25141 gatatgtcaa ctattgtgtc ctttagaatt tatagagaag gaaggattga taatatttct
    25201 atagaaaaga gttcgggata taaaaaattt gataattcaa taatagatgc gataaaatct
    25261 gttaaatcat ggcctgcttt tccttcagaa ataaaagata gatatcttga tgttataatt
    25321 gaatttaaaa cggagggata aaatgaaaaa agttttttta ttttttatga ttttaagttt
    25381 ttatattttc tcccagacac agatattttt tcagattaca aaaaatttag tagaaaaggc
    25441 ggataccttt ttaattttta aagtagaaaa aaatgatttt tcaagtaaaa tgcttgaaac
    25501 cctctctcag gatttaaaat tttcaggata ttttaatgta gaaggaatta aatttaccga
    25561 tgatattgaa aaaacaaaaa aagaaatagt cacacagata attataacag gagaacagaa
    25621 gggagaaata ataaatgtaa aggttgaaga tggacttgag aaaaatcttt tatatgaata
    25681 cagttataaa atttcaaagg acccaaggtt tttagcacat atgatatgcg atgatattgt
    25741 agaaaaatta accggcaaac ctggaattgc aaaaagtaag attcttttta cttctgatag
    25801 aacaggtaaa aagcagatat ttcagattga ttatgatggt tttaatctaa agcaacttac
    25861 aaatgtagag tatcttgtta attatcctcg ttatcttgaa ggaaataaaa ttatttttgt
    25921 ttcttatgaa gatggttggc ccaaacttgc aaaaatggat attttatcag gtaaaattga
    25981 aatatttatt gctcacccag gtttaaatgc ctgtgcctct cctttaaaaa aaacaagaga
    26041 aattgttctt gttttgagta aatcaggtga ccctgaaata tatcttgcgg attttgatgg
    26101 aaagataaaa aaaagattga ccgagaataa aaaaatagat agttctcctt ctttttcgcc
    26161 tgatggtaaa tcaattgctt ttgtttctga tagagatggt aaaccacaga tatacataat
    26221 ggatagagat ggatacggag taagaagaat aagttatatt tcgggttact gtacatctcc
    26281 tgtattttca cctgatggga attttgttgc ttatatttat tcagaaggaa gtacttttgg
    26341 gcttgctata tatgatataa atacaaaaaa aacaagaact tttccgggtt taaattgtga
    26401 agaaatttcc tgggcaccta attcaaggca tattgtttat tcaaaaatag aaagaaaaca
    26461 atctttagta attttagata tttttacaaa agaaacaaga actttaatat ctggaaattt
    26521 taattgtttc agtccaaact ggtttcttat tgaataaaat tgaaaggata tatatttggt
    26581 ttaatctctg ttttttcatg gggaactgta tttgtaataa gtagattttt aatccatggt
    26641 gaagttatag accccttggt tcttgctttc tggcgatttt tctgggcttc tttttttctc
    26701 atgtttttta taaaatttaa ttttaaaaaa gtttttagaa tttttaaaaa agatgttttt
    26761 agatttctga ttcttgctct ttctggaatt tatttgatgt caacttttat ttttatatca
    26821 gttaaaacat cttctgcaac gatttcaagt attttaatga gttctaatcc aatatttgtt
    26881 attcttattt ccattctttt tttgaaggat aaaataaccc ctgaaaaaat tttaggagtt
    26941 tttattggat ttattggatg tattattgta ataaaagagg attttaaaat ttttttttgg
    27001 gctggagaga atataaatat ctattcatta cttgctgcga tttgttgggc tgtttataca
    27061 gtaatagggg aagaaccaac caaaaaatat ggtgctattg aaacaacctt tattgcttct
    27121 ttcattggaa caattttttt atttttaacg gttaaattac tcaacttaaa tcttttgggg
    27181 aagaatattt atggtttttt atctggaatt tatctcggga tagtaccaac tggtattggt
    27241 tttacacttt ggttttctgc tcttaactat ttaaggcctt ttttacttgc tccttttcaa
    27301 tatttaacac caattataac ttattttctt tcaattttaa ttttgaaaga aaaagtatcg
    27361 ttaattgttt ttatgggtat gtttctgatt tttttctcac tttttttaat tcaaaaaaag
    27421 gaaaattgaa attgttcttg acttaattta aaataaaaag taacataact taaaggagga
    27481 ttgaaaaatg tatatttttt tattaatatt gcatattctg gtttctttat ttttgatatt
    27541 tgctgttttg attcaaagtg gtaaaggttc atcaatggca aatatttttg gaggaggagg
    27601 aatggaaaat gtttttggtg ctgaaacacc ggctatttta aataaaatta catcttttct
    27661 ggctgttatg tttattatta cttctgtatt gcttgcaata attcctggca aactgaccac
    27721 taaaagtatc cttaaagaag aattaaatag acccggaaag attcttcctg taaatccttc
    27781 cactcctcct aaaaattaat atataaaatg aggaaattag ttgaactacc taaaaagggt
    27841 aaacttgtaa ttattggtga tacacatggt gattttaatt caacaagaat agttgtaaag
    27901 aattttattg ataaaaaaaa ctattacctt ctttttcttg gtgattatgt tgatagaggt
    27961 ccaaattcaa aagaaaatat tgattatctc ctttcactta aagagaaaaa agaaaattta
    28021 attttgcttc agggaaatca tgaaatgtat cctgtggttg aatgcagtcc ttcagatttc
    28081 tgggacaatt taagtgaaga cgaatttaat ttttacaaag aggaatttat taatttacct
    28141 tttgttgctt atggaaatgg ttttattgct ttacatggtg cattacccga tattgaaaaa
    28201 ttagaggata tagaaaaaat agaaattgga gataaagaat ggtttaaaat tgtatggggt
    28261 gattttaagg ataaagaagg agagtatctt ggggactttc ttggaagacc aaaatttgga
    28321 aaggactatt ttttaaaaat tatggaaaaa attggtttaa atgttcttat aagaagtcat
    28381 gacccttatg cacctgaaag aatgtttgat aataaatgtc tgacagtttt tacctcaatt
    28441 gcttatggaa ccgaaaggaa aattgctttg gtaaatctga aaaaagaaat aaaaagtatt
    28501 gacgatattg aattaatttc actggataaa ccagaggttt aatatgagaa taaaaaattt
    28561 tggtggttat tctctgggtg aatatcttgg aggtggttta tatacaaaag tttataaagc
    28621 atattcattt ttagcaaaac caacttatgg aaatcctgtt gctataaaaa tactttattt
    28681 aaaaggaggg attgtagaaa gaaataaact tataaaacaa tttgaaagag aagcagaaat
    28741 agcaatgaat ttagaacatc caaatatagt taaggtttat aattttggta gatttgacca
    28801 tcactatgca atgattatgg aatatattga tggaaagaat ttaaaagaat tcttgtatga
    28861 aaagggaaaa tgggatttaa ataaaatatt aagaatttgt tatgaagcgg gaaaaggact
    28921 tgagtatata catcagcata atatagttca taaagatgta aaacctgaca atattcttat
    28981 ttcttttgac tttgaaaaaa ttaaatttac tgattttgga attgcaaaac tcccaaaaaa
    29041 atggtggcac agagatatat ttcctaaatc ggggactata acaaaatttg gtataatttc
    29101 atatatagca ccagaacaaa tgaaaggtaa tgcaactttt cagtcagata tttatagttt
    29161 tggagttact atggatgaaa taataacttc aaaacttttt atggagggaa aagaagatga
    29221 ggactacttt aaaagaatag acattagagc acaaaggaaa aacacaggaa agcaaagaat
    29281 tttatgtgag gatatgccgg taccatctga attaaaaaat attattaaaa aagcaactga
    29341 aaatgagata aatttgaggt atcaaagtat gacctatctt ttagaagatt tgaaaaaatt
    29401 aatttaaaat gagaaaaatt tcaactttaa ttccaattct tttaattttc accgctggtt
    29461 gtattgagaa aaaacagatt aaatttagaa atactctctg tctttccaca tcatctgacc
    29521 caaaatcatt taatcctatt attgcgaaag aaacaagtac aacaataatt actggtttta
    29581 tttttgaagg acttacaaag atagatggaa taacactcca ggttaaaccg ttacttgcag
    29641 aaaaatggga agtagataaa acaggcaaaa tatggagatt ctatttaaga aaagatgttt
    29701 tatggaatga cggagataaa tttactgcgg atgatgttgt ctttacattt aatcaactta
    29761 tttataatga aaaaattccg acaagtagta gagatgttct tactgtagaa ggaaagaaaa
    29821 tagaagtaaa aaagattaat gactatactg ttgaatttat tttgcccgaa aaatttgctc
    29881 cttttttaca acttatgaca caggaaattt taccgaaaca taaacttgaa agatcggttt
    29941 tggaaggaaa atttacgagt acatggggag tgaatgaaaa agtagaaaat attgttggaa
    30001 caggacctta taaaataaat gactacagac ctggtgaatg gataattctt gaaaaaaatc
    30061 catattactg gagaaaagat aaaaatggtg ttcaacttcc ttatattgaa aaaattgttt
    30121 ttttaatcat tcctgatccg aatatggcta ttttaaaatt tagaacaggt gaaatagata
    30181 tactttcaat tagaggtcag gattattatg ttttaaaacc acttgaaaaa gataaaaatt
    30241 ttacaatata tgatattggt ccttctctta gttctgaatt tcttgcttta aatcaaaata
    30301 ttgatgcgaa aatacctgat tataaaaaag aatggttttc tgatgtaaat ttcaggaaga
    30361 gcatcgctta ttcaattgat agaaattcaa ttattaaaaa tgtttatgct ggttttggaa
    30421 ctcctttata cggaccaatg aataaatcct gtggattttt ctataatgat aaaataacaa
    30481 aatatgatta tgatttaaaa aaagcaagag aattgttgat gaaaagtgga tttgtttgga
    30541 aagaaaattt tctttatgat agaaaaggta atagagttga gtttacaata ataacaaata
    30601 gtaataattt tgagagagtc caaattggca atattatcca gaatgacctt gaaaaattgg
    30661 gaatgaaagt aaatttactt cctgttgaat tcaataccct tgttaataaa ctcacaattt
    30721 caagggactg ggaaggagta ataattggtc ttacaggagg aattgaacca cacgggggga
    30781 aaaatgtatg gaattcaaaa gggcaattac atttctggaa ttttggtaat aaaagaaatt
    30841 atgaatggga aaaggaaata gaccatttat ttgaaatagg tacaaaatat ttaaatcagg
    30901 aagaaagaaa aaaaatatat gataggtggc aggaaatagt aactgattat ttaccactta
    30961 tatatacagc gaattcaaat gtaatttatg ctgttagaaa taaatttgaa aatatgaaaa
    31021 ttactgttta cggaggagtt ttacataata tagaagaaat aaagataaaa gaaaatggaa
    31081 agttttagat taagagtttt ttataaaaag gaaggtattt caagatttat atcccattta
    31141 agtttttgta aattaattga aagaagtttg aggaggttag aattgccctt aaaattttct
    31201 gagggtttta ccccacatct taaaatttct ttttgtccgc ctttacctat tcccatttct
    31261 ggaaataacg aattttttga aatagaggtt tatgagaaat ttaatttaga atcttttgtt
    31321 aaagatataa atttaatttt acctgaagga acagtggtta aaagatgtta ctggataagt
    31381 cataagtttt ctatatcttc aatttatggc acatactcaa ttcctttaaa agaacctata
    31441 attaaagaaa aagcagaaga atttggtaaa ataattgaag aaaaagaaga gtttattaag
    31501 gtaattttta aaatggagaa tttcagtcat aaaaagattt ttgtgaatgg aatttttgat
    31561 gggataacaa gagaattatt tattgaaaat gaacaaatat agaattttta taagcaatga
    31621 aaaggttatt ttgagagtat taatagagaa agaaggtaag atagataaca tttttataca
    31681 caaatatgat tttgaacctg atataggcaa tatatataaa ggaagggttg aaaaattttt
    31741 atatggattg aattctgctt ttgttaatat aggtgaaatg aaaagtggtt ttttacagtt
    31801 gaataagtca gaattttatt tttctttgga agaaaatgaa gatatttcag gtcatcaaat
    31861 tttaaaggaa gggaaagaag ttcttgttca gatttgtaaa cctggtgagg atgagaaatc
    31921 accaaaagtt actgaaaagg tagtactgcc tggtagattt tttgttttaa ttcccaattt
    31981 gagaatccaa aaaatttcta aaaaaatcga agatattagg gaaaaaacaa gattaatgaa
    32041 gatttttaaa aaaacacttg gtaaaaaatt tggttttatt ataagaactg cagcattaaa
    32101 taaaaaagaa ttttatattt atcgtgaaat agagcatatc ttgaatatat ggaaaagaat
    32161 aaagagggat tataaaagaa aaaagacacc atcattatta tggaaggaac ttccattgta
    32221 tttaaaagtt ttaagagatt atacagatga aaattgtgaa tttgttgaga tagatgatga
    32281 aaatatttac agagaagttg ttaaatatgt aaatttattt attcctgaat taaaaggaaa
    32341 aatttatttt tatagaaata aaattccaat gtttgtaaaa tatggatttg aagaaaaatt
    32401 tgaaagtttt atttcaaaaa ctgttttgct tccatcaggt ggatacttga ttatagagaa
    32461 gggaaaaact ctaacagcaa tagatgtaaa ttcaggtaaa atggaaaaag aagattttga
    32521 ggaaacaatt tttaaaacaa atatggaagc agcagaggaa attccaagac agattagatg
    32581 cagaaattta tcaggattga ttattgttga ttttattgat atgaagggaa aatttaaaga
    32641 gaaagtattt aaaaaattta tagaaaatat aaaaggcgat aaatcaagaa ttaacatttt
    32701 atccatttct aaacttgggc ttgttgaaat ggcaagagag aagaataatt attcaatcta
    32761 taatttatta ctaaaagaat gtcctttttg tgacggttca ggtattgaaa aaggtaaaga
    32821 aattgtatat atccagttga ggaaaaaaat ttttgaaatt ttttcaaata aggcatataa
    32881 tggtaaactt gaaattgagg tttcacaggg gttatttgaa tttattttta agaatcaatt
    32941 atttaaaaat tttttatatt ctgatagaat aaaatttaaa gttaatggta aacttaaaga
    33001 agatgaattt aaaatattta tatagataga aggaggaata aaaatggcta tgcatgtgaa
    33061 aattgaagaa aaagaaggtt atcaaaaaat tttaactcct gaaaattctg atttgaaact
    33121 tttatctttt tctattttaa gacagaaaga aggaattttt gaaggcaata ctgaaaaaga
    33181 agaaacagtt tttgttatta cagaaggaaa atgtaatttt tatattgaga aaaatcttat
    33241 agggactatg aaaagaaaaa atgtttttga ggaacctcct gttgcagttt accttccgcc
    33301 ttattataaa tataagatag agtttgagaa aaaaacagaa atttgtattg ttggatgtca
    33361 gggtaagggt actggaaaac caaaattttt aaattcaaag gaaattaaat ttagaagggt
    33421 aggagaagaa acatatttca gaaacattac tgatattata acagatgcct ttccttccga
    33481 aaaattactt cttggagaga caataaatga ccctgggaac tggtcaagtt atccaccaca
    33541 taaacatgat aaaaataatc ctccagaaga atattgtctt gaagaactat atttttttaa
    33601 aattcatcct cagaaggggt tcggtgttat aagaattttt gatgagaatg aggacaatct
    33661 ctttttaatt caaaataatg aggttgttac aattccaaaa ggatatcatc ctgttggagt
    33721 tattccaaaa catcagatat attacttatg ggcacttgca ggagaaaaaa gaatattaaa
    33781 accgaaagtt catcctgatt ttaccttgta aaaaggagaa aaaaatggtt ccaaaaaaag
    33841 tttttttgac aaaaggagtt gggaaagata aagaaagatt gacaagtttt gagaaagcac
    33901 tcagagatgc agggattgct cagtttaatc ttgtttcaat ttcaagtatt ttcccccctg
    33961 gttgtaagtt aattcctaaa aaagaaggat tgaagttgtt aaaacctggg caaattgttt
    34021 atgttgttat gagcagaaac tcaactaatg agccacatcg tcttataact gcttctgttg
    34081 gtatgacaat tccaaaagac ccgaatcaat atggatattt atctgaacat gaaggatttg
    34141 gagagacaga agaaaaagca ggtgattatg ctgaagacct tgcggcaaca atgctggcaa
    34201 caattttagg tcttgaattt gaccctatgt catcatatga tgaaaaaaaa gaaatatgga
    34261 aaatgtctgg aagaattgtt aaaacaacaa atattactca atcagcaatt ggtgataaaa
    34321 atggtctctg gacaacagtg gttgcggctg ctgtttttat tttataaaaa gttttgaaga
    34381 gaaaaaaaat ggaaaaggat tatcaaatta taagaaaact tgctgaaaaa gtaaaagaaa
    34441 tagcaaatca tccagttcag aaggaaaaag cagaaatatg gaaaaaacat aataaacttg
    34501 aaagagtaag acctcttgtt ctaatttttc ctgaaggtag ttggagagaa attttgcctg
    34561 aaaatgaaat tttatgtgag gatacttttt taagaagaat tgaatgggat ttaaggcaga
    34621 gaatttatta ctgggaaaaa attaaagatg ataatgtgat agatggtaaa gtttattctc
    34681 ctattgtcat aaaaaatact ggatttggtg ttgaagtaaa agatataaga cctgaatttg
    34741 cttatggagc aagacatttt gaaccagtta tcaaagaaga aaaagacata gaaaaattaa
    34801 aaaaaccaga gatttcaatt gactgggaag aaacagaaag attatataag aaaacatgtg
    34861 aggcagttgg tggtattctt gaagtagaaa aaagaggtcc ttctcatcca tggtttgctc
    34921 ctattgactg gtttattaca gtaagaggga ttgataattt ttttatggat atggtagata
    34981 gaccagaatg gcttcataga attttaaact ttataactga atgcaagata gaagaatggg
    35041 aattttatga aaaaaacaat gttttatcac ttaataatgg acctcactat gttggttctg
    35101 gtggagttgg ttttacagac caattaccac aaccagattt tgacggaata catgtaagaa
    35161 ttaaagacct ttggggacat gcaacaaccc aaattttttc tcttgtttct ccacagatgc
    35221 atgaggaatt tgctttaaaa tatgaaggaa gatttttgag taaatttgga tttaactgtt
    35281 atggatgctg tgagccattg cataaaaaaa tagatattat tttcaaacat ataccaagat
    35341 taagacgtct ttcaatgagt ccctgggttg atattggaga agcagcagaa aaacttggga
    35401 aaagagcaat tttttcatgg aaaccaaatc catcttttat ggcttcagat acatgggaag
    35461 atgattacat aagaaaaaca attacagatg catgtgaaaa aacaaaaaat tgtgttattg
    35521 aaataatact taaggatata catacttgca gaaataaacc agaaagaata tcaaactggg
    35581 ttaaaattgc aaaagaaata gcggaaaaat attaaatgga aaataaaaag aaaatctcaa
    35641 tatgggaaga taaaatacct tattcaaaag gaaattcaga ggaagatata ccaaaattga
    35701 ctccttattt aattgaaaat aaaaaaaata atccatgtat aattgttttc cctggaggtg
    35761 gatattctgt tagggcgcct catgaaaagg aacctattgc tttatggtta aatagaatag
    35821 gaatatctgc atttgttctt gattatcgtg tttctcctta cagatatcct gttccctttc
    35881 ttgatgcgca gagaagtata agatttgtaa gatataatgc tgaaaaattt aatatagacc
    35941 caaatcgtat aggaataatt ggtttttctg caggaggaca tcttgcttct cttacaggag
    36001 ttcattttga ttatggaaaa aaagagaatg acccgattga aaaaatttct ttaagacctg
    36061 atttattaat tctgtgttat cctgttattt catttttaaa ttacagccat cagggttgta
    36121 taaaaaattt tcttggagaa aatatagata aagaaattct tgaattttta tcatctgaaa
    36181 aacaggtcaa gaaagatact ccaccgtgtt ttatttggca tactcagaat gatgatgttg
    36241 ttcctgttca gcacagtatt ttatttgctc tatcccttaa agaaaaaaat attgattttg
    36301 aacttcatat ttttgaaaaa ggacttcatg ggcttggact tgcagaaaat gatttttcag
    36361 tttcttactg gactatttta tgtgaaaaat ggttaaggaa acataatttt taaaaggagt
    36421 taaaaatgaa gatagcgata atcggagcgg gaagtttagg atttacaaaa agatttatca
    36481 ttgatattat aagtgttcca gaattaagaa attttacact tgctttaaat gatattgatg
    36541 aggaaagatt gaatttaata acaaaatggg caaatgcttt aaaaaaacag gaaaatttaa
    36601 aaattgaaat tttatcttca actgatagaa aagatgttat taaaaattct gactatatta
    36661 taaatacaat cagagtagga ggacttgaag catttgaact tgatgtttct attccgagga
    36721 aatatggggt tcatcaggca gtcggagata cagttggtcc aggtggtgta ttttatgctt
    36781 taagaacaat tccagtttta cttgaaatat gtgaggatat taaaaaatta tgtccggatg
    36841 catggttttt gaattataca aatccaatgg caatgaatac atgggcaatg ataaaatacg
    36901 gacataaaaa agttgttgga ctttgtcaca gtattcaggg aactgcctgg caattatcaa
    36961 gttatatagg agtaccctat gaagaagttt attactgggc agcaggaata aatcatatgt
    37021 catggtttct tgaatttaaa agaaatggaa aagaccttta tcctgaatta tttgaagcaa
    37081 tgaaaaaccc tgaaattttc aaaaaagacc ctgtgagatt tgaaataatg aaacattttg
    37141 gatattttgt aagtgagtca agttttcata tggctgaata tgttccatat ttcaacaaaa
    37201 agaaagaact tataaataaa ttcaatacgc atggatggat atatcttgat gtttgtaaga
    37261 atgcttatga ccctgcaact gaaaaattaa aaaaagaggc agagggtaaa gaaaaaattg
    37321 aaatatcaag gagtaacgaa tattgttcat atataatcca tgcacttgaa actggatgta
    37381 ttttaagatt taacggaaat gttttgaatg atgaaataat agataattta ccttatggat
    37441 gctgtgttga agttccatgc tatgcagaaa aaggaaatat aaggcctgca aaagttggaa
    37501 atttaccaat ccagtgtgct tctttaaaca gattaaatat aaatgttcag gaaattactg
    37561 ttgaggcagc aattaaaaga aaaaaatcac ttgtatatca agcagttgca cttgaccctt
    37621 taacaggagc agtattatca cttgaagaaa taaaaaatat ggttgatgaa atgtttgaaa
    37681 aagaaaagga atatttacca gaatttactg attaattgaa aatgtataaa acagggattg
    37741 gttttgatat tcataagttt gctgaaggaa gaaaatttat aattggaggt attgaaattc
    37801 cttatgaaaa agggcttatt ggacatagtg atggagatgt tttaattcat tcaataagtg
    37861 atgcaatact tggagcactt ggaagacccg atattggata ttactttcca gatactgatg
    37921 aaaaaataaa aggaatagat agtaaagaga tattaaaaag agtgattgga tttttaaaag
    37981 atgatagata tgaaatagtt aatattgata gtgtgattat atgtgaaaag ccaaaaatcc
    38041 ttccatattc tgaaaaaata aaaaaatccc tttctgaagt aattggtata gaaaaagaaa
    38101 aaataaatat aaaaggaaaa acaacagaag ggattgataa aggtaattac tgtgcttgta
    38161 tttcaattgt attgattgaa aggttctgag ttaagagatt gtatttaggg ttaaggtaga
    38221 aatttttgaa ataagaattt aaga
//
LOCUS       DAOSAT010000005        37908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_809_length_37908_cov_5.343724, whole genome shotgun sequence.
ACCESSION   DAOSAT010000005 DAOSAT010000000
VERSION     DAOSAT010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 37908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37908
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_809_length_37908_cov_5.343724"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1031)
                     /gene="cas6"
                     /locus_tag="PLF90_00825"
     CDS             complement(105..1031)
                     /gene="cas6"
                     /locus_tag="PLF90_00825"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HOK55860.1"
                     /translation="MDFEFIKNLKFIIISFRIQPDKTLTLPFFKGATIRGGLGYSFKK
                     NVCVKNETDCKNCSSFSDCVYVNLFEPKKIKEKIEEIPRPYVIEFPVNKKNVYREDEI
                     FEFNLILFEKAVKYFPYFLISFAYLGEKGIGKFREKFFIKEVFQKYPVEKEIFDFRKG
                     TVEMPEVREYELIEKDIDKISIKFLTPTKIKSQGDFITVPEFEVFIKAVLRRISQILY
                     FWCDFKEKIDYSEIIKEAEKVKIGKCNLYWKDFKRYSTRQKEEMKLGGILGEIEYRGD
                     IGKFYPLIEIGSYIHIGKNTSFGLGKYIILTK"
     gene            1214..2326
                     /locus_tag="PLF90_00830"
     CDS             1214..2326
                     /locus_tag="PLF90_00830"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOK55861.1"
                     /translation="MKKIVFFYFLVITLIFSQEIPQKIKDILGKTESILKDKMIESRI
                     ESSYKMMGMTTLFDIRIITDLKTEKFYMEMGEMGITVYDGENLWTYNKMSNLYTKIPI
                     SKDELEKNKESFIPTSIKEISEFLPKLDSSSIEEVNFDDKKCYRIELTGKDKRNNNVK
                     LTLFIDSSNYLPAQFEIESKFPSINEPIIIKNRIKTLKINPEVPSDIFVFKPPSGAKE
                     FKFSDTRTSKEESLEGKKAPDFNLKDIEGKSITLSSYEGKVVLLNFWASWCPPCREEL
                     KVIQKIYNEYKDKGVIVLCVNSGEDKEKVEDFIDENNYKFPVLLDTDSKVSDNYKVNT
                     IPRVLLINKDGIVVKDLTGYTSENEKILREEIEKIK"
     gene            2362..3081
                     /locus_tag="PLF90_00835"
     CDS             2362..3081
                     /locus_tag="PLF90_00835"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HOK55862.1"
                     /translation="MNLYSVKINPVKKILGIHILLAVLVFAQINFPPKIKEVLNKTEN
                     IFKGKKIEAKYIFSTTIGKYLDSDSKQKNYEIIEIESNLMADLNSGKFYLENGDRQIS
                     VYDGNYLWNYDKVINAYNKIPLKNTPSHFVFPLFIIDLAKAIPKIESVDMEEVSFENK
                     NCFLIKFSGKDFANNSCTVNLWINKENYYPVQFQSLTTIGTELSEMNYKFKSLKINPE
                     ISDDKFNFKIPEGAKEIKTGK"
     gene            3096..4466
                     /locus_tag="PLF90_00840"
     CDS             3096..4466
                     /locus_tag="PLF90_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55863.1"
                     /translation="MKKIILFLIFVNFLFAQSKKDVKLVIYNQNFAVCDEIREIEVPA
                     GKNELIFTDIPRFIEPVSIQISVLSPSIKFNVLEQSFNYDVYTSDKVFAKNIGEKVSM
                     ITKDGQFYSGILLFYDAGNIGIETKDKIIFVNKENLREVSFGKMSTFTYKPQLKFLVQ
                     NEKKDIYKILLKYTTSNINWRADYLGSFDEEKSVLSLTGFITVNNKCGIDYENASIEL
                     VAGEVKKIAEIENQRKAFGAADALKAEMAGAPSFEESPVFEYHRYTLKGKTDIKDGEI
                     KQINFVNKNDLKVEKKYKYDGAITRYYHYDNWRNLNYNDKVEIWIEFKNEGEILPAGK
                     MKIFKNEKEGSIFIGENRIPHTPVGEKVRLSLGNVFDIKGERKIIAHERISQQVYKDT
                     YEIKIKNFKKEDVSVEVIEHLYGSWEITEKSHNYEKEDAYTIKFPVKVKGSGEEIIRY
                     TVITRF"
     gene            4478..5587
                     /locus_tag="PLF90_00845"
     CDS             4478..5587
                     /locus_tag="PLF90_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55864.1"
                     /translation="MKKVLIILFIIFSISYARTKLVALPDREISYIRFDAPYTAFVEE
                     ERILTLQKGINQVDFSWKGVNIDQDSINLTFLTNPDKLKLISLSYPPEGNSLIWEIYS
                     PEGGEEKVRISYLLSNIDCLYTYKIVVDKNEENINFTSYIILRNFSGEDFEKGIVTPE
                     FGKTFETSTKNEETKQILIYQKDGIKFEKIFVFDASRMIWDPETAKENVGIPVYFEIK
                     NVKESGLGEFPLLLGKTRMFQIESSGSSIFLGEDNLKYIPVGESFRLYFGDSRDIVVK
                     QKKMKDAKINIRKNNSGDIVLYDTDEIIRIEIENFRDKIAKIEILENISDEWDMEEST
                     HKYEKIDSNNIKFVIDLKPKSKEIVEFHYHRRNLR"
     gene            5584..6891
                     /locus_tag="PLF90_00850"
     CDS             5584..6891
                     /locus_tag="PLF90_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOK55865.1"
                     /translation="MKNKIFLIFLIFVLNLFAKVDLVTLPSREKVQLTIYNSADLTLV
                     RDERILTLKKGENSLQFSWANTLIDPTSLDLIPKQDVASINISQLIFPPRVSELGLWK
                     IESKFSKKIPFEISYFTSGISWEAFYIGTLSNDEKSMELKGYVKVINKSGEDYENASV
                     RLVVGKINLLEMIRVLAKRYPPYGTPGYLPPAPLIEKERGVEMLKAAKAVLAEAKEKE
                     IIKEGVSEYFLYTIEGTENLPDKWSKRLLSFSQKDIPVEFFYKYEEERYGKNARRFIK
                     FANDQNHKLGKEPLPDGLIKIFKYTNKEGNLSYMGSDKTKYIPIGEKVELDLGESKEV
                     KIDTKLMDLKKKNFVFNKDGNISGWEEEKIYKIKVTNYKNLPVKVEIKRNFPHQYWKI
                     EVDKNIQWEKYDIGSVKFTLTLAPFETREFTYKLILFEGDRRY"
     gene            complement(6950..7906)
                     /locus_tag="PLF90_00855"
     CDS             complement(6950..7906)
                     /locus_tag="PLF90_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55866.1"
                     /translation="MGFLKLNKENFKPLSFQYHKILSQNFSLNSIVNYKDDIFIASNS
                     GIYVFPSERPLYIMNEENGLVCENIKTLEILNDKLYAIAEGSVKGLIEIDLKTGISKI
                     IFSKKQEENKKGIEGKTIAGIKSDKEEKIFWIVVGKRDPLICSAEDETAIFKYNPETG
                     KYEKFKYSYQKTEDWLNFAGLLDFDDFKKNKNHLILSVFPKMIVQKDNSLSEKYWICD
                     IFDFDIKNRNLKRYIIKSPSEEESLWNEKKQPFTIIPLENKYLVVSKQNMIIFEQESG
                     NVEVIWQNKTDKTKYINDIILTEKGLFVLTSDTLYLIPEIKN"
     gene            complement(8109..8345)
                     /locus_tag="PLF90_00860"
     CDS             complement(8109..8345)
                     /locus_tag="PLF90_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55867.1"
                     /translation="MIIESYIELIVWAHKNNKYKNYSYDNWQQLLITNITSYLTSSGL
                     IENNKDNKEIIKEVLEKNIFKPQDNQYFLNSSLL"
     gene            complement(8348..9289)
                     /locus_tag="PLF90_00865"
     CDS             complement(8348..9289)
                     /locus_tag="PLF90_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55868.1"
                     /translation="MELRETEKALSLIESAVAISPDSPDYNSKLLLLLTYKLGEKKDA
                     QEKLKMFLKCIKLIDKFFEQVNKDKKVFYIWKNNLISFLLSITDSLPTTKELKYDSEA
                     QQIIKKVHENFWELIDFYKDIVLNDPFYLAKNKNFEKMRTMELYTILRVANYGIRFCP
                     DIKVAIEKSREILSLCIEKKIIIPDITTEFWVIGLEGIVRPVIFKKYKDVEWLDEKNI
                     SQVEKEIEKYLSELHEGDNILIKLSKERVQIFFPWEIEKNLEKLKTHYRKYFEILKDE
                     NLEKLPNFNVVEFLFHTGCEIVRYFLVNRRMKFIKKF"
     gene            complement(9280..10107)
                     /locus_tag="PLF90_00870"
     CDS             complement(9280..10107)
                     /locus_tag="PLF90_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55869.1"
                     /translation="MFKSKILFLILFFIFSFFFFSYSDNIKLAIIDIGKDKVPKELFD
                     FIYFSFNNYPEIALIEREEIEKIFKEQQISISFSGENVIEVGKLLFADSFLMIELRQK
                     EKKNYIRFQLVETHYGIKLWSSSMLLPDSKGEYGKIAEDISKVVSQRIKRIKIPIEEL
                     ILLGLTEIRSEEITRKWDYIGDELKINIEEILSLYPEVILLERKRTGILMEERDIVKG
                     LPEKLISSAIYIDGSYRINREKKEYIVEVYIRGRQNEKKIFEIKEEGEINKLSDIWN"
     gene            complement(10208..11050)
                     /locus_tag="PLF90_00875"
     CDS             complement(10208..11050)
                     /locus_tag="PLF90_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOK55870.1"
                     /translation="MKKVLISYFIISFLFIVGFSYSEVKSNQFISVAVSDFESTIPKM
                     ERIGSQVSSIISANLSLDERIILVERQDLNRILEEMQLGMTGTISSDSAVKIGNLTGA
                     KVLITGRVFEDKNKLYIAAKIIGVETGRVYTEMIVFPLEDSLNDEVVKLSAKIRNVIL
                     QKTDTLMVKEKKKEDVINSIKKLIEGKKLTVVAVEVRESHLRQLVVDPAVETELVYIL
                     KELGFEIVDRNKSEKKPEVLITGEAFSEFGTRKGGLAICKGRVEIKAIKVDNGQILTV
                     ERQK"
     gene            complement(11031..11252)
                     /locus_tag="PLF90_00880"
     CDS             complement(11031..11252)
                     /locus_tag="PLF90_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55871.1"
                     /translation="MPSGTLIIVFHTENIEIPYIKIEENHGLRIDVKMPIIDNITSPE
                     YQELFIKIFNIALENLNIKKEVFYEEGLN"
     gene            complement(11612..11767)
                     /locus_tag="PLF90_00885"
     CDS             complement(11612..11767)
                     /locus_tag="PLF90_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55872.1"
                     /translation="MKKVYCVFGGQKVILNLIVYGKIPEKVNINADIFQIARNTALPL
                     KRTSLLQ"
     gene            complement(12005..12388)
                     /locus_tag="PLF90_00890"
     CDS             complement(12005..12388)
                     /locus_tag="PLF90_00890"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HOK55873.1"
                     /translation="MRKKKKYNFKDLLKIMEKIRENCPWDKKQTNESILKYLKEETEE
                     FTNEVNKKNYDGMKEELGDILWQVVFHSQIMKEKDIFTIDEVIDYLCKKMIKRHPHVF
                     GNKKVKDEKEVIENWEKLKQKEKSH"
     gene            complement(12390..13223)
                     /locus_tag="PLF90_00895"
     CDS             complement(12390..13223)
                     /locus_tag="PLF90_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOK55874.1"
                     /translation="MGKYFPEKICYFIAYILTELRYFFTPSLRKIMKKNLKIILEFKE
                     KRNLSEKELNKYVKGVYINFGRYMTEFFTIPKLNYEKVKEKVIVENIEVLDSALKKGR
                     GVIALTAHIGNWELAGVVTSILGYKICAIAIPYMTPKITEIYRRIRESKGVEVIFTGS
                     NPKDFIRFKRENKVLAILGDRVFTEKGEVVNFMGKKAIFPRGPATLAVKLKTEFISGF
                     FVRENDRYKLFFEEINYPPENLSEEEKIDYLLNESVKKIEKVILKYPMQWLSFQNIWA
                     E"
     gene            complement(13262..13855)
                     /gene="recR"
                     /locus_tag="PLF90_00900"
     CDS             complement(13262..13855)
                     /gene="recR"
                     /locus_tag="PLF90_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOK55875.1"
                     /translation="MGLYPEIIETLIEKLKKLPGIGPKSAERIVYYLIQSDDNEVISL
                     GETIISLKKQIKLCKRCFNFAEKELCNICLDEKRENILCIVEEVKDLISIEKTNFKGR
                     YHVLWGRISMLDNITPEQLKIPQLIERIKSENIQEIIIATNPTVEGDNTAEYISEILK
                     KLGIKHSRLAIGLPLGSELEYIDSQTLKKAIEGRREL"
     gene            complement(13858..14151)
                     /locus_tag="PLF90_00905"
     CDS             complement(13858..14151)
                     /locus_tag="PLF90_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOK55876.1"
                     /translation="MGIFDNLKQMAQLKQQASQFQKMLESKIVEVSSSGNEVKIKING
                     KMDILSIEISETLLKPENKVNLEKLIKKTFNEARGKVEKIIASELKSQIGFPF"
     gene            complement(14153..15769)
                     /gene="dnaX"
                     /locus_tag="PLF90_00910"
     CDS             complement(14153..15769)
                     /gene="dnaX"
                     /locus_tag="PLF90_00910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOK55877.1"
                     /translation="MSIKVESGKIIFKMYKVLARKYRPQNFDEVCGQKDIVEVIKRSI
                     KFNKVAHAYLFAGPRGVGKTTIARIFAKCLNCVEGPALNPCNKCDSCIEITNSASLDV
                     LEIDGASNRGIDEIRNIRENVNLLPAKSRFKIYIIDEVHMLTPEAFNALLKTLEEPPP
                     YIKFFFATTAPEKIPLTIISRCQRFNLKPLKKDEVKNKIMEICGKENIEIEEKAIDEI
                     YDFCEGSLRDAISLLEQLSVFSDEKIKHQDVRYLLGLPEEKSIEEILKNILDKNYTKS
                     IENLHKLISDGKDPVLILEGIIKKVKDIVISKLDIEKIEIDEQLVKRFNNLKTEEIFE
                     GIEYIVEFKSILRREREPVLFTEVLILKLLQLWGNNKKELKEEIKIEKKEENKIKKEE
                     EITDEKKENMGTIFDFKEEKETEKNEKEEGQKIEEKKENKEFNWEEILKKVKHFRPTL
                     EAALREGKLDRIEGENIYILFDSKYNFHKSMVENISNKIKIEKIIFDLTGKKYKIIPI
                     LNKNSKSIIENPEVKKIIEFFNGEIIKMEE"
     gene            complement(15773..16933)
                     /locus_tag="PLF90_00915"
     CDS             complement(15773..16933)
                     /locus_tag="PLF90_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55878.1"
                     /translation="MENLEKELEKRKWPKRPEIHINFKKYNSYVTYGRVENRSLRLFQ
                     SLSEPIPPEECAITSLCKGKNRKIYGATSGKRSHLFYYSFSPEGDGVCDISIIEGAKS
                     VKNSLCVDKTGKIWGGVSEGEKGYIFCYDTSGDHMGEGGTFSGEIKIISEPVPGEFIV
                     SLICDEDKNVLYGISSESGYFFIYDITKNEVSIKGQIDKNKLFSSVLVKDNKGNVYFF
                     GQLCQMFKYSPEEDKIIPLDIYAPCLAGRTFYNRVQSVCFNEYDGFIYGGVSPDGILF
                     AYNPETNFLRMIGRVTPEKDIPCLTVGNDGIVYGISGEKDGMAHLFSYDPDSYSLKDL
                     GLLYTTIEIPWHGYEFSSACTGDFGEIYLGESDRISHLFIYFPPIPKRKKIT"
     gene            complement(16937..18151)
                     /locus_tag="PLF90_00920"
     CDS             complement(16937..18151)
                     /locus_tag="PLF90_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55879.1"
                     /translation="MKKEALEGEIEYLCFDKLPLHDSTWGLCTGVDGNIYIAACGECT
                     GGLSVFILRYLPDKEKLEYLIEVGPEVGEPADNGHATQSKIHYCLIPGSDGLLYCATH
                     ASGPPVNHPFWRPWNTWDDESVRFPGAFIFTYDPVKNKINNFGIGPKREGSRCLAYDE
                     KRKKLYGITWPRNHVYVYYIDSHTYIDLGRIGDINPQAIFLDKYGNAYTTDDYGFILR
                     IDAETNEIKKLKVRCPYENYRYGWHNVTYVVVPSPDWQWFYGTDWGYESRLWRFTPYK
                     EEGVMEDLGRAWGPVDFKTDRNLERWQVRGLVFGADKKLYFAMRSGWEDEEHTYIIRL
                     NTETGEREKVCSLTFGEHKPRAIASATFDFYGNLYFAEAGVSPTGMYIYRPNYVKKDK
                     KLFSWKDVKLWG"
     gene            complement(18132..19784)
                     /locus_tag="PLF90_00925"
     CDS             complement(18132..19784)
                     /locus_tag="PLF90_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOK55880.1"
                     /translation="MNSLFIGLIGLTLFAISYILYGKYLEKLWEIDERRKTPAYTKTD
                     GIDYIPAKHWLILFGHHFSSIAGAGPILGPVIAICVWGWGPALLWIILGSIFLGGVHD
                     FSSLVLSVRNEGQTVGEITKKLLGDKSRVIFSFFLWFSLILVVAVFSAVTAKTFISEP
                     PIVLPTFFLIIDAVIFGFLVYRKNFSIFYSTILCLFLLFLFFLTGSKVPVVIQFNPLK
                     IWIIILLIYSFVASVLPVNILLQPRDYLSSFVLFAGIIFGYIGLITTHPSVKAPFYIS
                     FFSQSGALWPMMFVIIACGAISGFHGLVSSGTSSKQIANEKDIRKIGYGGMLTEGFLS
                     ILALLCVSAGLFWKGPSPELNYPDLMKQGDWIGTFATGYGHILKRILDPKIGKLFAIV
                     MINSFVLTTLDTATRITRYITQELFGETFRIKPFKNRYLATVLVIVFSGYLAFGNWQK
                     IWPVFGASNQLVAGIILLLCGCYLLQKRKNALSTLIPALIMFMTTMVALTIQMLSFYS
                     QKHFLLGNISLILIILSIFVINESLKIILHLKRKEKYEKRST"
     gene            complement(19799..20719)
                     /locus_tag="PLF90_00930"
     CDS             complement(19799..20719)
                     /locus_tag="PLF90_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOK55881.1"
                     /translation="MPSIRELAKRESRFISGHRLCAGCGAGIVMRLVLLAIDKPVIAS
                     CATGCVEVASTIFPYSAWKIPWIHSAFENSAATISGIEAAYNVFKREGKIDKDIVFVG
                     FGGDGGTHDIGFQALSGAAERGHNVLYVCYNNQAYMNTGIQRSGATPKYAHTTTSPVG
                     KVIPGKIQWQKNLVEIMVAHGIPYAATSIPGRWNDLVNKVEKAVKINGFKFIEVLSPC
                     RLGWPHEPELTMEISRLAVETCIWPLYEVVEGKYKLTYKPKEKKPVSEWFKLQGRFRH
                     LLLPQNSGLLESIQKEVDRRWEDLLKKCES"
     gene            complement(20757..21947)
                     /gene="porA"
                     /locus_tag="PLF90_00935"
     CDS             complement(20757..21947)
                     /gene="porA"
                     /locus_tag="PLF90_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOK55882.1"
                     /translation="MGIKVAKTANEAFAEGMRQINPDVVAAYPITPATEIVQIFSQFV
                     ADGRVKTEFIQVESEHSAMSACIGASAAGARAMTGTSSQGLALMWEMLYIASGFRLPI
                     IMAVVNRALSSPINIHCDHSDTFGARDSGWIQIYSENVQETYDNLIQGVRIAEHPEVR
                     LPCMIMTDGFILSHCLETMEMLSDEEVQEFVGNPPERENLIKALIDGKPITIGALDLQ
                     DYYFEHKRQQAEGMRNALKVILEIGKEFGDRFGRYYEFVENYKLEDADIGIVVIGSTA
                     GTGKEVVDSLRKKGIKAGLLKIRVLRPLPVEDIKNSLKNLKVVGIMDRVEGMNAFCGP
                     LYSEISACLYDLEKKPILTDFVYGLGGRDVKIEDVEIIFNDLLKILEKGKKDFTLKYI
                     GVRE"
     gene            complement(21916..22209)
                     /locus_tag="PLF90_00940"
     CDS             complement(21916..22209)
                     /locus_tag="PLF90_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55883.1"
                     /translation="MKKKIYLLGVLLLSGWIFSGAEYLIFKNEKEKLNKQLIEKEMEI
                     TTYKSILGEVEKAQKDIDNIINNLNNLKLKLQTMEEKIKKGDENGNKSSENSQ"
     gene            complement(22206..22496)
                     /locus_tag="PLF90_00945"
     CDS             complement(22206..22496)
                     /locus_tag="PLF90_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK55884.1"
                     /translation="MEKKLTWKELPVGDILEGGSSLNFKTGNWRSKKPVHIPEKCINC
                     FICWISCPDDAIVVDSEKGKWSHFNYDYCKGCGICAYECPKKAIEMKDEGEK"
     gene            complement(22498..23076)
                     /locus_tag="PLF90_00950"
     CDS             complement(22498..23076)
                     /locus_tag="PLF90_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOK55885.1"
                     /translation="MKEIFEIRWHGRGGQGAKTAALLFGDACISTGKYIQAFPEYGPE
                     RMGAPVASFNRISSSPIRIHSGIENPDVVVVLDFSLVEQINVCEGLDEKEGILLINSP
                     LSPEEIKKKINFKGKVYTVDASKIAMETIGRNIPNTPMMGALVKVTGILTLEELLKDT
                     EKKLQVKFRNKPEVIEGNLKAIERAYNEVKGD"
     gene            complement(23073..23741)
                     /locus_tag="PLF90_00955"
     CDS             complement(23073..23741)
                     /locus_tag="PLF90_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55886.1"
                     /translation="MEIYKNLIKIKKQELFLFFITFLYVSLRSFLLEKFKTYYFFLFF
                     FEIYFTISVYAGIKKCVYDETFTFSDIFRDGFYFFPSILLYNLLISLLAGIIYLIALS
                     AISSIKILSFFSFFLFFIIVVWAALPFFYIFLTLYTPFIILAENETIFEGIKKSYDFM
                     KNKLSELINLFFPFLVFWVFFFTIFQKYDKITFLKIILLFLVSFLEILTIKTVFLVYK
                     GVKK"
     gene            23861..25042
                     /locus_tag="PLF90_00960"
     CDS             23861..25042
                     /locus_tag="PLF90_00960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOK55887.1"
                     /translation="MYRIGKEEVKAVEKVIKSKELFRVKSRKLSECDKFEKEWAKKIG
                     TKYALLLNGGGTAALICGLVGFDIGPGDEVLIPSYTFMATATAVLMVGAIPVVVEVDE
                     TCTMDPIDLEKKITKNTKTIIPVHMVGFPCNMEKIMNIAKRYNLKVLEDCCQAVGGSF
                     KGKRLGTWGDAGAYSFNYFKVISCGEGGGLVTDDRIIYEKASIFHDSGTAFRPYAGEF
                     SIPIFLGLQFRASEIMGAIMRVQLKRLDKILKDLRKNKRRIMEELIDYPGIKFIPSND
                     IEGDCGIMIGFRFDDENTAKKFVEIAGGYRPIDSGKHVWFDWKPVIEKRIWHKERLNP
                     YNFPENQNLRHTYTKEMYPKSIENLSRTVLISVNPDWKDKDIKEKVKKIKKAVEEVYK
                     K"
     gene            25039..25800
                     /locus_tag="PLF90_00965"
     CDS             25039..25800
                     /locus_tag="PLF90_00965"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK55888.1"
                     /translation="MNIEFFDVNVWFGNLKDDIKKISVSLKRISEKLDELNIKKAIVY
                     HISQRDVHPEFGNEILIKEIADKEKFYGIITLLPFQTDEIKKIDFKDLKNKKIVGFNF
                     FPKKHNYILDKITFGDFLSELEYKNFPVFFDLLSGFSVDYRDVYSILNNFPKLTCILC
                     NLGIWNTNRFTWPLIEKCPNVYLESSLLSLQEGGIEETVKKYGAERIVFGSGFPERYF
                     EASILQLVHAEINEKDKEKIAHKNIERIIKRIKYE"
     gene            25793..26590
                     /locus_tag="PLF90_00970"
     CDS             25793..26590
                     /locus_tag="PLF90_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOK55889.1"
                     /translation="MNKLWKEFLKKGKLEDCPVIDIHCHMGLFYGSHMPYTDPDTMAK
                     RMELAGVKLIVFCHNYALLYPEEGNKYNIEAVRKYPEKFKAYCGINPNYPEVVEKDLS
                     TFDKYRDVYVGLKLLPDYHKIPLSDKKYKPVFEFADKNKLIVLTHTWGRSIYDGADEV
                     EKILKKYKNLILILGHSIHGDWEKAIEFVKKYENVCLELCAILDERGIIEKFVYEIGS
                     DRILFGTDFPWFNHHYYIGALLGSGISEEDCRKILYLNANKILKIFE"
     gene            26587..27681
                     /locus_tag="PLF90_00975"
     CDS             26587..27681
                     /locus_tag="PLF90_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOK55890.1"
                     /translation="MINFLSAIIFLTIGVIVYNSNYKVIFKHDDLITVNEGKWESDFF
                     PVKEFNFYKLTFTSKSPYKNFWAIIFYDKNKNQLIADNYSSFDKSTDFTENTFYFRGK
                     CNAKYGKVIFQKIERNKIPFEIKNISIKNATEKEILYWMENLYKKIPEIKKFTIKKNY
                     IPETMKKLKKGENLRIVMLGDSIINDTGNSFFDILLKKIYPKAKIKIITSVRGGTGCW
                     YYQEENRVKEYVLDYNPDLLIIGGISNKNDTEAIRNVVKQVREKKPDTEIIIMSKAVG
                     KEGDPRTNPEWTFEIPDDESSYRYRLKKLSEEEKIEFFDIEAYWGEYIKNSNLPYDVF
                     LRDPVHANEIGRIVLAYLLYFYLIETDAEK"
     gene            27771..28391
                     /locus_tag="PLF90_00980"
     CDS             27771..28391
                     /locus_tag="PLF90_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK55891.1"
                     /translation="MVEYIKWLGHASILISIGEKNIYVDPWKLKKDQPKADLILITHS
                     HYDHFSPDDINKIIRNDTVIIGPHDILKIKTGEKIEIKPGQETDLKWVKIKGVPAYNI
                     NKNFHPKSNNWCGYIIEIGGNKIYIAGDTDFIPEMKNIVADVVILPVGGTYTMTAEEA
                     ANAVNIIRPKIAIPYHFGDIVGSEKDAKFFSSFILNSEVKILKIEK"
     gene            complement(28394..29452)
                     /locus_tag="PLF90_00985"
     CDS             complement(28394..29452)
                     /locus_tag="PLF90_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK55892.1"
                     /translation="MKNGKYLVGFIGFGGIARWAHYPGWKKLSDVEVIGISDPSENVR
                     NIAKNEMGIKYVFENYEELLKIEEIDIIDICAPNTVHFPATIASLKAGKHVICEKPLA
                     VSKKEVEGMIEQAKKSGKKLMAAQHMRFMKESVALKKLIDDGELGEIYFAIAYALRRR
                     HLPTSFTFIDKALSGGGPMFDIGVHILDLTYWFMGSPKVHSVKGITLTKLAKRKDIRG
                     LWGEWDREKYNVEDFASGFVRFSDNRALTLTCSFLANMEKIDDFSTTLFGTEGGIRWP
                     NGKLAKEKNGILQDIELRLDVLPNVAPHHEELRVFLECVKEDKEVPVKPEESLEVIKI
                     LEAMYISSDTGKEVIFDK"
     gene            complement(29470..31560)
                     /locus_tag="PLF90_00990"
     CDS             complement(29470..31560)
                     /locus_tag="PLF90_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOK55893.1"
                     /translation="MLKYFFEPESACLIGASTNPEKLGFKILKNLIDGGYQGKIFPVN
                     PKADTILNLKCYKNVSEIPDSVDLAIIVIPSEFVCDVAEQCGKKGVKGLIVISAGFKE
                     TGKEGKKREEKLKEIVKRYKMRLIGPNCLGIIDTKNKLNASFAFEMPPEGKIAFITQS
                     GALGTAVLDWAVKENIGLSKFISFGNKADVSESDLIEELENDPDTNVILLYLEGVSDG
                     RRFIDISKRVSSKKPIIVVKSGKTESGTKTATSHTGSLSGSDTSFNAATKQSGIIRAD
                     TVEELFDFAMIFSYQPVIKGNRIGIVTNAGGPSVMAVDMIEETGLKLAEFSNDTIEKL
                     KSFLPPASNIHNPVDVLGDAQADRYEKAIECVISDENVDSIMAILTPQVTTQIKETAE
                     CIVKISKKYDKPVIACFMGGKRVEEGIKILRENKIPNYFSPERGIVSFKAMFDYKKRI
                     EEKSKGSIVEFKVQKEKVKEKIDYFKKQHINVIGDVEGREILALYGINVINSFLAKNE
                     EECEKFLKEKEGVYVMKLVSPDIIHKTEAGGIKLNIRTPEEGKKAFLDIINSAKRYKP
                     DAKITGVQIQEMIEKGVEVIVGVNKDNQFGHLIMFGLGGIYVELLKDVSFRVIPVTDI
                     DVDNMINEIKTSKILKGFRNIPKVDINSVKETLLRVSQLVSDFPEIKEMDINPLIVKE
                     KGCIAVDVRFNFEI"
     gene            complement(31641..32927)
                     /locus_tag="PLF90_00995"
     CDS             complement(31641..32927)
                     /locus_tag="PLF90_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK55894.1"
                     /translation="MRGKIYYLLRIIKIGVKNIFLHKLRSLLTILGIVFGVASVIAML
                     AIGEGASFEAREKIKELGSQNIIVRSVKPPETRITQEATTFVSVYGLLENDYKMIKTI
                     PSVDLIVPTWEMKEEMRYMEKSIKGRIVCTYPEYKDVMNIGLEMGRFLTQIDIELIQP
                     YAVIGYSIRKSLFPTEDPIGKTITIRNYSFKVIGVAKEKGVTTGTTGFAAEDVNFDVY
                     IPFTTGRSYFGEFEIASKEGPVSKWERNWVKYHRFIVRVNNTDKILTTSAIIENILKI
                     NHKKDDYEILVPLQLLLQAEHTKRIFNIVLGSIAAISLIVGGIGIMNIMLATVTERTR
                     EIGIRRAVGAKRKDIISQFLLETVILSSVGGFIGILLGILIPKLVTKFAGMTTVITWW
                     SIALSFTISVGVGITFGIYPARKAAYLDPIQALRYE"
     gene            complement(32917..33606)
                     /locus_tag="PLF90_01000"
     CDS             complement(32917..33606)
                     /locus_tag="PLF90_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK55895.1"
                     /translation="MSNENNSIIIMRGVKKIYDLGEVKVPALKNVNLTVKEKEHISIM
                     GPSGSGKTTLLNIIGCLDKPTEGEYFLDSVNINQLDDDKLSEIRNKKIGFVFQNYNLI
                     PQLNVIENIGLPLIYRGIEEKEIVEKAKYYANLVGLGDRMYHRPTELSGGQQQRVAIA
                     RALVNEPVMLLGDEPTGNLDTKTGKEIMEIFIKLNEMGRTIVIITHDPKIAEYGQRVV
                     RILDGEIIDER"
     gene            complement(33603..35243)
                     /locus_tag="PLF90_01005"
     CDS             complement(33603..35243)
                     /locus_tag="PLF90_01005"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOK55896.1"
                     /translation="MKYLRPFKNRIFLGFILILILISVFYFFNSTDKQSKDIVTYKVK
                     KVDLPITIHESGNLVALKSTKITNQVPGRRTILEVVDEGTEITEEDVKKGKVLVKLDS
                     DDLETQRDQMEITVENSYASYLNAQENLEIVKKENDSMIKEAELKVTFAKMDLEKYLG
                     ASLANEVIEKKNKRENIDYKEIINSEKLGGEALNKKRQLENNIDLAKEEVIRAQNELE
                     WSKKLSEKGYITKNELEADQLSLKQKGVNLEQAQLSFQLFVNYEFPKNLEQLLSNYTE
                     AVAELDRTKSKAKSRLIQAESDLKSKRAAYKLNEKRLTDLETNIKNCTIVATAPGFVT
                     YSTSAGSFRALIQGVQIQPGATVWQSQELLVLPDFNSMGVDVKVHEASIQKISLDQIA
                     NVKISSIPNKVFKGKVVQISNMPDQSLKSLNPDVNVYVVKVELIDKDKAMRPGMNADV
                     EILLKELKNVIAIPIVAVNFEKGQAYCEVLEGKNIVRRNVKLGESSETMVEVKDGVKE
                     GDVVVLSYKGPTKGTVKKEEMAEKGKIETKTPEKEIKK"
     gene            complement(35221..36783)
                     /locus_tag="PLF90_01010"
     CDS             complement(35221..36783)
                     /locus_tag="PLF90_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOK55897.1"
                     /translation="MKRNILFIFSMVLLVSCSPKYYEKSADKEVYEILNKKSEKVKKD
                     KMPVNLSEDTQKETVILSLKDALILASNKNREYQTAKENVYLSVLDLTYQRYLFKPRI
                     TLTGNINWSKSDEETINSNLNLNLIQTLATAGEVTFNIGEAILQYLTGDKEKAFQSIL
                     SLNLFQPLFKGAGRKIALENLIQSERNTVYQIRDFLRYQKSFSIEITRDYLNLFLYKK
                     RMENYYTNYMNLKDTRERIEMLSEAGRIAAFQVDQARQNEYTAYQRWIDAQNLYYSSL
                     DSFKMKLGLSPSYNLILTDKEIEELFEKPLSEVKIDTEEFIKYALENRLDLITEYEKV
                     EDSKRKVEIALNNLKNSINMNASIKSSGDIKDRPNIDLNSPQYSLGIDFNIPFNKLPQ
                     RNEYKRALIQYERAKRSFDKKVDTVKKEIIDSYRNLEESYQSYIIQLNSFKLAERRVE
                     STDLLLQAGRATTRDLLEAQDAYLSAKNNVINSIVSFILEYLNFLYTTEKLEIDENGI
                     WKGDINEILKTF"
     gene            complement(36859..>37908)
                     /locus_tag="PLF90_01015"
     CDS             complement(36859..>37908)
                     /locus_tag="PLF90_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK55898.1"
                     /translation="IADYHISGYLKSGVDVVAISDIDTERAKQKAEKFNIKNVYSSYK
                     EMLEKEKEIDIVSICVPNKYHAEVSIECLKNGKNVFCEKPPALNAKETEKMVKTAKDA
                     KKVLMFDFNNRARPEAQALMKYIKNGDVGKINSAQALWIRRCGIPGFGGWFTQKSLSG
                     GGPVIDLLHMIDLALYFIGFPEPDFVLAKTFNDFSGNPDFKGPWGIPDVEGGIMDVET
                     SSHAFITFKTDQVLFARNSWAEMNKREEVSVTFQGTKAGGMIRRLFGRDGIDDTAIDD
                     CELYTMEYGKPVNRKIIVEPDPKMGRERAVINFVNTIRRQEEPFSKPEEAIILMKIID
                     AIYKSSEEKKPVKID"
BASE COUNT        12730 a         6206 c         4966 g        14006 t
ORIGIN      
        1 gttatccgcc aaaagacatg gcggacttcg gctttcgcct acggaatact ggacccgctt
       61 taaaggggat ttttaaaagc gggggatggg gcttggaaaa aattttattt tgtgagaatt
      121 atatacttgc cgagaccgaa ggatgtattt ttgcctatgt ggatgtaaga gccgatttca
      181 attaaaggat aaaatttacc tatatcacct ctatattcaa tttctcctaa tatccctcca
      241 agtttcattt cttctttctg ccttgtagaa tatcttttaa aatccttcca gtataaatta
      301 cattttccta ttttaacttt ttctgcctct tttattattt ctgaatagtc aatcttttct
      361 ttaaaatcac accagaaata aagaatctgc gaaatccttc ttaatactgc ctttataaat
      421 acctcaaatt ctggaactgt tataaagtca ccctgacttt ttattttcgt tggtgttaaa
      481 aattttatgc ttattttatc aatatctttt tcaatcaatt catattctct aacttctggc
      541 atctcaactg ttcctttcct aaaatcaaat atttcttttt caactggata tttctgaaag
      601 acctctttta taaaaaattt ctccctgaat tttcctattc ccttctcgcc aagatatgca
      661 aaagatataa ggaagtatgg aaaatattta actgcttttt caaaaaggat aaggttaaat
      721 tcaaaaatct catcttctct gtaaacattt tttttgttca ctggaaattc aataacataa
      781 ggtcttggta tttcttctat cttttctttt atttttttgg gttcaaagag attgacataa
      841 acacaatctg aaaaggaact gcaattttta caatctgttt catttttaac acatacattt
      901 tttttgaatg aataaccaag tccacctcta attgttgcac ctttaaagaa aggaagtgtg
      961 agagttttat ccggttgaat tctgaaagag ataattataa acttcaaatt tttaataaat
     1021 tcaaaatcca tttttaaatt ttttcaccaa aagtaatctt ttctgtatta tagcatattt
     1081 atcatattac cagaattcat taactcttgt ttgacaatgc agattttgaa aattagacac
     1141 tgatttgaca gaattagaaa aacatgagaa tataaaaaga tgaaagtcat taaattgaaa
     1201 aaaaggggaa attatgaaaa aaattgtttt cttttatttt ctggtcataa ctttaatctt
     1261 ttctcaggaa attcctcaaa aaataaagga tatacttggt aaaactgaaa gtattttaaa
     1321 ggacaaaatg attgaaagca gaattgagag ttcttataag atgatgggta tgactacatt
     1381 gtttgatata agaattataa ctgatttgaa aaccgagaaa ttttatatgg agatgggaga
     1441 aatgggtatt actgtgtatg atggagaaaa tctgtggact tacaataaaa tgtcaaatct
     1501 ctatacaaaa attccaattt caaaagatga attagaaaaa aataaagaaa gttttatccc
     1561 tacaagtata aaagaaatat ctgaattttt accgaaactt gatagttcct ctattgaaga
     1621 agtcaatttt gatgataaga aatgttacag aatagaatta actggtaaag ataaaaggaa
     1681 taataatgtt aagttgacat tatttattga ttcaagtaat tatctaccgg ctcaatttga
     1741 gatagaaagc aaatttccat ccattaatga accaataatt attaaaaaca ggattaaaac
     1801 tcttaaaatt aatccagaag ttccttcaga tatatttgtt tttaaaccac caagtggagc
     1861 gaaagaattt aaattttcgg ataccagaac ttctaaagaa gaatctcttg aaggaaaaaa
     1921 agcacctgat tttaatctga aggatattga agggaaaagt atcactcttt cttcttatga
     1981 aggtaaagtt gtacttctta atttctgggc aagttggtgt ccaccatgca gagaagaatt
     2041 aaaagtcata cagaaaattt acaatgaata taaagataaa ggagtaatag ttctgtgtgt
     2101 aaatagtggt gaagataaag aaaaagttga ggattttata gatgaaaaca attataaatt
     2161 tcctgttctc cttgatacag atagcaaagt aagcgataat tataaagtaa acaccattcc
     2221 aagagttctt ctgattaata aagatgggat agttgtaaaa gatttaacag gttatacttc
     2281 tgaaaatgaa aaaattttaa gggaagaaat tgaaaagata aaataggtta actatatctg
     2341 attttgtaag aagagaagaa aatgaactta tattcagtta aaataaatcc tgtaaagaaa
     2401 attttaggaa ttcatatatt attagcagtt ttggtttttg ctcaaattaa ctttccgcca
     2461 aaaattaaag aagtactgaa taaaactgaa aatatattta aaggtaaaaa aattgaagca
     2521 aagtatattt tttctacaac aataggaaag tatcttgatt ctgattctaa acagaaaaat
     2581 tatgaaataa ttgaaataga atcaaatctg atggctgatt taaattctgg aaagttttat
     2641 cttgaaaatg gagatagaca gatttctgtt tatgatggaa attacttatg gaattacgat
     2701 aaagtaataa atgcttacaa taaaatacct ttaaaaaata ctccttccca ttttgttttt
     2761 cccttattta ttatagattt agcaaaagca attcccaaaa ttgaaagtgt tgatatggaa
     2821 gaggtaagtt ttgaaaataa aaattgtttt ttaataaaat tttctggaaa ggactttgca
     2881 aataattcct gtactgtaaa tttatggatt aataaagaaa actattatcc tgtacaattt
     2941 caatcattaa caactatagg aactgaatta tccgaaatga attataagtt taaaagtttg
     3001 aaaataaatc ctgaaatttc tgatgataag tttaatttca aaattccaga aggtgcaaag
     3061 gagataaaaa ccggtaagta aaaaaggagg agcatatgaa aaaaataatt ttatttttga
     3121 tttttgtaaa ttttttattt gctcaaagta aaaaggatgt taaacttgtt atttacaatc
     3181 agaattttgc tgtatgtgat gagataaggg aaattgaagt acctgctgga aaaaatgaac
     3241 ttatttttac agatatacca agatttattg aaccggtaag tattcagatt tcagttttat
     3301 caccttctat aaaatttaat gttcttgagc agtcatttaa ttatgatgtt tatacttctg
     3361 ataaagtttt tgcaaaaaat ataggagaaa aagtaagtat gataacaaaa gatggacaat
     3421 tctattcagg aattttactt ttttatgatg ctggaaatat tggaatagaa acaaaggata
     3481 aaataatttt tgtcaataaa gaaaatttaa gagaggtaag ttttggaaag atgagcactt
     3541 ttacatataa accacagtta aaattccttg tacagaatga aaaaaaggat atatataaaa
     3601 ttcttcttaa atatactacc tcaaatatta attggcgtgc agattattta ggcagttttg
     3661 atgaagaaaa atcagtttta agtttaacag gatttataac agttaataat aaatgtggta
     3721 tagattatga aaatgcatca attgaacttg ttgcaggtga agttaaaaaa attgctgaaa
     3781 ttgaaaacca aagaaaagca ttcggagcgg ctgatgcttt aaaagcagaa atggcaggtg
     3841 ctccttcatt tgaagaaagt ccagtttttg agtatcacag atatacattg aagggaaaaa
     3901 cagatattaa ggatggagag ataaagcaga taaattttgt aaataaaaat gatttaaagg
     3961 ttgaaaaaaa atataagtat gatggagcaa ttacaaggta ttatcattat gataactgga
     4021 gaaatctgaa ttataatgat aaggttgaaa tctggattga atttaaaaat gagggtgaaa
     4081 ttcttcctgc aggaaagatg aagattttta agaatgaaaa ggaaggaagt atttttatcg
     4141 gagaaaacag aattcctcat acaccggttg gagaaaaggt tagattatca cttggtaatg
     4201 tttttgatat aaaaggagag agaaaaatta tagcacacga aaggataagt cagcaggttt
     4261 ataaagacac atatgaaatt aagattaaaa acttcaaaaa agaggatgtt tctgttgaag
     4321 ttattgaaca cttatatgga agttgggaaa taacagaaaa aagtcataat tatgaaaaag
     4381 aggatgcata tacaataaaa tttcctgtga aggttaaagg cagtggagag gaaataataa
     4441 gatacacagt tataacaaga ttttaaagga gaaaaaaatg aaaaaagttt taattattct
     4501 gtttataatc ttttccattt catatgcaag gacaaaactt gttgctcttc cggatagaga
     4561 aatttcttac atcagatttg atgctcctta tactgctttt gttgaagaag aaagaatact
     4621 gacacttcag aaaggcataa atcaggttga tttttcatgg aaaggtgtga atattgacca
     4681 ggactcaata aatctaactt ttctcacaaa tcctgataaa cttaaattga taagtttaag
     4741 ttatccacct gaaggaaatt ctcttatctg ggaaatatac agtcccgaag gcggtgagga
     4801 aaaagtaaga atatcatact tgctttcaaa tattgactgt ctttacactt acaaaatagt
     4861 tgttgataaa aatgaggaaa atataaactt tacatcatac attattttaa gaaatttttc
     4921 tggagaggac tttgagaaag gaattgttac acctgaattt gggaagacat ttgaaacatc
     4981 aactaaaaat gaagaaacaa aacagatatt gatttatcaa aaggatggaa ttaaatttga
     5041 gaagattttt gtttttgatg cttcaagaat gatatgggac cctgaaacag caaaagaaaa
     5101 tgttggaatt cctgtatatt ttgaaataaa gaatgtaaaa gagagtggac ttggagaatt
     5161 tcctttactt ttaggaaaaa caagaatgtt tcaaattgaa agttccggta gttcaatttt
     5221 tcttggagag gacaatttaa aatacatacc tgttggagaa agttttcgtc tttattttgg
     5281 agatagcaga gatatagttg taaagcagaa aaaaatgaaa gatgcaaaaa taaatataag
     5341 aaaaaataat tctggagata ttgtacttta tgatactgat gaaattataa gaattgaaat
     5401 tgaaaacttc agagacaaga ttgcaaaaat tgaaattctt gagaatatat ctgatgaatg
     5461 ggatatggaa gaaagtactc acaaatatga aaaaattgat tcaaataata taaaatttgt
     5521 aattgattta aaaccgaaga gtaaagagat tgttgagttc cattatcaca ggaggaattt
     5581 aagatgaaaa ataaaatatt tttaattttt ttgatttttg ttttaaatct ttttgcaaag
     5641 gttgaccttg ttactttacc ttcaagggaa aaagttcaac tcacaattta taattctgct
     5701 gatttaacac ttgtaaggga tgagagaata ctgacactaa aaaagggaga aaacagttta
     5761 cagttttcgt gggcaaatac acttattgac cctacatctc ttgaccttat accaaaacag
     5821 gatgttgctt ctataaatat ttctcaactc atttttccac caagagttag cgaacttgga
     5881 ttatggaaaa ttgagtcaaa gttttcaaaa aagattccat ttgaaatttc atattttaca
     5941 tctggaatat catgggaagc attttatata gggactttat caaatgatga aaaaagtatg
     6001 gaattgaaag gatatgtgaa ggttataaat aaaagtggag aggattatga aaatgcatct
     6061 gttcgtcttg ttgttggaaa aataaattta cttgagatga taagagttct tgcaaaaaga
     6121 tatcctccct atggaactcc tggatattta ccacctgctc cattgataga aaaagaaaga
     6181 ggagttgaaa tgttaaaagc agcaaaggca gttttagcag aagcaaagga aaaagagatt
     6241 ataaaagaag gggtttctga atacttttta tatacaattg agggaacaga gaacttacct
     6301 gataaatggt caaaaagatt actttcattt tcacaaaagg atattcctgt tgaatttttt
     6361 tataaatatg aagaagaaag atatggaaaa aatgcaagaa gatttataaa atttgcaaat
     6421 gaccagaatc acaaacttgg aaaagaacca cttcctgatg gattgataaa aattttcaaa
     6481 tatacaaata aagaaggaaa tttatcctat atgggttcag ataaaacaaa atatataccg
     6541 attggagaaa aagttgaact tgacctcgga gaaagtaaag aagtgaaaat tgatactaaa
     6601 ttgatggatt tgaaaaaaaa gaattttgtt tttaataagg atggtaatat ttctggctgg
     6661 gaagaagaaa aaatatataa aattaaggtt acaaattata aaaatttacc ggttaaggtt
     6721 gaaataaaga gaaatttccc ccatcagtac tggaagattg aggttgataa aaatattcag
     6781 tgggaaaaat atgatatagg cagtgtaaaa ttcacattaa ctcttgcacc ttttgaaaca
     6841 agagaattca catataaact tattttattt gaaggtgacc gcagatatta atcctgccca
     6901 cctttaaggt ggacattgaa gatggttgat tttattagtg ttttcaatat caatttttaa
     6961 tctcaggaat taagtaaaga gtgtcactgg ttaaaacaaa taaacctttt tctgtaagta
     7021 taatatcatt tatatatttt gttttatctg ttttgttctg ccatattacc tcaacatttc
     7081 cgctttcttg ttcaaaaata atcatatttt gtttgctgac tactaaatat ttattttcta
     7141 atggaataat tgtaaagggc tgtttttttt cattccataa agattcttct tctgatggac
     7201 tttttattat atatcttttc agatttctat ttttaatgtc aaaatcaaat atgtcgcata
     7261 tccagtattt ttctgaaagg gaattatctt tttgtacaat cattttggga aatactgata
     7321 atattaaatg atttttattt tttttaaaat catcaaaatc taatagtcca gcaaagttta
     7381 accaatcttc tgtcttttgg taagaatatt taaatttttc atactttcct gtttcaggat
     7441 tatatttgaa aattgcggtt tcgtcttctg ccgaacaaat taatggatcc ctttttccta
     7501 caacaatcca aaaaattttt tcctctttat ctgatttaat tccagcgatt gtctttcctt
     7561 ctattccttt tttattttcc tcctgctttt ttgaaaaaat tatttttgat attcctgttt
     7621 ttaaatctat ttcaataaga ccttttacag aaccctctgc tattgcataa agtttatcat
     7681 taagaatttc aagagttttt atattttcac agacaagacc attttcttca ttcattatgt
     7741 aaaggggtct ctctgatgga aaaacataaa tacccgaatt tgaagcaata aaaatatcat
     7801 ctttataatt tacaattgaa tttaaagaaa agttttgaga taatatttta tgatattgaa
     7861 aggaaagcgg tttaaagttt tctttattta attttaaaaa gcccatatat ccatcagaac
     7921 ttaaaactac aataccacaa tattatcttc ccagattatt tttctgaata tggttgattt
     7981 tttttctttc ataatgaaat ttgggatatt agaagaaaaa aggtcatcaa ttgacaaaac
     8041 aggaatagga ttaatatttt tcacctgtat agaaatactc tgtgctttgt aagtttttct
     8101 ataatcattt ataaaagcga tgaattgagg aagtattgat tgtcctgagg tttaaaaata
     8161 tttttttcaa gtacttcttt tattatttct ttattatctt tattgttttc tatgagtcca
     8221 ctacttgtaa gataggaagt aatatttgtt attaaaagtt gttgccaatt atcgtatgaa
     8281 taatttttat acttattatt tttatgagcc catacaatta attcaatata actttctata
     8341 atcatctcta aaacttcttt ataaatttca ttcttctgtt cactaaaaag tatctaacaa
     8401 tttcacaacc tgtatgaaaa agaaattcaa caacattaaa atttggaagt ttttctaaat
     8461 tttcatcctt aagaatttca aaatattttc tgtaatgagt ttttaatttt tcaagatttt
     8521 tttcaatttc ccatggaaaa aatatctgta ctctttcttt actaagtttt atcagtatat
     8581 tatcaccttc atgtaattca ctcagatatt tttctatttc tttttctact tgagagatat
     8641 ttttttcatc aagccattcc acatctttat attttttaaa aatcacaggt cttacaattc
     8701 cttccagtcc tataacccag aattcggttg ttatatctgg aataattatt tttttctcaa
     8761 tacacaatga taatatctcc cttgattttt ctattgctac tttaatatca ggacaaaatc
     8821 ttattccata attagcaact ctaagaatag tgtataattc cattgttctc attttttcaa
     8881 aatttttatt ttttgcaaga taaaaagggt cattcaatac aatatcttta taaaaatcaa
     8941 ttaactccca aaaattttca tggacttttt taattatttg ctgtgcttca ctgtcatatt
     9001 tcaattcttt tgtcgttgga agtgaatcag taatggaaag taaaaaactt atcaaattat
     9061 ttttccatat ataaaacacc tttttatcct tatttacctg ttcaaaaaat ttatcaatca
     9121 atttaataca tttaagaaac atttttaatt tttcctgtgc atcttttttc tctcctaact
     9181 tataagttaa aagtagcagt aattttgaat tatagtcagg tgagtcagga gaaatagcaa
     9241 cggcactttc aatcaatgat aatgcttttt ctgtttctct caattccata tatcagacag
     9301 tttatttatc tctccttcct cctttatttc aaatatcttt ttttcattct gccttcccct
     9361 tatataaact tctacaatat attctttttt ttctctattt atcctgtatg aaccatctat
     9421 atatattgca gaagaaatca atttttcagg caatcccttt actatatctc tttcttccat
     9481 caatatacct gttcttttcc tctccagcaa tataacttca ggatacaaac ttaaaatctc
     9541 ttctatattt atcttaagtt catctcctat ataatcccac ttccttgtta tctcctcact
     9601 ccttatctct gtaagtccta aaagtatcaa ttcctctatc ggtattttta tcctctttat
     9661 tctctgactg actacttttg atatatcctc tgctattttt ccgtattctc ccttactatc
     9721 tggtagtaac attgaactgc tccagagttt tataccataa tgcgtttcaa caagttgaaa
     9781 tcttatatag tttttcttct ctttttgtcg tagttctatc attaaaaaac tgtcagcaaa
     9841 aagaagtttc cctacctcta ttacattttc tcctgaaaaa cttattgaaa tttgctgttc
     9901 cttaaaaatt ttttctatct cctctctttc aattaaagca atttcaggat agttgttaaa
     9961 tgaaaaataa atgaaatcaa aaagttcttt cggtacttta tctttaccaa tgtctattat
    10021 cgcaagtttt atattatctg agtaactgaa aaagaaaaaa gagaaaataa aaaataatat
    10081 taaaaataaa attttactct taaacatttt tatttactga ctattttggg aatcaatctt
    10141 cctgcaatta aaactgctgc attctgaaga gaatttttac ctgcaatatt ttctgaaagg
    10201 tcaataccta tttctgtctt tcaactgtaa gaatttgtcc gttatcaacc tttattgctt
    10261 ttatttccac ccttccctta catattgcaa gcccaccttt tcttgtccca aattcgctga
    10321 atgcttctcc tgttattaaa acttcaggtt ttttctctga tttatttcta tcaacaattt
    10381 caaaaccaag ttcttttaaa atatagacaa gttctgtctc aactgctggg tcaactacca
    10441 actgccttaa atgactttct ctgacttcta ctgcaactac agttaatttc ttgccttcta
    10501 tcagtttttt aatactattt attacatcct ctttcttttt ttcttttacc ataagagtat
    10561 ccgttttttg caatattaca ttccttatct ttgccgaaag ttttacaact tcatcattca
    10621 aactgtcttc taatggaaat acaatcattt ctgtgtaaac ccttcctgtc tctactccaa
    10681 taatttttgc tgctatgtaa agtttatttt tatcttcaaa aactctacca gtaattaaaa
    10741 cttttgcgcc tgttaaattt ccaattttta ccgctgaatc cgaagaaatt gttcctgtca
    10801 ttccaagttg catctcttca agtattctgt ttaaatcctg cctttcaaca agtattattc
    10861 tttcatcaag tgaaagattt gcagatataa tagaggaaac ttgggatccg attctctcca
    10921 tttttggtat agttgattca aaatcagaaa ctgcaacact tataaattga ttgcttttta
    10981 cttcagaata agaaaaacca actataaaaa gaaatgagat aataaaataa ctaattaaga
    11041 ccttcttcat aaaacacctc ctttttaatg tttaaatttt caagtgcaat attgaaaatt
    11101 ttaataaaaa gttcttgata ttcaggactt gtaatgttat ctataattgg catttttaca
    11161 tctattctta aaccatggtt ttcttcaatc ttgatataag gaatttctat attttctgtg
    11221 tgaaaaacaa taattaatgt tcctgaaggc atttttttcg gaatattatc tctttcatct
    11281 ataatctcag taaaaataat agatggtttt tcaactttat ctaaatcgtc aaaataaatt
    11341 ttttcaaatt taatctcctc ctttttaaaa aattcactca gtttttcaat attccctgga
    11401 agataaactg aatacttttc tgagaaaatt ttaaatggtt ttaataaatc aggaggatat
    11461 acatataatt ttgtggcacc tataattttc caactttttt cttcaggaat gtaaatctta
    11521 tatcttatta aaaattcact tttattcttt actgatggaa cttcaaaagg aaaatttatt
    11581 tcagtatatg gtttttcttt tattttttct tctattgtaa tagagatgtc ctttttaaag
    11641 gcaaagcagt atttcttgca atctgaaaaa tatctgcatt tatatttact ttttcaggta
    11701 tttttccgta aactataaga ttgagaatta ctttctgtcc tccaaaaaca caatacactt
    11761 ttttcatttt ctttttcgta atttatccct tttgtttgaa taatctgaga aaaacagaga
    11821 aagtgaaaga aaatatatat gaaaaaaata aatttaaaaa tttttttaaa ttcccctttc
    11881 atttttaccc tttttataaa aataataaaa tacacttttt ttgtcaactt ttttattcaa
    11941 agttaaatga cttataaatc tattgtcctt ccttcatctc caaatcccac ctttaaggtg
    12001 ggatttagtg ggacttttct ttttgtttta atttttccca gttttcaatt acttcttttt
    12061 catcctttac tttcttattt ccaaaaacat gaggatgtct tttaatcatt tttttgcaga
    12121 gatagtcaat aacttcatca attgtaaata tatccttttc cttcataatc tgagaatgaa
    12181 acacaacctg ccagagtata tcccctaatt cttccttcat cccatcataa ttttttttat
    12241 taacctcatt ggtaaattct tctgtttctt cttttaagta tttcaaaata ctttcatttg
    12301 tttgtttttt atcccaggga cagttttctc ttattttttc cataattttc agaaggtctt
    12361 taaaattata ttttttcttt tttctcattt tattcagccc atatattctg aaaacttaac
    12421 cactgcattg gatattttaa tataactttt tctatttttt ttacactttc attcagaaga
    12481 taatctattt tttcctcttc acttaagttt tcaggtggat agttaatttc ttcaaaaaaa
    12541 agtttatatc tatcattttc cctaacaaaa aatcctgaaa taaattctgt cttcaatttt
    12601 actgcaagag tagcaggtcc tctcggaaaa attgctttct tccccataaa attaacaact
    12661 tctccttttt cagtaaaaac cctatctccg agtatcgcaa ggactttatt ttccctcttg
    12721 aacctgataa aatctttggg atttgagcca gtaaaaatta cttcaacccc tttactttcc
    12781 cttattcttc tataaatttc tgttatcttt ggtgtcatat acggaattgc aattgcacat
    12841 attttataac caagtattga ggtaacaact cctgccagtt cccagtttcc aatatgtgct
    12901 gttaatgcaa taactcccct ccctttcttc aacgcagaat caagaacttc aatattttca
    12961 acaattactt tctcttttac cttttcataa ttaagtttcg gaattgtaaa aaattcggtc
    13021 atatatctgc caaaatttat gtaaactccc ttaacatatt tattcaattc tttttcgctt
    13081 aaatttcttt tttctttaaa ttcaagaatt attttgagat tttttttcat tatctttctc
    13141 aatgaggggg taaaaaaata tcttaattcg gtgagaatat aagcaataaa ataacagatt
    13201 ttttcgggga aatattttcc tataaaagag aaaattatgt atgttatgtc aaaaaaaaac
    13261 cttataattc tctcctccct tcaatcgctt ttttcaatgt ctggctatct atatattcaa
    13321 gttcacttcc aagaggaagt ccaatcgcta atcttgagtg tttaattcca agtttcttta
    13381 atatttctga tatatattcg gctgtattat ctccttctac ggttggattt gttgctatta
    13441 taatttcttg tatattttca ctttttattc tttctataag ttggggaatt ttaagttgtt
    13501 ctggagtaat attatcaagc attgaaattc tgccccataa aacatgatac cttcctttaa
    13561 aatttgtttt ttcaatagaa attaaatcct taacctcttc tacaatacat aaaatatttt
    13621 ctcttttttc atcaaggcat atattgcata actctttttc agcaaaatta aaacatctct
    13681 tacacaattt aatctgtttt tttaaagata taatcgtttc tccaagagaa atcacttcat
    13741 tatcatcgct ctggattaaa taataaacaa ttctttcagc acttttagga ccaatgcccg
    13801 gtaatttttt cagtttttct ataagtgtct ctattatttc aggatataat cccattttta
    13861 aaatggaaaa ccaatctgtg atttaagttc actggcaatt attttttcta ctttacctct
    13921 tgcttcatta aatgtttttt taataagttt ttcaagattt actttatttt ctggcttcaa
    13981 taatgtttca gaaatttcaa tgctcaaaat atccatcttc ccatttattt taatctttac
    14041 ttcattacca gaagaggaaa cttcaactat ctttgattca agcatttttt gaaactgaga
    14101 tgcctgctgt tttaattgcg ccatctgctt cagattatca aatattccca tttcattcct
    14161 ccatttttat tatttcgcca ttaaaaaact ctattatttt tttaacctcc ggattttcaa
    14221 tgattgactt actgttttta tttaaaatcg gtattatttt atattttttt ccggttaaat
    14281 caaaaattat tttttctatt tttatcttgt tagatatgtt ttcaaccatt gatttatgga
    14341 aattatactt actatcaaat aatatgtaaa tattttctcc ttcaattcta tcaagttttc
    14401 cttctctcag tgctgcttca agtgtgggtc tgaaatgttt aacttttttc aaaatttctt
    14461 cccagttgaa ctctttattt tctttttttt cttcaatttt ctgtccttct tctttctcat
    14521 ttttttcagt ttctttttcc tccttaaaat caaaaattgt tcccatattt tctttttttt
    14581 catctgtaat ttcttcttct ttcttaatct tattttcctc ttttttctca atcttaattt
    14641 cttcttttaa ttctttttta ttgtttcccc acaactgaag taatttcaaa ataagaactt
    14701 ctgtaaataa aacaggttct ctttctcttc tcaatatact cttaaattca acaatatatt
    14761 caataccttc aaaaatttct tctgttttta aattattaaa cctttttacc agttgctcat
    14821 ctatttcaat tttttcaata tcaagtttag aaatcactat atcctttact ttttttataa
    14881 ttccttcaag aatcaatacc gggtcttttc catcagaaat aagtttatga agattttcta
    14941 tactttttgt ataattttta tccagaatat ttttaagtat ttcctctata ctcttttctt
    15001 ctggaagccc gagtaaatac ctgacatcct gatgttttat tttctcatca gaaaaaacag
    15061 aaagttgttc aagtaaactt atggcatctc ttaaagaccc ttcacagaaa tcataaatct
    15121 catctattgc tttttcctct atttcaatat tctcttttcc acatatttcc atgattttat
    15181 ttttaacttc atctttttta agcggtttca aattaaatct ctgacatctt gaaattatag
    15241 taagtggaat tttttcagga gcagtagtag caaagaaaaa ttttatgtaa gggggtggtt
    15301 cttcaagtgt tttgagtaaa gcattgaatg cttccggtgt taacatatga acttcatcta
    15361 ttatataaat tttaaatctt gattttgcag gtaataaatt cacattttcc cttatatttc
    15421 ttatttcatc tattcctctg tttgaagcac catcaatttc aagaacatca agagaagcac
    15481 tatttgttat ttctatacaa ctgtcacatt tattacaggg atttagagca ggaccttcaa
    15541 cacagtttaa acatttagca aatattcttg ctattgttgt tttaccaact cctcttggtc
    15601 ctgcaaaaag ataagcatgc gcaactttat taaattttat tgaccttttt ataacttcaa
    15661 caatatcttt ctgcccgcaa acttcatcaa aattctgagg tctgtatttt cttgcaagta
    15721 ctttatacat tttaaatata attttaccac tttcaacttt aatagacaat tttcaggtta
    15781 tttttttcct ttttggaatt ggcgggaaat atataaataa atgacttatt ctatcactct
    15841 ctccaagata tatctcacca aaatctccag tacaggcaga agaaaattca taaccatgcc
    15901 atggaatttc aatagtggta tatagtaatc caaggtcttt caaagagtaa ctatcagggt
    15961 cataggaaaa taaatgtgcc attccatcct tttctccact aattccataa acaattccat
    16021 cattacctac tgttaaacaa ggtatatctt tttcaggagt gacccttcct atcattctta
    16081 aaaaatttgt ttcaggattg taagcaaaaa gtataccatc tggagaaaca ccaccgtata
    16141 taaatccatc atattcatta aaacacacag attgaactct gttataaaac gttcttcctg
    16201 caagacatgg agcatatata tcaagaggta taattttgtc ttcttccgga gaatacttaa
    16261 acatctgaca taactgaccg aaaaaataga cattaccttt attatctttt acaagaacag
    16321 aagaaaaaag tttattttta tctatctgtc ctttgattga aacttcattt tttgttatat
    16381 cataaatgaa aaagtatcca ctctcactgc ttattccata taaaacattt ttatcctcat
    16441 cacatattaa agaaacaata aattctcccg gtactggttc tgaaattatt tttatttctc
    16501 ctgaaaaagt ccctccttct cccatatggt caccagatgt atcataacag aaaatataac
    16561 ctttctctcc ttcgctcact ccaccccata tctttcctgt tttatcaaca cataaagaat
    16621 tttttacact ttttgctccc tcaattatgc ttatatcaca gacaccgtct ccctcgggtg
    16681 agaaactgta ataaaaaaga tgacttctct ttccacttgt tgcaccataa atttttctgt
    16741 ttttcccttt acagagagat gttattgcac actcttcagg aggaattggt tctgaaagtg
    16801 attggaaaag ccgtaaactc ctattttcaa ctcttccata tgttacatag gaattatatt
    16861 ttttaaaatt tatatgtatt tcaggtcttt ttggccactt cctcttttca agttcttttt
    16921 ctaaattttc cattttctac ccccataatt ttacatcttt ccaggaaaaa agttttttat
    16981 cttttttaac ataattcggt ctataaatat acatacctgt cggtgaaact cccgcttctg
    17041 caaaatataa atttccataa aaatcaaatg ttgcacttgc aattgccctt ggtttatgtt
    17101 ctccaaaagt taaagaacag accttttctc tttctcctgt ttctgtattc aatcttataa
    17161 tataggtgtg ttcttcatcc tcccatccac ttctcattgc aaaataaagt tttttatctg
    17221 ctccaaaaac aagccctcta acctgccatc tttcaaggtt tctgtctgtt ttaaaatcca
    17281 caggtcccca tgctctccct aaatcttcca taactccttc ttctttgtaa ggtgtaaatc
    17341 tccataatct tgattcatat ccccagtcag tcccgtaaaa ccactgccag tcaggagaag
    17401 gaacaacaac atatgttaca ttatgccatc catatctata attttcatag ggacatctca
    17461 ccttaagttt ttttatctca tttgtttctg catcaattct taaaataaaa ccataatcat
    17521 ctgttgtata agcatttcca tatttgtcaa gaaaaattgc ttgaggattt atatctccta
    17581 ttcttccaag gtcaatataa gtatgggaat caatatagta aacataaaca tgatttctgg
    17641 gccatgtaat tccatataat ttttttctct tttcatcata tgcaagacat ctactaccct
    17701 ctcttttagg accaatacca aaattattta ttttattttt tacagggtca taagtaaaaa
    17761 taaaggcacc aggaaatctt acactttcat catcccatgt gttccatggt ctccagaaag
    17821 gatgatttac tggtggacca cttgcatgtg ttgcacagta taataatcca tcactccccg
    17881 gaattaaaca ataatgaatt tttgattgag ttgcatgtcc attatctgct ggttccccga
    17941 cttcaggacc aacttctatc aagtattcaa gtttttcttt atcgggtaaa tatcttaaaa
    18001 tgaaaacact caatcctcct gtacactctc cacatgcagc aatgtaaata ttcccatcaa
    18061 cacctgtaca taatccccag gttgaatcat gtaaaggaag tttatcaaaa catagatatt
    18121 ctatttctcc ttcaagtgct tcttttttca tatttctcct ttcttttcag atgaagtatt
    18181 atcttcaaac tctcatttat tacgaatatt gagagaatta ttaaaattaa acttatattt
    18241 ccaagtaaaa aatgtttttg agagtaaaag gataacatct gaattgtaag agcaaccata
    18301 gttgtcataa acattattaa agcagggatt aatgttgata atgcattttt ccttttctgc
    18361 agaagatagc aaccgcataa aagtaatata ataccggcaa caagttgatt tgaagcaccg
    18421 aaaacaggcc agattttctg ccagttacca aaagcaagat aaccagaaaa tactataaca
    18481 agaactgttg caagatatct atttttaaaa ggtttaattc tgaaagtttc tccaaataat
    18541 tcctgtgtta tatatcttgt tattcttgtt gcagtatcaa gagttgtcaa aacaaaactg
    18601 tttatcatca caattgcaaa taatttaccg attttagggt caagtattct ttttaaaata
    18661 tgtccataac cggttgcaaa tgttccaatc cagtccccct gtttcatcaa atctggataa
    18721 tttaactcag gactgggacc tttccagaaa agcccagcag atacacataa aagagccagt
    18781 atagataaga aaccctcagt tagcattcca ccataaccga tttttcttat atctttttca
    18841 ttcgcaatct gttttgaact cgttccactt gaaacaagac catgaaatcc acttatagca
    18901 ccacaggcaa taataacaaa catcattggc cagagagcac ctgattgaga aaaaaatgaa
    18961 atataaaaag gtgcttttac agatggatgt gttgttataa gtcctatata accaaaaatt
    19021 ataccggcaa aaagaacgaa agaagaaagg taatcccttg gctgtaaaag aatatttaca
    19081 ggtaaaacgc ttgctacgaa actgtatatt aaaagaataa ttatccagat ttttaatgga
    19141 ttaaattgta taacaacagg tactttactt ccggtcagaa aaaataaaaa aagtagaaaa
    19201 agacatagaa ttgtggaata aaaaattgaa aagttttttc tatatacaag aaatccaaaa
    19261 attactgcat ctattataag aaaaaaagta ggaagtacaa ttggtggttc tgaaataaat
    19321 gttttagcag ttactgcgga aaaaacagca acaacaagga ttagagaaaa ccataaaaag
    19381 aacgaaaaga ttaccctgct tttatctccg agaagttttt ttgttatttc tccaactgtc
    19441 tgtccttcat ttctaacact taaaacaagt gaggaaaaat catgaactcc gccaagaaaa
    19501 atagaaccta aaattatcca gagtaaagca ggtccccatc cccaaacaca tatagcaata
    19561 acaggaccta gaattggacc agcaccggca attgaagaga aatggtgtcc aaagagaatt
    19621 aaccagtgct ttgcaggtat ataatcaatc ccatctgttt ttgtataggc aggtgttttt
    19681 cttctttcat caatttccca taatttttcc agatatttac cgtagagaat ataggaaatt
    19741 gcaaataatg ttaacccgat taaacctata aataaagaat tcatttttca attttaaatt
    19801 aactttcaca ttttttcaat aaatcttccc atcttctatc aacttctttc tgtatgcttt
    19861 caagtaaacc actgttttga ggtaaaagta agtgtctgaa tcttccctgt aatttaaacc
    19921 attcacttac cggttttttc tcttttggtt tgtaagtgag tttatattta ccttctacaa
    19981 cttcatataa tggccagata catgtttcaa ctgcaagccg tgaaatttcc atcgtcagtt
    20041 caggttcatg aggccagcca agacgacatg gtgataaaac ttctataaat ttaaaaccat
    20101 taatttttac tgctttttca accttattta caaggtcatt ccatcttcca ggtatagaag
    20161 ttgcagcata cggaattccg tgtgcaacca taatttcaac aagatttttc tgccactgaa
    20221 ttttaccggg aattactttt ccaacaggag aagtagttgt atgagcatat ttaggagttg
    20281 ctcctgacct ctgtatacct gtattcatat atgcctgatt attgtaacaa acatataaaa
    20341 cattatgacc tctttcagca gctccactta atgcctgaaa tccaatatca tgagttccac
    20401 catctcctcc aaaaccaaca aatacaatat ctttatcaat tttcccttct cttttaaaaa
    20461 cattgtatgc tgcctctatt ccacttattg ttgctgctga attttcaaat gcagaatgta
    20521 tccatggaat tttccatgct gaataaggga aaattgtaga tgcaacttca acacatcctg
    20581 tggcacagga agcaataaca ggtttatcaa tagcaagtag aacaagtcgc ataactattc
    20641 ctgctccaca accagcacac aatctgtgac ctgaaataaa tctactttct cttttcgcaa
    20701 gttctcttat acttggcatt ttttcctcct tttttttcga aggaccgtcc tccgaattat
    20761 tcccttactc ctatatattt taatgtaaag tcctttttcc ctttttccag aatttttagt
    20821 aaatcattaa aaattatttc aacatcttca atctttacat ctcttccacc aagtccataa
    20881 acaaaatcag ttaaaatagg ttttttttca aggtcatata aacatgcaga aatttcagaa
    20941 tataatggac cacaaaaagc attcatacct tctactctat ccataattcc aacaactttt
    21001 aaatttttta atgaattttt gatatcctca actggtaaag gccttaaaac ccttattttc
    21061 aaaagtcctg cctttattcc tttttttctt aaactatcaa caacttcttt tcctgtccca
    21121 gcagttgaac caataacaac aattcctata tctgcatctt caagtttata attttccaca
    21181 aattcataat atcttccaaa tctgtctcca aattcctttc ctatttcaag tataactttt
    21241 aaagcatttc tcatcccttc tgcctgctgt cttttatgtt caaaataata gtcctgtaaa
    21301 tcaagtgctc ctattgttat cggtttccca tcaatcaatg cctttatcag attttctctt
    21361 tcaggtggat ttccaacaaa ttcctgaact tcttcgtctg aaagcatctc cattgtttca
    21421 aggcaatggc ttaaaataaa tccatcagtc ataatcatac agggtaatct aacttcagga
    21481 tgttcagcaa ttcttactcc ctgtattaaa ttatcatatg tctcctgaac attttctgaa
    21541 taaatctgta tccatccact atcccttgct ccaaaagtat ctgaatggtc gcagtgaata
    21601 ttaataggtg aggaaagtgc tctattgaca actgccatta ttattggtaa acgaaatcca
    21661 gaagcaatat agagcatttc ccacattaaa gcaagtccct gtgaagaagt tccagtcatt
    21721 gctcttgcac ctgctgcact tgctccaata caggcactca ttgctgaatg ttcactttca
    21781 acctgtataa attctgtttt aacacgtcca tcagcaacaa attgagaaaa aatctggaca
    21841 atttcagttg caggtgttat tggatatgca gcaacaacat ctgggtttat ctgtctcatc
    21901 ccttctgcaa atgcttcatt ggctgttttc gctactttta ttcccatttt catctccctt
    21961 tttaattttt tcttccattg tttgaagttt taactttaag ttatttaaat tgtttatgat
    22021 gttatctata tctttctgcg ctttttccac ttctccaagt atgcttttat aagttgttat
    22081 ttccatttct ttttcaatca gttgtttatt caatttttcc ttctcattct taaaaatcaa
    22141 atattcagca cctgaaaaaa tccatcctga taaaagtaaa acccctaata aataaatttt
    22201 ctttttcatt tttccccctc atccttcatt tctattgctt ttttaggaca ttcataggcg
    22261 catattccac aacctttaca gtaatcataa ttaaagtgag accatttccc tttttctgaa
    22321 tcaacgacaa tagcatcatc aggacaactt atccagcata taaaacaatt aatacatttt
    22381 tcaggaatat gaacaggttt tttgcttctc caatttcctg ttttaaaatt taaagaacta
    22441 cccccttcaa gtatatctcc aactggtaat tctttccatg tcaacttttt ttccatttca
    22501 atctcctttc acttcattat atgctctttc tattgctttc agatttcctt caataacttc
    22561 tggtttattt ctgaatttca cctgcaattt tttttctgta tctttcaaca attcttcaag
    22621 tgtaagtatc cctgtaacct tcacaagagc acccatcatt ggagtattcg gtatattcct
    22681 tccaattgtc tccattgcaa tttttgaagc atcaacagtg tataccttcc ctttaaaatt
    22741 tatctttttt ttaatttctt ctggagataa aggagagtta atgagtaaaa taccttcttt
    22801 ttcatcaaga ccttcacaaa cattaatctg ttctacaagt gaaaaatcaa gaaccacgac
    22861 aacatcaggg ttttcaatac cagagtgaat tcttattgga gaggaactta ttctgttaaa
    22921 agaagcaaca ggtgctccca ttctttcagg tccatattca ggaaatgcct gtatatattt
    22981 accagttgaa atacaagcat ctccaaaaag aagtgctgct gtttttgctc cctgacctcc
    23041 tcttccatgc catcttatct caaaaatttc tttcattttt taactccttt gtatacaaga
    23101 aaaactgttt taattgtcaa aatttcaaga aaagaaacta aaaataataa aataattttt
    23161 aagaaagtta ttttatcata tttttgaaaa attgtaaaga aaaaaaccca gaaaaccaaa
    23221 aacggaaaaa ataaatttat aagttctgat aatttgtttt tcatgaagtc ataacttttc
    23281 ttaattccct caaaaattgt ctcgttttcc gctaaaatta taaaaggggt ataaagagtt
    23341 aaaaaaatat aaaaaaatgg tagtgcagcc cacacaacta ttataaagaa aagaaaaaaa
    23401 gaaaaaaatg aaagtatttt gattgaagaa attgcactta aagcaattaa ataaataatt
    23461 ccagcaagca aacttataag aagattataa agtaaaatag atgggaaaaa ataaaatccg
    23521 tccctgaata tatcagaaaa agtaaatgtt tcatcataga cacatttttt aattccagca
    23581 tatactgaaa ttgtaaaata aatttcaaag aaaaaaagaa aaaaatagta ggttttaaat
    23641 ttttccagta aaaaacttct caaagaaaca tataaaaagg ttataaaaaa aagaaacaac
    23701 tcctgctttt ttattttaat cagattttta taaatttcca tttttacttt ttaatcatac
    23761 atcctgaaaa acttctggtc aatagagttc cccttttgtc tcaaaatgtt tgaagtaaat
    23821 cttaaaaatg atataattaa aaatgaaaaa ggaggatatc atgtatagaa tagggaaaga
    23881 agaagtaaag gcagttgaaa aagtaataaa aagtaaggag ttattcaggg ttaaaagtag
    23941 aaaattgagt gagtgtgata agtttgaaaa ggaatgggct aagaaaattg gaactaaata
    24001 tgctttactt ttaaatggag gaggaactgc tgcattaatt tgtggacttg taggatttga
    24061 tattggacct ggagatgagg ttttaattcc atcatatact tttatggcaa ctgcaactgc
    24121 agttcttatg gttggagcaa ttcctgttgt ggttgaggtt gatgaaacat gtacaatgga
    24181 cccgatagat ctggaaaaaa agataacaaa aaatacaaag acaattattc ctgttcatat
    24241 ggttggattt ccttgtaata tggaaaagat aatgaatatt gcgaaaagat ataatttaaa
    24301 agtacttgaa gattgctgtc aggcagtcgg tggaagtttt aaaggtaaaa gattgggtac
    24361 atggggagat gcgggcgctt acagttttaa ttatttcaaa gtaataagtt gtggtgaagg
    24421 tggtggtctt gtaacagatg atagaataat ttacgaaaag gcaagtatat ttcatgatag
    24481 tggaactgct ttcagaccct atgctggtga attttcaatt cctatttttc ttggactaca
    24541 attcagagca agtgaaatta tgggtgcaat aatgagagtt cagttaaaac ggcttgataa
    24601 aatactgaag gatttgagga aaaacaaaag aagaataatg gaagaactta ttgattatcc
    24661 aggaattaag tttattcctt caaatgatat agaaggagat tgtggaatta tgattggttt
    24721 tagatttgat gacgaaaata ccgcaaaaaa gtttgttgaa attgctggtg gctacagacc
    24781 tattgattca ggaaaacatg tatggtttga ctggaagccg gtaattgaaa agagaatatg
    24841 gcataaggaa agattgaacc catataattt ccctgaaaat caaaatttaa gacatactta
    24901 tacaaaagaa atgtatccaa aatcaataga gaatttaagc agaactgttt taatttctgt
    24961 caatcctgac tggaaagata aagatataaa ggaaaaggta aaaaaaataa aaaaggcagt
    25021 ggaagaagtt tataaaaaat gaatattgaa ttttttgatg taaatgtctg gtttggaaat
    25081 ttgaaagatg atataaagaa aatatctgtt tctttaaagc gaatctctga aaaacttgat
    25141 gagttgaata taaaaaaggc gatagtttat catatttccc agagagatgt ccatcctgaa
    25201 ttcggaaatg aaattttaat aaaagagatt gcagataaag aaaaatttta tggaataatt
    25261 acacttttac catttcaaac agacgaaata aagaaaatag attttaaaga tttaaaaaat
    25321 aaaaaaattg ttggtttcaa tttttttcca aagaaacata actatatttt agataaaata
    25381 acttttggtg attttctttc tgaacttgaa tataaaaatt ttcctgtttt ttttgattta
    25441 ttaagtggtt tttctgttga ttatagagat gtttattcaa ttttaaataa ttttccaaaa
    25501 ttaacctgta ttttatgtaa tcttggtatc tggaatacaa atcgttttac atggccatta
    25561 attgaaaaat gcccaaatgt ttatcttgaa tcaagtttac tttctttaca ggaaggtgga
    25621 attgaggaaa cagttaaaaa atatggagca gaaagaattg tttttggaag cggttttcct
    25681 gaaaggtatt ttgaggcatc aattctacaa cttgtacatg cagaaataaa tgaaaaagat
    25741 aaagaaaaga ttgcacataa aaatatagag agaataatta aaaggataaa atatgaataa
    25801 actctggaag gaatttttaa aaaagggaaa gttagaagat tgtcctgtaa tagacatcca
    25861 ctgccatatg ggactttttt atggaagtca tatgccttat actgaccctg ataccatggc
    25921 aaaaagaatg gaactggctg gtgtaaaatt aattgttttc tgtcataatt atgcacttct
    25981 ttatcctgag gaagggaata agtacaatat agaagcagtt aggaaatatc ctgaaaaatt
    26041 taaagcatat tgcggaataa atccaaatta tcctgaagta gtagagaaag atttaagtac
    26101 atttgataaa tacagagatg tatatgttgg tttgaaactt ttacctgatt atcataaaat
    26161 acccctttca gataaaaaat ataaacctgt ctttgaattt gctgataaaa ataaacttat
    26221 tgtcttaact catacatggg gtagaagtat ttatgatgga gcggatgagg tggaaaaaat
    26281 attaaaaaaa tataaaaact taattcttat acttggacat tctattcatg gtgattggga
    26341 aaaagcaatt gaatttgtta aaaaatatga gaatgtttgt cttgaacttt gtgctattct
    26401 tgatgaaaga gggataattg aaaaatttgt ttatgaaatt ggttctgata ggatactttt
    26461 tggtactgat tttccatggt ttaatcatca ttattatata ggtgctttac ttgggagtgg
    26521 aatttcagaa gaggattgta gaaaaatact ttatttaaat gctaataaaa tcttaaagat
    26581 ttttgaatga taaacttcct ttctgctata atatttttga ctattggagt aattgtgtat
    26641 aactcaaact ataaagttat atttaagcat gacgatttaa ttactgtaaa tgaagggaaa
    26701 tgggaaagcg atttttttcc tgtaaaagag tttaattttt ataaattaac ctttacttca
    26761 aaatcaccct ataaaaattt ctgggcaatt attttttatg ataaaaataa aaatcaatta
    26821 atagcagata attattcaag ttttgataaa agtacggatt ttactgaaaa cactttttat
    26881 tttagaggaa aatgtaatgc taaatatgga aaagtaattt ttcaaaaaat tgaaaggaat
    26941 aaaattccat ttgaaataaa aaatatttca atcaaaaatg ctactgaaaa ggaaatttta
    27001 tactggatgg aaaatttata caaaaaaatc ccggaaatta aaaaatttac tataaaaaag
    27061 aattatatac ctgaaacaat gaaaaaactg aaaaaaggag aaaatttaag gattgtaatg
    27121 cttggagaca gtatcataaa tgatacggga aattcttttt ttgatatcct tcttaaaaaa
    27181 atatatccaa aagcaaaaat aaaaattata acatctgtaa gaggaggaac aggttgctgg
    27241 tattatcagg aagaaaacag agttaaagag tatgttcttg attataatcc tgatttactt
    27301 ataattggtg ggataagtaa taaaaatgat acagaagcga taagaaatgt tgtaaaacag
    27361 gtaagagaaa aaaagccaga cacagaaata attattatga gtaaagcggt tggaaaagaa
    27421 ggagacccaa gaactaatcc cgaatggaca tttgaaatac cggatgatga gagttcttac
    27481 agatatagat taaaaaaact ttcagaagaa gaaaagattg aattttttga tattgaagca
    27541 tattggggtg aatatataaa aaattcaaat ttaccttatg atgtattttt aagagaccct
    27601 gtacatgcaa atgaaattgg caggattgtt cttgcttatc ttttatattt ttatcttata
    27661 gaaacagatg ctgaaaaata aaattgaaaa agagaaattt tacaaaatta tgatgatttg
    27721 aaaactaatt aaaattatga tatcttaaag ttaagaaaga aggtgggaaa atggttgaat
    27781 atattaaatg gctcggtcat gcttctattt taatctcaat tggtgaaaaa aatatttatg
    27841 ttgacccatg gaaacttaaa aaagaccaac caaaggcaga tttaattctt attactcatt
    27901 ctcattatga ccattttagt cctgatgata tcaataaaat tataagaaat gatacagtta
    27961 ttattggacc gcatgatata ttaaaaatca aaacaggaga aaagatagaa attaaaccag
    28021 gacaggagac tgatttaaaa tgggttaaaa ttaaaggtgt tccagcatat aatattaata
    28081 aaaattttca tccaaaaagt aataactggt gcggatatat tattgaaatt ggaggaaata
    28141 aaatatacat tgcaggtgat actgacttta ttccggaaat gaaaaatata gttgcagatg
    28201 tggttatatt acctgttgga ggaacatata ctatgactgc agaagaagca gcaaatgcag
    28261 taaatataat tagaccaaaa atagcaatac catatcattt tggagacatt gttggttctg
    28321 aaaaagatgc aaaatttttc tcttctttta ttttaaattc agaagttaaa attttaaaaa
    28381 ttgaaaaata attttattta tcaaaaataa cttctttgcc tgtatctgaa cttatataca
    28441 ttgcttcaag aatttttatg acttcaagac tctcttctgg tttaactggt acttctttat
    28501 cctcttttac acattccaga aatactctta actcttcatg atggggagca acatttggta
    28561 aaacatctaa tctaagttca atatcctgaa gaatcccatt cttctccttt gcaagttttc
    28621 catttggcca tcttattcca ccttcagttc caaataaagt tgttgaaaaa tcatctattt
    28681 tttccatatt tgcaagaaaa ctgcaggtta aagttaaagc cctgttatca gaaaacctta
    28741 caaatccaga agcaaaatcc tctacattat atttttctct atcccactct ccccaaagcc
    28801 ctcttatatc ttttctcttt gctagttttg ttaaagttat accctttaca gaatgaactt
    28861 ttggacttcc catgaaccag taagttaaat caagaatatg tactcctata tcaaacattg
    28921 gaccaccacc tgaaagtgct ttatctatga atgtaaaact tgttggaaga tgtcttcttc
    28981 tcaaagcata tgcaattgca aaataaattt ctccaagttc tccatcatct atcagttttt
    29041 tcagagcaac cgactctttc ataaatctca tatgttgtgc agccataagt tttttacctg
    29101 atttttttgc ctgctcaatc attccttcaa cttccttctt gcttacagca agtggttttt
    29161 cacatattac atgctttcct gcttttaaag atgctattgt tgcaggaaaa tggactgtat
    29221 tcggagcaca gatatcaata atatcaattt cttcaatttt caacaattct tcataatttt
    29281 caaaaacata ttttattccc atttcattct ttgcaatatt ccttacattt tctgatggat
    29341 cagaaattcc aataacttca acatcggaca actttttcca tcctggataa tgtgcccatc
    29401 ttgcaattcc tccaaaacca ataaaaccaa ctaaatattt accatttttc attccttgcc
    29461 tccttgtttt taaatttcaa aattaaatct gacatcaaca gcaatacaac ctttttcttt
    29521 tactattaaa ggatttatat ccatttcttt tatctctgga aaatctgaaa caagttggga
    29581 tactcttaat aatgtctctt taactgaatt tatatccact tttggaatat ttctgaaacc
    29641 ttttaaaatt ttacttgttt ttatttcatt aatcatatta tctacatcta tatctgtaac
    29701 tgggattact ctgaaagata catcttttaa aagttcaaca taaattcctc caagaccaaa
    29761 cattattaaa tgcccaaatt gattatcttt attcactccc acgattacct caacaccttt
    29821 ttcaatcatc tcctgaattt gaactcctgt aatttttgca tctggtttat atctttttgc
    29881 tgaatttatt atatctaaaa atgctttttt tccttcctca ggagttctta tatttaactt
    29941 aattccacct gcttctgttt tgtgaattat atcaggagat acaagtttca ttacataaac
    30001 accttctttt tcttttaaaa atttctcaca ttcttcttca ttttttgcca gaaaagaatt
    30061 tataacatta atcccgtata atgcaagtat ctctcttccc tcaacatctc ctattacatt
    30121 tatatgttgc tttttaaaat aatctatttt ttctttaacc ttctcctttt gaactttaaa
    30181 ctcaacaata cttccttttg atttttcttc aattcttttt ttataatcaa acattgcttt
    30241 aaaagaaact atacctcttt caggagaaaa ataatttgga attttatttt ctctcaaaat
    30301 cttaattcct tcttcaaccc ttttcccacc cataaaacag gcaattactg gtttatcata
    30361 tttttttgat atttttacaa tacactctgc tgtctccttt atctgggttg ttacctgagg
    30421 agttaaaata gccataatac tatcaacatt ttcatcggaa atcacacact caattgcctt
    30481 ttcatatcta tctgcctgtg catctcctaa aacatcaaca ggattatgaa tatttgaagc
    30541 aggaggtaaa aaactcttta gtttttctat agtatcattt gaaaactctg caagtttaag
    30601 tcctgtttct tctatcatat caacagccat tactgatggt ccacctgcat ttgtaacaat
    30661 accaattcta ttccctttta taactggttg atatgaaaaa atcattgcaa aatcaaaaag
    30721 ttcttcaacc gtatctgctc ttattatccc tgactgtttt gttgctgcat taaatgaagt
    30781 atctgaacct gataaagaac ctgtatggga agttgctgtt tttgttccac tttctgtctt
    30841 tcctgatttt acaacaatta ttggcttttt actgctgact ctttttgaaa tatcaataaa
    30901 cctcctgcca tcacttaccc cttcaagata aagaagaata acatttgtat cagggtcatt
    30961 ttcaagttct tcaattaagt cagattcaga aacatctgct ttattcccaa aacttataaa
    31021 tttagaaagt cctatatttt ctttaactgc ccagtccaaa acagcagttc caagagcacc
    31081 tgattgagta ataaaagcaa tctttccttc tggtggcatt tcaaaagcaa aagaagcatt
    31141 taacttattt tttgtatcta taattccaag gcaatttggg ccaataagtc gcattttata
    31201 tcttttaaca atttctttta atttttcttc tctttttttc ccttcttttc ctgtttcttt
    31261 aaatcctgct gaaataacaa taagtccttt aactcctttt ttaccacatt gttctgctac
    31321 atcacaaaca aattcagatg gtattacgat aatggctaaa tcaacagaat cgggtatttc
    31381 tgaaacattt ttataacatt taagatttag aattgtatct gcttttggat ttactggaaa
    31441 aattttacct tgatatccac catctataag attttttaaa attttaaatc caagtttttc
    31501 tggattagtt gaagcaccta tcaaacacgc actttcaggt tcaaagaaat attttaacat
    31561 tttttctcct ttttaaatta atttcaattt aaggaaaaca ttataaacct taaaatattt
    31621 tttttcaaat cagatagaat ttactcgtat cttaatgcct gtattgggtc aagatatgct
    31681 gctttccttg caggataaat accaaaagtt attcctacac caacagaaat tgtaaaagaa
    31741 agagcaattg accaccatgt tataacagtc gtcataccag caaattttgt cacaagttta
    31801 ggtattaaaa taccaagtaa tatccctata aaaccaccaa ctgaagataa tataactgtt
    31861 tcaagtaaaa attgacttat tatgtccttt ctctttgcac caacagccct tcttatacca
    31921 atttctcttg tcctttcagt aactgtagca agcataatat tcataattcc aataccacca
    31981 actataagtg aaattgcagc aattgaacct aaaactatat tgaaaattct ttttgtatgt
    32041 tctgcctgaa gcaagagttg taaaggaaca agaatttcat aatcatcttt tttgtgattt
    32101 atttttaata tattttcaat tatcgcagaa gttgtcaaaa tcttatcggt attattcact
    32161 cttactataa atctatggta cttaacccag tttctctccc attttgaaac aggcccttct
    32221 tttgaagcaa tttcaaactc gccaaaataa gaccttcctg ttgtaaacgg aatatataca
    32281 tcaaaattaa catcttcagc agcaaatcct gttgttcctg ttgtaactcc cttttctttt
    32341 gcaacaccta ttactttaaa actgtaattt cttattgtaa ttgttttgcc tatggggtct
    32401 tctgttggaa ataaactttt tctgatagaa taacctataa cagcataggg ttgtatcaat
    32461 tcaatatcaa tctgtgttaa aaatcttccc atttcaagac caatattcat aacatcttta
    32521 tattcaggat atgtacatac aatccttccc ttaatacttt tttccatata tcgcatttct
    32581 tctttcattt cccatgtagg aactataagg tcaactgaag ggatagtttt tatcatctta
    32641 taatcatttt ctaaaagacc gtaaacactt acaaatgttg tagcttcctg ggttattctt
    32701 gtttctggtg gttttacact tctcacaata atattttgac tcccgagttc ttttattttt
    32761 tcccttgctt caaaacttgc tccctctcct attgcaagca tagcaattac tgatgcaact
    32821 ccaaaaacaa tgccaagaat tgttagaagt gaccttaatt tatgaagaaa tatatttttc
    32881 acaccaattt taataatcct taataaatag tagattttac ctctcatcta ttatttctcc
    32941 atccaaaatt cttaccaccc tctgaccata ttcagcaatc tttgggtcat gagtaattat
    33001 aacaattgtt ctgcccattt catttaattt tatgaatatt tccattattt cttttcctgt
    33061 ttttgtatca agatttcctg ttggttcatc tccaagtaac ataactggtt catttacaag
    33121 tgcccttgct atcgcaactc tctgctgctg gcctcctgaa agttcagtag gtctgtgata
    33181 cattctatca ccgagaccta caagatttgc ataatatttt gccttctcaa ctatttcttt
    33241 ctcttctatc cctctataaa tcaaaggaag accaatattt tcaattacat tcaactgtgg
    33301 aataagatta taattctgaa aaacaaagcc aattttttta tttcttattt ctgaaagttt
    33361 gtcatcatca agttgattta tatttacact gtcaagaaaa tactctcctt ctgtcggttt
    33421 atcaagacat ccaattatgt ttaaaagagt tgtctttcca cttccagaag gtcccataat
    33481 tgaaatatgt tctttctctt ttactgtaag atttacattt tttaaagcag gaacttttac
    33541 ttctccaagg tcataaattt ttttaacacc tctcattatt attattgaat tattctcatt
    33601 actcattttt taatttcttt ttcaggtgtt tttgtttcta ttttcccctt ctctgccatt
    33661 tcttcttttt tgactgtccc ttttgttggt cctttataac ttaaaacaac aacatctcct
    33721 tcttttactc catcttttac ctcaaccatt gtttcactac tttcgccaag tttaacattt
    33781 cttctgacaa tatttttccc ttcaagtacc tcacaatatg cctgtccttt ttcaaaatta
    33841 actgcaacaa ttggaattgc tataacattt tttaattctt ttaagagtat ttcaacatct
    33901 gcattcatac caggtctcat tgctttatcc ttatctatca attccacctt tactacataa
    33961 acatttacat caggatttaa tgattttaat gattggtctg gcatatttga aatctgaaca
    34021 actttacctt taaaaacctt atttgggatt gaagaaattt taacatttgc aatctggtct
    34081 aatgaaattt tctgaatgct tgcttcgtga actttaacat caacacccat tgaattaaaa
    34141 tcaggtaata ccagtaattc ttgtgattgc caaacagttg ccccaggttg tatctgaaca
    34201 ccttgaatca atgctctgaa actacctgca gaagttgaat atgtaacaaa accaggagca
    34261 gttgcaacta tagtacaatt ttttatattt gtttcaaggt cagtaagtcg tttttcgttt
    34321 aacttatatg cagccctttt actttttaaa tctgattctg cctgaattaa tcttgatttt
    34381 gcttttgact ttgttctatc aagttccgca actgcttctg tatagtttga taataattgt
    34441 tctaaatttt taggaaattc ataattaaca aaaagttgaa atgataattg tgcctgttca
    34501 agattaactc ctttttgttt taaagataac tggtctgctt caagttcatt tttggtaata
    34561 taaccctttt cagatagttt tttactccat tcaagttcgt tctgtgctct tataacttct
    34621 tcttttgcaa ggtcaatatt attttcaagt tgtcttttct tatttaatgc ctctcctcca
    34681 agtttttcac tgtttataat ctctttataa tcaatatttt ctcttttatt ttttttctct
    34741 atcacttcat ttgcaagaga agcaccaagg tatttctcaa ggtccatctt tgcaaatgta
    34801 acttttaatt ctgcttcttt tatcatacta tcattttctt ttttaacaat ttcaaggttt
    34861 tcctgtgcat ttaaataact tgcataactg ttttcaactg ttatctccat ttggtctctt
    34921 tgagtttcaa ggtcatcaga atcaagtttt accagtacct tccctttctt aacatcttct
    34981 tcagtaattt cagtcccttc atcaacaact tcaaggattg ttcttcttcc aggaacctga
    35041 tttgtaattt ttgttgattt taaagcaaca agatttcctg attcatgaat tgttataggt
    35101 aaatcaactt ttttaacttt atatgtcact atgtcttttg attgtttatc tgttgaattg
    35161 aaaaaataaa aaacagaaat taaaatgagt attaaaataa agcccaaaaa tattctattt
    35221 ttaaaaggtc ttaagtattt catttatatc tcctttccat ataccatttt catcaatttc
    35281 aagtttttct gttgtgtaaa gaaaatttaa atattcaagt ataaaactta caattgaatt
    35341 aataacatta ttttttgcag ataaatatgc atcctgtgct tcaagcaggt cccttgttgt
    35401 tgctcttcct gcctgaagta ataaatctgt actttctacc cttctttcag caagtttaaa
    35461 actattcaat tgaattatat aactctgata actttcttca agatttctgt aactatcaat
    35521 tatttctttt tttactgtat ctactttttt atcaaatgac ctttttgctc tttcatattg
    35581 gataagtgct cttttatatt catttctctg aggtaattta ttaaaaggaa tgttaaaatc
    35641 aattcctaaa ctatactgag gtgaatttaa atcaatattt ggcctatctt ttatatctcc
    35701 tgaactcttt atagaagcat tcatatttat tgaatttttc agattattaa gggcaatttc
    35761 aactttcctt tttgaatcct ccactttttc atattcagtt attaaatcaa gccggttctc
    35821 caaagcatat tttataaatt cttctgtatc aattttaact tcagacaatg gcttttcaaa
    35881 taattcttca atttctttat cagttaaaat taaattatat gatggagata atccgagttt
    35941 cattttgaaa gaatcaagag aactgtaata aagattttgt gcatctatcc acctctgata
    36001 ggcagtatat tcattctgtc ttgcctggtc aacctgaaaa gcagcaattc ttcctgcttc
    36061 tgaaagcatt tcaattcttt ctcttgtatc tttaagattc atataattag tataatagtt
    36121 ttccattctc tttttataaa gaaaaagatt taaatagtcc ctcgttattt ctattgaaaa
    36181 actcttctga taccttaaaa aatctcttat ttgatataca gtatttcttt cactttgaat
    36241 taaattttca agcgctattt ttcttcccgc tcctttaaaa agtggttgaa atagattaag
    36301 tgaaagaatt gactgaaatg ctttctcttt atctcctgtt aaatattgta aaattgcttc
    36361 acctatatta aaagttactt ctccagcagt agcaagtgtt tgaattaaat ttaaattcag
    36421 atttgaattt attgtttctt catcagattt actccagttg atattacctg ttaatgttat
    36481 tctgggttta aataaatatc tctgatatgt taaatctaaa actgaaagat agacattttc
    36541 ttttgctgtc tgatattctc tgtttttgtt tgaagcaaga attaaagcat ctttcaatga
    36601 taaaataact gtctcttttt gagtatcctc acttaaattt acaggcattt tatccttttt
    36661 tactttttcg ctttttttat taagaatttc ataaacttct ttatcagcag atttctcata
    36721 atatttcggt gaacaggata caagtaaaac catgctaaat ataaataaaa tgttcctttt
    36781 catgaataat ctctctttcc attaaagtat ttagacagag tttttaacaa taaagtttag
    36841 tatattttaa aatctatttc agtcaatctt aacaggtttt ttctcctcag atgatttata
    36901 tattgcatca ataattttca ttaaaattat tgcttcctct ggttttgaaa atggttcttc
    36961 ctgtcttctt attgtattca caaaatttat aactgctctt tcccttccca ttttagggtc
    37021 tggttcaact ataatttttc tatttactgg tttcccgtat tccattgtat aaagttcaca
    37081 atcatcaatt gcagtatcat ctattccatc ccttccaaaa agtcgtctta tcataccacc
    37141 tgcctttgtt ccctgaaatg taaccgatac ttcttccctt ttattcatct ctgcccaact
    37201 atttcttgca aaaagaacct ggtctgtttt aaatgtaata aaagcatgtg aacttgtctc
    37261 aacatccatt attccacctt caacatcagg tattccccac ggacctttaa aatcaggatt
    37321 acctgaaaaa tcattgaatg tttttgcaag aacaaaatca ggttctggaa aacctataaa
    37381 atataaggca aggtcaatca tatgaagaag gtcaataaca gggcctcctc ctgaaagaga
    37441 tttttgagta aaccatccac caaatccagg aataccacat cttcttatcc ataatgcctg
    37501 agcagaattt atttttccaa catctccatt ttttatgtat ttcataagtg cctgtgcttc
    37561 tggtcttgct ctattattga aatcaaacat taaaactttt tttgcatctt ttgctgtttt
    37621 taccattttt tctgtttctt ttgcatttaa agcaggtggt ttttcacaga atacattttt
    37681 cccattttta agacattcta ttgaaacctc tgcatgatat ttatttggaa cacagatact
    37741 aacaatatca atttcttttt ccttttcaag catttcttta taagaagagt aaacattttt
    37801 tatattgaat ttttctgctt tctgctttgc tctttctgta tcaatatcag aaattgcaac
    37861 aacatcaact ccacttttta aataacctga aatatgataa tcagcaat
//
LOCUS       DAOSAT010000006        34828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_905_length_34828_cov_5.062491, whole genome shotgun sequence.
ACCESSION   DAOSAT010000006 DAOSAT010000000
VERSION     DAOSAT010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 34828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34828
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_905_length_34828_cov_5.062491"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..669
                     /locus_tag="PLF90_01020"
     CDS             94..669
                     /locus_tag="PLF90_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOK55899.1"
                     /translation="MEKFIASLLSLFITLDPVGTIPFYVSYTHNFTKERKNRILFISI
                     SICFVVGVLFLYFGEFLFKFLKISFSDFLIASGIILLIFSITEFIGASQFKVEEEELA
                     IVPLAIPLLAGPAFLTTILISKKFYGTGITLICLFLNTVFAYFILFYSQNISHAIGKT
                     GIKGLIKILSLFLAALGIKFIREGIEQIIKF"
     gene            682..1359
                     /locus_tag="PLF90_01025"
     CDS             682..1359
                     /locus_tag="PLF90_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOK55900.1"
                     /translation="MEILKDFKIEVNKEYLIERLKIEKGSEDENEFLKLIGKALEIGR
                     PKAVYIEGFIDEKLEEAVIINGVKFESKILRKNLENVEKVFVFIATCGKELDRLNFKE
                     DILKNYWWDTIKEYFLGMARRYLFDYLKERYFLKDLIVMSPGASEKYVWPIEQQRELF
                     SLFGDVENLIGVKLTESFLMVPNKSISGFLFSSEKDYRSCKICRRKNCPGRTVEFDEN
                     LWKIYQK"
     gene            1369..2766
                     /locus_tag="PLF90_01030"
     CDS             1369..2766
                     /locus_tag="PLF90_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK55901.1"
                     /translation="MSEKKYNERFDRYITAMRNEMPDRIPIRPFVAEFTAKYAGYTCQ
                     EVTHDYRKAFEAVIKCGRDFDWDAMVPNMVYVWTGLTQALGLKYYAIPGIDIPSDTGF
                     QYIEPPEENAYMKPDEYDRLIDDPTEFLFNVWLPRISKDIGENNSYRGKLALVKGAMA
                     IFQYFNDLGAQVKRMKEEISMPSAISGILKAPLDIIADKLRGYIGLVMDLHTQPDKVL
                     KACEVLAPHLAKIAIMTGDPEKKLPVAFWMHRGCVPFINMEQFKSIYWPTLKPIIEEL
                     WRNGYQTLFYAEGNWDAHLDSFSELPEKSIIYHVDRGDIFKVHKKIGNKFCISGGIPN
                     ALLAVGSEKEVRDYCKKIIDNVASEGGYIMDASAIIQNDAKIENIRVMTDFTREYGVY
                     SQGHSRNYEEKKETYVPESKLKELKIRPGVCIPWEEKIKEIKNIKGDEDLIKKVWEEI
                     DTFGYIFIWQILLSF"
     gene            2826..2898
                     /locus_tag="PLF90_01035"
     tRNA            2826..2898
                     /locus_tag="PLF90_01035"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2859..2861,aa:His,seq:gtg)
     gene            3042..4364
                     /locus_tag="PLF90_01040"
     CDS             3042..4364
                     /locus_tag="PLF90_01040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK55902.1"
                     /translation="MNKEREIIKGLRWSVAAGAFNSIFCLLTVFGSIFLLFLSELSIP
                     KYKIGILLSFLPFFGIIAPFLSSYVDYFGSKRTFLICFGIRKFVIVSLLLLPLIIEKY
                     GIKTGINFVTFVILLFAFLRAFGETGYYAWTKEFIPDELRGKYTAIFNIIFNLTGILA
                     VSFGSYIVGKGEGIEKYLFLIGIAFIFGILSVYFMSFVPFGEPKREKPDINLKKILIP
                     LQDKNFLYFLFACGIALFGSSLMSFVPIFLKEKLNFIPRTIIFLDNASILGSILMGFI
                     WGYLGDRFGGRPVVILSLCLYILVPFFWLVLPRDIPSIFNFAFLLYFLSGGTLLGRSV
                     GDSRFLYAGILTNVNVIYYTSIYYAWIGIFQGFSPVFAGYILEKFKNLGILIFNRTFD
                     IYNIIFLINLLCQISAIIIYRKVKPDKDVKTRELFLKMRRRFFDLYKY"
     gene            4481..5734
                     /locus_tag="PLF90_01045"
     CDS             4481..5734
                     /locus_tag="PLF90_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK55903.1"
                     /translation="MKKLKVGIFGIKRGSSFANLFSINPNTEVVAICDYDELFLRNFL
                     KDKEGITGYSGYDKFLEHDLDIVVVCNYCTEHAPAAIKALDSGRHVLSEVIACKTLAE
                     GVDLCRAVERSKKVYMFGENYCFFPYIQEMERLYKEGVIGEYLYGECEYVHDCRGIWH
                     IITGGKEHWRNWLPSTYYCTHSLGPIIKITKTRPLKVVGFVVPNKLSREVGRIGDDWG
                     LFVCIMDNCALTRVIAWSTGPHDSIWYRVHGTKGAMENNRWKDTGVLNLHTVELTETL
                     DIKEEKRSYIPEFIKYKEEAKKAGHGGSDFFVVWSFVDSIIKDEPPPIDIYTAMDMTL
                     PGILAYRSACSGSIPLEVPDFRKEEERKKYENDRWSPDPKDRNIPGQPYTSILGEIKI
                     HDEIYRKVDEKRRKYLMDIEEKIKK"
     gene            5731..6303
                     /locus_tag="PLF90_01050"
     CDS             5731..6303
                     /locus_tag="PLF90_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK55904.1"
                     /translation="MKQLRMVLPKSKNFERIEIPDGYEIRNFERGDEEEYISLLSNNE
                     LGEWNLEKLQKTILDNTLSPEGIFFCVYSNKIIGTACGLDKGFYNNRKIAELGWVCVD
                     KNHRGKGLGYFLCFYVIQFLRKRKYEIIYLLTDPFRIGAIKTYFKLGFVPVIESEEDF
                     SRWDEVSKNLNLKIDYFLKSMDLFKFEKIG"
     gene            6330..7586
                     /locus_tag="PLF90_01055"
     CDS             6330..7586
                     /locus_tag="PLF90_01055"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOK55905.1"
                     /translation="MKIENEELKGIIDLWDLKKEIKKEIFKLLDENPALSPAHLFRYY
                     VPEGKKDKVGQFEKEFAEKFGSKYALAVNSGTSALIASLIACGIGPGDEVIIPGYTFF
                     ATCSAVLVAKAIPVIAEIDETLTIDPDDIEKKITKYTKAIMPVHMRGLPSNMEKIMKM
                     AKKYNLKVIEDCAQADGGSYKGKHLGTFGDAGCFSFDFYKIINTGEGGMVVTDDEKIY
                     IRAQSWHDTAACWRPNRYARERIPGELFYGENYRMGQINAAIGLAQLKKLDSYLKKMR
                     KNKKMLIESIGEVKRVKYAPVNDPEGECATNLVFLTERAEEAKKLMENLKKEKVPAGG
                     IYDKDIRDWHIYKYWEHIIEKKTPTEEGCPFTCSYYKGKLPSYSEDMCPNTLDILSRV
                     LIIGISENWKYKDIKEIAKKIKKYIN"
     gene            7748..9028
                     /locus_tag="PLF90_01060"
     CDS             7748..9028
                     /locus_tag="PLF90_01060"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOK55906.1"
                     /translation="MKVGTGKVDITPPVGVELAGYGPFLKRKSAGVHDNLFCRLIYLE
                     KEKEKGIIAGCDLICFKNNLVKKIRRKISKKFGIEKNKILLAGIHTHSGPATGGHIGW
                     GEEDEDYLSKLPELIVEAVRISKENLKEIKVGIGKIKIDGEKISYNRVKEDVGVNNEI
                     KVMKFVEKETEKPIVLLVNYGAHAVVQGPNNTLISRDWPGKAVDIIREKTQFETIFFQ
                     GACGDINSKPAVSKNPEDGFKEIERIGKYFAEVVIEGVDKIETEETNFFRNRSFKVIL
                     PNVPISKSELKKVIEENEKILEEEKDERKRNMAKFQIEGAKVQLSLYEKKGSLYHTEI
                     EIQLIEINKALLIAIPAELFVEFQRKIESAFPEKNVFVITYANGSIPYIPSRYDFQVK
                     GYASTFVPRIFGVMPFREDVGDVLVDSIVKFVRE"
     gene            9537..10301
                     /locus_tag="PLF90_01065"
     CDS             9537..10301
                     /locus_tag="PLF90_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK55907.1"
                     /translation="MEFFDIKSGIGVFSKPTGYTEPEELLRIMKNYSIKKSLVYHALS
                     REISPVEGNKILIEKIKEFENLIPVYGLLPRNSGDIENFERYIEKAIKDGVKSFLITY
                     NTFKIPLSHYLYSETLEILNEFKLPVIIDPTLPFSNWQVDSGDWQSIRLICEKYPDLP
                     VIFTEFRTRYHIRIVIDFIKNYKNFYYDVGSCWNYRVVEKLVGIKNGENLVLGTNLPF
                     SEPGQSLGMILCSDISESIKKKIAYGNIEKIIVKNE"
     gene            10294..11064
                     /locus_tag="PLF90_01070"
     CDS             10294..11064
                     /locus_tag="PLF90_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK55908.1"
                     /translation="MSKLKEKFLKSEKLEVELYDIHAHIGGYNRDYPVFDGESRKIIE
                     EMDRLNIKKAIVMPLGVYGTEWFYQNEKLINDCKKYSERLVGFCLVNLNYPEKVIGKE
                     ITRCRDEGIRGIKLIASYQNCEDTDKKTEYVCEYANHYKMPILNHNWGNPDFLEKMVK
                     KYQYIQYICGHFSFIWKDVVNNYENVWLCTCNYLGFGTVEKMVNEIRNDRICWGSDFS
                     DLHFGFTLGPILLSSISDEIKIKIISENTKNLIERYCI"
     gene            11078..12886
                     /locus_tag="PLF90_01075"
     CDS             11078..12886
                     /locus_tag="PLF90_01075"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOK55909.1"
                     /translation="MGKSIIEVVKKKNVKCSPVDIEDVKVDGFWKYVMERNREISIPN
                     LYKLFVQYKTVENFEIEAGLKKGEKTDRLATDSDLYKWIEGVSWDLQNKWDEKRVKHL
                     DKLIEIIKKAQKKSGYLNTATYIKKEFENLYSHHELYCGGHLIQSGIAHYRSTGKENL
                     LNIAIKWADYICKKFGKGKIEKTDGHPEVEMALVELYRTTKEEKYLKQAKFFLEIENP
                     YYSGLPNIPFLKFSELTGHAVRMMYLCSGATDYYIETGDKRYLKVLENLWKNLVLRKI
                     YITGGIGSRYEGEAIGVPYELPNLRAYCESCASIALMMWTFRMFLIKGECEYFDIFET
                     TLYNSFLASISFDGKKYFYVNPLSSIGNHERKEWYRTTCCPPNIQRFISSLPGCFYSI
                     SDDGVYVNLYDTSTANLKLNTGKRIKIEQRTGYPWKNKVLISVNSEAIFNLYLRIPKW
                     SDKTEVKIGEKIYNVENGKYFKIEAKDKTKIEINFDIRGEFYSGNPNIESVRNCFAIK
                     RGPLVYCLESIDNPDINLFNSLLWEQELKENYEEIFGGIITLNGETLTSDEELPLYEK
                     NKKIKINYKKKRFKAIPYYLWANRGRSQMNVWFLKK"
     gene            complement(12889..13425)
                     /locus_tag="PLF90_01080"
     CDS             complement(12889..13425)
                     /locus_tag="PLF90_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55910.1"
                     /translation="MGKKLFFLILTGFLSSVFSQNKKLEEASLNILFNFTAEKYEEVF
                     NSFYLPENYDEKNFKNDKEAITKGLEFIIEKLGDIRNFSKVNSTEKEIIALSLKTGPF
                     EEIENLKSYLSIYKVNFEKFGNAYISFEFIEKKEEYFLKKIEFLFPIQNNKSLSINKE
                     IQQFFIDLALKQKKTDKE"
     gene            13511..14146
                     /locus_tag="PLF90_01085"
     CDS             13511..14146
                     /locus_tag="PLF90_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOK55911.1"
                     /translation="MEKKIEKQLSLPFKNALQMSLRNFKIRFGRAIIVTMSILLGIAF
                     FMSVMASNVFTMALIEKGPENIRTLLVENLAELKVRQTWLVTLSLLVCTVGIINSMLM
                     AVTERSREIGTMKCLGALDRFVMELFLLEAMIHGIGGSIIGSFLGILIMTLVYVIQYK
                     KIILSIFPFLPLLKYVGISIILGTGLAIIGAIYPAIVSARMEPAEAIRREV"
     gene            14161..14901
                     /locus_tag="PLF90_01090"
     CDS             14161..14901
                     /locus_tag="PLF90_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020266656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK55912.1"
                     /translation="MPREVIVRTIELKKTYMLGKIPLHVLKGIDLEILKGEYISIMGP
                     SGSGKTTLFNMIGGLDKPSEGKVYIDEVDIAQLDAYELAWLRCRKIGYIFQTFNLIPV
                     MSALENVMLPMIFAGMTTDDAREKAKSLLELVGLGERLHHKPFELSGGQQQRVAIARA
                     LANDPAIILADEPTGNLDLKTGKEIINLLRQMNKEKQVSIITATHDLKMLDVSDRVIW
                     IRDGMIERIEDRENLDIRVGHVEGEEAG"
     gene            14933..15721
                     /gene="proC"
                     /locus_tag="PLF90_01095"
     CDS             14933..15721
                     /gene="proC"
                     /locus_tag="PLF90_01095"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOK55913.1"
                     /translation="MKLGIIGFGNMGSAIISGILEKKVIKKEKIFVFDVDKEKIKDIP
                     ANVVENNKEIVENSDLIIIAVKPKDAENVLCEIKNYLNSEKILISIMAGIKIKRIEEI
                     INKKIPIIRTMPNLNVKVKSGIIVYCSNKYGKKYEDIVKKIFAPCGIVLKMPENKFDT
                     ITAISGSGPGFLFYIAEEFKKICIEKGISSKILKDLISYLFYGTGKMLVDTKIEPGKL
                     KEMVSSPGGTTIAGLSIFEKRKFGDILREVIDAAEKRSKELSSN"
     gene            15730..16197
                     /locus_tag="PLF90_01100"
     CDS             15730..16197
                     /locus_tag="PLF90_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55914.1"
                     /translation="MIRIKPHHFVDIVRDYGKGIKEYTPHPYGHALHIIAKKILGDLD
                     IIIQIELGIDDICEPCIHNVNGVCDDLIDTSFRPQAPESKNEWNLLIDKRWCNVLNIK
                     QEDLIGIKELCKRIKNCSYSDIKNIYRELPENMIVEREKNIKKGLELILNKKF"
     gene            16270..17937
                     /gene="ilvB"
                     /locus_tag="PLF90_01105"
     CDS             16270..17937
                     /gene="ilvB"
                     /locus_tag="PLF90_01105"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOK55915.1"
                     /translation="MKGAQIFVECLKREKVEVIFGYPGGAVLPLCDVLYDSGIRFILT
                     RHEQGAAHAADGFARSSGKVGVCLATSGPGATNLVTGIATAYMDSVPIVAITGQVATH
                     LIGNDAFQEVDITGITRPITKHNYLIKDINEIPKVVREAFYIAKTGRPGPVLIDFPVD
                     IQRKEGDFYWPEKIEMRSYKPVYEGHPLQIKKAWELIKNSKRPVLIAGGGVIISNASD
                     ELKKFVEKTNVPVAFTLLGLGSLDVEWEYSLGMPGMHGTKYANYAIVESDLLISVGCR
                     FDDRVTGKIDEFAPHAKIIHIDIDPSAISKNVEVDVPIVGDAKHVLKKLNEMAEKLDI
                     DEWLKKIKEWKEMYPLKYERNGLKPQYIIEKISEYTKGDAIICTEVGQNQMWTAQYYK
                     FKKPRTLITSGGLGTMGYGFPASIGVKVANPDKIVIDIAGDGSIQMNIQELATAVYNK
                     IPVKVFILNNCYLGMVRQWQQLFYNRRYAFTCLEGGQPDFVKLAESYGAIGYRVTTEE
                     EFNKILPEVMEINDKVIFVDCRVEPEENVFPMVPAGASLKQILDGLA"
     gene            17976..18461
                     /gene="ilvN"
                     /locus_tag="PLF90_01110"
     CDS             17976..18461
                     /gene="ilvN"
                     /locus_tag="PLF90_01110"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOK55916.1"
                     /translation="MESHIISVTVENRFGVLARIAGLFSARGYNIDSLCVAETEDPSI
                     SRMTIVVKGDEKILEQIYKQLNKLIDVIKVQDLTKEDHIERELALIKVNTSNFNERQE
                     VMQLTNIFRAKIVDVGKKTLTIEISGSGSKIRAMLDLLKGFGVKEIIRTGKIAITREF
                     K"
     gene            18477..19469
                     /gene="ilvC"
                     /locus_tag="PLF90_01115"
     CDS             18477..19469
                     /gene="ilvC"
                     /locus_tag="PLF90_01115"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOK55917.1"
                     /translation="MAKIYYDKDADLKFLKDKVVGIIGYGSQGRAHALNLRDSGLNVI
                     VSELEGTPNYEKAKKEGFEPISADQLTKKADVIMILVQDNVQPFVYKSQIAPNLSKGK
                     YIGFAHGFSIHYSQIVPPKDVNVFMVAPKGPGDLVRDQFTKGQGVPCLVAVYQDSSGD
                     TLNIGLAYAKGIGGTRCGVVETTFKEETETDLFGEQVILCGGATALIKAAFEILVENG
                     YQPEVAYYETCHELKLIVDLIIERGIQGMRQVISDTAEYGDMTRGPRVINESVREEMK
                     KILKEIQTGEFAREWILENSVGRPVFNALRRQSEEHLIEKVGKKMRDMMPWLKK"
     gene            19495..20046
                     /gene="thpR"
                     /locus_tag="PLF90_01120"
     CDS             19495..20046
                     /gene="thpR"
                     /locus_tag="PLF90_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOK55918.1"
                     /translation="MRTFIGIDLPDDIKENLKEVVDRLKKIREAKPVKIENLHITLKF
                     LGEVEEKDIEEIKKKLEICANKFKSFGVEIKGIGVFPSEKRIRVLWIGAEDEGYLKKL
                     NNEIEEEMSELGFEKEKEFVSHITVARFKSVPNLNFIREISEKYNNFLFGKFRVESFN
                     LYESKLLPEGPIYKVVAKFQLPQ"
     gene            20133..21167
                     /gene="recA"
                     /locus_tag="PLF90_01125"
     CDS             20133..21167
                     /gene="recA"
                     /locus_tag="PLF90_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOK55919.1"
                     /translation="MDKEKALEMTISQIEKEFGKGSIMRLGEKRLIEVEVIPTGAISL
                     DIALGVGGIPRGRVTEIFGQESSGKTTLALEILAQAQKQGGTVAFIDAEHALDPNYAK
                     ALGVNIEDMLVSQPDTGEQALEIAEALIRSNAIDVVVIDSVAALVPRAELEGEMGDSV
                     MGLQARLMSQALRKLTGYISKSKTAAIFINQVREKIGVFFGNPETTPGGRALKFYASI
                     RMEVRRIDSIKRGNDIVGSRIKVKIVKNKVAPPFKETELDIYYGQGVNKESSLIEAAL
                     NYGILEKKGSYFSYEGKTLGQGIDNIIENLKKNPALYKEIEKKIKEKVGLIKTEIKTE
                     KKEGKDDERS"
     gene            21154..22320
                     /locus_tag="PLF90_01130"
     CDS             21154..22320
                     /locus_tag="PLF90_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015842742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK55920.1"
                     /translation="MKEVNVALIGYKFMGKAHSFAYKNVSLFFDLPVKVVMKVICGRT
                     EKAVSQAKEKFGWLEYETDWKKVVERKDIDLIDITTPPNAHKDIAIEAAKNGKIVFCE
                     KPLASTLKDAYEMLEAVEKYGVKHAICFNYRKLPAIGLAKKLIEEGKIGKIYHMRAVY
                     LQDWIVDPDFPLVWRLQKQVAGSGAHGDLNAHLIDMARYLVGEFDEVVGINETFIKER
                     PVLKAKEELETGLGEFKEKAEEKGIVDVDDATIFLVRFKNGAIGSFEATRFAPGRKNG
                     QRFEINGSKGSIVFNLERLNELEYYSRDDESGTQGFRTIIATDASHPYAANWWPAGHI
                     IGYGEAFVNMVADIFNSISKGENPSPNFYDGVKCQEVLEAVDQSIRERKWIKVK"
     gene            22367..23848
                     /locus_tag="PLF90_01135"
     CDS             22367..23848
                     /locus_tag="PLF90_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOK55921.1"
                     /translation="MKKNIMIGFIFGLIFSGLVFTGYSAIEKKTESKKFYSGIITPTS
                     LDEPLTLQSAFVQVAKSVKPAVVQITTEKIVTYRYWDPFGDLDEFFRSPFEEFFGTPR
                     RRQQPKTYQKKQEGLGSGFIVDEDGYILTNNHVIEGVDKILVKLLGDERKYEAKVIGT
                     DPKTDLALIKINAGKPLPYVKLGDSDKIEVGEWVIAIGNPFGLEETVTVGVVSAKGRS
                     GFGITQYEDFIQTDAAINPGNSGGPLVNIRGEVIGINTFIIAPYVAQGVGFAIPINLA
                     KKVFTQLKEKGKVTRGYLGVYLQALDEELAKSFGLSEIKGALVAQVLKDSPAEKGGIK
                     DGDVIIEYDGTEVKDVKDLQMKVANTPVGKRVSVGIWRDKKKIVLNVTVGEMPAEEPV
                     AAETEEKLWRGMRVSALTEEMKSQFGIDENEGVVVTYVETGSPADESGIKVGSVIKMI
                     NDYKISNISDYRKVIKNIPKNTDVRVQIKFGDRTRFLVLKGEK"
     gene            23866..24267
                     /locus_tag="PLF90_01140"
     CDS             23866..24267
                     /locus_tag="PLF90_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOK55922.1"
                     /translation="MKNEELIKKILKLLSKKDYSEEEIREKIPSITPSILKELKKKNL
                     IDDYSLSLKIIENLQNKGKGYHYIFKELERRKIKDQIIEKLKKEYDFEKEVEICKKVV
                     SRMEGKSISSIILNLKSRGFSEEIIEKVINF"
     gene            24347..26938
                     /gene="alaS"
                     /locus_tag="PLF90_01145"
     CDS             24347..26938
                     /gene="alaS"
                     /locus_tag="PLF90_01145"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOK55923.1"
                     /translation="MESKKIRESYLKFFEKKGHKIVKSSSLIPLEDPTLLFTNAGMVQ
                     FKKFWATDIPLPYTRATTCQKCLRAGGKDSDLEKIGESGKHHTFFEMLGNFSFGDYFK
                     KEAIEWAFEFVIDVLKIREENLWVSYFKDDIETKDYWKKFLPEDKLIPLGEKDNFWGP
                     AGETGACGPCTEIYFDFGKDKGCGREDCKPGCDCERFLEFWNLVFPQYDKQKDGAFLP
                     LKRKGVDTGMGLERISRILQGVETNYDTDLFVPIIKKIEEISGFSYKENKRNFRIISD
                     HIRGIVFAISDGIFPSNEGRGYVLRRIIRKAVFSGLDLNLKEPFLFNLSEVVIRKMSE
                     IYPELMENKKVIEKVIHEEEERFLSLISSSRKIFFEIVKDQKEIKGETLFKLYDTYGI
                     PKDLIDELAFQYDKEIKWDEFDEYMVKQRELSRAKSKIGMKKKEILISDKILETDFSG
                     YEKTSDTGKLVGIYQDENDFFYLVFDRTVFYPEKGGQIGDRGIIYNDKFKFKVIDTQI
                     DEKGLIYHIGNVLNGGIDDLKIGDVFYLNVDEEFRRSVSINHTSTHLLHYALREIIGK
                     EVRQAGSYVGDDKLRFDFVCFNDIDEEIIEKVENLVWEKIFEKATVKVEEMTLNEAIE
                     KGAIALFTEKYGEKVRVVSTGDFHKEVCGGTHLKNTGDIFLFHITSFSTIGKNLKRIE
                     AVTYKKCLDYFSKSIDTFKNLSNLLKTDEGKIKDRIEKLLEEIKEKERLIEKYVNLNI
                     EKIVEEILKNPKKILKNGKEINFFFRSVDSVEKKDDIAKISDRITEKVKNSLVFLISE
                     SGEKKIFVLKTGEEIKNEISAIKIVKNLSKYIKGGGSEKFVSGIIEKNIKFEFIVDEI
                     IKILENE"
     gene            26943..27770
                     /locus_tag="PLF90_01150"
     CDS             26943..27770
                     /locus_tag="PLF90_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK55924.1"
                     /translation="MKKNQILEDERKIITRIRKGDKKLREEFIKKNQGLIIHIAKKYA
                     FSNEILPDLIAEGNMGLIRAIEKFDFRKKVKFGTYAYFWIKRFVLRALMKEFEILKIP
                     ERYHEFKEKMDEVNNAYNLKFGRDATDEELAKELKLPLNIVKKFKKYSEDLTIISSDY
                     YNGEKDVDLFELIDTTGKKTEKFWEILRNKDILDKIFERIKKKEKRANVDVWCKVLKL
                     HYGLENGIQYSYKEIAKKLGVTRQRVHQIKKICLKKLRKEWEEMKNEGFKEFNSDNP"
     gene            27733..28245
                     /locus_tag="PLF90_01155"
     CDS             27733..28245
                     /locus_tag="PLF90_01155"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOK55925.1"
                     /translation="MKDLKSLIRTIPDFPKKGILFRDITPVLQNAEAFRKVIMTFKKE
                     TPQDVTKVIAIESRGFIFGASLAYLLGVGFVPIRKSGKLPWKKVKMDYDLEYGTDTIE
                     IHQDAIEKGEKVILVDDLLATGGTALASCKLIEKCGGIVEKILFLVELTDLNGREKLK
                     NYDVFSIIKF"
     gene            complement(28269..29285)
                     /locus_tag="PLF90_01160"
     CDS             complement(28269..29285)
                     /locus_tag="PLF90_01160"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOK55926.1"
                     /translation="MRNYNVCVVGAGMVGKKMVEILIERNFPFKNLKILATKEREEEI
                     TGRIFKIEETKIESFKGIDIAFFAGTEGEKGASKLFGWEAVKMGCIVIDNGADFRMDD
                     RVPLVVPEVNPDALKKHQGFIANPNCSTIQMVVALYPLHLEFNLKRVIVSTYQAVSGT
                     GKKAVEELSKQVESYVKGERIVNEVYPYQIFGNVIPEIGSLSDEYEGYYTEEVKMIKE
                     TRKIMNLSDLPVSATTVRVPVFNGHSESITAQFEKEVDVEKAREILKNSSGIEVIDQP
                     EKSLYPVPLFVSGKDEVFVGRIRKNPALENSLDMWVVADNIRKGAALNAIQIAEKMIE
                     MGLI"
     gene            complement(29282..29857)
                     /locus_tag="PLF90_01165"
     CDS             complement(29282..29857)
                     /locus_tag="PLF90_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOK55927.1"
                     /translation="MIKKIWIKTWDKYFEIYRLIGLTEKILLSIIFSILTGLSAQIYI
                     KLPFTPVPLTMQTFTVLLSGILLGKNFGSLSQIFYFLGGISGIGWFYGGTSGILRPTT
                     GYLVGFIFASYIAGYFSEKKNKLSGMIFATLIIYFTGIIWLKFFVNKGLKDLIFIGVL
                     PFIPFDIIKAILAGLFAKNIFHLKDLQEEEK"
     gene            complement(29869..31137)
                     /locus_tag="PLF90_01170"
     CDS             complement(29869..31137)
                     /locus_tag="PLF90_01170"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOK55928.1"
                     /translation="MDWYEYKKENGVKLIYKRFPEFHSVAVGVFIKTGSRFENPEYAG
                     ISHFIEHLLFKGTKNRSCNEIKEAIEGVGGSFNGFTSAEGTCYWIKILDKYLNLSFDV
                     LSDMIKNPLLKEEDIEKERNVIIEEINMYRDIPAKYVHELFDTLIFGQHPLGMPIAGT
                     PETLTRIKREDIVDYIDKKYSGANIVVSVAGKFEVKEMKKLFDKFFDGIERKKENQFE
                     KWDKKDNGPKVKLLIKDTEQTHISFGGFTYSYQDERKYPLSILNVILGGNMSSRLFNR
                     IRENLGLAYEIRSFIRQYKDTGIYNISAGVSGENLEKAMVEIVKELKEIKEKGVKEDE
                     VERAKRFLISQFLMGLEDNLEYMLWIGEQKLLKEKMEKINEIVKKVEKVKKEEVEEIA
                     KDIYKKENSYLAVISKKGDETKLKEIIGEL"
     gene            31221..32096
                     /locus_tag="PLF90_01175"
     CDS             31221..32096
                     /locus_tag="PLF90_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK55929.1"
                     /translation="MRIGFMMSYDKERVEFAKKVGFKSCELRVGVNDDFFPGKDGWET
                     KAKEVVEYYKANDIRISCLAGFYVNHMDPEKEEDYKKLVRNVIILAEKMGVPVVAGFS
                     GRIVGKPLEESIQKFVEIWSEHAKFAEAHGVKIAFEHCPMGYFHTPCNGINFMSTPKM
                     WEIGFSEVKSEAIGLEWDPSHLICQFIDPVITIRDFGKRIYHVHAKDAHINWDIVKKY
                     GIWHHGAIEHCFPGLGDTDWRLCIKELIRAGYKNDLNIEGWHDAVYHDRVAKGKEDEG
                     LIIAFRHLSQFVVQD"
     gene            32187..33257
                     /locus_tag="PLF90_01180"
     CDS             32187..33257
                     /locus_tag="PLF90_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK55930.1"
                     /translation="MEKEKIFKTKLEVLKEKLLYEIFKKNPGEKLPTEHELAKIYGVN
                     RSTVNKVLSLLENEGLIERKTGIGSIIKEKNERRLNYRIGVIVERSSGHVYEYLTRLM
                     IKKIQDSRFFSLLVDLGEPTDKILSHLDEILENNPEFVIVDGIGYFPFWFLKENISKI
                     RNLIFINRFETDLKLNATYILSDYEKGGYIGTKYLFEKTGEKILIVTQSNPSYRPNID
                     EERLKGVKRAFEEYGIEFKDENLIVNKNEKYLKEAIRKIFEKEKEIRGIFTFADNVGR
                     QVQEILEELGMWLGVDYIMVGYFNTSHSIDFSPQLPSISINEKGLIEKLGEVLNTGFP
                     KEIFKVEPILIERKIREREVIS"
     gene            33254..33970
                     /locus_tag="PLF90_01185"
     CDS             33254..33970
                     /locus_tag="PLF90_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOK55931.1"
                     /translation="MKEKGFTLIELLVVIAIIAILAAMLLPALDQARKRAQIATCMSN
                     LKQIYVALSMYRNDYDKYFPTAYEPRANSCSASLTLLTGQYVYYNSPTKEGPDYLKNY
                     SLFICPASSRTQSPTGVADWMNCSYAYARNFAEKNEREPKKYAIMADLKGDWQYYDTG
                     KQANILCGYLIRNQSYMPNHSFDGVNILFLDGHTEFRGNTGTKKDQWGNRMSVLNLAD
                     ISNIATGNTDPYALFYPLGF"
     gene            33989..34780
                     /locus_tag="PLF90_01190"
     CDS             33989..34780
                     /locus_tag="PLF90_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOK55932.1"
                     /translation="MKRNGFTLIELLVVVAIISILAALLLPALNSARHKARLATCMNN
                     LKQIGMCMRMYIEDWEGFIPPKNDGVPINWLLESNIGTDKGGPSGNSNTPHRLALLRW
                     KYDYNLKKWVSSYATTNKLFFCPGIDYKRSKLYNYGNSYWMARNCGYSYSWRGASFWD
                     GPVLISYPISIKFDQIFNYKRYHPNGWLAWVACPRSDLIKDPGAPHQDTGVNVLYYDG
                     SVKFLPHRKEIVSNISVDYNTDGDAWLGQTYQFWYYAIDSYQKKF"
BASE COUNT        13043 a         4075 c         6655 g        11055 t
ORIGIN      
        1 tagagcggtg gacttcgaat ccaagggtcg ccagttcaaa tctggccagg cgcgcataac
       61 cgcaaggtta gaccttgcaa aagggagaaa atgatggaaa aatttattgc ttctttactt
      121 tcgcttttta ttacacttga ccctgttgga acaattcctt tttatgtaag ttatacgcac
      181 aattttacaa aagaaaggaa aaacagaatc ctttttatct caatttctat ttgttttgtt
      241 gttggtgttt tatttcttta ttttggtgag tttttattta aatttcttaa aatttcattt
      301 agtgattttc ttatagcaag tggtataatc cttttgattt tttcaataac agaatttata
      361 ggagcaagtc agtttaaagt tgaggaagaa gaacttgcaa tagttccgct tgccattcct
      421 ttacttgcgg gtcctgcttt tttgacaaca attttaattt caaaaaaatt ttatggtaca
      481 ggaataactt taatctgtct tttcttaaat actgtttttg cttactttat tcttttttat
      541 tctcaaaata tttcacacgc aatagggaaa acaggcataa aaggacttat aaaaattcta
      601 tctctttttc ttgccgcatt gggaataaaa tttataagag agggtattga acagataata
      661 aaattttaga aaggagagga aatggagata cttaaagatt ttaaaattga agtaaataaa
      721 gaatatttga tagaaagatt aaaaattgaa aaagggagtg aagatgaaaa tgagtttctg
      781 aaattgattg ggaaagcact tgaaatagga agaccgaagg cagtttatat tgaaggattt
      841 atagatgaaa aactggaaga agcagtaatt ataaatggag ttaaatttga aagtaaaatt
      901 ttaaggaaaa atcttgaaaa tgttgaaaag gtatttgttt ttattgcaac atgtggaaaa
      961 gaacttgacc gattaaattt taaagaggac attttgaaaa attactggtg ggatacaatt
     1021 aaagaatatt ttttaggaat ggcaagaagg tatttatttg attatttgaa agaaaggtat
     1081 ttcttgaaag atttgatagt tatgagccct ggagcaagtg aaaaatatgt ctggccaatt
     1141 gaacaacaga gagaactttt ttctcttttt ggagatgttg aaaatcttat tggtgtgaaa
     1201 ttaacagagt cgtttttgat ggtaccaaat aagtcaattt caggattttt attttcatct
     1261 gaaaaagatt acagaagttg taaaatatgt agaagaaaaa actgtccagg aagaaccgtt
     1321 gaatttgatg aaaatttatg gaaaatatat cagaaatagg gggttaaaat gagtgaaaaa
     1381 aaatataatg aaagatttga caggtatatt acagcgatga gaaatgaaat gccagacaga
     1441 ataccaataa gaccttttgt tgcggaattt actgcaaagt atgctggata tacatgtcag
     1501 gaagtaacac atgattacag aaaggcattt gaagcagtta taaaatgtgg aagggacttt
     1561 gactgggatg ctatggttcc taatatggtg tatgtctgga caggtttgac tcaggcactc
     1621 ggtttaaaat attatgcaat accgggtata gatattcctt ctgataccgg ttttcagtat
     1681 atagaaccac ctgaagaaaa tgcatatatg aaaccagatg aatatgacag attgatagat
     1741 gacccgacag aatttttatt taatgtatgg cttccaagaa tttctaaaga tattggagaa
     1801 aataattctt acaggggaaa acttgctctt gttaaaggtg ctatggcgat atttcagtac
     1861 tttaatgacc ttggagcaca ggttaagaga atgaaagaag aaatttctat gccttctgca
     1921 ataagcggta ttttaaaagc acctcttgat ataattgcag ataagttaag gggatatata
     1981 ggacttgtta tggatttaca cacacaacct gataaagttt taaaagcatg tgaagttctt
     2041 gctccacatc ttgcaaaaat agcaattatg actggtgatc ctgaaaaaaa actaccggtt
     2101 gctttctgga tgcacagggg atgtgttcct ttcataaata tggagcagtt taagtcaatt
     2161 tactggccaa ctttaaaacc aataatagaa gaactttgga gaaatggata ccagacactt
     2221 ttttatgctg aaggaaactg ggatgcacat cttgatagtt tttctgaact tcctgaaaaa
     2281 agcataattt accatgttga tagaggtgat attttcaagg tccacaaaaa aatcgggaat
     2341 aaattctgta ttagcggagg gataccaaat gctttacttg cagttggttc tgaaaaagag
     2401 gtgagggatt actgtaaaaa aataattgat aatgttgctt ctgaaggtgg atacattatg
     2461 gatgcatcag caataattca gaatgacgca aaaatagaga atataagagt tatgactgat
     2521 ttcacaagag aatatggagt ttattcacag gggcacagta gaaattatga agagaaaaaa
     2581 gaaacttatg tgcctgaaag taaactgaaa gaattgaaga ttagaccagg agtatgtatt
     2641 ccttgggaag aaaaaataaa ggaaataaag aatataaaag gggatgaaga tttaataaaa
     2701 aaggtatggg aagaaattga tacttttggt tatatcttta tatggcagat tcttctttct
     2761 ttttgaaaat agagttattt tttagtttac attttccaat ataaaggata aaatataaaa
     2821 gaatggtgag tgtagctcag cggatagagc actggactgt ggctccagga gtcgggggtt
     2881 caaatcccct cactcaccct aattttttca aaaacaaaag gtatttggtt aaaagtcagt
     2941 ttctaattac acatttcaat tttcactaac ctatcactct gcatattgtt aataaatttt
     3001 tgacttaatt tgaatcagtt gtattattat ttattttgaa aatgaataaa gaaagggaaa
     3061 taataaaagg tttaagatgg agtgtggctg caggtgcttt taattcaata ttctgccttt
     3121 tgactgtttt tggttctatt tttctacttt ttctatcaga actcagtatt ccaaaatata
     3181 aaatagggat tttactttct tttctgccat ttttcggaat tattgctcca tttttaagca
     3241 gttatgttga ttattttggc agtaaaagaa cttttcttat atgttttgga atcaggaaat
     3301 ttgttatagt ttctttactt ttacttccac tcattattga aaaatatggg attaaaaccg
     3361 gtataaattt tgtcactttt gttattttgt tatttgcatt tcttcgcgca tttggagaaa
     3421 ccggttatta tgcatggact aaagagttta ttcctgatga attaagagga aaatatacag
     3481 caatttttaa tattatattt aatttaacag gaatactggc tgtttctttt ggaagttata
     3541 tagtaggaaa aggagaagga atagagaaat atcttttttt gatagggatt gcttttattt
     3601 ttggaatttt aagtgtttat tttatgagtt ttgttccttt tggagaacca aaaagagaaa
     3661 aacctgatat aaatttaaaa aaaatactta taccattgca ggataaaaat tttctttact
     3721 ttctttttgc ctgtgggatt gctctttttg gaagttctct tatgagtttt gtacctatat
     3781 ttctcaaaga aaaactcaat tttataccaa gaactattat ttttcttgat aatgcgtcaa
     3841 ttttgggttc tatattgatg ggttttatat ggggttatct tggagataga tttgggggaa
     3901 gacctgttgt aattttgagt ttatgtctgt atatacttgt tccttttttc tggttagttt
     3961 taccaagaga tataccttca atttttaatt ttgcattttt attatacttt ctttcagggg
     4021 gtactcttct cggtaggtct gttggtgatt caaggttttt atatgcagga attcttacca
     4081 atgtaaatgt tatttattat acatcaatat attatgcatg gattggcatt tttcagggat
     4141 tttcacctgt ttttgcaggt tatattcttg aaaaatttaa aaacttggga attcttattt
     4201 ttaatagaac ttttgatatt tacaacataa tttttttaat aaatttgtta tgccagattt
     4261 cagcaattat aatatacaga aaggtaaaac ctgataagga tgtaaagaca agggaattat
     4321 ttttgaaaat gagaagaaga ttttttgacc tttataaata ctgagaaaat ttatactttt
     4381 ggaagaggaa tttgaaagat tttataagcg atgaaattaa gattaaaata ttctctgaaa
     4441 aagttttaga aaaaatttat tgaaagaaaa agggggaaag atgaaaaaac ttaaagtcgg
     4501 gatttttgga ataaaaagag gttcttcctt tgctaatctt tttagtataa atccgaatac
     4561 agaagttgtt gctatatgtg attatgatga attatttctc agaaatttct taaaggataa
     4621 agaaggaata accggttaca gtggttatga taaatttctt gagcatgacc ttgatatagt
     4681 cgttgtttgt aattattgta cagaacatgc accagcagca ataaaagcat tagattcagg
     4741 tcgtcatgtt ttatcagagg taattgcttg taaaactctg gcagaagggg ttgatttgtg
     4801 tcgtgcagtt gagaggtcaa aaaaagtata tatgtttgga gaaaattact gtttttttcc
     4861 ttatattcag gaaatggaaa gattgtataa agaaggagtt attggagagt atctttatgg
     4921 agaatgtgaa tatgttcatg attgtagagg tatatggcat atcataacag gtggaaaaga
     4981 acactggaga aactggcttc cttcaacata ttactgcact cattctcttg gacctataat
     5041 aaagataaca aaaacaagac ctttgaaagt ggttggtttt gttgttccaa ataaactttc
     5101 aagagaagtt ggaagaattg gagatgactg gggtttattt gtatgtataa tggataattg
     5161 tgctttaacc agagttattg catggtcaac aggtcctcat gattcaatat ggtatagagt
     5221 tcatggaaca aaaggagcaa tggaaaataa tagatggaag gatacaggag ttttaaatct
     5281 tcatacagta gaattaactg aaacccttga tataaaagag gagaagagaa gttatattcc
     5341 tgaatttata aagtataaag aagaagcaaa aaaagcagga catggtggaa gtgatttttt
     5401 tgttgtatgg agttttgttg attcaataat aaaggatgaa ccaccaccca tagatattta
     5461 taccgctatg gatatgacct tgcctggaat tcttgcatat cgttctgcct gttccgggag
     5521 tatccctctt gaagttcctg atttcaggaa agaagaggaa aggaaaaaat atgaaaatga
     5581 tagatggtca ccagacccaa aagatagaaa tatcccaggt caaccctata cttcaatact
     5641 cggtgagatt aaaattcatg atgaaattta tagaaaggtg gatgaaaaaa gaagaaaata
     5701 tttgatggat attgaggaaa aaattaaaaa atgaaacagt taagaatggt tttgcctaaa
     5761 tcaaaaaatt ttgaaagaat agaaattcca gatggatatg aaataagaaa ttttgaaaga
     5821 ggagatgaag aggaatatat ttctctttta agcaataatg aactcggtga atggaatctg
     5881 gaaaaacttc aaaaaacaat acttgataat actctctctc ctgaaggtat ttttttctgt
     5941 gtctatagta acaaaataat tggaactgcc tgtggtcttg ataagggatt ttataataac
     6001 agaaaaatag cagaacttgg atgggtatgt gttgataaaa atcatagagg aaaaggactt
     6061 ggttattttt tatgctttta tgtaattcaa tttttgagaa aaagaaaata tgaaattata
     6121 tatcttctga cagacccatt tagaattggt gcaataaaaa catattttaa acttggtttt
     6181 gtccctgtta ttgaatctga agaggatttt tcaagatggg atgaggtttc aaaaaattta
     6241 aatttaaaaa tagattattt tttaaaaagt atggatttat ttaaatttga aaaaatagga
     6301 taaaataaga agagaaaagg aggttaatta tgaaaataga aaatgaagaa ttaaagggaa
     6361 taattgattt gtgggatttg aaaaaagaga taaaaaagga aatttttaaa ctgcttgatg
     6421 aaaaccctgc actttcacct gcacatcttt tcagatatta tgtaccggaa ggaaaaaaag
     6481 acaaagttgg acaatttgaa aaagagtttg cagaaaaatt tgggagtaaa tatgctctgg
     6541 ctgttaattc cggaacgagt gctttaattg cttctttgat tgcctgcgga attggacctg
     6601 gtgatgaagt tattataccg gggtatacat tttttgccac ctgttcagca gtacttgtag
     6661 caaaagcaat tcctgttatt gctgaaatag atgaaacatt aacaatagac cctgatgaca
     6721 tagaaaagaa aataacaaag tatacaaaag cgattatgcc tgttcatatg aggggacttc
     6781 cttcaaatat ggagaaaatt atgaagatgg caaagaaata caacttaaag gtaatagagg
     6841 actgtgcaca ggcagatggt ggaagttata aaggaaaaca tcttggaact tttggagatg
     6901 caggatgttt ttcatttgac ttttataaga taataaatac aggagaggga ggaatggttg
     6961 taacagatga tgagaaaatc tatataaggg ctcaatcatg gcacgatact gctgcctgct
     7021 ggagaccgaa tagatatgca agagaaagga tacctggaga acttttttat ggagaaaatt
     7081 acaggatggg tcagattaat gctgctattg gtctggctca attgaagaaa cttgatagtt
     7141 atttgaaaaa aatgagaaaa aataagaaaa tgcttattga atctataggt gaagtgaaaa
     7201 gagttaaata tgctccagta aatgaccctg aaggtgaatg tgctactaat cttgtatttt
     7261 taaccgaaag ggcagaagag gcaaaaaaac ttatggaaaa tttaaagaaa gaaaaagttc
     7321 ctgctggtgg aatatatgat aaagatataa gggactggca tatatacaaa tactgggaac
     7381 acataattga aaagaaaaca ccaacagaag aaggatgtcc atttacatgt tcttattata
     7441 aagggaaatt accttcatat tcagaagata tgtgtccgaa tacacttgat attttaagca
     7501 gagttttaat cataggaatt agtgaaaact ggaaatataa agatataaaa gagatagcaa
     7561 aaaagattaa aaaatacata aattaaaaat ggagaaaaaa ggtttttata ttatatcttc
     7621 ttcaatttta aaagttgggg aaagatttac acttaaattt aaagttctga cactccatat
     7681 tttgttaact gaaaatcctg aaatggtgaa cttggctggc taactccagt atggaaagga
     7741 ggataaaatg aaagtcggta ctggaaaggt tgatattact ccacctgttg gagttgaact
     7801 tgctggatat ggtccttttc ttaaaagaaa atcagcaggt gtacatgata atcttttttg
     7861 tagattaatt tatcttgaaa aggaaaagga gaaaggaatt attgctggtt gtgacttaat
     7921 ctgttttaag aataatcttg taaaaaaaat aagaagaaaa ataagcaaaa aatttggtat
     7981 agaaaaaaat aaaattttac tggcaggtat tcatacccat tcaggtccag cgacaggtgg
     8041 gcatattggg tggggagaag aagatgagga ttatctttca aaactccctg aattaattgt
     8101 tgaggcagtg agaataagta aagagaattt aaaagagata aaagttggaa ttggaaaaat
     8161 aaaaatagat ggagaaaaaa taagttataa cagggttaaa gaagatgtag gggttaataa
     8221 tgaaataaaa gttatgaaat ttgttgaaaa ggaaactgaa aaaccgattg ttcttcttgt
     8281 aaactatggt gctcatgcag ttgttcaggg accaaataat acattaataa gcagggattg
     8341 gccaggaaaa gcagttgata taatcagaga gaaaacacaa tttgaaacaa ttttctttca
     8401 gggtgcgtgt ggagatataa acagtaaacc agcagtaagc aaaaatcctg aagacggctt
     8461 taaagaaatt gaaagaattg ggaaatattt tgccgaggta gttattgaag gagttgataa
     8521 aattgaaaca gaagagacaa attttttcag aaatagaagt tttaaagtta tccttccaaa
     8581 tgttccaata tctaaaagtg agttgaaaaa agttatagaa gaaaatgaaa aaatattaga
     8641 agaagaaaaa gatgaaagaa aaagaaatat ggcaaaattt cagatagaag gggcaaaggt
     8701 tcaactttct ctctatgaaa aaaaagggag cctttatcac acagaaatag aaattcaact
     8761 aattgaaata aacaaggcac ttttaattgc aatacccgca gaactttttg ttgaattcca
     8821 gagaaagatt gaaagtgcat ttcctgaaaa aaatgttttt gtaataacct atgcaaatgg
     8881 aagcattccc tacattcctt caagatatga ttttcaggtt aaaggatatg cttcaacttt
     8941 tgttccaagg atttttggag ttatgccatt cagagaagat gtaggagatg ttcttgttga
     9001 tagtatagtt aagtttgtga gagaataaaa aatgaaaaag taattctatt tgttgatact
     9061 ttaagggctg accacatttt ttattataag aggaatggat ttttacataa atctaacatt
     9121 ttatttcaga aaatattgtg aacctcactt gaacgcttac aaaccataca acaatcctaa
     9181 aaaatttaat aaaaattttc aatcataaac aaggtgattt tgatgatttg aaagttgaaa
     9241 aaacaacctc tagatatgaa tatgttttag gatgatgtaa aactggtaaa atacaggata
     9301 attatattta tcagactgct agagaagaag aaataacgat ttaactttta tgacgaataa
     9361 ataagatatt tttgattttc tttttttgag aagtaattaa aaaactgaaa gaaactggaa
     9421 ttgacaaaga aacgatgatt atgataaatt taatcataga gaacaacttg tatcgcagga
     9481 tttctttctg tattataggt cgcgtagtat atctatggat tgtaaaggaa tgaaaaatgg
     9541 aattttttga tataaagagc ggaattggag ttttttcaaa accaactggt tatacagaac
     9601 ctgaagaact tttgagaata atgaaaaatt attcaataaa aaagtcgctt gtttatcatg
     9661 ctttaagtag agaaatatca cctgttgagg ggaataaaat actgattgaa aaaataaaag
     9721 aatttgaaaa cttaattcct gtttatggtt tattacccag aaattcaggc gatattgaga
     9781 attttgaaag atatattgaa aaggcgatta aagatggagt aaaaagtttc ctgataacat
     9841 ataatacatt taaaattcca ctttctcatt atttatattc agaaacactg gaaatactca
     9901 atgaattcaa attaccggtt ataattgacc ccactttacc tttttcaaac tggcaggtag
     9961 attcaggtga ctggcagagt ataagattaa tctgtgaaaa atatcctgat ttacctgtta
    10021 tttttacaga attcagaaca aggtatcata taaggattgt tattgatttt attaaaaatt
    10081 acaaaaactt ttattatgat gttggaagtt gctggaatta cagagttgtt gaaaaacttg
    10141 tgggaataaa aaatggagaa aatttagttt tagggacaaa tcttcctttt tcagaacccg
    10201 gacaatctct cggtatgatt ctttgttctg atatttccga aagcataaag aaaaaaatcg
    10261 catatgggaa tattgaaaaa ataatagtga aaaatgagta aattaaaaga aaaattttta
    10321 aaaagtgaaa aactggaagt agaactttat gatatacatg ctcatattgg tggatataac
    10381 agggactatc ctgtttttga tggagaaagt agaaaaataa tagaggagat ggatagattg
    10441 aatataaaaa aagcgattgt tatgccttta ggagtgtatg gaactgaatg gttttatcag
    10501 aatgaaaaac ttataaatga ttgtaaaaaa tattctgaaa gattggttgg attttgtctt
    10561 gtcaatctga attatcctga aaaagtaata gggaaggaaa taacaagatg tagagatgaa
    10621 ggaataagag gaataaagtt gatagcaagt taccagaatt gcgaggatac agataaaaaa
    10681 acagaatatg tctgtgaata tgcaaatcat tataaaatgc caattttaaa tcataactgg
    10741 ggaaatcctg attttcttga aaaaatggta aaaaaatatc agtatattca gtatatatgc
    10801 gggcatttta gttttatatg gaaggatgtt gttaataatt atgaaaatgt atggttatgc
    10861 acctgtaatt atcttggatt tggaactgtt gaaaaaatgg taaatgaaat aaggaatgac
    10921 agaatctgct ggggttctga tttttctgac ttacattttg gttttactct cggtccgatt
    10981 ttgctttctt caataagtga tgaaattaaa ataaaaataa tttctgaaaa tacaaaaaac
    11041 cttatagaaa gatactgcat ttaaaataaa aaaagaaatg gggaaaagta taattgaagt
    11101 tgttaaaaag aaaaatgtaa agtgttcgcc tgtggatata gaagatgtta aagttgatgg
    11161 attctggaaa tatgtgatgg aaagaaacag ggaaataagt atacctaatc tttataaact
    11221 ttttgttcaa tataagacag tagaaaattt tgaaattgaa gcaggattaa aaaaaggaga
    11281 aaaaacagac cggcttgcaa ctgactctga tttatataag tggattgaag gagttagttg
    11341 ggatttgcag aataaatggg atgaaaaaag agtaaaacat cttgataaat tgattgaaat
    11401 aataaaaaag gcacagaaaa agtcaggata tttaaatacc gctacttata ttaaaaaaga
    11461 gtttgagaat ttatattcac atcatgaact ttattgtgga ggacatttaa ttcagtcagg
    11521 catagcacat tacaggtcta ctggaaaaga aaatctttta aatattgcta ttaagtgggc
    11581 tgattatata tgtaaaaaat ttggtaaagg aaaaatagaa aaaacagatg gacatcctga
    11641 agttgaaatg gctcttgttg aactttacag aactactaaa gaagaaaaat atttaaaaca
    11701 ggcaaaattt ttccttgaga ttgaaaatcc ttactattct ggtcttccaa acattccatt
    11761 tttaaagttt tcagaactca caggccatgc tgtaaggatg atgtatttat gttctggtgc
    11821 tactgattat tatattgaaa caggagataa aagatatttg aaagttcttg aaaatttatg
    11881 gaaaaacctt gttttaagaa aaatttatat tacaggtgga attggttcaa gatatgaggg
    11941 agaggcgatt ggagtacctt atgaacttcc gaatttaaga gcatactgtg agagttgtgc
    12001 atctattgct ttaatgatgt ggactttcag aatgttttta ataaaaggag aatgtgaata
    12061 ctttgatatt tttgagacaa cactttataa ttcatttctt gcttcaattt cttttgatgg
    12121 taaaaaatat ttctatgtca atcctttatc ctcaataggt aatcatgaaa gaaaagaatg
    12181 gtatagaaca acatgctgtc ctccaaatat acagagattt atttcatctt tgccgggttg
    12241 tttttattca ataagtgatg atggagttta tgttaatctt tatgatacct caactgcaaa
    12301 cttaaaatta aatacaggaa aaagaattaa aattgagcag agaacaggat atccatggaa
    12361 aaataaagtt ttgatttctg ttaattctga ggcgattttt aatttatatt taagaattcc
    12421 aaaatggagt gataagacag aagtaaaaat tggagaaaaa atttataatg tggaaaatgg
    12481 aaaatatttt aaaatagaag cgaaggataa aactaaaata gagataaatt ttgatattag
    12541 aggtgagttt tattcaggta atccaaatat agaaagtgtg agaaattgtt ttgcaataaa
    12601 aagaggtccc cttgtttact gtcttgaaag tatagataat ccagatataa atcttttcaa
    12661 ttctttatta tgggaacagg aattgaagga aaattacgag gaaatttttg ggggaattat
    12721 aactttaaat ggagaaactt taacaagtga tgaagaatta cctttatacg aaaaaaataa
    12781 gaaaattaaa ataaactata aaaagaaaag atttaaggca attccttatt atttatgggc
    12841 aaacagaggt agaagtcaaa tgaatgtctg gtttctgaaa aaataatctc attccttatc
    12901 cgtctttttc tgttttaaag cgaggtcaat gaaaaattgc tgaatttctt tattgattga
    12961 taaactttta ttattctgga taggaaataa aaactcaatt tttttaagaa aatattcttc
    13021 ttttttttct ataaattcaa atgatatata tgcatttcca aatttttcaa aattgacctt
    13081 ataaattgat aaataacttt ttaaattttc aatttcttca aaaggtcctg tttttaatga
    13141 taaagcaata atttcttttt ccgtagaatt tacctttgaa aagtttctta tatcaccaag
    13201 tttttcaatt ataaattcaa gaccttttgt tatcgcttct ttatcatttt taaaattttt
    13261 ttcatcataa ttttccggta aataaaaact gttaaaaact tcttcatatt tttcagcagt
    13321 aaaattaaat agtatgttta aagaggcctc ttctaacttc ttattttgag agaaaaccga
    13381 tgaaagaaag ccagttaaaa ttaaaaaaaa taatttttta cccattaaaa aaattataat
    13441 ataatttttc taaagtttta aacttttttg tattttttaa aaatggtata atttctaaaa
    13501 aggagcaaag atggaaaaaa agatagaaaa acaattgagt ttacctttta aaaatgctct
    13561 tcagatgagt ttaagaaatt ttaaaataag atttggaagg gcaataattg taacaatgtc
    13621 aattttactc ggtattgctt tttttatgtc tgttatggct tcaaatgttt ttaccatggc
    13681 tttaatagaa aagggtcctg aaaatataag aactttgctt gttgaaaatc ttgcagaact
    13741 gaaagtaaga cagacatggc tggtaactct ttctcttctt gtatgtactg ttggtattat
    13801 aaattcaatg cttatggctg tcactgaaag atcaagagaa ataggaacaa tgaaatgtct
    13861 tggagcactt gacagatttg ttatggaatt gtttttactt gaagcaatga ttcacggtat
    13921 cggtggttca attatcggta gttttctggg tattttaatt atgactcttg tttatgtaat
    13981 ccagtacaaa aaaataattc tatcaatttt tccattttta ccacttttaa aatatgttgg
    14041 tatttcaata atccttggaa caggacttgc tataattgga gcaatttatc cagcaattgt
    14101 ttcagcaaga atggaaccag ccgaagcaat tagaagagaa gtttaaaaaa ggagggaaat
    14161 atgccaagag aagtaatagt aagaacgatt gagttaaaga aaacatatat gcttgggaaa
    14221 ataccattac atgttcttaa agggattgac cttgaaattt taaagggaga gtatatttct
    14281 attatgggac catccgggag cgggaaaaca actttattta atatgatagg aggacttgat
    14341 aaaccatcgg aaggtaaggt ttatattgat gaagttgata ttgcacaact tgatgcttat
    14401 gaacttgcat ggctcagatg cagaaaaatt ggatatattt ttcaaacatt caatttaatt
    14461 cctgttatga gtgcacttga aaatgtcatg cttccgatga tttttgctgg aatgaccaca
    14521 gatgatgcaa gggaaaaggc aaaatcatta cttgaattgg taggattagg agaaagattg
    14581 catcataaac catttgaact ttctggagga caacagcaga gagtagcaat agcaagagcc
    14641 cttgcaaatg accctgcaat tattcttgca gatgagccaa caggaaacct tgatttaaaa
    14701 actggaaagg aaataataaa tcttttaaga cagatgaata aggaaaaaca ggtaagtatt
    14761 attacggcaa cccatgattt aaaaatgctt gatgtttcag acagggttat atggataagg
    14821 gatggaatga ttgaaaggat tgaagacaga gaaaaccttg atataagagt gggtcatgta
    14881 gaaggagagg aagccggtta ataaaatttt acctgcctta aaggagagac aattgaaatt
    14941 aggaataatt ggatttggaa atatgggttc tgctattatt agtggtattc ttgaaaaaaa
    15001 agttataaaa aaagaaaaga tttttgtttt tgatgtagat aaagagaaaa taaaagatat
    15061 ccctgcaaat gtagttgaaa ataataaaga aattgtggaa aattcagatt tgataataat
    15121 agcagtaaag ccaaaagatg ctgaaaatgt tctttgtgaa ataaaaaact atctgaattc
    15181 agaaaagata ctcatttcaa taatggcagg gattaaaata aaaagaatag aagaaattat
    15241 caataagaaa ataccgatta taagaacaat gccaaattta aatgtaaaag taaaatcagg
    15301 gataatagtt tattgctcaa ataaatacgg taaaaaatat gaagatattg ttaaaaaaat
    15361 ttttgctccg tgtggaattg tgttaaaaat gccagaaaat aaatttgata caataacagc
    15421 aataagtggt agtggtcctg ggtttttgtt ttatatagca gaggaattta aaaagatatg
    15481 tattgaaaaa ggtatttctt caaaaatctt aaaagattta atctcctatc ttttttatgg
    15541 aacagggaag atgcttgttg atacaaaaat agagccgggt aaattgaaag aaatggtatc
    15601 atctcctggt gggacaacta tagcgggttt aagtattttc gaaaaaagaa aatttggtga
    15661 tattcttaga gaagtaatag atgcggcaga aaaaagaagt aaagaattat ccagtaatta
    15721 gatatgtata tgattagaat taaaccccac cattttgtag atattgtgag agattatggg
    15781 aaaggtataa aagaatatac tccacatcct tacggacatg ctttacatat cattgctaaa
    15841 aaaattttag gcgatttaga tataataatt cagattgaac tcggaataga tgatatatgc
    15901 gagccctgta ttcataatgt aaatggagtt tgtgatgatt taatagatac ttcttttaga
    15961 ccccaagcac cggaatcaaa aaatgagtgg aatcttctta ttgataaaag atggtgtaat
    16021 gtgcttaata taaaacagga agacctcata ggaattaagg aattgtgtaa gaggattaaa
    16081 aattgttctt attctgatat aaaaaatatt tacagagaac ttcccgaaaa tatgattgtt
    16141 gaaagagaga aaaatataaa gaaaggactt gaattaattt tgaataagaa attttaattc
    16201 aatgttgttt tcttaatttg acttttttac aatgtagatt tattatatta aaattcaagg
    16261 agaaattaaa tgaaaggagc acagattttt gttgaatgtt taaagagaga aaaagttgaa
    16321 gttatttttg gttatccggg tggcgcagtt ttacctttgt gtgatgtttt gtatgatagt
    16381 ggaataagat ttattttaac aagacatgaa cagggtgctg cacatgctgc tgatgggttt
    16441 gcccgttctt ctggaaaggt cggtgtttgt cttgccacaa gtggacccgg tgcaacaaat
    16501 cttgtaacag gaattgccac tgcttatatg gactctgttc caatagttgc aattacagga
    16561 caggtagcaa ctcatttaat cggtaatgat gcttttcagg aagtagatat aacagggatt
    16621 acaagaccca taacaaaaca taactactta ataaaagata taaatgaaat tccaaaagtt
    16681 gtaagagaag ctttttatat tgcaaaaact gggcgacccg gtcctgtttt aattgatttt
    16741 cctgttgata ttcagagaaa agaaggtgat ttttactggc ctgaaaagat agaaatgaga
    16801 agttataaac ctgtatatga agggcatcca ttacagataa aaaaagcatg ggaactgata
    16861 aaaaattcaa aaagacctgt tttaattgca ggaggaggag ttataatttc aaatgcaagt
    16921 gatgaactga aaaaatttgt tgaaaaaaca aatgttcctg ttgcatttac attacttggt
    16981 cttggttctc ttgatgtaga gtgggaatat tctcttggta tgcctggaat gcatggtaca
    17041 aaatatgcaa actatgcaat tgttgagtca gaccttttaa tttcagttgg atgcagattt
    17101 gatgatagag ttactggaaa gattgatgaa tttgctccac acgcaaaaat aattcatata
    17161 gatatagacc cttctgcaat aagtaaaaat gttgaggttg atgttccaat agttggagat
    17221 gcaaaacatg tattaaaaaa attaaatgaa atggctgaaa aacttgatat agatgaatgg
    17281 cttaaaaaaa taaaagagtg gaaggaaatg tatcctttga aatatgaaag aaatggttta
    17341 aagccacagt atataataga aaagatttct gaatatacaa aaggagatgc aataatatgt
    17401 acagaagtcg gacagaatca aatgtggaca gcccagtatt ataaatttaa aaaaccaaga
    17461 actttaataa cttccggtgg acttggaact atgggatatg gttttcctgc ttcaattgga
    17521 gtgaaagttg caaatccaga taaaattgtt atagatattg ctggagatgg aagtattcag
    17581 atgaatattc aggaacttgc aactgctgtt tacaataaaa ttcctgttaa agttttcatt
    17641 ttgaataact gttatcttgg aatggtaaga cagtggcagc aactttttta caatagaaga
    17701 tatgctttta cctgtcttga aggaggccaa cctgactttg ttaaacttgc tgaaagttat
    17761 ggagcaattg gatatagggt aactacagaa gaagaattta acaagatttt accagaagtt
    17821 atggaaataa atgataaggt tatttttgtt gattgtagag ttgagccaga agagaatgta
    17881 tttccaatgg ttcctgctgg tgcttcttta aaacagattc ttgatggact tgcataaatg
    17941 gagggaaaat ggggaaaaat aataatgaaa ataaaataga atcgcacata atttcagtta
    18001 cagttgaaaa cagatttgga gttcttgcga gaattgcggg ccttttttca gcaaggggtt
    18061 ataatattga tagtttgtgt gttgcagaaa cagaagaccc ttctatttca cggatgacaa
    18121 ttgttgtgaa aggtgatgag aaaattttag aacagattta taaacaactt aataaactca
    18181 ttgatgttat aaaagttcag gatttaacga aggaagacca tatagaaaga gaacttgctt
    18241 taataaaagt aaatacttct aatttcaatg aaagacagga agtaatgcaa ctcacaaata
    18301 ttttcagggc gaaaattgta gatgttggta aaaaaacatt aactattgaa atatcaggaa
    18361 gtggttcaaa aataagagca atgcttgatt tacttaaagg ttttggagtt aaagaaataa
    18421 taagaacagg gaaaatagca attaccagag aatttaaata aaaaaggagg aaaacgatgg
    18481 cgaaaattta ttatgataaa gatgctgatt taaagttttt gaaagataaa gttgttggaa
    18541 ttattggata tggaagtcag ggaagagcac atgcattaaa tttgagagat agtggattaa
    18601 atgttattgt gagtgaactt gaaggaactc caaattatga aaaggcaaaa aaagaaggat
    18661 ttgaaccaat ttctgcagac cagttaacca aaaaagcaga tgttattatg attttggttc
    18721 aggataatgt tcagccattc gtatataaat ctcaaattgc tccaaattta agtaagggga
    18781 aatatattgg ttttgcacat ggtttttcaa ttcattacag tcagatagtt cctccaaaag
    18841 atgtaaatgt ttttatggtt gcaccaaaag gacctggaga ccttgtaaga gaccagttta
    18901 caaaaggtca gggtgttcca tgtcttgttg ctgtttatca agattcaagc ggagatacct
    18961 taaatatagg acttgcttat gcaaaaggaa ttggaggaac aagatgtgga gttgttgaga
    19021 caacatttaa agaagaaaca gaaacagacc tttttggtga acaggtgata ctatgtggtg
    19081 gagcaactgc attaattaaa gcagcatttg aaattctcgt agaaaatggt tatcagccag
    19141 aagttgctta ttatgaaaca tgccatgaac ttaaacttat tgttgatttg ataattgaaa
    19201 gaggaataca gggaatgaga caggttataa gtgatacagc agaatatgga gatatgacaa
    19261 gaggaccaag agttataaat gagagtgtaa gagaggaaat gaaaaaaata ttaaaagaaa
    19321 tacagacagg agaatttgca agagaatgga tacttgaaaa cagtgttgga agacctgtat
    19381 ttaatgcttt aagaagacaa tcagaagaac atttaataga aaaagttgga aagaaaatga
    19441 gagatatgat gccctggctt aagaaataat tcggaggacg gggtttggaa aataatgagg
    19501 acttttatag ggattgattt gcctgatgat ataaaagaaa atttaaaaga agttgttgat
    19561 agattaaaaa aaataagaga agcaaaacca gttaaaattg aaaatctgca tataactttg
    19621 aaatttcttg gagaagtaga agaaaaggat attgaagaaa taaagaaaaa acttgaaata
    19681 tgtgcaaata aatttaaaag ttttggtgtt gaaataaagg ggattggggt attcccttca
    19741 gaaaaacgta taagggttct ctggataggt gcagaagatg aaggatacct gaagaaactg
    19801 aataatgaga tagaagaaga aatgagtgaa cttggttttg aaaaagaaaa ggaatttgtt
    19861 tcacatataa cagttgcaag attcaaaagt gttccaaatt taaattttat aagagaaatt
    19921 tctgaaaaat ataacaattt tttatttggg aagtttaggg ttgaaagttt taatttatat
    19981 gaaagtaaat tattaccgga aggacctatt tataaagtag ttgctaaatt tcaactgcct
    20041 caatgatttc cactgcccac cttaaaggtg ggagagaggt ggttgactta aaaaaaagga
    20101 aagggtatta taatcaaaac ggaggggaga atatggataa ggaaaaagca cttgaaatga
    20161 caatatctca gatagaaaag gagtttggta agggttcaat aatgagactc ggagaaaaaa
    20221 gattgattga ggttgaagtt atcccaacag gagctatttc tcttgatatt gctcttggag
    20281 ttggaggaat tccaagaggt agagtaacag aaatttttgg acaggaatca agtggaaaga
    20341 caacccttgc tcttgaaatt cttgctcagg cacagaaaca gggaggtact gttgcattta
    20401 ttgatgctga acatgcgctt gaccccaatt atgcaaaagc actcggcgta aatatagagg
    20461 atatgcttgt aagtcaacca gatacaggtg aacaggcact tgaaatagca gaagcactta
    20521 taaggagtaa tgctattgat gttgttgtta ttgattcagt tgcagcactt gttccaagag
    20581 cagaactcga aggagaaatg ggagatagtg ttatgggact tcaggcacga cttatgtctc
    20641 aggcattaag gaaactaacc ggatatataa gtaagtcaaa aacagcagca atttttataa
    20701 atcaggtcag ggaaaaaata ggtgtgtttt ttggtaatcc agagacaacc cctggtggaa
    20761 gggctttaaa gttttatgca tcaattcgta tggaagtaag aagaattgac agtataaaaa
    20821 gagggaatga tatagtaggc agtagaataa aggttaaaat agttaaaaat aaagttgctc
    20881 caccatttaa agaaacagaa cttgatattt attacggtca gggagtaaac aaggaatcat
    20941 ctttaattga ggcagcactg aattatggaa ttttggaaaa gaaaggaagt tatttttcat
    21001 acgaaggaaa aactcttggg cagggtatag ataatataat tgaaaactta aagaaaaatc
    21061 ctgctcttta caaagaaata gagaaaaaaa ttaaggaaaa agtaggactt ataaaaacag
    21121 aaataaaaac agaaaaaaag gagggaaaag atgatgaaag aagttaatgt tgctcttatt
    21181 ggttataaat ttatggggaa agcacattcg tttgcatata aaaatgtatc tctatttttt
    21241 gaccttcctg ttaaggtggt tatgaaagtt atctgtggaa ggacagaaaa ggcagtaagc
    21301 caggcaaagg aaaaatttgg ctggcttgaa tatgaaaccg actggaaaaa ggttgttgaa
    21361 agaaaagata ttgatttgat tgatattact acccctccaa atgctcataa agatatagca
    21421 atagaggcag caaaaaatgg aaaaatagtt ttctgtgaaa aaccacttgc ttcaacttta
    21481 aaagatgcct atgaaatgct tgaagcagtt gaaaaatatg gtgttaagca tgcaatatgc
    21541 tttaattata gaaaacttcc tgctattgga cttgctaaaa aacttataga agaggggaaa
    21601 ataggaaaaa tttatcatat gagagcggtt tatttacagg actggattgt tgaccctgat
    21661 tttccacttg tctggagatt acagaaacag gttgctggaa gtggtgccca tggtgattta
    21721 aatgctcatt taattgatat ggcaagatac cttgtgggtg aatttgatga ggttgttgga
    21781 attaatgaga catttattaa ggaaaggcct gttttaaagg caaaggaaga acttgagaca
    21841 ggacttggtg aatttaaaga aaaggcggag gaaaaaggaa ttgttgatgt tgatgatgct
    21901 actatttttc ttgtaagatt taaaaatggt gcaattggtt cttttgaagc aacaagattt
    21961 gctcctggca ggaaaaatgg tcagagattt gagataaatg gaagtaaggg aagtattgta
    22021 tttaatcttg aacgacttaa cgaacttgaa tattacagta gagatgatga aagtggaact
    22081 cagggtttta gaactattat agcaacagac gcttcccatc cttatgctgc aaactggtgg
    22141 ccagcaggtc atataatcgg ttatggagag gcatttgtaa atatggttgc agatatattc
    22201 aattcaatct ctaagggaga aaatcccagt ccaaatttct atgatggagt taaatgtcag
    22261 gaagtccttg aagcagttga ccagtcaatt agggaaagaa aatggattaa ggtgaaataa
    22321 acctttttgt tctgaaaatc atctaaataa tcaaaaggag gcagaaatga aaaaaaatat
    22381 aatgataggg tttatttttg gattgatttt ttcaggactt gtttttactg gatacagtgc
    22441 aattgaaaag aaaacagaaa gtaaaaagtt ttattcagga ataattacgc ccacatctct
    22501 tgatgaacct cttactttac agagtgcatt tgttcaggtt gcaaaatcag ttaaacctgc
    22561 agttgttcag attacaacag aaaagatagt tacatataga tactgggacc catttggaga
    22621 tctggatgaa tttttcaggt ctccttttga agaatttttt ggtacaccaa ggagaagaca
    22681 gcaaccaaaa acctatcaga aaaaacagga aggacttggt tcaggtttta tagtagatga
    22741 ggatggatac attttaacaa ataaccatgt tattgaagga gttgataaaa tacttgttaa
    22801 acttcttgga gatgaaagaa agtatgaagc aaaagttata ggaactgacc cgaaaacaga
    22861 ccttgcttta ataaagataa atgctggaaa gcctttacct tatgttaaac ttggagattc
    22921 tgataaaatt gaggtaggtg aatgggtaat tgctattgga aatccttttg gacttgaaga
    22981 aacagttaca gttggagtgg taagtgcaaa aggaagaagc ggttttggga taactcaata
    23041 tgaagatttt attcagacag atgcagcaat caatccaggt aattcaggtg gtcctcttgt
    23101 taatataaga ggagaggtaa taggaataaa tacatttata attgctcctt atgttgcaca
    23161 gggtgttggt tttgccatcc caataaatct tgcaaaaaaa gtttttactc aattaaagga
    23221 aaagggaaag gttacaagag gatatcttgg agtgtacctg caagcacttg atgaagaact
    23281 tgcaaaatca tttggtttgt ctgaaataaa aggtgctctt gttgctcagg ttttaaaaga
    23341 tagtccagcg gaaaagggtg gaataaaaga tggagatgta atcattgaat atgatggaac
    23401 tgaggttaaa gatgttaagg atttacagat gaaggttgca aatactcctg ttgggaaaag
    23461 agtttcagtt ggtatatgga gagataagaa aaaaattgtt ttaaatgtta cagttggaga
    23521 aatgcctgct gaagaacccg tcgccgcaga gacagaagaa aaactatgga gaggtatgag
    23581 agtatctgct ttaacagaag aaatgaaatc tcagtttggt atagatgaaa atgaaggtgt
    23641 tgttgttact tatgttgaaa caggttctcc cgcagatgaa agtggaatta aggttggaag
    23701 tgttattaag atgataaatg attataagat ttcaaacatt tcagattata gaaaggtaat
    23761 aaaaaacatt ccgaaaaata cagatgtcag ggttcaaatt aaatttggag atagaacaag
    23821 atttttggta ttgaaaggtg aaaaataatt ttgtaaggat tttatgtgaa gaatgaagaa
    23881 ttaattaaaa aaattttaaa attattgagc aaaaaggact attcagaaga agaaataaga
    23941 gagaaaattc cttcaataac accttctatt ctgaaagaac tgaagaaaaa aaatttgatt
    24001 gatgattatt ctctttcctt aaagataata gaaaatctgc aaaataaagg aaaaggttat
    24061 cattatatat ttaaagaact tgagagaagg aaaataaaag accagattat tgaaaaattg
    24121 aaaaaggaat acgattttga gaaagaagtt gaaatctgta aaaaggtagt ttcaagaatg
    24181 gaagggaaat caatttcctc aattattctg aatttgaaaa gcagaggatt ttcagaagaa
    24241 attattgaga aggttattaa cttttaaatt tgaaagaaat gtcttaaaaa tgatagaata
    24301 aaaaagttat tgactgaatt tttaagttct tgaaagaaaa atagaaatgg aaagtaaaaa
    24361 aattagagaa agttatttaa aattttttga aaaaaaaggt cataaaattg ttaaaagtag
    24421 cagtttaatt cctcttgaag accctacctt actttttaca aatgcaggta tggttcaatt
    24481 caaaaagttt tgggcaacag atataccact tccttataca agggcaacta cctgtcagaa
    24541 atgtttgaga gcgggtggga aggatagcga ccttgaaaaa attggagaaa gtggaaaaca
    24601 tcacactttt tttgaaatgc ttggcaattt ttctttcggt gattacttta aaaaagaggc
    24661 gattgaatgg gcttttgaat ttgttataga tgttttaaaa ataagagaag aaaatttatg
    24721 ggtatcttat tttaaggatg atattgaaac aaaagattac tggaaaaagt ttctacctga
    24781 agataagtta attcctcttg gtgaaaagga taatttctgg ggtcctgcag gagagacggg
    24841 tgcctgtggt ccctgtacag aaatttattt tgattttgga aaagataaag gatgtggtag
    24901 agaagattgt aaacctggat gtgactgtga gagatttctt gaattctgga atcttgtttt
    24961 tcctcaatat gataagcaaa aagatggggc ttttttacct cttaagagaa aaggagttga
    25021 tacaggaatg ggactagaaa ggatttcaag aattttacag ggagttgaga caaattatga
    25081 tacagacctt tttgttccaa ttataaaaaa aattgaagag ataagtggtt tttcatataa
    25141 agaaaataaa agaaatttca ggattatttc agaccacata agaggtattg tttttgcaat
    25201 aagtgatggg atttttccgt caaatgaagg aaggggatat gttttgagaa gaattataag
    25261 aaaagcggtt ttcagtggtc ttgaccttaa tttaaaagag ccattccttt ttaatctttc
    25321 agaagttgtg ataagaaaga tgagtgaaat ttatccagaa ttaatggaga ataaaaaagt
    25381 aatagaaaaa gttatacacg aggaggagga gagatttctt tctttaattt cttcttccag
    25441 aaaaatattt tttgaaattg ttaaagatca gaaggaaatt aaaggagaaa cattatttaa
    25501 actttatgat acttatggaa taccaaaaga ccttatagat gaacttgctt ttcaatatga
    25561 taaagaaata aaatgggatg aatttgatga atatatggta aaacaaagag aactttcaag
    25621 agcaaaatca aaaataggca tgaagaaaaa ggaaatttta atatctgata aaattttaga
    25681 aacagatttt tcaggatacg aaaaaacttc tgatacaggt aaacttgttg ggatttatca
    25741 ggatgaaaat gatttctttt atcttgtttt tgatagaaca gttttttatc ctgaaaaggg
    25801 tggtcagatt ggtgatagag ggattattta caatgataaa tttaagttta aagttattga
    25861 tactcagata gatgaaaagg gacttattta ccatattgga aatgtcttaa atggagggat
    25921 agatgactta aaaattggag atgtatttta tttaaatgtt gatgaagaat tcagaaggag
    25981 tgtatctata aatcatacct caacacattt acttcattac gcattgaggg aaataatagg
    26041 gaaagaggta aggcaggcag gttcttatgt tggagatgat aaattgaggt ttgattttgt
    26101 atgttttaat gatattgatg aggaaataat tgaaaaggtt gagaatttag tatgggagaa
    26161 aatttttgaa aaagcaactg ttaaagttga agaaatgact ctaaatgaag cgattgaaaa
    26221 aggagcaatt gcacttttta ctgaaaaata tggggaaaaa gtaagagttg tttcaacagg
    26281 tgattttcat aaggaagttt gtggtggaac tcatttaaaa aatacaggag atattttttt
    26341 atttcatatc acatcatttt caactattgg gaaaaactta aaaaggattg aagcagtaac
    26401 ctataaaaaa tgtcttgatt atttttctaa atctatagat acatttaaaa atctttcaaa
    26461 tcttttaaaa acagatgaag gaaagattaa agatagaatt gaaaaacttc ttgaggaaat
    26521 aaaagaaaaa gaaagattaa ttgaaaagta tgttaatcta aatattgaaa aaattgtaga
    26581 agaaatttta aagaatccaa aaaaaatctt gaaaaatgga aaggaaataa atttcttttt
    26641 ccgcagtgtt gattctgtag aaaaaaaaga cgatatagca aaaatttctg acaggataac
    26701 agaaaaagtg aaaaatagtt tagtatttct tatttctgaa agtggtgaaa aaaagatttt
    26761 tgtattgaaa acaggcgaag aaattaaaaa tgaaatttct gcaattaaaa ttgtaaaaaa
    26821 tttatctaaa tatataaagg gtggaggaag cgaaaaattt gtaagtggta ttattgaaaa
    26881 aaatataaaa tttgagttta ttgttgatga gataataaaa atactggaaa atgaataaaa
    26941 aaatgaaaaa aaatcagatt ctggaagatg aaagaaaaat tataacaagg attagaaaag
    27001 gagataaaaa attaagagag gaatttatta aaaaaaatca gggacttatt attcacattg
    27061 caaaaaaata tgcattttca aacgaaattc ttccagattt aattgcagaa gggaatatgg
    27121 gtttgataag agcaattgaa aaatttgatt ttagaaaaaa agtaaaattt ggaacctatg
    27181 cttatttctg gattaaaagg tttgttttaa gagcattaat gaaagaattt gaaattttaa
    27241 aaatacctga aagataccat gagtttaaag aaaaaatgga tgaagttaat aatgcataca
    27301 atttaaaatt tggaagagat gccacagatg aagaacttgc caaggaattg aaacttcctt
    27361 tgaatattgt taaaaaattt aaaaaatatt ctgaagattt aacaattatt tcctcggatt
    27421 attataatgg agagaaggat gtagaccttt ttgagttaat agatacaaca ggaaagaaaa
    27481 cagaaaagtt ctgggaaatt ttaagaaata aggatatctt agataaaata tttgagagaa
    27541 taaaaaagaa ggaaaaaaga gcaaatgttg atgtatggtg taaagtttta aaattgcatt
    27601 atggacttga aaatggtata caatatagtt ataaagaaat agcaaaaaaa cttggggtaa
    27661 caagacagag ggtacatcag ataaaaaaaa tatgtttaaa aaaattaaga aaagaatggg
    27721 aggaaatgaa aaatgaagga tttaaagagt ttaattcgga caatccctga ttttcctaaa
    27781 aagggtattc tgttcaggga tattacacca gttcttcaaa atgcagaggc gtttagaaaa
    27841 gtaataatga cttttaaaaa agaaacaccg caagatgtta caaaagttat tgctattgaa
    27901 tcaagaggat ttatttttgg agcatctctg gcttacttac ttggagttgg ttttgttcct
    27961 atcagaaagt caggaaaact tccatggaaa aaagtcaaga tggattatga ccttgaatat
    28021 ggaacagata caattgaaat acaccaggac gctatagaaa aaggagaaaa agttatactt
    28081 gttgatgact tgcttgcaac aggtggaact gctcttgctt cctgtaaact gatagaaaaa
    28141 tgtggtggta ttgttgaaaa aatattattt cttgttgaac ttacagacct taatggcaga
    28201 gaaaaactta aaaactacga tgtcttttcc ataattaagt tttaagattt aagacagagc
    28261 gggactcttc aaataaggcc catttctatc attttttctg caatctgaat agcatttaag
    28321 gcagcgcctt ttcttatatt atcagccacc acccacatat caagagaatt ttctaatgca
    28381 ggattttttc ttattctgcc aacaaagacc tcatcttttc cggaaacaaa cagaggcaca
    28441 gggtacaaac ttttttctgg ctggtcaatt acttctattc cagaagaatt cttaagtatt
    28501 tcccttgctt tttcaacatc aacttctttt tcaaactgtg cggttatact ttcagaatga
    28561 ccgttaaata caggaactct cactgttgtt gctgaaacag gtaaatcaga taaattcata
    28621 attttccttg tttctttaat cattttgact tcttccgtat aataaccctc atattcatca
    28681 cttaaagaac ctatttcagg gataacattt ccaaaaattt gatatggata aacttcattt
    28741 acaattcttt ctccttttac ataactttca acctgcttac ttaattcttc aactgctttt
    28801 tttcctgtac cggaaactgc ctgataggtt gaaacaataa ccctttttaa attaaattca
    28861 aggtgcaatg gataaagtgc aacaaccatt tgaattgttg aacagtttgg atttgctata
    28921 aaaccctgat gtttttttaa agcatcagga ttaacctcag gaacaacaag tggtactcta
    28981 tcatccattc tgaaatcagc accattatct attacgatac aacccatttt aaccgcttcc
    29041 catccaaaaa gtttacttgc acctttttca ccttctgttc ctgcaaaaaa agcaatgtca
    29101 atacctttaa atgattctat ttttgtctct tctattttaa aaatccttcc cgtaatttct
    29161 tcttcccttt ctttcgttgc aaggattttt aaatttttaa acggaaaatt cctttcaatt
    29221 aaaatttcaa ccatcttttt acctaccata ccggcaccaa caacacacac attataattt
    29281 ctcatttctc ctcctcctgt aaatctttca aatgaaaaat attttttgca aaaaggcctg
    29341 ctaaaattgc tttgataata tcaaaaggaa taaatggtaa aactccaata aaaattaaat
    29401 cttttaatcc tttattgaca aaaaatttaa gccagataat tccagtaaag tatataatta
    29461 aagttgcaaa aatcataccg gaaagtttat tctttttttc agaaaaataa cctgcaatat
    29521 atgaagcaaa aataaatccc actaaataac cagtagtcgg tcttaaaatt ccagaagtac
    29581 cgccataaaa ccatccaatt cctgaaattc cacccagaaa ataaaaaatc tgtgataaag
    29641 aaccaaaatt ttttccaagt aaaattccag atagtaaaac tgtaaatgtt tgcatggtta
    29701 aagggacagg agtgaatgga agttttatat aaatctgagc agaaagacct gttaaaattg
    29761 aaaaaataat tgaaagcaaa attttttcag ttaatccaat taatctgtat atctcaaaat
    29821 atttatccca tgtttttatc cagatttttt ttatcataat tttctccttt aaagttcacc
    29881 tattatttcc tttaattttg tttcatctcc cttttttgat ataactgcaa gatatgaatt
    29941 ttctttttta tatatatctt ttgcaatttc ttccacttct tcctttttta ctttttcaac
    30001 ttttttaact atctcattta ttttttccat tttttctttc agtaatttct gttctccaat
    30061 ccatagcata tattcaagat tatcctcaag tcccattaaa aactgtgata tcaaaaacct
    30121 ttttgctctt tctacttcat cttctttaac tcccttttct tttatttcct tcaattcttt
    30181 tacaatttca accatcgctt tttcaagatt ttcaccggat acaccagcag aaatattata
    30241 aattcctgta tctttgtact gtcttataaa acttcttatt tcataagcaa gtccaagatt
    30301 ttcccttatt ctgttgaata atctgctgct catatttcca ccaagaatta catttaaaat
    30361 agataatggg tatttccttt cgtcctggta agaatatgtg aaacctccaa atgaaatatg
    30421 tgtttgttct gtatctttta ttaaaagttt aacttttgga ccattatctt ttttatccca
    30481 tttttcaaat tgattttctt tttttctttc aatcccatca aaaaatttgt caaataactt
    30541 tttcatttct ttaacttcaa attttcctgc aacacttaca acaatgtttg ctcctgaata
    30601 ttttttatct atataatcaa caatatcctc tctttttatt cttgttaatg tttcaggagt
    30661 tcctgctatt ggcataccaa gaggatgttg tccaaaaatc agagtatcaa ataattcatg
    30721 aacatatttt gcaggtatat ccctgtacat atttatctct tcaataatta catttctttc
    30781 tttttcaata tcctcttctt ttaaaagtgg attttttatc atatcagata gaacatcaaa
    30841 acttaaattt aagtatttat caagaatttt aatccagtaa caggttcctt cagcagatgt
    30901 aaaaccatta aaacttcctc caactccttc aatcgcttcc tttatttcat tacaacttct
    30961 attttttgtt cccttaaata aaagatgttc aataaaatgt gaaataccag catattcagg
    31021 gttttcaaat ctacttcctg tttttatgaa tacaccaaca gcaacagaat gaaattctgg
    31081 aaatctttta taaataagtt ttactccatt ttctttttta tattcatacc agtccatttt
    31141 tttctccttg acatattttt tttgagtttt ataataccat aaaataaaga attttaaaag
    31201 ttcaaaaaaa ggaggtaaaa atgagaatag gatttatgat gtcgtatgat aaagaaagag
    31261 ttgagtttgc aaaaaaagta ggatttaaga gttgtgagtt gagggtagga gttaatgatg
    31321 attttttccc tggtaaggat ggatgggaaa caaaggcaaa agaggttgtt gagtattata
    31381 aagcgaatga tataagaatt tcctgtttgg ctggatttta tgttaatcat atggaccctg
    31441 agaaagaaga agattataaa aaacttgtca gaaatgtaat aattcttgct gaaaaaatgg
    31501 gagttcctgt tgttgctggt ttttcaggaa gaattgtggg aaaaccactt gaagaaagta
    31561 ttcagaaatt tgttgagata tggagtgaac atgctaaatt tgcagaagcc catggagtaa
    31621 agattgcttt tgaacactgc cctatgggat atttccatac accatgtaat ggaataaatt
    31681 ttatgtcaac tccaaaaatg tgggaaattg gattttcaga agttaaatca gaagcaattg
    31741 gacttgaatg ggacccgagc catttgatat gtcaatttat agacccagta atcacaataa
    31801 gggattttgg taaaaggatt tatcatgttc atgcaaaaga tgcacatata aactgggata
    31861 tagtgaaaaa atatggtata tggcatcatg gagcaattga acactgtttc cctggacttg
    31921 gagatactga ctggagatta tgcataaaag aacttataag agcgggatat aaaaatgact
    31981 taaacattga gggatggcat gacgctgtat atcatgacag agtagcaaaa ggtaaagagg
    32041 atgaaggttt gattatcgca ttcagacatt taagtcaatt tgttgttcag gattaattta
    32101 aggaatataa tgttttttag aaggacttga catgaagaaa tttttgaggt aatatgtttg
    32161 ttgaacaatt gaacaaagta taaataatgg aaaaagaaaa gatttttaag acaaaattag
    32221 aagtattaaa agaaaaactt ctctatgaaa tttttaaaaa aaatccaggg gaaaaactgc
    32281 cgaccgaaca tgaacttgcg aaaatttatg gagttaacag aagtacagta aataaagttt
    32341 taagtttact tgaaaacgaa ggacttattg agagaaaaac aggtattggt agcataatta
    32401 aagaaaagaa tgaaagaaga ttaaattaca gaattggtgt tattgtagaa agaagttcag
    32461 gacatgttta tgaatattta acaagactta tgataaaaaa aattcaggac agtagattct
    32521 tttcattact tgttgacctt ggagaaccaa cagataaaat tctttcccat cttgatgaaa
    32581 tacttgaaaa taatcctgaa tttgtaatag tggatggaat tggttatttc cctttttggt
    32641 ttttaaaaga gaatatttct aaaataagaa atttgatttt tataaacaga tttgaaactg
    32701 atttaaaact taatgctaca tacattttaa gtgattatga aaaaggtggt tatataggaa
    32761 caaaatatct ctttgaaaaa acaggagaaa agatacttat agtgacgcag agcaatccct
    32821 cttatagacc aaatatagat gaggagaggt tgaaaggtgt taaaagggct tttgaggaat
    32881 atggaattga atttaaagat gaaaatttaa ttgttaataa aaatgagaaa tatttgaaag
    32941 aagcaattag aaaaattttt gaaaaagaaa aagagataag aggtattttt acatttgctg
    33001 ataatgttgg aagacaggtt caggaaatac ttgaagaatt aggaatgtgg cttggagttg
    33061 attatattat ggtcggttac ttcaatacaa gccactcaat tgatttttct ccacaattac
    33121 catcaatatc aataaatgaa aaaggactta ttgaaaaact cggggaagtt ttgaataccg
    33181 ggtttccaaa agaaattttt aaagttgaac ctattcttat tgaaagaaaa ataagagaaa
    33241 gggaggtgat atcatgaaag aaaaagggtt cacgttgata gaacttcttg ttgtaatagc
    33301 aatcatagca attcttgcag caatgcttct acctgctctt gaccaggcaa ggaaaagggc
    33361 tcaaatagca acctgtatga gtaatttaaa acagatttat gttgctctga gtatgtacag
    33421 aaatgattat gataaatatt ttccaactgc ctatgaacca agagcaaact catgttctgc
    33481 atctttaacg ctcttaacag gtcaatatgt ttactataat tctcctacaa aagaaggtcc
    33541 tgattatctg aaaaattata gtttatttat atgtccagca agcagtagaa cacaatcgcc
    33601 aacaggagtt gctgactgga tgaattgttc ttatgcatat gcaaggaatt ttgctgaaaa
    33661 aaatgaaaga gaaccaaaaa aatatgccat tatggctgac ttaaaaggtg actggcagta
    33721 ttatgataca ggaaaacagg caaatatact atgtggttat ttaataagga atcagagtta
    33781 catgccaaat cattcatttg atggagttaa tatccttttc cttgacggtc atacagaatt
    33841 cagaggaaat acaggaacta aaaaagacca gtggggaaac agaatgagtg ttttgaatct
    33901 ggcagatata agtaatattg caacaggaaa tacagaccca tatgctttat tttatccatt
    33961 aggattttaa attaaaggag gtggtattat gaaaagaaat ggcttcacat taattgaatt
    34021 gcttgttgta gttgcgatta tttcaattct tgctgcctta ttacttcctg ctttaaattc
    34081 agcaagacat aaagcacgac ttgcaacatg tatgaataat ttgaagcaaa ttggtatgtg
    34141 tatgaggatg tatattgagg actgggaagg atttattcca ccgaaaaatg acggtgttcc
    34201 aattaactgg ttactcgaat caaatattgg aacagataaa ggaggaccaa gtggaaatag
    34261 taatacacct catcgtcttg cacttttaag atggaaatat gattataatt tgaagaaatg
    34321 ggtttcttct tatgctacaa caaataagct ttttttctgt cctgggattg actataaaag
    34381 gtctaaactt tataattatg gaaatagtta ttggatggca agaaactgtg gatattctta
    34441 tagttggaga ggtgcatctt tctgggatgg tcctgtttta atttcatatc caatatcaat
    34501 taaatttgac cagattttca attataaaag atatcatcca aatggatggc ttgcttgggt
    34561 tgcatgtcca aggagtgatt taattaaaga ccctggagca ccacatcagg atacaggtgt
    34621 taatgttcta tactatgatg gttcagttaa atttttacct cataggaaag aaatagtttc
    34681 caatataagt gtggattaca atactgatgg agatgcatgg cttggacaaa catatcaatt
    34741 ctggtattat gcaattgata gttatcagaa aaaattttaa gaattaaaaa ggaggcagag
    34801 atgaaaaaaa ttttaaaagg aatatttt
//
LOCUS       DAOSAT010000007        32955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_980_length_32955_cov_5.537143, whole genome shotgun sequence.
ACCESSION   DAOSAT010000007 DAOSAT010000000
VERSION     DAOSAT010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 32955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32955
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_980_length_32955_cov_5.537143"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="PLF90_01195"
     rRNA            <1..171
                     /locus_tag="PLF90_01195"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            267..1391
                     /gene="dnaJ"
                     /locus_tag="PLF90_01200"
     CDS             267..1391
                     /gene="dnaJ"
                     /locus_tag="PLF90_01200"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOK55933.1"
                     /translation="MGEKKDYYEVLGVSKDASIDEIKKAYRDLALKYHPDRNPGNKEA
                     EEKFKEITEAYEVLSDPEKRKIYDTYGHAGFGPTGFDWTQDFSRVRMDFSDIFGDIFS
                     DFFGDIFGSDFASKTTQKRKTRGSDLEYRIYITLKEAATGTEKYINVSRFDECSVCNG
                     TGSKTKKGKVTCPTCQGRGQVIKSSGFITIAQTCPKCRGEGEILSDPCPNCRGTGRIR
                     NMHKILVKIPAGIESGMSLRLKGQGDIGLNKGERGDLYVTVFIEKDPLFERKEENIYL
                     EVPITVTQAVLGDEIEVPTLAGKAKLKIPPGTQNGEILRLRNMGMPKLNGHGKGDMFV
                     KIKVLIPKRLSRQQISLFQQLKEIENENDYPEIKNFKEKL"
     gene            1388..1696
                     /gene="cutA"
                     /locus_tag="PLF90_01205"
     CDS             1388..1696
                     /gene="cutA"
                     /locus_tag="PLF90_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOK55934.1"
                     /translation="MKFIQIITTSPDKKNCEKIVKELLEEKVVSCCQIIGPVESHYWW
                     EKRIEKSKEWLIFIKCKKKNYKKVEKKIKEIHPYKVPEIISFEISDLSEEYMDYLSEM
                     "
     gene            1710..2501
                     /locus_tag="PLF90_01210"
     CDS             1710..2501
                     /locus_tag="PLF90_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOK55935.1"
                     /translation="MKFLRFFKDGKEFYGIFKKNRIYAINWEGFTKEFKETGQIFSPE
                     EIKFLPPCNPPNIIALGLNYKEHAEEGWEKVLSEPVIFLKATTSLTGHLSPIILPEQA
                     PDEVDYECELVIVIGKKCKNIEEKEAKDYIFGYTCGNDVSARDCQLKKDKQWARAKSF
                     DTFCPIGPWIETEIEPTNLSIKTILNGKIMQNSNTSKMIFNPYKIVSYLSKQMTLLPG
                     TIILTGTPSGVGFARKPPVFLKEGDIVEVEIEGIGTLKNFVIKEK"
     gene            2498..3589
                     /locus_tag="PLF90_01215"
     CDS             2498..3589
                     /locus_tag="PLF90_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK55936.1"
                     /translation="MKIFNIGIIGFGFIGKVHAYAYENLKYYYPEAPFKAKVSGVCTS
                     REETAKEAKEKYGIEFITTDYKELIENHKIEIIDISSPNIYHFEQLIYAIEKKKHIYC
                     EKPIVATVKEAEELERYLKNFDKIHQVTFHNRFIPATIRTKELIENGFIGKPTIFKIS
                     YYHSGSVEKEKPIGWKQEKGAGVLLDLGSHIIDLIYWFLGEFDEVIGNDLILYPERPT
                     KNGKIVKVEVEDHININVKMKNGSIGIIEASKIATGTQDELKYEIYGTKGSIRFNSEN
                     PNYLNVFSIKEEGFKKIFTGGNYRESNFPGPKFTTGWIRAHIHSIYNFLKCVYENKNA
                     SPSLYDGIYNMKVIDKIKKSIETKRWEKI"
     gene            complement(3594..4127)
                     /locus_tag="PLF90_01220"
     CDS             complement(3594..4127)
                     /locus_tag="PLF90_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HOK55937.1"
                     /translation="MVKNILITGIPGCGKTTLIKEILNCVNVKKFGFYTEEIKEKGRR
                     IGFKIFTLSGKEGILAHISFNTPYKVSKYYVSVENLENIAVCEIERGLLERNCLIVID
                     EIGKMELFSEKFKKVILSAFESQNKILATIMLKSHPFCDIIKMRKDTKVYILDKINLN
                     KIKEEILSNLRIDFKTT"
     gene            complement(4137..5147)
                     /gene="gap"
                     /locus_tag="PLF90_01225"
     CDS             complement(4137..5147)
                     /gene="gap"
                     /locus_tag="PLF90_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOK55938.1"
                     /translation="MVKIGINGFGRIGRLVLRSAIELNYDGFEFVAVNDITDAKTLAH
                     LFKYDSNYGIFNGTVEAKEKSIVINGKEIKVLSEKDPKNLPWKDLGVDIVIESTGLFT
                     EAEKARAHIEGGAKKVVITAPAKGEDVTIVMGVNEDMYDPKKHVILSNASCTTNCLAP
                     IAKVLNDNFGIIKGLMTTIHAYTNDQRILDLPHKDLRRARAAALSIIPTTTGAAKAIG
                     KVIPALNGKMHGIALRVPTPTVSIVDLTVLLEKNTSVDEINSAFKKAAETNLKGILQY
                     CEEQLVSKDFTKNPHSCIFDAPSTYIVDNNFVKVFGWYDNEWAYSCRVVDLVNYVIKK
                     GI"
     gene            5295..6807
                     /locus_tag="PLF90_01230"
                     /pseudo
     CDS             5295..6807
                     /locus_tag="PLF90_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
     gene            7194..8132
                     /locus_tag="PLF90_01235"
     CDS             7194..8132
                     /locus_tag="PLF90_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOK55939.1"
                     /translation="MKLKIFLFVFLFFISIFLPAQNIKIPENVEIIKDVVYGEVDGKK
                     LLLDIVKPEKNEGKMPVIVFVHGGAWRGGDKQGGIYRLIQFAEKGYFCVTINYRLSQE
                     AKFPAQIEDCKCAIRFLRGNSEKYNIDQDKIGVWGPSAGGHLVALLGTTGDIKEFEGN
                     GGWKEYSSRVNAVCDWFGPSDFLTGFEKSNKEGSPYKVISELIGGEIELLKEKAKKAS
                     PVYYVNKDCPPFLIMHGDKDNVVPYVQSVILYNKLKENNVPVTLVKIKDGGHGTGFKK
                     KAMDFVESFFDFYLKGEKEKWQSLRGNNDFIEIPAE"
     gene            8173..9087
                     /locus_tag="PLF90_01240"
     CDS             8173..9087
                     /locus_tag="PLF90_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOK55940.1"
                     /translation="MKIFIIGGTGHIGKNLVKMLEEEKFDLFILTRGVKPLPENNRIK
                     FFKKNYDSNKEEWKNIFDEVKPDVVVDILGTFAPCIYEVCKIYIKHFILCGSIWMFGE
                     PKKVPTPEETQSPCIFSGYAKRYLEMQEIKIQTKKDGVNFTAIMPPNICGPGKIPLDC
                     YGERSIENHKKHKKGEIVFLPEPGQTLIGPCDAEDVAKGFFLSIIQPSNSEDEIFNVG
                     SEYAITVKNFVETYGKIYNVNIPVEWVSWREYSEKINPQPGANTHFKWHMCPDISKIS
                     KKLGYKPRYTPEETMERAVRWMEEQNLI"
     gene            9101..9889
                     /locus_tag="PLF90_01245"
     CDS             9101..9889
                     /locus_tag="PLF90_01245"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOK55941.1"
                     /translation="MKIIIITDLEGVAGVVDFETQALSGSKYYETAKELATEEVNATC
                     EACIEKDVDEILVIDGHGPGGIIPEKIHKEVKLLHGRPVPTFWELDKKWDGLILLGYH
                     SMNGTEKGNLNHTYTLEIVNMWLNGEKIGEIGMKIYLAGWFGIPTILISGDEAAVKEA
                     EKYVSNIEKCIVKWGITKTSAISLSPLKAREIIKDKTKRALERIKEIKPVKLEGKCEI
                     VIEYLSTADAFNRGEKSDAELIEPTKVRIKGENFIDVWKKFYRG"
     gene            10077..12137
                     /locus_tag="PLF90_01250"
     CDS             10077..12137
                     /locus_tag="PLF90_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOK55942.1"
                     /translation="MLKKFERIFKIVIIFSCLYLLNLFSSDSVSVIFSVPIVKNGIEV
                     KVGPNSGAESIKNYAGKTGLETNILKEAGVIYCDIDDKFIYDGINKVLIKVEYWDKKV
                     NSDEGFYIAYDSVGEPRVYLPKIYFEGTNTWKKIEYYVDNAKFANRQNGADLRVFSPE
                     TDICIRNITIEKVPEKVPIKNVEIVPSSIVLIEGKSFRPSLIINPYYATDKIFKWSSD
                     NPKVATVNSDGVIVAVTGGNTIVRAVSRDGKFKAECNVKVISVKGKGILAERVNDFLD
                     SIGINTSISTRGEIFEKTIECIKYTGIRWIRGMASLDEMKKLNKETGVKFTLPLIQDI
                     NKLIKVGRELAKDGILLGFEGPNEPNNWGFTYRGEKGGGHGANTWVPIAKFQRDSYGF
                     IKSDPELKRYPVWNLSEGGAEVDNVGLQFLTIPEGVDTIMSAGTQYADYANCHNYACH
                     PTLPALIDNQAWYAADPVIKAVPEYEERGGHFDGLFKNYGETWLKKYKGYKEEELKVL
                     PRVTTETGITIDESRGITEEIQGLLYLHIYLSQYKRGWRYTSIYILRDRSDETGNQTF
                     GFYRPDYSPRKAAHYLHNLTSILKDEGKEFIPGGMGYEIEGQNEYVHDLLLQKSDGRF
                     YLIIWNERFGSKKEDEVVVKLSKRYKEIKIYDVVKGIEPVEKLKETDTIKLKLKNSPL
                     IVEI"
     gene            12162..12848
                     /locus_tag="PLF90_01255"
     CDS             12162..12848
                     /locus_tag="PLF90_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55943.1"
                     /translation="MKKLILVLTIVFGLASLCSAEVIFKTDFSKGDFADLGWIVKGDW
                     DIFKYGVKNDPGPVARYPTNKPDGGTLTKKFEEVKDPQKLILSFDYGWGWGAEDQGAD
                     SVSIMLLDSNGDGYIFSIGRWKAKWCFQWGIVKNGVPPKEKKWAPEDMDFTLKSIRDP
                     NFEKLGHVIITREKGGNWIITGENWNKGKGGTIKFTDNTVSSFSQVVLYGTKNFDEQV
                     FNNIMLDVTK"
     gene            12908..14188
                     /locus_tag="PLF90_01260"
     CDS             12908..14188
                     /locus_tag="PLF90_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOK55944.1"
                     /translation="MIKANVMGLLISLISFTGCTQKFLNKESNKENETKVLPAKVVPQ
                     RVPQGSSFKVYFNWESDGPLSKPYKIFVHFLDDNDKIVFQGDHEPPLMHTNLPQWKGK
                     ISYEMKFKVPENLADGSYRIIVGLYDKEGRLKLKAGKGVIEETDLRYRVGILNVDSKA
                     KPVPPDTAGRKTLDLSNYELVFNEDFENHLDVSPWGPGTRWIAHTPWHGDFGDAIFMD
                     PGVYPEFPFKVENGILIIEARKDKKFAEKDPWKREWASGLLCSNDPKGNGFSLQYGYF
                     EMRAKLPGGQGVWPAFWLVSSYDRTDKNAGKDGAIEIDIIEYYGVPDAYSSVIHIWEP
                     KPHRGVGTRVTTRKNEITEDFHNYGCMVTPEWIIMYFDGVEVWRVKTPPEHNKPLMIL
                     LNFALGSGWPIDKVPNSSFMYVDWVRAYAPKKKK"
     gene            14185..15222
                     /locus_tag="PLF90_01265"
     CDS             14185..15222
                     /locus_tag="PLF90_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK55945.1"
                     /translation="MTKKERVKRTILHQEVDIVPFKIDFTIPAWEKLISFLGREPSID
                     EIGNHIVEIEPVTSDAWIEIKPNFWKDEWGIIWNRTVDPDIGNPIPVLKEPSLKNYKF
                     PDPERKERYEKFPEFIEKNRDKFILANIGFSLFERAWTLRGMENLLVDMIENPDFVHE
                     LIEAITDFNLRIIKNVSKYEIDGVRFGDDWGQQRGLIMGIKYWREFIKPYLKIMYDEV
                     HKNGLFVFIHSCGDIKEIIPELIEIGVDVFNPFQPEVVDIYEMKEKYGNKLTFYGGIS
                     IQKLLPFGTPAEIKKEVNKILEIIGKGGGYIASPSHSIPKDVPVENIVALIETLNNQK
                     EKNKMVEINVL"
     gene            15301..16446
                     /locus_tag="PLF90_01270"
     CDS             15301..16446
                     /locus_tag="PLF90_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK55946.1"
                     /translation="MERNNIPFYKQIKEEILKKIEKGEYKVGDKIPSISELSKIYNVS
                     NIVVRQALGELVKEGYLEGIPGKGTFVKEKREEIKRDEKLVGLIFTEMVGNPFFAEIF
                     TGIESVLSSYGYHMVISVSYNIVEREKRFLKEYMERGVRGIIITPTEVNRTVIDNEIF
                     SELQRQNIPVIFVDRKIEGIDFDYISSDNVEGGYIATKYLISLGHKRIGIILGINANT
                     VRDRLEGYKKALQEYDIIFDPLLVRRSFAELQYEEAGYNNTMDLLHLKEPPTAIFACN
                     EAIAIGIYRACFELGLNIPDDLSVIGYDNLPFTSSLNPPLTTINQKKREMGEEAARIL
                     ISRINGDKSGPKKIIFKTELIERNSCKQLKEEEQNEKVRVKKKENSK"
     gene            16463..17296
                     /locus_tag="PLF90_01275"
     CDS             16463..17296
                     /locus_tag="PLF90_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOK55947.1"
                     /translation="MKKKGFTLIELLVVVAIIAILAAMLMPALSKAREKARQAVCMSN
                     LKEIALAFHMYAQDWDDYLPPYGDSYTGDWWTVWPNVQLWAHKIAKYTGTKTFQTYNM
                     PKSKQGVWRCPSVLDSKIGWGGTYGYGVLTGSISGSNIHYGFYWSSNRIFIKLSRIPN
                     KYHAKLMLVADACCFPPHGNTSNRDSGVPIYWATVKQLSQTPETTAASTIWCPICTYW
                     NDNNNPTRYISNRHNGGGNVAFLDGHVEWMSLFDAVRIRNDNPYGCWRAGYSDLKMPR
                     Q"
     gene            17397..21731
                     /locus_tag="PLF90_01280"
     CDS             17397..21731
                     /locus_tag="PLF90_01280"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOK55948.1"
                     /translation="MKKWLIIFIFCTFLFSAEKIDLFEKDKELYGWKIGLGLEFKGAK
                     GQLIIDDNVKYNGKPSLKLTGDFTEGGKYVEVSKNLDNLDIESFSFYVKYPKDVLTFR
                     IIDSSGQCHQIRIKIKKTDDWQLVEFPLKKFFEKMGTSDAVSGIMGYEKWSGKNDGRW
                     YGPAKFISILLSPDKNTGNMVTMWFNEFTAVVSEKKEKKEIIKTIPLDDLLKLGQKDW
                     DLFLGTEFKGAKGNLDIVRDGEEYCLKLAADFTEGGAYIDTGKSLGFLNVDAIKSISL
                     KLKSDNVKSFGIRIIDSSGQVLQKKGFKFTPDNKWHDVVINIDDFNGAEHWSGANDGK
                     VHQPVKNIRIVLTTYSSEDKKPVLFIKDIKAGILFQGEVSGTVLEENFEDVVNISENW
                     EIKGNINVIEGDSFSGKKSLVLERSEKNVNVETSALSKIFNVKPGILRIQGVGKSELY
                     SPDNSYCGKVSVEFLDSNKKVIERQEVIIFTGKTNWQTFSKEIEINNNANSARVKIDL
                     QKTYGKLNIDDLKISYISPSSSIVKKVQRIEISSERLGNLFYPEDEVSLNINVKTTLP
                     LSDSEKTLKCEIKDYWGANQTNSLQLKLDRFEIDKGVLIYSTKLKIKEKLEIGKYYEI
                     HITVPQEGEPFKEFSTFAILPEAITKKYKPEEIPFTQRNWDNRIREYFLLSDRLGIRI
                     CGIWSGFDFKPPYNSHAPGLELVNELGMKWVAGTPGAGVEHKRGNWEQFTEEVLKEGM
                     KKFVEKYKNNGLYYICLGNEPPTDYENAVRNTKAYKAMYEGVKEVAPELIVLGTSVGP
                     AENYFKAGFQKYLDMYDFHTYGDYREIPRIFKKYKELFSKYGGEKPICSTELGINSQG
                     MTRYDVAKGLIKSITLFFACGGAHCSWFDLLYPDPQGKAETSAAQAHNVFYCKYNLYA
                     PKLDAIAYYNIVNGICVKKFVESKVYPDGTEVYLFRDKEGNTLLVLWNDKSNSEFFFP
                     LPEVDDVEVIRIDGSRRKLNAGKNGINLNLIQEEPVLILYRQNGKNIPEAFTQPKVRI
                     ISKPDYIIEGDSFKIVFEGENISVDEMEISCPSEFSIRKIQDGKKRVIIEVSIPENFI
                     AREGRFRISMYKEGIDFFLNLPVVSKIEGIILPYPSCLKNGPGVRLILKNNGEKTQKV
                     NWVVSIEKEFPMKNGTFNLLEGQIPNAYFKDMTNGTEELKSKEGKEILLSMEKVDPLT
                     IYQIKCVVKDEFGRGFEFKRYISGFLGAPYRKEEIKIDGDLSESDWNSAPTAFLDKEQ
                     QVYYLKKLETEMWKGIEDLSGKLKFLWDEKYLYIGVEVIDDVHSTPYADGSIWNQDGL
                     QFLFDPKRGEKEKKGKYDISIASGQKGLQCWSHLTADPAKSPTGEIKDVKIGFKRIDN
                     KTIYEIAIPWDKLSPFYPFPGADLGVAMIINEDDGKGRVSFIGWFGCVHSKQMDMVGD
                     LILLEKEK"
     gene            21728..23635
                     /locus_tag="PLF90_01285"
     CDS             21728..23635
                     /locus_tag="PLF90_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55949.1"
                     /translation="MTKRAFILGVIFSIFVGVGEIIWVAWTSGSPMTNDHSAGASIFF
                     LFILCLFVNPVLKKIRKNSALSSSELTLIFIMMLVACAIPSWGLVMNLIYLIAGVHYF
                     ATPENNWFEIIIPKIPKYLTPNDQQAIKFFYEGLPAGVSIPWNAWVQPLIAWTLFILV
                     LSFMMISISVIFRKQWEENEALLYPLMILPSEMIKEGKGIISPFFKDRLMWLGFSISF
                     ILNSLIVLRFYFPLVPKIVLGKWIGFGPGGALGWLSIGLRFEILGIAFLLSSDISLSI
                     WLFTLFYFVEEVIFNRIGLNIGSPDAYSLAVPIAYQMEGAFLVFILFSLWISRSHLKK
                     VFNRIFKNDTSIDDSGEALTYKQAFIGLILGFLFTVLWAKMTGFNLLSSVYFSVVLIL
                     TFIGITKIVVQTGTAYCRPPINPGVFTLHSFGSSRLGSGSAVSCAFTFPYAGDIRTSV
                     MTTSSNGLKIAKNFNLRGRFLFWAIFTGVLVSFISSVITVIVISYKIGGINCWGWQPV
                     GMPPYTFNWAKEMILHYQNVDIRRIGFLLLGGFLMFLLYFLRIRFVNFPVHPIGMAFT
                     YNVPTQWVWFSFFLGWLFKSMILKYGGVSVYRKLLPFFFGLIIGAFFSAGLWNIINFF
                     THQQGIHLTVT"
     gene            23649..25247
                     /locus_tag="PLF90_01290"
     CDS             23649..25247
                     /locus_tag="PLF90_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016161 - beta-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 14 glycosylhydrolase"
                     /protein_id="HOK55950.1"
                     /translation="MKRVTIGAPWHVLYYYDRKYHNYLRKIKATGIETYVKWSDIEKE
                     PGQIDFSNFDRQLEIMKKYGVKWQPFLICGPWYSLPYWFHKSSDCYYFKCLEHSIESG
                     IQSIWNPYFKKYVVRFLNLFYQNYKSKMDLIDSLLLGISGDYGEAIYPVVGNWDGMYH
                     THKGFWCGDDFAIIDFRDYLKEKFEKIENLNKRWNTSYRSFDEIKPFLKENSPSRPAM
                     IDMVYWYRTSMIKWVEFWVKEARKLWKEKDIYLVMGGDSSAKEGQHYFKAAEICSKYN
                     VGIRDTNARDNFPYLHTYQAKTSVATNFYGNYCSYESSSGSNEKLIVARIFTFIITNA
                     REFHEYSFHSNEKVINNFKKYRKLMEINFKRKVDIAVFYPEPYVNWIHEYAISWEIKN
                     LPWGLPPELHNSFYNLRYYFDFDIVDDSLIRNGILEKYRVLIIPSFSIIEDDIKELIE
                     NRIKNKEKIIVFGKETPEDVYGNKINFGEENHIVSLRKLLKILKNMGINVKERKDGIF
                     EVFDEDGRIICYDEIKNKIFWKRR"
     gene            25253..26539
                     /locus_tag="PLF90_01295"
     CDS             25253..26539
                     /locus_tag="PLF90_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOK55951.1"
                     /translation="MKESRKVVDFLNSLGANSAVSRRDEILSKTIECVKYLGIRWIRS
                     GYESNVPVEDLLELHRQTGVVFSYGLLSGGNDINRLLEGGRALARNRALLAFEGPNEP
                     NNWAVKYRGKIGGRDYSWIPVAELQRDLYMSVKNDPELKRYPVWNLSEGGAEVDNVGL
                     QFLTIPEGVDTIMSAGTQYADYANCHNYACHPTLPALIDNQAWYAADPVIKAVPEYEE
                     RGGHFDGLFKNYGETWLKKYKGYKEEELKVLPRVTTETGITIDESRGITEEIQGLLYL
                     HIYLSQYKRGWRYTSIYILRDRSDETGNQTFGFYRPDYSPRKAAHYLHNLTSILKDEG
                     KEFIPGGMGYEIEGQNEYVHDLLLQKSDGRFYLIIWNERFGSKKEDEVVVKLSKRYKE
                     IKIYDVVKGIEPVEKLKETDTIKLKLKNSPLIIEIF"
     gene            complement(26536..27561)
                     /locus_tag="PLF90_01300"
     CDS             complement(26536..27561)
                     /locus_tag="PLF90_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOK55952.1"
                     /translation="MIYKTIGKSDIKVSAMAIGAWQIGGQWGNYDKKEVEKIIDSAIE
                     NGVNFIDTAEAYGESESVLGEILKGKRDKFVIATKIGGNHFDYKTVKEHLTGSLKRLK
                     TDFVDLYQVHWPKMKHLWHKEDMNEKDYYDIADSMTKLQKEGLIRYAGVSNFRKKHLL
                     NFPEEIFSSVIVSNQVPYSLLWRCYDIDGTTNFCIKNKIDFLAYSPLAEGLLTGRFKD
                     RSEIVENVRKANVLFNEPVYSKALKVVEEIEKIAKELDMTIAQISINWCISKNYIASA
                     LVGMRKAKHFEENIKAFGKNLPEEIIEKLDNISMDFQKNYLVEGLELWIGNCLKENLE
                     KIGIKRD"
     gene            complement(27573..28952)
                     /gene="mnmE"
                     /locus_tag="PLF90_01305"
     CDS             complement(27573..28952)
                     /gene="mnmE"
                     /locus_tag="PLF90_01305"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOK55953.1"
                     /translation="MEKDTICAISTPLGISGIGIVRLSGNETYRIIEEIFVPGKAPLK
                     KKFKIEKIPSHTVHYGHIVDNGKIIDEVIVVIMKSPKSYTREDMAEIGCHGGLIPLKE
                     VLNLCIKKGARLAQPGEFTKRAFLNGRIDLTQAESILDIIYSKTETGLEIGIKKLKGS
                     LSNFISEIREKIFNLLVNIEAKINFPEEEDVEEYRLNIEKEIKEIYQEIEKFLLKCNK
                     GKLIQTGVKVAIIGSPNVGKSSLLNALLKEERAIVTEIPGTTRDVITEIVNIKGIPIN
                     IIDTAGIRKAKNKIEKMGVEKSIEWMKKAEINLLMIDGSRKLSEYDYQLLEHIKNKPY
                     LIIINKIDLPQKVEMDKILREFDRDRIVKVSVKNNTGIEEIENKLYQLINEGYGEIKG
                     DEIYLNLRQERKINTVNQYLEEALKETYPEIIAENLKSCINQIDELMGNKISDKILDE
                     IFSNFCIGK"
     gene            complement(28979..29344)
                     /locus_tag="PLF90_01310"
     CDS             complement(28979..29344)
                     /locus_tag="PLF90_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55954.1"
                     /translation="MKKILFLMKEKNLLEKYLFLMKNYEYETTGFLLHDKKLMEKIDK
                     LENVNIVIIEVDECGIEIKKVVKKLKEKFKDCKFLILIPEINDKIQNFIIDFSIDSYL
                     VFPVLPFQLLRSIFLLNNL"
     gene            29444..29779
                     /locus_tag="PLF90_01315"
     CDS             29444..29779
                     /locus_tag="PLF90_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOK55955.1"
                     /translation="MEDFYKKIGRKIREVRKSKKITQEELAYRIGVTPNFIGLIERGK
                     KRPSIETLRKISDALEVPVSYFFDKITYKLPEEDIITKKISAILKDTTEEEKKGIYKF
                     LKSIVRKKK"
     gene            29847..30410
                     /locus_tag="PLF90_01320"
     CDS             29847..30410
                     /locus_tag="PLF90_01320"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOK55956.1"
                     /translation="MGEIKKIHPVKIFCGLIYKEEEILEKTKIKLAEKWGKIDIEEGP
                     FNFDFTDYYEEEMGINLKRKFVSFENLYIPEDVYEWKIFTNEVEKEFSEENKRKINID
                     PGYIDSSKVILLSTKDYYHRIYIGKGIYAELTLYYSKGKYNFLNWTYPDYRTENYISF
                     FLKMKQKYTKQVREFLKNEKNVGENTC"
     gene            30379..31275
                     /locus_tag="PLF90_01325"
     CDS             30379..31275
                     /locus_tag="PLF90_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOK55957.1"
                     /translation="MKKMWVRILVDFFIIVFILSLITGIRAVMPPRMGIFITPKDLGL
                     PYENIILQTEDGKRLKGWFIYSEKAKCVIICLHGYPANKSDILPVVEFLYPEFSLLLF
                     DFRAHGESEGKVCYFGLKEFLDVKSAIVFIRNNEKIKDLPIGIWGYSFGGAIGIISAS
                     KYNDIKCIVSDSAFANFPDMVKSYYKNLGPLKYIFSSFSIFLGRYVFRSDFKLNSPEN
                     FIGSVKCPILIIHSRNDEFVPFTHAKKLYEKAKSEKEMYVVEGSHTGLDRAYTNEYRE
                     KVKSFFEKNLISEKIEKRRHNE"
     gene            31268..32185
                     /locus_tag="PLF90_01330"
     CDS             31268..32185
                     /locus_tag="PLF90_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOK55958.1"
                     /translation="MNEIFKRHPENPILTPEKWKYPCNAVFNPGVIEFEGYVYLLCRV
                     ETFDGYSHFTLVKSKNGVNNWEIPEKPTLVPDERYDEEKWGIEDPRIVYLKEIEKYAI
                     TYVSFSPGGPLISLILTDDFIKFERKGPLVPPEDKDGSLFPRKIKDKFALIHRPIIRG
                     EGHIWISYSPDLIHWGNHKILLPVRAGMWDCHRIGLGCPPIETDEGWLIIYHGIRYTA
                     SIPIYRVGLALLDKESPEKVISRTKRWVFAPEKEYERIGNAMNVVFPTGYIIKDKEIF
                     IYYGCTDKCISLAFASISDILTILLKEKE"
     gene            complement(32199..>32955)
                     /locus_tag="PLF90_01335"
     CDS             complement(32199..>32955)
                     /locus_tag="PLF90_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOK55959.1"
                     /translation="AGTFLVPTMFIGMLNLEVFEKFHLPKLRWAAVFGAPYSPDVIKK
                     FNKVCPQAKLLSGYGLTETAAPNVLPPLEDVRFGSVGKPVPWIEVKIVDEDGVEVPKG
                     EVGEIIMKGWPVTPGYYNQPDLTAQVIRDGWLYTGDLGKFDNDGYLYIVGRKKDVIIV
                     GGLNVYATEVEGVIYKHPKVKEVAVVGVPDKIRGEAIKAVIVPKEGEELTESEIKNYC
                     REHLATYKVPSIIEFRNELPKTGSGKIKKELLR"
BASE COUNT        11998 a         4254 c         6080 g        10623 t
ORIGIN      
        1 atacgttctc gggccttgta cacaccgccc gtcacgccaa ctgagtcggg ggtacttgaa
       61 attccttatc ccaacccgca agggagggag ggaatgagag tacactcggc aaggagggcg
      121 aagtcgtaac aaggcagccg tacgggaacg tgcggctgga tcacctcctt tctaaggagt
      181 tgaaaaaaat ccggataccc caatttctta cttaaaaagc aagggtttcc cttgcttttt
      241 ttatttatat gactaaaata taaaatatgg gagaaaaaaa agattattat gaagttcttg
      301 gtgtttcaaa agacgcttca atagatgaaa taaagaaagc atatagagac cttgctttaa
      361 aatatcatcc agacagaaat cccggtaata aagaagcaga agaaaaattt aaagaaatta
      421 cagaggcata tgaagtttta tctgatcctg aaaaaagaaa aatatatgat acatatgggc
      481 atgctggttt tggaccaact ggatttgact ggacacagga tttttcaagg gtaaggatgg
      541 atttttcaga tatttttggt gatatattca gtgatttttt tggtgatatt tttggttcag
      601 attttgcatc aaaaacaact caaaaaagaa aaacaagagg ttctgacctt gaatatagaa
      661 tttatataac attaaaagaa gcagcaacag gtacagaaaa atatataaat gtttcaagat
      721 ttgatgagtg ttcggtttgc aatggtactg gaagtaagac aaaaaaaggg aaagtcacat
      781 gtcctacctg tcagggaagg ggtcaagtta ttaaaagttc tggatttata acaattgcac
      841 aaacatgtcc gaaatgtaga ggggagggag aaattctgtc agacccatgt ccaaattgta
      901 gaggaacagg aagaataaga aatatgcata aaatacttgt taaaattcca gcaggtattg
      961 aatcagggat gagtttaaga ttgaaaggac agggagatat tggtttaaat aaaggcgaaa
     1021 ggggtgactt atatgttaca gtttttattg aaaaggatcc tctttttgaa agaaaagaag
     1081 aaaatatata tttagaagtt ccgattacag ttactcaggc agttttagga gatgaaattg
     1141 aagtacctac tttagcaggg aaagcaaaat tgaaaattcc tccgggtaca cagaatggag
     1201 aaattttaag attgagaaat atgggtatgc ctaaattaaa tgggcacggt aaaggagata
     1261 tgtttgttaa aattaaggtt ttgataccaa aaagattatc aaggcaacag atttctttat
     1321 ttcaacaact caaagagatt gaaaatgaaa atgattatcc agaaataaaa aattttaaag
     1381 aaaaattatg aaatttattc aaataattac aacctctccg gataaaaaaa attgtgagaa
     1441 aattgttaaa gagttacttg aagaaaaagt tgtttcctgc tgtcagatta taggtccagt
     1501 tgaaagtcat tactggtggg aaaaaagaat agaaaaaagc aaagaatggc tgatttttat
     1561 taaatgcaag aagaaaaatt ataaaaaggt tgagaaaaaa ataaaagaaa ttcatccgta
     1621 taaagttcct gaaataatat cttttgaaat ttctgatttg agtgaagaat atatggatta
     1681 tttatctgaa atgtaaaaag ggagaaaata tgaaattctt gaggtttttt aaagatggga
     1741 aagaatttta tggtattttt aaaaaaaaca gaatttatgc tataaactgg gagggattta
     1801 ctaaagaatt taaagaaacc ggtcaaattt tttcacctga agaaattaaa tttttaccac
     1861 catgcaatcc tcccaatatt attgcacttg gattaaatta taaagaacac gcagaagaag
     1921 gatgggaaaa ggtactttca gaaccggtca tatttttgaa agcaactact tctttaactg
     1981 gacatctatc tccgataatt ttaccagaac aagcgcctga tgaggttgat tatgaatgcg
     2041 aacttgttat tgttattggt aaaaaatgta aaaatataga agagaaagaa gcaaaagatt
     2101 atatattcgg gtacacctgt ggaaatgatg taagtgcaag ggactgtcag ttaaaaaaag
     2161 ataaacagtg ggcaagagca aagtcatttg atactttctg cccgattggt ccatggatag
     2221 aaacagaaat tgagccaaca aatttaagta taaaaacgat tttaaatgga aaaataatgc
     2281 agaattcaaa tacttcaaaa atgattttca atccttataa aattgtgagt tatctatcaa
     2341 aacagatgac acttttacca ggaacaatta ttttaacagg aactccttca ggagtgggat
     2401 ttgcaaggaa accgcctgta tttttaaaag aaggagatat tgttgaagtt gaaatagaag
     2461 gaattggaac tttaaaaaat tttgttatca aagaaaaatg aaaatattta atattggaat
     2521 tatcgggttt ggttttattg gcaaagtaca cgcttatgca tatgaaaact taaagtatta
     2581 ttatccggaa gcgcctttca aagcaaaagt ttccggtgtc tgtacatcaa gggaagagac
     2641 agcaaaagaa gcaaaagaaa aatatggtat tgaatttata acaactgatt acaaagaatt
     2701 gattgaaaac cacaagattg aaattattga tatatcaagc ccaaacattt atcattttga
     2761 gcaacttatt tatgcaattg aaaagaaaaa acatatttat tgtgaaaaac ctattgtagc
     2821 aactgtaaaa gaagcagaag aacttgagag atatcttaaa aattttgata aaatccatca
     2881 ggtaacattc cataatagat ttattcctgc aacaataagg acaaaggaat tgatagagaa
     2941 tggatttatt gggaaaccaa ctatttttaa gatttcatat tatcattcag gaagtgttga
     3001 gaaagaaaag ccaattggat ggaaacagga aaaaggtgct ggtgtattac ttgatttagg
     3061 aagccatatt attgatttaa tttattggtt tttaggtgaa tttgatgagg tgattggaaa
     3121 tgatttgatt ttatatcctg aaagaccaac aaaaaatggg aaaatagtaa aagttgaagt
     3181 tgaggatcac ataaatataa atgtaaagat gaaaaatggt tcaattggaa taattgaggc
     3241 atcaaagata gcaacaggga cacaggatga acttaagtat gaaatatatg gaacaaaagg
     3301 tagtataaga tttaattctg aaaatccaaa ttatttaaat gtcttttcta ttaaagaaga
     3361 gggctttaaa aaaatattta ctggtgggaa ttatagggaa agcaatttcc cagggcctaa
     3421 atttacgact ggatggataa gggcacatat ccattcaatt tataattttt taaagtgtgt
     3481 atatgaaaat aaaaatgcaa gtccttcact ttatgatgga atttacaata tgaaagtcat
     3541 tgataaaata aaaaagtcaa ttgaaacaaa aagatgggaa aaaatataaa attttaagtt
     3601 gttttaaaat caattcttaa atttgataaa atctcctctt taattttatt gagatttatt
     3661 ttatcaagaa tataaacttt tgtatccttt ctcatcttaa taatatcaca gaatggatgt
     3721 gattttaaca ttatagttgc aagaatttta ttctgacttt caaaagcaga gagtataact
     3781 tttttaaatt tttctgaaaa caattccatt ttgccaattt catcaattac aatcaaacaa
     3841 tttctttcta aaagtcctct ttcaatctca caaacagcaa tattttctaa attttcaaca
     3901 gaaacataat attttgaaac tttataagga gtgttaaaac ttatatgtgc taaaatacct
     3961 tcttttcctg ataaagtaaa aattttaaac ccaattcttc tgcctttttc cttaatttcc
     4021 tctgtataga atccaaattt tttaacattt acacaattta aaatctcttt aattaaagtt
     4081 gtttttccgc acccgggtat acctgtaatc aaaatgtttt ttaccaaatt acaagtttaa
     4141 atcccttttt taattacata atttacaagg tcaacaaccc tgcaactata agcccattca
     4201 ttatcatacc atccaaaaac ttttacaaaa ttattatcaa ctatatatgt tgaaggagca
     4261 tcaaatatac aggaatgtgg attttttgta aagtcctttg aaacaagttg ttcttcacag
     4321 tactgtaaaa tcccctttaa atttgtttct gctgctttct tgaatgcaga atttatttca
     4381 tcaacagagg tatttttttc aagaagaaca gttaaatcca caatggaaac agttggagtt
     4441 ggtactctta aagcaattcc atgcatcttc ccatttaaag caggaattac ttttccaatc
     4501 gcctttgccg ctcctgttgt tgtaggtata attgatagtg cagctgctct tgctctcctt
     4561 aaatccttat gtggaaggtc aagaattctc tggtcatttg tataagcatg tattgttgtc
     4621 ataagtcctt taattattcc aaaattgtca tttaaaactt ttgcaattgg agcaagacag
     4681 ttagttgtac atgaagcatt tgaaagaatt acatgttttt tagggtcgta catatcctca
     4741 ttaacaccca taactattgt aacatcctct cccttggcag gtgcagtaat aacaactttt
     4801 tttgctccac cttcaatatg tgcacgtgct ttttccgctt ctgtaaaaag gcctgttgac
     4861 tcaattacta tatcaacacc taaatccttc catggtaaat tttttgggtc cttttcactt
     4921 aaaaccttta tttcctttcc atttataact atactttttt cttttgcttc aacagttcca
     4981 ttaaatattc cataatttga gtcatatttg aaaagatgag caagtgtttt tgcatcagtt
     5041 atatcattaa cagcaacaaa ttcaaatccg tcataattta attcaatagc acttcttaaa
     5101 accaatctac caattcttcc aaatccatta attccaattt tcaccatttt ttcctccttt
     5161 tttaattttt tattatacca gatttttttt atttaagtca ataaaatttc aataatgtaa
     5221 tattttgaca aaaaatcact ttacatattt tcagttcaat gatattattt ttctaatgaa
     5281 aaattgaaaa aactatgata agaaaaattg gttttgataa cgaaaaatat cttgaggaac
     5341 agagtaaagc aattcttgaa aggattaatc tttttaaagg taaattgtat ctggagtttg
     5401 gaggaaaact tctatatgat tatcatgcag caagggttct tcccggttat gattgtaaca
     5461 taaagataaa acttctgcag gaattgaaag ataaaattga tattattctc tgtatatatt
     5521 caggagatat agcaaaaggc cgtattagag gagatttttg gaattactta tgatgcagat
     5581 gcactaaaac ttattgatgg gttaagagaa tatggtcttg atattagagc ggtagttata
     5641 acaaggtttg aaaatcagcc caatgttgtt gcctttaaaa ataaattaga atatagaggt
     5701 ataaaagtat atcttcatac agcaacaaaa ggttatccaa ctgatgtaaa tactattgtt
     5761 agtgaagaag gttatggaag aaattcatat attgaaacag aaaagccaat tgtagtggtg
     5821 acaggacctg gaagtggaaa actcgcaacc tgtttatctc aaccttatca tgattacaaa
     5881 agaggaataa aatctggcta tgcaaaattt gaaacattcc ctgtctggaa tttaccacta
     5941 aaacatccag taaatattgc ttatgaagca gcaactgctg acctcggtaa tgttaatatg
     6001 attgaccctt atcacctgaa tgcttataag gaaagtgctg taaattataa tagagatata
     6061 gaactttttc ctgtattgga gagaatatta aagaaaatta tgggaaataa tcttgtatac
     6121 cgttctccca cagatatggg agtaaaccgt attggttttg ctatagtgga tgatgaagtg
     6181 gtaagagaag cagcaaaaca ggaaataata agaaggtatt tcagatataa ctatgaattt
     6241 gttcttggga ttgaatcaca taaaactctt gaaagagtta tacttttaat ggaagaactc
     6301 aatttaaaac ctgaagacag gtctgttgtt aaaccagcaa gaatgtcggc agaaagagca
     6361 aaggagaaga taggtaatta ttccagtgta tattgtggtg ctgctattga agtaccggat
     6421 gggacaataa ttacaggaca taattcagaa ctcatgactg ctgcttcaag tatgattctt
     6481 aatgctataa aatatatggc agaaataccg gataaaattc atctcctttc aacaaatatc
     6541 atagagcata ttaaaaaact taaaggagaa tttcttggac ttaaaaatga aattttagac
     6601 cttgaagaaa tattgattgc acttagtata tcagcaacaa ataatcccac tgcagagctg
     6661 gcaatggaga agttaaagga tttaaaaggg tgtgaagtac accttactca tataccaacc
     6721 ccgggtgatg aaattggatt gaggcgattg aaagtaaatt tgacagcaga tgccaatttt
     6781 cctacaagag aacttttttt aggataataa tttttaagaa tgactttctt taatttttta
     6841 aattaagtaa attaggttat atttaaaaag ggtaaatact tacagaacca aaggtacaaa
     6901 acactggaat ttgaaaaatt ccagttgttt tttctggcgt tttttgccca gaacaagtta
     6961 taattacctt aaaccctcaa agattgaggg ggtgctggtg cgacgaataa ggagcaccga
     7021 ttagcacggg tggcgagatg gtgagccacc tatttagggg agttgatacc tcttggggat
     7081 tttaaagggg tttaaacccc tttaagaaag aaccggagtt gacaggaaaa acaaaatata
     7141 acaaaataaa aaaggaaacg gggttcataa atatttataa aaaaggagtg aaaatgaaac
     7201 tgaaaatttt tctttttgtt tttctattct ttatatcaat ctttttacct gcccagaata
     7261 taaaaatccc agaaaatgtt gagattatta aagatgttgt ttatggagaa gttgacggaa
     7321 aaaaattact tctggatata gtaaaacctg aaaaaaatga aggaaaaatg cctgttattg
     7381 tttttgttca tggaggtgcc tggagaggtg gagataaaca gggtggtatt tacagattaa
     7441 ttcaatttgc agaaaaaggt tatttttgtg taactataaa ttatagattg agtcaggaag
     7501 caaaatttcc tgcccagatt gaagattgta aatgtgctat aaggttttta agaggaaatt
     7561 cagaaaagta taatattgac caggacaaaa ttggtgtctg gggtccatct gctggtggac
     7621 atcttgttgc tttacttgga acaacaggtg atataaaaga atttgaagga aatggtggct
     7681 ggaaagaata ttcaagcaga gtaaatgctg tctgtgactg gtttggccct tctgattttt
     7741 taacaggttt tgaaaaatca aataaggagg gttcacctta taaagtaata tcagaactta
     7801 taggtggtga aattgaactg ttaaaagaaa aagcaaaaaa agcaagccct gtttattatg
     7861 ttaataaaga ttgtccacca tttttaatta tgcatggaga taaggataat gttgttcctt
     7921 atgttcagag tgtaatcctt tataataagt taaaggagaa taatgttcct gtaactcttg
     7981 ttaagataaa agatggagga catgggacag gatttaaaaa gaaggcaatg gattttgttg
     8041 aaagtttttt tgacttttat cttaagggtg aaaaagaaaa atggcagagt ttaagaggaa
     8101 ataatgattt tattgagata cctgctgaat aaattttact gagatatttt ttaagtttaa
     8161 aaagggggaa aaatgaaaat atttataata gggggtactg gacatatagg taaaaatctt
     8221 gtaaaaatgc tggaagagga aaaatttgac ctttttattt taacaagagg agttaaaccc
     8281 ctgcctgaga ataacagaat aaagtttttt aaaaaaaatt atgattcaaa taaagaggaa
     8341 tggaaaaata tatttgatga agtcaagcca gatgtggttg ttgatatact cgggacattt
     8401 gctccctgta tttatgaagt atgtaagatt tatattaaac actttattct ctgcggttca
     8461 atatggatgt ttggagagcc aaagaaagtt ccaactcctg aagaaactca atcaccttgt
     8521 attttttccg gttatgctaa aagatatctt gaaatgcaag agattaaaat ccagacaaaa
     8581 aaagatggag tgaactttac tgctattatg ccaccaaata tatgtggacc tggtaaaatt
     8641 cctcttgatt gttatggaga aagaagtatt gaaaaccata aaaagcataa aaagggagaa
     8701 attgtttttc ttcccgaacc tggacagaca cttatcggtc catgtgatgc tgaagatgtt
     8761 gcaaaaggtt ttttcctttc aattattcaa ccttcaaatt cagaagatga aatttttaat
     8821 gttggttcag aatatgccat tacagtgaaa aattttgttg aaacctatgg gaaaatctat
     8881 aatgtaaata ttcctgttga atgggtaagt tggagagaat attcagaaaa aataaatcca
     8941 caaccagggg caaatactca ttttaaatgg catatgtgtc ctgatatttc aaaaatttca
     9001 aaaaaacttg gatataaacc cagatataca ccggaagaaa caatggaaag agcggttaga
     9061 tggatggaag aacaaaatct gatttaaaaa ggagaaagtt atgaaaatta ttattataac
     9121 agaccttgaa ggagttgctg gagttgtgga ttttgagaca caggcactat ccgggagtaa
     9181 atattatgaa actgcaaaag aactcgcaac tgaagaagta aatgcaacct gtgaagcatg
     9241 tattgaaaag gacgtagatg agattctcgt aatagatggt catggtccgg gaggaataat
     9301 tcctgaaaaa atacataaag aagttaaact attacacgga aggccggtac cgactttttg
     9361 ggaacttgat aaaaaatggg atggtttaat tcttcttgga taccactcaa tgaatggaac
     9421 tgaaaaaggg aatttaaatc atacatatac acttgaaata gttaatatgt ggttgaatgg
     9481 agaaaagata ggagaaattg gaatgaagat atatcttgct ggctggtttg gaatacccac
     9541 aattttaata tcaggagatg aagcagcagt aaaagaagca gaaaaatatg tcagtaatat
     9601 tgaaaagtgt attgtaaagt ggggaataac aaaaacaagt gcaatttctc tttcaccttt
     9661 aaaagcaagg gagataataa aggataaaac aaaaagagca ttagaaagaa taaaagaaat
     9721 aaaacctgta aaattggaag gaaaatgtga aattgttatt gagtacttat ccactgcaga
     9781 tgcatttaat agaggcgaaa aatctgatgc tgaattaatt gaaccaacaa aagtaagaat
     9841 aaaaggtgaa aattttattg atgtatggaa gaaattttat agaggatgaa aaatttccct
     9901 aaaaaatgac acacatattc agtacaaatt gaagcaaact tttaaaattt ctcccacata
     9961 aactattcat gagttaattt aaatcttgac aggaattttt aaaatgtgta taatagtgta
    10021 tacaaattaa actttttttt gaaattcagg tctaaattat aaaatggagg tggagagtgc
    10081 ttaaaaaatt tgaaaggatt tttaaaattg taattatttt ttcttgttta tatttgttaa
    10141 atcttttttc atctgattct gtcagtgtta ttttcagtgt tccgattgtt aaaaatggaa
    10201 ttgaggtaaa agttggtcca aattctggag cggaaagtat taaaaattat gcaggaaaaa
    10261 caggactgga aacaaatatt ttaaaagaag caggtgtaat ttattgtgat attgatgata
    10321 aattcattta tgatggcata aataaagttt taataaaagt tgaatactgg gataaaaaag
    10381 ttaatagtga tgaaggattt tatattgcgt atgattcagt tggagaacca agggtttatt
    10441 taccgaagat atattttgaa ggaacaaata catggaaaaa aattgaatat tatgttgaca
    10501 atgctaaatt tgcaaacaga cagaatggag cagatttaag agttttttca ccggaaacag
    10561 atatatgtat aaggaatata actattgaaa aagtccctga aaaagtccca attaaaaatg
    10621 ttgagattgt cccttcttca attgttctta ttgagggaaa aagttttagg ccttctttaa
    10681 ttataaatcc atactatgca acagataaaa tttttaaatg gagttcagat aatccaaaag
    10741 ttgctactgt taattctgat ggtgttattg ttgctgttac aggtggaaat acaatcgtaa
    10801 gagcagtaag cagggatgga aaatttaagg cagaatgtaa tgtaaaagta atttctgtaa
    10861 aaggtaaagg aatccttgct gaaagagtta atgattttct tgatagtatt ggaataaata
    10921 caagtatatc aacaagagga gaaatttttg aaaaaacaat tgagtgtatt aagtatacag
    10981 ggataagatg gataagagga atggcaagtt tagatgaaat gaaaaaatta aataaagaaa
    11041 caggtgttaa atttacttta cctttaatac aggatataaa taaacttatt aaagttggaa
    11101 gagaacttgc gaaagacggg attttacttg gttttgaagg accgaatgag ccgaataact
    11161 ggggatttac atatagaggg gaaaaaggtg gaggacatgg agcaaataca tgggttccta
    11221 ttgctaaatt tcaaagagat tcttatggtt ttataaaatc agaccctgag ttaaagagat
    11281 atcctgtatg gaatttaagc gaaggaggag cagaggtaga taatgtagga ttgcagtttt
    11341 taacgatacc agaaggagtg gataccataa tgtcagcggg tacacagtat gctgattatg
    11401 cgaactgtca taattatgcc tgtcatccga cattacctgc attaatagac aatcaggcgt
    11461 ggtatgcagc agaccctgta ataaaagcag tacctgagta tgaggaaagg ggaggacatt
    11521 ttgatggact ttttaagaat tatggagaga catggttaaa gaaatataaa ggatataaag
    11581 aggaagagtt aaaagtatta ccgagggtga caacagagac agggataacg atagatgaga
    11641 gcagaggtat aacagaggag atacaaggtc ttttatattt acacatctat ttatcccagt
    11701 ataagagggg ctggagatat acatcaatct acatattaag ggacaggagt gatgagacag
    11761 gtaatcagac atttgggttt tacaggccgg attacagtcc gaggaaggct gctcattatc
    11821 tgcacaattt aacgagtata ttaaaagatg agggaaagga gtttatacct ggggggatgg
    11881 gatatgagat agaaggtcag aatgaatatg tacatgattt attattgcag aagagtgatg
    11941 ggagatttta tttgattata tggaatgaga gatttggaag taaaaaagaa gatgaagttg
    12001 tggttaaatt gagcaaaaga tataaagaaa taaagatata tgatgttgtg aaagggatag
    12061 aacctgttga aaaattaaaa gaaacagaca caataaaact caaacttaaa aacagtcctt
    12121 taattgttga aatttaaaaa atgaagaaag gaggtgaaaa tatgaaaaag ttaattctgg
    12181 ttctgacgat tgtttttggt ctggcttctt tgtgcagtgc agaggttatt tttaaaactg
    12241 acttttcaaa aggtgatttt gctgaccttg gatggatagt taaaggcgac tgggatattt
    12301 ttaaatatgg agtaaaaaat gaccctggac ctgttgcaag atatcctact aacaaacctg
    12361 atggaggaac tctaacaaaa aagtttgaag aagtgaagga cccacagaaa cttattcttt
    12421 catttgatta tggatgggga tggggtgcag aggaccaggg tgctgattct gtgagtataa
    12481 tgcttcttga ttcaaatgga gatggatata ttttttcaat tggaagatgg aaggcaaaat
    12541 ggtgttttca atggggaatt gtgaaaaatg gagttccacc aaaagaaaaa aagtgggcac
    12601 ctgaggatat ggattttaca cttaagtcaa taagagaccc gaattttgaa aaacttggac
    12661 atgtgattat tacaagagaa aaaggtggaa actggataat tacaggtgag aactggaaca
    12721 aagggaaagg tggtacaatt aaatttacag ataatacagt gagttctttc agtcaggttg
    12781 ttctctacgg aactaaaaac tttgatgaac aggtattcaa taatattatg cttgatgtga
    12841 caaagtaatt tttatttcct ggccctgaat aaaatttcag ggccaggttt tttaagaaag
    12901 gaaaaaaatg attaaagcta atgtaatggg tcttttaatt tcattaattt cttttactgg
    12961 ttgtacgcaa aaatttctta ataaagaaag caataaagaa aatgaaacaa aagtattacc
    13021 ggcaaaagtt gttccacaaa gagttcctca gggtagttca tttaaggttt attttaactg
    13081 ggaatcagat ggacctttaa gtaaaccata taaaattttt gttcattttc ttgatgataa
    13141 tgataaaatt gtatttcaag gagaccatga accaccttta atgcatacaa atcttcctca
    13201 atggaaaggg aaaataagtt atgaaatgaa atttaaagtt cctgaaaatc ttgctgatgg
    13261 ttcttataga attattgttg gactttatga taaagaagga agattgaaat tgaaagcagg
    13321 gaaaggtgta attgaagaaa cagatttaag atacagagtt ggcattttaa atgtagattc
    13381 aaaggcaaaa cctgttccac ctgatactgc tggaagaaaa acacttgatt taagtaacta
    13441 tgaacttgtt tttaatgagg attttgaaaa ccatttagat gtttctccct ggggacctgg
    13501 gacaagatgg attgctcata ctccatggca tggcgatttt ggagatgcga tatttatgga
    13561 cccaggcgtt tatcctgaat ttccatttaa ggtagaaaat gggattctga taattgaggc
    13621 aaggaaggat aaaaagtttg cagagaaaga cccctggaag agagaatggg catccggact
    13681 tttatgttca aatgacccca agggtaatgg tttctccctt caatacggtt attttgaaat
    13741 gagagcaaaa ttacccggag ggcaaggtgt atggcctgct ttctggcttg tttcaagtta
    13801 tgatagaact gataaaaatg cgggtaagga tggagcaata gaaatagata ttattgaata
    13861 ttatggagtt cctgatgcat acagttctgt aattcacata tgggaaccaa aaccacatag
    13921 aggggttgga acaagagtaa caaccaggaa aaatgaaata acagaggatt ttcataatta
    13981 tggttgtatg gtgacgcctg agtggattat aatgtatttt gatggtgttg aggtttggag
    14041 agtaaaaaca ccaccagaac ataataagcc cttaatgatt cttcttaatt tcgcacttgg
    14101 ctctggctgg cctattgata aggttccaaa ttcttctttt atgtatgtgg actgggtaag
    14161 ggcttatgca ccaaaaaaga aaaaatgaca aaaaaagaaa gagttaaaag aacaatttta
    14221 catcaagagg tagatatagt tccttttaaa attgatttta caatacctgc atgggaaaaa
    14281 cttatttcat ttctgggaag ggaaccatca attgatgaaa ttggtaatca tattgtagaa
    14341 attgaaccgg taacctctga cgcatggatt gagataaaac caaatttctg gaaggatgaa
    14401 tggggaataa tatggaatag aacagttgac cctgatatag gaaatccaat accagtactt
    14461 aaagaaccat ccttaaaaaa ttataaattt ccagaccctg aaagaaaaga aagatatgaa
    14521 aaatttcctg aatttataga aaaaaacagg gacaaattca ttcttgcaaa tattggtttt
    14581 tctctttttg aaagagcgtg gactttaaga ggtatggaaa acttacttgt tgatatgatt
    14641 gaaaaccctg attttgttca tgaacttatt gaagcaatta ctgatttcaa tttaagaatc
    14701 ataaaaaatg tttcaaaata tgaaatagat ggggtgagat ttggagatga ctggggtcaa
    14761 cagagaggac ttataatggg tataaaatac tggagagaat ttataaaacc atacttaaaa
    14821 attatgtatg atgaggttca taaaaatgga ttatttgttt ttattcattc atgtggagat
    14881 ataaaggaaa ttattcctga acttattgaa ataggagttg atgttttcaa tccatttcag
    14941 ccggaagtgg tggatatata tgagatgaag gaaaaatatg gtaataaact tactttttat
    15001 ggtggtataa gtattcagaa attacttcct ttcggaacac cagcagagat aaaaaaagag
    15061 gtaaataaaa ttcttgaaat tattggtaaa ggtggtggtt atattgcatc tccttcccat
    15121 agtatcccga aagatgtacc tgttgaaaat atagttgctt taattgaaac tctgaataat
    15181 caaaaagaaa aaaataaaat ggttgaaata aatgttttat aaattgactt gacaaaataa
    15241 aatgaatgtg tataataatg tatacaaagt ttacataaga gattttagaa aggatgagag
    15301 atggaaagaa ataatatacc attttacaag cagataaaag aggaaatttt aaaaaagata
    15361 gaaaaaggtg aatataaagt tggagataaa attccttcaa tttcagaact ttcaaaaatt
    15421 tataatgtaa gtaatattgt tgtcagacag gcacttggag aacttgtaaa agaaggatac
    15481 cttgagggta ttccgggtaa aggtactttt gtaaaagaaa aaagagaaga gataaaaaga
    15541 gatgaaaaac ttgttggact tatatttact gaaatggttg ggaatccatt ttttgcagaa
    15601 attttcacag gaatagaaag tgtattatca agttatggtt atcatatggt tatttctgta
    15661 agctataaca ttgtagaaag agaaaaaaga tttcttaaag aatatatgga aagaggtgtc
    15721 agaggaataa ttatcactcc gacagaagta aatagaactg taattgataa tgaaatattt
    15781 tctgaacttc aaagacagaa tattcctgtt atatttgttg atagaaaaat agagggaatt
    15841 gattttgatt atatttcttc tgataatgta gaaggtggtt atattgccac gaaatattta
    15901 atttcacttg gacataaaag aatagggata attcttggaa taaatgcaaa tacggtaaga
    15961 gatagattag agggatataa aaaggcactt caagagtatg atattatctt tgatccatta
    16021 cttgtaagaa gaagttttgc agaattacag tatgaagaag caggatataa caatacgatg
    16081 gatttattac atttaaaaga accaccaaca gcaatatttg catgtaacga agcaatagca
    16141 attggtatat atagagcatg ttttgaactc ggactgaata ttccagatga tttatctgtt
    16201 attggatatg ataatttacc tttcacctct tctttaaatc cacctttaac aactattaat
    16261 cagaaaaaaa gagaaatggg agaggaggca gcaagaatat tgatttcaag gataaacgga
    16321 gataaaagcg gacctaaaaa aattatattt aaaacagaac ttatagagag aaattcctgt
    16381 aaacaattaa aagaggagga acaaaatgaa aaagttagag ttaagaaaaa agagaattca
    16441 aaatgaaaaa aggaggtgca aaatgaaaaa gaaaggattt acattaattg agttgttagt
    16501 agtagttgcg ataatagcga ttcttgctgc tatgcttatg cctgctttga gtaaagcaag
    16561 agaaaaagca agacaagcgg tctgtatgag taatttaaag gaaattgctc ttgcttttca
    16621 tatgtatgca caggactggg atgattattt gccaccttat ggagatagtt atacaggtga
    16681 ctggtggact gtttggccaa atgttcaact ctgggcgcat aaaatagcaa aatatacagg
    16741 tacaaaaaca ttccagacat ataatatgcc aaagagtaaa cagggtgtat ggagatgccc
    16801 ttctgttctt gatagtaaaa ttggatgggg tggaacttat ggttatggcg ttctaactgg
    16861 ttctatatca ggaagtaata ttcattatgg attttactgg agttcaaaca gaatatttat
    16921 aaaactatca agaattccga ataagtatca tgctaaactt atgcttgttg cagatgcctg
    16981 ttgttttcca ccacatggga acacatcaaa cagagatagt ggagtcccaa tttactgggc
    17041 cacagtgaaa caactttccc aaacacctga aactactgct gcttctacta tatggtgtcc
    17101 gatatgtact tactggaacg ataacaataa ccccacaaga tatatttcaa acagacataa
    17161 tggtggtggg aatgtagcat ttttagatgg tcatgttgaa tggatgtctt tatttgatgc
    17221 tgttagaata agaaatgata acccatacgg atgctggagg gctggttatt cagaccttaa
    17281 aatgccacga caatagttaa aaaattttta aggaaaaaag atataatgaa agcatagtgc
    17341 accggcagta aaatatagta aaaaaagagg tgcgttatcc ttttaaagga ggaatgatga
    17401 aaaaatggtt gataatattt attttttgta cttttttgtt ttccgctgaa aagattgatt
    17461 tatttgaaaa agacaaggaa ctttatggat ggaaaattgg acttggtctt gaatttaaag
    17521 gagcaaaagg tcaacttatt attgatgata atgttaagta caatggaaaa ccatctttaa
    17581 aattaacagg agattttaca gaaggtggaa aatatgttga agtaagtaaa aatcttgata
    17641 atcttgatat agaaagtttt tccttttatg ttaaatatcc aaaagatgtt ttgactttca
    17701 gaattataga tagttcaggt cagtgccatc aaataaggat taaaattaaa aagacagatg
    17761 actggcaatt agtagaattc ccattaaaga aattttttga gaagatggga acatctgatg
    17821 cagtaagtgg aataatggga tacgaaaaat ggagtgggaa aaatgatggt agatggtatg
    17881 gacctgcaaa attcatctct attcttttaa gtcctgataa gaatacaggc aatatggtta
    17941 caatgtggtt taatgaattt actgcagttg tttcagagaa aaaagagaaa aaagagataa
    18001 taaaaacaat tcctcttgat gatttattaa aacttggaca gaaggactgg gatttatttc
    18061 ttggaacaga atttaaggga gcgaaaggta atcttgatat tgtcagggat ggagaagaat
    18121 actgtttaaa acttgctgct gattttacag aaggaggggc ttatatagat actggaaagt
    18181 cacttggatt tttaaatgta gatgcaataa aatcaatttc tcttaaatta aaatcagata
    18241 atgttaaatc atttggaata aggattatag atagttcagg tcaggtttta cagaaaaaag
    18301 gatttaaatt tacgcctgac aacaaatggc atgatgttgt tataaatatt gatgatttta
    18361 acggagcaga acactggtca ggagcaaatg atgggaaagt tcatcagcca gtaaaaaata
    18421 tcaggattgt tttaactacc tactcttcag aagataaaaa acctgttcta tttataaaag
    18481 atataaaagc agggatttta tttcaaggag aggtgtcagg aacagtttta gaagaaaatt
    18541 ttgaagatgt tgtaaatatt tctgaaaatt gggaaataaa aggaaatatc aatgttattg
    18601 aaggagatag tttcagcggt aaaaaaagtt tagtacttga aagaagtgaa aaaaatgtca
    18661 atgttgaaac aagtgcttta agtaagatat ttaatgttaa acccggtatt ttgagaattc
    18721 agggtgttgg aaaatcagaa ctttattctc ccgataattc ttactgtgga aaagtatctg
    18781 ttgaatttct tgatagtaat aaaaaagtta ttgaaagaca ggaagttatt atttttactg
    18841 gaaaaacaaa ctggcaaaca ttttcaaaag agatagagat aaataataat gccaattctg
    18901 ccagagtaaa aattgattta cagaaaacat acggaaaatt gaatatagat gatttaaaaa
    18961 tttcctatat atctccatct tcttccatag ttaaaaaagt tcagaggatt gagatttctt
    19021 cggaaagatt gggaaatttg ttttatccag aagacgaagt ttctctcaat attaatgtta
    19081 aaacaacttt acctttatct gatagtgaaa aaacattgaa atgcgaaata aaagattact
    19141 ggggagcaaa tcagacaaat tcattgcaat taaaacttga cagatttgaa attgataaag
    19201 gagtacttat ttatagtact aaacttaaaa ttaaagaaaa acttgaaata ggaaaatact
    19261 atgaaattca cattactgtt cctcaggaag gagaaccatt taaagaattt tcaacctttg
    19321 cgattttacc tgaagcaata acaaaaaaat ataaacctga agaaataccc tttacacaga
    19381 gaaactggga taatcgcata agagaatact ttttactttc tgaccgtctt ggaataagga
    19441 tatgcggaat atggagtgga tttgatttta aacctcctta taattcacat gctcctggtc
    19501 ttgaacttgt aaatgaactt ggaatgaaat gggttgcagg aactcctgga gcaggtgttg
    19561 aacataaaag aggtaactgg gaacaattta cagaagaagt tttgaaagaa gggatgaaaa
    19621 aatttgtgga aaaatataaa aataatggac tttattatat ttgtcttgga aatgaaccac
    19681 caacggatta tgaaaatgca gtaagaaata ctaaagcata taaagcaatg tatgaaggtg
    19741 taaaagaagt tgcacctgaa ttgattgtac tcgggacatc tgttggacct gctgaaaatt
    19801 attttaaagc aggttttcag aaatatcttg atatgtatga cttccataca tatggggact
    19861 atagagaaat cccgaggatt tttaaaaaat ataaagagtt gttttcaaaa tatggaggtg
    19921 aaaaaccaat ctgctcaaca gaactcggaa ttaacagtca gggtatgaca agatatgatg
    19981 tggcaaaagg tcttattaaa tccataactc tattttttgc ctgtggtggt gctcattgtt
    20041 cctggtttga ccttttatat cctgatcctc aaggaaaagc agaaacatca gccgcacagg
    20101 cacacaatgt tttctattgc aaatataacc tttatgctcc aaaacttgat gcaattgctt
    20161 attataacat tgtaaatggt atatgtgtta agaaatttgt tgaaagtaaa gtttatcctg
    20221 atggaacaga agtatatctt ttcagagata aggaaggtaa tacactttta gtcctttgga
    20281 atgacaaatc aaatagtgaa tttttctttc ctctaccaga agttgatgat gttgaagtta
    20341 taagaattga tggttcaaga agaaaattaa atgctggaaa gaatggaata aacttaaatt
    20401 taatacagga agaacctgtt ttgattttat acagacagaa tggtaaaaat attcctgaag
    20461 catttacaca gccaaaagta aggataattt caaaacctga ttatattatt gaaggagaca
    20521 gttttaaaat tgtttttgaa ggagaaaata tatctgttga tgagatggaa atttcatgtc
    20581 cttcagaatt tagcattaga aagatacaag atggaaaaaa aagagtaatt attgaagtaa
    20641 gtattcctga aaattttatc gcaagagaag gtagatttag aatttcaatg tataaagagg
    20701 ggatagattt tttccttaat ttaccagttg taagcaaaat tgaagggatt attttaccat
    20761 atccttcatg tttaaaaaat ggtccaggag taagattgat tttgaagaat aatggagaaa
    20821 aaacacagaa agtaaactgg gttgtttcta ttgaaaaaga gtttccgatg aaaaacggaa
    20881 catttaatct tcttgaagga cagattccaa atgcatattt taaggatatg acaaatggaa
    20941 cagaagaatt gaaaagcaaa gagggaaaag aaattttatt aagtatggaa aaagtagacc
    21001 ctttaacaat ttatcagata aaatgtgttg tgaaagatga atttggaaga ggatttgaat
    21061 ttaaaagata tataagtgga tttcttggtg ctccttacag aaaagaagaa ataaaaattg
    21121 atggagacct ttcagaaagt gactggaatt cagcaccaac agcatttctg gataaagaac
    21181 agcaggtgta ttatctgaag aaacttgaga cagaaatgtg gaaaggtatt gaagatttat
    21241 cggggaaact taaatttctt tgggatgaaa aatatcttta tattggagtt gaggttatag
    21301 atgatgtgca ttcaacacct tatgcagatg gttcaatatg gaatcaggat ggacttcagt
    21361 ttttatttga ccctaaaaga ggagaaaaag agaaaaaggg taagtatgat atttcaattg
    21421 cttctggaca gaaaggtctt caatgctggt ctcatcttac tgcagaccct gcaaaatcac
    21481 caacaggaga aataaaggat gtaaaaatag gatttaaaag aatagataat aaaacaattt
    21541 atgaaatagc aataccttgg gataaacttt ctccttttta cccttttcct ggagcagacc
    21601 ttggagttgc aatgattata aatgaagatg atggaaaagg aagggtaagt tttattggct
    21661 ggtttggttg tgttcattcc aaacaaatgg atatggttgg agatttgata ttacttgaaa
    21721 aagaaaagtg acaaagagag catttatttt aggagttatt ttttctatat ttgttggagt
    21781 tggtgaaatt atttgggttg catggacaag tggttctcct atgacaaatg accattctgc
    21841 aggtgcctct attttctttt tatttattct ctgtttattc gtaaatccag ttctaaagaa
    21901 aatcagaaaa aattcagcac tttcttcatc agaacttact cttatcttta taatgatgct
    21961 tgttgcctgt gcaattccat cctggggact tgtgatgaat ttgatatatt taattgcagg
    22021 tgttcattat tttgcaactc cggagaataa ttggtttgaa ataattattc caaaaattcc
    22081 aaaatacctg acaccaaatg atcaacaagc aattaagttt ttttatgaag gattacctgc
    22141 gggtgtttct attccatgga atgcatgggt tcaacctctt attgcatgga cattattcat
    22201 tttggtactc tcttttatga tgatttcaat ttcagttatt ttcagaaaac agtgggaaga
    22261 aaatgaagcg cttttatatc cattaatgat acttccttct gagatgataa aagaaggtaa
    22321 aggtataatt tctccatttt ttaaagatag attgatgtgg cttggttttt caatatcatt
    22381 catacttaat tctctcattg ttttacgttt ttatttccca ttggttccta aaattgtact
    22441 tggtaaatgg attggttttg gacctggagg agcacttggc tggttgagta taggacttcg
    22501 ttttgaaata ctcgggattg cttttctctt aagttctgat atatctttaa gtatctggtt
    22561 atttacttta ttttattttg tggaggaagt gatttttaat agaattggat tgaatatagg
    22621 ttctcctgat gcttattctc ttgctgtacc tattgcctat caaatggaag gagctttttt
    22681 ggtatttatt ttatttagtt tatggataag tcgttctcat ttaaaaaaag tatttaacag
    22741 aatttttaaa aatgatactt caatagatga ttcaggagaa gcacttacat ataaacaggc
    22801 atttatcgga cttattcttg gatttttgtt tacagtttta tgggcaaaaa tgacaggttt
    22861 taacctttta tcctctgttt acttttcagt agttttaatt cttacattta ttggaataac
    22921 taaaattgtt gttcagacag ggacagcata ttgcagacct ccaatcaatc caggtgtttt
    22981 cacacttcat tcttttggaa gtagcaggtt aggttctggt agtgctgtaa gttgtgcatt
    23041 tacatttcct tatgcaggtg atatcaggac ctctgtaatg acaacatctt caaatggatt
    23101 gaaaattgct aaaaatttta atttgagagg aagattttta ttctgggcta tttttacagg
    23161 agttctggta agttttattt catctgtaat aactgttata gtaataagtt ataaaatagg
    23221 agggataaac tgctggggat ggcaaccagt tggaatgcca ccatatacct ttaactgggc
    23281 aaaggaaatg attttacact atcagaatgt tgatataaga agaataggat ttttactttt
    23341 aggtggattt ttaatgtttt tattatattt tttaagaata aggtttgtta attttccggt
    23401 acatccaatt ggtatggcat ttacatataa tgttccaact caatgggtat ggttttcatt
    23461 ctttcttgga tggcttttta aaagtatgat tttgaaatat ggaggagttt ctgtttacag
    23521 aaaacttctt ccattctttt ttggtctgat tataggtgct ttcttttcag caggattgtg
    23581 gaatatcata aactttttca cacatcaaca gggaatacat ctaaccgtta cttaaaaagg
    23641 agaataaaat gaaaagagtg acaattggag caccatggca tgttttgtat tactatgaca
    23701 ggaagtatca taattatttg agaaagataa aggcaacagg aattgagaca tatgtaaaat
    23761 ggtcagatat agagaaagaa cccggacaga ttgatttttc aaactttgat agacaacttg
    23821 agattatgaa aaaatatgga gttaaatggc aaccatttct tatttgtgga ccatggtatt
    23881 cccttcctta ctggtttcat aaatcaagtg actgttatta ttttaaatgt cttgaacaca
    23941 gtattgaaag tggaattcaa tcaatctgga atccctattt taagaaatat gttgtgagat
    24001 ttttgaattt attttatcaa aactataaat caaagatgga tttaatagat tctcttcttc
    24061 tggggataag tggtgactat ggagaagcaa tttatcctgt tgttggaaac tgggatggta
    24121 tgtatcatac ccataaagga ttctggtgtg gagacgattt tgctataatt gatttcaggg
    24181 attatcttaa agaaaaattt gaaaaaatag aaaatctgaa taaaagatgg aatacttcat
    24241 atagaagttt tgatgaaata aaaccatttt taaaggaaaa ttcaccttca aggcccgcaa
    24301 tgatagatat ggtttactgg tatcgtactt caatgataaa atgggttgaa ttctgggtga
    24361 aagaggcaag aaaattgtgg aaggaaaaag atatttatct tgttatgggt ggagatagtt
    24421 ctgcaaaaga aggacagcat tatttcaaag cagcggaaat ctgttcaaaa tataatgttg
    24481 gaataagaga tacaaacgca agagataatt tcccatatct tcatacatat caggcaaaaa
    24541 catcagttgc aacaaatttt tatggaaact attgcagtta tgaatcttct tctggctcaa
    24601 atgaaaaatt aatagttgca agaattttta cttttattat tacaaatgca agagagtttc
    24661 atgagtattc ttttcattca aatgaaaaag ttattaataa ttttaaaaaa tacagaaaac
    24721 ttatggaaat aaattttaaa agaaaagttg atattgcagt attttatcca gaaccttatg
    24781 taaactggat acatgaatat gcaatttcat gggaaataaa aaatcttcca tggggtcttc
    24841 cacctgaatt acacaattcc ttttataatt taagatacta ttttgatttt gatattgttg
    24901 atgattcttt aatcagaaat ggaattcttg aaaaatatag ggtactgata atcccttctt
    24961 tttcaattat tgaagatgac ataaaggaat taatagaaaa caggataaaa aataaagaaa
    25021 aaataatagt ttttggaaaa gaaacgccag aggatgttta tggaaataaa ataaattttg
    25081 gagaagaaaa ccatattgta tcgttgagaa aacttttgaa gattttgaag aatatgggaa
    25141 ttaatgtaaa agaaagaaaa gatggaattt ttgaagtttt tgatgaagac ggtaggataa
    25201 tctgttatga tgaaataaaa aataaaattt tctggaaaag gagataaaat aaatgaaaga
    25261 aagtagaaaa gttgttgatt ttcttaactc tcttggtgca aatagtgctg tttcaaggag
    25321 agatgaaatt ttatcaaaaa caattgaatg tgttaaatat cttggaataa gatggattcg
    25381 ttcaggctat gaaagtaatg tccctgttga agacctactt gaattacaca gacaaactgg
    25441 tgtggttttc agttatggat tactcagtgg aggaaatgat ataaacagat tacttgaagg
    25501 agggagggct ctggcaagga atagggcttt acttgctttt gaaggaccga atgagccgaa
    25561 taactgggca gtaaaataca gagggaaaat tggtggaaga gattattcat ggatacctgt
    25621 tgcggaatta cagagagatt tatatatgtc tgtaaaaaat gaccctgagt taaagagata
    25681 tcctgtatgg aatttaagcg aaggaggagc agaggtagat aatgtaggat tgcagttttt
    25741 aacgatacca gaaggagtgg ataccataat gtcagcgggt acacagtatg ctgattatgc
    25801 gaactgtcat aattatgcct gtcatccgac attacctgca ttaatagaca atcaggcgtg
    25861 gtatgcagca gaccctgtaa taaaagcagt acctgagtat gaggaaaggg gaggacattt
    25921 tgatggactt tttaagaatt atggagagac atggttaaag aaatataaag gatataaaga
    25981 ggaagagtta aaagtattac cgagggtgac aacagagaca gggataacga tagatgagag
    26041 cagaggtata acagaggaga tacaaggtct tttatattta cacatctatt tatcccagta
    26101 taagaggggc tggagatata catcaatcta catattaagg gacaggagtg atgagacagg
    26161 taatcagaca tttgggtttt acaggccgga ttacagtccg aggaaggctg ctcattatct
    26221 gcacaattta acgagtatat taaaagatga gggaaaggag tttatacctg gggggatggg
    26281 atatgagata gaaggtcaga atgaatatgt acatgattta ttattgcaga agagtgatgg
    26341 gagattttat ttgattatat ggaatgagag atttggaagt aaaaaagaag atgaagttgt
    26401 ggttaaattg agcaaaagat ataaagaaat aaagatatat gatgttgtga aagggataga
    26461 acctgttgaa aaattaaaag aaacagacac aataaaactc aaacttaaaa acagtccttt
    26521 aattattgaa attttttaat ccctttttat acctattttt tcaaggtttt ctttcaagca
    26581 attcccaatc cagagttcaa gtccttctac aagataattt ttctgaaaat ccatgcttat
    26641 attatcaagt ttctctatta tctcctcggg taaatttttt ccaaatgctt ttatattttc
    26701 ctcaaaatgt tttgcttttc tcatccctac aagtgcagag gcaatataat tttttgatat
    26761 acaccagttt atagaaatct gagcaatagt catatcaagt tcttttgcaa ttttttctat
    26821 ttcctcaacc acttttaatg cttttgaata aacaggttca ttaaacaata cattggcttt
    26881 tctcacattt tcaactattt cacttctatc tttaaatctt cctgtcaaaa gtccttcagc
    26941 gagtggactg taagcaagaa aatcaatttt attttttata caaaaatttg ttgttccatc
    27001 aatatcataa catctccata ataaagaata tggaacctga tttgatacaa taacagaaga
    27061 gaaaatttct tcaggaaaat ttaaaagatg ctttttcctg aaattactta ctcctgcgta
    27121 tcttatcaaa ccttctttct gtaattttgt cattgagtca gcaatatcat agtaatcttt
    27181 ttcattcata tcttctttat gccataaatg tttcatttta ggccagtgaa cctgatataa
    27241 atcaacaaaa tcagttttca atctttttaa actccctgtt aaatgttctt ttacagtttt
    27301 ataatcaaaa tggtttcctc caatttttgt tgctattaca aacttatctc ttttcccttt
    27361 aagaatctct ccaagaacag attcactttc tccatacgcc tccgctgtat caataaaatt
    27421 aactccattt tctattgcac tatctattat tttttcaact tcttttttat cataatttcc
    27481 ccactgacca cctatctgcc atgctcctat tgccattgca ctaaccttta tatcactttt
    27541 cccaatagtt ttatatatca ttttttcctc ccttatttac ctatacagaa atttgaaaaa
    27601 atttcatcaa gtattttatc actaatttta tttcccatca attcatcaat ctggtttata
    27661 caacttttca aattttcggc aataatttct ggataggtct cctttaatgc ttcttctaaa
    27721 tattgattta ctgtgtttat ttttctttcc tgccttaaat tcagatatat ttcatcacct
    27781 tttatttctc catatccctc atttatgagt tgatacaatt tattttctat ttcctctata
    27841 cctgtattat ttttaaccga tactttcaca attctatctc tatcaaactc tctcaaaatt
    27901 ttatccattt caactttttg aggaaggtca attttattta ttataatcag atatggttta
    27961 ttttttatgt gttccagaag ttggtaatca tattcactca attttctact tccatcaatc
    28021 ataagcaagt ttatttctgc ctttttcatc cattcaattg atttttcaac tcccatcttt
    28081 tctattttat tttttgcctt tcttattcct gcagtatcaa ttatatttat cggtattcct
    28141 ttaatgttaa ctatttctgt aatcacatct cttgttgttc caggaatttc tgttactatt
    28201 gctctttctt cttttaataa agcattcaat aaacttgatt ttccaacatt tggacttcct
    28261 ataattgcaa cttttacccc tgtttgtatc aatttacctt tattgcactt taataaaaat
    28321 ttttcaattt cctgataaat ttcttttatt tctttttcaa tatttaacct gtattcttca
    28381 acatcttctt cttctggaaa gttaattttt gcttctatat tcacaagaag attaaatatt
    28441 ttttccctta tttcagaaat aaaattactt aaagaccctt ttaatttttt tattccaatt
    28501 tcaagtccag tttcagtttt gctgtaaata atatcaagta tactttctgc ttgagttaaa
    28561 tcaattcttc cattaagaaa tgcccttttt gtaaattctc ctggctgggc aagccttgct
    28621 ccctttttaa tacataaatt taaaacttct tttaaaggaa taagcccgcc gtgacatccg
    28681 atttcagcca tatcttctct tgtataactc tttggacttt tcataataac aactataact
    28741 tcatcaataa tttttccatt atcaacaata tgtccgtagt gaactgtatg tgagggtatt
    28801 ttttcaattt taaatttttt ctttaaaggg gctttcccag gaacaaaaat ctcctctatt
    28861 atcctgtatg tttcatttcc acttaatctt acaataccta tcccgcttat accaagtgga
    28921 gttgaaattg cacaaattgt atctttttcc atatcttata ttttacccta ttttgtgttt
    28981 aaagattatt gagtaaaaaa atacttctta aaagttggaa gggaagaact ggaaaaacaa
    29041 ggtaggaatc aattgaaaaa tcaatgatga aattttgaat tttatcattt atttctggaa
    29101 taagaattaa aaatttacaa tccttaaatt tttctttcaa tttttttaca acctttttta
    29161 tttctatacc gcattcatct acttcaatta tcactatatt tacattttcc agtttatcta
    29221 ttttttccat caatttctta tcatgcagaa gaaatccagt cgtttcatat tcgtaatttt
    29281 tcataagaaa aagatacttc tcaagcaaat tcttttcttt cattaaaaat aaaatctttt
    29341 tcatatcaga aatattatac aaaaaaaata accaaaagtc aataaaaaat tatataactt
    29401 ttagtttgtt taaaataagt gattttaact gtataattag aaaatggaag atttctataa
    29461 aaaaatcgga agaaaaataa gagaagtaag aaaatcaaaa aagataacac aggaagaact
    29521 tgcttacagg attggggtaa ctcctaattt tatcggtctt attgaaagag gtaaaaaaag
    29581 gccgagtata gaaacattaa gaaaaatatc agatgctctt gaagtcccag tttcttattt
    29641 ttttgataaa attacttaca aattacctga agaagacata ataacaaaaa agataagtgc
    29701 aatattaaaa gatacaacag aggaagaaaa gaagggaata tataaatttt taaagtcaat
    29761 tgtcagaaag aaaaaataag tttatattta acctctcctt tcttgtaaag ttgtaaaaat
    29821 atcacaattg atttaaaatt caaaatatgg gagaaataaa aaaaatccat cctgttaaaa
    29881 ttttctgtgg tttaatttat aaggaggagg aaattctgga aaaaacaaaa ataaaacttg
    29941 cagaaaaatg gggaaaaatt gatattgaag aagggccatt taactttgat tttacagatt
    30001 attatgaaga agagatgggg ataaatttaa agagaaaatt tgtatcattt gaaaatcttt
    30061 atatccctga agatgtttat gagtggaaaa tctttacaaa tgaagtagaa aaagaatttt
    30121 ctgaagaaaa taaaagaaaa ataaatattg acccaggata tattgacagt tcaaaagtaa
    30181 ttttattatc tacaaaggac tactatcata gaatttacat aggaaagggg atttatgcag
    30241 aacttacact ttattattca aaagggaaat ataatttttt aaactggaca tatcctgatt
    30301 acagaacaga aaactatatt tcattttttt taaagatgaa gcaaaaatat acaaaacagg
    30361 taagagaatt tttgaaaaat gaaaaaaatg tgggtgagaa tacttgttga tttttttata
    30421 attgtattta ttttgagttt aatcacagga attagagcgg ttatgccacc aagaatggga
    30481 atttttatca cacctaaaga tttaggactt ccttatgaaa atataatact tcaaacagag
    30541 gatggaaaaa gattgaaagg atggtttatt tattcagaaa aagcaaaatg tgtaattata
    30601 tgtcttcatg gttatcctgc aaataaatca gatattctac ctgttgttga gttcttatat
    30661 ccagaatttt cacttttact ttttgacttc agggcacatg gagaaagtga agggaaggta
    30721 tgctattttg gattgaagga atttcttgat gtaaaaagtg cgattgtttt tataagaaac
    30781 aacgagaaaa ttaaggattt accgattgga atatggggat actcttttgg aggtgctatt
    30841 gggattattt ctgcttcaaa gtataatgat ataaaatgta ttgtaagtga ttctgctttt
    30901 gctaatttcc ctgatatggt aaaaagttat tataaaaatt taggtccatt aaagtatatc
    30961 ttctcttctt tttcaatttt cctcggtaga tatgttttta gaagtgattt taaattgaac
    31021 agtcctgaaa attttatagg aagtgtgaaa tgtcctattt taataataca ttcaagaaat
    31081 gatgagtttg ttccatttac acatgcaaaa aaattatatg aaaaagcaaa atccgaaaaa
    31141 gaaatgtatg ttgttgaagg ttctcataca ggtcttgata gagcatatac aaatgaatac
    31201 agggaaaaag taaaaagttt ttttgaaaaa aatcttattt ctgaaaaaat tgaaaaaagg
    31261 aggcacaatg aatgaaattt ttaaaaggca tcctgaaaat cctattctta caccagaaaa
    31321 atggaaatat ccatgtaatg cagtttttaa tcctggtgtc atagaatttg aaggttatgt
    31381 ttatctttta tgcagagtag aaacatttga tggatattct catttcaccc ttgttaaaag
    31441 taaaaatgga gtaaataact gggaaatccc tgagaaaccc actttagttc ctgatgagag
    31501 atatgatgag gaaaaatggg gaatagaaga cccgagaatt gtatatttaa aagaaataga
    31561 aaaatatgca ataacttatg tttctttttc tcctggtggt ccattaatct ctttaatttt
    31621 aaccgatgat tttataaaat ttgaaagaaa aggacctctt gttccaccag aagataagga
    31681 tggttcttta tttcccagaa aaataaaaga taagtttgct ttaattcaca gaccaattat
    31741 aagaggagaa ggacatatct ggatttcata ttctcctgac cttattcact ggggaaacca
    31801 taaaattctt ttgcctgtaa gagcagggat gtgggactgt cacaggatag gtcttggttg
    31861 tccaccgatt gaaacagatg aaggatggtt gataatttac catggaataa gatatactgc
    31921 ctctatcccg atatacagag ttggacttgc tttacttgat aaagaaagtc ctgaaaaagt
    31981 tatttcaaga acaaaaaggt gggtttttgc tcctgaaaaa gaatatgaaa ggataggaaa
    32041 tgctatgaat gttgtttttc caacaggata tataattaaa gataaagaaa tcttcattta
    32101 ttatggatgt acagataaat gtatttctct tgcttttgct tcaatttcag acattttaac
    32161 catactactt aaagaaaaag aataagtgga atagattttt atcttaacaa ttccttttta
    32221 atttttccac ttcctgtttt gggcaattca tttctgaatt caattattga tggaacttta
    32281 tatgtagcaa gatgttctct gcagtaattt tttatttcag attcagtaag ttcttctcct
    32341 tcttttggaa caataactgc tttaatcgct tcccctctta ttttatctgg aactcccacc
    32401 actgccactt ctttaacctt tggatgtttg taaataactc cttcaacttc agtagcataa
    32461 acattaagtc ctccaacaat aataacatcc ttttttcttc caactatata aaggtaccca
    32521 tcattatcaa atttcccaag gtctcctgta taaagccatc catctctaat tacctgagcg
    32581 gtcaaatctg gttgattata ataacctggt gtaacaggcc atcctttcat aataatttct
    32641 ccaacctcac ctttgggaac ttctactcca tcctcatcaa ctatttttac ttcaatccat
    32701 ggaacaggtt ttccaacaga accaaatcta acatcttcta atggcggtaa aacatttgga
    32761 gccgctgtct ctgtaagtcc ataaccagaa agtaattttg cttgtgggca taccttatta
    32821 aattttttta ttacatcagg agaatatgga gcaccgaaaa cagcagccca tcttaattta
    32881 ggtaaatgaa atttctcaaa aacttcaaga tttaacatac cgataaacat tgttgggaca
    32941 agaaaagttc ctgca
//
LOCUS       DAOSAT010000008        32021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_1027_length_32021_cov_4.843865, whole genome shotgun sequence.
ACCESSION   DAOSAT010000008 DAOSAT010000000
VERSION     DAOSAT010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 32021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32021
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1027_length_32021_cov_4.843865"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PLF90_01340"
     CDS             complement(<1..960)
                     /locus_tag="PLF90_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOK55960.1"
                     /translation="MESKYNPLEIEEKIYKMWEENSYFYVKTDNKKEKYVIVIPPPNI
                     TGFLHMGHALNNTIQDILIRWKRMEGYNSLWVPGTDHAGIATQNVVEKELAKKGLTRK
                     QLGREKFIEEVWKWKEKYGSRIIFQLKKLGASCDWTRTKFTMDETLSNAVKEAFVRLY
                     KKGLIYKGKKIINWCPRCETALSDEEVEYKDEKSNLYFIKYPLLRGGFIEVATTRPET
                     MLGDTAVAVNPEDERYKNLIGEKVKLPFVERIIPIIADDKVEIEFGTGAVKVTPAHDP
                     NDFEIAQRHNLDFILIMNEEGIMNENAGKFNKIDRFECRKKVVE"
     gene            complement(962..1258)
                     /locus_tag="PLF90_01345"
     CDS             complement(962..1258)
                     /locus_tag="PLF90_01345"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOK55961.1"
                     /translation="MFIGTLKFEIIIPGSSSLKDKRRVIQSLQNKIKSKFNVSIAELE
                     HQDKWQRTVIGLCFVNSEKKEIENICEKIKNMIFDNSDIMVINELSEIIYLGEK"
     gene            complement(1278..2426)
                     /gene="larC"
                     /locus_tag="PLF90_01350"
     CDS             complement(1278..2426)
                     /gene="larC"
                     /locus_tag="PLF90_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOK55962.1"
                     /translation="MKKIGYFEVFDGCAGDMLAGSLIDAGLDIEILKKELTKIPLKNY
                     RIETKKVKRKTKFGHAIEGTQFLVIPVEKWEEETSYKDILKIIEKSSFSIIIKEKIMK
                     IFDILAEGESIVHKEEKEKLHFHQVGQIDAIIEITSVVLGIKLLEIEKVFSSAVGVSN
                     IAPATIEILKGIPTVLKNVPQEITTPTGAAIIKGISSFTPIVSDFYISKVGYGAGTRE
                     EPLPNMLKFLMGEIKQENESVMIIETNIDDMNPVLIGNLFEKLYRKGVFDVSIFSGIG
                     KKNRPVFKIEIILPEEKFNEVINILFEETTTIGIRCRKENRVILEREIKEIDTEFGKI
                     KVKISYMDNRIVNISPEYEDCKKIAEEKQIPLKTVYQKVYKNLENNLK"
     gene            complement(2423..2728)
                     /gene="yajC"
                     /locus_tag="PLF90_01355"
     CDS             complement(2423..2728)
                     /gene="yajC"
                     /locus_tag="PLF90_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOK55963.1"
                     /translation="MNLTMAQATPPQASPLGALLPLILIFFIFYFLLILPQQKKAKLH
                     KKMLDELKEGDKVITVGGLIGEVEKIKEDIVTLRFKEETKIDVVKNAISQVMPRKKE"
     gene            complement(2740..5151)
                     /locus_tag="PLF90_01360"
     CDS             complement(2740..5151)
                     /locus_tag="PLF90_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK55964.1"
                     /translation="MEKYIVDVKYLKEKYDLKFLNFETTEEIPPLEGIIGQERAKKAL
                     EFGLKIKSKGYNIFVTGITGSGRTTSVEQAVKKIAEKGKTPDDWCYVYNFTNPDNPNV
                     LRFPPGKANEFKKDMEILVNELKVELPKTFESEIYEEHRNQIIKEFQNKRNQLLDQIE
                     KTAKTSGFQIKQTPSGIVFIPLIEGQPIKEEEMEKLTEEAKTEIRKKQEMLYEELNDA
                     LRKIRDFEKEAKEKLNQLEKETAKFTISPRIQELKEKYKKFSDVCKYLEEVEKDMIEN
                     IDDFKEKKEIELLPGLKIPDRESSLYRYTVNVLIDNSQTKGAPVVKETNPTAYNLCGR
                     IEYRPHFGAMVTDFTMIKPGSLHKANGGYLILHILDVFKNYYSWETLKRALKNNEIII
                     EDLNEQFRLINTPTLRPNPIPLDIKVILIGHPVFYYLLYTYEEDFRKLFKVRADFSTI
                     MEKNEEGYKNYASFISKICKEENLKHLDKFAVGKIMEYGSRIVEDRNKLTTRFIEIAD
                     IIREANFWAEMDNSPIVREKHVKKALEEKIYRSNLIEKRIEELIKEGTIMVETDGEKV
                     GQINGLSVISLGDYSFGKPSKITCNVFTGKGGVINIDREVKLAGKIHNKGFLILNTYI
                     NEKYGQEEVISFSASICFEQLYEEIEGDSASSTEIYVLLSALANIPIKQGIAVTGSVN
                     QKGEIQPVGGINEKIEGFYYTCKSKGLTGKQGVIIPERNLKNLILNDEVIEAVEKGLF
                     HIWAVKTIDEGIEILTGIPAGEKRKDGTYPEGTINYLVSKNIKKYTEKYLKSIGKKES
                     KSKNN"
     gene            complement(5220..5774)
                     /locus_tag="PLF90_01365"
     CDS             complement(5220..5774)
                     /locus_tag="PLF90_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOK55965.1"
                     /translation="MRDKFDILICGVGGQGIILCSNLIGNAAVIEGISVKGSEIHGMA
                     QRGGSVEAYVRLNCNFGPKIPVGYADLIIGFETMEACRSSFYLKKDGIMIVNNFPIPI
                     PGKEYEYEKIIEFLKSKTKNIYIENFTKIAENIGSPKVINTLMLGFASKFIPLKKQSF
                     VEAIKITLPEKILKINLDAFNYFL"
     gene            complement(5764..7527)
                     /gene="iorA"
                     /locus_tag="PLF90_01370"
     CDS             complement(5764..7527)
                     /gene="iorA"
                     /locus_tag="PLF90_01370"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOK55966.1"
                     /translation="MEKKYLLGNEAIAYGLYEGGVNASFAYPGTPSSEITEALLKISK
                     NDVYIEWSVNEKVAFENAYGVSLTGRRSSAIMKHVGLNVASDSLLTSAYTGIVGGFLI
                     IVADDPFAHSSQNEQDSRRYAQFAKLVCFEPSSIQEAKDTVIFSFDFSEKYGLPVLIR
                     SVTRLSHGKADVLIGKREEKNSTSTFIKNPSQFVMVPSNVRKVIINLNKKQEVIKKDI
                     EKLKINKIDNGEGSTGIIASGLSYIYVKEFLNLKKKSIPVLKIGSYPIPEKKLGKFLK
                     GLKNVFVFEEGDPVVEEFVYLCSKKYNPEIKIKGKMSGDVKKEGELSVEDIEYSFSGK
                     RKNVYVNINLPERTPTLCPGCPHIGSFYILKKVFGKESIFPGDIGCYTLGIRFGTIDT
                     CLCMGSGISIGTGISRFEKKYIISIIGDSTFFHAGIPGLINAVYNHSNQVIAILDNRT
                     TAMTGYQPHPGTGINAKGDKTVEIDLKKLIEGCGVKNLVEVDPYLIKDSVEKIKQIKE
                     KEGVKVIIFKRECIHISKIKKTEFYIDKNICKKCKLCLEFGCSGIILVKDNIEIGNLC
                     NGCGICEQICPFGAIKKKNER"
     gene            complement(7577..9037)
                     /locus_tag="PLF90_01375"
     CDS             complement(7577..9037)
                     /locus_tag="PLF90_01375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOK55967.1"
                     /translation="MNRKIKFFLFVIFFLGFFFRVLHLGKHSLWCDELLTISLGKHSI
                     KWMIDYITYNDAHPPFFYLLVHFWLKFGENEIILRILPMLFGLLCIPYSYILGKKFYN
                     EKIGLFLSLFVSLSPPFILWSQLIKSYSLLTFLTILSFIFFIEILNSENKKFLPIFCL
                     SNILILYTHNFGFIVILIQILTLLILKKLNRKSFLLFFLIFLIYIPWLLKIPYQMNFT
                     LGVRRPFPALLKLPYTLFYFFFGETLSPFNFKILIPAFITYSIIFTIGVKNLLKLQKE
                     IKVLLSISLIFPLFFVFFPSTVPQNLIFISFFWYLLFSIGMHNFNYKNLLIYLNFLFL
                     LPSLIFYYTDNISQYHDTSKLILFREIFKEIQKIEKDGDIILTTEKYDREILTPLMWY
                     YSGKNKVFRINDKNDIDEVIKIFNREKKKSRFFLILDFINNPEISKEIREFFENKYEK
                     LYEKKYIYNEKLLSRLKGVKEYYYLVEVYIFQPKIF"
     gene            complement(9030..9791)
                     /locus_tag="PLF90_01380"
     CDS             complement(9030..9791)
                     /locus_tag="PLF90_01380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOK55968.1"
                     /translation="MVKCPLCEKNTNLKLKIKNYQIYNCKKCEISFLYPFPENIEKIY
                     DKKYFEKWYLPYYDDRKKYFERLYLKLKNFIPEKGKILDIGCGVGILMKMFEEKKYEV
                     IGYEISKFAINFCKENNLKVFDNFNFPEKSFDIITMMDVIAHVKNPLFYFEKSKKLLK
                     EGGILIIKTPLHSNYMFFLAKLFSFTPKSKSILHIPAQIYHFNKKSIFEIAKMKNFKV
                     EKVFIMKEFINRKFSILNVWKFFIEKSVVVILKNE"
     gene            complement(9793..10470)
                     /locus_tag="PLF90_01385"
     CDS             complement(9793..10470)
                     /locus_tag="PLF90_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK55969.1"
                     /translation="MKLSVIIPVFNEEKTIMKVVEKVKKVPFEKEIIIVDDGSNDKTR
                     EILKNLKDKNIKIIFKEKNEGKGMAIRKGLEYITGDVVVIQDADLEYEPMDWLKMIKL
                     IEEKKAEVVYGSRILGKGKKSSLFFYLGGRILSILANLLYNAQITDEPTCYKMFKKDV
                     IKSINLKCKGFEFCPEVTAKVRKKGYKIYEVPIHYNPRTIKEGKKIRLKDGIIGIWTL
                     IKYRFIN"
     gene            complement(10472..11656)
                     /locus_tag="PLF90_01390"
     CDS             complement(10472..11656)
                     /locus_tag="PLF90_01390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOK55970.1"
                     /translation="MERVSERKNLIYLLVIGFLLRLIFVLTLKNQFYFDDEYEYFKMV
                     QNFIAGKGLIVGENLKSFRPPFYPFLLSIFTLLKINLTGIRIIQTLISTFTIYLIYNI
                     GKETFNKKIGLWSGWISAFYPYFIFYNGFILTETFFIFFTVLSIFFLIKIVERNGKKS
                     FLTGIFMGISGLTRPIFQLYFPVSLIHIFFLKENFVKKIKKIFFITIGFCLTLSPWIL
                     RNYRIFHGFIPGTTMGGWVFWEGNNPYSEGGPCSHFPEGILEVDEITRDKILYRMTFD
                     VIRKNPERFLWLIQNKFRRFWNIIPNAPEFERKILYRFISVFSFGIMMPFFIIGFFIS
                     LKNKKAQFFHSLIILFTVFHIIFLASIRYRVPIEPFYIILSTYGFSYIILMVKNLFIQ
                     RG"
     gene            complement(11626..12318)
                     /locus_tag="PLF90_01395"
     CDS             complement(11626..12318)
                     /locus_tag="PLF90_01395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOK55971.1"
                     /translation="MILAYHRINPWYNDALTVKPEYFKKQIEYLISKKFLIVPVEEYL
                     KENKKILKRKIVITFDDGFADNFWFAFDILKSFRIFPLIFLIVDYTGTNKIFSRYKDK
                     EKDRFLNWKEINEMIKEGVEIGSHTLTHPHLTQISEKTAKEEIISSKKIIEDKTGKEI
                     KFFCYPYGEFNEKIIDFVKLAGYKGAFVTPKRGQKIKWTDFTMKRIGIYGHNNFFIYK
                     LKIWKEYLKGKI"
     gene            complement(12315..13034)
                     /locus_tag="PLF90_01400"
     CDS             complement(12315..13034)
                     /locus_tag="PLF90_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK55972.1"
                     /translation="MWRNKKVSVIFPTYNEKDSIKQAIEDFFASGFVDEIIVVNNNAV
                     EGTDEEVRKTKARLIYEKKQGYGFAIQKGLEEATGDLLIISEPDGTFEGKDVIKLLAY
                     SDDFDYVLGTRTTKELIWEGANMNFFLKWGNWAVAKFMEFLYNTTTLTDMGCTMRLIK
                     RHLYEKIKNYFTVGREHFGPEMTLLVIKTGTKFIEIPVNYKPRVGKSSVTGSMRKAFF
                     LGMKMIGLILQYKFKRIKFKQ"
     gene            complement(13037..13720)
                     /locus_tag="PLF90_01405"
     CDS             complement(13037..13720)
                     /locus_tag="PLF90_01405"
                     /inference="COORDINATES: protein motif:HMM:NF017001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOK55973.1"
                     /translation="MKKNVFDEVAEIYEKIFPEHIFNYYLNKRINIILKLKLDKKAKI
                     LDVGCGTGTLLYHLKNYGYDVWGIDNSKRMVEIAEEKVPGKIIKEDMLNIPFPSETFD
                     LVFSIVSLHHLGKIEKVKKAVREMIRITKNGGMILIWEHNPLNPYWYFLMKKVPQDIG
                     EEKLIPLSIFINEFKKNKIKILKVFRCGLVPDFAPKWSLFFLDYLEKGLQKVFPLLLA
                     HNVIIGKKE"
     gene            complement(13701..14903)
                     /locus_tag="PLF90_01410"
     CDS             complement(13701..14903)
                     /locus_tag="PLF90_01410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOK55974.1"
                     /translation="MDNKKILYLFLFALILRIAFILTLDNSVDVWGDWWDELGWKIAS
                     GKGYWVDNPYFPDGPKFYAWRSPGFPLFLALIYKIFGHNYLTAKIGLAVLSSLTVILI
                     FFLGKTLIDEKKGILASIIYSIYPPSIFWTGYLAPETITTFFLTLSIFLILKSEKENL
                     KILNLILGGLSFGYLCITRPTFLLIIPFVFLIFFWKYKIKFFKKSLPFLLSIIIFPFL
                     WGLRNFIILKHFVVTSTEGGIVFFIANNEYSLSSPSGFYHSENVDEFKGLSEVEIDKL
                     LYKKTFDFIQKNPKIFLKLLFDRFFRFWRFYPHTISGPGEAYKKIHQIISFITETPII
                     ILGFLGIFISFREFKSLVLIYSVIFLYSGISIFIRTTIRYRFPIMGFLILFALYFLEK
                     CKYEKKCI"
     gene            14940..16337
                     /locus_tag="PLF90_01415"
     CDS             14940..16337
                     /locus_tag="PLF90_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55975.1"
                     /translation="MEKNKILKKILTYFNILYFFVLIFYDFPVLSPMFIDSGFYAYTA
                     QRIKEGKILYKDVWEAKFPAIYYIYTFLFTFLPESRWTLFLSDVILTFLTLFVINLIF
                     KNFSLDKYYLFTLPFLLTIYRVYPSYSGGNLNEHYFIFFFLLTYYLLTKKESSKLNSF
                     FTGITFLFSCMFKQSFILFMIVLAFFYRKKIYENFISFLTGLFLPLLFFVYVILKGYP
                     ESIDCTIFYPLYWSKKESKTFLEFILNLKTYLKNSPLVQLFILFLIVLFQKEKRLKME
                     IIIFVFISLLIIFKTPASHPHYGLIFFIPVVLALIYLLNNLQHKKIIIFTLIFLTLIP
                     AKYIYLKIKDSLKALKIIFIDKDFRCASSPVAFIIINNLKDGETFIMNAPDPTVYFLT
                     RTKSPIRFINFDYYIDEYYKEELKKEIRENPPDYIYWDRPISELERIFSLTKNEYYLE
                     KIYNNLYKFKLKRKF"
     gene            16337..17317
                     /locus_tag="PLF90_01420"
     CDS             16337..17317
                     /locus_tag="PLF90_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK55976.1"
                     /translation="MGPKYSFGFSDHLLIEIGNINFYQLHFEAKAIVKAYEKLKDIAE
                     ELEVKPPIPKMAGFCYPHIASLGAHIEFPADGEPKPFPLIKNIENIDKLKEPEDYLSA
                     PLIQKKLKTLSELKKLVPDTPNTIGHLYEGPITTAVLLMGQDFFLLVYDNPEKAHKLL
                     NFAVESALNYAKTILNHFGDDIKEGPKGIPDDFAGILSPELFSEFVLPYWNKLYEGLK
                     ATERHLHSELLKEEHLKFLKEVKIDVFDPSADQYLNGEILSKKCPCRFTLKIQSWEIF
                     NLSEEELEKLYIKLAGYKPECIGFSMERLVDLPKIKRLLKIARELEKDCL"
     gene            17314..17751
                     /locus_tag="PLF90_01425"
     CDS             17314..17751
                     /locus_tag="PLF90_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55977.1"
                     /translation="MKNFLKKLRVNSILTLLSIIYFFITSFPHNPTYNQMFIDSGIYA
                     YIGQQINNGKILYKEVWDYKFPAIYYIYAFLFKIFTDTRWTLYFTDIFSSIIMLILLF
                     LILKKFKIAEFFWIVSFLFITAYRIYTTFSGGNLTEHFFIFLP"
     assembly_gap    17922..18021
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18241..18852
                     /locus_tag="PLF90_01430"
     CDS             18241..18852
                     /locus_tag="PLF90_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55978.1"
                     /translation="MFLIPVCLVKDNLKFLFLSLFVSLIFIYFFTFALYSHYILLVNI
                     PLIIGAIILIKNYPKKLVIFSLLVCFLLPLNLIRKRFYHFNRSFYKFVILKDHRINVH
                     PLVFTVIKYVKPKETIFMIPDYCEVYFITKTESPWKFCGIGTDIKEYFKNELRKLIRE
                     KPPDYFYLEISIKDFEDIFQLKPDEYQLIHLEKNLFKFKMLSF"
     gene            complement(18849..20021)
                     /locus_tag="PLF90_01435"
     CDS             complement(18849..20021)
                     /locus_tag="PLF90_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK55979.1"
                     /translation="MSKFILEKKIDFEKHNEEVKKLWEEFKSENPSRVPVTFSMNSRM
                     ILLNSELNKWKYTWKDFFENPDVRWEVELNFQKWVRFNVCQDSFMGYPEKEWNGINVY
                     YLNCDEAMYFGCPVVFPENDMPFILPILKEDKKKLYKMKLPDVFENPVYKIALEHYYY
                     LEEKRKKIDFEGIPVGKTGIFGLGTDGPLTVACNLRGASEIMIDFYDDPQYIHDLLSF
                     ITDETIRRIKKVSEFLGISYPQQGWGFADDSIELLSTDMYKKFVMPYHQKLLNEFSKG
                     GPNSIHICGRVQRHLKTLKEELNIKTFDLGFPVDFGKTREELGEDVLLIGNISPVLLK
                     NGPVEKIREAVKNLCESGVMKGKKFILHDGNNCAPETPVLHFEVMYEAGKEFGRYY"
     gene            complement(20002..21180)
                     /locus_tag="PLF90_01440"
     CDS             complement(20002..21180)
                     /locus_tag="PLF90_01440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK55980.1"
                     /translation="MIKKSGITGLDRTKFPQLNKIKIAYVIGTLKVGGAEKQLYLLIK
                     NLNKEKFEPFLIALRGGRMEEDFKKITNLYILNKRFKFDILILFNLINILRKEKPDIL
                     HTFMFTSNTWGRLAGILSGIPLIIASERSADLWKKWYHILIDKILLRFTDKIICNSEE
                     VKKIYIQKIDGEKEKFEVIYNGIEIEKYEKLQEKIELKKNFGIKNEKIILTGGRLSFE
                     KNLDTFLYIANDIKKEIKEVKFIIVGEGDEKENLFKLTEKLDLKKDVIFTGYREDLPD
                     LIKISDIVVLISLWEGMPNLVIEGMLCKKPVICTEIGGGKEIIEDGKNGFLVNPKNKK
                     EIKEKILLLLKNDMICKTIGEAGYNYAKKKFLLEKMVENYENLYLKFLKRGENEQIYS
                     "
     gene            complement(21122..22306)
                     /locus_tag="PLF90_01445"
     CDS             complement(21122..22306)
                     /locus_tag="PLF90_01445"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOK55981.1"
                     /translation="MKRLVLIFFIIHLFLFSQNTSKDFINVIENLKSQDPMKRVYAAY
                     KIRNMQGDLSESIPYLLNILEDERIIYDKTLGKTTPSDEAKKTIIKIGSVSLPYIFEK
                     YSNEESSKLLKIKLIEILGEIKDKSSQVFIEKCINDRDIEIKEKVIEILSKDKDEVNF
                     LINNSKQDEYIKIKVIRCFGSNKVKESIPFLYEYLKDKNWEIRKYAIWALGEIKEDIE
                     IEKLIPLIKDKEFLVRKEVAETFEKIKNPLSIPQLIELLDDSNWMVRMASVKALGALK
                     DIRGFEPVLNMLNDKQIEVKIEAIKALANFKDKRATVSLISNLNDRYHLLIREYSAYA
                     LGEIGDKRAVYSLINLLKSENYELKRIAREALRKITGVDFGYDKEKWYNWAGQNKISS
                     IK"
     gene            complement(22322..24211)
                     /gene="asnB"
                     /locus_tag="PLF90_01450"
     CDS             complement(22322..24211)
                     /gene="asnB"
                     /locus_tag="PLF90_01450"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOK55982.1"
                     /translation="MCAICGIVKNKGIVEIEEIKRMANVMNYRGPDEEGYFFENNIGL
                     GHKRLSIIDLKTGKQPIYNEDESLILICNGEIYNFVELRKKLEEKGHIFKTNSDNEVI
                     IHLYEEKKEKCLSNLRGMFAFAIWDRKNKVLFLARDRLGKKPLVYSMKNGNIYFSSEL
                     KGILQLTEIKKNVDFEALDDFFTYQAIPSPKTIFKNIKKLSPAHYLIWKDGEIKIEKY
                     WQIDFTKKIYFKDENDYKELLWEKLKESTKIRLISDVPLGAFLSGGIDSSTIVGIMSE
                     FLTTPVKTFSIGFDVESFSEIKYAKIVAKKFGTEHNEFIVKPDIIEILPKLVWYYNEP
                     FGDSSMIPTYYVANQTKNYVKVALNGDGGDENFAGYPRYYQTKVLEKLHKISKSTGLL
                     NKINKNLILKIYNKFPYKFPVRILKWLQETDDYGFCYAYTRRLTSFSPEWKDKLYSTF
                     FKNEIKNYNSFTITENLWSRTENLDLLEKMIFCDFNLYLPEVLLVKMDIATMANYLEG
                     RSPFLDTEFIELIASFPPELKLKGRISKYILKEKLKNFLPDEILYRKKMGFGVPLGKW
                     FRDDLKNYLKEHLLSENFRKGDLFNNKEVEKIINEHISNKADHSSRLFLLLIFELWRK
                     VYNVF"
     gene            complement(24342..24860)
                     /locus_tag="PLF90_01455"
     CDS             complement(24342..24860)
                     /locus_tag="PLF90_01455"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOK55983.1"
                     /translation="MERLINQEKFEIEVLEYLNSGKFFDKLVFVGGTMLRVCYGLNRF
                     SSDLDFWIIKKIDIEKYFMNLENHLKKRYFIKDCVIKLHTILFEIHSPYYKKNLKIEI
                     RKEIKRIKYEKTIAFSQYSDIQVLVNTLYLKDTLRFKIESFLDRGEIRDCFDIEFLGK
                     KGNISAYRKKCC"
     gene            complement(24848..25435)
                     /locus_tag="PLF90_01460"
     CDS             complement(24848..25435)
                     /locus_tag="PLF90_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55984.1"
                     /translation="MGYEKIRVLKEKLYFTVEDFTETLGIKKDSAKVEVGRYIKKGYI
                     LRLKRNFFILREKWENLSLEEIFKIVNIIQVPSYISLMTALSYYEITTQIQQDLFESI
                     SLKRSFKKNIDSVIFNYYKIKKKYYKNFVRRENFFIAGKEKAFLDIIYLYSFGKYKFD
                     ISSIDFNKLDFKFLKKEIKNYPVRVQKKFYEIWKG"
     gene            complement(25925..26161)
                     /locus_tag="PLF90_01465"
     CDS             complement(25925..26161)
                     /locus_tag="PLF90_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK55985.1"
                     /translation="MVKDLIKLKPLKTWKNYYKQRGMSPWYDIIDWVGGYPFEVAKPE
                     KIFNFFYKRNFELIKLKTCGGGHGCNEFVFIKKH"
     gene            complement(26267..26719)
                     /locus_tag="PLF90_01470"
     CDS             complement(26267..26719)
                     /locus_tag="PLF90_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOK55986.1"
                     /translation="MKKFKFGKNWKNFLKVLNEKRIKEAEISLKELTGLNNLKEKTFV
                     DIGCGSGIFSLAARNLGASVYSFDCDEEAIECAKILKNKFYKDDEIWKIEKGDILNRE
                     YVESLKKFDIIYIWGVVHHTGNMWRAMENLLYFVKNDSYLIISVYNDQ"
     gene            complement(26716..27831)
                     /locus_tag="PLF90_01475"
     CDS             complement(26716..27831)
                     /locus_tag="PLF90_01475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOK55987.1"
                     /translation="MLIIIRDFFSIGGAENFAREFTVNLKKKNVEVKVITSRNSLFKK
                     REKFFNNIKIIQLYSPKKRIIGTIIYCFSLFLYLIFHCREYDIIQSFFLKHSSFVSVI
                     AGKLLKKKVFCRMECSGIFGDLNTLKKVPFSSIYLKVFKKSDGIIVLSNEMKKELESA
                     GFKRKKIFLIPNCVDIRKFKPSENKSIVKEKLGFKDEKIIIFVGRLTKQKGIDYLLEA
                     FKDINIPEKFLIIIGDGELKKYLVNKVEKLNIKEKVLFLGKIENVTQYLQIADVFVLP
                     SISEGLPISLLEAMACGLPVVVSEVGGNIDIVENKINGYLIEPRNISKIKNAIEELLK
                     DEKKMKLVSEKNRKKIEENYSYEIIIEKYLKLYLEGK"
     gene            complement(27840..28916)
                     /locus_tag="PLF90_01480"
     CDS             complement(27840..28916)
                     /locus_tag="PLF90_01480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK55988.1"
                     /translation="MENEEIFSIITVSLNQGKYIEETIKSVIFQEGDFYLEYLILDGG
                     STDNTLEILEKYKKGFENKEIKINCKGLEFRYISEKDEGPTNALNKGFKLTKGEIIGI
                     LNSDDIYPTGILKKVWNTFKKNEKIDIVYGDVKFIDASGNFVKIKKNKRNIKEKDFIY
                     ENALIQPEVFLKRKVIEKVGIFDENLKFANDYEFWIRCLKNNLKFKYIPYTLAIFRKR
                     YDARSSSSNLFIFVETLKVQKKYFGKTEKLLKNIGIYSAMYAYETKNSFENSFEMLEK
                     NLSKNEIYLNKREIKKAKSYGYLKFSIYKIFENKKEGIKSYLKGFKNNPFIFFTKNNL
                     IFIVRLILFHKNIYFGIKKFLKFF"
     gene            complement(28906..29838)
                     /locus_tag="PLF90_01485"
     CDS             complement(28906..29838)
                     /locus_tag="PLF90_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOK55989.1"
                     /translation="MDKNSSIYIAGHTGLIGSAITRILKQKGYKNLILKKRDELDLGI
                     QKDVERFFEKERPEYVFICAGRTGGIKANIEKGADFIYENSIINLNLIHFSHIFNVKK
                     LLYIGCSCMYPVNCPQPMKEEYLLTGKIEKTNEPYAIAKIMGLKMCQAYNEQYKTEFL
                     TVIGGNVYGSGEKQFDEGAHVIPALIKKFYIAKKENLKEIEIWGTGNAKRDFIYVDDI
                     ANACIFLMENYNGKEIINIGTGRGTSIKEIVEVLKEISGFKGKIIFNEKMPEGAGIRI
                     LDIEKIKKLGWVPEYNIYEGLKLTYKWFTEKYGK"
     gene            complement(29831..31033)
                     /locus_tag="PLF90_01490"
     CDS             complement(29831..31033)
                     /locus_tag="PLF90_01490"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOK55990.1"
                     /translation="MYKRKIPFGTIVITEKAKKIIYELLETKRVSQGKYVREFEKRFA
                     DIWGVKYGVAVSSGTDAIALALSVLYDFGAKRGDEVILPALTFIGTANAVLMAGFKPV
                     FVDVELETLNINPEKIEEKITEKTKAMLPVHLMGKPVNMEKICEITKKYNLFLIEDSA
                     EAHGSEYKGKNVGTFGDMGCFSTYVAHIISTIEGGIIITDNEEYAEILRSLRAHGRAC
                     KCDVCVLNTSSGYCKKRFKYGRDIRFVFERVGFSSKMNEIEAAVGIGNLEIYEEIIEK
                     RRKNFFKLKEIFKEFEDYFYTIDEAKEEKLSPHAFPFILKEKCKFKRDELVFYLNENG
                     IDTRDLFSSIPTQTPGYKFLGYKEGEFPISEYIGNNGIHIGVHQDIDDEDIEYVGEVI
                     KSFLEING"
     gene            complement(31026..>32021)
                     /locus_tag="PLF90_01495"
     CDS             complement(31026..>32021)
                     /locus_tag="PLF90_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK55991.1"
                     /translation="GIKGLWYRKNDEIVSGGYEEIITDLDSLPLPAWDLLPMEKYRAH
                     NWHCFKNIDERGNYAAIYTSFGCPFNCSYCNIKALYNNKPGIRYKSPEKVIEEIDELV
                     GKYNIKNLKIADELFVLNKERVNKICDLIIERGYKLNIWAYARIDTVDEEILKKLKKA
                     GFNWLCYGIESASERVRKGVSKKIQQDKIEKVIEMTKKAGIYILGNFIFGLPDDNYET
                     MNETLQLAKKLNCEYVNFYVAMAYPGSYLYEWALKNGIKLPDEWSGFAQLSEDTYPLP
                     TKYLKPEEVLKFRDDAFIDYYSNPEYLKMIEEKFGEKVVEHIKDMLKIKIKRKYV"
BASE COUNT        10641 a         5125 c         3373 g        12782 t
ORIGIN      
        1 ctctaccacc ttttttctgc actcaaatct atcaatttta ttaaattttc ccgcattttc
       61 attcataatt ccttcttcat tcattattaa aataaagtca agattatgtc tttgagcaat
      121 ttcaaaatcg ttggggtcat gagcaggagt tacttttact gctcctgtgc caaattcaat
      181 ttctacttta tcatctgcaa ttataggtat aattctctcc acaaaaggta attttacctt
      241 ttctcctata agatttttat aacgttcatc ctctggattt acggcaacag cagtatctcc
      301 aagcattgtc tcaggtcttg tagttgcaac ttcaataaat ccacctcgta aaagaggata
      361 ttttatgaaa tataaattac ttttttcatc tttatattca acttcttcat cagataaagc
      421 agtttcacat cttggacacc agtttattat ttttttccct ttataaatta aacccttttt
      481 atataatctt acaaacgctt ctttgacagc atttgataaa gtttcatcca ttgtaaattt
      541 tgttcttgtc cagtcacatg aagcaccaag tttttttaat tgaaaaataa ttcttgaacc
      601 atatttttcc ttccacttcc aaacttcttc tataaatttt tctcttccaa gttgtttcct
      661 tgtcagacct tttttagcca gttctttttc cacaacattc tgtgttgcta ttcctgcatg
      721 gtctgtgcca ggaacccaga gagaattata tccttccatt cttttccatc ttattaagat
      781 atcctgaatg gtattattga gagcatgacc catatgtaaa aatccggtaa tatttggagg
      841 aggaataact ataacatatt tttctttttt attgtcagtt ttaacataaa aataagaatt
      901 ttcctcccac attttatata ttttttcttc tatctcaagg ggattatact tactttccat
      961 tttacttttc tcctaaataa ataatttcac ttaattcatt tattaccata atatctgagt
     1021 tgtcaaaaat catatttttt attttttcgc atatattttc aatttctttt ttctcactat
     1081 ttacaaaaca caaaccaatt acagttcttt gccatttatc ttgatgttca agttctgcaa
     1141 tagatacatt aaattttgat tttatcttat tttggagtga ttgaatcacc cttctcttat
     1201 cttttaaact ggaacttccc ggtataataa tttcaaattt taaagttcct ataaacattg
     1261 aaaaaataat tttaacatta ttttaaatta ttttccagat ttttgtagac cttttggtat
     1321 actgttttaa gaggaatttg tttttcttca gcaatttttt tacaatcctc atactccgga
     1381 gatatattta caatccggtt atccatatat gaaattttta cctttatttt tccaaattca
     1441 gtatcaattt cttttatttc cctttctaaa ataactctgt tttcttttct acatcttata
     1501 ccaatggtag tagtttcttc aaataagata ttaattacct cattaaattt ttcttcagga
     1561 agtataattt ctattttaaa aacaggtctg tttttcttac ctatgccaga aaatatagat
     1621 acatcaaaaa ctccctttct ataaagtttt tcaaataaat ttcctattaa aactggattc
     1681 atatcatcaa tatttgtttc tatgatcata acactttcat tttcctgttt tatttctccc
     1741 attaagaatt tcagcatatt tggtaatggt tcttctcttg ttcctgcacc atatccaact
     1801 ttactgatat aaaaatctga aacaatagga gtaaaactgg aaatcccttt gataattgca
     1861 gcacctgttg gagttgtaat ttcctgtgga acatttttga gcactgttgg tattcctttt
     1921 aaaatttcta ttgttgcagg agctatattt gaaacaccta ctgcacttga aaaaactttt
     1981 tctatttcaa gtaatttaat cccaagtacg acacttgtta tttcaataat tgcatcaatt
     2041 tgacctacct gatggaaatg gagtttttct ttttcttctt tatgaacaat actttctcct
     2101 tcagcaagaa tatcaaaaat cttcattatt ttttctttta taattataga aaatgaactt
     2161 ttttcaataa ttttaagaat atctttatat gaagtttctt cttcccattt ttcaactgga
     2221 ataactaaaa attgtgttcc ttctatagca tgtccaaatt ttgtctttct ttttactttt
     2281 ttcgtttcta ttctgtaatt tttaagaggg attttggtaa gttctttttt taaaatttca
     2341 atatcaagac ctgcatctat taaacttcca gcaagcatat ctcctgcaca tccatcaaaa
     2401 acttcaaaat atccaatttt tttcattctt tttttcttgg cattacctgt gaaatcgcat
     2461 ttttaacaac atcaattttt gtctcttctt taaatcttaa agttacaata tcttctttaa
     2521 tcttttctac ctcaccaata agtcctccaa cagtaataac tttgtctcct tctttcaatt
     2581 catcaagcat ttttttatga agttttgctt tcttttgttg gggtaaaata agtagaaaat
     2641 aaaaaatgaa aaaaattaaa attaaaggta ataatgctcc taaaggtgat gcttgaggtg
     2701 gagttgcttg tgccattgtt aaattcattt tgcctccttt taattatttt tgcttttact
     2761 ttcttttttt cctatagatt ttaaatattt ctctgtatac ttttttatgt tttttgaaac
     2821 aaggtaattg attgttcctt caggatatgt tccatctttt cttttttcac ctgcaggaat
     2881 acctgttaaa atttctattc cttcatcaat ggtttttact gcccatatat gaaataaacc
     2941 tttctcaacc gcctctatta cttcatcatt taaaataaga ttttttaaat ttctttccgg
     3001 tataattaca ccctgttttc ccgttaatcc ttttgattta caggtgtaat aaaatccttc
     3061 tattttttca tttatacctc caaccggttg aatttctcct ttttgattta cagagccagt
     3121 aacagcaatt ccctgtttta taggaatatt tgcaagagcg gaaagtaaca catatatttc
     3181 tgttgatgat gcactatccc cttcaatttc ttcatatagt tgttcaaaac atatacttgc
     3241 tgagaaagaa ataacttctt cctgtccata tttttcgtta atatatgtat ttaatattaa
     3301 aaatccttta ttatgaattt ttcctgcaag tttaacctct ctatcaatat taattactcc
     3361 tccttttcct gtaaatacat tacaggttat ttttgatggt tttccaaaac tataatcacc
     3421 aagagaaata acagataacc catttatttg tcccaccttc tcaccatctg tttcaaccat
     3481 tattgttcct tcttttatta attcttcaat ccttttttct attaaatttg acctgtaaat
     3541 tttttcttca agtgcctttt ttacatgttt ttctcttaca attggagagt tatccatctc
     3601 cgcccagaaa tttgcttctc taataatatc ggcaatttct ataaatcttg tagttaattt
     3661 gtttctatct tctactattc ttgaaccata ttccattatt ttaccgactg caaacttatc
     3721 aagatgtttc aaattttctt ctttacatat tttacttata aaagaagcat aatttttata
     3781 accttcttca tttttttcca tgattgtact gaaatcagcc cttactttaa ataatttcct
     3841 gaaatcttct tcatatgtgt aaaggagata ataaaaaact ggatgtccaa ttaatataac
     3901 ctttatatct aaaggtatag gattgggtct taaagttgga gtattaatca atctgaactg
     3961 ttcatttaaa tcttctataa ttatttcatt attttttaat gctcttttca atgtttccca
     4021 tgaataataa tttttgaaaa catcaaggat atgtaaaatt aaataaccac catttgcttt
     4081 atgtaaagaa cctggtttta tcattgtaaa atctgttacc attgctccaa aatgtggtcg
     4141 gtattctatc cttccgcaaa gattataagc ggttggatta gtttctttaa ctacaggagc
     4201 tccttttgtc tgtgaattat caattaacac attaacagta tatctgtaaa gagatgattc
     4261 tctatcagga attttaagtc caggaagtaa ctcaatttct tttttttctt taaaatcatc
     4321 tatattctct atcatatcct tttcaacttc ttcaaggtat ttacagacat cactgaattt
     4381 tttatatttt tctttaagtt cctgaattct cggcgatatt gtaaattttg ctgtttcctt
     4441 ctcaagttga tttaactttt cctttgcttc tttttcaaaa tcccttattt ttctcaatgc
     4501 atcatttaat tcttcatata acatttcctg cttttttctt atttcagttt ttgcttcttc
     4561 tgttaatttc tccatttctt cttcttttat tggctgtcct tcaattaatg gtataaaaac
     4621 tattcctgaa ggtgtctgtt ttatctgaaa tccagaagtt tttgcagttt tttctatttg
     4681 atcaagaagt tggttccttt tgttctggaa ttcttttata atctggtttc tatgttcttc
     4741 atatatttca ctctcaaatg tttttgggag ttctactttt aattcattta caaggatttc
     4801 catatccttt ttaaattcat ttgctttccc aggaggaaac cttaaaacat ttggattatc
     4861 aggatttgta aaattataaa cataacacca atcatcaggg gttttccctt tttctgctat
     4921 tttttttact gcctgttcaa cagatgtagt tcttccactt cctgttatac cagtaacaaa
     4981 aatattatat cctttacttt ttatttttaa tccaaattca agtgcttttt ttgctctttc
     5041 ctgtccaata attccttcaa gaggcggaat ttcttcagta gtttcaaaat ttaaaaattt
     5101 aaggtcatat ttttctttta aatatttaac atccactatg tatttttcca tttttaagtt
     5161 cctttttagt taaataatct tacaatttca cacttataaa gtcaaatttt ggtgatttat
     5221 tagaggaaat aattgaaagc atcaaggttt attttaagaa ttttttcagg aagagtgatt
     5281 tttatcgctt ctacaaaact ctgttttttt aaaggtataa attttgatgc aaatccaagc
     5341 attagcgtat taattacttt tggtgaacca atattttctg ctatttttgt aaaattttca
     5401 atgtaaatat tttttgtttt tgattttaag aactcaataa ttttttcata ttcatattct
     5461 ttacccggaa ttgggattgg aaaattattt acaatcatta tcccatcttt ttttaaataa
     5521 aaagaacttc tgcatgcttc cattgtttca aatccgatta ttaaatcagc atatccaaca
     5581 ggtattttag gtccgaaatt acagtttaat ctaacatatg cctctaccga ccctcctctt
     5641 tgtgccatac catgaatttc agacccttta acactgattc cttcaataac tgcagcattt
     5701 ccaataagat ttgaacagag aataatgccc tgaccaccaa caccacaaat cagaatatca
     5761 aatttatctc tcatttttct tttttatagc accaaatgga catatttgtt cacaaatacc
     5821 gcaaccatta cagagattcc ctatttctat attatctttt accaaaataa ttcctgaaca
     5881 tccaaattca agacataatt tacatttttt acagatattt ttatcaatat aaaattcagt
     5941 ttttttaatt tttgaaatat ggatgcattc tcttttaaat attataactt ttactccttc
     6001 tttttctttt atttgtttta tcttttcaac agaatcttta attaaataag ggtcaacctc
     6061 tacaagattt ttaactccac atccttcaat aagttttttt aaatcaattt caactgtttt
     6121 atctccttta gcattaattc ctgttccagg atgtggttga tatcctgtca ttgccgtggt
     6181 tctattatca agtattgcta taacctgatt tgaatgattg taaacagcat ttattaatcc
     6241 aggaattcca gcatgaaaga aagttgaatc tcctataatt gagataatat attttttctc
     6301 aaatcttgaa attcctgttc cgatagatat tccacttccc atacatagac atgtatcaat
     6361 tgtaccaaat cttattccga gagtataaca acctatatct cccggaaaaa tagattcttt
     6421 tccaaaaact ttttttagaa tgtaaaaaga acctatatgg ggacatcccg gacataaagt
     6481 aggagttctt tctggtaagt taatatttac atatacattt tttctttttc ctgaaaaaga
     6541 atattcaata tcttcaacag agagttctcc ttcttttttt acatcaccac tcatttttcc
     6601 ttttattttt atttcaggat tatatttttt tgaacataga tatacaaatt cttcaactac
     6661 agggtcacct tcttcaaaaa caaaaacatt ttttaatcct ttcaaaaatt ttcctaattt
     6721 tttttcagga ataggataag agccaatttt cagaactgga atagattttt ttttgaggtt
     6781 taaaaattct tttacataaa tatatgataa accagaagca ataattccag tggaaccttc
     6841 accattgtca attttgttta tttttaactt ttctatgtct ttttttataa cttcctgttt
     6901 tttatttaaa tttattatta cctttctaac attggaaggt accattacaa attgagaagg
     6961 attttttata aaagttgatg ttgagttttt ttcttctctt ttacctatta atacatccgc
     7021 tttcccatga gataatcttg taacacttct tattaaaaca gggagtccgt atttttcaga
     7081 aaaatcaaaa gaaaaaatta ctgtatcttt tgcttcctga attgatgatg gttcaaaaca
     7141 aactaatttt gcaaactgtg catatcttct ggagtcctgt tcattttgag atgaatgagc
     7201 aaaagggtca tctgcaacaa ttattaaaaa tccaccaaca attcctgtat atgcagatgt
     7261 aagtaatgag tcagaagcaa catttaatcc aacatgtttc ataatggcac tgcttcttct
     7321 ccctgttaaa cttacaccat aagcattctc aaaagcaact ttttcattta cactccattc
     7381 tatatataca tcattttttg aaattttaag aagtgcctca gtaatttcag aagaaggagt
     7441 tccaggataa gcaaaagatg catttactcc accttcatat aatccataag caattgcttc
     7501 atttccaagt aaatattttt tttccatagt taaaaattat aacatttttg cggacaatta
     7561 ttaaataaat aactcatcag aaaatttttg gctggaaaat gtaaacctca actaaataat
     7621 aatactcttt tacacctttt aatctgctta aaagtttttc attataaata tatttttttt
     7681 catacaattt ttcatactta ttttcaaaaa attctcttat ttcttttgaa atttcaggat
     7741 tatttatgaa atcaagaata agaaaaaatc ttgatttctt tttttctctg ttaaaaattt
     7801 taattacttc atcaatatca tttttatcat taatcctaaa tactttattt tttccactat
     7861 aataccacat cagaggggtt aatatttctc tgtcatactt ttctgttgta aggataatat
     7921 ctccatcttt ctctattttc tgaatttcct tgaatatttc tctgaaaaga attaacttgc
     7981 ttgtgtcatg atattgcgag atgttgtctg tataatagaa aattaaagat ggaagaagaa
     8041 ataaaaagtt caaataaata agtaagtttt tataattaaa attatgcatt cctatggaaa
     8101 agagtaaata ccagaagaag gaaataaaaa ttaagttttg gggaacagtt gaaggaaaaa
     8161 aaacgaaaaa aagcgggaaa ataagtgata tactcaacag gactttaatt tctttttgaa
     8221 gttttaaaag atttttcaca ccaattgtaa aaatgattga ataagtgatg aaagcaggaa
     8281 ttaaaatttt gaagttaaaa ggacttaaag tttctccaaa gaaaaaataa aaaagagtgt
     8341 aaggcaattt tagtaatgct ggaaatggtc ttctcacacc gagagtaaaa ttcatctgat
     8401 aggggatttt cagaagccat ggaatataaa ttagaaaaat taaaaaaaat aaaagaaagg
     8461 attttctatt taactttttt aaaatcaaaa gagttagtat ttgaattagt ataacaataa
     8521 aaccaaagtt atgtgtgtag agaattaaaa tattagagag acaaaaaata ggtagaaatt
     8581 tcttattttc tgaattcaaa atttcaataa aaaagataaa agacagaatt gtcaaaaaag
     8641 tgagaagtga atatgattta attagttgag accataaaat aaaaggagga ctgagagaga
     8701 caaataaaga gagaaagaga cctatttttt cattataaaa ttttttgcca aggatataac
     8761 tgtaaggtat acataataac ccaaataaca tgggtaaaat tcttaaaata atttcattct
     8821 caccaaactt taaccagaaa tgaaccagca aataaaaaaa tggtggatga gcatcattat
     8881 atgttatata atcaatcatc cattttattg agtgtttacc aagagaaata gtgagtaatt
     8941 catcacacca taaggagtgt tttccaagat gtaaaactct gaaaaaaaat ccaagaaaaa
     9001 aaatcacaaa taaaaaaaat ttaatttttc tattcatttt ttaaaatgac aactacactt
     9061 ttttctataa aaaacttcca tacgtttaaa atagaaaatt ttctatttat aaattctttc
     9121 attataaaaa ctttttctac tttaaaattc ttcatctttg caatttcaaa gattgacttt
     9181 ttattaaaat gataaatctg ggcaggaata tgtaaaattg atttactttt tggtgtaaat
     9241 gagaaaagtt tagccagaaa aaacatataa tttgagtgta agggagtttt tataattaaa
     9301 atacccccct ctttcaataa ttttttactt ttttcaaaat aaaagagagg gttttttaca
     9361 tgagcaataa catccatcat tgttattata tcaaaagatt tttcaggaaa attaaaatta
     9421 tcaaaaactt ttaagttatt ttctttgcag aaattaattg caaattttga aatttcgtat
     9481 cctataactt catatttttt ttcttcaaac attttcatta atatgcccac tccacatcct
     9541 atatccagaa ttttaccttt ttcaggaata aaatttttca atttcagata caacctttca
     9601 aaatattttt tcctatcatc ataataagga aggtaccatt tttcaaaata ttttttgtca
     9661 taaatttttt caatgttttc aggaaatgga tataaaaaag aaatttcaca ttttttacaa
     9721 ttatatatct ggtaattttt aatttttaat tttaaattag tatttttttc acataaagga
     9781 catttaacca ttttaattaa taaatctgta ttttattaaa gtccatatac ctataatacc
     9841 gtctttcaaa cggatttttt taccctcttt tatagttctt ggattataat gaattgggac
     9901 ttcataaatt ttgtaccctt tttttcttac ttttgctgtt acttccggac agaattcaaa
     9961 acccttgcat tttagattta ttgattttat aacatctttt ttaaacattt tataacaggt
    10021 gggttcatct gtaatttgtg cattatagag taaatttgcc aatattgaaa gaattctgcc
    10081 tcctaaataa aaaaataaag aagatttttt accttttccc agaattcttg aaccatatac
    10141 aacctctgct tttttttctt caattaattt aatcatttta agccagtcca ttggttcata
    10201 ttctaaatca gcatcctgaa ttacaaccac atctcctgta atatactcaa gtcctttcct
    10261 tattgccatt cctttccctt catttttttc tttaaagatt atcttgatat ttttatcttt
    10321 taagttttta agaatttctc ttgttttatc attacttcca tcatccacaa ttataatttc
    10381 tttttcaaaa ggtacttttt tcactttttc tactactttc attattgttt tttcttcatt
    10441 aaaaacagga ataatcactg ataatttcat tttatcctct ttgaataaat aaatttttaa
    10501 ccattaaaat tatataagaa aaaccgtaag ttgataaaat aatataaaat ggttcaatgg
    10561 gtactctgta tcttatggaa gccagaaaaa ttatatggaa aactgtaaaa agtattatca
    10621 aactatgaaa aaattgtgct tttttatttt ttaatgaaat aaaaaaaccg attataaaaa
    10681 aaggcatcat aattccaaag gaaaaaacac ttataaaacg atataaaatt tttctttcaa
    10741 attcaggagc atttggaatt atattccaga atcttctgaa tttattttgg attaaccaga
    10801 gaaatctttc tggatttttt cttataacat caaaagtcat tctgtataaa attttatctc
    10861 ttgttatttc atctacttca agaatacctt caggaaaatg ggaacatgga cctccttctg
    10921 aatatggatt atttccttcc cagaaaaccc aaccacccat agttgttcca ggaataaacc
    10981 cgtgaaatat tctgtaattt cttaaaatcc atggtgaaag tgttaaacaa aaaccgattg
    11041 taataaaaaa gattttttta atttttttta caaaattttc ttttaaaaaa aatatgtgta
    11101 ttagagaaac tggaaaataa agttgaaaaa ttggtcttgt aagtccagaa attcccataa
    11161 aaattcctgt taaaaaagat ttttttccat ttctttcaac aatttttatc agaaaaaaaa
    11221 tactaagaac tgtaaaaaaa ataaaaaaag tttctgtcag aatgaaacca ttataaaaaa
    11281 tgaaataagg ataaaaagca gaaatccatc cactccataa tccaattttt ttgttaaaag
    11341 tttctttccc gatattgtaa attagatata ttgtaaatgt tgatataagg gtctgtatta
    11401 ttcttattcc tgtaagattt atttttaaaa gagtaaaaat actcaataaa aagggataaa
    11461 aaggtggtct gaaactttta agattttcac caactattaa tcctttccca gcaataaaat
    11521 tttgaaccat tttaaaatat tcatactcat catcaaaata aaattgattt tttaaggtta
    11581 aaacgaaaat taaacgtaat aaaaaaccta taaccagaag ataaattaaa tttttccttt
    11641 cagatactct ttccatatct ttaatttgta tataaaaaaa ttgttgtgtc cataaattcc
    11701 aattcttttc attgtaaaat cagtccattt aattttctgt cccctttttg gtgtaacaaa
    11761 cgcgccttta tatccagcaa gttttacaaa atctattatt ttttcattaa attctccata
    11821 aggatagcag aaaaatttaa tttctttacc tgttttatct tcaattattt tttttgaact
    11881 tattatttct tcttttgctg ttttttctga aatctgtgta agatgtggat gtgtcagagt
    11941 atgagagcca atttccactc cttcctttat catttcattt atttctttcc aatttaaaaa
    12001 tctatctttt tctttatcct tatatcttga aaaaatttta tttgtaccag tataatcaac
    12061 gatcaaaaaa attaaaggaa aaattctgaa actttttaaa atgtcaaatg caaaccagaa
    12121 attatcagcg aaaccatcgt caaaagtaat aacgattttt ctctttaaaa tttttttatt
    12181 ttcttttaaa tattcttcca caggaacaat caaaaatttt ttagagatta aatactcaat
    12241 ctgtttttta aaatattcag gttttactgt caaagcatca ttataccagg gattaattct
    12301 atgataagca agtatcattg tttaaatttt atcctcttaa atttatattg taaaatcaga
    12361 cctatcatct tcataccaag aaaaaatgcc tttctcatac ttccagttac tgatgattta
    12421 ccaactcttg gtttataatt aacaggaatt tcaataaatt ttgtccctgt ttttattaca
    12481 agaagtgtca tttcaggacc aaaatgttcc cttccaacag taaaatagtt ctttattttc
    12541 tcataaagat gcctttttat aagacgcata gtacaaccca tatctgttaa tgttgttgta
    12601 ttgtaaagga attccataaa ttttgcaaca gcccagttac cccatttcaa gaaaaaattc
    12661 atatttgcac cttcccatat aagttctttt gtagtccttg ttccaagtac ataatcaaaa
    12721 tcatcactgt aagcaagaag ttttataaca tcctttcctt caaaagttcc atcaggttca
    12781 gatattataa gtaaatcacc tgttgcttct tcaagtcctt tttgaattgc aaaaccatat
    12841 ccctgttttt tctcataaat aagacgtgct tttgtttttc ttacctcttc atctgtccct
    12901 tcaactgcat tattattcac aactattatc tcatcaacaa atccagaagc aaaaaaatct
    12961 tctattgcct gctttatgga atctttctca ttataagtag gaaatataac agaaactttt
    13021 ttattcctcc acataattat tccttttttc ctattataac attatgagca agtaaaagag
    13081 gaaatacttt ttgtaatccc ttttctaagt aatctaaaaa aaataaactc cattttggag
    13141 caaaatccgg aactaaacca catctgaaaa cttttaagat tttaatttta ttttttttaa
    13201 attcattgat aaaaatactc agaggaatta atttttcttc tcctatatcc tgaggaactt
    13261 ttttcatcag aaaataccaa tatggattta atggattatg ttcccaaatt aaaatcattc
    13321 ctccattttt tgttattctt atcatttcac ggacagcttt ttttactttc tcaatttttc
    13381 ccaggtgatg taaagaaaca attgaaaaaa ccaaatcaaa agtttcggaa ggaaaaggaa
    13441 tatttaacat atcttctttt attattttac cggggacttt ttcttctgca atttcaacca
    13501 ttctttttga attatcaatt ccccagacat cataaccata attttttaaa tgataaagca
    13561 aagttcctgt cccacaaccc acatcaagaa tttttgcttt tttatcaagt tttaatttta
    13621 atataatatt tattctttta ttcaaataat aattgaaaat atgctctggg aaaatttttt
    13681 cgtatatctc ggctacttca tcaaatacat tttttttcat atttacactt ttctaaaaaa
    13741 tataaagcaa ataaaataag gaaacccatt ataggaaaac gatatcttat tgtagttctt
    13801 ataaatattg aaattccact ataaagaaat atcactgaat aaataagtac caaactttta
    13861 aattctctaa atgaaataaa aatccccaaa aatcctaaaa ttattattgg agtttcagta
    13921 atgaaactta ttatttgatg aattttttta tatgcttctc caggtcctga aattgtatgt
    13981 ggataaaaac gccagaatct aaaaaatcta tcaaataaaa gttttaaaaa aattttagga
    14041 tttttttgga taaagtcaaa ggttttttta taaagaagtt tatcgatttc aacttcagac
    14101 aaacctttaa attcatcaac attttcagaa tgatagaatc cagatgggct gcttaaagaa
    14161 tattcattgt ttgcaataaa aaaaacaatt cctccttctg tggatgtaac aacgaaatgt
    14221 ttaagaatta taaagtttct tagcccccat aaaaaaggga aaataattat acttaataga
    14281 aaaggtaagg attttttaaa aaatttaatt ttatatttcc agaagaagat taaaaacaca
    14341 aaagggataa ttagtaaaaa ggtaggtctt gttatacata aatatccaaa acttaatcct
    14401 ccaagaatta aatttaaaat ttttaaattt tctttctcac ttttaagtat taaaaaaata
    14461 cttaatgtta agaagaaagt tgttatagtt tcaggtgcaa ggtagccagt ccagaaaata
    14521 gaaggtggat aaattgaata aataatagat gccaaaattc cttttttttc atcaataaga
    14581 gttttcccta agaaaaatat taaaataact gttaatgaac ttaaaactgc aagaccgatt
    14641 ttggctgtaa gataattatg tccaaaaatt ttatatatta atgctaaaaa taagggaaat
    14701 cccggacttc tccatgcata aaatttaggg ccatcaggaa aatatggatt atcaacccag
    14761 tacccttttc cagaagcgat tttccatccg agttcatccc accagtcacc ccatacatca
    14821 acagaattat caagtgttaa aataaatgca attcttaaga ttaaagcaaa taaaaacaaa
    14881 tataaaattt ttttattgtc catttttttg tatataatta taaattaaaa gaacaaaaaa
    14941 tggaaaaaaa taaaatacta aaaaaaattt tgacctattt caatattctt tacttttttg
    15001 tccttatatt ttacgatttt ccagttctat ctcctatgtt tattgactct ggtttttatg
    15061 catatactgc tcaaaggatt aaggaaggta aaattttata taaagatgtc tgggaggcaa
    15121 aatttcctgc aatatattat atttatactt ttcttttcac ttttcttcct gaaagtagat
    15181 ggacattgtt tttatctgat gtaattctaa ctttcctgac tttatttgtg ataaatttaa
    15241 tttttaaaaa tttttctctg gataaatatt atctttttac tttgcctttt ttattaacaa
    15301 tttatagagt ttatccttca tacagtggtg gaaatttaaa tgaacactat tttattttct
    15361 tttttctttt aacttattac ttattaacaa aaaaggagtc gtcaaaactc aattcttttt
    15421 ttacagggat tacatttctt ttttcctgta tgtttaaaca gagttttatt ctttttatga
    15481 tagttttagc atttttttac agaaaaaaaa tttatgaaaa ttttatttct tttttaacag
    15541 gattatttct tcctttattg ttttttgtat atgttatttt aaaaggatat cctgagagta
    15601 ttgattgtac aatcttctat ccattatact ggtcaaaaaa ggaatcaaaa acatttcttg
    15661 agtttatttt aaatttaaag acctatttaa aaaatagtcc acttgtacag ctgtttatat
    15721 tgtttttaat tgtgcttttt caaaaagaga aaagattaaa aatggaaata ataatttttg
    15781 tttttatttc tctacttatt atttttaaaa cacctgcttc ccatccacat tatggactta
    15841 tcttttttat tccagtagtt cttgctttaa tatacttatt aaacaattta cagcacaaaa
    15901 aaattattat ttttactctt atctttctca ctttaatacc cgcaaaatac atatacttaa
    15961 aaataaaaga ttctttaaaa gcattaaaaa ttatttttat agataaagat ttccgatgcg
    16021 cttcttctcc tgttgccttt ataataatta acaatttaaa agacggagaa actttcatta
    16081 tgaatgcacc ggaccctact gtttactttt taacaagaac aaaaagtccc attagattta
    16141 ttaattttga ttattacata gatgaatatt ataaagagga actaaaaaaa gaaataaggg
    16201 aaaatccacc agattacatt tattgggaca gaccaatttc agaacttgaa aggattttca
    16261 gtttaacaaa aaatgaatac tatcttgaaa aaatatataa taatctatat aagtttaaac
    16321 tgaaaaggaa attttaatgg gaccaaaata cagttttgga ttttcagacc atcttttaat
    16381 tgaaataggt aatattaact tttatcagtt acattttgaa gcaaaagcaa ttgtgaaagc
    16441 atatgaaaaa ctaaaagata tagcagaaga acttgaggta aaaccaccta ttccaaaaat
    16501 ggctggattc tgttatcctc atattgcttc actcggtgct catattgaat ttccagcaga
    16561 tggagaaccc aaaccatttc cattaataaa aaacattgag aatatagata aattaaaaga
    16621 accagaagat tatttaagtg ctcctttaat acagaaaaag ttgaagacat tatcggaatt
    16681 aaaaaaactt gttccagata ccccaaatac aataggacat ctttacgaag gaccgataac
    16741 aaccgctgtt ttacttatgg gacaggattt ttttctgctt gtatatgata atcctgagaa
    16801 agcacataaa cttttaaatt ttgcagttga aagtgcttta aattatgcaa aaacgatatt
    16861 aaatcatttt ggagatgata taaaagaagg accaaaagga ataccagatg attttgcagg
    16921 tattttatct ccagaattat tttctgaatt tgttttacct tactggaata aattatatga
    16981 aggattgaaa gcaactgaaa gacatctgca cagtgaacta ttgaaagaag agcatttgaa
    17041 gtttttaaaa gaagttaaaa ttgatgtttt tgacccatca gcagaccagt atttaaatgg
    17101 agaaatacta tcaaaaaaat gtccatgcag atttacttta aaaattcaat cctgggaaat
    17161 ttttaattta tctgaagaag aactggaaaa attatatata aaacttgcag gttataaacc
    17221 agaatgtata ggtttttcaa tggaaagatt ggttgactta cccaaaataa aaagattact
    17281 taaaatagca agagaactgg aaaaagactg tttatgaaaa attttttaaa aaaattaaga
    17341 gtcaattcta tactgacact tttgagtatt atatattttt ttattacttc ttttccacac
    17401 aatcctacat ataatcagat gtttatagat agtggaattt atgcatatat aggacagcaa
    17461 ataaataatg gaaaaatttt gtataaagaa gtttgggatt ataaatttcc agcaatttat
    17521 tatatttatg cttttctttt taaaattttt actgatacaa gatggacatt atattttaca
    17581 gatatttttt caagtattat catgttaatt ttgctttttc ttatactgaa aaaatttaaa
    17641 attgcagaat ttttttggat tgtttctttt ttatttataa ctgcttatag aatttatact
    17701 acatttagtg gcggtaacct tacagaacat ttttttattt ttttaccata atttctttat
    17761 ttattttatt tgaaaaacca acaaaaatag gggattttat tctgggaaat tgtttaatat
    17821 ggatgttgat gtttaaacaa ccttattctc tgattgtttt ggtaattttt ttatttttta
    17881 aagatagaat tttgaaaggt gagaaaagtt tttcatttac gnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18001 nnnnnnnnnn nnnnnnnnnn nttttttatt ttttaaagat agaattttga aaggtgagaa
    18061 aagtttttca tttacgtatt cattaaaggt tttccagaaa gttttgaaac atttactttt
    18121 ccattttatt tagcaaaaac ttcaaatatc tcatcaaaaa acacacttat aaatttttta
    18181 aatgatataa attacagatt tagaattttt ttatttacag gaccaggaaa acaattattg
    18241 atgtttttaa taccggtatg tctggtaaaa gataacttaa aatttttatt tttaagttta
    18301 ttcgtaagtt taatattcat ttatttcttt acttttgctt tatattccca ctatattctt
    18361 ctagtaaata tcccattaat cataggagca ataattttaa taaagaatta tcctaaaaaa
    18421 ctggttattt tttccctctt agtttgtttt cttttacctt taaatttgat aagaaaaaga
    18481 ttttaccatt ttaatagaag tttttataaa tttgtaattt taaaagacca cagaataaat
    18541 gtacatcctc ttgtttttac agtaattaag tatgtaaagc caaaagagac aatctttatg
    18601 attcctgatt attgtgaagt ttactttata acaaaaactg aaagtccttg gaaattttgt
    18661 ggaattggta ctgacattaa agaatatttt aaaaatgaat taagaaaatt gataagagaa
    18721 aaaccaccgg actatttcta tcttgaaatt tcaattaaag attttgaaga catttttcaa
    18781 cttaagccag atgagtatca attaatacat ttagagaaaa atttatttaa atttaaaatg
    18841 ttgtcttttt aataatacct gccaaattct ttacctgctt catacattac ttcaaaatgt
    18901 aaaacaggtg tttcaggagc gcaattattt ccatcatgta atatgaattt ttttcctttc
    18961 ataacccctg attcacaaag attttttact gcctctctaa ttttttcaac aggtccattt
    19021 ttcaaaagta caggtgaaat atttcctatt aaaagcacat cttctccaag ttcttctctt
    19081 gtttttccaa aatcaacagg aaatccaagg tcaaatgttt ttatatttag ttcttccttc
    19141 aatgttttta aatgtctctg aactcttcca cagatatgta tactatttgg tcctcctttt
    19201 gaaaactcat ttaataattt ttgatgatat ggcataacaa attttttata catatcagtt
    19261 gaaagtaatt caatactgtc atctgcgaac ccccagccct gctgtggata agatatacca
    19321 agaaattcac ttactttttt aattcttcta attgtttcat ctgttataaa agaaagtaaa
    19381 tcatgaatat attgcgggtc atcataaaaa tcaatcatta tttcactggc accccttaaa
    19441 ttacaagcaa ctgttaaagg tccatcagtt cctaatccaa aaattcctgt tttcccaaca
    19501 ggaatacctt caaaatcaat tttttttctt ttttcttcaa ggtaataata gtgttcaagg
    19561 gcaattttat aaacagggtt ttcaaagaca tccgggagtt tcattttata aagtttcttt
    19621 ttatcttctt ttaaaattgg taaaataaag ggcatatcat tttcagggaa aactacagga
    19681 catccaaaat acattgcttc atcacagttt aaataataaa catttatacc attccattct
    19741 ttttctggat atcccataaa tgaatcctgg cagacattga atcttaccca tttttgaaaa
    19801 tttagttcta cttcccatct aacatcaggg ttttcaaaaa aatctttcca tgtatacttc
    19861 catttattca attcagaatt aagtaaaatc attcttgaat tcatggaaaa agttacagga
    19921 actcttgatg gattttcact tttaaattct tcccacagtt tttttacttc ttcattatgt
    19981 ttttcaaaat caattttttt ctcaagaata aatttgctca ttttctcccc tttttaaaaa
    20041 ttttagatat aaattttcat aattttcaac cattttttca agtaaaaatt ttttctttgc
    20101 ataattataa cctgcttctc caatagtttt acaaatcata tcatttttaa gcaacaataa
    20161 gattttttct tttatttctt ttttattttt aggattcacc agaaacccat tctttccatc
    20221 ttctattatt tcttttcctc cacctatttc agtacatata accggttttt tgcacaacat
    20281 cccttcaatt acaagatttg gcataccttc ccataaggaa ataagaacca ctatatctga
    20341 aatttttatc aaatcaggta ggtcttctct atagccggta aatataacat ccttttttaa
    20401 atcaagtttt tcagtcaatt taaatagatt ttctttttca tcaccctccc ctactattat
    20461 gaattttact tctttaattt cttttttgat gtcatttgcg atatataaaa atgtatcaag
    20521 gtttttttca aatgataatc ttccgccagt caaaatgatt ttttcatttt taataccaaa
    20581 atttttcttt aattcaattt tttcctgtaa tttttcatat ttttcaattt ctataccatt
    20641 atatataacc tcaaattttt ctttttctcc atcaattttc tgaatataaa ttttttttac
    20701 ttcttcagaa ttacatataa ttttatcagt gaatcttaag agaattttat caattaaaat
    20761 atgataccat tttttccaca agtcagcact tctctctgat gcaattatta aagggattcc
    20821 tgataaaata cctgctaacc ttccccatgt atttgatgta aacataaaag tatgcaaaat
    20881 atcaggtttt tcttttctca gtatgtttat cagattaaaa agtattaaaa tatcaaactt
    20941 aaatcttttg ttaagtatat aaagatttgt aattttctta aaatcttctt ccattcttcc
    21001 acctcttaga gcaattaaaa agggttcaaa tttttcttta ttgagatttt taattaataa
    21061 ataaagttgt ttttccgctc ccccgacttt taaagttccg attacatatg cgattttaat
    21121 cttatttaat tgaggaaatt ttgttctgtc cagcccagtt ataccacttt tctttatcat
    21181 aaccaaaatc aacccctgtt atttttctta atgcttctct tgcaatcctt ttcaattcat
    21241 aattttcact tttcaataaa tttattaaag aatagaccgc tctcttatca ccaatttctc
    21301 caagtgcata agcagaatac tctcttataa gtaaatgata tctgtcattt aaattagaaa
    21361 taagtgaaac tgttgctctt ttatctttaa agttagcgag tgcttttatt gcctcaattt
    21421 ttacctcaat ttgtttgtca ttcagcatat taagtacagg ttcaaaacct ctaatgtctt
    21481 ttaatgcccc aagtgccttt acagatgcca ttcttaccat ccagtttgaa tcatcaagta
    21541 attcaatcag ttgaggaatt gatagaggat tttttatttt ctcaaatgtt tctgcaactt
    21601 cttttctaac aagaaattct ttatccttta taagtggaat tagtttttca atttcaatat
    21661 cttcttttat ttctccaaga gcccatattg cgtattttct tatctcccag tttttatcct
    21721 ttaaatattc ataaagaaat ggtattgatt cttttacttt attactgcca aaacatctta
    21781 ttactttaat ttttatatac tcatcctgct ttgaattatt tattaaaaaa ttcacttcgt
    21841 ctttatcttt tgaaagaatt tcaataactt tttcttttat ctcaatatct ctgtcattta
    21901 tacatttttc aataaatact tgtgaacttt tgtcttttat ttctccaaga atctcaataa
    21961 gttttatttt aagtaattta gaactttctt catttgagta tttctcaaag atataaggaa
    22021 gtgaaacaga accaattttt ataattgttt tttttgcttc atctgaagga gtagttttgc
    22081 ccagtgtttt atcgtaaatt attctctcat cttcaaggat atttaagaga taaggtatac
    22141 tttcagataa atcaccttgc atatttctta ttttataagc agcatatact cttttcatag
    22201 ggtcttgtga tttcaggttt tcaattacat ttataaaatc ctttgaagta ttctgagaaa
    22261 ataaaaataa gtgtataatg aaaaaaatta aaaccagtcg tttcatacta cctccttttt
    22321 tttaaaaaac attataaact tttctccaca attcaaaaat aagtaaaaga aataatcttg
    22381 aagaatgatc tgctttattt gaaatatgct catttataat tttctcaact tctttattat
    22441 tgaataaatc accttttctg aaattctctg acaacaaatg ttcttttaaa tagtttttaa
    22501 ggtcatctct aaaccattta ccaagaggca ctccaaagcc catttttttc ctgtataata
    22561 tttcatctgg gagaaaattt ttcaattttt ctttcagaat atatttactt attctccctt
    22621 ttaatttcaa ttctggtgga aaagaagcaa tcaattctat aaattctgta tcaagaaatg
    22681 gacttctccc ttcaagataa ttcgccatag ttgctatatc catttttaca agaagaactt
    22741 caggaagata tagattaaaa tcacagaaaa tcattttttc aagtaaatca agattttcag
    22801 ttctggacca taaattttca gtgattgtaa aagaattata atttttaatt tcatttttaa
    22861 aaaacgtact gtataactta tccttccatt caggagaaaa agaagttaaa cgtcttgtat
    22921 aggcatagca gaatccataa tcatctgttt cctgtaacca cttcaaaatt ctaactggaa
    22981 atttatatgg gaatttatta tagattttca gtatcaaatt tttatttatt ttattcaaaa
    23041 ggcctgtact ttttgatatt ttatgtaatt tttcaagaac ttttgtctgg taatatcttg
    23101 gataacctgc aaaattttca tctccaccat ctccatttaa ggcaactttt acataatttt
    23161 ttgtttgatt tgcgacataa tatgttggaa tcattgatga atcaccaaat ggttcgttgt
    23221 aataccagac aagttttggc aaaatttcta taatatcagg ttttacaata aattcattat
    23281 gttctgtccc aaactttttt gcaacaattt ttgcatattt aatttcagaa aaactttcaa
    23341 catcaaaacc aattgaaaaa gttttaaccg gtgtggtcag aaattcactc ataataccta
    23401 caatagtaga agaatcaata cctcctgaaa gaaatgcacc aagagggaca tcagaaatca
    23461 atcttatttt tgtggattct tttaattttt cccatagtaa ttccttgtag tcattttcat
    23521 ctttaaaata tatttttttt gtaaaatcaa tctgccagta tttttcaatt tttatttcac
    23581 cgtctttcca tatcaagtaa tgtgcaggtg aaagtttttt aatatttttg aaaattgttt
    23641 ttggagaagg aattgcctga tatgtaaaaa aatcgtcaag tgcttcaaaa tcaacatttt
    23701 tttttatttc agttaactgt aaaatcccct ttaattcaga agaaaaataa atgtttccat
    23761 ttttcattga ataaacaagt ggtttttttc caagtcggtc tcttgcaaga aataaaactt
    23821 tattctttct atcccatata gcaaatgcaa acatgcctct taaattagac aaacattttt
    23881 cttttttttc ttcgtaaaga tgaataataa cttcattatc tgaatttgtt ttaaaaatat
    23941 gacctttttc ttcaagtttc tttcttaatt caacaaaatt atatatctct ccattacata
    24001 ttaaaattaa actttcatcc tcattataaa tgggctgttt tcctgttttt aaatcaataa
    24061 tacttaacct tttatgtcct aaaccaatat tattttcaaa aaaatatcct tcttcatcag
    24121 gtcctctgta attcataaca tttgccatcc tttttatttc ttctatctca actattcctt
    24181 tattttttac tattccacat attgcacaca ttttaagaat tgatgatttt gttcaattca
    24241 cctttcaaaa actcaaaaat ttttttaaaa taaaaatctc tttctctctt atctataatt
    24301 gaaccaataa acttaaattt tccatctttt tgataagttt ttcagcaaca tttttttcta
    24361 tatgcagaaa tattcccttt tttaccaaga aactctatat caaaacaatc tctaatctct
    24421 cctctatcta aaaaactttc tattttaaat cgtaaggtgt cttttaaata aagagtgtta
    24481 actaaaacct gtatatctga atattgagaa aaagcaattg ttttttcata tttaattctt
    24541 tttatttctt ttcttatttc aattttaaga ttttttttgt aataaggaga atgaatttca
    24601 aaaagtatgg tatgtaattt aataacacaa tctttaataa aatacctttt tttcaaatga
    24661 ttttctaaat tcataaaata tttctctata tcaatttttt taataatcca gaaatcaagg
    24721 tcactggaaa atctgtttaa tccataacat accctgagca tagtacctcc aacaaaaaca
    24781 agtttgtcaa aaaattttcc gctatttaaa tattcaagga cttcaatttc aaatttttcc
    24841 tggttaatta acctttccat atttcataaa attttttttg aactctgaca ggataatttt
    24901 ttatttcttt tttcaaaaat ttaaaatcaa gtttattaaa atcaatggat gaaatatcaa
    24961 atttatattt accaaatgag taaagataaa taatgtccag aaaggctttt tcttttcccg
    25021 cgataaaaaa attttctctt ctgacaaaat tcttgtaata ttttttttta attttataat
    25081 agttaaaaat cacgctatct atattttttt taaaagacct ttttaaagag atagattcaa
    25141 aaagatcttg ttgaatttga gttgtaattt cataataact taaagcagtc atcaatgaaa
    25201 tgtaagatgg aacttgtata atattaacaa ttttaaaaat ttcttctaaa gatagatttt
    25261 cccatttttc tctcaagata aaaaaattcc tttttaatct caatatataa ccttttttta
    25321 tgtatctgcc tacttctact tttgccgaat ctttttttat cccgagtgtt tctgtaaaat
    25381 cttcaactgt aaaatacagt ttttccttta aaacccttat tttttcatat cccattttta
    25441 agtacaataa attaacaatt ttgttaactt tctatatttt aaaataatca aatataatat
    25501 gcaaatttct tctttggaag ctgtttcata aaatactttc gtaaatgtat actgaaccgc
    25561 gtttcctttt ttattttgtt atattttgtt tctactgtca actacggttc tttcttaaag
    25621 gagtttaacc ccctttaaaa accccaagag gtatcaactc ccaaaatggg tagttcacca
    25681 tctcgctacc cgtgctaatc agtgttcctt attcgttgca ccagcatccc atcaatcttt
    25741 gagggtttta aggtaattat aacttgtttt tggtataaag cgatctgaaa aaacacttgg
    25801 aatttttcaa attccagtgt tttaaacctg tggttgagca agtatttacg tactttctta
    25861 ttacacatat tgcacacatt ttgaatataa taagttaaca attttgtcaa tttttctata
    25921 tcctttaatg tttttttata aaaacaaatt cattacaacc atgaccaccg ccacaggttt
    25981 taagtttaat caattcaaaa tttcttttat aaaaaaaatt aaatattttt tctggctttg
    26041 ctacttcaaa agggtaccct ccaacccaat caattatatc ataccatgga gacatccctc
    26101 tttgtttgta ataatttttc catgttttga gtggttttag ttttattaaa tcttttacca
    26161 ttgttggttc ccataatctt aaaaaaacaa taaaaataat aaaaggctta aaaaaataat
    26221 gtgaattata aattttcttt atatttttcc agattttact tgcaaatcat tggtcattgt
    26281 agacagaaat aattaaataa ctatcatttt tcacaaaata taaaaggttt tccattgccc
    26341 tccacatatt tccagtatga tgaactactc cccaaatata aataatatca aatttcttga
    26401 gagattcaac atattcccta ttcaatatat cgcctttttc aattttccag atttcatcat
    26461 ctttataaaa tttgtttttt agtatcttcg cacattcaat tgcctcttca tcacagtcaa
    26521 aagaataaac acttgctcct aaatttctcg ctgctaaact aaaaattcca cttccacatc
    26581 caatatcaac aaaagttttt tctttcaagt tatttaatcc tgttaattcc ttcagagaaa
    26641 tttccgcttc ttttattctt ttttcattta atacttttaa aaaattcttc cagttttttc
    26701 caaatttaaa tttcttcatt ttccttccaa atacaatttt aaatatttct ctataattat
    26761 ttcataagaa taattctcct caattttctt tctgtttttt tcacttacta acttcatttt
    26821 tttctcatct tttaacaact cttctattgc atttttaatt ttactaatat ttctcggctc
    26881 tattaaatat ccatttattt tattttcaac tatgtcaata ttaccaccca cttctgaaac
    26941 aactacagga agtccacatg ccatagcttc aagtaatgat ataggaagtc cttcagaaat
    27001 agaaggtaaa acaaaaacat ctgctatttg taaatattga gtaacatttt ctatttttcc
    27061 caaaaataaa accttttctt tgatatttag tttttcaacc ttattcacta aatatttttt
    27121 caattctcca tcccctataa taatcaaaaa tttttcaggt atatttatat ctttaaatgc
    27181 ttccaagaga taatcaatac ccttttgttt tgtcaatctt ccaacaaata ttataatttt
    27241 ttcatctttg aatccaagtt tttctttaac aatactttta ttttctgatg gtttaaattt
    27301 ccttatatct acacaatttg gaataagaaa aatttttttt cttttaaatc cagcactttc
    27361 tagttctttt ttcatttcat tacttaaaac aattatcccg tcactctttt taaaaacttt
    27421 taaataaata cttgaaaatg gtactttttt taaagtgttt aaatctccaa aaattcccga
    27481 acattccatt ctgcaaaaaa cttttttctt taataatttt ccagcgataa cagaaacaaa
    27541 agaactatgt tttaagaaaa atgattgaat aatatcatat tctctacagt gaaaaatgag
    27601 atataaaaat aaagaaaaac aataaatgat tgtaccaata attctttttt ttggagagta
    27661 aagttgaata attttaatat tattaaaaaa tttttccctt tttttaaata aagaatttct
    27721 tgatgttata acctttactt ctacattttt cttttttaaa tttacagtaa actctcttgc
    27781 aaaattttct gctccaccta ttgaaaaaaa atctcttata attattaaca ctctcatttt
    27841 caaaaaaatt taagaaattt tttaatgccg aaataaatat ttttatggaa aagaattaac
    27901 cttacaatga aaattaaatt gttttttgta aaaaatataa aaggattgtt tttaaatcct
    27961 tttagataac tttttattcc ttctttttta ttttcaaaaa ttttatatat agaaaatttt
    28021 aaataaccat aagattttgc ttttttaatt tctcttttgt ttaagtaaat ttcgtttttt
    28081 gataaatttt tttccagcat ttcaaatgaa ttttcaaaag aatttttggt ttcataagca
    28141 tacatagcag aataaattcc aatatttttt aataattttt cagtttttcc gaaatatttt
    28201 ttctggactt ttaaagtttc aacaaaaata aataaatttg aacttgaact tctcgcatca
    28261 tatcttttcc tgaaaattgc aagagtataa ggtatgtatt tgaattttaa attatttttc
    28321 aaacatctta tccagaactc atagtcattt gcaaatttta aattttcatc aaaaattcca
    28381 actttttcta ttacttttct tttcaaaaaa acctccggtt gaatcagagc attctcataa
    28441 ataaaatctt tttctttaat atttctttta ttttttttta tttttacaaa atttcctgat
    28501 gcatcaataa atttaacatc tccataaacg atgtctatct tttcgttttt tttaaaggta
    28561 ttccatacct tttttagaat cccggtcgga taaatatcat cagaattaag tattcctata
    28621 atttcccctt ttgttaattt aaaaccttta tttagagcat tcgttggtcc ttcatccttt
    28681 tctgaaatat atctaaattc aagtccttta cagtttattt taatttcttt attttcaaac
    28741 cctttcttat atttttcaag tatttcaagg gtattatctg tagaaccgcc atctaaaatg
    28801 agatattcaa gatagaaatc tccttcctga aaaattacac tttttattgt ttcttctata
    28861 tattttccct gatttaagga gacggttatg atagaaaaaa tttcttcatt ttccatattt
    28921 ttccgtaaac catttatatg tcaattttaa accttcatat atgttatatt ccggtaccca
    28981 tcctaatttt tttattttct caatgtctaa aattctaata ccagcacctt ctggcatttt
    29041 ttcattaaat attattttcc ccttaaaacc actgatttct tttaaaactt caacaatttc
    29101 ttttatactt gtacctcttc cagtcccaat atttataatc tctttaccat tgtaattttc
    29161 cattaaaaaa atacaggcat ttgctatatc atcaacatat ataaaatctc tttttgcatt
    29221 tcctgttccc catatctcaa tttcctttaa gttttctttt tttgcaatat aaaatttttt
    29281 aatcaaagca ggtataacat gtgctccttc atcaaattgt ttttctcctg atccataaac
    29341 atttcctcct ataactgtta aaaattcagt tttatattgt tcattatatg cctgacacat
    29401 tttaagtccc attatttttg caattgcata aggttcattt gttttttcaa tttttcctgt
    29461 taataaatat tcctctttca ttggctgggg acaattaacc ggatacatac aggaacatcc
    29521 aatatagaga agttttttaa cattaaaaat atgagaaaaa tgaataagat tgagattgat
    29581 tattgagttt tcataaatga agtcggcacc cttttcaata tttgctttta ttcctcctgt
    29641 tcttcctgca catataaaaa catattcagg tctttctttt tcaaaaaatc tttcaacatc
    29701 tttttgaatt cctaaatcaa gttcatctct tttttttaaa attaaatttt tatatccttt
    29761 ttgtttcaaa attcgtgtta ttgctgaacc aataaggccg gtatgacctg ctatgtaaat
    29821 agaagaattt ttatccattt atttccagaa aactctttat tacttctcca acatattcaa
    29881 tatcttcatc atctatatcc tgatgaactc ctatatggat tccgttattt ccaatatatt
    29941 cagaaatggg aaattcacct tctttatatc ccaaaaattt atatccaggt gtctgagtgg
    30001 gtattgaaga aaaaaggtct cttgtatcaa tgccattttc atttaaataa aaaacaagtt
    30061 catctctttt aaacttacat ttttccttta aaataaaagg gaaagcatgg ggacttaatt
    30121 tttcttcttt tgcctcatca attgtataga aataatcttc aaattcctta aaaatttctt
    30181 tcagtttaaa aaagtttttt cttctttttt caattatttc ttcatatatt tcaaggtttc
    30241 caatgcctac cgccgcttca atttcattca tttttgagga aaagccaact ctttcaaata
    30301 caaatcttat atccctacca tatttaaatc tttttttgca atatccggaa gaagtattta
    30361 aaacacaaac atcacattta cacgctcttc catgtgctct taaagacctc aatatttcag
    30421 catattcttc attatctgtt atgataattc ctccttcaat tgttgaaatt atatgggcaa
    30481 catatgtact gaagcaaccc atatctccaa aagttccaac atttttacct ttatattctg
    30541 aaccgtgtgc ttctgctgaa tcttcaatta aaaatagatt atattttttt gtaatttcac
    30601 atattttttc catatttaca ggttttccca taagatgtac gggtaacatt gcttttgttt
    30661 tttctgttat tttttcttca attttttctg gatttatatt taatgtttca agttctacat
    30721 ccacaaatac aggtttaaaa ccagccatca aaactgcatt tgcagttcca ataaaagtta
    30781 aagcaggaag aattacttca tcacctcttt ttgcaccaaa atcataaaga actgaaagag
    30841 caagagcaat tgcatctgtt ccactgctta cagcaactcc atatttaact ccccaaatat
    30901 ccgcaaacct tttttcaaat tctctaacat attttccctg agatactctt tttgtttcaa
    30961 gtaattcata tattatcttt tttgcttttt ctgttattac tattgtgccg aaaggtattt
    31021 ttcttttata catatttcct cttaattttt atttttaaca tatcttttat atgttcaaca
    31081 actttttctc caaacttttc ttctatcatt ttcaaatatt caggattact gtaatagtca
    31141 ataaaagcat catctctaaa tttcaaaacc tcctccggtt ttaaatactt tgtaggtaaa
    31201 ggatatgtat cttcagaaag ttgtgcaaaa ccactccatt catctggcag ttttatacca
    31261 ttttttaaag cccattcgta taaataagaa cccggataag ccatagcaac ataaaaattc
    31321 acatattcac aatttaattt ttttgcaagt tgaagtgttt cattcatagt ttcataatta
    31381 tcgtcaggaa gtccaaaaat gaaattcccg agtatataaa ttcctgcttt ttttgtcatt
    31441 tcaattactt tttcaatttt gtcctgctgt atttttttag aaacaccctt tcttactctt
    31501 tcacttgctg attcaattcc ataacatagc caattaaaac ctgctttttt taactttttc
    31561 aatatttctt catcaactgt atctattctt gcataagccc atatattaag tttatatccc
    31621 ctttctataa tcaaatcaca tattttattt accctttctt tatttaaaac aaaaagttca
    31681 tcagcaattt ttaaattttt aatattatat ttacccacaa gttcatctat ctcttcaatg
    31741 accttttccg gcgatttata tcttattcct ggtttattat tataaagtgc ttttatgtta
    31801 caataagaac aattaaatgg acatccaaaa cttgtataaa ttgcagcata attacctctt
    31861 tcatcaatat ttttaaaaca atgccagtta tgcgctctat atttttccat aggtaataaa
    31921 tcccatgcag gtaaaggtaa agaatcaagg tctgttatta tttcctcata tccaccactt
    31981 actatttcat cattctttct ataccacaga ccttttattc c
//
LOCUS       DAOSAT010000009        30979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_1083_length_30979_cov_5.518012, whole genome shotgun sequence.
ACCESSION   DAOSAT010000009 DAOSAT010000000
VERSION     DAOSAT010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30979
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1083_length_30979_cov_5.518012"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLF90_01500"
     CDS             <1..283
                     /locus_tag="PLF90_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOK55992.1"
                     /translation="ESQNYYIQHEKGTDPFSWIKIIPFMDEETQKKYPIDHAGKQETS
                     LMMVFYPSGVKMKKYSKEKWYSKEAKKANKEYGKKAKKEIIKRLKEVLK"
     gene            390..1952
                     /locus_tag="PLF90_01505"
     CDS             390..1952
                     /locus_tag="PLF90_01505"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HOK55993.1"
                     /translation="MEKIKKLIFVIFFQVIILNSQNSLNLYEQENWSTLESEIDRYVF
                     SKLEKLNIKHAFPCSDEVFIRRIYLDTIGMLPEVEEVIDFLNDKNPDKRKVLIDKLLE
                     KKEFAEYWSMKWCDILRIKAEFPINLWPNGVQAYHKWILNSLVENKPYDKFVRELLTS
                     SGSNFRVPPVNFYRAVGKRDPYSIASCVSLTFMGVRFEKLPDDIKTGMSKFFSKVSYK
                     KTLEWKEEIVYFNPENSETIEAIFPDGNKIKIPPDKDPRTVFADWLIRPDNPYFSKNI
                     VNRIWSWIFGRGIIHEPDDIGPDNPPAIPELLNYLENQLIKSNYDLKHIYKIIFNSRV
                     YQQSFIPRDNNPEGEKYFAYYLPRRLEAEVLLDIICKFSGSGIEYVSQIPEPYTFIPS
                     SQRNILLADGSITGPFLELFGRPPRDTGLESERNNKINEAQMRYLLNSSELHKKIQSS
                     NYIRQLMREAKGDRKKIIENIYLSLLSRYPSKSEIELLEGYFQKQGISFNQAVEDIVW
                     ALINSKEFLYKH"
     gene            1963..3249
                     /locus_tag="PLF90_01510"
     CDS             1963..3249
                     /locus_tag="PLF90_01510"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOK55994.1"
                     /translation="MNKIFKIFMVIFIVFNFSQKSYSQSEIRPKAVIQIWLWGGPSHL
                     DTFDPKPNAGYDYTGPFKSIPTNVPGIEINELLVNLAKLADKYSIIRSMTHGINAHET
                     AAYLMQTGHSSEENLVYPSIGAVVSLFKGYDYGYKGILPPYIVLTTSQGRFSECGFLG
                     PKYKPFVTGGDPNRTPFAVEGIVLEGISEERKNARKELLYSIDILGKLIPWSKEFQWF
                     DLCREKAYEIISGEIAKVFDLSTEPEEVRNMYGRNTFGQSCLVARRLIEKGVVYVTIN
                     YRGWDTHNNHFQQMRQKLPELDMGLASLIKDLSDRGLLDSTIIWVSGEFGRTPKVQWE
                     PPWNGGRGHYGKCFSVLVAGGGFKGGCVVGKSDEKGEEVIDRPVYPKDLLGSIYYLLG
                     IDPEGKFPEKFGKDARILPPIEKGPKKGLLKEIMYE"
     gene            3242..5500
                     /locus_tag="PLF90_01515"
     CDS             3242..5500
                     /locus_tag="PLF90_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HOK55995.1"
                     /translation="MNKRSIFLIFLISCNFIFAQIMTNQPSIGYVYQAGGQRGTTLEI
                     IVGGQNIRGARNAYFSTDKIKVLQVNPLPSLNPQQKRLLAKNLREIITNMYRKEYRKP
                     EIVYEMDGVKLPKHPLLENLENKSFEELRFIAETFLGPFRTEQIKRSIQEKVMIKVEI
                     SPDTEPGIYELRVQSPLGLTNFLNFYVSEYKEVKEKEKIPYYTIFDPLKEVFDIPLII
                     NGQITPGDVDRFYFNAKKGQKLVIELKGREIIPFMADAVPGWFQGVLTLYDSSGREIA
                     YADDYYFNPDPVIFYEVKKDGEYMIEVRDALYRGREDFVYRLFIGEKPFITHIFPPGG
                     ERYEKTIVSIYGWNLPQKTVELDTEDIGIHRKSFDFNGIYSNQIYYMVDEIPEINERE
                     PNDSLKNAMQIIMPQIVNGTISKSGDIDIYKFKCSKGDKIVAEVYSRRLNFPLDSIIT
                     LMDSSGKVLISNDDYFDKSFDTITHHADSYIYYEIPDDGIYYFKITDVKGHGGDEYIY
                     RLRIEKSKPDFKIFIVPSAINITSNNTVPFYIYAVRKDGFDGEIEVSLKDCPEGLILS
                     GNKIPKGKDKICMTITADTNFNFLNKPVRIKIEGTVNINGKLIKREAIPADEMMQAFA
                     YYHLLPSSELLFYPGRSRFFKSQPISLNEKFIKIPSGGSVKIEGKISGYSENEKITLE
                     LKDPPDGITIEDLKIENDTVSFRIKAEPKIKKGFSDNLIIEVFSEPKEETSQKKQRSS
                     KGFLPALMFEII"
     gene            5511..6698
                     /locus_tag="PLF90_01520"
     CDS             5511..6698
                     /locus_tag="PLF90_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HOK55996.1"
                     /translation="MEKNKIEIKGIPVEIKYSSFKGKSGVEEFHFSFTPKEFGDFRTQ
                     LKWIYNAYTETLKEKGISEETSVFRRFFCSDLYNQVEYLREMDFSNPENISNPTCISW
                     ICQPPLPYSKISLWAYHIKDPENKLKKELKGKNTCCIIREDIIHYWTGGIFSIEKDSI
                     YEQTKNIIEKYVEFLNENNMKLSDNVIRTWFFLQNIDTDYRDFVVARREIYEKNGLTK
                     NTHYIVSTGVGGSYYDLKAKVILDAYSIYGIKTQQIKFLSAPDFLSPTYVYGVTFERG
                     VSISYSDRKHIIISGTASINNKGEIMYPKDINLQTERTLLNIEALLKNENATLNDMMI
                     FIVYVRDPADYYFVEKKINERFKNTPTIFTTASVCRPGWLVEIEGIAVIPSPPLPTFK
                     VDR"
     gene            6794..7438
                     /locus_tag="PLF90_01525"
     CDS             6794..7438
                     /locus_tag="PLF90_01525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012185508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOK55997.1"
                     /translation="MEFKIKNNQKILFIGDSITDCGRREQFRPLGNGYVKLFSDLVIA
                     NFPERKIEIVNKGISGNTILDLANRWEDDVIYQKCDWLSILIGINDIHRVLRKDEMWT
                     EFTPENYRKRYDYILKRTLEKLDCKIILIEPFYISTDTTDSFRGKVLKDLIEYIEIVE
                     EMSKKYETYHLKLHRIFQEYLKFFEPETFAPEPVHPNSTGHFVIANELIKLLSE"
     gene            complement(7430..9163)
                     /locus_tag="PLF90_01530"
     CDS             complement(7430..9163)
                     /locus_tag="PLF90_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOK55998.1"
                     /translation="MDIGKIIKVSGPLVQAEGMKTSKMFDVVRVGEEKLIGEIIRLEG
                     DIASIQVYEETEGLKVGDPVFGTGEPLRVELGPGLLQNIFDGIQRPLSQIEKTGDFII
                     RGVDIPPLDRSIKWDFKPVVKKGDYVESGDVIGIVDETPLIKHKIMVPYGVNGEIIEI
                     KEGSFTVEEEIGKVKTDKGEIPLYLMQKWPVRKPRPFKKRLNPDTPLVTGQRVIDTLF
                     PILKGGTACIPGPFGSGKTVVLHQIAKWADSEIVVYIGCGERGNEMADVLIEFPHLKD
                     PKSGRPLMERTCLIANTSNMPVAAREASIYTGVTIGEYFRDMGYSVALMADSTSRWAE
                     ALREISGRMEEMPGEEGYPAYLGTRVASFYERAGNVICLARDNKNGSLSLLGAVSPPG
                     GDLTDPVVQMTLRVVKVFWGLDDRLAYQRHFPAINWLTSYSLYERNIEKFVKEKIASD
                     FMEIRAEAISLLEKEAELLEIVRLVGMETLSLTDRLILEIARSIREDFLHQSAFDEMD
                     AYTTLKKQYLMLKTIILFYRVSLKAIEKGRTLEEILKNPVREEISRMKYVPEDKLEEI
                     IKLQEKISSLF"
     gene            complement(9165..9464)
                     /locus_tag="PLF90_01535"
     CDS             complement(9165..9464)
                     /locus_tag="PLF90_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOK55999.1"
                     /translation="MENRIIFIGDKLSIEFFSAFNIETFFANSVKEAEEILEKIDMKD
                     VICIFITEEVFDEDKFMKFLNMKKMVVIPGLKSREGKGVKIVDELIKKATGMKGD"
     gene            9641..10945
                     /locus_tag="PLF90_01540"
     CDS             9641..10945
                     /locus_tag="PLF90_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOK56000.1"
                     /translation="MRNKIFFVFLLLVSFLYCEKNLVIISPHWEGVKIEFTRAFCEWY
                     EKNYKEKVKIEWIDQGGTTDDLKYVESLFKKNPDGIGIDLFFGGGLSPYLTLKKEGLL
                     EKYKLPQNVLKKIPKYCAGIPNYDDNFYWYGVVLSGFGILYNKKALKFLNLPIANAWK
                     DLGDSRYFSLIGAADPRHSGSMHMMYEIILQTYGWEEGWKTILSIGGNTKNFSTSASE
                     VARETSIGEAVFSLCIDSYALAQIEVNGKENMGFIFPESQTVINPDCIGILKGAPNIE
                     IAKKFIDFLFTYEGQLLWIQKKGKKGGPKEYSLNRFPVMPEVYNEPGLEIDINPYRIQ
                     NTIKYDFQLAANRWALIDDMIGCFVIDCHSELKKAWHIISKTNNKNLKERFYEIPVDK
                     KIQRFLWENWKDPIFRNKYINEWLNFSLKKFRNIIVESKFLM"
     gene            10956..12014
                     /locus_tag="PLF90_01545"
     CDS             10956..12014
                     /locus_tag="PLF90_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK56001.1"
                     /translation="MDKEILTVENVDKSYNGKEVLKNINFSVYENEFFFILGPSGCGK
                     TTLLRIIAGFVEPDRGRIILNKKDVTKTPPNKRNIGFVFQNYALWPHMKVIDNIAYGL
                     RIKKYSEEFIKRRVKEIIEITKLKNVENLYPSQISGGQQQRVALARALVVNPQILLLD
                     EPLSNLDAKLRDELRREIKRIHKETGITMVYVTHDQKEAMTLGTTIAVMKEKEIVQIG
                     SPYEIYSNPENLFVASFIGDINSLKGKIVEKKGNILKIETDEGFFFAKIKKTINSENV
                     YLCFRPESITVEEKINTITGRLHDYEFYGDIVKINIITPKGNTFKIGIYADDFNKYKK
                     DENIVFSVKEDKIMVFEE"
     gene            12011..13675
                     /locus_tag="PLF90_01550"
     CDS             12011..13675
                     /locus_tag="PLF90_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOK56002.1"
                     /translation="MKRKKILLTLIFLFFLLFLVYPVSFILIQCFYNNGNFTLEILKI
                     AITNYVIRKSIINSFILGIIVVLTTSIIGIPLAFIFDRLNFKGKSILRILFLLPMIAS
                     PFVGSIGIKQFLSRFGSFNILLIKLGILKNPISWIGSGFLGIVILQTLHLYPIMFLNI
                     TASLNNYDISVEEASYNLGATGLQTFFKVIFPLILPGYFAASSIIFIWSITDLGTPLV
                     FEFSNLVSVKIFYTLKDINTNPVGYALVFIILLLTLLLFIFTKRYLEKTPYTTGKTAI
                     YVKEKNIVGRKLFFLYLFLFFILFLSLIPHFTLIFYSFSKKWFFSILPAEYTGEYYKV
                     VFAHRLTKTGIFNSLIYSSISTTIDIFLGFFIGYILARWKIKGKELLDILSMVPLVIP
                     GIVMAFGYVATFSNTILDPKNNPVFLIVLSYSLRRLPYLVRTTYSSFLQLDRGLEEAS
                     YSLGGDNIITFKKIIFPLVSPGLFAGGILAFAFSLMEVSCSLILAIREKFYPISKVIY
                     TLAGRVTDGPNTATSLGVLGMLITGICLFISTKIFSKKIGEFFRIG"
     gene            13736..13954
                     /locus_tag="PLF90_01555"
     CDS             13736..13954
                     /locus_tag="PLF90_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56003.1"
                     /translation="MMIDFNSPEWKERLKGKTPEEIAKIVEEYYERKYKKEYPVFPWK
                     ILLAIIILFILLIIILWGAIYYLKRVSF"
     gene            complement(13951..14811)
                     /locus_tag="PLF90_01560"
     CDS             complement(13951..14811)
                     /locus_tag="PLF90_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK56004.1"
                     /translation="MENKIIMHVNYMEQGQSIEEICEKAVKLGYDGVEFRRKMKNMDE
                     EEYLEKLAKAVEKSKLEYVLFGAPGPNLMIPEKDKREREIEEAVNFYRNASRYFNLTI
                     CNTMTGVLVNPSFSYFEFDKHGSNIAKEEHWEWAVSGFKILGDVAEELNFKFAFETHM
                     CYLHDTVESTKKLVDLIDKKSVGINLDYANIVCFKNPVSLMDAIEMCGEKLYYIHLKN
                     LFKIEGIEYKNYINCSLADGIINNREFLKILKIKNYTGFLCLEAPRSGDREHFAKEDI
                     DYIKQVLSEI"
     gene            complement(14830..16059)
                     /locus_tag="PLF90_01565"
     CDS             complement(14830..16059)
                     /locus_tag="PLF90_01565"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOK56005.1"
                     /translation="MDEIFDCIIIGGGPAGSFSTIFSSERGKKVAIIDKNAQICKKLL
                     LTGKGKCNLTNMNEINDDYLSKYRNGKFLINAFHQFSNHDLFNFFESNNLKLKIDTGK
                     RVYPESENAEDVIETIRKILDRNKVRIFLKEKVVDIKKEKNFFEIKTDKRIFRCEKVI
                     ISTGGKSFPETGSEGDGFIFASKFGHKIIKPVPALCGLEIKENFINKWQGINLKNILV
                     SAYLNDKKIGEEFGEVLFTHYGVSGPAILNISGDVSENIDKGEIKIVINFKPALNFEI
                     LDRRLQREIKEDSNKILKNLFKNFLPSGLIEEFLKNAGVDSEKKAHQITKVERIILIK
                     KLLNFELTVKRIRPFYDSMVTRGGVCTDEINPKTMESKILKGLYFAGEVIDIDGKTGG
                     YNLQACFSTGYVAGKNI"
     gene            complement(16062..16997)
                     /locus_tag="PLF90_01570"
     CDS             complement(16062..16997)
                     /locus_tag="PLF90_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK56006.1"
                     /translation="MERIVFGPVPSRRFGKSLGINNVPVPKKCSFSCVYCQIGKTEYY
                     QTERKKFYEPEEIKKQVKEVIESLKGKEIDYISFVPDGEPTLDINLGKEIEILKGFGI
                     KIAVITNSSLIWRKDVRDDLKKADCVSIKVDALSEDIWRKINRPYKSLKLNEILEGIK
                     IFSKEFKGELLTETLFVKNLNDSVEEIEKIGEFLKEVKPKIAYIGVPTRPPAEKWVEV
                     PEKEKIDFAYIVFRNKNLNVKIIEKSKDEKFGFTGKLIEDILSILSVHPMSGKEIENF
                     LRENGGEFELIEELIYKGEIEEIEYMGEKYYRRKF"
     gene            complement(17039..19972)
                     /gene="mfd"
                     /locus_tag="PLF90_01575"
     CDS             complement(17039..19972)
                     /gene="mfd"
                     /locus_tag="PLF90_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOK56007.1"
                     /translation="MNFCFLEENRKFKEILDLIKREEKLNIVGGDISFFAFLISLLFN
                     KTEKNFLVVCPSEKKSEVFYYDILNFVDKKNIKFLPSPDYNSEEPTETDFERIKTLIE
                     ISKAEEKYILISYPSAIIKKIPEFNTLKNRICEIKTGSKLRRDNFLNFLFTCGYEEKE
                     IVELPGEFARRGGIVDVFPLNSPFPLRINFSGNKIESIRRFEVSSQVSFEKIDEFQIL
                     PLNEFYAGEEKSNILEEIRNKVVFFINPDNVKFEYIEWIKRKNYPVDFDILERVKEKT
                     IFLTSRILSYAEKFVFFNVSPTNERFKIISEQVIWQQNQGEEIFLFYENKSDIERLKS
                     ILEEKKLSFDFEEVSGNLSSGFSVLDENLTFLTVNEIFSRYKARHPLLKKYKTIPLTG
                     WSEIKEGDYVVHINEGIGKFKGVERIKVNEKEEEFIVIEYEGKDRLYVPVSQVSLVHK
                     YIGSEKPKLSKLGSKTWRNLKEKVKNSIKDLAYELYKLYIERKKEKGFKFLPDDQWQK
                     EFEDSFVYEETPDQLKAIEDVKRDMMSEKIMDRIVCGDSGYGKTEIAMRGAFKCVLSG
                     KQVAVLVPTTVLALQHYLTFKDRFSKFPVIIEMLSRLVPEKKQKEIIEKLKNGQIDII
                     IGTHRLLQDDVEFKDIGLLIIDEEQRFGVLQKEKIGIKFRKIDVLTLTATPIPRTLNM
                     ALSGIKDISLIETPPEGRLSVITYVGKYNENIIREAILNEIDREGQVFYLHNYIYDIH
                     RVKEKIQKLVPSARIDIAHGRMKPEEIAEVMERFTDGKIDVLIATTIVENGLDIPNAN
                     TLIVENALKYGLADLYQLRGRVGRGKWRAYAYFLIPTDVPLNEKTLKRIKAIQEFNSP
                     GSGYKIALKDLEIRGAGNILGKQQHGFIETIGFNLYCQFWKEVMSEIKGEKVEEELRE
                     VKIKIPDDYVKSSSMRFYLYREISKIKDRKDAENLINEMIDKFGYIPEDLKNQIFSLA
                     RSDLF"
     gene            complement(20019..21932)
                     /locus_tag="PLF90_01580"
     CDS             complement(20019..21932)
                     /locus_tag="PLF90_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOK56008.1"
                     /translation="MKSEKCIVIGWDAPIVKSIKKYVEEGVMPNTKRLIDEGVWGENC
                     LVPHPTITPPNWTSIVTGSWIGTHQIVCFNLLEEGQLEKTYAAFYKEDCKAEYIWEAA
                     EKIGKKTILLNYPSTWPEAVKNGIQIGGGGLGINEYRLKGTAEWGIKCDLSADLCFTT
                     EELPQSYLIEIKDIDGWKNLPDGKRFKEAELKIEFRWSDYISKEKPWYALLIDSGKGF
                     DKVGIYRERNGEPLTILEKGKWSEKIYTEIETDKGKKRVVFKIKLLELTPDGKNVKIY
                     FTPLCSLSGFGFPSGIEKEIENLEGLPMPCAFFTSYGFGWIDIETVSELIDLQNIYLG
                     ECANYLLKNKEWDLFFMHAHCPDHFYHAFINQIDKDKKVEEAERRFYISLDRMLGRIF
                     ESIDENKTLIIITSDHGAVPTSQEYREEGFDVNKILEEKGLLVTEIDESTGRKRIVWE
                     KTKAVGKLSCYVYINLKSKYPNGIVDDSEYEKIQDEVIKALYDYTHPKTGKKPVAFAF
                     KKQDARIIGLYGERIGDIVYGIYPEVNGEHGRQITTGEYGIGSMKGLFIAKGPNVKRG
                     EIVERTIWLTDIVPTICYLLDLPVPKDCDGAVLYQIFEDPDYKRKEFDKLKKNYERMK
                     KALETEKYLTHNY"
     gene            complement(21948..23357)
                     /locus_tag="PLF90_01585"
     CDS             complement(21948..23357)
                     /locus_tag="PLF90_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOK56009.1"
                     /translation="MNLIIVISDTLRRDFLGCYGNRWISTPNIDKFAENSIVFDNAYI
                     GSFPTVPNRHEILTGKFVFTYHKWAPVPEKDITIPKILKEKKYVSMGIFDTPHPFAPG
                     MNYQRDFDGWILIRGQEHDKYITEPVDVKLPCKPEKLRNPYTTVVQYLKNVSERKYEE
                     DYFCAQTFIESTRWIERNYKHEKFFLYIDTFDPHEPWDPPKYFVDMYDPGYKGEEVIY
                     PRYGSTDVLTDDEVKHCRALYAGEVSLVDRWFGYFLRRVGDTGLLKNTCIIFTADHGF
                     YLGEHNLIGKSIIKENYHTYAPLYEEVVRIPLIIYLPGRKHKRIKSYVQPPDITKTIL
                     DIAGAEIPSDIHGKSLLPLIDGKNKNIRDFVVSAPSIIHGSSAGEKITLTDKSGWCLI
                     YESEKGEKYSIKAVDSIERETLKGSKKENELYFLPEDQYQKKNLVKENKKVFESLKEK
                     LIKFLYKVKTDEKIINMWK"
     gene            complement(23359..24114)
                     /locus_tag="PLF90_01590"
     CDS             complement(23359..24114)
                     /locus_tag="PLF90_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOK56010.1"
                     /translation="MEQNLLSKGIDVLEVLKEAEGYLSFIELQRKLKIPRTTLYRILK
                     TFISRNFIIYEDKKYKLGYGFLTFTKKILSEIPLREITTPYLKKICEKAKETVELIIP
                     DKGEILYIDKIESPQSIKLVAQIGSRYKTLHASAPGKVLLAYNNYVFEKFLKNRKLEK
                     ITEKTITNKMKLKKEIEKVRENGWAYDIGEARIDVTRISAPIFDYDGNLIGIIGIAGP
                     SYRIDKKKIDEFGNYLKKVCDEISEKLGYKKEG"
     gene            complement(24402..25556)
                     /locus_tag="PLF90_01595"
     CDS             complement(24402..25556)
                     /locus_tag="PLF90_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK56011.1"
                     /translation="MTSKERVKRLIERKDIDKIPLGFYVVDYDIIERVIGRPTYVRNK
                     VKSQIALWEGKRNEVCESYKKDTVEFFKKIDCVDILTFKEAGILPTENYNPPKIEKID
                     ENTLKDENGRIYRISESTNEISCIYNPDKNKIDIDTYTPDMFKFSESDLKEPDQSIFQ
                     APDYLIENFRDEKFIAGTSGGITAFTLIGGMETGLMLYALKPEVIKAVNERNVKIQNF
                     LDKFYIRKGQDGVLLEQDMAGTKGPFISPDIFSKVCFPFLKKRVENIKKYVPYVFLHN
                     CGNNIPLMEMFIEAGIDVYQSLQTNAGMDIEYLTKRFGDRIIFWGGISTEILISGTME
                     DVRKNIRDLIEKFKKKNLRFIIGPSHSIAYGVKYDNFMTLIEEYEKNAYL"
     gene            complement(25650..29165)
                     /locus_tag="PLF90_01600"
     CDS             complement(25650..29165)
                     /locus_tag="PLF90_01600"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOK56012.1"
                     /translation="MKRSIFLIFFFSLFFYKFVFSEEFIPQILIPYSEKAPLIDGILD
                     EKGWEKACVITGFVGATGQFGGIIVPEIGVNTFAYLMHNNDNLFVGIVYKNLERKPSK
                     NYRRRDEPVYLDNPQVEIWLTPPTEGNVKCYQMIVNGYDAVFDAEHIPSLGVERTIWN
                     GNWIVKSSYKDKKWMVEISIPFESFGIKDIKKNNVWRGMIGVAWPQCSFPYTYGWYKN
                     FQAHAQFIFSQTETCSKITDLSSLLENKLKTEVEIINGENQEENFNIHVEAGNSVVYD
                     KSFKLKAKDKINIKIDEILPEIQKNTKQIFLIKIASSGKTLFLNKWNYSLYQQERKPE
                     IKVEEEKWQMKTRVNFAPISLALYTWCDILDYPHIDKLKSVIFEVVSPLDKVIVSKEV
                     EKFEYDSAESYIWLPKDLKYGKYKVYTKFLSKEGKVLEEKIDTFEHKDLKKEFVWLGN
                     NFGKNIKVAPPFEKIKIKEKDNVILEVWGREIEFKGVFPERIKNQGKEMLAGNIGLFA
                     ELEGKLIPAIISKPIRITKKSDERVEFTGEYKIGNINFKIDGYMEFDGMIYYKMLVNV
                     PEKEKISFDRIYLSIPVNENYAKYYFSTAGGWSPSYGEIGKENKSVWKSDEIADFVPY
                     ICLTDDEIAIHWFADNDHNWILGKDFPTAEILKKDKIVEMKINFVRKKGEIKDFSADF
                     GLIASPVKPLPSSWRNACLDFRPFANSKINFFYGPGHGGCPIDPHDSEKLAKAMGVYQ
                     EGKNPDEILANLPENYIDWSNPDVLKNAEKILGKNKNGVEFIKNWDKAKKDPNPYKVK
                     RCYFFNASMYFEGYKSKGFSTFFPAEWTLIPSSWFHLTPVESYRDFFAFYMELWFRHW
                     FVEGFYFDEVYHGPDYNVFNGNGKIMEDGSIRPSIPLMHQREFMKRMRQLFIDHNREP
                     FIWVHSTNYMSPYSISPADVAMFGEDKTPIPGSDIIETIPSLLLRTLGRAQKFGFIPV
                     WMVQVGRSGLSWGFFGRQTVGWFWMHDVVPEYHTAVRGRPLIVLRQGWGIDRDDVNFI
                     PFWDNEEYVKVSDDKFIVSIWTQPKGKVLIQVMNLHKSEENKTDVKITLNTKNLNLNK
                     EFKIYDLESDEKLIEWENNLREAEKLYNQDPVKNKERIKELTKDLHKLPETIKFDISN
                     LKLISQSPEFKISVPPRDFKVYIIE"
     gene            complement(29190..>30979)
                     /locus_tag="PLF90_01605"
     CDS             complement(29190..>30979)
                     /locus_tag="PLF90_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56013.1"
                     /translation="FIFLLFFNFSCSSQNKINVSFTKEGVLNLSYKDIDFVIEGKPDL
                     KEIVFEKIGKNENGERVYNFEKFKDKPVNTEWKEKEKTLIYNYPWGSISFQYNNKGNN
                     FDVVLKISNKSERTIANFDIGILNFILPEKQEIKSPFDFWWGLGYKVVRKSGNFYELD
                     TLESPGVIEVKFGKENFTDKFLLTYASVYPPLNYGLRKNQNQWEFYIKGGILAAEPKG
                     VFIPPLGLPRIYSGKDTVFEFSIRFSDINSDTDIIVSDVYKKFRDYWQPHLEWNDRRP
                     IGMIMLASSWEGHKSATNPRGYFADKTIDVFSPEGKEKFKKIAIEHAKRSVNVLKSIN
                     AQGMIVWNIEGEENPHPITYIGDPRMLKILGPEMNEIADEYFKIFRDAGLRTGVCIRP
                     TQVYFDENDKKWKHGTGSHGPMRNPLNDDFSEIWPKDLPWWQFFPIAERLSRKIEYAK
                     KRWGCTLFYMDTNGIFAPVGENVNKFEWILLWTEVLREVRKKHPDVLIIPEFPKGSAS
                     WSQIAPYSELRGGIVSTPPIIRKLFPHSFSIINVADGPIDKRRDEIVKAVKEGDILMT
                     HGWWMPKFTEEVGKIYKEVYKEGAINPWE"
BASE COUNT        10483 a         5228 c         4057 g        11211 t
ORIGIN      
        1 agagtcacaa aattattata tacaacatga gaaaggaact gacccattca gttggataaa
       61 gataatacct tttatggatg aagaaacaca aaaaaaatat ccaattgacc atgctggaaa
      121 acaagaaaca tctctgatga tggtattcta tccttccggt gtaaaaatga aaaaatatag
      181 taaagaaaaa tggtactcaa aagaagcaaa aaaagcaaat aaagaatacg gtaaaaaggc
      241 aaaaaaagaa attataaaaa gattgaaaga ggttttaaaa taatctgtca aaatgttcag
      301 aataaatcca tgcttcatac tcaaaaggta ataataattc aggtaactta aataaacttt
      361 ttcagttttt agaagtcaaa atatatggaa tggaaaagat aaagaagtta atttttgtga
      421 tattttttca ggtaataatt ttaaatagcc agaactcttt aaatttatat gaacaggaaa
      481 actggtcaac tcttgaaagt gaaatagata gatatgtctt ttcaaaactt gaaaaattaa
      541 atattaagca tgccttccct tgttcagatg aggtctttat aagaagaatt tatcttgata
      601 caattggaat gcttcctgag gtagaagaag taattgattt tctcaatgat aaaaatcctg
      661 ataaaagaaa agtccttatt gataaattac ttgaaaaaaa agaatttgct gaatactggt
      721 ctatgaaatg gtgtgatatt ttaaggataa aggcagaatt ccctataaat ctttggccta
      781 acggagttca ggcatatcat aagtggatac tcaattcttt agttgaaaat aaaccatatg
      841 ataaatttgt tagagaactt cttacttcaa gtggaagtaa tttcagagtt cctccagtaa
      901 atttttatag agcggttggg aaaagagacc cttattcaat tgcttcctgt gtgtctctaa
      961 cttttatggg tgtaagattt gagaagttgc ccgatgatat taaaacaggg atgagtaaat
     1021 ttttctcaaa ggtctcttat aaaaaaactc ttgaatggaa agaggaaata gtttatttta
     1081 atccagagaa ttcagaaaca atagaggcaa tttttcctga tggaaataaa attaaaatcc
     1141 cacctgataa agacccgaga actgtttttg ctgattggct tataagaccc gataatccat
     1201 acttttcaaa gaacattgta aacagaatat ggtcatggat ttttggaaga gggataattc
     1261 atgaaccaga tgatatagga cctgataatc caccagcaat tcctgaactt ttaaattatc
     1321 ttgaaaacca acttattaaa tcaaattatg atttaaaaca catttataaa attattttta
     1381 attcaagagt ttaccagcaa tcatttatac cgagagataa taatcctgaa ggtgagaaat
     1441 attttgctta ttatttgcca agacgacttg aggcagaggt tttacttgat ataatatgta
     1501 aattttctgg aagtggaatt gaatatgtaa gtcaaatccc tgaaccatat actttcatac
     1561 catcatctca aagaaatatt ttacttgcgg atggtagtat aacaggccct tttcttgaat
     1621 tatttggaag gcctccaaga gatacaggac ttgaatcaga aagaaataat aaaataaatg
     1681 aggcacagat gaggtatctt ttaaattcat ctgaattaca taaaaagatt caatcaagta
     1741 attatataag acaattaatg agagaggcaa aaggagatag aaaaaaaata attgaaaata
     1801 tttatctctc tttactatca aggtatccat ctaaatctga aattgaacta cttgaaggtt
     1861 attttcaaaa gcagggtata tcttttaatc aagcagttga agatattgta tgggctttaa
     1921 ttaacagtaa ggagtttttg tacaagcatt aggaggaaaa agatgaataa aatttttaaa
     1981 atttttatgg ttattttcat tgttttcaat ttttcccaaa aatcctattc gcagtcagaa
     2041 ataagaccaa aagcagttat tcaaatctgg ctttggggag gaccgtctca tcttgatact
     2101 tttgacccaa aaccaaatgc tggatacgac tacacagggc cttttaaatc aataccaaca
     2161 aatgttcctg gaatagaaat aaatgaactt cttgtaaatc ttgctaaact tgctgataaa
     2221 tattcaatta taagaagtat gacccatgga ataaatgctc atgaaactgc tgcatattta
     2281 atgcaaacag gtcattcctc agaagaaaat cttgtttatc cctctattgg agcggttgtt
     2341 tcattattta aaggatatga ctatggatac aaaggaattc ttccacctta tattgtactt
     2401 acaacttccc agggtagatt ttcagagtgt ggatttcttg ggcctaaata taaaccattt
     2461 gtaacaggtg gagacccaaa cagaacccca tttgcagttg aaggtattgt acttgaagga
     2521 atatcagaag aaagaaaaaa tgcaaggaaa gaacttttat attcaataga tatacttggt
     2581 aaattaatcc cttggagtaa agaatttcaa tggtttgatt tatgtagaga aaaagcatac
     2641 gaaataatat caggagaaat tgcaaaggtt tttgaccttt caaccgaacc agaagaggta
     2701 agaaatatgt atggcagaaa tacttttggg caatcgtgtc ttgtggcaag aagattgatt
     2761 gaaaaaggag ttgtctatgt aacaataaat tacaggggat gggatacaca taacaaccac
     2821 tttcaacaga tgagacagaa acttcctgaa cttgatatgg gactcgcttc tcttattaaa
     2881 gacctttcag atagaggact tcttgattca acaattatat gggtatcagg agaatttggc
     2941 cgtactccaa aagttcagtg ggaaccgcca tggaatggag gaagaggcca ttacggtaaa
     3001 tgtttttcag ttcttgttgc cggaggtgga tttaaaggag gatgtgttgt tggaaaaagt
     3061 gatgaaaaag gggaagaagt gattgacaga cctgtatatc caaaagattt actcggaagt
     3121 atttattatc ttttaggcat tgaccctgag ggaaaattcc ctgaaaaatt tggaaaagat
     3181 gcgagaattt tacctccaat tgaaaaggga ccaaaaaaag gacttctgaa ggagataatg
     3241 tatgaataaa agaagtattt ttttaatttt tctaatttca tgcaatttta tttttgctca
     3301 aattatgaca aatcaaccat ctattggata cgtttatcaa gcaggtggac agagaggaac
     3361 tactcttgaa ataattgttg gagggcagaa cataagagga gcaagaaatg cttatttttc
     3421 aacagataaa attaaagttc ttcaggtcaa tcctttgcct tcattaaatc cccagcaaaa
     3481 acgacttctg gcaaagaatt taagagaaat tataacaaac atgtatagaa aagaatacag
     3541 aaaaccagaa atagtttatg aaatggatgg agtgaaatta ccaaaacacc ctttacttga
     3601 aaaccttgaa aataaatcat ttgaggagtt aagatttatt gctgaaactt ttttaggtcc
     3661 ttttagaact gaacaaataa aaaggtcaat tcaggaaaaa gtaatgataa aagttgaaat
     3721 ttctcctgat acagaacctg gaatttatga attgagagtt caatcacctt taggccttac
     3781 caactttcta aatttctatg taagtgaata taaagaagta aaagaaaaag agaaaatccc
     3841 atactataca atttttgacc cacttaaaga agtttttgat attcccttaa ttataaatgg
     3901 tcagattact cctggagatg ttgacagatt ttattttaat gcaaaaaaag gacaaaaact
     3961 tgttattgaa cttaaaggac gagaaattat tccttttatg gcagatgctg ttcctggatg
     4021 gtttcagggg gttttaactc tttatgattc ttccggtaga gaaattgcat atgcagatga
     4081 ttattatttc aatccagacc ctgtaatttt ttatgaagta aaaaaagatg gtgaatatat
     4141 gatagaagtc agagatgccc tatatcgcgg aagggaagat tttgtttaca gattatttat
     4201 tggagaaaaa ccattcataa cacatatttt cccaccaggt ggagaaagat atgaaaaaac
     4261 aattgtttca atatatggat ggaatttacc ccaaaaaaca gttgaacttg atacagaaga
     4321 tatagggatt catagaaaaa gttttgactt taacggtata tattcaaatc aaatatatta
     4381 tatggtggac gaaatacctg aaataaatga aagagaacca aatgatagtt tgaaaaatgc
     4441 aatgcaaatt ataatgcctc aaatagtaaa tggaacaatt tcaaaaagtg gagatattga
     4501 tatatataaa tttaaatgtt caaaaggtga taaaattgtt gcagaagttt atagccgtcg
     4561 tttaaatttt ccacttgatt caattattac acttatggat tcctctggaa aagttctaat
     4621 ttcaaatgat gactattttg acaaaagttt tgatacaatt acacatcatg ccgactctta
     4681 tatttattat gaaatcccgg atgatggaat atattacttt aaaattacag atgtaaaagg
     4741 tcatggtggc gatgaatata tctatcgttt aagaattgag aaaagcaaac cagattttaa
     4801 aatatttata gttccttcag ccataaatat tacatcaaac aatacagttc ctttttatat
     4861 ttatgctgtt aggaaagatg gatttgatgg agagatagaa gtttcgttaa aggattgtcc
     4921 tgaaggactc attttaagtg gaaataaaat tccaaaaggt aaggataaaa tatgtatgac
     4981 aattactgct gataccaatt tcaacttcct taataaacct gttaggataa aaattgaagg
     5041 aaccgtaaat ataaatggaa aacttataaa gagagaagca attccagcag atgaaatgat
     5101 gcaggcattt gcatattatc atcttcttcc ttcttccgaa ttactctttt atcctggtag
     5161 aagcagattt tttaagagtc aacctatatc tttgaatgaa aaatttataa aaattccttc
     5221 cggtggttca gttaaaattg agggtaaaat ttcaggatat tctgaaaatg aaaaaattac
     5281 acttgaattg aaagacccac ccgatggaat aacaatagaa gatttaaaaa ttgaaaatga
     5341 tactgtaagt ttcagaataa aggcagaacc taaaataaaa aaaggatttt cagacaattt
     5401 gataattgag gttttcagtg aaccaaagga agaaacatct caaaaaaaac aaagaagttc
     5461 aaaaggattt ctgcctgcat tgatgtttga aattatttga aaggagaaaa atggaaaaaa
     5521 ataaaattga aattaaagga ataccagttg agataaaata ttcttctttt aaaggaaaat
     5581 caggagttga agaatttcat ttcagtttta caccaaaaga atttggagat ttcagaacac
     5641 aacttaaatg gatatacaat gcttatactg aaacacttaa agaaaaagga ataagtgagg
     5701 agacatctgt ttttagaaga tttttctgca gtgatttata caatcaggta gaatatttaa
     5761 gagaaatgga cttttcaaat ccagaaaata tttcaaatcc aacttgcatt tcatggatat
     5821 gtcagccacc tcttccctat tcaaaaataa gtttgtgggc ctatcatata aaagaccctg
     5881 aaaacaaact gaaaaaagaa ttaaaaggaa aaaatacctg ctgtattata agagaagata
     5941 ttattcatta ctggacaggt ggtattttct caatagaaaa agattctata tacgaacaga
     6001 caaaaaatat aattgaaaaa tatgtggaat tcttgaatga aaataatatg aaactaagtg
     6061 ataatgtgat aagaacatgg tttttcttgc agaatattga tacggattac agggactttg
     6121 tagttgcaag aagagaaatt tatgaaaaaa acggtttaac aaaaaataca cattatattg
     6181 taagtacagg agttggtggt tcttattatg acttaaaagc aaaagtaatt ttagatgcgt
     6241 attcaattta cggaataaaa acacagcaga taaaattttt atcagcacct gattttttaa
     6301 gtcccactta tgtttatgga gtcacatttg aaaggggtgt ttcaataagt tattcagaca
     6361 gaaaacacat aataatttca ggaacagcaa gtataaacaa taaaggtgaa attatgtacc
     6421 caaaagatat aaatttacag acagaaagaa cccttttaaa tatagaagca ctcttgaaaa
     6481 acgaaaatgc aactctaaat gatatgatga tttttattgt atatgttaga gaccctgctg
     6541 attattattt tgttgaaaag aagataaatg aaagatttaa aaacacacct accattttta
     6601 caactgcatc tgtatgtcgc cctggctggc ttgttgaaat agaaggaatt gcagttatcc
     6661 cctccccgcc actgcccacc tttaaggtgg acagataaaa tgattggaag gcaagggttt
     6721 tgaagtacgt ttttttgaaa tttgacaaat ttgaaaaaag tgataaattt aaaaacttaa
     6781 aacggaggaa aaaatggaat ttaaaataaa gaataatcag aaaattttat ttattggaga
     6841 cagtataact gattgtggaa gaagagaaca attcagaccg cttggaaatg gatatgtaaa
     6901 acttttttct gaccttgtta ttgcgaattt tcctgaaaga aaaatagaaa tagttaataa
     6961 gggtataagt ggaaatacaa ttcttgacct tgcaaataga tgggaagatg atgttattta
     7021 tcagaaatgt gactggttga gtattttaat cggaataaat gatatacaca gagtccttag
     7081 aaaagatgaa atgtggactg aatttacacc tgaaaactat agaaaaagat atgattatat
     7141 tctcaaaaga actcttgaaa aacttgactg taaaataatt ctgattgaac cgttttatat
     7201 ttcaacagat acaactgata gtttcagggg aaaagtttta aaggatttga tagaatatat
     7261 tgaaatagtt gaagaaatga gtaaaaaata cgaaacatat catttaaaat tacacagaat
     7321 ttttcaggaa tatcttaaat tttttgaacc tgagacattc gctccagaac cagttcatcc
     7381 aaattcaaca ggtcattttg taattgcaaa tgaacttatc aaattactct cagaataaac
     7441 tgctaatttt ttcctgaagt tttattattt cttcaagttt atcttcagga acatatttca
     7501 ttctggatat ttcctctctg actggatttt taagtatttc ttcaagtgtt cttccttttt
     7561 ctattgcttt tagtgatacc cgataaaaca gaataattgt tttcagcata agatactgtt
     7621 tttttaaagt tgtataagca tccatttcgt caaaagcact ctgatgtaaa aaatcctctc
     7681 ttatagaacg agcaatttca agtattaatc tatctgtaag agaaagtgtt tccataccaa
     7741 caagtcgtac aatttcaagt aattctgctt ctttctcaag taaggatatc gcctctgctc
     7801 ttatttccat aaaatcagaa gcaatttttt ctttaacaaa tttttcaata ttcctttcat
     7861 aaagagaata acttgtaagc cagtttatcg caggaaaatg tctctgatat gcaagacggt
     7921 catcaagtcc ccagaaaacc tttaccaccc ttaaagtcat ctgtacaaca gggtctgtta
     7981 aatcaccacc aggagggctt actgctccaa gtaatgaaag agaaccattt ttattatccc
     8041 gtgccaaaca tataacattt ccggctcttt cataaaaaga ggcaaccctt gttccaagat
     8101 atgcaggata accttcttct cccggcattt cttccattct tcctgatatt tctctcaatg
     8161 cttcagccca tctacttgtt gaatcagcca ttaatgcaac tgaataaccc atatccctga
     8221 aatattctcc aatagtaaca cctgtataaa tacttgcctc tcttgcagca acaggcatat
     8281 ttgaagtatt tgctataaga catgttcttt ccattaaagg tctgcctgat ttagggtctt
     8341 ttaaatgtgg aaattcaatt aaaacatctg ccatttcatt tcctctttca ccgcaaccta
     8401 tatatacaac aatttcacta tcagcccatt ttgcaatttg atgaagaaca acagtttttc
     8461 cactaccaaa aggtccagga atacaggcag ttccaccttt taaaatagga aataaagtat
     8521 caataaccct ctgccctgtt acaagtggag tatcaggatt taatcttttt ttgaaaggtc
     8581 ttggttttct tacaggccat ttctgcatta agtataaagg aatctctcct ttatcagttt
     8641 ttactttccc tatttcttcc tcaactgtaa aacttccttc ctttatttca ataatctcac
     8701 cattaactcc gtaaggtacc ataattttat gctttataag aggtgtttca tcaacaattc
     8761 caataacatc cccactttca acataatcgc cttttttaac aaccggttta aaatcccatt
     8821 taatacttct atcaagaggg ggtatatcaa ctcctcttat tataaaatcg cctgtttttt
     8881 ctatctggga taggggcctc tgtataccat caaaaatatt ttgaagaagt cccggtccaa
     8941 gttcaactct caatggttct cctgtaccga aaacagggtc tccaactttt aaaccctctg
     9001 tttcctcata tacctgtatt gaagcaatat ctccttcaag tcgtataatc tcaccaataa
     9061 gtttttcctc cccaactctc acaacatcaa acatcttact tgttttcatt ccttctgcct
     9121 gaacaagagg tcctgaaact tttataattt ttcctatatc cattttaatc tcccttcata
     9181 cctgttgcct ttttaatcaa ttcatcaaca atttttactc cctttccttc cctactcttt
     9241 aaccctggaa taactaccat ttttttcata ttcaaaaatt tcataaactt atcttcatca
     9301 aaaacttcct ctgtaataaa aatacatatt acatccttca tatcaatttt ttcaagtatt
     9361 tcttctgctt ctttaactga atttgcaaaa aatgtttcta tattaaaagc agaaaaaaat
     9421 tcaatactca atttatctcc aataaaaatt attctgtttt ccatatagat taaaaattta
     9481 ccctaaattt cattttattt caatttttta tgaaattaaa aattttgttt taaaaactgg
     9541 tctttaaatt ccttgtcatt cctactttcc ccaagcccac ctttaaggtg ggtaaaggtt
     9601 attgtaaagt ttttatgtaa aatgtaaaat aaaacttaat atgagaaata aaattttttt
     9661 tgtttttttg ttattggtca gttttttata ttgtgaaaaa aatttagtta ttatttctcc
     9721 gcactgggaa ggagttaaga ttgaatttac gagggctttt tgtgaatggt atgagaaaaa
     9781 ttataaggaa aaggttaaaa ttgaatggat tgaccagggc ggaacaactg atgatttgaa
     9841 atatgtagaa tcacttttta aaaaaaatcc agatggaatt ggaattgatt tgttttttgg
     9901 agggggatta agtccttact tgacattaaa aaaagaagga ctgcttgaaa aatataaact
     9961 tccgcagaat gttctaaaaa aaataccgaa atactgtgct ggaattccca attatgatga
    10021 taatttctac tggtatggag ttgtactttc aggttttgga attctttata ataagaaagc
    10081 actgaaattt ttaaatttac ctattgcaaa tgcatggaag gatttgggag atagtcgtta
    10141 tttttcttta attggagcag cagaccccag acatagtgga agtatgcata tgatgtatga
    10201 gataattttg cagacatatg gatgggaaga aggctggaaa actattcttt caataggtgg
    10261 aaatacaaaa aatttttcaa caagtgcctc cgaagtagca agagagacat caattggaga
    10321 agcagttttt tctttatgta ttgattctta tgcacttgct caaatagaag taaatggaaa
    10381 agaaaatatg ggatttattt tcccagaaag tcaaactgtt ataaatcctg attgtatagg
    10441 aattcttaaa ggagcaccaa atattgaaat agcaaaaaaa tttattgatt ttttattcac
    10501 ttatgaaggg cagttattat ggattcagaa aaaggggaaa aaaggtggac caaaagaata
    10561 ttcacttaac aggtttcctg ttatgcctga agtttataat gaaccaggtc tggaaataga
    10621 tataaaccct tacagaattc agaatacaat aaaatatgat ttccaacttg ctgcaaatag
    10681 atgggcactc atagatgata tgattgggtg ttttgttatt gactgtcata gtgagttaaa
    10741 aaaagcatgg catataattt caaaaacaaa taataaaaat ttaaaagaaa gattttatga
    10801 aatccctgtt gataaaaaaa ttcaaaggtt tttatgggaa aactggaagg acccgatttt
    10861 cagaaataaa tatataaatg aatggctaaa tttttctctt aaaaaattca gaaatattat
    10921 tgtggaaagt aaatttttga tgtaaattaa aaaatatgga taaagaaatt ttaactgttg
    10981 aaaatgttga taaaagttat aatggtaagg aagttttaaa aaatatcaat ttttctgttt
    11041 atgagaatga attttttttc atactcggac cttctggttg cgggaaaaca accctcttaa
    11101 gaattattgc tggatttgtg gaacctgata gaggaagaat tattctgaat aaaaaagatg
    11161 tgacaaaaac ccctccaaat aaaagaaata ttggatttgt cttccagaat tacgccttat
    11221 ggcctcatat gaaagtaata gacaacattg catatggact cagaataaaa aaatattcag
    11281 aagagtttat aaaaagaaga gtgaaagaaa taattgaaat aacaaaattg aaaaatgttg
    11341 aaaatcttta tccttctcag atatcaggtg ggcaacaaca gagagttgca cttgcaaggg
    11401 ctcttgtagt caatcctcag attttacttc ttgatgaacc gctcagtaat cttgatgcaa
    11461 aattgagaga tgaattgaga agggaaataa aaagaataca caaagaaact ggaataacaa
    11521 tggtatatgt aactcatgac caaaaagaag caatgactct cggtacaaca attgcagtta
    11581 tgaaagaaaa agaaatagtt cagattggtt ctccatatga aatttactca aatcctgaaa
    11641 atctttttgt tgcatctttt atcggtgata ttaactcttt aaaagggaaa atagttgaaa
    11701 agaaaggaaa tattttaaaa attgaaacag atgaaggttt tttctttgct aaaattaaaa
    11761 aaacaataaa ttctgaaaat gtttatttat gtttcaggcc ggaaagtata actgttgagg
    11821 aaaaaataaa taccataaca ggaagattgc atgattatga attttatgga gatattgtga
    11881 aaataaatat aattactcca aaaggaaata cttttaaaat tggaatttat gcagatgatt
    11941 ttaataaata taaaaaagac gaaaatattg ttttttcagt taaagaagat aaaataatgg
    12001 tttttgaaga atgaaaagga aaaaaatttt attaacattg atttttttgt tttttttact
    12061 ttttcttgtt tatcctgtat cctttattct catccagtgc ttttataata atggaaattt
    12121 cacacttgaa atacttaaaa tcgcaattac aaattatgtt ataagaaaat caattatcaa
    12181 cagtttcatc cttggaataa ttgtcgttct tacaacaagt ataattggaa ttccacttgc
    12241 ttttatattt gatagattaa attttaaggg aaagtcaatt ttaagaattt tatttttact
    12301 tccaatgatt gcaagcccat ttgtaggaag catagggata aaacagtttt tatcaagatt
    12361 tggaagtttc aatattctac taattaaact tggaatattg aaaaatccaa tatcctggat
    12421 tggctctggc ttccttggaa ttgtaattct tcagacactt catttatatc caattatgtt
    12481 tctgaatatt accgcctctc tgaataacta tgatatatca gtagaagaag catcttataa
    12541 tcttggagca actggattac agacattttt caaagtaata tttccattga ttttaccagg
    12601 atattttgca gcaagttcaa taatattcat ctggtcaata actgacctcg gtactcctct
    12661 tgtatttgaa ttcagcaatc ttgtttctgt aaaaattttt tatacactta aagatataaa
    12721 tacaaatcct gttggttatg cccttgtttt tataattctt ttgctgaccc ttcttctttt
    12781 tatttttaca aaaagatatc ttgaaaaaac accatacaca acaggaaaaa ctgcaattta
    12841 cgtaaaagaa aaaaatattg tgggtagaaa actctttttt ctttaccttt tcctcttttt
    12901 catactcttt ttaagtttaa taccccactt cactttaata ttttattctt tttcaaaaaa
    12961 gtggtttttt tctatcctgc ctgctgaata tacaggtgaa tattataaag ttgttttcgc
    13021 tcacagatta acaaaaacag gaatttttaa cagtttaatt tacagttcaa taagtacgac
    13081 catagatatt tttcttggat tttttattgg atatatactt gcaagatgga aaataaaagg
    13141 gaaggaattg cttgacatat tatcaatggt tcctcttgtt attcctggta ttgtaatggc
    13201 atttggatat gttgctactt tttcaaatac aattttagac cctaaaaata atcctgtatt
    13261 tcttatagtt ttaagctaca gtttaagaag attaccctat cttgtaagaa ccacttattc
    13321 ttcattttta caacttgata gaggacttga agaagcatcc tattcacttg gaggagataa
    13381 tatcataaca ttcaaaaaaa ttatatttcc tcttgtaagt cctggacttt ttgcaggtgg
    13441 aattcttgct tttgctttct ctctgatgga agtaagttgt agtttaatac ttgcaataag
    13501 agaaaaattt tatccaattt caaaagttat atatacactt gctggaagag taacagatgg
    13561 tccaaataca gcaacctctc ttggagtcct tggaatgtta ataacaggaa tatgtctttt
    13621 catttcaaca aaaatatttt caaaaaaaat tggagaattt ttccgcattg gttaaattta
    13681 tctacttttt cctttgaaaa attgaaataa tagaaatatt taaggtataa ttaaaatgat
    13741 gattgatttt aacagtcctg aatggaagga aagattaaaa gggaaaacac cggaagaaat
    13801 tgctaaaatt gttgaagagt attatgaaag aaagtacaaa aaagaatatc ctgtttttcc
    13861 gtggaaaatt ctacttgcta taataattct tttcattctt ttgattataa ttctctgggg
    13921 agcaatttat taccttaaac gagtatcatt ttaaatttcg gataaaactt gtttaatata
    13981 atcaatatct tcttttgcaa aatgttctct atctccagac ctcggtgctt caagacagag
    14041 aaaacctgta taatttttaa ttttaagtat tttaagaaat tctctgttat taataattcc
    14101 atctgcaagt gaacaattta tataattttt gtattcaata ccttctattt tgaaaagatt
    14161 ttttaagtgg atatagtata acttttctcc acacatttct attgcatcca ttagtgaaac
    14221 aggattttta aaacatacaa tattagcata atctagattt attcctacac ttttcttatc
    14281 tattaaatca acaagttttt ttgtactttc cacagtatca tgaagataac acatatgtgt
    14341 ttcaaaggcg aatttaaaat ttaattcttc agcaacatcg ccaagtatct taaatccact
    14401 taccgcccat tcccagtgtt cttctttagc aatatttgaa ccatgcttat caaattcaaa
    14461 atatgagaaa gaaggattaa caagaactcc tgtcattgta ttacagatag tcaaattaaa
    14521 atatctggat gcatttctgt aaaaattgac tgcctcttct atttctcttt ctcttttatc
    14581 tttttcaggt atcattaaat ttggtcccgg agccccaaat aaaacatatt caagttttga
    14641 tttttccact gcttttgcaa gtttttcaag atactcttct tcatccatat ttttcatttt
    14701 tcttctgaat tcaactccat catatccaag ttttactgcc ttttcacaga tttcttctat
    14761 ggactgaccc tgttccatat aattcacatg cataataatt ttattttcca tattttctcc
    14821 tcctttttat taaatatttt tacccgcaac atatccagtt gaaaaacatg cttgtaaatt
    14881 atatccacct gtctttccat ctatatcaat aacttcccct gcaaaataaa gtccttttaa
    14941 aatttttgat tccattgtct ttggatttat ctcatcggta caaactcctc cccttgtaac
    15001 catactgtca taaaaaggtc ttatcctttt tactgtcaat tcaaagttta aaagtttttt
    15061 tataagtata atcctttcta cctttgttat ctgatgtgcc tttttttcac tgtcaacacc
    15121 tgcatttttc aaaaattcct caattaaacc agaaggaaga aaatttttaa aaagattttt
    15181 aagtatttta tttgaatctt ccttaatttc tctctgtaat cttctgtcaa gtatctcaaa
    15241 atttaaggca ggtttaaaat ttattacaat ttttatctct cctttatcaa tattttcaga
    15301 aacatctcca cttatattca aaattgcagg accactaact ccataatgag tgaataaaac
    15361 ctctccaaat tcttcaccaa tctttttatc attcaaatat gcacttacaa gaatattttt
    15421 cagatttatc ccctgccatt tatttataaa attttccttt atttcaagac cgcataaagc
    15481 aggaacaggt tttattatct tatgtccaaa ttttgaagcg aaaataaacc catctccttc
    15541 tgaacctgtt tctggaaaac ttttaccacc agtggatata ataacttttt cacatctgaa
    15601 aattcttttg tctgttttaa tttcaaaaaa atttttctct ttttttatat caacaacttt
    15661 ttcctttaaa aaaattctta ccttatttct atcaaggatt tttcttattg tttctatcac
    15721 atcttctgca ttttcacttt ctggatatac tctttttcct gtatcaattt taagttttaa
    15781 gttattactt tcaaaaaaat taaaaaggtc atgatttgaa aactgatgaa aagcatttat
    15841 aagaaattta ccatttctgt attttgaaag gtaatcatca ttaatttcat tcatatttgt
    15901 gagattacat ttccctttcc ctgtaagtaa aagttttttg catatctgag cattcttatc
    15961 tattattgct actttctttc ctctttcaga agaaaaaata gttgaaaaac ttccagcagg
    16021 tcctcctcca ataattatac aatcaaatat ttcatccata tttaaaactt tctcctgtag
    16081 tatttttctc ccatatactc aatttcttca atctctcctt tataaatcaa ctcttcaatt
    16141 aattcaaact ctccaccgtt ttctctcaaa aaattctcaa tctctttccc gctcattgga
    16201 tgaactgata aaatacttaa aatatcctca attaattttc ctgtaaatcc aaatttttca
    16261 tcctttgatt tttcaataat ttttacattt aaatttttat ttctaaaaac aatatatgca
    16321 aaatcaattt tttctttttc tggaacttca acccattttt cagcaggtgg tcttgttgga
    16381 acaccaatat atgcaatttt tggttttact tcttttaaaa attcacctat tttttctatc
    16441 tcttcaacac tatcatttaa attttttaca aaaagcgttt ctgttaaaag ttctccttta
    16501 aattcctttg aaaatatttt tatcccttca agtatctcat ttaattttaa acttttatag
    16561 ggtctattta tttttctcca tatatcttca cttaaagcat ccacttttat agaaacacaa
    16621 tctgcttttt ttaaatcatc ccttacatcc tttctccata tcaaagaaga atttgtaatg
    16681 actgctattt ttattccaaa tccctttaaa atttcaattt ctttcccaag atttatgtca
    16741 agagttggtt ctccatcagg aacaaaactt atataatcaa tttctttccc tttcaaactt
    16801 tcaattactt cttttacctg cttttttatt tcttctggtt cataaaattt cttcctttct
    16861 gtctgataat attctgtttt ccctatctga cagtagacac aggagaaact acatttttta
    16921 ggaaccggta cattattaat tcctaaactc ttcccaaatc tccttgaagg cacaggccca
    16981 aaaactatcc tttccataga aaagaattat aatacatttt taacctgaat accatttttc
    17041 aaaagaggtc ggaccttgca agagaaaaaa tctggttttt taaatcttcc ggaatatagc
    17101 caaatttatc aatcatttca tttattaagt tttctgcgtc ctttctatcc tttatttttg
    17161 aaatttccct gtataagtaa aatctcatag aagaagattt aacataatca tcgggtattt
    17221 taatctttac ttctctcaat tcctcttcaa ctttttcacc ttttatttca ctcataacct
    17281 ctttccagaa ctgacagtat aaattaaatc caattgtttc aataaatcca tgctgctgtt
    17341 taccaagtat attccctgct cctcttattt caaggtcttt caaggcaatt ttgtatccac
    17401 ttccaggact gttaaattcc tgtattgctt ttattctttt caatgttttt tcattcagag
    17461 gtacatctgt aggtatcaaa aaatatgcat atgcacgcca ttttcctctg ccaactcttc
    17521 ctcttaattg ataaaggtca gccagtccat attttaaagc attttcaact attaaggtat
    17581 ttgcattagg tatatcaagt ccattttcta caattgttgt tgcaattaaa acatctattt
    17641 tcccatcagt aaatctctcc ataacttctg caatttcttc gggtttcatc cttccgtgag
    17701 caatatctat tctggctgac ggaacaagtt tctgaatttt ttctttaact ctgtgtatat
    17761 cataaatata attgtgaaga taaaagacct gtccttctct atcaatttca tttaaaattg
    17821 cctctctgat aatattttca ttatactttc ccacatatgt aataacagat aatcttcctt
    17881 caggcggtgt ctctattaaa gaaatgtctt taattccact taaagccata ttaagtgtcc
    17941 ttggaattgg tgttgctgtt aatgttaaaa catctatttt tctgaattta atccctattt
    18001 tctccttctg aagtactcca aatctctgtt cctcatcaat aattaaaagc ccaatatcct
    18061 taaattcaac atcatcctga agcaatctgt gagttcctat aattatgtca atctgaccat
    18121 ttttaagttt ttcaattatt tctttctgtt ttttttctgg aacaagacgg gaaagcatct
    18181 caataataac aggaaatttt gaaaatctat ctttaaatgt aagataatgc tgtaatgcaa
    18241 gaaccgttgt tggaacaaga actgctacct gttttccact caatacacat ttaaatgcac
    18301 ctctcatggc aatttctgtt tttccatatc ctgagtcacc acatacaatc ctatccataa
    18361 ttttttcact catcatatcc cttttaacat cttctattgc ctttaactgg tccggtgttt
    18421 cctcataaac aaatgaatcc tcaaattctt tctgccactg gtcatctgga agaaatttaa
    18481 aacctttttc cttttttctt tctatataaa gtttataaag ttcataagca aggtctttaa
    18541 ttgaattttt taccttttct ttcagatttc tccatgtttt actgccaagt ttggacaatt
    18601 ttggtttttc acttcctata tatttgtgaa caagagaaac ctgagaaaca ggaacataca
    18661 atctatcctt tccttcatat tcaataacta taaactcttc ttccttttca ttaactttta
    18721 ttctttcaac tcctttaaat tttccaatac cttcatttat atgcacaaca taatcacctt
    18781 cttttatttc agaccaacct gtaagaggaa ttgttttata tttctttaaa agtggatgtc
    18841 ttgctttgta tcttgaaaaa atctcattaa ctgttaaaaa ggttaaattt tcatccagaa
    18901 ctgaaaatcc acttgataaa tttccactga cttcttcaaa atcaaatgat aattttttct
    18961 cctcaagaat acttttcaat ctttcaatat cagatttatt ttcataaaaa agaaaaattt
    19021 cttcaccctg attctgttgc catataacct gttccgatat aattttaaat ctttcatttg
    19081 ttggagaaac attgaaaaaa acaaactttt ctgcataact taaaattcta ctggttaaaa
    19141 aaattgtttt ttcttttact ctttcaagta tatcaaaatc aacaggatag ttttttcttt
    19201 taatccactc aatatattca aattttacat tgtcaggatt tataaaaaaa acaactttat
    19261 ttctgatttc ttccagtata tttgattttt cttctcctgc ataaaattcg tttaatggta
    19321 aaatctgaaa ttcatcaatt ttctcaaagg atacctggga agaaacctca aaccttctaa
    19381 ttgattcaat tttattacca gaaaaattta ttctcaaagg aaatggagag tttaaaggaa
    19441 aaacatcaac aatgcctcct cttcttgcaa attctccagg tagttcaact atttcctttt
    19501 cctcatatcc acaggtaaaa agaaaattta aaaaattatc ccttcttaac ttgcttccag
    19561 tttttatttc acaaattcta ttttttaaag tattaaattc aggtattttt tttatgattg
    19621 cagagggata agaaataaga atatattttt cttctgcttt tgaaatttct attaatgttt
    19681 ttattctttc aaaatctgtt tctgtcggtt cttctgaatt ataatcaggg gaaggtaaaa
    19741 atttaatatt ttttttatca acgaaattca aaatatcata ataaaatacc tcactctttt
    19801 tttcagaagg acaaacaaca agaaaatttt tttcagtttt attgaaaagt aaggaaatca
    19861 aaaaggcaaa aaaagaaata tccccaccaa caatatttaa tttctcttcc ctttttatta
    19921 aatccagaat ttctttaaat tttctatttt cttctaaaaa acaaaaattc atataaaaat
    19981 aatacccttt atcactcaaa atttaaacag gggaaatttc aataattatg ggtcagatat
    20041 ttttctgttt caagtgcttt tttcattctt tcataatttt ttttcagttt atcaaattct
    20101 tttcttttat aatcagggtc ttcaaaaatt tggtataata cagcaccatc acaatccttt
    20161 ggaacgggta aatcaagcaa ataacatatt gtaggaacta tatcagtaag ccaaattgtt
    20221 ctctcaacta tttctcctct ttttacattt ggaccttttg ctataaagag acctttcata
    20281 gagccaattc cgtattctcc tgttgttatc tgtcttccat gttctccatt aacttcagga
    20341 taaataccat aaacaatatc gcctattctc tcaccatata aacctataat tcttgcatcc
    20401 tgttttttaa atgcaaaggc aactggcttt ttccctgttt ttggatgagt ataatcatat
    20461 aatgctttta taacttcatc ctgtattttt tcatattcac tatcatcaac tattccattg
    20521 ggatatttac tttttaagtt tatgtataca taacaggata atttaccaac tgcttttgtt
    20581 ttttcccaaa caattctttt tctacctgtt gattcatcta tttcagtaac aagtaatcct
    20641 ttctcttcaa gaattttatt cacatcaaaa ccttcttccc tgtattcctg agatgttgga
    20701 actgctccat ggtcagatgt aattattata agtgttttat tttcatctat gctttcaaaa
    20761 attcttccga gcattctatc aagagaaata taaaaccttc tttctgcttc ttcaactttt
    20821 ttatctttat ctatctggtt gataaaagca tgataaaaat ggtcaggaca gtgagcatgc
    20881 atgaaaaata aatcccattc tttgttttta agtaagtaat ttgcacattc tccaagataa
    20941 atattctgta aatcaatcaa ttctgaaact gtttcaatat caatccagcc aaatccatag
    21001 gaagtaaaaa aagcacacgg cattggaaga ccttcaagat tctcaatttc cttttcaata
    21061 cctgaaggaa atccaaaacc tgaaagagaa cataaaggag taaaataaat ttttacattt
    21121 ttgccatcag gagttaattc aagaagttta attttaaaaa caaccctctt ttttccttta
    21181 tcagtttcta tttcagtata aattttttca ctccattttc ctttttcaag aatagtaagt
    21241 ggctctccat ttctttctct gtaaatccca actttatcaa aaccttttcc tgaatcaatt
    21301 aaaagagcat accatggttt ttcttttgat atataatcac tccatctgaa ttcaatcttc
    21361 aattccgcct ctttaaacct cttcccatct ggcaaatttt tccatccatc aatatctttt
    21421 atttcaatca aataactctg tggtaattct tcggtcgtaa aacataagtc agcacttaaa
    21481 tcacatttta ttccccattc agcagttccc tttaatctgt attcattaat tccaagtcca
    21541 cctcctccta tctgaatccc atttttaact gcttctggcc aggtacttgg ataattcaga
    21601 agaattgttt ttttacctat tttttctgct gcttcccaga tatattctgc tttacaatct
    21661 tccttataaa atgctgcata ggttttttca agttgtcctt cttcaagtaa attaaaacaa
    21721 acaatttgat gggttcctat ccaactgcct gtaacaattg atgtccagtt tggtggtgtt
    21781 attgttggat gaggaacaag gcagttttct ccccagacac cttcatcaat caatcttttt
    21841 gtatttggca taacaccttc ttctacatat ttctttatac ttttaacaat aggtgcatcc
    21901 catccaatta ctatacattt ttcacttttc attctttccc tcctttttta tttccacata
    21961 tttattattt tttcatctgt tttgacctta taaagaaatt ttattaactt ttcttttaaa
    22021 ctctcaaaaa cttttttgtt ttcctttacc aaattcttct tctgatactg gtcttctggc
    22081 aggaaataaa gttcattttc ttttttgctt cctttaagtg tctctctttc aattgaatca
    22141 actgccttta tactgtattt ttcgcccttt tcactctcat aaatcaaaca ccaaccactt
    22201 ttatcagtta atgttatttt ttcaccggca gaagaaccat gtattataga aggtgctgaa
    22261 acaacaaaat cccttatatt tttatttttt ccatcaatta aaggtaaaag tgatttccca
    22321 tgaatatcag aaggaatttc tgcccctgca atatcaagaa tggtttttgt tatatcaggt
    22381 ggttgaacat atgattttat tcttttatgt tttcttccag gtaaataaat tataagagga
    22441 attcttacga cttcctcata taaaggagca taagtatgat aattttcttt tatgatagat
    22501 tttcctatca gattgtgttc tccaagataa aatccatggt cagcagtaaa tataatacag
    22561 gtatttttta aaagacctgt atcccctacc cttcttaaaa aatatccaaa ccatctatca
    22621 acaagagaaa cctcaccagc atataaagcc ctgcaatgtt ttacttcatc atctgttaaa
    22681 acatcagtac ttccatatct tggataaatc acctcttcgc ctttatatcc cgggtcatac
    22741 atatcaacaa aatattttgg tgggtcccat ggctcatgtg ggtcaaatgt atctatataa
    22801 agaaaaaact tttcatgctt gtaattcctt tctatccatc ttgtactttc aataaatgtc
    22861 tgggcacaga aataatcttc ttcatacttt ctttcagata catttttaag atactgaaca
    22921 actgtagtat atggattcct taacttttct ggtttacatg gtaatttaac atcaactggc
    22981 tcggttatgt atttatcatg ctcctgtccc cttattaaaa tccaaccatc aaaatctctc
    23041 tgataattca ttccaggtgc aaatggatgt ggagtatcaa atatacccat acttacatat
    23101 tttttctctt ttagaatttt agggattgtt atatcttttt caggaactgg agcccactta
    23161 tgatatgtaa aaacaaattt acctgttaaa atttcatgcc tattcggaac agtaggaaaa
    23221 gaaccaatat aagcattatc aaaaacaata gaattttcag caaacttatc aatattagga
    23281 gtagaaatcc atctattccc ataacagccc aaaaaatccc ttcttaaagt atctgatata
    23341 acaataatca aattcatttt atccctcctt tttatatcct aatttttctg aaatttcatc
    23401 acaaactttt ttcaaataat ttccaaactc atcaatcttt tttttgtcta tcctgtacga
    23461 tggacctgct ataccaataa ttcctattag atttccatca taatcaaaaa taggtgcgga
    23521 tatccttgta acatctattc ttgcttcacc aatatcatat gcccatccat tttccctaac
    23581 tttttctatt tcttttttca atttcatttt atttgtgatt gttttttctg taattttttc
    23641 aagttttcta ttttttaaaa atttttcaaa aacataatta ttatatgcaa gaagaacttt
    23701 tccaggagca gatgcatgaa gtgttttata tcttgaacca atttgtgcaa caagttttat
    23761 tgattgtgga ctttctatct tatctatgta taatatctcc cctttatcag gtataatcaa
    23821 ttcaactgtt tcttttgctt tttcacagat tttttttaag tatggagttg ttatttctct
    23881 taaaggaatt tcagataaaa ttttttttgt aaaagttaaa aatccataac caagtttgta
    23941 ttttttatcc tcataaatta taaaatttct gcttatgaaa gtttttaaaa ttctgtaaag
    24001 ggttgttctg ggaattttaa gttttctctg taattctata aatgataaat aaccttctgc
    24061 ctctttcaac acttcaagaa catctattcc ttttgaaagt aaattttgtt ccatatttgg
    24121 aataattata ccatatttag aacaaagcaa aaattttttc aaaattcact attcataatt
    24181 ttaagaagaa aggactcgac caggcgaaat tgtcagaagt agttttaatt cttacataat
    24241 aaaaaacaaa gtcacccaga agttctgatt taagataatt ttttttatcc tcatcatctc
    24301 aaataaatgg ttaattgtta gagaaaagga ttccagtttt aaaacttctg aaatattttc
    24361 atcagggaaa ataatattat tatccatttt aatttttagg attaaagata agcatttttt
    24421 tcgtattctt caattaaggt cataaaatta tcatatttta caccataggc aattgaatgg
    24481 cttggtccaa ttataaatct taaattcttc tttttaaact tttcaataag gtctcttata
    24541 tttttcctga catcctccat tgtcccgctt ataagaattt ctgttgaaat accaccccaa
    24601 aaaataattc tatctccaaa tctttttgtt aaatattcta tatccatacc agcatttgtc
    24661 tgtaatgatt gataaacatc aattcccgct tcaataaaca tttccatcaa aggaatatta
    24721 ttaccgcagt tatgaagaaa aacatatggt acatactttt ttatattttc aacccttttc
    24781 tttaaaaatg gaaaacatac cttactaaaa atatcaggag aaataaaagg tccctttgtt
    24841 cctgccatat cctgttcaag taaaactcca tcctgtccct ttcttatgta aaatttatca
    24901 agaaaattct gtatttttac atttctttca tttactgctt ttataacttc tggttttaaa
    24961 gcatataaca tcaatcctgt ttccattcct cctataagtg taaaagcagt tatacctcct
    25021 gaagttcctg caataaattt ttcatcccta aaattttcaa tcagataatc aggtgcctga
    25081 aaaattgact ggtctggttc tttaaggtca gattcagaaa atttgaacat atcaggtgtg
    25141 tatgtatcta tatctatttt atttttatca ggattataaa tacatgaaat ctcatttgtt
    25201 gattcagaaa ttctataaat tcttccattt tcatctttca atgtgttttc atctattttt
    25261 tctatttttg gaggattgta attttcagta ggcaaaatac ctgcttcttt aaatgtcaat
    25321 atatcaacac aatctatttt tttaaaaaac tcaactgtat cttttttata actttcacaa
    25381 acttcatttc tctttccttc ccaaagtgca atctgtgatt ttactttatt ccttacatat
    25441 gttggccttc caataactct ttcaataata tcatagtcaa caacataaaa accaagtgga
    25501 attttatcta tatcctttct ttctattaat cttttaactc tttcttttga tgtcattcaa
    25561 ttattttaac ctttttcaaa tttttgtaaa tattgtaatt gcttcattaa aaactctaaa
    25621 aataattttt tgtttttctg tatcaaaaat tactcaataa tataaacttt aaaatcacga
    25681 ggagggactg aaatcttaaa ttctggactc tgtgatataa gttttaaatt tgaaatatca
    25741 aattttatag tttcaggcaa tttatgtaaa tcttttgtta attctttaat cctctcttta
    25801 ttttttacag ggtcctgatt atatagtttt tctgcttccc ttaaattatt ctcccactca
    25861 attaattttt catcactttc aaggtcataa attttaaatt ctttgttaag atttaaattt
    25921 ttcgtattta gtgtaatttt aacatcagtt ttgttttctt cagatttgtg aagattcatt
    25981 acctgaatta aaacttttcc ttttggttgt gtccatatac ttacaataaa tttatcatca
    26041 cttaccttaa catattcctc attatcccag aaaggaataa aattaacatc atctctatct
    26101 attccccatc cctgtcttaa aacaattaaa ggtctccctc tgactgctgt atgatattca
    26161 ggaacaacat catgcatcca gaaccaaccc actgtctgcc ttccaaaaaa tccccacgaa
    26221 agaccacttc ttcctacctg aaccatccac acaggtataa aaccaaattt ctgcgccctg
    26281 ccaagagttc ttaataataa agaggggatt gtctctataa tatcgcttcc tggaattgga
    26341 gttttatcct ctccaaacat tgctacatca gcaggtgaaa ttgaataagg tgacatataa
    26401 tttgttgaat gaacccagat aaaaggttct ctattatggt caataaataa ctgtctcatc
    26461 cttttcataa attctctctg gtgcattaat ggaatagatg gtcttatact tccatcttcc
    26521 ataattttcc cgtttccatt aaatacatta tagtcaggac catggtaaac ctcatcaaaa
    26581 taaaaacctt caacaaacca gtgtctgaac cataattcca tataaaaagc aaaaaaatct
    26641 ctataacttt caacaggtgt taaatgaaac caacttgaag ggataagtgt ccattcggca
    26701 ggaaaaaatg tggaaaaacc ttttgattta tatccttcaa agtacatact tgcattgaaa
    26761 aaataacatc ttttgacctt gtaaggattt gggtcttttt ttgccttatc ccagtttttg
    26821 ataaattcaa caccattttt atttttacca aggatctttt cagcattttt taaaacatct
    26881 ggatttgacc aatcaatata attttcagga agattagcaa gtatttcatc tggatttttt
    26941 ccttcctgat aaacacccat tgcctttgca agtttttcgc tatcatgcgg gtctattgga
    27001 caacctccgt gaccaggacc ataaaaaaag tttattttac tgtttgcaaa tggtctgaaa
    27061 tcaaggcatg catttctcca agaagaaggt agaggtttaa caggagaagc aattaaacca
    27121 aaatccgctg aaaaatcttt tatttcaccc ttttttctta caaaattaat cttcatttca
    27181 acaattttat cttttttcaa aatttcagca gttggaaaat ccttacccag tatccagtta
    27241 tggtcattat cagcaaacca gtgtattgca atttcatcat ctgttaaaca gatataagga
    27301 acaaaatcag caatttcgtc gcttttccat acacttttat tttccttccc tatctcacca
    27361 taggaaggac tccatcctcc tgcggttgaa aagtaatact ttgcataatt ttcatttaca
    27421 ggaattgaaa gatatattct atcaaaggaa attttttctt tttcaggaac attaacaagc
    27481 atcttataat aaatcatccc atcaaattcc atatatccat ctattttgaa atttatattt
    27541 cctattttat attcacctgt aaattcaact ctttcatcac ttttctttgt aattcttata
    27601 ggttttgaaa taattgcagg tattaatttt ccttccaatt cagcaaaaag tccaatattt
    27661 cctgcaagca tttctttacc ctgattcttt attctttcag gaaaaactcc tttaaattct
    27721 atctctcttc cccaaacttc aagaattaca ttatcttttt cttttatttt tatcttttca
    27781 aaaggtggag caactttaat atttttacca aaattgtttc caagccagac aaattctttc
    27841 tttaaatcct tatgctcaaa tgtatctatt ttttcttcaa gtacttttcc ttctttacta
    27901 agaaattttg tataaacttt atattttcca tattttaaat cttttggaag ccagatataa
    27961 ctttcagcac tatcatattc aaatttttca acttctttac ttacaataac cttatcaaga
    28021 ggtgaaacaa cttcaaatat aacacttttg agtttatcaa tatgaggata atcaagaata
    28081 tcacaccatg tataaagagc aagtgaaatt ggagcaaaat taacccttgt tttcatctgc
    28141 catttttctt cctcaacttt aatttctggt tttctttcct gctggtagag tgaataattc
    28201 catttattta aaaagagtgt tttaccagaa cttgcaattt taattaaaaa aatttgtttt
    28261 gtatttttct gaatttcagg caatatttca tctattttta tatttatctt atcctttgct
    28321 tttaatttaa aacttttatc ataaacaact gaatttcctg cttcaacatg aatattaaaa
    28381 ttttcttcct gattttctcc atttattatt tcaacttctg ttttcaattt attttcaaga
    28441 agtgatgata ggtcagttat ttttgaacag gtttctgtct gtgaaaatat aaattgtgca
    28501 tgtgcctgaa aatttttata ccatccatat gtatatggaa atgaacactg aggccatgca
    28561 actccaatca ttcctctcca tacattattc ttttttatat cctttattcc aaaactttca
    28621 aatggaatgg aaatttcaac catccatttt ttatctttat atgatgactt aacaatccag
    28681 tttccattcc atatagttct ttcaactcct aaggaaggaa tatgttcagc atcaaaaacg
    28741 gcatcataac cattcacaat catctgataa cacttaacat tcccttcagt aggtggtgtt
    28801 aaccatattt ccacctgagg attatcaaga taaacaggct catctcgtct tctataattt
    28861 tttgaaggtt tcctttcaag atttttataa acaatcccta cgaaaagatt atcattatta
    28921 tgcattagat aagcaaaagt gttaacacca atttcaggaa caattatccc accaaactgt
    28981 cctgttgcac ctacaaatcc tgtaataaca cacgcttttt cccatccctt ttcatcaaga
    29041 attccatcaa tcaaaggtgc tttttctgaa taagggatta aaatctgagg aataaattct
    29101 tcagaaaaaa caaatttgta aaagaaaaga gagaaaaaaa atatcaaaaa aatacttctt
    29161 ttcatttttc ctcctgttta ttttaatttt tactcccagg gatttattgc accttcttta
    29221 tagacctctt tatatatttt cccaacttct tcagtaaatt ttggcatcca ccatccatga
    29281 gtcatcaata tatctccttc cttaaccgct ttaacaattt catctcttct tttatcaatt
    29341 ggtccatcag caacatttat aatgctaaat gaatgaggaa aaagttttct tataatagga
    29401 ggagttgaaa caatcccacc tcttaattca gaatatggtg caatctgact ccatgatgca
    29461 gaacctttag gaaattcagg aattataaga acatcaggat gcttttttct cacttccctt
    29521 aaaacttctg tccagagtaa aatccattca aatttattaa cattttctcc aaccggtgca
    29581 aatattccat ttgtatccat ataaaataat gtacaacccc atctcttttt tgcatattca
    29641 attttccttg ataatctttc agcaatagga aaaaactgcc accatggtaa atccttcggc
    29701 catatttcag aaaaatcatc attaagtgga ttcctcatag gcccatggga ccctgttcca
    29761 tgcttccatt ttttatcatt ttcatcaaaa tatacttgtg ttggtcttat acaaacacca
    29821 gtccttaatc ctgcatccct gaaaatttta aaatattcgt ctgcaatctc attcatttca
    29881 ggaccaagta ttttcaacat ccttgggtct cctatatatg taattggatg tggattttct
    29941 tctccttcaa tattccaaac aatcattccc tgtgcattta tactttttaa aacattcaca
    30001 gaccttttag catgttcaat tgctatcttt ttaaattttt cttttccttc aggagaaaaa
    30061 acatctattg ttttatctgc aaaataacct cttggatttg tcgctgattt atgtccttcc
    30121 caactacttg caagcattat cattccaata ggccttctat cattccattc aagatgtggt
    30181 tgccagtaat ctctgaattt tttataaaca tcagaaacaa ttatatctgt atctgaatta
    30241 atatcagaaa atcttattga aaattcaaaa acagtatctt taccagaata tattcttggc
    30301 aatcccaaag gtggaataaa aacacctttt ggttctgctg caagaatacc tccttttata
    30361 taaaactccc attgattctg attttttctc aaaccataat ttaaaggtgg ataaactgat
    30421 gcatatgtta aaagaaattt atctgtaaaa ttttcctttc caaatttaac ttcaattaca
    30481 ccaggagatt caagcgtatc aagttcataa aaattaccac tttttctaac tactttatat
    30541 cccagtcccc accagaaatc aaaaggtgac tttatttcct gtttttcagg taaaataaaa
    30601 ttcaaaattc ctatatcaaa gtttgcaatt gttctttcac ttttatttga aatttttaaa
    30661 acaacatcaa aattattacc tttattatta tactgaaaac ttatacttcc ccacggataa
    30721 ttataaataa gtgttttttc tttttctttc cattctgtat ttaccggttt atccttaaat
    30781 ttctcaaaat tataaaccct ctctccattt tcatttttcc ctattttttc aaaaacaatt
    30841 tcttttaaat ctggctttcc ttcaattaca aaatcaatat ctttatatga aagattcaaa
    30901 accccttctt ttgtaaaact cacattaatc ttattctgag atgagcaact gaaattgaaa
    30961 aataaaagaa agataaaaa
//
LOCUS       DAOSAT010000010        30790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_1094_length_30790_cov_5.266374, whole genome shotgun sequence.
ACCESSION   DAOSAT010000010 DAOSAT010000000
VERSION     DAOSAT010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30790
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1094_length_30790_cov_5.266374"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PLF90_01610"
     CDS             <1..1082
                     /locus_tag="PLF90_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOK56014.1"
                     /translation="GLKIGKGDEVICPSITYWASCLPVLSLGGTVVFADIDEDSLCID
                     PSDIEKHITEKTKAIIVVHYAGYPADMDKIMSIANKYNLKVLEDCSHSHWCFYKGKLT
                     GTFGDVSAFSLMTGKSFPIGEGGIMLTDDLEIYERAIIFGHYERHSEITIDYLKNGTG
                     IPWGGYKYRIHQLSSAVGIVFIKIFKEIFEEIDKAMNYFCDLLDTLPGIKTHRPPKNS
                     GLTKGGWYFPLAHYNKEELNGLSITRFCEALREEGISVYPGCNKPLHMHPVFQNIDIY
                     NDGKPTIIRNSEKDIRSKKGSLPVSENIGKKVFSIPRFTKFNKKVIEKYFSGFEKVVK
                     NHKELLKDDPGDPPEIGNWSLTFRI"
     gene            1095..2333
                     /locus_tag="PLF90_01615"
     CDS             1095..2333
                     /locus_tag="PLF90_01615"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOK56015.1"
                     /translation="MDKLVIDGGKPLRKKPFPQRIMFDEKEVKLINEMMKKATKENTA
                     KYLDRYAGSEVDTYEKEFAEFFGTKYATATSSGTAAIHSAIASLDLEPGDKIITTPIT
                     DPGTVMPILFQGLIPVFADVDFETLNLTAKTIEEKIDKKTKAVIVVHLAGVPCEMDKI
                     IEVARKYNLKIIEDCAQSHASIYKNKYVGTFGDMGCFSLMSGKHMTSGGQGGMVITDN
                     EEYYWRCKRFADRGKPFGIENPEGNVSLGLNYRMTEIEAAIGRVQLKKLKKIAEKRFK
                     IVEELRKGFSNLKGFSLWKKPENSKPNYWFCFIRVEEEKFKYDKWKLAEILIKEGLPA
                     GAHYVIQIYENPFIKARKTFGSSGYPWKPYSPNIEYKCPIAEKALNSHITLYIHECWG
                     KKEIEDTIKIFEKVERFCLK"
     gene            2431..3477
                     /locus_tag="PLF90_01620"
     CDS             2431..3477
                     /locus_tag="PLF90_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOK56016.1"
                     /translation="MPNRLDSPQITDNFIEKWAKRIKPELEFNCKTEEEFYDWKEKLI
                     KKIKELLGEFPEQADLNPEKISEEEREKYIREKWIIQTEKDCFMPLYILIPKGDKKKY
                     PAILCCHGHGPYGKDPVAGVHNNNPEIINNIKIHNYNYGEQMAEKGFVTIVPDWRSFG
                     ERIGYHNIFQPRDICNIHFLQHLICGRTLLGANIFDGMRAIDFLLTRDYVDKENIGCM
                     GLSFGGTMATYLTLLDNRIKAGDIICYATTTLHYAIERPNFCGSQIVPFLYKYADVGD
                     VIAAICPKPLLIESGANDTCFWIHSAKKAHEKVKKAYKVAGVSGDLWIEIFPGEHAFS
                     GKMAFKFFEKYLKK"
     gene            3474..4439
                     /locus_tag="PLF90_01625"
     CDS             3474..4439
                     /locus_tag="PLF90_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK56017.1"
                     /translation="MRNYILKRLLQFIPLLIGMTFISFFIIQLAPGDYFTQLKMNPEI
                     SPETIENMRKLFALDKPIIIQYFYWLKNIFTFNLGESFSYHVPVSFLIKVRLKNTIAL
                     SIFSIFFTWVIAIPLGIFGAIKDKKWQERILSFLAYIGISLPSFFIAMLVIFFIAKTA
                     VLPLGGTTSIFYENASNIEKFIDYLKHLFVPGMILTISGIGSLYRLMKNNFLETLNSP
                     YIFTAYAKGIPTYKIYFKHALRNAINPMIIIFGYELSGILSGAGLVEIITGWPGMGRL
                     VLEGVLSSDLYLVMASLLIGGILLILGNLFADILIALVDPRVRLR"
     gene            4436..5491
                     /locus_tag="PLF90_01630"
     CDS             4436..5491
                     /locus_tag="PLF90_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK56018.1"
                     /translation="MKSILKRLKNKNFFSYCSFWIIIFLYFLAIMADFISPYPYNKQF
                     RDYPLCPPTRIHFFDHNKNFHLRPFVYEIKLVNPALNQYEIVKEKICPIYFFVKGEKR
                     KILGIFNSERYLFGTKEGKIFLFGTDMHGRDIFSRILYGARISLSIGIIGVSISFFFG
                     FLIGGISGYFGGKIDNILMRLVEIFMMFPAFYLMLSLRAVFPLNLSSFQIYLLIVIIL
                     SLIGWAGLARIIRGMVLSIKEEEYVLAARALGVSTFKIIFKHIIPNTFSYSITAITLS
                     IPGYILGESTLSLLGLGIQEPYPSWGNMLSVCIGNLRVLSSAPWMLTPGFFIFLTIIC
                     FNFLGDGLRDVLDPKNW"
     gene            5493..6437
                     /locus_tag="PLF90_01635"
     CDS             5493..6437
                     /locus_tag="PLF90_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK56019.1"
                     /translation="MEKILTVKNIFVGNEEIKILRNINFEVRKKEIVSIVGESGCGKS
                     MTLRAIIKLLPKNLKILNGEIFFENLKITEYNEMEKIRGKRIGMIFQEPSSYLNPLFT
                     IGNQIEEGIKENIGKKEKKEKIVKILKEIGLSPDVYYSYPHQLSGGMQQRAMIAMALI
                     NNPDLLLADEPTTALDVTTASGIIKLLKDLVSIYNLSIIFVTHDISLAKYISDKIIVM
                     YAGIIVEKGNSEQVYNNPLHPYTQKLINCLPEKYSKRERIKTIEGEVPSFKNLPYGCP
                     FSTRCEYKKRICEEKIPGEIVIENRIVRCFKYGNIVES"
     gene            6415..7173
                     /locus_tag="PLF90_01640"
     CDS             6415..7173
                     /locus_tag="PLF90_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK56020.1"
                     /translation="MEILWKVENLTKEYEVKSEFKKIRINALKNVNLEIFKGEVFGLA
                     GESGSGKTTFGKVLIRLIEPDDGKIIYKDIDITRMPEKILKKYRKNFQIIFQDPYKSL
                     NPRIPCGLTVMEGITGSKKEKFEKSIELLNLVGLKKERFYDYPHQFSGGERQRIAIAR
                     ALSTNPEFIVCDEPTSNLDVSIQGQILNLFIDLKEKFNLTYLFITHNLKILRFISDRI
                     GIMYRGEIVEIGKTEEIFTNPKHPYTEKLIKSEI"
     gene            complement(7206..8396)
                     /locus_tag="PLF90_01645"
     CDS             complement(7206..8396)
                     /locus_tag="PLF90_01645"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK56021.1"
                     /translation="MVFRGLIRNKEIKINEIEIISNREWPLYTILLPIYKEKEIFNQL
                     YMAMENIDYPKDKLDIILLIEEDDLEIKEYLKNYKLPSHWRIIEVPDFKPKTKGKALN
                     FGLKKAKGVYLVIYDAEDIPEKDQLKKAVIGFRKVSDNVVCLQSKLNYYNPNQNLLTK
                     WFTAEYSSWFDLYLPGIDSIEAPIPLGGTSNHFKADILKELKGWDPFNVTEDCDLGIR
                     IFMSGYRTKVLDTTTWEEANSELKNWIKQRSRWVKGYIQTYFVHLRNPFKLIRKIGFK
                     NFLHFNLIVGGNFFVLCFNPIAWVFLLIWLFSPHRIFFPNIFFMIVTPILLLGNIIFV
                     LINILGVLKRKWYKLVIPAILSPFYWILMSIGALKGFFQFFRRPHYWEKTQHALFITS
                     IEQI"
     gene            complement(8590..10893)
                     /locus_tag="PLF90_01650"
     CDS             complement(8590..10893)
                     /locus_tag="PLF90_01650"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOK56022.1"
                     /translation="MKKFLLIFFILSSFIFSQYSSTGSVKKEDIFFDFEEDIQIWKIT
                     PIKNNCIKDITFSDEKSLSGKRSLVFKIDDSGEGSAEKEFFKDLSIYKNIIFNIYIPA
                     SAPEDIKICFFLQDNEWLWYQTPLFILKKEQWNKITINVEPGSTFWENVGHPQPWSEK
                     TICNIRKIGIKIFLNTKFTGSIYIDEIKGSFKVFPEISISKKKLLQFEKFEISFFLPQ
                     KYSNPFDPEEISIDGVFIDPENNTFVVPGFYYQEYERSLENGEEVLTPVGYLYWKIRF
                     TPEKPGRYKFYVKIREYDKEVTTDINYFIVEPCDTKNKFVKVSNIDNRFFSYKSGEFF
                     YPIGFNIRSPTDERYAQLMGKRVDSDSGTFYYENIFKKMKENGINFTEVWMASWFAAL
                     EWKENRPGYRGVSYYNLRNAWKMDKVIEFAELNDIYVQLVIINHGQLSTWCDQEWQDN
                     PYNIKNGGFLKSPNEFFTDERAKKFTKNKLRYIVARWGYSPYIFSWEIINEINLIGAF
                     HNFYLRDEITEWYKEMKEYLKEIDPFQHMVTAHYTILVNNKILSDIIDYTITNGYYNF
                     SQTNLPTFFRSIYNFNSIFKKPTFISEYGGTPMGSSFENLKRDIIMGLWFSFHLPFAG
                     TPLLWWHRFIDEFNLYYIYKIFSEYTKGVDRLKFPLESENVIIDGAGKNQIFTVATGN
                     RFFSSVFLYDYSVIRNVERRDFPEYNNMICFLKDKRTGKFKVEFYDPEKGKIDEKEIL
                     SDEKGNLKVEIPPFKKWIALKVNFYGE"
     gene            complement(10893..11906)
                     /locus_tag="PLF90_01655"
     CDS             complement(10893..11906)
                     /locus_tag="PLF90_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK56023.1"
                     /translation="MVRVGIIGAGFMGNMHALCYSQLPDVRIAGIADIRGDKAKSLAT
                     KFKTIPYFDPEQLIKKEDITLIDICLPTFLHKEYVIKAAEAGKDVICEKPIALTVEDA
                     DEMIKTCERNKVRFAVAQVIRFWSEYKYLKDIYESGKYGKLLTISMRRVSPLPTWGWQ
                     DWLLDQEKSGGALIDLHIHDTDYLLYLVNEKPQKIYSKIAKKNGLDAHVFTIFTFKNG
                     LIAEIEGGFDFPPSFQFEMSFTAKFEKATIDFSSNKKPTVTVYELSGKVERPSFEKIK
                     ANGVEGNIDDLGGYFYELRYFVDHIIHNKPFQVVTPEDARNSLEIVLKEKESALKGGE
                     IEL"
     gene            complement(11985..13358)
                     /gene="mgtE"
                     /locus_tag="PLF90_01660"
     CDS             complement(11985..13358)
                     /gene="mgtE"
                     /locus_tag="PLF90_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOK56024.1"
                     /translation="MADEKFVKTLLKNEIIELIENKDWYNLKEALVEWPSPDIAELIE
                     ELDEECRIIVFRLLPKKLSAEVFDEVNPIVKENLISKLTDEHIKNILSELPPDERTDL
                     FEELPGKVTQRLLNLLSPADRKEALQLLGYPENSVGRLMTPDYVAIKKDWTIEKAFSY
                     IREFGKQAETINVVYVVDENWKLLDDISLAKLILSEPNETVEKIMDFNFIAIEANKDQ
                     EEASNLMRKYDLIVLPVIDEDGVLLGIVTVDDVLNVVEQEHTEDMTKMFAITPETAGV
                     ELITDLLKTPIKNLYKSRVTWLIFLLFMDLFTGGIIKRFEDTIAKYIVLVTFLPVLVD
                     TAGNAGSQSATLIIRSMALGKIRMKDWLKMFGREILVALMLGLTMGLGISFMGIIRGG
                     LSICKVVVVSMVVNVIAGCLMGMALPFIFTKLKKDPATASAPLITTLADILGTGIYLT
                     IAMIMLK"
     gene            13439..15442
                     /gene="uvrB"
                     /locus_tag="PLF90_01665"
     CDS             13439..15442
                     /gene="uvrB"
                     /locus_tag="PLF90_01665"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOK56025.1"
                     /translation="MEKFNLVSNFKPSGDQPKAIEKLVKNLEKREKHQILLGVTGSGK
                     TFTMANVIAAVNKPTLVISHNKTLAAQLYSEFREFFPENKVRYFVSYYNYYQPEAYIP
                     ETDTYIEKDASINEDIDRLRLAATSAILSSRDVIIVASVSCIYPIGSPEDHKAMMLHL
                     SKNQIINRRYIIERLIEIQYERNDFDLTRGKFRVRGNTIEIIPSYEEIGVRIWIIGDK
                     VVKIQRFEPLTGNVISEEEEIYIYPAKHFVTPKDKLERAIESIKKELEERVEYFKKNG
                     KLLEAERLETRTNYDLEMLSEVGYCHGIENYSRHLAGRLPGERPEVLLDYFPKDYLIF
                     IDESHVTVPQIRGMYCGDRSRKETLVEFGFRLPSALDNRPLKFEEFEKMVNQVIYVSA
                     TPGKYELKKCGVERIGEPSPLIAEQIIRPTGLVDPPIIVKPTANQINDLIGRIKERVS
                     KNQRVLVITISKHLAEEIADYLKEFNIKVQYIHYEIDTLERVEILKGLREAKFDVLVG
                     INLLREGLDLPEVSLVAILDADKEGFLRSETALIQISGRAARSLDGEVIMYADKITDA
                     MKSAIDETERRRKLQMEYNMTHNITPKSIEKPIYKSLVEILGGKSRIKEKEEKLKEEE
                     YYGKDLVKIIKQLERKMMAAAKSLDFETAIYYRDKIKKLKEEK"
     gene            15443..16546
                     /locus_tag="PLF90_01670"
     CDS             15443..16546
                     /locus_tag="PLF90_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56026.1"
                     /translation="MDIGNMIKKMVEKVESYSEKIKENKRKQESVWNNKKTEIPIITG
                     KIDNEVKNYPDFNMKESFYDPEKMLYMQLKGIVNIVSSYSDACPSVRFNFGTGFIPSI
                     FGLESEIFEDKMPWLKKHLSKEEIKRLKFDDFENIEEMGLMKKIPVYLKTYKKYLSKN
                     IKIYLPDTQGPFDIAHLIRGDEIFTDIYDDFEFFKYILEISTYIYIKATEKLKNLIGE
                     NITESYHSGSYYIKNGGIRICEDSTTLVSPKHLEVILTYTQKCLKYFGGGWIHFCGKA
                     EHLFDLVIEIPEVSSINFGNPEKYDFKYVFKKLNEKNKVYLGSIPREKDEEWKKYLKR
                     VYEMCEGKNLIFSPTLRENEKIEEIYEYWGSLQ"
     gene            16543..17442
                     /locus_tag="PLF90_01675"
     CDS             16543..17442
                     /locus_tag="PLF90_01675"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOK56027.1"
                     /translation="MSGLFKISKPDPLEYEKRGDFKGLIKLLNYKDDPYIRWKSAEIL
                     GKYKVREAVPYLIKKIDDPEWRVRAKIIEALGIIGDQSSIEPLLRHIKDEYPEVRCSV
                     CYALGRLKDKRATIKLIELIKDKNRFVQKAAIEAIGEIGDESAFDELIKIARNKPPEI
                     KVLIAETLGKIGKNLVKRMINLLEKTEDKDIKWIIIKALGDIGNKEAGPILTKNLKDI
                     SYEIREVSAESLGKIKYEDAVDILIEMLNDNEWVVRKKVIEALEKIGNKKALPYLYEL
                     EKIEKVEDVRVKIRETIKTLNTQ"
     gene            17482..18258
                     /locus_tag="PLF90_01680"
     CDS             17482..18258
                     /locus_tag="PLF90_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOK56028.1"
                     /translation="MKIYIVTDLEGATGVFKFSQTRERGPEFYEAMKFLMGDIAAVCE
                     GLKEAGVKEIYVIDGHDGGNNFIPELMIPGVRYITGRPRPGVFYGLDETFDGVILLGY
                     HAMNGTPDGVLNHTQSSIAELKYYYDGIERGEIYQSAVIAGHFNVPVILVTGDEAACR
                     EAKKTLGENLPTVAVKKGISREAAILIPPEETKILLKEGAKKAIKMLPKLKPYKIKLP
                     VKLKIRKIGPEGTSPDNPYFTEKEVIVKSALDIITGSKNP"
     gene            complement(18300..18869)
                     /locus_tag="PLF90_01685"
     CDS             complement(18300..18869)
                     /locus_tag="PLF90_01685"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOK56029.1"
                     /translation="MNQKQKKAEIRKEIRKEREILEFKDWEEKSFKIQQKFLLSDFYK
                     NSKVIFTYYHFDREVRTDLIIKKGIEDKKIICLPYINWKNKILIPSEIKSTEEIIEIK
                     GIPGPKFLRPVEIENIDIVIVPGVVFDIYKNRIGMGGGFYDRFLKNIPSETKKISLAF
                     EFQIFDEKLPVDENDIKIDIIITEKRIIT"
     gene            complement(18805..20175)
                     /locus_tag="PLF90_01690"
     CDS             complement(18805..20175)
                     /locus_tag="PLF90_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOK56030.1"
                     /translation="MNGLFKSEVEKKFENLPLSIRVRPEKLEDFVGQKHLLDEGKPLR
                     KIIESDRIPSLVFYGPPGCGKTALAMIIANMTKKKFVSLNAVTATVSDVREVLKEARE
                     RQRLEKRGTILFLDEISHFNKLQQDALLKDVEEGTITLICATIHNPYFYINTPLLSRS
                     LVFEFKPLSEEEIEEIIKRALRSKKGLGNYKIKITKEAIEFIGKKSQGDARKALNTFE
                     FCILTSEIEKDGYIIIDLNKVKEFVDKYFIYDRKHDAHYDTISAFIKSMRGSDPDSAL
                     YYLAKMLVSGEDPRFIARRMLIFASEDIGNADPQALILANSCYQAVEVVGMPEAKIIL
                     AQTVIYLSTSPKCNSSYLAIEEAMKEVESEKVEEVPDHLKGTGYKGAEKLGRGIGYKY
                     PHDYPSHYVIQKYREGTKKFYFPLDIGYESKIKKFLEDIEKLKNESKTKEGRNKEGDK
                     KGKGDT"
     gene            complement(20177..20740)
                     /locus_tag="PLF90_01695"
     CDS             complement(20177..20740)
                     /locus_tag="PLF90_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HOK56031.1"
                     /translation="MTETYTELINKYFEELKGLIKKKKLKEDFIYGENEIELTYLNQV
                     IKIDILSMKFNKDINDKEKVIILRYLCKFPYKKSDELITFKSLPEGNFYFPSIYSRIY
                     LPLIKKYGKEPEKFVGKSIEIGAERFNDFSVKFNIFSDVFFIFDLIPEDDEFPADLRI
                     LFNKTTSEIFEIEDIAIIGEIIVSKIT"
     gene            complement(20742..21617)
                     /locus_tag="PLF90_01700"
     CDS             complement(20742..21617)
                     /locus_tag="PLF90_01700"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOK56032.1"
                     /translation="MFLIGENINIMNKKIKGAINERKKEIIQKVAIESKNNGMDYLDL
                     NIGPARKDGPFLMEWIIKTVREVCDLPLSLDTTNIEALKVGLEMEGEKAIINSIQATE
                     ERMDELLPLVKKYNCKFIGLLLGKEGMPRDENERGAIAANFLAKIDEYEISHDKVYFD
                     PIVLPVRFQQEQVVSVIEFMKMFNDLFSDCNSTCGLSNVSNGALNELRGLINRTYLVM
                     LWKYGMNSAIVDAYDKELISLVRGERDDIKNLIWKAMDEEIDLTGLNEEKRNYVKTVN
                     ILLGKSIYSDSYLKL"
     gene            complement(21633..22961)
                     /gene="acsC"
                     /locus_tag="PLF90_01705"
     CDS             complement(21633..22961)
                     /gene="acsC"
                     /locus_tag="PLF90_01705"
                     /inference="COORDINATES: protein motif:HMM:NF003195.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HOK56033.1"
                     /translation="MALKPLDIFKFLPKTNCKECGFPTCLAFAMQIAIGKAELSQCPH
                     ISEEAKKQLSAVSAPPIKTITVGNKEKVNLGGETVLYRHEKTFYNPTGIGVLIKESME
                     EQEINREIKNITDFNYSRVGIELKAEFIGIKDEGKGKFIDIVKRCYETGKNLILMSDN
                     SETIKNSLSICKDVRPIIFSEKISEEILNIAKEFNLPVIVRGETIEEITSNVKTLQDK
                     GIEEIIIFPSNNEISDLFKNVIFIRRGAILKKIKEFGYPILTIPSFLTDDYLKEGVYA
                     SIFIAKYGSIVLLSKLRGEVLFPLLIERLNIFTDPQRPLTTPEGIYEIGKPDENSPVF
                     VTTNFSLTYFIVSGEIENSRIPAYLLIQDTEGLSVLTSWAADKFNAETIANLVKKTKI
                     GERLNKKVLIIPGVVAQIYGELEEELPDWKILLGPREAAHIPSFLKNVEI"
     gene            complement(22981..23730)
                     /locus_tag="PLF90_01710"
     CDS             complement(22981..23730)
                     /locus_tag="PLF90_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK56034.1"
                     /translation="MKPLKIGVAGKGGTGKTTLTSLIILSLLKMNKKPILAIDADPNS
                     NLAENLGIPQPKSLVEIVDEIEKIKNNLPYGMEKSKYLEIRIQEAIKEEKGFDLLVMG
                     KTEGPGCYCYANNLLREWMEKIEKNYLFIVMDNEAGMEHISRRTSGDLDILFIVSLCD
                     KISLRTAENIYKMINSLELSIKKTYLVLNEFMKKNLNEVKTLIPIAFTIPFDEEIYSL
                     IEENKGLLNLSENSSAYSEVKKFLNFLLSNL"
     gene            complement(23727..25589)
                     /locus_tag="PLF90_01715"
     CDS             complement(23727..25589)
                     /locus_tag="PLF90_01715"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOK56035.1"
                     /translation="MKEYFILTVKPLDKKIPIRKGTDILSALIKGNIFITSSCGGKGI
                     CGRCKVKILKGDFYSEPTGKITEEEKRNNIYLACRTTILDNMEIELLPETVIPEKIER
                     EFEKGEEFEHIRIDVKELFSFSPLIKKIFLRLPSPNFSDTLSDYERVKRNLRKYINKE
                     KFRIDISNLRKLPNLIRSSFWEITVTLIENDDMVEILNIEPGDTSKRNYGIAVDIGTT
                     TISASLIDINKGDVLKTEITFNRQASFGDDIISRIVYAEKKDGLENLHHVVTETINDM
                     IINLSKKCGISINDITGISISGNMTMVHLLLKINPSFLRKEPYIPIANEFPLIKSAEI
                     GIKINPSGICEIVPGGFPYVGGDITSGIIVSGIYKEDVLSIFLDIGTNGEIVVGNKDF
                     LVCCAASAGPCFEGSGVKNGVRAIKGAIQKVKISNGDVFFETIGGVEPIGICGSGYIE
                     LLSELYKNGIIDRNGNFIKENERIIENEGIKEFIVAENKKGEKIVITQADIDNLIRAK
                     GAIYSAISTVLKTLGIKIEDVKKFYIAGGFGKHLNIKKSIEIGLFPNLPEERFKFLGN
                     TSLLGAESFILSEKFRKLSKEIIKKLTYIDLSRSPIYMEEYLKALFIPHTDFEK"
     gene            complement(25586..26656)
                     /locus_tag="PLF90_01720"
     CDS             complement(25586..26656)
                     /locus_tag="PLF90_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK56036.1"
                     /translation="MPKNIFIAATRQNDRKTVISIGLFLVLKKYVDKIGFIKPVGQKY
                     LVIDGKKVDKDAVLLKRICKLENELEDMNPIAVEEGFTRNYLDNPNKDELVEKIKKSY
                     EKISEDKEMVIIEGTGHAGVGSIFDLNNAEVARILNSPVILISIGGIGKPADEISLNL
                     SLFKEKNVSIKGVIINKVQEEKKDILEKYLNKFFQKIDLKLLGLIPYVPYLTQPNLFQ
                     ICDAVNGKIITGWNKMREKIKNVIVGAMTPRHALDYFKPDSLLITPGDREDIILAALS
                     TSMQNLIKGIVLTGGIYPHKTILELIKKYDFPTIVAEEETYIVASKINELVVKLTEND
                     LDKIEIAKKIIEENVDIEEILK"
     gene            26715..27482
                     /locus_tag="PLF90_01725"
     CDS             26715..27482
                     /locus_tag="PLF90_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK56037.1"
                     /translation="MKIAISGKGGTGKTTISACISKVYLEKGKRVILIDADPDSNLKS
                     TLNIKKEIIPLIELKEIIAERTGTKPGERAIIFKMNPEVDDIPERFFYRNGNLLVGIM
                     GTVRGGGLGCMCPENAFLKAVIRNLILKRDDVVVLDMEAGIEHLGRGTTEGIDWLLII
                     TEPTEKSIETTERIEKLAKEIKIKKTGVIGNKIESEIEKNFIIEKLKNSEILGYIPYF
                     PEMKKGEIEGKTPWESAPIILNYIKEIVAKMEAKNGR"
     gene            27472..27642
                     /locus_tag="PLF90_01730"
     CDS             27472..27642
                     /locus_tag="PLF90_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56038.1"
                     /translation="MEDKKVLGETYEEGKPKKSLKWKKFENKDEMIKFIKVSERYWFG
                     EGYGSEKRKTPA"
     gene            27655..28593
                     /locus_tag="PLF90_01735"
     CDS             27655..28593
                     /locus_tag="PLF90_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HOK56039.1"
                     /translation="MDELKLNYTGKIREIEIGIGEKKIKVGGQNGFNFHYFECEFPNE
                     PIISFEVWDSQPEEWPENCLSPYKDVINDPLSWAKKCVNEYGAKCLTIFLNSTDPNGM
                     NKSASDVRPIVEKIVNGIDVPLIFWGSGNVEKDIEVLKMVCEVASGKNIVVGPVVDKN
                     YKQIGASAIAYKQVVIASTPIDINLAKQLNILLLELGVPENKIIMDPTVGSLGYGIEY
                     TYSVMERDRIAGLLQGDDKLAFPIYCYLSKEVWKIKEVSIPENMDIKLGKEKERGIMF
                     EAITATLLLLAGADIIVIRHPETVKLISELMEEMKS"
     gene            28606..30492
                     /gene="cooS"
                     /locus_tag="PLF90_01740"
     CDS             28606..30492
                     /gene="cooS"
                     /locus_tag="PLF90_01740"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HOK56040.1"
                     /translation="MAELIETKFSTVEERKKRQQPHCKFGLLEICCRLCSMGPCRITE
                     KSPKGVCGANEDTIVARNFLRKIAVGASAHSDHGRAVAETLMKVARNEAPGYEIKDEF
                     KLIKLALEFDIDVKNKKTEEIALEVGETCLSQFGQQEGELIFTKRAPLKRQKIWKELG
                     ITPRGIDREVVESLHRTHMGTDQDYLNLMKQAQRCALSDGWGGSMIATDLQDVLFGTP
                     EPIIGKVNFGVLKEDYVNIIVHGHEPLLSEMIVIAAEDPELLIQAKSKGAKGINIGGI
                     CCTANEILMRHGISIIGNFLNQEMAITTGAIEVMVVDVQCIMASLPEIASCFHTKIIT
                     TSEKAKIPGAIHIQFTEENALQKAKEIIKMAIENFENRKRENVVIPESQMDLVAGFSY
                     ESIYYILGGRFRATYRPLNDNIINGKIRGIAGVVGCNNPKVAHDEIHIKLVSKLIEND
                     ILVLQTGCSAIVCAKAGFLDSVKGLNYAGKGLREVCEAVGIPPVIHMGSCVDNSRILM
                     AATCVVKEGGLGDDISELPAAGAAPEWMSEKALSIGQYFVSSGVFVVFGVTWPTFGSK
                     NLTKYLFEDIEKEVGGKWAFESDPDKMAELMIKHIDEKRKKLGIDKARERVLYDMAMR
                     RELE"
     gene            30489..>30790
                     /locus_tag="PLF90_01745"
     CDS             30489..>30790
                     /locus_tag="PLF90_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008727913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5674 family protein"
                     /protein_id="HOK56041.1"
                     /translation="MIRMVDKNKKIKLSKLKDMAEKMFDNFVKCVVDVESEIMVVGGE
                     LHTDEEALLLETGSKQENLWGINIYPEKKDDEFIEFDSIINIRPSFGNKSREVENP"
BASE COUNT        11081 a         4514 c         4671 g        10524 t
ORIGIN      
        1 ttggattaaa aattggaaaa ggcgatgaag taatctgtcc ttcaataaca tactgggctt
       61 catgtcttcc tgtactatct ctcggtggaa ctgttgtttt cgctgatata gatgaagata
      121 gtttatgtat agacccatct gatatagaaa aacatattac cgaaaaaaca aaagcaatta
      181 ttgttgtcca ttatgcaggt tatcctgcgg atatggataa aattatgtca attgcaaata
      241 aatataatct aaaagtcctt gaagattgct ctcattcaca ctggtgtttt tataaaggca
      301 aattaacagg aacttttgga gatgtttctg ctttttcatt aatgacagga aaatcatttc
      361 ctattggaga aggtggaatt atgttaacag acgaccttga gatttacgaa agagcgatta
      421 tatttggaca ttatgaaaga cattctgaaa ttacaattga ttacttaaaa aatggtacag
      481 gaattccgtg gggaggttac aaatacagaa ttcatcaact ttcttcagct gtgggcattg
      541 tatttattaa gattttcaaa gaaatttttg aagaaataga taaggcaatg aactacttct
      601 gtgatttact tgatacactt ccaggaataa aaacacacag accacctaaa aattcaggat
      661 taacaaaagg tggatggtat ttccctcttg cacattataa taaggaagaa cttaatggac
      721 tttcaataac aagattctgc gaagcattaa gagaagaagg tattagtgtt tatcctggat
      781 gtaataaacc acttcatatg catcctgttt ttcagaatat tgacatttat aatgatggaa
      841 aacctacaat aataagaaac tctgaaaaag atataagaag taaaaaagga agtttacctg
      901 tatcagaaaa tattggtaaa aaagttttca gtattccaag atttacaaaa tttaataaaa
      961 aagtaattga aaaatatttt tcaggttttg aaaaagttgt taaaaatcat aaagaattat
     1021 taaaagatga ccctggagac ccacctgaaa tcggaaactg gagtttaaca ttcagaattt
     1081 aaaaaggaga aaaaatggat aaacttgtta tagatggagg aaaacctttg agaaaaaaac
     1141 catttcctca aagaattatg tttgatgaaa aagaggttaa acttataaat gaaatgatga
     1201 aaaaagcaac aaaagaaaat acagcaaaat accttgatag atatgctgga agtgaggtag
     1261 atacatatga gaaagaattt gcagaatttt ttggaacaaa gtatgcaact gcaacaagtt
     1321 caggtactgc tgctattcat tctgcaattg catcacttga tttagaaccc ggggataaga
     1381 taataacaac tccaataaca gaccctggaa ctgttatgcc tattttattt caaggactta
     1441 ttcctgtatt tgctgatgtt gattttgaaa cattgaattt aactgcaaaa acaatagaag
     1501 aaaaaataga taaaaaaaca aaagcagtta tagttgtcca tcttgcaggt gttccctgtg
     1561 aaatggataa aattattgaa gttgcaagaa aatacaattt aaagattatt gaagattgtg
     1621 cacagagtca tgcttcaatc tataaaaata aatatgtagg aacttttgga gatatgggtt
     1681 gttttagttt aatgagtgga aaacatatga caagtggagg tcaaggtgga atggtaataa
     1741 ctgataatga agaatattac tggagatgta aaagatttgc agatagaggg aaaccatttg
     1801 gaatagaaaa tccagaaggt aatgtatctc ttggtttaaa ttacagaatg acagaaattg
     1861 aagcagcaat aggaagagta caattaaaaa aattgaaaaa aattgctgaa aaaagattca
     1921 aaatagttga agaattaaga aaaggatttt caaatttaaa aggattttct ttatggaaaa
     1981 aacccgaaaa ttcaaaacca aattactggt tctgttttat tagagttgaa gaagaaaaat
     2041 ttaaatatga taaatggaaa ctcgcagaaa ttttaattaa agaaggatta cctgctggag
     2101 cacattatgt tattcaaatc tatgaaaatc catttataaa agcgagaaaa acatttggaa
     2161 gttcaggcta tccatggaaa ccttactctc ccaatattga atataaatgt ccgattgcag
     2221 aaaaagcact gaattctcat attacactgt atatccatga atgctggggt aaaaaagaaa
     2281 tagaagatac aataaaaata tttgaaaaag ttgaaagatt ttgcctcaag taaatcttaa
     2341 acctcttgat tatcaaagat tcccatatcc cacctttaag gtgggcaaaa agggaaaatg
     2401 tgatataata aaaaacacaa ggaggaaaaa atgccaaaca gattagattc tcctcagatt
     2461 acagataatt ttatagaaaa gtgggcaaag agaataaaac cagaacttga atttaactgt
     2521 aaaacagagg aagaatttta tgactggaaa gaaaaactta taaaaaagat aaaggaactt
     2581 cttggagaat ttcctgaaca agcagattta aatccggaga aaatatccga agaggaaaga
     2641 gaaaaatata taagggagaa atggataatt caaacagaaa aagattgttt tatgcccctt
     2701 tatattctta tccctaaagg agataaaaag aagtatcctg caattttatg ctgtcatgga
     2761 catggacctt atggtaaaga ccctgttgct ggagttcata ataataatcc tgaaataata
     2821 aataatataa aaatccataa ttacaattat ggagaacaga tggctgaaaa gggttttgtg
     2881 acaattgttc ctgactggag aagtttcggg gaaaggattg gataccataa tatttttcag
     2941 ccaagagata tctgtaatat tcatttttta caacatttaa tctgtggcag aacgcttctt
     3001 ggagcaaata tttttgatgg tatgagagca attgattttt tattaacaag ggattatgtt
     3061 gataaggaaa acattggttg tatgggatta tcctttggag gaacaatggc gacatattta
     3121 acattgcttg acaatagaat taaagcagga gatataatat gttatgcaac aacaacttta
     3181 cattatgcga tagaaagacc gaatttctgt ggaagtcaaa tagttccttt tctatataaa
     3241 tatgctgatg tcggagatgt aatcgctgca atttgtccta aaccactttt aattgaatca
     3301 ggagcaaatg atacatgttt ctggatccat tcagcaaaaa aggcacatga aaaagtaaaa
     3361 aaagcgtata aagtagcagg tgtaagtggc gatttatgga ttgagatatt cccgggagaa
     3421 catgcttttt caggtaaaat ggcttttaaa ttttttgaaa aatatcttaa aaaatgagaa
     3481 attacatttt aaaacgacta ttacaattta tccccttact tattggaatg acttttatat
     3541 ccttttttat aattcaactt gctcccggtg attatttcac gcaattaaaa atgaatccag
     3601 aaatatcacc tgaaactatt gaaaatatgc ggaaattatt tgctcttgat aaacccataa
     3661 taattcagta tttttactgg ttaaaaaata tttttacatt taatcttgga gaatcatttt
     3721 catatcatgt tcctgtttcg tttttaataa aagttcgact gaaaaacaca atcgctcttt
     3781 caattttttc aatttttttt acatgggtca tcgcaatacc tttaggaatt tttggagcaa
     3841 taaaggataa aaaatggcag gaaagaatat tatcatttct tgcctatatt ggaatttctc
     3901 tcccttcctt ctttatcgca atgcttgtta tattttttat tgcaaaaaca gcagttttac
     3961 ctcttggagg aacaacttct attttttatg aaaatgcttc aaacattgaa aaatttattg
     4021 attaccttaa acacttattt gttccgggaa tgattttaac aatttcaggt atagggtctc
     4081 tatatcgcct catgaaaaac aattttcttg aaacacttaa ctcgccttat atttttactg
     4141 cttatgcaaa aggaatcccg acttataaaa tatattttaa acatgctttg agaaatgcaa
     4201 taaatcccat gataataatt ttcggatatg aactttcagg aattttaagt ggtgctggac
     4261 ttgtagaaat tataactggc tggcctggta tgggaagact ggttcttgaa ggtgttttat
     4321 cttctgacct gtatcttgtt atggcttctc ttctaatagg agggattttg cttattttag
     4381 gcaatttatt tgctgatatt ttaattgctc tggttgaccc gagagtgagg ttaagatgaa
     4441 aagtatttta aagagattaa aaaataaaaa ttttttttca tactgttctt tctggataat
     4501 tattttcctt tattttttag caataatggc ggattttata tctccttacc cctataataa
     4561 acagttcaga gattatccat tatgtcctcc aacaaggata cacttttttg accataataa
     4621 aaattttcat ttaagaccat ttgtttatga aataaaactt gtgaatcctg ctttaaatca
     4681 atatgaaatt gttaaagaaa aaatatgtcc aatttatttc tttgttaaag gggagaaaag
     4741 aaaaatactt gggatattta attcagaaag atatctgttt ggaacaaaag agggtaaaat
     4801 ttttcttttt ggtacagata tgcacggaag agatattttt tcaagaattt tatatggagc
     4861 aagaatttca ctctcaattg gcataatagg tgtttccata tcttttttct ttggtttttt
     4921 aataggtgga atttctggat attttggagg aaaaattgat aatattttaa tgcgtcttgt
     4981 tgaaattttt atgatgtttc ctgcttttta tttgatgctt tctttaaggg ctgtttttcc
     5041 tttaaatctt tcttcttttc aaatatatct tttaatcgta ataattttga gtttgattgg
     5101 ctgggctgga cttgcaagaa ttataagggg tatggttctg tcaataaaag aagaagaata
     5161 tgttcttgca gcaagagcac ttggagtttc aacttttaaa attattttta aacatataat
     5221 acctaatact ttttcatatt caataactgc cattacatta tccattcctg gctatatact
     5281 tggagaatca actttaagtt tactcggact gggaattcaa gaaccatatc caagttgggg
     5341 aaatatgctt tctgtctgta ttggaaattt aagagtttta tcaagtgctc catggatgtt
     5401 aacacctgga tttttcatat ttctcacaat tatatgtttc aattttcttg gagatggatt
     5461 aagagatgtt cttgacccaa aaaactggta aaatggaaaa aattttaact gttaagaata
     5521 tctttgttgg taatgaggag attaaaattt taagaaatat aaattttgaa gttagaaaaa
     5581 aagagattgt ttcaattgtt ggagaaagtg gctgtggaaa gagtatgact ttgagagcaa
     5641 ttataaaact actacctaaa aatcttaaaa ttcttaatgg tgaaattttt tttgagaatt
     5701 tgaaaattac agaatacaat gaaatggaaa aaatcagagg taaaagaatt ggaatgattt
     5761 ttcaggaacc ttcttcatac ttaaacccac tcttcacaat aggaaatcaa atagaggaag
     5821 ggattaaaga aaatataggg aaaaaagaga aaaaagaaaa aatagtaaaa attttaaaag
     5881 aaattgggct ttctccagat gtttattaca gttatcccca tcagttaagt ggaggaatgc
     5941 aacagagggc aatgatagca atggctttaa taaataatcc agatttactt cttgcagatg
     6001 aaccaacaac agcacttgat gttacaactg cttctggaat aataaagtta cttaaagatc
     6061 ttgtttcaat atataacctt tcaataattt ttgtgaccca tgatatttcc cttgcaaaat
     6121 atatttcaga taaaattata gtgatgtatg caggaattat tgttgaaaaa ggaaactctg
     6181 aacaagttta taataatcct ttacatccat acactcaaaa actcataaat tgtctacctg
     6241 aaaaatactc aaagagagaa agaattaaaa caattgaagg agaagtccct tcttttaaaa
     6301 atttacctta tggttgtcct ttttctacaa gatgtgaata taaaaaaaga atatgtgagg
     6361 aaaaaattcc tggagaaatc gttattgaaa atagaattgt gaggtgtttt aaatatggaa
     6421 atattgtgga aagttgagaa tttaacaaaa gagtatgagg taaaaagtga atttaaaaaa
     6481 ataagaataa atgctttaaa aaatgttaat ctggaaattt ttaaaggaga ggttttcggt
     6541 cttgcgggtg aaagtggttc agggaaaaca acttttggaa aagtccttat aagattaatt
     6601 gaacccgatg atggaaaaat aatttacaaa gatattgaca ttacaagaat gccagaaaaa
     6661 attttaaaga aatatcgcaa aaattttcag attatttttc aggaccctta taaatcatta
     6721 aacccaagaa ttccctgtgg cttgactgtt atggaaggta taacaggaag taaaaaagaa
     6781 aaatttgaaa aaagtataga attattgaat cttgttggtc tgaaaaagga gagattttac
     6841 gattatccac atcagttcag tggaggagaa agacagagaa tagcaatagc gagagcatta
     6901 tcaacaaatc cagaatttat tgtttgcgat gaacccactt caaatcttga tgtttcaatt
     6961 cagggtcaga ttttaaatct ttttatagac cttaaagaaa aatttaatct tacatatctt
     7021 ttcataacac ataatcttaa aattttaaga tttatctctg atagaatagg tataatgtac
     7081 agaggagaaa tagtagaaat tggaaaaaca gaagaaatat ttacaaatcc aaaacatcct
     7141 tatacagaaa aacttataaa aagtgaaatt tagttattta tataatcaat cccttctatc
     7201 aaaactcaaa tctgttctat actggtaata aataaggcat gctgggtttt ctcccagtaa
     7261 tgaggtcttc taaaaaattg aaaaaaacct tttaaagcac caatactcat caatatccag
     7321 taaaatggag aaagtattgc aggtattaca agtttatacc attttctttt aagaacacca
     7381 agaatattaa ttaaaacaaa tattatattt ccgagaagta aaataggagt gacaatcata
     7441 aaaaatatgt tcgggaaaaa aatcctgtgt ggagaaaaaa gccagattaa tagaaaaacc
     7501 caagcaatcg gattaaaaca taaaacaaaa aaattaccac caactattag attgaagtgc
     7561 aaaaaatttt taaaaccaat ttttcttatt aatttaaaag gattccgtaa atgaacaaaa
     7621 tatgtctgaa tatatccttt aacccacctg cttctctgtt ttatccagtt tttaagttca
     7681 ctgtttgctt cttcccatgt tgttgtatca aggacttttg ttctgtatcc actcataaaa
     7741 attcttattc cgaggtcaca atcttcagtt acattaaaag ggtcccatcc ttttaattct
     7801 tttaatatat ctgctttaaa atgatttgaa gtaccgccaa gaggtatagg tgcttctata
     7861 ctgtctatac ctggcagata taggtcaaac caggaagaat actcagcagt aaaccatttt
     7921 gttaaaaggt tctgatttgg attataataa tttaattttg attgaagaca aactacatta
     7981 tcagaaactt ttctgaaacc tattaccgct ttttttaact ggtctttctc cggtatatct
     8041 tcagcatcat aaataacaag ataaactcct tttgcctttt ttaaaccaaa attcagtgct
     8101 tttccttttg ttttgggttt aaaatcagga acttcaatta ttctccagtg agaaggcaat
     8161 ttataatttt ttaaatattc ttttatttca aggtcatcct cttcaatcag aagtattata
     8221 tcaagtttat cttttggata atctatattt tccattgcca tatacagttg attaaaaatt
     8281 tccttttctt tatatattgg taatagaata gtataaagag gccattctct atttgaaata
     8341 atttcaattt catttatttt tatttcctta tttctaataa gccctctaaa gaccaaaaat
     8401 aatttgtgga tgcagatgat aaaataaatt gtagagaaga aataaaaaaa agtatatgtt
     8461 ttaaagaaaa aatgagaata aaggagataa aaagttccgc aaaaaaataa aaaaatgaaa
     8521 attttttgcc accatataag tttaatgtaa gcgttttctt caggcaaaag aaattcaaat
     8581 ttcagaaaat tattctccat aaaaattcac tttcaatgca atccattttt taaaaggagg
     8641 tatttcaact tttaaatttc ctttttcatc actcaaaatt tctttctcat caattttacc
     8701 tttttcaggg tcataaaatt ctactttgaa tttacctgtt cttttatctt ttaaaaagca
     8761 tatcatatta ttatattcag gaaaatctct tctttcaaca ttccttataa cactataatc
     8821 atacaggaaa acacttgaaa aaaatctgtt tccagttgct actgtaaaaa tttggttttt
     8881 ccccgctcca tcaatgataa cattttctga ctcaagggga aatttcaacc tgtcaacacc
     8941 ttttgtgtat tctgaaaaga ttttataaat gtaataaaga ttaaattcat ctatgaacct
     9001 gtgccaccac aaaaggggag ttcctgcaaa gggcaaatgg aaagaaaacc acaatcccat
     9061 tattatatct ctttttaaat tttcaaaact gcttcccata ggtgttccgc catactctga
     9121 aataaatgtc ggttttttaa agatagaatt aaagttgtat atacttctaa aaaaagttgg
     9181 aagatttgtt tgggaaaaat tataatatcc atttgttatg gtgtaatcaa taatatctga
     9241 cagaatttta ttatttacaa gaattgtgta atgtgcggta accatatgtt gaaaagggtc
     9301 tatttctttc aaatattctt tcatctcttt ataccattct gttatttcat ctctaagata
     9361 aaaattatga aacgcaccga taagatttat ttcatttata atttcccagg aaaaaatata
     9421 aggagaatac ccccatcttg caacaatata tcttaattta ttttttgtaa atttttttgc
     9481 cctttcatca gtgaaaaatt cattcggact ttttaaaaat ccaccatttt ttatattata
     9541 aggattatcc tgccattcct ggtcacacca tgtacttaac tgcccatgat ttataattac
     9601 aagttggaca taaatatcgt taagttctgc aaattcaata actttatcca ttttccacgc
     9661 atttcttaaa ttataataac taactcctct atatccgggc ctattttctt tccattcaag
     9721 tgcagcaaac catgaagcca tccagacctc tgtgaaatta attccatttt ctttcatctt
     9781 tttaaaaata ttctcataat aaaatgttcc actatcagaa tctactctct ttcccattaa
     9841 ctgtgcatat ctctcatctg ttggagaacg aatattaaaa ccaatcggat aaaaaaattc
     9901 accgctttta tatgaaaaaa atctattatc tatattgctt acctttacaa atttgttttt
     9961 tgtatcacag ggttcaacta taaaataatt aatatcagtt gttacttctt tatcatattc
    10021 tctaatctta acataaaact tgtatcttcc tggtttttca ggagtaaatc ttatcttcca
    10081 gtaaagatat ccaacaggag ttaaaacttc ttctccattt tctaaagacc tctcatattc
    10141 ctgataataa aacccaggaa caacaaaagt attattttca gggtctataa aaacaccatc
    10201 aatacttatt tcctctgggt caaaaggatt ggaatatttc tgcggtagaa aaaaagaaat
    10261 ttcaaatttt tcaaattgta agagtttttt tttacttata gaaatctcag ggaatacttt
    10321 aaaacttcct tttatctcat caatgtaaat tgaacctgta aatttagtat tcaaaaatat
    10381 ctttatacca atttttctta tattacatat tgttttctct gaccagggtt gaggatgtcc
    10441 tacattttcc cagaaagtac ttccaggttc aacattaata gttatcttat tccactgttc
    10501 cttttttaaa ataaaaagag gtgtttgata ccataaccat tcattatcct gtaagaaaaa
    10561 acatattttt atatcctcag gtgcactggc aggaatgtaa atattaaaaa ttatgttttt
    10621 atatattgaa aggtctttaa aaaactcttt ttcagcagaa ccttcaccag aatcatcaat
    10681 tttaaaaact aaagaccgtt tcccacttaa agatttctca tctgaaaaag ttatgtcctt
    10741 tatacagtta ttttttattg gagtaatttt ccagatttga atatcttcct caaaatcaaa
    10801 aaatatatct tcttttttaa cagagcctgt tgaagagtac tgagaaaata taaaacttga
    10861 taaaataaaa aaaattaata aaaatttttt cattacagtt ctatttcccc tccttttaaa
    10921 gcagattctt tttctttcaa aactatttca agagaattcc ttgcatcttc aggtgtaaca
    10981 acctgaaacg gtttattatg tattatatgg tcaacaaaat atctgagttc ataaaaataa
    11041 ccaccaaggt catctatatt tccttccact ccatttgctt ttattttttc aaaactcggc
    11101 ctttcaactt ttccagataa ttcataaaca gttacagtag gttttttatt agaactgaaa
    11161 tcaattgttg ccttttcaaa ttttgctgta aaactcattt caaactgaaa acttggagga
    11221 aaatcaaaac caccttctat ttcagcaatc aatccatttt taaatgtaaa tattgtaaaa
    11281 acatgtgcat caagtccatt ttttttagca attttagaat aaattttttg tggtttttca
    11341 ttgacaagat aaagtaaata atcggtatca tgaatatgta aatcaattaa agcaccacct
    11401 gatttctcct ggtctaaaag ccagtcctgc cagccccatg taggaagggg agaaactctt
    11461 ctcattgaaa ttgttaataa ttttccatat ttcccactct cataaatatc ttttaaatac
    11521 ttatattctg accaaaacct tataacctgt gcaacagcaa atctaacttt atttctctca
    11581 caagttttta tcatttcatc tgcatcttct actgttaaag caattggttt ttcacatatc
    11641 acatcttttc ctgcttctgc tgcttttata acatattctt tatgtaagaa tgttggtaga
    11701 catatatcaa ttaaagttat atcttctttt tttattagtt gttccgggtc aaaataaggt
    11761 attgttttaa attttgtggc aagtgatttt gctttatctc ctcttatgtc tgcaattcca
    11821 gcaattctta catcgggcag ttgtgaataa cagagagcat gcatatttcc cataaaacca
    11881 gcgcctatta tacctactct caccattttt tttcctcctt tttatctgct ttatttagac
    11941 aactttttta ctaattcagt ttagttttct tttggtatta attattattt taacattatc
    12001 atcgcaatag ttaaatatat acctgttcca agaatatcgg caagagttgt aattaatgga
    12061 gcactcgctg ttgcagggtc ttttttcaat tttgtaaaaa taaaaggtaa tgccattccc
    12121 attaaacatc ctgcaataac atttacgacc attgatacaa ccaccacttt acagatactc
    12181 aatcctcctc ttataatgcc cataaaagaa ataccaagtc ccattgttaa accaagcatt
    12241 aatgcaacca aaatttctct tccaaacatc tttaaccagt ccttcatcct aatttttccg
    12301 agtgccattg accttataat taaagtagct gactgacttc ctgcgtttcc tgctgtatca
    12361 acaagaaccg gtaaaaaagt aacaagaaca atatattttg ctatagtatc ctcaaatctt
    12421 ttaattattc cccctgtaaa caaatccata aaaagtaaaa aaataagcca tgtaaccctg
    12481 ctcttatata aatttttaat cggtgttttc aaaaggtctg ttatcaattc aacacctgct
    12541 gtttcaggag ttatagcaaa cattttcgtc atatcctctg tgtgctcctg ttcaactaca
    12601 tttagaacat catcaacagt aactattcca agcagaactc catcttcatc tataacaggt
    12661 aaaacaataa ggtcatactt tctcattaaa tttgatgctt cttcctgatc tttattcgct
    12721 tcaattgcaa taaaattaaa atccattatt ttttccactg tttcattcgg ctcagaaagt
    12781 atgagtttag ccagtgaaat atcatccagc aatttccagt tttcatcaac gacataaaca
    12841 acattgattg tttctgcctg tttaccaaat tctcttatat aagaaaatgc tttttcaatt
    12901 gtccagtcct tttttatagc aacataatct ggtgtcatca acctaccaac actgttttca
    12961 ggatatccaa gaagttgtaa tgcttctttt ctatctgcag gtgataataa attgagtaat
    13021 ctctgagtaa ccttcccggg caattcttca aataaatcag ttctttcatc aggaggcaat
    13081 tctgaaagaa tattctttat atgttcatct gttaattttg aaattaaatt ttcttttaca
    13141 atgggattta cttcatcaaa aacttctgca gataattttt tagggaggag acgaaatact
    13201 ataatcctgc attcttcatc aagttcttca ataagttcag caatatcagg agaaggccat
    13261 tcaacaagtg cttcttttaa attataccag tccttatttt ctatcagttc aattatttca
    13321 ttcttcagca atgtttttac aaatttttca tctgccattt atatttcctc ttcaaccttt
    13381 taattttagt ttttagattt ataaaagtca attttagttt ttaaggtata attaatatat
    13441 ggaaaaattt aatcttgtat caaattttaa gccatcagga gaccagccaa aagcaattga
    13501 aaaacttgtg aaaaatcttg aaaaaagaga aaaacatcaa attttacttg gagttacagg
    13561 aagtggaaaa acatttacaa tggcaaatgt tattgctgct gtcaataaac ctactcttgt
    13621 aatttctcac aataaaacac ttgcagccca gttatacagt gaattcaggg agttttttcc
    13681 tgaaaataaa gtgagatatt ttgtaagtta ttataattac taccagccag aagcatatat
    13741 acctgaaaca gatacatata ttgaaaaaga tgcatcaatt aacgaggata tcgaccggct
    13801 ccgtctggct gcaacaagtg caattttatc aagcagagat gtaattattg tagcaagtgt
    13861 ttcctgtatt tatccaattg gtagtcctga agaccataaa gcaatgatgt tacatttgtc
    13921 caaaaatcag ataataaata gaagatatat aatagaaagg cttattgaaa ttcagtatga
    13981 gagaaacgat tttgatttaa caaggggaaa attcagagta agaggaaata caattgaaat
    14041 aataccttct tatgaagaaa taggagttag aatatggatt attggagata aagttgtgaa
    14101 aattcagagg tttgaaccac tgacaggaaa tgtaatatca gaagaggaag aaatttatat
    14161 ttatccagca aaacattttg taactccaaa agataaactt gaaagagcaa ttgaatcaat
    14221 aaaaaaagaa cttgaagaga gggttgaata tttcaaaaaa aatggaaaac tccttgaggc
    14281 agaaagactt gaaacaagaa caaattatga ccttgaaatg ttatcagagg ttggttactg
    14341 tcatggaatt gaaaattata gtagacatct tgcaggaaga ttacctggtg aaagacccga
    14401 agttttgctt gattattttc cgaaagatta tttaattttt atagatgaat cacatgttac
    14461 agttcctcaa ataagaggaa tgtattgtgg agacagatca agaaaagaaa cacttgttga
    14521 atttggattt agattacctt cagcccttga taacagacct ttaaaatttg aagagtttga
    14581 aaaaatggta aatcaggtta tatatgtttc agcaacacct ggtaaatatg aattaaaaaa
    14641 atgcggggtt gaaagaattg gagaaccatc acctttaata gcagaacaaa taataagacc
    14701 aacaggactt gtagacccgc caataattgt aaaaccaaca gcaaatcaga taaatgattt
    14761 aataggaagg ataaaagaaa gagtcagtaa aaatcaaaga gttcttgtta taacaataag
    14821 taaacatctt gcagaagaaa ttgctgatta tttaaaggaa tttaatataa aggttcaata
    14881 tatacattat gaaatagaca cacttgaaag agttgaaatc ttaaagggtt taagagaagc
    14941 gaagtttgac gttcttgttg gaataaatct tttgagagaa ggacttgatt taccagaggt
    15001 ttcccttgtt gcaattcttg atgctgataa agagggtttt ttaagaagtg aaacagcact
    15061 gattcagata tcaggaaggg ctgcaagaag tttagatgga gaagtaataa tgtatgctga
    15121 caaaataact gatgcaatga aatctgcaat agatgagaca gaaagaagaa gaaaactaca
    15181 gatggaatac aacatgactc acaatataac tccaaaatca attgaaaagc caatatataa
    15241 aagtttagtt gaaattcttg gagggaaaag tagaataaaa gaaaaagaag aaaaattgaa
    15301 ggaagaagag tattatggaa aagatttagt aaaaattata aaacaacttg aaagaaagat
    15361 gatggcagcg gcaaaatcac ttgattttga aacagcaatt tattacaggg ataaaataaa
    15421 aaaacttaaa gaggaaaaat agatggacat aggaaatatg attaaaaaaa tggtagaaaa
    15481 ggttgaaagt tatagtgaaa aaataaaaga aaataaaaga aagcaggaaa gtgtctggaa
    15541 taacaaaaaa actgaaattc caattataac aggtaaaatt gacaatgaag ttaaaaatta
    15601 tcccgatttt aacatgaagg aatcatttta cgaccctgaa aaaatgttat atatgcaact
    15661 taaaggtata gttaatatcg tctcctctta ttcagatgca tgcccttctg taagatttaa
    15721 ttttggaaca ggttttattc cttcaatttt tggacttgaa tcagaaatat ttgaagataa
    15781 aatgccatgg ctgaaaaaac atctttcaaa agaagaaatt aaaagattaa aatttgatga
    15841 ctttgaaaat attgaagaaa tgggtcttat gaaaaaaatt ccagtttatc tgaaaactta
    15901 taaaaaatat ctctctaaaa atataaaaat ttatttgccc gatacacagg gaccatttga
    15961 tattgcacat cttataagag gggatgaaat ttttacagat atttatgatg attttgaatt
    16021 ctttaaatac atacttgaaa tttccacata tatttatata aaagcaactg aaaagttaaa
    16081 aaatttaatt ggagaaaata ttacagaaag ttatcacagt ggttcttatt atataaaaaa
    16141 tggaggtata agaatatgtg aggactcaac aacacttgtc tctccaaaac atctggaggt
    16201 tattttaact tacactcaaa aatgtttaaa atactttgga ggcggatgga ttcatttttg
    16261 tggaaaagca gaacacctat ttgatttagt gattgaaatt ccagaggtct cgagtataaa
    16321 ctttgggaat cctgaaaagt atgattttaa atatgttttt aaaaagttga atgaaaaaaa
    16381 taaagtttat cttggcagta ttccaagaga aaaagatgaa gaatggaaaa aatatcttaa
    16441 aagggtttat gaaatgtgtg aaggaaaaaa tttaatattt tcaccaacat taagagaaaa
    16501 tgaaaaaata gaagaaattt atgaatactg gggttcactg caatgagcgg gctttttaaa
    16561 atttcaaaac cagaccctct ggaatatgaa aaaagaggag attttaaagg acttataaag
    16621 ttgttgaatt ataaggatga cccttatata agatggaaat cagcagaaat tcttggaaaa
    16681 tataaagtca gagaagcagt cccttatctt ataaaaaaaa ttgatgaccc tgaatggcgt
    16741 gttagagcaa aaataataga ggcactcggg attataggtg atcaatcatc aattgaacca
    16801 ttactcaggc atataaaaga tgaatatcct gaagtcagat gttcagtatg ttatgcactt
    16861 ggcagattga aagataaaag agccactata aaattaattg aattaataaa agataaaaat
    16921 agatttgttc agaaagcagc aatagaagca attggagaaa taggtgatga aagtgcattt
    16981 gatgaattga taaaaatagc aagaaataaa ccacctgaga taaaagttct gattgcagaa
    17041 acacttggaa aaataggaaa aaatctggta aaaaggatga taaatttact tgaaaaaact
    17101 gaagataaag atataaaatg gataataata aaagcactcg gtgatatagg aaataaagaa
    17161 gcagggccta ttttgacgaa aaaccttaaa gatattagtt atgaaataag ggaagtatca
    17221 gctgagagtt taggaaaaat aaaatatgag gatgcagttg atattttaat tgaaatgttg
    17281 aatgataatg aatgggtggt gagaaaaaaa gttattgaag cacttgaaaa aataggaaat
    17341 aaaaaggcgc ttccatatct ttatgaactg gaaaaaatag aaaaagtaga agatgtcaga
    17401 gtaaaaataa gagaaacaat aaaaaccctt aatacacaat aagtcccaaa ggacacctac
    17461 gaggtgggaa aggagggaaa tatgaaaatt tatattgtaa cagatttgga aggagcaaca
    17521 ggtgtattta aattctcaca aacaagagaa agaggtcctg agttttatga agcaatgaaa
    17581 tttttaatgg gagatattgc agcggtgtgt gagggattaa aagaagcagg agtaaaagaa
    17641 atttatgtta ttgacggtca tgatggagga aacaatttta ttccggaatt gatgataccg
    17701 ggcgttcgtt atataactgg acgtccaaga ccaggggttt tttacggact tgatgaaacc
    17761 tttgatggag taattcttct tggttatcat gcaatgaatg gaactcctga cggtgtttta
    17821 aatcatacac agagttcaat tgccgaatta aaatattact atgatggaat tgagagagga
    17881 gaaatctatc aatcagcagt tattgccggc cattttaatg ttcctgttat acttgtaact
    17941 ggagatgaag cagcatgcag agaagcaaaa aaaacacttg gagaaaattt accaactgtt
    18001 gcagtgaaaa agggaataag tagagaggca gcaattttaa tacctccaga agaaacaaaa
    18061 attttactga aagagggtgc aaaaaaagca ataaagatgc tacctaaact taaaccatat
    18121 aagataaaat tacctgtaaa attaaaaata agaaaaattg ggcctgaagg cacaagtcct
    18181 gataacccat attttacaga aaaagaagtc attgttaaaa gtgctcttga tataataaca
    18241 ggcagtaaaa acccttgata cacaataagc cccaaaggac acctacgagg tgggagaggt
    18301 taggtaatta ttcttttttc agttattatt atgtctattt ttatatcatt ttcatcaact
    18361 ggtaatttct catcaaaaat ctgaaattca aaagcaagag atattttttt tgtttctgaa
    18421 ggaatatttt tcaaaaatct atcgtaaaat ccacctccca tcccaattct gtttttataa
    18481 atatcaaaca caacccctgg aacaattact atatctatat tttcaatttc tactggtctt
    18541 aaaaattttg gtcctgggat cccctttatt tcaataattt cttcagttga cttaatttca
    18601 gaaggtatta aaattttgtt tttccagtta atataaggaa gacaaataat ttttttatct
    18661 tcaattcctt tttttataat caggtctgtc cttacttccc tatcaaaatg ataatatgtg
    18721 aaaattacct ttgaattttt gtaaaaatca cttaatagaa acttttgctg tattttaaaa
    18781 ctcttttctt cccaatcctt aaattcaagt atctcccttt cctttcttat ctccttcctt
    18841 atttctgcct tcttttgttt ttgattcatt tttaagtttt tcaatatctt caagaaattt
    18901 ttttatctta ctttcatatc caatatccag tggaaaatag aattttttag ttccttctct
    18961 atatttttga ataacatagt gagaaggata atcatgagga tacttataac caatccctct
    19021 gccaagtttt tctgctcctt tatatccagt acctttaaga tggtcaggta cctcttctac
    19081 tttttcactt tcaacttctt tcattgcttc ttcaattgca agatatgaag aattacattt
    19141 tggagatgtt gaaagatata taactgtttg agcaaggata atttttgctt ctggcatacc
    19201 aacaacctct actgcttgat aacatgaatt tgcaaggata agtgcctgag ggtctgcatt
    19261 cccaatatct tcagaagcaa aaatcaacat tcttcttgct ataaatcttg ggtcttcacc
    19321 acttacaagc atttttgcaa gataatataa agcactgtca ggatcagacc ctctcatact
    19381 ttttataaaa gcagagattg tgtcataatg tgcatcatgt tttctatcat agataaaata
    19441 tttatcaaca aattctttaa ctttattcaa atcaattata atataaccat ctttctcaat
    19501 ttcagaagtt aaaatgcaaa attcaaaagt attcaatgct tttcttgcat ctccctgact
    19561 ttttttgcct ataaattcaa ttgcctcttt cgttattttt atcttataat tcccaagtcc
    19621 ttttttactt cttaatgccc ttttaattat ttcttctatt tcttcttcgc tcaaaggttt
    19681 aaattcaaat acaagtgatc ttgaaagtaa aggtgtattt atataaaaat aaggattgtg
    19741 gattgttgca cagattaaag taattgtccc ttcctctaca tctttaagta atgcatcctg
    19801 ttgaagttta ttaaaatgtg aaatctcatc aagaaataaa attgttcccc tcttttccaa
    19861 tctttgcctt tcccttgctt cttttaatac ttcccttaca tcagaaactg ttgctgtaac
    19921 cgcatttaaa gaaacaaatt tcttctttgt catatttgct attatcattg caagtgcagt
    19981 tttaccacaa ccaggtggcc cataaaaaac aagagaagga attctatcac tttcaattat
    20041 ttttctcaat ggttttcctt cgtcaagcaa atgtttttgt ccaacaaagt cctcaagttt
    20101 ttcaggccta actcttatgg aaagtggtaa attttcaaat tttttttcta cttcagattt
    20161 aaaaagtccg ttcattttag gttatttttg aaacaataat ttctccaata attgcaatgt
    20221 cttctatctc aaaaatttca gaagttgtct tgttaaataa aattcttaag tccgctggaa
    20281 attcgtcatc ttccggaatt aaatcaaaaa taaaaaacac atcagaaaat atattgaatt
    20341 taactgaaaa atcattaaat ctttctgctc ctatttctat actttttcct acaaattttt
    20401 ctggttcttt tccatatttt ttaattaaag gcaaataaat ccttgaataa atagatggaa
    20461 agtaaaaatt accttctggt aaacttttaa atgtaattaa ttcatcgctt tttttataag
    20521 ggaatttgca taaataacga aggattataa ctttttcttt atcattaata tctttgttaa
    20581 acttcataga taagatgtct atttttatta cttgattaag ataggtcaat tcaatttcgt
    20641 tttctccata aataaaatct tcttttaatt tctttttttt tattagtcct ttcagttcct
    20701 caaaatattt attaataagt tcagtatagg tttctgtcat tttaaagttt taaatatgag
    20761 tcagaataaa ttgattttcc aagaaggata ttaactgttt ttacataatt tcttttttct
    20821 tcatttaaac cagttaaatc aatctcttca tccattgctt tccatataag atttttaata
    20881 tcatctcttt ctcctctcac aagtgaaatc agttctttat cataggcatc aacaattgca
    20941 ctattcattc catatttcca aagcataaca agatatgttc tatttataag acctcttaac
    21001 tcattcaaag caccatttga aacatttgaa agaccacatg ttgaattaca atcagaaaac
    21061 aaatcattaa acatcttcat aaattcaata acacttacaa cttgttcctg ttgaaatctt
    21121 actggtaaaa ctattgggtc aaaatatacc ttatcatgag aaatttcata ttcatcaatt
    21181 ttagccaaaa aatttgctgc tattgctcct ctttcattct catcccttgg cattccttct
    21241 tttccaagta aaagtcctat aaatttacaa ttatattttt taacaagtgg taaaagttca
    21301 tccatccttt cttctgttgc ctgtattgag tttataattg ctttttctcc ttccatttca
    21361 agaccaactt tcaatgcctc aatatttgtt gtgtcaagtg ataatggtaa atcacatact
    21421 tctcttactg tttttataat ccattccatt aagaatggac cgtcttttct tgcaggacct
    21481 atatttaaat caagatagtc cattccatta tttttgcttt caattgctac cttctgaatt
    21541 atttcttttt ttctctcatt tattgcacct ttaatttttt tgttcataat gtttatattt
    21601 tcacctatca gaaacatttt tacctcctaa ttttaaattt caacattttt cagaaatgac
    21661 ggaatatgtg ctgcttctct tggaccaagt aaaatcttcc aatcaggtag ttcttcttca
    21721 agttctccat aaatctgtgc aacaacccca ggaataatta aaactttttt atttaatctt
    21781 tcaccaattt ttgtcttctt cacaagattt gctattgttt ctgcattaaa cttatcagca
    21841 gcccatgagg ttaaaactga aagaccttct gtatcctgaa ttaacaaata tgctggtatt
    21901 cttgaatttt ctatttctcc agaaacaata aagtaagtta gtgaaaaatt tgtagtaaca
    21961 aagacaggag aattttcatc tggttttcct atttcatata ttccttcagg agttgttaat
    22021 ggtctttgag ggtcagtgaa tatattcaat ctttcaatta gcagaggaaa taggacttcc
    22081 cctcttaatt ttgaaagtaa aactatactt ccatatttag caataaaaat agaagcataa
    22141 acaccttctt ttaaataatc atcggtaagg aaggatggaa ttgtcaaaat tggatatcca
    22201 aattctttaa ttttttttaa aatagcacct cttctgataa atataacatt cttaaaaagg
    22261 tctgatattt cattatttga aggaaatatt ataatttctt ctatgccttt atcctgtaag
    22321 gttttcacat tgcttgttat ttcttctatt gtctctcctc taacaatcac tggaagatta
    22381 aattctttcg caatatttaa aatctcctct gaaatttttt cagaaaaaat aatgggtctt
    22441 acatctttgc atatagaaag agaattttta attgtttctg aattatcact cataagtatt
    22501 aaattttttc ctgtttcata acatctttta acaatatcaa taaatttacc tttcccctca
    22561 tccttaattc caataaactc tgctttaagt tctattccta cccttgaata gttaaagtca
    22621 gtaatatttt ttatttctct gtttatctcc tgttcttcca tactttcctt tattaaaact
    22681 ccaattcctg ttggattata aaatgttttt tcatgcctgt ataaaactgt ctctcctcca
    22741 agattaactt tttctttatt acctacagtt attgttttta ttggaggagc acttactgca
    22801 gagagttgct tttttgcttc ttcacttata tgaggacatt gagataattc agctttacct
    22861 atcgcgattt gcattgcaaa agcaagacat gtcggaaacc cacattcttt acagtttgtc
    22921 tttggtaaaa acttaaaaat atcaagtggt tttaaagcca tttcccctcc tttttttaaa
    22981 ttataaatta gaaagtaaaa aattcaaaaa ttttttaact tcactgtaag cagatgaatt
    23041 ttcagaaaga tttaagagcc ctttattttc ttctattaaa ctataaattt cctcatcaaa
    23101 aggaattgtg aaagcaattg ggattaaagt tttaacctca tttaaatttt tcttcataaa
    23161 ttcatttaaa acaagatatg tttttttgat gcttaattca agagaattta tcattttgta
    23221 tatattttct gctgttctta aagagatttt atcacaaagg gaaacaataa aaagaatatc
    23281 aaggtctcct gatgtccttc ttgagatatg ctccattcct gcctcattat ccataacaat
    23341 aaagaggtag tttttttcaa ttttttccat ccactctctt aaaagattat ttgcataaca
    23401 ataacaacca ggtccttctg tctttcccat aacaagtaaa tcaaatccct tttcttcctt
    23461 aattgcctcc tgaatcctta tttcaagata ttttgatttt tccattccat aaggtaaatt
    23521 attctttatt ttttctatct catcaactat ttcaacaagg gattttggct gtggaatacc
    23581 aagattctca gcaagattac tatttgggtc tgcatctata gcaagtattg gttttttgtt
    23641 catttttaaa agagaaagaa taatcaatga agttaaagtt gttttacctg ttccaccttt
    23701 tcctgctaca ccaattttta atggtttcat ttttcaaaat cagtatgtgg tataaaaagt
    23761 gcttttaggt actcttccat ataaattgga cttcttgaca aatctatata tgtaagtttc
    23821 tttattatct cttttgacaa ctttctgaat ttctcactta aaataaaact ttctgctcct
    23881 aatagagaag tatttccaag aaatttaaat ctttcttcag gtaaattagg aaaaagccct
    23941 atctctattg atttttttat atttaaatgt ttcccaaatc cacctgcaat atagaatttt
    24001 tttacatcct caatttttat tccgagtgtt tttaaaacag tagaaattgc agaatatatt
    24061 gctccttttg ctcttatcaa gttatctata tctgcttgtg taattacaat cttttctcct
    24121 tttttatttt ctgcaactat aaattcttta atcccttcat tttcaataat tctctcattt
    24181 tcctttataa aattaccgtt tctgtcaatt attccatttt tataaagttc agaaagtaat
    24241 tcaatatatc cagaaccaca gattcctatt ggctctactc cacctattgt ttcaaaaaat
    24301 acatctccat tacttatttt tactttctgt attgctcctt ttattgccct taccccattt
    24361 ttaacaccac ttccttcaaa acatggacct gcagaagcag cacaacatac taaaaaatct
    24421 ttattcccta caacaatttc accatttgtt ccaatatcaa ggaaaataga aagtacatct
    24481 tctttataaa tacctgaaac tataatacca gaagttatat ctccacccac ataaggaaat
    24541 cccccaggaa ctatttcaca tattccagaa gggtttattt taatacctat ttctgcactt
    24601 ttaattaaag ggaattcatt tgcaataggt atatatggtt cttttcttaa aaatgaagga
    24661 tttattttta aaagaaggtg aaccatggtc atatttccag aaattgaaat tccagttatg
    24721 tcatttattg aaataccaca cttttttgat aagtttatta tcatatcatt tattgtttct
    24781 gtcacaacat gatgtaaatt ttcaagtcca tctttttttt cagcatatac aatccttgaa
    24841 attatatcat ctccaaaaga tgcttgtcta ttaaatgtta tttctgtttt tagaacatca
    24901 cctttattaa tatcaattaa acttgctgaa atagttgtag tcccaatatc cacagcaatc
    24961 ccgtaatttc ttttagatgt atcacccggt tctatattaa gtatttccac catgtcatca
    25021 ttttctatta aagtcactgt tatctcccag aaactgcttc ttataagatt tggtaatttt
    25081 cgtaaatttg aaatatcaat tctaaatttt tctttattta tatattttct taaatttctt
    25141 ttaactcttt cataatcact taaagtatcg gaaaaatttg gggatggtaa tctcaaaaat
    25201 atttttttaa taagcggaga aaatgaaaat aattctttta catcaattct aatatgttca
    25261 aattcttctc ctttttcaaa ttctctctct attttttcag gaataactgt ctcaggaagt
    25321 aattcaattt ccatattatc aagtattgtt gttctacatg caagataaat attatttctt
    25381 ttctcttctt cagtaatttt ccctgttggt tcagaataaa aatcaccttt taatattttc
    25441 actttacatc ttccacaaat accttttcca ccacaacttg atgtaataaa aatatttcct
    25501 tttattaaag cagacaaaat atctgttcct ttccttattg gtatcttttt atcaagtggt
    25561 ttaacagtaa ggataaaata ctctttcatt ttaaaatttc ttcaatatca acattttcct
    25621 ctataatttt ttttgctatt tcaatcttat ctaaatcgtt ttctgttaat ttaacaacaa
    25681 gttcattaat ttttgaagca actatatatg tttcttcttc tgctacaatt gttggaaaat
    25741 catatttttt aataagttca agtattgttt tatgaggata tataccacct gttaaaacga
    25801 taccttttat taaattctgc attgatgtag aaagtgcagc aagaattatg tcttctctgt
    25861 cgcctggggt tatcaataat gaatctggtt taaaataatc aagtgcatgt cttggagtca
    25921 ttgcaccaac aattacattc tttatttttt ccctcatttt attccaacca gtaattattt
    25981 ttccattaac tgcatcacat atttgaaaaa gatttggttg agtaagataa ggaacataag
    26041 gaataaggcc taaaagtttt aaatcaattt tttggaaaaa cttatttagg tatttttcaa
    26101 gtatatcctt tttttcctct tgaactttat ttataatcac tcctttaata gaaacatttt
    26161 tttctttaaa aagagaaaga ttaagagata tttcatctgc tggttttcct attccaccaa
    26221 ttgaaattaa aataacgggt gaatttaaaa ttcttgctac ttctgcattg tttaaatcaa
    26281 aaattgagcc aactccagca tgtccagttc cttcaattat aaccatttct ttatcctcag
    26341 aaatcttctc ataactcttt ttaatctttt caacaagttc atctttattt ggattatcaa
    26401 gataatttct tgtaaaacct tcctcaacag caattggatt catatcttca agttcattct
    26461 ctaatttaca aattcttttt agtaaaaccg catccttatc tactttttta ccatcaataa
    26521 ctaaatattt ttgaccaact ggtttaataa atccaatttt atcaacatat tttttcaaaa
    26581 caaggaaaag ccctatactt ataactgtct ttctatcatt ttgtcttgtt gctgcaataa
    26641 atatattttt tggcataatt taattatacc aaaaatttga cttttttgaa aatctgctct
    26701 aaaattattt tcttatgaaa atagcaattt ctggtaaagg tggaacgggt aaaacaacaa
    26761 tatctgcttg tatttcaaaa gtatatcttg aaaaaggaaa aagagttata ttgattgatg
    26821 cagaccctga cagcaattta aaatcaactc tcaacataaa aaaagaaatc atacctttaa
    26881 ttgaattaaa agagattatc gctgaaagga cagggacaaa accaggagaa agagcaatta
    26941 tatttaaaat gaatccagaa gttgatgata tcccagaaag atttttttac agaaatggaa
    27001 atttacttgt aggaattatg ggaactgtaa ggggtggtgg tcttggatgt atgtgtcctg
    27061 aaaatgcatt tttaaaagca gttataagga atttaatttt aaaaagagat gatgttgttg
    27121 tacttgatat ggaggcagga attgagcatc ttggaagagg aacaactgaa gggattgatt
    27181 ggttattaat tataactgaa ccaaccgaaa aaagcattga aacgactgaa agaattgaaa
    27241 aacttgcaaa agaaataaag ataaaaaaaa ctggtgtaat tggaaataaa attgaatctg
    27301 aaattgagaa aaattttata attgaaaaac taaaaaattc agaaatactt ggctatatcc
    27361 cctatttccc tgaaatgaaa aaaggagaaa tagaaggaaa gacgccatgg gaaagtgcac
    27421 ctataatttt gaattatatt aaagagatag ttgcaaaaat ggaggcaaaa aatggaagat
    27481 aaaaaagttc ttggagaaac atatgaagaa ggaaaaccaa aaaaatctct taaatggaaa
    27541 aaatttgaaa ataaagatga gatgataaaa tttataaaag taagtgaaag atattggttt
    27601 ggtgaaggat atggaagtga aaagagaaaa acacctgcat aaaaaggaga aaatatggac
    27661 gaattaaaat tgaattatac gggaaaaata agagaaattg aaattggtat aggcgaaaaa
    27721 aagataaaag ttggtggaca gaatggattt aattttcatt attttgaatg tgaattccca
    27781 aatgaaccta taatatcttt tgaagtatgg gatagtcaac ctgaagaatg gcctgaaaat
    27841 tgtttatctc cttataaaga tgtcataaat gaccctttaa gttgggcaaa aaaatgtgta
    27901 aatgaatatg gtgctaaatg tttaactata tttttaaatt caacagaccc gaatggaatg
    27961 aataaaagtg cttcagatgt aagaccaata gttgaaaaaa ttgtaaacgg gatagatgtc
    28021 cctttaatct tctggggaag tggaaatgtt gaaaaagata tagaagtttt aaaaatggtt
    28081 tgtgaagtgg caagtggaaa aaatatagta gttggaccag ttgttgataa gaattataaa
    28141 cagataggtg cttctgctat tgcttataaa caagtggtaa ttgcttcaac accgattgat
    28201 ataaatcttg caaaacaatt aaatatttta cttttagaac tcggtgtacc tgaaaataaa
    28261 ataattatgg acccaacagt tggttctctt ggttatggaa ttgaatatac atattcagtt
    28321 atggagagag atagaattgc tggactttta cagggagatg ataaacttgc ctttcctatt
    28381 tattgttatt tatcaaaaga ggtctggaaa attaaagagg taagtattcc tgaaaacatg
    28441 gatattaaac ttggcaaaga aaaggaaaga ggaattatgt ttgaggcaat aacagcaact
    28501 ttacttttac ttgcaggagc agatattata gttataagac atcctgaaac agttaaattg
    28561 ataagtgaat tgatggaaga aatgaaatca taaataggag gaaaaatggc agaattaatt
    28621 gaaacaaagt tctcaacagt tgaagagaga aaaaaaagac aacagcctca ttgtaaattt
    28681 ggacttcttg aaatttgttg cagattatgt tcaatgggac cttgtagaat aacagaaaaa
    28741 agtccaaaag gtgtttgtgg agcaaatgaa gatactattg ttgcaagaaa ttttttgagg
    28801 aaaatagcag ttggagcaag tgcacattca gaccatggaa gagcagttgc tgaaacactt
    28861 atgaaggttg caaggaatga agcacctgga tatgaaatta aggatgagtt taaacttatt
    28921 aaacttgctc ttgaatttga tatagatgtt aaaaataaaa aaacagaaga aattgcactt
    28981 gaagttggag aaacatgttt atctcaattt ggacaacagg aaggagaatt gatttttaca
    29041 aagagagcgc cattgaaaag acaaaaaatt tggaaagaac tcggtataac accaagagga
    29101 attgacagag aggtcgttga atcacttcat agaactcata tgggaactga ccaagattat
    29161 ctaaatttga tgaaacaagc acaaagatgt gctttatctg atggatgggg tggctcaatg
    29221 attgcaacag accttcaaga tgttttattt gggactcctg aacctattat tggaaaagta
    29281 aattttggtg ttttgaaaga ggattatgta aatataattg ttcatggaca cgagccactt
    29341 ctatcagaaa tgattgttat tgctgcagaa gaccctgaac ttttaattca ggcaaaatca
    29401 aaaggagcaa aagggataaa tataggtgga atatgctgta ctgcaaatga aattttaatg
    29461 agacatggga tatcaataat tggaaacttt ttaaatcagg aaatggcaat tacaacagga
    29521 gcaattgaag ttatggtagt tgatgttcag tgtataatgg cttcattacc tgaaattgcc
    29581 tcatgttttc atacaaaaat tataacaaca tctgaaaaag caaaaatacc gggagcaatt
    29641 catatccagt ttacagaaga aaatgcactc caaaaggcaa aggaaataat taaaatggct
    29701 attgaaaatt ttgaaaacag aaaaagagaa aatgttgtta ttccggaaag ccagatggat
    29761 ttagttgctg gtttttctta tgaaagtatt tattatattc ttggtggaag atttagagca
    29821 acttatagac ccttaaatga taatataatt aatggaaaaa taaggggaat tgctggtgtt
    29881 gttggttgta ataatccaaa agttgctcat gatgagatac atataaaact tgtaagtaaa
    29941 ttgatagaga atgatattct tgttcttcag acaggttgtt cagcaattgt atgtgcaaaa
    30001 gcaggattcc ttgattctgt caaaggatta aattatgctg gaaaaggatt aagagaggta
    30061 tgtgaggcag ttggtattcc acctgtaatt catatgggaa gttgtgttga taattcaaga
    30121 attttgatgg ctgcaacatg tgttgttaaa gaaggcggac ttggagatga tatatctgaa
    30181 ttaccagcag caggagcagc acctgaatgg atgagtgaaa aggcactttc tattggtcag
    30241 tattttgttt cttcaggtgt atttgtagtt tttggtgtta cctggcctac atttggaagt
    30301 aaaaatttaa caaaatattt atttgaagat attgaaaagg aagttggtgg aaagtgggct
    30361 tttgaatcag accctgataa aatggcagaa cttatgatta aacatataga tgaaaaaagg
    30421 aaaaaacttg gaatagataa ggcaagagaa agagtactct atgatatggc tatgaggaga
    30481 gaacttgaat gataagaatg gttgataaaa ataaaaaaat aaaactttca aaactaaaag
    30541 atatggcaga aaaaatgttt gataattttg ttaaatgtgt tgtggatgtg gaaagtgaaa
    30601 ttatggttgt tggtggagaa ttacatacag acgaagaagc acttttactt gaaactggtt
    30661 ctaaacagga aaatctatgg ggtataaata tatatcctga aaaaaaagat gatgaattta
    30721 ttgaatttga ctcaataata aatataagac cttcttttgg aaacaaatca agagaagtgg
    30781 aaaatccaga
//
LOCUS       DAOSAT010000011        30025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_1137_length_30025_cov_5.797965, whole genome shotgun sequence.
ACCESSION   DAOSAT010000011 DAOSAT010000000
VERSION     DAOSAT010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1137_length_30025_cov_5.797965"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..703
                     /locus_tag="PLF90_01750"
     CDS             122..703
                     /locus_tag="PLF90_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOK56042.1"
                     /translation="MKNQKKLEFLEKEVKNHIELVKIVFSPSFNENLIKISETIVDCI
                     KNGNKILLCGNGGSSADCQHFCGEMVNRFKKEREPLPFISLTTDTSVITSIGNDYSFE
                     DIFSKQVKAIGKEKDILICFSTSGESKNVIKAAKVAKEKKMKVISLTGKTPNTLEKIS
                     DFIISIQSKETEKVQQIHLIIYHLLCYLIEEEF"
     gene            706..1188
                     /gene="rfaE2"
                     /locus_tag="PLF90_01755"
     CDS             706..1188
                     /gene="rfaE2"
                     /locus_tag="PLF90_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOK56043.1"
                     /translation="MCKIKTLKEIKKISERLKKEGKTIVFTNGCFDIIHPGHIKILKK
                     AKSMGDVLIVGLNSDKSIKKIKGEKRPIIDQRGRCEIMSSFWMVDYIVLFEEKTPEKL
                     IKSILPNFIVKGSDYREEEVIGRDIIEKYGGKVVIVPLNKKYSTTNLIRKINENFKDY
                     "
     gene            1166..2209
                     /gene="thiE"
                     /locus_tag="PLF90_01760"
     CDS             1166..2209
                     /gene="thiE"
                     /locus_tag="PLF90_01760"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOK56044.1"
                     /translation="MKILRIIDVNFNRVREGIRVIEESVRFICEDNEILKKIKEIRHK
                     FSKGLLYYFPSDKLKSYRFVETDIGKTSDIKQKLNLKGLIETNFFRVEESLRVLEEYS
                     KVLNPDSFSFFHDLRFEVYRLEKKVITRISRKKIKIPSLYVILNLKENEKEFLKFAEK
                     VIKGKPDIIQLRYKGENSKFFLKIAKTLKKIIPDEIIYLINDRVDICFLCEGDGVHLG
                     KNDLDVESVRKILPDKIVGVSTGNIEDVKEVKNGDIDYIAIGSIFKSPTKPEKKPVGK
                     DILRRIKEIISIPLIGIGGINIENAGEVIEKGADGVAVISVVENSENPEKIIKKLKEE
                     IKKGWKKKGKRII"
     gene            2179..4041
                     /gene="ftsH"
                     /locus_tag="PLF90_01765"
     CDS             2179..4041
                     /gene="ftsH"
                     /locus_tag="PLF90_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOK56045.1"
                     /translation="MEEKGKKNNLNFLKGFLFWVLVIIFIFALLKFSEVEGKKINLIY
                     SDFINEVKKGNIEGVLSIKKNIVYGKLKDGRNFYTFIGEDPDLNRILIDNNISFKYEP
                     GNWIAELIPYIIPALIFFFIFWMFIKQANIERNRVMSFARSTPIIPNPRNRVTFEDVA
                     GCEEAKEELKEIIEFLKNPKKFQKLGGKIPKGVLLIGPPGTGKTLLAKAVAGEAGVPF
                     LSMSGSDFVEMFVGVGAARVRDLFRQAKRLAPCIVFIDEIDAVGRQRFAGLGGGHDER
                     EQTLNQLLVEMDGFQTDEGIIVMAATNRPDVLDPALTRPGRFDRTVVVTLPDVKAREK
                     ILKVHTKNKPLDSSVDLSLIARATPGLSGADLANIVNEAALLAAKKGKEKIDMSDFEE
                     AKDKVLMGVERKSLYVSEEEKKIIAYHEAGHTLVQKSLPEVYPVHKVTIIPRGQALGI
                     THILPDKDRFLESDTYYKNSLAALLAGRATEEIVFGRKFTGSENDLRVATEIAKKMVC
                     EWGMSENLGPISFRPHEAVFLGRDLVQQREFSEETSREIDKEIKKILEEAEARAKEII
                     MKNRDKLDILVKELLEKETLTSEDIDKILGIEKGEENGHSENRKGDGTDNRSTG"
     gene            3986..4555
                     /gene="folE"
                     /locus_tag="PLF90_01770"
     CDS             3986..4555
                     /gene="folE"
                     /locus_tag="PLF90_01770"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018393794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOK56046.1"
                     /translation="MDIVKIEKGMELIIEALGKNIDKKKLKDTPKRVAKMYEEIFSGI
                     DVDPKKVLGKVFKEDFNELVLVKDINFYSICEHHFLPFFGKAHVAYLPDGNNVVGISK
                     IARLVDVLSKRPQLQERMTNQIVDIIMDVLTPQGAMAIIEAEHMCMIMRGIKKPGTKI
                     VTSAMRGIFLRDLRTRTEALNLISPSIRF"
     gene            complement(4556..4729)
                     /locus_tag="PLF90_01775"
     CDS             complement(4556..4729)
                     /locus_tag="PLF90_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56047.1"
                     /translation="MKGIFIILGFLLLLGGIFLTFKPRLTYEKESTSDIIAVAGIILM
                     IAGIVILLSPFMK"
     gene            complement(4736..5926)
                     /locus_tag="PLF90_01780"
     CDS             complement(4736..5926)
                     /locus_tag="PLF90_01780"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOK56048.1"
                     /translation="MAKVITFGCKVNQYESQLIKENIEKDDNFKSENIVILNSCCVTE
                     KVEKEVKKKIKKLLKEGKKIYLTGCLIKKNGKLRELENINFVERDFFLKFKDKIERFD
                     EHTRAFVKIEDGCENFCTYCIVPFVRGKVKSRKEKDIIEEIKKLGENGYKEIVLTGID
                     LGSYGKDTGEKLLSLLEKIEEIKEIKRIRLSSIEIYYINEFFINSLKKINKFCPHFHI
                     PLQSGSDRILNLMGRRYTFNDYLEKIDMIKENIDRVTFTTDVIVGFPGEKEEDFEKTC
                     MAIDKIKFLKVHIFPYSERKNTVAINLPDKVEEKTKKERFKKILKISNYSSKKVKENF
                     IGKKLEVLIEEKTGNFLKGYSENYIPVIVNSDKNLKNEIISVTAEKILKNFLSGKIIK
                     EKGE"
     gene            complement(5928..8177)
                     /locus_tag="PLF90_01785"
     CDS             complement(5928..8177)
                     /locus_tag="PLF90_01785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK56049.1"
                     /translation="MEIVTKTRFFKPTEYSIYKPKICFLSTYIPKPCGIATFTNDLLR
                     HIDNYNILIPSKVIALNGKDDTYNYPEEVVRIITIEEIEDYRKAGEFVNESEIEIVGI
                     QHEFGIFGGEYGEYLLEFLEHLKKPCVTTFHTVLPNPPQKMREIVRKISEKSSYIVVM
                     TNIAKELLNDFYGIAKRKIELIYHGVPFSYLRPTNYFKEKLNLNNKIVLSTFGLLSRG
                     KGLEYVIYSLPSILKEFPDVVYLIIGATHPNIVKQEGESYREFLIKECERLNIVNNVK
                     FVNKFLTLEELLDYLGATDIYITPYLNPGQITSGTLSYAIGCGKACISTPYLYAKDIF
                     ENGKRGLIVNFRDPESISDTVLYLLKNPEKKKQIEREAYKLGQKMTWEKVAWEYLTLF
                     SRVSSSRGESASIYFEDRLLPIKLTHLETLTDDVGIIQHAKFSMADRKTGYTIDDNAR
                     GLVVVTKHYNMCIDQKSLNLINIYLSFIYYMQKSNGRFHNLLGYDRNFLDTDGGDDCF
                     GRCIWAIGFLLSSDFIYENIKGAAKHIFDLALPQIENINSLRGMANCLAGLYYYLKTE
                     NNERVREIAKKLAERIIENYQNSSEPNWKWFENIITYENAKLPCGLLYAYEILEDKKY
                     FDIGMETLNFLIDLTIIENRFIPIGNNGWYIKGGKRAYYDQQPIEAACMIEALKKAEK
                     LTGDEKYGNLSLIVFDWFLGRNTKGEMMYDPVTGGCYDGLSKSGPNRNQGAESTISYL
                     LARLEIEEI"
     gene            complement(8180..9238)
                     /locus_tag="PLF90_01790"
     CDS             complement(8180..9238)
                     /locus_tag="PLF90_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOK56050.1"
                     /translation="MKALLLLGGFGTRLRPFTITTPKALLPIVNYPFISYQFELLKNY
                     GIVDVVLGVGYKGDDFKKAIEIGKQMGIKVYLSYEKKPLGTGGGIKNAEKFLKGKDPF
                     FVFNGDVLADFNLEKILNFHKEKNAFITIGMIKVPDPSSYGLILTDDDMKIIKFIEKP
                     KKEEIITDTVNAGVYIFQPEVLEEIPENKEVSVEKETFPQILQKGKDMYGYIHYGYWL
                     DVGTIDKYKKGNFDLIDGKIELLYKKSEKNIEKKDNVLIGNNVIVEGKLIADENVIIG
                     ENSIFKGKVIIGKKSFIGKNCIIENSIIFGNVFIGDECIIENSIIGDSVIIRNNCLVK
                     NSAIGDNSYINQFSKISE"
     gene            complement(9366..9929)
                     /locus_tag="PLF90_01795"
     CDS             complement(9366..9929)
                     /locus_tag="PLF90_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK56051.1"
                     /translation="MEKKFYTTGELGKIIGVSRITVYKWIIDKKIEGVRLPGGRWVIL
                     KKEVERIMKERKLLGYETEKEVKILIGDDNVDISNSLKEFLERRNTNFRIITAYNGFE
                     VGKYLYSFKPEILILDIFMPGINGFEVLNIIKNDPNLKNIVIIVITGHPEEENIKKAK
                     EEGADYIFIKPFDYKEIEKTIKNIVQI"
     gene            complement(9931..11067)
                     /locus_tag="PLF90_01800"
     CDS             complement(9931..11067)
                     /locus_tag="PLF90_01800"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK56052.1"
                     /translation="MQEKLNFLGNLGIFEVSEYINQKERFTSFIYPSVCGNRIVKLFK
                     VLLTNNCKFNCLYCANRRDRDCPRYSFNVRELADIFYSMWRKGIVNGLFLSSAINGDN
                     NETQEKMLEVVEILRKKYKYNDYIHLKILPGVDEYLIKKGKIYADRLSLNMETVNSEY
                     MKRIAKEKDFSKNLLLTLKKLSDLNKESPLKNGITTQIIIGSSEEKDREILSFSFYLY
                     KNYNLSRVYYSGFIPVLKTPLENKKPCSLKRQVRLYQADILIRKYKFLPSEIPFDRNG
                     NLYLNRDPKILWAERNKDFFPVEINKADLYQLIRIPGVGIKTAKKILEARKEGKIKSE
                     ITLKKIGVRLRKALPYILINGKRIKKQQEQKEKDFYYTIFDEVV"
     gene            11116..12246
                     /locus_tag="PLF90_01805"
     CDS             11116..12246
                     /locus_tag="PLF90_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK56053.1"
                     /translation="MNERERYKKTLLFEKVDKIPFSPGSPRESTLKRWHKEGLPENVN
                     WFEYICEKIGIKIEKPKKTKINLSYSFFLNPLFEEKVIEKKNGHLIVQTEKGEIVEID
                     EKYDFTYLRSAKDFVTRKWHKFPVENLSDWEDMKKRYNPESPERIDPEIDKKAEILKE
                     RDWVLGISFHGPFWQLREWCGFENLCIFMAEKEDFVLEMANFWKNFVSEILKKLILKV
                     QFDYAVINEDMAYKEKSMISPSMIKKYLMPCWVEWTSLLKKSGCEVVMVDSDGYIEEI
                     IPIWIESGINCTIPVEIAAGNDPVKFRKKFGKNMAYVGGIDKRKIAKGGDVLKKEMDR
                     IIPFLFKDGGYIPSCDHGVPPDISFENFIEYCKYLAKYTGYL"
     gene            complement(12243..13001)
                     /locus_tag="PLF90_01810"
     CDS             complement(12243..13001)
                     /locus_tag="PLF90_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK56054.1"
                     /translation="MNSIKFLGTAGARFVMISQLRYSGGIWFSLEGIQFLVDPGPGTL
                     LRAVKSKPKLNPVNLEGIFLSHRHLDHSCEINLMIEAMTDGGFKKRGVVFAPYDCLYH
                     DPVILLYCRDFVERIEILEEKKDYKLKGLNFEIPVKLRHGVDTYGFKINLKNTVISYL
                     PDTSFFPELIDYYKNSEILIINTVLYEKKENIQHLCLSEAEEIIKNIKPKKAILTHFG
                     MTMLKNKIWEKKDQISERVGTEVIIANDGMNFEF"
     gene            complement(12998..14197)
                     /locus_tag="PLF90_01815"
     CDS             complement(12998..14197)
                     /locus_tag="PLF90_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK56055.1"
                     /translation="MGRKKKSVKNKEATKIEDKINEIVELGKRKKYLSWDDINEILPM
                     DMTDEEDINELFDELEYYNIDILEDEEEEDVFFVEKEKKIDIDDARNPLRSYLKGAGS
                     YNLLTHEEEIEIAKGIEEAKKQLNKLSKKSNPSKKQIEKYEKQLNKLREKLINSNLRL
                     VINIAKRYSNPKLSILDLIQEGNIGLMKAVEKFKYKTGFKFSTYATWWIRQAITRAIA
                     DHSSTIRIPVHMIEKINKVKKIEAKYSQLGEENELSEEEIAREMNLPVEKIKNIKKSM
                     RPDPISIDSPIGDEERATIGDFIEDKFNPNPLSYTRHSLLKEEIEKALSILDEREELI
                     IRLRFGLDGEGYPRTLEEVGQIFNLTRERIRQIEAKAIQKLRNSYKGKKLQIFTHGIF
                     GSKSFIK"
     gene            complement(14201..15943)
                     /gene="dnaG"
                     /locus_tag="PLF90_01820"
     CDS             complement(14201..15943)
                     /gene="dnaG"
                     /locus_tag="PLF90_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOK56056.1"
                     /translation="MEGGTLGSEVEEILEKIDIVEIVSEYINLKKAGKNFKALCPFHP
                     EKTPSFFVSPEKQVFHCFGCGIGGNALKFLMNIEKISFYDALNILAKKAGIEISKKEN
                     IEESIEKKKILKANEYAANLYNKTLFSTYGKIALEYLYQRGLTDEQINKFSIGFAPSN
                     NYLIKKLEEEKLNKKDFLLAALLDEEGKEDTFKNRIIFPIYNIKNEIIGFGGRTIDDE
                     IMPKYLNIRENIVFNKSNCLYGINWGKENIKSKGFVIFVEGYFDVLKMHIKGMQNTVA
                     PMGTTITELHLNLMKKLTDKILLLFDGDDPGIRAVLRNLETIIKKGFEIKICMLPSGF
                     DPDKFIDEYGIKSLKNFIENSQDFVDFSVSINSQIYDIKNPKEKSAIIKETLKFISKV
                     PDEIERYEFLKKLSEKMEIKIEILEGYLDAMIEKDIETEYITLSKINQENQNSAEKFL
                     IEIVINDKKYFEKLFEFKGQLTEKIEKIITAGEKLINKKLEINPSLLMGECNDEELAS
                     FISSIAIKDNNKISEELKEKIFNDCLKKIKKKKLLIEIESYKKKMNEKLEKGIDYKDD
                     LKEMQKLLYKLKKE"
     gene            16028..16516
                     /gene="lepB"
                     /locus_tag="PLF90_01825"
     CDS             16028..16516
                     /gene="lepB"
                     /locus_tag="PLF90_01825"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOK56057.1"
                     /translation="MFKLIRYFILIGIVFFTGYIFGIKDVKFLKIISSSMEPTLSIGD
                     KIISVKSNDIKRKDIVVLLSPEGEKEFLIKRVIGLPGEKIKIEKGYVYINGEKLQEPY
                     IKERPEYLFKEIEIPPGTYFLLGDNRNESEDSTTWGPVEKNFIVGKVLCRYHPFRKFK
                     IF"
     gene            complement(16568..16837)
                     /locus_tag="PLF90_01830"
     CDS             complement(16568..16837)
                     /locus_tag="PLF90_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003338869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOK56058.1"
                     /translation="MNKGDLIEAVAKVVCTKKEAAAAVDAVLDAIKGALKKGDVVTLV
                     GFGTFKVVERKARKGRNPRTGEEIKIPKKKVPVFRAGSELKKAVK"
     gene            complement(16960..17586)
                     /locus_tag="PLF90_01835"
     CDS             complement(16960..17586)
                     /locus_tag="PLF90_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOK56059.1"
                     /translation="MNLWLLAVKGGYIMIPIFICGVLTLAIIIERFIYLKNSQIDSEK
                     FIKDIEDLLKRRKIREAIEKCENQNKPVPNIIKAGLLKLDRTRDEIKEAIDDTANHQI
                     PELEKYLVILATIAVISPLLGLLGTVTGMIKAFMVIEMKEGLVNPGELARGIWEALVT
                     TVGGLVVAIPAYLSYNYFVSRVNNLILEMEKSATRLIDLIFLLKSEEE"
     gene            17716..18054
                     /locus_tag="PLF90_01840"
     CDS             17716..18054
                     /locus_tag="PLF90_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56060.1"
                     /translation="MKKNVLISAISVIIVLVFISGCGKKKEEELTPLNAPLKYGEVMS
                     KTMKKAKAMDGVIYLKNKINTFQIQEGRYPYSLDELVEKGYIKKEEMPKPPEGMKFVY
                     NPKTGEITVK"
     gene            18067..18600
                     /locus_tag="PLF90_01845"
     CDS             18067..18600
                     /locus_tag="PLF90_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOK56061.1"
                     /translation="MEFYEVIKTRRSVRNYKKDSIPDDVLKRVLNAARIAPSGNNRQP
                     WKFIIIKDRKIKEKMIALCDGQEFVAESPVLIVACGKNIHYNRGRWMGDYSVIVDVAI
                     AVDHLTLAARAEGLGTCWIGSFDNEGIKKFLGIPEDVNVVALTPLGYPENPNVFKETK
                     NRKSLEEIICYDKWEEK"
     gene            18585..19496
                     /locus_tag="PLF90_01850"
     CDS             18585..19496
                     /locus_tag="PLF90_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56062.1"
                     /translation="MGGKIERKKFKKIKYFIIPFLIILFFIFSFIVFKINLIKNINLT
                     EKWKIKDINFSSGEINLKDLTYKETWISVRMDEVKLKPSFIKNAFAFEGPGEILSDFE
                     KKKVNVEGKIKGNVVNGNVNITTSRIEIEGIGNLKFSGFLSNWGKEKFEGVIELNGVR
                     VKEILEMTKYKIPFDGKIYGKVFIEKEKEKIKEIKFNLDIKEISQANYQSQFNLYVKG
                     KYLPLEKKGLLEEGVLKNEKGEQLVFSGFISENEFEFIFDTKEFSLDEFLKLLPEEII
                     KKYNLKMEGSKFSSNKFVLNFLKKNLF"
     gene            19597..20199
                     /locus_tag="PLF90_01855"
     CDS             19597..20199
                     /locus_tag="PLF90_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56063.1"
                     /translation="MNYGKLIIENINGKFNKDLEGDIKFSFYKGKGNIDIKNKDFSYI
                     NGKLFIENLNLKKLAYSFNEKIVFEGLMNINGEFNIGKGKFEIDVTFNSVKKRGIKQF
                     MNFGAIELIASLSGGNPIRMAGSSNFYYKDIKGRISIKDNRFTIEGLLGEKNGNQYLI
                     TKPFLLPGINILINKKSNTIEIHELINRVNLAIERIREKD"
     gene            complement(20201..21001)
                     /locus_tag="PLF90_01860"
     CDS             complement(20201..21001)
                     /locus_tag="PLF90_01860"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOK56064.1"
                     /translation="MKEILKINPERVRDFLVEFLRDEFKKANKGVAVFGMSGGVDSTV
                     VGYLCKMAFGRNNTYGIILPYKNTPKISVDHAYMVGRTLGIRYLRFNVTPQIDYYFKN
                     FPQADRIRIGNKISRERMSILYDLSSHFDGIVVGSSNKSEIYLGYGTLFGDLAWLINP
                     LGDLFKTEIYQLAQFLGIPEEILKKKPSAELWEGQTDEEELGFTYQDADIILYYYVEK
                     NMKVEEITGQIKNLSPEIINKVIEKVKKTEFKRKAPKIAKIPDEIKYS"
     gene            complement(21053..21124)
                     /locus_tag="PLF90_01865"
     tRNA            complement(21053..21124)
                     /locus_tag="PLF90_01865"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21089..21091),aa:Glu,seq:ctc)
     gene            21171..22223
                     /locus_tag="PLF90_01870"
     CDS             21171..22223
                     /locus_tag="PLF90_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56065.1"
                     /translation="MSQKSIYNEVVGVIHVHFPKRRVKEYIKIISEDGKKAKLDFIIL
                     TSHTPLKGKEKYKELFEIDGYYNDILIIHAEEVDEKKKNHFILIGNKKWTKEEEIENV
                     LKENEKFIKLIVHPYGKHRLFFVKKDYKWEKWDSVFHGIEIWSSLFDWADKTRIYNIP
                     LRYLNFPFNIDVPAEKILKKWDELNMKKKTSGFAGLDIHSLPFYFKILDFKKNFSYKN
                     VFRTLRNHIYLREKLSGNLEEDKVKILTSIKNGNLFFANDYIQNSSNFYFGEKDGKYI
                     CGEEGKVNDIILIKNPVKAKTKLIRNGKVIMEGIIEREEIKIEKEGNYRVEVFLDNRN
                     WIFSNNIYITPASSHL"
     gene            22331..22492
                     /locus_tag="PLF90_01875"
     CDS             22331..22492
                     /locus_tag="PLF90_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HOK56066.1"
                     /translation="MGKKKYKDTVCRKWLTKETKNVVEKDGKIYYFCSPSCKEKFEKD
                     PEKYLKLVG"
     gene            22492..23331
                     /locus_tag="PLF90_01880"
     CDS             22492..23331
                     /locus_tag="PLF90_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOK56067.1"
                     /translation="MKIKINDLIVLDIETTGLNPDKDRICEVSFLKIKKDNLIENFTT
                     LINPEIKIPESVSLISGIKDVDVENAPKFKEIVEKIRNFINGEILLCHNASFDISFLK
                     KEFERVGYQFPEVKIIDTYLIAKNFFSFRKNSLHYLSQFYKLKRISEHRAEDDAKATF
                     EIFKIFCREIEKRYRPNSLNLLFLFRYQKKFFYRRIIFDINQIENKIYPDRIILEDIL
                     KALSEKKEILIRYMNFKKEITERKILPLEIIDENGVKYLKAFCQLKKEERKFRIDRIK
                     KIM"
     gene            23462..23668
                     /locus_tag="PLF90_01885"
     CDS             23462..23668
                     /locus_tag="PLF90_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOK56068.1"
                     /translation="MAKEKGKVKWFNNKKGFGFIERENGEDVFVHYSSIVGQGYKTLN
                     EGDAVEFEVVKRDKGLQAVNVVKI"
     gene            23686..25317
                     /locus_tag="PLF90_01890"
     CDS             23686..25317
                     /locus_tag="PLF90_01890"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK56069.1"
                     /translation="MLKKHIFLYLLLTLIGIGETGKDYLKLAITSSQNGFYELSNKYI
                     EDYIAGGNQDYVDYVYLLYGYNLLKLEKYHQAIEKLETVIKRFPLSQYLKNAYEFLIP
                     AYLKTEDVDSSINSYIEYKNKFGPDEKIEKQISEKIIEKGINLFKNKNIQEARTYFSL
                     ILNEFKDVDLTLWANYYLGLCEFESGNFIKAKDYFEKVIISGKGDIVSDSKLKTGDCY
                     FNLEKYEEAENYYKKLLEENSIFSQWAKFQIAMIEKKRGNLSKSIEILNNIELKDDSN
                     LIFNVLNEKANIYILLEKWEKAEETLNLLINKFPERKEIPEIYFKIGIINFNKKDFDK
                     SIFYFKKAIELANKNSVKEKGLFFAGYVNYLKGNFNDCLNFWENLIKEYPESPFILQI
                     SYLKGKKFYQDNNLKEAEKNFKEIVEKNNNPYYEEGLTYLIEVLIKEKKFEEGEIYIK
                     KFLENKYDNYIEFLLGKIYYLKEEYTKAKDIFENLKIDSPVIKAEMNFYLGKIYQKQG
                     NIEKAKEKFIEVISLYPQFKEWKEKSEKSLKELKK"
     gene            25272..26711
                     /locus_tag="PLF90_01895"
     CDS             25272..26711
                     /locus_tag="PLF90_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56070.1"
                     /translation="MERKIRKVIERIKEIVLTLIFLFPLFTYSQNIDIYEEIEGKDLT
                     EKKTKIFYIMPSITDINFPQIPEVKIEEETKVETEITETKEIIEEKKIEAEKGKKKWF
                     FNGKSGNFVTRGFDIVYNDEKMNFGISNFYTENYRKNSGFFYTRFYLSSSPNLFNLDF
                     ITGQMELPGPVKNPFEDKREFFYLNWDFKKRFSYFNFGFSHKFYAIDEEISHFLGFNM
                     EKDFKSFKLNTEIEEDIFDNESVFSITEYIIFEKENFLIKPGLKFIEGSGLRFLPSFL
                     FKINENFSVFLSSDFEIPDLWKDVIMENWKEIKKEKVLPEEIYKGGVKFQFKDTEFEI
                     SHSYNKKYLWEDIANNNLYQPFREKFEKTGFLLNSKIPLKDNLNLFLEIEKDIFDKKI
                     SFLPEYKHNAGIEFKNEKINFKIWNSFTGERHFIKEDLKSFSTLNFEITYRTKFEIGF
                     GIYNILNEEYEIAPDYPGEGRKFLIYFKF"
     gene            26851..27531
                     /locus_tag="PLF90_01900"
     CDS             26851..27531
                     /locus_tag="PLF90_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOK56071.1"
                     /translation="MKRKGFTLIELLVVIAIIAILAAMLLPALARAREQARRGVCVSN
                     LKQLGLALHMYAQDYDERFPTNIDKNAPTGHDVAVSLQLLTGQLNESDALKEGVVYVK
                     DPGVFVCPSSAETKSDTGLICQRTCSYVYALPLHEQTAQETAIMADRWARSLSTANSA
                     LYAIESTTYDLETQDNHGTEGINVLYVSGNVKWVASYKKSGNYYLPTSELPNCTNGST
                     TALRIPRP"
     gene            27581..28312
                     /locus_tag="PLF90_01905"
     CDS             27581..28312
                     /locus_tag="PLF90_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOK56072.1"
                     /translation="MRKRGFTLIELLVVVAIIAILAAMLLPALARARETARRGVCVSN
                     LKQIGLALKMYAQDYDENFPDVSGAAAPPGQTGEDGNVIMAFNKLLGYTNSTVGNWQN
                     IRPCPNYLKDPGIFICPSQKNDGKSQNVRITKKEECSYAYAMDSGWSYPLNEQTQDDS
                     VIVVDKQTLAAILSLGNKMWNTTNVAIALKLSPDNNHSTAGVNALFVGGAVNWIPSVK
                     QGTDYYIPSSGDYKGIPNWMNIRNP"
     gene            28374..29093
                     /locus_tag="PLF90_01910"
     CDS             28374..29093
                     /locus_tag="PLF90_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOK56073.1"
                     /translation="MRKKGFTLIELLVVIAIIAILAAMLLPALARAREQARRSVCISN
                     LKQIGLALRMYSQDYREFFPITSATTLQTAVGCLGLLYPNYVSAQKTFICPSDISHRT
                     VDLAGSEITQGISTQLRDTTGDTDGAGLSYAYALWCHENVDIDTVLVVDKAVYITDYQ
                     GGTYTAGTPWSLTALQSGATSTSGGASAVPVGLLNHKNDGVNACYIDGHVRWIPVGKV
                     EELFPNYNNADNSQAKVRNPL"
     gene            complement(29169..>30025)
                     /locus_tag="PLF90_01915"
     CDS             complement(29169..>30025)
                     /locus_tag="PLF90_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK56074.1"
                     /translation="LRNYDWLDPISFYEKSLKYFPYSKKLIHNYGVELLMKYKYEKAL
                     IQFKKLEKQFANSKRELSKATLGISKSYFMLGNYEEAMKYYIKTLEIDPFNFDALTGL
                     GLIYERKGDTENCLNFLNKAVQINPYSSPTLYLLGKIYLDKGNYDLAEKIFIKAIELD
                     PKESIYWNKLGICYFKEGQFDKSLYCFLNAYKFNKNDFLIILNLARACQRLNKHNEAI
                     FYFERVFELEPEKTKKNIEILNDYAISLNEIGEKEKSIEILKKILKIKPDFEPAKINL
                     RIILQQKK"
BASE COUNT        10791 a         4072 c         4536 g        10626 t
ORIGIN      
        1 tgtaacaata ttcaggaatt aacaaaaagt tatttgcagg acacaattgg cattgaaaga
       61 atatcaaata tagaaatcta tgtttcaaat ataataggtg agtttgaaaa caaagaggaa
      121 aatgaaaaac cagaagaaac ttgagtttct ggaaaaagag gttaaaaacc acattgaact
      181 tgtaaaaatt gttttctctc cttcttttaa tgaaaatctt ataaaaattt ctgaaactat
      241 tgttgattgt ataaaaaatg gtaataaaat tcttctatgc ggtaatggag gaagcagtgc
      301 tgattgtcag catttttgtg gtgagatggt caatagattt aaaaaagaaa gagaaccact
      361 cccttttatt tctttgacga cagatacttc tgtaattaca agtataggaa atgattactc
      421 ttttgaagat atattttcaa aacaggttaa agcaattgga aaagaaaaag atattttaat
      481 ctgtttttca acttcaggtg aatctaaaaa tgtaataaaa gcagcaaaag ttgcaaaaga
      541 aaaaaaaatg aaggttattt cacttacagg gaaaacacca aatacccttg aaaaaatatc
      601 cgactttatc atctctatcc agtcaaaaga aacagaaaag gttcagcaaa tacatctaat
      661 tatctatcac ctgctttgct atcttattga ggaagaattt tgaaaatgtg taaaataaaa
      721 actcttaaag aaataaaaaa aatttctgaa agattgaaga aagaaggtaa aaccattgtt
      781 tttacaaatg gttgttttga tataattcat ccaggacata taaagatttt gaaaaaagca
      841 aaatcaatgg gagatgtttt aatagtcggt cttaatagtg ataaatcaat taaaaaaatt
      901 aaaggagaaa aaagaccaat aatagaccag agaggcaggt gtgaaattat gtcttctttc
      961 tggatggtgg attatattgt tttatttgaa gaaaaaacac ctgaaaaact gataaaatca
     1021 attttaccga attttattgt aaaaggtagt gattacaggg aagaagaagt tattggcagg
     1081 gatattattg aaaaatatgg tggaaaggtt gttattgttc cattaaataa gaaatattca
     1141 acaacaaatc tgataaggaa aataaatgaa aattttaagg attattgatg taaattttaa
     1201 cagggtcaga gaagggatta gagttattga agaatctgta agatttatat gtgaggataa
     1261 tgaaatttta aagaaaataa aagaaatcag acataaattt tcaaaaggcc tcttatacta
     1321 ttttccatct gataaattaa aaagttatag atttgtggag acagacatag gaaagacatc
     1381 tgatataaaa cagaaattga atttaaaagg attgatagaa actaactttt tcagagtgga
     1441 agaatcttta agagtccttg aagagtattc aaaagtactg aatcctgata gtttttcttt
     1501 ttttcatgat ttgaggtttg aagtttaccg attagagaaa aaagttatta ccagaatttc
     1561 aagaaaaaaa ataaaaattc catctctata tgtaatactt aatcttaaag aaaatgaaaa
     1621 agaattttta aaatttgctg aaaaagtgat taaaggaaag ccagatataa tccaactaag
     1681 atataaagga gaaaattcaa agtttttttt aaaaatagca aaaaccttga aaaaaattat
     1741 tcctgatgag ataatatatt taattaatga cagagtagat atatgttttt tatgtgaagg
     1801 tgatggagtt catttaggaa aaaatgatct tgatgtagaa agtgtcagaa aaatattacc
     1861 tgataaaatt gtaggtgttt caacagggaa tatagaagat gtaaaagaag ttaaaaatgg
     1921 agatatagat tatattgcga ttggttctat ttttaaatct ccgacaaaac cagaaaaaaa
     1981 acctgttgga aaagatattt taagaagaat aaaagaaatt atatctatac ctcttatagg
     2041 gattgggggt ataaatatag aaaatgcagg ggaagttatt gaaaaaggag cggatggagt
     2101 ggctgttata agtgttgtgg aaaattcaga aaatcctgaa aaaataataa aaaagttaaa
     2161 ggaggagata aaaaaaggat ggaagaaaaa gggaaaaaga ataatttgaa ttttttgaaa
     2221 ggttttttat tctgggttct ggttataatt tttatttttg ctcttttgaa attttctgaa
     2281 gttgaaggga aaaagataaa cttaatttat agtgatttca taaatgaggt taaaaaaggt
     2341 aatattgaag gtgtattgtc tataaaaaag aatatagttt acggaaaatt aaaggatgga
     2401 agaaattttt atacttttat tggagaagac cctgatttaa acagaatttt aattgataat
     2461 aatatttctt ttaaatatga gccaggtaac tggatagcag aactcattcc gtatattatt
     2521 cctgctttga ttttcttttt tattttctgg atgtttataa aacaggcgaa tattgaaaga
     2581 aatagggtta tgtcttttgc aagaagtact cctataattc caaatccaag aaatagagtt
     2641 acttttgaag atgtggctgg atgtgaggaa gcaaaggaag agttaaaaga aataattgaa
     2701 tttttgaaaa atccaaaaaa atttcagaaa ctcggtggta aaattccaaa aggagttctt
     2761 ttaataggtc ctcctgggac aggtaaaact ctccttgcaa aagcagttgc aggagaagca
     2821 ggagttcctt ttttaagtat gagtggttct gattttgttg aaatgtttgt tggagttggt
     2881 gcagcaaggg taagagacct ttttagacag gcaaaacggc ttgctccatg tattgtgttt
     2941 attgatgaaa ttgatgctgt tggaagacag agatttgctg ggcttggagg aggacatgat
     3001 gaaagagaac agacattgaa tcaactactt gttgaaatgg atggatttca aacggatgaa
     3061 ggtataattg ttatggctgc gacaaataga cccgatgttt tagaccctgc tttaacaagg
     3121 cctggaagat ttgacagaac agtggttgtt acacttcctg atgttaaggc aagggaaaaa
     3181 attttaaaag ttcatacaaa aaataaaccg cttgatagtt ctgttgacct ttctttaata
     3241 gcaagagcaa caccgggact ttcaggagct gaccttgcaa atattgttaa tgaagcagca
     3301 cttcttgcag caaaaaaagg aaaagaaaaa attgatatga gtgattttga ggaagcaaag
     3361 gataaggttt taatgggagt tgaaaggaaa agtttatatg taagtgaaga ggagaaaaaa
     3421 attattgctt atcatgaggc aggtcatact cttgttcaga aaagtttgcc agaggtatat
     3481 cctgttcata aagtaacaat tattccaaga ggacaggcac ttggaataac tcatatactt
     3541 cccgataagg ataggtttct tgaaagtgat acatactata aaaattcact tgcagcactt
     3601 cttgcaggaa gggcaacaga agaaattgtt tttggcagga aatttactgg tagtgaaaat
     3661 gatttaaggg tagcaacaga gattgcaaaa aaaatggtat gtgagtgggg aatgagcgaa
     3721 aatttaggtc ctataagttt cagaccccat gaggcagttt ttcttggtag agaccttgtt
     3781 cagcagagag aattcagtga agagacatca agagaaattg ataaggagat aaagaagatt
     3841 cttgaagaag cagaggcaag agcaaaagaa ataataatga aaaataggga taaacttgat
     3901 attcttgtta aagaattgct tgaaaaagaa acattgacat cagaagatat tgataaaatt
     3961 cttgggatag aaaaaggaga ggaaaatgga catagtgaaa atagaaaagg ggatggaact
     4021 gataatagaa gcactgggta aaaatataga caagaaaaaa cttaaagata caccaaaaag
     4081 agttgcaaag atgtacgaag agattttcag tggaatagat gttgacccta aaaaagttct
     4141 ggggaaggtc ttcaaggagg attttaatga acttgtactt gttaaagata taaattttta
     4201 ttcaatatgt gaacaccact ttcttccatt tttcgggaaa gcacatgttg cctatttacc
     4261 ggatggtaat aatgttgttg gaataagtaa aattgcccgt cttgttgatg tactttcaaa
     4321 aagacctcaa ttacaggaaa gaatgacaaa tcagatagtg gatataatta tggatgtttt
     4381 aacacctcag ggtgcaatgg caataattga agcagaacat atgtgtatga taatgagagg
     4441 gataaaaaaa ccagggacaa aaatagtaac aagtgctatg agaggtatat ttttaagaga
     4501 tttaagaaca agaactgaag cactcaattt gatttctcca tcaatccgtt tttgattatt
     4561 tcataaatgg agaaagaagg ataacaatcc ctgctatcat caaaataatt ccagcaactg
     4621 ctataatgtc agaagtggat tccttttcat atgtcaacct tggtttaaat gttaaaaaaa
     4681 ttccacccag taataataaa aaacccagaa ttataaatat acctttcatt tttatttatt
     4741 ctcctttttc ttttataatt tttccagata gaaaattttt taaaattttt tcagcagtta
     4801 cagaaataat ttcatttttt aaatttttat cagaattaac tattacagga atataatttt
     4861 ctgaatatcc tttcaagaag ttcccagttt tttcttcaat caaaacttca agttttttac
     4921 caataaaatt ttctttaact tttttgcttg aataatttga aatttttaaa attttcttaa
     4981 atctttcttt ttttgttttt tcctctactt tatcaggaag atttattgca actgtatttt
     5041 ttctttctga atagggaaaa atatgaactt ttaaaaattt aattttatca atagccatac
     5101 aagttttttc aaaatcttcc tctttttccc caggaaatcc aactattacg tctgttgtga
     5161 aagtcaccct atctatattt tccttaatca tatcaatctt ttcaagataa tcgttaaaag
     5221 tatatcttct tcccattaaa tttaaaatcc tatctgaacc actttgaaga ggaatatgaa
     5281 aatgcggaca gaatttattt atttttttta aagaattgat aaaaaattca ttaatataat
     5341 aaatttcaat tgaactcaac ctaatccttt ttatttcttt tatttcttct attttttcaa
     5401 gaagggaaag aagtttttca cctgtatctt ttccataaga accaaggtca atccctgtaa
     5461 gaacaatttc tttatatccg ttttctccaa gtttttttat ttcctcaatt atatcttttt
     5521 cttttctgct tttaaccttt cctctaacaa aaggaacaat gcagtaagta cagaaatttt
     5581 cacatccatc ttctattttt acaaaggcac gagtatgttc atcaaaacgt tcaattttat
     5641 ctttgaactt taagaaaaaa tctctttcaa caaaatttat attttcaagt tctcttagtt
     5701 taccattttt tttgataaga catcctgtta aatatatttt ttttccttct tttaacaatt
     5761 tttttatttt tttctttact tctttttcta ctttttcagt aacacagcat gagtttaaaa
     5821 taacaatatt ttctgattta aaattgtcat ccttctctat attttctttt atgagttggc
     5881 tttcatattg gttaacttta caaccaaaag taataacctt tgccatttta tatttcttct
     5941 atttcaagac gagcaagtaa ataggaaatg gtagattctg ccccttgatt tctatttggt
     6001 cctgattttg aaagtccatc ataacaacca cctgtaacag ggtcatacat catttcacct
     6061 ttagtatttc ttccaaggaa ccagtcaaat acaattaaag ataagtttcc atatttttca
     6121 tctccggtca atttttctgc tttttttaat gcctcaatca tacatgccgc ttcaattggt
     6181 tgctggtcat aatatgctct tttcccacct tttatgtacc agccattatt tcctataggg
     6241 ataaatctgt tttctatgat tgtaaggtca ataagaaaat tgagagtttc catcccaata
     6301 tcaaaatatt ttttatcctc aagtatctca taggcataaa gcaaaccaca tggtaattta
     6361 gcattttcat aagttattat gttttcaaac catttccagt ttggttcgct tgaattctgg
     6421 taattttcaa ttattctttc agcaagtttt tttgcaatct ctcttactct ttcattattt
     6481 tcggttttta agtagtagta aagtcctgcc agacaattag ccattcccct taatgaattg
     6541 atgttttcaa tctggggtaa ggcaagatca aatatgtgtt ttgctgcacc tttaatattt
     6601 tcgtagataa aatcacttga aagaagaaac ccaatagccc atatacatct accaaaacaa
     6661 tcatctcctc catctgtatc aagaaagttc ctatcatagc cgagtaaatt atgaaatctt
     6721 ccatttgatt tttgcatgta ataaataaaa ctcagatata tatttatgag atttaaactt
     6781 ttctggtcta tacacatatt ataatgtttt gttacaacca caagtcctct tgcattatca
     6841 tctatggtgt aacctgtttt tctatcagcc atagaaaatt tagcatgctg tattattcca
     6901 acatcatctg taagtgtttc aagatgggtt agttttattg gaagcaatct atcctcaaaa
     6961 taaatagaag cactttctcc tctactggaa ctgactcttg aaaacaaagt taaatattcc
     7021 catgcaactt tttcccatgt cattttttgt ccgagtttat atgcttctct ttctatctgc
     7081 ttcttttttt ctggattttt caagagataa aggactgtat cactaatact ttcagggtcc
     7141 ctgaaattca caattaaccc tcttttacca ttttcaaaaa tatcttttgc ataaagataa
     7201 ggggttgaaa tacatgcctt accgcatccg attgcatagg acagagttcc acttgtaatc
     7261 tgtccagggt ttagatatgg agttatataa atatcagttg ctccaagata atcaagcaat
     7321 tcttcaagag ttaaaaattt attcacaaat tttacattat taacaatatt caatctctca
     7381 cattctttaa tcagaaattc cctataactt tctccttcct gttttactat atttgggtgt
     7441 gtagcaccta ttattaaata tacaacatca ggaaattctt ttaaaatcga tggaagagaa
     7501 tatattacat attcaagtcc ttttcccctg cttaaaagtc caaaagttga aaggactatt
     7561 ttattattta aatttaattt ttctttaaaa taattggttg gccttaaata agaaaaaggg
     7621 acaccatgat aaattaattc tatctttctt tttgcaattc cataaaaatc atttaaaagt
     7681 tcttttgcga tatttgtcat cacaacaata tatgaacttt tttcggatat ctttctcaca
     7741 atttctctca ttttctgggg tggatttggt agtactgtat gaaatgttgt tacacagggt
     7801 tttttgagat gttcaagaaa ttcaagaaga tactctccat actctccacc aaatatacca
     7861 aattcatgct gtatcccaac aatttcaatt tcagattcgt ttacaaattc acctgctttt
     7921 ctgtaatcct ctatttcttc aatagtaata attctgacaa cttcttcagg ataattataa
     7981 gtgtcgtctt ttccgtttaa ggcaataact tttgatggaa ttaaaatatt atagttgtca
     8041 atatgtctta ataaatcatt tgtaaaagta gcaattccac atggttttgg tatataagtt
     8101 gaaagaaaac agatttttgg tttatatata ctatactcag ttggtttaaa aaatctcgtt
     8161 tttgttacta tttccatttt tattccgaaa tttttgaaaa ttgatttata taagaattat
     8221 cgcctattgc agaattttta actaaacaat tatttctgat tataacagaa tctccaatta
     8281 tagaattttc tatgatacac tcatctccaa taaatacatt tccaaaaatt atggagtttt
     8341 caataataca atttttccct ataaatgatt ttttacctat aataaccttt cccttaaata
     8401 ttgaattttc accaattata acattttcat ctgcaatcaa cttcccttca acgattacat
     8461 tatttccaat taaaacatta tctttttttt ctatattttt ttctgatttt ttgtaaagaa
     8521 gttcaatttt tccatcaatt aaatcaaaat tccctttttt atatttatca attgttccga
     8581 catcaagcca gtatccataa tgaatatatc catacatatc tttacctttt tgaagaattt
     8641 gagggaatgt ctctttttca accgaaactt ctttattttc tggtatttct tcaagaactt
     8701 ctggttgaaa aatataaaca cctgcattta ctgtatcagt aataatttct tctttttttg
     8761 gtttttcaat gaatttaatt attttcatat catcatcagt taaaattaaa ccataagatg
     8821 aagggtctgg aacttttatc atccctattg ttataaatgc attcttttct ttatgaaaat
     8881 ttaagatttt ctcaagatta aaatctgcaa ggacgtctcc gttaaacaca aaaaatgggt
     8941 ctttcccttt taaaaacttt tcagcattct ttattccacc acctgtccca agtggttttt
     9001 tttcatatga taaataaacc ttaattccca tctgttttcc aatctctatt gcttttttaa
     9061 aatcatcccc tttataaccc actccgagaa caacatctac tattccatag tttttcaata
     9121 attcaaattg ataacttata aaaggataat ttacaattgg taaaagcgct tttggtgttg
     9181 ttattgtaaa aggtcttaat cttgtaccga atccccctaa aagcaaaagc gccttcatac
     9241 ttatctcctt tttgatttta aaatgaattt atcatttgag aacatatata tattttaccc
     9301 tattatctct tttttcgcaa agaaaaagac atgaaaattt gagaaatgaa aattgaacag
     9361 tatttttaaa tctgaactat attttttatt gtcttttcta tctctttata atcaaatggt
     9421 tttatgaaga tataatcagc accctcttct tttgcttttt ttatattttc ttcttccgga
     9481 tgacctgtga taacgataat aacaatattt ttcaaattag ggtcattttt tataatattt
     9541 aaaacctcaa atccattaat accaggcata aatatatcaa gtattaaaat ttctggttta
     9601 aaagaataca gatattttcc aacttcaaat ccattataag cagttattat tctgaaattt
     9661 gtatttcttc tttcaaggaa ttcttttaaa gaatttgata tatcaacatt atcatctcca
     9721 attaaaatct ttacttcttt ttctgtttca tatcctaaaa gttttctctc tttcattatc
     9781 ctttctactt ctttttttaa aatcacccat cttccaccag gtaatctaac tccttcaatt
     9841 tttttatcta ttatccattt ataaacagta attctgctca ctccaattat ttttccaagt
     9901 tctcctgttg tataaaattt cttttccata ttaaaccacc tcatcaaaaa ttgtataata
     9961 aaaatctttt tctttttgtt cctgttgttt ctttattctt ttaccattaa ttaaaatata
    10021 gggcagtgct tttctcaatc taacccctat ttttttcaat gtgatttctg attttatttt
    10081 tccttctttc cttgcttcca aaatcttttt tgctgttttt attccaaccc cgggaatgcg
    10141 aattaattga tataaatctg ctttgttgat ttctacaggg aaaaaatctt tattcctttc
    10201 agcccagaga atttttgggt ctctatttaa atacaaattt ccatttctgt caaaaggaat
    10261 ttcagaaggt agaaatttat attttcttat aagaatatct gcctgataca atcttacctg
    10321 tctttttaag gagcagggct ttttattttc aagaggtgtt tttaaaacag gaataaatcc
    10381 gctatagtaa actcttgaaa gattataatt tttataaaga taaaaggaaa aacttaatat
    10441 ttctctatct ttttcttcag aagaaccaat aataatctgt gttgtaatcc cattctttaa
    10501 aggactttct ttgtttaggt ctgaaagttt ttttaaggtt aaaagtaaat tttttgaaaa
    10561 gtccttttct tttgctatcc ttttcatata ttctgaatta actgtttcca tgtttaaaga
    10621 caatctatca gcataaattt tccccttttt tattaaatat tcgtcaactc ccggtagaat
    10681 tttaagatgt atgtaatcat tatatttata tttttttctt aaaatttcca caacttctaa
    10741 cattttttcc tgcgtttcat tattatcccc atttattgca gaggatagaa aaagtccgtt
    10801 tacaatccct tttctccaca tagaataaaa tatatctgca agttctctta cattaaaact
    10861 ataccttgga caatctctat ctcttctatt ggcacagtaa agacagttaa atttacagtt
    10921 atttgtaaga agaactttga aaagttttac aatcctattt ccacatacag aaggatatat
    10981 aaaagatgta aacctttctt tttgatttat atattcactt acttcaaaaa ttccaagatt
    11041 tcctaaaaaa tttaactttt cttgcatatc cttaattttt attatacaat ttttaagatt
    11101 tgagagaaag ggaaaatgaa cgagagagaa agatataaaa aaactctatt atttgagaaa
    11161 gtagataaaa ttcctttttc acctggtagt ccgagagaat caactttaaa aagatggcat
    11221 aaagaagggc ttcctgaaaa tgttaactgg tttgagtata tatgtgaaaa aataggaata
    11281 aagatagaaa aaccaaaaaa aacaaaaatt aatttatcct attcgttttt cttgaatcct
    11341 ctatttgaag aaaaggtaat agaaaagaaa aatggacact taattgtaca gacggagaaa
    11401 ggagagattg tagaaattga tgaaaaatac gatttcacat atttaaggag cgcaaaggat
    11461 tttgtgacaa gaaaatggca caaatttcct gttgaaaatt taagcgattg ggaggatatg
    11521 aagaaaagat ataatcctga gagtcctgaa agaatagacc ctgaaattga taaaaaagca
    11581 gaaattttaa aagaaaggga ctgggtttta ggaatatcat tccatggacc tttctggcag
    11641 ttaagagagt ggtgtggatt tgaaaattta tgtattttta tggcagaaaa ggaggatttt
    11701 gttcttgaaa tggctaattt ctggaaaaat tttgtttctg aaattttaaa aaaactaatt
    11761 ttaaaagttc agtttgacta tgctgttata aatgaagata tggcttataa agaaaaaagt
    11821 atgatttctc cctcaatgat taagaaatat ttaatgccct gctgggttga atggacatct
    11881 ttattgaaaa aaagtgggtg tgaagtagtt atggttgatt cagatggcta tattgaagag
    11941 ataataccca tatggataga aagtggaatt aactgcacaa taccagttga aattgctgca
    12001 ggaaatgacc ctgttaaatt cagaaaaaaa tttggtaaga atatggctta tgttggagga
    12061 attgataaaa gaaaaattgc aaaaggtgga gatgttttaa aaaaagaaat ggacagaatc
    12121 ataccatttt tatttaaaga tggaggttat ataccttcct gtgaccatgg tgttcctcct
    12181 gatatatctt ttgaaaattt tattgagtat tgtaagtatc ttgcaaaata tacaggatac
    12241 ctttaaaatt caaaattcat tccatcatta gcaattataa cttctgttcc aaccctttca
    12301 cttatttggt cttttttttc ccatatttta ttttttagca ttgtcattcc aaaatgtgtt
    12361 agaattgctt tttttggttt tatatttttt attatttctt ctgcttcgga taaacataag
    12421 tgctgtatat tttctttttt ttcataaagg actgtattga taataagtat ttcactgttt
    12481 ttataataat caattaattc agggaaaaaa gatgtatccg gcaaatatga aataacagta
    12541 tttttcaagt ttattttgaa tccgtaagta tcaacaccat gcctgagttt caccggtatt
    12601 tcaaaattta accctttaag tttataatct ttcttttcct ctaaaatttc aatcctttca
    12661 acaaaatccc tgcaatataa aagaataaca gggtcgtgat acagacaatc atatggagca
    12721 aaaacaactc cccttttttt aaatccacca tctgtcatcg cttcaatcat aaggtttatt
    12781 tcgcatgaat ggtcaagatg tctgtgagaa agaaaaattc cttcaagatt cacaggattt
    12841 aattttggtt ttgacttcac cgctcttaat aaagttcctg gtccagggtc aacaagaaac
    12901 tgtatccctt caagagaaaa ccagataccc cctgaatacc tcaattgtga aatcataaca
    12961 aatcttgcac ctgctgttcc gagaaatttg atagaattca tttaataaat gactttgaac
    13021 cgaatattcc gtgggtaaat atttgcaatt tttttccttt ataagaattt ctcaatttct
    13081 gtattgcctt tgcttctatc tgtctaatcc tttccctcgt taaattgaaa atctgtccga
    13141 cttcttcaag agttcttgga taaccttctc catcaagtcc aaatcttaat cttattatca
    13201 gttcttctct ttcatcaaga attgagagtg ctttttctat ttcttctttt aaaagagaat
    13261 gtcttgtata tgacaatgga tttggattga atttgtcttc tataaagtca cctatagttg
    13321 ctctttcttc atctccaata ggagagtcaa tagaaattgg gtctggtctc attgattttt
    13381 ttatattttt tattttttct acaggtaaat tcatttccct tgctatttct tcttccgaca
    13441 gttcattttc ttctccaagt tgtgaatact ttgcctcaat tttttttact ttatttattt
    13501 tttctatcat atgaacagga attcttatag tagaagaatg gtctgcgatt gctcttgtta
    13561 ttgcctgtct tatccaccat gttgcataag tactgaactt aaaaccagtt ttgtatttga
    13621 atttctccac cgccttcata agtcctatat ttccttcctg aattaagtca agtatagaaa
    13681 gttttggatt gctgtatctt ttggctatat ttataacaag tcgcagattt gaatttatca
    13741 atttttctct cagtttattt aattgctttt catatttttc tatttgtttt tttgaaggat
    13801 ttgatttttt acttaattta tttaattgtt tttttgcttc ttctatccct tttgcaattt
    13861 caatttcttc ttcatgtgtt aataaattat aacttcctgc acctttaaga taagacctta
    13921 atggatttct tgcatcatct atatcaatct ttttttcttt ttccacaaaa aatacatctt
    13981 cttcctcttc atcttcaagt atgtcaatat tataatattc aagttcatca aaaagttcat
    14041 ttatatcctc ttcatctgtc atatccattg gaagaatttc gtttatatca tcccatgaaa
    14101 gatacttttt cctttttcca agttcaacta tttcatttat tttatcttca atttttgtag
    14161 cttctttatt tttaacactt ttcttctttc ttcccatatc tcactccttt tttaatttat
    14221 ataacaattt ttgcatttct tttaaatcat ccttataatc aatccctttt tccagttttt
    14281 cattcatttt tttcttgtat gactcaattt ctatcaacaa ttttttcttt tttatttttt
    14341 ttaaacaatc attgaaaatt ttttccttta actcctctga aattttatta ttatctttta
    14401 tcgcaataga ggaaataaaa cttgcaagtt cttcatcgtt acattcaccc attaaaagag
    14461 aaggatttat ttcaagtttt ttattaatta atttttcacc cgctgtaatt attttctcta
    14521 ttttttctgt aagttgtcct ttaaattcaa aaagtttttc aaaatatttt ttatcattta
    14581 taactatttc tattaaaaat ttttcagcag aattttgatt ttcctgattt atttttgaga
    14641 gagttatata ttccgtttca atatcttttt ctatcatcgc atcaagataa ccttcaagta
    14701 tctcaatctt tatttccatc ttttcagaaa gtttttttaa aaattcatat ctttcaattt
    14761 catccgggac ttttgagata aatttcaagg tttcttttat aattgcagat ttttccttgg
    14821 gatttttaat gtcataaatc tgtgaattta tagatactga gaaatcaaca aaatcctgtg
    14881 aattttcaat aaaatttttg agtgatttta ttccatattc atcaataaat ttatcagggt
    14941 caaaacctga aggaagcata cagattttaa tttcaaatcc cttttttatt attgtttcaa
    15001 ggtttctcaa taccgctctt attccagggt catcaccatc aaaaagcaat aaaattttat
    15061 cagtgagttt tttcatcaaa tttaaatgaa gttctgttat ggtagttccc ataggagcaa
    15121 ctgtattttg cattcctttt atatgcattt tcaaaacatc aaaatatccc tcaacaaaaa
    15181 taacaaatcc ttttgatttt atattttcct taccccagtt tattccatac agacaattac
    15241 ttttattaaa aacaatattt tctcttatat ttaaatattt cggcataatt tcatcatcta
    15301 tggttcttcc tccaaatcca ataatttcgt ttttaatatt ataaattggg aaaattattc
    15361 tatttttgaa tgtgtcttct tttccttctt catcaagtaa agcagcaagt aaaaagtcct
    15421 ttttattcaa tttttcttct tccaattttt ttatgagata attatttgaa ggagcaaaac
    15481 caattgaaaa tttattaatc tgttcatctg ttaaacctct ctgatataaa tattcaagag
    15541 caatttttcc ataagtagaa aaaagagttt tattatataa attggctgca tattcatttg
    15601 cttttaaaat tttctttttt tcaatacttt cttcaatatt ttcttttttt gaaatttcta
    15661 ttcctgcctt ttttgcaaga atatttaagg catcataaaa agatattttt tctatattca
    15721 ttaagaattt caaggcattt ccaccaatcc cgcatccaaa acaatgaaaa acctgttttt
    15781 caggactcac aaaaaaagaa ggtgtttttt cgggatgaaa aggacacaat gctttaaaat
    15841 ttttacccgc tttcttcaaa tttatgtatt cagaaacaat ttcaactata tctatttttt
    15901 caagtatttc ttcaacttca gaacctaaag ttccaccttc cattttatat gtagccctcc
    15961 agaaaaaaaa tatttaaata gttataccca ttagacaatt aaattgtcaa ggtttataat
    16021 ataaactatg tttaaactca tcagatattt tatacttatc gggatagttt tttttactgg
    16081 ttatattttt gggattaaag atgttaaatt tttaaaaata atatcctctt caatggaacc
    16141 taccttaagt attggggata aaataatttc tgtaaaatca aatgatataa aaagaaaaga
    16201 tatagttgtt ttactttctc cggaaggaga aaaagagttt ttaattaaaa gagttatagg
    16261 gcttccggga gaaaaaataa aaattgaaaa gggttatgtt tatataaatg gagaaaaact
    16321 tcaagaacct tatattaaag aaagacctga atatttattt aaagaaatag aaatccctcc
    16381 aggaacttat tttttacttg gagacaacag aaatgaaagt gaagacagta ctacatgggg
    16441 acccgttgaa aaaaacttta ttgttggaaa agttctttgt agatatcatc ctttcagaaa
    16501 atttaaaatc ttttaaaata aaaaaggcgg accggatttt ctaaggtccg cctttaagaa
    16561 ttaaaaatta cttgactgcc tttttcaatt cgcttcctgc tctgaatact ggaaccttct
    16621 tctttggaat ctttatctct tctcctgttc ttggatttct tccttttctt gcttttcttt
    16681 ccacaacttt gaatgttccg aagccaacaa gagtaactac atcccctttc ttaagagcac
    16741 ctttaattgc atctaaaact gcatcaactg ctgctgccgc ttcttttttt gtacaaacaa
    16801 cctttgctac tgcttcaatc aaatctcctt tattcatact ttctcacctc ctttctttct
    16861 tttttattga atttacaaca ctttttaaat tttgtcaaga ggaatttttt atttgtcaac
    16921 aatttcaaaa ttttcaaatt tcaaaatcct tgttattcat cattcttctt cactttttaa
    16981 taagaaaatc aggtctatca atcttgttgc acttttttcc atttcaagta ttagattgtt
    17041 tacacgggat acaaaataat tgtaagaaag ataagcagga attgctacaa caagtccacc
    17101 tacagttgtt acaagcgcct cccagattcc tcttgcaagt tctccaggat ttacaagtcc
    17161 ttctttcatt tcaattacca taaatgcttt aatcattcct gttacagttc caagaagtcc
    17221 taaaagagga gatattactg caatggtcgc aagaattaca agatattttt caagttctgg
    17281 aatttggtgg tttgctgtgt catcaatcgc ttcttttatt tcatcccttg ttctatcaag
    17341 ttttaaaaga cctgctttta taatatttgg aactggttta ttttgatttt cacatttttc
    17401 aattgcttct cttatttttc ttctttttaa aaggtcttct atatctttta taaatttctc
    17461 actgtcaatt tgagaatttt ttaagtaaat aaatctttct attattattg caagagttaa
    17521 aacaccacat ataaagatgg gtatcattat atacccacct ttaacagcaa gtaaccacag
    17581 attcattttt ttcccttttt gtcagagata taataccatc tgaaaaaaaa cttatcaaat
    17641 tttaaaaaaa gtggtaagat taaaaaaaag aaggaaaatt tatagaaact taaaaaaata
    17701 taaggggggt ggaaaatgaa aaaaaatgtt ttaatttctg caatttctgt aataatagtt
    17761 ttggttttta tttcaggttg tggtaagaaa aaagaggaag aacttactcc tttaaatgct
    17821 cctttaaagt atggagaagt aatgagtaag accatgaaaa aggcaaaggc aatggatggt
    17881 gttatttatt tgaaaaataa aattaatact tttcagattc aggaaggaag atatccttat
    17941 tctcttgatg aacttgttga aaaaggatat attaaaaaag aggaaatgcc aaaaccacct
    18001 gaaggaatga agtttgttta caatcctaaa acaggagaaa taacggttaa ataaaaggag
    18061 gaaggtatgg aattttatga agttattaaa acaagaagga gtgtgagaaa ctataaaaaa
    18121 gattccattc ccgatgatgt cttaaaaaga gttttaaatg cagcaagaat agcgcccagt
    18181 gggaataata gacagccatg gaaatttatt attataaagg accgtaagat aaaagaaaaa
    18241 atgattgccc tttgcgatgg acaggaattt gttgctgaat ctcctgtttt aatagttgcc
    18301 tgtggtaaaa atattcatta taatagaggt agatggatgg gtgattacag tgttatcgtt
    18361 gatgtggcaa ttgctgttga ccatcttaca cttgcagcaa gagcagaagg acttggtaca
    18421 tgctggattg gttcttttga caatgaaggg ataaaaaaat ttcttggaat tccggaggat
    18481 gtaaatgttg tcgctctgac acctttagga tatccggaaa atccaaatgt ttttaaagaa
    18541 acaaaaaaca gaaaaagttt agaagaaata atatgttatg ataaatggga ggaaaaatag
    18601 aaaggaaaaa atttaagaag ataaaatact ttattattcc atttttaata atattgtttt
    18661 tcattttctc tttcatagtt tttaaaatta atctgataaa aaatataaat ctcactgaaa
    18721 aatggaagat aaaagatata aatttttcat caggagaaat aaatttaaaa gaccttacct
    18781 ataaagaaac atggatttct gtcaggatgg acgaagtgaa attaaaacca tcctttataa
    18841 aaaatgcttt tgcttttgaa ggtcctggag aaatcctgtc tgattttgaa aagaaaaaag
    18901 taaatgttga aggaaagata aaaggaaatg tcgttaatgg gaatgtaaat attacaacat
    18961 caagaattga aatagaaggt attggtaatt taaaattttc tggatttctt tcaaactggg
    19021 gaaaggaaaa atttgaagga gttattgaac tcaacggagt gagggttaaa gaaattctgg
    19081 aaatgacaaa atacaaaata ccttttgacg gtaaaatata tggaaaggtt tttattgaaa
    19141 aggaaaaaga aaaaataaaa gaaatcaaat ttaatcttga tataaaagaa atttctcagg
    19201 caaactatca gagccaattt aatttatatg tcaaggggaa atatttacct ttagaaaaaa
    19261 aaggactgtt agaagagggt gttcttaaaa atgaaaaagg agaacaactt gtattcagtg
    19321 gttttatttc tgaaaatgag tttgaattta tttttgatac aaaagaattt tcgcttgatg
    19381 aatttttgaa actacttcct gaagaaataa taaaaaaata taatcttaaa atggagggtt
    19441 ctaaattctc ttcaaataaa tttgtgttaa actttttaaa aaaaaattta ttttgacgga
    19501 gaatctctgt tcatttcaaa aattttttct ttcaaagata taaaatttac aaatcttaaa
    19561 tttaaaacac ctgagaattt tttttcctgt gaaaatatga attatggaaa acttataatt
    19621 gaaaacatta atggtaaatt taataaagat ttagagggag atataaaatt ttctttttat
    19681 aaagggaaag ggaacattga tattaaaaat aaagattttt cttatataaa tggtaaactt
    19741 tttattgaaa atttaaactt aaaaaaactt gcttattctt ttaatgaaaa aattgtattt
    19801 gaagggttaa tgaatatcaa tggagaattt aatataggca aaggaaaatt tgaaattgat
    19861 gtaactttta attcagtgaa aaaaagaggt attaaacaat ttatgaattt tggagcaatt
    19921 gaactcattg cctctttaag tggaggtaat ccaatcagaa tggctggaag ttcaaacttt
    19981 tattataaag atataaaggg aaggatttca ataaaggata atcgttttac aattgaagga
    20041 cttcttggag aaaaaaacgg gaatcagtat ttaattacaa aacctttttt acttccagga
    20101 attaatatcc ttataaataa aaaaagcaat acaatagaaa ttcatgaact tataaacaga
    20161 gtcaaccttg caatagaaag aataagagaa aaagattgat ttatgagtat ttaatttcgt
    20221 ctggtatctt tgcaattttt ggtgcttttc ttttaaattc agtttttttg accttttcaa
    20281 taactttatt tataatttca ggacttaaat ttttaatctg acctgtaatt tcctcaacct
    20341 tcatattttt ttcaacataa tagtaaagaa ttatatctgc atcctgataa gtaaaaccga
    20401 gttcttcttc atctgtctgt ccttcccaca attcagcact tggctttttt ttcaatattt
    20461 cttctggaat tccgagaaat tgagcgagtt gataaatttc tgttttaaaa aggtctccga
    20521 gtggatttat aagccatgca aggtctccaa aaagagttcc ataaccaaga tatatttcac
    20581 ttttattact tgacccgaca acgataccat caaaatggga agataaatcg taaagtatgc
    20641 tcattctttc tcttgaaatt ttgtttccta ttcttattct gtctgcctga gggaaatttt
    20701 tgaaataata gtcaatctga ggtgttacat taaatcgtaa atatcttatt ccaagagttc
    20761 ttcccaccat ataggcatgg tcaacagaaa tttttggtgt atttttgtag ggtaaaatta
    20821 ttccataggt attatttctt ccaaaagcca ttttacacaa atatcccaca acagtagaat
    20881 caactccacc agacatgcca aaaactgcta ctcccttatt tgctttttta aattcatctc
    20941 ttaaaaattc aacaagaaaa tccctcactc tttcaggatt aattttaagt atttctttca
    21001 taattcgcct cctttttctg aataatttcc ggttatccag aagttgtttt tttagcccca
    21061 acgggattcg aacccgtacc tgatggctga gaaccacctg tccttaccat tagacgatgg
    21121 ggccatataa tctaattcca tataatctaa ttatggtata atacaattaa atgagccaaa
    21181 agtcaatata taatgaggtt gttggagtta ttcatgtgca ttttcctaaa agaagagtaa
    21241 aagaatatat taaaataatt tcagaagatg ggaaaaaagc aaaacttgat tttattattc
    21301 ttacctctca cactccttta aagggaaaag aaaaatataa ggaattattt gaaattgatg
    21361 gatactataa tgatattcta attattcatg ctgaagaggt ggatgaaaag aaaaaaaacc
    21421 attttatact tattgggaat aaaaaatgga caaaagaaga agaaattgaa aatgtcctta
    21481 aagaaaatga aaaattcata aaactaattg ttcatcctta tggaaaacat cgtctttttt
    21541 ttgttaaaaa ggattataaa tgggaaaaat gggactctgt ttttcatgga attgaaatat
    21601 ggtcatctct ttttgattgg gcagataaaa caagaattta caatattcct ttaagatact
    21661 taaatttccc ttttaatatt gatgttcctg ctgaaaaaat tttaaaaaaa tgggatgaat
    21721 taaatatgaa aaagaaaact tctggttttg caggtcttga tattcattct ttaccttttt
    21781 attttaaaat tcttgatttc aaaaaaaatt tttcatataa aaatgttttc agaacactga
    21841 gaaaccacat atatttaaga gaaaaacttt ctggaaattt agaggaagat aaggtaaaaa
    21901 tattaacttc aataaagaat ggtaatttat tttttgctaa tgattacata cagaatagtt
    21961 ccaactttta ttttggtgaa aaagatggaa agtatatatg cggagaagaa ggaaaagtca
    22021 atgatataat attgataaaa aatcctgtta aagcaaagac aaaattgata agaaatggta
    22081 aagtaattat ggaagggata attgaaagag aagagattaa gattgaaaaa gaaggaaatt
    22141 atagagttga agtttttctt gataacagaa actggatttt ttcaaacaac atatatataa
    22201 caccggcctc atcccacctt taaggtggga aggtggaatt gttgataatc aagggttttg
    22261 aatgccgttt tttaaaaatt aagaaaagat gatataatat ataaaattta tggtctaata
    22321 ggagtgaaaa atgggaaaga aaaaatataa agatactgtt tgcaggaaat ggcttacaaa
    22381 agaaacaaaa aatgtagtgg aaaaagatgg aaagatatat tatttctgca gtccttcctg
    22441 taaagaaaaa tttgaaaaag accctgaaaa atatttaaaa cttgttggat aatgaagatt
    22501 aaaataaatg atttgattgt acttgacatt gaaacaacag gacttaatcc tgataaagat
    22561 agaatttgtg aagtttcttt tttaaaaata aaaaaagata atctaattga aaattttacc
    22621 actttgataa atccagaaat aaaaatacct gaaagtgttt ccttaataag tggaataaag
    22681 gatgtagatg ttgaaaatgc tcctaaattt aaagaaattg ttgaaaaaat tagaaatttt
    22741 ataaatggag aaattttatt atgtcataat gcaagttttg atatatcatt tttaaaaaag
    22801 gaatttgaaa gagtaggata tcaatttcca gaagtaaaga ttattgatac atatttaatc
    22861 gcaaagaatt ttttttcctt cagaaaaaat tcccttcatt atctttcaca attttataag
    22921 ttaaaaagaa tatcagagca tagagcagaa gatgatgcaa aagcaacatt tgaaattttt
    22981 aaaatttttt gtagagaaat tgagaaaaga tacaggccca attcattaaa tttacttttt
    23041 ctgttcagat atcagaaaaa atttttttac agaagaataa tatttgacat aaatcagatt
    23101 gagaataaaa tatatcctga tagaattatt ttagaagata ttttaaaagc gttgagtgaa
    23161 aaaaaagaga ttttaataag gtatatgaat tttaaaaaag aaattacaga aaggaaaatt
    23221 ttacctcttg aaattataga tgaaaatgga gtaaaatatt tgaaagcatt ctgtcagttg
    23281 aaaaaagaag aaagaaaatt tagaattgac agaataaaaa aaattatgta aaataataaa
    23341 agttaaagga ggattttagg gaaggagttt ttaaattcca atccgtaaaa tctaaaaaaa
    23401 attaccccgg agatttaaaa ttattttaaa ttttctggga tgagaaagga gggaagaaag
    23461 agtggcaaaa gaaaaaggaa aagtaaaatg gtttaataac aagaaggggt tcggcttcat
    23521 tgagagagag aatggagaag atgtatttgt ccattacagt tcaattgtag gtcagggtta
    23581 taaaaccctt aatgaaggtg atgcagttga atttgaagtt gtaaaaagag ataaaggtct
    23641 tcaggcagtt aatgttgtga aaatataata taaaaagagt cctgaatgtt gaagaaacat
    23701 atctttttat atcttctcct gacattgatt gggatagggg aaaccggtaa agattatcta
    23761 aaacttgcaa ttacatcttc acagaatggc ttttatgagt tgtcaaataa gtatatagaa
    23821 gattatattg caggtggaaa tcaagattat gttgattatg tctatctcct ttatggctat
    23881 aatctcctta aacttgaaaa atatcatcag gcaatagaaa aacttgagac agtaataaaa
    23941 aggtttcccc tatctcaata tcttaaaaat gcatatgagt ttttaattcc cgcctattta
    24001 aagacagaag atgttgattc aagcataaat tcatacatag agtataaaaa taaatttgga
    24061 cctgatgaga aaattgaaaa acagatatct gaaaaaataa ttgaaaaggg aataaacctc
    24121 tttaaaaaca aaaatattca agaagcaaga acctattttt ctttaatctt aaatgaattt
    24181 aaagatgtag atttaacttt atgggcaaat tactatcttg gactctgtga atttgaatca
    24241 ggcaatttta taaaagcaaa agattatttt gaaaaagtga ttatttcagg gaagggagat
    24301 attgtttctg atagtaaact taaaacaggt gattgttatt ttaatcttga gaaatatgaa
    24361 gaagcggaaa attattataa aaaactattg gaagaaaatt caattttttc gcaatgggca
    24421 aaatttcaga ttgcaatgat agagaaaaaa aggggaaatc tttcaaaatc aattgagatt
    24481 ttaaataata ttgaacttaa agatgattca aatttaatat ttaatgtttt aaatgaaaag
    24541 gcaaatatct atattttact tgaaaaatgg gaaaaagcag aagaaacctt aaatttactc
    24601 attaataaat ttccagaaag aaaagaaatt cctgaaatat attttaaaat aggtattata
    24661 aacttcaata aaaaggattt tgataagagt attttttatt ttaaaaaagc aatagaactt
    24721 gcaaataaga attcagttaa agaaaaagga cttttttttg caggttatgt gaattatctt
    24781 aaaggtaatt tcaatgattg tcttaatttc tgggaaaatt tgataaaaga atatcctgaa
    24841 agtccattta ttttacagat ttcatatctt aaaggtaaaa aattttacca ggataataat
    24901 ttaaaagagg cggaaaaaaa ttttaaagag atagtggaaa aaaataacaa tccttattat
    24961 gaagaaggtc taacatatct aatagaagtt cttataaaag aaaaaaaatt tgaagaaggt
    25021 gaaatttata taaaaaaatt tcttgaaaat aaatatgata actatattga atttttgctc
    25081 ggtaaaattt attacttgaa agaagaatac acaaaagcga aagatatttt tgaaaattta
    25141 aaaatagata gtcctgttat taaagcagaa atgaattttt atcttggtaa aatctatcag
    25201 aaacagggaa atattgaaaa ggcaaaagag aaatttatag aagtaataag tttatatcct
    25261 caatttaaag aatggaaaga aaaatcagaa aagtcattga aagaattaaa gaaatagttc
    25321 taactttaat ttttctcttt cctctgttta catattctca gaatatagac atttatgaag
    25381 aaattgaagg gaaagacctt acagagaaaa aaacaaagat attttatatt atgccttcaa
    25441 ttacagatat aaattttcca caaatacctg aagttaaaat agaagaagaa acaaaagtag
    25501 aaacagaaat aacagaaaca aaggaaataa ttgaagaaaa aaagatagaa gcagagaagg
    25561 gaaaaaagaa atggtttttt aatggtaaat ctggaaattt tgtaacaagg ggttttgata
    25621 ttgtttacaa tgatgaaaaa atgaatttcg gtatttcaaa cttttatact gaaaattaca
    25681 gaaaaaacag cggttttttc tacacaagat tctatttatc ttcatcccct aatcttttca
    25741 atcttgattt tataacaggg cagatggaac ttccgggacc tgttaaaaac ccttttgaag
    25801 ataaaaggga atttttttat ttaaactggg attttaaaaa gagattttct tattttaatt
    25861 ttggattttc ccataaattt tatgctattg atgaagaaat ttctcatttt ttaggtttta
    25921 atatggaaaa agattttaaa agttttaaat tgaatacaga aattgaagaa gatatatttg
    25981 ataatgaaag tgttttttcc attacagaat atataatttt tgagaaagaa aattttttaa
    26041 ttaaacctgg tttaaaattt attgaaggaa gtgggttgag atttttacct tcttttttat
    26101 ttaaaataaa tgaaaatttt tcagtttttt taagttctga ttttgaaatt ccagatttat
    26161 ggaaagatgt aattatggaa aactggaaag aaataaaaaa agaaaaagtt ttaccagaag
    26221 aaatatataa aggtggagta aagtttcaat ttaaagatac agaatttgaa atttctcatt
    26281 catataacaa aaagtattta tgggaagata ttgctaacaa taatctttac cagcctttta
    26341 gagaaaaatt tgaaaagaca ggttttttat tgaattcaaa aataccactt aaagataact
    26401 taaatctgtt tcttgaaata gaaaaggata tttttgacaa aaagatttct tttcttcctg
    26461 aatataaaca taatgcaggt atagaattta agaatgaaaa aataaatttt aaaatatgga
    26521 attcttttac tggtgaaaga cattttatta aagaagattt aaaaagtttt tccacattaa
    26581 attttgaaat tacttataga actaaatttg aaattggttt tggtatttat aatatactta
    26641 atgaagaata tgaaattgct ccggattatc caggggaagg aagaaagttt ctcatttatt
    26701 ttaaattttg aaaataataa gtaaaaaact tgacaaaatt aaaaatataa ggtaaaaata
    26761 aacttgacaa gattaaaaag atagtgtaca ttaagaatag atgctgcaga aaattaaaat
    26821 gttattaaaa aagaaaggag gtgaaaaaag atgaaaagga aagggtttac attaattgaa
    26881 ttgctggtag taattgctat catagcgatt cttgcagcga tgttactgcc agcacttgca
    26941 agagcaagag aacaggcaag aagaggtgta tgtgtgagta atttaaaaca acttggactt
    27001 gctttacata tgtatgctca ggattatgat gagagatttc caacaaacat tgataaaaat
    27061 gcaccaacag gtcatgatgt ggctgtttct ttacaacttc ttacaggtca gttaaatgaa
    27121 tcagatgccc taaaggaagg agttgtttat gtgaaagacc ctggtgtatt tgtatgtcca
    27181 tcttcagcag aaacaaaatc agacacagga ttaatatgcc agagaacttg ttcctatgtt
    27241 tatgctttgc ctttacatga acaaacagca caggaaacag caattatggc agatagatgg
    27301 gcaaggtctc tttcaacagc aaactctgct ctttatgcta tagaaagtac aacttatgac
    27361 cttgaaacac aggataatca tggaacagaa ggaattaatg ttttatatgt aagcggtaat
    27421 gtaaaatggg ttgcttctta taagaaaagt ggaaattatt atttaccaac atctgaatta
    27481 cctaactgta caaatggttc aacaactgct ttgagaatac cgagaccata aaatgaaaaa
    27541 ttattgtttt gaaaatttgg aagaaaggag gtgaaaaaaa atgagaaaaa gaggatttac
    27601 attgattgaa ttgctggtag tagttgctat catagcgatt cttgcagcga tgcttttacc
    27661 agcacttgca cgtgctcgtg agacagcaag aagaggtgta tgtgtgagta atttaaaaca
    27721 gatagggctt gctcttaaaa tgtatgccca ggattatgat gaaaattttc cagatgtaag
    27781 tggtgctgct gctcctcctg ggcaaactgg tgaagacggt aatgtgatta tggcttttaa
    27841 taaattactc ggatatacaa attctactgt gggaaactgg caaaatataa gaccatgtcc
    27901 aaactatttg aaagaccctg gtatttttat atgtccgagc cagaaaaatg atggaaaatc
    27961 acaaaatgta agaataacaa aaaaagaaga atgttcctat gcctatgcaa tggattctgg
    28021 ttggagctat cctttgaatg aacaaacaca ggatgatagt gtaattgttg ttgataagca
    28081 gacattagca gcgattttat ctcttggtaa taagatgtgg aatactacaa atgttgctat
    28141 tgctttaaaa ttatctcctg ataataacca ttcaactgca ggagtaaatg ctttatttgt
    28201 tggaggtgct gtaaactgga ttccttctgt taaacaaggg actgattatt atataccttc
    28261 ttcaggtgat tataaaggaa taccgaactg gatgaatata aggaatccat aaaaacttga
    28321 caaacaggag gaagtatggt aaaattaaaa ataaagaaag gaggtgaaaa aagatgagaa
    28381 aaaaaggatt tacattaatt gaattgctgg tggttatagc gatcatagcg attctcgcag
    28441 cgatgttact gccagcactt gcaagagcaa gggaacaggc aagaaggagt gtatgtataa
    28501 gtaacttaaa acagattggt cttgcattaa gaatgtatag ccaggactac agagaattct
    28561 tcccgattac atcagcaaca actcttcaga cagcagttgg atgccttgga ctactctatc
    28621 caaactatgt ttcagcacag aaaacattta tatgcccatc tgacatttcc cacagaacag
    28681 ttgaccttgc aggttctgaa attacccagg ggataagcac acagttaaga gatacaacag
    28741 gagatacaga tggagcaggt ctttcttatg catatgcttt atggtgccat gagaatgttg
    28801 atatagatac agttcttgtg gttgataagg cagtttatat tacagattat cagggaggaa
    28861 catatacagc aggaacacct tggagtttaa cagcattgca aagtggagca acttcaacaa
    28921 gtggtggagc aagtgctgtt cctgttggac ttctcaacca caagaacgat ggagttaatg
    28981 cctgctacat agatgggcat gtaagatgga taccagtagg aaaagttgaa gaactgttcc
    29041 caaattacaa caatgctgac aattctcagg caaaagtaag aaatccatta taatctgaaa
    29101 agaggaagcc cccgctttta aagcgggggc ttttttattt ttatataaca tttatcagaa
    29161 ttttcaattc attttttctg ttgtaatata atccttaaat taatttttgc tggttcaaaa
    29221 tctggcttta tttttaaaat ttttttaagt atttctattg atttttcttt ttctcctatc
    29281 tcatttaaag aaattgcata atcgtttaaa atttcaatat ttttttttgt tttttctggc
    29341 tctaattcaa aaactctttc aaaataaaaa attgcttcat tatgtttgtt caatctctgg
    29401 catgctcttg ctaaatttaa tattattaaa aaatcatttt tattaaattt atatgcattt
    29461 aagaaacaat ataaagattt atcaaattgc ccttctttaa aataacaaat gccaagttta
    29521 ttccagtaaa tactttcttt tgggtcaagt tcaattgctt ttataaaaat tttttctgct
    29581 aaatcataat tacctttatc aagataaatt ttaccaagaa gatatagggt aggagaagaa
    29641 tatggattta tttgtactgc cttgtttaaa aagtttagac aattttcagt atctcctttt
    29701 ctttcatata ttagtccaag acctgttaaa gcatcaaagt taaaagggtc aatctcaagt
    29761 gttttaatat aatatttcat tgcttcttca taattaccca gcataaaata acttttactt
    29821 attccaaggg ttgcttttga aagttctctt tttgaatttg caaattgttt ttccagtttt
    29881 ttaaactgaa taagtgcttt ttcatattta tatttcataa gcagttcaac tccataattg
    29941 tgaataagtt tttttgagta agggaaatat tttaatgatt tttcataaaa acttatcggg
    30001 tccagccagt cataatttct taaaa
//
LOCUS       DAOSAT010000012        27259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_1283_length_27259_cov_5.783414, whole genome shotgun sequence.
ACCESSION   DAOSAT010000012 DAOSAT010000000
VERSION     DAOSAT010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 27259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27259
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1283_length_27259_cov_5.783414"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1554)
                     /locus_tag="PLF90_01920"
     CDS             complement(109..1554)
                     /locus_tag="PLF90_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56075.1"
                     /translation="MERRFFQMNFADWNNFVFIDEKLVEESSGVKKIFYSLEKYEKNP
                     VIKTEKLWESYLSNFDAGYIYDPFYSSMVYDNEKKIYWCFYQAFGRFGSYLCLAQSED
                     GINWIKPELNQVLFDGSFENNILKFPEYPALSIDFGCVVPNYLKNIDAELICSNFSRF
                     DDNLYFQGITVAFSKDGINWDFHFPPVLPYDGDAHCLMWDPVEEVYIITTRSYQQYNV
                     YRRFGMKRKRHIAISKSKDLIHWTPMITVLEADDKDPENREFYKMFILPYGHGYIGFL
                     QIFDVDPSLSKGPCEVQLVFSRDLLNWDRVGDRKPFIPRGEKDQWDSGMTLMTISPVK
                     EGGNLRFWYGGKKGEHWIGSTGGIGTGVIRKDGFVSWTAEGEGYIVTCPLHISGIPEI
                     FLNLKAPKGEVRVEILEDNKVLPGCEKENCIPVSGDHILAPVRFKTQLLRFGGDVRLK
                     FYLKNAELFSFKGNFTLPERYKYPKRFPNWW"
     gene            complement(1567..>5016)
                     /locus_tag="PLF90_01925"
     CDS             complement(1567..>5016)
                     /locus_tag="PLF90_01925"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOK56076.1"
                     /translation="EIVELNQRMDINFVVFPLATPDKFAVWSGYYKPVPVTNEEYEKA
                     LEEKLKGKYDIIVMGKVRWSVFPQKFRDMILNKVKDEGTILLAYFGDWYGCKLAEDIK
                     LDDLKKATENKINFDRKFLYPYKGLPQFKKYKDFEEFINSTLEVYSYGNGKILLLKGY
                     SVPHFQNLTPASIRSPFETKYIEYDYLLAYIIQLMISYVKSPEIEITGTDYINADINS
                     ISDIKFVLKSNETKKVTCELVLRNEDNEVILKDKKDINLSKGENEVKFEIKNKIPKGN
                     YFADIWVKEKGGIVNFGSSFIEVNSNDYIGEVILNKSYKRNEEITGEVNIFSKDGKDN
                     LKLKISLIDNFGRLEKTEIFDFKGEKINEKKVSFKITPPKAYLTILQWLKFELLSDNN
                     IVDRSLKFFSVSDLRPKDDFRTILWFPPFTGAYTERWYCRNIYEAGFDSRYYTGMGPV
                     AILENLWYVSNPIRFVDMKTDPWGTMDRKPEDHVRQPCLNDLDYLSKVEEDLKKKIEN
                     ELHFSTYEFSCGDECHFASWRPPYDKYELCWCDRCVSKFHEYLEDKYGNIKRLNQEWK
                     TDYTSFDEIKPVTYQEAIENPKLRPLWINYRIFMEDTWTGIFEYAKEIITRIIPNAKV
                     GYEGSQRGYVFTNSFTAEDYYKLSQIMTLNNPYSHPFIRYAFKDFAKEGSLLGLGWQG
                     SYGAGIKNRKIDPWRVLFFGANSLWIWHGPPREQSVVSPDLSFYEYFRPYISQIPELK
                     KGIGKLFISSKREDDGIAILYSNTSVHASALTPQLPPICENLNFFVRYFESSGYQFRI
                     ISYKQLEDGILNKEKFKVLILPYAQSISQKQAKEIISFVNNGGIVIADIRPGVCDENG
                     NFYQNGGVLDNIFGIKQNTEKFEVKKIENFELKYGELACSLPSIHLDSSIILTNGKAK
                     ENVENIPVFIVNNFGKGKGILLNLSLINYPDENMQGIRNILNKVIGEIAGIQRKINYE
                     PDNTEIGFTPYRYKLGSHTYLGILSPWREGEEVRECKLTLPQKFHIYNIRKGEYIGFS
                     DSLNIKVLPEDVLIFGFLPYKINDLKINSIKKVKKGEILNYEIELQTEPQSPETHIFN
                     VSIISPDGNEINYYTENLKGEKGKAKGSVIFALNEKEGEYILRVKEVISGIYKDFQFE
                     ISD"
     assembly_gap    5017..5116
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5117..6291)
                     /locus_tag="PLF90_01930"
     CDS             complement(<5117..6291)
                     /locus_tag="PLF90_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56077.1"
                     /translation="MLVKILFFDYRHIEVIEGFERKIERPEKFKGNPIFVSGHPLEEN
                     WISYYGSVIRRPDGLWQTWYTTIIKDKGILALGYAESEDGIKWERIEKDAIKINNKNT
                     HFVFDREPHGASIIYDEKEERENWKYKLICGAHPSGCISIFHSKDGIHWLPAQKNPVI
                     GTNPDCPMSLLRLPSGKYVAYHRPVLGDRRIARSESWDFINWSEAKIVIDQSPEDPPQ
                     IQFYGMGAFPYGEYEIGTLWIYHTDKNDFGFWKMEGYQEPELTYTRTGYAWHRIEKNR
                     PWIERGKEDDWDCGQIQPASAPVFLEDEIRFYYVGTRATHGKSLKEWDRPEPRCGIGF
                     ASVKVDRFVGIDAKEEGFLITRPFWTEKGEFFINAKIKGYVNVEITDLSGKSISGFE"
     gene            complement(6303..7088)
                     /locus_tag="PLF90_01935"
     CDS             complement(6303..7088)
                     /locus_tag="PLF90_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOK56078.1"
                     /translation="MRRKGFTLIELLVVVAIIAILAGMLLPVLARARENARRVTCMNN
                     LKQIGLATHIYAQDYDGFIPVGGIPTWTCECPTIWRLENATHPGYVGLGILCIGWRQT
                     GRGRYLPDPMFLLCPSVPRYFYWVRKYYHKGWMYQWFEYPNITSNNKVYNNYARNLRP
                     YSDANTYKETKAKVDICAKKGYIWVADSLGYYAWNYGNPPGASSEGYSHSGRDRLPMG
                     LNVLFFDGSVKWINDQDHKLLYKYRCNSYGTESFWTLTANDLK"
     gene            complement(7094..7738)
                     /locus_tag="PLF90_01940"
     CDS             complement(7094..7738)
                     /locus_tag="PLF90_01940"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK56079.1"
                     /translation="MRTKAKELKEKIIEYLLTNFRENGKEKITEEKIASYFKISRTPV
                     REVLKSLENEGLISVRRNSGIKIREFSKEDIKNVYDVRIILEEYAIKEAIKNLKEENI
                     KKLREYVRNYNKARKEGKWLEAKKIDQLFHEKIVELSGNWYLNYLVKKLNVFSFFFSL
                     PKKNQKYRIDPNPYSHNRILKALIKKDHEIASETIKNHILWIKNYTLKNFEKRR"
     gene            7923..8189
                     /locus_tag="PLF90_01945"
     CDS             7923..8189
                     /locus_tag="PLF90_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56080.1"
                     /translation="MRKELEMKVRKAISKTIYNNPKTPYQRVMECENIDEKTKKKLKE
                     VYESLNPVEIKKKLIELEDKLFEMVRRKRLKEKGEKIEKEEIFA"
     gene            complement(8587..8659)
                     /locus_tag="PLF90_01950"
     tRNA            complement(8587..8659)
                     /locus_tag="PLF90_01950"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8624..8626),aa:Val,seq:cac)
     gene            complement(8650..9684)
                     /gene="mtnA"
                     /locus_tag="PLF90_01955"
     CDS             complement(8650..9684)
                     /gene="mtnA"
                     /locus_tag="PLF90_01955"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008731655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOK56081.1"
                     /translation="MEKIPVKPFWWKNGLYIIDQRILPGKEIVLKLDNVKKVYRAIRE
                     LKIRGAPLIGCVSAYGVIVSFLENKKLSFEKIKEKIKKDINFLKSSRPTAYNLFYALN
                     RMERIVDDKSIKDKEELFERLLEEVESIYEEDLNACYKIGEYGNKIIKNGMNILTHCN
                     AGGLATSGFGTALAPVFVAHQKGKKIHVYVDETRPVLQGARLTAWELEKNKISYTLIC
                     DNMAGYLMKKGKIDIVIVGADRIAKNGDTANKIGTYSLAVLANYHKIPFYIAAPFSTF
                     DFNIEDGSEIPIEERNENEVKKINGKYICPLNSNVFNPAFDVTPAYLITGFLTERGIF
                     KPEEIKNLGG"
     gene            complement(9687..13127)
                     /locus_tag="PLF90_01960"
     CDS             complement(9687..13127)
                     /locus_tag="PLF90_01960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOK56082.1"
                     /translation="MKKFVHLHLHTEYSLLDGMCRIEEVVPLAHKYGMNSLAITDHGT
                     MAGIIKFYSSCIESGIKPIIGSEMYVAPKSRFDRKTDEQRSSFHLTLLAKDKDGYKNL
                     MKLSTISYLEGIYYKPRIDKEVLSQYSKGLIGLSGCLQGEIAWYLRNDDFQKAIETAG
                     IYSEIFGKDNFYIEVMLLNMEEEKKIVTLLIELSKKTGIKLVATNDCHYIYKNDKLAH
                     EILLCIQTRTKIDDPNRFKFQTDQIYFKKPEEMIETFKEIPEAIDTTLEISEKCNLTF
                     DFGKYHLPKFTPPSNKTVDEFLEELIKKGLKEKFGIEVDNLEGKSDNEILKRVSYEFK
                     IIKQMGFSGYFLIINDFVNYAKSKGIRVGPGRGSAAGSLIAYLLDITQINPISYNLIF
                     ERFLNPERISLPDIDIDFCDRRREEVIDYIKKKYGENNVCQIGTYGTMQARAVVKDVG
                     RVLNFTFTEADRISKLITHEHETLKQELNENPELKSLKESDEKIKQLFDISVKLEGLA
                     RHSSIHPAGIVITERDVSEYVPLFKGQNGEIATQYEFECIEKIGLLKIDILGLKTLSV
                     IEDTLNLIKERKGIEIKEFPFDDEKTYQLLSKGETIGVFQLESEGMRNLLREIQPQKF
                     EDIIAVLALYRPGPMKSGMVREYIERKKDPSKIKYDHEILEEILSPTYGVILYQEQVM
                     QIANKLANFSMGEADVLRKAMGKKIPSEMEKMREKFVKGAVSNGVKKEIAEKIFENIS
                     NFAGYGFNKSHSAGYALISYQTAYLKANYPLEFMTALLNSEIGNQDKIAEYIEECERM
                     NIWILPPDLCESDEKFKIFGNDIIFGLSAIKNVGSAAIKSILESRKEGDFISLYDFCE
                     RVNLRSVNKKVIESLIKSGAFDYLGIYRSQLFAMIDDAIEHGNKMQKLKDENHVEIFT
                     SKEIKKLTPALNGEAIKSLPEWPQTKLLSYEKEMLGVYLTGHPIEKYVALIKLYSMPF
                     SQIEKIKDGETVIWGGILTDIKRTTTKKKAEKMAIGEIENIDGKIKGLFYPNVFAEFS
                     QFIRANNIIFVKGKLKKDQDEIKIIVDDVATINNVKEKFLRKVEIDLKIPLGDDKFEK
                     VKDLFLRFRGDCPVFINIITNDNKKIKIKPRNFGIDLNEEFIEELKKVIGEDCLHPGI
                     "
     gene            complement(13124..14284)
                     /locus_tag="PLF90_01965"
     CDS             complement(13124..14284)
                     /locus_tag="PLF90_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HOK56083.1"
                     /translation="MAMWIGINRKARVCYGFDDISLVPGTVTLNPEEVDISTQICSYK
                     LDIPVFAAAMDGVVDPKFAVLFGKFGGIAVLNLMGIYTRYNDPYSVIEKIISSSPEES
                     TQIIQNVYKEPVKENLIEERIHEIKKSGVICAVSCIPQYAERYLEIAQSSGVDIFVVQ
                     STVTTVKHISTKYKPLDLYSFCKKAKVPIIAGNCVTFEVAIELMDTGVSGILIGIGPG
                     AACTTREVLGIGVPQVTATSDCAAARDFYFKKTGRYVPIITDGGMVTSGDICKAFACG
                     ADAVMIGSAFVKAVESPGKGYHWGMATSDENLPRGTRIKVGTTGTLKEILFGPAKVDD
                     GSQNLMGALKLSMASLGAKNIKEMQLVEIAIAPSVKTEGKIYQSVKKLQREK"
     gene            complement(14402..15274)
                     /gene="rsmA"
                     /locus_tag="PLF90_01970"
     CDS             complement(14402..15274)
                     /gene="rsmA"
                     /locus_tag="PLF90_01970"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOK56084.1"
                     /translation="MKFLILKEIRKLIETGKIDIKKNLGQHFLIDRNARDKLLSFANL
                     NKRDTVVEIGPGLGSLTEGIIENVKEYYGFEIDDDFCKFLEENFSEYKNFHLIKNDFL
                     KSDENFWNSFKGKIKVIGNTPYYLSSPIVFHILKFYRKIKLALLTVQKEVGERFVGKP
                     GSKQYCPISVLLYFYTDTKICYFLNKNVFYPRPKVESVVVKIVPLKKPRIKIENEKKF
                     YEFLPLIFSYRRKKLTNVVKRVFKIEKDVLVKELSNYEILPQKRVEQLTPEEIFQLFN
                     IIKSIKNSPDKNLV"
     gene            complement(15276..16241)
                     /gene="pdxA"
                     /locus_tag="PLF90_01975"
     CDS             complement(15276..16241)
                     /gene="pdxA"
                     /locus_tag="PLF90_01975"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOK56085.1"
                     /translation="MKKIIGITLGDPAGIGPEILLKGYEKIKNLKNFIPLIIGDIPVI
                     EKNIENLRIELNINEVRKKEDIKEDFFNVYSTGIIKNKNFPVGIDSKICGKASFKYVI
                     DGINLWKKRIIDAIVTLPISKKAWHLAGYNYSGHTELLAERLNERKYAMVMIAENYRV
                     LLLTTHIPLKDVFKYLNERYITEKIKIGYEFLKKLRIKNPKIGVCGLNPHAGENGVLG
                     KEENEILKPVIEKIKKEVINIEGPFPADTIFRKKFDLIIAIYHDQALIPLKTFYFEKL
                     INFTAGIKLLRTSPGHGTAFDIAYKNKGNPESFISAYKFVIKCFE"
     gene            complement(16241..17035)
                     /locus_tag="PLF90_01980"
     CDS             complement(16241..17035)
                     /locus_tag="PLF90_01980"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOK56086.1"
                     /translation="MKRFLIFFFLFVLTVFGEDKIVAIVGNKPVFEKEVVLRSKRDKI
                     DYPIALQVLIEEKLLLYQAEKNKIEVSDEEIKNEIERIKKNFPSLKEFYNYLNERQIK
                     ISQLREEIENSLKIRKLIRNEVISKIEITPVEIANEMKKIEEEYNEYEFFFKWFDNEE
                     DAEKFVRNFNTESLKEMELAKLKSSEIIEEILEKISKMEKEKISFPFKIGEKWIVIYL
                     KEKTHLDADKLEIYREAKDRIFKIKYSLLYRNFIEELKKTIPVKFL"
     gene            complement(17032..17925)
                     /locus_tag="PLF90_01985"
     CDS             complement(17032..17925)
                     /locus_tag="PLF90_01985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOK56087.1"
                     /translation="MKIKVIKMKLFFKKWKGIIGLVIFCLLIFGLIVFLIIKDKRKTV
                     AVVNGYKVTIDEIVEKIESSPEIYKEYFFVDPKSVIDDYVNQILLFQYAKKYERKLRK
                     KVESKIKNYYMEVLTQVFVEDILSDTIKISDEEISNYYNSHLQEFVIPERVQISEIVV
                     DTKEKADDILNRLRLGESFEKIAETESIAPTREKRGEIGWIEVDKLNPEVSSLITQMK
                     PGEILANIIKTEMGYHIIKLTGRTEKRILTLTEATPMIKNLLISQKKKVEVENLMKKL
                     KEKSKVIIYPNKIEILKEKIK"
     gene            complement(17922..20240)
                     /locus_tag="PLF90_01990"
     CDS             complement(17922..20240)
                     /locus_tag="PLF90_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56088.1"
                     /translation="MKRRLKRQLKLDGKKGDVIILVILVLLLIFLFTSTMLVLFNLWA
                     KTTGKIIFGREAKNFSKIGIENAIWEIDKDDREYDCFFDAWRTNFEGDEVDLNNDGEN
                     DSKWFYVKDRKGNIIGRYAVLVEDESGKININASGNLNTSFNEGHSVFEINILEKILG
                     KSIYQNIVSFRYGNDGKPGIVNFDDDNDIIFNPLDGIDNDGNGFIDEPNEGIDEDDEF
                     YFIKPKGDDRPFFSPSEIKMIPGLGEKNYEKIKNFITTFSYDRDLNRKEEKRIDINKA
                     SFYELSEFFKKMGYPEKQAIQIALNIIDFRDTDSIPSVYKTGNKYMTGVEKVPYLNEI
                     DAVKEWKYRELAIGRVFEEEGGQFIEIFNPYPEEIDIGGWRIEGVFVLFSNNWNEVFN
                     TSEEVYDDFVNKETDINQDKAGKITKLVDRFTSITIPSGKKIPPYSFYTIGDNIRIVV
                     IFPNEAPPALLFLPMDGDPDNCQQYERIVALNPGSFGILGDLLAKIPFFAEHGLDFTI
                     KLYDKNGNLIEFCEYFIDGPEVTVQKNDPRMKGISDWVLGPPSPGKLNTYFQPWIGGE
                     FGAANWTIAWPSSFYVKNNQFSTLGELSYIHKMENWKTLNFWKGEDTHILDYLTCYKK
                     VDENVCGRININTACKEVLECLPLVDEKIAERIIDARPFKDISEVIGIYGDKGEKGKL
                     NEEITKYGFDLKDNDLDLFIDIKKEKEMVFSKIVNLITTKSNVFKIISLGQKVQDKNN
                     NGKIEDDEITGVKKMVVWYDRNKKKIIYKREL"
     gene            complement(20200..20826)
                     /locus_tag="PLF90_01995"
     CDS             complement(20200..20826)
                     /locus_tag="PLF90_01995"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HOK56089.1"
                     /translation="MRENTLLPLSEEDRGFTLIEVLIAIFVFTFVCFTAIFILKNSLL
                     SSRKKAVEKDILKEIVFISDFIESRISNAMINDLSGKYRMNFKGGDKWVKFVSPFSED
                     KEGDIAKFGIYWKDNKIMVDMVRIDKDNPDFSFFDGFPGAQILGEDVKDFSIRYFDGK
                     DWQDKWDTEIMEEPVLPEFVEIKIVVSKGKIEGREIEKEIKKTIKVGW"
     gene            complement(20786..21148)
                     /locus_tag="PLF90_02000"
     CDS             complement(20786..21148)
                     /locus_tag="PLF90_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56090.1"
                     /translation="MKYGFTLITVLIGLFILACGIITLIKVYPVIMNLSERSKSSVNI
                     SFIADKIFTQIEQIYGNKDVPLPDNIEGVLNEYPDYKYRVNFNEEKEDLYKVELEILW
                     KKEGKDERKYFVTSLRRR"
     gene            complement(21145..21579)
                     /locus_tag="PLF90_02005"
     CDS             complement(21145..21579)
                     /locus_tag="PLF90_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56091.1"
                     /translation="MGKKGITFLEILIVLFIFLLFLIPVTIFIQKYHQQYLLNSACAE
                     IVEGVNFAREYAINERCSFYVIFNERSFRILKEGKSPVWKEIKMPENIRIKEKTDGVN
                     PLVFLPDGTAKQAGHIILLEEVSKKEKKIKIHNITGKCVIED"
     gene            complement(21584..22795)
                     /locus_tag="PLF90_02010"
     CDS             complement(21584..22795)
                     /locus_tag="PLF90_02010"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOK56092.1"
                     /translation="MALIVQKYGGSSVADIEKIKFVAKKVVETKRKGNKVVVVVSAMG
                     KTTDNLISLAKHIMPNPPEKELDLLLSTGEIVSVALLCMAIESYGEQAEGFPGYFAGI
                     KTDTLHTKARIQKIEPSKIIEELNKDKIVVVAGFQGISSENSITTLGRGGSDLTAIAI
                     SGAIKADICEIYTDVEGVYTADPNRVENAKLISVISYDELLEMASSGAKVMQSRAVEF
                     AKKYKVPFYVKSTFKNTEGTMVKEMELKEGAVVSSVSIDENQAKISIFKVPDKPGIAG
                     KIFSALGEAGINVDVIVQNVGRDNSADISFTVNINDMEKAKNIVEKIGKEIGAEKVIT
                     DSDICKVSIIGIGMMNQPGVAGKMFNCLAKSGINIEMISTSEIKISCVIRKKDGQKAL
                     TQLHKEFIENK"
     gene            22866..24209
                     /locus_tag="PLF90_02015"
     CDS             22866..24209
                     /locus_tag="PLF90_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOK56093.1"
                     /translation="MERNKELEKYKIIWNNIPCIIFILDEKRNILEANEKIKELDYEI
                     EDLIGKNLYDIPSFISKDSLNKLFEKKGEIIKLDVFSGKKEKKIVNAQLIELEEETAR
                     YLIIIHDITEEEQIKTKLYEDEELLKEWFNLAEKSVAGIYIYDEDFNFLYVNPSFCNI
                     TGYTEEEIIGKKKSYELVHPDDVNLSKEMAEKRFKGEIEIASFNIRFKRPDGKIRQCF
                     AIGRVGRYKGKKVITGTLIDITDRVNLENKLKEEHALLEKTLDGTIHAISKIVEIKDP
                     YTAGHQINVAKLSEEISKEIGFNKEKIKEIIYASLLHDIGKISVPTEILVLPRKLTPI
                     EFSIIKTHPIVAYNILKVIPNFENLAEIVLQHHERLDGTGYPRGIKGEKILKEARIIA
                     VSDVVEAMINHRPYRPALSLEEALEEIDNNKGIKYDEEIVDVVIYLFKKKNFSFI"
     gene            complement(24206..24547)
                     /locus_tag="PLF90_02020"
     CDS             complement(24206..24547)
                     /locus_tag="PLF90_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOK56094.1"
                     /translation="MRNIEFGKLGEEKAVEFLRKKGYRIIEKNYRTKIGEIDLIAKRR
                     KEIIFIEVKTRSSDNFGLPQEAVNERKLKKIEQVAQIYLNSKKINLPFRFEVLSIMKS
                     GNDFKFEIIPV"
     gene            complement(24544..24879)
                     /gene="rplS"
                     /locus_tag="PLF90_02025"
     CDS             complement(24544..24879)
                     /gene="rplS"
                     /locus_tag="PLF90_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOK56095.1"
                     /translation="MTLKKVEEIEEKLKPSKEIPEFWPGDEIAVHIKIKEGEKERIQI
                     FTGTCIGRKGKGIRESFTVRKISYGEGVERIFPLYSPNIVKIEVLKTRERNRLAPRAK
                     MYYLRNKNK"
     gene            complement(24860..25567)
                     /gene="trmD"
                     /locus_tag="PLF90_02030"
     CDS             complement(24860..25567)
                     /gene="trmD"
                     /locus_tag="PLF90_02030"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOK56096.1"
                     /translation="MRIDIITIFPEIFTPLDVSIVKRAKKGKIVEIKIWNLRDFSKDK
                     HKKVDDKAYGGGKGMVLKCDIIYDAVEKIKEENREGKVILTSPTGILYNQQIAKKLSE
                     EKGLVIICGHYEGVDERVSKICDYEISIGDYILTGGELPAMVIVDSVVRLLKGVLPED
                     APRYDSFYNYIFDWPCYTRPENFRGMKVPEVLISGNHKEIERWRRKMAIERTKEKRPD
                     LYEKYIKQGGKDDIEKS"
     gene            complement(25574..25816)
                     /gene="rpsP"
                     /locus_tag="PLF90_02035"
     CDS             complement(25574..25816)
                     /gene="rpsP"
                     /locus_tag="PLF90_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOK56097.1"
                     /translation="MATKIRLVRKGKRNRPFFRVVAVSDEKDGRGDVLEILGYYDPLP
                     DPVIFEVKEDRVKYWLNTGAKPSKTVESFLKKKGII"
     gene            complement(25831..26901)
                     /locus_tag="PLF90_02040"
     CDS             complement(25831..26901)
                     /locus_tag="PLF90_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK56098.1"
                     /translation="MIKLGIIGLGKFGTNILNTYSQLEKSGICKLNAICDINEKVLES
                     QVKKFNVKGYPDYREMFEKEELDGVAIATPDPFHKEPTLYAANKKIHVFVEKPLDVTV
                     EGCNEMIEECRKNNVLLQVDFHKRFDPFHQTIKKDIDEGKIGKVEYGSVHMEDKIVVP
                     RDWFPNWAPKSSPVWFLGVHFFDLMRWFLKSNGKNVYAKGIKNKLKSLGIDTFDAVVA
                     TVEFENGAVISFELSWILPENFESIVNQGFKLVGEKGVIECDSQDRGTRVCYQENGVM
                     TYNPVFINEVKKMGHTFYSGYGIESIADFVYNIIHIKNGYKIEDLKRENSITAFGEDG
                     LEVTKIALAVHKSIETQKEEKV"
     gene            complement(26898..>27259)
                     /locus_tag="PLF90_02045"
     CDS             complement(26898..>27259)
                     /locus_tag="PLF90_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOK56099.1"
                     /translation="KSEFEDVIKNCGDDWIVSNYKLSFPKLHNDSYSISPSHILVKTM
                     ERASEETGIVPVVEGFIASCDARLFNRVGKMPVVVFGPGKLDDAHSDHEKIKIDDIKK
                     ASEILIRTVLRIMEVEK"
BASE COUNT         8603 a         5123 c         3086 g        10347 t
ORIGIN      
        1 acagaagaag aaattgaaaa aatgattgaa aaagtccttt tacatgagat tggacattat
       61 ctcggtcttg atgaaaactc attgaaaaaa cttgaccttt aatttcaatt accaccagtt
      121 tggaaatctt tttggatatt tgtatctttc aggaagtgta aaatttcctt taaaactgaa
      181 taattctgca tttttaagat aaaatttcaa tctcacatct cctccaaatc ttaataattg
      241 tgttttaaat ctaacaggtg caagaatatg gtctcctgaa acaggtatac aattttcttt
      301 ttcacatcct ggaagaactt tattatcctc aagtatttca actcttactt ctccttttgg
      361 tgcctttaag tttaaaaata tctcaggtat ccctgatata tgaagagggc aggtaacaat
      421 atatccctct ccttctgctg tccatgatac aaaaccatct tttcttatca ctcctgtccc
      481 tatacctcct gtacttccaa tccagtgttc tccttttttc cctccatacc agaatcttaa
      541 atttcctcct tcttttacag gagaaatagt cataagtgtc atacctgaat cccattggtc
      601 tttttcacct cttggaataa aaggttttct gtctcctact ctatcccaat taagtaaatc
      661 tctgctgaac acaagttgaa cttcacaagg tccttttgat aaagaagggt caacatcaaa
      721 aatctgcaaa aatccaatat atccatgtcc ataaggaaga ataaacattt tgtaaaactc
      781 cctattttca gggtctttat catctgcctc aagaactgtt atcataggag tccagtgtat
      841 taaatcctta cttttactta tcgctatatg tctttttctt ttcatcccaa atcttctata
      901 aacattatat tgttgataag accttgttgt aataatataa acttcttcta cagggtccca
      961 catcaaacag tgtgcatctc catcataggg caaaactggt ggaaagtgaa aatcccagtt
     1021 tatcccatct ttactgaaag caactgtgat accctgaaaa taaagattat catcaaatct
     1081 tgaaaaatta ctgcatataa gttctgcatc tatatttttt aaataattcg ggacaacaca
     1141 tccaaaatca atacttaaag caggatattc tggaaatttt aaaatattat tttcaaaact
     1201 tccatcaaac aatacctgat ttaattcagg ttttatccag tttattccat cttcactctg
     1261 agcaagacag agataagaac caaatcttcc aaaggcctga taaaagcacc agtaaatttt
     1321 tttctcatta tcatatacca tacttgaata aaaagggtcg taaatataac cagcatcaaa
     1381 attagagaga taactttccc ataatttttc tgttttaatt actggatttt tttcatattt
     1441 ctcaagggaa taaaaaattt tttttacacc tgatgattct tcaacaagtt tttcatctat
     1501 aaaaacaaaa ttgttccagt cggcaaaatt catctgaaaa aatcttcttt ccattttttt
     1561 ttcctttcaa tcagaaattt caaattgaaa atccttataa attccagaaa taacctcttt
     1621 aactctcaat atatattccc cttctttttc atttaaagca aaaattacac ttccttttgc
     1681 tttccctttt tctcctttta aattttctgt ataataattt atttcatttc catcaggaga
     1741 aataatactt acattgaaaa tatgtgtctc tggagattgc ggttctgttt gtaattctat
     1801 ttcataattc aatatttccc cttttttaac tttcttgatt gaatttatct tcaaatcatt
     1861 tattttataa ggtaaaaatc caaagatcag tacatcctcc ggtagaactt ttatatttag
     1921 tgaatcactg aatcctatgt actctccttt tcttatatta taaatatgaa atttctgagg
     1981 cagtgttaat ttacattctc ttacttcttc tccttctctc cacggtgaaa gaattccaag
     2041 gtatgtatga gaaccaagtt tatatctata aggagtaaaa ccgatttctg tattatccgg
     2101 ctcataattt atttttctct gaatccccgc aatttcccca atcactttat tgagaatatt
     2161 ccttatccct tgcatatttt catcaggata atttatcaaa cttaaattta ataaaatccc
     2221 tttcccttta ccaaaattgt taactataaa aacaggtata ttttctacat tttctttcgc
     2281 tttaccattt gtcaaaataa tagaagaatc aagatggatt gatggtaaac tgcaagctaa
     2341 ttctccatac ttcaattcaa aattttcaat ttttttcact tcaaattttt cagtgttctg
     2401 ttttattcca aaaatattat caagaacacc tccattttga taaaaatttc cgttttcatc
     2461 acaaactcct ggtcttatat cagcaataac aattcctcca ttattaacaa aagaaataat
     2521 ctcttttgcc tgtttttgtg aaattgattg agcataagga agaattaaaa ctttaaattt
     2581 ctctttattt aaaattccat cctcaagttg tttatatgaa attattctaa attgatagcc
     2641 acttgattca aaatatctca caaaaaagtt caaattttca catattggag gcaattgcgg
     2701 agttaaggca gaagcatgaa cacttgtatt tgaataaagt atagctatcc catcatcctc
     2761 tctttttgaa cttataaata acttgcctat accttttttt aattcaggta tttgacttat
     2821 atatggtctg aagtattcat aaaatgataa atcaggagag acaactgatt gttcccttgg
     2881 aggaccgtgc catatccata aactattggc accaaagaaa agaactctcc atgggtctat
     2941 ttttctattt tttatacctg ctccataaga accctgccat ccaagtccaa gtaaactccc
     3001 ctcctttgca aaatccttaa atgcatatct tataaatgga tgactgtaag gattattcaa
     3061 tgtcataatt tgtgaaagtt tgtaatagtc ctctgctgta aaactgtttg taaaaacata
     3121 tcctctctga cttccttcgt atccaacctt tgcattagga ataatccttg taattatttc
     3181 ctttgcatat tcaaaaatac ctgtccatgt atcctccata aaaatcctat aatttatcca
     3241 taatggtctc aattttggat tttcaattgc ttcttgatat gtaactggct taatttcatc
     3301 aaatgatgta taatctgttt tccattcctg attcaatctc tttatatttc catatttatc
     3361 ttccagatac tcatgaaatt ttgaaacaca tctatcacac caacagagtt catatttatc
     3421 atatggtggc ctccaacttg caaaatgaca ttcatctcca catgaaaatt catatgttga
     3481 aaaatgtaat tcattttcaa tcttcttttt taaatcttcc tctacttttg ataaataatc
     3541 aagatcattc aaacagggtt gtcttacatg gtcttctggt tttctatcca tagttcccca
     3601 tgggtctgtc ttcatatcaa caaaccttat aggatttgaa acataccaaa gattctctaa
     3661 aattgcaaca ggccccatcc ctgtgtaata tcttgaatca aatcctgcct cataaatatt
     3721 tctacaatac catctttcag tgtaagcacc agtaaaaggt ggaaaccata aaattgtcct
     3781 aaaatcatcc tttggtctta aatctgatac agagaaaaac tttaaacttc tatcaacaat
     3841 attattatcg cttaaaagtt caaattttaa ccactgtaga attgtaagat aggcctttgg
     3901 aggagtaatt ttaaaactta cttttttttc atttattttt tctcctttaa aatcaaaaat
     3961 ctctgtcttc tccaatcttc caaaattatc aatcaaactt attttcaact ttaaattatc
     4021 ctttccatct ttactgaata tatttacctc tcctgttatt tcttcatttc ttttataact
     4081 tttatttaat attacctctc ctatataatc gtttgaatta acttctataa aagaagagcc
     4141 aaaattaaca atcccccctt tttcttttac ccatatatcc gcaaaataat taccctttgg
     4201 aatcttattt tttatttcaa acttaacctc attttcacct tttgacagat ttatatcttt
     4261 tttatctttt aaaattacct cattatcttc attcctcaaa acaagctcac atgtaacttt
     4321 tttcgtttca tttgatttca gaacaaactt aatatctgaa atagaattta tatctgcatt
     4381 tatgtaatct gttcctgtta tctcaatttc aggactttta acatatgaaa tcataagttg
     4441 aattatataa gcaagaagat aatcatactc aatatatttt gtttcaaaag gactccttat
     4501 acttgctggt gttaaatttt gaaaatgagg gactgaatat cctttaagaa gtaaaatttt
     4561 tccattccca tatgaataaa cttcaagagt tgaatttata aactcttcaa agtccttgta
     4621 ttttttaaac tgtggaagtc ctttataagg atataaaaat tttctatcaa aatttatctt
     4681 attttctgtt gcttttttta agtcatctaa cttaatatcc tctgcaagtt tacatccata
     4741 ccagtcacca aaataagcaa gaagtattgt cccctcatct tttaccttat ttaatatcat
     4801 atccctaaat ttctgaggaa aaacactcca tcttactttt cccataacta tgatatcgta
     4861 tttacccttt aatttctcct caagggcttt ctcatattct tcattggtaa caggaacagg
     4921 tttataataa cctgaccaaa ctgcaaattt atcaggagtt gcaagaggaa aaacaacaaa
     4981 attaatatcc attctctgat ttaattcaac tatttcnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnatct caaatccaga aattgatttc cctgataaat cagtaatttc
     5161 aacatttaca taccctttaa tctttgcatt tataaaaaat tctccttttt ctgtccaaaa
     5221 aggtcttgtt attaaaaaac cttcctcttt tgcatctatt ccaacaaatc tgtccacttt
     5281 tacacttgca aatccaattc cacatcttgg ttctggtcta tcccattctt tcaaactttt
     5341 tccatgtgtt gctcttgttc caacataata aaaccttatt tcatcttcaa gaaaaacagg
     5401 tgcacttgct ggttgaatct gtccacaatc ccaatcatct tctttccctc tttcaatcca
     5461 tggtctattc ttttctattc tgtgccatgc ataacctgtt ctcgtatagg taagttccgg
     5521 ctcctgatat ccttccattt tccagaatcc aaaatcattt ttgtctgtat gatatatcca
     5581 taaagtccca atttcatatt ctccataagg aaaagcaccc atcccataaa actgtatctg
     5641 tggtgggtct tcaggtgatt ggtctataac tatttttgct tcagaccagt taataaaatc
     5701 ccacgattca gaccttgcta tccttctatc tccaaggact ggacgatgat atgcaacata
     5761 tttacctgaa ggtagcctta aaagagacat cggacaatca ggatttgttc ctataacagg
     5821 atttttttga gcaggaagcc agtgaattcc atctttactg tgaaaaatag aaatacatcc
     5881 ggaaggatga gcaccacata tcagtttata tttccagttt tctctctcct ctttttcatc
     5941 gtaaataata cttgctccat gtggttctct atcaaaaaca aaatgtgtat ttttattatt
     6001 tattttaatc gcatctttct caatcctttc ccattttatt ccatcttcac tttcagcata
     6061 accaagtgca agaattcctt tatccttaat tattgttgta taccaggtct gccataatcc
     6121 atcaggtctt cttataacag aaccataata agaaatccag ttttcttcaa gaggatggcc
     6181 cgaaacaaaa ataggattac ctttaaattt ctcaggtctt tcaatcttcc tttcaaatcc
     6241 ttctattact tctatgtgtc tataatcaaa aaaaagtatt ttaacgagca attttcttcc
     6301 ttttacttta aatcatttgc tgttaaagtc cagaaactct ctgttccata ggaattgcac
     6361 cgatatttat aaaggagttt atgatcttga tcatttatcc attttacaga gccatcaaaa
     6421 aacaaaacat ttaatcccat tggcaatcta tctcttcctg aatgactgta tccttcagaa
     6481 ctcgcaccgg gtggatttcc ataattccag gcataataac ccagagaatc agcaacccaa
     6541 atatatccct ttttagcaca tatatcaacc ttcgcttttg tttctttata tgtatttgca
     6601 tctgaatatg gtcttaaatt tcttgcataa ttattgtaaa ccttattgtt ggaagtaata
     6661 tttggatact caaaccattg gtacatccat cctttatgat aatattttct cacccagtaa
     6721 aaatatcttg gaacactcgg acagagaaga aacatagggt ctggtaaata tcttcctctt
     6781 cctgtctgtc tccatccaat acacaaaatc ccaagtccaa catatccagg gtgagttgca
     6841 ttttccagtc tccatattgt tgggcattca caggtccatg ttggaattcc accaacaggt
     6901 ataaacccat cataatcctg agcataaata tgtgttgcaa gtccaatttg ttttaagttg
     6961 ttcatacagg taacccttct tgcattttct cttgctcttg ccagtacagg aagtaacatc
     7021 cctgcaagta tcgctattat cgccactaca accaacaact ctatcaatgt aaatcctttt
     7081 cttctcattt tcctcacctc cttttttcaa aatttttcaa tgtataattc tttatccata
     7141 agatatgatt ttttattgtt tcagatgcaa tttcatggtc tttttttatc agtgctttta
     7201 aaattctatt atgtgaatat ggattagggt ctattctata tttctggttt tttttaggca
     7261 aagaaaagaa aaaagaaaat acattcaatt tttttactag ataatttaaa taccagttac
     7321 cagaaagttc aactattttt tcatgaaata attgatcaat ctttttggct tccaaccatt
     7381 tgccttcttt ccttgcttta ttataatttc ttacatactc tcttaatttt tttatatttt
     7441 cttcttttaa atttttaatc gcttccttta ttgcatattc ctcaagtatt attcttacat
     7501 catagacatt ctttatatcc tcctttgaaa attctcttat ttttattcca ctgtttctcc
     7561 ttacacttat taatccttca ttctcaagtg attttaaaac ttctctaaca ggagttctgc
     7621 ttattttaaa atatgatgca attttttcct ctgttatttt ttctttacca ttctccctga
     7681 agttggtgag cagatactct ataatctttt cttttaactc ttttgctttt gtcctcattt
     7741 cttaatttgt acacatattt tttaacttgt atacatattt tacctatatc tttaaaattt
     7801 gtcaagtttt cgaattgcct cattaaaaat ctaagcgaaa atgattcgtt gccttattaa
     7861 aaaaatctaa ttgaaattat acctcgcctc ctggaaaata agaagaaaaa ggaggtgaga
     7921 aaatgaggaa ggagttagaa atgaaagtaa ggaaagcgat aagcaagaca atatataata
     7981 acccaaagac accttatcaa agagttatgg aatgtgaaaa tatagacgaa aaaacgaaaa
     8041 agaaattgaa agaggtatat gaaagtttaa atccagttga gataaagaaa aaattgatag
     8101 aacttgagga taaattgttt gagatggtaa ggagaaaaag attgaaagaa aaaggagaaa
     8161 aaattgaaaa agaagaaatt ttcgcttaga attttatgtg aggcatcgta tataaatttt
     8221 acttagattc ttatatgagg caaaaggcta cggttctttc ttaaaggggt ttaaatccct
     8281 ttaaaatccc caaaaggtat caactcccct aaatgggtgg ctcaccatct cgccacccgt
     8341 gctaatcggt gctccttatt cgtcgcacca gcaccccctc aatctttgag ggtttaaggt
     8401 aattataact tgttctgggc aaaagacgtc agaaaaaaca cttggaattt ttcaaattcc
     8461 agtgttttat acctgtagtt gagcaagtat ttactctttt attatactct tctttaaaaa
     8521 attctgtaaa atccgataaa ttattgtata tctttttcaa ttattcaaga aagtttttta
     8581 ataatgtggg cggtacagga cttgaacctg tggcctcttc cttgtgaagg aagcgctcta
     8641 accactgagc taaccgccca gattttttat ttcctctggt ttaaaaatcc ctctttcagt
     8701 taggaaacct gttattaaat atgcaggtgt tacatcaaaa gcaggattga aaacattaga
     8761 atttaaagga catatatact tcccattaat ttttttaacc tcattttcat ttctttcttc
     8821 aattggtatt tcacttccat cttctatatt aaaatcaaaa gttgaaaaag gcgctgcaat
     8881 ataaaatgga atcttatggt aatttgccag aaccgcaagg gaatatgtac ctattttatt
     8941 tgctgtatca ccattttttg caattctatc cgctccaaca attacaatat ctattttacc
     9001 ctttttcatc aaatatccag ccatattatc acaaataaga gtataggaaa ttttattctt
     9061 ttcaagttcc catgctgtta atcttgctcc ctgaagaaca ggccttgttt catcaacata
     9121 tacatggatt tttttacctt tctgatgcgc cacaaaaaca ggagcaagag cagttccaaa
     9181 accacttgtt gcaagtcctc cagcattaca atgtgttaaa atattcatac cattttttat
     9241 aattttattt ccgtattcac caattttata acaggcattt aaatcttctt cataaatact
     9301 ttccacttct tcaagcaatc tttcaaacag ttcttcttta tcttttatac ttttatcatc
     9361 aactattctt tccattctat ttaaggcata aaaaagatta taggcagttg gtcttgaact
     9421 ttttaagaaa ttaatatctt tttttatttt ttcttttatt ttttcaaaag acaatttttt
     9481 attttcaagg aaggaaacaa tcactccata agcagaaaca catccaatta aaggtgctcc
     9541 tcttattttt aattctctta ttgctctgta aacttttttc acattatcaa gttttaaaac
     9601 aatctctttc cctggaagaa ttctctggtc aattatataa agtccgtttt tccaccagaa
     9661 tggttttacg ggtatttttt ccatttttat attccaggat gtaaacagtc ctctccaata
     9721 acttttttta attcttcaat aaattcttca ttcaaatcta ttccaaagtt tctcggttta
     9781 attttaatct ttttattatc atttgtaatt atgtttataa aaacaggaca gtcccctcta
     9841 aatcttaaaa ataaatcctt tactttttca aatttatcat ctcccagagg tattttaagg
     9901 tcaatttcaa ctttccttaa aaatttttct ttaacattat ttatagtggc aacatcatca
     9961 actataattt taatttcatc ctggtctttc ttcaattttc ctttaacaaa aataatatta
    10021 tttgctctta taaactgcga aaactctgca aaaacatttg gatagaaaag cccttttatt
    10081 tttccatcta tattttctat ttcacctatt gccatttttt ctgccttttt ctttgttgtt
    10141 gttcttttaa tatccgtaag aatgcctccc cagataactg tttctccatc tttaatcttt
    10201 tcaatctgtg aaaatggcat tgaataaagt tttatcagag caacgtattt ctcaatagga
    10261 tgtcctgtaa gatatacacc aagcatttct ttttcataag acagaagttt tgtttgtggc
    10321 cattctggta aggacttgat tgcttcacca tttaaagcag gtgttaactt ttttatttct
    10381 tttgatgtaa aaatttcaac gtgattttca tctttaagtt tctgcatttt atttccgtgt
    10441 tcaatcgcat catctatcat tgcaaaaagt tgtgaacgat aaattccaag ataatcaaat
    10501 gctcctgatt taatcaaact ttcaattact ttcttattta cactccttaa attaactctt
    10561 tcacagaaat cgtaaagaga aataaaatca ccttcctttc tgctttcaag aattgatttt
    10621 atagcagcag aaccgacatt tttaattgca gaaagtccaa atattatatc attaccgaaa
    10681 attttgaatt tttcatcgct ttcacatagg tcaggcggta aaatccatat attcattctc
    10741 tcgcattcct caatatattc tgctattttg tcctgattcc caatttcact gtttaaaagg
    10801 gctgtcataa attcaagagg ataatttgct tttaagtatg ctgtttgata tgaaattaaa
    10861 gcataaccag cactgtgtga tttattaaat ccatagccag caaaatttga tatattttca
    10921 aatatttttt ctgcaatttc tttttttaca ccgttggaaa ctgctccttt aacaaatttc
    10981 tccctcattt tctccatttc agagggaatt ttcttaccca tcgcttttct taaaacatct
    11041 gcttctccca ttgagaagtt tgcgagttta tttgctatct gcattacctg ttcctgatat
    11101 agtattactc cataagttgg acttaaaatt tcttcaagaa tctcatggtc atatttaatt
    11161 tttgatgggt ccttttttct ttcaatatat tctctgacca taccgctttt cattggaccc
    11221 ggtctgtata aagccaaaac cgctataata tcttcaaatt tctgcggctg aatttctctc
    11281 aacaaatttc gcatcccttc actttcaagt tggaatacac ctattgtttc tccttttgac
    11341 agtaattgat atgttttctc atcatcaaaa gggaattctt taatttcaat cccttttctc
    11401 tcttttatta aattcagtgt gtcttctata actgaaagtg ttttaagtcc gagtatatct
    11461 atttttaaaa gcccaatttt ctctatacat tcaaattcat attgagttgc aatttcacca
    11521 ttttgacctt taaataaggg aacatactca cttacatccc tttcagttat tacaatacct
    11581 gcaggatgta tggatgaatg ccttgcaagt ccttcaagtt ttactgaaat gtcaaataac
    11641 tgttttatct tttcatcact ttctttaagg gattttaatt ctggattttc attcagttcc
    11701 tgtttcagtg tttcatgttc atgggtaata agttttgaaa ttctatccgc ttctgtaaat
    11761 gtaaaattca aaaccctgcc aacatcttta acaaccgctc tcgcctgcat tgtaccataa
    11821 gttcctatct gacacacatt attctctcca tattttttct tgatataatc aataacttct
    11881 tcccttcttc tatcacagaa atcaatatct atatccggta aacttattct ctcgggattt
    11941 aaaaatcttt caaaaataag gttgtaagaa attggattta tttgagttat atcaagtaaa
    12001 taagcgatta aacttcctgc agcagaaccc cttcccggac caactcttat ccccttactt
    12061 tttgcataat ttacaaaatc atttattatt aaaaagtatc cggaaaatcc catctgtttt
    12121 ataattttaa actcataaga tactctttta agaatttcgt tatcactttt tccttcaaga
    12181 ttatctactt caattccaaa tttttccttt aaaccctttt taatcagttc ttcaagaaac
    12241 tcatcaaccg ttttatttga aggtggagta aattttggaa ggtgatattt cccaaaatca
    12301 aaggtcagat tacatttttc acttatttct aatgttgtat caattgcctc tggaatttct
    12361 ttaaatgttt ctatcatttc ttcaggtttt ttaaaatata tctggtctgt ttgaaatttg
    12421 aatctattcg ggtcatctat ttttgtcctt gtctgaatgc ataaaagaat ttcatgtgca
    12481 agtttatcat ttttataaat ataatgacaa tcatttgtag caacaagttt aatccctgtc
    12541 tttttactca attcaattaa aagtgttaca atttttttct cttcctccat atttaaaagc
    12601 ataacttcaa tataaaaatt atcttttcca aagatttcag aataaattcc cgcagtttca
    12661 attgctttct gaaaatcatc gtttcttaaa taccatgcta tctctccctg aagacatcct
    12721 gaaagtccta taagtccctt agaatattga gataaaacct ctttatctat ccttggttta
    12781 taataaatac cttcaagata agaaatggtt gagagtttca ttaaattttt ataaccatct
    12841 ttatcttttg caagtaaggt taaatgaaat gatgatctct gttcatctgt ttttctatca
    12901 aatcgcgact ttggagcaac atacatttca cttccaatta tgggtttaat accactctca
    12961 atacaacttg aataaaactt tataatacct gccattgttc catggtctgt aattgccaga
    13021 gaattcattc catatttatg agcgagagga acaacttctt caatcctaca cattccatca
    13081 agcaaactgt attcagtatg taaatgaaga tgtacaaact ttttcatttt tctctttgaa
    13141 gttttttgac tgactgatat attttacctt ctgttttaac actcggagca atagcgattt
    13201 caacaagttg catctccttt atattttttg ctccaagaga agccattgaa agtttaagag
    13261 cacccattaa attctgactt ccatcatcaa ctttggcagg tccaaataaa atttctttta
    13321 aagttccagt ggttccaact tttattcttg ttcctcttgg taaattctca tcacttgttg
    13381 ccatccccca gtgatatcct ttaccgggag attcaaccgc ttttacaaaa gcagaaccaa
    13441 tcataacagc atctgcaccg caggcaaatg ccttacatat atctccactt gttaccatcc
    13501 ccccatctgt aataatcgga acatatctac ctgtcttttt aaaataaaaa tcccttgcag
    13561 cagcacaatc agaagttgca gttacctgtg gaactccaat tccaagaacc tctcttgtcg
    13621 tacaggcagc tccaggacct attccaataa gtattccaga aactcctgta tccataagtt
    13681 caatggcaac ctcaaaagtt acacagttac cagcaatgat tggaaccttg gcttttttac
    13741 agaatgagta aaggtcgagt ggtttatatt ttgttgaaat atgttttaca gtcgttacag
    13801 ttgattgaac aacaaaaata tcaactccac tgctctgagc aatttcaaga tacctttctg
    13861 catattgagg aatacaactt actgcacata taactccact tttttttatt tcatgtattc
    13921 tttcctctat caaattttct ttaacaggtt ctttataaac attctgaata atttgagttg
    13981 attcctcagg agaagaggaa attatttttt caattactga gtaggggtca ttatatcttg
    14041 tatagattcc cattaaattt aaaactgcaa ttcctccaaa tttcccaaat aaaactgcaa
    14101 atttagggtc aacaacacca tccattgcag cagcaaaaac aggaatatca agtttgtaag
    14161 aacagatttg agtacttata tcaacctctt caggatttaa agtaacagtt ccaggaacaa
    14221 gagaaatatc atcaaaacca taacaaacgc gcgctttcct attaattcca atccacattg
    14281 ccattttctt tcctcctttt taaaagataa aattaaaaag taagtttcaa ttatttcttt
    14341 gacaagtttt tttctgacat gttttttcaa ctgactaatt tattatactc ctttttatat
    14401 tttaaaccaa atttttatcc ggtgaatttt ttatagattt tataatatta aaaagttgaa
    14461 agatttcttc aggagtgagt tgttcaaccc ttttctgtgg taaaatttca taatttgaaa
    14521 gttcttttac aagcacatct ttttcaattt taaaaactct tttaactaca tttgtaagtt
    14581 tttttcttct ataactgaaa attaaaggaa gaaattcata aaattttttt tcattttcta
    14641 tttttattct cggttttttt aatggcacaa ttttaacaac aactgattct actttgggtc
    14701 ttggataaaa aacattttta ttcaaaaagt agcatatttt tgtatcagta taaaagtaaa
    14761 gtaatacaga aattgggcag tactgtttac ttcctggttt accaacaaat ctttcaccta
    14821 cttctttctg gacggttaac agagcaagtt ttatttttct gtaaaattta agtatatgaa
    14881 aaactattgg agaacttaaa taatatgggg tatttcctat aactttaatc tttcctttaa
    14941 atgaattcca gaaattttcg tctgatttta aaaagtcatt tttaattaaa tggaaatttt
    15001 tatattcaga aaaattttct tcaaggaatt tacaaaagtc atcatctatt tcaaaaccat
    15061 aatattcctt tacattctca attatacctt cagtcaaaga accaagtcca ggaccaattt
    15121 caacaacagt atcccttttg ttcagatttg caaaagaaag taatttatcc cttgcatttc
    15181 tgtcaattaa aaagtgctgt ccaagatttt tttttatatc tatttttcct gtttctatca
    15241 gttttcttat ttcttttaaa attaaaaatt tcattttatt caaagcattt tataacaaat
    15301 ttatatgcac tgataaaact ttctggattt cctttattct tataagcaat atcaaaagca
    15361 gttccatgtc caggacttgt tcttaaaagt tttataccgg cagtaaaatt tataagtttt
    15421 tcaaaataaa atgtcttgag tggaataagt gcctggtcgt gataaattgc aattattaag
    15481 tcaaattttt ttctgaaaat tgtatctgct ggaaatggac cttctatatt tatcacttct
    15541 ttttttattt tttcaattac tggttttaaa atctcatttt cttctttacc aagaacacca
    15601 ttttcacctg catgaggatt taaaccacat actccaattt ttggattttt aattcttaat
    15661 ttttttaaaa attcatatcc aatttttatt ttttctgtta tatatctttc attcagatat
    15721 ttaaaaacat cctttaatgg aatatgggtt gtaagaagca ataccctgta attttcggca
    15781 atcattacca tggcatattt tctttcattt aatctttctg caagcagttc agtatgtcct
    15841 gaataattat atcctgcaag atgccatgct tttttggata taggaagcgt tacaatagca
    15901 tcaataattc ttttcttcca gagatttatc ccatctataa catacttaaa agatgcttta
    15961 ccacatattt tactgtcaat gccgactggg aaatttttgt tttttataat acctgtacta
    16021 taaacattaa aaaaatcttc ttttatgtct tccttttttc ttacttcatt tatattcaac
    16081 tctatcctta aattttcaat gtttttttca ataacaggga tatcgccaat tataagagga
    16141 ataaaatttt tcagattttt gattttttca tatcctttca acaatatttc aggacctata
    16201 cctgcagggt ctcctaatgt tataccgata attttcttca tcagagaaat tttacaggga
    16261 ttgttttctt aagttcttca ataaaatttc tgtataaaag tgaatacttt attttaaaaa
    16321 ttctatcctt tgcctccctg tatatttcaa gtttatccgc atctaaatgt gttttttctt
    16381 taaggtatat aacaatccat ttttctccaa ttttaaatgg aaaagaaatt ttttctttct
    16441 ccatttttga aatcttttct aaaatttctt ctataatttc tgaacttttg agttttgcta
    16501 actccatctc ttttaaactt tccgtattaa agtttctaac aaatttttct gcatcttctt
    16561 cattatcaaa ccatttgaaa aaaaactcat attcattgta ctcttcttca atttttttca
    16621 tctcatttgc aatttctaca ggagtgattt ctatttttga aataacttca tttcttataa
    16681 gttttcttat ttttaaagaa ttttcaattt cttcccgaag ttgtgaaatt tttatctgcc
    16741 tttcatttaa ataattataa aattctttca aagatgggaa atttttttta attctttcta
    16801 tttcattctt tatttcctca tcactaactt ctattttatt tttttctgcc tgataaagaa
    16861 gtaatttttc ttcaatcaaa acctgtaatg ctattggata atctatttta tctcttttac
    16921 ttctcaaaac tacttctttt tcaaatactg gcttatttcc cacaatagca acaattttat
    16981 cttctccaaa aacagttaaa acaaataaaa agaaaaatat taaaaatctt ttcatttaat
    17041 tttctccttt aaaatttcta ttttgtttgg atagattata actttacttt tttctttaag
    17101 ttttttcata aggttttcaa cttctacttt tttcttctgg gatataagta aattttttat
    17161 cataggagtt gcttctgtaa gtgttaaaat tcttttttct gttcttcctg tcaattttat
    17221 tatatgatag cccatttctg tctttataat atttgcaaga atctcacccg gtttcatctg
    17281 ggtaatcaaa gaagacactt ctggatttaa tttatccacc tcaatccagc caatttcacc
    17341 tcttttttct ctcgtgggtg ctattgattc tgtttctgcg attttttcaa atgattctcc
    17401 aagacgcaat ctattcagaa tatcatctgc tttctctttt gtatctacga caatttctga
    17461 aatctgaact ctttcaggaa ttacaaactc ctgtaaatga ctgttgtaat agttagaaat
    17521 ctcttcatca gaaatcttaa ttgtatctga aagaatatcc tcaacaaata cctgtgttaa
    17581 aacctccata taataatttt ttattttgct ttcaactttt tttctcaatt ttctttcata
    17641 ttttttagca tactgaaaaa gcaaaatctg attaacatag tcatctataa cacttttagg
    17701 gtcaacaaaa aaatattcct tataaatttc aggagaactt tcaatttttt caactatctc
    17761 atcaattgta actttatatc cattaacaac cgcaactgtt ttccttttat cttttattat
    17821 taaaaagaca attaaaccaa aaattaaaag acagaaaata acaagcccta taattccttt
    17881 ccactttttg aaaaacaact tcatttttat tactttaatt ttcatagttc cctcttatat
    17941 attatttttt tcttatttct atcataccac acaaccatct tttttactcc ggtaatttca
    18001 tcatcttcta ttttaccatt attattttta tcctgcactt tttgaccgag tgagataatt
    18061 ttaaacacat tactttttgt agtgatcaga ttaactattt ttgaaaaaac catttccttt
    18121 tcttttttta tatctataaa gaggtctaaa tcattatcct ttaaatcaaa accatatttt
    18181 gtaatttctt catttaattt tcccttttct cctttatctc cataaattcc aataacttct
    18241 gaaatatcct taaatggtct tgcatcaata atcctttctg caattttctc atcaacaagt
    18301 ggtaaacatt caagaacttc tttgcaggcg gtatttatat ttattcttcc acatacattt
    18361 tcatcaactt ttttataaca ggtaagatag tcaagaatat gagtatcttc tcccttccag
    18421 aaatttaatg ttttccagtt ttccatttta tgaatataag aaagttctcc aagggttgaa
    18481 aattgattat tcttaacata aaaacttgaa ggccaggcaa tagtccagtt ggctgcacca
    18541 aattctccac caatccatgg ttgaaaataa gtatttaatt taccaggact tggtggacct
    18601 aaaacccagt cgcttattcc cttcatcctc gggtcatttt tttgaactgt tacttctggt
    18661 ccatcaataa aatattcaca gaattcaatc aaatttccat ttttatcata gagttttatt
    18721 gtaaaatcaa gaccgtgttc tgcgaaaaat ggaattttag caagaaggtc tccaagaatt
    18781 ccaaatgaac ctggatttaa agcaactatc ctttcatatt gctgacagtt atcagggtct
    18841 ccatccattg gcaaaaaaag caaagcaggt ggagcttcat tcggaaatat aacaactatt
    18901 cttatattat ctccaattgt atagaaggag taaggcggga tttttttacc tgaaggaatg
    18961 gtgattgaag taaatctgtc aactaatttt gtaattttac ctgctttatc ctgatttata
    19021 tctgtttctt tattaacgaa atcatcataa acttcctcag atgtattaaa aacttcattc
    19081 cagttatttg aaaaaagaac aaaaacgcct tctattctcc atccacctat atcaatttct
    19141 tcaggatatg gattgaatat ttctataaac tgtcctcctt cttcttcaaa aaccctgcca
    19201 atcgcaagtt ctctgtattt ccattcttta acagcatcta tttcattcaa gtaagggact
    19261 ttttctacac cggtcatata tttatttcca gttttgtaaa cagacggtat tgaatcagta
    19321 tccctgaaat caattatatt taaagcaatc tgaattgcct gtttttcagg atatcccatc
    19381 ttcttaaaaa attcagataa ttcataaaaa cttgctttat ttatatcaat ccttttttct
    19441 tcttttctat tcaaatctct atcataggaa aaagttgtta taaaattttt tatcttttca
    19501 taattttttt ctccaagtcc gggaatcatt tttatttcag agggagaaaa gaaaggtcta
    19561 tcatctcctt taggttttat aaaataaaat tcatcatctt catcaatccc ttcatttggt
    19621 tcatcaataa aaccattgcc gtcattatct attccatcaa gaggattaaa tataatatca
    19681 ttatcgtcat caaaatttac tattcctggt tttccatcat ttccatacct gaaactaaca
    19741 atgttctgat aaattgattt tccaagaatt ttttcaagta tatttatttc aaaaacactg
    19801 tgtccttcat taaaacttgt atttaaattc cctgaagcat ttatatttat ttttccgctt
    19861 tcatcctcaa ctaaaaccgc ataccttcct ataatatttc cttttctatc tttaacataa
    19921 aaccattttg aatcattttc accatcatta tttaagtcaa cctcatctcc ttcaaaattt
    19981 gttctccagg catcaaaaaa acaatcatat tctctatcat ctttatcaat ttcccatatt
    20041 gcattttcaa tccctatttt tgaaaagttt tttgcctctc taccaaatat aatctttcct
    20101 gttgttttag cccagagatt aaaaagaaca agcatagttg aagtaaaaag aaatattaaa
    20161 agtaaaacta aaattacaag aattataaca tctccctttt taccatccaa ctttaattgt
    20221 ctttttaatc tccttttcaa tttctctccc ttctattttt ccctttgata caactatttt
    20281 aatttcaaca aattcaggca gtactggttc ttccattatt tctgtatccc atttatcctg
    20341 ccagtccttc ccatcaaaat atcttataga aaaatccttt acatcctctc caagaatctg
    20401 tgctcctgga aaaccatcaa aaaaactaaa atcaggatta tctttatcaa tccttaccat
    20461 atcaaccatt attttattat ctttccagta aataccaaat tttgctatat ctccttcttt
    20521 atcctctgaa aaaggcgaaa caaattttac ccatttatct ccacccttaa aattcattct
    20581 gtattttccg gaaaggtcat ttatcattgc atttgaaatt ctactttcta taaaatcaga
    20641 aataaaaaca atttctttta aaatatcctt ttcaaccgcc ttttttcttg aagataaaag
    20701 cgaattttta agaataaaaa tagcagtaaa acagacaaaa gtaaaaacaa aaattgctat
    20761 caaaacttct ataagagtga accccctatc ttcttctgag agaggtaaca aagtattttc
    20821 tctcatcctt accttctttt ttccataaaa tttcaagttc aactttataa aggtcttctt
    20881 tctcttcatt aaaattcact ctgtatttat aatcaggata ttcatttaaa acaccttcaa
    20941 tattatcggg taaaggcaca tctttatttc cgtaaatctg ttcaatctga gtaaaaatct
    21001 tatctgcaat aaaagaaata ttaaccgaac ttttactcct ttcacttaaa ttcataatta
    21061 caggataaac ttttataaga gttataattc cacaggcaag aataaaaagc ccgattaaaa
    21121 ctgttatcaa agtaaaccca tatttcaatc ttctattaca catttccctg ttatattatg
    21181 tatctttatc tttttttcct tttttgaaac ttcttcaagt aaaattatat gtcctgcctg
    21241 ttttgctgtt ccatcaggga gaaaaacaag aggattcaca ccatctgttt tttcttttat
    21301 tctgatattt tctggcatct ttatctcttt ccatacaggt gattttcctt cttttaaaat
    21361 tctgaaactt ctctcattaa aaattacata aaaactgcat ctctcattta tagcatattc
    21421 tcttgcaaaa ttcacaccct ctacaatttc agcacaggca gaatttaaaa gatactgctg
    21481 atgatacttc tgaataaaaa ttgtaacagg aattaaaaat aaaagaaaaa taaaaagaac
    21541 aattaaaatc tcaagaaatg taataccttt tttacccata aagttattta ttttcaataa
    21601 attctttatg aagttgagtc agtgctttct gtccatcctt ttttcttatt acgcaggata
    21661 tttttatttc agaagtactt atcatttcta tatttattcc tgattttgca agacagttaa
    21721 acatttttcc tgcgacaccc ggctggttca tcatccctat acctataatt gaaaccttac
    21781 aaatatctga atcagtaata actttttcag cacctatctc tttaccaatt ttttcaacaa
    21841 tattttttgc tttttccata tcatttatgt ttacagtaaa agaaatatct gcagaattat
    21901 cccttccaac attctgaaca ataacatcaa catttattcc tgcctctcca agtgctgaaa
    21961 atatttttcc tgctattccc ggtttatcag gaactttaaa aattgaaatt tttgcctgat
    22021 tttcatctat tgaaacagaa gaaacaactg caccctcttt taattccatt tcctttacca
    22081 ttgtaccctc cgtattttta aatgtggatt tgacataaaa aggtacttta tatttttttg
    22141 caaattcaac cgctcttgac tgcataactt ttgctccact gcttgccatt tcaagtaact
    22201 catcatagga aataacagat atcaattttg cattttcaac tctattaggg tcagcagtat
    22261 aaacaccttc aacatctgtg taaatttcac atatatccgc ctttattgct ccacttattg
    22321 ctattgcagt aaggtcagac cctccccttc caagagttgt aattgaattt tctgaactta
    22381 tgccctgaaa accagccact acaacaattt tatctttatt aagttcttca ataatttttg
    22441 atggttctat tttctgaatt cttgcttttg tatgtaatgt atctgtttta attcctgcaa
    22501 aatatcctgg aaaaccttct gcctgttctc cataactttc aatagccata cataaaagag
    22561 caacagatac aatttctcct gtggataaaa gcaaatcaag ttctttttca ggtggatttg
    22621 gcataatgtg ttttgcaagt gatattaaat tatctgttgt ttttcccatg gcagaaacaa
    22681 caaccactac tttattaccc tttctctttg tctcaaccac tttttttgca acaaatttaa
    22741 ttttttcaat atctgccact gaactcccac catatttttg aacaattaaa gccatcttta
    22801 ctcctttttc taataaatta taacatttag tttacaaaaa caaaaatcat gggtataata
    22861 aatttatgga gagaaataaa gaacttgaaa aatacaaaat aatctggaat aacattcctt
    22921 gtattatttt tatacttgac gaaaaaagaa atatccttga agcaaatgag aaaataaagg
    22981 aacttgatta tgagattgaa gatttaattg gaaaaaatct ttatgacatt ccctctttta
    23041 tttcaaaaga ttctctaaat aaattatttg aaaaaaaagg ggaaattata aaacttgatg
    23101 tcttttctgg aaaaaaagag aaaaaaatag ttaatgcaca gttaatagaa ttagaagaag
    23161 aaacagcaag gtatttaatc ataatccatg atataacaga ggaagaacag ataaaaacaa
    23221 aattatatga agacgaagaa ttgcttaaag agtggtttaa tcttgctgaa aaaagtgttg
    23281 ctggaattta tatttatgat gaggatttca attttttata tgttaatcct tccttctgta
    23341 atattacggg ttacactgaa gaagaaataa tagggaaaaa gaaatcatat gaacttgtgc
    23401 atccagatga tgttaattta tcaaaagaaa tggcagaaaa gagatttaaa ggagaaattg
    23461 aaatagcaag ttttaatata agatttaaaa gaccagatgg aaagataaga caatgttttg
    23521 caattggaag agttggaaga tacaagggga aaaaagttat aaccggaact ttaattgata
    23581 ttacagatag ggtaaatctt gaaaacaaat taaaagaaga acatgcatta cttgaaaaaa
    23641 cattagatgg aacaattcat gcaatatcaa aaatagttga aattaaagac ccttatacag
    23701 caggtcatca aataaatgtt gcaaaattat ctgaagaaat atcaaaagaa attggattta
    23761 ataaagaaaa aattaaagaa attatatatg catcattact gcacgacatc ggtaaaattt
    23821 ctgttccaac tgaaatcctt gtccttccaa gaaaattaac accaattgaa ttttctataa
    23881 taaagacaca tcctattgtt gcttataata tactgaaagt aataccgaat tttgaaaatc
    23941 ttgctgaaat agttttacag caccatgaac gacttgatgg aactggttat ccaagaggaa
    24001 ttaaaggaga aaaaatttta aaagaagcca gaataattgc tgtaagtgat gttgttgaag
    24061 caatgattaa tcatagacct tatagacctg ctttaagttt agaagaagca cttgaagaaa
    24121 tagacaataa taaaggtata aagtatgatg aagaaattgt agatgtagtt atttacttat
    24181 ttaaaaagaa aaattttagt tttatttaga caggtataat ttcaaactta aaatcatttc
    24241 cacttttcat aatacttaaa acttcaaatc taaatggaag attaatcttt tttgaattca
    24301 gataaatttg ggcaacctgt tcaatctttt tcaattttct ttcattaact gcttcctgag
    24361 gtagtccaaa attatcagaa cttcttgttt taacttctat aaaaataatt tcttttcttc
    24421 tctttgcaat taagtcaatt tctcctattt ttgtcctgta atttttctca attattctgt
    24481 atcctttctt ccttaaaaac tcaactgctt tctcctctcc aagtttcccg aactcaatat
    24541 tcctcattta tttttatttc ttaagtaata catttttgct cttggtgcaa gacgatttct
    24601 ttctcttgtt tttaaaactt caatctttac aatattagga gaatataatg gaaaaattct
    24661 ttcaactcct tctccataag aaatttttct tactgtaaaa gattccctta ttcccttccc
    24721 ttttcttcca atacacgttc ctgtaaaaat ctgaattctt tctttttctc cttcttttat
    24781 ttttatatga accgctattt cgtctccagg ccagaattca ggaatttctt tacttggttt
    24841 caatttctct tcaatttctt caactttttt caatgtcatc ttttcctccc tgttttatat
    24901 atttttcata aaggtcaggt cttttttcct ttgttctttc aattgccatt tttcttctcc
    24961 atctttctat ttctttatgg tttccagaaa ttaaaacttc aggtactttc atccctctaa
    25021 aattttcagg tcttgtatag catggccagt caaaaatata attgtaaaaa ctatcataac
    25081 ggggtgcatc ttctggtaaa actcctttta aaagacgaac aacactatca actattacca
    25141 tcgcaggaag ttctccacct gtaagaatat aatcacctat tgaaatttca taatcacaga
    25201 ttttacttac cctttcatca actccttcat aatgtccaca gattataaca agtccctttt
    25261 cttcactcaa tttttttgca atctgttgat tataaagaat acctgttgga cttgttaaaa
    25321 taacctttcc ctctctattt tcttctttaa ttttttcaac cgcatcataa attatatcac
    25381 attttaatac catccctttt cctccaccat atgctttatc atccactttt ttatgtttat
    25441 cctttgaaaa atctctcaga ttccatattt ttatctctac aatttttccc ttttttgccc
    25501 tttttacaat acttacatct aaaggagtaa atatttcagg gaaaatggtg attatgtcaa
    25561 ttcgcattta agtttatata attccttttt tctttaaaaa tgattcaact gtcttactcg
    25621 gttttgctcc tgtgttcaac cagtatttaa ccctgtcttc ttttacctca aaaattacag
    25681 ggtcaggtag agggtcatag tatcctaaaa tttcaagaac atctcctctt ccatcttttt
    25741 catcacttac agccacaact ctaaaaaaag gtctattcct ctttcctttt ctcaccaatc
    25801 ttatttttgt tgccatattt ttttctcctt ttatactttt tcctctttct gtgtctctat
    25861 gcttttatga actgcaagag caatttttgt tacttctaaa ccatcttctc caaaagcagt
    25921 tattgaattt tcccttttca aatcttcaat cttataccca ttttttatgt gaattatatt
    25981 ataaacgaaa tccgcaatac tttcaatacc atatccagaa taaaaggtat gccccatttt
    26041 tttcacctca ttaataaaaa caggattata ggtcataact ccattttcct gataacaaac
    26101 tcttgtaccc ctatcctgac tgtcgcattc tataactcct ttttctccaa caagtttaaa
    26161 tccttgatta acaatactct caaaattttc aggtaaaatc catgaaagtt caaaagatat
    26221 aactgcacca ttttcaaatt caacagttgc aacaactgca tcaaaagtat ctattccaag
    26281 tgattttaat ttatttttta ttcccttggc ataaacattt ttaccattac ttttcaaaaa
    26341 ccatctcatt aaatcaaaaa aatgtacacc aagaaaccag acaggagaac ttttaggagc
    26401 ccagttagga aaccagtccc ttggcacaac aattttatcc tccatatgaa cagaaccgta
    26461 ttcaactttt ccaatctttc cctcatctat atccttctta attgtctgat gaaatgggtc
    26521 aaacctttta tgaaaatcaa cctgaagtaa aacattattt ttcctgcatt cctctatcat
    26581 ttcattacat ccctcaacag tcacatcaag tggcttttca acaaatacat gaattttttt
    26641 atttgcagca tataaagttg gttctttatg aaaagggtca ggagtagcaa ttgcaacacc
    26701 atcaagttct tctttctcaa acatttctct gtaatctgga taacccttta cattaaattt
    26761 ttttacctga ctttcaagaa ccttttcatt tatatcacaa attgcattaa gtttacatat
    26821 tccagatttt tcaagttgac tgtatgtatt taatatattt gtcccgaatt ttccaagacc
    26881 tattattcca agttttatca tttttccacc tccattattc ttaaaactgt tctaattaaa
    26941 atttcacttg ctttcttaat atcatctatt tttattttct catggtctga atgggcatca
    27001 tcaagtttcc cgggaccaaa aacaacaaca ggcattttcc ctactctgtt aaacaatctt
    27061 gcatcacaac ttgcaataaa accttcaaca acaggtacaa ttcctgtctc ttcagatgct
    27121 ctttccatgg ttttaacaag gatatgggaa ggagaaatgg aataagaatc attatgcaat
    27181 tttggaaaag aaagtttata attggaaaca atccagtcat ccccacagtt tttaataaca
    27241 tcttcaaatt cacttttta
//
LOCUS       DAOSAT010000013        25675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_1383_length_25675_cov_6.100898, whole genome shotgun sequence.
ACCESSION   DAOSAT010000013 DAOSAT010000000
VERSION     DAOSAT010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25675
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1383_length_25675_cov_6.100898"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLF90_02050"
     CDS             <1..263
                     /locus_tag="PLF90_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56100.1"
                     /translation="WRKTKEFPSGYIIEGHAIIQKWGEGGSYGICGSRSCMRSCFNYL
                     EKNNIIEQTFKGGEFIKRERWLLPYRVKKENKDEKIKGKRQI"
     gene            232..1386
                     /locus_tag="PLF90_02055"
     CDS             232..1386
                     /locus_tag="PLF90_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK56101.1"
                     /translation="MKKLKEKGKYDVVVVGGGIAGVSASVSAARNGVKVCLIEKSCCL
                     GGLATLGLVNVYIPFCDGKGNQIIKGIAEELIRVCGGDGNAKIPDYWLSENKKEQRKT
                     TRYFAEVNPFYSIIALEEFVLKEGIEIKYDTIVYDVIKKEDRITDIIVLDKEGTGKIS
                     CSTVIDATGDADICYFAGEETVSVDKNVPCGWFFYYDGKNLKKYTFTEHFDRYNQDIP
                     EGIKTFSGVKASDITEQVLESRRKIKELFFKIKEGKTDAYLLSIPVIPTFRMTRRLKG
                     KFELREEDEGKIFPDAVGITGDWRKPGPLYYIPFSCLYGVKTKNLITAGRCISASTAW
                     DIIRVIPTCALTGEISGTASAILVKEKIESFSSIEIKKLQKVLKKQGVIF"
     gene            1507..3921
                     /locus_tag="PLF90_02060"
     CDS             1507..3921
                     /locus_tag="PLF90_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOK56102.1"
                     /translation="MKMIFLGILIFFLNVIKGFSYSEIDIETFNYRNISEVREKWLPQ
                     ERSREVELIKEKGKKVIKFVLNFSEVEKRSYWDRFVLLDLSNFEKFVIEVKSENPSLT
                     TNCTIYFESEGGWFSYRFPIENNSWQKIIIPKSKFVIEGNPTGWDKIKRIRISFWKID
                     TTDLKETSVYLRSLKGIIPDSKIGIVFCDSAIKEKGHRRDVISKYYSDIAGIFDKFEI
                     DYKTVNQSEFEKKEIEKEFKILIFPYNPFFSEEEIKNIIKFVEKGGKIIVFYSSNQEI
                     YKILGIENAVYRYESYPGEFDTVEFLPEIINGLPEKMKQSSWNVMVPEKISENSKIAG
                     YWIDADGKTTGIPSAILSENGFYFSHVLLNKEEGEQFLISLILFFFPENKNAIFDSLI
                     KNTGKLSGFQNLEEIKKFIEGKMIKLSFYKKREVKNYLNITISKYKELTKNYKKLNIE
                     DLFKKKNEIDENLRQAYFRCFKGKEKEYRGVWCHSPFGVKGLAWEESIKKLKEANFNA
                     IFPNMLRAGIAYYKSEFLPVAEEVKIKGDQIEECLSACKKYGIECHIWKVNWNLFNAP
                     AEFIEKLRNEKRLQIDKNGKEVLWLCPSNPLNFKLEFDSMLEIVKKYDIDGIHFDYIR
                     YPDANSCFCENCKKNFEREYNVKIENWPYDVINGKYMRLYKKWRQEQITKLVREVSNE
                     ARKIKPNIKISAAVFSNYPDCKETVAQDWKLWIEEGYLDFICPMDYTISNYTFKNYVK
                     NQNEVIRKKISFYPGIGAYILSPEDIVNQIEICREIGTDGFILFDYNFTLYKNLYYFG
                     LGITEK"
     gene            3963..4775
                     /locus_tag="PLF90_02065"
     CDS             3963..4775
                     /locus_tag="PLF90_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMO1 family protein"
                     /protein_id="HOK56103.1"
                     /translation="MEKIRDPVVSGYFYPSGKAHLLRMLETFIDRNTQKEKVIACMVP
                     HAGYIYSGKTAGLTYSKIEIPESVIILGPNHTGYGEPYSVSDHDKWLTPLGEIEIDKE
                     LRDKLVEKSRYLQKDEIAHIREHSIEVQIPFLQFLKKDVKIVPITLMGYVDNPAWYEI
                     GEVIAEVIKESKKECLLIASSDMTHYEPHKIAESKDNYVIEAICDLDEDEVIRRIEEK
                     DVTMCGYSGVIVSIVASKKLGAEKGQLVYYTTSAETSKDYSQVVGYAGVIIK"
     gene            4778..6679
                     /locus_tag="PLF90_02070"
     CDS             4778..6679
                     /locus_tag="PLF90_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56104.1"
                     /translation="MFFSNLFFLILNSFLLAGIFGVSGIIEFFVIFLIFFYFQIYGIS
                     IILGLFKILYPQFILIFLFLIFLFLLPEIKKIKLPDLKNIPFKISIFLCLTVSLHLLY
                     IFNLLILPPLTTDGLLYHLPFAVHYYKTHSISLPNLYFTEISMTYYPIGGEIFYFFSI
                     LSKKEFLFKFTQFPFLILGCFSVFLISKSFGLSDFLSTLSAISFSLIKPVFKESTMCF
                     VDLMMASTFITTYYFFNKKEKKYIFPGLLSLSLLLSIKTLSLLFALLLLPFLFQKKSG
                     KFSKLLYFSVFYLLFFGLFSYWRNLLLTGNPVYPADITLGNFTLFRGAYIYQKVSIIE
                     KIKNLFSVLAFSSLHIDPSFNLKIVLLLFFLISFTLSIRKREILIFYFLFPLSILLYF
                     LLIPSTYFQIRHLLPVYGILSISLFYPFQKLEYLCIAVFLYFFLSVFPSSCISLFLII
                     FSILFISFFIINRIKKPVFYVPLLLLFILFLIFQVDIKTPLYKEIKFEFWKSFYKEEG
                     EIWEFVQKNSKEGKNIAYVGSFFIYPFYGENFQNNVFYQSVNSIKTIPVYKYRKRIKY
                     EEPSENLYRENPDFKKWIEGLKNEKTDWIIIKKDKEYIERKWIEENPDIFKILYSTNY
                     AEIYKLKIE"
     gene            6781..7869
                     /locus_tag="PLF90_02075"
     CDS             6781..7869
                     /locus_tag="PLF90_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK56105.1"
                     /translation="MKNGMPDYLIIEKELREKIEKGEYKSGERIPTEVQLCEKYKVSR
                     ITLRKALSILEAEGYIYRITGKGTFVIPSEKRDEAKIIKERQKEIKKLNKGVAILVPC
                     ITFTIFSGIVRGAEDYLREENYHLLLGNYDNNPEKEREYMEMFTERGIEGFIVSANYF
                     SNQNSYYEELIKKNIPFVFADTPLEKFKADLIATNNIKGAYLGTKYLISRGCKKIAFL
                     SALMILYSSERRFMGFRKAMEEGGLKPDEKIIKHGYATEEFGYDSIKEIMKKEKIDGI
                     FSANEPITVGIVKALKDMNISKDDIKIVSFDQPVLPNGTDYDVKLIKQPRYEIGKISA
                     KILLERIKEKKKGINSIYKEILLEPIIE"
     gene            7883..8779
                     /locus_tag="PLF90_02080"
     CDS             7883..8779
                     /locus_tag="PLF90_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOK56106.1"
                     /translation="MKKKMGFTLIELLVVVAIISILAALILPALEKAKEKAQRAVCMS
                     NLKQLYIALSLYANDYDGYFPIALENQYNRIRTSRSMMLLLGIYVSGPAGTSPPSGPP
                     TGPRYVTNPELFICPASRDKVSKTGYLVYPYCSYAYAPNLNTKKLKVPAGAIEIGTGK
                     TDTLSSNNWAIMSDKKYVDDNGSFEWYRGFGSYWEYRFQLVELCNHKTYGINVLYING
                     DVQWIPAFQWLGPTGWTKSVSQGGNYYINGKWYIPSEKIPNINYSMGGSQPYWYTSNT
                     GSSYVHPDQQIYLRGPESTGNF"
     gene            8791..9663
                     /locus_tag="PLF90_02085"
     CDS             8791..9663
                     /locus_tag="PLF90_02085"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOK56107.1"
                     /translation="MDKRKFSGFTLIELLVVVAIIAILAAMLLPTLSKARERARRAIC
                     LNNLKQIGLAFRMYAEDYSGWLPNYEGPPTSSWYVLTYRPFNKLIGKDSSGNLGGVKY
                     VTTAEVFLCPSQRLDYRNVSSVRKTGYLTPQYQLSYAYAKPYDISTWARVSYSINPYA
                     YVPGLGINAGTEPDSILAADRQRPDATKGTGNLGGGPLWVIDRWDWGGATGTEDQPVA
                     EGSIPKGITLTEENNHGTAGINVLYMDGTVRWIAATKTEVSGEIKYILPMKRNYEGIT
                     QWVGRMHNPLIYAP"
     gene            9680..10339
                     /locus_tag="PLF90_02090"
     CDS             9680..10339
                     /locus_tag="PLF90_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOK56108.1"
                     /translation="MKRNGFTLVELLVVIAIMAILATLILPQLAKARIRAQQAACISN
                     LKQLGLAVRMYANDYDDCIPYMNGDDSGRAYNNHLSLLLPYVGWKQGTTVTPELYVCP
                     AVGLSGAPDATYSCFPFTNVKIKRIYCFNRITGGFPSGYGNRVYNGQKLGHVPDPTTH
                     ILFLEGTCDHVYSLSQIMYLHGNNTNVLFAGGNAGSFPKGFLENPANLVPYFGQKLAG
                     Y"
     gene            10364..14413
                     /locus_tag="PLF90_02095"
     CDS             10364..14413
                     /locus_tag="PLF90_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56109.1"
                     /translation="MKKIFLSTIIEFFLFSSFLLFSDTNLWEGKSSDWFDKENWSMKK
                     VPDKNDNNDVLIPSGKLNYPKLTSDVFIDGTLTIEKGASIQLNGYNITLVNLGVNNKK
                     GIVNKGIINAEKNGSILTVGRGGFINEGEVKGYPSLVFGTYSFDALLKAGGANFSSIT
                     ISPGSYSYRVTAVDKLVIKGNLVLQGHSLYVNKNASVYIYGDIIFEGKSPRTAGMTLE
                     GDVFLYGNIVSNDSSIGNGNGNGWIIFNKDGLQEINILKEGGILPPIKIEKKEGIVKL
                     TGNLSCAGLYIEEKNALLLNNTEKIQFGGAGSESAYTRGLINKGKIQGKTDLNFSTGC
                     YNADLLQGDFEIQNLKIDTGNYAYSVNLKENLKVNGDIDFKTGTLNMGENAITVGGSW
                     YFSPFPEKPSPVTPVLQSGKNAKIIFTSNSNAIIQSGGVEKAHYYDATFPNLIINKPE
                     GILIIKEKPLEVKGMLKLENGKIEMKDGGEIYLGVVRDQTYGKYRIFDIGLKLKGTLT
                     KEMFPEIVPAKIPEPTPGKVRIIHNYVGPDISSKELFNIAPFANITTIPYTTIVKNLI
                     DNDPDLRTLTLPETGTPAKFSRYQFQFKEKQKISRISWCVASGPWAILADKDGDGNCE
                     TLLRIDIEGKVENPGGTWVSRSFLHNNFVPPVETYGIMYVDFSGKNNIYDFQILISDK
                     KDYDKLKKDFEYEKEKGVPIVEDGEEINVPAPSEKERIFTGFHIEPWMFNLPQWIKMD
                     KDKRPSLNEYKGFRDFVEGVKNYKGNILNMWPPTTWEQVGPGTYEHPVLWPSEYDRHS
                     AKENFLEEVVEAFHKEGLKLFTMRRVAYPKKLEEFPKTPTRDKPAPYISRHSREYLNG
                     IVREQVLSGVDGVGIGFDEQMPYRTILNLKGVNEVTKSIFKEKYKCELPEEPQDTEAF
                     RKWIVFGYEEFANYLADAANEAKKIRPSVYTKSPVHFTLGTLWNSRIDVGIAEDIVGN
                     KARIDFARGNSYLTFQSLGHYVTVLSAKIGVGANENGGVVSLHNCSWSWRDDTLNVHP
                     GFYLHFPPVYMRASPISSVLNGARMTLFWRYNLIFFGNYDKYVKEGYSIIETMSSWGA
                     KEAKTPKYIAILRSRTSEDWWQVKQRYNEKGDPMDQIRGYIYLKYVMEFLLRNGYPFD
                     VYYLDHPVENLRKKLDGYKLFILPFPYSISKETFNLIKEFMEKGVGVILFDRTGETDE
                     WGNLYEKGLFEDLIKEKKVLFINEDIPKIGHHPDFIKSLGQKIDETLGREKTFYFNSY
                     GEDVEFGILENKNKEYILTFINWTDKPVEVDFGLLKLPQTKNYEILQRDLNGTKRVKI
                     GNKELLKGMDLKKIRIALDKWDVKVLYVK"
     gene            14427..17051
                     /locus_tag="PLF90_02100"
     CDS             14427..17051
                     /locus_tag="PLF90_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56110.1"
                     /translation="MKKIIEFACVLLILTCFSLKAERDYKLDWYNWVEVKTAPYMLKI
                     DNLTDSDTKTVSEYLGGPEIGGWIEWQFPSPVNVSYVKFMQSGADTFSLMCDTDGKLL
                     FNIEVGKVKVEKPDEWIIIPVNKKVFGLKLIALSGKAGYRTPYPTFKEIEIYTKEKIT
                     PEKKREEKSKAILNKGNKIQFPELNKKNIKFIPCIDLWMAGIIPGDKMQESTEEIEKS
                     NGFQTMLKKLREIDAQGVRIFLETYCCNNKMPWKSQLAPNYGKDTLKGFIDALHKNNL
                     TSAIFLHAWISPIQPAEKMLPMPDRRWDYPYEQSDLVASKGLYEGYEPNRYPCVICDD
                     DFKTKWFGLLKEVVERGIDEVYVMPDEYYFKGHNLETVRCPSCEREFKKRYGYESLPK
                     VGKGGGIKRDFGPDPRKMIDNEHYRKWKLFEYEKIAELFNEVADKLKKLNPNLNLISS
                     ANPASSLYSNLGLEHGIAFDIIGRGKSFTHIQLYAHVPLNYGGYTALARRMSAAYPDA
                     NLSASIQSLGFKHLKADKSNEILFYGYLIPFIMNGATRIDLYRLNYVEGNGWWERIKN
                     GIKMVRLLEEWGITESKSVAETCLLISRASEDWWQVKAQSAMGTVEPESKRSTILYME
                     EKLGTVQTETTDEMERTLNFERFRGMYSNRAIESVLIENGIQYDVKYTEREETLGDLS
                     KYKLLILPFSYSMSKKAYENIKKAVESGTKLVIFNQLAPTNEYGEPYSEPLLKELLNN
                     PNVKFIDINPASEGMKRSFKKFYGDLFYKLLGTKYYFNNNDNKVEYVLRKIDNKNFIL
                     YLANWDKNESTCVIGIPVASGNYRILIYSSLTDELREGTMKGTFGNKNIIKGYSFKNF
                     GIKISPGEVLLVKIYPEK"
     gene            17048..18925
                     /locus_tag="PLF90_02105"
     CDS             17048..18925
                     /locus_tag="PLF90_02105"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOK56111.1"
                     /translation="MKEEKIVLKNDILKIEIDKKDFYLSVFDLRTGIKWLMQKNGPGD
                     IGIKGHSGPWIGLPFSSAGSVEWKENTNFIEVWLTRFPYRANIWGPIDFAVFLIFKLM
                     EDTLSIIVGTKNGRGEVSLIDSYYPRGFLFPEKVNGFLVLPYGHGCLLDKKFPCELDH
                     TFPVYAGMSFVMPWWGQITEKGTGLIALPETPEDMGIRLITENGYGHTAHPYWQASLG
                     NFSYPRKINYKFFKKCDVVLLAKTYRKFAEKRGIKGILKEKAKERKNVEKLKGSIIVS
                     IWHESWFGNFRKKDVWRRMTFAEGLKRFKMLAKDVPIKKAVVHIDGWGIKGYDCCHPD
                     ILPPDPKLGGWNGLKKMADEIQSMGHIFLLHDNYVDVYADSSAFGPEYTVLDLSGVRP
                     ENNEWLGGRQRWLCPKKTMKFAKRNLMEVKKKINPSGTYLDCFTYSHLRECYDQKHLS
                     SRKDTIKYWSEIFALCQNFGWVTSSEGGADWSIPVLDFCHTVQPGICPFDLREKLKGP
                     LGIPVPLYSLVWHDCIVIPAWISGEKDTDSFLWGILWGGIPSIRTKSLECNSAPYGKE
                     DIKFVRNLIPMVELSEKICFEEMVNFKILDSNGNIQQTEFSDGTSITVDFKNKTYWIK
                     K"
     gene            18922..21234
                     /locus_tag="PLF90_02110"
     CDS             18922..21234
                     /locus_tag="PLF90_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK56112.1"
                     /translation="MTKYQLTRKVKKFVLDNGADLVGIAPVSRWEKAPIKHSPKGIMP
                     ESKSVIVAGVHFLDANTELAAEKDPRFPGPGYSEMNASVFLQSLGFKIAKFLQDMGFS
                     TIPIIHTMLWNYRPQEGAERGWMADMCHYYAAVCAGLGEIGWNNLCLTPQFGPRQRFI
                     SIITEAELEPDPLYNGEPLCDKCLLCAKNCPTESFDKEVIRIATIEIEDKKFSFPYRN
                     LWRCAIGENFQLDVFIPKPEKIDENFILETIDRGIKEKPEVFYGWKMGMCLKYCVPPK
                     RRYFDRNYCKSPRRKRDKEPDFSEENVKKLIKEIKKLALKLDVDIIGAASIEEFKESG
                     INLNQFLPDAKSALILGFSYPENCSLNTWFTAEVAELLIAKHIQEKYGFSSLIKSGID
                     DEKCMKICNLTYYSKGITFSEKNKILRTIITQAPFNKNSFKSTKKREKKKTLSTSSLT
                     EYIKTFSLKNGADLVGITCVEKLDEIAEQLKNIYENEKYFIVENKGWVCDGKIIKDLM
                     HAPFNPVVKEVKLIPRKPSDYLKSARSIIVIGLHLIEKSIELAGSPPGYKAGHYMYTT
                     HMESINQIFTILKKITRELSSFGYQCIPVIDIFDISSKIYGEIPDLYSSRFAAIASGL
                     GEIGWNGIVLTPQFGPRQRFACIITDAELIPDSVYKGPSLCKKCYECVNACPVSAISE
                     KDKNFIKIDGKVFEWGKLNILRCDCAKRYALIADEGPKYIGSKNNFKLPEKITPEFVC
                     EALKKSDRIQEPGYCTIVENCFKICNGRKK"
     gene            21235..22257
                     /locus_tag="PLF90_02115"
     CDS             21235..22257
                     /locus_tag="PLF90_02115"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOK56113.1"
                     /translation="MESKAKEFLKRLVETPSPSGFERIVQNLVKDYLKNFIDRIEIDI
                     HGNLIAIKNPSGIPKIMLGAHCDEVGFQVKYISEEGFIFFQTIGAPYIHSLHGQKVII
                     LNKKRCVKGVICKKKIDKAEDLKINSFWIDIGAKNKEEAEKFVSIGDPIAFSSSFTEL
                     LEGKNIMAKGFDDKIGVFVIAETMRLLSKEKIKSSVYGVSTVQEEIGSRGAWMASFKI
                     KPDIAIVVEVMECSDYPDTEKRIMGDIRLGKGPVLQIGPNITPTLGEFLIETAKKEKI
                     PYQIDAAAGPTPTDASVIQIIQGGIATALLRIPLRYMHTSGEIVSQDDVKNAIKLLTL
                     SIKNMF"
     gene            complement(22270..23148)
                     /locus_tag="PLF90_02120"
     CDS             complement(22270..23148)
                     /locus_tag="PLF90_02120"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOK56114.1"
                     /translation="MIKIALLGYSGSGKTYIFKELTGKESEIFDPFKPNIGIGIYKDE
                     RLEKIKEIVKPKKVVYPEFEIYDFKGFPEGEGFPSNYFKNFFEADIILLIVKNFAEEA
                     NPEEEMESLVMELIYHDIERVESIIEGRKKEKTNTEIWEKCLDFLKSEKFLKDMEIDK
                     KQLLGAELITLKEFLILSNGNRKEIKTDLPVIYEIKDIYKKVIEKLGMITFYTIKGDI
                     AQAWIIPENLNAKQAGGKIHKDIEKGFVKAVSLSFNEFVEITDWHKAKNMGILKLLGP
                     NSHIEDGDIIEFYFTK"
     gene            complement(23145..24242)
                     /locus_tag="PLF90_02125"
     CDS             complement(23145..24242)
                     /locus_tag="PLF90_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56115.1"
                     /translation="MTKRELNIKIFEGKANKVLWQPRLETWIYYHRKNNSLPDSFKDL
                     SDFQIYDQLGCSVRYGASSGLFEYNEEPVEFFERILDENHTESVVRTKWGDLRTVYQI
                     VRENENKRIVKFPCENIKELKILTHLIRNKRYIFVKEIFDERDKRMGNRGAPTIFLSS
                     SGFTDLIKFYAGLLNTYYLLKDYPQEVEEYLSACDERDERMIKEVLKSDCRIFNLGDH
                     ATNEFTPPPILEKYLLPRWQKIGKIMKKNNRFVHSHWDGNSKLILKYIKISELSGIEA
                     LTPFPQCDMTLDEIKEAVGNDFVVLDLIPAIFFLPQYSTDFVVDFTKRVIDMFFPKLI
                     LGVSDELPPFGEIKKIEKISEYIYKEFGSIK"
     gene            24315..24386
                     /locus_tag="PLF90_02130"
     tRNA            24315..24386
                     /locus_tag="PLF90_02130"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24347..24349,aa:Phe,seq:gaa)
     gene            24568..>25675
                     /locus_tag="PLF90_02135"
     CDS             24568..>25675
                     /locus_tag="PLF90_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOK56116.1"
                     /translation="MFLSNFLNREKVLFLENLIELLKKINEKEEEIKNLTREKMIEKT
                     EEIRKLISEGEPLDNFIVDAFALVRESARRTLNMRHFDVQILGGIVLHSGKIAEMATG
                     EGKTLVATLPLFLNALEGKGCHLVTVNDYLAKRDTQWMGPIYHYLGLSVGCIVSYRET
                     KDRYSSVAYKFDPTHLPPDSRFLYLKPVSRKEAYLCDITYGTGSEFGFDYLRDNMAVR
                     KEDQVQRKLNYAIIDEVDSILIDEARTPLIISGPSEESPTLYYEVDRLVRKLTKDKDF
                     VVDEENQTATLTEDGIKKCEKLLNIGNLYDGTHTELVHHINQALRAHNFFKRDKEYVV
                     KDGEVIIVDEFTGRLMPGRRWSDGLHQAIEAKEGV"
BASE COUNT         9420 a         3418 c         4583 g         8254 t
ORIGIN      
        1 gatggagaaa aacaaaagaa tttccatcag gttatataat tgaaggacat gcaattattc
       61 agaaatgggg agaaggtgga agttatggga tatgtggttc aagaagttgt atgagaagtt
      121 gttttaatta ccttgaaaaa aataatataa ttgagcaaac atttaaagga ggagaattta
      181 taaaaagaga aagatggctt ttaccctata gagtcaaaaa ggaaaataaa gatgaaaaaa
      241 ttaaaggaaa aaggcaaata tgatgtagtc gttgttggtg gaggaattgc tggagttagt
      301 gcttctgttt ctgcagcaag aaatggtgtt aaagtatgtc ttattgaaaa atcttgctgt
      361 cttggtggac ttgcaactct tggacttgtt aatgtatata tacccttttg cgatgggaaa
      421 ggaaatcaaa ttataaaagg gattgccgaa gaattaatca gagtatgtgg tggtgatgga
      481 aatgcaaaaa tacctgatta ctggctttca gaaaataaaa aagaacaaag aaaaacaaca
      541 agatattttg cagaagttaa tcctttttac tcaataattg cccttgaaga atttgtatta
      601 aaagagggaa ttgaaattaa gtatgataca atagtttatg atgtaataaa aaaggaagat
      661 agaattacag atattatagt gcttgataag gaagggactg gaaaaataag ttgttcaact
      721 gttatagatg caacaggtga tgctgatata tgttattttg caggagagga aacagtttct
      781 gttgataaaa atgtcccgtg cgggtggttt ttttattatg atgggaaaaa cttaaaaaaa
      841 tatacattta ctgagcattt tgatagatat aatcaggata ttccagaagg gataaaaaca
      901 ttttcaggag tcaaagcaag tgatataact gaacaagttt tggaatcaag gagaaaaatt
      961 aaggaacttt tctttaaaat taaagaaggt aagacagatg cctatttact atccattcct
     1021 gtaattccta ctttccgcat gacaagaaga ttgaaaggaa aatttgaatt aagagaagag
     1081 gatgaaggga aaatttttcc tgatgctgta ggaataaccg gtgactggag aaaaccagga
     1141 cctctttatt atatcccttt ttcctgtctt tatggtgtta aaacaaaaaa tttaataact
     1201 gctggtagat gtatttctgc ttcaactgca tgggatatta taagagtgat cccaacctgt
     1261 gcattaacag gagaaatttc aggaactgcc tctgctatac ttgttaaaga aaaaatagag
     1321 agtttctcat caattgagat aaaaaaacta caaaaagttc ttaaaaaaca gggtgttatt
     1381 ttttaaaatt tcatttactt tttttcataa atttttgaat tccaacaaat ttattcctca
     1441 tgcttctttt gttatataca gatatttcgt gtgatataat agtttttgga taaacaaaat
     1501 tttaaaatga aaatgatatt tttgggaatt ttaatatttt ttttgaatgt gattaaaggg
     1561 ttttcttatt cagagattga tattgaaaca tttaattaca ggaatatctc agaagtaagg
     1621 gaaaaatggt tgccacagga aagaagtaga gaagtagaat taataaaaga aaaaggtaaa
     1681 aaagtaataa aatttgtttt aaatttttct gaagttgaaa aaagaagtta ctgggataga
     1741 tttgtcctgc ttgatttaag taattttgaa aaatttgtaa ttgaagttaa atcagaaaat
     1801 ccttctttaa caacaaactg cacaatttat tttgaaagtg aaggtggatg gtttagttac
     1861 agatttccaa ttgaaaataa cagttggcag aaaattatta ttccaaaatc aaaatttgta
     1921 attgaaggaa atccaactgg atgggataaa ataaaaagga taagaatttc tttctggaaa
     1981 atagatacta cagatttaaa ggaaacaagt gtttatctga gaagtttaaa aggaattatt
     2041 cctgattcaa aaataggcat tgttttctgt gatagtgcaa ttaaagagaa agggcacaga
     2101 agagatgtaa taagtaagta ttattctgat attgcaggaa tttttgataa atttgaaatt
     2161 gattataaaa cagtaaacca gagtgaattt gaaaaaaaag agattgaaaa agagtttaaa
     2221 attttaattt ttccttacaa tccatttttt tcagaagagg aaataaaaaa tattataaaa
     2281 tttgttgaaa aaggtgggaa aataattgtt ttttattcat caaatcagga aatctataaa
     2341 attctgggta ttgaaaatgc agtttacaga tatgaaagtt atcctggaga atttgatacc
     2401 gtagaatttt taccagaaat aataaatggt ctaccagaaa aaatgaaaca atcatcctgg
     2461 aatgtaatgg tccctgaaaa aataagcgaa aattcaaaaa ttgctggata ctggattgat
     2521 gcagatggaa aaacaacggg tattccatct gcaattctat ctgaaaatgg attttatttt
     2581 tcccatgtct tacttaataa agaagaagga gaacaattct tgatttctct gattttattt
     2641 tttttccctg aaaataaaaa tgcaattttt gattctctaa ttaaaaatac agggaaatta
     2701 tctggttttc aaaatttaga agaaataaaa aaattcattg aaggaaaaat gataaaatta
     2761 tcattttaca aaaaaaggga agtaaaaaat tatttaaata ttacaattag caaatacaaa
     2821 gaactcacaa aaaattacaa aaaattaaat atagaagatt tgtttaaaaa gaaaaacgaa
     2881 attgatgaga atttaagaca ggcatatttc agatgtttta aaggaaaaga aaaagagtac
     2941 agaggtgtgt ggtgccattc tccgtttggc gttaaaggat tggcatggga agaaagtatt
     3001 aaaaaactta aagaagccaa tttcaatgct atattcccaa acatgttaag agcaggtatt
     3061 gcatattata aaagcgaatt tttacctgtt gcagaagaag ttaaaataaa aggagaccag
     3121 atagaagaat gtctttctgc atgtaaaaaa tatggtattg aatgtcatat atggaaggta
     3181 aactggaatc tttttaatgc accggcagaa tttattgaaa aattaagaaa tgaaaaaagg
     3241 ttgcagattg ataaaaatgg aaaagaagtt ctttggctct gtccttcaaa tcctttaaat
     3301 tttaaacttg aatttgattc aatgcttgaa attgtgaaaa agtatgatat tgatggaata
     3361 cattttgatt atataaggta tcctgatgca aattcctgct tctgtgaaaa ctgcaagaaa
     3421 aattttgaaa gggaatataa tgttaaaata gaaaactggc cttatgatgt gataaacgga
     3481 aaatatatgc gactttacaa aaaatggagg caggaacaga taacaaaact tgtaagagaa
     3541 gtaagcaatg aagcaagaaa aataaaacca aatataaaaa tttcagcagc ggttttttca
     3601 aattatcctg attgtaaaga aacagttgca caggactgga aattgtggat tgaagaagga
     3661 tatcttgatt ttatctgtcc tatggattac acaatttcaa actatacttt caaaaattat
     3721 gtaaaaaatc aaaatgaagt aatcaggaaa aaaatctctt tctatccggg aattggtgcc
     3781 tatattttat ctcctgaaga tattgtaaat cagattgaaa tttgtagaga aattggaaca
     3841 gacggattta ttttgtttga ttacaatttc accctttata aaaatttata ttattttggc
     3901 ctcggtataa ctgaaaaata aaattttgac gaaaagataa aatttatgtt taaataatta
     3961 taatggaaaa aataagagac cctgttgtta gtggttattt ttatccatca gggaaggcac
     4021 atcttttaag aatgcttgaa acatttattg atagaaacac acagaaagaa aaagtaattg
     4081 cctgtatggt tccacatgct ggttatattt attcagggaa aactgcaggt ttaacctact
     4141 caaaaattga aatacctgaa agtgttataa ttttagggcc gaatcatacc ggctatggag
     4201 aaccatattc tgtaagtgac catgataaat ggctaactcc tcttggtgaa attgaaattg
     4261 ataaagaatt gagagataaa cttgttgaaa aaagtagata tttacagaaa gatgaaattg
     4321 cacatataag agaacattca atagaagtcc agattccatt tttacagttt ttaaagaaag
     4381 atgtaaaaat tgtacctata actctaatgg gatatgttga taatcctgca tggtatgaaa
     4441 taggagaggt aattgctgaa gttataaaag aaagtaaaaa ggaatgttta ctaattgcaa
     4501 gttctgatat gacacattat gaaccacata aaattgctga aagtaaagat aattatgtta
     4561 ttgaggcgat atgtgattta gatgaagatg aagttataag aagaatagag gaaaaggatg
     4621 ttactatgtg tggttattca ggagttatag taagtattgt tgcatcaaaa aaacttggtg
     4681 ctgaaaaagg tcaacttgtt tattatacaa caagtgctga aacttcaaag gactattcac
     4741 aggttgtcgg atatgcagga gttataatta aataaaagtg tttttctcaa atttattttt
     4801 tttaatttta aacagttttc tgcttgcagg aatttttgga gtttctggta taatagaatt
     4861 ttttgttatt tttttaattt ttttctactt tcagatttac ggtatttcaa taattcttgg
     4921 cctttttaaa attctttatc ctcaatttat tctaatcttt cttttcctca tatttctttt
     4981 tcttcttcca gaaataaaaa aaataaaatt gcctgattta aaaaatatac cttttaaaat
     5041 ctctattttt ttatgtttaa cagtttcatt acatcttctt tatattttta atcttttgat
     5101 actcccccct ttaaccacag atggtcttct atatcattta ccatttgctg ttcattatta
     5161 caaaacccat tcaatttcct taccaaacct ctattttaca gaaatttcaa tgacatatta
     5221 tcctattgga ggagaaattt tttacttttt tagcattctg tcaaaaaaag aattcctttt
     5281 taaatttact caattcccat ttctaattct tggttgtttc tctgtttttt taatttcaaa
     5341 atcatttggt ttatctgatt ttctttcaac tctctctgct atttcttttt cacttataaa
     5401 acctgttttt aaagaatcta caatgtgttt tgttgattta atgatggctt ctactttcat
     5461 cacaacttat tattttttta acaaaaaaga aaaaaaatat atatttcctg gacttttatc
     5521 cctttcttta cttttatcaa taaaaacgtt atctttactt tttgcacttt tacttttacc
     5581 ctttcttttt caaaaaaaat ctggtaaatt ctctaaactt ttgtattttt ctgtttttta
     5641 tcttttattt tttggtctct tttcttactg gcgtaatctt ctattaacag gtaatcctgt
     5701 ttatcctgca gatataactt taggaaactt tactttattc agaggtgctt atatttatca
     5761 aaaggtttca attattgaaa aaattaaaaa tttattttct gttcttgctt tctcttcatt
     5821 acatattgac ccttctttca atttaaaaat tgttcttctt ttatttttcc tgatttcttt
     5881 tacactctca ataagaaaaa gagagatttt aattttttac tttttatttc ctctttccat
     5941 tcttttatat tttttattaa tcccttctac ctactttcaa ataagacacc ttttgcctgt
     6001 ttatggaata ttatctattt ctcttttcta cccatttcaa aaattggaat atttatgcat
     6061 tgctgtattt ctgtatttct tcctttctgt ttttccatct tcctgtattt ctttgttttt
     6121 gataatcttt tcaatacttt ttatttcatt ttttataata aatcgtatta aaaaacctgt
     6181 tttttatgtg ccccttttac ttctttttat tctatttctc atatttcagg ttgatataaa
     6241 aacgcctctt tacaaagaaa taaagtttga attctggaag agtttttata aggaagaagg
     6301 agaaatatgg gaatttgtac agaaaaattc aaaagaagga aaaaatattg cttatgttgg
     6361 aagttttttt atatatccat tttatggaga aaattttcag aataatgtat tctatcagtc
     6421 agtaaattca atcaaaacaa ttcctgtgta caaatataga aaaagaataa agtatgagga
     6481 accatcagaa aatttgtata gagaaaaccc agattttaaa aagtggattg aaggacttaa
     6541 aaatgaaaaa actgactgga taataattaa aaaagataaa gaatatattg aaaggaaatg
     6601 gatagaggaa aatccagata tatttaaaat cctctattca accaactatg ctgaaattta
     6661 caaattaaaa attgaatagc cagaactttt acatattaaa tttgtatatg atgctttgta
     6721 tatattattt tttttttaaa aaagttgaca aattaaaata aaaaggtata atatttttgt
     6781 atgaaaaatg ggatgcctga ttatttaata atagaaaaag aattaagaga aaaaatagaa
     6841 aagggggaat acaaatcagg agaaaggatt cctaccgaag tccaactctg tgaaaaatat
     6901 aaagttagca gaattacatt aaggaaagca ctttctatcc ttgaagcaga gggttatata
     6961 tatagaatta ctggtaaagg gacttttgtt attccttctg aaaaaaggga tgaagcaaaa
     7021 atcataaagg aaagacagaa agaaataaaa aaattgaata aaggagttgc tattcttgtt
     7081 ccttgtataa catttactat tttttctgga attgtaagag gagctgagga ttacttaaga
     7141 gaagaaaatt atcatttact tcttgggaat tacgataata atccagaaaa ggaaagagaa
     7201 tatatggaaa tgtttacaga aagaggcatt gagggtttca tagtttctgc aaattatttt
     7261 tctaatcaaa attcttatta tgaggaattg ataaaaaaga atataccttt tgtatttgct
     7321 gatacccccc ttgaaaaatt taaagcagac cttattgcaa caaataatat aaaaggtgcg
     7381 tatcttggaa caaaatattt gatttcacgc ggctgtaaaa aaattgcatt tctgagtgct
     7441 ttaatgatac tttacagttc tgaaagaaga tttatgggat ttagaaaagc aatggaagaa
     7501 ggtggtctta aacctgatga aaaaataata aaacatggat atgcaacaga agaatttggt
     7561 tatgattcaa taaaagaaat tatgaaaaaa gaaaaaattg atggtatttt ttctgcgaat
     7621 gaaccaataa ctgttggaat tgtcaaggca ctgaaagata tgaatataag taaagatgat
     7681 ataaaaattg tatcttttga ccagccagtt cttccaaatg gaacagacta tgatgtaaaa
     7741 cttataaaac agccaagata tgaaatagga aagatatctg caaaaatact acttgaaagg
     7801 ataaaagaaa agaaaaaagg gataaattca atttataaag aaatactttt agaaccaata
     7861 atagaataag gaggtgagaa aaatgaagaa aaaaatggga tttactttga tagaactgct
     7921 ggttgtagtt gctattattt ctattctggc agcattaata ttacctgcat tagaaaaggc
     7981 aaaagaaaag gcgcagaggg ctgtatgcat gtcaaattta aaacaattgt atattgctct
     8041 ttccctttat gcaaatgatt atgatggtta ttttcccata gcattagaaa atcagtataa
     8101 tagaataagg acatcccgtt caatgatgtt acttcttggt atttatgttt caggacctgc
     8161 tggcacttct cctccatcag gaccacctac aggaccaaga tatgtaacaa atcctgaatt
     8221 gtttatatgt cctgcttcaa gggataaagt ttcaaaaaca ggatatcttg tatatcctta
     8281 ctgctcatat gcatatgcac caaatttaaa cactaaaaaa cttaaagttc ctgctggggc
     8341 aatagaaata ggaacaggaa aaacagatac cctgtcttca aacaactggg caattatgtc
     8401 tgataaaaag tatgtagatg ataatggctc ttttgaatgg tatagaggat ttggttctta
     8461 ttgggaatac agatttcaac ttgtggaact ttgcaatcac aaaacttatg gaataaatgt
     8521 tctttacata aatggagatg ttcaatggat acctgctttt cagtggcttg gtccaactgg
     8581 atggacaaaa tctgtgtcac agggtggaaa ttactatata aatggaaaat ggtatattcc
     8641 ttctgaaaaa ataccgaata tcaactattc aatgggagga agtcaacctt actggtacac
     8701 aagtaataca ggaagtagtt atgttcatcc agaccagcag atttatttaa gaggacctga
     8761 aagtaccggt aatttttaag gaggagaaaa atggacaaaa gaaaattttc cggtttcaca
     8821 cttatagaat tacttgttgt ggttgcgata atagcgattc ttgcagcaat gcttttacct
     8881 actttgagta aggcaagaga gagagcaaga agagctattt gtttaaataa tttaaaacag
     8941 ataggtcttg cttttagaat gtatgcagaa gattatagtg gctggcttcc gaattatgaa
     9001 ggtccaccaa caagttcctg gtatgtttta acgtacagac cttttaacaa gttgattgga
     9061 aaagattctt ctggaaacct tggaggagtt aaatatgtga caactgcaga ggtttttctc
     9121 tgcccaagcc aacgacttga ttacagaaat gtctcatctg tcagaaaaac cggatatctt
     9181 acgcctcagt atcaactttc ttatgcttat gcaaaaccat atgatataag tacatgggcg
     9241 agagtcagtt acagtataaa tccttatgca tatgtccctg gtcttggaat taacgcagga
     9301 acagagccgg attctatact tgctgctgac agacagagac ccgatgctac aaagggaaca
     9361 gggaatctag gtggtggacc gctttgggtt attgatagat gggattgggg tggtgcaaca
     9421 ggaacagaag accagccagt agcagaaggt tctatcccaa aaggaataac actgacagaa
     9481 gaaaataatc atggaactgc aggtattaat gttttatata tggatgggac tgtgagatgg
     9541 attgctgcta caaaaacaga ggtttccgga gagataaagt atattttacc aatgaaaagg
     9601 aattatgaag gaataactca atgggttgga aggatgcata atcctttaat ttatgctcct
     9661 taattaatgg aggtgaatca tgaaaagaaa tggctttaca cttgttgaac ttcttgtagt
     9721 aatagcaatt atggctattc ttgcaacttt aattcttccc caattggcaa aggcgagaat
     9781 cagagcacaa caggcagcat gtataagtaa tttaaaacaa ctcggtcttg ctgttcgtat
     9841 gtatgcaaat gattatgatg attgtatacc ttatatgaat ggagatgact caggcagggc
     9901 ttataacaat catctttctc ttttactgcc atatgttgga tggaaacaag gaacaacagt
     9961 aactcctgaa ttatatgtct gtcctgctgt tggactttca ggtgcacctg atgcaacata
    10021 tagttgtttc ccctttacga atgttaaaat taaaaggata tattgcttca atagaataac
    10081 aggaggattt ccttctgggt atggtaaccg tgtttataat ggacagaaac tcggacatgt
    10141 accagaccct acaactcata ttctttttct tgaagggact tgcgaccatg tttatagttt
    10201 aagccagata atgtatcttc acggaaataa tacgaatgtt ttatttgcag gaggaaatgc
    10261 aggttcattc ccaaaaggtt ttcttgaaaa tcctgcaaat cttgttcctt actttggaca
    10321 aaaattggca ggatattgag aaatcaaaaa ttggaggtaa ataatgaaaa aaatattttt
    10381 atctacaata atagagtttt ttctcttttc ttctttttta ttgttttctg atacaaatct
    10441 atgggaagga aaaagttctg actggtttga taaggaaaac tggtcaatga aaaaagtccc
    10501 tgataaaaac gataataatg atgttcttat accttccgga aaacttaatt accctaaact
    10561 tacttcagat gtatttatag acggaactct tacaatagaa aaaggtgctt caattcaact
    10621 gaatggttat aatatcacac ttgtaaatct tggagtaaac aataaaaagg ggattgtaaa
    10681 taagggaata attaatgctg aaaagaatgg ttctattttg actgttggaa gaggggggtt
    10741 cattaatgaa ggagaagtta aaggctatcc ttcccttgtt tttggtacat attcatttga
    10801 tgcattactt aaagcagggg gtgcaaattt ttcttcaata accatttctc ctggttctta
    10861 ttcatacaga gtaacagcag tggataaatt agtaattaaa ggaaatttgg ttttacaggg
    10921 acactcatta tatgtgaata aaaatgcttc tgtttatata tatggagata ttatttttga
    10981 agggaagtca ccaagaactg caggtatgac ccttgaaggg gatgtttttc tttatggaaa
    11041 tattgtaagc aatgattctt ctattgggaa tggaaacgga aatggctgga taatttttaa
    11101 taaggatgga ttgcaggaaa taaatatttt aaaagaagga ggaattttac ctcctataaa
    11161 aatagaaaaa aaagaaggaa ttgtaaaatt aacaggaaat ttatcatgtg ctggtttata
    11221 tattgaagaa aaaaatgctc ttttgcttaa taatactgaa aaaatacaat ttggtggtgc
    11281 tgggtctgaa agtgcataca ctcgtggatt gataaataaa gggaaaattc agggcaagac
    11341 agatttaaat ttttcaacag gatgttataa tgcagattta ttgcaaggag attttgaaat
    11401 tcagaattta aaaattgata ctggaaatta tgcatattct gtcaatttaa aagagaattt
    11461 gaaggtaaac ggagatattg attttaaaac aggaacttta aatatgggag aaaacgcaat
    11521 aacagttggt ggaagttggt atttttctcc atttccggaa aaaccctctc ctgttactcc
    11581 tgttctacaa tcaggaaaaa atgcaaaaat aatatttacc tcaaactcaa acgcaataat
    11641 tcaatcaggc ggtgttgaaa aagcacatta ctatgacgca acattcccaa atttaataat
    11701 aaacaaacca gaaggaattc taataattaa agaaaaacct cttgaagtaa aaggaatgct
    11761 gaaacttgaa aatgggaaaa tagaaatgaa agatggagga gaaatatatt tgggagtggt
    11821 aagagaccag acatatggga aatacagaat ctttgatatt ggacttaaat taaaaggaac
    11881 tctcacaaaa gagatgtttc ctgaaatagt ccctgctaaa atacctgaac ctactcctgg
    11941 aaaagtaagg attattcaca attatgttgg acctgatatt tcttcaaaag aactttttaa
    12001 tatagcacct tttgcaaata taacaacaat tccttataca acaatagtta aaaaccttat
    12061 agataatgac cctgacttaa gaactttaac tttacctgaa acaggaactc cagcaaaatt
    12121 ttcaagatac cagtttcagt ttaaagaaaa acaaaaaatt tcaagaattt catggtgtgt
    12181 agcatcaggt ccatgggcaa ttcttgctga taaagatgga gatggaaatt gtgaaacgct
    12241 tttaagaata gatattgagg ggaaagtaga aaatccggga ggaacatggg tatcaaggtc
    12301 ttttttgcat aataattttg tccctcctgt tgaaacttat ggaataatgt atgttgattt
    12361 ttcagggaaa aataatattt atgattttca gatacttata agcgataaaa aagattatga
    12421 taaattaaaa aaagattttg aatatgaaaa agaaaaagga gtccctattg ttgaagatgg
    12481 ggaggagata aatgttcctg ctccttctga aaaagaaagg atttttacag gttttcatat
    12541 tgaaccatgg atgtttaatt taccccaatg gataaagatg gataaagata aaagaccttc
    12601 tctgaatgaa tataaagggt tcagagattt tgttgaagga gtgaaaaatt ataaagggaa
    12661 tattcttaat atgtggccac caacaacatg ggagcaggtt gggcctggaa catatgaaca
    12721 tccagttctc tggccaagcg aatatgatag acacagtgca aaagaaaatt ttcttgaaga
    12781 agttgttgaa gcatttcata aagagggtct aaaactcttc acaatgagaa gggttgcata
    12841 tccaaaaaaa ctggaagaat ttcccaagac cccaacaaga gacaaacctg caccctatat
    12901 ttcaagacat agtagagaat atttaaatgg tatagttaga gaacaggttc tttcaggagt
    12961 tgatggtgtt ggtattggtt ttgacgagca gatgccttac agaactattt taaatttaaa
    13021 aggagtgaat gaagtaacaa aaagtatttt taaagaaaaa tataaatgtg aattaccaga
    13081 agaaccgcag gatacagaag catttcgtaa atggattgtt tttggatatg aagaatttgc
    13141 aaattatctt gctgatgctg caaacgaagc aaaaaaaata agacctagtg tttatacaaa
    13201 atcgcctgtt cattttaccc ttggaacatt atggaacagt cgtattgatg ttggaatagc
    13261 agaagatatt gtaggaaata aagccagaat agattttgca agaggtaatt catatcttac
    13321 atttcaatca cttggtcatt atgtgactgt tttatctgca aaaataggag taggagcaaa
    13381 tgaaaatggt ggagttgttt ctttacacaa ctgctcatgg tcgtggagag atgatactct
    13441 taatgttcat ccaggatttt atctccactt ccctccagtc tatatgaggg catctcctat
    13501 aagttctgta ttaaatggag cgagaatgac tttattctgg agatataatc ttattttttt
    13561 tggtaattat gataaatatg ttaaggaagg atattccatt attgaaacta tgagtagttg
    13621 gggagcaaag gaagcaaaaa ctccaaaata tatagcaatt ttaaggtcaa gaacatccga
    13681 ggactggtgg caggtaaaac agagatataa tgaaaaaggg gacccgatgg accaaataag
    13741 gggctatata tatcttaaat atgtaatgga atttctattg agaaatgggt atccttttga
    13801 tgtatattat cttgaccatc cagttgaaaa tttaaggaaa aaacttgacg gttataaact
    13861 tttcattctt cccttccctt acagtataag taaagaaaca ttcaatttaa ttaaagaatt
    13921 tatggaaaaa ggagttggag tgattttatt tgacagaact ggtgaaactg atgagtgggg
    13981 gaatttatat gaaaaagggc tttttgagga tttaattaaa gaaaaaaagg tcttatttat
    14041 aaatgaagac ataccaaaaa taggacatca tcctgacttt ataaaatctt tgggccaaaa
    14101 aatagatgaa acacttggaa gagaaaaaac attttatttt aattcttatg gagaggatgt
    14161 tgaatttggt attcttgaaa ataaaaataa agaatacatt ttaactttca taaactggac
    14221 ggataaacct gttgaagtag attttggact tctaaaactt cctcaaacaa aaaattatga
    14281 aattttacag agagatttaa atggaactaa aagagtaaaa attggaaata aagagttatt
    14341 aaaaggaatg gatttgaaaa aaatacgaat tgctttagat aaatgggatg taaaagtcct
    14401 ttatgtaaaa taaaaaggga gtaaagatga aaaaaataat tgaatttgct tgtgttttat
    14461 taattttaac ttgtttttct ttaaaggcag aaagggatta taaacttgac tggtacaact
    14521 gggtagaagt aaagactgct ccttatatgc ttaaaataga caaccttaca gattctgata
    14581 caaaaactgt ttctgaatat cttggaggac ctgagatagg gggatggatt gaatggcaat
    14641 tcccttctcc tgtgaatgta agttatgtaa agtttatgca gagtggcgca gatacttttt
    14701 ctctaatgtg tgatacagac ggtaaattac tgtttaatat tgaagttgga aaagtaaaag
    14761 tagaaaaacc tgatgaatgg ataataattc ctgtcaataa aaaagttttt ggcttaaaac
    14821 taattgcttt aagtggaaaa gcaggatacc gtactccata tccaacattt aaagagattg
    14881 aaatttatac aaaagaaaaa ataactcctg aaaaaaagag agaggaaaaa agtaaagcaa
    14941 tactcaataa aggaaataaa attcagtttc cagagttaaa caaaaagaat ataaaattta
    15001 ttccatgcat agacctctgg atggcaggaa ttattcctgg tgataaaatg caggaaagta
    15061 cagaggaaat tgaaaaaagt aatggattcc aaacgatgct taaaaaatta cgggaaatag
    15121 atgctcaggg agtaagaatc tttcttgaaa cttactgctg taataataaa atgccttgga
    15181 aaagtcaact tgctccaaat tatggaaagg atactcttaa aggatttata gatgcactcc
    15241 ataaaaataa tcttacatct gcaattttcc ttcatgcatg gatttctcct attcaaccag
    15301 cagaaaagat gttaccaatg cctgatagaa gatgggatta tccttatgaa caaagtgatt
    15361 tagtagcaag taaagggtta tacgaaggat atgaaccaaa tagatatcct tgtgtaatat
    15421 gtgatgatga cttcaaaaca aaatggtttg gtctacttaa agaggttgtt gaaagaggaa
    15481 tagatgaagt ttatgttatg cctgatgaat attactttaa aggacataat cttgaaactg
    15541 taagatgtcc ttcatgcgaa agagaattta agaaaagata tggatatgaa agtttgccaa
    15601 aagttgggaa aggaggagga attaaaagag attttggacc tgacccgaga aaaatgatag
    15661 ataatgaaca ttacaggaaa tggaaacttt ttgaatatga aaagattgca gaactgttta
    15721 acgaagtggc tgataaactt aaaaaactga atcctaatct gaatttaata agtagtgcaa
    15781 atcctgcatc ttctttatat tcaaatttag gacttgaaca tggaatagca tttgatatca
    15841 ttggcagagg aaaaagtttt acacatattc aactttatgc acatgttccg ttaaattatg
    15901 gtggatatac agcacttgca agaagaatgt ctgctgctta tcctgatgca aatctttctg
    15961 caagtatcca gtcgctcggt tttaaacacc taaaagcaga caaaagtaat gaaattttgt
    16021 tctatggata ccttattcct ttcattatga atggagcaac gagaatagac ctttaccgtt
    16081 tgaattatgt ggaaggaaat ggttggtggg aaaggataaa aaatggtata aagatggtaa
    16141 gattacttga agagtgggga attactgaaa gcaaaagtgt ggcagagaca tgtcttctta
    16201 tttcccgtgc ttctgaggac tggtggcagg taaaagctca aagcgcaatg ggaactgttg
    16261 aaccagaatc aaaaaggtca accatacttt atatggaaga aaaacttgga actgtccaga
    16321 cagaaacaac agatgaaatg gaaaggacac ttaattttga aagattcagg ggaatgtatt
    16381 caaatcgtgc aattgaatcg gttttaatag aaaacggaat tcagtatgat gttaaatata
    16441 cagaaagaga agaaacttta ggagatttga gtaaatataa actccttatt ctgccattta
    16501 gttattcaat gtcaaaaaaa gcatatgaaa atatcaaaaa agcagttgaa tcaggaacta
    16561 aacttgttat attcaaccaa cttgcaccaa ccaatgaata tggagaacca tattcagaac
    16621 ctcttttaaa agaactcctt aataatccaa acgtgaaatt tatagatata aaccctgctt
    16681 cagaaggtat gaagaggtct ttcaaaaaat tttatggaga tttattttac aaactgcttg
    16741 ggacaaaata ttactttaac aacaatgata ataaagtgga atatgtttta agaaagatag
    16801 ataataagaa ttttattttg taccttgcca attgggataa aaatgaaagt acatgtgtaa
    16861 ttggtatacc tgttgcttcc gggaattaca gaattttgat ttacagcagt ttaacagacg
    16921 aattaagaga aggtacaatg aaaggaactt ttgggaacaa aaatattatt aagggttata
    16981 gttttaagaa ttttggaata aagataagcc caggagaagt acttcttgta aaaatctatc
    17041 ctgaaaaatg aaagaggaaa aaattgtttt aaaaaatgat attcttaaaa ttgaaataga
    17101 taaaaaggat ttctatttat ctgtttttga tttaagaaca ggtataaaat ggctaatgca
    17161 aaaaaatggt cctggagata tagggataaa aggtcattca ggtccgtgga taggtcttcc
    17221 tttcagttct gcaggaagtg ttgaatggaa agaaaatacc aattttattg aggtatggtt
    17281 aacaaggttt ccttatagag caaatatatg gggaccaatt gactttgctg tttttttaat
    17341 ttttaaactt atggaagata ccctctcaat catagttggg acaaaaaatg gaagaggtga
    17401 agtttcctta atagacagtt actatccgag aggtttttta tttcctgaaa aagtaaacgg
    17461 attccttgtt cttccttatg ggcatggatg tcttcttgat aaaaaattcc cttgcgaatt
    17521 agaccatacg tttcctgttt atgcaggaat gagttttgtg atgccttggt ggggacagat
    17581 aacagaaaaa ggaactggac ttattgcttt accagaaacc cctgaggata tggggataag
    17641 attaataact gaaaatggtt atggtcacac tgctcatcca tactggcagg catctcttgg
    17701 aaatttttca tatccgagaa aaataaatta taaatttttt aaaaaatgtg atgttgtttt
    17761 acttgcaaaa acttacagaa aatttgcaga aaaaagagga attaagggga ttttaaaaga
    17821 aaaagcaaaa gaaagaaaaa atgtagaaaa acttaagggt tcaattattg ttagtatatg
    17881 gcatgagtcc tggtttggca atttcagaaa aaaagatgta tggagaagaa tgacttttgc
    17941 agaaggatta aaaagattca aaatgcttgc aaaagatgtt ccaattaaaa aagcagttgt
    18001 tcatattgat ggatggggaa taaaaggata tgattgttgt catcctgata ttttacctcc
    18061 tgacccaaaa cttggtggat ggaacggact aaaaaaaatg gcagatgaaa tacaatcaat
    18121 gggtcatata tttttattac atgataatta tgttgatgtt tatgcagata gtagtgcctt
    18181 tggtcctgaa tatacagttc ttgatttaag cggggtaaga cccgagaata acgaatggct
    18241 tggaggaaga cagagatggc tatgtcctaa aaaaacaatg aaatttgcaa aaagaaatct
    18301 aatggaggtg aaaaaaaaga ttaatccatc aggaacatat cttgattgtt ttacatatag
    18361 tcatttaaga gaatgttatg accaaaaaca tctttcttcc cgcaaagata ctataaaata
    18421 ctggtctgaa atatttgcat tatgtcaaaa ttttggatgg gtaacttcct ctgaaggtgg
    18481 agcagactgg tcaatacctg tacttgactt ctgccatact gttcagcctg gaatatgtcc
    18541 atttgacctt agagaaaaat taaaaggacc ccttggaatc cctgttcctc tttattctct
    18601 tgtctggcat gactgtatag taatcccagc ttggatatcc ggtgaaaaag acactgattc
    18661 attcttatgg ggtattttat ggggaggaat tccttctatt cgtacaaaat ctcttgaatg
    18721 taattctgct ccttatggca aagaagatat aaaatttgtg agaaacctta taccaatggt
    18781 agaactttct gaaaaaatat gttttgaaga aatggtcaat tttaaaattc ttgattctaa
    18841 cggaaatatt cagcaaacag aattttctga tggtacaagt ataactgttg attttaagaa
    18901 taaaacttat tggataaaaa aatgacaaaa taccagttaa cacgaaaagt taaaaaattt
    18961 gttttggata acggagcaga tctcgttgga attgctcctg ttagcagatg ggaaaaggca
    19021 ccaattaagc acagcccaaa aggtattatg ccagaatcaa aatctgttat agttgcaggt
    19081 gtacactttc ttgatgcaaa tacagaactt gcagcagaaa aagaccctcg tttcccaggt
    19141 ccaggatatt cagaaatgaa tgcttctgtt tttctacaat cccttggttt taaaattgca
    19201 aaatttctgc aagatatggg attctctaca atacctatta ttcatacaat gctctggaat
    19261 tacagaccac aggaaggagc agaaagagga tggatggcag atatgtgtca ttattatgca
    19321 gctgtatgtg caggccttgg agaaattggg tggaacaatc tatgtttaac tccccaattt
    19381 ggaccaagac agagatttat atccattatt acagaagcag aacttgaacc agaccctcta
    19441 tataacggag aaccattatg tgataaatgc cttctctgtg ctaaaaactg tcctaccgag
    19501 tcatttgata aagaagttat aagaattgcg acaatagaaa ttgaagacaa aaaattttct
    19561 ttcccttaca gaaatttatg gcgatgcgca attggagaga attttcaact tgatgttttt
    19621 atacctaaac ccgaaaagat agacgaaaat tttattcttg aaacaattga cagggggatt
    19681 aaagaaaaac cagaagtatt ttatggatgg aaaatgggaa tgtgtcttaa atactgtgtt
    19741 cctcctaaga gaagatattt tgacaggaat tactgcaaat ctccaaggag aaaaagagat
    19801 aaagaacccg atttttctga agaaaatgtt aaaaaactta taaaagaaat taagaaactt
    19861 gctttaaaac tggatgtgga tataatagga gcggcttcaa ttgaagaatt taaagaaagt
    19921 ggtataaatt taaaccaatt tcttccggat gcaaaaagtg ctctgatttt gggtttttca
    19981 tatcctgaaa attgttcttt aaacacatgg tttactgcag aagttgccga acttctaatt
    20041 gcaaaacata tacaggaaaa atatggattt tcttctttga taaaaagtgg aattgatgat
    20101 gaaaaatgta tgaaaatttg taatcttact tattatagta aagggattac tttttctgaa
    20161 aaaaataaaa tcttaagaac cataattacc caagcacctt ttaacaaaaa ttctttcaag
    20221 tcaacaaaaa agagagaaaa aaagaaaaca ctttccacct cttcccttac tgaatatatt
    20281 aaaacttttt ctctaaaaaa tggtgctgac cttgtcggta taacatgcgt ggaaaaactt
    20341 gatgaaatag cagaacaatt aaagaatata tatgaaaatg aaaaatattt tatcgtggag
    20401 aataaaggtt gggtatgcga tggaaaaata ataaaagatc tcatgcatgc cccttttaat
    20461 ccagttgtaa aggaagttaa acttatcccc agaaaacctt ctgattattt gaaatctgca
    20521 aggtctatta ttgttattgg tttacatctc atagaaaaaa gtattgaact cgctggttct
    20581 cctccgggat ataaagcagg ccactatatg tatacaactc atatggaatc aataaatcaa
    20641 atttttacaa tactgaaaaa aattacaaga gaactttctt cctttggata tcagtgcata
    20701 ccagttattg atatatttga catttcttca aaaatatatg gtgaaatacc tgatttatat
    20761 tcatcacgat ttgcagcaat tgcttctggt cttggagaaa ttggatggaa tggaattgtt
    20821 ttaactcctc aatttggacc aagacaaaga tttgcctgca ttattactga tgctgaatta
    20881 atccctgact ctgtctataa aggaccttct ctttgtaaaa aatgttatga gtgtgtaaat
    20941 gcctgtcctg tatctgcaat ttcagaaaaa gataaaaact ttataaaaat tgatggaaaa
    21001 gtatttgaat ggggaaaatt aaatattctt agatgtgatt gtgccaagag atatgctctt
    21061 attgctgacg aggggccaaa atatattggt tctaaaaata attttaaact acccgaaaaa
    21121 ataactcctg aatttgtatg tgaagcatta aaaaagtcag atagaattca agaaccaggt
    21181 tattgcacaa ttgtagaaaa ttgttttaaa atatgtaatg gcaggaaaaa ataaatggaa
    21241 agtaaagcaa aggaattttt aaaaagactt gttgaaactc caagtccctc tggttttgaa
    21301 agaattgttc agaatcttgt taaagattat ctgaaaaatt ttatagacag aatagaaatt
    21361 gatatccatg gaaatttaat cgccattaaa aatccttccg gaattcccaa aattatgctg
    21421 ggtgctcact gcgacgaggt tggttttcag gttaaatata tatcagaaga aggtttcatt
    21481 ttttttcaaa caataggagc gccttatata cactcacttc acggacagaa agtcataatt
    21541 ttgaataaaa aaagatgtgt taaaggtgta atttgcaaaa aaaagataga taaagcagaa
    21601 gatttaaaaa taaacagttt ctggatagac ataggtgcaa aaaataaaga agaagcagaa
    21661 aaatttgtca gcataggcga tccaatcgcc ttttcttctt cttttacaga attgcttgaa
    21721 gggaaaaaca taatggcaaa aggatttgat gataaaatag gagtttttgt tatcgcagag
    21781 acaatgagat tattgagtaa ggaaaaaata aaatcttcag tatatggagt ttcaactgtt
    21841 caggaagaaa tcggttcaag aggagcatgg atggcttctt ttaagataaa accagatatt
    21901 gctatagttg ttgaagtaat ggaatgttct gattatcctg atacagaaaa aagaattatg
    21961 ggtgatataa gattgggcaa agggcctgta ttacagatag gtccaaatat aacccctact
    22021 cttggagagt ttcttattga aacagcaaaa aaggaaaaaa ttccatatca gatagatgct
    22081 gctgccggtc caacaccaac agatgcgagt gtaattcaga taatacaggg aggcattgcc
    22141 actgctttat tgagaattcc ccttagatat atgcatactt ctggagaaat agtttcccaa
    22201 gatgatgtca aaaatgcaat aaaactttta actctttcta taaaaaatat gttttaaaaa
    22261 ctaaattttt catttggtaa agtaaaattc tattatgtct ccgtcttcta tatgtgaatt
    22321 tggtccaaga agttttaaaa tccccatatt ttttgcctta tgccagtcag ttatttcaac
    22381 aaactcatta aaacttaaag atactgcctt tacaaaacct ttctcaatat ctttatgaat
    22441 cttccctcct gcctgttttg catttaagtt ctctggaata atccatgcct gtgcaatatc
    22501 tccttttatt gtataaaaag ttatcattcc aagtttttct attactttct tatagatatc
    22561 ctttatttca taaataactg gaaggtctgt ttttatttct tttctattac catttgataa
    22621 aattaaaaac tctttcaatg ttataagttc tgctccaagt aattgttttt tatctatttc
    22681 catatccttc aaaaattttt ccgattttaa aaaatcaaga catttttccc agatttcagt
    22741 attagttttt tcttttttcc taccttctat tatactttca accctttcta tatcatgata
    22801 aataagttcc ataaccagtg attccatttc ttcctctgga tttgcttcct ctgcgaaatt
    22861 ttttacaatt aaaagaataa tatctgcttc aaaaaagttt ttaaaataat tagaaggaaa
    22921 tccttctcct tctggaaaac ctttaaaatc atatatctca aattcaggat agacaacttt
    22981 ttttggcttt acaatttctt ttatcttttc aagtcgctca tctttgtaaa taccaatccc
    23041 aatattcggt ttaaacgggt caaaaatctc gctttctttg cctgtcagtt ctttaaatat
    23101 atatgtcttc ccgcttcctg aataacctaa aagagcaatt tttatcattt tatacttcca
    23161 aattctttat aaatatattc agaaattttt tctatttttt ttatttctcc aaaagggggt
    23221 aattcgtctg acactccgag tatcaatttc gggaaaaaca tatctattac tctttttgta
    23281 aagtcaacaa caaaatcagt tgaatactgg ggaagaaaga aaattgctgg aattaaatcc
    23341 agaacaacaa aatcatttcc aaccgcctct tttatttcat caagtgtcat atcacattga
    23401 gggaaaggag ttaatgcttc tattccagat agttctgaaa tttttatata ctttaatatt
    23461 aattttgagt taccatccca gtggctatga acaaatctat tatttttctt cataattttt
    23521 cctattttct gccatcttgg taagagatat ttttctaaaa ttggaggggg tgtaaattca
    23581 tttgttgcgt ggtctccaag attaaaaatt ctacaatcag attttaaaac ttcttttatc
    23641 atcctttcgt ctctttcatc gcatgcggat aggtattcct ctacttcttg tggataatct
    23701 ttaaggagat aatatgtatt caaaagtcct gcataaaatt taattaaatc agtaaaacca
    23761 gaacttgaaa gaaaaattgt aggtgctccc ctgtttccca ttcttttatc tctttcatca
    23821 aaaatttctt taacaaaaat atatctttta tttcttatta aatgtgttaa aatcttcagt
    23881 tctttaatat tttcacaagg aaattttact attcttttat tttcattttc tctgacaatc
    23941 tgataaacag tccttaaatc accccatttt gttcttacta cactttctgt atggttttca
    24001 tctaaaattc tttcaaaaaa ttcaacaggt tcctcattat attcaaaaag accacttgaa
    24061 gcaccatatc tgacagaaca accaagttgg tcataaattt gaaagtcaga aaggtcttta
    24121 aaactatcag gcaaagaatt attttttctg tgataatata tccaggtttc aagtctgggt
    24181 tgccataaaa ctttatttgc tttcccttca aaaattttta tattcaattc tctttttgtc
    24241 atttttttta attttaaagc aaaattttaa acttgacaaa aaattcataa aagggtattt
    24301 tattcactat cagagccggg gtagctcagt ggtagagcgc agccctgaaa aggctggcgt
    24361 gggcagttcg attctgcccc ccggcaatta caaaacagat aaaatgttgg gaaaattttc
    24421 tgatttctat ctgaaaattt ttgggtggga agaggaataa attttaattt taaaaattgc
    24481 tgaaatttcc atccataaga gaggtaaacc atgaattatt atgaagattt actatccaga
    24541 ttaaaaaact taaatcccgg aacaaaaatg tttttgagta attttttaaa cagagaaaaa
    24601 gttctttttc ttgaaaattt aatagaactt ttaaaaaaaa taaatgaaaa agaagaagaa
    24661 ataaaaaatt taacaagaga aaagatgata gaaaagacag aagagataag aaaactcatt
    24721 tcagaaggag aaccacttga taattttata gtagatgcat ttgcgcttgt aagggaatct
    24781 gcaagaagga ctttaaatat gaggcatttt gatgttcaga tattaggagg gatagttttg
    24841 cattcaggaa aaattgcaga aatggctaca ggagaaggaa aaacactggt tgcaacttta
    24901 cctttatttt tgaatgctct tgaagggaag ggctgtcatc ttgtaactgt aaatgattat
    24961 cttgctaaaa gagatactca gtggatgggt ccaatttatc attatttagg tctttctgtt
    25021 ggatgtattg tttcatatag agaaaccaaa gatagatatt cttcagttgc ttacaaattt
    25081 gacccaacac accttcctcc tgattcaaga tttttatatt taaaacctgt tagtagaaaa
    25141 gaagcatacc tttgtgatat aacatatggg acaggcagtg aatttgggtt tgactattta
    25201 agagacaata tggcggtaag aaaagaagac caggtacaga gaaaattaaa ttatgcgata
    25261 atagacgaag ttgactctat tttaatagat gaagcaagaa ctcctttaat aatatccggt
    25321 ccaagtgaag agagtcccac tttgtattat gaagttgacc gacttgtaag gaaattgaca
    25381 aaggataaag attttgtagt tgatgaagaa aatcagaccg caactttaac agaagatggg
    25441 attaaaaaat gtgaaaaact tttaaatata ggaaatcttt atgatggaac tcatacagaa
    25501 cttgttcatc atataaatca agcattaaga gcacacaatt ttttcaagag agataaagaa
    25561 tatgttgtta aagatggaga agttattata gttgatgaat ttacaggcag attaatgcca
    25621 ggaagaagat ggtcagatgg acttcatcag gcaattgaag caaaggaagg ggtta
//
LOCUS       DAOSAT010000014        24168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_1508_length_24168_cov_5.680380, whole genome shotgun sequence.
ACCESSION   DAOSAT010000014 DAOSAT010000000
VERSION     DAOSAT010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 24168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24168
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1508_length_24168_cov_5.680380"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLF90_02140"
     CDS             complement(<1..1120)
                     /locus_tag="PLF90_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOK56117.1"
                     /translation="MIEKFISELKNKYKENIVKVLEVDEKEGSFYPLPDFILPVLKEK
                     LKEKGIERIYLHQLKALEKLNQGENIVVTTPTGSGKTLIYNIFIINEIYKNPDIKGLY
                     IFPTKALTQDQLKNLRGFDIKAEIYDGDTPDDLRRKIRSNPPNIILTNPDMLHTAFLP
                     FHEKWRSFFSKLKFIVIDEIHTYRGIFGSNVSHVIRRLRRICNLYGSNPQFILLSATI
                     NSPEKFAEELVGVKFVSISESSSPLPKKYIIFWNSQYESYYTQAIELLKKSIDYGLTT
                     VVFTNSRKSAELIQLWAVKKDRKIENIVSSYRAGYLPEERREIERKLFKGELKGIIST
                     SALELGIDVGYLDTCILFGYPGTIISTWQRIGRVGRKRE"
     gene            1295..2293
                     /locus_tag="PLF90_02145"
     CDS             1295..2293
                     /locus_tag="PLF90_02145"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOK56118.1"
                     /translation="MKRKIEFFKIFFILILSSSFLYAHLCDNVFRQADSLIVKPETYS
                     LVVKDKTTFKIFLQNNMDRGIAEISLIPESSAFDFEVTPKKMSIPKGRQVYFEVTIYP
                     KPEIKTGNYSIKFKLVGGGREFKSFTLGSTMEEKKETQIDISKLPNIKPIETPPVIDG
                     LFGDEAWKKATVLSNFTSTKGSEAIYKTWTLLTYDRNNLYIGIYCRDENLQNLTSDDK
                     VEIILSPSGNGSGYVLSFSPVGNPVYKKYDKTKQVSEWNPYGIKYAVNKVQNYWGIEI
                     SLPFSSMNISSPKEKEIWSIRITRFKTSGIQETSFWAMDITGYHSEKNLGKIVLNP"
     gene            2297..2836
                     /locus_tag="PLF90_02150"
     CDS             2297..2836
                     /locus_tag="PLF90_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56119.1"
                     /translation="MKKNLILPIFLLIYNICLSSEKLSIDFTYPSLKGDFYVNGKVSF
                     SPSSVISEDNITVNDFNSGEEIPTKITVLERWPDNSILEAEILFPANTNKQRKYVLTY
                     GIDVKRKNKFSQTSVLPIINASIGKTPVSTESINIDVGELLVKVDRSPEVRYYWYIIP
                     LLTLLFFTIFRTIKNSKKV"
     gene            2833..3855
                     /locus_tag="PLF90_02155"
     CDS             2833..3855
                     /locus_tag="PLF90_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK56120.1"
                     /translation="MRNKISFLRRVVQIVSLILIVYGGYIFKTRLEAETGKPLFPKLK
                     APEDAMSTTKFEKGKILWPSGTNPVLENYPPSLICRFNPRGGLFKACIFHFISENLTW
                     QTHIKYLLPHLTLFILLCFLFGRIWCGWVCPIGTVGDFITSIRKKMNIPAKRFPSGFR
                     VSLRLFSYGIMAIAFFISTLIGIPNFRRFQCYLFLPYCQICPARLICPLFGLIKPGWK
                     DFENAITSTFTILAWIVMGIFLAAFYFGRRIWCHLCPVGLINSWFNRGAGIELKKKAV
                     NCNKCGLCTETCPMGLTEMYDKNTDGIYNQNGCIMCLRCVEICPKDGCLSVSFFGKKI
                     TESKFK"
     gene            3868..5121
                     /locus_tag="PLF90_02160"
     CDS             3868..5121
                     /locus_tag="PLF90_02160"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOK56121.1"
                     /translation="MKKENKKFIDEKYCKILENLYDKKPEIFTDYKKEGEKYKRFSHL
                     KTINYFYNVIIDTEKLKKIKGKKPIVGYFCALVPEELIIASGGIPIRLCNEDLHCAES
                     GEEIIQGDICPLIKAICGSLSQNEINNFDLLVITGSCDGKVKLAEILSPVVKDIYFLD
                     IPRNSDYKESIEIWEKAYSDFYEYLKKKFKTKPKRNDLIEVCKMTNERTRIFRKIYDL
                     RGKKTGIMNSFDYFIMANASFFTSIEEWTDNAKKLYDEISKIEIKKNKYNKKILLAGP
                     PIIFPNFKILEILEEVGCYVACDTMCSAYGHLYNPVEIDEETEKSVIRTLTLKYIAPS
                     LCSCFIGIDKQINAILEFVEKYNLDGVVYYNLRLCNVFEIQIPVLRNILKEKGIPFLS
                     LKTDLSKEDTGQLKTRIEAFIEMIG"
     gene            5124..5888
                     /locus_tag="PLF90_02165"
     CDS             5124..5888
                     /locus_tag="PLF90_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOK56122.1"
                     /translation="MRIKAGIDIGSVTTKCVIVEDGIIKGKAITRTTAEPEKCAEIVL
                     NKAIEESGIKESDIESIVSTGYGRRIFRKANKIITEITGVAKGAYYLIGKKKCIVIDV
                     GGQDTKVVEVSENGEVVDFLMNDKCAAGTGRFLEMMANVFGIDVENFSNLAMNSKNPV
                     KINSTCSVFAESEVVSLITSGTPKEDIAGGLFNSIASRIAGMVRQFGQSELIVFCGGG
                     AKITALKYSIEKVIEKEIIVLPEPQFVVAFGAAITE"
     gene            complement(5938..7008)
                     /gene="ispG"
                     /locus_tag="PLF90_02170"
                     /gene_synonym="gcpE"
     CDS             complement(5938..7008)
                     /gene="ispG"
                     /locus_tag="PLF90_02170"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005898355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOK56123.1"
                     /translation="MKNTKIVKIGNICIGGKNPVAIQGMTKVPTSNLPVLKKQIKKMI
                     KEGAEIIRFSVLNEYDTDSIPVLKKEFQIPFVADIHYDWRLAKLSIEKGIDKIRINPG
                     NIEKKYLKEIIKVAKDYNIPIRIGLNSGSVKLKGDLVSSLIDSAKDIVKFFEDNNFFS
                     IVISLKTPFVKETIEAYRKISEIFDYPLHLGITEAGTGYLAISKSILGIGILLNEGIG
                     DTIRVSLTGPPEEEIKVAKSILQSLNLRIFEPEIISCPTCGRMKVNLKEVLERVKKEI
                     KKIEKKYPEIKELKIAVMGCSVNGPGEAKQADIGIAGGDKKWALFKKGKIIGTYKESK
                     IVEKLIEEITPDHPTFKVDSED"
     gene            complement(7005..8081)
                     /gene="rseP"
                     /locus_tag="PLF90_02175"
     CDS             complement(7005..8081)
                     /gene="rseP"
                     /locus_tag="PLF90_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOK56124.1"
                     /translation="MFSSIIALIILFSVLITIHEFGHFIVAKRNGVKVEKFAIGFGPK
                     IYQKKKGETTYIISVIPFGGYVKLAGEEIDKEKFEEWEYMGKSPGIRSKILFAGSLNN
                     LIFGFILLIPVFMIGTISYEGTKIGDFIKNFPAEKSGLKVGDEVLEVNGEKCKTWLDV
                     TLKIKDYTKKYKDKPLNLKIKRENEILIYSIKPAPYTVEENGKKLTEYIIGILPKEKL
                     EKYPFHRAVIKSGKEFFEISFTTLIGLKLLFQRKLSIRHFTGPVGISEIAVAAWKVGF
                     VSYLQFMAILSINLGIINLIPYPVLDGGHIFGLLIEKIRKKRPNMKTLQIIQNIGAIS
                     LILFALFITYQDIGRIFERNLKIK"
     gene            complement(8066..9208)
                     /gene="dxr"
                     /locus_tag="PLF90_02180"
     CDS             complement(8066..9208)
                     /gene="dxr"
                     /locus_tag="PLF90_02180"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOK56125.1"
                     /translation="MKNIVIFGSTGTIGQNTLELIRRQKEKFNILCIACKKNKKLFSK
                     QIEEFRPKYAYIEEKDEDFMRKYKNVKFFYGEEGLIEISNLKEGDLFIFAIPGIKTLP
                     AFIECIKNKKKVALATKEILVSGGEIIQDLKKVYNFEILPVDSEHNAIFQILKYENKG
                     IKKIYLTASGGPFYGKKKKNVKVKEALNHPVWKMGKKITIDSATMMNKTFEIIEAHYL
                     FSLREEQIDVVIHPEAIIHGMVEFIDGTLKLVGFIPDMKFPINYVLNYPERVDSKLKE
                     IDFGKINKLNFKKVNEEAKWLNFAKEAIRKKGSYPVVLNGANERAVEFFLDGKINFGN
                     IMNVIEKVVEKHQFKEKLDIKDIFYYHNWARNQVERIIGGDKCSVQ"
     gene            complement(9205..9912)
                     /locus_tag="PLF90_02185"
     CDS             complement(9205..9912)
                     /locus_tag="PLF90_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOK56126.1"
                     /translation="MEKLKIIPVHIGIIMDGNGRWAEKRNLPRIFGHKKGIEAVRRTV
                     KVASKYKVKYLTLYSFSTENWKRPKEEIEFLFSLMERNLRKEGENLHKNNVRVRFSGR
                     RWELPENLIEIMDYVEKLTEKNTGLNLIFAINYGGRGEIVDSLKKIIKNRHKIEEIDE
                     NLINKYLYLPDIPDPDLIIRTSGEKRISNFLLWQSAYSEFYFTTVLWPDFDEKEFLKA
                     LYDYQRRKRKFGGIIDK"
     gene            complement(9915..10631)
                     /gene="lptB"
                     /locus_tag="PLF90_02190"
     CDS             complement(9915..10631)
                     /gene="lptB"
                     /locus_tag="PLF90_02190"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOK56127.1"
                     /translation="MLEIKNLKKSFRNKVVLKGISMNVKRGEIVGLLGPNGAGKTTTF
                     EIIMGFLSPDSGSIFLDSKDITELPTWKRAREGISYLPQEISIFKKLTVEENCEIVLE
                     DFYKEREERDKIREECLKKLEISHLKDKVAGNLSGGQKRRLEIARSLMFSPKFFLLDE
                     PFSGIDPKTVSEIQDIVLSLKNENIGILLTDHNVREALKITDRAYLIYEGEILIEGTP
                     EEILNHPESRSVYFGEKFQL"
     gene            complement(10634..11653)
                     /gene="ruvB"
                     /locus_tag="PLF90_02195"
     CDS             complement(10634..11653)
                     /gene="ruvB"
                     /locus_tag="PLF90_02195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOK56128.1"
                     /translation="MNERMTSPEIRNEDLKYENTVRPRNFEEFIGQNKVKENLKVFIE
                     AAKNRKEVLDHILFYGPPGIGKTTLAYIVAGELGVNIKASSGPVIEKIGDLAGILTNL
                     EEGDVLFIDEIHRLPRNVEEFLYSAMEEFRIDIVIGEGPKARSVKIPLKPFTLCGATT
                     RIGLLTSPLRNRFGILHRLDYYSNEELKEIIKRSAKILNIRIEEEGAEEIAKRSRGTP
                     RVANRLLRRVRDYAEVKGEGIINKEIAVMSLNKMDVDEEGLDEMDKKILEVIITKFNG
                     GPVGVKSLSIAVGEEVSTIEEVYESFLVRKGFIKKTPQGRVATELAYKHLGLNKYQEK
                     FDSLL"
     gene            complement(11650..12231)
                     /gene="ruvA"
                     /locus_tag="PLF90_02200"
     CDS             complement(11650..12231)
                     /gene="ruvA"
                     /locus_tag="PLF90_02200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOK56129.1"
                     /translation="MLYFIKGKIFLKTPTYVVIENNGIGYFVEVSLQTSEKINEGEET
                     FLYIYQHFSEDKINLYGFIEKEERELFLLLITVPGIGPKVALRVLSGLSIEEIYQGII
                     TEDVSIFKNVPGVGKKTAERMIVELKQKIEKIPILVDNYKEKEILINAVEALTVLGYK
                     RKDSAVIVGEILKEKKGNITLEELIKEALRKLT"
     gene            complement(12209..12691)
                     /locus_tag="PLF90_02205"
     CDS             complement(12209..12691)
                     /locus_tag="PLF90_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOK56130.1"
                     /translation="MRVIGIDPGINKVGYGVIDENLKVIDSGVFIPSLKLKFENKIEF
                     LLENIETLIEKFLPEFISIEEIYVAKNARIALKIGIFIGGIIGLSISKDIKFLLIPPR
                     EIKQLITGNGGATKEQVKFMVENLTGYYSFQSFEASDAVATAISAIFKLKENVIFHKR
                     "
     gene            complement(12697..13446)
                     /locus_tag="PLF90_02210"
     CDS             complement(12697..13446)
                     /locus_tag="PLF90_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK56131.1"
                     /translation="MSGHSKWHSIKHKKMAMDARRGKIFTKIIRELMVAAKTGGANPD
                     TNPRLRMAIEKAKSVNMPNDNIQRAIKKGSGEEGSINYEQVTYEGYGPGGVAIFVEVL
                     TDNKNRSASEIRSIFSRHGGNLAGAGSVSWIFERKGLITVKKENIEEDKLMDIVIEGG
                     AEDMKTEKETYEITTSVENFENVKKSLEENNIPIENASITYIPKNTVHLEGKEAEHLL
                     KLLDELEESEDVQNVYANFDISDEILEQVSK"
     gene            complement(13460..13990)
                     /locus_tag="PLF90_02215"
     CDS             complement(13460..13990)
                     /locus_tag="PLF90_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOK56132.1"
                     /translation="MKKVYVDGVVLNILTSTPIVILKSENGKVLPIVVGIFEAQSILF
                     VLEKAKFPRPLTHDLMKIIIEKLKGKIQKLEIHSLKENVYYADLVIEVNGKIERIDCR
                     PSDGIAIALRFDVPIFASEDLLENPDIIKYYDSDDFIKSNKFDRPIDKKEAEEFRKII
                     ENLSAKEFWKRLKEEK"
     gene            complement(13993..16008)
                     /gene="fusA"
                     /locus_tag="PLF90_02220"
     CDS             complement(13993..16008)
                     /gene="fusA"
                     /locus_tag="PLF90_02220"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOK56133.1"
                     /translation="MGKVVTIGLFGSTKSGKTILGEAILYKTGMITRMGDINQGNTTF
                     DFDEEEKTRLISINLAVGYVKRDDSIIYILDVPGYIDFVGEQISGIEASDIAILNVSA
                     VEGIEVGTEKIWEMISKKNLPSIIFINKLDSPDLNYRKVIDEIFSFLGNKAVLVNYPV
                     FENGQFKGVKNIFSEDVSSGGEFSELFQKSMDTLAELDDAVMEKYLETGKLTKEEIAK
                     SLKNGIVERTVFPVLFGSGLNQIGIEELIDFIFNFIPSTDEMPPLKGKNQDGQEVLVE
                     RKDTQVFTGIIFKTIFDPFAGRLSYIKVLSGKFFSNSQFLNSTKGVKERVGQLFRMQG
                     RKQEPVEVVYPGEIVTAAKLNSQTFDTICDLNTNIIYEKPHIPEGAVSYSIAPKIKGT
                     EDKLGNAIGRISEEDLTIRIFRDEETGETIISGMGDLHIDITVNKFKNKFGVEVEKGI
                     PKIAYKETITATANAEGKFKRQTGGRGQYGHCFIKIEPLPRGTGFEFVDQIVGGAIPR
                     NFIPSVEKGVRDAMKKGVLANYPIVDIRVILYDGSYHVVDSSDIAFQIAGSMALQKAV
                     SEAKPVLLEPIVNVEIRVPSENVGDVIGTINSKRGKVLDMLSSGSFQIVKAQVPLAEM
                     SNYTNELRSLTSGKGTFSMEFSHYEEVPSHIAAKIIEQRKKEKEEKT"
     gene            complement(16100..16795)
                     /locus_tag="PLF90_02225"
     CDS             complement(16100..16795)
                     /locus_tag="PLF90_02225"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOK56134.1"
                     /translation="MENLKEKIILVLTGDEEEKILYEVLKNVKYIELRIDEFLRNFNE
                     SDIIEWIKKTRKLGGVNIIGTIRWYKEGGENSFYLSDKKRLEIFRAVSNYIDIIDVEI
                     KSKICDKVIEICKSKNKKIILSYHNFKKTPDINILKKIVKKGKKDGADMVKIATKVLT
                     EKHLFTLTELSYNYSKKIGLIVIPMATSLYKRLIPLFFGSSFTYVALNKKTAPSQPSY
                     LEISKFIDLIEIH"
     gene            complement(16820..17485)
                     /locus_tag="PLF90_02230"
     CDS             complement(16820..17485)
                     /locus_tag="PLF90_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HOK56135.1"
                     /translation="MIDLHTHTILSDGDLIPSELARRADEKGYKVIAFTDHVDFSNID
                     FVIPAIIRVCEKINLNFRIKAIPGVEITHVNPVDIPNIVKISRNLGAKIIIVHGETIV
                     EPVAKGTNKKALESDIDILAHPGLLSEDEAKIAAERKISIEISARKGHSFSNGHVSRL
                     WYEYEFPLILNTDAHSPDDLITDDFAKKIIVSAGIKVEDVNKVFHNSYILAKSKLDIG
                     FEI"
     gene            complement(17482..17841)
                     /locus_tag="PLF90_02235"
     CDS             complement(17482..17841)
                     /locus_tag="PLF90_02235"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOK56136.1"
                     /translation="MVEEEKELGKVRIDNEVLASIAGVAATSVPGVNKVITGLMGGLK
                     KIIRKKQDVGIKVILGEGEVSFELSIAVDYGTNIPEITYQVQKKIKEEVEKMSGLKVS
                     NVDVIVKEVKPPKKEIK"
     gene            complement(17835..19199)
                     /gene="accC"
                     /locus_tag="PLF90_02240"
     CDS             complement(17835..19199)
                     /gene="accC"
                     /locus_tag="PLF90_02240"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOK56137.1"
                     /translation="MIRKLFVANRGEIALRVIRACKELEIPVVIGYSDADRYSLPVFL
                     ADERICIGPSKPKESYLNIPSIISSIEITGADAVHPGYGFLSENIQFVEICNSARIIF
                     VGPTPENLRMIGDKSLAKKIAKKAKVPVIPGYEENDFKKALHHAKKIGFPIMIKASAG
                     GGGRGMRKVYTVEEFEKMWNTCKEEAKISFGEESLYIEKFIERPRHIEIQVLCDNKGN
                     IYVFPERDCSIQRRHQKLIEESPSPFADRGLRKKLQKYAKKIVKYIKYRNAGTIEFLV
                     DQNKNPYFIEMNTRIQVEHPVTECVTGIDLVKSQIKIADNSKLDFSHRDIKINYHAIE
                     CRINAEDPENNFMPSPGRIKKLVLPAGPGIRIDTHIYEGYFIPPYYDSLIMKLITYGR
                     TREEAIERMKRALDEIVIEGIKTTVPLYKKIISHPLFLSGRYYVGFVDKVVSESNQKE
                     VIKW"
     gene            complement(19196..19492)
                     /locus_tag="PLF90_02245"
     CDS             complement(19196..19492)
                     /locus_tag="PLF90_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56138.1"
                     /translation="MRRKEEFVVGCFYHIYNRGVDKRGVFLEDRDYERFMEYLYIFNN
                     DKPVEYGGQGSEKREKLVYILCFTLMPDHFHLIVSPFKKDGLVKFSLEEDGARE"
     gene            complement(19489..19953)
                     /gene="accB"
                     /locus_tag="PLF90_02250"
     CDS             complement(19489..19953)
                     /gene="accB"
                     /locus_tag="PLF90_02250"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOK56139.1"
                     /translation="MKPEELKVYADFMKEYGLEYLEVKKGDFHLILSKKGEEIRERKV
                     IEESPKVNIDKIVEESKHISQELENIVYVKSPLVGTFYRTPSPTSSPFVEQGQFVKKG
                     DVLCIVEAMKVMNEIKSEYDGVVKEILVENGKPVEYGQVLFVLEIGGLISEK"
     gene            complement(19950..20372)
                     /locus_tag="PLF90_02255"
     CDS             complement(19950..20372)
                     /locus_tag="PLF90_02255"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOK56140.1"
                     /translation="MVNKRLIGSYLKRAKVRREILNQFMLKKSYSDVIRESQEIIELL
                     EKAILLNLNVTPPKWHDVIDLIIKHSEKLKLDIKKDFKKIEKDCKWLRSQREISFYGD
                     MDFIPEDFYGKKDAEKAIKIVNELFRIIEKLSLNLEGK"
     gene            complement(20365..20811)
                     /locus_tag="PLF90_02260"
     CDS             complement(20365..20811)
                     /locus_tag="PLF90_02260"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOK56141.1"
                     /translation="MNEKQFSDFINELRKECLKYYKENLVSIVIFGSYAEDKYTPFSD
                     IDLLLILEKAGSNYKMYTEFFSILDNIETIKKLKEKNIFPLISPIIKSKKSLIVELPY
                     LWSASFKIIYDKNNYFKKFLKKLDEFKKHRIEYFEYPIPHYIMKDG"
     gene            complement(20820..21374)
                     /gene="efp"
                     /locus_tag="PLF90_02265"
     CDS             complement(20820..21374)
                     /gene="efp"
                     /locus_tag="PLF90_02265"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOK56142.1"
                     /translation="MNVLDLKEGNVFEYEGKLYIVKSTTHVVQQQRRGIVKIKAVEID
                     TGRGVEFSFRSDVEVKLVILEEVPLTFLYSDGSTYYFMDNNSYEQFNLGDEIIGNKKY
                     YLKENLEVTGLFYQGRIIDIQLPFTVDLKVIEAEPGYKGDTVQGGRKKVKVETGLIVQ
                     TPLFIEVGDTIRIDTRTGEYITRI"
     gene            complement(21443..>24168)
                     /locus_tag="PLF90_02270"
     CDS             complement(21443..>24168)
                     /locus_tag="PLF90_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56143.1"
                     /translation="DNKNKIFYGETTLNFILPPTDKDVVLFEVRMDTTNIAGFDDVEG
                     KGERYLYSSVLEFLPSYARQLNILVQPVQLFITGYTPKVPNASSVANALKNLIPAMLP
                     IDKKDLHIWTAPTTTFYGGVLSMFSTTALLNRIANSLALTQGFVNTTAAVGDWLTGKG
                     TAKIDFIVAVMPKGSMGEDVTGVNLPLRRGIIFVDESAPDAALHEMGHAIGLYTSKEQ
                     YDQFPPAGLPVEGLTAFVNDASSSITGFRGRVLHFPRETDTWYEEKYWYDIMGSSTTS
                     IWPIWGTLGAFGYSFRQILGDENKSAKSLVKGVPSGYKRIFVSAETIRYATIYYMKEG
                     SINAFEITDLATGKVDVPLQWSGNGRNDYTIKCFDKDGNEILFQDFTIVSPYPSYLGY
                     GSWLEESTWCGTFDIPENTMSIGIYPRPGWWNNFQSTPIFEVNATDYEPIKITGLLPG
                     QQLGESVEIKWTQDNTKQFSNLQYLVMYSVDGGSSWMPVGLPIEGNKITLPTDFLPSS
                     DNIVFKVIGSNGLGKVEAEVGGLKIENRQPKAIITTPKDGWVGDIGTKWILNGYGEDI
                     EDGIITEGIWEIDGESVNTKEVILNQGVHTITFTITDSGGLTGSESINVEVKEMENID
                     IGIEEGDFKLLIESKDPLNTSPVVYLKENGVHRAILKIQNTGKDTVFTASLYLKKPSG
                     TEELLKSANFTPGAFEEVVISETFIAEVEGEYRIRGEIKDITPEDTNSQNNERIWIYR
                     TKPNKPVIGVNPDILDFGSTKGRKEGYILVKNYGSDDLTVSSITITGINAVDFKVETE
                     FPITIPEDNLTLIPVYFVPNTKGIKEGKLKVGSNDNENPEVEIILKGECIGILGNING
                     DEQVDISDVILCLRMAILIDEPDPDAGDMNDDGVIDISDVILVLRKAIGLE"
BASE COUNT         7591 a         4405 c         2971 g         9201 t
ORIGIN      
        1 cttctctttt ccttcctact ctaccaattc tctgccatgt acttataatt gttccaggat
       61 agccaaaaag aatacaggta tcaagataac cgacatctat tccgagttca agagcacttg
      121 ttgaaattat tcctttcaat tctcccttaa ataatttcct ttcaatttct cttctttctt
      181 cgggtaagta tcctgctctg taagaagaaa ctatattttc aatttttcta tcctttttta
      241 ctgcccataa ttgaatgagt tctgctgatt ttctgctatt agtaaaaact actgtagtaa
      301 gtccataatc tatacttttt ttaagaagtt caattgcctg agtgtaatac gattcatatt
      361 gagaattcca aaaaataata tattttttag gtaaggggga tgaactttca gaaatactta
      421 caaacttaac tccaacaagt tcttctgcaa atttttcagg actattaatt gttgcagaaa
      481 gtaaaataaa ttgaggatta ctaccataaa gattacatat tcttctcaac cttcttataa
      541 catgggaaac attacttcca aaaattcctc tataagtatg aatttcatct ataacgataa
      601 attttaattt tgaaaaaaaa cttctccatt tttcatgaaa tggtaaaaaa gcagtatgaa
      661 gcatgtctgg atttgtcaaa attatgtttg gtggattact tcttattttt ctccttaaat
      721 catctggtgt atctccatcg taaatctctg cttttatatc aaatcctctt aaatttttta
      781 actggtcctg tgtaagtgct tttgttggaa aaatataaag acccttaata tccggatttt
      841 tgtaaatttc gtttataata aaaatattat aaataagtgt ctttccactg cctgtgggtg
      901 tcgttactac tatgttttct ccctgattta gtttttcaag tgccttcaat tgatgtaaat
      961 aaatcctctc aatacctttt tctttaagtt tttcttttaa aacaggcaga ataaaatcag
     1021 gcaaaggata aaaactacct tctttctcat caacttccaa taccttaaca atattttctt
     1081 tatatttatt ttttagttct gatataaatt tttcaatcat ttctcccata attacatttt
     1141 ataatacctt ttacataatg aatttagcaa atccctataa atccctttca aaatttgctt
     1201 ccatcaatca ttcctacttc ccacctcaaa ggtgggatgg aaaaagttga caccaaaaaa
     1261 ataaagggta ttattatatt aaaaggagta atatatgaaa aggaaaattg agttttttaa
     1321 aatttttttt attttgattt tatcatcttc gtttctctat gcgcatcttt gtgataatgt
     1381 ttttagacag gcagattctt taattgtaaa accggaaaca tattctctcg tagtaaaaga
     1441 taaaacaact ttcaaaatat ttcttcagaa taatatggat agaggaattg ctgaaataag
     1501 tttaattcct gaaagttctg cttttgattt tgaagtaact cctaaaaaaa tgtcaatacc
     1561 aaaaggaaga caggtctatt ttgaagttac aatttatcca aaacctgaaa taaaaacagg
     1621 taattactcc ataaaattta aacttgttgg aggaggaaga gaatttaagt catttactct
     1681 tggaagcact atggaagaaa aaaaagaaac acaaattgat ataagtaaac taccaaatat
     1741 taaaccaata gaaactcctc ctgtaattga tggtctattt ggagatgaag catggaaaaa
     1801 agcaacagtt ctttctaatt tcacttctac aaaaggttct gaagcaattt ataaaacatg
     1861 gacactttta acatatgaca gaaataatct ttatattgga atatactgca gggatgaaaa
     1921 tttacagaat ttaacttcgg atgataaagt tgaaataatt ctctctcctt ctggaaatgg
     1981 ctcaggatat gttttaagtt tttcacctgt tggaaatcct gtatataaaa agtacgataa
     2041 aacaaaacag gtttctgaat ggaatccgta tggaataaaa tatgctgtga ataaagttca
     2101 aaattactgg ggtattgaaa tatctttacc tttttcttca atgaatatta gttctccaaa
     2161 agaaaaagaa atatggtcta taagaattac aagatttaaa acaagtggaa tacaggaaac
     2221 aagtttctgg gcaatggata taacaggtta tcacagtgag aaaaatctcg ggaagatagt
     2281 cctgaatcct taaaaaatga aaaaaaacct tatactacct atatttttat taatatacaa
     2341 tatttgctta tcttctgaaa aattgtcaat tgattttaca tatccttcac ttaaaggaga
     2401 tttttatgta aatggaaaag tatctttttc tccttcatct gttatttctg aagacaatat
     2461 tacagtaaat gattttaaca gtggagaaga aattccaaca aaaataacag ttcttgaaag
     2521 atggcctgat aattctattc ttgaagcaga aattcttttt cctgcaaata caaataaaca
     2581 gaggaaatat gttttaactt atggaattga tgtaaaaaga aaaaataaat tcagtcagac
     2641 aagtgtttta ccaattataa atgcttcaat aggaaaaact cctgtttcaa cagagtcaat
     2701 caatatagat gttggagaat tacttgtaaa ggttgataga agtcctgaag taagatatta
     2761 ctggtatata attccccttt taactttatt gttttttaca atattcagaa caattaaaaa
     2821 ttcaaaaaaa gtatgagaaa caaaatttct tttttaagaa gggttgttca aattgtatca
     2881 ctgattttaa ttgtttatgg tggctatatt tttaaaacaa gattggaagc agaaacagga
     2941 aaaccattat tcccaaaatt aaaagcacct gaagatgcta tgtcaacaac aaagtttgaa
     3001 aaaggaaaaa tactctggcc atcaggaaca aaccctgttt tagaaaatta tccaccatct
     3061 cttatatgca gatttaaccc aagaggtgga ttatttaaag catgtatatt tcattttatt
     3121 tctgaaaatt taacatggca gacacatata aaatatttac tccctcattt aacactattt
     3181 attttactct gttttctttt tggaaggata tggtgtggat gggtatgtcc aataggaaca
     3241 gttggagatt ttataacttc aataaggaaa aaaatgaata tacctgcaaa aagatttcct
     3301 tcgggttttc gagtttcttt aagattattt tcttatggaa taatggctat tgcttttttt
     3361 atttctacat taataggaat accaaatttc agaagatttc aatgttatct ttttttgccg
     3421 tactgtcaaa tatgtcctgc ccgtcttata tgtcctttat tcggcttgat taaacctggt
     3481 tggaaagatt ttgaaaatgc tatcacttca acatttacaa ttcttgcatg gattgttatg
     3541 ggaatttttc ttgctgcatt ttattttgga agaagaatat ggtgtcatct atgccctgtt
     3601 ggtcttataa attcatggtt taacagagga gcaggaattg aacttaaaaa gaaagcggta
     3661 aattgtaata aatgtggact ctgtactgaa acatgcccga tgggacttac tgaaatgtac
     3721 gataaaaata cagatggtat ttataaccaa aatggatgta ttatgtgttt aagatgtgtt
     3781 gaaatatgtc caaaggatgg ttgtttatca gttagtttct tcgggaaaaa aattacagaa
     3841 tcaaaattta aataaaaagg atgataaatg aaaaaagaaa ataaaaaatt tattgatgaa
     3901 aaatactgta aaattcttga aaatctttat gacaaaaaac cggaaatttt tactgattat
     3961 aaaaaagaag gagaaaaata taagagattt tctcatttaa aaactattaa ttatttttac
     4021 aatgttataa ttgatactga aaaattgaaa aaaataaaag gaaaaaaacc tattgttgga
     4081 tatttttgtg cacttgttcc tgaggaatta ataattgcat ctggtggaat acctataaga
     4141 ttatgtaacg aggatttgca ctgtgctgaa tctggagaag aaataattca aggagatata
     4201 tgtcctttaa taaaagcaat ctgtggaagt ttatcacaaa atgaaataaa taattttgac
     4261 cttcttgtta ttacaggttc atgtgatggt aaagttaaac ttgctgaaat attaagtcct
     4321 gttgtaaagg atatttattt tcttgatata ccgagaaaca gtgattataa agaaagtatt
     4381 gaaatatggg aaaaagcata ttctgatttt tatgagtatt taaaaaagaa attcaaaaca
     4441 aaaccgaaaa gaaatgacct gatagaggtt tgcaaaatga caaacgaaag aacaagaatt
     4501 ttcagaaaaa tttatgattt aaggggtaaa aaaacaggta taatgaactc ttttgattat
     4561 ttcataatgg cgaatgcttc tttctttact tcaatagaag aatggacaga taatgccaaa
     4621 aaactttatg atgaaatatc aaaaattgaa ataaagaaaa ataaatataa caaaaaaatc
     4681 ctacttgcag ggcctccaat aattttccca aattttaaaa ttcttgaaat actggaagaa
     4741 gttggttgtt atgttgcctg tgatacaatg tgttctgcct acggtcatct ttacaatcct
     4801 gttgagatag atgaagaaac agaaaaaagt gttataagaa ctcttacact caaatatatt
     4861 gctccaagtt tatgctcctg ttttattgga attgacaaac agataaatgc aattttagaa
     4921 tttgttgaaa aatataatct tgatggagtt gtttattata atttaaggtt atgcaatgtt
     4981 tttgaaatac agatacctgt tttaagaaat atactaaaag aaaagggaat acctttttta
     5041 tcattaaaga cagatttaag taaagaggat acaggacagt taaaaacaag aatagaagca
     5101 tttatagaaa tgataggata gaaatgagga ttaaagcagg aatagatatc ggttcagtta
     5161 ctacaaaatg tgttattgtg gaagatggga ttataaaggg taaagcaata acgagaacaa
     5221 cagcagaacc tgaaaaatgt gctgaaatcg ttttaaataa agcaattgaa gaaagtggaa
     5281 taaaagaaag tgatattgag agtattgttt caactggata tggaagaaga attttcagaa
     5341 aagcaaataa aataataact gaaataacag gagttgcaaa aggtgcttat tatcttatcg
     5401 gaaagaaaaa atgtattgta attgatgttg gaggtcagga tacaaaagtc gttgaagtaa
     5461 gcgaaaatgg tgaagtcgtt gattttttga tgaatgataa atgtgctgct gggacaggaa
     5521 gatttcttga aatgatggca aatgtctttg gaattgatgt agaaaatttt tcaaatcttg
     5581 caatgaattc aaaaaatcct gtgaagataa attcaacctg tagtgttttt gctgaaagtg
     5641 aggttgtttc attgattact tcaggaacac cgaaagaaga tatagcaggt ggacttttta
     5701 attcaattgc ttcaagaatt gcaggaatgg taagacagtt tggacaaagt gaattaatag
     5761 ttttctgtgg gggtggagca aaaattacag cattaaaata ctcaatagaa aaagttattg
     5821 aaaaagaaat aatagttttg cctgaacctc aatttgttgt tgcttttggt gctgcaatta
     5881 cagaataaaa aatcccctct caaaacccgc ttgcctcaac catcttcact gtccacctta
     5941 atcttcactg tccaccttaa aggtgggatg gtcgggtgta atttcttcaa tgagtttttc
     6001 aactattttg ctttctttat atgtgcctat tattttgcct tttttgaata atgcccattt
     6061 tttatctcca cctgcaatcc ctatatctgc ctgctttgct tctccaggac cattcactga
     6121 acagcccatg actgctatct tcaattcttt tatttcagga tatttttttt caattttttt
     6181 aatctctttc tttactcttt caagaacttc ttttaaattt accttcattc ttccacaggt
     6241 aggacatgat attatttcag gttcaaatat tcttaaattt aatgactgta aaattgattt
     6301 tgcaactttt atttcttctt ctggtggacc tgttaaagaa actcttattg tatctcctat
     6361 cccttcattc aataaaattc caataccaag aattgatttt gatattgcaa gataaccagt
     6421 tcctgcctct gttattccaa gatgtaatgg ataatcaaaa atttccgata tttttctgta
     6481 tgcttctatt gtttctttta caaatggtgt ttttaaagaa atcacaattg aaaaaaaatt
     6541 attatcttca aaaaacttaa ctatatcttt tgctgaatca attaaagaag aaaccaaatc
     6601 tccttttaat ttaaccgaac ctgaattcaa acctattctt atcggaatat tataatcttt
     6661 cgctaccttt attatttctt tcaaatactt cttttcaata tttcctggat ttattcttat
     6721 tttatctatt cctttttcaa ttgataattt tgccaagcgc cagtcataat gaatatctgc
     6781 aacaaatggt atttggaatt ctttttttaa aacaggtatt gaatctgtat catattcatt
     6841 tagaaccgaa aacctaatta tctctgcacc ttcttttatc atttttttta tctgtttttt
     6901 taaaacagga agatttgagg taggaacttt tgtcattccc tgaattgcaa ctggattttt
     6961 tccacctatg cagatatttc ctattttaac aatctttgta tttttcattt aatctttaaa
     7021 tttctttcaa aaattcttcc aatatcctga tatgtgatga aaagagcaaa taaaattaat
     7081 gaaattgccc ctatattctg aattatctgt agtgtcttca tattaggtct tttctttctt
     7141 attttttcaa ttaaaagccc aaaaatatga cctccatcta aaactgggta tggtattaga
     7201 tttattattc caagatttat actcaaaata gccataaact gtaaataact tacaaatcca
     7261 accttccatg cagcaactgc tatttctgat attccaacag gtcctgtaaa atgtctgatt
     7321 gaaagttttc tttgaaaaag aagtttaagt ccaattaaag ttgtaaaaga tatttcaaaa
     7381 aattccttac cacttttaat tactgcacga tgaaatggat atttttccag tttttctttt
     7441 ggtagaatcc ctataatgta ttctgttaat ttcttcccgt tttcttcaac tgtataaggt
     7501 gctggtttta tactgtaaat caaaatttca ttttctcttt ttattttcaa atttaaaggt
     7561 ttatccttat atttttttgt ataatcctta attttgagag tcacatcaag ccatgtttta
     7621 catttttctc catttacttc cagtacctca tctccaactt ttaaaccact tttttctgct
     7681 ggaaaatttt taataaaatc acctattttt gttccttcat aacttattgt tcctatcata
     7741 aatacaggga ttaaaagtat aaatccaaat attaaattat ttaaagagcc agcaaataaa
     7801 attttgcttc ttatgccagg actttttccc atatattccc attcttcaaa tttttcttta
     7861 tctatttctt caccagcaag tttaacatat ccaccaaatg gaatgactga aataatatat
     7921 gttgtttctc ctttcttttt ttgataaatt tttggcccaa aacctatggc aaatttttca
     7981 acctttactc catttctttt tgcaactata aaatgtccaa attcatgaat tgttattaaa
     8041 acagaaaaaa gtataattag agcaattatt gaactgaaca tttgtctcct cctattattc
     8101 tttcaacctg atttcttgcc cagttatgat aataaaaaat atctttaata tcaagttttt
     8161 ctttaaattg gtgcttttca actaccttct ctattacatt cattatattt ccaaagttta
     8221 ttttaccatc taaaaaaaac tcaacagccc tttcattcgc tccatttaaa acaacaggat
     8281 atgaaccctt ttttcttatt gcttcctttg caaaatttaa ccattttgct tcttcattta
     8341 cttttttgaa atttagttta tttattttcc cgaagtcaat ttccttaagt tttgaatcca
     8401 ctctttctgg ataattcaaa acataattta tcggaaattt catatcaggt ataaaaccaa
     8461 caagttttaa agttccatca ataaattcaa ccattccatg aattattgct tctggatgga
     8521 taacaacatc tatctgttct tctcttaatg aaaataaata atgtgcctct ataatttcaa
     8581 atgtcttatt catcattgtt gctgaatcaa ttgttatctt tttgcccatt ttccacacag
     8641 gatggttgag tgcttctttt acttttacat ttttcttttt tttcccataa aatggaccac
     8701 ctgaagcagt gagataaatt ttttttatcc ctttgttttc atactttaaa atctgaaata
     8761 ttgcattatg ttcactatca acaggtaaaa tttcaaaatt atatactttt tttaaatcct
     8821 gtatgatttc acctcctgaa acaagtattt cttttgtcgc aagtgccact ttctttttgt
     8881 tttttataca ttcaataaaa gcgggaagtg tttttatacc cggaattgca aaaataaata
     8941 aatcaccttc ttttaaattt gatatttcaa taagcccttc ttctccataa aaaaacttaa
     9001 catttttata ttttctcatg aaatcctcat ctttttcttc aatgtaagca tatttcggtc
     9061 taaattcctc aatctgtttt gaaaataatt ttttattttt tttacaggca atacataaaa
     9121 tattaaattt ctctttctgt cttcttataa gttcaagagt attctgtcca attgttcctg
     9181 ttgagccaaa aattacaatg tttttcattt atcaataatt cctccaaatt ttctctttct
     9241 tctttgataa tcataaagtg cttttaaaaa ttctttttca tcaaaatcag gccataaaac
     9301 agttgtaaaa taaaattctg aatatgctga ctgccagagt aaaaaattgc ttattctttt
     9361 ttctccactt gttcttatta ttaaatcagg gtcaggaata tctggaagat agaggtattt
     9421 atttattaaa ttttcatcaa tttcctcaat cttatgtctg tttttaatta ttttctttaa
     9481 actatcaaca atctctcctc ttcctccata atttattgca aaaatcagat ttaaaccggt
     9541 atttttttct gtcagtttct caacataatc cataatttca ataagatttt cgggtagttc
     9601 ccatcttctt cctgaaaatc tgactctaac attattttta tgtaaatttt ctccttcttt
     9661 tcttaaattt cgctccataa gagaaaaaag gaattcaatt tcttcttttg gtcttttcca
     9721 gttttctgtt gagaaagaat aaagagttag atattttact ttatatttac ttgccacttt
     9781 tacagttctc cttaccgctt ctattccctt tttatgacca aaaattcttg gaaggtttct
     9841 tttttcagcc catcttccat taccatccat aataattcct atgtgaacag gaattatttt
     9901 taatttttcc atttttaaag ttgaaatttt tcaccaaaat atacacttct tgattcagga
     9961 tgatttaaaa tttcctcagg tgtcccttct atcaaaattt ctccctcata aattaaatat
    10021 gctctatcag ttatttttaa tgcttctcta acattatggt cagttaaaag gattcctata
    10081 ttttcatttt ttaaacttaa aacaatatcc tgtatttcac ttactgtttt cgggtcaatc
    10141 cctgaaaatg gttcatcaag aagaaaaaat ttaggagaaa acattaaact tcttgctatt
    10201 tcaagtcgtc tcttctgtcc accagaaaga tttccagcaa ctttatcttt aaggtgggaa
    10261 atttcaagtt ttttcaaaca ttcttctcta attttatctc tctcctctct ttccttataa
    10321 aaatcttcaa gaacaatttc acagttttct tctactgtta attttttaaa tattgaaatt
    10381 tcctgtggca aataagatat accttctctt gcccttttcc atgtaggaag ttctgttata
    10441 tctttactgt ctaaaaaaat acttcccgaa tcaggagaaa gaaaccccat aattatttca
    10501 aatgtagtag ttttccctgc gccatttgga cctaaaagtc caacaatttc tcctctttta
    10561 acattcattg atattccctt caaaacaact ttattcctga acgatttttt caaatttttt
    10621 atttcaagca tttttataaa agagaatcaa atttttcctg atatttattt aatccaagat
    10681 gtttataagc aagttctgtt gcaactctcc cctgtggtgt ctttttgata aaacccttcc
    10741 ttacaagaaa actttcataa acttcttcta ttgtacttac ttcttctcca actgctattg
    10801 ataaactttt aactccaaca ggaccaccgt tgaattttgt aattatgact tcaagaattt
    10861 tcttatccat ttcatcaaga ccttcttcgt caacatccat tttattcaaa gacataacag
    10921 caatttcttt atttattata ccctctccct tcacctcagc ataatctcta actcttctca
    10981 ataatctatt tgcaactctc ggtgttcccc ttgacctttt tgctatttct tccgctcctt
    11041 cttcttctat tcttatattt aatatttttg ctgacctttt tataatttct ttaagttctt
    11101 catttgaata ataatcaagg cgatgaagta ttccaaatct gtttcttaaa ggagaggtta
    11161 aaagtcctat tcttgtcgtt gctccacata atgtaaatgg ttttaagggt attttaacac
    11221 ttcttgcctt tggtccttct ccaataacta tgtctatcct gaattcctcc attgcactgt
    11281 aaagaaattc ctcaacattt ctcggtaatc tatgaatttc atcaataaat aaaacatccc
    11341 cttcttccag atttgtaaga attcccgcaa ggtctccaat tttttctatt acaggtccag
    11401 aagatgcttt tatatttact ccaagttccc ctgcaacaat atatgcaagt gttgttttac
    11461 caattccagg aggaccgtaa aacagaatat ggtcaagaac ttcttttctg ttttttgctg
    11521 cctcaataaa aacttttaaa ttttccttta ccttattctg accaataaac tcctcaaaat
    11581 ttctcggtct taccgtattt tcgtatttta agtcctcatt tcttatttca ggagaagtca
    11641 ttctttcgtt catgttaatt tccttaatgc ttcctttatc aattcttcaa gtgtaatatt
    11701 acctttcttt tcttttaata tttctcccac aatcactgct gaatccttcc ttttgtatcc
    11761 aagaactgta agtgcttcca ctgcattaat cagaatttct ttttctttat aattatcaac
    11821 caaaattgga attttttcaa ttttttgttt taattctact atcattcttt ctgctgtctt
    11881 cttccccaca cccggaacat ttttgaaaat agaaacatcc tctgtaataa taccctgata
    11941 aatttcctca attgaaagtc cggatagaac tctcaatgca acttttggac ctattcctgg
    12001 aacagtaatt agtaataaaa aaagttccct ttcttctttc tctataaatc catacaaatt
    12061 tatcttatcc tctgaaaagt gctgatatat gtataaaaac gtttcttcac cttcatttat
    12121 tttttccgat gtctgaagtg atacttcaac aaaatagcca attccattat tttcaattac
    12181 cacataagtt ggtgttttta aaaaaatttt accttttatg aaatataaca ttttctttca
    12241 atttgaaaat tgcagatata gcagtagcaa ctgcatctga tgcttcaaaa ctttgaaaac
    12301 tgtaataacc tgttaaattt tccaccataa attttacctg ttcctttgtc gctcctccat
    12361 ttcctgtaat aagttgcttg atttctcttg gtggaataag aagaaattta atatcttttg
    12421 agatagataa accaataata cctcctataa aaatacctat ttttaaagca attctggcgt
    12481 ttttagcaac atatatttct tcaatagaga taaattcagg taaaaatttt tcaataagag
    12541 tttcaatatt ttctaaaaga aactcaattt tattttcaaa tttaagtttt aaagaaggaa
    12601 taaaaactcc actatcaatt acttttaaat tttcgtcaat tactccataa ccgactttat
    12661 taattcccgg gtcaatacca attactctca atttctttat tttgaaacct gttcaagtat
    12721 ctcatcagat atatcaaaat ttgcataaac attctggaca tcttctgatt cttcaagttc
    12781 atcaagaagt tttaaaagat gttctgcttc ttttccttca agatgaactg tattttttgg
    12841 aatataggtt atacttgcat tttctatcgg tatgttattc tcctcaagag attttttaac
    12901 attttcaaaa ttttcaactg aagtggttat ttcatatgtt tctttctctg ttttcatatc
    12961 ttctgctcca ccttcaataa caatatccat tagtttatct tcttctatat tttctttttt
    13021 aacagttatt aaaccttttc tttcaaaaat ccaggaaaca ctacctgcac cagcaagatt
    13081 tccaccatgt cttgaaaata ttgaccttat ttctgaagca cttctgtttt tgttgtcagt
    13141 taaaacttct acaaatattg ctacgcctcc tggaccgtat ccctcatatg taacctgttc
    13201 ataattaatt gaaccttctt ctccggaacc ctttttaatt gctctctgta tattatcatt
    13261 tggcatatta acactttttg ccttttctat tgccattctt aatcttggat ttgtatcagg
    13321 atttgcacca cctgtttttg ctgcaaccat aagttctctt ataatcttgg taaaaatctt
    13381 ccctcttctt gcatccattg ccattttttt gtgtttaata ctgtgccatt tagaatgtcc
    13441 tgacatatct gcctcctttt tatttttctt cttttaatct tttccaaaat tcttttgcac
    13501 tcaagttttc aattatcttt ctaaattctt ctgcttcttt tttatctatt ggtctatcaa
    13561 acttattaga tttaataaag tcatcgctat cataatattt tataatatcc gggttttcaa
    13621 gtaaatcttc tgaagcaaaa ataggaacat caaatcttaa tgcaattgca attccatcac
    13681 tgggtctaca atcaatcctt tcaatctttc catttacttc aatcacaagg tctgcataat
    13741 aaacattttc cttaagagaa tgaatttcaa gtttttgaat tttaccttta agtttttcta
    13801 taataatttt catcaaatcg tgtgtaagcg gccttggaaa ctttgctttt tcaagaacaa
    13861 ataaaattga ctgtgcttca aaaattccaa caactattgg cagtactttc ccattttcac
    13921 tcttcaaaat aactatcgga gtgctggtca aaatatttaa aacaactcca tcaacatata
    13981 ccttcttcat atttatgttt tttcctcttt ttctttcttc ctctgttcaa taatttttgc
    14041 cgctatatgt gaaggaactt cttcataatg agaaaattcc atagagaaag ttccttttcc
    14101 actggtaaga gaacgtagtt cgtttgtata atttgacatt tctgcaagag gaacctgtgc
    14161 ttttacaatt tgaaaactgc cagatgaaag catatcaaga acctttcctc tttttgaatt
    14221 tatagttcct ataacatccc ctacattttc agaaggtact cttatttcaa cattaactat
    14281 tggctcaagc aaaactggct ttgcttcact gactgctttt tgtaaagcca ttgaaccagc
    14341 tatctgaaag gcaatatctg aagaatcaac aacatgataa ctaccatcgt aaagaataac
    14401 tcttatatca actattggat aatttgcaag tactcccttt ttcattgcat cccttacacc
    14461 tttttcaaca gaaggtatga aatttcttgg gattgcacct ccaactatct ggtcaacaaa
    14521 ttcaaaacca gtacctcttg gaagtggttc tatttttata aaacagtgtc catactgtcc
    14581 tcttcctcct gtctgtctct taaatttccc ttctgcattt gctgttgctg ttattgtttc
    14641 tttataggca atttttggaa tacccttttc cacttcaaca ccgaatttgt ttttaaattt
    14701 atttaccgta atatctatat gtaaatctcc catccctgaa ataattgttt ctcctgtttc
    14761 ttcatctctg aaaatcctta tggtcaaatc ttcttcagaa attcttccta tcgcattacc
    14821 aagtttatcc tctgttcctt ttatttttgg tgcaattgaa tatgatacag caccttcagg
    14881 aatatgtggt ttttcataaa tgatgtttgt atttaaatca cagattgtat caaatgtttg
    14941 tgaattgagt tttgctgcgg tcactatttc tcctggatac acaacttcaa cgggttcctg
    15001 ttttctaccc tgcattctga ataattgccc aaccctttcc tttacacctt ttgttgagtt
    15061 taaaaactgt gaattagaaa aaaacttacc acttaaaact tttatataag aaagacgtcc
    15121 tgcaaaaggg tcaaaaattg ttttaaatat tatacctgta aatacctgtg tatcttttct
    15181 ttccaccaat acttcctgac catcctgatt tttacctttc aaaggtggca tttcatcagt
    15241 tgaagggata aaattaaaaa taaaatcaat caattcttct ataccaatct gatttaaccc
    15301 tgaaccaaat aaaacaggga aaacagtcct ttcaactata ccattcttta atgattttgc
    15361 aatttcttct tttgtaagtt ttcctgtttc aagatatttt tccattaccg catcatcaag
    15421 ttcagcaaga gtatccattg atttttgaaa taattcactg aattcacccc cagaagatac
    15481 atcttcactg aaaatatttt taactccttt aaattgtcca ttctcaaaaa caggataatt
    15541 cacaagtact gctttatttc ccagaaaaga aaaaatttca tcaataactt ttctgtaatt
    15601 taaatcagga ctatccagtt tattaatgaa aataatagat ggaagatttt ttttacttat
    15661 catctcccag attttttcag ttcctacctc tattccttct accgctgaaa catttaaaat
    15721 tgcaatatca gatgcttcaa ttccggaaat ctgttctcca acgaaatcaa tatatcctgg
    15781 aacatctaaa atgtatataa tactatcatc tctttttaca tatccaacag ctaagtttat
    15841 acttatcaac cttgtttttt cctcttcatc aaaatcaaaa gtagtatttc cctgattaat
    15901 atctcccatt cttgttatca tacctgtttt ataaagtatt gcctctccaa gaattgtttt
    15961 tcccgatttt gtacttccaa ataaaccaat tgtaacaacc tttcccattt tctactcctt
    16021 tcacatttct gtaaataaaa tttaatattc tgctaaaata ttataccaca tttaaaaaaa
    16081 tcagtaaaaa cccaagtagt taatgaattt caattaaatc aataaatttt gaaatttcaa
    16141 gatatgatgg ttgagaaggc gctgtttttt tatttaaagc aacataagta aaagaagaac
    16201 caaaaaaaag tggtattaat cttttgtata aagaagtggc catgggaata acgattaaac
    16261 caatcttttt tgagtaatta tacgaaagtt ctgtaagtgt aaagagatgt ttttctgtta
    16321 atacttttgt agcaattttt accatatctg ctccatcttt tttgcctttc ttaacaattt
    16381 tttttaatat gttaatatca ggtgtctttt taaaattatg gtaggaaagg ataatttttt
    16441 tgtttttact tttacagatt tcaataactt tatcacatat tttactttta atctcaacat
    16501 caattatatc aatataatta ctaactgctc taaaaatttc aagccgtttt ttatcagata
    16561 aataaaaaga attctctccc ccttctttat accatcttat agtacctata atgtttactc
    16621 cccctagttt ccttgttttt tttatccatt caattatatc actttcattg aagtttctta
    16681 aaaattcatc aattctcaat tcaatatatt tcacattttt taaaacttca tataaaatct
    16741 tttcctcttc atcacctgta agtacaagta taattttttc ttttaaattt tccatttccc
    16801 agtccctgtt ttactgaaat tatatctcaa atcctatatc aagttttgat tttgccagaa
    16861 tataactgtt atgaaatact ttgtttacat cttcaacttt tattcctgct gaaacaataa
    16921 ttttttttgc aaaatcgtct gtaattaaat cgtcagggga atgtgcatca gtattcaaaa
    16981 ttaaaggaaa ttcatattca taccataaac gagatacatg cccatttgaa aaagaatgtc
    17041 cttttcttgc agatatttct atactaattt ttctttcagc agcgattttt gcctcatctt
    17101 cacttaacaa tccaggatga gcaagaatat caatatcgct ttccagtgct tttttatttg
    17161 ttccttttgc aaccggctca acaattgttt ctccatgaac aataattatt ttagcaccga
    17221 gattccttga aattttaaca atatttggaa tatcaactgg attaacatgg gttatctcaa
    17281 caccaggaat tgcctttatt ctgaaattta aattaatttt ttcacaaact cttataattg
    17341 ctggaataac aaaatcaata ttggaaaaat caacatggtc tgtaaaagca ataactttat
    17401 atcctttttc atctgccctt cttgcaagtt cactcggaat taaatcacca tcacttaaaa
    17461 tcgtgtgagt atgaaggtct atcattttat ctccttttta gggggcttta cttctttaac
    17521 tattacatca acatttgaaa cctttaaacc actcattttt tcaacttctt cttttatttt
    17581 cttctgaacc tgataagtta tttccgggat atttgttcca taatcaacag caatgctgag
    17641 ttcaaaacta acttctcctt ctccaagtat tacctttatt cctacatcct gttttttcct
    17701 gattattttt ttcaatcctc ccattaaccc agttataact ttattaactc ctggaacact
    17761 ggtagcggca acacctgcaa tagaagcaag aacttcgtta tctattctta ctttccccag
    17821 ttccttttcc tcttctacca tttaattacc tccttttgat tactttcaga aacaacctta
    17881 tcaacaaaac ctacataata tcttcctgaa aggaataaag gatgggaaat aattttttta
    17941 tacagaggga ctgtggtttt tattccttct ataacaattt catcaagcgc tcttttcatt
    18001 ctttcaattg cttcttctct tgttcttcca taggttatga gtttcataat taaactatca
    18061 taatagggtg gtataaaata cccttcataa atgtgtgtgt ctattcttat tccaggtcct
    18121 gccggtaata caagtttttt tattctaccg ggagatggca tgaaattatt ttcagggtct
    18181 tcagcgttga ttctgcattc aattgcatga taatttattt ttatgtctct atgggaaaaa
    18241 tcaagttttg aattatctgc aattttaatc tggctcttaa caaggtctat tcctgtaaca
    18301 cactcagtaa ctggatgctc aacctgaatt cttgtattca tttctataaa atatggattt
    18361 ttattctggt caacaagaaa ttctattgtt cccgcatttc tgtattttat atattttact
    18421 atttttttag catatttctg caattttttt cttaaacctc tatctgcaaa tggtgatgga
    18481 ctttcttcta tcaatttctg gtgtcttctc tgaatagaac aatccctttc agggaaaaca
    18541 taaatatttc ctttattatc acacaatacc tgaatttcaa tatgtctcgg cctttcaata
    18601 aatttttcaa tatataaaga ttcctcccca aaagatattt ttgcttcttc cttacaggta
    18661 ttccacatct tttcaaattc ttcaactgta tatacctttc tcattcctct acctccacca
    18721 cctgcagatg cttttatcat tatcggaaac cctatttttt tagcatgatg aagtgccttt
    18781 ttgaaatcat tttcttcata accaggaata acaggaactt ttgctttttt ggctattttt
    18841 tttgcaaggg atttatcacc tatcattctt aaattttcag gtgttggacc gacaaaaatt
    18901 attcttgcag aattacaaat ttcaacaaac tgtatatttt cagaaagaaa accatatcct
    18961 ggatgaacag catccgcgcc tgttatttca attgaactta taatagaagg aatatttaga
    19021 taactttctt taggttttga aggtcctata cagattcttt catctgcaag aaaaacaggt
    19081 aaagaatacc tgtcagcatc agaataacca ataacaacag gaatttccag ttctttgcat
    19141 gctcttataa ctcttaaagc aatttctccc ctatttgcta caaacaattt tcttatcatt
    19201 cccgagctcc gtcctcctct aaagaaaatt ttacaagacc atctttttta aatggactta
    19261 caattaaatg aaaatgatca ggcatcaacg taaaacataa aatatacact aacttttccc
    19321 ttttttccga gccctgtcct ccgtattcaa ccggtttatc gttattaaaa atgtacaagt
    19381 attccataaa tctttcataa tccctatctt caagaaatac acctctttta tccactcccc
    19441 tattataaat atgataaaaa cagcctacca caaattcctc tttccttctc atttttccga
    19501 gatcagtcct ccgatttcaa ggacgaaaag gacctgtccg tattcaacgg gttttccatt
    19561 ctccacaagt atttccttca ccactccatc atattctgac ttaatctcat tcataacttt
    19621 cattgcttca actatacaca aaacatctcc tttttttacg aattgacctt gttcaacaaa
    19681 aggagaactg gtaggagaag gagtcctgta aaaagttcca acaagaggag atttaacata
    19741 aacaatattt tcaagttcct gagatatatg ttttgattct tctacaattt tatctatatt
    19801 cactttggga ctttcttcta taaccttcct ttctcttatt tcttcccctt ttttgctcaa
    19861 aatcaaatga aaatcgcctt tttttacttc aagatattct agaccatact ctttcataaa
    19921 atcagcgtaa acttttaatt cttcgggctt cattttccct ccagattcaa acttaatttt
    19981 tctataattc tgaacaattc attaactatt tttattgctt tctctgcatc tttttttcca
    20041 taaaaatcct caggtataaa atccatatct ccataaaaag aaatttctcg ctgacttctt
    20101 agccatttac aatccttttc aattttttta aaatcttttt taatatctaa ttttaacttt
    20161 tctgaatgtt ttattatcaa atctattaca tcatgccatt taggtggagt tacattcaaa
    20221 ttaagtaata ttgctttttc caacaattca attatttcct gagattctct tattacatct
    20281 gaataacttt ttttcagcat aaattgattt aatatttctc ttcttacttt tgctcttttt
    20341 aaataacttc ctattaatct tttgttaacc atctttcatt atataatgtg gtataggata
    20401 ttcaaaatat tcaatcctat gttttttaaa ttcatccagt ttttttaaga attttttgaa
    20461 ataattattt ttatcataaa taattttaaa acttgcagac caaagataag gcaattccac
    20521 tattaaactt tttttactct ttataattgg agaaattaat ggaaatatat ttttttcttt
    20581 aagtttttta attgtttcaa tattatcaag aattgaaaaa aattcagtat acattttata
    20641 atttgaacct gccttttcaa gaatcagaag caaatcaata tctgaaaaag gagtatactt
    20701 atcttctgca taggaaccaa aaatcacaat tgaaacaaga ttttccttat agtactttaa
    20761 acattccttt cttaactcat taataaaatc agaaaattgt ttttcattca tattttcaat
    20821 cagatgcggg ttatatattc acctgttctt gtatctatcc ttatggtatc tccaacttca
    20881 ataaataaag gcgtttgaac tataagacct gtttctactt tgactttttt tctcccccct
    20941 tgaactgtat ctcctttata tcctggttct gcctctatta ctttaaggtc aactgtaaaa
    21001 ggaagttgaa tgtcaattat tctaccctga tagaaaagac cggtaacttc aagattttct
    21061 ttcaagtagt attttttatt acctattatt tcatcaccca aattaaactg ttcataagag
    21121 ttattatcca taaaataata tgttgaacca tctgagtaaa gaaatgtaag aggtacttct
    21181 tctaaaataa caagtttaac ttctacatcg cttctgaaac taaattcaac acctcttcct
    21241 gtatctatct caactgcctt aatctttaca ataccccttc tctgctgctg aacaacatgg
    21301 gtagtacttt taacaatata taatttccct tcatactcaa aaacatttcc ttctttcaaa
    21361 tcaagtacat tcatactaaa aattatacca tacaaataaa aataaataaa cccccttctt
    21421 ccgaacccgg ttcggaaggt ggctattcaa gtcctatcgc ttttctcaaa acaagtatca
    21481 catctgaaat gtctatcact ccatcatcat tcatatcccc tgcatccggg tctggttcat
    21541 ctatcaatat tgccatcctc aaacataaaa tcacatcact tatatctact tgctcatctc
    21601 catttatatt tcctaaaatt cctatacatt ctccttttaa gattatctct acctctggat
    21661 tctcattatc atttgaaccc actttcaatt tcccttcttt tatccctttt gtatttggaa
    21721 caaaatatac aggtattaat gttaagttat cttctggtat tgttatcgga aattctgtct
    21781 ctactttgaa atctactgca tttatccctg ttattgttat tgaactaact gttaaatcat
    21841 cactcccata gtttttcaca agtatatatc cctctttcct tccttttgtg cttccaaaat
    21901 caagtatatc tggatttact cctatcactg gtttattcgg ttttgtcctg tatatccata
    21961 tcctctcgtt gttctggcta tttgtatcct ctggtgttat atcttttatc tctcctctta
    22021 ttctatattc tccttctact tctgctataa atgtctcact tattactact tcttcaaatg
    22081 ctcctggtgt aaaattcgca cttttaagta attcttctgt tcctgatggt tttttaaggt
    22141 aaagagatgc tgtaaatact gtatctttcc ctgtattctg tatctttaat attgctctgt
    22201 gtactccatt ctcttttaaa tatactaccg gtgatgtatt caatgggtct ttactctcta
    22261 ttagtaattt aaaatctcct tcttcaatcc ctatatctat attctccatc tcttttactt
    22321 ctacatttat actctcactt cctgttaaac caccactatc tgttattgta aatgttattg
    22381 tgtgaactcc ttgatttaat ataacttctt ttgtatttac actctctcca tctatctccc
    22441 atatcccttc tgttattatc ccatcttcta tatcctctcc atatccattt aatatccatt
    22501 ttgttcctat atctcctacc catccatcct ttggtgttgt tattatcgct tttggttgtc
    22561 tattttctat cttcagtcct cctacctctg cctctacttt tcccagccca ttacttccta
    22621 ttactttgaa tactatgttg tctgatgatg gtaaaaagtc agttggtaag gttattttgt
    22681 ttccttctat cggtaatccc acaggcatcc aactactccc tccatctacg ctatacatca
    22741 ctaaatactg gagatttgaa aactgctttg tattatcctg tgtccatttt atctcaacac
    22801 tttctccaag ttgctgtcct ggtaaaagtc ctgtgatttt tattggctca taatcagttg
    22861 cattaacttc aaaaattggt gtactctgaa aattgttcca ccagccaggc ctgggatata
    22921 ttcctattga catcgtattt tcaggtatgt caaaagttcc acaccatgtg ctttcttcta
    22981 accaacttcc atatcctaaa taactcggat atggagaaac tattgtaaag tcctggaaaa
    23041 gtatttcatt tccatcttta tcaaaacatt ttattgtata atcatttctt ccattcccgc
    23101 tccattgaag aggaacatct accttacctg ttgccaaatc tgtaatttca aaagcattga
    23161 tactgccttc tttcatgtaa taaatagtgg cgtagcgtat tgtttccgct gaaacaaaaa
    23221 ttcttttata gccagaagga actcctttca ccaaagactt tgcactttta ttttcatctc
    23281 caaggatttg acgaaaagag tatccgaatg cgccaagagt tccccatatt ggccatattg
    23341 aagttgtaga agaacccatg atgtcatacc agtacttttc ttcgtaccag gtatctgtct
    23401 cccttgggaa atgcaagacc ctgcccctga aacctgttat tgaagaactg gcatcattaa
    23461 caaaagcagt cagtccctca actggaagtc ctgcaggtgg gaactggtca tattgttctt
    23521 tacttgtgta caaacctatg gcatgaccca tttcatgtag tgcagcatca ggggcacttt
    23581 catcaacaaa tattattcct cttctcaacg gcagattcac tcctgttaca tcttctccca
    23641 tacttccttt cggcatcaca gccacaatga aatcaatttt tgctgttcct tttcctgtaa
    23701 gccagtcgcc aacggctgct gttgtattta caaaaccctg tgtcaaggca agagaattgg
    23761 ctattctatt aagtaaagca gtcgtactga acattgacaa tactccacca taaaaagtcg
    23821 tagtaggtgc tgtccagata tgaaggtctt ttttatcaat cggcaacatt gcaggtatta
    23881 agtttttaag tgcatttgca acagatgaag catttggtac ttttggggta taacctgtaa
    23941 taaataattg aactggttga acaagtatat ttaactgcct tgcataggaa ggtaaaaatt
    24001 caagtacact tgaatataaa tacctttctc ctttcccttc tacatcatca aatccagcaa
    24061 tatttgttgt atccatccta acttcaaaca ataccacatc tttatcagtc ggtggaagaa
    24121 taaaatttaa tgttgtctcg ccatagaaaa ttttattttt attgtctg
//
LOCUS       DAOSAT010000015        22938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_1614_length_22938_cov_5.068697, whole genome shotgun sequence.
ACCESSION   DAOSAT010000015 DAOSAT010000000
VERSION     DAOSAT010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22938
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1614_length_22938_cov_5.068697"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..879)
                     /locus_tag="PLF90_02275"
     CDS             complement(19..879)
                     /locus_tag="PLF90_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOK56144.1"
                     /translation="MTGIDFIVLVIFGSGVLTGILRGFIGGIIDIVGIISGVSLASIA
                     YKAPVNLFRRFNIIGTPVDILFFHLFSFLFILSVIVGIEVLRKKIYVKHIVDRIFGFF
                     TGIIEGMFFAGFLLNIMSASPNAAKEIDHSKFPKYITRFIPGIYLKNDRMGITLPKLI
                     ALPKNYQDEFDREKLSITFQKVNFYKMKGTCIKCGGEVEFSGYFLKAGTSVIPKFTCK
                     ICGRTSDGCQTFEIFHFLYNECPVELARKGMKFDCGNFPNRELVMPKTECPVDKNQLQ
                     IWEWQPPVKY"
     gene            complement(956..1327)
                     /locus_tag="PLF90_02280"
     CDS             complement(956..1327)
                     /locus_tag="PLF90_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56145.1"
                     /translation="MKRFIIPMLISVFFIGLLYNGYSQERREKQGGDQFQRERREGPL
                     GEAFEIQRKIKEIEIQTIQNDPELKKMQEQISSLQKQLNEKLQQKLSTNQEYQELKKK
                     QEEMRKQWEERRREREGEKKQ"
     gene            complement(1349..1744)
                     /locus_tag="PLF90_02285"
     CDS             complement(1349..1744)
                     /locus_tag="PLF90_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56146.1"
                     /translation="MKKLFVLLLSINLIISFTLNAQNRKPDLSKSKEKIEKLLKENFI
                     LKEKLKEFLDTQKRIKEIEREEIEKDPELKNLHIQIMNLRIQLMQNLQEKLKDNEEYQ
                     RLKKRKEEIYEKMKKEKEERKKEIEERKN"
     gene            1826..2485
                     /locus_tag="PLF90_02290"
     CDS             1826..2485
                     /locus_tag="PLF90_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOK56147.1"
                     /translation="MGYKKKIAIFDLDGTLIDAYDSIYKTIDFICEKINCEKFDYERV
                     KRAVGGGDLKLICNLFDKDKIEVAHKLYREYYLNFLKGNVKLMRGCIEILSQLKEKKL
                     KIAVATNRSNFCLDFLLKESGIKNFFDIILCADDVENPKPHPEIILKILEKTGFKKEE
                     TFYVGDMDIDYETGKNAGVDTFIVLTGSCRKEDFLEYKNPLIFEDLIQLKKFLIENIL
                     I"
     gene            2528..3793
                     /gene="eno"
                     /locus_tag="PLF90_02295"
     CDS             2528..3793
                     /gene="eno"
                     /locus_tag="PLF90_02295"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOK56148.1"
                     /translation="MEILSVYGRQILDSRGNPTVEVEVMLEDGTTGRAAVPSGASTGE
                     REALELRDGDKEFLGKGVKNAIKNVNEIIGPEIEGESVFDQRGIDYKMIELDGTENKS
                     RLGANAILGVSLAVSRAAANVLDVPLYFYIGGVYANVMPVPMCNVINGGLHADNNLDI
                     QEFMIAPIGAKSFSDGYRMVAEVFQYLKKILKDKGYVTSVGDEGGFAPNLNKNEEAIQ
                     LIIQAIEKAGYTPGEEIYIALDTAASSLYEEGLYQFEGKKISSDELIDFYEKIVSKYP
                     VYSIEDGLAENDWEGWKKLTEKLGNKIQLVGDDIFVTNPKIFKEGIRQKIANAILIKV
                     NQIGTLTETLETIEMAKRAGYRTIISHRSGETEDTFIADLTVGTNAGQIKSGSLSRSE
                     RLCKYNQLLRIEENFGNTCKYYGEIIKEK"
     gene            3790..4053
                     /locus_tag="PLF90_02300"
     CDS             3790..4053
                     /locus_tag="PLF90_02300"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOK56149.1"
                     /translation="MRKIRKNWFLIFTVFFTICCGIRLKTLISSYQTYRFYKNEIANL
                     ERENKNLQEKIEKIKNDPFYIEKILREEYGMIKEGELIIKTGE"
     gene            4054..5325
                     /gene="serS"
                     /locus_tag="PLF90_02305"
     CDS             4054..5325
                     /gene="serS"
                     /locus_tag="PLF90_02305"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOK56150.1"
                     /translation="MYPFKFVRENIELLKKVAELKNQKVDWERFEELDNERRRLIMFV
                     EERKKWQKDLTEKISRSKEDKDAVSFLINQAKKYSDEIKETEKRLSEIEKEIEKIISF
                     IPNIPHPSVPVGKSEKDNVIVREWGEIKEKKFNVLPHWEIGEKLGILDFPRGAKITGS
                     FFPVFKGKGALLVRALINFMIDLHTKNGFKEVWVPSLVNRDSLFNTGQLPKLEDDMYY
                     LERDDFFLIPTAEVPVTNLHKDEVLKEEDLPIYYVAYTPCFRREAGAYGKETKGLIRV
                     HQFDKVELVKFVKPENSYDELESLLNEAEKVLQALNLPYRVVLLCTGELSFAGSKCYD
                     IEVWCPGVGKYLEVSSCSNFESFQARRANIKYRKKDGKTDYVHTLNGSGVALPRTIIG
                     IIENYQTENGEVIIPEVLRDYMKMDRLTLNE"
     gene            5318..6346
                     /locus_tag="PLF90_02310"
     CDS             5318..6346
                     /locus_tag="PLF90_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOK56151.1"
                     /translation="MNEALRNNLLTIIYFAIIIVICLIVKWFWEYFLIRVSKGRELDL
                     KILKRTGKGVVFFVFFSGLSILWERTSLLIKTENLPLQKLLLNLPKIIYSFIVLSVTF
                     IISGIVDGFVEWYLEKVAERTKTKVDEEFMSLFKRVSRIIIFFIGITIILNKFNQPIT
                     SILGAAGIASFAIAFAAQDTLSNMISGFFIIIDKPFKIGDRIKLATGEIGEVIEIGMK
                     RTKILSPENNVIIISNSEIAKTRIVNFGFPDSKIKIRLKVGIQNLKDKEKIKDLFMEI
                     VKKNNFFEKDTSEVYLTEFATSQAVFLLSFNILDYKKELNVVDKIIEEIKNKFEEEKI
                     ELLFVRRE"
     gene            6350..7504
                     /locus_tag="PLF90_02315"
     CDS             6350..7504
                     /locus_tag="PLF90_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOK56152.1"
                     /translation="MIKEVDNVVERIKADIINWRRDFHMYPELGFEEYRTSEKIKEVL
                     KKTGIKYEVKGKTGVIGYINKNFSKTIGIRADMDALPIEEQTGLEFSSKNKGIMHACG
                     HDGHVATLLGVGIILNKFKDFLKYNVKLIFQPCEEKLPGGACELIKEGVLENTDFLIG
                     FHFISDIPLNKIYIEKGIVMANTDRFRIIIKGKGGHGSAPDSTNDPVVCSAFLISSLQ
                     TIVSRRISPFQPCVVSVCKIKGGEAFNVIPDKVEIGGTVRTFSEKTRKKIKNEMKNIC
                     EKIVSSFKCKMDFSYESYSPAVKNDPDFTEIIKKTTKEILSTKFFYKYHPSMGGEDFA
                     FYSKKVPSTYIFIGSGKKSGLHHSSKFNLDERVLPFTVKYLSNLILKLKI"
     gene            7515..8165
                     /locus_tag="PLF90_02320"
     CDS             7515..8165
                     /locus_tag="PLF90_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOK56153.1"
                     /translation="MKKVIFVWGGWEGHQPKETTEIFAEIMKREGYDVEVYNTLDIFL
                     DKQKMGSVNLIVITWTMGKITSEQTKGLLDAVSSGVGIAGWHGGMGDAFRENTGYQFM
                     VGGQWVAHPGGIVDYEINIVKKDDPIVKGISDFKLKSEQYYMHVDPSNEVLATTTFRN
                     IEFPWIDGTVMPAVWKRIYGKGRVFYASFGHQVSDFEVPEAREIVKRGLIWAVGDL"
     gene            8220..8873
                     /gene="pal"
                     /locus_tag="PLF90_02325"
     CDS             8220..8873
                     /gene="pal"
                     /locus_tag="PLF90_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOK56154.1"
                     /translation="MKFIKFIIAGLIAVSFFGCMCPTKIKKEFLEEKEKEEKPELTSI
                     LKPEEKIEPKPAEETKPTKPDDGKATPEEVKTAIKEGEIPVTPIPPEAKFIKPEEMDS
                     ELKEIFRNIYFDFDKYSIREDAAQILKKIGDYLLKNPDIKILIEGHCDERGTREYNLV
                     LGEQRALSARRFLVIMGVSPKRLYTVSYGEDMPADPGHNEEAWAKNRRCEFKIEVKR"
     gene            8870..9781
                     /locus_tag="PLF90_02330"
     CDS             8870..9781
                     /locus_tag="PLF90_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOK56155.1"
                     /translation="MRKKILFPILSLLLIISFFTGCSDIATKDDIDAVKEKISNLEDD
                     FYATVKQISEKFNNIENDYKQKDDNIKKNIDEVSDRILVLSNEMNSIKDEINVMKGKI
                     DESNFENSEKIKKLNNEFIEKNLEIKREIEWIKKDIENVKLLYNDFSLKISTLTQNLS
                     ETQTNIKTDIISLRDSQVKIVETLQNLPKKIDQIEEKINVVDKKFNEVVNSFLDEFAR
                     HESEIYQLKKQISENEKLSERNTSASTSSIVKSILKTGKEKYHIVQKGEYLTLIAKKY
                     NTSVSEIKKLNNLKSDVVYPGQKLLIP"
     gene            9860..11743
                     /locus_tag="PLF90_02335"
     CDS             9860..11743
                     /locus_tag="PLF90_02335"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013466483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOK56156.1"
                     /translation="MEENYIKIFKKTLREEEFEKLKKIKSKKVLNFIGESILLCEPDD
                     VFICSDTPDEIAHIKNMAIVSKEESAALLTPGHTFHFDGYYDQGRDREVTKFLVPEGD
                     KLSSALNQIEREKGLNEVKNLLKGSMKGKTMIVRFLILGPANSVFTIPCVECTDSWYV
                     AHSVDLLYRHGYKEICKIDENTDIFFTLHSAGELDENMTSKNYDKKRIYIDYTENTVY
                     SVNTQYAGNSVGFKKLALRLAIRKAHKEGWLAEHFMIIGVYGPGGRKTYIAGVFPSAC
                     GKTSTAMLEGEKILSDDLAYVRNIDGICKAVNVECGIFGIIQGVNPVDDPLIYRTITS
                     PNEIIFSNILVKDGKTWWLDMGCELPEEGINYSGKWYKGKKDEKGEEILPSHKNARYT
                     ISLRALSNCDEELENPEGVELGGIIFGGRDYRGYVPVQQSFNWAHGIIAYGVALETET
                     TFAIVGQEGKYEINIMSIQDFVSIPLGQYIKNYLLFGEKLDKKPLVFGVNYFLRSLKT
                     GDFLNSRKDKHVWVKWMELRIHNDVKAIETPTGFIPIYEDLKILFNDVLKKEYSKEDY
                     IEQFTIRVLENLMKIERVLKFCREKIEEAPDEVFEVLNQQKERLIKAREKFGDYISPE
                     RFI"
     gene            11764..12912
                     /locus_tag="PLF90_02340"
     CDS             11764..12912
                     /locus_tag="PLF90_02340"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOK56157.1"
                     /translation="MELLSERAKLLKPSATMALNKKAKEMKAKGLDIVNLTVGEPDFD
                     TPEEIKEYAIKAMKEGYTKYTEEKGLKELREAICEKLEKENKLKYEPEEIVVSNGAKH
                     SLFNIFLSILNPGDEIIIPVPYWVSYPAMVSICGGVPVFCNFNEKFKIDIDDLKKKIT
                     KKTKGIILNSPSNPTGIVYDKKELEEIAEIIISNKILCISDEVYEKIIFDSEYFSIAS
                     LSDEIKELTVVVNGVSKTFAMTGWRIGYTASKRYLADAFGKIQGQTTSAPSTISQKAS
                     IYAIKEGEKLYRNMIEEFKKRKNFLVENLSEEIKFPYPEGAFYMFLNFKNMDSKDFAE
                     KLLEEKLVATVPGCEFGVDNYIRISYATSMENLKKAVERINEFVKENK"
     gene            12909..13715
                     /locus_tag="PLF90_02345"
     CDS             12909..13715
                     /locus_tag="PLF90_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56158.1"
                     /translation="MRKISILIFLFCPFFLFSQEYKFKQSKPLNYTFQMKGDVNLNYP
                     GTNEKFNVFAKGNLKIETLGIEEEVYILKITPFKTIVKVDEQVLEDITNIETGISSVI
                     STSLVKMKNNGEIIEMAETNKGILSLSQVLKLLPVFSENLSSGKKWKQTIPAFNFPGI
                     PMCALEFNYSYEKKGNSGLIHLTGIQTIKETRKDKDTKITFDGKNSSKGSFVFNEDEG
                     EISNFNGNFLLDLNTKFEIPPSPDVKEKKIETLTIKIKLNLNITLSKLTS"
     gene            13831..14826
                     /locus_tag="PLF90_02350"
     CDS             13831..14826
                     /locus_tag="PLF90_02350"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOK56159.1"
                     /translation="MIYKNVELYNVEEIREDNDGLQLLRIPETLRSKLNDNAKNASLY
                     PSGCEIRFNLKSEICKIVLKGINGNGICEVFFGDFFESWRLIKGEPTEIEIRYPQNIE
                     YLSKLSKEKNLKFDPKLVRVILPHLLITKIVDIGGEFELPRKNQTPKVKYLAYGSSIT
                     HGATSLRPTGTYASKIAQILNVDLINLGFGGGAHLEKEIADYIAERDDWDFGTFELGI
                     NMVGGFEVDEFEKRVDYFIEKIVDKKPDKYLFFIDIFPFYMDFDNQEKQEKFREIVKN
                     KVKEVNKDNVIHISGFDILKSLKGLTFDILHPSPFGMEEMAYNLANIMKSYGLVD"
     gene            14840..15841
                     /locus_tag="PLF90_02355"
     CDS             14840..15841
                     /locus_tag="PLF90_02355"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOK56160.1"
                     /translation="MLYRNIEFYNVEEIFERENGLQLSRIPERVRVKLNDNAKTRAIM
                     PAGCELRFRLKSQKAKIVMKTDTEVVWRNGICEIYFGDFFERWYFVSENPSIIEIQYP
                     QNLEKLKELKKEKGLKFDPEVVRIILPHLVDLRILEIEGEFNPPEKNQVPDKKFIAYG
                     SSITHGGFSIVPSYTYVSKTAYILGMDAINLGLGGGAHLEKEIADYIAERDDWEFGVF
                     ELGVNMVGGFEVDQFEKRVDYFVEKIVKENPEKYLFFIDIFPFYMDFDNQEKQKKFRE
                     IVKNKVKQIKKKNVIHISGFKILKDVRGLMTDRVHPSSQGFEEMGYNLASILKKYVV"
     gene            15878..17236
                     /locus_tag="PLF90_02360"
     CDS             15878..17236
                     /locus_tag="PLF90_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOK56161.1"
                     /translation="MRKYLKELLIISFPLIISSSIWTIQHFVDRMFISWYSVKALAAV
                     LPASFLNLTTTSIFVGTASFVNTFVAQYYGAETYEKTGEIVWQGLYLALIGGILHFLF
                     IPLTPFMFSIFAHEKEVLSYEVIYFQILCVGAFSVIASSSLSSFFSGRGKTNIVLFVS
                     FLQTTLNLIFDYLLIFGKWIFPEMGIKGAGIATVISGYFSFFVYLLLIFQKKNLMYGI
                     KNFRFNKVLFKNLLKYGVPSGMHFFLDISVWTIFLLIIGKLGVIPLSSSNIAFNINTV
                     AFMPMIGIGTGVSILVGQYIGKGKPEIGEKITYIGFLITFIYMCSIALTYIIFPDFYI
                     KFFRPRVYTGDFEQVRNLTVILLRFVAFYSIFDTMNVIFLSGLRGAGDTKFIMNVFLF
                     ATIFIFLIPLYICTFIFNSGIFIGWSIATLYVIVIGFSFFIRFLKGKWKKLKVIETPV
                     LL"
     gene            17319..17657
                     /locus_tag="PLF90_02365"
     CDS             17319..17657
                     /locus_tag="PLF90_02365"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOK56162.1"
                     /translation="MEDREKMVSFLAYFFGWISGLIIFLTEKKSEYIRFNAMQSIIFS
                     GVLTVIYIFISIVSLIPFIGKILSLILHPLVYLAGTAIWIVLLIKSFKGEYFKLPVIG
                     DYAEKYSKTL"
     gene            17740..18366
                     /locus_tag="PLF90_02370"
     CDS             17740..18366
                     /locus_tag="PLF90_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOK56163.1"
                     /translation="MEEGSLFHVFTKSIAGYKVFKSKVDYRRMVETIWYYMDERTAKF
                     SRVIDLKKENKGEPVVRIICYCIMPTHLHLVLEEKKEKGISQFMSNILNSYARYFNLK
                     YKRKGHLWESSYKRVLVETDEQLLHLTRYIHLNPTTAGIVKKPENWEFSSYREYLGNE
                     KIFLKICYFDDIIEINPKSYREFVEDYIDYEKELHRIKGLVKFESENP"
     gene            18417..19451
                     /locus_tag="PLF90_02375"
     CDS             18417..19451
                     /locus_tag="PLF90_02375"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOK56164.1"
                     /translation="MKVAYLVGKKKIVIEEEKNPEIEREDDVLVRVRCVGICGSDVHY
                     FLEGRIGDQIVKDKIIVGHEASGEVIEVGKNVKKLKKGDKVAIEPGISCGKCEFCIKG
                     KPNICPNVKFLGTPPVNGAFREYMVMPEENLIKIPDGLEYEEGVLSEPLAIGVYTVKL
                     SQIETGDDVAILGAGPIGLSILFSARISGANRIFVSDLIKERLEFAEKIGANYTVDAK
                     KNDITEIVKKITNNRGVDISFEAAGKRETFRQVIHTSRIGGKCVFVGIPTEDTVEFEA
                     HIMRRKELKLINIRRSAFCTEIALNLMKNSELPFKEMITHRFPFEKIEEALNLVAEYR
                     DGVIKCVVNI"
     gene            19569..20426
                     /gene="panC"
                     /locus_tag="PLF90_02380"
     CDS             19569..20426
                     /gene="panC"
                     /locus_tag="PLF90_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOK56165.1"
                     /translation="MEVVKKIEQVRKIVGKAKKEGKVIGFVPTMGYLHKGHISLIRRA
                     KKECDFVAVSIFVNPTQFGPNEDFDRYPRDFERDRKILEEEKVDLVFFPEVEEMYPYD
                     FKTWVYVEKYSEILEGKFRPGHFKGVCTVVIKLLNIVQPDKSYFGWKDAQQLIIVKKM
                     VEDLNLPCEIIGCETVREDDGLAESSRNVYLNEEERERATCLYRALKRIEEMVKKEKI
                     YDCKKLIEEGRKIIEKENVEIQYLEIVRISDLMPVEKIEKDVIVLGAVKIGNVRLIDN
                     IRLNGFENL"
     gene            20725..21258
                     /locus_tag="PLF90_02385"
     CDS             20725..21258
                     /locus_tag="PLF90_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOK56166.1"
                     /translation="MQEIIWYYRYERKNKFSKVKDFMSEDFGDKLVVIVSYCIMPTHL
                     HFTLGEEKEGGISKFMANILNSYTRYFNLKHNRKGPLWESRFKYVLIETQEQLLHLTR
                     YHHLNPVVAELVKKPENWKFSSYREYLNYDGGLKICEFEELIDMSSSEYKRFVEDNID
                     YQKEIHKIKELIKLETL"
     gene            21307..22317
                     /locus_tag="PLF90_02390"
     CDS             21307..22317
                     /locus_tag="PLF90_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56167.1"
                     /translation="MELTKELYEAIITIVEDKVKDIKVTREEFELLRKAVLDLTEAQR
                     KAEERLTRLEEAVERLAEAQKKAEERLVRLEEAVERLAEAQRKAEERLVRLEEAQIRT
                     EERLVRLEEAVERLAEAQRKTEERVSRLEQVVERLEQAVEKLAEAQRKTEERVGRLEE
                     AVEKLAEAQRKTEERVGRLEEAMEKLAEAQRRTEEKLGKLEETQRNLQIEIAKLSDTI
                     GFGIEDVAKVVLPGYLERHYKIKIGEIERKFFRIENKEIEVNLYAEGKKGSKKIYLVG
                     EAKSRIYERDVEKFISENMLFIEKFGEKNIIKVLFGFYIHPSASEIAKKNKILLVASY
                     MR"
     gene            22693..>22938
                     /locus_tag="PLF90_02395"
     CDS             22693..>22938
                     /locus_tag="PLF90_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56168.1"
                     /translation="MLRIIDHEGGSDQVNITIDNTPPDSDIRIFGTVRVGSTISYTGS
                     WSDNLSGPVIVKEAWLYGDTDTDTSNAVKIKDLTISET"
BASE COUNT         8603 a         2535 c         4546 g         7254 t
ORIGIN      
        1 atatcttaga gaaatttctt aatattttac aggtggttgc cattcccaga tttgaagttg
       61 atttttatcc actggacatt ctgtttttgg catgaccagt tctctatttg gaaaattacc
      121 acagtcaaat ttcattcctt ttcttgcaag ttcaacagga cattcattgt acagaaaatg
      181 aaagatttca aatgtctgac atccatcaga tgttcttcca caaattttac atgtaaattt
      241 aggtattaca cttgttcctg cttttaaaaa gtatcccgaa aattcaacct cacctccaca
      301 ctttatacat gttcctttca ttttataaaa atttactttt tgaaaggtaa ttgacagttt
      361 ttctctatca aactcatcct ggtaattttt tggcaaagca attaattttg gtaaagttat
      421 tcccattctg tcatttttta aataaattcc aggaataaat cttgttatgt attttggaaa
      481 ttttgagtgg tctatttctt tggcagcatt tggtgaagca ctcataatat ttaaaagaaa
      541 gccagcaaaa aacatacctt caattattcc tgtaaaaaat ccgaaaattc tatcaactat
      601 atgttttaca tagatttttt ttctcagaac ttcaatcccg actattaccg aaaggataaa
      661 caaaaatgaa aataaatgaa aaaaaagtat atcaacagga gttcctataa tattaaatct
      721 cctgaataaa ttaacaggtg ctttatatgc aattgaagca agagaaactc cacttataat
      781 tccaacaata tctataattc caccaataaa acctcttaaa attcctgtta agacaccgct
      841 tccaaaaata accaaaacaa taaaatcaat tccagtcata agtaaataat agcataaaat
      901 aaaaaaaaat aaaaagggac tacctctact taaaaggtag tccctttaaa ataaattatt
      961 gcttcttttc tccctctctt tctcttcttc tttcctccca ctgttttctc atctcttcct
     1021 gttttttctt caattcctga tattcctgat ttgttgaaag tttctgctga agtttttcat
     1081 tcagttgttt ctgaagagaa ctaatctgtt cttgcatttt tttcaattca gggtcattct
     1141 ggattgtctg gatttctatt tctttaatct ttctctgaat ttcaaatgct tctcccaaag
     1201 gtccttctct tctttctctc tggaattgat ctccaccctg tttttctctt ctttcctgtg
     1261 agtatccatt atataaaagc cctatgaaga aaacacttat aagcatagga ataataaatc
     1321 ttttcatatt ttttcacctc ctttcgtttc aattttttct ttcttctatt tctttttttc
     1381 tttcttcctt ttcttttttc attttttcat aaatttcttc ctttcttttt ttcaatctct
     1441 gatattcttc attgtccttt agtttctcct gaagattttg catcaattga attcttaagt
     1501 tcattatctg tatatgtaaa tttttaagtt cagggtcttt ttctatttct tctctttcaa
     1561 tttcttttat tcttttctgt gtgtcaagaa attctttaag tttttctttt aaaatgaaat
     1621 tttccttcaa taatttttca attttttctt ttgatttaga taaatctggt tttctattct
     1681 gtgcattgag agtaaatgaa attataagat ttattgaaag aagtagtaca aaaagtttct
     1741 tcattttttt cctccttttt tattttagac atttgaaaat aaaaaaggtt tagtatatta
     1801 tagtaaaact tttttagagg aaaaaatggg ttataaaaag aaaattgcaa tttttgacct
     1861 tgatggtact ttgatagacg catatgattc aatttataaa acaatagatt ttatttgtga
     1921 aaaaatcaat tgtgaaaaat ttgattatga aagagtgaaa agagcagtcg gtggaggaga
     1981 tttgaaatta atatgtaatc tttttgataa ggataaaatt gaagttgctc ataaacttta
     2041 cagagaatat tatttaaatt ttttgaaggg taatgttaaa ttaatgaggg gctgtattga
     2101 aattttaagc caacttaaag aaaaaaaatt aaaaattgct gttgctacaa acaggtcaaa
     2161 tttctgttta gattttttat tgaaagaaag tggaattaaa aatttttttg atattatact
     2221 atgtgctgat gatgttgaaa atccaaaacc gcatcctgaa ataattttga aaattctgga
     2281 aaagacaggt tttaaaaagg aagaaacttt ttatgttgga gatatggata ttgattatga
     2341 aacagggaaa aatgcaggtg ttgatacatt tatagttctc accggttcct gcagaaaaga
     2401 agattttttg gaatataaaa atcctttaat ttttgaagac ctgatacaac ttaaaaaatt
     2461 tttaattgaa aacattttga tttgagaata taatactaaa ataatgaaat gaaaatagga
     2521 ggtaaaaatg gaaatattaa gtgtttatgg aagacagatt cttgattcaa ggggaaatcc
     2581 aacagtagag gtagaagtta tgcttgaaga cggaacaaca ggaagagcag cagtcccttc
     2641 aggggcttca acaggagaaa gagaagcact tgaattgaga gatggagata aagaatttct
     2701 cgggaaagga gtaaaaaatg cgataaaaaa tgtaaatgaa attataggtc ctgaaattga
     2761 aggtgaaagt gtttttgacc agagaggtat tgattataaa atgattgaac ttgatggaac
     2821 tgaaaataaa tcccgtttag gagcaaatgc tattcttggt gtgagtttgg ctgtttcccg
     2881 cgctgctgca aatgttctgg atgtcccatt atatttctat attggaggag tttatgcaaa
     2941 tgtaatgcct gtgccaatgt gtaatgttat aaatggtgga ttacatgcag acaataacct
     3001 tgacattcag gaatttatga tagcaccaat tggagcaaaa agttttagtg atggatatag
     3061 aatggttgca gaggtattcc agtatttaaa aaagatatta aaggataagg gatatgtaac
     3121 ttcagttggt gatgaaggag gttttgctcc aaatttaaat aaaaatgaag aagcaataca
     3181 acttataata caggcaattg aaaaagcagg ttatacaccg ggagaagaga tttatattgc
     3241 tcttgatact gctgcttctt ctttatatga ggaaggcctt tatcaatttg aagggaaaaa
     3301 aataagttct gatgagttaa ttgatttcta tgaaaaaatt gtatcaaaat atcctgttta
     3361 ttctattgaa gatggacttg cagaaaatga ctgggaagga tggaaaaaat tgacagaaaa
     3421 actcggaaat aaaattcaac ttgttggaga tgatatattt gtcacaaatc ctaaaatttt
     3481 caaagagggg attagacaga aaatagcaaa tgcaatttta ataaaagtta atcagatagg
     3541 aacactcaca gagacacttg agacaattga aatggcaaag agggcaggat acagaacaat
     3601 aatatctcac aggtctggtg aaacagagga tacttttata gcagacctta cagttggaac
     3661 aaatgcaggg cagattaaaa gtggttcatt gagcaggtct gaaagattgt gtaaatataa
     3721 tcaacttttg agaatagaag agaattttgg aaatacatgc aaatattacg gtgaaataat
     3781 taaagaaaaa tgaggaaaat aaggaagaac tggtttttaa tttttacagt tttttttaca
     3841 atctgctgtg gaataaggtt aaaaacatta atttcttcat atcaaacata cagattttat
     3901 aaaaatgaaa ttgcaaacct tgaaagagaa aataaaaacc ttcaggagaa gatagaaaaa
     3961 ataaaaaatg acccctttta tattgaaaag atattgagag aggaatacgg aatgataaaa
     4021 gaaggtgaac ttataataaa aacaggagaa taaatgtatc cttttaaatt tgtaagagaa
     4081 aatatagaac tgttaaaaaa agtggcagaa ttgaaaaacc agaaagttga ctgggaaaga
     4141 tttgaagaac ttgataatga gagaagaagg ttgataatgt ttgttgagga aagaaagaag
     4201 tggcagaagg acttaacaga aaaaatttca agaagtaaag aagataaaga tgcagtttct
     4261 tttttgataa atcaggcaaa aaaatattct gatgaaataa aggaaacaga aaaaagatta
     4321 agtgaaattg aaaaagaaat tgaaaaaata atctctttta tcccaaatat tccacatcct
     4381 tctgttcctg ttggaaaaag tgaaaaagat aatgttattg tgagagaatg gggagaaata
     4441 aaggagaaga aatttaatgt tcttccccac tgggaaatag gtgaaaaact tggtatttta
     4501 gattttccaa gaggtgcaaa aattacagga agtttctttc ctgtctttaa aggcaaaggt
     4561 gctcttcttg tcagggcatt gataaatttt atgattgatt tgcatacaaa aaacggtttt
     4621 aaagaggtct gggttccttc tcttgttaac agagatagtt tattcaatac aggacaattg
     4681 ccaaaacttg aagatgatat gtattacctt gaaagagatg atttcttttt aatcccgaca
     4741 gcagaagttc ctgtgacaaa tctgcataag gatgaggttt taaaagagga agatttgccg
     4801 atttattatg ttgcatatac cccatgtttc agaagagaag caggagcata tggaaaagaa
     4861 acaaaaggtc ttataagggt tcatcaattt gacaaagttg aacttgttaa atttgtaaag
     4921 ccagagaatt cttatgatga acttgaatca cttttaaatg aagcggaaaa agttcttcag
     4981 gcattaaatc ttccttacag ggttgttctt ttatgtactg gggagttgag ttttgctgga
     5041 agtaaatgtt atgatattga agtatggtgt ccaggtgttg gaaaatatct tgaagtttct
     5101 tcttgttcta attttgaaag ttttcaggca agaagggcaa atattaagta cagaaagaaa
     5161 gatggtaaaa cagattatgt tcacactctt aatggttcag gagttgcttt accgagaacc
     5221 ataataggaa taattgaaaa ttatcagaca gaaaatggag aagtgattat ccctgaagtt
     5281 ttaagagatt atatgaaaat ggataggtta actctaaatg aatgaagcat taagaaacaa
     5341 tcttctaaca attatttact ttgcaataat aatcgtaatc tgtcttattg ttaagtggtt
     5401 ctgggaatat tttttgataa gagtttcaaa gggaagagaa cttgatttaa aaattttgaa
     5461 aaggacagga aagggggttg tcttttttgt atttttttct ggattaagta ttttatggga
     5521 aagaacatcc cttcttataa aaacagaaaa tttacctctt caaaaacttc ttttgaatct
     5581 tccaaaaatt atctattctt ttattgtcct ttcagttact tttataataa gcggaatagt
     5641 tgatggattt gttgagtggt atcttgaaaa ggttgcagag agaacaaaaa caaaagtgga
     5701 tgaagaattt atgtcattat tcaaaagggt ttcaagaata ataatatttt ttataggcat
     5761 tacaataatt ctgaataaat tcaatcagcc aataacaagt attcttggag cggcaggaat
     5821 tgcctctttt gctattgctt ttgctgctca ggatacactt tcaaatatga tttcaggttt
     5881 ttttattata attgacaaac cctttaaaat aggagataga attaaacttg caacaggaga
     5941 gattggagaa gttatagaaa ttggaatgaa gaggacaaaa attttatcac ctgaaaataa
     6001 tgtaattatt atttctaatt ctgaaatagc aaaaacaaga attgttaatt ttggatttcc
     6061 agattcaaaa attaaaataa gattgaaagt aggtattcag aatttaaaag ataaggaaaa
     6121 gataaaagat ttatttatgg aaatagttaa gaaaaataat ttttttgaaa aagatactag
     6181 cgaggtttat cttactgaat ttgcgacttc tcaagcagtt tttctgttaa gtttcaatat
     6241 tcttgattat aaaaaagaac ttaatgttgt tgataaaatt attgaagaaa taaagaataa
     6301 atttgaagaa gaaaaaattg aattgctttt tgtaagaagg gagtaaaaaa tgattaaaga
     6361 agtggataat gtagtagaaa ggattaaagc ggatataatt aactggagaa gggactttca
     6421 tatgtaccct gaacttggat ttgaagagta cagaacttct gagaaaataa aggaggtttt
     6481 aaaaaaaaca ggtataaaat atgaagtcaa agggaagaca ggagttattg gatatataaa
     6541 taaaaatttt tcaaagacaa taggaataag agcggatatg gatgccttac caattgaaga
     6601 acaaacaggt cttgaatttt cttcaaaaaa taaaggcata atgcatgcct gtggtcatga
     6661 tggtcatgtg gcaacacttc ttggtgtggg tataattctg aataaattta aggatttttt
     6721 aaagtataat gtaaaactta tttttcagcc gtgtgaggaa aagctacctg gaggtgcatg
     6781 tgaattaata aaagaaggtg ttttagaaaa tacagatttt cttattggtt ttcattttat
     6841 atccgatatt ccacttaata aaatttatat tgagaaagga atagttatgg caaatacgga
     6901 tagattcagg ataataatta aagggaaggg tggtcatgga agtgcacctg attcaacaaa
     6961 tgacccggtt gtttgctctg catttctgat aagcagttta cagacaattg taagtagaag
     7021 aataagtcca tttcagccgt gtgtagtttc tgtatgtaaa ataaaaggag gagaagcatt
     7081 taatgtaatt cctgataaag ttgaaatagg aggaactgta aggacatttt cggaaaaaac
     7141 aagaaaaaaa attaaaaatg aaatgaaaaa tatttgtgag aaaattgtct cttcatttaa
     7201 atgtaagatg gatttttctt atgaatctta ctcacctgct gtaaaaaatg accctgactt
     7261 tactgaaata attaagaaaa caactaaaga aattctatcc acaaagtttt tctataagta
     7321 tcatccatca atgggaggag aggacttcgc tttttattca aaaaaagtcc catctacata
     7381 tatttttatt ggaagtggta aaaaaagtgg tttgcatcac agtagtaaat tcaatcttga
     7441 tgagagagtt ttaccattta cagttaagta tttgagtaat ttaattctga aacttaaaat
     7501 ttaggaggaa aaaaatgaaa aaagtaattt ttgtctgggg cggatgggag ggacatcagc
     7561 caaaagagac aacagaaatt tttgctgaaa taatgaaaag agaaggttat gatgttgaag
     7621 tttacaatac acttgatatt tttcttgata aacaaaaaat gggttcagta aatttaattg
     7681 taataacatg gacaatggga aaaataacat cggaacagac aaaaggttta cttgatgctg
     7741 tcagttcagg tgttggaatt gctggctggc atggagggat gggagatgct ttcagagaaa
     7801 atacaggata tcaatttatg gttggtggtc aatgggttgc tcatccagga ggaatagttg
     7861 attatgaaat aaatatagta aaaaaagatg acccgatagt aaaaggtata agcgatttta
     7921 aacttaaatc agagcaatat tatatgcatg tagacccttc aaatgaagtt cttgcaacaa
     7981 ctactttcag gaatatagaa tttccatgga tagatggtac agttatgcct gctgtatgga
     8041 aaagaatata tgggaaagga agggtttttt atgcatcatt cggtcatcag gtaagtgatt
     8101 ttgaggttcc ggaagcaaga gaaattgtta agagaggact tatatgggct gttggagatt
     8161 tataaatttg acataaaaaa aaacaggtgt aaaataaaaa ataaaaatgg gggcgtaaga
     8221 tgaaatttat taagtttata atagcagggt taatagcagt aagttttttt ggatgtatgt
     8281 gtccaacaaa aataaaaaag gaattccttg aagaaaagga aaaagaagaa aaaccagaat
     8341 tgacgagcat tttaaagccg gaagaaaaga ttgaaccaaa accagcagaa gaaacgaaac
     8401 caacaaaacc agatgatgga aaagcaacac cagaagaagt taagaccgca attaaagagg
     8461 gagaaattcc agtgacacct ataccaccag aggcaaaatt tataaaacca gaagaaatgg
     8521 attcagaatt aaaagagatt ttccgaaata tttattttga ttttgataaa tatagtataa
     8581 gagaagatgc agcacagata ttaaaaaaaa ttggagatta cttattaaaa aatcctgata
     8641 taaaaatttt gatagaaggt cattgtgatg aaagagggac aagagaatat aatcttgttc
     8701 ttggagaaca gagagcattg agtgcaagga gatttcttgt tattatgggt gtttctccaa
     8761 aaagattata tacagtaagt tatggagagg atatgcctgc agaccctgga cataatgaag
     8821 aagcatgggc aaaaaataga agatgtgaat ttaaaataga agtgaaaaga tgagaaaaaa
     8881 aatacttttt ccaattttga gtttactatt aataatttcc ttttttaccg gctgttctga
     8941 tatagcgaca aaagatgata ttgatgctgt aaaagaaaaa ataagtaatc ttgaagatga
     9001 tttttatgcg actgttaagc agataagcga gaaatttaat aacatagaaa atgattataa
     9061 gcaaaaggat gataatataa agaaaaacat agatgaagtt tccgatagaa ttttggtttt
     9121 gagtaatgaa atgaattcta taaaagatga aattaatgta atgaaaggaa aaatagatga
     9181 aagcaatttt gaaaatagtg aaaaaataaa gaagttgaat aatgagttta ttgaaaaaaa
     9241 ccttgaaatt aaaagagaaa tagaatggat aaaaaaagat atagaaaatg taaaactttt
     9301 atataatgat ttttctttaa aaatctcaac tcttactcag aatctttcag aaacacagac
     9361 aaatattaag acagatataa ttagtttaag agattcacag gtaaaaattg ttgaaacatt
     9421 acagaacttg ccgaaaaaaa ttgaccagat tgaagaaaaa attaatgttg tggataaaaa
     9481 atttaatgaa gttgttaata gttttcttga tgaatttgca aggcatgaaa gtgaaatata
     9541 tcaattaaaa aaacaaattt cagaaaatga aaaattatca gaaagaaata cttctgcatc
     9601 aacttcttca attgtaaaat caattttaaa gacaggaaag gaaaaatatc atatagttca
     9661 gaaaggagaa tatctgactt tgattgccaa aaaatataac acttctgtaa gtgaaataaa
     9721 gaaattaaac aacttaaagt cagacgtggt atatcctgga cagaaacttt taattccata
     9781 gatttttgac actctaaaaa atttatgtta aaataaagtc caacctaaaa aataaaaaat
     9841 cttgaaaaag ggaggataaa tggaggagaa ttatatcaaa atttttaaaa agacattgag
     9901 ggaagaagaa tttgaaaaat tgaaaaaaat aaaaagtaaa aaagttttga attttatagg
     9961 tgaaagcatt ttattgtgtg aacctgatga tgtttttata tgcagtgata cacctgatga
    10021 gatagcacat ataaaaaata tggctattgt cagcaaggaa gaaagtgctg ctttactgac
    10081 accgggacat acttttcact ttgatggtta ttatgaccag ggaagggata gagaagtcac
    10141 aaaattcctt gtgccagaag gtgataaatt gagttctgca ttaaaccaga tagaaagaga
    10201 aaaagggctt aatgaagtaa agaatttatt gaaaggttca atgaaaggta aaacaatgat
    10261 tgtcagattt cttattcttg gacctgcaaa ctcagttttt acaataccct gtgttgaatg
    10321 tactgatagt tggtatgttg cccattctgt tgatttgctt tatagacatg gatataaaga
    10381 gatatgtaag atagatgaaa atacagatat attttttact cttcattcag caggagaact
    10441 tgatgaaaat atgacaagta aaaattatga taaaaaaagg atttatatag attacacaga
    10501 aaacacagtg tacagtgtga atactcagta tgctggaaat tcagttggtt tcaaaaaact
    10561 tgctttacga cttgctataa gaaaagcaca taaggaagga tggcttgctg aacattttat
    10621 gataattggt gtatatggac ctgggggaag aaagacatat attgcgggtg tttttccaag
    10681 tgcctgtggt aaaacatcaa ctgccatgct tgagggagaa aaaattttat ctgatgacct
    10741 tgcatatgtg agaaatatag atgggatatg taaagcagta aatgtggaat gtggtatttt
    10801 cgggataatt cagggtgtta atcctgttga tgaccctttg atatacagaa caataacttc
    10861 tccaaatgaa ataatttttt ccaatatttt ggttaaagat ggaaaaacat ggtggcttga
    10921 tatgggatgt gaattaccag aggaagggat aaattactca ggcaagtggt ataaaggtaa
    10981 aaaagatgaa aaaggagaag agattttacc atcccataaa aatgcaagat atacgatttc
    11041 tctcagggca ctttctaact gtgatgaaga acttgaaaat ccagaaggtg ttgaacttgg
    11101 aggtattatt tttggtggaa gggattacag aggttatgtt cctgtccagc agagttttaa
    11161 ctgggctcat ggaataattg catatggagt agcacttgaa acagaaacaa cttttgcaat
    11221 agtaggacag gaaggtaaat atgaaataaa cataatgagt attcaggatt ttgtttcaat
    11281 tcctcttgga caatatatta aaaattatct tttatttgga gaaaaacttg ataaaaaacc
    11341 tttagttttc ggtgtgaatt attttttaag gtcattgaaa acaggggatt tcttaaattc
    11401 aagaaaggat aaacatgtct gggtaaaatg gatggaatta agaatccata atgatgtaaa
    11461 ggcgattgaa acaccaacag gttttatccc tatatatgag gacttgaaaa ttttattcaa
    11521 tgatgtcttg aaaaaagaat attctaaaga agattatatt gaacagttta caataagagt
    11581 tctagaaaat ttaatgaaga ttgaaagagt tttaaaattc tgtagagaaa aaattgaaga
    11641 agcgcctgat gaagtttttg aagttttaaa tcagcagaaa gaaagattaa taaaggcaag
    11701 ggaaaaattt ggtgattata tttctcctga aagatttatt taaaaattaa aaatggagga
    11761 ggtatggaac ttttatctga aagagcaaaa ttgttaaaac catcagcaac aatggcattg
    11821 aataagaagg caaaagaaat gaaggcaaaa ggacttgata ttgtaaatct gactgttgga
    11881 gaacctgatt ttgatacacc tgaggagatt aaggaatatg caataaaagc gatgaaagaa
    11941 ggttatacaa aatatacaga agaaaaagga ttaaaagaat taagagaagc aatatgtgaa
    12001 aaacttgaaa aagagaataa acttaaatat gaacctgaag aaattgttgt ttcaaatgga
    12061 gcaaaacatt ccctttttaa tatttttcta tcaattttaa atcccggaga tgaaattata
    12121 atacctgttc cttactgggt aagttatcct gcaatggttt ctatatgcgg aggagttcct
    12181 gttttctgta atttcaatga aaaatttaaa atagacatag atgacttaaa gaaaaaaatc
    12241 acaaaaaaaa caaagggtat aattttaaac agtccttcaa atccaacagg tattgtatat
    12301 gataaaaaag aacttgaaga aattgcagaa ataattattt caaataaaat tttatgtatt
    12361 tctgatgaag tttatgaaaa aattatattt gacagtgaat atttttcaat tgcttcactt
    12421 tctgatgaaa taaaggagtt gacagttgtt gtaaatggtg tttcaaagac atttgcaatg
    12481 actggctgga gaattggata cacagcatca aaaagatacc ttgccgatgc ttttgggaaa
    12541 atacagggac agacaacttc tgcaccttca acaatatccc agaaagcatc aatttatgca
    12601 ataaaagaag gagaaaaatt atacagaaat atgattgaag aatttaaaaa aagaaaaaat
    12661 ttccttgttg aaaatctttc agaagaaatt aaatttcctt atccagaagg tgctttttat
    12721 atgtttttga actttaaaaa tatggattca aaggactttg ccgaaaaact tcttgaagaa
    12781 aaacttgttg caacagttcc tggatgtgaa tttggagttg acaattatat aagaatatct
    12841 tatgctacaa gtatggagaa tttaaaaaaa gcagttgaaa gaataaatga atttgtaaag
    12901 gagaataaat gagaaaaata tcaattttaa tttttttgtt ttgtccattt tttttgtttt
    12961 ctcaggaata taaattcaag cagtcaaaac cactcaatta tacttttcaa atgaaaggag
    13021 atgtcaactt aaattatcct ggaacaaatg aaaaatttaa tgtttttgca aaaggaaatt
    13081 taaaaattga aactcttgga attgaagaag aagtatatat tcttaaaatt actccattta
    13141 aaacaattgt aaaagttgat gaacaggtac ttgaagatat aaccaatatt gaaacaggaa
    13201 tttcttctgt tatatcaaca agtttagtaa aaatgaaaaa taatggtgaa attattgaaa
    13261 tggcagagac aaataaaggg attctttctc tttctcaggt tctaaaactt cttcctgttt
    13321 tctctgaaaa tttatcttct gggaaaaaat ggaaacagac gatacctgct tttaattttc
    13381 caggaattcc aatgtgtgca cttgaattta attattcata tgagaaaaaa ggaaattcag
    13441 gtttaattca tttaacagga atacagacaa taaaagaaac aaggaaagac aaagatacga
    13501 aaataacatt tgacggtaaa aattcttcta agggaagttt tgtttttaat gaggatgaag
    13561 gcgagataag taattttaac gggaattttt tacttgacct taacacaaaa tttgaaattc
    13621 ctccctctcc tgatgtaaaa gaaaaaaaaa tagaaaccct tacaataaag ataaaactta
    13681 atctaaatat aacactttct aaacttacct cttgatattt taaaaaacct ctacttattt
    13741 atccttttgt aagtaagaaa tgtaaaataa aaatttttga catctttaaa tctgtcctat
    13801 taaaataata acttaaccaa ggaggcaaaa atgatttata aaaatgttga attgtataat
    13861 gtagaagaaa taagggaaga taatgatgga ttacagttat tgagaattcc tgaaacactg
    13921 agaagtaaat tgaatgacaa tgctaaaaat gcttctttat atccttcagg atgtgagata
    13981 agatttaatt taaaaagcga aatatgtaaa attgtcttaa aaggaattaa tggaaatgga
    14041 atttgtgaag ttttctttgg tgattttttt gaaagttgga gattgattaa aggagaacca
    14101 actgaaattg aaataagata ccctcaaaat attgaatatc tttcaaaact ttcaaaagaa
    14161 aaaaatttaa aatttgaccc gaaacttgta agagttatac ttccacatct acttatcaca
    14221 aaaattgttg atataggagg agaatttgaa cttccaagaa aaaatcagac ccctaaggtt
    14281 aaatatcttg cctatggttc ttcaattact catggagcta catcattaag accaactgga
    14341 acatatgcat caaaaatagc acagatacta aatgtggact taataaatct tggttttggt
    14401 ggtggcgctc atcttgaaaa agaaattgct gattatattg ctgaaagaga tgattgggat
    14461 tttggaactt ttgaactcgg aataaatatg gttggtggtt ttgaggttga tgaatttgaa
    14521 aagagggttg attattttat tgaaaagatt gttgataaaa aacctgacaa atatttattc
    14581 ttcatagata tatttccttt ttatatggac tttgacaacc aagaaaaaca ggaaaaattc
    14641 agggagattg taaaaaataa agttaaggag gtaaataaag ataatgtaat tcatatttca
    14701 ggatttgata ttttaaaaag tttaaaaggg ctaacctttg atattcttca tccttcacct
    14761 tttggtatgg aagaaatggc ttataatctt gcgaatatta tgaaaagtta tgggttggta
    14821 gattaaaagg gagggaaaaa tgctttacag aaatattgag ttttataatg ttgaggaaat
    14881 ttttgaaaga gaaaacggac ttcagttatc aagaattcct gaaagagtga gggttaaact
    14941 gaatgataat gcaaaaacaa gagcgattat gcctgcaggg tgtgaattaa ggtttagatt
    15001 gaaaagtcag aaagcaaaaa ttgtgatgaa aacagataca gaagttgtct ggagaaatgg
    15061 aatatgtgag atttattttg gggatttttt tgaaagatgg tattttgtaa gtgaaaatcc
    15121 aagtataatt gaaattcaat atccacagaa tcttgaaaaa ttaaaagaat taaagaaaga
    15181 gaaaggattg aagtttgacc ctgaagttgt aagaattata ctacctcatt tagttgattt
    15241 aagaattctg gagattgaag gagaatttaa tccacctgaa aaaaatcaag tccctgataa
    15301 aaaatttatt gcctatggtt cttcaattac tcatggtggt ttttcaattg ttccaagtta
    15361 tacttatgtt tcgaagacag catatattct cggtatggat gcgataaatc ttggtttagg
    15421 gggaggagca catcttgaaa aagaaattgc tgattatatt gctgaaaggg atgactggga
    15481 atttggggta tttgaacttg gagtaaatat ggttggtggt tttgaagttg accagtttga
    15541 aaaaagggtt gattattttg ttgaaaagat agtaaaagag aatcctgaaa aatatctgtt
    15601 ttttattgat atatttccat tttatatgga ctttgataat caggaaaaac agaaaaaatt
    15661 cagggaaatc gtaaaaaaca aagtaaaaca gataaaaaag aaaaatgtaa ttcatatctc
    15721 aggttttaaa attttgaaag atgtaagagg ccttatgaca gatagagtcc atccttcatc
    15781 acagggtttt gaggagatgg gatacaatct tgcttcaatc ctgaaaaaat atgttgtttg
    15841 aaagagaaaa atagttcagt gtaaaaaagt aaattttatg agaaaatatt tgaaagaact
    15901 tttaattatt tcatttcccc tcattataag ttcaagtata tggactattc agcattttgt
    15961 tgatagaatg tttatttcat ggtattcagt taaagcactt gcagcagtat tacccgcaag
    16021 ttttttaaat ttaacaacta caagtatttt tgttggaact gcctcttttg tgaatacttt
    16081 tgttgctcag tactacggag ccgaaacata tgaaaaaaca ggtgaaattg tatggcaggg
    16141 tttatatctg gctcttatag gtggcatttt acattttctt tttattccac ttactccatt
    16201 tatgttttca atttttgctc atgaaaaaga ggtcttaagt tatgaagtta tttattttca
    16261 gattctctgc gttggtgctt tttctgtaat tgcttcttca tctttatctt catttttttc
    16321 aggaagaggg aaaacaaata ttgttctttt tgtcagtttt ctccagacaa ctttaaatct
    16381 gatttttgat taccttttga ttttcgggaa atggatattt ccggagatgg gaataaaagg
    16441 tgcaggtatt gctacagtta tttctggata tttttcattt tttgtttatc ttttattaat
    16501 ctttcagaaa aaaaatttaa tgtatgggat taaaaatttc aggtttaata aagtactttt
    16561 taaaaatctt ttaaaatacg gtgttccaag tggtatgcat ttttttcttg atatttctgt
    16621 ctggactatt tttttactta taataggtaa acttggagtt attcctcttt cttcttcaaa
    16681 tatcgctttt aatataaata cagttgcttt tatgccgatg ataggaatag gtacaggtgt
    16741 ttcaatactt gttggtcagt atattgggaa agggaaacca gagataggag aaaaaataac
    16801 ttatattgga tttttgatta cttttatata tatgtgttca attgctctta cctatattat
    16861 atttcctgat ttctatatta agttttttag accgcgggtt tatacaggtg attttgagca
    16921 ggtaaggaat ttaacagtta tacttttgag gtttgttgct ttttactcaa tttttgatac
    16981 tatgaatgta atttttcttt ctggactgag gggtgcagga gatacaaaat ttattatgaa
    17041 tgttttttta tttgccacaa tttttatttt tcttattcct ctttatatct gtactttcat
    17101 ttttaattca gggatattta taggttggtc tattgcaacg ttatatgtga tagttatagg
    17161 gttcagtttt tttataaggt ttttaaaagg aaagtggaag aaacttaagg tgattgagac
    17221 acctgttctg ttatgatata attttaatcc caccttatga ggtggggaat tagtaggaag
    17281 gataatgaag aaaaagaaaa tgataaaaag gagggagaat ggaagataga gaaaagatgg
    17341 ttagttttct tgcttatttt tttggttgga tttcaggact tattattttt ttaacggaaa
    17401 agaaaagtga atatataaga tttaatgcta tgcagagtat aatttttagt ggagttttaa
    17461 ctgttattta tattttcatc tctattgttt ctctcattcc tttcataggt aaaattctat
    17521 ctttaattct ccatcctctt gtttatcttg caggaacagc gatatggatt gtgcttctta
    17581 ttaagtcatt caaaggagag tattttaaac ttcctgtaat tggcgattat gcagagaaat
    17641 attcaaaaac gctatgaccc ttttgtccac ctttaaggtg ggatgtggaa atgaaggcag
    17701 gacaaggaat gagaaaggca gttagatagg agtgaataaa tggaagaggg aagtttgttt
    17761 catgtattta caaaaagtat tgctggctat aaagttttta aaagtaaggt tgattacagg
    17821 aggatggttg agacaatctg gtattatatg gatgaaagaa cggcaaaatt ttccagagtt
    17881 attgacttaa aaaaagaaaa taaaggagag ccagttgtaa gaataatatg ttactgcata
    17941 atgcctacac atttacacct tgttttagaa gaaaaaaaag aaaaaggaat ttcacaattt
    18001 atgagtaata ttttaaacag ttatgcgaga tactttaatt taaaatataa aagaaaggga
    18061 catttatggg aaagtagtta taaaagggta ctggtagaaa cagatgaaca gttactccat
    18121 ctgacaagat atatacattt aaatccgaca accgcaggca tagtgaagaa accagaaaac
    18181 tgggagtttt cttcatacag ggaatattta ggtaatgaaa agattttttt aaaaatatgc
    18241 tattttgatg atattataga aataaatcca aaaagttata gggaatttgt agaggattat
    18301 attgattacg agaaggaatt acacaggata aaagggttgg taaaatttga atctgaaaat
    18361 ccttgacaga caaaggtttt ccaagcccac cttaaaggtg ggagggagga ggaaaaatga
    18421 aggtggcgta tcttgttgga aaaaagaaaa tagttataga ggaagaaaaa aatccagaga
    18481 ttgaaagaga agatgatgtg cttgtaaggg ttagatgtgt tgggatttgt ggttctgatg
    18541 ttcattattt tcttgaagga agaattggag accagattgt gaaggataag ataattgttg
    18601 gacatgaggc aagtggagag gttattgagg ttggaaaaaa tgtaaaaaag ttgaagaagg
    18661 gagataaggt tgcaattgag cctggaataa gttgtgggaa atgtgagttc tgtataaagg
    18721 gaaagccaaa tatatgtccg aatgtaaaat ttctcggtac tcctccggta aatggtgctt
    18781 tcagagaata tatggttatg cctgaggaaa atttaatcaa aatacctgat gggcttgaat
    18841 atgaagaagg agttttatct gagccacttg cgataggagt ttatactgta aaattaagtc
    18901 agattgaaac cggagatgat gttgctattc ttggagcagg accgattgga ctttctattc
    18961 ttttttcagc aagaataagt ggagcaaata gaatttttgt ttctgatttg ataaaggagc
    19021 gacttgaatt tgcagaaaag ataggggcta attatactgt tgatgctaaa aagaatgata
    19081 ttacagaaat tgtgaaaaaa atcacaaata atagaggagt tgatatttct tttgaagcag
    19141 cagggaaaag agagacattt aggcaggtta ttcatacaag caggattgga ggaaaatgtg
    19201 tttttgttgg aattcctact gaagatacag ttgagtttga agcacatata atgagaagaa
    19261 aggaacttaa acttataaat ataagaagga gtgctttctg tacagagatt gctttaaatt
    19321 tgatgaagaa ttcagaactt ccttttaaag agatgataac gcatagattc ccttttgaaa
    19381 aaattgaaga agcacttaat cttgttgcag aatacaggga tggagttata aagtgtgttg
    19441 tcaatatatg agggaggggt aaaattaggt ctcaaaaagg ttgaaagaca aggattccca
    19501 atgtccacct taaaggtggg cagaggagaa ggtgggaaga ggaggaggtt gggaggaagg
    19561 aggcaaggat ggaagtagta aagaaaattg aacaagtaag gaagattgta ggaaaggcga
    19621 aaaaagaggg taaggttatt ggatttgtac cgacgatggg atatttgcat aaagggcata
    19681 tttcattgat aagaagagca aaaaaagaat gtgattttgt tgctgtaagt atttttgtta
    19741 atccgacaca gtttggaccg aatgaagatt ttgatagata cccgagagat tttgagagag
    19801 ataggaaaat tcttgaagag gagaaggtgg atttagtatt ttttccagaa gttgaagaga
    19861 tgtaccctta tgattttaaa acttgggttt atgttgaaaa atattctgaa atacttgaag
    19921 ggaaatttag accgggtcat tttaagggag tttgtacggt tgttattaaa ttgctcaata
    19981 tagttcagcc ggataagagt tattttggat ggaaggatgc acagcaactg attattgtaa
    20041 agaagatggt tgaggatttg aatttaccat gtgaaataat aggatgtgaa acagtaagag
    20101 aagatgatgg acttgcagaa agttcaagaa atgtttattt gaatgaggaa gaaagggaaa
    20161 gagcgacttg tttatacagg gcacttaaaa gaattgaaga gatggtgaaa aaagaaaaga
    20221 tatatgattg taaaaagtta attgaagagg ggagaaaaat tatagagaag gaaaatgtag
    20281 aaattcagta tcttgaaata gttagaatat ctgaccttat gccagttgaa aaaattgaaa
    20341 aggatgttat tgttcttggt gcggtaaaaa taggaaatgt aaggttgatt gacaatataa
    20401 gattaaatgg atttgaaaac ctttgataat caggcgactt ccagtcccac cttaaaggtg
    20461 ggaaaaagtt gacttgtaaa ggtgggaaaa agttgacttg tgacttggtt ttataaatag
    20521 gtaagatata aaaatgaaaa gaaaaattaa aacaaggggg agagatatga aaacttttaa
    20581 tctccccaag gccacctcgt aggtgtctaa acgggaaaaa tgtgatataa tatttttaag
    20641 atgaaaagtg aaaattttta tcatgttttt acaaagagta tagcagggta taaaatattt
    20701 gtgaaagagt cggactatag gagaatgcag gagataattt ggtattatag atatgagagg
    20761 aaaaataaat tttcaaaggt gaaggatttt atgagtgagg attttggaga taaacttgtt
    20821 gttatagtga gttattgtat aatgccaaca catttacatt ttacacttgg agaggaaaag
    20881 gaaggcggga tttcaaaatt tatggcaaat atattaaata gttatacaag atattttaat
    20941 ttaaaacata ataggaaagg acctttatgg gaaagcaggt ttaaatatgt tttaatagag
    21001 acacaggaac agttattaca tttaacaaga tatcatcatt taaatcctgt tgttgcagaa
    21061 ttagtgaaaa agccagagaa ttggaaattt tcttcatata gggaatattt gaattatgac
    21121 ggaggactta aaatttgtga gtttgaagaa ttgatagata tgagttcatc tgaatataaa
    21181 agatttgtgg aggacaatat tgattatcag aaagagattc ataaaattaa agaacttatt
    21241 aaacttgaaa cactttgagg gacaaggatt tcccgaggac acctacgagg tgggaggagg
    21301 ttgaaaatgg aacttacaaa agaattatat gaagcgataa taacgatagt tgaggataag
    21361 gtaaaggata taaaggttac aagagaggag tttgagttat tgagaaaggc agttttagat
    21421 ttgacagagg cacagaggaa agcagaggag aggttgacaa gattagagga agcggttgag
    21481 agattagcag aggcacagaa gaaagcagag gagaggttgg taagattaga ggaagcggtt
    21541 gagagattag cagaggcaca gaggaaagca gaggagaggt tggtaagatt agaggaagca
    21601 cagataagga cagaagagag gttggtaagg ttagaagaag cggtagagag attggcggag
    21661 gcacagagga agacggaaga gagagtaagt agattagaac aagtggtaga gagattagag
    21721 caggcagtag aaaaattagc agaggcacaa aggaagacag aggagagagt gggtagattg
    21781 gaagaagcgg tagaaaaatt agcagaggca caaaggaaga cagaggagag agtgggtaga
    21841 ttagaagaag cgatggagaa attagcagag gcacagagaa ggacagaaga gaagttggga
    21901 aagttagaag agacacagag gaatttacag atagagatag caaaactttc agatacaatt
    21961 ggatttggaa ttgaagatgt tgcaaaagtt gttttacctg gatatcttga gagacattat
    22021 aaaataaaaa taggagagat agagagaaaa tttttccgaa tagaaaataa ggaaatagag
    22081 gttaatcttt atgctgaagg aaaaaaggga agtaagaaaa tttatcttgt cggagaggca
    22141 aaatcaagga tatatgaaag ggatgttgaa aaatttataa gtgagaatat gttatttatt
    22201 gagaaatttg gagagaagaa tataataaaa gttctttttg ggttttacat tcatccatct
    22261 gcttctgaaa ttgcaaagaa aaataagata ttacttgttg cttcttatat gagataatta
    22321 gatatgaaaa atattgagag acaatagttg ccaaaggaca cctacgaggt gggggaggga
    22381 aattgacaaa agtttaattg gtgatataat taatacaaaa aaggagctaa attgaaaatt
    22441 tttaaatttt ttcttttctc tttcttcctc ttattttgtt taaatgtttt tggtcagcct
    22501 cacttttcat attctttgag tggtgctcat attagaggga tatatcgcgt tacttgggag
    22561 gagtctaatc ctcctagtcc tccttataca ttagagattt ctatagacgg aggttccact
    22621 tgggattttt tatgtagtgg atcgaacacc tattttaatt ttaatacaga aggaaagccc
    22681 aatggtcctg ctatgttgcg aataatagac catgaaggtg gatctgatca agtaaacatc
    22741 accatagata atactccgcc agattcagat ataagaattt ttgggactgt aagggttgga
    22801 agtacaatta gttatacagg aagttggagc gataatttgt caggacctgt tatagttaag
    22861 gaagcatggt tatatggaga tacagatact gatacaagta atgctgtaaa gattaaagat
    22921 ttgactattt cagaaacg
//
LOCUS       DAOSAT010000016        22877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_1622_length_22877_cov_4.808080, whole genome shotgun sequence.
ACCESSION   DAOSAT010000016 DAOSAT010000000
VERSION     DAOSAT010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22877
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1622_length_22877_cov_4.808080"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1110
                     /locus_tag="PLF90_02400"
     CDS             61..1110
                     /locus_tag="PLF90_02400"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOK56169.1"
                     /translation="MRTPFQRDRDRIIHSTAFRRLEYKTQVFIYHEGDYYRNRLTHTL
                     EVQQIARTIARELKVNEDLVEAISLAHDLGHTPFGHKGESVLNEIMQLHGFPGFEHNR
                     QGLRIVDYLEERYPDFPGLNLTYEVREGIIKHMTSYDIPLIGKEFEEFIKFKSPTIEA
                     QIVNIADEIAYTCHDLDDGIKSEIITYKDVEDVELWKEIERISDLDKQPEKHKRHIMI
                     RNLINYLVVDLIEETKKNLSKINPQSVEDVRNSEIIVKNSEKVRTQHAELKKFLEEKM
                     YKHPKVIRMTEKGGRIIRELFYAYYNEPRQLPYHIQKKIEKEPKEIVICDYIAGMTDR
                     FALSEYQKLFDPTSF"
     gene            1113..2180
                     /gene="dprA"
                     /locus_tag="PLF90_02405"
     CDS             1113..2180
                     /gene="dprA"
                     /locus_tag="PLF90_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOK56170.1"
                     /translation="MDEKIYWIALNLIGISYVKLKKLLEELKRVEEIFKLNTKKLIEL
                     GLSKNIAEKIERWEELPLEEEIKYIENEGINIITIDDKNYPSLLKEIYDPPFLIYFKG
                     KLDLNAPSISIVGTRNPSVYGLKMAEKFAFELASYGFIIVSGLARGIDTSAHIGAIRA
                     NGKTVGVMGSGFKNFYPPENKKLEKDMIKNGAIITEFPSYTLPEKYNFPKRNRIISGL
                     SKGLIVIEAGPKSGALITANFALEQGKDVFALPGRVDTLYSKGTNKLLKEGAILIEDV
                     KDILETMNIEIENIENKKEKIILESEEEKNILSVLEQTSSIEEIQMKTKIEPNILLSL
                     LTNLQIKGLIEELPGKLYRKK"
     gene            2260..3399
                     /gene="proS"
                     /locus_tag="PLF90_02410"
     CDS             2260..3399
                     /gene="proS"
                     /locus_tag="PLF90_02410"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020033459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOK56171.1"
                     /translation="MKWSKYFIPTQKEAPKEAETISHKLLIRAGLIDKLASGVYTYLP
                     LGLKVLRKVENIVREEMNNSGAIELLMPSLQPATLWKETGRLDKMGKDMIKFIDRHER
                     EMIFGPTHEEVITDIVRRYIKSWKQLPIILYQIQTKFRDEIRPRFSIIRSREFLMKDA
                     YSFDINEEGLDKSYILMKEAYKKIFERCGLKFTIREAESGVIGGKFSEEFVADGECSE
                     LEVGHIFKLGLEYSKSMKAYFVDRDGKEKPIYMGCYGIGVSRIVAAIIEGNYDKDGII
                     WPISVAPFLADIILTNKEDDKIVNTSEKIYNGLKENGFDVIFDDRDESAGVKFKDADL
                     CGFPYQIIIGKKIKDDKVEIKERKTGKITEINPDKIVEYFKTLKN"
     gene            complement(3425..5143)
                     /gene="thrS"
                     /locus_tag="PLF90_02415"
     CDS             complement(3425..5143)
                     /gene="thrS"
                     /locus_tag="PLF90_02415"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOK56172.1"
                     /translation="MDYKKYWHTTSHIMAQAVKRLFPEVKLGTGPAIDKGFYYDFYRD
                     KPFTPEDLEKIEEEMRKIIKENFEIERFEKNKKEAEEILKNEPFKLEILKDINNEIVS
                     FYKQGEFIDLCEGPHLNFTGEVKFFKLLSVSSTYWRGDENRESMQRIYGISFETKEEL
                     DEFLKFIEEAKERDHRILGKKLDLFSIHPEYGPGLIFWHPKGAIIRKIIEDFWKEVHL
                     KNGYELVYTPHIADISLWEKSGHLSFYSEYMFPRMEIENGRMYQLKPMNCPFHIQIYM
                     SKIRSYRDLPIRWAELGTVYRYEKPGVLHGLLRVRGFTQDDAHIFCTPEQLEDEVCKI
                     IELVYFFLSSFGFKDYRVFLSTRPEKFVGTVENWERATKALENALKTKNINYEIDPGE
                     GVFYGPKIDIKIKDCLGREWQCSTIQIDFNIPEKFEIKYIDKDGNFETPIMIHRAILG
                     SLERFFGILIEHYKGNFPLWLAPEQIRILPIADRHNEFCLNLKKKLEEDFRTGVDLRN
                     EKINKKIKEAEDEKIPFMIIIGDKEIKENKLSVRKHGSGMLGYFTLEEVVEIIKKDIQ
                     LRKRLF"
     gene            complement(5166..5239)
                     /locus_tag="PLF90_02420"
     tRNA            complement(5166..5239)
                     /locus_tag="PLF90_02420"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5203..5205),aa:Asp,seq:gtc)
     gene            5359..5724
                     /locus_tag="PLF90_02425"
     CDS             5359..5724
                     /locus_tag="PLF90_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOK56173.1"
                     /translation="MEKYTEIFKTVGEKTRLKIVKLLLKSEVPLCVCEIMDCLKENQT
                     NISKHLKILKYAGIVEEKREGKFVMYSLTKSEDRFLLSLLNTINSIPENIFKEEIECL
                     KKRLNLRENGKCVIGIRRK"
     gene            5726..6772
                     /locus_tag="PLF90_02430"
     CDS             5726..6772
                     /locus_tag="PLF90_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOK56174.1"
                     /translation="MIKEIIVGGFLALKDYIATHVLTCLIPAFLLAGGMVSFINKEII
                     INYLGEKVNKIKSFSIAGISSFLIAACSCTVIPVASGLYYTGAGIGSAFIVLWVAPSS
                     NILSLIYTGTIIGGQIVISRVISALIMAFVVGSVMSFVFRKEKIEEIKLEKEPEKKII
                     ERNYLILLILILLSLLAPNYIVQKGPYWKKVIVWFIFTLFVAIFAFKKIEKEKIKDWL
                     RETLFFVRMIFPLLLIGVFIVGIIGKILPSNWIERFLGGNSLRSSFFATLIGATTYFA
                     TLTEAPFVDTLMKLGMGKGPALALLLTGPGLSLPNWIAISKVFGVKKAIVYVITIVFC
                     GTFVGWFFGNFILK"
     gene            6786..7019
                     /locus_tag="PLF90_02435"
     CDS             6786..7019
                     /locus_tag="PLF90_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOK56175.1"
                     /translation="MEILIVGAGCPKCQAVEKVVREVVEELKIEANVSHLYDIKEFPK
                     YKVTITPAIVIDGKVVFAGKIPSKEEVKKLLRK"
     gene            7036..7389
                     /locus_tag="PLF90_02440"
     CDS             7036..7389
                     /locus_tag="PLF90_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56176.1"
                     /translation="MKPRFIMCICTGQCPGFKSLDLWSLINFVRNELDVEYAIVHPQL
                     CVDDGDRFIKDYIKPDVKYVIGACDPKMQRKMFKDAFMEAGGDYDKQVISLDLRNMPT
                     EEAMKKVKEAVESIK"
     gene            7414..7731
                     /locus_tag="PLF90_02445"
     CDS             7414..7731
                     /locus_tag="PLF90_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOK56177.1"
                     /translation="MEKDRSTYTGLPRKKIDWFPKIDYSKCKPEECGYHCVNFCPFKV
                     FSKNENDQKVIVSQPYECNVGDESCKFQCPFGAISFPTREELKMMLKKVREKYEKQGG
                     KNV"
     gene            7724..7981
                     /locus_tag="PLF90_02450"
     CDS             7724..7981
                     /locus_tag="PLF90_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK56178.1"
                     /translation="MFKNIPREKIPWFPTVDYEKCIGCKECYNFCKNGVYEWDEEKNK
                     PIVKNPYNCVVGCSACANLCNGEAISFPKMSDIKKMLEKEK"
     gene            7989..8693
                     /locus_tag="PLF90_02455"
     CDS             7989..8693
                     /locus_tag="PLF90_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOK56179.1"
                     /translation="MENISYIGSFILGIITCLSPCSIAILIAVLSFIISEEESIKNGI
                     YIGIFFTLGMSLVFFILGIFISKIGQLLKFSHYFYLISGILLIFFGLLNLGIFKKKKT
                     SGSLNFFQRFGFSILSLNKYSKLLTSFLFGILFSLGWAPCATSLIMPVAILVMAQEIS
                     ILKGGFLLFLFGLGHGFPVIPLSALSGTVRTNITKKTARKGDLIIKIFGIIIILFGIL
                     FIVYGPKINTLIGGKK"
     gene            8690..9211
                     /locus_tag="PLF90_02460"
     CDS             8690..9211
                     /locus_tag="PLF90_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOK56180.1"
                     /translation="MKKLIILFTLLFLFSFAKTNKAPDFNLKDPYGETFSLSQFKGKI
                     VVLNFFKIYCGGRTAPGTLNQIEELKKICNEMCKGKKCKDGNLTIIGITLSSCSTTDL
                     KIWVEEKGIKWYVGNDLDDYNLDIIKNYAEYLKTLKDPCLIFINKNGEVVFKSNYMKA
                     DEIKKKLKELNLI"
     gene            9208..9396
                     /locus_tag="PLF90_02465"
     CDS             9208..9396
                     /locus_tag="PLF90_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56181.1"
                     /translation="MRKEVIEFEEKDIVYITRIVLDNNKEEALKFLKKIYIVLKKREK
                     SHCKTSYAWEKDIEKNQK"
     gene            9393..9926
                     /locus_tag="PLF90_02470"
     CDS             9393..9926
                     /locus_tag="PLF90_02470"
                     /inference="COORDINATES: protein motif:HMM:NF015987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOK56182.1"
                     /translation="MNITIEKILPCIADPEKIRFIASIDEDISEILPYLNVVLPKAIY
                     NHEGKCLTITNEEGIITIYPHKISAGKIKDENGAREIIKWLKEEIEYCIKNKDKIKPD
                     YTRKDKLEVLHVYKLLPQKNCKKCGENSCLAFSFKVINEEKNIINCKELFLPEYNEKR
                     KLLFDMLKESGYKVPDF"
     gene            complement(9930..>10144)
                     /locus_tag="PLF90_02475"
     CDS             complement(9930..>10144)
                     /locus_tag="PLF90_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOK56183.1"
                     /translation="EPIFAIIGALLTSIYFKILPFALSFAAGAMIYVIIEEVIPETQK
                     AENTDISTIFFIIGFLIMMILDTFIK"
     assembly_gap    10145..10244
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10245..10897)
                     /locus_tag="PLF90_02480"
     CDS             complement(<10245..10897)
                     /locus_tag="PLF90_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOK56184.1"
                     /translation="MLNKFYNFTPLLQSFYGGLFTWFITLLGASIVLFNRKPNRKFFD
                     CMLGFAGGIMLSASFFSLILPSIEYSKNLNIPQWFPVLIGFLLGSFFLFGLDKLIPHL
                     HLNTNISEKEGIRKQLPVYFLFVLAIVIHNIPEGISVGVSFGSLKYLKSKETFSSSIS
                     LAFGIGIQNFPEGLAVSLPLLASGLSKRKSFFYGQLSGIVEPIFAIIGALLTSIYFK"
     gene            complement(10897..11382)
                     /locus_tag="PLF90_02485"
     CDS             complement(10897..11382)
                     /locus_tag="PLF90_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOK56185.1"
                     /translation="MENGIKFAKEFEKHGANLYIEIGMKSENLITKKLFYSLAKQEIE
                     HLEIIENFVLSDDYKTEKTEMIEEDLKEFFTSLKEKNKIESNIDGYKMALEIEKKGYL
                     QYEKFYNEVKNEKEKRLFKFLMEQEKSHIEAIINVYSYLTETGDWFEKEESKIWNWMN
                     M"
     gene            complement(11394..11717)
                     /locus_tag="PLF90_02490"
     CDS             complement(11394..11717)
                     /locus_tag="PLF90_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56186.1"
                     /translation="MIEGKIVKIRFKKYYLEQKLWVFIGRVLKFTENWIMIEGKGIVV
                     IKGQVSPVDIDKEKRIILIPRENISHIRILPDDFDLEKMEIIEKGLRIFVKIPNGPDT
                     SISEI"
     gene            complement(11714..12121)
                     /locus_tag="PLF90_02495"
     CDS             complement(11714..12121)
                     /locus_tag="PLF90_02495"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOK56187.1"
                     /translation="MKKILFLCRENSARSQIAEGIVNYFYKDNFIAFSAGSIVKFVHP
                     LAIEVMKEIGIDISNHRSKSIKEFLNEEFDYVITVCVGDKKGLCPFFPGKTKKTIAWE
                     IPDPAEKNEIEGFRKVRDILKRKIDDFISKEEK"
     gene            complement(12118..12777)
                     /gene="phoU"
                     /locus_tag="PLF90_02500"
     CDS             complement(12118..12777)
                     /gene="phoU"
                     /locus_tag="PLF90_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOK56188.1"
                     /translation="MLEDKLKILKQKIIEFSLLIEKMIDKSIKGIINKNGIDLKEVIE
                     KLEPIANKFEIEIDELCAEIIAQYEPKAKNLRIVLMILKINNDLERIGDHAVNISESG
                     LFLIEKPDVKPLIDIPRMADETIKMVKDSINSFIEENEKLARDVCKRDDIVDNLKDQI
                     TRELITYMIADYTTIERSLHLIRIAQNLERIADLSTNISEDVIFMTEGKIIKHHIEEN
                     K"
     gene            complement(12792..13550)
                     /locus_tag="PLF90_02505"
     CDS             complement(12792..13550)
                     /locus_tag="PLF90_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOK56189.1"
                     /translation="MESEIIKIEDFSAGYGNSIAIKDINLGILKNKITAIMGPSGCGK
                     TTLIRCINRMHELNKDAFHKGNIYLDGKNIFEMNTVLLRRRIGMVFQKPNPFPHLTIY
                     ENVLAGYIINGIKLRKEERDRIVEETLKKVALWEEVKDKLYLNGTQFSGGQQQRLCIA
                     RVLALNPEIILLDEPTSSLDPKSTIEIENLLEKLKNNVTIIIVTHNISQASRIADYVA
                     FLYLGELIEFGKSEKIFTAPENPITEEYLSGKFG"
     gene            complement(13532..14359)
                     /gene="pstA"
                     /locus_tag="PLF90_02510"
     CDS             complement(13532..14359)
                     /gene="pstA"
                     /locus_tag="PLF90_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOK56190.1"
                     /translation="MKIFKDKIFRFFVIFFSFLSIVPLLFFFFTILKKGFTAINWEFL
                     TSLPKPPGEIGGGILNGIVGSFILIFLASFFSIPTGIFLGLFLYEFSDKKFGEFCNTL
                     IDVLQGIPSIVIGIIAYLWIVKQMDHFSAISGAIALSLIFLPIIIKSTKESLQMIPFS
                     LIESSLSLGATYPRTILKVILPCSLNGIISGILTGIGRIAGETAPLLFTAFGNPFLNF
                     NILKPVSSLPHLIFVYTMSPYENWHKHAWGCALILVIIVLFLNSISKIGFRRWKVKL"
     gene            complement(14356..15228)
                     /gene="pstC"
                     /locus_tag="PLF90_02515"
     CDS             complement(14356..15228)
                     /gene="pstC"
                     /locus_tag="PLF90_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOK56191.1"
                     /translation="MTSEKAYKTLLGIIGGVLIIGLLLSIFFTLFINSLPSIKSFGFK
                     FFLEKTWDPVFGNFGALPFIIGTLLTSIISLIISIPFSLSVSLFVGEFYRDTFLSKIL
                     ETLIGILAVIPSVIIGLWALFYLTPIIRNIQAKLSLPPTGVCIFTASLTLSIMILPYA
                     TSVGKEVIKLVPNDLKEAGFSFGATSYEVVKKIVFPYARSGILAGFLLSFGRAIGETM
                     AVTMVIGNSNYIPKNIFSPGNTISSVIANEFLEAVENIHISSLIELALILFLITFIMN
                     FVANIIIKRMRVKI"
     gene            complement(15225..16265)
                     /gene="pstS"
                     /locus_tag="PLF90_02520"
     CDS             complement(15225..16265)
                     /gene="pstS"
                     /locus_tag="PLF90_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOK56192.1"
                     /translation="MIKKIFLGLVMLFVGIAKVESQTELVGAGATFPYPLYSKMFDEY
                     YKIYGVKINYQSIGSGGGIRQLKAKTVDFAGSDAILSDEQLKDFDDEILHIPTCLGAV
                     VITYNLPVEKELKFTPDIISDIYLGKITKWNDDKIKKVNSNVNLPDMPIIVVRRSDGS
                     GTTFVFTDYLSKVSEEWKTKVGRGTSVNWPVGIGGKGNEGVSGIVKGTPGAIGYVELT
                     YALQNNLPFGIIKNKSGNFVKPSIESITFASDIKLPDDMRVSITDTENKNGYPISAFT
                     YILVYKNLKNANEKMSYEKAENLVKLLWWMTHEGQKYTKPLNYAPLGKEAVKKAENLL
                     KKLHYDSKILIK"
     gene            complement(16279..17463)
                     /locus_tag="PLF90_02525"
     CDS             complement(16279..17463)
                     /locus_tag="PLF90_02525"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOK56193.1"
                     /translation="MRKFYIIFLFLFLIFNFSYAGEVDILMKKLIEKGIISKDEAEQI
                     KNEIKKEKEKEKEKYDFLKNTKFSGDIRVRYQNEDIEGSPDRDRYRIRARWGFKTKIS
                     ENTEIGLRLATGTGEQTSTNQTFGEAFSQKSFWLDRAYLKHNFNDFGLIAGKIENPFY
                     TTDIIWDSDINPEGLAFIYKNQKGLFINSGIFPLAEFKNLTDDPFLYGIQLGYGSKIG
                     NEVEFKIATSIYTTKSLKGKKQSEISPKYEPKGNTLESNGKYIYEYKPFDIILELTPF
                     KIAEKPFTLYADYLKNIESGVREDKGYLIGFSIGKLKEKNDWKFEYNYRKIDADATLA
                     IFSDSDINGGGTDLKGHKIGFVYQVNKNSSFGITYFIGKSLGVNRTDNRNTLQIDYLV
                     KF"
     gene            complement(17580..18110)
                     /locus_tag="PLF90_02530"
     CDS             complement(17580..18110)
                     /locus_tag="PLF90_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK56194.1"
                     /translation="MEIKIENYFPLVKKIVKRYEGIGVESEDLFQEGCIGLIEAKNNF
                     KEEMNVNFLTYATYWIKKYILESVEKYKKQKIYISKDALSFTTDTNITKFSEISYKLN
                     LEILSPFERNIIEKFYLEGKTLEEIAKELKIKREKVRQIKQKALMKLKNFNRKLTNTL
                     NGFNKKKVNNCKEEEN"
     gene            complement(18177..19859)
                     /locus_tag="PLF90_02535"
     CDS             complement(18177..19859)
                     /locus_tag="PLF90_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK56195.1"
                     /translation="MKKYLIKKFVRTSILVSGSVFILFYIIFIQTLHKIVFLNVEENL
                     KEFAFKIYNDLEKNSENKKFIEDLKKIDKYTGIRITLIDKDGTVIIDTRENPKFMENH
                     KDRPEIISAIEKGEGKSIRLSPTLNEYMFYYAVFVKEKEVVLRVSKNLTDIQKIFYEF
                     KKQFGLIFLITYLIFISIFTFMIPFFLKEFSLIDEFIEKIGKGKYGEKLLGFKQKELK
                     NIAEKLNLISEKLLLTSELEKKNVEMFEVIKFLEEPVAFFNEEGKLLNFNVNFKKFLK
                     SPFQNRYFWEVIENFEINEIIEKTMNYKKGQEKEAEIDNRWYLLKTIYLGEKIILFMF
                     DITTTKEIEKIRKYFIFNISHELKTPLTVIKGYAETIEDETGDSNIKNYAIIIKNQID
                     RLIKILENILNLSSLESKKIELEEFDLLKVVKKVYDLYKEKVEKKGLSFVLNFSDVPL
                     IKGDEFKIEQVIINLIDNAVKFTDKGKIEINLYKEKNYVIFEVSDTGYGISKEDLPKI
                     FDEFFIGSKSKPKAGTGLGLSIVKKIVDLHNGKIEVKSELNKGTKFKVYLPV"
     gene            complement(19856..20530)
                     /locus_tag="PLF90_02540"
     CDS             complement(19856..20530)
                     /locus_tag="PLF90_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOK56196.1"
                     /translation="MKKTIAIIEDEKDILQLLKHNLEKIGFNIKGFENGNDFLNYLKL
                     DRPDLIILDLMLPDMDGLEICKYLKRERKYLDIPLIILTAKGEETDKVLGLELGADDY
                     ISKPFSIKELIARVKAVLRRYQTKSDIIDIGGIVRVDLNKYEVYVKGEKINLTTTEFN
                     ILKILLSKKGWVFSREKILEELWQGEKAVIDRTVDVHIKKLREKLKEGGRFIVNVRGI
                     GYKIEE"
     gene            complement(20527..20649)
                     /locus_tag="PLF90_02545"
     CDS             complement(20527..20649)
                     /locus_tag="PLF90_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56197.1"
                     /translation="MYRLLIEKLFFTNVFAKQIIVACGEGAKSNISAFEYLKYL"
     gene            complement(20707..21276)
                     /locus_tag="PLF90_02550"
     CDS             complement(20707..21276)
                     /locus_tag="PLF90_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOK56198.1"
                     /translation="MKVIGICGSPRKNGNSEILLDEFLSYFKEKAEVRKIYLNLLKFV
                     PCQECENIRKDGRCKINDDMQNVYDEIENSDIVVVTSPIFFGSISAQTKMMIDRFQCQ
                     WLGINFFKSFKPKRKKGIFICVEASNRDDFIDNARSIIKNFFATIGASYYGEIICKGV
                     DEKGKVKEKKECFEKIKEIYTGLKEKNNG"
     gene            complement(21273..21395)
                     /locus_tag="PLF90_02555"
     CDS             complement(21273..21395)
                     /locus_tag="PLF90_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOK56199.1"
                     /translation="MCKSCGCGIKREKKKYVCKKCGKESEKQEICCGEKMVEKE"
     gene            complement(21409..22071)
                     /locus_tag="PLF90_02560"
     CDS             complement(21409..22071)
                     /locus_tag="PLF90_02560"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOK56200.1"
                     /translation="MEKISRMPLLGDEFPKMKVVTTHGIIDLPDYFKGKWFILFSHPG
                     DFTPVCTTEFVAFQNKYNKFRELNCELIGLSVDQVFAHIKWEEWIKEKFGVEIEFPII
                     ADTGEVAEILGLIHPGKGTNTVRAVFIVDSESKIRLILYYPQEVGRNIDEILRVLNAL
                     QTTDKYKVATPADWPNNEIIGSNVIIPPARDVKTAKERLEKSEKGEFKCYDWWFCYKK
                     LK"
     gene            complement(22091..22675)
                     /locus_tag="PLF90_02565"
                     /pseudo
     CDS             complement(22091..22675)
                     /locus_tag="PLF90_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045226.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
     gene            complement(22692..>22877)
                     /locus_tag="PLF90_02570"
     CDS             complement(22692..>22877)
                     /locus_tag="PLF90_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOK56201.1"
                     /translation="IAVAVRCSYCIGIHVEKAFKAGATEDEILEAGMVAVLMGGGPAY
                     TYLTDLVEAIKMVKEKI"
BASE COUNT         8027 a         3585 c         2885 g         8280 t
ORIGIN      
        1 aaatatgcgg tttttagttt taaaacttta ggaagaaaat acaaagaaga ataacatttt
       61 atgagaaccc ctttccagag agatagggat agaataattc attcaacagc attcagacgg
      121 cttgaatata aaacacaggt ttttatatat catgaaggtg actattacag aaacagatta
      181 acccataccc ttgaagttca gcaaattgca cgaaccatag caagagaact gaaggttaac
      241 gaggacttag ttgaagcaat atctcttgca cacgacctcg ggcatacacc ttttggtcat
      301 aaaggagaat ctgttttaaa tgaaataatg caattacatg gttttccggg ttttgaacat
      361 aatagacagg gtttgagaat agtggactat cttgaagaaa gatatccgga ttttccggga
      421 ttgaatttaa catatgaagt aagggaggga ataataaaac acatgacaag ttatgatatt
      481 ccactaatag gtaaagagtt tgaagaattt ataaaattta aatcccctac tattgaagca
      541 caaattgtaa atattgcaga tgaaattgct tatacctgtc atgaccttga tgacggtatt
      601 aaatccgaaa taataactta taaggatgta gaggatgttg aattatggaa agaaatagaa
      661 agaataagtg atttagataa acaaccggaa aaacataaaa gacatataat gataagaaat
      721 ctcataaact atcttgtagt agatttgatt gaagaaacaa agaaaaatct gtcaaaaata
      781 aatccacaat ctgttgaaga cgttagaaat tcagaaatta tagtgaaaaa ctctgaaaaa
      841 gttagaactc aacatgctga attgaaaaaa tttcttgaag aaaaaatgta taaacatcct
      901 aaagttataa ggatgacaga aaaaggagga agaattataa gggaactatt ttatgcttat
      961 tataatgaac caagacagtt gccctatcac attcaaaaga aaatagaaaa agaaccaaaa
     1021 gaaattgtta tttgtgacta tattgcaggt atgactgata gatttgcttt aagtgaatat
     1081 cagaaattat ttgaccctac ttctttttga aaatggatga aaaaatttac tggatagcat
     1141 taaatcttat cggaatttct tatgtaaaac tcaaaaaatt acttgaagaa cttaaaagag
     1201 ttgaagaaat tttcaaactg aatacaaaaa aattaataga acttggatta tcaaaaaaca
     1261 ttgcagaaaa aatagaaaga tgggaagaat taccgttaga agaagaaatt aaatatattg
     1321 aaaatgaagg aataaatatt ataacgatag acgataaaaa ttacccgagt ttacttaaag
     1381 aaatttatga tccaccattt ttaatttatt ttaaagggaa attagattta aatgctccct
     1441 caataagtat tgtgggaaca agaaatcctt ctgtttatgg tttaaaaatg gcggaaaaat
     1501 ttgcttttga acttgcttct tatggtttta taattgtaag tggtcttgca agaggaatag
     1561 atacttctgc tcatatagga gcaattagag caaatggaaa aacagtaggt gtaatgggtt
     1621 caggatttaa aaatttttat ccaccggaaa ataaaaaact tgaaaaagat atgataaaaa
     1681 atggagccat aataacagaa tttccatctt atactttacc ggaaaaatac aactttccta
     1741 aaagaaatag aataatttca ggcctttcaa aaggtttaat tgtaatagaa gcaggtccaa
     1801 aaagtggtgc tttaattact gcaaattttg ctcttgaaca gggaaaagat gtttttgctt
     1861 taccgggaag agttgacacc ctttattcta aaggaacaaa taaacttttg aaagaaggag
     1921 caattttaat tgaagatgtt aaggatatac ttgaaacaat gaatatagaa atagaaaata
     1981 tagaaaacaa aaaagagaaa attattttag aatcagaaga agaaaaaaat attttaagtg
     2041 tacttgaaca gacatcaagt attgaagaaa tacaaatgaa aacaaaaata gaaccaaata
     2101 ttttgttgtc tcttttaaca aatcttcaga taaaaggatt gattgaggaa ttaccaggaa
     2161 aactttatag gaaaaaataa atatttttaa ttaaatatct tgagttttca gaaataaaaa
     2221 aatattgtat aattttttct ttaaaaacag gagaaaaaaa tgaaatggag taaatacttt
     2281 atacccactc aaaaagaagc accaaaagag gcagaaacca ttagtcataa attattaata
     2341 agagcaggtt taatagataa acttgcttct ggagtttata catacttacc ccttggttta
     2401 aaagttttaa gaaaagttga aaatatagtc agggaagaga tgaacaactc aggagcaata
     2461 gaattactta tgccttcttt acaacctgct actttatgga aagaaacagg tcgtcttgat
     2521 aaaatgggaa aagatatgat taaatttatt gacaggcatg aaagggaaat gatttttggc
     2581 cctacacatg aagaagttat aactgacata gttaggagat acataaaatc ttggaaacag
     2641 ttacccataa ttttatacca aatccagaca aaattcagag acgaaataag accaaggttt
     2701 tcaataataa ggtcaagaga atttttaatg aaagatgcat acagttttga tattaatgaa
     2761 gagggacttg ataaatcata tattttaatg aaagaagcat ataaaaaaat atttgaaaga
     2821 tgtggactaa aatttactat cagagaagca gaaagtggcg ttataggagg aaaattttca
     2881 gaagaatttg tggcagatgg agaatgcagt gaacttgaag taggtcatat atttaaactc
     2941 ggacttgaat acagtaaatc tatgaaagca tattttgtgg atagagatgg aaaagaaaaa
     3001 ccaatatata tgggttgtta tggaatagga gtcagcagaa tagtcgccgc aataattgaa
     3061 ggaaattacg ataaagatgg cattatctgg cctatttctg tcgctccttt tctggctgat
     3121 attatattaa caaacaaaga agatgataaa atcgtaaata caagtgaaaa aatttataat
     3181 ggacttaagg aaaatggttt tgatgtcatt tttgatgata gagatgaaag tgcaggtgta
     3241 aaattcaagg atgcagacct ttgtggtttt ccatatcaaa ttataatagg taaaaaaata
     3301 aaagatgata aagtagaaat aaaagaaaga aaaacaggga aaataacaga gatcaatccg
     3361 gataaaattg ttgaatactt caaaacacta aaaaattgaa attttaatta atgaattttt
     3421 aacttcaaaa taaccttttt cgtaattgaa tatctttttt tataatttct acaacttcct
     3481 ccagagtaaa atatccaagc ataccactac catgctttct cactgaaagt ttattttctt
     3541 taatttcttt atcaccaata attatcataa aagggatttt ctcgtcttct gcttctttaa
     3601 tcttcttatt tattttttca tttctcaaat caactcctgt tctaaaatct tcttctaatt
     3661 ttttcttcaa attaagacag aattcgttat gcctgtccgc aatcggtaaa attcttatct
     3721 gttcaggggc aagccataaa ggaaaattac ctttataatg ttcaattaaa attccaaaaa
     3781 atctttccag agaaccaaga attgccctgt gtatcattat gggtgtttca aaatttccat
     3841 ccttatcaat atatttaatt tcaaattttt cagggatatt aaaatcaatt tggattgttg
     3901 aacactgcca ttctcttcca agacaatctt ttatttttat atcaattttt ggaccataaa
     3961 atacaccttc tccagggtct atttcataat ttatattctt tgtttttaaa gcgttttcaa
     4021 gtgcttttgt tgctctctcc cagttttcaa ctgttcccac aaatttttca ggccttgtag
     4081 acaaaaaaac tctgtaatcc ttaaatccaa aactgcttag aaaaaaatat acaagttcta
     4141 ttattttaca aacctcatct tcaagttgtt caggtgtaca aaaaatatga gcatcgtctt
     4201 gagtaaaacc tctaaccctc aataatccgt gtaaaacacc gggtttttca tatctgtaaa
     4261 cagttccaag ttcagcccat ctaataggca ggtccctgta acttctgatt ttactcatat
     4321 aaatctgtat atgaaaagga caattcattg gtttcaattg atacattcta ccattttcaa
     4381 tttccattct tggaaacata tattcactat aaaaagaaag atgtcctgat ttctcccata
     4441 aagaaatatc cgctatatgt ggagtataaa caagttcata accatttttt aaatgaacct
     4501 ctttccagaa atcttcaata atttttctta taattgctcc ttttggatgc caaaagataa
     4561 gaccgggtcc atattctgga tgaatactga aaaggtcgag ttttttcccg agaatcctat
     4621 ggtctctttc ctttgcctct tctataaatt tcaggaattc atcaagttct tctttagttt
     4681 caaaagatat cccatatatt ctctgcatac tctctctatt ttcgtctcct ctccaatagg
     4741 ttgacgaaac tgaaagcagt ttaaaaaatt taacctctcc tgtaaaattt aaatgaggcc
     4801 cttcacataa atctataaac tctccctgct tgtaaaaaga aacaatctca ttattaatat
     4861 ccttaagaat ttcaagttta aatggctcat tttttaaaat ttcttctgct tcttttttgt
     4921 tcttttcaaa tctttctatt tcaaaattct ctttaattat ctttctcatc tcttcttcaa
     4981 ttttttctaa atcttccgga gtaaatggtt tatccctgta aaaatcataa taaaaacctt
     5041 tatcaatggc aggaccggta cctaatttta cttcgggaaa taaccgttta acagcctgag
     5101 ccattatatg agaagttgta tgccagtatt ttttataatc cattttttat cctttggaaa
     5161 aaaatcgggg ccgacgggat tcgaacccgc gacctccaga tcgacagtct ggcattctgg
     5221 tccgctgaac tacggcccct gattaaactt tataaatttt acactttaaa ttttccaatg
     5281 tcaatttttc tataggtatt gacatttatt tattgtagat tataatatat gaatatatgg
     5341 tcatatgaga aaatgaaaat ggaaaaatat acagaaattt ttaagacagt tggagaaaaa
     5401 acaaggttga aaattgtgaa attacttttg aaatcagaag ttcctctatg tgtgtgtgaa
     5461 attatggatt gtttaaaaga aaatcaaaca aatatatcaa aacacttgaa aattttgaaa
     5521 tatgcaggaa ttgttgaaga aaaaagagaa ggtaaatttg ttatgtattc actcacaaaa
     5581 tcagaggata gatttttact atctttactc aataccatca attctatccc agaaaatata
     5641 tttaaagaag agattgaatg cctgaaaaaa agattaaatt taagagaaaa tggaaaatgt
     5701 gtaataggta taaggagaaa ataaaatgat aaaagaaatt attgttgggg gatttttagc
     5761 acttaaagat tatattgcaa ctcatgtttt aacctgtctt attcctgcat ttttacttgc
     5821 aggtggaatg gtaagtttta taaataaaga aataataata aattaccttg gagaaaaagt
     5881 caataaaata aagtcattct ctattgcagg tatttcaagt tttcttattg cagcgtgttc
     5941 ttgtacagta attcctgttg caagtggtct ttattataca ggcgctggaa ttggttctgc
     6001 ttttatagtt ctttgggttg ctccttcatc aaatatacta tctttaattt atacaggaac
     6061 tattatcgga ggccagatag tcatttcaag agttataagt gctttaataa tggcttttgt
     6121 agttggaagt gttatgagtt ttgttttcag aaaagaaaaa atagaagaga taaaattaga
     6181 aaaagaacca gaaaaaaaaa taattgaaag gaattactta attcttctga ttttaatact
     6241 tttatccctg cttgcaccaa actatattgt tcaaaaaggt ccttactgga aaaaagttat
     6301 tgtatggttt atctttaccc tttttgtggc aatttttgct tttaaaaaaa ttgaaaagga
     6361 aaaaattaaa gattggttaa gagaaacatt attttttgtc agaatgattt tccctctttt
     6421 actcatcggt gtttttatcg ttggaattat aggaaaaatt ttaccttcaa actggattga
     6481 gagatttctt ggtggaaata gtttaagaag cagttttttt gcaactttaa ttggagcaac
     6541 aacttatttt gcaactctaa cagaagcacc atttgttgac actttaatga aactcggaat
     6601 gggaaaaggt cctgctcttg ctttactttt aacaggtcct ggattgagtt tacctaactg
     6661 gatagcaatt tcaaaagttt ttggtgtaaa aaaagcaata gtttatgtta taacaattgt
     6721 tttttgtgga acatttgtag gatggttttt tggtaatttt atattgaaat aaaaaggagg
     6781 aagatatgga aattttaatt gttggtgctg gatgtccaaa atgtcaggca gtagaaaaag
     6841 ttgttagaga agttgtggaa gaattaaaaa ttgaagcaaa tgtttctcat ctttatgata
     6901 taaaagaatt tccaaaatat aaagttacaa taacgcctgc aatagtaatt gatggaaagg
     6961 tcgtttttgc cggtaaaatt ccgtcaaaag aagaagtcaa aaaattactt agaaaataaa
     7021 aaaggaggta ttaaaatgaa gccaagattt attatgtgta tctgtacagg acagtgtcct
     7081 gggtttaaaa gtttagattt atggtcttta ataaattttg taagaaatga attagatgtt
     7141 gagtatgcta tagttcatcc gcaactctgt gtagatgatg gagacagatt tattaaggat
     7201 tatataaaac cggatgttaa gtatgttatt ggtgcgtgtg accctaagat gcagagaaaa
     7261 atgttcaaag atgcttttat ggaagcaggt ggtgattatg ataaacaggt tatttctctt
     7321 gatttaagaa atatgccaac agaagaagca atgaaaaaag ttaaggaagc agttgaaagc
     7381 ataaaatgaa aatacaaaaa aggagaaaaa tatatggaga aagatagaag tacatatact
     7441 ggactgccaa gaaaaaaaat tgactggttt cccaaaattg attattcaaa atgtaaaccc
     7501 gaagaatgcg gttatcattg tgtaaatttc tgtccattta aagtattttc aaaaaatgaa
     7561 aatgaccaaa aagttattgt gtctcagccc tatgaatgta atgtcggaga tgaatcttgt
     7621 aaatttcagt gtccatttgg agcaataagt tttccaacaa gagaagagtt aaaaatgatg
     7681 ttaaaaaaag ttagagaaaa atatgaaaaa cagggaggga aaaatgttta aaaatatacc
     7741 aagagaaaag ataccatggt ttcctactgt tgattatgaa aaatgtattg gttgtaagga
     7801 atgctataac ttctgtaaaa atggtgttta tgaatgggat gaagaaaaga ataaacccat
     7861 agttaaaaat ccatacaatt gcgtggttgg atgttctgcc tgtgcaaacc tatgtaatgg
     7921 agaagcaatc agttttccga aaatgtcaga tataaaaaaa atgttagaaa aggagaaata
     7981 attatgatat ggaaaatata agttatattg gcagttttat acttggaatt attacatgtt
     8041 tatcaccctg ctctattgcg atattaattg ctgttttgtc ttttataata agcgaggaag
     8101 aaagtataaa aaacggaata tatataggga ttttttttac cctcggtatg tctcttgtat
     8161 ttttcatact tggtatattt atctctaaaa ttggacaact tttaaaattt tctcattatt
     8221 tttatttaat ctcaggaatc cttctgatat tttttggact tctaaatctt ggtattttta
     8281 aaaaaaagaa aacatcaggg tcattgaatt tttttcagag atttggtttt tcaattttat
     8341 ctttaaacaa atattcaaaa cttttaacat catttttatt tggaatactt ttctctctcg
     8401 gatgggctcc ctgtgctact tctttaatta tgcctgttgc tattttagtt atggctcagg
     8461 aaataagtat attaaaaggt ggatttttgc tttttctttt tggactcgga catggatttc
     8521 ctgttattcc tctttctgct ttatccggta cggtaaggac aaacataaca aaaaagacag
     8581 caagaaaagg cgatttgatt ataaagatat tcggaattat tataattttg tttggaattc
     8641 tttttatagt ttatggacct aaaattaata cattaattgg aggcaaaaaa tgaaaaaact
     8701 aattatactt tttactcttt tgtttctctt ctcttttgca aaaactaata aagcacctga
     8761 ttttaattta aaagaccctt atggtgaaac tttttctctt tctcaattca aaggtaaaat
     8821 tgttgttctt aatttcttca aaatttattg tggcggaaga actgctccag gtactttaaa
     8881 ccagattgaa gaactcaaaa aaatatgtaa tgaaatgtgt aagggcaaaa aatgtaaaga
     8941 cggaaattta acaattattg gcataactct ttcaagttgt tcaacaacag atttgaaaat
     9001 ctgggttgaa gaaaagggaa taaaatggta tgtaggaaat gaccttgatg actataatct
     9061 tgatataatt aaaaattatg cagaatattt gaaaacattg aaagacccgt gtttaatctt
     9121 tattaacaaa aatggtgaag ttgtgtttaa atcaaattat atgaaagctg atgaaataaa
     9181 gaaaaaatta aaagaactca atttaatatg agaaaagagg ttattgaatt tgaagaaaaa
     9241 gatatagttt atattaccag aattgttctt gataataaca aagaggaagc gctgaaattt
     9301 ttgaaaaaga tttacatcgt tctcaaaaaa agagaaaaat cccactgtaa aacaagttat
     9361 gcatgggaaa aagatattga aaaaaatcag aaatgaatat aacaattgaa aaaatattac
     9421 catgtatcgc tgacccggaa aaaatcagat ttattgcttc tattgatgaa gatatatcag
     9481 agattcttcc atacttaaat gtagttttac caaaagcaat atataaccac gaaggaaaat
     9541 gtttaacaat tacaaatgaa gaagggatta ttacaattta cccccataaa atatctgctg
     9601 ggaaaattaa ggatgaaaat ggtgcgagag aaataataaa gtggttaaaa gaagaaattg
     9661 aatactgtat taaaaataaa gataaaatta aacctgatta tacaagaaaa gataaacttg
     9721 aggtattaca tgtctataaa ttacttcctc aaaaaaactg taaaaaatgt ggtgaaaatt
     9781 cctgtcttgc tttttctttt aaagttataa atgaagaaaa aaacattatt aactgtaaag
     9841 aactgtttct accggaatat aacgaaaaaa gaaaactatt atttgatatg ttaaaagaat
     9901 ctggctataa agtccctgat ttttaatatt tactttataa aagtatcaag aatcatcata
     9961 attaaaaacc cgattataaa aaaaatcgtt gaaatatcag tattttctgc tttttgagtt
    10021 tctggaatga cttcttcaat tattacataa atcatagcac ctgcggcaaa agataaagca
    10081 aaaggtaaaa ttttaaaata gattgaagtg agtaaagcac caatgatagc aaaaattggt
    10141 tcaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatttta aaatagattg
    10261 aagtgagtaa agcaccaatg atagcaaaaa ttggttcaac aattcctgaa agttgaccat
    10321 aaaaaaaact ttttctttta ctcaatcctg atgctaaaag aggtaaactc actgcaagtc
    10381 cctcagggaa attttgaata ccaataccaa aagcaagtga aatagaagaa gaaaatgttt
    10441 ccttactttt taagtatttc aaagaaccaa aagacactcc aacagaaata ccttctggaa
    10501 tattatgaat tacaattgct aaaacaaaca aaaaataaac cggtaattgc tttcttatcc
    10561 cctctttttc tgaaatattt gtatttaaat gtaaatgggg aatcaattta tcaagtccaa
    10621 aaagaaaaaa actacccaat aaaaatccaa ttaaaactgg aaaccattga gggatattta
    10681 aattttttga atattctata gatggaagaa taagagaaaa aaaactcgca gaaagcatta
    10741 tcccaccagc aaaaccaagc atacagtcaa aaaattttct attcggtttt ctattaaaca
    10801 atacaataga tgcaccgagc aaagtaataa accaggtaaa aagaccacca taaaaggact
    10861 gaagcaaagg agtaaagtta taaaatttat ttaacattac atattcatcc agttccagat
    10921 tttactttct tccttttcaa accaatcgcc tgtttcagtc aaataagagt atacatttat
    10981 aattgcctca atatgtgatt tttcttgctc cattaagaat ttaaataatc ttttctcttt
    11041 ttcatttttt acttcattat aaaacttttc atattgtaaa tatccctttt tttctatttc
    11101 gagtgccatt ttatatccat ctatattgct ttcaatttta tttttctctt taagtgaggt
    11161 aaaaaactct ttgagatcct cctcaatcat ttcagttttt tcagttttat aatcatcact
    11221 taatacaaaa ttttcaataa tttcaagatg ctcaatttcc tgttttgcaa gtgaataaaa
    11281 aagttttttt gttattaaat tttctgactt cataccaatt tcaatataca aatttgctcc
    11341 atgcttttca aattcctttg caaactttat tccattttcc ataaaacctc cttttaaatc
    11401 tcacttatac ttgtatcagg accatttgga attttaacaa atattctcaa acctttctca
    11461 ataatctcca tcttttctaa atcaaaatca tctggtaaaa tccttatatg agaaatattt
    11521 tctcgtggta taagtataat ccttttctct ttatcgatat ctacaggact tacctggcct
    11581 tttataacta caataccttt cccctcaatc ataatccagt tttctgtaaa tttcaaaacc
    11641 ctccctatga aaacccacaa tttttgttca agataatatt ttttaaacct tatttttaca
    11701 atcttccctt ctatcatttt tcctccttgc ttataaaatc atcaattttt ctttttaaaa
    11761 tatctcttac ttttctaaat ccttctatct catttttttc agcagggtca ggtatctccc
    11821 atgctattgt tttctttgtt tttcctggga aaaatggaca taatcccttt ttatcaccta
    11881 cacaaacagt tataacataa tcaaattctt cattcagaaa ctcttttatt gatttacttc
    11941 tatgatttga tatatctata cctatttctt tcattacttc aattgcaagt ggatgaacaa
    12001 atttaacaat agaacctgca ctaaatgcaa taaaattatc tttataaaaa taattcacaa
    12061 tcccttctgc tatttgactt cttgctgaat tttccctaca cagaaataat atttttttca
    12121 tttgttttcc tcaatatgat gcttaattat tttaccttct gtcataaaaa taacatcctc
    12181 gcttatattt gttgaaaggt ctgctattct ttcaagattt tgtgcaatcc ttataagatg
    12241 taaagacctt tcaattgtag tataatcagc tatcatatat gttataagtt cccttgtaat
    12301 ttgatctttt agattatcta caatatcatc tcttttacac acatctcttg caagtttctc
    12361 attttcttca ataaatgaat ttatactatc tttcaccatt tttattgttt catctgccat
    12421 ccttggaata tcaatcaatg gtttcacatc aggtttttca attaaaaata atccactttc
    12481 agaaatattt acagcatggt ctcctattct ttctaaatca ttatttatct ttaaaatcat
    12541 caatacaatc cttaaatttt ttgctttcgg ttcatattga gcaattattt ctgcacacaa
    12601 ctcatcaatt tcaatctcaa atttatttgc gattggttca agtttttcta tcacttcttt
    12661 taaatctatt ccatttttat ttattattcc ttttatactt ttatcaatca ttttctctat
    12721 cagcaatgaa aattcaataa ttttttgttt taaaatcttt aatttatcct ctaacatatt
    12781 cacccccaaa attaaccaaa cttaccactt aaatattcct ctgttattgg attttcagga
    12841 gcagtaaaaa ttttttcact tttaccaaat tcaattaact ctccaagata aagaaaagca
    12901 acataatcag caattcttga tgcctgcgaa atattatgag tgactataat tatagtaaca
    12961 ttatttttaa gtttttctaa aagattttca atttcaatag ttgatttagg atcaagagaa
    13021 gatgttggtt catcaagtaa tataatttct ggatttaatg caagaacacg tgctatacat
    13081 aatctttgtt gttgccctcc tgaaaattga gttccattta aatacaattt atctttgact
    13141 tcttcccata atgcaacttt ttttaatgtc tcttccacaa ttctatccct ttcctctttt
    13201 cttaatttta tcccatttat tatataacct gcaaggacat tttcataaat agttaaatga
    13261 gggaaaggat ttggtttctg aaaaaccata ccaattcttc ttcgcaataa aacagtattc
    13321 atttcaaaaa tattttttcc atctaaataa atattacctt tatgaaacgc atctttattt
    13381 aactcatgca ttctatttat acatctaatc aatgttgtct tgccacatcc tgaaggtccc
    13441 ataattgcag tgattttatt tttaagaatt ccaagattaa tatctttaat cgctattgaa
    13501 tttccatatc ctgccgaaaa atcttcaatc tttataattt cactttccat cttctaaatc
    13561 ctattttact tatagaattc aaaaataaaa caataattac aagtattaaa gcacatcccc
    13621 atgcatgttt atgccagttt tcataaggac tcattgtata tacaaaaatt aaatgaggta
    13681 aagaacttac tggttttaaa atattaaaat taagaaaagg attaccaaaa gcagtaaaaa
    13741 gcaatggagc agtttctcca gcaattcttc ctataccagt taaaattcct gatataatac
    13801 catttaaaga acaagggagt ataactttta aaattgtcct cggatatgta gcaccaagtg
    13861 atagagaaga ttctattagc gaaaatggta tcatttgaag actttctttt gtggatttta
    13921 taataatagg taaaaagatt aaagataaag caattgctcc agatattgcg gaaaagtggt
    13981 ccatttgttt tactatccat aaataagcaa taattcctat aacaattgat ggtattcctt
    14041 gaagaacatc tattaaagta ttacaaaatt ctccaaattt tttatcactg aattcataga
    14101 gaaaaagacc aaggaaaata cctgttggta ttgaaaaaaa agatgctaaa aaaattaaaa
    14161 tgaaactacc gacaattccg tttaaaatcc ctcctcctat ttctccaggc ggtttcggta
    14221 atgaagttaa aaattcccag ttaattgcgg taaatccttt tttcaaaatt gtaaaaaaga
    14281 aaaataaaag tggtacaatt gaaaggaaag aaaaaaatat cacaaaaaat ctaaaaattt
    14341 tatccttaaa aattttcata tcttcaccct cattcttttt attattatat ttgcgacaaa
    14401 attcattata aatgtgatta aaaacaaaat taaagcaagt tctatcagtg aagagatatg
    14461 aatattctca accgcctcta aaaattcatt tgcaattaca gaagatattg tatttccagg
    14521 ggaaaaaata tttttaggta tataatttga atttcctata accattgtaa cagccattgt
    14581 ttcacctatt gccctaccaa aagaaagtaa aaaacctgcc aaaataccac ttcttgcata
    14641 tggaaatacg atttttttta caacctcata actggttgct ccaaaagaaa atcccgcttc
    14701 ttttaaatca tttggaacaa gctttataac ttctttaccc acagaagttg catatggaag
    14761 aatcataatt gaaagagtta aagatgcagt aaaaatacat actcctgttg gaggcaatga
    14821 aagttttgcc tggatattcc ttataattgg agtaagataa aaaagtgccc ataaaccaat
    14881 aattactgat ggaataacag caagaattcc tataagtgtt tcaagtattt ttgataaaaa
    14941 ggtatctcta taaaactctc caacaaaaag agaaacagaa agagaaaatg ggatagaaat
    15001 tataagagaa attatagatg tcaataaagt tcctattata aaaggtaaag caccgaaatt
    15061 tccaaaaaca gggtcccatg tcttttcaag aaaaaattta aaaccaaaac ttttaataga
    15121 tggtaatgaa ttgataaaaa gagtaaaaaa aatagatagg aggagtccga taataaggac
    15181 tcctcctatt attccaagta aagttttata ggctttttct gatgtcattt aatcaatatt
    15241 ttactatcat aatgaagttt tttcaacaaa ttttctgcct tttttactgc ttctttacca
    15301 agtggagcat aatttaaagg ttttgtatat ttctgacctt catgagtcat ccaccagagc
    15361 aactttacta aattttctgc cttttcataa ctcatttttt cattcgcatt ttttaaattt
    15421 ttataaacaa gtatataagt aaatgctgat attggatatc catttttatt ttctgtatct
    15481 gtaattgaaa ctctcatatc atctggtaat tttatatctg atgcaaatgt tattgattca
    15541 atagatggtt ttacaaaatt tccagattta tttttaataa ttccaaaagg tagattattc
    15601 tgcaaagcat atgtaagttc aacatatcca attgcacctg gtgttccttt aacaattcct
    15661 gaaacaccct cattaccttt acctccaatt ccaactggcc aattaacact tgttcctcta
    15721 ccaacttttg ttttccattc ttcactaact ttagaaagat aatcagtaaa aacaaaagtt
    15781 gttccactac catcagacct tcttacaact attattggca tatcaggaag attaacattt
    15841 gaatttacct tttttatctt atcatcattc cattttgtaa ttttaccaag gtaaatatct
    15901 gaaattatat caggtgtaaa tttaagttct ttttctacag gaagattata tgttataaca
    15961 actgcaccaa gacatgttgg aatatgtaaa atttcatcat caaaatcttt aagttgctca
    16021 tctgataaaa ttgcgtcact tccagcaaaa tcaactgtct ttgcctttaa ttgtcttatc
    16081 cctcctcctg aacctattga ttgataattt atttttactc cataaatttt ataatactca
    16141 tcaaacattt tagaataaag aggatatgga aaagttgctc ctgcccccac taattctgtc
    16201 tgagattcaa cttttgcaat tcctacaaaa agcattacta atcccaaaaa tatttttttt
    16261 atcatctttt cctccttttt aaaattttac taaataatca atttgaagtg tatttctatt
    16321 atctgttcta tttacaccaa gtgatttacc tatgaaatat gttataccaa atgatgaatt
    16381 tttgttaact tgataaacaa atccaatctt atgtcctttt aaatcagttc ctcctccatt
    16441 tatatctgaa tctgagaata tcgccaatgt agcatctgca tcaatttttc tgtaattata
    16501 ttcaaacttc cagtcattct tttcttttaa ttttcctata gaaaatccaa taagatatcc
    16561 tttatcctct ctaactccac tttcaatatt ttttaagtaa tcagcataca atgtaaatgg
    16621 tttttctgca attttaaatg gtgttaattc taatataata tcaaatggtt tatattcata
    16681 tatatattta ccattacttt caagtgtatt accctttggc tcatattttg gtgaaatttc
    16741 tgattgtttt ttccctttta aactttttgt ggtataaata gaagtagcaa ttttaaattc
    16801 tacttcattt ccaattttac taccatatcc caattgaatt ccatataaaa aggggtcatc
    16861 tgttaaattt ttaaattcag ccaaaggaaa aattccagaa ttaataaata aacctttttg
    16921 atttttgtag ataaaagcaa gtccttcagg atttatatct gaatcccaga taatatctgt
    16981 tgtataaaat ggattttcaa ttttacctgc aattaaacca aaatcattaa aattgtgttt
    17041 caaatatgct ctatccaacc aaaaactttt ttgactgaat gcttcgccaa atgtttgatt
    17101 ggttgaagtt tgttcaccag ttcctgttgc caatcttaat cctatttcag tattttcaga
    17161 aatctttgtt ttaaatcccc atcttgccct tattctatat ctatctctat caggactccc
    17221 ttctatatct tcattttgat atctaactct tatgtcacct gaaaattttg tatttttaag
    17281 aaaatcgtat ttctctttct ccttttcttt ctctttcttt atttcatttt ttatttgctc
    17341 tgcctcgtct ttactaatta ttcctttctc aatcaacttt ttcattaaaa tgtcaacctc
    17401 tcctgcataa gaaaaattaa atatcaaaaa caaaaataaa aaaattatat aaaatttcct
    17461 cattctttcc ctccttttta tttaaagttt taaagaaata tctcaataaa agatttttac
    17521 tctcatattt catcacctcc tttcttttta tttctctatc catttttttt ccttccattt
    17581 taattttcct cttctttaca attatttact ttttttttgt taaatccatt taaagtattt
    17641 gttaattttc tgttaaaatt ctttaacttc attaatgctt tttgcttaat ctgtcttacc
    17701 ttttcccttt ttatttttaa ctctttggct atttcttcaa gtgtcttccc ttccaaataa
    17761 aatttttcaa ttatatttct ttcaaaaggt gatagaattt ccagatttag tttataagaa
    17821 atttctgaaa attttgtaat atttgtatct gtggtaaatg aaagagcatc ttttgaaata
    17881 tatatttttt gcttcttgta tttttctaca ctttcaagaa tatatttttt tatccagtag
    17941 gttgcataag ttaaaaaatt cacattcatt tcttctttaa aattattttt tgcttcaata
    18001 agtcctatac aaccttcttg aaaaaggtct tcactttcta caccaattcc ctcatatctt
    18061 ttcacaattt ttttaacaag cggaaaataa ttttcaattt ttatttccat tcataaaata
    18121 agatacaagg aaaaaattaa gaaagtttaa aatttttgtt aaaattctgt taaaaattaa
    18181 actggaaggt atactttaaa ttttgttcct ttatttaact cactttttac ttctattttt
    18241 ccattatgga ggtcaactat ctttttcaca attgaaagtc caagacctgt ccctgctttt
    18301 ggtttacttt ttgagccaat aaaaaattca tcaaatattt taggaaggtc ttcttttgaa
    18361 attccatacc cagtatcaga aacttcaaaa attacataat ttttttcctt atacaaatta
    18421 atttctattt tccctttatc tgtaaattta actgcattgt ctataagatt gattattact
    18481 tgctcaattt taaactcatc acccttaatc aatggcacat ctgaaaaatt taatacaaac
    18541 gataatccct tcttttctac tttttcttta tacaaatcat aaactttttt aacaacttta
    18601 agtaaatcaa attcttccaa ttctattttt tttgattcaa gagaagatag attaagaata
    18661 ttttctaaaa tttttatcaa tctatctatc tggtttttta taataatggc ataattttta
    18721 atatttgaat ctcctgtctc atcttctatg gtttcagcat atcctttaat aactgttagc
    18781 ggtgttttca attcatgaga tatgttaaat ataaaatatt ttcttatttt ctcaatttct
    18841 tttgttgtag ttatatcaaa cataaataaa attatttttt ctcctaaata gattgttttt
    18901 aaaagatacc atctattatc tatctctgcc tctttttcct gtcctttttt gtaattcatt
    18961 gtcttttcaa taatttcatt tatttcaaaa ttttctataa cctcccagaa atacctattt
    19021 tgaaaaggag atttcaaaaa ttttttaaaa ttaacattga aatttaaaag ttttccttct
    19081 tcattaaaaa aagcaactgg ctcttccaga aattttataa cttcaaacat ctctacattc
    19141 tttttctcta attctgaagt aagcagtaat ttttcactta ttaaattcaa tttttcagca
    19201 atatttttta gttccttctg tttaaaaccc aataattttt ctccatattt ccctttacca
    19261 atcttttcaa taaactcatc aatcaatgaa aattctttta agaaaaatgg tatcataaaa
    19321 gtaaaaatgg aaataaaaat caagtatgtt attagaaaaa ttaaaccgaa ttgtttttta
    19381 aattcataaa aaattttttg aatatctgtt aaatttttac ttactctcaa aacaacttct
    19441 ttctctttta caaaaactgc ataataaaac atatactcat ttaaagtagg acttaatctt
    19501 atagattttc cttctccctt ttctattgca gatattattt caggtctatc tttgtgattt
    19561 tccataaatt ttggattttc ccttgtatca attattacag ttccatcttt atctatcaaa
    19621 gtaatcctaa ttcctgtata tttatcaatt ttctttaaat cttcaataaa tttcttattc
    19681 tctgaatttt tttctaaatc attataaatt ttaaaggcaa attctttaag attttcttca
    19741 acattaagaa aaactatttt atgaagggtt tgaataaaaa ttatataaaa taggataaaa
    19801 actgatccag aaaccaaaat agaagtccta acaaattttt taataagata ttttttcatt
    19861 cttcaatttt ataaccaatc cccctaacat tcacaataaa cctaccccct tcttttaatt
    19921 tttctcttaa tttttttata tgaacatcaa ctgttctatc aatgactgcc ttttcaccct
    19981 gccatagttc ttcaaggatt ttttctctgc taaacaccca gcctttttta ctaagtagaa
    20041 tttttaaaat gttaaattct gttgttgtaa gatttatttt ttctccttta acataaacct
    20101 catatttatt taaatcaact cttacaattc ctcctatgtc aataatatct gattttgttt
    20161 ggtatctcct taaaactgct ttaacccttg ctattaattc tttaattgaa aagggctttg
    20221 aaatataatc atctgctcca agttcaagac ccaaaacttt atccgtttct tctccctttg
    20281 cagttaaaat tatcaatgga atatctaaat attttctttc tctttttaaa tatttacata
    20341 tttcaagtcc atccatatca ggtagcatta aatcaagtat aatcaaatct ggtctatcta
    20401 atttcaaata attcagaaaa tcatttccat tttcaaaacc tttaatatta aaacctattt
    20461 tctcaaggtt atgttttaga agttgaagta tatctttttc atcttcaata attgctatcg
    20521 tctttttcat aagtatttaa gatattcaaa ggcagaaata tttgattttg caccttctcc
    20581 acaagcaaca attatctgtt ttgcaaatac atttgtaaaa aataatttct ctatcaataa
    20641 cctatacata gaacttttat ccattcccaa taaagacgtg tctttgtaga atatacaata
    20701 actttcttaa ccattatttt tttcttttaa acccgtataa atttctttta ttttctcaaa
    20761 acattctttt ttctctttca ctttaccttt ttcatcaacc cctttacata ttatttctcc
    20821 ataataactt gcaccaatcg ttgcaaaaaa atttttaatt atacttcttg cattatcaat
    20881 aaaatcatct ctattacttg cctcaacaca aataaaaatt ccttttttcc tttttggttt
    20941 aaatgactta aaaaaattta taccaagcca ttgacattga aacctatcta tcatcatctt
    21001 tgtttgagca gaaatagaac caaaaaagat aggggaagta acaacaacta tatctgaatt
    21061 ttctatctca tcataaacat tctgcatatc atcatttatc ttacatctac catcttttct
    21121 tatattttca cattcctgac atgggacaaa ttttaataaa ttcagatata tttttctaac
    21181 ttctgctttt tctttaaaat aactcaaaaa ttcatcaagt aatatttctg aatttccatt
    21241 tttcctcgga ctaccacata tccctattac tttcattcct tttctaccat cttttcacca
    21301 cagcatattt cctgtttttc tgactctttc ccacactttt tacatacata tttctttttt
    21361 tctcttttta ttccacagcc acaacttttg cacatatctc cctccttttt atttcaattt
    21421 tttataacaa aaccaccagt cataacattt aaattcgccc ttttcacttt tttccaatct
    21481 ctcttttgcg gtttttacat cccttgcagg aggaattata acatttgagc caataatttc
    21541 attatttggc cagtcagcag gagttgcaac tttatattta tctgttgtct gtaatgcatt
    21601 taaaaccctc aaaatttcat ctatatttct tcctacttct tgaggataat ataaaattaa
    21661 tctgatttta ctttctgaat caacaataaa aacagctctt actgtatttg ttccttttcc
    21721 aggatgaatt aaaccaagaa tttcggcaac ttctcctgta tctgctataa ttggaaactc
    21781 tatttctact ccaaactttt cttttatcca ttcttcccat ttaatatgtg caaaaacctg
    21841 gtcaacactt aaaccgataa gttcacaatt taattctcta aatttattat atttgttctg
    21901 aaaagcaaca aattccgtag tacaaacagg agtaaaatct cctggatgac taaataaaat
    21961 aaaccacttt cctttgaaat aatcaggtaa atcaataatt ccatgcgttg ttacgacttt
    22021 catttttgga aattcatcac ccaaaagagg cattctacta attttttcca tatttccctc
    22081 ctttttattt ttaatactct tcacatttta attgataaaa acttctatca tgactgcagg
    22141 aaggacattt ttcaggtggt gtgttcccaa aatgaacata tccacattcc ctgcataccc
    22201 accatacttc tttttctttt ttaaatactg tccctgcttc aacttctttt aaaatctttt
    22261 taaatctttc ttcatgatgt ttttctgcca cagcaattgc tcttaatctt tcagcaattt
    22321 ctggaaatcc ttccttttct gcaatatcag caaactcagg atacatcttt gtatattcat
    22381 aattttcacc tgaaatagcc gattttaaat tttcaatagt tgttcccaga gttgttaaaa
    22441 cctctgcttc aactttaatc acatcaaggg cttcattgct tttttgtttt aattcattaa
    22501 tcaatctgaa caaccatgtt gcgtgttctc tttcattatc cgctgtaata agaaaaattt
    22561 cacctatctg ctcgtatccc tattttctgg ctatttttga atagaaagtg tatctatttc
    22621 tcgcctgaca ttctccaaca aaagcttttg ccaagttttc aatcgttttt ttcatacctc
    22681 tccctccttt tttaaatttt ttcttttacc atttttattg cttcaactaa atctgttaaa
    22741 tatgtataag caggacctcc acccatcaaa acagcaacca ttcctgcctc aagaatttca
    22801 tcttctgttg ctcctgcttt gaatgctttt tcaacatgaa tacctataca gtaagaacat
    22861 ctaacagcaa ctgcaat
//
LOCUS       DAOSAT010000017        21924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_1697_length_21924_cov_5.714848, whole genome shotgun sequence.
ACCESSION   DAOSAT010000017 DAOSAT010000000
VERSION     DAOSAT010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21924
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1697_length_21924_cov_5.714848"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..770
                     /locus_tag="PLF90_02575"
     CDS             198..770
                     /locus_tag="PLF90_02575"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOK56202.1"
                     /translation="MENKRKLIIKVAEKLISQKGYSKTTIEEITRKAGIGKGTFYLYF
                     KNKNDLFFSIIKEEFENLMLKIKKEVEGIDDFFERLKKEIEMYLDYHEKNYAFFKILL
                     QEKPFIRKKSFEKFWKDFFERWDNFMKKGFEEQIKKNKIKKFDLEDIIYSLIGILHGN
                     IHRWILNERKYSLVKKKEVIFKIFIEGIRR"
     gene            773..2050
                     /locus_tag="PLF90_02580"
     CDS             773..2050
                     /locus_tag="PLF90_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOK56203.1"
                     /translation="MKKFLIFFFVFVLISFSEEVFNLDECIKIAIENNLKLKISQDKI
                     DSAYYQKEIAGKYKLPTFSTSFNYTHLGDNEGFVFNGIPMKFTEDNIFSLKFTLSQPI
                     FTGWKIEKTYEISKENYEKTKIDFENELSNLIFDVEKAYYNILKIKKNLETGLKYKEN
                     LEKHLSDAKKLFQEGIVTKLDILKTEVALKNAETRIIEIENYLKTAKANLNFLLGKSP
                     DHEFEIEDILEVKEEKKDYQWWKEMSLKERSEIKSMEKLISIYEKNIDIEKSNLYPQI
                     YFFFNYNIERGSQTSVGKWDTNWNTGFLLSYDIWNWGQTKDKIRKAEIEKEEIKKQFE
                     LIKNSIEIEVKNAYLNFLLAENKIEESKKQIELAEENFRVANLLYNEGLATNTDVIDA
                     ITALTDAKNNYYNSLYEYKISYSQLLKASGILK"
     gene            2059..3171
                     /locus_tag="PLF90_02585"
     CDS             2059..3171
                     /locus_tag="PLF90_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOK56204.1"
                     /translation="MKKIIWVLIVLIIILVVFNQFNKKKKEKKVEIVSETEIPVEVEK
                     VSEIVLKDEVECIGEIEPYQKVVVYSEISGQIEKLSVKEGDFVKKGDFIVQIDYKKRK
                     IDYENMENQIKAMEANLENIKKDYERFTKLYKEGVISEKKLDDIKTNYEVVSHQLESL
                     KTQFELVKIRLNEAFIYSPITGFIAEKFIDEGELITESSMMRSVPIVAIVDISKVKVK
                     LPVSSEEIGKVKTGQSVLIKLDAYPTKDFYGKVSKIYPVADEKTRTVDVEVIVENKNY
                     LIKPGMYCKGIIITGERKVLAIPLDAVMKLPASGNYYCFKVKDNTAEKVYIKVGKIKE
                     NLIEVKEGLNRDDIIITTSQGILDTGKKIKIIGEKK"
     gene            3168..6245
                     /locus_tag="PLF90_02590"
     CDS             3168..6245
                     /locus_tag="PLF90_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOK56205.1"
                     /translation="MNLPEFSVKRPYTTLMIFLAILIVSFVTIPKIPVDLMPEIEVPA
                     VSVIVPYPGASSSDVENDVVKYLEDYLTGVEDLDELISVSKDNVGIVTCKFKWGKNLD
                     TAVNDVRDKVDFAKKDIYQHAPDAEEPMIFKFSTSMTPVLIFSISSKTLWGDLYYIAD
                     KQIADQLKRAEGVGTVMVFGGIRRQINIYLDWEKINGYRIPPNLIVQRLAEENIDFPL
                     GEIKRGKRNYFLRTEGRYVNPEEIKDILITTIEGRPVYLRDIAKVDDYYEEVKSKAYT
                     DGEETVILMVQKQSGKNTVIVSNNVKRKIEEIKKFLPKDIKINTVFDTSEMILKSIKN
                     LINTVITAGILVILVTVFLLRRLKLSFIVSISIPFSLICAFLFLYIGGFTINLISLMS
                     LALVIGMVVDNSIVILENIVRHYEEGEKIDEASIFGASEVGTAIIASTITTIVVFVPL
                     IFTKGFIGIIFKQLAYIISTTIFMSLVVALLLVPMLSSKIIKGHPKETKFYKIGEKFL
                     TKIDDVYTNLLTWCLKHKNLFLSLIIIVFFASLLLFKFIGVEFFPEADNARIETTILM
                     NPSTRIEETDKIVREIGKIYEEEIPERKAWYGSCGESGSGIGAAMGRDEGPYAGSISA
                     RLTWREERKRSDKEIAEIIRKRIKEIPGIEKFSVSIASPLQLMFMGGAKQIEVELKGS
                     DLNKIVKYSEMLKERIEKIPGAVNVDTSYKKDRIEIHLKIDKEKAKNFGISTSSISQI
                     VRSYIYGYTATKYREGGEDFDVFVQLEEKQRYDIEKIEELPLFTPSGRVIKLKDVAEI
                     DFELGPVQIDRKNRQRIVKVRCDTFKRPMSEVLKDIEKEVEKLNIPEDITVSYGGMVK
                     QQRESFADLRLLLILGVILVYMVMVGQFESFKTPFIIFFSIPFTFIGSILFLLITGVN
                     FSIASFMAIIMLMGVVVNNAIVLVDYINILRARGYQLFDAITKGARNRIRPIMMTSLT
                     TIFGMIPMALGRGTGGEIWQSFGVPAIGGFLVSWIITLLLVPIIYLIMNKESK"
     gene            6242..6454
                     /locus_tag="PLF90_02595"
     CDS             6242..6454
                     /locus_tag="PLF90_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56206.1"
                     /translation="MKMFLIVSNIGIHEEILDILKECKVEEYTRIEKVQGVGKTAGPH
                     LGTNVWPSWNTLFFAQLLRMKRKVYY"
     gene            6549..7067
                     /locus_tag="PLF90_02600"
     CDS             6549..7067
                     /locus_tag="PLF90_02600"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOK56207.1"
                     /translation="MKKILFLIFLIFSSGFCEIRNFKVDFYTGYGFAKIQNPKNYEFY
                     TLMVDFTRPIKNSKNFFYQIEPYVSYVFSPADNFEIGVSGFLLYKFSDSNFQPYLKVG
                     TGIIYLSSDFVEQSTHFNFASSVSFGVRVFLKKVFLNTEIRLRHVSNAGIKLPNEGIN
                     SKLFLIGIGSDF"
     gene            7069..7950
                     /locus_tag="PLF90_02605"
     CDS             7069..7950
                     /locus_tag="PLF90_02605"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK56208.1"
                     /translation="MEKVFIDVHCHVFKRPYIWIDGKSLFSSPEQVIKRYDELGIEKG
                     VLLPIVNPEVYLPQSNEEIIEICEKYPGRFIPFCNIDPRAITNSPEADFEKILNYYKS
                     LGCKGIGEVMPNLPFLDERVLNLFRHAEKVGFPIIFDGNGKLGNTYGLYDEPGLPQLE
                     KCLSLFPDLKFIGHGPSFWSEISVLKDEEDRYGYPDYPVIEEGIVPVLFRKYRNLYGD
                     LSARSGYNALKRDTEYAVKFLSEFSDRLLFGTDLCRPDQDTPLIKFLKGLVEKGKISE
                     NIFRKIVRENAIKLLNL"
     gene            7964..8836
                     /locus_tag="PLF90_02610"
     CDS             7964..8836
                     /locus_tag="PLF90_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013303704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56209.1"
                     /translation="MKIKKFEMGEFIIGIPEDIGPRIIHFSLKKYPDRNVFEVIPDLT
                     IDTEEGKWQIYGGHRLWTSPEDKPRSYSLDNKPVKIEEKSKNISISGNAEIQNSVKKE
                     IEIKKTKEGIEVNHRIRNIGRWPIKFACWALSVMKKGGFAIIPIKSKKVDRHGLLPDR
                     HISLWQYTDISDKRLILNENFIFVQQDVEIERPFKIGTFVNSNWIAYYVDGLLFVKKI
                     ALYEGEYPDFGCNVEVYTNSQFLELETLSPLKTVEPEGYIEHKEIWDLIEIENLKPDE
                     KEIKRILKDYLPLG"
     gene            complement(8818..9696)
                     /gene="xerC"
                     /locus_tag="PLF90_02615"
     CDS             complement(8818..9696)
                     /gene="xerC"
                     /locus_tag="PLF90_02615"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOK56210.1"
                     /translation="MKEELNKFIDYLENQKNYSKHTVKGYKKDISQFIEFLKEKKISD
                     FEKVEYEDFISFIGKLKNSKLKEKTIGRKVASLKSFYKFLVMRKYIRKNPAILIQSPK
                     IPEKLPNFLTYNEISRILEIPFDKKNWQTLRDKAILEILYSTGIRVGELVNLKLEDIN
                     FVDEVIKVKGKGKKERIVPIGKPALNSLIEYIEKRPNKKEKFLFLNKYGKKLTERTIE
                     RLVKKYSLISGINKNVTPHTIRHTFATHLLDRGADLRTVQELLGHERITTTQIYTHLT
                     IEKLKELYEKFHPRGK"
     gene            complement(9677..10003)
                     /locus_tag="PLF90_02620"
     CDS             complement(9677..10003)
                     /locus_tag="PLF90_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56211.1"
                     /translation="MEKNNLPNPRISAILSFIFNGLGQIYNGEIKKGLTLIFLSGVSL
                     LILILGAIFIFYFIKTLPPISFLIWGVCLFFFGLTSIIIIGIYSISDAYNKAKKILEE
                     YEGRTK"
     gene            complement(10021..10530)
                     /locus_tag="PLF90_02625"
     CDS             complement(10021..10530)
                     /locus_tag="PLF90_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HOK56212.1"
                     /translation="MNKKTIKFFIVIFIHILILTGVALYYKWTKKGITVFLKIGRYDP
                     MSPLRGDYLTLFYPEISRIPFTEFASSVSIGDTFYVPLEKKDKIWIRKKDVKIDKRKE
                     YEGIFIKGMVKNVGSYVILEYGIETYYIPVGTGEDIKWSDNDYAEIFIDENGNGKIKK
                     IYLNGKQFP"
     gene            complement(10651..11604)
                     /locus_tag="PLF90_02630"
     CDS             complement(10651..11604)
                     /locus_tag="PLF90_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56213.1"
                     /translation="MVFGYLLLILIVFLLFQGARNFLSFLFKIKQHPEKEIFNLTINR
                     ENTVGQIFIILSIIFFGESLLILNRYFSGNLSFYWVIFIVILISLILGYFLESLYSII
                     FGIIFLPCWWISHSVVWIEKRTIATNIILSGLYFIILIYYLSGRILEKNKKWEKTGII
                     MHFLSIPMLFTFLFLLSTPYGISLIEGNLGSPNILLSKPLTFFILLFFFSSTILLFYA
                     SFKNFISISLEFIPLFFILCFFLSFLFFQIPQLTNMEYTYKRSFVESFTPQGIFFVIF
                     LNIITFSFSLLLFFPWISKKRRDTCKFWCSVYVYFCNISVF"
     gene            complement(11607..12263)
                     /locus_tag="PLF90_02635"
     CDS             complement(11607..12263)
                     /locus_tag="PLF90_02635"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HOK56214.1"
                     /translation="MINFEELKKEGERWVSEGLITEQQLEKILQIYIPEQKPSEDELR
                     KNVTRLILIFASVFIGIAFFSFVASNWKYLSFLTKFFIIVICMLSSYISGWYFIEFKN
                     LKRTGEALIYLGQLIFGSGIFLIGQHHHTPAYNWPLGFILWTIGTILLAFSTNIFSLF
                     YFAIVLSIIPVIGFPFIIFDERIISQFFFTPLWIVLFMSFFLFISGFIIYKKGKKQGI
                     "
     gene            complement(12280..13059)
                     /locus_tag="PLF90_02640"
     CDS             complement(12280..13059)
                     /locus_tag="PLF90_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008731394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOK56215.1"
                     /translation="MKRHKKEVSLFESFIKEEDLIEEKESTEEDEKPPFETTTLWDYP
                     KQSYGKKTKGDNKFQGVTPAFIIWNLIQRYTKLGDLVVDPMAGSGTTIDVCEEEGRRV
                     IGYDINPKHPKVIKNDSRHIPLPDNSVDMVFIDSPYGDNVNYSDDPKFYEELEKVAIE
                     IKRILKPGKVLGWLIGNQWVKGKFTPVGFKVYQMLEKHFEPIDIICVARRGQSSNTPL
                     WHYRARKFNFFLRGFKYLLLFRKEEKKEEERNRKIKWKKYK"
     gene            complement(13046..13486)
                     /locus_tag="PLF90_02645"
     CDS             complement(13046..13486)
                     /locus_tag="PLF90_02645"
                     /inference="COORDINATES: protein motif:HMM:NF036920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaWI family type II restriction enzyme"
                     /protein_id="HOK56216.1"
                     /translation="MERKKAENLTEELSKVKRNLLVDYGEYGSHLPDVDLIIYEPESC
                     KIIAVISSKVTLRERIAQTGYWKIKLSQDKITKDIKVFFITPDEDRTLSNKKPCKKGR
                     AIVEIDTNGGYILNEESIEESEKVKKFDKFVHDLKKLLGKNEKT"
     gene            complement(13746..14330)
                     /gene="hisB"
                     /locus_tag="PLF90_02650"
     CDS             complement(13746..14330)
                     /gene="hisB"
                     /locus_tag="PLF90_02650"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOK56217.1"
                     /translation="MPRKKKFKRKTKETDIEIEINLDGKGDSEIETPIGFLNHMLELF
                     SKFSNFDLKIKAKGDIEVDNHHLVEDTGICLGSAIKEALGDKKGIKRFGFSSTPMDEV
                     LVNISLDISGRPYLVFNVPIIKGREGSFEIEDSKEFLKGFVNHLGLTLHINLVYGDNL
                     HHILEGIFKGIGLALKEAVKIEGKKLPSTKGKID"
     gene            complement(14351..15013)
                     /locus_tag="PLF90_02655"
     CDS             complement(14351..15013)
                     /locus_tag="PLF90_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOK56218.1"
                     /translation="MKNKKIGFTLIELLVVIAIIAILAGMLLPALSKAREKARQATCM
                     NNLKQLYVALEMYANDNEEFYPFCQGRCFWGTGNISPPPNLGYPGWTEQLYSYVKNKN
                     IYRCPTYPVKNDQFTYFLSARAAYIDAGNQRAATNRKKIRYPSAFVLAGDNEYKNFEV
                     NDNDKDDYSQNCLSWEETEDHWAPYHLGGLNVLFADGHVSWIREYDPQKITFRYYDFS
                     NW"
     gene            complement(15081..16004)
                     /gene="rbsK"
                     /locus_tag="PLF90_02660"
     CDS             complement(15081..16004)
                     /gene="rbsK"
                     /locus_tag="PLF90_02660"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOK56219.1"
                     /translation="MKRTPVITVIGSSNIDLVVKTSHYPSPGETVFGKQFFMCPGGKG
                     ANQAVVASRLGAKVNFISCIGNDFFGDVIIKNLKKENIKTESIIRDPGSPSGIALIII
                     DKKGENSIVVNPGANYKLGKKDIEKHKKLIFSSDILLLQLEIPIDTVEYAVKLAKKKN
                     IPVILNPAPAKKIPDSLLKNIDILTPNKIELEKLTGENLNSINSIKKASYKLLHKGIK
                     TVIVTLGERGVFLVTKTLSEIVPSKKVKAVDTTGAGDAFNGALAFAIAEGKDLTQAVY
                     FANIVAALTVTKIGAQSSPGKEEVEKFLKNP"
     gene            complement(16001..16678)
                     /gene="rpe"
                     /locus_tag="PLF90_02665"
     CDS             complement(16001..16678)
                     /gene="rpe"
                     /locus_tag="PLF90_02665"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOK56220.1"
                     /translation="MKKKIKISPSLMCADLSQLGEEVKKLEEAGADSFHFDIMDNKFV
                     PNITFGPDIVESLREKTFLPFEIHMMVYQPENLIEKFIKAGGNIIIVHYEAVLYPYRI
                     LNELKNKNIKTGIAINPKTPVCCIEELIKEIDTVLVMCVEPGFAGGNFIPSSIKKIKE
                     IKNIAEKVNSDLSIEVDGGVNEKTIPLLIKEGADIFVLGSTSIFREERDYKKRIKDIR
                     KLIEKYL"
     gene            complement(16762..17952)
                     /gene="hisD"
                     /locus_tag="PLF90_02670"
     CDS             complement(16762..17952)
                     /gene="hisD"
                     /locus_tag="PLF90_02670"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOK56221.1"
                     /translation="MEKISFEELKKILERKEEDIEKGVKEIIKRVKEEGDKAIFYYTE
                     KFDRVKLENLKVNEDEIKESRKYLKGEIFEYIKKAKERIEKYHKKQLPKGFKIKEKYI
                     EIEFRYQPIEKVGIYIPGGQSPLISTILMTVIPAKVATVKEIYVCSPPSYNGKIHPLI
                     IATCDYLGIKNIFSAGGVCAISAFAFGTETIPKVDLIAGPGNKYVNCAKKLLFGKVGI
                     DLPAGPSEVVIFSDSSGIPDFIEYDLKAQIEHTDGTGILITTDEKIGKNIEKKVEKGY
                     WILVKNEKEAIEIINYIAPEHLEVISKKPFLFKNCKAGAIFIGNYTPVTIGDYFAGPS
                     HVLPTGMSANFSSGLSVFTFLRSFAVIKCKKEFIKEYGKYIEKIAEVEKLESHKNSIK
                     IRKS"
     gene            complement(17940..18590)
                     /gene="rpe"
                     /locus_tag="PLF90_02675"
     CDS             complement(17940..18590)
                     /gene="rpe"
                     /locus_tag="PLF90_02675"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017796627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOK56222.1"
                     /translation="MVKIAPSLLSADFSRLGEEVKKVTEAGADLIHFDVMDGHFVPNL
                     TFGPMVLAAIRRYSHLPFEAHLMITNPEKYWKNFADSGADIIGIHIECEVDHKYIITE
                     IQKYGKRACIVINPPTPVEKIYPVLEMVDMVLIMTVNPGFGGQKFIYEVVEKIKKVNE
                     YRKNKKLNFEIEVDGGINKETGKIVVENGVDILVAGNYIFSGNDYKERIESLRWKR"
     gene            complement(18612..19283)
                     /locus_tag="PLF90_02680"
     CDS             complement(18612..19283)
                     /locus_tag="PLF90_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK56223.1"
                     /translation="MDNNFKNPLIELKNITVSYPERGKVLDNLSLSISEKDRIGIKGA
                     NGSGKTSLLYTIVGLVKIEKGEIKIFGKKMEKESDFVEVRKKIGFLFQDSDDQLFSPT
                     VEEDIAFGPLNLRLPKEEVKERVERTIKLLGIENLKHRFSHTLSYGEKRIVAIATILS
                     MEPEIYLLDEPTTGLDEKTSQIIEKFLIQNNLTYLMVSHDINFLERVCNKIYLLENGS
                     LLSIK"
     gene            complement(19256..19966)
                     /gene="cbiQ"
                     /locus_tag="PLF90_02685"
     CDS             complement(19256..19966)
                     /gene="cbiQ"
                     /locus_tag="PLF90_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOK56224.1"
                     /translation="MEKIDPRLRLLTSFVYSIFVALERNFEIFKFYLILPVILFFFIT
                     DFKKFFKGLISVNLFIFLCWLFLPLSIPGKEVFRFLKFSVTYEGIRYTLLITIKANLI
                     FLTNFVLIFSTHPIRIIHALHHLHLPKKLINIFFLSTRYIPVIEKEKNRIQKAMKIRC
                     FVPKNNIHTYKTIGNFVGILLLRSYERSERIYKAMILRNFSGIFWTYHHFVWSKKDTF
                     VSFCVIIYFLWIIILKIH"
     gene            complement(19954..20511)
                     /locus_tag="PLF90_02690"
     CDS             complement(19954..20511)
                     /locus_tag="PLF90_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56225.1"
                     /translation="MKKLIFIFFIFSVITFAHKINIFTYKEGNKIFVEGYFQDGSPCK
                     NSPVEVYNEKSEKILDGKTDEKGIFSFDIPDAEKIKIVLTADMGHKVETEMELKKKTE
                     IKKEEKKVEKKEEKTEIQKGIDEEKIKEIVEESVEKAINPLIKEIEKEKQKIKLTDII
                     GGIGYIFGILGIYLYFRGRSSEWKK"
     gene            complement(20512..21099)
                     /gene="cbiM"
                     /locus_tag="PLF90_02695"
     CDS             complement(20512..21099)
                     /gene="cbiM"
                     /locus_tag="PLF90_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HOK56226.1"
                     /translation="MHISEGVLSGPILGIGAVITLAGTYKGLKDLEYKDYPKVSLLTS
                     AFFVASLVHVPVGPSSSHLVLNGLCGIILGWKSFPCILLALFLQALLFQFGGLTTLGI
                     NTANMAIPAVISYYIFKPFLKKNYFLTGFLSGFFSIILSCVFVFFSLYFTGSFFENVG
                     KTIFVVHLPIAVIEGFITGFILSFLSKTKKELLEV"
     gene            complement(21101..21865)
                     /locus_tag="PLF90_02700"
     CDS             complement(21101..21865)
                     /locus_tag="PLF90_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HOK56227.1"
                     /translation="MNKKLVLYLFLSIFLNSFAHFGMIIPSPDIVEEKEKANINLKIL
                     FAHPFEGKTMDMEKPSDFGVFFRGEKISLLNEIKETSYKFYSDEKIHRGWSLSYTLKR
                     PGDYIFYCIPSPYWEPAEEKYIIHYTKVIVNGFGMEDSWDKEIGLKVEIVPLVRPYGI
                     YAGNTFYGIVKFNGKPVPFCDVEIEYFNEKGNIKAPYSPFITQVVKTDQNGVFYYSFP
                     KSGWYGFSAICTDEKKINDKEVEVAGVLWIKVYEMK"
BASE COUNT         7747 a         3135 c         3128 g         7914 t
ORIGIN      
        1 gcttttgcca atttcctcac ctccttttaa ttttttttaa aattttacat tcctttttat
       61 atttgtcaaa ttttacatga tttttctttt tctgttaatt ttttaaaatt tgataattgt
      121 agaaatatca atgtttttga aaattacttg acagaataga aattttaata tagaattatg
      181 actgactggt cagtcatatg gaaaataaaa gaaaattaat tataaaagtc gcggaaaaat
      241 tgataagtca aaagggatat tcaaaaacca caatagaaga gataacaagg aaggcaggaa
      301 taggtaaggg aacattttat ctttatttta aaaataaaaa tgatttattt ttttcaataa
      361 ttaaagaaga gtttgaaaat cttatgctaa aaattaaaaa agaggtggaa ggtattgatg
      421 atttttttga aagattaaaa aaagagattg aaatgtatct tgactatcac gaaaaaaatt
      481 atgctttttt taaaatatta cttcaggaaa aaccatttat aagaaaaaag agttttgaaa
      541 aattctggaa ggattttttt gaaagatggg ataattttat gaagaaggga tttgaagaac
      601 agataaaaaa gaataaaata aaaaaatttg atttagaaga tataatttac tcattgattg
      661 ggatattgca tggaaatatt cacagatgga tactaaatga aagaaaatac tcacttgtaa
      721 aaaagaaaga ggttattttt aaaatattta tagaaggaat caggagataa aaatgaaaaa
      781 atttttaatt ttctttttcg tttttgtttt aatttctttt tctgaagaag tgtttaatct
      841 tgatgaatgt ataaaaattg ctattgaaaa caatttaaag ttaaaaattt cacaggataa
      901 aatagatagt gcatattatc aaaaagaaat agccggaaaa tataaactcc caacattttc
      961 aacatccttt aattatacac accttggaga taatgaaggt tttgttttta atggaattcc
     1021 tatgaagttt acagaagaca atattttttc acttaaattt acattaagtc aacctatttt
     1081 tactggctgg aaaattgaaa aaacatatga aatatcaaaa gaaaattatg aaaaaacaaa
     1141 aattgatttt gaaaatgaat tatccaattt aatatttgat gttgaaaaag catattataa
     1201 tattctgaag attaaaaaaa atcttgaaac agggttaaaa tataaagaaa atcttgaaaa
     1261 acatctttct gatgcaaaaa aattatttca ggaaggaatt gtaacgaaac ttgatatttt
     1321 aaagacagaa gtcgctttaa aaaatgcaga aacaaggata attgagatag aaaattattt
     1381 aaaaactgca aaagcaaatt taaattttct tctgggaaaa tctcctgacc atgaatttga
     1441 aatagaagat attcttgaag taaaggagga aaaaaaagat tatcagtggt ggaaagaaat
     1501 gtctttgaaa gaaagaagtg aaataaagag tatggaaaag ttaatttcaa tttatgaaaa
     1561 aaatatagac attgagaaaa gtaatttata tccacagatt tattttttct ttaattacaa
     1621 tattgaaagg ggttctcaaa catctgttgg aaaatgggat actaactgga atacaggttt
     1681 tttgctatct tatgatattt ggaactgggg tcagacaaag gataagataa gaaaagcaga
     1741 gattgagaaa gaggaaatta aaaagcagtt tgaattaatt aaaaattcaa tagaaataga
     1801 ggttaaaaat gcttatttaa actttctatt agcagaaaat aaaattgaag aaagtaaaaa
     1861 acagatagaa cttgcagaag aaaattttag ggtggcaaat cttctttata atgagggact
     1921 tgcaactaat actgatgtga ttgacgcgat tactgcatta acagatgcaa aaaataatta
     1981 ctataattca ctgtatgagt ataagatatc ttacagtcaa cttcttaaag caagtggtat
     2041 tttaaagtag gagggaaaat gaaaaagatt atatgggttt taatagtttt gattataatt
     2101 cttgtagttt ttaatcagtt taataaaaag aaaaaagaaa aaaaagtgga aattgtgagt
     2161 gaaacagaaa taccggttga agttgaaaag gtcagtgaaa ttgttttaaa agatgaggtt
     2221 gaatgtatag gagagattga accttatcag aaagttgttg tatattctga aatttcagga
     2281 cagattgaga aattgagtgt aaaagaagga gattttgtta aaaagggtga ttttatagta
     2341 cagattgatt ataaaaaaag aaaaattgat tatgagaata tggaaaatca gataaaagca
     2401 atggaggcaa atcttgaaaa tataaaaaag gactatgaaa gatttacaaa attatataaa
     2461 gagggtgtaa tttcagaaaa aaaacttgat gatataaaaa caaattatga agttgtttcc
     2521 catcaactgg aatcattaaa aacacagttt gaacttgtaa aaattcgtct taatgaagca
     2581 tttatttatt ctccaataac tggatttatt gctgaaaaat ttatagatga aggagagtta
     2641 attacagaaa gttctatgat gaggtctgtt cctattgttg ccattgtaga tataagcaaa
     2701 gtaaaagtta aattgcctgt ttcttctgaa gaaattggaa aggtaaaaac agggcagagt
     2761 gttttaatta aacttgatgc atatccaaca aaggattttt atggtaaggt ttccaaaata
     2821 tatcctgttg cagatgaaaa aacaaggaca gttgatgttg aagttattgt tgaaaataaa
     2881 aactatctta taaaacctgg gatgtactgt aagggaatta ttataacagg agagaggaaa
     2941 gttcttgcta ttcctcttga tgcggttatg aaattgcctg caagtggtaa ttattactgt
     3001 tttaaggtta aggataatac tgctgaaaaa gtgtatataa aagttgggaa aataaaagaa
     3061 aatcttatag aagttaaaga agggctgaac agagatgata ttattattac aacttctcag
     3121 ggaattcttg atacagggaa aaagataaaa ataataggag agaaaaaatg aacttaccag
     3181 agttttcagt taaaaggcct tatacaactt taatgatttt ccttgcgatt ttaattgttt
     3241 cttttgtaac aatacctaaa atacctgttg accttatgcc tgaaattgaa gttcctgcgg
     3301 tctctgttat tgttccttat ccgggcgcaa gttcttcaga tgttgaaaat gatgttgtta
     3361 aatatcttga ggattatctt acaggagttg aagaccttga tgaattaatt tccgtttcaa
     3421 aagataacgt aggaattgta acatgtaaat ttaaatgggg taaaaatctt gacacagcag
     3481 ttaatgatgt tagggataaa gttgattttg caaagaaaga tatttatcag catgcacctg
     3541 atgcagaaga accgatgata tttaaatttt caacatcaat gacacctgtt ttaatttttt
     3601 ctatatcttc aaaaacactc tggggcgatc tatattatat tgctgataaa cagatagcag
     3661 accagttaaa aagagcagaa ggggtaggaa cagttatggt ttttggtgga ataagaagac
     3721 agattaatat ttatcttgac tgggaaaaaa taaatggtta tagaatacct ccaaatttaa
     3781 tagttcagcg acttgcagaa gaaaatattg attttcctct tggtgagata aaaaggggta
     3841 aaagaaatta tttcttgaga actgaaggaa gatatgttaa tccagaagag attaaggaca
     3901 ttttaattac aactattgaa ggaagacctg tatatttaag agatattgca aaagtggatg
     3961 attattacga ggaggtaaaa tcaaaagcat atacagatgg agaggaaaca gttatcttga
     4021 tggttcagaa acagtcagga aaaaatactg ttattgtttc taataatgta aaaaggaaaa
     4081 tagaggagat aaaaaagttt cttccaaagg atattaaaat taatactgtt tttgacacct
     4141 ctgaaatgat attaaagtca attaaaaacc ttataaatac tgttataact gcaggaattc
     4201 ttgttattct tgttactgtt tttcttttaa gacggctgaa attaagtttt attgtttcaa
     4261 tttcaatccc tttttccttg atatgtgcat ttttgtttct ttatatcggt ggatttacaa
     4321 taaaccttat atcccttatg tctcttgcac ttgtaatagg tatggttgtt gataattcaa
     4381 ttgttattct tgaaaatatt gtaagacatt atgaagaggg agaaaaaata gatgaagcaa
     4441 gtatttttgg agcaagtgaa gttggaactg cgattatcgc ttcaactatt acgacaatag
     4501 ttgttttcgt tcctttaatt tttacaaagg gttttatagg tataatattt aaacaacttg
     4561 catatattat atctacaaca atctttatgt cccttgtagt tgctctttta cttgttccta
     4621 tgctttcttc aaaaattatt aaaggtcatc ctaaggagac aaaattttat aaaataggag
     4681 agaaattctt aacaaaaata gatgatgtat acacaaattt acttacatgg tgtctgaaac
     4741 ataaaaatct atttctttct ttaattataa ttgtgttttt tgcttctctt cttcttttta
     4801 aatttattgg agttgaattt tttcctgaag cagataatgc aagaatagaa acaacaattt
     4861 taatgaatcc gtcaacaagg attgaagaaa cagataagat tgtaagagaa atagggaaga
     4921 tatatgaaga ggaaatacct gaaagaaaag catggtatgg aagttgcgga gaatctggtt
     4981 ctggtatagg agcagcaatg ggtagagatg aaggacctta tgcagggagt atttctgcac
     5041 gacttacatg gagagaggaa agaaaaaggt ctgataagga aatagcagaa attataagaa
     5101 aaagaataaa agaaatacct ggaatagaaa aattttctgt ttcaattgca agtccattac
     5161 agttaatgtt tatgggtgga gcaaaacaga tagaggtaga acttaaaggt tcagacctaa
     5221 ataaaattgt aaaatattct gaaatgttga aagaaagaat tgaaaaaata ccaggagcag
     5281 tcaatgttga tacatcttac aaaaaagaca gaattgagat acatttaaag attgacaaag
     5341 aaaaagcaaa aaattttggt atttcaacat cttccatttc tcaaatagta agaagttata
     5401 tatatggata cactgcgaca aaatatagag aaggtgggga ggattttgat gtatttgttc
     5461 aacttgaaga aaagcagaga tatgatattg aaaaaataga agaattgcct ttgtttactc
     5521 catctggaag agttattaag ttaaaggatg ttgctgaaat agattttgaa ctcggacctg
     5581 tccagataga tagaaaaaac agacagagaa ttgtaaaggt aagatgtgat acattcaaaa
     5641 gacctatgag tgaggtattg aaagatattg agaaagaagt agaaaaatta aatataccag
     5701 aagatatcac agttagttat gggggtatgg ttaaacagca gagggaaagt tttgctgatt
     5761 taagattatt acttatcctt ggagttattc ttgtttatat ggttatggtg ggtcagtttg
     5821 aatcatttaa aacacccttt ataattttct tctcaatccc atttacattt atcggaagca
     5881 tattattttt actcataacc ggagtaaatt tctctattgc ttcatttatg gctataataa
     5941 tgcttatggg agttgttgtt aataacgcaa ttgttcttgt tgattatata aatattttaa
     6001 gagcaagggg atatcagttg tttgatgcaa taacaaaagg agcaagaaat agaataagac
     6061 caattatgat gacttcttta acaacaattt ttggaatgat acctatggca cttggaagag
     6121 ggactggtgg agagatatgg caatcctttg gagttcctgc aataggtggt ttccttgttt
     6181 catggattat aacactttta cttgttccta taatttattt aattatgaat aaggagtcaa
     6241 aatgaagatg tttttaattg tttcaaatat aggaattcac gaagaaatcc ttgatattct
     6301 gaaagagtgt aaagttgaag aatatacaag gatagaaaag gttcagggag ttgggaaaac
     6361 agcaggtcca catcttggaa caaatgtgtg gccatcctgg aatactttat tttttgcaca
     6421 gttgttgagg atgaaaagga aagtttatta ttagaaaaat ttaaagaagc aaaagagaag
     6481 tataaaaaga aaggtttaaa agtttttatt cttccggttg aagatgttat ttaaaaaaag
     6541 gggataagat gaaaaaaata ttattcttga tttttttaat tttttcttca ggtttctgtg
     6601 aaattagaaa ttttaaggtt gatttttata ctggttatgg ctttgcaaaa attcaaaatc
     6661 caaaaaatta tgaattttat acattgatgg ttgacttcac aagaccgatt aaaaacagta
     6721 aaaatttttt ttatcagata gaaccatatg tttcttatgt cttcagtcca gccgataatt
     6781 ttgaaatagg agtttctggt tttttattgt ataaattctc tgatagtaat ttccagccat
     6841 atttgaaagt tggtacagga ataatttatc tttcatcaga ttttgttgaa caatccactc
     6901 attttaattt tgcatcatca gtaagttttg gagtaagggt ttttttgaag aaagtttttt
     6961 taaatactga gataagatta aggcatgttt caaatgccgg aataaaattg cccaatgaag
     7021 gaattaattc taaacttttt ctcatcggta taggcagtga tttttaaaat ggaaaaagtt
     7081 tttatagatg ttcactgtca cgtttttaaa agaccttaca tatggattga tggaaaatcc
     7141 ctcttttctt ctcctgaaca agttataaaa agatatgatg aacttggtat agaaaaagga
     7201 gttttattac cgattgtaaa tccagaagtt tatcttcctc aatcaaatga agaaataata
     7261 gaaatctgtg aaaaatatcc aggaagattt ataccattct gtaatattga ccctcgtgct
     7321 ataacaaatt caccagaggc agattttgaa aaaattttaa attattataa atctctcggt
     7381 tgtaaaggaa ttggagaagt tatgccaaac cttccatttc ttgacgaaag agttttaaat
     7441 ttatttagac atgctgaaaa agttggtttt ccgattattt ttgatggaaa tggtaaactt
     7501 ggaaatactt atggattata tgatgaaccg ggattacctc aacttgaaaa atgtctttct
     7561 ctttttcctg atttaaaatt tatcggacac ggtccttctt tctggtcaga gatatcggtt
     7621 ctaaaagatg aggaagatag atatggatat cctgattatc ctgttattga agagggaatt
     7681 gtccctgtcc ttttcagaaa atacagaaat ctttatggag acctttcagc aagaagtgga
     7741 tataatgcac ttaaaagaga cactgaatat gcggttaaat ttttaagtga attttctgac
     7801 cgtcttttat ttggaactga tttatgtaga cctgaccagg atactccact tatcaaattt
     7861 ttaaaaggtc ttgttgaaaa gggaaaaata agtgaaaata tattcagaaa aattgtaaga
     7921 gaaaatgcta ttaaacttct aaatctataa ataggagaaa aaaatgaaaa taaaaaagtt
     7981 tgaaatgggg gaatttatta ttggtatacc cgaagatatt ggaccaagga ttattcattt
     8041 ttcgcttaaa aaatatcctg atagaaatgt ttttgaagtt attccagacc ttacaataga
     8101 tacagaagaa gggaagtggc agatttatgg aggacacaga ttatggactt ctccagaaga
     8161 taaacctcgt tcctattctc ttgataataa acctgtaaaa attgaagaaa agagtaagaa
     8221 tataagtatt tctggaaatg cagaaataca gaattctgta aaaaaagaaa ttgaaataaa
     8281 aaaaacaaag gaagggattg aagtaaacca caggataagg aatattggaa gatggccaat
     8341 taaatttgca tgctgggctt tatctgttat gaaaaaaggt ggttttgcaa taattcctat
     8401 aaagtcaaaa aaggttgata gacatggact tttacctgac aggcatattt ctctatggca
     8461 gtatacagat atttcggaca aaagattgat tttaaatgaa aattttattt ttgttcaaca
     8521 ggatgtagag attgaaagac catttaaaat aggaacattt gtaaattcaa actggattgc
     8581 ttattatgta gatggtcttt tatttgttaa aaaaattgct ctctatgaag gagaataccc
     8641 tgattttgga tgcaatgttg aggtctatac aaattctcaa tttcttgaac ttgaaacatt
     8701 aagtccattg aaaactgttg aaccagaagg atatattgaa cacaaagaaa tatgggattt
     8761 aattgaaata gagaatctta agcccgatga aaaagagata aagaggattt taaaagatta
     8821 tttacctctt ggatgaaatt tttcatataa ttcttttaat ttttctatag ttagatgtgt
     8881 ataaatctgg gttgttgtta tcctttcatg tccgagtaat tcctgaacag ttcttaaatc
     8941 tgctcctcta tcaagaagat gtgttgcaaa agtatgtctg attgtatgag gggttacatt
     9001 tttatttatt ccagatatta atgaatattt ttttacaagt cgttcaatag ttctttctgt
     9061 taatttttta ccatatttat ttaaaaataa gaatttttcc tttttattcg gtctcttttc
     9121 aatatattca atcaaactat ttaaagcagg tttccctatt ggaactattc tttctttttt
     9181 ccctttaccc tttactttta taacttcatc cacaaaattg atatcttcta attttaaatt
     9241 tacaagttct ccaaccctta ttccagtgga atataaaatc tctaaaattg ctttatctct
     9301 taatgtctgc cagttttttt tatcaaaagg tatttcaaga attctggaaa tttcattata
     9361 ggttaaaaaa tttggcaatt tttcgggtat ttttggtgat tgaattaaga tagcaggatt
     9421 tttccttata tattttctca taacaagaaa tttataaaat gattttaatg aagcaacctt
     9481 tcttcctatt gttttttctt ttaattttga attttttaat ttacctataa aacttataaa
     9541 atcctcatat tcaacttttt caaaatcaga gatttttttt tcttttaaaa actcaataaa
     9601 ctgtgatata tcttttttat aaccttttac tgtatgtttt gaataatttt tctggttttc
     9661 aagataatca ataaatttat ttagttcttc cttcatattc ctctaaaatt ttttttgcct
     9721 tattataagc atctgaaata ctgtaaattc ctattataat aattgaggtt agaccgaaga
     9781 aaaaaagaca aactccccat attaaaaaac taattggagg aagtgtttta atgaaataaa
     9841 agataaaaat tgctccaagt attaaaatta aaagagagac accggaaagg aaaataagtg
     9901 taagtccttt ttttatttct ccattataaa tctgcccaag tccattgaaa ataaaagaaa
     9961 gtattgctga tattcttgga ttcggtaaat tattcttttc catttttgta gattctatta
    10021 ttacggaaat tgtttaccat ttaaataaat cttttttatt ttaccatttc cattttcatc
    10081 aataaatatt tcagcataat cattatcact ccatttaata tcctctcctg ttccaacagg
    10141 tatatagtag gtttcaattc catattctaa tataacataa cttcccacat ttttaaccat
    10201 cccttttata aatattccct catattcctt ccttttgtct atttttacat ccttttttct
    10261 aatccatatt ttatcttttt tttcaagtgg aacataaaaa gtgtctccta tagaaacaga
    10321 agatgcaaat tcagtaaatg gtatacgtga aatttcagga taaaatagtg ttaaataatc
    10381 acctctcaat gggctcatcg gatcatatcg tccaattttt aaaaaaacag tgataccttt
    10441 ttttgtccat ttataatata gagcaacacc agttaaaata agaatatgga taaaaatcac
    10501 aataaaaaac tttattgttt tcttattcat tgtactcctt taattttttc aatatatttt
    10561 cttcttccct tttccataaa gtaacccaat acaaaaagca gtatcccacc tccaaggaaa
    10621 aacaaacttt tatttaaaaa tttgaaataa ttaaaatact gaaatattgc aaaaatagac
    10681 ataaacagaa caccaaaatt tacaagtatc tcttcttttc tttgatatcc aaggaaaaaa
    10741 aagtaaaaga gaaaatgaaa aagtgattat atttaaaaat ataacaaaaa aaataccttg
    10801 tggagtaaaa ctttcaacaa atgacctttt gtaggtatat tccatatttg taagttgagg
    10861 aatttgaaag aataaaaaag acaaaaaaaa gcacaggata aaaaataaag gtataaattc
    10921 aagggaaata gaaataaaat ttttaaatga agcatagaaa agtaagattg ttgaagaaaa
    10981 aaagaataaa agaataaaaa atgttaaagg ttttgaaaga agaatgtttg ggcttccaag
    11041 attcccttca ataagtgata ttccataagg agttgaaaga agaaaaagaa atgtaaaaag
    11101 cattggaatt gataagaaat gcattattat tcctgttttt tcccattttt tatttttttc
    11161 aagaattctt cctgaaagat aataaattaa aattataaaa taaagtccac tcagtatgat
    11221 atttgtggct attgttcgct tttcaatcca tactactgaa tgggaaatcc accagcaggg
    11281 tagaaatata ataccaaaaa taatagaata aagagattca aggaaataac caagaattaa
    11341 agaaatcaat attacaataa aaatgaccca gtaaaaagaa agattacctg aaaaatatct
    11401 gttaagaatt agtagtgatt caccaaaaaa aataatgctt aaaattataa atatctgtcc
    11461 gacagtattt tctctattta tggtgagatt aaatatttct ttttcaggat gttgtttaat
    11521 tttaaagagg aaagagagaa aatttcttgc tccttgaaat aaaagaaaaa ctattaaaat
    11581 caaaagaaga tatccaaaca ccattttcaa atcccctgtt tttttccttt tttataaatt
    11641 ataaatcctg aaatgaaaag aaaaaaactc ataaaaagaa ctatccataa aggtgtaaag
    11701 aaaaactgac ttattattct ctcatcaaaa ataataaaag gaaatccaat aacaggaatt
    11761 attgataaaa ctattgcaaa ataaaaaaga gaaaaaatat ttgttgaaaa agcaagtaaa
    11821 attgttccta ttgtccataa aataaaacct aaaggccagt tataagcagg agtatgatga
    11881 tgttgtccga ttaaaaaaat acccgaacca aagattaact gtccgaggta tataagtgct
    11941 tctccagttc tttttaagtt tttgaattct ataaaatacc agccagaaat ataagaagaa
    12001 agcatacata taacaattat aaaaaatttt gtaaggaaag ataaatattt ccagttagaa
    12061 gcaacaaatg agaaaaaagc aattccaata aaaacagatg caaaaatcaa aatcaatctt
    12121 gtaacatttt ttcttaattc atcttcagag ggtttttgtt caggaatata tatctgtagt
    12181 attttttcaa gttgctgttc tgtaattaac ccctcactta cccacctttc cccttctttc
    12241 ttcaattctt caaaatttat catttttgta aagataattt tacttatatt tcttccactt
    12301 tatctttcta tttctttctt cttccttttt ttcttctttt ctaaataaca gtaaatactt
    12361 aaaccctctt aaaaagaaat taaattttct tgctctgtaa tgccacaaag gtgtatttga
    12421 actttgtccc cttcttgcca cgcatattat atctataggt tcaaaatgtt tttcaagcat
    12481 ttgataaact ttaaatccaa caggagtaaa tttcccttta acccattgat ttcctattaa
    12541 ccatcctaat acttttcctg gcttaagaat tcttttaatc tcgatagcga ctttttcaag
    12601 ttcttcataa aattttgggt catcggaata atttacatta tctccataag gagaatctat
    12661 aaaaaccata tcaactgaat tatcaggtaa aggaatatgt ctggaatcat tttttattac
    12721 tttgggatgc tttgggttta tatcataccc aataactctc cttccttctt cttcacaaac
    12781 atcaattgtg gttccacttc ctgccattgg atcaactact aaatctccta gttttgtata
    12841 tctttgtatt aaattccaga ttataaaagc aggagttaca ccttgaaatt tattatctcc
    12901 ttttgttttt tttccataac tttgtttagg gtaatcccag agtgttgttg tttcaaaagg
    12961 tggtttttca tcttcttctg tactttcttt ttcttctatt aaatcttctt cttttataaa
    13021 agattcaaat aaactaactt cttttttatg tcttttcatt ttttcctaat agttttttta
    13081 aatcatgaac aaatttgtca aattttttta ctttttcact ttcctctata ctttcttcat
    13141 ttaaaatata ccctccattt gtgtcaattt caacaattgc cctgcctttt ttacatggct
    13201 ttttattact caatgttcta tcttcatcgg gtgtaataaa aaatactttt atatcttttg
    13261 ttattttatc ctgagaaagt ttgattttcc aataacctgt ttgtgcaatc ctttctctta
    13321 aagtaacttt actggaaata acagcaataa ttttacaact ttcaggttca tatattatca
    13381 aatcaacatc gggtaaatgt gaaccatatt ctccataatc caccaacaaa tttcttttaa
    13441 cttttgaaag ttcctctgtc aaattttctg ctttttttct ttccaaggta tttccttcta
    13501 ttatttttaa tcctaattcc tcaacagcat cttttatgat ataatgtatc agtttttcca
    13561 gatttttccc tttaaatgat ctccatgatt gttcatggtc tttgtttgga gttggatttt
    13621 ttaaccagtc ctttttatga atttctttgg cttcttttag taaatcagaa attttattat
    13681 atgcattttc ccaaatttat ttttatactc ttcataaagt ttttttaggt tattgaaatt
    13741 catttttaat caatcttccc tttagtggat ggtaattttt tcccttctat ttttaccgct
    13801 tctttaagtg ccagcccaat ccccttgaaa ataccctcaa gaatatgatg taagttatct
    13861 ccataaacaa gatttatatg aagtgtaagt ccaagatgat taacaaaacc cttcaaaaat
    13921 tcttttgaat cttcaatttc aaaactccct tctcttcctt ttataattgg aacattaaaa
    13981 acaaggtagg gtcttcctga aatgtcaaga gaaatattaa caagaacttc atccattgga
    14041 gttgaagaaa atccaaatct tttaattccc tttttatctc cgagtgcttc ttttattgct
    14101 gaaccaagac atattcctgt atcttcaaca agatgatgat tatcaacctc tatatctcct
    14161 tttgctttta tttttaaatc aaaatttgaa aattttgaaa ataattcaag catatgattt
    14221 aaaaaaccta tcggtgtttc aatttcagaa tccccctttc catcaagatt gatttcaatt
    14281 tcaatatctg tctcttttgt tttcctttta aattttttct ttctgggcat agaaatattt
    14341 tacaacattt ttaccaattt gaaaaatcat aatatctgaa agttattttt tgggggtcat
    14401 attccctaat ccatgagaca tgtccatcag caaacaatac atttaatcca ccaagatggt
    14461 atggtgccca gtggtcttct gtttcttccc aactcaaaca attctgagaa tagtcatcct
    14521 tatcattgtc attcacttca aaatttttat actcattatc tcctgcaaga acaaaagcac
    14581 tcggatatct tatttttttt ctgtttgttg ccgctctctg atttcctgca tcaatatatg
    14641 ctgctcttgc acttaaaaaa taagtaaatt ggtcattttt aacaggatag gtaggacatc
    14701 tgtaaatatt tttatttttt acataagaat agagttgttc tgtccagcca ggatatccaa
    14761 gatttggtgg tggactgata tttcctgttc cccagaaaca tctcccttga cagaaaggat
    14821 aaaactcttc attatcatta gcatacatct caagagcaac atataactgt tttaaattat
    14881 tcatgcaggt ggcttgtctt gctttttctc ttgctttact taatgcaggt aaaagcatac
    14941 cagcaagaat tgctataatt gcaattacca caagaagttc tatcaatgtg aagcctattt
    15001 ttttattttt catttttaat ttcctgttta tttaatagtt aaaaagttta aatctttttt
    15061 atttttaagt caaaatttat ttaagggttc ttcagaaatt tttcaacttc ttcttttcct
    15121 ggagatgatt gggcacctat ttttgtgact gtcaaagcag caacaatatt agcaaaataa
    15181 actgcttgtg tcaaatcttt cccttctgca atagcaaatg cgagtgcacc attgaaagca
    15241 tctccagcac ctgttgtatc aactgccttt actttttttg aaggtacaat ttcacttaaa
    15301 gtttttgtaa ctaaaaatac tcctctttct ccaagagtaa ctataactgt ctttatccct
    15361 ttgtgtagta atttataaga tgcttttttt attgaattga ttgaatttaa attttcgcct
    15421 gttaactttt caagttcaat tttatttggt gtaagaatat caatattttt aagtaaacta
    15481 tcagggattt ttttcgctgg tgctggattg agaataacag ggatattttt ctttttggca
    15541 agttttacag catattctac agtatcaatt ggtatttcca gttgaagaag aaggatatct
    15601 gaagaaaata taagtttttt atgtttttca atatcctttt tccctaattt gtaatttgct
    15661 ccaggattta ctactattga attttcacct tttttatcaa taataattaa tgcaatacct
    15721 gatggacttc cagggtcacg aataatagat tccgttttta tattctcttt ctttaaattt
    15781 tttataatta catctccaaa aaagtcattt ccaatacagg atataaaatt tacctttgca
    15841 cccaatcttg aagccacaac tgcctgattt gcaccttttc caccaggaca cataaaaaat
    15901 tgctttccaa aaacagtttc tccaggagat ggataatgcg aagttttgac caccagatca
    15961 atatttgaag aaccaataac agtaattaca ggagtccttt tcataagtat ttttctatta
    16021 actttctgat atctttaatt ctttttttat aatctctttc ttctctaaat atacttgttg
    16081 aacctaaaac aaatatatct gctccttctt taattaataa gggaatagtt ttttcattga
    16141 ctccaccatc tacctcaatg gataaatcag aattaacttt ttctgcaata tttttaattt
    16201 cctttatttt tttaattgat gaaggaatga aatttccacc tgcaaatcca ggttccacac
    16261 acataacaag aactgtatca atttctttaa taagttcttc aatacagcat acaggggttt
    16321 tagggtttat cgcaatgcct gtttttatat ttttattttt taactcattt aaaattctat
    16381 aaggataaag tactgcttca taatggacta ttataatatt gccacctgct tttataaatt
    16441 tttcaatcaa attttccggt tgataaacca tcatatgaat ttcaaaaggt aaaaatgttt
    16501 tttctctcaa tgattctact atatcaggac caaatgtgat atttggtaca aatttattat
    16561 ccataatatc aaaatgaaac gaatctgcac ctgcttcttc aagttttttc acttcttcac
    16621 caagttgtga taaatccgca cacattaagg acggtgaaat tttaattttc tttttcattt
    16681 ttatttttta tccttttaag aattaaccca aatttctcaa attgtcaaag taaaatactt
    16741 tttcagataa ggttggttta actatgattt tcttattttt attgaattct tatggctttc
    16801 aagtttttct acctctgcta ttttttctat gtatttaccg tactctttta taaattcttt
    16861 tttacatttt ataactgcaa aactccttaa aaaagtaaat acagaaagac ctgaagaaaa
    16921 gtttgctgac attcctgttg gtaatacatg agaaggacct gcaaaataat cacctattgt
    16981 tacaggtgta taattcccta taaatatcgc tcctgcctta caatttttaa agagaaaagg
    17041 tttttttgaa attacctcta aatgttctgg tgctatatag tttattattt caattgcttc
    17101 tttttcattt ttaacaagaa tccagtatcc tttttcaact tttttttcta tatttttgcc
    17161 aattttttca tctgttgtta ttaaaatccc agttccatca gtatgttcaa tctgtgcctt
    17221 taaatcatat tcaataaaat caggaattcc agaactatct gaaaaaataa caacttcgct
    17281 cggtcctgct ggtaaatcaa ttccgacctt tccaaataat aactttttag cacaatttac
    17341 atatttattc ccaggacccg ctattaaatc aaccttagga attgtctctg tcccaaaagc
    17401 aaaagcactt attgcacaca caccaccagc agaaaaaata tttttaattc caagataatc
    17461 acatgttgcg attattaaag gatgaatttt cccattataa gaaggtggtg agcagacata
    17521 tatttcctta acagttgcaa cttttgctgg aattactgtc attaaaattg ttgaaattaa
    17581 aggggattgc cctcctggaa tatatatacc aactttttca attggctgat acctgaattc
    17641 gatttcaata tatttttcct ttattttaaa tccttttggc aactgttttt tatgatattt
    17701 ttctattctt tcttttgctt ttttaatgta ttcaaaaatt tctcctttta agtattttct
    17761 gctttctttt atctcatcct cattcacctt taaattttca agttttactc tatcaaactt
    17821 ttcagtataa taaaatattg ctttatctcc ttcttcttta actcttttta taatttcctt
    17881 tacacctttt tctatatctt cttcttttct ttcaagtatt tttttcaatt cttcaaaact
    17941 tatcttttcc atcttaaact ttcaatcctt tctttatagt cattccctga aaaaatgtaa
    18001 ttaccagcaa caagtatatc aactccattt tctaccacaa tttttcctgt ctctttattt
    18061 ataccaccat ctacctcaat ttcaaaattt aatttcttat tctttctgta ttcattcact
    18121 ttttttattt tttcaactac ctcatatata aatttctgtc ctccaaatcc aggattaaca
    18181 gtcataatca aaaccatatc aaccatttct aaaacaggat aaattttctc aaccggagtg
    18241 ggtgggttta ttacaataca tgctctttta ccatatttct gtatttctgt aattatgtat
    18301 ttatggtcaa cttcacattc tatatgtatt cctattatat ccgctccact atcagcaaaa
    18361 tttttccagt atttttcagg gtttgttatc attaagtgtg cctcaaatgg tagatgtgaa
    18421 tatcttctta ttgcagcaag aaccattgga ccaaaagtta aatttggaac aaaatgtcca
    18481 tccataacat caaaatgaat caaatcagca cctgcctctg taactttctt cacctcttct
    18541 cctaaacgag aaaaatcagc agaaagtaaa gaaggagcaa ttttaaccat agaaaaattt
    18601 tagcataaaa attattttat tgaaaggaga gagccatttt caagaagata tattttattg
    18661 cagacccttt caagaaaatt tatatcatgg cttaccatta aataggtaag attattctga
    18721 attaaaaatt tttcaattat ctggcttgtt ttttcatcaa gacctgttgt tggctcatca
    18781 aggagatata tttcaggttc cattgataga attgttgcaa ttgctacaat tcttttttct
    18841 ccataagaaa gtgtatggga aaatctgtgt tttaaattct ctataccaag aagttttata
    18901 gtcctttcaa ccctttcttt tacttcttcc tttggtaatc ttaaatttaa tggtccaaaa
    18961 gcaatatctt cttcaacagt aggagaaaat aactggtcat cagagtcctg aaacagaaaa
    19021 ccgatttttt ttcttacctc aacaaaatca ctctcttttt ccatcttttt accgaatatt
    19081 tttatctctc ccttttctat ttttacaaga ccaacaattg tataaagtaa ggatgttttt
    19141 ccggaaccat ttgctccttt tattccaatt ctatccttct ctgaaattga aagggaaaga
    19201 ttatcaagga cttttcctct ttcaggatat gaaacagtta tatttttaag ttcaatcagt
    19261 ggatttttaa aattattatc catagaaaat aaattataac acagaaacta acgaatgtat
    19321 ccttttttga ccatacaaaa tgatgatatg tccagaatat tccggaaaag ttccttaaaa
    19381 tcattgcttt ataaattctt tcacttcttt catatgacct taaaagtaaa attcctacaa
    19441 aatttcctat tgttttatat gtgtggatat tatttttagg gacaaaacat cttattttca
    19501 ttgctttttg aattctattt ttttctttct ctatcacagg aatatatctc gttgaaagaa
    19561 agaagatatt aataagtttt ttgggtaaat gaagatgatg caaggcatga ataattctta
    19621 tgggatgggt tgaaaaaatt aaaacaaaat ttgttaaaaa gattagattt gccttaattg
    19681 taattaaaag agtatatctt attccttcat aggtcaccga aaatttcaaa aagcggaaaa
    19741 cttcttttcc aggaatactt aaaggtagaa aaagccagca tagaaatata aaaagattta
    19801 cagaaataag ccctttaaaa aattttttaa aatctgtgat aaaaaaaaat aaaattactg
    19861 gcagaataag gtaaaattta aaaatttcaa aatttctttc aagagcaaca aatatagagt
    19921 aaacaaaaga agttaaaagt cgtaatctag ggtctatttt ttccattctg aggaccgtcc
    19981 tcggaaataa aggtaaatcc ctaatatgcc aaaaatataa ccaatccctc caattatatc
    20041 tgtcaatttt attttctgtt tttctttttc tatttctttt ataagggggt ttattgcttt
    20101 ttcaacactt tcttctacta tctcttttat tttttcttca tcaattcctt tttgtatttc
    20161 agttttttct tcctttttct ctaccttttt ttcttctttt ttaatttcag tttttttctt
    20221 caattccatt tctgtttcta ctttatgacc catatcagcg gtaagaacaa ttttaatttt
    20281 ttctgcatcc ggaatatcaa aagagaaaat gcctttttca tcagtttttc catcaagaat
    20341 tttttcactt ttttcgttat atacctcaac aggtgaattt ttacatggag aaccatcctg
    20401 aaaatatccc tcaacaaaaa ttttatttcc ttccttataa gtaaaaatat ttattttgtg
    20461 agcaaaagta atgactgaaa aaataaaaaa tataaaaatt aattttttca tttatacctc
    20521 cagtaattcc ttttttgttt tacttaaaaa agaaagaata aagccggtta taaaaccttc
    20581 aataactgca attggtagat gaactacaaa aatagttttt ccaacatttt caaaaaaact
    20641 acctgtaaaa taaagagaga aaaatacaaa aacacatgat agaataattg aaaaaaagcc
    20701 tgaaagaaaa cctgttaaaa aataattttt ctttaaaaat ggtttgaaaa tataataaga
    20761 aattaccgca ggaatcgcca tatttgccgt atttattcca agtgttgtaa gacctccaaa
    20821 ttgaaataat aatgcctgta agaataaagc aagtaagata caaggaaaac ttttccagcc
    20881 aagaattatt ccgcaaaggc catttaaaac aagatgagaa cttgaaggac ccacaggaac
    20941 atgtaccagt gatgcaacaa aaaaagcact tgttaataaa gagacctttg gataatcctt
    21001 atattctaaa tctttaagtc ctttatatgt ccctgccaaa gttattacag cgcctattcc
    21061 aagaataggt ccggacaaaa ccccttctga tatatgcatt ttatttcatt tcataaactt
    21121 taatccacag tactcctgca acttcaactt ctttatcatt tatctttttt tcatctgtac
    21181 aaattgctga aaatccatac catccacttt taggaaaaga ataatagaat acgccatttt
    21241 ggtctgtttt aacaacttga gttataaatg gtgaatatgg tgcttttata tttccctttt
    21301 cattaaaata ctcaatttct acatcacaga aaggaacggg ttttccatta aatttaacaa
    21361 ttccataaaa ggtattacca gcataaattc cataaggtct gacaagaggt actatttcaa
    21421 cttttagtcc tatttcctta tcccaactat cttccattcc aaatccatta acaataactt
    21481 ttgtatagtg aattatatat ttttcttcag caggttccca gtaaggcgaa ggaatacagt
    21541 aaaaaatata atctccgggt cttttcaaag tataacttaa actccaacct ctgtgtatct
    21601 tttcatcaga gtaaaattta taacttgttt ctttaatctc atttaacaat gaaatctttt
    21661 ctcctctgaa aaatactcca aaatcagatg gtttttccat atccattgtt tttccttcaa
    21721 aaggatgtgc aaataaaatc tttaaattta tatttgcttt ttctttctcc tcaacaatgt
    21781 caggagaagg tataatcatt ccaaagtgag caaaagaatt taaaaaaatt gaaagaaaaa
    21841 gatataaaac taatttctta ttcataattt cctccctttt accagtttaa agttattcct
    21901 gttgttattc tccagtcagg tgta
//
LOCUS       DAOSAT010000018        20536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_1844_length_20536_cov_4.698501, whole genome shotgun sequence.
ACCESSION   DAOSAT010000018 DAOSAT010000000
VERSION     DAOSAT010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20536
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1844_length_20536_cov_4.698501"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PLF90_02705"
     CDS             <1..1054
                     /locus_tag="PLF90_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56228.1"
                     /translation="VDIRLAGVSRHLYEPERKVYFLGMEGELFEGDVYTLKVNFLFDL
                     NKILEIEGRPHFKDIYCDHGRVVVVNNSYYYEDFERGYSDGRLAEWDGKNWRIIERTQ
                     FNTLAGRCRGNLGRSIFAVGHDKASILFYVYLPQTDWVLYRLPRATHTQDHAWTTEWP
                     RIREVESERWLMDAGGMFYELPVMTYANKVWGVIPICRHLRIIPDFCSWNGFLVLGGN
                     QATPINDDNPFVGQPQANLWFGKTDDLWQFGGKPAGWGGPWWDKKVNEGEFSEPFLIY
                     GFEKKCLHISHKERSRKKFILEVDFRGDGIWKKFTEIETKSSYAFYDFPDGFSAHWIR
                     IVSFDKVTVTAQFFYN"
     gene            1072..2232
                     /locus_tag="PLF90_02710"
     CDS             1072..2232
                     /locus_tag="PLF90_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOK56229.1"
                     /translation="MEYFLTEEQKMIKELARKIAEEKIKPKRAEYDENETFPWDIVEE
                     LAKSDLFGVFVPSEYGGLGYGVFELCLVIEELSRVCGGIALAYAGTALGTFPIVLFGN
                     EEQKKKYLPPIAKGEKLAAFAVTEPEAGSDITNLNTIAKKEGDYYILNGTKQWITNGG
                     EAEIYVVIAATDKTKGPRGATAFILEKGMEGFNFGKKEKKLGIRASATRELIFENCKL
                     PKGNVLGREGMGFLITLKNFDTSRPGVAAQALGIAQGAFDEALKYAHTRKQFGQTIIS
                     FQAISHMLADMATQIEAARCLIYQTAKMIDSGAKEFSKESAMCKLFASDVAMKVTTDA
                     IQILGGYGYMKDYPVEKMFRDAKITQIYEGTNQIQRNIISLELIKELTRNKP"
     gene            2236..3120
                     /locus_tag="PLF90_02715"
     CDS             2236..3120
                     /locus_tag="PLF90_02715"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOK56230.1"
                     /translation="MREIRFDEEGKVRVDKYLKEITGLSRERIKNLIRNKKIFVNGKV
                     IEPSYILQKGNLITIFEEEIDKEFNIEPEKGQLDIIYEDEDIIVLNKKAGILTHPTHA
                     IKSGTLVNFLLYHTKLSNIGSPYRPGVVHRLDKETSGVIVFAKTDYAYWCLIEQFKNR
                     IVKKEYYAIVEGRFPEKKKVVEFRVSPDRENPTKMKVHFLKGKDALTEIEVEKYVGNY
                     TLLKIKPVTGRTHQIRLTLSYLGYPILGDDKYGKKTEIIKRVALHAYQISFLHPKTKQ
                     YVEFSAPFPEDLKKILTV"
     gene            3158..4069
                     /locus_tag="PLF90_02720"
     CDS             3158..4069
                     /locus_tag="PLF90_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK56231.1"
                     /translation="MEDLKIGIIGFDTSHVVAFTKLLNDENDPFHVKGGKVIAGYPSF
                     SPDIQASYSRVEGFKKELVEKYNIEIVNTIEELIEKVDAVLLESVDGRRHLKEAETVI
                     KSKKTLFIDKPLAANYSDAKKIYKMADEYKCPVFSSSSLRFDYNFTKIKNDINIGEIL
                     GCDAFSPCTLESTNPGFFWYGIHGVEILYTFMGKGCRKVYCEKTEDFHFATGIWDDNR
                     IGSVRGIRKGFLNYGATVFTEKKVIHVTYSTEIPIYAQLLKEIIKFFITGKPPVEKEE
                     TLEIMKFMECALISEKEKRTVNLSEID"
     gene            complement(4095..5366)
                     /gene="rho"
                     /locus_tag="PLF90_02725"
     CDS             complement(4095..5366)
                     /gene="rho"
                     /locus_tag="PLF90_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOK56232.1"
                     /translation="MNVSTLRKLSMTKLQKIAKEMEVNGISGLRKEELIYKIMEAQAK
                     KNGLLFGEGVLEVLPDGFGFLRVPNYNYLPGPDDIYVSPSQIKKFGLKTGDTVSGQIR
                     PPKENEKYFALLKVEMVNGEPPENISSRIPFEELTPIHPDKRFILETTPEEITTRVID
                     LITPIGKGQRGLIVSPPRAGKTVILQKIANAIAKNHPEVYLIVLLVAERPEEVTEMRR
                     IVKGEVISATFDESPERHTQVAEIALEKAKRLVEHKKDVVILLDSITRLARAYNVLVP
                     HSGKIMTGGLESTALDKPKRFFGAARNIEEGGSLTIIGTALIDTGSKMDDVIFEEFKG
                     TGNMEINLERKLADRRIFPAIDINRSGTRREELLIDPEELQLIWLLRKILAPLNPIEA
                     MELLINKIKTTKSNIELLLSLRAQAKEYSNY"
     gene            complement(5399..5998)
                     /gene="coaE"
                     /locus_tag="PLF90_02730"
     CDS             complement(5399..5998)
                     /gene="coaE"
                     /locus_tag="PLF90_02730"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOK56233.1"
                     /translation="MKKSIIVGITGILGSGKTTITEILRKWKFKVISCDEIVHNLWKR
                     NNILKKIKKIFGEDIEKDGRINKEKVREIIFTDAIKKRQLENLLHPMVFDEINKNIFD
                     TKKNGGIIFIEIPLLFETNSEKLFDKIIVVSAPQKKIKERLMDKYSEKDIKNIWKNQL
                     PLALKKKKADYIIDNSGSIKKTEENLKRIVDELLKNLNQ"
     gene            complement(5995..7245)
                     /locus_tag="PLF90_02735"
     CDS             complement(5995..7245)
                     /locus_tag="PLF90_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOK56234.1"
                     /translation="MEYSIKDINLYREGENKVNWALQHMPVIKYLRNKYSRRKIFKGY
                     KIGACLHITSETANLIINFKECGAEVFLCASNPLSTQDEVAAYLVKKGIPVFGKKGES
                     GSEYYKNIENVAKNMPHFIIDDGGDLITFVHQNEKYIKNIIGATEETTTGVIRLKNLN
                     KQKLLKFPVIAVNNAKTKYLFDNRYGTGQSTVDGLLRATNILIAGKIIVVCGYGWCGK
                     GIARRFRGMGGRVIVVEVDSIKALEAVMDGFLVMSMKEASKIGDIFITATGNTSIITM
                     KEINKMKDGVILGNSGHFNVEIKMDEIEENTLRKKRIRNGLTEYILKNGKKIFILGEG
                     RLLNLACAEGHPPEVMDMSFANQFLSIKFIKEHPNLKVNVYDVPEEIDKQIAKYKLNT
                     MNIKIDSLTDLQERYLSSWELGTL"
     gene            complement(7253..8395)
                     /gene="metK"
                     /locus_tag="PLF90_02740"
     CDS             complement(7253..8395)
                     /gene="metK"
                     /locus_tag="PLF90_02740"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOK56235.1"
                     /translation="MKNYYLFTSESVCEGHPDKICDQISDSILDEVLKQDKNGRVACE
                     TLTTRGLLFIAGEITTTGYVDIPELARNLLKEIGYIDPSYGFQYESVGIITSIQEQAP
                     EIATGVDKGGAGDQGMMFGYATDETPELMPLPITLAHKLVMRLAEIRKKKIVDYLRPD
                     GKSQVTVLYENEKPVSVESVVVSAQHNPGVSQKKIKDDIIELVINYVIPEELRSKKMK
                     IFTNPTGSFIIGGPMADTGVTGRKIIVDTYGGVGSHGGGCFSGKDPTKVDRSGSYMAR
                     YLAKNIVASGIARKCEIQIAYVIGQVEPVSLMINTYGTGLIPDSEIVKIIKDNFDLSP
                     RGIIKKLDLLRPIFRKTACYGHFGRNDEDFTWEKTDSKEIFTKNIR"
     gene            complement(8392..9429)
                     /locus_tag="PLF90_02745"
     CDS             complement(8392..9429)
                     /locus_tag="PLF90_02745"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOK56236.1"
                     /translation="MELKKSLMEKYDKSDMFSLLKNFPRHCLEGYNLTISEINKNFEK
                     VVFTGMGGSAISGDIIKILIEEIAEIPVFVIRDYSLPKYVDSKTLVIVESYSGNTEET
                     VNTYKIAKGNKSLIICISSNGEIEKMACSDGIPLIKIPSGMPPRCAFGYLFFPVYRYF
                     VDAKILPPLEKTFFEKIEKWVNEFLPSSENNKAIEISKKIYKNIPLIYSENRFYPGIL
                     RWKTQIAENSKNFAFVNILPEMNHNEIMAFYFPEFFIKKAICLFIVSGIENERVKKRI
                     EITSNIISKKVKEVMELKLEGETLLEKTMYLIILGDWISYYLAILNKVDPTEIKEINI
                     LKEEMKKGGKK"
     gene            complement(9443..11149)
                     /gene="ptsP"
                     /locus_tag="PLF90_02750"
     CDS             complement(9443..11149)
                     /gene="ptsP"
                     /locus_tag="PLF90_02750"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOK56237.1"
                     /translation="MKKIKGIPASPGFAIGKARKVNDDFFSFSEIKKIKEEDVDEEIK
                     RFHESITKTENEIQQIINNIKKITDKEYAEIFSFHLSILRDKNLMERIEKIIKRERVS
                     AETAVKKFVVNLNKEFSENNLKTRKKEILDIFERIIANLIKKNDRLKDIKDGILVAND
                     LSPTQTVSIDKKYIKGFVISMGTETSHTAIVAKALEIPAVVGIKENIEEIKDGDTILI
                     DGYEGTVIVNPTQIEIEKVKSKEKEIRKFRKKIYILKGKKTITSDGKEINLYANIQLP
                     EEVETALKYGAEGIGLFRTEYLYLKRKDLPTEEEQFLSYKKIIEKFGNKEIIIRTIDV
                     GGDKFISAFQGPKELNSFLGLRGIRFCLEKRDVFLTQIKAILRAGYYGNLKVMFPMIT
                     TKEEVIEAKKIIECAKKELRKENLKFKENIQVGIMIETPSAALVSDDLAKEVNFFSIG
                     SNDLIQYTLAIDRVNEKLSYLYQPCHPSVLKLIKITAENAIKNNIEVSLCGEMASIPE
                     IACLLIGLGIKKLSMAPVAIPSVKEKIIKNSYTEMKEIAEKVMNFNKHEEIINFLRKN
                     LK"
     gene            complement(11162..11635)
                     /locus_tag="PLF90_02755"
     CDS             complement(11162..11635)
                     /locus_tag="PLF90_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOK56238.1"
                     /translation="MKIVDYLKEDCIITNLKSRDKKGVLREIIKTLEKNGYVKDSEKI
                     LETVMDREKLGSTGIGQGIAIPHAKTDQINDIICALGLSQTGVDFDSLDGEPVYIVFL
                     VLAPTKSIGQHLKTLAKIARLLKDKVFRNALRNCKTPEEAIKIIKEDEEKLNSLS"
     gene            complement(11652..11936)
                     /locus_tag="PLF90_02760"
     CDS             complement(11652..11936)
                     /locus_tag="PLF90_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOK56239.1"
                     /translation="MDIKIYSEVEINKEFEDYIKEKIEKLKKFVFDDGYAEFYIKKDG
                     PLFLTEIYLHSKNIKVFLKEKDNDVNKSVENLLDKAKVKLRKLHDKIVNK"
     gene            complement(11953..12852)
                     /locus_tag="PLF90_02765"
     CDS             complement(11953..12852)
                     /locus_tag="PLF90_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOK56240.1"
                     /translation="MLKLYLKKKFLNNVFLFFMCINYFIFAEEETIFIKLLEGNTVKE
                     IEIEEYISGVVVKEMGENWPVEALKAQAVVSRTYLIWKISTNKRNYYHIENSINHQLY
                     KDCKDEKIKSILLETKGEVLLTENEKIVPVFFHACSGGITANPSDVWKGKYPLNYSVI
                     DPYSEDNPFSYWEKNISKNYLSKILGCSIEKIEVIERDQTNRVKLLQITTKNGNIKLR
                     GTEFREKINQKTEVYFNNPYIIPSTLFEVRDKGDSITFIGKGYGHGVGLSQYGAKKMA
                     EIGFDYKDILKFYFPGLKIKKIY"
     gene            complement(12815..13381)
                     /locus_tag="PLF90_02770"
     CDS             complement(12815..13381)
                     /locus_tag="PLF90_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56241.1"
                     /translation="MKKIIKNLILFSFMFFIFVSCTKKKNEEIISKDEKIAEFSLKHF
                     SKNYSITLIGESAEITENKNTKISAPNLSLKTDKEIIEIKTDKEGKGEIKIDPENKKL
                     KEIMITGKVIILYKDKTTENVTMEITCGKVSYIDEKREMIFEEKPLIKRGKNKFSGEK
                     VIYNIDNNTIEIKGNVHVEIVPEEKVSK"
     gene            complement(13386..13721)
                     /locus_tag="PLF90_02775"
     CDS             complement(13386..13721)
                     /locus_tag="PLF90_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020581475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOK56242.1"
                     /translation="MNCVFCKIINNEIKSDKIYEDDDMVCIRDINPQAPVHILIIPKK
                     HIEKLYEVEDTFLLGKMLKIACQIAEKEGIKDSGFRIVINTNRNAGQSVDHIHVHLIG
                     GRIMMWPPG"
     gene            complement(13725..15725)
                     /gene="infB"
                     /locus_tag="PLF90_02780"
     CDS             complement(13725..15725)
                     /gene="infB"
                     /locus_tag="PLF90_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOK56243.1"
                     /translation="MRIYELAKKYNLQTRDLLKVLENIGITGKQTLSVITDEEIKKID
                     DYFEKDKEKGEKSEGKKEIIEEKGKPEKEEKLKEEVIKKEIILTGKENIKELSEKIQI
                     QSTEIQKFLLEQKIIVNINQTLRKDIIQKICENFNFIPVFKEEQQPKILEKTEKEEKP
                     IYVKRAPVVTVMGHVDHGKTTILDKIRNSKVAEKEFGQITQKIGAYKVNLPEGSIVFL
                     DTPGHEAFTAMRAMGAKITDIVVLVVAADEGVKPQTIEALDHAKSANVPIIVAINKID
                     RPNANPEKVKQQLSEYGLIPEEWGGNTVYVNVSGLTGQGINDLLEMILLVGEMLELKA
                     IINCKCNGTVIESSVDKFRGPVISVLIENGKLKVGDPFIVGSTWGKVRAIIDEWGKRL
                     NEAGPSTPVEILGAHEIAIPGEKFRVVDSEKEAKDFSEKLKREKESIVIPVKRLTLKD
                     LYEEIKKGEVKELNVILKTDFYNSIDAIKNVFNKIPQNEIKINIIHAGTGPISESDVL
                     LASASNGIVLGFKVPVEPKAKELAKKENIEVRTYELIYDIGDDIIKAIEGMLEPEIKE
                     EMVGQALVKKVFKLAKNNIVAGCVVVSGKVVKNSKIKIVRNGKVIFEGNLTSLKRFKD
                     TVNEVNYNNECGIGIDNFYEFQEGDIIQSFITIEVPRKLKGG"
     gene            complement(15730..16695)
                     /gene="nusA"
                     /locus_tag="PLF90_02785"
     CDS             complement(15730..16695)
                     /gene="nusA"
                     /locus_tag="PLF90_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOK56244.1"
                     /translation="MRKEIISLLEYWEKERGIERNHLIESLEDGLLTVYRKKAQLPKH
                     IKIKINPETGDIKFINENGEEVPPPSFPWERIAAQTARHVLIQKIKEAEKSTIYNEFK
                     NLEGNIISGRVERFENDNVIVVTGKAEGVLPHHHKLPNDHFKLGDGIKCYLIEVRPPL
                     KGNYQLIFSRTHPNFVKKLLENEIPEIKEGIVEIKAIARFPGDLTKVAVFSKNEKIDP
                     VGTCIGDKASRIKNIVKELRGEKIELILWDEDIAKYIKNSLSPAKCNEVHIDEEKKEA
                     VAIVDDDQLFLAIGKKGQNVRLASKLTGWNIKVFKVSEYKKETNI"
     gene            16737..17930
                     /locus_tag="PLF90_02790"
     CDS             16737..17930
                     /locus_tag="PLF90_02790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK56245.1"
                     /translation="MSSNVKLKHMKNKSIFLILLFGIISLFGDIVYEGVRSISGPFLA
                     SIGASAVIIGFFSGLGEFLTYFLRFFSGYISDKKRIYWPFTIIGYSLICCLPLLAFTK
                     IWQIAVILLLLERIGKAIRTPSRDTLISFAGKDIGYGKSFGIHEVLDQIGAILGPLIF
                     FFSLSRFKSYSVGFKFMWVPSFFLILTLIFARKNYNIIVEKEEKINFLQKNNLNQNFT
                     FYLLFVFFSLSGLITFPIISYHLSTKKIVIFSGIPVFYIIAMSVDGIVALFIGNLYDK
                     IKLKTLLLIPFLTIVLSFSVLSTNIFFSLIGIIIFGVIIGCHETILRASIADMIPSFQ
                     RGFAYGIFNTIYGIALFIGSWIIGFLYGKNFKLIWVYVIIMEIISFVFLFYQIKIKNV
                     VEKAR"
     gene            17908..18273
                     /locus_tag="PLF90_02795"
     CDS             17908..18273
                     /locus_tag="PLF90_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56246.1"
                     /translation="MLWKKHGKFELGKNKVEIRSIKLKDEIGKIRRLRICSVWSNFSK
                     FTKIPGEGCLCKDEKGYIGVLLTGKNGAYIKVGKKFLISQNLIVPFNSISKTSLKKLL
                     KGYNIDFFENGDAIYGLEK"
     gene            18270..19661
                     /locus_tag="PLF90_02800"
     CDS             18270..19661
                     /locus_tag="PLF90_02800"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOK56247.1"
                     /translation="MKKKQIITPEEIINKMRDFCFEKKINIFLVGGYLRDKFLKKKSK
                     DIDFVMEEDALKIAEEFSKRFNLPAPVFYGRFGTAMIEIENIKLEFATARKESYFENS
                     RKPVVEKCSIFEDLARRDFTINAIAQNLITGEILDPFEGRKDLKNKIIRTPVEPDKTF
                     FDDPLRILRGIRFATKFKFKIDEKTLEGMKKNKDRIKIVSEERIADEIMKILESSTPS
                     IGFKLMDEIGLLEIILPEVANLKEKRTDHPCKELFPHTLKTLDNISKYTKNVYLRLAA
                     LLHDIGKPYTLAVEDGKVSFHRHEFVGEKMAYNVCRRLKIGEPEIRFIGRLIKFHLRP
                     HLLAKENPSDSALRRFIKEIGKDIKPLFILAKSDLTSRNPLKIQEALERLERLETRIK
                     EINKKDKLSSFKLAINGFDIMEILKITPGKKVGIIKEYLENLVIEGQLPNKKRILRRY
                     LIENVEKLLNLNE"
     gene            19663..>20536
                     /locus_tag="PLF90_02805"
     CDS             19663..>20536
                     /locus_tag="PLF90_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOK56248.1"
                     /translation="MRIGIDISSILSKRKTGIGWYTYYLIKNLIEVDKENEYVLFGCS
                     IGNYRGKFNLKGDFNGENLNISVKFLPNKLMSSLHIFFPIEFLHGRFDVIHITLHPYS
                     VLNIFGKQIVTVHDLCHLINSKWFHPVESYPFKIVITKTVQRVDRIIAVSESTKRDLI
                     EYLGVKSEKVEVIYEGVDEIFRVVEDREKIEEVKRRYGISKRYILTVGTIEPKKNHIR
                     LLNVFKRIREKREDYQLVICGKYGWKTERFLERLSGLPDFIRKDIIITEYVRYEDLPL
                     LYNGCDIFIYPSLYE"
BASE COUNT         6609 a         3100 c         2848 g         7979 t
ORIGIN      
        1 tgtagatata cgacttgcag gtgtttcaag acatttatat gaacctgaaa gaaaagttta
       61 ttttcttggt atggaagggg aattgtttga aggggatgtt tatactctca aagtgaattt
      121 tttgtttgac ttaaataaaa tccttgaaat tgaaggtaga ccacatttca aggatattta
      181 ctgtgaccat ggtagagttg tggtagtaaa taatagttat tactacgaag attttgaaag
      241 aggatacagt gacggaagat tagcggaatg ggatggaaaa aactggagaa ttatagagag
      301 aacacagttt aatactcttg caggaaggtg tagaggtaat ttaggtagaa gtatctttgc
      361 tgtcggtcat gataaagcat ctatactttt ttacgtttat ttacctcaaa ctgactgggt
      421 tttatataga ttgccaagag caacacatac acaggaccac gcatggacta cagaatggcc
      481 aagaattaga gaagttgaat cagaaagatg gttaatggat gctggaggta tgttttatga
      541 attacctgtt atgacatatg ctaataaagt ttggggtgta attcctatat gtagacattt
      601 aagaattatt cctgatttct gttcatggaa tggtttttta gtccttggtg gaaatcaggc
      661 aactcctata aatgatgata atccttttgt gggacagcca caggcaaatt tatggtttgg
      721 taaaacagat gatttatggc aatttggggg taagcccgct ggatggggtg gtccatggtg
      781 ggataagaag gtaaatgaag gagaattttc agagccattt ttaatttatg gatttgaaaa
      841 aaaatgtctt catatttctc ataaagaaag gagtagaaaa aagtttatat tagaagtaga
      901 ttttagaggt gatggtattt ggaaaaagtt tacagaaatt gagacaaaat caagttatgc
      961 tttttatgat tttccagatg gtttttctgc ccactggata aggatagttt catttgataa
     1021 agttacagta acggcacaat ttttttataa ttaattggaa aaggagtcaa aatggaatat
     1081 tttttgacag aagaacagaa aatgattaaa gaacttgcaa gaaaaattgc agaagagaag
     1141 attaaaccta aaagagcaga atatgatgag aacgaaacat ttccttggga tattgtagaa
     1201 gaacttgcaa aatctgattt gtttggtgtt tttgttcctt cggagtatgg aggacttggt
     1261 tatggtgttt ttgaattatg tcttgttatt gaagaactga gtagggtatg tggaggtatt
     1321 gcacttgctt atgctgggac tgctcttggt acttttccta ttgtactttt tggtaatgaa
     1381 gaacaaaaga aaaaatattt accgccaata gcaaaaggag aaaaactggc tgcatttgct
     1441 gttacagaac cggaagcggg aagtgatata acaaatttaa atacaattgc aaaaaaagaa
     1501 ggtgattatt atattctaaa tggaaccaaa caatggataa caaatggagg ggaggcagag
     1561 atttatgttg taattgctgc aactgataaa acaaagggtc caagaggagc caccgcattt
     1621 atacttgaaa aaggtatgga aggttttaat tttggaaaaa aagaaaaaaa acttgggata
     1681 agagcatctg ctacaagaga acttattttt gagaattgta aattaccaaa aggaaatgta
     1741 ttgggtaggg aaggtatggg ttttttaata acacttaaaa attttgatac aagcagacct
     1801 ggtgttgcag cacaagctct tggtattgca cagggtgcat ttgatgaagc acttaaatat
     1861 gctcatacaa gaaaacaatt tggtcagaca attatatcct ttcaggcaat cagtcatatg
     1921 cttgcagaca tggcaaccca gattgaagca gcaagatgtt taatttacca gacagcaaaa
     1981 atgatagata gtggagcaaa agagttttca aaagagtcag cgatgtgtaa acttttcgcc
     2041 tcagatgtag caatgaaagt tacaacagat gcaattcaaa tacttggtgg ttatggttat
     2101 atgaaggatt atccggtaga aaaaatgttc agggatgcta aaattactca aatatatgaa
     2161 gggacaaatc agatacagag aaatataata tctcttgaac ttataaagga acttacaagg
     2221 aataaaccgt aataaatgag agaaataagg tttgatgaag aagggaaagt tagagttgat
     2281 aaatatttaa aagagattac aggactttct agagaaagaa taaaaaatct cataagaaat
     2341 aaaaaaattt ttgtaaacgg aaaagttatt gaaccttcct atattttgca gaaaggaaac
     2401 ttgataacaa tatttgaaga agaaatagat aaagaattta atattgaacc tgaaaaaggt
     2461 caacttgata taatttatga agatgaggat atcattgttc taaataaaaa agcaggaatt
     2521 ttaactcatc caacccatgc gataaaatct ggcacacttg ttaatttcct tctatatcat
     2581 actaaacttt caaacattgg ttcaccttat agaccaggtg ttgttcaccg tcttgataaa
     2641 gaaaccagtg gagttattgt atttgcaaaa actgattatg catactggtg tcttattgag
     2701 caatttaaaa acagaatagt taaaaaagaa tattatgcaa ttgttgaagg aagatttcct
     2761 gaaaagaaaa aagtagttga attcagagtt tctccggata gagaaaatcc aaccaaaatg
     2821 aaagttcact ttttaaaagg aaaagatgcc ttaacagaaa tagaagtaga aaaatatgtt
     2881 ggaaattata cattactaaa aattaaaccg gttacaggaa gaactcatca gattcgttta
     2941 acactttctt atcttggata tcccatttta ggagatgaca aatacgggaa aaaaacggaa
     3001 ataataaaaa gggtagctct tcatgcatat caaatttcat ttctacatcc gaaaacaaaa
     3061 caatatgttg aattttcagc accatttcct gaagatttaa aaaaaatttt gactgtttaa
     3121 agattaaatg tttatattta agaaaaaggg aggaaaaatg gaagatttga aaataggaat
     3181 aattggtttt gatacttctc atgttgttgc atttacaaaa cttttgaatg atgaaaatga
     3241 cccattccat gttaaaggtg gaaaagtaat tgcaggttat ccttctttta gcccagatat
     3301 tcaagcaagt tattccagag tagagggatt taaaaaagaa ctggttgaaa aatacaatat
     3361 agaaatcgta aatacaattg aggaactaat tgaaaaagtt gatgctgttt tacttgagag
     3421 tgttgacgga agaaggcacc tgaaagaagc ggaaactgtt ataaagtcaa aaaaaacatt
     3481 atttatagat aaaccactgg ctgcaaatta ttctgatgca aaaaagattt ataaaatggc
     3541 tgatgaatat aaatgtccgg ttttttcttc ttcctcttta aggtttgatt ataattttac
     3601 aaaaataaaa aatgatataa acattggtga aattttaggt tgtgacgctt tttctccctg
     3661 tactcttgaa tctacaaatc caggtttttt ctggtatggg attcacggag ttgaaatttt
     3721 atatacattt atgggtaaag gttgcagaaa agtatattgt gaaaaaacag aagattttca
     3781 ttttgctact ggaatatggg atgataatag aattggaagc gttagaggaa taagaaaggg
     3841 ttttttaaac tatggtgcaa ctgtttttac tgaaaaaaag gtaattcatg taacatattc
     3901 aactgaaatc ccaatttatg cacagctttt aaaggaaata attaaatttt ttataactgg
     3961 aaaaccacct gttgaaaaag aagaaactct tgaaattatg aagtttatgg aatgtgcgtt
     4021 aataagtgaa aaagaaaaaa ggactgtaaa tttaagtgaa attgattaaa tttaaaaaaa
     4081 ttttgttctg taaatcaata atttgaatat tcttttgctt gagcccttaa acttaataaa
     4141 agttcaatat ttgattttgt tgtcttaatt ttatttatta gaagttccat tgcttcaatt
     4201 ggattaagag gagcaagtat ttttcttaat aaccatatta actgtaattc ttctgggtca
     4261 ataagtaatt cttctcttct tgtgccagac ctgtttatat ctattgcagg aaatattctt
     4321 ctatcagcaa gttttctctc aaggtttatt tccatatttc cagttccttt aaattcttca
     4381 aatataacat catccatttt acttcctgta tcaattaaag cggtccctat tattgttaaa
     4441 cttccacctt cttctatatt tcttgctgct ccaaaaaatc tttttggttt atcaagagca
     4501 gttgattcaa gtcctcctgt cattatttta ccactgtgtg gaactaaaac attataagct
     4561 cttgcaagac gggttatact atcaagtaaa ataacaacat cttttttatg ttcaacaagt
     4621 cgttttgctt tttcaagagc aatttcagca acctgtgtat gcctttctgg tgattcatca
     4681 aaagtagcac ttataacttc tcctttaaca atccttctca tttctgtgac ttcctctggt
     4741 ctttcagcaa caagtaaaac tattaaatat acttcaggat gattttttgc aattgcattt
     4801 gctattttct gtaaaataac tgtttttcct gctctcggag gagaaactat aagtcctctt
     4861 tgcccttttc ctataggagt aattaaatca ataactcttg ttgttatctc ctctggtgtt
     4921 gtttccagta taaatctttt atcaggatga attggagtta attcttcaaa tggtattctg
     4981 gaagaaatat tttcaggagg ttcaccattt accatttcaa cctttaaaag tgcaaaatac
     5041 ttctcatttt cttttggagg tcttatctgt ccggaaaccg tatctcctgt ttttaatcca
     5101 aattttttta tttgtgatgg agaaacataa atatcatctg gtcctggaag gtaattataa
     5161 tttggtactc ttaaaaatcc aaaaccatcg ggaagaactt caagaacacc ttctccaaaa
     5221 agtaatccat ttttctttgc ctgtgcttcc attattttat atattaattc ttcttttctt
     5281 aaaccactga ttccatttac ttccatttct tttgcaattt tctgtaattt tgtcatactc
     5341 aattttctta aagttgaaac attaatttca ttagtttgat ccattctttt ctcctttttt
     5401 attgatttaa attttttaat aattcatcaa caattctttt tagattttcc tctgtttttt
     5461 taattgagcc agaattatca attatgtaat cagctttttt ctttttgaga gcaagtggta
     5521 gttgattttt ccatatattt tttatatctt tttcagagta tttatccatt aacctttctt
     5581 ttatcttttt ttgaggtgca cttaccacaa ttattttatc aaataatttt tcactatttg
     5641 tttcaaacag taggggaatt tctataaata ttatacctcc attttttttt gtgtcaaata
     5701 tatttttatt aatctcgtca aaaaccatag gatgtagtaa attctctaat tgcctttttt
     5761 ttattgcatc agtaaaaata atttccctta ctttttcttt gtttattctt ccatcttttt
     5821 caatatcttc tccaaaaatt ttctttattt tttttaaaat attgtttctt ttccataaat
     5881 tatgtacaat ttcatcacat gaaataactt taaatttcca ttttctaaga atttctgtta
     5941 tagtagtttt tcctgaacca aggattccag taattccaac aataatggat tttttcataa
     6001 agttcctaat tcccaggaag agaggtatct ttcttgtaaa tctgttaaac tgtcaatttt
     6061 tatattcatt gtattaagtt tatattttgc aatttgttta tctatttctt caggtacatc
     6121 gtaaacattt actttaagat tgggatgttc ttttataaat ttaatagata aaaattgatt
     6181 tgcaaaactc atatccataa cttccggtgg atgtccttct gcacatgcaa gattaagtaa
     6241 tctaccttct cccagtataa aaattttttt accattttta aggatatatt cagtaagtcc
     6301 atttcttatt ctttttttcc ttaaagtatt ttcttctatt tcatccattt ttatttcaac
     6361 attaaaatga ccagaatttc caagaataac accatctttc attttattta tttctttcat
     6421 tgttattata gatgtatttc cagttgcagt aataaatata tctccaattt tacttgcctc
     6481 tttcattgac ataactaaaa atccatccat tactgcttca agggctttta ttgaatcaac
     6541 ttctactact attacccttc ctcccatccc tctgaatctc cgggcaattc ctttaccgca
     6601 ccagccatat ccacaaacaa ctattatttt acccgcaatt aaaatatttg ttgctcttaa
     6661 caatccgtct acggttgatt gtcctgttcc atatctgtta tcaaaaaggt atttcgtttt
     6721 tgcattattg actgcaatta caggaaattt taaaagtttc tgtttattta agtttttaag
     6781 gcgaataaca cctgtagttg tttcttctgt tgctccaatg atatttttta tatatttttc
     6841 attttgatga acaaaagtaa tcaaatcgcc tccgtcatct attataaaat ggggcatatt
     6901 ttttgctaca ttttctatat ttttataata ttcagaacca ctttcgcctt tttttccaaa
     6961 aacaggaata ccctttttaa caagatatgc cgctacttcg tcctgagtac ttaaaggatt
     7021 tgatgcacac agaaaaactt ctgcaccaca ctctttaaag tttataatta agttcgcagt
     7081 ttcagaagtt atatgcaaac aggcacctat tttataacct ttaaaaattt ttcttcttga
     7141 atatttattc cttaaatact ttataacggg catatgctgt aaagcccaat ttactttatt
     7201 ttcaccttct ctataaaggt ttatatcttt tatagaatat tccattttaa aattatctga
     7261 tattttttgt aaatatttct tttgaatcag ttttttccca tgtaaaatcc tcatcatttc
     7321 ttccaaaatg tccataacag gcagttttcc taaaaattgg tcttaaaagg tcaagttttt
     7381 ttataattcc tctcggagaa aggtcaaaat tatcttttat aatttttaca atttcactat
     7441 caggaattaa tcctgttcca taagtattta tcattaaaga taccggttct acctgtccaa
     7501 taacataagc aatttgtatt tcacattttc tggctatacc agaagccaca atatttttag
     7561 cgagatatct tgccatataa gaacctgatc tgtctacttt tgttgggtcc ttaccagaaa
     7621 aacatccacc tccatgactt ccaactccac cataagtatc tacaataatt tttctacccg
     7681 taacaccggt atctgccatt ggaccaccaa taataaaact tccagtggga ttcgtaaaaa
     7741 ttttcatttt tttacttctt aattcttctg gaattacata atttattaca agttctatta
     7801 tgtcatcttt aatctttttc tgtgatacac caggattatg ttgtgctgat acaacaacac
     7861 tttcaacgga tacaggtttt tcattttcat aaagaactgt tacctgtgat tttccatcag
     7921 gtcttaaata atcaactatt tttttctttc ttatttctgc taatctcatt acaagtttat
     7981 gggcaagtgt tattggcaaa ggcataagtt caggtgtttc atctgttgcg tatccaaaca
     8041 tcattccctg gtctcctgca cctcctttat caactcctgt ggctatttca ggtgcttgtt
     8101 cttgaataga tgttattata ccgacagatt catactgaaa tccgtaggat ggatcaatat
     8161 aaccaatttc ttttaataaa tttcttgcaa gttcaggtat atcaacataa ccagttgttg
     8221 taatttcacc tgcaataaat aaaagccctc ttgttgttaa tgtttcacag gcaactcttc
     8281 cgtttttatc ctgtttcaaa acttcgtcaa gaatcgaatc tgaaatctga tcgcatattt
     8341 tatcaggatg tccttcacaa acagattcag atgtaaatag ataataattt ttcacttttt
     8401 tcctcctttt ttcatttctt cttttaaaat atttatctct tttatttctg tggggtcaac
     8461 tttatttaag attgcaaggt aataacttat ccagtcacca agtattatca aatacattgt
     8521 tttttcaaga agtgtttctc cttccagttt taattccata acctccttta ctttttttga
     8581 aataatattt gaagttattt caattctttt tttaactctt tcattttcaa taccagaaac
     8641 tataaacaga caaatcgctt tttttataaa gaattcagga aaatagaaag ccattatttc
     8701 attatgattc atttcaggta aaatattaac aaaagcgaaa ttttttgaat tttctgctat
     8761 ttgtgttttc catcttaaaa ttcctggata aaatctattt tcagaataaa tcagtggaat
     8821 atttttataa attttttttg aaatttctat ggctttatta ttttcacttg agggtaaaaa
     8881 ttcatttacc catttttcaa ttttttcaaa gaaagttttt tcaagaggtg gaagtatttt
     8941 tgcatcaaca aaatatctat atacaggaaa aaaaagatat ccaaaagcac atcttggagg
     9001 catccctgaa ggtattttaa ttaaaggaat tccgtcagaa catgccattt tttcaatttc
     9061 tccattactt gaaatacaaa ttattaaact cttatttcct tttgcaattt tatatgtatt
     9121 tactgtctcc tctgtattgc ctgaataact ttccacaata acaagcgttt ttgaatctac
     9181 atattttggt agtgaataat cccttataac aaaaactgga atttcagcaa tttcttctat
     9241 tagaattttt atgatatctc cacttattgc agaaccaccc attcccgtaa agacaacttt
     9301 ttcaaaattt ttatttattt ctgaaatagt taaattatat ccttccagac aatgtcttgg
     9361 aaaattcttt aataaagaaa acatgtcact tttatcatat ttttccataa gtgatttttt
     9421 taattccatt ttttatcccc ttttatttta aattttttct taaaaaattt attatctctt
     9481 catgtttatt aaaattcata actttttctg ctatttcttt catttcagtg taactatttt
     9541 ttataatttt ttctttgaca gatgggattg ctactggtgc catacttaac ttctttatcc
     9601 ctaaaccaat taatagacat gctatttccg gaatagaagc catttctcca cataaactga
     9661 cctcaatatt attttttata gcattttctg cagtaatttt aattaatttt aaaactgatg
     9721 gatgacatgg ttgatataaa taagatagtt tctcatttac tctatctatt gcgagggtat
     9781 attgaattaa gtcgtttgaa ccaatactga agaaattaac ctcttttgcc aggtcatctg
     9841 aaactaatgc tgcagaaggt gtttctatca taattcctac ctgaatattt tctttaaact
     9901 ttagattttc ttttcttagt tctttttttg cacattctat tatttttttt gcttctatta
     9961 cctcttcttt tgttgtaatc attggaaaca tcacttttaa gtttccataa taacctgctc
    10021 ttaaaatcgc tttgatttga gttaaaaaaa catctctttt ttcaagacaa aatcttatac
    10081 ctcttaaacc aagaaaagaa tttaactctt ttggtccctg aaaagcggaa ataaatttat
    10141 caccaccaac atctattgtc cttataataa tttctttgtt accaaatttt tcaattattt
    10201 ttttatatga aagaaattgt tcttcttccg ttggtaaatc ttttcttttt aagtaaagat
    10261 actctgttct gaataaacca atcccttctg ctccatactt taaagcagtt tcaacttcct
    10321 caggtagttg aatatttgca tataaattta tttcttttcc atcagaagtt atggtttttt
    10381 tacctttcaa aatatatatt tttttcctga attttctaat ttccttttct ttacttttta
    10441 ccttttcaat ttctatttga gtgggattga ctattacagt accttcataa ccatcaatta
    10501 aaattgtgtc accatctttt atctcctcaa tattttcttt tatcccaaca actgcaggaa
    10561 tttcaagggc ttttgctact atagcggtat gagatgtttc tgttcccata gaaatcacaa
    10621 agccctttat atatttttta tcaattgaaa cagtctgtgt tggagaaaga tcatttgcca
    10681 caagaattcc atcttttata tcttttaatc tgtcattttt tttaattaaa tttgcaatta
    10741 ttctttcaaa aatatccagt atttcttttt tccttgtttt aagattattt tcagaaaatt
    10801 ccttattcaa attaacaaca aatttcttta cggctgtttc agcagaaacc ctttctcttt
    10861 ttataatctt ttctattcgc tccattaaat ttttatctct aaggatagaa agatgaaaag
    10921 aaaaaatttc tgcatattcc ttatctgtta ttttctttat gttgtttata atttgctgaa
    10981 tttcattttc tgtcttggta atactttcat gaaatctttt aatttcttca tctacatctt
    11041 cttcttttat tttttttatt tcactaaaag aaaaaaaatc atcattcacc tttcttgctt
    11101 ttccaatagc aaaccctggg gaagctggaa ttccttttat ttttttcatt ttttaatttt
    11161 tttaagatag actattcaat ttttcttcat cttcttttat gattttaatt gcttcttctg
    11221 gtgttttaca gtttcttaaa gcattcctga aaactttgtc tttcagtaat cttgctattt
    11281 ttgcaagagt ttttaaatgt tgacctatag attttgtcgg tgcaagaaca agaaatacta
    11341 tataaacagg ttctccgtca agagaatcaa aatccactcc tgtttgtgaa agtccaagtg
    11401 cacatataat atcatttatc tggtctgttt ttgcatgagg aattgcaatt ccctgtccaa
    11461 tacctgttga accaagtttt tctctatcca taactgtttc caatattttt tcgctatctt
    11521 tcacatatcc atttttttca agtgttttta ttatttctct taatactccc tttttatctc
    11581 tacttttgag attagtaata atacaatctt cttttaaata atcaactatt ttcatttttt
    11641 ttcctccttt tttatttgtt tacaatttta tcatgtaatt ttcttaactt tacttttgct
    11701 ttgtccagta aattttcaac acttttattt acatcgttat ctttttcttt tagaaaaact
    11761 tttatatttt ttgaatgtaa ataaatctct gttaaaaata aaggcccatc tttttttatg
    11821 taaaattcag catatccatc atcaaataca aattttttta acttttcaat tttttccttt
    11881 atgtaatctt caaattcttt atttatttct acttctgaat aaatttttat atccattttt
    11941 tctccttctc atttaataaa ttttttttat ttttaaccca ggaaaataaa attttaatat
    12001 gtctttatag tcaaatccaa tttctgccat tttttttgca ccatattgag aaagccctac
    12061 accatgtcca taacctttac caataaaagt tatagaatct cctttgtccc gaacttcaaa
    12121 cagtgtactg ggaattatat aaggattatt aaaataaacc tctgtttttt gattaatttt
    12181 ttctctaaat tctgttcctc ttaattttat attaccattt tttgttgtaa tttgcaaaag
    12241 ttttactcta ttagtttggt ctctttcaat tacttcaatt ttttctattg aacatcctaa
    12301 aatttttgaa aggtaatttt ttgaaatatt tttttcccag taagaaaaag gattatcttc
    12361 tgaatacgga tctataactg aataatttaa aggatatttc cccttccaga catcagaagg
    12421 atttgcagtt ataccacctg aacatgcgtg aaaaaaaaca ggaactattt tttcattttc
    12481 tgtcaataaa acttctcctt ttgtttcaag taatatggat tttatttttt catctttaca
    12541 atctttatat aattggtggt ttattgaatt ttcaatatga taataatttc ttttgtttgt
    12601 acttattttc catattaaat aagttcttga aacaactgcc tgtgccttta atgcctcaac
    12661 tggccaattt tctcccattt cttttacaac tacaccagaa atatattcct ctatttcaat
    12721 ttctttaaca gtatttcctt caagtaattt tataaatatt gtttcttctt cagcaaaaat
    12781 aaaataattt atacacatga aaaaaaggaa aacattattt agaaactttt tcttcaggta
    12841 caatttcaac atgtacattc ccttttattt ctattgtatt attatcaata ttgtaaatta
    12901 ctttttctcc cgaaaattta ttttttcctc tttttattaa cggtttttct tcaaaaatca
    12961 tttctctttt ttcatcaata taactaactt ttccacaggt gatttccatt gttacatttt
    13021 cagtagtttt atccttatat aaaataatca cttttcctgt tatcataatc tctttaagtt
    13081 ttttattttc cggatcaatt tttatttcac ctttaccttc tttatcagtt ttaatctcta
    13141 taatttcttt atctgttttt aatgataaat taggtgcaga aattttagta tttttatttt
    13201 ctgttatttc ggcactttct ccaatcaaag taatcgaata attttttgaa aagtgtttta
    13261 atgaaaattc agcaatcttt tcatctttag aaataatttc ttcgtttttc ttttttgtac
    13321 aggaaacaaa aatgaaaaac ataaaggaaa ataaaattag atttttaatt atttttttca
    13381 ttttattacc ccggtggcca catcattatt cttccgccta taagatgaac atgaatatgg
    13441 tctacactct gtcctgcatt tctattagta ttaattacta tcctaaaacc agaatccttt
    13501 atcccttctt tttctgcgat ttgacaggca atttttagca ttttccccag gagaaatgta
    13561 tcttctactt cgtaaagttt ttctatatgt tttttgggaa ttataagtat atgaacgggt
    13621 gcctgtggat ttatatctct tatacaaacc atatcatcat cttcataaat tttatcactt
    13681 tttatctcat tatttataat tttacaaaat acacaattca tattttaccc tccttttaat
    13741 tttctgggca cctcaatagt aataaaagat tgaattatat ctccttcctg aaattcatag
    13801 aaattgtcaa ttccaatacc acattcatta ttataattta cttcatttac ggtatcctta
    13861 aatcttttta aacttgttaa atttccttca aaaataactt ttccgtttct tactatctta
    13921 atttttgaat tttttactac tttaccactt actactacac aaccagcaac tatattgttt
    13981 tttgcaagtt taaaaacttt ttttacaagt gcctgaccaa ccatttcttc ttttatttcc
    14041 ggttcaagca ttccctctat tgcttttata atatcatctc caatatcata tattaattca
    14101 taagttctaa cttcaatatt ttcttttttt gcaagttctt ttgcttttgg ttctacagga
    14161 accttaaatc caagaacaat accattggaa gcagaagcaa gtaaaacatc tgattcactt
    14221 ataggtccag ttccagcatg aattatatta atttttattt cattctgtgg aattttgtta
    14281 aaaacatttt taattgcatc tattgaatta taaaagtcag tttttaaaat aacatttaat
    14341 tctttcacct ctcctttttt tatttcttca taaaggtctt ttaatgtcaa tctttttacc
    14401 gggattacaa tactttcttt ttcccttttt aatttctctg aaaaatcctt tgcttctttt
    14461 tccgaatcaa ctactctaaa tttttctccc ggaattgcta tttcatgagc accaagtatt
    14521 tcaacaggag tagatggacc tgcttcattt aatcttttac cccactcatc aataattgct
    14581 cttacttttc cccaggtact accaacaata aaagggtctc caacttttaa ttttccattt
    14641 tcaataagta cacttattac aggacctctg aatttatcaa ctgaagattc aatgacagtt
    14701 ccattacatt tgcagtttat aattgctttt aattcaagca tttctccaac aagaagtatc
    14761 atttcaagta aatcatttat tccttgtcct gttaatccag aaacatttac ataaactgta
    14821 tttcctcccc attcttctgg aattaatcca tattcagaaa gttgctgttt aactttttct
    14881 ggatttgcat ttggtctgtc aattttattt atggcaacta ttataggtac attagcagat
    14941 ttagcgtggt ctaaagcttc aattgtctgt ggttttactc cttcatcagc agcaacaaca
    15001 agaactacta tatctgtaat tttagctccc attgctctca tcgctgtaaa tgcttcatgt
    15061 ccaggagtat caagaaagac aattgaacct tcgggtaaat taactttata tgcacctatt
    15121 ttttgagtaa tctgaccgaa ttctttttcc gcaaccttac tgtttcttat tttatcaagt
    15181 attgttgttt ttccatggtc tacatgtccc ataacagtaa ctacaggtgc tcttttaaca
    15241 tatattggtt tctcttcttt ttctgttttt tctaaaattt taggttgttg ttcttcttta
    15301 aatacaggaa taaagttgaa attttcacag attttttgga ttatatcttt tctaagagtt
    15361 tgattgatat tgacaattat tttttgttca agcaggaact tttgtatttc agtagattgt
    15421 atctggattt tttctgataa ctcttttata ttctctttcc ccgttaaaat aatttctttt
    15481 tttattactt cttcttttaa tttttcttct ttttcgggtt tccctttttc ttctattatc
    15541 tccttttttc cttctgactt ttctcctttt tctttatctt tttcaaaata gtcatcaatt
    15601 ttttttattt cctcatctgt aataacactt aatgtttgtt ttcctgtaat gccaatattt
    15661 tctaatactt tcagcaaatc ccttgtttgc aaattatact ttttggccaa ctcgtagatt
    15721 ctcatttttt taaatatttg tttccttttt gtattcagat actttaaaaa ccttaatatt
    15781 ccaaccggtt aatttagagg caaggcgaac attctgacct tttttcccaa ttgcaagaaa
    15841 aagttggtca tcatcaacaa ttgccactgc ttccttcttt tcttcatcta tatggacttc
    15901 attgcatttt gcaggagaaa gtgaattttt tatatacttt gcaatatctt catcccataa
    15961 tattaattct attttttcac ctcttaattc tttaactata ttttttattc ttgaagcttt
    16021 atcccctata catgtcccaa cagggtcaat tttttcattt ttagagaaaa cagcaacctt
    16081 tgttaaatca ccaggaaatc ttgcaattgc ttttatttca actattccct cttttatttc
    16141 aggaatttca ttttcaagta attttttaac aaaatttggg tgagttcttg aaaatattaa
    16201 ctgataatta ccttttaaag gaggtctaac ttcgataaga taacatttta taccatcccc
    16261 taatttaaaa tggtcattgg gaagtttatg atgatgtggt aaaactcctt ctgcttttcc
    16321 tgtaacaact ataacattat cattttcaaa tctttcaact ctcccactta taatattccc
    16381 ttccagattt ttaaattcat tgtaaattgt acttttttct gcttctttta ttttctgtat
    16441 cagaacatgt cttgcagttt gagcagcaat tctttcccac ggaaatgaag gaggaggaac
    16501 ttcttcccca ttctcattta taaattttat atctcctgtt tcaggattga ttttaatttt
    16561 tatatgcttt ggaagttgtg cttttttcct gtaaacagtc aaaagaccat cctccaatga
    16621 ttctattaga tggtttcttt ctattcctct ttccttttcc caatattcca ataaagaaat
    16681 aatttctttt ctcatgtttt ccacctctat tttaatttta tttgatttga tttgatatgt
    16741 caagtaatgt taaactaaaa catatgaaaa ataaatcaat ttttctcatt ttactttttg
    16801 gaataataag tttatttgga gatattgttt acgaaggagt aagaagtata agtggaccat
    16861 ttcttgcttc tattggtgct tctgccgtta ttataggatt tttttcaggt ttaggtgagt
    16921 ttttaacata ttttttaaga tttttttctg gttatatttc tgacaaaaaa aggatatact
    16981 ggccatttac aataatagga tactctttaa tatgctgttt acctctactt gcttttacta
    17041 aaatctggca aattgcagtt atattacttt tattggaaag aataggaaaa gcaataagaa
    17101 ctccttcaag agatacttta atttcttttg ccggaaagga tattgggtat ggaaaaagtt
    17161 ttggaattca cgaagtttta gaccagattg gcgcaatttt aggacctcta atttttttct
    17221 tttccctttc aagatttaaa agttattcag ttggttttaa attcatgtgg gtgccatctt
    17281 tttttctaat cctaacactt atttttgcaa ggaaaaatta taatattatt gttgaaaaag
    17341 aagaaaaaat taacttttta caaaaaaata atctaaatca gaattttact ttttatcttc
    17401 tttttgtatt tttttcttta tcaggtctta taacttttcc gattatatca tatcatctga
    17461 gtactaaaaa aattgtaatt ttttccggta tcccggtttt ttatattatt gcaatgagtg
    17521 ttgatggaat agttgcttta tttattggta atttatatga taaaattaaa cttaaaacat
    17581 tgcttttaat tccttttcta actatagttt tatctttttc tgtgctttct acaaatatct
    17641 ttttttcttt aataggtata ataatttttg gtgtaattat aggatgtcat gaaacaattt
    17701 taagagcatc aatagcagat atgattcctt cttttcaaag gggttttgct tatggtattt
    17761 ttaataccat ttatggaatt gctcttttta tcggaagttg gataatcggt tttttgtatg
    17821 gaaagaattt taaactcata tgggtttatg taattataat ggaaattatt tcttttgttt
    17881 ttctgtttta tcagataaaa ataaaaaatg ttgtggaaaa agcacggtaa atttgaattg
    17941 ggaaaaaata aagttgagat aagaagtata aaattaaaag acgaaatagg taagataaga
    18001 agattaagga tttgttcagt atggagtaat ttttcaaaat ttactaaaat tcctggggaa
    18061 ggttgtttat gtaaagatga aaagggttac attggtgtgc ttttaactgg aaaaaatggt
    18121 gcttatataa aagtcggtaa aaaattttta atatcacaga atttaattgt tccatttaat
    18181 tcaataagta agacatcttt aaaaaaactt ttaaaaggtt ataatattga tttttttgaa
    18241 aatggggatg caatttatgg attagaaaaa tgaaaaaaaa acagattatt acacccgaag
    18301 aaataataaa taaaatgagg gatttttgct ttgaaaagaa aataaacatt tttcttgttg
    18361 gaggatattt aagagataaa tttttaaaaa agaaaagcaa agatattgat tttgtgatgg
    18421 aagaagatgc tttaaaaatt gcagaagagt tttcaaaaag attcaattta cctgcaccag
    18481 ttttttatgg aaggtttgga acagcaatga ttgaaataga aaatattaaa cttgaatttg
    18541 caacagcaag gaaagaaagt tattttgaaa attcaagaaa accagttgtt gagaaatgtt
    18601 ccatttttga agaccttgca agaagagatt ttactataaa tgctatagca caaaatttaa
    18661 ttactggaga aattcttgac ccttttgagg gaagaaaaga tttaaaaaat aaaattataa
    18721 gaaccccagt agaaccagat aaaacatttt ttgacgaccc tttaagaatt ttaagaggga
    18781 taagattcgc tacaaaattt aaatttaaaa ttgatgaaaa aacattagaa gggatgaaaa
    18841 aaaacaaaga taggataaaa atcgtcagtg aggagagaat tgctgatgag ataatgaaaa
    18901 ttcttgaaag ttctactcct tctattggat ttaaattaat ggatgaaatt ggacttcttg
    18961 aaattattct tccagaagta gcaaatttaa aagaaaaaag gactgaccat ccatgtaaag
    19021 aactttttcc ccatactcta aaaactcttg acaatatttc taaatacaca aaaaatgtat
    19081 atttgcgact tgctgctttg cttcacgata ttggaaaacc ctatacattg gcagttgaag
    19141 atggaaaagt tagttttcac aggcatgaat ttgtaggaga aaaaatggct tataatgttt
    19201 gtagaaggtt aaaaattggt gaaccagaaa taagatttat tggaaggtta ataaaatttc
    19261 atttaagacc tcatttactt gcaaaagaaa atccatctga cagtgcttta agaagattta
    19321 taaaagaaat tggaaaggat atcaaacctc tttttattct tgcaaaaagt gatttaacaa
    19381 gtagaaatcc tttgaaaata caggaagcac tggaacgtct tgaacgattg gaaacaagaa
    19441 ttaaagaaat taataagaag gataaacttt cttcttttaa acttgctata aatggttttg
    19501 atataatgga aattttaaaa attacaccgg gcaaaaaagt cggaataatt aaagaatatc
    19561 ttgaaaatct tgtaatagag ggacagttgc caaataaaaa aagaattttg cgcaggtatt
    19621 taattgaaaa cgtagaaaaa cttttgaatt taaacgaata aaatgaggat aggtattgat
    19681 atttcatcta tattatcaaa gaggaagaca gggataggct ggtatacata ttatcttata
    19741 aaaaatctta tagaggtaga taaagagaat gaatatgtat tatttggttg cagtataggt
    19801 aattatagag ggaaatttaa tttaaaaggt gattttaatg gagagaattt gaatatttct
    19861 gtgaaatttt tgccgaataa attaatgtca tctcttcata tatttttccc tatagagttt
    19921 ttacatggaa gatttgatgt aatacatata acacttcatc cttattctgt tttaaatata
    19981 tttggaaaac agatagttac tgttcatgat ttatgtcatc ttatcaattc aaaatggttt
    20041 catccagtag aaagttatcc atttaagatt gttattacca aaacagttca aagggtggat
    20101 agaataatag cggtttcaga atctacaaaa agggatttaa tagagtattt aggagtgaag
    20161 tcagagaaag tagaggttat ttatgaaggg gtagatgaga ttttcagagt agtagaggat
    20221 agggagaaga tagaggaagt aaagaggaga tatggaataa gtaagaggta tattttaaca
    20281 gtagggacga tagagccaaa gaagaatcat atcaggttat taaatgtttt caaaaggata
    20341 agggagaaaa gagaagatta tcaacttgtc atatgcggga aatacggatg gaagacagag
    20401 agatttttag aaagattaag tggtttaccg gattttataa gaaaggatat aataattacc
    20461 gaatatgtta gatatgaaga tttaccttta ctttataatg gttgtgatat atttatttat
    20521 ccgtcattat atgaag
//
LOCUS       DAOSAT010000019        19819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_1931_length_19819_cov_5.054645, whole genome shotgun sequence.
ACCESSION   DAOSAT010000019 DAOSAT010000000
VERSION     DAOSAT010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19819
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1931_length_19819_cov_5.054645"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PLF90_02810"
     CDS             <1..760
                     /locus_tag="PLF90_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56249.1"
                     /translation="ANSAGFNDYKEFLKHILTEIEKHAIEKNWLPVIFALYDEPVSKE
                     AKEATISYCLIWKDITKNLTKIKTTGYTSMSSSGEIDETQIKLATSVDIVSLNIHDEK
                     ALDVLREKGTGWAYYNNGNRFTYGIYLFKATKELNLHHRIAWHWNVCAGDPYYALDCR
                     EDDYAWFVTNAKKELIPSIFFLRLAEGIDDYRYLLTLSRLINEKPNHTYSKEAKRIID
                     ETLSSIKVGQRTYPPTAYREIRYKVAEAIEKLLK"
     gene            761..2188
                     /locus_tag="PLF90_02815"
     CDS             761..2188
                     /locus_tag="PLF90_02815"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HOK56250.1"
                     /translation="MKKERVYFTKISGSYSPNEIYKKVRPLESKEFILNPHKGFATFQ
                     HFNGDPLFGEGGDRDDLPMDAAGPLEFELTKKEPEVVDGYLPTTIAYCRWFWKIFEPE
                     EGKFNWDMVEKSLKVAELRGQSLQVRLMPFGSKNQPQLPEWYIKIAPTRKCKFYEGVL
                     EPDYDEQTYFKYWSRVIEEFTKKFANHPNLESFDIAFIGPWGEGAGEIKDETVEKFID
                     MYLRFFDKKKLLVNIEGYQFIYGIKKGVGWRCDCFGDMRNAGVNVVPDGLGWNHMFDV
                     YPKYVCRASAQEVWKNVPVVFETCHTPLWWYENDMPLEFLDFIIEQGYKYHCSVFMPK
                     YTRIPNAYKEKFLKFADKIGYKFVIRQLKFERKLKGKRKFKYEMWIENVGVAPLYKNK
                     YKLAFKFKQGKKEEIVQSSQDITKWLPGDIWIEEELELNTNFEEGKIDIYVAIVDSET
                     NKPKIKFAIEGKKENGWYFMEQVEK"
     gene            complement(2192..2935)
                     /locus_tag="PLF90_02820"
     CDS             complement(2192..2935)
                     /locus_tag="PLF90_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOK56251.1"
                     /translation="MESIYNLSHFKLFLLELYAFLIGVSKTGIPGVGILAIPLTAMIL
                     PAKASTGLILPMLIMGDIFAVTYYRRKAVWKYILRLLPFAATGIIIGYFLMGKINDKQ
                     LSKFIGLVILTILFLNWWWNKKEIKVPEKFYFAVIMGLFAGITTMMANAAGPILIIYL
                     LAMKLPKTEFVGTGAWYFFILNWFKVPFSVNLGLINFYSLKLNLLLFPCVCIGAISGI
                     FFLKKIPQKVFNQIVQILAFLSALKLIFF"
     gene            3070..4869
                     /locus_tag="PLF90_02825"
     CDS             3070..4869
                     /locus_tag="PLF90_02825"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOK56252.1"
                     /translation="MAENFDWMNNLKKERPRLILNDKVVEDIKTLIKNDDTCKKIYVK
                     IKENAEKICDEPVSKYEIPDGLRLLYVSIKVLNRVYTLSLVYKIEGNKKYLERLWNEI
                     YTAGNFPDWNPKHFLDTAEMAHAFAIAYDWCYYDWTEDQRKIIREALLNKALKVALDC
                     YEGKIPYGWWTRSKHNWNQVCNGGIGIACLSLLDEYPEICSKILDNIMKSLPLAMKEF
                     APDGGWGEGPGYWAYATEYNFIFLTYLDNCINYNFDFWNYEGVSETGFFPIYITGPVD
                     KTFNFADSGDGPVRSSQLFYLSKKFKKPVFAWYPIKYPRYHPLDLIWFTDYSDSEKEN
                     LPLDKYFRYVEVVTMRTSWNNRDALFVGFKGGRNNFNHSHLDIGSFVFDAYGYRWIID
                     LGLDNYNLPGYFGKNRWDFYRLRAEGHNTIVINPSEKFDQEPDAISKIVNFGSKNEYS
                     FAISDLTDAYRKYAKSVKRGILLNKKEKFLLIQDEIELKEKGEIWWFLHTGCDIEIGE
                     SKKEVILKQKNTELKVKVISPSDAQFLIMDAKPLSSSPNPTGQNENKGIKKIALNFKN
                     TDNLRISVILVPLKPGNKFSPFTPSLIPLNDWQ"
     gene            4885..5757
                     /locus_tag="PLF90_02830"
     CDS             4885..5757
                     /locus_tag="PLF90_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOK56253.1"
                     /translation="MEKIIHIIWTMDCEFPKSPFGGPENWEIAEKSVNGFLEVLTKHC
                     QKATFFVTPEVIQKKFNVFSEKNKNFEIGMHLHPFDDSNKKKLYMGQMRKEEQEKVLK
                     ENREKWIQSAGFEPRSFRPGNFSANDYTFPLLLKLGFFQGSVSSPYRKMEKFFTDWEG
                     ALLDPHHTNEKSRLIEGKLDFFEVPVTVDPFRKKRIDGTSYELRIEWGDISEHIVTIE
                     NVLKRLKKENPPVKSIVSITHNTFDYTLKNSKYRSTLEKLILFIQELIIKENFTPLSI
                     TIEELHKKVDGKDL"
     gene            5831..6853
                     /locus_tag="PLF90_02835"
     CDS             5831..6853
                     /locus_tag="PLF90_02835"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOK56254.1"
                     /translation="MKKSKKNYLKIPVEHVDIKKIDAIPIVEMYSRMSFSARTLAYGA
                     LLYEKMLKDEEVTVILTLAGSLFSAGLKNAVCDLVRNNLVDIIVSTGAIIVDQDFFEA
                     LGFRHYIGSPDVDDRELNKLHIDRIYDTYIDEDELRICDMTVAEIAEKLEPKPYSSRE
                     FIKEMGRYLKEKGIKSDDSVVLACYEKNVPIFVPAFSDCSAGFGLVYHQIKHPEKHVS
                     IDSVKDFKELTELKIAANQTGVVMLGGGVPKNFTQDIVVCADVLNKTVPMHKYAIQIT
                     VADVRDGALSSSTLKEAHSWGKVDFPNTEMIYSEVTVAFPIIAAYVYQKVKNRKPRNW
                     NKFFEK"
     gene            6935..7933
                     /locus_tag="PLF90_02840"
     CDS             6935..7933
                     /locus_tag="PLF90_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOK56255.1"
                     /translation="MDKQIPSLCEVYKDYFPIGAAVNLKTVVSHADLLKKHFNSITPE
                     NHTKWGLIHPLPNKYNFEDADKIINFAIENKMKIRGHTLVWHIQVPEWVFKDEKGNWI
                     DKDTLLKRLYEHIKTVVGYYKGKVYAWDVVNEAISDKEGEFLRDEPWYKIGGEEVIEK
                     AFIWAHEVDPDAILFYNDYNLELPSKREKAYQLIKKLKEKGIPVGGIGIQAHWGLYGI
                     TPEDLEESIKKFSTLGVQIQITEFDISIYLDKNEKFNSIEEVPEERIKKQIELYKKVF
                     EILRKHKNLITGVTFWGVADDITWLDYFPVKGRKNFPLLFDIYHKPKKVFWEIINF"
     gene            complement(7944..8552)
                     /locus_tag="PLF90_02845"
     CDS             complement(7944..8552)
                     /locus_tag="PLF90_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56256.1"
                     /translation="MKFLLQFFLSGFLVGISFCGFHCCFLLFPVVIKESDNWKGGIKI
                     ASLFSISKIFIYGFIGGLASYFGNYIQSIIQKEVFSYISGLILILIGILFLFPSKKYV
                     KVFKTSTPIFLGIIEGITPCAPLIGLIFYIAYLNKGIFFGFSAGFLFALSSIIFPVLL
                     IIGFIPYFLQKILFHSKVKVLFKFLGFLIFIYWGINLILHKF"
     gene            8626..9198
                     /locus_tag="PLF90_02850"
     CDS             8626..9198
                     /locus_tag="PLF90_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOK56257.1"
                     /translation="MIYYKKKEIKMTRDDGFELLKKYISNKNLIKHSLACEAIMRKLA
                     KYFGENEEIWGLAGLLHDLDYEYTKDNPEIHGLKTIEMLGDSVNEEIKNAILAHAEKK
                     NPETKIEKSLYAVDPVSGFIVACVLIRPEKKLEIIDIEFLKNRFKEKSFAKGANREQI
                     KTCETLGIKLDEFLSLSLESMKKIAVELGL"
     gene            9243..9314
                     /locus_tag="PLF90_02855"
     tRNA            9243..9314
                     /locus_tag="PLF90_02855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9275..9277,aa:Thr,seq:tgt)
     gene            9334..9870
                     /gene="dcd"
                     /locus_tag="PLF90_02860"
     CDS             9334..9870
                     /gene="dcd"
                     /locus_tag="PLF90_02860"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOK56258.1"
                     /translation="MVKSDKWIKKMAQKGMIEPFFPEKIKKGISFGLSSYGYDFTISN
                     EFLIFKGKIASPKQITENDFEKYKGNVCKIPPNSFVLGKSVEYFRIPREVIGICFGKS
                     TYARCGIIINVTPLEPEWEGFITIQIANLTPVEGEVYAGEGIGQVIFLSADEICEKSY
                     KDHSGKYNNAKDIQLPKI"
     gene            10261..10641
                     /locus_tag="PLF90_02865"
                     /pseudo
     CDS             10261..10641
                     /locus_tag="PLF90_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
     gene            10765..11190
                     /locus_tag="PLF90_02870"
     CDS             10765..11190
                     /locus_tag="PLF90_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOK56259.1"
                     /translation="MKNLTKKTYKNPIPTVDIIIEYNDGIVLIERKNYPFGWAIPGGF
                     VEYGESCEETAIREAKEETGLELEDLRQFKTYSKPGRDPRFHTITTVFTAKGKGYLKS
                     GDDAKNASVFNEKNLPENIAFDHRDVLMEYFYFKNKLRN"
     gene            11225..12217
                     /gene="trpS"
                     /locus_tag="PLF90_02875"
     CDS             11225..12217
                     /gene="trpS"
                     /locus_tag="PLF90_02875"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOK56260.1"
                     /translation="MKKIILSGMRPTGPLHIGHLFGALKNWKKLQDEGYKCFYMIADY
                     HALSTEYANPRKIKEYVEEMAIDFISSGLDPEKSTIFIQSLVPEHTELHLIFSMIVPI
                     PYLERNPVYKEQIEELKDKELHTYGFLGYPVLQAADILIYKANFVPIGIDQLPHLELT
                     REIARKFNYLYGETFPEPEPLLTETPKIPGTDGRKMSKSYNNCIFLKDSPEIIEEKVS
                     KMFTDPKRIYRRDPGHPETCPVFAYHKIFNEKRVGEIEKDCKGAVIGCTDCKKELGKK
                     IVEYLEEIRKKREKIEKNKKDLMEILLEGSKKARETAIKTIYEVKEKIGVKYEK"
     gene            12207..13307
                     /gene="hisC"
                     /locus_tag="PLF90_02880"
     CDS             12207..13307
                     /gene="hisC"
                     /locus_tag="PLF90_02880"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOK56261.1"
                     /translation="MKNNIEKLVRKELKEIAPYVPGKPIDEVKRIYNIDRIIKLASNE
                     NPLGYSPKVKEVIKENLDDINRYPDGSGYYLKKELANFLNISEDEIILGSGSSEVISL
                     AIEAFVNPGEEVIYPFPSFIIYRILVLKVGGVPVEVHLENDFSYNIDRILEKINSKTK
                     VIILCNPNNPTGTILYKNQLERFLNKIPDNIIVISDEAYFEYVEDKNFGTCFPYYKEK
                     NIVITRSFSKIYGLASLRIGYGIGKSEILNYMERIRPPFNTTGIAQIAAITALKDMDF
                     VKKSIENNIKGKKFLYENFKKLGIDYIPTEANFILCRFKGDATFIVKELEKAGVIVRG
                     MKSFGLSNEYVRITIGTEEENRILIEKLSKLI"
     gene            complement(13313..14245)
                     /locus_tag="PLF90_02885"
     CDS             complement(13313..14245)
                     /locus_tag="PLF90_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOK56262.1"
                     /translation="MRGYSVFYMDKEKFEIREHEIEDVKNDEVMIKCLANGICMWESS
                     LFAGIEKLPENSIIGHEGIGVVVEKGKEVKNIKEGDYVVCFNWSLYQKMKEKDVKKFS
                     IKVDDPAKYIVEPVACVLNAINFYSVSPGDKIALIGAGFMGLLNLQGLSGFPVEEIVV
                     FDIKEYNLKLAEEYGATETVNIKKGFDIEKYRNRFDIVIECAGVQEALNLAEEIINKA
                     GKLAIFSWHHGERTVNAGLWHSKGLHILNPSPVMGIKRNMDNFERGIKLLENRKFNLE
                     KLITHRFSVKEIDKAMRTTLEKPEGYIKAVLLFE"
     gene            14491..15270
                     /locus_tag="PLF90_02890"
     CDS             14491..15270
                     /locus_tag="PLF90_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOK56263.1"
                     /translation="MKKRGFTLIELLVVVAIIAILAAMLLPALSKARERARQAVCMSN
                     LKQIWHALWMYTEDHDGWFLPARTPYSDYSDGVVSLQPWFELLGNYGKGYGGTDKKYT
                     PLDYGVYIGVGNYERHTKGKALRCPSERRPFTYTHYHINIWLVGSYTTSSDGYIPDPT
                     YGPYLKKMNKVRNPSVAIWVTDGAAPDQHSISYMYYYYKHSQTGNIYNCFKHINDICN
                     ILYVDGHVEGRSASSLNIAKGQGPNTPLRRGFVLPSGGYNP"
     gene            15287..18403
                     /locus_tag="PLF90_02895"
     CDS             15287..18403
                     /locus_tag="PLF90_02895"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOK56264.1"
                     /translation="MFKRVFVFFFFIIFLCFGDAKLVFYLNFENEEVKDIVGNRVGEL
                     KGGKFVEGKVGKGIYFYGKEKEAECIIFKDLAKDKFFQNFEGGPFTISVWIKPDSTKE
                     HNKQQEILNTAGDIGPGWRLTYTWRTILFRTGTGKRDAEGKGEYWEVKTNPSIDKVNL
                     DEWNHIVVVRNEEGILSLYLNSKKVAESDKKFDIIPSNRFLTVGAYLGGYAYGFKGVI
                     DEVKIYKGALSAEEIFKEYKGDTEKKIKLDGKLDEDIWKKGKVFTNFNLINSTNLSPV
                     QTKVIFNYDTDNLYFAFICDEPNIEKLKNEIKENSLKVYRDDSVEIMLDTDNNKGDYY
                     HFLFNPGGYYGVKFRTQSGFVGSEVKDFKLYTGSLIEKGKWIVEIVIPYSSLTLERIK
                     NDISLNFARNRRVDLPSREESSIAEKGQFHNPFAFIPVKLENIDLSLYAVEFKDINII
                     DTEKKDEKLNVKIKGNLKNLSSKERLLDLELYEEKLGILGKTSLQVLPEREREIILNI
                     NVPEPKEYKFGIDIKDKGRTIYSSIFPLKVTYIPISIDLLKPLYRNSIYSTQKIDEIV
                     INVKINLKEEEIKGTNIEVLIIDKNGKEIKKKLSKSEKETVLSLKIPELKEGEFKIVG
                     RLTKEGKAVYETSIPLYKLPPAKGNEVYVDENLNLVLNGKPILPLIWWAGSPPEEIAK
                     TGADGIICGIGDLDKLTELKQYGEVMLLWGSEQKKYFEGKETLSEEAIKVITEKINSV
                     KDHPNLLFYYLVDEPEGRNISPNLLKEVYELMKKLDPYHPVQITNNTIQGIETYINCA
                     DLFFPDPYVCPLIDGSLTRPMTYIILFMEEIRKSGKGKKFIGITPQVFDYGKSYASRP
                     PYSTRNNRGPTFVEERCMNYLAIVYGAKGFNYYIYGKKEEGHWGAINLPDLRVGMPYL
                     IKEKKSLSDIILLGKDTKEKVKIEDKRIHYTVKEFKGKIYIIAVNVEPVEVNIEINIP
                     ENVKKLKVISEKREINVENGKFQDRFEPYEVHIYTDNLKFEDVIDLKKVEEEIKKEGG
                     WFTYQYKK"
     gene            18400..18543
                     /locus_tag="PLF90_02900"
     CDS             18400..18543
                     /locus_tag="PLF90_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56265.1"
                     /translation="MKFLKIGGCKTDISPPVGYLLCGHLPRNKPSKKNSRPIIFEMCF
                     CFR"
     gene            complement(19230..19451)
                     /locus_tag="PLF90_02905"
     CDS             complement(19230..19451)
                     /locus_tag="PLF90_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56266.1"
                     /translation="MKKKRLKELFCGKGRKYSLTFKNILEELKEIQIGYINVKGLKIK
                     QLNKMTKLQKEILKKLRVELKLKNKLNTN"
BASE COUNT         7732 a         2382 c         3418 g         6287 t
ORIGIN      
        1 ggcaaattct gcaggattta atgattataa agaatttttg aaacatatat tgacagaaat
       61 agaaaaacat gcaatagaaa aaaactggct tccagtaata tttgctcttt atgatgagcc
      121 ggtaagtaaa gaggcaaaag aagcaacaat cagttattgt ttaatctgga aggatataac
      181 aaaaaattta acaaaaataa aaacaacagg atatacaagt atgagttctt caggagaaat
      241 agatgaaact caaattaaat tagcaacttc tgttgatata gtgagtttaa atattcatga
      301 tgagaaagca ttagatgtat tgagagaaaa aggaaccgga tgggcttatt ataataatgg
      361 gaatagattt acctatggaa tatatctatt taaagcaaca aaagaactca atttacatca
      421 tagaattgcc tggcactgga atgtatgtgc tggagaccct tattatgctc ttgattgtag
      481 agaggatgat tatgcatggt ttgtaacaaa tgctaaaaaa gaacttattc cctcaatatt
      541 tttcttaagg ttagcagaag gaattgatga ttatagatat ttacttacac tttcaagatt
      601 gataaatgaa aaacctaatc atacatattc aaaagaggca aaaagaatta ttgatgaaac
      661 gctatcttca ataaaagttg gacagaggac atatccacca actgcataca gagaaataag
      721 atataaagta gcagaagcaa tagaaaaatt gttaaaataa atgaaaaaag aaagggttta
      781 ttttacaaag attagtggtt catatagtcc aaatgagatt tataaaaaag taagacctct
      841 tgaaagtaaa gaatttattt taaatcctca taaaggattt gctacttttc agcattttaa
      901 tggtgaccca ttatttggtg aaggagggga tagagatgat ttaccgatgg atgcagcagg
      961 cccacttgaa tttgaactta caaaaaaaga accagaagtt gtagatggat atcttccaac
     1021 aacaattgct tattgtagat ggttttggaa aatttttgaa ccagaggaag ggaaatttaa
     1081 ctgggatatg gttgagaaat ctttaaaagt tgctgaatta agaggtcaaa gtttgcaagt
     1141 aagattgatg ccatttggaa gtaaaaatca accccaacta cctgaatggt atataaaaat
     1201 agccccaacg agaaaatgta aattttatga aggagtatta gagccggatt atgatgaaca
     1261 aacgtatttt aaatactgga gtagagttat agaagaattc acaaaaaaat ttgcaaatca
     1321 tccaaatctt gaatcatttg acattgcttt tattggccca tggggagagg gcgcaggaga
     1381 aattaaagat gaaacagttg aaaaatttat agatatgtat ttgagatttt ttgataaaaa
     1441 gaaattactt gttaatattg aaggttatca gtttatttat ggaataaaaa agggagtagg
     1501 atggagatgt gactgttttg gagatatgag gaatgcagga gtaaatgttg ttcctgatgg
     1561 acttgggtgg aaccatatgt ttgatgtgta tcctaaatat gtttgtagag caagtgcaca
     1621 ggaagtatgg aaaaatgtac ctgttgtttt tgaaacatgt catactcctt tatggtggta
     1681 tgaaaatgat atgccactgg aatttttaga ttttataatt gaacagggat acaaatatca
     1741 ttgttcagta tttatgccaa aatatacaag aattccaaat gcatataaag aaaaattttt
     1801 aaagtttgct gataaaattg gttataaatt tgtgataagg cagttaaaat ttgagagaaa
     1861 gttgaaagga aaaagaaaat ttaaatatga aatgtggata gaaaatgttg gagtagcacc
     1921 attatataaa aataaatata aacttgcttt taaatttaaa caagggaaaa aagaagaaat
     1981 agttcaatca tctcaggata taacaaaatg gcttcctggg gatatttgga ttgaagaaga
     2041 gttggaatta aatacaaatt ttgaagaagg taagatagat atttatgtag caatagttga
     2101 cagtgaaaca aataaaccaa aaataaaatt tgcaatagaa gggaaaaaag aaaatggttg
     2161 gtattttatg gaacaggttg aaaaatagat ttcaaaagaa aataagtttt aaagcagaaa
     2221 gaaaagcaag aatttggaca atttgattaa atactttttg agggattttt tttaaaaaga
     2281 atattccaga aattgctcca atacaaacac aagggaatag aagtaaattg agtttcaaag
     2341 aataaaaatt tataagtcca agatttacac tgaaggggac tttaaaccag tttaaaataa
     2401 aaaaatacca tgctcctgtt ccaacaaatt ctgtctttgg tagtttcatt gcaagtaaat
     2461 aaattattaa aattggtcct gctgcatttg ccatcattgt tgtaattccg gcaaaaagtc
     2521 ccattattac agcaaaataa aatttttcag gtactttaat ttctttttta ttccaccacc
     2581 agtttagaaa aagaattgta agaattacaa gaccaataaa tttgctcaac tgtttatcat
     2641 ttatttttcc cattaaaaaa tatcctataa ttattccagt tgcggcaaat ggtaaaagac
     2701 gaaggatata cttccaaacc gcttttcttc tataataagt tactgcaaat atatctccca
     2761 ttataagcat aggtaaaatc aaaccagtgg atgcttttgc aggtaaaatc atagcagtta
     2821 aaggaattgc aagaatacca actcctggaa tccctgtttt tgagacacca attaaaaaag
     2881 catataattc cagcaaaaaa agtttaaaat gacttaaatt atatatactt tccatttttg
     2941 cctttaaatt tataatttta tcctctttat gtcaaattga tttttgacaa aaaattttca
     3001 tatattaaac ttatatctgt gaaaaatttt gtttttatta tttttttatt tctaatggag
     3061 caatttataa tggctgaaaa ttttgactgg atgaataatc tgaaaaaaga aagaccgaga
     3121 ttgattttaa atgataaagt tgttgaagat ataaaaactc taataaaaaa tgatgatact
     3181 tgcaaaaaaa tttatgtaaa gataaaggaa aatgcagaaa aaatctgtga tgaacctgtt
     3241 tcaaaatatg aaattccaga tggattgaga ttgctttatg tttcaataaa agttctaaac
     3301 agagtttata cactttcact tgtttataaa attgagggaa ataagaaata tttagaaaga
     3361 ttatggaatg aaatctatac cgcaggaaat tttcctgact ggaatccaaa acattttctt
     3421 gatactgcgg aaatggctca tgcttttgca attgcttatg actggtgtta ttatgactgg
     3481 acagaggacc agagaaagat aatacgagaa gcacttttaa ataaggcatt gaaggtcgct
     3541 ttggattgtt atgaagggaa aattccttat ggctggtgga caaggtcaaa acataactgg
     3601 aatcaggtat gtaatggtgg aattgggatt gcatgtcttt ctcttttaga tgaatatcct
     3661 gaaatatgtt caaaaattct tgataatatt atgaaatcat taccccttgc aatgaaagaa
     3721 tttgctcctg atggtggttg gggagaaggc cctggatatt gggcttatgc aactgaatat
     3781 aattttatct ttcttactta tcttgataac tgtataaatt ataattttga tttctggaat
     3841 tatgaaggtg tatctgaaac aggatttttc ccgatatata taaccggacc tgttgataaa
     3901 acttttaatt ttgcagatag tggtgacgga ccagtaagaa gttcccagtt attttactta
     3961 tcaaaaaaat ttaaaaagcc tgtttttgcc tggtatccaa ttaaataccc aagataccat
     4021 cctcttgatt taatctggtt tacggattat tctgactcgg aaaaagaaaa tttgccatta
     4081 gataaatatt tcagatatgt agaagttgtt acaatgagga cttcatggaa taacagggat
     4141 gctttatttg ttggatttaa aggaggtaga aataatttta atcactctca tcttgatata
     4201 ggcagttttg tttttgatgc ttatggttac agatggatta ttgatttggg tcttgataat
     4261 tataatttgc caggttattt cggtaagaat agatgggatt tttacagatt aagggctgaa
     4321 ggacataata caattgttat caatccttca gaaaaatttg accaggaacc agatgcaata
     4381 tcaaaaatag taaattttgg ttccaaaaat gagtattctt ttgcaatttc tgatttgacg
     4441 gatgcttata gaaaatatgc taaaagtgta aagagaggaa tcttgcttaa caaaaaagaa
     4501 aaatttttac tcatacagga tgagattgaa ttgaaagaaa aaggagagat atggtggttt
     4561 ttacatacag gttgtgatat tgaaataggg gaaagcaaaa aagaagttat tttaaaacaa
     4621 aaaaatacag aattgaaagt taaagttatt tctccgtcag atgctcaatt tttaataatg
     4681 gatgcaaaac cactttcttc ttctccaaat ccaacaggtc agaatgaaaa caaaggaatt
     4741 aaaaaaattg ctcttaattt taaaaatact gataatttga ggatttcagt aatattagtc
     4801 cctttaaaac cgggtaataa attttctcct tttacccctt cattaatccc attaaatgat
     4861 tggcaataga aactgactta aattatggaa aaaataatac atataatctg gacaatggat
     4921 tgtgagtttc ctaaaagtcc ttttggtggt cctgaaaact gggaaattgc tgaaaaatct
     4981 gttaatggat ttcttgaagt actgacaaag cactgtcaaa aagcaacttt ttttgtaact
     5041 ccagaagtta ttcagaaaaa atttaatgta ttttcagaaa aaaataaaaa ttttgaaatt
     5101 ggtatgcatt tacatccatt tgatgacagt aataaaaaga aattatatat gggacagatg
     5161 aggaaagaag aacaggaaaa ggtattaaaa gaaaatagag aaaaatggat acaatcagct
     5221 ggttttgaac ccagaagttt tagacccgga aatttttctg caaatgatta tacctttcct
     5281 ttacttttaa aactcggttt ttttcaggga agcgtatcta gtccttatcg taaaatggaa
     5341 aaatttttta ctgattggga aggtgcttta cttgacccac atcatacgaa tgaaaaatca
     5401 agattgattg aggggaaatt agattttttt gaagtcccag taactgttga cccatttaga
     5461 aaaaaaagga tagatggaac ttcttatgaa ttgaggattg aatggggtga tatttcagaa
     5521 catatagtga ctattgaaaa tgttttgaaa agattgaaaa aagaaaatcc acctgtaaaa
     5581 agtattgttt caattaccca taatactttt gattatactc tgaaaaattc aaaatatcgt
     5641 tctactcttg aaaaacttat tttattcatt caggaattaa ttataaaaga aaacttcaca
     5701 cctttatcca taacaataga agaacttcat aagaaagtag atggaaagga tttatgaaat
     5761 ttacaagtta aataaattgt agtaaaatca atagcgaaga aatttcaatt taaaatcaaa
     5821 aggaggcaaa atgaaaaaaa gtaaaaaaaa ttatttgaaa attcctgttg aacatgttga
     5881 tattaaaaaa atagatgcaa ttccaatagt tgaaatgtat tccagaatgt ctttttcagc
     5941 aagaactctt gcatatggtg ctttacttta tgagaaaatg cttaaagatg aggaggtaac
     6001 tgtaatttta actcttgcag gttccctttt cagtgcagga cttaagaatg ctgtatgtga
     6061 tctggtaaga aataatcttg ttgatataat tgtttctaca ggagcaataa tagttgacca
     6121 ggattttttt gaagcattgg gttttagaca ttatatagga agtcctgatg ttgacgatag
     6181 agaacttaat aaacttcata tagacagaat atatgatact tatattgatg aagacgaatt
     6241 aagaatttgt gatatgactg ttgctgaaat tgcagaaaaa cttgaaccaa aaccttattc
     6301 atcaagagag tttataaagg agatggggag atatttaaaa gagaaaggaa ttaaaagcga
     6361 tgattcagtt gtacttgctt gctatgaaaa aaatgttcct atttttgttc ctgctttttc
     6421 agattgttct gctgggtttg ggcttgttta tcatcaaata aaacatccag aaaaacatgt
     6481 ttcaattgat tctgtgaagg actttaaaga acttacagaa ttgaaaattg ctgcaaatca
     6541 aacaggggtt gtaatgcttg gtggaggggt tcctaaaaac tttacacagg atatagttgt
     6601 ctgtgccgat gttttaaata aaacagttcc aatgcataaa tatgcaattc agattactgt
     6661 tgcggatgtt agagatggag cactttcaag ttcaacatta aaagaagcac attcatgggg
     6721 taaagttgat tttccaaata cagaaatgat ttattctgaa gttacagttg catttccaat
     6781 aatcgctgcc tatgtatatc aaaaggtgaa aaatagaaaa ccgagaaact ggaataaatt
     6841 ttttgaaaaa tgattttaaa tgaaaaacat aactatttct atatatttta ttttaacaac
     6901 atttcttttt gcccaaagta aaaatggagg agaaatggat aaacaaatac cttctttatg
     6961 tgaagtatat aaagattatt ttccaattgg agcagcagtt aacttaaaaa ctgtagtttc
     7021 tcatgcggat ttactgaaaa aacattttaa tagtataact cctgaaaatc atacaaaatg
     7081 gggtttaatt catcctttac ctaataaata taattttgaa gatgcagata aaataataaa
     7141 tttcgcaatt gaaaacaaaa tgaaaataag agggcataca cttgtttggc atattcaggt
     7201 tcctgaatgg gtttttaaag atgaaaaggg gaactggata gataaggata cacttttgaa
     7261 acgactttat gaacatatta aaactgttgt tggatattat aaaggtaaag tttatgcatg
     7321 ggatgttgta aatgaggcaa tttcagacaa agaaggagag tttttaagag atgagccatg
     7381 gtacaaaatt ggaggagaag aggttataga aaaagcattt atttgggctc atgaagtaga
     7441 cccagatgct attttgtttt ataatgatta taatctggaa ttaccatcta aaagagaaaa
     7501 ggcatatcaa ttgattaaaa aactgaaaga aaaaggaatt cctgttggag ggataggcat
     7561 tcaggcacac tggggattat atggaataac gccagaagat ttagaagaga gtattaagaa
     7621 attctctaca cttggtgttc agatacagat tacagagttt gacatttcaa tatatcttga
     7681 taaaaatgaa aaatttaatt ccattgaaga agttcctgaa gaaagaataa aaaagcagat
     7741 agaactttac aaaaaagtat ttgaaatttt aagaaaacat aaaaatctaa tcactggtgt
     7801 tactttctgg ggagttgcag atgatattac atggcttgat tatttccctg ttaaaggtag
     7861 aaaaaacttt cctcttttat ttgacattta tcataaacct aaaaaagttt tctgggaaat
     7921 tataaatttt tgattttatt ttttcaaaat ttatgaagaa ttaagtttat tccccagtaa
     7981 ataaaaatta aaaagcctaa aaatttaaac aaaactttca cctttgaatg aaaaaggatt
     8041 ttctgtaaaa aatatggaat aaaaccaatt attaacagca cagggaaaat tatagaactg
     8101 agagcaaata gaaaaccagc agaaaaaccg aaaaaaatac ctttatttaa atatgcaata
     8161 taaaaaatta atcctattaa aggagcgcat ggagttattc cttctataat tcctaaaaat
     8221 ataggtgtag atgttttaaa aacttttaca tatttttttg aaggaaataa aaaaagtata
     8281 cctattaaaa taagaattaa acctgatata tatgaaaaaa cttctttttg aataatactc
     8341 tgaatataat ttccaaaata agaagcaagt cctccaataa atccataaat aaaaatcttg
     8401 cttatgctga ataaagaagc aattttgatt cctcctttcc agttatctga ttcttttata
     8461 accacaggaa aaagtaaaaa acaacaatga aatccacaaa aacttattcc tacaagaaag
     8521 ccggacaaga aaaattgaag taaaaatttc atgattaaat tttaatcttt atttttaaaa
     8581 gcagtcaatt ttaaaaaaat aaaaatttat ttttgaaaaa tcattatgat atactataaa
     8641 aaaaaggaaa ttaaaatgac aagggatgat ggttttgaac ttttgaaaaa atatatttca
     8701 aataaaaatc tcataaaaca ctctcttgcg tgtgaagcaa ttatgagaaa actggctaaa
     8761 tattttggtg aaaatgaaga aatctgggga cttgctggtt tacttcatga ccttgattat
     8821 gaatatacaa aagataatcc tgaaattcat ggtttaaaaa ctattgaaat gctgggtgac
     8881 agtgtaaatg aagaaataaa gaatgcaatt cttgctcatg ctgaaaagaa aaatccagaa
     8941 acaaaaattg aaaaatcttt atatgcagtt gaccctgtta gtgggtttat tgttgcctgt
     9001 gtacttataa gacctgaaaa gaaacttgaa attatagata ttgaattttt gaaaaataga
     9061 tttaaagaaa aaagttttgc aaagggagcc aacagagaac agataaaaac atgtgagaca
     9121 ctgggaatta aacttgatga atttctttct ctatcacttg aaagtatgaa aaaaattgct
     9181 gtagaacttg gcctttgagt tgacaagaag gatgaatttt tctataataa attaaaatta
     9241 aagccacctt agctcagcgg tagagcaggg cacttgtaat gctcaggtcc ggggttcaaa
     9301 tccccgaggt ggctcttaat ctaatttttc aaaatggtca agtcagataa atggattaaa
     9361 aaaatggctc agaaaggtat gatagagcca ttttttcctg aaaagattaa aaaaggaatt
     9421 tcttttggac tttcctctta tggatatgat tttacaattt caaatgaatt tttgattttt
     9481 aaaggtaaaa ttgcatctcc aaaacaaata acagaaaatg attttgaaaa atataaagga
     9541 aatgtttgca agattcctcc aaatagtttt gttcttggga aaagtgttga atattttaga
     9601 attccaaggg aagtaattgg aatttgtttt ggaaaatcta cttatgcaag atgtggaata
     9661 attataaatg taacccctct tgagccagaa tgggaaggat ttattacaat acaaatagca
     9721 aatctaactc ctgttgaagg tgaagtttat gctggagaag ggattggcca ggttatattt
     9781 ttatctgcag atgaaatatg tgaaaaatct tataaagacc attctggaaa atataataat
     9841 gcaaaagata tacaacttcc taaaatataa gtaaaattat ttcttaaatc ccaagtaaat
     9901 acttgctcaa ctacaggtat aaaacattgg aatttgaaaa attccaagtg ttttttctgg
     9961 cgttttttgc ccagaacaaa taaaaatacc ttaagtcccc ctcaaagatt gagggggtgc
    10021 tggtgcgacg aataaggagc accgattagc acgggtggcg agatggtgag ccacctattt
    10081 aggggagttg atacctattg aggattttaa aggggtttaa acccctttaa gaaagaaccg
    10141 tagttgacaa taaaaacaaa atataacaaa ataaaaaagt aaatgggatt cagtaaagat
    10201 ttacaatcct gagtttaatt atgttataat aactttatgg aaaagattat atttttttta
    10261 atgggtgtaa aaatctgcgt taaaaaagga gatattcttg aggaaaaagt tgatgctatt
    10321 gtaaatcctt caaatacaaa ttttactatg ggtggtggac ttgcaaaaaa aataaaagaa
    10381 aaaggtggaa atgaaattga aaaaatggca aagcggaaag gaagagtaca gcccgggtat
    10441 tgtatttata cagattccgg taaattactt tcaaaatata taattcacgc agtaacaatg
    10501 gatatggatt ttaagtcaga ttatgatgta ataaaaaaat gtatggaaaa ctcttttaaa
    10561 ctctgtaatg atttgaagat aaaaactatt tcttttcctg ctattggttg cggaacagga
    10621 aaacttgata gtaaaaaaat tgcagagatt atggtaaggg aaacattaag atatttatcg
    10681 ggtaaatttg aaacagatga aataaacttt gtacttttta aaaagagaga ttacgagaat
    10741 ttttgtaatg tttttgaaga gtatttgaaa aatttgacta aaaagacgta taaaaatcca
    10801 attcccacag ttgatataat aattgaatat aatgatggaa tagttttaat agaaagaaaa
    10861 aattatcctt ttggctgggc aataccaggg ggttttgttg aatatggaga aagttgtgaa
    10921 gaaactgcaa ttagagaggc aaaagaagaa acaggacttg aacttgaaga tttgagacag
    10981 tttaagacat attcaaaacc agggagagac ccaagatttc atacaataac aaccgttttt
    11041 acagcaaaag gtaaaggata tttaaaaagt ggagatgatg caaaaaatgc ttcagttttt
    11101 aatgaaaaaa atttacccga aaatattgct tttgaccaca gggatgtact tatggaatac
    11161 ttttatttta aaaacaaatt aagaaattaa gaagttcata agttaataag ttcaggagtt
    11221 aaaagtgaaa aaaataattt taagtggtat gagaccaacc ggacctttac acataggaca
    11281 tttatttggc gctttaaaaa actggaaaaa attacaggat gaaggatata aatgtttcta
    11341 tatgattgct gattatcatg ctttaagtac agaatatgca aatccccgta aaataaaaga
    11401 atatgtagaa gaaatggcta ttgattttat ctcttctgga cttgacccgg aaaagtcaac
    11461 aattttcatt caatctcttg tacctgagca tactgagtta catttaatat tttcaatgat
    11521 tgttcctatt ccttatcttg aaagaaatcc agtttataaa gaacagatag aagaattgaa
    11581 agataaggaa ctgcatactt atgggttttt aggttacccg gtattacagg cagcagatat
    11641 tttgatttat aaggcaaact ttgttccaat agggattgac caacttcctc atcttgagtt
    11701 gacaagagaa atagcaagaa agtttaatta tttatatgga gaaacattcc ctgaacctga
    11761 acctctttta acagaaacac caaaaattcc aggaacagat ggaagaaaaa tgagtaagtc
    11821 atataataac tgtatttttt taaaagatag cccagaaatt atagaagaaa aggtaagtaa
    11881 aatgtttaca gaccctaaaa gaatttacag aagagaccca ggacatcctg aaacctgtcc
    11941 tgtttttgcc tatcataaaa tttttaatga aaaaagagta ggggagatag aaaaggattg
    12001 taaaggtgct gtaattggat gtactgattg taaaaaagaa cttgggaaaa agattgttga
    12061 atatcttgaa gaaataagaa aaaaaagaga gaaaattgag aaaaataaga aagatttgat
    12121 ggagatactt ttggaaggaa gcaagaaagc aagagaaaca gcaattaaaa cgatttatga
    12181 agtaaaagaa aaaatagggg ttaaatatga aaaataatat tgaaaaactt gtaagaaaag
    12241 aattaaaaga aattgctcct tatgttccgg gtaaacctat tgatgaagtt aaaagaattt
    12301 acaatattga cagaataata aaacttgctt caaacgaaaa tcctcttgga tattctccga
    12361 aggtaaaaga agtaataaaa gaaaatcttg atgatataaa cagatatcct gatggttctg
    12421 gatattatct gaaaaaagaa ctggcaaatt ttcttaatat atctgaagat gaaataattc
    12481 ttggcagtgg ttcaagtgaa gttatatcac ttgctattga agcatttgta aatcctggtg
    12541 aagaagtaat ttatccattt ccatctttca ttatttacag aattctggtt ttaaaggttg
    12601 ggggagttcc cgttgaagtt caccttgaaa atgatttttc ttacaacatt gatagaattc
    12661 tggaaaaaat taattcaaag acaaaagtga taattttatg taatccaaat aatccaacag
    12721 gaacaatttt atataaaaac caacttgaaa gatttctgaa taaaattcct gataatatta
    12781 ttgtgatttc tgatgaagca tactttgaat atgttgagga taaaaatttt ggaacgtgtt
    12841 ttccatatta caaagaaaaa aatattgtaa ttacaagaag tttttcaaaa atttatggac
    12901 ttgcttcttt aaggattgga tacggaatag ggaaaagtga gattttaaat tatatggaaa
    12961 gaattagacc cccttttaat acaacaggta ttgcacagat tgctgctata actgcattaa
    13021 aagatatgga ttttgttaaa aaatcaattg aaaataatat aaaaggaaaa aagttccttt
    13081 atgaaaattt taaaaaactg gggattgatt atatacctac tgaagcgaat tttatacttt
    13141 gcagatttaa aggagatgca acttttatag taaaagaact tgaaaaggcc ggtgtaatag
    13201 tacggggaat gaaaagtttt ggactatcaa acgaatatgt cagaattact ataggaacag
    13261 aagaagaaaa tagaattctt atagaaaaac tttcaaaatt gatttaattt ctttattcaa
    13321 aaagaagaac tgcttttata tatccttcgg gtttttcaag tgttgttctc attgctttat
    13381 ctatttcttt aactgaaaat ctgtgtgtaa tcagtttttc aaggttaaat tttctatttt
    13441 caagaagttt tattcctctt tcaaagttat ccatatttct tttaattccc attacaggag
    13501 aagggtttaa tatatgcaga ccttttgaat gccacaaacc tgcattcaca gttctttctc
    13561 cgtgatgcca tgaaaaaatc gcaagtttac ctgctttatt tattatttct tccgcgagat
    13621 ttaaagcttc ctgaactcct gcacattcaa ttacaatatc aaatctattt ctgtactttt
    13681 caatatcaaa tcctttctta atattcactg tttcagttgc tccatactct tctgcaagtt
    13741 taagattata ttcttttata tcaaagacga caatttcttc aacaggaaaa ccggaaagtc
    13801 cttgaagatt taaaagtccc ataaaaccag caccaattaa agcaatttta tccccaggtg
    13861 aaacagaata aaaattaata gcatttaaga cacaggcaac tggttcaact atatattttg
    13921 cagggtcatc aacttttata gaaaattttt taacatcttt ttctttcatt ttctggtata
    13981 aactccagtt aaagcagacc acataatcac cttctttgat atttttaact tctttccctt
    14041 tttcaactac aactccaatt ccttcatgtc ctataatcga attttctggt aatttttcta
    14101 taccagcaaa aagagatgat tcccacatac atataccatt tgcaagacat ttaatcataa
    14161 cttcgtcatt ctttacatct tcaatttcat gttcccttat ttcaaacttt tctttatcca
    14221 tataaaaaac actatatcct ctcattttca ctcctttttg aatttatttt agcataacaa
    14281 aatttattaa aaaatttttc ctattcccac ctttgaggtg ggaaatggaa agcaagaaaa
    14341 aatttgggat aactccagca caatatagaa aaattatata aaagagcaaa aaattacaaa
    14401 aaacagcaaa aaattacata aatttctctt gactataatt aaattttaaa gttaaattta
    14461 taaagatgaa aataaaaaag gaggtgtggt atgaagaaaa gagggtttac tttgatagag
    14521 ttgcttgttg tggtggcaat aatcgcaatt ttggcagcga tgttacttcc agcattaagt
    14581 aaagcaagag aaagggcaag acaggcagtt tgtatgagta atttaaaaca gatctggcat
    14641 gctctatgga tgtatacaga agaccatgat ggatggtttt tacccgcaag aacaccttat
    14701 tctgattatt cggatggagt tgtttcatta caaccttggt ttgaattact tggaaattat
    14761 gggaaaggat atggaggaac tgataaaaaa tatactccat tagattatgg tgtatatatt
    14821 ggagtcggaa attatgaacg tcatacaaaa ggtaaagcat tacgttgtcc aagtgaaaga
    14881 agaccattta cttatactca ttaccatata aatatttggc ttgttggaag ttatactaca
    14941 agtagcgatg gatatatccc tgatccaact tatggtcctt atttaaaaaa gatgaacaag
    15001 gttagaaatc cttcagttgc aatatgggtg acagatggtg cagctcctga tcaacatagt
    15061 atatcctata tgtattatta ttataaacat tcccagaccg gtaatattta taattgtttt
    15121 aagcatataa atgacatctg taatatttta tatgtagatg gacatgttga aggaaggtca
    15181 gcaagttctt taaatatagc aaaaggacag ggaccaaata ctccattaag aagaggattt
    15241 gtattaccta gtggtggtta taatccataa ataacaggga ggagatatgt tcaaaagagt
    15301 atttgttttt ttctttttta ttattttttt atgttttgga gatgcaaaac ttgtttttta
    15361 cttaaatttt gaaaatgaag aggtaaagga tatagttgga aatagagttg gagagttgaa
    15421 aggtggaaaa tttgtagaag gaaaagttgg aaaaggtata tatttttatg ggaaagaaaa
    15481 agaagcagaa tgtataattt ttaaagattt agcaaaggat aaattttttc agaattttga
    15541 aggaggacca tttacaataa gtgtatggat aaaaccagat tcaacaaaag aacataataa
    15601 acagcaggaa atattaaata cagcaggaga tataggacct ggatggcggc ttacatatac
    15661 atggaggacg atactattca gaacagggac tggaaaaaga gatgcagaag ggaaaggaga
    15721 atactgggaa gttaaaacaa atccaagtat agataaggtt aatcttgatg aatggaatca
    15781 tatagttgtt gtaagaaatg aagaaggtat tttatcttta tatttaaata gtaaaaaggt
    15841 tgcagaaagt gataaaaagt ttgatattat cccttcaaat agatttttga cagttggggc
    15901 ttatcttggt ggttatgctt atggatttaa aggagtgata gatgaagtaa agatatataa
    15961 aggtgcttta agtgcggaag aaatttttaa agaatacaaa ggagacacgg aaaaaaaaat
    16021 taaacttgat ggaaaacttg atgaagatat atggaaaaaa gggaaagtat ttacaaattt
    16081 taatttaata aattcaacaa atttatcacc tgttcaaact aaggttatct ttaattatga
    16141 tacagataac ctctattttg cttttatatg cgacgagccg aatattgaaa aactaaaaaa
    16201 tgaaataaaa gagaactcat taaaagtata tagagatgat agtgttgaga taatgcttga
    16261 tactgataat aataaaggag attattacca ctttttattc aatccgggtg gttattatgg
    16321 agttaaattc aggacacaaa gtggatttgt tggttcagag gtaaaagatt ttaaattata
    16381 tacaggaagt ttgatagaaa aaggtaaatg gatagttgag atagtcattc catattcttc
    16441 tttaacactt gaaagaataa aaaatgatat atctttgaat tttgcaagaa atagaagggt
    16501 tgatttgcct tcgagagaag aaagttcaat tgcagagaaa ggacaatttc ataatccatt
    16561 tgcttttatt cctgtaaaac ttgagaatat tgacctatca ttatatgctg ttgaatttaa
    16621 ggacataaac ataattgata cagagaaaaa agatgaaaaa ttaaatgtga aaataaaagg
    16681 aaatttgaaa aatttaagtt caaaagaaag attgctggat ttagaattat atgaagaaaa
    16741 acttggtatt ttagggaaga caagtttaca ggtattacca gaaagagaaa gggagataat
    16801 tttaaatata aatgttccag agccaaagga gtacaaattt ggtatagata taaaggataa
    16861 agggagaaca atttacagca gtatatttcc attaaaagtt acatacatac caatttcaat
    16921 tgacctttta aaaccattat acagaaattc aatatattca acacagaaaa tagatgagat
    16981 agttataaat gttaagatta acttaaagga agaggaaata aaaggaacaa atatagaggt
    17041 tttaataatt gataaaaatg gtaaagaaat aaaaaagaaa ttgagtaaat cagaaaaaga
    17101 aactgtttta agtttaaaaa tacctgaatt aaaagaagga gaatttaaga tagtgggaag
    17161 attaacaaaa gaaggaaaag cagtttatga gacaagtatt ccattatata aattaccacc
    17221 agcaaaagga aatgaagttt atgtagatga gaatttaaat cttgttttaa atggaaagcc
    17281 aatactacct ttaatatggt gggctggttc cccacctgaa gaaatagcaa agacaggagc
    17341 agatggaata atatgtggaa ttggtgacct tgataaatta actgaactaa aacaatatgg
    17401 tgaagttatg ttattatggg gttctgaaca gaaaaaatat tttgaaggaa aagaaacatt
    17461 atcagaagag gcaataaaag ttataacaga aaaaataaat tcagttaaag accaccctaa
    17521 tctgttgttt tattatttag ttgatgaacc agaagggaga aatataagtc caaatctttt
    17581 aaaagaggtc tatgaactta tgaaaaaact tgacccatac catccagttc aaattacaaa
    17641 taatacaata caaggaatag aaacatatat aaattgtgct gatttatttt tccctgaccc
    17701 atatgtatgt ccacttattg atgggagttt aacaagacct atgacatata taatcctttt
    17761 tatggaagaa ataagaaaat caggtaaagg caaaaaattt atagggataa ctccacaggt
    17821 atttgattat ggaaaatctt atgcttcaag accaccatat tcaacaagaa ataatagagg
    17881 tccgacattt gtagaagaga gatgtatgaa ttatcttgca attgtttatg gagcgaaggg
    17941 ctttaattat tatatttatg gtaaaaaaga agaaggacac tggggagcaa taaatttacc
    18001 agatttaagg gttggtatgc catatttaat aaaagagaaa aaaagtttga gcgatattat
    18061 actacttgga aaggatacca aagaaaaggt taaaattgaa gacaaaagaa tacattatac
    18121 tgttaaagaa tttaagggaa agatatatat aatagcagta aatgtggagc cagttgaagt
    18181 gaacatagaa ataaatatac cagaaaatgt aaagaaatta aaggtaatat cagagaaaag
    18241 agaaataaat gtagagaatg ggaaatttca agacagattt gaaccttatg aagttcacat
    18301 atatacagat aatttaaaat ttgaagatgt tattgatcta aaaaaggttg aagaagaaat
    18361 aaaaaaagaa ggtggatggt ttacttatca atataaaaaa tgaaattttt aaaaattggt
    18421 ggatgtaaaa cagatataag tccaccagtt ggatatttac tttgtggaca tttaccaaga
    18481 aataaaccat ccaaaaaaaa ttcacgaccc attatatttg aaatgtgttt ctgtttcaga
    18541 tgaaaaaaat agggttttta taatcacatc cgatttaatt ggttttactc ccgattttgt
    18601 caattcatta aaaaaagaga tttataagga actaaaaata gaaaccatca aaaatacttt
    18661 taactgcatc tcatacccaa aagagtcgta aaattaaatc tttcaaaatt ttttttaatt
    18721 ttaaaaaatg cttttgaaaa tagaaaatat gaaaagggag aggtttttga cagaagaatc
    18781 cagaaagtaa tagaatttat aaataccaat tattccagaa aatttctgaa aaagagatct
    18841 gtcaatatgt aggcctttct caatattatt ttgcccgtct ttttaaaaaa tcactggttt
    18901 aaattttaaa aaatatttaa ttgaaaaaag attatttgag gcagaaaaac ttttacaaaa
    18961 taaagaatta aaacttgaga caattgcaaa aaatacaggg ttttcttctt tatcattatt
    19021 tttaaaagaa ttcaagaaaa aatttggtat aactccggca aaatatagaa aaattatata
    19081 aaagagcaaa aaatgacaaa aatttctctt gacaataatt aaattttaaa ggtaaattat
    19141 ttacaaagat gaaaataaaa ccgaacttac caatttcaaa aacaatataa accattgaga
    19201 gacaaaagat ttatttgaaa tttagttttc taattagtgt ttaatttgtt tttcaatttc
    19261 agttcaaccc gcagtttctt caatatctct ttttgtaatt ttgtcatttt gtttaattgt
    19321 tttattttta gtcctttcac atttatatat cctatctgta tctcttttaa ttcttcaagt
    19381 atatttttaa aagtaagtga gtatttcctc cctttcccac aaaataactc ctttaatctt
    19441 ttttttttca tctcaactac tatataatat gaaagcatac aaataaatgc atgggatgtt
    19501 attctttttt ctttatagtg gtatatcggt cttatatatt taatagtgtt gtctttataa
    19561 tcctaaatgc cctatccaca agtgataaac ttttataatg gtcaattatc tgctcttttg
    19621 taaattcttc ttttaaactt gtatctatta cataacatcc taatagtttt tttgtaaatt
    19681 ctaactggtt tttgtcaatc tgaaatctct tctggtaatt tataatctct acaaagtttt
    19741 tcataaggta tcatttctat tgtggaatga atccttttcc tgttatatat ccactgccat
    19801 cttaacaaca aaattcctc
//
LOCUS       DAOSAT010000020        19455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_1970_length_19455_cov_4.815464, whole genome shotgun sequence.
ACCESSION   DAOSAT010000020 DAOSAT010000000
VERSION     DAOSAT010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19455
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1970_length_19455_cov_4.815464"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1767
                     /locus_tag="PLF90_02910"
     CDS             <1..1767
                     /locus_tag="PLF90_02910"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOK56267.1"
                     /translation="IWFADIYNSEIYIDSFEGFKKDKEDKIFLYNFRENSKKIGRYDK
                     FEITFEIPYIFENPFKPDLKIKGVFTSPSGKIYETFAFFYHDYLRTIYDEGEKLIPYG
                     KSEWKIRFTPCETGSYTYKIKVLKDNEENEIYKGNFEVYESNKNGVVNWDKNDRYYLS
                     FSNSKFFYPIGHTLRSPDDERSPYNYEFTYRKGLGTFAYDKYFKKMAENGENYARVWM
                     GVWWVGIEWNYNYAPHYKNLGRYSLENSWKLDYICDIAEKYGIFMDLTLINHGQFSIN
                     PDAEWWDNPYNEINGGFLKSPDEFFVNEKAIEYFKQRMDYIVARWSYSPNIVFWEFWN
                     EVDLTGFYSSEKVRYWHKVMYPYLKSIDPYKKPISTHYCRREDDPMVWIIPELESLVG
                     NAYHNLIVDSIRDYYEKRKFYGKPMFVNEFGVGKDRFFLEDNLHGGIWSSSMMPMFGV
                     ALFWWWPFIDHFDLYFHYKALSKFWENEDRRNKFLQVSDGKIRGKDVDFVGIQNKEEG
                     FFWIFDRKIFNSQLKRIKPVKIENAVLYLKGFLPGKYKIEFWDTYKGEIVKEMFYENK
                     KDIEISIIPFEKDIALKIKRQK"
     gene            1764..3755
                     /locus_tag="PLF90_02915"
     CDS             1764..3755
                     /locus_tag="PLF90_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56268.1"
                     /translation="MRNIYLFFLILQFFVFSEYRYLLNLSCENKKLLETFKIASFNFI
                     SGKEKYDDIKIIDENGKECEISGVNFLKDEMEIFFKPTDSINYKVFIGYSEKNRLYKE
                     FKKGNILIDDYIFPDSIKSGVWLWTEKSLSGIYSHTGTAGFSFHRVSFPKQFSIGSDD
                     KIISYLFIEEENAPEEIIIEVISRYRRHYYFSFGKDVINMKGMNKEKIGELPEIGKWT
                     KIEIPLLKIREKNIEGIGFYNDKGRVYWDMTSINEIPVKMKIRKVENLKSPNTPYFNY
                     EIEGPFTIDNKKITILKLDTSCFLSDKYLWEIDGKVYTGEKIVVKLEGEKKEINIKLK
                     TEKNKISGEFIHKISLGDGKEIKTIFNILPFENFIYEDEKLYIPIRVENISDIPLVLT
                     LASQNNKNEFYFLPGKENGKTMTLFLNPENEIKIGIYFYDAKLAEKTINVVDLNEKDF
                     FVDGVFLKKNNSYLIFKIPEYTNIKKTTGFSKIIFIGDFPDELIQFFKEKGIEVLNIS
                     EVKYVNYLFNEFKFIQEIQDKINSDSFVVLFPFINSLLRRHRINEWEKVYDAILYLLS
                     QKNSNIIVVSPFPSYPYLEVLQPYREKIKKISEKRNFYFLDLYKIFSEIENGERFFKM
                     NEYVYKNLPDKEGFEIIINEIKKIIENEKNFNNQRGSTR"
     gene            3715..4608
                     /locus_tag="PLF90_02920"
     CDS             3715..4608
                     /locus_tag="PLF90_02920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOK56269.1"
                     /translation="MKKILIIREGALGDLILTFPVFFSFKKNGYRVFAGGKGIYKNFI
                     EKYGNVEKFIPLDSSEYLFFFERENTKLKGFLKDFDIIIAYTDEKEIIGENLKKNFMN
                     KIIFHPVKKEKLNIHITDYLMKPVKEIFNKNLYFSINLNLKNKGEFFLIHPGSGSKDK
                     NWGKEKFLKLSENLKNVKLLLGPAEENELSFWKRNFKGEIFFNLDLEDIIKIAERTIA
                     YIGNDSGITHLFSITGVKTIAIFGPTSPFIWGPRGKNVKIVYKNLECSPCVYEKMKNC
                     TDKKCLEEIKVEDILSFISDG"
     gene            4654..5436
                     /locus_tag="PLF90_02925"
     CDS             4654..5436
                     /locus_tag="PLF90_02925"
                     /inference="COORDINATES: protein motif:HMM:NF020416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOK56270.1"
                     /translation="MEKAVIFDGSKGKLFGVIHIPEKEKSPGIVLFHGFTGNKCESHF
                     IFTKLARKLCREGFSVLRFDFYGSGDSEGNFYEMTLETEMKDGEKALEFFKTFDFVDR
                     EKIGVCGLSMGAVTAVYVVHKFKEVKGLCLWSPLAFPKIIQKKILTKKIREKIEKYGK
                     CYIPGMGHYIGKGFIESLGKIDMKEYVENYRGNVFIIHTKDDLSLDLKNALFYFEKFH
                     KNASNLKMLVLDKGGHVFTTEESENTVIDETTEFFKECFKKE"
     gene            5439..5951
                     /locus_tag="PLF90_02930"
     CDS             5439..5951
                     /locus_tag="PLF90_02930"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOK56271.1"
                     /translation="MKKEIKYIDVMEETIEALKEGRVLVCSVDKNNKPNVMAIGWGSI
                     GIIWGEPIFFIFIRPSRYTFNLIEKTEDFTVNIFEEKYKEIVNWCGTVSGREYDKFKE
                     KNLTPLKSKYVKSPVVKESIISFECKVIARMDVKPEMIDKEIKERMYKSGDYHRVYLG
                     KILASYKNFT"
     gene            6007..6294
                     /locus_tag="PLF90_02935"
     CDS             6007..6294
                     /locus_tag="PLF90_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOK56272.1"
                     /translation="MPTYEYECKNCGYKFEKFQKMTDEPLNICPECKKESLRRLVGVG
                     GGVIFKGPGFYTTEYRSEDYKRKEREEKGLSSTSSGSCSSCSASSCSTCKK"
     gene            6415..6873
                     /gene="nrdR"
                     /locus_tag="PLF90_02940"
     CDS             6415..6873
                     /gene="nrdR"
                     /locus_tag="PLF90_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOK56273.1"
                     /translation="MKCPYCGYLKDKVVDSREREDGLLIRRRRICLKCKRRFTTYEEI
                     DLKPLIVVKKDGRRERFNREKLIIGIQKACEKRPISTDKINEIVEDIEKELRQKYENE
                     VTSKEIGKSVIKKLKKLDKVAYIRFASVYQEFEDIEQFLKEIEKIGGKNV"
     gene            6866..9904
                     /locus_tag="PLF90_02945"
     CDS             6866..9904
                     /locus_tag="PLF90_02945"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOK56274.1"
                     /translation="MFKKIVKRDGRIVDFDSTKITNAILKAGQATGEFGIDVAKKLTV
                     KVLDLALEIIKDKIPTVEEIQDIVEEVLLLSPYKKTAKAYIIYREQHARIREITTKAS
                     LDLVDQYLNQIDWRVKENSNMSFSLQGLNNYVSSEVTKTYWLNNIYPPEIRKAYIDGD
                     FHIHDLGSLSVYCVGWDLQDLLLTGFKGAEGKVESSPAKHFGSILGQIVNFFYTLQGE
                     SAGAQAFSNLDTLLAPFVRYDGLSYKEVKQAVQEFLFNLNVPTRTGFQCLSEDTEILT
                     PDGWKKYDQVKKGDTIYTFNIKNGIIEKKEVKHVFVREYEGVMYNLKNRNQDQLISPG
                     HRVVRRIFNTDRFALEPIEKVIELMSPVVIPVKGENINEEYDIEDSKIKLLAWILSEG
                     SIEKQGSHRVSIYQSEEKHPENYNEIIDLLKINELEYSIRPQSSLGICTHIRLKSKPS
                     KVIHNLLGSEKKQFPSFLLKLSKKQAKLFIDTYIKGNGWEEKFRKRITVTDKEAMDII
                     TGVAVLAGYNFNVKEREVGGISKKTQYIITLTETQYDYIQEIKPVEYKGIIWSVNTEN
                     ETVIARRNGQIFITGNTPFTNLTFDLKVSEVYKDQPVIIGGKIQDKTYGEFQKEMDMI
                     NKAFFEVMLEGDAKGRVFTFPIPTYNITKDFDWKNETLDLLWEMTGKYGIPYFSNFIN
                     SDMKPDDVRSMCCRLRLQTDELKKRGGGLFGANPLTGSIGVVTINLPRIGYLSKTKEE
                     FKSRLNDLMEVAKESLEIKRKVLERFTDSGLYPYSRFYLRNVKERFGKYWVNHFSTIG
                     IIGMNEACLNLFGKDIGTEEGINFAIEIMEFMREKLIKFQESTGNFYNLEATPAEGTS
                     YRLARIDKNKYPDIIVANEKECRNGAEPFYTNSTHLPVNYTDDIFEVLEKQDKLQSLY
                     TGGTVIHIFVGEKINSKEGIKKLVKTICENFTLPYFTITPTFSVCPEDGYLNGEVYNC
                     PKCGKTTEIYSRIVGYLRPVSQWNKGKYEEFKIRKTFVLK"
     gene            9901..10590
                     /locus_tag="PLF90_02950"
     CDS             9901..10590
                     /locus_tag="PLF90_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOK56275.1"
                     /translation="MKIGAIQKTSLIEFPNILSCIIFTQGCNFRCPYCHNPELVLPEK
                     FGEIIPEEEIFDFLNRRKKYLEGVCITGGEPSIQPDIIDFVKKVKNMGFKVKFDTNGS
                     RPEIIEKLLKEGLVDYIAMDLKGPLEKYKMITGVEVETEKILKSIDIIKNSKIDYEFR
                     TTVVKKQICIDDFEKIGKIIEGCKKYFLQKFIPSKLVEEDFIKEKTYTDDEFENIKTI
                     MKKYVVFCEVR"
     gene            10595..11767
                     /locus_tag="PLF90_02955"
     CDS             10595..11767
                     /locus_tag="PLF90_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOK56276.1"
                     /translation="MKIAHVITRLVIGGAQENTLFTVEGLIKKGYQVDLISGPTKGPE
                     GSLEKKIIEKKIPLIIIKELVRDINPVYDIIAFLKLFFIFRKNRYNLVHTHSAKAGIL
                     GRISAKLANPKCIVIHTIHGLPFHPYQNKFLNFIYISAEKIAYYFTDHFICVGEIMKE
                     KSLKAGIGKESDYTVIYSGFHVKPYLDCAEKRNEMRKKYGIKKDEKVIGMIGRLFYLK
                     GQNYLIEAFKEVSEKHPEAKLMFVGDGILRNDLENYAKKNKIQDKVIFTGLVPPEKIP
                     EYVSIMDILVHTSLREGLPKAVAQGLAGGKPVIAFDVDGTKEVVINGKTGFIIPPEDI
                     NLLKEKIIYLLENPVISDKMGKEGQELIKQIFPVEKMVDEIEKIYVKFLYNEKRKN"
     gene            11748..12152
                     /locus_tag="PLF90_02960"
     CDS             11748..12152
                     /locus_tag="PLF90_02960"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK56277.1"
                     /translation="MKKEKIDYIIVEASYGSLELISNFIQKKCILSNLSFKKTWELML
                     TVDEICSSIITCKNNDPNIIKVTWENVDNLIKIEIIDDGTSFNPLNVRETDYGLGFQL
                     IKEMIDYCEYKRENGYNVVILKKYKRKCKNKK"
     gene            12149..12919
                     /locus_tag="PLF90_02965"
     CDS             12149..12919
                     /locus_tag="PLF90_02965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOK56278.1"
                     /translation="MKILQKFSLEGKSALVTGGTKGLGKEMAKALAEAGAEVAIVGRE
                     EKAGEDTKKQIIEETSKKIIFIKGDVRKFEDIENFVRITIEEFGKIDILITSAGINIR
                     KNTEDLTVEEAKEIFDTNFFGTWYTCKIVGKYMIERKYGKIVTIGSMLSFVTLPGRSL
                     YSSTKGAIVQLTKTLAVEWAKYNITVNCICPGVFNTPINEKILKDPEIKKFFIEKTPA
                     GRIANPEEIGPLAVFLASDACPFITGSTILIDGGWTCL"
     gene            12923..13666
                     /locus_tag="PLF90_02970"
     CDS             12923..13666
                     /locus_tag="PLF90_02970"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56279.1"
                     /translation="MKKITIFCDGKKWIVDKNLKTFSTHFGNIDIPEKIDGIEKLEFK
                     GRSFYFYQPGISEFLLKIKRKTQIIYPKDVAVITFYSSLKETDNVIETGTGSGSLLIG
                     IMSIVKKGKVITIERNEDFREVAVKNIENYFGKIPENLEFINGDVYDENFKIDIEEEW
                     ADKIFFDLPEPWKAKGLLKYLKKGGILVNYNPQIIQIKKFVEELDGFIDINVFELLER
                     RWLVDEKRARPVDLMRGHTGFIMIGRKAK"
     gene            13663..14964
                     /locus_tag="PLF90_02975"
     CDS             13663..14964
                     /locus_tag="PLF90_02975"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOK56280.1"
                     /translation="MSRKFPVIQVALDFVDEHRALKLAKEVVEGGVEWIEAGTPLIKS
                     CGVEIIRKLRKLFPDRKIIADMKIMDAGRIESEIAFKAGANIVVVMGNASNETIMECI
                     ESSKNYGGEVMVDLIEESANLNRAKEVEKLGANYIGIHISIDKQMTGKISFDFLKQVT
                     KEVKIPVAIAGGLNSENVVDAVKAGASILIIGGAITKSSDAKKAVLDIKKAIETKIPV
                     ETLFYKRVNYENIKEILEKVSTANISDALHRGGWIKGITPILSEKFKIVGKCVTVRTY
                     PGDWAKPVEAIDIAEEGDIIVIDAGGVGPACWGELATNSAIMKKLGGVVIYGGVRDIE
                     EIIKLKFPVFAKVITPTAGEPKGFGEINIPLKIEGQMIFPGDWIVGDMDGVVVIPKEK
                     IVEITNRAMDVLERENRIRKEINEGGTLSSVMDLLKWEKPR"
     gene            14961..15803
                     /locus_tag="PLF90_02980"
     CDS             14961..15803
                     /locus_tag="PLF90_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOK56281.1"
                     /translation="MNRILRKEELAEKVKRIEVEAPLIAKKALPGQFVVLRVDEKGER
                     IPLTIADVDRETGRITLIFQEVGTTTIKLGLLNEGDEILDIVGPLGKPTEIKDYGHVC
                     IIVGGVGAAFVFWMGKAFKEKGNRITTIIGARTKSLIILENEMRQISDNIYISTDDGS
                     YGEKGFNTQILERLLEKGEKFDLVLTAGPIPMMKKIAEITKKYGVRTVASLNPIMIDG
                     TGMCGGCRVTVGGVVKFACVDGPDFDAHSVDFDELLKRTSLYREKEKMSCENFLNSGC
                     IFKK"
     gene            complement(15823..16509)
                     /locus_tag="PLF90_02985"
     CDS             complement(15823..16509)
                     /locus_tag="PLF90_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOK56282.1"
                     /translation="MNYKKVLIFGAHPDDEITMAGTIFKMANSGTKVVVITMTDGCEG
                     YPVLEMKEKIVEIRKKEAKECDKILGISKRIFLDIPDMGLVNNKEILKKCIKIIREEK
                     PDAIFTHGPEDNHRDHINTHFISVEARWHAGEPVSAEFGSPWYTPHLYYYKGITKGLP
                     SVIIDVTDVAEKKYEALITQTSQFTVFRKNKEDFLSEIELIKKTRPKRTEQFWIADKV
                     TIFDFLPRSI"
     gene            complement(16534..17367)
                     /locus_tag="PLF90_02990"
     CDS             complement(16534..17367)
                     /locus_tag="PLF90_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOK56283.1"
                     /translation="MKLGGPIFKKYNNPEEWIKELKKETYSASYCPVDIDTSEEIIAE
                     YRKWAFKEKIIIAEVGAWCNPLSSDIEERKKAIAKCKKGLYLAEKIEAKCCVNISGSR
                     GKKWDGPDKKNLTAETFDMIVETVREIIDEIKPKNTYYTLETMPWMYPDSADSYLKLI
                     NAINRKSFAVHFDPVNLINTPEKYFFNDVLIKDFIKKLGPYIKSCHAKDIIMGDKLTL
                     HFDEIRPGLGQLDYRTLLRELNKLDKNIPLMLEHLPSEEEYRKAKEYIKKISFEEGIK
                     L"
     gene            complement(17375..18502)
                     /locus_tag="PLF90_02995"
     CDS             complement(17375..18502)
                     /locus_tag="PLF90_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOK56284.1"
                     /translation="MNKIILSVIVLVFPLFSQVKTEVKTGGPTVEEVIKYEGPKARIA
                     VARFDVKAPKATWQMGDGVRDMIVDALFKTGKFIVVEKDAIKDLEEEFKFAETGWSKK
                     ELEKGTFEVADIIVVGAITGFEPHAEGTGGGGFVIPTPFGAGIAIKKEEAYIQATIRL
                     IDVRTRRVINSSTIEGKTSKSKVGIAGGAGVSGVILGGGFEKYKNTPTAEAVMIMINN
                     AVKEISRMVPESYYRYGSKETTYKNEPDGFRGIKWLTSVGEINNLKTIKQEGNYKECI
                     KTDDNMQIGKAKLEKIAYSFYKDQLESVTVETKGKDNFENLKSATIERFGEGKNKGEN
                     EWIWEGKITTIILKYKPEEEKGILKMSSEEMKKKIESDTGF"
     gene            complement(18520..>19455)
                     /gene="fmt"
                     /locus_tag="PLF90_03000"
     CDS             complement(18520..>19455)
                     /gene="fmt"
                     /locus_tag="PLF90_03000"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOK56285.1"
                     /translation="FKMKILFFGSDIFGIPSLEMLKRNFEIIGIVTSPDRPKGRGLRI
                     SSTPVKEWAIMNGINIYQPEKFEESFINKIKKISPDLIVLISYGKILPSSILQIPRIC
                     SINVHPSLLPKYRGPAPIEWAIINGETKTGVTIIKMDENIDTGEIIIQKETEILPNDN
                     AFTLKNKLSLLSSDLLYEGIKIIKEGGKTYPQKGEVSYAPKLKKQDGYINWKLDATRI
                     HNLVRGVIIWPTAFTYLKINSRKKLIKIYKTEVGETEGKFGNCGEIIRIEKDYIEVAC
                     GNGTIKIKELQMEGKKRMSTSQFLCGYRNVLKNFC"
BASE COUNT         7254 a         2238 c         3414 g         6549 t
ORIGIN      
        1 atatggtttg ctgatatata caacagtgaa atatacattg attcttttga gggttttaaa
       61 aaagataaag aagataaaat ttttctttat aattttagag agaattcaaa aaaaatagga
      121 agatatgata aatttgaaat tacatttgaa attccttata tttttgaaaa tccttttaaa
      181 ccagatttaa agataaaagg agtttttact tcaccatcag gtaaaattta tgaaactttt
      241 gcttttttct accatgatta tttgagaaca atttatgatg aaggtgagaa gttaattcca
      301 tatggaaaaa gtgagtggaa aataagattt acaccttgtg agacagggag ttatacttac
      361 aagataaagg ttttaaagga taatgaagaa aatgaaatct ataaaggaaa ttttgaagtt
      421 tatgaaagta ataaaaatgg agttgttaac tgggataaaa acgataggta ttacctttct
      481 ttttcaaata gcaaattttt ctatccaata ggtcatactt taaggtcacc tgatgatgaa
      541 agaagccctt ataattatga atttacttat agaaaaggac ttgggacttt cgcttatgat
      601 aaatatttca aaaagatggc tgaaaatgga gaaaattatg caagggtatg gatgggtgta
      661 tggtgggttg gaattgaatg gaattataat tatgcaccac attacaaaaa tcttggtaga
      721 tatagtttag aaaattcatg gaaacttgat tatatttgtg atattgctga aaaatatgga
      781 atttttatgg acttaacttt gataaatcac ggtcagttca gtatcaatcc agatgctgaa
      841 tggtgggata atccttataa tgaaataaat ggtggttttt tgaaatctcc tgatgagttt
      901 tttgtaaatg agaaagcaat agaatatttt aagcagagaa tggattatat tgttgcaaga
      961 tggagttatt ctccaaatat agttttctgg gagttctgga atgaagtgga tttgacaggt
     1021 ttttattctt ctgaaaaagt cagatactgg cataaagtaa tgtatcctta cttaaaatca
     1081 atagaccctt ataaaaaacc aatctcaacc cattattgca gaagagaaga tgaccctatg
     1141 gtatggataa tacctgaact tgaatccctt gtgggtaatg catatcataa tttaattgtg
     1201 gatagtataa gagattatta tgaaaaaaga aaattttatg gaaaaccgat gtttgtaaat
     1261 gagtttggag ttggaaaaga cagatttttt cttgaggata atttacatgg gggtatatgg
     1321 tcttcttcaa tgatgcctat gtttggagtt gctcttttct ggtggtggcc atttattgac
     1381 cattttgacc tttattttca ttataaagca ctttcaaaat tctgggaaaa tgaagacaga
     1441 aggaataagt ttttacaggt atctgatggt aaaataagag ggaaagatgt tgattttgtt
     1501 ggtatccaga ataaagaaga gggatttttt tggatatttg atagaaaaat ttttaacagt
     1561 caacttaaaa gaataaaacc agtaaaaatt gaaaatgcag ttttatattt aaaaggattt
     1621 ttgccgggga agtataaaat tgagttctgg gatacttata aaggagaaat agtaaaagaa
     1681 atgttttatg aaaataaaaa agatattgaa atttcaatta ttccttttga aaaagacatc
     1741 gctttaaaaa taaagagaca aaaatgagaa atatttattt attcttttta attctacagt
     1801 tttttgtttt ttctgaatac agatatttgt tgaatttatc ctgtgaaaat aaaaaactcc
     1861 ttgaaacatt taaaattgcc tcctttaatt ttatttcagg aaaggaaaaa tatgatgata
     1921 ttaaaattat tgatgaaaat ggaaaagaat gtgaaatctc tggagttaat tttttaaaag
     1981 atgagatgga aatttttttt aaaccaacag attcaattaa ttataaggtt tttatcggtt
     2041 acagtgaaaa aaacagatta tacaaagaat ttaaaaaagg aaatattttg atagatgatt
     2101 atatttttcc agattcaatt aaatccggtg tatggttatg gactgaaaaa tcactctcag
     2161 gaatttattc tcataccggt actgctggtt tttctttcca cagagtcagt tttccaaaac
     2221 aattttctat aggttcagat gataaaatta tttcttatct tttcatagaa gaagaaaatg
     2281 ctccagaaga gataattata gaagttattt caagatatag aagacactat tatttttctt
     2341 tcggtaagga tgtaataaat atgaaaggta tgaataaaga aaaaattgga gaacttccag
     2401 aaataggaaa atggacaaaa attgaaatac ctcttttaaa aataagagaa aaaaacatag
     2461 aaggaattgg tttttataac gataaaggaa gggtttactg ggacatgact tctataaatg
     2521 aaatacctgt taagatgaaa attagaaaag ttgaaaattt aaagtcaccc aatactccct
     2581 attttaatta tgagattgaa ggtccattta ctattgataa taaaaaaata acaattttga
     2641 aacttgatac atcctgtttt ttatctgata aatatttgtg ggaaattgat ggaaaagtat
     2701 ataccggtga aaaaattgtt gtaaagttag aaggagaaaa aaaagagata aatataaaac
     2761 taaaaacaga gaaaaataaa atatctggag agtttatcca taaaataagt ttaggagatg
     2821 gaaaagaaat aaagaccatt tttaatattc ttccttttga aaattttata tatgaggatg
     2881 aaaaacttta tataccaatt agagttgaaa atatatctga cattccttta gtattaactc
     2941 ttgcatctca aaacaataaa aatgaatttt attttctccc aggaaaagaa aatgggaaaa
     3001 caatgacttt gtttttaaat cctgaaaatg aaataaaaat tgggatttat ttttatgatg
     3061 cgaaactcgc tgaaaaaact ataaatgtgg tggatttaaa tgaaaaggat ttttttgttg
     3121 atggtgtttt tttaaagaaa aataattctt accttatatt taaaattcct gaatatacaa
     3181 atataaaaaa aactactgga ttttcaaaaa taatttttat tggagatttt ccagacgagt
     3241 taattcaatt ttttaaagaa aaaggaatag aagttttaaa tatatccgag gtaaaatatg
     3301 taaattatct ttttaatgaa tttaagttta ttcaggaaat tcaggataaa ataaacagcg
     3361 attcttttgt tgttttattt ccatttataa attctctttt aagaaggcat agaataaatg
     3421 aatgggaaaa ggtttatgat gcaattttat atcttttatc acaaaaaaat tcaaatataa
     3481 ttgtggtttc tccatttcct tcatatcctt atttagaagt tcttcaacca tatagagaaa
     3541 aaattaagaa gatttctgaa aaaagaaatt tttattttct tgacttatat aaaatctttt
     3601 ctgaaattga aaatggtgaa agatttttta aaatgaatga gtatgtttat aaaaatttac
     3661 ctgataaaga gggttttgaa ataattataa atgagattaa aaaaattatt gaaaatgaaa
     3721 aaaattttaa taatcagaga gggagcactc ggtgatttaa ttttaacatt tcctgttttt
     3781 ttcagtttta aaaaaaatgg gtacagagtt tttgcaggag gaaagggtat ttataaaaat
     3841 tttattgaaa agtatggaaa tgttgagaaa tttattccac ttgattcaag tgaatatctt
     3901 tttttctttg aaagagagaa taccaagttg aaaggttttc ttaaagattt tgatattatt
     3961 attgcctaca cagatgaaaa agaaataata ggagaaaatc taaagaaaaa ttttatgaat
     4021 aaaataattt tccaccctgt taaaaaggag aaattgaata tccacattac agattattta
     4081 atgaagccag ttaaggaaat atttaataaa aatttatatt tttctataaa tttaaattta
     4141 aaaaataaag gagaattttt tttaattcat ccgggaagcg gaagtaaaga taaaaactgg
     4201 gggaaagaga agtttttgaa actttcagaa aatctgaaaa atgtaaaatt acttcttgga
     4261 cctgcagaag aaaatgaact ttcattctgg aagagaaatt ttaaaggaga aatttttttt
     4321 aatcttgatt tagaagatat tattaaaatt gccgaaagaa ctattgctta tattggaaat
     4381 gattccggaa ttactcacct tttcagtatt acaggagtaa aaacaattgc tatttttgga
     4441 cctacttcac cttttatttg gggtccgaga ggaaaaaatg ttaaaattgt ttacaaaaat
     4501 ttggagtgca gtccttgtgt atatgaaaaa atgaaaaatt gtacagataa aaaatgtttg
     4561 gaagagataa aagttgaaga tattctttct tttatttcgg acggttaaaa ttagatgtta
     4621 aaaggatttg ataaatttaa aggaggatgg tatatggaaa aggcagttat ttttgatgga
     4681 agtaaaggta aactatttgg ggttattcat attccagaaa aggagaaatc acccggaatt
     4741 gtactttttc atggttttac tggaaataaa tgtgaaagtc actttatatt tacaaaactc
     4801 gcaagaaaat tatgtaggga gggtttttct gttttgagat ttgattttta tggttcaggt
     4861 gatagtgaag ggaattttta tgaaatgact cttgaaacag aaatgaaaga cggagaaaaa
     4921 gcattagaat tttttaaaac atttgatttt gttgacagag aaaaaattgg agtttgtggt
     4981 ctttcaatgg gtgctgttac tgcagtttat gttgttcata aatttaagga agttaaggga
     5041 ttatgtttat ggtctcccct tgcatttcca aaaattatac agaagaaaat tttaacaaaa
     5101 aaaataagag aaaaaataga aaaatatggc aagtgttata tacctggaat gggtcattat
     5161 atcggaaagg gatttattga atctcttgga aaaattgata tgaaagaata tgtagaaaat
     5221 tataggggaa atgtttttat catacataca aaagatgacc tttctcttga cttgaaaaat
     5281 gcactttttt attttgaaaa atttcataaa aacgcgagta atcttaaaat gcttgtgctt
     5341 gataaaggag ggcatgtttt taccacagaa gaaagcgaaa atacagttat agatgagaca
     5401 acagaattct ttaaagaatg ttttaaaaag gagtaaaaat gaaaaaagaa attaaatata
     5461 ttgatgtaat ggaagaaaca attgaagcat taaaggaagg cagagttctt gtttgttctg
     5521 tggataaaaa taataaaccg aatgtgatgg cgattggatg gggaagtatt ggtattatct
     5581 ggggtgaacc tatttttttt atttttataa gaccttcaag atatacattt aatctgattg
     5641 aaaaaacaga ggattttact gttaatatct ttgaagagaa atataaggaa atagttaact
     5701 ggtgtggcac agtttcagga agagaatatg ataaatttaa agaaaaaaat ctgacacctt
     5761 taaaatcaaa atatgtaaaa tctcctgttg ttaaagaaag tattataagt tttgaatgta
     5821 aagttattgc aaggatggat gttaaaccgg aaatgattga taaagaaata aaagaaagaa
     5881 tgtataaatc aggtgattat catcgcgtat atttaggtaa aattcttgcc tcttataaaa
     5941 atttcacttg acattataga aactttttgg tatttttttc atctaataat aaaaaaggag
     6001 gaagttatgc ctacttatga atatgaatgc aaaaattgtg gttataaatt tgaaaaattt
     6061 caaaaaatga ctgatgaacc tttaaatata tgtcctgaat gtaaaaaaga aagtttaaga
     6121 agattagttg gagttggcgg tggtgttatt tttaaaggac ctggatttta cacaacagaa
     6181 tatagaagtg aagattataa aagaaaagaa agagaggaaa agggattgag ttctacatct
     6241 tcaggttcct gttcctcctg tagtgcttct tcctgttcaa cctgtaaaaa gtaaataagg
     6301 ttcatttttt cccgctattt ttattttgac ttgacaaaat ttgtgtatat gttaaatttt
     6361 aaaaaaatac aatatgttgt tattttaaac ttcttgatgt actatatata gaaaatgaaa
     6421 tgtccttact gcggttattt aaaagacaaa gtggttgatt caagagaaag agaagatggt
     6481 ttgttaataa gaagaaggag aatatgtctg aagtgtaaaa gaagatttac cacttatgaa
     6541 gagatagatt taaaaccatt aattgttgtt aagaaagatg gtagaagaga aaggtttaac
     6601 agagaaaaat tgataatcgg gatacagaag gcatgtgaga aaagacccat aagtacagat
     6661 aaaataaatg agattgttga agatattgaa aaagaattga gacagaaata tgaaaatgaa
     6721 gttacctcca aagaaatagg aaaaagtgtt ataaaaaaat tgaaaaaact tgataaagtt
     6781 gcatatatta gatttgcttc tgtttatcag gaatttgaag atattgaaca gtttttaaaa
     6841 gaaatagaaa aaataggggg aaaaaatgtt taaaaagatt gtaaaaagag acggaagaat
     6901 tgttgatttt gattcaacta aaataacaaa tgctattctt aaagcaggtc aggcaaccgg
     6961 tgaatttgga atagatgttg ctaaaaaatt gacagtaaaa gttcttgacc ttgcactgga
     7021 aataataaaa gataaaattc caacagttga ggaaatacag gacattgtag aagaggttct
     7081 tttactttct ccctataaaa agacagcaaa agcatatata atttacagag aacaacacgc
     7141 aagaataagg gaaataacaa caaaagcaag tttagacctt gtggaccagt atttaaatca
     7201 aattgattgg agggttaaag agaacagtaa tatgagtttt tctcttcagg gtttaaataa
     7261 ttatgtttct tccgaagtaa caaaaacata ctggttaaat aatatatatc ctcctgaaat
     7321 aaggaaagca tatattgatg gtgattttca tattcatgac cttggttctt tatctgttta
     7381 ttgtgttgga tgggatttac aggatttact tttaacaggg tttaaaggtg cagaaggaaa
     7441 agtggaaagc agtcctgcaa aacattttgg ttcaatttta gggcagattg ttaatttctt
     7501 ttatactctt cagggagaaa gtgcaggtgc tcaggcattt tcaaatcttg atacccttct
     7561 tgctcctttt gtaagatatg atggtttaag ttataaagag gtaaaacagg cggttcagga
     7621 gtttttattt aatttgaatg taccaacaag aacaggtttt caatgtcttt cagaagatac
     7681 agaaatttta actcctgacg gctggaaaaa atatgaccag gttaaaaaag gagatacaat
     7741 ttatacattt aatataaaaa acggaataat agaaaaaaaa gaagtaaaac atgtttttgt
     7801 aagagaatat gaaggagtaa tgtacaatct aaaaaataga aatcaagacc agttaatatc
     7861 tccagggcac agagttgtaa gaagaatttt taatacagat agatttgctc ttgaaccaat
     7921 tgaaaaagta attgaattaa tgtctcctgt tgtaattcct gtaaaaggag agaatataaa
     7981 tgaggaatat gatatagagg attcaaaaat aaagttgctt gcatggattc tttctgaagg
     8041 gagtatagaa aagcagggaa gtcatagagt aagtatatat caatcagaag aaaaacatcc
     8101 agaaaattat aatgaaataa ttgacttact aaaaataaat gaacttgaat attctataag
     8161 acctcagagt tccctgggta tatgtactca tataagactt aagtcaaaac caagcaaagt
     8221 tattcataat ttattaggtt ctgaaaagaa acaattccct tcttttcttt taaagttaag
     8281 taaaaaacag gcaaaattat ttattgatac atatataaaa ggtaatggat gggaagaaaa
     8341 atttagaaaa agaattactg ttactgataa agaagcaatg gatattatta caggagtggc
     8401 tgtacttgca ggatataatt ttaatgttaa ggagagagaa gttgggggta tttcaaaaaa
     8461 aactcagtat attatcacac ttacagaaac acagtatgat tatattcagg agataaaacc
     8521 ggttgaatat aaagggataa tctggagtgt taatactgaa aatgaaactg tcatagcaag
     8581 aagaaatgga cagattttta taactgggaa tacccctttc acaaatctta cttttgactt
     8641 aaaagtttca gaagtttata aagaccagcc agttattatt ggaggaaaga ttcaggataa
     8701 aacttatggt gaatttcaga aagagatgga tatgataaat aaggcatttt ttgaagttat
     8761 gcttgaagga gatgcaaagg gaagggtatt tacatttcct atcccaacat ataacataac
     8821 aaaggatttt gactggaaaa atgaaacact tgatttactc tgggaaatga ctgggaaata
     8881 tggtattcct tatttttcca attttataaa ttctgatatg aaaccggatg atgtgagaag
     8941 tatgtgctgc agattgagat tacagacaga tgagttaaag aaaagaggag ggggattatt
     9001 tggagcaaat cctttaacag gaagtatagg agttgttaca attaatttac caagaattgg
     9061 atatttatca aaaacaaaag aggaatttaa aagtagattg aatgatttga tggaagttgc
     9121 aaaagaaagt ttagaaataa aaagaaaagt tcttgaaaga tttacagatt ctggacttta
     9181 tccatattca agattttatt tgagaaatgt gaaagaaaga tttgggaaat actgggtaaa
     9241 ccatttttca accataggaa ttattggtat gaatgaagca tgtttaaatc tttttggtaa
     9301 agatatagga acagaggaag gaattaattt tgcaattgag ataatggaat ttatgagaga
     9361 aaaattgatt aaatttcagg aaagtacagg taatttttat aatcttgagg caactccagc
     9421 agaagggaca agttatcgtc ttgcaagaat tgataaaaat aaatatccag atataattgt
     9481 tgcaaatgag aaggaatgta gaaacggagc ggaaccattt tatacaaatt caacacactt
     9541 acctgtaaat tatactgatg atatatttga ggtacttgaa aaacaggata aacttcaatc
     9601 gctttataca ggtgggactg ttatacatat ttttgtaggt gaaaaaataa attcaaaaga
     9661 aggaattaaa aaacttgtga aaactatatg cgaaaacttt actttacctt attttacaat
     9721 aactcctaca ttcagtgtct gtcctgaaga tggttattta aatggagagg tatataactg
     9781 tccaaaatgt ggtaaaacaa cagaaattta ttcaagaatt gttggttatt taaggcctgt
     9841 gagtcaatgg aataagggta aatatgaaga gtttaaaata agaaaaacat ttgtccttaa
     9901 atgaaaatag gtgcaataca gaaaacgtct ttaattgaat ttcctaatat tttaagttgt
     9961 attattttta ctcagggatg taattttaga tgtccttact gtcataatcc tgaacttgtc
    10021 ttacctgaaa aatttggaga aattatacct gaagaggaaa tttttgattt tttgaacaga
    10081 agaaaaaaat atcttgaagg tgtttgtatt acaggtggag aaccatcgat tcagccggat
    10141 ataattgatt ttgttaagaa agtgaaaaat atgggtttta aagtgaagtt tgatacaaat
    10201 ggttcaagac cggaaattat agaaaaactt ttaaaagaag gattagttga ttatattgca
    10261 atggatttga aagggccatt agaaaaatat aaaatgatta caggagtgga agtagaaaca
    10321 gagaagatat taaagtcaat agatataata aaaaattcaa aaatagatta tgaattcaga
    10381 actacagtgg ttaaaaaaca aatttgtatt gatgattttg aaaaaatagg taaaattatt
    10441 gaaggttgta aaaaatattt tttacagaaa tttattccat ctaaactggt tgaagaagat
    10501 tttattaaag aaaagacata tacagatgat gaatttgaaa atataaaaac cataatgaaa
    10561 aaatatgtag ttttttgtga agtgagataa tgatatgaaa attgcacatg ttataacccg
    10621 tcttgttatt ggtggagcac aggaaaatac tttgtttact gttgaaggtt taataaaaaa
    10681 gggatatcaa gttgatttaa taagtggtcc cactaaagga cctgaagggt ctcttgaaaa
    10741 aaaaattatt gaaaaaaaaa ttccacttat aataataaag gaacttgtaa gagatattaa
    10801 tcctgtttat gatataattg cttttttaaa actatttttt atcttcagaa agaatagata
    10861 taatttggtt catactcaca gtgcaaaagc aggaatactc ggaaggattt ctgcaaaact
    10921 tgccaatcca aaatgcatag taattcatac aattcatgga ttaccatttc atccatatca
    10981 aaataaattt ttaaatttta tttatatctc tgccgaaaag attgcatatt atttcactga
    11041 ccattttata tgtgttggtg aaataatgaa agaaaaatca cttaaagcag gtataggtaa
    11101 agaaagtgat tatactgtga tatacagtgg atttcatgtt aaaccatatc ttgattgtgc
    11161 agaaaaaagg aatgaaatga gaaaaaaata tggaataaaa aaagatgaaa aagttatcgg
    11221 tatgattgga agattgtttt atctaaaagg acagaattat ttaatagaag catttaaaga
    11281 agtatctgaa aaacatccag aagcaaaact tatgtttgtt ggagatggta ttttaagaaa
    11341 tgatttggaa aactatgcaa aaaaaaataa aatacaggat aaggttattt ttacaggact
    11401 tgttccacca gaaaaaattc ctgaatatgt aagtattatg gatatacttg ttcatacaag
    11461 tttaagagaa ggtcttccaa aagcagttgc tcagggactt gcaggaggaa agccggttat
    11521 tgcctttgat gtagatggaa caaaggaagt tgtgataaat ggaaaaactg gttttattat
    11581 tccaccagaa gatataaatt tgttaaaaga aaaaattatt tatttacttg aaaatcctgt
    11641 aatttctgat aaaatgggga aagaaggaca ggaattaata aaacaaatat ttccagttga
    11701 aaaaatggtt gacgagattg aaaaaattta tgtaaaattt ttatataatg aaaaaagaaa
    11761 aaattgatta tataattgtt gaggcaagtt acggaagttt agaattgatt tcaaatttta
    11821 ttcagaaaaa atgtattctt tcaaatctaa gtttcaagaa aacatgggaa ttgatgttaa
    11881 cagttgatga aatctgtagt tcaataataa cctgtaaaaa taatgaccca aatattatta
    11941 aagttacatg ggagaatgtg gataatttaa taaaaataga aataatagat gatggaactt
    12001 cttttaatcc tttaaatgta agggaaacag attatggact tggatttcaa cttataaaag
    12061 aaatgattga ttactgtgaa tataaaagag aaaatggtta taatgtggtg attctgaaaa
    12121 aatataaaag gaaatgcaag aacaaaaaat gaagatttta caaaagtttt cattagaagg
    12181 gaaatcggca ctggtaacag gtggaacaaa agggcttgga aaggaaatgg caaaggcact
    12241 tgcagaagca ggtgcagaag tcgctattgt tgggagagaa gaaaaagcag gagaagacac
    12301 aaaaaaacaa attattgagg aaacaagcaa aaagataata tttataaaag gagatgtaag
    12361 aaaatttgaa gatattgaga attttgtgag aataactatt gaagaattcg ggaaaataga
    12421 tatactaata acttcagctg gaataaatat aagaaaaaat accgaggatt taacagttga
    12481 agaagcaaaa gagatttttg atacaaattt ttttgggacc tggtatacat gtaaaatagt
    12541 tggaaagtat atgatagaaa gaaaatatgg gaaaattgtt acaattggtt caatgttaag
    12601 tttcgtaacg cttccgggaa gaagtcttta ttcttctact aaaggagcaa ttgttcaact
    12661 tacaaaaaca cttgctgttg aatgggctaa atataatata accgtgaatt gtatatgtcc
    12721 tggagttttt aacacaccca taaatgagaa aattcttaaa gaccctgaaa ttaaaaaatt
    12781 ttttattgaa aaaacacctg caggaagaat agcaaatcca gaagaaatag gacctcttgc
    12841 tgtttttctt gcctctgatg cctgtccttt tataaccgga agtacaattt taatagatgg
    12901 aggatggacc tgtctgtaaa aaatgaaaaa aattactatt ttctgtgatg gtaaaaaatg
    12961 gatagttgat aaaaatttaa aaacattttc aactcatttt ggaaatattg atatccctga
    13021 aaaaatagac ggtattgaaa aacttgaatt taaaggtaga agtttttatt tttaccaacc
    13081 tggcatttca gaatttcttc ttaaaataaa aaggaagact cagataatat atcctaagga
    13141 tgttgctgtg attactttct attccagttt aaaagaaaca gataatgtta ttgaaactgg
    13201 gacaggttct ggttctttat tgataggtat tatgtcaata gtaaaaaagg gaaaagttat
    13261 aactattgaa aggaatgagg atttcagaga agttgctgta aaaaatattg aaaattactt
    13321 tggtaaaata ccagaaaatc ttgaatttat taatggcgat gtttatgatg aaaattttaa
    13381 aattgatatt gaagaagaat gggctgataa gatttttttt gatttaccag aaccatggaa
    13441 agcaaaagga cttctaaaat acttgaaaaa aggtggaatt ctggtaaact ataatccaca
    13501 aattatacag ataaaaaaat ttgttgaaga actggatggt ttcattgata taaatgtgtt
    13561 tgaactcctt gaaagaaggt ggcttgttga tgaaaaaaga gcaagaccgg ttgatttaat
    13621 gagagggcat acaggtttta ttatgatagg taggaaggca aaatgagcag aaaatttcct
    13681 gtaattcagg ttgcacttga ttttgttgac gaacacaggg cattaaaact tgcaaaagaa
    13741 gtagttgaag gtggtgttga atggattgaa gcaggaactc ctttaataaa atcatgtgga
    13801 gttgagatta taagaaaact cagaaaactt tttcctgaca gaaaaataat cgctgatatg
    13861 aaaataatgg atgccggtag aattgagagt gaaattgcat ttaaagcagg agcaaatata
    13921 gttgttgtaa tgggaaatgc atcaaatgaa acaataatgg agtgcataga gtcctcaaaa
    13981 aattatggtg gagaggtaat ggttgacctt atagaggaat cagcaaattt aaacagggca
    14041 aaagaggttg aaaaacttgg tgcaaattat attggcattc atatttcaat agataaacag
    14101 atgacaggga aaatttcttt tgattttctt aaacaagtta caaaagaagt taaaattcct
    14161 gttgcgatag cagggggatt gaattctgaa aatgttgttg atgcggttaa agcaggtgcc
    14221 tcaattttaa taataggtgg tgcaataaca aaatcatctg atgcaaaaaa agcggtttta
    14281 gatataaaaa aggcaattga gacgaaaata cctgttgaaa cattattcta taaaagggtt
    14341 aattatgaaa atataaagga gattctggaa aaagtttcaa cagcaaatat ttcggatgct
    14401 ttacacagag gaggatggat taaaggtata actcctattt tgagtgaaaa gtttaaaatt
    14461 gttgggaagt gtgtaactgt tagaacatat ccaggggatt gggccaaacc agttgaagca
    14521 atagatattg ctgaagaagg agatataatt gttatagatg caggtggagt tggtcctgct
    14581 tgctggggtg aacttgcaac aaatagcgca attatgaaaa aattaggagg ggttgttatt
    14641 tatggaggag ttagagatat tgaagaaata ataaaactta agtttcctgt atttgcaaaa
    14701 gttataactc ctactgcggg tgaaccaaaa ggttttggag aaataaatat tcctttaaaa
    14761 atagaaggac agatgatttt tccaggtgac tggattgtag gtgatatgga tggtgtggtt
    14821 gtaataccaa aagaaaaaat tgttgaaatt acaaatagag caatggatgt tcttgaaaga
    14881 gaaaacagaa taagaaaaga aataaatgaa ggtgggactt taagcagtgt aatggattta
    14941 cttaaatggg agaaaccaag atgaatagaa tattaaggaa agaagaactt gctgaaaaag
    15001 taaaaagaat agaagttgag gcaccgttga ttgcaaaaaa agcattacca gggcagtttg
    15061 tggttttaag agtagacgag aaaggagaaa gaatacctct gaccattgca gacgttgata
    15121 gagaaacagg aagaattaca ttgatttttc aagaagttgg gactactact attaaacttg
    15181 gacttttaaa tgaaggagat gaaatacttg atattgtagg acctcttgga aaaccaactg
    15241 aaataaaaga ttatggtcat gtttgcatta tagttggtgg agtaggggct gcttttgttt
    15301 tctggatggg taaagcattc aaagaaaaag gtaatagaat aactactata ataggagcga
    15361 gaactaaaag tttgataata cttgaaaatg aaatgagaca gataagtgat aatatttaca
    15421 tttcaacaga tgatgggagt tatggagaaa agggatttaa tacacaaatt cttgagagat
    15481 tacttgaaaa aggagaaaaa tttgatttgg ttttaacagc gggtcctata ccgatgatga
    15541 agaaaatagc agaaattacg aaaaaatatg gtgttagaac tgtggcaagt ttaaatccaa
    15601 taatgataga tggaacaggt atgtgtggtg gatgcagggt tacagtagga ggagttgtta
    15661 aatttgcctg tgttgatggt cctgattttg atgcccattc tgttgatttt gatgaacttt
    15721 taaaaagaac ctcactttac agagaaaagg aaaaaatgtc ttgtgaaaac tttttaaatt
    15781 caggatgtat ctttaaaaaa tagttataga acttgctttt tattaaatac ttcttggtaa
    15841 aaaatcaaaa attgttactt tatctgcaat ccagaattgt tcagttcttt ttggtcttgt
    15901 ctttttaatc agttcaattt cagataaaaa atcttcctta ttttttctga aaactgtaaa
    15961 ctgtgatgtc tgagtgatta atgcttcgta ctttttttct gccacatccg ttacatcaat
    16021 aataactgaa ggcaatccct ttgtaattcc tttgtaataa tataaatgag gtgtatacca
    16081 gggacttcca aattcagcag agacaggttc tccagcgtgc catctcgctt ccactgaaat
    16141 aaaatgtgta ttaatatggt ctctgtggtt atcttccggt ccatgagtaa atattgcgtc
    16201 aggtttttct tctcttataa tctttataca tttttttaat atttctttat tatttacaag
    16261 tcccatatca ggtatatcaa gaaatattcg tttagatatt ccgagaattt tatcacattc
    16321 ttttgcttcc ttttttctga tttctacgat tttttctttc atttctaaaa ctggatatcc
    16381 ttcacaacca tctgtcattg ttattactac aacttttgta cctgaatttg ccattttaaa
    16441 aattgttcca gccattgtta tttcatcatc tggatgtgct ccaaaaataa gtactttttt
    16501 gtaattcata attaaccctc cttctaaaaa aatctatagt tttattcctt cttcaaaaga
    16561 tatttttttt atatattctt ttgcttttct atattcttct tcagaaggta gatgttcaag
    16621 catcaaagga atatttttgt ctaatttatt aagttctctt aaaagtgttc tgtaatcaag
    16681 ttgtccaagt cccggtctta tttcatcaaa atgtaatgtt agtttgtcac ccattattat
    16741 atcttttgca tggcatgatt ttatatatgg tccgagtttt ttaataaaat cctttataag
    16801 aacatcattg aaaaaatatt tttctggagt atttattaaa ttcacagggt caaaatgaac
    16861 tgcaaatgat tttctgttta ttgcattaat aagttttaag taactatcgg cagaatcagg
    16921 atacatccac ggcatagttt ccagtgtata atatgtattt ttcggtttga tttcatctat
    16981 aatttctcta actgtttcta ctatcatatc aaatgtttct gcagtcaaat ttttcttatc
    17041 aggtccatcc catttttttc ctcttgaacc agaaatattt acacaacatt ttgcttcaat
    17101 tttttctgcc agatataagc cctttttaca ctttgctatt gctttttttc tttcttcaat
    17161 atcagaactt aaaggattac accatgctcc gacttctgca ataataattt tttctttgaa
    17221 tgcccattta cgatattcag caataatttc ttcggatgta tctatatcaa caggacaata
    17281 agaagcagaa taagtttctt tttttaattc tttaatccat tcttctggat tattatattt
    17341 tttaaaaata ggaccaccaa gtttcatttt taaattaaaa tccagtatct gattcaattt
    17401 tctttttcat ctcttctgaa gacattttta aaattccttt ttcttcttca ggtttatatt
    17461 ttaaaattat tgttgtaatt tttccctccc atatccattc attttctcct ttattttttc
    17521 cttcaccaaa tctttctatt gtggctgact ttaaattttc aaaattatct tttccttttg
    17581 tttcaactgt tacactttca agttggtctt tataaaatga gtatgcaatt ttctcaagtt
    17641 ttgctttccc tatttgcatg ttatcgtctg tttttatgca ttctttataa ttgccttcct
    17701 gtttaattgt ttttaaatta tttatctctc ctacagaagt taaccatttt atacctctga
    17761 atccatcagg ttcattctta taagtggttt ctttacttcc atatctataa taactttctg
    17821 gaaccattct tgatatttct tttacagcat tattaatcat aatcataact gcttctgctg
    17881 ttggtgtatt tttatatttt tcaaatccac ctccaagtat aactccacta actcctgctc
    17941 caccggcaat tccaactttt gattttgatg ttttaccttc aatagttgaa gaatttataa
    18001 cccttcttgt tcgtacatct atcaatctta ttgtcgcctg aatatacgct tcttcttttt
    18061 taattgcaat ccctgctcca aaaggtgttg gtattacaaa tccacctcct cctgttcctt
    18121 ctgcatgtgg ttcaaaacct gttatagctc ctactacaat tatatctgca acttcaaatg
    18181 ttcccttttc aagttctttt ttactccatc ctgtttctgc aaatttaaat tcttcttcaa
    18241 ggtcttttat tgcatctttt tccacaacta taaatttacc tgttttaaaa agagcatcaa
    18301 caatcatatc tctaactcca tcacccattt gccatgttgc ttttggtgct tttacatcaa
    18361 atcttgctac tgctattctt gctttaggtc cttcatattt aattacttct tctactgtgg
    18421 gtcctccagt ttttacttct gttttaacct gagaaaataa aggaaatact aaaactatca
    18481 cagataagat tattttattc atattccctc cctttttatt taacagaaat tttttaaaac
    18541 atttctgtat ccacataaaa attgagaagt actcattctt tttttccctt ccatctgcaa
    18601 ttcttttatt tttatagttc catttccaca tgcaacctct atataatcct tttcaattct
    18661 tataatttct ccacaatttc caaatttgcc ttctgtttct cctacttctg tcttatatat
    18721 ttttatgagt ttttttcttg aattaatttt taaatatgtg aaagcagttg gccaaattat
    18781 aacacctctg acaagattat gtattctggt tgcgtctaat ttccagttaa tatatccatc
    18841 ttgctttttt aatttaggag cataagaaac ttcgcctttt tgaggatatg tttttccacc
    18901 ttctttaatt atttttattc cttcatataa aagatcagaa gaaagcaacg ataatttatt
    18961 tttcaaagta aaagcattat cattaggcaa tatttctgtt tctttctgga ttattatttc
    19021 acctgtatca atattttcat ccattttaat tattgttaca cctgtttttg tttctccatt
    19081 tattatagcc cattcaattg gagcaggccc tctatatttt ggtagaagag aaggatgaac
    19141 atttatagag caaatcctgg gtatctgtaa aattgaagaa ggaagtattt tcccatacga
    19201 aatcaaaaca attaggtcgg gtgaaatttt ttttatctta ttgataaaac tctcttcaaa
    19261 tttttcaggt tgatatatat taattccatt cataatagcc cattctttta ctggagttga
    19321 agaaattctt aatcctcttc cttttggtct gtctggtgag gtcacaatac caataatttc
    19381 aaaatttctt ttaagcattt caagagaagg aataccaaaa atatcgcttc caaaaaataa
    19441 tattttcatt ttaaa
//
LOCUS       DAOSAT010000021        19404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_1975_length_19404_cov_4.628353, whole genome shotgun sequence.
ACCESSION   DAOSAT010000021 DAOSAT010000000
VERSION     DAOSAT010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1975_length_19404_cov_4.628353"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLF90_03005"
     CDS             <1..437
                     /locus_tag="PLF90_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOK56286.1"
                     /translation="SIGLNYAPPNRNLLSLREVLLIKGFDKEKVYGNKEKKGLFDFIT
                     IYSDDKININTCKSELLNSMGFTNEDISKIMEERENRPINETILLNINRNVFLKNKNL
                     IKFKSNYFHISLKVKDEKGKERNVEGILKKDKTIELIKKGIL"
     gene            434..1657
                     /locus_tag="PLF90_03010"
     CDS             434..1657
                     /locus_tag="PLF90_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56287.1"
                     /translation="MKIFLYLKGEKVYYFKNNERGEIPFENLENFIKENKQNFFYLIF
                     SKLNIYFRKVEFEFRDKKKISLVLNQEIEGKFPTSIDKLYFHFKFFYPEKDKTTIGIF
                     AVEKEKIEYLKEIFKKNKTKYKFLIDSILIHQFFRNNLKEYDFIELYTEKDYLLINLI
                     ENSTISGVYSYFSNNIKESILEVLPSLFVTKKLPVYFIGDRKIYEEIKMDGLHFFSET
                     NFFNILNELKKLEVVNFKDILIRKKILNFEYLSYFIILLVSTFLFFTPHFKITEKEKK
                     LDEINKKMENIFKSLFPETEKIVNPLIQIKEKFIQDKSYSKIPVYEISVIKTLEEITL
                     FFPENINGEVEEVSINDKNINLTGIVDNLKNLDNLKENIRNSKFFKNFEINSVSFTKE
                     NKIRFNLLLRTGTNG"
     gene            1650..2159
                     /locus_tag="PLF90_03015"
     CDS             1650..2159
                     /locus_tag="PLF90_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56288.1"
                     /translation="MDRKIILTFVIFIIIFEYQYIYLPNKKKIKNLDSIILKKEKEYK
                     EFIELCEKYKKGEVEKKDTTVNVVSDKFSFFSYLNDLIENSSIKTNFGDIKILPREEI
                     DEYIMEKIQIDINLITLEQLLSILNKIEKTKGIYISQFEMKRDKNKPYLLNVSMIITC
                     LKNRITDNF"
     gene            2162..4573
                     /locus_tag="PLF90_03020"
     CDS             2162..4573
                     /locus_tag="PLF90_03020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOK56289.1"
                     /translation="MEDIYLIDGTCLIYRSFYALPKLKTSTGIITNAIYGFTTTLLKI
                     LKEKKPEYMAIFFDLQVPTFRHITFEKYKEKRKPMPDELSGQIPKIKEIITYLGIKYY
                     EKEGYEADDLIASSIEKLKSDDRVFFIISSDKDILQLIDKNVFVINPIDYSILDEKSV
                     INKYGFRPEKIVDFIGLAGDTSDNIPGIPGIGEKTAISLLSKFNSIEEIYNNLEKIDS
                     KNLRDKLEKNREIAFLSKKLSTLNKNFFDKITLEEIKINSPDLPRLYEIFEYFEFTKL
                     KEIIKEIYPDVENIESIENFIGFSTGEIINYTEILRYPEKYKKILECDDIKKIGFDLK
                     SKIVDLINKGIKLKNIDFDFSIAKHLTGKAIYNRNLFELKKEYENLLIGFNMYKLFEE
                     VEMPLIYVLVWMEITGIKVDIEYLKNLNEKFNIEINEIIDKIYSLSGEVFNLNSPNQV
                     GEILFKKLKLPVIKKTKTAYATDINVLKQLSPLHPLPKLLLEYRELYKIKSTYIEGLL
                     NFINQKTGRIHPTYSQISTTTGRLTCSNPNLQNLPVKSQRGGLIRKAFCAEKNNFLLS
                     FDYSQIELRVLAHFSEDPVLIDAFEKDRDIHSETAEIILTSDSLFSPLNFGELTKEEK
                     RRIAKTINFGIIYGISPYGLSQELGISVEESSIFIQKYFEKFKKVKQYTENIIKKAED
                     NGYVETLLKRRRYIPELKSQNKNEREFGKRVAINMPIQGSASEIIKLSMNKIYQKFKE
                     ENLKSNLILQIHDELLFEVLPEEEKKVIEIVKEIMKNAIILKVPIKVDVKKGKNFLEM
                     EEIND"
     gene            4566..5528
                     /locus_tag="PLF90_03025"
     CDS             4566..5528
                     /locus_tag="PLF90_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK56290.1"
                     /translation="MIDIRMYLCKNFYMRKFQIFLMFFLIVSLSYSYWEWTPQTKKWI
                     NPKYAVKETPEEQFKYAEELRKNGKIEASIREHKKLLKHYSKSEYAPSSCFILGEIYR
                     EKGDIKKAFDYYQKIIDEYPSSPLVLSSIKIQSEIAEKKLESKKGLFRGLFSRAEEKA
                     QFMNKVIENSPYDLEAVDRMFKLADFYFDLKEYDKSIEVLEKIIKNFPETSNAERAKF
                     LKIKYLLNLIPEVSLDIDTIEDIKDQINEFLIEYPNSRFENEIKKIEIMLDEKEAEKY
                     YQIARYYERAGKKKSAVYYYMKIIERFPNTKYGKIASEKINKNM"
     gene            5503..5991
                     /gene="lptE"
                     /locus_tag="PLF90_03030"
     CDS             5503..5991
                     /gene="lptE"
                     /locus_tag="PLF90_03030"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOK56291.1"
                     /translation="MKRLIKICSLFLFFTSCYINKNISEKNRIYIDKIENYTNQSMLS
                     FTIREKIEKIILNYSGYTLTNMKEIADYTLSVKILKFERVPVFFDKKDIDNIVGAKYR
                     IEVQMKVNDGRKENAVLTKNLIEDISSSIYKEYREEKIFEKLSEQIAQRIYFELLKLK
                     NR"
     gene            5988..6620
                     /locus_tag="PLF90_03035"
     CDS             5988..6620
                     /locus_tag="PLF90_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56292.1"
                     /translation="MKNLIVIINDIEKVKTTIETLKEKYNINDFDIKIYDAEKIEINR
                     IINDFDYNPIFSENSLYIIKNVESFSKDDCEILYNSIKKLPPNIIVILYGISIKPPFK
                     ESKFKKEPVSPEEKFFKEIYNLKGTNRKKIMEILRNYMKVREKNFTPVISGIEIYLRN
                     ILYNEKTLKDEFIKKFDLLFNLDYSLKTGMIEVGSELHIYLFYYFFSGSS"
     gene            complement(6596..6883)
                     /gene="rpsT"
                     /locus_tag="PLF90_03040"
     CDS             complement(6596..6883)
                     /gene="rpsT"
                     /locus_tag="PLF90_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOK56293.1"
                     /translation="MAKKKRSVLKRQRQEIKRRLRNKMINSKIKTLIKKTKEAVLNNN
                     PEFENILKETIKEIDKAIAKGIIHKKTGARKKSRLMKFVKKYRVQLEPEKK"
     gene            7276..7485
                     /locus_tag="PLF90_03045"
     CDS             7276..7485
                     /locus_tag="PLF90_03045"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOK56294.1"
                     /translation="MIEEIKKEIDNLTERQKTVFILKTYEELTYEEIANILKSRVGTI
                     KATYFQSLQKIRENLKKKGVIKNEM"
     gene            7475..7930
                     /locus_tag="PLF90_03050"
     CDS             7475..7930
                     /locus_tag="PLF90_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56295.1"
                     /translation="MKCKNVEKNILDYIEGKVKEKEKIREHLEKCLKCRNLYKEFLLI
                     LNSLPKEEFSGFDENFWKEKFKYIQAKQSLHIKLKPVFAGISLFLIFFSTFLFTRIYT
                     SNLKKVNSTVKIEILNHNLPFSEEEMIEYVDYMQDEEAEKILDVIFKGF"
     gene            8050..8640
                     /locus_tag="PLF90_03055"
     CDS             8050..8640
                     /locus_tag="PLF90_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOK56296.1"
                     /translation="MEKIKLPSPDFEGKISVEESLKSRRSVRSYKDTPLSLKELSQIL
                     WATDGKTADWGGRTAPSAGATYPLEIYVVVGNVEGLSPGLYHYEIDSHSLTLVKEGDL
                     RTELSRAALNQTSIKLAPITIVISGIFERTTNRYGKRGERYVYMEVGHCGQNIHLQAE
                     SLGLGTVMIGAFEDEKVKKVLGIKEDVFYLCPVGKK"
     gene            8637..10340
                     /gene="polX"
                     /locus_tag="PLF90_03060"
     CDS             8637..10340
                     /gene="polX"
                     /locus_tag="PLF90_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOK56297.1"
                     /translation="MSKNQEIANIFDKIADALEFKGDDSFRIGAYRKAARILREYPED
                     VEEVYKKGEIQKIPGIGKGMAEKIEEYLKTGKMKKYEEAVKDIPEELLQLLEIPNLGP
                     KTLNLAYNQLGVKNLQDLEKVIENGQLAKLFGMGEKKVENIKKGIELFKQGRERIPIG
                     IALPIVEDIINELKKYTDNIDPAGSLRRMRETIGDIDILATSKNNIEIIERFVNLPIV
                     KEVIVKGNTKASIIEKSHNLQVDLRVVPNDSFGAALQYFTGSKAHNIKLRGFAKEKGL
                     KISEYGVFKGDEKIAGRTEDEVYKTLGLIWIPPELREDRGEIEAAIERKLPILVEEKD
                     IKGDLHIHSKYSDGVETIEEIAIAAKNLGYEYIGICDHSKTSKIAGGLDEDSLKRRNE
                     EIDKTNEKIKGIRVLKGMEVDILADGSLDYSDKILEELDFVIASIHQGFKKNVTERIK
                     KAIENPFVDIIAHPTGRLLSGREGYEVNIDEIIEYASKFNVALEINCYPDRLDLNDVN
                     ILKGKTKNLFFSLGTDAHNTGMLKYMKFGIGMARRGWLTKREILNTYKWENMPLRRRK
                     R"
     gene            10337..11197
                     /locus_tag="PLF90_03065"
     CDS             10337..11197
                     /locus_tag="PLF90_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOK56298.1"
                     /translation="MIPVIKIEGNSIAEVWEKSLLELWKNGISIKTEYDRKNDPPSKD
                     CTMIMVINEPFSEPRIHLAFPGGIEDLEKYRQEVVYGIHDAWINPEEKKWTYTYHDRL
                     TNYHIPGKEEKVNQLEYIINKLSETHFSRRAQGITWIPFYDPFTYDPPCLQRIWCRII
                     EKEGELFLNMNTHWRSRDAYRAAFMNLFGLTELQKYIADEISKKISKNVKVGQYVDIS
                     DSYHIYGESFEDFEKRFLTSLKKRDFYNPDHTKSRTLKTNDPVAVAGFEYGKQLIEME
                     KKTGKLGTLF"
     gene            11216..12139
                     /gene="miaA"
                     /locus_tag="PLF90_03070"
     CDS             11216..12139
                     /gene="miaA"
                     /locus_tag="PLF90_03070"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOK56299.1"
                     /translation="MKNKIVIIGGPTASGKTEISFKIAQKINGEIISCDSRQFYKEIN
                     IGTDKPSELMKKEIPHYFIDFLSLKDEFDVYKYSKQVFKKTEEILSRNKIPLIVGGSG
                     FYIRILLNGMFYIPDELKDKQKEIREKLEKEKTETLYEKLKIIDPEISKKIHPNDRYR
                     IKRALEVYELTGKNMTYWQNQKPEITLKNLGKIYYFILMRDRKIIYERIEKRVEKMFE
                     NGWIEEVKKLKEEGFEKYLNLKAPIGYNEIIEFLNGKYNFEELKRLIIKRTKEYARKQ
                     IIWFKKEKGIFINFLENEEAIEKIMGIYKND"
     gene            12132..12875
                     /locus_tag="PLF90_03075"
     CDS             12132..12875
                     /locus_tag="PLF90_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56300.1"
                     /translation="MIDWGSGYFAGLKLGFILIVLGIEFYFIIFEKWLEKDITHQEIV
                     IFSILFAISIVLTILKMSLISIFIPLSGYVFIQVLHEIKEKTLTEKMESRRIDELKEI
                     ILQQPNNYKAYVELGDIYFKKEEYSKALELYKTGYKIKDLPSIKQKILISEKENKIKR
                     GIIWICRNCGEENTEKEQKCKNCGEEKDVVKSIISDLKETKKYFFILIISPFIVLIIL
                     LVIRFLPLYLSIILFLFLLYLVFKFFLIS"
     gene            12933..14225
                     /gene="purB"
                     /locus_tag="PLF90_03080"
     CDS             12933..14225
                     /gene="purB"
                     /locus_tag="PLF90_03080"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOK56301.1"
                     /translation="MIPRYTRKEMADVWSEENKFKKYLEVEIAVLEGWSKIGVIPYDV
                     VEKIKNKAKIDIGRINEIEKITNHDVIAFVEQVSEEIGDEGKYIHLGLTSSDIIDTSF
                     ALILREAGNIILDDIKKLLEVLKDKAIQYKNTLMIGRTHGIHAEPITLGFKIGTWYFE
                     FLRNKERIEKAIDEISVGKISGAVGTFSNIELEIEEYVCDKFNLKREKFSTQIISRDR
                     YAYFITILGIIASSCEKVALQIRLLQQTEISEVFEPFGKGQKGSSAMPHKRNPVLCER
                     VCGLARVIKKNVNVALENVSLWHERDISHSSAERIILPESTILLDYILTLLTKIIDGM
                     VVNEENMKKNLNVLNNVYFSQKLLNCLVLKGLSRKEAYEKIQKISFEAINKKLDFCEL
                     VKKDDFIKKYIKDDELNKIFDINELLKNIEKIFKYIEK"
     gene            14228..15250
                     /locus_tag="PLF90_03085"
     CDS             14228..15250
                     /locus_tag="PLF90_03085"
                     /inference="COORDINATES: protein motif:HMM:NF021646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56302.1"
                     /translation="MKFILFTLHGYLELFKKRWEKTLFDYAKKENFELFMEKGEWGYV
                     LLDKTIEKSYFKFLLPEISFPGESFIRSLKYSIPSSENDTFFLCQFISVIENKIIETD
                     VNLNLKEKQKLIEEIKKESKINEFFVYEKDIILKVPENLPMLDSKFPNKIKNKYVDEI
                     KYKGENFEIINKLMDNSLKILNLHPINKIRVDLNEPIANFLHLYGMGKFNEKVYFSER
                     IKKNTFYFSDTKVLDGLRIFLGIEEITKIYPIKDDFFYWFDFALNPTDTPSIWVKKFE
                     AFSLEVLKKLSNLEDAKFLFIFDPFMNENYEYEKTFCLFLAVNFSRKLKRKYKDSSIL
                     FKSFIE"
     gene            15247..15711
                     /gene="folK"
                     /locus_tag="PLF90_03090"
     CDS             15247..15711
                     /gene="folK"
                     /locus_tag="PLF90_03090"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOK56303.1"
                     /translation="MKKERVYIAIGSNKGEKIKNIISGLKEIEKFLDIKKISFFYRNP
                     PVNAKGGIFLNGAIEVETNISPENLLEKLKNVEKKIGRKFPHQKGDAREIDFDIIFYG
                     KKIIKTKKLIIPHPEFKKREFVLKPLLEINCKLKDPVSNNKLKEIYRKLKKT"
     gene            15825..>17497
                     /locus_tag="PLF90_03095"
     CDS             15825..>17497
                     /locus_tag="PLF90_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007547212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOK56304.1"
                     /translation="MEENKQNLSSYDASAIQVLGGMEAVRKRPAMYIGDTSTGGLHHL
                     VFEVVDNSIDEAMAGYCDEINVIVRPENIVTVEDNGRGIPVDIHKTEKKPAVEVVMTT
                     LHAGGKFDKKVYKVSGGLHGVGVSVVNALSEYMEVEVKRDGKVYYQKYEKGKPVTSLK
                     IIGKTEKTGTKVTFKPDEEIFETIIFDENILIARLKELAFLNAGVKITFIDERKNIKE
                     EFKYEGGLISFVEYLNKNKKVLHPKPFYIKKTEEDIEIEAVFQYNDGYSETVISFANN
                     VNTREGGTHLTGFRSGLTRALNEYGKNNGFLDDLNLTGEDVKEGLTGIIAVKLPEPQF
                     EGQTKAKLGNSKVRFIVESIVYENVLTFLEENPEAGREILNKCIATAKAREAARKARE
                     LTRKKLLETGALPGKLADCSIKEPEKRELFIVEGDSAGGSAKQGRERTFQAILPLKGK
                     IINTEKASLEKVLNNDEIKTIISAIGGGVGDDFDISKVRYHKIIIMTDADVDGAHIKT
                     LLLTFFYRQMTDLIKHGYVYIAQPPLYKIKKGKKEFYIDTENEMDKVLV"
     assembly_gap    17498..17507
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17508..18198
                     /locus_tag="PLF90_03100"
     CDS             <17508..18198
                     /locus_tag="PLF90_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOK56305.1"
                     /translation="IKLFLSYDSKLSEYTEQIQTIFEISKKLIEIIKTLSLKGLDIKK
                     IVSFYKKNRKMPLYFLTYEDEELFLTDEEEVTKYLNKKGEEFDLFSKSENKKYKLFEI
                     YETKELQKIFKKLEEIDIKLDKIFEKIFVIKDDKGKEERVSIIEIYDKIKEKEKTGFI
                     IQRYKGLGEMNPEQLWETTMNPQTRRLKKITIEDAIKAEEIFTILMGEVVEPRREFIE
                     KYAREVKNLDI"
     gene            18211..>19404
                     /locus_tag="PLF90_03105"
     CDS             18211..>19404
                     /locus_tag="PLF90_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOK56306.1"
                     /translation="MEGEKIVTIGIEEEMKESYLDYAMSVIIGRALPDVRDGLKPVHR
                     RILYGMLELGLEPGKPFKKSARIVGEVMGKYHPHGDAPIYEAIARMAQPFSLRYPLIE
                     GQGNFGSIDGDPPAAMRYTEARLASITMEMLADIEKETVDFVPNFDESLIEPTVLPSK
                     IPNLLINGSSGIAVGMATNIPPHNLSEVVNAMIKLIDNPEITIEELIEILPGPDFPTG
                     GMICGREGIKSAYTTGKGIIIVRGHVDVEEEKNKVNIVIKTIPYEVNKSNLVEEIANL
                     IENGKIGGVTEVRDESDKNGIRVVLETKKGENVDIIINQLYKHTQLQTSYGIINLALV
                     NRQPRLLNLKEMLQLFINFREEIVIKRTRYDLKKAEERYHILLGLIIALDNLDNVINL
                     IRSSKN"
BASE COUNT         8185 a         2013 c         3083 g         6113 t
ORIGIN      
        1 catcaattgg attgaattat gctcctccaa atagaaatct tctttcatta agagaagttc
       61 tgcttataaa aggatttgat aaagaaaagg tttatgggaa taaggagaaa aaaggacttt
      121 ttgactttat aacaatatat tctgatgata aaataaatat aaatacttgt aaaagcgaat
      181 tattaaattc tatgggtttt acaaatgaag atataagtaa aataatggaa gagagagaaa
      241 atagaccgat taatgaaaca attcttttga atataaacag gaatgttttt ttgaaaaata
      301 aaaatttaat aaaatttaaa agtaattatt ttcatatctc attaaaagtt aaggatgaaa
      361 aaggaaaaga aagaaatgtg gaaggaattt taaaaaaaga taaaactatt gagttaataa
      421 aaaagggtat tttatgaaaa tttttttata tttaaaaggc gaaaaagttt attattttaa
      481 gaacaatgaa agaggtgaaa ttccttttga aaatctggaa aactttataa aagaaaataa
      541 acagaacttt ttttatttaa ttttttcaaa attaaatatt tattttagaa aagtagaatt
      601 tgaattcagg gataagaaaa aaataagttt agttctaaat caggaaattg aaggtaaatt
      661 tcctacatca atagacaagt tatatttcca ttttaaattt ttttatcctg aaaaagataa
      721 aactaccatt gggattttcg ctgttgaaaa agaaaaaata gaatatttaa aggaaatttt
      781 caaaaaaaat aaaacaaaat ataaattttt aattgattca attttaattc atcaattttt
      841 tagaaacaac ttaaaagaat acgattttat tgaactttac actgaaaaag attatttatt
      901 aataaatctt attgaaaatt caactatttc cggtgtttat tcctactttt caaataacat
      961 aaaagaaagt attttagaag ttttgccttc tttatttgta acaaaaaaac ttcctgttta
     1021 ttttataggc gatagaaaaa tctatgaaga aataaaaatg gacggattac actttttctc
     1081 tgaaacaaat ttttttaata tcttaaatga attaaaaaaa cttgaagttg ttaatttcaa
     1141 agatattctt ataagaaaga aaatattgaa ttttgaatat ctgtcttact ttattatttt
     1201 acttgtaagt acttttcttt tttttacacc tcattttaaa attactgaaa aagaaaagaa
     1261 attagatgaa attaacaaaa aaatggaaaa tatttttaaa tcactatttc ctgaaacaga
     1321 aaaaattgtt aatcctttga tacagattaa agaaaaattt atacaggata aaagttattc
     1381 aaaaatacct gtatatgaga tttctgttat taaaacactt gaagaaataa cacttttttt
     1441 tccagaaaat attaatggag aggttgaaga agtatcaata aatgataaaa atattaacct
     1501 aacaggaata gttgataatt taaagaatct tgataatctt aaagaaaata ttagaaattc
     1561 aaaatttttt aagaattttg aaattaacag tgtatcattt accaaagaaa ataaaattag
     1621 atttaatctt ttattgagga cagggactaa tggatagaaa aatcatttta acttttgtta
     1681 tttttattat tatttttgaa tatcagtata tttatttacc aaataagaaa aaaattaaaa
     1741 atcttgacag tattatttta aaaaaggaaa aagaatataa agagtttatt gagttatgtg
     1801 aaaaatataa aaagggagag gtggaaaaaa aagatactac tgtgaatgtt gtttcagata
     1861 aattttcttt cttttcttat ttaaatgatt taattgaaaa ttcctcaata aaaacgaatt
     1921 ttggagatat taaaatttta cccagagaag aaatagatga atatattatg gaaaaaatac
     1981 aaattgatat aaatctcatt acccttgaac aacttctttc aatacttaat aaaattgaaa
     2041 aaacaaaagg aatttatata tcccaatttg aaatgaagag agataaaaat aaaccatatc
     2101 ttttaaatgt ttcaatgata ataacttgtt taaaaaatag aattacagac aatttttaat
     2161 aatggaagac atatatctta tagatggaac ctgcttaatc tatagaagtt tctatgcttt
     2221 accgaaatta aaaacttcta cgggaataat aacaaatgca atatatggtt ttaccacaac
     2281 attacttaaa attttaaaag aaaaaaaacc tgaatatatg gctatttttt ttgaccttca
     2341 agtaccaact tttagacata taacttttga aaaatataaa gaaaaaagga aaccaatgcc
     2401 tgatgaactt tctggacaga ttcccaaaat aaaagaaata attacctatc ttggtataaa
     2461 atattatgaa aaagaaggat acgaagcaga tgaccttatt gccagttcaa ttgaaaaatt
     2521 aaagtctgat gacagggtat tctttataat atcaagtgat aaagatattt tgcaactaat
     2581 agataaaaat gttttcgtta taaatccaat agattacagt attttagacg aaaaatcagt
     2641 aattaataaa tatggattta gaccggaaaa aatagttgat tttatagggc ttgcaggaga
     2701 tacttctgat aatattccag gaatacctgg aattggagaa aaaactgcaa tttctcttct
     2761 ttcaaaattt aattcaattg aagaaattta taataatctt gaaaaaattg attcaaaaaa
     2821 tttaagagat aaactcgaaa aaaatagaga aattgccttt ttaagtaaaa aactttcaac
     2881 tttaaacaaa aatttttttg ataaaattac tcttgaagag ataaaaataa attctcctga
     2941 tttaccaaga ttatatgaaa tttttgaata ttttgaattt actaaactta aagaaattat
     3001 aaaagaaatt tatcccgatg ttgagaatat tgaaagcata gaaaatttta taggattttc
     3061 tacaggagaa atcataaact atactgaaat actaagatat ccagaaaaat ataaaaagat
     3121 tcttgaatgc gatgatataa agaaaattgg ttttgacctc aagagtaaaa ttgtagactt
     3181 gataaataag ggtattaaac tcaaaaatat tgattttgat ttttctattg ccaaacatct
     3241 tacaggaaaa gcaatttata atagaaattt atttgagttg aaaaaagaat atgaaaattt
     3301 attgataggt tttaatatgt ataaactatt tgaagaagtc gaaatgcccc ttatctatgt
     3361 tcttgtatgg atggaaatta caggtataaa agttgatatt gagtatctaa aaaatttgaa
     3421 tgaaaaattt aatatagaaa taaatgaaat tatagataag atttattctt taagtggtga
     3481 agtatttaat ctaaattctc ctaatcaagt aggcgaaatt ctttttaaaa aattaaaact
     3541 tcctgttata aaaaaaacga aaactgcata tgcaacagat atcaatgtat taaaacaact
     3601 ttcccctctt catccattac caaaacttct tcttgaatac agggaacttt ataaaataaa
     3661 atccacatat attgaaggtc ttttaaattt tattaatcaa aaaacaggaa ggatacatcc
     3721 aacatacagt caaatttcta caacaactgg tagattgacc tgttcaaatc caaatttgca
     3781 aaatctacct gttaagtccc aaaggggtgg tttaatcagg aaggcatttt gtgcagaaaa
     3841 aaataatttt cttttatctt ttgattacag tcaaatagaa ttaagagtcc ttgctcattt
     3901 ttcagaagac cctgttttaa ttgatgcttt tgaaaaagat agagacatac actcagaaac
     3961 tgctgaaatt attctcacca gtgactctct tttttctcca ttaaattttg gagaacttac
     4021 aaaagaagaa aaaagaagaa ttgcaaaaac aataaatttt ggaattatat acggaataag
     4081 cccttatggc ctttcacagg aactcggaat atcagttgaa gaaagttcaa tttttattca
     4141 aaaatatttt gaaaaattca aaaaagttaa acaatatact gaaaacataa taaaaaaagc
     4201 agaagataat ggctatgttg aaacactgct aaaaagaaga aggtatattc cagaattgaa
     4261 aagtcaaaat aaaaatgaaa gagaatttgg caaaagagtt gcaataaata tgccaattca
     4321 ggggtctgct tctgaaatta taaaactatc aatgaataaa atatatcaaa aatttaaaga
     4381 agaaaattta aaaagtaatc taattctcca aattcatgac gaacttttat ttgaagtttt
     4441 accagaagaa gaaaaaaagg ttatagaaat tgtaaaggag attatgaaaa atgctataat
     4501 cttaaaagta ccaattaaag ttgatgtaaa aaaaggtaaa aattttcttg aaatggagga
     4561 gataaatgat tgacataagg atgtatttat gtaaaaattt ttacatgagg aaatttcaaa
     4621 tttttttaat gtttttttta attgtatcct tatcttattc ctactgggaa tggacaccac
     4681 agacaaaaaa atggataaat cctaaatatg ctgtaaaaga aactcctgaa gaacaattta
     4741 aatatgcaga ggaattaaga aaaaacggaa aaattgaagc atctataaga gaacataaga
     4801 aattattaaa acattattca aaatcagaat acgctccttc atcatgtttc attttaggcg
     4861 aaatatacag ggaaaaggga gatataaaaa aagcatttga ttattatcaa aaaattatag
     4921 atgaatatcc ttcttcacct cttgttcttt cttcaattaa aattcaatca gaaatagcag
     4981 aaaaaaaatt agagagtaaa aaaggattat tcagaggttt attttcaaga gcagaagaaa
     5041 aggcacaatt tatgaataaa gttatagaaa atagtccata cgatttggaa gcagtggata
     5101 gaatgtttaa acttgcagat ttttattttg atttaaaaga atatgataaa agtatagaag
     5161 ttttagaaaa aattataaaa aattttcctg aaacatctaa tgcagaaaga gccaaatttt
     5221 taaaaataaa atatttacta aatttaatac ccgaagtttc tcttgatata gatacaatag
     5281 aagatataaa agaccagata aatgaatttc taattgaata tccaaacagt agatttgaaa
     5341 atgaaattaa aaaaatagaa attatgcttg atgaaaaaga ggcagaaaaa tattatcaaa
     5401 ttgcaagata ttatgaaaga gcaggaaaga aaaaaagtgc agtttattat tatatgaaaa
     5461 taatagaacg gtttccaaat actaagtatg ggaaaattgc aagtgaaaag attaataaaa
     5521 atatgtagtt tgtttttatt ctttacttcc tgttatataa acaaaaacat ttcagaaaaa
     5581 aacagaatat atattgacaa aatagagaat tatactaatc aatctatgtt aagttttact
     5641 attagggaaa aaatagaaaa aattatttta aattattcag gttatacatt gacaaacatg
     5701 aaagaaattg ctgattatac actttctgtt aaaattctta agtttgaaag agttcctgtt
     5761 ttttttgata aaaaggatat tgataatata gttggtgcaa aatatagaat tgaagtacag
     5821 atgaaggtga atgatggaag aaaagaaaat gcagtcttaa ctaaaaattt gattgaggat
     5881 atttcctctt ctatttataa agaatacagg gaagaaaaga tatttgaaaa actttctgaa
     5941 cagattgctc aaagaatata ttttgaactt ttaaaactta aaaacagatg aaaaacttga
     6001 tagttattat aaatgacatt gaaaaagtaa aaacaaccat tgaaactttg aaagaaaaat
     6061 acaatataaa cgattttgat ataaaaattt atgatgctga aaaaattgag ataaatagaa
     6121 taataaatga ttttgattat aatccaatct tttctgaaaa ctccctctat ataataaaaa
     6181 atgtagaaag tttttctaaa gatgattgtg aaatacttta taattcaatt aaaaaactcc
     6241 ctccaaatat aattgttata ctttatggaa tttcaataaa accaccattt aaagaaagca
     6301 aatttaaaaa agaacctgtt tctccagaag aaaaattttt taaagaaata tataatttaa
     6361 aaggaacaaa tagaaaaaaa ataatggaga tcttaagaaa ttatatgaaa gtcagggaga
     6421 aaaactttac tcctgttata agtggtattg aaatctattt aagaaatatt ttatataatg
     6481 aaaaaacatt aaaagatgaa tttataaaaa aatttgattt acttttcaat cttgattact
     6541 ctttgaaaac aggaatgatt gaagtgggtt cagaattaca tatatatctt ttttattatt
     6601 ttttttctgg ttcaagttga accctgtatt tctttacaaa cttcatcaat ctgctttttt
     6661 ttcttgcacc ggttttttta tgaattatcc cttttgctat agctttgtct atttccttga
     6721 tagtttcttt taaaatattc tcaaattccg ggttattatt caaaactgct tcttttgttt
     6781 tcttaatcaa tgtttttatt ttagaattta tcattttatt tctcaatctt ctttttattt
     6841 cctgtctctg tctttttaaa acactccttt ttttcttagc catatataac ctcctctaat
     6901 ttgcggtata atatatcata aaataaacca tcaatcaagt tttaatgtct aaattaaaat
     6961 gaaggataaa aaaagttttg aggaatatta tataatgtat gcaaataaaa tttatggaat
     7021 agcttttaga atggtaaata atcatagtga tgcattggat atagttcaag aatctttttt
     7081 acgcgcttat aaaaactgga acaactttaa aggactttct aaattttcaa cttatcttta
     7141 tagaatagca gtgaatttat catatgattt ttaagaaaaa aaaatagaat aaaaaaaatt
     7201 gaaaattata attatgaaaa tatttttcat aacagtaaca gaaaaaattt tgacgattgt
     7261 attcgttgtg gtgatatgat agaagagata aaaaaagaga ttgataattt aacggaaaga
     7321 caaaaaacag tttttattct gaaaacatat gaagaattga cttacgaaga aatagcaaat
     7381 attttaaaaa gtagagttgg aacaattaaa gcaacttatt ttcaatcgtt acaaaaaata
     7441 cgagaaaatt taaaaaagaa aggggtgata aaaaatgaaa tgtaaaaatg tagaaaaaaa
     7501 tattttggat tatattgaag ggaaagtaaa agaaaaagaa aaaattagag aacatctgga
     7561 aaaatgttta aaatgtagaa atttatataa ggaatttctt ttaattttaa acagtttacc
     7621 caaggaggaa ttttctggtt ttgatgaaaa tttctggaag gaaaaattta aatatattca
     7681 agcaaaacaa tctttacata ttaaattgaa acctgtattt gcaggtattt cactcttttt
     7741 aatatttttt tcaactttct tatttaccag aatatatact tccaatttaa aaaaagttaa
     7801 ttctactgta aaaattgaaa ttttgaatca taatttgcct ttttcagaag aagaaatgat
     7861 tgaatatgtt gattacatgc aagatgaaga agcagaaaaa attcttgatg taattttcaa
     7921 gggcttttaa aagtactatt ttctctataa aaaacttgaa aaatttctca attaaaaata
     7981 aaattaatta caatgaaaac atatatttta ttatttttta tcatttctgt tataattggg
     8041 ggtgaaaata tggaaaaaat taaattacca tctcctgatt ttgaaggaaa aattagtgta
     8101 gaggaatctc ttaaaagtag acggtctgta agaagttata aagatacacc attaagttta
     8161 aaagaattgt cccagatttt atgggcaact gatggaaaaa ctgctgactg gggtggaaga
     8221 actgcacctt ctgcaggtgc tacatatcct cttgaaattt atgtagtagt tggaaatgtt
     8281 gaaggattat caccaggact ttatcattat gaaattgatt ctcactcatt aaccttagta
     8341 aaagaaggag atttaagaac agaactatca agagcagcat taaatcaaac atctataaaa
     8401 cttgcaccta ttacaatagt tatttctggg atttttgaaa ggacaacaaa tagatatgga
     8461 aaaagaggag aaagatatgt ttatatggag gtcggtcatt gcggtcagaa tatacactta
     8521 caggcagaat ctctcgggct tggaactgtt atgataggtg catttgaaga cgaaaaagtg
     8581 aaaaaagttc ttggaataaa agaagatgtt ttttatttat gtcctgttgg aaaaaaatga
     8641 gtaaaaatca ggaaattgca aatatttttg ataagattgc agatgctctt gaatttaaag
     8701 gagatgattc ttttcgtata ggtgcttata ggaaagcagc aaggatttta agagaatatc
     8761 cggaagatgt agaagaagtt tataaaaaag gagagattca gaaaattcct ggtataggta
     8821 aaggtatggc tgaaaaaatt gaagaatact tgaaaacagg taaaatgaaa aaatatgaag
     8881 aagcagtaaa ggatatacca gaagaacttt tgcaacttct tgaaatacca aatcttggtc
     8941 caaaaacatt aaatcttgca tataaccaac taggtgtaaa aaatttacag gacctagaaa
     9001 aagtaattga aaatggacaa cttgcgaaac tttttggtat gggagaaaaa aaagttgaga
     9061 atataaaaaa aggaattgaa ctatttaaac agggaagaga aagaatacct attggaattg
     9121 cactgcctat tgtggaagat ataataaatg aattaaaaaa atatacagat aatattgacc
     9181 ctgcaggttc attaagaaga atgagagaaa caattggaga tattgatata cttgcaacta
     9241 gtaagaataa tattgaaatt attgaaaggt ttgttaattt accaatagta aaagaagtta
     9301 tagttaaagg aaataccaaa gcatcaataa tagaaaaatc acataattta caggttgatt
     9361 taagagtagt tccaaatgat tcctttggag cagctcttca atattttacc ggttcaaagg
     9421 cacataacat aaaattaagg ggatttgcaa aagaaaaagg tttaaaaatt agtgaatatg
     9481 gagttttcaa aggtgatgaa aaaattgcgg gcagaactga ggatgaagtg tataaaactc
     9541 ttggtcttat ctggattcca cctgaactaa gagaagacag aggagaaatt gaagcagcaa
     9601 ttgaaaggaa attacctatc cttgttgaag aaaaggatat aaaaggagac cttcatattc
     9661 attcaaaata ttcagatgga gttgaaacaa tagaagaaat tgcaattgct gcaaaaaatc
     9721 tggggtatga atatattgga atatgtgacc attcaaaaac atcaaaaatt gcaggtggac
     9781 ttgatgaaga tagtttgaaa agaagaaatg aagagataga taaaacaaat gaaaaaataa
     9841 aaggcataag agttttaaaa gggatggagg ttgatatact tgctgatggt tctcttgatt
     9901 attctgataa aattcttgaa gaacttgatt ttgttattgc ttcaattcat cagggattta
     9961 aaaagaatgt aacagaaaga ataaaaaaag caattgaaaa tccatttgtt gatataattg
    10021 ctcatccaac aggtcgtctt ttaagtggga gagaagggta tgaagtcaat atagatgaaa
    10081 ttattgaata tgcttcaaaa tttaatgttg cccttgaaat aaattgctat ccagatagat
    10141 tggatttaaa tgatgtgaat attcttaaag ggaagacgaa aaatttattt ttttctcttg
    10201 gtacagatgc tcataataca ggaatgttaa aatatatgaa atttggaatt ggtatggcaa
    10261 gaagaggatg gcttacaaaa agagaaattt taaatacata taaatgggaa aatatgcctt
    10321 taaggaggag aaaaagatga tacctgtaat taaaattgaa gggaattcaa tagcagaagt
    10381 atgggaaaaa agtttacttg aactctggaa gaatgggatt agtataaaaa cagaatatga
    10441 taggaaaaat gaccctccaa gcaaagattg tacaatgatt atggttataa atgaaccttt
    10501 ttctgaacca agaattcatc ttgcttttcc aggaggaatt gaggaccttg aaaaatacag
    10561 acaggaagtg gtttatggaa tccatgatgc atggataaat cccgaagaaa aaaaatggac
    10621 ttatacctat catgaccggc ttacaaatta tcatatcccg ggaaaagaag aaaaggttaa
    10681 tcaacttgaa tatataataa ataaactatc agaaacacat ttttcaagaa gggcacaggg
    10741 gattacatgg attccttttt atgacccatt cacctatgac cctccatgtt tgcagagaat
    10801 atggtgtaga attattgaaa aagagggaga attattttta aatatgaata ctcactggcg
    10861 ttcaagagac gcttacagag ctgcttttat gaatttattt ggtctgaccg aacttcagaa
    10921 atacatcgca gatgaaattt ctaaaaaaat aagtaaaaat gttaaagtag gacagtatgt
    10981 tgatatttcg gattcctatc atatttatgg agaaagtttt gaagattttg aaaaaagatt
    11041 tttaacttct cttaaaaaaa gggattttta taatccagac catacaaaaa gtagaacatt
    11101 aaaaactaac gacccggtcg cagttgcggg ttttgaatat ggaaaacaac ttatagaaat
    11161 ggaaaagaaa acaggaaagt taggaactct attttaaatc acaggcatat atttaatgaa
    11221 gaataaaata gtaatcattg gaggtccaac tgcctctgga aaaacagaaa tatcttttaa
    11281 aattgcacaa aaaataaatg gagaaattat atcctgtgat agtagacaat tttataaaga
    11341 aataaatata ggtacagata aaccttctga attaatgaag aaagaaattc cacattattt
    11401 cattgatttt ctatcattaa aagatgaatt tgatgtatat aaatattcaa aacaggtttt
    11461 taaaaaaaca gaagaaatac tttcaagaaa taaaattcct ttaattgttg gtggaagtgg
    11521 attttatata agaattttat tgaatggaat gttttatatt cctgatgaat taaaagataa
    11581 acagaaagaa atcagagaaa aacttgaaaa agaaaaaact gaaactttat atgaaaaatt
    11641 aaaaattatt gaccctgaaa tatcaaaaaa aatacatccc aatgataggt atagaattaa
    11701 aagagcactt gaggtttatg aattgactgg aaaaaatatg acttactggc aaaatcaaaa
    11761 acctgaaatt acattgaaaa atctaggaaa gatttactat tttattttaa tgagagacag
    11821 aaaaataatt tatgaaagaa tagaaaaaag agtggaaaaa atgtttgaaa atggatggat
    11881 tgaggaagta aaaaaattga aagaagaagg ttttgaaaaa tatttgaatt taaaagctcc
    11941 tattggatat aatgaaataa ttgaatttct aaatggtaaa tataattttg aagaattgaa
    12001 aagattaatc attaaaagga caaaagaata tgcaagaaaa cagattatat ggtttaaaaa
    12061 agaaaaagga atatttataa attttttaga aaatgaagaa gcaattgaaa aaataatggg
    12121 aatatacaaa aatgattgat tggggttctg gatattttgc agggttaaaa ctgggattca
    12181 tccttatagt tctgggaata gaattttatt ttattatttt tgaaaaatgg ttggaaaaag
    12241 atataaccca tcaagaaatc gtaatctttt ctattctttt tgctataagt attgtattga
    12301 ctatattaaa aatgtcattg atttcaatat ttattcctct ttctggatat gttttcattc
    12361 aagttttaca tgaaataaaa gaaaaaactt taacggaaaa aatggaatcc agaagaattg
    12421 atgaactgaa agaaataata cttcaacagc caaacaacta taaggcatat gtagaactgg
    12481 gagacattta tttcaaaaaa gaagagtact ctaaagcact tgaattatat aaaacagggt
    12541 ataaaattaa agatctacca tcaattaagc aaaaaatatt aatatcagaa aaagaaaata
    12601 aaataaaaag gggtattatt tggatttgta gaaattgcgg tgaagaaaac acagaaaaag
    12661 aacaaaagtg taaaaactgc ggtgaagaaa aagatgttgt taaatcaatt ataagtgatt
    12721 taaaagaaac aaaaaaatat ttttttattc ttataatttc tccttttata gttttaataa
    12781 tacttttggt aataaggttt ttacctttat atctatcaat aattttgttt ttgtttttat
    12841 tatatctcgt ttttaagttt tttcttatct cttgaaaaac ttattttgca acttaaatat
    12901 cgataatata taatttttac aaggaggttg atatgatacc aagatataca agaaaagaaa
    12961 tggctgatgt ctggagtgaa gaaaacaaat ttaaaaaata tcttgaagta gaaattgctg
    13021 ttcttgaagg atggagtaaa attggagtta taccatatga cgttgttgaa aaaattaaaa
    13081 ataaggcaaa aattgatatt ggaagaataa atgaaattga aaaaattaca aatcatgatg
    13141 ttattgcatt tgttgaacag gtgagtgaag aaattggtga tgaaggtaaa tatattcatt
    13201 taggacttac atcttcggat attattgata cttcttttgc attaatttta agagaagcag
    13261 gaaatataat tctggatgat ataaaaaaat tacttgaagt tttaaaggat aaggcaattc
    13321 aatacaaaaa tactttaatg ataggaagaa ctcatggaat acatgctgaa ccgataactc
    13381 ttggatttaa aattggaaca tggtattttg aatttttgag aaataaagaa agaattgaga
    13441 aagcaattga cgaaataagt gtgggaaaaa tatcaggtgc agtaggaact ttttcaaaca
    13501 tagaacttga aattgaagaa tatgtatgcg ataaatttaa tttaaaaagg gaaaagtttt
    13561 caactcaaat aatttcaagg gatagatatg cttattttat aacaattctt ggaattattg
    13621 cttcttcgtg tgaaaaagtt gctttgcaga taagattgct tcagcaaaca gaaatttctg
    13681 aagtttttga accatttgga aaaggacaaa agggctcttc tgctatgcca cataaaagaa
    13741 atcctgtttt atgtgaaaga gtttgtggac ttgcaagagt cataaagaaa aatgtaaatg
    13801 tagcattaga aaatgtttcc ttatggcatg aaagagatat aagtcattct tctgctgaaa
    13861 gaataatttt accagaatca acaatccttc ttgattatat tttaacatta cttacaaaaa
    13921 ttattgatgg aatggtagta aatgaagaaa atatgaaaaa aaatttgaat gttttaaata
    13981 atgtttattt ttctcagaaa ttattaaatt gtcttgtttt aaaaggtctt tcaagaaaag
    14041 aggcatatga aaaaattcag aaaatatcat ttgaggcaat aaataaaaaa cttgattttt
    14101 gtgaacttgt taaaaaagat gattttataa agaaatatat taaagatgac gaactaaata
    14161 aaatttttga tataaacgaa cttttgaaaa atatagaaaa aattttcaaa tatattgaaa
    14221 aataaagatg aaatttattt tatttactct tcacggatac cttgaactat tcaaaaaaag
    14281 gtgggaaaaa acattatttg attatgcaaa aaaagaaaac tttgaattat ttatggaaaa
    14341 aggagaatgg ggttatgttt tacttgataa aacaattgaa aaaagttatt ttaaatttct
    14401 attacctgaa atttcttttc caggagaaag ttttatcagg tctttaaaat actcaatacc
    14461 atccagcgaa aatgacactt tttttctgtg tcaatttata tctgtgattg aaaacaaaat
    14521 aattgaaaca gatgtaaatc taaatttaaa agagaaacaa aaacttattg aagaaattaa
    14581 aaaggaaagc aagataaatg aattttttgt ttatgaaaaa gatataattt taaaagtccc
    14641 tgaaaattta cctatgttag atagtaaatt tccaaataaa ataaaaaata aatacgtaga
    14701 tgaaattaaa tataaaggcg aaaattttga aataataaat aaacttatgg acaattcatt
    14761 aaaaatttta aatctacatc ctataaataa aatcagggtt gacttgaatg aacccattgc
    14821 taacttttta catttatacg gtatgggaaa atttaatgaa aaagtttatt tttctgaaag
    14881 aattaaaaaa aatacattct atttttcaga tacaaaagtt cttgatggtt tgagaatatt
    14941 tcttggtatt gaagaaataa caaaaattta tccaataaaa gatgacttct tttattggtt
    15001 tgattttgct cttaatccaa ctgatactcc ttcaatctgg gtaaaaaaat ttgaagcatt
    15061 ttctttagag gttttgaaaa aactgtcaaa tctggaagat gcaaaatttt tatttatctt
    15121 tgacccattt atgaatgaaa attatgaata tgaaaaaaca ttctgtctat ttcttgctgt
    15181 aaatttttca agaaaactaa agaggaaata taaagattcg tcaattttat ttaaatcttt
    15241 tattgaatga aaaaggaaag agtatatatt gccataggaa gtaataaagg ggaaaaaata
    15301 aaaaatataa tatcaggttt aaaagaaatt gaaaagtttc ttgatataaa aaaaatatct
    15361 tttttttaca gaaatccacc ggttaatgca aaaggtggaa tttttttaaa cggcgcaata
    15421 gaggttgaaa caaatatatc acctgaaaat cttttagaaa aattaaagaa tgttgaaaaa
    15481 aaaatcggga gaaaatttcc tcatcaaaaa ggtgatgcaa gagaaattga ttttgatata
    15541 attttttatg ggaaaaaaat aattaaaaca aaaaaactta taatacctca tccggaattt
    15601 aaaaaaagag aatttgtatt gaaaccactt cttgaaataa attgtaaatt aaaagaccct
    15661 gtatcaaata ataaattgaa agaaatttat agaaaactta aaaaaacttg aatattataa
    15721 aagtacataa aaatttatca aacctatcaa ctgtaaagca ttttattttg acagaaattt
    15781 taaaaaatgg taaaattttt tgttaaaaat ggaggttttt ttaaatggaa gagaataaac
    15841 agaatttaag ctcttatgac gcttccgcta tacaggtttt aggaggaatg gaagcggtta
    15901 gaaaaagacc tgcaatgtat attggagaca cttcaacagg tggactccat catcttgttt
    15961 ttgaagtagt tgataatagt atagatgaag caatggctgg gtactgtgat gaaataaatg
    16021 taattgttcg tcctgaaaat attgtaacag ttgaagataa tggaagaggt atacctgtag
    16081 atatacacaa aacagaaaaa aaacctgcag tagaagttgt aatgaccact ttacatgcag
    16141 gtggtaaatt tgacaaaaaa gtatataaag tttctggagg gctgcatggt gtaggcgtat
    16201 ctgttgttaa tgctctatca gaatatatgg aagtagaggt taaaagagac gggaaagtat
    16261 attatcaaaa atatgaaaaa ggaaaacctg ttacatcttt aaaaattata gggaaaacag
    16321 aaaaaacagg aacaaaggta acatttaaac cggatgaaga aatttttgaa acaattattt
    16381 ttgatgaaaa tatattgatt gcacggctta aagaacttgc ttttttaaat gcaggtgtaa
    16441 aaattacatt tatagatgaa aggaaaaata taaaagaaga atttaaatat gaaggtggat
    16501 tgatttcatt cgtagaatac cttaataaaa ataaaaaggt tcttcatcct aaaccatttt
    16561 atataaaaaa aacagaggaa gatattgaaa ttgaagcagt ttttcaatat aacgatggat
    16621 acagtgaaac cgttatttcc tttgcaaata atgttaacac aagagaaggg ggtacacatt
    16681 taacaggttt tagaagtgga ctaacaagag cattaaatga atatggaaaa aacaatggat
    16741 ttttagacga tttgaattta acaggagaag atgttaaaga gggattaaca ggaataatag
    16801 cggttaagtt acctgaacct caatttgaag gtcaaacaaa agcaaaactt gggaactcaa
    16861 aggtaagatt tattgtagaa agcatagttt atgaaaatgt tttgacattt cttgaagaga
    16921 atccagaagc aggtagagaa attttaaata aatgtattgc aaccgcaaaa gcaagagaag
    16981 cagcaagaaa agcaagagaa cttacaagaa aaaaactgtt agaaacgggt gcacttccgg
    17041 gtaaacttgc tgactgttca ataaaagaac ctgaaaaaag agagttattt atagttgaag
    17101 gtgattctgc tggtggaagt gctaaacagg gaagagaaag gacttttcag gcaattttac
    17161 ctttgaaagg taaaataatt aatacagaaa aagcatctct tgaaaaagta cttaataacg
    17221 atgaaattaa aacaataata agtgccatag gaggtggagt aggagatgat tttgatatta
    17281 gtaaagtaag ataccataaa atcataataa tgacggacgc tgatgtagat ggagcccata
    17341 taaaaacact ccttttaact tttttctata gacagatgac tgaccttata aaacatggat
    17401 atgtctatat agcccagccc cctctttata aaataaaaaa aggaaaaaaa gagttctata
    17461 tagacacaga aaatgaaatg gataaagtac ttgtacannn nnnnnnngat aaaacttttt
    17521 ttaagttatg actcaaaatt aagtgaatat accgaacaaa ttcaaactat ttttgaaata
    17581 agcaaaaaac ttatagaaat aataaagaca ttatccttaa aaggacttga tataaaaaaa
    17641 atagtttctt tctacaaaaa gaatagaaaa atgcccttat actttcttac atatgaagat
    17701 gaggaattat ttttaactga tgaagaagaa gtgaccaaat atttaaataa aaaaggtgag
    17761 gaatttgacc tcttttctaa aagtgaaaac aagaaatata aactttttga gatatacgaa
    17821 actaaagaat tacagaaaat ttttaaaaaa ttagaagaga tagacataaa acttgataaa
    17881 atttttgaaa agatatttgt gataaaggat gataaaggaa aagaagaaag agtttctatt
    17941 attgaaatat atgacaaaat aaaagaaaaa gaaaaaactg gttttataat tcagagatat
    18001 aaaggtcttg gagaaatgaa tcctgaacaa ctctgggaaa ccacaatgaa tccccagacg
    18061 agaagattaa aaaagattac tattgaagat gctataaaag cagaggaaat atttacaatt
    18121 cttatgggag aggttgttga accaagaaga gaatttattg aaaaatatgc gagagaagtt
    18181 aaaaatcttg acatttaaaa agaggtaatt atggaagggg aaaaaatagt aacaatagga
    18241 attgaggaag aaatgaagga gtcatatctt gattatgcta tgagtgttat tataggaaga
    18301 gctttacctg atgtaaggga tggattaaaa cctgttcata gaaggatcct ttatggaatg
    18361 cttgaattag gattagaacc tggaaaaccg tttaaaaaat cagcaagaat agttggtgag
    18421 gttatgggta agtatcatcc ccatggtgat gcccctattt atgaagcaat agcaagaatg
    18481 gcacaaccgt tttctctaag atatccctta attgagggac aggggaattt tggttctatt
    18541 gatggagacc cacctgctgc tatgagatat acagaggcaa gactcgcatc aattactatg
    18601 gaaatgcttg cagatattga aaaagaaaca gttgattttg tccctaactt tgatgaatct
    18661 ttgattgaac caactgtatt accaagtaaa attccgaatc ttcttataaa tgggtcttct
    18721 ggaatagcgg ttggaatggc tacaaatatt ccaccccata atttatcaga agtagtgaat
    18781 gccatgataa aattaattga taatcctgaa ataacaattg aagaactgat tgaaatttta
    18841 cctggtcctg attttccaac aggtggtatg atatgtggac gagaaggcat aaaaagtgca
    18901 tatactacag gaaaagggat aattattgta agagggcatg ttgatgttga agaagaaaaa
    18961 aacaaagtta acatagtaat aaaaacaatt ccttatgaag taaataaatc aaatcttgtt
    19021 gaagaaatag caaatctaat tgaaaatgga aaaataggag gagtaactga agtaagagat
    19081 gagtcagata aaaatggtat tagagttgtt cttgagacaa aaaaaggcga gaatgtggat
    19141 attataataa atcaacttta taaacacact caactacaga catcttatgg aattattaat
    19201 cttgcacttg ttaatagaca gcctcgtctt ttaaatctta aagagatgct tcaactcttt
    19261 ataaatttta gagaagaaat agttataaaa agaaccagat atgatttaaa aaaagcagaa
    19321 gaaaggtatc acattcttct tggactaatt attgctcttg ataatcttga taatgttata
    19381 aatttaataa gaagttcaaa aaat
//
LOCUS       DAOSAT010000022        18610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2076_length_18610_cov_4.611425, whole genome shotgun sequence.
ACCESSION   DAOSAT010000022 DAOSAT010000000
VERSION     DAOSAT010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18610
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2076_length_18610_cov_4.611425"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLF90_03110"
     CDS             complement(<1..513)
                     /locus_tag="PLF90_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOK56307.1"
                     /translation="MKRRVVITGIGVIAPNGIGKENFWKALTGGKSGIKKITSFDASE
                     LPVQIAGEVTDFKPEDYIEDRKKLRRMARFSQFAVACAKMAIEDSGLNLKEIDSEKIL
                     VCLGVSTSAMDLIESQYRKFLNKGYRGIVPFGIFSATPNMACQEILNEFLLEVPISTV
                     ASGCAAGLEAI"
     gene            complement(510..1682)
                     /locus_tag="PLF90_03115"
     CDS             complement(510..1682)
                     /locus_tag="PLF90_03115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOK56308.1"
                     /translation="MDIFEHLIFERGDFFDALCYGEGEETIIELSKFVQGKRKLEGIP
                     NIIYKENGKIIKTEREYIKSLDSLPFPEYSPNVYWKINEKIKMFVIDESRGCQNACYF
                     CIHPKKSGKRKKKSVDRLIEEIKFYINNYKVYLYRFAGSSTPGDLVLEFAKKVINEKI
                     KIDYANSGYVEDFDIDFEILKTSGCQSIFFGVESGNEEILKKGMNKNVKKSDMERVLK
                     RCKEAGIFTVASLIYPAPFETEETRKETIELLKEVKPDSALIQFPGIYPGTMWFNHPE
                     RFNFELIDKEKYPLNVMNYKIKALFPPSMWQPLPYKVNGMNFKKFAYETEKFQKDVLN
                     LGINTSISNEDYLFYLYSGFNTPEEFLRFNRVYFYSGNSEKLREEIERINKKSTDR"
     gene            complement(1734..2138)
                     /locus_tag="PLF90_03120"
     CDS             complement(1734..2138)
                     /locus_tag="PLF90_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56309.1"
                     /translation="MKGLLISIAGIPRSLSDFVPDNGLALLAACLLREKVEVNILDLN
                     LPEVFDEIYTKDIKKFLENFSYKVFIEGKKPSFIELIKLKKASSIIEENKKLYLEKLK
                     RFILEKVEKGKFDFMGFKLWAGDGFDWSIEIG"
     gene            2241..3410
                     /locus_tag="PLF90_03125"
     CDS             2241..3410
                     /locus_tag="PLF90_03125"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOK56310.1"
                     /translation="MENKKILVFFLICNSLLFSQAFKNPPESSSALSQSGAFVAQCDD
                     ASAINFNPAGLIQLRDGELIFGFNFPYFKTKYIYSSGEEEKKYKFSVLPYFYFVPETK
                     NDEFKFGIGFNTPYGQSTEWSQDIVRIWNYQVPYYSSMQTGNLMTAFSFKLTPELSLG
                     LGLNYYYSKLVFKNLIYIPVPGVETTAKINVDGSAFGGTIGILYKNDKWSTGIVYKSG
                     FRIDYKGTSVIFGDDYPSEIKINFPDRINFGVACYPLKNWKIEFDTEYYGFSSIKNVY
                     INPGFIPSYEIPKNWKNIYNFYLGTEYKKNDNLKLRGGIARLNSPIPDETWEPSLPDS
                     DTLIISFGTELGIKTGKIEMTLLTSIPKKIKKEGNYEGIYKSKGFFFTIGYKREI"
     gene            3349..4509
                     /locus_tag="PLF90_03130"
     CDS             3349..4509
                     /locus_tag="PLF90_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK56311.1"
                     /translation="MKEFINQKDFSLQLDIKEKFNENRDTILVIDDEMGPRESLIILF
                     KYKYNVITAEDGDKGIEILKNKKVDLVILDLRMPGKSGIETLAEIRKFNQNVPVIILT
                     GYGDMETARKAMHYEAIEFMSKPFSVSEMEEIVEKGIQKGKIKIETEKLKEELNLLKE
                     KLKKRINEIEALAIIGQTSSEILHEVNNLLTVIHGYIQLLIEEVNSKKITLKYLITID
                     NEIKRCKNIAKNILELAKEKSNIEDVNLNELIKNLIEFLRQSKLCQNINFLVKIPEYS
                     VIVKSNFNHLHQAILNIFLNSIQAIEKNGIINVLVEKKDNKGIIIIKDNGKGIPQDII
                     KKVIDPFFTTKETGTGLGLHLTTKIINKYGGQFEIISNEGIGTEFKIIFPLS"
     gene            complement(4501..5715)
                     /gene="rpoN"
                     /locus_tag="PLF90_03135"
     CDS             complement(4501..5715)
                     /gene="rpoN"
                     /locus_tag="PLF90_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOK56312.1"
                     /translation="MENKNVLNTKIYLKLLPSQILFSNILGFSTYSLIEFLKKESEEN
                     PFLKFEVDEEKLERISYEINIEEKLIEDLHLLNIPEKLMKIGEFIIMNLEDNGYFKMD
                     LKEVSEILNVKYEEVNETLKIIQTLEPYGIGARNLQECLLIQIKRLYKDNELFEIVQK
                     HWDLLTKRKYKEIALKMKINEKSVLLLLEKIKKLNPYPLTSSQKKLIKKIIPEGKITK
                     EGDSLKVYVEDKISPFIKIEVDYEKYLNSPFISPKEKKFLLKKMNRVKMIIEMLEKRR
                     KFLEDVFKEIVNYQKGYFESGNLLPLRMKDVAEKMNVNISIISRAVNGKYLISSKGLL
                     KIRDLFVPEFKYSISKTFIMEKIKKIIEDEKKPLSDKKISEKLGYFGIKISPRTVNKY
                     RNQMKILNSYLR"
     gene            5775..6572
                     /locus_tag="PLF90_03140"
     CDS             5775..6572
                     /locus_tag="PLF90_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOK56313.1"
                     /translation="MLNSILIVGCLGLFFGIFLTFIYTKFKIDENPLYSKVYELLPKG
                     NCGACGYAGCSAFAEVLIENKVTPEKCVMIGEKELSEICKLLGIEKIQKEKFVARICC
                     YGGTNAKKKFEYTTIKTCNVINSIFDTNLECVYGCLGFGDCVRICPVNAIEMKENGLP
                     EINEEKCIGCGKCVQICPKKIIKLLPYEKKVYVACSSLDKGATVIKICRSGCIGCGKC
                     AKACSENAIKIENNLAIIDYEKCNNCGKCAEECPRKIIFYIKISTLA"
     gene            6573..7328
                     /gene="truA"
                     /locus_tag="PLF90_03145"
     CDS             6573..7328
                     /gene="truA"
                     /locus_tag="PLF90_03145"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOK56314.1"
                     /translation="MEEKNFKLIISFKGKNYNGWQRQKNKTTIQEIIEKKCREIFKTD
                     VRIIGCGRTDSKVNGINYVANFKIKTKLTSLNLKNALNSKLPSDIYIKNVEEVSQDFH
                     SRYDAKKKTYRYIITFKKTPFLNDFAFYVKEKIDIEKIKKAIQYLTGEHDFKSFQSSG
                     SNVKNTIKKIFKISIKKVKFFIDEEVEILIIDIVGSGFLYKMVRNIVGALIHVGTGKI
                     EPEKIKEILEKKDRKFAPPPVPAYGLFFKNAEY"
     gene            7315..8220
                     /gene="murB"
                     /locus_tag="PLF90_03150"
     CDS             7315..8220
                     /gene="murB"
                     /locus_tag="PLF90_03150"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOK56315.1"
                     /translation="MQNINFESILENEFKGKFVKNFSLSQYTSFKCGGNVNYLIYPEK
                     IEEVKKIIEVSKKFGNNFIFLGKGTNVLVSDKGFDGIVISTLKMKNFKIEKNKIICEC
                     GLKISEILNICIKNSLTGFEFLAGIPGTIGGGIKNNAGLKTKWLSEKIRWIEYLDIEN
                     LNIIKKEKKDIFFDYRKSEFKNEFIWRSEFLLEKGKENEIRKTIKEYMKERMKKQPSG
                     YSAGSIFKNPYPYYAGELIEKCGLKGYKIGDCYISEKHANFIINKGKGKSQDVYELIK
                     IIKEKVMEKFNIKLELEIQLIGEFK"
     gene            8217..9137
                     /locus_tag="PLF90_03155"
     CDS             8217..9137
                     /locus_tag="PLF90_03155"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOK56316.1"
                     /translation="MKIAILYGGESPEREISLKSGRCIYFALRKKYKVKLFDPSKKNF
                     VNKFLKFKPDFVFIALHGGIGEDGSIQGFLETLKIPYSGSGVLTSAICLNKIICKEIL
                     IFNKIPTPPFIILEKNKKIKLPFKFPVVVKPANLGSTIGIKIVYSEKELENAVNDAFL
                     LDNEVFIEKFIKGKEVTVGIIGNENIEILPVIEIKVKKGFYDYKAKYTSGESFHIIPP
                     DLPEKIIKKIEKIAEKTYKVLKCSGFARMEIMVDYNYNIYVLEVNTIPGMTKLSLLPD
                     AAKFKGISFEQLCEKIINYGLEKWKKETKK"
     gene            9113..9880
                     /locus_tag="PLF90_03160"
     CDS             9113..9880
                     /locus_tag="PLF90_03160"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOK56317.1"
                     /translation="MEERNKKIEEIKKRILLKKRRKIKFFAFLFLIIFIICGFLFLKM
                     KIIKFLWNLEVFKIKEIKVYPETMTHIKDLLELEKDKNLLFLDVNNLIEKINSISEVE
                     KCKIIKNFPSAIEITIVLRKPWAILKYGEKEFLIDKNGVILNNEDRNIPDLIIYGIKV
                     NEKENKVEEIEKISILEELEKWYNYFNIGNFLKLNRIDISDINKIEISDGAKSIYFTH
                     ENIKIKMEKLSSVLKNLKEDYEYIDTRFKNFYIKLKK"
     gene            9877..11121
                     /gene="ftsA"
                     /locus_tag="PLF90_03165"
     CDS             9877..11121
                     /gene="ftsA"
                     /locus_tag="PLF90_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOK56318.1"
                     /translation="MKEQIITAVDIGTTKFFGLVGLVSETGIEVIASEVIKTEEDWIK
                     EGRIGYRDGVISGLVDLLDSLRKQSKEEIRWITVGVGGGHILGKIYSKKIEILPKGRP
                     INEGDVQLLEREIKNLISGESLEKRDIIYIVPQEFIIDDIPISIGRSPVGMHGNTLEM
                     RAHILTGESNPIKNISDCVKTAGVNLSSEIFPYSWAVAESVLSEEEKKSGCLVIDIGK
                     STTDIVLFSEGKIILTESIKVGSYHIDYDLSTKYHTSIEFAEELKKRYAWCDYKRFIG
                     EKKEEIKMVEILNPKGKSLGKVSTEEISKIAYWRVQYIFEDLILKNRLMKTGYFPTRV
                     SEVVISGGGAKLKGIVKLAEDIFQLPSRIGIPQKLLNLDKSYQKPEFSAGIGLLILAS
                     KVGKEDETIIDKIKKWFRRWLF"
     gene            11133..12215
                     /gene="ftsZ"
                     /locus_tag="PLF90_03170"
     CDS             11133..12215
                     /gene="ftsZ"
                     /locus_tag="PLF90_03170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOK56319.1"
                     /translation="MVKVIEESIVSSNINIKVVGIGNAGGNIVSRIYNKIDGVQFVIF
                     NTEAHALKNAKSDIKIQIGEKTTQGKGAGKDPEKGRFAASEDKEKIAEALKNTQIIFL
                     VAGLGGGTGTGGTPVIAKIAKDLGAITISLVTTPFEWEGEKRNQYAQEGLENILKNVD
                     TLIHISNQKLYEIIDEKASFEEAFWKIDEIILRTVSSISDLIYKPKLLDIDFADIYSV
                     VENAGRGIVGLGYGKGEGRMEQAVRSTIENPLIEKSEIMEAKNILISITGSPDLTLKE
                     VGEAVNIIQSRISSPSRVLGVATDQNLNDEIKITLLATGIGKLLSTKEKPVSEKKKIE
                     ELPLEKEEVEDSIDEPIWKRKEKEKI"
     gene            12212..13021
                     /locus_tag="PLF90_03175"
     CDS             12212..13021
                     /locus_tag="PLF90_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK56320.1"
                     /translation="MKIGICNEVFKDWTVEEIFYYIKDIGYEGIEIAPFTLAENVSEI
                     ADEKIEEIKKLSEKTGIKIIGTHWLLVKPEGLSISSKDKELRKKTSEYLCKLVDFTSR
                     IGGEIMVFGSPKQRKINEGQTIEEVKNYLKEVIIPVLEKCFEKNVFLCIEPLARTETN
                     FINKAEEAIEIIEEINHPNLKLHLDVKAMTDEEKPIVEIIEIGSKYLKHFHVNDKNLL
                     GPGFGEIDYKPIIEKLKKVGYNGWLSVEVFDFTPGPKVIAEKSIKYLKKFL"
     gene            13043..14080
                     /locus_tag="PLF90_03180"
     CDS             13043..14080
                     /locus_tag="PLF90_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK56321.1"
                     /translation="MDKIKVGIAGLGRSGWDIHCKTISQLPNLYEIVAVCDLDEKRRA
                     EAEKTFKCVSYKNFDELIKDKSIELVIVATLSNMHTEHTIKALKNGKNVVCEKPMATN
                     LKDADKMIKVAKETGNLLTIFQNRRYSPDFLKVKEIIDSGIIGRVVLIKMLQHSFGRR
                     WDWQTLKKYGGGLLNNWGPHIIDQALQLFGDKEEPEIFCHMEKTLTLGDTEDHIKIIL
                     HGKVSPLIDIEITSCCCYPQENWLVMGTKGGIKGTTNKLIWKYFKSENLPERKVEELP
                     TPDRSYNREEIPWEGEQIWEPSEKDLNFQINFYIDLYQTIRNNKPLKITPESVRKVIW
                     VIEKCHKIANI"
     gene            14094..15209
                     /locus_tag="PLF90_03185"
     CDS             14094..15209
                     /locus_tag="PLF90_03185"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOK56322.1"
                     /translation="MKKIIINNDKRQWHNFPLKVEHSDDNLILVSKGRKFFAQIDEEH
                     NKKYLIFLPGEIEKGVEIEFNIEKIDSLPEIVKINDDKNGKVNVFINETLFTTYNYSQ
                     ENARPFLNPVIGPKGKQMVRKPASQGNPEKFDHIHHRGIWVAHGDVNGTDNWSEMEGH
                     GKTVHKKFLKLVSGPVFGIIHSLNDWVDNKGRKILEEERIIKIYNLPPESRIIDHIII
                     LRATETEVVFKDTKESGLLSIRVNPEMEGRHKGIIKNSYGGTGEIECWGKRSFWVDYS
                     GEIEGIKCGISVFDWPDNLRYPTYWHVRDYGLFSANFFGLSDFYENKKISGTYILPYG
                     EELKLFYRIYIHSGNCEESNIGEKYLNFLYPPRVEIK"
     gene            15206..16270
                     /locus_tag="PLF90_03190"
     CDS             15206..16270
                     /locus_tag="PLF90_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK56323.1"
                     /translation="MSVKERILNTFNHCKIDRISFLTYPFLIPHGEIERLLRNNGFGY
                     WWITSPLIIKNEKIERVTTNFYKNGKEFIKVSLKCKHGEIFEIWETGGGYGTSLRKKF
                     FIEREEDYYIFEEIAKTEKIDVDKELYKNIEKNLGNDGILVSWLPKTPFQSILYELIG
                     PENYAFHLFDYQNLFLNLYEFLFKRYLKKCEILSETEFEFFEIADNITSEMIGLERFK
                     KFILPVYEKVCEIFHYKNKKIGSHMDGNLKILVEEIHKTKLDFIDAFTPYPDTDLTLK
                     EAKEKWKEKIIIINYPSSVHIRKEEEIKKITQILIEEAYPGDNFIINLTENIPSEHWQ
                     KSLKIINETLIEYGEVPKGG"
     gene            16273..17802
                     /gene="xylB"
                     /locus_tag="PLF90_03195"
     CDS             16273..17802
                     /gene="xylB"
                     /locus_tag="PLF90_03195"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOK56324.1"
                     /translation="MEYLMGIDIGTTGVKIVVIDIEGNLKGTHTVEYPLITPRPGWAE
                     QNPNDWWNATIKCIKEIIEKLKIKGEEIKGIGLTGQMHGSVFLDINYNVIYPAILWCD
                     QRTYEECNDINEIVGKDRIFEITCNPVLTGFQAPKILWLRKNKPEIYKKVRKILLPKD
                     YIRFCLISEFATDVSDASGTSLFDVRNRKWSEEILEKLNIPEDFLPKVYEGTEITGYI
                     KKDVAKLTGLFEKTPVIAGGGDQAAGGVGNGIVEEGNVSVTIGTSGVVFAHSEKVIVD
                     PKGRLHTFCHAVPGKWHLMGVMLSAGGSFRWLRDNLCEEEKKEGKRKKKDPYEIMTEK
                     AKSIPIGSEGLIFLPYLTGERCPYPDPNARGVFFGLSLKHTKAHIIRSVMEGITFGLR
                     DSIEIMKEINLPLGEKFIASGGGGKSDFWCQMIADIFGKNIVRLTSQEGAPFGAAILA
                     GVGTGIYKNVKSACKTILKEKDLFRFETNNFKTYEKFYRIYKELYQKLKNSFDNLAKI
                     L"
     gene            17841..18236
                     /gene="rpsI"
                     /locus_tag="PLF90_03200"
     CDS             17841..18236
                     /gene="rpsI"
                     /locus_tag="PLF90_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOK56325.1"
                     /translation="MDNKQLLIGVGRRKKSVAVVKMKEGEGKIIINKKTLEQYFPLFY
                     QQEDAIKPLIVTNLKGKFDFEIKVEGGGITGQVDAIKLGIARALVKSDPSLTPKLKEY
                     GLLTRDPRMKERKKYGQKGARKKFQWTKR"
     gene            18238..>18610
                     /locus_tag="PLF90_03205"
     CDS             18238..>18610
                     /locus_tag="PLF90_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK56326.1"
                     /translation="MIKKLNVKNFKSLKELEITCKRINVFIGEPNSGKSNILESLGFL
                     SFLGGYAIELKNFVRMLQIIDLFYDLNIREKIFIELDEKKIIAEYRNGNLFLNISPSP
                     KEKNINLNLYPPFTHHKISPEV"
BASE COUNT         7458 a         2218 c         3016 g         5918 t
ORIGIN      
        1 aatcgcttct aatccagcgg cacatccact tgcaacagtt gatataggaa cctctaataa
       61 aaactcattt aaaatttcct gacaggccat atttggagtc gctgaaaaaa tcccaaaagg
      121 aacaattcct ctataccctt tgtttaaaaa ttttctatac tgtgattcta tcaaatccat
      181 agcacttgta ctgacaccaa gacataccaa tattttctcg gagtcaattt cttttaaatt
      241 caatccgcta tcttcaattg ccattttagc acaggcaacc gcgaattgtg aaaatcttgc
      301 catcctcctt aatttctttc tgtcctctat ataatcctct ggcttaaaat cagttacttc
      361 accagcaatt tgaactggta attctgaagc atcaaatgaa gttatctttt ttattccgct
      421 tttccctcct gttaatgcct tccagaaatt ctccttccct atgccgttag gagcaataac
      481 accaattcca gtaataacaa ctcttctttt catctgtctg tacttttttt atttattctt
      541 tcaatttctt ctcttagttt ttcactattt ccagaataaa aataaactct gttaaatctt
      601 aaaaattctt ctggtgtatt aaatccactg tataaataaa aaagataatc ttcatttgaa
      661 attgatgtat ttattccaag gtttaaaaca tctttctgaa atttttctgt ctcatatgca
      721 aattttttaa aattcatccc gttaactttg taaggtaatg gttgccacat acttggtgga
      781 aaaagtgctt ttatcttata gttcattaca tttaaaggat acttttcttt atctatcaat
      841 tcaaaattaa atctttcagg atgattaaac cacattgttc ctggataaat acctggaaat
      901 tgaatcaggg cagaatctgg tttaacttct ttaagaagtt caattgtttc ttttctggtt
      961 tcttctgttt caaaaggtgc tggataaatt aaacttgcaa cagtaaaaat ccctgcttct
     1021 ttacatcttt tcagtaccct ttccatatct gatttcttaa catttttatt catacctttc
     1081 tttaaaatct cttcattacc tgattcaaca ccaaaaaata ttgattgaca acccgatgtt
     1141 ttcaaaatct caaaatcaat atcaaaatcc tccacatatc cagaatttgc gtagtcaatt
     1201 ttgatctttt cattaataac tttttttgca aattctaaaa ctaaatctcc aggtgtactt
     1261 gaacctgcaa atctataaag ataaacttta taattattta tataaaactt aatttcctca
     1321 atcaatctat caactgactt cttcttcctt tttccacttt tttttggatg tatacaaaaa
     1381 tagcaggcat tttgacaacc tctactttca tcaattacaa acatttttat tttttcattt
     1441 attttccaat aaacatttgg actatattca ggaaagggta aactatcaag cgatttaata
     1501 tattctcttt ctgtttttat aatctttcca ttttccttat aaattatgtt tggaattcct
     1561 tccaattttc ttttcccttg tacaaatttt gacagttcaa taattgtttc ttctccttct
     1621 ccataacaaa gtgcatcaaa aaagtcacct ctttcaaaaa ttaaatgttc aaaaatgtcc
     1681 acctgaggtc caccaccgaa tatttttatt tctggctttt tcttttttaa aaatcatcca
     1741 atttctattg accaatcaaa cccatctcca gcccatagtt taaatcccat aaaatcaaat
     1801 tttccctttt caactttttc aagaataaac ctttttagtt tttcaagata taatttttta
     1861 ttttcttcaa taattgaact ggctttttta agttttatta attcaataaa tgacggtttt
     1921 ttcccttcta taaatacttt ataagagaaa ttttcgagaa atttttttat atcttttgta
     1981 taaatttcat caaaaacttc tggtaaattt aaatcaagta tattaacttc tactttttct
     2041 ctaagaagac aagcggcaag taaagcaaga ccattatcag gaacaaaatc agacaaactt
     2101 ctcggtatac ctgcaataga aattaataaa cctttcataa cttatattgt acaactttgt
     2161 ccagagtaat tcaaattatt ttttacatta acttggcata ctttttgcat ataagtgagg
     2221 cataaaaaag gaggttattt atggaaaaca aaaaaatttt agtttttttc ttaatatgca
     2281 attctttgtt attttctcag gcatttaaga atccacctga aagttcctct gctctttctc
     2341 aaagtggtgc gtttgtagca caatgtgatg atgcttccgc aattaatttc aatccagcag
     2401 gtcttattca attaagagat ggagaattaa tttttggttt caattttcca tattttaaaa
     2461 ctaaatatat atactcaagc ggagaagaag aaaaaaaata taaattttca gttctacctt
     2521 atttttattt tgttccagaa acaaaaaatg atgaatttaa gtttggaata gggttcaata
     2581 ctccatacgg acaatcaaca gagtggagcc aagatattgt caggatatgg aattatcaag
     2641 ttccttatta ctcaagtatg caaacaggta atttaatgac tgcattttct tttaaattaa
     2701 caccagaact ttctttggga ttaggattaa actattatta tagcaaatta gtttttaaaa
     2761 atcttatcta tattccagtt cctggagttg aaaccacagc aaaaataaat gttgatggaa
     2821 gtgcttttgg agggactata ggaatcttat acaaaaatga taaatggtcc acaggaatag
     2881 tttataaatc tggatttaga atagattata aaggaacttc tgttattttt ggagatgatt
     2941 atccatcaga aataaaaata aattttcctg atagaataaa tttcggtgta gcctgctatc
     3001 cattaaaaaa ctggaaaatt gaatttgata cagaatatta tggattctct tccattaaaa
     3061 atgtctatat aaatccaggt ttcattcctt catatgaaat accaaaaaac tggaaaaata
     3121 tttataattt ttatcttgga acagaatata aaaaaaatga taatctgaaa ttaagaggtg
     3181 gtatagcaag attaaattct cctattcccg atgaaacatg ggaaccaagt ttaccggatt
     3241 cagatacgtt aataatttct tttggtactg aattaggaat caaaacagga aaaatagaaa
     3301 tgactttatt aacttcaata cccaaaaaaa taaaaaagga gggaaattat gaaggaattt
     3361 ataaatcaaa aggatttttc tttacaattg gatataaaag agaaatttaa tgaaaacagg
     3421 gatactattc ttgttataga tgatgaaatg ggaccgagag aatctctcat aatacttttt
     3481 aaatacaaat ataatgttat tacggcggaa gacggagata aaggtataga aatattaaaa
     3541 aataaaaaag ttgaccttgt tatccttgat ttaagaatgc cgggtaaaag tggcattgaa
     3601 acacttgcag aaataaggaa attcaatcaa aatgtacctg taattatttt aacaggttat
     3661 ggagatatgg aaactgcaag aaaagcaatg cattatgaag caattgaatt tatgagcaaa
     3721 ccattttctg tttccgaaat ggaagaaatt gtagaaaagg ggatacaaaa agggaaaatc
     3781 aaaattgaga ctgaaaaatt aaaggaagaa ttaaatttat taaaagaaaa actaaaaaaa
     3841 agaattaatg aaattgaagc acttgcaata attggacaaa catcatcgga aattcttcac
     3901 gaggtgaata atcttcttac agtgattcat ggttatattc aacttttaat agaagaagtt
     3961 aactcaaaaa aaatcacatt aaaatattta ataaccattg ataatgaaat aaaacgatgt
     4021 aaaaatattg ctaaaaatat tcttgaactt gcaaaagaaa aatcaaatat agaagatgta
     4081 aatttaaatg aattaataaa aaatctgatt gaatttttaa ggcaatcaaa attatgccag
     4141 aatattaatt ttttagttaa aattccagaa tattctgtta ttgttaaaag caattttaat
     4201 catttacatc aggcaatttt aaatattttt ttaaatagta ttcaggcaat tgaaaaaaat
     4261 ggaattataa atgttttggt tgaaaaaaaa gataataaag gaataattat aataaaggat
     4321 aatggaaaag gtatacctca ggatattatt aaaaaagtta tagacccttt tttcacaaca
     4381 aaggaaacag gaacaggttt aggattgcat ctaacaacaa aaattataaa taaatatggt
     4441 ggtcaatttg aaattataag taatgaaggc atagggacag aatttaaaat aatatttcca
     4501 ttatcttaaa taagaattaa gaattttcat ttgatttcta tatttattaa cagtgcgagg
     4561 tgatattttt ataccaaaat atccaagttt ttcagatatt tttttatcag aaagtggctt
     4621 tttttcatcc tcaattatct tttttatttt ttccattata aaagttttac ttattgaata
     4681 tttaaattca gggacaaata aatctctaat ctttaaaagc ccttttgaag aaatgagata
     4741 tttcccattt accgctctgc ttattataga tatattaaca ttcatttttt cagcaacatc
     4801 tttcattctt aaaggtaata aattcccgct ttcaaaataa cctttctgat aatttacaat
     4861 ctctttaaaa acatcttcaa gaaattttct tcttttttca agcatttcta taatcatttt
     4921 tactctattc atttttttca aaagaaattt tttttctttc ggtgaaataa aaggtgaatt
     4981 tagatatttt tcatagtcca cttcaatttt tataaaagga gaaattttat cttcaacata
     5041 tacttttaag gaatcccctt ctttcgttat tttaccttca ggaataattt tttttataag
     5101 ttttttttgc gaacttgtaa gaggataagg atttaatttt ttaatctttt ccagaagaag
     5161 taaaacagat ttctcattta ttttcatttt tagtgctatc tctttatatt ttctcttagt
     5221 aagcaaatcc cagtgttttt gaacaatctc aaaaagttca ttatctttat ataatctttt
     5281 aatttgaatt aaaagacatt cctgtaaatt tcttgctcct atcccatagg gttccagcgt
     5341 ttgaataatt tttaatgtct cattaacttc ttcatatttt acatttaaaa tttctgagac
     5401 ctcttttaaa tccattttga agtagccatt atcttcaaga ttcattataa taaattctcc
     5461 tattttcatt aatttttctg gaatatttaa aagatgtaaa tcttcaatca gtttttcttc
     5521 aatattaatt tcataagata tccgctccaa tttttcttca tcaacctcaa atttcaaaaa
     5581 tggattttct tcactttctt tttttaaaaa ttcaataaga gaataagtag aaaatcccag
     5641 aatatttgaa aaaagtattt gtgaaggtaa gagttttaga tatatttttg tattcaaaac
     5701 atttttattt tccatattta aattttaact catatttgca aaatgttgta taatatgatt
     5761 taaaaggagt aaaaatgtta aattctatat taattgtggg ttgtctcggt ttattttttg
     5821 ggatttttct tacatttatc tatacaaaat ttaagattga tgaaaaccct ttatattcta
     5881 aggtttatga acttttaccg aaaggaaatt gtggtgcctg tggatatgca ggatgtagtg
     5941 cgtttgcaga agtattgatt gaaaataaag taacacctga aaaatgtgtt atgataggag
     6001 aaaaagaact atcagaaatt tgtaaactac tgggtattga gaagattcaa aaagaaaaat
     6061 ttgtagcaag aatatgttgt tatggaggaa caaatgcaaa aaagaaattt gaatatacta
     6121 caataaaaac atgtaatgtt ataaattcaa tttttgatac aaatcttgaa tgtgtctatg
     6181 gctgtcttgg atttggtgat tgtgttagaa tctgtcctgt taatgccata gaaatgaaag
     6241 aaaatggact acctgaaata aatgaagaaa agtgtatagg atgtggaaaa tgtgtccaga
     6301 tatgtcctaa aaagataata aaattattgc cctatgagaa aaaagtgtat gttgcctgtt
     6361 cttctctgga taaaggtgct acagtaatta aaatttgcag gtctggttgt ataggttgtg
     6421 gtaaatgtgc taaagcgtgt tctgaaaatg cgataaaaat agaaaataac cttgctatta
     6481 ttgattatga aaaatgtaat aattgtggga aatgtgcaga agagtgtccc aggaaaataa
     6541 tattttatat aaaaatttca actcttgcgt aaatggaaga aaaaaatttt aaacttataa
     6601 tttcttttaa aggtaaaaat tataatggat ggcagagaca aaaaaataaa accacaatac
     6661 aggaaattat tgaaaaaaaa tgcagggaaa tttttaaaac agatgttaga attatcggat
     6721 gtggaagaac tgatagtaaa gttaatggaa taaactatgt ggcaaatttc aagataaaaa
     6781 caaaattaac ttctttaaat ttaaaaaatg ctttaaattc taaacttcca tctgatatct
     6841 atattaaaaa tgttgaggaa gtcagtcagg atttccatag cagatacgat gcaaagaaaa
     6901 aaacttatag atatataata acttttaaaa aaactccttt tcttaatgat tttgcatttt
     6961 atgtaaaaga aaaaatagac attgaaaaaa taaaaaaagc aattcagtat cttacaggag
     7021 agcatgattt taaatcattt cagagttcag gaagtaatgt taaaaataca ataaaaaaaa
     7081 tattcaaaat cagtataaaa aaggttaaat ttttcattga tgaagaagtt gaaatattaa
     7141 taatagatat agttggaagt ggttttttat ataaaatggt tagaaatata gttggtgctc
     7201 tcattcatgt tggaacagga aaaattgaac cggaaaaaat aaaggaaatt cttgaaaaaa
     7261 aagatagaaa atttgcacca ccaccagttc cagcatacgg actatttttc aaaaatgcag
     7321 aatattaatt ttgaatctat tttagaaaat gaatttaaag gaaagtttgt aaaaaatttt
     7381 tctctaagtc aatatacaag ttttaaatgc ggtggtaatg taaattatct gatataccct
     7441 gaaaagattg aggaagtaaa aaaaattatt gaagtttcaa aaaaatttgg taataatttt
     7501 atctttttag gaaaaggaac aaatgtactt gtaagtgata aaggatttga tggaatagtg
     7561 atttctacct taaaaatgaa aaactttaaa attgagaaaa acaaaattat ttgtgaatgt
     7621 ggactaaaaa tttctgaaat tttgaatata tgtattaaaa attccttaac tggttttgag
     7681 tttttggcag gtatccccgg aactatcgga ggtggaataa aaaataatgc aggtctgaaa
     7741 acaaaatggt tatcagaaaa aatccgttgg attgaatatc ttgacattga aaatttaaat
     7801 ataataaaaa aagaaaaaaa agatatattt tttgattaca gaaaaagtga atttaaaaat
     7861 gaatttatat ggagaagcga atttttactt gaaaaaggga aggaaaatga aatcagaaaa
     7921 acaataaagg aatatatgaa agaaagaatg aaaaaacaac cctcaggata cagtgcaggt
     7981 agtatattta aaaacccata tccttattat gcaggcgaat taattgaaaa atgtggatta
     8041 aaaggatata aaattggaga ttgttatatt tctgaaaaac atgcaaattt tataataaac
     8101 aaaggaaaag ggaaatctca ggatgtatat gaattgataa aaataataaa agaaaaagta
     8161 atggaaaaat ttaatataaa attggaactg gaaatccaac ttataggaga attcaaatga
     8221 aaattgcgat tttatatgga ggcgaaagtc ctgaaagaga aatatcatta aaatccggga
     8281 gatgtatcta ttttgcctta agaaaaaaat ataaagttaa attatttgac ccatctaaaa
     8341 aaaattttgt aaataaattc ttaaaattta aacctgactt tgtatttatt gctttacatg
     8401 gggggattgg cgaagatgga agtattcagg gatttcttga aactctcaaa ataccttatt
     8461 caggttcagg agttttaacc tctgctatat gcttgaataa aataatatgt aaagaaatat
     8521 taatattcaa taaaattcca actcctccgt ttataatcct tgaaaagaat aaaaaaataa
     8581 aattaccttt taaatttcca gttgtagtta aaccagcaaa tctcggttca acaattggga
     8641 taaaaatagt ttattcagaa aaagaacttg aaaatgctgt aaatgatgcc tttttactgg
     8701 ataatgaggt ttttattgaa aaatttataa aagggaaaga agttacagta gggataatag
     8761 gaaacgaaaa tattgaaatt cttccagtta tagaaattaa agtaaaaaaa ggattttacg
     8821 attataaagc aaaatatact tcaggtgaaa gttttcacat cattccacct gatttaccgg
     8881 aaaaaattat aaaaaagatt gaaaaaatag cagaaaaaac atataaagta ttgaaatgta
     8941 gtggttttgc aagaatggaa ataatggtag attacaatta taatatctat gttcttgaag
     9001 taaacactat tccgggtatg accaaattaa gtttacttcc agatgctgca aaatttaagg
     9061 gcatcagttt tgagcaactt tgtgaaaaaa ttataaatta tggactggaa aaatggaaga
     9121 aagaaacaaa aaaatagaag aaataaagaa aagaatttta ttgaaaaagc gaagaaaaat
     9181 taagtttttt gcatttttat ttttaataat atttataatc tgcggctttc tctttttaaa
     9241 aatgaaaata ataaaatttt tgtggaatct ggaagtgttt aaaatcaaag aaataaaagt
     9301 ctatccggaa acgatgacac atattaaaga tttattggaa cttgaaaaag ataaaaattt
     9361 gctgtttctt gatgttaata atttaattga aaaaattaat tctatatcag aagttgaaaa
     9421 atgtaagata ataaaaaatt ttccttctgc gattgaaatt acaattgtct taagaaaacc
     9481 atgggcaatt ttaaaatatg gagaaaaaga atttcttatt gataaaaatg gagttatact
     9541 taataatgaa gacagaaaca tacctgattt gattatttat ggaataaagg taaatgagaa
     9601 ggaaaataaa gttgaagaaa ttgaaaaaat tagtatttta gaagaacttg aaaaatggta
     9661 taattacttc aatattggaa attttttaaa attaaacaga attgatatat ctgatataaa
     9721 taaaattgaa ataagtgacg gagcaaaaag tatttatttt acacatgaaa atattaaaat
     9781 aaaaatggaa aaactttcat ctgttctcaa gaatttgaaa gaagattatg aatatattga
     9841 tacaagattc aaaaattttt atataaaatt aaaaaaatga aagaacaaat aataactgca
     9901 gttgacatag gtacaacaaa attttttggt cttgttggac ttgtaagtga aacagggatt
     9961 gaggttattg caagcgaagt tataaaaaca gaagaagact ggattaaaga aggacgaata
    10021 ggatacagag atggagttat aagtggtctg gttgatttac ttgactcttt aagaaaacaa
    10081 tcaaaagaag aaataaggtg gataacagtt ggagtgggag gtgggcatat attgggtaaa
    10141 atttattcaa aaaaaataga aattttgccc aaaggaagac caataaacga aggggatgtt
    10201 caattacttg aaagagaaat caaaaattta atatcaggag agagtttaga aaaaagggat
    10261 ataatttata tagtccctca agaatttata atagatgata ttccaatatc tattggaagg
    10321 tcacctgttg gaatgcatgg gaatacactt gaaatgagag cacatatact tacaggtgaa
    10381 tcaaacccta taaaaaatat tagtgactgt gtaaaaacgg ctggagtaaa tttatcatca
    10441 gaaatatttc cttattcatg ggcagtagca gagtcagtcc tgagtgaaga ggaaaaaaaa
    10501 tcaggttgtt tggtaattga tattgggaaa agcaccactg atatcgttct tttcagcgaa
    10561 gggaaaataa ttcttacaga atctataaaa gttggttcat atcatattga ttatgatctt
    10621 tcaacaaaat atcacacatc tattgaattt gcagaagaat taaaaaaaag atatgcatgg
    10681 tgtgactaca aaagatttat aggagaaaaa aaagaagaaa taaaaatggt ggaaatttta
    10741 aatccaaaag gtaaatctct tggaaaagtc agtactgaag aaatatcaaa aattgcatac
    10801 tggagggttc aatacatttt tgaggattta atattaaaaa acagacttat gaaaacagga
    10861 tattttccaa caagagtttc tgaagttgta atttcaggtg gtggagcgaa gttaaaaggt
    10921 attgtaaaat tagcagagga catttttcag ttaccttcaa gaattggtat acctcaaaaa
    10981 ttattaaatc ttgacaaatc ttatcagaaa cccgaatttt cagcaggtat aggcctttta
    11041 attcttgctt caaaagtagg aaaagaagat gaaactataa ttgataaaat taaaaaatgg
    11101 tttagaaggt ggctctttta aagggggtta aaatggtaaa agttatagaa gaatcaattg
    11161 tttcttcaaa catcaatata aaggtggtag gaataggaaa tgctggtgga aatattgtat
    11221 caagaattta taacaaaatt gacggggtac aatttgtaat tttcaatact gaggcacatg
    11281 ctttgaaaaa tgcaaaatct gatataaaga tacagatagg agaaaaaact acacagggaa
    11341 aaggagctgg aaaagaccct gaaaagggaa gatttgcagc aagtgaagat aaagaaaaaa
    11401 tagcagaagc attaaaaaat actcagatta tttttttggt tgcaggactt ggtggtggga
    11461 ctggaacagg aggaacgcct gtaatagcta aaatagcaaa agaccttggt gcaatcacaa
    11521 tttcgcttgt tacaactcca tttgaatggg aaggtgaaaa aagaaatcaa tatgctcaag
    11581 aagggcttga aaatatatta aaaaatgttg atactttgat acatatatca aatcaaaaac
    11641 tatatgaaat aatagatgaa aaagcttctt ttgaagaagc attctggaaa attgatgaga
    11701 ttattttaag aacagtttct tcaatttcag accttattta taaaccaaaa ttacttgata
    11761 tagattttgc agatatttat tctgttgtag aaaatgcagg tagaggtata gtcgggcttg
    11821 gatatggaaa aggagaagga agaatggaac aggcggtcag aagcacaatt gaaaatccgt
    11881 taatagaaaa aagtgaaata atggaagcaa aaaatattct tatcagtatc acaggaagtc
    11941 ctgatttaac attaaaagaa gtaggagaag cagtaaatat aattcaatca agaatttcaa
    12001 gtccttcaag agttcttgga gtagcaactg atcaaaatct caacgatgaa attaaaataa
    12061 ctcttcttgc tacaggtata ggaaaattgt tatctacgaa agaaaaacct gtttcagaaa
    12121 agaaaaaaat tgaagagtta ccattagaaa aagaagaagt tgaagattct atagatgaac
    12181 ctatctggaa aagaaaagaa aaggagaaaa tatgaaaata ggaatctgta atgaagtttt
    12241 taaagattgg actgttgaag aaattttcta ctatattaaa gacattggat atgaaggaat
    12301 agagattgca ccatttactc ttgctgaaaa tgtttcagaa attgcagatg aaaaaataga
    12361 agaaattaag aaactttcag aaaaaactgg gataaaaata attggcacac actggctact
    12421 ggttaaacca gaaggattat caatatcatc aaaagataaa gaattaagga aaaaaacttc
    12481 tgaatacttg tgtaaacttg ttgattttac ttcacgaatt ggtggtgaaa taatggtatt
    12541 tggatcacca aaacagagaa aaataaatga agggcaaaca attgaagaag ttaagaacta
    12601 tttaaaagaa gtaattatcc ctgttcttga aaaatgcttt gaaaaaaatg tttttttatg
    12661 tattgaaccc cttgcaagaa ctgaaacaaa ctttataaat aaagcagaag aagcaattga
    12721 aataatagaa gaaataaatc atccaaatct taaattacat cttgatgtaa aagcaatgac
    12781 agacgaagaa aaaccaattg ttgaaataat tgaaatagga tcaaaatatc ttaaacattt
    12841 tcatgttaat gacaaaaatt tacttggtcc aggatttggg gaaattgatt ataaaccaat
    12901 aattgaaaaa ttaaagaaag ttggatataa tggatggcta tcagttgaag tatttgattt
    12961 tactcctgga cctaaggtta tagctgaaaa aagtataaaa tacctgaaaa aatttttgta
    13021 atttacgaaa aaggagaaaa taatggataa aataaaagtg ggaatcgcag gacttggaag
    13081 aagtgggtgg gatattcact gtaaaacaat ttcacaatta cctaacctgt atgaaattgt
    13141 agctgtatgt gatttggatg aaaaaaggag agcagaagca gaaaaaactt ttaaatgtgt
    13201 tagttataaa aattttgatg aattaataaa ggacaaatct atagaacttg ttatagtagc
    13261 cacattatca aatatgcata ctgaacatac aataaaagca cttaaaaatg gtaaaaatgt
    13321 tgtgtgtgaa aaacctatgg caacaaatct aaaggatgca gataaaatga taaaagttgc
    13381 aaaagaaaca ggaaatttat tgacaatttt tcaaaataga agatattcac cagatttttt
    13441 aaaagttaaa gaaattattg attcaggtat tataggtaga gttgttctaa tcaaaatgct
    13501 acagcacagt tttggaagaa gatgggactg gcaaacatta aaaaaatatg gaggtggatt
    13561 attaaataat tggggacctc acataataga tcaggcatta caattatttg gtgacaaaga
    13621 agaacctgaa attttctgtc atatggaaaa aacattaact cttggtgaca cagaagacca
    13681 tataaaaata atactccatg ggaaggtttc tcctttaata gacatcgaaa tcacatcctg
    13741 ctgttgttat ccacaggaaa attggcttgt aatgggaaca aaaggaggaa ttaaaggaac
    13801 cacaaataaa ttaatctgga aatactttaa atctgaaaat ttacctgaga gaaaagtaga
    13861 agaattgccc acacctgata gaagttataa cagagaagaa ataccatggg aaggtgaaca
    13921 gatatgggaa ccgtcagaaa aagatttaaa ctttcaaatt aatttttata ttgaccttta
    13981 ccaaacaata agaaataata aaccattaaa aattacccct gaatctgtaa gaaaagtaat
    14041 ctgggtaata gaaaaatgtc ataaaattgc aaatatttga aaggaggaga taaatgaaaa
    14101 aaattataat taacaatgac aaaaggcaat ggcataattt tccgttaaaa gttgaacaca
    14161 gcgatgataa tttaatatta gtttcaaaag gaagaaaatt ttttgcacag atagatgaag
    14221 aacacaataa aaaatatttg attttccttc caggagaaat agaaaaagga gttgaaatag
    14281 aatttaatat tgaaaaaata gattccttac ccgaaattgt aaagattaac gatgataaga
    14341 atggcaaagt taatgttttt ataaatgaaa cactatttac cacatataat tattctcaag
    14401 aaaatgcaag accattttta aatccagtta tcggtccaaa aggaaaacaa atggtaagaa
    14461 aacccgcaag ccaaggaaat ccagagaaat ttgaccatat tcatcacaga ggaatatggg
    14521 ttgctcatgg agatgtaaat ggaactgata actggtctga aatggaagga catggaaaaa
    14581 cagttcataa aaaatttcta aaattggtaa gtggacctgt ttttggaatt attcattctc
    14641 ttaatgactg ggttgataat aaagggagaa aaatacttga agaggagaga attataaaaa
    14701 tatataattt gccacctgaa agtagaataa ttgaccatat tataatttta agagccactg
    14761 aaacagaagt tgtttttaaa gatacaaaag aaagtggact attgagtata agagtaaatc
    14821 cggaaatgga aggaagacat aaaggaatta taaaaaattc ttatggaggg acaggagaga
    14881 ttgaatgctg gggaaaaagg tctttctggg tagattattc aggagaaata gaaggaatta
    14941 aatgtggaat ttctgtcttt gactggccag ataatttaag atatccaact tactggcatg
    15001 taagagatta tggtctattt tcagcaaatt tctttggact ttcagacttt tatgaaaaca
    15061 aaaaaatttc aggaacatat attttaccct atggagaaga attaaaactt ttttacagaa
    15121 tttacataca ttctggtaat tgtgaagaaa gtaatattgg agaaaaatat ttaaatttcc
    15181 tctatccgcc acgggtagaa ataaaatgag tgtaaaggaa agaattttaa acactttcaa
    15241 ccactgcaaa atagatagaa tatcattttt aacctatcct tttttaattc ctcatggaga
    15301 aattgaaaga ttactaagaa ataatggttt tggatactgg tggataacct ctcctttaat
    15361 tataaaaaat gaaaaaatag agagagtaac aactaacttt tataagaacg gaaaagaatt
    15421 tataaaagtc agtttgaaat gtaaacatgg agagattttt gaaatttggg aaactggcgg
    15481 aggatatggt acttccctga gaaaaaaatt ttttatagaa agagaagaag attattatat
    15541 atttgaagaa attgcaaaaa cagaaaaaat tgatgtggat aaagaactct acaaaaacat
    15601 agaaaaaaat ctcggaaatg atggaatttt agtttcatgg cttccaaaaa caccttttca
    15661 atcaatactt tatgaactta taggtcctga aaattatgcc tttcaccttt ttgactacca
    15721 gaatttgttt ttaaatttat atgaattttt atttaaaaga tatttaaaaa aatgtgaaat
    15781 tctatctgaa acggaatttg aattttttga aattgcagat aatattacat ctgaaatgat
    15841 aggactggaa agattcaaaa agtttatatt acctgtatat gaaaaggtat gtgaaatctt
    15901 tcattataaa aataaaaaaa ttggttctca tatggatgga aatttaaaaa ttttagtaga
    15961 agaaattcat aaaacaaaac ttgattttat tgatgctttt actccatatc cggatacaga
    16021 tttaacttta aaagaagcaa aagaaaaatg gaaagaaaaa attattataa taaattaccc
    16081 atcatccgtt catataagaa aagaggaaga aattaagaaa attacacaga tattgattga
    16141 agaagcatat ccaggagata actttatcat aaatcttaca gaaaatatcc catctgaaca
    16201 ctggcagaaa agtttaaaaa ttataaacga aacacttata gaatatggag aagttccaaa
    16261 aggaggataa aaatggaata tttaatgggt atagacatag gaacaactgg agtaaaaatt
    16321 gttgttattg atatagaagg gaatttaaaa ggtactcata cagttgaata tcctttaatt
    16381 actcctagac ctggatgggc agaacaaaat ccaaatgatt ggtggaatgc aacaataaaa
    16441 tgtataaaag agattattga aaaattaaaa attaaaggag aggagattaa aggaattggt
    16501 ttaacaggtc agatgcatgg ttctgtattc cttgatataa attacaatgt tatttatcct
    16561 gcaattttgt ggtgtgacca gagaacatat gaagaatgta atgatataaa tgaaattgtt
    16621 ggaaaagata gaatttttga aataacctgc aatcctgttt taacaggttt tcaagcacct
    16681 aaaattttat ggttaagaaa aaataagcca gaaatttata aaaaggtaag aaagatattg
    16741 cttccaaagg attatataag attttgtttg ataagtgagt ttgcaaccga tgtttcagat
    16801 gcttctggaa catctctttt tgatgttaga aatagaaaat ggtcagaaga aatacttgaa
    16861 aaattaaata tcccagaaga ttttttaccc aaagtatatg aaggaacaga aattactgga
    16921 tatattaaaa aagatgtggc aaaattaaca ggactttttg aaaaaacacc tgtaattgca
    16981 ggtggtggag accaggcggc aggtggagtt ggaaatggaa ttgtagaaga gggaaatgtt
    17041 tctgtcacaa taggaacaag tggagttgta tttgcacatt cagaaaaagt tattgttgac
    17101 cctaagggaa gattacatac attctgtcat gctgttcctg gaaaatggca tttgatggga
    17161 gttatgcttt ctgcaggtgg ctcttttaga tggctaagag ataatctatg tgaggaagaa
    17221 aaaaaggaag gaaaaagaaa aaagaaagac ccttatgaaa taatgactga aaaagcaaaa
    17281 agtattccta ttggttctga aggacttata tttttaccat atttaacagg agaaagatgt
    17341 ccatatccgg acccaaatgc aagaggcgta ttcttcggac tttctttaaa acatacaaag
    17401 gcgcacataa taagaagtgt aatggaagga ataacttttg gattgagaga ttctatagaa
    17461 ataatgaaag aaataaatct accccttggt gaaaaattta tagcaagcgg tggaggtgga
    17521 aaaagtgatt tctggtgtca gatgatagcg gatatatttg gaaaaaatat cgtaagatta
    17581 acttctcagg aaggagcacc ttttggagca gcaattttag caggagtcgg tactggaata
    17641 tataagaatg taaaaagcgc ctgtaaaact atactgaaag agaaggattt attcaggttt
    17701 gaaacaaata attttaaaac atatgagaaa ttttacagaa tttataaaga actctatcag
    17761 aaactcaaaa attcttttga caatttagca aaaattttat aaaatatttt tcttaaaata
    17821 agaaaggagt gaaaataaaa atggataata aacaattact tattggagta ggaagaagaa
    17881 agaaaagtgt tgcagttgta aaaatgaaag aaggagaagg taaaattatt ataaataaaa
    17941 agacacttga acaatatttt ccacttttct atcaacagga agatgcaata aaaccattaa
    18001 ttgtaacgaa cttaaaagga aaatttgatt ttgaaataaa agtagaaggt ggaggaataa
    18061 caggacaagt tgatgcaatt aaacttggta ttgcaagggc tcttgtaaaa tctgacccat
    18121 cattaacccc aaagttgaaa gagtatggac ttctaacaag agacccgaga atgaaggaaa
    18181 gaaaaaaata cggacagaaa ggtgcgagaa agaaattcca gtggaccaaa agataagatg
    18241 ataaaaaaat taaatgttaa aaattttaaa tccttaaaag aattagaaat aacctgtaaa
    18301 agaataaatg tatttattgg tgaaccaaac tctggaaaat caaatatcct tgaaagttta
    18361 gggtttttat cttttttagg cggatatgct attgagttaa aaaattttgt tagaatgttg
    18421 cagataatag atttgtttta tgatttaaat atacgagaaa aaatttttat tgagttggat
    18481 gaaaaaaaga taattgctga atacagaaat ggtaatttat tcttaaatat ttccccttca
    18541 ccgaaagaaa aaaatataaa tttaaattta tatcctcctt ttactcacca taaaatttct
    18601 ccagaagtga
//
LOCUS       DAOSAT010000023        18260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2129_length_18260_cov_5.026257, whole genome shotgun sequence.
ACCESSION   DAOSAT010000023 DAOSAT010000000
VERSION     DAOSAT010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18260
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2129_length_18260_cov_5.026257"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1092
                     /locus_tag="PLF90_03210"
     CDS             94..1092
                     /locus_tag="PLF90_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOK56327.1"
                     /translation="MEKIKQKKEETLKKILISIGVLFLFFIFNLWQSGSFSQLNRIVK
                     NPKLQVFTIVSLAILIEGFPFILIGSLISGTIEVLVSEDKFKKILPQGKFSSAVFGSF
                     LGFLFPVCSCGEIPVSRRLIKKGIPTPGGISYLLATAIINPITLFSTYLAFSSLKMVF
                     GRFLIAFIVSVFVGILIAPEDKSEILKEYEEGHKHDENLSKFERIFIHSGNDFLIMGK
                     YLIIGATLASLFSTFTSREVFIAIGKNKVLSILSMQILSIILSLCSYADAFVASSFTY
                     LPDISKIIFMISGPMMSISLILVYFAAFKEKFVKKLLVSVFLTLFILGIIGSFLGG"
     gene            1096..1836
                     /locus_tag="PLF90_03215"
     CDS             1096..1836
                     /locus_tag="PLF90_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HOK56328.1"
                     /translation="MKIFKNRQFYEFLIIIFYILFFISLIFTGNFKIFVKNFYFPYIV
                     IGVLILVFLLTIEIKKMKNFKQLNVYDIFSFIIFLFPLILFILVRPTALPTYAALKRG
                     IQTEFLSKDILKSLQEKIETEGKYKKLTIKQLLALSKSKPEEIEEKDVSIEGMVYKGE
                     EKEKFTLIRFLITCCAADATPLGVEVEYKGIKEKSTGEWIPPEEKEEFKNEDWVNVKG
                     KVKIEEGKVKIIAEEVKKTNPPSDPYLY"
     gene            2018..2443
                     /locus_tag="PLF90_03220"
     CDS             2018..2443
                     /locus_tag="PLF90_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOK56329.1"
                     /translation="MEKYKKSLKKNKLKITPKRVAIINYFLKNDKYFTPFDIWKYMKN
                     NFKKIGLPTVYRNLAYFEKIGILTKIEGEENRFYYGICKEEKNIHHHHIVCISCHKVD
                     DFGICNFEEIKNDIEKKTGFIITEHQFFLKGLCKNCKRR"
     gene            2447..3109
                     /locus_tag="PLF90_03225"
     CDS             2447..3109
                     /locus_tag="PLF90_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56330.1"
                     /translation="MKKIKFIGDALSHHIPFSVLSVTIGLIFAGILTFFVEILNIENP
                     SEYFEELFHIFHPTHMLFSALATTTMFWKNEKKFWKAFIIGVIGSAGICGISDIFIPY
                     ISGFLLGAKMKLHICIIQHPQIVLPFLFLGVFMGYVAPETLEKSEGVIFSHSLHVFVS
                     ATASIFYLISFGLIDWVHRIGSVLIYMVLAVVIPCCTSDIVFPLLFISKKDKLSKYSH
                     IH"
     gene            complement(3132..4958)
                     /locus_tag="PLF90_03230"
     CDS             complement(3132..4958)
                     /locus_tag="PLF90_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56331.1"
                     /translation="MRKFIIFITLFILFANGFGANWYEKNKQLSVVFSNEVWKLVEKA
                     NYYSNRGNIKSADAYLRTAKEKTEKCEPFSPNNWPFGWPRDKESLKYLKYATPVAFIY
                     RIIGDFCLDTNYIKESIRYFEVYLSKCLIPDADYYMLLASIYEKEGMYNQAINLYYEI
                     SKFIENKNYWGKDYSISDIEKKIKNLNLMFKKNLVIILNPVFIDIPPFIQTDFSSILT
                     DEINNIKNVIIVPRESFLKVLNEQKFIEMSIEDEELSIAGKILNADYVFKPSLAKIVN
                     TYVFNLDVFSINKKKWFEHYEYKTDDMRYIPNMIKRFTVNFQGLDIPSSLFLPETKFL
                     WSYETDDLINDLKTSRDKKRILIGCDSGSAYLINDKGVLIRKLNMSEKIIKVSISPEG
                     NYFSFFTLEGILYFLTGNGKILWTKKTGNLGRGIGISENGKFIVAGIDKRILYIDKNG
                     EIFWDLNLPEIISFVNIKDDGSLVFTGTENGKLYCYRDDGNLKWEKNINGKIINIKPG
                     ENYICVETETGMIYLYDLNGNEIKNFRNDEEINYNIFSSEILELISGKKGNFLFFLSY
                     DKKSLWRYILKDRLNFLESLPDGKFIVSAEGKNIFSFSIIWK"
     gene            complement(4955..7609)
                     /locus_tag="PLF90_03235"
     CDS             complement(4955..7609)
                     /locus_tag="PLF90_03235"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOK56332.1"
                     /translation="MRKISKIFIFLYLLNFSIYGESQVLTFGTKTKKIEFLKKVLNNK
                     DKVYIKEVANFLDDEDKEVRTFASLILYEIGDSTCIDFYKKSLGDEYWQIRLYGIKGL
                     VKFGEGDILENLFASLDDPYWQVRYYAAIGIGKYGDENSISNLISHLNETNLEVKKAI
                     LISLKRLLWKNITRFNFKSMDENQLKLLFDCFESNDEQIKLLTISIFESVKDVRSIPY
                     LVKLLGDKSDEVKIKSLWALEGFKTKNIEEIEGLLNEPSVKVKIEAIKTLVRLKGEEG
                     INGLIKGLSDENESVKIYSLWALEKFKNPASYPEIVKCIADKSLSVREEAINIIERTD
                     DSIFIPILEKFIENKETNIEHKKLAIIELGKIGNLDLNRAKDILKKYLKSSDREIRYT
                     SIEGFYYLDKFDDYYIKNLVYMEKNDPDIRIRKCSSRYLGEIIDECIIKINSMRESDR
                     KFVIDKIDSFIGSNEINKLLIKMFYSKYPEVREKAVFVLKEMPRKVFAKNVKELAKES
                     DVELKKLCAIVLGEMRDLSSIGILKQGLTHFDPEYQLICANSLAKMGRNDGINVIMKN
                     INNDDVYYQKIAVESLIYLDKIAYSPIFIKKLHDSELDIKIISAWGLARLGNLTGLEV
                     LVRLSEINVEPIRTLANIYLKDQKIPSNLRSKIPFIREEIYRNKLGVQEVSPKIMYSY
                     KTDIPVEIDGKDDEGIWKMIEEINGFISIEDEKVFIDAQTKIASVYDNENIYFLIKCE
                     NPSNGVINFDTRDFLTISINPKNSFNEWYQFVFHPLKFTFPLSEEVRYLKYSYKWKFY
                     KNEEDVDKLWNSDWKIDTDVSSPGTVRKWIAEIAIPIKDLKVEKIIKGTQWTINFQRE
                     INNYIISTWTGRIDIPDQFGLIIFKESP"
     gene            complement(7593..8264)
                     /locus_tag="PLF90_03240"
     CDS             complement(7593..8264)
                     /locus_tag="PLF90_03240"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOK56333.1"
                     /translation="MKKIILTREEILKIEKETVEKFGISNLILMENAGRESFYVIKKI
                     LKDIKNRKFFILCGKGNNGGDGFVLTRYLFNNGANVKVFYLGKPEDFTDISFLNFEIL
                     RRLKIDTESINDLKLKGLKIERKDVFIDAILGIGIKGEIKGDMRNVIEFINKSSIYTV
                     SIDIPSGLDADTGEIHGVSVKSNITISMGFLKKGFFTGKGPEYVGKIKLVDIGFPKYY
                     YEKDK"
     gene            complement(8261..9640)
                     /gene="murC"
                     /locus_tag="PLF90_03245"
     CDS             complement(8261..9640)
                     /gene="murC"
                     /locus_tag="PLF90_03245"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOK56334.1"
                     /translation="MKGFIKNSKRIHLIGIGGIGVSGLAKILLRLNKKVSGSDNNYSP
                     IIKKLRKLGIKVYIGQNEKNIDSDIDLVIYSQAILPDNPEYKKAKELNIPVLSYPEAV
                     GEIMEEKRGIVVSGTHGKTTTSALVVNLLKTLNLSPSFLIGGEIINVGNSGVGDGEFL
                     VVEGCEYKRSFLNYHPEIAIITNIEKDHLDYYKDINDIKSAFFQFSQNIKSDGVLIYC
                     NEDRNVKSIVKKLKIKKLSYGFNSGNITCEGVRIRGRKTEFECFFNGKKLGKVNINLW
                     GKHNVLNSLAAVGVGIYLGFLWKEIKKGLENFKGVHRRCEILGEKSDIIVIDDYGHHP
                     TEIKSTLKCIKQVFPDKRLIVVFQPHQYSRTRFLLKDFAKSFSLADKIVVPDIYFVRD
                     SLIEKKLVNAEILVEKIRKNGKKAIYLPTFREIVEYLYEIKKRGDIILTIGAGPVDKV
                     AKEFLKRIK"
     gene            complement(9637..10695)
                     /locus_tag="PLF90_03250"
     CDS             complement(9637..10695)
                     /locus_tag="PLF90_03250"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOK56335.1"
                     /translation="MRKAIIITGPTGGHFFPGLAIGERLKDKFEIKFFVPKREYIVKH
                     LEKRNFTFRFIPTARITKKNFIFSTFKIFYLIIFSIILFLKEKPRIIIGTGSYVCVPF
                     IIGGRILCKKIIIHEQNYIPGKTTKILSPFVNFILLTFPYKNKLPSKKCIITGFPLIS
                     EYRENYRKEKIREIYGLENIKTILILGGSQGASFINRLIINNLSYFKKKGFQLVHIAG
                     KEKDFVEKKFFENNIKGKVYDFFYEMNKLYSAVDYVICRAGAGTLVEIVEKEIPAFLI
                     PYPYAGGHQVRNAEYLSKVGCAFFAEEKYIETGNFVDLFEKFLQESYKLKENLKKIKL
                     SDNGKIEEIIFTLFKEIT"
     gene            10740..11717
                     /locus_tag="PLF90_03255"
     CDS             10740..11717
                     /locus_tag="PLF90_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HOK56336.1"
                     /translation="MGKKAIGLISGGLDSVLALQMIKEQGFEIIGIFINTPFISKFGE
                     QIIKNLKKLSDEMNFKLLIIEAQEDYIEIVKNPEFGYGKNLNPCIDCHIYMLKKAKEI
                     MEKEKGEFVFTGEVLEQRGKSQNLNALKIIEEKSSLKGNLLRPLTALNLPPTEVEKRG
                     IVQRELLLGIKGRERKLQLYLAQIKNLKYFGTPSGGCLLTDAGFCRKLKDLFEHSSDI
                     NLNDCYILQIGRHFRISPKTKLIITRDERETAKIMNFCNENDLIIFSEKNPDVIGMIK
                     GEIENLIFEIYSSYLKKEDICFVKNLKGDILEKKIIEKKNKVDYRKFLL"
     gene            11714..12454
                     /locus_tag="PLF90_03260"
     CDS             11714..12454
                     /locus_tag="PLF90_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOK56337.1"
                     /translation="MNKKINLKERFYRSIVKRENFLKFEVKVEETDLLVMAKKNLKNE
                     IFNEVKKQREILKDYIRENPDFLFSFSPVYAKSDAEIIKLMSESSFLTKTGPMASVAG
                     AISEIIGKKFISESDEIIIENGGDIFAKMNSDFIVGIYAGNSPFSMKIGIKLKKREIP
                     YGIATSSGKLGHSISFGKADAVTVISPSSTFSDGAATYFGNLIKDKIDKQLIISELKN
                     FVFIEGIVIIKEKEIFLWGDIEIVNLTG"
     gene            complement(12465..13769)
                     /locus_tag="PLF90_03265"
     CDS             complement(12465..13769)
                     /locus_tag="PLF90_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOK56338.1"
                     /translation="MDTKALISLIIFIGTYFFIAIEKTPKVYVAFAGVILLVLFRVFN
                     FDEIHNYIDWDTITFLFGIFLVVKVIEISGFFNYFSLKIIKKFDYDPLKIFLFFPLLS
                     WFLSGFVDSITVLTFLTPLTYTLCRLLKTEPVSLIIAEVCLANIGGSGTLIGDPPNVI
                     LGSMFGFGFTNFFIHNYIISFLSGIGAISIFYRINREKLIKSRKNIDKKILEEIVPEE
                     AIEDKPLAKYGMLGLAGVVFLLIFRDFLKEIFPLSIGICSILPSLSILSLKGSHTKLK
                     NLLKEIDIETLLFFVSLFAIIGSLEKTEIIKNFALSLKKYSQNGIKMNTLLFWFSAFS
                     SAFIDNVPEAISIGYLIKHISNFIPYRFTILIWASSLGLDMGGNFTPIGASANVVAYG
                     FLESHGIRVGWKKWIKLMFLPNFVAVSISYILVILKFIAGFY"
     gene            complement(13769..14221)
                     /locus_tag="PLF90_03270"
     CDS             complement(13769..14221)
                     /locus_tag="PLF90_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOK56339.1"
                     /translation="MKKVKDYMDKDIPVVDKKTNLSEILKIFSNSLYNIIPVIDEDNV
                     LCGIICIEDILENLILSKKDVELLEKFPFFADAFSEITSTVECISSLLVAEDVMNKDV
                     IKIDENSSVMKALILMRKHHVNRLVVVNEKDNPVGFITRNNILKAIGC"
     gene            14415..15167
                     /gene="rpsB"
                     /locus_tag="PLF90_03275"
     CDS             14415..15167
                     /gene="rpsB"
                     /locus_tag="PLF90_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOK56340.1"
                     /translation="MEITIKELLEAGVYFGHPTRQFDPRIKPYLYGKRQGIYIIDIEK
                     TIEKIKESGEFLKKIASQNKKILFVGTKKQAQTVIKEIEENCKMPYVNYRWVGGTLTN
                     FNEIRKRIERLEEIEKLESSGKINLYTKKEIQLLEKEKEDLKKKFEGIREMTEYPGAM
                     VVVDVKREVNAIKEGKKLNIPIVGIVDTNGNPESVDYPIPANDDGLKSIQTVLLKLAD
                     YILEGLKESGYIQGNIKEEIKNEINSEEKEKK"
     gene            15164..15751
                     /gene="tsf"
                     /locus_tag="PLF90_03280"
     CDS             15164..15751
                     /gene="tsf"
                     /locus_tag="PLF90_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOK56341.1"
                     /translation="MKLDTSKIKDLREKTGLSVMECKKALEEANGNFEKALEILKKRG
                     LEISEKKAGKEAKEGLIGSYVHTNGKIGVLIEVNCESDFVAKNSEFEELVKNLCLQIA
                     AMKPKWIDVDSIPEEILKKKIEEIKEEYKDKPPSVLDKIVDGKLQDFYRENVLLKQQF
                     IKDEEITIEDYIKSKIAKLGENIKVKKFVRFEIGE"
     gene            15777..16064
                     /locus_tag="PLF90_03285"
     CDS             15777..16064
                     /locus_tag="PLF90_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56342.1"
                     /translation="MEFEVGYMMALVVIGISFLGFFLSMMVDEVNRKKGIIIFILSLI
                     LFGLGVYYYYVVSLWQKKSGGDSLNKLNYYLYLYRPAENTIPETQIPENIK"
     gene            16061..17911
                     /locus_tag="PLF90_03290"
     CDS             16061..17911
                     /locus_tag="PLF90_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing transglutaminase
                     family protein"
                     /protein_id="HOK56343.1"
                     /translation="MSCKYRIILFIFLFFSFFIHANNSENIYLIDFASVKLSSNYTKE
                     TLIHQKIKIENEKGKKFSHLQIPFDSEREKVKFIEGYTILPSGKKIKITSQDIKIVIP
                     AEFTEYSSLYPGYKVMSINFPGVEIGSIIEYKYQIITFKPLIEKHFWDGFYFQSTENF
                     ALSRYELKIPKKIKIQIYEKDIKLFEKKESFDYITYIWEKRNVPPIIEEILMPSLSEV
                     VPKVYVSTFSSWEEIGKWFFEISKTEKSKGKEIEEVVEQLIKNKKTEEEKIVSIYNYV
                     CSNIRYVGLEMGIHGYKPHHPEEVLKAKYGDCKDKANLLKKMLEIAGIKSYIALVNTE
                     RKVEKEIPFPGQFNHAILAVLKENNYLFLDPTTEVMKYPDIPPYEQDKYSLVCGENPE
                     LVKIPVSQPERNLRKRIVYASLDRSGNLTGEVTVIPSGIFEAGLRNAFRYLKQIERER
                     SLSKELNSILPGTKLISLEISGLESMENQLIEKYKFSTNNYGIKVGNKIIFQPALIDK
                     LVDLSIVSLEERKYPLRLNTYYRKEEIIEYKLPEKTEIEAIPSSVEINEEFAKFFNEF
                     KKTDSGLIFRRIFEINKMEISPEEYSRFKNFYRKVSFYDKLPVILNIKEE"
     gene            complement(17914..18183)
                     /locus_tag="PLF90_03295"
     CDS             complement(17914..18183)
                     /locus_tag="PLF90_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOK56344.1"
                     /translation="MIKKEIVEKISESTGLNKTIVKSVIDEFLNIMKNCLENTERIEL
                     RNFGVFYFKKVKEKKARNPKTGEEVIIPERTKIVFKVSKNIKKKK"
BASE COUNT         6781 a         2626 c         2308 g         6545 t
ORIGIN      
        1 atgtgaggca tcgtatataa attttactta gattcttata tgaggcaaca ggattttgaa
       61 aataaaaatt ttgagaagta taattttaaa aatatggaga aaataaaaca aaaaaaagaa
      121 gaaactctga aaaaaatctt aataagtata ggagttttat ttcttttttt tattttcaat
      181 ctctggcagt caggttcttt ttctcaatta aatcggatag ttaaaaatcc aaaattacag
      241 gtatttacaa ttgtctcact tgctatttta attgagggat ttccatttat tttaatcggt
      301 tctttaattt cgggtacaat tgaagttttg gtttcagaag ataaattcaa aaaaatttta
      361 cctcagggta agttttcatc tgcagtattt ggctcatttt taggtttttt atttcctgtc
      421 tgttcatgtg gagaaattcc tgtttcaaga agattgataa aaaagggaat accaactcca
      481 ggaggtattt cctatcttct tgcgactgca ataatcaatc caatcactct tttttcaacc
      541 tatcttgcct tttcctcttt aaaaatggtt tttggcagat tccttattgc ctttattgta
      601 agtgtttttg ttggtatttt aattgctcca gaagataaaa gtgaaatttt aaaggaatat
      661 gaagaaggac acaaacatga tgaaaacctt tcaaaatttg aaagaatttt tattcattca
      721 ggaaatgatt ttttgattat gggaaaatac ttaataatag gagcgacact tgcttctctt
      781 ttttcaacat ttacttcaag agaagtattt attgctatag gcaaaaataa agttttatca
      841 attctatcaa tgcaaattct ttctataatt ctttcattat gttcttatgc ggatgctttt
      901 gtagcgagtt catttactta cttacccgat atctcaaaaa ttatattcat gatttctgga
      961 ccaatgatga gtatatctct aatacttgta tattttgctg cattcaaaga aaaatttgtc
     1021 aaaaaacttc ttgtaagtgt attcttaacc ctttttattc ttggaattat tggaagtttt
     1081 ttaggaggat gagatatgaa aatttttaag aacagacagt tttatgaatt tttaattata
     1141 attttctata ttttgttttt tatctcatta atttttacag gaaatttcaa aatatttgtc
     1201 aaaaactttt attttccata catagtaata ggtgttttga ttttggtatt tctcctaaca
     1261 attgaaatta aaaaaatgaa aaattttaaa cagttaaatg tctatgatat tttttccttt
     1321 attatattcc tattcccttt aattttattt attcttgtca gaccaacggc tttaccaacc
     1381 tatgctgcat taaaaagagg aatacagaca gagtttctaa gcaaggatat tttaaaatca
     1441 ttacaggaaa agatagaaac agaagggaaa tacaaaaaac ttacaatcaa acaacttctt
     1501 gccctttcaa aatcaaaacc agaagaaata gaagaaaaag atgtgagcat agaaggtatg
     1561 gtatataaag gagaagaaaa agaaaagttt actttaataa gatttttaat aacatgctgt
     1621 gctgctgatg ccacaccact tggggttgaa gtagaatata aggggataaa agaaaaatca
     1681 acaggcgagt ggataccacc agaagaaaaa gaagaattta aaaatgagga ctgggtgaat
     1741 gtaaaaggaa aagttaaaat agaagaggga aaagtcaaaa taattgcaga agaagttaaa
     1801 aaaacaaatc caccatcaga cccttatctc tattaattaa aacaaattta acttgcaaaa
     1861 tttttaaatt tatgttataa ttaaatatta ccttccttca cccccctttt tcctctcctg
     1921 tcttccccgc aggagaggtt tttatttata ggttttctat cttgacaatt taaaagtata
     1981 accgtataat ataaatgaaa atcattttca tttagaaatg gaaaaatata aaaaatcgct
     2041 taaaaaaaat aagttaaaaa ttactcctaa aagggttgca ataataaact attttctcaa
     2101 aaatgataaa tattttactc catttgatat ctggaaatat atgaaaaaca actttaaaaa
     2161 aataggactt ccaacagtat atagaaacct tgcatatttt gaaaaaattg ggatattaac
     2221 aaaaatagaa ggtgaagaaa acagatttta ttatgggatt tgcaaagaag aaaagaatat
     2281 tcatcatcac catatagttt gtatctcatg tcataaagta gatgattttg gaatttgtaa
     2341 ttttgaagaa ataaaaaatg atatagaaaa gaaaaccggc tttattatta cagaacacca
     2401 attttttctt aaaggattat gtaaaaattg taagaggagg taaaatatga aaaaaataaa
     2461 gtttattgga gatgctcttt ctcatcatat acctttttct gttctctcag ttacaattgg
     2521 acttatattt gctggaattt taaccttttt tgttgaaatt ttaaatatag agaacccctc
     2581 tgaatatttt gaagaacttt tccatatttt tcatccaaca catatgcttt tctctgctct
     2641 tgcaacaact acaatgttct ggaaaaatga aaagaaattc tggaaggcat ttataattgg
     2701 agtaattggt tctgctggaa tttgtggaat aagtgatatt tttattcctt atatttctgg
     2761 atttctactc ggtgcaaaaa tgaaactcca tatatgtata attcaacatc ctcaaattgt
     2821 attgccattt ttatttctgg gggtttttat gggttatgtt gcacctgaaa cacttgaaaa
     2881 atcagaagga gttattttct ctcattcttt acatgtattt gtaagtgcaa ctgcctccat
     2941 attctatctt atcagtttcg gactaattga ctgggttcat cggataggga gtgtcttaat
     3001 ttatatggta cttgctgtag taattccttg ctgtacaagt gatatagttt ttcctcttct
     3061 ttttatttca aaaaaagata aattatctaa atacagtcac attcattaaa aaaaagtatc
     3121 tttcattatt tttacttcca gattatagaa aaagaaaaga tgtttttacc ttcagcagaa
     3181 acaataaatt ttccatcagg taaggattca agaaaattta atctatcttt taaaatatat
     3241 ctccataaac tttttttatc gtatgaaagg aagaataaaa aattcccttt ttttccagaa
     3301 atcaattcaa gaatttcaga agaaaaaata ttataattaa tttcttcatc atttctgaaa
     3361 ttttttattt catttccatt taaatcatat aaataaatca ttcctgtttc ggtttcaaca
     3421 caaatataat tttctccggg tttaatattt attattttac catttatatt tttttcccat
     3481 tttaggtttc catcatccct ataacagtat aacttcccat tttctgttcc tgtaaaaacc
     3541 agacttccat cgtcttttat attaacaaaa gaaataattt ctggtaagtt taaatcccag
     3601 aaaatctctc catttttatc aatataaagt attctcttat caattccagc aactataaat
     3661 ttaccatttt ctgatatacc tatccctctt cctaaattcc ctgtcttttt tgtccataaa
     3721 atctttccat ttcctgtaag aaaatataaa atcccttcaa gtgtaaagaa ggaaaaataa
     3781 tttccttcag gtgagattga aacttttata attttttctg acatatttaa ttttcgtatt
     3841 aaaactccct tatcatttat caaataagca cttccagaat cacatcctat taaaattctt
     3901 tttttatctc tggatgtctt aaggtcattt attaaatcat cggtttcata actccacaaa
     3961 aattttgttt ctggtaaaaa taaagaagat ggaatatcca gtccctgaaa gtttacagta
     4021 aatcttttaa tcatattagg aatatatctc atatcatctg ttttatactc ataatgctca
     4081 aaccactttt ttttgttaat tgaaaaaaca tccaaattaa aaacataagt attaacaatt
     4141 ttagcaagag atggcttaaa tacataatca gcattcagaa ttttacctgc aattgaaagt
     4201 tcttcatctt ctattgacat ttcaataaat ttctgctcat tcaaaacttt taaaaaactc
     4261 tctcttggaa caataataac attttttata ttattaattt catctgttaa tatacttgaa
     4321 aaatctgtct ggataaacgg aggaatgtca ataaatacag gattcagaat aataaccaaa
     4381 tttttcttaa acattaaatt taaatttttt atcttctttt ctatgtcact tatagaataa
     4441 tctttccccc agtaattttt attttcaata aattttgaaa tctcataata taaattaatt
     4501 gcttgattat acattccttc tttttcatag atactggcaa gcaacatata atagtccgca
     4561 tcaggaatta aacacttact cagataaact tcaaaatacc ttatactttc ttttatataa
     4621 ttagtatcta aacaaaaatc tcctattatt ctatagataa aagcaaccgg tgttgcatat
     4681 tttaaatatt ttaaactttc tttatctctt ggccatccaa atggccagtt attaggagaa
     4741 aacggctcac atttttctgt tttttcttta gcagttctta aatatgcatc tgcagatttt
     4801 atatttcctc tatttgaata ataatttgct ttttcaacta acttccagac ctcatttgaa
     4861 aaaacaactg aaagttgttt gttcttttca taccagtttg ccccaaatcc attagcaaat
     4921 aaaataaaaa gagtaataaa aataataaat tttctcatgg gctctccttg aaaataatta
     4981 aaccaaactg gtctggaata tctattctac ctgtccatgt tgatataata taattattta
     5041 tctctctctg aaagtttatt gtccattgag ttcctttgat aattttttct acttttaaat
     5101 ctttaatagg tatggctatt tcagcaatcc attttctaac tgttccaggg gaagaaacat
     5161 ctgtatcaat tttccaatca gaattccata atttatccac atcttcttca tttttataaa
     5221 atttccattt gtaactgtat ttaagatatc ttacttcttc agaaagtgga aatgtaaact
     5281 taaggggatg aaaaacgaat tgataccatt cattaaaaga attctttgga tttatagaaa
     5341 ttgttaaaaa atctcttgta tcaaaattta ttacaccatt tgatggattt tcacatttaa
     5401 ttaaaaagta aatattttca ttgtcataaa cactcgctat ttttgtttga gcatcaataa
     5461 aaactttttc atcttctata cttataaatc cattaatttc ctcaatcatt ttccatattc
     5521 cttcatcatc ttttccatct atttcaactg gaatatctgt tttatatgaa tacattatct
     5581 tcggacttac ctcctgaact cctaatttat ttctatatat ttcctctcta ataaaaggta
     5641 tcttacttct gagattagaa ggaatttttt ggtcctttag ataaatattt gcaagtgttc
     5701 ttataggttc aacatttatt tcagacaatc ttacaagaac ttctaaacct gttaaatttc
     5761 ctaaccttgc gagtccccat gcagaaataa tttttatatc aagttctgag tcatgaagtt
     5821 ttttaataaa tataggtgaa tacgctatct tatctaaata aataagagat tcaactgcaa
     5881 ttttctgata ataaacatca tcattattaa tatttttcat aatcacatta atcccgtcgt
     5941 ttctacccat ttttgcaaga gaattggcac atataagttg atattcaggg tcaaaatgag
     6001 ttaatccctg ttttaaaatt ccaattgaac ttaaatctcg catttctcct aaaacaatag
     6061 cacataattt ttttaattct acatctgatt cttttgcaag ttctttaaca ttttttgcaa
     6121 atacttttct gggcatctct tttaaaacaa aaaccgcttt ttctctaact tctggatatt
     6181 tactataaaa catcttaatt aacaacttat ttatttcatt acttcctatg aaactatcta
     6241 ttttatcaat aacaaatttt ctatcgcttt ctctcatact atttatttta ataatgcatt
     6301 catctattat ttcacctaaa tatcttgaac tgcactttct aattcttata tcagggtcat
     6361 tcttttccat ataaacaaga tttttaatgt aataatcatc aaatttatca agataataga
     6421 aaccttcaat agaagtatat cttatttctc tatcagaact ttttaaatat tttttgagta
     6481 tatcttttgc tctgtttaaa tcaagatttc ctatttttcc aagttcaatt atagccagtt
     6541 ttttatgctc tatgtttgtt tctttattct caataaactt ttctaaaata ggaataaata
     6601 tagaatcatc agttctttca ataatattta ttgcttcttc tctaacactt agggacttat
     6661 ctgcaataca tttaactatt tcaggataag aagcaggatt tttaaatttt tcaagtgccc
     6721 ataaagaata tatctttaca ctctcatttt catcactcaa tcctttaatt aaaccattta
     6781 ttccttcttc tccttttaat ctaacaagag ttttaatcgc ttcaatcttt acctttacag
     6841 atggttcatt caataaaccc tcaatttctt ctatattttt cgttttaaat ccttcaagtg
     6901 cccataatga tttaatcttc acctcatcac ttttatctcc cagcaatttt acaagatatg
     6961 gaatactcct tacatctttc acactttcaa aaatagaaat tgttaataat tttatctgct
     7021 catcattact ttcaaaacaa tcaaaaagta attttaattg attttcatcc attgacttaa
     7081 aattaaatct tgttatgttt ttccataata atctctttaa tgatatcaga attgcttttt
     7141 ttacttctaa atttgtctca ttaagatgtg aaataagatt agagattgaa ttttcgtctc
     7201 catattttcc aatacctatt gcagcatagt atcttacctg ccagtatggg tcatcaagtg
     7261 aagcaaataa attctcaaga atatcccctt ctccaaattt aacaagtcct tttattccat
     7321 ataatcttat ctgccagtac tcatcaccaa gagatttttt gtagaaatca atacaggtag
     7381 aatctcctat ttcataaagt attaaagatg caaaagttct aacttcttta tcttcatcat
     7441 ctaaaaaatt agcaacttct tttatataaa ccttatcttt attgtttaaa acttttttca
     7501 gaaattcaat cttctttgtc tttgtcccga atgtaagaac ctgagattca ccatatatac
     7561 taaaatttag taaatataaa aaaataaaaa ttttacttat ctttctcata atagtatttt
     7621 ggaaaaccaa tatcgactaa tttaattttt cctacatatt caggtccttt accagtaaag
     7681 aaaccttttt ttaaaaatcc catgcttatt gttatattac ttttaacaga aacaccgtgt
     7741 atttcacctg tgtcagcatc aagccctgat ggtatatcaa ttgacacagt ataaattgaa
     7801 cttttattta taaactctat tacatttctc atatcacctt ttatttcacc ttttattcct
     7861 atccctaaaa tagcatctat aaaaacgtcc tttctttcaa ttttcaaccc ttttaatttt
     7921 aaatcattta tactctctgt gtcaattttt aatcttctca atatttcaaa atttaaaaaa
     7981 gaaatatcgg taaaatcttc tggtttgcca aggtaaaaaa ctttaacatt tgctccatta
     8041 ttaaataaat atcttgtaag aacaaaacca tcacctccat tattaccctt accacacaaa
     8101 ataaaaaact ttctattttt tatatctttc aatatttttt ttattacata aaaactttct
     8161 cttcctgcat tttccataag aatcaaattg ctgataccaa atttttcaac tgtttctttt
     8221 tctattttta atatctcttc ccttgttaaa attatttttt tcatttaatt ctttttaaaa
     8281 attcttttgc aactttatct acaggacctg cacctatagt caaaattata tctcctctct
     8341 tttttatttc ataaagatat tctactattt ctctaaaagt tggaagataa attgcttttt
     8401 taccattttt tcttattttc tcaacaagaa tctctgcatt aacaagtttt ttttcaatta
     8461 atgaatccct gacaaaatat atatcaggaa caacaatttt atctgcaagt gaaaatgatt
     8521 ttgcaaagtc cttcaataaa aatcttgttc tgctatattg atgtggttga aatacgacta
     8581 tcaacctttt atcaggaaat acctgtttta tacatttcag tgttgatttt atctcagttg
     8641 gatgatgtcc ataatcatca ataacaataa tatccgattt ttctccaaga atttcgcatc
     8701 tcctgtgaac tcccttgaaa ttttcaagtc ctttttttat ttctttccat aaaaaaccaa
     8761 ggtaaatccc aactcccaca gcagcaagag aattcaaaac attatgttta ccccatagat
     8821 ttatattaac ttttccaagt tttttcccat taaaaaaaca ctcaaactct gtttttcttc
     8881 ctcttattct aactccttca catgttatat ttcctgaatt aaacccgtaa gataattttt
     8941 taatttttag ttttttaaca atacttttaa catttctatc ttcattacag taaattaaaa
     9001 caccatctga tttaatattt tgagaaaact gaaaaaaagc actttttata tcatttatgt
     9061 ctttatagta atcaagatgg tctttttcaa tatttgtaat gatagcaatt tctggatggt
     9121 aatttaaaaa tgatctttta tactcacaac cttctacaac aagaaactca ccatcaccca
     9181 cacccgaatt tcctacattt ataatttcac cccctattaa aaaagatggt gataaattta
     9241 atgtctttag aagatttacc acaagggcag aagttgttgt tttcccatga gtacctgaaa
     9301 caacaattcc ccttttttct tccattattt ctccaactgc ttccgggtaa cttaaaacag
     9361 ggatatttaa ttcttttgct tttttatatt caggattatc aggtaaaatt gcctgagaat
     9421 aaataacaag gtctatatct gaatctatat ttttttcatt ctgtccaatg tacaccttta
     9481 ttcctaattt tctgagtttt ttaataattg gtgaataatt attatctgaa ccggaaactt
     9541 ttttatttaa acgaagtaaa attttagcaa gaccacttac accaatgcca ccaatcccga
     9601 tgagatgtat tctttttgag ttttttataa atcctttcat gttatctcct taaaaagtgt
     9661 aaaaataatc tcttcaattt taccattatc agaaagttta atttttttca aattttcctt
     9721 taatttataa ctttcctgta aaaacttttc aaataaatca acaaaattcc cagtttcaat
     9781 atatttttct tcagcaaaaa aagcacaacc aacttttgat aaatactcag catttctgac
     9841 ctgatgacct cctgcatatg gataagggat taaaaaagca ggaatttcct tttcaactat
     9901 ttctacaaga gttcccgctc ctgctctaca tataacataa tcaactgcag aatatagttt
     9961 attcatctcg taaaaaaaat cataaacttt tccctttata ttattctcaa aaaatttttt
    10021 ttctacaaaa tctttctctt ttcctgctat gtgtacaagt tgaaatcctt tttttttaaa
    10081 gtaactgaga ttatttatga ttaatctatt tatgaaagaa gcaccctgac ttcctccaag
    10141 aattaaaatt gtttttatat tttcaagtcc gtaaatttct cttatctttt cttttctata
    10201 attttctcta tattctgaaa ttaatggaaa tcctgttata atacattttt tagaaggtaa
    10261 tttattttta taaggaaatg ttagtaatat aaagtttacg aaaggagata aaatttttgt
    10321 ggtctttccc ggtatgtaat tttgctcatg aattattatt tttttacata aaattctacc
    10381 cccaataatg aaaggtacac atacataact tccagttcct attattattc gtggtttttc
    10441 ttttaaaaag agaataattg aaaaaattat aaggtaaaaa attttaaaag tggaaaaaat
    10501 aaaatttttt tttgtaattc ttgctgttgg aataaaacga aatgtgaaat tccttttttc
    10561 aagatgttta actatatatt cccttttcgg aacaaaaaat ttaatttcaa acttatcctt
    10621 taatctctct cctattgcaa gaccaggaaa aaaatgtcca ccggtaggtc ctgttattat
    10681 aattgctttt ctcatagatt taattatatc atatataaag aataacattc actaaaaata
    10741 tgggaaaaaa agcgataggt ttaatatcag gaggtcttga tagtgttctt gcacttcaga
    10801 tgataaaaga acagggtttt gagattatag gtatttttat aaatacaccc tttatttcaa
    10861 aatttggaga acagataata aaaaacctga aaaaattgtc cgatgaaatg aattttaaac
    10921 ttttaattat tgaagcacag gaggactaca ttgaaattgt aaaaaaccct gaatttggat
    10981 atggcaaaaa tttaaatcca tgcattgatt gtcatatata catgttaaaa aaagcaaaag
    11041 aaattatgga aaaagaaaaa ggagagtttg tatttacagg agaagtcctt gaacagaggg
    11101 gtaaatcaca aaatttaaat gctttaaaaa tcattgaaga aaaatcatca ttaaaaggga
    11161 atcttttaag acctcttaca gcattaaatt taccacctac agaagttgaa aaaagaggta
    11221 ttgtacaaag agaactttta cttggtatca aaggaagaga acgaaaattg caactttatc
    11281 ttgctcaaat aaaaaatttg aaatattttg gaacaccttc tggtggttgt ttactgacag
    11341 atgctggatt ttgtagaaaa ttaaaggatt tatttgaaca ttccagtgat ataaacttaa
    11401 atgattgtta tattctacag ataggaagac atttcaggat ttctccaaaa acaaaattga
    11461 ttattacgag ggatgaaaga gaaacagcaa aaataatgaa tttttgtaat gaaaatgatc
    11521 ttataatttt ttctgaaaaa aatccagatg taataggtat gataaaagga gagattgaaa
    11581 atttaatctt tgaaatttat agttcttatt tgaaaaaaga agatatatgt tttgttaaaa
    11641 atttaaaagg agatatactg gaaaaaaaaa tcatagagaa aaagaataaa gtagattaca
    11701 gaaaattctt attatgaaca aaaagataaa cttaaaggaa agattttata gaagtatagt
    11761 aaaaagagaa aattttttga aatttgaagt taaggtagag gaaaccgacc ttcttgttat
    11821 ggctaaaaaa aatctaaaaa atgaaatttt taatgaggtt aaaaaacaaa gagaaatttt
    11881 gaaagattat attagagaga accctgattt tttattctct ttttctcctg tttatgctaa
    11941 aagtgatgcg gaaattataa aacttatgtc agaaagttca tttttaacaa aaacaggacc
    12001 aatggcttca gtagcaggtg caatatctga aataatagga aaaaagttta tttctgaatc
    12061 tgatgaaata attattgaaa atggaggaga tatttttgct aaaatgaata gtgattttat
    12121 agttgggatc tatgctggaa actctccttt ttcaatgaaa ataggaataa aacttaaaaa
    12181 aagggaaatt ccatatggaa ttgcaacttc aagtggaaaa ctaggacact caataagttt
    12241 tggaaaagca gatgctgtta cagttatttc cccttcttca acattttcag atggagcagc
    12301 gacttatttc ggtaatttaa ttaaagataa aatagataaa caacttatta tttccgaatt
    12361 gaaaaatttt gtatttattg aaggaattgt tattataaaa gaaaaagaaa tttttttatg
    12421 gggagatata gaaattgtaa acttaactgg ttgaaattaa aaaattaata aaatcctgct
    12481 atgaatttta aaatcacaag aatataactg atacttacag caacaaaatt gggtaaaaac
    12541 attaatttaa tccacttttt ccaaccaact cttataccat gggactccaa aaatccataa
    12601 gcaacaacat ttgcacttgc tcctattgga gtaaaatttc cgcccatatc aagtcctaaa
    12661 cttgaagccc atattaaaat agtgaatcta taaggtataa aatttgatat atgttttata
    12721 agatagccaa tacttattgc ctcaggaaca ttatcaataa aagcacttga aaatgcagaa
    12781 aaccagaata aaagtgtatt cattttaatt ccattttgtg aatatttttt aagggacaaa
    12841 gcaaaatttt ttataatctc tgttttttca agagaaccaa taattgcaaa aagagaaaca
    12901 aaaaataaaa gtgtctcaat atctatttcc tttaataaat ttttcaattt tgtatgactc
    12961 ccttttaaag aaagaatgga gagagaggga agaatactac aaatccctat acttaatggg
    13021 aaaatttctt ttaaaaaatc tctaaaaata agcagaaaaa caactcctgc aagacccaac
    13081 attccatatt tcgcaagtgg cttatcttct attgcttctt cgggaactat ttcttctaaa
    13141 atctttttat ctatattttt tctactttta ataagttttt ctctgttaat cctataaaaa
    13201 atggaaattg cacctattcc agataaaaaa ctaattatat aattgtgtat aaaaaaattt
    13261 gtaaaaccga aaccaaacat tgaaccaaga ataacattag gtgggtctcc tattaaagtc
    13321 ccggaccctc caatattagc aagacaaact tctgcaatta ttaaagacac aggttctgtt
    13381 tttaacaatc tacaaagggt atatgtaagt ggtgtaagaa aagttaaaac tgttattgaa
    13441 tcaacaaatc ctgataaaaa ccatgaaagt aatggaaaaa acagaaaaat ttttaaaggg
    13501 tcatagtcaa atttttttat aatttttaag gagaaataat taaaaaatcc agatatttca
    13561 ataaccttaa ccactaaaaa aatcccgaat aaaaaagtaa tagtatccca atcaatataa
    13621 ttgtgaattt catcaaaatt aaaaactctg aataatacca gaagaataac accagcaaaa
    13681 gctacataaa cttttggtgt tttttcaatt gcaataaaaa aataggttcc aataaaaata
    13741 attaaactta taagtgcttt tgtatccatt agcatccaat tgcttttaaa atattatttc
    13801 ttgttataaa gccaacagga ttgtcttttt cattcacaac aacaagacga ttaacatgat
    13861 gttttctcat caatatcaac gctttcatca cagaagaatt ttcatctatt tttattacat
    13921 ctttgttcat aacatcttca gcaacaagta aagaactaat acattcaaca gtactggtta
    13981 tttctgaaaa agcatctgca aaaaaaggga acttttcaag taattcaaca tcttttttag
    14041 ataaaatcag attttcaagg atatcttcaa tacaaataat accacacagg acattatctt
    14101 catcaataac cggaataatg ttataaagag aatttgaaaa aatttttaaa atttcactta
    14161 gatttgtttt tttgtcaaca accgggatat ccttgtccat ataatcttta acttttttca
    14221 tttttttcct cttttatagt ataattaaat taagttttct tttatcattc atttttgatt
    14281 attagtatat aacataatag tcaatttgac aaaattttgt tggaaatgaa aaattataaa
    14341 aaaaagaaaa attttgttga caatttataa aaaaatttta taatattaac tcttatgaaa
    14401 tagaaaggag aaaaatggaa ataacaataa aagaattact tgaagcgggt gtatatttcg
    14461 gacatccaac gagacagttt gacccacgaa ttaaaccata tctttatgga aaaagacagg
    14521 gaatttatat aattgacata gaaaaaacaa ttgagaaaat taaagaaagt ggagaatttt
    14581 tgaaaaagat tgcttcgcag aataaaaaaa tcctttttgt cggaacaaaa aaacaggcac
    14641 agacagttat aaaggaaatt gaagaaaact gtaagatgcc ctatgtaaat tatagatggg
    14701 taggaggaac tttaacaaat ttcaatgaaa tcaggaaaag gatagagaga ttagaagaaa
    14761 tagaaaaact tgaaagttct ggtaaaataa acctttatac aaaaaaggaa atacaacttt
    14821 tagaaaaaga aaaagaagat ttaaaaaaga agtttgaagg gattagagaa atgacagaat
    14881 atcctggtgc aatggtagtt gtggatgtta aaagagaagt aaatgcaatc aaagaaggta
    14941 aaaaattaaa tattccaata gttggaatag ttgacacaaa tggaaatcct gaaagtgttg
    15001 attatcctat tcccgctaac gatgatggat tgaaatctat tcaaacagta cttcttaaac
    15061 ttgctgatta tattcttgaa ggactaaagg aatcaggata tattcaaggg aatataaaag
    15121 aagaaataaa aaatgaaata aattcagaag aaaaggagaa aaaatgaaat tagatacatc
    15181 aaaaattaag gatttaagag aaaaaactgg tttaagtgta atggaatgta aaaaggcact
    15241 tgaagaggca aatggtaatt ttgaaaaagc acttgaaata cttaaaaaaa gagggcttga
    15301 aatttcagaa aaaaaggcag gaaaggaagc aaaagaaggt ttaattggtt cttatgtaca
    15361 tacaaatgga aaaatcggtg ttttgataga agtaaattgc gaatccgact ttgttgcaaa
    15421 aaatagtgaa tttgaagaac ttgtgaaaaa tttatgtctt caaattgctg caatgaaacc
    15481 taaatggata gatgttgatt caatacctga ggaaatttta aaaaagaaaa tagaagaaat
    15541 aaaagaagag tataaggata aacctccttc tgttctggat aaaatagttg atgggaaatt
    15601 acaggatttt tacagagaaa atgttctttt aaaacaacag ttcataaagg acgaggaaat
    15661 aacaatagaa gattatataa aatcaaagat tgcaaaattg ggtgaaaata ttaaagtaaa
    15721 aaaatttgta agatttgaaa ttggagagta aaataaaaaa ataacgggag gtaaaaatgg
    15781 aatttgaagt tggatatatg atggcgcttg ttgtaattgg aatcagtttt ttaggttttt
    15841 ttctatctat gatggtagac gaagtaaata gaaaaaaagg aattataatc tttattctat
    15901 ccttaatcct tttcggatta ggagtttatt actactatgt agttagttta tggcagaaaa
    15961 aaagtggtgg agattcacta aataaactaa attattattt atatctgtat agaccggcag
    16021 aaaacacaat tccagaaacg caaatacccg aaaacattaa atgagttgta aatatagaat
    16081 aattttattt atttttcttt tctttagttt ttttattcat gccaacaata gtgaaaatat
    16141 ttatctgatt gattttgctt ctgttaaatt atcctcaaat tacactaaag aaacattgat
    16201 acaccaaaaa attaaaattg aaaatgaaaa agggaaaaaa ttcagtcact tacagattcc
    16261 ttttgattca gaaagagaga aagtaaaatt tattgaagga tacacaatat taccttcagg
    16321 aaaaaaaata aaaataacat ctcaggatat aaaaatagtt attcctgctg aatttacaga
    16381 gtattcatcc ctttatccag gatataaagt aatgagtata aattttcctg gtgttgagat
    16441 tggaagtatt attgaatata agtatcaaat tattacattt aaaccactta tagaaaagca
    16501 tttctgggat ggtttttatt ttcaatcaac agaaaatttt gcactttcaa gatacgaatt
    16561 aaaaattcct aagaaaataa aaattcaaat atatgaaaaa gatataaaac tatttgaaaa
    16621 aaaggaaagt tttgattata taacttatat ctgggaaaaa agaaatgttc caccaattat
    16681 tgaagaaatt ctgatgccat ctttaagtga agtagttcca aaagtttatg tttctacttt
    16741 ttcatcatgg gaagaaatag gaaaatggtt ttttgaaata tcaaaaacag aaaaaagtaa
    16801 agggaaagaa attgaagaag ttgttgagca acttataaaa aataaaaaaa cagaagaaga
    16861 aaaaattgtt tcaatttata actatgtatg ttcaaatatc agatatgttg gacttgaaat
    16921 gggaatacat ggatataaac cgcatcatcc agaagaagtt ctaaaagcaa aatatggtga
    16981 ttgcaaagat aaagcaaatt tattaaaaaa aatgcttgaa atagctggaa taaaatctta
    17041 tattgctctt gtaaatacag aaagaaaagt agaaaaagaa attccttttc ccggtcagtt
    17101 caatcatgca atacttgctg tattaaaaga gaacaattat ttatttcttg acccaactac
    17161 tgaagttatg aaatatcctg atatcccgcc ttatgaacag gataaatatt cacttgtatg
    17221 tggagaaaat cctgaactgg taaaaattcc agtttcacaa cctgaaagaa atttaagaaa
    17281 aagaatagtt tatgcaagtt tagatagaag tggaaattta acaggtgaag ttacagtaat
    17341 cccttctggt atttttgaag caggattaag aaatgctttc agatatctaa aacagataga
    17401 aagagaaagg tcattaagta aagaattaaa ttcaattctt ccaggaacaa aattaatttc
    17461 tcttgaaata tcaggtcttg aatcaatgga aaaccaattg atagaaaagt ataaattttc
    17521 aacaaataat tatggaataa aagtaggaaa taaaatcatt tttcaacctg ctcttataga
    17581 taaactggtt gatctttcta ttgtttctct tgaagaaagg aaatatccat taagattaaa
    17641 tacatattat agaaaagagg aaataataga gtataaactt ccagaaaaaa cagaaataga
    17701 agcaattcca tcatctgtag aaataaatga agaatttgct aaatttttta atgagtttaa
    17761 aaaaacagat tcaggtttaa tttttagaag aatttttgaa ataaataaaa tggaaataag
    17821 tccagaagaa tactcaagat ttaaaaattt ctatagaaaa gtatcattct acgataaatt
    17881 acctgtaatt ttaaatataa aagaagaata attttatttt ttctttttta tattttttga
    17941 taccttgaaa actatcttag ttctttcagg aataataacc tcttctcctg tttttggatt
    18001 tcttgccttt ttttccttta cttttttaaa gtaaaacact ccaaaatttc tgagttcaat
    18061 cctttccgtg ttttcaagac aatttttcat tatatttaaa aactcatcaa ttacactctt
    18121 aactattgtc ttatttaaac ccgttgattc tgatattttc tcaacaattt ctttctttat
    18181 catagataaa tccctttttt aagaatgccg gaggagggat ttgaaccctc acggagtttg
    18241 ggctccaccg gattttgagt
//
LOCUS       DAOSAT010000024        17911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2179_length_17911_cov_5.428595, whole genome shotgun sequence.
ACCESSION   DAOSAT010000024 DAOSAT010000000
VERSION     DAOSAT010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17911
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2179_length_17911_cov_5.428595"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   19..285
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19..56
                     /rpt_unit_seq="gtcgcaatcccctttaaagcaggtcagaaattctaacc"
     gene            complement(463..1725)
                     /locus_tag="PLF90_03300"
     CDS             complement(463..1725)
                     /locus_tag="PLF90_03300"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK56345.1"
                     /translation="MEKIKDILLLQKREIERKFEERFIERDIDKRKFENDLIKVIIGP
                     RRAGKSFFGIHFLKEKGDFGYINFEDERIINIKNYDDLINALKIVYRKPKILFFDEIQ
                     NIGNWEIFVNRLQREGFNIILTGSNSKLLGRDLSTHLTGRHISVFVFPFSFQEFLRIE
                     GKELTTSEIKSKLIQYINFGGFPEPHIKKIDFKDYLSMLFNSLIYKDIVKRYRIRKPD
                     VIENLAIYLISNTGKEFSYNTLSKITGARSVHTIERYIDYLEEGFLIFRVNKFSFKLK
                     QMITSNKKIYCIDNGFIYTCSLKATEDTGFLYENAVAVELKRYEMEGKIKLYYWKNEK
                     GEEIDFVVRKGTDTISLIQVAYEISDIKTKKREIRALLKGKEELRCENLIILNNEYKA
                     EEEIKWFGIKGRVKFIPFWEWFIERNKF"
     gene            complement(1826..2101)
                     /gene="cas2"
                     /locus_tag="PLF90_03305"
     CDS             complement(1826..2101)
                     /gene="cas2"
                     /locus_tag="PLF90_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002788615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOK56346.1"
                     /translation="MFVVISYDIQDDKKRFKVSQILENYGTRVQYSVFECIIDDEQLK
                     KIKEKTGEIIDKENDSIRFYIICEGCLKKVEIIGIGEITKDMDFYII"
     gene            complement(2103..3092)
                     /gene="cas1"
                     /locus_tag="PLF90_03310"
     CDS             complement(2103..3092)
                     /gene="cas1"
                     /locus_tag="PLF90_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOK56347.1"
                     /translation="MATLYITEQGAKLKKEDKRFVIEKEDSVIFEIPEFKVDKVFIFG
                     NIQITTQALKFLLTSGIDTSFFTIYGKFIGKLQPVESKNVLLRIKQFEKYNSDEFRVE
                     ISKKFVEGKINNIKNFLQKYQRNHPEIDFSGEIKEIEENLEELKRKNSVSSIVGLEGR
                     CSAIYFDCFGKMILKNFEFEKRVPRNPKDPVNSLLSFGYSILTSEVFSILQAIGFDPY
                     LGFMHSVEYGRPALALDLMEEFRQVIIDRLVLEIINKEIIKGDGFREEEEGKIYLNEA
                     SIKEYLKQYERRILTEINYEDQTMNYRKLIQHQIRKFEKTILENCEYLPFELK"
     gene            complement(3095..4375)
                     /locus_tag="PLF90_03315"
     CDS             complement(3095..4375)
                     /locus_tag="PLF90_03315"
                     /inference="COORDINATES: protein motif:HMM:NF021207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HOK56348.1"
                     /translation="MSRRILIITVGGSDEPVVSAIKGYNPDFIYFICSGGKPEVASSI
                     TIDGKDKNGRVINEKPCLRKKEIKCKKCGEIIEKGEKYPPIIEQAEYSESYEKVVLND
                     PDDFNEVYEKTKQTIEKALKEGDEIICDFTGGTKTMSSVLAILTAFEFKTKPSIVKGK
                     REDIIKIKELSFPVLENVNSARVDFLLQIVNEFISRYLYYPAKVILENILTYSTFDRE
                     TQKKIIEKCELCTCFYYWDIFEYDKSLSILKNYGGRFKDHLNYLLKICEKITGYESVF
                     DLVANAERQAHNSFYDNATARLYRALELFAQKRLKNKYGIETSSVDIEKVKNKEKWEI
                     KKDKEGKIKIGLVDSYELLDELGDEIGKIYREKEKKFKNILNIRNTSKLAHGDRPVNK
                     DDWKKILKFIKDFINDCCNQIKIKIEYFQLPNNF"
     gene            complement(4372..5283)
                     /gene="csm5"
                     /locus_tag="PLF90_03320"
     CDS             complement(4372..5283)
                     /gene="csm5"
                     /locus_tag="PLF90_03320"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HOK56349.1"
                     /translation="MKIKIKVLSPVHIGSGKEISPLEYVLEENKFIRINMDALFQSPE
                     FSKFKDKFLNMAQSERYIKNILPLDLLLSCTLYQIDISKTTEKFNPIEVKEFIKSCGS
                     VYIPGSSLKGSILSGLMEEVLYKKNIKKFTNFENHLAEVLSEITGKYDRGKFAQYLIV
                     RDSNFKKPEESLELSLSKLIGAKTQNKLPILYETLKINTEFETEIKTTDDCKFKEEEI
                     LSMADRFYREVYKKEKEYATGKIIILPEPPKDGYLLRLGQGSTAWATSFLILSEKLKI
                     FYKVQKPKTRKLISGAISMGWVSIQII"
     gene            complement(5273..6223)
                     /gene="csm4"
                     /locus_tag="PLF90_03325"
     CDS             complement(5273..6223)
                     /gene="csm4"
                     /locus_tag="PLF90_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm4"
                     /protein_id="HOK56350.1"
                     /translation="MKIVKIKFKSSFHSGEREGFLEATEEIIHSDTLFSAFCYNYLLL
                     YGENELNQLIEKFRNNNPVFLISSMFPYSKEKLYFPVPLNQLPKEKVIKKIKYIEKDG
                     FEKLLNGEKLEDIYLNYSTIPEKDGKTHYNIIENPRIGLSRLNTTPGENYFHFGEVFF
                     EEGSGLFFVVDFKEPDFEKKFFATLRLMGDEGIGGDRSVGKGNFTIDTIDKFEIQTPS
                     ESFGCIILSLYLPQENELKDLKDGYYEIIERKGYIFSPYTKSLRKKSIRMFIEGSVFP
                     SMKKGKIADVTPTEIFQSHNVYRYGFAFSLPCKIEVQNEN"
     gene            complement(6236..7063)
                     /gene="csm3"
                     /locus_tag="PLF90_03330"
     CDS             complement(6236..7063)
                     /gene="csm3"
                     /locus_tag="PLF90_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HOK56351.1"
                     /translation="MLNENKIKVNLLGKILIKGDIKVITGLRIAGATTGLKIGGVDQP
                     VITDAFGRPYIPGSSLKGKLRMLAEKNEKVKLNKTRNVKNSKKEEPYWHECDAPEEYL
                     KCKICKVWGILSSDKLLGIPVGTRLIVRDIFLDEKSITEEMRRNMELEWTEIKMETAI
                     DRLKGTALEKSLRTIDRVPAGAVFRPMEMVFNIFEESDKNLLKVVFESMELLEDDYLG
                     GMGSRGYGKIKFENIEIYWNKKEDYESGNINVYKINDGYATPKDIVKNFSNIQNNIT"
     gene            complement(7076..7471)
                     /gene="csm2"
                     /locus_tag="PLF90_03335"
     CDS             complement(7076..7471)
                     /gene="csm2"
                     /locus_tag="PLF90_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HOK56352.1"
                     /translation="MTNINKEIIEKIINGDFEILNKYAQEIGEKYAPQNPREKEEKLT
                     TSQIRNILDDVQRMKEEDIKSQKHQILRPKLAYIAGRNNKSFALKELKEIIDYALQLV
                     GDDYKKFENFRYFFEAIVGYHRFYSKVKD"
     gene            complement(7737..10130)
                     /gene="cas10"
                     /locus_tag="PLF90_03340"
     CDS             complement(7737..10130)
                     /gene="cas10"
                     /locus_tag="PLF90_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HOK56353.1"
                     /translation="MNERESLILGSLLHDIGKFSHRAEEKLRLEYDKNLRTFYCPTDY
                     GYSHLLYSGQFVKDIFKDKESPIENLVLSHHKPDSFKGDKKLPKIIQLADYLSSGERR
                     ERDESEKGVVKKEPIISIFSQLLIKDEKNGEIYKPEELYNRPVKIDNNLEKLVPVEKK
                     DAITDNYNFKKLWEEFKDECQYLNYPSNFEKIFTQIFSLLEKYTLFIPSSAYTDKPEI
                     SLFHHLKLTCAIATCLYDLNIAKETLDKLIEELKKIFEKRRTGELIEEIDYGKLNRKD
                     FILLKGDISGIQDFIYSVSSEHALKGLRGRSFYLQLISEIIAYKILDEFNLTKANLIY
                     LGGGNFIILLPNLSDAEKRLEKLTEEIDKVIFKAHQGKLGFLISYIKFPYWNFIENFG
                     DLLSELGRKLAIEKRKKFKYLISENLFKPYPEKVEKEMGGCEICGREIEKKDKCELCE
                     SFEKLAIEIKKENVIMEIKKVNKKEIKEEVNKWDEIFEGLGYKISFEKSIGGIKIYLN
                     NSDFLKVNCDAFRYEAIYSPEGTLEDIAKKAEGIERWGALRMDVDNLGLIFGSGFIDE
                     EGKDITTISKYSMLSYTFSLFFSLGIRNIVEKEYKEKCCVVYSGGDDLFIIAPWSDLP
                     EIAEEIWRNFRKYVCNHEKITISGGIYIAPSKKFPVYQAAKKAGEEEEKAKKGEKNKI
                     TFFEPIKWDEEFVKIKEVKNKLYELLKEDGKPKVSRAILTILYGGYKEKELKERGKIP
                     FVRIWRIFYALKRFMERHKDYENDIDKIRETFITDFNIQPKLNVAVRWAELLTRKGG"
     gene            10231..11145
                     /locus_tag="PLF90_03345"
     CDS             10231..11145
                     /locus_tag="PLF90_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637703.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOK56354.1"
                     /translation="MELYKKFSDYLRKRFGCRVHKISVYAGFSCPNIDGRLSDKGCIY
                     CNNYAFSPSLREILPLEEQIKKGIEYGKRRFKAEKFIVYFQPYSNTYAPLEILKEKYD
                     VVKKFPEVVGISIGTRPDCIDEEKLDLIESYSSNYEVWIEYGLQSIHDKTLKIINRNH
                     TYSDFLKVYRMTRKRNVKICAHVIIGLPYETKEEIIETGKECGILELDGIKIHPIHIL
                     KNTELEKWYKEGKFKVMDMEEYIDLLIDFLSYLYPETVIQRLTADCDREYLVAPLWLL
                     EKQKMLKLLEEKMEEKNVYQGKNYKKRS"
     gene            11149..12996
                     /locus_tag="PLF90_03350"
     CDS             11149..12996
                     /locus_tag="PLF90_03350"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOK56355.1"
                     /translation="MKVIILCAGYATRMYPLTLVKSKNLLQIGGKVMVEHIIEKLNKI
                     PEIDKIVIISNDKFYSDFNLWAEDKKNIVVLNDGSNSDKERLGAIGDIEFAVDRLEDD
                     EFLIIAGDNLFEFDLLDFVNYGRKNGITVGIKRVEDINLIKKYSQVVLDEKNRITYFE
                     EKPQNPKTKDAAICVYYFPKRTLKKYLDEYLNSGNNPDQAGLFIQYLHKKVDVYGYVF
                     EDNWFDIGDINQFHSANFYILSKKMREIFGIGEEPLFFIAPGRVNIIGEHTDYNGGFV
                     MPFAVDRYIYLAIRKNNKKVINFFSLQFKNAVSVEIGNINKTNTWADYPLGVFKILSE
                     YGYKIDGVDLVFESNIPIGGGLSSSGAIEVITLTALNNLFNLNIEKEKIPFMCQKAEN
                     EFVGARCGIMDQFIITMAKKNNAILLNCKNHNYQYIPVNIGDYYFLISSTNVSHSIAS
                     GEYNKRRAECEEGLKILKKYIDIETISDIKIDEFEKYKDKLPEVVRKRVLHVIKENER
                     TEKASKCLIDGNIEELGKLLNESHISLRDLYEVSCKEVEIMRDESLKIEGVIGTRITG
                     GGFGGCLISIIHKDSVDKYIKEVGKNYKEKTGIIPEFHICQIVSGCSSF"
     gene            13086..14099
                     /locus_tag="PLF90_03355"
     CDS             13086..14099
                     /locus_tag="PLF90_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK56356.1"
                     /translation="MKPITKMVTIYEIAKKAGVSPATVSRVLNNGPVKSSTKEKILKI
                     IEKENFVPDNRAVYLKKINTRKIGLIIPDILNPIYSLAVKVIHDYLKEKDYHLILGNS
                     YGNVDEEKDILELFERERVAGVIIGTCEGEDDSSLIPIFDRMLKSGIKIVFTGKKNYG
                     LKVDVISVNNEIGTYKITNYLIKTGRKKIGFISGDRNLRATEERLSGYIKALKEKGIG
                     INKELIICEGEYKMENGRKFAKELISKVDAILCGNDLMAIGAIKAVEESGLKVPDDIG
                     ICGFDDIFISYLIKPSLTTVHQPIEKISEIACERLIGWIEGKIKKEEEILIEPEIIVR
                     ESA"
     gene            complement(14105..14383)
                     /locus_tag="PLF90_03360"
     CDS             complement(14105..14383)
                     /locus_tag="PLF90_03360"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOK56357.1"
                     /translation="MEYKVIILPTAISDIEKLSKSIKTGILKKIKYLAENADEIIHHH
                     LKNMPDDLKGLCRIKYGHYRILYWIYRNEKIIKIYKVSHRSIIYKKLI"
     gene            complement(14365..14565)
                     /locus_tag="PLF90_03365"
     CDS             complement(14365..14565)
                     /locus_tag="PLF90_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56358.1"
                     /translation="MRKNEELIVLPKNVLLTAETLEDIEDWLLSRNKKFIEELRRIRL
                     EEDLKGKGEELEKICKKWNIKL"
     gene            14619..16112
                     /locus_tag="PLF90_03370"
     CDS             14619..16112
                     /locus_tag="PLF90_03370"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOK56359.1"
                     /translation="MKKEIYNKIYGCFLGKSIGGSIGGPFEGKKEFLEIPYKLPEKIL
                     ANDDLDLQIVWFDILRKKGIKISSEDLVKGWENIDYPFDEYGVAKANLKVGLRPPLSG
                     IYNNWFCECMGAPIRSEIWACIFPARPEIAGYYSYLDASVDHWYDGAYGEIFLSVIES
                     LAFVENDIEKLILESCRFLPEDSKVRKVSEMVLRLYKEGKELKETRDEVIKNFGHYNF
                     THCVQNIGFIISGLLYGNGDFLKTIIESVKCGYDTDCTGATSGAIIGIILGKEEIEKQ
                     IKTEIDKRIVPGWGIKNIYIPKDIEEFTEEILKVIEIAEKEKDLPGIEKPFKLPEIPE
                     FEPPLKIKFGVSDVFELKEAEDIEKKIIEDRYYGFKEFVSDTIYLPLDKFFGDKKGLL
                     FLKTDIKFDKKQKIKIYPATTDGVKLWLNKKLILSHHNHNKEFIPAPHRPSSPFVYVE
                     IKEGKNEILIEIFKCKEKIEFALILADERNHLIYPITEPGLPMKETT"
     gene            16214..16972
                     /locus_tag="PLF90_03375"
     CDS             16214..16972
                     /locus_tag="PLF90_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOK56360.1"
                     /translation="MKKIRKRKGFTLIELLVVVAIIAILAAMLLPALSKARERARQAT
                     CMNNLKQIGLALLMYVEDYEYVPSGDSFETGYAPRFWFQKLNYYISINKKEGHNYPHP
                     VWRCPSNRKHAFSSTWISYGCNVNVMYSTNSVVNQTVRTPPKYARIRRPSGVIAVADS
                     NGDGYYDMFVQGIGPAYVYSGSGSETYAPGFRHNGGCNVLFFDGHVEWKKEQEVFMLK
                     TGGWSWGTPSTTPEWLRLLWGANWDGCNPPYYMR"
     gene            16994..17875
                     /locus_tag="PLF90_03380"
     CDS             16994..17875
                     /locus_tag="PLF90_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOK56361.1"
                     /translation="MQRELGIYIDHMCKDFERFIKNISEAGFGYICTQDSIDYLNFSK
                     EDRIKRIFETKEIGEKYGLKFHAHHSCSVLLPSLDIKESANYLKNLIDELKNWDLSFY
                     VLHARSIKDEPKPWKIIEYIGKDKFDKISVEVLKDVCDYAYKYNISIALENLPYPYVN
                     RVKYILEIIEKVERENLGICFDSGHSNISTESVYEELESIGTKLFTTHFHDNLGNKDK
                     INIDDENISKYDLHLVPGLGIINWIKINKILDKIDYRYPVVFEGIKGIEREEDLFEIT
                     VKLWRSFEKLSMEVKDD"
BASE COUNT         6151 a         2585 c         2519 g         6656 t
ORIGIN      
        1 tttctgataa ttttttcagt cgcaatcccc tttaaagcag gtcagaaatt ctaacctgaa
       61 aaaagaactt gaataacggc aagtcaggga caagtcgcaa tcccctttaa agcaggtcag
      121 aaattctaac tttatctata aaggattgta tatgttgctt tctggaactt gtcgcaatcc
      181 cctttaaagc aggtcagaaa ttctaaccta ttaaaacaat atcttcaatt tcattaacta
      241 ctttaatgtc gcaatcccct ttaaagcagg tcagaaattc taaccgcggg ctttcaaaga
      301 gcaagtatat caataatttt aatatccttt tccgcaaaac cactattttt caacttaaaa
      361 tttttataat ataaatacct tattttgtca agtccattgt ctctcaataa atcccaactt
      421 gcgctaaccc ccccctactt cctgcttaaa aaaacatttg cgctaaaatt tatttctttc
      481 tataaaccat tcccaaaaag gtataaattt tactcttcct tttataccaa accattttat
      541 ttcttcttcg gctttatatt cgttattcaa tattatcaga ttttcacatc tcaattcttc
      601 ttttcctttc aaaagtgccc ttatttctct tttttttgtt tttatatctg aaatttcata
      661 agcaacctgt atcaatgaaa ttgtgtctgt tcctttcctc actacaaaat caatctcttc
      721 tcctttttca tttttccaat aatatagttt tattttccct tccatttcat atcttttaag
      781 ttcaactgcc acagcatttt catataaaaa tccagtatct tctgttgctt taagcgaaca
      841 ggtataaata aatccattgt ctatacaata tattttctta tttgaagtta tcatttgttt
      901 taatttgaaa gaaaatttgt taactctgaa aatcaaaaaa ccttcctcaa ggtaatcaat
      961 gtatctctca atagtatgaa cacttcttgc accagttatt tttgataatg tattgtatga
     1021 aaattctttg cctgtgtttg atatgagata aatagcaagg ttttctataa catctggttt
     1081 tcttattctg tatcttttta ctatatcttt gtaaatcaat gaattaaaaa gcatactcaa
     1141 ataatcctta aaatcaattt ttttaatatg tggttcagga aatccaccaa aatttatata
     1201 ctgaattaat ttacttttta tttcactcgt cgtaagttct tttccctcaa tccttaaaaa
     1261 ttcctgaaat gaaaatggaa aaacaaacac tgaaatatgt cttcctgtta aatgtgtgga
     1321 aaggtctcta cccaaaagtt ttgaattact tcctgtaaga ataatgttaa atccttctct
     1381 ctgtaatcta ttaacaaata tttcccaatt tccaatgttc tgtatttcat caaaaaaaag
     1441 tatctttggc tttctgtaaa caatttttaa agcatttatc aggtcatcat aattcttaat
     1501 gttgattatt ctttcatcct caaagttaat gtatccaaaa tcgccttttt cttttaaaaa
     1561 atgtatgcca aaaaatgatt tgccagccct cctcggtcca attataacct taattaaatc
     1621 attttcaaac ttccttttgt ctatatccct ttcaataaac ctttcctcaa acttcctctc
     1681 tatttctctc ttttgtaaaa gtaatatatc tttaattttt tccatcttaa tgcacaaatt
     1741 ttactatttt tgtgtattat attttaatat ataacaaaaa tctgtcaaat ttgataactt
     1801 gaaatggctg accttgtttt cgtttttaaa ttatataaaa atccatgtct tttgtaatct
     1861 ctcctatgcc aattatctct acttttttta aacaaccttc acatattata taaaatctta
     1921 tgctatcatt ctctttatct attatctcac ctgtcttttc ttttatcttt tttaattgct
     1981 catcatctat aatacattca aaaacactat actgaacgcg tgtgccataa ttttcaagta
     2041 tctgacttac cttaaatctt tttttatcat cctgaatatc ataacttatt accacaaaca
     2101 ttttatttca actcaaaagg taaatattca cagttttcca gtattgtttt ctcaaatttc
     2161 cttatctggt gttgaataag ttttctgtaa ttcattgtct ggtcttcata atttatttca
     2221 gttaaaattc ttctctcata ctgttttaaa tattctttta tagatgcctc atttaaataa
     2281 atttttcctt cctcttcttc acgaaaacca tctcccttta ttatctcttt gttaattatt
     2341 tcaagtacca atctatcaat aattacctgt ctgaattctt ccattaaatc aagagcaagt
     2401 gcaggtcttc catactctac tgaatgcata aatccaagat aagggtcaaa acctattgcc
     2461 tgtaaaattg aaaatacctc acttgttaaa atagaataac caaaacttaa aagtgaattt
     2521 acagggtctt ttggatttct cggtactctt ttttcaaatt caaagttttt aagtatcatc
     2581 tttccaaaac aatcaaaata aattgcagaa catctacctt caagtccaac tatagatgaa
     2641 acagaatttt ttcttttaag ttcttcaagg ttttcttcaa tttcttttat ttcacctgaa
     2701 aaatcaattt ctggatgatt tctctggtat ttctgtagaa aattttttat gttgttaatt
     2761 ttgccttcta caaacttttt tgagatttca actctaaatt catctgaatt atatttttca
     2821 aactgtttta ttcttaaaag aacatttttt gattcaaccg gctgaagttt tcctataaat
     2881 ttaccataga ttgtaaaaaa ggaagtgtca atgcctgatg taagtaaaaa ttttaatgcc
     2941 tgagttgtga tttgtatatt cccaaaaata aaaactttat caactttaaa ttcaggaatt
     3001 tcaaatataa cactgtcttc tttttcaatt acaaatcttt tatcttcttt ctttaatttt
     3061 gcaccctgtt ctgtaatata taatgtcgcc aatttcagaa gttattaggc aattggaaat
     3121 actcaatttt tattttaatt tggttgcaac aatcatttat aaagtccttt ataaatttta
     3181 aaattttctt ccaatcatct ttatttacag gtctatctcc atgtgcaagt tttgaagtgt
     3241 ttctgatatt caaaatattt ttaaattttt tttctttttc tctataaatt tttccaattt
     3301 cgtctccaag ttcatctaac aattcatatg aatcaacaag cccaattttt attttccctt
     3361 ctttatcttt tttaatttcc catttttctt tatttttaac tttttctata tcaacacttg
     3421 atgtctcaat accatattta ttttttaatc ttttttgtgc aaaaagttct aatgctctat
     3481 ataatcttgc tgtagcattg tcataaaaag aattatgtgc ctgtctttca gcatttgcca
     3541 ctaaatcaaa aacagattcg tatccagtta ttttttcaca aatctttaat aaataattta
     3601 aatgatcttt aaatctgcca ccatagtttt taagtattga aagagatttg tcgtattcaa
     3661 aaatgtccca ataataaaaa caggtgcata attcacattt ctctataatt tttttctgtg
     3721 tttctctatc aaaagttgag tacgttaaaa tattttcaag tattactttt gcaggataat
     3781 ataaatatcg ggatataaat tcattaacaa tttgaagaag gaaatcaacc cttgcagaat
     3841 ttacattttc aaggacaggg aaactcaact ccttaatttt tataatatct tctctttttc
     3901 ctttgacaat agaaggtttt gttttgaatt caaaagcagt aagaatagcc agaacacttg
     3961 acattgtttt agttcctcct gtaaaatcac agataatctc atctccttct tttaaggctt
     4021 tttctattgt ttgttttgtt ttttcataaa cttcgttaaa atcatcaggg tcattaagta
     4081 ccactttttc gtaagattca gaatattctg cttgttctat aattggtggg tatttttctc
     4141 ctttttcaat tatttctcca cactttttac attttatttc cttttttcgt aaacatggtt
     4201 tttcatttat aactctacca tttttatctt taccatcaat agtaatgctt gatgcaacct
     4261 caggtttgcc accagaacaa ataaaatata taaaatcagg attatatcct tttatagcag
     4321 atacaacagg ttcatcgctt ccaccaactg tgattataag aattcttctg ctcatattat
     4381 ttgaatagaa acccagccca ttgaaatggc accagatatt aattttctcg ttttaggttt
     4441 ttggacttta taaaaaattt taagtttttc agaaagtatt aaaaaggaag tagcccaagc
     4501 agttgaaccc tggcctaatc ttaataaata tccatccttt ggtggttcag gtaatataat
     4561 aattttccca gttgcatatt ctttttcttt tttataaact tctctataaa atctatcagc
     4621 catacttaaa atttcttctt ctttgaattt acaatcatct gttgttttta tttctgtctc
     4681 aaattcagta tttattttta atgtctcata taaaattggg agtttgtttt gtgttttagc
     4741 accaataagt tttgaaagag aaagttctaa tgattcttct ggttttttaa aatttgaatc
     4801 ccttacaatt aaatattgtg caaatttccc tctatcatat tttcctgtga tttcagataa
     4861 aacttctgca agatggttct caaaatttgt aaattttttt atatttttct tatacaaaac
     4921 ttcttccatc aatcctgata aaatacttcc ttttaaagaa ctgccaggaa tataaacact
     4981 tccacaagat tttataaact cttttacttc aattggattg aatttttcag ttgtttttga
     5041 tatatctatt tggtatagag tacatgaaag aagtaaatca agtggtaaaa tattttttat
     5101 atatctttca ctttgtgcca tatttaaaaa tttatcttta aattttgaaa attcaggtga
     5161 ttgaaaaagt gcatccatat taatcctgat gaatttattt tcttcaagca catattcaag
     5221 aggacttatt tcttttccag aaccaatatg gactggactt aaaactttta ttttaatttt
     5281 cattttgaac ctctatttta cagggtaaag aaaatgcaaa accatatcta taaacattat
     5341 ggctttggaa tatttcagta ggtgttacat ctgctatttt tccttttttc attgatggaa
     5401 aaacagaacc ttcaataaac attctgattg atttttttct taaagatttt gtgtatggag
     5461 aaaagatata tccttttctt tcaattatct cataataacc atctttaagg tcttttaatt
     5521 cattttcctg tggtaaataa agtgaaagaa ttatacaacc aaaagattca gaaggagttt
     5581 gaatttcaaa tttatcaatt gtatcaattg taaaatttcc tttcccaaca cttctatctc
     5641 ctccaattcc ttcgtctccc attaatctta atgttgcaaa aaatttcttt tcaaaatcag
     5701 gttctttaaa atctactacg aaaaataaac cacttccttc ttcaaaaaat acttctccaa
     5761 aatgaaaata gttttctcca ggagttgtat ttaatctgct taaaccaatt ctcggatttt
     5821 caattatatt gtaatgtgtt tttccatctt tttcaggtat tgtgctgtaa tttagataaa
     5881 tatcttcaag tttttctcca tttaacaatt tttcaaatcc atccttttct atatacttaa
     5941 tttttttaat tactttttct ttcggcaatt gatttaaagg tacagggaaa tataattttt
     6001 ctttactgta tggaaacatt gaagaaatta aaaatacagg attattgttt ctgaattttt
     6061 ctataagttg gttcagttcg ttttctccgt aaagcaaaag gtagttataa cagaaagcag
     6121 aaaaaagtgt atctgaatgt attatttctt ctgttgcttc taaaaatcct tctctttctc
     6181 ctgaatgaaa agaagattta aatttaatct ttacaatttt cattttcttt cccttttagg
     6241 tgatgttatt ttggatatta gagaagttct ttacaatatc tttcggagta gcatacccat
     6301 cattgatttt gtacacattg atattcccac tttcataatc ttctttttta ttccagtaaa
     6361 tttcaatatt ctcaaattta atttttccat accctcttga acccatacca ccaagataat
     6421 catcttcaag caattccata gattcaaaaa caactttaag caaatttttg tcactttctt
     6481 caaaaatatt aaagaccatc tccataggtc taaaaactgc tcctgctggc accctgtcaa
     6541 tggttcttaa gcttttttct aaagcagtac cttttaacct atcaattgct gtttccattt
     6601 ttatttctgt ccattcaagc tccatatttc ttctcatttc ttctgtgatt gatttttcat
     6661 ctaaaaaaat atctcttaca attaatctcg ttcctactgg aatccctaat aatttatcag
     6721 aacttaaaat tccccaaact ttgcatattt tacactttaa atattcttca ggagcatcac
     6781 attcatgcca atatggctct tctttttttg aatttttaac atttctcgtt ttatttagtt
     6841 ttactttctc atttttttct gcaagcattc ttaatttccc ttttaaagaa gaaccgggga
     6901 tataaggcct tccaaaagca tcagtaatta ctggttggtc tacccctcca attttcaaac
     6961 ctgtagtggc cccagcaatt cttaatccag taataacttt aatatctcct ttaatcaata
     7021 ttttccctaa caaatttact tttattttat tttcatttaa cattttgccc tccttttaat
     7081 cctttacttt actataaaat ctatgataac ctacaattgc ctcaaaaaaa tatctgaaat
     7141 tttcaaattt tttataatca tctccaacca gttgaagtgc ataatcaatt atttccttta
     7201 attccttcaa agcaaatgac ttattatttc ttcccgcaat ataagccaat tttggtctta
     7261 aaatttgatg cttctgtgat tttatatctt cttctttcat cctttgaaca tcatccagta
     7321 tatttctaat ttgtgaagta gttaattttt cttccttctc tcgtggattt tggggcgcgt
     7381 atttctcacc aatctcctgg gcatatttat ttaatatctc aaaatctcca tttattatct
     7441 tctcaataat ttctttgttt atattagtca tagccacctc cttttaaact tttttcctca
     7501 ttttctccaa aaaatcttta tcaaaagtca tcaaaatttt tccttcttcc ctcgcaattt
     7561 caaaaaactt ccatttttaa ttgtatcaat tctcaatatc tattcctttt tcctttaact
     7621 ttttaatcaa atccagatga aaattttcat ttgtcaaaat ttcatattta ataaactttt
     7681 tcatctgtta aaattaaaag tgaatattca atcgctccaa gtaatctaac tttctttcac
     7741 cctccttttc ttgttaatag ttctgcccat cttactgcta catttaattt aggttgaata
     7801 ttaaaatcag ttataaatgt ttccctgatt ttatcaatat cattttcgta atctttatgt
     7861 ctttccataa atcttttcaa tgcgtaaaaa attctccaga ttctcacaaa tggtattttt
     7921 cccctctctt ttagttcttt ttctttatat cctccatata aaattgtcaa aatagccctt
     7981 gatacttttg gttttccatc ttctttcaaa agttcataga gtttattctt tacttccttt
     8041 atttttacaa attcttcatc ccattttatt ggttcaaaaa atgtgatttt atttttttcg
     8101 cctttttttg ctttttcttc ttcctctcct gctttttttg ctgcctgata aacaggaaat
     8161 ttcttcgaag gagcaatgta tataccacct gaaattgtta tcttttcatg attacacaca
     8221 tattttctaa agtttctcca tatttcctca gcaatttccg gcaaatcact ccatggcgca
     8281 attataaata aatcatctcc tcctgaatat acaacacagc atttttcttt atattctttt
     8341 tcaacaatat tccgaattcc aagagaaaag aaaagtgaaa atgtataact taacatacta
     8401 tattttgaaa ttgttgttat atctttccct tcttcatcaa taaaacctga accaaaaatt
     8461 aaaccaagat tgtcaacatc cattcttaaa gcaccccatc tttcaatacc ttctgccttt
     8521 ttagcaatat cttcaagagt tccttctgga ctataaattg cttcatatct aaaagcatca
     8581 caatttactt tcagaaaatc agagttgttt aaataaattt ttataccccc tatacttttt
     8641 tcaaaactta ttttataacc cagtccttca aatatttcat cccatttatt aacttcttct
     8701 tttatctctt ttttattaac tttttttatc tccataatca cattttcttt ctttatttct
     8761 attgcaagtt tttcaaaact ttcacagagt tcacatttat cttttttttc tatttctctc
     8821 ccacagattt cgcaaccacc catttctttt tccacttttt ccggatatgg tttaaagaga
     8881 ttttcggata ttaaatattt aaattttttt cttttttcaa ttgccaattt tcttccaagt
     8941 tcagataata aatcgccaaa attttcaata aaattccagt aaggaaattt aatataagag
     9001 ataagaaatc caagtttgcc ctgatgtgct ttaaagatta ctttatctat ttcttctgtc
     9061 agtttttcca atcttttttc tgcatctgaa agatttggaa gtaaaattat aaagtttcca
     9121 cctccaagat atataagatt tgcttttgtc agattgaatt cgtcaagtat tttataagca
     9181 ataatttcag agattaattg aagataaaaa gaccttcctc ttaaaccctt taatgcatgt
     9241 tcacttgata cagaataaat aaagtcctga attcctgaaa tgtctccttt cagaagtata
     9301 aaatcttttc tattaagttt tccataatct atctcttcta ttaattcacc agtccttctt
     9361 ttttcaaaaa tttttttaag ttcttcaatc agtttatcaa gtgtttcttt tgcaatatta
     9421 aggtcatata aacaggtagc aatagcacat gtcagtttta agtgatggaa cagagaaatt
     9481 tcgggtttat cagtatatgc agaggatggt ataaaaagag tatatttttc aagaagagag
     9541 aaaatctgag tgaatatttt ttcaaaattt gatggatagt ttaaatactg acattcatct
     9601 ttaaattctt cccataactt tttaaaatta tagttatctg tgattgcgtc ttttttttca
     9661 acaggcacca atttttcaag attattatca attttaacag gtctattgta taattcttct
     9721 ggtttataaa tttcaccgtt tttttcatct ttaataagaa gttgtgaaaa tattgaaatt
     9781 attggttctt ttttgactac ccctttttcg ctttcgtccc tttctcttct ttctccactt
     9841 gatagataat cagcaagttg tattattttt ggaagttttt tatctccttt aaaactatct
     9901 ggtttatggt gtgaaagtac aagattttct ataggtgatt ctttatcttt gaatatatct
     9961 tttacaaatt gacctgaata taaaagatga gagtagccat aatcagtcgg acaataaaat
    10021 gttcttaaat ttttatcata ttctaacctt aacttttctt cggcacggtg ggaaaatttt
    10081 cctatatcgt ggagtaaact acctaatatt aaactttccc tttcattcat acaaaatttc
    10141 ctattttttt taatttagat taaatttcta attttgtcaa gaagatttta ggattttaag
    10201 gtttttgatt agaaggtgta aaataaataa atggaacttt ataagaaatt ttcagattat
    10261 ttgaggaaaa ggtttggttg cagggtacat aaaataagtg tttatgctgg tttttcctgt
    10321 ccgaatattg atggaagatt aagtgataaa ggatgtattt actgtaataa ttatgctttc
    10381 agtccatctt taagagaaat tttgccactt gaagaacaga taaaaaaagg aattgaatat
    10441 ggtaaaagaa ggtttaaggc agaaaaattt atagtttatt tccagccata ttcaaatact
    10501 tatgcacctt tagaaatttt aaaagaaaaa tatgatgttg ttaaaaaatt tcctgaagtt
    10561 gttggaattt caatagggac aagacctgac tgtatagatg aggaaaaact tgatttgatt
    10621 gagagttaca gcagtaatta tgaggtctgg attgaatatg gacttcagag tattcatgat
    10681 aaaacattaa agattataaa caggaaccat acttattcag attttttaaa agtttacagg
    10741 atgacaagaa aaagaaatgt gaagatatgt gctcatgtaa taattggact gccttatgaa
    10801 acaaaagagg aaattattga aactggtaaa gagtgtggaa tattagaact tgatggaata
    10861 aaaatacatc caattcatat tttaaaaaat acagaacttg aaaaatggta taaggaaggg
    10921 aaatttaagg ttatggatat ggaagaatat attgatttgt taattgattt tctttcttat
    10981 ctttatcctg aaactgtaat tcagagattg accgctgatt gtgatagaga atatcttgtt
    11041 gctcctttat ggttgcttga aaaacagaaa atgttaaaat tattggaaga aaaaatggaa
    11101 gagaaaaatg tttatcaggg aaaaaattat aaaaaaagga gttgaaaaat gaaggtaatt
    11161 attttatgtg ctggttatgc aacaaggatg tatccattaa cacttgttaa atcaaaaaat
    11221 cttctacaaa taggtggaaa ggttatggtt gagcatatca tagaaaaact taataaaatt
    11281 cctgaaatag ataaaattgt aattatctca aatgataagt tttacagtga ttttaattta
    11341 tgggctgaag acaagaaaaa tatagttgtt ttaaatgatg gttcaaattc agataaagaa
    11401 agattgggag caataggaga tatagagttt gcagttgata gattggaaga tgatgaattt
    11461 ttaataattg caggggataa tctttttgag tttgatttat tagattttgt gaattatgga
    11521 aggaaaaatg gtataactgt tggaattaaa agggtagagg atataaacct tataaaaaaa
    11581 tacagtcagg ttgttcttga tgaaaaaaac agaataacat actttgagga aaaaccacag
    11641 aacccaaaaa caaaagatgc tgcaatatgt gtttattatt ttccaaaaag gactttaaaa
    11701 aaatatcttg atgaatactt aaatagcgga aataatcctg accaggcagg tttattcatt
    11761 caatatctgc ataaaaaagt tgatgtttat ggatatgttt ttgaggataa ctggtttgat
    11821 ataggagata taaatcagtt tcacagtgca aatttttata ttttaagtaa gaagatgaga
    11881 gaaatttttg gaattggtga agaaccatta ttttttattg ctcctggaag ggtaaatata
    11941 attggagagc atactgatta taatggtgga tttgttatgc cttttgctgt tgacaggtat
    12001 atatatcttg caataaggaa gaataataaa aaagttataa attttttctc acttcagttt
    12061 aaaaatgctg tttcagttga gataggaaat ataaataaga caaatacatg ggctgattat
    12121 cctcttggag tttttaaaat tttaagtgaa tatggatata agattgatgg agtggacctt
    12181 gtttttgaaa gtaatattcc aataggaggg ggactttctt cttcaggagc aattgaggtt
    12241 ataactttaa ccgccttgaa taatttattt aatttaaata ttgaaaaaga aaaaatacct
    12301 tttatgtgtc agaaggcgga aaatgagttt gtaggagcaa gatgtggaat aatggaccag
    12361 tttataataa caatggctaa aaaaaataat gcaatacttt taaactgtaa aaatcacaat
    12421 tatcaatata ttcctgttaa cattggagat tactattttt tgatttcttc aacaaatgta
    12481 tctcattcaa ttgcatctgg agaatacaat aaaagaaggg cagagtgtga agaaggttta
    12541 aaaatattaa aaaaatacat tgatatagaa acaatttcag atattaaaat tgatgaattt
    12601 gaaaagtata aggataaact tccagaagtt gtgagaaaaa gggtcttgca tgtaataaaa
    12661 gaaaatgaga gaacagaaaa ggcaagcaag tgtttaatag atgggaatat tgaagaactc
    12721 ggaaaattgc tgaatgaatc acatatatca ttgagagacc tttatgaagt gagttgtaaa
    12781 gaggttgaaa ttatgaggga tgagtcattg aaaatagaag gagttattgg tacaaggatt
    12841 acaggtggtg gatttggagg ttgtttgatt tctattatac ataaggactc tgttgataaa
    12901 tatattaaag aagttgggaa aaattataaa gagaaaacag gtattattcc tgaatttcac
    12961 atctgccaaa tagtaagtgg ttgctcctcc ttttaattcc gaacccggtt cggaaaaatt
    13021 tgtaatcaat ttcaaaaaat ttgacaaaaa attttttatt ggtataataa taaaaagaaa
    13081 taaaaatgaa accaattaca aaaatggtaa caatatatga aatagcaaaa aaggcaggtg
    13141 tatcaccagc gactgtatca agggttttaa ataatggtcc tgtaaaaagt tctacaaaag
    13201 aaaaaatttt aaagataata gaaaaagaga attttgttcc agacaataga gcagtatatc
    13261 taaaaaaaat aaatacaaga aaaattggac ttattattcc tgatattctc aatccaatat
    13321 attctcttgc tgttaaagtc attcatgatt atttaaaaga aaaagattac catttaattt
    13381 taggaaattc ttatggaaat gtggatgagg aaaaggatat tttagaacta tttgaaagag
    13441 aaagagttgc aggagttatt attgggactt gtgaaggaga ggatgatagt tctttaatac
    13501 caatttttga taggatgtta aaaagtggaa taaaaattgt atttacagga aagaaaaatt
    13561 atggacttaa agtagatgtt ataagtgtaa ataatgaaat tggaacatat aagataacga
    13621 attaccttat taaaacagga agaaagaaaa ttgggtttat aagcggagac agaaatttaa
    13681 gagcaactga agaaagatta agtggatata ttaaagcatt aaaggaaaaa gggataggga
    13741 taaataagga actaataata tgtgaaggtg aatataaaat ggaaaatgga aggaaatttg
    13801 caaaggaatt gataagtaaa gttgatgcaa ttttatgtgg aaatgattta atggcaatag
    13861 gtgcaataaa ggcagtagaa gaaagtggtt taaaagttcc tgatgatatt ggtatttgtg
    13921 gttttgatga tatatttatt tcttatttga ttaaaccatc tttaacaaca gttcatcagc
    13981 caatagagaa aatatcagaa atagcgtgtg aaaggttaat aggatggata gaaggaaaaa
    14041 taaaaaaaga agaggaaata cttatagaac cggagataat tgttagggag agtgcatgaa
    14101 aaaattaaat aagtttttta taaattatgg acctatgaga gaccttataa attttaataa
    14161 ttttctcatt cctataaatc cagtaaagaa ttctgtaatg accatattta attctacaaa
    14221 gtcctttaag gtcatcaggc atatttttaa ggtggtgatg aataatttca tctgcatttt
    14281 ctgcaagata tttaatcttt ttcaaaatac ctgtttttat agatttagaa agtttttcaa
    14341 tatcagaaat agcggtgggc aaaattataa ctttatattc catttcttac aaattttttc
    14401 aagttcttca cctttacctt ttaaatcctc ttctaatctt attcttctca attcttcaat
    14461 aaatttttta tttctggaaa gaagccaatc ttcaatatct tcaagggttt ctgcagtcaa
    14521 gagaacattt ttaggtaaaa caattaattc ttcatttttt ctcataataa ctcctttttt
    14581 gtttattata ccttatttat ttttaaaagg aaataaagat gaaaaaagaa atttataaca
    14641 aaatttatgg gtgtttctta ggaaaaagta taggaggaag tataggtgga ccttttgaag
    14701 gaaagaaaga atttttagaa ataccatata aacttcctga aaaaatactt gcaaatgatg
    14761 accttgattt acaaattgtc tggtttgata tattgagaaa aaaagggata aaaataagtt
    14821 cagaagattt agtgaaggga tgggaaaata ttgattatcc ttttgatgaa tatggagtag
    14881 caaaagcaaa tttaaaagtt ggtttaagac caccattaag tggaatttat aataactggt
    14941 tctgtgaatg tatgggagct ccaataagaa gtgaaatatg ggcatgcata tttccagcaa
    15001 gaccagaaat tgctggatat tatagttatc ttgatgcctc agttgaccac tggtatgatg
    15061 gtgcttatgg agagatattt ttatcagtaa ttgaatcact tgcctttgtt gaaaatgata
    15121 tagaaaagtt aatattggaa agctgtaggt ttttaccaga agattcaaag gtgagaaaag
    15181 taagtgaaat ggttttgaga ttatataaag aagggaaaga attaaaagag acaagggatg
    15241 aagtaataaa gaattttggt cattataatt ttacccattg tgttcagaat ataggattta
    15301 taatatcagg acttttatat ggaaatggcg attttttaaa aacaataatt gagtcagtaa
    15361 aatgtggata tgatactgat tgtacaggag caacatctgg agcaataatt ggaataatac
    15421 ttggaaaaga agagatagaa aaacagataa aaacagaaat agataagaga attgtacctg
    15481 gctggggaat aaaaaatatt tatattccaa aagatattga agaatttact gaagaaattt
    15541 taaaagttat agaaattgca gaaaaggaaa aagatttacc aggaatagaa aaaccattta
    15601 aacttcctga aatacctgaa tttgaaccac ctttgaaaat aaaatttggg gtatcagatg
    15661 tatttgagtt aaaagaagca gaagatattg aaaaaaagat tattgaagac aggtattacg
    15721 gatttaaaga gtttgtttct gatacaattt atttacctct ggataaattt tttggtgata
    15781 agaaaggttt attattttta aagacagata ttaaatttga taaaaagcag aaaattaaaa
    15841 tatatccagc aacaacagat ggagtaaaat tatggttaaa taaaaaactt atcctttcac
    15901 atcacaatca caataaagaa tttataccag cacctcacag accaagttca ccatttgtat
    15961 atgtagagat aaaagaaggg aaaaatgaaa ttttaataga aatttttaaa tgtaaagaga
    16021 agattgaatt tgccttaata cttgccgatg aaagaaacca cttaatatat ccaattaccg
    16081 aaccgggttt gccgatgaaa gaaaccactt aatatatcca attaccgaac cgggttcgga
    16141 aggaggtgat gtgagatgag ggaaagttat aaaaattgaa aagaaaaaaa ttagaagaaa
    16201 ggaggtaaat aaaatgaaaa aaataagaaa aagaaaaggc tttacactta ttgagttgct
    16261 tgtagtagtt gcgattatag ctatccttgc agcaatgtta ttaccggcac tatcaaaagc
    16321 aagggaaaga gcaagacaag caacctgtat gaacaattta aaacagatag gacttgcttt
    16381 attaatgtat gttgaagatt atgagtatgt tccttcagga gatagttttg aaacaggtta
    16441 tgcaccaagg ttctggttcc aaaagttgaa ttattatatc tctataaaca agaaagaagg
    16501 acacaactac cctcatcctg tttggagatg cccgagcaac agaaaacatg cattttcatc
    16561 cacatggatt tcctatggat gtaatgtaaa tgttatgtat tctactaatt cagttgttaa
    16621 tcagacagtt aggacaccac caaaatatgc aagaataaga agaccatcag gagtaattgc
    16681 agttgctgat tcaaatggag acggctatta tgatatgttt gttcaaggaa ttggacctgc
    16741 ttatgtatat tctggttctg ggtcagagac atatgcgcct ggatttagac acaatggagg
    16801 atgtaatgtt ttattttttg atgggcacgt tgagtggaaa aaggagcagg aagtatttat
    16861 gttgaaaaca gggggatggt cctggggaac accttcaact actcccgaat ggttgagatt
    16921 attatgggga gcaaactggg atggttgtaa tccaccttat tatatgagat aaatttgaaa
    16981 agaaaggaaa attatgcaga gagaacttgg aatttacatt gaccatatgt gtaaagattt
    17041 tgaaaggttt attaagaata tttctgaagc aggttttggt tatatatgca cacaagattc
    17101 tatagattac cttaattttt caaaggagga tagaataaaa aggatttttg aaacaaagga
    17161 aataggtgaa aaatatggat taaaatttca tgcccatcat agttgttcag ttcttttacc
    17221 ttctttggat attaaggaat ctgcaaatta tttaaaaaat ttaattgatg aattgaaaaa
    17281 ttgggatttg tctttttatg ttttacatgc tcgttcaatt aaagatgagc caaaaccatg
    17341 gaaaataatt gaatatattg gtaaagataa atttgataaa attagcgtag aagttttaaa
    17401 agatgtatgt gattatgcat ataaatataa tatatcaatt gcccttgaaa atttaccata
    17461 tccctatgta aacagagtta aatatatttt ggaaataata gaaaaggttg agagagaaaa
    17521 tcttggaata tgttttgatt cagggcacag caatatttct acagaatcag tttatgaaga
    17581 acttgagagt ataggtacta aactttttac aacacatttt catgataatt taggcaataa
    17641 ggataaaata aatattgatg acgaaaacat ttcaaaatat gatttacatt tggtcccagg
    17701 gcttgggata attaactgga taaagataaa taaaatactt gataaaattg attacagata
    17761 tcctgttgta tttgaaggaa ttaagggcat agaaagggaa gaagatttgt ttgaaataac
    17821 agttaaatta tggaggagtt ttgaaaaatt aagtatggag gtaaaagatg attaaaaagt
    17881 attttatttt aagtttaatt tttttgtttg t
//
LOCUS       DAOSAT010000025        17898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2183_length_17898_cov_5.668946, whole genome shotgun sequence.
ACCESSION   DAOSAT010000025 DAOSAT010000000
VERSION     DAOSAT010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2183_length_17898_cov_5.668946"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1581)
                     /gene="serA"
                     /locus_tag="PLF90_03385"
     CDS             complement(7..1581)
                     /gene="serA"
                     /locus_tag="PLF90_03385"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOK56362.1"
                     /translation="MEKVKVLITEPFEKEGKDILVNEGFEVKEAGKVSSEELIDMITP
                     YDVLIVRSGTKVTKEVIEKGEKLKIIGRAGVGLDNIDIEAATLNGVIVMNAPEGNTIS
                     AAEHTIGLIFSLARNIPAANQTLKNGKWEKKKFLGTELYGKTLGIIGLGRIGKRVASM
                     AKGIGMKVVGYDPYVKEEVIRDLGVTMLNFEGVLKQSDIITFHLPLKDETYHLISDKE
                     FEIMKDGVMIVNCARGGIIDEEALVRYLKKGKIKGAALDVFEKELIESSPLFEFENVI
                     VTPHLGASTKEAQVNVACQLATQIVDAIKKKIVKNAVNFPVLDEKTYEKLRGYLSLGE
                     KMGLFLRQMIDGDLKSVEIKYSGEIADYDLTLLRANILVGLLKEEKKVNPINVLLIMK
                     EKGIKLIEKREKTPSEFTNLITVEVKNSKKVSLSGTLVRDEGRIVKIDDFSVEAVPEG
                     FLLICYNEDKPGIMGHIGTILGQKGINIASMTLGRKKKGGPAITVLNLDQEVDREVLL
                     KIKEFPAIKSVRLVRI"
     gene            1684..3081
                     /gene="cysS"
                     /locus_tag="PLF90_03390"
     CDS             1684..3081
                     /gene="cysS"
                     /locus_tag="PLF90_03390"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOK56363.1"
                     /translation="MIKFYNTLTKKKEDFYPISDRFVKMYTCGPTVYDLAHIGNFRAY
                     VFEDLLHRYLEFMDYKVVRVMNITDVDDKTIKGAKKEGISLKEYTDRYIKIFLEDMET
                     LRLKKPDFLPRATEHIKEMVELIKKLLEKGYAYYKDGSVYFSISKFPSYGKLSGIDLS
                     KIKIGARVDTDEYEKEDVRDFVLWKKAKEDEGVWWETEIGKGRPGWHIECSAMSMKYL
                     GETFDIHTGGVDNIFPHHENEIAQSEGATGKKFVNFWLHCAHLLVNGEKMSKSKGNFY
                     TLRDLLNKGYNPVAIRYLLLSTHYRDPLNFTESSLKQAENTVRNYNEFYQGLNICGSE
                     KYNPEIKEEIEKAKKGFIEGLSDDLNISSGLSSIFNFIKKVNVFFSKGEFGKENVKEV
                     KDFIEGIDKVLCIIEKRQETLPEEILNLIKEREKARKNKEFEKADRIRGILKEKGIIL
                     EDTPYGTRWKVSEKP"
     gene            complement(3089..5188)
                     /locus_tag="PLF90_03395"
     CDS             complement(3089..5188)
                     /locus_tag="PLF90_03395"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOK56364.1"
                     /translation="MFIGVQYYRPPFPEKKFWDEDLKKIKETGIDVIQLWACWGWIEN
                     SPGNFNFEDYDYLIERAIKLNLKVVISTIAEIQPFWIEREVPDGIMVDHLGRKVKSIT
                     RKECIVGLTPGGCTDHPKIRDYMIGFLKEIGKRYGKIDEVIGWDCWNETRWNVHSTGY
                     VCYCPETIKKFRDYLKIKYKSIEGLNEAWKRRYILWDDVLPGTTYGKGCTELIEFLNF
                     LSYRASEIMKFRAQSLREVGVKGIITAHCAAPSILSTGWEWEQPLCRGNDWDFLQYLD
                     GYGCSHFPFWGKFDDRIFGIRVESVRSANQGKILWVSELQGGSARDGLSANLPVEAKA
                     QQRWVWNAFGRGAKGLIFWCWKDEVFGHESGGFGISGNDGMAENRLKELKETSIFLKK
                     YDLLLENYKPVTPSVGILFEPLNYFLEWSFEGKVEKSVESLTGYLKVLEDLNIPYEVV
                     ESSHTDILKNLKFLILPFPLVITDKISKIIETFIEKGGIILTEGDMGAFNELAFYRYP
                     GQDRELPYKLGISPVMDRQLIEKEYFNLKIDNKIFKLKSSLWISPLNEKNNKVLAKDE
                     KGRVVAVLSNFGKGTIYAFGTFLGINYGKDKEFDKNFKDFIFYLFKKHNCLPQIKLEK
                     QKEVIYRFGKSGENTLLFVINPAKTKRITFKIPEDLLKNTIIDVRKNREIKLRKKEND
                     EINLELHHYSWDLFIFK"
     gene            5281..5820
                     /gene="pyrR"
                     /locus_tag="PLF90_03400"
     CDS             5281..5820
                     /gene="pyrR"
                     /locus_tag="PLF90_03400"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOK56365.1"
                     /translation="MEKKIMDREEILEILKQMAEIIEKEIGLDNLVIIGIKRRGAIIA
                     DRIKEILKKEIPVGYLDITFYRDDFSKIGAHPVVGTTEILFDIDDKKVILVDDVLYTG
                     RTVRAALDALIDLGRPKFVKLFVLVDRGHRELPVCPDYFGKKVDIGEDEMVEVRLKEI
                     DKKDEVVIVKKWNGKGKTL"
     gene            5793..6716
                     /locus_tag="PLF90_03405"
     CDS             5793..6716
                     /locus_tag="PLF90_03405"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOK56366.1"
                     /translation="MEWKRKDLIGLENLSKEEIEIILEVADSFKDVSTRSVKKVPALR
                     GKTVVNLFFEPSTRTRTSFELAAKRLSADVLSFDVSSSSVSKGETIVDTAKNIEAMKV
                     DCFIVRHSVSGAPEIISKNVSASVINAGDGCHEHPTQALLDLFTVKEKFGKIKGIKIS
                     IIGDILHSRVARSNIWGFKKLGAEVCVCGPPPLIPKDIENIGIKVTYHLEKAIEDADI
                     VYLLRLQRERQKENFLPSLKEYSFLYGLDIEKYEKMKKRTFIMHPGPMNRGIEIKSEI
                     AEKEKSLILEQVTNGIAVRMAVLYLTIGRKV"
     gene            6713..8620
                     /locus_tag="PLF90_03410"
     CDS             6713..8620
                     /locus_tag="PLF90_03410"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOK56367.1"
                     /translation="MSINVYPKISYEEKLNEKQFEIVKNAEGPCLVLAGAGSGKTRVL
                     VYRVCYLIEKGVPPSKILLLTFTNKVAKEMLERVEKILTIYPKGLLGGTFHHVANLLL
                     KIYGKEININPNFTILDEEDSVSIIKEIIEKTGKKDQFPHPSKIKEFISLSINTCESL
                     KETIINFFPEYAFLISEVEKVYKEYQKAKKNLNQLDYDDLLVYWLKLMRNEITGEKIS
                     SRFEYILVDEYHDTNKLQAKILYLLSMKHKNIMVVGDDAQSIYSFRGATIENIIEFPK
                     IYPGTKIFYLDINYRSIPEILNFANNVISHNKIQFPKNLVSIRKNGVKPVVVKCRNMK
                     EEAIFISQRINKLIELRIEPNEIGVLFRSRYQAAELEIELTKLKIPYIIRGGLRFFEQ
                     AHIKDIVAYFRVEENFKDILSWRRILNISEGIGEKGVERIINLILKSENLEDFKNKID
                     EVKLIKKGKNSLEKQIKIIEDLKKQSIAEGIELILNSGYTRYLIKKYKDDVERLEDIK
                     SLKEIASFYSNISDFLSEASLQEYSKGESPNIKNAVVLSTIHQAKGLEWKIVFIIGVC
                     DNHFPHPYSKKDPVALEEERRIFYVGITRAKEDLYITYYSYDFYRYMPVKKSIFIEEV
                     SPDLYEEWDLS"
     gene            8620..9900
                     /locus_tag="PLF90_03415"
     CDS             8620..9900
                     /locus_tag="PLF90_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOK56368.1"
                     /translation="MKLTTLLIFLIAYTGIVLKREKGFYFVFGAVLLLLILRIIVFSE
                     IFKFINYNVLGIFLGTSILSYLFAYSGVPSHIVDSIVEKQYPTGIIFLLICLITGLIS
                     AFVENIATVMIMAPIALEFTKKYKINPVPLFVGMAVSSNLQGCATMVGDSPSIILAME
                     SGLNFNDFFYMPSHKIGAISGKPGIFFFVEFGAILGLFILYLFFKKEKEIHKDFEEKH
                     KINSFFPTFLLILLIFSLAITSFFQEHFSYFPAIICLAYGFIGFLWLFIKKAEKVDLK
                     EIDWESFFLLIGIFILVGSLKKVGIVEDIANFLLKTGKNEFIIYLMIVWISVFISAFV
                     DNIPYTMAMISGIKILCERLNANPYVFLFGLLIGTCVGGNITPIGASCNIVSVGILRK
                     NGYKVKFSDFVKIGLPFSIVSVLGVSLLLWLIYK"
     gene            9921..10790
                     /locus_tag="PLF90_03420"
     CDS             9921..10790
                     /locus_tag="PLF90_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK56369.1"
                     /translation="MNYKIKNMEDKNLIVFTKHFKELDIDKLIEKIKEMGVYGADLCV
                     RPEYPVNPENIENQLPLTVKKFEEEGLTISMITTPTNFNDPENPVVEKVFSACGFSGI
                     KLIKIGYWFMEKDGYWKTVEKIRKKIEKFIKIAEKYDVKFLLHNHSGSTMSLNSSSVM
                     NLIKGFPEEFVGVFLDPGHLSIVGEPIDMAVDIVKEYISAVAIKDLKRERVFVDGKKN
                     WQIRIVPLGEGYVDWEKVLNILFEIKFSGPISIHSEYSELKIDDLIDQTKMDIRYIKR
                     IIEKLKGEKNDSI"
     gene            10777..11835
                     /locus_tag="PLF90_03425"
     CDS             10777..11835
                     /locus_tag="PLF90_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK56370.1"
                     /translation="MTQFEIFKMTVNHRPHNTFLYHAGFTPDLKKRIIEKFNIDEGKI
                     SDFFGFFSPISIQPEEEKREVDFSKYYVDIDKPEGSFINSLGVLEIPAKYYHFTGYIS
                     PLRNAKSLKDIEEFPFPESEKLNPIGMIEKVEKAHKEGKVTVCWVGHMYENSWQIRGY
                     EEFLVDMIENPSWCEYILDRFTDRNIKIAQFAAEAGVDMITTGDDVANQKNLMFSKEM
                     WRKFIKSRWKKVYEKAKSIKPDIQIWYHSDGNIEEIIGELIDIGVTILNPLQPECMDI
                     YKIKREFGKYIVFDGTIGTQSTMPFGTPDEVKRVVRDRKEKIGYDGALILSPTHVLEP
                     DVPIENIEAFVEECRKGY"
     gene            11845..12456
                     /gene="tsaB"
                     /locus_tag="PLF90_03430"
     CDS             11845..12456
                     /gene="tsaB"
                     /locus_tag="PLF90_03430"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOK56371.1"
                     /translation="MKILSIETTTNFGSIALLEYNNLKKEILFESSDIAGEIIEKLNI
                     IYEDFDYVVVSAGPGSWTGIRIGISFAKGLVLADKSKIYVVNVFDSIFYSVKDIKEKF
                     IGIVYSSRDMFFYSEFNGRFNYKNSFKIKKITLKNLLLKIEREDCFLIGPGVINLKEF
                     KNIEKIKTIDYLWYPRASLNGMVAYQKIKRNIPSLLPEPIYGK"
     gene            12446..13519
                     /locus_tag="PLF90_03435"
     CDS             12446..13519
                     /locus_tag="PLF90_03435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK56372.1"
                     /translation="MGNKVKIGYLIGSSEIGGTEKMILTIAKYLDKNIFEIVFFCIKG
                     EGKFTKKLRDENFKVYVFDLKRNPISFLTLLIYLKKEKLDVLQSFLFVGNIIGRIYGK
                     IAGIKFIISSQRSTDPWRKWYHWFLERITERYVDLIISNSYSAREVLIKKGKIPRRKI
                     KVIPNGIEIPVRIERINKDYIVIGAIGNLRKAKGYFNLIKAGKIVIEKFKNVKFYIVG
                     EGELKDEIIREIEKNGLSEYFVLTGYAENIYEYLKIFDIFVLPSLWEGCPVSLLESMG
                     YGIAPVATDVGDVPYIIENGEDGYIVKSGDYKKMAENIIKLIEDKNLREKMGQRAREK
                     IKKNYSFEEMVKAYSSIYLEGKN"
     gene            13567..14109
                     /gene="lepB"
                     /locus_tag="PLF90_03440"
     CDS             13567..14109
                     /gene="lepB"
                     /locus_tag="PLF90_03440"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOK56373.1"
                     /translation="MKKKKDLKKETFNWFKSLFWAFIIALFIRTFFVGNFKIPTTSMV
                     PTLKVGDRLLSNNIIFKIREPKRGEVIIFKPPHDPKRDFVKRLIGLPGEKVMIKEGKI
                     YINGKVIEDPKISCRYYYSIGEYGVEKEIEVSENSYYVLGDNSINSSDSRDWGFVPRK
                     NLIGKALFIYWPPWRMGFIK"
     gene            14106..14678
                     /locus_tag="PLF90_03445"
     CDS             14106..14678
                     /locus_tag="PLF90_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOK56374.1"
                     /translation="MNKKIYYVKEDDLNNLREIAKKFKELLKEKEKSDKKIEELNDKY
                     LRTLAEFDNYRKRIEREKRDILKYGNENLILQLIPFDEIFESVLKQIDRTPSVEVIKK
                     GLELLKKEFEKFLENLGVKKIQAKGEKFNPHFHEATGVIETDDFEDGIIVEEEKSGYL
                     YNDRVIKPSMVKVAKRKSKNNVEDKGTGKN"
     gene            14647..15522
                     /gene="ispE"
                     /locus_tag="PLF90_03450"
     CDS             14647..15522
                     /gene="ispE"
                     /locus_tag="PLF90_03450"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOK56375.1"
                     /translation="MLKIKAPAKINLFLEVGQKNGSLHPLVSLIDIVSLYDFIYLNPS
                     GKTNISFVSKWEIPPDNTVTKLISILKKRFNFEVDIKIEKKIPPGTGLGGGSSDAAYV
                     LIYLNKMFNFGFNIDNMVEIAKKIGSDVPLFLSGKRCLIENYGERFKICNDFKFYYLL
                     LIPEFSVETSDVYNKLDELGEFGNLTYAGEKVRILIEKIEEGDICEMEKYMFNRLEKP
                     CFELNNEIKEVRDEVEKKTGKRFFLSGSGGVLFSIFKEKEEVRKVKNLIFLKNWEKIE
                     VESVMLVKGGKDGNY"
     gene            15509..15898
                     /locus_tag="PLF90_03455"
     CDS             15509..15898
                     /locus_tag="PLF90_03455"
                     /inference="COORDINATES: protein motif:HMM:NF015959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HOK56376.1"
                     /translation="MEITETRISLVERPNSRLRAYASITFDDSFVVRDIRIIEGKRGL
                     FVAMPSKKMQRPCSRCGFKNPISHKFCGSCGASLNPVNQQRLSPSQQHRDLAHPIKTD
                     FREYIQKKVLEEYEKVKKGTGKNYPEQ"
     gene            15945..16015
                     /locus_tag="PLF90_03460"
     tRNA            15945..16015
                     /locus_tag="PLF90_03460"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15977..15979,aa:Gln,seq:ttg)
     gene            16027..16776
                     /locus_tag="PLF90_03465"
     CDS             16027..16776
                     /locus_tag="PLF90_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOK56377.1"
                     /translation="MENKGNLCFVVLAAGMGKRLGGENQKTVTKLLGKPMLKYLLETI
                     KKINPSKIIIVIGFKKEEVFKELEGENVEYVEQKELKGTGDAVLQAEKILNNFKGDII
                     VLNGDVPFISEKTINTLIKIHREENSYCTFLTSIVDNPTGYGRILRNERREVIGIVEE
                     VNASDEEKRIKEINAGVYVFKSDSLFSSLKKIEMNSLKKEYYLTDIIKIYRLENKKIS
                     TYTTPDPDETIGINTLSDLKKAEEYLKKRRK"
     gene            16778..17707
                     /locus_tag="PLF90_03470"
     CDS             16778..17707
                     /locus_tag="PLF90_03470"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOK56378.1"
                     /translation="MDSCLLFSGNSNIELSKKIAKYLNKELSPINIYRFKDGEICVKI
                     EENIRGKDVFIIQSTCPPVNENLMELLITLDALRRASPRRVTAVIPYYGYARQDRKDQ
                     PRVPITAKLVANLLVTAGANRILTMDLHAPQIQGFFDIPVDHLFAAPVIIKYLREKKF
                     KKPVIVSPDVGSVKMARAFAKRLPGDLAIVDKRRESPDKVEMMHVIGNVNGCEVIIVD
                     DLISTGNTMIEAASALLKNGAKVIYGCCTHAVLAGDAVEKFKNSIFEEIIITDTIPHL
                     SLPSKFTVLTVSELLGEAIKSIHLETTVSKLFI"
BASE COUNT         6528 a         2316 c         3044 g         6010 t
ORIGIN      
        1 ttttttttat atccttacca gtctgaccga ttttattgct ggaaactcct ttatttttaa
       61 taatacctct ctatcaactt cttggtcaag atttaaaaca gttattgcag gtccaccttt
      121 tttcttcctt ccaagagtca ttgaagcaat gtttatacct ttttgtccaa gaattgtacc
      181 tatatgaccc ataattcccg gtttgtcctc gttatagcaa ataagtaaaa aaccttcagg
      241 tacagcttca acagaaaaat catcaatttt aacaattctt ccttcatccc ttaccagagt
      301 ccctgataaa gaaacttttt ttgaattttt tacttcaact gtaattaaat ttgtgaattc
      361 agatggagtt ttttctcttt tttcaataag tttaatgcct ttttctttca ttatcaataa
      421 aacatttata ggatttactt ttttctcttc tttcaaaaga ccgacaagta tattagccct
      481 cagtaaagtc aggtcataat ccgctatttc tcctgaatat ttgatttcaa cactttttaa
      541 atctccatca atcatctgtc ttaaaaacaa tcccattttt tctccaagtg aaagataccc
      601 ccttaatttt tcatatgttt tttcgtcaag gacagggaaa ttaacagcat ttttaacaat
      661 tttctttttt attgcatcaa ctatttgagt tgcaagttgg catgcaacat ttacctgagc
      721 ttcctttgta gaggctccga ggtgcggtgt tactataaca ttttcaaatt caaaaagagg
      781 agaactttca atgagttctt tttcaaatac atctaaagcc gcgcctttaa tcttgccctt
      841 ttttagatac cttacaagtg cttcttcgtc tataattcca cctcttgcac agtttactat
      901 cataactcca tctttcataa tttcaaattc tttatcactt attaaatgat atgtctcatc
      961 tttaagtgga agatgaaatg taattatatc actttgttta agtacccctt caaaatttaa
     1021 catagtaaca ccaagatctc ttatcacttc ctctttaaca tacgggtcat atccgacaac
     1081 tttcatacca attccttttg ccatacttgc aactctttta cctattcttc caagaccgat
     1141 tatccccaat gtttttccat atagttcagt tcctaaaaat ttcttttttt cccattttcc
     1201 gttttttaat gtctggtttg cagccggtat atttcttgca agtgaaaaaa taagacctat
     1261 tgtatgttca gcggctgaaa ttgtatttcc ttcaggagca ttcatcacaa ttacaccatt
     1321 taaagtagcc gcctctatat caatattatc aagaccaaca ccggctcttc caattatttt
     1381 aagtttttct cctttttcta ttacctcctt tgttactttt gttccactcc ttactattaa
     1441 tacatcataa ggagttatca tatctattaa ttcttcagag gatactttcc ctgcttcttt
     1501 aacctcaaat ccttcattca caagaatatc ttttccttct ttttcaaatg gctctgttat
     1561 caaaactttt actttttcca ttaatttact cctttaaatt taaattttat tcccattttt
     1621 tgtttttgtc aaaatttatg tatatcctca ttaatgtgat aaaatactta ataaggagaa
     1681 aaaatgatta aattctataa tacacttaca aagaaaaaag aggattttta tccgattagt
     1741 gatagatttg tgaaaatgta tacctgtgga cctactgttt atgaccttgc ccatattggt
     1801 aatttccgtg cttatgtttt tgaggacctg cttcacagat atcttgaatt tatggattat
     1861 aaagttgtaa gagttatgaa tattacagat gttgatgata agacaattaa aggagcaaaa
     1921 aaagaaggta taagtttaaa agaatacacg gatagataca taaagatttt tcttgaagat
     1981 atggagacat tgagattaaa aaaaccagat tttctaccaa gagcaacaga acatattaaa
     2041 gaaatggttg aacttataaa gaaattactt gagaaaggat atgcttatta taaagatggt
     2101 tcagtttatt tcagcatttc aaaattccct tcttatggaa aactttcagg aatagattta
     2161 tctaaaataa aaattggagc aagggtagat acagatgaat atgaaaaaga ggatgtgagg
     2221 gattttgttt tgtggaaaaa agcaaaggaa gatgaaggag tctggtggga aacagaaatt
     2281 ggaaaaggaa gacccggctg gcatatagaa tgttcagcaa tgagcatgaa gtatcttgga
     2341 gagacatttg atattcatac aggaggagtt gataacatat ttccacatca cgaaaatgaa
     2401 attgctcaaa gtgaaggtgc gacaggcaaa aaatttgtaa atttctggct tcactgtgct
     2461 catttacttg taaatggtga aaagatgtca aaatcaaaag ggaattttta tactttgagg
     2521 gacttgctta ataaaggtta taatccagtt gctattagat atcttttact ttcaactcat
     2581 tacagagacc ctttaaactt tacagaaagt tcattaaagc aggcagaaaa tactgtcaga
     2641 aattataatg agttttatca gggtttaaat atatgtggtt ctgagaagta taatccagaa
     2701 ataaaggaag aaatagaaaa agcaaaaaaa gggtttattg aaggactttc tgatgattta
     2761 aatatttcat ctggtttatc aagtatcttc aactttataa aaaaagtaaa tgtctttttc
     2821 tcaaagggag aattcgggaa agagaatgtg aaagaagtta aggattttat tgaaggaatt
     2881 gataaggtat tatgtataat tgaaaaaaga caggagacat tacctgaaga gattttaaat
     2941 ttaattaagg aaagggaaaa agcaagaaaa aataaggagt ttgaaaaagc agatagaata
     3001 aggggaattt tgaaagagaa aggaataata cttgaagata caccttatgg aacaagatgg
     3061 aaagtttctg aaaaacctta aattataatc atttaaaaat aaaaaggtcc catgaataat
     3121 gatgaagttc taaattaatt tcatcatttt ctttttttct taattttatt tctctatttt
     3181 tccttacatc tataatagta ttttttaata aatcttcagg gattttgaaa gttattcttt
     3241 ttgtttttgc tggatttatt acaaaaagga gtgtattttc accgctttta ccaaatctat
     3301 aaataacttc tttctgtttt tcaagtttta tctggggtaa gcaattatgt tttttaaaaa
     3361 gataaaaaat aaaatcttta aagtttttat caaattcctt atcctttcca tagttaatcc
     3421 ctaaaaatgt tccgaaagca taaattgttc ctttgccaaa attacttaaa actgcaacta
     3481 ctctaccttt ctcatctttt gcaagaactt tattgttttt ttcgtttaaa gggcttatcc
     3541 ataatgaaga tttaagttta aaaattttat tgtctatttt taaattaaaa tattcttttt
     3601 caattaactg tctatccata acaggggata ttccaagttt atagggaagt tccctgtcct
     3661 gtccgggata tcggtaaaaa gcaagttcat taaaagcacc catgtctcct tctgttaaaa
     3721 taatccctcc cttttcaata aaagtttcta taatttttga gattttatct gtaattacaa
     3781 gagggaatgg taatatcaaa aattttaaat ttttcagaat atctgtatga ctgctttcaa
     3841 caacttcata aggaatgttt aaatcttcaa gaacttttaa atagccggtt aaactttcaa
     3901 ctgacttttc aactttaccc tcaaaagacc attcaaggaa gtaatttaaa ggttcaaata
     3961 aaattccaac cgaaggagta actggtttat aattttctag tagcaaatca tattttttaa
     4021 gaaaaataga agtttcttta agttctttca atctattttc tgccatacca tcatttccac
     4081 ttataccaaa cccacctgat tcatgaccaa atacctcatc tttccagcac cagaaaatca
     4141 atcctttagc acctcttcca aaagcattcc atacccatct ctgttgtgct tttgcctcaa
     4201 caggtaaatt tgcagaaagt ccatctcttg cagaacctcc ctgaagttca ctaacccata
     4261 aaattttacc ctgattggca cttcttacag attcaaccct tataccaaaa attctatcat
     4321 caaatttacc ccagaaagga aaatgagaac atccatagcc atccagatat tgtaaaaaat
     4381 cccagtcatt ccctctacat aaaggttgtt cccattccca gccagttgaa agaattgaag
     4441 gagcggcaca gtgagcagtt ataatacctt taacaccaac ttctcttaaa ctttgagcac
     4501 gaaatttcat tatttcagat gcacgatagg aaagaaagtt taaaaattca attaattctg
     4561 tacatccctt gccgtaagta gttccaggta aaacatcgtc ccataaaata tatcttcgtt
     4621 tccatgcttc attcagtcct tcaatacttt tatacttaat ctttaaataa tctctaaatt
     4681 ttttaattgt ttctggacaa tagcaaacat atcctgtaga atgaacattc catcttgttt
     4741 cattccagca atcccatcca ataacttcat caattttccc gtatctttta cctatttctt
     4801 ttaaaaaacc tatcatataa tctcttattt tcgggtggtc tgtacatcca cctggagtta
     4861 atcctacaat acactctttt cttgttatag attttacttt tcttccaaga tggtctacca
     4921 taattccatc aggaacttct ctttcaatcc agaaaggttg aatttccgca attgtgctta
     4981 taacaacttt tagatttaac tttattgccc tttcaatcaa gtaatcataa tcttcaaaat
     5041 taaaatttcc gggagaattt tcaatccatc cccagcatgc ccataattga atcacatcta
     5101 tccctgtttc ttttattttt ttaaggtcct catcccagaa ctttttttca ggaaaaggcg
     5161 gtctataata ctgaactcct atgaacattt tttcttctcc ttttaaattt tcaaaatttt
     5221 gcttttatta taacacattt tcaagttttc ctcttaaatt ttttgtggta atataaaagt
     5281 atggaaaaga aaattatgga cagagaggaa atattggaaa ttttgaaaca aatggcagag
     5341 ataattgaaa aagagattgg tttagataac cttgtaatta taggaataaa gagaaggggt
     5401 gcaataattg cagacagaat taaagaaatt ttaaaaaaag aaatacctgt tggatacctt
     5461 gatataacat tttatagaga tgatttcagt aaaataggtg ctcatccagt agttggtaca
     5521 actgaaattc tatttgatat agacgataaa aaagttattt tggttgatga tgttttgtac
     5581 acaggaagga ctgtaagggc agcacttgat gctttaattg accttggaag acctaaattt
     5641 gttaaacttt ttgttcttgt tgatagaggg catagagaat tacctgtatg tcctgattat
     5701 ttcggtaaga aagttgatat tggagaagat gaaatggttg aggtaagatt aaaagaaata
     5761 gataaaaaag atgaggttgt catagtaaaa aaatggaatg gaaaaggaaa gaccttatag
     5821 ggcttgaaaa tttaagtaaa gaagaaatag aaattattct tgaagttgct gactcattta
     5881 aagatgtaag tacccgttct gttaaaaaag ttcctgcttt aagaggtaaa actgttgtta
     5941 atcttttctt tgagccaagt acaaggacaa gaacttcttt tgaacttgct gcaaaaagat
     6001 taagtgctga tgttttaagt tttgatgtaa gttcatcttc tgtttcaaaa ggtgagacaa
     6061 ttgttgatac tgcaaaaaat atagaagcaa tgaaagttga ttgctttatt gtaaggcatt
     6121 ctgtttcagg tgcacctgag attataagta aaaatgtttc tgcttctgta ataaacgcag
     6181 gagatgggtg tcatgaacat ccaactcaag cacttcttga cttatttact gttaaagaaa
     6241 aattcggtaa aatcaaaggc attaaaattt ctattattgg agatatttta catagtagag
     6301 ttgcaaggtc gaatatttgg ggatttaaaa aacttggagc agaagtttgt gtttgtggac
     6361 cacctccttt aataccaaaa gatattgaaa acataggtat aaaagttacc tatcatcttg
     6421 aaaaggcaat agaagatgca gatattgttt atcttttacg acttcaaaga gaaagacaga
     6481 aagaaaattt ccttccatct ttaaaagaat acagttttct ttatggactg gatattgaaa
     6541 aatatgaaaa aatgaagaaa aggacattta taatgcatcc aggtccaatg aacaggggta
     6601 tagaaataaa atcagagatt gcagaaaaag aaaaatctct aattcttgaa caggtgacaa
     6661 atggaattgc tgttagaatg gctgttttat atcttacaat tggaagaaaa gtatgagtat
     6721 taatgtttat cccaaaattt catatgaaga aaaactgaat gagaaacaat ttgagatagt
     6781 gaaaaatgca gaaggtccat gtcttgttct tgctggagca ggaagtggaa aaacaagagt
     6841 tctggtttac agagtgtgtt atcttataga aaaaggggtt cctccatcaa aaattctact
     6901 tttaactttt acaaataaag tagcgaaaga aatgcttgaa agagttgaaa aaattttgac
     6961 gatttatcca aagggtcttt taggagggac ttttcatcat gttgcaaatt tacttttgaa
     7021 aatatacggg aaagaaataa atataaatcc aaattttaca atacttgatg aagaggactc
     7081 agtttcaatt attaaagaaa taatagaaaa aacaggtaaa aaagaccaat tccctcatcc
     7141 ttcaaaaata aaagaattta taagtttgtc aatcaataca tgtgaaagtt taaaagaaac
     7201 aataataaat ttttttcctg aatatgcttt cttaatttca gaggtagaaa aggtttataa
     7261 agagtatcag aaagccaaaa aaaatttaaa tcaacttgat tatgatgatt tacttgttta
     7321 ctggttgaaa cttatgagga atgagattac tggagagaaa atttcttcac gttttgagta
     7381 tatacttgtt gatgaatatc atgatactaa taaacttcaa gcaaaaattc tttatttgct
     7441 ttcaatgaaa cataaaaata ttatggttgt tggcgatgat gcacaaagta tatattcatt
     7501 cagaggtgct acaattgaaa atataataga atttccaaaa atatatccag gaacaaaaat
     7561 cttttatctt gacataaact acagaagtat tccagaaatc ttgaattttg caaataatgt
     7621 aatatcccat aataaaattc agtttcccaa aaatcttgta agtataagaa agaatggagt
     7681 aaaacctgtt gttgttaaat gtagaaatat gaaagaagaa gcaattttta tatctcagag
     7741 aataaataag ttaatagaac ttaggataga accaaatgaa ataggagttt tattccgttc
     7801 aaggtatcag gcagcagaac ttgaaattga acttacaaaa ttaaaaattc catatataat
     7861 aagaggtggt ttaaggttct ttgagcaggc gcatattaag gatattgttg cttattttag
     7921 agttgaagaa aattttaaag atattctttc atggaggaga atattgaaca taagtgaagg
     7981 aataggagaa aaaggagtag aaagaataat aaatttgatt cttaaatcag aaaatctgga
     8041 agattttaaa aataaaatag atgaagtaaa attgataaaa aagggtaaaa atagtttaga
     8101 aaagcagatt aaaattattg aagatttaaa aaaacaatca attgcagaag ggattgaact
     8161 tattttgaat tcaggatata caagatattt aatcaaaaaa tataaagatg atgtagaaag
     8221 gttggaagat ataaaaagtt tgaaagagat tgcatctttt tactcaaaca tttctgattt
     8281 tttatcagaa gcgtctttac aggaatattc aaaaggagaa agtccaaata taaaaaatgc
     8341 ggttgttctt tcaactatac atcaggcaaa aggacttgaa tggaaaattg tttttataat
     8401 tggtgtttgt gataatcatt tcccacatcc ttattctaaa aaagacccgg tggctcttga
     8461 agaagaaagg aggatttttt atgtgggaat aacaagagca aaagaagacc tgtatataac
     8521 ttattattct tatgattttt atagatatat gccagtaaaa aaatccatat ttattgaaga
     8581 agtttcccct gatttatacg aagaatggga tttatcttga tgaaacttac aacacttttg
     8641 atttttttga ttgcctatac aggaattgtt ttgaaaagag aaaaaggatt ttattttgtt
     8701 tttggtgctg ttctcctgct tttgatatta aggataattg tattttctga aatttttaag
     8761 ttcataaatt ataatgttct tggtattttt cttggtacaa gtattctttc atatcttttt
     8821 gcatattcag gagttccctc tcatatagtt gatagcattg ttgaaaaaca gtatccaaca
     8881 ggtattattt ttttgctgat atgtttaatt accggtttaa tttctgcttt tgttgaaaat
     8941 attgctaccg ttatgattat ggctccaatt gctcttgaat ttacaaagaa atataaaata
     9001 aatccagtgc ctctttttgt aggaatggca gtaagttcaa atttacaggg ttgtgctact
     9061 atggttggag atagtcccag tataatcctt gcaatggaat ctggtttaaa ctttaatgat
     9121 tttttctata tgccatctca taaaatcgga gcaatttccg gaaagcccgg aatatttttc
     9181 tttgttgaat tcggagcgat tttgggttta tttattcttt atcttttttt caaaaaagaa
     9241 aaggaaatac ataaggactt tgaagaaaaa cataaaataa attctttttt tccaacattt
     9301 ttactgattt tacttatttt ttctcttgca ataacttctt tttttcaaga acatttttcc
     9361 tatttccctg ctattatctg ccttgcctac ggttttatag gttttttatg gttatttata
     9421 aaaaaagcag aaaaggttga cttaaaagaa attgactggg agagtttctt tttacttatt
     9481 ggaatattta tacttgttgg ttctttaaag aaagttggaa ttgttgaaga tattgcgaat
     9541 tttcttttaa aaacaggcaa aaatgaattt attatttatt taatgattgt ctggataagt
     9601 gtttttattt ctgcttttgt tgataatatt ccttacacaa tggctatgat ttcaggaata
     9661 aaaattttat gtgaaagatt gaatgccaat ccttatgttt ttcttttcgg tcttctaata
     9721 ggaacatgtg ttggtggaaa tataacacca attggagcaa gttgtaatat tgtgagtgtc
     9781 gggattttaa gaaaaaatgg atataaggtt aaatttagtg attttgtcaa aattggactt
     9841 cctttcagta tagtttcagt tttaggagtg agtttacttt tatggctgat ttataaataa
     9901 gataattgac cagtaaatac atgaattata aaataaagaa catggaagat aaaaatttaa
     9961 ttgtttttac aaaacacttt aaggaactgg atattgataa acttattgaa aaaataaaag
    10021 aaatgggtgt ttatggagca gatttatgtg tcagaccaga gtatccagtt aatcctgaaa
    10081 atatagaaaa ccaactgccg cttactgtaa agaaatttga agaagaagga cttacaattt
    10141 ctatgataac aactccaaca aattttaatg accctgaaaa tccggttgtg gagaaagttt
    10201 tttctgcctg tgggttttca ggaataaaac ttataaaaat aggatactgg tttatggaga
    10261 aagatggtta ctggaaaaca gtagaaaaaa taaggaaaaa aatagaaaaa tttataaaga
    10321 ttgcggaaaa atatgatgtg aagtttcttt tacataatca ttctggttca acaatgagtt
    10381 tgaatagttc aagtgttatg aatttaataa aaggatttcc agaggaattt gttggtgttt
    10441 ttttagaccc ggggcattta tctattgttg gagaaccaat agatatggct gtggatatag
    10501 ttaaagaata tataagtgct gttgctatca aggatttaaa aagggaaaga gtttttgtgg
    10561 atggtaagaa aaattggcaa ataagaattg ttcctctggg tgagggatat gttgactggg
    10621 aaaaagtttt aaatatatta tttgaaatta aattttcagg tcctatttca atacatagtg
    10681 aatattcaga acttaaaatt gatgatttga ttgaccagac aaagatggat attagatata
    10741 taaaaaggat aattgaaaaa cttaaaggag aaaaaaatga ctcaatttga aatatttaaa
    10801 atgacagtaa atcacagacc ccataataca tttctatatc atgcaggttt tacacctgat
    10861 ttaaaaaaaa ggataattga aaaatttaat atagatgagg gaaaaatttc tgattttttt
    10921 ggttttttca gtcctataag tatccagcca gaggaagaga aaagagaagt tgatttttca
    10981 aaatattatg tggatattga taaacccgaa ggttccttta taaattcact tggtgtcctt
    11041 gaaattcctg caaaatatta tcactttacc ggctatataa gtccattaag aaatgcaaag
    11101 tcattaaagg atattgaaga attccctttt ccagaaagtg aaaaattaaa tccaataggt
    11161 atgatagaaa aagttgaaaa agcacataag gaagggaaag ttacagtatg ctgggttggt
    11221 catatgtatg aaaattcatg gcaaattcgg ggatatgaag aatttttagt agatatgata
    11281 gaaaacccat catggtgtga atatatactt gacaggttta cagatagaaa tattaaaata
    11341 gcccagtttg cagcagaggc aggagttgac atgataacca caggagatga tgttgcaaat
    11401 caaaaaaatc ttatgttttc aaaagagatg tggaggaaat ttataaaatc aaggtggaaa
    11461 aaggtttatg aaaaggcaaa aagtataaaa ccagatattc aaatctggta tcatagtgat
    11521 ggaaatattg aagagataat aggagaattg attgatatag gcgtaacaat tttaaatccg
    11581 ctgcaaccgg aatgtatgga tatttataaa attaaaagag aatttggtaa atatattgtt
    11641 tttgatggca ccattggaac tcagtcaaca atgccttttg gaacacctga tgaggttaaa
    11701 agagttgtaa gagatagaaa agaaaaaatt ggttatgatg gtgctttaat tttatctcca
    11761 actcatgttc ttgaaccgga tgttcctatt gaaaacattg aagcatttgt agaggaatgt
    11821 aggaaaggct attaaaaatt taaaatgaaa attctttcca ttgaaacaac tacaaatttt
    11881 ggcagtatag cacttcttga atataataat ttaaaaaaag aaattttgtt tgaaagttca
    11941 gatattgctg gtgagattat tgaaaaactt aatattattt atgaagattt tgattatgtt
    12001 gttgtctctg caggacctgg ttcatggaca ggtattcgta ttggtatcag ttttgcaaag
    12061 ggacttgttc ttgcagataa aagtaaaata tatgttgtaa atgtgtttga ctcaattttt
    12121 tattcagtta aagatataaa ggagaaattt ataggtatag tttattcttc aagggatatg
    12181 tttttttact cagaatttaa tggaagattt aattataaaa actcattcaa aataaaaaaa
    12241 attaccttaa aaaatctact tttaaagatt gaaagggaag attgtttttt aatcggtccg
    12301 ggagttataa acttaaaaga atttaaaaat attgaaaaaa taaaaacaat tgattatttg
    12361 tggtatccaa gagcgtcatt gaatggtatg gttgcctatc aaaaaataaa aaggaatatt
    12421 ccatctcttt taccggaacc gatatatggg aaataaagtc aaaattggtt atttaattgg
    12481 tagttctgaa attggtggaa cagaaaaaat gatattaacg attgcaaaat atcttgataa
    12541 aaatattttt gaaattgttt ttttctgtat aaagggagag gggaaattta ctaaaaagtt
    12601 aagggatgaa aattttaaag tttatgtttt tgacttaaaa agaaatccaa tttcctttct
    12661 aactctttta atttatttaa aaaaagaaaa attggatgtc cttcaatctt ttctttttgt
    12721 tggaaatatc attggtagaa tttatggaaa aattgcgggt attaagttta taatttcttc
    12781 ccagagaagt acagacccct ggcggaagtg gtatcactgg tttttagaaa gaataacaga
    12841 aagatatgtt gatttaatca tatcaaattc ttattcagca agagaagttc tgataaaaaa
    12901 agggaaaata ccgagaagaa aaataaaagt tataccaaat ggaattgaaa tacccgtaag
    12961 aatagaaagg attaataaag attatatagt tataggtgca attggtaact tgagaaaggc
    13021 aaaaggatat tttaatttaa tcaaagcggg taagatagtt atagagaaat ttaaaaatgt
    13081 taagttttat attgtaggag aaggcgaatt aaaagatgaa attataaggg agatagagaa
    13141 gaatgggctt tctgaatatt ttgttttaac tggatatgct gaaaacattt atgaatatct
    13201 aaaaatcttt gatatttttg ttcttccttc tctgtgggag ggatgtcctg tcagtttact
    13261 tgaaagtatg ggatacggga tagcccctgt tgctactgat gtcggagatg ttccctatat
    13321 tattgaaaat ggagaagatg gatatattgt aaaaagtggt gattataaaa aaatggctga
    13381 aaacattata aaattgatag aagataaaaa tttaagagaa aaaatgggac agagagcaag
    13441 agaaaaaata aaaaagaatt attcttttga agaaatggtt aaagcatatt catctatata
    13501 tttagaaggg aaaaattgat aaataaaaaa taagatgtta aaatttaaac ttcaaaaaac
    13561 ataaaaatga aaaagaaaaa agatttaaaa aaggaaacat ttaactggtt taaatcgctg
    13621 ttttgggcat ttataattgc tttatttata agaacattct ttgtgggcaa ttttaaaatc
    13681 ccaactactt ctatggttcc aacattgaaa gttggagacc gtcttctttc taataatata
    13741 atttttaaaa tcagagagcc aaagagaggg gaagtcatta tttttaagcc accacatgac
    13801 ccgaaaagag attttgttaa aagattgatt ggacttccag gagaaaaagt tatgataaaa
    13861 gagggtaaaa tatatataaa tggcaaagta attgaagacc caaaaataag ttgcaggtat
    13921 tattatagta ttggagaata tggagttgaa aaagaaatag aagtttctga aaattcatat
    13981 tatgttcttg gagataacag tataaatagt tctgacagta gagattgggg ttttgtcccc
    14041 agaaaaaatc ttataggtaa agcacttttt atttactggc caccttggag aatggggttt
    14101 ataaaatgaa caaaaagata tattatgtga aggaagatga tttaaataat ttaagagaaa
    14161 tcgctaaaaa atttaaagaa cttttgaaag aaaaagaaaa atcagataaa aaaatagaag
    14221 aattgaacga taagtatttg cgtactctgg cagaatttga taattataga aaaagaattg
    14281 aaagagaaaa aagggatata cttaaatatg gaaatgaaaa tcttatttta caactcatac
    14341 catttgatga aatttttgaa agtgttttaa aacagataga caggactcct tcagttgaag
    14401 ttattaaaaa aggacttgaa ctgcttaaaa aggagtttga gaagtttctt gaaaatcttg
    14461 gtgtgaagaa aatacaagca aaaggagaaa aatttaatcc ccattttcat gaagcaacag
    14521 gagttataga gacagatgat tttgaagatg gtataatagt agaagaggaa aagagtggat
    14581 atctttataa tgatagagtt ataaagccat caatggttaa ggttgcaaaa aggaagagta
    14641 agaataatgt tgaagataaa ggcaccggca aaaattaatc tttttcttga ggttgggcag
    14701 aagaatggtt ctcttcatcc tttagtttcc cttatagaca tagtttcctt atacgacttt
    14761 atatatttaa atccatcggg aaaaacaaat ataagttttg tttcaaaatg ggaaatacca
    14821 cctgacaata cagttacaaa attgatttct atcttgaaaa aaagattcaa ttttgaagta
    14881 gacataaaaa ttgaaaaaaa aattcctcct ggaacaggtc ttggtggtgg gagttccgat
    14941 gctgcctacg tgttgattta tttaaacaaa atgtttaatt tcggatttaa tatagataat
    15001 atggttgaaa ttgcaaaaaa aattggttct gatgttcctt tgtttctctc cggtaagaga
    15061 tgtttgatag aaaattatgg agaaagattt aaaatttgta atgatttcaa attttattat
    15121 ttacttttaa tccctgaatt ttctgtagaa acatctgatg tttataataa acttgatgaa
    15181 cttggagaat ttggtaattt gacatatgca ggcgaaaaag ttagaattct tattgaaaag
    15241 atagaagaag gcgatatttg tgaaatggaa aaatatatgt ttaatcgtct tgaaaagcca
    15301 tgttttgaat tgaataatga gataaaggag gtgagagatg aggtagagaa aaaaacagga
    15361 aaaagatttt ttttgagtgg aagtggtggg gttttattca gtatatttaa agagaaggag
    15421 gaggtcagaa aggttaaaaa tttaattttt ttgaaaaatt gggaaaaaat agaagttgaa
    15481 agtgttatgc ttgtaaaagg aggtaaagat ggaaattact gaaaccagaa tttctcttgt
    15541 ggaaagacca aacagtcgtt taagagctta tgcttcaata acttttgatg attcttttgt
    15601 ggtaagagat ataaggatta tagagggtaa aagaggactt tttgttgcta tgcccagcaa
    15661 aaagatgcag agaccctgtt caagatgtgg atttaaaaat cccataagcc acaaattttg
    15721 tggttcatgt ggagcatctt taaatccagt aaatcaacaa cgactttctc cttctcaaca
    15781 gcacagggac cttgctcatc caataaaaac agattttaga gaatatattc aaaaaaaggt
    15841 tcttgaagag tatgaaaaag ttaaaaaagg gacaggaaag aattaccctg aacaataaat
    15901 ttttctatta attaactatt tatttttaaa cttctcagaa ggtttgcccg gtagtgtaat
    15961 ggtaacacac cggcctttgg agccgaggat caaggttcga gtccttgccg ggcaggaaag
    16021 aaaaaaatgg aaaataaagg taatttatgt tttgtagttc ttgcagccgg gatgggaaaa
    16081 cgacttggtg gagaaaatca gaaaacagtt acaaaacttc ttggaaaacc aatgcttaag
    16141 tatcttcttg aaacaataaa aaaaatcaat ccatcaaaaa taataatagt tattggtttt
    16201 aaaaaagaag aagtttttaa ggaacttgaa ggtgaaaatg ttgaatatgt ggaacaaaaa
    16261 gaattgaaag gaacaggtga tgctgtttta caggcagaaa aaatactgaa taattttaaa
    16321 ggagatataa ttgttcttaa tggagatgta ccttttattt ctgaaaaaac aataaatact
    16381 cttattaaaa tacacagaga agaaaatagt tattgtacct ttttaacttc tattgtagat
    16441 aatcccactg gttacggaag gattttaaga aatgaaagaa gagaagttat agggatagtt
    16501 gaggaagtaa atgcaagtga tgaagaaaaa aggattaaag aaataaatgc aggtgtatat
    16561 gtttttaaat cagatagttt attttcaagt ttgaaaaaga tagaaatgaa ttctttgaaa
    16621 aaagaatatt atcttaccga cataataaaa atttatagat tggaaaataa aaaaatctca
    16681 acttatacaa caccagatcc agatgagaca attggaatta ataccttatc tgatttaaaa
    16741 aaggcagagg aatatttgaa aaaaaggagg aaataaaatg gacagttgtt tgctattttc
    16801 aggcaattcc aatatagaat tatcaaaaaa aattgctaaa tacttaaata aagaacttag
    16861 ccctattaat atttatagat ttaaagatgg tgaaatatgt gtaaaaattg aagaaaatat
    16921 aaggggaaag gatgttttta tcattcaatc aacctgtcca ccagttaatg aaaatttgat
    16981 ggaattactt ataactcttg acgctttaag aagggcatct ccaagaaggg taacagcagt
    17041 aatcccttat tatggatatg caagacagga tagaaaagac caaccaagag ttccaataac
    17101 agcaaaactt gtagcaaatc ttcttgttac agcaggagca aacaggattc ttacaatgga
    17161 ccttcatgca ccacagatac agggtttttt tgatataccg gtagaccatc tttttgcagc
    17221 gcctgtaatt ataaaatatc tcagagaaaa aaaatttaaa aaacctgtta tagtttcacc
    17281 tgatgttgga agtgtcaaaa tggcaagagc atttgcaaaa agattaccgg gagatctcgc
    17341 aatagttgat aaaagaagag aatctcctga taaagttgaa atgatgcatg ttataggaaa
    17401 cgttaatgga tgcgaggtaa ttattgtaga tgatttaatt tccacaggaa acacaatgat
    17461 agaagcagca tctgctttgt tgaaaaatgg agcaaaagtt atatatggat gctgtactca
    17521 tgcagttctt gcaggcgatg ctgttgaaaa attcaaaaac tcaatttttg aagaaattat
    17581 tattactgat acaatccctc acctctcttt accttctaaa tttactgttc ttactgtttc
    17641 tgaattactt ggagaagcga ttaaaagcat ccaccttgag acaactgtaa gtaaactttt
    17701 tatctgatta acccattgta ggtttttttc tatggagtgc ttttttactt ctccatctta
    17761 attttctttt ttttcttctc attttttctt actcgcgcat tttttctttg ctgttttttt
    17821 tgctgctttc tttactgttt tttttgcttt tgccaatttc ctcacctccc tttaattttt
    17881 tttaaaattt tacattcc
//
LOCUS       DAOSAT010000026        17815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2199_length_17815_cov_4.877196, whole genome shotgun sequence.
ACCESSION   DAOSAT010000026 DAOSAT010000000
VERSION     DAOSAT010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17815
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2199_length_17815_cov_4.877196"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="PLF90_03475"
     CDS             complement(<1..1419)
                     /locus_tag="PLF90_03475"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOK56379.1"
                     /translation="MVEYIKKRDGSIVEFLPEKIEFAIYRALKSENIDDLNLAIKLTE
                     KVIEKIDKEKIPTVEEVQDIVENVLVENGFYNVAKAYILYREKRKQIRESKKILGVED
                     KLKLSINAINVLRKRYLLRDEKGEIIETPEEMFRRVAKYVASCEKEKSKYEEEFFNIM
                     ANLEFLPNSPTLMNAGTPLGQLSACFVLPVDDSIDGIFTTLKNMALIHKSGGGTGFSF
                     SKLRPKGDIVGSTKGVASGPVSFMEIYDKATEIVKQGGKRRGANMGILSYNHPDIYEF
                     ITSKRKNILTNFNISVSASDEFMEKVIKKEKYSLINPRNGEKVRDVYADEIFDLICQS
                     AWETGDPGIIYIDEINRHNPTPEMGKIEVTNPCGETPLLFYESCNLGSINLTKIIEDG
                     RINWEKLEKIIEIAVRFLDDVIDANNYPIPEIEKATKGNRKIGLGIMGWADLLLELKI
                     PYGSDESFILAEELRKFINEKAF"
     gene            1886..2053
                     /locus_tag="PLF90_03480"
     CDS             1886..2053
                     /locus_tag="PLF90_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56380.1"
                     /translation="MTTEDIIKNIKKTVPISFTMKEAIEKIREWARNRTVPASKVEAK
                     GLTERERKLEF"
     gene            2066..2191
                     /locus_tag="PLF90_03485"
     CDS             2066..2191
                     /locus_tag="PLF90_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56381.1"
                     /translation="MEEDRLKKIEGEKVKSGIIKKKKEKSRFEHLEVGDKKDKRD"
     gene            2337..2822
                     /locus_tag="PLF90_03490"
     CDS             2337..2822
                     /locus_tag="PLF90_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56382.1"
                     /translation="MEKQGIETKKDAYGAVFVENDEDILRKGSSLEIVIDGIRYSSKD
                     PYLPDYIKKALIRIKKENLSPEEIEVKEIKMKKAERKFKIEVEAEKSRYRIIGFSILF
                     TGGILISIFRLLISNKIFKGWIFIFPVIGTLLIIAGLIILSLGLSPDEIERFFGRGKW
                     W"
     gene            2956..3147
                     /locus_tag="PLF90_03495"
     CDS             2956..3147
                     /locus_tag="PLF90_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56383.1"
                     /translation="MEEIKYLTIENEDEIMEKVDILVENQEGVKIGFHKQKDGVYKII
                     PGKVSSEKRRRNRKNLLDR"
     gene            complement(3525..5606)
                     /locus_tag="PLF90_03500"
     CDS             complement(3525..5606)
                     /locus_tag="PLF90_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56384.1"
                     /translation="MRKIISVIILLFYTQQVLSFSFFQRYKPEELYPISSEYFKQNFK
                     KVKREKIKIPEGVLILPLLDKESKLNSLGTLISILATYKYMYLPEKKLNILMPDLESI
                     FGNNFYKDGKIINFRPKQIKRLMEEMRVNTYVTGFFIHNNKNFHVELRFEGPKGDFNI
                     GREGKIEEIIYIPSWIAENVYKYLGVRISKEKREFMNKLDFYDWRDMVEVASVENLLF
                     KNIYCLTPADDKKLLNTYRKFYLKNPDSIFLIIRYANALWEMHNDKEALDVLERVMKK
                     YPDCERILLCYCHILDSKAYEPCGYDRVIVPILMKLLKTEYLNKNVYSTLIGTYKPYN
                     LWEECETLYNFWVENDPNYYLPYQERGKFYIEYAWYARGGGWAYTVTKEAWRKYFQRL
                     NLAEKDLLKAVSLNPEAYNAYARLVQIGYEMDRSYEYIDYFYKRAIEIQPENVSVYFQ
                     KFFKLQPKWGGSLDEMYDFIDELIEKNCKDFIITNLFEIFLNSKFDDKNYYYQKDEIW
                     ERIKELFEKLIKKNPDTIYTRYVVLKMAFLRKDYQYAKNILNKLGSYEDNYIFADFEE
                     FIETKKKIIMTLNNVDELGAYKVLIKEYRDWIKKEPDKIFNYIILAYLLNKIKDYKNA
                     EFMISKVEEISMHIPDAYYVKGLIYLDKGEKEKAKECFWRACLTIEHQFSGGVPAIMR
                     FEKYLTWYNKL"
     gene            complement(5644..6777)
                     /locus_tag="PLF90_03505"
     CDS             complement(5644..6777)
                     /locus_tag="PLF90_03505"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOK56385.1"
                     /translation="MKIIVAPNSFKGCLTSIEVTEIIEKEIKKILPSSEIIKFPLADG
                     GDGTLEVLKSLIGGKYIEIEATDPLRRKIKTKYIKKNNVAYIEMAKISGLALLKENEK
                     NPLKTTTFGLGEIIIDAIDKGCKKIYIGVGGSATNDGGTGALTAIGFRFINKNGKIIY
                     PGKGEDLLGINKIEYPENYEKLKKIEFTILSDVKNPLYGKNGAAYVYAFQKGANESMV
                     EILDNGLRNYAKVIKKYTGIEINKVEGSGAAGGIVAGFVSFLKSKVISGIEFILKIGN
                     FEEKIKKADIIITGEGKIDTQSFFGKAVGVILKYSEKYKITVIVLAGIVDEKIYRFLK
                     SPYISIFSIVPGPVPLKDSIENSKKYLEIRTREILKLLKINLS"
     gene            6919..7179
                     /locus_tag="PLF90_03510"
     CDS             6919..7179
                     /locus_tag="PLF90_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOK56386.1"
                     /translation="MYWVYVLKNKEGRIYIGQTEDIEKRLEYHNRGWSRYTKGKGEWE
                     IVLKECYNSRKEAIKREKELKTGKGREYIKEKIKNRGVAQSG"
     gene            7156..7231
                     /locus_tag="PLF90_03515"
     tRNA            7156..7231
                     /locus_tag="PLF90_03515"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7192..7194,aa:Pro,seq:tgg)
     assembly_gap    7257..7356
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7615..8697
                     /locus_tag="PLF90_03520"
     CDS             7615..8697
                     /locus_tag="PLF90_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK56387.1"
                     /translation="MKRVFKCEEVDRMPIRIWGVSPVFPTENFKPLCELVEKYDLETI
                     EIWEPKKDEIPPLPYRIETEEKIIEKEGMKEIITKIKAPKGDLIQVYKHKLGGSPGRV
                     IKNFIEDIKDAEKFLSLPDIRELPKIDSYFEFEKKVGERGLILIGIDEAMYAVHRFMG
                     SEVFSFFLYDHREILHNMIEKKFKEIENYVKYILSNKIGDGFGYVGPELCIPPLASVN
                     DFNEFVFDYDKKIIDLIHDEKKFVWVHCHGDMAPVLERFIDMGVDCLNPVEPPPVSKM
                     TLKEMKEISKGKMCLEGGVEDGAFDTLKPDEMKKLVEKIIEEGKPGGGFILCPTSSPN
                     TWPKLLPRHIENYKIFVETGVKLRNY"
     gene            complement(8694..9800)
                     /locus_tag="PLF90_03525"
     CDS             complement(8694..9800)
                     /locus_tag="PLF90_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOK56388.1"
                     /translation="MEKEFSFQEFVELSKERNASDIHLQVGSKPILRIKGDILRIEEY
                     KKLSEDDIGKIIYEFLQEKEKKILEETGNLDTSMSFPNIGRFRVNIFKQRGTFALVAR
                     RITSTIPDFEILNLPESTKKVCDYINGLVLVTGPTGSGKSSTLAAIINKINITRTCHI
                     LCIEDPIEYLYKNDKAIITQREIGIDVRSFKEALKYAVRQDPDVILIGEIRDEETVEF
                     AIHSAETGHLVFGTLHSQNAPLTIGRILNFFPEVRQPQIRRDLSLHLRCVISQMLLPS
                     IKEGVQRVPAVEIMFVNSLISRLILEGQDSKIPKAIKAGKNEGMQDFEDSLLDLYNRG
                     FIDKETALKYAENPQSLDMKMKGIFLAEEGGIVI"
     gene            9831..10313
                     /locus_tag="PLF90_03530"
     CDS             9831..10313
                     /locus_tag="PLF90_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOK56389.1"
                     /translation="MKIIIVYFSLTGNTRKLSVKIYEILKSKNLDVSLFEIESKERCS
                     FLKNCFNAIFEKLIKIDKVPHIENYDLLFIGTPIWAGKITPHIRSFLEDIDLKNKKVF
                     LFTTYGSGFLKNKAMKEFIELVETEGGKIVDMFEVRGEKIEKYMDNLKEKIEKCLKEL
                     "
     gene            10295..10681
                     /locus_tag="PLF90_03535"
     CDS             10295..10681
                     /locus_tag="PLF90_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOK56390.1"
                     /translation="MFERIITEKSPLPVGPYSQGIKWKNFIFISGQIPVDPKTNTVVN
                     GDITSQTKQVIENIKNILTEAGADLENVVKTTVFLKNINDFEKMNYVYAQYFKNKPAR
                     TTVEVSNLPKNVLIEIEVIAIIEEGK"
     gene            10678..11079
                     /locus_tag="PLF90_03540"
     CDS             10678..11079
                     /locus_tag="PLF90_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56391.1"
                     /translation="MRQKLFFIFTLGFISGVICSTKISRMQRVEFVNWPQMKFKNIRI
                     LIAGTEKLSNQGFAGASLKEMENTIIIFPNIPDGVVFTNVDQGFGAVKENIKIYYLDK
                     NFKILKYDIMEKEKGISIPTENTFIAVEGLP"
     gene            11117..11818
                     /locus_tag="PLF90_03545"
     CDS             11117..11818
                     /locus_tag="PLF90_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HOK56392.1"
                     /translation="MKKLLILLILLIFNTGFSETKDKYIRRFKVAEDLLEYMLKQPDV
                     SIPVNLIKECYGIIFIRQFKGGFLLAGKGGEGIIISRDREKRTWSAPGFIASIQVSFG
                     WQIGAQMTDLILLIMNEDGLRMLTTSKVKIGADIGVAAGPVGREFEGKVSPGAGILAY
                     GRSKGVFGGLSLEGGAIFSDDKANEEFYGKKGLKLKEIIFDKKVSIPKEAEKLIKLLK
                     DYEEAELVIEKKEKK"
     gene            11821..13179
                     /locus_tag="PLF90_03550"
     CDS             11821..13179
                     /locus_tag="PLF90_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56393.1"
                     /translation="MKKYIFLLIILTGCILNIPSIEKEKINIFKDRKDAIFSPSGDIN
                     NSEFAISPNGEFYAKEIEPYNHGRIGIFLKNGELIKEINVFDENEAENDLKVLAWHPD
                     NFVIGVVYHKDGGSEIKMYEIFTGKLLRKIILGNFYHYFVFDENGEKIYLSVDGEKID
                     EVNLRNTDFVFNSGINLPWLNYGWDIGRNPWEDKHGGFSSNKEVLNEKFKFFKEIGVN
                     VVRVFLFCDLRSGVIFDNNRLTFDKYVYKDFNTLIEITENIGIKILPVLFDFTIADGV
                     EEENGIKVGEHPEIFYDIRVQKELLNLFEGFFRKIETKKVIYGWDIINEPEHLKVDSS
                     KVDNFISAFIKLIRTIKRNEKITVGALSRVYLSKYKKFNLDFYQFHYYDSFENNSSLD
                     YHFYNLLIEKPVIIGELEPTEIPDKLTKIWERGYKGVLIWANEEFLKDGWRLYKAWID
                     VH"
     gene            13202..13630
                     /gene="nusB"
                     /locus_tag="PLF90_03555"
     CDS             13202..13630
                     /gene="nusB"
                     /locus_tag="PLF90_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOK56394.1"
                     /translation="MLRRKAREYALKILYMIDMREDTNSTFLENFEENEKVKKFADEI
                     IKGVIENMKEIDEIISKVSLNWDIKRMSYIDRNILRIATYEIVFREDIPSIVSINEAI
                     EISKKYGDEDSPKFINGILHKIKEIYENNKKEKKGDENEI"
     gene            13620..14195
                     /locus_tag="PLF90_03560"
     CDS             13620..14195
                     /locus_tag="PLF90_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOK56395.1"
                     /translation="MKFKIFLSFLFLIFLITNLSGKEKKVLMVIAFENFRDEELKVPK
                     EILEKNGIIVDIASVKKGIAKGMLGTKIDINLTLEEIKENDYDGIIFVGGIGSQSLFT
                     NTHAIKLAQNFYKSKKVVGAICLAPGILAKAGILNGKKATCFYTASEILKKNGAIYTG
                     NLVEIDENIVTGNGPEAAEKFGNEILRLIKK"
     gene            14192..15154
                     /locus_tag="PLF90_03565"
     CDS             14192..15154
                     /locus_tag="PLF90_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOK56396.1"
                     /translation="MKKNVIFTIVRILISVFFIYLILQKVNFKKISEIIKNANYLPLI
                     FAFITIFSINFLLALRFKYILEIYFKKKLSYFLIWKLTMIGLFFNNFLPTSAGGDIVK
                     IFYIVKEQEKKFLSGISVLIDRYIGALSIMIMGTISVFFYKGIEKKFQYIIFVFLFFL
                     IFAFFFFSKRKIASLFYSPIKKFIPEFLNRKLLNLYSSIYFYFTENSKNLKIAILISF
                     ILQILSIFSQYLIGVSIIKSNLNVLLFFVFIPLIWTSTLIPSLGGLGVREFTYIFLFS
                     DFIGEQNAYALSILVLTTVVLNSLIGGFIFLTFETKRKLTEKNY"
     gene            complement(15164..16123)
                     /locus_tag="PLF90_03570"
     CDS             complement(15164..16123)
                     /locus_tag="PLF90_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HOK56397.1"
                     /translation="MPSLIYTISLNSSIDYSFYLSNIIDDDVNRIEKTRIDPGGKGMN
                     ISRMLRKLGEKPITITFLSKNNGRLYKKLLKKEKIKIVPIMIKGNLRNIYNFISEKKV
                     LRFNEKGPLITNKEINKFWEILLNLNLKKGDFVVMSGSIPPGIKNNIYAEIIKRVKKF
                     EVLTIVDADGDVLRESIKEKPFLIKPNLQEIERCFDTKIKNFETLKNICISLINSDIS
                     IVLITLGKDGAILFTKDKLFYSKPPEVDFKSSIGCGDAFLSGFIYKLKRKKTYEECLK
                     FAVACGTAKVEEEGTKMPSLSRIKKVLKYVRIYENPDFNISFQ"
     gene            complement(16126..16305)
                     /gene="rpsU"
                     /locus_tag="PLF90_03575"
     CDS             complement(16126..16305)
                     /gene="rpsU"
                     /locus_tag="PLF90_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOK56398.1"
                     /translation="MVNNTNFEKIFKQFKREVEREGIIKEIKAREFYEKPSEVRKRKK
                     MRKYRPHLNTIIKSK"
     gene            complement(16347..16484)
                     /locus_tag="PLF90_03580"
     CDS             complement(16347..16484)
                     /locus_tag="PLF90_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56399.1"
                     /translation="MKTRTKPKITVRCRKCFMVDSYIIEEGKEIKCNHCGAKIYKIDS
                     I"
     gene            complement(16499..17317)
                     /locus_tag="PLF90_03585"
     CDS             complement(16499..17317)
                     /locus_tag="PLF90_03585"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOK56400.1"
                     /translation="MKEFEKAKEAAEFIRSKIKVKPDIGIILGTGLGALAEKIEESII
                     IPYKEIPYFPMSTVETHLGNLVIGKIGNKNVVAMQGRFHLYEGYTPYQVVFPIRVMKL
                     LGINILIESNAAGGLNPLYKKGDIVIIVDHINLTGQNPLVGENDERFGPRFPDMFSPY
                     SPDLIEIAEKVGIEEKIPLKKGVLVGLLGPNFETKAEYRFLRSIGADMVCMSTVIEVI
                     AAVHLGLKILGLSVITDMCLPDALKPVSFEEIVEVANSAEPKLTKIVEKVIINL"
     gene            17563..>17815
                     /locus_tag="PLF90_03590"
     CDS             17563..>17815
                     /locus_tag="PLF90_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOK56401.1"
                     /translation="MKKFNYSIGIDVSKDSFVVSIKGNDFIKSNLSFSMDKSGFENFA
                     SQTIQFKNSSVCACEPTSIYHINLVNFLKEKGYNVCMVNP"
BASE COUNT         6268 a         2410 c         2670 g         6367 t
ORIGIN      
        1 aaatgctttt tcatttataa atttcctcaa ttcttctgca agaataaaac tttcatcgct
       61 tccatagggt attttcagtt caagtaataa atcagcccat cccataattc caagcccaat
      121 ttttctattc ccttttgttg ctttttcaat ttcagggata gggtaattat tcgcatcaat
      181 cacatcatca agaaatctga cagcaatttc tataattttt tcaagttttt cccaatttat
      241 tctcccatcc tctattattt ttgtcagatt tattgacccg agattacatg attcgtaaaa
      301 aagcagagga gtttcaccac agggatttgt tacttctatt tttcccattt caggagttgg
      361 attgtgtctg ttaatttcat caatataaat aattccagga tctcctgttt cccatgcact
      421 ctgacatatc aagtcaaaaa tctcatcagc ataaacatcc ctgacctttt ctccatttct
      481 tgggtttata agtgagtatt tttctttctt tataaccttt tccataaatt catcacttgc
      541 acttacagag atgttaaaat ttgtaaggat attttttctt tttgaagtta taaattcata
      601 aatatcgggg tggttataac tcaaaattcc catatttgca cctcttcttt ttccaccctg
      661 tttaacaatt tccgttgctt tatcatatat ttccataaaa gatacaggac ctgaagcaac
      721 tccttttgta gaaccaacaa tatctccttt gggtcttaat tttgaaaatg aaaatcctgt
      781 tccaccacca cttttatgaa ttaaagccat attttttaat gttgtgaata tcccatcaat
      841 agaatcatca acaggcagaa caaaacatgc agaaagttga ccgagaggtg ttcctgcatt
      901 cattaatgtt ggagaatttg gtaaaaattc aagatttgcc ataatattga aaaattcttc
      961 ttcatattta cttttttctt tttcacatga tgctacatat ttcgcgactc ttctgaacat
     1021 ttcctcagga gtttcaataa tttctccttt ttcatccctt aataaatatc tctttcttaa
     1081 aacatttatt gcattaatag aaagtttgag tttatcctcg actccaagaa tttttttact
     1141 ttctcttatc tgttttcttt tttctctgta aagaatatat gcctttgcaa cattataaaa
     1201 accgttttca acaagaacat tctctacaat atcctgaact tcctcaactg ttgggatttt
     1261 ttctttatct attttttcta tgactttctc cgttagtttt attgcaagat ttaaatcgtc
     1321 aatattttct gattttaatg ctctatatat cgcaaattct attttttctg gcaggaactc
     1381 tacaattgaa ccatctcttt ttttgatata ttctaccatt gattataaaa tacctcttta
     1441 aattcttatt gtcaataaga tttctctttc aggttaagac atagtatcca gggatataat
     1501 atatggggaa gaaatatctg gttaagaaga catatagaag tatttgttcc aaagagtaag
     1561 aaagaagcaa aaaagttgat taaaaagatt tttgaaaaag gagaatggcg cttgtcaaga
     1621 tttttttctc ggatatcacc tcctttccat accgcaagtt aaacaattgt cttatttcat
     1681 attcattccc tttatcttac tgttaattct ctaaatatat ccctctttct gctaacataa
     1741 actcattaaa taattcctca aaaagtttac acaacccaac tttctctttg atttacttgc
     1801 aagagacagt gaaaattttt cagaagcaga aattgaacaa gcaataatat ctgcactttt
     1861 tgatgtattt gttgaaaaca gggatttgac tacagaagat attattaaaa acattaaaaa
     1921 aacagttcca atctcattta ctatgaaaga ggcaattgaa aaaataagag aatgggcacg
     1981 taatagaact gttcctgcga gtaaagttga ggcaaagggt ttaacggaaa gagaaaggaa
     2041 acttgaattc taaaaggaat tatttatgga agaggataga ttaaaaaaaa tagaaggaga
     2101 aaaggtaaaa tcaggaatta taaaaaagaa aaaagagaaa agcagatttg aacatcttga
     2161 agtaggagat aaaaaagata aaagggatta agaagtaaaa tatttcccct gtccaaaatg
     2221 taaaagaatt aatcaaaagg gtaatttata ctgtatttat tgtggatatg ttttccctga
     2281 agttgctgaa aaaacagatt cagagttaaa tccttatgaa ataaaatgtc cttcctgtgg
     2341 aaaaacaggg aatagaaacc aaaaaagatg catatggtgc ggtttttgta gaaaatgatg
     2401 aggatatttt gagaaaagga agttctctcg aaattgttat agacggtatc agatattcat
     2461 caaaagaccc gtatctgcct gattatatta aaaaagcact tataagaatt aaaaaagaaa
     2521 atttaagtcc tgaggagata gaagttaaag aaataaaaat gaaaaaggcg gaaagaaaat
     2581 ttaaaattga agtagaggct gaaaagagca gatatagaat tattggtttt tccattctct
     2641 ttacaggagg aattttaatt tcaatttttc gtttactaat ttcaaataag atttttaaag
     2701 gatggatatt tatatttcct gtaattggaa cacttctaat tattgcaggt ttaataatat
     2761 tgtctctagg tttaagtccc gatgaaatag aaaggttttt tggaagaggt aaatggtggt
     2821 aaaaaggagg aaaatatgag tgcttgtgga ggaattgaac ttataatctt aagtactact
     2881 cttttactga agacattgta aacattaagt gaattaaaat tacaaacttc tattaaaaaa
     2941 gcacttgata aagaaatgga agaaattaag tacttaacaa ttgaaaacga agatgaaata
     3001 atggaaaaag tggatattct tgttgaaaat caagagggag taaaaatagg atttcataaa
     3061 cagaaagacg gggtatacaa aataatcccg ggtaaagtct cttcagaaaa aagaagaaga
     3121 aacagaaaaa acttattgga cagataaaac agagatatgc ctataatctt gtaaaagaag
     3181 aactgacaaa aagaggttat acaatagttg aagagaaaaa tctgaaaaaa atttgattaa
     3241 aatagtagca aggagatgga gataaaatgg cagaaaaaga agaaattgaa atttttgtga
     3301 atgaagaagg agagatagaa tttttaattt aagggataaa aggaccagat tgtcataaag
     3361 ttgcaaaaga attggggagt attgttggaa agataaaaga aataaagaat acatctgagt
     3421 attatgaaaa aacatccact aaaacaaaaa ttgagaaaaa aatgatatag gtgacttggt
     3481 agaatttact cttttttgaa aaaactaaga aaagaactta aagttcatag tttattatac
     3541 catgttaaat atttttcaaa tctcattatg gcaggtactc ctcccgaaaa ttgatgttca
     3601 attgtcaaac atgccctcca gaaacattcc tttgcttttt ctttttctcc tttatcaaga
     3661 taaatcaatc ctttaacata atatgcatca ggaatatgca tactgatttc ttctactttt
     3721 gatatcataa attctgcatt tttataatct ttaattttat tcaaaagata ggcaagaata
     3781 atataattaa aaattttatc aggttctttt tttatccagt ctctgtattc cttaattaaa
     3841 accttataag ctcccaattc atctacatta ttcaaggtca taattatctt tttcttcgtt
     3901 tcaataaatt cttcaaaatc agcaaagatg taattatctt cataagaacc gagtttatta
     3961 agtatgtttt tagcatattg gtaatccttt cttaaaaaag ccattttcaa aactacatat
     4021 cttgtataaa tcgtatccgg attttttttg ataagttttt caaataactc ttttattctt
     4081 tcccatattt cgtctttctg ataataataa tttttgtcat caaattttga attgagaaaa
     4141 atttcaaata aatttgtgat aataaaatct ttacaatttt tttctataag ttcatcaata
     4201 aaatcataca tctcatctaa tgaaccaccc cattttggct gtaacttaaa gaatttttga
     4261 aaatagacag atacattttc aggctgaatt tctattgctc ttttataaaa ataatctata
     4321 tattcataac ttctatccat ttcataacca atttgaacaa gtctggcata cgcattatat
     4381 gcttcaggat ttaatgaaac agcttttagt aaatcttttt ctgcaagatt taatctttga
     4441 aaatatttcc tccatgcttc ttttgtaacc gtgtaagccc atccaccacc tctcgcatac
     4501 catgcatact caatataaaa ctttcccctt tcctgatatg gtaaataata attcgggtcg
     4561 ttttctaccc agaaattata taaagtttca cattcttccc ataggttata aggtttatat
     4621 gttccaatta aggtagaata aacattttta tttaaatatt ctgtttttaa gagtttcata
     4681 agaatgggga caataactct atcatatcca catggttcat aagcttttga atcaaggata
     4741 tgacaataac ataaaagtat tctttcgcaa tcaggatatt ttttcattac tctttcaaga
     4801 acatccaagg cctctttgtc attatgcatt tcccacaaag cattagcata tcttattatg
     4861 agaaatattg aatcaggatt ttttagataa aattttctat atgtatttaa aagtttttta
     4921 tcatcagcag gagtcaggca gtaaatattt ttaaaaagga gattttcaac acttgcaact
     4981 tcaaccatat ccctccaatc gtaaaaatct aacttattca taaattccct tttttctttt
     5041 gaaattctaa ccccaagata tttataaaca ttttctgcaa tccatgatgg aatgtagata
     5101 atttcttcta tctttccttc tctaccaata ttaaaatctc ctttgggacc ttcaaatctt
     5161 aactcaacat gaaaattttt attattatgt atgaagaagc cggtaacata agtattaact
     5221 ctcatttctt ccattaatct ctttatctgt tttgggcgaa aattaataat ctttccatct
     5281 ttgtaaaaat tgtttccaaa aatagattct aaatctggca tcaaaatatt caattttttt
     5341 tctgggagat acatatactt atatgttgcc agaatagata tcagggtacc caaagaattt
     5401 aatttacttt ctttatcaag aagaggtaaa atcaataccc cttcaggaat tttaattttt
     5461 tctcttttta cctttttaaa attctgttta aaatattcag aagatatagg gtaaagttct
     5521 tctggtttat aacgttgaaa aaaagaaaat gaaagaactt gttgagtata aaaaagtaaa
     5581 ataataactg aaattatttt tctcatcttc ttaaaaaaat tataaaaaat taaacaaaat
     5641 ttttcaagag agattaattt tgagcaattt taaaatctct cttgtcctta tttccaaata
     5701 tttttttgaa ttttcaattg aatctttaag aggaacagga ccaggaacaa tactgaaaat
     5761 tgagatatat ggacttttta aaaatctgta aattttttca tcaacaattc cagcaagaac
     5821 tattacagta attttatatt tttctgaata cttcaaaata actcctactg cttttccaaa
     5881 aaaagattga gtatcaattt taccctctcc tgttattata atgtctgctt tttttatttt
     5941 ttcctcaaaa tttcctattt ttaaaataaa ctctatacct gaaataactt ttgacttgag
     6001 aaaagaaaca aaaccagcaa cgattccacc tgcagcacca gaaccttcta ctttatttat
     6061 ttctattcct gtatatttct ttataacttt tgcataattt cttaatccat tgtcaagaat
     6121 ttcaaccata ctttcatttg cacctttctg gaaagcgtat acataggcag caccattttt
     6181 accatacagg ggatttttta catctgaaag gattgtaaat tcaatttttt ttaatttttc
     6241 ataattttca gggtactcaa ttttatttat tcctaaaagg tcttctcctt ttccaggata
     6301 aataattttt ccattcttat taataaatct aaaaccaata gcagttaaag cacctgtccc
     6361 accatcgttt gtagcgcttc ctccaacccc tatatagatt tttttacatc ctttatctat
     6421 tgcatcaatt ataatttctc caagtccaaa agttgttgtt tttaaaggat ttttttcatt
     6481 ttctttaaga agtgcaaggc cagaaatttt agccatctct atataagcaa cattgttttt
     6541 tttaatatat tttgttttta tttttcttct aagagggtca gttgcttcaa tttcaatata
     6601 tttaccgccg attaaacttt ttaaaacttc aagagttcca tctccaccat cagcaagagg
     6661 gaattttatg atttctgatg aaggtaaaat ttttttaatc tctttttcaa ttatttctgt
     6721 tacttcaatt gatgttaaac aacctttaaa agaattagga gcaactatta ttttcataca
     6781 aaaattataa ttgatttttt cagattttga aagtataatt atttgtagtt tatttcgggg
     6841 cgtagcgcag tccggttaag cgcgtccgcc tgaggcggaa ggtcctcggt tcaaatccga
     6901 gaaatattaa gtaaaaatat gtactgggtg tatgtattaa aaaataaaga aggtagaata
     6961 tatataggtc agacagaaga tatagaaaag aggttagaat atcataatag aggatggagt
     7021 aggtatacaa aggggaaagg ggagtgggaa atagttttaa aagagtgtta taatagtaga
     7081 aaggaagcga taaaaagaga gaaggaatta aagacaggaa aaggaaggga gtatattaaa
     7141 gagaaaataa agaatcgggg cgtagcgcag tccggttaag cgcgcttggt ttgggaccaa
     7201 gaagtcctcg gttcaaatcc gagcgccccg acttattcag ataaatttca gataaannnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntagt aggatagaag aggaatcgta
     7381 gtccggttaa gcgcgtccgc ctgaggcgga aagtcctcgg ttcaaatccg agcgccccga
     7441 cttattcaga taaatttcag ataaaatagt taaaaaaatt gaaaattagt aggatagaag
     7501 aggaatcgta gtccggttaa gcgcgtccgc ctgaggcgga aggtcctcgg ttcaaatccg
     7561 agcgccccga ctttaattag ataatgaatt tttaatatga ctcacagaga gagattgaaa
     7621 agagttttta aatgtgaaga agttgataga atgcctatca ggatttgggg agtaagtcct
     7681 gtttttccta ctgaaaattt taaaccatta tgtgaacttg tggaaaaata tgaccttgaa
     7741 acaattgaaa tatgggaacc aaaaaaagat gaaatcccac ctttacccta tcggatagaa
     7801 acagaagaaa aaattataga aaaggaagga atgaaggaaa taataacaaa gattaaagca
     7861 cccaaaggag atttaattca ggtttataag cataaacttg gcggatctcc gggaagggtt
     7921 atcaaaaatt ttattgaaga tataaaggat gcagagaagt ttctttcatt gcctgatatt
     7981 agggaattgc caaaaattga ttcttatttt gaatttgaga aaaaggttgg agaaagaggg
     8041 ctgatactta taggaattga tgaggcaatg tatgcagttc acagatttat gggttcagag
     8101 gttttcagtt tttttcttta cgaccatagg gaaatccttc ataatatgat agagaaaaaa
     8161 tttaaagaaa tagaaaatta tgttaaatat attctatcaa ataaaatagg ggatggattt
     8221 ggttatgtgg gtcctgaatt atgtatacct cctcttgcat ctgtaaatga ctttaatgaa
     8281 tttgtttttg attacgataa aaagataata gatttaattc atgatgaaaa gaaatttgta
     8341 tgggttcact gtcatggaga tatggcacct gttttagaaa gatttataga tatgggagtg
     8401 gattgcttaa atccggtaga acctcctcct gtatctaaaa tgacattaaa ggagatgaaa
     8461 gaaataagta aaggcaaaat gtgtcttgaa ggtggagttg aagatggtgc ttttgatact
     8521 ttaaaaccgg atgagatgaa aaaacttgtt gaaaaaatta tagaagaggg aaaacctggg
     8581 ggtggattta ttttatgtcc tacttcttct cctaacacct ggcctaaatt attacccaga
     8641 catattgaaa attataaaat ttttgttgaa acaggtgtga aattaaggaa ttattaaatg
     8701 acaattccac cttcttctgc aaggaaaatt cctttcattt tcatatcaag tgattgagga
     8761 ttttctgcat attttaaagc agtttctttg tcaataaaac ctctattgta aaggtctaaa
     8821 agagaatctt caaaatcctg cattccttca ttttttcctg cttttattgc ttttggaatt
     8881 ttactatcct gtccttcaag aatcaatctt gaaatcaaag aattgacaaa cattatttca
     8941 actgcaggga ctctttgaac accttctttt attgatggta aaagcatttg agaaattaca
     9001 catcttaaat gtaaactgag atctcttctt atctgtggtt gtcttacttc aggaaagaaa
     9061 ttcaaaattc ttccgatagt aagaggtgca ttttgactgt gtaaagttcc aaagacaaga
     9121 tgacctgttt ctgctgaatg tatagcaaat tctactgttt cttcatcacg tatttctcca
     9181 ataagaatga catcagggtc ctgtctcaca gcatatttta atgcttcttt aaaacttctc
     9241 acatctatac ctatttctct ttgtgttatt attgctttat cgtttttata aagatattca
     9301 atgggatcct ctatgcacag gatatgacat gtccttgtta tgtttatctt atttattata
     9361 gcggcaagag tacttgattt tccacttcca gtaggacctg ttacaagtac aagtccgttt
     9421 atataatcac aaactttttt tgtactttcc ggaaggttta atatttcaaa atcaggaatt
     9481 gtagatgtta ttcttcttgc aacaagagca aatgttcctc tctgtttgaa tatatttact
     9541 ctaaatctac caatatttgg aaaactcatt gaagtatcaa gattccctgt ttcttcaaga
     9601 attttcttct ccttttcctg taaaaattca taaattattt ttccaatatc atcttcactc
     9661 aattttttat actcttctat tcttaaaatg tctcccttta tccttaatat tggtttagaa
     9721 ccaacctgta aatgaatatc tgatgcattt ctttctttag aaagttctac aaattcctga
     9781 aaagaaaatt ctttttccat ttttttcttt atgttataat ttttaataaa atgaaaataa
     9841 ttatagttta tttttcatta actggcaata ccagaaaact atcagtaaaa atttatgaaa
     9901 ttctcaaatc aaaaaatctt gatgtaagtt tgtttgaaat tgagagcaaa gaaagatgca
     9961 gttttttgaa aaattgtttt aatgcaattt ttgaaaaatt aataaaaata gataaagttc
    10021 cacatataga aaattatgat ttgcttttta ttggtacacc catatgggct ggaaaaataa
    10081 ctccacatat tagaagtttt cttgaagata ttgacctgaa aaataaaaaa gtttttttgt
    10141 ttacaaccta tggaagtggt tttttaaaaa ataaagcgat gaaagaattt atagaacttg
    10201 ttgaaactga aggtggtaaa attgttgata tgtttgaagt aagaggggaa aaaatagaaa
    10261 aatatatgga caatttaaaa gaaaaaattg aaaaatgttt gaaagaatta taacagagaa
    10321 atcaccttta ccggtcggac cttattctca gggaattaaa tggaaaaatt ttatctttat
    10381 ttctggacag atacccgttg accctaaaac aaatactgtt gttaatggtg atataactag
    10441 tcaaactaaa caggtgatag aaaatattaa aaatatttta actgaagcag gcgcagatct
    10501 tgaaaatgtt gttaaaacca ctgttttttt aaaaaatata aatgattttg aaaagatgaa
    10561 ttatgtgtat gctcagtatt ttaaaaataa accagcaaga acaactgtag aagtttcaaa
    10621 tttaccaaaa aatgttctta ttgaaattga ggttattgca ataattgagg agggaaaatg
    10681 agacagaaac ttttttttat atttactctc ggttttattt cgggcgttat atgttctact
    10741 aaaatatcca ggatgcagag agttgaattt gttaattggc ctcagatgaa atttaaaaat
    10801 attagaatat taatagctgg tacagaaaaa ttatctaatc agggttttgc gggagcaagt
    10861 ttaaaagaaa tggaaaatac aattattatt tttccaaata ttcctgatgg tgttgttttt
    10921 actaatgtgg atcagggatt tggggcagta aaagaaaata ttaagattta ctaccttgat
    10981 aaaaatttta aaatacttaa atatgatata atggaaaagg aaaaagggat ttcaatacct
    11041 actgaaaata cttttattgc agttgaggga ttgccataat tctatacatg aggtaaaata
    11101 aaaaaaagga gagaaaatga aaaaattatt gattttattg attttattga tttttaatac
    11161 aggattttct gaaacaaaag ataaatatat aagaagattt aaagttgcag aagatttgct
    11221 tgaatatatg ttaaaacagc cagatgtttc tattcctgta aatcttatca aagaatgtta
    11281 tggaattatt tttataagac agtttaaagg tggattttta cttgctggaa agggagggga
    11341 gggtattata atttcaaggg atagagaaaa aaggacatgg agtgctcctg gatttattgc
    11401 ttctattcaa gtaagttttg gatggcagat aggtgctcaa atgacagatt taatactttt
    11461 gataatgaat gaggatggtt tgagaatgct cacgacctca aaagttaaaa taggagctga
    11521 tattggagtg gctgcaggtc ctgttggtag agaatttgaa gggaaagtat ccccaggagc
    11581 aggaattctt gcatatggaa gaagcaaagg tgtttttggt ggtttatctc ttgagggagg
    11641 agctattttt tctgatgata aggcaaatga agaattttat gggaaaaagg gtttgaaatt
    11701 gaaagagata atttttgata aaaaagtttc tattccaaaa gaagcagaaa aacttataaa
    11761 acttttaaag gattatgaag aggcagaact tgttatagaa aaaaaagaga aaaaataaaa
    11821 ttgaaaaagt atatttttct tttaattatt ctcaccggat gtattttaaa tattccatct
    11881 attgaaaaag aaaaaattaa tatatttaaa gacagaaaag atgcaatttt ttctccatcc
    11941 ggagatataa ataattctga atttgcaatt tctcctaatg gtgaatttta tgcaaaagaa
    12001 atagaacctt ataatcatgg tagaatagga atatttttga aaaatggaga attaataaag
    12061 gaaattaatg tttttgatga aaatgaggca gaaaatgatt taaaagtact tgcgtggcat
    12121 cctgataatt ttgtgatagg tgttgtatat cataaagatg gcggttcaga aataaagatg
    12181 tatgaaattt ttacaggaaa acttttgaga aagataattt tagggaattt ttatcattac
    12241 tttgtttttg atgaaaatgg agaaaaaata tatctttctg ttgatggaga aaaaatagat
    12301 gaagtaaatt taagaaatac tgattttgtt ttcaattcag ggattaattt accctggtta
    12361 aattatggat gggatattgg tagaaatcca tgggaagata agcatggtgg tttttcttca
    12421 aataaagaag ttcttaatga aaaatttaaa ttttttaaag agattggagt gaatgttgta
    12481 agggtttttt tgttctgtga tttaagaagt ggagttattt ttgacaataa tagattaact
    12541 tttgataaat atgtttataa agattttaat acactgattg agattacaga aaatatagga
    12601 attaaaattt tgccagttct ttttgatttt acaattgctg atggagtaga agaagagaat
    12661 ggaattaagg taggagagca tcccgaaatt ttttatgata taagagtaca gaaagagtta
    12721 ctgaatcttt ttgaaggttt ttttagaaaa attgagacga agaaagtaat ttatggatgg
    12781 gatattataa atgaaccaga acatttaaaa gtggacagtt ccaaagttga taatttcatt
    12841 tcagcattta ttaaattaat aaggacaatt aaaagaaatg aaaaaataac ggttggcgca
    12901 ctttcaagag tttatcttag taaatacaaa aaattcaatt tagatttcta tcaatttcac
    12961 tattatgaca gttttgaaaa caactcatca cttgattatc atttttataa tcttttaatt
    13021 gaaaaaccag tcattatagg agaattagaa ccgacagaaa ttccagataa acttacaaaa
    13081 atttgggaaa gaggatataa aggagttctt atatgggcta atgaagagtt tttaaaagat
    13141 ggatggagat tatataaagc gtggattgat gttcattgat tattgatgat aaaataaaaa
    13201 tatgttaaga agaaaagcaa gagagtatgc attgaaaatt ttatatatga tagatatgag
    13261 agaagatact aattctactt ttcttgaaaa ttttgaggaa aatgaaaaag taaaaaagtt
    13321 tgctgatgaa ataataaaag gagttattga aaatatgaaa gaaatagatg agataatttc
    13381 aaaagtttct ttaaactggg atataaagag aatgagttat attgatagaa atattttaag
    13441 aattgcaaca tatgaaatcg ttttcagaga agatatccct tcaattgtaa gtataaatga
    13501 agcgattgaa ataagtaaaa aatatggaga cgaagattct ccaaaattta taaatggtat
    13561 tttacacaaa ataaaagaaa tatatgaaaa taataaaaaa gaaaaaaagg gggatgaaaa
    13621 tgaaatttaa aattttttta tcttttttat ttttgatttt tttaattaca aatctttctg
    13681 gaaaagaaaa aaaagtttta atggttattg cttttgaaaa tttcagagat gaagaactta
    13741 aagttccaaa agaaatactt gaaaaaaatg gaattattgt tgacattgca tctgttaaaa
    13801 aaggtattgc taagggtatg cttggaacaa aaatagatat taatcttaca cttgaggaga
    13861 taaaagagaa tgattatgat ggaataattt ttgtaggagg tattggttcc cagagtttat
    13921 ttacaaatac acatgcaata aaacttgctc aaaactttta taaaagtaaa aaagttgttg
    13981 gagcaatatg tcttgctcca gggattttag ccaaagcagg tattttgaat ggaaagaaag
    14041 cgacctgttt ttacactgct tcagaaattt tgaaaaaaaa tggggcaatt tatacaggaa
    14101 atctggtaga aattgatgaa aatattgtaa caggaaatgg tcctgaagca gcagaaaagt
    14161 ttgggaatga aattttaaga ttaataaaaa agtgaaaaaa aatgttattt ttacaattgt
    14221 tagaatactt ataagtgttt tttttattta cttaatttta caaaaagtca attttaagaa
    14281 aatttctgaa attataaaaa atgcaaatta tttaccccta atatttgctt ttattacaat
    14341 attttcaata aattttttac ttgctctgag atttaaatat atactggaaa tttatttcaa
    14401 aaaaaaactt tcatattttt tgatttggaa acttacaatg ataggtttat tttttaataa
    14461 ttttcttcca acatcagcag gtggcgatat cgtaaaaatt ttttatattg tgaaagagca
    14521 ggaaaaaaaa tttttaagtg gcatatcagt tttaattgat aggtatatag gggctttatc
    14581 tattatgata atgggaacaa tttctgtatt tttttataaa ggaattgaaa aaaaatttca
    14641 gtacataatt tttgtttttc tgtttttttt aatttttgct tttttctttt tttctaaaag
    14701 aaaaattgct tcactttttt attctccaat taaaaagttt atacccgagt ttttaaaccg
    14761 taaacttcta aatctctata gttcaattta cttttatttt acagaaaaca gtaaaaactt
    14821 gaaaatagca attttgataa gttttatttt acagattctt tcaatttttt ctcaatatct
    14881 tataggagtt tcaataatca aaagtaattt aaatgttcta cttttttttg tttttattcc
    14941 tttgatatgg acatctactt taattccatc cttaggagga cttggagtta gagaatttac
    15001 ttatattttt ctattttctg attttattgg tgaacagaat gcttatgctc tttctatttt
    15061 agtacttacc actgttgttt tgaatagttt aataggtgga tttatttttt taacctttga
    15121 aacaaaaaga aaactaacag agaaaaatta ttaagtgaag agtttattga aaagatatat
    15181 taaaatctgg gttttcataa attcttacat atttaagaac cttttttatt cttgaaagtg
    15241 aaggcatttt ggttccttct tcttcaactt tagctgtacc acaggcaacc gcaaatttta
    15301 gacattcttc ataagttttt tttcttttta atttatatat gaaaccggat aagaaagcat
    15361 ctccacatcc aatactactt ttaaaatcaa cttctggtgg ttttgaataa aaaagtttat
    15421 cttttgtaaa taatattgca ccatctttac cgagagtaat aagtacaatt gaaatatcgg
    15481 aatttattaa agaaatacat atatttttta aagtttcaaa atttttaatc tttgtatcaa
    15541 aacatctttc aatttcctgt aaatttggtt ttattaaaaa gggtttttct tttatacttt
    15601 ctctcaaaac atctccgtca gcgtcaacta ttgttaaaac ttcaaatttt tttacccgtt
    15661 ttataatctc tgcataaatg ttgtttttaa taccaggagg gatactacct gacattacaa
    15721 caaaatctcc tttttttaaa tttaaattca ataaaatctc ccaaaattta tttatttctt
    15781 tattagttat caaaggtcct ttttcattaa atcttaaaac ttttttttct gatataaaat
    15841 tataaatatt tctcaaatta ccctttatca taataggtac aattttaatt ttttcttttt
    15901 taagtagttt tttatacagt ctaccattat tcttactgag aaaggttatt gtgataggtt
    15961 tttctccaag ttttcttaac attcttgaaa tattcatacc ctttccacct ggatcaattc
    16021 ttgttttttc aattctgtta acatcatcat ctattatatt gcttaaataa aaagaataat
    16081 caatacttga attcaaagaa attgtgtata ttaaggaagg catttttatt tacttttaat
    16141 aatggtattt aaatgaggtc tgtatttcct catttttttt ctttttctta cttcactcgg
    16201 tttttcataa aactcccttg ccttgatttc ttttattatt ccttctctct ctacctccct
    16261 tttaaattgt ttaaatattt tttcaaaatt tgtattattc actatacaat tcacctcctt
    16321 actttttata ttaggttttt aatgaattaa atactatcta ttttataaat tttagcacca
    16381 cagtgattac attttatttc tttgccttct tctattatat aactatcaac cataaaacat
    16441 tttctgcatc ttactgttat ttttggcttt gttcttgttt tcatctttac ctcttttttc
    16501 ataaatttat tataactttt tctactattt ttgtcaattt tggttctgca gaatttgcaa
    16561 cttcaactat ttcctcaaaa gatactggtt ttaacgcatc tggtaaacac atatctgtaa
    16621 taacagaaag tcccaatatt ttaagtccaa gatgaaccgc tgcaataact tctattactg
    16681 ttgacataca taccatatcc gcaccaatac ttcttaaaaa cctatattct gctttagttt
    16741 caaaatttgg accaagaaga ccaacaagaa cacccttttt taaaggtatt ttttcttcta
    16801 tccctacttt ttcagcaatt tcaatcaaat caggtgaata aggagaaaac atatccggaa
    16861 atcttggacc aaatctttcg tcattttctc caacaagagg attctgtcct gtaagattta
    16921 tatggtcaac tattataaca atatctccct ttttataaag cggatttaaa cctccggcag
    16981 catttgactc aattaaaata tttattccaa gcaatttcat aactcttatt ggaaaaacaa
    17041 cttgatatgg agtatatcct tcatataaat gaaatcttcc ctgcattgct actacatttt
    17101 tattacctat tttccctata acaagatttc caagatgtgt ctcaactgta gacataggga
    17161 aataaggtat ttctttgtaa gggataatta tactttcttc tattttttca gcaagagcac
    17221 ccaatccagt tccaagaatt attccaatat ccggttttac tttaatttta cttcttataa
    17281 actctgctgc ttcttttgct ttttcaaatt ctttcatttt tccctcctag ttaaatttgg
    17341 ttaaaaatat attattatat aacttaaact ctttgaaaat taaattttta aagaacaaaa
    17401 aggagagaaa aacatcattt ttcctgggca ataaggctca caatagaaca tgttttctct
    17461 ccctttcgtc ccatattggt tgattgggta tttttgaccc atcttacccg ggcacttggg
    17521 acaaagggta tctcctctcc ctaaaataaa ggaggtgata tcatgaaaaa gtttaattat
    17581 tctatcggaa tagatgtaag taaagatagt tttgtcgtat ctattaaagg taatgatttt
    17641 attaagtcca atctctcttt ctctatggat aaatctggct ttgaaaattt tgcctctcaa
    17701 actattcagt ttaaaaattc ttctgtctgt gcttgtgaac ctaccagtat ttaccacata
    17761 aatcttgtta atttcctcaa agaaaaaggg tataatgtct gtatggttaa tcctt
//
LOCUS       DAOSAT010000027        17813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2200_length_17813_cov_5.352010, whole genome shotgun sequence.
ACCESSION   DAOSAT010000027 DAOSAT010000000
VERSION     DAOSAT010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17813
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2200_length_17813_cov_5.352010"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..869)
                     /locus_tag="PLF90_03595"
     CDS             complement(84..869)
                     /locus_tag="PLF90_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOK56402.1"
                     /translation="MKIIVCIKQVPEGLDVKVDPETKRIVREGVKSIINPYDLYAIEE
                     GIRLKERFGGETWVISMGPPQAESAIREAISMGIDNGVLISDRKFAGSDTLATSYTLS
                     LGIKKIGEFDIIVCGRETLDGSTGQVGPGIAEHLGISYITYVSKIVDIKDNEFECIRL
                     MEDHYEIVKTKLPVLITVVKEINVPRIPSLKNMMRAKKIEIPVWKAENLGGDESLFGQ
                     DGSPTRVIDVWIQTITKEGRKVEGEPEYLVEEIINELKKLGVI"
     gene            complement(912..1889)
                     /locus_tag="PLF90_03600"
     CDS             complement(912..1889)
                     /locus_tag="PLF90_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK56403.1"
                     /translation="MIKIGFVGCGGISYRHLNDLKNMKDVKIVSAIEPNEDNFKKFQD
                     TYGEKLNLYKDEEEMIEREKLDGVVICTPHTLHFSQIKIALQKGIDVLVEKPAVVTYE
                     ESIEIRKLIEKTGKNVVVAYQRHYMPVFNGARKIIKEKFGNIIFLSGFLAQYYYEPIS
                     TRRPWRINPQLSGKGQLTDSGSHFVALLFFLTGLTPEKVASFIDFRGEKVDMNSAFIV
                     KFKEGAIGNFGILAFDPSFRENLFIWDDKNNVLKVSAMENSYAQFKGEKETKNIEGLE
                     IDVKNPSEDLIKCIKKEKKTHTDWKIVENVSLLSDMVYKAYFESKVLEF"
     gene            complement(1905..2756)
                     /locus_tag="PLF90_03605"
     CDS             complement(1905..2756)
                     /locus_tag="PLF90_03605"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOK56404.1"
                     /translation="MEFNNPFLKEGKWFKGNAHIHTTNSDGKLSPEEIVEIYRENFYN
                     FLFITDHNKITDYQSPYEDFLVIKGAEYSKNGFHILGLGLKENFNTENLSPQEIINKI
                     NELDGFVVVCHPYWSGITSMDILSLTGFTGIEIFNNSCEIEKGKGYSTVHYDEVLQKG
                     MKVSGFAVDDSHSKKDIIGSFIMVKAKSLDTEEICASIKDGFFYSSTGVIINDLKFKD
                     DIIRISFTPSITVDFIGYGASGTRVYNNGREFDYAEYRIKGPEKYIRIEITDKENKKA
                     WVNPFFL"
     gene            complement(2766..4460)
                     /locus_tag="PLF90_03610"
     CDS             complement(2766..4460)
                     /locus_tag="PLF90_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56405.1"
                     /translation="METKVIIDSLKRVQYSRCEKGIFSAIEHFGFYYKIIDLAFERFF
                     EREIENTNLIIIGQEGLGFSFGEIETDIILKNLYSGVGVVIFDGYISSYPSSFLKALK
                     IGNLTPKMTSKIKLEKTAWICEGTYCDEVVLKNEIMYYSAEVEKKFWKPFLYSENGEI
                     CGIYGRFGKGKIVLFFTSAGLWQDEILGHTEGLDDVFFRSLIWASKKPFITKTIPPFI
                     TARIDDVSASGSRVARYKETVEKFRYVDVLNKYKIIPNLGLFIDDIKEDDLLPLREKY
                     FEKLAEFSPHAFSDPNNINEYPIYMKHNGEEFSDNEIKENFYRVDKKFEYFGIKPSIT
                     LNAHFGEIGLKSLPYLKERNQNFLMNIIKIGKAYSDPKAHIWDLKPYGRINFSLSEIP
                     EDNYFFNVLSIPLKIEENLYDEKKVDFDFLYKCTNFWNENEKTDIKRAIKRGIFQIKR
                     GLENKFFGCLMTHEQRISFLKIEEWEEIVRGIISSIGIKNKIFKSYDYISLYSRNLKA
                     ISIEKIELDKNLQIILRGKTKISLYLYIFYDRDEEVIENFLEIPPFENYTTLNFKI"
     gene            complement(4460..4972)
                     /locus_tag="PLF90_03615"
     CDS             complement(4460..4972)
                     /locus_tag="PLF90_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOK56406.1"
                     /translation="MEKVINEVLNVFKKIKKEEFEEFEKVILKSKKIFVFGTGRSGFI
                     GRCFCMRLFHLGFDSYFVGEIITPAFTEEDLIIFISGTGEKSIVLQIAKICRKEKGRI
                     LTITANKKSKLSKISDYLIYIPVENSIQFGNSLFEQVCFLFLDSFIQFYIEKHRINED
                     LMKKRHTNLE"
     gene            5401..6861
                     /locus_tag="PLF90_03620"
     CDS             5401..6861
                     /locus_tag="PLF90_03620"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK56407.1"
                     /translation="MALKLEDRILTIMEKGTSQKVVTGILNASLKNSVTRRALINAID
                     RATYKMSLKTYERPKKVLEERYYMGRALLHSIERLFDTDSISPNCVRALSNIFLGKIF
                     IQGIYTRRTFEEKHGFRPPCFVTISPTNVCNLKCKGCYAGEIYEKHTLKFDVFDRIIK
                     EIKEEFGAYFFVISGGEPFMYKDNGKTLFDILEKHNDAYFMAYTNGTMIKKDVAKKLS
                     ELGNFTPAISVEGFESQTDDRRGNGVFKRIMEVMDNLREEGVPFGISVTPTRYNADLL
                     LSDEFIDFYFEKKGAFYGWYFQYMPIGRSPSVELMVTPEQRYKMLKRIWQLVIEKKIF
                     IADFWNSGTASDGCMAAARGGGYFYIMWDGTITPCVFIPFKDKNLGNIYEIYKEGKTI
                     TDAIKSPLFERIRNWQYNYWIKRNKNECGNLLVPCIIRDNSLDFYKIVKETDAQPVDE
                     GAKDYLKFIEEGKMPEYNKKYRELTDPLWQNEYLNE"
     gene            6854..8467
                     /locus_tag="PLF90_03625"
     CDS             6854..8467
                     /locus_tag="PLF90_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOK56408.1"
                     /translation="MNKHITVIRKIKRYRRIVNILIKYGFEILVERAHIFKFPFRKKR
                     QIELTFPVRVRKILEELGPTFVKLGQILSTRPDLIPFEYIRELEKLQDEVKPENFDLM
                     KEIIEKETGGKLEEIFDVFDKEPIASGSLSCVYKAVYKGKRVAVKVQRPYIKEQIFTD
                     IQILYDIANLIEKFIKESEIYQPVKIVKEFEKSIKKELNFLIEGKNIEIMKERMKNFE
                     KLYIPEIYKEISTEKLLVTEYIDGIKINRVEDWSKFVSKEEVLKNGADIILKQIFYTG
                     IFHGDPHPGNIFIMRDGKIALIDFGIVGHLDEERKYYIVNLISSILKGNTEKVILTLK
                     LMGSIGDTTDIQELREDIEDLIEVYRDIPIKKIKIGEVLGICFEIMRKNRIKIPVSFT
                     LMGKSIITIEGICHFIDPEFKLTESIEPFFIEITEKKVKFSYFLKEFQNTFNSLHYVI
                     KELPQTIETLMDTVKNSKRKDYRNENTTENLNSTVKKAGIKISFSLIISTFLISSVFL
                     FISGYFLFGISEFVITLIMLLVFFLKMRD"
     gene            8468..8914
                     /gene="smpB"
                     /locus_tag="PLF90_03630"
     CDS             8468..8914
                     /gene="smpB"
                     /locus_tag="PLF90_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOK56409.1"
                     /translation="MKIENKSAYHDYEILERIEAGIVLKGSEVKSIREGKIAFKDSYA
                     KVENGEVFLYNFYIAPYQSSFEKINPLRKKKLLLNKNEIRRLEKRVSEKRLTLVPLSV
                     YFNNRGLAKIELAVAKGKKLFDKRRELKEREIERDIARKMKNMGAK"
     gene            8902..9254
                     /gene="ssrA"
                     /locus_tag="PLF90_03635"
     tmRNA           8902..9254
                     /gene="ssrA"
                     /locus_tag="PLF90_03635"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            9519..10619
                     /locus_tag="PLF90_03640"
     CDS             9519..10619
                     /locus_tag="PLF90_03640"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOK56410.1"
                     /translation="MFLKNIRFLIFFILLTSLSIYSLNLEEEIKTRKIPTYLIKDVQY
                     ISLKSFLKIINSDNWAKIEDRIFVFFDGTQIKFRIDDKTLIVGGKTIEIKNTPKEIEG
                     DILIPVEDFSKIFADFKISEKKEEREKTSEEKKESRKFIIVIDPGHGGHDSGAIGNYG
                     LKEKDVNLDVSLRLEKYLKKQLESYPSIEIYLTRKEDVFISLEERVNIAKTLNADIFF
                     SVHTNSSRFNRFDATGFETYYPYCKVETNSLPKSNDGEGELKEENNDSVLNQILNNLN
                     ETDIIDEGRILAECVQEKLAERLITPDRGAKRGNFYVLKYTPMISVLTEIGFICNPNI
                     EFNLKDVEVRQAIAETLGKAIIEYLKKREIIK"
     gene            10616..11413
                     /gene="murI"
                     /locus_tag="PLF90_03645"
     CDS             10616..11413
                     /gene="murI"
                     /locus_tag="PLF90_03645"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOK56411.1"
                     /translation="MNKNPIGIFDSGVGGLSVVKEIIKVLPEENIIYFGDTARVPYGT
                     KSERVIKKFAVEDVIFLLKYNPKLIVIACHTVSSYASYYLRKKFKNIKIIDVIEPTVE
                     KAVKLTKNKKIGVIGTTATISSLRYQKLFSKYKGIEVYTKDCPLFVPVVEEGWFENEI
                     SYKIAEIYLSELKKKNIDTLVLGCTHYPYLKKTIQKIMGKNVEIIDASYEVSIKIKNI
                     IEENNLNNKGGKGKISLYFSDISPHVEKTIKFLFEKNNIKYKLTKNV"
     gene            11406..11774
                     /gene="queD"
                     /locus_tag="PLF90_03650"
     CDS             11406..11774
                     /gene="queD"
                     /locus_tag="PLF90_03650"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOK56412.1"
                     /translation="MYELKINGEFSSAHSLRNYKGRCENLHGHNWKVEIIVTGETLDR
                     IGLLIDFNILKKKLNIILKMLDHKNLNSIPFFIERNPSSENIAFFIYKKMERLLRGYP
                     VRIQKVTVWENEKQCASYYE"
     gene            11743..12252
                     /locus_tag="PLF90_03655"
     CDS             11743..12252
                     /locus_tag="PLF90_03655"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOK56413.1"
                     /translation="MKNNAQVTMNKNYWIVEIFKKRNIRDVFGEDVKKSIEELGISDI
                     EEVKVSNLYKIECDTEKKNIEKIAKELLIDNVSEDFLIYKKSKTKKNYWIVEVFLKEG
                     VTDPVGETSKKTIIESGILKDVNVKTGKKYYIKGKLQEKQIKEICKKILANTLIHNYF
                     IFKKEGMGK"
     gene            12249..14615
                     /gene="purL"
                     /locus_tag="PLF90_03660"
     CDS             12249..14615
                     /gene="purL"
                     /locus_tag="PLF90_03660"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOK56414.1"
                     /translation="MKHAKEIDILNADDKTLIEISEKNLLSLDIGEMRKIKEYFLSIG
                     RNPTDCELECLAQTWSEHCCHKTFKANINYVEKIGEKEEKINICLLKEIMEVTEKLNY
                     DWCISVFKDNAGIIELNEKWGVAFKVETHNHPSALEPYGGAGTGIGGVIRDILGVGLG
                     AKPILNTDVFCFGPLDYSYEKLPEGVLHPRRVFRGVVSGVRDYGNRMGIPTGNGAIIF
                     DDGYVYNCLVYCGTVGILPVDKVEKKVKDGDFIVVIGGRTGRDGIHGATFSSASLKED
                     IPTSVVQIGNPIVEKKFQDVLLVARDLNLYNSITDCGAGGFSSAIGEMGKDTGAVVYL
                     DKVPLKYEGLLPWEIFLSESQERMVLAVPEKNIEKLISLFNSEDVEATVIGKFIKTGK
                     LTVYYKDEIVCDIDMKFLHKGIPERNLKCFFEKKEEKKLNFKSKNLKEDILKLISTPV
                     ISSKEEVVHQYDHEVGGRTVLKPLLGYESRGVSDGVVLKPFYETYEGLVIGCGINPFY
                     GEIDPYYMAGCCIEECIRNIVSCGGNPEKIAILDNFCWGNVSDEKELGKLVRCVKGCK
                     DYALKYKVPFISGKDSLNNYFRGKNGKIISIPGTLLISGIGVIEDVRKITSTEFKKGR
                     NIIYVLGKTFNELGSSQFYRMYGIKGGIVPKPRPEKTLNLMKKIHTGIKEGLIKSCHD
                     CSDGGLITTINEMMIGGNLGCEINFLDVLSEEKEPEIILFSESCGRFVVEVEKEKINE
                     FEKLFKGEEVSQIGQVIEKKEMIGFWGNKKLFKISLDLIYENWRSLKF"
     gene            14618..15382
                     /gene="purQ"
                     /locus_tag="PLF90_03665"
     CDS             14618..15382
                     /gene="purQ"
                     /locus_tag="PLF90_03665"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOK56415.1"
                     /translation="MKPKVIILRVAGTNCDFETEWAFNLAGASAERIHINQIIRKEKK
                     LSDYQILVIPGGFSYGDDIGAGKVLANQIKLKLWDEVEKFIFEKKVILGICNGFQVLV
                     KSGILPFNSEQSVTLEWNDSGRFEDRWIYLKVEKSICPFFEGLPDIIRMPVAHAEGKF
                     YPKNSKSLKEIEKNKQVVLRYCDKNGNLSGYPYNPNGSIGNIAGICSKDGLIVGMMPH
                     PERAILKYYYPDFLKNYGDEFTPSFKIFKNMVKYFS"
     gene            15427..16305
                     /gene="argB"
                     /locus_tag="PLF90_03670"
     CDS             15427..16305
                     /gene="argB"
                     /locus_tag="PLF90_03670"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOK56416.1"
                     /translation="MTDEVGKKADILVEAFKYIKEFKNRIFVIKVGGSIYQIEDVKES
                     ILFDVAFLSFLDIKIILVCGGGKFINDEIEKKGGKAKFVDGLRVTNLQTLNIVKDVLF
                     RVRDEIVTILKDKFECNVDKITPEEKFVTASKIHYQKGEEVVDLGFVGQLKKANIEYI
                     ENKLKEKNILVCAPLAMGDDENLYNVNGDTFASFVASNLKSEKLIFITDVLGIMRNPE
                     NPDTLISTLTVSQGEELIKDGVIKEGMIPKVKAGINGIKNGVKSVHIISGNIPHSLIL
                     EVLTEHGVGSEIIKNG"
     gene            16298..17488
                     /locus_tag="PLF90_03675"
     CDS             16298..17488
                     /locus_tag="PLF90_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007044676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOK56417.1"
                     /translation="MDKEKILKNYNDYVIPSYRKQNFIFVKGKGSYLWDIDGKKYLDF
                     FPGWAVSGLGHCHPQVVKKIKEQVEILIHISNNYYHPWQALLAEKLSRLSFGGKVFFC
                     NSGAEANESAIKLTRLYGRKKGKYKIISMENSFHGRTLATVTMTGQKKYNEMFEPLPP
                     GFIYAKFNDFSDLYSKIDDEVAGIILELIQGEGGINVVDKDYLKKVKDLCEKKDILLI
                     FDEVQTGFGRTGELFAYQIFGVEPDIMTLAKTLGGGFPVGAMIARKEIADLMGPGTHA
                     STFGGSPLACAACLGVLEAIENENLLENVKIMGKYLKEKLEKLKEEFKGIKKVKGLGL
                     MLAIEIEKQGEELVNICAKNGLLINCTHQNVIRIMPAINVKKEEIDEGIEILKKSLKE
                     AGIQ"
     gene            17485..>17813
                     /locus_tag="PLF90_03680"
     CDS             17485..>17813
                     /locus_tag="PLF90_03680"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF014749.2"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOK56418.1"
                     /translation="MKRDFLKIKDISKEEVYKIFDITKYFKSLKKEKKITEHLKNLTI
                     GLLFSKPSTRTRISFEVAIQQLGGFPIYLSSEVTQISRGESIKDTAKVISRYLDGIII
                     RTYAQEEV"
BASE COUNT         6691 a         2388 c         2893 g         5841 t
ORIGIN      
        1 ttttatttca attgcaccaa aaggacattt ttcaacacat tttttacata gatttcattt
       61 ttcaaaaaga atctttatac ccattatata actcctaact ttttaagttc atttataatt
      121 tcttctacga ggtattcagg ttctccttca acttttcttc cctcttttgt tattgtttga
      181 atccaaacat caattactct tgtaggtgaa ccatcctggc caaataaact ttcatctcca
      241 ccgagatttt ctgctttcca gacaggtatt tcaattttct ttgccctcat catatttttt
      301 aaagatggaa ttctcggtac atttatttct ttaacaactg ttattaaaac tggtaatttt
      361 gtttttacaa tttcataatg gtcttccata agccggatac attcaaactc attatcttta
      421 atatcaacaa ttttacttac atatgtaata taagatattc caagatgttc agcaattcca
      481 ggaccaacct gacctgtact accatcaaga gtttcccttc cacatacaat aatatcaaac
      541 tcacctattt tttttattcc aagtgataaa gtatacgaag ttgcgagggt atcacttcca
      601 gcaaattttc tatcacttat aagaacccca ttatcaatac ccatacttat tgcttctctt
      661 attgcacttt ctgcctgagg tggccccatt gaaattaccc atgtctcgcc tccaaatctt
      721 tctttcaatc ttattccttc ttcaattgca taaaggtcat agggatttat tatacttttt
      781 actccttctc ttacaatcct ttttgtttca gggtcaactt taacatcaag tccttctgga
      841 acctgcttta tacatacaat tattttcatt ttttaaactc catttttaaa aaaataattt
      901 tatcataaat tttaaaattc taaaactttg ctttcaaaat atgctttata aaccatatca
      961 ctcaataaag acacattttc tactatcttc cagtcagtat gtgttttttt ttcttttttt
     1021 atacatttaa taaggtcctc tgatggattt ttaacatcaa tttcaagtcc ttctatattt
     1081 tttgtctctt tttctccttt aaactgcgca taagaatttt ccattgctga tacctttaaa
     1141 acattatttt tatcatccca tataaaaagg ttttctctaa aagatgggtc aaaagcaaga
     1201 atcccaaaat ttcctattgc tccttcttta aacttcacaa taaacgcaga attcatatca
     1261 actttttctc ctctgaaatc aataaatgaa gccacctttt caggagtaag tcctgtcaaa
     1321 aagaataata aagcgacaaa atgacttcca gaatcagtta actgtccctt cccagaaagt
     1381 tgaggattta ttctccatgg tcttcttgta cttattggct cataataata ctgagcaaga
     1441 aaacctgaaa gaaaaataat atttccaaat ttttctttta taatttttct tgcaccatta
     1501 aaaacaggca tataatgtct ttgatatgca acaacaacat tctttcccgt tttttcaatt
     1561 aatttcctta tttcaatact ttcttcataa gtaacaactg cgggtttttc tacaagaaca
     1621 tctatacctt tctgaagtgc aatttttatt tgagaaaaat gtaaagtatg gggagtacat
     1681 atgacaacac catcaagttt ttccctttca atcatttctt cttcatcttt atataaattc
     1741 aacttttctc cgtaagtatc ctgaaatttt ttgaaattat cctcatttgg ttcaatagca
     1801 gaaacaattt taacatcctt catatttttt aaatcattta aatgtctgta agaaattccg
     1861 ccacatccaa caaatccaat ttttatcatt tttttcctcc ttatttataa aaaaaatgga
     1921 tttacccatg cttttttatt ctctttatct gttatttcaa tccttatata tttttctgga
     1981 ccctttattc tgtattctgc ataatcaaat tctctaccat tattataaac tcttgtgcct
     2041 gacgctccat aaccaataaa atcaactgta atagaaggag taaaagaaat tctaattata
     2101 tcatctttaa atttcaagtc atttataatt acacctgtgg aagaataaaa aaatccatct
     2161 ttaattgagg cacatatttc ttctgtatcc agagattttg cttttaccat tataaaacta
     2221 ccgattatat cttttttgct atgactatca tctacggcaa atccagaaac tttcatacct
     2281 ttctgtaaga cctcatcata atgaacagtt gaataacctt tccctttttc tatttcacaa
     2341 ctattattaa atatttcaat acctgtaaaa ccagttaaag aaagaatatc catacttgtt
     2401 atcccgctcc agtaaggatg acagacaaca acaaaaccat caagttcatt tattttattt
     2461 attatttcct gaggagacag attttcagtg ttaaaatttt ctttcaaacc aagcccaaga
     2521 atatgaaaac cgtttttgct atattctgct cctttaatta caagaaaatc ttcgtatgga
     2581 ctttgataat cagttatttt attatggtct gtaataaaaa gaaaattgta aaagttttct
     2641 ctataaatct caacaatttc ttctggagat aattttccat ctgaatttgt cgtatgaata
     2701 tgagcatttc ccttaaacca ttttccctct ttcaaaaaag gattattaaa ttccatattc
     2761 cctccttaaa ttttaaaatt taatgtagta taattctcaa aaggtggtat ttcaagaaaa
     2821 ttttcaatta cctcttcatc cctatcataa aaaatgtaca aataaagaga aatttttgtt
     2881 ttacccctta atataatctg caagttttta tcaagttcta tcttctctat tgaaattgct
     2941 tttaaatttc tggaatataa acttatataa tcataactct taaaaatttt gtttttaata
     3001 cctattgaac ttattattcc ccttactatt tcttcccact cttctatttt caaaaaagaa
     3061 atcctctgtt catgtgtcat caaacatcca aaaaacttat tttcaagtcc tcttttaatc
     3121 tgaaagattc ctcttttaat agccctttta atatcagttt tttcattctc attccagaaa
     3181 tttgtgcatt tatatagaaa atcaaaatct acttttttct catcatataa attttcttct
     3241 attttcaaag gaattgataa aacattaaaa aaataattat cttccggtat ttcactcaaa
     3301 gaaaaattaa ttcttccgta aggttttaaa tcccatatat gtgcctttgg gtctgaatat
     3361 gcttttccta tctttataat attcattaaa aaattctgat ttctttcttt taaatacggt
     3421 aaggatttaa gtccaatctc tccaaaatga gcatttaaag ttattgatgg ctttattcca
     3481 aaatactcaa attttttatc aactctataa aaattttctt ttatttcatt atctgaaaac
     3541 tcctctccat tatgtttcat ataaattgga tattcattta tattattcgg gtctgaaaaa
     3601 gcatggggtg aaaattctgc cagtttctca aaatattttt ctcttaatgg taataagtca
     3661 tcttctttta tatcatcaat aaatagtccg agatttggga ttatcttata cttatttaaa
     3721 acatctacat atctgaattt ttccactgtt tctttatatc ttgcaaccct acttcctgac
     3781 gcagaaacat cgtcaatcct tgcagtaata aaaggtggta ttgtttttgt tataaatggt
     3841 tttttacttg cccagattaa acttctgaaa aaaacatcgt caagcccttc tgtatgtcca
     3901 agaatttcat cctgccacag acctgcagaa gtaaaaaata aaacaatttt accctttcca
     3961 aatcttccat aaataccaca aatttctcca ttttcactgt aaagaaatgg tttccagaat
     4021 tttttttcaa cttcggcaga ataatacatt atttcatttt tcaacacaac ttcatcacaa
     4081 taggtccctt cacaaatcca cgctgtcttt tcaagtttta tctttgatgt cattttagga
     4141 gttaaatttc ctattttaag tgcttttaaa aaactcgatg gatatgaaga aatatagccg
     4201 tcaaaaataa caactccaac acctgaatag agatttttta atataatatc tgtttctatc
     4261 tctccaaagg aaaaaccaag tccttcctgt ccaataataa ttaagtttgt attttcaatt
     4321 tctctttcaa aaaatctttc aaatgcaaga tcaatgattt tataataaaa accgaaatgt
     4381 tcaatcgcag aaaaaattcc cttttcacat cttgaatatt gaactctttt taaagaatca
     4441 attataactt ttgtttccat tattctaaat ttgtatgtct tttcttcatc aaatcttcat
     4501 ttatcctgtg tttttcaata taaaactgga taaaactatc aaggaataaa aaacatacct
     4561 gttcaaataa ggaattccca aactgaattg aattttcaac tggaatataa attaggtaat
     4621 ccgaaatttt tgaaagttta ctttttttat ttgcagttat agttaaaatt ctcccctttt
     4681 cttttctaca tatttttgct atctgtaaaa caattgattt ttctcctgtt cctgaaataa
     4741 aaattattag gtcttcttct gtaaaagcag gtgttatgat ttcgcctaca aaataggaat
     4801 caaaaccaag atgaaacaat ctcatacaga aacatctccc aataaaacca cttcttcctg
     4861 ttccaaaaac aaaaattttt ttactcttta aaattacttt ttcaaattct tcaaattcct
     4921 ctttctttat ttttttaaaa acatttaata cttcattgat taccttttcc atttttaaaa
     4981 ttttaacact aattataaaa ctttcaatca taaaattaaa tcaggatttt atagcaaact
     5041 atttttgtgg gtaaatactt actgaaccac atacataaaa cactggaatt tgaaaaattc
     5101 caagtgtttt ttctggcgtt ttttgcccag aacaagttat aattacctta aaaccctcaa
     5161 agattgatgg ggtgctggtg cgacgaataa ggagcaccga ttagatgaga ggcgagatgg
     5221 tgagccaccc atttggggga gttgatacct cttggggatt ttaaaggggt taaacctctt
     5281 taagaaaata aagtagttga caagaaaaac tgaattataa caaaataaga aagtaaacgt
     5341 gtttcaataa aaatttactt ttgtggtata atataatttg ttatccacaa ggaggttaaa
     5401 atggcattga aacttgaaga tagaatatta acaattatgg aaaagggaac ctcacagaaa
     5461 gttgttacag gaatattaaa tgcttcttta aaaaattcgg taacaagaag agcactgata
     5521 aacgcaattg acagagcaac atacaaaatg tctcttaaaa catatgaaag accgaaaaaa
     5581 gttcttgaag aaagatatta tatgggaaga gcacttttac attcaattga aagattattt
     5641 gacacagata gtatttcgcc aaattgtgtt agagcactct caaatatttt tctggggaaa
     5701 atttttatac aggggatata tacaagaaga acatttgaag aaaaacatgg atttagacct
     5761 ccctgctttg ttactatctc tccaacaaat gtatgtaatt taaaatgtaa gggatgttat
     5821 gctggagaaa tttatgaaaa acatacttta aaatttgatg tttttgatag aattataaag
     5881 gaaattaagg aagaatttgg tgcttacttt tttgtaattt ctggtggaga accatttatg
     5941 tataaagata atgggaagac actttttgat atacttgaaa aacacaatga tgcttatttt
     6001 atggcttaca ccaatggtac aatgataaaa aaagatgttg caaaaaaact gtctgaactt
     6061 ggaaatttta cccctgctat ttcagttgaa ggttttgaaa gtcaaacaga tgatagaaga
     6121 ggtaatggtg tttttaaaag aattatggaa gttatggata atttaagaga agaaggagtg
     6181 ccttttggaa tatcagttac accaacaaga tataatgctg atttactttt atcagatgaa
     6241 tttattgact tttattttga aaagaaaggt gctttttatg gatggtattt tcagtatatg
     6301 ccaataggaa gaagtccttc tgttgaactt atggttactc cagaacaaag atataaaatg
     6361 ttaaaaagaa tatggcaact tgttatagaa aagaagattt ttatagcgga tttctggaat
     6421 tcagggactg cttctgatgg atgtatggct gctgcaagag gaggcgggta tttttatata
     6481 atgtgggatg gaacaataac tccttgtgta tttattcctt ttaaagataa aaatttaggt
     6541 aatatttatg aaatttataa agaggggaaa acaataactg atgcaattaa gtctccctta
     6601 tttgaaagaa ttagaaactg gcagtacaac tactggataa aaagaaataa aaatgaatgc
     6661 ggtaatcttc ttgtgccctg tataataaga gataattctc tggattttta taaaatagta
     6721 aaggaaacag atgcacagcc ggtagatgaa ggagcaaaag attatttaaa gtttatagaa
     6781 gaaggaaaga tgcctgaata taataaaaag tacagagaat tgacagaccc cctctggcag
     6841 aatgaatatt tgaatgaata aacatataac agtcataaga aaaattaaaa gatacagaag
     6901 aatagtaaat atcctcatta agtacggatt tgaaatactt gttgaaagag cccatatttt
     6961 taaatttcct ttcagaaaaa aaagacagat tgaattaaca tttcctgtaa gggtcagaaa
     7021 aattcttgaa gaactcggac caacatttgt aaaactcgga cagattttaa gcacaagacc
     7081 agaccttatc ccttttgaat atataagaga acttgaaaaa cttcaggatg aagttaaacc
     7141 tgaaaatttt gatctgatga aagaaattat tgaaaaagag acaggtggaa aactggaaga
     7201 aatttttgat gtttttgata aagaaccaat tgcttccggt tcactatcct gtgtttataa
     7261 agcagtatat aaagggaaaa gagtcgctgt aaaggttcag agaccatata taaaagaaca
     7321 aatttttact gatattcaga ttttatatga tattgcaaac ttaatagaaa aatttattaa
     7381 agaaagtgaa atttatcagc cggttaaaat agttaaggaa tttgaaaaga gtataaaaaa
     7441 agaattgaat tttcttatag agggtaaaaa tattgaaata atgaaagaaa gaatgaaaaa
     7501 ttttgaaaaa ttgtacatcc ctgaaattta taaggaaata agcacagaaa aattgcttgt
     7561 tactgaatat atagatggaa taaaaattaa tagggttgaa gattggagta aatttgtaag
     7621 caaggaagaa gtattgaaaa atggagcgga tataatttta aaacagattt tttatacagg
     7681 aatttttcat ggggatccgc atccgggaaa tatttttatt atgagagatg gaaaaattgc
     7741 actgattgac tttggtattg ttggacatct tgatgaagaa agaaagtatt atattgttaa
     7801 tcttatttca agcattttaa aagggaatac agaaaaagta atattaactt taaaactcat
     7861 gggttctatt ggagatacga cagatattca ggaattaaga gaggatatag aagatttaat
     7921 tgaagtatac agggatattc caataaaaaa aataaaaatt ggagaagttc ttggtatctg
     7981 ctttgaaatt atgagaaaaa acagaattaa aatccctgta agttttactt taatgggtaa
     8041 atcaataatc acaattgaag gaatatgcca tttcattgac cctgaattta aacttactga
     8101 atctattgaa ccatttttta ttgaaattac agagaaaaaa gtaaaatttt cctatttttt
     8161 aaaagaattt cagaatacat ttaacagttt acattatgtt attaaagaac tgccacagac
     8221 aattgaaaca cttatggata cagttaaaaa ttcaaaaagg aaagattata gaaatgaaaa
     8281 tacgactgaa aatttaaatt caactgtaaa aaaagcagga ataaaaattt ctttttcttt
     8341 aattatttca acttttttaa tttcttctgt tttccttttt atatctggat attttctttt
     8401 tggaatttca gaatttgtaa taactttaat aatgcttctt gtattcttct taaaaatgag
     8461 ggattaaatg aaaatagaaa acaaaagtgc ttatcatgat tatgaaattc ttgaaagaat
     8521 agaagcgggt attgttctta aaggaagtga ggtaaagtca attagagagg gaaaaattgc
     8581 atttaaagat tcttatgcaa aggtagaaaa tggagaagtt tttctttata atttttatat
     8641 tgcaccctat cagtcctcat ttgaaaaaat caatcctttg agaaagaaaa aattgttact
     8701 gaataaaaat gaaataagac gacttgaaaa aagagtttca gaaaaacgct tgacacttgt
     8761 accactttca gtgtatttta ataatagagg tctggctaaa atagaacttg ctgttgcaaa
     8821 aggtaaaaag ctctttgaca aaagaaggga attgaaagaa agagaaattg agagagatat
     8881 agcaagaaaa atgaaaaata tgggggcgaa ataggaatcg acaggaagaa atccggatag
     8941 agttgcctgt caagtttgtc aggaacttgt aaaaacctga caaaaaataa ctgccgtaac
     9001 agcaaatact ctggctgcat aaaatgcagt cacccattta cagagtttgc ctctgactct
     9061 gtaaaatggg tcggctaaga ggcatcttct ttaagggtta tccttttccc ttaaagaaga
     9121 ttaataaaaa ggatgcgggt aaaagggcct gtcagaagtg cctttttacc ctaagaaaaa
     9181 acttctgact atacaggtag atactctatc aggattcttt ctggacgcgg gggcagtacc
     9241 cgccgcctcc acatattcag caaaaactaa aggaacaagg aaaactgaag catcagggct
     9301 taccagcgga cagaaaaagg gtttaagggg aaaaagattt ttccccttat atgttgtggg
     9361 ggtatcgtcg ggaaagggga atctcccctg tcccgggagc gatagcgacg gacgcggggg
     9421 cagtacccgc cgcctccaca tattcagcaa tttaaaatat tataagcaaa aaagaaaaat
     9481 tgattgtaaa cataaaaatt ttttaggaga aatttggtat gtttttgaaa aatataaggt
     9541 ttttgatttt ttttatttta ttaacatctt taagcattta ctcactaaat ttagaagaag
     9601 aaataaaaac gagaaaaatc cctacatatt taataaagga tgttcagtat atctcactaa
     9661 aatcattcct taaaataata aattccgata actgggctaa aattgaggat aggattttcg
     9721 tattttttga tggtacacag ataaaattca gaatagatga taaaacttta attgtaggtg
     9781 gtaaaacgat tgagattaaa aatactccaa aagaaattga aggagacata ttaataccag
     9841 ttgaggattt ttcaaaaata tttgcagatt tcaaaatttc agaaaaaaaa gaagaaagag
     9901 aaaaaacatc agaggaaaaa aaagaaagta gaaaatttat tatagtaata gaccccggtc
     9961 atggaggaca tgattcaggt gctattggaa attatggttt aaaagaaaaa gatgtgaatc
    10021 ttgatgtatc cttacgactt gaaaaatatt taaaaaaaca acttgaaagt tatccctcta
    10081 tagaaattta tttaacaaga aaggaagatg tttttatttc acttgaagaa agagttaata
    10141 ttgcaaaaac attaaatgct gatattttct tctcagttca tacaaactct tcaagattta
    10201 atagatttga tgcaacaggt tttgagacat attatccata ctgtaaagta gaaacaaata
    10261 gtttaccaaa atccaatgat ggagaaggag aattaaaaga agaaaataat gattctgtat
    10321 taaatcagat attaaataat ttaaacgaaa ccgatataat tgatgaaggt agaattcttg
    10381 cagaatgtgt tcaggaaaaa cttgcagaaa gattaataac tcctgacaga ggggctaaaa
    10441 gagggaattt ctatgtttta aagtatactc cgatgatttc tgttttaacc gagattggat
    10501 ttatatgtaa tcctaatatt gaattcaatc tgaaagatgt tgaagtaaga caggcaattg
    10561 cagagacact tggaaaagca attattgaat atttaaagaa aagagaaata ataaaatgaa
    10621 taaaaatccg atcgggattt ttgattcagg tgttggtggt ttaagtgttg taaaagaaat
    10681 tataaaagtc ctgccagaag agaatatcat ttattttgga gacacagcaa gggttccata
    10741 tggaaccaaa tcagaaaggg taattaaaaa atttgcagtt gaagatgtta tttttctttt
    10801 aaaatacaat ccaaaattga tagtaattgc ctgccatact gtatcttcat atgcttccta
    10861 ctatttgaga aaaaaattta aaaatatcaa aattattgat gttattgaac caactgttga
    10921 aaaagcagta aaattaacaa aaaataaaaa aattggagtt attggaacaa ccgctacaat
    10981 ttcaagttta agatatcaaa agttattttc aaaatataaa gggatagaag tttatacgaa
    11041 agattgtcct ttatttgttc ctgttgttga agaaggatgg tttgaaaatg aaataagtta
    11101 taagattgct gagatttact taagtgaatt aaagaaaaaa aatattgata cacttgttct
    11161 tggatgtacc cattatcctt atttaaaaaa aacaatacag aaaattatgg gaaaaaatgt
    11221 tgaaataatt gatgcttctt atgaagtttc tatcaaaata aaaaatataa ttgaagaaaa
    11281 taatttaaac aataaaggtg gcaaaggtaa aatatcattg tatttcagtg atataagtcc
    11341 acatgtggaa aaaacaatca aatttctttt tgaaaaaaat aacataaaat ataaacttac
    11401 taaaaatgta tgaactaaaa attaatggag aattttcttc ggctcacagt ttaagaaatt
    11461 ataaaggtag atgcgaaaat ctacacgggc ataactggaa agttgaaatt atagtaacag
    11521 gggaaacact tgatagaatt ggtttactta ttgattttaa tatcttaaag aaaaaactga
    11581 acattatttt aaaaatgctt gaccataaaa atttaaactc aatacccttt tttatagaaa
    11641 gaaatccaag ttctgaaaat attgctttct ttatttataa aaaaatggaa agattactca
    11701 gaggttatcc tgtaagaatt caaaaagtaa cggtatggga aaatgaaaaa caatgcgcaa
    11761 gttactatga ataaaaatta ctggatagtt gaaatattta agaaaagaaa tataagggat
    11821 gtttttggag aggatgttaa aaaaagtatt gaggaattgg gaatttctga tattgaggaa
    11881 gtgaaggttt caaacttata caaaattgag tgtgatacag aaaaaaagaa tatagaaaaa
    11941 atagcaaaag aacttcttat tgataatgtt tctgaagatt ttttaattta taaaaaaagc
    12001 aaaacaaaaa aaaattactg gatagttgaa gtatttttaa aagaaggagt aactgaccct
    12061 gttggtgaaa caagtaaaaa aacaataata gaaagcggaa ttttaaaaga tgtgaatgta
    12121 aaaacaggga aaaaatatta tataaaggga aaactgcaag aaaaacagat aaaagaaata
    12181 tgtaaaaaaa ttcttgcaaa tactttaatt cataattatt ttatttttaa aaaggaaggt
    12241 atgggcaaat gaaacatgca aaagaaattg atattttaaa tgctgacgat aaaaccctaa
    12301 ttgaaataag tgaaaaaaac cttctatcac ttgatatagg tgaaatgaga aaaataaaag
    12361 aatactttct ttcaatagga agaaacccaa cagattgtga acttgaatgt cttgcccaga
    12421 catggagtga acactgctgt cataaaacat ttaaagcaaa tatcaattat gtagaaaaaa
    12481 tcggagaaaa agaagaaaaa attaacatct gtttacttaa agaaataatg gaagttacag
    12541 aaaagttaaa ttatgactgg tgtatttctg tttttaaaga taatgcagga ataattgaat
    12601 taaatgaaaa atggggagtt gctttcaaag tggaaacaca taatcatcca tctgcccttg
    12661 aaccatatgg aggcgcagga acaggaatag gaggagtaat aagagatatt cttggtgtcg
    12721 gtcttggagc aaaaccaatt ttaaacactg atgttttctg tttcggtcct cttgactatt
    12781 catatgaaaa attacctgaa ggcgtcttgc atcccagaag agttttcaga ggagttgtaa
    12841 gtggtgtaag ggattacgga aacagaatgg gtattcctac aggaaatgga gcaataattt
    12901 ttgatgatgg atatgtttat aactgtcttg tttactgcgg aacagtcggg attttacctg
    12961 ttgataaagt ggaaaaaaaa gtaaaagatg gtgatttcat tgttgttatt ggaggaagaa
    13021 ctggaagaga tggtattcat ggagcaacat tttcttctgc aagtttaaaa gaggatatcc
    13081 cgacaagtgt tgttcaaata ggaaatccaa tagttgaaaa aaaatttcag gatgttttac
    13141 ttgttgcacg ggatttgaat ttatataact ccatcaccga ttgtggtgca ggcggatttt
    13201 cttcagcaat tggcgagatg ggaaaggata caggtgcagt tgtttatctt gataaagtac
    13261 ctcttaaata tgaaggttta ctcccgtggg aaatttttct ctcagaatct caggaaagaa
    13321 tggttctcgc agttccagaa aaaaatattg aaaaactcat atctcttttt aactccgaag
    13381 atgttgaagc aactgtgata ggaaaattta taaaaacagg taaattaact gtttattata
    13441 aagatgaaat tgtttgtgat attgatatga aatttcttca taaaggaata cctgaaagaa
    13501 atcttaagtg tttttttgaa aaaaaagaag aaaagaaatt aaatttcaaa tcaaaaaatc
    13561 tgaaagagga tattttaaaa ttaatttcaa cacctgttat atcttcaaaa gaagaagttg
    13621 ttcatcaata tgaccacgag gttggaggaa gaactgtttt aaaaccactt ttaggatatg
    13681 aaagcagggg tgtatctgat ggagttgtat tgaaaccatt ttacgaaaca tatgaaggat
    13741 tagttatagg ttgtggaata aatccttttt atggagaaat tgacccttat tatatggctg
    13801 gatgctgtat tgaagaatgt ataagaaata ttgtaagttg tggaggaaat cctgaaaaaa
    13861 ttgcaatact tgataatttc tgctggggaa atgtttccga tgaaaaagaa cttggtaaac
    13921 ttgtaagatg tgtgaaggga tgtaaggatt atgctttgaa atataaggtc ccttttattt
    13981 caggaaagga tagtttaaat aactatttca ggggaaagaa tggaaaaata atttcaattc
    14041 ccggaactct tcttatctcc ggaataggtg ttattgaaga tgtaagaaag ataacctcaa
    14101 ctgaatttaa aaagggaaga aatattatct atgtcctcgg gaaaacgttt aatgaactcg
    14161 gctcatccca gttttacaga atgtatggta taaaaggtgg aattgtacct aaaccaagac
    14221 cagaaaaaac tttgaattta atgaaaaaaa ttcatacagg tattaaagaa ggactgatta
    14281 aaagttgtca tgactgttca gatggtggat taataacaac tataaatgaa atgatgatag
    14341 ggggaaatct tggatgtgaa ataaattttc ttgatgtttt aagtgaagaa aaagaacctg
    14401 aaataattct tttttcagaa agttgtggta gatttgttgt ggaagtggaa aaagaaaaaa
    14461 taaatgaatt tgaaaaactt tttaaaggcg aagaagtgag tcagatagga caggttatag
    14521 aaaaaaagga aatgattggt ttctggggaa ataaaaaatt attcaaaatt tctcttgacc
    14581 taatttatga aaactggaga agtttaaaat tttaaaaatg aaaccaaaag ttataatttt
    14641 aagagttgca ggtacaaatt gtgattttga aacagagtgg gctttcaatc ttgcaggtgc
    14701 ttctgctgaa agaattcata taaatcagat tataagaaaa gaaaaaaaac tctctgatta
    14761 tcagatactt gttataccgg gtggattttc ttatggagat gatataggtg caggaaaagt
    14821 tcttgcaaat caaataaaat taaaactgtg ggatgaagta gaaaaattta tttttgaaaa
    14881 aaaagtaatt ctcggtattt gtaatgggtt ccaggtcctt gtgaaatccg ggattttacc
    14941 atttaactct gaacaatctg taacacttga atggaatgat tctggaagat ttgaggatag
    15001 atggatttat ttaaaagtag aaaaaagtat ctgtcccttt tttgaaggtc ttccagatat
    15061 aataagaatg cctgttgctc atgctgaagg aaagttctat cctaaaaata gtaaaagttt
    15121 gaaagaaatt gagaaaaata aacaggttgt gttgagatat tgtgataaaa atggaaatct
    15181 ttctggttat ccttataatc caaatggttc tattggaaat attgcgggga tatgcagtaa
    15241 agacggtctt attgtaggaa tgatgccgca tcctgaaagg gctattctta agtattatta
    15301 tcctgatttt ctgaaaaatt atggagatga atttacccct tcctttaaaa ttttcaaaaa
    15361 tatggtaaaa tatttctctt aaaaaattta aaggattata ataataaact ataaaaggag
    15421 caaaagatga cagatgaagt tggaaaaaaa gcagatattc ttgttgaagc atttaaatat
    15481 attaaagaat tcaaaaatag aatatttgtg attaaagttg gtggaagcat ttatcagatt
    15541 gaagatgtta aagaaagtat tctttttgat gttgcatttc tttcttttct tgatataaaa
    15601 attatacttg tatgtggcgg aggaaaattt ataaatgatg agattgagaa gaaaggaggt
    15661 aaagcaaaat ttgttgatgg attgagggtt actaatttac agacattgaa tatagttaaa
    15721 gatgttttat tcagggtaag ggatgaaatt gtaactattt taaaggataa atttgaatgc
    15781 aatgttgata agattactcc tgaagaaaag tttgtcactg cctcaaaaat ccattatcaa
    15841 aagggtgagg aagtcgtaga ccttggattt gttggacaac ttaaaaaagc aaatattgaa
    15901 tatatagaaa ataaacttaa agaaaaaaat atacttgtct gtgcgccact tgcgatggga
    15961 gacgatgaaa atctatacaa tgtaaatgga gatacatttg cttcatttgt tgcctcaaat
    16021 ttgaaaagtg aaaaacttat ttttattacc gatgtactcg gaattatgag aaatcctgaa
    16081 aatccagaca ctttaatttc aacattaaca gtttcgcagg gagaagaatt aataaaagat
    16141 ggtgttataa aagaaggaat gataccaaaa gtaaaagcag ggataaatgg aataaaaaat
    16201 ggagtaaaat cggttcatat tataagtggt aatattcctc attctttaat tcttgaagtt
    16261 ttaacagaac acggagttgg ttcagagata ataaaaaatg gataaagaaa aaattttaaa
    16321 aaattataat gattatgtta tcccgtctta taggaagcag aattttattt ttgttaaggg
    16381 gaaagggagt tatttatggg acattgatgg aaaaaaatat cttgattttt tcccgggatg
    16441 ggcagtaagt ggtcttggac actgtcatcc acaggttgta aaaaaaatta aagaacaggt
    16501 tgaaatactg atacatattt caaataatta ttatcatcca tggcaggcac ttcttgcaga
    16561 aaaactctcc agattatcat ttggtggtaa agtttttttc tgtaacagtg gagcagaagc
    16621 aaatgaatct gccattaaac ttacaaggtt gtatggaagg aaaaaaggaa aatataaaat
    16681 tatttcaatg gaaaattctt ttcatggaag aactcttgca acagttacaa tgacaggaca
    16741 gaaaaaatat aatgaaatgt ttgagccact tcccccgggt tttatttatg caaaatttaa
    16801 tgatttttca gacctttatt caaaaataga tgatgaggtt gcaggaataa tacttgagtt
    16861 aatacaggga gaaggtggaa ttaatgtagt ggataaagat tacttaaaaa aagtcaaaga
    16921 tttatgtgaa aaaaaagata ttcttttaat ttttgatgaa gttcagaccg gatttggtag
    16981 aacaggagaa ctctttgcat atcagatatt tggtgttgaa ccggatatca tgactcttgc
    17041 aaagacactt ggaggaggtt ttcctgttgg agctatgatt gccagaaaag aaattgcaga
    17101 tttaatggga ccgggaaccc atgcatcaac ctttggtggt tctcctcttg cctgtgctgc
    17161 ctgtcttggt gttcttgaag caatagaaaa tgaaaacctg cttgaaaatg taaaaattat
    17221 gggaaaatac cttaaagaaa aacttgaaaa attaaaagaa gaattcaaag ggataaaaaa
    17281 agtcaaagga ttgggactaa tgcttgcaat tgaaattgaa aaacagggtg aggaactcgt
    17341 aaatatatgt gctaaaaacg gacttcttat aaactgtacc catcagaatg taataagaat
    17401 aatgcctgct ataaatgtta aaaaagaaga gattgatgaa ggaatagaga tattaaaaaa
    17461 atcattaaaa gaggcaggaa ttcaatgaaa agagatttcc ttaagataaa agatatttca
    17521 aaagaagaag tatataaaat ctttgatatt acaaaatatt ttaaaagttt aaaaaaagaa
    17581 aaaaaaatta cagaacacct caaaaatctg acaattggat tattattttc aaaaccaagt
    17641 acgagaacca gaatatcctt tgaagtcgca attcaacaac ttggcggttt tccaatttat
    17701 ctttcatccg aagtaactca aatttcaaga ggagaatcaa ttaaagatac agcaaaagta
    17761 atttcacgat atttagatgg aatcataata aggacttatg cacaggaaga agt
//
LOCUS       DAOSAT010000028        17232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2287_length_17232_cov_5.160214, whole genome shotgun sequence.
ACCESSION   DAOSAT010000028 DAOSAT010000000
VERSION     DAOSAT010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17232
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2287_length_17232_cov_5.160214"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1330)
                     /locus_tag="PLF90_03685"
     CDS             complement(104..1330)
                     /locus_tag="PLF90_03685"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF017903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOK56419.1"
                     /translation="MNYEKWIENGIKQLEEEFGEKKVKKAIEYVKNFEVEIPSWIFGN
                     FGGGRFGNYTPPGFARNIYEKIDDASLVHKLTGCTPRIATHILWDFSNDGIEPDEKIV
                     KDVYEYTKEKGISLGAINPTYFLTGSYRGSFTSPDKKTRERYIEQTIFGADISKKYGN
                     KLLTLWFPDGSLYPGQINLKENYELMKKTLIESYEKIPEDVKVLVEYKLFEPGTYSTT
                     IPDWGTSYILTRALGKNAGVLIDLGHHPHGTNIEQIVAILISENISCGFHFNTRYAAD
                     DDHAVEPNLQMARIFYELVKGKVICNKEKIKNWAYMIDQASGRENRFHSILHSIDSLQ
                     ISLARGVLVDEEKLKEFQDKDEIILANRTFNSATSLCDVRPIVFKARLEKNIPIDPLK
                     SYIESGYQKKIEEERK"
     gene            complement(1355..1702)
                     /gene="rnpB"
                     /locus_tag="PLF90_03690"
     ncRNA           complement(1355..1702)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLF90_03690"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1703..2110)
                     /locus_tag="PLF90_03695"
     CDS             complement(1703..2110)
                     /locus_tag="PLF90_03695"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOK56420.1"
                     /translation="MEKILIYIDGASKGNPGKSAYGIVIFKNEKIYKKIGDYIGITTN
                     NIAEYLSLIFALIECLPFRTDEIEVRSDSQLLVKQITGEYKVRDEKLLIFHKIATFLF
                     SKYNNIKIIHINREENKIADELANSFIENNGLF"
     gene            complement(2110..2811)
                     /locus_tag="PLF90_03700"
     CDS             complement(2110..2811)
                     /locus_tag="PLF90_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOK56421.1"
                     /translation="MINEEIEKLLILQEIDNEIFEKEKEINAFPEKEKKLNKEIEDIE
                     KQIKTLKDDLKKLQLDRKEKELEVRSYEEELKNLNKKLDEVKSNKEYEALLIEITNIK
                     KKISDIEEDILILMEKEDDILKKEKSLEEEIKKTKEKISKLIEEERKKIENLKIQVEE
                     KKKERENLVKEIEYKIYSLYEKIRKSKKDKIAVVKIENGICGGCYMTLPTFIVEKVKK
                     KEEIIQCENCSRILF"
     gene            complement(2853..3872)
                     /gene="rfaE1"
                     /locus_tag="PLF90_03705"
     CDS             complement(2853..3872)
                     /gene="rfaE1"
                     /locus_tag="PLF90_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOK56422.1"
                     /translation="MKFSLEKIINLEKIEKNFKKFTLKKILIIGDIILDHYIFGEVER
                     ISPEAPVPVVELKNEKFLLGGAGNLALNIKSMGGEIFLISTTGKDKNGKILKDILKKE
                     KINSFILQRDVPTIVKTRVIAKNQQIVRIDREKIEKLNKKEEEKIKDVILKEIESTEI
                     IVISDYGKGIITEDIINFLKKFKKRIIVDPKPEHFNFYKNVFCLTPNKNEACLGIGKI
                     NVKELKDIIQTGIEIIKLLKCKNLIITLGKDGMLIFKNKEEIYKIPSIAKDVYDVTGA
                     GDTVCGIFSLYLSVSNDILESAIISNFAAGIVVGKIGTATTNKKEFIEFFKKSFKDIF
                     IEKIK"
     gene            complement(3876..5804)
                     /locus_tag="PLF90_03710"
     CDS             complement(3876..5804)
                     /locus_tag="PLF90_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56423.1"
                     /translation="MNTFFTKRSFITGILFSILIAFVDHYSTDVIHASYMAIDHMPAG
                     AIFIFFILVFIINTFLKLIQKKIKKNIAFNPGELLVVYSMMLVASSVTEMGLGSQILP
                     IISAPTYYSSPQNEWEKLIIPYLKKVLFVTDKEAVKYFYEGLPKGMAIPWKIWILPLI
                     FWLPFIFTLYFVMICIASILRKQWAEREKLVYPLTILPKEMTEEGKGILPNFFKNPLM
                     WFGFSIAFIIGTSVALNFYFPAFPKIQLVKGIPIFRRTLTLNFRVSFPVMGFIYFINL
                     DVAFSLWFFNLVFKVIRGMFNIAGIVSTENVGIYGCSGEAIFAHLGMGAMIMMIGYSL
                     FLWRHHLKDVFAGAIGKKVDDKDEILSYKSAFWGIVFGSLFMILWLFFSGMKWYIAVL
                     FLIFAFVIWLTLTRIVCEGGIPTMVATTIASAQIISMFGSKNLTPFTIIALGLTYIYA
                     ADLRTFPLASTSMSLKMIEKSESNRRPFFWFLFLAIFVNIITTLILELKLAYKYGGIN
                     LNNWYFVGGPQAPYKYAADIIKNPTLPNKIGWLCRGIGFAVMGILIFARQNFLWWPLH
                     PIGFLIGPVWLMDQLWFSIFVVWLIKKLILKYGGAQIYNKFKYFFLGLPLGLYTCAGV
                     WFIIDMITGKQGNVIFWI"
     gene            complement(5801..7252)
                     /gene="gatA"
                     /locus_tag="PLF90_03715"
     CDS             complement(5801..7252)
                     /gene="gatA"
                     /locus_tag="PLF90_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOK56424.1"
                     /translation="MKSEILSFSVCELKKLYKKKEVKPSEVVSLLLEEIYRKDKDIHS
                     YLYVTEKTIEEAKRIDEKIAKGEELKELEGIPVSIKDNICTKGMPTTCASKILENFIP
                     PYDATVIERLKNAGAIIIGKTNMDEFAFGSSTENSAFGPTKNPFDLERVPGGSSGGST
                     ASVASNLCFASLGSDTGGSIRQPSAFCGVVGMKPTYGRVSRYGLIAFASSLDQIGPIT
                     KNVKDNALILKVISGKDKFDSTSVEIMVPDYINFLEKDLKNIKIGVPKEYFTEGIEKE
                     VEEKILKVLKILENTGAKIEEISLPHTSYGIATYYIIATAEASSNLARFDGVKYGYRT
                     QDYKNLMEMYMKTRGEGFGEEVKRRIILGTYVLSSGYYEAYYLKAQKVRTLIKNDFEE
                     AFKKVDVIVTPTTPELPFKIGEKKDEPLKMYLSDIFTVNINLAGLPAISIPCGFSYNN
                     LPVGVQFISPYFKEENILNVSYIVENEIKGGKI"
     gene            complement(7249..8502)
                     /locus_tag="PLF90_03720"
     CDS             complement(7249..8502)
                     /locus_tag="PLF90_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOK56425.1"
                     /translation="MGKTIVEKILSSHSGKDARSGEIVLAKVDFLMAQDGTAPLVIKS
                     FENLGINEVFDPKKSVFVIDHNSPSPNEGVSSLHKLMREFAKKYGIEIFEVGEGVCHV
                     LLPEKGKVKPGDLVLGADSHTPTYGALNCFASGVGSTDLAIALVTGSLWFKVPETIKI
                     EITGKIPFGIFGKDIALYIVKNLKAEGAIYKSVEIKGEVIKNLSMDERFTLCNLTTEI
                     GAKCGIMEFDEKTKEYMEKRRIKEYTPVFPDKDATYESIFNFDISNISPQIAKPHQVD
                     NVCNVEEVEGTEINQGFIGTCTNGRVSDFEIAAKILKGKKIKKGIRLICTPGSREIYL
                     ECLKKGYIEILIESGACVTNPGCGPCVGTHQGIPSDGEVVISTANRNFKGRMGNPNAF
                     IYLASPATVAASCIEGKITDPRKYL"
     gene            complement(8505..9734)
                     /locus_tag="PLF90_03725"
     CDS             complement(8505..9734)
                     /locus_tag="PLF90_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HOK56426.1"
                     /translation="MNKIYFIDVTNRDGVQTAHLGLAKLEKTMINIYLNQMGIFQSEA
                     GFPVTKHEINYLNANMNLVEKKVLHPIRISGWLRAITSDVEEAFKNIPKLKHVNLSIS
                     TSEQMIMGKFQGKMGFGEVIKAMVEAVKAAKALGAETIGVNAEDASRTKIENLIEFAL
                     AAKEAGADRIRYCDTLGYDTPFTIYENIYKLAESTKMPIELHCHNDIGMAVACSIAGA
                     KAAIDAGVDAYINTTINGMGERAGNADLVSCILAIKYGAGMNKYKLDENINLKMAWKI
                     THYASYAFKVPIPINQVGVGKNAFTHSSGIHVDGALKDRRNYELYDFEELGRGEPEIV
                     ETGRMIILGEYSGIKGLRNIYGKLEIEFKDDDEARKILELARYANVHKQKPVTEDELI
                     FIAKYPEEARLLMTMTP"
     gene            complement(9731..10180)
                     /gene="rplM"
                     /locus_tag="PLF90_03730"
     CDS             complement(9731..10180)
                     /gene="rplM"
                     /locus_tag="PLF90_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOK56427.1"
                     /translation="MTKTQVPKKERIKRKWYIVDADGQILGRLASKVAKILMGKHKPI
                     YTPNVDTGDFVIVINAEKIKVTGKKLTDKIYYKHITGYLGHLKEEKLISLLNRRPEAV
                     IKLAVEGMLPKNSLGRKMLKKLKVYRGTEHPHKAQKAEVLNLKEVKI"
     gene            10332..10931
                     /locus_tag="PLF90_03735"
     CDS             10332..10931
                     /locus_tag="PLF90_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019999608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOK56428.1"
                     /translation="MIEILIFLVILFFSIIVHEVSHGYAALKNGDPTAKYMGRLTLNP
                     IPHIDPVGTLLLPFLLIVLHFPILIGVAKPVPINPYYFRNYRTGMITVGISGPLSNIL
                     LGIIFAFIYKLFPSSIVSGYLKMGSFINFILAFFNLIPIPPLDGSRVVAVLLPYKLRF
                     GYEKIERYGLFLVLLLAMTGFLNWLSPLSQSLTNFFINL"
     gene            10978..11250
                     /locus_tag="PLF90_03740"
     CDS             10978..11250
                     /locus_tag="PLF90_03740"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOK56429.1"
                     /translation="MKFNIIVRFFAEMGWQALGKISNPLTGKTEKNLEIAKQVIELLE
                     TLKEKTKGNLTEEEDKFLNSAIADLQLNYVEEVSKGKKENESENKT"
     gene            11228..12676
                     /locus_tag="PLF90_03745"
     CDS             11228..12676
                     /locus_tag="PLF90_03745"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOK56430.1"
                     /translation="MNQKIKLKLKTLPDSPGVYLMKDKEGNVIYVGKASSLLKRVRSY
                     FSPSGNNTKILALAEKIYDFEVIPVISEAEGLILENQLIKKYKPKYNTNLKDDKSYPL
                     LKITKEEFPSVQIVREKKTDKAIYFGPFTNKKLLKETVRFLRKFYPVRNCKKNVSTGK
                     VKLCIQYHIGRCSGPCENKIKKEEYQKLVEGIIAFFEGKYKEFEKKLKKWMLEEIKKL
                     NFEEADKIKKRLFLLNEMAEKFPVREEEELYRYSEENILFNLSKILKLSKIPNLIEGY
                     DISNISGDFAVGSKVSFIAGIPYKDGYRRYKIKTVEGIDDYKMLEEVLIRRFDTEEEK
                     KQIPDLILVDGGKGQLGVAVSVLKRYNLKIPVISLAKREEKIFVEWSEEGIILPISSP
                     ELQLLQRIRDEAHRFAISYHRKIRSKNFKFSFLDEIKGIGEKTKKRIIMNFPDVNLIA
                     NLNVVQLKQIGINEKIAEKIIEKAREVISNEK"
     gene            12666..12926
                     /locus_tag="PLF90_03750"
     CDS             12666..12926
                     /locus_tag="PLF90_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56431.1"
                     /translation="MKNEKIFEKFLIFGIGSIYFIGENLKRIMKEIEKEGNEHAESIE
                     KIKKEILKFIKMPKNLIVEFLKSCDFITKEDLEKFKEDIKNG"
     gene            12919..13908
                     /gene="obgE"
                     /locus_tag="PLF90_03755"
     CDS             12919..13908
                     /gene="obgE"
                     /locus_tag="PLF90_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOK56432.1"
                     /translation="MVKFIDEVQIFVKSGKGGDGCIAFRRERCIPKGGPWGGDGGKGG
                     DVIFKAVKNKKTLIDFHFQKHFYAENGRPGSSNNKKGKDGEDLIIEVPCGTIVKEVIN
                     GEETLLSDLVNEGDTVIVAKGGKGGLGNTHFKSSTNQAPRIATKGEPGEEKVIKLELK
                     LIADVGIIGYPNSGKSTLLSKISKAKPKIADYPFTTLEPNLGLVDLGDFRSFVVADIP
                     GLIEGASKGKGLGIKFLKHIERTKILIHLLDLSENGIINRYQNIRKELKNYSDKLVEK
                     KEIVVGNKIDLEESKKNIKLATDYFKEIYFISALKGEGIKELLERIWKELKND"
     gene            13901..14983
                     /gene="prfB"
                     /locus_tag="PLF90_03760"
     CDS             13901..14983
                     /gene="prfB"
                     /locus_tag="PLF90_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOK56433.1"
                     /translation="MIKLKERIEDIKKYWEEIKEKFKIKEDLKTFELLEKEMHEKGFL
                     DDKNKIELWTSLKEKKELVDEIEKDIKESETLFELIEESTDEELEREGEEILKRLEEN
                     IKKIKFEYIWKEEDVNNAIITLHSGTGGTEACDWVSMLWRMYSKWASKKGYDVKVIDF
                     LPGEEAGLKRITAIISGKNAYGYLKGETGVHRLVRISPFDANKRRHTSFAGVSVIPEV
                     KEDIKIEIKDEDIEIETFRAGGHGGQNVNKVETAVRIKHKPTGIVVQCQNERSQFKNR
                     EIAMKILKSRLYQLYLEEKRKSQEEKRSQEKEITWGSQIRSYIFCPYTLVKDHRTEVE
                     TSNIEGVMDGDIDEFLEAEIEWLAKR"
     gene            14965..15588
                     /locus_tag="PLF90_03765"
     CDS             14965..15588
                     /locus_tag="PLF90_03765"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOK56434.1"
                     /translation="MAGKKIKVLISAGPTREYIDPVRFISNPSTGKMGYLIAEYAKKK
                     GFDVILVSGPTYLKPPEGVKLIKVETAEEMQKEILKNFPKVDLLIMAAAVSDWRPFRK
                     FKNKLKRKNQWTLKLIPNPDILKSVAKIKKESQKVVGFALETEDIIKNAEKKLKEKKL
                     DLIIGNTPDFFGKGKKSDVWFIFKDGKVINFKIKKEQLPPKIFEVFF"
     gene            15640..16746
                     /locus_tag="PLF90_03770"
     CDS             15640..16746
                     /locus_tag="PLF90_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK56435.1"
                     /translation="MNRKVKIGIIGVGQIGKSHIKKYEVIPDAEIVAVCDVNEEEAKR
                     VCETHNIPKYYTDFRELLKREDIEAVDVCLHNNLHMLVTVEALNSGKHVYCEKPMAGS
                     YADALKMYETSKKTKKYLSIQLNTLFSKETKAAKILIEKGYLGKIYYGRSTGYRRLGR
                     PFVDGYGSKFFVQKEVAAGGALYDMGVYHIANLLYLMGNPEVLRISGKTYQEVEINEK
                     RKEISNCNVEELGIGFVKFKNNIVMEIVEAWAIHLDPFESSYIVGNKGGIRLNPLRYF
                     TNIEGDMPMDATFNLDILDYRWHNVYENGDVYDSPQHHWIAVLQGRVELLPTAEIALN
                     TMLISEGIYLSDVLGREVTAEEVKQNSKSTALKI"
     gene            16762..>17232
                     /locus_tag="PLF90_03775"
     CDS             16762..>17232
                     /locus_tag="PLF90_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOK56436.1"
                     /translation="MKVKIAVQLFSVRNDCAKDFYGTLKEVAKIGYEGVEFAGYYEKS
                     AEEIKKMLDDFNLKVAGTHIRIDTLLGDELKKTIEFNRIIGNKYLIVPILPEEMRNSK
                     EKWLEAAKLFNKISDEVRKEGMYVGYHNHAVEFQKIDGEYPWDIFFKNTVPDVVM"
BASE COUNT         5945 a         2586 c         2595 g         6106 t
ORIGIN      
        1 actcctatat tcaccaaaat ataccatttt tcaccttttt tataaaattt aatacccact
       61 tttaattttt gtcaaatttt tttgttttat aaaaaaggtg gttttatttt ctttcttctt
      121 ctattttttt ctgatatcca ctttcaatat aactttttaa ggggtcaatt ggaatatttt
      181 tttccaacct tgctttaaaa acaataggtc ttacatcgca caaagaagtt gcagaattaa
      241 aagttctatt agcaagaatt atttcatcct tatcttgaaa ttcttttaat ttttcttcat
      301 caacaagaac acctcttgcg agtgaaattt gcagtgaatc tattgaatgt aaaattgaat
      361 gaaatctatt ttctcttcca cttgcctggt caatcatata agcccagttt tttatctttt
      421 ctttattaca tataacttta cccttaacaa gttcataaaa tattcttgcc atctgtagat
      481 tgggttcaac tgcatggtca tcgtctgccg catatcttgt attaaaatga aaaccgcatg
      541 agatattttc tgaaattaaa atagcaacta tttgttctat atttgttcca tgtggatgat
      601 gtccaaggtc aatcaaaact cctgcatttt tcccaagtgc tcgggtaagg atataggatg
      661 ttccccagtc aggaattgtt gtactgtaag ttcctggttc aaataattta tattcaacaa
      721 gaacttttac atcctctgga attttttcat aactttcaat tagagttttt ttcattaatt
      781 cataattttc tttcaaatta atctgtcccg gataaagaga accatctggg aaccataatg
      841 ttaaaagttt atttccatat tttttggaaa tatcagcccc aaaaatagtt tgttcaatat
      901 atctttctct tgttttttta tcaggtgagg taaaacttcc tctataagag ccagtaagaa
      961 aatatgtggg atttatggct ccaagagaaa ttcctttttc ttttgtatat tcataaacat
     1021 ctttaacaat tttttcatca ggttcaattc catcatttga gaaatcccag agaatatgtg
     1081 ttgctattct gggggtgcat ccagttagtt tgtgaaccaa tgaagcatca tcaatttttt
     1141 catatatgtt tcttgcaaat cctggagggg tataatttcc aaatcttcca ccaccaaaat
     1201 ttccaaatat ccaacttggt atttcaactt caaaattctt tacatattca attgcttttt
     1261 ttaccttttt ttctccaaat tcttcttcaa gttgttttat tccattttca atccattttt
     1321 cgtaattcat atatgcctcc tattttaaaa tttaacgaag gagtgaccta taagccggat
     1381 tctgttttag gtgtccattc atcttggtca gcagttacct gctgactctg tgcggtctac
     1441 ccgaaccttt tggggcaggg aacccatagg ttctgcttga ccttgctccg ggtggggttt
     1501 gccatactcc actataccac atagtggacg gtgagctctt acctcgcctt ttcaccctta
     1561 ttcaccggaa tatggtgaac ggtatgtttt ctgtggcact ttccaggtat cgctacctgt
     1621 tcctgttagg aaccaccctc cctgcaggag tccggacttt cctcccacca aaattttaat
     1681 gggcggacac ccggtcactc cttcaaaata aaccattatt ttcaatgaac gaatttgcaa
     1741 gttcatcagc aattttattt tcttctctgt taatatggat aatctttata ttattatact
     1801 ttgaaaataa gaaagttgct atcttatgaa aaattaataa tttttcatcc cttactttat
     1861 attctcctgt aatttgtttg acaagaagtt ggctatcact tcttacttca atttcatctg
     1921 ttctaaatgg tagacattct attagagcaa aaattagaga tagatattct gctatgttat
     1981 ttgttgtgat tccaatataa tctccaattt ttttatatat tttttcattt ttaaaaatca
     2041 ctattccgta agcactttta ccaggatttc cctttgatgc tccatcaata tagataagaa
     2101 ttttttccat caaaataaaa ttctactgca attttcacac tgaataattt cttctttctt
     2161 ttttactttt tccactataa aggtaggaag ggtcatataa catccaccgc atatcccatt
     2221 ttcaattttt accactgcta ttttgtcctt tttagatttc cttatttttt catataaact
     2281 gtaaatttta tattcaattt ctttaacaag attttctctt tctttctttt tctcctcaac
     2341 ctgtatttta agattttcta ttttttttct ttcttcttct attaactttg aaattttttc
     2401 ctttgttttt ttaatttctt cctctaaact tttttctttt ttcaatatat catcttcttt
     2461 ttccattaaa atgagaatat cttcttcaat atcagaaatt tttttcttaa tatttgttat
     2521 ttcaatcaga agggcttcat attctttatt tgatttcact tcatcaagtt ttttatttag
     2581 attttttaac tcttcttcat aacttctgac ttcaagttct ttttcttttc tgtcaagttg
     2641 caactttttt aaatcatctt ttaaagtttt tatctgtttt tcaatatctt caatttcttt
     2701 gtttaatttt ttctcttttt caggaaatgc atttatctct ttttcttttt caaatatttc
     2761 attgtcaatt tcctgaagaa tcaaaagttt ttctatttct tcatttatca ttttttcctt
     2821 tccagttgtc aatataaata attataaact ttttatttta ttttttcaat aaaaatatct
     2881 ttaaaggatt ttttaaaaaa ctcaataaac tcttttttgt ttgttgttgc tgttccgatt
     2941 tttccaacaa caattccagc agcaaagttt gaaattatag cactttctaa aatatcattg
     3001 cttacagata gataaagtga aaatattccg caaacagtat ctcctgctcc tgtcacatcg
     3061 taaacatctt ttgcaataga aggaatttta tatatttcct ctttattttt aaatatgagc
     3121 ataccatcct ttccaagagt tattattaaa tttttacatt ttagtaattt tattatttca
     3181 attcctgttt gaatgatatc ttttaattct ttaacattaa tttttcctat tccaaggcat
     3241 gcttcatttt tattgggagt aagacaaaaa acatttttat agaaattaaa gtgttctggc
     3301 tttggatcaa caattattct ttttttaaat ttttttaaaa aattgataat atcctcagtg
     3361 ataattcctt taccataatc tgaaataaca ataatttctg tactttcaat ttctttcaaa
     3421 ataacatctt ttattttttc ctcttctttt ttatttaatt tttcaatttt ttctctatca
     3481 attctcacaa tttgttgatt ttttgcaatt actcttgttt taacaatagt ggggacatcc
     3541 ctttgaagaa taaatgaatt gattttttct tttttcagta tatctttcaa aatttttcca
     3601 tttttatcct ttccagttgt tgaaattaag aaaatttccc cccccatact ctttatattt
     3661 aaagcaagat tccctgcgcc accgagtaag aatttttcat tttttaattc tactaccgga
     3721 acaggtgctt ctggagagat cctttctact tctccaaaaa tatagtggtc aagaataatg
     3781 tctccaatta ttaaaatttt ttttaaagtg aactttttaa aatttttttc aattttttca
     3841 agatttataa tcttttctaa actgaatttc atctatcata tccagaaaat tacatttccc
     3901 tgttttcctg taatcatatc aataataaac cagacacctg cacatgtata aagtcccaga
     3961 ggcagtccaa gaaagaaata tttaaactta ttataaattt gagcccctcc atattttaaa
     4021 atcaattttt taattagcca gacaacaaat attgaaaacc ataactggtc cataagccat
     4081 acaggaccta ttaaaaatcc aattggatgt aaaggccacc agaggaaatt ctgtcttgca
     4141 aaaatcaaaa tacccattac agcaaatcct attcctctgc ataaccatcc aattttattt
     4201 ggtaaggttg gattttttat aatatcagca gcatatttat aaggtgcctg aggtccgcct
     4261 acaaaatacc agttatttaa attaattcca ccatatttgt aagcaagttt taattcaagt
     4321 attaaggttg taattatatt tacaaaaatt gctaaaaata aaaaccagaa aaacggtctt
     4381 ctattagatt ctgatttttc tatcatttta agcgacattg aggtagatgc aagagggaat
     4441 gttcttaaat cagcagcata tatgtatgtt aatcccagag caataatagt aaatggagtt
     4501 aaatttttgg aaccgaacat tgagataatt tgagcagaag caattgttgt tgctaccatt
     4561 gttggaattc cgccttcaca tacaattctc gttaaagtta accagattac aaaagcaaaa
     4621 attaaaaaca aaactgcgat ataccacttc attccagaaa aaaataacca taaaatcata
     4681 aataaagaac caaatacaat cccccagaaa gcacttttat aagatagaat ttcgtcttta
     4741 tcatcaactt ttttacctat tgcaccagca aaaacatcct ttaaatgatg tctccagaga
     4801 aacaaactat agccaatcat cataatcatt gcacccattc caaggtgtgc aaaaattgct
     4861 tctcctgaac atccataaat cccaacattt tcagtactta caattcctgc gatattaaac
     4921 atccctctta taactttaaa aactaaattg aaaaaccaga gagagaaagc aacatcaaga
     4981 ttgataaaat aaataaagcc cataacagga aaactaactc tgaaattgag agtaagagtt
     5041 cttctgaata taggtattcc ttttacaagt tgaattttag gaaatgcagg gaaatagaaa
     5101 tttaaggcaa cagatgttcc tattataaag gcgattgaaa aaccaaacca cattaatggg
     5161 ttcttaaaaa aattaggtaa aattcctttt ccttcttcgg tcatttcttt tggtaaaatt
     5221 gtaagaggat aaacaagttt ttctctttct gcccactgtt ttctcaaaat agaagcgata
     5281 catatcataa caaaataaag tgtaaaaata aaagggagcc agaaaattaa tggtaaaatc
     5341 catattttcc agggtattgc cattcctttt gggagacctt cataaaaata tttcactgcc
     5401 tctttatctg taacaaagag aacttttttc aaataaggaa ttataagttt ttcccattca
     5461 ttctggggac ttgaataata tgttggtgca gaaattattg gtaaaatttg acttcccaat
     5521 cccatttcag ttacagaaga agcaacaagc atcattgaat atactacaag taattcacct
     5581 ggattaaaag caatattttt ctttatcttt ttctgtatta attttagaaa agtgtttatt
     5641 atgaaaacta aaataaaaaa tatgaaaatt gcacctgcag gcatgtggtc aattgccata
     5701 tatgaagcat gaattacatc tgtggaataa tggtcaacaa aagcaattaa aattgagaat
     5761 aaaatacctg ttataaaact tctttttgtg aaaaaagtgt tcatattttc ccccctttta
     5821 tttcattttc aactatataa gaaacattta aaatattttc ttctttaaaa taaggactta
     5881 taaattgtac accaacaggt aaattattat atgaaaaacc acaaggtata cttattgctg
     5941 gaagtcctgc aaggtttata ttcactgtga aaatgtctga aagatacatt tttaatggtt
     6001 cgtctttttt ttcaccaatt ttaaaaggaa gttcaggagt tgttggtgtt actattacat
     6061 caactttttt aaatgcttct tcaaagtcat tttttatcaa tgttcttact ttctgtgctt
     6121 ttaaataata tgcttcataa taacctgaag ataaaacata agtaccgagt ataattcttc
     6181 ttttaacctc ttctccaaat ccttcacccc ttgttttcat atacatttcc attaaatttt
     6241 tataatcctg ggttctatat ccatatttta caccgtcaaa tcttgcaaga tttgaactgg
     6301 cttctgctgt tgcaattatg tagtatgtag caattccata agaagtatga ggtaaagaaa
     6361 tttcttcaat ttttgctcct gtattttcca gaatttttaa aacttttaaa attttttcct
     6421 ctacttcttt ctctatccct tctgtaaaat attcctttgg tacacctatt tttatatttt
     6481 ttaaatcttt ctcaagaaaa tttatataat caggtaccat aatttcaaca cttgtagaat
     6541 caaatttgtc tttaccactt ataactttta aaataagtgc attatctttt acattttttg
     6601 ttattggtcc tatctggtca agagaagaag caaaagcaat aagtccatat cttgatactc
     6661 ttccataagt tggtttcatc cccacaactc cacaaaaagc agatggctgt ctaattgaac
     6721 cacctgtatc tgaaccgagg gaagcgaaac atagattgga agcaactgaa gcagtagaac
     6781 cacctgaaga gccaccaggt actctttcta aatcaaaagg attttttgtt ggaccaaatg
     6841 ctgaattttc agttgaagaa ccaaaggcaa attcatccat atttgttttt ccaattatta
     6901 tagcacccgc atttttcaat ctttcaataa cagttgcatc ataaggaggt atgaaatttt
     6961 caagaatctt tgaagcacat gttgttggca ttccttttgt acagatatta tcctttattg
     7021 aaaccggtat cccttcaagt tcttttaatt cctctccctt tgctatcttt tcgtcaatcc
     7081 ttttcgcttc ttcaattgtt ttttcggtta cataaaggta ggaatgaata tctttgtctt
     7141 ttctgtaaat ttcttcaagc aataatgaca ctacttctga tggtttgacc tctttttttt
     7201 tatatagttt ctttaattca caaacactga aactcaaaat ctcgcttttc ataaatactt
     7261 tcttgggtct gttatttttc cttctataca acttgctgca acagttgcag gagaagcaag
     7321 ataaatgaag gcatttggat tacccattct tcctttaaaa ttcctgtttg ctgttgatat
     7381 tacaacctct ccatcagatg gtattccctg atgggttcca acacaagggc cacaaccagg
     7441 atttgtaaca catgcaccac tttcaatcaa aatttcaatg tatccttttt ttaaacattc
     7501 aagataaatt tctcttgagc ctggagtaca tatcaatcta attccttttt ttattttctt
     7561 tccttttaaa atttttgcag caatttcaaa atcactcact ctaccatttg tgcatgtacc
     7621 tataaaaccc tgatttattt ctgttccttc aacttcttca acattgcata cattatcaac
     7681 ctgatgtggt tttgctattt gaggtgatat atttgaaata tcaaaattaa aaatactttc
     7741 ataagtggca tctttatcag gaaaaacagg agtatattct tttattcttc ttttttccat
     7801 gtattctttt gttttttcgt caaattccat aatcccacat tttgctccta tttcagttgt
     7861 taaattacat agtgtaaatc tttcatccat agataaattt tttattactt ctccttttat
     7921 ttcaactgat ttataaattg ctccttctgc ttttaaattt tttactatat ataatgcaat
     7981 atctttccca aaaattccga aaggaatttt ccctgttatt tctatcttta ttgtttcagg
     8041 tactttaaac cataaagagc cggttacaag tgctattgca aggtctgttg aaccaactcc
     8101 acttgcaaag cagtttaaag caccataagt tggagtgtga ctgtctgcac ctaaaacaag
     8161 gtctccaggt tttacctttc ctttttctgg aagaagaaca tgacatacac cttctccaac
     8221 ttcaaagatt tctataccat atttctttgc aaattctctc atcaatttat gtaaagagga
     8281 aaccccttca tttggacttg gactgttatg gtcaattaca aatacagatt ttttcgggtc
     8341 aaaaacctca tttattccaa gattttcaaa acttttaatt accaaaggcg cagttccatc
     8401 ctgagccatt aaaaaatcaa ctttagcaag aactatttct ccacttcttg catcttttcc
     8461 tgaatgagag gataagattt tctcaactat tgtttttccc atttttaagg tgtcattgtc
     8521 attaaaagac gtgcttcttc aggatatttt gcaataaata tcaattcatc ttcagtaact
     8581 ggtttctgtt tatgaacatt tgcatatctt gcaagttcaa gaatttttct tgcttcatca
     8641 tcatccttaa attcaatttc aagtttacca tatatatttc ttaaaccctt aattccacta
     8701 tattctccaa gtataatcat ccttcctgtc tcaactattt ctggctctcc tcttccaagt
     8761 tcttcaaaat cataaagttc ataatttctt ctatccttta aagctccatc aacatgaata
     8821 cctgaagaat gagtaaatgc atttttacca acaccaacct gatttattgg aattgggact
     8881 ttgaatgcat atgaagcata atgggtaatt ttccatgcca tttttaaatt tatattttca
     8941 tcaagtttat atttattcat acctgctcca tatttaattg cgagaataca agaaacaagg
     9001 tcagcatttc ctgctctttc gcccattcca ttaattgttg tgtttatata tgcatcaact
     9061 cctgcatcaa tcgctgcctt tgctcctgca atagaacaag caacagccat tccaatgtca
     9121 ttatgacagt gaagttcaat aggcattttt gtactttcag caagtttgta aatattttcg
     9181 tatattgtaa aaggagtatc atatccaagt gtatcacaat atcttattct atccgcacct
     9241 gcttcttttg ctgcaagtgc aaattctatt aaattttcta tttttgtcct tgaagcatct
     9301 tctgcgttaa ctccaattgt ctctgctcca agtgcttttg ctgcttttac tgcttcaacc
     9361 attgctttta ttacttctcc aaaacccatt tttccttgaa atttgcccat tatcatctgt
     9421 tcagatgtgg aaattgagag atttacatgt tttaatttcg gtatattttt aaatgcttct
     9481 tctacatcac ttgtaatcgc tcttaaccaa cctgaaattc ttataggatg taaaactttt
     9541 ttttccacaa gattcatatt tgcgttaagg taatttatct catgctttgt tacaggaaaa
     9601 cctgcttcac tctgaaatat tcccatctga tttaaataaa tattaatcat cgtcttctca
     9661 agttttgcaa gcccaagatg tgctgtttga acaccatccc tatttgtaac atctataaaa
     9721 tatattttat tcatattttc acctccttaa gatttaaaac ttccgccttc tgtgctttat
     9781 gtggatgttc tgttcctcta taaactttca acttttttaa catttttcta ccaagagaat
     9841 ttttgggaag cataccctca acagcgagtt taataactgc ttctggcctt ctatttaata
     9901 atgagataag tttttcttct tttaaatgtc caagatatcc tgtaatatgt ttgtaataaa
     9961 ttttatctgt taattttttt cctgttacct ttatcttttc agcatttatt actatcacaa
    10021 aatctcctgt gtcaacattc ggagtataaa ttggtttatg ttttcccatt aagattttag
    10081 ccaccttact tgcaagccgt ccaagaatct gtccatctgc gtcaacaatg taccattttc
    10141 tttttattct ttctttcttt ggaacctgtg tttttgtcat ttttcctcct gtgttgatat
    10201 aaaaaacagg taatcggttt tttaaattta attaatttta actgaaaaaa aaattttcgt
    10261 caataaaatt taagattgac agaaagtaga aaaaaatata aattaagtaa aaagattttt
    10321 gaggaaaaaa tatgatagaa attttaattt ttctagttat tttatttttt tctattattg
    10381 ttcatgaagt atcacatggg tatgctgcat taaaaaatgg agacccaaca gccaaatata
    10441 tgggacgact tactttaaat ccaattccac atatagaccc tgttggtaca cttcttcttc
    10501 ctttccttct tattgtttta cattttccta ttttaatagg agttgcaaaa cctgttccaa
    10561 taaatcctta ttactttaga aattatagaa caggtatgat aactgttgga atttccggac
    10621 cattaagcaa tattcttctt ggaattattt ttgcttttat ttataaactt tttccatcca
    10681 gtattgtaag tggatattta aaaatgggaa gttttatcaa ttttattctt gcttttttta
    10741 atttaatacc aattcctcca cttgatggtt caagagtagt tgctgtttta ctaccttaca
    10801 aattaaggtt tggatatgaa aaaattgaaa ggtatggatt atttttagtt ctacttcttg
    10861 caatgacagg gtttttgaac tggctttctc ctttaagtca atctctcaca aattttttca
    10921 ttaatcttta aaacaaaatt ttttttccgg tataatctat ttaaaaaagg agggtttatg
    10981 aaatttaata taattgttcg tttttttgca gagatgggat ggcaggcact tggtaaaatt
    11041 tcaaaccctc tgacaggtaa aacagaaaaa aatcttgaga ttgcaaaaca ggtaatagaa
    11101 ttgcttgaaa cattgaaaga aaaaacgaaa gggaacctaa cagaagaaga agataaattt
    11161 ttaaactctg caattgccga tttacagtta aattatgttg aggaagtttc taagggaaaa
    11221 aaggaaaatg aatcagaaaa taaaacttaa attaaaaaca ttacccgatt ctcctggtgt
    11281 ttatttaatg aaggataaag aaggtaatgt tatttatgtt ggcaaagcat cttctttatt
    11341 aaaaagagtt cgttcttatt tctctccttc agggaacaat acaaaaatcc ttgcacttgc
    11401 tgaaaaaata tatgattttg aagttattcc tgttatttca gaagcggaag gtcttattct
    11461 tgaaaatcaa cttataaaaa aatacaagcc aaaatataat acaaatttaa aggatgataa
    11521 aagttatcct ttacttaaga taacaaaaga agaatttcct tcagttcaga tagtaagaga
    11581 gaaaaaaaca gataaagcaa tttattttgg tccatttaca aataaaaaac ttttgaaaga
    11641 gacagtcaga tttttgagaa aattttatcc tgtcagaaac tgtaaaaaga atgtttctac
    11701 tggaaaagta aaattgtgca ttcagtatca tataggtaga tgttcaggtc catgtgagaa
    11761 caaaataaaa aaagaagaat atcaaaaact tgttgaagga ataattgctt tttttgaagg
    11821 taaatataaa gaatttgaaa aaaaacttaa gaaatggatg ctggaagaga ttaaaaagtt
    11881 gaattttgaa gaggcagata aaattaaaaa gaggttgttt ctattgaatg aaatggcaga
    11941 aaaatttcct gtgagagagg aagaggaatt atataggtac agtgaagaaa atattttatt
    12001 caatctttca aagattttga aattgagtaa aataccgaat ttaatagaag gttatgatat
    12061 ttcaaatatt tcaggagatt ttgctgttgg aagtaaggtt tcttttattg ctggtattcc
    12121 atataaagat ggttacagaa gatataaaat aaaaactgtt gaaggaattg atgattataa
    12181 aatgcttgaa gaagttttaa taagaagatt tgatactgaa gaagagaaaa aacagattcc
    12241 tgatttaatt cttgtagatg gaggaaaggg acagttggga gttgcagttt ctgtattaaa
    12301 aaggtataat ttaaaaattc ctgttatttc acttgcgaaa agagaagaga aaatttttgt
    12361 tgagtggagt gaagagggta taattttacc tatttcttca cctgaattgc agttattaca
    12421 gagaatacga gatgaagccc atagatttgc tatttcttat cacagaaaga taaggagtaa
    12481 aaattttaaa ttttcttttc ttgatgaaat aaaaggaatt ggtgaaaaaa caaaaaaaag
    12541 aattattatg aattttcctg atgtaaatct tattgcaaat ttaaatgtag ttcaacttaa
    12601 acagatagga ataaatgaaa aaatagccga aaaaattatt gaaaaagcaa gggaggtgat
    12661 ttcaaatgaa aaatgagaaa atttttgaga aatttttaat ttttggcata ggaagtattt
    12721 attttattgg tgaaaatctg aaaagaatta tgaaggaaat tgaaaaggaa gggaatgaac
    12781 atgctgaatc aatagaaaag ataaagaagg aaatactgaa atttataaaa atgccaaaaa
    12841 atcttattgt agagttttta aaatcatgtg actttattac aaaagaagac cttgaaaaat
    12901 ttaaggaaga tataaaaaat ggttaaattt attgatgaag tacagatatt tgtgaaaagt
    12961 gggaaaggag gagatggttg tattgctttt agaagagaaa ggtgtattcc taagggaggt
    13021 ccatggggag gggatggtgg aaagggtgga gatgtaattt ttaaagcagt aaaaaataaa
    13081 aaaacactta ttgatttcca ttttcaaaaa catttttatg cagaaaacgg aagacctgga
    13141 agcagtaata ataagaaagg aaaagatgga gaggatttaa taattgaagt gccctgtgga
    13201 acaattgtaa aagaggttat aaatggagaa gaaactcttc tatcagacct tgttaacgaa
    13261 ggtgatactg ttattgttgc aaaaggtggt aaaggaggtc tcggtaatac tcattttaaa
    13321 tcgtcaacaa atcaagcacc aagaatagca acaaaaggag aacctggaga agagaaagta
    13381 ataaaacttg aattgaaact tattgcagat gttggaataa ttggttatcc aaatagtgga
    13441 aaaagtactc ttttatccaa aatttcaaaa gcaaaaccca aaatcgctga ttatcctttt
    13501 acaacccttg aaccaaatct tggacttgtt gatctcggag attttagaag ttttgttgta
    13561 gcggatatac ccggtttaat tgaaggtgct tcaaagggta aagggcttgg aattaaattt
    13621 ttaaaacata tagaaagaac aaaaattctt attcaccttt tagatttaag tgaaaatggt
    13681 ataataaata gataccagaa tataaggaaa gagttaaaaa attacagtga taaacttgtt
    13741 gaaaaaaaag aaattgttgt tggaaataaa atagaccttg aagagagtaa gaaaaatata
    13801 aaacttgcaa ccgattattt taaagaaata tattttattt ctgctttaaa aggagaagga
    13861 ataaaagaat tgcttgaaag aatatggaag gaattgaaaa atgattaaat tgaaggaaag
    13921 aatagaagat attaaaaagt actgggaaga aataaaggaa aaatttaaaa ttaaagagga
    13981 cttaaaaaca tttgaattac ttgaaaaaga aatgcatgaa aagggatttc ttgatgataa
    14041 aaacaaaata gaattatgga cttctttaaa agaaaaaaaa gaattagttg atgaaattga
    14101 gaaagacatt aaagaaagtg aaacattgtt tgaacttata gaggaaagta ccgatgagga
    14161 acttgaaaga gagggtgaag aaattttgaa aagattagaa gaaaatataa aaaaaattaa
    14221 atttgaatat atttggaagg aagaagatgt aaataatgca ataatcacat tgcattcagg
    14281 gacaggtggt acagaagcgt gtgactgggt aagtatgtta tggagaatgt acagtaagtg
    14341 ggcgtcaaaa aaaggatatg atgtaaaagt tattgatttt ttacctggtg aagaagcagg
    14401 attgaaaaga ataacagcaa taatttctgg aaaaaatgct tatggatact taaaaggaga
    14461 aacaggtgtt caccgtcttg ttagaatatc accctttgat gccaataaaa gaagacatac
    14521 aagttttgca ggagtaagtg ttattcctga ggttaaagaa gatataaaaa ttgaaataaa
    14581 ggatgaggat atagagattg aaacattcag ggctggtggt cacggaggac agaatgtaaa
    14641 taaagtggaa acagcagtaa gaataaaaca taaaccaaca ggaattgttg ttcagtgtca
    14701 gaatgaaaga agtcaattta aaaatagaga gattgcaatg aaaatattga aaagtagatt
    14761 gtatcaatta tatcttgaag agaaaaggaa aagtcaggaa gaaaaaagaa gtcaggagaa
    14821 agaaattaca tggggaagtc agataaggtc ctatattttc tgtccttata cacttgttaa
    14881 agaccacagg actgaagttg aaacatcaaa tattgaagga gttatggacg gcgatattga
    14941 tgaattttta gaggcagaga tagaatggct ggcaaaaaga taaaagttct tataagtgca
    15001 ggaccaacaa gagaatatat agaccctgtc agatttataa gtaatccttc cacaggaaaa
    15061 atgggttatt taattgctga atatgctaaa aaaaagggat ttgatgttat tcttgtgagc
    15121 ggtccaactt atttaaaacc acctgaaggt gtaaaattaa ttaaagttga aacagcagaa
    15181 gaaatgcaaa aagaaatttt aaaaaatttt ccaaaagttg atttactaat aatggcggct
    15241 gctgttagtg attggcgtcc ttttagaaaa ttcaaaaata aacttaaaag aaaaaatcaa
    15301 tggacactta aacttattcc aaatcctgat attcttaaaa gtgttgcaaa aataaaaaaa
    15361 gaaagtcaga aggttgttgg atttgcactt gaaacagaag atattataaa aaatgcagag
    15421 aaaaaattaa aagaaaagaa attagacctt attataggaa atacacctga tttttttgga
    15481 aaagggaaaa aatcagatgt atggtttata ttcaaagatg gtaaagtcat aaattttaaa
    15541 ataaaaaaag aacaactccc acctaaaata tttgaagttt ttttctaatt tttacttttg
    15601 ataaattttt gatataatca gatgaaaaaa ggagtgaaaa tgaatagaaa agttaaaatt
    15661 ggaattatag gagttggaca gattggaaaa agtcatataa aaaaatatga ggtcattcct
    15721 gatgcggaaa ttgtggcagt ttgtgatgtt aatgaggaag aagcaaaaag ggtctgtgaa
    15781 actcataata tcccgaaata ttatactgat tttagagaac ttttgaaaag ggaagatatt
    15841 gaagcagttg atgtgtgtct tcataataat ctacacatgc ttgttactgt tgaagcattg
    15901 aattcaggaa aacatgtata ctgtgaaaaa ccaatggctg gttcttatgc agatgcttta
    15961 aaaatgtatg aaacttccaa aaaaacaaaa aaatatttaa gcattcaact taatacttta
    16021 ttttcaaaag aaacaaaagc agcaaaaatt ttaattgaaa aaggatatct tggaaaaata
    16081 tactacggaa gaagtactgg ctacagacga cttggaagac cttttgttga tggatatggt
    16141 tcaaagtttt ttgttcaaaa agaagttgct gctggtggtg ccttatatga catgggagtt
    16201 tatcatattg caaatttact ttatcttatg ggaaatcctg aagttttaag aataagtgga
    16261 aaaacttatc aggaagttga aattaatgaa aaaagaaaag aaataagtaa ttgcaatgta
    16321 gaagaacttg gtataggatt tgttaaattt aagaataata tagtgatgga aatagtggaa
    16381 gcgtgggcaa tacatcttga cccttttgaa agttcataca tagttggaaa taaaggaggg
    16441 attcgtttaa atccattgcg atactttaca aatatagaag gagatatgcc aatggatgcc
    16501 acatttaatc ttgatatact ggattacaga tggcacaatg tttatgaaaa tggagatgtt
    16561 tatgacagtc cacaacatca ctggattgct gttcttcagg gcagggttga attattacca
    16621 acagcggaaa ttgctttaaa tacaatgttg atttcagaag gaatttattt atcagatgtt
    16681 cttggaagag aagtaacagc agaagaagta aaacagaatt caaaatcaac tgctttaaaa
    16741 atttaaaaaa aggaggaaaa gatgaaagta aaaatagcgg ttcagttgtt ttctgtgaga
    16801 aatgattgtg caaaggattt ttatgggact ttaaaagaag tggcaaaaat tggatatgaa
    16861 ggggttgaat ttgctggata ttatgaaaaa agtgcagaag aaataaaaaa gatgcttgat
    16921 gattttaatt taaaagttgc tggaactcat ataaggatag atactttact tggagatgaa
    16981 cttaaaaaaa caattgaatt taataggatt ataggaaata aatatctgat tgttccaatt
    17041 ttacctgaag aaatgagaaa ttcaaaagaa aaatggttag aggcagcaaa actattcaat
    17101 aaaatttcag atgaagtaag gaaagaaggg atgtatgttg gatatcacaa tcatgcagtt
    17161 gaatttcaga aaattgatgg tgaatatccc tgggatatat tttttaaaaa tacagtgcct
    17221 gatgttgtaa tg
//
LOCUS       DAOSAT010000029        17197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2295_length_17197_cov_5.700443, whole genome shotgun sequence.
ACCESSION   DAOSAT010000029 DAOSAT010000000
VERSION     DAOSAT010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17197
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2295_length_17197_cov_5.700443"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLF90_03780"
     CDS             <1..918
                     /locus_tag="PLF90_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56437.1"
                     /translation="LIKYEGKKKFTVINPYEDINWSTIKRYKANLHTHTTESDGKEKP
                     EKVISLYKMKNYSILSITDHNKFTSLKTDELFLIPGVEFGKNQQHHIVSLFSFKIPEK
                     IEALNEEEILKYVDEKEGICFFAHPGRYTKKIDWYVNLFEKYQSLSGIEVLNTGVQSK
                     GKIYGDTDIWDEILKKTMPERPVWGFGNDDFHTLSQLAINWNILLLEKIDEKEIKDAI
                     KKGKFYICSAVLGTDMPYFNRIIIDKKTGKIKIYCKNFKEIKWISDGKIVGYGSEINY
                     KKNTGIKKYLRVEIFGENGYIYLNPFGIK"
     gene            915..1988
                     /locus_tag="PLF90_03785"
     CDS             915..1988
                     /locus_tag="PLF90_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK56438.1"
                     /translation="MSKKIAIDAINLKMEDRVAHTEYSMEYHKDYISKITGMSEEYPE
                     RIKKFYEIWSYDFLWVTDDGLYGNWFDRGRATDMGHAIYAIDGSDRKEKKECPFKDVF
                     EVWEFDPEKEYSFPDFNEQVKTYQEKYKKLCEYFPDQLITGGYYKTIISGLIQTFGWE
                     MLLLSASDKNKFEKLIDRFFNYTLFYMKAWAYTDVDVIIQHDDFVWYSGPFLSPDFYR
                     KAIIPRYGELWKDLKKKGKKILFCSDGNFSMFAKDIIEVGADGLIFEPVNDFGFMAEN
                     FGDSICLVGSYVDCRDMTLGKWEKVKNDIERTLSYVKNGKCKGLIFAVGNHIPANVPD
                     WICENYINHLKKNWYLNYPTSHL"
     gene            complement(2040..2663)
                     /locus_tag="PLF90_03790"
     CDS             complement(2040..2663)
                     /locus_tag="PLF90_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOK56439.1"
                     /translation="MEKEYLLPTKICDVENPDIKMKSYFLTKKSFSEKQKAIDIFNFV
                     RDEIKYRFDYSYTKASETLKKGYGNCFNKSNLQIALLRSCGIYAGYRVYLIKKEVFLP
                     IVPKDIYEKISQPTVHVSCAVYIEKKWVESDSTIDFELYNHIYRNMENWKYVEWDGEN
                     DISIPAEYIVENQGIYSNIDLYLLNPPKFWNDELLEKANRFIDELLK"
     gene            2701..3039
                     /locus_tag="PLF90_03795"
     CDS             2701..3039
                     /locus_tag="PLF90_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56440.1"
                     /translation="MRKTIFYISLIFLTGCVFQKKEKTFLDAVYREEYIKNNPDLKEE
                     WKQSIIKGEIPPGMEKGEVEKILGKNHQTFISKTNLMEVWIYDKYYVGFDRNGKVIKF
                     GIFEKEEGEK"
     gene            3036..4016
                     /locus_tag="PLF90_03800"
     CDS             3036..4016
                     /locus_tag="PLF90_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK56441.1"
                     /translation="MKIIQIGVGGFGASWLDIVMSSKEWEVVAIVDINEEILKKSVEK
                     YPSLEGKTYTSLKDCLKKVKADALLNVTPPAFHKETSIMAMKEGLHVLVEKPLSDTMK
                     NAEKIVEICEKYGRKFMVSQNYRYNRIPRTIRKMVDNGEIGKISYCILNFQKGPRFGG
                     FRAEMPFPLLIDMSIHHFDLSRYLLNKDPVSVYAESWKPHWSWLKGDPCVYLIFDFED
                     DVHFNYAGSWVSVGKDTQWPGIWEIYGDRGTIIWDNSGIKKVVEGKEEIIEPVKLERE
                     DRFLSLYEFYRSIVEDREPETSGKDNLKSLGMVFKSLESIKKGKKLKVNL"
     gene            complement(4006..5286)
                     /locus_tag="PLF90_03805"
     CDS             complement(4006..5286)
                     /locus_tag="PLF90_03805"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOK56442.1"
                     /translation="MGILIKNGYLIEPKSGKEGKFDVLLEGGVVKEIKKNIPSRNHKV
                     IDCKNKIISPGFVDLHCHLREPGREDEETIYTGSLSAVSGGFTTICCMPNTEPPLDNK
                     VSVRFVYEKGEKSLCEVLPIGTITKGREGKEIAPYFEMIQEKAVAFSDDGDCVMDSNV
                     MRRALEYTLLHRKPVISHSEDKNLSKNGVMNEGFISTKMGLPGIPKEAEEIMVERDLK
                     LAKLTGGILHIAHLSCKESVESIKIAKKFLKNVSCEVTSHHLTLTEEVVIGYNPNAKV
                     SPPLRTEEDRIALIEGLKNGIIDCIVTDHAPHSFEEKESGFENAPFGIIGFETFLPLC
                     LKLIDYGMKLKDIIYKITYAPSKILNIERGIIEEGKRADIVIFDPDIEWVYTEDKVKS
                     KSKNSPFLNWKLKGKVLLTISRGKIVYREPEFTD"
     gene            complement(5321..6154)
                     /locus_tag="PLF90_03810"
     CDS             complement(5321..6154)
                     /locus_tag="PLF90_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK56443.1"
                     /translation="MEILFLGTAAAEGWPGIFCECEYCEKARELKSKNIRTRSSIIID
                     RKYKVDLPPDTYWHTVKYGLNLSKIEHLFITHSHADHFDFSELYFYFEPFAHFKNKER
                     YLNIYGNKNVFEIISNLIKEKKNGQFIIPHLLEVFKKYEAGDMEFYPVLADHEPKETS
                     LNFIFKYKNRTILHGYDTGWYPDITWEFIKDFKFDIVIMDCTNGILDFEKYHLGIKGV
                     IKVKEKLLKYGCLKEKFRFVATHFSHNGKLLYSELEKIFKKEKIEVAYDGKKIKLRDL
                     I"
     gene            6272..7384
                     /locus_tag="PLF90_03815"
     CDS             6272..7384
                     /locus_tag="PLF90_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK56444.1"
                     /translation="MTHREHFCKIMKGDVSNWIPNYEYGLWGQTFERWIKEGMPEDKI
                     YNNWFEGEPFFKIDRRGFAHINTGPVPAYQTEVIEETDRYIIQRNPWGYITKSLKEGT
                     VRGTRPSMDQYISFPVTDRESFNQMKEKYNPFSSVRYSLWWDEWVKIWKKRDYPLCLL
                     GNASWGIYSRLRIWVGTENLSYMFYDNPALVEEMIEFNVEFLLSLVEKALNEVEFDYF
                     NFFEDCAGKGGPLFGPEIFKKFFLKPYKRVIERLKKAGIEIITLDTDGNFEVLIPLWM
                     DAGINFFWPLEQASGMDPLKLRKKFGKNIILMGGIDKREIAKGKNEIEKELYKKIPPL
                     LEQGGYIPTLDHAISPDISYENFMYYIELKLKLIGR"
     gene            7386..8060
                     /locus_tag="PLF90_03820"
     CDS             7386..8060
                     /locus_tag="PLF90_03820"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOK56445.1"
                     /translation="MKNDFEIIAKYTDKPLIIDGKLQEDVWKKAPIYELELPGNEKKQ
                     PVEKGEVQLAWDEEYLYVGIKFYDSDIVQESNKNQTHHYLTGDLVEIFLKPEKNTWYW
                     EIYGTPNCKKTVFFFPGRGRAGLQGCFKPNIDLDRILIGTQIKGTLNNWHDIDEYWTL
                     EMAIPSEELKSFGDNFGYGSEWRILIARYNYSRYLHWKELSSFPKLSVPNFHLIEEYG
                     KLVFEK"
     gene            8072..9691
                     /locus_tag="PLF90_03825"
     CDS             8072..9691
                     /locus_tag="PLF90_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HOK56446.1"
                     /translation="MLYTEVNVPVEFSLRSYKRYKDIFNEVEVDVIFTSSKGEKFKVP
                     AFWKGENEFGVRVSFPEDGIYEWESISNNEKERGLHGQKGKIKVEKYKGKNPLFVHGR
                     LKISENKRYLCHKDGKPFFWLGDTWWMGLCKRLKWPEEFKELTDDRIKKGFTLIQIVA
                     GLYPDMSYPDRRGENEAGYPWDKNFKRINPYYFDMADLRLFHLINSGLISCIVGCWGY
                     YLKWMGIEKMKKHWRYLIARYSAYPVIWCASGEYDMPFYLSETSEEDKNLQRNGWKEV
                     IKYIKKIDPYKNPVTVHPGSFTREVEGYSEILDFDVLQTGHSDDSIKNTVYRVKQSYE
                     AEPVMPVVIGEVAYEGIGGQCREQIQRLSFWASFLNGACGFTYGANGIWQVNRKEEIY
                     GPSPHGMNWGNTPWDEAMSLPGSKQLGLSKKLLEKYRWWEFQPRPDWIEVDVSSENRE
                     KNYYPYIAGIPEEVRIVYIPFFYNNFKIKKIEKNVKYKTFLFNPVNGEKIDSGVVNPD
                     REGNWELPSLVKNSWAKLPIYQDWILVLERI"
     gene            9780..10841
                     /locus_tag="PLF90_03830"
     CDS             9780..10841
                     /locus_tag="PLF90_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOK56447.1"
                     /translation="MKKILSVIFLLFILGGCTPVLKQPELPKEEIETEREKQREIALN
                     TLIERQIRLYKTGFPLLKGAVNFYTKKPILSLGILIHSSKNYKKEDLPIISKKYRIED
                     SPTILYVHPHFGAYNAGLKINDRIIEINKKKVESIENISKIMEGIDLSKEKVEVIVDR
                     NGEILTFNVPTTKICPFSFVLYTDPQVGDSINASTDGQTIYATPGLLRFIQSDTELAF
                     VLSHEIAHAVLDHSVQKTGNRILGTIVDIAITVATGVNTQGVFGNIAGLVYSKEFEKE
                     ADYLGTYIAAISGYDVSDAPNFWRKMAAEYPGSTKDVFLTTHPSSPERYILIEKTVEE
                     IKEKQKKGIPLFPDYKKTK"
     gene            10845..11612
                     /locus_tag="PLF90_03835"
     CDS             10845..11612
                     /locus_tag="PLF90_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOK56448.1"
                     /translation="MSLILIGLITGFFAGFICGLFGMGGGSLMVPAIYHFFGFTFPTE
                     SRMKISIGTSLLVIIFSSISAIITYFKNRKIKTNLIILIVPSGILGSQFGAFLTSRLD
                     DETVKYIFIFLITFLGLRMFFAPENKENEISDNNSYNKLSGILIGFFSGLISGLCGVG
                     GAVLIIPLLYLFLKIPIHYAIGTALIAILFNAISGSIAYMVRNLVNIKIGLLLALTSI
                     IGAPLGAKIGINLPKKTMRKIFSIILILSGISVLFRK"
     gene            complement(11614..12711)
                     /locus_tag="PLF90_03840"
     CDS             complement(11614..12711)
                     /locus_tag="PLF90_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOK56449.1"
                     /translation="MEKDFDVIVIGAGVVGLSISYKLSERYSVGLIEKNKKYGLETSS
                     RNSEVIHSGIHYPENSLKAKLCVEGNNLLYEFLRENNILHKKTGKLTLAIEKDEIEEI
                     ERLYSQGIKNGVPELKIIEKNEIEKFCPEIEGICALYTPTTGILNVHYLMDCLYEKFI
                     NNGGIAGFDERVINIEKKNSLYLVETEKGRYYSEIVINCAGLYAENISRMLGFNYDVY
                     WAKGDYFTITRKFNIPLLVYPVPGKEGLGIHLTPRFDGILRAGPDVEYVEKIYPPYPD
                     EKVNSVYKIDDTKKEIFFEQLKKYLPSIKIDDLIPESYGIRPKLQKKGDEFKDFVIKE
                     EIPGFINLIGIDSPGLTCCISIAQYVKNIIL"
     gene            complement(12717..15098)
                     /gene="galB"
                     /locus_tag="PLF90_03845"
     CDS             complement(12717..15098)
                     /gene="galB"
                     /locus_tag="PLF90_03845"
                     /inference="COORDINATES: protein motif:HMM:NF041463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalB"
                     /protein_id="HOK56450.1"
                     /translation="MRKIEVLKYGWKFKKGDFQDAINFDFDDSNWEEVQVPHDWAIKG
                     PFSPENDKRVYVREENGRKKEIVLTGVTGGLPHAGKGWYRKKIELKNIKDKRIRIEFD
                     GVMSNSKVYCNGIYVGQWPYGYASFAFDITDFIKEGENLIAVSVDNKPNASRWYPGAG
                     IYRNVRLVIMNNVHIPLWGTYITTPYIDEKIAKVHIKTEVENHTGKSKYVELETKILS
                     PEGKEIITESTIKEISDKGIFEQEIFVENPLLWSVENPNLYTACLSVKIDGKVVDYYQ
                     TRFGIRTITFDSEKGFFLNGKDIKFKGVCMHHDLGPLGAAVNKIALKRQLTILKEMGC
                     NAIRTSHNPPCPELLDLADEMGFLVIDEAFDEWKIPKCENGYNKLFDEWAEKDLRAMI
                     RRDRNHPSVIMWSIGNEIPEQTDPVNGPKLAKFLHNICKEEDPTRPTTTAMNWGEEAI
                     KNGFAQVVDVCGWNYLPHLYGKFHQLLPGKPMYASETASCISTRGEYYFPVEEERDVK
                     RETLQVNSFDLSHPSWANIPDVEFKAQDEHPFIMGEFVWTGFDYLGEPTPYNEEWPSR
                     SSYFGIVDLCGIPKDRFYLYQSIWADKETLHIVPHWTLFGFEGKAITVQVYSKWNTVE
                     LFVNGISYGKKTKHSRGLVNRYRLVWNGVIYQPGEVKAVVYDEKGNVVKETVVKTAGK
                     PSKIKLIPDREIIKGDGEDMVFVYVEVVGEKGVLCPFADNLIHFDIKGPAEIVAVDNG
                     NPISTEPFCANYRKVFHGKCVVYIRSIFKKKGKIKITAKSEGLKGAQIIIKAE"
     gene            complement(15438..15521)
                     /locus_tag="PLF90_03850"
     tRNA            complement(15438..15521)
                     /locus_tag="PLF90_03850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15486..15488),aa:Leu,seq:tag)
     gene            complement(15834..17186)
                     /gene="radA"
                     /locus_tag="PLF90_03855"
     CDS             complement(15834..17186)
                     /gene="radA"
                     /locus_tag="PLF90_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOK56451.1"
                     /translation="MKKEKSIFVCSECGYQTVKLLGRCPNCGKWNTFIEEFYTSLEKF
                     EEKEIVHPKKLSEIEFEETPRIKTGIEEFDRVLGGGIVKKSYILVAGEPGVGKSTLLL
                     TVCGNLAKNGYKVLYISGEESENQIKMRSKRLGVESDEIYLLTTSEINTIKNGLEKIK
                     PDFIIIDSIQTIYNPEIPTIPGSVTQVRENANFFMKYTKANNCSTFLVGHITKEGIVA
                     GPKILEHIVDAVIYFEGDIRTNLRILRSIKNRFGSTNEIGVFSMEENGLKEIPDASSI
                     FIQDIEKNTPGRTIFPTIEGTRTILVEIEGLVTPTYYGIPKRTVSGLDYNRVSLIIAV
                     LEKKLKFNFNTYDVYVNVGGGIKINEVGSDLGVAVSCVSSLKDIAPLKKSVVIGEIGL
                     TGEIRGVENINLRLKECARLGIEKAIIPEKNKNDVFVKNIEIYPVRFLYEAIQISLNL
                     "
BASE COUNT         5929 a         2707 c         2591 g         5970 t
ORIGIN      
        1 ctgattaaat atgagggaaa gaaaaagttc actgttataa atccttatga agatataaac
       61 tggagtacaa ttaaaagata caaagcaaac ttacatactc atacaacaga aagtgatgga
      121 aaagaaaaac ctgaaaaagt tatttctctt tacaaaatga aaaactattc aattttatca
      181 atcacagacc acaataaatt tacatcttta aaaacagatg aactattttt aatacctgga
      241 gttgagtttg gtaaaaatca acagcatcat atagtttccc tcttttcatt caaaataccc
      301 gaaaaaatag aagcattaaa tgaagaggag attttaaaat atgttgatga aaaagaagga
      361 atttgttttt ttgcgcatcc aggaagatac acaaaaaaaa ttgactggta tgttaattta
      421 tttgaaaagt atcagagttt atcgggaatt gaagttctaa atacaggtgt tcagagtaaa
      481 ggtaaaattt atggagatac agatatatgg gatgaaattt tgaaaaaaac aatgcctgaa
      541 agacctgttt ggggatttgg aaatgacgat tttcacactt tatctcaact tgcaataaac
      601 tggaatattc ttttacttga aaaaattgat gaaaaagaaa taaaagatgc aataaaaaag
      661 gggaaatttt atatatgctc tgctgtgtta ggaactgata tgccttactt taatagaata
      721 attattgata aaaaaacagg aaaaattaaa atatattgta aaaattttaa agaaataaaa
      781 tggatttcag acggaaaaat tgttggttat ggaagtgaaa taaattacaa aaaaaatact
      841 ggaataaaaa aatatttaag agttgaaatt tttggagaaa atggatatat atacttaaac
      901 ccatttggta taaaatgagc aaaaaaatag caattgatgc gataaatctt aaaatggaag
      961 atagagttgc acatacagaa tacagtatgg aatatcataa agattacatt tcaaaaatta
     1021 caggtatgtc agaagaatat cctgaaagaa ttaaaaaatt ttatgaaata tggagttatg
     1081 actttttatg ggtaactgat gatggacttt acggaaactg gtttgataga ggaagagcaa
     1141 ctgatatggg acatgcgata tatgcaatag atggttctga tagaaaagaa aaaaaagaat
     1201 gcccttttaa agatgttttt gaggtatggg aatttgaccc tgagaaagaa tatagttttc
     1261 ctgattttaa tgaacaggtt aaaacatatc aagaaaaata taaaaaactc tgtgagtatt
     1321 ttccagacca acttataaca ggaggatatt acaaaacaat tatttcagga ctgatacaga
     1381 cattcggatg ggaaatgctt ttactctctg caagtgacaa aaataaattt gaaaaactta
     1441 tagatagatt ttttaactat actctttttt atatgaaagc atgggcttat acagatgtag
     1501 atgtaataat ccagcatgat gattttgtat ggtattcagg tccttttcta tctcctgatt
     1561 tttacagaaa agcaatcata ccgagatatg gagaactctg gaaggattta aagaaaaaag
     1621 ggaaaaaaat actcttttgt tcagacggaa atttcagtat gtttgcaaaa gatataattg
     1681 aagtaggtgc agatggactt atttttgaac cggtaaatga ttttggtttt atggctgaaa
     1741 attttggtga tagtatttgt cttgttggaa gttatgttga ttgcagagat atgacacttg
     1801 gaaaatggga aaaagtaaaa aatgatattg aaagaacttt aagttatgta aaaaatggaa
     1861 aatgtaaggg tttaatcttt gctgttggaa atcacatacc ggcaaatgta cctgactgga
     1921 tatgtgaaaa ttacataaat catcttaaaa aaaactggta tctaaattac cctacctccc
     1981 acctttaagg tggacttggg aaattgttga cagacaaagg tttcaaaagg gacttttttt
     2041 tattttaaaa gttcatctat aaatctattt gctttttcaa gtaattcatc attccagaat
     2101 ttaggtggat tcaaaagata aaggtcaatg ttggaataaa taccctgatt ttcaactata
     2161 tactccgctg gtattgatat atcattttct ccatcccatt caacatattt ccagttttcc
     2221 atatttctgt aaatatggtt atataactca aaatctattg ttgaatcact ttccacccat
     2281 tttttttcta tataaacagc acaacttaca tgaacagttg gctgacttat tttttcgtaa
     2341 atatccttag gaacaattgg taaaaagacc tcctttttta tcagatacac tctatatcca
     2401 gcatatatac cacaacttct taaaagcgct atttggagat ttgatttatt aaaacaattt
     2461 ccatatcctt ttttcaatgt ttccgacgcc tttgtatagg aataatcaaa cctgtattta
     2521 atttcatctc tcacaaaatt aaaaatatca attgctttct gcttttcaga aaaactcttt
     2581 tttgtgagaa aataactttt catctttata tcgggatttt caacatcaca gatttttgta
     2641 ggtaatagat attctttttc cataatatat atgataaaat aaaaaacaaa aggagggaag
     2701 atgcgaaaaa caatttttta tatttcttta atttttctta ctggatgtgt atttcaaaaa
     2761 aaagaaaaaa catttcttga tgctgtttat agagaagaat atataaaaaa taaccctgat
     2821 ttaaaagaag agtggaaaca gtcaattatt aaaggagaaa taccacctgg gatggaaaaa
     2881 ggtgaagttg aaaaaatact cggtaaaaat catcaaactt ttatttcaaa aactaacttg
     2941 atggaggtat ggatttatga taaatattat gtaggttttg acagaaacgg gaaagtaata
     3001 aaattcggaa tttttgaaaa agaggaggga gaaaaatgaa gattatacag attggagttg
     3061 gtggatttgg agcaagttgg cttgatatag ttatgagttc aaaggaatgg gaagttgttg
     3121 ccatcgttga tataaatgaa gaaattttaa aaaagagtgt tgaaaaatat ccatctcttg
     3181 aaggaaaaac atacacttct ttaaaagatt gtttgaaaaa agttaaagcg gatgcacttt
     3241 taaatgttac tccacctgct tttcacaaag aaacttcaat tatggctatg aaagaaggac
     3301 tccatgttct tgttgaaaaa ccattatctg acacaatgaa aaatgccgaa aaaattgttg
     3361 agatatgtga aaaatatgga aggaaattta tggtaagtca gaactacaga tacaatagaa
     3421 ttccaagaac tataagaaag atggttgata atggtgaaat aggaaaaata agttactgta
     3481 ttttaaattt tcagaaagga ccaagatttg gtggttttag agcagaaatg ccatttccac
     3541 ttttaattga tatgtcaata catcattttg atttatcaag atatctactt aataaggacc
     3601 ctgtgagtgt ttatgcagag agctggaaac ctcactggag ttggttaaaa ggagacccat
     3661 gtgtttattt aatttttgat tttgaagatg atgtacattt caattatgct ggaagttggg
     3721 taagtgttgg aaaggatact caatggcctg ggatatggga aatttatgga gacagaggaa
     3781 caataatatg ggataactca ggaataaaaa aagttgttga aggaaaagaa gaaattattg
     3841 aaccagttaa acttgaaaga gaggacagat ttctttctct ttacgaattt tacagaagta
     3901 ttgtagaaga tagagaacct gaaacaagtg gaaaggataa tcttaaaagt ttgggtatgg
     3961 tgtttaaatc acttgaatca ataaaaaagg ggaaaaaatt aaaagttaat ctgtaaattc
     4021 aggttctcta taaacaattt tccctctact gatagtcaat aaaactttgc cttttagttt
     4081 ccagtttaaa aatggagaat ttttactttt actttttact ttatcttctg tataaaccca
     4141 ttcaatatca gggtcaaaaa ttacaatatc tgctctttta ccctcttcta ttattcccct
     4201 ctctatattt aaaatttttg atggagcata agttatttta tatattatat cctttaattt
     4261 cattccataa tcaataagtt tcaaacacaa aggtaaaaat gtctcaaaac caattatccc
     4321 aaaaggtgcg ttttcaaatc cagattcttt ttcctcaaaa ctgtgaggtg catggtctgt
     4381 tacaatacag tcaattattc cattcttcaa tccttcaatc aaagcaatcc tatcttcttc
     4441 tgttcttaaa ggtggagaaa cctttgcatt tggattatac ccaataacca cctcttccgt
     4501 taaagtcaga tgatgtgacg taacttcaca ggatacattt tttagaaatt ttttagcaat
     4561 ttttattgac tcaacgcttt ctttacagga taggtgtgca atatgtaaaa ttccgcctgt
     4621 taattttgca agttttaaat ctctttcaac cattatttct tctgcttctt ttgggattcc
     4681 tggaagtccc atttttgtag aaataaatcc ttcattcata accccatttt ttgacagatt
     4741 tttatcctct gaatgtgaaa taacaggttt tctgtgtaaa agtgtatact caagagccct
     4801 tctcataaca ttactgtcca taacacaatc accatcatca gaaaatgcta ctgctttttc
     4861 ctgtatcatc tcaaaatatg gcgctatttc tttaccttct cttccctttg taatcgttcc
     4921 aattggtaaa acttcgcata aactcttttc tcccttttca taaacaaatc ttacactgac
     4981 cttattatca agaggaggtt cagtatttgg catacaacaa attgtcgtaa atccgccact
     5041 tactgctgat aaactccctg tgtaaatcgt ttcttcatct tctcttcccg gttctcttaa
     5101 atgacagtgt aaatcaacaa atccaggtga aataatttta tttttacaat caattacttt
     5161 atgatttctg gaaggaatat tttttttaat ttctttaacc actccacctt caagtagtac
     5221 atcaaattta ccttccttac ccgacttagg ttctataaga tatccatttt ttatcaatat
     5281 acccatattt ttcaaaatta tattttattt caaatttttg tcaaattaaa tctcgtaatt
     5341 ttattttctt accatcataa gcgacttcaa ttttttcttt tttaaatatt ttctcaagtt
     5401 ctgagtaaag taatttcccg ttatgagaaa aatgtgttgc tacaaatcta aatttttctt
     5461 ttaaacatcc atacttcaaa agtttctctt taacttttat aactcccttt attccgagat
     5521 gatatttttc aaaatcaaga ataccatttg tacaatccat aataactata tcaaatttaa
     5581 aatcttttat aaattcccat gtaatatcag gataccatcc agtatcatat ccgtgaagaa
     5641 tagttctgtt tttatattta aatataaaat taagggatgt ttcttttggc tcatggtcag
     5701 caagtactgg atagaattcc atatctcctg cttcatattt tttaaaaact tccaacaaat
     5761 gaggaattat aaattgccca tttttcttct cttttatcaa atttgatata atttcaaaaa
     5821 catttttatt tccatatata ttcagatacc tttctttatt tttaaaatga gcaaagggtt
     5881 caaaataaaa ataaagttct gaaaaatcaa aatggtctgc atgtgaatga gttataaaaa
     5941 gatgttctat ttttgataaa ttcaatccat attttacagt gtgccagtaa gtatcaggtg
     6001 gtaaatctac tttatatttt ctatcaatta taatagaaga ccttgtacga atatttttac
     6061 tctttaattc ccttgctttt tcacagtact cacattcaca gaaaatgcca ggccaacctt
     6121 cggctgccgc agttccaaga aaaagtattt ccatatttat tttccttttt tctggtaaat
     6181 ttaaatcaat ttcagcactt ctgtcaaatt ttttaatttt taagtaaatc taaaaagttg
     6241 taaaatagaa aagattaaaa gggaggtttt tatgacacac agagaacatt tctgcaaaat
     6301 tatgaaagga gatgtaagta actggattcc aaattatgaa tatggattat ggggtcagac
     6361 atttgaaaga tggataaaag aaggaatgcc tgaagataaa atatacaata actggtttga
     6421 aggagaacca ttctttaaaa ttgatagaag aggatttgct catataaata caggacctgt
     6481 tccagcatac caaacagaag taattgaaga aacagataga tatatcatac agagaaatcc
     6541 atggggatat ataacaaaat cactaaaaga aggaacagta agaggaacaa gaccttcaat
     6601 ggaccagtat atatcatttc cagttacaga cagggaaagt tttaatcaga tgaaagaaaa
     6661 atacaatcct ttttcatctg taagatattc tttatggtgg gatgaatggg taaaaatatg
     6721 gaaaaaaagg gactatccat tatgtctttt aggaaatgca tcatggggaa tttactctcg
     6781 cttaagaatc tgggtaggaa ctgaaaatct gtcatatatg ttttatgata atcctgcact
     6841 tgtagaagaa atgatagaat ttaatgttga atttttactt tcacttgttg aaaaagcact
     6901 taatgaagtt gaatttgatt attttaattt ttttgaagat tgtgcaggta aaggaggtcc
     6961 attatttggt cctgaaatat ttaaaaaatt cttcttaaaa ccttataaac gagtgattga
     7021 aagattgaaa aaagcaggaa ttgaaataat tacacttgat acagatggaa attttgaagt
     7081 acttattcct ttatggatgg atgctggtat taacttcttc tggccacttg aacaggcaag
     7141 cggaatggac cctttaaaat tgagaaaaaa atttggtaaa aacattattc ttatgggtgg
     7201 aattgataaa agggaaattg caaaggggaa aaatgaaatt gaaaaagaat tgtataagaa
     7261 aatcccacct ttacttgaac agggtggata tataccaact ttagaccatg caataagtcc
     7321 tgatatatct tacgaaaatt ttatgtatta tattgaattg aaattgaaac ttatcgggag
     7381 gtaagatgaa aaatgatttt gaaattattg ctaaatatac agataaacct ttaattattg
     7441 atgggaaact acaagaagat gtatggaaaa aagcacctat ttatgaactt gaacttccgg
     7501 gtaatgaaaa aaaacaacca gtagaaaaag gagaagttca acttgcatgg gatgaagagt
     7561 atttatatgt aggaattaaa ttttatgatt cagatattgt tcaggaaagt aataaaaatc
     7621 agacacatca ttatcttaca ggagaccttg ttgaaatatt tttaaaacct gaaaagaata
     7681 catggtactg ggagatatat ggaactccaa attgtaaaaa aactgttttc ttttttcctg
     7741 gaagaggaag agcaggatta caggggtgct ttaaaccaaa tattgacctt gacagaattc
     7801 ttataggaac acagataaaa ggaacattaa ataactggca tgacatagat gagtactgga
     7861 cacttgaaat ggctatccct tctgaagaat tgaaatcttt tggtgataat tttggttatg
     7921 gttcagaatg gaggattttg atagcaagat ataattattc aagatatttg cactggaagg
     7981 aactatcttc ttttcctaaa ctttctgttc ctaactttca tcttattgaa gaatatggta
     8041 aacttgtatt tgaaaaataa aaggggataa aatgttatac acagaagtca atgttcctgt
     8101 tgagttttct ttaagaagtt ataaaagata taaggacatt tttaatgaag ttgaagttga
     8161 tgttattttt acctccagta aaggagaaaa atttaaagtt cctgctttct ggaaaggaga
     8221 aaatgaattt ggtgtaaggg tatcttttcc tgaagatgga atttatgaat gggaaagtat
     8281 atctaacaat gaaaaagaaa gaggattaca cggacagaaa ggaaaaataa aagttgaaaa
     8341 atataaagga aaaaatcctc tttttgttca tggaagatta aaaatttctg aaaataaaag
     8401 gtatttatgt cacaaagatg gcaaaccatt tttctggttg ggtgatacat ggtggatggg
     8461 tttatgtaaa aggttgaaat ggcctgaaga atttaaggaa cttacagatg atagaatcaa
     8521 aaagggtttt actttaattc aaattgttgc tggtttatat cctgatatga gttatcctga
     8581 tagaagagga gaaaatgaag caggatatcc atgggacaaa aatttcaaaa gaatcaatcc
     8641 ttattatttt gatatggcag atttaagatt atttcatcta attaattcag gacttatatc
     8701 gtgtattgtt ggatgctggg gatattattt aaaatggatg ggaattgaaa aaatgaaaaa
     8761 gcactggaga tatttaatag caagatattc tgcttatcct gttatatggt gtgcttctgg
     8821 tgaatatgat atgccatttt atttatcaga aacttctgag gaggacaaaa atttacaaag
     8881 gaatggttgg aaagaagtaa taaaatatat aaaaaagatt gacccataca aaaatccagt
     8941 aacagttcat ccaggcagtt ttacaagaga ggttgaagga tattcagaaa ttcttgattt
     9001 tgacgtgctt cagacaggtc attcagacga ttcaatcaaa aatacagttt acagagtgaa
     9061 gcaatcttat gaagcagagc ctgtaatgcc tgttgtgatt ggagaagttg cttatgaagg
     9121 aataggaggt cagtgcagag aacagataca gagattgagt ttctgggcat cttttttaaa
     9181 tggtgcttgt ggatttactt atggagcaaa tggaatatgg caggtaaata gaaaagaaga
     9241 aatttatggt ccatctccac atggtatgaa ctggggaaat actccatggg atgaggcaat
     9301 gagtttacct ggttcaaaac aacttggatt atcaaaaaaa ttacttgaaa aatatagatg
     9361 gtgggaattt caaccaagac ctgactggat tgaagttgat gtgagtagtg aaaacagaga
     9421 aaaaaattac tatccataca ttgctggaat tcccgaagaa gtccgtattg tttatattcc
     9481 ttttttttac aataacttta aaatcaagaa aattgagaaa aatgttaaat ataaaacatt
     9541 tctttttaat cctgtaaatg gtgaaaaaat agatagcgga gttgtaaatc ctgacagaga
     9601 aggaaactgg gaattgcctt ctcttgttaa aaactcatgg gcaaaactcc ctatctatca
     9661 ggactggatt ttagttcttg aaagaattta gtttttttat ttatatcatc ctttttttga
     9721 ctttaacata aattttaaat ataattttaa attagaagga caaaaatggg atgaaaaaaa
     9781 tgaaaaaaat tttaagtgtt atttttctat tatttatttt aggaggatgt actcctgttt
     9841 taaaacaacc agaactccca aaagaagaaa ttgaaacaga aagagaaaaa caaagggaaa
     9901 ttgctctcaa tacacttatt gagagacaga taagattata taaaactggt tttccattat
     9961 taaaaggtgc tgttaatttc tacacaaaaa aaccgatcct ttcacttggg attcttattc
    10021 atagcagtaa aaattataaa aaagaagatt tacctattat aagtaaaaaa taccggattg
    10081 aagattctcc aaccatactt tatgtacatc cacatttcgg ggcttataat gcaggattga
    10141 aaataaacga cagaataatt gagataaata aaaagaaagt agaaagcata gaaaatattt
    10201 caaaaattat ggagggaatt gatttaagta aagaaaaggt tgaagttatt gttgatagga
    10261 atggagaaat tttaactttt aatgtcccta caacaaaaat atgtccattt tcttttgttc
    10321 tttatacaga ccctcaggtt ggagatagca taaatgcttc tactgacgga cagaccatat
    10381 atgcaactcc gggattatta aggtttattc aatcagatac agaacttgct tttgttttat
    10441 cacatgaaat tgctcatgct gttttagacc attctgttca aaaaactggc aacagaattc
    10501 tcggaacaat tgttgacatt gcaataactg ttgcaacagg tgttaatact cagggtgttt
    10561 ttggaaatat tgcaggtctt gtttattcaa aagaatttga aaaagaagca gattatcttg
    10621 gaacctatat cgctgctata agtggatatg atgtttctga tgctcctaat ttctggagaa
    10681 agatggctgc tgaatatccc gggtcaacaa aagatgtttt tcttacaact catccatctt
    10741 ctcctgaaag gtatatttta attgaaaaaa cagttgaaga aattaaagaa aaacagaaaa
    10801 aagggattcc tcttttccct gactacaaaa aaaccaagta aaaaatgtct ttaattttaa
    10861 tcggacttat aacaggattt tttgcaggtt ttatatgtgg actttttggt atgggtggag
    10921 gttctttaat ggttcctgca atatatcatt tctttggttt tacttttcca actgaaagca
    10981 gaatgaaaat ttctatcgga acatctttac tcgtaattat tttttcctca atatctgcaa
    11041 tcataactta tttcaaaaat agaaaaatta aaacaaacct tattattttg attgtccctt
    11101 ctggaatttt aggttctcaa tttggtgcat ttttaacaag cagattagat gatgaaactg
    11161 taaaatatat ctttattttt cttatcacat ttttgggttt aagaatgttt tttgctcctg
    11221 aaaataagga aaacgagata tcagataata attcatataa caaactttct ggaattttaa
    11281 ttggtttttt ttctggttta atctcaggcc tgtgtggagt tggaggagca gttttaataa
    11341 tacctttact ttatttattc ttaaaaattc caattcatta tgcaatagga actgcactca
    11401 tcgcaatact ttttaatgca atttcaggaa gtatcgccta tatggtcaga aatcttgtaa
    11461 acattaaaat aggactattg cttgcattga cttctattat aggtgctcct cttggtgcaa
    11521 aaattggtat aaatcttccc aaaaaaacaa tgagaaagat tttctcaata attcttattt
    11581 tatcgggtat ttcagtcctt ttcagaaaat aactcacaaa ataatatttt ttacatattg
    11641 agcaattgaa atacagcagg tcaaacctgg tgaatcaata ccaattaaat ttataaatcc
    11701 aggtatttct tcttttataa caaaatcttt aaattcatct ccttttttct gcaattttgg
    11761 tcttataccg taactttcag gaattaaatc atcaattttt atagaaggta aatatttttt
    11821 caattgctca aaaaaaattt ctttttttgt atcgtcaatc ttatatactg aatttacttt
    11881 ttcatccgga tatggtggat aaattttttc aacatattca acatcaggac ctgctcttaa
    11941 aattccatca aatctcggag ttaaatgaat tcccagtcct tctttacctg gaacaggata
    12001 aacaagcaaa gggatattaa attttcttgt aattgtaaaa taatctcctt ttgcccagta
    12061 aacatcataa ttgaatccga gcattcttga gatattttca gcatatagcc ctgcacaatt
    12121 tataacaatt tcagaataat atctaccttt ttctgtttca acaagatata aagaattttt
    12181 cttttcaata ttgataaccc tttcatcaaa tccagcaatc ccgccattgt ttataaattt
    12241 ctcatataga cagtccataa gataatgaac atttaaaatt cctgttgtag gagtatataa
    12301 agcacatatt ccttctattt caggacaaaa tttttcaatt tcatttttct caattatttt
    12361 taattcagga actccatttt ttattccctg actatataat ctctcaatct cttctatctc
    12421 atccttttct attgctaatg taagttttcc cgtttttttg tgtaatatat tattttctct
    12481 taaaaactca taaagtaaat tattcccttc aacacacaat tttgctttta atgaattttc
    12541 tggataatga attcctgagt gtattacttc actatttctt gaacttgttt caagaccata
    12601 ttttttattt ttctcaatta aaccaactga atatctttca gaaagtttat aagaaattga
    12661 aagtccaaca actccagccc ctataacaat aacatcaaag tccttttcca tttaatttat
    12721 tctgctttta ttataatctg tgctccttta agtccttctg atttagcagt aatttttatc
    12781 tttcctttct ttttaaaaat tgaccttata taaacaacac attttccatg aaaaactttt
    12841 ctgtaattag cacagaatgg ttctgtagat attggatttc cattatcaac agcaactatt
    12901 tcagcaggac cttttatatc aaaatgaatt aaattatcag caaaaggaca taaaactcct
    12961 ttctcaccaa cgacttccac atatacaaaa accatatcct ctccatcccc tttaattatt
    13021 tctctatcag gaataagttt aatctttgat ggttttcctg ctgttttcac aactgtttcc
    13081 tttactacat ttcctttttc atcatataca actgctttta cctcacccgg ctgatatata
    13141 actccattcc ataccaatct atatctattc acaagtcctc ttgaatgttt agttttctta
    13201 ccataggaga taccatttac aaaaagttca acagtattcc atttactgta aacctgaaca
    13261 gtaattgctt taccttcaaa accaaaaagg gtccagtgag gaacaatatg taatgtttct
    13321 ttatctgccc atatactctg atataaataa aacctgtcct ttggtatacc gcataaatca
    13381 actattccaa aatatgaact cctgcttggc cattcttcat tatacggggt tggttctcca
    13441 agataatcaa aacctgtcca gacaaactca cccataataa aaggatgctc atcctgtgct
    13501 ttaaactcaa catccgggat atttgcccat gaaggatgtg ataaatcaaa actgttcacc
    13561 tgcaatgttt ctcttttaac atctctttcc tcttcaactg gaaaataata ttctcctctt
    13621 gtacttatac atgatgctgt ctcacttgca tacataggtt ttccaggaag aagttgatga
    13681 aatttcccat aaagatgtgg taaataattc catccacaga catcaacaac ctgtgcaaaa
    13741 ccatttttta ttgcttcttc tccccagttc attgcagttg ttgtaggtct tgtagggtct
    13801 tcttccttac atatattgtg taaaaattta gcaagtttag gtccgttaac agggtctgtc
    13861 tgttcaggaa tttcatttcc aatactccac ataataacgg atggatgatt tctatctctt
    13921 cttatcattg ctcttaagtc cttttctgcc cattcatcaa acaacttatt atatccattt
    13981 tcgcatttag gtattttcca ttcatcaaac gcctcatcta tcacaagaaa acccatttca
    14041 tctgcaaggt caagaagttc aggacatgga ggattatgac ttgttcttat cgcattacat
    14101 cccatttctt ttaaaattgt aagttgtctt tttaaagcaa ttttatttac tgctgctcca
    14161 agaggtccaa ggtcatgatg catacaaact cctttaaatt taatatcttt tccatttaaa
    14221 aagaaaccct tttcgctgtc aaaagtaatt gttcttatcc caaatcttgt ctggtaataa
    14281 tcaaccactt ttccatctat ttttacactc aaacatgcag tataaagatt gggattttca
    14341 actgaccaca ataatggatt ttcaacaaaa atttcctgtt caaatattcc cttatcagag
    14401 atttccttga ttgtactttc tgtaattatt tctttccctt caggtgataa aatttttgtt
    14461 tcaagttcaa catatttact ttttcctgta tgattttcaa cttctgtctt tatgtgaact
    14521 tttgcaattt tttcatcaat ataaggagtt gtaatatatg ttccccataa aggaatgtgc
    14581 acattattca ttataacaag acgaacattc ctgtaaatcc ctgctcccgg ataccacctt
    14641 gaagcattcg gtttattatc aactgaaact gcaataagat tttctccttc tttaataaaa
    14701 tctgtaatgt caaaagcaaa agaagcataa ccataaggcc attgtccaac ataaatccca
    14761 ttacaataaa ctttactatt actcataaca ccatcaaact caatccttat tcttttatct
    14821 ttaatgtttt taagttcaat tttttttctg taccaacctt taccagcatg aggaagacca
    14881 cctgtaacac cagtaagaac aatttctttt tttcttccat tttcttccct aacataaacc
    14941 cttttatcat tttccggact gaaaggacct tttattgccc agtcatgagg aacctgtact
    15001 tcttcccagt tagaatcatc aaaatcaaaa tttattgcat cctgaaaatc tcccttttta
    15061 aacttccatc catattttaa aacttctatt ttcctcattt tttccctcca tgtaaatact
    15121 ttattttaac caatttcccc ttaaagtcaa aaacaaactt cctcttaaag ttaaattttt
    15181 attttactac cagatttcca aatctcttac atatttatat aacccacctt taaggtggac
    15241 tgaacaattc gaataatcac tcagggtgat gcaaaatcaa agccagaaaa ttttgaccct
    15301 caaattttag agatatttaa gaaataccat aaggaattca aagaaatatt tgataaaaat
    15361 tcctgagttt actaagtccc ttcctcttaa ttatgtttcc cgacatctcc actgcccacc
    15421 ttaaaggtgg gagaaaatgc gaggggaggg atttgaaccc tcatccgcct aaggcggact
    15481 ggatcctaag tccagcgcgt ctgccattcc gccaccctcg cttttatatt tttcttctat
    15541 ttctactgcc gagtccagtt tttacttctt cttaagttgg ttacagttgt gctcctgatt
    15601 atatttctac tcctttcctc tatccttcac ttacttacgt ctctatatca tattacatcc
    15661 tacccgcacc aactcgcccc ttgctacacc caccttgcta atcagggctt actacagggg
    15721 ctcggttccg ccaccctcgc ttttatattt ttctttctga aaaattctat aattttattt
    15781 tactcctgtt tgcgtaagag tgtccaattt ttacaaggga tgcccctgtg aaattatagg
    15841 tttaaggaaa tctggattgc ttcatataaa aatctgacag gataaatttc tatattttta
    15901 acaaaaacat catttttatt tttttcagga ataatggctt tttcaattcc aagacgtgca
    15961 cattctttca atcttaaatt tatattctca actcctctta tttctcctgt aagccctatt
    16021 tctccaatta caacagattt ttttaaaggt gcaatatctt taagtgaaga aacacaggaa
    16081 acagcaactc caaggtcact acccacttca tttattttta ttccaccgcc aacattgaca
    16141 taaacatcat atgtattgaa attgaatttt aattttttct caagaactgc aattattaaa
    16201 gaaaccctgt tataatcaag ccctgaaaca gttctttttg gaattccata atatgtcgga
    16261 gttacaagtc cttcaatttc aacaagtatc gttcttgttc cttcaatcgt agggaatata
    16321 gtccttccag gtgtattttt ttcaatatcc tgaatgaaaa tacttgaagc atcaggtatt
    16381 tctttaagtc cattttcttc cattgaaaaa acgccaatct catttgttga gccaaatctg
    16441 ttttttatac tccttaaaat tcttaaattt gttcttatat ctccttcaaa ataaataact
    16501 gcatcaacaa tatgctcaag aattttaggt cccgcaacaa tcccttcttt tgttatatgt
    16561 cctacaagaa aagtggaaca attatttgct tttgtgtatt tcataaaaaa atttgcattt
    16621 tctcttacct gggtaactga acctggaatt gtcggtattt ctggattgta aattgtctgg
    16681 attgaatcaa ttataataaa atccggcttt attttttcaa gtccattttt tattgtattt
    16741 atttctgaag ttgtcaataa atatatttcg tcactctcaa ctccaagtcg tttactcctc
    16801 atttttattt gattttctga ttcctctcca cttatatata aaactttata accattttta
    16861 gcaagattac cacatacagt taaaagcaat gttgactttc ccactcccgg ttctcctgca
    16921 acaagtatat aacttttttt aacaatgcct cctccaagaa ccctgtcaaa ttcttctatt
    16981 cctgttttta ttcttggtgt ttcttcaaat tcaatttcgg agagtttttt aggatggact
    17041 atttcttttt cttcaaattt ttctagtgat gtataaaatt cttcaataaa tgtattccac
    17101 ttcccgcagt taggacatct accaagtaat ttaacagttt gatatccaca ttcagaacat
    17161 acaaatatac ttttttcttt tttcattttc ttctaaa
//
LOCUS       DAOSAT010000030        17057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2317_length_17057_cov_5.090578, whole genome shotgun sequence.
ACCESSION   DAOSAT010000030 DAOSAT010000000
VERSION     DAOSAT010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17057
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2317_length_17057_cov_5.090578"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2014)
                     /gene="lon"
                     /locus_tag="PLF90_03860"
     CDS             complement(<1..2014)
                     /gene="lon"
                     /locus_tag="PLF90_03860"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOK56452.1"
                     /translation="MAILKKNEIKIRRKNVPALPLRDLIIFPQMVVPLLVGRTKSLNS
                     VEEAIANEGYLVVVFQKDPQIEDPVFDDIYSTGVLTKIVQSLKESTGVMKILVEVLER
                     VKINDFTTTKNYFSVNFDYIKEETETDTEVEAMTRLTLELLEKYLNFNISVPKELYNT
                     ITTLENPSKICDTIAGYLPLKIKDKIKILETIPIKDRLSTLIDILNKEIEVLDVQKKI
                     QDKVIKKIEDTQRQYILTEQLKQIQKELGKEEETPEIAELKEKIKKANMPKNVEEKAI
                     EELNRLSKMMPLSPEATVSRTYIEWLCSLPWSVSTQDNLDIKHAQKILDEDHYGLEKV
                     KERILEYLAVRSRTESLRGPILCFVGPPGVGKTSLGKSIARALGRKFVRISLGGIRDE
                     AEIRGHRRTYIGSLPGRIIQSMRKAGVNNPVFLLDEIDKLGKDFRGDPASALLEVLDP
                     EQNKNFSDHYLEVEFDLSKVLFITTANTEFTIPPPLLDRMEVISLPGYTIWEKKEIAR
                     HFLIPRQMKEHGLTEKNIKFSDEAIFKIIKNYTREAGVRNLEREIANICRKVTKKIVE
                     TGKESSYIITSKNIEKYLGPEKFSSLEIEKENKIGVATGMAWTEYGGEIIFTEVLTMK
                     GKGDLILTGQLGDVMKESARAALSYVRAHAEEFGINPDFYKELDIHI"
     gene            complement(2016..2615)
                     /locus_tag="PLF90_03865"
     CDS             complement(2016..2615)
                     /locus_tag="PLF90_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOK56453.1"
                     /translation="MKNQLIPYVIEVTERGERAYDIYSRLLKDRIIFLGSPITDHIAN
                     IIIAELLFLQNEDPKKDIHLYLNTPGGSITAGLAIYDTMQYVSCDICTYCIGQAASMG
                     AVLLAGGTKGKRFLLPHSRVLIHQPWGGVEGTVVDVSIHTKEMIRLREIINEILALHT
                     GQPIKKIEKDTERDYFMNSEEAINYGLADKIIQSTKLPK"
     gene            complement(2612..3613)
                     /locus_tag="PLF90_03870"
     CDS             complement(2612..3613)
                     /locus_tag="PLF90_03870"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOK56454.1"
                     /translation="MKVDINKKSEIEVELLCEAEKVEIEEEKNKIIKEIKKDAEIEGF
                     RKGKVPEDIIEKKFSETIKENILKNIISKAYYETLKKENFIPVIQPEIYDVEFTEEKL
                     KFKIGLELKPGVKIKKYKGITVKKVEPKEVKEEDVENTLKELEKRPEFASSVIDLEKR
                     QMWKKRIREELEEREKNSARIEEEKQLWNDLFKNAEFPIPEKLVAKRAREYTEYHLRR
                     MNLQGKTREEIENYAQQIFKAVKPYAEEEVKKYFILDKIAEIENVVVEEKEVDERIEI
                     FSRSIGKPFEEVKKELEKTGEIENIRDEIKIDKAYKIVKNNVNYIERIVIPGEENKK"
     gene            complement(3635..3705)
                     /locus_tag="PLF90_03875"
     tRNA            complement(3635..3705)
                     /locus_tag="PLF90_03875"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3671..3673),aa:Gly,seq:tcc)
     gene            complement(3948..4031)
                     /locus_tag="PLF90_03880"
     tRNA            complement(3948..4031)
                     /locus_tag="PLF90_03880"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3995..3997),aa:Tyr,seq:gta)
     gene            complement(4066..4251)
                     /gene="ssrS"
                     /locus_tag="PLF90_03885"
     ncRNA           complement(4066..4251)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLF90_03885"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            4291..4719
                     /gene="tsaE"
                     /locus_tag="PLF90_03890"
     CDS             4291..4719
                     /gene="tsaE"
                     /locus_tag="PLF90_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOK56455.1"
                     /translation="MIFYTKNSKETIKLGEKIAKYLKKGDIVGFIGELGSGKTTMIKG
                     IVKKFSHNSVFSPSFVIVNEYKGKIPVFHFDLYRLESFDELFDIGWTDYLDKGIILIE
                     WAERIKKNLPKKTIYVKIKNVGERKRIIEIKNLKGVKNDK"
     gene            4709..5998
                     /locus_tag="PLF90_03895"
     CDS             4709..5998
                     /locus_tag="PLF90_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK56456.1"
                     /translation="MTSRERILRALNHQIPDRVPIHDSPWGATVRRWHKEGLPEDTTP
                     AEFFGYEFEYFGADTSPRFPVRVIEKNEEYIITTTPQGGKRKNHRDYSTTPEIIDWPV
                     KTKDDWKEIKKLLKPDWTRVDWASGLARNKAAYEEGKFCVFSGGYGYDILQGYMKSEQ
                     LLITMVEDPDWVKEMIMTLAELTLVMADLMIRNGFKFDGAFMFNDMGYRNGLLFSPDT
                     YMKTHYEADKIVYEYFHSKGMKTLLHSCGNVKELIPILIEVGLDCLQPLEVKAGMDLI
                     ELKEKYGDKLSFMGGIDVRLMADPDPSKIEEEIKRKFEVAKKNGGYIYHSDHSIPNTI
                     SFQQYSYVIELVKKYGVYSEYEEIEKHEKEKIVIKEEKIEKPEITEKKEKRGIFGKKA
                     KQKKEEISVEAKKEETEVKKEKKKFSLFGIFRKKKQK"
     gene            6047..7282
                     /locus_tag="PLF90_03900"
     CDS             6047..7282
                     /locus_tag="PLF90_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOK56457.1"
                     /translation="MISIISLFFVFLIIAIRQIGNIKIQIWQAMLGGCLIVLIAHQIS
                     FVNALKSINWEVMFFLFSMFVIGVALEESGCLSYITYRIFRKAKTVNQLLLLILFGIG
                     FSSALLMNDTLAIIGTPVILHIAKKHNINAKFLLITLAFSITTGSVFSPVGNPQNFLI
                     ATEGNLKNPFLTFFHYLFFPTIINLFALFFLLKLFYREHFKNNSLNHYQETIKDNSLA
                     FLSGISIFIFVILIFTKIFLSLTDSKIDLKLVHITVISALPILIFSKKRVLIVKKIDW
                     HTLIFFASMFILMASVWNTGFFQSIIKNLKINITSINMIMIISIILSQLISNVPLVAL
                     YLPLLIQSGAGVKEMMVLASGSTISGNLFILGAASNVIIIQNAEKKYGETITFWEFAR
                     VGIPVTFINAFIYLLFFKI"
     gene            7343..8101
                     /locus_tag="PLF90_03905"
     CDS             7343..8101
                     /locus_tag="PLF90_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOK56458.1"
                     /translation="MKCGVQLYTLRDYCKSVEDTVNTLKKIREMGYKYVQISAVSEPK
                     DVRELKKILDDTELVAVSTHTGYERIIKETEKVIEEHKILGCEGIMCPGLPGELHNKD
                     GYLKVADEFNKIIEKIKKNGLILGYHNHGIEFERYNGKTGLEILLDNCPELEGEIDTY
                     WVTYGGGDPAYWIERYNGRVSQVHFKDMGIIKNQQVMPPIGSGNLNWERIIKSCKKSK
                     VKFCLVEMDRPTCDPFEALKISLENLISFGVKNK"
     gene            complement(8104..9159)
                     /locus_tag="PLF90_03910"
     CDS             complement(8104..9159)
                     /locus_tag="PLF90_03910"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOK56459.1"
                     /translation="MKVNKIKCENFIVYRKENEFSAWPRNCGVYKYKGDEIVVNFFTR
                     TCNYQTKEEVGHGYEPPDNSSRIMQIRSKDGGKNWNNSKKILTPINLGKGETENINFL
                     SPKEFNFKNPDFMLIAIKNNLFFSDDRGKTLYGPCRLPSFGYDFNWARPDYVVREDGA
                     LILFSTVNCSDGKEGKPIAIISKNNGLSWEILSYISSEKNDYMQIMPSGIILPDGKIL
                     CAIRCQRYRHGYSFWSECYISEDSGRSWQFLSRINDIGSPCHLLLLKDGRILATYGYR
                     SYPFGIRCSISEDYGKTWKNEFIIRDDGGSWDLGYPVSIQLDSGKILTFYYFNDKNDK
                     IQVDGGVRYIAGSIFEI"
     gene            complement(9179..12748)
                     /locus_tag="PLF90_03915"
     CDS             complement(9179..12748)
                     /locus_tag="PLF90_03915"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOK56460.1"
                     /translation="MGRKIFLSFLFIYFLIYFSSYCKWVINKELFPVCLDFGDKKTPA
                     AESFIKITQETIYNEKTGYGWEKKYNIYPTGTKDLTSTPNQTGLASTEEANLIINLKN
                     GIYKGIITPAVVGKDIGRLYRYSLLGNEKVLVEKVELPERTFTVKVTDGKLKLTFKPK
                     ENGWIVHSIVLMPIENFLKAPEEFEKTKRLLIVGDEDWILNLQIPELKYKETPFPSFM
                     KEDILIFKKSITDIITPVYRPEEEEILKKIEMKAAGDEYESYQFGIISKKPLPQLKFK
                     ISELKGPKTIPPQNITIRTQYYFNYKKSLTPKTLDLREETELIPNYTQGFWLTVYIPP
                     DTPEGIYKGEISINGLNMKEKKIPVELTVLPFNLPQLKRHFYNIMWLGTYDTKPDSPS
                     QEICKMAILDQKAHGINAFGCELRYPACNIYKEGDEIKFDLKPLEYRINFYKSLGINL
                     DKNGKFIWLIGIHPQKTLAELTGTDTRILDKYVVEPLSFTDKFLEVYSQIVAAVEEYA
                     KKNDLPEMYLFNIDEPYGIYKNKVALQLLKATKKGGGHPWVTVNDTYYQPLKEYLDVP
                     VFANGYASYKTYEEYKKMGKKFWFYSAAPRGLRPVYARYETGIYLYKVGFEGATWFVY
                     TWSLGNAYNGLSSMWNATHPGFWEFVPTISWECTREGIDDVRYMEFLEDLLNKNKEEK
                     IFNVFNSLLDIVDPDINNFLHIDPITDVLVGKDASTWKSRDLDLMREFLQKSTIKLLK
                     GKEGITKENKGRKLKDLKVSFDKEVKEELKTREVDFYSSKYICEALLIEKNLIKIDGE
                     MEDLWKNGYKIENFVKLNEPDNPADDKTEVYILRDKENLYLFFKCFTNDMTKIKATIT
                     GDKRDIKEIWREDGIEIFIDPGCKYLSYYQLMINANGALTDMKVDIGEKKEDDLEWNS
                     NAEVKTKILKDLWTAEIKIPFKSLGNYEIYGINFCRNNTQEKGTHTLWSPTKKASLGN
                     QGFGVPERFGKLLLDKKEIYRKVKSIKGELVFQPLGVENKVELEIDKSLLGKKMDINI
                     VNFKNEKVFEKNILLEKEKIEIPFVIYQDNNDPIYYLKFKCDDYIFEIPFKFLTKDIN
                     FFVFPENAFLIYSSDEKETKLHGSIKINKAEIVKNPDAKLKIVLEYISGGILIEKNLK
                     MISNNFNIWLPIKDLDSGGYILKVEVYINPETVFKNEYKFLKL"
     gene            complement(12772..16242)
                     /locus_tag="PLF90_03920"
     CDS             complement(12772..16242)
                     /locus_tag="PLF90_03920"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56461.1"
                     /translation="MKRFIMFFFVLCFFIFGAEFKSKIYYLEWEQISKELKFNLCLEK
                     EEFSNLDGEGRIVFGIEKGNTINSLNELTLGIRITTLDEGKLIFEKEIPVEKGIVVMD
                     FDLDSLEPNEYQIQAEIKKGNNILKKEEKIFRYVKEKPPLNKGKIPLIFSTDIPLKND
                     TFPVSFGIPFPKGALYDEKNVRIVDKNGKEVPSQKFVRSRWGFSNKSSIRFLGIDFQT
                     EGVKSNWQNKNEVNYYVEFGPTINYDTKSGIKIEETENEYIVDNGAIKFTIKKKGWNL
                     IDSVISGGKEIFKNKKENGFYLIDHEGSIYRASNDSEVKVEIEEKGDIKTVFRIEGWY
                     VKDNSEGKTVNYSLPTDKLCKFITRIEVYKGKPYIRVLHTIIFTFDSNMVRLRDIGFS
                     LPSDFKNAFFGIEGEQAFKIDKNSLKTGSIYLLQHLPDKFSIETSDGRIIKEGRHSEG
                     WFAIEKENGNIIGISNRETWQKFPKEFEVLNDRVNFHIWPSHGKTHSEINEIEPTEIY
                     KLWFCHQGKELNFAMPWKYYFKAAEIYNSPSTGVYTAPGLVLAGVHSSILGAGITNDF
                     LITFLENNIEKQKEISNCFQILPHCIPDPKWTCDSLAVGYIAPYSPEKFKFIEDCIEK
                     IVKSYWELQDYTGQYGMWIYRTWHHTSYKGDKTFDLYRFYNATHHYEAFMPWLLYIRS
                     GDPFYLTQGRANTRLLSDLNIIHYDDPNYNHKEFHFHQKRIIGSTRHTNGFCPWGGDH
                     GLLAHLTCYNALIVAYYFTGDLRLKEVVVDEWQKTIVSNRSHPEFNGAIRLEPGRDPN
                     NALGELIDLYQMTYHPALLIYIDDCLRKFLDNMYHWGEVLHNVLLFYKSERAKKQLLE
                     GVSNPKSDKVNLWTTHAPHENYALASIFETDKEKSKEYAVKSFFSSNFYEWNGKGNKI
                     KNKEPFGAFCSIPDFILYVPRVIYALSKSNIEDPTNWIYSQSLPGCPSGTYCIVKEET
                     DRNFNIIIRGDIGKKESGGFPLKVIDPEKKVIIETLIDKSYTKLTVPKDGKTGEYLII
                     LEVRDGKDDVYVPLTDLEKEVYYVNYWFQCTETKYFTKPKTEEKEIIGIKPHVYSVYI
                     ISNKTKEVIALTEKGEKIEIEMPSEGIWILGKTRYMHLKQPLILSNDEKHWFLPESIK
                     EIQFKWKTTW"
     gene            complement(16239..16940)
                     /locus_tag="PLF90_03925"
     CDS             complement(16239..16940)
                     /locus_tag="PLF90_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56462.1"
                     /translation="MKKLFFIFFLLFEISFSEELHLSSEGFYFYEKEDKEWHINFKGK
                     IPSFCGFYFILFDTKGNIFYSGAIPSSDYSEKSYTIKIPKDNIAGFYKGVIVGEEAVF
                     DSLSLPLSDLEYEVYGGDYFATHYKGRLYFQVDSDDIYILGAYKGNLKILNEKEEVIA
                     DTRITKKQEKYDNLTEFNGKKGITYILEKECRYFRIKNNKKIFLSNDPKKLFIPDPEL
                     DKIEWWKLPLKKETR"
BASE COUNT         5349 a         2850 c         2285 g         6573 t
ORIGIN      
        1 gaatatgaat gtcaagttcc ttatagaaat cagggtttat tccgaattcc tctgcatggg
       61 cacgaacata acttaaagca gcacgggcgg attctttcat aacatctcca agttgtcctg
      121 ttagaattaa atctcctttt cccttcattg ttaaaacttc tgtaaatatt atttctcctc
      181 catattcagt ccatgccatt ccagttgcaa ctcctatttt gttttctttt tctatttcaa
      241 gagatgagaa tttctcaggg ccaagatatt tttctatatt tttggaagtt ataatataag
      301 aactttcttt tcctgtctct acaatctttt ttgttacttt tctgcagata tttgctatct
      361 ctctttcaag atttctgact cctgcttctc ttgtatagtt ttttataatt ttaaatattg
      421 cttcatctga aaattttata tttttttcag taagtccgtg ttccttcatc tgtcttggaa
      481 ttaaaaaatg tcttgcaatt tcttttttct cccatatagt atatccgggt aaagatataa
      541 cttccattct gtcaagcaaa ggagggggaa tagtaaactc tgtatttgca gttgttatga
      601 ataaaacttt tgaaaggtca aactcaactt caagataatg gtctgaaaaa tttttgttct
      661 gttcagggtc aagtacttca agtaatgcac ttgcagggtc ccctctaaag tcctttccta
      721 gtttatcaat ttcatcaagt aaaaaaacag ggttattaac gcctgctttt ctcattgatt
      781 gaattattct tccgggtaat gaaccaatat atgttcttct gtgtcctcta atttctgctt
      841 catcccttat ccctccaaga gatatcctca caaattttct tccaagggct ctggcaattg
      901 actttccaag agatgttttt ccaactccgg gaggacctac aaaacagagt attggacctc
      961 ttaaagattc agttctactt cttactgcaa gatattcaag aattctctct ttaacttttt
     1021 caagtccgta atggtcttca tcaagaattt tttgagcatg ttttatgtca aggttatcct
     1081 gtgtggaaac agaccatggt aaactgcata gccattcaat ataagttctt gaaacagttg
     1141 cttcaggaga taatggcatc atctttgaga gacggttcag ttcttcaatt gctttttctt
     1201 caacattttt cggcatatta gcctttttaa ttttttcctt caattcagca atttccggtg
     1261 tttcttcttc tttcccaagt tctttttgta tctgttttaa ttgttctgtc agaatgtatt
     1321 gtctctgtgt atcttcaatt ttttttatga ctttatcctg aatttttttc tgaacatcaa
     1381 gaacttcaat ctctttattt aatatatcaa ttaatgtact caatctatct ttaatcggta
     1441 ttgtttcaag aatttttatc ttatctttaa tttttaaggg taaataaccc gctattgtat
     1501 cacatatttt tgaaggattt tcaagtgttg taattgtgtt atataattct ttcggtacag
     1561 atatattaaa attcaagtat ttttccagaa gttccagagt aagtcttgtc attgcttcaa
     1621 cttctgtatc tgtttctgtt tcttccttta tataatcaaa attcacagaa aaataatttt
     1681 ttgttgttgt aaaatcattg atttttactc tttcaagaac ttcaacaaga attttcatta
     1741 ctcctgttga ttcctttaaa ctctgcacaa ttttagtgag aacacctgtt gaataaatat
     1801 catcaaaaac agggtcttct atctgtgggt ctttttgaaa aactactaca agatacccct
     1861 catttgcaat tgcttcttca actgagttta atgattttgt tctcccgact aaaaggggta
     1921 caaccatctg agggaaaata ataaggtctc tcaaaggtaa agcaggtaca ttttttcttc
     1981 ttattttaat ttcatttttt ttaagtatag ccattttatt ttggaagttt tgttgattga
     2041 attattttat ctgcaaggcc ataatttatt gcttcctctg aattcataaa gtaatctctt
     2101 tctgtatctt tttcgatttt ttttatgggc tgtcctgtat gcagtgcaag aatttcattt
     2161 attatttctc ttaaccttat catttctttt gtgtgaatac ttacatcaac aacagttcct
     2221 tctactcctc cccatggctg atgaattaaa actctgctat gaggaaggag aaatcttttt
     2281 ccttttgttc cacctgcaag taaaaccgct cccatacttg ctgcctgtcc tatacagtaa
     2341 gtacatatat cacaggaaac atattgcatg gtatcgtata ttgcaagacc tgctgtaata
     2401 gaacctcccg gtgtatttaa atataaatgt atatcctttt tcgggtcttc attttgcaaa
     2461 aataaaagtt ctgcaattat tatatttgca atatggtctg ttattggaga accaagaaat
     2521 attatcctat ccttgagcaa ccttgaataa atgtcataag ccctttcgcc tctttctgtt
     2581 acttcaatta catatgggat aagttgattt ttcatttctt attttcctca ccaggaataa
     2641 caattctttc aatatagttc acattatttt taacaatttt gtatgcttta tcaattttta
     2701 tctcatctct tatgttctca atttctcctg ttttttcaag ttcttttttt acctcttcaa
     2761 atggtttacc aatacttctg ctgaaaattt ctattctttc atcaacttct ttttcttcaa
     2821 caactacatt ttcaatttct gcaattttat caagtataaa atatttttta acttcttctt
     2881 ctgcatatgg ttttactgct ttaaaaatct gttgagcata attttcgatt tcctctcttg
     2941 ttttaccctg caaattcatt ctccttaagt gatattcagt atactctctt gctctttttg
     3001 cgacaagttt ttctggaata ggaaattctg cgtttttgaa aaggtcattc cataattgtt
     3061 tttcttcttc aattctggca ctattttttt ctctttcttc aagttcttct cttattcttt
     3121 ttttccacat ctgtcttttt tcaaggtcaa ttacagaaga agcaaattcc ggtctttttt
     3181 caagttcttt caatgtattt tccacatctt cttcttttac ttctttcggt tcaactttct
     3241 taacagttat tcctttatat ttttttatct taacacctgg ttttaattcc aatccaattt
     3301 taaattttaa cttttcttct gtaaattcaa catcataaat ttcaggttgt atcacaggta
     3361 taaaattttc tttctttaag gtttcataat atgcttttga aatgatattt tttaagatat
     3421 tttctttgat tgtttcactg aatttttttt caattatatc ttcaggcact ttcccttttc
     3481 taaaaccttc aatttcagca tcttttttta tttccttaat tattttattt ttttcttctt
     3541 caatttctac tttttctgct tcacataaca attcaacctc tatttcactt tttttattta
     3601 tatcaacttt cattttttat ctcctttata aaagagcggg gtacgggaat cgaacccgta
     3661 tcctcagctt ggaaggctga tgttttacca ttaaaccaac cccgcttaat tttagaaaaa
     3721 agaataagtt tttcaaagaa catttagttg ggcagggaag gattcggcgc ctccgttcgc
     3781 ttaagaaata tttttctttc gaaaaatgct cgctacggtc gccactcggc actggaattg
     3841 ctccacttgg tcgcaattcc ttaacccgtg cctcacttcg aatccttcca cttatgatgc
     3901 tttttttact tctgtttcaa tcctcagttt ttcaaagaac atttagttgg gcagggaagg
     3961 attcgaacct ccgaaggcgt agagccggca gatttacagt ctgctgccat tgtccactcg
     4021 gccacctgcc ctttatttta taattatacc atattttata agtaataaaa acccgttccg
     4081 gcgtagagat tttacccttt gagacccgta ttttaaagtg ggtgcctccc cggatagttt
     4141 tgacttttcg ccataaggca tggcgaacct gtcagctcca tccggacttg gctccctata
     4201 tatggtcgtt ggctcaaggg ttcttaatct ccgcgtccgg aacaggtatt tatattttac
     4261 ctttttaaaa ataaaaagta aaatcttttt atgatatttt acacaaaaaa ctcaaaagaa
     4321 acaataaaac ttggagaaaa aatagcaaag tatttaaaaa agggagacat tgttggattt
     4381 attggagaac ttggtagcgg gaaaacaact atgataaaag gtattgtaaa aaaattttca
     4441 cataacagtg ttttcagtcc ttcttttgta atagtaaatg agtataaagg caaaattcct
     4501 gtttttcatt ttgaccttta ccgtcttgaa agttttgatg aactttttga tattggctgg
     4561 actgattatc ttgataaagg aataatttta attgaatggg cagaaagaat aaaaaaaaat
     4621 ttgccaaaaa aaacgattta tgtaaaaata aaaaatgttg gggaaagaaa aagaattatt
     4681 gagattaaaa atttaaaagg agtaaaaaat gacaagtaga gaaagaattt taagagcctt
     4741 aaatcatcaa atccctgaca gagttccaat tcatgatagt ccatggggag caactgttag
     4801 aagatggcat aaagaaggat tacccgagga taccacacct gctgaatttt ttggatatga
     4861 gtttgaatat tttggtgctg atacaagtcc aagatttcct gtaagagtaa ttgaaaagaa
     4921 tgaggagtat ataatcacaa caacaccaca gggaggaaaa agaaaaaatc acagggatta
     4981 ttctacaact cctgaaatta ttgactggcc tgtaaaaaca aaggatgact ggaaggaaat
     5041 caaaaaattg cttaagcctg attggacaag agttgactgg gcttctggac ttgcaagaaa
     5101 taaagcagcc tatgaggagg gaaaattctg tgttttcagt ggtggatatg gatatgatat
     5161 acttcagggt tatatgaagt cagagcaact tttaattaca atggttgaag accctgattg
     5221 ggtaaaagaa atgattatga cacttgcaga acttactctt gttatggctg acttgatgat
     5281 aagaaatggt tttaaatttg atggtgcttt tatgtttaat gatatgggat atagaaatgg
     5341 tcttttattt tcacctgata cctatatgaa aacacattat gaggcagata aaattgttta
     5401 tgaatatttt cattcaaagg gtatgaagac attacttcat tcctgtggaa atgttaaaga
     5461 acttatacct attttaattg aagttggact tgattgttta cagccacttg aagttaaagc
     5521 aggaatggat ttaattgaat taaaagaaaa atatggagat aaactttcat ttatgggagg
     5581 tatagatgta agattgatgg cagacccaga tccatcaaaa attgaagagg agataaaaag
     5641 aaaatttgaa gttgcaaaga aaaatggtgg ttatatctat cattcggacc attctattcc
     5701 aaataccata agttttcagc aatactctta tgttatagaa cttgtaaaaa aatacggtgt
     5761 atattctgaa tatgaagaaa ttgaaaaaca tgaaaaagag aaaatagtta taaaagaaga
     5821 aaaaatagaa aaaccagaga ttaccgaaaa gaaagaaaaa agaggaattt ttggaaaaaa
     5881 agcaaaacag aaaaaagaag aaatatctgt tgaagcaaaa aaagaagaaa cagaagttaa
     5941 aaaagaaaag aaaaaatttt ctctattcgg aatattcaga aagaagaaac agaagtaatt
     6001 tttttaaatt tttattttct gctaaaaata cctttttcaa attactatga tttcaataat
     6061 aagtttattt tttgtttttt taattattgc aataagacag ataggaaata taaaaattca
     6121 aatctggcag gcaatgttag gtggctgttt aatagttctt attgcacatc agatttcttt
     6181 tgttaatgct ttaaaatcaa taaactggga agttatgttc tttttattca gtatgtttgt
     6241 tattggagtt gctcttgaag aaagtggctg tctttcctat atcacataca gaatatttag
     6301 aaaagcaaaa acagtaaatc agttattact tttaattcta tttggaattg gtttttcttc
     6361 tgctctattg atgaatgata cccttgccat aataggaact cctgtgatat tacatattgc
     6421 aaaaaagcat aatataaatg ctaaatttct tttaattact cttgcttttt ccataaccac
     6481 tggtagtgtt tttagtcctg ttggtaatcc ccagaacttt cttattgcaa cagaaggaaa
     6541 tctaaaaaat ccatttctta catttttcca ttatctattt tttcctacaa tcatcaatct
     6601 tttcgcttta ttttttttgt taaaactttt ttacagggaa cattttaaaa acaactcatt
     6661 aaatcactat caggagacaa taaaagataa ttctcttgct tttttgtccg ggatttctat
     6721 ttttattttt gttattttaa tattcactaa aatttttctt tctttaactg atagcaaaat
     6781 tgacttaaaa cttgttcata ttactgttat ttctgcactc ccgattttaa ttttcagtaa
     6841 aaaaagggtt ttaatagtta agaagattga ctggcatact ttaatatttt ttgcttcaat
     6901 gttcatattg atggcgagtg tctggaatac aggtttcttt caatcaatca taaaaaatct
     6961 aaaaattaat ataacatcta tcaatatgat aatgattatc agtataattt taagtcaatt
     7021 gatatcaaat gttccacttg tagcacttta tctacctttg cttattcagt caggtgcagg
     7081 tgttaaagaa atgatggttc tcgcttcagg cagtacaata agtggtaatt tatttattct
     7141 gggtgctgca agtaatgtta ttataatcca aaatgctgaa aagaaatatg gagaaacaat
     7201 aactttctgg gaatttgcaa gagtggggat accggtaact tttataaatg cttttatcta
     7261 tcttctattt ttcaaaattt gaaaaagaaa ttttaagtgt taaaatgttt cttaaagttt
     7321 ctgaacgaaa aaggaggaaa aaatgaaatg tggagttcaa ttatatacat taagagatta
     7381 ttgtaagtca gttgaagata cagtaaatac attaaaaaaa atcagagaaa tgggttataa
     7441 atatgtccag atttctgctg tgagtgaacc aaaagatgta agggaactta aaaaaatact
     7501 tgatgataca gaacttgttg ctgtttcaac tcatactggt tatgaaagga ttataaaaga
     7561 aacagaaaag gtaatagagg agcataaaat tcttggatgt gaaggtatta tgtgtccggg
     7621 attgccagga gaattacata ataaagatgg atatttaaaa gttgcggatg aatttaataa
     7681 aataattgaa aaaattaaaa aaaatggact tatacttgga taccacaacc atgggattga
     7741 atttgagaga tataatggta aaacaggact tgaaatttta cttgataatt gccctgaact
     7801 tgagggtgaa atagatactt actgggttac atatggtgga ggagaccctg catactggat
     7861 tgaaagatat aatggaaggg taagtcaggt acattttaag gatatgggaa taataaaaaa
     7921 tcagcaggtt atgccaccta taggaagtgg aaatttaaac tgggaaagga ttataaaatc
     7981 ctgtaagaag tcaaaggtta aattctgtct tgttgaaatg gatagaccta cctgtgaccc
     8041 atttgaagca cttaagataa gtttagaaaa cttaatttct tttggagtta aaaacaaata
     8101 aatctaaatt tcaaaaatag aacctgctat atatctaact ccaccatcta cctgtatttt
     8161 gtcattttta tcattgaaat aatagaaagt taaaattttt ccactatcta attgaatact
     8221 tacaggataa cctaaatccc aggaaccacc atcatctctt ataataaatt cattcttcca
     8281 tgtttttcca taatcttcgc ttattgaaca tcttattcca aaggggtatg acctgtatcc
     8341 ataagtagca agaattcttc catctttaag caataataaa tggcatggac ttccaatatc
     8401 atttattctt gataaaaact gccagcttct accactgtct tctgaaatat aacattctga
     8461 ccagaaactg tatccatgcc tgtatctctg acatcttata gcacataaaa ttttcccatc
     8521 aggaaggata ataccagagg gcattatctg catatagtcg tttttttcag atgatatata
     8581 agaaaggatt tcccaggata aaccattatt ttttgatatt attgctatag gttttccttc
     8641 ttttccatca gaacaattga cagtagaaaa taaaattaaa gcaccatctt ctctgaccac
     8701 ataatcaggt cttgcccagt taaaatcata tccaaaagaa ggtaatctgc atggtccgta
     8761 aagagttttt cctctatcat cagaaaaaaa tagattattt ttgatagcga taagcataaa
     8821 atcaggattt ttaaaattga attcctttgg tgatagaaaa tttatattct ctgtttctcc
     8881 ttttcccagg tttattggag ttaatatttt ttttgaattg ttccagtttt ttccaccatc
     8941 ttttgacctt atctgcatta ttcttgacga attatcaggg ggttcataac catgaccaac
     9001 ttcttctttt gtctgataat tacatgtcct tgtaaaaaaa tttacaacaa tttcatctcc
     9061 tttatattta taaactccac aatttcttgg ccatgcagaa aattcatttt cctttctgta
     9121 aactataaaa ttttcacatt taatcttatt aactttcatc taaatcctcc tttttaattt
     9181 aaagtttcag aaatttatat tcatttttaa aaacagtttc tggatttata tatacttcta
     9241 cttttaaaat atatccgcca gaatccaaat ctttaattgg caaccagata ttaaaattgt
     9301 ttgaaatcat ttttaaattt ttttcaatta agataccacc tgatatatat tctaatacaa
     9361 ttttcaattt tgcatcagga tttttaacta tttctgcttt atttattttt atacttccat
     9421 gcaattttgt ctctttctcg tcagaagaat aaatcaaaaa ggcattttcc gggaaaacaa
     9481 aaaaattaat atcttttgtt aaaaatttaa aaggaatttc aaatatatag tcatcgcatt
     9541 taaattttaa ataataaatc gggtcattat tatcctggta gataacaaaa ggaatttcaa
     9601 ttttttcttt ttctaacagg atattttttt caaaaacttt ttcattcttg aaatttacta
     9661 tgtttatatc catttttttt ccaagcagac ttttatcaat ctccaattca actttatttt
     9721 caacgccaag tggttgaaat acaagttctc cttttattga ttttactttt ctgtatattt
     9781 ctttcttatc cagaagtaat tttccgaatc tttctggaac gccgaatccc tgatttccaa
     9841 gggatgcttt tttggttgga ctccataaag tatgagttcc tttttcttga gtattattcc
     9901 tgcaaaagtt tattccatat atttcgtaat ttccaagaga tttaaaaggt atttttattt
     9961 ctgctgtcca gaggtctttt aatatttttg ttttaacttc tgcattactg ttccattcaa
    10021 ggtcatcttc ttttttttca ccaatatcaa ctttcatatc tgtaagtgct ccgtttgcat
    10081 ttatcataag ttgataatat gatagatatt tacatcctgg gtcaatgaaa atttcaattc
    10141 catcttctct ccatatttcc tttatatctc ttttatctcc tgtaattgtt gcttttattt
    10201 ttgtcatatc gtttgtaaaa catttgaaaa ataaatagag gttttcttta tctcttaaaa
    10261 tataaacttc tgtcttatca tcagcagggt tatcaggttc attgagttta acaaaatttt
    10321 caattttata tccatttttc cataaatctt ccatttctcc atcaattttt ataagatttt
    10381 tttcaattag gagtgcttcg cagatatatt tactggaata aaaatcaact tctcttgttt
    10441 tcaattcttc tttaacttct ttatcaaaac ttactttcaa atcctttaat tttcttcctt
    10501 tattttcttt agtaattcct tccttccctt ttaaaagttt tatagttgat ttttgtaaaa
    10561 attccctcat taaatcaagg tctctgcttt tccatgtgct tgcatctttt ccaacaagaa
    10621 catctgtaat cgggtctatg tgtaaaaagt tatttatatc agggtctaca atatcaagta
    10681 atgaattaaa tacattaaaa attttttctt ctttattttt attcaataaa tcttcaagaa
    10741 attccatata cctgacatca tcaatacctt ctcttgtaca ttcccatgaa attgttggta
    10801 caaactccca gaaaccagga tgagttgcat tccacatact gctaagtcca ttatatgcat
    10861 ttcccaaact ccatgtataa acaaaccatg ttgctccttc aaatcctact ttataaaggt
    10921 aaattcctgt ttcatatctt gcatatacag gtcttaaccc tcttggagca gcgctgtaaa
    10981 accagaattt ttttcccatt tttttgtatt cttcgtaagt tttgtaagaa gcatatccat
    11041 tggcaaaaac aggaacatct aaatattctt taagcggctg gtaataagta tcatttacag
    11101 ttacccacgg atgcccacca cctttttttg ttgcttttaa aagttgtaat gcaaccttat
    11161 ttttatatat accataaggt tcatctatgt tgaaaagata catttcaggt aaatcatttt
    11221 tctttgcata ttcttcaact gctgcaacta tctgtgagta tacctcaaga aatttgtcag
    11281 taaaagaaag tggttcaaca acatacttat caagtattct tgtatccgtt cctgtaagtt
    11341 cagcaagagt tttttgagga tgaattccta ttaaccatat aaatttcccg tttttatcaa
    11401 ggtttattcc aagtgattta taaaaattta ttctgtattc aagtggtttt aaatcaaatt
    11461 taatttcatc tccttcttta tatatgttac atgcaggata tcttaactca catccaaaag
    11521 catttattcc atgtgctttc tggtcaagaa ttgccatttt gcagatttcc tgtgatggtg
    11581 aatctggttt tgtatcatat gtaccaagcc acattatatt ataaaaatgc ctttttaatt
    11641 gtggtagatt aaatggcaaa acagttaatt caactggaat ctttttttct ttcatattta
    11701 atccatttat acttatttct cctttataaa tcccttcagg tgtatcagga ggaatataaa
    11761 ctgttaacca gaatccttga gtataattag gaattaattc tgtttcttct cttaaatcaa
    11821 gtgtttttgg agtaagactt tttttataat taaaatagta ttgcgttctt attgttatat
    11881 tctgtggtgg gattgttttt ggacctttta attcagatat tttaaatttt aactgaggaa
    11941 gtggtttttt tgatataata ccaaactgat aagattcata ttcatctcca gccgctttca
    12001 tctcaatttt ttttaaaatt tcttcctctt ctggcctata aacaggagta ataatgtctg
    12061 taattgattt tttgaaaata agtatatcct ctttcataaa tgagggaaaa ggtgtttctt
    12121 tatattttaa ttcaggtatt tgtaagttca atatccagtc ctcatctcca acaatcaata
    12181 atcttttagt tttttcaaat tcttcaggtg cttttaaaaa attttcaata ggcatcaaaa
    12241 caatactgtg aactatccat ccattttcct ttggtttaaa tgtcaatttt aattttccat
    12301 ctgttacttt tactgtaaaa gttctttcag gcaattctac tttttcaaca agcacttttt
    12361 cattccctaa aagagaatat ctgtataatc ttcctatatc ctttcccaca actgctggtg
    12421 taattattcc tttataaatt ccgtttttaa gatttattat taaatttgct tcttctgttg
    12481 atgcaagacc tgtttggttt ggggtagatg ttaaatcttt tgtccctgtt ggatagatat
    12541 tatatttctt ttcccatcca tatcctgttt tttcattata tattgtttcc tgtgtaatct
    12601 ttataaaact ttcagcagca ggagtttttt tatcaccaaa atcaagacac actggaaata
    12661 actctttatt tatcacccac ttacaataac ttgaaaaata aatcaaaaaa taaataaaca
    12721 aaaaactcaa aaaaatcttt cttcccatct tttcctcctt tcttgttttt attaccatgt
    12781 agttttccac ttaaactgta tttcttttat actttcaggc aaaaaccagt gtttttcatc
    12841 atttgaaaga ataagtggtt gtttaagatg catatacctt gtttttccta aaatccatat
    12901 cccttcagat ggcatttcta tttctatttt ttctcctttt tctgttaaag caattacttc
    12961 tttcgtttta tttgagatta tataaactga atagacatgc ggtttaatac caattatttc
    13021 cttttcttct gtttttggtt ttgtgaagta ttttgtctct gtacattgaa accagtaatt
    13081 aacataataa acttcttttt caaggtctgt cagaggaaca tatacatcat cttttccatc
    13141 tctcacctca agaattatta aatattcacc agtttttcca tcttttggaa cagtaagttt
    13201 tgtataagat ttatctatta aagtttcaat aattaccttt ttttccgggt ctataacttt
    13261 taaagggaat ccaccacttt ctttttttcc aatatctcct cttattatta tgttaaaatt
    13321 cctgtcagtt tcttccttta caatacagta agttccagaa gggcatccag gtaatgattg
    13381 agaataaatc cagtttgtgg ggtcttctat atttgatttt gatagagcat atattaccct
    13441 tggaacataa agaataaaat caggaattga acagaaagca ccaaaaggtt ctttattttt
    13501 aattttattt cctttaccat tccattcata aaagtttgaa ctgaaaaaag attttacagc
    13561 atattcttta cttttttcct tatcagtttc aaatattgaa gcgagtgcat aattttcatg
    13621 tggtgcatga gtggtccaga gatttacttt atcagatttt gggttagata ctccttcaag
    13681 taattgtttt tttgctcttt cacttttata aaataacaaa acattatgaa gaacttctcc
    13741 ccagtgatac atattatcaa gaaatttcct taaacaatca tctatataaa taagtagagc
    13801 aggatgatat gtcatttgat aaaggtctat aagttcacca agtgcattgt tagggtctct
    13861 tcctggctct aatcttattg caccattaaa ttctggatga cttctatttg atacaattgt
    13921 tttctgccat tcatcaacta caacttcttt taatcttaaa tcacctgtaa aataataggc
    13981 aactattaaa gcattataac aggtaagatg ggcaagtaaa ccatggtctc ctccccatgg
    14041 acagaaacca tttgtatgtc ttgttgaacc aataattctt ttttggtgaa aatgaaattc
    14101 tttgtgatta taattagggt catcataatg tataatattt aaatcagaaa gaagacgggt
    14161 atttgctctt ccctgagtta aatagaacgg gtctcctgac cttatataaa gcaaccatgg
    14221 catgaatgct tcataatggt gtgttgcatt gtaaaacctg tataaatcaa atgttttatc
    14281 tcctttataa gatgtatggt gccaggttct atatatccac attccatatt gtcctgtata
    14341 gtcctgtaat tcccaataac tttttactat tttctcaata caatcttcaa tgaatttgaa
    14401 tttttctggt gaataaggcg ctatatatcc aactgctaaa ctatcacatg tccatttagg
    14461 gtcaggtata cagtgaggaa gaatctggaa acaatttgat atctctttct gtttttcaat
    14521 attattctca agaaatgtta taagaaaatc atttgtaatt ccagcaccaa gaatacttga
    14581 atgaactcct gcaagtacaa gaccaggggc tgtataaacc cctgtagatg gactattata
    14641 aatttctgct gctttaaaat aatatttcca tggcattgca aaattcaatt ctttcccttg
    14701 atgacaaaac caaagtttat aaatttcggt cggctcaatc tcatttattt cagaatgtgt
    14761 ctttccatga cttggccata tatgaaaatt aactctgtca tttaaaactt caaattcttt
    14821 tggaaatttt tgccatgtct ctctatttga aattccaatt atattcccat tttctttttc
    14881 aattgcaaac catccttcac tgtgtcttcc ttctttaata attcttccat ctgatgtttc
    14941 tatagaaaat ttatcaggaa gatgttgaag taagtatatt gaacctgttt ttaatgaatt
    15001 tttgtctatt ttaaatgcct gttctccttc aatcccaaaa aaagcatttt taaaatcaga
    15061 aggtaaagaa aatccaatat ctcttaatct caccatattt gaatcaaatg taaagattat
    15121 tgtgtgtaga acccttatat aaggtttccc tttatatact tcaatccttg ttatgaattt
    15181 acagagttta tctgttggta gagaataatt gacagttttt ccttctgaat tatcctttac
    15241 ataccaaccc tctattctaa atactgtttt tatatctcct ttttcttcaa tttcaacttt
    15301 aacttcacta tcattactgg ctctgtatat acttccttca tggtcaatta aataaaatcc
    15361 attctctttt ttatttttaa atatctcctt tccacctgaa attacactat ctattaaatt
    15421 ccatcctttt ttcttaattg taaacttaat tgctccatta tctacaatat attcattttc
    15481 tgtttcttct attttaattc cactctttgt atcataattg attgtaggtc caaattcaac
    15541 ataataatta acttcatttt tattctgcca gttagattta actccttctg tctgaaaatc
    15601 tatcccaaga aatcttatac ttgatttatt ggaaaaaccc catcttgaac gaacaaattt
    15661 ttgagatgga acttctttac catttttatc aacaattcta acatttttct catcatacaa
    15721 agcacctttt ggaaatggaa taccaaaaga aacaggaaaa gtatcatttt ttaatggaat
    15781 atctgttgaa aaaattaaag gaattttacc tttatttaaa ggtggttttt cttttacata
    15841 tctaaaaatt ttttcctctt ttttcagaat attatttcct tttttaattt ctgcctgtat
    15901 ctggtattca tttggctcta aactatcaag gtcaaaatcc atcactacta tccctttttc
    15961 aacaggtatc tctttttcaa atattaattt tccttcatca agtgttgtaa ttctaatacc
    16021 caatgttagt tcatttaaag aatttattgt atttcctttt tctatcccaa aaacaattct
    16081 tccttctcca tccagatttg aaaattcttc cttttcaaga caaagattaa atttcaattc
    16141 tttacttatc tgttcccatt caagataata aatttttgac ttaaactctg caccaaaaat
    16201 gaaaaaacat aaaacaaaga aaaacataat aaatcttttc atcttgtctc cttttttaaa
    16261 ggcaatttcc accattcaat cttatcaagt tcagggtctg gtataaaaag ttttttaggg
    16321 tcattactca aaaaaatttt tttgttattt tttattctga aatacctgca ttccttttct
    16381 aaaatatatg ttataccctt tttcccattg aattctgtca gattatcata tttttcctgt
    16441 ttttttgtta ttcttgtatc agcaattact tcttcttttt catttaaaat ttttaaattc
    16501 cctttataag caccaagaat atatatatca tcactatcaa cctgaaaata taatctccct
    16561 ttataatgag ttgcaaaata atctccacca taaacttcat attcaaggtc acttaaaggt
    16621 aaactcaaag aatcaaaaac cgcttcttct ccaactataa cacccttata aaaaccagca
    16681 atattgtctt ttggaatttt tatagtatat gatttttctg aataatcaga tgaaggaatt
    16741 gctccagaat aaaaaatatt tcctttcgta tcaaaaagaa taaaataaaa accacaaaaa
    16801 gaaggaattt tacccttaaa atttatatgc cattctttat cttctttctc ataaaaataa
    16861 aatccttcac tggaaagatg taattcttct gaaaaagata tttcaaaaag cagaaaaaat
    16921 ataaaaaaca attttttcat tcctttctcc ttttaatata tcaaatcttt ccatgtaagt
    16981 ttccagaaat cacatccatg atttgtatta ccaccatttc catatcctct taatttctca
    17041 aaaatttttg ctcctgg
//
LOCUS       DAOSAT010000031        17045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2320_length_17045_cov_5.255621, whole genome shotgun sequence.
ACCESSION   DAOSAT010000031 DAOSAT010000000
VERSION     DAOSAT010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17045
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2320_length_17045_cov_5.255621"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1902
                     /locus_tag="PLF90_03930"
     CDS             151..1902
                     /locus_tag="PLF90_03930"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOK56463.1"
                     /translation="MKVEMDLLPSQKEIKSLYLEIPLIEKLTPLFHVCTTSLRVNPAG
                     YLPKGDGVIWTSSQFSDGNWYGNFKPYIWLGSEERGICWFADNDKGWIIDWEKQPPCL
                     ILEREKGILKLKVNLIQKPSKIEEIRHIVFGLMATPAKPMPMDWRKILLNQYYPGYKT
                     IVWMGSTYWGCGEIMKEVYPLNKDMSILTKMQEVRVAKGTDTKKFIQGWERRNLSGEL
                     PKDMKDKKSIINLAEISLNMASKKSDYFSVYWEEFHSISQYHPETKIFKYEWGGGYEY
                     TGIYNLAPSYIDFRCWFAAEFIRRGIGLYFDNAYPKRTYDMETSDAYFLPNGKIQPSA
                     NIWRHREYLKRIWILHQQLSPEEGKPIMLVHMTNTHIIPYLDWNQGNIDLEWFYGSRP
                     SQEKYSPDLLRTESIGRQSGNIPFALASISGGTTKLTQDELLKISKTKFGTMVVHEIK
                     TPLETNIEKKLWEILFNFGYGENDCEVYNYWDENYPLSFSDDEVKSILLKRNEEMLLI
                     MCTWNSQPVKVKVNINTKLLGLNPFLVEEVTDEGKTGETYVIENNSFIIPLDGYDVKI
                     LKLKKKKFKHKKGRNNE"
     gene            1895..3265
                     /locus_tag="PLF90_03935"
     CDS             1895..3265
                     /locus_tag="PLF90_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56464.1"
                     /translation="MNKISFAEIKSYLPQNTKFSFKEISSIIEGNIGRIIKKNVSFLN
                     GKFPIGFWNYTDLEKHSKIYNLSEIKKWADAGFTIVHGPDFKPDDKKQKQLMIKILNW
                     CNKYGIKVILWDPRCIVKNNINISKYKKEVKKSIFDFGKLPALFGFFLCDEPGYNLME
                     DVVKSVRIQREIEPKLIPFVNLLPFFKGVEGRIGTTDWIGFLEDYIEKSNTELLSYDC
                     YFQMNPDEEGVKTYFENLRIFREVSLKKGIPFWTTLLCVGHFNYRCPNYDELRWQFNT
                     SLACGANGILWFFYYMRIPHSNYRFSPVNELWELTETYYNLRLIHKNFHQHYRDLFTK
                     LVSRKVFFYPEASGGWSKFNPKESLISQISCMPENHPLLIGEFVDIVGRPYIMLVNNS
                     MKENVYCTITFTGKNIRVFSWDWDGKEYEGKAYSAILINRGKENIRVSHWLAPGQEAV
                     YRVYKK"
     gene            complement(3646..4539)
                     /gene="thiL"
                     /locus_tag="PLF90_03940"
     CDS             complement(3646..4539)
                     /gene="thiL"
                     /locus_tag="PLF90_03940"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOK56465.1"
                     /translation="MNEKEIIEFLKKNFKSKEAVLGIGDDCAVFEYSKDYYLILTTDC
                     LVEDVHFEKEKFNFYQIGKKAMAVNLSDICSMGGLPKFALISLGLPEAEFRIIKGLTE
                     GVKEMSKKYKFEVIGGNLTKAEKIFIDVFMIGIVEKKYLKTRKGARPGNFIYVTGCLG
                     ASQIKKQFNLNPPVFEIRKLVKKYKISGMIDISDGLSSDLIKLVESSKVGFKLYLEKI
                     PVSEDAKNISKNEIESIKHALNDGEDYEVLFTAEKEEKIPEKIDKVKITKIGEILKEK
                     KYIGVYKGKIFEMKDEGFSHF"
     gene            complement(4532..5854)
                     /locus_tag="PLF90_03945"
     CDS             complement(4532..5854)
                     /locus_tag="PLF90_03945"
                     /inference="COORDINATES: protein motif:HMM:NF025065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOK56466.1"
                     /translation="MKIPFDKKFRCSFCKNFHNVGIENVKYGKLKDIENFIKKEVKSV
                     NKIMIICDYNTWKVAGREINERIKKYNLIPVILKPKKEKRITAKYEYVEEIEKNINGV
                     DLILTVGTGSITDLGKLTGNRYKIPVLSFPTAPSMNGYTSPVSAYIKDGVKITVPVSP
                     CKYVYIDLDIISNAPEELIKAGYADSLAKCFANADWKISSIITGEKFCPLPLKIVSEA
                     EKKYINKGRLLLKRDKKVIKNLMEGLNLGGISMIIAGSSSPASGGEHLISHFLDMYAC
                     MKNIEPFAYHGLQVGTGVFISSLIYENLKKLSETEIENMINKNRIDYEKKLKELTFIF
                     PKSRVVLEEIFNKKIKVLNKLREKLSEKWEEIKKKAFSIVYYPDEIKKFLESADCPVH
                     LKEICENKNLIYNSIVLSRFIRERITILDVADEIGILEEITRNYIKNE"
     gene            complement(5851..6564)
                     /locus_tag="PLF90_03950"
     CDS             complement(5851..6564)
                     /locus_tag="PLF90_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013336536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOK56467.1"
                     /translation="MDGKTEIYVGTSGWLYEWNEGKSFDWYVENSNLNSVELNASFYR
                     FPFPNQIKGWANKCKDINFSVKVHRKITHLLKLSPESKDVWNDFKNLFSPLEEKIKFY
                     LFQMPPSFSTDNFERIIGFFKDFKGKEKVAIEFRHKSWFSEEWVKKIEKIGIVFVSVD
                     SPEIKSFIVKTNDTIYLRFHGRTGWYSHNYTEKELEEIIEKIKVLKPKQIYAYFNNNH
                     NMLSNACEFYKLLKNLQEK"
     gene            complement(6554..7639)
                     /locus_tag="PLF90_03955"
     CDS             complement(6554..7639)
                     /locus_tag="PLF90_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK56468.1"
                     /translation="MKKEKKVKIDEKEVNTKNFLKAVLFDYPDWIPVGVGILPAAWFK
                     YKEGLEKILLSHPVIFPNYKKGSFENIRLSPEYKKGKFVDIWGITWENLEEGICGAPV
                     EELAPLKNWNDFENYKFPDPLKYDRYGNQINWEEIRKNLENLKKQGKIAGGGLFHGSM
                     YMQLYYLRGFENFMIDIATEEPNLEILINIVFEYNMKIIEKWIEIGAEMIYFGDDLGL
                     QKSLPISPEKWRKYIKPPYKKMFEKCVENGLIIYLHSDGYILDIIPDLIECGLDMVNP
                     QIRPNTLKGIEKYCKGKIAVNLDLDRQLFPFATKNQIEDHIKEAIDTLALPEGGLSLS
                     VEIGPDVPLEIIETICSTLEKLNCHGW"
     gene            complement(7636..8829)
                     /locus_tag="PLF90_03960"
     CDS             complement(7636..8829)
                     /locus_tag="PLF90_03960"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOK56469.1"
                     /translation="MKIVIAYSGGLDTSVAIRYLKDKYNAEIIAYTSNIGQKIESSEI
                     LEERAKKAGAKKFYYEDLREKFINEYIIPALKAGAIYQEKYPLATSLSRPLIAEGVIR
                     IAKKEKADAVSHGCTGKGNDQVRFELTFKYLAPDIKVIAPLREWEFKSREEEIEYAKE
                     KEIPVSTTKKKPYSIDENLWGISIECGVLEDPYTEPPEDAYQITTSPEKAPGKPEYIE
                     IGFEKGVFVSLNGKKLNQIEIIEKLTKIGAKHGIGRIDMIEDRLVGIKSREIYEAPAA
                     VILHTAHNELEKLVFSRDLYEFKKLVSIKYSQIIYFGQWFSELRKCLDKFVEESQKYV
                     TGKVKLKLYKGNCIVVGRSSPYSLYKEELATYTEKDIFDHKASTGFIEIFGLTSKLEG
                     ERNKL"
     gene            complement(8845..9954)
                     /locus_tag="PLF90_03965"
     CDS             complement(8845..9954)
                     /locus_tag="PLF90_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56470.1"
                     /translation="MKKLLFLILFFSSFCFSKVFIKDEIKIDGSDEVVFVIGENLILS
                     GEFKNELVGIGKSIKGNFKIKGDFIFAALNQEIAPDIENDFYSVGNKIIFNGRTGGTF
                     TCIGRNIILENTEIDGNLRLMGNRITLKNVKIKQKSSIYGKEIKISGVFSDINLHCEK
                     IEVEKGTEIYGDLIYYSDNYPPKELFSNVKGKIIWKPSYGGKVKQKTETFKKIKFLYS
                     FFSLLFPYFLLLFFTPNLLKSTTLTSGKSFIKSFFLGLFSIIIFSFFILIFLITIIGV
                     PTGLILITFFASALYLSRGFIFIYLGRTILYKLKDNRIIWTLSIFLGILIFNLLSLNS
                     TLKIISNIISAPVGFGALLIDRVKLLKKLKEEKII"
     gene            complement(9951..10424)
                     /locus_tag="PLF90_03970"
     CDS             complement(9951..10424)
                     /locus_tag="PLF90_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOK56471.1"
                     /translation="MKNEEIIIRKSKLKDVPSIRQIVNKFAMEGKMLPLSLNQIYERI
                     RDFTVLERNGKIIGCGALKIIWKDLAEIRSLAVKQQFQKKGYGKKIVEVLLKEAEELE
                     IKRVFVLTYNPEFFSRFGFEKISKQKLPHKIWVDCINCPKFPRCDEIPMMNILKK"
     gene            complement(10421..11029)
                     /gene="purN"
                     /locus_tag="PLF90_03975"
     CDS             complement(10421..11029)
                     /gene="purN"
                     /locus_tag="PLF90_03975"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOK56472.1"
                     /translation="MFTIGVLASGKGTNLQAIIDYIKEENLQIKIGVVISDNPQAYAL
                     EIAKKDGIKSICIPCTKYKTILDKETEIKYVEVLKENNVDLVCLAGFMRVLKSTFIRN
                     FPNRIINIHPSLLPSFPGLESWRQALEYGVKFTGCTVHFVDEGIDSGPIILQAVVPIL
                     PEDTPETLHKRIQEKEHIIYPLAIRLIAEGKVSIVGRKVYIK"
     gene            complement(11017..11325)
                     /locus_tag="PLF90_03980"
     CDS             complement(11017..11325)
                     /locus_tag="PLF90_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOK56473.1"
                     /translation="MKGKFYYIKDVSKLLDIPSHILRYWEKELNFKIMRDKKGNRIYT
                     ENDIEMLKNVKLMIYRDGFKIRGVKKKLKEKKLENKKEYVKLLNKILKKIKEIKKCLQ
                     "
     gene            complement(11322..12308)
                     /locus_tag="PLF90_03985"
     CDS             complement(11322..12308)
                     /locus_tag="PLF90_03985"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOK56474.1"
                     /translation="MGKKIGILGSGGWGISLALLLNKLKNKVFLWEPLKENYEILIKK
                     RENINYFKGIRIPKIIEISNSLEETVLNSEILIIVVRSSFFRDTIKKLKLYYQNQPIL
                     IGTKGLELNTGKRMSEILKEFIKNDSYAVLSGPTIAKEVVKGYPTAAVVASNNKNISE
                     FFQRIISCENFRIYSSKDIIGVEVGGAFKNIIAIGAGIIDGLKLGINTKSSYLTRGLN
                     EMIKIGTFLGGEEKTFRGLSGMGDLITTSFSKYSRNRSFGEGIVKIGKEKYLKNAKMV
                     IEGIPTTRAFYKLSKKLDIELPITEAIYRIIYKNSDVRKEIKKLMLRELKKE"
     gene            complement(12293..12892)
                     /gene="plsY"
                     /locus_tag="PLF90_03990"
     CDS             complement(12293..12892)
                     /gene="plsY"
                     /locus_tag="PLF90_03990"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005892661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOK56475.1"
                     /translation="MNKFYYLVFSYFAGSIPFGYLLCKLFKGIDIRKFGSGNIGATNV
                     YRVCGWKLGIPALILDILKGFIPVYTGKILMFSSPFILASGILSILGHSFSIFLKGKG
                     GKGVSTSFGVVIGLLPLPALISFFVWIIVVLSTKYVSLGSITGALSLTIFTFIFNKNS
                     FLFYVSIFIFIFIVYTHKENIKRLLNKKENRIVFPWERK"
     gene            complement(12894..13532)
                     /locus_tag="PLF90_03995"
     CDS             complement(12894..13532)
                     /locus_tag="PLF90_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOK56476.1"
                     /translation="MANDLLNGIKESVIKGDMKKTEELVDKAIKESLPVEKILNEGLI
                     AGMMVVGEKFKNNEYYVPEVLIAARAMNKGMSLLEPLIVQAGIKPIAKLAIGTVKGDL
                     HDIGKNLVVMMWKGAGFEVLDLGIDVPPEKFVEAAKSGVQVIGMSALLTTTMVQMKET
                     IEVLKKEGVRDNVKIIIGGAPVTQEFANEIGADGYAPDAASAVDKVKELINL"
     gene            13659..13731
                     /locus_tag="PLF90_04000"
     tRNA            13659..13731
                     /locus_tag="PLF90_04000"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13692..13694,aa:Val,seq:tac)
     gene            13814..15700
                     /gene="dnaK"
                     /locus_tag="PLF90_04005"
     CDS             13814..15700
                     /gene="dnaK"
                     /locus_tag="PLF90_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOK56477.1"
                     /translation="MAEKIIGIDLGTSNSSAAIMEGDKPVIIPSAEGTTIVGGKAFPS
                     YVAFTKDGQVLVGEPARRQAAVNPEGTVFAAKRKMGTDFKYHIYGKTYTPEQISAFIL
                     QKIKKDAENFLGYPIKKAVITVPAYFNDNQRQATKDAGTIAGLEVVRLINEPTAASLA
                     YGLDKLKEELKILVFDFGAGTLDVTIMEMMGGVFKVLSTSGDTQLGGTDMDNALIEYI
                     VEQFKRETGIDLRNDKMAMMRLKEAAEKAKIELSSVVETEINLPFITADQTGPKHLIM
                     KINRAKLEELISPIIDRCKGPVDRAIEDAKLKRSEINKVILVGGPTRMPIVQKFIQDY
                     IGIQPERGVDPMECVSIGAAIQGAILAGEMKDKDILLLDVTPLSLGVETLGGVFTKII
                     ERNTTIPVKRSQIFTTATDNQTSVEVHVLQGERPMAKDNLSLGRFHLDGIPPAPRGIP
                     QIEVTFDIDANGILHVSAKDLGTGKEQSIRITGSKKLSQEEIEKMVKEAERFAEEDKK
                     LREIAELSNQADSLIYTVNKTLKEHGNKLSESERKEIEEKINNLRKIIDSETKTADQI
                     RKGIEELETASHKLAQILYQQAQQSYGSAQSQGPQSGQEGKQENEKVVDAEYKVEDDK
                     NKEQ"
     gene            complement(15726..16334)
                     /locus_tag="PLF90_04010"
     CDS             complement(15726..16334)
                     /locus_tag="PLF90_04010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOK56478.1"
                     /translation="MKIKVNPTRMELLKLKKRIAIAKRGHKLLKDKEEQLLIEFRKLI
                     SIVKKERKLIENEFINFCEKVLILRGSIDEKRWNSLLKIPFIEIKYSLNRKRIFNIPV
                     NEIETYISGEFVFNYFLSPYENFMIKEGMRILKKLFILYGLENKLISFAEEIERTRRR
                     VNALEYVLIPNIEEAIRYIQIKLDEYERTSLTTLKHLKLIEG"
     gene            complement(16336..>17045)
                     /locus_tag="PLF90_04015"
     CDS             complement(16336..>17045)
                     /locus_tag="PLF90_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOK56479.1"
                     /translation="ALTAAEYLAFELGMHVLVILTDMTNYAEALREISAARDEIPGRR
                     GYPSYLYTDLATIYERAGRIKGKPGSITQIPILTMPQDDKTHPIPDLTGYITEGQIIL
                     SRALHLKGIYPPVDILTSLSRLRDKGIGIKKETGEKLTREDHPYLASQLFASYARGKE
                     AEELAMVLGEAALTESDIAHLNFARKFEEEFINQGLNENRSIEETLNLGWKLIKMLPK
                     IDIKRIPPFIMEKFWKD"
BASE COUNT         5765 a         2659 c         2307 g         6314 t
ORIGIN      
        1 tctcctatgg tatttatttg agatggagag aaattagaag gaaagggtaa attcacaaaa
       61 ataagtgaaa gttatgtaat ttatgaagga gaaactgtca attctcctat aacaattaaa
      121 actaaatgta caactgaata tgatggatgt atgaaggtag aaatggattt attaccaagt
      181 cagaaagaaa taaaatccct atatttagag attcctttaa ttgagaaatt aacacctttg
      241 tttcatgtgt gtacaacttc tttaagagta aatcctgcag gatatttacc taagggagat
      301 ggggttatat ggacaagttc tcaattttct gatggaaact ggtacggaaa ttttaagccc
      361 tatatttggt tagggagtga agaaagaggt atctgttggt ttgctgataa tgataaagga
      421 tggataattg actgggaaaa acagccacca tgtcttattt tagaaagaga aaaaggaata
      481 ttaaaattaa aagtaaattt aattcaaaag ccctcaaaaa ttgaagaaat aagacatata
      541 gtatttggac ttatggctac tcctgctaaa cctatgccta tggattggag aaaaatttta
      601 cttaatcagt attatccggg ttataaaaca attgtatgga tgggttctac ttattgggga
      661 tgtggagaaa ttatgaaaga agtttatcca ttaaataaag atatgagtat tttgacaaaa
      721 atgcaggaag taagagtggc aaaaggtaca gataccaaaa aatttattca gggatgggag
      781 agaaggaatc tctcaggtga attacctaag gacatgaagg acaaaaaatc tatcattaat
      841 cttgccgaga tttctttaaa tatggcttct aaaaaatcag attatttcag tgtttactgg
      901 gaagagtttc atagtatatc acaatatcat ccagagacaa aaatattcaa atatgaatgg
      961 ggtggtggtt atgagtatac gggaatatat aatcttgcac caagttatat agatttcaga
     1021 tgttggtttg ctgctgaatt tataagaaga ggaattggat tatattttga caatgcatat
     1081 cctaaaagaa catatgatat ggaaacttca gatgcctatt ttttgccaaa cggaaaaatt
     1141 caaccatccg caaatatttg gagacatcgg gaatatctca aaagaatatg gattttacat
     1201 caacagcttt ctcctgaaga aggaaaaccc ataatgcttg tccatatgac aaatacacat
     1261 ataattccat accttgactg gaatcagggt aatattgacc ttgagtggtt ttatggatca
     1321 agaccatccc aagaaaaata ttctccagat ttattgagaa cagaaagtat aggaagacaa
     1381 tctggaaata ttccatttgc tcttgcttct attagtggag ggactacaaa attaactcaa
     1441 gatgaattgt taaaaataag taaaacaaaa tttggaacta tggtagtaca tgaaattaaa
     1501 acaccattag aaacaaacat agaaaaaaag ttatgggaaa ttctatttaa ttttggatat
     1561 ggagaaaatg actgtgaagt ttataattat tgggatgaaa attatcccct ttcttttagt
     1621 gatgatgaag ttaaatcaat tttattaaaa agaaatgaag aaatgttatt aattatgtgt
     1681 acttggaact ctcaaccagt aaaagtaaaa gtaaatatta atacaaaatt attgggctta
     1741 aatccatttc ttgtagaaga agtaacagat gaagggaaaa caggagaaac atatgttata
     1801 gaaaataatt ctttcattat tcctttggat ggatatgatg taaaaatatt aaaattaaag
     1861 aaaaaaaaat ttaaacataa aaaggggaga aataatgaat aaaatttctt ttgcagaaat
     1921 aaaatcttat cttccccaaa atacaaaatt ttcttttaaa gaaatatcat ctattattga
     1981 gggaaacatt gggaggatta tcaaaaaaaa tgtttcattt cttaacggta aatttccaat
     2041 agggttttgg aattatacag atttagaaaa acatagtaaa atttataatc tctctgagat
     2101 taaaaaatgg gctgatgcag gttttacaat agttcatggt cccgatttta aaccagatga
     2161 taaaaaacag aaacaactta tgattaaaat tttaaattgg tgtaataaat atggtataaa
     2221 ggtaattttg tgggacccaa gatgtatagt aaaaaataac ataaatatat ctaaatacaa
     2281 aaaagaagtc aaaaaaagca tttttgattt tggtaaactt cctgcattat ttggattttt
     2341 tctatgtgat gaacctggct ataatttaat ggaagatgta gtcaaatctg ttagaatcca
     2401 aagagaaatt gaaccaaaat taattccatt tgttaatctt ttaccttttt ttaaaggcgt
     2461 ggaaggaaga ataggaacaa ctgattggat tggatttttg gaagattata ttgaaaaaag
     2521 taatactgaa ttattaagtt atgattgtta ttttcaaatg aatcctgatg aagaaggggt
     2581 aaaaacttat tttgaaaatt taagaatatt tagagaagta tctttaaaaa aaggtattcc
     2641 tttttggaca acacttttat gtgtcggaca ttttaactat cgctgtccaa attacgatga
     2701 attaagatgg caatttaata catcattagc ttgtggtgca aatggaattt tatggttttt
     2761 ttattatatg aggattccgc attcaaatta tagattttca ccagtaaatg aactttggga
     2821 attaacagaa acttattata atttgagatt aattcataaa aattttcatc aacattatag
     2881 agatttgttt acaaaactag tatccagaaa ggtgtttttt tatccagaag caagtggggg
     2941 ttggagtaaa tttaatccaa aagaatcttt aatttcgcaa atatcttgta tgcctgaaaa
     3001 tcatccatta ttaataggag aatttgttga tattgttgga agaccttata ttatgcttgt
     3061 aaataatagt atgaaagaaa atgtatattg tacaataact tttactggga aaaatatccg
     3121 tgttttttca tgggattggg atggaaaaga atatgaaggg aaggcatatt ctgcgatact
     3181 aataaataga gggaaagaaa atattagagt ttctcactgg ttagctccgg gtcaagaagc
     3241 agtttacagg gtctataaaa agtgatatta aagagaaatt agtgtgattt tcagtgaaaa
     3301 atttaatttt gaaatagaat ttaaacaaaa ttttttaaaa attttattaa gttgtaaatt
     3361 agatggggaa gtaagattat taagaaggag attttaagat taatttgaaa gaaaaatttt
     3421 gaaagaaaga aaaataaaag agaaattaag aaggtggaaa agtcaagtat gagtggctct
     3481 ggttttcctg gataaaggtt gaattttctt aaaatatagc caataaaaaa acaaaaagaa
     3541 aggattaaat aaaggaaagg tggttgatat ttcctaaaaa tagtaattac taaaaagtaa
     3601 attaaaagcc acttcaaaat tataaccgaa ccgggttcgg aaaatttaaa aatgggaaaa
     3661 accttcatct ttcatttcaa atattttacc tttataaact cctatatatt ttttttcctt
     3721 taatatctct ccaatttttg taatttttac tttatcaatt ttttctggta ttttctcttc
     3781 tttttcagca gtaaataaaa cctcatagtc ctcaccatca tttaaagcat gttttatact
     3841 ttcaatctca tttttactta tattttttgc atcttctgat acaggtattt tttcaagata
     3901 taatttaaat ccaactttac tgctttctac aagtttgatt aaatcagaag aaagaccatc
     3961 tgaaatatca atcatacctg aaattttata ttttttaacc aattttctta tttcaaaaac
     4021 agggggattc agattaaact gtttttttat ttgggaagca ccaaggcaac ctgttacata
     4081 tataaaattt ccgggtctgg ctccttttct tgtttttaaa tatttcttct caacaatacc
     4141 aatcataaaa acatctataa aaattttctc tgcttttgta agatttcctc cgatcacttc
     4201 aaatttatat ttcttactca tttctttaac tccttctgtc agtcctttga ttatcctaaa
     4261 ttctgcttct ggtaaaccca gagaaattaa agcaaatttt ggaagtccac ccatagaaca
     4321 tatatcgctt aaatttactg ccattgcttt tttcccaatc tgatagaaat taaatttttc
     4381 tttttcaaaa tgtacatcct caacaagaca gtcagttgtt aaaatcaaat aataatcttt
     4441 tgaatattca aaaactgcac aatcatctcc aatccctaaa accgcctctt tacttttaaa
     4501 attttttttc agaaactcaa ttatttcttt ttcattcatt ttttatataa tttctggtta
     4561 tttcttctaa aattccaatt tcatctgcaa catcaagaat tgtaattctt tcccttataa
     4621 atcttgaaag aacaatagaa ttataaatta agtttttatt ttcacatatt tctttcaaat
     4681 gaacaggaca gtcagcactt tcaaggaatt tttttatttc atctgggtaa tagacaattg
     4741 aaaatgcctt ttttttaatt tcttcccatt tttctgataa tttttctctc aatttattca
     4801 ggacttttat ttttttattg aatatttctt ctaaaactac tctgcttttt ggaaatataa
     4861 aagtcaattc ttttaatttt ttttcataat caatcctatt tttatttatc atattttcaa
     4921 tttcggtttc acttaatttc ttcaaatttt catatatcag agaacttata aaaacacctg
     4981 taccaacctg taatccatga taggcaaatg gttcaatatt tttcatacag gcatacatat
     5041 caagaaaatg agagattaga tgttctccac cagatgcagg agaggaactg cccgcaataa
     5101 tcattgaaat accaccaaga tttaatcctt ccatcagatt ttttataact tttttatctc
     5161 tttttaataa taaccttccc ttatttatat actttttttc tgcttcactt acaattttta
     5221 atggcaatgg acagaatttt tcacctgtta ttattgaaga tattttccag tcagcatttg
     5281 caaaacattt tgcaagtgaa tcagcatatc ctgcttttat aagttcttct ggtgcatttg
     5341 agattatatc taaatcaata taaacatatt tacaaggaga aactggaact gttattttta
     5401 ctccatcttt tatatatgca gaaacaggag aagtatatcc attcattgaa ggtgcagttg
     5461 gaaaagagag aacaggtatt ttatacctat ttccagtcag tttccccaaa tctgtaatac
     5521 ttcctgttcc aaccgttaag attaaatcaa ctccattaat atttttttca atttcttcca
     5581 catattcata ttttgcagtt attctttttt cttttttagg ttttagaatt acaggtatta
     5641 aattgtattt ctttattctt tcatttattt ctctaccagc aaccttccat gtattataat
     5701 cacaaataat cataatttta tttacacttt tcacctcttt tttaataaaa ttctcaatat
     5761 cttttaattt tccgtatttt acattttcaa ttcctacatt atgaaaattc ttgcagaaag
     5821 aacatctgaa ttttttatca aaaggtattt tcatttttct tgtaagtttt taagaagttt
     5881 ataaaattca caagcatttg aaagcatatt gtgattgtta ttgaagtaag cataaatttg
     5941 ttttggtttg agtactttta ttttttctat tatttcttcc aattcttttt cagtataatt
     6001 atgggaatac catccagttc ttccatgaaa tcttaaataa atggtatcgt ttgtttttac
     6061 aataaacgat tttatttcag gactgtcaac acttacaaaa acaataccaa tcttttcaat
     6121 ttttttaacc cattcttctg aaaaccaact tttatgcctg aattcaatcg caactttttc
     6181 ttttcccttg aagtctttaa aaaatcctat tattctttca aaattatcag tggaaaaaga
     6241 tggaggcatc tggaataaat aaaattttat tttttcctca agtggagaaa ataaattttt
     6301 aaaatcattc catacatctt tgctttcagg acttaatttc aataaatgtg ttatttttct
     6361 atgaactttt actgaaaaat ttatatcttt acatttgtta gcccatcctt ttatctgatt
     6421 tggaaatggg aatctgtaaa aggaagcatt tagttcaact gaatttaaat tagaattttc
     6481 aacataccag tcaaaacttt taccttcatt ccattcataa agccagccac ttgttcctac
     6541 ataaatttcc gttttaccat ccatgacagt ttaatttttc cagggtacta catattgttt
     6601 caattatttc aaggggtaca tccggtccta tttccacact taaagatagt cctccttcag
     6661 gtaaggcaag tgtatcaatt gcttccttga tatggtcttc tatttgattt tttgtagcaa
     6721 atggaaataa ctgtctgtca aggtcaagat ttaccgctat tttcccctta caatactttt
     6781 caataccctt taatgtattt ggtcttatct gtggattgac catatcaagt ccacattcaa
     6841 tcaaatcagg aattatatca agaatgtatc catctgaatg taaataaatt ataagaccat
     6901 tttcaacaca tttttcaaac atttttttat aaggaggttt tatatatttt ctccattttt
     6961 ctggacttat aggtaatgac ttttgaagac caaggtcatc tccaaaataa atcatctctg
     7021 caccaatttc tatccatttt tcaataattt tcatattata ttcaaaaaca atattaatta
     7081 atatttctaa atttggctct tctgttgcta tatcaatcat aaaattttca aatcctctta
     7141 aataataaag ttgcatatac atagaaccat gaaataaacc acctcctgca atttttccct
     7201 gtttttttaa attttcaaga tttttcctga tttcttccca gtttatctga tttccgtatc
     7261 tatcatattt taaagggtct ggaaacttat aattttcaaa atcattccag ttttttaatg
     7321 gagcaagttc ttcaacaggt gcaccacaga tcccttcctc taaattttcc cacgttattc
     7381 cccagatatc tacaaatttg ccctttttat attcaggaga taatcttata ttttcaaaac
     7441 ttcctttttt ataatttgga aatataacag gatgtgaaag caaaattttt tcaagtccct
     7501 ctttatattt aaaccaggca gcaggtaaaa ttccaacacc aacaggtatc cagtcaggat
     7561 aatcaaaaag tactgctttt aaaaaatttt ttgtattaac ttctttttca tcaattttaa
     7621 cctttttctc ttttttcata atttattcct ttcaccttca agtttacttg ttagtccaaa
     7681 aatttcaata aaacctgttg atgctttgtg gtcaaatata tccttttctg tatatgtagc
     7741 aagttcttct ttataaagag agtagggtga acttctgcca actacaatac aatttccttt
     7801 gtaaagtttc aattttactt ttccagttac atatttctga ctttcttcaa caaatttatc
     7861 aagacatttt ctcaattcag aaaaccactg accaaaataa ataatctgtg aatattttat
     7921 tgatacaagt tttttaaatt catataaatc tcttgaaaat acaagttttt caagttcatt
     7981 atgtgctgta tgtaaaatta ctgctgctgg tgcttcatat atttctcttg attttatacc
     8041 taccagtcta tcttcaatca tatcaattct tcctattcca tgctttgctc caatttttgt
     8101 cagtttttct attatctcta tctgatttaa ttttttccca ttcagagaaa caaaaacacc
     8161 tttttcaaat ccaatttcaa tatattccgg ttttcccggt gctttttcag gagaagttgt
     8221 tatctgatat gcatcttcgg gtggttctgt ataagggtct tcaagaactc cacactcaat
     8281 agatattccc cataagtttt catcaatact atatggtttt ttctttgtag tagaaaccgg
     8341 aatttctttt tcttttgcat attcaatttc ttcttctctt gatttaaatt cccattccct
     8401 taatggtgca ataactttta tatctggagc aagatattta aaagtcaact caaatcttac
     8461 ctggtcattt ccctttcctg tacatccatg agatactgca tctgcttttt ctttttttgc
     8521 aattcttata actccttctg ctattaatgg tcttgataga gatgttgcaa gtggatattt
     8581 ctcctgatag attgctcctg ctttaagagc aggtattata tactcattaa taaacttttc
     8641 tcttaagtcc tcataataaa atttttttgc acctgctttt tttgctcttt cttcaagtat
     8701 ttctgatgat tctattttct gtccgatgtt tgaagtgtaa gcaattattt cagcattata
     8761 cttatccttt aaatatctta tagcgacaga agtatcaagt cctccagaat atgcaatcac
     8821 tatcttcatt tttttacctc ctccttaaat tattttttct tctttaagtt tttttaataa
     8881 ttttactcta tctattaata aagcaccaaa tcctactggc gctgaaatta tattacttat
     8941 tattttcaaa gtagaattca gggataaaag attaaaaatt aaaattccta aaaaaattga
     9001 tagagtccat ataattctgt tatcttttaa tttatataaa atggttcttc caagataaat
     9061 gaatataaaa cctcttgata gataaagtgc cgaagcaaaa aatgtaatta gtattagacc
     9121 tgtcggaacc cctataatag taataagaaa aattaaaata aaaaaactga aaattatgat
     9181 tgaaaataaa cctaaaaaaa atgattttat aaaacttttt ccagatgtta aagttgttga
     9241 tttaagtaaa ttaggggtga aaaataaaag taaaaaataa gggaaaagta gtgagaaaaa
     9301 agaatataaa aattttattt ttttaaaagt ttctgttttt tgttttactt ttccaccata
     9361 ggatggtttc catataattt taccttttac attggaaaaa agttctttag gaggataatt
     9421 atcagaataa tatattaagt caccgtaaat ttctgttccc ttttcaactt caattttttc
     9481 acagtgtaaa ttaatatcag aaaaaactcc ggatattttt atttcttttc cataaataga
     9541 acttttttgt ttaattttta cattttttaa agtaattcta tttcccatta accttaaatt
     9601 accatcaatc tctgtatttt ctaaaattat atttctacct atacatgtga aagttcctcc
     9661 tgttctgcca ttaaagatta ttttatttcc gacagagtaa aagtcatttt caatatcagg
     9721 agcgatttcc tgatttaaag cagcaaaaat aaaatcaccc tttattttaa aatttccttt
     9781 tatacttttt ccaattccaa caagttcgtt tttaaattca cctgataaaa taagattttc
     9841 tccgattaca aaaactactt catcagaacc atctattttt atttcatctt ttataaaaac
     9901 ttttgaaaaa caaaaacttg aaaaaaataa aattaaaaaa aggagttttt tcattttttt
     9961 aatatattca tcattggaat ttcatcacac cttgggaatt tcggacagtt tatacaatcc
    10021 acccatattt tgtgaggaag tttctgtttt gaaatttttt caaatccaaa cctgctgaaa
    10081 aattcagggt tataggttag aacaaaaact cttttaattt caagttcttc tgcttctttt
    10141 aataaaactt caacaatttt tttcccgtat cccttttttt gaaactgttg ttttactgca
    10201 agagacctta tttcagcaag gtctttccat attattttta aggcaccaca cccaataatt
    10261 tttccatttc tctccagtac agtaaaatct cttattcttt cataaatttg atttagtgaa
    10321 agaggcagca tttttccttc cattgcgaat ttatttacta tctgtcttat ggatggtaca
    10381 tcttttaact tactttttct gataataatt tcttcgtttt tcattttata taaacttttc
    10441 tcccaacaat acttactttt ccctctgcga ttaatcttat tgcaagagga tatataatgt
    10501 gttctttttc ctgaattctt ttatgtagtg tttcaggtgt gtcttccgga aggataggga
    10561 caactgcttg aagtattatt ggtcctgaat caataccttc gtcaacaaaa tggactgtac
    10621 aacctgtaaa ttttactcca tattcaagtg cctgacgcca actttcaaga ccgggaaaag
    10681 atggtaaaag agatggatgg atatttataa ttctgtttgg aaaatttctt ataaatgtac
    10741 ttttcaatac tcgcataaat ccagcaagac agacaaggtc tacgttgttt tcttttaata
    10801 cttcaacata tttaatttca gtttctttat caagtattgt tttatatttg gtacagggta
    10861 tacagatgct ttttattcca tcttttttgg ctatttcaag tgcatatgcc tggggattgt
    10921 cacttatcac aaccccgatt tttatctgta aattttcctc ttttatataa tcaattattg
    10981 cctgaagatt tgtccctttt ccagaagcaa gtactcctat tgtaaacatt tttttatttc
    11041 ctttattttt tttaagattt tatttaataa tttaacatat tcttttttat tttccaattt
    11101 tttctctttc agttttttct ttactcctct aatcttaaaa ccatctctat aaatcattaa
    11161 cttcacattt ttcaacattt caatatcatt ttctgtataa attctattgc cttttttgtc
    11221 cctcattatt ttaaagttta attccttttc ccagtatctc aatatatgag agggaatatc
    11281 cagtaatttt gatacatcct ttatgtaata aaatttacct ttcattcttt tttaagttcc
    11341 cttagcataa gtttttttat ttcttttctt acatcactgt ttttataaat aatcctgtaa
    11401 attgcttctg taattggaag ttcaatatca agttttttgc tcaatttata aaatgctctt
    11461 gtagttggga ttccttcaat taccattttt gcatttttta agtatttttc ttttcctatc
    11521 tttacaatcc cttctccaaa tgacctgttt ctactatatt ttgaaaatga agtggttatc
    11581 aaatcaccca tacctgaaag tcctctaaat gttttttctt cccctccaag aaaagtacca
    11641 atttttatca tttcatttaa acctcttgta agataagatg attttgtgtt aattccgagt
    11701 ttaagaccat caattattcc tgctcctata gcaataatat ttttaaatgc tcctccaact
    11761 tcaactccta ttatatcctt acttgaataa attctgaagt tttcacaact tattatccgc
    11821 tgaaaaaatt ccgaaatatt tttattatta gaagcaacaa cagcagcagt gggataacct
    11881 tttactactt cttttgctat tgtgggaccc gaaagtactg cataactatc atttttaata
    11941 aactctttca agatttctga cattcttttc cctgtgttta attccagtcc ttttgtccca
    12001 attaatatag gttgattttg ataatataat tttagttttt ttattgtatc cctgaaaaaa
    12061 gatgacctta caactataat taaaatttca gaatttaaaa cagtttcttc taatgaattt
    12121 gatatttcta ttatttttgg aattcttatg cctttgaaat agttaatatt ctctcttttt
    12181 ttaattaaaa tttcataatt ttcttttaat ggttcccaga gaaaaacttt attttttagt
    12241 ttattaagaa gaagagcaag tgaaattccc cagccaccac ttccaagtat tcctattttc
    12301 tttcccatgg aaaaacaatc ctgttttctt ttttatttaa aagtcgttta atattttcct
    12361 tgtgtgtata aactataaag ataaagataa aaatactcac ataaaaaagg aaactatttt
    12421 tgttaaatat aaaagtaaaa attgtcaaag aaagtgctcc tgtaattgaa ccaagagaaa
    12481 catattttgt agaaagaact acgattatcc agacaaaaaa tgagattaaa gcaggaagag
    12541 gtaatagtcc tataacaaca ccaaaacttg ttgagacacc ttttccacct ttccctttta
    12601 aaaaaataga aaaagaatgt cctaatattg aaagaatacc acttgcaagg ataaaaggac
    12661 ttgaaaacat aagaatttta cctgtataaa ctggaataaa tccttttaaa atatcaagaa
    12721 ttaaggcagg aattcccagt ttccaaccgc aaactctata aacattagta gcaccaatat
    12781 ttccgcttcc aaatttgcga atatctatcc ctttgaataa tttacataac agatatccaa
    12841 atggaatact tcctgcaaaa taagagaata ctaaataata aaatttattc acattataaa
    12901 ttaataagtt ccttaacttt atctactgca cttgcagcat caggtgcata gccgtcagca
    12961 ccaatttcat ttgcaaattc ctgagttaca ggtgctccac caataattat ttttacatta
    13021 tctcttacac cttccttttt caaaacttca attgtttcct tcatttgaac cattgttgtt
    13081 gttaagagtg cactcattcc aataacctgt actccgcttt ttgctgcttc aacaaatttt
    13141 tctggtggaa catctatacc aaggtcaaga acttcaaagc cagcaccttt ccacatcata
    13201 acaaccaaat tctttcctat atcatgtaaa tcacctttaa ctgttccaat tgcaagtttg
    13261 gcaattggtt ttattcctgc ctgtacaatt aatggttcaa gtaaactcat tcctttattc
    13321 attgctcttg ctgctattaa aacttcaggg acataatatt cattattttt aaacttttct
    13381 ccaaccacca tcattccagc aatcaaaccc tcatttaata ttttttcaac aggcaaactt
    13441 tccttaattg ctttatcaac taattcttct gtttttttca tatcaccttt aattacactt
    13501 tccttaatcc catttaataa atcatttgcc attttttcct cctttttgtt ttaatttgtt
    13561 ttatttaatc atattttttc tttttgtcaa tatagattca caaacatcaa taaaagtatt
    13621 ttacataaaa ttataaaaat tgtaaaataa atctattagg gcggttagct cagtggttag
    13681 agcactaccc ttacaaggta ggggccgcag gttcaagtcc tgcaccgccc atttaagatt
    13741 ttaatattat tcattttaat gaaacttctt taatttataa ctcatctaaa ttaaaaaaaa
    13801 aggaggaata aaaatggcag aaaaaattat aggaattgac cttggaacaa gtaattcatc
    13861 tgcagcaatt atggaaggag ataaacctgt tataatccca agtgcagagg gtactactat
    13921 tgttggtggt aaggcatttc cttcttatgt tgcatttaca aaggatggac aggttcttgt
    13981 tggtgaacca gcaagaagac aggcagcagt taatcctgaa ggaactgttt ttgcagcaaa
    14041 aaggaaaatg ggaacagatt ttaaatatca catatatgga aaaacatata ctcctgaaca
    14101 gatttctgct tttatattac agaaaattaa aaaagatgct gagaattttc ttggttatcc
    14161 tattaaaaaa gcagtgataa ctgttcctgc gtattttaat gataaccaga gacaggcaac
    14221 aaaagacgca ggaactattg ctggtcttga agttgtaagg ttaattaatg agccaaccgc
    14281 tgcttcacta gcatatggac ttgataaact taaagaagaa ttgaagatac ttgtttttga
    14341 ctttggtgca ggaactcttg atgttactat aatggagatg atgggtgggg tttttaaagt
    14401 tctatcaaca agtggagata cacaacttgg tggtacagat atggataatg cattaattga
    14461 atatattgta gaacagttta aaagagaaac aggtatagat ttgaggaatg ataaaatggc
    14521 tatgatgcga ttaaaggaag cagcagaaaa agcaaaaata gaattatctt cggttgttga
    14581 aacagaaata aatttacctt ttatcacagc tgaccagaca ggaccaaaac atttaataat
    14641 gaaaataaat agagcaaaac ttgaagaact catttctcca attatagata gatgcaaagg
    14701 tccagtggat agagcaattg aagatgcgaa acttaaaagg tcagaaataa ataaagtgat
    14761 tcttgtagga ggtcctacaa gaatgccaat agttcagaaa tttattcagg attatatcgg
    14821 aattcagcca gaaagaggag ttgaccctat ggaatgtgtt tctattggag cagcaattca
    14881 gggtgcaatt cttgccggtg agatgaaaga taaggatatt ttacttcttg atgttacacc
    14941 tctttctctt ggagttgaaa cacttggtgg tgtctttact aaaataattg aaagaaatac
    15001 aactattcct gtaaaaagaa gtcagatatt tacaactgcc actgataatc agacatctgt
    15061 tgaagtacat gttcttcaag gtgaaagacc tatggcaaag gataatcttt ctcttggtag
    15121 atttcatctt gatggtattc cacctgctcc gagaggtatt cctcaaattg aggttacttt
    15181 tgatattgat gctaatggaa ttttacatgt ttctgcaaaa gaccttggaa ctggaaaaga
    15241 acaatctata agaataaccg gttcaaagaa attatctcag gaagaaattg aaaaaatggt
    15301 taaagaagca gaaagatttg cagaagaaga taaaaaatta agagaaatag cagaactttc
    15361 aaatcaggca gattctttaa tttatacagt taacaagaca ttgaaagaac atggaaataa
    15421 attgagtgag agtgaaagaa aagagataga ggaaaaaata aacaatttaa gaaaaataat
    15481 tgattcagaa acaaaaacag cagaccagat aagaaaagga atagaagaac ttgagacagc
    15541 atcccataaa cttgctcaga ttttatacca acaagcacag caatcttatg gtagcgcaca
    15601 gagtcagggt cctcagtcag gtcaggaagg aaaacaagaa aatgaaaaag ttgttgatgc
    15661 agaatataaa gtagaggacg ataaaaataa agaacaataa gttttataaa aagctgttta
    15721 gaaatttatc cctctataag ttttagatgt tttagggttg taagagaggt tctttcatat
    15781 tcatcaagtt ttatctgaat ataccttatt gcctcttcta tatttggaat taaaacatat
    15841 tcaagggcat taactcttct tctggttctt tcaatttctt ccgcaaaact tatcaattta
    15901 ttttcaagtc catataaaat aaatagtttt tttaaaatcc tcatcccttc tttaatcata
    15961 aaattttcat agggagagag aaaataatta aaaacaaatt ctccagaaat atatgtttca
    16021 atttcattta caggaatatt aaatatcctt tttctattta aactgtattt gatttcaata
    16081 aatggtattt ttaaaagcga attccatctt ttttcatcaa ttgaacctct taaaattaat
    16141 accttttcac aaaaattaat aaattcattt tcaatcaatt ttctttcttt tttaacaata
    16201 gaaatcagtt ttctgaattc aattaaaagt tgttcttctt tgtctttcag aagtttatgt
    16261 cctctctttg caatagcaat tcttttcttt aatttcaata attccattct tgttggattt
    16321 acctttattt tcattttaat ccttccagaa tttttccatt atgaaaggag ggattctctt
    16381 aatatctatt tttggcaaca ttttaattaa tttccatcca agatttaatg tttcctcaat
    16441 acttctgttt tcattcaatc cctgatttat gaattcctct tcaaattttc ttgcaaaatt
    16501 taaatgggca atatctgatt ctgttaaagc cgcctctcca agaaccattg caagttcttc
    16561 tgcttcctta cctctcgcat atgatgcgaa aagttgactt gcaagatatg gatggtcttc
    16621 ccttgtaagt ttttctcctg tttctttttt aatccctata cctttgtctc tcaatcttga
    16681 taatgaagtt aatatatcca caggtggata aattcctttc aaatgcaatg ctcttgataa
    16741 aattatctgt ccctctgtta tatatcctgt taaatcaggt attggatgtg ttttatcatc
    16801 ctgtggcatt gttaaaattg gaatttgagt tattgaacct ggttttcctt ttattcttcc
    16861 tgctctttca tatattgttg caaggtctgt atataaataa gaaggataac ctcttcttcc
    16921 gggtatttca tctcttgctg ctgatatttc tcttaatgct tctgcataat ttgtcatatc
    16981 agttaaaatt acaagaacat gcataccaag ttcaaaagca agatattcag cagcagttaa
    17041 tgcaa
//
LOCUS       DAOSAT010000032        16465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2420_length_16465_cov_5.420536, whole genome shotgun sequence.
ACCESSION   DAOSAT010000032 DAOSAT010000000
VERSION     DAOSAT010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16465
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2420_length_16465_cov_5.420536"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1444)
                     /locus_tag="PLF90_04020"
     CDS             complement(161..1444)
                     /locus_tag="PLF90_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOK56480.1"
                     /translation="MTEIPLSSYLILLFLIFLSAFFSASETAFISLGKYHYQKLLEID
                     RKKSEKLKFWFENPEKILITTLVGNNFVNILASVFAATIFYQHLNRIIPSVIAGIMTF
                     IILVFGEIIPKSIAKKHFEKIALLTSYPLKIFSFIFYPFVKILLFISNVFLMIFRERI
                     DTIMPVLTEEDVKAMISAGEKEGVIEKEEKEMIHSIFEFGDKMVKEIMTPRVNIVAVE
                     ENTSLEDIVKKISLSGYSRVPVYKDNIDKITGIVYIKDIIARQTEEAWEKLKAKDILR
                     NVYFVPETKKVIELLRELQMLKLQMAIVIDEYGGTAGLVTIEDLIEEIVGEISDEFKK
                     DEKEYIKISEGKYLIKGSMEIEKVEELLKIKVPPEIDIETIAGFVLSYFGRFPKKGEE
                     FEYENYKFVVQDADEKIIKWVKVEKLEKEEGEKYE"
     gene            complement(1441..2004)
                     /locus_tag="PLF90_04025"
     CDS             complement(1441..2004)
                     /locus_tag="PLF90_04025"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOK56481.1"
                     /translation="MNMEIEGYPVYFYKEVTSTMDIAKNLIKENKEGIVVAEIQTEGR
                     GRYGRKWLSPEGGLYFSFVLKKSKITDFLSEIISLSLVETMKNFGIENCKIKFPNDII
                     INEKKICGILIEKIDEFYIAGIGINVKKSKELEENKYICMEDILERKVVIKSVFEVFL
                     RNFRKLKDLSEKNVELVLKNWSENLLK"
     gene            complement(1988..2713)
                     /locus_tag="PLF90_04030"
     CDS             complement(1988..2713)
                     /locus_tag="PLF90_04030"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOK56482.1"
                     /translation="MNLTIDIGNKRIKAGVFKGKRIYRFFSCLNSEFGKISFPEDWKN
                     FKIKKCGISSVFPDLNFLVEKEVKKIFKIEPLFLNYKICGMKLKIKNPEKIGIDRIVN
                     CKGASEIFGWPVIVIDIGTGLTIDVVDKNKFFIGGFILPGPELWINSLKNTALIKEIE
                     EKKCRSIGKDTSSAINLGLLYGISGSIEKIVSKIKKDFETSNVVLTGGWSEKIYRLLS
                     FEKKLRKYLTLEGINIILDEYGN"
     gene            3047..4312
                     /locus_tag="PLF90_04035"
     CDS             3047..4312
                     /locus_tag="PLF90_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOK56483.1"
                     /translation="MRSRILKILIAVFAVALIAPAFSAVENVKVGGDIAVYGILRGDF
                     DAWDTEKIHAFQTAARVYVSVDLTDNVSTMIRVINENVWGNVDWEYGNFEGILLDLAY
                     IKVSDLLTPGLSLTVGRQEIQLGEGLVVGSRFRADMYPDVGHLAAVDLGLRKAFDAIR
                     LDYAASAMPLNITAFMSKIDEEYDDTEDTDLYGLNLGFKLAEVANFDVYYVKEMIRED
                     NMSLDTVGVRAVVGIPAVEGLSMKAEFAKQFGDYDDVVDFSGWALLAGFEYKMATAME
                     PTIKLNYARYSADDEDTDNEDEGWQLVFPSNTGSRIGPILYAYDTFKENEFDYVESGN
                     AQFINLGISFKPAEKVKISIDAYWDRFIQPVAGTKDIGFEVDLGIEWAISEDLTFGIC
                     GGKLFGGDLFENADLADPWQIIASMKVAF"
     gene            4469..5026
                     /gene="pyrE"
                     /locus_tag="PLF90_04040"
     CDS             4469..5026
                     /gene="pyrE"
                     /locus_tag="PLF90_04040"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOK56484.1"
                     /translation="MIQDEELKKRLKKIILEKGVFFKEVVLSSGKKSNFYIDCRVITL
                     SPEGIYLVSKIIFEIIKNEDIDAIGGLTLGADPIVSGVSLISFIEKKPVNAFIVRNIQ
                     KEHGMGKLIEGNIEKGWNVAIVDDVATTGSSIIKAISTVENFGAKVKKVICIVDREEG
                     AKEIIAKKGYKLEPIFTRSELVQIG"
     gene            5019..5873
                     /gene="nadC"
                     /locus_tag="PLF90_04045"
     CDS             5019..5873
                     /gene="nadC"
                     /locus_tag="PLF90_04045"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOK56485.1"
                     /translation="MDEIIRKALEEDIGNGDITTNGIFDENFFISADLVCKSKGLLCG
                     IEVFKRVFLFLSPDFKFNFKYNDGDYISKKTKIGEVIGPVKELLKGERTALNFLQLLS
                     GIATETRKLVEKTEGKFKIYDTRKTHPNLRFLEKYAVKMGGGENHREGLYDMILIKDN
                     HIKALTEKEKIDKISAIKKAIQKAKEYTKGKYKIEIEVENYDEAVCAYSEGVDIIMFD
                     NSDIDDVRKFCEFKKGKKDCEIEVSGNIDLKKIEILKKLNIDRVSIGYITHSPEAVDF
                     SLKIKKAI"
     gene            5947..6714
                     /locus_tag="PLF90_04050"
     CDS             5947..6714
                     /locus_tag="PLF90_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOK56486.1"
                     /translation="MEYFDTHCHLDFEQFDNDRKEVIERAKNQNVEYILNVGTGIESS
                     KKVIEIAEKYDFIFASVGIHPLDISDYDIEDLKEIEKLAKREKVIAVGETGLDYYYSK
                     ENKEKQKDFFRAQIEIAGKYNLPLIIHQRESKNELIEMIEKVKIPEKVVFHCFGGDNE
                     LAEYCKKRGFFISFTGILTFKNAKNVKEVAKIYPVEKIMVETDAPFLAPQIFRGKRNE
                     PFMVKYIVKELADLKGENEGKIADIIFENSLNFFLKI"
     gene            6745..7200
                     /locus_tag="PLF90_04055"
     CDS             6745..7200
                     /locus_tag="PLF90_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOK56487.1"
                     /translation="MIDGVKIKVLKIIPDERGRLMEILRRDDDIFTEFGQVYITTAYP
                     GVVKAWHAHRFQDDNITVIKGMAKIVLCDLREGSSTYKKINEFFVGDYNPVLIHIPKL
                     IWHGFKCISENECIVLNIPTNVYNYEKPDELRLPYNTPEIPYDWERRNY"
     gene            7197..7940
                     /locus_tag="PLF90_04060"
     CDS             7197..7940
                     /locus_tag="PLF90_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOK56488.1"
                     /translation="MKGIVLAGGLGTRLYPLTKVTNKHLLPVYNKPMIYYPIQTLVDA
                     GIKDILIVTGGNSAGDFLKLLGNGREFGLSHLSFVYQEGESGIADALSLAEHFCDGDK
                     MVVILGDNIIEKSIKKEVEIFEKQPKGARILLKEVENPERFGVPEIKDGKIVRIIEKP
                     KIPPSKFAVTGIYMYDSQVFDIIRTLKPSERGELEITDVNNKYLEIGQLTYGILEGWW
                     TDAGTFDSLLRANILVAQKIKEEDGAQKR"
     gene            7937..8929
                     /gene="rfbB"
                     /locus_tag="PLF90_04065"
     CDS             7937..8929
                     /gene="rfbB"
                     /locus_tag="PLF90_04065"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOK56489.1"
                     /translation="MKIVITGGAGFIGSNFIKYILNKYPDYKVLNIDKLTYAGNLENL
                     KGVEGNPNYNFLKKDICDRDIEDFINGYDVIINFAAESHVDRAIYSPEDFLKTDIFGT
                     FNLLEVCRKKKIRFIQISTDEVYGSIEEESFDENSPLNPSNPYSASKGGADLLTLSYF
                     KTYKLPVNIIRSCNNYGPNQYPEKFIPLMITNAIENKKLPIYGDGLYRREWIYVIDNC
                     KAIDTVLHKGKDGEIYNVSSEFSIPNIEIAKKILKILCKEESLIEFVNDRPGHDRRYS
                     IRCEKIKELGWKTETDFETGINLTVKWYIENKNWWHNIKKSSSFISHYKKIYNL"
     gene            complement(8933..10234)
                     /locus_tag="PLF90_04070"
     CDS             complement(8933..10234)
                     /locus_tag="PLF90_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOK56490.1"
                     /translation="MKKVLFLFVLLSFFVFSEIKFDYGFDLRLREEYLGNIFAPVGKD
                     DDNYFRVKASLWGKWNFGENNVIFIKFSGEPRFYLERGGFLSRERYRSDDEFIFENLY
                     FEFKKIFGSNFDLKIGRQDFLGQYGEGFIIQEGTPGDGSRTFYFNALKLTYHFNEKNL
                     IDFIYYNDPKEDFMPIINNNHKSLTNSDEKGVIVYGKFNPFQNINLTLEPYYIYKDQG
                     TYTISYTIPNPTIIPALEFNTFGIRKVYSFDPWKLRGEIAYQWGEYENGTDKRAWAGY
                     AFLTRSFKDVKFSPSIDLGYVYLSGDKSDTLKDEGWDPVFGRWPWISELLLYYYFYDG
                     EIAKFTNYNLWRASLNLIFSKNTNLALSYNYFRANEPPVSSPLLGTGKERGHLPTLIL
                     RHKFTQNISSHFWFEYFVPGDYYSPNRKNCTFVRWEISMRF"
     gene            complement(10253..10663)
                     /locus_tag="PLF90_04075"
     CDS             complement(10253..10663)
                     /locus_tag="PLF90_04075"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56491.1"
                     /translation="MGKQISIFVENKPGKISKITEVLSKGKINLKAMTIADSGSFGII
                     KIMTDDNQKAYEILKENNFLVSLQEVVVVEIPDKVGSFHKIAKILEKDKINIEDAYGF
                     IVQEGVKAVLILKVKDVKKVEDVLKSKGYKVIQF"
     gene            complement(10664..11983)
                     /locus_tag="PLF90_04080"
     CDS             complement(10664..11983)
                     /locus_tag="PLF90_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOK56492.1"
                     /translation="MKQKQVLLSNVEYWDEKIELVEKSKLKDIQLEGLISSFERGIKS
                     KFYREKFKKFKKIKDIEDFQELPFTTKYDLVENDPFDFISTDIKNIVRVHSSSGTTGR
                     SKYIFYTKKDIDTWADIVARCLYMIGVREGDVFQNMMSYGLFTGGLGLHYGAERIGAL
                     IIPAGAGNTERQIELMKQLKTTVIHITPSYLLYIAHFIEENGLSPKRDFNLKIAVVGA
                     EPHSESTRKKLENIFGINIYNCYGLSEMNGPGVAFECPFKDGLHLWEDQYYMEIINPK
                     TGDVLKEGEEGELVLTTLNREGMPLIRYRTGDLTRIIKERCKCGRTHRRIQRIKGRAD
                     DMFIVKGVNIFPSQIETVIMEFPEVDKNYQIIIDRKEGLDILTVQIEIKREFFKGDIN
                     ELRNLQEKIKEELREKILVTPVIELVEPGSLPTTTGKAKRVIDKRRL"
     gene            complement(11949..13445)
                     /locus_tag="PLF90_04085"
     CDS             complement(11949..13445)
                     /locus_tag="PLF90_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOK56493.1"
                     /translation="MGKFFIVIIYLVIMLYIGAIASKKIKNTSDYIVAGKTLGFWLFV
                     LLILGSTTSGMTILGVTGLGFVAGWPSFWEQIFVPLTCAIAITIYGYKIYCVCRDKNF
                     LTLQDYLSYRYESESLVRFISSFAVLVTSLIYLVGQYTAISIVLKWALGIGQIQALII
                     GTIIVLIYVLLGGLYAVSWTTLFQGIIIILGVLLTCPFIIKSAGGLTYINQTLVEIDP
                     NYVKIAFPQVHPPYAPYAFCTPLYIISFFFLLALGLGSGPHIINNVIAVKDKKYFKWS
                     GLIVFVIYLIIMFLVKITGMAVKTLVYEGKVTLTKPDDALLAGLTYVFSDKFMGFFAV
                     IILAAVMSTTDRLLLVIGSTCGWDFYKKLINKNADDRKVTLISRIFIVIFALISFVLA
                     IKPPQLLAFLIWTAIGVMLSTFVPSILFGLYWKRANKYGAICSMISGFVFAFIFGWYY
                     KFKKPLPFHPSFYSFLISVFVMIFVSLITKKTSQEILNETKTGPFIKC"
     gene            complement(13582..14013)
                     /locus_tag="PLF90_04090"
     CDS             complement(13582..14013)
                     /locus_tag="PLF90_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56494.1"
                     /translation="MSIKQLSIFLENRLGRLNEVISLLGKNKINIRALSLADKADYGI
                     LRLIVNDKKKAFKVLKDNNFTVDETDVIAVEVEDKPGGLAKILEYLVEGGISIEYVYA
                     FVEKSNKKAVVVLKTEDIKKTEKILKKNKVKLLKESDVKRI"
     gene            complement(14014..15327)
                     /locus_tag="PLF90_04095"
     CDS             complement(14014..15327)
                     /locus_tag="PLF90_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOK56495.1"
                     /translation="MEYFQKEIEKISEKELKKIQFERLKKVFNLAKEKIPFYRKLYEK
                     AGIKNITSLSDLKNLPFTKKSDLRDNYPFKMFAYPLKDIFEIHASSGTTGKLTVVGYT
                     KNDIEVWSEVMARSLVCAGVKKGDIIQNAYGYGLFTGGLGVHYGALKLGVTVIPISTG
                     GTQRQLMIIQDFKSTVLTCTPSYSLYMAEEAKEMGIDPKKTTIRVGILGAEPWTEGMR
                     KKIEEEWDMIALDIYGLSEIIGPGVAMECEGKDGLHVWADHFLPEVINPETGENVDEV
                     EEGELVLTTLTKEGLPMIRYRTGDIVKITYSECPHCKRTMPRISKIKGRIDDMLIIRG
                     VNVFPSQIETVLTKIPEVSPHYQLVVSREKYLDKLEVRVEVTEKTFSDEVKKLEELQK
                     KIEKTIEGVIGLSISVKLVEPKSIERSIGKAKRVIDLRKELKEEK"
     gene            complement(15420..16229)
                     /gene="queG"
                     /locus_tag="PLF90_04100"
     CDS             complement(15420..16229)
                     /gene="queG"
                     /locus_tag="PLF90_04100"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOK56496.1"
                     /translation="MDEIKKKIIEISKKSNFDLIGFSRCEKVENEFIDFYMTYLKNKY
                     NGELEYLEKNLEKRFNPEKLLLSVKTIISLGVNYYSQIPENCVFPRYLTKIDYHKVIK
                     KMLKVFIENLKKEIGKFEYRFFVDSGPIFEKYWAKKAGNGFIGKNSLIINKDFGSFIF
                     LCEVLINVEIEPDKPVENLCGKCKRCIESCPTGAIIEDGIIDVRKCISYLTIEKKELS
                     EFEKDLIKKGNKIFGCEICQEVCPFNKNLKETDKEELKIPDRLKNIEFSDI"
     gene            16393..16465
                     /locus_tag="PLF90_04105"
     tRNA            16393..16465
                     /locus_tag="PLF90_04105"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16427..16429,aa:Glu,seq:ttc)
BASE COUNT         5853 a         2558 c         2259 g         5795 t
ORIGIN      
        1 tccatgacct ctaatagcca taattctacc agcaatttta aaactaccat cttccttaac
       61 ttctccaata tttgttttaa aaaatcttct tccgtaaact tcaattcctt cttctttaag
      121 ttcttcaagt ttttttattc ttacctctct catttgattt tcactcatat ttttctcctt
      181 cctctttttc taatttctca accttaaccc atttaataat cttttcatct gcgtcctgaa
      241 caacaaactt ataattttca tattcaaact cttctccctt tttaggaaat cttccaaaat
      301 atgataaaac aaaaccagcg attgtttcaa tatcaatttc tggtggaact tttattttta
      361 gtaattcctc aactttttca atttccatac tcccttttat aagatatttt ccctcactta
      421 tttttatata ttccttctca tcttttttaa attcatctga aatctctcca acaatttctt
      481 ctataaggtc ttctatggtt acaagaccag cagttccgcc atactcatct ataacaattg
      541 ccatttgtaa ttttaacatc tgtaattctc ttaacaattc aataactttt tttgtttcag
      601 gaacaaaata aacatttctt aatatgtctt ttgctttaag tttttcccat gcttcttctg
      661 tctgtcttgc tattatatcc tttatataga caattcctgt aattttatct atattatctt
      721 tataaaccgg aactcttgaa tatccagata aactgatttt tttaacaata tcctcaagag
      781 atgtattttc ttcaaccgca actatattaa ccctcggagt cattatttct ttaaccattt
      841 tatctccaaa ttcaaatata ctgtgtatca tctctttttc ttctttttct ataactcctt
      901 ccttttctcc tgctgaaatc attgccttta catcttcttc tgttaaaaca ggcattattg
      961 tatcaattct ctccctgaaa atcatcagaa aaacatttga aataaaaagt aagattttga
     1021 caaaaggata aaaaataaaa gaaaaaatct ttaatggata tgatgttaaa agagcaattt
     1081 tttcaaaatg ttttttagca atacttttag ggattatctc accgaaaaca agaataataa
     1141 aggtcataat ccctgcaatt actgatggta taattctgtt taaatgttga taaaaaattg
     1201 ttgctgcaaa gacagaagca agaatattaa caaaattatt tccaacaagt gtagtgatta
     1261 atatcttttc tggattttca aaccagaact tcaatttttc actttttttc ctgtcaattt
     1321 caagaagttt ttgataatga tatttaccaa gagaaataaa ggcagtttca gatgcagaaa
     1381 aaaatgcaga aagaaaaatt agaaataaaa gtatcaaata agaacttaac ggaatttcag
     1441 tcattttaaa agattttcac tccagttttt tagaaccaat tctacatttt tttctgataa
     1501 atctttcaat tttctaaaat ttctcaaaaa aacttcaaaa acacttttta ttaccacttt
     1561 tctttccaaa atatcttcca tacatatata tttgttttct tcaagttcct tacttttttt
     1621 tacatttatt cctatacctg ctatataaaa ttcatcaatt ttttcaatca gaattccgca
     1681 tatctttttc tcatttatta ttatatcatt tggaaacttt attttgcaat tttctattcc
     1741 aaaatttttc attgtttcaa ccaaacttaa tgaaattatc tctgaaagaa aatcagtaat
     1801 tttgcttttt ttcaaaacaa atgaaaaata taaaccacct tcaggagaca gccacttcct
     1861 tccgtatctt ccccttccct ctgtttgaat ttcagcaaca acaattccct ctttattttc
     1921 ttttatcaga tttttagcaa tatccattgt agaggtaact tctttataga aataaactgg
     1981 ataaccttca atttccatat tcatcaagga ttatatttat tccttcaaga gttaaatatt
     2041 ttctcaattt cttttcaaaa ctcaaaagtc tgtaaatttt ttcactccat ccacctgtaa
     2101 gaacaacatt tgaagtttca aaatcttttt taatctttga aactattttc tcaatacttc
     2161 ctgatattcc ataaagaagt ccaaggttta tggcagaaga tgtatctttc ccaatacttc
     2221 tacatttttt ttcttctatt tcctttatta aagcagtatt ttttaaagaa ttaatccaca
     2281 attcaggacc tggtaatata aaaccaccta taaagaattt atttttatca acaacatcaa
     2341 ttgttaaacc agttccgata tcaataacta taacaggcca accaaaaatt tcagaagcac
     2401 ctttacagtt aacaattcta tcaataccta ttttttcagg attttttatt ttaagtttca
     2461 taccgcagat tttataattc aaaaacaatg gttcaatttt aaaaattttt ttaacctctt
     2521 tttcaactaa aaaattcaaa tcagggaaaa cagatgaaat accacatttt tttattttaa
     2581 aatttttcca atcttctgga aaggatattt ttccaaattc agaattcaaa caggaaaaaa
     2641 atctgtaaat tctttttcct ttaaatacac ctgcttttat tcttttattt ccaatatcaa
     2701 ttgtaaggtt cattttcttt aaataaaatc taaaccaaaa acataaaatt ttcaagtttt
     2761 aatttcctct tgacaaaaat aaaaagatgt gtataaataa aaattgaata tgttaaactt
     2821 gacaaatttg aaaatttgag tataaaataa acttgacaga ttgaaaattt aaaggtataa
     2881 taaacaaaga gtttatgcta cttcttcctg actttaaaaa tgccggaaag gaggtgaaga
     2941 aaaaacaaaa aggcagaaag tcagaaaaag aagaagagca taaacaaagg aaaagagatt
     3001 tgtcccgaaa aggataagtt atagataaca aaaggaggaa gtaaaaatga gaagtagaat
     3061 attaaaaatc ttgatagcgg tttttgcagt tgctttaatt gcacctgcat tttccgctgt
     3121 tgagaatgta aaagttggtg gagatattgc tgtttatgga atattaagag gtgattttga
     3181 cgcatgggat actgaaaaaa ttcatgcttt ccagacagca gcaagagttt atgtttcagt
     3241 agaccttact gataatgtat ctacaatgat tagagtgatt aatgaaaatg tatggggaaa
     3301 tgtagattgg gaatacggaa actttgaagg aattcttctt gaccttgctt acattaaagt
     3361 ttcagacctt cttacaccag gactttctct tacagttgga agacaggaaa ttcaactggg
     3421 agaaggactt gttgtaggaa gtcgttttag agcagatatg tatccagatg ttggacacct
     3481 tgcagcagtt gaccttggac tcagaaaagc atttgatgct atcagattgg actatgctgc
     3541 aagtgctatg cctttaaaca taactgcttt catgagcaaa atagatgaag aatatgatga
     3601 tacagaggat acagaccttt atggcttgaa tcttggattt aaacttgctg aggttgctaa
     3661 ttttgatgtt tactatgtaa aagaaatgat tagagaagac aatatgtcac ttgatacagt
     3721 tggtgtaaga gcagttgtag gaattccagc ggttgaagga ctttcaatga aagcagaatt
     3781 tgcaaaacag tttggagatt atgatgatgt agtagatttc agtggttggg ctttacttgc
     3841 tggatttgaa tacaaaatgg caactgccat ggaaccaaca attaagttaa actatgcaag
     3901 atattcagca gacgatgaag atacagacaa tgaagatgaa ggatggcaac ttgtatttcc
     3961 atcaaacact ggttcaagaa ttggtccaat cctttatgct tatgatactt tcaaagaaaa
     4021 tgaatttgac tatgtagaaa gcggaaatgc tcagttcatc aatttaggaa tttcttttaa
     4081 acctgcagaa aaagtaaaaa tttcaattga tgcatactgg gataggttta tacaaccagt
     4141 tgcaggaaca aaagatatcg gatttgaagt tgaccttgga attgaatggg caatttcaga
     4201 agaccttaca tttggtatct gcggtggaaa actttttggt ggagatttat ttgaaaatgc
     4261 agaccttgca gacccatggc agattattgc ttctatgaaa gttgctttct aattgataca
     4321 gaaatcaaac cccggctatc tttttggtag ccggggtttt ttattttatc cttctttaat
     4381 tttgtttttt tatccctcac attttttagt aaatagacct ttattaaaaa atttcatatg
     4441 gtaaaataaa taagaaaaag ggataaatat gatacaggat gaagaactta aaaaaagatt
     4501 aaaaaagatt attctggaaa aaggggtttt ttttaaagaa gttgttcttt caagtggaaa
     4561 aaagagcaat ttttatattg actgtagagt aataacccta tctcctgaag gaatttatct
     4621 tgtaagtaaa attatttttg aaataataaa aaatgaagat atagatgcaa taggaggact
     4681 tactcttgga gcagacccta tagtaagtgg agtttcttta atcagtttta ttgaaaaaaa
     4741 accagtaaat gcctttatag taagaaatat tcagaaagag catggaatgg gaaaacttat
     4801 tgaagggaat attgaaaaag gatggaatgt tgcaattgtt gatgatgtcg caacaacagg
     4861 ttcttcaatt ataaaagcaa ttagtacagt tgaaaatttt ggagcaaagg taaagaaggt
     4921 tatatgcata gttgacagag aagaaggagc aaaagaaata attgcaaaaa aaggatacaa
     4981 acttgaacct atttttacga ggtctgaact cgtacaaatt ggatgaaata ataagaaagg
     5041 cactggaaga agatatagga aatggagata taactacgaa tgggattttt gatgaaaatt
     5101 tttttatctc tgctgattta gtatgtaaaa gtaaaggatt attatgtggc attgaggtct
     5161 tcaagagagt ttttttattc ctttctcctg atttcaaatt caattttaaa tataacgatg
     5221 gagattatat tagtaaaaaa acaaaaattg gtgaagttat tggacctgtg aaggaattgt
     5281 taaaaggaga aagaactgca ttaaatttct tacagttatt atcaggaatt gcaacagaaa
     5341 caagaaaact tgttgaaaag acagaaggaa aattcaaaat atatgataca agaaaaacac
     5401 atccgaattt aagatttctt gaaaaatatg ctgtaaaaat gggaggtgga gaaaatcata
     5461 gagaaggtct ttatgatatg attttaataa aggacaatca tataaaagca ttaacggaaa
     5521 aagaaaaaat tgataaaatt tcagcaataa aaaaagcaat tcagaaagca aaagagtata
     5581 caaaagggaa atataagatt gaaattgagg ttgaaaatta tgatgaggcg gtatgtgcat
     5641 attcagaagg agttgacatt attatgtttg acaattctga tatagatgat gtaagaaaat
     5701 tctgtgagtt taaaaaaggg aaaaaagatt gtgaaattga agttagtgga aatattgatt
     5761 taaaaaagat agaaatattg aaaaaattaa atattgatag ggtctctatt gggtatatca
     5821 cacattctcc agaagcagtt gatttttcat taaaaattaa aaaggcgata taaaaatttt
     5881 caaaaatttt tcttaaagaa agaataatat gctttctaat atggaaagta cagaagaaat
     5941 aaaaaaatgg aatactttga tactcactgc catcttgact ttgaacagtt tgataatgat
     6001 agaaaagagg ttattgaaag agcgaaaaat cagaatgttg aatatatttt aaatgttgga
     6061 acaggtattg aaagcagtaa aaaagttata gagattgctg aaaaatatga ttttattttt
     6121 gcatctgttg gaattcatcc tcttgatatt tcagattatg acattgagga tttaaaagaa
     6181 attgaaaaac ttgcaaaaag agaaaaagta attgcagttg gagagacagg tcttgactat
     6241 tattactcaa aagaaaataa agaaaaacag aaagattttt ttagagcaca gattgaaatt
     6301 gcaggcaaat ataaccttcc tttaataatt catcagagag aaagtaaaaa tgaacttata
     6361 gaaatgattg aaaaagtaaa aattcctgaa aaagtcgttt ttcactgttt tggaggagat
     6421 aatgaacttg ctgaatactg taaaaaaaga ggatttttta tttctttcac aggaattcta
     6481 acatttaaaa atgctaaaaa tgtgaaagaa gtagcgaaaa tttatcctgt tgaaaaaatt
     6541 atggttgaaa cagatgctcc atttcttgca cctcaaattt tcagaggaaa aaggaatgaa
     6601 ccatttatgg taaagtatat tgttaaagaa ctggcagatt taaaaggaga aaatgaggga
     6661 aaaatagcag atataatttt tgaaaattct ttgaattttt tcttaaaaat ataaaaaagt
     6721 ttatggtaaa atataaaaat ggaattgata gatggtgtta aaataaaggt tttaaaaatt
     6781 attcctgatg aaaggggaag attgatggag attttaagga gggatgatga tatttttact
     6841 gaatttggac aggtttatat aacaactgca tatccagggg ttgtaaaggc atggcatgca
     6901 catagatttc aggatgataa tataactgtt ataaaaggga tggcaaaaat tgttctatgt
     6961 gatttgagag aaggaagttc aacatataaa aaaataaatg aattttttgt tggagattat
     7021 aaccctgttt taattcatat tccaaaactt atttggcatg gttttaagtg tataagtgaa
     7081 aatgagtgta ttgttttaaa tatacctaca aatgtttata attatgaaaa accagatgaa
     7141 ttaagattac catataatac tcctgaaata ccttatgact gggaaaggag gaattattga
     7201 aaggaattgt tcttgctgga ggacttggaa cccgtttata ccctttaaca aaagtaacaa
     7261 ataaacatct tcttcctgtt tacaataagc cgatgatata ttatccaatt cagacacttg
     7321 ttgatgctgg aataaaagat atacttattg ttacaggagg aaactctgct ggcgattttt
     7381 tgaaattact tggaaatgga agagaatttg gtctttcaca tttaagtttt gtatatcagg
     7441 aaggagaaag cggaattgca gatgctcttt cacttgcaga acatttctgt gatggagata
     7501 aaatggttgt tattcttgga gataatataa tagagaaaag tataaaaaag gaagttgaga
     7561 tatttgaaaa acaaccaaaa ggagcaagaa ttttattaaa ggaagtagaa aatcctgaaa
     7621 gatttggagt acctgaaata aaggatggaa aaatagtaag aataattgaa aaaccaaaaa
     7681 taccaccatc aaaatttgct gttacgggta tatatatgta tgacagtcag gtttttgata
     7741 taataagaac attaaagcca tctgaaaggg gagaacttga aataacagat gtgaataata
     7801 aatatcttga aataggacaa ctaacttatg gaattcttga aggatggtgg acagatgcag
     7861 gaacttttga ttcactttta agagcaaata tacttgtcgc acaaaaaatt aaggaggagg
     7921 acggggctca gaaaagatga aaatagtaat aactggtggt gctggtttta ttggttcaaa
     7981 ttttataaag tatatactta ataaatatcc tgattataaa gttttaaata tagataaact
     8041 tacttatgca ggaaatcttg aaaatcttaa aggggtggaa ggaaatccaa attacaattt
     8101 tttaaaaaag gatatatgtg atagagatat tgaagatttt ataaatggat atgatgttat
     8161 tataaatttt gcagcagaaa gtcatgtcga tagagcaatt tattcaccag aagatttttt
     8221 aaaaacagat atttttggaa cttttaattt acttgaagtt tgcagaaaga aaaaaataag
     8281 gtttattcag ataagcacag atgaagttta tggaagtatt gaggaagaaa gttttgatga
     8341 aaattctcct ttaaatcctt caaatccata ttcagcaagt aaaggtggtg ctgatttact
     8401 tactctatcc tatttcaaaa catataaatt acctgtaaat ataataagaa gttgcaataa
     8461 ttatggacca aatcagtatc cagaaaaatt cataccatta atgataacaa atgcaattga
     8521 aaataaaaaa cttcctattt atggagatgg actttataga agagaatgga tttatgtaat
     8581 tgataactgc aaagcaattg atactgtcct tcataaaggg aaagatggag aaatttataa
     8641 tgtcagttca gagttcagta ttccaaatat agaaatcgca aaaaaaatac ttaaaattct
     8701 ctgtaaagaa gaaagtttaa ttgaatttgt taatgataga ccgggtcatg atagaagata
     8761 cagtataagg tgtgagaaaa taaaagaact tggatggaaa acagaaactg attttgaaac
     8821 aggaataaat ttaacagtca aatggtatat tgaaaataaa aactggtggc acaatattaa
     8881 aaaatcaagt tcttttattt cacattataa aaaaatctat aatttataaa acttaaaatc
     8941 tcattgaaat ttcccatcta acaaatgtac aatttttcct attaggtgaa taataatctc
     9001 cgggaacaaa atactcaaac cagaaatgac ttgaaatatt ctgtgtaaat ttatgcctta
     9061 aaattaaagt tggaagatgg cctctttctt ttccagttcc aagcaaagga gaacttaccg
     9121 gtggttcatt tgctctgaaa taattataag aaagtgctaa atttgtattt tttgaaaaaa
     9181 ttagatttaa agatgctctc cataaattat agtttgtgaa ttttgctatt tctccatcat
     9241 agaaataata atatagtaat aattcactta tccaaggcca tcttccaaaa acagggtccc
     9301 atccttcatc tttgagtgta tcacttttat ctcctgataa ataaacatat cctaaatcaa
     9361 ttgaaggtga aaattttaca tctttgaaag accttgttaa aaaagcatat ccagcccatg
     9421 ctcttttatc agtcccattt tcatattctc cccactgata tgcaatttct cctcttaatt
     9481 tccatgggtc aaaactataa acttttctaa ttccaaatgt attaaattca agtgctggta
     9541 ttatagtagg atttggaata gtataagaaa tagtgtatgt cccttggtcc ttatagatgt
     9601 aatatggttc aagtgtaaga tttatattct gaaaaggatt aaatttacca taaactatta
     9661 cgcctttttc atcagaattg gtaagagatt tatgattatt atttattata ggcataaaat
     9721 cttctttcgg gtcattatag taaataaaat caattaaatt tttttcatta aaatgatatg
     9781 taagttttaa tgcattgaaa taaaaagtcc ttgaaccatc ccctggtgtt ccttcctgaa
     9841 ttataaatcc ctctccatac tgtcctaaaa agtcctgtct tcctattttc aaatcaaaat
     9901 tagaaccaaa aattttttta aattcaaaat aaagattttc aaaaatgaat tcatcatctg
     9961 acctgtatct ctctcttgaa agaaaaccac ctctttcaag gtaaaatctc ggttctcctg
    10021 aaaattttat aaagattaca ttattttctc caaaattcca ttttccccat aaagatgctt
    10081 taactctgaa ataattgtca tcatctttac caaccggagc aaaaatatta ccaagatact
    10141 cttctctcaa tcttaaatca aaaccataat caaacttaat ttcactgaaa acaaaaaaac
    10201 tcaataacac aaataaaaat aaaacttttt tcataaaaac ctcctttctt ttttaaaact
    10261 gaataacttt ataaccttta ctctttaaaa catcctcaac tttttttaca tccttaacct
    10321 tcagaatcaa aactgcctta actccttcct gtacaataaa tccgtaagca tcctctatat
    10381 ttattttatc tttttctaga atttttgcaa ttttatgaaa agaaccaact ttatcaggga
    10441 tttcaacaac cacaacttcc tgtaaagaaa caagaaaatt attctcctta agtatttcat
    10501 atgctttctg attatcatct gtcattattt ttattattcc gaaactacca ctatctgcta
    10561 ttgtcattgc ttttaaattt attttgcctt tacttaggac ttctgttatt ttacttattt
    10621 tccctggttt attttcaaca aaaattgata tctgttttcc catttataat ctccttttat
    10681 caataactct ttttgccttt cctgttgttg tcggtaaact ccctggttca acaagttcaa
    10741 taacaggagt cacaagtatc ttttccctta attcttcctt tattttttcc tgcaagtttc
    10801 tcaattcatt tatatctcct ttaaaaaatt ctctttttat ctcaatctga acagttaaaa
    10861 tatcaagccc ttcttttcta tcaattataa tctggtaatt tttatcaact tctggaaatt
    10921 ccataataac tgtctcaatc tgtgatggaa aaatattaac tccctttaca ataaacatat
    10981 catctgctct tcctttaatt ctctgtattc ttctgtgagt tcttccacac ttacatcttt
    11041 cttttataat tcttgttaaa tctcctgtcc tgtatcttat caaaggcatc ccttctctat
    11101 ttaaagtcgt taatacaagt tctccctctt ctccttcttt taaaacatct cctgtttttg
    11161 gatttataat ttccatataa tactggtctt cccataaatg taaaccatcc ttaaaaggac
    11221 attcaaaagc aactccagga ccattcattt cagaaagtcc ataacaatta taaatattaa
    11281 taccaaaaat gttttcaagt ttctttcttg tactttctga atgcggttct gctccaacaa
    11341 ctgcaatttt taaattaaaa tctctttttg gagataaacc attttcctca ataaaatgag
    11401 caatatataa taaataactc ggtgtaatgt gaataactgt tgttttaagt tgtttcatca
    11461 attctatctg tctttctgta tttcctgctc ctgccggtat tataagtgct ccaatccttt
    11521 ctgctccata atgaagtcca agtcctcctg taaaaagtcc ataactcatc atattctgaa
    11581 aaacatctcc ttctctcact cctatcatat ataaacatct tgcaactata tcagcccatg
    11641 tatctatatc ctttttcgtg taaaaaatat atttagacct tcctgtagta ccagaagaac
    11701 tgtgaaccct tacaatattt ttaatatcag ttgaaataaa atcaaagggg tcattttcta
    11761 caaggtcata tttagtagta aagggtaatt cttgaaaatc ctcaatatct tttatttttt
    11821 tgaatttttt gaatttttct ctgtaaaact tactttttat tcctctttca aatgaagaaa
    11881 ttaaaccttc aagttgaata tcttttaatt tacttttttc aaccagttct attttttcgt
    11941 cccagtattc aacatttgat aaaaggacct gtttttgttt cattcaaaat ctcctgagat
    12001 gtttttttgg taattaaact cacaaaaatc ataacaaaaa cagaaatcaa aaatgaatag
    12061 aaactgggat gaaaaggaag tggtttttta aatttataat accagccaaa aataaaggca
    12121 aaaacaaaac cacttatcat tgaacatatt gcaccatact tatttgctct tttccagtaa
    12181 agtccgaaaa gaattgaagg aacaaaagtt gaaagcataa ctcctattgc tgtccagata
    12241 agaaaagcaa gaagttgagg tggttttatc gcaagaacaa aactgattaa agcaaaaatt
    12301 acaataaata ttctgcttat aagtgttact tttctatcat ctgcattttt atttataagt
    12361 tttttataaa aatcccagcc acatgtactt cctataacaa gtaaaagtcg gtctgttgtt
    12421 gacataactg cggcaagaat aattacggca aaaaaaccca taaatttatc gctaaaaaca
    12481 taagtcagtc cagcaagcaa agcatcatct ggttttgtaa gtgtaacttt tccttcatat
    12541 acaagtgttt ttacagccat acctgtaatt ttaacaagaa acataattat aagataaata
    12601 acaaaaacta taagacctga ccatttaaaa tattttttat cttttacagc aattacattg
    12661 tttattatat gaggtcctga accaagtcca agagcaagta agaagaagaa agagattata
    12721 taaagtggag tacagaaggc gtaaggagca taaggaggat gaacctgcgg aaaagcaatt
    12781 tttacataat tcgggtcaat ttctactaat gtctggttta tatatgtaag tccgccagca
    12841 cttttaatta taaatggaca ggtaagtaaa acaccaagaa ttattattat tccctgaaaa
    12901 agggttgtcc aggaaactgc ataaagacca ccgagcagaa catatattaa aactattatt
    12961 gtacctataa taagagcctg aatctgaccg ataccaagag cccattttaa aacaattgag
    13021 atggcagtat actgaccaac aagataaatt aaagaagtaa caagaacagc aaaagaagaa
    13081 ataaatctga caagagattc actttcatat ctataagaaa ggtaatcctg taaggtcaaa
    13141 aagtttttat ccctgcatac acaatatatt ttatatccat aaattgttat tgcaattgca
    13201 caggttaaag gaacaaaaat ttgctcccag aaacttggcc atccagcaac aaaaccaaga
    13261 cctgtaactc caagaattgt cataccactt gttgtagaac caagaattaa taaaacaaat
    13321 aaccagaaac caagtgtctt tccagcaaca atataatcgg atgtattctt aattttttta
    13381 cttgcaattg ctcctatata caacattatt acaagataaa taataactat aaaaaattta
    13441 cccatttttt tctcctttta cttgaatttt aaagcccaga ttagaaggaa aaggattatg
    13501 agtagaggac aaaaaataaa aaaaactaaa aatgtcaaaa aaggaagtcc aaacatattc
    13561 ctcctttttt aataacttct ctcaaattct ttttacatca ctttctttaa gcaatttaac
    13621 tttatttttt ttcagtattt tttctgtttt tttaatatct tctgttttaa gaaccacaac
    13681 cgctttttta tttgattttt caacaaatgc atatacatac tcaatactta ttcctccttc
    13741 tacaagatat tcaagaattt ttgccagtcc tcctggttta tcctcaactt caactgcaat
    13801 aacatctgtc tcatccactg taaaattatt atcctttaaa actttaaatg ctttcttttt
    13861 atcattaaca atcaacctta aaattccata gtctgcttta tctgcaaggg ataatgccct
    13921 gatatttatc ttatttttcc caagaaggga tattacttca ttcaatctcc caagacggtt
    13981 ttctaaaaat atggaaagtt gttttattga catttatttt tcctccttta actcctttct
    14041 caaatcaata actctttttg cctttccgat acttctttca atactctttg gttcaacaag
    14101 tttaaccgaa atagataaac caataactcc ttcaattgtc ttttcaatct ttttttgtaa
    14161 ttcttcaagt tttttaactt cgtcagaaaa tgttttttct gtaacttcaa ctcttacctc
    14221 aagtttatca agatattttt ctcttgatac cacaagttga taatgaggag atacttcagg
    14281 tatttttgtc agaactgtct caatctggga tgggaaaaca ttaactcccc ttataataag
    14341 catatcatct atccttcctt taattttact tattcttggc attgttcttt tacagtgagg
    14401 acattctgaa taggttatct ttactatatc tcctgttcta tatcttatca ttggaagacc
    14461 ttctttagtt aaagtggtta gtacaagttc tccttcttct acttcatcta cattttctcc
    14521 tgtttcagga ttaataacct ccggtaaaaa atggtcagcc catacatgaa gtccatcttt
    14581 tccttcacat tccattgcta caccaggacc aataatttca gaaagtccat atatatcaag
    14641 agcaatcata tcccattctt cttctatttt ttttctcatt ccttcagtcc atggttctgc
    14701 tccaagaatc cctactctaa tagttgtttt cttcgggtct attcccattt cttttgcttc
    14761 ctctgccata taaagagaat aggaaggtgt gcatgtcaaa actgtacttt tgaaatcctg
    14821 aataatcatt aactgcctct gtgttccacc agttgatata ggtattactg ttactccaag
    14881 ttttaaagca ccataatgaa caccaagacc accagtaaaa agtccatatc cataagcatt
    14941 ctgaataata tcaccttttt ttacacctgc acataccaga cttcttgcca taacttcact
    15001 ccatacctca atatcatttt ttgtataacc aacaacagta agttttcctg ttgttccgga
    15061 agaagcatga atttcaaaaa tatctttaag tggatatgca aacattttaa acggataatt
    15121 gtccctcagg tcactttttt ttgtaaaagg aaggtttttt aaatcagaaa gtgatgttat
    15181 atttttaatt cctgcctttt catataattt tctatagaaa ggaatttttt cttttgctaa
    15241 attaaaaact tttttcaatc gttcaaattg tatttttttt aattctttct ctgatatctt
    15301 ttcaatttct ttttgaaaat attccatact ttttaaaatt tatcatataa aatttttttg
    15361 tcaaattttt taattctctt ttcaaggaca gttcctttaa attctaaaaa attaatattt
    15421 taaatatcgg agaattcaat atttttcaat ctatcaggaa tcttaagttc ttctttatcc
    15481 gtttctttta aattcttatt aaatggacat acttcctgac atatttcaca tccaaaaatc
    15541 ttgtttccct ttttaatcaa gtccttttca aattctgata attctttttt ttcaattgtc
    15601 aaatacgata tacattttct cacatcaatg attccatctt caataattgc acctgtagga
    15661 caactttcaa tacatctttt acattttccg cagagatttt ctactggttt atcaggttca
    15721 atttctacat ttattaaaac ttcacacaag aatataaaag aaccaaaatc cttattaata
    15781 attagagaat ttttaccaat aaaaccattc cccgcctttt tagcccaata tttttcaaaa
    15841 ataggtccag aatcaacaaa aaatctgtat tcaaattttc ctatttcttt ttttaaattt
    15901 tcaataaaaa ctttcagcat tttttttatt accttatgat aatcaatttt tgtcagatac
    15961 cttggaaata cacagttttc aggaatttgg gaataataat taacaccgag agaaataatt
    16021 gtttttacag atagaagtaa tttttcagga ttaaaccttt tttcaagatt tttttcaaga
    16081 tattccaact ctccattata cttatttttc agatatgtca tataaaaatc aataaattca
    16141 ttctcaactt tctcacatct tgaaaatcca attaaatcaa aattactctt ttttgaaatt
    16201 tctattatct tttttttaat ctcatccatt ttcttctttc tataaatttt aaaacttttc
    16261 aagatttttt taaattttac tttttttcaa cttttttagt ttcgctcaaa ataaaatttt
    16321 tgacaatagc gttttaataa ggtataatat tctattatta gaaatttgtt ttaagaataa
    16381 tttgaatgta aagcccccat cgactagtgg tctaggtcac cactctttca aggtggtagc
    16441 gccggttcga gtccggctgg gggcg
//
LOCUS       DAOSAT010000033        16419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2431_length_16419_cov_4.597348, whole genome shotgun sequence.
ACCESSION   DAOSAT010000033 DAOSAT010000000
VERSION     DAOSAT010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16419
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2431_length_16419_cov_4.597348"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /gene="hflX"
                     /locus_tag="PLF90_04110"
     CDS             <1..1127
                     /gene="hflX"
                     /locus_tag="PLF90_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOK56497.1"
                     /translation="EKVKKLIENLPETFVGKRGILVIIKDKSYYEIEEETEELTALSE
                     TLGIKIIEKFYIKLQQINPATYIGKGKLEEIKNFIQKNNISILIFEKDLTPVQQRNIE
                     ETLGIKVIDRTELILYIFGEHAHSKEGKIQVELAQLNYLLPRLTGQGISLSRLGGGLG
                     TRGPGEMKLEVYRRRIKDRIHILKEKIKDIEKHREVLRKSREMFPTASLLGYTNVGKS
                     SLLNSLSSSNLYVADKLFATLDPATRGVYLGDNKICLISDTVGLLKNIPHHLIEAFKS
                     TLEEVKYSDLIICVYDISKPNLDKQKDTITEVLKILAIENKPKIDVFNKIDLLPNEEI
                     EIIKSRYPDFIFVSAKTKEGIEELKKTIKEILYGSKVIST"
     gene            1102..2088
                     /locus_tag="PLF90_04115"
     CDS             1102..2088
                     /locus_tag="PLF90_04115"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOK56498.1"
                     /translation="MEVKLSPLDKKKIEEIIKVMKDKNSFLITSHINIDGDGIGSEIA
                     LYICLKKLNKHVEIINQDSIPAIFKFLPYSKHIKIFSKIKKIDNFEVGIILDSGNLER
                     IGNVKEIIKKIPFIINIDHHITNSEFGNINWINPVFSSTGEMTYFLCEELKHIDKKIA
                     TCLYTSIIYDTCGFLHHISKYTMDIAKNLIDTKINPEKIAQKIFFEKSIKSVILFKLA
                     LETLKFDKRKKICFMRVPAEFFKKAKAKEEHTEGFVDFLISIKGIEVGVLFKEKENGI
                     KVSMRSKGKVDVENIAKKFGGGGHREAAGCFIENKKMEEVESLIKKELRWME"
     gene            2076..2786
                     /gene="truB"
                     /locus_tag="PLF90_04120"
     CDS             2076..2786
                     /gene="truB"
                     /locus_tag="PLF90_04120"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOK56499.1"
                     /translation="MDGIINVYKPGGITSYDVIRIIKKEFNFKDKIGHGGTLDPIGEG
                     VLILLFGRMTKLSNKFLNFDKEYIAEVLLGIKTDTDDIEGKIIEEKQIKNLNEERIIE
                     VIKSFEGETEQIPPFISAIKYKGKPLYKYYRKGIKIQPPPRKVIIKKIEILKIALPYI
                     EFKVLCSKGTYVRSLCRDIGEKLGCGGTQNKLIRTKVGPFKIEDSVRIENLKKYGIEK
                     YLIYLDELLKILKVESVI"
     gene            2783..4114
                     /gene="glmM"
                     /locus_tag="PLF90_04125"
     CDS             2783..4114
                     /gene="glmM"
                     /locus_tag="PLF90_04125"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOK56500.1"
                     /translation="MIEKLKISVAGIRGIYPSELTPEIAYKFGLAYGFYLKEKNIYIG
                     TDTRISSEVLKYALISSLLAKGKNVIDLGIMPTPIIEFAVEKNKRAGGVIVTASHNPF
                     QYNGIKFLSTKGTFLNERENRKLLEIYKKTEISLSKKPGRIKTETQIEDIYFQEIYNN
                     VDIEKIKEKKLKIIVDVCQGVGAIFTKKFLENMNCTVEIINEKPYGIFVHDPEPKKET
                     LKQLSKIVKEKNANIGFAQDPDGDRLAIVDEKGNIIGEEMVLAICVKNILEKIKSPVV
                     VNLSTSMVIDEIAEKFGVKVYRTKIGEVNVVEKMKKIKSLVGGEGNGGVIYSSVHYGR
                     DSFVGMALILDYISRKEKKISEVVSEFPEYFMVKEKYKIEPHKIKEILKKIKNKYSKE
                     KIDYLDGVKIVRKDGWIHIRPSGTEPILRLIIESKDRKILNEYLEEFRAIL"
     gene            4309..4380
                     /locus_tag="PLF90_04130"
     tRNA            4309..4380
                     /locus_tag="PLF90_04130"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4341..4343,aa:Thr,seq:cgt)
     gene            4442..5590
                     /gene="aroC"
                     /locus_tag="PLF90_04135"
     CDS             4442..5590
                     /gene="aroC"
                     /locus_tag="PLF90_04135"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOK56501.1"
                     /translation="MLRFLTAGESHGKCLIGILEGMVSNLSIDVEDINKELERRQKGY
                     GRGERMKIEKDKVEILSGIRNGKTTGAPISLLIENKDWENWKEFMDFKKKKEGKERYI
                     PRPGHADLCGFLKYNYEDLRNVIERASARETAMRVAIGAICKKLLSEFNVRFYSRVKK
                     IGKIEDEGSIEEIKKSYEKIENSPVRCLSREKEMMEEIDKAREKGDTIGGIFEIIVEG
                     ICPGLGSYVHWEKRIDAKISYVLMSIPGVKGIEIGLGFGGSKLFGSEFHDEIFYKNKV
                     YRKTNNSGGIEGGITNGNDLWIKCFFKPIPTVKRGLLSFDIRDFKEKKYEYERSDICA
                     VVPASIIGENLLAFILASEYIEKFAGDSLNDLKENYRIYIERIKKYWF"
     gene            5605..6135
                     /locus_tag="PLF90_04140"
     CDS             5605..6135
                     /locus_tag="PLF90_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOK56502.1"
                     /translation="MEKNIVLIGFMGTGKTEVGILLAKRLNMNFVDTDKLIEEREKDR
                     IVRIFEVKGEEYFREIEEKIIEEVSNYKNCVIATGGGAVIREKNYNNLKKNGILICLK
                     ATPEEIYKRTISKKDRPLLMKSRNVIETIKELLEMRKPYYEKADYILDTTKKPVEIIV
                     EGIIEIIKKEDEKNKS"
     gene            6116..7186
                     /gene="aroB"
                     /locus_tag="PLF90_04145"
     CDS             6116..7186
                     /gene="aroB"
                     /locus_tag="PLF90_04145"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOK56503.1"
                     /translation="MKKIKVKLGESTHYIYIGESIEKTGEKIRDGEFGDKFVILSNQR
                     VFNIYGEKVRKSIEKENRTVEKILIKEGERQKNINTVLKIINKLSDLQISRNDTIVNL
                     GGGVISDIGGFVSSIYKRGIKYITIPTTLLAQVDASIGGKTGINLPFGKNLIGTFYQP
                     SFILIDFKTLSTLPYKEIKQGIAEIIKYGVIKNEKIFEKLEQIKKEEIENNFAFLIME
                     SIKIKVDIVEKDEKETKGLREILNFGHTLGHAIEISDIKKFSHGDSVSLGMIGESYIA
                     YKKGLSDKETYLRIKDLAKKFKLSNSFSKINFEKILEFMKHDKKVRKNKIRFTLPEKI
                     GKVSLGVIIEENEILKILKELL"
     gene            7197..8267
                     /gene="pheA"
                     /locus_tag="PLF90_04150"
     CDS             7197..8267
                     /gene="pheA"
                     /locus_tag="PLF90_04150"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOK56504.1"
                     /translation="MKELEKIRKQIDEIDEKLIELMNLRAELVKKVFEIKNKENKPLF
                     DPSREKKILERLIKKNKGILKPEDIEVIMETIFKVYRGMFKEIVVAYLGPEGTFTHQA
                     ALNKFGKKARYISAKTIDEVFKEVEKERADYGVVPIENSIEGVVTHTLDVFIESDLKI
                     TSEILLEIHHFLLSKENSIDSIKKLYSHPQALAQCRNWISQNLPDAEIYETESTTSAV
                     KKILNEKKSAAIASEIAASMYNLNIIAERIEDFRENVTRFLVVGKNYSEKTGNDKTSI
                     LFSIKDKIGALHDILVPFKKEGINLTRIESRPSKKKAWEYVFFVDFKGHKDEPNVKKA
                     LEKVKKSTIFLKILGSYPKEGG"
     gene            8301..9323
                     /gene="aroF"
                     /locus_tag="PLF90_04155"
     CDS             8301..9323
                     /gene="aroF"
                     /locus_tag="PLF90_04155"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOK56505.1"
                     /translation="MADLIIMRQGATEEEIEKVVEKIEKLGFQAHISKGTERTIIGII
                     GDTRSVSDETFRILPGVSEVISILKEYKLASRDFHPSDTVIDVNGIKIGEGYFVVMAG
                     PCSVENKEQLLTTGKYIKEKGVKILRGAVFKPRTSPYSFLGLGPEGLRIVKEIKEELK
                     IPVITEVLSPEEVEIVSEVSDILQIGTRNMFNYRLLQKVGRINKPVLLKRNFSAKISE
                     FLNCAEYILKEGNNQVILCERGVRTFDSEFTRNILDLSAIPILKKETHLPVIVDPSHG
                     TGRRDLVIPMSKAAIAAGADGLLIEVHPKPEEALSDGFQSLNLEMFGNLMDEITPFIK
                     IMKKKM"
     gene            9325..10617
                     /gene="aroA"
                     /locus_tag="PLF90_04160"
     CDS             9325..10617
                     /gene="aroA"
                     /locus_tag="PLF90_04160"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOK56506.1"
                     /translation="MNTIVRKPEKISGIIELPGDKSITHRSLIFGSISAGKTYILNYS
                     KGKDCLSTLNCLLNLSVKIEEVENQLVINGCEFKEPEDILNCGNSGTTIRLLSGILSA
                     KPLYTVLTGDSSLRKRPMDRIIKPLTMMEAEIYGRGNNKYAPLTIIGKNLKGIKYKME
                     IPSAQVKSCIILATLFANGESIIEESYQTRDHTERMLEYFGGEIEKRGNKIIVKGNQK
                     LKGKEIYIPGDFSSACYFIAGTILVPDSNLIIKNVGLNPTRTGFLNVIERMGGNFEIL
                     RKEEISGEPVGDVKIMYSEKLKGTEILPEEVPNIIDEIPLIAVLGSVAEGKTIVRGAK
                     ELRVKESDRIKSISTELKKLGAKIEEKEDGFILEGVKRLKGTKVNSWNDHRIAMSLVI
                     ASFITEGDIVIENSECVDISFPEFFDKFKYLGVNFEFI"
     gene            10637..11749
                     /locus_tag="PLF90_04165"
     CDS             10637..11749
                     /locus_tag="PLF90_04165"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOK56507.1"
                     /translation="MNGIYFYTPTKIFFERNGIEKYLPLVKNYGNKVLIVSGKNFIYK
                     SGLMDKIKNIFDKENIDYSVFSNVNPEPDIENVEEGVELCKKENCNVILAIGGGSALD
                     AGKCIAVMSKNEGSLKNYFGEVEYKNNPLTVIAVPTTCGTGSEVTRYAVIVDKEEKTK
                     KTVSSEKIMPKIAILDPDLLDYLPSKLIAATGMDAFSHAVESYLATISDYVSKLFSLE
                     SLKLLKENLKRAVDGDKESKEKVFLASTFAGYAINKTGTIIVHGIGYSLTIKYNTHHG
                     TANSLFLPYILEFLKKNNYKKEIEELENIIGTNSELKNFITSIGLPSKLRDIRVKEED
                     IEELVNLSIKGCERAMKKMKFVLTRNDFLEIINSAF"
     gene            complement(11746..13404)
                     /locus_tag="PLF90_04170"
     CDS             complement(11746..13404)
                     /locus_tag="PLF90_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56508.1"
                     /translation="MKKTLPFLVFFFVSIILLCDNFKDRFVWVFGFNIEKNEDLEEVK
                     RILNDAKKNDFNGVILSAGLDSLCKKDERYFRNLKEIRECCEKLNLELIPAVFSIGYG
                     GGVLSHNKYLAEGLPVKDALFVVKNGEAIFISELNVQILNGSFEEFNGNRFKYFNFHD
                     QPGEVSFVEQNIKKSGNTSIRFENFTANPYGHGRIMQEISVKPYRCYRMSIWVKTENL
                     QPQGCFRLLVLGDKKDLAPRSFNLPPTTDWRKLTMIFNSQNFEKVRVYAGVWGGKTGK
                     FYLDDWMIEEIGPINVLRRPGTPITVRNEGSGEIYKEGEDYEPLIDKNFNLWTNFDRP
                     FPKIKITQNSRIKNGQRLRVSWYHPMLIYDSQVTVCMAEPEIYEIFDHEAKLLWETLN
                     YKKVLLNMDEIRMGGTCEACLGKDMAELLGECVKKEVEILRKYNPKAEIYIWSDMFDP
                     NHNAKKDYYLVKGDFTGSWNYIPKDIIIAVWGGRPREKSLEFFYNQGFKVLIACYYDA
                     DNMNDVKLWYEISKKFNNIVGFMYTTWQRKYGFLNVFKDILWNN"
     gene            complement(13404..14216)
                     /locus_tag="PLF90_04175"
     CDS             complement(13404..14216)
                     /locus_tag="PLF90_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK56509.1"
                     /translation="MNKLKLGLIVSSGNLEESFKKVKSFNLSLCQIGFLSEEINRFDP
                     YKIKKKAENYEVEIFSVFMLFKGQIFNNKDGPETMGFIAPKYREERLKLAFDFSDFVK
                     EIGVKNIVCHVGFIPDDEEDPIYKSFLPVMKAVVEKCKENNQIFCFETGQELPSTLKR
                     TILDMRMDNVGINLDPANLILYGKANPIDAVEIFGEYVKSMHGKDGLWPNRDEYLGKE
                     VPIGEGMVNFPVLLRRLKSKGFKGPIIIEREISGEKQIEDIKKAIKFLEPYL"
     gene            complement(14238..15188)
                     /locus_tag="PLF90_04180"
     CDS             complement(14238..15188)
                     /locus_tag="PLF90_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HOK56510.1"
                     /translation="MNLINTIKGSLLENFFPKGWDLKKIDKCCSNPPEKITERQKWWN
                     KDFQPVSCDTFEEFNVKMGHEIAYEICAAKEEGRNLILILPVGPMGMYKWVVYFLKEW
                     NIDCKHVWGFNMDEWSDKEGNTLPPTEPGAFQNAMEDAFYGPLGKLTIPKNQRFFATK
                     NLLPKYPEKINELRKKGAKLVVIFGIGRVFHIAFWEPHFAEEFNSEEEWKKQEYRLGA
                     KLHPLTIEQNAITSFKSRTTLVPCFANTIGPGIFLKADKIIGGADGTLGRGMMWQGMS
                     LWVTLRYGPDIWVPSSFMPTLPGKLFFLKELAGPLIPECN"
     gene            15278..16324
                     /locus_tag="PLF90_04185"
     CDS             15278..16324
                     /locus_tag="PLF90_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK56511.1"
                     /translation="MKENIKFGVIGAGGIATRRTIPGMLEAKNIEVLAVMDIMNAKEI
                     AKKFNIPKYYKKEKDIVKDPDINAVYIASPVYLHLKHIKMSAENGKHILCEKPLCKNL
                     KEGKEAVRICKKNGVFIQEGYMMKFHGAHKKIKELIEKGELGKIVYMRGQLSCWYPPI
                     EGAWRQVPNKGGGGSLIDMATHLYDLLEFFVNDRIKYISAIVNTQVHNYKVEDSSTTL
                     IEFSKGTHATVDCFFCIPDESSKTRLEIYGSKGAILTEGTIGQSIGGKMEGIFGLGES
                     NYDATQSKDIVKEFKEIEFEKINPYTAECEYFADCILRGKKPEINNWENALHILKVTE
                     KAYQSYKKKKIMKV"
BASE COUNT         6531 a         2071 c         2696 g         5121 t
ORIGIN      
        1 gtgaaaaagt gaaaaaactt attgagaatt taccagaaac atttgttggt aaaagaggta
       61 tattagtaat aataaaagat aaatcttatt atgaaataga agaagaaacc gaagaattaa
      121 cagctctatc agaaacactc gggataaaaa taatagaaaa attctatatt aaactccagc
      181 agataaaccc agcaacatat attggaaagg gtaaactgga agaaatcaaa aattttatac
      241 agaaaaacaa tatttcaatt cttatatttg aaaaagacct cactcctgtt cagcagagaa
      301 atatagaaga aactcttggt atcaaagtta tagatagaac agaattaatt ctatatatct
      361 ttggagaaca tgcacattct aaagaaggta aaattcaggt tgaacttgca caactcaatt
      421 atttacttcc aagattaacg ggtcaaggaa tttctctttc tcgtcttgga ggaggacttg
      481 gaacaagagg acctggagaa atgaaacttg aggtatatag aagaagaata aaagatagaa
      541 ttcatatatt aaaggaaaaa ataaaagata ttgaaaaaca cagagaagtt ttgagaaaat
      601 caagagaaat gttcccgact gcttcacttt taggttatac aaatgttggt aaaagcagtt
      661 tattaaactc tttaagttct tccaatcttt atgttgctga taaactcttt gcaacacttg
      721 accctgcgac aagaggtgtt tatctcggag ataataaaat ctgtcttata agtgacacag
      781 ttggtttatt aaaaaatatc ccccatcact taattgaggc atttaaatct acactggaag
      841 aagtaaaata ttcagattta atcatttgtg tttatgatat ttcaaaacca aatttagata
      901 aacagaaaga tacgataaca gaagttttaa aaatacttgc catagaaaat aaaccaaaaa
      961 ttgatgtatt taataaaatt gaccttttac ctaatgaaga aatagaaata ataaaaagta
     1021 gatatcctga ttttattttt gtttcagcaa agacaaagga agggattgag gaattgaaaa
     1081 aaactataaa ggagattctt tatggaagta aagttatctc cacttgataa aaaaaagatt
     1141 gaagaaataa ttaaagtaat gaaggataaa aattcatttc ttataacttc acatatcaat
     1201 attgatggtg atggaattgg aagtgaaatt gctctttata tatgtctaaa aaagttaaat
     1261 aagcatgtgg aaataataaa tcaggattca ataccagcaa tatttaaatt ccttccatat
     1321 tcaaaacata taaaaatatt ttccaaaata aaaaaaatag ataattttga agttggaatt
     1381 atactagaca gtggtaactt agaaagaata gggaatgtca aggaaattat aaagaaaatt
     1441 ccttttatta taaatattga ccaccacata actaattctg aattcggtaa tattaactgg
     1501 attaatcctg ttttttcttc aacaggagaa atgacatatt ttttatgtga agagttgaaa
     1561 catattgata aaaaaattgc tacctgttta tatacttcaa ttatatatga tacatgcggt
     1621 tttctacacc acataagtaa atatacaatg gacattgcta aaaatttaat tgatacaaaa
     1681 attaatcctg aaaaaatcgc tcaaaaaata ttttttgaaa aatcaataaa atcggtcatt
     1741 ctttttaaac tggcacttga aacactgaaa tttgataaaa gaaaaaaaat atgttttatg
     1801 agagttcctg ctgaattttt caaaaaagca aaagcaaaag aagaacatac agaaggattt
     1861 gtggattttt taatttctat aaaaggaatt gaagtcggtg ttttatttaa agaaaaagaa
     1921 aatggtatta aggttagtat gagaagtaaa ggtaaggttg atgttgaaaa tattgcaaaa
     1981 aaatttggtg gaggagggca tagagaagca gcaggctgtt ttattgaaaa taaaaaaatg
     2041 gaagaagttg aatccttaat taaaaaagaa ctgagatgga tggaataata aatgtatata
     2101 aaccaggagg aataacctct tacgatgtaa tcaggataat aaaaaaagaa tttaatttta
     2161 aagataaaat aggacatgga ggaacccttg acccaattgg agaaggggta ttaatcctac
     2221 tttttggaag aatgacaaaa ctttcaaata aatttctgaa ttttgataaa gaatacattg
     2281 cagaagttct tctcggtatt aaaacagata cagatgatat agagggaaaa ataatagaag
     2341 aaaaacagat aaaaaattta aatgaagaaa gaataattga ggtaattaaa agttttgaag
     2401 gagaaactga acaaatacca ccttttattt cagcaattaa atataaagga aaacctttat
     2461 acaaatatta tagaaaagga ataaaaatac aaccacctcc aagaaaagtt atcataaaaa
     2521 aaatagaaat tttaaaaatt gctcttcctt atattgaatt taaagtttta tgttctaaag
     2581 gaacttatgt aaggtcttta tgcagagata tcggcgaaaa acttggttgt ggcggtacac
     2641 agaataaact tataagaaca aaagttggac cctttaaaat agaagatagt gtaagaatag
     2701 agaatttaaa aaaatatgga atagaaaaat atttgattta tctggatgaa ttattgaaaa
     2761 tattgaaagt agagagtgtg atatgattga aaaacttaaa ataagtgttg caggaataag
     2821 aggaatatat ccttcagaat taactccgga aattgcttac aaatttggac ttgcttatgg
     2881 attttatttg aaagaaaaaa atatttatat cgggactgat acaagaattt ccagtgaagt
     2941 tttaaaatat gctttaattt cttcacttct tgcaaaaggg aaaaatgtta ttgaccttgg
     3001 aattatgcca actccaataa tagaatttgc agtggaaaaa aataaaagag cggggggtgt
     3061 aatagttaca gcaagtcata atcctttcca gtataatgga attaagtttt tatcaacaaa
     3121 gggaactttt ctgaatgaaa gagaaaatag aaaattgtta gagatataca aaaaaactga
     3181 aatttcctta tcaaaaaaac cgggtagaat caaaactgaa acacaaattg aagatatata
     3241 cttccaagaa atttataaca atgtagatat agaaaaaata aaggagaaaa aactaaaaat
     3301 aatagttgat gtatgtcagg gagtaggtgc tattttcaca aaaaaatttc ttgaaaatat
     3361 gaactgtaca gtggaaataa taaatgaaaa accgtatggt atttttgttc atgaccctga
     3421 accaaaaaaa gaaacattaa aacaattatc aaaaattgtt aaagaaaaaa atgcaaatat
     3481 tggttttgca caggaccctg atggagacag attggcaatt gttgatgaaa agggaaatat
     3541 aattggagag gagatggttc ttgctatatg tgtaaaaaat attcttgaaa aaataaagag
     3601 tcctgtagta gtcaatcttt caacctcaat ggttatagat gaaattgcag aaaaatttgg
     3661 tgtgaaagtt tacagaacaa aaattggtga ggtaaatgtt gttgaaaaaa tgaaaaaaat
     3721 taaatcatta gtaggtggtg aggggaatgg aggagttatt tattcatctg ttcactatgg
     3781 aagggatagt tttgttggta tggctttaat tcttgattat atatcgagaa aagaaaaaaa
     3841 aatatcggaa gtagtttctg aatttcctga atattttatg gtaaaagaaa aatataaaat
     3901 tgaaccccat aaaattaaag aaatactgaa aaagataaaa aacaagtaca gcaaagaaaa
     3961 gatagattat cttgatggag taaaaatagt aagaaaagat ggctggattc acataagacc
     4021 ttctggaacc gaaccaattt taagattgat tattgaaagc aaagatagga aaattttaaa
     4081 tgagtattta gaagaattca gggctatttt ataaaaaact taaatctcgt cgttcttaaa
     4141 taaaaaaaat tttagaaata gatgtatcat ttaataaaaa tattcaaact tttcaatttg
     4201 caaaacattt ttaagtaata atggtttaat attaatctgc tgataaactt tatcttcttt
     4261 ccttcttaac taaaaatatc tcttaaaaaa tggtaaaatt ataaatgtgc ccgtgtagtt
     4321 cagtggtaga acgacgcatt cgtaatgcgt aagtcgccgg ttcaaatccg gccgcgggct
     4381 ttttaaaaaa taaagtttaa gttataatta tagacaggta tttaaaattt aaggagaaaa
     4441 gatgttaaga tttcttactg ctggtgaatc tcatggaaaa tgtctaattg gaattcttga
     4501 aggtatggtc tcgaatttat ccattgatgt agaagatata aataaagaac ttgaaagaag
     4561 acagaaagga tatggcagag gagaaaggat gaagatagag aaggataaag ttgaaatttt
     4621 aagtggtatt cgtaatggta aaacaacagg tgctccaata tctcttttaa ttgaaaataa
     4681 ggactgggaa aactggaaag aatttatgga ttttaaaaag aagaaagagg gaaaagaaag
     4741 atatataccg agaccaggac atgctgattt atgtgggttt ttaaaatata attatgagga
     4801 tttaagaaat gtgattgaaa gagcaagtgc gagagaaaca gctatgagag tagcaattgg
     4861 agcaatatgt aaaaaacttc tttcagagtt taatgtcaga ttttatagca gggtaaaaaa
     4921 aataggaaaa atagaagatg aaggaagcat agaagaaata aaaaagagtt atgaaaaaat
     4981 agaaaattca cctgttagat gtctttcaag agaaaaagaa atgatggaag aaatagataa
     5041 agcaagagaa aaaggagata ccataggagg gatttttgaa attatagttg agggtatctg
     5101 tcccggtctt ggcagttatg tccactggga aaagagaata gatgcaaaga tttcatatgt
     5161 tttgatgagt atccccggag ttaagggtat tgaaatagga cttggattcg gaggaagcaa
     5221 attatttggt tctgaattcc atgatgagat tttttataaa aataaagttt acagaaaaac
     5281 aaataattct ggtggcatag agggtggaat aacaaatgga aatgatttat ggataaaatg
     5341 ctttttcaaa ccaataccga ctgttaaaag aggactttta tcttttgata taagggattt
     5401 taaagaaaaa aaatatgaat atgaaagaag tgatatatgc gcagttgttc ctgcctcaat
     5461 aatcggagaa aatttacttg cttttattct cgcctctgaa tatatagaga agtttgctgg
     5521 tgattctctc aatgatttaa aagaaaatta tagaatttat attgaaagaa taaaaaaata
     5581 ctggttttaa aaatctttta ataaatggaa aagaatattg tacttattgg ttttatggga
     5641 acaggcaaaa ctgaggtagg aatattactt gctaagaggt taaatatgaa ttttgttgat
     5701 actgataaat taatagaaga aagagaaaaa gatagaattg taagaatatt tgaagttaaa
     5761 ggagaggaat attttagaga aatagaagaa aaaattattg aagaggtatc aaattataaa
     5821 aattgtgtta ttgcaacagg aggaggtgct gttataagag aaaaaaatta caataattta
     5881 aaaaaaaatg gaattctgat atgtttaaaa gcaacgcctg aagaaattta taaaagaaca
     5941 atatcaaaaa aggatagacc gttactgatg aaatcaagaa atgtaattga aacaattaaa
     6001 gaattgcttg aaatgagaaa accttattat gaaaaggcag attatatact ggatacaact
     6061 aaaaaaccag tagaaataat tgttgaggga ataattgaaa taatcaaaaa agaagatgaa
     6121 aaaaataaaa gttaaacttg gtgaaagcac tcattatatt tatattggag aatcaataga
     6181 aaaaacagga gaaaaaataa gagatggtga atttggggat aaatttgtta ttttaagcaa
     6241 tcaaagagtt tttaatattt atggagaaaa ggtaagaaaa tcaattgaaa aagaaaatag
     6301 aactgtggaa aaaatactta tcaaagaagg agaaagacag aaaaatatca atacagtttt
     6361 aaaaattata aataaacttt ctgatttaca aataagcaga aatgatacaa ttgtaaatct
     6421 tggtggagga gttataagtg atattggtgg ctttgtatct tccatttata aaagaggtat
     6481 taaatatatt acaataccca ctacattact tgctcaggtt gatgcttcaa ttggaggaaa
     6541 aacaggtata aatttgcctt tcgggaaaaa tcttataggt acattttatc aaccatcttt
     6601 tatacttatt gattttaaaa cactttcaac actaccttat aaggaaatca aacagggaat
     6661 tgcagaaata attaaatacg gagttataaa aaatgaaaaa atttttgaaa aacttgaaca
     6721 gataaaaaaa gaagaaattg aaaacaattt tgcatttctg ataatggaga gcataaagat
     6781 taaagtggat atagttgaaa aggatgaaaa agaaacaaag ggtttgagag aaatcctcaa
     6841 ttttggacat acacttggac acgcaattga aatttcagat ataaaaaaat tctcacatgg
     6901 agatagtgtt tctctgggaa tgataggaga aagttatatt gcttataaaa aaggattgtc
     6961 tgataaagaa acctatttaa gaattaaaga ccttgcaaaa aaatttaaat tatcaaattc
     7021 ttttagtaaa ataaattttg aaaaaattct tgaatttatg aaacatgaca aaaaagttag
     7081 aaaaaacaaa ataagattta cattaccgga aaaaataggg aaggtaagtt taggagtcat
     7141 aattgaggaa aatgaaatat taaaaatttt gaaagaactt ttataaggag aaaaaaatga
     7201 aagaacttga aaaaatacga aaacaaatag atgagataga cgaaaaatta attgaactga
     7261 tgaatttaag agcagaactt gtaaaaaaag tttttgaaat aaaaaataaa gaaaataaac
     7321 cattatttga cccatcaaga gaaaaaaaga ttctggaaag attaattaaa aagaataaag
     7381 ggatacttaa accagaagat atagaagtta taatggaaac gatttttaaa gtttatagag
     7441 gaatgtttaa agaaattgtt gtcgcttacc tgggtcctga aggaacattc acacatcaag
     7501 cagcattaaa taaattcgga aaaaaagcaa gatatatatc agcaaagacc atagatgaag
     7561 tatttaaaga agtggaaaaa gaaagagcag attatggggt tgttccaatt gaaaattcaa
     7621 tagaaggtgt tgttacccat actcttgatg ttttcattga aagtgattta aagataactt
     7681 ctgaaatact ccttgaaatt catcattttt tgctctcaaa agaaaattca atagatagta
     7741 taaaaaaatt atattctcat ccacaggcac ttgctcaatg tagaaactgg ataagtcaga
     7801 atttacccga tgcagaaata tatgaaacag aaagtacaac aagtgctgta aaaaaaatat
     7861 tgaatgaaaa aaaatctgct gctattgcat cagaaattgc tgcttcaatg tataacctga
     7921 atattattgc tgaaagaatt gaagatttta gagaaaatgt tacaagattt ttggtggttg
     7981 gaaaaaatta ttctgaaaaa acaggaaatg ataaaacatc tattcttttt tcaataaaag
     8041 ataaaatagg tgctttacac gatatccttg ttccattcaa aaaagaaggt ataaatttaa
     8101 caagaattga atcaagaccg agtaagaaaa aagcatggga atatgtcttc tttgttgatt
     8161 ttaaagggca taaagatgaa ccaaatgtta aaaaagcgct ggaaaaggta aaaaaatcaa
     8221 ctatatttct taaaattctt ggttcatatc caaaagaagg aggataatat ggaagtaaaa
     8281 aataatagaa ggaggtttat atggctgatt taatcataat gaggcagggt gcaactgaag
     8341 aagaaataga aaaagttgtt gaaaaaatag aaaaacttgg atttcaagca catataagta
     8401 aaggaactga aagaacaatt attggaatta tcggagatac aagaagtgtc tctgatgaaa
     8461 cttttcgtat tcttccaggt gtaagtgaag ttataagtat tttaaaagaa tataaacttg
     8521 caagcaggga ttttcatcct tcagataccg tgatagatgt aaatggaatc aaaataggag
     8581 aaggttattt tgttgttatg gctggtccct gttcagtaga aaataaagaa caacttttaa
     8641 ctacaggtaa atatataaag gaaaagggag taaaaatctt aagaggtgct gtattcaagc
     8701 caagaacttc tccctattcc tttcttggat tgggacctga aggattaagg atagtaaaag
     8761 aaataaaaga agagttaaaa ataccggtaa tcaccgaagt tttgtctcct gaagaagttg
     8821 aaattgtaag tgaagtaagt gatattttac aaattggtac aagaaatatg tttaattaca
     8881 gattattaca gaaagttgga agaattaata aacctgtgct actaaaaaga aatttttctg
     8941 ctaaaatctc tgaattttta aactgtgcag aatatatttt aaaagaagga aataatcagg
     9001 taattttatg cgaaagagga gtgagaactt ttgactctga atttacaaga aatattcttg
     9061 atttaagtgc tataccaata ttaaaaaaag aaacacatct acctgttatt gttgacccga
     9121 gccatggaac aggaagaaga gaccttgtta ttccgatgag taaagcagca atagcagcag
     9181 gagcggatgg tcttttaatt gaagtacatc caaaaccaga agaagcactt tctgatggtt
     9241 ttcaatcatt aaatcttgaa atgtttggaa atttaatgga tgaaattact ccatttataa
     9301 agattatgaa aaagaaaatg tgaaatgaat actattgtta gaaaaccaga aaaaataagt
     9361 ggaattattg aactacctgg agataaatca ataacacaca gaagtttaat atttggaagt
     9421 atatcagcag gaaaaacata tattttaaat tattcaaaag gaaaagattg tttatccacc
     9481 ctgaattgtc ttttaaatct tagtgtgaaa atagaggaag ttgaaaatca attagttata
     9541 aatggctgtg aatttaaaga acctgaggat atccttaact gtggaaactc aggaacaaca
     9601 attcgtcttc tttcaggtat tctttctgca aaacctcttt atactgtttt aacaggtgat
     9661 tcttcactta gaaaaagacc aatggataga ataataaaac ctcttacaat gatggaagca
     9721 gaaatttacg gtagagggaa taataaatat gctcctttga caattatagg taaaaattta
     9781 aaaggaataa aatataaaat ggaaataccg tcagcacagg tgaaaagttg tataattctt
     9841 gccacacttt ttgcaaatgg agaaagtata atagaagaat catatcaaac aagagaccat
     9901 acagaaagaa tgcttgaata ttttggtgga gaaatagaaa aaagagggaa taaaataatt
     9961 gttaaaggaa atcagaaatt gaaaggtaaa gaaatatata tccctgggga tttttcctct
    10021 gcctgttatt ttatcgcagg tacaattttg gttcctgatt caaacttaat tataaaaaat
    10081 gtaggtttaa atcctacaag aactggtttt ttaaatgtta ttgaaagaat gggaggaaat
    10141 tttgaaattt taagaaaaga ggaaatttca ggagaacctg taggagatgt aaaaataatg
    10201 tattctgaaa aattaaaagg aaccgagatt ttgcctgaag aagtaccgaa tataattgat
    10261 gaaattcctc ttattgctgt tttaggcagt gttgcagagg ggaaaactat tgttagaggt
    10321 gcaaaagaat taagggttaa agaatcggat agaataaaat caatttcaac tgaattaaaa
    10381 aaacttggag ccaaaattga agaaaaagaa gatggattta tattagaggg tgttaaaaga
    10441 ttaaagggaa caaaagtaaa ttcatggaat gaccacagaa tagccatgtc tcttgtgatt
    10501 gcttctttca taactgaagg agatatagtt atagaaaatt ctgaatgtgt tgatatttca
    10561 tttcctgaat tttttgataa atttaagtat ctcggagtca attttgagtt tatctaaaat
    10621 ttgaaggagg tcaaaaatga atggaattta cttttacaca ccgacaaaaa ttttttttga
    10681 aagaaatggg atagagaaat acttgccact tgtaaagaat tacggaaata aagttcttat
    10741 tgtttccggt aaaaatttca tttataaaag tggtttaatg gataaaataa aaaacatctt
    10801 tgacaaagaa aacattgatt attctgtatt ttcaaatgtt aatccggaac ccgatataga
    10861 aaatgttgaa gaaggtgttg aattatgcaa gaaagaaaat tgtaatgtaa tacttgcaat
    10921 aggtggagga agtgctcttg atgctgggaa atgtattgct gtaatgtcaa aaaatgaagg
    10981 ttctttaaaa aattactttg gagaagtaga atataaaaat aatccactaa cagttattgc
    11041 tgtaccaaca acatgtggaa caggaagtga ggttacaagg tatgctgtta ttgtggataa
    11101 agaagaaaaa acaaaaaaga cagtaagcag tgaaaaaatt atgcctaaaa ttgcgatttt
    11161 agatcctgat ttacttgatt atttaccttc taaacttatt gcagccactg gaatggatgc
    11221 tttctctcat gctgttgaaa gttatcttgc aacaatttca gattatgttt caaaactctt
    11281 ttctcttgag tcattaaaat tgttaaaaga aaatttaaaa agagcagtgg atggagacaa
    11341 agagagtaaa gaaaaagttt ttcttgcttc aacatttgcg ggatatgcaa taaataaaac
    11401 agggacaatt atagttcacg gaattggata ctctctaaca ataaaatata acactcacca
    11461 tggaactgct aattccttat ttttacctta tattcttgaa tttttgaaaa aaaataatta
    11521 taagaaagaa atagaggaac ttgaaaatat tataggaact aattcagaac ttaagaactt
    11581 cataacttca attggacttc cttcaaaatt gagagatatt agagtaaaag aagaggatat
    11641 agaagaactt gttaatttat ccataaaagg atgtgaaagg gctatgaaga agatgaaatt
    11701 tgtattaaca agaaatgatt ttttagaaat aatcaattct gctttttaat tattccataa
    11761 aatatcttta aagacattca aaaatccata ttttctctgc catgtagtat acataaaacc
    11821 aactatatta ttgaattttt ttgatatttc ataccacaat ttaacatcat tcatattatc
    11881 tgcatcataa taacacgcta ttaaaacttt aaatccctga ttataaaaaa attctaaact
    11941 tttttctctt ggtcttccac cccatacagc aataataata tcttttggta tataattcca
    12001 tgaaccagta aaatctcctt taacgagata atagtccttt tttgcattat gatttggatc
    12061 aaacatatct gaccatatat aaatttctgc tttaggatta tatttacgaa gaatttccac
    12121 ttcttttttt acacattcac caagtaattc agccatatct tttcctaaac atgcctcaca
    12181 tgttcctccc attctaattt catccatatt taatagaact tttttataat ttaaagtttc
    12241 ccataataac tttgcttcat ggtcaaaaat ctcataaatt tcaggttcag ccatacaaac
    12301 agttacctga ctatcataaa ttaacatcgg atgataccaa ctaactctta atctctgtcc
    12361 attttttata cgactattct gggttatctt aattttagga aaaggtctgt caaaatttgt
    12421 ccataagtta aaatttttgt ctattaatgg ttcataatct tctccttctt tatatatttc
    12481 tccacttcct tcattcctga cagttatagg agttccaggt cttcttaata catttattgg
    12541 tcctatttct tcaatcatcc agtcatcaag ataaaattta cctgtttttc ctccccagac
    12601 accggcataa acacgaactt tttcaaagtt ctgactgtta aaaatcatag ttaattttct
    12661 ccagtcagtc gtgggtggta aattaaaact tcttggagca aggtcttttt tatctccgag
    12721 aactaataat ctgaaacatc cctgtggttg taaattttct gttttaaccc atatactcat
    12781 acgataacat ctatatggtt taacagaaat ttcctgcatt attcttccat gaccataagg
    12841 atttgcagta aaattctcaa atcttataga agtattacca ctttttttaa tattttgctc
    12901 aacaaaactt acctcacccg gctggtcatg aaaattaaag tatttaaatc tatttccatt
    12961 aaattcttca aaactaccat ttagaatttg aacatttaat tcagagataa aaattgcttc
    13021 tccatttttt acaacaaaaa gagcatcttt aacaggtaaa ccctctgcaa gatatttatt
    13081 atgtgataaa actcctcctc catatccaat agaaaaaact gccgggatca gttcaagatt
    13141 aagtttttca caacattctc taatttcctt aagatttcta aaataccttt cgtctttttt
    13201 gcacaatgaa tcaagtcctg cggaaagtat tactccatta aaatcattct tctttgcgtc
    13261 atttaaaatt ctcttgactt cttccaaatc ttcatttttc tcaatattaa atccaaaaac
    13321 ccaaacaaat ctgtctttaa aattatcaca gagaagaata attgaaacaa aaaagaaaac
    13381 taaaaatggt aatgtttttt tcattagaga tatggttcca gaaatttaat cgcttttttt
    13441 atatcctcaa tctgtttttc accacttatt tccctctcaa ttattattgg tcctttaaaa
    13501 cctttacttt tcaaccttct caaaagtaca ggaaaattaa ccatgccttc accgataggt
    13561 acctccttcc caagatattc atctctattt ggccaaagtc catcctttcc atgcatactt
    13621 tttacatact caccaaaaat ttcaacggca tcaattggat ttgctttccc atataaaatt
    13681 aaatttgccg ggtctaaatt tattccaaca ttatccattc tcatatcaag tattgttctt
    13741 ttcaatgttg atggtaattc ttgtcctgtt tcaaagcaaa aaatctgatt attttcttta
    13801 catttttcaa caactgcctt cattacaggt aaaaaagatt tataaatcgg gtcttcttca
    13861 tcatcaggaa taaaacctac atgacagaca atatttttta ctcctatttc tttaacaaaa
    13921 tcggaaaaat caaaagccag ttttaatctt tcctctctat attttggagc gataaaaccc
    13981 attgtctccg gaccatcttt attattaaaa atctgaccct taaataacat aaaaactgaa
    14041 aaaatttcta cttcataatt ttctgctttc ttttttattt tataagggtc aaacctattt
    14101 atttcttctg aaagaaaccc aatctgacaa agcgataagt taaaggattt cactttttta
    14161 aaactttctt caagatttcc tgaactaact atgagcccaa gtttcaattt attcatattt
    14221 ctcctttctt tatttcttta attacattcc ggaattaatg gtcctgcaag ttctttcagg
    14281 aaaaataatt ttccaggtaa agttggcata aaactgctcg gaacccaaat atcaggacca
    14341 tatcttaaag ttacccataa actcattccc tgccacatca ttcctcttcc aagtgttcca
    14401 tccgctccac ctataatttt atctgctttt aaaaatattc cgggaccaat agtatttgca
    14461 aaacatggaa cgagagttgt tctgctttta aaacttgtaa ttgcattctg ttcaattgtt
    14521 aatggatgta attttgctcc aagtcggtat tcctgttttt tccattcttc ttctgaatta
    14581 aattcttcgg caaaatgtgg ttcccagaaa gcaatatgaa aaactcttcc aattccaaaa
    14641 ataacaacaa gtttagcacc tttttttctt aattcattta ttttttctgg atattttggc
    14701 agtaaatttt ttgttgcaaa aaacctctga tttttcggaa tcgttaattt tccaagaggt
    14761 ccataaaaag catcttccat agcattttga aaagcacccg gctctgttgg aggtaatgta
    14821 tttccttctt tatcactcca ttcatccata ttaaatcccc agacatgttt acaatcaata
    14881 ttccattctt ttaaaaaata aacaacccat ttatacattc ccataggtcc cacaggtaaa
    14941 atcaaaatta aatttcttcc ttcttctttt gctgcacata tttcataggc aatttcatgc
    15001 cccatcttta cattgaattc ctcaaacgta tcacaagaaa ccggttgaaa atccttattc
    15061 caccattttt gtctttctgt tatcttttct ggtggatttg aacaacactt atctattttc
    15121 tttaaatccc atccctttgg aaagaaattt tctaaaagag acccttttat tgtatttatt
    15181 aaattcatat atcccccttt tttgtttata gttttttcat tttaactctc ttgtaaaatt
    15241 tgttttttta ttttatcata aatctaaaag taaaaaaatg aaagaaaata taaaatttgg
    15301 agttattggt gcaggaggaa ttgctacaag aagaacaata cccggtatgc ttgaagcaaa
    15361 aaatattgag gttcttgctg ttatggacat aatgaatgca aaagaaattg ctaaaaaatt
    15421 taatataccc aaatattata aaaaagaaaa agatattgtt aaagaccctg atattaatgc
    15481 agtttatatt gcaagtcctg tttatttaca tttaaagcat ataaaaatgt ctgctgaaaa
    15541 tggaaaacat attttatgtg aaaaaccttt atgcaaaaat ttaaaagaag ggaaagaagc
    15601 ggttagaata tgtaaaaaaa atggtgtttt tattcaggaa ggttacatga tgaaatttca
    15661 tggggcacat aaaaaaataa aagaattaat agaaaaaggg gaacttggaa aaattgtata
    15721 tatgagaggt caactttcat gctggtatcc tcctatagaa ggagcatggc ggcaggttcc
    15781 aaataaaggt ggagggggtt ctttaattga tatggcaaca catttatatg accttcttga
    15841 attttttgtt aatgacagaa ttaaatatat ttcagcaatt gtaaatacac aggttcataa
    15901 ttacaaagtt gaagattctt ccacaactct tatagaattt tcaaagggta cacatgcaac
    15961 agttgactgt tttttctgta ttccagatga atcaagtaaa acaagattag aaatatatgg
    16021 aagtaaagga gcaattctta cagaaggaac aatagggcag agtataggcg gaaaaatgga
    16081 aggaatattt ggacttggag aaagtaacta tgatgctacc cagagtaagg atattgtaaa
    16141 ggaatttaaa gaaattgaat ttgagaaaat aaatccatac actgctgagt gtgaatattt
    16201 tgcagactgt atcttaagag gaaaaaaacc tgaaataaat aactgggaaa acgcccttca
    16261 catacttaaa gttacagaaa aggcatatca atcatacaaa aagaaaaaaa ttatgaaagt
    16321 ttgaataaat gttacagatt atatgaattt gtaaataatt aatcaaccac aggtataaaa
    16381 cactggaatt tgaaaaattc caagtgtttt ttctggcgt
//
LOCUS       DAOSAT010000034        16160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2486_length_16160_cov_5.814157, whole genome shotgun sequence.
ACCESSION   DAOSAT010000034 DAOSAT010000000
VERSION     DAOSAT010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16160
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2486_length_16160_cov_5.814157"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..1865
                     /locus_tag="PLF90_04190"
     CDS             231..1865
                     /locus_tag="PLF90_04190"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOK56512.1"
                     /translation="MEILKPSRNEIPEKGIIIGQGEITEKIFPEETSYLLEKTSSFGG
                     EQIIIKTKGKYLLLAGGRPRGTIYSVYRFLSNICGVRWWTPWATYIPKKKTLKIPHLN
                     IKEKPAFEYREVYWYLALDGNWACRNYCNGHFERLEEKHGGKIKYKGFVHTFYKLCPP
                     SIYFEKKPEWFSLIKGERKIEGGQLCCTNLELRDFLVEKVREYLKETPDANIISISQN
                     DTFAGNCECEKCKEIDEKEGSPSASVLDMVNYIAKKLEKEFPSVAFDTLAYRYTRKPP
                     KFIKPYHNVIVRLCSIECNFAQPLDHESNKSFSEDIYGWSKFTNRIYIWDYTTNFSHY
                     VLPHPNWFSLGQNIRFFHKNGVKGVFEQGAYQSNGAEFAELRSWVLAQLLWNPYQNDK
                     KLIDEFLNGYYGKAGVYIKKYIELIYNSAKNYYLTCYTKPDAPFFNFKVLNEAERLWQ
                     KAEKSVKDNPEIYWRVRLSHMPVLYAFLTNWERLKEECKKQNKKWPLSESKKEVAEEF
                     LKVLIGKGPSNWSPLTHLNEGGLTPQDFVSSIIAKE"
     gene            1992..2660
                     /locus_tag="PLF90_04195"
     CDS             1992..2660
                     /locus_tag="PLF90_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56513.1"
                     /translation="MDVNYFWNPESKEIYFDQYDWSSKVKDFLEMGVDKIEKISFEPI
                     DLSKYLNINLEKGILEETKGVVLPEKISFQKIPFIIQKNSGVILRPGNEISIEIDKKC
                     KYLYFLHLSTLPMSIDAYYSPIISLKKPKIGNYKIIYKDGTSVDIPIINRLNIIHWNS
                     RFSPYFCRVAYQTKDIDRNLFRVFFYKFKNPYPEKKIDKIEIAYIENEEKSLPIILGL
                     TAGL"
     gene            2864..4168
                     /gene="hcp"
                     /locus_tag="PLF90_04200"
     CDS             2864..4168
                     /gene="hcp"
                     /locus_tag="PLF90_04200"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOK56514.1"
                     /translation="MDELKMFCYQCSQISQGNGCVNYGVCGKSPTLSRLQDNLIFGIK
                     GISAYLYHANELGYRDKELEKFLIETLYSTLTNVNFDTERFIKLALDVGKINIETMRL
                     LKKAHIETYGEPQPVEVFTGTKKGKGIIVTGHSLKVLEEILKQAEKENINVYTHSELL
                     PAHGYPKIRKHENLIGNLGKAWYDQRKLFSEIPACIVGSSNCVLIPKEDYKDRFFTTG
                     VVGLPGVKHITTYDFSEVIEKAKTLPDLPERNGENVLITGFSLSTILSSKDKIKELIE
                     KGKIRRIFIIGGCDNPTNRNDYFRKLAQILPENTIIITLACGKFRINDLDLGDIDGIP
                     RLIDIGQCNDAIIGIDILTSLAENFGVNDLNELPVSYFFMWMEQKAVAILWSLLYLGI
                     KGIRIGPILPAWINEEILRALVKNFDLKIIDEPEKDLKEILK"
     gene            4178..5002
                     /locus_tag="PLF90_04205"
     CDS             4178..5002
                     /locus_tag="PLF90_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK56515.1"
                     /translation="MKRKIIEIDEEKCTGCGDCIPNCPEGALQIIDGKARLISDLFCD
                     GLGACIGSCPVDAIKIVERDAEPYDERKTMENIIKKGKNTIIAHLKHLHEHGETEYLR
                     QALEVLKEKNIEIDLNEIIEVPCCPGMKVVEMKKEEEEGEENISVKSQLSQWPIQLHL
                     INPLAPYFKKADLLIAADCTSYAYGNFHLDFLKGKKLIIACPKLDTGIDRYIEKIKQL
                     VEVADINTITVVIMEVPCCLGLLKIVEEGIKKSNKKVPIKKVVISIKGEILEENWI"
     gene            4999..5229
                     /locus_tag="PLF90_04210"
     CDS             4999..5229
                     /locus_tag="PLF90_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56516.1"
                     /translation="MKCPGTDPRFLKVEVKKCPKCGYEVEIFSDEVRVKCPKCKNYVY
                     REIPSCIDWCKYAKECIGEKKWKELVEKNGKE"
     gene            5216..5914
                     /locus_tag="PLF90_04215"
     CDS             5216..5914
                     /locus_tag="PLF90_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOK56517.1"
                     /translation="MEKNKSGFTVIEILVVISIITILFSIILPLLKKAREKARQAVCM
                     NNLRQISIALSLYIDDYDGYFPCADDPLSISPYYWLWMGRGWRKLLTPYIKKSITEKD
                     PSILYCPSDRTAPIKWESTSYGYSMSFYHSPEQINMMTDPSYTYDINKIMPSIGQKSS
                     KVKYPDKKIVIAEWLDNHTGTNSGWWSWKGSRNYLFVDGHVEFLKAEKILPANDGYPD
                     INLTKDGILGKDIE"
     gene            6030..6479
                     /locus_tag="PLF90_04220"
     CDS             6030..6479
                     /locus_tag="PLF90_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOK56518.1"
                     /translation="MRFSTKTRYGLRALIDLGIYYEGKPVFVKDIAKRQNLSERYLEH
                     IMLTLKKAGILRSIKGGKGGYAFLKDPSEIRVREIVEILEGTISPVECVEKKEICGRS
                     ENCVARELWSRIKEEILKFLDGITLKELIEKQKEKNEKKEKTVFYEI"
     gene            6491..7405
                     /gene="cysK"
                     /locus_tag="PLF90_04225"
     CDS             6491..7405
                     /gene="cysK"
                     /locus_tag="PLF90_04225"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOK56519.1"
                     /translation="MMKVAENITELIGDTPLVKLNRIKKGIDAEIYAKLEFYNPCGSV
                     KDRIAVSMIEDAEKKGIINKDTIIIEPTSGNTGIGLAFVCAQRGYKLILTMPESMSIE
                     RRKLLSLFGAEIVLTPADEGMTGAVKRAEELLKKYKNSFMPQQFKNPANPEIHRKTTA
                     EEIWRDTEGKIDIFVAGVGTGGTITGVGEVLKQRKKDLKIVAVEPENSPVLSGGTPGR
                     HKIQGIGAGFIPDVLNLKIIDEIIKVSDEDAIETAKRLAREEGILCGISSGAAVYASL
                     IVGKRNENSGKIIVVILPDTGERYLSVF"
     gene            7418..8179
                     /locus_tag="PLF90_04230"
     CDS             7418..8179
                     /locus_tag="PLF90_04230"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010885445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HOK56520.1"
                     /translation="MEKIITEGIVKSFLNEFLKNTEVDVVISGAGPSGLVCGYYLSKN
                     GIKTVIFERHLKVGGGMPGGGMMFNKIVIQEEAVDILKEFKIKVKKFRDKFYVADAIE
                     TISGLTYRTVRSGAKIFNLISVEDVVIRENRVNGVVINWTATEIAKFHVDPLAIISKY
                     VVDATGHDCEICKIVEKKVGGISVKGEKSMWAEIGEKEIIENTKEVYPGLIVCGMAAN
                     AFFGSPRMGAIFGGMFLSGKKTAEIILNFLQGGRR"
     gene            8176..9369
                     /locus_tag="PLF90_04235"
     CDS             8176..9369
                     /locus_tag="PLF90_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOK56521.1"
                     /translation="MKTIKEINERIKKGEAVVVTADELVELKKSANTKKLAEEIDVVT
                     TGTFGPMCSSGVFLNFGHSKERIKIGGGKVYLNGVPAYSGLAAVDVYIGATALPDDDP
                     RNSKYPGEFKYGGGHVIEDLVSGKEIELVIYTYGTDCYPKKYLKTKITLNDINEAILF
                     DPRNCYQNYNVAVNLSNRTIYTYMGVLKPNLGNANYCSAGQLSPLLKDPYLKTIGFGT
                     RIFLGGGIGYVAWWGTQYNPGVKRRENGIPMAGGATLSLIGDLKQMKSEFLKGASLTG
                     YGTSLIVGIGIPIPILNEDILNWTSIPDEEIYAPIVDYSYDYPHSTGKVLDYVNYGQL
                     KSGRIKIMGKEVSTGSLSSYKKAKEIAEILKNWIKNGEFLLTEPVFYFPGPESAYKFK
                     PMKEV"
     gene            9371..9775
                     /locus_tag="PLF90_04240"
     CDS             9371..9775
                     /locus_tag="PLF90_04240"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOK56522.1"
                     /translation="MEKKFVMHFPPVLVDKPMISTIVKKYNLDFNILKAYVSPEEEGT
                     LVIAFSGEEKNVFDAVEELKEKGVKVQLIESDIKMIRERCTDCSVCVALCPVGALSLN
                     RETFEVNFDPEKCIACELCIKGCPTEAMISTI"
     gene            9814..10977
                     /gene="nifS"
                     /locus_tag="PLF90_04245"
     CDS             9814..10977
                     /gene="nifS"
                     /locus_tag="PLF90_04245"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOK56523.1"
                     /translation="MEMIYMDCNATTPTDERVLKEMLPYFSEKFGNPSSIYEIARENK
                     KEIEKARENVAKLIDAEPDEIYFTSGGTESDNWAIKGIAFVLRNKGNHIITSQIEHHA
                     VLNTCKFLSKIGYEITYAPCDKYGIIDIDFIRKAIKKETILITIMHANNEIGTIEPIE
                     EISKIAKENDIYFHTDAVQTVGKIPVSVKKLGVDMLSISGHKFYGPKGVGAFYIKKGT
                     KIETFINGGEQEKGKRGGTYNVPGIVGLGKAAEIAMEEMEEEIKKVKYLRDKLENGII
                     ERIPEIVINGHPENRLYNTLNLCVKYIEGESILLNLDFEKIYASSGSACTSGSLEPSH
                     VLLAIGLPHEIAHGSLRFSLGKYNKEEDVDKVLEILPKVVEKLRKLSPFWEKK"
     gene            10974..11870
                     /gene="nadA"
                     /locus_tag="PLF90_04250"
     CDS             10974..11870
                     /gene="nadA"
                     /locus_tag="PLF90_04250"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOK56524.1"
                     /translation="MIEEILKLKKEKDAIILAHNYQIPEIQDIADFVGDSLELARKAS
                     QIEAKIIVFCGVKFMAETAKILSPDKKVLLPDLDAGCPLTDMAKYEDVLKIKEKYPDA
                     WVVSYVNTNAIVKAITDVCCTSANADKVVRNVPAKKIIFLPDKNLCWYVKQKVPDKEI
                     ICWDGYCFVHRLFKAEDVIKAREKYPDSEIIVHPECDPEVQKLADGIYSTSGMLKRAK
                     ESKAKIMIIGTEEGLLHRLKKENPEKEFYSLGSSKICPNMKKITIEKLYKSLKEEIYE
                     INLKQIIIEKGKKSLERMLEYL"
     gene            11909..12307
                     /locus_tag="PLF90_04255"
     CDS             11909..12307
                     /locus_tag="PLF90_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOK56525.1"
                     /translation="MEYTEKVLEYFRNPRNMGRIENADGIGKVGNPVCGDVMWIYIKV
                     GKNEKGEEIIEDIKFETFGCGAAIATSSVVTELAKGKTIEEAEKITNKEVVEFLGGLP
                     PVKKHCSLLAEEGLKAAIEDYKRKKNERKG"
     gene            12291..12680
                     /locus_tag="PLF90_04260"
     CDS             12291..12680
                     /locus_tag="PLF90_04260"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOK56526.1"
                     /translation="MKEKGKIIESKEDKALVLMEEKEKCKSCGLCKKIIPRQPVIEVE
                     NIIKAEVGDNVEIEIKEDDLFEVSIYIYGFPLLGFILGIISAYFLNNMFLKVIIFLLF
                     LISFWVFGFRKGRIYGERAKPKIISKI"
     gene            12640..13737
                     /locus_tag="PLF90_04265"
     CDS             12640..13737
                     /locus_tag="PLF90_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56527.1"
                     /translation="MEKEQNQKLYLKYKDDFEEAKKYWMAFWKGEIIDRPLLILSFPK
                     DENKKVSGPSYLSGMDGDFEKPISQFEEFCENTIFFAESIPSLNISFGPDTVAYCVKG
                     ASLNVRIKSQTAWIEPFVDDWDKFKKIEIEKDGEWYKKFICFYRYASERGDDKFLLQM
                     PDFHTHIDLLRAIRGSENLCIDLIERPDFIKKLLDEIKDVFIDIYTEIYSSTDMKKWG
                     TTCWIPFYSEKKYCTIQCDFICMISPEMFRKFALPYIEYETDFLDHSIFHLDGPGALK
                     HLDDLLSIKKLNAIQWVEGAGNKPHIYWIDLFKKIIKAGKSVVIYPRSFEEIKVFHKE
                     LGPKKIVYQPAWQIKNLKEAEEIKGWLIKNS"
     gene            13747..13977
                     /locus_tag="PLF90_04270"
     CDS             13747..13977
                     /locus_tag="PLF90_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOK56528.1"
                     /translation="MDLKEKVENSIKDVREFLQAEGGDCEIVEAKDGIVKVRLIGTCK
                     GCPFSQITLKMRIEAIIKDRVPEVKEVVEVEE"
     gene            13977..14774
                     /gene="larE"
                     /locus_tag="PLF90_04275"
     CDS             13977..14774
                     /gene="larE"
                     /locus_tag="PLF90_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOK56529.1"
                     /translation="MEEKLKKLKDYLKKLKRVVIAYSGGVDSTFLLNVALNTLGKENV
                     IAVIGISPTYPNEERKFAVNFCKENGIKYIEVETEEFKDSNFLKNPPDRCFWCKKELF
                     GKIEEIRKNLNFKYIIDGTNKDDEKDYRPGEKAKKIYNVISPLKECGFTKKDIRELSK
                     ELNLPTWNKPQMACLASRIPYGEKITEEKLKRINEGEKFLYSLGFKIVRIRDYNELAR
                     IEVDKNEIEKLIKFKEKIIEKLKKIGYKYITVDLEGYRTGSMNEVLR"
     gene            complement(14766..>16160)
                     /locus_tag="PLF90_04280"
     CDS             complement(14766..>16160)
                     /locus_tag="PLF90_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK56530.1"
                     /translation="ITDGFIIFLMSIFTFFGAFFILLKLNKVLTLLMMFPIGGIGFLV
                     YVFTEKAHKGYREVRKIRGELTSSLQENIFGMKEIKAYAREKHQLAKFSFKGRKFFKI
                     NLYVAKLWATYQSWIIFLSSLGYLIIIWFGSIKVVSGEISVGTLVAYIGYLHLLYTPV
                     HQLNQVNHMMQHARAAGERIFEIMDAYSDVKDSPNSIFPSTNLKGKVKFENVYFAYED
                     EKYVLKNISFDVKEGEKIALVGPSGAGKSTLVSLIPRFYDVNKGSIYIDDKNIKDYKI
                     FYLRSQIGIVLQEPFIFSGTVMENITFGKKNVSENEVIEAAKLANAHDFISNLPDGYE
                     TDVGDMGNSLSVGQKQRIAIARVFLKNPSILILDEFTSSLDAESESLIMEALQRLIEG
                     RTVFIIAHRLSLVRNVDRIFVLNEGEIVETGNHLQLLKEKGLYYKLYNLQYPLEKIEK
                     EINELSKIKMGLSP"
BASE COUNT         6209 a         1902 c         3066 g         4983 t
ORIGIN      
        1 acactttgac aaatttaaaa aataaattaa aataaaattg gaattggatt tacagacaaa
       61 attttaaaaa aggggataaa atagcaaaaa agagtaaaaa aagatatatc tatcttactc
      121 attcaggtaa agcaaaaatc cctgttataa ttcagaaaga agcaactgaa tcagaaattt
      181 atgcaggaga agaacttgca gaatatattt ttaaaattac aggtgcaaag ttggaaattc
      241 taaaaccatc cagaaatgaa attccagaaa aaggaattat tataggacag ggagaaatta
      301 cagaaaaaat tttcccagag gaaacttctt atcttttaga aaaaacttct tcatttggtg
      361 gtgaacagat tattataaaa acaaaaggaa aatatttatt acttgctggt ggaagaccga
      421 gagggaccat ttattctgta tatcgttttt taagcaatat ctgtggagta agatggtgga
      481 caccatgggc aacatatata ccgaaaaaga agacattgaa aatcccccac ttaaatatta
      541 aagaaaaacc agcatttgaa tacagagaag tatattggta tcttgcactt gatggtaact
      601 gggcatgcag aaattattgt aatggacatt ttgaaagatt ggaagaaaaa catggtggaa
      661 aaataaaata taagggtttt gtccatacct tttacaaact ctgtcctcca tcaatttatt
      721 ttgagaaaaa acccgaatgg ttcagtttaa ttaaaggaga aaggaaaata gagggtggac
      781 aactctgctg tacaaattta gagttaagag attttcttgt agaaaaggta agggaatacc
      841 ttaaagaaac acctgatgca aatattattt ctatttctca aaatgatact tttgcaggta
      901 attgtgagtg tgaaaaatgc aaagagattg atgaaaagga aggttctcca tctgcctcgg
      961 ttcttgatat ggtaaattac attgctaaaa aacttgaaaa agaatttcct tctgttgctt
     1021 ttgacacact tgcttataga tatacaagaa aaccaccaaa atttataaaa ccttaccata
     1081 atgtaattgt tcgtctctgt tcaattgaat gtaattttgc tcaacctctt gaccatgaaa
     1141 gtaataaatc attttctgag gatatttatg gatggtcaaa attcacaaat agaatataca
     1201 tatgggacta tacaacaaat ttttcccatt atgttttacc gcatccaaac tggttttctc
     1261 ttggtcaaaa tataagattt ttccataaaa atggagttaa aggagttttt gagcagggtg
     1321 catatcaatc gaatggagca gaatttgcag aacttcgttc atgggttctt gctcaacttt
     1381 tatggaatcc atatcagaac gacaaaaaac ttattgatga atttttaaat ggatattatg
     1441 gaaaggcagg tgtttacatt aaaaaataca tagaacttat ttacaattca gcaaaaaatt
     1501 attatctgac atgctataca aaaccagatg ctccattttt taattttaaa gttttaaatg
     1561 aagcagaaag attgtggcag aaagcagaaa aatctgttaa agataatcct gaaatttact
     1621 ggagagttcg tctttcccat atgcctgtat tgtatgcttt tttaacaaac tgggaaagat
     1681 taaaagaaga atgcaaaaaa caaaataaaa aatggccctt atctgaatca aaaaaagaag
     1741 ttgccgaaga attccttaaa gttttaatag gaaaaggtcc ttctaactgg agtcctctta
     1801 cacatttaaa tgaaggcggt ctaactcctc aagattttgt ttcttcaata atagcaaaag
     1861 aataatccca cctttaaggt ggacaatggg aaagaaggca ggacaaggga tttaagaggg
     1921 ggaaaattta tacttttaac tatataacaa catcttatac ttggtgtgag agtgtctggt
     1981 ctgttatagg aatggatgta aattattttt ggaatccaga atcaaaagaa atttattttg
     2041 atcaatatga ctggtcaagc aaagttaagg atttcttaga aatgggagta gataaaattg
     2101 aaaaaataag ttttgagcca attgatttat caaaatattt aaatataaat ttagaaaaag
     2161 gaattttaga agaaacaaaa ggtgtagttt tgccagaaaa aatcagtttt caaaaaattc
     2221 catttattat tcagaaaaat agtggagtta ttctacgacc aggaaatgaa atatctatag
     2281 aaatagataa aaaatgtaaa tatctgtatt ttttacatct ttcaacttta ccaatgtcta
     2341 ttgatgctta ctattcacca attatttcct taaaaaagcc caaaattggt aattataaaa
     2401 taatttataa agacggaaca agtgttgata ttcctataat aaatagatta aacataatac
     2461 actggaattc aagattttct ccttattttt gccgtgttgc atatcaaaca aaagatatag
     2521 atagaaattt atttagagtg ttcttttata aatttaaaaa tccttatcca gaaaaaaaga
     2581 tagataaaat tgaaattgct tatattgaaa atgaagaaaa atcattgcct attatcttag
     2641 gattgactgc aggtctataa atttaacctc cgaacccggt tcggaaggtc ctgggtatta
     2701 ttatcccttc taaatatggt ggtgttttat tcggtgaatt cttttgcctt cttgataaat
     2761 ccttgatact atgtcttaac ctaaaagaga aaatatattg acaaaaaatt tttttattga
     2821 tataatatta aaatgataat aaaagtaata aaagggagaa aaaatggatg aattaaaaat
     2881 gttttgttat caatgtagtc agataagtca aggtaatgga tgtgtaaatt atggtgtatg
     2941 tggtaaaagt ccaactcttt caagattaca agataatttg atttttggga ttaagggaat
     3001 aagtgcttat ttatatcatg caaatgaact tggatatagg gataaagaac ttgaaaaatt
     3061 tcttatagaa accctttatt caactttaac aaatgtgaat tttgatacag aaagatttat
     3121 aaaacttgct cttgatgttg gaaaaataaa tatagaaaca atgagattac ttaaaaaggc
     3181 acatatagag acatatggag aaccacagcc agttgaggtt tttacaggga ctaaaaaagg
     3241 taaaggaatt attgtaacag gacacagttt aaaagttctt gaggagattt taaaacaggc
     3301 agaaaaagaa aatattaatg tatatactca ttcagaactt ttacctgcac atggatatcc
     3361 taaaataaga aaacatgaaa accttatcgg aaatcttggc aaagcatggt atgaccaaag
     3421 aaaacttttt tcagaaattc cagcatgtat tgttggaagt tcaaattgtg tattaattcc
     3481 aaaagaagat tataaagaca gattttttac aacaggagtt gttggacttc cgggtgttaa
     3541 acatataact acttatgatt tttctgaagt tattgaaaag gccaaaacat tgcctgattt
     3601 accggaaaga aatggtgaaa atgtcttaat aactggtttt tctctttcaa ccattctctc
     3661 ttcaaaggat aaaattaaag aacttataga aaaaggcaaa attagaagaa tttttataat
     3721 aggtggatgt gataacccaa caaatagaaa cgattatttt agaaaacttg cccagatttt
     3781 gcctgaaaat acaattataa taactcttgc ttgtggtaaa tttagaatta atgacttaga
     3841 ccttggagat attgatggaa tcccaagatt gatagatata ggacagtgta atgatgcaat
     3901 tattggaata gatattttaa cttcattagc agaaaatttt ggtgtaaatg acttaaatga
     3961 acttcctgtt tcatattttt ttatgtggat ggaacaaaaa gcagttgcga ttttatggag
     4021 tttactttat cttggaataa aagggataag aataggacca attcttcctg catggataaa
     4081 tgaggagata ttgagggctc ttgttaaaaa ttttgattta aagattatag atgagccaga
     4141 aaaggactta aaagaaattt taaaatagga gggaaaaatg aaaagaaaga tcattgaaat
     4201 tgatgaagaa aaatgtactg gatgtggaga ttgtattcca aattgtccag aaggtgcttt
     4261 acaaataatt gatggaaaag cacgacttat aagtgattta ttttgtgatg gactcggtgc
     4321 atgcattgga agttgtccag ttgatgcaat aaaaatagtt gaaagagatg cagagccata
     4381 tgatgagaga aagacaatgg aaaatataat aaaaaaaggg aaaaatacaa taatagcaca
     4441 tttaaaacat cttcatgagc atggtgagac agaatattta agacaggcac ttgaagtttt
     4501 gaaagaaaaa aatattgaga ttgatttaaa tgagattatt gaagttccat gttgtcccgg
     4561 gatgaaagtt gttgagatga aaaaagaaga ggaagaagga gaagaaaata taagtgtaaa
     4621 atctcagtta agccagtggc caattcaact tcaccttata aatcctcttg caccatattt
     4681 taaaaaggcg gatttactta ttgctgctga ttgcacttcc tatgcctatg gtaatttcca
     4741 ccttgacttt ttaaaaggta aaaaattgat aattgcatgt ccaaaacttg atacaggaat
     4801 tgatagatat atagaaaaaa ttaaacaact tgttgaggtt gcagatataa atacaattac
     4861 agtagttatt atggaagtcc cttgttgctt gggtctttta aaaattgttg aggagggtat
     4921 aaagaaaagt aataagaaag tccctattaa aaaagttgtt ataagtataa aaggtgaaat
     4981 attagaggaa aactggatat gaagtgtcct ggaactgacc caagatttct aaaggttgaa
     5041 gttaaaaaat gtccaaaatg tggatatgaa gttgagattt tttcagatga agtcagagtt
     5101 aaatgtccta aatgcaaaaa ttatgtttat agagaaattc cttcatgtat tgactggtgt
     5161 aaatatgcaa aagaatgtat tggtgagaaa aaatggaaag aacttgttga gaaaaatgga
     5221 aaagaataaa tcaggtttta ctgtcattga aattctggta gtaatatcaa taataacaat
     5281 actcttttct attattttac ctcttttgaa aaaagcaaga gaaaaagcaa gacaggcagt
     5341 atgtatgaac aatttaagac aaatatcaat tgctttaagt ttatatatag atgattatga
     5401 cggctatttc ccttgtgctg atgacccttt aagtatttct ccttattact ggttatggat
     5461 gggaaggggc tggcgaaaac tattaactcc ttatataaaa aaatcaatta cagaaaaaga
     5521 cccaagtatt ttgtactgtc cgagtgatag aactgctcca attaaatggg aaagtacgtc
     5581 ttatggatat tcaatgagtt tttaccattc tccagaacaa ataaatatga tgacagaccc
     5641 ttcttatact tatgatataa acaaaattat gccttctatt ggtcaaaaat caagtaaagt
     5701 aaaatatcct gataaaaaga tagttatagc agaatggctt gacaatcata caggaacaaa
     5761 tagtggatgg tggtcttgga agggaagtag aaattatttg tttgttgatg ggcatgtaga
     5821 atttttgaaa gcagaaaaga ttttaccagc aaatgatggt tatccagata taaatttaac
     5881 aaaagatggt attttaggaa aagatataga atgaaacctt ttgattttca atgtttacca
     5941 ttggacacct cgtaggtgaa cttgacaaaa aatttattga gggtataata ttataaaaat
     6001 gataatgaaa gtaataaatg gagggaaaaa tgaggttttc tacaaagaca agatatggat
     6061 taagagcatt aattgacctt ggaatttatt atgaggggaa accagttttt gttaaagata
     6121 ttgctaaaag acaaaattta tcagaaaggt atcttgaaca tattatgctt acattgaaaa
     6181 aagcaggaat tttaagaagt ataaaaggtg gtaaaggtgg atatgcattt ttaaaagacc
     6241 catctgaaat aagggtaagg gaaatcgttg aaattcttga aggtacaata tctcctgttg
     6301 aatgtgttga aaaaaaagaa atatgtggaa ggagtgaaaa ttgtgttgca agggaattat
     6361 ggagcaggat aaaggaggaa attttaaagt ttcttgatgg aataacatta aaagaactga
     6421 ttgaaaagca aaaagaaaaa aatgagaaaa aagaaaagac agttttttat gaaatttgaa
     6481 aaaggagatg attatgaaag ttgcagaaaa tattacagaa ttgatagggg atacgcctct
     6541 ggtgaaattg aacaggatta aaaagggaat agatgcagaa atttatgcaa aacttgagtt
     6601 ttataatcca tgtggttctg ttaaggatag aattgctgtt agtatgatag aggatgctga
     6661 aaaaaagggg attataaata aggatacaat tataattgaa ccaacaagtg gaaatacagg
     6721 aattggactt gcttttgtat gtgcacagag aggatataag ttaattttaa caatgcctga
     6781 aagtatgagt atagaaagaa ggaaattgtt atctctgttt ggagcggaaa ttgttttaac
     6841 tcctgctgat gagggaatga caggtgctgt taaaagagca gaagaattac ttaaaaaata
     6901 taaaaattca tttatgcccc agcagtttaa aaatccagca aatcctgaaa tacacaggaa
     6961 aacaacagct gaagaaatat ggagggatac agagggaaag attgatatat ttgtggcagg
     7021 tgtaggaact ggaggaacta taaccggcgt aggagaggtg ttaaaacaaa ggaaaaaaga
     7081 tttaaagatt gttgctgttg aaccagaaaa ttcgcctgta ctttcaggag gaacccctgg
     7141 aagacataaa attcagggaa ttggagcagg ttttatcccg gatgttttga atttaaagat
     7201 aattgatgag ataataaagg tatctgatga ggatgcaata gaaacagcaa aaagattagc
     7261 gagagaagag ggtattttat gtgggatttc ctcaggggct gctgtttatg cttctttaat
     7321 tgttggtaag agaaatgaaa atagtggtaa aataatagtt gttattcttc ctgatacagg
     7381 tgaaagatat ttatcagttt tttaaatgga ggaatttatg gaaaagatta ttacagaagg
     7441 aattgtaaaa agttttttaa atgagttttt gaaaaatact gaagttgatg ttgttatttc
     7501 aggagcaggt ccgagcggtc ttgtatgtgg atattactta tcaaaaaatg gaataaagac
     7561 agttattttt gagagacatt taaaggttgg gggtggaatg cctggtggag ggatgatgtt
     7621 taataaaata gttattcagg aagaagcagt tgatatttta aaagagttta agataaaggt
     7681 taaaaaattt agagataaat tctatgtagc agatgcaatt gagacaattt caggattaac
     7741 ttacagaact gtaagaagtg gagcaaaaat attcaattta ataagtgttg aggatgttgt
     7801 aataagggaa aatagagtaa atggggttgt tataaactgg actgcaacag aaattgcaaa
     7861 gtttcatgtt gacccacttg ctataatttc aaaatatgtt gttgatgcta caggtcatga
     7921 ctgtgagata tgtaagattg ttgaaaaaaa agttggtgga ataagtgtta aaggagaaaa
     7981 gtcaatgtgg gctgaaattg gagaaaagga aattattgaa aatacaaaag aagtttatcc
     8041 aggacttatt gtatgtggta tggctgcaaa tgcatttttt ggttctccaa gaatgggagc
     8101 gatatttgga ggtatgtttt tatcaggtaa aaagacagca gaaattattt taaattttct
     8161 acagggaggc aggagatgaa aacaattaaa gagataaatg aaagaataaa aaaaggagaa
     8221 gcagttgttg ttacagcaga tgaacttgtt gaattaaaaa agtcagcaaa tacaaaaaaa
     8281 ctggcagaag aaatagatgt tgtaactact ggaacttttg gaccaatgtg ttcttcaggt
     8341 gtatttttaa atttcgggca ttcaaaggaa aggataaaaa tcggtggagg gaaggtttat
     8401 ttaaatggtg tacctgctta ttctggactt gctgctgttg atgtttatat tggtgctact
     8461 gctttaccag atgatgaccc gagaaactca aaatatcctg gagaattcaa atatggagga
     8521 ggacatgtta ttgaagacct ggtaagtgga aaggaaattg aacttgttat ttatacctat
     8581 ggaactgact gttatccaaa aaaatactta aagactaaaa ttactttgaa tgatataaat
     8641 gaggcaattc tttttgaccc gagaaattgt tatcagaatt ataatgttgc tgtaaatctt
     8701 tcaaacagga caatctatac ttatatgggt gttttaaaac caaatcttgg gaatgcaaat
     8761 tattgttcag caggtcaatt atctccatta ttgaaagacc catatttaaa gacgattgga
     8821 tttggaacaa ggatttttct tggtggaggg attgggtatg tggcatggtg ggggactcaa
     8881 tataatccag gtgtaaaacg aagggaaaat ggaataccta tggcaggtgg agcaacttta
     8941 tctttaattg gtgatttaaa acagatgaaa agtgaatttt taaagggagc aagtttaact
     9001 ggttatggaa caagtttgat tgttggaatt ggaatcccta tacctatttt aaatgaggat
     9061 attttaaact ggacgtcaat acctgatgaa gaaatttatg cacctattgt tgattacagt
     9121 tatgactatc cccattcaac tggaaaagtt cttgattatg tcaattatgg acaacttaaa
     9181 agcggaagga taaaaataat gggaaaagaa gtttcaactg gttctctttc aagttataaa
     9241 aaggcaaaag aaatagcaga gattttaaaa aactggatta aaaatggaga atttctttta
     9301 acagagccag ttttttactt tccaggacca gaatcagcat acaaatttaa accgatgaag
     9361 gaggtatgaa atggaaaaga aatttgttat gcattttcca cctgttcttg ttgataaacc
     9421 aatgatttca acaattgtaa agaaatataa tcttgatttt aatattttga aagcttatgt
     9481 atcaccagaa gaagaaggaa cccttgtaat tgctttttca ggtgaagaga aaaatgtctt
     9541 tgatgcagta gaagaattga aggaaaaagg agttaaggtt caacttatag aaagtgatat
     9601 aaagatgata agggaaagat gtacagattg ttctgtgtgt gttgctttat gtccggtagg
     9661 tgctttatca ttgaatagag aaacatttga agttaatttt gaccctgaaa aatgtattgc
     9721 ttgtgaatta tgtatcaagg gatgtccaac agaagcaatg atatcaacaa tttagcattc
     9781 tcaaactttg cgaaccgggt ttgcgaaaaa ggagtggaaa tgatatatat ggattgtaat
     9841 gcgacaactc caactgatga aagggtttta aaagagatgt taccgtattt ttctgagaaa
     9901 tttggcaatc caagttcaat ttatgaaata gcaagggaaa ataaaaaaga aatagaaaag
     9961 gcaagagaaa atgttgcaaa attgatagat gctgaaccag atgagattta ttttacatct
    10021 ggggggactg aaagtgataa ctgggctata aaaggaattg cttttgtttt gagaaataaa
    10081 ggaaatcata taatcacaag tcagatagaa caccacgcag ttttgaatac atgtaaattt
    10141 ttaagcaaaa ttggatatga gattacctat gcaccatgtg ataaatacgg tattattgat
    10201 attgatttta taagaaaggc gattaaaaaa gaaacaattt taataacgat aatgcatgca
    10261 aataacgaaa taggaacaat tgaaccaata gaagagattt caaaaattgc aaaagaaaat
    10321 gatatatatt ttcatacaga cgcggttcag acagtcggaa aaatccctgt ttcagtaaaa
    10381 aaacttggag tagatatgct ttctatttca ggtcataaat tttacggtcc aaaaggagtt
    10441 ggtgcttttt atattaaaaa gggaacaaaa atagaaacat ttattaatgg aggagaacag
    10501 gaaaaaggta aaagaggtgg aacttataat gtgcctggaa tagttggact tggtaaagca
    10561 gcggaaattg caatggaaga aatggaagaa gaaattaaaa aagttaaata tttaagagat
    10621 aaacttgaaa atggaataat tgaaagaata cctgaaattg ttataaacgg acaccctgaa
    10681 aatcggcttt ataatacttt aaatctctgt gttaaatata ttgaaggtga aagcatactt
    10741 ttaaaccttg attttgaaaa aatttatgcc tcaagtggtt ctgcatgtac ttctggttct
    10801 cttgagccaa gccatgtttt actcgcaatt ggtcttccac atgaaattgc tcacggctct
    10861 ttaagatttt ctcttggtaa gtataataaa gaagaagatg tagataaagt ccttgaaata
    10921 ttaccaaaag ttgtagaaaa attaagaaaa ttaagtccat tctgggagaa aaaatgatag
    10981 aagaaatttt aaaattaaaa aaagaaaagg atgctataat acttgctcat aactatcaga
    11041 tacctgaaat tcaggatatt gctgattttg ttggggactc tcttgaactt gcaagaaagg
    11101 caagccagat tgaagcaaaa attattgttt tctgtggcgt aaaatttatg gcagaaacag
    11161 caaaaattct ttcacctgat aaaaaagttt tattacctga ccttgatgct gggtgtccac
    11221 tgacagatat ggcaaaatat gaagatgttt taaaaataaa agaaaaatat cctgatgcat
    11281 gggtagtttc ctatgtcaat acaaatgcta ttgttaaggc aataactgat gtttgctgta
    11341 caagtgcaaa tgctgataaa gtggtaagga atgtaccggc taaaaagatt atatttttac
    11401 ctgataagaa tttatgctgg tatgttaagc agaaagttcc cgataaagaa ataatatgct
    11461 gggatggata ttgttttgtt catagattat ttaaggcaga ggatgttata aaggcaaggg
    11521 agaaatatcc agattcagaa ataattgttc atcctgaatg tgaccctgag gttcagaaac
    11581 ttgcagatgg tatttattca acatcaggta tgttaaaaag agcaaaggaa tcaaaggcaa
    11641 aaattatgat tatagggaca gaagaagggc ttttacacag attgaaaaaa gaaaatccag
    11701 agaaagaatt ttattctctc ggttcatcaa aaatttgtcc caatatgaaa aaaataacga
    11761 ttgaaaaatt atataaatct cttaaggaag agatttatga gataaattta aaacaaataa
    11821 taattgaaaa gggaaaaaaa tcacttgaga gaatgcttga gtatctttaa tttcccaggg
    11881 acacctacga ggtgtccgga gggtaaaaat ggaatatacg gaaaaagttc ttgaatattt
    11941 cagaaatcca agaaatatgg gtagaattga gaatgcagat ggaataggta aagttggcaa
    12001 tcctgtctgt ggagatgtta tgtggatata tattaaggtt gggaagaatg aaaaaggaga
    12061 agaaattatt gaagatataa aatttgaaac atttggatgt ggagcagcaa tagcaacaag
    12121 ttctgttgta acagaacttg caaaaggaaa aacaattgaa gaggcagaaa aaataacaaa
    12181 taaagaggtt gttgaatttc ttggcgggct tccaccggta aaaaaacatt gttcactttt
    12241 agcagaagaa ggacttaagg cagcaattga ggactataag agaaagaaaa atgaaagaaa
    12301 agggtaagat tatagaatca aaagaagata aagcacttgt tttgatggaa gagaaagaga
    12361 aatgtaaaag ttgtgggctg tgtaaaaaga ttattccaag acaaccagtt attgaagtag
    12421 aaaatattat aaaagcagaa gttggtgata atgttgagat tgaaataaaa gaggatgatt
    12481 tgtttgaagt ttctatttat atttatggtt ttccactttt gggctttata ctcggtatta
    12541 tttctgctta ttttttgaat aatatgtttt tgaaggttat aatttttctt ttgtttttga
    12601 tttcattctg ggtatttgga tttagaaaag gaaggattta tggagaaaga gcaaaaccaa
    12661 aaattatatc taaaatataa agatgatttt gaagaggcaa aaaaatactg gatggctttc
    12721 tggaaaggag aaataattga tagaccttta cttattttat cttttccaaa agatgaaaac
    12781 aaaaaagttt caggtccatc ttatttatca ggaatggatg gagattttga aaaacctata
    12841 agtcaatttg aagagttttg tgaaaataca atattttttg ctgaatcaat tccatcttta
    12901 aatatttctt ttggacctga tactgttgct tattgcgtaa aaggtgcttc tctcaatgtc
    12961 aggattaaaa gtcaaactgc atggattgaa ccatttgttg atgactggga taaatttaaa
    13021 aaaatagaaa tagaaaaaga tggtgaatgg tataagaaat ttatttgttt ttatagatat
    13081 gcttcagaaa gaggggacga caaattttta ttacaaatgc ctgactttca cacacatatt
    13141 gatttattga gggctataag aggaagtgaa aatttatgta ttgatttgat tgaaaggcct
    13201 gattttataa aaaaactcct tgatgaaata aaagacgttt ttattgacat ttatacagaa
    13261 atttacagtt caacagatat gaaaaaatgg ggaacaactt gctggatacc tttttattca
    13321 gaaaaaaaat actgtacaat tcaatgtgat tttatatgta tgatttctcc tgaaatgttc
    13381 agaaaatttg ctcttcctta tattgaatat gagacagatt ttcttgacca ttcaatattt
    13441 catcttgatg gaccgggtgc tttaaaacat cttgatgatt tactttcaat taaaaaatta
    13501 aatgctatac aatgggtaga aggagcaggt aataaacccc atatttactg gattgattta
    13561 ttcaaaaaaa taattaaagc aggaaaatca gttgtaatat atccacgctc ttttgaagaa
    13621 attaaggttt ttcataaaga acttggacca aaaaaaattg tctatcaacc agcatggcag
    13681 attaaaaatt taaaagaggc agaagagata aaaggttggt taataaaaaa cagttaagga
    13741 ggcaaaatgg atttaaaaga aaaggttgaa aattcaatta aggatgtaag agaattttta
    13801 caggcagaag gtggagattg cgaaattgtt gaagcaaagg atggaattgt taaagtaaga
    13861 ttgattggaa catgtaaggg ctgtcctttt tcacagataa ctttaaaaat gcggatagag
    13921 gcaataataa aagatagagt cccagaggtt aaagaagttg ttgaagttga ggaataatgg
    13981 aagaaaaact gaaaaaactt aaggattatc ttaaaaaatt aaagagggtt gttattgcat
    14041 attcaggcgg agttgacagt acttttttgc tcaatgtcgc cttaaacaca cttggtaaag
    14101 aaaatgttat tgcagttatt ggtatatctc ctacatatcc taatgaagaa agaaaatttg
    14161 ctgtaaattt ttgtaaggaa aatgggataa aatatattga agttgagact gaagaattta
    14221 aagattcaaa ttttttaaaa aatcctcctg atagatgctt ttggtgcaaa aaagagttat
    14281 ttggaaagat tgaagagata agaaaaaatt tgaattttaa atatataatt gatgggacta
    14341 acaaagatga tgagaaggac tacaggccag gagaaaaagc aaaaaaaatt tataatgtta
    14401 tttctccttt aaaagaatgc ggttttacaa aaaaagatat aagagaattg tcaaaagaat
    14461 taaatttacc aacatggaat aaacctcaga tggcatgtct tgcttcaagg ataccctatg
    14521 gagaaaaaat aacagaagag aaattgaaaa gaataaatga aggagaaaaa tttttatatt
    14581 ctcttggatt taaaattgtg aggataagag attacaatga actggcaaga attgaggtag
    14641 ataaaaatga aattgaaaaa ttgataaaat ttaaagaaaa aataattgaa aaattgaaaa
    14701 agattggtta caaatacata acagttgacc ttgaaggata tagaacaggc agtatgaatg
    14761 aggttttaag gtgaaagacc catttttatt ttactcaatt catttatttc tttttctatt
    14821 ttttccagcg gatattgaag attataaagt ttataataca atcccttttc ttttaataac
    14881 tgaagatggt ttcctgtttc aacaatctct ccttcattta aaacaaaaat tctatcaaca
    14941 ttccttacaa gagacaatct atgagcaatt ataaaaacag ttcttccttc aatcaatctc
    15001 tgcaatgctt ccattattaa actttcagat tcagcatcaa gtgaagatgt aaactcatca
    15061 aggattaata tggagggatt tttaagaaaa actcttgcaa ttgctatcct ttgtttctgt
    15121 cctacagata aagaatttcc catatctcca acatccgttt cataaccatc aggtaaattt
    15181 gaaataaaat catgggcatt tgcaagtttt gctgcttcta taacctcatt ttcacttaca
    15241 ttttttttcc caaaagtaat attttccatt actgttccac tgaaaataaa tggctcctgt
    15301 aaaactattc ctatttgaga ccttaaataa aaaattttgt aatcttttat atttttatca
    15361 tctatataaa ttgaaccttt atttacatca taaaatctcg gaattaaact tacaagagta
    15421 gattttcctg cgccacttgg tccaacaaga gcaatttttt ccccttcttt aacatcaaaa
    15481 gatatatttt tcagaacata cttttcatct tcatatgcaa aataaacatt ttcaaatttc
    15541 acctttccct tcaagtttgt agaagggaat atagaatttg gagagtcctt cacatctgaa
    15601 tatgcatcca ttatttcaaa aattctttct ccagcagccc ttgcatgctg catcatatgg
    15661 tttacctgat ttaattgatg aacaggtgtg taaagtaaat gaagataccc tatatatgca
    15721 acaagagttc caacacttat ttctcctgat accaccttta tacttccaaa ccagataatt
    15781 atcaaatatc ctaatgaact taaaaaaatt atccaactct gataagtagc ccataattta
    15841 gcaacataaa gatttatttt aaaaaacttc ctgcctttaa aagaaaattt tgcaagttga
    15901 tgtttttctc ttgcatatgc ttttatttct ttcattccga atatattttc ctgaagagaa
    15961 gatgtaagtt cccctctaat ttttctaact tctctatatc ctttatgtgc tttttctgta
    16021 aaaacatata caagaaaacc aattccacct attggaaaca tcatcaataa agtcaaaact
    16081 ttattcaatt taagtaagat aaaaaatgct ccaaaaaatg taaaaataga cataagaaaa
    16141 attatgaatc catcagttat
//
LOCUS       DAOSAT010000035        15492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2610_length_15492_cov_4.567986, whole genome shotgun sequence.
ACCESSION   DAOSAT010000035 DAOSAT010000000
VERSION     DAOSAT010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15492
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2610_length_15492_cov_4.567986"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..456
                     /locus_tag="PLF90_04285"
     CDS             100..456
                     /locus_tag="PLF90_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014488412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56531.1"
                     /translation="MIYHKNLAEGRWFEISFPEQMANIGMEVLRGIKWKKKDEKYLNL
                     AISRAIELIDLTKMDPKNKKRLKEICRLKEVLIDYFWGNNEYKSTDEKWENYFNSFIV
                     YAGKLRENKRRIVCQK"
     gene            444..2648
                     /gene="acsB"
                     /locus_tag="PLF90_04290"
     CDS             444..2648
                     /gene="acsB"
                     /locus_tag="PLF90_04290"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HOK56532.1"
                     /translation="MSKIIASAAINGAHKIVNIAEEKWKNAMDKYGPNQDVAFPDTAY
                     YLPIIYGILGIPISKLSHMEKVFKICRELLPEPVGKECHLPYLGPVLDAGMATLFAEE
                     IMEAIRYIEQPDFYFPDEEPPEDKLWLGAANDIILRKRGIEFVDGTAPGFAAVVGAVP
                     DNETAMKIATELIEKNLYVFMCAEYEGKRFAEQLKEGGMQLGWPVRLVPFGPDIYQAV
                     FALGFAVRAAMSFGGVEPGDYRRILIYNKDRIFAFVLAFGYVNEEWYANAAGAINFGF
                     PTIADTPIPQILPTGICTYEHVVSNIPHSEIVSKAVEVRGLKVSVSKVDIPVPYGPAF
                     EGERVRKEDLYVEIGGGKSIACEWTTSLPLDKVEDGKIEVIGPEIDDIPEGSTIPMAT
                     VIEVAGREMQEDFEPILERQIHDYINYINGVMHIGQRDIVWIRVSKKAKEKGLKFRHL
                     GVAIHAKYHEVFSNILDKVQVKIYTEEEKVKEIIEKARKVWRHRDERLAGITDEEVET
                     FYSCTLCQSFAPNHVCIVTPERTGLCGAFSWLDCRASNRINPTGPNQPVKKGKVIEEK
                     YGQWDGVNEFVKKASRGAIEKVSVYSLMVDPMTTCGCCECIAVVLPLCNGVMTVNREY
                     KGETPCGMKFTTLAGNVGGGRQIPGFLGHSKFNITQKKFLQAEGGILRLVWMPKMLKE
                     EIKERFVKRAEEVGIPDLLDRIADETIGITEEEILPFLQEKKHPALELPPIM"
     gene            complement(2774..3010)
                     /locus_tag="PLF90_04295"
     CDS             complement(2774..3010)
                     /locus_tag="PLF90_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56533.1"
                     /translation="MRCSETGYFIVDVGFMDWTGWKKFEFPKEKFEFVGSFPDWENIE
                     YIFIRANGDIAASKGEIVKEIEGSFYIEEVSIIQ"
     gene            complement(3007..3351)
                     /locus_tag="PLF90_04300"
     CDS             complement(3007..3351)
                     /locus_tag="PLF90_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56534.1"
                     /translation="MRNILKVFSILLFLFSVNLFSEEIKIFQGKEGKTDWDIVVLKNA
                     FSGSISICKDKEFTREENPSLKLTYKLSDKPEYNVVDFRKKNKNKDFSKMKSISIWIY
                     GNGDKKYISSWD"
     gene            complement(3348..>4611)
                     /locus_tag="PLF90_04305"
     CDS             complement(3348..>4611)
                     /locus_tag="PLF90_04305"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOK56535.1"
                     /translation="EMPASSPIHLFYKSVDRIKFTIVNREDNPHSGKFYISQMKFISP
                     VLIQGLTVRKPEKKQFLSFIFDTWGAGGNDEEIEKNVETIKNIGINLHIIPVSFPSDI
                     PEKNIENRKWLEGKIKSFMDKGIKIGISFYNTPPDEFAKKHPELLMKNENGEIYDTGG
                     FFLSPWHPEVEKLWVNYIIDTLNFLKERNLLEKIEVIMLCPGHESEISYEWSHIWAFD
                     ENAIASYRDYLKKKYRNNIIFLNEDMGTDYKSFADIKPPKNFYPDREHWVFVDFYRLS
                     MLKWCVKLADAVKEVFTPSYWLWLPHTHPDYPGRFFSGRYPIFYIENLKKLDIVDYVH
                     IPALDWQSIDDVNLIKKMGVKVIGEVDVVPSPARLEWTFNQSLKYGFDGVYIGIIENI
                     CRDGKLTEAGEICRKLIDVFKSKKGGEK"
     assembly_gap    4612..4621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4622..4984)
                     /locus_tag="PLF90_04310"
     CDS             complement(<4622..4984)
                     /locus_tag="PLF90_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56536.1"
                     /translation="MIVNSIAVNQKKLIERIPEYVQEKKVGIETFNISRWIFSEGVQI
                     KRARFLKENCLEIKYEFKDNQKKIDIDFMLPENHFEGERIGFKLYGNGKKDKIEVWCK
                     YRDSFIPLSMISLGQEKWQ"
     gene            complement(5153..5896)
                     /locus_tag="PLF90_04315"
     CDS             complement(5153..5896)
                     /locus_tag="PLF90_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOK56537.1"
                     /translation="MKNKFSKSIFSKPHGFTLIELLVVIAIIAILAAMLLPALSKARE
                     KARQAVCMNNLKQVGLALQMYLEDYNEYYPRPYLSVSPLQFISEAKDWNGGKYIKNPY
                     WNDPWGQYYPPITHCPSDTRKDHNFPMVTYAVNRAIYEWPTGTTFSLKYSRIKNPSNF
                     IVVTESFVPVTDALSWDYGFFERLKKYKDGGNLLSLHSDGLNFLFADGHVSWYEPYNA
                     GISSSSFVNFDWNTWNNSDYKKWWNPECR"
     gene            complement(6553..8367)
                     /gene="priA"
                     /locus_tag="PLF90_04320"
     CDS             complement(6553..8367)
                     /gene="priA"
                     /locus_tag="PLF90_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOK56538.1"
                     /translation="MKLVEVIFDIPLERSFYYLYEYEINNFIRVSAPLGEKKRKGFVI
                     NVNDLLEKRSEYKLIEKIYDINPLITEEIFEFSKIISEKYYVSSGQIIFSIIGNLPFK
                     YEKKFSEKKEKDSLLFTQKFKKEIFLFGDENEKLNFYIDLISHNRGSLFFLFPEVSIL
                     KDYYMKIKESTERKVIEYYGEMDKKERMNNYMEVLNSCDLIVLGTRISTFLPFKDLSL
                     IIVDSYIDASYREKKYPKYNAVEVAEERCKFQNIPLILTSHSLSICDYFEIKKKKVYL
                     VDKRNFEETPEIFILDKRWDEMDKNLDFLTKFSVSLIEETVLKGKKVGIIHNRKGTSK
                     TFKCSNCGHVLRCKKCNSILILNEENKLYCKYCKIFENFIKKCPHCKSREIIERMVGI
                     EKIYKELKKTYPDFKIQKFTAEEKLIEKGVDIFVGTHVIKNILDEFDFGLIIFPHADS
                     FLNIPEYNSEEIFFFILNDFIFRLRNKNTKIVIQTKNPNFEIFTSLKTKNYEVFYEKE
                     IKTREIVGYPPFSDIILIEIPVKRSPAFENKVNLLRKIIENSNLEIIFSDIVDDKRGK
                     KKVKMILKAQDDKRLDYKEVMDIREKLDFKIEINPPVF"
     gene            complement(8364..9254)
                     /locus_tag="PLF90_04325"
     CDS             complement(8364..9254)
                     /locus_tag="PLF90_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOK56539.1"
                     /translation="MDKIFENKKFVILDGAMGTYLEKLGFKGITPEIANIEESEIVKR
                     IHTEYCECGSEIILTNTFGANRKILEKKKLIDKFEKIIKDAFKIASSVKEKFKNVLIA
                     GDIGPTGELLTPYGNLEVNEAKNIFKEIGKNFEETAVDFLVLETFQDLEELKIAFDTL
                     RENTDFFIVPCLTFTYGREYRTLMGQKIEDYVKWAENNKVSIIGSNCGLSSGEMIEFV
                     KIIKELTGLTLWIKPNAGKPQISEGKIVYQENIEEFSKNCLNIVENRVKFIGGCCGTT
                     PLHIKNLKEILIKLFSNTNQ"
     gene            complement(9247..12168)
                     /locus_tag="PLF90_04330"
     CDS             complement(9247..12168)
                     /locus_tag="PLF90_04330"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOK56540.1"
                     /translation="MIEKRLIDGLKNPDVIYRSAPFWSWNDDLKIEQLKYQVDKMKEG
                     GFGGGFMHSRIGLITSYLSDEWMDCIKEVVKYAKENGMKMYLYDEDRWPSGFAGGIVA
                     KKKENRIKILKVWKKGKKWMKKVLEGVKSDWYNGLTYVDTMNEKTINDFIKSTYEKYV
                     KFLKNYIPNTVPAIFTDEPNYFHGRTVNKNDKNFYYFPWTKNLRKIFKSKFGYDIYER
                     INLLIEEKEDYEKVRYDYSRLISELFIENFGENIYNFCEKNRISLTGHYLGEDTIKTQ
                     ISVVGDVMSLYEFMQWPGVDHLGRNLKNPLTLKQCSSVSNQLGKERVLSELYGCSGQN
                     FTLAERKWIGDWHISLGINFFCPHLYLYSLKGCRKRDFPPTISHHQPYWGYQKELEDY
                     FARLNFIMSQGKFLANILVIHPVETGWSLKGSEKLTDYDEELSQLTQKLLENNFEYEF
                     GSEKLIEKYGEVKDGKFKIGRGEYEYIILPAMTTLREKTLKLIEEFINQGGKVFASEK
                     FFPYLIEGERKDISIKRSCILYSSIQSLIKELKNVVERDFEIYGDNNQEILDIFIHRR
                     KIDEDIEAIFLNNISLDKSYKVFINLKYEGKVYSLDLFNGKINVKGVKKEKKGILIED
                     YFPPVGSSLFLIDKSESPLILNENRKEEKILKSYIENWKIEPLSENVLVIDMCKFKKK
                     KDKRWSGKMPVLQIQQLFEKENVRQKIKLLFEFENENFSNDLYLVVEQLEKYNLKINN
                     KILKKDCMEEWLDPSFKKIKIPKNYIKRGKNKIEMDVTFIPPEFKNTLIFRKDGIEIE
                     NIYLLGNFVVKAKKVEYKNGGYYLNNFTIAVKEKEINEDDINLQGYPFYSGSIKAFKE
                     IEIKDIKKTFLKFEEFKCVVAKIKINEKQKGIVYLPPYEIEITEYLKEGKNIIEIELF
                     SSLRNLLGPHHLKEHNPEFVGPFSFVDWGKKWFKSKDRTVDYSVLPFGIGKICLIEKN
                     G"
     gene            complement(12146..13042)
                     /locus_tag="PLF90_04335"
     CDS             complement(12146..13042)
                     /locus_tag="PLF90_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOK56541.1"
                     /translation="MIKFVYSEEFLKYREISHPDSPERVESIVNYLQKKGNFRFVEPN
                     PCMEEDLYLVHTPEMVKKVKENDFFDPDCPNIPNIFYYACLSAGAAIRASEIALSGEI
                     GFSLGRPPGHHAGKNNIGGFCYFNNIAISVKKLLLENYKVAVLDIDGHHGNGTEEILK
                     NEKDVIFVSIHQYPAYPGTGKYSFENCYNFPIPPYTSSKKYMEKFDRCLEIIKDFSPD
                     IIGVSCGFDAFKEDPLLQLSLTETDYYIIGKKIASLGKKIFLILEGGYNIFKIGNLVY
                     CFVSGFEKVLKMEASNDRKKID"
     gene            complement(13039..13797)
                     /locus_tag="PLF90_04340"
     CDS             complement(13039..13797)
                     /locus_tag="PLF90_04340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOK56542.1"
                     /translation="MEKIRSGYVLKGYILKEMVGSGGFSTVYRAESIEPLPLYNSIIA
                     VKVLHPRRFERNQIKQFIKEGKIAKSLEHPNIVKVFDVVQENGNFFILMEYLDTDLIK
                     AIRTKKYLFNEKNVIDIIIKAAKGIAYIHQNGIVHKDINPSNILITYSLEKIKITDFG
                     LSKVDRGILNRGEFRGGTEGYVAPEIYKGKKADKKSDIYSFGKTIEKIYRELNFPFPE
                     KIKNIVKIATEPEPENRFESMEGIIYILETRKIE"
     gene            13906..14535
                     /locus_tag="PLF90_04345"
     CDS             13906..14535
                     /locus_tag="PLF90_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOK56543.1"
                     /translation="MRIEDVREIKKVKIFIDNEKIDGEIIDIDKWGKMIISVPHRTKT
                     IPVELGKRGEIFFEKNGLEFFISGKIFSQGIERMLFLPETDILTEKRKKERFETPFIK
                     CILTKIPYKFHKEVIIGNIIDISFSGTKVETPIALNINFFYELKTDFIINRKSYSFSV
                     KCKPKYINKKRNIFFCGLEFTEMDYFSLENLKKYIKELAHQLKRDALNY"
     gene            complement(14538..>15492)
                     /locus_tag="PLF90_04350"
     CDS             complement(14538..>15492)
                     /locus_tag="PLF90_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOK56544.1"
                     /translation="RIPQDGYFKMKLYGRDVDLRVSTFPTIFGEMVALRVLDKKNIIS
                     GLEQLGFFPEVLHRWRILLDEPYGMILVTGPTGSGKTTTLYSSLNELDSTHRKIITVE
                     DPVEYHLKNVNQTQINPKSGLTFSIALRSILRQDPDIIMVGEIRDIETAEIAFRAAQT
                     GHLVLSTLHTNTAPGAIIRLLDMGVESYLISSSLIGVLNQRLVRSICVSCKEEYKPYP
                     EEIKMLDPSLLEKDNLKFYRGKGCHLCNGTGYGGRTGIFELMVINEELRRVIIKNPDM
                     IEIKEIAKKSGMESLREDGIKKVLAGITTISEVLYTTRKED"
BASE COUNT         5293 a         2502 c         1958 g         5729 t
ORIGIN      
        1 taataaatat aagaccttct tttggaaaca aatcaagaga agtggaaaat ccagaggtta
       61 gaaaaaaaat tgttgaaatt gtaaaggagt taatagaata tgatttatca taaaaatctt
      121 gcagaaggta gatggtttga aatttctttt cctgaacaga tggcaaatat tggaatggag
      181 gttttaagag gtataaaatg gaaaaaaaaa gatgaaaagt atttaaatct tgcaatttca
      241 agagctattg aacttattga cttaacaaaa atggacccaa aaaacaaaaa aaggttaaag
      301 gaaatttgca gattaaaaga ggtcttaatt gattatttct ggggcaataa tgaatataaa
      361 tccactgatg aaaaatggga aaattatttt aattctttta ttgtttatgc aggtaaatta
      421 agagaaaata aaaggaggat tgtatgtcaa aaataattgc ttctgctgct ataaatggag
      481 cacataaaat tgtaaatatt gctgaagaaa aatggaaaaa tgcaatggat aaatatggtc
      541 caaatcaaga tgtcgctttt ccagatactg cttattattt accaataatt tatggaattc
      601 ttggaatccc tatttcaaaa ctttcacata tggaaaaggt ttttaaaatt tgtagagaat
      661 tattacctga acctgttgga aaagaatgtc atctccctta tcttgggcct gttcttgatg
      721 ctggaatggc aactttattt gctgaagaga taatggaagc gataagatat atagaacagc
      781 ctgattttta tttccctgat gaagaacctc ctgaagataa actctggctt ggtgctgcaa
      841 atgatataat tttgaggaaa aggggtattg aatttgttga tggtaccgct cctggttttg
      901 ctgctgttgt tggagcggtt cctgataatg aaacagcaat gaaaatagca acagaactta
      961 tagagaaaaa tttatatgtt tttatgtgtg ctgaatatga aggaaaaaga tttgcagaac
     1021 aattaaaaga aggtggtatg caacttggct ggcctgttcg tcttgttcca tttggtcctg
     1081 atatttacca ggcagtattt gctcttggat ttgctgtaag ggctgctatg agttttggag
     1141 gagttgaacc aggtgattac agaagaattt taatttataa taaagataga atatttgcat
     1201 ttgttcttgc ttttggatat gtaaatgaag aatggtatgc aaatgcagca ggagcaataa
     1261 attttggttt cccaacaata gcagatactc caatccctca gattttacca actggaatat
     1321 gcacttatga gcatgttgtt tcaaatattc cacattcaga aattgtttca aaagcagttg
     1381 aggtaagagg attaaaggtt tctgtctcaa aagtagatat tcctgttcct tatggacctg
     1441 cttttgaggg agaaagagta agaaaagagg acttatatgt tgagattggt ggtggaaaga
     1501 gtattgcatg tgaatggaca acaagtttac cattggataa agttgaggat ggaaaaatag
     1561 aagttatagg acctgaaatt gatgatatac ctgaaggaag tacaataccg atggctactg
     1621 ttattgaagt cgctggaaga gaaatgcagg aagattttga accaattctt gaaagacaga
     1681 ttcatgatta tataaactat ataaatggag taatgcatat aggacaaagg gatattgtat
     1741 ggataagggt aagtaaaaaa gcaaaagaaa agggattaaa atttagacat cttggagttg
     1801 caattcatgc aaaatatcat gaggtatttt caaacattct tgataaagtt caggttaaaa
     1861 tttatacgga agaagagaaa gtaaaagaaa taattgaaaa agcaaggaaa gtatggaggc
     1921 acagagatga gcgacttgca ggaattacag atgaggaagt tgagacattt tattcatgta
     1981 cactatgcca gtcatttgca ccaaatcatg tatgtatagt aactcctgaa agaacaggac
     2041 tttgtggtgc attctcatgg cttgactgta gggcttcaaa tagaataaat cctactggtc
     2101 caaatcagcc agttaaaaaa ggaaaagtta ttgaagaaaa atatggtcaa tgggatggag
     2161 ttaatgaatt tgtaaaaaaa gcaagcagag gagcaattga aaaagtcagt gtttacagtt
     2221 taatggttga ccctatgact acatgtggat gctgtgaatg tattgcagtt gttttgcctt
     2281 tatgtaatgg agtaatgaca gttaacagag aatataaagg agaaacacca tgcggaatga
     2341 aatttacaac actggcagga aatgtaggtg gaggaagaca gataccgggt tttctcggtc
     2401 attcaaaatt caacataact cagaaaaaat ttttgcaggc agagggtgga attttgcgac
     2461 ttgtatggat gcctaagatg ttaaaagagg aaattaagga aagatttgta aaaagagcag
     2521 aagaagttgg aatacctgat ttacttgata ggattgcaga tgaaacaata gggataacag
     2581 aagaggaaat tttaccattc ttacaggaga aaaaacatcc agcccttgaa cttcctccaa
     2641 ttatgtaagg tcggaggacg gggttatata gaaatgcaaa aacaaaaatc ctgataggag
     2701 aacttaaaac atattactta aaaattgaaa agttacgaat taattttttt gatgaaaaaa
     2761 atccattttt gttttattga attatagaaa cttcttctat ataaaaactt ccctctattt
     2821 ccttaactat ttcaccttta cttgcggcta tatcaccatt tgctcttatg aatatatatt
     2881 caatattctc ccagtcagga aaagaaccta caaattcaaa tttttctttt ggaaattcaa
     2941 actttttcca accagtccag tccataaaac caacatccac aataaaataa cctgtttctg
     3001 agcatctcaa tcccagctgg agatatactt tttatcacca ttaccataaa tccagattga
     3061 tatacttttc atttttgaaa aatccttatt tttatttttc tttctaaaat ccacaacatt
     3121 atattctggt ttatcactca atttgtaagt taattttaaa gacggatttt cctctcgtgt
     3181 aaattcttta tccttacata tagaaattga accagagaat gcatttttta gtacaacaat
     3241 atcccaatct gtttttcctt ctttcccctg aaaaattttt atttcttccg aaaataaatt
     3301 tacagagaaa agaaaaagta aaatagaaaa aacttttaaa atatttctca tttttcacct
     3361 ccttttttac ttttaaatac atctattagt tttctacaaa tttctcctgc ttctgtcaat
     3421 tttccatctc tgcagatatt ttcaattatg cctatgtaaa caccatcaaa accgtatttt
     3481 aaactctgat taaatgtcca ttcaagcctt gctggacttg gaacaacatc tacttctcct
     3541 attaccttaa cacccatttt ttttataaga ttcacatcat caatagattg ccagtccaga
     3601 gcaggtatgt ggacatagtc aacaatatca agttttttca aattttctat ataaaaaata
     3661 ggatatcttc ctgaaaagaa acgacctgga taatcaggat gagtatgggg taaccaaagc
     3721 cagtaagaag gtgtaaaaac ttctttaaca gcatccgcaa gtttaacaca ccatttaagc
     3781 atacttaatc tataaaaatc aacaaaaacc cagtgttctc tatcaggata aaaatttttc
     3841 ggtggtttta tatctgcaaa agatttataa tcagtgccca tatcttcatt aagaaaaatt
     3901 atattgtttc tatatttctt ttttaagtaa tctctataac ttgcaattgc attttcatca
     3961 aatgcccaaa tatgagacca ttcataagaa atttctgatt catgtcctgg acataacatt
     4021 attacttcga tcttttccag caaatttctc tcttttaaaa aatttaaagt atcaattata
     4081 taattcaccc ataatttttc tacttcagga tgccaaggag aaagaaaaaa accgcctgta
     4141 tcataaattt ctccattttc gtttttcatt aaaagttcgg gatgcttttt ggcaaactca
     4201 tcaggtggtg tattataaaa acttatgcca atcttaatcc ctttatccat aaatgattta
     4261 atttttcctt ccagccattt tctgttttct atgttttttt caggtatatc agaaggaaaa
     4321 ctaactggga ttatatgaag atttattcct atatttttta ttgtctctac atttttttca
     4381 atctcttcat catttccacc tgcaccccat gtatcaaata taaaagataa aaactgtttt
     4441 ttttctggtt ttcttactgt tagcccttga ataagaacag gactaataaa tttcatctgt
     4501 gaaatataaa atttaccaga atgaggatta tcctctctat ttacaattgt aaattttatc
     4561 ctatctacac ttttataaaa aaggtgtatt ggactacttg caggcatttc tnnnnnnnnn
     4621 nttgccattt ttcttgtccc agggaaatca ttgaaagagg aataaatgaa tctctatatt
     4681 tacaccatac ctctatttta tctttttttc catttccata aagtttaaat cctattcttt
     4741 ctccttcaaa atgattttca ggaagcataa aatcaatatc tattttcttt tgattatctt
     4801 taaattcgta ttttatttcc aaacaatttt cttttaaaaa tcttgctctt tttatttgta
     4861 caccttcact aaatatccat cttgagatat taaatgtttc tattccaacc tttttctctt
     4921 gcacatattc aggaattctt tcaattaact tcttttgatt tacggctata gaatttacta
     4981 tcattaaaaa aaatatcgga atattaaaat gtcttttaca actggtcaaa aaaattttta
     5041 aatttaacgt cctactcctc tcataaagtt tttctaaata aaactttaaa aagtttttca
     5101 ttgtaaaaca aaaattcaat ttttaacatc acaccaaata aatttgactg cattatctac
     5161 attccggatt ccaccatttt ttataatcag aattattcca cgtattccaa tcaaaattta
     5221 caaaactaga acttgaaatt ccagcattat atggttcata ccatgaaaca tgtccatctg
     5281 cgaaaaggaa atttaaacca tcagaatgca aacttaaaag attcccacca tctttatatt
     5341 tctttaatct ctcaaaaaaa ccataatccc aagataaagc atctgttaca ggaacaaaac
     5401 tttcagtaac aacaataaaa ttactcggat ttttaatcct tgaatatttt aatgaaaaag
     5461 tagttcctgt aggccattca tatattgctc tatttacagc gtatgttacc attggaaaat
     5521 tatggtcctt ccttgtatca cttgggcaat gagttatagg aggataatat tgcccccatg
     5581 ggtcattcca gtaaggattt tttatatatt ttccaccatt ccagtccttt gcctcagaaa
     5641 taaattgtag aggacttaca ctaagatatg gacgtggata atactcatta taatcctcta
     5701 aatacatttg taaagccagt cccacctgtt taagattatt catacatact gcctgtcttg
     5761 ctttttctct tgccttactt aatgctggta ataacatcgc tgcaagtatc gctattatcg
     5821 caattactac taaaagttct atcagggtga aaccgtgtgg ttttgagaaa atgctctttg
     5881 aaaatttgtt cttcataatt cacctccttc taattttgct aaatttctgt atttttcaac
     5941 tattataact tgttttagta aattctactt aaatataata taatttttta attttcttgt
     6001 caaatccaaa aaactccttt caggttaaga catagtatcc atggatataa tatacaagaa
     6061 agaaatattt ggctaagaag acaatagaaa tattagttct caagagtaaa aaagcaaggg
     6121 ggaagcccac tcaataagtt agaaatatta tacccaggtt ataaagaatt attaaatgta
     6181 atatattttt tgatgcgtaa gtaaagaaga attttttgag attattaaga tggcagtaga
     6241 tatataacag aaaaagagta tattcaacga tagggatgac accttatgag aaattttgta
     6301 aatatagtaa attaccttct gaagaagttt gtctattctc tgttattaat ctagatgaaa
     6361 taactgatat tttaaactct aatttaaaat ttttctctta ctcctgaata aacctttttt
     6421 aaccaagggc cacaaagttc ctcaaaaaat ctctataaaa caaattgctc ccttaaagat
     6481 aaaatttaca gaaataattt actttttgtg tcttcgttta acttcgcatt gtactatatt
     6541 ggatgactgg gctcagaaga caggaggatt gatttctatt ttaaagtcaa gtttttcacg
     6601 aatgtccatt acttctttat aatccagtct tttatcatcc tgtgccttta aaatcatctt
     6661 tacttttttc ttgcctcttt tatcatctac aatgtctgaa aaaattattt caagattgct
     6721 gttttcaata atttttctta aaaggttaac cttattttca aatgcgggac ttctttttac
     6781 cggtatttca ataagtatta tatctgaaaa tggaggatag ccaacaattt ctcttgtttt
     6841 aatttctttt tcgtaaaaaa cttcataatt ttttgttttt aaagaagtaa atatttcaaa
     6901 atttggattt tttgtctgaa ttactatttt tgtgttttta tttctcaatc taaatataaa
     6961 gtcattcaat ataaaaaaga aaatctcctc tgaattgtac tctggaatat ttaaaaaaga
     7021 atctgcatgt ggaaaaataa ttaaaccaaa atcaaattca tcaagaatat tttttataac
     7081 atgcgtacca acaaaaatat caactccttt ctcaattaat ttttcttctg ctgtaaattt
     7141 ctgaattttg aaatctggat atgtcttttt aagttcttta taaatctttt caattccaac
     7201 catcctttct attatttctc tgcttttaca atgaggacat ttttttataa agttttcaaa
     7261 aatcttacag tatttacagt acaatttatt ttcttcattt agaataagta ttgaattgca
     7321 tttcttacat cttaaaacat gaccacaatt tgaacattta aatgtttttg aagttccttt
     7381 tctattatga ataatcccaa ctttttttcc ttttaaaact gtctcttcta taagagaaac
     7441 tgaaaatttt gtgagaaaat ctaaattttt atccatttca tcccatcttt tatcaagaat
     7501 aaagatttca ggagtttctt caaaattcct tttatcaacc aaatatactt tcttcttttt
     7561 aatttcaaaa taatcacaga tagaaagaga atgagaagta agaattagag gaatattttg
     7621 aaatttacat ctttcctctg ctacttcaac agcattatat ttaggatatt ttttttctct
     7681 gtatgaagca tctatgtaag aatcaacaat tataagagaa aggtctttaa agggtagaaa
     7741 agttgaaatt cttgttccca aaactattaa atcacatgaa tttaaaactt ccatgtaatt
     7801 attcatcctc tcctttttat ccatttctcc ataatattct ataaccttcc tttctgtact
     7861 ttcttttatt ttcatataat aatctttcag gattgatact tctggaaata aaaaaaacaa
     7921 agaacctcta ttgtgtgaaa ttaaatcaat ataaaaattt aatttctcat tttcatcacc
     7981 aaaaagaaaa atctcctttt taaatttttg agtaaatagt aatgaatctt tttctttttt
     8041 ttctgaaaat tttttttcat atttaaacgg tagatttcca atgattgaaa aaataatctg
     8101 gccagaagaa acataatatt tttctgaaat aatctttgaa aattcaaaaa tttcttctgt
     8161 tattaaagga tttatatcat aaattttttc tattaattta tactctgacc ttttttctaa
     8221 taagtcattt acatttatta caaaaccctt tctttttttt tctccaagag gagcagatac
     8281 acgaataaaa ttatttattt catattcata cagataataa aaacttctct caagaggaat
     8341 atcaaaaata acttcaacaa gtttcattga ttagtgttag aaaaaagttt tattaaaatt
     8401 tctttaagat tttttatatg taaaggtgta gttccacagc atccacctat aaatttcact
     8461 ctattttcta caatattcaa acaatttttt gaaaactcct caatattttc ctgatataca
     8521 attttacctt cagaaatctg tggttttccc gcattcggtt ttatccacaa agtaagaccg
     8581 gtcaattctt taattatttt tacgaactca atcatttcac ctgaacttaa gccacaattt
     8641 gaaccaataa ttgaaacttt attattttca gcccatttca cataatcttc tattttttga
     8701 cccattaaag ttctgtattc tcttccatat gtaaaagtaa gacatggaac aatgaaaaaa
     8761 tctgtattct ctcttaatgt atcaaatgca atcttaagtt cttcaagatc ctgaaatgtt
     8821 tcaaggacta aaaaatctac tgctgtttcc tcaaaatttt tacctatttc tttaaaaata
     8881 ttttttgctt cattaacttc taagtttcca taaggtgtaa gtaattctcc agtaggtcca
     8941 atatctccag caattaaaac atttttaaat ttttctttaa cactggaagc aattttaaag
     9001 gcatctttaa taattttttc aaatttatct ataagtttct ttttttcaag tatttttcta
     9061 tttgctccaa aagtattggt taaaattatt tctgaaccac attcacaata ttctgtatgg
     9121 attcttttta caatttcaga ttcttcaata tttgcaattt caggtgtgat tcctttaaaa
     9181 ccaagttttt caagatatgt tcccattgca ccatccaaaa ttacaaactt tttattttca
     9241 aatattttat ccatttttct ctattaaaca gatttttcca ataccaaaag gtaaaacaga
     9301 atagtcaact gttctatcct tgcttttaaa ccatttctta ccccaatcaa caaatgaaaa
     9361 aggaccaaca aattctggat tatgttcctt taaatgatga ggtcccagta aatttcttaa
     9421 acttgaaaaa agttcaatct caataatatt tttaccttcc tttaaatatt cagttatttc
     9481 aatttcataa ggaggaagat aaacaatacc tttttgtttt tcatttattt ttatttttgc
     9541 cacaacacat ttaaattctt caaattttaa aaatgttttt tttatatctt taatttcaat
     9601 ttctttaaat gcctttatac ttccagagta gaaaggatat ccctgtaaat taatgtcatc
     9661 ttcattgatt tctttttcct ttactgcaat agtaaagtta ttcagataat aacctccatt
     9721 tttatattca accttttttg cctttacaac aaaatttcca agcagatata tattttcaat
     9781 ttcaatacca tcttttctga aaattaaagt atttttaaat tcaggaggaa taaaggtaac
     9841 atccatttca attttgtttt tacccctttt tatataattt ttaggtattt ttatcttttt
     9901 aaatgatggg tcaagccatt cttccataca gtcctttttc agaattttat tatttatttt
     9961 taaattatat ttttccaact gttcaacgac taaatataaa tcattactga aattttcgtt
    10021 ttcaaattca aaaagtaatt ttatcttctg tctgacattc tctttttcaa acaattgctg
    10081 aatctgtaaa acaggcattt ttccagacca tcttttatcc tttttctttt taaacttaca
    10141 catatcaata acaaggacat tctcagaaag tggttcaatt ttccagtttt caatataaga
    10201 ttttaagatt ttttcttctt tcctgttttc atttagaatt aaaggacttt cactcttatc
    10261 aatcaaaaaa agagaagaac cgacaggtgg aaaataatct tcaattaaaa tacctttttt
    10321 ctcttttttc acacctttaa catttatttt tccattaaac aaatccaaac tgtaaacttt
    10381 tccctcatat ttcaaattta taaaaacctt atatgattta tcaagtgaaa tattattcaa
    10441 aaaaattgct tcaatatctt catcaatttt tcttctgtga ataaaaatat ctaaaatttc
    10501 ctgattatta tctccataaa tttcaaaatc tctttcaact acatttttta attctttaat
    10561 cagggattga atagaagaat ataaaataca acttctctta atactaatat cttttctctc
    10621 tccttctatt aaatatggaa agaatttttc tgaagcaaaa actttcccac cctgattaat
    10681 aaactcttca atcagtttta atgttttttc ccttaaagtt gtcattgccg gtaaaattat
    10741 atattcatat tcacctcttc ctattttaaa tttcccatcc ttaacctctc catacttttc
    10801 aatcaatttt tcacttccaa attcatattc aaagttgttt tcaagaagtt tctgtgtcaa
    10861 ttgagaaagt tcttcatcat aatctgtaag tttttcactt ccttttaaac tccagccagt
    10921 ttcaactgga tgaattacaa gaatatttgc aagaaatttt ccctgactca taataaagtt
    10981 gagccgagca aaataatctt caagttcttt ctgatatccc cagtatggtt gatgatgaga
    11041 aattgttggc ggaaaatctc tttttctgca tccttttaaa gaatacaaat acagatgtgg
    11101 acagaaaaaa tttattccaa gagaaatatg ccagtcacca atccactttc tttctgcaag
    11161 agtaaaattc tgtcctgaac atccatataa ttcacttaat actctttctt ttccaagttg
    11221 attggataca gaactacatt gttttaaagt taaaggattt tttaaatttc tgccaagatg
    11281 gtcaacacca ggccattgca tgaattcata taaagacata acatctccaa caactgaaat
    11341 ctgtgtttta atggtatctt ctccaagata atgacctgta agtgaaatcc tatttttttc
    11401 acagaaatta taaatatttt ctccaaaatt ttctataaat aattcactta ttaaccttga
    11461 ataatcatat cttacttttt cataatcttc cttttcttca attaacaaat ttattctttc
    11521 atagatatca taaccaaatt ttgatttaaa aatttttctt aaattttttg tccatgggaa
    11581 gtaataaaaa tttttatcat tcttatttac agttcttccg tgaaaataat ttggttcatc
    11641 tgtaaaaatt gctgggacag tattaggaat atagtttttt aaaaatttaa catatttttc
    11701 ataggttgat tttataaaat catttattgt tttttcattc attgtatcaa catatgtcaa
    11761 accattatac cagtcagatt ttacaccttc taaaaccttt ttcatccact ttttaccctt
    11821 tttccagacc tttaaaatct taattctatt ttcctttttc tttgcaacaa taccacctgc
    11881 aaaaccactt ggccatctat cttcatcgta taaatacatt ttcattccat tttctttagc
    11941 atatttaacc acctctttta tacaatccat ccattcatct gataaataag aagtaataag
    12001 tcctattctt gaatgcataa aaccccctcc aaaaccaccc tctttcattt tatcaacctg
    12061 atatttcaac tgttctattt tcaaatcatc attccacgac cagaaaggag cacttctata
    12121 aattacatca ggatttttaa gtccatcaat caatcttttt tctatcattg cttgcctcca
    12181 tttttaatac tttttcaaat ccagaaacaa aacaataaac aagattacct attttaaata
    12241 tattataacc accttctaaa atcaaaaata tttttttgcc gagagaagca atttttttac
    12301 caataatata ataatctgtt tcagttaaag aaagttgtaa taaagggtct tctttaaaag
    12361 catcaaaacc acatgaaaca cctattatat caggtgaaaa atcttttatt atttcaagac
    12421 atctgtcaaa cttttccata tatttttttg aacttgtata aggtggaatt gggaaattat
    12481 aacaattttc aaaagaatat ttacctgttc caggataagc aggatattga tgaattgaaa
    12541 caaatataac atctttttca ttttttaaaa tttcttctgt tccattacca tgatgtccat
    12601 ctatatcaag aactgcaact ttataatttt ctaaaaggag tttctttaca gaaattgcta
    12661 tattattgaa ataacaaaaa ccgcctatat tatttttacc tgcatgatgt cccggtggcc
    12721 ttccaagaga aaaaccaatt tcacctgaaa gtgctatttc acttgctctt attgctgctc
    12781 ctgcggataa acaggcataa taaaatatat tcggtatatt tggacaatca gggtcaaaaa
    12841 aatcgttttc ttttactttt tttaccattt caggagtatg aacaagatat aaatcttctt
    12901 ccatacaggg attgggttct acaaatctaa aattaccctt tttttgtaaa taattaacta
    12961 tactttctac tctttcaggt gaatcaggat gggaaatttc tctgtatttt aagaattctt
    13021 cagaataaac aaacttaatc attcaatttt cctggtctca agtatataaa taatcccttc
    13081 catactttca aatctatttt caggttcagg ctctgttgct attttaacta tattttttat
    13141 tttttctgga aaaggaaaat tcaattctct ataaattttt tcaattgtct ttccaaaaga
    13201 ataaatatca ctttttttat ctgctttttt tcctttataa atctcaggtg ctacatatcc
    13261 ttctgttcct cccctaaatt cccctctatt taaaattccc ctatcaactt ttgaaagccc
    13321 gaagtccgta attttaattt tttcaagtga ataagttatt agaatatttg aaggatttat
    13381 atctttatga actattccat tttgatgtat ataagcaatc ccttttgctg cttttataat
    13441 tatatcaatt acatttttct cgttaaaaag atattttttt gttctgatgg cttttatcaa
    13501 atcagtatca agatattcca ttaaaataaa aaaattccca ttttcctgaa caacatcaaa
    13561 aaccttaact atattaggat gttcaagtga ttttgctatt tttccctctt ttataaactg
    13621 tttaatttgg tttctttcaa atcgtctcgg atgtaaaacc ttaacagcaa taattgaatt
    13681 gtataaaggc aagggctcaa ttgattcagc acgataaact gttgaaaatc cgccagaacc
    13741 aaccatttct ttcaatatat atccttttaa aacataacca gacctaattt tttccatttt
    13801 ttttctccta aacatttcta ttataaacca tttttatttt tttttcatca aatattgcta
    13861 ttttaaaaat gtcaagggta aaatagaatt aaaggggaaa tattaatgag aattgaggat
    13921 gtaagggaaa ttaaaaaagt taaaattttt atagataatg aaaaaataga tggagaaata
    13981 attgatatag ataaatgggg aaaaatgata atttctgtcc ctcatagaac aaaaacaatt
    14041 cctgtagaac tgggtaaaag aggagaaatt ttttttgaga aaaacggttt ggaatttttt
    14101 atttcgggga aaattttttc tcagggtatt gaaagaatgt tatttttacc tgaaacagat
    14161 attctaacag aaaaaagaaa aaaagagaga tttgaaacac cttttattaa atgtattcta
    14221 accaaaatcc cttataaatt tcataaagaa gtaatcatag gaaatattat agatatttct
    14281 ttttcaggta ctaaagttga aacccctatt gccttaaata taaatttttt ctatgaatta
    14341 aaaactgatt ttataataaa ccgtaaaagt tattctttca gtgtaaaatg taaaccgaaa
    14401 tacataaata aaaagagaaa catttttttc tgtggattgg aatttacaga aatggattat
    14461 ttttcacttg aaaatcttaa aaaatatatt aaagaacttg ctcatcaact caaaagagat
    14521 gctctaaatt actgactcta atcctctttt cttgttgtat aaagaacttc tgaaattgtt
    14581 gttattcctg caagaacttt ttttatcccg tcttctcgca gtgactccat accggacttt
    14641 ttagcaattt cttttatttc tatcatatca ggatttttta ttatcactct tcttaactct
    14701 tcatttataa ccatcaattc aaaaatacct gttcttccac cataaccggt tccattacat
    14761 aaatgacaac cctttcctct ataaaatttc aaattatctt tttcaagtaa tgatgggtca
    14821 agcattttta tttcttcagg gtatggttta tattcttcct tgcatgaaac gcatatactc
    14881 cttactaatc tttgatttaa tactcctatt aaagaagaag aaatcaaata tgattcaact
    14941 cccatatcca gaagtcggat aattgcacct ggggctgtat ttgtatgtaa agtggacaaa
    15001 acaagatggc ctgtctgtgc tgctctaaaa gcaatttctg ctgtttctat atctcttatt
    15061 tcaccaacca ttattatatc cgggtcctgt cttaagattg acctcaatgc aattgaaaaa
    15121 gttaaacctg attttggatt tatctgtgtt tgattaacat ttttaagatg gtattccaca
    15181 gggtcttcta ctgttataat ttttctgtga gtactatcaa gttcatttaa tgaagaatat
    15241 aaagttgttg ttttaccact acctgttgga cctgtaacca gaatcatacc gtaaggttca
    15301 tcgagaagga ttctccacct gtgaagaact tcgggaaaaa aaccaagttg ttccagaccc
    15361 gaaattatgt tttttttatc cagaactctt aaagcaacca tttctccaaa aattgttgga
    15421 aaggttgaaa ctcttaaatc aacatctctt ccatataatt tcattttaaa ataaccatcc
    15481 tgaggaatcc tt
//
LOCUS       DAOSAT010000036        15480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2614_length_15480_cov_4.994814, whole genome shotgun sequence.
ACCESSION   DAOSAT010000036 DAOSAT010000000
VERSION     DAOSAT010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2614_length_15480_cov_4.994814"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PLF90_04355"
     CDS             complement(<1..637)
                     /locus_tag="PLF90_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK56545.1"
                     /translation="MSIIIPARNEEENIKETIESIIPNINIEETEIIVVNDHSEDRTE
                     NIVSQLCKKYNFLKIVRNEKEPGFANTLKTGFENAKGEFVLPVMADLCDQPETIKKMV
                     KKAKEGFDLICGSRYSKKGKKIGGPKIQSLFSRFVGVSLYYLIGIPTKDCPNAFKMYR
                     RTILNSLNLKSKGFSISMEACLKFFFSGYKICEVPTIWYGRKKGKSKFKLSK"
     gene            complement(655..1767)
                     /locus_tag="PLF90_04360"
     CDS             complement(655..1767)
                     /locus_tag="PLF90_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006054814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOK56546.1"
                     /translation="MKKVLVTGGAGFIGSHLVDELVKKGYEVTVYDSLDPQVHPDGIP
                     DYINLNARYIFEDIRKKQELKKIIKEIDYIFHFAAKVGVGQSQYQIEKYVDVNIKGTA
                     NLLDILVNTKNNVKKLIVASSMSIYGEGLYKCEKCGNVKPNLRDFKDEKINDWEPKCP
                     YCKEKITPLPTPETLPLISNSIYAISKKEQEEMSLLIGKTYGIPVVSLRFFNVYGPRQ
                     TLSNPYTGVCAIFLSRIKNNNPPVIYEDGLQTRDFIWVGDVVNACILSMEKNEANYQI
                     FNVGSGKPVSILEMAELLIKLLGKDLKPEITYKFRKGDVRHCYADISKIKNKIGFEPQ
                     TTLEEGMKKLIEWAKETEAEDKFEKAQEELRKKGLV"
     gene            1843..2247
                     /locus_tag="PLF90_04365"
     CDS             1843..2247
                     /locus_tag="PLF90_04365"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOK56547.1"
                     /translation="MAKEINFLKERSKEFWNEGVRLYKEGKYNLSVFNIEQAIQLWIK
                     YLIGIKITDWPKTHYFSELIKSLSDVYEKKGIIEYYKKNEIFFNNLEDAYFTTRYFPK
                     NFSKNIANQFIENAKKFILFTENLTGEKFFNE"
     gene            2240..2596
                     /locus_tag="PLF90_04370"
     CDS             2240..2596
                     /locus_tag="PLF90_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOK56548.1"
                     /translation="MSKSLVDILKKRAEEKEKYFKNYLFYCKKIKEEAEKILGKVEVI
                     VFGSVVKNEYTPLSDIDVLIISDNLPNSYEERIKIKTEIKSKIDSFSPFQIHLSNYKE
                     YNNWYKKFIKNDFMKI"
     gene            complement(2583..3533)
                     /locus_tag="PLF90_04375"
     CDS             complement(2583..3533)
                     /locus_tag="PLF90_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOK56549.1"
                     /translation="MNKKILISGGAGFVGSHLCDFFVNNGYTVWCIDNFITSTKTNIK
                     HLLNNKNFVFIEKDLIYLNEDEIKEKFDYVLHFASPASPVDYMKYPIETLKVNSIGTE
                     KMLNIALKSKSTFLFSSTSEVYGDPLIPVQNEEYWGNVNPIGPRSVYDEGKRYGEAIV
                     MAYHRKYKLNTKIIRIFNTYGPRMRLNDGRVIPNFIYNALNNKPLPIYGDGNQTRSFC
                     YIDDLIRGIIKVLEVDYNLPINLGNPEEFTISQLAEKIEKIFGKKLGFEFLPPLPDDP
                     RKRKPDITLAKKLLNWEPQIKLEEGLKKTIEYYIRLSKSS"
     gene            complement(3526..4857)
                     /locus_tag="PLF90_04380"
     CDS             complement(3526..4857)
                     /locus_tag="PLF90_04380"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOK56550.1"
                     /translation="MRNIGIIGAGHVGLTTGACFSKLGNKVICCDNDVEKVKKLKNLI
                     MPFYEPGLEEIVKEGVEKNLLTFTDSIYETVENSEIIFIAVGTPPTEEGKADLSYVEN
                     VTVEIAKSLTEMKKKKVDEKIYRLIVEKSTVPVLTSEWVKKTLELLTPTGTEFDIAAN
                     PEFLREGSAVNDFFYPDRIIIGVESEKGKKIFEEIYAPIKCPRIFTDIKSAELIKHAS
                     NSFLALKISYINAIANICEKVGADVDVVAEGMGLDKRIGKEFLKAGVGYGGSCFPKDV
                     SAFIHICEELGYPFDLLKEVQKINHQQRLLIIKKAKELLWNLKDKTIGIFGLSFKPKT
                     DDIRESPAIEIINLLKKENAKLKCCDPKAIENMKKIFPDLFYSEDPYETANGCHLLIF
                     LTEWEIFKNLDWKKIKEIMKMPYIIDGRNFLDYKNLRNIGFVYRGIGRKNE"
     gene            complement(4854..5888)
                     /locus_tag="PLF90_04385"
     CDS             complement(4854..5888)
                     /locus_tag="PLF90_04385"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOK56551.1"
                     /translation="MKKIIITGGAGFIGTNLSLYLLKDKNNYVVVYDNLSRKGVLKNL
                     EYLQSLKLKNFEFVKGDIRDYKKLNEVVKKSDIIYHLAAQVAVTKSVEDPIDDFEVNS
                     TGTLYLLEAIRKNCPDAVLIFSSTNKVYGELSHLKLKEGKKKYFLADRKNGIDEKENL
                     DFHSPYGCSKGTADQYIRDYYRIYGIKSVVFRQSCIYGSFQYGNEDQGWVAHFIIKAI
                     LGEKINIYGDGKQVRDILEVSDLINAYQLAIENIEECKGEIFNIGGGVRNTFSLLELI
                     EYLEKKLNKKIEVEFFDWRPGDQKVFISNNKKLEKYTGWNVKIDKYKGVDNLIRWVNE
                     NLQIIKEVLK"
     gene            5991..7046
                     /locus_tag="PLF90_04390"
     CDS             5991..7046
                     /locus_tag="PLF90_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56552.1"
                     /translation="MEPKERVESVLRKSKPDKIPFTIYENKLPQCTVERILRNNGLCI
                     LRRTSVYKTAYPDLSFKTITYTENGITYTKTLIETPFGELETTQRKTSQTGDFTIWKI
                     THLFKTPDDYKKINFMVKNAKYIPDYEKFIELERESGGDVFLRGNIALTPLHQIMIDF
                     MGIEKFSEEWAERRDEIENLYKNMVEKLRELYPILAESPCFAFNFGGNETGNVMGRGR
                     FERYVLPLYYEAAEILHKKGKVFGPHLDGNNKVWADLIAKSPLDYIEAFSPYPDTDMK
                     FEEGYNLWKDKILWINFPSSLHLASEEKIKEFLYEIIEISKNDFRLIIGITEDIPRER
                     WQKNLLLISEVINNFPI"
     gene            7066..8334
                     /locus_tag="PLF90_04395"
     CDS             7066..8334
                     /locus_tag="PLF90_04395"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK56553.1"
                     /translation="MEIEKIKEKLLEKMENLEIIDAHEHLPPERIRIEKETDVFILFS
                     HYTHGDLLRAGMSEEQYKSLFNHEIPLEKRWKIFLPFWKKIRYTSYSKAVLLSLKKFY
                     GFDDINDGNYYEISEKIKEFNKKGIYKKVLRDTCKIKFALTQCGTTETESEILIPLMP
                     LNKNGEMDTLENLTKSPYNEKNRIKAIDDLIEEYKKYILKSKENGAVGFKTMAFPFGI
                     PDRKKARDLFNRIMKGKIKKIEAPVLPPSIIGTNPIFDYVHDEIIKFVGEQNLVIAVH
                     TGYWGDFRRLSPENFIPVLKRHPEVKFDIYHCGFPYVREALNLGKEYSNVYLNFAWTH
                     IISQKFAIDALDECFDLIPVNKIIAFGGDYNLPVEKVYGHLVMAKEDVATVIAKRIKE
                     KIMSFEDGFEVIKNIFFENPKNLYNLTKRN"
     gene            complement(8338..9156)
                     /locus_tag="PLF90_04400"
     CDS             complement(8338..9156)
                     /locus_tag="PLF90_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK56554.1"
                     /translation="MKLKIGVIPDCFRLPIKEGIKKAAELGLDGIQPYATHGDLDPKN
                     LTKTGRDDLKHFVESLGLVISALVGDFGGHGFADPKTVEWRIERTKEVIDLAYDLGVK
                     VVTTHIGVVPEDENDIAWKTMEESLPEVGTYAYDKGIVLATETGPEPAVLLKKLLDKI
                     GNPGLKVNYDPANLVMVAGDDPVKGVYTLKDYIVHTHAKDGIKLPDENGKKRWKELPL
                     GEGGVNWPEYLKAMKEIGYNGFFTIEREVGEKPEVDIIKARDFLRKLEKEIWSK"
     gene            complement(9153..9380)
                     /locus_tag="PLF90_04405"
     CDS             complement(9153..9380)
                     /locus_tag="PLF90_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOK56555.1"
                     /translation="MKYIIKVNKERCKSCRLCIEICPSGVFTLSENFNCMGYHYVEVV
                     NGKNCIGCKRCVIICPDVAIEIYKNKKKEEI"
     gene            complement(9377..10156)
                     /locus_tag="PLF90_04410"
     CDS             complement(9377..10156)
                     /locus_tag="PLF90_04410"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOK56556.1"
                     /translation="MFERIFILENKKITGIAKEKEKLENLLKSCGKTVEKKIKNPDLI
                     LTMGGDGTILKAIGLLKSPKTIIYGINYGKVGFLTNSSENIEEKIKKVLDGKFKISER
                     ILLELKIKKGNKAVYKGKVLNDFLVFRKGIRIVEIEVFIDEKSVFNFRGDGIIISTPT
                     GSTAHSLSAGGPVIFPDNECILLTPFCPYSLNTRPLIIPSDKIVKIKISPDGKVIGDG
                     QKEYDVEKDELIEIKKSEIKAKLIIEDNFSEKLKSKFNFGK"
     gene            complement(10149..11969)
                     /gene="dxs"
                     /locus_tag="PLF90_04415"
     CDS             complement(10149..11969)
                     /gene="dxs"
                     /locus_tag="PLF90_04415"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOK56557.1"
                     /translation="MKLLEKIKDDYPLIKDLSIEQLNQLCEEIREFIIEVVSKNGGHL
                     SSNLGTVEITVALHKVFNIPPDKIIWDVGHQSYTHKILTRYEKFETLRKYGGISGFPN
                     PGESETDIFLVGHAGTGISSATGIKIGNDFKEKKEQIIAVIGDGSLTNGITFEGLNFL
                     GDIKKNVKIILNDNKMSISPTKGALSYYLTKFISSPLINKPKEEFVEILKKIPALGDD
                     ILKITKELEKKTKFLIVPGVFFEKLGIRYFGPIDGHDIEKLIEILENIKEINEPVVLH
                     VITKKGKGYSFAESDPEKFHSIGPFDIKTGNITGTQKTNSKFVGEILEKLAEKNNFFV
                     ITAAMEKGLGLENFARNYQDRFFDVGIAESNAVIIASGIAKSGMNVFVAIYSTFLQRT
                     YDQIFHDICLQNLPVIFLVDRAGIVGEDGPTHHGIFDISFLRTIPNLKIYAPYSLETL
                     KETIENSIYQKMPTFIRYPRDILPETINEEINEGSSVVILAVGSMALNSYFAFEKLKK
                     EKIILSFIPVNQIKPVDEVLMKKIEKFDKIITVEENITSGGFGSYLLEIFSDRNIKKY
                     IFRIGLPETFIEHGDRNLLLDKYGLSVEKIYEKIREFLNV"
     gene            complement(11941..13311)
                     /locus_tag="PLF90_04420"
     CDS             complement(11941..13311)
                     /locus_tag="PLF90_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK56558.1"
                     /translation="MKKYLVLTGLLLILTGCITPNLKKDIALKTYLSGLIYEDEKDNT
                     SAIKEFEETIKKGGPDPYIYIKIGNLYMKENNYKKAKENFQKASNLNPDIPEPYFGLG
                     LANLLEKKYKEASIYIEKGLKIKPDSSSFRLILCDIYSKLNNFNMAEHHYKILIEEYP
                     DNYLLHYNLAKIYEAQGNYKEAEKMYLGILEKRPNFWEGYLSLGILYQKENKIEQAKE
                     YLEKSLQFNPFNKLAYSLLAVIYYSEKNDEKTKEILLKAIERGLKEKEFYDFLGSIYM
                     GEKNYEIAETYWVESLKIEDSSDTRFKLGVLYDKKGDFEKMEENMKKAIELNPENSFA
                     LNYLGYSYLLKDKNLNEAFEMIKKACKIEPNNGAYLDSLGWAYYKLGDYKNAKKYLEK
                     AVKLEKDPEIYEHLGYLYFQLKDFEKSILYFSKVYEETGKKEILEMIEKVKNMLKNET
                     TGKNKR"
     gene            complement(13417..14289)
                     /gene="hisG"
                     /locus_tag="PLF90_04425"
     CDS             complement(13417..14289)
                     /gene="hisG"
                     /locus_tag="PLF90_04425"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOK56559.1"
                     /translation="MNKLKLVLPKGSLQETTLLIFKNAGFEIYSSSRSYYLTIDDPEI
                     ECVLLRTQEIPRYVELGAFDAGIAGKDWIIENNAKVIEVCELTYSKMGFRPVKIVLAA
                     PEKSNIKTVKDLEGKIIATELVNLTKRYLKKNRVNAKVEFSFGATEIKAGNLVDAIVE
                     ITETGSTLYSHNLKIIDVIMESTPRLIANRNSWKDKWKREKIENIALLLKGALQAEGK
                     VGLKMNVEKKNLENIIKILPAMKKPTISTLTDKNWYAIETIIDKNKAKELIPKLKKLG
                     AQGIVEYPLNKVII"
     gene            complement(14282..15256)
                     /locus_tag="PLF90_04430"
     CDS             complement(14282..15256)
                     /locus_tag="PLF90_04430"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOK56560.1"
                     /translation="MMEKKYLPFEKPIEEIEKKIEEIEKSKTKNTVEKNTEILKLKQL
                     LNEKKKEIYSNLTPQQIVQVARHPNRPHTLDYLNNIFDEYIELHGDRIFKDDPSIVSG
                     FGKFSGITVAFVGHQKGRDTKENIERKFGMAHPEGYRKAIRIMKLAEKFIVPVITFID
                     TPGAHPDIEAEERGQALSIATNLYEMALLKIPILCIIIGEGGSGGALGIGVGDRILMQ
                     EFSIYSVISPEGCASILWKTQEKVEEAAKALKLTAKDLFELKIIDEIIPEPTGGAHSD
                     WETTFKIMKEVIERNLKELKTIKVEELVELRYKKYRKIGVFSEGERSE"
     gene            complement(15253..>15480)
                     /gene="gatC"
                     /locus_tag="PLF90_04435"
     CDS             complement(15253..>15480)
                     /gene="gatC"
                     /locus_tag="PLF90_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOK56561.1"
                     /translation="KEEEKDKFEKELEKILEYVSKLNEVNTENVTPSYHVLPIKNVFR
                     EDIPDKSTSKEEILKNAPDRDENFFKVPRII"
BASE COUNT         5026 a         2529 c         1890 g         6035 t
ORIGIN      
        1 tttttgataa tttaaatttt gattttcctt ttttccttcc ataccatata gttggaactt
       61 cgcagatttt atatcctgaa aagaaaaact ttaaacatgc ctccatagat attgaaaaac
      121 cctttgattt taaattcaaa gaattcaaaa tagtccttct gtacatctta aaagcatttg
      181 ggcagtcctt tgttggtatc cctatcaggt aatataaact tactcctaca aatcttgaaa
      241 ataaactctg aatttttgga cctcctattt ttttgccttt tttggaatat cttgaaccgc
      301 atatcaggtc aaatccttcc tttgcttttt taaccatttt ttttatcgtt tctggctggt
      361 cacataaatc agccataaca ggtaaaacaa attctccttt tgcattttca aagccagttt
      421 ttaaagtgtt tgcaaatcca ggttctttct cgtttcttac aatttttaaa aaattatatt
      481 ttttacagag ttgggaaaca atattttcag ttctatcttc tgagtggtcg tttactacaa
      541 ttatttcagt ttcttctata tttatattag ggattatgct ttctattgtt tcttttatat
      601 tttcttcttc atttcttgct ggaataatta tactcaaaaa taaatccata gattttaaac
      661 aagccctttc tttctcaatt cttcttgtgc cttttcaaac ttatcctctg cctctgtttc
      721 ttttgcccat tcaatcagtt ttttcatccc ttcttcaagt gtggtctgtg gttcaaatcc
      781 aatcttattt tttattttac ttatatcagc ataacagtgt cttacatctc cttttctgaa
      841 tttataagtt atctctggtt ttaaatcctt accgagtagt tttattaaaa gttctgccat
      901 ttcaagtata gaaacaggtt ttccacttcc aacattaaaa atttggtaat ttgcttcatt
      961 tttttccatt gataaaatac aggcattaac aacatctcca acccagataa aatctcttgt
     1021 ctgtaatcca tcttcataaa taacaggagg attattattc tttattctgc ttaaaaaaat
     1081 tgcacagaca ccggtataag gatttgaaag tgtttgtctc ggtccataaa cattgaaaaa
     1141 tcttaaagaa acaacaggaa ttccgtatgt ttttccaatc agaagagaca tttcttcctg
     1201 ttctttttta cttatggcat aaattgagtt acttataagg ggtaatgtct caggagtagg
     1261 taagggtgtt attttttctt tacagtaagg acattttggt tcccagtcat ttattttttc
     1321 gtctttaaaa tctcttaaat ttggttttac atttccacat ttttcacatt tataaagtcc
     1381 ttctccataa atactcattg aagaagcaac aataagtttt tttacattat tttttgtatt
     1441 tacaaggata tccagtaaat tcgctgtacc ttttatattt acatcaacat atttttctat
     1501 ctgatactga gattgcccca caccaacctt tgcggcaaaa tgaaaaatat aatcaatttc
     1561 ctttattatc tttttcaatt cctgtttttt tcttatatct tcaaaaatat accttgcatt
     1621 taaatttata taatcaggta taccatcagg atgaacctgt gggtctaaac tgtcgtaaac
     1681 agtaacctca tatccttttt ttacaagttc gtcaacaaga tgacttccaa taaatcctgc
     1741 accacctgta acaagaactt ttttcataat acttaattta ccagaaatta aaacaaaaat
     1801 caaaaattga aatttaaata acaaggtaat ataattattt aaatggcaaa agaaataaat
     1861 tttttaaaag aaagaagcaa agagttttgg aatgagggag ttcgtcttta taaagaaggc
     1921 aaatataatc tttctgtttt caatatagaa caggcaattc aattatggat aaagtatttg
     1981 ataggaatta aaattactga ttggcctaaa acacactatt tttctgaatt aataaaaagt
     2041 ttgtcggatg tttatgagaa aaaagggata attgaatatt ataaaaagaa tgagatattt
     2101 tttaataacc ttgaagatgc ttattttaca acaagatatt tcccaaaaaa tttttcaaaa
     2161 aatattgcaa atcaatttat agaaaacgca aagaaattta tattgtttac tgaaaattta
     2221 acaggggaga aattttttaa tgagtaaaag tttagtagat atattaaaaa aaagggcaga
     2281 ggagaaggag aagtatttta aaaattatct tttttattgc aaaaaaataa aagaagaggc
     2341 agaaaaaata ttagggaaag tagaggtaat tgtttttggt tctgttgtaa aaaatgaata
     2401 tactccttta agcgatatag atgttttaat tatttcagat aatttgccta attcatacga
     2461 agagaggata aaaattaaaa ctgaaataaa atcaaaaatt gactctttct ctccttttca
     2521 aatccatctt tcaaactata aggaatataa taactggtat aaaaaattca ttaaaaacga
     2581 ctttatgaag atttagaaag gcgaatataa tattcaattg ttttttttag cccttcttca
     2641 agtttaattt gtggttccca gttgagtaat ttttttgcaa gtgttatatc aggttttctt
     2701 tttcttgggt catctggaag aggtggaagg aattcaaatc caagtttttt gccaaatatt
     2761 ttttctattt tttcagcaag ttggcttatt gtaaattctt caggatttcc gagattaatg
     2821 ggtaaattat aatctacttc aagaactttt ataattcctc taattaaatc atcaatataa
     2881 caaaaacttc ttgtctgatt tccgtctcca taaattggta atggtttatt gtttaaagca
     2941 ttatatataa aattcggtat aactcttccg tcattaagtc gcattctcgg tccataggta
     3001 ttgaaaattc ttattatttt ggtatttaac ttatacttac ggtgataagc cataacgatt
     3061 gcctctccat atctttttcc ttcatcataa acacttctcg gaccaattgg attaacattt
     3121 ccccagtact cttcattctg aacaggtata agcgggtctc cataaacttc agaagtagaa
     3181 gaaaataaaa aagtagattt tgattttaaa gcaatattaa gcattttttc agtaccaata
     3241 gaatttactt tcagtgtttc aatcggatat ttcatataat caacaggtga agcaggactt
     3301 gcaaaatgta aaacataatc aaatttttcc tttatttcat cttcattaag ataaattagg
     3361 tccttttcaa taaaaacaaa atttttgtta tttaagagat gttttatatt tgtttttgtg
     3421 gatgttataa agttatcaat acaccagaca gtatatccat tattaacaaa aaaatcacac
     3481 aaatgacttc caacaaaacc cgctcctcca cttattaaaa tttttttatt cattttttct
     3541 ccctatccct ctgtaaacaa atccaatatt ccttaaattt ttataatcaa gaaagttcct
     3601 accatcaatt atataaggca tcttcattat ttcttttatt tttttccagt caagattttt
     3661 aaaaatttcc cattctgtta aaaaaattaa aagatgacag ccatttgcag tttcataagg
     3721 gtcttctgaa taaaataagt caggaaatat ctttttcata ttttcaattg cttttgggtc
     3781 gcaacatttt aattttgcat tttctttttt taataaattt attatttcaa ttgctggtga
     3841 ttctcttata tcatctgttt tgggtttaaa agaaagtccg aaaattccta tagttttatc
     3901 ctttagattc catagtaatt ctttcgcttt ttttattata agtaacctct gctgatgatt
     3961 tattttttgg acttctttta aaaggtcaaa gggataacca agttcctcac agatatgtat
     4021 aaatgcagaa acatcttttg gaaaacaact tccaccatat ccaacacctg cttttaaaaa
     4081 ttctttacct attcttttgt caagtcccat cccttctgca actacatcaa catcagcacc
     4141 tactttttca cagatatttg ctatagcatt tatataagaa atttttaaag caaggaaaga
     4201 atttgaagca tgtttaatca attctgcact ttttatatca gtaaaaattc tgggacattt
     4261 tattggagca tatatttcct caaatatttt ttttcctttt tcactttcaa cacctattat
     4321 aattctatca ggatagaaaa aatcatttac agcactgcct tctcttaaaa attctggatt
     4381 tgcagcaata tcaaattcag ttcctgttgg agttaataat tccagtgttt tttttaccca
     4441 ttcacttgtc agaacaggaa cagtactttt ttctacaatt aatctataaa ttttttcatc
     4501 aacctttttc tttttcattt ctgtaagtga ttttgctatt tcaacagtaa cattttctac
     4561 ataagataaa tctgcttttc cttcttctgt tggtggagtt ccaactgcta tgaatattat
     4621 ttctgaattt tcaacagttt cataaattga gtcagtgaag gttaacagat ttttttcaac
     4681 tccttcttta acaatttctt caagacctgg ttcatagaaa ggcattatca aatttttaag
     4741 ttttttaact ttttcaacat cattatcaca gcatataact ttatttccaa gttttgaaaa
     4801 gcacgcacct gtagtaagtc caacatgacc agcaccaatt attccaatat ttctcatttc
     4861 aaaacctctt ttattatttg taagttctca tttacccatc ttatcaagtt atcaacacct
     4921 ttatatttat caatttttac attccagcca gtatattttt caagtttttt attattactt
     4981 ataaatactt tctggtcacc tggtctccag tcaaaaaatt caacttcaat ttttttattt
     5041 aatttttttt caaggtattc aatcaattca agtaaagaga aagtattcct aactccacct
     5101 ccaatattaa aaatttctcc tttacattcc tcaatatttt ctatagccag ttgatatgca
     5161 tttataaggt cagaaacttc aagaatatcc cttacctgtt ttccatctcc atatatattt
     5221 attttttctc caagaattgc cttgataata aaatgagcaa cccagccctg gtcttcatta
     5281 ccatactgaa aagaaccata aatacaagat tgcctgaaga caacactttt tattccgtaa
     5341 attctatagt aatcccttat atactggtca gcagtccctt ttgaacagcc gtatggagag
     5401 tgaaaatcaa ggttttcctt ttcatctatt ccattttttc tatcagccag aaaatacttt
     5461 tttttaccct cttttaattt taaatgactt aattctccat atactttatt tgtggaactg
     5521 aaaattaaaa ctgcatcagg acaatttttt cttattgctt caagtaaata taaagttcca
     5581 gtggaattta cttcaaagtc atcaataggg tcttcaacag attttgtaac agcaacctgt
     5641 gctgcaagat gataaataat atcggatttt ttaacaactt catttaattt cttataatcc
     5701 cttatatctc cttttacaaa ttcaaaattc tttaatttaa gagattgtaa atattcaaga
     5761 ttctttaaaa ctccttttct tgaaaggtta tcgtagacaa ctacataatt atttttatct
     5821 tttaacaaat ataatgagag gtttgtccct ataaatccag caccacctgt aatgattatt
     5881 tttttcatac agttttagtt tatatctttc tattctttat gtcaaccttc atttctgatt
     5941 ttttctataa ttgattgact tgaaatttaa tgtatgtaaa ataaaatata atggaaccaa
     6001 aggaaagagt tgaatctgta ttaagaaaga gtaaacctga taaaatacct tttacaattt
     6061 acgaaaataa attacctcaa tgtacagtag aaagaatttt aaggaataac ggattatgta
     6121 ttttaaggag aacttctgta tataaaactg cctatccaga tttatccttt aaaactatta
     6181 catatacaga aaatggaatt acatatacta aaacattaat tgaaacaccc tttggagaac
     6241 ttgaaacaac tcaaagaaaa acctctcaaa caggagattt tacaatatgg aagattacac
     6301 atttatttaa aacccctgat gattataaaa aaataaattt tatggttaaa aatgctaaat
     6361 atattcctga ttatgaaaaa tttattgaac ttgaaagaga aagtggtggt gatgtttttt
     6421 taaggggaaa tattgcttta acacctttac atcagataat gattgatttt atgggaattg
     6481 aaaaattttc tgaagaatgg gcagaaagaa gagatgaaat tgaaaattta tataaaaata
     6541 tggttgagaa attaagagag ttatatccta tacttgcaga atcaccatgc tttgctttta
     6601 attttggagg taatgaaaca ggaaatgtta tgggtagagg aagatttgaa agatatgttt
     6661 tacccttata ttatgaagca gcagaaattt tacataaaaa agggaaggtt tttggtcctc
     6721 atcttgacgg aaataataag gtctgggcag atttaattgc aaagtcaccg cttgattata
     6781 tagaagcatt ttccccttat cctgatacag atatgaaatt tgaagaaggg tataatttgt
     6841 ggaaagataa aattttatgg ataaattttc catcttcact tcatcttgct tcagaagaaa
     6901 aaataaaaga attcctctat gaaatcattg aaatttcaaa aaatgatttc agattgatta
     6961 tcggaattac agaagatatt ccaagagaaa gatggcagaa aaatttactt ttaatttctg
     7021 aagtgataaa taactttcca atttaatttt gaaacggagg aaattatgga aatagaaaaa
     7081 ataaaagaaa aattgctgga aaaaatggaa aatcttgaaa ttattgatgc acacgaacat
     7141 ttaccacctg aaagaatccg aatagaaaaa gaaacggatg tttttattct tttttctcat
     7201 tatacacatg gagacctttt aagagcaggt atgtcagaag aacaatataa atcactcttt
     7261 aatcatgaaa taccacttga aaaaagatgg aagatttttt tacctttctg gaaaaaaatc
     7321 agatatactt cctattcaaa ggcggtttta ctttcattaa aaaaatttta tggatttgat
     7381 gatataaatg atgggaatta ttatgaaatt tctgaaaaaa ttaaagaatt taataaaaag
     7441 ggaatatata aaaaagttct gcgggataca tgtaaaataa aatttgcatt aactcaatgc
     7501 gggacaactg aaacagaatc tgaaatttta attccactta tgccactcaa taaaaatgga
     7561 gaaatggata cattggaaaa tttaacaaaa tccccgtata atgagaaaaa cagaataaaa
     7621 gcaattgacg accttattga agaatataaa aaatatattt taaaatcaaa agaaaatgga
     7681 gcagtaggat ttaaaacaat ggcttttcct tttggaattc ctgatagaaa aaaggcaaga
     7741 gatttattta acagaattat gaaaggaaaa attaaaaaaa tagaagcacc tgttttaccc
     7801 ccttcaatta taggaacaaa tcctattttt gattatgtac atgatgaaat tattaagttt
     7861 gttggtgaac agaatttagt aattgcggtt catacaggtt attggggtga cttcagacga
     7921 ctttctcctg aaaattttat tcctgtttta aaaagacatc cagaagttaa atttgatatt
     7981 tatcattgtg gttttccata tgttagagaa gcacttaatc ttggtaagga atactcaaat
     8041 gtttatctta attttgcatg gacccatata atttctcaga aatttgcaat tgatgctctg
     8101 gatgaatgtt ttgatttaat tcctgtaaat aaaattattg catttggtgg agattataat
     8161 ttacctgttg aaaaagtata cgggcattta gtaatggcaa aagaagatgt ggcaactgta
     8221 atagcaaaaa gaataaaaga aaaaattatg agttttgaag atggttttga agtaataaaa
     8281 aatatctttt ttgaaaatcc gaagaatctt tataatctaa caaaaagaaa ttaattttta
     8341 tttactccaa atttcctttt caagttttct taaaaaatct cttgctttaa ttatgtcaac
     8401 ttctggtttt tctccaactt ctctttcaat agtaaaaaat ccattatatc caatttcttt
     8461 cattgctttt aaatattcag gccagttaac tccaccttct ccaagaggta attccttcca
     8521 tcttttctta ccattttcat caggaagttt aatcccgtct tttgcatgtg tgtgaacaat
     8581 ataatccttt aatgtatata ctccttttac agggtcatct cctgcaacca ttacaagatt
     8641 tgcagggtca taatttactt taagaccagg atttcctatt ttatcaagaa gtttttttaa
     8701 taaaaccgcg ggttccggac cagtttctgt ggcaagaact attcctttat cataagcata
     8761 tgtacctact tctggaagag attcttccat cgttttccat gcaatatcat tctcatcttc
     8821 aggaacaact cctatatgtg tagttacaac tttaactcca aggtcataag caaggtctat
     8881 tacttccttt gttctctcta ttctccattc aactgttttt gggtcagcaa aaccatgccc
     8941 tccaaaatca cctacaagag cacttataac aagccctaaa ctttcaacaa agtgttttaa
     9001 atcgtccctt cctgtctttg ttaaattttt tgggtctaaa tcaccatggg ttgcataagg
     9061 ttgaatacca tcaagaccaa gttctgctgc tttctttatc ccttccttaa ttggcaatct
     9121 aaaacaatcc ggaataactc ctatttttaa cttcatatct cctccttttt tttgttttta
     9181 tatatctcaa ttgcaacatc tggacaaatt ataacacacc ttttacaacc aatacaattt
     9241 tttccattta caacttcaac ataatgatag cccatacaat taaaattttc tgatagagta
     9301 aaaacaccag aaggacatat ttctatacat aatctgcaac ttttacatct ttctttattt
     9361 acttttataa tatatttcat tttccaaagt taaattttga ttttaatttt tcagagaaat
     9421 tatcttctat tatcaatttt gccttgattt cacttttttt tatttctatc aattcgtctt
     9481 tttctacatc atattccttc tgtccatctc ctataacttt tccatcaggt gaaatcttaa
     9541 ttttaacaat tttatcagaa ggaataatca atggtcttgt atttaaagaa tagggacaga
     9601 aaggagtaag taaaatacat tcattatcag gaaaaattac aggacctcct gctgaaagag
     9661 aatgagcagt tgaacctgtt ggagtagaaa ttattatccc atctcctcta aaattaaata
     9721 cacttttttc atcaataaat acttcaattt caactattct tattcctttt ctaaaaacta
     9781 aaaaatcatt aagtacttta cctttatata ctgccttatt tcctttttta attttaagtt
     9841 caagtagtat cctttctgaa attttaaatt ttccgtctaa aactttttta attttctctt
     9901 caatattttc agaagagttt gttaaaaagc caacttttcc ataattaata ccataaatta
     9961 ttgtttttgg acttttcaga agtccaattg cctttaaaat tgtaccatct ccacccattg
    10021 ttaaaattaa gtcgggattt tttatttttt tttcaacagt ttttccacaa ctttttaaca
    10081 ggttttcaag tttttctttt tcctttgcaa tccctgtaat ttttttattt tcaagtataa
    10141 aaattctttc aaacatttaa aaactccctg attttttcat aaattttttc aactgaaagt
    10201 ccgtatttat caagaagtaa atttctatca ccgtgttcta taaaagtttc aggtaatcct
    10261 attctaaaaa tatacttttt tatattccta tccgaaaaaa tctcaagtaa gtatgaacca
    10321 aaaccaccag atgtaatatt ttcttctaca gttataattt tatcaaattt ttcaattttt
    10381 ttcattaaaa cttcatcaac aggtttaatt tgatttacag gtataaaact taaaataatt
    10441 ttttcttttt ttagtttttc aaatgcaaag tatgaattta aagccatact tccaactgcc
    10501 agaataacta cactactacc ttcatttatt tcttcattta tagtttccgg taaaatatct
    10561 cttggatatc tgataaatgt tggcattttt tgataaattg aattttcaat agtttctttt
    10621 aaagtttcta aactgtaagg agcataaatt ttaagatttg gtatagttct taaaaaagat
    10681 atatcaaaaa ttccatgatg tgttggtcca tcttctccaa ctattccagc cctgtcaaca
    10741 agaaaaatta caggaaggtt ttgcaggcaa atatcatgga aaatctggtc atatgttctc
    10801 tgtaaaaagg tactgtaaat tgcgacaaaa acattcattc cacttttagc aattccagag
    10861 gcaataatca cagcattact ttcagcaatt ccaacatcaa aaaatctatc ctgataattt
    10921 ctggcaaaat tttcaagtcc aagtcccttt tccattgctg ctgttattac aaaaaaatta
    10981 tttttttctg caagtttttc aagtatttct cctacaaatt ttgaatttgt tttttgagtt
    11041 cctgttatat tacctgtttt tatatcaaaa ggtcctattg aatgaaattt ttcagggtct
    11101 gattcagcaa aactataacc ctttcctttt tttgtaataa catgtaaaac aacaggttca
    11161 ttaatttctt ttatattttc aagaatttca atcaactttt caatatcatg gccatcaatt
    11221 ggaccaaaat atcttatacc aagtttttca aaaaatactc caggtacaat cagaaatttt
    11281 gttttctttt ccagttcttt tgttattttt aatatatcat ctcctaaagc aggaattttt
    11341 tttaaaattt ctacaaattc ttcttttggt ttattaatga gaggggaact tataaatttt
    11401 gttaaataat aagaaagtgc tccttttgtt ggtgaaatag acattttatt atcgtttaga
    11461 ataattttta cattcttttt aatatctcca agaaagttta atccttcaaa agtaatacca
    11521 tttgttaatg aaccgtctcc tataaccgca attatttgtt cttttttttc tttaaaatca
    11581 tttcctatct taatccctgt tgcagaagaa atacctgttc cagcatgtcc aactaaaaaa
    11641 atatctgtct cactttcacc tggatttggg aaaccactta ttccaccata ttttctcaat
    11701 gtttcaaatt tttcatatct tgttaaaatt ttatgagtgt aagattgatg tcctacatcc
    11761 catatgattt tatctggagg aatattgaaa accttatgaa gagcaactgt tatttctact
    11821 gtgccaagat ttgatgaaag gtgtccacca tttttagaaa ccacttcaat aatgaattct
    11881 cttatttctt cacataactg atttaattgt tcaatggata ggtcttttat aaggggataa
    11941 tcatctttta ttttttccag tagtttcatt ttttagcata ttcttgactt tttcaatcat
    12001 ttccagaatt tcttttttgc cagtttcttc atacactttt gaaaaataaa gaatagattt
    12061 ttcaaagtcc tttaattgaa aatacagata tccaagatgt tcatatattt cagggtcttt
    12121 ttcaagttta actgcttttt caagatattt ctttgcattt ttatagtccc cgagtttata
    12181 ataagcccat cccaaactat caagataggc accattattg ggttcaattt tacatgcttt
    12241 ttttatcatc tcaaatgctt cattaagatt tttatcttta agaaggtaag aatatccaag
    12301 ataatttaaa gcaaaagaat tttcaggatt aagttctatt gcttttttca tgttttcttc
    12361 catcttttca aaatcaccct ttttgtcata taaaacgcct aatttaaatc ttgtatctga
    12421 tgaatcttca atttttaaac tttcaaccca gtatgtctct gcaatttcat aatttttttc
    12481 acccatatag attgaaccaa gaaaatcata aaattctttt tctttcaggc ctctttcaat
    12541 tgctttcaat aatatttctt ttgttttttc atcatttttt tcgctgtaat atataaccgc
    12601 aagaagtgaa tatgcaagtt tattaaatgg attaaactgt aaactttttt caagatactc
    12661 ttttgcctgt tctattttat tttctttctg ataaaggatt cccagagata aatatccttc
    12721 ccagaaatta ggtcgttttt ctaatattcc taaatacatt ttttccgctt ctttgtaatt
    12781 tccctgtgct tcataaattt tagccagatt ataatgtaaa agataattat caggatattc
    12841 ttcaataaga attttataat gatgttccgc catattgaaa ttatttaatt ttgaataaat
    12901 gtcacataaa atcaatctaa aagaactact atcaggtttt attttaagtc ctttttcaat
    12961 atatattgaa gcctctttat attttttttc aagtaaattt gctagtccca gaccaaaata
    13021 aggttcagga atatcaggat ttaaattgga tgctttctga aaattctcct ttgccttttt
    13081 ataattgttt tccttcatat ataagttgcc aatttttatg taaatatatg ggtcaggacc
    13141 tcctttcttt attgtttctt caaattcttt aatagcagaa gtattatctt tttcatcttc
    13201 ataaataagt cctgaaagat atgtttttaa agcaatatct tttttcaaat taggagtaat
    13261 acatcctgta agaattaaaa gtaaacctgt taataccaga tattttttca tatcattttc
    13321 tctttttatg tctcattgct tttcttcttt ttttgtactt atgtctttta acctttttta
    13381 ttttcctttt tcttccgcaa ggcatactcc ctccctttaa ataattactt tgtttaatgg
    13441 atattcaaca attccctgag caccaagttt tttcaatttt ggtattaatt cttttgcttt
    13501 atttttgtca attattgttt caatagcata ccagttttta tcggttaaag ttgatattgt
    13561 cggttttttc attgctggta aaatttttat aatgttttca agattctttt tttcaacatt
    13621 catttttagt cctactttcc cttctgcctg taaagcacct tttaataata atgctatatt
    13681 ttcaattttt tctcttttcc atttgtcctt ccatgaattt ctattggcta tcaatcttgg
    13741 agttgattcc ataataacat caattatttt caagttatga gaatataaag tagagcctgt
    13801 ttcagtaatt tcaacaatag catctacaag atttcctgct ttaatttcag ttgctccaaa
    13861 tgaaaattcc actttagcat ttactctgtt tttctttaaa taccttttag ttagattgac
    13921 cagttcagta gcaataattt taccttcaag gtcttttaca gttttaatat ttgacttttc
    13981 aggagcagca agtactattt taacaggtct gaatcccatt tttgaataag tcaattcgca
    14041 tacttcaata acttttgcat tattttcaat tatccagtcc tttcctgcaa ttcctgcgtc
    14101 aaaagcacca agttctacat atctgggaat ttcctgagtt cttaataaaa cacattcaat
    14161 ttcagggtca tctattgtca gataataact tcttgaactt gagtatattt caaaaccagc
    14221 atttttgaaa atcaataaag ttgtttcctg taaagaccct tttggtaaaa caagtttaag
    14281 tttattcact tctctctcct tctgaaaaaa cacctatttt cctgtatttt ttatatctca
    14341 attcaacaag ttcttcaact tttattgttt ttaattcttt taaatttctt tctattactt
    14401 ctttcattat tttaaaagtt gtttcccaat cactgtgcgc tcctccagta ggttcaggta
    14461 ttatttcgtc tattattttg agttcaaata aatcctttgc tgttaacttt aatgcctttg
    14521 ccgcttcttc cactttctcc tgtgttttcc aaaggattga agcacatccc tctggagaaa
    14581 taactgaata tattgaaaac tcttgcatta aaattctatc gccaactcca ataccaagtg
    14641 ctcctccact tccaccttca cctattatta tacacaaaat tggaattttt aaaagagcca
    14701 tttcataaag atttgttgct attgaaagtg cctgtcctct ttcttccgct tctatatcag
    14761 gatgtgctcc tggtgtatca ataaaagtaa taacagggac tataaatttt tcagccagtt
    14821 tcataatcct tattgctttt ctataacctt ccggatgagc cataccaaat tttctttcaa
    14881 tattttcttt tgtatctctt cccttttgat gaccaacaaa agcaactgtt attccagaaa
    14941 atttaccaaa accacttaca atagaagggt catctttaaa tattctatca ccatgtaatt
    15001 caatatattc atcaaaaata ttgttaagat aatctaatgt atgaggtcta tttggatgtc
    15061 ttgccacttg aacaatttgt tgtggagtca gattagaata aatttctttc ttcttttcat
    15121 ttaataactg ctttaatttt agaatttcag tatttttttc tactgtattt ttagttttac
    15181 ttttttcaat ttcttcaatt tttttttcaa tttcttcaat tggtttttca aaagggaggt
    15241 attttttctc catcatatta tcctcgggac cttgaaaaag ttttcgtccc tgtcaggagc
    15301 attttttaaa atttcttctt ttgatgtgga tttatcaggt atgtcttctc taaatacatt
    15361 ttttataggt agaacatgat aagaaggagt aacgttttcc gtgtttacct cattcagttt
    15421 tgaaacatat tcaagtattt tttcaagttc cttttcaaat ttatcttttt cttcttcttt
//
LOCUS       DAOSAT010000037        15430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2622_length_15430_cov_5.386537, whole genome shotgun sequence.
ACCESSION   DAOSAT010000037 DAOSAT010000000
VERSION     DAOSAT010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15430
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2622_length_15430_cov_5.386537"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PLF90_04440"
     CDS             complement(<1..751)
                     /locus_tag="PLF90_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOK56562.1"
                     /translation="MKAAVFYGIEDIRVENVEEPVCKENEIKIKVSYCAICGTDVRIF
                     YSGHKKVIPPAIIGHEITGIVAEIGKNVGYPEIKVGDKVTVVTSIGCGKCKICKEGFY
                     NLCPDTKAIGYYYKGGFAEYLIIPEDAVKQKAVLKLPENVSLLEGTIIEPLSCCINGQ
                     NYLNVKKDDFVLIFGGGPIGFMHASLAKAVGCKNVVIVDPAYERLLKFGKNFPDIILW
                     NPNKINLKEDVMKMTDGFGADVVITACPAKKA"
     gene            complement(764..1129)
                     /locus_tag="PLF90_04445"
     CDS             complement(764..1129)
                     /locus_tag="PLF90_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HOK56563.1"
                     /translation="MEVKIKFEREEIECEIFETELGKKIIDALPFSSKARLWGKEIYF
                     EIPVSSEIKNPKNEVEKGDIGYWPDGKCMCLFFGPTPISKGDKIIPASSVEIIGKIKS
                     DLKILENVKSGEKIKVEKK"
     gene            1200..2168
                     /locus_tag="PLF90_04450"
     CDS             1200..2168
                     /locus_tag="PLF90_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK56564.1"
                     /translation="MVKIGIIGCGGMGRYHANILKKFEDVKIISASDVNLDTLNSFKN
                     EFGVEEIYDDFNDLIKKSDVNAIICATPTNLHYKIVVKAAKAKKHIFCEKPISVTLKQ
                     AEKMIEECEKNKVILQIGYVRRFDDEWLKFKELMDKKIIGRPVVWHQISGGYGPSPEW
                     FYDIKNGMGPFVDGAVHSYDFAIFTFGKVKKVKSSLTKFKKFTSPDTGIVSVEFESGD
                     ILVLCWSWGLPKNTTCQHIHQALGPYGVLIFDGFRDDKGRYFVIKKENEEEKIGPIPL
                     NSLGIGFEKQMEHFIDCVKNNKKPIVDGIAGYESLKVGLEAIKNWK"
     gene            complement(2172..4517)
                     /locus_tag="PLF90_04455"
     CDS             complement(2172..4517)
                     /locus_tag="PLF90_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOK56565.1"
                     /translation="MKNYNFKSKLEEIAIEKFEKKPLYGYEKIKVLQVPNFPLLGKVV
                     SLRFIEWLQLNPEGVISLPTGKTPEYFIKWTKFYLKNWKELEVKKELNSWGIDVKNKP
                     DLKNYWFVQMDEFYPMDPKRENSFNYYIKKFYIEGFGFNSEKTILMNTWTIGVKGEKN
                     LSYIFPDGKVDLTLRYRMPKNELEKKQQEAIYEIDRYAMEYERKIEELGGIGFFLGGI
                     GPDGHIAFNIRGSDHNSTTRVLKINYETAAAAATDLGGIENSRDKAVMTIGLLTITKN
                     PTTTAIIMAAGESKASVVRDAIENEPDILYPATSLQKLVGARFYLTSGAASLLKGRKI
                     ENLKKYKSIPYSEKEKILVDISYRLDKKITDLTMNDIKNDIYGSVLIEKGEIRNLKKI
                     ILEIESNFKKRIQRGIQEIENTTFLHTAPHHDDIMLGYLPYILHLVRKPTNYHFFATL
                     TSGFTSVTNNYLFEQLENLEKFIKNGKLKRLISEKKYFSKDNITGRNRDIYRYLDGVA
                     AQNEEIRREAEARRMYRNIVEIIKNDKPEEVLDKVLFLKKEILKAYPGKKDIPDIQKL
                     KGMIREWEEELLWAHLGFSCNNIFHLRLGFYTGEIFTPQPEWERDVKPFLEILKRIKP
                     DVLTVAFDPEGTGPDTHYKVLQIIAQSVKKYVSEVNKNIKIWGYRNIWDRFHPSEANI
                     YVPVSMNSIAIMKSAFDICFGSQRSASFPSYEYDGPFSELAVKIMVQQGGFIKVILGR
                     DFFGGSEYARIRASRGVVFIKEMDVDEFLTESSNLKSIMED"
     gene            complement(5017..5388)
                     /locus_tag="PLF90_04460"
     CDS             complement(5017..5388)
                     /locus_tag="PLF90_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56566.1"
                     /translation="MEKALMFLSFFLGFLSVVMAFVLSYNTNKLVRTEDERAKKIIDE
                     GNQRAQELIKETQKMIEEGNKRTQEMIERMNIAHREMLERMDARMEKMDDTLRFLGTL
                     IKSEGEKTRELINKVLEKMPG"
     gene            complement(5627..6133)
                     /locus_tag="PLF90_04465"
     CDS             complement(5627..6133)
                     /locus_tag="PLF90_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56567.1"
                     /translation="MNKEEKRYRVLKSFFYGWISIIVVNEIMRRLLISKFLDEQRKFL
                     TTYPTQLLDTLVIYFSFSLCQKHLISSFLTFMIGSLIMAYFIGEKGWIYSIFLIFPAS
                     ALIQIYSFFAVDILKLTFKAKFLEVKTSLFLGLFTSLLVIFLSIVIGGFLGTSLKFIQ
                     LKVKDYKR"
     gene            complement(6148..6402)
                     /locus_tag="PLF90_04470"
     CDS             complement(6148..6402)
                     /locus_tag="PLF90_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56568.1"
                     /translation="MSKKYEEQLNYCKLLYGSRFFVNPEYLKCIGQAVANKKDCDEWA
                     KCRNGPPGPYNPQIQMSPLPWWFRLLPLIDYIPWRLIELF"
     gene            complement(6375..6746)
                     /locus_tag="PLF90_04475"
     CDS             complement(6375..6746)
                     /locus_tag="PLF90_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOK56569.1"
                     /translation="MKKYYDRLGSVRFMSDGSGNVVSEYVYDAWGNLVSSSGSLSQPY
                     QYVGEYGYYKEEDLGLYLLGQRWYDAEVGRFVSRDPIGERGGLNLYEYVGNNGVNSID
                     PEGLQNEDIRSELCQKSMKNN"
     gene            complement(6743..7036)
                     /locus_tag="PLF90_04480"
     CDS             complement(6743..7036)
                     /locus_tag="PLF90_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56570.1"
                     /translation="MEKAMMFLSFFLGFLSVVVAIVLPIVLGRRTDELIRTEDERAKS
                     LISETQKMIERMNIAHREMLERMDDTLRFLGTLIKSEGEKTRELINKVLEKMP"
     gene            7128..7430
                     /locus_tag="PLF90_04485"
     CDS             7128..7430
                     /locus_tag="PLF90_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56571.1"
                     /translation="MKFLSVIPPFHTSIPQRVLSILIVKFGYKTQYRNYLENLEKLVP
                     EILPSLPLDPFTGKEYVYKKKGNGFLIYSLGKNQKNDNGIYNLKKRADDIGFEILF"
     gene            complement(7437..8882)
                     /gene="amrB"
                     /locus_tag="PLF90_04490"
     CDS             complement(7437..8882)
                     /gene="amrB"
                     /locus_tag="PLF90_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOK56572.1"
                     /translation="MRKFLILFLLFASILLPAYRQPAFSGSFYPSNKEELKNMIDNFL
                     KNVNYKEKIEKGEIIGIIAPHAGYIYSGPVAAYSFKLLEGMDIKTVILIGRSHNSHFK
                     GGVIDDREGWEMPLGKVEIDKEIFDKLYKKKNFSVNKVLLDYEHSLEVEVPFLQVVLK
                     NFKIFPILLGDSSKENIKKIADDIYEILKDKKNWIIVASTDLSHYYPYETAKQKDGLL
                     LETLKTKNLNLLYSYLSTRKVEMCADAAVLTLFKIAEKYPDYEVKILNYANSGDTSGD
                     KSRVVGYGAIAILKKDKKGGNMLTENQKKLLLKIARETLENYLSGKKLPELKVDDPVL
                     VEKRGVFVTLKKGGNLRGCIGYIEGVEPLYKAVRTMAIHSATQDPRFEPVKYEELKDI
                     EIEISVLTVPQKVKSAGEIVLGRDGVIVKRGFNQGVFLPQVAEETGWTKEEFLSYLCS
                     HKAGLPPDAWKDPKTELYIFQAEVFSEKELK"
     gene            complement(8879..9961)
                     /gene="amrS"
                     /locus_tag="PLF90_04495"
     CDS             complement(8879..9961)
                     /gene="amrS"
                     /locus_tag="PLF90_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOK56573.1"
                     /translation="MQIYKGKIKKFIILLFLSSTLFAQNLKEASFYKKLDNKLVQCFL
                     CPKICIISPSKYGFCRARKNINGTLYSMGYSNPCSVAVDPIEKKPFFHFLPSTSTFSI
                     ASAGCSLRCKFCQNWEISQYSPEETRNIYLPPEKVVEYAIKYKCPSIAYTYSEPVNFY
                     EYMFDTAKIAKEKGIKNIVHTAGFINPEPLEKLCKYLDAVNVDLKGFNKDYYRNVCEG
                     ELDVVLNTLKILKKNKIWIEITNLIVPGYNDKPEEIKNMCLWIKDNLGPETPLHFSRF
                     HPMYLMLHIPSTPVETLQMAVKIAKNSGLKYVYIGNVEGSEYENTYCPKCGKLLIKRY
                     GFSVLENNVVNGKCKFCGEKIEGVWK"
     gene            complement(9925..10374)
                     /locus_tag="PLF90_04500"
     CDS             complement(9925..10374)
                     /locus_tag="PLF90_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOK56574.1"
                     /translation="MIKFITDGMCGGLARWLRLIGYDTLYFNTPRKIEIIRTAEKENR
                     IVLTKDKKLVNAYPEIVFYIEGENTEKQFEEVVKKFKLEIKEENLFKICSLCNTKLEK
                     IEKEKVKNLVPEFVYNNKEEFAICVKCNKIYWEGDHCRYIRERLKNL"
     gene            complement(10380..10451)
                     /locus_tag="PLF90_04505"
     tRNA            complement(10380..10451)
                     /locus_tag="PLF90_04505"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10417..10419),aa:Met,seq:cat)
     gene            complement(10464..11060)
                     /gene="rdgB"
                     /locus_tag="PLF90_04510"
     CDS             complement(10464..11060)
                     /gene="rdgB"
                     /locus_tag="PLF90_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOK56575.1"
                     /translation="MKLYFYLATKNKNKVREIKNIVENEIEVLLPPDFLGFPEETGNT
                     LEENAYLKAEYLSKVIKDEYVVGEDSGLFVEKLNGLPGVNSARFSGERDDKKNIEKLL
                     KMMEELKNKEDRKAKFITVACLVGKGEKKFFKGEIEGYITFTPRGSNGFGYDPVFEIP
                     ETGKTFAELSLEEKNKISHRTKAFLKLKEYLIKKEEKR"
     gene            complement(11032..11772)
                     /gene="rph"
                     /locus_tag="PLF90_04515"
     CDS             complement(11032..11772)
                     /gene="rph"
                     /locus_tag="PLF90_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOK56576.1"
                     /translation="MEIRRKGNRPYDELREIKITKGFLKYAAGSCLIEMGDTKVICAV
                     SYEDKVPQWLKDTGNGWITAEYSLLPSSTQERIPRSTLSSGRTHEIQRMIGRSLRGVV
                     DLKRIGERTFWVDCDVIQADGGTRVAGVIGGFIALVDCLNKLKNSGLISVPVLKDYIG
                     AVSVGIVHNNILLDLDYSEDSLASVDLNVVMTGSGEFIEIQGTAEGYPFTKKQLDHLL
                     TLAEKGISKIIEIEKEVLKNEIIFLLGH"
     gene            complement(11763..>13154)
                     /locus_tag="PLF90_04520"
     CDS             complement(11763..>13154)
                     /locus_tag="PLF90_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56577.1"
                     /translation="FYLPVFSLDLKAKKTFFLPSFEYKTRTGASLTFNFTNRFSEKKD
                     YLMNEKILIGEKRIGVGLGFSSEEKNINFNSFFLKKYDGEILPGGYLEIQRNFKNLDF
                     LVDWRWMDNNEFFIDFFRDEYLRKSKMFNYFSITDYLGKGILGFTIRENAQEDVLKVE
                     KIPEIRYFLPYFNFSDIPLFLTYDFRFTNFHKEGENCLRILNKIDLMYKKDLPYFTVK
                     PYISFSSLNYPDMDLYKFNYIGEGGINFSTNFYSKFSDVVFVPSISFFKRETRYNPEQ
                     LIKFDEFEEKNSGIFLSSSLLWNINLYKGLTGNFRVENDYNFDKNKFEDISLKYELAF
                     KSFKIEGDNEWILRENSSYYNFGVNTVSYEAEKYKFSIGTRVDDESDIFGLETWYQQS
                     LKNDWNFRIGVFYDFDSKDLISQTYELWKKIHCFVIDFRFVKDIENKAFYVFVVPSIF
                     FENNWQRRFEKWK"
     assembly_gap    13155..13254
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13255..13524)
                     /locus_tag="PLF90_04525"
     CDS             complement(<13255..13524)
                     /locus_tag="PLF90_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56578.1"
                     /translation="MKFKTFLIFFLLFSFSVLSEEIKYKGDNIKVFFGKEDEIERIEM
                     EGNVVIVYKDITIKTNRAVFDKKDNFIECEEGVEIFSSSGRFYAEK"
     gene            complement(13514..14458)
                     /locus_tag="PLF90_04530"
     CDS             complement(13514..14458)
                     /locus_tag="PLF90_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOK56579.1"
                     /translation="MKERVLCLGIIVGDFLVKPVEDMPERGKLTLVEKTELHIGGCAT
                     NTGIVLKKLGVDVAIVGKVGNDNLGKFIINKLKEEGINTDYIKISDKFTTSGTSVLVH
                     SDGERSFIHSIGANADFGLGDFDFSLLKNFSILHIAGPFLMPKFLKKGLSEVMKKAKE
                     EGLITCLDTVWDAFGNWFKGIEDALPYIDYLFTSFEEAKMISKKEDKEDICDFFLFHN
                     VKNVCLKMGEKGSYIANEKESYYFPALKVNVIDTTGAGDGYVAGFIKGITEKFEFKKC
                     GLLANIVGAKITTAIGTTAGIKNWEDLVKFAKEFGYEI"
     gene            14569..15312
                     /gene="cas6"
                     /locus_tag="PLF90_04535"
     CDS             14569..15312
                     /gene="cas6"
                     /locus_tag="PLF90_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOK56580.1"
                     /translation="MRFYIQFISNKEVKVPSNIRSFFISFIKKVFENTDKLLYSSLFN
                     SKKAKPYVFSPYFGEEFKEGKIGKDISFIFSSGDFSLISSFWNGILLLKEKKLDYIEI
                     EGEKFNLNNIKLLPNKKIIGNEVIFKTIGISVLTDPEVSAKDFKNWYIIPEKDKIERF
                     KDVLRKRTEERIKYIKGIEKKIELEFSIPEEATNSDTVVPHYDGYVRGFRGIFCLKGE
                     PEILQFLYDYGFGVRTGQGFGLLEIVKQI"
BASE COUNT         5102 a         2758 c         1744 g         5726 t
ORIGIN      
        1 gtgctttttt agcaggacat gcagtaataa caacatctgc tccaaaacca tctgtcattt
       61 tcataacatc ttccttcaag tttattttat tcgggttcca taaaattata tccggaaaat
      121 ttttaccgaa tttcaataac ctctcataag cagggtcaac aattacaaca tttttacaac
      181 caactgcttt tgcaagagat gcatgcataa atccaattgg tccacctcca aaaattaaaa
      241 caaaatcatc tttttttaca ttcaaataat tctgtccatt tatacaacaa gaaagtggct
      301 caataattgt tccttcaagt aatgaaacat tttcaggtaa ttttaaaacc gctttctgtt
      361 taactgcatc ttcaggaata attaaatatt cagcaaatcc acctttataa taataaccaa
      421 ttgcctttgt atcaggacat aaattataaa aaccttcttt gcaaatctta cactttccac
      481 aaccaattga agtaacaact gtaaccttat caccaacttt tatttcagga taaccaacat
      541 ttttccctat ttctgcaact atacctgtta tttcatgtcc tattatggca ggtggtatta
      601 cttttttatg ccctgaataa aaaatcctta catcagttcc acatatagca caataggaaa
      661 cttttatctt tatttcattt tccttacata caggttcttc tacattttca acccttatat
      721 cctcaatccc ataaaaaacc gctgctttca tttttttctc ctttcatttt ttctcaactt
      781 ttattttttc tccacttttt acattttcaa gtatttttag gtctgatttt atttttccta
      841 ttatctcaac agaacttgct ggaattattt tatctccttt acttattgga gttggaccaa
      901 aaaataaaca catacatttc ccatcaggcc agtatcctat atctcctttt tcaacttcat
      961 tttttgggtt tttaatttca gaagatacag gaatttcaaa atatatctct ttaccccata
     1021 atcttgcttt tgaagaaaaa ggaagtgcat caattatttt tttcccaagt tctgtttcaa
     1081 aaatttcaca ttcaatttct tctctttcaa acttaatttt tacttccata ccttcctcct
     1141 tttattttta ttttaacatt tttttgtgat atgatataaa aaccaaaaaa ggagaaaaaa
     1201 tggtaaaaat aggaataata ggttgtggtg gaatgggaag gtatcacgca aatattttaa
     1261 agaaatttga agatgtcaaa attatttctg cttctgatgt aaatttagat actttgaatt
     1321 cttttaaaaa tgagtttgga gttgaagaaa tatatgatga ttttaatgat ttaattaaaa
     1381 agtcagatgt taatgcaata atctgtgcca ctccaacaaa tctacattat aaaatagttg
     1441 taaaagcagc aaaagcaaaa aaacatatat tctgtgaaaa accaatatct gtaactttaa
     1501 aacaggcaga aaaaatgatt gaagaatgtg aaaaaaataa agtaattctt cagattggat
     1561 atgtgaggag atttgatgat gagtggttaa aatttaaaga attgatggat aaaaaaatta
     1621 ttggaagacc agttgtatgg catcagatat caggtggtta tggtccttca cctgaatggt
     1681 tttatgatat taaaaatggg atgggtcctt ttgttgatgg agctgttcat tcttacgatt
     1741 ttgctatttt tacatttgga aaggttaaga aagtaaaaag ttcattaaca aaatttaaaa
     1801 aatttacctc tcctgacact ggtattgttt ctgttgaatt tgaaagcgga gatattcttg
     1861 ttttatgctg gagttgggga cttcctaaaa atactacttg ccagcatatt catcaggcac
     1921 ttggacctta tggagtttta atttttgatg gtttcagaga tgataaagga agatattttg
     1981 taataaaaaa agaaaatgaa gaggaaaaaa taggtccaat tcctttaaat tctcttggta
     2041 taggatttga aaagcagatg gaacatttta ttgattgtgt aaaaaataat aaaaaaccaa
     2101 ttgtagatgg aattgctggt tatgaatcat taaaagtcgg tcttgaggca ataaaaaact
     2161 ggaaatgaat tttaatcttc cataatactc ttcaaattac tgctttcggt cagaaattca
     2221 tcaacatcca tttctttaat aaaaacaact cctctgcttg ctctaattct tgcatactca
     2281 cttccaccaa aaaaatccct tccaagaatt actttaataa atccaccctg ttgtaccatt
     2341 atctttacag cgagttcaga aaaaggtcca tcatattcat aacttgggaa agaagcactt
     2401 ctctgtgaac caaaacatat atcaaaagca cttttcataa tagcaattga attcattgaa
     2461 acaggaacat aaatatttgc ttcagaagga tgaaatctat cccatatatt tctgtatccc
     2521 catattttta tatttttatt tacctctgaa acatattttt ttacagattg agcaattatt
     2581 tgaagaactt tgtagtgagt atcaggtcct gttccttcag ggtcaaaagc aaccgtaaga
     2641 acatccggct taattctttt taaaatctca agaaatggtt ttacatctct ttcccattca
     2701 ggttgaggtg tgaaaatttc tcctgtataa aatccaagtc gtaaatgaaa aatattatta
     2761 caactgaacc caagatgtgc ccataaaagt tcctcttccc attcccttat cattcctttt
     2821 aacttttgaa tatcaggaat atcttttttc cctggatatg cttttaaaat ttctttttta
     2881 agaaacaaaa ctttatctaa aacttcttct ggtttatcat tttttattat ctcaactata
     2941 ttgcggtaca ttctccttgc ttctgcctct ctccttattt cttcattttg agcagcaact
     3001 ccatcaaggt atctgtatat atctctattc cttccagtaa tattatcctt tgaaaagtat
     3061 ttcttctctg aaatcaacct ctttaatttt ccatttttta taaatttttc aagattttca
     3121 agttgttcaa acagataatt atttgttact gatgtaaaac cactcgttaa tgttgcaaaa
     3181 aaatgatagt ttgttggttt tcttacaaga tgaagtatat atggtaagta tccaagcatt
     3241 atgtcatcgt gatgtggtgc agtatgtaaa aatgtagtat tttcaatttc ctgaattcct
     3301 ctctgtattc tctttttaaa attactttct atttccagaa ttattttttt taaatttctt
     3361 atttctcctt tttctatcag aacagaacca tagatatcat tttttatatc attcatagtt
     3421 aaatctgtaa tcttcttatc caatctgtag gatatatcca caagtatttt ttccttttca
     3481 gaataaggaa tagatttata ttttttaaga ttctcaattt ttcttccttt caaaagagat
     3541 gcggctccgg atgttaaata aaatcttgca cctaccaatt tctgaagtga agttgcagga
     3601 taaagtatat caggttcatt ttcaatagca tctcttacaa cagatgcctt actttcacca
     3661 gcagccataa taattgcagt agtagtagga tttttggtaa tggttaaaag tccaatagtc
     3721 ataactgctt tatctcttga attttcaatt cctccaaggt cagttgcagc agctgccgct
     3781 gtttcataat taatttttaa aactcttgtt gtagaattgt ggtcagaccc tcttatattg
     3841 aatgctatat gtccatcagg tcctattccg ccaagaaaaa atccaattcc accaagttcc
     3901 tcaattttcc tttcatattc catcgcatat ctatcaattt cataaattgc ttcctgttgt
     3961 tttttttcta attcattttt tggcattcta tatcttagag taaggtcaac ttttccatca
     4021 ggaaaaatat aagaaagatt tttttctcct ttcactccga tagtccatgt attcataaga
     4081 attgtctttt cagaattaaa tccaaaacct tcaatataaa acttttttat ataataatta
     4141 aaactgtttt ctctttttgg gtccattgga taaaattcat ccatttgaac gaaccagtaa
     4201 tttttcaaat caggtttatt tttaacatca attccccatg aatttaactc tttttttact
     4261 tccagttctt tccagttttt taaataaaat tttgtccatt ttataaaata ttcaggagtt
     4321 tttcctgttg gaagagatat aactccttct gggtttaatt gtagccactc aataaatctt
     4381 aatgaaacaa ctttgccaag taaagggaaa ttaggaacct gaagaacttt tattttctca
     4441 tatccataaa gaggtttttt ttcaaatttt tcaattgcta tttcttctaa ttttgattta
     4501 aaattataat ttttcatatt tcacattcta atcgcgtatt taaatataaa ctacttactt
     4561 tttaatccca aaaaataccc tcttaaagtt aacttgccat caataaataa ttctattttt
     4621 acagtatagg aatgtggaac tttggatatt ttttgatttt tcagagtgat gatagatgtc
     4681 ataaattaaa aagatgagaa atataataat taaaaaagta ataattatgg accataatat
     4741 aataaacgga gtatctcctg caggatgtgt ccacaaagaa cttttacaat aaggacattt
     4801 tatccacatt cttaaaaaac agaataaaaa caatgttttt ttacttatca aaaattcttt
     4861 tttacattta aaacaataaa cttgttgcca ccttacttct tttaatttgt ctattagatt
     4921 catttcaata attttaaaat catttatttt aaaacatatt tacactttca tctatcataa
     4981 actgtttgtc tctaatttgg tcataacctt tcttaatcat cctggcattt tctcaagtac
     5041 tttgtttatt aattctcttg tcttttctcc ttctgatttt attaatgtgc ctaaaaacct
     5101 caatgtatca tccatcttct ccattctcgc atccatcctt tcaagcattt ctctgtgggc
     5161 tatattcatc ctctctatca tctcctgtgt tcttttgttc ccttcttcta tcatcttttg
     5221 tgtctcttta attaattctt gtgccctctg gtttccttca tctataattt tctttgctct
     5281 ctcatcttct gtccttacta atttattcgt attataactt aaaacaaatg ccataactac
     5341 actcaaaaac ccaagaaaaa aacttaaaaa cattaatgcc ttttccattt tcttcccctt
     5401 tctttttatt atactcctct tttatcccat ttggcaagtg tttctctatt aaatttccca
     5461 aattggtaaa tttaattaaa ataataatta aataaaattc tgattatata acatgcgtta
     5521 aaaaatgaaa atttaaactt tccaaatcca atctattttc caaaagttgt taaactctca
     5581 atttgatttt gtttttattt gttaacttct tttatcaaaa tttgctttat cttttataat
     5641 ctttaacttt taattgtata aattttaaac ttgtgcctaa aaaaccacca attacaatac
     5701 ttaaaaatat tacaagaaga gaggtaaata gacctaaaaa tagcgaagtt ttaacttcta
     5761 aaaattttgc cttgaaggta agtttcaaaa tatcaacagc gaaaaaagaa tatatttgaa
     5821 ttaaagcact tgctggaaat attaaaaata tagaatatat ccatcccttc tctcctataa
     5881 aataagccat tattaaacta cctatcataa aggttaaaaa agaactaatt aaatgctttt
     5941 gacaaagaga aaaactaaag tatataacta aagtatctaa tagttgagtg ggatatgtag
     6001 ttaaaaattt tcgttgttca tccaaaaatt tagatattaa aagccttctc attatttcat
     6061 ttacaacaat tatacttatc catccataga aaaaagactt taaaacccga tatcttttct
     6121 cttccttatt catttttatc aaatttatca aaataattca atcaatctcc aaggaatata
     6181 atctatcaag ggtaataaac gaaaccacca gggtaatgga gacatttgaa tttgtggatt
     6241 atatggtccc ggtggaccat ttctacattt agcccattca tcacaatctt ttttatttgc
     6301 aactgcttga ccaatgcatt taagatattc aggattaaca aaaaatctac ttccatatag
     6361 taatttacaa taattcaatt gttcttcata cttttttgac aaagttcact ccttatatct
     6421 tcattttgta aaccttctgg atctatacta ttcactccat tattccctac atactcatac
     6481 aaattcaaac cacctctctc acctatcgga tctctactta caaacctacc tacttctgca
     6541 tcataccacc tctgacctaa taaatacaaa cctaaatctt cctccttata ataaccatac
     6601 tctcctacat attgatacgg ctgacttaaa ctacctgaac tacttactaa attaccccaa
     6661 gcatcataaa catactcact aactacattt ccactaccat cactcataaa tctaacacta
     6721 cctaacctgt cataatactt cttcacggca tcttctcaag tactttgttt attagttccc
     6781 ttgtcttttc gccttctgat tttattaatg tacctaaaaa cctcaatgtg tcatccatcc
     6841 gttcaagcat ttctctgtgg gctatattca tcctctctat catcttttgt gtctcactta
     6901 ttaaactttt tgctctctcg tcttctgtcc ttattaattc atctgtcctt cttcctaaaa
     6961 ctatgggtaa aacaatagca actactacac tcaaaaaccc aagaaaaaaa ctcaaaaaca
     7021 tcatcgcctt ttccattttc ttcccctttt cttttattat actgttcttt tatccttttt
     7081 ggcaagttca tgaattcctg attaagtgaa aaagcacagc gcttgtaatg aaatttttat
     7141 ctgttatacc tccttttcat acttctatcc cacaaagagt tttatcgatt ttaatagtta
     7201 agttcggtta taaaacacaa tacagaaact atcttgaaaa tcttgaaaaa cttgttcctg
     7261 aaatattacc atctttacca cttgacccat tcacaggaaa agaatatgtt tataagaaaa
     7321 aaggaaatgg gtttttaatt tacagtttag gaaaaaatca aaaaaatgat aatggaatat
     7381 ataatttaaa aaagagagcc gatgatattg gttttgaaat tctgttctga aattttttat
     7441 tttaattctt tctcactgaa aacctctgcc tgaaaaatat aaagttctgt cttcgggtct
     7501 ttccatgcat caggtggaag tcctgcttta tgagagcata gataacttaa aaattcttct
     7561 tttgtccagc ctgtttcttc tgcaacctga ggcaaaaata caccctgatt aaatcctctt
     7621 ttaacaataa ctccatccct tccaagaaca atctcacctg cactttttac tttttgagga
     7681 accgtaagta ctgaaatttc aatctctata tccttcagtt cttcatattt tacaggttca
     7741 aatcttgggt cttgagttgc tgaatgtata gccatagtcc ttaccgcctt ataaagtggt
     7801 tcaacgcctt ctatatatcc aatacagccc cttaaatttc ctcctttttt cagtgtcaca
     7861 aatacacctc ttttttcaac taaaacaggg tcatccactt ttaattcagg cagttttttc
     7921 cctgataaat aattttcaag tgtttctctt gctattttta aaagtagttt tttctggttt
     7981 tctgtaagca tatttcctcc ttttttatcc tttttcaaaa ttgcaattgc tccatatcca
     8041 acaactctgc ttttatcacc acttgtatct cctgaatttg cataatttaa tatcttgact
     8101 tcataatcag gatatttttc agcaatttta aataaagtta aaacagcggc atccgcacac
     8161 atttcaactt ttcttgttga aagatatgag taaagtagat ttaaattttt tgtctttagt
     8221 gtctcaagta ataatccgtc tttttgcttt gctgtttcat agggataata atgggataaa
     8281 tctgtacttg caactataat ccagtttttt ttatctttca aaatttcata tatatcatct
     8341 gcaattttct taatattttc ttttgatgaa tctccaagta aaatcggaaa aattttaaaa
     8401 tttttcagaa caacctgtaa aaatggaact tcaacttcaa gagaatgttc ataatcaagt
     8461 aatactttat ttactgaaaa atttttcttt ttataaagtt tatcaaaaat ctctttatct
     8521 atttcaactt ttcccaaagg catttcccat ccttccctgt catcaatcac tcccccttta
     8581 aaatgactat tatgacttct tccaattaaa ataacagttt ttatatccat gccttcaagt
     8641 aatttaaatg agtaagcagc aacaggccct gaataaatat aaccggcatg tggagcaata
     8701 ataccaataa tttccccttt ttcaattttt tctttataat ttacattttt taaaaagtta
     8761 tcaatcatgt ttttcaattc ttctttgttt gaaggataaa atgagccaga aaaagcaggt
     8821 tgtctgtagg caggaagtaa aatagaggca aaaagtaaaa acaaaatcag aaactttctc
     8881 atttccatac tccttctatt ttttctccac agaatttaca ttttccattt acaacattat
     8941 tttcaagaac agaaaatcca tatcttttta tcaatagttt accacatttg ggacagtatg
     9001 tattctcata ttcagaacct tcaacatttc ctatgtagac atatttcaat ccagaatttt
     9061 ttgcaatttt aacagccatc tgaagtgtct caacaggggt tgaaggaata tgaagcatta
     9121 aatacattgg atgaaatctt gaaaaatgta atggtgtctc cggtccaaga ttatccttta
     9181 tccataaaca catatttttt atttcttctg gtttatcatt atatccggga acaattaaat
     9241 ttgttatttc aatccagatt ttatttttct taagaatttt taatgtattt aaaacaacat
     9301 caagttctcc ctcacaaaca tttctgtaat aatccttatt aaaaccttta aggtcaacat
     9361 taacagcatc aagatattta cacaattttt caagtggctc tggattaatg aaaccggcag
     9421 tatgtacaat attttttatt cctttttctt ttgctatttt tgctgtatca aacatatatt
     9481 cataaaaatt taccggttca gaataagtat aggcaattga gggacattta tattttattg
     9541 catattcaac aactttttca ggtggtaaat aaatatttct tgtttcttca ggtgaatact
     9601 gtgatatttc ccagttctga cagaatttac atcttaaaga acagccagca gatgcaattg
     9661 aaaatgtaga agtagaaggt aaaaaatgaa aaaagggttt cttttcaata gggtcaactg
     9721 caacagaaca tggatttgaa tagcccattg agtacaaagt cccatttata ttctttcttg
     9781 ctctacaaaa accatattta ctaggtgaaa taatgcatat cttaggacat agaaaacact
     9841 gaacaagttt attatcaagt tttttataaa aagatgcctc ttttaaattc tgtgcaaaaa
     9901 gtgtagaaga cagaaataaa agtattataa attttttaat ctttccctta tatatctgca
     9961 atggtctccc tcccaataaa ttttattaca ttttacgcat attgcaaact cctctttatt
    10021 attatacaca aattcaggga ccaaattctt aactttttct ttttctattt tttcaagttt
    10081 tgtattacat aaagagcata ttttaaatag attttcttct tttatttcaa gtttaaattt
    10141 tttaacaacc tcttcaaact gcttttctgt attttctcct tctatataaa aaacaatttc
    10201 tggataggca tttacaagtt ttttatcttt tgtcagtaca attctatttt ctttttcagc
    10261 agttcttata atttcaattt ttcttggagt gttgaagtat aatgtgtcat aaccaatcaa
    10321 tctcaaccac cttgcaagac caccacacat cccgtctgtt atgaatttaa tcatatttgt
    10381 ggcgggatgg ggatttgaac cccagacata ccgggtatga gccggctgct ctgccactga
    10441 gctaccccgc caatttattt attttacctc ttttcttcct ttttaatcaa atattccttt
    10501 aacttcaaaa atgcttttgt tctatgactt atcttatttt tttcttccaa actcaattct
    10561 gcaaatgttt ttcctgtttc aggaatttca aacacagggt catatccaaa accatttgaa
    10621 cctcttggag taaaagttat atatccttct atttctcctt taaaaaattt tttttctcct
    10681 ttaccaacaa ggcatgcgac cgttataaat tttgcttttc tatcttcttt attttttaat
    10741 tcttccatca ttttaaggag tttttctata ttttttttat catctctttc acctgaaaat
    10801 cttgctgaat taactcctgg aagaccattt aatttttcaa caaaaagtcc agaatcctct
    10861 ccaacaacat actcatcctt tataacttta ctcaaatatt ctgcttttaa ataagcattt
    10921 tcttcaagcg tatttcctgt ttcttcagga aatcccaaaa aatcaggtgg caaaaggact
    10981 tctatttcgt tttcaactat attttttatt tctcttactt tatttttatt tttagtggcc
    11041 aagtaaaaat ataatttcat ttttaagcac ttctttttct atttcaatta tcttacttat
    11101 gcccttttct gcaagcgtta aaagatggtc aagttgtttt tttgtaaatg gataaccttc
    11161 cgcagttccc tgtatttcaa taaactcacc acttcctgtc ataactacat ttaaatcaac
    11221 agaagcaaga gaatcttctg aataatcaag gtcaagcaaa atattattat gaacaatccc
    11281 gacactcaca gcaccaatat aatcctttaa aacaggtaca cttattaaac cagaattttt
    11341 taatttattc aagcaatcca caagtgctat aaatccacca ataactccag ccactcttgt
    11401 tcctccatct gcctgaatca catcacaatc aacccagaaa gttctttctc caattctctt
    11461 taaatcaacc actcccctta aagaccttcc tatcatcctc tgaatttcat gagtccttcc
    11521 tgaagataaa gtacttctag gtattctttc ctgagtggaa gagggcaaaa gtgaatattc
    11581 agcagtaatc catccatttc ctgtatcctt caaccattga ggaactttat cttcataact
    11641 aacagcacat ataacctttg tatctcccat ttctattaaa cacgaaccag cagcatattt
    11701 taaaaaaccc tttgtaattt taatttctct taattcatca tacggtctat ttccttttct
    11761 tcttatttcc atttttcaaa cctcctttgc cagttatttt caaaaaatat agatggaaca
    11821 acaaaaacat aaaatgcctt attttctatg tcttttacaa atctgaaatc tatcacaaaa
    11881 cagtgtatct ttttccataa ttcatatgtt tgacttatta aatcttttga atcaaaatca
    11941 taaaaaaccc caattctaaa attccagtca ttttttaaag attgctgata ccatgtttca
    12001 agcccaaaaa tatctgattc gtcatcaact ctggttccta ttgaaaattt atatttttct
    12061 gcttcataag aaactgtatt tactccgaaa ttataataac tgctattttc tcttaaaatc
    12121 cattcattat ctccttctat cttaaaactt ttaaaagcaa gttcatattt taaagaaata
    12181 tcttcaaact tattcttatc aaaattataa tcattttcta ccctgaaatt tccggtaagt
    12241 cctttataca gatttatgtt ccatagtaaa gaagaagaaa gaaatatacc actgtttttt
    12301 tcttcaaact catcaaattt aattaactgt tctggattat atcttgtttc ccttttaaaa
    12361 aaagaaattg atggaacaaa aaccacatct gaaaacttac tgtaaaaatt tgttgaaaaa
    12421 tttatcccac cttctccaat ataattaaac ttgtaaaggt ccatatcagg ataattcaag
    12481 gaagaaaagg aaatatatgg ttttactgta aaataaggta agtccttttt atacattaaa
    12541 tcaattttgt taaggattct caaacagttt tcaccttctt tatggaaatt cgtaaatctg
    12601 aaatcatagg ttaaaaataa aggtatatct gaaaaattaa aatatggaag aaaatatcta
    12661 atttccggta ttttttcaac tttcaaaaca tcttcctgtg cattttctct tattgtaaaa
    12721 cctaaaattc cttttcccag atagtcagta attgaaaaat agttgaacat ttttgatttt
    12781 cttaaatatt catctctaaa aaagtcaata aaaaattcat tgttatccat ccatctccag
    12841 tcaactaaaa aatctaaatt tttaaaattt ctctgaattt caagatatcc accgggtaaa
    12901 atctctccat catatttttt aagaaaaaat gagttaaaat ttatattttt ctcttcggaa
    12961 gaaaaaccaa gacccactcc aatcctcttt tctccaatca atattttttc attcattaaa
    13021 taatccttct tttcactaaa tctatttgta aaattgaaag ttaaagaagc acctgttctt
    13081 gtcttatatt caaaagaagg aagaaaaaat gttttttttg ctttcaaatc aagtgaaaat
    13141 actggaagat aaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttttc
    13261 agcataaaat ctaccagaag aagaaaaaat ttcaacacct tcttcacatt ctataaaatt
    13321 atccttttta tcaaatactg ctctgtttgt tttaatcgta atatccttgt aaacaatcac
    13381 aacatttcct tccatctcaa ttctttctat ttcatcttct ttaccaaaaa aaactttaat
    13441 attatcccct ttatatttta tttcttcaga aagaactgaa aatgaaaaaa ggagaaaaaa
    13501 tatcaaaaaa gttttaaatt tcataaccga attctttcgc aaattttaca aggtcttccc
    13561 agtttttaat acccgcagtt gttcctattg ctgttgttat tttagcacct acaatattgg
    13621 caagaagacc gcacttttta aattcaaatt tttcagtaat tccctttata aaccctgcaa
    13681 catatccatc tcctgcacct gtagtatcaa tcacatttac ttttaatgca gggaaataat
    13741 aactctcttt ttcatttgct atatatgaac ccttttcacc catttttaaa cacacatttt
    13801 tcacattatg aaaaaggaaa aaatcacata tatcttcttt atcttccttt ttagaaatca
    13861 tctttgcttc ttcaaaactt gtaaataaat aatctatata agggagagca tcttctattc
    13921 ctttaaacca gtttccaaaa gcatcccata cagtatcaag acaggtaata agtccttctt
    13981 cctttgcctt tttcattact tcagaaagac ctttttttaa aaattttggc ataagaaatg
    14041 gtcctgcaat atgtaaaatt gaaaagtttt ttagtaaaga gaaatcaaaa tcccccaaac
    14101 caaaatcagc atttgcacct attgaatgaa taaaagacct ttcaccatct gaatgaacaa
    14161 gaacagaagt tcctgaagtt gtaaatttat ctgaaatttt tatgtaatct gtatttattc
    14221 cttcttcttt cagtttattt attataaatt taccgagatt atcatttcca actttaccaa
    14281 caattgcaac atcaacacca agttttttta aaacaatacc agtatttgta gcacaaccac
    14341 ctatgtgtaa ttcagttttt tcaacaagag ttaattttcc tctttccggc atatcttcaa
    14401 ctggctttac gagaaaatca ccaacaatta taccaagaca taaaactctc tctttcatcc
    14461 agcctccttt tttccattat cttaccatat attgatacaa aatagaagtg aaaaaatttg
    14521 aaaatttttt aattttatgg tatattatat attaaacaga aaccaaaaat gcgattttat
    14581 atacaattca tttcaaataa agaagtaaaa gttccttcaa acataagaag ttttttcatc
    14641 tcattcataa aaaaagtttt tgaaaatact gataaacttc tctactcttc tcttttcaac
    14701 tctaaaaaag caaaaccgta tgtattttct ccttattttg gtgaagaatt taaagaaggt
    14761 aaaattggca aggatatttc atttattttt tcaagtggtg atttttcttt aatttcaagt
    14821 ttctggaatg gaattttatt attaaaagaa aaaaaacttg attatattga gattgaagga
    14881 gaaaagttta atttaaacaa tattaaactt ctgccaaaca aaaaaataat cggaaatgaa
    14941 gttatattta aaacaattgg aatttcagtt cttacagacc ctgaagtttc agcaaaagat
    15001 tttaaaaact ggtatataat tcctgagaaa gataagattg agagatttaa agatgttttg
    15061 agaaaaagaa cagaagaaag aattaaatac ataaaaggga tagagaaaaa aattgaactt
    15121 gagttttcaa ttccagaaga agcaacaaat tccgatacag ttgttccaca ttatgatggt
    15181 tatgtaaggg gctttagggg gattttttgt ttaaaaggag aaccagagat acttcaattt
    15241 ttatatgatt atggatttgg agtaaggaca ggtcagggat ttgggctact tgaaattgta
    15301 aaacagatat aaaaatgaaa acttcaggaa aaatagaaag agtaaaaatt aaatataaac
    15361 agaaagtacc tatcccacct gaatacaaat cttttttctg ggactgtcag aatgaggtta
    15421 ttcttgaaaa
//
LOCUS       DAOSAT010000038        15399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2629_length_15399_cov_5.621611, whole genome shotgun sequence.
ACCESSION   DAOSAT010000038 DAOSAT010000000
VERSION     DAOSAT010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15399
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2629_length_15399_cov_5.621611"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /locus_tag="PLF90_04540"
     CDS             <1..1891
                     /locus_tag="PLF90_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK56581.1"
                     /translation="QVQNLKSGQPVSNDILNQPHPFLCQKCGFPLQTEESTCPNCLKQ
                     GAILSRVFSLLSEHIGWIFIIFFLMVIGVALSLIPPRITKILVDEVLTTRKHVDWLPY
                     LVIILVSAEFFRAQINMLVGTISTSVGTLITNNLRKKLFKKLEELSLSYYDINSVGTI
                     MTRFSSDVEAFHGFVTQAGQGFLLNIFMLIGIGIMLFSINWLLALYVMIPIPFVVIGT
                     LIFWRGVYPKYYKVWDSQAKLSTFLNNVLSGIKTVKAFSQEEKEFNRFTFYADKLKDS
                     VRTVNINISVFNPLMAFLFSLGGLIIWYVGGKNVLEGKLTLGELMAFLSYIGMFYSPL
                     TNLTLLSTWFSNFTTASHRIFEVLDTEPQVKEPKRAVKLMKIKGEIEFRNVTFGYDPY
                     QPVIKNVSFKIEPGEIVGIVGRSGSGKTTLVNLLCKFYDVQEGEILIDGKNLKDIPSY
                     ELRKHIGLVLQEPFLFRGTISENISYGKPDATFEEIINAAKVANAHEFIMKFPNGYDT
                     LLGERGAGLSGGEKQRITIARAVLCDPPILILDEATSSVDTESEKKIQDALSSLWKGR
                     TTIIIAHRLSTLKESDKIIVIDNGRIAEIGNHNELMEKRGIYYNLIVMQTQLSSIDGA
                     LIHGN"
     gene            1881..2396
                     /locus_tag="PLF90_04545"
     CDS             1881..2396
                     /locus_tag="PLF90_04545"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HOK56582.1"
                     /translation="MEIKKEYLTPDNIKILKGEFNTINLFLIEDGILYKSVFAVSCFP
                     VTNPYNFISIFYQKETGEIEEIGIIKNISVFPEEEKKLIIETLNKHYFYYEITRILDI
                     KWKFGFLLFKVETDKGEKEFYLRWERSKAIDIGKKGKLLIDIFEDRYIISDVEKLTPV
                     EKNLFTKFIYW"
     gene            2421..3311
                     /locus_tag="PLF90_04550"
     CDS             2421..3311
                     /locus_tag="PLF90_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOK56583.1"
                     /translation="MRTVAISMAYEYRKVRKIEDNLSYIENIFKDIKNINPDLVVLPE
                     VFAYVGIEFEKRDFGKWEKIKEFLKYIARKTNCWVCGSSYDLVDEKIYNSCFFVNRKG
                     DIVGKYDKIHPTEGEIEKGIYSGRKDQKPIETEFGKIGAFICFDANWHYDLKYQVNNG
                     AKLLVFSSAYPAGKILNSISLLYQVFIVAGIWSLNSGIIDNTGRWLVKTDRFSYWVWS
                     DINLERTVFHWDYQGDKVRKIIEKYKDGVKLETFGDEAIFTIEVVDRKISIEKIIKEF
                     KLVTYKEYLKRAEIMQNKKR"
     gene            3329..4159
                     /locus_tag="PLF90_04555"
     CDS             3329..4159
                     /locus_tag="PLF90_04555"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOK56584.1"
                     /translation="MIKLLTIDLDGTLLNNKGEINKEDKITLKKFYKSGGIVVLASGR
                     MTDCVSVYSEILGIDTPLIVYNGAMVRLTEKEKRKIIYHNPVPLKYSKFIINYCLENK
                     FLLNFYYNDTLYAVNDPLLKKYVDIYSKQTGAKYFFVDSLENFKNKKPTKLILITDTE
                     NENIKRTRDFQYRYFKKLFGKSLNIVKTNPEYLEFMNKGVNKGIALKALAKYYNIKPD
                     EIIAFGDGENDIEMLLYARISVVPVNAKEKVKKIAKIVSDYSNEDAYISKFLKGFLSI
                     "
     gene            complement(4162..5355)
                     /locus_tag="PLF90_04560"
     CDS             complement(4162..5355)
                     /locus_tag="PLF90_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOK56585.1"
                     /translation="MKVKNIESERIRNLPIYMFQAINAKKLQLRREGKDIIDFGMGNP
                     DKPPPAPVIEKLKEVLKDPKVHRYSASKGIFHLRKEIANWYLKRFGVKLDPEEEVVVC
                     IGSKEGISHLALAIFDKGDVVLIPNPTYPSHLYSAIISGATIYDLPLKKENNFIPDLN
                     QPIYDRFPKPKAMILSYPNNPTTQIVDIDFFKDVVKFAKKKDLIIIHDIAYSEIYFED
                     KDRPHSFLEVNGAKSIGIEFYSLSKTYNMAGWRVGFAVGNKYVIRALTKLKSYYDYGI
                     FTPIQVAAICALRLEEKYINEVRETYRKRRDVMVEGLNRIGWNVEKPKATLYIWAEIP
                     EKFKKMGSMKFSLYLLENAHVAVSPGAGFGKYGEGYVRLALVENEHRIKQAIRSLKNL
                     IKNKK"
     gene            complement(5345..6019)
                     /locus_tag="PLF90_04565"
     CDS             complement(5345..6019)
                     /locus_tag="PLF90_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOK56586.1"
                     /translation="MNLKGIVFDGDGVLFDTEKLHVIAWKTIFKFHNIELTEDDFAEG
                     VGVEDRVFLKNLKKKGKIKKIFKIEELVSEKNKQLLKIVNKKNLGVSEETKKILKFLK
                     NKYKIALASNSEKKFVLKVLKISDIYKFFDLVITRNDVKKPKPFPDIYTEVSRKLKIP
                     AKNIIAFEDSETGIISAKSSGIFCVALPSTQPLEKIKIADIIIHKLSLNNVKKVIKIF
                     EEKNES"
     gene            complement(6016..7137)
                     /locus_tag="PLF90_04570"
     CDS             complement(6016..7137)
                     /locus_tag="PLF90_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOK56587.1"
                     /translation="MFSKKKYFLYVSIFVSTFSLILLGTVFVLSSSFFYGLEKNNLIL
                     YFEKHLIYLFAGLFIFYLIQKSDIKKLEKYGLMILGISLIILPLPNIQGGLRWIKIGP
                     FSFQPSEFVKITFIIYLAIYLKKRISMVNNLKTLIIPIIIYLFIIAILQLQKDYGTFL
                     IISVTFLGVLFLCGLKSKYIFSISGFFIILMLGLILLSPYRIERIKLYLNKNEDPLGK
                     NFPPKQAKISLSSGGLFGKGLGAGTGKLKFVPEIHKDFVYAVVGEELGFAGSFGILLL
                     FSIIVFCGLEVSKFCNDLFEKILAFGISTSFGTQFLLHAGVVLSILPQKGTTLPFFSV
                     GGSSLLANLISLGILLKISENIINQDHSKDIEETILKLK"
     gene            complement(7103..8464)
                     /gene="murD"
                     /locus_tag="PLF90_04575"
     CDS             complement(7103..8464)
                     /gene="murD"
                     /locus_tag="PLF90_04575"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOK56588.1"
                     /translation="MNQIKGKKIGIIGLGISGFDTGIFLLENGGEVFISEKNVNEDIL
                     EKVNILKEKGAKIETGEHTPEFFREVEFVVISPGVDEKNEFIDFLKKRKIQFISEIEL
                     AYNFSKSKKIIAITGTNGKTTTTTLIGNIFKKANYPSVVCGNIGNTFIGEIKNIEEKT
                     WIILEVSSFQLEKIVNFRPYISCLLNIDYDHFDRYLNMEEYIQAKKRIFENQKENDFS
                     VLNFDDYHTKRLLKDIRSQKRFFSFSKISNGVYYENENIFLNLDNKNLKILETKNLKI
                     QGKGNIANIMASSLISILCGIEQETIKTAIENFEGLPHRMEKVAEIDGVIYINDSKST
                     NPHSVKNSVLSLKGKNNIILILGGKDKGFPYHQLTKYLKNRVKFIILLGEAKERIKKE
                     LASSNIPMEYVGNLKEAVILSKKVGKKGDFVLFSPGCSSFDMFKNYEERGDVFKKEVF
                     SLC"
     gene            complement(8461..9525)
                     /gene="mraY"
                     /locus_tag="PLF90_04580"
     CDS             complement(8461..9525)
                     /gene="mraY"
                     /locus_tag="PLF90_04580"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOK56589.1"
                     /translation="MLYKLLYSFTDLWFGFNIFKYVTVRATLATFTSLLLSLLFGRKI
                     INKLKNFCHPNYLRETEKEKTPTMGGFLILGTLLISTFFWADLKNTYIILLIFLTFSL
                     GFVGFIDDYTKLRYKSHKGVRPLFKIIVQMIVGFIIGLILVYKSDLNFNTSLYIPFFK
                     KAILPLGEYFILFAVFIIVATSNAVNLTDGMDGLAIGLILTVSLAYAIISYVVSNVKF
                     ANYLNVPFVKGAEEATVFSGALVGSCLGFLWFNCYPAEIFMGDIGSLPLGGIVGLLAI
                     IVKQELSLIFVGGIFLVEALSVIIQVISYKSRGKRIFLMTPIHHHFQLKGVSESKVVI
                     RFWIVGIILALFTIITLKIR"
     gene            complement(9540..10880)
                     /gene="murF"
                     /locus_tag="PLF90_04585"
     CDS             complement(9540..10880)
                     /gene="murF"
                     /locus_tag="PLF90_04585"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOK56590.1"
                     /translation="MEKVKLSEIIKWCKGKVNKKFKDFFIEGISTDSRQIKKGEIFIA
                     LKGEKYDGHDFVKDAFKKGAVASIVSSDYIGNDNLLIKVKDTLYALGDIGKNYRIKLN
                     MHVINITGSDGKTTTKEIIKEILSEKFNTTGTIGNFNNQIGLPLSILQADKKTEIGVF
                     EIGMNKKGEIDYLSKISAPNSCVITNIGVAHIGFFKNRKELADAKSEIFGNLIKEKNV
                     FLNKDSDFFYYLKGKTDTKNLITIGVKKNADITGEIIEEEFDYFIFEYSGEKYKMNFW
                     NTSFIYSGLFGIAFGKKFGISEKHIKNVLYGLKPLKGRGEFIKDDIFIIDESYNSNPN
                     SLKNSLLSFERKKFKRKIAIIGDMAELGKFSNFYHWYIGNLIRNLKIDIIFTFGKESK
                     KISEVSGKGKHFYEIDELKKELKREIKKQDAVFIKGSRIVKMDEIVDFLKTDFF"
     gene            complement(10882..12399)
                     /locus_tag="PLF90_04590"
     CDS             complement(10882..12399)
                     /locus_tag="PLF90_04590"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOK56591.1"
                     /translation="MKLKELIKDIKKKKVFNLNNFEVEGIAYDSRKIKENYVFIAIKG
                     TKQDGHNFINEAIERGAKCVIVSKRPSIIPADITEIVVEDTKKTLSEIANIYYDFPSK
                     KLNIVGITGTNGKTTTSFIIKEILNENGIKAGLIGTIFYEIGERKIVSTNTTPESLDL
                     IEMFSEMLKNGCKWAVMEVSSHGSEQRRIEGIDFNAGIFTNIASHEHLDYHKTFKNYL
                     NAKLLLFTRYLKDSEKKEKYGIINLDSPYAKVFIKGCEKNGIKWITFGKNRNSNVKLL
                     SYRVEKGGNYLEIAIENEKLFFNSKISGLGNVYNLLAGIAFSYIYKIPFETVQKAFLK
                     IENVPGRFEFIDEGQDFSVIVDYAHTHHALNNLLLSVKSLRPKRIILIFGCGGDRDKS
                     KRPLMGKIAVKMADFVIITSDNPRTEDPIKIIKDIERGIPFYLRKKYVAIPDRKKAIY
                     EGISMAKSNDCVVIAGKGHEAFQILKDVTIPFDDREEARKAIKEIINRKKEEKLL"
     gene            complement(12377..14125)
                     /locus_tag="PLF90_04595"
     CDS             complement(12377..14125)
                     /locus_tag="PLF90_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOK56592.1"
                     /translation="MKQKQDDIFERRIRYTKLIFFTGFLLIFCQLFNLQVIMYSKYKI
                     IERKTRYAKIKVLAERGKIYDRWGNLLAFNLPNYSLYFDTWMIQKLKEKNPEYLNELK
                     NKLSEILKIDKKQIEEKMNEPYALIKKDIEIEEYEKIKKTNLKGIYFERKYKRVYPYG
                     RHACHILGYTDIDGKGLEGVEYFYDSFLKGKDGIYLVLKDGLGNLIPSIKKTLIKEEK
                     GKDIVLTIDSDIQFIVEEEIKNGYEKFSPKNISVIVMDADTGEILALANYPDYDPNNF
                     SKFDLSIIRNKCVTDLFEPGSVFKIVTAAAAIEENIFTPDSTIMCEYGKWFVRDHYLH
                     DVHPYGILTFEETVVKSSNIGIVKIAMSLGEEKLYKYCKKFKFGERTGIDLPGEIGGI
                     FRPLENWSSYSITAIPIGQEVGITSIQGIRAISVFANGGYLVKPHIIKEIKDEKSYVI
                     NNYEDEKEQILSTNTTNIVKEMLRKVVSSEGTASSAKIDGYIICGKTGTAQKAVNGGY
                     LKGKYVSSFVGFLLSKKKNIAISVNVDEPMGTYYGGLVAAPIFRDILVRIINYYQIPP
                     EKDLKMVYKNETKGTN"
     gene            complement(14106..14444)
                     /locus_tag="PLF90_04600"
     CDS             complement(14106..14444)
                     /locus_tag="PLF90_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56593.1"
                     /translation="MKKNKRNLNKFRRFLFFTAIGFFLTVYVFLYLKIIYIGYETDKL
                     KEKYDFLNLLNKNYNLQIIKLTTPENLIKIAKEKNINLIVPKNWCYLEIKEENVQDTK
                     QTGILEAETR"
     gene            complement(14431..15330)
                     /gene="rsmH"
                     /locus_tag="PLF90_04605"
     CDS             complement(14431..15330)
                     /gene="rsmH"
                     /locus_tag="PLF90_04605"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOK56594.1"
                     /translation="MEKLQKKYGNEEHYPVLKEEVLKWMNLKDGGIYVDCTCGAGGHS
                     KYLLSSGKKIFLICIDKDEKMLEIAKENLKNFKNFQLIKGGFENIKEILENEKIYKVD
                     GFLFDLGFSLLQIKDGKRGFSFQIDGPLDMRYDTSSNLTAYNILNKWERKDLIYIFKN
                     YGEIKNCEKIVDKIIERRREKKFETTREFSDFICQNFKERKKIHPATRFFLALRIATN
                     NELNCLKRGLENSLFFLKNWGRILVISFNSLEDRIVKNFFREKSEINILTKKPVIASE
                     DELKKNPLSRSAKLRVGEKNEKK"
BASE COUNT         5210 a         2354 c         1950 g         5885 t
ORIGIN      
        1 ccaggtacaa aatttaaaat caggacaacc tgtttccaat gatatattaa atcaacccca
       61 tccattttta tgtcagaaat gtggattccc tttgcagacg gaagagtcca catgtccaaa
      121 ttgtttaaaa cagggagcaa tattatcaag agttttttct ttactttctg agcatattgg
      181 atggattttt attattttct ttttaatggt tataggagtt gccttaagtt taatacctcc
      241 aagaattaca aaaattcttg tagatgaagt tttaaccacc cgaaaacatg ttgactggct
      301 tccatatctt gtaattatac ttgtcagtgc tgaatttttc cgtgcccaga ttaatatgct
      361 tgtaggaact atatccactt ctgtcgggac actgattaca aataatttga gaaaaaaatt
      421 attcaaaaaa cttgaagaat tgtctttgag ttactacgac ataaattcag ttggaactat
      481 aatgacaaga ttttcttctg atgttgaagc atttcatggt tttgtaactc aggcaggaca
      541 gggtttcctc ttaaatatat ttatgttaat tggaataggt attatgcttt tttcaataaa
      601 ctggttactt gctttatatg ttatgatacc tataccattt gtagtgattg gaacccttat
      661 attctggcgt ggtgtttatc caaaatatta taaggtctgg gatagtcagg caaaactttc
      721 aacatttttg aataatgttc tttctggaat aaaaacagta aaggcatttt ctcaggaaga
      781 aaaagaattt aatagattta ctttctacgc tgataaatta aaggattcgg tcaggacagt
      841 caatataaac atttctgttt tcaatccact catggctttt ctcttcagtt taggtggttt
      901 gattatatgg tatgttggag gtaaaaatgt tcttgagggg aaattaactc ttggtgaatt
      961 aatggctttt ttgagttata ttggtatgtt ttattcacct ttaacaaatc ttaccctttt
     1021 atcaacctgg ttcagtaatt ttacaaccgc cagtcataga atttttgaag ttcttgatac
     1081 tgaaccacag gttaaagaac caaaaagggc agtgaaactt atgaaaatta aaggtgaaat
     1141 tgaatttaga aatgtaactt ttggttatga cccgtatcaa cctgttataa aaaatgtgag
     1201 ttttaaaata gaacctgggg aaatagtcgg aatagttgga aggagtggga gtgggaaaac
     1261 tactcttgtt aatttattat gtaaattcta tgatgttcaa gaaggagaaa ttttaataga
     1321 tggaaaaaat ctaaaagata taccttcata tgaattgagg aaacatatag gacttgtatt
     1381 acaggaacct tttttattta gaggaacaat ttctgaaaat atatcctatg gaaagccaga
     1441 tgctactttt gaggaaatta tcaatgcagc gaaagttgcc aatgctcatg aattcattat
     1501 gaaatttcca aacggttatg atactttact tggagagaga ggtgcaggac tttctggtgg
     1561 ggaaaaacag agaataacaa ttgcaagggc agtcttatgt gaccctccta ttctgattct
     1621 tgatgaagca acatcttctg ttgatacaga atcggagaaa aaaattcagg atgctttatc
     1681 ttcactctgg aagggcagaa ctactattat tattgctcat cgtctctcaa ctttaaaaga
     1741 atctgataaa attattgtta tagataatgg aagaattgca gaaattggaa atcataatga
     1801 attaatggaa aaaagaggta tatactataa tcttattgtt atgcagacac aactttcaag
     1861 tattgatgga gcattgatac atggaaatta aaaaagaata tttgaccccg gataatatta
     1921 aaattttaaa aggagaattc aatacaatta atcttttttt aatagaagat ggaattcttt
     1981 ataagagtgt ttttgcagtt tcatgtttcc ctgttaccaa tccctataat ttcatttcaa
     2041 ttttctatca gaaagaaacg ggtgaaatag aagaaatagg aataattaag aatatttctg
     2101 tttttcctga agaggaaaaa aagttgataa tagaaacact taataaacat tatttttatt
     2161 atgaaataac cagaatttta gatattaaat ggaaatttgg atttcttctt tttaaagtag
     2221 agacagataa aggcgagaaa gagttttact tgagatggga aaggagtaag gcaattgaca
     2281 ttggtaagaa gggtaaactt ttaatagata tttttgagga tagatatatt atatctgatg
     2341 ttgagaaact tacacctgtt gaaaaaaatc tttttacaaa gtttatttac tggtaatata
     2401 aaaactaaaa aggagaaagt atgcgaactg ttgctatttc tatggcatat gaatacagaa
     2461 aagtaagaaa gattgaagat aatttatctt atattgagaa tatttttaag gatataaaaa
     2521 atataaatcc tgaccttgtt gtcttacctg aggtttttgc atatgttggt attgaatttg
     2581 aaaaaagaga ttttggaaaa tgggaaaaga taaaagagtt tttaaaatat attgcgagaa
     2641 agacaaattg ctgggtttgt gggtcaagtt atgatttagt tgatgaaaaa atttataata
     2701 gttgtttttt tgtaaacagg aaaggagata tagtaggaaa atatgataaa attcatccaa
     2761 cagaaggaga aatagagaaa ggtatttatt ctggaagaaa ggatcagaaa ccaattgaaa
     2821 cagaatttgg taaaattggt gcatttattt gttttgatgc aaactggcat tatgacttaa
     2881 aatatcaggt gaataatgga gcaaaattac ttgttttttc ttcagcatat cctgcaggta
     2941 aaatattaaa ttcaatatct cttttatatc aggtttttat agttgctggt atatggtctt
     3001 taaattctgg aataatagat aatactggaa gatggttggt aaaaacagac agattttcat
     3061 actgggtatg gtcagatata aatcttgaaa gaactgtttt tcactgggat taccagggag
     3121 ataaagtaag aaaaataatt gaaaaatata aggatggagt gaaactagaa acttttgggg
     3181 atgaagcgat ttttacaata gaagttgttg atagaaaaat atcaatagaa aaaataataa
     3241 aagaatttaa acttgtaacc tataaagaat atttaaagag agcagaaatt atgcagaata
     3301 aaaaaagata aaatttaaat ctgacaaaat gataaaatta cttacgatag atcttgatgg
     3361 gactctactt aacaataaag gagaaattaa taaagaagat aaaataacct taaaaaaatt
     3421 ttataaatcc ggtggcattg ttgttcttgc ttcaggaaga atgactgatt gtgtttctgt
     3481 atattcagaa attcttggaa ttgatactcc tcttattgtt tataatggtg caatggtcag
     3541 attaacagaa aaagaaaaaa gaaaaataat ttatcataat cctgttcctt taaagtactc
     3601 aaagtttata attaattatt gtttggagaa caaattcctt cttaatttct actataatga
     3661 tactctttat gctgtgaatg acccattgtt aaaaaaatat gttgatattt actcaaaaca
     3721 gacaggtgca aaatattttt ttgttgatag tttagaaaat tttaaaaata aaaaaccaac
     3781 aaaacttatt ttaataacag atactgaaaa tgagaatatt aaaaggacga gagatttcca
     3841 gtacagatat ttcaaaaaac tttttggtaa aagtttgaat attgtaaaaa caaatccaga
     3901 atatcttgaa ttcatgaata aaggggtgaa taaaggaatt gctttaaaag cacttgccaa
     3961 atattacaat ataaaacccg atgaaataat tgcttttgga gatggagaaa atgatattga
     4021 aatgctttta tatgcaagaa tttcagtcgt tcccgtaaat gcaaaagaga aggttaaaaa
     4081 aattgcaaag attgtttctg attattcaaa tgaagatgcg tatatctcaa aatttttaaa
     4141 aggattttta tctatatagt tttatttttt gtttttaatt aaatttttta aagaacggat
     4201 tgcctgtttt attctgtgtt cattttcaac aagtgctaat cttacatatc cttctccata
     4261 ttttccaaag ccagcacctg gagaaactgc aacatgggca ttttcaagga gatataagga
     4321 aaatttcatt gaacccattt ttttaaattt ttcaggaatc tctgcccata tatataaagt
     4381 tgcttttggt ttttcaacat tccatccaat tctattcagt ccttcaacca taacatctct
     4441 tctttttcta tatgtctccc tcacttcatt tatatatttt tcttccaatc ttaaggcaca
     4501 tattgcagca acctgaattg gagtaaaaat cccataatca taataacttt ttaatttagt
     4561 caatgctctt atcacatatt tatttccaac ggcaaaacca accctccaac cagccatatt
     4621 atatgttttt gaaagagaat aaaattctat cccaatactt tttgctccat ttacttcaag
     4681 aaaactgtgt ggtctatcct tatcctcaaa atatatttct gagtaagcaa tatcatgtat
     4741 aataataagg tcttttttct ttgcaaattt cacaacatct ttaaaaaaat caatatcaac
     4801 aatttgtgtt gtgggattat taggataact taaaatcatt gcttttggtt taggaaatct
     4861 gtcataaatt ggttgattga gatcaggaat aaaattgttc tcttttttta gtggtaaatc
     4921 atatattgtc gctcctgaaa taattgcact gtaaagatgg cttggataag taggatttgg
     4981 tataagaaca acatcccctt tatcaaaaat tgcaagagca agatggctta tcccttcctt
     5041 tgaaccgata cagacaacta cttcttcttc aggatcaagt ttaactccaa atctttttaa
     5101 gtaccagttc gctatttcct ttcttaagtg aaaaattcct tttgaagcac tgtatctgtg
     5161 aacctttgga tctttcaaaa cttcttttaa tttttctatc acaggtgcgg gtggtggttt
     5221 atcaggattt cccataccaa aatcaataat atcttttcct tctcttctta attgtaattt
     5281 ttttgcattt attgcttgaa acatataaat tggtaaattt cttattcttt ccgattctat
     5341 atttttaact ttcatttttc tcctcaaaaa tttttatcac cttttttaca ttatttaaac
     5401 ttaatttgtg tatgattata tcagcaattt taattttttc aagtggttga gtagaaggaa
     5461 gagcaacaca gaaaattcca cttgattttg ccgagataat tcctgtttca gaatcctcaa
     5521 aagcaattat attttttgca ggtattttca attttctgga aacctcggta tatatatcag
     5581 gaaatggttt tggtttctta acatcatttc ttgtaattac aaggtcaaaa aatttatata
     5641 tatctgaaat tttaagtacc tttaaaacaa attttttttc agaatttgaa gcaagtgcaa
     5701 ttttgtattt gttttttaaa aatttaagta ttttttttgt ttcttcagaa actcctaaat
     5761 tctttttgtt cacaatttta agcaactgct tatttttttc tgaaaccaat tcttctattt
     5821 taaaaatttt tttaattttc cctttctttt ttaaattttt taaaaaaacc ctgtcctcaa
     5881 caccaactcc ttcagcaaag tcgtcttctg tcagttcaat gttatgaaat ttgaagatgg
     5941 ttttccatgc aattacatgc aatttttctg tatcaaataa aacaccatca ccatcaaaaa
     6001 caattccttt caagttcatt ttaattttaa aattgtttct tctatatcct ttgaatggtc
     6061 ctgatttatt atattttcag aaatttttag tagaattcca agtgagatta aatttgctaa
     6121 aagagaagaa cctcccacac tgaagaaggg aagagttgtt cctttttgtg gtagaataga
     6181 tagaacaaca cctgcgtgta ataaaaactg agtcccaaaa gaagtgctta ttccaaatgc
     6241 taaaatcttt tcaaataaat cattgcagaa tttggaaact tcaagtccac aaaaaactat
     6301 aatactaaaa agaagaagaa ttccaaaaga accggcaaaa ccgagttctt ctcctacaac
     6361 agcataaaca aaatccttat gaatttctgg aacaaatttc agttttcctg ttcccgcacc
     6421 aagtcctttt ccaaataaac ctcctgaact taatgatatt tttgcctgtt tgggtggaaa
     6481 gtttttgcca agcgggtctt catttttatt cagatataac ttaattcttt ctattctata
     6541 aggtgaaaga agaattagtc caagcattaa aattataaaa aatccagaga tagaaaaaat
     6601 atattttgat ttaagaccac ataaaaaaag aactcccagg aaagtaacag agataattaa
     6661 aaatgttcca tagtcctttt gtaattgtaa aattgcgata ataaaaagat aaatgatgat
     6721 gggtattatg agtgtcttta aattatttac catagaaatt cttttcttta aataaatagc
     6781 cagatatata atgaaagtaa tttttacaaa ttcagatggc tgaaaagaaa aaggtccaat
     6841 ttttatccat ctcagtcctc cctgaatatt tggtaaaggc aaaattatca gagaaattcc
     6901 caaaatcata agtccatatt tttccagttt ttttatatcc gatttctgaa ttagataaaa
     6961 tataaataag ccagcaaata gataaattaa atgtttttca aaatataata ttaaattatt
     7021 tttttccaat ccataaaaaa aacttgaact taaaacaaaa acagttccga gaagaataag
     7081 tgaaaaagtt gaaacaaaaa tactaacata aagaaaatac ttcttttttg aaaacatcgc
     7141 ctctctcctc gtaatttttg aacatatcaa aagaagagca accgggagaa aaaaggacaa
     7201 aatctccttt tttcccaacc ttttttgata gtataactgc ttcttttaaa tttccaacat
     7261 attccattgg tatatttgat gatgcaagtt ctttttttat cctttctttt gcttctccaa
     7321 gaagaattat aaacttaacc ctatttttaa gatattttgt aagttgatgg tatggaaatc
     7381 ctttatcttt tccaccaagt atcaaaatta tattattttt accttttaaa cttaatacag
     7441 aatttttaac agaatgggga tttgttgatt tagagtcatt tatatagata acaccatcaa
     7501 tttcagcaac tttttccatt ctgtgaggaa gaccttcaaa attttcaatt gcagttttta
     7561 ttgtttcctg ttcaattccg catagaatag aaatcaaaga agaagccatt atattggcaa
     7621 tatttccttt cccctgaatt tttaaatttt tcgtttcaag gatttttaaa tttttgttat
     7681 ccagattcag aaatatattt tcgttttcat aataaacacc atttgaaatt tttgaaaaac
     7741 taaaaaatct tttttgactt cttatgtctt tcaataatct ttttgtatga taatcatcaa
     7801 aatttaacac actgaaatca ttttcttttt gattttcaaa aattcttttt tttgcctgta
     7861 tatattcttc catgtttaaa tatctgtcaa aatggtcata atcaatattt aaaaggcagg
     7921 atatataagg tctgaaattt actatttttt ctaactgaaa actgctgact tccagaataa
     7981 tccacgtttt ttcttctatg tttttaatct ctcctataaa tgtatttcct atattaccgc
     8041 atacaaccga tggataattt gcttttttaa aaatatttcc aataagggtg gtcgtggtcg
     8101 tttttccatt tgttcctgtt attgcaatta tttttttgct ttttgaaaaa ttataagcaa
     8161 gttctatttc acttataaac tggatttttc ttttttttaa aaaatctata aactcatttt
     8221 tttcatccac tcctggactt attacaacaa actcaacttc tctaaaaaat tcaggggtat
     8281 gttctcctgt ttcaattttt gctccttttt cttttaaaat attaaccttt tccagtatgt
     8341 cttcattaac atttttttca gaaataaaaa cctctcctcc gttttcaagt aaaaaaatac
     8401 ctgtatcaaa tccactaatt ccaagtccta taattcctat ttttttccct tttatctggt
     8461 tcatcttatt ttcagggtta taattgtaaa taatgcaagt attattccta ctatccagaa
     8521 tcttattaca acttttgatt ctgaaactcc tttaagttga aagtgatgat gaataggggt
     8581 cataagaaaa attctttttc ctctactttt ataagaaatg acttgaatta taacagacaa
     8641 tgcttcaact aaaaatatac caccgacaaa tattaatgaa agttcctgtt taactattat
     8701 agcaagaagt ccaacaattc caccaagtgg taaagaacca atatctccca taaatatttc
     8761 agcaggataa cagttaaacc acagaaatcc aagacatgaa cctacaagtg cacctgaaaa
     8821 aactgttgct tcttctgcac ctttaacaaa gggtacattt aaataatttg caaattttac
     8881 attacttact acataggaga ttattgcata agccagagat acagttaaaa tgagacctat
     8941 tgcaagtcca tccattccat ctgtgagatt gacagcattt gatgtggcaa ctattatgaa
     9001 aacagcaaaa aggatgaaat attctccaag aggcaaaatt gcttttttaa aaaaagggat
     9061 atacaaagag gtgttaaaat ttaaatcgct cttatataca agaataagtc caataataaa
     9121 accaactatc atctgtacta taattttaaa aagtggtctt actcctttgt gtgatttata
     9181 tcttaatttt gtatagtcgt ctataaaacc aacaaatcca agagaaaaag ttaaaaaaat
     9241 gagaagtata atgtaagtat ttttaagatc tgcccagaaa aatgtagata ttaacaaagt
     9301 cccaagaatt aaaaatcctc ccattgtagg agttttttct ttttctgttt ctcttaaata
     9361 atttggatga cagaaatttt ttaacttatt tatgattttt cttccaaaca aaagagaaag
     9421 taaaagagat gtgaatgttg caagagttgc tcttactgtg acatatttaa aaatattaaa
     9481 accaaaccat aaatctgtaa aagaatataa aagtttatat aacatttaag atacctccgt
     9541 taaaaaaaat ctgtttttaa aaaatcaact atttcatcca ttttaacaat tcttgaacct
     9601 tttataaaca cagcatcttg tttttttatt tcccttttaa gttctttttt caattcatca
     9661 atttcataaa aatgttttcc ttttccagaa acttctgaaa tttttttact ttcttttcca
     9721 aaggtaaaaa taatatcaat ttttaaattt ctaatcaaat ttcctatata ccagtgataa
     9781 aagtttgaaa attttccgag ttctgccata tctcctataa ttgctatttt tcttttaaat
     9841 ttttttcttt caaatgacaa caaagaattt tttaaagaat ttggatttga attataactc
     9901 tcatcaatta taaaaatatc atcctttata aattctcccc ttcctttgag tggtttaagt
     9961 ccgtataaaa cattttttat atgtttttct gatattccaa attttttccc aaaagcgata
    10021 ccaaaaagcc cagaataaat gaaagaagta ttccagaaat tcattttata tttttctcca
    10081 gagtattcaa atatgaaata atcaaattcc tcttcaataa tttctcctgt tatatctgca
    10141 tttttcttta caccaattgt aattaaattt tttgtatctg tttttccctt taaataataa
    10201 aaaaaatcac tatctttatt aagaaaaaca tttttttcct taataagatt accaaaaatt
    10261 tccgattttg catctgccag ttcttttcta tttttaaaaa aaccaatatg agccactcca
    10321 atatttgtta taacacagga atttggtgca gatattttac ttaaataatc aatttctccc
    10381 tttttattca ttccaatttc aaaaacacca atttctgttt ttttatctgc ctgtaaaata
    10441 gaaaggggaa gaccaatttg attattaaaa tttccaattg ttcctgttgt attaaacttt
    10501 tctgataata tttcttttat aatttctttt gtagttgttt taccatcact cccagtaata
    10561 ttaataacat gcatatttaa tttaattcta taatttttac ctatatctcc aagtgcgtat
    10621 agagtatctt ttacttttat cagtaaatta tcgttcccta tgtaatcact ggatacaatt
    10681 gaagctactg ctcctttttt aaatgcatct tttacaaaat catgaccatc atatttttcg
    10741 ccttttaaag caataaaaat ttctcctttt ttaatctgtc ttgaatcagt tgaaattcct
    10801 tctataaaaa aatctttaaa cttcttgtta acctttcctt tacaccactt tataatttca
    10861 ctcaatttaa ctttttccat tttaaagtaa tttttcttcc ttttttctat taattatttc
    10921 ttttattgct tttcttgctt cttctctatc gtcaaaaggg attgtgacat cttttaatat
    10981 ttgaaatgct tcatgtcctt tacccgctat aacaacacag tcattacttt ttgccattga
    11041 aatgccttcg tatattgcct tttttctatc agggattgca acatatttct tcctgagata
    11101 aaatggaata cctctttcaa tatcttttat tattttaatt gggtcttctg ttcttggatt
    11161 atcagatgtg ataataacaa aatcagccat ttttactgca atttttccca ttaaaggtct
    11221 tttactttta tctctatctc ctccacatcc aaaaatgagt attattcttt ttggtcttaa
    11281 acttttgaca gaaagtaata aattgttcag tgcatgatga gtatgtgcat agtcaacaat
    11341 aactgaaaaa tcctgtcctt catcaataaa ctcaaatctt ccaggtacat tttcaatctt
    11401 taaaaatgct ttttgaactg tttcaaaagg aattttataa atataagaaa atgcaattcc
    11461 tgcaagtaaa ttatatacat tccccagtcc actaatttta ctattgaaaa ataatttttc
    11521 attttcaatt gcaatttcta aatagtttcc tcctttttct actctataac tcaaaagttt
    11581 tacatttgag ttcctgtttt taccaaatgt tatccattta ataccatttt tttcacatcc
    11641 cttgatgaaa actttcgcat agggagaatc taaattaatt attccgtatt tttccttttt
    11701 ttcactgtct tttaaatatc tcgtaaataa aagaagtttt gcatttaaat aatttttaaa
    11761 tgttttatga taatcaagat gttcatggga agcaatgttt gtaaagattc cagcattaaa
    11821 gtcaatacct tctatccttc tctgttcact tccatgagaa gaaacttcca taacagccca
    11881 cttacaacca ttttttaaca tttcagaaaa catttcaatt aaatcaagtg attcaggagt
    11941 tgtatttgtt gatacaattt ttctctctcc tatttcataa aaaattgttc ctataagacc
    12001 tgccttaatt ccattttcat ttaatatttc ttttattata aaacttgttg ttgtttttcc
    12061 atttgttcct gttattccta caatatttaa tttttttgaa gggaaatcat aataaatatt
    12121 tgcaatctca ctcaatgttt ttttagtatc ttcaacaaca atttctgtaa tatctgcagg
    12181 tataattgaa ggtcttttgc ttacgattac acattttgct cctctttcaa tggcttcatt
    12241 tataaagttg tggccatcct gttttgttcc ttttattgca ataaaaacat aattttcttt
    12301 aatttttctt gaatcataag caattccctc aacttcaaaa ttattaagat taaaaacctt
    12361 ttttttcttg atatctttaa ttagttcctt tagtttcatt tttatatacc atttttaaat
    12421 ctttttcagg aggtatttga taataattta ttattcttac aagtatatcc ctgaatattg
    12481 gagcagcaac aagccctcca taataagttc ccattggttc atctacatta actgatattg
    12541 ctatattttt tttcttactc aataaaaatc ctacaaaaga agaaacatat tttcctttta
    12601 aataacctcc attcactgct ttttgagcag ttcctgtctt cccacatatg atatatccat
    12661 caattttggc agaggaggca gttccttctg aactaacaac ttttcttaac atttctttta
    12721 caatatttgt tgtatttgtt gataaaatct gttctttttc atcctcataa ttattaatta
    12781 cataactttt ttcgtctttg atttctttta ttatatgagg tttaacaaga tatccaccat
    12841 ttgcaaatac agaaattgct cttattccct gaattgaagt tattccaacc tcctgtccta
    12901 taggtatggc tgtaattgaa tatgaagacc agttttcaag tggtctgaat atacctccta
    12961 tttctcccgg taagtcaatt cctgtccttt ccccaaattt aaattttttg caatatttat
    13021 agagtttttc ttctccaaga gacattgcaa ttttaacaat cccaatatta cttgatttaa
    13081 caactgtttc ttcaaatgtt aaaattccat aagggtgaac atcatgaaga taatgatctc
    13141 ttacaaacca tttcccatat tcgcacatta ttgttgaatc tggagtaaaa atattttcct
    13201 ctatagcagc ggcagcagta acaattttga ataccgaacc tggttcaaaa aggtcggtaa
    13261 cacatttatt tcttataatt gataaatcaa attttgaaaa attgtttggg tcataatcag
    13321 gataatttgc aagagcaaga atttctcctg tgtcagcatc cattacaata acagaaatat
    13381 tttttggact gaatttttca tatccatttt taatttcctc ttcaacaata aattgaatat
    13441 ctgaatcaat tgttaagaca atgtcttttc ccttttcttc ttttataaga gtttttttaa
    13501 tagaaggaat taaatttccc agtccatcct ttaatacaag atatatccca tcttttcctt
    13561 ttaaaaatga atcataaaaa tattcaactc cttcaagtcc ttttccatca atatctgtat
    13621 agccaagtat atgacaggcg tgtcttccat aaggataaac tctcttatat tttctttcaa
    13681 agtatatacc tttaagattt gtttttttta ttttttcata ttcttcaatt tcaatatctt
    13741 ttttgattaa agcatatggt tcattcatct tttcttctat ctgtttttta tctattttaa
    13801 gaatttcgga cagtttattt ttcaattcgt ttaaatattc tggatttttt tcttttaatt
    13861 tttgaatcat ccatgtatca aaatataaag aataatttgg taaattaaaa gcaagaagat
    13921 ttccccatct atcataaatt ttacctcttt ctgcaagaac ttttattttt gcatatctag
    13981 ttttcctttc aattatttta tattttgagt acattattac ctgaagatta aaaagttgac
    14041 agaaaattaa cagaaaaccc gtaaaaaaaa taagttttgt atatctaatt cttctttcaa
    14101 aaatatcatc ttgtttctgc ttcaagaatc cccgtttgtt tagtatcctg tacattttct
    14161 tcctttattt ctaaatagca ccagtttttt ggaactataa gattaatgtt tttttctttt
    14221 gcaattttta ttaaattttc aggtgttgtt agttttataa tttgtagatt ataattctta
    14281 tttaaaagat ttaaaaaatc atatttttct tttaatttgt ctgtttcata gccaatatat
    14341 attatcttca agtataaaaa aacatagaca gttagaaaaa aaccaatggc tgtgaaaaat
    14401 aaaaaccttc taaatttatt taaatttctc ttattttttt tcattttttt ctccaactct
    14461 cagttttgca cttctactta aaggattttt ttttaactca tcttcagatg ctataactgg
    14521 tttttttgtt aagatgttta tttcactttt ttctctgaaa aaatttttta caattctatc
    14581 ttcaagagaa ttaaaactta ttacaagaat tcttccccaa ttttttaaaa aaaataatga
    14641 attctcaagt cctcttttca aacagtttaa ttcattattt gttgcaatcc ttaaagcaag
    14701 aaaaaatctt gtggctggat gtattttttt cctttcttta aaattctgac atataaaatc
    14761 agaaaattct cttgttgttt caaatttttt ttcccttctt ctttcaataa ttttatcaac
    14821 tattttttca cagtttttta tttccccata atttttgaat atataaatta aatcttttct
    14881 ttcccattta tttaaaatat tataagcagt taaattggaa gatgtatcat atctcatatc
    14941 aaggggtcca tctatttgaa aactgaaacc tctttttcca tctttaattt gcaacagaga
    15001 aaaaccaagg tcaaagagaa atccatcaac tttataaatt ttttcatttt ctaaaatttc
    15061 ttttatattt tcaaaaccac cttttataag ttgaaaattc ttaaaatttt ttaaattttc
    15121 ttttgctatt tcaagcattt tttcgtcctt gtcaatacaa attaaaaaaa tctttttacc
    15181 tgaagaaagt aaatatttac tgtgtccacc cgctccacat gtacaatcta cataaattcc
    15241 tccgtctttt aaattcatcc attttaaaac ttcctctttt aaaacaggat aatgttcttc
    15301 attcccatat tttttctgca atttctccat aagaaggacc gtatttttcc cagaattctt
    15361 tccagtttaa ctcatcccag atttctatcc ttttacctg
//
LOCUS       DAOSAT010000039        15315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2648_length_15315_cov_5.759174, whole genome shotgun sequence.
ACCESSION   DAOSAT010000039 DAOSAT010000000
VERSION     DAOSAT010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15315
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2648_length_15315_cov_5.759174"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2146)
                     /gene="bamA"
                     /locus_tag="PLF90_04610"
     CDS             complement(<1..2146)
                     /gene="bamA"
                     /locus_tag="PLF90_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOK56595.1"
                     /translation="MKPKKILSVFLILSFYVFSIPILKINITGNKNVSVDKILTLMKT
                     KEGIEFDERVIKDDISNLIKTGYFKDVKYTLEKKETGIELNLEVKEKPVIEKIIFEGN
                     RALKTKKLKEISELKEGDFFNEEKVVEGIEKIRNQYYKMGYHASEIDYEVEEKDGLCI
                     LKIQINEKGKGYVSEILFEGNNIFPDKRLKKLMKIKTRKMPFIRGTYKEDILEEDIKR
                     IKDFYNENGFIEVKIEKEIEYKEKGIIIKIFVEEGKRYFVGEIKFEGELIFNEDILRN
                     QVLLKRGDVFNVKKNEESLRNIYKIYSDKGYIKCNVEFIPEIRGDAVNITYLINPGAI
                     YYTEEVKIKGNKITKDKVIRREIKLEPGDKITGDKIRKSFNNLRDTNYFENIRIYPEF
                     VEEGKANVVVDVKEREKTGLFLIGGGYSSIEKFVGMVSIQQTNFDITNPPKFIGGGQK
                     ISLSFEIGTVAKNYMFSFTEPYLFDRPIYIGPDIYRLERNWDDWDTTSTGFDLRIGRK
                     WENFNLGFKLLSENVNLYDIQIPSIISQEGKKRKNSITTYLTYSNLDSDMFPTKGDKV
                     RLSLEYAGLGGDIDFTKTTLENNFYYPLKNFIFHSKTMVGYINKDYDEIPIYERFFGG
                     GIGTVRGYVERSLGPKEIDSEGVVHYLGGKFIFAQNFEMMYPLYKDVLYGIGFFDIGN
                     VDKDWSFSNLKKGIGAGIRVNIPFFNAPVEIYY"
     gene            complement(2106..4634)
                     /locus_tag="PLF90_04615"
     CDS             complement(2106..4634)
                     /locus_tag="PLF90_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOK56596.1"
                     /translation="MFERFTKRVKIILDIARKEALKYGHNKVEPEHILLAMLQEGEGV
                     GIAILNYLGVETERLKYELEKVIKRGVPFYSGELPLSTSSKRVLEYSVREAQFFGHSY
                     IGSEHLLIGILLESESLPAQILRKFGITVDKARNILEEILRDLELPGNTPYSIEEKED
                     FYYKNMPNKTSTTYKKQKTPALDTFGRDLTKLAIEGKLDPVIGRENEIERVIQILCRR
                     KKNNPVLLGEPGVGKTAIVEGLAQNIAFGKIPEILKDKRIVMIDLPSMVAGTKYRGEF
                     EQRVKVVLDEIRNSGNIIIFIDEIHTLVGAGGAEGAIDASNILKPVLSRGEIQCIGAT
                     TLDEYRKYIERDGALERRFQPVIVNPPTVEETIEILKGLKEKYESHHKLKISNEAIEA
                     AAKLSDRFIADRFLPDKAIDLIDEAASQKRLKLYKEPKEITALQEEIELLGKEKEKAV
                     KTQDYELLTSIRDKERELKNKIDFLRENFMNLISEEEKTLTKENIAEIVSQWTGIPVS
                     QLCKEEKEKLLEMEKFLHKIVVGQDEAIETISKAIRRSRAGLKDIRKPIGSFLFLGPT
                     GVGKTLLARALAEFLFGDDDALIQIDMSEYMEKFSISRLIGAPPGYVGYEEGGQLTEK
                     VRRRPYSVILFDEIEKAHPDVFNLLLQILEDGRLTDSFGRSVSFKNTVIIMTSNIGTE
                     ILKNKSVIGFLTPEEKLSFENLKCEILEKVKKTFRPEFLNRLDDIIIFHPLTQEHLLK
                     IVEIEIEKVRERLNELKVKLIVSDEAKKFLVEKGTNTEFGARPLKRTISRYVEDPLSE
                     EILKGNFKEGDEIIVERKGLFLVFIKKEGEPIETKKNPVSISNS"
     gene            complement(4627..5670)
                     /locus_tag="PLF90_04620"
     CDS             complement(4627..5670)
                     /locus_tag="PLF90_04620"
                     /EC_number="2.7.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HOK56597.1"
                     /translation="MEEISKFLSWDIIEKETDWVYSKLIDDVVITSRVRIARNIKGYP
                     FPYNMTVNTGKKVEEILLKVFLNFPSEKIFIINISSLNEKQKKSLIEKHLISKEFSEN
                     GLCDKIVIIPDKKASIMINEEDHLRIQVILPGLNLKKCLKLINEIDDYIDIKIEYAFS
                     PKFGYLTACPTNTGTALRSSALIHIPGIRFFKKDKTIFKNIEGIGIAVRGFYGEGSLP
                     FGSIFQMSSTESTGKTEKEIVGELESVVKLIEEEEKKCIEEIKNDGNLKKKILKKIMK
                     ILKEERENGEFIDFYSLLFLSYVSGILNFEKHELKDFFFFILENTGKNKNYIFSEKKG
                     KILKRKLWEKLNV"
     gene            complement(5654..6040)
                     /locus_tag="PLF90_04625"
     CDS             complement(5654..6040)
                     /locus_tag="PLF90_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56598.1"
                     /translation="MDKCFICKSRSYLSIKFKGKKAKKIEFNFCKKCWEEYNSFFNNN
                     KRRKYDKCPVCGYTIDEFKEYRFLGCDFCYEYFFKEVSDYMRKIHTNTIYKGKYPKNF
                     KKEEKIKKIYLLKGLINEITINGRNK"
     gene            complement(6033..6911)
                     /gene="ilvE"
                     /locus_tag="PLF90_04630"
     CDS             complement(6033..6911)
                     /gene="ilvE"
                     /locus_tag="PLF90_04630"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOK56599.1"
                     /translation="MGLIIYLDGKFVDENEAKISIFDHGLLYGDGVFEGLRSYNGKIF
                     KLDEHIIRLYNSAKAILLEIPVEKEELKKIIIETVKRNNLKDSYIRVVVTRGVGDLGL
                     DPRKCKKPTIFVIASKIQLYPESLYDKGIDLVTVATRRNLNESLNPAIKSLNYLNNIL
                     AKIEANNSGASEGLILNQFGYVSECTGENIFIIKEKNLITPPVSAGALDGITRRVVME
                     IGENIGLKVKEENITRYDIYTSDECFLTGTAAEIVPVVVVDNRIIGDGKPGKITMKIR
                     KEFHLLTEIEGIPVYG"
     gene            complement(6902..7591)
                     /locus_tag="PLF90_04635"
     CDS             complement(6902..7591)
                     /locus_tag="PLF90_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK56600.1"
                     /translation="MNKIKGEKLTKNYNGVIAIKDVDIEIKKGEIVVIIGPSGSGKTT
                     LLNLLGLIDKPTEGKVYIDEKDTETLTEKEIAEIRNKKFGFIFQFFYLIPELKIIENV
                     FLPLWIREKRFSPEFLKEAEKYLNVFGILDKKNTYPSRLSGGELQRVAICRSLICQPE
                     IIFADEPTGSIDNKSANSFFEFMKKFNEEKETTFVIATHNEKFLQFASKVVYLNEGKI
                     ERMEKKEMKWD"
     gene            complement(7584..8765)
                     /locus_tag="PLF90_04640"
     CDS             complement(7584..8765)
                     /locus_tag="PLF90_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK56601.1"
                     /translation="MISYNFFLAKKFIGNKREDRFLWFISFFTVLGISIGVVTLLLVI
                     GVMNGFSNDLKRKIIGANPLITIEGKPYIYNYEEIIEKVKNLPEIKGVSPYIMSQVIY
                     KSNRYILGGIIKGIDLKRETDVTNIKNFIKKGSIEEIENGIILGKELAGELGVEVGDY
                     IFIIAGLIPKERIFKVVGIVEYGVYSFDVSMGFTSLKNLMEIFNMSGVHGIGVKVDDI
                     YKTPVISDKIRKLIDENYSISTWIEKNKILFSAIALEKKAMAIILSLIVIVASFNILS
                     TLMISVYRKVKEIGILKAMGLTSGEIKKIFLYQGIYLGIKGLIFGMIFGFLLSWILKK
                     YNFINLPEFVYDISKLPIEISLFDTIWIFFLVIIVSCIASIYPAQRASKLNPAEAIRN
                     E"
     gene            complement(8762..10651)
                     /locus_tag="PLF90_04645"
     CDS             complement(8762..10651)
                     /locus_tag="PLF90_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOK56602.1"
                     /translation="MLNEYRHMMFDYFIKRTRKVYYERKKRLKDIKNRKDAEKYQEYV
                     REKIKKAFSPFPLSEKTPLNPKITGIIERNGYRIEKIIFESMPSCFVTANLYIPEKFN
                     PPFPAVVAPCGHSIEGKAEPKYQEFCQRLVHNGFLVLIYDPFDQGERNQYYFLPEKLP
                     VKKGNCLAHNMIGKQMNLIGEFFGSYRVWDGIRAIDYITSRDEWDKNFLGITGNSGGG
                     TLTTWLWAIDERLKTAAPGCFVTPLLYNLENELPQDSEQYPPGILGEGVEISDFFISR
                     VPSPLILLGQKYDYFDIRGFKEICEELEYFYNIFDKKENFTYFIGDNTHGYHSDSQNA
                     MVSFFCKIVGKNVLNVEPDIIIEKEKTLFATKKGNVLLEGSKPHFEIIREKTEIIIKN
                     RKNFDESNLKNELKKILSIPESISIPHYRVLRPVSIKNESIGRYAVETEENIWVILKR
                     RNVKKPYYLEVEEEINLYVPDISSEDEIKRFPDGKPSDYFVDVRGIGESMPDIKKYFF
                     HPYRFDYMFDGFYLLFGESYLGKRIYDVLSVINLLNYLGCKRINLYGNRQGAIISLFV
                     CLFSDLIERINLNKLPDSFYSLIKKPYTKLPSSNFPKGILKITDIPEILKIVEKKSEI
                     KILNK"
     gene            complement(10654..11403)
                     /gene="tpiA"
                     /locus_tag="PLF90_04650"
     CDS             complement(10654..11403)
                     /gene="tpiA"
                     /locus_tag="PLF90_04650"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOK56603.1"
                     /translation="MKKSLIAGNWKMYKTPAESINFVSELVKNIKSYEDRDVLICPPF
                     TSLYPVSKIIKNSAVKLGAQNVYFENEGAYTGEISPLMLKDTGVEYVICGHSERRNIF
                     GESDEIINKKVKKVVETGMNAILCVGEKLEEREKGETFNVIENQVKRCLEGFDKFENL
                     VIAYEPVWAIGTGKTALPSQAEEVHIFIRELIAKIYNKDIAENLIILYGGSVKPENID
                     QLMKEKNIDGVLVGGACLKVESFLRIIDYRG"
     gene            complement(11450..12634)
                     /locus_tag="PLF90_04655"
     CDS             complement(11450..12634)
                     /locus_tag="PLF90_04655"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014933404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOK56604.1"
                     /translation="MKIKSIKNRNYKGKKVFLRVDYNVPIEKGVITDDTRITATIPTI
                     NYLLSQGASLILASHLGRPKGKPSPEFSLKPVAERLSKILNKEVKFAPDCIGEEVKKM
                     VNELKEGEILLLENLRFHSEEENGDENFAKELASLADAYVNDAFGTCHREHASVYQIT
                     KFAKDVAAGFLIEKEVESLSKLLINPEKPFLLILGGAKVIDKINMVKNLLDKVDTIIT
                     GGVMAYTFIKAKNWEVGDSKVEEEAVPVAKEIIKEINKRHLEFHTPLDHVIVQKISED
                     AVPIITERGTVPHGWIGVDIGPQAIEEYTDCINRAKTIFWNGPMGIYEIKKFAKGTIE
                     LAKAIANSKAFSVIGGGDSAAAVDEAGVADKISHICTGGGASLEFLEKGTLPGIEVLK
                     EE"
     gene            complement(12694..14079)
                     /locus_tag="PLF90_04660"
     CDS             complement(12694..14079)
                     /locus_tag="PLF90_04660"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK56605.1"
                     /translation="MRIKFLGATKNVTGSKFLLQIGGKNLLIDCGLFQERDLKNRNWE
                     KFPVPPLEIDYILLTHAHLDHSGYLPKIVKDGFKGKILCTVPTAEITKIALLDSAKLQ
                     VEDAEKKRKRHQKEGRKGPYPEIPLYDVNDAMSVFDFFETVSYNEEVKISDNIKVVFY
                     NSGHILGSSFIKVIADGKEIVFSGDIGRKENPILSDPEILESTDILIMETTYGDKIHE
                     PREIAEEKLERIINETERKEGNIVIPTFAIERAQEILYYLKKLLLEDRIPHLFTFLDS
                     PMAIEVTEVFKKNLTYLDGEAENLLKNKISPFDFPLLHFTKSVEESKMINHIKGTVII
                     MAGSGMCTGGRIKHHLVNNIEREESSIAFVGYQAKGTLGREILEKKEVRILGNFYKVK
                     AKIEKIDGFSAHADREELLSFLSGFKKFPESIFLVHGEEEVIDEFSKVIYDRFKNSKI
                     FIPSYLDEYEI"
     gene            complement(14076..15254)
                     /locus_tag="PLF90_04665"
     CDS             complement(14076..15254)
                     /locus_tag="PLF90_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK56606.1"
                     /translation="MWKKTFISTFIAQIFSIIGFSFAMPFLPFFISELGIKDIPSQTY
                     WAGIILSASGFTFTIFSPIWGYFADKYGRKLMVVRSMFAGTFILFLMSIVRTVKQLLI
                     CRLLQGALTGTIVASVTLVASVVPVEKSGFTLGMMQTAVSIGNSVGPLIGGIISDHFG
                     YRNTFRIGGLIIFLGGLLILLFARENRINYKKKEEFSYREILKLKGFWIATMIMFGVR
                     LSNTILNPSFPLVIKEIISNPEKLNTITGTIIGIAAIIEGLSAAGLGNLSDRIGYIKM
                     IGNCFIFASIASLGHFFVHSVFSLFFWRVLFVASVAGVIPSVNSMIKTLINENSIGKA
                     YGLFTSISMLGLAFGPLIGGYTGKIINIRTPFLITSIFHAIVAISIISLIKKEKGESL
                     "
BASE COUNT         4838 a         2902 c         1643 g         5932 t
ORIGIN      
        1 cgtaatatat ttcaactgga gcgttgaaaa atggaatatt tactcttatt cccgctccta
       61 tacctttctt taaattacta aaactccagt ccttatctac attacctata tcaaaaaaac
      121 ctattccata taaaacgtct ttatataaag gatacatcat ttcaaaattt tgtgcaaaaa
      181 taaattttcc tcccagataa tgaacaactc cttctgaatc tatttctttt ggtcctaaac
      241 ttctctctac ataacctctg actgttccaa tccctcctcc aaaaaatctt tcgtaaattg
      301 gtatttcgtc ataatcttta tttatatatc caaccattgt cttactgtga aatataaaat
      361 ttttcaatgg ataataaaaa ttattttcca aggttgtttt tgtaaaatca atatctcctc
      421 caagtcctgc atattctaaa gacaacctaa ctttatctcc ttttgttggg aacatgtcac
      481 tatcaagatt agaatatgta agatatgttg taatactatt ttttctcttt ttgccctcct
      541 gagaaattat agagggtatt tgtatgtcat aaaggtttac attttcactc aataatttaa
      601 atccaagatt aaaattttcc cattttctgc caatccgcag atcaaaacca gtagaagttg
      661 tatcccagtc atcccagttt ctttccaatc tgtaaatatc aggtccgata taaatgggtc
      721 tatcaaataa atatggttct gtaaaactga acatgtaatt ttttgcaact gtacctattt
      781 caaaagataa agaaattttc tgtccgcctc caataaattt tggaggattt gttatgtcaa
      841 aattcgtctg ctgaatgctt accataccca caaatttttc tatactgcta taaccacctc
      901 caattaaaaa aagccctgtt ttctctcttt cttttacatc cacaacaaca tttgcctttc
      961 cttcttcaac aaattcagga tatatacgta tgttttcaaa ataattggta tctctcagat
     1021 tgttaaaact ttttcttatt ttatctcctg ttattttatc tcctggctca agtttaattt
     1081 ctcttcttat tactttgtct tttgtaattt tatttccttt tatttttact tcttctgtat
     1141 agtaaattgc tcctggatta atcagatatg ttatgtttac cgcgtctcct ctaatttcag
     1201 ggataaattc aacattgcat tttatgtatc ccttatctga atagatttta tagatatttc
     1261 ttaaactttc ttcatttttt ttcacattga aaacatcccc tcttttaagc aaaacctgat
     1321 ttcttaaaat atcttcatta aaaatcagtt ctccctcaaa tttaatttca cctacaaaat
     1381 atctcttacc ctcttccaca aaaatcttta taataatacc cttctcttta tattcaattt
     1441 ctttttctat tttaacttca ataaatccat tttcattata aaaatcttta attctcttaa
     1501 tatcctcttc caaaatatct tctttataag tccctcttat aaaaggcatc tttctcgtct
     1561 ttattttcat caatttcttt aatcttttat ccggaaatat attattccct tcaaataaaa
     1621 tttcacttac atatccctta cctttttcat ttatttgtat tttaagaata caaagtccat
     1681 ccttttcttc aacttcataa tcaatttcac ttgcatgata tcccatttta taatactgat
     1741 ttcttatctt ctcaatccct tcaacaactt tttcctcatt gaaaaaatcg ccttctttta
     1801 attctgaaat ttctttcaat ttttttgttt ttaatgctct attcccctca aaaataattt
     1861 tttcaataac aggtttctct ttaacctcaa gattcaattc tattcctgtc tctttttttt
     1921 ctaaagtata tttcacatcc ttaaaatatc ctgttttaat gaggttactt atatcatctt
     1981 ttataaccct ttcatcaaat tctattcctt cctttgtctt cataagagtg agtattttat
     2041 caactgaaac atttttattc cctgtaatat taatttttaa aattgggatt gaaaagacat
     2101 aaaaactaag aattagaaat actgacagga ttttttttgg tttcaattgg ctctccttcc
     2161 ttttttataa aaacaagaaa aagacctttt ctttcaacaa ttatttcatc tccttcttta
     2221 aaattcccct tcagaatttc ttctgacaga gggtcttcaa catatcttga aatggttctt
     2281 tttaaaggtc ttgccccaaa ttcagtattt gttcctttct caacaagaaa cttttttgcc
     2341 tcatcactta caataagttt aaccttcaac tcattcaatc tttctctcac tttttcaatc
     2401 tcaatttcaa caattttcaa aaggtgttcc tgtgttaatg gatgaaagat tattatatca
     2461 tcaagtcggt ttaaaaattc cggtctgaat gtttttttca ccttttccag aatttcacac
     2521 tttaaattct caaaagataa tttctcctct ggtgttaaaa atccaataac tgatttattt
     2581 ttaagtattt ctgttccaat atttgatgtc ataattataa cagtgttttt aaaactcaca
     2641 cttcttccaa aactatcagt aagccgtcca tcttcaagaa tttgaagtaa aagattgaat
     2701 acatcaggat gtgctttttc aatttcatca aaaagtataa cagaataagg tctccttctg
     2761 actttttcag ttaactgtcc accttcttca taaccaacat atccaggagg agcacctata
     2821 agtcggctta tagaaaactt ttccatatat tcagacatat ctatttgtat aagtgcatca
     2881 tcatctccaa aaagaaattc agcaagtgct ctggcaagta gagtttttcc tacaccagtt
     2941 ggaccaagaa aaagaaaact gccaattggt tttcttatat cctttaaacc tgctcttgac
     3001 ctccttattg ccttgcttat tgtttctatt gcttcatcct gtcctacaac aattttatgc
     3061 aaaaattttt ccatttcaag taatttttcc ttttcttctt tacatagttg agaaactgga
     3121 ataccagtcc actgggatac aatctcagca atattttctt tagtcaatgt tttttcctct
     3181 tcagaaatta agttcataaa attttctctc aagaaatcaa ttttattttt aagttccctt
     3241 tctttatccc ttatagaagt tagcagttca taatcctgtg tcttcactgc tttttccttt
     3301 tcttttccaa gtaattcaat ttcttcctga agcgctgtta tttcttttgg ttctttataa
     3361 agttttaatc ttttctgact tgctgcttca tctataaggt ctatcgcttt atccggtaaa
     3421 aatctatcag caataaatct atcactcaac ttagcagccg cctctattgc ttcatttgaa
     3481 atttttaatt tatggtgtga ctcatatttt tctttaagtc ctttaagaat ttctattgtc
     3541 tcttcaactg ttggtggatt aactataaca ggttgaaatc tcctttcaag tgctccgtct
     3601 ctttcaatat attttctgta ttcatcaaga gtagtggctc caatacattg aatttcacct
     3661 ctggaaagaa ccggttttaa aatatttgat gcatctattg ctccttctgc ccctccagct
     3721 ccaacaagag tatgaatttc atcaataaaa ataattatat tgcctgaatt ccttatttca
     3781 tcaagaacaa ccttaaccct ctgctcaaat tctcctctgt atttagttcc tgctaccatt
     3841 gatggcaggt caatcataac aatcctttta tctttcaata tttcagggat tttaccaaaa
     3901 gcaatattct gtgcaagtcc ttctacaata gcagttttac caacaccggg ttctccaagt
     3961 aaaacaggat tatttttctt tctcctgcag agaatctgaa taactctttc tatttcattt
     4021 tctctcccta taacagggtc aagttttcct tcaattgcaa gttttgtaag gtcccttcca
     4081 aaagtatcaa gtgcaggtgt tttctgtttt ttataagttg ttgatgtttt attgggcata
     4141 tttttataat aaaaatcttc cttttcttca attgaatatg gagtatttcc tggtaattcc
     4201 agatccctta aaatttcttc taaaatattc cttgctttat caacagttat tccaaatttc
     4261 ctcaaaatct gtgcaggtaa tgattcagat tcaagtaata tacctattaa aagatgttca
     4321 cttcctatat atgaatgacc aaaaaactgt gcttctctca cagaatattc aagaactctc
     4381 ttggatgaag tacttaaagg taattctcca ctataaaaag gaacccctct tttaataact
     4441 ttttccagtt catatttcaa tctttctgtt tcaaccccga gataattcaa aattgctatt
     4501 ccaactcctt ctccttcctg aagcattgct agtaaaatat gttctggctc aactttatta
     4561 tgtccatatt taagtgcttc ttttctcgct atatccagaa ttatctttac tctttttgta
     4621 aatctttcaa acatttaatt tctcccacaa tttccttttt aatattttac cttttttttc
     4681 agaaaaaata tagtttttgt ttttaccagt attttcaagg atgaaaaaaa agaaatcttt
     4741 taactcatgc ttttcaaaat ttaaaattcc acttacataa gagagaaaaa gaagggaata
     4801 aaaatcaata aattctccat tttctctttc ttcttttaaa attttcataa tctttttcaa
     4861 aatttttttc ttcaaatttc catcattttt tatctcttca atacattttt tttcttcctc
     4921 ttcaatcaat tttaccacac tttcaagttc tccaacaatt tccttttctg ttttacctgt
     4981 tgattccgtt gaagacatct gaaatatgct tccaaaaggc aaactgcctt ctccataaaa
     5041 tcctcttact gcaattccta ttccctcaat atttttaaaa attgttttat cttttttaaa
     5101 aaatctaatt cctggaatat gaatgagtgc agaacttctt aaagcagttc ctgtattggt
     5161 cgggcaggca gttaaataac caaattttgg cgaaaatgca tattcaattt ttatatcaat
     5221 ataatcatct atttcattta taagtttcaa acattttttt aaattaagac cgggtaaaat
     5281 aacctgaatt ctcaaatggt cttcttcatt tatcattata cttgctttct tgtcaggtat
     5341 tataacaatc ttatcgcaaa gtccattttc agaaaattcc ttgcttataa gatgtttttc
     5401 tataagagat tttttctgtt tctcgtttaa actagaaata tttataataa aaattttttc
     5461 agatggaaag tttaagaaaa cttttagtaa aatctcttca acttttttac ctgtattaac
     5521 tgtcatatta taggggaaag gatatccttt tatatttctt gcaatcctaa cccttgaagt
     5581 tatcacaaca tcatcaatta attttgaata aacccaatca gtttcttttt caattatatc
     5641 ccaagataaa aacttactta tttcttccat ttatagttat ttcatttatc aaacctttta
     5701 aaaggtagat tttttttatt ttttcctctt ttttaaaatt cttaggatat ttgcctttgt
     5761 atattgtgtt tgtatgaatt tttctcatat aatctgaaac ttctttaaag aaatattcat
     5821 aacagaaatc acaaccaaga aacctgtatt ctttaaattc atcaattgta taaccgcaga
     5881 caggacattt atcatacttt cttcttttat tattgttaaa aaacgaatta tattcctccc
     5941 agcacttctt acaaaaattg aattcaattt tttttgcttt tttccctttg aattttattg
     6001 atagataact tctactttta catataaaac atttatccat aaacaggtat tccttctatt
     6061 tctgtaagca agtggaattc ttttcttatt ttcattgtaa tttttccagg ttttccatct
     6121 cctataattc tattatcaac aaccacaaca gggactattt ctgctgctgt gccggtcaga
     6181 aaacattcat cacttgtata tatgtcatat cttgtaatat tctcttcttt aacttttaaa
     6241 ccaatatttt cacctatctc cataacaacc cttcttgtta taccatcaag tgctcctgct
     6301 gaaacaggtg gagttattaa attcttctcc ttaataataa aaatattttc acctgtacat
     6361 tcactcacat aaccaaactg atttaaaatc aatccctcac ttgcacctga attatttgct
     6421 tcaatttttg caaggatatt gttcagataa ttcagtgatt ttatagcagg atttaatgat
     6481 tcattcaaat ttcttcttgt tgcaactgtt acaaggtcaa tccctttgtc atacagtgat
     6541 tctggatata attgaatttt acttgctatt acaaatattg ttggtttttt acatttcctt
     6601 gggtcaagtc caaggtctcc aacccctctt gtaacaacaa ctcttatata actatccttc
     6661 aaattattcc tcttaacagt ttcaattatg attttcttta actcttcctt ttccactgga
     6721 atttcaagta atattgcttt tgcagagtta tagagacgaa taatatgttc atcaagttta
     6781 aaaattttac cattgtaact tctgagtcct tcaaatacac catcaccata taaaagccca
     6841 tggtcaaaaa tagaaatttt tgcttcattt tcatcaacaa atttaccatc aagatatatt
     6901 atcagtccca tttcatctcc tttttttcca tcctttcaat tttaccttca tttaaatata
     6961 ctacttttga agcaaattgt aaaaattttt cattgtgagt tgctattaca aaagttgtct
     7021 ctttttcttc attaaatttt ttcataaatt caaaaaaact attagcactt ttattgtcaa
     7081 tacttcctgt tggttcatct gcaaaaataa tttctggctg gcatatcaaa ctcctgcata
     7141 ttgctactct ttgtaattct ccacctgaca atcttgaggg atatgtattc tttttatcta
     7201 aaattccaaa aacgttcaga tatttttctg cttcctttaa aaattctgga ctaaaccttt
     7261 tctctctaat ccataggggt aaaaaaacat tctcaataat tttcaattca ggtattaaat
     7321 agaaaaattg aaaaataaat ccaaatttct tatttcttat ctcagcaatc tctttttctg
     7381 taagagtttc tgtatctttt tcatcaatgt atacttttcc ttctgtgggt ttatcaataa
     7441 gtccaagcag atttaataaa gtagttttac ctgaacctga tggtccaatt atgacaacaa
     7501 tttcaccctt ttttatttct atatccacat ccttaattgc gataactcca ttataatttt
     7561 tggtcagttt ttctcccttt attttattca tttcttattg cctctgctgg attaagtttg
     7621 gatgctctct gtgccggata aatacttgct atacaactaa ctattataac aagaaagaaa
     7681 atccagattg tatcaaaaag tgaaatttca attggcagtt tagaaatatc ataaacaaat
     7741 tcaggcagat ttataaaatt atatttttta agaatccaag aaagcaaaaa tccaaaaatc
     7801 ataccgaaaa tcaacccttt aatcccgagg taaattccct gatatagaaa aatctttttt
     7861 atttctccag atgttaaacc cattgccttt aatataccta tttctttaac ctttctgtaa
     7921 acagatatca ttaaagtgga aagaatatta aaggaagcaa ctataactat caaacttaaa
     7981 attattgcca ttgccttttt ttcaagtgct attgctgaaa aaagtatttt gtttttttct
     8041 atccatgttg aaatactata attttcatca attaattttc ttatcttatc cgaaataaca
     8101 ggtgttttat aaatatcatc aactttaact ccaattccat gaactccact catattaaag
     8161 atttccataa ggttttttaa agaagtaaaa cccatactca catcaaaact gtaaactcca
     8221 tattccacaa ttccaacaac tttaaaaatt ctttctttag gaataagtcc tgctattatg
     8281 aatatataat caccaacttc aacaccgagt tcacctgcaa gttctttccc aagtataatt
     8341 ccattttcaa tttcttcaat agaacccttt tttatgaaat ttttaatatt tgtaacatct
     8401 gtttctcttt ttaaatcaat acctttaata atccctccaa gaatatacct gtttgactta
     8461 taaattacct gagacattat gtatggagaa acacccttta tttcaggaag atttttaact
     8521 ttttcaatta tttcttcata attgtagatg taaggttttc cctcaattgt tattaaagga
     8581 ttggctccaa ttattttcct tttcaggtca tttgaaaaac cattcataac tcctataaca
     8641 agaagtaaag taacaactcc tattgaaata ccaagaacag taaaaaaact tataaaccac
     8701 agaaatctat cctctctttt atttccgata aatttttttg ccagaaaaaa attataagaa
     8761 atcatttatt taatatttta atttcgcttt ttttctcaac aattttcaga atttctggta
     8821 tatctgtaat ctttaaaata ccttttggga aattagaaga cgggagttta gtatagggtt
     8881 tttttataag agaataaaaa gaatcaggaa gtttatttaa attaattctt tcaatcaaat
     8941 cagaaaataa acatacaaac aaagatatga ttgctccctg tctatttcca tatagattta
     9001 ttcttttaca tccaagataa ttgagcaaat ttataacaga aagtacatca tatattctct
     9061 taccgagata actttctcca aataaaagat aaaaaccatc aaacatataa tcaaatctat
     9121 aaggatggaa aaaatatttt ttaatgtcag gcattgattc accaattcct ctcacatcta
     9181 caaaataatc agaaggttta ccatcaggaa atctttttat ctcatcctca gaagaaatat
     9241 caggaacata caggtttatt tcttcctcaa cttcaagata atatggtttt ttcacatttc
     9301 ttctttttaa aattacccat atattttctt ctgtctcaac tgcatatctt ccaatagatt
     9361 cattctttat agaaactggt cttaaaactc tgtaatgcgg aatacttata ctctccggaa
     9421 tagaaagaat cttttttaat tcatttttta aattgctttc atcaaaattt tttctattct
     9481 taattataat ttctgttttc tcccttataa tttcaaaatg tggctttgaa ccctctaaaa
     9541 gaacatttcc cttttttgtt gcaaaaagtg ttttttcttt ttcaataatt atatcaggtt
     9601 ctacatttaa aacgtttttc ccaacaattt tacagaaaaa agaaaccatt gcattctgag
     9661 aatctgaatg ataaccatgg gtattatccc cgataaaata agtaaagttt tcttttttat
     9721 caaaaatatt ataaaaatat tcaagttctt cacatatttc tttaaaaccc cttatatcaa
     9781 agtaatcata tttctggcca agcagtatta aaggtgatgg tacacgagag ataaagaagt
     9841 cagaaatttc aaccccttct ccaagaattc caggtggata ctgctcacta tcctgtggta
     9901 attcattttc aagattatat aaaagaggtg ttacaaaaca gccaggagca gcagttttca
     9961 atctttcatc aattgcccat aaccatgttg ttaatgtacc accaccagaa tttcctgtta
    10021 ttccaagaaa atttttatcc cattcatctc ttgaagttat ataatcaatt gctcttattc
    10081 catcccatac cctgtatgaa ccaaaaaatt cacctataag attcatctgt ttacctatca
    10141 tattatgtgc aagacagttt ccttttttca caggtaattt ttcaggcaaa aaataatact
    10201 ggtttctttc cccctggtca aacggatcat atatcaaaac taaaaaacca ttatgaacaa
    10261 gtcgctgaca aaattcctga tatttaggtt ctgctttccc ttctattgaa tgtccacatg
    10321 gtgcaacaac agcaggaaaa ggtggattaa atttctcagg tatatataaa tttgcagtaa
    10381 caaaacatga aggcatactt tcaaaaatta ttttttcaat cctatatcca ttcctttcta
    10441 ttataccagt tatttttgga tttaatggtg tcttttcgga taaagggaaa ggagaaaatg
    10501 cttttttaat tttttctcta acatattcct gatatttttc tgcatctttt ctatttttta
    10561 tatcctttaa tctttttttt ctttcataat aaaccttcct tgttctttta ataaaataat
    10621 caaacatcat atgtctgtat tcattaagca tttttatccc ctgtaatcaa taatccttaa
    10681 aaatgattct actttaagac atgctcctcc aacaagaact ccatctatat ttttttcttt
    10741 cattagttgg tcaatatttt ctggctttac acttcctcca taaagaataa ttaagttttc
    10801 ggctatatct ttgttataaa tttttgctat taattccctt ataaaaatgt gaacttcttc
    10861 tgcctgagat ggtaaagcag tttttccagt tccaatagcc catacaggtt cataagcaat
    10921 aacaagattt tcaaatttat caaaaccttc cagacatctt ttaacctgat tttcaataac
    10981 attaaatgtc tctccttttt ccctttcttc aagtttctct cctacacata aaattgcatt
    11041 cattcctgtt tctacaacct tttttacctt tttgtttata atttcatctg actcaccaaa
    11101 aatatttctt ctttcagaat gcccgcatat aacatattca acacctgtat ccttaagcat
    11161 taaaggtgaa atttctccag tataagcacc ttcattttca aaatatacat tttgagcacc
    11221 aagtttaaca gcactgtttt ttattatttt agatacagga taaagagatg taaaaggagg
    11281 acatattaaa acatccctat cctcataact tttaatgttt ttaacaagtt cactcacaaa
    11341 atttattgat tctgctgggg ttttatacat cttccagttt cctgctataa gagatttttt
    11401 cactttcttc tcctttttta tagtagtaaa aagtgtggtt ttataatttt tactcttcct
    11461 ttaaaacttc aattccgggc agggtccctt tttcaagaaa ttccagagat gcacctcctc
    11521 ctgtacatat atgggatatt ttatctgcaa ctcctgcttc atcaactgct gcagcagaat
    11581 ctcctccacc aataactgaa aatgcttttg aatttgcaat tgcctttgct aattcaatag
    11641 tcccttttgc aaatttctta atttcataaa ttcccatcgg tccattccag aaaattgttt
    11701 ttgctctgtt tatacaatca gtgtattctt caattgcctg aggacctata tcaactccaa
    11761 tccagccatg tggaactgtt cccctttctg ttattattgg aactgcatct tctgaaattt
    11821 tctgaactat aacatggtca agaggcgtat gaaattcaag atgtctttta tttatttctt
    11881 taattatctc ttttgcaaca ggaaccgctt cttcttcaac ttttgaatca cccacttccc
    11941 agttttttgc ttttataaat gtgtatgcca taactccacc tgtaatgatt gtatcaactt
    12001 tatcaagaag atttttaacc atatttattt tatctattac cttcgcacca ccaagaatta
    12061 aaagaaatgg tttttcagga tttatcagta attttgaaag tgattcaact tccttttcaa
    12121 taagaaatcc tgctgctaca tcctttgcaa attttgttat ttgataaact gatgcatgtt
    12181 ccctgtgaca tgttccaaaa gcatcattaa cataagcatc tgcaagagaa gcaagttctt
    12241 ttgcaaaatt ttcatctcca ttttcttctt ctgaatgaaa tcttaaattt tcaagaagta
    12301 aaatttctcc ctctttcaat tcatttacca ttttttttac ttcctctcca atacaatcag
    12361 gagcaaattt tacctcttta tttaaaattt ttgacaatct ctcagcaaca ggttttaaac
    12421 tgaattctgg agatggtttt cctttcggtc ttccaagatg tgaggcaagt attaaagaag
    12481 caccttgaga aagtaaataa tttatagttg gaattgttgc tgtgattctt gtgtcatctg
    12541 tgataactcc tttttcaatt ggaacattat aatcaactct cagaaaaact tttttccctt
    12601 tataattcct gtttttaatt gattttattt tcataattac ctcctttttt tctttatttt
    12661 acaatttttt atatcaatct gtccactaaa aaactaaatt tcatattcat caagatatga
    12721 aggtataaaa atctttgaat ttttaaatct atcataaata acttttgaaa attcatcaat
    12781 tacttcttct tctccatgaa ctaaaaatat actttctggg aattttttaa atcctgaaag
    12841 aaaacttaaa agttcttctc tatcagcatg ggcagaaaat ccatctattt tctcaatttt
    12901 tgcctttact ttataaaaat tacccaaaat cctaacttct tttttctcta gtatttccct
    12961 tccaagagtt ccttttgctt gataaccaac aaaggcaata gatgattctt ccctttcaat
    13021 attattaaca agatggtgct taattcttcc gcctgtacac ataccactgc ctgccataat
    13081 aattactgtg ccctttatat gattaatcat ttttgactct tcaactgact ttgtaaaatg
    13141 taaaagtggg aaatcaaaag gagaaatttt atttttaagt agattttctg cttctccatc
    13201 aagatatgtt agattttttt tgaatacttc tgttacttca attgccatgg gactatcaag
    13261 aaatgtaaat aggtgtggta ttctatcttc aagtaagagt tttttcaaat agtataaaat
    13321 ctcctgtgct ctttcaatag caaacgtagg aataacgata ttgccttctt ttctttcagt
    13381 ttcatttatt attctttcaa gtttttcttc agcaatttct cttggctcat gaattttatc
    13441 tccataagtc gtttccataa tcagaatatc agtactctct aaaatttcag ggtctgatag
    13501 aattggattt tctttccttc caatatctcc tgaaaaaact atctcttttc catctgctat
    13561 aacttttatg aaggaggaac caagaatatg acctgaatta taaaaaacaa cctttatatt
    13621 gtccgaaatt ttgacttcct cattatatga aaccgtctca aaaaaatcaa aaacactcat
    13681 tgcatcatta acatcataca gaggtatttc aggataggga ccttttcttc cttccttttg
    13741 atgtcttttt ctcttttttt cagcatcttc aacctgtaac tttgctgaat caagcagtgc
    13801 tatttttgtt atttctgctg ttggaacagt acataaaatt tttcccttaa atccatcttt
    13861 tacaattttt ggtaaatatc ctgaatgatc aagatgtgca tgtgttaaaa gaatataatc
    13921 aatttctaaa ggtggaactg gaaatttctc ccagtttcta ttttttaaat ccctttcctg
    13981 aaaaagaccg caatcaatta ataaattttt cccaccaatt tgtaagagaa attttgaacc
    14041 tgttacattt tttgtcgcac caagaaattt aattctcata aactttctcc tttttctttt
    14101 ttaataagag aaataattga aatcgctaca attgcatgaa aaatagaagt tatcaaaaat
    14161 ggagttctta tatttataat ctttcctgta tatccaccta taagaggacc aaaagcaagc
    14221 ccgagcatac ttattgaagt aaaaagacca tatgctttac ctattgagtt ttcatttata
    14281 agagttttta tcattgaatt gacagaagga attacaccag caacactcgc aacaaataaa
    14341 accctccaga aaaaaagaga aaaaactgaa tgaacaaaaa aatgtcctaa agatgcaatt
    14401 gaagcaaaga taaaacaatt tcctatcatt tttatatatc caattctatc gctcaaattt
    14461 ccaagtccag cagcacttaa accctcaata atagcagcaa tccctataat tgtccctgta
    14521 attgtattca atttttcggg attacttatt atttccttaa ttacaagagg gaaagaagga
    14581 tttaaaattg tattactcaa tctgacacca aacataatca ttgttgctat ccagaatcct
    14641 tttaacttta atatttccct gtatgaaaat tcttctttct tcttataatt tattctgttt
    14701 tcccttgcaa ataaaagaat taaaagacct ccaagaaaaa ttataagacc tccaattcta
    14761 aaagtatttc tatagccaaa atggtctgaa ataattccac ctataagagg acctactgaa
    14821 tttcctattg aaacagcagt ttgcatcatt ccaagagtaa aaccactttt ttccacaggt
    14881 acaacacttg ccacaagagt aacagaagca acaattgttc ctgtcaaagc accttgaagt
    14941 aacctgcata tcaataattg tttcactgtc ctaacaatag acattaagaa taaaataaaa
    15001 gtccctgcaa acatacttct aacaaccatt aattttcttc catacttatc agcaaaatat
    15061 ccccagatag gtgaaaaaat tgtaaatgta aaaccagaag cagatagaat tattccagcc
    15121 cagtaagttt gagaaggtat atcctttatg ccaagttcag aaatgaaaaa tggtaaaaat
    15181 ggcatagcaa aggaaaatcc aattattgaa aaaatctgcg caataaaagt tgaaataaaa
    15241 gtttttttcc acatttttga tttttaaaat tttttacaat tttacaattt ttaatttttt
    15301 taagcaaaaa acact
//
LOCUS       DAOSAT010000040        15205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2675_length_15205_cov_5.494719, whole genome shotgun sequence.
ACCESSION   DAOSAT010000040 DAOSAT010000000
VERSION     DAOSAT010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15205
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2675_length_15205_cov_5.494719"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PLF90_04670"
     CDS             <1..695
                     /locus_tag="PLF90_04670"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK56607.1"
                     /translation="AGACQTMININRVLPGKKVLMVGSGNVGLIISYQLLQAGADVCG
                     VVEIMPDIGGYLVHAAKIKRFGVPIYLSTTVLEAVGKKEVEGAVICPVDNDFKPILRK
                     RKEIDVDLICIAVGLTPLNELLYQAGCLFTYKVYLGGIVALHNENMETSVNGIYIAGD
                     LSGVEEASTAMEEGKLAGLSVAERLNKKSHKDEKEEVLKRLNDFRIGTFGEKRKIAKE
                     EIIKEYYERVEN"
     gene            676..1179
                     /locus_tag="PLF90_04675"
     CDS             676..1179
                     /locus_tag="PLF90_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK56608.1"
                     /translation="MKELKIDEFFYLKKSGYPSESRIKKGPVAIIECVEEIPCNPCET
                     ICPKGAIKVGVPITNLPELNEEKCIGCGRCIPVCPGLAIFVLDYNFTKKEVSLSIPYE
                     FLPLPENGDIVKCLDRNGKYVCDGKIVKVIPPEKNNMTGIITFSFPKKYYNFVRSFKI
                     KKWKRKK"
     gene            1161..1433
                     /locus_tag="PLF90_04680"
     CDS             1161..1433
                     /locus_tag="PLF90_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOK56609.1"
                     /translation="MEKKKIICRCKEITEEEIIEIIEKGIVDVDGIKRALGAGMGLCQ
                     GKTCSILIAKIISKKTGIPLEEIRQQTSRAPVRPISVKTISELEER"
     gene            complement(1616..2782)
                     /locus_tag="PLF90_04685"
     CDS             complement(1616..2782)
                     /locus_tag="PLF90_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK56610.1"
                     /translation="MNSRERVKILLKKEIPDRMGIYEHFWPETFCGWVKEGYPVKRVH
                     KKQEESRWDEETGRYVKVENEGDYIEPVNPGEYFDYDIVNCGGWFDTSLFPGRSEVLE
                     ETDEWQIIKDGRGAILKWWKKKCGTPEHIDFEIKTSEIWKKYREILFETKKERLGDLN
                     AIKENLKKAKEKGKFSVFGNLFVFELLRATIGDQNFLPALLLEPEWIKDFCQLYTDFY
                     IRHYEILFREVGTPDGFFLYEDFGYSKGLFCSPKTLEELIMPYEKKLVSFFKDYGLPV
                     ILHSCGDVRKAISLIIDAGFDCIQPMEAKAGFDVVKLAEEYGKKIAYMGNINVVILGT
                     NELNKVEEEVLRKVKKLREMRIPYFFHSDHSVPPTIKFKTYTHAVKIFRENSYY"
     gene            complement(2791..3762)
                     /locus_tag="PLF90_04690"
     CDS             complement(2791..3762)
                     /locus_tag="PLF90_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK56611.1"
                     /translation="MKIGIIGTGRISNVHINSIKKIEGIKIISVCDIIEEVAKNKAKD
                     IGCSYYTEYKEMIEKERPDCVWICTPADTHTEIVLYCIEKNLPFFLEKPPALKEEECE
                     IVIDKLKTKNIIHSIGFMLRYDPASEKLKEIIKNEKVIFISSEWFWTIPLVDSIKIKE
                     KAGGQIVDQAIHFIDLIRYVFGEIKSVYTKRVRGFFPEEKLYTGDDASCTIFEFENGI
                     YGNLLCTYALFPEVTRYYPPKIRFICKRKLIEYSHRKLIIITSEKYEEFNFNQDLYFV
                     EDRVFIESLRKNDKSIIKSDYFDSIKTLKVALSANKSMEENREIIIR"
     gene            complement(3759..4856)
                     /locus_tag="PLF90_04695"
     CDS             complement(3759..4856)
                     /locus_tag="PLF90_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014935274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOK56612.1"
                     /translation="MERLEKKLEEKLVKLGQIIVNQEKARIIVEPYRNEKGYWFGGGK
                     IRKDIEGRIVLTGRYRNSGDSRYGIKMGERGLELAIFISEDKGKTFKKLKSFSKKELK
                     VGKKEVLSIEGSSIYFGDKVEVYVSTEKKRNYPEGFEIYQKEGTGIWEIDVFEGNTIE
                     DLNPENIRNVLISENPENLHVKDPFVFDLNKKTYMIFCQHPFSWTCSYSGLAVKENDI
                     FKIISKDILPKGYTWDIAVTRITERLPVPKIGIFKDIPDISLYFYDGAECIREHPQSE
                     KGVKRPRGYSCEEIGGLAYGFDYEFPKIYRLSLNFPLFISPFGTGCNRYVSAFFDGEK
                     IYAIWQRSMPDFSQPLVMNTVDIEEIERILK"
     gene            4942..6309
                     /locus_tag="PLF90_04700"
     CDS             4942..6309
                     /locus_tag="PLF90_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOK56613.1"
                     /translation="MNLIFIMIDSLRQDHVSVYNKGERVFENIESCKTPNIDKFAEKS
                     LVFENVYPCGLPTIPVRYELMTGFYSLPYRPWCPLTDYDLTIAQILNKEGYLSGFITD
                     TYHYRAPNMNYHRNFNVYEWIRGQEYDPYEIPVSKRNINDYVNENYTDEWKKRILQFL
                     ANTDNMNEKNWFTMQVVEKAIEFLKKARKTNQKIFLWIDSFEPHEPWDPPEKFDKYTS
                     KKYKGKRLIMPMGGEAKNWATEEEIDFIRGLYAGEVSFVDYCFGYLFKELERLNFYED
                     SLIVLTADHGHPLADHGKFLKGSDRMYSELLKVPFIMKFPEELYKGKIDALIQFPDVL
                     PTILEILGFEGNNVCLSGKSFFSVIKGDIDKHRDAVICGYKRGIYRCIRNKEWSFVYT
                     PDEEIDMLFNLKDDPKERVNLIDKHPDIAKELLNKFGSVFFEKKSKVIKGIQGEYELA
                     SSSIE"
     gene            6306..7664
                     /locus_tag="PLF90_04705"
     CDS             6306..7664
                     /locus_tag="PLF90_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOK56614.1"
                     /translation="MKKLNILFILIDDLGWKDLSIYGSEFYETPNIDKIAEEGIIFTN
                     AYSASPVCSPTRASIQTGKYPARLGITNYIGGRDRGKLLPPENLEYLPFEEETIAKYL
                     KKFGYKTYYIGKWHLGDRPYFPENHGYDKNIGGCSWGMPHYGYFSPYKIPTLEDGPEG
                     EYLTDRLTDEAVKIIKETEEPFFMFLSHYAVHVPIQVPEKYIQKYREKAKKLKLDKIN
                     PFIEGENFPCEHKKHLKIIRRIIQSDPFYAGMIENLDENIGRVIETLKETGKYENTVI
                     IFTSDNGGLSTAEGSPTCNLPLREGKGWIEEGGIRVSLIMRIPGIKKQKCDFPVVSTD
                     FYPTILDITGLPLSPHQHKDGISILPVLRGEDLKRNGLFFHWPHYGNQGGTPSSSVIY
                     DNWKLIYSYEYEKYLLFNLNEDREEKNDLTDKKTKEFKLLKELLDHYLKDTKAKLPVK
                     NG"
     gene            7657..8754
                     /locus_tag="PLF90_04710"
     CDS             7657..8754
                     /locus_tag="PLF90_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOK56615.1"
                     /translation="MDKIELLVKPTSYLCNLDCSYCFYKKTKQIYPEAKIMDLKTLEF
                     FIKKFMDYSEGKNIFFCWQGGEPLIAGIEFYYNVVEFQKKYGKTGQIVGNTIQTNGIL
                     IDDKWIEFFKRYNVFVGISLDGPEEIHNFYRGYKDGKKTFKKVMENINKLERNGVQFN
                     ILSTIGEETGKYPEKIFEFFVKKGFKYLQFIPATDRKKDKISKFSITPSTYSNFLCRI
                     FDLWWNNGFPYVSIRFFDNIIEIIKGIEPSACTLKKRCGEYLVVEHNGDVYPCDFFVN
                     TDFKLGNIFFDELDIIYEKLKKFGAMKEIQIEKCKKCEWNFICNNGCLWHRYVKNGKL
                     DGTDYFCFAYRKFFTYSFEKFKIISERVKLF"
     gene            8767..9897
                     /locus_tag="PLF90_04715"
     CDS             8767..9897
                     /locus_tag="PLF90_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56616.1"
                     /translation="MKREIKIIKDFPMANVCDIEIEEKEIPIISFSPSPKGGPECLWF
                     YFKVKSKGIKKLKLILKNTGNMLGGDRPENLRPVIKFEKKDWERLSKPELIEFEDGRK
                     HVSWNVNIMSEKFEIAFCYPYGKKEIDEIIKESKGYWKKDVIGISQEEREMIRLSNSY
                     GDIKKKGVYLMARQHSGETPGSWVLDGFLRYLSKINYKNLIVWAVPLSNIDGIENGYY
                     GKDNFPYDLNRAWGFPPMRHETLVIQRDIMRWKERCIPFLAIDFHAPGACEIDGAYFF
                     ITPFEKDKKMYKIEKEMTEKIAKKVGKYMSENYIRSINYKSRWETPHFSDFIRNNIKI
                     PSLSMETPYCICRNCLMKKEDYKKIGKKIALGIIEEIEKYGT"
     gene            9887..11041
                     /locus_tag="PLF90_04720"
     CDS             9887..11041
                     /locus_tag="PLF90_04720"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOK56617.1"
                     /translation="MGLKIGTFKEKLKIEEGSKLAGYPNTETRFCKGSKGDIEANGII
                     LKNDKNFGCLVSLDILGIELPFTEKIREKVEKEFKIPFDNIMICCTHTHSSVITVGRK
                     FGADKEFLNEIERKILKGIEDGFKNIEEVNLKWTKTEVDISHNRRVVIDGKAKNEWQD
                     IEKKHTGIVDEEVICIGFFKKNGDLKSLIVNYACHPVVLGPSNYYASADFVGYLRSFL
                     ENNLNLENLVYITGASGNINPRICITDNFEVAKKTGETIGEQILKCKENFHNLEVNDF
                     HFDRKKVVFIKKDGTGILETEMQILKIGEKIIFITLPGEPVVEIGLDIKKKSKYPVTI
                     VAGYTNDYIGYISTDKILSEGGHEANSCPVKEVEKTIKEIVKNYMKNGRT"
     gene            11028..11789
                     /locus_tag="PLF90_04725"
     CDS             11028..11789
                     /locus_tag="PLF90_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK56618.1"
                     /translation="MVELNTAIEIKFIHLSFETGIEAPHFHEENQLLFFISGKGIETV
                     ENKKYKILPGTFILIPEKRVHFFKSTLNKPAEILSLRFKLGQKIADETFTDFFKKTYI
                     YRGGKENIKTIKKMIPSIINGKPNFSFLLKILNMIFETSTKTPEKTTEYMEKLEKYIE
                     ENIGSEIKIKKTAEYLGISEVYVRKLVKKIYDISFTKFVNIKKIEYASKLLVETKLPV
                     SKIAGMCGFYDFNYFSRVFKKIKRVSPVKFRKSYL"
     gene            11804..13819
                     /locus_tag="PLF90_04730"
     CDS             11804..13819
                     /locus_tag="PLF90_04730"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOK56619.1"
                     /translation="MVKKIFLILFMFIFCLEAETIWVDCSSKKDKETGTKEFPFKKIS
                     DAIKISKEGDEIIIKQGIYRETVQPKSGIKIKGEGRVIISGYEKIKGWEKFEKNIWKT
                     YLNREIDELFIENKRIEIARYPEKNWFKVEKFEEKGDIYRIYNSEIKNLKDVKNFEVR
                     ILNSTVNGFFTCPVINFNKEEGFFEFAKQPYLKLTEKDLFYLQHSPNFITSPGQWAIE
                     KENDRYVVYIWPQNEKELNFIETTGKEDRILILSRVKNVEIDGIEICGSNTNGLWIDN
                     SENITIKNCIIYNNKNVGLYVNRSKNTKIMKNIVLFNGNGISVGNSSDIEILNNEIAF
                     NNIDGLIVSWNSENVLVKGNYIHHHILYGHPDNMQTYRNVKNLKIEDNLLICGGQSIM
                     MEETYDVELKNNIIMGAGAYSLIFGHQNTDNVKMYFNTVVLSQYGSISFTGKKYDVKG
                     NIFITGHPGAIYGINVNEYEGDYNIFWNCEIQNPTVIAASGKWYKLSQFEVYKKDTQK
                     DLNSLYSNPMFKNFPKSFHIVEPTLSDEEKICIVSQTDLINEGDFVEINFDGIPRKVI
                     AKEGKYIKISPKLEKVPFRIVMVCNWGRNDNFEYNFEFKDESPVKKVLYENQTPGSKI
                     NFKNIKNYDFDGDGKRDLPLLSEDLDKVYNEKMKKTYLGGEIEKKWK"
     gene            13823..15145
                     /locus_tag="PLF90_04735"
     CDS             13823..15145
                     /locus_tag="PLF90_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOK56620.1"
                     /translation="MKKIYLFLILLLKISFSEVYYVSINGSDENPGTKEKPFRTIQKA
                     ANVVNPGDTVLVLPGEYNEKVNILKSGQPEKYIKFKGEGKVEEIKTISFQIGGWAPRK
                     ANYIIIENFYLSGGVSISGDYNIVQNCILEGYGVSISFHPTSHPDSTGCIVRNNIMRN
                     FGKVVLNCTGTKSSNCIFENNVIYNGEGDVWRIFGKGHIIRNNEVYDLRETGWHADFF
                     QIYDNNREISYDILVENNYFHDSTGSIAMLLSYGYSDLRDWTFRNNIFYNIGGVAQIG
                     IPGVKFYNNTFVNCGRNTAGPLLFRYYEKKGEFEVGGHRARIFNNIFIGGGSYPDSDE
                     IGWYHFADKPKEEIKDFQADFNYVTKTKDANYAPKNEFSKVEKNGINGGDPKFVNFEK
                     KDFHLLPDSPAIDRGTEIKDWIDPKDKDGIKRPSGSKWDIGAYEFEKN"
BASE COUNT         5716 a         1874 c         2547 g         5068 t
ORIGIN      
        1 gagcaggtgc atgccagaca atgataaata ttaacagggt gctaccagga aaaaaagttt
       61 taatggttgg ttctggaaat gttggtttaa tcatttctta tcagttatta caggcaggtg
      121 cagatgtttg tggtgttgtt gaaattatgc cagatatcgg aggttattta gtccatgcag
      181 caaagattaa aaggtttggt gttcctattt atctttcaac aacagttctt gaggcagtgg
      241 ggaaaaaaga ggtagaaggt gctgttatat gtcctgtaga taatgacttt aagccaattt
      301 tgagaaaaag aaaagaaatt gatgttgatt tgatatgtat tgctgttggt cttactccat
      361 tgaatgaact tttatatcag gcaggatgtt tattcacata caaggtgtat cttggaggaa
      421 tagtggcttt acataatgaa aatatggaaa caagtgtaaa tggtatttat attgccggag
      481 acctttcagg tgttgaagaa gcatcaacag caatggaaga aggaaaactt gctggtttat
      541 ctgttgcaga aagattgaat aagaaaagtc acaaagatga aaaggaagaa gttttaaaaa
      601 gattaaatga ttttagaata ggtacatttg gggaaaagag aaaaattgct aaagaagaaa
      661 taataaagga atattatgaa agagttgaaa attgatgaat ttttttattt gaagaaaagt
      721 ggttatccat ctgaaagtag aattaaaaaa ggacctgttg caattattga atgcgttgag
      781 gagataccat gcaatccatg tgaaactata tgtcctaaag gagcaattaa agtgggagtt
      841 ccaataacaa atttaccgga attgaatgaa gaaaaatgta ttggttgtgg aagatgtatt
      901 cctgtctgtc cagggcttgc aatattcgtt ttggattata atttcactaa aaaagaagta
      961 tctttatcaa taccttatga atttttacca cttcctgaaa acggagatat tgttaaatgt
     1021 cttgatagaa atgggaaata tgtatgtgat ggaaaaattg ttaaggttat tccgcctgaa
     1081 aaaaataata tgacaggaat tataacattt tcatttccca aaaaatatta caattttgtg
     1141 aggagtttta aaataaaaaa atggaaaaga aaaaaataat atgtagatgt aaggaaataa
     1201 cagaggaaga aataattgaa attatagaaa aaggcattgt ggatgttgat ggaataaaaa
     1261 gggctcttgg agcaggtatg ggtttatgtc agggaaagac atgttcaatt ttgatagcaa
     1321 aaataatttc aaaaaaaaca ggaataccat tggaggaaat aagacagcaa acaagtagag
     1381 cacctgtaag acctatttct gtaaaaacaa tttcagaatt agaagaacga tagatttaat
     1441 tattttgatt ttgtacattg gagaagtgat tatttatgta tgattcataa agacagaaga
     1501 agttaaaacc ttttttctac cttttacctt tctcatgttt tttatcccaa ttgaataagt
     1561 ttttaaaagt tattttttaa tatttttaat tttttctacg tctcattttt tgttcttaat
     1621 aatatgaatt ttctctgaat atttttactg catgggtata tgttttgaat tttattgttg
     1681 gtggaactga atggtcagaa tggaaaaaat aaggtattct catctctctc aatttcttca
     1741 cctttctcaa aacttcttct tcaactttat tcagttcatt tgtaccaaga attactacat
     1801 ttatattccc catataggca atctttttcc catattcttc tgcaagtttt actacatcaa
     1861 atcctgcttt tgcttccatc ggttgaatac agtcaaaacc agcatcaata attaaagaaa
     1921 ttgcttttct tacatctcca caactatgaa gaattacagg caacccataa tctttaaaga
     1981 aacttacaag ttttttctca taaggcatta taagttcttc aagtgtttta ggtgagcaga
     2041 aaagtccttt tgaataacca aaatcttcat aaagaaaaaa tccatctggt gtaccaactt
     2101 ccctgaataa aatctcataa tgccttatat aaaaatctgt ataaagttgg cagaaatctt
     2161 ttatccattc aggttcaagt aataaagcgg gaaggaaatt ctggtctcct attgttgctc
     2221 ttaaaagttc aaatacaaaa agatttccaa atacagaaaa tttccctttt tcctttgcct
     2281 tttttagatt ttcttttatt gcatttaaat ctcccaatct ttccttcttt gtctcaaaaa
     2341 gaatttctct gtattttttc catatctctg atgtcttaat ttcaaaatca atatgttcag
     2401 gtgttccaca ttttttcttc caccacttta aaattgctcc tcttccatct tttataatct
     2461 gccattcatc tgtttcttca agaacttcac ttcttccagg gaaaagagat gtatcaaacc
     2521 agccaccaca attaacaata tcataatcaa aatactcacc gggattaact ggttcaatat
     2581 aatcaccttc attttcaact ttcacatatc tacctgtttc ttcatcccat cttgattctt
     2641 cctgtttttt atgcactctt tttacaggat aaccttcttt tacccatcca cagaatgttt
     2701 caggccagaa atgctcatat atacccattc tgtcaggaat ttcttttttt aataatattt
     2761 ttactctttc tcttgagttc atttttcccc ctatcttatt attatttccc tgttttcttc
     2821 cattgattta tttgctgaaa gtgctacttt taatgtttta attgaatcaa aataatctga
     2881 ttttattatt gatttatcat tttttcttaa actttcaata aatactctat cttcaacaaa
     2941 ataaaggtct tggttaaaat taaattcctc atatttttct gaagtaatta taattaattt
     3001 cctgtgtgaa tattcaataa gtttcctttt acatataaat ctaatttttg gtggatagta
     3061 tcttgtcact tcaggaaata aagcatatgt gcataataag tttccataaa ttccattttc
     3121 aaattcaaat attgtacaac ttgcatcatc tcctgtatat aatttttctt ctgggaaaaa
     3181 tcccctaact ctttttgtat atacggattt tatctctcca aaaacatatc ttataaggtc
     3241 aataaaatgt attgcctggt caacaatctg tcctcctgcc ttttccttaa tttttattga
     3301 atcaacaaga gggattgtcc agaaccactc agatgaaata aatataactt tttcattttt
     3361 aataatttct ttgagttttt cagaagcagg gtcatatcgt aacataaatc caatactgtg
     3421 aataatattt tttgttttaa gtttatcaat aacaatttca cattcttctt cttttaaagc
     3481 aggtggtttt tcaagaaaaa atggtaaatt tttttcaata cagtataaaa cgatttctgt
     3541 gtgagtatca gcaggtgtac atatccagac acaatcaggc ctttcctttt caatcatttc
     3601 tttatattca gtataataac tacatccaat atctttcgcc ttattctttg cgacctcttc
     3661 aattatatca caaacagata taatttttat tccttctatt tttttaatag aatttatatg
     3721 tacatttgag attcttccag ttcctattat tcctattttc atttcagtat cctttctatt
     3781 tcttcaatat caactgtatt cataacaagg ggttgtgaaa aatcaggcat tgacctctgc
     3841 catatagcat aaattttttc tccatcaaaa aaggcagaaa catatctatt gcatcctgtc
     3901 ccaaaaggag aaataaataa agggaaattt aaacttaatc tataaatttt aggaaattca
     3961 taatcaaaac cataggcaag tccaccaatt tcttcacatg aataacccct tggtctttta
     4021 actccttttt ctgactgtgg atgttctctt atacactctg ctccatcata aaaataaagt
     4081 gaaatatctg gaatatcttt gaaaattccg attttaggaa caggcaatct ttctgttatt
     4141 cttgtaactg cgatatccca tgtataacct tttggtaaaa tatcttttga aattattttg
     4201 aaaatatcat tttctttaac agcaagacct gaatatgaac aggtccagga aaatggatgc
     4261 tgacagaaaa tcatatatgt ctttttattc aggtcaaaaa caaaagggtc tttaacatgt
     4321 aaattttctg gattttctga tattagaaca tttcttatat tttcaggatt taaatcttca
     4381 attgtatttc cttcaaaaac atctatttcc cagatacctg taccttcttt ctgatatatt
     4441 tcaaaaccct ctggataatt tctctttttt tctgttgata cataaacttc aactttatct
     4501 ccaaaataaa ttgatgaacc ctctattgat aagacctcct tttttccaac tttaagttct
     4561 ttttttgaaa atgattttaa ttttttaaat gttttacctt tatcctcaga aataaaaatt
     4621 gcaagttcaa gccccctttc tcccattttt attccatatc tactatcccc tgaatttctg
     4681 tatcttcctg ttaaaacaat ccttccttct atatcctttc ttatctttcc acctccaaac
     4741 caatatccct tttcatttct atatggttca actattattc ttgctttctc ctgatttact
     4801 attatctggc caagttttac aagtttctct tctaattttt tttctaatct ttccatttta
     4861 atctcaaaag gtttaatttt ttattttaac atatttgaaa ttttttttgg aaagattata
     4921 attttgaaaa aaggaggaga gatgaattta atttttatta tgattgacag tttaaggcag
     4981 gaccatgtga gtgtttacaa taaaggggaa agagtttttg agaatattga aagttgtaag
     5041 acgccaaata ttgataaatt tgctgaaaaa agtttagttt ttgaaaatgt ttatccatgt
     5101 ggtctcccta caatccctgt tagatatgaa cttatgactg gtttttattc attaccttac
     5161 agaccttggt gtcctttaac cgattatgac ttaacaattg ctcagatttt aaataaagaa
     5221 gggtatctct caggatttat aacagataca tatcattaca gggctccaaa tatgaattat
     5281 cacaggaatt ttaatgttta tgaatggata agaggccagg aatatgaccc ttatgaaatc
     5341 cctgtttcaa aaagaaatat aaatgattat gtgaatgaaa attatacaga tgaatggaaa
     5401 aaaaggatac tccaatttct tgcaaataca gacaatatga atgaaaaaaa ctggtttaca
     5461 atgcaggttg ttgaaaaagc aattgagttt ttaaagaaag caagaaaaac aaaccaaaaa
     5521 atatttttat ggattgattc ttttgaacca catgagccat gggacccacc agaaaaattt
     5581 gataaatata cttctaaaaa atataaaggt aaaagattga ttatgcctat gggtggagaa
     5641 gcaaaaaact gggctacgga agaagaaatt gattttataa gaggtcttta tgcaggtgaa
     5701 gtaagttttg ttgattactg ttttggatat ttatttaaag aacttgaaag attgaatttt
     5761 tacgaggact ctttaattgt tcttactgct gaccatggac atccacttgc tgaccacgga
     5821 aaatttttaa agggttctga caggatgtac agtgaattat taaaggtccc atttataatg
     5881 aaatttccag aagagttata taaaggaaaa attgatgcat taattcaatt ccctgatgtt
     5941 cttcctacaa ttcttgaaat tcttggtttt gaaggaaata atgtatgtct ttctggtaaa
     6001 agtttctttt cagtaattaa aggtgatata gataaacata gagatgcagt aatatgtgga
     6061 tataagagag gaatttacag atgtatcaga aataaagaat ggagttttgt ttatacacct
     6121 gatgaagaaa ttgatatgct ttttaatttg aaagatgacc cgaaagaaag agtaaattta
     6181 atagataaac atcctgatat agcaaaagaa cttttgaata aatttggttc tgtattcttt
     6241 gaaaaaaaat caaaggtaat aaaaggtata cagggtgaat atgaacttgc atcctcttca
     6301 atagaatgaa aaaattaaat attcttttta ttttaattga tgaccttgga tggaaggatt
     6361 taagtattta tggcagtgaa ttttatgaaa caccaaatat tgataaaatt gcggaagaag
     6421 gaattatatt tacaaatgct tattcagcaa gtcctgtatg ttctcctaca agagcaagta
     6481 tccagacagg taaatatcct gcaagattag gtataacaaa ctatatcgga ggaagggata
     6541 gaggaaaact tttaccacct gaaaatcttg agtatttacc atttgaagaa gagacaattg
     6601 caaaatattt aaaaaaattt ggatataaaa cttattatat aggaaaatgg caccttggag
     6661 atagaccata ttttcctgaa aatcatggat atgataaaaa tataggtggt tgttcctggg
     6721 gaatgcctca ctatggatat ttctcacctt ataaaattcc gacacttgaa gatggacctg
     6781 aaggagaata tttaacagac cgacttacag atgaagcagt aaaaataatt aaagaaacag
     6841 aagaaccatt ttttatgttt ttatcccatt atgctgttca tgttccaatt caggttcctg
     6901 aaaaatatat ccagaaatac agagaaaagg caaagaaatt gaaacttgat aaaataaatc
     6961 cttttattga aggagaaaat ttcccatgcg agcataagaa acatttaaag ataataagaa
     7021 ggataattca atcagaccct ttttatgctg ggatgataga aaatcttgat gaaaatatag
     7081 gaagagtaat agaaacattg aaagaaacag gaaaatatga aaatacagta ataattttta
     7141 catcagataa tggaggactt tcaactgctg aaggttcacc tacatgtaat ttacctttaa
     7201 gagaaggaaa aggatggatt gaggaaggag gaataagggt ttctttaata atgaggatac
     7261 ccggaataaa aaaacaaaaa tgtgattttc ctgtagtttc aactgatttt tatccgacaa
     7321 tacttgatat aactggattg cctctgtctc cgcatcagca taaagatggg ataagtattt
     7381 tacctgtttt aagaggagaa gatttaaaaa gaaatggtct tttttttcac tggccacatt
     7441 atggaaatca gggtggtact ccttcctctt ctgtgattta cgataactgg aaactcatat
     7501 attcttatga atatgagaag tatcttcttt ttaatctaaa tgaagataga gaagaaaaga
     7561 atgaccttac ggataaaaaa acaaaagaat ttaaactctt aaaagaactc cttgaccatt
     7621 atctgaaaga tacaaaagca aaattacctg tgaaaaatgg ataaaattga acttcttgtt
     7681 aaaccaactt cttatctatg taatcttgac tgttcttact gcttttacaa aaaaacaaaa
     7741 cagatatatc cagaagcaaa gataatggat ttaaaaacac ttgaattttt tataaaaaaa
     7801 tttatggatt attctgaagg aaaaaatata tttttctgct ggcagggtgg agaaccttta
     7861 attgcaggaa ttgagtttta ttataatgtt gttgaatttc agaaaaaata tggtaaaaca
     7921 ggacaaatag ttggaaatac aattcaaaca aacgggattt tgatagatga taagtggatt
     7981 gaatttttta aaagatataa tgtttttgtt ggtataagtt tagatggacc tgaagaaata
     8041 cataattttt acagaggata taaagatgga aaaaaaacat ttaaaaaagt gatggaaaat
     8101 ataaataaac tggaaagaaa tggagttcag tttaatatcc tttccacaat tggagaagaa
     8161 acaggtaaat accctgaaaa gatttttgaa ttttttgtta aaaagggttt taaatattta
     8221 cagtttatac ctgcaacaga tagaaaaaaa gataaaataa gtaaattttc aataactcct
     8281 tcaacatatt caaatttttt atgcaggatt tttgatttat ggtggaataa tggttttcct
     8341 tatgtttcaa ttaggttttt tgataatatt attgagatta taaaaggaat tgaaccatct
     8401 gcttgtactt taaagaaaag atgtggagaa tatcttgttg ttgaacataa tggagatgtt
     8461 tatccatgtg atttttttgt aaatactgat tttaaacttg gaaatatttt ttttgatgaa
     8521 cttgatataa tatatgaaaa actcaaaaaa tttggtgcaa tgaaagaaat acagatagaa
     8581 aaatgcaaaa aatgtgaatg gaattttata tgcaataatg gatgtctctg gcataggtat
     8641 gtaaaaaatg ggaaactgga tggaactgat tatttttgtt ttgcttatag gaaatttttt
     8701 acatattctt ttgaaaagtt taaaattata tcagaaaggg ttaaactttt ttgaaaaaga
     8761 tgaaaaatga aaagagaaat aaaaattata aaagattttc caatggctaa tgtctgtgat
     8821 atagaaatag aggaaaaaga aatacccata atcagttttt caccatctcc taagggtggt
     8881 cctgaatgcc tctggttcta ctttaaagta aaatccaaag gaataaaaaa actaaaactt
     8941 attctcaaaa acacaggcaa tatgcttggc ggagatagac ctgaaaacct cagacctgta
     9001 ataaaatttg aaaaaaagga ctgggaaaga ttatcaaagc cagaattaat tgaatttgag
     9061 gatggtagaa aacatgtatc atggaatgta aatattatga gtgaaaaatt tgagattgct
     9121 ttctgttatc catacggaaa aaaagaaata gatgaaatta ttaaagaaag taagggttac
     9181 tggaaaaaag atgtgattgg aataagtcag gaagaaagag aaatgataag attaagtaat
     9241 agttatggag atattaaaaa gaagggggtt tatttaatgg caagacagca ttccggtgaa
     9301 acgcctggaa gttgggtttt agatggtttt ctgaggtatc tttccaaaat aaactataaa
     9361 aatttaattg tgtgggcagt tcctctttca aatatagatg ggattgaaaa tggttattat
     9421 ggtaaggata attttcctta tgatttaaat cgtgcatggg gatttcctcc tatgagacac
     9481 gaaacacttg taattcagag agatataatg agatggaagg aaagatgtat tccatttctt
     9541 gcaattgatt ttcatgctcc tggtgcatgt gaaatagatg gtgcatattt tttcattaca
     9601 ccttttgaaa aagataaaaa aatgtataaa attgagaaag agatgactga aaaaattgca
     9661 aaaaaagttg ggaaatatat gtctgaaaat tatatcaggt ctataaatta taagagcaga
     9721 tgggaaacac ctcatttttc tgattttata agaaataata ttaaaattcc ttctctttca
     9781 atggaaacac cgtattgcat ctgtcgtaat tgtttgatga aaaaagagga ctataaaaaa
     9841 ataggtaaaa aaattgctct tggtattatt gaggaaatag aaaaatatgg gacttaaaat
     9901 aggtacattt aaggaaaaac ttaaaataga agaaggaagt aaacttgcag gttatccgaa
     9961 tacagaaaca agattttgca aaggaagcaa aggagatatt gaagcaaatg gaataatttt
    10021 gaaaaatgat aaaaattttg gttgtttagt ttctcttgat attctcggga tagaacttcc
    10081 ctttactgaa aaaatcaggg agaaagttga aaaagaattt aaaatacctt ttgataatat
    10141 aatgatttgc tgtacacata ctcattcaag tgttatcaca gttggtagaa aatttggagc
    10201 agataaggag tttttaaatg aaattgaaag aaagatatta aagggtattg aagatggatt
    10261 taaaaacatt gaagaagtta atttaaaatg gacaaaaaca gaagttgata tttctcataa
    10321 tagaagagtt gttatagatg gaaaagcaaa aaatgaatgg caggatattg aaaaaaaaca
    10381 tacaggaata gttgatgaag aagtaatatg tataggattt ttcaaaaaga atggggattt
    10441 aaaaagttta attgttaatt atgcatgcca tccagttgtt ttaggtcctt caaattatta
    10501 tgcaagtgcc gattttgtgg gatatctgag aagtttcctt gaaaataatt taaatcttga
    10561 aaacctcgtt tatatcacag gagccagtgg aaatattaat ccgaggatat gtattaccga
    10621 taattttgaa gttgcgaaaa agacaggtga aactattgga gaacagattt taaagtgtaa
    10681 agaaaatttt cataatcttg aggtaaatga ttttcacttt gatagaaaaa aggtagtatt
    10741 tataaaaaaa gatggaacag gaattttaga gacagaaatg cagatattaa aaataggaga
    10801 aaaaatcatc tttattacat taccagggga acctgttgta gaaatagggc ttgatataaa
    10861 gaaaaaatca aaataccctg tcacaattgt tgcaggttat acaaacgatt atattggtta
    10921 tatctctaca gacaaaatac tctctgaagg aggacatgaa gcaaatagtt gtcctgtgaa
    10981 agaagttgaa aaaacaataa aagaaattgt aaagaattat atgaaaaatg gtagaactta
    11041 atacagcaat tgaaattaaa ttcatccatt taagttttga aacaggaatt gaagcacctc
    11101 attttcatga agaaaatcaa ttactttttt ttatctcagg caaagggatt gaaactgttg
    11161 aaaataaaaa atataaaata ttaccgggta ccttcatact tattccagaa aaaagggttc
    11221 atttttttaa atctacttta aataaacctg ctgaaatttt atctttgaga tttaaattgg
    11281 gtcagaaaat tgcagatgaa acatttactg atttttttaa aaaaacatat atttacagag
    11341 gggggaaaga aaatattaaa acaataaaaa aaatgatacc ctctataatt aatggtaaac
    11401 caaatttttc ttttctttta aaaattttga atatgatttt tgaaacctca actaaaactc
    11461 ctgaaaaaac aactgaatac atggaaaaat tagagaaata cattgaagaa aatataggga
    11521 gtgagattaa aataaagaaa actgcagaat atcttggaat ttcagaggtt tatgtgagaa
    11581 aacttgtaaa gaaaatttat gatataagtt ttacaaaatt tgtaaatatt aaaaaaattg
    11641 aatatgcttc taaattactt gttgagacaa aacttcctgt ttcaaaaata gccggaatgt
    11701 gtgggtttta tgattttaat tatttttcaa gagtttttaa aaaaattaaa agagtatcac
    11761 ctgttaaatt cagaaaaagt tatctttaaa gtggaggaag aatatggtca aaaaaatttt
    11821 tttaattctt tttatgttta ttttttgttt agaagcagaa acaatctggg ttgattgtag
    11881 ttccaagaag gataaagaaa cagggacaaa agaatttcct ttcaagaaaa tatcagatgc
    11941 aataaaaata tcaaaagaag gagatgagat tataattaaa caggggattt atagagaaac
    12001 tgttcaacca aaatcaggaa ttaaaataaa gggtgaggga agagttataa tttctggtta
    12061 tgaaaaaatt aaaggatggg aaaagtttga aaaaaatatc tggaaaacat atctgaacag
    12121 agaaattgat gaattattca tagaaaataa gagaattgaa attgcaagat atccagaaaa
    12181 aaactggttt aaagttgaga aatttgaaga aaaaggtgat atttacagaa tttataattc
    12241 tgaaataaaa aatttaaaag atgtgaaaaa ttttgaagta agaattctca attcaacagt
    12301 caacggtttt ttcacatgtc ctgtaataaa ttttaataaa gaagaaggtt tttttgaatt
    12361 tgcaaaacaa ccatatctga aattaactga aaaggattta ttttatcttc agcatagtcc
    12421 aaattttatt acttcacccg gtcagtgggc aattgaaaaa gaaaatgata gatatgttgt
    12481 ttatatttgg cctcaaaatg aaaaggaact gaattttatt gaaacaacgg gaaaagaaga
    12541 tagaatttta attttgagca gagtaaaaaa tgttgaaatt gatggaattg aaatatgtgg
    12601 aagtaataca aatggcttat ggattgataa cagtgaaaat ataaccatta aaaactgcat
    12661 tatatataac aataaaaatg ttggcttata tgttaacaga agcaaaaata caaaaataat
    12721 gaaaaatata gtacttttta atggtaatgg tatatcagtc ggaaattctt ctgatattga
    12781 aattttaaat aatgagattg ctttcaacaa tattgatggt ctgattgttt catggaattc
    12841 agaaaatgtt ttggtaaagg gaaactacat tcaccatcat attctttatg gacatcctga
    12901 taatatgcag acatacagaa atgtaaagaa tttaaaaatt gaagataatt tacttatttg
    12961 tggaggacag tccataatga tggaggaaac atatgatgtt gaattaaaaa ataatataat
    13021 tatgggggca ggtgcatact ctttaatttt tgggcatcag aatacagata atgtgaaaat
    13081 gtattttaat actgtagttc tttctcaata tggaagtatt agttttacag gaaaaaaata
    13141 tgatgttaag ggaaatatct ttattacagg tcatccaggt gctatttatg gaattaatgt
    13201 aaatgaatat gaaggtgatt ataatatttt ctggaattgt gaaatacaga atccaactgt
    13261 tattgcagca tcaggcaaat ggtataaatt aagtcaattt gaagtatata aaaaagatac
    13321 tcagaaagat ttaaattctt tatattccaa tccaatgttt aaaaattttc caaagtcctt
    13381 tcatattgtt gaaccaactc tatcagatga agaaaaaatt tgtatagtaa gtcagacaga
    13441 tttaataaat gaaggagatt ttgtagagat taattttgac ggaataccca gaaaagtgat
    13501 tgcaaaagaa ggaaaataca ttaaaatttc tccaaaactt gaaaaagtcc ctttcagaat
    13561 tgtgatggtc tgcaactggg gaagaaatga taattttgag tataattttg agtttaaaga
    13621 tgaaagccct gtaaaaaaag ttctttatga aaatcaaact cctggaagta aaataaattt
    13681 taagaatata aaaaattatg attttgatgg agacgggaaa agagatttac cgttgttatc
    13741 agaagacctg gataaagttt ataatgaaaa aatgaaaaaa acatatttag gaggtgaaat
    13801 agaaaagaaa tggaagtaaa aaatgaaaaa gatttattta tttttaatat tacttttaaa
    13861 aatttctttt tcagaggtat attatgtaag tatcaatgga agtgacgaaa accctggtac
    13921 aaaagaaaaa ccgttcagga caattcaaaa agcagcaaat gttgttaatc ccggtgatac
    13981 agttttagtt ttacccggtg aatataatga aaaagtgaat attttaaaaa gtggacaacc
    14041 tgaaaaatat attaaattta aaggagaagg aaaggttgaa gaaatcaaaa ccataagttt
    14101 tcaaattgga ggatgggctc caagaaaagc aaactacata attattgaaa acttttattt
    14161 atcaggagga gtgagtattt caggagatta taatattgtt cagaactgta ttttagaagg
    14221 ttatggagtt agcatttcat ttcatccaac ttctcatcct gattcaacag gttgtatagt
    14281 cagaaataat attatgagga attttggaaa ggttgtttta aattgtactg gaacaaaatc
    14341 aagtaattgt atttttgaaa ataatgttat ttataatgga gaaggagatg tgtggagaat
    14401 ttttggtaaa ggacatataa taaggaataa tgaagtttat gatttaagag aaaccggctg
    14461 gcatgctgac ttttttcaga tatatgataa caacagagaa atttcttatg atattcttgt
    14521 tgaaaataat tatttccatg actctacagg ttctattgct atgcttttaa gttatggata
    14581 ttcagattta agggactgga ctttcagaaa taatattttt tacaatatag gaggagttgc
    14641 acagattggt attccaggag ttaaattcta taacaatact tttgtaaact gtggaagaaa
    14701 tacagcagga ccgttacttt tcagatatta tgaaaaaaag ggtgaatttg aggtgggagg
    14761 tcatcgggca agaattttca ataacatttt tattggagga gggtcttatc ctgacagtga
    14821 cgaaattgga tggtatcatt ttgctgataa accaaaggaa gaaataaagg attttcaggc
    14881 agattttaat tatgtgacaa agacaaaaga tgcaaattat gctcctaaaa atgaattttc
    14941 aaaagttgaa aaaaatggaa taaatggagg agaccctaaa tttgtaaatt ttgaaaaaaa
    15001 ggattttcat cttcttccag atagtccagc aattgataga ggaactgaaa taaaggactg
    15061 gatagaccca aaagataaag atggcataaa aagaccatct ggttctaaat gggatatagg
    15121 agcatatgag tttgaaaaaa actaaaacca aacttataga ctttaaaatc aatataattc
    15181 tttgtgggat aatggattaa gtcaa
//
LOCUS       DAOSAT010000041        15092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2697_length_15092_cov_5.917470, whole genome shotgun sequence.
ACCESSION   DAOSAT010000041 DAOSAT010000000
VERSION     DAOSAT010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15092
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2697_length_15092_cov_5.917470"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..88)
                     /locus_tag="PLF90_04740"
     tRNA            complement(2..88)
                     /locus_tag="PLF90_04740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Ser,seq:gga)
     gene            complement(74..298)
                     /locus_tag="PLF90_04745"
     CDS             complement(74..298)
                     /locus_tag="PLF90_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56621.1"
                     /translation="MGKLKEELRKIYHRKREKALKQLKLLEEGKISYDQLNRLAKKIF
                     YKRINAGYQFPKTVFFKKVENKETSKGGVA"
     gene            449..1591
                     /locus_tag="PLF90_04750"
     CDS             449..1591
                     /locus_tag="PLF90_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK56622.1"
                     /translation="MTSKERMLYAIWNKKPDMVPVAPDMSNMIPAKLTGKPFWDIYFY
                     DNPPLWEAYIEALSYFKFDGWFIYGGIEMKYEKVIKEESRIIYRDNGKMEVEYFYYTP
                     EGVLHTIVVYPKDNPPWVKEHMIKNFKEDFKKIKYFSPSYYTSYKGFDDKKLKEMRDK
                     LGDKGVIGIGIPTPGFHIWQGAFEGGLLGLTYAYYDYPELFDQWAEIQHKDIINFTEY
                     VIKAKPDFILTGGSGAITLSSPSLFKKYSLPTIKVISEIANKEKIPVMIHCCGKEKDL
                     VDMCYNETLVNCINPLEEPPMGDCYLSEIKKKYGDKISLMGNINTTFMLTAKPEEIEK
                     VCKKAIDDAGENGGFILSTGDQLGRDTPEENIFKMIEVARTYGKYL"
     gene            complement(1598..1915)
                     /locus_tag="PLF90_04755"
     CDS             complement(1598..1915)
                     /locus_tag="PLF90_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOK56623.1"
                     /translation="MKKNEIKIYYYKSSGPGGQRKNKKLTSVKVLHIPTGMTAKGTEF
                     RSQAKNKEIALKRLEEKLNKLREKKKKRIKTEKPLYAKIKQIEEKRKRSEKKKLRQKI
                     KEF"
     gene            complement(1912..4143)
                     /locus_tag="PLF90_04760"
     CDS             complement(1912..4143)
                     /locus_tag="PLF90_04760"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOK56624.1"
                     /translation="MRYILKFVFAFLVFIFVFSLYKKNKFEKLEFIIYDSHIRNSTLK
                     YDLPLVIIGITGDFEKEIGEPFSRKHYSQILKILKEEKAELVVFDIFFPSFSEDKKED
                     VEFLNSIKENGRVILPVFSPIKLEKRVEEFYLVESLRGSAIQFERYAFSLGHINTFPD
                     KDQIVRRFPAYIKYGEKIYPQIGIETYRIKNKSTFLKILNSLPLDENKCFYIRYIPPN
                     FIDNYFISFSDVLKRKYPDGFFNKKVVIIGQTIVGAKNADLIPTPFGTQFGVFVQASA
                     IGTNLSGKYIKHINSVFFLFLYAFFLSLIFSIRNISLNTFNTLGFTGLCFFSSRFFLD
                     KYGIFFDVVPFLFFTIFYYLSFIFYSLFSTLKKLFQKEVILNLVKKTEEEFTEILNPI
                     ESFKKEDMFFLGFGSEDLIEKTPAMVLKTILIASGIECGCLVSFYHNKFEIIAKKGEL
                     IDKIDVEKILKNVEKAKIINKIKDENIKNMAIVPILYFPDFKVFGIFINKKPTVFSKT
                     SKFSYEDINLIQTISLPGIIAIQNSQLNLILKDAQLETIFRLAMSIEYRDRETGAHIH
                     RVSEYAYLIAEKLGFKKTECTLIKNAMPLHDIGKIAIPDNILLKPGILTKEERKIVEK
                     HPIIGAKMLEGSKSIVLKAAEVIALSHHEKYDGTGYPYGLSGNKIPIYGRIAALSDVF
                     DALTSKRIYKSSVNVERAFEIINEEKGKSFDPKLVEIFVNSRDEILEIHKKYIIEEIE
                     EEL"
     gene            complement(4150..5067)
                     /locus_tag="PLF90_04765"
     CDS             complement(4150..5067)
                     /locus_tag="PLF90_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOK56625.1"
                     /translation="MKKGFSLIETLILIFIGISVLILIVGVISNSREFARTMGCVNNM
                     KNIAQAIEGYQVDWKETPVTLSSLMPAYIQNPNVFHCPSDREKGDSYSKFYVGRFFAE
                     EDSNKVFLVCPRHFGGRRTVAGYLSYAVEIGKTKSVKWSGIDVEFGKTYIGGNLKFED
                     GTEVNEISGEIGVLGSFTNPENKIYSIIYVPEGDVPEGEKTSYKVEHKGDSRFEVITP
                     AVIAGVEGTKFNVVNSYYYNNGIPIDNTIISVFEGVVKVKERNQGRTETVNTGEEVLI
                     ETKTYGTKQKGVPRRPPKSVPHVIKRFVK"
     gene            complement(5051..5617)
                     /locus_tag="PLF90_04770"
     CDS             complement(5051..5617)
                     /locus_tag="PLF90_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56626.1"
                     /translation="MNIKKGRRILWVDTEIQLRFVIFTIITLAISCFIISSITFSNIW
                     VDISEKLLKIADVKELYSISVKQFILLNFALIILLAILATLGMIILSHKVAGPAYRIT
                     KILKELQEGKFPEFKLRRGDTLTSIIEELEKFSNQYKELSEAAINVIETWKNTEVKDI
                     SLNIALKELESKVSHISTNKEVKNEKRI"
     gene            5762..6241
                     /locus_tag="PLF90_04775"
     CDS             5762..6241
                     /locus_tag="PLF90_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOK56627.1"
                     /translation="MGKVLIIYHSRTGNTEEMAKSVAEGVKEEGVDVVLKPVEKTDCD
                     DLLDADGIIVGSPTYFGLPSAEIKKLFDDSVKFYGSLEGKVGGAFTSSAHIGGGNETT
                     IMGIIQMMLIHGMIVQGTTKSDHYGPVSIGKPNDKVKKDCKELGKRVAKLVKKIKGE"
     gene            6243..8975
                     /gene="ileS"
                     /locus_tag="PLF90_04780"
     CDS             6243..8975
                     /gene="ileS"
                     /locus_tag="PLF90_04780"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOK56628.1"
                     /translation="MDYSKTVYLPKTDFPMKANLSQREPEMLKLWEKIDIYKKMLEKN
                     REKKPYILHDGPPYANGHIHLGTALNKILKDIVVKYKSLSGFYTPFKPGWDCHGMPIE
                     HQVFKEIGKKKNEVDILDLRKKAEDYARKFYNIQKEEFKRLGVFAEWEKPYLTMYPEY
                     ESDIIRAFGKLYLSGYIYKTYKPIYWCITCETALADAEIEYSEKKSPSIFVKFKIIED
                     KGKKLGENSYFLIWTTTPWTLPGNTGIALHPDFKYILVDNENERLIIAEKRKEDVFKI
                     IGKELKILKEFNGKELEGIICKNPVIDRKSRVILAGFVSGEEGTGCVHTAPGHGEEDY
                     YAGIKNGLEVISPVNEKGEFTDEVSEFKGINVFKADKLIIEKLKKEGSLISSGEILHS
                     YPHCWRCKNPVIYRSTKQWFLKIDHNDLRKRMLEEINKVKWFPPEGINRIGSMVSQRP
                     DWCLSRQRLWGVFIPVFYCESCGKDILTEEILERICDLVKKFGSNVWIEKNNEELLPA
                     GFKCPYCSGEKFKKEMDILDVWFDSGVSHLAVLKEENGLIWPSDLYLEGSDQHRGWFQ
                     TSLITSCGIFGHPPYKTVLTHGFVVDGSGRKMSKSLGNVITPDEIIKKYGAEILRLWS
                     ISENYQEDIRISDEIVKNVVNGYRIIRNTIRFLLGNLNDYKKENEIPYENLLEVDKWA
                     IEKLKVVIKKITENYENFAFHKVFEEIYNFCNINLSSFYLDYLKDRLYTYPRNSVERK
                     AAQTVLSKILINLLILIAPILSFTAEEAYQFIPYDKKESIFLEEWPEIEEEDKNLLDR
                     WDKFFEIRKMVLKKLEEKREEKIIGSSLEAKVIIECDKDTSEFLKSFYKLQTLFIVSE
                     VEITNGENLNLKVEKTNNKKCQRCWVYFPEVGENKDFPDLCEKCIKAIKEDFLV"
     gene            8975..9481
                     /locus_tag="PLF90_04785"
     CDS             8975..9481
                     /locus_tag="PLF90_04785"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOK56629.1"
                     /translation="MEYIVDGFNVIKTSFIKKYEKYGIEGAQGFLIDILERYKKKHPS
                     VEITVVFDGKSPSISFYQKRRIKIIFSNEITADEKIRKILENKKNKTQIQVVSDDREI
                     QEFSKIIGSKFLKVDAFLEIVYPYEKKETVKKETVKKEKDINYRLKLEIEKELKNFYG
                     KKIKEFKR"
     gene            9450..10046
                     /locus_tag="PLF90_04790"
     CDS             9450..10046
                     /locus_tag="PLF90_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56630.1"
                     /translation="MEKKLKNLKDKILKFLEEEDRIREESIKVLREITRMATSSIKRI
                     HQEDLKNSKRELENCIKKLKEIKKKLKKYPEIYYQGFLHQAEKEVVEAIATYEIIVRK
                     NIPEMNDFDPISYLHGVCESLGEIRRYILDRMRKRKVMDIEDYLNIMDDIYYFILNFQ
                     YPDAITRSLRRQIDYARGIIEKTRGEITTYYLFKKKDR"
     gene            complement(10019..10768)
                     /gene="larB"
                     /locus_tag="PLF90_04795"
     CDS             complement(10019..10768)
                     /gene="larB"
                     /locus_tag="PLF90_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOK56631.1"
                     /translation="MDREKIKKLLIDFKEGKITEEKFIEKLKYFPYLDINFAKIDTHR
                     SLKFGFPEVVFGQNKSYQHLKEIISKIKKIHSNVIITRVEEKIANKLKKDFPEIIYFK
                     EAKILTFKKEKRKKDGYVCVVTAGTADIPVAEESAITLEILGIKTERIYDAGVAGLHR
                     LLDKIEILNKARCLIVVAGMEGALPSVVGGLVGKPVVAVPTSVGYGINLNGIAPLLTM
                     LNSCSPNVVVVNINNGFGAAFFTYLSFFLNK"
     gene            complement(10768..11622)
                     /locus_tag="PLF90_04800"
     CDS             complement(10768..11622)
                     /locus_tag="PLF90_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOK56632.1"
                     /translation="MANRIKTYFLLGILTVILVWIGGFFGREGLIIALIFAFFLNWFS
                     YFFSDKIVLAMYRAREIKENEEPYLHSLVAQLAQTAGIPKPKVYIINSSTPNAFATGR
                     NPGNAVVALTTGIMELLDENELKGVISHELTHIKNRDILVATVAATIAGAITFLARML
                     QYMAFFGSMDSDDRRGGNIFTLFALLLFAVLAPIAALIIQLAISRSREYLADEGGAKI
                     SGNPLYLANALRKLSYGVKRNPMRTANPATSHLFIVNPFSGSNLFALFSTHPPIEERI
                     KRLERMVF"
     gene            complement(11694..13751)
                     /locus_tag="PLF90_04805"
     CDS             complement(11694..13751)
                     /locus_tag="PLF90_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56633.1"
                     /translation="MKNLLLILKGVSGIFFQSIILREIFSSFFGNELSFSIVISFYLS
                     GGAIGSYLLRKNRNHSRNYILFTFFEIFFLIFFLIFLRIFSNIQRIFYISNLRFFIFS
                     FFLSFFSGFFEGARFILLSFLYKEEKSSGKVYGLEGLGFLIGGLLFSFLLFFKINIFF
                     LFFLSLILSFLTIFYFEKNYMYLSIFIIFLFLLPFSKKLDFKTNSLKYKGFKVVEVKD
                     TNYNKLTVLNKGNQYILLSNGFQEFTSQPDCFSIKNIAFFSLAFSEKIEKVGIYGSPE
                     ILDEIKKYDVSEIYFFEIDKEKTNLIKNYFLKDLKNIIFVNKDLNKFLKEKKITFDAF
                     IITNSQPMNLRDNYFLTDEFFRKIREFTQNLIIVLPGSYDYLGKGLLILHSSIYKTAK
                     KYFKYDIVAFTYPMIAVFSNGNLKLNKNFTFDRQFFNDEYLKFVLDENKKNYYLENIL
                     SFKTDLNTLSNQFCLFSSLSHYFSQTSQKVGRIISNIFFKIYSLRNSMLLLSLSLFLI
                     SLLPFTSTFSAIVFTNGFSCLTFEIIFIFLFQIYYGFVYGFISGIIGIFMAGISLGSL
                     LSVFKIHIRRIIYYTEIFHFLFYFLSVILIENGILSLFLIFFSGFLTGWEFGIVSYLI
                     KKESIVDTTGKLYSIDLLGALFSSLFLPLFLIPAFGISGCLWLIVLLKFSNLFRLFLK
                     RGV"
     gene            13781..14794
                     /locus_tag="PLF90_04810"
     CDS             13781..14794
                     /locus_tag="PLF90_04810"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF014752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HOK56634.1"
                     /translation="MEWIITKTDSVKFPYRLLIREREEIILSLLVQDKWPGVKNIFCY
                     RDNEIKEDEEIIERVEILSLRKYGERMVVILNRKSNKRCEFLFLKKKYKEKEGEYEQI
                     FWSTSKALKERKPKRGLLPYIKTELNIIIDKNERYPYKFQNANIERAILPCGDYALMD
                     GNRNIIAVVERKTFDNFLDSISNIHIFHQFLSELENYKNNALIIEANYSDFFKPQKIK
                     FYKVEFCARAISEIYSFHPNLKIIFTGNRKLAELWILRFFLNIINKIQEKPHIEIKEK
                     TSEYYTLRAITDEYLIKKFNNMPEKFKFSEAKSFFHEVTTNTLRNFLKKMEKKIYITD
                     YEK"
     gene            14784..>15092
                     /locus_tag="PLF90_04815"
     CDS             14784..>15092
                     /locus_tag="PLF90_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56635.1"
                     /translation="MKNKTKIIIFLTVFLVYLNSILNPFIFDDLRLIVENPFIKSFRF
                     FRFFFTKNLTEGVGEKSTFYRPIQLIVYSIIYKISGIFPINYHLLNILLHSGCAVLIY
                     L"
BASE COUNT         5735 a         2070 c         2167 g         5120 t
ORIGIN      
        1 gcggaggggg tgggattcga acccacgatc cgattgctcg gatacacgct ttccaagcgt
       61 gcgcactcgt ccactatgcg acccctcctt tgctggtttc tttattttca acttttttaa
      121 agaatacagt tttggggaac tggtatcctg catttattct tttataaaat attttttttg
      181 caagacgatt aagttggtca taactaattt taccctcttc aagaagtttt aattgcttta
      241 atgccttttc tctctttcta tgataaattt ttcttaattc ttccttgagt ttgcccattt
      301 ttacctcctt ctttcaaact tttaattttt ttattaacct tgactctttt attgaaaaat
      361 tatattagtt tttagaaatt ggtcaagatt aaattctcca aaaatgataa aatatacctt
      421 aaaagaaaat ttaaaatatg gagggattat gacttcaaaa gaaaggatgt tatatgcaat
      481 ctggaataaa aaacctgata tggtaccggt tgctcctgat atgtcaaata tgatacctgc
      541 taaactgaca ggaaaacctt tctgggatat atatttctat gataatccac ccttatggga
      601 agcatacatt gaagcacttt cttattttaa atttgatgga tggttcatat atggtggtat
      661 tgaaatgaaa tatgaaaaag ttataaaaga ggaaagtaga attatttaca gggacaatgg
      721 aaaaatggaa gttgaatatt tctattatac acctgaagga gttttgcata caattgttgt
      781 ttatccaaaa gacaatcctc catgggtgaa agaacatatg attaaaaact ttaaagagga
      841 ctttaaaaaa attaaatatt tttcaccatc atattacacc tcttataaag gttttgatga
      901 taaaaaattg aaagaaatga gagataaatt gggagataaa ggagttatcg gtatcggaat
      961 tccaacccca ggttttcata tatggcaggg tgcttttgaa ggagggctct taggacttac
     1021 ttatgcttat tatgactacc ctgaactttt tgaccagtgg gcagaaatac agcataaaga
     1081 cataataaat tttacagaat atgttataaa agcaaagcca gattttatac ttacaggtgg
     1141 ttcaggtgca ataactttat cttcaccctc tttatttaaa aaatattcat taccaacaat
     1201 taaagttata tctgaaatag caaataaaga aaaaattcct gttatgattc attgctgtgg
     1261 taaggaaaaa gaccttgttg atatgtgtta taatgaaaca cttgtaaact gtataaatcc
     1321 acttgaagaa cctccaatgg gtgactgcta tctttctgag ataaaaaaga aatatggaga
     1381 taaaatttct ttaatgggaa atatcaatac aacctttatg ctgactgcaa aaccagaaga
     1441 aattgaaaaa gtttgtaaaa aagcgataga tgatgctggt gaaaatggtg gtttcattct
     1501 ttcaacagga gaccaactcg gaagagatac acctgaagaa aatatattta aaatgattga
     1561 agttgcgaga acttatggta aatatctgta aagttaatta aaattctttt attttctgcc
     1621 ttaatttctt cttttcactt ctttttctct tttcttcaat ctgttttatt ttggcatata
     1681 acggtttttc tgtttttatt ctttttttct tcttttctct caatttatta agtttttctt
     1741 caagacgttt taaagcaatt tccttattct ttgcttgaga acgaaattca gttccttttg
     1801 cagtcattcc tgttggaata tgaagtactt taacactggt taatttttta tttttcctct
     1861 gccccccagg gccactactt ttataataat aaatttttat ttcgtttttt ttcataattc
     1921 ttcctctatt tcttcaatta tatatttttt atgtatttcc agtatttcat ctctggaatt
     1981 gacaaatatt tcaacaagtt ttgggtcaaa agatttaccc ttttcttcat ttattatctc
     2041 aaaagctctc tctacattta cagaactctt gtatattctc ttagaagtta aagcatcaaa
     2101 aacatctgat aatgcagcaa ttcttccata aattggaatt ttatttcctg ataaaccata
     2161 cggataccct gtgccatcat atttttcatg atgagataaa gcaattactt ctgctgcttt
     2221 taaaacaatt gattttgacc cctcaagcat ttttgctcca ataattggat gtttttcaac
     2281 tatttttctt tcttcttttg ttaatattcc cggttttaaa agaatattgt caggaattgc
     2341 tatttttcct atatcatgca aaggcatagc atttttaata agtgtacatt cagttttctt
     2401 gaaaccaagt ttttctgcta ttaaataagc atattcactc actctgtgaa tatgtgctcc
     2461 tgtttctctg tctctatatt caattgacat tgcaaggcga aaaattgttt caagttgagc
     2521 atctttcaaa atgaggttta attgagaatt ttgaattgct attatcccgg gcagtgatat
     2581 tgtttgaata agatttatat cttcatatga aaatttagat gtctttgaaa aaaccgtcgg
     2641 ttttttattt ataaaaatac caaaaacttt aaagtcagga aaatataaaa taggaacaat
     2701 tgccatattt tttatgtttt catctttgat tttatttatt atttttgcct tttctacatt
     2761 tttcaaaatt ttttcaacat ctatcttatc aatcaattct cctttttttg caattatttc
     2821 aaatttatta tgataaaagg aaacaagaca tccacattct attccagaag caatcaatat
     2881 tgtttttaat accattgctg gagttttttc tattaaatct tcacttccaa atccaagaaa
     2941 aaacatatct tcttttttaa atgattcaat aggattcaaa atttcagtaa attcttcttc
     3001 tgttttttta acaaggttta aaattacttc tttttgaaat agttttttta aagtagaaaa
     3061 taatgaataa aaaatgaagg aaagatagta aaaaatcgta aaaaataaaa atggaacaac
     3121 atcaaaaaaa attccatatt tatcaagaaa aaatcttgaa gaaaaaaagc ataacccagt
     3181 aaatccaaga gtattaaatg tattcaaaga aatattacga atcgaaaaaa ttaaagataa
     3241 aaagaaagca tagagaaaga gaaaaaaaac agaattgatg tgtttaatgt atttacctga
     3301 aaggtttgta cctattgcac ttgcctgtac aaaaacccca aactgtgttc caaaaggtgt
     3361 aggaattaaa tctgcatttt ttgcaccaac tattgtctgt cctataataa ctactttttt
     3421 attgaaaaaa ccatctggat attttctttt aaggacatca gaaaatgaaa tgaagtaatt
     3481 atcaataaaa tttggaggaa tatatcttat ataaaaacac ttattttcat caagtggaag
     3541 tgaatttaat atctttaaaa aagtgctttt attttttatt ctatatgtct caattccaat
     3601 ctgaggatat attttttccc catattttat ataagcagga aaccttctaa ctatctggtc
     3661 tttatcaggg aaagtattta tgtgtccaag agaaaaagca tatctttcaa attgaatagc
     3721 acttcctctt aaagattcaa caagataaaa ttcttcaact cttttttcaa gttttatcgg
     3781 tgaaaatact ggtaaaataa cccttccatt ttcctttata ctatttaaaa attctacatc
     3841 ttccttctta tcctctgaaa aagagggaaa aaatatatca aaaacaacaa gttctgcttt
     3901 ttcttcttta agtattttta aaatctgtga atagtgtttt cttgaaaagg gttctccaat
     3961 ttctttttca aaatcacctg taattccaat tataaccaaa ggtaaatcat actttagtgt
     4021 tgaatttcta atatgactgt cataaattat aaattcaagt ttttcaaatt tatttttttt
     4081 atataaagaa aatacaaaga taaaaaccaa aaaagcaaaa acgaacttta aaatatatct
     4141 catcaaaaat tacttaacaa accttttaat tacatggggc actgattttg gtggtcttct
     4201 tggcactcct ttctgttttg ttccataagt ttttgtttca attaaaactt cttctcctgt
     4261 atttactgtt tctgttcttc cctgatttct ttctttgact tttacaacac cttcaaaaac
     4321 agaaattatg gtattatcta tgggtattcc attattataa tagtatgaat taacaacatt
     4381 aaattttgta ccttcaacac ctgcaattac agcaggagta ataacctcaa atcttgaatc
     4441 tcctttatgc tcaactttat atgaagtttt ttctccttca ggaacatctc cttcaggaac
     4501 ataaattata ctataaattt tattctctgg atttgtaaaa gagccaagaa ctccaatctc
     4561 tccacttatt tcatttactt ctgtcccatc ttcaaatttt aaatttccac caatataagt
     4621 tttcccgaat tcaacatcaa taccagacca tttgacactt ttagttttcc caatttcaac
     4681 agcatatgaa agatatccag caactgttct tcttccaccg aaatgtcttg gacaaacaag
     4741 aaaaacttta tttgagtcct cttctgcaaa aaaccttcca acataaaatt ttgaataact
     4801 atcgcctttt tctctatcac ttggacaatg aaatacattg ggattctgaa tataagcagg
     4861 cattaaagaa cttaaagtaa caggtgtttc tttccagtca acctgataac cctcaattgc
     4921 ctgagcgata tttttcatat tattcacaca tcccatagtt cttgcaaatt ctcttgaatt
     4981 tgaaattaca ccaactatta aaatcaaaac acttatacca ataaaaatta aaattaatgt
     5041 ttcaattaaa ctaaatcctt ttttcatttt tcacctcctt attagtagaa atatgtgata
     5101 ctttactttc aagttctttc agagcaatat ttaaacttat atccttaacc tctgtgtttt
     5161 tccacgtctc tatcacattt attgctgctt cacttagttc tttatattga tttgaaaatt
     5221 tttcaagttc ctcaattatt gaagttaaag tatctcctct tcttaattta aattctggga
     5281 atttcccttc ctgcaactct ttcagtattt ttgttattct gtaagcaggt cctgctactt
     5341 tatgagaaag aattatcata ccaagagtag caagtatagc aagcaagata ataagagcaa
     5401 aattcaaaag tataaattgt tttacagaaa ttgaatataa ttctttaaca tctgcaattt
     5461 ttaaaagttt ttcagaaata tcaacccaga tattactgaa agttatactg cttataataa
     5521 aacatgaaat ggcaagagta ataattgtaa aaataacaaa ccttaattgt atttctgtat
     5581 ctacccacaa aattcttcta ccctttttta tattcatcat ttttatatta cctttatttt
     5641 ttttatttgt caagtaaaaa atttgttaaa ttcaaaaacc attactgtaa cttattttaa
     5701 aatttgacaa taatgattga aagttataga ttaaataaat atattttcaa ggagaaaaaa
     5761 tatgggaaaa gttttaatta tttatcattc aagaacaggt aatacagaag aaatggctaa
     5821 aagtgttgca gaaggagtta aagaagaagg agttgatgtt gtattaaaac cagttgaaaa
     5881 aactgactgt gatgatttac ttgatgcaga tggaataata gttggttcac ctacatattt
     5941 tggacttcct tctgctgaaa taaaaaaact ttttgatgat tctgttaaat tttatggttc
     6001 tcttgaagga aaagtaggag gggctttcac atcttctgct catattggtg gaggaaatga
     6061 aacaacaatt atgggcataa tacagatgat gcttattcat ggtatgattg ttcagggaac
     6121 tacaaaatca gaccattatg gacctgtttc tattggaaaa ccaaatgata aagttaaaaa
     6181 ggattgtaag gaactaggga aaagagtggc aaaacttgta aagaaaatca aaggagaata
     6241 agatggatta ttcaaagaca gtttatctac caaaaactga ttttcctatg aaggcaaatt
     6301 taagtcaaag agaaccagag atgttaaaat tatgggagaa aatagatatt tataaaaaaa
     6361 tgcttgaaaa aaatagagaa aaaaaaccct atatacttca tgatggacct ccttatgcaa
     6421 atggacatat acatcttggt actgcattaa ataaaatttt aaaggatata gttgttaaat
     6481 ataaatcatt aagcggtttt tatactcctt ttaagccggg atgggactgt catggtatgc
     6541 caatagaaca tcaagttttt aaggagatag gaaaaaagaa aaatgaggtt gacatacttg
     6601 atttaagaaa aaaggcagaa gattatgcaa gaaaatttta taatattcaa aaagaggaat
     6661 ttaaacgact tggagtattt gcagaatggg aaaaacctta tttaacaatg tatcctgaat
     6721 atgaaagtga tattataaga gcatttggca aactctatct ttccggatat atttataaaa
     6781 catataagcc aatttactgg tgtattacct gtgaaacagc acttgcagat gcagaaattg
     6841 aatattctga aaaaaaatca ccctcaatat ttgtaaaatt taagataatt gaggataaag
     6901 gaaaaaaatt aggagaaaat tcatactttt taatctggac aacaactcca tggacattac
     6961 cgggaaatac tggaattgcc cttcatcccg actttaaata tattcttgtt gataatgaaa
     7021 atgaaaggtt aataatagca gaaaaaagga aagaagatgt ttttaaaata attggtaaag
     7081 aattaaaaat tttaaaagaa ttcaatggga aagaacttga aggaataatc tgcaaaaatc
     7141 ctgttattga tagaaaatca agggtaattc ttgctggatt tgtttctgga gaagaaggaa
     7201 caggttgtgt tcatacagca cctgggcatg gagaagagga ttattatgca ggaattaaga
     7261 atggacttga agttatatct cctgtaaatg aaaagggaga atttactgat gaggtgagtg
     7321 aatttaaagg aataaatgtt tttaaagcag ataaattgat aatagaaaaa ttaaaaaaag
     7381 agggaagtct tatatcaagt ggagaaattc tgcattcata tccacattgc tggagatgta
     7441 aaaaccctgt catttataga agtacaaagc agtggttttt aaaaattgat cataatgatt
     7501 tgagaaaaag gatgcttgag gagataaata aggtaaaatg gtttcccccg gaagggataa
     7561 atagaattgg ttctatggtt tcacaaagac ctgactggtg tttatcaaga cagagattat
     7621 ggggtgtatt tattccagta ttttattgtg aaagttgcgg aaaagatata ttgacagagg
     7681 aaattctgga aaggatatgt gatctggtta aaaaatttgg ttctaatgta tggattgaaa
     7741 aaaataatga agaactttta cctgcaggat ttaaatgtcc ttattgttct ggggaaaaat
     7801 ttaaaaagga gatggatata cttgatgtat ggtttgattc aggagtaagt caccttgcag
     7861 ttttaaaaga agaaaatggt ttgatatggc caagtgattt atatcttgaa ggttcagacc
     7921 agcacagagg atggtttcag acctctttaa tcacttcatg tggaattttc ggtcatcccc
     7981 cttataaaac agttctaaca catggatttg tagtagatgg aagcggaaga aaaatgtcaa
     8041 agtcgcttgg gaatgttata acgcctgatg aaataattaa aaaatacgga gcagaaatac
     8101 ttcgtttatg gagcatatct gaaaattatc aggaagatat aagaatttct gatgagatag
     8161 ttaaaaatgt agtaaatgga tacaggatta taagaaatac aataagattt ctacttggta
     8221 atttaaatga ttataaaaaa gagaatgaaa taccttatga aaatctcctt gaagttgata
     8281 aatgggcaat tgaaaaattg aaagttgtga taaaaaaaat tacggaaaat tatgaaaact
     8341 ttgcttttca caaggttttt gaagaaattt ataatttctg taacattaat ctatcatctt
     8401 tttatcttga ttatctcaaa gaccgacttt atacataccc aagaaattca gtagaaagaa
     8461 aagcagcaca aacagtttta agcaaaatcc ttattaattt acttattctt attgctccta
     8521 tcctttcatt tactgcagaa gaggcatatc agttcattcc atatgataag aaagaaagta
     8581 ttttcctaga agaatggccg gaaatagagg aagaagataa aaacttactt gataggtggg
     8641 ataaattttt tgaaattaga aaaatggttc ttaaaaaact tgaagaaaaa agagaagaaa
     8701 aaattattgg aagttcactt gaagcaaaag taataataga atgtgataaa gatacatctg
     8761 aatttttaaa atcattttat aagttacaaa ccctctttat tgtttccgaa gttgaaatta
     8821 caaatggaga aaatttaaac ttaaaagttg aaaaaacaaa taataaaaaa tgtcagagat
     8881 gctgggttta ttttccagaa gttggtgaaa ataaagattt tccggactta tgtgaaaagt
     8941 gtataaaagc aattaaggag gattttctgg tttgatggaa tatatagttg atggatttaa
     9001 tgttataaaa acaagtttta taaaaaagta tgagaaatat ggaattgaag gggcccaggg
     9061 attcttaata gatatacttg aaagatataa aaaaaaacat ccctccgttg aaatcacagt
     9121 cgtttttgat ggaaagtcac caagtatttc tttttatcaa aaaagaagaa taaaaattat
     9181 tttttcaaat gaaataactg cagacgaaaa aataaggaaa atacttgaga acaaaaaaaa
     9241 taaaactcag attcaggttg tctcggatga tagagaaatc caggaatttt caaaaataat
     9301 tggaagtaag tttttaaaag tagatgcatt ccttgaaata gtttatccat acgaaaaaaa
     9361 agaaactgta aaaaaagaaa ctgtaaaaaa agaaaaagat ataaattaca gattaaaact
     9421 tgaaatagag aaagaactaa aaaactttta tggaaaaaaa attaaagaat ttaaaagata
     9481 agatactaaa atttcttgaa gaggaagata gaattagaga agaatcaata aaagttttaa
     9541 gagaaattac aagaatggca acatcctcta taaaaagaat acatcaggag gatttaaaaa
     9601 attcaaaaag agaacttgaa aattgtatta aaaaattgaa agagataaaa aagaaattaa
     9661 aaaaatatcc tgaaatttat tatcagggtt ttttacatca ggcagaaaaa gaggtagttg
     9721 aagcaatagc aacatatgaa atcatagtta gaaaaaatat acctgaaatg aatgattttg
     9781 acccaatcag ttatcttcac ggagtttgtg aatcacttgg agaaataaga agatatattc
     9841 ttgatagaat gagaaaaaga aaagttatgg atattgagga ttacttaaat attatggatg
     9901 atatttacta tttcatttta aattttcagt atcctgatgc aattacaagg tcgttaagaa
     9961 gacagattga ttatgcaaga gggattattg aaaaaacaag gggggaaatt acaacatact
    10021 atttatttaa aaagaaggat agataagtaa aaaaagcggc accgaaacca ttatttatat
    10081 taacaacaac cacatttggt gaacaactgt taagcattgt caaaagcgga gcaattccat
    10141 ttaaatttat tccatatcca actgaagtag ggactgcaac aacaggtttt ccaacaagcc
    10201 ctccaactac actcggtaga gcaccttcca ttcctgcaac aactatgaga catcttgctt
    10261 tgtttaaaat ctcaatttta tcaagtaatc tgtgaagtcc tgcaactcct gcatcataaa
    10321 ttctttcagt ttttattcca agaatttcaa gggtgattgc tgattcttca gcaactggta
    10381 tatcagcagt tccagcagta acaacacaaa cataaccatc tttttttctt ttttccttct
    10441 taaatgttaa aatttttgct tctttaaaat aaataatctc tggaaaatcc tttttcagtt
    10501 tatttgctat tttttcttca acccttgtaa taataacatt tgaatgaatt ttctttattt
    10561 ttgaaataat ctctttaagg tgctggtatg acttattctg tccaaaaaca acttctggaa
    10621 aaccaaattt taaagacctg tgggtatcaa tttttgcaaa atttatatcc agatagggaa
    10681 aatattttaa tttttctata aatttttctt ctgtgatttt tccctctttg aaatcaatca
    10741 gtaatttttt tatcttttct ctatccatta aaataccatt ctttcaagtc gtttaattct
    10801 ttcctctata ggtggatgtg ttgaaaatag agcaaataaa ttacttccag aaaaaggatt
    10861 aacaataaaa agatgacttg ttgcagggtt tgctgtcctc attggatttc tttttactcc
    10921 ataagacaat ttccttaaag catttgcaag atataaagga ttacctgaaa tttttgctcc
    10981 accttcatct gcaagatatt ctcttgaacg ggaaattgca agttgaatta ttaaagcagc
    11041 aattggtgct aaaactgcaa aaagcaaaag ggcaaaaagt gtaaaaatat tccctcccct
    11101 tctgtcatca ctatccattg aaccaaaaaa tgccatatat tgaagcatcc tcgcaagaaa
    11161 agttattgct cctgctatag ttgctgccac tgttgcaaca agtatatctc tgttttttat
    11221 atgtgttaat tcatgtgaaa taactccttt cagttcattt tcatcaagaa gttccattat
    11281 tcctgttgtt aaagcaacta ctgcattacc tggatttctt cctgttgcaa aagcatttgg
    11341 agttgatgaa tttatgatgt aaacttttgg ttttggaatc cctgctgttt gagcaagttg
    11401 tgcaactaaa gaatgtaaat atggttcttc attttctttt atttctcttg ctctatacat
    11461 tgcaagaaca attttatcgc tgaaaaaata agaaaaccag ttaagaaaaa aagcaaaaat
    11521 cagagcaatt ataagtcctt cccttccgaa aaaaccacca atccagacaa gaataacggt
    11581 taagatacct aataaaaagt atgtttttat tctattagcc atttttattt cctccttttc
    11641 ttctataatt atacagatgt tatatctcag tgtaaagttt caattcttcc gaactatacc
    11701 cctcttttta aaaataatct gaataaattt gagaatttta aaagaacgat taaccataaa
    11761 catccagaaa taccaaaagc agggattaaa aaaagaggta aaaataaaga agaaaataaa
    11821 gcacccaata aatcaattga ataaagtttt cctgtcgtat ccacaatact ttcttttttg
    11881 attaaatatg aaactatacc aaattcccaa ccagttaaaa atcctgaaaa gaaaattaaa
    11941 aacaaggata aaatcccatt ttcaatcagg ataacagata aaaaataaaa taaaaaatga
    12001 aaaatctctg tatagtaaat tattcttcta atatgaattt tgaaaacaga aagtaaactt
    12061 ccaagagaaa tacctgccat aaaaattcca attattccac ttataaaacc ataaacaaaa
    12121 ccataatata tctgaaaaag aaagataaaa ataatctcaa atgtgaggca ggagaaacca
    12181 tttgtaaaaa caatcgctga aaaggtagaa gtaaaaggca aaagggaaat taagaataaa
    12241 gataaagata aaagaagcat agaatttctt aaactataaa ttttgaaaaa aatattactg
    12301 ataattcttc ctactttttg tgatgtctgc gaaaagtaat gagataggga tgagaataag
    12361 cagaattgat ttgatagagt atttaaatct gttttaaaac ttaaaatatt ttccagataa
    12421 taattttttt tgttttcatc aaggacaaat ttgagatatt cgtcattgaa aaattgtcta
    12481 tcaaaagtaa aatttttatt caattttaaa tttccatttg agaaaacagc aatcattgga
    12541 taggtaaaag caacaatatc atatttaaaa tactttttcg ctgttttata aattgaagaa
    12601 tgaagaatta agagtccctt tccaagataa tcatatgaac ctggaagaac aataataaga
    12661 ttttgagtaa actctctaat ctttctgaaa aactcatctg tgagaaaata attatctctg
    12721 aggttcattg gttgagaatt tgttattata aaagcatcaa aagttatttt tttctctttt
    12781 agaaatttat ttaaatcttt atttacaaaa attatatttt ttaagtcctt taaaaaataa
    12841 tttttaatca gatttgtttt ttctttatct atttcaaaaa aataaatctc tgaaacatca
    12901 tatttcttaa tctcatcaag aatttcagga cttccataaa tcccaacttt ttctattttt
    12961 tctgaaaaag caagtgagaa aaaagcaata tttttaattg aaaaacaatc tggttgactt
    13021 gtaaattcct gaaaaccatt tgataaaaga atatactgat tccctttatt taaaactgtg
    13081 agtttattat aatttgtgtc cttaacctca accactttaa aacccttata tttcaatgag
    13141 tttgttttaa aatcaagttt ttttgaaaat ggtagaagaa aaagaaaaat aataaaaatt
    13201 gaaaggtaca tataattttt ttcaaaataa aagatagtta aaaaacttaa aattaatgaa
    13261 agaaaaaata agaaaaatat gttaattttg aaaaacaaaa gaaaagaaaa aagtagtcca
    13321 ccaattaaaa aaccaagtcc ttcaagtcca taaactttac ccgaactttt ttcttcttta
    13381 tagagaaagg ataaaagtat aaatctcgct ccttcaaaaa atcctgaaaa aaaagaaagg
    13441 aaaaaagaaa aaataaaaaa tcttaaattt gatatataaa aaattctctg aatatttgaa
    13501 aaaattctta aaaaaatcag aaaaaaaatc agaaaaaaaa tttcaaaaaa tgtgaaaagt
    13561 atatagtttc tgctgtgatt tctatttttc cttaacaaat aacttccaat tgctccacct
    13621 gaaagataaa aagaaattac aatactgaag gaaagttcat taccaaaaaa acttgaaaaa
    13681 atctctctta aaataattga ttgaaaaaaa attccagaaa caccttttaa aatcaaaagt
    13741 aagtttttca tttttttaat ttcacttata ataatataat atggaatgga taattacaaa
    13801 aacagattca gtaaaattcc cttatagatt gcttatcaga gaaagagaag aaatcatcct
    13861 ttctttatta gttcaggata aatggccagg tgttaaaaat atcttctgtt atagagataa
    13921 tgaaataaag gaagatgaag aaataattga aagagttgaa atattatctt tgagaaaata
    13981 tggagaaaga atggtggtta ttttgaatag aaaaagtaat aaaaggtgtg aatttttgtt
    14041 cttaaagaaa aaatataaag aaaaagaagg agaatatgaa cagattttct ggagtacatc
    14101 aaaagcactg aaagaaagaa aaccgaaaag aggtttgctt ccatatataa aaacagagtt
    14161 aaacataata atagataaaa acgaaagata tccttacaaa tttcaaaatg caaatataga
    14221 aagggcgatt ttaccatgtg gagattatgc tttaatggat ggaaatagaa acattatcgc
    14281 tgttgttgaa aggaaaacct ttgataactt ccttgattca atttcaaaca tacacatttt
    14341 tcatcaattt ttaagtgaac tggaaaatta taaaaataat gctcttatta ttgaagcaaa
    14401 ttattctgac ttttttaaac cacaaaaaat aaaattttat aaagtagaat tctgtgcaag
    14461 agcaatttct gaaatttatt catttcaccc gaatttaaaa atcatattta caggaaacag
    14521 aaaacttgca gaattatgga ttttaagatt ttttttaaat atcataaata aaatacaaga
    14581 aaaaccacat attgaaataa aagaaaagac atctgaatat tatactttaa gagcgattac
    14641 ggatgagtat ttaattaaaa aatttaataa tatgcctgaa aaatttaaat tttcagaagc
    14701 aaaaagtttt ttccatgaag ttacgaccaa tacattgaga aattttctta aaaaaatgga
    14761 aaagaaaata tatattacag attatgaaaa ataaaacaaa aataatcatt tttcttactg
    14821 tttttcttgt atatctgaat tctattttaa atccttttat ttttgacgat ttacgactta
    14881 tagttgaaaa tccctttata aaaagtttca gatttttcag attttttttc actaaaaatt
    14941 tgacggaagg agttggggaa aaatcaactt tttacagacc aatccagtta attgtttatt
    15001 ctattattta caaaatttct ggtatattcc caattaacta tcacttactt aatatacttc
    15061 ttcattcagg atgtgctgtt ttaatttatt ta
//
LOCUS       DAOSAT010000042        15038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2709_length_15038_cov_5.775879, whole genome shotgun sequence.
ACCESSION   DAOSAT010000042 DAOSAT010000000
VERSION     DAOSAT010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15038
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2709_length_15038_cov_5.775879"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLF90_04820"
     CDS             complement(<1..508)
                     /locus_tag="PLF90_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOK56636.1"
                     /translation="MAENKKVLVPIGPYHPLLEEPEYFQLYCDGEEVVDAEWIAGYNH
                     RGIEKLSESKHWDQVTFLVERICGICSTSHPIAYCNAVEDLLGIEIPERAKYIRNIIG
                     ELERIHSHLLWVGLAGHFLGYNTVFMWAWKYREGILDMFEMITGNRNHYAMLRIGGVR
                     RDIEDSDIP"
     gene            complement(495..935)
                     /locus_tag="PLF90_04825"
     CDS             complement(495..935)
                     /locus_tag="PLF90_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOK56637.1"
                     /translation="MIEKLKERLGERIKEIKVHNERRIYLTIDKKDLKEVANIIFNEM
                     KARYQIVTGIENFDSFEILYHFSFDREGGVIVSLKVFLEKEKPEIESLTDVIPGISYI
                     EREIWELLGINFLGHPNLKHFLLREDWQENLYPLRKGVKTDGRE"
     gene            complement(932..1348)
                     /locus_tag="PLF90_04830"
     CDS             complement(932..1348)
                     /locus_tag="PLF90_04830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOK56638.1"
                     /translation="MNLKLKALKKSLWVYHVSGGSCNNCDIEILDLLTPRFDVERFGI
                     ILAASPRHADVLLCTGIVNKKCVERIKEVYEQTPKPCVVVAIGACACSGGIFRDGYQM
                     AGPLNKIIPVDVYIPGCPPKPEAIIAGIVKFLKELK"
     gene            complement(1350..2279)
                     /locus_tag="PLF90_04835"
     CDS             complement(1350..2279)
                     /locus_tag="PLF90_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOK56639.1"
                     /translation="MSGIYYLGYFILSFISLSITGLFVSWIDRKLTARIQWRVGPPWY
                     QNFADLIKLSGKEVLIPEGGNKFIFVFSPILSVIASTLCGTILFLVLKKNFGFGGDLI
                     LFVYLLTIPSLSIILGGSSSGNVLASVGISRELKLLLSYELPFICGIIIPALKANSTT
                     SLVEIIKYQQINGSNILSLSGIIGFIIILIASTGKLGVVPFDLAEAETEIAGGALIEY
                     SGILLGFYKLSKAILFVVVPLFIVILYLGGTIITGFGSFLIFVVKYLVVLVLITLIRN
                     TNPRLRIDQTLRFFWGWLTLIGILGILISLSGV"
     gene            complement(2276..2767)
                     /locus_tag="PLF90_04840"
     CDS             complement(2276..2767)
                     /locus_tag="PLF90_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK56640.1"
                     /translation="MKKLFIDYDKCLECEKCDVKCSYFLHPENNGITPLREEIAFLLA
                     CRRCEDHPCINSCPTEALKREEGINKRSRFLCISCKSCMLACPFGTILPDIIPYITVK
                     CDLCTGRLKEDEIPLCVKTAKCEAIKFIDEKEVEGQEDIYKIGENLIVKCFSYIEKYG
                     LKK"
     gene            complement(2764..3594)
                     /locus_tag="PLF90_04845"
     CDS             complement(2764..3594)
                     /locus_tag="PLF90_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOK56641.1"
                     /translation="MEKNIYEPVKCEVIDNIKESPTINTVVLKPEREFSFLAGQFASL
                     TVPGIGEAPFTPSSSPFDKEKLEFTVMKTGRVTEKIHQLKKGDIVGVRGPYGKPYPLE
                     KFKGKEVLLVGGGVGLAPIRALFLALVETYKDYKKIVFCCGARTPQDYIYKELIFDKW
                     QKLCEKIPLCFRITVDKKDENWKYMEGVVTKTLENLDMDIKNGVAVVCGPPIMMKFTT
                     LKLLEIGFAPENIYLSMERKMYCAVGHCRHCMIGDLFVCKDGPVFTYDVLKDKPEIWA
                     "
     gene            complement(3597..4613)
                     /locus_tag="PLF90_04850"
     CDS             complement(3597..4613)
                     /locus_tag="PLF90_04850"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOK56642.1"
                     /translation="MEKLYIKVDDWKNFLKGKIKENRIFAPVENNNFLFYENLNEENL
                     EKIVYDKARAVEPVKIFLYPIKEFVVPEIKNIEKLVVMGVTSCDLRAIDVLDSVFKNS
                     DYKDPNFSKRRENILIISFDCKNPYNSCFCTLVGVKPYPEKNFDLNLSKIENGFIVEI
                     GSEKGKEFIGKDTRFYQVNESQIKQLEEDRKKVEEKINEINREFDIKNFENLKGMYHT
                     EYWQKPNDIKNCVSCGSCNFNCPTCVCFLLEDTSTDGNFKKVKVWDACLFPGYARMAS
                     GVSPRPTLFDRYANRLLCKYWYMKENFGIIGCTGCGRCISGCIGKIDKRRVLTEVLKE
                     KVKV"
     gene            complement(4695..5504)
                     /locus_tag="PLF90_04855"
     CDS             complement(4695..5504)
                     /locus_tag="PLF90_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK56643.1"
                     /translation="MVIYDIKNLSFSYGKNRVLKDINVEISEGEFVGIVGPNGSGKTT
                     FLKILSKILLPESGFIYYKGRDLKSIRSIDYAREIAYLPSEIEVTFNINVKDFLLLGR
                     FPFTGRFGKEKEEIIEKVGETLNIKKFFEKGINELSEGEKQRIYIAQIIIQNPKVILL
                     DEPTSHLDIGYQFQIMDILKRLNEKGITVISIFHDLNIASCYATKIILFKNGEIYRKG
                     NPEEVLTYQNIEDVYNTKVLVYKNPFTKKINVFGLPSYLLEGNRKNNSGNK"
     gene            complement(5498..6448)
                     /locus_tag="PLF90_04860"
     CDS             complement(5498..6448)
                     /locus_tag="PLF90_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOK56644.1"
                     /translation="MRKKLFLILFFIFSIYFGTFFGSKIYTFKDIFNLSEIEKTIFLK
                     IRIPRVICAFIVGAGLSISGAVLQSILKNPLAESYTLGISGGASLGIAIGVIMGKVYS
                     IPFFAFLGAIFSLFLIILLSIIRKFSTSSIILAGISLNFICSSLVLFLISISQYEKFY
                     STVLFLFGDLSSFSEKILIYAGILIFISFIFLFFSSRVYDILSIDEEKAKTLGINVNF
                     EKNVAFFITCLISSLCVSLSGIIGFVGLIIPHIVRAIYGSVHRKIIPISFLTGGGFLI
                     ICDTISKFIIRPVEIPVGVITGFFGGIFFLFLLFKERRIW"
     gene            complement(6445..6957)
                     /locus_tag="PLF90_04865"
     CDS             complement(6445..6957)
                     /locus_tag="PLF90_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOK56645.1"
                     /translation="MIEIYTGDGKGKTTAVIGLAIRARGHNLRVCYIYFHKNPEKWGY
                     GEFKILEKIGVDIFVFAEKHTCFENVDREELRNECLKGLDFIRKILKEKKYDVVICDE
                     ILIPLRDGFLKEEEIIEMMEEKPENIELVLTGRGATEKIIEKADYVSEIKKIKHPYDK
                     GIKARKGIEY"
     gene            complement(6950..7765)
                     /locus_tag="PLF90_04870"
     CDS             complement(6950..7765)
                     /locus_tag="PLF90_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOK56646.1"
                     /translation="MKKKLFLLIFSLYCFVSAEKIPERIVSLGPAITEDIFLLGKGDK
                     IIGNTIYCTRPEEAKYKEKVGNVIDVNIEKIYSLKPDIVFATNLTNPKDVKKLRDLGI
                     RVEIISYSLNFKQLCNDFIKIGKIIGKEKEAKEIVEKVKREIEEIKKKSKRKKPKVLI
                     QVGTRPLWVAGTDSFVNDIIEFTGGENVIKGKGGIYSMEEIIKLNPEIIIITTMGIDT
                     EEEKKNWKKYNMIDAVKNDKIFVVDSDKICSPTPLSFVEVVKELRRRTGFGND"
     gene            complement(7766..8494)
                     /locus_tag="PLF90_04875"
     CDS             complement(7766..8494)
                     /locus_tag="PLF90_04875"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOK56647.1"
                     /translation="MREKINKRKGFTLIELLVVVAIIGILSAMLMPALAKARERARQA
                     TCLSNLKQLTTAFLMYVQDNDDYFPPTYYLTTTSEIAWDFATDTWWATYRPGIIGSYL
                     TKGVFECPTAKKIIKSYDRPFTGYAYNASYIGGGYSVWDGQATPPTKLSRIRKPSETV
                     LLCDSASWSSFTNEIIGNNYLRAPNDPYYSWIGPNVHFRHNGFANVAFCDGHVESLRT
                     KYNVSSKNSSLGDLSKDDSLYDLE"
     regulatory      complement(8661..8866)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8980..9052)
                     /locus_tag="PLF90_04880"
     tRNA            complement(8980..9052)
                     /locus_tag="PLF90_04880"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9017..9019),aa:Lys,seq:ttt)
     gene            complement(9333..10277)
                     /locus_tag="PLF90_04885"
     CDS             complement(9333..10277)
                     /locus_tag="PLF90_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOK56648.1"
                     /translation="MKSEILLFNGKRIENEDLNFNDLRRGFLYGDGIFETLISINKKI
                     FRFDQHWERMKKGAFICNLRMPDKKRIKKILSDNLEDGFYYIRLNLWRKRPDIFSPLK
                     KKDSNFLIIIKKFRPYPERFYKEGIKCTISKEIRRNEKSVLSKIKSINFLENVILKIE
                     AEKEKCDDIVVLDNVGYISEGSIANIFFVKNDIIYTPSLECGCLEGITRKIVFEICKK
                     EKIKIKEGFFKPDFLKNCDEVFFTNTLMGIMPVREIKGYFKSKKFKYSEYFGKLYWKI
                     LKCAFVLSYFFLFLLPVFIFSTNFIFCWKKFVYNFKIS"
     gene            complement(10252..11652)
                     /gene="pabB"
                     /locus_tag="PLF90_04890"
     CDS             complement(10252..11652)
                     /gene="pabB"
                     /locus_tag="PLF90_04890"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOK56649.1"
                     /translation="MKIFVENFPYINPEKFYLFICEYPFSFFMEVNWNKIEKYTICGF
                     SPFLIFRSKKRNIEIKRYNQIKRLKNDPISELQKIFDSYKLSSEYFFLPGGFGFLSYD
                     LGWQIEDLPDISEDDLNLPDIFICFYEMILVFDNYRKKAILISSNLEKDRHFNKKFVQ
                     IKKFLKKNIEKAKNLRNENPEFKIEKLSQNMSFSRYISSIEKIKNYIKEGDVYQINFS
                     HRFEVKGEFRPYPLFLKLKKINPAPYSAYFNFDDFILISNSPELFLKKQGREIVTKPM
                     KGTRRRLNDKKLDEKIKNDLLESKKDRAELIMIVDLERNDFGKICEYGSVKVKKLINL
                     EKYKTVYQTTSTIEGKLRKDVNLEKIIKAIFPGGSITGAPKKRAMEIIEELEPTKRSF
                     YTGSSGYFGFNENLQLNILIRTLLLKNNKIYYPCGGGIVWDSIPEKEYEETIVKARNL
                     FLTIGIKEDEIRDFVI"
     gene            complement(11636..12085)
                     /gene="rpiB"
                     /locus_tag="PLF90_04895"
     CDS             complement(11636..12085)
                     /gene="rpiB"
                     /locus_tag="PLF90_04895"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOK56650.1"
                     /translation="MKIGIASDHAGFELKNYLIENLKKEGCEVVDYGPEVKKVVDYPD
                     YAEKVAKSILNKEIEYGILICGTGIGMDIVANKFKGIRAARCCSIYDAILARQHNNAN
                     ILTLGGRLIGKELAFEIAKTFISTPFTKGRHLKRVNKIKRIENENFC"
     gene            12218..13927
                     /locus_tag="PLF90_04900"
     CDS             12218..13927
                     /locus_tag="PLF90_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOK56651.1"
                     /translation="MIPREEHPRPDFKRKEWINLNGSWDFEIDLSNTGIEKKYYLPEK
                     KFKEKIIVPFPPESKLSGIENKDFMVSVWYKRKIAIPENWRNKRIFLNFGAVDFLTNV
                     WINGEKIGTHIGGYTPFRFEITEFLKSENILVVNAYDDNRNFLQPCGKQSQKYNSYGC
                     HYTRVTGIWQTVYLEGTGKNYIKNFRIYPDYDTGRVLFLVEIEGMGNLKVEVYAENEK
                     SVEKEAYGSKFIFLETDIKDFIPWSFENPFLYDVRFSLYSDGKKEDEVCSYFGFRKIE
                     IRDKKIFLNKKPIFMRMVLDQGYYPDGIYTAPDENSIINDIKIAKDMGFNGARLHQKI
                     FEPIFLYWADKLGYLIWGEYPNWGIKNGINHECFGIFVKEWIEKIERDFNHPSIIGWC
                     PLNETSPSQDNELVRTLYRITKNIDKTRPVIDTSGYVHVETDIYDCHNYKQNPEEFKK
                     DFEPFKKNDTIWQNFPQFDAPYKGQPYWVSEYGGIWWDVEGKKNGWGYGERPKTENDF
                     IERYRKLTEILLNHPKICGFCYTQLYDVEQEVNGLYTYSRKPKFNPEIIRKINTQKAE
                     IEK"
     gene            14084..14503
                     /locus_tag="PLF90_04905"
     CDS             14084..14503
                     /locus_tag="PLF90_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOK56652.1"
                     /translation="MKSYTEYIWFCTEKKVEFINITEKVEETVRKSGIKEGLCVVNAM
                     HITASVFINDDERGLHSDFLDFLEKLAPHQPVSKYRHNLTGETNADAHLKRTIMGREV
                     VIAITDGKLDLGPWEQIFYGEFDGQRKKRVLIKIIGE"
     gene            14507..14908
                     /locus_tag="PLF90_04910"
     CDS             14507..14908
                     /locus_tag="PLF90_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOK56653.1"
                     /translation="MENLFLEPKEISEKLVKIGIKKAEGNIFELFFFGIFAGIYIGFG
                     AQCALTIMSGGTLDPRFTKFLAGSVFSVGLMLVLIPGAELFTGNLLMTIGLFERKYNF
                     LKLLRNWLIVYLGNFLGSIFLAYIFENRSLW"
BASE COUNT         5158 a         2604 c         1842 g         5434 t
ORIGIN      
        1 taggtatatc gctatcctct atatctcttc ttacacctcc aattcttaac attgcatagt
       61 gatttctatt tccagttatc atttcaaaca tatcaagtat tccttctctg tatttccatg
      121 cccacataaa aacagtatta tatccaagaa aatggcctgc aagtcctacc caaagtagat
      181 gactatgaat tctttcaagt tcaccaatta tattccttat atattttgcc ctttcaggta
      241 tttcaattcc aagtaggtcc tcaacagcat tacaataagc aataggatga gaagttgagc
      301 atattccaca tattctttca acaaggaaag ttacttggtc ccagtgtttt gattcagaaa
      361 gtttttcaat tcctctatgg ttatatccag caatccattc agcatcaaca acctcttctc
      421 catcacaata aagttgaaaa tattctggct cttctaaaag tggatgatat ggaccaattg
      481 gaactaaaac ctttttattc tctgccatct gtttttactc ctttcctcaa tgggtataaa
      541 ttttcttgcc agtcctctct taataaaaaa tgttttaaat ttggatgccc aagaaaattt
      601 attccaagca attcccatat ttctctttct atatatgaaa tcccaggtat aacatctgtc
      661 aaactttcaa tttcaggctt ttctttttca aggaatactt ttaaagaaac aatcactcca
      721 ccttctctat caaaagaaaa atggtacaga atttcaaaac tatcaaaatt ttcaatacct
      781 gttactatct gatatcttgc tttcatttca ttaaaaatta tatttgcaac ttctttcaaa
      841 tcttttttgt caatagtcaa atagattctt ctttcattat gaacctttat ctctttaatc
      901 ctttctccta atctttcttt caatttttct atcatttcaa ctccttcaaa aatttaacta
      961 ttcctgctat tattgcctct ggttttggtg gacatcctgg gatataaaca tcaactggaa
     1021 taattttatt taaaggacct gccatctggt acccatccct aaaaatccca cctgagcatg
     1081 cacaagcacc aattgcaaca acaacacatg gttttggtgt ctgctcgtaa acttccttaa
     1141 tcctttcaac acatttttta ttcacaattc cagtacataa taaaacatca gcatgtcttg
     1201 gagaagcagc gagtataatc ccaaaccttt caacatcaaa tctcggagtt aaaaggtcaa
     1261 gaatttcaat atcacaattg ttacaacttc ctccactcac atgatatacc cataaacttt
     1321 tcttcaatgc tttaagtttt aagttcatat taaactccac ttaatgaaat cagtataccc
     1381 agaattccta taagagttaa ccatccccag aaaaatctca atgtctggtc tattctcaat
     1441 cttggatttg tatttcttat caatgttata agtacaagca caactaaata tttaactaca
     1501 aaaattaaaa aacttccaaa acctgttatt atagttccac caagataaag aatgactata
     1561 aataaaggaa caacaacaaa taaaattgcc tttgaaagtt tataaaatcc aagtaaaatt
     1621 ccagaatatt caattaaagc acctcctgca atctctgttt ctgcttcagc aaggtcaaat
     1681 ggaacaacac caagttttcc agttgaagca attaaaataa taataaaacc aataatccct
     1741 gatagagaca aaatattact tccatttatc tgttgatact taattatttc tacaagtgaa
     1801 gtagttgagt ttgcctttaa agcaggtatt attattccac atataaaagg aagttcataa
     1861 gatagaagta attttaattc cctacttata ccaacagatg caaggacatt tcctgatgaa
     1921 cttccaccaa gaattataga aagagaaggg attgtcagta aataaacaaa taaaatcaaa
     1981 tctcctccaa atccaaaatt ttttttaaga acaagaaaga gtattgttcc acataaagtt
     2041 gaggcaatga cagaaagaat tggagaaaaa acaaagataa atttattacc tccttcaggt
     2101 attaagacct cttttcctga aagttttata agatcagcaa aattctgata ccatggaggt
     2161 ccaactctcc actgaattct tgctgtaagt tttctatcta tccatgaaac aaaaagtcca
     2221 gttatactta aactaatgaa agataaaata aaataaccca aataataaat tccactcatt
     2281 ttttcaatcc atatttttca atataactga aacacttaac aatcagattt tctcctattt
     2341 tataaatatc ttcctgtcct tcaacctctt tctcatcaat aaatttaatt gcttcacatt
     2401 ttgctgtttt aacacataaa ggtatttcgt cttcttttaa tcttccagta cacaaatcac
     2461 atttcactgt aatatatgga ataatatcag gtaaaattgt tccaaaagga caggcaagca
     2521 tacaactttt acaggaaata cataaaaatc tgctcctttt gttaattcct tcttctcttt
     2581 taagtgcttc tgttggacat gaatttatac atggatggtc ttcacatctt ctacatgcaa
     2641 gtaaaaaagc aatctcttct cttaaaggag taattccatt attttcagga tgtaaaaaat
     2701 aactacattt tacatcacat ttctcacatt caagacactt gtcataatca ataaataact
     2761 ttttcatgcc catatctccg gtttatcctt caatacatca tatgtaaaaa caggcccatc
     2821 tttacataca aataaatcac ctatcataca gtgtctgcaa tgaccgactg cacaatacat
     2881 ttttctttcc attgataaat aaatattttc aggtgcaaat ccaatttcaa gtaatttcaa
     2941 tgttgtaaac ttcatcatta ttggtggacc gcaaacaaca gccaccccat tttttatatc
     3001 catatcaaga ttttcaagtg tttttgtaac gactccttcc atatatttcc agttctcatc
     3061 tttcttgtca actgtaattc tgaaacagag aggtattttt tcgcataact tctgccattt
     3121 atcaaaaatt agttctttat atatataatc ctgtggagtc cttgctccac agcaaaaaac
     3181 tatcttttta taatccttat atgtttcaac aagagcaaga aataatgccc ttataggagc
     3241 aagaccaaca ccaccaccaa caagtaaaac ttctttacct ttaaattttt caagtggata
     3301 tggttttcca taaggacctc taactccaac tatatctcct ttttttaact ggtgtatttt
     3361 ttctgtaacc cttcctgtct tcataactgt aaattcaagt ttttctttat caaatggaga
     3421 agaagaaggt gtaaatggtg cttctccaat tcccggaact gttaaagaag caaattgccc
     3481 tgcaagaaaa gaaaattctc tttctggttt taaaacaaca gtatttattg taggtgattc
     3541 ctttatatta tcaattactt cacatttaac cggttcatat atattttttt ccatctttaa
     3601 acctttactt tctcctttaa aacctctgtc agaacccttc ttttatcaat tttccctata
     3661 catccactta tacaccttcc acaaccggta catccaataa ttccaaaatt ttccttcata
     3721 taccagtatt tacataacaa tctatttgca tatctatcaa acaaagttgg tcttggactt
     3781 actcctgaag ccattcttgc atatccggga aaaagacagg catcccagac cttaactttt
     3841 ttaaaattac catctgtact tgtatcctca agtaaaaaac aaacacatgt cggacagtta
     3901 aaattacaac tgccacaaga aacacaattt tttatatcat ttggtttttg ccagtattct
     3961 gtatgataca ttccttttaa attctcaaaa tttttaatat caaattctct gtttatttca
     4021 tttatctttt cttctacttt tttcctatct tcttccaatt gttttatctg tgactcattt
     4081 acctgataaa atcttgtatc ttttcctata aattccttac ctttttcact tccaatttca
     4141 acaataaagc cattttcaat tttactcaaa tttaaatcaa agtttttttc aggatatggc
     4201 tttactccaa caagtgtaca gaaacaacta ttatatggat ttttacagtc aaaacttata
     4261 attaagatgt tttctcttct ttttgaaaaa ttcgggtctt tataatcact atttttaaaa
     4321 acagaatcaa gaacatcaat agcccttaaa tcacaggaag ttactcccat aacaacaagt
     4381 ttttcaatat tttttatttc aggtacaaca aactctttaa ttggatacag aaatatttta
     4441 acaggttcaa ccgcccttgc tttatcataa acaatcttct ctaaattttc ttcattcaga
     4501 ttctcataaa ataaaaaatt gttattttca acaggtgcaa aaatcctatt ttcttttatt
     4561 tttcccttta aaaaattctt ccagtcatcc acttttatat ataatttttc cattttattt
     4621 gtaatttttt tcacaaactg ttttataaat tttacacaaa aaaattttta agtcaaattt
     4681 tttgtatgaa acttctattt atttccagaa ttattttttc tatttccttc aagtaaatat
     4741 gaaggtaacc caaagacatt gatttttttt gtaaatggat ttttgtaaac aagaactttt
     4801 gtattataaa catcttcaat attttgatat gtcaagacct cttctggatt gccttttctg
     4861 taaatctctc catttttgaa taaaattatt tttgtggcat agcaggaagc aatattcaaa
     4921 tcatgaaaaa tacttataac tgtaatccct ttttcattta gtcttttaag aatatccata
     4981 atctgaaatt ggtatccaat atcaagatga ctcgttggtt catcaagtaa tattactttt
     5041 ggattctgga ttattatctg tgcaatataa atcctctgtt tttctccttc cgataattca
     5101 ttgattcctt tttcaaaaaa ttttttgata tttaatgtct ctccaacttt ttcaattatt
     5161 tcctcttttt ccttaccgaa tctaccagta aatggaaatc taccaagaag caaaaaatcc
     5221 tttacattga tattaaatgt aacttcaatt tcactcggta agtaagcaat ttctcttgca
     5281 tagtcaatac ttctgatact ttttaaatcc ctccctttat aataaataaa accgctttca
     5341 ggcagtaaaa tttttgataa tatttttaaa aaagttgttt ttccacttcc attcggtcca
     5401 actataccaa caaattctcc ttctgaaatt tcaacattta tatctttcaa aactctattt
     5461 ttaccataac tgaaactcaa atttttaata tcataaatta ccatatcctt ctttccttaa
     5521 ataaaaggaa taaaaagaaa ataccaccaa aaaatccagt tataacacca acaggaattt
     5581 ctacaggtct tattataaat tttgaaatag tatcgcagat tataagaaaa ccgccaccag
     5641 ttaaaaaaga aatgggaatt atttttctat gtactgaacc ataaattgcc cttacaatat
     5701 gaggaatgat taaaccaaca aaaccaatta ttccagaaag tgatacacat aaagaagaga
     5761 taagacaggt tataaaaaat gcaacatttt tttcaaagtt tacatttatt ccaagtgtct
     5821 ttgccttttc ctcatctata cttaaaatat cataaactct tgatgaaaag aagagaaaga
     5881 taaatgaaat gaaaattaga ataccagcat aaattaaaat tttttctgaa aaagaggata
     5941 aatcaccaaa taaaaataaa acagttgaat agaatttttc atattgtgaa attgaaatta
     6001 aaaataaaac aagtgaagaa cagataaaat ttaaagaaat cccagcaagt attattgatg
     6061 atgttgaaaa ttttcttatt attgacagta aaataattaa aaatagtgaa aaaattgcac
     6121 ccaaaaaagc aaaaaatgga attgaatata ctttacccat tattacacct attgctatgc
     6181 caagagaagc accacctgaa attccaagag tataactttc agcaagagga tttttaagaa
     6241 ttgattgtaa aacagcacct gaaattgaaa ggccagcacc aactataaat gcacatataa
     6301 ctcttggaat tcttattttt aaaaaaattg ttttttcaat ttcagaaaga ttaaaaatat
     6361 ccttaaatgt ataaattttt gagccaaaga aagtcccgaa ataaattgaa aaaatgaaaa
     6421 ataaaatgag aaataatttt tttctcaata ttcaatcccc ttccttgctt ttattccctt
     6481 atcatatgga tgttttattt tttttatttc actcacataa tctgcctttt caataatctt
     6541 ttctgttgcc cctctacctg ttaaaacaag ttcaatattt tctggttttt cttccatcat
     6601 ttcaattatt tcctcttcct ttaaaaatcc atcccttaaa ggaatcaaaa tctcatcaca
     6661 tataacaaca tcatattttt tctctttcag aatttttctt ataaaatcaa gcccttttaa
     6721 acattcattc ctcaattctt ctctatccac attttcaaaa caggtatgtt tttcagcaaa
     6781 aacaaaaata tcaactccaa ttttttcaag tattttaaac tctccataac cccatttttc
     6841 tggattttta tggaaatata tataacaaac ccttaaatta tggccccttg cccttatcgc
     6901 aagtccaatt accgctgttg tctttccttt tccatctcct gtataaattt caatcatttc
     6961 cgaaccccgt cctcctcctt aattctttaa caacttcaac aaaagataat ggagtgggac
     7021 tgcaaatctt atcactatca actacaaaaa ttttatcatt ttttaccgca tctatcatat
     7081 tgtatttctt ccagtttttc ttttcttcct ctgtatctat tcccatagtt gttataatga
     7141 ttatttcagg atttaatttt attatctctt ccatactgta aatacctcct tttcccttta
     7201 taacattttc tccacccgta aactcaatta tatcattaac aaaagaatca gtccctgcaa
     7261 cccataatgg tcttgttcca acctgaatta gtacctttgg cttttttctt tttgattttt
     7321 tcttaatctc ttctatttct ctttttactt tttccacaat ttcttttgct tctttttctt
     7381 ttcctataat cttccctatt tttataaagt cattacaaag ttgcttgaaa tttaaagaat
     7441 aacttataat ttcaactctt attcctaaat ctctcaattt tttcacatcc tttggatttg
     7501 ttaagtttgt tgcaaatact atatcaggct ttaatgaata aatcttttca atattaacat
     7561 caattacatt acccactttt tccttgtatt ttgcttcttc tggtcttgtg cagtatatag
     7621 tatttccaat aattttatcc cctttgccaa gtaaaaaaat atcttcagta atagcaggtc
     7681 caagagaaac aatcctttca ggaatttttt cagcagaaac aaaacagtat aaagaaaaaa
     7741 ttaataaaaa taactttttt ttcatttatt caaggtcata taaactatca tcttttgata
     7801 aatctccaag agaagaattt tttgaactca cattatattt tgttcttaaa ctttcaacat
     7861 gtccatcgca gaaagcaaca tttgcaaatc cattatgtct gaaatggaca tttggcccaa
     7921 tccatgaata ataagggtca tttggtgctc ttaaatagtt atttcctatt atttcatttg
     7981 taaaagagga ccatgaagca ctatcacata atagaactgt ttcagatggt tttcttattc
     8041 ttgataattt tgttggtgga gttgcctgac catcccatac agaataccct cctccaatat
     8101 atgaagcatt ataagcataa cctgtaaatg gtctatcata tgactttatt attttttttg
     8161 cagttgggca ttcaaataca ccttttgtta aataacttcc aataatacct ggtctataag
     8221 ttgcccacca tgtatctgtt gcaaaatccc atgcaatttc agatgttgtt gtaagatagt
     8281 aggtaggagg gaagtaatca tcattatcct gaacatacat taaaaaagca gttgttaatt
     8341 gttttaaatt actcaaacag gttgcctgtc ttgctctttc ccttgctttt gcaagtgctg
     8401 gcataagcat tgctgaaaga attcctatga tagcaactac aacaagcaat tctatcaatg
     8461 taaacccttt tcttttatta attttttctc tcatttgtcc ctcctttttt taaatatttt
     8521 tcaaaacttt tacttttttt caaaatcttt cttcaattta tttcctttta tatcacctcc
     8581 tctaaataaa aaagcccgcc actttttttg gcgggctgca cccgttttcc tttgccattt
     8641 ctttccgccc tcgcagaaag aaatatcgcc atatccgggg caggtcttct gactcgggct
     8701 tcatcctact cggcagcctt cccatccaaa tttatagaca gtggcttttt cgcctttcgt
     8761 ctgccctcac agctccgggg gagtggctga tcatccccca attattttgg cggactacag
     8821 cacttcccta ttaagccata aaggcacccc gaattttcaa ttttaaaata cacctaaaaa
     8881 ataaatttgt caagtccttg ttatgtttga aattattaaa aaaagagagc ccccgggtag
     8941 ggagctctcc gaaagggggt gaggggggat gtgggtttgt aggccgcgca ggactcgaac
     9001 ctgctgcccc cgccttaaaa gggcggtgct ctgccgggtg agctagcggc ccagccggtt
     9061 aaaaaaatta aagaaaatga ttatactttt attttaactt tatgtcaatt tattttataa
     9121 aacccctttt cttctaaatg atttaaagtt accttaaata cactacacaa atttttttat
     9181 ataaaatttt accgcatatt ttattttatg taaaatcttc gaattgcctc attaaaaaaa
     9241 tctagttgaa attatacctt atctcctgga aaataaaaag aaaaaaggaa aaaaagatgt
     9301 aagataaaaa aaattaaatt aaaaaataaa gtttacgaaa ttttgaagtt atatacgaac
     9361 tttttccagc aaaagataaa attggtagag aaaataaaaa caggtagtaa aaaaaggaaa
     9421 aaatatgata agacaaaggc acattttaaa atcttccagt ataatttccc aaaatattct
     9481 gaatatttaa atttttttga tttaaaatat cctttaattt ctctgacagg cataattccc
     9541 attaaagtat ttgtaaaaaa aacttcatca caatttttta aaaaatctgg tttaaaaaat
     9601 ccttccttta ttttaatttt ttccttttta catatttcaa aaacaatttt tcttgttatc
     9661 ccttcaagac atccgcattc aagtgaagga gtgtaaataa tatcattttt aacaaaaaaa
     9721 atatttgcga tacttccttc agaaatatat ccaacgttat caagaactac tatatcatca
     9781 catttttctt tctctgcctc tatttttaat atcacatttt caagaaaatt tattgatttt
     9841 atttttgata atacagattt ttcatttctt cttatttctt tgcttatagt acattttatt
     9901 ccctctttat aaaatctctc cggatatggt ctaaattttt ttataattat taaaaaatta
     9961 gaatcctttt ttttcagagg agaaaatata tctggtcttt tacgccacag gttcaatctt
    10021 atatagtaaa aaccatcttc cagattatct gataatattt tttttatcct ttttttatca
    10081 ggcattctca aattacatat aaatgctcct tttttcattc tctcccagtg ctggtcaaat
    10141 ctgaaaattt ttttatttat agaaattaat gtctcaaaaa tcccatctcc atataaaaat
    10201 ccccttctta agtcattaaa atttaaatct tcattctcta ttcttttccc attaaataac
    10261 aaaatctctg atttcatctt cctttatccc tattgttaaa aataaatttc ttgcctttac
    10321 tattgtttcc tcatattctt tttcaggtat actatcccag acaattcctc ctccgcaggg
    10381 ataataaatt ttattatttt tcaaaagtaa agtccttata agaatattta gttgtaaatt
    10441 ttcattaaaa ccaaagtatc cggaagaccc tgtataaaaa cttctttttg ttggttcaag
    10501 ttcttctata atttccattg ctcttttttt gggagcacct gtaatagaac ccccaggaaa
    10561 aatcgctttt ataatttttt caagatttac atcttttctt aatttacctt ctattgttga
    10621 agttgtttga taaactgttt tatatttttc aagatttata agttttttta ctttaacact
    10681 tccatattca cagatttttc caaaatcatt tctttcaagg tctactatca taataagttc
    10741 tgctctatcc tttttacttt caagtaaatc attcttaatt ttttcatcta attttttatc
    10801 attcaatctt cttcttgttc ctttcatcgg ttttgttaca atttctcttc cctgtttttt
    10861 taaaaataat tcaggggaat ttgaaatcaa aataaaatca tcaaaattaa aataagcaga
    10921 ataaggagca ggatttatct tttttagttt taaaaataaa ggatatggtc taaattctcc
    10981 ttttacttca aatctatgtg aaaaatttat ctggtaaaca tctccttcct taatataatt
    11041 ttttattttt tcaattgagg aaatatatct tgaaaaactc atattttgac tcaatttttc
    11101 tattttgaac tccggatttt catttcttaa attttttgct ttttcaatat tttttttcaa
    11161 gaatttcttt atctgtacaa atttcttatt gaaatgcctg tctttttcca aatttgatga
    11221 aattaaaatt gccttttttc tgtaattgtc aaaaaccaaa atcatctcat aaaaacatat
    11281 aaaaatatca ggcaaattta aatcatcttc tgatatatca ggcaaatctt ctatctgcca
    11341 tccaaggtca taagataaaa atccaaaacc acccggtaga aaaaaatact ctgaacttaa
    11401 tttataagaa tcaaaaatct tctgaagttc agaaattggg tcatttttca acctttttat
    11461 ctgattatat ctttttattt caatatttct ttttttgctt ctaaaaatta aaaagggaga
    11521 aaaaccacag attgtatatt tttcaatttt attccagtta acttccataa aaaaactgaa
    11581 aggatattca cagataaaaa gatagaattt ttctggattt atgtaaggaa agttttcaac
    11641 aaaaattttc attttcaatt cttttaattt tattcactct tttcaggtgt cttccttttg
    11701 taaatggagt tgatatgaat gtttttgcaa tttcaaatgc aagttctttc cctatcaatc
    11761 ttcctccaag agttaatata tttgcattat tgtgctgcct tgctaaaatt gcatcatata
    11821 ttgaacaaca tcttgctgcc cttatacctt taaatttatt agcaactata tccataccta
    11881 ttcctgttcc acatatcaaa attccatatt caatttcttt attcaggata gattttgcaa
    11941 ccttttcagc ataatcagga taatcaacaa ctttttttac ttcaggtcca taatcaacaa
    12001 cttcacaacc ttcttttttc aaattctcaa ttaaataatt tttcaattca aaacctgcat
    12061 ggtctgaagc aattccaatt ttcatctttt tcctttttgt tttattataa acttttttaa
    12121 atgaatgtaa aatttctttc ttctgaaatt aataaaaaag tttttgacag aaaattgaaa
    12181 agagatatta ttttttaagg caaagaagga ggactaaatg ataccgagag aagaacatcc
    12241 aagaccggat tttaaaagaa aagaatggat aaatttaaat ggaagttggg attttgaaat
    12301 agacctatca aatacaggta ttgagaaaaa atattatcta cctgaaaaaa aatttaaaga
    12361 aaaaataatt gttccttttc ctcctgaaag taaactttca ggaattgaaa acaaggactt
    12421 tatggtatct gtatggtata aaagaaaaat tgcaatccct gaaaactgga gaaataaaag
    12481 gatattttta aattttggtg cggttgattt tcttacaaat gtttggataa atggagaaaa
    12541 aattggaacc catataggtg ggtatactcc ttttagattt gaaataacag aatttttaaa
    12601 aagtgaaaat attcttgttg tcaatgccta tgatgacaac agaaattttt tacaaccctg
    12661 tggaaaacag tcccaaaagt ataactccta tggatgtcat tacacaagag taactggaat
    12721 atggcagaca gtttatcttg aaggtacagg taagaactat attaaaaatt ttagaattta
    12781 tcctgattat gatacaggaa gagttttatt tttagttgaa attgaaggaa tgggtaattt
    12841 gaaggttgaa gtatatgctg aaaatgaaaa atctgtggaa aaagaggcgt atggaagcaa
    12901 attcattttt ttagagacag atattaaaga ttttatacca tggagttttg aaaatccttt
    12961 tctttatgat gtcagattct ctctttattc agatggtaaa aaagaggatg aggtatgttc
    13021 atattttggt ttcagaaaga ttgagataag agataaaaaa atttttctta ataaaaaacc
    13081 gatttttatg aggatggttc ttgaccaggg gtattatcct gatggaatct atacagcacc
    13141 tgatgaaaac tcaattataa atgatataaa aatagcaaag gatatgggat ttaatggagc
    13201 aagattacat cagaaaattt ttgaaccaat attcttatac tgggctgata aacttggata
    13261 tcttatatgg ggagagtatc cgaactgggg aataaaaaat ggaattaatc atgaatgttt
    13321 tggaattttt gtaaaggaat ggatagaaaa aattgaaaga gatttcaatc atccttcaat
    13381 aatcggttgg tgtcctttga atgaaacaag tccatctcag gataacgaac ttgtaagaac
    13441 tttatatagg attacgaaaa atattgataa aacaagacct gttatagata cttctggata
    13501 tgttcatgtt gaaacagata tttatgactg ccacaactat aaacagaatc cagaagagtt
    13561 taaaaaagat tttgagccat ttaaaaaaaa tgatactatc tggcagaatt ttccacaatt
    13621 tgatgctcct tataaaggac agccatactg ggtaagtgaa tatggaggga tatggtggga
    13681 tgttgaagga aaaaagaatg gatggggata tggagaaaga ccaaaaacag aaaatgactt
    13741 tattgaaaga tacagaaaac ttactgaaat acttttaaat catcctaaaa tatgtggttt
    13801 ctgctatact caactttatg atgtagaaca ggaagttaat ggactttaca catattcaag
    13861 aaaaccaaaa ttcaacccgg aaataataag aaaaataaat actcaaaaag cagaaataga
    13921 aaaataatat ggaggacgtt ctgcaaaggg ttagcaccct tgggcatggg tctaaaaaac
    13981 caatttatat acgcagtaac gtcctccatt tttaattata cctttaagtt tcaaaaagta
    14041 aacagatatt gaaaaaaaga aaagagatag taaaataaaa gtaatgaaaa gttatactga
    14101 atacatctgg ttctgcacag aaaagaaagt tgagtttata aatataacag aaaaagttga
    14161 agaaacagtc agaaaaagcg gaattaaaga aggattatgt gttgtaaatg caatgcatat
    14221 aaccgcaagt gtttttataa atgatgatga aagggggttg cattctgact ttcttgactt
    14281 tcttgaaaaa cttgcaccac accagccagt ttcaaaatac agacataatc tgacaggtga
    14341 aacaaatgca gacgctcatt taaaaagaac aataatggga agagaagttg ttattgcaat
    14401 tacagatgga aaacttgatt taggtccatg ggaacagatt ttttatggtg aatttgatgg
    14461 acagagaaaa aaaagagttt taataaaaat aataggggaa taatatatgg aaaatctatt
    14521 tttagaacca aaagaaatat ctgaaaaact tgtaaaaata gggattaaaa aagcagaagg
    14581 aaacatcttt gaacttttct tttttggaat ctttgcaggt atatatattg gttttggtgc
    14641 tcaatgtgca ttaacaatta tgagtggtgg aactcttgac cctagattta ctaaatttct
    14701 tgcaggaagt gttttcagtg ttggacttat gcttgtttta atcccgggtg ccgaactttt
    14761 tactggaaat cttttaatga caataggact ttttgaaaga aaatataatt ttttaaaact
    14821 tttaagaaac tggttgattg tttatctggg gaatttttta gggtctatat tcttagcata
    14881 tatttttgaa aacaggtctt tgtggtaatt cttcaacagg tttaacatct attggagaac
    14941 ttgctgtaaa agtagcagaa acaaaaataa atttgagttt tactgaagca ttttttagag
    15001 ggattctgtg taatatgctt gtttgtcttg cgattata
//
LOCUS       DAOSAT010000043        14601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2798_length_14601_cov_4.962326, whole genome shotgun sequence.
ACCESSION   DAOSAT010000043 DAOSAT010000000
VERSION     DAOSAT010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14601
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2798_length_14601_cov_4.962326"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..86)
                     /locus_tag="PLF90_04915"
     tRNA            complement(1..86)
                     /locus_tag="PLF90_04915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Leu,seq:caa)
     gene            183..1544
                     /gene="thrC"
                     /locus_tag="PLF90_04920"
     CDS             183..1544
                     /gene="thrC"
                     /locus_tag="PLF90_04920"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOK56654.1"
                     /translation="MEKILYRSTNRASEVVDFKYAVLKGQAPDYGLYMPVKIPFLKKT
                     EIDLFKGKSYSQIAFSALSKFLENEIPSEKINEIVERAYNFEVPLEKINENLYILYLD
                     RGPTASFKDFAARFMAEIMQYLADEENLNLTVLVATSGDTGGAIASAFFDKENIKVVV
                     LFPEREITEVQRKQMTTLNKNIIPIALKGKFDDCQSLVKMAFNDPELKNLNLTSANSI
                     NISRLLPQSVYYFYAFSKLNFEKIVFSVPSGNFGNLMGGVIAKKMGLPVEKFIVAVNE
                     NDEFPKFLETGEYKPVIPSRKCNSNAMNVGHPSNLARLIDLYGGWIYDERSIDGKVIK
                     HGVLKEKPDMKKMKEDFVSFSIKDEEVKETIKKYYQKYKIILDPHGAVGIAAFEKSNI
                     DEPVISIETAHPGKFPEVIKEVLGIEPEMPETLKNLISKEEKFIVLENNYNIFKKFLK
                     DLK"
     gene            1541..2746
                     /locus_tag="PLF90_04925"
     CDS             1541..2746
                     /locus_tag="PLF90_04925"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOK56655.1"
                     /translation="MKYIVMIMDGAADLPVEELNNKTPLQVAKKPNIDYITKLGCSGM
                     LKTIPDNFITDSGVANLSILGYNPKNVYTGRGVLEAYANGIELKENEIAFRCNLITVK
                     EGKIESYSAGYISTEESKELIDFLNKKLGSEKVKFYPGLGFKNILILKNDFSEDIKCY
                     PPHDYYGYDIEKIMIEGKTEKDKKTAEFLNNLIIESNKYLENHPVNLKRAKEGKEKAN
                     YIWPWSPGKKPNLEKFYDKFKIKGAVISAVDLIKGIGKMIGFEVINVPGATGLWNTNY
                     EGKAEYAIEALKKFDFVYVHVEGIDEAGHEGNLELKIKCIEDFDKRLVKIILENIDLK
                     STSIAILPDHYTPVKLKSHQVGYVPFVIYSPLNKPDEVEKFDEESCKNGKIGYIEGEK
                     FINSFLKGL"
     gene            2749..3381
                     /locus_tag="PLF90_04930"
     CDS             2749..3381
                     /locus_tag="PLF90_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOK56656.1"
                     /translation="MYIIIVGCGKIGSYLAKLFDEKNDVVVIDKDEKSFEKLIDFNGI
                     TKLGDALDIDVLKEAGIEKADAIAVITSNDNANIVIGQVAKKMFKVPKVIIRISDPNK
                     EKICKLLDIETINTTTLIASLIKEGLTKKIYSTHLIENEDFAIVELKNENFVGKKIEE
                     INVNGELQIFAIIRGNKGIVPDKNFKIEKDDIIIGITERKNLNKFKRILK"
     gene            3381..4049
                     /locus_tag="PLF90_04935"
     CDS             3381..4049
                     /locus_tag="PLF90_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOK56657.1"
                     /translation="MNIIIVGGGNVGYYLSEKLSDNHYVVLIEKETKISEKIAEKLNC
                     LVITGDGCDPEVLKNAGIKKADCLAAVTGSDEDNLVICQIAKEIFNVKRVVARVNNPK
                     NEKTFYQLGVDVAISGTSLIAKIIEEEVNWEDFITLFTFKKGKLSILRIDLPEKSPVL
                     GKKINEINLPDDSVIVAVMRGDDLFIPKSDFTFKENDEVIAITKVENENLLLNSLIGE
                     VKEV"
     gene            4195..5694
                     /locus_tag="PLF90_04940"
     CDS             4195..5694
                     /locus_tag="PLF90_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOK56658.1"
                     /translation="MIPQFSRDDLKGIFHYTGKLIILIGYLLLFPLFIAILYKEWNPA
                     LDFIFTSLFSLSIGYLIVSQFPESSDINWRQGMTIASLIWLIYMFLGAIPLYLSGHYK
                     SYMDSCFEAMSALATTGLVLVNDLDHMAHSYNFWRHFMCFIGGQGIILLGLLIFFKGA
                     SAFKIYVGEAREEKILPNVVQTSKFIWIVSTCYLIVFTLILGFVNILHGFSPSRGFFH
                     AICLFMAGWDTAGFAVQTQNIVYYHSGLVDLITALIVILGATSFALHYAVWTGKFKEI
                     FENYEFKVFLISIISLTFLCFAGFLKSVKNIDFISGFRIVFYQIISGHTGVGYTNIDP
                     YHFKTYWNDFSLIILTIAMGLGGCSCSTSGGIKILRIGLIFKEIKKEIKGYSYPFSTV
                     IIEKYHHIKDISLSDVQIKTVAIISIMYLVTYLIGGIIGTFLGYPFIHSLFESTSACA
                     NVGLSVGITNPDMPGILKITYIIEMWIGRLEFISIFVFIKYLISLRSPE"
     gene            5691..6173
                     /locus_tag="PLF90_04945"
     CDS             5691..6173
                     /locus_tag="PLF90_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOK56659.1"
                     /translation="MKKLIFILLFSTLIYSSEKVKIKELFENSKKYNGKSVIIEGEAI
                     GEIMGRGKEKWVNIKDVFEDFAIGVVVSEKDAKIIENLGSYKVKGDILRIEGIYNVNC
                     LKHQGERDIHAVRVEIVKKGERYKEEIILKKVILCFLLLIITSFLMTFAHKKEISAKE
                     "
     gene            complement(6179..7486)
                     /gene="miaB"
                     /locus_tag="PLF90_04950"
     CDS             complement(6179..7486)
                     /gene="miaB"
                     /locus_tag="PLF90_04950"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOK56660.1"
                     /translation="MSKKFFIKTYGCQMNFYDSERLRALLKRENFIEVSIPEEADYII
                     VNTCSVRKHAEDRAISFLSSLKNFKNNKKFCLVGCTPSLYREEIFKRYDFVDIICGPN
                     SYKKFVEVLKNNFYDKKIGIFQEDMDLFNEILLPEIRGSSVSSFISITKGCENFCSYC
                     VVPYTRGKLISKPVGIILEEIKKLVEKGIKEVILLGQNVNEYGKDNGENFVELLTKVH
                     NIDGLLKIGFLTSHPKDISTDLINLFSELPKLYKHLHLPLQSGSNKILKLMNRKYSLE
                     KYIEIVQRARNACPDISITSDIILGFPYEDEKDFEQTYRVIEEIEFDDLYVFKYSPRP
                     LTEANKFPDNVPKEEKEKRHRLILNLQDKISLLKNKKKEGKIDDVFIKKESYKKKNSY
                     IGRTTNNKPVIVETEEKKLEGKIFKVKIEKGERHYLYGSIVRV"
     gene            complement(7497..7754)
                     /locus_tag="PLF90_04955"
     CDS             complement(7497..7754)
                     /locus_tag="PLF90_04955"
                     /inference="COORDINATES: protein motif:HMM:NF025572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HOK56661.1"
                     /translation="MKIFYVFSVLFLCAMIGSCIGEIILMFIPQGTIYYNFLSNHLSP
                     VWEIKQLDLIIFNLSFSIRFNINSFTLLGLIIGSIFSLKKV"
     gene            7901..7974
                     /locus_tag="PLF90_04960"
     tRNA            7901..7974
                     /locus_tag="PLF90_04960"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7935..7937,aa:Arg,seq:tct)
     gene            7987..8059
                     /locus_tag="PLF90_04965"
     tRNA            7987..8059
                     /locus_tag="PLF90_04965"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8020..8022,aa:Lys,seq:ctt)
     gene            complement(8218..9183)
                     /locus_tag="PLF90_04970"
     CDS             complement(8218..9183)
                     /locus_tag="PLF90_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOK56662.1"
                     /translation="MKDEDYQEKGAEIVNDHAYIFKNSDLIIKVKEILPSEYNLLCEG
                     QMIFTFFHFSSNIEMTEKLIEKKLSCIAYELVEEEGILTILKPMSEIAGKLSVQEGMK
                     YLEKEYGGKGVLLSGAEGVKPGKVVILGGGTVGYNAAKIAYSIGAKVVIFEIDKEKIK
                     RLKREFPKGKILYSEKEKIKKEIKDADVVIGAVLIPGRKAPVLIKEEDLKIMEEGTVI
                     VDVSIDEGGVFETSHPTTHKNPIYKYEGIVHYCVPNIPGIVPKSSTYALCNVTEKYIL
                     QLADKGLKAIEISQPLKSGLAIKYGEIVNQKLSWAMQDLAIPSTK"
     gene            complement(9313..10617)
                     /locus_tag="PLF90_04975"
     CDS             complement(9313..10617)
                     /locus_tag="PLF90_04975"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOK56663.1"
                     /translation="MKKKKISDFRYDTGPARIPWSAVGEKIKLQDIMEILKFLIPPKN
                     KKYKIQLKKVEKELKKLIENSELSSKLTLGDKVRTLEEEVKKFLKCKYAVFLTNATAG
                     FEIAYKFAGLKPGDEVIAPAITFIATISYPLSIGAKVVLVDVDPKTLNMDPEDLARKI
                     TKNTKVIVPVHIGGYPCDMDAIMDIARKFNAVVLEDAAHAFGAKYNDKMVGTIGHFGA
                     FSFHEVKNITSLGEGGILVTNEKIGEDFPKARFVGFNISNPIPFWLYDVVALKWRGDY
                     FAPGNHSATEIQAVALLSQMKRINKIIGKRRKAAEYLNERFKNVKGIVIPPLDDKKIK
                     STHHLYLFQVDPGVLNGDIQKVKEKMGEKGITQIPHFAPLYRFSIMKQLGYDTEKIKE
                     TCPNAEEVFLHRFTHLPLYDFKKVDLKYMSDTIIKIIKEMQK"
     gene            complement(10665..11807)
                     /locus_tag="PLF90_04980"
     CDS             complement(10665..11807)
                     /locus_tag="PLF90_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOK56664.1"
                     /translation="MVSKYTVYRKNILKLIYQNENISRKNLHDLLNLRFATITHITRD
                     LIKEGIIEESGKRKRKSKGRPHELLKIIPNSKYFIGCELTPEKIISVILNFKGEFIGQ
                     KTVNLDETDKKEEILIKIVNLLKNLIEESKIEKNKICGIGFVDPGIVDMQKGVSVFSS
                     ILPGWENVHIRDYLKQKLSINTLIIGSSQSKVLSEKFLGKGRKSKNIIFIEYGKGIAC
                     GIINDNKIIHGQGGVAGEFGHVKIPYKDEICNCGKRGCLESIISVPAILKKIKDVKGT
                     ELKMNEIIERYEKGDSEIKKFMDDVFNILVFSVSNLINILNPEIVIFDKNFLVFEKEF
                     KNIFEKIKENMVYEYPVSFEISDFGEEIGAIGGACLVISNFLGLDI"
     gene            11911..13005
                     /locus_tag="PLF90_04985"
     CDS             11911..13005
                     /locus_tag="PLF90_04985"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOK56665.1"
                     /translation="MGKEVIILECKDYSVDLIYEKLKKGIELLKINKIPEKILIKPNM
                     LSARQPEEGVTTHPAIISALIEIFKNKKIIIGDSPAGISKPIEEYWEKCGYKEIGDKY
                     KIELKKFENSFPLDICVNNKVYKIPVTSLLNDFSILNAPKFKTHNLTTITVGVKNLYG
                     LIPGIIKSYLHSKFITPYEFSFFLIEFYKKIEEKIFLTVVDGITGMEGDGPSAGNLRN
                     IGYLIIGEKPIYVDYACCKLIGIKPEKVDFIEIYMDKYGIEKPEIIGDFKEIKNFQLP
                     STKKYFFIRNKLLSKFILPIAKFFKIAPVIDKKKCKKCFTCYNICPVKAISNDLKFNR
                     KKCILCLCCFEVCPYKAIEIKKSFIARIFT"
     gene            13019..14233
                     /locus_tag="PLF90_04990"
     CDS             13019..14233
                     /locus_tag="PLF90_04990"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOK56666.1"
                     /translation="MDYEKILSEIIQKNDKKILLLVLDGVGGLPHPETGKTELETANI
                     PNLDYFAETGSCGLIIPVLPGITPGSGPGHIALFGYDPIEVQIGRGILECLGIGLEVK
                     KGEIAIRGNFATIDKNRIVIDRRAGRIPTEENEKIVKLISENIKEIKGVKFKIQTVKE
                     HRCALILSGENLSCDVTENDPGKEGLPLKKFEPLGEKGKFIAEILNEFEEKVISILGS
                     LNSKAKCLLLRGFSTYPDIQTFKERYKLNAVCIATYPMYRGISKLVGMDVIDCGETID
                     SEIETLKKVYNDYDFFFLHIKKTDSFGEDGNFEGKVKILEEIDKKIEPIKNMNFEAIG
                     ITGDHSTPSLLKSHSWHPVPFVLISPNTLPDDVKRFTERDCAKGILGNIYSKNVMYLL
                     LACSLKLAKFGA"
     gene            14235..>14601
                     /locus_tag="PLF90_04995"
     CDS             14235..>14601
                     /locus_tag="PLF90_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HOK56667.1"
                     /translation="MFRGGIKLIDFKSLSKNEEIEEFKYPERVIIPLSQHTGSPAKPV
                     VKKGDYVLEGQIIGEITGFISSYIHSSISGKVVDILPWDLPTNRKSLSIVIESDGERK
                     SIERQVRDWLKLESNEIINI"
BASE COUNT         5333 a         2013 c         2138 g         5117 t
ORIGIN      
        1 tgccggagga gggatttgaa ccctcacgga gtttgggctc caccggattt tgagtccggc
       61 gcgtctgcca gttccaccac tccggctttg atatatttag attatacttt ccattaaaaa
      121 aaaatcaact atttcacctg atattgactt ttttcaaaaa aacggtaata tcatataaaa
      181 ttatggaaaa gattttatat agaagtacaa atagagcatc agaagttgtt gattttaaat
      241 atgctgtttt aaaaggacag gcacctgatt acggacttta tatgcctgtt aaaatccctt
      301 ttttaaaaaa gacagaaata gatttattca aaggaaaaag ttattctcaa atcgcattct
      361 ctgccctttc taaatttctg gaaaatgaaa tcccctctga aaaaataaat gaaattgttg
      421 aaagggctta caattttgaa gttcctttag aaaaaataaa tgaaaattta tatattcttt
      481 atcttgacag aggaccaact gcttcattca aagattttgc agcaagattt atggctgaaa
      541 ttatgcaata tcttgcagat gaggaaaatt tgaatcttac agtacttgtt gcaacttcag
      601 gagatacagg tggagcaatt gccagtgcat tttttgataa agaaaatata aaagtggttg
      661 ttctatttcc tgaaagagaa ataacagaag ttcaaagaaa acaaatgact acattaaaca
      721 aaaacataat tccaattgct ttaaaaggga agtttgatga ctgtcagagt ttggttaaaa
      781 tggctttcaa cgacccagaa ttaaagaatt taaatttgac ttctgcaaat tcaataaata
      841 tttcacgatt attaccacaa agtgtttatt atttctatgc tttttcaaaa ttaaattttg
      901 aaaaaattgt cttttctgtt ccctctggaa attttggtaa cttaatggga ggtgttattg
      961 ctaaaaaaat gggtttacct gttgaaaaat ttatagttgc agtaaatgaa aatgatgaat
     1021 ttccaaaatt tctggaaaca ggagaatata aacccgtaat tccctcaaga aaatgtaatt
     1081 ctaatgcgat gaatgttgga cacccaagca atttagcgcg tttaattgat ttatatggtg
     1141 gctggattta tgatgaaaga agtatagatg gtaaagtaat aaaacatggt gtcttaaaag
     1201 aaaaacctga tatgaaaaag atgaaagaag attttgtttc attttcaata aaagatgaag
     1261 aggtaaaaga aacaataaaa aaatactatc agaaatataa aattatactt gacccacatg
     1321 gcgctgttgg aattgctgct tttgaaaaat caaatataga tgaacctgta atttcaattg
     1381 aaacagccca tccagggaaa tttccggaag taataaaaga agttcttgga attgaaccag
     1441 aaatgccgga gacactaaag aatctaattt caaaagaaga aaaatttatt gttcttgaaa
     1501 ataactataa tatattcaaa aaatttttaa aggacttaaa atgaaataca tagttatgat
     1561 tatggatgga gcagcagatt tgcctgttga agaactgaat aataagacac ctttacaggt
     1621 tgctaaaaaa ccaaatattg attatataac aaaattgggt tgttcaggga tgctgaaaac
     1681 aatacctgat aattttatta ctgattcagg agttgcaaat ttatcaatac ttggatataa
     1741 ccctaaaaat gtttatacag gaagaggcgt acttgaagca tatgcaaatg gaattgaact
     1801 taaagaaaat gaaattgcct tcagatgtaa tcttataact gtaaaagaag ggaaaattga
     1861 aagttattct gcaggatata tttcaacaga ggaaagtaaa gaattaattg attttttaaa
     1921 taaaaaactt ggtagtgaaa aggttaaatt ttatccagga cttggattta aaaatatact
     1981 cattttaaaa aatgattttt ctgaagatat taaatgttat cctccacacg attattacgg
     2041 atatgatata gaaaaaataa tgattgaagg aaaaacagaa aaagataaga aaacagcaga
     2101 attcttaaat aatctaataa ttgaatcaaa taaatatctt gaaaatcatc ctgttaattt
     2161 aaaaagagca aaagagggaa aagaaaaagc aaattatata tggccatggt cgccgggtaa
     2221 aaagccaaat cttgaaaaat tttatgataa atttaaaatt aaaggcgctg taatttcagc
     2281 agttgattta attaaaggaa ttgggaaaat gattggattt gaagttataa atgttcctgg
     2341 tgcaacagga ctatggaata caaactatga aggaaaagca gaatatgcta ttgaggcact
     2401 gaaaaaattt gattttgttt atgtacatgt tgaaggaatt gatgaggcag gtcatgaagg
     2461 aaatcttgaa ttgaagataa aatgtataga ggactttgat aaaagattgg taaaaataat
     2521 tcttgaaaat attgatttaa aatccaccag tattgctatt cttcctgacc attatactcc
     2581 tgttaaatta aaatcacatc aggtaggtta tgttccgttt gttatctatt ctcccctaaa
     2641 taaaccagac gaagttgaaa aatttgatga agaaagttgt aaaaatggta aaattggata
     2701 tattgaagga gaaaaattta taaatagttt tttaaagggg ttataagaat gtacataatt
     2761 attgttggtt gtggtaaaat tggttcttat cttgcaaaat tatttgatga aaagaatgat
     2821 gtggtcgtta tagataagga tgaaaagagt tttgaaaaac tgattgattt taatggaata
     2881 acaaaattag gagatgcact tgatatagat gttttaaaag aagcaggaat tgaaaaagca
     2941 gatgctatag cagtaattac aagtaatgat aatgccaata tagttatagg tcaggttgca
     3001 aaaaaaatgt ttaaagtacc aaaggttata ataagaattt ctgacccaaa taaagaaaaa
     3061 atatgcaaat tacttgatat agaaacaata aatacaacaa cccttattgc ttctcttata
     3121 aaagaagggc ttacaaaaaa aatatattca acccatctca tagaaaatga agattttgca
     3181 attgttgaat tgaaaaatga aaattttgtc gggaaaaaaa ttgaagaaat aaatgttaat
     3241 ggtgaacttc aaatttttgc tataataaga ggaaataaag gaattgttcc cgataaaaac
     3301 tttaaaattg aaaaagatga tataatcata ggtataactg aaaggaaaaa tttaaataaa
     3361 tttaaaagga ttttgaaata atgaatataa ttatagttgg tggtggaaat gtaggttatt
     3421 atttatctga aaaattgagc gataatcatt atgttgtttt aattgaaaag gaaacaaaaa
     3481 taagtgaaaa aattgcagaa aaattaaact gtcttgtaat aacgggtgat ggatgcgacc
     3541 ctgaagtttt aaaaaatgca ggaattaaga aagcagattg tctggctgct gtaacaggaa
     3601 gtgatgagga taatctggta atatgtcaga tagcaaaaga aatttttaat gttaaaaggg
     3661 tagttgcaag ggtaaataat ccaaaaaatg aaaaaacatt ctatcaactc ggtgttgatg
     3721 ttgcaataag tggaacttca ttgatagcaa aaattattga agaagaagta aattgggagg
     3781 actttataac attatttaca tttaaaaaag gaaaactttc aattttaaga attgacttac
     3841 ctgaaaaatc acctgtttta ggtaaaaaaa taaatgaaat taatttacca gatgactctg
     3901 ttatagttgc agttatgaga ggagatgatt tatttatacc taaaagtgat tttactttta
     3961 aagaaaatga tgaagttatc gcaataacaa aagtagaaaa tgaaaaccta cttttgaatt
     4021 ctttgatagg agaggtaaaa gaagtatgaa aaaaatttta aaatatatta atgtttttta
     4081 taaaggttct gccggaatat ttgtagaaat tttttatgtt ctttttatat tttttttgtc
     4141 tttggtttta aactttattc tttattcctc ttttaataaa cttattaaac ttccatgata
     4201 ccacaatttt caagagatga cttaaaaggg atttttcatt atactggaaa attaataatt
     4261 ttaattggct atttactgct attccccctt ttcattgcaa ttttatataa agaatggaat
     4321 ccagcacttg attttatatt tacatcttta ttttcccttt caattggata tttaattgtc
     4381 tcacaatttc ctgaaagttc tgatataaac tggagacaag gaatgacaat tgcttcattg
     4441 atatggctta tctatatgtt tcttggcgca atacccctct acttatcagg tcattataaa
     4501 tcttatatgg atagttgttt tgaagcaatg agtgctcttg caaccactgg acttgtactt
     4561 gttaatgacc ttgaccatat ggcacattct tataatttct ggcgacattt tatgtgtttt
     4621 ataggtggtc agggaataat tttacttgga ttattgattt tttttaaagg agcaagtgct
     4681 tttaaaattt acgtgggtga agcaagagag gaaaaaattc ttccaaatgt tgttcaaaca
     4741 tcaaaattca tctggattgt aagtacgtgt tatttgatag tttttacact aattttagga
     4801 tttgtaaata ttcttcatgg tttttctcct tcaagaggtt ttttccatgc tatatgtttg
     4861 tttatggctg gatgggatac cgctggtttt gcagtccaaa cacagaatat tgtttattat
     4921 cacagcggtc ttgttgattt gataactgct cttattgtta tacttggtgc aacaagtttt
     4981 gcccttcatt atgctgtctg gacaggaaaa ttcaaagaaa tatttgaaaa ttatgaattc
     5041 aaagtttttc tgataagtat tatttctctt acttttttat gttttgcggg atttttaaaa
     5101 agtgtcaaaa atattgattt tatttcgggt ttcagaattg ttttttatca gataatatca
     5161 ggacatacag gagttggata tacaaatatt gacccttatc atttcaaaac ttactggaat
     5221 gacttttcac tgataatttt aacaattgcg atggggcttg gtgggtgttc atgttcaaca
     5281 agtggaggga taaaaatttt gagaattgga cttattttta aagaaataaa aaaggaaatt
     5341 aaagggtaca gttatccttt ttccactgtt attattgaaa aatatcacca tataaaggat
     5401 ataagtttat ctgatgtaca gattaaaaca gtagcaataa tttcaataat gtatttagtc
     5461 acatatctta ttggaggaat aatcggaact tttttaggat atccatttat acattctctt
     5521 tttgaatcaa cttctgcatg tgcaaatgtt ggactttctg ttggaattac aaatccagat
     5581 atgccaggta ttttaaaaat aacatatata attgaaatgt ggattggaag gttggaattt
     5641 atttctattt ttgtctttat aaagtattta atctctttaa ggagtcccga atgaaaaaat
     5701 taatatttat tttacttttt tcaacactca tttattcatc tgaaaaagta aagataaaag
     5761 aactttttga aaatagcaaa aaatacaatg gaaaatctgt tattattgaa ggagaagcaa
     5821 tcggagaaat tatgggaaga gggaaagaaa aatgggtaaa tattaaagat gtatttgagg
     5881 acttcgctat tggagtagta gtgagtgaaa aggatgcaaa gattatagaa aatcttggta
     5941 gttataaagt taaaggagat attctaagaa tagagggaat atacaatgta aactgtttaa
     6001 aacatcaagg tgaaagggat atacatgcag ttagagttga aatcgtgaaa aagggcgaaa
     6061 gatataaaga agaaataatt ttaaaaaaag tgattttgtg ttttttactt cttataataa
     6121 cttcttttct tatgactttt gcacataaaa aagaaatttc tgcaaaagaa taaatttatt
     6181 aaactctcac aatacttcca taaagataat gtctctcccc tttttctatt tttactttga
     6241 aaattttacc ttccagtttc ttttcctctg tttcaactat aacaggttta ttattagttg
     6301 ttcttccaat gtatgaattt tttttcttat aactttcttt ttttataaaa acatcgtcaa
     6361 ttttaccttc tttcttttta ttttttaaaa gagaaatttt atcctgtaaa ttcaatatta
     6421 atctatgcct tttttctttt tcttcttttg ggacattatc ggggaattta tttgcttctg
     6481 ttaaaggcct tggtgaatat ttaaaaacat ataaatcatc aaattcaatc tcctcaataa
     6541 ctctatatgt ctgctcaaaa tctttttcat cttcataagg aaatcctaaa attatatcag
     6601 aagttattga aatatccggg caagcatttc ttgctctctg aacaatttca atatattttt
     6661 caagagagta ttttctattc attaatttga gtattttatt tgaacctgat tgaagtggaa
     6721 gatgtaaatg tttatataat tttggtaatt ctgaaaaaag atttattaaa tccgtagaaa
     6781 tgtcttttgg atgagaagtt aaaaaaccaa tttttaataa cccatcaata ttgtggactt
     6841 tggtaagtaa ttctacaaaa ttttctccat tatctttacc atattcatta acattctgtc
     6901 cgagtaaaat tacttctttt ataccctttt caacaagttt ttttatttct tctaatatga
     6961 taccaaccgg cttactgata agtttccctc ttgtataagg aacaacacaa taactacaaa
     7021 aattttcaca cccttttgta attgaaataa aagaagaaac agaactacct cttatttccg
     7081 gtaataaaat ctcattaaat aaatccatat cctcttgaaa tattcctatt tttttatcat
     7141 agaaattatt ttttaaaact tctacaaact tcttatacga atttggtcca catattatat
     7201 ctacaaaatc atatctttta aaaatttctt ccctgtataa tgaaggagta caaccaacaa
     7261 gacaaaattt tttattattt ttaaaatttt ttaaagaaga taaaaaagaa atggctctat
     7321 cttcagcatg ttttctaaca gaacaagtat ttacaattat ataatctgct tcttcaggaa
     7381 tagaaacttc aataaaattc tctcttttta atagtgctct taatctttcg gaatcataaa
     7441 aattcatctg acagccataa gtttttataa agaatttttt ggacatagta aagaaattat
     7501 acctttttta atgagaaaat tgaacctata attaaaccta aaagggtaaa agaattaata
     7561 ttaaatctta tagaaaaaga aagattgaaa ataatgaggt ctaattgttt aatttcccat
     7621 acaggagaaa ggtgatttga aagaaaattg taataaattg ttccctgagg aataaacata
     7681 agaattattt ctccaataca actacctatc attgcacata aaaataaaac agaaaaaaca
     7741 taaaaaattt tcatcaaaat aagtatatca taaaaaaaaa taataatcca tctttaattt
     7801 agtcacttgt aatttacata ccaaaagttt aaaaattatt tagattccta catttattga
     7861 cttctggcat tataaatatt aaaatataat aaatgtaaaa gcgcctgtag ctcaatagga
     7921 tagagcatct gccttctaag cagagggttg cgcgttcaag tcgcgccagg cgcaatttaa
     7981 aattttgggc cgttagctca attggtagag cggcggactc ttaatccgta ggccgcaggt
     8041 tcaagtcctg cacggcccaa aaagtattca aataaaacta aatcctgaaa caggaaaaaa
     8101 ttagttatcc aatataaaaa aggacttgaa agaagatatg ttttaagtaa ttgcgacaaa
     8161 taagaaaaat tataatgtca gttgtatcca aagtttacat tttctaaaaa gggaatatta
     8221 ctttgtagac ggaatcgcca agtcctgcat agcccaactt aatttttgat ttacaatttc
     8281 tccgtatttt atagctaacc cagatttcaa aggttgtgat atttctattg ctttcaaacc
     8341 tttatcagca agttgtaata tatatttttc tgttacatta cacagagcat atgttgaaga
     8401 ttttggaact attccaggga tattaggaac acaataatga acaatgcctt catatttata
     8461 aattggattt ttatgcgtag ttggatgact tgtttcaaaa acgcctccct catctattga
     8521 aacatctact ataacagtac cttcttccat aatcttcaaa tcttcttctt ttatcaatac
     8581 aggtgctttt ctacccggaa ttaaaacagc accaattaca acatcagcat ctttaatttc
     8641 tttttttatt ttttcttttt cagaatacag aattttccct ttaggaaatt ctctttttaa
     8701 tctttttatt ttttctttat ctatctcaaa aattacaacc tttgcaccaa ttgaataagc
     8761 aattttagca gcattatatc caacagttcc tcctccaaga attaccactt taccaggttt
     8821 aacaccctct gcaccagaca aaagaactcc tttacctcca tattcttttt caagatattt
     8881 cattccttcc tgaacactta atttacccgc tatttcactc attggtttta aaatagtaag
     8941 tattccttct tcctccacaa gttcataagc aatacaggat aatttttttt ctattagttt
     9001 ttctgtcatt tcaatattag atgaaaaatg aaaaaatgta aaaatcatct gaccttcaca
     9061 taataaatta tattcagaag gaaggatttc ttttacttta ataattaaat ctgaattttt
     9121 aaaaatataa gcatggtcat ttactatttc tgcacctttt tcctgataat cttcatcctt
     9181 tattcctaca ccaatccctg aatttttttc tattaaaacc ttatgacctt tttttaacta
     9241 gtttgctaac tgtttcaggt aaaatcccaa ctctgtattc ttcttcttta atttcttttg
     9301 gtattcctat tatcattttt gcatctcttt aataatttta attattgtat cagacatata
     9361 ttttaaatca acttttttaa aatcgtacag tggaagatgt gtaaaccgat gcaaaaaaac
     9421 ttcttctgca tttgggcatg tctcttttat tttttctgta tcatatccaa gttgtttcat
     9481 aattgaaaat ctgtataatg gagcaaaatg agggatttga gttattcctt tttctcccat
     9541 tttttcttta actttttgaa tgtctccatt taatacaccc gggtcaacct ggaaaagata
     9601 taaatgatgt gttgatttta tttttttatc atcaagagga gggattacaa ttcctttaac
     9661 atttttaaac ctttcattta aatattcagc cgcttttctt ctttttccaa taattttatt
     9721 tattcttttc atttgggaaa gtaaagcaac tgcctgaatt tctgtagcag aatgattccc
     9781 aggagcaaaa taatcacctc tccattttaa agcaacaaca tcataaagcc agaaaggaat
     9841 tggattggaa atattaaaac caacaaacct tgcttttgga aaatcctctc ctattttttc
     9901 atttgtaaca agaattccac cttctccaag agatgttatg tttttaacct catgaaaact
     9961 aaaagcacca aaatgtccaa ttgtaccaac catcttatca ttatattttg ctccaaaagc
    10021 atgagcagca tcttcaagaa ctaccgcatt aaattttctt gcaatatcca ttattgcatc
    10081 catatcacat ggataaccac ctatatgaac agggactata actttcgtgt tttttgtgat
    10141 ttttcttgct aaatcttcag gatccatatt taatgttttt gggtcaacat caaccagaac
    10201 aacttttgct ccaatagaaa gtggatagga aatggtagca ataaaagtaa ttgcgggcgc
    10261 tataacctca tctcccggtt ttaaaccagc aaatttataa gcaatttcaa aaccagcagt
    10321 agcatttgtc aaaaataccg catatttaca tttcaaaaac ttcttgactt cttcttctaa
    10381 agttctaact ttatctccaa gtgtcaattt actggaaagt tctgaatttt caatcaattt
    10441 ttttaattct ttttcaactt ttttcaattg aattttatat tttttatttt ttggagggat
    10501 aagaaatttt aaaatctcca taatatcctg taattttatt ttctctccaa cagcactcca
    10561 tgggattctt gcaggacctg tatcatatct aaaatccgaa atttttttct ttttcatatt
    10621 tatctccttt ttttaccttt ttgtaaataa tattttaaaa aaaattaaat gtcaagtcct
    10681 aaaaaattac ttataacaag acatgcgcct ccaattgccc ctatttcttc gccaaaatct
    10741 gaaatttcaa aactcacagg atactcataa accatatttt cttttatttt ttcaaaaata
    10801 tttttaaatt ccttctcaaa aacaagaaaa tttttatcaa atatcacaat ttcaggattt
    10861 aaaatattta ttaaattaga gacagaaaaa accagaatat taaaaacatc atccataaat
    10921 ttttttatct cactatctcc cttttcatac ctttcaataa tttcattcat tttaagttct
    10981 gtccctttaa catcttttat ttttttcaaa attgctggaa cacttattat tgattcaaga
    11041 catcctcttt taccacaatt acagatttca tctttataag gtattttaac atgtccaaat
    11101 tcaccagcaa ctcctccttg tccatgaatt attttattat catttataat tccacaggca
    11161 atcccttttc catattcaat aaaaattata tttttacttt ttctaccctt acccagaaat
    11221 ttttcactca aaacttttga ttgggaacta ccaattataa gtgtattaat tgataatttc
    11281 tgttttaaat aatctcttat atgtacattt tcccatcccg gtaaaattga agaaaaaaca
    11341 gaaactccct tttgcatatc aactattcca gggtctacaa aacctatccc acatatttta
    11401 tttttttcta ttttgctttc ttcaataaga tttttcaata aatttacaat ttttattaaa
    11461 atttcttctt ttttatctgt ttcatccaag tttactgttt tttgaccaat aaattctcct
    11521 ttaaaattta aaataactga aattattttt tctggggtta actcacatcc aataaaatac
    11581 ttactgttag gtattatttt aagcaattcg tgaggtctcc ctttactttt tctttttctc
    11641 ttaccagatt cttcaattat cccttctttt attaaatctc ttgtaatatg tgtaattgtt
    11701 gcaaatctta aatttagtaa atcatgcaaa ttctttcttg aaatattttc attttgataa
    11761 atcaatttaa gaatgttttt tctgtaaact gtatatttag aaaccatttt tttccttttt
    11821 ataaatttaa tacttttcta ttattttaca ttacattcaa agattttcaa aaatataaaa
    11881 attaaaatgt atgatataat ttttttcgtt atgggaaaag aagtaattat tttagaatgt
    11941 aaggattact cagttgactt aatttatgaa aagttaaaaa agggaattga attacttaaa
    12001 attaataaaa ttcctgaaaa aattcttata aaaccaaata tgctctctgc aagacaacca
    12061 gaagaaggag ttacaacaca tccagcaatt atatccgctt tgattgagat ttttaaaaat
    12121 aaaaaaatta ttataggaga cagtccagca ggtatatcaa aaccaattga agaatactgg
    12181 gaaaaatgtg gttataaaga aataggggat aaatataaaa ttgaactaaa aaagtttgaa
    12241 aattctttcc ctttggatat ttgcgttaat aacaaagttt ataaaattcc tgtgacttcc
    12301 ctgttaaacg atttctctat attaaatgct ccaaaattca aaacacataa cttaacaacc
    12361 atcactgtcg gtgttaaaaa tttatatggt ttgatacccg gaataataaa aagttatctt
    12421 cacagtaaat ttataactcc atatgaattt tctttttttc ttatagagtt ttataaaaaa
    12481 atagaggaaa aaattttttt aacagtagtt gatggaataa caggaatgga aggagacgga
    12541 ccatctgccg gtaatttaag aaatattgga tatttaataa ttggagaaaa gccgatatat
    12601 gttgattatg catgctgtaa actgattgga ataaaaccag aaaaggttga ttttatagaa
    12661 atttatatgg ataaatatgg aattgaaaaa cctgaaataa tcggtgattt taaggaaata
    12721 aaaaattttc aacttccctc cacaaaaaaa tattttttta tcagaaataa acttctatca
    12781 aaatttattt taccaattgc aaaatttttt aaaatagccc ctgtaataga taagaaaaaa
    12841 tgtaaaaaat gttttacctg ctataatatt tgccctgtaa aagcaatttc taacgacttg
    12901 aaatttaata gaaaaaaatg tatattatgt ctatgctgtt ttgaagtttg tccatataaa
    12961 gcaattgaga taaaaaagag ttttattgca agaattttta cttaaaaaag gagtaaaaat
    13021 ggattatgaa aaaattttat ccgaaataat acagaaaaat gataaaaaaa tattattgct
    13081 tgttcttgat ggagttggtg gacttcccca tcctgaaact ggtaagacag aacttgaaac
    13141 agcaaatatt ccaaatcttg attattttgc agaaactggt tcctgtggac ttattatacc
    13201 tgttttacct ggtataactc ccggaagcgg gccgggacac attgcacttt ttggatatga
    13261 ccctatagaa gttcaaattg gaagagggat tcttgaatgt ctcggaattg gacttgaagt
    13321 aaaaaaagga gaaattgcca taagaggaaa ctttgcaaca attgacaaaa atagaatagt
    13381 tatagataga agagcaggaa gaatacctac tgaagaaaat gaaaaaattg taaaattgat
    13441 ttctgaaaat ataaaagaaa taaaaggagt aaaattcaaa attcagacag ttaaagaaca
    13501 cagatgtgct ttaatacttt caggagaaaa tttatcctgt gatgtaactg aaaatgaccc
    13561 cgggaaagaa ggactaccac ttaaaaaatt tgaaccgctg ggtgaaaaag gaaagtttat
    13621 agcagaaatt ttgaatgaat ttgaagaaaa agtaatttca atccttggaa gtttaaactc
    13681 aaaagcaaaa tgtttacttt taagaggatt ttcaacttat cctgatatac aaacattcaa
    13741 agaaagatat aaacttaacg ctgtctgtat agctacatat ccgatgtaca ggggaatttc
    13801 aaaacttgtt ggaatggatg ttattgattg tggggaaacc attgattctg aaatagaaac
    13861 actgaaaaaa gtttataatg attatgattt ctttttcctt catattaaaa aaacagacag
    13921 tttcggagaa gatggtaatt ttgaaggaaa ggttaagata cttgaagaaa ttgataaaaa
    13981 aattgaaccc attaaaaata tgaattttga agcaattgga attacaggag accactcaac
    14041 tccatctctg ttgaaaagtc attcctggca tccagttcca tttgtactta tttctccaaa
    14101 tactctaccg gatgacgtta aaagatttac tgaaagagat tgtgcaaaag gaattcttgg
    14161 aaatatttat tcaaaaaatg ttatgtatct cttacttgca tgttctttga aacttgctaa
    14221 attcggagcg taaaatgttc agaggaggta ttaagttaat agattttaaa agtttaagca
    14281 aaaatgagga aattgaagaa tttaaatatc ctgaaagggt tataatacca ctctcacagc
    14341 atacaggaag tcctgcaaaa ccagttgtaa aaaaaggaga ttatgtcctt gaaggacaga
    14401 taataggtga aattacaggt tttatcagtt catatattca ttcctctatt tctgggaagg
    14461 ttgttgatat attaccatgg gatttaccaa caaacagaaa atcattgagt atagttattg
    14521 aaagtgatgg agaaagaaaa tctattgaaa gacaggtaag ggactggctg aaattggaaa
    14581 gtaatgaaat tataaatata g
//
LOCUS       DAOSAT010000044        14574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2803_length_14574_cov_7.864316, whole genome shotgun sequence.
ACCESSION   DAOSAT010000044 DAOSAT010000000
VERSION     DAOSAT010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14574
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2803_length_14574_cov_7.864316"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2888)
                     /locus_tag="PLF90_05000"
     CDS             complement(<1..2888)
                     /locus_tag="PLF90_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOK56668.1"
                     /translation="MYGFGKDREPLDLTFIVNEPGKTLKDRFAQLIKDCKFFDCLVAY
                     FKVTGFYQIYKALENTEKIRILIGIGTDKESFELIESAEGNASHYETKKCISEIIQTE
                     FESTDEDEKETEEGVLKFIEWINQGKIEIRAYPNKRLHAKVYIMTFKEEDRDIGRVIT
                     GSSNFTYSGLVDNLEFNVELKNRADYEFAKKKFEELWNIAVDVKENYIRAIKERTWLN
                     PDIAPYELYLKFLYEYFKDELSSTDKVFTKYLPPDFKKLEYQEQAVLNAKKILEEYGG
                     VFISDVVGLGKTYVATMLSGQLDGRTLVIAPPALLNRNNPGSWPNVFSDFHIPAEFFS
                     IGKLDEAKEFVERYENTEQPVKNIIIDEAHRFRNETTISYEDISEICRGKRVILVSAT
                     PYNNSPRDILAQIKLFQNPRKSTIPGILDIEKFIVGLDERLKKINRQKDYDKCLEITK
                     QNAKEIRDKILKYIMVRRTRKEIEKYFSEDLKKNNIKFPEVEDPKLFYYQLNDEEDEI
                     FMETVKKITQEFKYTRYTPLLYLTKKITPLEEQAQRNMGGFMKVLLVKRLESSFYAFR
                     KSIDRFIRSYEMFIKEYEKGNVYVSKKYINKIFELLEQGDDEAIQRLIDEGKAERYAS
                     SDFRPDFKNYLEHDLKILKKIKSMWDEVKRDPKLETLIENLKKHLVLRNSKKIIIFTE
                     SKETAEYLTENINKEFGEIALLFHGNSPELVRDKVIENFDARARNKRDDYRILVSTEV
                     LSEGVNLHRSNIVINYDIPWNPTKLMQRVGRVNRIDTQFDKIYTFNFFPTRQADSEIE
                     LTNIARSKIEAFLTLLGGDSAILTEGEPVSSHELFDKLLSKKTITEDEEEESELKYLR
                     IIENIRDKNPEFFERIKRLPKKARSAKIFTEDLKDFATPNSLLTFFRKGKLMKFFLSN
                     KEKRVELDFLTAAKILESTQNEKREELPLEDYYEFLDKNKTAFFNATL"
     gene            complement(2919..4151)
                     /locus_tag="PLF90_05005"
     CDS             complement(2919..4151)
                     /locus_tag="PLF90_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOK56669.1"
                     /translation="MENLISFEKAIEIVLKETKICGVEKVDILKSLGRFIAEDIFSPF
                     DMPPFKRSAMDGYAIKFDDLSNTKIFEIKDEIKAGEEKIVEYEKGKCVLIMTGAKIPE
                     GFDTVIKFEDTEEFVEEGKRKIKILKEIKKGENIAEKGEDFKKGDLLIKKGTFIDSGY
                     MAIIAYCGIKKVLVYKKPEVGIIATGDEVKKIGEKLREGEIYDCNSYSIYGLVLKYGG
                     IPEILGIAKDNKRSLKRYIEKGLEKDILILSGGVSEGKYDFVVDVFKEIGIEMKFWKV
                     AVKPGKPTFFGKKGKTLIFGLPGYPVSTYINFENLVRPAILKMQGKKNFDRFKVVGIL
                     ASDVENKGDRDEFLRIIVKVENDENIVIPFKKQKSGVFTSIVNTNGLLYLKKGQKIKK
                     GEKVIVSVMRSDLVSIFT"
     gene            complement(4129..4722)
                     /locus_tag="PLF90_05010"
     CDS             complement(4129..4722)
                     /locus_tag="PLF90_05010"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOK56670.1"
                     /translation="MNSIILAKGEKSKRTGLDKALIEINSKKLIEIIIERIKPVFDKI
                     YIVSLNPEKFENYKDEKIDVIKDGFGCGPLGGIYTGLSISSSLYNFVFACDMPFINVD
                     FVRYMMGIKKNYDVLVPVYKNRYEVLFAIYSKNVLKVMEEMIKRGEYRVREILNRAKV
                     EYISQDIIEKFGWQESFFNLNTKNDILILKHGKFNKF"
     gene            4752..4892
                     /locus_tag="PLF90_05015"
     CDS             4752..4892
                     /locus_tag="PLF90_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56671.1"
                     /translation="MKNLIAIFSGLIIGLMAGLIGVGGGAATVRMLTDEVGPCKCGRN
                     RF"
     gene            complement(4846..7545)
                     /gene="fdhF"
                     /locus_tag="PLF90_05020"
     CDS             complement(4846..7545)
                     /gene="fdhF"
                     /locus_tag="PLF90_05020"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOK56672.1"
                     /translation="MENKKIKIEIDGKEIFTEIGKTILQTAKENGIEIPNLCNHPELI
                     PNGACRLCLVEVVGEKRPVASCCYPVYDGMKVYTNTEKLINYRRLTLQLIISDHPLDC
                     MTCEKSGDCLLEKYAYQYGIKKIEYSGEKAERKEKNGFPFIVRDYEKCILCGRCVKVC
                     EEIVGANAIDYKNRGFITEIISGFDEILKDTECLFCGNCIEVCPVGSLREISAEGKGR
                     NWEYEKIKAICPYCGVGCGIVAYVKNNEIVKVKGDEDSPVNKGFLCIKGKFGFEYVKD
                     EERLKKPLIKEDGKFKEVSWEEAIDFVYEKLKYFRDNFGPFSIAGLSSAKCTNEENYI
                     FQKFFRCVLKSNNVDHCARLCHSSTVVGLSQTIGSAAMSNSLDEIENYSDCIIITGTN
                     ITETQPVTSYRIRKAKEKGAKIIVIDPREIEISKIADIYLQPRYGTDVIVFNGLAKII
                     VDEKLYNEEFIKERVENFEEYKKFIEKFDLSLVEKIANVKIEDLRKVAYLYSKSKSSV
                     ILWSMGITQHITGTENVFTLSNLALLTGQIGKKGAGLSPLRGQNNVQGACDMGCLPEF
                     YPGYSRVDDKNTIEKFENLWNEKGLPDKIGFTLVEMFENVLKGNIKCLYIMGEMPLIS
                     DPDILHIKKALEKIDFLIVQDIFSTEVCEFADVVFPALSSFEKEGTFTNTERRVQKIS
                     KCIENNNGKPDWWIINEIAKRFGYNWNYNDVWDILREINKTVEVYKGITPKRVKNGDR
                     IQWPCLSEEDKGTETLHIGKFKRGKGILKIVEYKEPYELPDEDYPFILTTGRILTEYH
                     SRTMTGKVKGIKEITGDEFFVMNCEDAEKLGIKEGDFAEIKSRRGSIKLKAKLSRKIQ
                     KGVIFIPFHFYANILTHTELDPYSKIPEFKICSCHICKVPPRQ"
     gene            complement(7548..9350)
                     /locus_tag="PLF90_05025"
     CDS             complement(7548..9350)
                     /locus_tag="PLF90_05025"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOK56673.1"
                     /translation="MKFKNFSDFKNYCEKLKKEKDERVKIKVCITGCRAAGGLDIYEN
                     FKEKIKESKKLKLIPTGCQKFCSGGPVVSVSFDGKSFLYEKVSLKDVESIIKKAEKKE
                     VVEELVIKDEKFLKNQTFKVLKLCGEIDPLDIDEYIKNGGFSSFLKVLNEYTPEKVIE
                     ELKKSKLRGRGGAGFPTYLKWEIVKNKQDDEKYLICNGDEGDPGAFMDRTLLEGVPFQ
                     VIEGMLIGAYTTGCKKGYFYVRAEYPIAIEHIEKALRICYEYGILGKNILGTDFSFDI
                     ELKKGAGAFVCGEETALIASIEGKRGMPRPRPPFPAEKGLFGKPTCINNVETFANIPI
                     IIEERGENFSKIGTEKSGGTKIFSLAGKIKNTGLVEIPLGTPLWKIIYEIGGGVPEGR
                     KIKGVQTGGPSGGCIPVELFNTPVDYETLANLGSIMGSGGMIVIDDKVCIVELARYFL
                     DFVQKESCGKCVPCRIGTKRMLEIIEKITEGKANIEDIEKLEELGNYIKETSLCGLGQ
                     TAPNPVLTTIKYFKDEYISHIKEKKCSSGVCVNLIKYYINPEKCTGCTLCAKNCPVNA
                     IEGEKKKPHKINVEKCIKCGICFNICKFGAVEKD"
     gene            complement(9347..9793)
                     /locus_tag="PLF90_05030"
     CDS             complement(9347..9793)
                     /locus_tag="PLF90_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOK56674.1"
                     /translation="MNEIKEIIEEIENKNFSNLEILQYLQEKYNYVPIETLKKICEEI
                     EIPRSKIFGILTFYSQFSTEERGKYLIRVCDGTACHVKGGSKIIEMLKREFKLENGKT
                     TEDKKFTLQVVACLGSCFLAPVCMINSSYYGNMDENKFKKILEKLK"
     gene            complement(9786..10703)
                     /locus_tag="PLF90_05035"
     CDS             complement(9786..10703)
                     /locus_tag="PLF90_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOK56675.1"
                     /translation="MKGIVYSVNIGEKKGEGKRPVEKIELIENYGVKGDCHAEIGINR
                     QVSLLSWERMNEEFFCLKKETGLSPGVFAENITTGKIDLRKLKIGDKLKINDVVIEIS
                     EIGKKCHNWCEIYKKVGKCLMPKEGVFGKVIKGGEIKKGDIIEVIPRIYIGILTISDG
                     CFKGEREDKSGKYLKEICKELGWDVLKYDIVADEKEMIKNKLNEFSEVCDLILTTGGT
                     GITKRDITPEATKEVIEKEIPGVSEILRIKTYEKSKFSVISRGVCGIKGETVIINLPG
                     SLNGVKECFEVLKDFLVHLIEMVRDFPHE"
     gene            complement(10700..11101)
                     /gene="moaC"
                     /locus_tag="PLF90_05040"
     CDS             complement(10700..11101)
                     /gene="moaC"
                     /locus_tag="PLF90_05040"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005368059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOK56676.1"
                     /translation="MIDVGDKKITKRKAVAEGFIIVAPEVIDKIKNNKVPKGNVFDFA
                     ESAGLFAIKNTPYTIPHCHPVKITKAKINFEIENDKIRVICEVEGIDRTGFEMEALYG
                     VCVSLLTIYDMCKGFKKEMKITDIKLLSKQK"
     gene            complement(11098..11841)
                     /gene="fdhD"
                     /locus_tag="PLF90_05045"
     CDS             complement(11098..11841)
                     /gene="fdhD"
                     /locus_tag="PLF90_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOK56677.1"
                     /translation="MIKKVEIVKFTEGLGENKLEDEVVEEKYVRIFINGKEVVVLSCL
                     FENIDYLIYGFLFTQGIVKDLKEIENIDISGCICNVRLKKNDFLGPFWFISSECGSRH
                     ITLNIMDKVNFDNFFKSDAIFYLLKVFNRISQLYKKTGCVHVCGIAKDREIIIFKEDI
                     SRHNAFDKVIGEAILKNISLNDKILLTSGRITSEIIFKLVKMKIPVILSKGAPTDYAI
                     RIGEEFGITIIGFARGKRFNIYTHKERIK"
     gene            complement(11841..12800)
                     /gene="moaA"
                     /locus_tag="PLF90_05050"
     CDS             complement(11841..12800)
                     /gene="moaA"
                     /locus_tag="PLF90_05050"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOK56678.1"
                     /translation="MKDKFGRKVDTLRISITDKCNFRCLYCSKEFYKLNKKNILSYEE
                     IVDIVKFFTNFGIKKIKITGGEPLIKKNIYYLINKISKIDGIKEVSITTNGYFLCENV
                     ERLKKVGVKRVNISLDTLKSEKFEGITSVKGLEKIICGIKESVKNFDVVKLNIVLMKE
                     INLDEIYDFLKFAEENKVILKFIELMPVYKNYTFWKENFVPYFFVEEILKKYIQLSPI
                     CENGRIKKYENKDKTIYIEFVTPVSEPFCKNCDRLRIDCKGNLFSCLYGEPIFNFKNF
                     NNKENEILGIIFSLIFNRKFSYNPFLEIQRSYYKLPYMNIVGG"
     gene            complement(12800..13219)
                     /locus_tag="PLF90_05055"
     CDS             complement(12800..13219)
                     /locus_tag="PLF90_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOK56679.1"
                     /translation="MKSFTQYLWFCTENRIEFINITEKVEEAVKKSGVKEGICLVNAM
                     HITASVFINDDEKGLHSDFVEFLEKLAPHQPISKYKHNLTGETNADAHLKRTIMGREV
                     VVAITNGRLDLGPWEQIFYGEFDGQRKKRVLIKIIGE"
     gene            complement(13319..13612)
                     /locus_tag="PLF90_05060"
     CDS             complement(13319..13612)
                     /locus_tag="PLF90_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56680.1"
                     /translation="MKWWTWEKGDKMANQKISFKTKKELEKTILHEKAKGTTDLEIGQ
                     KYRVTFRYIERFITRSQGLNISSFKVSKKIKSLHPRDFKEEQTTVWSLKQRGN"
     gene            complement(13769..14392)
                     /locus_tag="PLF90_05065"
     CDS             complement(13769..14392)
                     /locus_tag="PLF90_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56681.1"
                     /translation="MPIDFNEWAAEYERNYRRKNISIQYYIDGNGEIAHEYSPYFESI
                     SQVLQQRGFLSKKEFISICEWKTKRQKNRYQENSEEDIMEITNVVITGDLNIEGQIYK
                     LTQLEGVGVPVASAILTVVFPNNYCVLDYRVWRALLWVMSETDGFGDYTEFSEIMGNF
                     LNYASFGSYMYYLEKIRTLAEEHNMTPRKIEMALWMYDKKGGILINE"
BASE COUNT         4454 a         2852 c         1498 g         5770 t
ORIGIN      
        1 tccaaagtcg cattgaaaaa agcagtcttg ttcttgtcaa gaaattcata gtaatcttca
       61 agtggcagtt cttctctttt ttcattttgt gtgctttcaa gaattttagc agcagttaga
      121 aaatcaagtt caactctttt ttctttattt gataaaaaga atttcataag tttccctttt
      181 ctaaaaaagg taagtagtga atttggagta gcaaaatctt ttaaatcttc agtaaatatt
      241 tttgcagacc gcgctttctt tggaagccgt tttattcttt caaaaaactc tggattttta
      301 tcacgaatat tctcaataat tcttaaatac ttaagttcac tttcttcttc ctcatcttct
      361 gtaatagttt tcttagagag gagtttatca aagagttcat gagaagatac tggttctcct
      421 tctgttaaaa ttgctgaatc accaccaagt aatgttaaaa atgcttctat ttttgagcga
      481 gcaatatttg taagttcaat ttcagaatct gcttgtcttg tcgggaaaaa gttgaatgtg
      541 taaattttat caaattgtgt atctatacga tttactcttc caaccctctg cattaacttt
      601 gttgggttcc aaggaatgtc ataattgata acgatatttg agcgatgaag attaacacct
      661 tctgaaagaa cctctgttga gacaagtatc ctataatcat ctcttttatt tcttgctctt
      721 gcgtcaaaat tttctattac tttgtctcta actaattccg gagagttacc atgaaaaagc
      781 aatgctattt ctccaaactc tttatttata ttttcggtta aatattccgc tgtttctttt
      841 gattctgtaa agatgattat tttcttacta tttctcaaaa ctaggtgttt ttttaaattt
      901 tcaatgagtg tttcgagttt tgggtctctt ttaacttcat cccacattga ttttattttt
      961 ttcaaaattt taaggtcatg ctccaaataa tttttaaaat caggtctgaa atctgaactc
     1021 gcgtatcttt ctgctttacc ttcatctatt aatctttgaa ttgcttcatc atctccctgt
     1081 tctaaaagtt caaaaatctt atttatatat tttttactaa catatacatt ccctttttca
     1141 tattctttaa tgaacatttc atatgacctg ataaatctat caatactttt tctgaatgca
     1201 tagaaactgc tttcaagacg tttaacaagt aaaaccttca taaatccacc catatttcgc
     1261 tgtgcctgtt cttccagagg tgtaatcttt tttgtgagat ataataatgg agtataacga
     1321 gtatatttaa attcctgcgt tatctttttc acagtttcca taaatatttc atcttcttca
     1381 tcattgagct ggtaataaaa tagctttggg tcttccacct cgggaaactt gatattattt
     1441 ttctttaaat cttctgaaaa atacttttca atctcttttc ttgtgcgtct caccattata
     1501 tatttcaaaa tcttatcgcg aatttctttg gcattctgtt tagttatttc aagacattta
     1561 tcataatcct tttgtcggtt tatttttttt aatctctcat caagtccgac aataaatttt
     1621 tcaatatcaa gaatccccgg gatagtgctt tttctcggat tctggaaaag ttttatctgg
     1681 gcaaggatat ctcttggtga attgttgtaa ggagtagcag agaccaaaat taccctttta
     1741 ccacggcaga tttcagaaat atcttcataa ctaattgtag tttcatttct aaaacggtgt
     1801 gcttcatcta taattatgtt ttttacaggt tgctcagtat tttcatatct ttctacaaac
     1861 tcttttgcct catcaagttt gccgatggag aagaactcag caggtatatg gaaatcagaa
     1921 aagacattgg gccaggagcc tggattattt ctattaagaa gcgctggtgg agctatgacg
     1981 agcgttcttc catcaagttg tcctgagagc atagttgcaa cataggtttt tccaagtcct
     2041 actacatctg agataaagac cccgccatac tcctctaata ttttctttgc attcagaaca
     2101 gcctgttcct ggtattccaa ttttttgaag tctggaggca agtattttgt aaaaacttta
     2161 tcagtactgg aaagttcatc tttgaagtat tcatacaaaa atttaagata aagttcataa
     2221 ggcgctatat cagggtttaa ccatgttctt tcttttattg ctctaatata attttctttc
     2281 acatctactg caatattcca taattcttca aatttctttt ttgcaaattc ataatcagca
     2341 cgattcttta actcaacatt aaattcaaga ttatcaacga gaccagagta tgtaaagttg
     2401 cttgagcctg ttattactct tcctatatct ctatcttctt ccttaaaggt cataatatat
     2461 acttttgcgt gaagtcgctt attagggtaa gcccttattt ctatttttcc ttgatttatc
     2521 cattcaataa atttcaagac accttcttct gtttcttttt catcttcatc tgtgctttca
     2581 aattctgtct gtataatttc gcttatgcac ttttttgttt cataatgtga agcatttcct
     2641 tccgcagatt caatcaattc aaatgattct ttatctgttc caattccgat caaaattctt
     2701 attttttctg tgttttcaag agctttgtat atctggtaaa atcctgtaac cttgaagtat
     2761 gcaaccagac aatcaaaaaa tttacaatcc tttattaatt gtgcaaatct atctttaagt
     2821 gttttacctg gttcgtttac tataaatgtt aaatctaatg gttctctatc ttttccaaat
     2881 ccgtacattt ctcatttgat tatactatat ttttatcttt aagtaaaaat ggatacgagg
     2941 tcggacctca ttactgaaac aatgactttt tctccttttt ttattttctg tccctttttt
     3001 aaataaagca atccatttgt attaactatt gaagtaaaga cacctgattt ttgcttttta
     3061 aacggaataa ctatattttc atcattttca actttcacaa ttatccttaa aaattcatct
     3121 ctatcacctt tattttcaac atcacttgct aaaattccta caactttaaa tctatcaaag
     3181 ttcttttttc cttgcatttt taaaattgct ggtcttacaa gattttcaaa gtttatatat
     3241 gttgaaactg gatatcctgg aagaccaaaa attaatgttt ttcctttttt accaaaaaat
     3301 gttggtttac caggttttac tgcaactttc cagaatttca tttcaatacc aatttcctta
     3361 aaaacatcaa caacaaaatc atacttaccc tcgcttactc cacctgataa aatcaaaata
     3421 tccttttcta accccttttc aatatatctt ttcaatgacc ttttattatc ctttgctatc
     3481 ccaagaattt caggaattcc accatatttt aaaacaagtc cataaataga ataagaatta
     3541 cagtcatata tttctccctc tctcaatttt tcccctattt tctttacctc atcaccagtt
     3601 gcaattattc caacttctgg ctttttataa accaaaactt tctttattcc acaatatgca
     3661 ataatagcca tatatccact atctataaaa gtccccttct ttatcaataa atctcccttt
     3721 ttaaaatctt ctcctttctc agcaatattt tcaccctttt taatttcttt taaaattttt
     3781 atcttccttt tcccttcctc aacaaactct tctgtatcct caaatttaat aacagtatca
     3841 aaaccttctg gaattttagc acctgtcatt attaaaacac attttccctt ctcatattca
     3901 acaattttct cttctcctgc tttaatctca tccttaatct caaatatttt tgtattactc
     3961 aaatcatcaa attttattgc ataaccatcc attgcacttc ttttaaatgg tggcatatca
     4021 aaaggagaaa aaatatcttc tgcaataaat cttccaagtg attttaaaat atcaactttt
     4081 tcaacaccac aaatttttgt ttcttttaaa actatttcaa ttgctttttc aaaacttatt
     4141 aaattttcca tgttttaaaa taagaatatc attttttgta tttaaattaa aaaaactttc
     4201 ctgccaacca aacttttcaa ttatatcctg gcttatgtac tcaacctttg ccctatttaa
     4261 aatttccctt actctgtatt cccctctttt tatcatctct tccattactt ttaaaacatt
     4321 cttactataa atcgcaaaaa gaacttcata tctattttta taaacaggaa ctaaaacatc
     4381 ataatttttc tttattccca tcatatatct aacaaaatca acatttataa aaggcatatc
     4441 acaggcaaaa acaaaattat ataaagaact tgagattgaa agaccagtat aaatccctcc
     4501 aagtggtcca catccaaacc catcttttat tacatcaatc ttttcatcct tataattttc
     4561 aaatttttca ggatttaaag atacaatata aattttatca aaaacaggtt ttatcctttc
     4621 aataataatc tcaatcaatt ttttactatt gatttcaatt aatgccttat ctaaaccagt
     4681 ccttttactt ttttctcctt ttgcaagaat tattgagttc atataaaata aattaccata
     4741 aaggcatcat aatgaaaaat ctaatcgcta ttttttctgg tttaatcata ggactaatgg
     4801 caggacttat tggtgttggt ggaggagcag caacagtaag aatgcttact gacgaggtgg
     4861 gaccttgcaa atgtggcagg aacagatttt gaattcaggg atttttgagt atgggtcaag
     4921 ttctgtatgg gttaatatat ttgcataaaa atggaatgga ataaaaataa cacctttttg
     4981 gatttttcta ctcaattttg ctttcaattt gatagaacct cttcttgatt taatttctgc
     5041 aaaatctcct tctttaattc caagtttttc tgcatcctca caattcataa caaaaaattc
     5101 atctccagtt atctccttta ttcccttaac ttttccagtc attgtccttg aatgatattc
     5161 tgttaaaatc cttcctgttg ttaaaataaa tggataatct tcatcaggca attcatatgg
     5221 ttctttatat tcaacaatct ttaaaatgcc ttttcctctt ttaaatttac ctatatgcaa
     5281 tgtttctgtt cctttatctt cctctgatag acatggccac tgaatcctat ccccattttt
     5341 tacccttttg ggtgttatcc ctttataaac ttcaacagtt ttatttattt cccttaaaat
     5401 atcccagaca tcgttataat tccagttata accaaatctc tttgcaatct catttattat
     5461 ccaccagtca ggttttccat tattgttttc aatgcatttt gaaattttct gaaccctcct
     5521 ttctgtattt gtaaatgtcc cttctttctc aaatgaagaa agtgctggaa aaacaacatc
     5581 tgcaaattca caaacttcag tagaaaagat atcttggaca attaaaaaat caatcttttc
     5641 aagtgctttc tttatatgta aaatatcagg gtcagaaatt aaaggcattt ctcccattat
     5701 atacaaacat ttaatatttc cttttaaaac attttcaaac atttcaacaa gagtaaaacc
     5761 aatcttatca ggaagtcctt tctcattcca tagattttca aatttctcaa ttgtattttt
     5821 atcgtcaacc ctactatatc cgggataaaa ctcaggcaaa cagcccatat cacaggcacc
     5881 ttgaacatta ttctgtcccc ttaatggaga aagtcctgca cctttttttc caatctgacc
     5941 tgtaagaaga gcaaggtttg aaagagtaaa tacattttct gttcctgtta tatgctgtgt
     6001 tatccccata gaccataaaa ttacagaact ttttgatttt gaatataaat aagcaacttt
     6061 ccttaaatcc tctattttta catttgcaat tttctcaaca agtgataaat caaatttctc
     6121 aataaatttt ttatattctt caaaattctc aactctttct tttataaatt cctcattgta
     6181 aagtttctca tcaacaatta tttttgcaag tccattaaaa acaattacat ctgttccata
     6241 tctcggctgt aaatatatat cagcaatttt tgaaatctct atttctcttg ggtcaataac
     6301 aataattttt gctcctttct cttttgcttt tcttattcta taagatgtta ctggttgtgt
     6361 ttctgttata tttgttcctg ttataattat gcaatcagaa taattttcaa tctcatcaag
     6421 tgaattactc attgcagcag aacctattgt ttgagaaagt cctacaactg ttgaggaatg
     6481 acagagccgt gcacaatggt caacattatt tgattttaaa acacacctaa aaaatttctg
     6541 aaatatataa ttttcttcat ttgtgcattt tgcagatgaa agtccagcaa ttgaaaaagg
     6601 tccaaaatta tctctaaaat attttaattt ttcataaaca aaatcaattg cttcttccca
     6661 acttacttcc ttaaatttac catcttcttt tataagtggc ttttttaatc tttcctcatc
     6721 ctttacatat tcaaatccaa actttccttt aatgcataaa aatcctttat ttacaggact
     6781 atcttcatct ccttttactt tcacaatctc attatttttt acataagcaa caattccgca
     6841 gccaactcca caatatggac atatagcttt tatcttttca tattcccagt tccttccttt
     6901 tccttcagca cttatttccc ttaaagaacc aacaggacat acttcaatac aattaccaca
     6961 gaatagacat tctgtatctt tcaatatttc atcaaatcca cttattattt cagttataaa
     7021 ccctctattt ttataatcaa ttgcatttgc accaactatt tcttcacata ccttaacaca
     7081 tctaccacat aatatacatt tctcataatc cctaacaata aaaggaaatc catttttttc
     7141 ttttctttct gccttttctc cagaatattc aatctttttt attccatact ggtaagcata
     7201 tttttcaagc aaacaatctc cacttttttc acatgtcata caatcaagag gatggtctga
     7261 aattataagt tgtaaagtta atcttctgta gtttatcagt ttttctgtat ttgtataaac
     7321 tttcattcca tcataaacag gatagcaaca tgaagcaact ggcctttttt ctccaacaac
     7381 ttcaacaaga cataatctac atgctccatt tggaataagt tctggatgat tgcataaatt
     7441 tggtatttca attccatttt cttttgcagt ttgtaaaatt gttttcccta tttcagtaaa
     7501 tatttctttt ccatctattt caattttgat ttttttattt tccatttcta atccttctca
     7561 acagcaccaa atttacaaat attaaaacat attccacatt taatacattt ttcaacattt
     7621 attttatgtg gcttcttttt ctctccttca atcgcattaa ctggacaatt ttttgcacat
     7681 aaagtacaac cagtacattt ttcaggattt atataatatt ttattaaatt cacacaaaca
     7741 cctgaagaac attttttttc ctttatatga gaaatatatt catctttgaa atatttaatt
     7801 gttgtcaaaa caggatttgg tgctgtttgt ccaagaccac ataaacttgt ttccttaata
     7861 taatttccaa gttcttcaag tttttcaatg tcttctatgt ttgcttttcc ttctgttatt
     7921 ttttcaatta tctcaagcat cctttttgtc ccaattctac atggtacgca ttttccacat
     7981 gactctttct gaacaaaatc aaggaaatat cttgcaagtt caacaatgca taccttatca
     8041 tcaattacaa tcattccgcc tgaacccata attgaaccaa gatttgcaag tgtttcataa
     8101 tcaacaggtg tattgaaaag ttcaacagga atacatcctc ctgatggtcc acctgtttga
     8161 actcctttta tcttccttcc ttcaggcaca ccaccaccaa tttcataaat aattttccat
     8221 aatggtgttc caagaggtat ttcaacaagt ccagtatttt ttatctttcc agcaagtgaa
     8281 aatatttttg ttccaccact tttttctgtt ccaatctttg aaaaattttc tcctctttct
     8341 tcaattatta ttggaatatt tgcaaatgtc tcaacattat ttatacatgt tggttttcca
     8401 aaaagtcctt tttctgctgg aaatggtggt cttggtcttg gcattcccct tttcccttca
     8461 attgaagcaa taagtgcggt ttcctctcca catacaaaag cacctgctcc ttttttaagt
     8521 tcaatatcaa aagaaaaatc tgtcccaaga atgtttttgc caagaattcc gtattcgtaa
     8581 cagattctca atgctttctc aatatgttca atagcaatag gatattctgc cctcacataa
     8641 aaataaccct ttttacaacc agttgtataa gcacctataa gcatcccttc aataacttga
     8701 aatggaactc cttcaagcaa agtcctatcc ataaaagcac cagggtctcc ctcatctcca
     8761 ttacatatta aatatttctc atcatcttgt ttatttttca caatctccca tttcagatat
     8821 gttggaaaac cagcaccacc tcttcccctt aactttgatt tttttaattc ttcaataact
     8881 ttttctggag tatattcatt caggactttt aaaaatgaag aaaaaccgcc attttttata
     8941 tattcatcaa tatcaagtgg gtcaatctca ccacataatt tcaaaacttt aaatgtctga
     9001 ttttttaaaa atttctcatc cttaataacc agttcttcaa ctacctcttt tttttctgcc
     9061 ttttttataa tactttcaac atcttttaaa gagacttttt catataaaaa tgatttccca
     9121 tcaaaactta cagaaacaac aggacctcct gaacagaatt tttgacaacc agttggaatt
     9181 aattttaatt tttttgattc ttttattttc tctttaaaat tttcataaat atcaagtcca
     9241 ccagcagccc tacaaccagt tatacatacc tttatcttta ctctttcatc tttttctttt
     9301 ttcaattttt cacaataatt tttaaaatca gaaaaatttt taaatttcat tttaattttt
     9361 caagtatttt tttaaattta ttctcatcca tatttccata ataacttgaa tttatcatac
     9421 aaacaggagc aagaaaacaa cttccaagac atgcaacaac ctgtaaagta aattttttat
     9481 cctctgttgt cttcccattt tcaagtttaa attccctttt aagcatttca ataattttac
     9541 tacctccttt tacatgacat gctgtcccat cacatactct tatcaagtac tttcctcttt
     9601 cctctgttga aaattgggaa tagaatgtta gaattccaaa aattttactt cttggtattt
     9661 caatttcctc acatattttt ttcaatgttt caataggtac ataattatac ttttcctgaa
     9721 gatattgcaa tatttcaaga tttgagaaat ttttattttc aatttcttca ataatctctt
     9781 ttatttcatt catgtggaaa atcccttacc atttcaatta gatgaactaa aaaatccttc
     9841 aaaacttcaa aacattcctt aacaccattt aaacttcctg gtaaatttat tataacagtt
     9901 tctcccttta ttccacaaac tccccttgaa attacagaaa attttgattt ttcatatgtc
     9961 tttatcctta aaatttcaga aacaccagga atttcttttt caataacttc ttttgttgct
    10021 tcaggtgtta tatctctttt tgttattcca gtgcctcctg ttgttaaaat caaatcacaa
    10081 acttcagaaa attcattcaa cttatttttt atcatctctt tttcatctgc tactatatca
    10141 tattttaaaa catcccatcc aagttcttta caaatttctt ttaagtattt accactttta
    10201 tcctctcttt ctcctttaaa acatccatca cttatcgtta aaatacctat atatattctc
    10261 ggtataacct caataatatc tccttttttt atctcccctc ctttaattac ttttccaaat
    10321 actccttctt tcggcatcaa acatttccca acttttttat aaatttcaca ccaattatgg
    10381 cattttttcc ctatttcaga aatctcaatg acaacatcat taattttaag tttatctcca
    10441 attttaagtt tccttaaatc aatctttcca gttgttatat tctctgcaaa aactccagga
    10501 cttaaaccag tttccttttt caaacagaaa aattcctcat tcatcctttc ccatgataat
    10561 aaacttacct gcctatttat tcctatttct gcatgacaat cacctttaac tccataattc
    10621 tctataagtt caatcttttc aacaggtctt ttgccttctc cctttttttc accaatattt
    10681 acagaataaa caatgccttt catttttgtt ttgagagtaa ttttatatct gttattttca
    10741 tttctttttt aaagccctta cacatatcat atattgtcag aagtgaaaca caaacaccat
    10801 aaagtgcttc catttcaaat ccagtcctat caataccttc aacttcacat ataaccctta
    10861 ttttatcatt ttctatctca aaatttatct ttgcttttgt tatttttact ggatggcaat
    10921 gtggaattgt ataaggagta ttttttattg caaaaagtcc agcactttca gcaaaatcaa
    10981 agacattacc ttttgggact ttattatttt ttattttatc tataacttct ggagcaacaa
    11041 taataaatcc ttctgcaact gctttccttt ttgtaatttt tttatctcca acatctatca
    11101 ttttatcctt tctttatgtg tataaatatt aaatcttttc cctcttgcaa aaccaattat
    11161 tgtaatacca aattcttcac caattcttat tgcataatca gttggtgcac cttttgataa
    11221 aataactgga atcttcattt ttactaattt aaaaataatt tctgaagtaa ttcttccaga
    11281 tgttaaaagt attttatcat ttaaggatat attttttaaa attgcctcac caataacttt
    11341 atcaaaagca ttgtgtctac ttatatcttc tttaaaaata ataatttctc tatcttttgc
    11401 aattccacat acatgaacac aacctgtctt tttatataat tgggaaatcc tattgaaaac
    11461 ttttaaaaga taaaaaatgg catcactttt aaaaaaatta tcaaaattaa ccttatccat
    11521 gatgtttaaa gtaatatgcc tactaccaca ttcagaactt ataaaccaaa aaggacccaa
    11581 aaaatcattt tttttcaatc tcacattaca aatacatcct gaaatatcaa tattttcaat
    11641 ttcttttaaa tctttcacaa ttccttgagt aaataagaaa ccatatatca aataatctat
    11701 attttcaaaa agacaactta aaacaactac ctctttccca tttataaaaa tcctgacata
    11761 cttttcttca acaacctcat cttctaattt attttctccc aaaccttcgg taaatttaac
    11821 tatttcaact ttttttatca ttatccacca actatattca tatatggcag tttataataa
    11881 ctcctttgaa tttctaaaaa tggattataa ctaaattttc tgttgaaaat caaagaaaaa
    11941 ataataccta aaatttcatt ttctttatta ttaaaatttt taaaattaaa aatcggttct
    12001 ccatataaac agctgaatag attaccttta cagtcaatcc ttaacctatc acaattttta
    12061 caaaatggct cacttacagg tgtaacaaat tcaatataaa ttgttttgtc tttgttttca
    12121 tattttttaa ttctcccatt ttcacaaata gggctcaatt ggatatattt ctttaatatt
    12181 tcttcaacaa aaaaataagg aacaaaattt tccttccaga aagtgtaatt tttgtaaaca
    12241 ggcattaatt ctataaactt aagaataact ttattttctt cagcaaattt taaaaaatca
    12301 tatatctcat caagattaat ttctttcatt aaaactatat tcaactttac tacatcaaaa
    12361 ttttttacac tttcttttat accacaaatt attttttcaa gtcctttaac agatgtaatt
    12421 ccttcaaact tctcactctt taaagtatcc aaacttatat ttactctttt tacgcctact
    12481 ttttttaatc tttcaacatt ttcacaaaga aaatatccat ttgttgtaat agatacttct
    12541 tttattccat caatttttga aattttattt atcaagtaat atatgttttt tttaataagt
    12601 ggttctcctc cagtaatttt aatttttttt atcccaaaat ttgtgaaaaa tttcacaata
    12661 tcaacgattt cctcatacga caaaatattt tttttgttta atttataaaa ttctttagaa
    12721 caataaagac atctaaaatt acacttatca gttattgaaa tccttaatgt atctactttc
    12781 cttccaaatt tatccttcat tactctccta ttatctttat caaaactctc tttttccttt
    12841 gaccatcaaa ttcaccataa aaaatctgct cccatggtcc taaatcaagt cttccatttg
    12901 taattgcaac aacaacttct cttcccataa tagttctttt taagtgagca tctgcatttg
    12961 tctctccagt tagattatgt ttgtactttg aaattggttg gtgtggtgca agtttttcaa
    13021 gaaattcaac aaaatcagaa tgtagtcctt tttcatcatc atttataaaa acacttgcag
    13081 ttatatgcat tgcattaaca agacaaatcc cctcttttac tccacttttc tttactgctt
    13141 cctcaacttt ctctgtaata tttataaatt caattctatt ttctgtacaa aaccataaat
    13201 attgggtaaa agatttcatt attttatttt atcatctctt cttttttttt caatattttt
    13261 tactaattct tactgcctac cttaaaggtg ggagaatgta ttcgccacta tgagttgctc
    13321 aattacctct ttgcttaaga ctccagacag ttgtttgttc ttctttaaaa tctcttgggt
    13381 gaagtgactt tattttttta gaaactttaa aggaactaat attaagccct tgagagcggg
    13441 taataaaccg ttcaatgtat ctaaaagtaa ctctgtactt ttgaccaatt tctaaatcag
    13501 tagttccttt tgctttttca tgtaagattg ttttctcaag ttctttttta gttttgaaac
    13561 ttatcttttg gttagccatc ttatcccctt tttcccaagt ccaccatttc atctaacatt
    13621 ttgcgggtat ccgatgttag cgagctaatt tctcaaagga aagggcgaag ccgagcggat
    13681 acgcacctgt tatatgaccc gaacgaagtg aaggcaagca accgaaggtt tcgaagcgtc
    13741 ggaggcgaat gcttttaatt ttcttatctt attcatttat taatatacca ccctttttat
    13801 catacatcca tagcgccatt tcaattttcc tcggcgtcat attatgttcc tctgctagag
    13861 ttctgatttt ctctaagtaa tacatataag aaccaaatga tgcataattt agaaagttgc
    13921 ccataatttc tgaaaactca gtatagtccc caaaaccatc agtttccgac attacccaaa
    13981 gaagagctct ccatactcta taatccaaca cgcaataatt attcggaaaa accaccgtta
    14041 aaatggccga ggctactggc acaccaacac cttcaagttg ggttagttta tatatttgcc
    14101 cttctatatt caggtctccg gttatgacta cgtttgttat ctccattata tcttcttcag
    14161 aattttcttg atacctgttt ttttgtcttt tggtcttcca ttcacaaata cttataaact
    14221 cctttttcga tagaaatcct ctttgttgta aaacctgaga aattgattca aaatatggtg
    14281 aatattcatg tgctatttct ccgttaccat ctatgtagta ttgtatagag atgttttttc
    14341 ttctgtaatt tctttcgtat tctgctgccc attcattgaa atctataggc atatttgatc
    14401 cctccaagat ttaccctttt ttatctaaca atcgccctcc gatgtcatat aatgtttcca
    14461 gcataaaaga cgtttttgcg aagcaaaaat ttgggtaagt gctgtaagac acgacccttt
    14521 tatgttgtgt tattgtaagt tgcatattcg aatatttcat attcctttgt ctct
//
LOCUS       DAOSAT010000045        14534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2814_length_14534_cov_4.632088, whole genome shotgun sequence.
ACCESSION   DAOSAT010000045 DAOSAT010000000
VERSION     DAOSAT010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14534
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2814_length_14534_cov_4.632088"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..433
                     /gene="rpsJ"
                     /locus_tag="PLF90_05070"
     CDS             122..433
                     /gene="rpsJ"
                     /locus_tag="PLF90_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOK56682.1"
                     /translation="MDGKRIRIKLKSFDHRLLDRSVKEIIEIVRGTGVRINGPVPLPT
                     KREIYTVLRSPHVNKKSREQFELKIHKRLIDIIDPTPRTVDALSKLSLPAGVEVEMKE
                     S"
     gene            442..1086
                     /gene="rplC"
                     /locus_tag="PLF90_05075"
     CDS             442..1086
                     /gene="rplC"
                     /locus_tag="PLF90_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOK56683.1"
                     /translation="MGIGIVGKKLKMTQVFTDDGEMIPVTLIEYREGYVVDLKTEEKD
                     GYNAIKVGFEEVSEKKLNKPLLGYLKKRNLPPLKILKEFRFENKEDMKDFKIGERIKI
                     DTIFKEGDYVDVTGKSIGKGFQGVVKRHGFHGGPATHGSMSHRAPGSIGSTAPQRVVK
                     GRKMAGHMGTETVTVQNLQIFKILNEENLIMVKGAVPGPSNSYLILKKAIKKRR"
     gene            1090..1737
                     /gene="rplD"
                     /locus_tag="PLF90_05080"
     CDS             1090..1737
                     /gene="rplD"
                     /locus_tag="PLF90_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOK56684.1"
                     /translation="MVKIGVYDIEGNLIEEREISENIVKEPINKDVLYYYTVSYLNNQ
                     RLGTHSTKTRGEVSGGGRKPWRQKGTGRARVGSIRNPIWRHGGIAFGPKPGDYYIRLP
                     RKIRRKALFESIRDKIIENRVIFLKTGNIEKPKTKIFSNFLKKIGYENEKVLFVFKNT
                     EEKKNKILSVRNLVLAKYDYINRINAYEILNSDRLIFEEDCFENLKNFLEGGKIV"
     gene            1730..2011
                     /gene="rplW"
                     /locus_tag="PLF90_05085"
     CDS             1730..2011
                     /gene="rplW"
                     /locus_tag="PLF90_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOK56685.1"
                     /translation="MFDPYKIVKNPKITEKGTELNKENKYVFCVDRKANKNQIKKAIE
                     EIYKVKVEKIYVINVKPKKRRYRFYIEGYKSGYKKAIVKLKEGEKIAIT"
     gene            1998..2822
                     /gene="rplB"
                     /locus_tag="PLF90_05090"
     CDS             1998..2822
                     /gene="rplB"
                     /locus_tag="PLF90_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOK56686.1"
                     /translation="MPLRKLKPVTPGQRHALLQDFSDITKTEPEKSLVVGLKKTGGRN
                     NTGRITTRHIGGGHKRLYRIIDFKGRINHEGIVKSIEYDPNRNARIALIHYTDGVKSY
                     IIAPLGLSIGDRIKCGEDAEIKVGNRLPLKKIPEGVQIYNIELIPGKGGKLVRSAGTL
                     ATLMVKGEKYAQVRLPSGEVRLFDLNCYASIGQVSNPEYKYISFGKAGRVRNLGIRPT
                     VRATAMNPVDHPMGGGEGRGKGHQSQSPTGVPAKGYKTRKKKKPSDKLIIQRRKQK"
     gene            2819..3100
                     /gene="rpsS"
                     /locus_tag="PLF90_05095"
     CDS             2819..3100
                     /gene="rpsS"
                     /locus_tag="PLF90_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOK56687.1"
                     /translation="MRSKKKGPYIDKKLMEKVLKQKESGKKEVIRTWARSCTIVPEFV
                     GHTFGVHNGRTFVNVYVTENMVGHRLGEFAPTRTFRGHGAHTERAREKK"
     gene            3113..3448
                     /gene="rplV"
                     /locus_tag="PLF90_05100"
     CDS             3113..3448
                     /gene="rplV"
                     /locus_tag="PLF90_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOK56688.1"
                     /translation="MEVVAKAKYVRIGPRKLRVLVELVKGKKVEEAFGILRNVKKRGA
                     KILEKVIKSAENNGKNKGEGIEWKIKNIIIDGGPMLKRYRAATMGRGVMIRKRTSHIT
                     VILEGKGEE"
     gene            3448..4104
                     /gene="rpsC"
                     /locus_tag="PLF90_05105"
     CDS             3448..4104
                     /gene="rpsC"
                     /locus_tag="PLF90_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOK56689.1"
                     /translation="MGQKVNPIGLRIGIVEQWRSKWFSSKKEFKNLLREDVIIRDYIR
                     KRYPRGTITKIEIEKTDKIRIRLYTPRPGVVLGRKGAEINQLRDELYEILKKQITIDC
                     IEINPPGTSAQFLADSIVIQLEKRVPHRQAIKKAMDLAIQSGAKGIKVRISGRIGGVE
                     IARSEQYILGKVSLHTLRAKIDYATSTAFTRSGTVGVKVWVYLGEILEEKGEKNAVNA
                     "
     gene            4088..4507
                     /gene="rplP"
                     /locus_tag="PLF90_05110"
     CDS             4088..4507
                     /gene="rplP"
                     /locus_tag="PLF90_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOK56690.1"
                     /translation="MPLMPKRVKYRKMQRGRTKGQAERGNKLFYGEYGLKVLEPGWIT
                     SAQIEAARIAITHYLKSKGKLWIRIFPDKPVTKKPAESRMGKGKGDVEKWVAVVLPGK
                     IIFEIAGVSDELAKEALRRASYKIPYKSKIVTRSLVS"
     gene            4570..4794
                     /gene="rpmC"
                     /locus_tag="PLF90_05115"
     CDS             4570..4794
                     /gene="rpmC"
                     /locus_tag="PLF90_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOK56691.1"
                     /translation="MKKEELNKWKEATKEEIGNRILELKKQLYELNHQLLLGQLKNYS
                     QIKEIKKDIARLKTILREKEIGAKNVKKNN"
     gene            4775..5065
                     /gene="rpsQ"
                     /locus_tag="PLF90_05120"
     CDS             4775..5065
                     /gene="rpsQ"
                     /locus_tag="PLF90_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOK56692.1"
                     /translation="MSKRTIEKTGVVVSDKMDKTRVVMVEYMTQHPIYKKILRKRRKF
                     MVHDEDNITKVGDVVRIIQTRPLSKNKRWKIIEIIGKKGVISDSTEVNASGS"
     gene            5031..5399
                     /gene="rplN"
                     /locus_tag="PLF90_05125"
     CDS             5031..5399
                     /gene="rplN"
                     /locus_tag="PLF90_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOK56693.1"
                     /translation="MIQLRSMLQVVDNTGAKKIMVIGFIGSSKKRYGYVGDVVVATVK
                     HALPDGIVKKKEKVKAVIVRTKKETRRKDGSILKFDHNCAVIIDNEGNPRGTRVFGPV
                     PRELREKNFTKIISLAPEVI"
     gene            5401..5724
                     /gene="rplX"
                     /locus_tag="PLF90_05130"
     CDS             5401..5724
                     /gene="rplX"
                     /locus_tag="PLF90_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOK56694.1"
                     /translation="MKFKLKRGDIVQVIKGKDRGKKGRILKIFKEENKVLVEGINFVK
                     KHTRPRSVDRQGGIIQMEKPIAISNVNFFCLKCSKPTRLGIKFLEDGTKVRFCKKCKE
                     IVEEK"
     gene            5726..6274
                     /gene="rplE"
                     /locus_tag="PLF90_05135"
     CDS             5726..6274
                     /gene="rplE"
                     /locus_tag="PLF90_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017748881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOK56695.1"
                     /translation="MEEPRLKKDYREKVVPELLKRFNYKNKMQVPKILKIVLNMGIGR
                     DNKDAKAIESAQKELTLIAGQKAVLTKAKKSIAGFNVRKGNIVGVMVTLRGNRMYEFL
                     DRLITVALPRVRDFEGLPGKSTDGKGSYTIGIKEHVIFPEVDYNTIYKVQGLNVTIVT
                     SAKTPEETISLLREIGLPIREE"
     gene            6279..6464
                     /locus_tag="PLF90_05140"
     CDS             6279..6464
                     /locus_tag="PLF90_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOK56696.1"
                     /translation="MARKCFFEKVKRGAKFKVRERNRCWRCGRPRGYYRKFGICRVCF
                     RELASKGEIPGVKKASW"
     gene            6480..6875
                     /gene="rpsH"
                     /locus_tag="PLF90_05145"
     CDS             6480..6875
                     /gene="rpsH"
                     /locus_tag="PLF90_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOK56697.1"
                     /translation="MTMTDPIADMLTRIRNANAISKSYVDVPFSKFKMKILDVLKEEK
                     YIKDYELIQDNSRKYIRVHLLYFGKNKAITEIKRISTPGRKFYVSKDKIPVVKNNYGI
                     AVLTTSKGVMSNKKAKEIGIGGEILFYIW"
     gene            6888..7454
                     /gene="rplF"
                     /locus_tag="PLF90_05150"
     CDS             6888..7454
                     /gene="rplF"
                     /locus_tag="PLF90_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOK56698.1"
                     /translation="MARLGKKPIIIPEGVKVKIENGKVYVEGKKGNLVQEIFPNLSVV
                     VEDKKIIVKNEVDPKNKKLYRKTNALWGLLRSLIFNMVKGVNEGFEKILEIHGVGYKG
                     EVKGNKLILSVGFTHPVEVEIPKELSVEVSRNTIIFVRGADKQKVGEFAASIRRICPP
                     EPYKGKGIRYRGEYVRQKATKAGVGATK"
     gene            7451..7831
                     /gene="rplR"
                     /locus_tag="PLF90_05155"
     CDS             7451..7831
                     /gene="rplR"
                     /locus_tag="PLF90_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015161728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOK56699.1"
                     /translation="MKLTRKEKRIKRHIRVRKKVKGSSDRPRLCIYKSLKHIYASLID
                     DSVTPNKVITTVSSLSPEFKKLATDKSIHGGNLKGAELVGKLVAEKAISLGIEKVVFD
                     RGGYKYTGRIKVLAESARKGGLKF"
     gene            7825..8301
                     /gene="rpsE"
                     /locus_tag="PLF90_05160"
     CDS             7825..8301
                     /gene="rpsE"
                     /locus_tag="PLF90_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOK56700.1"
                     /translation="MLEKENLLGETEEKQIVIEIRRVTKVTKGGKNLGFRAIVVVGDG
                     KGRAGFGVGKANEVPEAIRKAVGQARKNMIEVPLKEGTIPHDVESKFGPSRVILKPAS
                     KGHGMVAGRTIRALFDVCGIKDVTCKCLGSTNPINVLHATVKALSKIKIRSEENET"
     gene            8291..8731
                     /gene="rplO"
                     /locus_tag="PLF90_05165"
     CDS             8291..8731
                     /gene="rplO"
                     /locus_tag="PLF90_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOK56701.1"
                     /translation="MKLNELKPPSGAKHKKKRLGRGDSSGHGGTSTRGHKGHKARSGY
                     KLPFNFEGGQMPLFRRLPKRGFTHLKEYKIEIVNLSQIEKLFNDGEEITIEKLKEKGL
                     IKKGEFVKILGEGNLTKKLSFFAHKFSKKAKEKIEKVGGKINII"
     gene            8743..10056
                     /gene="secY"
                     /locus_tag="PLF90_05170"
     CDS             8743..10056
                     /gene="secY"
                     /locus_tag="PLF90_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOK56702.1"
                     /translation="MFEAFRDTFKIPDLKKRIVITILLLTVYRFGCYIPVPGINGKEL
                     AKIFESSAGTLFSLADLFSGGALSQATIFALGIMPYISVSIVLELLASVVPYFENMLR
                     SGVEGRRKLTQLTRYGTVGLCIFQGMAISSFLENPEHFGGLVIVANPGWLFRIITTLT
                     LTTGTIFLMYLGEQITEKGIGNGISLIITASILSRMPVAFHRMAGLVSAGEINFFVVI
                     ILIALIFGVVAAAILINESERRIPIQYAKRVIGKRIYGGQSTYLPIKLNQGGVIPLIF
                     AIAILTFPATILSFAQNKFLIKLSTILSPQQPVGMFLYAILTIFFCYFYAGIIFNPDN
                     IAEDLQKYGGFIQGIRPGKSTALYLEKVLNRLTLPGSLMLAGIALFPYILMHIYRKIP
                     YIVASIFGGIGLLIVVSVLIETIRQIEAHLLLRHYQGFIKRAKIK"
     gene            10053..10709
                     /locus_tag="PLF90_05175"
     CDS             10053..10709
                     /locus_tag="PLF90_05175"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOK56703.1"
                     /translation="MKEIIILLGAPGAGKGTVGERLSKIYNIPVVSTGDLLRENVKAK
                     TELGLKAKEYMDRGELVPDEIVISILAERIKKNDCKNGFILDGFPRNINQARKLEDYI
                     KEEKVKVVYLKADDEFLVKRLSNRRICETCGAIYHLINIPPKVPGVCDKCGGKLIQRE
                     DDKEEVVRNRLVVYHNLTAPLLEYYKKKGILFTVPGDGKLEEIINQIINLKNNGEKGN
                     "
     gene            10711..11421
                     /gene="map"
                     /locus_tag="PLF90_05180"
     CDS             10711..11421
                     /gene="map"
                     /locus_tag="PLF90_05180"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOK56704.1"
                     /translation="MVRIACKKAREIIEELKEYLVEGISTYEIELKSIEMMKKKNVES
                     AFKGYRGYPANICISINHEVVHGIPKKGKILKNGDIVSIDVGVKYNGFYGDCADTFPV
                     GKVDVKYIKLIEVTKKAFLKSLKYVYAGSKTGSIGSTIEEYVKRHGFSVIRDFTGHGI
                     GKNLHESPEIPNFGEKGKGDILLENRIIAIEPMIAEGTGEIEILEDGWTAVTKDRGYA
                     AHYENCVLIKKDGYEVLS"
     gene            11424..11639
                     /gene="infA"
                     /locus_tag="PLF90_05185"
     CDS             11424..11639
                     /gene="infA"
                     /locus_tag="PLF90_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013755893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOK56705.1"
                     /translation="MAKEEKIKMEGVIKKVLPATTFEVELENGHEIIAHLCGKMRMNY
                     IKIVPGDKVIVEISPYDLKRGRIIKRI"
     gene            11653..11778
                     /gene="rpmJ"
                     /locus_tag="PLF90_05190"
     CDS             11653..11778
                     /gene="rpmJ"
                     /locus_tag="PLF90_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOK56706.1"
                     /translation="MKVRASVRKICPKCKIIKRKGIVRVICSNPQDGPKHKQRQG"
     gene            11791..12201
                     /gene="rpsM"
                     /locus_tag="PLF90_05195"
     CDS             11791..12201
                     /gene="rpsM"
                     /locus_tag="PLF90_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOK56707.1"
                     /translation="MARILGVEIPDNKKVKIALMYIYGIGDKRAEEIVKNTGVNGDKR
                     VKELTEEELGKITSFIQKNYKIEGDLKQEIAQNVRRLIEINCYRGIRHKLSLPVRGQR
                     TRSNARTRKGPRRTVGVVRDKAARKAIKQQKEEK"
     gene            12213..12602
                     /gene="rpsK"
                     /locus_tag="PLF90_05200"
     CDS             12213..12602
                     /gene="rpsK"
                     /locus_tag="PLF90_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOK56708.1"
                     /translation="MPRKYIKKRKKKITVPKGIAHIKATFNNTIITITDPEGHVLLWG
                     SAGTSGFKGTRKGTPFAAQLAAENIAKRAQTMFNMKEVDVKVKGPGPGRETAIRALQS
                     AGLNVVSIKDVTPIPHNGCRPPKRRRV"
     gene            12621..13241
                     /gene="rpsD"
                     /locus_tag="PLF90_05205"
     CDS             12621..13241
                     /gene="rpsD"
                     /locus_tag="PLF90_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOK56709.1"
                     /translation="MAKYTKPLCKICRKYGIKLYLKGRRCESDKCAVDQRKGKIRLIK
                     QKKMSEYGLQLREKNKVKIFYGVMERQFKRYFEIAKKHKGVTGEVLLQLLERRLDNVV
                     FKANWVHSRKMAKQLINHGHIIINGRRVDRPGYLVEIGDIIQVKPNSKYIKTVKECIE
                     EYKTRTRPLWLKVDDENFKIEILRYPDRSEVSIPVEEQLIINLYSK"
     gene            13260..14237
                     /locus_tag="PLF90_05210"
     CDS             13260..14237
                     /locus_tag="PLF90_05210"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOK56710.1"
                     /translation="MKEFIYPSKFLWEKETLRKNYGKLIIEPLERGYGVTIGNALRRV
                     LLSSIPGVAITGLRIKGVLHEFATLEGMKEDIVEVVLNVKQIALKPIINEYPHTVSVE
                     ISDKEEICAGDLVNDGSVEVINKDLHIATVDPSKKYQFEIDITKGFGYLPIEKMKLMI
                     KDLPVGTILLDGLYSPVRKVTFHVENTRVEQFVDYEKLILEVWTTGAVTPEESVNYAC
                     ELLEKQFELIIKGQKIEEEEEEITEKKEIKDELDIPITDLKLSTRVYNALENKNIKTL
                     RDLLNTPIETLEEMKNLGKKSLEEIEKALKKRGYRLKSSSELAQTKESK"
     gene            14234..14524
                     /gene="rplQ"
                     /locus_tag="PLF90_05215"
     CDS             14234..14524
                     /gene="rplQ"
                     /locus_tag="PLF90_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOK56711.1"
                     /translation="MRHKKDIKKLGRNKSHRKALMRNLTISFFQNQKIVTTVPKAKQL
                     RRIVERLITLAKKGTLADIRRINQFLNHPQTVKKIIDVSKDFLIEMVDIPRL"
BASE COUNT         5970 a         1492 c         3037 g         4035 t
ORIGIN      
        1 agagaaggag ggaagacagt aggtgctggt gctgttactg aaataattga ataaaaaatt
       61 tgcattgaat aaaaaaattt gatataatgt tttttttaca aataattaat ggaaaagaaa
      121 aatggatgga aaaagaatta gaataaaatt aaagtctttt gaccacaggt tgctggacag
      181 gtcagtgaaa gagataattg agattgtaag gggtacaggt gtcagaataa atggacctgt
      241 tcctttacca acaaaaagag agatttatac tgttttaaga tctccacatg taaataaaaa
      301 aagtagagaa cagtttgaac tgaaaataca taaacgactt atagatataa ttgatccgac
      361 accaagaact gttgatgctt taagtaaatt aagtttacca gctggagttg aagtagaaat
      421 gaaggaaagt tgaggaataa aatgggaata ggaattgttg gaaaaaaact taaaatgaca
      481 caggttttta ctgatgatgg agaaatgatt cccgttactt taattgagta cagagaaggt
      541 tatgttgttg atttaaaaac agaagaaaaa gatggttata atgctataaa ggttggtttt
      601 gaagaagtaa gtgaaaaaaa attaaacaaa cccttacttg gatatttaaa gaaaagaaat
      661 cttcctcctt taaaaatttt aaaagaattt aggtttgaaa ataaagaaga tatgaaagat
      721 tttaaaattg gagaaagaat aaaaatagat actattttta aagaaggtga ttatgttgat
      781 gttacgggta aatcaatagg taaaggtttt caaggagtag taaagagaca tggttttcat
      841 ggtggtccgg caacgcatgg ttctatgtct catagagcgc ctggttcaat tggttcaact
      901 gctcctcaaa gggttgtaaa aggtagaaaa atggcaggac atatgggaac tgaaacagtt
      961 acagttcaaa atttacagat atttaaaata ttaaatgagg aaaatttaat tatggtaaaa
     1021 ggtgcagttc ccggtccatc aaattcatat ttaattttaa aaaaagcaat taaaaaaaga
     1081 aggtaaaaaa tggttaaaat aggggtttat gatattgaag gtaatttaat tgaagaaaga
     1141 gagatatctg aaaatatcgt gaaagaacca attaataaag atgttttgta ttattatact
     1201 gtgagttatt taaacaatca gcgacttgga actcattcaa caaaaacaag aggtgaagta
     1261 agtggtggag gcaggaaacc gtggagacag aaaggtaccg gtagagcaag ggttggttca
     1321 ataaggaatc ctatatggag acatggaggg attgcttttg gtccaaaacc aggagattat
     1381 tatataagat tacccagaaa aattagaagg aaagcattat ttgaatcaat cagagataaa
     1441 attattgaaa acagagtaat atttttaaaa acagggaata ttgaaaaacc aaagacaaaa
     1501 attttcagta attttttaaa gaaaattggt tatgagaatg aaaaagtcct gtttgttttt
     1561 aaaaatactg aagagaaaaa aaataaaatt ttatctgtaa gaaatttagt tcttgcaaaa
     1621 tatgattata ttaataggat aaacgcttat gaaattttaa acagcgatag gttgattttt
     1681 gaagaagatt gttttgaaaa tttaaaaaat tttctggaag gagggaaaat tgtttgaccc
     1741 ttataaaatt gtaaaaaatc caaaaataac agaaaaaggt acagagttaa acaaagagaa
     1801 taaatatgtc ttctgtgttg acagaaaagc aaataagaac cagataaaaa aggcaataga
     1861 agaaatttat aaggtaaaag tggaaaaaat ttatgttata aatgtaaaac ctaaaaaaag
     1921 aagatataga ttttatattg aaggatataa atcaggatat aaaaaagcaa ttgttaaatt
     1981 gaaagaagga gaaaaaattg ccattacgta aattaaaacc ggttactcct ggtcagagac
     2041 atgctttatt acaggacttt tctgatatta ctaaaacaga accagaaaag tccctggtcg
     2101 ttggtttgaa aaagacagga ggaagaaata atacaggtcg tattactacc agacatattg
     2161 gaggaggaca taaaagatta tatagaatta ttgattttaa gggaagaatt aatcacgaag
     2221 gcatagttaa gagtattgaa tatgacccaa atagaaatgc aagaattgct ctgattcatt
     2281 atacagatgg tgtaaaaagt tatattatcg caccattagg actttcaata ggagacagaa
     2341 ttaaatgtgg agaggatgca gaaataaaag tcggaaatag attacctttg aaaaaaatac
     2401 ctgaaggtgt tcaaatatat aatattgaac ttatacccgg gaaaggaggt aagttggtca
     2461 ggagtgcagg gactcttgca acattgatgg taaagggaga aaagtatgct caggtaagat
     2521 taccatctgg tgaagtcaga ttgtttgacc ttaactgtta tgcatctatt ggtcaggtta
     2581 gtaatccaga atataaatat atatcatttg gtaaagcagg aagggtgagg aatctgggta
     2641 taagaccaac tgtaagagca acagcaatga atcctgttga ccatcctatg ggtggtggag
     2701 aaggaagagg gaaaggccat cagtcacaga gtccaacagg ggttcctgca aaaggatata
     2761 aaacaagaaa aaagaaaaaa ccatcagata aactaataat acagagaagg aaacaaaaat
     2821 gaggtcaaag aaaaaaggtc catatattga caaaaaactt atggaaaaag ttttgaagca
     2881 aaaagaatcg ggtaaaaaag aagttataag aacttgggca agaagttgta caattgttcc
     2941 tgaatttgtt gggcatactt ttggagttca taatggaagg acttttgtta atgtctatgt
     3001 aacagaaaat atggttggtc atcgtctggg agaatttgca ccgaccagaa cctttagagg
     3061 acatggtgct catacagaaa gagcgagaga aaagaaataa aggggattaa aaatggaagt
     3121 agtagcaaaa gcaaagtatg ttagaatagg tccaagaaaa ttaagagttc ttgttgaact
     3181 tgttaaaggt aaaaaagttg aagaagcatt tggaattttg agaaatgtaa aaaaaagagg
     3241 agcaaaaatt cttgaaaagg ttattaaaag tgcagagaat aatggtaaaa ataaaggtga
     3301 aggaattgaa tggaaaataa aaaatattat aattgatgga ggtccaatgt taaaaagata
     3361 tagagcagca actatgggaa gaggggttat gattagaaaa aggacatcac atattactgt
     3421 tatacttgaa ggaaaaggag aagaataatg ggacaaaagg ttaatcctat tggattaaga
     3481 attggtatag ttgaacagtg gagaagtaaa tggttcagtt caaaaaaaga atttaagaat
     3541 ttattgagag aagatgttat aataagagat tatataagaa agagatatcc aagaggtaca
     3601 attactaaaa ttgaaataga gaaaacagat aaaataagaa tcagattgta cacaccaaga
     3661 cccggtgttg ttcttggtag aaagggtgca gaaattaatc aattaagaga tgaattatat
     3721 gaaattttaa aaaaacagat tactattgac tgcatagaaa ttaatccacc aggaacaagt
     3781 gcacaatttc ttgctgactc aattgttata caacttgaaa agagagttcc tcacagacaa
     3841 gcaattaaaa aagcaatgga ccttgcaatt cagtcaggtg caaaaggaat aaaggtgaga
     3901 atttcaggaa gaattggagg tgtagaaata gcaagaagtg aacaatatat acttggtaag
     3961 gtttctcttc atacattaag agcaaagatt gattatgcaa caagtactgc ttttacaaga
     4021 agtggaacag taggtgtaaa agtttgggtt tatcttggag aaattttgga agaaaaagga
     4081 gaaaaaaatg ccgttaatgc ctaaaagagt taaatacaga aaaatgcaga gggggagaac
     4141 aaaaggacag gcggaaagag gtaataaact tttttatgga gagtatggtt tgaaagttct
     4201 tgaaccggga tggataactt ctgctcaaat agaagcagca agaatagcaa taacccatta
     4261 tttaaaatca aaaggtaaac tctggataag aatttttcct gataaaccag tgactaaaaa
     4321 acctgctgaa agtagaatgg gaaaaggcaa gggtgatgtt gaaaaatggg ttgctgttgt
     4381 tttacccggt aaaataattt ttgaaattgc aggtgtttca gatgaacttg caaaagaagc
     4441 attaagaaga gcatcctata aaatcccata taaatcaaag attgttacaa ggtcacttgt
     4501 aagttaaaaa tttgaaattt ttaaagaaaa tgctataata ttattttatg tttttaaaaa
     4561 gggaataaaa tgaaaaaaga agaattaaat aagtggaagg aagcaacaaa agaagaaata
     4621 ggaaatagaa tcttagaatt gaaaaaacaa ctttatgagt taaatcatca gttactcttg
     4681 ggacaattaa aaaattactc acagattaaa gagataaaaa aagatatagc aaggttaaag
     4741 accatattaa gagagaaaga aattggagct aaaaatgtca aaaagaacaa ttgaaaaaac
     4801 aggagtagtt gtaagcgata agatggataa aacaagagtt gttatggttg aatatatgac
     4861 acagcatcca atttataaaa aaatattaag gaaaagaaga aagtttatgg tacatgacga
     4921 agataatatt acaaaagttg gagatgtggt tagaattatt caaacaagac ctttaagtaa
     4981 aaataaaaga tggaaaatta ttgaaattat tggtaaaaaa ggagttataa gtgattcaac
     5041 tgaggtcaat gcttcaggta gttgataata caggtgcaaa aaaaataatg gttattggat
     5101 ttataggtag ttcaaagaaa agatatggtt atgttggaga tgttgttgtt gctactgtga
     5161 agcacgctct tcctgatggg atagtaaaaa agaaagaaaa agtcaaggcg gttattgtga
     5221 gaactaaaaa agagacaaga aggaaggatg gttcaatttt aaaatttgac cataattgtg
     5281 cagttataat tgataatgaa ggtaatccga gaggaacaag agtttttggt cctgttccaa
     5341 gggagttgag agaaaagaat tttacaaaga taatttctct tgcaccggag gttatataaa
     5401 atgaaattta agttgaaaag aggagatatt gttcaggtta taaaaggaaa agatagaggg
     5461 aaaaaaggaa gaattttaaa aatttttaaa gaagaaaata aagtacttgt tgagggtatt
     5521 aattttgtta aaaaacacac acgaccgaga tcagtagaca ggcagggagg aataattcaa
     5581 atggaaaaac ccattgcaat aagcaatgtt aattttttct gtttaaaatg cagtaagcca
     5641 acacggctgg gaataaaatt tttagaagat ggtacaaaag taagattttg taaaaaatgt
     5701 aaggagatag tagaggaaaa gtaaaatgga agagccaaga ttaaaaaaag attataggga
     5761 aaaagttgtt cctgaattgt tgaagagatt taattataaa aataaaatgc aggttcctaa
     5821 aattttaaaa atagttttaa atatgggaat aggcagggat aataaagacg ctaaagcaat
     5881 tgaatctgct caaaaagaac ttactttaat cgctggacag aaagctgttt taactaaagc
     5941 aaaaaaatca atagcaggtt ttaatgttag aaaaggaaat attgtaggag ttatggttac
     6001 actaagagga aacagaatgt atgaatttct tgatagattg attactgtgg ctttgccaag
     6061 ggtaagagat tttgaaggat taccaggaaa atcaacagat ggaaaaggtt cgtatactat
     6121 agggataaaa gaacatgtga tatttccaga ggttgattat aatacaattt ataaagttca
     6181 aggactcaat gtcacaatag ttacttcagc aaaaacaccc gaagaaacaa tatctttatt
     6241 aagagaaatt ggtttaccga taagggagga ataaaaaagt ggcgagaaaa tgtttttttg
     6301 aaaaagtaaa aagaggagca aaatttaaag taagagaaag aaatagatgt tggagatgtg
     6361 gaaggccaag gggttattac agaaaatttg gtatttgtag agtatgtttt agagaacttg
     6421 cttcaaaagg agaaatccca ggtgttaaaa aggcaagttg gtaaaggagg agggacttta
     6481 tgacaatgac agaccctatt gcagatatgt taacaaggat tagaaatgca aatgcaatat
     6541 caaagtcata tgttgatgtt cctttcagta agtttaaaat gaaaatactt gatgttctta
     6601 aagaagagaa atatataaag gattacgagt taattcagga taattcaaga aaatatatta
     6661 gagttcatct tttatatttt ggaaaaaata aggcaataac agaaataaaa agaataagta
     6721 ctcctggaag aaaattttat gtttcaaagg ataaaattcc tgttgttaaa aataattatg
     6781 gaatagctgt tttgacaact tcaaaaggag ttatgagtaa taaaaaagca aaggaaatag
     6841 gaatcggagg agaaattctc ttttatatat ggtaaaaagg aaaaaccatg gcaagattag
     6901 gcaaaaaacc aattattatt cctgaaggtg ttaaagtaaa aatagaaaat ggtaaagtgt
     6961 atgttgaggg aaaaaaaggg aatctcgttc aggaaatttt cccaaattta agtgttgtgg
     7021 ttgaggataa aaagattatt gttaaaaacg aggtagaccc aaaaaacaaa aaactatata
     7081 gaaaaaccaa tgctttatgg ggacttctaa ggagtttaat ctttaatatg gtaaaaggtg
     7141 taaatgaagg ttttgaaaaa atacttgaaa ttcatggagt gggatataaa ggagaagtta
     7201 agggcaacaa acttattctt tcagtgggtt ttacccatcc agttgaggtt gaaataccaa
     7261 aagaattatc tgtggaagtg agcagaaata cgataatatt tgtaagagga gcagataagc
     7321 agaaggttgg agaatttgca gcaagtataa gaagaatttg tccacctgaa ccatataaag
     7381 gtaagggaat tagatataga ggagagtatg ttagacaaaa ggcaacaaaa gccggagtag
     7441 gagcaacaaa atgaaactga caagaaaaga aaaaagaata aaaagacata taagagtgag
     7501 aaagaaagta aaaggttctt ctgatagacc aaggttgtgt atttacaaaa gtttaaaaca
     7561 tatatatgct tctttgattg atgatagtgt tacacccaat aaagttatta ctactgtttc
     7621 aagtttatca cctgaattta agaaacttgc tactgataaa agtattcatg gtggaaattt
     7681 gaaaggtgca gaacttgttg gaaaacttgt ggctgaaaaa gccatatcac ttgggataga
     7741 aaaggttgtt tttgataggg gtggatataa atatactggt agaattaaag ttttagctga
     7801 gtctgcaaga aaaggaggtc taaaatttta gaaaaagaaa atttacttgg tgaaacagaa
     7861 gaaaaacaaa ttgtaattga aataagaaga gtgacaaagg ttacaaaagg tgggaaaaat
     7921 ttaggtttta gagcaattgt tgttgttgga gatggtaaag gaagagctgg ttttggagtt
     7981 ggtaaagcaa atgaggttcc agaggcaata aggaaagcag tgggacaggc aagaaagaat
     8041 atgatagaag ttcctttaaa agaaggaacg attccacatg atgtagaaag taaattcggt
     8101 ccttcaagag taattttaaa acctgcttct aaaggacatg gtatggtggc agggaggacc
     8161 ataagagcat tatttgatgt atgtggaata aaagatgtta catgtaaatg tcttggttca
     8221 acaaacccga taaatgtttt acatgctacg gtaaaggcat taagtaaaat aaaaataagg
     8281 agtgaagaaa atgaaactta atgagttaaa accaccttct ggtgcaaaac ataagaaaaa
     8341 acgtctcgga agaggagatt cttccggaca tggaggaact tcaacaagag gacataaagg
     8401 gcataaagca agaagtggtt ataaattacc ttttaatttt gaaggtggac aaatgccttt
     8461 gttcagaaga ttgcctaaga gaggtttcac acatttaaaa gaatataaaa ttgaaatagt
     8521 aaatttaagt cagattgaaa agttatttaa tgatggagaa gaaattacaa tagaaaaatt
     8581 aaaagaaaaa gggctaatta aaaaaggtga atttgtaaaa attcttggag aaggaaattt
     8641 aacaaaaaaa ctttcatttt ttgctcataa gttttcaaaa aaagcaaaag aaaaaataga
     8701 aaaagttggt ggaaagatta acattattta ggagaagtaa aaatgtttga ggcattcaga
     8761 gatacattta aaataccaga tttaaaaaag aggatagtta taacaatctt attgttaaca
     8821 gtttatagat ttggatgtta tatacctgtt ccaggtatta atgggaaaga acttgctaaa
     8881 atatttgaga gttcagcagg tactcttttt tctcttgcag atttattttc tggtggtgca
     8941 ttgagtcagg caactatttt cgctcttggg attatgcctt atataagtgt ttcaattgtt
     9001 cttgaacttc tcgcttctgt tgtgccttat tttgagaata tgttgagaag tggagttgaa
     9061 ggaaggagaa aacttacaca acttacaaga tatggaactg ttggtctttg catttttcag
     9121 ggtatggcta taagttcttt ccttgaaaac cctgaacatt ttggagggct tgtaatagtt
     9181 gcaaatcctg gatggttatt tcgtattata acaactctta cattgacaac aggaacaatc
     9241 tttcttatgt atcttggtga acagataaca gagaaaggta taggtaacgg aatttctttg
     9301 ataattactg caagtatttt gtcaagaatg cctgttgcat ttcatagaat ggctggactt
     9361 gtaagtgcag gagaaataaa tttttttgtt gttattatac tgattgcctt aatttttgga
     9421 gtagttgcag cagctatttt aataaatgaa agtgaaagaa ggataccaat acaatatgct
     9481 aaaagagtga taggtaaaag aatttatgga ggtcagtcaa cctatcttcc tataaagtta
     9541 aatcagggtg gagtaattcc tttaattttt gcaatagcca ttttaacttt tcctgcaaca
     9601 attctttctt ttgcacagaa taaattttta attaaattat caactatttt atctcctcaa
     9661 caacctgttg gtatgttttt atatgctatt ttaacaatat ttttctgtta tttttatgct
     9721 ggaattattt ttaatccgga taatattgcg gaagaccttc agaaatatgg cggttttata
     9781 cagggtataa gacctggaaa atcaactgcc ctttatcttg aaaaggttct gaatagatta
     9841 actctacccg gttctttaat gcttgcaggt attgcacttt ttccctatat acttatgcat
     9901 atttacagaa aaatacctta cattgttgca agtatatttg gtggaatagg tcttcttata
     9961 gttgtttctg ttttaattga aacaataaga cagattgaag cacatttact tttaagacat
    10021 tatcagggat ttataaaaag ggcaaagatt aaatgaaaga aataattatt ttacttggtg
    10081 ctcctggtgc tggtaaagga acagttggag aaagattgag taaaatttat aatattccag
    10141 ttgtttcaac aggtgaccta ttgagagaaa atgttaaggc aaaaacagaa cttggtctta
    10201 aagcaaaaga atatatggat aggggagaac ttgtacccga tgaaatcgtg atttcaattc
    10261 tggcagaaag aataaaaaaa aatgactgta aaaatggttt tatacttgat ggctttccaa
    10321 gaaatataaa tcaggcaaga aaacttgaag attatataaa agaggaaaaa gtaaaagttg
    10381 tatatctaaa agcagatgat gaatttcttg tgaagcgact ttccaataga agaatatgtg
    10441 aaacctgtgg agcaatatat catctaataa atataccgcc taaggttccg ggtgtatgtg
    10501 ataaatgcgg cggtaaattg attcagagag aagatgataa agaagaagtt gtaaggaata
    10561 gattagttgt ttatcataat ttgacagcac ctttacttga atattataaa aaaaagggaa
    10621 ttttatttac tgttcctgga gatggaaaac ttgaagaaat aatcaatcaa ataattaatc
    10681 ttaaaaataa tggagaaaaa ggaaattgaa atggtaagaa ttgcttgtaa aaaagcaaga
    10741 gagataatag aagaattgaa agaatattta gtagaaggga tttcaactta tgaaattgaa
    10801 cttaagagta ttgaaatgat gaagaaaaaa aatgttgaat ctgcttttaa ggggtataga
    10861 ggatatccag caaatatatg tatctcaatt aatcatgaag ttgtccatgg aatccccaaa
    10921 aagggtaaaa ttttaaaaaa tggagatata gtttcaattg atgttggagt aaaatataat
    10981 ggtttttatg gtgattgtgc agatactttt cctgttggca aagttgatgt aaaatatatt
    11041 aaattaattg aagttacaaa aaaagcattt ttaaagtcac ttaaatatgt ttatgctggt
    11101 tcaaagacag gcagtatagg ttctacaata gaagaatatg taaaaagaca tggattttct
    11161 gtaataagag attttaccgg tcatggaata ggaaaaaatt tacatgagtc acctgaaata
    11221 ccgaattttg gagagaaagg gaaaggagat attttactgg aaaacagaat tatagcaata
    11281 gaaccgatga tagcggaagg tacaggagaa atagaaatac ttgaagatgg atggacagca
    11341 gttacaaaag atagaggata tgctgctcat tatgaaaatt gtgtacttat taaaaaagat
    11401 ggatatgagg ttttaagtta aaaatggcta aagaagaaaa aattaaaatg gaaggagtaa
    11461 taaaaaaagt gttgccagca acaacttttg aagtagaact tgaaaatggt catgaaatta
    11521 ttgctcatct atgtggaaaa atgagaatga attatataaa aattgttcct ggagataaag
    11581 ttatagttga aatttctccg tatgatttaa aaagaggaag aattattaaa agaatataaa
    11641 aggagaaata aaatgaaagt tagagcatct gtaaggaaga tttgtccaaa gtgtaaaata
    11701 ataaagagga aagggattgt cagagtaatt tgtagtaatc ctcaggatgg accaaaacat
    11761 aaacaaagac agggataagg aggaacaaaa atggcaagaa tacttggagt tgaaatacct
    11821 gataataaaa aggtgaaaat tgccttgatg tatatttatg ggataggaga taaaagagca
    11881 gaagaaattg taaaaaatac aggagttaat ggagataaaa gagtaaaaga gttgaccgaa
    11941 gaagaacttg gtaaaataac ttcttttatt cagaaaaatt ataaaataga gggtgattta
    12001 aagcaggaaa ttgcacagaa tgtaaggaga ttgattgaga taaattgtta tagaggaata
    12061 agacataagt tgtctttacc tgttagagga cagagaactc gttcaaatgc aagaacgaga
    12121 aaaggaccca gaagaacagt tggtgttgtt agagataaag cagcaaggaa agcaattaaa
    12181 cagcaaaaag aggaaaaata aggaggtaaa aggtgccaag aaaatatata aaaaagagaa
    12241 aaaagaaaat aacagtacca aaaggtattg ctcatattaa agcaactttc aataacacta
    12301 ttattaccat tactgatcca gagggacatg ttttattatg gggaagtgcc ggtacaagtg
    12361 gttttaaggg tacaagaaag gggactcctt ttgcagcaca acttgcagca gaaaatatag
    12421 caaaaagagc ccagacaatg tttaatatga aggaagttga tgttaaagta aaaggacctg
    12481 gcccgggaag ggaaacagca attagagcat tacagagtgc tggattgaat gttgtttcta
    12541 ttaaagatgt aactccaatt ccccataatg gttgtagacc accaaagaga agaagagtat
    12601 agtgagaagg aggaaaaaat atggcaaaat atacaaaacc tttatgtaaa atatgtagaa
    12661 aatatggaat aaaactttat ttaaaaggta gaagatgtga aagtgataaa tgcgcagttg
    12721 accagagaaa gggtaaaata agattgataa aacagaaaaa aatgtctgaa tatggtcttc
    12781 aattgagaga aaaaaacaag gttaaaatat tttatggagt aatggaaaga cagtttaaaa
    12841 ggtattttga aattgctaaa aaacataaag gagttacagg agaagtattg cttcaattac
    12901 ttgaaagacg gcttgataat gttgttttta aagcaaactg ggttcattca agaaaaatgg
    12961 caaaacaatt aataaaccat gggcacataa ttataaacgg aagaagagtt gatagaccgg
    13021 gatatcttgt tgaaataggt gatataatac aggttaaacc aaatagtaag tatattaaaa
    13081 cagttaaaga atgtattgaa gaatataaaa caagaacaag gcctttatgg ttaaaagtag
    13141 atgatgagaa ttttaaaata gaaatattga gatatccgga tagaagtgaa gtttcaattc
    13201 ctgtagaaga acagttaata attaaccttt attcaaaata aaaaatagtg aggtgaaaaa
    13261 tgaaagaatt tatttatcca tcaaaatttt tatgggaaaa agagacactg agaaaaaatt
    13321 acggtaaatt gattatagaa ccacttgaaa gaggatatgg tgtaacaata ggaaatgctt
    13381 taaggagagt ccttctttct tccattccag gtgttgcaat tacagggtta agaataaaag
    13441 gtgtcttaca tgaattcgca acacttgaag ggatgaaaga ggatattgta gaggttgttt
    13501 taaatgttaa acagattgct ttaaaaccca taataaatga atatccccat acggtaagtg
    13561 tagaaatttc tgataaagaa gaaatctgtg caggagacct tgtaaatgat ggttcagtag
    13621 aggttattaa taaggattta cacattgcaa ctgttgatcc gagtaaaaaa tatcaatttg
    13681 aaattgatat tacaaaagga tttggatatt tacccattga aaaaatgaaa ttgatgatta
    13741 aagatttacc tgttggaacg attcttctgg atggacttta ttctcctgta agaaaggtta
    13801 ctttccatgt tgaaaataca agagttgaac agtttgttga ttatgaaaaa ctcattcttg
    13861 aagtatggac cacaggagca gtaactcctg aagagagtgt taattatgca tgtgaacttc
    13921 ttgaaaagca gtttgaactt attataaagg gacagaagat agaagaagag gaagaggaaa
    13981 ttacggaaaa gaaggaaata aaagatgaac ttgatattcc aattactgat ttaaaacttt
    14041 caacaagagt atataatgct cttgagaata aaaacataaa aacattgaga gaccttttaa
    14101 atacaccaat tgaaactctt gaagaaatga aaaatcttgg taaaaaatca cttgaagaaa
    14161 ttgaaaaggc attaaagaaa agagggtatc gtcttaaatc ttcctctgaa cttgctcaaa
    14221 caaaggagtc aaaatgaggc ataagaaaga tattaaaaaa ctcggtagaa ataaatcaca
    14281 cagaaaagca ctaatgagaa atcttacaat aagttttttt cagaaccaaa aaattgtgac
    14341 aactgtccct aaggcaaagc agttgagaag gattgttgaa agattaataa cacttgcaaa
    14401 aaagggaact ttagcggata taagaagaat aaatcaattt ttaaatcatc ctcaaacagt
    14461 aaaaaaaatt attgatgttt caaaagattt tctgatagaa atggtggata tacccagatt
    14521 ataaaaattg gatt
//
LOCUS       DAOSAT010000046        14376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2863_length_14376_cov_4.888066, whole genome shotgun sequence.
ACCESSION   DAOSAT010000046 DAOSAT010000000
VERSION     DAOSAT010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14376
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2863_length_14376_cov_4.888066"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PLF90_05220"
     CDS             complement(<1..761)
                     /locus_tag="PLF90_05220"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOK56712.1"
                     /translation="MPTKKEIISIIQNVGLWDRKSIEQALEEQERTGQPLREIFQGRG
                     MLPFGEIGPSFYFQLGIVQRELPAREISSEVVSILPPKIVKNHRIVPWDKRPDTLVFV
                     TDDPINILAADYFKKLANIEGVKNVEVIVTFKEEIDQLIDKYYGQDEMEDLTTMLQEA
                     SSATVDMDLPNLEEIDEDEETLALQAPVVKIVNLIFVEALRRRASDIHLEPLEKKFRV
                     RFRIDGVLYEIVSPPKRIERAVIARIKLMSKMDIA"
     gene            complement(796..996)
                     /locus_tag="PLF90_05225"
     CDS             complement(796..996)
                     /locus_tag="PLF90_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56713.1"
                     /translation="MKRLGPQTKKLFEVSRKEEKNKGKNKKYTFILSVDLMKKVKHKA
                     VEEEKTVSQFITEALESFLNKY"
     gene            complement(993..1763)
                     /locus_tag="PLF90_05230"
     CDS             complement(993..1763)
                     /locus_tag="PLF90_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK56714.1"
                     /translation="MNIIGFSNQKGGTGKTTICVNLAAGLVKLKERVLIIDLDPQGNS
                     TVSVGINLKPDDLTVREFILGEARIEDVMVDSYIADLSIIPSNSDLAHVEVEIANKSK
                     NQFYLRKAIENSKDVLKSFDFIFIDCPPSLSLLTVNGLCACKYVIIPVLADYLSLQGL
                     VNLLETIDMINKKLNPDLEILGIVPNMLDYRLKITEESLDLLREKFKDKLFKNGIKVC
                     SRLRESPSYGKAIFDYAKNSQGAIDFLNLAKEIKRRLK"
     gene            complement(1767..2948)
                     /locus_tag="PLF90_05235"
     CDS             complement(1767..2948)
                     /locus_tag="PLF90_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOK56715.1"
                     /translation="MEDRMKWWREAKFGMFIHWGIYSVPARGEWVMFCERIPKEKYKK
                     FARKFNPKNYNPDDWVLIAKDAGMKYMVLTTRHHDGFSLFDSKVSDFTAPKYINRDLI
                     AEYVESCRKYGLKIGFYYSLVDWRYDAFFLGPDKDPSGWQELVDYVHSQVKELMTNYG
                     KIDILWYDGAGPYKPEHWQSEKLNKMVRELQPHIIINDRSLLPEDFDTPEQHIKPSNR
                     DWESCMTLNDHWGYCKYDDNYKTPKQVIINLVRCASYGGNYLLNVGPRPDGRIPVKCI
                     KILKKVGEWLKVNGESVYGTKRTDFEWYHFHPGFITKRGNKLYLHIFYWFKEFAIGNL
                     KINVKNAYFLNEGISVDFKVEHDRVVFKNLPEKPPDPIDTVIVVEYEGEIEKLNSKRP
                     F"
     gene            complement(2958..5165)
                     /locus_tag="PLF90_05240"
     CDS             complement(2958..5165)
                     /locus_tag="PLF90_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012736152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOK56716.1"
                     /translation="MKVKEILKKMTIEEKVMQLCSVYITDLVKDGEISEDILKKELKL
                     GIGQISRVYGGIKNIEPEKAKEYLEKIQKFLREKTRLGIPAIIHEECLSGFLTNKATS
                     FPQIIGIASSFNPLLVVKMTEVIRRQMRNAGVHQGLSPVLDVCYDVRWGRTEETFGED
                     PYLCAIMGLHYVKGLQGEDLKEGIIATGKHFVSHGFSEGGRNIYPVHVGERELRDIFL
                     FPFEACVKEGNLKSIMNAYHDIDGIPCASSKKLLTDILRKEWGFDGIVVSDYGAVKML
                     NSTHFVAKDDKECAILSLKAGIDIELPNKGCYPLLIECVKKGLIKEKEIDEAVERVLR
                     VKKELGLFEENRKFYIDFDRDEDRKLAYEIAKETIVLLKNDGILPLKNIKSVSLIGPS
                     VENPRNYFGDYAYTAHLNLEKPSVECETILDVFKEKGIEIYYEKGCEIFDFNKDNFSK
                     AVEVAEKGDVIIFVGGDKSGFSSDCTCGEGKDSHNLKLPGVQEELILKLSEIGKPLIV
                     LLITGRPYILTEIIDKVNAIVECWFPGEETGNVIFDMLFGRFSPSGKLPVSFPKSVGQ
                     LPVYYHRKPVSKERKYVYSDSQALFPFGYGLSYSTFEYFDFEVKKKKIKAGDKFFVSV
                     SVKNTGNMEAAEVIQLYIRKKFSSVVLPSKLLKGFCKVNLKAGEEKTVIFEIPSEILA
                     FTDQNMKLKIEEGIYEVMIGSSSEDIKFKDEIEIRGNKFIKTRKLFFSKFSIK"
     gene            complement(5222..6667)
                     /locus_tag="PLF90_05245"
     CDS             complement(5222..6667)
                     /locus_tag="PLF90_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HOK56717.1"
                     /translation="MRKDIWRPIYHFMPPSGWMNDPNGLIQFKSKYHLFYQYNPYGVQ
                     PPYPPCSKTGAHWGHAISDDLLFWKDLPIALFPNKNKPDKDGCWSGSAVNNNGILTLI
                     YTGVRPECICVAESKDGVKFEKSPLNPVIKSPPENFEVTGFRDPYVWKEKDLWYMIVG
                     SGVKERGGIAFLYKSKNLLHWEYGGVFCEAGIEYGYMWECPNFFKVKDKYVLVVSSVP
                     LGYPIYFIGKYKNNKFKIEKQGKVDLFPFSFYAPQIFKDDKDRVLMFGWLRELTKKEF
                     HEEQGWAGVMSLPRILDVENNELKVNVIPEINKLRSSLLLDVSDIEITDEKTFCLKDM
                     EQNSVEIDALINLIDTDFFKMVLLFSDLTDENINIGYDKNEKTFFVDTVNSSLYETHH
                     KTLIKEKMDIENNEIFMQIFVDNSVIEIFLNNKFSFSLRVYPSKFTGLNLSFLVNNGK
                     LKIKRLSLWEMKNIWLISNSERINSINKSFE"
     gene            complement(6971..8221)
                     /locus_tag="PLF90_05250"
     CDS             complement(6971..8221)
                     /locus_tag="PLF90_05250"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK56718.1"
                     /translation="MLELIKKEIFFEKIKTIFRSLQYRNYRLFFIGQSISLIGTWVQR
                     IALPWLVYHLTHSTVLLGITGFAEQIPTFLLATIGGVVVDRKDKYRILLTTQILATIQ
                     ALILAILTLTGTIKVWEIIFLGIFLGCINAFDMPSRQAFVVDIIEKKEDLPNAIALNS
                     SMVNSARVVGPSLAGIIIGMTNEGICFLINAISYIFVVISLLLINIPSRNLKTKETNI
                     FQELKEGFLYVKNYSYLKYVILLLALVSLMGMPYTILMPVFVTKILKGTSKTFGFLMA
                     AGGFGALIGAFYLAGRKNTEGLEKIIKISAIVFGSGLIMFSISRFFSLALIFMVITGF
                     GMVLQIASSNTFLQSFVDDDKRGRVMSFYTLAFMGTAPFGSLLAGYLGKFLGVPLTLF
                     IGGFSCIIGALIFSYFLGKIEFKS"
     gene            complement(8321..9532)
                     /gene="dinB"
                     /locus_tag="PLF90_05255"
     CDS             complement(8321..9532)
                     /gene="dinB"
                     /locus_tag="PLF90_05255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOK56719.1"
                     /translation="MERVIFHIDMDAYFASVEQLTNPFLKGKPVAVSGGPSTKSVVAS
                     CSYEAKKYGVKSGMSTYQALFLCPHLIIVPGNSEKYIETSKKIFEIIRSFKEDIEIYS
                     IDEVFIDVSEIYELYGGKQYLAEEIKNKIKKQTGLKCSIGSGPNRLIAKICSSLSKPD
                     GIKICEENEVEDLMKNLPVSEIPGIGEKTYKKLSDMGIEKCGDIKKAGKNFFVRIFGK
                     LGERIYNYACGIEKPQIQIEIPEVSIGHSFTFSFDTDDIDTINKTLFNLCERVAERMR
                     KKKKHGNFISLFLRFEDFSSILKRKRFNDIPADGSTIFNLYCEIISSLQIEKKIRGIG
                     ISVGGLFNPECQYVFEEKIKREMVFYYVDRINEKFGENTLIPAILLNTSKIRKTHSFY
                     LWTLKEQKINI"
     gene            complement(9535..9780)
                     /locus_tag="PLF90_05260"
     CDS             complement(9535..9780)
                     /locus_tag="PLF90_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56720.1"
                     /translation="MITEINEKIIVGAIFKNGSIKINWFIWKNRKINIEKITYRWKTK
                     KGEKDIYNFAVTDGFNIFELSFIPEDLIWNLEKIHLL"
     gene            complement(9777..10367)
                     /gene="lexA"
                     /locus_tag="PLF90_05265"
     CDS             complement(9777..10367)
                     /gene="lexA"
                     /locus_tag="PLF90_05265"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOK56721.1"
                     /translation="MKNLTEKQRKIFEFINDYIIKNGYPPSIKEIMKHFSFSSPTAVV
                     SHLNALEKKGYIKREEKTSRGIILKKHFLNIPVIGRIPAGIPNIEEENLEGFISVNGQ
                     FLGKGEFFSLIVKGDSMKDAGIFDGDYVIIRKNAEVENGDIAVVFYNGEYTVKYFYKR
                     KNFIELKPANPSYKSIIIKENPLIIGKVVGLFRKIK"
     gene            complement(10420..11400)
                     /locus_tag="PLF90_05270"
     CDS             complement(10420..11400)
                     /locus_tag="PLF90_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOK56722.1"
                     /translation="MKEKKEEFLNTSFLYPEKKPTVVISRCLNFENTRYNGGIIKDEF
                     VLKLIRFVKYITVCPEVDIGLSVPREPINIFNYGEDFRLIQEKTNLDLTEKMNIFCEK
                     FLNSLNNIDGFLLKSKSPSCGVSGTVIYYEKDGKKSGYRGKGFFAMRVKEKFPYLPVE
                     DEARLKNPDIKFHFLVRIFSFFELNETLKNMISIKQLLDFHQNYKYLLMAYNQKILRF
                     LGNFLANYDNSKNIGEIKENYRVNFYKAFSKKMNPKSNINVIQHIYGHFKNRLNQKEK
                     RHFLDLLGRYKKNEIPLILLIEILKNFAYRFENEYLIKQKYLNPFPSDLF"
     gene            complement(11462..11920)
                     /locus_tag="PLF90_05275"
     CDS             complement(11462..11920)
                     /locus_tag="PLF90_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HOK56723.1"
                     /translation="MKRIAGILLIGLLLFSGCETIGNLSEKTKQGATIGAVAGGILGA
                     IIDSNKPWRGAIIGAATGAVAGGWIGNTLEKKSEEKVVEANKDVVTQAANEAGKLNAV
                     VKYSRVTENGVKEEIVATPGQLKDNKRMVTVEYLRDGKLISKEVREVTIN"
     gene            complement(11944..>12216)
                     /locus_tag="PLF90_05280"
     CDS             complement(11944..>12216)
                     /locus_tag="PLF90_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOK56724.1"
                     /translation="AESIKKLKNNAKIIKENNLKVLLVGSASPEGSSEYNLKLSEKRV
                     NAVRNYLIEKEGISENQLKTKADGEIEVPKKEWPAARKVSFSVISE"
     assembly_gap    12217..12316
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12317..12732)
                     /locus_tag="PLF90_05285"
     CDS             complement(<12317..12732)
                     /locus_tag="PLF90_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008986912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56725.1"
                     /translation="MKKVFLILVAVSFFLLVGKGFCQKASSEVSLPEEFSWYGQSGAQ
                     KEPVYDNAKKGYWWIPTKPPEGKENELWGNRGYIFVGSKKAEEKEEKEEKPVEKKVVR
                     EGCGKVVYVEKPVEKIVYVEKPVEKIVERVIEKPVE"
     gene            complement(12751..13152)
                     /gene="mscL"
                     /locus_tag="PLF90_05290"
     CDS             complement(12751..13152)
                     /gene="mscL"
                     /locus_tag="PLF90_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOK56726.1"
                     /translation="MKIIKEFKEFAIKGNMMDIAVGIIIGAAFGKIVSSLVNDILMPS
                     IGILLGGIDFSGLAFTLKGIGDRPPVTISYGKFIQTFVDFLIVAFALFFIVKGINKLK
                     KQQEIPPSPPIPSKEELLLTEIRDILKEKKI"
     gene            complement(13166..14176)
                     /locus_tag="PLF90_05295"
     CDS             complement(13166..14176)
                     /locus_tag="PLF90_05295"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOK56727.1"
                     /translation="MKKYFLIFLFSSFLLFAQEGYLRITSNPSGANIEVDGKTAGKTP
                     LLLALKPGDHTVKATLSGYNSITQTVKIIENEVIILQLTMEKQTQKTTTSFPFEKSKG
                     KLTIITDREDVTIYLNGKKVNAVPPVTLENVPTGSNSVILVSGDYADSYRVLILPGKT
                     FVLKHSFEETEKKSLSYQFRTSEDERKKLEEIEAKRSNLPAKIILKLSVPEVQKTTTG
                     QEVPIWGENDVVEVSFQYKKSEETNWNSKGLQSKTKQEESFEIPKGNYEIQLISAHYK
                     EPTGLINILLGTKKEKIKEYKEVMKKEIQPDTQYTFFITYDTKTGFSYKVEEKKLNTP
                     VE"
BASE COUNT         4572 a         2658 c         1629 g         5417 t
ORIGIN      
        1 tctgcaatat ccatttttga cataagtttt attcttgcaa taactgctct ttcaattctt
       61 tttggtggag aaacaatttc atataaaacc ccatctattc taaacctgac tctaaatttt
      121 ttttcaagtg gttcaagatg aatatcactt gctcttcttc tcaatgcctc aacaaaaatc
      181 aagttaacta tcttaacaac aggcgcctgt aaagcaagtg tttcttcatc ttcgtcaatt
      241 tcttcaaggt ttggtaaatc catatcaaca gttgcactac ttgcttcctg aagcattgtt
      301 gtcaaatctt ccatttcatc ctgtccataa tatttatcta ttaactggtc tatttcttct
      361 ttaaaggtta caataacttc aacatttttt acaccttcaa tgtttgcaag ttttttaaaa
      421 tagtcagcag caagaatgtt tatggggtca tctgttacaa aaacaagtgt atcaggtctt
      481 ttatcccatg gaacaatcct atgattttta acaatttttg gaggaagaat actaacaact
      541 tcagaagaaa tttctcttgc tggtaattct ctctgaacaa taccaagttg aaaataaaaa
      601 gaaggaccta tttctccaaa aggaagcata ccccttccct gaaaaatttc ccttaatggc
      661 tgacctgtcc tttcctgttc ttcaagggcc tgttctatac tttttctatc ccacagacct
      721 acattttgaa ttatacttat tatttctttt tttgttggca taccatttta tatattacct
      781 taaaaaacaa aaaagtcaat atttatttaa aaatgattcc aatgcttctg taataaactg
      841 agaaactgtt ttttcttctt ccactgcttt atgttttact tttttcatta aatccactga
      901 taatatgaat gtatattttt tatttttacc cttgtttttt tcctctttcc tgctcacctc
      961 aaacagtttt tttgtctgtg gtccgagtcg tttcatttca atctcctttt tatttcttta
     1021 gcaagattta aaaaatcaat tgctccctga gaattttttg cataatcaaa tattgctttg
     1081 ccataagaag gggactctct taatctactg catactttta taccattttt aaaaagtttg
     1141 tctttgaatt tttccctcaa taaatccaga ctttcttcgg ttattttcaa tctataatca
     1201 agcatattcg gaactattcc aagaatttca aggtctggat tcaatttttt atttatcatg
     1261 tcaattgttt caagtaaatt tacaagtccc tgcaaggaaa gataatcagc aagaactgga
     1321 ataattacat atttacatgc acaaaggcca ttcactgtca aaagtgaaag agagggtgga
     1381 caatcaataa aaataaagtc aaatgacttc aaaacatctt ttgaattttc tatcgctttt
     1441 cttaagtaga attgattttt acttttattt gctatttcaa cttctacatg agcaaggtct
     1501 gagtttgaag gaataattga taaatcagca atataactgt caaccatcac atcttcaatt
     1561 cttgcctctc caagtataaa ctctcttaca gtcaaatcgt ctggtttcaa gttaatacca
     1621 acactcacag ttgaattacc ttgcgggtct aaatcaatta ttaaaaccct ttccttcaat
     1681 ttaacaagac ctgctgcaag attgacacag atagttgttt tcccagtacc gcctttttga
     1741 tttgaaaaac ctattatatt cattttttaa aaaggccttt ttgaattaag tttctcaatt
     1801 tctccttcat attcaactac aattacagta tcaattgggt ctggtggttt ttccggaaga
     1861 tttttaaaaa caactctatc gtgttcaact ttaaaatcca cactgattcc ttcattcaaa
     1921 aaatatgcat ttttaacatt tatctttaag ttcccaattg caaattcttt aaaccagtaa
     1981 aaaatgtgga gatataattt attacctctt tttgtgataa atccaggatg aaagtgatac
     2041 cactcaaaat ctgttctttt tgttccatat acactttctc cattcacttt aagccattca
     2101 cctacctttt ttaaaatttt tatacacttt acaggaattc ttccatcagg tcttggtcca
     2161 acattcaaaa gatagtttcc accgtaactt gcgcatctta caagatttat tatcacttgt
     2221 tttggtgttt tataattatc atcatattta cagtatcccc agtggtcatt taaagtcata
     2281 catgattccc agtccctatt gctcggtttt atatgctgct caggcgtatc aaaatcctct
     2341 ggaagtaaac ttctatcatt tatgattata tgtggttgaa gttcacgtac cattttatta
     2401 agtttttctg attgccagtg ttcaggttta tatggtccgg ctccatcata ccacaaaata
     2461 tcaattttcc cgtaatttgt cataagttct ttaacctgac tgtggacata atcaacgagt
     2521 tcctgccagc cggatgggtc cttatcaggt ccaagaaaaa aagcatcata tctccaatca
     2581 acaagcgaat aataaaaacc aattttcaaa ccatattttc tacaactttc aacatattca
     2641 gcaataaggt ctctatttat atactttggt gctgtaaaat cactgacttt gctatcaaaa
     2701 agagaaaatc catcgtgatg tcttgttgtt aaaaccatat atttcatacc tgcatctttt
     2761 gcaattaata cccagtcatc tggattgtaa ttctttggat taaattttct ggcaaacttt
     2821 ttatatttct cctttggaat tctttcacag aacataaccc attcacctct tgcaggaaca
     2881 ctgtaaattc cccagtgaat aaacattcca aactttgctt ctctccacca tttcatcctg
     2941 tcttccattt tttctcctta ttttattgaa aattttgaga aaaataattt tcttgttttt
     3001 ataaacttat ttcctctgat ttctatttca tctttaaatt ttatgtcttc tgacgaactc
     3061 ccaatcataa cttcatatat tccttcttct attttcaatt tcatattctg gtctgtaaaa
     3121 gcaagtattt cagaaggaat ttcaaatatt actgtttttt cttcacctgc ttttaaattt
     3181 actttacaga aacctttaag aagttttgaa ggtagaacaa cagaagaaaa tttttttctt
     3241 atatataact gaataacttc tgctgcttcc atattccctg tatttttaac tgaaactgat
     3301 acaaaaaact tatcacctgc tttaattttt ttctttttta cttcaaaatc aaaatattca
     3361 aaagtagaat atgaaagtcc ataaccaaaa ggaaataatg cctgactatc tgaatataca
     3421 tatttccttt cttttgaaac cggctttctg tgatagtaaa caggcaattg tcccacactt
     3481 tttggaaagg atacaggcaa tttaccagaa ggactgaatc tcccgaataa catatcaaat
     3541 ataacatttc ctgtttcttc tcctggaaac cagcactcaa caatagcatt taccttatct
     3601 ataatttcag tgaggatata aggccttcca gttatcaaaa gaacaattaa tggtttccct
     3661 atttctgata attttaaaat taactcttcc tgaacacctg gcagtttaag gttatgactg
     3721 tctttacctt caccgcaagt gcaatcagaa gaaaaacctg atttatctcc accaacaaaa
     3781 attatgacat cacctttctc tgccacttca actgcttttg aaaaattatc tttattaaaa
     3841 tcaaaaatct cacaaccttt ttcataatat atttctatac ctttttcctt gaaaacatcc
     3901 agaattgttt cacattcaac agatggtttt tcaagattta agtgtgctgt ataggcataa
     3961 tcaccaaaat agtttcttgg attttcaact gaaggaccga ttaaagaaac agattttata
     4021 ttttttaatg gaagtattcc atcatttttc aaaaggacta ttgtttcctt tgcaatttca
     4081 taagcaagtt ttctgtcctc atctctgtca aaatcaatat aaaattttct attctcctca
     4141 aataatccaa gttctttttt tactctaaga actctttcta ctgcttcatc tatttctttt
     4201 tcttttatca atcctttttt cacacattct atcaaaagtg gataacaacc tttatttgga
     4261 agttctatat caatccctgc tttcaaagat aaaattgcac attctttatc atcttttgct
     4321 acaaaatgag tggaatttaa catctttact gctccataat ctgaaaccac aattccatca
     4381 aaaccccact cttttctcaa aatatcggta agtaattttt ttgaagatgc acagggaatt
     4441 ccatctatat catggtatgc attcataatt gattttaaat taccttcttt cacacatgcc
     4501 tcaaatggaa aaagaaaaat gtccctcaat tctctttcac caacatggac aggataaata
     4561 tttctaccgc cttctgaaaa tccatgggat acaaaatgtt tacctgttgc aattattcct
     4621 tcttttaaat cttctccctg taatccttta acataatgaa gtcccattat agcacataaa
     4681 taagggtctt ctccgaatgt ttcttctgtt cttccccatc ttacatcata acagacatcc
     4741 aaaacaggag aaagcccttg atgaacccct gcatttctca tctgtcttct tataacctct
     4801 gtcatcttca caacaagtaa aggattgaaa gaacttgcaa ttccaattat ttgagggaaa
     4861 gaagtcgcct tatttgtcaa aaaacctgat aaacattctt catgaattat agcaggaata
     4921 ccaagtcgtg tcttttctct taaaaatttc tgaattttct caagatattc ttttgctttt
     4981 tctggttcta tatttttaat accaccgtaa acccttgaaa tctgacctat ccctaatttc
     5041 aactcttttt ttaaaatatc ttcagaaatt tctccatctt ttacaaggtc tgttatataa
     5101 actgaacata actgcatgac tttttcttca attgtcattt ttttaagaat ttcttttact
     5161 ttcattttcc cctccatttc atctttttag tttataatag agagattttc gtgtcaattt
     5221 actattcaaa acttttattt attgaattaa ttctttctga attactgatt aaccagatat
     5281 ttttcatttc ccagagggat aatcttttaa tttttaattt tccattgtta acaagaaaac
     5341 ttaaatttaa acctgtaaat tttgaaggat aaactcgtag tgaaaaagaa aacttattat
     5401 ttagaaaaat ttcaattact gaattatcaa caaatatctg cataaaaatt tcattgtttt
     5461 caatgtccat tttttccttt atcaaagttt tatgatgggt ttcatataat gaagaattca
     5521 ctgtatcaac aaaaaatgtt ttttcgtttt tgtcatatcc aatatttata ttttcatctg
     5581 ttaaatctga aaataaaaga accattttaa aaaaatcagt atctattagg tttattaaag
     5641 catctatttc tacagaattt tgttccatat cttttaaaca aaaagttttt tcatctgtaa
     5701 tttcaatatc agaaacatca agtaaaagag aagaacgaag tttattaatt tcaggaatca
     5761 catttacctt taattcatta ttctctacat ccagaattct cggtaaggac ataactccag
     5821 cccatccctg ttcttcatga aattcttttt ttgtcaattc tcttaaccat ccaaacatta
     5881 aaactctatc tttatcatcc ttaaatatct gtggtgcata aaaagaaaaa ggaaataaat
     5941 caactttacc ttgtttttca attttaaatt tgttattttt atattttcca ataaaataaa
     6001 ttggataacc taatggtaca gaagaaacaa caagtacata tttatccttc actttgaaaa
     6061 aatttggaca ttcccacata tatccgtatt ctataccggc ctcacaaaaa actcctccat
     6121 attcccaatg taaaagattt tttgatttat ataaaaaagc aattccacct ctttctttaa
     6181 ctcctgaacc aacaatcata taccataaat ctttttcctt ccaaacataa gggtctctga
     6241 aaccagtaac ttcaaaattt tctggaggag atttaattac aggatttagt ggagattttt
     6301 caaactttac tccatcttta ctctctgcaa cacatataca ttcaggtcta actcctgtat
     6361 aaattaaagt aagaattcca ttattattaa ctgcactacc actccagcag ccatctttat
     6421 ccggtttatt tttatttgga aaaagagcaa tcggtaaatc cttccagaaa agtaaatcat
     6481 cacttattgc atgaccccag tgagcaccag tttttgaaca tggaggatag ggaggttgaa
     6541 ctccgtaagg attatactga taaaaaagat gatattttga tttaaattga attaaaccat
     6601 ttgggtcgtt catccatcca gaaggtggca taaaatgata aataggtctc catatatctt
     6661 ttctcatttt tttaaattat atactcaaat agtaaatatt tacacatcta cttatctatt
     6721 ttgttatatt tcgtttttct tggcaactac tctattttct taaaggggtt taaacccctt
     6781 taaaatcccc aagaggtatc aactccccca aacaccccct caatctttga gggggattta
     6841 tggtattttt gtcacttctg ggcaaaaaac gccagaaaaa acacatggaa ttttttaaat
     6901 tccagtgttt tatacccctg gttgagtaag tatttacctc aaatatcgga ttgaaaaatt
     6961 taaaataaaa ttaactttta aattctattt ttcctaagaa ataagaaaaa atcaaagcac
     7021 ctattataca ggaaaaacct ccaataaata aagtcagggg aacacctaaa aatttcccaa
     7081 gataacctgc aagtaaacta ccaaaaggtg ctgtccccat aaaagcaagt gtataaaaac
     7141 tcataactct tcctctttta tcatcatcca caaatgattg taaaaaagta ttacttgaag
     7201 ctatctgtaa aaccatacca aaacctgtaa taaccataaa aatcaaagca agagaaaaaa
     7261 aacgtgatat agaaaacatt ataagtcctg aaccaaaaac aattgcagaa attttaataa
     7321 ttttttcaag tccttcagta ttttttctac ccgcaagata aaaagcaccg attaaagcac
     7381 caaaaccacc tgctgccatt aaaaaaccaa atgtttttga agttccttta agaattttcg
     7441 taacaaaaac aggcatcaga attgtataag gcatccccat taaacttaca agagcaagta
     7501 acaaaattac atattttaag tatgaataat ttttaacata aagaaatcct tctttcagtt
     7561 cctgaaaaat atttgtttcc tttgttttta agtttcttga tggaatattt attaggagaa
     7621 gtgaaataac gacaaatata taacttatag catttataag aaaacaaatt ccttcatttg
     7681 tcattccaat tataatacct gctaatgaag gaccaacaac tcttgcactg ttaaccatag
     7741 aagaatttaa ggcaattgca ttcggtaagt cctctttttt ttctataata tcaaccacaa
     7801 atgcttgtct tgatggcata tcaaaagcat ttatacaacc aagaaatata ccaagaaata
     7861 taatctccca tacttttata gttcctgtta atgtaagtat tgccagtatt aatgcctgta
     7921 ttgttgcaag aatctgtgta gtcaacagaa ttctgtattt atcctttcta tccacaacta
     7981 cacctccaat agttgcgagt aaaaaagttg gtatctgttc agcaaaacca gtaattccaa
     8041 gtaaaactgt agaatgtgtt agatgataaa caagccatgg caaagcaatc ctctgaaccc
     8101 atgtacctat taaagagata ctctgtccta taaaaaaaag acgataattt ctatactgga
     8161 gagaacgaaa gattgtctta attttttcaa aaaaaatttc tttttttata agttctaaca
     8221 taacatacct ttcaaaagat ttttttataa agataaattt taactgtata ataaatcctg
     8281 tgtcaaaatt ttcaatgtgc tacttattaa aatatttctc ttagatattt attttttgct
     8341 cttttaatgt ccacagataa aaggagtgcg tctttcttat ttttgatgta tttaataaaa
     8401 ttgcagggat taaggtattt tcaccaaatt tttcatttat tctatctaca taataaaaaa
     8461 ccatttctct ttttattttt tcttcaaaga catactgaca ttcaggattg aataaacctc
     8521 caacagaaat tccaataccc cttatttttt tctcaatttg taaagaggat attatttcac
     8581 agtaaagatt aaaaattgta cttccatcag cgggaatgtc attaaatctt tttcttttca
     8641 aaatagatga aaaatcctca aatctcaaaa ataaagatat aaaatttcca tgtttttttt
     8701 tcttcctcat cctttctgcc actctttcac atagattgaa tagtgtttta tttatagtat
     8761 caatatcatc tgtatcaaaa gaaaaggtaa atgaatgacc tattgaaact tcgggaattt
     8821 caatctgtat ctggggtttt tctattccac aggcataatt atatattctt tcacccaact
     8881 taccaaaaat ccttacaaaa aaattcttac ccgctttttt aatatccccg catttttcta
     8941 ttcccatatc tgaaagtttt ttatatgttt tctctcctat acctggaatt tcacttacag
     9001 gcaaattttt catcaaatct tcaacctcat tttcttcaca tattttaatt ccatcaggtt
     9061 ttgatagaga tgagcatatt tttgcaatta atctatttgg tccagaacca attgaacatt
     9121 taagacctgt ttgttttttt attttatttt ttatttcttc tgcaagatat tgtttccccc
     9181 catataattc ataaatttca ctgacatcaa taaaaacctc atctattgaa taaatctcaa
     9241 tatcttcttt aaaacttctt ataatttcaa atattttctt tgatgtttct atatattttt
     9301 cagaatttcc aggaacaatt attaaatgcg gacatagaaa taaagcctga taagtactca
     9361 ttcctgattt cactccatat ttttttgctt cataagaaca ggatgcaaca actgattttg
     9421 ttgatggccc tcctgaaaca gcaactggtt ttccttttaa aaatggattt gtaagttgct
     9481 caacagaagc gaaatacgca tccatatcaa tgtgaaaaat taccctttcc atttttataa
     9541 aagatgtatt ttttcaagat tccagattaa atcttcaggg ataaaggata attcaaaaat
     9601 attaaaacca tctgtaacag caaaattata aatatctttt tctccctttt ttgttttcca
     9661 tctgtatgtt attttttcaa tatttatttt cctgtttttc catataaacc agtttatttt
     9721 aattgaacca tttttaaaaa tcgcaccaac tattattttt tcgtttattt ctgttatcat
     9781 tttatttttc tgaataaacc cacaacctta ccaataatca gaggattttc ttttattatt
     9841 attgacttat aagaaggatt tgctggtttt aactcaataa aattttttct tttataaaag
     9901 tatttgacag tatattcacc attgtaaaaa acaacagcaa tatctccatt ttcaacttca
     9961 gcattttttc taataataac ataatcacca tcaaaaatac ctgcatcttt catactatca
    10021 cctttaacaa tcagagaaaa aaattctcct ttacccaaaa attgaccatt aacacttata
    10081 aatccttcca gattttcttc ttcaatattc ggtattccag caggtattct tccaattaca
    10141 ggaatattta aaaagtgctt ttttaaaatt attcctcttg aagttttctc ctctctctta
    10201 atatatccct ttttttcaag agcattaaga tgtgagacaa cagcagttgg acttgaaaat
    10261 gaaaaatgtt tcataatttc ttttatagat ggaggataac cattttttat tatatagtca
    10321 tttatgaatt caaaaatttt tctctgtttt tctgttaagt ttttcatact gtaaataatt
    10381 atactgtaaa tttatataga tgtcaagagg aatttttagt taaaataaat cagaagggaa
    10441 tggatttaaa tatttctgtt ttataagata ctcattttca aacctgtaag caaaattttt
    10501 taaaatttct attaacaaaa tcaaaggtat ttcatttttc ttatatcttc ccagaaggtc
    10561 taaaaaatgt cttttttcct tctggttcaa cctgttttta aaatgtccgt aaatatgctg
    10621 aataacattt atatttgact ttggattcat tttttttgaa aatgctttat aaaaatttac
    10681 tctgtaattt tcttttatct ctccaatatt tttactatta tcataatttg caagaaaatt
    10741 cccaagaaat cttaaaattt tttggttgta tgccatcaaa agatatttat aattctggtg
    10801 aaaatcaaga agttgtttta tagaaatcat attttttaat gtctcattca attcaaaaaa
    10861 agaaaaaatt ctgacaagga aatgaaattt aatatcaggg tttttcaatc ttgcctcatc
    10921 ttcaacaggt aaatatggaa atttttcttt cactctcatt gcaaaaaatc ctttacctct
    10981 ataacctgat tttttaccat ctttttcata ataaattact gttccagaga caccacaact
    11041 tggagattta ctttttaaaa gaaatccatc tatgttattt aaagaattta aaaatttttc
    11101 acagaaaata ttcatttttt ctgttaaatc cagatttgtt ttctcctgaa tcaatctgaa
    11161 atcctcacca taattaaata tgtttatggg ttctcttgga acagaaagac cgatatctac
    11221 ttcaggacaa acagtaatat attttacaaa ccttataagt tttaaaacaa attcatcctt
    11281 aataatacca ccattatatc ttgtattttc aaaattaaga catcttgaaa taacaactgt
    11341 tggttttttt tctggataaa ggaaagatgt atttaaaaat tcttctttct tttccttcaa
    11401 tttccttttc cctaaatttt ttaattataa tacttctttc tataactttt aaaattaaga
    11461 atcaattaat cgtaacctct cttacttctt ttgaaatgag tttgccatct cttaaatatt
    11521 caacagtaac cattctttta ttatccttaa gttgcccggg tgttgcaact atttcttcct
    11581 ttactccatt ttcagttacc cttgaatatt tcacgactgc atttaatttt cctgcttcat
    11641 ttgctgcctg agtaacaaca tctttatttg cttctactac cttttcttct gattttttct
    11701 caagtgtatt tcctatccat ccaccagcaa ctgcacctgt tgcagcacca attattgctc
    11761 ctctccacgg cttgttagaa tcaattattg cacctaatat accacctgca actgctccga
    11821 ttgtagcacc ctgtttagtt ttttcagaga gattcccaat tgtctcacaa ccactgaata
    11881 acaaaagacc aattaataaa atccctgcaa ttcttttcat tttgacctcc tttaaaaaaa
    11941 ctttcactca cttattacag aaaaactaac ttttcttgca gcaggccatt cttttttagg
    12001 aacttcaatt tctccatctg cttttgtttt taattgattt tctgaaatcc cttctttttc
    12061 tattaaataa tttctgacag cattcactct tttttcagaa agtttaagat tatactctga
    12121 agacccttca ggtgatgctg aaccaacaag aagaaccttt aaattatttt ccttaataat
    12181 tttagcatta tttttgagtt tctttatact ttctgcnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnntttt caactggctt ttctattact ctttccacta ttttctcaac
    12361 cggcttttcc acatatacta ttttttcaac tggtttttct acatatacta cctttccaca
    12421 tccttctcta actactttct tctctacagg tttttcttct ttttcttctt tttcttctgc
    12481 ttttttactt ccaacaaata tataacctct atttccccat aattcatttt ctttaccttc
    12541 gggtggcttt gttggtatcc accagtatcc tttttttgca ttatcataaa caggttcttt
    12601 ttgtgcacct gattgtccat accaggaaaa ttcttcaggt aaagaaactt cagaagatgc
    12661 tttttgacag aaacctttac ctaccagaag aaaaaaacta acagccacca atattaaaaa
    12721 cacttttttc atactaatac ctcctttctt ttaaattttc ttttccttta atatatccct
    12781 tatttcagtt aaaagcaact cttcttttga aggaataggt ggagaaggtg gtatttcctg
    12841 ctgttttttg agtttattta tgccttttac aataaagaaa agagcaaaag ccactattaa
    12901 aaaatcaaca aatgtctgta taaactttcc ataacttatg gttacaggtg gtctatctcc
    12961 tatccccttt aaagtaaacg ctaaacctga aaaatcaatc cccccaagta aaatccctat
    13021 agaaggcatc aaaatatcat ttacaagaga agaaacaatt ttcccgaacg ccgctcctat
    13081 tattataccg actgcaatat ccatcatatt ccctttaata gcaaattctt taaactcttt
    13141 gattattttc atcatatctc ctttttcatt ctacaggagt attcagtttc ttttcttcta
    13201 ctttatatga aaagccagtt tttgtatcgt atgttataaa aaatgtgtat tgtgtatcag
    13261 gttgtatttc ttttttcatt acttccttat actccttaat tttttctttt ttagtaccaa
    13321 gaagtatatt aatcagccct gtgggttctt tataatgagc agaaatcaat tgaatttcat
    13381 aatttccttt gggtatttca aacgattctt cctgttttgt ttttgattga agtccctttg
    13441 aattccagtt tgtttcttct gattttttat actgaaaaga gacctctaca acatcatttt
    13501 caccccatat agggacttcc tgacctgtag ttgttttttg aacttcagga actgataatt
    13561 taagaataat tttagcagga agattactcc tttttgcttc tatttcttcc agtttttttc
    13621 tttcatcctc tgaggttctg aattgataag ataaactctt tttctctgtc tcttcaaaag
    13681 aatgttttaa aacaaaggtt tttccaggaa gaataagaac tctgtatgaa tcagcatagt
    13741 caccactaac cagtatcaca gaattacttc cagtgggcac attttcaaga gttacaggag
    13801 gaactgcatt aaccttctta ccgttaagat atattgttac atcttctcta tcagttatta
    13861 ttgtcaattt ccctttactt ttttcaaaag gaaaagaagt cgttgttttt tgtgtttgtt
    13921 tttccattgt gagttgtaaa ataataacct cattttcaat aatttttact gtctgtgtaa
    13981 ttgaattata acctgaaaga gttgctttga ctgtatggtc tccgggtttt aaagccaaaa
    14041 gtaaaggtgt cttccctgct gtttttccat caacttctat attggctccc gatggattgc
    14101 ttgttattct taaatatcct tcctgtgcaa aaagtaaaaa cgaagaaaac aaaaaaatca
    14161 aaaaatattt tttcattttt tccacctcct ttcttagaaa ttatttttta cttttagcat
    14221 tcacatgtaa gttttacctt ttattatatc acctgtcaag tttttttaat atttagttga
    14281 caagagtaaa ttattttttg tataatttaa aacaaaaggg cggttagctc agttggttag
    14341 agtgccacgt tgacatcgtg gaggtcacag gttcaa
//
LOCUS       DAOSAT010000047        14049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_2935_length_14049_cov_5.040875, whole genome shotgun sequence.
ACCESSION   DAOSAT010000047 DAOSAT010000000
VERSION     DAOSAT010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14049
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2935_length_14049_cov_5.040875"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1261)
                     /locus_tag="PLF90_05300"
     CDS             complement(59..1261)
                     /locus_tag="PLF90_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56728.1"
                     /translation="MKKGQVLLLVFLIVLIIGILGIGLLHMSGMESIQLRRQIHYLQA
                     LNIAEAGIERTLWKMASTPGWKDGYTEEVLENGFYTVLIEELPTPNRYKITSTGKVGY
                     GFGSILKTIYLEVEVRSKKWPFAFDYGLFWGNPSGSSSPLNLSNGVSVIGNVFVYGNI
                     NIGNNASVTNGSVYSTGTVTGGGTYQVGELPDPLPEKINFDTSSYDQLINIAYQKPKK
                     DFKLKNRQSYNLNGEILYVNGNVEVSNNCKIYGPGKIVATGDILIENNSEISGNVDLI
                     ANGTVTLRNNVVYQSDRTIIYGKTGITVWNNDNVGEKILLYTPGKLEVSQNVNVRGIL
                     WGGNVDLENNAYIKGAVYGDYFENNKIRNNVCIEYDPTIFEFDPPPGVPPGEGLVVKK
                     IHWSEDNF"
     gene            complement(1258..1746)
                     /locus_tag="PLF90_05305"
     CDS             complement(1258..1746)
                     /locus_tag="PLF90_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56729.1"
                     /translation="MEKTRKLKLFRSCRLTSNGFTLIELIIGIIIGIIIVLTVGYALV
                     YSNKNYKNQLIDYNITREGSFALFFMERKLRDKSPEKVEIQNDGDKIIIEENGNTTYI
                     TKSGGNLVYFDGSKESVLIKNIVEDINFEKEKDDLVKIYILLKQDNFSESMETKVKLR
                     NK"
     gene            complement(1694..2080)
                     /locus_tag="PLF90_05310"
     CDS             complement(1694..2080)
                     /locus_tag="PLF90_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOK56730.1"
                     /translation="MNKRGFTLIEGMIGILVFLIGILGGFAYFYFSKSTLELEIKRKI
                     ATEICHSRMEEIRTENYNNLLNFEENNTPVSVDKINGYRDTVIEDIDENGDGKTDYKK
                     ITVKVKWNQNGKDQEIKVVSFLSPYK"
     gene            complement(2151..2495)
                     /locus_tag="PLF90_05315"
     CDS             complement(2151..2495)
                     /locus_tag="PLF90_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56731.1"
                     /translation="MEKGDIILEVEPIVSSAEQSPFFAEAVDTNIRTAKTKVMVKDGE
                     TIVIGGLLRTEKTKRSTKVPVLGDIFPFLFKNRAETGKKTDLVMFLTPKIITEKEAKE
                     MVEKEKTRIEKK"
     gene            complement(2470..3432)
                     /locus_tag="PLF90_05320"
     CDS             complement(2470..3432)
                     /locus_tag="PLF90_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOK56732.1"
                     /translation="MRRKVFILFFIFIISVFGEEKLTLTFYQTKLAQVLQVLSKKTGI
                     KMITSSELSEKPISAYLENVSGEEAIDSILKANGLYREKLPDSDVYIIKEFKETPKLI
                     AEVIFLQYSKAEDLGKVLSPLLSKNGKIIVDTRTNSLTIQDTEENINDIKKIIKLLDK
                     SIPCIQIEAVLVELTEDGLKDLGIKWNIEANFFGPQEDVPYPYSMHIEREIVGPGGTT
                     TATGTTTGPQFILGRLSFQALTANLKLLEAKGEANILASPRVTTLNDSPATIKITKQM
                     AVAEKVVYTYGTAGTVAPTTKEPIFAEVGVTLVTTPHGKRRHYS"
     gene            complement(3429..3986)
                     /locus_tag="PLF90_05325"
     CDS             complement(3429..3986)
                     /locus_tag="PLF90_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56733.1"
                     /translation="MKNKNVLKLVILILILLIITGWQFKPKQKKMSKKENEGIFEEKE
                     MIGGIDLRKIEENFKSIQNKIMQMENKDKIELKYEKSKPLKNPLKSWLIKKESEKTVI
                     PEIQEEKPISIIKSEPKKPDFKISGIVYDKKISYVIINDEVKSEGEKVSDFVIQKIYP
                     EKIIVSDNEGNFFTLNFDFEKGERK"
     gene            complement(3990..4520)
                     /locus_tag="PLF90_05330"
     CDS             complement(3990..4520)
                     /locus_tag="PLF90_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56734.1"
                     /translation="MRDFYKILKRWFLWVIISYLFVIGIFTVIIFIPQKNQIVKYKTE
                     KDLLTYNYLKIKNDPEFFQTIKKTIDFAEDKIYNFEWLNYTDDPNLAIYEYLEEITPK
                     EFIEIISVKKEEIPSDLYFFWEVKLMGSFKGFFYFINRLESGRKYLKIEEIEVLEGEN
                     EKNIFNLKIAGIKKVK"
     gene            complement(4504..5112)
                     /locus_tag="PLF90_05335"
     CDS             complement(4504..5112)
                     /locus_tag="PLF90_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56735.1"
                     /translation="MIEINLLKRRLEKYRKKILLIRFIIFYLTGLVTIFLICGIFYLS
                     NKIIIERIKNEIKNLDKEIMAEKVIFENLKTNDKKLQILCQKCSFYEDEYKDRILWSD
                     IFWIISDSLPSGIWIKKLSYKKGYTQKGKNFNIFIEGYISPYYIQPEKGCSYFAKNLM
                     EKGKSIFEKISLLEIRKEKKEDNEIYYFKFELNLKEKNERFL"
     gene            complement(5109..6044)
                     /gene="pilM"
                     /locus_tag="PLF90_05340"
     CDS             complement(5109..6044)
                     /gene="pilM"
                     /locus_tag="PLF90_05340"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOK56736.1"
                     /translation="MSLKIGVDIGSKYIKIVEGYEKKDSLYLTNIEKIENPIENFRNI
                     SIEDTEILGNFLRDFLNKNNYKGKKCIFSIAGNNIIFHYFELPNLSEEELENAIKLEG
                     IQIIPDFLDNYEYDYISFNIDSKKNIFLISCPLIKVEIYNKIFLKAKLRPLIMDVDGL
                     AVLNSFLYFNKDTNSVCILNIGFSISNLVIFLKDKFLFCRDILWGTHNIEKIEEFEKN
                     VILNDIIPEFIEDINVSLKYFQNKTGEKINKMYLTGGGANISGIKEVIEKNINIPCEN
                     YNPLSFFSESLIPLDKIENGMSYAVCIGLLTRKII"
     gene            complement(6094..6495)
                     /locus_tag="PLF90_05345"
     CDS             complement(6094..6495)
                     /locus_tag="PLF90_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOK56737.1"
                     /translation="MKKNRKKGFTLIELMVVVIIVGILAAIAIPLYTGQVKKSKASEG
                     ASLVGSIRTAERVYYSQHNEYKVIEDAVSSDEDLGVDASGNKYFTTFSVDGTYAVAYG
                     SGDAADIQVRIKLDDGTLEWSTDGGESWKVF"
     gene            complement(6595..7812)
                     /locus_tag="PLF90_05350"
     CDS             complement(6595..7812)
                     /locus_tag="PLF90_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOK56738.1"
                     /translation="MPKYFYVARDFSGNRKEGVIEGETNATVISILRSRNLIPIEIKL
                     VDKNGISEMTIKPKKRGRVSLGEIAIFSRQLATMLDAGVPIIDAISDLKEQTINKYFA
                     YALENIRKDIQTGSNFSSAISKYPNIFSPLYVALVKSGEESGNLVGVMTELSSYIEDQ
                     IILLRKLRQALSYPAVIFLFFIGVISFVFLFLIPKFQSIFSSFGAKLPGLTLIVFKIS
                     GLFLKFLPFILIFLVLFGIFLFFYRNTKSGRYWIDSVKLKFPLIGKLVIKISLSRFSR
                     SLSTLLSGGVSINSALEIVAATSGNIIIEDIVKKIRKGVIEGKVLNEEMKKYKIFPPI
                     LVRMVRVGEETGRLEDMLNRVSKFFRDEVDATVNILTSIIEPILIIGLGIIVGIVVLA
                     IYLPIFKLAGTIR"
     gene            complement(7813..8883)
                     /locus_tag="PLF90_05355"
     CDS             complement(7813..8883)
                     /locus_tag="PLF90_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOK56739.1"
                     /translation="MSNFTIDDLLKYAVNQKASDIHLLAKLPPHIRIDGILYPVPFPA
                     LSNEECKKLIYSILSQEQIKIFENTKELDLSYGIDGVGRFRINLHYQRGTIGISIRPI
                     KDKIPTFEELGLPSDVIKKMLSNPYGLILICGPTGSGKSTTLASMVEYLNERESLHII
                     TIEDPIEYLFKYKKSIVEQREIGQDTYSFAEALKRVLRQDPDVVLIGEMRDKETIEAA
                     LRIAETGHLVLSTLHTGEVVQAMGRIVNVFSGDQQPTIRTQLSLVIIGVIVQQLIPRK
                     DGNGRILATEIMIATPAIKSLIREGNFAQIYSQIQLGGELGMKTMNASLISLYKKGLI
                     SKEEMYGRSNNIKELKLLLGEK"
     gene            complement(8880..10550)
                     /locus_tag="PLF90_05360"
     CDS             complement(8880..10550)
                     /locus_tag="PLF90_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOK56740.1"
                     /translation="MERKELGQLLLEKGLITSEQLEEAIRIQNETKKFLGEILVEKNY
                     VNKEDILNALTEQKKATYVDLSKFKGIKAEIVKLIDENIARRFTCIPIAKENDTLTIA
                     MKDPTDIIAIDTLKRITGMKIKVVKGDEFKIIELIDKFYSGFDNIPETISELEEITEE
                     KEEIDVAQLKVAAEDAPIVRFVNSLFVKAIEKRATDIHLEPGEKDVSVRFRIDGILHK
                     LPAPPKNAYPGVVTRLKILSNLDIGERRLPQDGRIKIKIGTKEFDIRISTLPTIYGEK
                     VVMRLLDKEKLVLNMEELGFEKEELEKFKNALKRPYGMIIVTGPTGSGKTTTLYSGLS
                     FINTPEKNIVTIEDPVEYQLEGINQVQIKPKIGLTFASVLRNLLRQDPDVIMVGEIRD
                     IETAQIAIQASLTGHLVISTLHTNDTVSTISRLYYMGVEPYLIADAINLVLAQRLVRK
                     ICNECKTIDEEGTEMLRNYYIERKDIIAYKGKGCSLCDFTGYYGRIAIYEVLEFTREI
                     REMVLKKKSDDEIRDFAKSHGMKILKEQAIKKVIDGITTLSEALTVTI"
     gene            10593..11474
                     /gene="speB"
                     /locus_tag="PLF90_05365"
     CDS             10593..11474
                     /gene="speB"
                     /locus_tag="PLF90_05365"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOK56741.1"
                     /translation="MEVIPFNFLSIPEISLKKAKFIVLPIGYEGTVTGNSGTKFGPIS
                     IIYSSRNLELYDEEISYQPYEVGVKTEEEFECDYSSPEKMVRKIEKRISYYVKNEKFV
                     IGLGGEHTITIGLFKGYKKYFSDLNYICLDAHSDLRDRYNGTKYSHACVSRRIYEENC
                     NLFILGVRSISKEESEFVNNKEKIKIYYACQMKKENWREKILREIPSGKYYLSIDVDF
                     FDPSLISETGTPEPGGFLWDETIDFLKNLILRNDIEIYGFDVVELSPSRILTPSSVIC
                     AKLIYKITGYLYLKWRK"
     gene            11462..12349
                     /locus_tag="PLF90_05370"
     CDS             11462..12349
                     /locus_tag="PLF90_05370"
                     /inference="COORDINATES: protein motif:HMM:NF027889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide 5'-hydroxyl-kinase"
                     /protein_id="HOK56742.1"
                     /translation="MEKINLLNEEQLNVFLSKEIKKILFIGGVDTGKTTLLKYISNFL
                     FKKGENVFILDCDVGQSHIGPPTTVGYAKLKEKIEDFNLDADRFYFVGSVTPSNSIIE
                     FIAGISIMNQFISKEKGKILIDTTGYVKNRLAISLKIHKIEILTPDFVFLLEKEKELE
                     EIERFLKFSGIKFTKIKVENIPVKSMEERNIFRKNRFLQYFGNLKKINLNMDKVSIKI
                     TNLRGVYNVFEIKNLNLRGNLCGLKRSNLDYFCLGVIANMKNEWAEIFIPEKVNINNT
                     DIKSISISNYSILEIVSVE"
     gene            12407..13798
                     /locus_tag="PLF90_05375"
     CDS             12407..13798
                     /locus_tag="PLF90_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOK56743.1"
                     /translation="MNNINKINKRNLNNLILKIQKEISKNKFINILSNLLTEGISINY
                     KGRIVYCNDKYAEIFGYKKEELIGENILKLCSTESKGEWEEILKRKKEGFFKTECIKK
                     DGSKIFINVNIKNVELGRKILRVVTVIDVTEEQLYLQKIKESEEKYRILTETLLDGIL
                     VIDIKGNFLFANHIAINLLGFKSFEELRKRNFFDFIIEKEKFLNDLRMIEENKSGYFT
                     EYEIIESTGSKLFIESIGRKINYSGIDAFLISFRDITERKVIIENLKRAIEKNKKILD
                     QTVIALSEMLSQRDPYTSSHQKRVAKLAISIAKEIGFTGSLIEGMKIAGLLHDIGKIY
                     IPADILSKPGELTDIEWQFIKLHPEFGAKIIKNIEFPWNIEKIVLQHHERINGSGYPN
                     KIGGKEILIETKILSVADVVEAMTFHRPYREKKTIKEALEEIETNSGILFEPEVVNAT
                     LKVFNKGFDFEDC"
BASE COUNT         4683 a         2171 c         1772 g         5423 t
ORIGIN      
        1 tggtcaatgt gtgctccaag aggacagatt ctataaggaa caaatattga atacattttt
       61 aaaaattatc ttctgaccag tgtatctttt taactaccaa tccttctcct ggtggaactc
      121 ctggaggagg gtcaaattca aatattgttg ggtcatattc tatacaaaca ttattcctta
      181 ttttattatt ttcaaaataa tctccataga cagcgccttt tatatatgca ttattttcaa
      241 ggtcaacatt tccaccccat aaaatacccc ttacattaac attttgagaa acttcaagtt
      301 tacccggtgt atataaaagt attttctctc caacattatc attattccac actgtaatac
      361 ctgttttacc ataaattata gttctatcag attgataaac aacattattt ctcaaagtaa
      421 ctgtaccatt tgctattaag tccacattac ccgatatttc tgaattattt tcaattaaaa
      481 tatcacctgt agcaacaatt ttcccgggtc catatatttt acagttatta ctaacttcta
      541 catttccgtt tacataaagt atctctccgt tcaaattata actctgccga ttttttaact
      601 taaaatcttt ttttggtttt tgatatgcta tatttatcag ttggtcatat gaagatgtat
      661 caaaatttat tttttcagga agaggatctg gtaattctcc tacctgatat gttcctcctc
      721 ctgtaactgt accagtagaa taaacagaac catttgtaac agatgcatta ttacctatat
      781 ttatatttcc ataaacaaaa acattcccta taacactaac tccattgctg agattaagag
      841 ggcttgaaga accagaagga tttccccaga aaagaccata gtcaaaagca aaaggccatt
      901 ttttacttct tacttcaact tcaagataaa ttgtttttaa aatacttccg aaaccatatc
      961 caacctttcc tgttgaagtt attttgtatc tattaggcgt tggtaattct tctatcaaaa
     1021 cagtatagaa tccattttca agaacttctt ctgtgtatcc atctttccag ccaggtgtac
     1081 ttgccatttt ccagagagtt ctttcaattc ctgcttctgc aatatttaat gcctgtaaat
     1141 aatgtatctg tcttcttaac tgtatagatt ccataccact catatgaagt agtcctattc
     1201 ccagaatacc tataattaaa actataagaa aaactaaaag caaaacctgt ccttttttca
     1261 tttgttcctc aattttactt ttgtttccat actttcagag aaattgtcct gtttcaaaag
     1321 tatatatatt tttaccaaat catctttttc cttttcaaaa tttatatctt ctactatatt
     1381 tttaattaaa acactttcct ttgaaccatc aaaatataca agatttccac ctgattttgt
     1441 tatataagtt gtatttccat tttcttctat tataatttta tcaccatcat tctgtatttc
     1501 aactttttcc ggtgatttat ccctcagttt tctttccata aaaaaaagag caaaagaacc
     1561 ttcccttgta atattataat caatcagttg atttttataa tttttattgc tgtaaactaa
     1621 agcatatcca acagtcaaaa caattatgat acctataatt attccaatta taagttcaat
     1681 taaagtaaat ccattacttg taaggcgaca agaacgaaac aactttaatt tcctggtctt
     1741 ttccattttg attccatttt accttaactg ttattttttt gtaatctgtt tttccatctc
     1801 cattttcatc aatatcttct attaccgtgt ctctatatcc atttattttg tcaacactaa
     1861 ccggtgtatt attttcttca aaatttaaaa gattattata attttctgtt ctaatttctt
     1921 ccatccttga atgacatatt tcagttgcta tctttctttt tatttcaagt tccagtgtgc
     1981 tttttgaaaa ataaaaataa gcaaatccac caagaattcc aattaaaaac actaaaattc
     2041 caatcatccc ttctattaaa gtaaatcctc ttttattcat ttttccccta atttattgag
     2101 aaaggcaaat aaaatataaa cgagtaattt ttctgttttg tttcttcttt ctattttttt
     2161 tcaattcttg ttttttcttt ttcaaccatt tcttttgcct ctttttctgt tattattttt
     2221 ggtgttagaa acatcacaag gtcagttttt ttacctgttt ctgccctgtt tttaaataaa
     2281 aatgggaaga tgtctcctaa aacaggtacc ttagtgcttc tttttgtttt ttcagttctt
     2341 aagagtcccc ctattacgat tgtttctcca tctttgacca taacttttgt ttttgctgtt
     2401 cttatatttg tgtcaaccgc ttctgcaaaa aatggagatt gttcagctga agagacaatt
     2461 ggttcaactt caagaataat gtctcctttt tccatgaggt gttgtaacaa gagtaacacc
     2521 aacttctgca aaaataggtt cttttgttgt tggagcaaca gttccagcag ttccataagt
     2581 ataaacaact ttttcagcaa ctgccatctg ctttgttatt ttgatagttg ctggtgaatc
     2641 atttaatgtt gttactcttg gactcgcaag tatattcgct tctccttttg cctcaagtaa
     2701 ttttaaattt gcggtaagtg cctgaaaaga taatcttcca agtataaatt gtggacctgt
     2761 tgttgttcca gtagcagtgg ttgttcctcc gggacctaca atttctcttt caatatgcat
     2821 actgtatgga tagggtacgt cttcctgtgg accgaaaaag tttgcttcaa tattccattt
     2881 tattccgagg tcttttaaac catcctctgt taattcaaca aggactgctt ctatctgtat
     2941 acatggaata cttttatcaa gtaattttat aatttttttt atatcattta tattttcctc
     3001 tgtatcctga attgttaaac tatttgttct tgtatcaact attatttttc catttttact
     3061 caataaagga gataaaacct ttcctaaatc ctctgctttt gaatattgaa gaaaaataac
     3121 ttctgcaata agtttcggtg tttctttaaa ttcttttatt atatatacat ctgaatcagg
     3181 taatttttcc ctatagagac catttgcttt taatattgaa tcaattgcct cttctccaga
     3241 tacattttca agatatgcac ttattggttt ttctgaaagt tcactgcttg ttatcatttt
     3301 tatacctgtt tttttactta aaacctgtag aacttgagca agttttgttt gatagaaagt
     3361 tagagttaat ttttcttctc caaaaacaga aattatgaaa ataaaaaata agataaaaac
     3421 ttttcttctc attttctttc tcctttttca aaatcaaagt ttaatgtaaa aaaatttcct
     3481 tcattatcac ttactattat tttttcagga taaatctttt gaataacaaa atcagaaact
     3541 ttttctccct cacttttaac ttcgtcattt attattacat atgaaatttt tttatcatat
     3601 acaatacctg aaattttaaa atctggtttt ttaggttcag atttgataat agaaatgggt
     3661 ttttcctctt gtatttctgg aataacagtt ttttctgatt cttttttaat tagccatgat
     3721 tttaaaggat tttttaatgg tttacttttt tcatatttta attcaatctt atctttattt
     3781 tccatttgca ttattttatt ctgaatactt ttaaaatttt cttctatttt tcttaaatca
     3841 ataccaccta tcatttcttt ttcttcaaaa attccttcat tttctttttt actcattttc
     3901 ttttgttttg gtttaaattg ccatcctgtt attattaaga gaattaatat caaaattacc
     3961 agttttaaaa catttttatt tttcatactt tattttacct tttttattcc agcaatttta
     4021 agattaaaaa tatttttttc attttcacct tctaaaactt ctatttcttc aatctttaaa
     4081 tattttcttc ctgattcaag acgattgata aaataaaaaa aacctttgaa actacccatc
     4141 aatttaactt cccagaaaaa atataaatca cttggaattt cttctttttt tacagaaata
     4201 atttctataa attcttttgg agtaatttct tcaagatatt cgtaaattgc aagattcggg
     4261 tcatctgtat aatttaacca ttcaaagtta tatattttat cttctgcaaa atcaattgtt
     4321 ttcttaattg tctgaaaaaa ttctggatca ttttttattt taagataatt atatgtaagt
     4381 aaatcctttt ctgtcttata tttaactatt tgattctttt gaggaatgaa aataataact
     4441 gtaaagattc ctattacaaa gagataactg ataattaccc acaaaaacca tctctttaaa
     4501 attttataaa aatctctcat ttttttcctt caagtttaat tcaaacttga aatagtaaat
     4561 ctcattgtct tctttttttt cttttcttat ttcaagaaga gagatttttt caaaaatgct
     4621 tttccctttt tccatcagat tttttgcaaa atatgaacat cctttttctg gttgaatgta
     4681 ataaggagaa atatatcctt ctatgaatat attaaaattt ttcccttttt gcgtatatcc
     4741 ttttttgtaa gaaagttttt taatccagat accagaagga agagaatctg atattatcca
     4801 gaatatatca ctccatagta ttctatcttt atattcatct tcataaaaag agcacttttg
     4861 acagagaatt tgtaattttt tatcattggt ttttaaattt tcaaaaataa ctttttctgc
     4921 cataatttct ttatcaagat ttttaatttc attctttatt ctttcaataa ttattttatt
     4981 tgatagataa aaaattccac agattaaaaa aattgtaaca agacctgtaa ggtaaaaaat
     5041 tataaatctt atgagtaaaa tcttttttct gtatttttct aatcttcttt taagtaaatt
     5101 aatctctatc atattatttt tcttgttaat agtcctatac agacagcata ggacatacca
     5161 ttttctattt tatcaagagg aatgagggat tctgaaaaaa aagagagagg gttatagttt
     5221 tcacaaggaa tatttatatt cttttctatt acttctttaa ttccggatat atttgctcct
     5281 ccacctgtca aatacatttt attaattttt tctcctgttt tattctgaaa atattttaag
     5341 gaaacattta tatcttcaat aaattcagga attatatcat taagaataac atttttttca
     5401 aattcctcta ttttttcaat attatgtgta ccccataaaa tgtctctgca gaataaaaac
     5461 ttatccttta aaaatataac taaatttgaa attgaaaatc caatatttag aatacaaaca
     5521 gaatttgtgt ctttattaaa atatagaaaa ctgtttaaaa ctgcgagacc atcaacatcc
     5581 attataagtg gtcttaattt tgctttcaaa aaaattttat tgtatatttc cactttgatt
     5641 aaagggcagg aaataagaaa aatgtttttt ttactatcta tattgaatga aatataatca
     5701 tattcataat tatctaaaaa atcaggtatt atttgaattc cttctagttt tattgcattt
     5761 tcaagttctt cttcagataa atttggaagt tcaaaataat ggaaaataat attatttcct
     5821 gcaatactga atatacactt ctttccttta taattgtttt tattgagaaa atctctcaaa
     5881 aaatttccta atatctcagt atcttcaata gaaatattcc tgaaattttc tataggattt
     5941 tcaatttttt ctatatttgt caaatataaa ctatcttttt tttcataacc ctctacaatt
     6001 tttatatatt tagaccctat atcaacacct attttaaggg acatattttt aaaaaaaaag
     6061 tggagttagg ttaactccac tttgcgtatc tttttaaaat actttccaac tttcaccacc
     6121 atctgtagac cattcaagag ttccatcatc gagttttatt cttacctgaa tatctgcagc
     6181 atctcctgaa ccataggcaa cagcatatgt cccatctact gaaaatgtag taaaatattt
     6241 atttccagag gcatcaactc caagatcttc atctgaactg actgcgtctt caattacttt
     6301 atattcattg tgctgtgaat aataaactct ttcagctgtt ctaattgacc caacaaggga
     6361 agcaccttca gatgcttttg attttttaac ctgtcctgtg tataaaggaa tagcaattgc
     6421 tgcaagaata ccaacaataa taactaccac catcaactca atcaacgtaa accctttttt
     6481 tctatttttt ttcatttttt tcacctcctt tctttaattt ttgacgttat tataaacttt
     6541 ttttctcttt ctgtcaaaaa tttttttatg atatcagagt tactatatat cccatcatct
     6601 aatagttcct gcaagtttaa atatgggtaa ataaatagca agaacaacaa taccaacgat
     6661 aatcccaaga cctattatta aaattggctc aattatagaa gttaaaatat ttacagttgc
     6721 atcaacttca tctctgaaaa attttgaaac cctgtttaac atatcttcta atctacctgt
     6781 ttcttctcca acccttacca ttctcacaag tattggcgga aaaattttat attttttcat
     6841 ttcttcattt agaactttac cttctataac tccttttctt atttttttaa caatatcctc
     6901 aattattata ttccctgaag tagcagcaac tatttcaaga gcagaattta tacttactcc
     6961 tcctgaaaga agtgtggaaa gagaacggga aaatcttgaa agagaaattt taatcacaag
     7021 tttaccaatt aaaggaaatt ttaatttcac tgaatctatc cagtatctac cagattttgt
     7081 atttctgtaa aaaaataaaa aaattccaaa taaaactaaa aatattaaaa taaagggtaa
     7141 aaattttaaa aataaaccac ttattttaaa aacaatcaat gttaatccgg ggagttttgc
     7201 tccaaaactt gaaaaaatag attgaaactt tggaattaga aataagaaaa caaaagaaat
     7261 aactcctata aaaaacaaaa aaattacagc aggataactt aacgcctgtc ttaattttct
     7321 taagagaatt atctggtctt ctatatatga tgaaagttct gtcataacac cgacaagatt
     7381 tccactttct tcccctgatt ttacaagtgc aacatataaa ggagaaaaga tattaggata
     7441 tttacttatt gcactggaaa aattacttcc tgtctgtata tcttttctaa tattttcaag
     7501 agcatatgca aaatatttat ttatagtttg ttcttttaag tcagaaatgg catcaattat
     7561 tggaacacct gcatcaagca ttgttgcaag ttgtcttgaa aatatggcaa tttctccaag
     7621 tgatactctt cctcttttct ttggctttat cgtcatttca gaaatcccat ttttatcaac
     7681 aagttttatt tcaattggta ttaaattcct gcttcttaaa atacttataa ctgttgcatt
     7741 tgtttctccc tctattactc cttcttttct atttccagaa aaatcccttg caacataaaa
     7801 atattttggc atttattttt ctcccaaaag taattttaat tcttttatat tattactcct
     7861 tccatacatt tcttcttttg aaatcaatcc ctttttatac agggaaatca aagaagcatt
     7921 catcgttttc attccaagtt ctcctccaag ttgtatctgt gaataaatct gggcaaaatt
     7981 tccctctctt atcaaacttt ttattgcagg tgttgctatc atgatttcag ttgcaagaat
     8041 tctaccattt ccatcctttc ttggtattaa ttgttgaact attactccta taattacaag
     8101 agaaagttga gtccttattg ttggttgctg gtccccagaa aaaacattta caatccttcc
     8161 cattgcctga acaacttctc ctgtatgtaa agttgataat acaagatgtc ctgtttctgc
     8221 tattcttaaa gccgcctcta ttgtttcttt atctcgcatc tctccaatta aaacaacatc
     8281 tgggtcctgt cttaaaaccc tttttaatgc ttctgcaaaa gaatatgtat cctgtcctat
     8341 ctctctctgt tcaacaattg attttttata tttaaaaagg tattcaatag ggtcttcaat
     8401 tgttataata tgtagtgatt ctctttcatt taagtattca accatggaag caagtgttgt
     8461 tgattttcca cttcctgttg gaccacatat taaaattaaa ccataaggat ttgaaagcat
     8521 tttttttata acatcagaag gaagtccaag ttcttcaaaa gtaggaattt tatctttaat
     8581 aggtcttatt gaaattccta ttgtccctct ttgataatgt aaattgattc tgaaccttcc
     8641 aacaccgtca attccataag aaagatcaag ttcttttgta ttttcaaata tttttatttg
     8701 ttcctgactc aaaattgaat aaattagttt tttacattct tcatttgata aagcaggaaa
     8761 gggtactgga tacaaaattc catctattct tatatgtggg ggcaattttg caagtaagtg
     8821 aatatcagat gctttctggt taactgcata ttttaataaa tcgtcaattg taaaattact
     8881 catattgtta ctgttaatgc ttcagaaagt gtggttatac catcaataac cttttttatt
     8941 gcctgttctt ttaatatctt cattccatga cttttagcaa aatctcttat ttcatcatca
     9001 ctttttttct ttaaaaccat ttctcttatt tctcttgtaa actctaaaac ctcatatatt
     9061 gcaatccttc cataataacc tgtaaaatca cagagagaac aacctttacc tttatatgca
     9121 attatatcct tcctttctat ataataattt ctcaacattt ctgttccctc ttcgtcaata
     9181 gttttacatt cattacaaat ttttcttacc aatctctgtg caagaactaa atttatagca
     9241 tctgcaatca aatacggttc aacgcccata taataaagtc gggaaattgt tgaaacagta
     9301 tcatttgtat gtagtgttga tataacgaga tgccctgtta atgacgcctg tattgctatc
     9361 tgtgctgttt caatgtctct tatttctcca accattataa catcagggtc ctgccttaat
     9421 aaatttctta aaacacttgc aaaagtcagt cctatttttg gtttaatttg aacttgattt
     9481 attccttcaa gttgatattc aacagggtct tcaatggtaa ctatattttt ttcaggtgta
     9541 tttataaaac tgagtcctga atataaagtt gttgtttttc cacttcctgt tggtcctgta
     9601 acaattatca ttccataagg tctttttaga gcatttttga acttttcaag ttcttctttt
     9661 tcaaatccaa gttcttccat atttaaaaca agtttttctt tatcaagtaa tctcataact
     9721 actttttctc catatattgt tggaagtgtt gaaattctta tgtcaaattc ttttgtccca
     9781 atttttattt ttattcttcc atcctgtggt aatcttcttt ctccaatatc aagattagaa
     9841 agaattttaa gtcgggtaac aactccagga taagcatttt tcggaggggc aggaagttta
     9901 tgtagtatac catctattct aaatctaaca gaaacatctt tttctcctgg ttcaagatga
     9961 atatctgttg ctcttttttc aattgctttt acaaaaagag aatttacaaa tcttactata
    10021 ggagcatctt cagccgcaac ttttagttgt gctacgtcaa tttcttcctt ttcctctgtt
    10081 atttcttcaa gttcagaaat tgtttctgga atattatcaa agccagaata aaacttatca
    10141 attaattcaa ttattttaaa ttcatcgcct ttaacaactt ttattttcat tcctgttatt
    10201 ctttttaaag tatcaatagc aataatatct gttgggtctt tcattgcaat cgttaaggtg
    10261 tcattttctt tcgctattgg aatgcaggta aatcttcttg caatgttttc atctattaat
    10321 ttaacaattt ctgcttttat ccctttgaat tttgagagat caacgtaagt ggcttttttt
    10381 tgttccgtta aagcgttcaa aatatcttct ttgtttacat agtttttttc aactaaaatc
    10441 tctccaagaa atttttttgt ctcattctga atcctaatag cttcttctaa ctgttctgaa
    10501 gtaattagcc ctttttcaag taagagttga cctaattctt ttctttccat aatttatagt
    10561 ataatatacc ataaaaaaag aaaggagtaa aaatggaagt aatacctttt aattttttat
    10621 ctattccaga gataagttta aaaaaagcaa aatttattgt tttaccaatt ggttatgaag
    10681 gaactgtaac aggaaattca ggaacaaaat ttggacccat cagtataatt tactcatcaa
    10741 gaaatcttga actttacgat gaagaaataa gttatcagcc atatgaagtt ggagtaaaaa
    10801 ctgaagaaga gtttgaatgt gattattctt ctcctgaaaa aatggtcaga aaaattgaaa
    10861 aaaggattag ttattatgta aaaaatgaaa aatttgttat aggattgggt ggtgagcata
    10921 caataacaat tggtttgttt aaaggatata aaaaatattt tagtgattta aattacattt
    10981 gtcttgatgc acattctgat ttgagagata ggtataatgg aacgaagtat tctcatgcat
    11041 gtgtaagtag aagaatctat gaagaaaatt gtaatttgtt tattttaggt gtaagaagta
    11101 taagtaagga agaatctgaa tttgtaaata ataaagaaaa aataaagatt tattatgcat
    11161 gtcagatgaa aaaagaaaac tggcgggaaa aaattttaag agaaattcca tctggtaaat
    11221 attatctctc aattgatgtt gatttttttg acccttcttt aatttcagaa acaggaactc
    11281 ccgaaccagg tggatttcta tgggatgaaa caattgattt tttgaaaaat ttaattttaa
    11341 gaaatgatat tgaaatctat ggttttgatg tggttgaact ttctccatca agaattttaa
    11401 ctccttcttc ggtaatctgt gctaaattaa tttataaaat aactggatat ttatacctta
    11461 aatggagaaa ataaaccttt tgaatgaaga gcaattaaat gtttttcttt caaaagaaat
    11521 aaaaaaaatt ctttttatag gtggagtgga tacaggaaaa acaactttat tgaaatatat
    11581 ctcaaatttt ttatttaaaa aaggagaaaa tgtttttatt cttgattgtg atgttggaca
    11641 atcacatatt ggtcctccca caacagttgg ttatgctaaa ttaaaagaaa aaattgagga
    11701 ttttaattta gatgcggata gattttactt tgttggttct gttacaccat ctaattctat
    11761 cattgagttc attgcaggaa tttcgataat gaaccaattt attagtaaag agaaagggaa
    11821 gattttgatt gatacaacag gatatgtaaa aaataggtta gcgatttctt tgaaaattca
    11881 taaaatagaa attttaaccc ctgattttgt ttttttactt gaaaaggaaa aagaacttga
    11941 agagatagaa aggtttttga aattttcagg aataaaattt acaaaaatta aagttgagaa
    12001 tatccctgta aaatcaatgg aagaaagaaa tatctttagg aagaatagat ttttacagta
    12061 ttttggaaat ttaaaaaaaa ttaacttaaa tatggataag gtttcaatta aaatcactaa
    12121 tttgagagga gtttataatg tttttgagat taaaaattta aatttaagag gaaatttatg
    12181 tggtttaaaa agaagtaatt tagattattt ctgtctcggt gttattgcta atatgaaaaa
    12241 tgaatgggca gaaattttta taccagagaa agttaatata aataacacag atattaaaag
    12301 tatttctata agcaattatt ctattttgga aatagtttca gttgaataaa gtaaaacttt
    12361 caaaaaaatt tgacaataaa aaatttttaa ttttataatt aacataatga acaatattaa
    12421 caaaattaac aaaaggaact tgaataattt gattttaaaa attcagaagg aaatttcaaa
    12481 aaataagttt ataaatattt tgtcaaatct tttaacagag ggaatttcta taaattataa
    12541 aggacggata gtttactgta atgataaata tgcagaaatt tttggatata aaaaggaaga
    12601 gttaatcgga gagaatatat taaaactctg ctcaacggaa agtaaaggag aatgggaaga
    12661 gattttaaaa agaaaaaaag aaggattttt taaaactgaa tgtataaaaa aagatggctc
    12721 taaaattttc ataaatgtga atattaaaaa tgtagaatta ggtagaaaaa ttttaagagt
    12781 tgttactgta attgatgtca cagaggaaca gttatatctt cagaaaatta aagaatcaga
    12841 agaaaaatat agaattctta ctgaaacact tcttgacgga attctggtga ttgatattaa
    12901 aggtaatttt ctttttgcaa atcatattgc aataaatctt ttaggattta aaagttttga
    12961 ggaattgcga aaaaggaatt tttttgattt tataattgaa aaagagaaat ttttaaatga
    13021 tttaagaatg attgaagaaa ataaatctgg ttatttcact gaatatgaaa ttatagaaag
    13081 tacaggttct aaattgttta ttgaaagtat tggaagaaaa ataaattata gtggtattga
    13141 tgcgttttta atatctttca gagatattac agaaagaaaa gttataattg aaaatcttaa
    13201 aagagcaatt gaaaaaaata aaaaaatttt agaccagaca gtaatagcat tatctgaaat
    13261 gttatctcaa agagaccctt atacttccag tcatcaaaaa agggttgcaa aacttgcgat
    13321 atcaattgca aaggaaattg gatttacagg tagtttaata gaaggtatga aaatagcggg
    13381 tttacttcat gacataggta aaatttatat tccagcagat attctttcaa aaccagggga
    13441 attgactgat atagaatggc aatttataaa actgcatcct gaatttggtg caaaaataat
    13501 taaaaatatt gaatttcctt ggaatataga aaaaattgtt ttacagcatc acgaaagaat
    13561 aaatggttca ggttatccaa ataaaatagg tggaaaagaa atattaattg aaacaaaaat
    13621 tttaagtgtt gcagatgttg ttgaagcaat gacatttcac agaccttaca gagaaaagaa
    13681 aaccataaaa gaagcacttg aagaaataga aacaaacagt ggaattcttt ttgaacctga
    13741 agtagttaat gcgactttga aagtattcaa taagggtttt gattttgaag attgttagga
    13801 tttttatagg agaagcatag gggacaattc tttcccctaa aaccccttta acctcgtaga
    13861 ttatttatta ctgttttctt atagagattt attttcctat gtgaatgccg aagaggggat
    13921 ttgaacccct acgaggttgt atctcaggag ggggtgcttc ccccttcctg aaatagttct
    13981 ccccccacga cgaaaagcat aggggacaat tctttcccct aaaacccctt taacctcgta
    14041 gattattta
//
LOCUS       DAOSAT010000048        13729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3021_length_13729_cov_5.577958, whole genome shotgun sequence.
ACCESSION   DAOSAT010000048 DAOSAT010000000
VERSION     DAOSAT010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13729
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3021_length_13729_cov_5.577958"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLF90_05380"
     CDS             <1..836
                     /locus_tag="PLF90_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK56744.1"
                     /translation="LRNYDWLDPISFYEKSLKWGFEDGKLYYNLGTIYYRMKNYEKAL
                     ELFSKSIDLFKNKKLPYLGIASCYANIGKYDEAINYYEKVLEIDPDNPFALCSIATIY
                     YNQRKKSLTEIFELLDKCIKKNPTYRPSYVLAGRIFYENDNFKESIKFFKIAVSIDPN
                     DDLSHLLLGISYFNTGNKEEAEKHLTKAYNLNPNNYENIQNLAFFYKITGKFDKALIL
                     YEEAIKLKPDDLDTLNDIGLCYAMIGEKEKAKNIWEEILKKNPDYKPAKTNLQILKKN
                     N"
     gene            complement(833..3124)
                     /gene="hypF"
                     /locus_tag="PLF90_05385"
     CDS             complement(833..3124)
                     /gene="hypF"
                     /locus_tag="PLF90_05385"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOK56745.1"
                     /translation="MKLEERYRILVKGKVQGVGFRPTIYRYAKNLGLKGYVCNTGSGV
                     LIEIEGEKNKIEYFIERIRKSPPSKAEIKEIDIRKIDIKNEKDFFILKSKKEETREVE
                     ISPDIATCEKCLRELFNQKNRRYLFPFINCTDCGPRFTIVKKVPYDRKNTTMDEFIMC
                     DTCQKEYKDVYSRRFHAQPNCCFTCGPTLKLFDNKGKEVAENVDAIKKGSELIKMGKI
                     LGIKGIGGYHLACDAENEKAVKFLRERKKRYGKPFALMARNLKVIEKFCYINNFEKKL
                     LKSWQTPIVLLKKKNEILPYEIAPENNYLGFFLPYTPVHHLLFHFGKNLNVLVMTSGN
                     FSEEPIIYEDEIAFKRLSGICDFFLTHNRKIYINCDDSVLRIFNKKIYFIRRSRGFVP
                     SPVEIPYTYNKIIFSAGSDMKNTFSFTKSKNVYLSQHIGDLETELSIESYKKSINLFK
                     NILEVEPEIIVCDMHPGYFSSKIAEEIFPDREKIYVQHHHSHITSCMADNFLKNEKVI
                     GVAFDGTGYGLDGKIWGGEFLICDYENFERIGHLRYFPLPGEISIKEVWRIGTVYLYM
                     VFGEDFLNLDIDFVKKIDMKKWGIIKKMVDNNINTFLNSSFGRFFDAVSSILSIREKI
                     DYEGQAAIELEMKIEKNKNLKPYEYKINKEKTGYIIEPEMILKGITDDLKKGLKTGEI
                     SYRFHITVSEIIKDMCKILREERDINKVALSGGVFQNMFLLNDTLKKLKKEKFDVLIH
                     NKVPVNDGGISLGQAVIGIFKKN"
     gene            complement(3121..3561)
                     /locus_tag="PLF90_05390"
     CDS             complement(3121..3561)
                     /locus_tag="PLF90_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOK56746.1"
                     /translation="MRKILIVYYTLGGNTEKAAEIIKKGIESEGLEVVLKKGIDADID
                     DLLSCDGIVIGSPDYFSYMAGGLKDFFDRTFYPSRGKVEGKPYFAFLTHGGGGKAIES
                     IEKIAASFKFKKVLDPILIKNAPNKEDEEKLIEAGKKFARIIKG"
     gene            3849..5180
                     /locus_tag="PLF90_05395"
     CDS             3849..5180
                     /locus_tag="PLF90_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOK56747.1"
                     /translation="MRKFFPKRFSLFIATLITLIEILLIFYFVQITENVYEMKFDLGG
                     ILFIYDDLGIIFSITSCFIAFLIFLYSFGYFKEEKERDYFSFWGLIFLGSMLGVIFSD
                     NLISLYFFWEIAGLCSYRLIGFYREKEHLLKADKAFMITVAGAGIMFLSFAYIYSITG
                     TFSLSEMKGISVSPFVFLLFFIGVITKSATFPFHTWLPDASVAPTPVTAFLHAAVLVK
                     IGIYGLLRIFWKTLTVEGNIVWALYLCIFSSLIAGIVALIENDFKKILAYSTVSQLGF
                     LITGILTFNNTGISGSLIFYIAHALGKGGLFLTAGLMEKVFKTKDITKMGGVMKNMPF
                     LTFGYLFSMFSVIGIPPFAGFFGKINIIMGVLKNHLLFAGFFLIISSILTLLYLWRTF
                     KYVFIFENATSNFDFNYKGSKIMTFSILTLGLFSFLIGIMLFFVKGGIFGY"
     gene            5170..7011
                     /locus_tag="PLF90_05400"
     CDS             5170..7011
                     /locus_tag="PLF90_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOK56748.1"
                     /translation="MDIKLLFLPVFVSFGFSFLNLLFYGIGRNIRKYLSIFGSLLVLI
                     SSIYILKYSVALHSSTLPNWENLSIFFAILISAISLFCIIYSQWYNPSANFVYDSLFF
                     LFTGSMLGVVLSGSLIYFYVFWELMTISSFFLVLYENTEESKNASIKYIVMTGAGSIF
                     LLFGIIGVNFIEKGIIWKHLFFFSIFIGAGVKSGLFPLYSWVPETYPSSPTPISAILS
                     GLASKMGIYAFIKFYFTIFQPGWSIAWENLMIITGMITLLSGVLLALIQHDIKRLLAY
                     HSISQIGYIFLGIACGTSIGLCGGLYHLLNHSIFKSLLFLGAGILIKMTHSRELEKYG
                     RFSSILPFTFYTFSIASLSISGIPPFNGFVSKWIICQALLEKKTGISIFAMIISLLGS
                     ALTLASFIKVLNDAFLGVGKGEIKEKINERTPLIIIPFGFLALGCIVFGISPSLVINK
                     IFSPIIGSIPTLNIKLINFISFWGYLSILVLFAGYIYLSRQTRKFKEKKVFVGGENIP
                     KEITDFDGSHFYKTVYETNGIKDFYEIERKGLFDSYRISEKLFNFSSRISNKIEIFFS
                     DVIYQKGREIILFITEKFRTFQNGLLPRYIFWIMAGLIFIMEVLKRW"
     gene            7005..7538
                     /locus_tag="PLF90_05405"
     CDS             7005..7538
                     /locus_tag="PLF90_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOK56749.1"
                     /translation="MVTNIFFHVFLLSMMVIGSMVAVFSRKLISSLVSLGTVGMLISI
                     YFLILGAPDIAITQLVVEVLALIILLCAITGRDVTADMTSSKVYVFLFIFIFVISILT
                     FISYSNFIFPEFGNTNMRVGKEYLYLATEKLKSANAVTAIVLDFRGYDTIGEATVIFT
                     AIIGVMVLLRKKGKKDE"
     gene            7531..8004
                     /locus_tag="PLF90_05410"
     CDS             7531..8004
                     /locus_tag="PLF90_05410"
                     /inference="COORDINATES: protein motif:HMM:NF006248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOK56750.1"
                     /translation="MNKGMSEIVKTISKIVVPFIIIFGISVIFYGHLTPGGGFPGGVI
                     ISASFVLLLLAYGREKVLSKLSLYKSDILHDIGAIMFLSVSLIGFAGGVFFLNIFNKG
                     KLFSLWSGGNLLFSNIAIGLKVGTSLFLGITLLSMARIIHKENKIEFYEKEEDIK"
     gene            8001..8351
                     /locus_tag="PLF90_05415"
     CDS             8001..8351
                     /locus_tag="PLF90_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOK56751.1"
                     /translation="MIVYIFIAFMFFIGIYGMMAKKNIIKMIIGMNIIGYAVNLFFIV
                     LGYKSGGIQPILVPGMDIKEFVERAVNPLPQALVLTSIVIDLSIICFLAALSIRIYEK
                     YGTFDLEKIRRLKG"
     gene            8348..9817
                     /locus_tag="PLF90_05420"
     CDS             8348..9817
                     /locus_tag="PLF90_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOK56752.1"
                     /translation="MNLIVFPVILYLLSAFLIALIWKEDEKLSGIACLSFSILVFISV
                     ILLGKYVFLNERIIYFVGNWQIPIGITLMVDALSYIFLVVVNFVLPVICWFSNSYMRK
                     YTKPGYFYILFSLISAGLNGILISMDFFNIYVFLEISSIASYILVAFGLRTEELEASL
                     KYTMIGFIGSTLILIGIGLILGKTGTLNISDFMSAILNINKIQLLFILGLFISGFALK
                     TALFPFHFWLPDAHSLAPSPVSAILSGLFIKVTGFYMLVRIITNIFIIFPESKIIIMA
                     LGLISMIFGSLMAIDQIDIKRTYAYSSISQIGYCALALGIGGYYGYLGAILHFIYHSF
                     SKSLLFLNSGAIEYRYKTTKIEELKGLSDKMPYTTATGSIGMLSISGIPPFGCFWSKI
                     IIIIAAIKSFYYGIAFICVIVSVITLGYFLKLKRNIFDSKGEGEKFEEVNFNLLMPMV
                     ILSILIFFGGLILLPEVKIYLDKAVGVMQNFSYIQIGER"
     gene            9819..10301
                     /locus_tag="PLF90_05425"
     CDS             9819..10301
                     /locus_tag="PLF90_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOK56753.1"
                     /translation="MEFFVIFILTFIVWMILTWNIEFLNIIIGLFVCAFVAFIFRKEF
                     KIDRKVFQIKRYIYFIEYIFVFVYYMIKANILMAYRVLSPRLPIKPGIVKIPIDLKSN
                     LARTILANSITLTPGTFTLKITDEYLYIHWIYIYTYEKGKQKENIVGIMEKILKRVFE
                     "
     gene            10298..10552
                     /locus_tag="PLF90_05430"
     CDS             10298..10552
                     /locus_tag="PLF90_05430"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOK56754.1"
                     /translation="MNTIFIFIGICVAMCVYRIVRGPTAADRSVGIDILGLIIIIYCV
                     FYSLITGIDFYMNIALAWSILGFVSTITLAKFLEGRKFDD"
     gene            10545..10874
                     /gene="mnhG"
                     /locus_tag="PLF90_05435"
     CDS             10545..10874
                     /gene="mnhG"
                     /locus_tag="PLF90_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOK56755.1"
                     /translation="MIELIGWFLIFIGLLFVFLGCLGLVRMPDVYCRLQVSTKCVTLG
                     AGAILFGAFLISGFSSIGFKALLCIVFLFLNSPTAAHALSYGAFVSGVKPVEGTEIEE
                     ELLEGEK"
     gene            10875..11132
                     /locus_tag="PLF90_05440"
     CDS             10875..11132
                     /locus_tag="PLF90_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HOK56756.1"
                     /translation="MHELSVVNNILKIVERVCEENNAKKVLKINIKINPYSCLDEENL
                     NFIFSSITKEKEIYKDTKIEVKRSLNPSEREYIVENIEIEV"
     gene            11135..11794
                     /gene="hypB"
                     /locus_tag="PLF90_05445"
     CDS             11135..11794
                     /gene="hypB"
                     /locus_tag="PLF90_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOK56757.1"
                     /translation="MFKVIKVEESIFEENKKIAEEVKDILEKNKVFSFNVMGAPGSGK
                     TLFIEKTIEILKEEFKFGVIEGDIEGTFDAERFKRFDIPVVQINTGGGCHLDANMVKN
                     GILNLPLEKIEILFIENVGNLVCPAEFEIGTEKNIVVSSITEGENKVSKYPLMFKVSH
                     ICILNKIDLLPYIEFDIEKFKKEIKQISPDTYFIKLSSKTGENFSEWIEYLKGLKREK
                     K"
     gene            11752..12216
                     /locus_tag="PLF90_05450"
     CDS             11752..12216
                     /locus_tag="PLF90_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HOK56758.1"
                     /translation="MDRILERIKKGEKVAIIGIGNRLKGDDAAGSIIAEKLKEKVKNE
                     NLLVIDAEINPENYIGVIKKFNPPFILIIDAIDFGSFPGDFRIFKPEQIKDTTISTHN
                     FSIPLLKNLIDNIEIYILGIQPEKIKLGENISQKVSETIEKIIKTFLVLLPP"
     gene            complement(12642..12818)
                     /locus_tag="PLF90_05455"
                     /pseudo
     CDS             complement(12642..12818)
                     /locus_tag="PLF90_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
     gene            complement(12815..13555)
                     /locus_tag="PLF90_05460"
     CDS             complement(12815..13555)
                     /locus_tag="PLF90_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HOK56759.1"
                     /translation="MPAAFQAFQREFGRYLRDPHHVARPPGLPARRAGIYRDLVFNNL
                     CGFVDTCFPVCRSLLGEPRWRRLCRTFQRDWPLPSPWFRDIPREFVRYLGAADIRQPL
                     PRWLAELAHYEWAELAVDVSDAPIPPCDPTGDLLEHPVVLNPALMNLAYAWPVQRIGP
                     DYRPRQPQPTHLVVYRDAGDRVRFSEVTPVTGRLLDLFASETLSGRQAILRLAEALQH
                     PDPGQWLGFGAALLADLRQQGILLGSQP"
     gene            complement(13555..>13729)
                     /locus_tag="PLF90_05465"
     CDS             complement(13555..>13729)
                     /locus_tag="PLF90_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HOK56760.1"
                     /translation="IDPVWALLTAAYERIGCPVPTCLERDFNIPDLAALSAEVGHIAR
                     LQSQAAGRQRQVA"
BASE COUNT         4386 a         2082 c         2265 g         4996 t
ORIGIN      
        1 ttttaagaaa ttatgactgg ctggacccga taagttttta tgaaaaatca ttaaagtggg
       61 gatttgaaga tggtaaactt tattataatt taggaacaat ttattacaga atgaaaaatt
      121 atgaaaaagc acttgaatta ttttcaaaat ccattgattt attcaaaaat aaaaaacttc
      181 catatctcgg tattgcttcc tgttatgcta acattggaaa atatgatgaa gcaataaatt
      241 attatgaaaa ggttcttgaa atagaccctg ataatccatt tgctttatgt tctattgcca
      301 caatttatta taatcagaga aaaaaatcac ttacagaaat atttgaactt ttagataaat
      361 gtattaaaaa aaatccaact tacagaccat cttatgttct tgccggaaga atattttacg
      421 aaaatgataa ttttaaagaa agtataaaat tttttaaaat tgcagtatca atagacccaa
      481 atgatgacct ctcacattta ttacttggaa taagttattt caatacaggc aataaagaag
      541 aagcagaaaa acatctaaca aaagcatata atctaaatcc aaataattat gaaaatattc
      601 aaaatcttgc ctttttctat aaaataacag gaaaatttga taaagcactc attctctatg
      661 aagaagcaat caaattaaag cctgatgatt tagatacttt aaacgatatt ggtttatgtt
      721 atgcaatgat tggtgaaaaa gaaaaggcaa aaaatatatg ggaagaaata ttaaagaaaa
      781 acccggatta taaacctgca aaaacaaatc ttcaaatact taaaaaaaat aactaattct
      841 ttttaaaaat acctataact gcctgaccga gtgaaatacc accatcattt acaggaactt
      901 tattatgaat cagaacatca aacttttctt tcttaagttt ttttaaagta tcattcagta
      961 aaaacatatt ttgaaaaact cctcctgata gagcaacttt atttatatct ctttcctccc
     1021 tcaaaatttt acacatatcc ttaattattt ctgaaacagt tatatgaaat ctgtatgaaa
     1081 tttcacctgt ttttaatcct tttttcaaat catccgttat tccttttaaa atcatttccg
     1141 gctctatgat ataaccagtt ttttctttat ttattttata ctcataaggt tttaaatttt
     1201 tatttttttc aatcttcatc tcaagttcta ttgctgcttg cccttcataa tcaatcttct
     1261 ctcttatact taaaattgaa cttacagcat caaaaaacct tccaaaactt gagtttaaaa
     1321 aagtatttat attattatca accatcttct ttattatacc ccattttttc atatctatct
     1381 tttttacaaa atctatatct aaattcagga aatcttcccc aaataccata taaagataaa
     1441 ctgttcctat tctccatacc tctttaattg atatttctcc aggtaaaggg aagtatctta
     1501 aatgtccaat cctttcaaaa ttttcataat cacatataag aaattctccc ccccaaattt
     1561 ttccatccag tccatatcca gtcccatcaa aagcaactcc aattaccttt tcatttttta
     1621 agaaattgtc tgccatgcag gaagttatat gactgtggtg atgctgaaca tatatttttt
     1681 ccctatcagg aaaaatttct tctgctattt ttgaagaaaa atatcctgga tgcatatcac
     1741 agacaattat ctctggctca acttcaagaa tgtttttgaa aaggtttatt gactttttat
     1801 atgattcaat tgataattca gtttcaaggt ctcctatatg ctgacttaaa taaacatttt
     1861 tactctttgt aaaagaaaaa gtatttttca tatcacttcc tgctgaaaaa attattttat
     1921 tataagtata aggtatctca acaggtgatg gaacaaatcc tcttgacctt cttataaaat
     1981 aaatcttttt attgaaaatc cttaagacag aatcatcaca gtttatataa atttttctgt
     2041 tatgggtcaa aaaaaagtca catataccac ttaatctttt aaaagcaatc tcatcttcat
     2101 aaataattgg ctcttctgaa aaattaccac ttgtcataac aagaacattt aaattcttac
     2161 caaaatggaa taaaaggtga tgaacaggtg tatagggaag aaaaaatccc aggtaattgt
     2221 tttcaggtgc aatttcataa ggaaggattt catttttctt tttcagaagg acaattggtg
     2281 tctgccagga tttcagcaac tttttttcaa aattatttat atagcagaat ttctctataa
     2341 cttttaaatt tcttgccatt aaagcaaatg gtttcccgta tcttttcttc ctttccctta
     2401 aaaatttaac tgccttttca ttttcagcat cacatgcaag atgataacca ccaattcctt
     2461 taattcccaa tattttcccc attttaatta attctgagcc ctttttaata gcatcaacat
     2521 tttcagcaac ttctttccct ttattatcaa aaagttttaa agttggaccg catgtaaaac
     2581 agcagtttgg ttgggcatga aatcttcttg aataaacatc tttatattcc ttctgacagg
     2641 tatcacacat aataaattca tccatagttg tattttttct atcataagga acttttttta
     2701 caattgtaaa tctcggacca caatcagtac agttaatgaa aggaaaaaga taccttctat
     2761 ttttttgatt gaaaagttct cttaaacatt tttcacaggt tgctatatca ggagatattt
     2821 caacttctct tgtttcttct tttttacttt taagtataaa aaaatctttc tcatttttaa
     2881 tatctatctt tcttatatct atttctttta tctctgcttt tgaaggaggg gattttctta
     2941 ttctttctat aaaatattca attttgtttt tctctccttc tatttcaatc aaaacaccac
     3001 ttcctgtatt acaaacatat cctttcaaac ccaaattttt tgcatatctg tatatagtag
     3061 gtctgaaacc aactccctga acctttcctt ttaccaaaat tctatatctt tcttccaatt
     3121 tcatccttta ataatccttg caaacttctt tcctgcttct ataagttttt cctcatcttc
     3181 tttatttggt gcatttttta taagtatcgg gtctaaaact tttttaaatt taaaacttgc
     3241 agcaattttt tctatacttt caattgcttt tccacctcca ccatgagtta aaaaggcaaa
     3301 atatggtttt ccttcaactt ttccccttga aggataaaaa gtcctgtcaa aaaagtcctt
     3361 cagtcctcca gccatataac tgaaataatc aggcgaacca ataactatac catcacagga
     3421 aagtaaatcg tcaatatctg catctattcc ctttttcagt actacttcaa gcccttcact
     3481 ctcaattcct ttttttataa tttctgctgc tttttcagta tttccaccaa gagtataata
     3541 aacaatcaat atttttctca tttttcacct cctgttttca tttttatttt atcatttata
     3601 ttttttaaat ctaacaagtt ttcaaaaatc tctacatcaa acataatttt ctccttagaa
     3661 gttgataaaa tacaaataga aaaaattttt ctttttcacc tatcttaacc tgaaagaaaa
     3721 ttttacttga caaaaaaaaa tttagggtat attattattt acatttttat ttgtgatttt
     3781 tttcacaaag taaagaaatt ttaaaaatga tactattaat tttacttttg ccttttttag
     3841 gagcattttt gagaaagttt tttccaaaga gattttctct ttttattgca actttaatta
     3901 cacttataga aattttactg attttttatt ttgttcaaat tacagaaaat gtttatgaaa
     3961 tgaagtttga tttgggtggt attttattca tatatgatga ccttggaata atcttttcaa
     4021 ttacatcatg ctttattgct tttttaattt ttctttattc ctttggatac tttaaagagg
     4081 aaaaagaaag ggattatttc agtttctggg gtttgatttt tctcggaagt atgctcggag
     4141 ttattttttc agacaattta atttctcttt attttttctg ggaaattgca ggtctatgtt
     4201 cttatagatt aatcgggttt tatagagaaa aagagcatct tctgaaagca gacaaagcat
     4261 ttatgataac agttgcaggt gctggaatta tgtttttatc ttttgcatac atatattcaa
     4321 ttacagggac tttttctctt tctgaaatga aaggcatttc tgtttctcct tttgtttttc
     4381 ttttgttttt tataggggtt ataacaaaat cagcaacttt tccttttcat acatggcttc
     4441 cagatgcttc agttgctccc actcctgtta ctgcatttct tcatgcagca gttcttgtaa
     4501 aaattggtat ttatggactt ttaaggattt tctggaagac actaacagtt gaaggaaata
     4561 ttgtctgggc attgtatcta tgtatattca gcagtttaat agcaggtatt gttgctttga
     4621 ttgaaaatga ttttaaaaaa attcttgctt attcaactgt aagtcaactt ggatttttaa
     4681 taacaggtat ccttactttt aataatacag gcatatcagg gtctttaatt ttttatattg
     4741 cacatgctct tggaaaagga ggattatttt taactgcagg acttatggaa aaagttttta
     4801 aaacaaaaga tataacaaaa atgggtggag ttatgaaaaa tatgcccttt ttaacttttg
     4861 ggtatctttt ttcaatgttt tctgttatag gaattccacc ttttgctggt ttttttggta
     4921 aaatcaatat cataatgggc gtcttaaaaa atcatttact gtttgctggc ttttttttaa
     4981 ttataagttc aatacttaca cttttatatt tatggagaac atttaagtat gtttttatat
     5041 ttgaaaatgc aacttctaat tttgatttta attataaggg ctcaaaaata atgacttttt
     5101 ctattttaac acttggtctt ttttcttttt taataggtat aatgttattt tttgttaaag
     5161 gaggaatttt tggatattaa attgttattt ttacctgttt ttgtaagttt tggtttttcg
     5221 tttcttaatc ttttatttta tggaattggc aggaatatca gaaaatatct ttctattttt
     5281 ggttctttat tagtactcat ttcatctatt tatattttga aatactctgt cgcccttcat
     5341 tccagtacat taccaaactg ggaaaattta agtatatttt ttgcaatatt aatttctgct
     5401 ataagtttat tctgcataat ttactctcag tggtataatc catctgcaaa ttttgtttat
     5461 gattctttgt ttttcctttt tacaggttca atgcttggtg tagttttatc aggttcttta
     5521 atatattttt atgtcttctg ggaattgatg acaatttcaa gtttctttct tgttctttat
     5581 gaaaatacag aagagtcaaa aaatgcttca ataaaatata ttgtaatgac cggtgctgga
     5641 agtattttcc ttttatttgg aattatagga gtaaatttca ttgaaaaagg gattatatgg
     5701 aagcatttat ttttcttctc aatttttatt ggagcaggtg ttaaaagcgg gcttttccct
     5761 ttatatagtt gggttccaga aacatatcct tcgtcaccaa ccccaataag cgcaatccta
     5821 tcaggtcttg caagcaaaat gggaatatat gcttttatta aattttattt tactattttt
     5881 caaccaggat ggtctattgc ctgggaaaat ttaatgatta ttactggaat gattacactt
     5941 ttatcaggag ttttacttgc tttaatacag catgatatta aaagattgct tgcatatcac
     6001 agtataagcc agattggtta tatcttcctt ggaattgcct gtggaacttc aattggatta
     6061 tgtggaggtc tttatcatct tctaaatcac tcaatattta aatcacttct tttccttgga
     6121 gcaggtatct taataaaaat gacacattca agagaacttg aaaaatatgg aagattttct
     6181 tctatattac catttacttt ttatactttc tcaattgcat cactatctat ttcaggaatt
     6241 ccacctttca atggttttgt ttcaaaatgg ataatatgtc aggcacttct tgaaaagaaa
     6301 acaggtatat caatttttgc aatgataatt tctttacttg gaagtgcttt aacactggct
     6361 tctttcatta aagtattaaa tgatgcattt cttggagttg gaaagggtga aattaaagaa
     6421 aaaattaatg aaaggacacc gcttatcata ataccttttg gatttcttgc ccttggttgt
     6481 atagttttcg ggatttctcc ttcccttgtc ataaataaaa tattcagccc gattatagga
     6541 agtattccaa ctctgaatat aaaacttatt aattttataa gtttctgggg atatctttca
     6601 atacttgttt tatttgccgg ttatatttat ttatcaagac agacacgaaa atttaaagaa
     6661 aagaaagttt ttgttggagg tgaaaacata ccaaaagaaa taactgattt tgacggttct
     6721 catttttaca aaactgttta tgaaacaaat ggtataaagg acttttatga aattgaaagg
     6781 aaaggattat ttgactctta caggatttct gaaaaacttt tcaatttttc aagtaggatt
     6841 tcaaataaaa ttgagatttt tttcagcgat gtaatttatc aaaaaggaag ggaaattatt
     6901 ctatttatta cagaaaaatt caggactttt caaaatggac ttttaccaag atatattttc
     6961 tggattatgg ctggtttgat atttataatg gaggtattaa aaagatggta accaatatat
     7021 tttttcatgt gtttttgctt tccatgatgg taattggttc tatggttgct gtattttcaa
     7081 gaaaacttat atcctctctt gtttctcttg gaacagttgg aatgcttata agtatatact
     7141 ttttaattct tggtgctcct gatatagcaa taacacaact tgttgttgaa gttttggcat
     7201 taattatttt attatgtgca attacaggaa gggatgttac tgcagatatg acttcttcaa
     7261 aggtttatgt ttttctcttc atttttatat ttgtaatttc aattctcact tttatttcct
     7321 attctaattt tatattccct gaatttggaa atacaaatat gcgggttgga aaagagtatt
     7381 tatatcttgc aacggaaaaa ttaaaatcag caaatgcagt gacagcaata gttcttgatt
     7441 tcagaggata tgatacaata ggtgaagcaa ctgttatatt cactgcaatt ataggcgtta
     7501 tggttctttt aagaaaaaaa ggaaaaaaag atgaataagg gtatgagtga aattgtaaag
     7561 acaataagta agatagttgt tccattcatt attatttttg gtatttcagt tattttttat
     7621 ggacatttaa ctccaggagg tggctttccc gggggtgtta taatttctgc ttcatttgta
     7681 cttttgcttc ttgcatatgg aagagaaaag gtcttatcta aactatccct ttataaaagt
     7741 gatattttac atgacattgg agcgattatg tttttatctg tatctttaat cggttttgct
     7801 ggtggtgtat ttttcttaaa tatttttaac aaaggtaaac tcttttcact ttggagtgga
     7861 ggaaatttat tgttcagtaa tattgcaata ggacttaaag ttggaacaag tttattcctt
     7921 ggaataactt tactttcaat ggcaagaata atccataaag aaaacaaaat tgaattttac
     7981 gaaaaggagg aagatattaa atgattgttt acatatttat tgcttttatg ttttttatcg
     8041 ggatatacgg aatgatggct aaaaaaaata taattaagat gattattgga atgaatataa
     8101 taggttatgc agtcaattta ttttttatag ttcttggtta taagagcgga ggaattcagc
     8161 caatacttgt tccggggatg gatataaaag aatttgtaga aagagcagta aatcctttac
     8221 ctcaggcact tgttttaact tccatagtta tagatttatc aattatatgt tttcttgctg
     8281 ctttatcaat aagaatttat gaaaaatacg gaacttttga ccttgaaaaa ataaggaggt
     8341 taaaaggatg aatttaattg tttttccagt tattttatat ttactttcag catttttgat
     8401 tgcacttata tggaaagaag atgaaaaact atcagggatt gcctgtttat ctttttcaat
     8461 ccttgttttt atatctgtta ttcttcttgg aaaatatgta tttttaaacg aaagaattat
     8521 atattttgtt ggtaactggc agattccaat aggtataact ttaatggtgg atgctctttc
     8581 ttatatattc cttgttgttg ttaattttgt tttacctgtt atttgctggt tttccaattc
     8641 ctatatgaga aagtatacca aacctggcta cttttacatt cttttctccc tgatatctgc
     8701 tggattaaac ggtattttaa taagtatgga ctttttcaat atatatgtct tccttgaaat
     8761 ttcttcaatt gcttcataca ttcttgttgc ttttggattg agaacagaag aacttgaagc
     8821 gtctcttaaa tatacaatga ttggttttat cggttcaact ctaatactta ttggaattgg
     8881 tttgattctc gggaaaacag ggactttaaa tataagtgat tttatgagtg caatattaaa
     8941 tataaataaa atacaattgt tgttcatact tggactgttt atttctggct ttgctctaaa
     9001 aaccgctctt ttcccgtttc atttctggct tcccgatgct cactcacttg ctccttcacc
     9061 tgtttcagca atactttctg gtttatttat taaagttaca ggtttttata tgcttgtaag
     9121 aataatcaca aatatattta taattttccc tgaaagtaaa attataataa tggctcttgg
     9181 acttatttct atgattttcg gttctctgat ggcaatagac cagatagata taaagagaac
     9241 ttatgcatat tcaagtataa gccagattgg ttattgtgca cttgctcttg gaataggtgg
     9301 atattacgga tatcttggtg ccattttaca ttttatttat cattctttct caaaatctct
     9361 tctcttttta aattcaggag caattgaata cagatataaa acaacaaaaa ttgaggaatt
     9421 aaaaggactt tctgacaaaa tgccatatac aacagcaacc ggttcaatag gtatgctttc
     9481 tatttcagga attccacctt ttggttgttt ctggagtaaa attataatta taattgctgc
     9541 tattaagtca ttttattacg gaattgcctt tatatgtgtt attgtttcag taataaccct
     9601 tggatatttt cttaaactta agagaaatat ttttgacagt aaaggtgaag gagaaaaatt
     9661 tgaagaagtt aactttaatc ttttaatgcc aatggttatt ttaagtattt tgattttttt
     9721 tggaggacta attttacttc cagaagttaa aatttatctt gataaagcag ttggtgtaat
     9781 gcaaaatttt tcttatatac aaatagggga gagatgatat ggaatttttt gtaatcttta
     9841 ttttaacttt tattgtatgg atgattttaa catggaatat tgagttttta aatataatca
     9901 taggtctttt tgtatgtgct tttgttgcat ttattttcag aaaagagttt aaaattgaca
     9961 gaaaggtatt tcagataaaa aggtatatct atttcattga atatattttt gtttttgttt
    10021 attatatgat taaagcaaat atccttatgg cttacagggt tttaagtcca agattaccaa
    10081 taaagccagg tatagttaaa ataccaattg atttaaaatc aaatcttgca agaaccatac
    10141 ttgcaaactc tataacatta acaccaggaa cttttacact taaaataaca gatgaatatc
    10201 tttacattca ctggatttat atttatactt atgaaaaggg aaaacagaag gaaaacatag
    10261 ttggaattat ggaaaaaatt ttaaaaaggg tctttgaatg aatacaattt ttatatttat
    10321 cggaatttgt gttgctatgt gtgtttatag gatagtcaga ggacctactg ctgcggatag
    10381 aagcgttggt attgatatac tcggattaat tattatcatt tactgtgtat tttattctct
    10441 tataacagga attgattttt atatgaacat tgctcttgcc tggagtattc ttggctttgt
    10501 gagtacaata acacttgcta aatttcttga agggaggaaa tttgatgatt gaacttatag
    10561 gatggttttt aatatttata ggacttttat ttgtatttct cggttgcctt ggtcttgtca
    10621 gaatgcctga tgtttactgc cgtcttcagg tatctacaaa atgtgtcact cttggtgctg
    10681 gagcaattct ttttggtgca tttttaataa gtggtttttc aagtattggt tttaaagcat
    10741 tactctgtat tgtctttttg tttttaaatt ctccaaccgc tgctcatgct ctatcctatg
    10801 gtgcttttgt ttcaggagta aaacctgttg aagggacaga aatagaagag gaattacttg
    10861 aaggagaaaa atgaatgcat gaactctctg ttgttaacaa tattcttaaa atagttgaaa
    10921 gagtttgtga ggaaaataat gcaaaaaaag ttcttaaaat aaatatcaaa ataaatcctt
    10981 acagttgtct tgatgaagaa aatttaaatt ttattttttc ctcaataaca aaagaaaaag
    11041 aaatttataa ggatacaaaa attgaggtaa aaaggagttt aaacccgtct gaaagagaat
    11101 acatagttga aaatattgaa attgaggtat agaaatgttt aaagtcataa aggttgaaga
    11161 aagtattttt gaagaaaata aaaaaattgc agaagaggta aaagatattc ttgaaaaaaa
    11221 taaagttttt tcttttaatg taatgggtgc tcctggaagt ggaaaaacat tatttattga
    11281 aaaaacaata gaaatactta aagaagaatt taaatttgga gttattgaag gagatattga
    11341 aggaacattt gatgcagaaa gatttaaaag atttgatatt ccagttgttc agataaatac
    11401 aggaggtgga tgccatcttg atgcaaatat ggtaaaaaat ggaatattga atttaccact
    11461 tgaaaaaatt gaaatactct ttattgaaaa tgttggaaat cttgtttgtc ctgctgaatt
    11521 tgaaataggt acagaaaaaa atattgttgt ctcttcaatt acagaaggcg aaaataaagt
    11581 ttctaaatat ccccttatgt ttaaagtttc tcatatctgt attttaaata aaattgacct
    11641 tctcccatat attgaatttg atattgaaaa atttaaaaaa gaaattaaac aaatatcacc
    11701 tgatacatac tttataaaac tttcttcaaa aacaggtgaa aacttttctg aatggataga
    11761 atacttgaaa ggattaaaaa gggagaaaaa gtagcaatta ttgggattgg aaacagatta
    11821 aaaggggatg atgctgctgg aagtataatt gctgaaaaac taaaagaaaa agtaaagaat
    11881 gaaaatttac ttgttataga tgcagaaata aatcctgaaa attacatagg agttattaaa
    11941 aaattcaatc ctccttttat actcataata gatgcaattg atttcggttc ttttccagga
    12001 gattttagga tttttaaacc agagcagatt aaggatacaa caatttcaac ccataatttt
    12061 tcaataccac ttttaaagaa tttgatagat aatattgaaa tttatatact tggaattcaa
    12121 ccagaaaaaa taaaacttgg tgaaaatata agtcaaaaag tatcagaaac aatagaaaaa
    12181 attattaaaa cctttttagt ccttcttcca ccttaaaggt ggactttgga aattaagaca
    12241 ggataaggtt tttgaagggt aattttttgt ggtaaaattc aattcaaatg ggatactgta
    12301 agatatgcaa aaaggaaaaa ttactttcag aaaatcttgg tatctgtctt gaatgtataa
    12361 gacaaaaacc agaagaagca atttctctgg cagattatgt tcataaaaaa gtaagagaag
    12421 gatttaatct tccagcattc cctccaaaaa ctgaaaatgg atttaaatgt gaattatgtt
    12481 ttaacaggtg ttcaattggt aaaaaagaaa aaggatattg tggattaaga gaaaacttta
    12541 atggtttttt aaaaagtaaa gtcagtcagg ataaaggagt ccttgatttt tattatgacc
    12601 caattccaac aaattgctgt gctgaatggt tctgtggtgc agatattgaa gggaaaggga
    12661 aagcgttcct gaatgtcggc gaaccagttg gtgccgcgga atttttccag gccggactcg
    12721 aaataatccc agccgagcag gacacgcagg ctgagcaggc cgagccacac gccgaggagg
    12781 tcgatcgcat cgaagctgcg caggatgaac ttgttcatgg ctggctcccg agaaggatgc
    12841 cttgctgccg cagatcggcc agcagtgcgg cgccgaaacc gagccattgg ccgggatcgg
    12901 gatgttgcag cgcttcggcc aaacgcagga tggcctgccg gccgctcagg gtttcactgg
    12961 cgaacaggtc gagcaggcgc ccggtgaccg gcgtcacttc gctaaagcgc acgcggtcgc
    13021 cggcatcgcg atagaccacc agatgcgtcg gctgcggctg gcggggccgg tagtcggggc
    13081 cgatgcgctg caccggccag gcgtaggcca ggttcatcag ggccggattg aggacgaccg
    13141 ggtgttccag cagatcgccg gtcgggtcgc agggcggaat cggcgcatcg ctgacgtcga
    13201 ccgccagttc ggcccactcg taatgcgcca gctcggccag ccagcgcggc agcggctggc
    13261 gtatgtcggc tgcgccgagg tagcgcacga attcacgggg aatgtcgcgg aaccacggtg
    13321 acggcagcgg ccagtcgcgc tggaaggtac ggcacaggcg gcgccagcgg ggctcgccga
    13381 gcaggctgcg gcacaccggg aagcaggtat cgacgaagcc gcacaggtta ttgaagacca
    13441 ggtcgcggta gatgccggcc cggcgcgccg gcaggcccgg cgggcgggcg acgtgatgcg
    13501 ggtcgcgcag gtagcggccg aattcgcgct gaaacgcctg gaaagcggcc ggcatcacgc
    13561 cacctgcctt tggcgcccgg cagcctggga ctgcaggcgg gcgatgtggc cgacttcggc
    13621 gctcagcgca gccagatcgg ggatattgaa atcgcgctcc aggcaggtcg gcaccgggca
    13681 gccgatgcgc tcgtaggcgg ccgtcagcaa cgcccagacc gggtcgatc
//
LOCUS       DAOSAT010000049        13429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3115_length_13429_cov_5.566547, whole genome shotgun sequence.
ACCESSION   DAOSAT010000049 DAOSAT010000000
VERSION     DAOSAT010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3115_length_13429_cov_5.566547"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="PLF90_05470"
     CDS             <1..1392
                     /locus_tag="PLF90_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56761.1"
                     /translation="LTLDDIERILSLTIKRDKEVKIITFLGMITAYTDDAQVNIAFIS
                     PASTGKSHIPLEIAKYFPEGDVIEIAYASPTAFFHTTDKRYVEYIREEYLKKINFERK
                     ILVFIDQPHSELLERLRPLLSHDKKNLVIRITDKNKKGQFRAQNIMLVGFPVVIFCSA
                     NTFMNEQESSRLILISPQMDEEKIKEVLEYKIEKEANENFNQNIENNPERLSLKRRIE
                     VIRNLRFKGVRIEKCELIDKYLEERKKEGLVSIDTRNIDKLYSIIKALTLLNFPYREI
                     KGKTLIANRKDIEIGLNLWKEVEKYQNFGIPPYIYWFYEQVVIPVYKKKMKENKELKG
                     IKKKDLLSYQIKGGLQKLISEKRLRIEILPTLINSGFIREEKEKLKKLGIKLLSPLKA
                     IKEYCLRCCYGDRKYLKECPSVNCSVWEYRLGKNPCLKGKRKNNLRPNHQKYGQKIQV
                     DEAFFKNKDEIFV"
     gene            1436..1699
                     /locus_tag="PLF90_05475"
     CDS             1436..1699
                     /locus_tag="PLF90_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK56762.1"
                     /translation="MAEKIQKRLLTIKEASEYLGIQVKTLYKWVYEKKIPYVKLSHKS
                     LKFDKIKLDEWIDRQTIHPVNIEEKVDKILSLVYNKKQKNESI"
     gene            1686..2249
                     /locus_tag="PLF90_05480"
     CDS             1686..2249
                     /locus_tag="PLF90_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56763.1"
                     /translation="MRVFKRDGYWWIDFTFQGKRHRKKISKKKREAEKALASIHTRII
                     EGKYLEIKRNEKIKFEELTQTYLNKYSRINKKSFRRDITSIKNLNTYFKGKYIYEIKG
                     IDIENYKTKRINEGVKCSTINRELGCLRAMLNKAVEWGLLKTIPPKIKLFKENNQIVR
                     YLKPEEAKKIIDLAPEPLKSIIIIAQV"
     gene            2246..2773
                     /locus_tag="PLF90_05485"
     CDS             2246..2773
                     /locus_tag="PLF90_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOK56764.1"
                     /translation="MRRGEIVNLKWQDIDMKERIITLWDTKSKEKRYVPMNEIVFQTL
                     LNIEKDPLSEYVFYGKDKNSHISEHYISHLFEKIIKKAGITNFRFHDLRHTFASWLVM
                     KGVNLKTVQELMGHKDFTTTLRYAHLSPEHKKLAVEILSDEIKFYLEEQKSGTYLALN
                     RKEEKSEVVKTLENT"
     gene            2775..2849
                     /locus_tag="PLF90_05490"
     tRNA            2775..2849
                     /locus_tag="PLF90_05490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2810..2812,aa:Arg,seq:cct)
     gene            3071..3361
                     /locus_tag="PLF90_05495"
     CDS             3071..3361
                     /locus_tag="PLF90_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HOK56765.1"
                     /translation="MKTFVMLSTLTEKGAKTVKTKPERIKEVNKEVEKMGGKILSQYA
                     ILGPFDFLTIISAPNEETVAKISLEISQRGSVKITTYTAIDVDEFINNIKEK"
     gene            3358..4131
                     /locus_tag="PLF90_05500"
     CDS             3358..4131
                     /locus_tag="PLF90_05500"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HOK56766.1"
                     /translation="MKCRLIISDNYNPFFNMAFDYYFWQKCIDKKNLPVLRFYKWNPS
                     SVSTGYNQKKESLINLEFCEENKIPIVMRPTGGSAIFHDIEITYSFCANTSHHTFFSS
                     PFSSYIILCKGIIKGIEKKGINFKIRGFSEGEEPSFTDIPCFSLSSRHDIVYNDKKVI
                     GSAQRRNNFSFLQHGSILIDIRKDLWENIFIKEVDFSKIGCLKDIISDIDEKELMRFL
                     KEGFEEVLSFSIFEDQLKKEEIEEIKIIEEKLKKEGKYE"
     gene            4124..4867
                     /locus_tag="PLF90_05505"
     CDS             4124..4867
                     /locus_tag="PLF90_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK56767.1"
                     /translation="MNKFMENLGVQSYCFRNFKDNREVAKMVRELGLNKIELCGVHIN
                     FLDESKFKEVISIYEDEGVKIYSIGVQGFSNNEANERKYFEFVKMCGAKYISADFDIN
                     KIFDCFRVAEKLADEYDVKLAIHNHGGWHWLGNIQTLDYVFKNTSERIGLCLDTAWSL
                     DSRIDPVKMIETFDKRLYSLHLKDFIFERDRRPVDVVLGEGNIDIGKIVNKLKEINFQ
                     GPLIIEYEGEPENPVPSIKKGIENFKKYL"
     gene            4886..6511
                     /locus_tag="PLF90_05510"
     CDS             4886..6511
                     /locus_tag="PLF90_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOK56768.1"
                     /translation="MEVKGVIFDFDGTLVFLSIDFKKIKEKILKEAEKLNLKIPSENL
                     PILELLEKIKKLNGEKGEKFYSLGHRILKTEEIKASEKTYPKKGVMDLLVKLKEKGIK
                     IGIITRNCREVVEKVVDRFSIPYDIILTRDDVKKYKPDPLHIKECLKKMGLQKNEVIL
                     VGDHFLDVRCGKKLNILSIGIAGEYVKEEVFLKEGADFVFYDIEDIEYILNLKGFPSG
                     KLPNKFLKYLIERYTAKDKGILILPGVGIDCAIFRMKDKYVYAKADPITLTSKDIGFY
                     LVNINVNDISVMGGVPEWFLCVLFFPENTVFSDIEYVFSQISNECKKFNIKWIGGHTE
                     IISGIEKVIAVGFMIGSKIKRVKFLKIKQGDKIFLVKEIGIEGASIIAREKFDELKKY
                     FSEKYLKKVKNSIKNPGISVFKETKLMWENFKIKWMHDPTEGGISTALYEISESKKIG
                     IVVNLKKLKFYSPVIKFCKVLGFNPLGIISSGCILGIIDRKEEKKLIDFCKKNRIKLQ
                     IIGEVIKEKGVYYIKNSKKYRFLKFNRDEINKL"
     gene            6630..7001
                     /locus_tag="PLF90_05515"
     CDS             6630..7001
                     /locus_tag="PLF90_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK56769.1"
                     /translation="MEIKKILIIEDDPEERLILSRELKRIGYIVYEAATGEEGLKIFK
                     EDKPDLVILDVVLPGIDGWEVLKRIKKGPLSRKVPVMMLTGKSENEDKIKGYEIGADY
                     YVTKPYNVNKLLPVIKSLIEE"
     gene            7004..7972
                     /locus_tag="PLF90_05520"
     CDS             7004..7972
                     /locus_tag="PLF90_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOK56770.1"
                     /translation="MVVNLPPQFHEKEAELKKAKTIEEKISILEEMLAIMPKHKSSEK
                     LQALLKSKISKYKKMLEEQTKTKKKSPVPVIQREGAGQIGICGPPNSGKSTLLSKLTK
                     ANPEIGDYPFTTKIPTPGMMDYQDIKIQVIDTPSLSIEFTENWVGDILRKADSLIFLF
                     DISSDFILEEMEDSLKVLEKFKIKEEGEFKFNKNIIWVGNKIDLPESKDIKEIFFEFY
                     SDKIPEVITISAKNTENIEILKEKMFISLDIIRVYTKTPGKPPDLENPYTLKKNTTVI
                     ELAEIIHKDLLKNFKYARLWREGNDKVIIAGRDYILQDRDIIEIHD"
     gene            8064..8396
                     /gene="rpsF"
                     /locus_tag="PLF90_05525"
     CDS             8064..8396
                     /gene="rpsF"
                     /locus_tag="PLF90_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOK56771.1"
                     /translation="MLRNYELCFLIKADLPEEEFNKEVDFIERVLLREGAEIVKKDNL
                     GRKTLAYPIKKKEEAIYYLFYIKTKPENISKIESNFYRRENILRYLILQRKKLNFEEE
                     VNAGSVSE"
     gene            8374..8769
                     /gene="ssb"
                     /locus_tag="PLF90_05530"
     CDS             8374..8769
                     /gene="ssb"
                     /locus_tag="PLF90_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOK56772.1"
                     /translation="MPGPFLNKVFLIGRLTRDPELRYTTSGTAVATFGFAVNREYLSK
                     GERKEDTCYLNLVVWGKQAEICAEYLKKGNLIFVEGSLHYRSWETEDNEKKSTIEVRV
                     ERFQFLEKPNVEIIEEKEEMENGKEDQDL"
     gene            8744..8956
                     /gene="rpsR"
                     /locus_tag="PLF90_05535"
     CDS             8744..8956
                     /gene="rpsR"
                     /locus_tag="PLF90_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOK56773.1"
                     /translation="MAKKTKICKFCQENIDEIDYKDIGRIRRYITPRGKIISSKQSGV
                     CSKHQRALAKAIKRARFVGLLPFVKI"
     gene            8965..9414
                     /gene="rplI"
                     /locus_tag="PLF90_05540"
     CDS             8965..9414
                     /gene="rplI"
                     /locus_tag="PLF90_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOK56774.1"
                     /translation="MKVIFIKDYQNKGKKGEIKEIKDGFARNFLIPYGYALEATESNI
                     KILKEKEKMEETKKKRKIEQAKELKNILKESSVTIQAKAGRDEKLFGAITSEIISEEI
                     KKQLNIDIERHQIILEEPIKKLGIYKVPVRLAEGLEGEIKVWIIREK"
     gene            9416..10771
                     /gene="dnaB"
                     /locus_tag="PLF90_05545"
     CDS             9416..10771
                     /gene="dnaB"
                     /locus_tag="PLF90_05545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOK56775.1"
                     /translation="MPKKKEEIPVDKIPPQNIEAEKALLGCMLLDEEARIKVLENFKV
                     DFFYSEAHQKIFSSIVKLFEKGEKCDLITLTEILKGENALKEIGGVEYLTHIVETVPT
                     SAYVDDYMKIVREKYILRSLISNATKIISEAYNQQDEVETLLDKAESLIFEVSQNKIE
                     REAYPLKNLIRDAIENIEKIQDKRGYVTGLPTGFIDLDKMTTGLHPSDFIIIASRPSM
                     GKTALACNIALNLNSGVEKIPVLIFSLEMSKEQIVQRMLCSEAKVNILKLRQGLLSDK
                     EIGRLLIAASQLEEASIFIDDTPSLNVFELRARARRLKAKENIQVIIIDYLQLMRGKG
                     RAENRQQEISEISASLKSLAKELNIPVIAVSQLSRATEQRVDKKPQLADLRESGSIEQ
                     DADLVLLLYREEYYNETEENKGIAEVIIAKQRNGPTGSVFLTFLEEYTRFENYTPRQE
                     I"
     gene            10768..11541
                     /locus_tag="PLF90_05550"
     CDS             10768..11541
                     /locus_tag="PLF90_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK56776.1"
                     /translation="MKKFLENLGEKFLFLINYIGSVSILIFSSTKKITKFKKKEDILK
                     FILEIGNESLFLVGIISLFTGMVLVMETVHTLKKFGAEMYSGGLVTISMIRELGPVLI
                     ALIIAGRIGASIAAEIGVMKITEQIDALEVMAVDPVVYLVTPRVIAGIISLPCLFILS
                     FFISIIGGFFVGVFMVGIPSGIYLYQSFKYVNIKDFLIGLLKVVVFAVIIVNVSSYEG
                     LNAYGGAEGVGRASTNAVVSSFFLIILANLIITGLFYFV"
     gene            11543..12283
                     /locus_tag="PLF90_05555"
     CDS             11543..12283
                     /locus_tag="PLF90_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK56777.1"
                     /translation="MEKIISVKNLNVKLGNKDVLKDINFDIEKGESIVIVGPSGCGKT
                     VLIKTILGIIKRYTGEIYIFGKNMRILKEEELMEIKGKMGMVFQNSALFDSLTVWENV
                     GFYYLYHTDKSKEEIKNLAIESLKSLGLDESVADMMPEQLSGGMKKRVSIARALISKP
                     QIIFYDEPTTGLDPITSENLTELIRKIHQNFNTTDITVTHDVKLAGKIGKRLILIDEG
                     KIIESGTYEELREKSKHPIIRSYIETGG"
     gene            12285..13400
                     /locus_tag="PLF90_05560"
     CDS             12285..13400
                     /locus_tag="PLF90_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOK56778.1"
                     /translation="MEKKITDELKVGIFIFAAIIGIIVFIFTQTRLGKWTGYEIGVLF
                     DYVGGLETGSPVRVSGVRVGEVKKIEIIYEETPKVFVKLKINPNVKIGKHSRFTIKTL
                     GIIGEKYVEINPSNEKEFIKKGEIVEGENPLSVDKIANMGEEIAKNLNKILSDISQIT
                     GDKKIQEQIREILASFKTTFDRLNDSFTKIDGLVEGLSKTNNNLNSFIEENSSKISKV
                     IDDTDLFLVSGKSEIEKTMGSIRDFLTIKDKADKLLVNFTETSEEFQKTSSQIKEFFE
                     KLQTEGLIAKIMKEEKMLEDIKEEIALLKETTEKIGDASEKLSITLENLNSVLVDLKS
                     GKGSAGKFLYSDELYNEALNFIKDIREHPWKLFFRRK"
BASE COUNT         5473 a         1384 c         2523 g         4049 t
ORIGIN      
        1 cttacacttg atgatattga gagaatttta agtttaacaa ttaagcggga caaagaagta
       61 aaaattatca catttttagg gatgataact gcatatactg atgacgctca agtaaatatc
      121 gcttttatat caccagcaag cacagggaaa agtcatattc ctttagagat agcaaaatac
      181 tttccagaag gtgatgttat agaaattgct tatgcctccc caacagcatt ttttcatact
      241 actgataaga gatatgttga gtatatcagg gaagaatatt taaaaaaaat aaattttgaa
      301 agaaagattt tagtatttat tgaccagcct cattcagagt tattggaaag attaaggcct
      361 ttattaagcc atgataagaa aaatttggtt attagaataa cagataaaaa taaaaaagga
      421 caattcagag cccagaatat tatgttagtt ggtttccctg tagtcatttt ttgttcagca
      481 aatactttta tgaatgaaca ggagagttca aggcttatct taataagtcc ccagatggat
      541 gaggagaaaa ttaaagaagt attggaatac aaaatagaaa aagaggcaaa tgaaaatttt
      601 aaccagaata tagaaaacaa tcctgaaagg ctgtcattaa aaaggagaat tgaagttata
      661 agaaatttaa gatttaaagg agttagaata gagaaatgtg aattaattga taaatattta
      721 gaggaaagaa aaaaagaagg attagtttca atagatacaa gaaacattga taaattgtat
      781 tcaattatca aagctttaac actcctaaat ttcccatata gagaaataaa agggaaaact
      841 ttaatagcaa atcgaaagga catagaaatt gggctcaatt tatggaagga agttgagaaa
      901 taccaaaatt ttggtattcc tccttacatt tactggtttt acgaacaagt agttattcca
      961 gtatataaaa agaaaatgaa agaaaataaa gaattaaaag ggattaaaaa gaaggattta
     1021 ttaagttatc aaattaaggg gggattacaa aaacttataa gtgaaaaaag attaagaatt
     1081 gaaattttac caacacttat aaactctggg tttattagag aagaaaaaga aaaacttaaa
     1141 aaattaggaa ttaaattatt gtctccttta aaagccataa aggagtattg tttaaggtgt
     1201 tgttatgggg atagaaagta tttaaaagaa tgtccaagtg taaattgttc tgtatgggaa
     1261 tataggttag gaaaaaatcc ctgtttaaaa ggaaagagaa aaaataattt aagacctaac
     1321 catcaaaaat atgggcaaaa aatacaagtt gatgaggcat tttttaaaaa taaagacgag
     1381 atttttgttt agtatggggt tatccctaaa aaaatagggg gtatttttta aaaaaatggc
     1441 agaaaaaata caaaaaagat tactaacaat taaagaagca agtgaatatc ttggaataca
     1501 agtaaaaaca ttatacaagt gggtttatga aaagaaaata ccttatgtta aactctccca
     1561 caagtcattg aaatttgata aaattaaact tgatgaatgg attgatagac aaacaatcca
     1621 tccagttaat atagaagaaa aagttgataa aatcttaagt ttagtatata ataaaaaaca
     1681 aaaaaatgag agtatttaaa agagatggtt attggtggat agattttaca tttcaaggga
     1741 aaaggcatag aaaaaaaata agtaagaaaa aaagagaagc agaaaaagct ttagcatcaa
     1801 tccatacaag aataatagaa gggaaatatt tagagattaa aagaaatgaa aaaataaaat
     1861 ttgaagaact tacccaaact tatcttaata aatattccag aattaacaaa aaaagtttta
     1921 ggagagacat tacaagcata aaaaatctta atacttactt taaaggaaaa tacatttacg
     1981 aaataaaagg aatagacata gaaaactaca aaacaaaaag aataaatgaa ggagtaaaat
     2041 gttcaacaat aaatagggaa cttggatgtt taagagcaat gttaaataaa gcagttgaat
     2101 ggggattatt aaaaacaatt cctccaaaga ttaaattatt taaagaaaat aaccaaattg
     2161 taagatattt aaaaccagag gaagcaaaaa aaataataga tttagcacct gaaccattaa
     2221 aaagcataat aataattgca caggtatgag gagaggagaa atagtaaatt taaaatggca
     2281 ggatattgat atgaaagaaa ggattataac tttatgggat acaaaaagta aagaaaaaag
     2341 atatgttcct atgaatgaaa ttgtattcca gacactttta aatattgaaa aggacccttt
     2401 aagtgaatat gttttttatg gaaaagataa aaactcccat ataagtgaac attatatatc
     2461 ccatttattt gaaaaaatta ttaaaaaagc aggtataaca aactttagat ttcacgattt
     2521 aagacataca tttgcaagtt ggcttgtaat gaaaggagta aatttaaaaa cagttcagga
     2581 attaatggga cataaagatt ttacaacaac attaagatat gcacacttat cccctgaaca
     2641 taaaaaactt gcagttgaaa tattgagtga tgaaataaaa ttttatttag aagaacaaaa
     2701 aagtggcacc tatttggcac tgaacagaaa agaggaaaaa tcagaagttg tgaaaaccct
     2761 tgaaaatact taaagcccct gtagctcagg aaggatagag cagcggtttc ctaaaccgcg
     2821 tgtcgcccgt tcaagtcggg ccaggggcat attgaaaaat agaggcaaac agaataattg
     2881 aaaatttgga ggaagatata aaaagggcga cacaaagggg ttatagggga aaggaatgtc
     2941 ccctatgttt ttcgttttgg ggggagaatt tcaggaaggg ggaagcaccc cctcctgaga
     3001 aaagtcgccc gttcaagtcg ggccaggggc atatatttga tactgttaaa aaaagagggg
     3061 aggtgagaaa atgaaaactt ttgtaatgtt aagtaccttg actgaaaaag gagcaaaaac
     3121 tgttaaaaca aagccagaga gaataaaaga agtgaataaa gaggttgaga aaatgggtgg
     3181 gaaaatttta tctcaatatg ctattcttgg accgtttgat tttttaacca ttatttctgc
     3241 tccaaatgaa gaaactgttg caaaaatatc tcttgaaata tctcaaagag ggagtgtaaa
     3301 aataacaacc tatacagcaa tagatgtgga tgaattcata aataatataa aagaaaaatg
     3361 aaatgcagat taattatatc tgataattac aaccctttct ttaacatggc ttttgattat
     3421 tatttctggc agaagtgtat tgataaaaaa aatttacctg ttttaaggtt ttataaatgg
     3481 aatccgagtt ctgtatcaac aggttataat cagaaaaagg agagtttgat taatcttgag
     3541 ttttgtgaag aaaataaaat tccaatagtt atgagaccaa caggaggaag tgccattttt
     3601 catgatatag aaattactta ctctttctgt gcgaatactt cccatcatac ttttttttct
     3661 tctccatttt cttcatatat aattctatgc aaagggataa tcaaagggat agaaaagaaa
     3721 ggaataaatt ttaaaataag aggattttca gaaggggaag agccatcctt tactgatata
     3781 ccctgttttt ctctatcttc caggcatgat atagtttaca atgataaaaa agtaattggt
     3841 tctgctcaga gaagaaataa tttttctttt ttacagcatg gttcaatttt aattgatata
     3901 agaaaggatt tgtgggaaaa tatatttatt aaagaggttg atttttctaa aattggttgc
     3961 ttgaaggata taatttcaga tattgatgaa aaagaattaa tgagattttt gaaagaaggt
     4021 tttgaagagg ttttgagttt cagtattttt gaagaccagt taaaaaaaga agaaattgaa
     4081 gaaattaaaa ttatagaaga aaaactaaaa aaggagggaa agtatgaata agtttatgga
     4141 aaatcttggc gttcagagtt attgtttcag aaattttaag gataatagag aagttgcaaa
     4201 gatggtaaga gaactgggtt taaataaaat tgaattatgt ggagttcata taaattttct
     4261 tgatgagagt aaatttaaag aagttatttc aatttatgag gatgaaggag ttaaaattta
     4321 ttccattgga gttcagggtt tttcaaataa tgaagcgaat gagagaaaat attttgaatt
     4381 tgtaaaaatg tgtggagcaa aatacataag tgctgatttt gatataaata aaatttttga
     4441 ctgttttaga gttgctgaaa aacttgctga tgaatatgat gttaaacttg caatacataa
     4501 tcatggtgga tggcactggc ttggaaatat acagacactt gattatgtct ttaaaaatac
     4561 ttcagaaaga attggactct gtcttgatac tgcatggtca cttgattcaa gaatagaccc
     4621 agtaaaaatg attgaaacat ttgacaaaag attgtattcc cttcatttaa aggattttat
     4681 ttttgagaga gatagaagac ctgttgatgt tgttcttgga gaggggaata ttgatatagg
     4741 taaaattgtg aataaactta aagagataaa ttttcaggga ccgcttataa ttgaatatga
     4801 gggagaacca gaaaatccag tcccatccat taaaaaagga attgagaatt ttaaaaaata
     4861 tctttaatca tcaaggagaa aagttttgga agtaaaggga gtaatttttg attttgatgg
     4921 gacacttgta tttctttcta ttgattttaa gaaaataaaa gaaaagatac ttaaagaagc
     4981 agaaaaacta aatcttaaaa ttccttctga aaatttacca attcttgaat tacttgaaaa
     5041 aataaaaaaa ttgaatggag aaaagggaga aaaattttat tctttagggc atagaatttt
     5101 aaaaactgaa gaaattaaag catctgaaaa gacatatcct aaaaaagggg ttatggattt
     5161 acttgtaaaa ttaaaagaaa aaggtataaa aataggaata attacgagaa actgtagaga
     5221 agtggttgaa aaagttgttg ataggttttc cattccttat gatattattc ttacaagaga
     5281 tgatgttaaa aaatataaac cagacccttt acatataaaa gaatgtttga aaaagatggg
     5341 attacaaaaa aacgaggtta ttcttgtagg tgaccatttt ttagatgtaa gatgcgggaa
     5401 aaaattaaat atattgagta ttggaattgc tggtgaatat gtaaaagaag aagttttttt
     5461 gaaagagggt gctgattttg ttttttatga tattgaagat attgagtaca ttcttaattt
     5521 aaaaggtttt ccttcaggta aacttccaaa taaattttta aagtatctga ttgaaagata
     5581 tacagcaaaa gataagggga tattgattct gcctggtgtg gggattgact gtgcgatttt
     5641 tagaatgaaa gataaatatg tttatgcaaa ggcagaccca ataactttaa cttcaaagga
     5701 tattggtttt tatcttgtga atattaatgt aaatgatatt tctgtaatgg gtggagttcc
     5761 tgaatggttt ttatgtgttc ttttttttcc ggaaaataca gttttttctg atattgaata
     5821 tgttttttcc cagataagta atgaatgtaa gaaatttaat ataaagtgga taggtggaca
     5881 tactgaaata atttctggta ttgaaaaggt tattgcagtt ggatttatga ttggaagtaa
     5941 aataaaaagg gtaaaatttt taaaaataaa acaaggagat aaaatttttc ttgtgaaaga
     6001 aatagggata gagggtgctt caattatagc aagagaaaag tttgatgagt taaaaaaata
     6061 tttttcggag aaatatttaa aaaaggttaa aaattcaatt aaaaatccag ggataagtgt
     6121 atttaaagaa acaaaattaa tgtgggaaaa ttttaaaata aaatggatgc atgacccgac
     6181 agaaggagga atatcaaccg ctttatatga aatttcggaa agtaaaaaaa taggaattgt
     6241 agttaattta aaaaaattga agttttattc acctgttatt aagttttgta aagttttagg
     6301 atttaatcca ctgggtataa tttcttccgg ttgtattctc ggaataatag acaggaaaga
     6361 agaaaaaaaa ttgattgatt tttgtaaaaa aaacagaatt aaactacaaa ttataggcga
     6421 agttataaag gaaaaaggag tatattacat aaagaacagt aaaaaataca gatttctaaa
     6481 attcaatagg gatgagataa ataaattgtg aaatagaaat tttggaaaat ctttttagta
     6541 tttttaatcg tcgtttttcc ttaaattgta aaaatttaaa ggaattaaaa taaatagaca
     6601 cttgaattta catgtggtaa aataataata tggaaataaa aaaaattttg attattgaag
     6661 atgacccgga agaaagattg attttaagca gagagttaaa aaggatagga tatattgttt
     6721 atgaagcagc aaccggagaa gaaggattga aaatttttaa ggaagataaa ccagaccttg
     6781 tcattcttga tgttgtacta ccaggtattg atggatggga agttttaaaa cgaattaaaa
     6841 agggtccttt gagcagaaaa gttcctgtta tgatgcttac aggaaaaagt gaaaatgaag
     6901 ataaaataaa aggatatgaa attggcgctg attattatgt aaccaaacca tacaatgtta
     6961 ataaattact tcccgttata aaaagtttga ttgaggaata aagatggttg taaatcttcc
     7021 tccacaattt catgaaaaag aagcagaatt aaaaaaagca aagacaattg aagaaaaaat
     7081 ttcaattctt gaagaaatgc ttgccataat gccaaaacat aaatcaagtg agaaacttca
     7141 ggcactttta aaaagtaaaa tatcaaaata taagaagatg cttgaagagc agacaaaaac
     7201 aaagaaaaaa tcacctgttc ctgtgataca gagggaagga gccggtcaaa taggaatatg
     7261 tggtccacca aattcaggta aatccactct tctctcaaaa ttaacaaaag caaatccgga
     7321 aataggtgat tatcctttta caactaaaat accaacccca ggaatgatgg attatcagga
     7381 tataaagata caggtgattg atactccttc tttatctatt gaatttacag aaaactgggt
     7441 gggtgatatt ttaagaaaag cagactcttt gattttttta tttgatattt caagcgattt
     7501 tatacttgaa gaaatggaag attcattaaa agttcttgaa aagtttaaaa taaaagaaga
     7561 gggtgaattt aaatttaata aaaatattat atgggtggga aataaaattg atttaccgga
     7621 aagtaaagat ataaaagaaa ttttttttga attttactct gacaaaatac ccgaagtaat
     7681 aactatttct gcaaaaaata ctgaaaatat tgaaatccta aaagaaaaaa tgtttatttc
     7741 tcttgatata atcagagtat atacaaaaac acctggaaaa ccacctgacc ttgaaaatcc
     7801 atacacttta aagaaaaata caacagttat tgaacttgca gaaattattc ataaagacct
     7861 tcttaaaaat tttaaatatg caagattgtg gagagaggga aatgataagg tgataattgc
     7921 tggaagggat tatattcttc aggatagaga tattattgaa attcatgatt agttaatttt
     7981 tattgacttt tttaaatttc taaatataat taaattatag acggtaaata ataaatcatc
     8041 ctaaatggaa ggaggaaaaa aatatgttga gaaattatga attatgtttt ttaataaaag
     8101 cagatttacc ggaagaagaa tttaataagg aagttgattt tattgaaaga gttcttttaa
     8161 gagaaggtgc agaaatagta aaaaaggata atcttggtag aaagacactt gcatatccaa
     8221 ttaaaaagaa ggaagaggca atttattatc ttttttatat aaaaacaaaa ccagaaaata
     8281 tctcaaagat tgaaagcaat ttttacagaa gagaaaatat tttgagatat ttgattttac
     8341 agagaaagaa gttaaatttt gaggaggagg ttaatgccgg gtccgtttct gaataaagtt
     8401 tttttaatag gaagattaac aagagaccca gaattaaggt ataccacaag cggtactgct
     8461 gttgctactt ttgggtttgc ggttaataga gaatatctat caaagggaga aaggaaagaa
     8521 gatacttgtt atttaaatct tgttgtctgg ggaaaacagg cagaaatatg tgctgagtat
     8581 ttgaaaaaag ggaatttgat atttgttgag ggttctttac attacaggag ttgggaaaca
     8641 gaagataatg aaaaaaaatc aactatagaa gtcagagttg aaagatttca gtttttagaa
     8701 aaacccaatg ttgagataat agaagaaaag gaggaaatgg aaaatggcaa agaagaccaa
     8761 gatttgtaaa ttctgtcagg aaaatattga tgagattgat tataaagata taggaagaat
     8821 aagaagatat ataactccaa ggggaaaaat tatttcctca aaacagtcag gggtctgttc
     8881 aaaacatcaa agagctcttg cgaaggcaat aaaaagagca agatttgtag gacttctacc
     8941 gtttgttaaa atatgaggtg aaaaatgaaa gttatattta taaaagatta tcaaaacaaa
     9001 ggtaaaaaag gagaaataaa agaaataaaa gatggatttg caagaaattt tttaattcct
     9061 tatggatatg ctcttgaagc aacagaaagc aatattaaaa ttttgaaaga aaaagaaaag
     9121 atggaagaga cgaagaaaaa gagaaagatt gagcaggcaa aagagttaaa aaatatattg
     9181 aaagaaagtt ctgttactat acaggcaaaa gcaggtagag acgagaaact ttttggagca
     9241 ataaccagtg aaataataag tgaggaaata aaaaaacaac ttaatattga tatagaaaga
     9301 catcaaataa tacttgaaga accgataaaa aaactgggta tatataaagt tcctgtaaga
     9361 ttggcagagg gtttagaagg tgaaataaag gtctggataa taagagaaaa ataaaatgcc
     9421 taaaaaaaag gaagaaattc ctgttgataa aattcctcca caaaatatag aagcggaaaa
     9481 agctctttta ggatgtatgc ttcttgatga agaggcaaga attaaagttc ttgaaaattt
     9541 caaggttgat tttttttaca gtgaagcaca tcagaaaatt ttttcttcaa tagtaaaact
     9601 ttttgaaaaa ggagaaaaat gcgaccttat aaccttaaca gaaatattaa aaggagaaaa
     9661 tgcacttaaa gaaattggtg gagtagaata tcttacccat attgttgaaa ctgttccaac
     9721 atctgcatat gttgatgatt atatgaaaat agtcagagaa aaatatatat tgaggtcttt
     9781 aatctcaaat gctacaaaaa ttatttctga agcatataat cagcaggatg aagttgagac
     9841 acttcttgac aaagcagaat cattgatatt tgaagtaagc cagaataaaa tagaaaggga
     9901 agcgtatcct ttaaagaacc ttattagaga tgcaattgaa aacatagaaa aaattcagga
     9961 taagagaggt tatgttacag gccttccaac tggctttatt gaccttgaca aaatgacaac
    10021 aggtcttcat ccatctgatt ttattattat tgcttcaaga ccatcaatgg ggaaaactgc
    10081 tctcgcctgt aatatcgcac ttaatttgaa ttcaggagta gaaaaaattc cagttttaat
    10141 tttttccctt gaaatgagca aagaacagat tgttcagagg atgttatgca gtgaggcaaa
    10201 ggttaatatt ttgaaattaa ggcagggact tctatctgat aaagaaatag gtagattact
    10261 aattgctgca tctcaacttg aagaagcatc aatttttatt gatgatactc cttctttaaa
    10321 tgtttttgaa ttgagagcaa gggcgagaag attgaaagca aaagaaaata ttcaggttat
    10381 aattattgat tatttacaac ttatgagagg aaaaggaagg gctgagaata gacagcagga
    10441 gataagtgaa atttcagcat ctttaaaatc acttgcaaaa gagttgaata tccctgtgat
    10501 tgcagtttct cagttaagca gggctactga acaaagagtt gataaaaaac cacaacttgc
    10561 agatttaaga gaaagtggtt caatagaaca ggatgcagac cttgttttac ttttatacag
    10621 ggaagaatat tataatgaaa cagaagaaaa taagggtata gcagaagtta taatagcaaa
    10681 gcaaagaaat ggtccaactg gttctgtctt tcttacattt ctggaagaat acacaagatt
    10741 tgaaaactat acaccaagac aggaaatatg aaaaaatttt tagaaaatct tggtgaaaaa
    10801 tttttgtttc ttattaacta tataggttca gtttcaattc ttatcttttc ttcaacaaaa
    10861 aagataacaa aatttaagaa aaaagaggac attttaaagt ttatacttga aattggaaat
    10921 gagtcattgt ttcttgttgg aataatatct ttatttacag gaatggtact tgttatggaa
    10981 acagttcata ctcttaaaaa atttggtgct gaaatgtatt caggagggct tgttacgatt
    11041 tcaatgatta gagaacttgg tcctgtttta attgctttaa ttattgctgg tagaataggc
    11101 gcttcaattg ctgcagaaat aggtgttatg aaaattacag aacagataga tgcattagaa
    11161 gttatggctg ttgaccctgt tgtatatctt gtaactccaa gggttattgc aggaattata
    11221 tcccttccct gtctttttat tttaagtttt tttatttcaa ttattggcgg tttttttgtg
    11281 ggtgtcttta tggttggtat tccttcgggt atttatctgt atcagagttt taaatacgta
    11341 aacattaaag attttcttat aggactttta aaagttgtag tttttgcagt tattattgtt
    11401 aatgtttctt cttatgaagg acttaatgct tatggtggag cagaaggagt tggaagggct
    11461 tcaacaaatg cggttgtatc atcgtttttt ttaattattc ttgcaaattt aattataaca
    11521 ggtctttttt attttgtata aaatggaaaa aataatttct gttaaaaatt tgaatgtaaa
    11581 acttgggaat aaagatgtat taaaagatat aaattttgat attgaaaagg gagaatctat
    11641 tgttatagtt ggtccatctg gctgtggaaa gacggtttta ataaaaacaa ttcttgggat
    11701 aataaaaaga tacacaggag aaatttatat ctttggcaaa aatatgcgta ttcttaaaga
    11761 agaagaattg atggaaataa aaggaaaaat gggaatggta tttcagaact ctgctttatt
    11821 tgattcttta actgtctggg aaaatgtcgg gttttattat ctttatcata cagataaaag
    11881 taaggaagaa ataaaaaatc ttgccataga aagtttgaaa tcccttggac ttgatgaaag
    11941 tgttgctgat atgatgcctg aacaattaag tggtggtatg aaaaaaaggg tgtcaattgc
    12001 aagggcttta atatcaaaac ctcaaataat attttatgat gagcccacta caggccttga
    12061 cccaataaca agtgaaaatc ttactgaatt gataagaaaa atacatcaaa attttaatac
    12121 aacagatata actgtaacac atgatgttaa acttgcagga aaaataggta aaagattgat
    12181 tttgattgat gaaggtaaaa taatagaaag tggtacatac gaagaattga gggaaaaaag
    12241 taagcatccg ataataagaa gttatattga aacgggagga taaaatggaa aagaaaataa
    12301 cagatgaact gaaggttggg atatttattt ttgctgcaat tatcggaatt attgttttta
    12361 tctttactca gacaagattg ggaaaatgga ctggttatga aataggagtt ttatttgatt
    12421 atgttggtgg gcttgaaaca ggaagtccag tgagggttag tggagtaagg gttggtgagg
    12481 ttaaaaaaat tgaaattata tatgaggaaa caccaaaagt ttttgtaaaa ttgaaaatta
    12541 atccaaatgt aaaaatcggg aaacacagta gatttacaat taaaacactt ggaattattg
    12601 gggaaaaata tgttgagata aacccgtcaa atgaaaaaga gtttattaaa aagggagaaa
    12661 tagttgaggg agaaaatcct ttatctgtgg ataaaattgc aaatatggga gaagaaattg
    12721 caaaaaattt aaataaaata ctttcagata tcagccaaat tacaggtgat aaaaaaattc
    12781 aggagcagat aagagaaatt cttgcaagtt tcaaaaccac gtttgataga ttgaacgatt
    12841 cttttacaaa aatagatggt cttgttgaag ggctatctaa aacaaataat aatttaaatt
    12901 cttttattga agaaaattct tcaaagattt caaaagttat tgatgataca gatttatttc
    12961 ttgtttcagg gaaaagcgaa attgaaaaaa cgatgggaag tataagagat tttttgacaa
    13021 ttaaagataa agcggataaa cttcttgtta attttacaga gacatcagaa gaattccaaa
    13081 aaacatcctc acagattaaa gaattttttg aaaaactcca gacagaagga cttatagcaa
    13141 aaattatgaa agaagagaag atgcttgaag atattaaaga agaaatagca cttttaaaag
    13201 aaacaactga aaaaattgga gatgcatctg aaaaattgag tattaccctt gaaaatttaa
    13261 attcagtttt agtagattta aaatcaggta aaggcagtgc aggtaagttt ctatatagtg
    13321 atgaacttta taatgaggct ttaaatttta taaaagacat aagagaacac ccctggaaat
    13381 tattttttag aagaaaatga aaaaagaaaa aagtatattt gtatgttct
//
LOCUS       DAOSAT010000050        12949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3256_length_12949_cov_5.650302, whole genome shotgun sequence.
ACCESSION   DAOSAT010000050 DAOSAT010000000
VERSION     DAOSAT010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12949
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3256_length_12949_cov_5.650302"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..716
                     /locus_tag="PLF90_05565"
     CDS             99..716
                     /locus_tag="PLF90_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK56779.1"
                     /translation="MYKKTIKYFFKLLIQGVFIFLFFYFFKKTKFPLSEKLPLNFFFR
                     IDLLFAIFTTISTLHFSYLFLPSIIILFLLLFLGNFFCFWVCPLGGIIDYANIIFLRK
                     KWKIGIKIPKWVRYIKVFIFWAIIFTSFTAIFLKIPFLFWMFDPYVILMRGLLFKKIL
                     IILIVIIFLNILIPRFWCYYICPFGYLNYLIGVKLRNKIKRKIKK"
     gene            713..1297
                     /locus_tag="PLF90_05570"
     CDS             713..1297
                     /locus_tag="PLF90_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOK56780.1"
                     /translation="MKRREFLKFIFGVGGCFIFTGFRFKLTEKSNFIRPPGAIEEDDF
                     IYKCIRCGECLKVCPVSCLRPVPLNKGIFEFGTPHIIPREKACLLCLNCGKICPTGAI
                     QKVKIEEVKMGTAKININKCLVWKENKDCFVCMEVCPVAAIYKNENEKPVVNPEICVG
                     CGQCEQNCPVYGEGAIKVFIKGEKRKKLKGGRHV"
     gene            1290..1469
                     /locus_tag="PLF90_05575"
     CDS             1290..1469
                     /locus_tag="PLF90_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOK56781.1"
                     /translation="MFNIGAPELILIFFIILLLFGAKRLPEIGRSLGKALGEFKKGMK
                     EGVSEEEKKEEKKEE"
     gene            1473..2180
                     /gene="tatC"
                     /locus_tag="PLF90_05580"
     CDS             1473..2180
                     /gene="tatC"
                     /locus_tag="PLF90_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOK56782.1"
                     /translation="MEEKDLSIVEHLEELRRKIIYSSIFFIFSSFISYFFSNKLLLFI
                     VKPLKKYQETIVFLKPVEPFFSILKITFFTAGVISLPFILLQIYLFIAPGLTEKERKI
                     IKTIFIIFPFLFILGMSFTFYIIIPFGLKVLFSFGKDIMKPLISITNYLNFVFVFFIA
                     MGLIFNLPVFISSLSYIGIINSKFLREKRKFAIAGSFILSAIITPTTDIFTQILIAIP
                     LIFLYEISIFLSKIFKN"
     gene            complement(2533..3201)
                     /locus_tag="PLF90_05585"
     CDS             complement(2533..3201)
                     /locus_tag="PLF90_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HOK56783.1"
                     /translation="MRKKIIEAFKQPIQKKNIFDLVIGGVLMFFPVINFISMGYISER
                     LKAGILLEKKTIKWDENIKNLFVKGAYLFFLILGYLLIPFIFLFLGVFFISILSRGEI
                     VSLFLFRGLILIILSTILFLIAIYFLLFGICVYLEENSLKKGFNLRDVLEKIFLIPKD
                     YIIIYILIVGILMISIVILTLLLNWVTGLLLSGFLFFYDLLVISNILIKFYPRKAVNI
                     QLPF"
     gene            complement(3182..3916)
                     /locus_tag="PLF90_05590"
     CDS             complement(3182..3916)
                     /locus_tag="PLF90_05590"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOK56784.1"
                     /translation="MIKLGVNVDHIATLRQARKGRYPDPVEGAILAELAGCDSIVCHL
                     REDRRHIQERDIYLLRKVVKTKLNLEMALHEDIIKIAIDVKPNQVTLVPEKREELTTE
                     GGLDVIKFSEKIKENIKIFQKNGIKVSLFIEPDFSQIEESKRVGADFIEIHTGRYADA
                     VNEDEMYNELNKIIKATEYSISIGLRVNAGHGLDYKNVGKICKIKGIEELNIGYSIIC
                     RSIYVGIYQAVKEMKEIIERNEKENN"
     gene            complement(3967..4707)
                     /locus_tag="PLF90_05595"
     CDS             complement(3967..4707)
                     /locus_tag="PLF90_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56785.1"
                     /translation="MTEIKIDANGLHYKILNEKIRELISKGYKKIYIGNVFGQRYIGA
                     GLREKDVEIYINGTPGNDLGCFMDGPKIFVNGNAQDGTGNTMNSGKIIIKGNAGDILG
                     HSMRGGEIYVKGDVGYRVGIHMKAYKNFYPVIVIGGIAKDFLGEYMAGGLIIILNLKE
                     KKKIVGELTGTGMHGGEIFIKGEIDERTIGKEVKIFKPEKEDIEKIKFYIQKFSEYFG
                     EKLRYIPSNNFIKLIPVSKRPYGKIYAY"
     gene            complement(4704..5996)
                     /locus_tag="PLF90_05600"
     CDS             complement(4704..5996)
                     /locus_tag="PLF90_05600"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK56786.1"
                     /translation="MRNKKRYIIIGNSASGVSCIEGIRENDKEGEILVFSDEDYFNYS
                     RPLISYYLAGKLKEDQLNFKDEKFYEKNNVKLFLKTRCEKIDLKDKILFTTYGKFNFD
                     YIFIGTGGKPIIPDIKGIEKIKNGLFTFTKLNDAKKLERYIEDKKVKEVVILGGGLIG
                     LKCAEGLISKNIFVKIVELSDRIFANTFDNIASEIVENKLKENNCEIIKGNTIEKVNT
                     KDGDLKEVILKDGKKIKANVLVIAIGVVPNTEIIEGINIKKNKGIIVDEYMKTNIPFI
                     YAGGDVAESIEFDGNISVIAIWPRATKQGKIAGINMSGGSKKYEGIFIMNSIEFFGIP
                     TISFGITNPKDEKRCEILKKIDYEKKIYKKIVINENKIVGGIFLGDIERAGILNGLIK
                     NRIDVSSFKNLLLDEKFGLLILPETYRKHIVKGEGIEV"
     gene            complement(5986..6450)
                     /locus_tag="PLF90_05605"
     CDS             complement(5986..6450)
                     /locus_tag="PLF90_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015918778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOK56787.1"
                     /translation="MVKRIIIREEYCIGCRLCEVHCITKHSKTKDIIKTYKGHEPKPV
                     SGIYFEELGHISFAIQCRHCEDAQCIEACLTGAMYRNENTKAVLHNKEKCIGCWMCVM
                     VCPLGVIKIDKENKKVASKCDLCIEEGFPACVENCPNEAIILVNEKGERIEK"
     gene            complement(6444..7964)
                     /locus_tag="PLF90_05610"
     CDS             complement(6444..7964)
                     /locus_tag="PLF90_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOK56788.1"
                     /translation="MAKVKKISEFVVDRDLEKCIKCQACVRMCSNDVHIYDEEFDIVR
                     NDNSKCVGCHFCESICPTEAIEIKRNTSDIKENYHWKKEYIVDIQKQAETGGVILTGM
                     GCDKHYKNYFDHILLNASQVTNPSIDPLREPMELKTFIGRKPDRIEYEFKNGNLILKT
                     KIPPQLELETPIMFSAMSFGSISLNAMKSLAKAAKDFGTYWNTGEGGLPEELYEYADR
                     CIVQVASGRFGVNIDYLEKAGAIEIKIGQGAKPGIGGHLPGEKVTEQISKTRMIPEGT
                     DALSPAPHHDIYSIEDLKQLIFALKEATSYKKPVGVKIAAVHNVAPIASGIVRAGVDY
                     IVIDGVRGGTGAAPKIIRDNVGIPIEIAIAVVDQRLREEGIRNNASIIAAGGFRKSSD
                     VIKAIALGADAVYIGTAALISLGCHLCQKCYTGKCNWGIATQDPYLTKRLNPEIGAKR
                     VYNLLRAWSLEIKEMLGGMGINAIESLRGNREQLRGIDLNEIELKMLGIKHAGEAW"
     gene            complement(7952..9043)
                     /locus_tag="PLF90_05615"
     CDS             complement(7952..9043)
                     /locus_tag="PLF90_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56789.1"
                     /translation="MDDRICKIPSGCGVSGIISKNGNLFSGERIILSICNMVERGNGL
                     GSGFAGYGIYPEFSDKWCFHIMYNSEKDIETCEDYLEANFEIIHSESIPTNKNHKLKN
                     IPIMWRYFLKIKEEKINEEAKSTEEDFIVKTVMFINKKIESAFVISSGKNMGIFKGVG
                     NPDEIADFFKIYDYKGYLWISHNRFPTNTVGWWGGAHPFGILNWAVVHNGEISSYGIN
                     RRYLENFGYYCTLFTDTEVITYLFDLLYRKHKIDFETIGNILASPFWFQIERIEDENC
                     KNFLKSLRIIYGPGLVNGPFGIIVANSEYMVGLNDRIKLRPLVAGEDEDFYYLSSEES
                     AIRIISKDLKNVYEIEAGKPVIFKIKWQK"
     gene            complement(9046..10377)
                     /locus_tag="PLF90_05620"
     CDS             complement(9046..10377)
                     /locus_tag="PLF90_05620"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOK56790.1"
                     /translation="MDKSLKKEDVLKICKEKDVKFIRLFFSDITGQMKGFSIPVEELE
                     GALEDGMGFDGSSIKGFARIDESDMVALPDPKTFTILPYRPKEKAVAAMICDILNPDR
                     TPYEGDSRYILKKNLEYMKEKGYTFYVGPELEFFIFKSDKMTEILDNGGYFDVATLDA
                     ATDVRREIILTLEEMGIKVEYSHHEVAPSQHEIDLRYTDALTMADIVMFYRMIAKEIA
                     SKYGFYITFMPKPIYGVNGSGMHTHQSLFKGEKNAFFDPKDEFYLSDIAKKYIAGLLK
                     HAKEITFVCNQWVNSYKRLVPGYEAPVYICWARRNRSTLIRVPMYRPGKEKSTRIEFR
                     SPDPVCNPYLAFACMVKAGMKGIEGNYKLPEPLEMDVYHLTEEDRKRLGLDVLPGSLI
                     EAIEIAEKSQLLKETLGEHIYTNLLTAKKIEWDEYRKIVHEYEIKTYLPIL"
     gene            complement(10379..11041)
                     /locus_tag="PLF90_05625"
     CDS             complement(10379..11041)
                     /locus_tag="PLF90_05625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOK56791.1"
                     /translation="MKQFKKCLIIKHKIEEGPGIIEDILKEMGILYKIIELENGEKLP
                     EDIKNIDFIFSLGGPMNVYQEREYPFLKEEEDLIKKIYQFKKHFLGICLGAQILAKTF
                     GAKVNLNFCKEIGFFEVELTENGKKDNIFTGVSETFTCFQYHSDCFELPENGILLVKG
                     KKCLNQGFKIGENLYGFQFHIEINKKLLFDFGVKKVYYKKLEKIKETGEIIIKNFLKK
                     EG"
     gene            complement(11028..12092)
                     /gene="nadE"
                     /locus_tag="PLF90_05630"
     CDS             complement(11028..12092)
                     /gene="nadE"
                     /locus_tag="PLF90_05630"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOK56792.1"
                     /translation="MDNLFIGLCQLNFIVGDINGNTEKIIRTIEKAKELKCNFLCFPE
                     LSITGYPPEDLLLKNKFIQDNLKAVERIKNKVEDMVVIVGFVNREDSKIYNSAAIIYK
                     RKIIGIYNKKILPNYDVFDEKRYFDEGKKEGIYEINGIKFGITICEDIWHKNDPYAKQ
                     IKDGAKYIFTLNASPYHFRKIKERENLVRNICTENNVFLFYTNLVGGQDELVFDGQSF
                     AMNPKGEIIKKASPFEEEILFFSTNEKQEKIIEISEEEEIYKALICGLKDYVYKNNFK
                     KVVIGLSGGIDSSLVATIATDALGKDNVIGVFMPSRFSSSQSFNDAKKLAENLGIKFI
                     VIPIEDAIRIRTGEKGDEAI"
     gene            12694..>12949
                     /gene="pyrG"
                     /locus_tag="PLF90_05635"
     CDS             12694..>12949
                     /gene="pyrG"
                     /locus_tag="PLF90_05635"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305926.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HOK56793.1"
                     /translation="MKKFIFITGGVVSSLGKGIASASIGTLLESHGYKVTFLKFDPYI
                     NVDPGTMSPFQHGEVYVTEDGTETDLDLGHYERFTNAKMTH"
BASE COUNT         4295 a         2227 c         1406 g         5021 t
ORIGIN      
        1 tacacttctt ggtaaaaagc cagaacagat tcctcacata aaaattggtg ctgaaatggg
       61 tctcggtaca atgaatcttg acaagataaa aataaaatat gtataaaaaa acaatcaaat
      121 atttttttaa attgttaata cagggtgtat ttattttcct ttttttttat ttttttaaaa
      181 aaacaaaatt tccactttct gaaaaactac ctttaaattt cttttttcgg atagatttac
      241 tttttgcaat tttcacaact atttcaactc ttcatttttc ctatttattt ttgccttcaa
      301 taatcatttt atttttactt ttatttttag gaaatttttt ttgtttctgg gtttgtcctt
      361 taggtggaat aattgattat gcaaatataa tctttttaag gaaaaaatgg aagataggta
      421 taaaaatacc aaagtgggta agatatatta aagtttttat tttttgggct ataattttta
      481 catcttttac tgcaatcttt ttaaaaattc cttttttatt ctggatgttt gacccatatg
      541 taattttaat gagaggacta ctttttaaga aaattttgat tatattaatc gtgataattt
      601 ttttaaatat tttaattcca agattttggt gttattatat atgtcctttc ggttatttaa
      661 attatctaat tggtgtaaaa ttaagaaata agataaaaag gaaaattaaa aaatgaaaag
      721 aagggaattt ttaaaattta tttttggagt agggggatgt tttattttca cagggttcag
      781 atttaagtta acagagaaat caaattttat aagacctccg ggagcaatag aagaggatga
      841 ttttatttat aaatgtataa gatgtggaga atgtttaaaa gtatgtcctg taagttgttt
      901 aagacctgta ccattaaata aaggtatatt tgaatttgga acacctcaca taataccaag
      961 agaaaaagca tgtctattat gtttaaactg tggtaaaatc tgtccaacag gagcaattca
     1021 aaaggttaaa atagaggaag taaaaatggg aaccgctaaa ataaatataa ataaatgtct
     1081 tgtatggaaa gaaaataaag attgttttgt atgtatggag gtctgtcctg ttgcagcaat
     1141 atataaaaat gaaaatgaaa aacctgttgt taatccagaa atatgtgttg gttgtggcca
     1201 atgtgaacaa aattgtccag tatatggaga aggtgctata aaagtattca ttaaaggcga
     1261 aaaaagaaaa aaactaaaag gaggaaggca tgtttaacat aggagcccct gaacttatcc
     1321 ttattttctt tattatttta ctactgtttg gtgcgaaaag attaccggaa ataggcaggt
     1381 ctctcggaaa agcacttgga gagtttaaaa aaggaatgaa agagggagtt tcagaggaag
     1441 aaaaaaagga agagaagaaa gaagaatgaa aaatggaaga aaaagattta agtattgtag
     1501 aacatctgga agagttgaga agaaaaatta tttattccag tatatttttc attttttcat
     1561 catttatttc ttattttttt tcaaataaac tacttttatt tatagtaaaa ccactaaaaa
     1621 aatatcaaga aactattgtc tttttaaaac ccgtagaacc attcttttca attctaaaaa
     1681 ttacattttt cacagcaggg gttatatcat tgccttttat tctactccag atttatctct
     1741 ttattgctcc cggtttgaca gaaaaagaaa ggaaaattat caaaactata tttataatat
     1801 ttcctttttt atttattctt ggaatgagtt ttacctttta tattattatt cctttcggct
     1861 taaaagtcct tttttctttt ggaaaggata ttatgaaacc tcttatttca ataacaaatt
     1921 atctaaattt tgtttttgtt ttctttattg ctatgggtct tatttttaat cttcctgttt
     1981 tcatttcctc tttatcttat ataggcatta tcaactctaa atttttaaga gaaaaacgaa
     2041 aatttgcaat agcaggttct tttattcttt ctgcaataat aactccaaca acagatattt
     2101 ttacacagat tcttattgca attccactaa tttttttata cgaaatttca atttttttat
     2161 ctaaaatatt taaaaattaa gtaattgact gtcaaaaact tttattaaac aatttaataa
     2221 aaatttttaa aaataaattt taaaaaattt tgcttcttaa tttctaattt ttatataatt
     2281 atttctggag tttctggaat aaattaaaaa gtccggttgc tgaaaaataa acaaaagtta
     2341 acataatatt tcttatccga cattttttat gcaaatgatt atacatcaat aatttaaaac
     2401 ttttttcatt tttaaaaaag taatggatag ttttctctaa ttaatttcca gaattgaaat
     2461 cttaaaaagt ttttttagag ttttttctac caatacaggc taaaatttag aagatttgaa
     2521 aaatcatctt atttaaaaag gtaactgaat attaaccgct tttcttggat aaaattttat
     2581 aagaatattt gaaataacaa gaaggtcata aaaaaataga aaaccactta ataaaagtcc
     2641 tgttacccag tttaataata aagtgagtat aacaatagaa atcattaaaa tgcccacaat
     2701 aagtatatat attatgatat aatcttttgg tatgagaaaa atcttttcaa ggacatctct
     2761 taaattaaaa ccctttttta atgaattttc ctcaagatat acacatatac cgaacaaaag
     2821 gaaataaatt gcaattaaaa aaagtatggt tgatagtatg attaaaatta gccctctaaa
     2881 taaaaataaa gacacgattt cccctctgga aaggatggat ataaagaaaa cccctaaaaa
     2941 aaggaaaata aagggaatta aaaggtatcc taaaattaaa aaaaacaggt atgctccttt
     3001 tacaaataag ttcttaatat tttcatccca tttaatagtt tttttctcca gaagaatacc
     3061 tgcttttaat ctttctgaaa tatatcccat cgaaataaag ttaattacag ggaaaaacat
     3121 taaaactccg ccaattacaa ggtcaaatat attttttttc tggatgggtt gtttaaatgc
     3181 ttcaattatt ttctttctca tttctctcta taatttcttt catttctttc actgcctgat
     3241 atattcctac ataaattgac ctgcaaatta tactgtatcc aatatttaat tcttcaatac
     3301 ctttaatttt acaaattttt ccaacatttt tatagtcaag accatgtcct gcatttactc
     3361 ttaaacctat tgaaatggaa tattcagttg cttttatgat tttattcaat tcattataca
     3421 tttcatcttc atttactgca tcagcatatc ttcctgtatg tatttctatg aaatcagccc
     3481 ctactctttt gctttcttca atctgactaa aatctggctc aataaataaa ctgactttaa
     3541 ttccattttt ctgaaaaatt tttatatttt cttttatttt ttctgaaaac tttatcacgt
     3601 caagaccacc ttcggttgtt aattcctctc ttttttcagg aaccaaagtc acctggttag
     3661 gtttaacatc aatagcaatt tttattatat cttcatgaag agccatttca aggtttaatt
     3721 ttgttttaac tacttttctc aataaataaa tatccctttc ctgaatatgc cttctgtctt
     3781 ctcttaaatg gcaaacaatt gaatcacatc cagcaagttc cgcaagtata gcaccttcaa
     3841 cagggtcagg atatctccct tttcttgcct gtcttaatgt cgctatatgg tcaacattta
     3901 ctccaagttt tatcatttta atccctttta ttctaatttt atcttatatt caaagaagtg
     3961 taaatattaa taagcataaa tttttccgta gggtcttttt gagacaggaa ttaattttat
     4021 aaaattattt gaaggaatat accttaattt ttcaccaaaa tattcactga atttttggat
     4081 ataaaattta attttttcaa tatcttcttt ttccggctta aatattttta cttcttttcc
     4141 aatggttctt tcatctattt caccctttat aaaaatttct cctccatgca taccagttcc
     4201 tgtaagttct ccaactattt ttttcttttc tttcaagttt aaaataatta tcaatcctcc
     4261 tgccatatat tctccaagaa aatcttttgc tattcctcca atgacaataa caggataaaa
     4321 atttttatat gctttcatat gaattccaac cctatatcca acatctcctt ttacataaat
     4381 ctcccctccc ctcattgaat gtccaagtat atcgcctgca tttcctttta taattatttt
     4441 tccagaattc attgtatttc ctgttccatc ctgggcattt ccatttacaa aaattttagg
     4501 accatccata aaacatccaa ggtcatttcc aggagttcca tttatatata tttcaacatc
     4561 tttttctctt aaaccagccc ctatatatct ttgtccaaaa acatttccaa tataaatttt
     4621 tttgtatcct ttacttatca attctcttat tttttcattt aatattttat aatgaagtcc
     4681 atttgcatct attttaattt cagtcatacc tcaattcctt ccccttttac aatatgtttt
     4741 ctataagttt caggaagaat taaaagacca aatttttcat caagtaataa gtttttaaaa
     4801 gaagagacat caattctatt tttgattagt ccatttaata tccctgccct ttcaatatca
     4861 ccaagaaaaa ttcctccaac aattttattt tcattgataa caatcttttt ataaattttc
     4921 ttttcataat ctattttttt taatatctca catctttttt catcttttgg atttgttatt
     4981 ccaaaagaaa ttgttggaat accaaaaaat tcaattgaat tcataataaa tataccttca
     5041 tattttttac tcccacctga catattaatt cctgcaattt ttccctgttt tgttgctctc
     5101 ggccatattg caataactga gatatttccg tcaaattcaa tactttcagc aacatctcca
     5161 cctgcataaa taaaaggtat atttgtcttc atatattcat ctactattat ccctttgttt
     5221 ttctttatat ttataccttc aataatttca gtatttggta caacaccaat tgctataacc
     5281 aatacatttg cttttatttt tttaccatcc tttaaaatta cttcttttaa atccccatcc
     5341 tttgtattaa ctttttcaat agtatttcct tttattattt cacaattatt ttcttttagt
     5401 ttattttcaa ctatctctga agcaatatta tcaaaagtat ttgcaaaaat cctatcagat
     5461 aattcaacaa ttttaacaaa aatatttttt gagattaaac cttctgcaca tttaagacca
     5521 ataagtccac caccaagaat aaccacttct ttaacttttt tatcttctat atatctttca
     5581 agttttttag catcatttag tttcgtaaaa gtaaataatc catttttaat tttttcaatc
     5641 ccttttatat ccggtattat tggttttcct ccagttccta taaaaatata gtcaaaatta
     5701 aatttcccat aggttgtaaa taaaatttta tcttttaaat caattttttc acatcttgtt
     5761 tttaaaaata gttttacatt atttttttca taaaactttt catctttaaa gttcaattgg
     5821 tcttctttaa gttttcctgc aagataataa gaaattaatg gtcttgaata attaaagtaa
     5881 tcttcatctg aaaaaactaa tatctctcct tctttatcat tttctcttat tccttctata
     5941 caacttacac cagaagcaga attcccaatt attatatacc tttttttatt tctcaattcg
     6001 ttctcctttt tcatttacaa gtattattgc ttcattcgga caattttcaa cacatgcagg
     6061 aaatccttct tcaatacata aatcacattt tgaagcaact tttttatttt ctttatcaat
     6121 ctttatcaca ccaagcggac ataccataac acacatccaa caaccaatac atttttcttt
     6181 attatgtaaa actgcttttg tattttcatt tctatacatt gctcctgtta agcatgcttc
     6241 aatacattga gcatcttcac aatgtctgca ttggattgca aaagaaatat ggcctaattc
     6301 ttcaaaatat atccctgaaa ctggttttgg ctcatgacct ttataagttt taattatatc
     6361 ctttgttttt gaatgtttcg ttatacaatg aacttcacat aatctacaac ctatacagta
     6421 ctcttctcta attattattc tttttaccat gcttcacctg cgtgttttat accaagcatt
     6481 ttaagttcaa tttcatttaa atcaatccct cttaattgtt ctctattccc tctcaatgac
     6541 tctattgcat ttatacccat gcctccaagc atttccttaa tttcaaggga ccaagccctt
     6601 aataaattat aaaccctttt tgcacctatt tctggattta atcttttcgt aagatatggg
     6661 tcctgtgttg ctattcccca attacatttt ccagtataac atttttgaca taaatgacac
     6721 ccaagagaga ttaaggcagc agttcctata taaacagcat cagcaccaag agcaatcgct
     6781 tttataacat cagaactttt tctaaaacca cctgctgcta ttattgaggc attatttctt
     6841 atgccttctt ctcttaatct ctggtcaaca accgcgattg caatttcaat aggaattcca
     6901 acattgtctc ttattatttt tggtgctgct cctgtcccac ctcttacacc atcaataact
     6961 atataatcaa caccagccct tactattcca cttgcaattg gagcaacatt atgaactgct
     7021 gcaattttaa ctccaacagg ttttttataa gaagttgcct cttttaatgc aaatataagt
     7081 tgttttaaat cctcaattga atatatgtca tgatgtggtg caggagataa agcatctgtt
     7141 ccctctggta tcattcttgt tttacttatt tgttctgtta ctttttctcc tggaagatgc
     7201 cctccaattc ctggttttgc tccttgacct attttaatct caattgcacc tgctttttca
     7261 agataatcaa tattaactcc aaatctacca cttgcaactt gaacaataca tctatccgca
     7321 tattcatata attcttcagg taaaccacct tctcctgtat tccagtaagt tccaaaatct
     7381 tttgctgcct ttgcaagtga tttcattgca tttaaagata ttgaaccaaa actcatagca
     7441 gaaaacataa tcggtgtctc aagttcaagt tgtggaggaa tttttgtttt taaaattaaa
     7501 tttccatttt taaattcata ctcaattcta tcaggttttc ttcctatgaa tgtcttcaat
     7561 tccattggtt ctcttaaagg gtctattgaa ggatttgtaa cctgggatgc attaagaagt
     7621 atatggtcaa aataattttt ataatgttta tcacatccca ttcctgtcaa aataactcct
     7681 cctgtttctg cttgcttttg aatatccaca atatattctt ttttccagtg ataattctct
     7741 tttatatcag aagtatttct tttaatttct attgcttctg ttggacatat actttcacaa
     7801 aaatggcatc ctacacattt agaattatca tttcttacaa tgtcaaactc ctcatcataa
     7861 atatgaacat cattagaaca catacgaacg catgcttgac actttataca tttttctaaa
     7921 tctctatcta caacaaattc tgatattttt tttacttttg ccattttatt ttaaatataa
     7981 ctggttttcc tgcttcaatt tcataaacat tttttaaatc tttacttata attcttattg
     8041 ctgattcttc agaagataaa taataaaagt cctcatcttc accagcaaca agtggtctca
     8101 atttaatcct atcatttaaa cctaccatat attcagaatt tgcaactatt attccaaaag
     8161 gtccattaac aaggccaggt ccatatataa tccttaaact ttttaaaaaa tttttacaat
     8221 tttcatcttc aatcctttct atttgaaacc aaaaaggaga agcaagtata tttccaattg
     8281 tttcaaaatc aattttatgt tttctataaa gcaaatcaaa aaggtaagta atgacttctg
     8341 tatcagtaaa aagagtacag taatacccaa aattttcaag atatcttcta tttataccat
     8401 aacttgatat ttcgccatta tgaacaacag cccagttaag aattccaaat ggatgtgctc
     8461 ctccccacca tccaacagta tttgtaggaa atctattatg tgaaatccat aaatatcctt
     8521 tataatcata aattttaaaa aaatcagcaa tttcatctgg atttcctaca cctttaaaaa
     8581 tacccatatt ttttccagat gaaataacaa aagcactctc tattttttta tttataaaca
     8641 taacagtttt tacaataaaa tcttcttcag ttgacttagc ttcttcatta attttttctt
     8701 cctttatctt caaaaaatac ctccacataa tagggatatt tttcaatttg tgatttttat
     8761 tcgttggaat tgattctgaa tgaattattt caaaatttgc ttccagataa tcttcacaag
     8821 tttctatatc tttttcacta ttatacataa tatggaaaca ccatttatca gaaaattcag
     8881 gataaattcc ataaccagca aatccagaac caaggccatt tcctctttca accatattac
     8941 aaattgataa aataattctt tcaccagaaa ataaattccc attttttgaa ataattccac
     9001 ttactccaca accagatggt atcttacaaa ttctatcatc catttttata gaattggcaa
     9061 atatgtttta atttcatatt catggactat ttttctatat tcatcccatt caatcttttt
     9121 tgcagtaagt aagtttgtat atatatgttc tccaagtgtt tctttaagca attggctttt
     9181 ttctgctatc tctattgctt ctattaaact tcctggtaaa acatcaagtc ccaatctttt
     9241 cctatcttcc tctgttaaat ggtatacatc catctcaagt ggttcaggta atttataatt
     9301 tccttcaatc cctttcattc ctgcctttac catacacgca aatgcaagat agggattgca
     9361 tacagggtct ggtgaacgaa attctatcct tgttgatttt tctttaccag gtctatacat
     9421 aggaactctt atcaatgtgc ttctattcct tcttgcccaa catatataaa caggtgcttc
     9481 atatccagga acaagtcgct tataagaatt tacccattga ttacatacaa atgttatctc
     9541 ttttgcatgt tttaaaagac ctgctatgta cttttttgca atatcgctta aataaaattc
     9601 atcctttggg tcaaaaaatg cattcttttc tcctttaaat aatgactgat gcgtatgcat
     9661 tccacttcca ttaactccat aaattggctt tggcataaat gtaatgtaaa atccatattt
     9721 tgaagcaatc tcctttgcta tcatcctata aaacataact atatctgcca ttgttaatgc
     9781 atctgtatat cttaaatcaa tctcatgctg acttggtgca acctcatgat gtgaatattc
     9841 aacctttatt cccatctcct caagtgtcaa aataatctcc ctccttacat cagttgctgc
     9901 atcaagtgtt gctacatcaa aataaccacc attatctaaa atttctgtca tcttatcact
     9961 tttaaatata aaaaattcaa gttcaggtcc aacataaaat gtatatcctt tctctttcat
    10021 atattctaaa tttttcttca atatatatct tgaatcacct tcataaggag ttctatctgg
    10081 atttaaaata tcgcatatca ttgctgcaac tgctttttcc tttggtctat aaggtaaaat
    10141 tgtaaatgtt tttggatcag gaagagcaac catatctgat tcatctatcc ttgcaaatcc
    10201 ctttattgaa gaaccatcaa atcccattcc atcttcaagt gctccctcaa gttcttcaac
    10261 tggaattgaa aaacccttca tctgtcctgt gatatctgaa aaaaataacc ttataaattt
    10321 aacatctttt tccttacaaa ttttcaacac atcttctttt tttaatgact tatccatctt
    10381 atccctcctt ttttaaaaaa ttttttatta ttatttctcc tgtttctttt atcttttcta
    10441 actttttata atatactttt ttaactccaa aatcaaaaag taatttttta ttaatctcta
    10501 tatgaaattg aaaaccgtaa agattttcac ctattttaaa accttgattt aaacattttt
    10561 ttcctttaac aagtaaaatt ccattttcag gaagttcaaa acaatctgaa tggtattgaa
    10621 aacatgtaaa agtttctgaa actccagtaa aaatattatc ttttttccca ttttcagtca
    10681 attcaacttc aaaaaatcct atttctttgc aaaagtttaa atttactttt gcaccaaatg
    10741 tttttgcaag aatctgtgct ccaagacaaa ttcccaaaaa atgtttttta aattgataaa
    10801 ttttttttat aagatcttct tcctctttta aaaaaggata ttctctctcc tgataaacat
    10861 tcataggacc tccaagagaa aaaataaaat caatattctt aatatcttct ggtaattttt
    10921 ctccattttc taactcaata attttatata atatacccat ctcttttaaa atatcttcaa
    10981 ttattccagg accttcttca attttatgct taattattaa acatttttta aattgcttca
    11041 tcaccttttt ctcctgtcct tattcttatt gcatcttcta tcggtatcac aataaattta
    11101 attccaagat tttctgctaa tttttttgca tcattaaaag attgagaaga ggaaaacctt
    11161 gaaggcataa aaacacctat aacattatct tttccaagag catcagtagc aattgttgcc
    11221 acaagggatg aatcaatacc accacttaaa cctataacaa cttttttaaa attattttta
    11281 taaacatagt cctttaatcc acaaataagt gctttatata tctcctcttc ttcactaatt
    11341 tctataattt tctcctgttt ttcatttgtt gaaaaaaata aaatctcttc ttcaaaggga
    11401 cttgctttct ttataatttc tccttttgga ttcattgcaa aactttgacc atcaaaaaca
    11461 agttcatcct gaccaccaac aagatttgta taaaataaaa aaacattatt ttcagtgcat
    11521 atatttctta ctaaattttc tctttcttta atttttctga aatgataagg ggaagcattt
    11581 aaagtgaaaa tatattttgc tccatctttt atctgttttg cataaggatc atttttatgc
    11641 catatatctt cacatattgt aataccaaat ttaattccat taatttcata aataccctct
    11701 ttttttcctt catcaaaata tctcttttca tcaaaaacat cataatttgg aagaattttt
    11761 ttattgtaaa tcccaattat ttttctttta taaattatcg cagcagagtt gtaaatcttt
    11821 gaatcttctc tatttacaaa accaactatt acaaccatat cctctacttt attttttatt
    11881 ctttcaactg cttttaagtt atcttggata aatttatttt tcaaaagtaa gtcctctggt
    11941 ggatatcctg taattgaaag ttctggaaaa caaagaaaat tacattttaa ttcttttgcc
    12001 ttttcaattg ttcttattat tttttcagta ttaccattta tatctccaac aataaaattt
    12061 aattgacata aacctataaa aagattatcc ataaaaacct cctttttatt attttcccaa
    12121 cactggaaag gacttctttg tctggtggga gcacatcctc catgctaaat tttatattaa
    12181 aaaattttaa agtcaaattt ttctctcttt tgggttaaga catagtatcc aggtagacaa
    12241 gaaatggaaa agagatatat ggagaaggag agatttagag gtaattgttc caaaggataa
    12301 aagacaggcg aaaaaattag ttaaggagat ataaagagaa ggaaggatta aaggacttat
    12361 caagaaggcc agagaattca ccgaacaaaa cactaccata tttagaaagg ataataatag
    12421 cagaaagaaa aaagattcat tctataatag aaatgacacc ttatgagaaa ctttgtaaag
    12481 attataaatt accagatgag atttgtttat ttcctgttat taatcttgat gaaatacctg
    12541 atatcttaaa ttcttatttc aaaaatttct accttaaccc taaatacaat ctcttaaccc
    12601 agtcctattt ttaattactg aatggaattg attttttatg ataaaatatt aaaaagtttt
    12661 gaaagtaatg aaaaaataag ataatataaa aaaatgaaga aatttatttt tattaccggt
    12721 ggagttgtat cttctctagg aaagggtatt gcttctgctt caatagggac cttacttgaa
    12781 tcacatggat ataaggttac ttttttgaaa tttgacccat atataaatgt tgaccccgga
    12841 acaatgagtc catttcagca tggagaggta tatgtaactg aagatggtac agaaacagac
    12901 cttgaccttg gccattatga aagatttaca aatgcaaaga tgactcata
//
LOCUS       DAOSAT010000051        12686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3332_length_12686_cov_5.189138, whole genome shotgun sequence.
ACCESSION   DAOSAT010000051 DAOSAT010000000
VERSION     DAOSAT010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12686
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3332_length_12686_cov_5.189138"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PLF90_05640"
     CDS             complement(<1..879)
                     /locus_tag="PLF90_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOK56794.1"
                     /translation="MVEIKKIIEESLNEAKEFLNDLIRFESISGIGEEKIQNFIYEKF
                     KKFGKSSLVKIDENIKKDPEYTCSDKPCDYSKRKNLILEIGKGEGYTLILNTHSDIVP
                     ATNWKNAFIPEEKEGYIYGRGACDAKGQIATIYLTLLTLKKMNIEISGKLIIEIVVEE
                     EIGGNGTLSLIRQGYKGDGVIVLEPTELKITPANRGAVWFKLELFGKSVHMGKIWEGV
                     NAIEKFCYLYPKLKDFEKKLIEESKNVPLFERFKQPVQLNFGVIKSDGWPSMVCGKVE
                     IEGGIGFLPNKTLDGIK"
     gene            complement(872..2227)
                     /locus_tag="PLF90_05645"
     CDS             complement(872..2227)
                     /locus_tag="PLF90_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOK56795.1"
                     /translation="MGKEFELIPKKVKKIETEFRRIVTDIPVPESIPVLEKLRKYEPR
                     SMSGQPPIVWDRAKGINVFDKYGNMWLDFSSGVLVANAGHGNDFVLDEIKKQIEKPLL
                     HNYCFPSEIRAELVEKIASLSPEPLKKVFLLTTGAESVECAIKLSRTYGKKKNKKVII
                     SFEGAFHGRTLGAQMIGGIPNLKEWIVNPDHDIIQVPFPGDPRCKDKSFDLFLRTLKE
                     KGVEEKDVCAVISETYQGGNASFMPVEYAKNLREWCDKNEVLLIFDEIQAGFGRTGTF
                     WGFQHYGVIPDMFILGKGISSSLPISAVVGKKEIMDQYEPGTMTSTHTGNPLCCAAAF
                     GSIKYIEKYKLWENAEKMGKIFERELLKLKKYDCVGFICGKGLVWGLHIVKAKTEEPE
                     PDIAFKIVEKCFEKGLLFFAPVGFKGATIKVNPPLIINEKAVLEGCEVIEDAIMEVFG
                     G"
     gene            complement(2323..2877)
                     /locus_tag="PLF90_05650"
     CDS             complement(2323..2877)
                     /locus_tag="PLF90_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOK56796.1"
                     /translation="MAEKLKRKNIVVGVTGSIAIYKSCEVVRNLIKNNWSVKVVMTEN
                     AKKFVSPLTFETLSRNPVYSDMFEKKTYEEDHIALADFASIILVCPATANIIGKVSSG
                     ICDDLLTATIFAFSGPVIFAPAMNENMWLNSIVQENVKKLKKHGYYFVEPEEGELASG
                     KTGKGRLAKIEKIIDFVEKVYNKH"
     gene            2958..3719
                     /locus_tag="PLF90_05655"
     CDS             2958..3719
                     /locus_tag="PLF90_05655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOK56797.1"
                     /translation="MEEYRLKLKGEIIEDYKIEEMIWHGGTSNIYKGVSLNSNFHKIV
                     AIKVLHPHRNFSHQIKSFEKEFKILKKLSHPNILKVYKFGKIDKLYYIIMEYIDGMSL
                     KIMLNEKAEVSPNTLLKILIKVGETIEYIHSKKIVHNDIKPENILISKNFLDLKLIDF
                     GYAEKLSFFRKRVNYTGGTEKYIAPERKNGIIDFKSDIYSYGVMLEEILSGYDLIEEI
                     YPVILSAKSENPEKRPHLKDIIKKLKAIYENWNNQ"
     gene            3697..4431
                     /locus_tag="PLF90_05660"
     CDS             3697..4431
                     /locus_tag="PLF90_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOK56798.1"
                     /translation="MKIGIISDIHSNLEAFQSTINFYEKNKVDKIIICGDIIGYGPDP
                     NECIDLAEKNAEVILKGNHEEGIIKNDFSKFKKFAKITLEWTIKEIKEKIEKILKWKE
                     KEEFGDLLFFHASITDNYYKYLFKITDTEEEFEKLNRKICFIGHTHIPVVFRKDMNTG
                     KIEKILTDFSGRIELDIEENFKYIINTGSVGFPRDGFPFACVSVYDTDRKNFKLYRIE
                     YEIEKTIKKILEKGLPSKICNYLKGF"
     gene            complement(4417..4872)
                     /locus_tag="PLF90_05665"
     CDS             complement(4417..4872)
                     /locus_tag="PLF90_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56799.1"
                     /translation="MKKLILVVFAFSVLTGFSEIKKATYEEIKNSIQILENAMKEEKE
                     DLIPIFSEAIEIEKRATSPYYAEKIMEKICKTGKIKEEEFKNLRKNFSFFDISLAWAI
                     NQITGKPVEKILKEKEENGWINIISSETTIQREDISSKIKELNPKSETF"
     gene            complement(4910..5383)
                     /locus_tag="PLF90_05670"
     CDS             complement(4910..5383)
                     /locus_tag="PLF90_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56800.1"
                     /translation="MRKRKIFITFIFVALPFFCIFIFHFSVPFIWLKVDQAFHCGDYN
                     RAIEYLSFISYMQPKNPECYILKAWLQWSEAKNCYNRGIPYEEKLNQAIETYRKGQKN
                     NPTDWKIYFEEGIMWEAFGEKEKALNSYYISSKYSRPPYNKIYKIKSEKFKIEVK"
     gene            complement(5390..5463)
                     /locus_tag="PLF90_05675"
     tRNA            complement(5390..5463)
                     /locus_tag="PLF90_05675"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5427..5429),aa:Arg,seq:ccg)
     gene            complement(5573..6196)
                     /locus_tag="PLF90_05680"
     CDS             complement(5573..6196)
                     /locus_tag="PLF90_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK56801.1"
                     /translation="MKKPKIRELKEAITSLISKPYTTEFPYKEHKPFPRFRGKPKYNE
                     EKCVGCGGCYQVCPSKAIDMIDDIENKKRILIHNPDRCIFCGECERNCITKEGIKLTN
                     QFDVSYLKDVNEVQNKIEHDLVICVTCGEIIGTEKHIRHIYSKLGNLAFSQPVIITEL
                     IKKLKIPTEYEEKFVPPINRQDVLKVVCPRCRRIAFIKDEVKGPVKK"
     gene            complement(6193..6513)
                     /gene="mnhG"
                     /locus_tag="PLF90_05685"
     CDS             complement(6193..6513)
                     /gene="mnhG"
                     /locus_tag="PLF90_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOK56802.1"
                     /translation="MREIISLIFMFIGGLFNLFGCIGLVRFPDIYNRLQASTKCVTLG
                     TCMILLGVIVSGPLDISLKGFLCAIFILITGPTAAHALSNASIKAGIKLWEKSVVNKM
                     EEKE"
     gene            complement(6522..6779)
                     /locus_tag="PLF90_05690"
     CDS             complement(6522..6779)
                     /locus_tag="PLF90_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOK56803.1"
                     /translation="MITLLITLLLFCCFMCLYRISRGPTPADRVVGIDILGIIVVNFC
                     AIVSFMTKIDFYMNIALSWALLSFIGTLAVSKFLSGKGFDE"
     gene            complement(6776..7276)
                     /locus_tag="PLF90_05695"
     CDS             complement(6776..7276)
                     /locus_tag="PLF90_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOK56804.1"
                     /translation="MKKGILGILLFIAWIFLTWPFKYGIDWQVILAGILISILVGMIF
                     GEGFTESPHKFFDIKRYLWAIGYIPVLIFWCIVANFDVAYRVLHPNLPIRPGIVKVKT
                     RLKNKSALTLLANSITLTPGTMSVEIDPDGFLYIHWINVKTDDIEKATEIIVRKFEKI
                     IERIFE"
     gene            complement(7278..8765)
                     /locus_tag="PLF90_05700"
     CDS             complement(7278..8765)
                     /locus_tag="PLF90_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOK56805.1"
                     /translation="MVNLPLFIVIPFGVAFFIPMVSRKIKWVPDVLGNLTTFILLLLS
                     LMTLNQEYVYHIGGWKAPFGIVLVLDGLSCLLLIIINLISFMATLFSINYIEKRFTSK
                     LRYYSLFLLMVGGMNGTVLSGDLFNLFVFMEIASIASYALVGFGCQASELEASFKYLV
                     MGTVSGLFVLYGVALLYGKTGTLNIADVARILRDIPYDNLIGFAFVLIISGFMIKSAL
                     VPFHAWLPDAHPSAPAPISSMLSGVVIKALGVYVICRLIFNLFGFTPLLSQILVSLGV
                     ISMIVGVCLALLQWDLKRLLAYHSISQIGYVFVGIGLGTPLGILGGIFHLFNHSLFKS
                     LLFLDSGSIEYETGTRDLNKMGLLYKKMPITSFSTLVASLSISGIPPFNGFWSKLIII
                     LAAIKAGRPVIALWAVIGSILTLSSFTKVLRYAIYGKGEKFDKSKLEKIKEVPDTMIV
                     PLIILSILCIFTGLLLIGMTDGIFGPVIDVFDGGVLQYISKVLGV"
     gene            complement(8770..9117)
                     /locus_tag="PLF90_05705"
     CDS             complement(8770..9117)
                     /locus_tag="PLF90_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOK56806.1"
                     /translation="MIYYLCFVLFLIGLYGVCCKKNVVKIILGLWVMEYSVNLFLILI
                     GYRKNGIPPILQPNTNIVEFSQKAVDPLPQAMVLTSIVIGLGILALMVSIAIRLYQRY
                     GTFDITEMRKLKG"
     gene            complement(9114..9611)
                     /locus_tag="PLF90_05710"
     CDS             complement(9114..9611)
                     /locus_tag="PLF90_05710"
                     /inference="COORDINATES: protein motif:HMM:NF006248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOK56807.1"
                     /translation="MNNGEKGMSLIVKTVTRWIKGFIFLFGAYIVVFGHLSPGGGFPG
                     GVVFAATFILITLAFGREYALRKIGKVLASELDSLGAILFLLIATLGIYFTGVFFSNF
                     IEKYHPTGNFKLFSAGIIPLCNLSIGLKVGACLFMIFIILSVVRVIFEDGKGKLITTE
                     KRDKK"
     gene            complement(9604..10155)
                     /locus_tag="PLF90_05715"
     CDS             complement(9604..10155)
                     /locus_tag="PLF90_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOK56808.1"
                     /translation="MVEIIVGILLVFMIIGSIICLETKDLLSAIIAIVPVGYFSGIIF
                     LLLGAPDIAITQMVVEVLSLIILIRATIRRDLTTIEEERDFFGLITTFAFIFVFFIFA
                     MEALRYLPSFGTPSFTLNPDAPSNRYLLNGLKETGAGNIVNGIIFDYRGYDTLGEATI
                     LFTSILGALAILRVYARKRSKDE"
     gene            complement(10165..11961)
                     /locus_tag="PLF90_05720"
     CDS             complement(10165..11961)
                     /locus_tag="PLF90_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOK56809.1"
                     /translation="MEKIVLSIFSVGFISGILCRIIPSRIKYLKEIISGLVSLLFLSF
                     SILLFKGSPYYITEIFLIDPLSIFISIFISLFTLLVLIYSFSYLENFENSNRYYAYIL
                     WTLSSSIGAIFSNNLVLFSVFWGFLAITLYLILSIFGQENAYSAKKTMIIVGGSDSFL
                     IFGICGLIYLTGKYTITDMNIFIASGLEIGIFFSLICASFAKAGCMPFHTWIPEIAEN
                     TPSPILAYLPASLDKLLGIYLFSRILLNIFEYSNDLHSGLWFILRLIGSLTIVCAVLM
                     AIVQHNMKKLLSYHAISQVGYMVLGIASTNPAGIIGGLFHMLNNSIYKSLLFFGAGNV
                     EKKTKEVELEKLGGLSRFMPITFITFLIGSLSISGVPPFNGFFSKYLIYQGLFEGAKT
                     GDFSWNIWIICGMIGSALTLASFLKIVHSVFLGHIKEYKEKIEEVGFLMLLPVIVLSL
                     LCILFGFGFLYPVKIFENSIGIFIEKGYDYLLPTLTLIFISLIFGLIIFLIFLPSRKR
                     TVSTFIGGEKKLKEMEMSGTEFYKTIEKQPFLTKMYEGAKKKIFDLYEDGKRIVFYLG
                     EGIRATHTGVLPTYLTWILIGCIVLFVLFIKF"
     gene            complement(11943..>12686)
                     /locus_tag="PLF90_05725"
     CDS             complement(11943..>12686)
                     /locus_tag="PLF90_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HOK56810.1"
                     /translation="IEDSDIPILKKTLSDVKGKIQMLTEAVLDDPVLGARLKGVGVLT
                     KEDIKNYGAVGPVARASGVDIDVRKDDPYAAYNLVEWKVITTNNGDVFDKAVVRLLEC
                     FESIKIIEQSLDAIYGKKGQIWKEVREIPKGEGIGHHEAPRGEVFHYIRSDGNTSSPV
                     RHKIRAPSYVNIPTFKASCIGETISDVCIILAGVDPCYCCTERIAVYDKEKNKKIYSF
                     EDLVKKSIEKTEKIRKEIGKEEKLWKKLF"
BASE COUNT         4613 a         2263 c         1483 g         4327 t
ORIGIN      
        1 ttttatccca tcaagtgtct tattcggtaa aaatccaatt ccaccttcaa tttcaacttt
       61 tccacagacc atacttggcc atccatcact tttaatcact ccaaaattca attgaaccgg
      121 ctgtttaaat ctttcaaata gagggacatt tttactttcc tcaatcaatt ttttttcaaa
      181 atctttaagt ttcggataaa gataacagaa cttttcaatt gcattaacac cttcccatat
      241 tttacccata tggacactct tcccaaacaa ttcaagttta aaccatacag cacctctatt
      301 tgcaggagta attttaagtt ctgtcggctc aaggacaata acgccatccc ctttatatcc
      361 ctgccttatt aaagacaaag tcccattccc accaatttcc tcttcaacaa caatttcaat
      421 tataagtttc cctgaaattt ctatattcat ttttttaagt gtaagtaagg ttaaataaat
      481 tgttgcaatc tgacctttcg catcacaagc accccttccg taaatataac cctccttctc
      541 ttctggaata aaagcattct tccagttggt tgcaggtact atatctgaat gagtatttaa
      601 aattagagta tatccttcac cttttcctat ttcaagaata agattttttc tttttgagta
      661 atcacatggc ttgtcactgc atgtgtattc agggtctttt tttatatttt catctatttt
      721 aacaagagag cttttaccga attttttaaa tttttcataa ataaaatttt ggattttttc
      781 ttcacctata cctgaaatac tctcaaatct tatcaagtca tttaaaaatt cttttgcctc
      841 atttaaactt tcttcaatta tttttttaat ttcaaccacc aaaaacctcc attattgcat
      901 cttcaataac ttcacatcct tctaaaaccg ctttttcatt tataataaga ggtggattta
      961 cttttatagt cgcaccctta aaaccaactg gagcaaaaaa taacaaacct ttttcaaaac
     1021 atttttcaac gattttaaat gcaatatcag gttctggttc ttcggttttt gcctttacta
     1081 tatgtaatcc ccatacaagc ccttttccac agataaaacc aacgcaatca tattttttca
     1141 attttaaaag ttctctttca aaaattttcc ccattttttc ggcattctcc cacaatttat
     1201 atttttctat atatttaata cttccaaaag cagcagcaca gcacaaagga tttcctgtat
     1261 gggtacttgt cattgtcccc ggttcatact ggtccataat ttcttttttt ccaacaaccg
     1321 cagatattgg aagagaactt gaaatcccct ttccaagaat aaacatatca ggaattacac
     1381 cataatgctg aaatccccag aaagtccctg tccttccaaa acctgcctgt atttcatcaa
     1441 atattaaaag aacttcattt ttatcacacc attcccttaa atttttagca tattcaacag
     1501 gcataaaaga tgcatttcca ccctgatatg tttcacttat aacagcacaa acatcttttt
     1561 cttccactcc tttttccttt aatgtcctta aaaataaatc aaaactttta tccttacatc
     1621 tcgggtctcc tggaaaagga acctgtataa tatcatggtc aggattaaca atccattctt
     1681 ttagatttgg tattcctcct atcatctgag ctccgagtgt tcttccatga aaagcacctt
     1741 caaaggagat tataactttt ttatttttct tttttccata agttcttgaa agttttatcg
     1801 cacattcaac actttcagca cctgttgtaa gaagaaaaac cttttttaaa ggttcgggag
     1861 aaagagaagc aattttttca acaagttctg ctcttatttc agaaggaaaa cagtaattat
     1921 gtaaaagtgg tttttcaatc tgttttttta tctcatcaag aacaaaatca ttcccatgac
     1981 ctgcatttgc aacaagaacg ccagaagaaa aatccagcca catatttcca tacttatcaa
     2041 acacatttat accctttgcc ctatcccata ctattggtgg ttgacctgac attgaacggg
     2101 gttcatactt tctcaatttt tcaagaacag gtattgattc aggaacaggt atatctgtaa
     2161 caattctcct gaattctgtt tcaatttttt ttaccttttt aggaattaat tcaaattctt
     2221 ttcccacttt tacctcctct tttcatttta ttaaaaaaat caagtatata atactacttt
     2281 ttattttaag attcaaaaca gagtaaaaat tctgacaaaa aattaatgct tattatatac
     2341 tttctcaaca aaatctatta ttttttctat ttttgcaagc cgaccctttc cagtttttcc
     2401 acttgcaagt tcaccctctt ccggttctac aaaataatat ccatgctttt tcaatttttt
     2461 aacattttcc tgtactattg aattcaacca catattttca ttcattgcag gagcaaaaat
     2521 aacaggtcca ctaaaagcaa aaattgtagc agttaaaagg tcatcacata ttcctgaaga
     2581 tacttttcca attatatttg cagtagcggg acatactaga attattgaag caaaatcagc
     2641 aagggcaata tggtcttcct cataagtctt tttttcaaac atatcagaat aaacaggatt
     2701 tcttgatagt gtctcaaaag tcaaaggact aacaaatttt tttgcatttt cagtcattac
     2761 tactttaaca gaccagttat tttttattaa atttctcaca acctcacaac ttttatatat
     2821 cgcgatagaa cccgtcactc caacaactat attttttctt ttcaattttt cagccataaa
     2881 gatattatac aaccatttgc aaaaaattaa aaattgtatt aaaattattt ggttttttta
     2941 tttaaaaaag aaaaactatg gaagaataca gattaaaatt aaaaggagaa attatagaag
     3001 attataaaat agaagaaatg atatggcacg gaggaacatc aaatatttat aaaggagtat
     3061 ctctgaattc aaattttcac aaaatcgttg caattaaagt cctccatcct cacagaaatt
     3121 tctcacatca aataaaatcc tttgaaaagg aattcaaaat tttaaaaaaa ctctcacatc
     3181 caaatatttt aaaagtttac aaatttggta aaattgataa actatattat ataattatgg
     3241 aatacattga tggaatgagt ttaaaaatca tgttaaatga aaaagcagaa gtttccccta
     3301 acacactttt aaaaatatta ataaaagtcg gagaaacgat tgaatatata cactcaaaaa
     3361 agattgttca caatgatata aaaccagaaa atattcttat cagtaaaaac ttccttgatt
     3421 taaaacttat tgattttggt tatgcagaaa aactatcatt tttcagaaaa agagttaact
     3481 atacaggagg gactgaaaaa tatattgctc ctgaaagaaa aaatggtatt attgatttta
     3541 aaagcgatat ttattcttat ggagtaatgc tggaagaaat tttatccggt tatgatttaa
     3601 ttgaagaaat ttatccggtt atactctcag caaaatctga aaatcctgaa aaaagacctc
     3661 atttaaaaga cataataaaa aaattaaagg caatttatga aaattggaat aatcagtgat
     3721 atacattcaa accttgaagc atttcaatcc accattaact tttatgaaaa aaataaagtt
     3781 gataaaatta ttatctgtgg agatattatt ggatatggtc ctgacccgaa tgaatgtata
     3841 gatttagcag aaaaaaatgc agaagttata ttaaaaggaa atcatgaaga aggaattata
     3901 aaaaatgatt tttcaaaatt taaaaaattt gcaaaaatta cacttgaatg gacaattaaa
     3961 gaaattaaag aaaaaataga aaaaatttta aagtggaaag aaaaagaaga atttggtgat
     4021 ttactttttt ttcatgcttc tataactgat aattactaca aatatctttt taaaataaca
     4081 gatacagaag aagaatttga aaaacttaat agaaaaatat gtttcatagg acatactcat
     4141 attccagtag ttttcagaaa agatatgaat acagggaaaa ttgaaaaaat tttaactgat
     4201 ttttctggaa ggattgaact tgatatagaa gaaaatttta aatacataat aaatacagga
     4261 agtgttggtt ttccaagaga tggtttccca tttgcttgtg tttctgttta tgatactgat
     4321 agaaaaaatt ttaaacttta cagaattgaa tatgaaattg aaaaaactat taaaaaaata
     4381 ttggaaaaag gattgccctc aaaaatctgt aattatttaa aaggtttctg atttaggatt
     4441 caattctttt atttttgatg aaatatcttc cctttggatt gtagtttcag aagaaataat
     4501 atttatccag ccattttctt ctttctcttt taatatcttt tcaacaggtt ttcctgtaat
     4561 ttgatttatt gcccaggcaa gtgatatatc aaaaaatgaa aagttttttc ttaaattttt
     4621 aaattcttct tcctttattt ttcctgtctt acaaattttc tccattattt tttctgcata
     4681 ataaggagaa gttgctcttt tttctatttc aattgcttca ctgaatatag gaattaaatc
     4741 ttctttttct tctttcatcg cattttcaag tatctgaatt gaatttttta tttcttcata
     4801 agtggctttt ttaatttctg aaaatccagt aagaactgaa aatgcaaaaa caactaaaat
     4861 taactttttc ataaaaccct cctttttttc aatccttttt accctattat tattttacct
     4921 caattttaaa tttttcactt ttaattttat aaattttatt ataaggagga cgactgtatt
     4981 ttgaacttat ataatatgaa ttaagtgctt tttctttttc tccaaatgct tcccacataa
     5041 ttccttcttc aaaataaatc ttccagtcag tcgggttatt cttctgtcct tttctgtatg
     5101 tttctattgc ttgattcaat ttctcttcat aaggaatacc tctattataa caattttttg
     5161 cttctgacca ctgtaaccat gcttttaaaa tataacattc aggatttttt ggctgcatat
     5221 aagaaatgaa tgataaatat tcaattgctc tattataatc tccacaatga aatgcctggt
     5281 caactttaag ccagataaat ggaactgaaa aatgaaatat aaaaatacag aaaaatggaa
     5341 gtgctacaaa aataaaagtt ataaaaattt ttctttttct catatccttt gcgcttgccg
     5401 ggaattgaac ccggattctc tggctccgga ggccagcgct ttatccactt aagccacaag
     5461 cgccatatta aaattttaaa ccatgcatat aaattttcaa gtttaaaagt caaaaatata
     5521 ttaattacct tttggacacc tacgaggtgg ctggtgggaa ttgttgaagg acttactttt
     5581 tgacagggcc ttttacttca tcttttataa aggcaattct tctacatctc ggacaaacta
     5641 cttttaaaac atcctgtcta tttataggag gtacaaattt ttcttcatat tcagttggta
     5701 tttttaactt tttaatcaac tctgttatta tgactggctg gctgaatgca agattaccaa
     5761 gtttactgta aatatgcctt atatgttttt cagttcctat aatttctcca caggtgacac
     5821 agatgactaa atcatgctca attttattct gaacctcatt tacatctttt aaatatgaaa
     5881 catcaaattg atttgtaagt tttatacctt cctttgttat acaatttctc tcacattctc
     5941 cacagaaaat acatctatca ggattatgaa ttaaaattct ttttttattt tcaatatcat
     6001 ctatcatatc aattgctttt gaaggacata cttgatagca acctccacat ccaacacatt
     6061 tttcctcatt atattttggt tttcccctaa atcttggaaa tggtttatgc tctttgtatg
     6121 gaaattctgt tgtatatggc tttgaaatta aacttgttat tgcctctttt aattccctta
     6181 tttttggttt tttcattcct tttcctccat tttattcaca acacttttct cccataattt
     6241 tattcctgcc tttatactag catttgataa agcatgagca gcagttggtc cagttattaa
     6301 aataaagata gcacacaaaa atccttttaa agaaatatct aaaggaccac ttacaataac
     6361 accaagtaaa atcatacatg ttccaagagt tacacatttt gttgatgctt gtaatctatt
     6421 ataaatatca ggaaaacgaa caagtcctat gcaaccaaaa agattaaata accctcctat
     6481 aaacataaaa attaaactta taatttctct cattttttac ctcactcatc aaatccttta
     6541 ccacttaaaa atttacttac agcaagtgtt cctataaaac tcaataaagc ccatgataaa
     6601 gcaatattca tataaaaatc aatctttgtc ataaatgaaa ctattgcgca gaagtttaca
     6661 acaattatac caagaatatc aattccaaca accctatcag caggagttgg accccttgag
     6721 attctataaa gacacataaa acaacagaat aaaagcaaag taatcaaaag agtaatcatt
     6781 caaatatcct ttctattatt ttttcaaatt ttcttacaat aatttcagtt gccttttcaa
     6841 tatcatctgt ttttacattt atccagtgaa tatataaaaa accatcaggg tcaatttcaa
     6901 cactcatcgt tcctggtgtt aaagttattg aatttgcaag aagtgttaaa gcacttttat
     6961 ttttcaatct tgtttttact ttaacaattc ctggtcttat tggtaaattt ggatgtagaa
     7021 ccctataagc aacatcaaaa tttgcaacaa tacaccagaa tattaaaaca ggaatataac
     7081 caattgccca taaatacctt tttatatcaa agaacttatg tggactttca gtaaatccct
     7141 ctccaaaaat cataccaacc aaaatagaga ttaaaattcc tgctaaaata acctgccaat
     7201 caatcccata tttaaaaggc catgttaaaa atatccatgc aataaaaagt aaaattccta
     7261 atataccctt tttcatatta tactcccagt actttactta tatattgtaa aacacctcca
     7321 tcaaagacat caataacagg accaaaaatt ccatctgtca ttcctattaa aagaagtcct
     7381 gtaaaaatac ataaaattga aagaattatc aaaggaacaa tcatggtatc aggaacctct
     7441 tttatttttt caagtttact tttgtcaaat ttttctccct ttccataaat tgcatatctt
     7501 aaaacttttg taaatgaaga taatgttaaa atacttccta ttacagccca taaagcaatt
     7561 acaggtctac ctgcttttat tgctgcaagg ataattataa gtttactcca aaaaccatta
     7621 aaaggaggga ttcctgaaat tgagagagaa gcaacaagtg tagaaaaaga agttataggc
     7681 atttttttat aaagaagtcc cattttattt aaatcccttg ttcctgtttc atactctatg
     7741 cttcctgaat caagaaataa aagtgattta aataaagaat ggttaaataa atggaaaatc
     7801 cctccaagaa ttccaagtgg tgttccgagt ccaattccaa caaatacata accaatctgg
     7861 cttattgaat ggtatgccaa tagtcgcttt aaatcccatt gtaataaagc aagacataca
     7921 ccaacaatca ttgaaataac accaagagaa actaagatct gacttaataa aggagtaaat
     7981 ccaaaaagat taaatataag ccggcagatt acataaacac cgagtgcctt aataactaca
     8041 ccagatagca ttgaacttat gggagcagga gcagaaggat gtgcatctgg aagccaagca
     8101 tgaaaaggaa caagtgctga cttaatcata aatccagaaa ttattaaaac aaaggcaaat
     8161 ccaattaaat tatcataagg tatatctctt aaaattcttg caacatcagc aatatttaat
     8221 gtacctgtct ttccatataa aagtgcaact ccatataaaa caaataaacc agaaactgtt
     8281 cccattacaa gatatttaaa acttgcctca agttcacttg cttgacatcc gaaaccaaca
     8341 agggcatatg aggcaataga ggcaatctcc ataaatacaa ataaattaaa taggtctcca
     8401 gaaagaaccg ttccattcat tccaccaacc ataagaagaa acaaagaata atatcttaat
     8461 tttgaagtga accttttttc aatatagtta attgaaaata aagtagccat aaaagagatt
     8521 aagtttatga ttataagaag caaacaggaa agtccatcaa gaacaagaac tattccgaaa
     8581 ggtgctttcc atcctcctat atgataaaca tattcctgat taagtgtcat taaagataaa
     8641 agtaataaaa taaatgttgt taaattacca aggacatctg gaacccactt aatcttcctt
     8701 gaaaccatag ggataaagaa tgcaactcca aaaggtataa ctataaataa aggtaaattc
     8761 accattttcc tatcctttta attttctcat ttctgttata tcaaaagttc catacctttg
     8821 gtataatcta attgcaatag aaaccattaa agcaagaata ccaagaccta tgactatact
     8881 tgttaaaacc attgcctgtg gtaatgggtc aactgctttt tgtgaaaact caactatatt
     8941 tgtattaggt tgcaaaattg gtggtattcc attttttcta taaccaataa ggattaaaaa
     9001 tagattaacc gaatattcca taacccatag accaaggata atttttacaa cattcttttt
     9061 acaacagaca ccataaagac ctattaaaaa caaaacaaag cataaatagt aaatcatttt
     9121 ttatcccttt tttcagttgt tataagtttt cctttaccat cttcaaaaat aacccttaca
     9181 accgaaagaa ttataaatat cataaacaaa catgcaccaa cctttaatcc tatgcttaaa
     9241 ttacataaag gtataatccc tgcagaaaaa agtttgaaat ttcctgtagg atgatatttt
     9301 tctatgaagt ttgaaaagaa tacacctgta aaataaatac ctaaagttgc tatcaataaa
     9361 aacaaaattg cacccaaact atcaagttca gaagcaagaa ctttaccaat ttttcttaaa
     9421 gcatattctc ttccaaaagc aagtgtaatc aatataaatg ttgcagcaaa aacaacacca
     9481 ccgggaaaac caccaccagg acttaaatga ccaaaaacaa caatatatgc accaaataaa
     9541 aatataaaac ccttaatcca tcttgtaact gtcttaacta ttaaactcat tcctttttct
     9601 ccattattca tccttactcc tttttcttgc atataccctt aaaattgcaa gtgctccaag
     9661 tattgaagta aataaaattg ttgcttcacc aagtgtatca tatcccctgt aatcaaaaat
     9721 tataccattt acaatattac ccgctcctgt ttctttaagt ccatttaaaa gatatctatt
     9781 tgaaggtgca tcagggttta aagtaaaaga aggtgtccca aaggaaggca aatatcttaa
     9841 tgcttccata gcaaatataa agaaaacaaa tataaaagca aaagttgtta taagtccaaa
     9901 gaaatccctt tcttcctcaa ttgttgttaa atcccttctg atggttgctc ttattaaaat
     9961 tattaatgat aaaacctcaa caaccatttg tgttattgct atatcaggtg ctccaagtaa
    10021 aagaaatata atacctgaaa aataaccaac cggaacaatt gctataattg cagaaagtaa
    10081 atcttttgtt tcaagacata taatacttcc aattatcata aaaacaagca aaattccaac
    10141 aataatttct accatataaa ctccttaaaa tttaataaat aatacaaata aaacaataca
    10201 accaattaaa atccatgtca aataagttgg caaaacacct gtatgtgttg ctcttattcc
    10261 ttctccaaga taaaatacaa tcctttttcc atcttcatat aagtcaaata tttttttctt
    10321 tgcaccttca tacatctttg ttaagaatgg ttgcttttca attgttttat aaaattctgt
    10381 tccactcatc tccatttctt tcaatttttt ctctccacca ataaatgttg aaacagttct
    10441 tttccttgaa ggtaaaaata ttaaaaagat tattaagcca aaaattaaag aaataaaaat
    10501 taaagttaaa gttggcaata aataatcata acctttctca atgaatatac ctattgaatt
    10561 ttcaaaaatt tttacaggat agagaaatcc aaaaccaaat aaaatacata gtaaagaaag
    10621 aacaattaca ggtaataaca ttagaaatcc tacctcttca attttttctt tatattcttt
    10681 tatatgtcca agaaatacag aatggacaat ctttaagaaa cttgcaagag ttagtgcaga
    10741 acctatcata ccacagataa tccaaatatt ccaagagaaa tcccctgtct ttgctccttc
    10801 aaaaagtcct tgatagatta aatatttaga gaagaaacca ttaaaaggag gaacaccact
    10861 tattgataat gaaccaatta aaaatgttat aaatgtaatt ggcataaacc tacttaatcc
    10921 accgagtttt tcaagttcaa cttcctttgt tttcttttca acattacctg caccaaaaaa
    10981 caaaagtgat ttataaattg aattatttag catgtggaaa agacctccaa taattcctgc
    11041 tggatttgtt gatgcaattc caaggaccat atagccaacc tgacttatag catgataaga
    11101 taaaagtttt ttcatattat gttgaactat tgccattaaa acagcacaca caattgttaa
    11161 agagccaatc aatcttaaaa taaaccataa accagaatgt aaatcattac tatattcaaa
    11221 aatattaagt aaaatccttg aaaaaagata aataccaagg agtttatcaa gtgaagcagg
    11281 taaatatgca aggattggag atggtgtatt ttctgctatt tcaggaatcc atgtatgaaa
    11341 aggcatacag cctgcctttg caaatgaagc acaaattaaa gaaaagaaaa taccaatttc
    11401 aagtccactt gctataaaaa tattcatatc tgttattgta tattttcctg taagataaat
    11461 taaaccacat ataccaaaaa tcaaaaatga atcactacca ccaactatta tcattgtctt
    11521 ttttgcagaa tatgcatttt cttgtccaaa tatactcaaa attaaataaa gtgtaattgc
    11581 aagaaatccc caaaaaacag aaaaaaggac taaattattt gaaaagatag cacctattga
    11641 ggaagaaagt gtccataaaa tataagcata atatctattt gagttttcaa aattttcaag
    11701 ataagaaaaa ctataaataa ggacaagtaa tgtaaagaga gagataaaaa tagagatgaa
    11761 aatagagaga gggtctatta aaaatatttc tgtgatataa taaggagacc ctttaaaaag
    11821 caaaatagaa aaagaaagga atagtaaaga caccaaacca cttattatct ctttcaaata
    11881 ttttatcctt gaaggaatta ttctgcataa gattccagat atgaaaccaa cagaaaaaat
    11941 acttaaaaca attttttcca taatttttcc tctttaccaa tttcttttct tattttttct
    12001 gtcttttcaa ttgatttttt taccaagtcc tcaaaactat aaatcttttt atttttctcc
    12061 ttatcatata ctgcaatcct ttctgtacag caataacatg ggtcaacacc tgcaagaatt
    12121 atacaaacat ctgaaattgt ctcaccaata caacttgctt taaatgttgg aatatttaca
    12181 taacttggtg cccttatttt atgtcttaca ggacttgaag tattaccatc actccttata
    12241 taatgaaaga cctctcctct tggtgcttca tgatgaccta tcccttctcc ttttggtatt
    12301 tctcttactt ctttccatat ttgacctttt ttcccataaa ttgcatcaag tgactgttct
    12361 attatcttta tactttcaaa acattcaagt aatctaacaa ctgccttatc aaaaacatct
    12421 ccattatttg ttgttattac tttccattca acaagattat atgctgcata agggtcatct
    12481 tttcttacat ctatatcaac accagaagcc cttgcaacag gtccaacagc accataattt
    12541 ttaatatctt cttttgttaa aacacctacc ccttttaatc ttgctccaag aacagggtcg
    12601 tcaagaactg cttctgttaa catctgaatt ttccctttaa catctgataa tgttttttta
    12661 agtataggta tatcgctatc ctctat
//
LOCUS       DAOSAT010000052        12580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3375_length_12580_cov_5.529501, whole genome shotgun sequence.
ACCESSION   DAOSAT010000052 DAOSAT010000000
VERSION     DAOSAT010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12580
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3375_length_12580_cov_5.529501"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PLF90_05730"
     CDS             <1..1155
                     /locus_tag="PLF90_05730"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOK56811.1"
                     /translation="GTIISTWQRIGRVGRKREGLVIFMALEDALDQYFIRNPEEFVKR
                     QFEDVIINFENEIISSNHIKCASYEYPLIPDEDRKIYGDILFKIIEKEKFSKTKDGRF
                     FYSGKPIHRDFSLRTVGDMFAIFEIDEERQIGEIEENKVMFECHPGAIYLHHGKKYEV
                     IFINFEKKMVVVEKNNGSYYTQPNWWEKIDILNVEKEKNKDDFLIKFGEIEVTTQVVS
                     YEKRRENDRSLIGTYMLSLPSEKFKTQSLWIEIPDEVVYDIKERRLDFPGSIHAAEHS
                     MIGIFPLVVTCDRVDIGGYSFPFHQQTEKSTIFIYDGYPGGVGITKIAFERIDKIFKV
                     AYDSVLNCKCEMGCPSCIVSPKCGNNNRPLSKSGCLYLLSLLTSGIRPSV"
     gene            1216..2424
                     /locus_tag="PLF90_05735"
     CDS             1216..2424
                     /locus_tag="PLF90_05735"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOK56812.1"
                     /translation="MAIVSIERMKNFMNISHAAEMLLEKNEKEISFSVNLKTRDDEII
                     FCDAYVVYHNVVLGPAKGGIRMAPNVTLEETKRLAKIMTYKNALTELPFGGGKSGIRI
                     PDTNIDEFTRISIFKEYVHAIREEIVSRQYIPAPDLGTDQFDMSVIYGELHIPECVTG
                     KPVKIGGLPGRLEATGRSVATSTRLYVENIMKNKLGNIKVAVQGFGNVGRWTSFFLYK
                     WGAKIVAVSDIYGGVYNEKGFDIEGLFKYAPKKNITISDYPEGEKISNEELLEMDVDV
                     LIPCACESVITKENADKIKSKVIIEGANDPVTEEGDEILKNKGIDVLPDFLCNSGGVI
                     ASYIEWKNAKSGNQTEKEDTFNEIDKKISECFHKICEVKKELKLTYRESATYLSVKFL
                     IEAMHERRWL"
     gene            2787..3503
                     /locus_tag="PLF90_05740"
     CDS             2787..3503
                     /locus_tag="PLF90_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOK56813.1"
                     /translation="MKKVNGFTLIELLVVVAIISILAGMLLPALSKAREKARQITCAN
                     NLKQIGIAFEMYKNDFDDFYPPQQNWKTILWLYYVNPNMRNKIGYCPSRHGRTISYSN
                     WYWGQAYNCGCPSNISRDGNFYPGFAGKKAGQIRNLSKKILVVEWGRSTDGKGGCVAG
                     PPIASYTAGWIGGGTGSYWAVCRIHSGGSNILFGDGRVEWKRPEEYHSNTKDVDNNGN
                     PIPSNPEISSNWREYWDTSY"
     gene            3485..3916
                     /locus_tag="PLF90_05745"
     CDS             3485..3916
                     /locus_tag="PLF90_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOK56814.1"
                     /translation="MGYFLLKLRKFLTLFLLSKFCFSQTIICTTSILSSVVKDIAKDK
                     VNVKTLVPPGICPGHFDIKIEDLKDIEKEGFIFAQGYEPYLEKIKKNIENPYFKIVLI
                     ETEGNSLLPKNQKIIYRKIFDEMAKIFPAYKEYFLKNFKTP"
     gene            3965..4285
                     /locus_tag="PLF90_05750"
     CDS             3965..4285
                     /locus_tag="PLF90_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56815.1"
                     /translation="MKGKKAICNKYIKDLLEYFGFEIIETYGRKEELTPEKIKEIIEK
                     CKIKKLDIVVDNLQAGKDTGKIFAEEFKTPHVVISNFPYAFENTETLRDTLYRDFLII
                     IQKF"
     gene            4288..5010
                     /locus_tag="PLF90_05755"
     CDS             4288..5010
                     /locus_tag="PLF90_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK56816.1"
                     /translation="MAEKIKLWDVSIGYLNQTIVKDVNLKIEKGEIIGIFGPNGAGKT
                     TLICGINGVARIIKGNVFIDNILLTSFSGPYLRRKIGYVPQIIDIDPFLPILTEDVVF
                     MGLYGKKGLFGKIEKEDKKRFDEIADFFEIKNILKKPFGLLSGGEMRKILIVSAFLKE
                     PEILLLDEVFTFLDLRTTKNLFKKIKEIHEKKNLTILIVAHDIYIIENLCQKVIGMEN
                     GKVIFYDEKEKFLKLLKENGNN"
     gene            4997..5809
                     /locus_tag="PLF90_05760"
     CDS             4997..5809
                     /locus_tag="PLF90_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOK56817.1"
                     /translation="MEIIKYAFFQKALVACVLSGIACGIIGVWVVVMRISFIGVSISH
                     SAFAGSLLAILIKKPVQLFSFIFTLITSSILGPFSDRTQLHPETSMGIIFSLTLGLSF
                     LFLGLIPESKSEALNYMWGSILTVDILDIYFLFAITIFVFFFDFLFFREIKALLFNRE
                     LAKANGFYSTIFFYLILYILGLSITFLLKIVGGLLVYALIVNPASSAYQITYNIKKMF
                     FLSVIFGILSTISGLFISSTLNIPTGASIILFSGLLFVFCFLFSPKRRKNEK"
     gene            5799..6386
                     /locus_tag="PLF90_05765"
     CDS             5799..6386
                     /locus_tag="PLF90_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOK56818.1"
                     /translation="MKNKKEFFNNMAENWDKNDKVASNKYRRIIEELKIEDGLKILDI
                     GTGTGVLIPYLLETGKIIEIYAIDYSEKMIEKFREKNFPENVKSFIMDIHNTDFENNF
                     FDRIIANACYPHFEDKEKAIKEIYRILKKNGIFIISHPTGRKFVNELHKKTHPLIKKD
                     IIPDIKKLKSFIEKASFKFGKGIDEEDFFLISFIK"
     gene            6449..7174
                     /gene="thyX"
                     /locus_tag="PLF90_05770"
     CDS             6449..7174
                     /gene="thyX"
                     /locus_tag="PLF90_05770"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOK56819.1"
                     /translation="MEVKLIEMSSNPEEIIYSAARQCYSEKDAYDIFVKAKNIEKKKI
                     EKFIDEIVKSGHESVLEHVKFTFAISGISRALTHQLVRHRVASYSQQSQRYVNMENFK
                     YIVPPSIKKDEILSEIFEETMEFIKNSYKKLIERYKEIGMVGEEANQDARYILPNSAE
                     TKIVVTMNCRELLHFFKVRCCSRAQWEIRQLAYKILEICKEKLPSVFKKGAKCEYLGY
                     CPEGEKFTCGKYPLKKELIKNDR"
     gene            7164..8192
                     /locus_tag="PLF90_05775"
     CDS             7164..8192
                     /locus_tag="PLF90_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56820.1"
                     /translation="MIDELKKVEKKITGIGSLPFLDLKECIDFIKKYFKDIPFLPQLP
                     KLNFKEDMNIQVVENMPGIRIENRKILFEYNEKEILNVYQKIEENEVDYFKISNEYGE
                     GIYEFLKTDIESNFLKGQLVGPLTFLSTAKFKDGKPLIFEEVFEDIFVKIIGMKGIYL
                     SRKIKGKSKIPIIFFDEPVMSSYGSAFFPVEKDRIVNILKNLVNFFRERDKSCLIGFH
                     CCGNTDWSIFFNLDIDILSFDAFGFLENFLIYWEEIEKFIKKERFIAWGIIPSTDEIE
                     NVDFEAIKDKFNFILKYFEKRGVEKKILLSHSIFTPSCGLGYTKEKNSYKNIDYLNNL
                     IDLIQSIL"
     gene            8407..10161
                     /locus_tag="PLF90_05780"
     CDS             8407..10161
                     /locus_tag="PLF90_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOK56821.1"
                     /translation="MNDFEGLEDWIKKRYYAIREKFKKSSFTFNDISNFLFNLYQDSK
                     EQTKLILSELKKAGLLKITKNPEDKRESIYEIVDILETPTLFGKIEEKLTKEQLTNIL
                     KTAADIIRTRVDYTFILLLLFYKALSDKWKNEYEVVKEELMKKGLKENEVIKEAGQPY
                     YHTFNFRQDLLWDGLRKEPIKLPENLSKNLKELSELNPDYKDIFIQFDFHQFTSNPEN
                     QIILNNLFELFSRYSFENVEEDILGDAYEWILKYFAPTKAKEGEVYTPREVIRLMVEI
                     LDPEPEKWIYDPACGSAGMLIVTYQYVKEKYGKEKAEKLFLFGQEYNPKTLALAKMNA
                     ILHRIKNINLIGGDTLLFPKFKEKDSIKKFDYVIANPPWNQDGYDEDTLKKGEYYNER
                     FPFGSPTKQSADWAWIQHMIFSGNKNGKIAIVIDTGAVSRGGREKEIRKKVVENDLIE
                     AVILLPEKLFYNTGAPAVIIVINRNKPEGKKGKVLLINASKEFIPGKAQNILGKENIE
                     KIVNVYRSFKEVEKFSKIINKKDIEDADYNLSPSRFISVFDEENYRPIEEIKEELKKL
                     EEEKRSVEEKIWQILSKF"
     gene            10300..10953
                     /locus_tag="PLF90_05785"
     CDS             10300..10953
                     /locus_tag="PLF90_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOK56822.1"
                     /translation="MERVFIFIDGSNFYHGLKEEIGIRTIDFLKFAFKPCGKRKFVGA
                     NYYNVPLKKEYAPDKYKDQQKFFNYIRNLPGYTLKLGHLELRNVELKKGTVILENSKA
                     HVYFVGPIDKVNEILKKDESKTITQIKYLVEKGVDISIATDMLSLAYDDFYDTGILVS
                     GDGDFAYVIKKIQQLKKKIEVAYFPQRKCWHLRQVSDKFISLEKSFFEDLERKETRK"
     gene            11037..12476
                     /locus_tag="PLF90_05790"
     CDS             11037..12476
                     /locus_tag="PLF90_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK56823.1"
                     /translation="MENKIKKEFLKYLNAIISQQSLKIRGNIVDENGNFYPKRSAFPI
                     FKKYIQDFLKFNSEPRWIIIHGLRGVGKTTLLSQLFYEVLSDKDVEKLYISVDEPMRR
                     FGASLWDIVEGYEILIGKRLEELNKKLFLFFDEVQYDKNWDSAIKTFYDRTKKIFILC
                     TGSSALLLKKQIDANVSRRAYFEELYPMDFKEYIMLKFKKYPEKNLSEKIKGAIFNSK
                     KSEDVFNKLSLLEKKVKEYWFGVDIFEIDRYLKYGTLPFSLKIKEEGVILNQLELMLQ
                     RIIYTDIPEITNFDRESLNKIYSLIYLLSESFEISLTKLSTILEISKDTLSLIFSSLE
                     NANLIQRIPPYGSPYKQVRKPYRYLFTSPAFRFFLLSGMESISNFENFKGKLLEDVVG
                     MYFKKILRKYPNSSLTYDCAKEGANFIVKIKDKKIVIEVGYGKKQIKQALMTLKRITG
                     DYGIVFSLDEEMNFKDNIVKIPIKYFLLI"
BASE COUNT         4862 a         1362 c         2175 g         4181 t
ORIGIN      
        1 ggaacaatta taagtacatg gcagagaatt ggtagagtag gaaggaaaag agaaggtctt
       61 gttattttta tggctcttga agatgcactt gaccagtatt ttataagaaa tccagaggag
      121 tttgtaaaaa ggcagtttga ggatgtaata ataaattttg aaaatgagat aatttcatca
      181 aatcatataa aatgtgcttc ttatgaatat cccttaattc ctgatgaaga tagaaaaatt
      241 tatggagata ttctatttaa aattattgaa aaagaaaaat tttcaaagac aaaggatgga
      301 agattttttt attcagggaa gccaattcac agagatttca gtttaaggac agttggagat
      361 atgtttgcaa tatttgagat agatgaggaa agacagattg gagagataga agaaaataaa
      421 gttatgtttg aatgtcatcc aggagcaatt tatttacatc acgggaaaaa atatgaggtt
      481 atatttataa attttgaaaa gaagatggtt gttgttgaaa agaataatgg cagttactat
      541 acccagccaa actggtggga aaaaatagat attttgaatg tagaaaagga gaaaaataaa
      601 gatgattttt tgataaaatt tggagagata gaagtaacaa cacaggttgt aagttatgaa
      661 aaaaggaggg aaaatgacag gtcacttatt ggaacttata tgttaagttt gccttcagaa
      721 aaatttaaaa cgcagtcatt atggatagaa atacctgatg aagttgttta tgatataaaa
      781 gagagaagac ttgattttcc aggaagtatt cacgcagcag aacattcaat gataggaata
      841 tttcctcttg ttgttacatg tgatagagtg gacattggcg gttattcatt tccatttcat
      901 cagcaaacag aaaaatcaac tatttttata tatgatggtt atcccggagg agttggtatt
      961 acaaaaattg cttttgaaag aatagataaa atttttaaag ttgcctatga ttctgtttta
     1021 aactgtaaat gcgaaatggg ttgtccttcc tgcattgtat ctccaaaatg cggtaacaac
     1081 aaccgtcctt taagtaaatc aggttgcctt tatcttctct ctcttttaac ttcaggaatc
     1141 cgtccttctg tataggattt caagtttttt aaatttataa tataatataa ttcaaaataa
     1201 aaaacggagg ggaaaatggc tatagtttca atagaaagaa tgaaaaattt tatgaatatt
     1261 tcacatgctg ctgagatgct tcttgaaaaa aatgaaaagg agatttcttt ttctgttaat
     1321 ttaaaaacaa gagatgatga aattattttc tgtgatgcat atgttgttta tcataatgtt
     1381 gttcttggac ctgcaaaagg tggaataaga atggctccaa atgttactct ggaagaaaca
     1441 aaacgacttg caaaaataat gacatataaa aatgctttaa cagaacttcc ttttggaggc
     1501 ggaaaatcag gaatcagaat tcctgataca aatattgatg agtttacaag aattagtatt
     1561 tttaaagaat atgttcatgc gataagagaa gagattgttt caagacagta tattccagca
     1621 ccagaccttg gaacagacca gtttgatatg tctgtaattt atggagaact tcatattcct
     1681 gaatgtgtta ctggaaaacc tgtaaaaata ggaggacttc cagggagatt agaagcaact
     1741 ggtcgttcag ttgcaacttc aacaagattg tatgttgaaa atattatgaa aaacaagtta
     1801 ggaaatatta aagtagcagt tcagggtttt ggaaatgttg gtagatggac ctcttttttt
     1861 ctttataaat ggggtgcgaa aatagttgct gtttcagaca tttatggtgg tgtttataat
     1921 gaaaagggtt ttgatattga aggacttttt aaatatgccc ctaaaaaaaa tattacaatt
     1981 tcggattatc cggagggcga aaaaatttca aatgaagaat tacttgaaat ggatgttgat
     2041 gttttaattc cctgtgcctg tgagagtgtg attacaaaag aaaatgcaga taaaataaaa
     2101 tcaaaagtta taatagaagg agcaaatgac cctgtaacag aagaaggcga tgaaattttg
     2161 aaaaataaag gcattgatgt attacctgat tttctctgta attcaggagg agttattgct
     2221 tcctatatag agtggaaaaa tgcgaaatca ggtaatcaaa cagaaaaaga agacactttt
     2281 aatgaaatag ataagaaaat atctgaatgt tttcataaaa tttgcgaagt gaagaaagaa
     2341 cttaaattaa catatagaga atctgctacc tatctttctg taaaattttt aatagaagca
     2401 atgcatgaaa gaagatggtt atgagtcctg tgctcctgtg taatgaagag aaaaaataat
     2461 ttttacagaa tttgagaaag ataggtaata atattttgat aagggtcttt ggtttgtcgt
     2521 attttttttc ttattttact ccctattctt attttctcta tcatcgcctc catttttaac
     2581 ttttctattc tctccaacaa atagcaatct tttcttttta ttttccaaaa agatgaagta
     2641 aattttcaat taaatttttt taatgaagca acgatacctt tttcacttag atttttaatg
     2701 aggcaattcg agattttgac attttaaatt ttttatttta aaatagtaac acgaattttt
     2761 aaaaaataac actaaaggag gaaaagatga aaaaagtaaa tggtttcacg ttgatagaat
     2821 tattagttgt agttgctatt atcagtattc ttgctggtat gcttttgcct gctttatcca
     2881 aggcaagaga aaaagcaaga cagataacat gtgcaaataa tttaaaacag atagggattg
     2941 cttttgagat gtataaaaat gattttgatg atttttatcc accacagcaa aactggaaaa
     3001 caattttatg gctttattat gtaaatccaa atatgagaaa caaaataggt tattgtccaa
     3061 gtaggcatgg tagaacaatt tcatattcaa actggtactg gggtcaggca tataattgtg
     3121 gatgtccttc taatatttca agggatggta atttttatcc agggtttgct ggaaaaaaag
     3181 cagggcaaat taggaatttg agcaaaaaaa tacttgttgt tgaatgggga agaagtacag
     3241 atggaaaagg tggttgtgtt gcaggacctc caattgcttc ttatactgct ggatggattg
     3301 gtggtgggac tggttcttac tgggctgttt gtagaatcca ttcaggagga agcaatatac
     3361 tttttggaga tggaagagtt gaatggaaaa gaccagaaga atatcattca aatacaaaag
     3421 atgttgacaa taacggaaat ccaatacctt caaatcctga aataagttca aactggaggg
     3481 aatattggga tacttcttat taaaacttag aaaattttta actttatttt tactttcaaa
     3541 attttgtttt tcacagacca ttatatgcac aacttcaata ctttcttcag ttgttaagga
     3601 tatagcaaag gataaagtaa atgttaaaac actcgttcct cctggtatat gtcctggaca
     3661 ctttgatata aagattgagg atttaaaaga tattgaaaaa gaaggattta tttttgcaca
     3721 gggttatgag ccatatcttg aaaaaattaa aaaaaatata gaaaatcctt attttaaaat
     3781 tgtccttatt gaaaccgaag ggaattctct tttgcctaaa aatcaaaaaa ttatttatag
     3841 aaagattttt gatgaaatgg ctaaaatttt tcctgcctat aaagaatatt ttttaaaaaa
     3901 ttttaaaact ccttaaaaag aaatagataa aattgataaa aaaataaaag agggagaaaa
     3961 atttttgaaa gggaaaaaag caatctgtaa taaatatata aaagaccttc ttgaatattt
     4021 tggttttgaa attattgaaa catacggaag aaaggaagaa ttgacgccag aaaaaataaa
     4081 ggaaataatt gaaaaatgta aaataaaaaa attagatatt gtagttgata atttacaggc
     4141 aggaaaagat actggaaaaa tttttgcgga agaatttaaa acaccacatg ttgtaatttc
     4201 aaatttccct tatgcctttg aaaatacaga aacattaagg gatacacttt atagagattt
     4261 tttaataata attcagaaat tttaaaaatg gcagaaaaga ttaaactttg ggatgttagt
     4321 attggctatt taaatcagac aattgttaaa gatgttaatt taaaaattga gaaaggagaa
     4381 ataattggaa tttttgggcc aaatggagca gggaaaacaa ccttaatatg tggtataaat
     4441 ggagttgcaa gaattataaa agggaatgtt tttatagata atattcttct tacttctttt
     4501 tcaggacctt atttaagaag aaaaattggt tatgtcccac agattattga tattgaccct
     4561 ttcctcccaa ttcttacaga agatgttgtc tttatgggtt tatatggaaa aaagggttta
     4621 tttggtaaaa tagaaaaaga ggataaaaag agatttgatg aaattgctga tttttttgaa
     4681 attaagaata ttttaaaaaa accctttgga cttctttcag gtggagagat gagaaaaatt
     4741 cttatagtaa gtgcattttt aaaagaacca gaaattcttt tacttgacga ggtttttact
     4801 tttcttgatt taagaacaac aaaaaattta ttcaaaaaaa ttaaagaaat ccacgaaaag
     4861 aaaaatctta caattctaat tgttgctcat gacatttata taattgagaa tttatgccag
     4921 aaagtaatag ggatggaaaa tggaaaagtt attttttatg atgagaagga gaaatttttg
     4981 aaattattaa aagaaaatgg aaataattaa atatgctttt tttcagaaag cactggttgc
     5041 ctgtgtttta tctggaattg cctgcggtat tatcggtgtc tgggttgtcg ttatgagaat
     5101 ttcttttatt ggtgtttcaa tttcccattc agcatttgca ggttctctcc ttgcaatctt
     5161 aattaaaaaa ccagttcaac tcttttcttt tatttttacc cttataacat cttcaatcct
     5221 tggaccattt tcagatagaa ctcaactaca tccagagaca tcaatgggta taattttttc
     5281 tttaacactt ggtttatcat ttttatttct tggtttaatc cctgagagta aaagtgaggc
     5341 acttaattat atgtggggga gtattttaac agttgatatt ttggatattt atttcctttt
     5401 tgctataacg atttttgtct tcttttttga ttttttattt tttagagaga ttaaggcact
     5461 cctttttaat agggaacttg caaaggcaaa tggtttttat tcaacaatat ttttttattt
     5521 aattctttac attcttggtt tatcaataac tttcctttta aaaatagttg gtggacttct
     5581 tgtctatgct ttaattgtta atcctgcatc ttctgcttat cagattactt ataatattaa
     5641 aaagatgttt tttctttctg ttatttttgg tattctttca acaatttcag gccttttcat
     5701 ttcaagtact ttaaatattc caacaggtgc ttcaataatt cttttttcag ggttgctttt
     5761 tgttttttgt ttcctatttt cacctaaaag gagaaagaat gaaaaataaa aaggaatttt
     5821 ttaataatat ggctgaaaac tgggataaga atgataaagt cgcttctaac aaatatagaa
     5881 ggattattga agaacttaaa attgaagatg gtttgaaaat tcttgatatt ggaactggaa
     5941 caggagtttt aatcccttat ttacttgaaa ctgggaaaat tattgagata tatgctattg
     6001 attattctga aaaaatgatt gaaaaattta gagaaaaaaa ttttccagaa aatgttaaaa
     6061 gttttattat ggatattcat aatacagatt ttgaaaataa tttttttgat agaattattg
     6121 caaatgcctg ttatccacat tttgaagata aggaaaaagc aattaaagag atatatagga
     6181 ttttaaaaaa aaatggtatt tttataattt cacatccaac aggcagaaaa tttgtaaatg
     6241 aactccacaa aaaaactcac ccattgataa aaaaagatat aattccagat attaaaaagt
     6301 tgaagtcatt tatagaaaag gcaagtttta aatttggaaa aggaattgat gaagaggact
     6361 tttttctcat ttcttttata aaataaatca aaatattaaa aatttcaaga aaattataat
     6421 ttgttgaaaa atcttaaagg aggtaattat ggaagtgaaa ttaatagaaa tgagttcaaa
     6481 tccagaagaa ataatttatt cagcagcaag gcagtgttat tctgaaaagg atgcatatga
     6541 tatttttgta aaagcaaaaa atatagaaaa gaaaaagatt gaaaaattta tagatgaaat
     6601 tgtaaaatca ggacatgaga gtgtacttga acatgtaaaa tttacttttg caataagtgg
     6661 tatatcaagg gcattaacac atcaacttgt aagacacaga gttgcatcat attcacagca
     6721 aagccagaga tatgtaaata tggaaaactt taaatatatt gttcctccat caattaaaaa
     6781 agatgaaatc ctttctgaaa tttttgaaga aacaatggaa tttataaaaa attcttacaa
     6841 aaaattgata gaaagatata aagaaattgg aatggttggt gaagaagcaa atcaagatgc
     6901 aaggtatatt ctaccaaatt cagcagagac aaaaattgtt gttacaatga attgtagaga
     6961 attattacat ttttttaaag ttaggtgttg tagccgtgct caatgggaaa ttagacaact
     7021 tgcttataag atacttgaaa tatgcaaaga aaaattacca tctgttttta aaaaaggagc
     7081 aaaatgtgaa tatcttggtt attgtccaga aggcgaaaaa tttacatgtg gtaaatatcc
     7141 tcttaaaaag gaattaataa aaaatgatcg atgaacttaa aaaagtagaa aaaaaaatta
     7201 caggcattgg aagtttacca tttcttgatt tgaaagaatg tattgatttt ataaaaaaat
     7261 acttcaaaga tattccattt ttgccacaat taccaaaatt gaattttaaa gaggatatga
     7321 atattcaagt tgttgaaaat atgccaggaa taagaattga aaatagaaaa attttgtttg
     7381 agtataatga aaaggagatt ttaaatgtct atcaaaaaat agaggaaaat gaagttgatt
     7441 attttaaaat tagcaatgaa tatggggaag gtatatatga atttttaaaa acagatattg
     7501 aaagtaattt tttaaaagga caacttgtag gaccattaac ttttctttca actgccaaat
     7561 ttaaagatgg aaagccatta atttttgaag aagtatttga ggatatattt gttaagatta
     7621 ttggaatgaa aggtatatat ctttcgagaa aaataaaagg aaaaagtaaa atccctataa
     7681 ttttttttga tgaaccagtt atgtcaagtt atggttctgc ttttttccca gtagaaaaag
     7741 atagaatagt taatatactt aaaaatttag ttaatttttt tagggaaagg gataaaagtt
     7801 gtttaatagg ttttcattgt tgtggtaata ctgactggtc tatatttttt aaccttgata
     7861 ttgatatttt gagctttgat gcttttggtt tccttgaaaa ttttttaatt tattgggaag
     7921 aaatagaaaa atttattaaa aaggaaagat ttattgcatg gggtattatt ccttctacag
     7981 atgaaattga aaatgttgat tttgaagcga taaaagataa atttaatttt atcttaaaat
     8041 attttgaaaa aagaggagtt gagaaaaaaa tattactctc acattcaatt tttaccccat
     8101 catgcggact cggttataca aaagagaaga attcctataa aaatatagat tacttgaata
     8161 accttattga tttaattcaa tcaattttgt aaattttttt attttatctg acccacatat
     8221 aaaacagttg aaccggaaaa tttttagcat tttttacttc tagattataa tttttcacca
     8281 tacattttag taaaaaaaag aagattaatg tgataatgaa atttaatctt tgaaactaaa
     8341 catacacttg acaaggaaat aattttaagg tataataagt taagtaaaat aaaaaaggtt
     8401 aaccaaatga atgattttga aggtttggaa gattggataa agaaaaggta ctatgcgata
     8461 agagagaaat ttaaaaaaag ttctttcaca tttaatgata tttcaaattt tctttttaat
     8521 ctttatcagg actcaaaaga acagacaaag cttattttat ctgaattaaa aaaagcagga
     8581 cttcttaaaa taacaaaaaa tccagaagac aaaagggaat caatttatga aattgttgat
     8641 attcttgaaa cacctactct ttttgggaaa atagaagaaa aactaacgaa ggaacaactt
     8701 acaaacattc taaaaacagc agcagatata attaggacaa gggttgatta cacttttatt
     8761 ttacttttac ttttttacaa ggcactctct gataaatgga aaaatgaata tgaagttgtc
     8821 aaagaagaat tgatgaaaaa gggattgaaa gaaaacgaag taataaagga ggcaggacaa
     8881 ccatattatc acacatttaa cttcagacag gacttattat gggatgggtt aagaaaagaa
     8941 ccgataaaac ttcctgaaaa cctatcaaaa aatttaaaag aattatctga attaaatcct
     9001 gattataagg atattttcat tcagtttgat tttcatcaat ttacctcaaa tccagaaaat
     9061 cagataattt taaataatct ttttgaactc tttagtagat actcttttga aaatgttgag
     9121 gaagatatac ttggagatgc ttatgagtgg atacttaaat attttgctcc aacaaaggca
     9181 aaggaaggag aggtttatac gccaagggaa gttattagat tgatggttga aattttagac
     9241 cctgaaccag aaaaatggat atatgaccct gcctgtggaa gtgctggaat gcttatagtt
     9301 acctaccagt atgtgaagga aaaatatgga aaggaaaagg cagaaaaact attcttattt
     9361 ggacaggaat acaatccaaa aacacttgca cttgccaaaa tgaatgctat cttacacaga
     9421 ataaaaaata taaacttaat cggtggggat acacttttat ttccaaaatt taaagagaaa
     9481 gatagtataa aaaaatttga ttatgttatt gcaaatcctc catggaatca agatggttat
     9541 gatgaagaca cattaaagaa aggagaatat tataatgaaa gatttccatt tggttctcca
     9601 acaaaacaat ctgctgattg ggcatggata caacatatga ttttttcagg taataaaaat
     9661 gggaaaattg caattgttat tgatacagga gcggtttcaa ggggagggag ggaaaaagag
     9721 ataagaaaaa aagttgtaga aaatgattta attgaggcag ttatactttt accagaaaaa
     9781 cttttttaca atacaggagc accagcagtg ataattgtaa taaatagaaa taaaccagaa
     9841 ggtaaaaaag ggaaggtttt attgataaat gcatcaaaag agtttattcc ggggaaagca
     9901 cagaatatac ttggaaagga aaatatagaa aaaatagtaa atgtttatag gagttttaaa
     9961 gaggttgaaa aatttagcaa aattataaat aaaaaagata ttgaagatgc tgactacaat
    10021 ttatctccct caagatttat aagtgttttt gatgaagaaa attatagacc aattgaagaa
    10081 ataaaagaag aactaaaaaa acttgaagaa gaaaaaagaa gtgtagaaga aaaaatatgg
    10141 caaattttat caaaatttta aaattagttg cacttgacaa aaaatttttt ggaaagtatt
    10201 atataattaa agtattactt ctttgccttt ggtttaccaa aggcaaattt ataacctccc
    10261 cgggcttgcg aaagcaatcc cgggtttttt tattttataa tggagagagt atttattttt
    10321 attgatggaa gtaattttta tcatggactt aaagaggaaa ttggtataag aaccattgac
    10381 tttttaaaat ttgcttttaa accctgtgga aaaagaaaat ttgttggtgc aaactattat
    10441 aacgtacctt taaagaaaga gtatgctcca gacaagtata aggatcaaca aaaatttttc
    10501 aattacatta gaaatttacc tggatacact cttaaacttg ggcatcttga attgagaaat
    10561 gttgaattaa agaaaggtac tgtaatttta gaaaattcaa aagcacatgt atattttgtg
    10621 ggacctattg ataaggtaaa tgaaattctc aaaaaagacg aatcaaaaac aataactcaa
    10681 attaaatatt tagtagagaa aggagttgat atatctatag caactgatat gctttcactt
    10741 gcatatgatg atttttacga tacaggaatt ttagtaagtg gtgatggcga ttttgcttat
    10801 gtaattaaga aaatacagca attgaagaaa aaaatagaag tggcatattt cccgcagaga
    10861 aaatgttggc atctaaggca ggtaagcgat aagtttattt cattagaaaa atcttttttt
    10921 gaagatttgg aaagaaagga aacccgaaaa tgaaaagaaa caaaatttta aatttactat
    10981 acgactataa tcggatagta ttgacatttt ctatacgact atggtagtat agtaatatgg
    11041 aaaataaaat aaaaaaagaa tttttaaagt atttgaatgc cataatatca caacagagtt
    11101 taaaaattag gggcaatatt gttgatgaaa atggtaattt ttatccaaaa agaagtgctt
    11161 ttcctatttt taaaaaatat atccaggatt ttttaaaatt caattctgaa ccaagatgga
    11221 taataataca tggacttcgt ggagtaggaa aaacaacatt attatcgcaa cttttctatg
    11281 aagtattatc agataaagat gttgagaaac tttatatttc agttgatgaa ccgatgagaa
    11341 gatttggtgc atcattatgg gatattgttg aaggatatga aatattaatt gggaaaaggt
    11401 tagaagaatt aaataaaaaa ttatttttat tttttgatga agttcaatat gataaaaact
    11461 gggattctgc tatcaaaaca ttctatgata ggacaaaaaa aatttttatt ttatgcactg
    11521 gttcttctgc cttattattg aaaaaacaga ttgatgcaaa tgtttcaaga cgtgcatatt
    11581 ttgaggaatt atacccgatg gattttaaag aatatataat gttaaaattc aaaaaatatc
    11641 cagaaaaaaa tttaagtgag aaaattaaag gtgctatttt taattctaaa aaaagcgaag
    11701 acgtttttaa taagttatcc ttattagaga aaaaagtaaa agaatactgg tttggagttg
    11761 atatttttga aatagataga tacttgaaat atggaactct tcctttttct ctaaaaatta
    11821 aagaagaggg agtgatttta aatcaactgg aattgatgtt acagagaatt atttatacag
    11881 atattcctga aataacaaat tttgatagag aaagtttaaa caaaatttat tctctcattt
    11941 atcttctttc tgaaagtttt gaaataagtt taacaaaatt atctacaata cttgaaattt
    12001 caaaagatac cctttcttta attttttctt ctcttgaaaa tgcaaattta atccaaagaa
    12061 ttccaccata tggttcacct tataagcagg taaggaagcc atatagatat ctttttacaa
    12121 gtcctgcttt tcgttttttt cttctgtcag gtatggagag tataagtaat tttgaaaatt
    12181 tcaaagggaa attactggaa gatgttgtag gaatgtactt taaaaaaatt ttgagaaaat
    12241 atccaaattc ctctttaact tatgattgtg caaaagaagg agcaaatttt attgttaaaa
    12301 taaaggataa aaaaattgtt attgaggttg gttatggcaa aaagcaaatt aaacaagcac
    12361 tgatgacttt aaaaagaata actggcgatt atggaattgt gttttcgtta gatgaagaaa
    12421 tgaattttaa agataatatt gtaaagatac cgattaaata ttttttactc atttgaggtg
    12481 gaatttttat gaggagattg gggattgaaa gaaaaatttt acaaaatttt aaaaaagaag
    12541 gtaaaattaa aatgaacttc cctggaggtt tttaccttca
//
LOCUS       DAOSAT010000053        12570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3380_length_12570_cov_5.525849, whole genome shotgun sequence.
ACCESSION   DAOSAT010000053 DAOSAT010000000
VERSION     DAOSAT010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12570
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3380_length_12570_cov_5.525849"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="PLF90_05795"
     CDS             <1..1013
                     /locus_tag="PLF90_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOK56824.1"
                     /translation="ILENNRFIEPFEVVTDLYGRPVYKNIDPTGPLSIFFAISFGFCL
                     TDAGYGILLVLISLILMKKFRFLPNFIKFLKLLLICGISTLIIGSLTGGWFGDIIYKL
                     PESISFVKYLKKLVLLNPLESGNKSILFLSIALAFGYIQIIWGLILNLYNQIRRYSLK
                     YCGEAITLLLIQILVGIIVIGFLKMKILMKVPLYILLLCFIYLMYEKAKTQKEFMLKG
                     FWAVYGAYSVIAGNLLGDVLSYSRLFGLGLTTSVLGLVINQMVSMVKNIPFVGIFISA
                     LIFIVGHFGNLVINLLGSYVHTSRLQYLEFFTKFFEGGGRVFTPFKEVRNYTIFKTNR
                     EN"
     gene            1059..1718
                     /locus_tag="PLF90_05800"
     CDS             1059..1718
                     /locus_tag="PLF90_05800"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56825.1"
                     /translation="MGGERVGMVNELISKFWLAFSKVIGGLLIFLIGWLIAKFLKVLI
                     IKFFKVIKIDKLSEDSGLKELLDKGNISMDVSQLLGIGVYWLLMLVVVFLAINFAGIQ
                     IPSTVIDKILGFIPRFILGLLIFIFSIFLGSFFGGIVRTSAGNAGIEKAPFLGKLTQI
                     AIVVFGTVLALQEIGIAADFIGDVFIIVLTSFCFGTALAFALGTKDIIKEKVEEFLKK
                     K"
     gene            1726..1797
                     /locus_tag="PLF90_05805"
     tRNA            1726..1797
                     /locus_tag="PLF90_05805"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1758..1760,aa:Ile,seq:gat)
     gene            1836..2525
                     /locus_tag="PLF90_05810"
     CDS             1836..2525
                     /locus_tag="PLF90_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOK56826.1"
                     /translation="MEKVVFVAEIREGKGKGTARKLRSKGYIPGILYGPDTEPVPIKL
                     EKKSSEKIILHLTSHNIMAEMKLKKNGQEETIQVVLKDIQTDPIKDEIIHLDFYKLSS
                     ERPVIMEIPVIIKGKSIGEEKGGILEHELREIKVEGLPGNIPENIEIDIKDLDFGHAI
                     LVKDIKMPEGVKILEDENRVVVTVLKPKEEKVEEVKVEETVEEPKVISQEKVEERRRE
                     KEEAQKEESQK"
     gene            2522..3079
                     /gene="pth"
                     /locus_tag="PLF90_05815"
     CDS             2522..3079
                     /gene="pth"
                     /locus_tag="PLF90_05815"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOK56827.1"
                     /translation="MIFVGLGNPGKEYSETRHNVGFKVVDRLKKSGRVIEIYKKKLFN
                     GWKVEIEGKEIIILKPKTYMNESGKAVKIALDFFNEKVENLFLIHDELDIEIGKIKII
                     KDKGPGGHKGVISVMENIQSKDFVRIRVGIRGEKIETSYIDYVLSPFLPEEKEIIEKA
                     IDKSVDAIKEILRSNLEKAMSLYNN"
     gene            3093..5186
                     /locus_tag="PLF90_05820"
     CDS             3093..5186
                     /locus_tag="PLF90_05820"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOK56828.1"
                     /translation="MDYSLLITIIGSVFALGYTYYLAKFILKRPEGTPEMIEVAEAVR
                     TGARAYLKRQYKGVGVFFSVIFIILLLLSIFNYLPIFVPFAFLTGGFFSALSGYIGMT
                     IATRSSARTAFAAKNSLNSALRIAFSAGLVMGLVVVGLALLDISIWYNLLTWYYRSRG
                     YGIEERLRYITSTMICFGMGASAQALFARVGGGIYTKGADVGADLVGKVEAGIPEDDP
                     RNPAVIADNVGDNVGDVAGMGADLYESYTDAIIATMALAYAAGLGLKGVSLPMLIATI
                     GVISSIIGSFLVKASEDASQANLLRALRRGIYFASILVVIFSYPAVVLLLGYQYKGLY
                     VSILSGIIAGVIIGFFTEFFTSDTYPPTKAVAKSALTGHATVIIEGISTGMLSTIAPV
                     LTVVIATICAYVFAGGLYEPELGLYGIGLAGVGLLSTLGITLATDAYGPVADNAGGNA
                     EMTHQPPFVRQRTDALDALGNTTAATGKGFAIGSAALTALALIAAYKDQIVLLGGKLD
                     MSIMNFRVLCGIFIGAMYPFFFCSMALKAVGRAAQQIVQEVRRQFKEISGLLEGKAKP
                     DYAKAVDICTKSAQKEMLLPSILTVLLPIIVGILLGVDGVVGLLVGALTSGFAVAIMM
                     ANSGASWDNAKKYIEKGNFGGKGTDVHKASVTGDTVGDPFKDTAGPSLNILIKLISIV
                     SIVFAGFIVKNSIFK"
     gene            5183..6337
                     /locus_tag="PLF90_05825"
     CDS             5183..6337
                     /locus_tag="PLF90_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004404901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOK56829.1"
                     /translation="MKNLSKEALKLHKKAKGKLEIKPKVPLKTKKDLSLAYTPGVAIP
                     CLEIAKEKNKIYEYTWKGNTVAIVTDGSAVLGLGNIGPHAALPVMEGKAILFKEFGEV
                     DAVPLCLDTQNVEEIIDIVKKISPCFGGINLEDISAPRCFTIEQRLNEELEIPVFHDD
                     QDGTAIVVLAALINALKLVNKKIEDIKIVINGAGAAGIAITKMLYKYGARKIVLCDRE
                     GIIYKGRKENMNFVKEEITDYLLESKKGSLKDAMEKTDVFIGVSGPNLVTKEMVKSMN
                     KYSIVFAMSNPVPEIMPDEAKKGGAYIIGTGRSDFPNQINNLLAFPGIFRGTFDAKAK
                     KITEKMKISASLAIANYIPENKLSTDYIIPSALDKNVAKQVAKAVKKSAL"
     gene            complement(6357..7847)
                     /locus_tag="PLF90_05830"
     CDS             complement(6357..7847)
                     /locus_tag="PLF90_05830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOK56830.1"
                     /translation="MVENKKDCSFLWIFSVTLILKLFLSYFFPLTGDEAYFITTGKIF
                     DFGYYEHPPMIWWIIYIFSLCGRYTFHFFYYRLFSIFSTLIIVFFISRLLTEKKNSFL
                     IPSFFLLSPVYLLSFLITNDIPLLLFTFLSGVFFYKGIEKEKRIFFVISGFFFGLAFL
                     SKYLSVLYLFGVFFYVIFKNEKKYWKNFIIFILSSLPLIFINFYWNYNHCWINILFNL
                     VYRKKVEYFKIKNIILFFLSLFFLMTPYFFFLFLKEKFFLKRKFETGYDFFFYVFLIP
                     LFFLFLMSFLRVVGLHWYISFVPFSFLLLKDFEEDKISKSIKISIYFNGFIILVIISI
                     LLCPLKMFKNHKEFSEIIMFKNPYPICAYLEKFKNEYIFATPGYTETAIMSYYCKKDF
                     IVFGDLGHSGREYDITFDFKKIDGKDILIFSPFDVEEAKYLEFFTEIKKEKIVIEGGN
                     FYLLFCKKFNYENYRNIILRQTYEKFYKIPEFLPVKKNFFKEKYNF"
     gene            complement(7841..8275)
                     /gene="ruvX"
                     /locus_tag="PLF90_05835"
     CDS             complement(7841..8275)
                     /gene="ruvX"
                     /locus_tag="PLF90_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOK56831.1"
                     /translation="MRILCIDVGDKNIGIAISDEENILAKGLGSLKMNDDIVARIKEI
                     IKKYNIGRIVYGLPLKMDGSFSVQTEKTLAFISRLKEAIKDIEFISFDERLTTSLAEK
                     FLLEADLSRKKRKKYIDKLSAQIILQNYLDSINVKNDEEIWW"
     gene            complement(8470..9270)
                     /locus_tag="PLF90_05840"
     CDS             complement(8470..9270)
                     /locus_tag="PLF90_05840"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOK56832.1"
                     /translation="MFRFIIVFLFIVLMNITPLSFGNGIGPPSPEVLKKIEELKTIKE
                     NIEKSEGDLKKKWIQKLIKIFEREIEYLKTYDYKPDSHKVMPDEENYFRYYFHELSFE
                     VGQLPNLDEKIIKLLIGYPTYPYKHPPYTYQLIKTKDKEWILKHFTICANDPDDNVRD
                     SAICELSLISNPFIESAEWDLTPYEKIHRALSSEDKNLLKKVLTKALEDPCFTIRLSA
                     IRGLIIYKDDREIISKIEKIAKEDNHEYVREKAQKLLKEMKTQSNKFF"
     gene            complement(9344..9544)
                     /locus_tag="PLF90_05845"
     CDS             complement(9344..9544)
                     /locus_tag="PLF90_05845"
                     /inference="COORDINATES: protein motif:HMM:NF019896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HOK56833.1"
                     /translation="MPVSSGYRNPIHNEEVGGKSESRHMYGDAVDIRLQVGMGMEIYI
                     MMIGNLCMILQKKKELPILIQN"
     gene            complement(9552..10103)
                     /locus_tag="PLF90_05850"
     CDS             complement(9552..10103)
                     /locus_tag="PLF90_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56834.1"
                     /translation="MNLDIFLDPETEEPLDYLIADGESQALNKVKIQYNSTPLPNHTI
                     SWKFKFWKYDTILITMDSSAQIIYNKSLWDLEDEEWEGVFNLSFEFADPDYEGTSDIQ
                     YGSITPTSQTNQNGIATSIFTAGTEAGFIEIWVCDNDFRIVERGSFHIRFPWRKGEKG
                     QQKPSAPSGRGFPLAYKIVARFR"
     gene            complement(10261..10464)
                     /locus_tag="PLF90_05855"
     CDS             complement(10261..10464)
                     /locus_tag="PLF90_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56835.1"
                     /translation="MICAGGNVNPNLDIHKAEIIATTTPAIEGIQINFEITGHKGYDS
                     IKDETEYNINGADNGKFSNGGTK"
     gene            complement(10831..11004)
                     /locus_tag="PLF90_05860"
     CDS             complement(10831..11004)
                     /locus_tag="PLF90_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56836.1"
                     /translation="MRYFCTDGVKIKFFLFFILCSILLYGGVSDTLKEIKENVKNKEY
                     KKATEKSLSNLCN"
     gene            complement(11101..11940)
                     /gene="folP"
                     /locus_tag="PLF90_05865"
     CDS             complement(11101..11940)
                     /gene="folP"
                     /locus_tag="PLF90_05865"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOK56837.1"
                     /translation="MNWKIKDFVFNIEKPLIMGILNVTPDSFYDGGKFFNLDKAVERG
                     IEIEKEGADIIDIGGQSTRPGSKQVDEKEEIERVIPVIKVLSKELKIPISCDTFRSKV
                     AEKAIENGAKIINDISAFSMDKNLLDVIKNSDCGYVLMHMKGTPENMQINPYYEDVMG
                     EISEFFEEKLKILEENKVNIERVVIDPGIGFGKRVCDNIEILKNLDKFKKFNRPILIG
                     TSRKSFIGKILNLEVEERLSPTIATNVYAYLKGAGIFRVHDVKEVKNAIDIIYWIEKV
                     VEI"
     gene            complement(11937..>12570)
                     /locus_tag="PLF90_05870"
     CDS             complement(11937..>12570)
                     /locus_tag="PLF90_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOK56838.1"
                     /translation="KLTEYGKKLVEKGLVAGPGGNISARDGDFVYLSPSGFFLDEIKE
                     EEWVKVDLKTGEIFGNLRPTCEISMHLGIYVIRENVRAVIHTHPPITVGLISAGVKFK
                     PFFPDYVAILGKDVPVIDYVPPAGEEIRKAVCKEIKNVNVVLLKNHGVVAVGESIKEA
                     YTRSLIVEEAAKSIFAGILSGKLRYFTKKEIEQIENLEAEDYRKALLKNQ"
BASE COUNT         4306 a         1904 c         1980 g         4380 t
ORIGIN      
        1 taattcttga aaacaataga tttattgaac catttgaagt tgttactgac ttatatggta
       61 gacctgttta taaaaatatt gacccgacag gacctctatc catatttttt gctatttcat
      121 ttggattctg tttaacagat gctggatatg gaattttact tgtattaata tctctgattt
      181 taatgaaaaa attcagattt ctgccaaact ttattaaatt tttaaaatta ctgcttatat
      241 gtggaatttc aactttaata atagggtctt taactggtgg atggtttgga gatataatat
      301 ataaacttcc agaaagtatt tcttttgtta aatatctaaa aaaattagta cttttaaatc
      361 cacttgaaag tgggaataaa tccattctat ttctttcaat tgctcttgcc tttggctata
      421 tccagattat atggggtctt attttaaatc tttacaatca aataagaaga tattctctta
      481 aatattgcgg agaagcaatt acacttcttt taatccagat acttgttgga attattgtta
      541 ttggatttct taaaatgaaa atattaatga aagtgcctct ttatatttta cttttatgtt
      601 ttatttatct gatgtacgaa aaagcaaaaa ctcagaaaga atttatgtta aagggtttct
      661 gggctgttta tggtgcttac agtgttattg cgggtaattt actcggagat gtattaagtt
      721 acagccgtct ttttggtctt ggattgacaa catcagttct tggacttgta atcaatcaaa
      781 tggtctcaat ggtaaaaaat attccttttg taggaatttt tatatccgct cttattttta
      841 tagttgggca ttttggtaat ctcgtaatca atctactcgg aagttacgtt catacaagca
      901 gattacagta tcttgagttt tttacaaagt tttttgaagg tggaggaagg gttttcactc
      961 catttaaaga agtcagaaat tatacaatct ttaaaacaaa tagagaaaat tgattttttt
     1021 taattttatg atagaataat ccaaaaaagg agggaataat gggtggagaa agagttggga
     1081 tggtaaatga actgattagt aaattctggc ttgctttttc aaaagttatc ggagggcttt
     1141 taatatttct gattggctgg ctcatagcta aatttctgaa agtattaata ataaagtttt
     1201 tcaaagtaat aaaaattgac aaactttcag aagattcagg gcttaaagaa ttgcttgata
     1261 aaggtaatat atcaatggat gtctcacaat tactcggaat aggtgtttac tggttgttaa
     1321 tgcttgttgt tgttttcctt gctattaatt ttgcaggaat acagatacca tcaaccgtta
     1381 tagacaaaat tcttggattt attccaagat ttatacttgg acttttgata ttcattttct
     1441 caattttcct tggcagtttc tttggcggta ttgtaagaac atctgctgga aatgcaggaa
     1501 ttgaaaaagc accattttta gggaaattaa ctcaaatcgc cattgtagta tttggaactg
     1561 ttcttgcttt gcaggaaata ggaattgcag cagattttat tggagatgtt tttattatag
     1621 ttcttacttc tttctgtttt ggaactgctc ttgcttttgc tctgggtaca aaggatatta
     1681 ttaaagaaaa agtagaagaa tttttaaaga aaaagtaaaa ttttagggcg cgtagctcaa
     1741 gggcagagca ccgtgctgat aacgcggggg ctggtggttc gaatccatcc gcgcccattt
     1801 ttttaaagaa atatatataa ataaaggagg acttcatgga aaaagtagtt tttgtggcag
     1861 aaataagaga aggaaaaggt aaaggaactg cgagaaaatt aagaagtaaa ggatatattc
     1921 ccggtatttt atatggacct gatacagaac ctgtccctat aaaacttgaa aaaaaatcaa
     1981 gtgagaaaat aattttacat ttaacatccc acaacattat ggctgaaatg aaacttaaaa
     2041 aaaatggaca ggaagagaca attcaggttg ttttaaaaga tatacagaca gaccctataa
     2101 aagacgaaat aattcatctt gacttttaca aattgagttc agaaaggcct gttattatgg
     2161 aaatcccggt tattataaaa ggaaaatcaa ttggagaaga aaaaggtgga atacttgaac
     2221 atgaattgag agaaataaaa gttgaaggac ttccaggaaa tattcctgaa aatatagaaa
     2281 ttgatataaa agaccttgat ttcggacatg caattttagt taaagatata aaaatgcctg
     2341 aaggagttaa aattcttgaa gatgaaaaca gagttgtagt tacggttctg aaaccaaaag
     2401 aagaaaaagt agaagaagtt aaagttgaag agacagtaga agaaccaaag gtaatttcac
     2461 aggaaaaagt tgaagaaaga agaagagaaa aagaagaagc acagaaagaa gaaagtcaga
     2521 aatgattttt gttggacttg gtaatcctgg aaaagaatat tctgaaacaa gacataatgt
     2581 tggttttaaa gttgttgata gattaaaaaa aagtggaaga gttattgaga tttataagaa
     2641 aaaacttttt aatggctgga aagttgaaat tgaaggcaag gaaataataa ttttaaagcc
     2701 aaaaacatat atgaatgaaa gcggaaaagc agtaaaaatt gctcttgact tttttaatga
     2761 aaaagtagaa aatctatttc ttattcatga tgaacttgat atagaaattg ggaaaataaa
     2821 aataataaag gataaaggac caggaggaca taaaggagtg atttctgtta tggaaaatat
     2881 acagagtaaa gattttgtta gaataagagt aggaataaga ggagaaaaaa tagaaacaag
     2941 ttatattgat tatgttttat ctccgttttt accagaggaa aaagagatta tagaaaaagc
     3001 aatagataaa tctgtggatg caataaagga aatattaaga agtaacttgg aaaaggcaat
     3061 gagtttatat aataactgaa aggaggaaaa aaatggatta ttctttattg attacaatta
     3121 taggttcagt ttttgcactc ggttatacct attatttagc aaaatttatt cttaaaagac
     3181 ccgaaggaac acctgaaatg atagaagttg cagaagctgt aagaaccggt gcaagagcat
     3241 atttaaaaag gcaatacaaa ggagttggag tatttttcag tgttattttt attatacttt
     3301 tattactatc tatttttaat tatctaccta tttttgtccc ttttgctttt ttaacaggtg
     3361 gttttttctc tgctttaagt ggatatattg gaatgacaat tgcgacaagg tccagtgcaa
     3421 gaactgcatt tgctgctaaa aattctttaa atagtgcttt aagaattgct ttttctgcag
     3481 gacttgttat ggggcttgtt gttgttggac ttgcactttt agatataagt atctggtata
     3541 accttcttac ctggtattac agaagtagag gatatggaat tgaagaaaga ttaagataca
     3601 taacctccac aatgatatgt tttggtatgg gagcaagtgc acaggcactt tttgcaagag
     3661 ttggaggagg tatttataca aaaggagcag atgttggagc agaccttgtg ggaaaagttg
     3721 aagcaggaat tcctgaagat gacccgagaa acccggcagt tattgcagat aatgtaggag
     3781 ataatgtagg agatgttgct ggaatgggag cagaccttta cgaatcatat acagatgcaa
     3841 taatagcaac aatggctctt gcttatgcag caggacttgg acttaaaggt gtctcccttc
     3901 ctatgttaat tgcaacaata ggagttattt catcaataat aggttctttt cttgtcaaag
     3961 caagtgaaga tgcaagtcag gcaaatttat taagggcttt aagaagaggt atttattttg
     4021 cttcaatttt agttgttata ttttcctatc ctgcagttgt attacttctt ggttaccagt
     4081 ataaaggact ttatgtttca attttaagtg gaataatagc aggtgttata attggatttt
     4141 ttacagaatt ttttacatca gatacctatc caccaacaaa ggcagttgct aaaagtgctt
     4201 taacaggaca tgctactgta attatagagg gtatttcaac aggaatgtta tcaacaattg
     4261 ctcctgtttt aaccgttgtt attgccacaa tctgtgctta tgtttttgca ggtggacttt
     4321 atgaacctga actcggactt tacggaattg gacttgctgg agttggactt ttgagtactc
     4381 ttggaattac acttgcaaca gatgcttatg gacctgttgc ggataatgca ggaggaaatg
     4441 cagaaatgac acatcagcca ccatttgtta gacagaggac agatgctctt gatgctcttg
     4501 gaaatacaac tgctgcaaca ggtaagggtt ttgccattgg ttctgctgct ttgactgctc
     4561 ttgctctaat tgctgcatat aaggaccaga ttgttttact cggtggaaaa cttgatatgt
     4621 caataatgaa ttttagagtt ttatgcggta tttttattgg tgccatgtat ccattctttt
     4681 tctgctcaat ggctttaaaa gcagttggaa gagcagcaca gcagatagtt caggaagtaa
     4741 gaagacagtt taaagaaatt tcaggacttc ttgaagggaa agcaaaacct gattatgcaa
     4801 aagcagttga tatatgtaca aaatcagcac agaaagaaat gcttcttcct tcaatattaa
     4861 cagtccttct tcctataatt gttggaattt tacttggagt tgatggagta gttggacttc
     4921 ttgtaggtgc tttaacaagt ggatttgctg ttgctataat gatggcaaat tcaggtgcat
     4981 cctgggataa tgcaaaaaaa tatattgaaa aaggaaattt tggtggaaag ggaacagatg
     5041 ttcataaagc atctgttaca ggagatacag ttggagaccc gtttaaggat acagcaggac
     5101 cttctttaaa tattcttata aaattaattt caattgtttc aattgttttt gctggattta
     5161 tagttaaaaa ctcaatattt aaatgaagaa cttatcaaaa gaagcgctta aactgcataa
     5221 gaaagcaaaa ggtaaacttg aaataaaacc gaaagttcct ttaaaaacaa aaaaagattt
     5281 atctcttgcc tatactcctg gagttgctat tccctgcctt gaaatagcaa aagaaaaaaa
     5341 caaaatttat gaatacacat ggaaaggaaa tactgttgct attgttactg atggaagtgc
     5401 agttttaggt cttggaaata tcggacctca tgcagcacta cctgttatgg aagggaaggc
     5461 aattttgttt aaagaattcg gtgaagttga cgcagttcct ttatgtcttg atactcaaaa
     5521 tgttgaagaa ataattgata tagtgaaaaa aatatctcca tgttttggtg gaataaatct
     5581 tgaagatatt tctgctccaa gatgttttac cattgaacag agattaaatg aagaacttga
     5641 aattccagtt tttcatgatg accaggatgg aacagcaata gttgttcttg ctgctttaat
     5701 aaatgcttta aaacttgtga ataaaaaaat agaagatata aaaattgtaa taaatggagc
     5761 aggagcagca ggtatagcaa taacaaaaat gttatataaa tacggtgcaa gaaaaattgt
     5821 tttatgtgat agagagggaa taatttataa aggaagaaaa gaaaatatga actttgtaaa
     5881 agaagaaata acagattatc tacttgaaag caaaaaaggc agtttaaagg atgcaatgga
     5941 aaaaacagat gtttttatag gagtttcagg tccaaatctt gtcacaaaag aaatggttaa
     6001 aagtatgaat aaatattcta ttgtttttgc aatgagcaat ccagtccctg aaataatgcc
     6061 agatgaagca aaaaaaggtg gtgcatacat aatcggaaca ggaagaagtg atttcccaaa
     6121 tcagataaac aatttacttg cttttccggg cattttcaga ggtacttttg atgcaaaggc
     6181 aaaaaaaata acagaaaaaa tgaaaatatc agcaagttta gcaatagcaa attatattcc
     6241 tgaaaacaaa ctttctactg attatataat tccttctgct cttgataaaa atgttgcaaa
     6301 gcaggttgca aaagcagtaa aaaagtccgc tctttaatca acaattttga ttttctttaa
     6361 aaattatatt tttccttgaa aaaatttttc tttacaggta aaaattccgg tatcttataa
     6421 aatttctcat atgtctgtct taaaattata tttctataat tttcataatt gaatttttta
     6481 cagaaaagca aataaaagtt cccgccttca ataacaattt tttctttttt aatctctgtg
     6541 aaaaattcta aatattttgc ttcttctaca tcaaatggag aaaaaattaa aatatccttt
     6601 ccatctatct ttttaaaatc aaaagtaata tcatattctc ttccgctgtg tccgaggtct
     6661 ccaaaaacaa taaagtcctt tttacagtaa taagacataa ttgctgtttc tgtataaccg
     6721 ggagttgcaa aaatatattc atttttgaat ttctcaagat aagcacagat gggatatgga
     6781 tttttaaaca ttattatttc agaaaactct ttgtgatttt taaacatttt gagaggacat
     6841 aaaaggattg aaattataac gagtataata aatccattaa aataaataga aatttttatg
     6901 gattttgaaa ttttatcttc ttcaaaatct ttcagaagta aaaaagagaa aggaacaaag
     6961 gatatatacc agtgaagccc caccactctt aaaaaggaca ttaaaaacag aaaaaataaa
     7021 ggaattaaaa aaacataaaa gaaaaaatca taacctgttt caaatttcct ttttaaaaaa
     7081 aacttttctt ttaaaaaaag aaagaaaaaa taaggggtca taagaaaaaa caaagagaga
     7141 aaaaacagaa ttatattttt aattttaaaa tattccacct ttttcctgta aacaagatta
     7201 aagagaatat ttatccagca gtgattatag ttccagtaaa aattaataaa aatcaaagga
     7261 agagaagaaa ggataaaaat tataaaattt ttccagtatt tcttttcatt tttaaaaata
     7321 acataaaaga aaacaccgaa aagataaaga acagacaaat atttactcaa aaaagcaaga
     7381 ccgaaaaaaa atcctgaaat aacaaaaaaa atcctttttt ctttttcaat ccctttataa
     7441 aaaaatactc ctgaaagaaa agtaaaaaga agtaatggta tatcatttgt aattaaaaaa
     7501 ctcaaaagat aaacaggtga gagcaaaaaa aaagaaggga taagaaaaga atttttcttt
     7561 tctgtaagca atcttgaaat aaaaaaaact attatcagag tagaaaaaat agaaaacaat
     7621 ctgtagtaga aaaaatggaa agtatatcta ccacataaag aaaaaatata aataatccac
     7681 catatcatag gaggatgttc ataatatcca aagtcaaaaa ttttacctgt tgttataaaa
     7741 tatgcctcat cacctgttaa aggaaaaaaa taggaaagga aaagttttaa aattaaagta
     7801 acagaaaaaa tccagagaaa agaacaatcc tttttatttt ctaccaccag atctcctcgt
     7861 cattctttac gtttattgaa tcaagataat tttgaaggat tatctgtgct gatagtttat
     7921 ctatatattt tttccttttt tttctgctta aatctgcttc aagtaaaaat ttctctgcaa
     7981 gggaagttgt caatctctca tcaaatgata taaactcaat gtctttgatt gcttctttta
     8041 atcttgaaat aaatgcaagt gtcttttctg tttgaacact gaatgaacca tccattttta
     8101 aagggagccc ataaacaatt cttccaatat tgtatttctt tataatttcc tttatccttg
     8161 caacgatatc atcattcatt tttaaactgc caagcccttt tgccagaata ttttcttcat
     8221 cgcttatagc aatcccaata tttttatctc caacatcaat acataatatt ctcatttttg
     8281 taagattttt aagaaaaaca cactattatt ttataccatc ttcccttttc tctcaaataa
     8341 aaagggagga cggggctcgg atagaaataa caggaataaa gggatatgca attgaaatac
     8401 tggttctaca tcctccaaat ccacatcctt ctcttacttt tactatatga gaaatattta
     8461 acctgtgttt caaaagaatt tattactttg agttttcatt tcttttaata atttttgtgc
     8521 cttttctcta acatactcat gattgtcttc ctttgctatc ttttcaatct ttgaaataat
     8581 ttcgcggtca tccttataga ttataagacc acgaattgca gataatctta ttgtgaaaca
     8641 tggatcttct aatgcttttg taagaacttt tttaagaaga tttttatctt ctgaagaaag
     8701 ggctctatgt attttctcat agggggttaa atcccactcc gcactttcaa taaatggatt
     8761 acttataaga gataattcac atattgcact atctcttaca ttatcatcag gatcatttgc
     8821 acagatagta aaatgtttta aaatccattc tttatctttt gttttaatta gttgataagt
     8881 ataaggagga tgtttataag gatacgtagg atatcctatg agtaatttta taattttttc
     8941 atccaagttt ggaagttgcc caacttcaaa acttaattca tgaaaataat acctgaaata
     9001 attctcttca tcaggcataa ctttatgact atctggttta taatcatatg ttttcaaata
     9061 ttcaatttct ctttcaaaaa tttttataag tttttgaatc catttctttt tcaaatctcc
     9121 ttcactcttt tctatatttt cctttatagt tttcagttct tcaatttttt ttaatacctc
     9181 tggcgaagga ggacctattc cattaccaaa agataaagga gtgatattca ttaaaacaat
     9241 aaataaaaaa acaataataa atctgaacat tccttttttt tgttctcata ctcgtatcat
     9301 cctcctttgt taatttatct ccaatccata tgaatatatc cttttaattt tgtatcaata
     9361 taggtagctc cttctttttt tgcagtatca tgcataaatt tccaatcatc ataatgtata
     9421 tctccatccc catcccaact tgtaatctta tatctactgc atctccatac atatgtcggc
     9481 tttcagattt tccacctacc tcttcattat gaattggatt tctataacca ctactaacag
     9541 gcataggttt gttacctaaa tcttgctaca attttataag caagtggaaa ccctctccct
     9601 gatggtgcag aaggtttttg ttgccctttt tcaccttttc gccacggaaa ccgaatatga
     9661 aaactacccc tttctactat cctaaaatca ttatcacata cccaaatctc aataaatcct
     9721 gcctctgttc ctgctgtaaa tattgatgtt gctattccat tttggtttgt ctgtgatgta
     9781 ggagtaattg agccatattg aatatcactt gtaccttcat aatccgggtc tgcaaattca
     9841 aacgataaat taaaaacacc ttcccattct tcatcttcta agtcccataa acttttatta
     9901 tatataatct gtgctgagga atccatagta atcagtatag tatcatattt ccagaattta
     9961 aatttccaac tgattgtgtg attgggtaga ggagttgaat tgtattgaat tttaaccttg
    10021 tttaatgcct ggctttctcc atctgctatt aaataatcta acggctcttc tgtctccggg
    10081 tcaagaaata tatccaaatt catctggggt ggatttatct gtacaagtgt tgtatcagat
    10141 attgtatcac tgcttgcttt aatgattgaa taaaaatata aacctttatc cggatttgat
    10201 gcatctgaac ctgaaatata tatcgtactg gcttttccct agctatctgt tattttagta
    10261 tcactttgtc ccaccattac tgaatttacc attatctgca ccatttatat tgtactcggt
    10321 ctcatctttt atactgtcat atcctttatg ccctgttatc tcaaagttta tctgtattcc
    10381 ttctattgct ggagttgttg ttgctattat ctctgcttta tgtatatcta aattgggatt
    10441 aacatttcct cctgcacata tcacacccgg acttgcactt actgatactt catatactgt
    10501 cgctttccct ttatcctctc ctccttttga tgttgttagt gtcagggtta tgtcctttat
    10561 tgtgtttgat gtttgttctc ctttaagtgt taatgtcttt ggtagttgtg atatatagca
    10621 ggtatagggg aaagattttt gagttgaacc ttcccagaga gatagtttac cagaattgca
    10681 ggataaaata acttcctcat tctgtggtaa aactccttct atctgtattg tgatatttct
    10741 tgtgtttcca agtggtatat acgcaccggg ttcttattct aatcttatat ctatatccgg
    10801 tcaaaataaa gaagtcattc taatttcttt tcaattacaa agatttgata aacttttttc
    10861 tgtcgctttt ttatattctt tgttttttac attctccttt atctctttca atgtgtcaga
    10921 aactccacca tataaaagaa ttgaacaaag aataaaaaat aagaaaaatt taatctttac
    10981 tccatctgta caaaaatatc tcatttttcc ccctcttaca cttaatttat aatttttcac
    11041 tttcagatgt caaatctttc agtaatatgg atggatattt aaaaatttac aatctttttt
    11101 ctaaatctct accacttttt caatccagta aattatatca attgcatttt taacttcttt
    11161 tacatcatga accctgaaaa tacctgctcc ttttaaataa gcatatacat ttgttgctat
    11221 tgttggagat aatctctctt caacttcaag attcagaatt ttccctataa aagattttct
    11281 gcttgttcca atcaaaattg gtctattaaa ttttttgaat ttatcaagat ttttaagtat
    11341 ttcaatatta tcacaaactc tttttccaaa accaattcca gggtctatca caactctttc
    11401 aatgtttact ttattttctt caagaatttt taatttttct tcaaaaaact cacttatttc
    11461 tcccattaca tcttcataat agggatttat ctgcatattt tcaggagtac ctttcatatg
    11521 cattaaaaca tatccacaat cagaattttt tattacatca agcagatttt tatccatgga
    11581 aaaggcagaa atatcgttta ttatctttgc gccattctca atcgcctttt ctgcaacttt
    11641 acttctaaat gtatcacatg aaatgggtat ttttaattct ttgcttaaaa ctttaattac
    11701 aggaataact ctttcaattt cttctttttc atctacctgc ttacttccag gtcttgttga
    11761 ctgtccacca atatcaataa tatctgctcc ttctttttca atttcaattc ctctttcaac
    11821 tgctttgtca agattaaaaa atttaccacc atcataaaaa gaatcaggag ttacgttcag
    11881 aattcccatt atcaggggtt tttcaatatt aaagacaaaa tcttttattt tccagttcat
    11941 tgatttttaa gaagtgcttt cctgtaatct tctgcttcaa ggttttctat ctgctcaatt
    12001 tctttttttg taaagtatct caatttccca cttaaaattc cagcaaaaat tgattttgct
    12061 gcttcttcaa caattaaact tcttgtatat gcttctttta ttgattctcc aactgcaaca
    12121 actccgtgat ttttaagaag aacaacattt acatttttta tttccttaca aactgccttt
    12181 cttatttcct caccagcagg tggcacataa tcaataacag gaacatcttt cccaagtata
    12241 gcaacataat ctgggaaaaa tggcttaaat ttaactccag cagagattaa accaactgta
    12301 attggtggat gggtatgaat aactgctcta acattctctc ttattacata tattccaaga
    12361 tgcattgaaa tttcacacgt cggtcttaaa tttccaaaaa tctcacctgt ttttaaatcc
    12421 acctttaccc attcttcttc ttttatttca tcaagaaaaa atccactcgg agaaagatat
    12481 acaaaatcac catctcttgc acttatattt ccacctggtc ctgcaacaag ccctttttca
    12541 actaattttt taccatattc agtaagtttt
//
LOCUS       DAOSAT010000054        12483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3414_length_12483_cov_4.687158, whole genome shotgun sequence.
ACCESSION   DAOSAT010000054 DAOSAT010000000
VERSION     DAOSAT010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12483
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3414_length_12483_cov_4.687158"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..530)
                     /gene="def"
                     /locus_tag="PLF90_05875"
     CDS             complement(36..530)
                     /gene="def"
                     /locus_tag="PLF90_05875"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOK56839.1"
                     /translation="MRKIRKYGEEILRKKAESVKNIDEKIVKLIKSMKETLLHFKGIG
                     LAAPQVGVSKRIFIAHDKENNNLITVINPEIIDTKGEQIDIEGCLSFPEIYFSINRNK
                     IVVIKGLNEKGKEIVLEGRDLMARCFQHEIDHLNGVLIIDYATEEEKKYYREKLKKLN
                     KNKT"
     gene            complement(532..1821)
                     /gene="thiC"
                     /locus_tag="PLF90_05880"
     CDS             complement(532..1821)
                     /gene="thiC"
                     /locus_tag="PLF90_05880"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOK56840.1"
                     /translation="MKGLEKEIAKKGIISEAVKKIAKEEGLEEEELAKKVSEGKIIIL
                     KHKDKVVGIGEGLRTKINANIGTSPDVCDIKLELEKLKIAEKYGTDTVMDLSTGGKIE
                     EIRKKILKESNVPVGTVPIYQAGIEAIKEKGGIIKMNEDKIFETIEKQAEDGVSFVTV
                     HCGVNLKTIERLKKNPRKTLVVSRGGAFLISYILANEKENPLYKNFDRLIEIAKKYDL
                     VLSLGDGLRPGGLIDSTDISQIEELLTLGELTKYAWENNVQVIIEGPGHIPINEIESN
                     VLIEKKICKGAPFYVLGPIVTDIAPGYDHITSAIGGAIAAWKGADFLCYVTPSEHLSL
                     PGVEEVKEGVIVAKIAAHIGDIGKGIKKAIEIDFSMDIARTKRDWEKEKEIAVDKEKF
                     EKLRKKFPIKEKDVCTMCGKYCAIKIVEKYLGTKVIC"
     gene            complement(1818..3473)
                     /gene="ilvD"
                     /locus_tag="PLF90_05885"
     CDS             complement(1818..3473)
                     /gene="ilvD"
                     /locus_tag="PLF90_05885"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOK56841.1"
                     /translation="MRSDNIKKGYVRAPHRSLLKAIGLTDEEIERPIIGIANSGNEII
                     PGHIHLNRITEAVKAGVRMAGGTPVEFGVIGVCDGIAMGHIGMKYSLVSRETIADSVE
                     IMAQAHQFDGIVMIASCDKIVPGMLMALLRLNIPGIFISGGPMLCGILPDGKEIDLIS
                     VFEGIGKFLNKEITEEQLKLIEERACPGAGSCAGMFTANSMNCLSEALGLALPGNGTI
                     PAVDARRIRLAKYTGMKIVELVNKNLKPLDIIKFESFKNAIVVDMAIGASTNTVLHLP
                     AIANEAGIKLDLEIFDKLSRITPNICKLSPASNQHIQNLDRAGGISAVMKELSKKGLI
                     EEGCLTVSGKKIGDIIKRAEIYDRNVIKDIKSPYMKEGGIAILKGSLAPSGAVVKQSA
                     VKENMLKFTGKAIVFDCEEDAMNAVIEGKIKEGVIVIRYEGPKGGPGMREMLSITSAI
                     SGKGLEEKVALITDGRFSGGTRGPCIGHVSPEAAEGGPIAYVKNGDIIEINIPERKIE
                     LKVSKDEMEKRKKEMKILKKELRGVLARYAKLVKSANTGGILE"
     gene            complement(3591..4793)
                     /locus_tag="PLF90_05890"
     CDS             complement(3591..4793)
                     /locus_tag="PLF90_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 repeat- and reductase domain-containing
                     protein"
                     /protein_id="HOK56842.1"
                     /translation="MEKKNLFIVLSFLAIICDYVFSENIEKIVSGKSHTLILTNEGFV
                     FAFGWNKAGQIGNGNDKNISKPVMIKLNEKEYLKDIIDIGAGDIHSIGLKKDGTVLTW
                     GGNFSGQLGNLTFENSSFPVYVRYKNGQIVKDVIKVTAGWDFSVILKKDGTVWTFGDN
                     KYGQLGNGTTENRNYAVQVKGPEGKGYLKNIIDIKAGAFHVLALAKDGTVWAWGDNEF
                     GQLGDGTYIQRIFPVKTKEIKDIEKIVCGAFHSFAIDKKGNLYGWGKNWKGQIGDGSF
                     VNKNFPVKVFGKNGDGFFSDAKEVSAGRNHTVILSKNGEVFVMGSNVFGQLGNKILKK
                     INFPISILVGEETLKNVKEISASGSYSVALLKNGTLYLWGTNEGFLDDKTKNVKIFTP
                     IVIARFAE"
     gene            complement(4855..5964)
                     /locus_tag="PLF90_05895"
     CDS             complement(4855..5964)
                     /locus_tag="PLF90_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56843.1"
                     /translation="MEIKALTPEKQICFFGYYDKKQISYNGKFFLYLKVDFKDKEPGE
                     NEKAEIWIGEIGNKKFEKLDATYAWNFQQGCMLSWLSDEEIIYNVRIEKNFLSKIYNI
                     KNGKFRIINRPVSCVYPEKKIALSLNFPRLARWRPGYGYAGVEDKYENEKWPENDGIY
                     FIDLEKNMSKLIIPLSKMLEFRNEKEISDSYGWFNHTLFNKNGTRFCFVNRWKIKLKD
                     AHKTRFITSDLEGKEVYDLIDSYLISHFDWKDENKIIVWAEIKGKKGFWLIEDKTGKV
                     ETISENMQKEDGHCSFSKNKKFILYDTYPIENYRYLKVFNIEKNKEIVIDKFYSIPEI
                     TGPIRCDLHPRWENDNKITFDSIHENYRRSYLIEI"
     gene            complement(5968..7950)
                     /locus_tag="PLF90_05900"
     CDS             complement(5968..7950)
                     /locus_tag="PLF90_05900"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOK56844.1"
                     /translation="MKKIFFIFFNLILLNYSENWWNTEWKYRKKVSFNFPEEELPYKK
                     VGVINFTGNCKDNGEDIRVIDEEGKEVEYCIVSSRKPKYQILIPLDKKMYYIYYGNPK
                     SQKKNYNFNPQCGLILEIYERKDDKVSDWNESKKTLEKSIKEGKLIGKSIWKKIWDAT
                     NPFGYQKDVIKIYTGYFYIEKEGNYEFATSSSGPSFLFIDDKLVANWPGWHPARPFIT
                     PEQSGFVNLSKGIHKLIYYHFGGYNYEICVAVFKSYDKNEFEVISEKFFIPIFEGELL
                     ESEKLDGKINIDFEWENINFLRRENWNLITYKFIAKNYSGEDISYEWDFGDGQKKTGK
                     EVFHTYIGEENYNVNLTVKNKKGEVETVSYTINVEQDYSKIYLNPRKTEEYIDEFVNF
                     NLKTLPSEKLKALAEIFLSYDRLENAFNCYKELRERTLSTIDKRTINLITLKLAKKIG
                     KFEESEKICEEILQEEYNFEIFLSLIDTYIEMNKLEEGMKNCQNILNEKNLKEEEKKK
                     IELRIADILRIKGEKEKATEIYRKYTDIAEYKLKNYVYYQTVLYYLKNKDILTGIELL
                     DKWAEEFPLCKIEGKWSVLKTKIYIFKNDYINALREIEIFIKIADNENIFLPEALYLA
                     YQISKKINIKEKIEYYKERILKEFPDTEFAKELKNK"
     gene            complement(7947..8852)
                     /locus_tag="PLF90_05905"
     CDS             complement(7947..8852)
                     /locus_tag="PLF90_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56845.1"
                     /translation="MKIIIINFIFFISILFCENFEFTLSSVKNVFYEEEEVILNLKIK
                     NLSKENEEIKVQYLQPKEKNFLFVGENWSLEVISNNKKIENFTNHIPPEPKPGELNIS
                     VKPEEILNFSIPFPYYYYPLAFPLKFKVCLKYKNFSSNEIEFVVLESKGKNLKNSLIL
                     NYDFREGEEYPYGWKIGNNNVKWENKDFISFSLNKETAEGEGLWIYSIFNEIKTPSQY
                     LLSVKCKSEGPNIIIFIEGWGIVKGRKRCIERNECHIFPENKWKEYNFNIKFENENVK
                     WFRVKLYSYLNEGKVYFDRVELNKK"
     gene            complement(8849..8977)
                     /locus_tag="PLF90_05910"
     CDS             complement(8849..8977)
                     /locus_tag="PLF90_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56846.1"
                     /translation="MIKKEKIEKEKTGESISKNIYQDKIPDGYENIVLKYFSLNLK"
     gene            complement(9077..11593)
                     /locus_tag="PLF90_05915"
     CDS             complement(9077..11593)
                     /locus_tag="PLF90_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56847.1"
                     /translation="MEYTEFIFNYLNRYRNFEIKRKIKENILCSILSWLTFFLIFIVI
                     ISFYPLPVLIHFTRFFSFLLYFFFIFKIYKDIKFFKKMSFEDLAINFEKKFQEINNHL
                     INTIQIIKKSNFYPAEFIEKLKVKTFEIIKNIPPERGIGNKKIILLLKEISVVLILFI
                     LLIYLSPQKFKKWLTTIFTGFTLNYYIEPGNYEITKGESLKIKFYSKEHLPSFIEIES
                     KSTKNKFPMKNEKFYYSYFIENVDSPFNYRVINPELITPWYKVDILERTNIQKISLVL
                     KYPSYTKKSVEKKEIDFTSFEVFKNTELEMEFFFNNKVGDAYLILSNGSILTAKGTSN
                     RKKFKFTVNEPLFYKLKFYDFVTKKYLSTENEKIHIIYDNSPFIEVLEPGKDIFKKPG
                     EKFKIKIFAEDDFGIKEIRFKMNTIPNEIGKNDITFYIQKFPSESLKQTIETYLVLPL
                     KFEKPIYYYIECIDNSQPVNNKGVSSIYYIFPYTKGIEGNTLNNYLSKKEVENEKLTN
                     FLEKLAEKLKEFIEKENEVIKGEKRLENIKKITEEKDLNNLIEVQSDYFKTFQKMVND
                     LNKISKQTEGKFTLSDELVEMISHIQKSIENLKKEAIHMAISESQMGLELAKEITSNL
                     ERWLAGSPDNIKWDLQEPSKDYKVPEPALPDELEDIIGELIEKEEEMKEEIEDITSSW
                     MDSIDKGAGWGVSDGPISNMSAKGITGNLMPNQQEIGGRSGEGRTGRSYGEMVEKTAT
                     GKGGRQTPARLTPDNFEPGEIQDKSKENPLGPTGGGKTSGWGPSGLTGFEGDTTFRYN
                     QLLEKQKEIIDKAEKILLELKSTNIYNPQLEKAISQMKNF"
     gene            complement(11597..>12483)
                     /locus_tag="PLF90_05920"
     CDS             complement(11597..>12483)
                     /locus_tag="PLF90_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56848.1"
                     /translation="LKGIKNSPLENVIPVKIQGNENILIEEEFIPILTTSGKNLFIFD
                     EFMNFPPLFTINNIKYITDDSIPIFIKKDGNILMAYKLCNKGSSLIIGTDSLWKWYIS
                     GYKNKFETLFNGIIRFLIPPEKYLKLKTQTPEFKQERNIYKKGEMIEIETFFENNEIK
                     EKTNFNLITPTGEKETLKLEENKIKFRTDKEGVYIIEGGNGIYKNFIHVYVNSKGIEL
                     LNCIPDYQYLKTISEFSGGKFIPLEEVKNFEKYIPLSTKSENLKFKVTKENRKALIFV
                     LYITLNYSWFLRRKRGII"
BASE COUNT         3726 a         2157 c         1283 g         5317 t
ORIGIN      
        1 cggttcaaat ccggacaccc cgaatttttt taatttcagg ttttattttt attcaacttt
       61 ttaagttttt ctctatagta ttttttttct tcttctgtag catagtcaat aataagaact
      121 ccattcagat ggtcaatttc gtgctggaaa catcttgcca ttaaatctct tccttctaat
      181 acaatttctt ttcctttttc atttaatcct ttaataacta caattttatt tctatttatt
      241 gaaaagtaga tttcaggaaa acttaaacaa ccttctatgt caatttgttc tcctttggta
      301 tcaattattt ctggatttat aactgttatt aagttattgt tttctttgtc gtgtgctata
      361 aaaattcttt ttgatacccc aacttgagga gcagcgagtc caattccttt gaaatgtaaa
      421 agagtttctt tcattgattt tattaattta acgatttttt catctatgtt ttttactgat
      481 tctgcctttt tccttaaaat ttcttcaccg tattttctta tttttctcat tttaacatat
      541 cacttttgta ccaagatatt tttcaacaat ttttattgca caatattttc cacacatcgt
      601 acatacatct ttttctttga taggaaattt ttttcttaat ttttcaaatt tttctttatc
      661 aactgctatt tccttctctt tttcccagtc tcttttagtt cttgctatat ccattgaaaa
      721 atcaatttca atcgcttttt taataccctt ccctatatct cctatatgag cagcaatttt
      781 agcaacaata actccttctt ttacctcttc aaccccgggc aatgatagat gttcactcgg
      841 agtaacataa caaaggaaat ccgctccttt ccatgcagca attgcacctc caattgcaga
      901 agtgatatgg tcataacccg gagcaatgtc agttacaatt ggtccgagga cataaaaagg
      961 agcaccttta catatctttt tctctattaa aacattactt tcaatttcat ttattggtat
     1021 atgcccaggt ccttcaatta taacctgtac attattttcc catgcatatt ttgttaattc
     1081 tccaagagtc agtaattctt caatttgaga aatgtctgtt gaatcaataa gtcctcccgg
     1141 tcttaatccg tctcctaaac ttaaaacaag gtcgtatttt tttgcaattt ctattaatct
     1201 atcaaagttt ttatataatg gattttcttt ttcatttgca agtatatatg aaataagaaa
     1261 agcccctcct ctgcttacaa caagtgtttt tcttggattt tttttaagtc gttcaattgt
     1321 ctttaaattt actccacaat gaactgtaac aaaacttact ccatcttctg cttgtttttc
     1381 tattgtttca aaaattttat cctcattcat ctttattatt ccaccttttt cctttatcgc
     1441 ttctatccct gcctgataaa tcggaactgt tcccactggg acattacttt cttttaaaat
     1501 tttttttctt atttcttcaa tttttcctcc tgtgcttaaa tccataactg tatctgttcc
     1561 atatttttca gcaatcttta atttttctaa ctcaagtttt atgtcacaga catcgggaga
     1621 tgtcccaata ttcgcattta tttttgttct taatccttcc ccaataccaa caactttatc
     1681 tttatgtttt aaaatgatga ttttcccttc acttactttt tttgcaagtt cttcttcttc
     1741 aagtccttcc tcttttgcta tctttttaac tgcctcactt ataattccct tttttgcaat
     1801 ttctttttca agtcctttca ttcaagtatc cctcctgtat ttgccgattt aacaagtttt
     1861 gcatatcttg caagcactcc tcttaattct ttttttaaaa ttttcatttc tttttttctc
     1921 ttttccattt catctttact tacctttaat tctatttttc tttcaggtat atttatttca
     1981 ataatatctc cgttttttac ataagcaatc ggacctcctt ctgctgcttc aggacttaca
     2041 tgtccaatac agggtcctct tgtacctcct gaaaatcttc catctgtgat taaagcaact
     2101 ttttcttcta acccttttcc gcttattgct gaagttattg aaagcatttc tctcattcct
     2161 ggaccacctt taggaccttc atatcttata actatcacac cttcttttat tttaccttca
     2221 attactgcat tcatagcatc ttcttcacaa tcaaaaacaa ttgcttttcc tgtaaacttg
     2281 agcatatttt ctttcaccgc tgattgttta actactgcac cggagggagc aagagaacct
     2341 ttaagaatag caattccacc ttctttcata taaggacttt taatatcttt tataacattt
     2401 ctgtcatata tttctgctct ttttattata tcaccaattt ttttaccact tactgtcaaa
     2461 caaccttctt caatcaatcc ctttttactt agttctttca taactgctga gattccgccc
     2521 gctctatcaa gattttggat atgctgattt gatgcaggag agagtttaca aatattaggg
     2581 gtaattctgc ttaatttatc aaaaatttca aggtcaagtt taattcctgc ttcatttgca
     2641 attgcgggta gatgaagaac tgtattagtg gaggctccaa tagccatatc aactacaatt
     2701 gcatttttaa aactttcaaa ttttataatg tcaaggggtt ttaggttctt attgacaagt
     2761 tcaacaattt tcatgcctgt atattttgct aatcttattc ttcttgcatc aacagctggt
     2821 attgttccat ttccaggtaa agcaagtcca agtgcttccg ataaacagtt cattgaattt
     2881 gctgtaaaca ttcctgcaca acttcctgct ccaggacatg ctctttcctc aattaatttc
     2941 agttgttctt ctgttatttc tttattcaaa aattttccaa taccttcaaa aacacttatt
     3001 aaatcaattt cttttccatc aggtagaatt ccgcataaca taggcccacc actaataaaa
     3061 attcctggaa tatttaatct taataaagcc attaacattc cgggtacaat cttatcacaa
     3121 cttgctatca taacaattcc gtcaaattga tgtgcctgtg ccattatttc aacactatca
     3181 gcaatagttt ctcttgaaac taaactgtat ttcatcccga tatgacccat tgcaattcca
     3241 tcacaaactc ctataacacc aaattcaaca ggagttccac ctgccattct tactcctgct
     3301 tttactgctt ctgtaattct attcagatgt atatgtcctg gaattatttc atttccagag
     3361 tttgcaattc ctataatcgg tctttctatc tcttcatctg ttaagccaat tgcttttaat
     3421 aaacttctgt gaggtgctct aacatatccc ttttttatat tatcacttct catttttcct
     3481 cccatttata tttttcaact ttaaaattta aaagatttac aggtatcatt ttattatata
     3541 gcaattattc caacaaataa aggggtttaa ttttgtttaa tataatttat ttattcagca
     3601 aatctggcaa taacgatagg agtgaaaatt tttacatttt ttgttttgtc atccagaaat
     3661 ccttcatttg taccccatag atataaggtt ccatttttta ataaagcaac agaataactt
     3721 cccgatgctg aaatttcctt tacatttttt aaagtttctt ctccaacaag tatacttatt
     3781 gggaaattta ttttttttaa aattttattg ccgagttgac caaaaacatt acttcccata
     3841 acaaaaactt ctccattttt actcaagata actgtatgat ttcttcctgc agaaacttct
     3901 tttgcatctg aaaaaaatcc atctccattt ttcccaaaaa cttttacagg aaaattttta
     3961 tttacaaaac ttccatcacc aatttgacct ttccagtttt taccccatcc ataaagattt
     4021 ccttttttat ctatagcaaa tgaatgaaaa gcaccacata caatcttttc tatatctttt
     4081 atttcttttg ttttaactgg aaaaattctt tgtatatatg ttccatcacc aagttgtcca
     4141 aattcattat ctccccaagc ccagacagtt ccatcttttg caagtgcaag aacatggaat
     4201 gcccctgctt ttatatctat aatatttttt aagtatccct ttccttcagg tccttttacc
     4261 tgtactgcgt aatttctgtt ttctgtagtt ccattaccaa gttgtccata tttattatct
     4321 ccaaatgtcc agacagttcc atcttttttt aaaattacag aaaaatccca gccagcagtg
     4381 actttaatta catcctttac aatttgacca tttttatatc ttacatatac aggaaaagat
     4441 gagttttcaa atgttaaatt tccgagttgc ccggaaaaat ttcctcccca tgttaaaaca
     4501 gttccatctt tttttaatcc tattgagtgt atatcacctg cacctatgtc aattatatct
     4561 ttcaaatatt ctttttcatt cagtttaatc attacaggtt tactgatgtt tttatcattt
     4621 ccatttccaa tttgtcctgc tttattccat ccaaaagcaa agacaaatcc ttcattagtt
     4681 aaaatcaaag tatgtgattt accacttaca attttttcta tattttcaga aaatacataa
     4741 tcacaaataa tcgccagaaa ggacaaaaca atgaataaat ttttcttttc catctcttaa
     4801 attagacata ttttaaatga aaaggtttag ttttgtttgt aaaatcagga agaattaaat
     4861 ttctattaaa taacttcttc tgtaattttc gtgtatagag tcaaatgtaa ttttgttgtc
     4921 attttcccat cttggatgta aatcacatct tataggtcct gttatttcag gtattgaata
     4981 aaatttatca atgactatct ctttattttt ttcaatatta aaaactttta aatatctgta
     5041 attttcgatt ggatatgtat catataaaat gaatttttta ttttttgaaa aagaacaatg
     5101 cccatcttct ttttgcatat tttcacttat tgtttcaact ttacctgttt tatcctcaat
     5161 taaccagaaa ccttttttcc cttttatttc tgcccatacg attattttat tttcatcttt
     5221 ccagtcaaaa tgagaaataa gatatgaatc aattaaatca tatacttctt tcccttctaa
     5281 atcactggtt ataaatcttg ttttatgagc gtcttttaat ttaattttcc atctatttac
     5341 aaaacaaaat cttgttccat ttttattgaa taaagtatga ttaaaccatc cataagaatc
     5401 agatatttct ttttcatttc taaattcaag catttttgaa agaggaatta ttaattttga
     5461 catatttttt tcaaggtcaa taaaataaat accgtcattt tcaggccatt tttcattttc
     5521 atatttatct tctacacctg catatccata accaggtctc catctggcaa gacggggaaa
     5581 atttaaactc agagcaattt tcttttccgg ataaacacaa cttacaggtc tatttattat
     5641 ccggaacttt ccatttttta tattataaat ttttgataaa aaattttttt ctattctcac
     5701 attatatatt atttcttcat ctgataacca tgaaagcata catccttgtt gaaaattcca
     5761 ggcatatgtt gcatcaagtt tttcaaattt tttatttcct atttctccaa tccaaatctc
     5821 tgctttctca ttttctcctg gttctttatc cttgaaatca acttttagat agagaaagaa
     5881 tttcccatta taacttatct gttttttatc gtaataacca aaaaaacata tttgtttttc
     5941 aggggttaat gcttttattt ccatttccta tttatttttt agttcttttg caaattcagt
     6001 atcaggaaat tcttttaaaa ttctttcttt ataatattca attttttcct ttatatttat
     6061 ttttttactt atctggtaag caaggtaaag tgcttctggc agaaaaatat tttcattatc
     6121 tgctattttt ataaatattt ctatttccct taaagcatta atatagtcat ttttaaaaat
     6181 atatattttt gttttaagaa cagaccattt cccttcaatt ttacataagg gaaattcttc
     6241 tgcccattta tccagtaatt caatacctgt taatatatct ttattcttaa ggtaatatag
     6301 aacagtttga tagtatacat aatttttcaa tttatactct gctatatcag tgtattttct
     6361 atagatttca gttgcttttt ccttctctcc ttttattctc aaaatatcag caatccttaa
     6421 ttctattttc ttcttttctt cttccttaag atttttttca tttaaaatat tctgacagtt
     6481 tttcatccct tcttcaagtt tattcatttc tatatatgta tctatcaatg ataaaaaaat
     6541 ctcaaaatta tattcttcct gtaaaatttc ttcacatatt ttttcacttt cttcaaattt
     6601 acctattttt tttgcaagtt ttaaagtaat caaattaatc gttcttttat ctattgttga
     6661 tagagttctt tctcttaatt ctttatagca attaaaagca ttttctaatc tgtcataaga
     6721 taaaaatatc tctgcaagtg cttttaattt ttcacttgga agagttttta aattaaagtt
     6781 tacaaattca tctatatatt cttctgtttt tcttggatta agataaattt ttgaatagtc
     6841 ctgttcaaca tttattgtat aacttacagt ttcaacctct ccttttttat ttttaacagt
     6901 tagatttaca ttataatttt cttctcctat ataagtatga aacacctctt ttcctgtttt
     6961 tttctgacca tccccaaaat cccattcata ggatatatct tctccggaat agttttttgc
     7021 aataaattta taggttatta gattccagtt ttccctcctt aaaaaattta tgttctccca
     7081 ttcaaaatca atatttattt ttccatctaa tttttcagat tctaatagtt ccccttcaaa
     7141 gataggtata aaaaattttt ctgaaattac ttcaaattca tttttatcat atgatttaaa
     7201 aactgctacg cagatttcgt aattataacc tccaaaatga tagtaaataa gtttatgaat
     7261 accttttgat aaatttacaa aacctgattg ttcaggtgta ataaatggtc ttgcaggatg
     7321 ccagccaggc caatttgcaa ctaatttatc atctatgaat aaaaatgaag gaccggatga
     7381 agatgttgca aattcatagt ttccttcttt ttctatataa aaatatcctg tatatatttt
     7441 aattacatct ttttgataac caaagggatt tgttgcatcc catatctttt tccaaatact
     7501 ttttcctatt aatttcccct cttttatgct cttttccaat gtctttttac tttcattcca
     7561 gtcagaaacc ttatcgtctt ttctttcata tatttcaaga attaatccac attgtggatt
     7621 aaaattgtag tttttctttt gactttttgg atttccataa taaatataat acattttttt
     7681 atcaagagga attaatattt gatatttggg ctttctggaa cttacaatac agtattcaac
     7741 ttcttttcct tcttcgtcaa ttactcttat gtcttctccg ttatctttac aatttcctgt
     7801 aaaatttata acacctactt ttttataagg taattcttct tctggaaagt taaaagaaac
     7861 tttttttctg tatttccatt ccgtattcca ccagttttct gaataattta gtaaaattaa
     7921 attaaaaaat ataaaaaata tttttttcat tttttattca attcaactct gtcaaaataa
     7981 acttttcctt catttaaata tgaatagagt tttactctaa accatttaac attttcgttt
     8041 tcaaacttga tattaaaatt atattctttc catttatttt caggaaaaat atgacattca
     8101 ttcctttcta tgcatctttt tcttcctttt acaattcccc atccttcaat aaaaataata
     8161 atattaggac cttcactttt acattttaca gaaagaagat attgagacgg tgttttaatt
     8221 tcattaaaaa tggaataaat ccataaacct tctccttctg cagtttcttt attaagagaa
     8281 aaactgataa aatctttatt ttcccattta acattattgt ttccgatttt ccatccatag
     8341 gggtattcct ctccttctct aaaatcataa tttaatatta aactattttt tagatttttc
     8401 cctttacttt ctaaaacgac aaactcaatt tcattagaag aaaaattttt atattttaag
     8461 catactttaa attttaaagg aaatgctaat ggataatagt aataaggaaa aggaattgaa
     8521 aagtttaata tttcttctgg ttttactgat atatttaatt ctccgggttt tggttcagga
     8581 ggaatatgat ttgtaaaatt ttcaattttt ttattatttg aaattacttc taaagaccag
     8641 ttttcaccaa caaataaaaa atttttttct tttggttgca ggtactgaac ttttatttct
     8701 tcattttctt tacttaaatt ttttatttta agatttagaa ttacttcctc ttcttcatag
     8761 aaaacatttt ttactgaaga taatgtaaat tcaaaatttt cacagaataa aatggaaatg
     8821 aaaaaaataa aatttataat tataatcttc attttaaatt aagtgaaaaa tatttaagaa
     8881 caatattttc gtatccatct ggaattttat cctggtatat atttttagaa atagattctc
     8941 ctgttttttc cttttctatt ttttcttttt taatcaattc ccttctcaca aatgagtgat
     9001 tgaattcttt caaagaagaa attatttcac tttttgtttt taataattct ttttcctttc
     9061 ctgtttttag ttaattttaa aaatttttca tctgggatat tgctttttca agttgtgggt
     9121 tatatatatt tgtagatttt aattcaagta aaattttttc cgctttatca ataatttctt
     9181 tctgtttttc taacaattgg ttatatctga atgttgtatc tccttcaaat cctgtaagtc
     9241 cagatggtcc ccatccactt gttttcccac ctcctgtagg acccagagga ttttctttac
     9301 ttttatcttg tatttctccc ggttcaaaat tatccggtgt taatcttgca ggtgtttgtc
     9361 ttcctccttt tcctgtggct gttttttcta ccatttctcc ataactcctt ccagttctac
     9421 cttcaccact tcttcctcct atttcctgtt gattgggcat taaatttcct gttattcctt
     9481 tagcactcat attgctaata ggtccatctg aaacacccca ccctgctcct ttatctatac
     9541 tgtccatcca actacttgta atatcttcaa tttcttcttt catctcttct tctttttcaa
     9601 ttaattctcc tattatatct tcaagttcat ctggtaaagc aggttcaggt accttgtaat
     9661 cttttgatgg ttcctgaagg tcccacttta tattatccgg gcttcctgca agccatcttt
     9721 caagatttga agtaatttct ttagcaagtt caagacccat ttgtgattca gaaatagcca
     9781 tgtgtattgc ttcttttttt aagttttcaa ttgacttttg aatatgggat atcatttcaa
     9841 ctaattcatc agataatgta aattttcctt ctgtctgttt actgatttta tttaagtcgt
     9901 taaccatctt ttgaaatgtt ttaaaataat cactctgtac ttcaattaaa ttatttaaat
     9961 ctttttcttc agttattttt tttatgtttt ctaatctttt ttcacctttt ataacttcat
    10021 tttctttttc aataaattct tttaattttt cagcaagttt ttctaaaaaa tttgttaatt
    10081 tttcattttc cacttctttt ttagaaagat aattgtttaa agtatttcct tctatccctt
    10141 ttgtatatgg aaaaatgtaa taaatagaag aaactccttt attattgaca ggttgtgagt
    10201 tgtcaataca ctctatgtaa taatagatag gtttttcaaa ttttaatggt aatactaaat
    10261 aagtttcaat tgtttgtttt aaactttcag atggaaattt ttgaatataa aaagtaatat
    10321 catttttccc tatttcattt ggtattgtat tcattttaaa ccttatttct tttataccaa
    10381 aatcatcttc tgcaaaaatt tttattttga atttttcacc gggtttttta aatatatctt
    10441 tacccggttc taaaacttct ataaaaggtg aattgtcata tattatatga attttttcat
    10501 tttctgtgga aagatatttt ttggtaacaa aatcatagaa ttttaacttg taaaatagtg
    10561 gttcatttac agtaaattta aacttttttc tatttgaagt tccctttgca gttaatatgg
    10621 aaccattgct taaaatcaaa tatgcatctc ctactttatt attgaaaaaa aactccattt
    10681 caagttccgt atttttaaac acttcaaaag atgtaaaatc aatttctttc ttttccactg
    10741 atttttttgt ataggaagga tatttcaata ccagtgagat tttctggata tttgttcttt
    10801 caagaatatc aactttatac catggagtta ttaattcagg atttattact ctataattaa
    10861 aaggagaatc cacattctct ataaaatatg aataataaaa tttttcattt ttcataggaa
    10921 atttattttt tgttgattta ctttcaattt ctataaaaga tggtaaatgt tctttgctat
    10981 aaaattttat ttttagtgat tcaccttttg taatttcata atttccaggt tctatataat
    11041 agtttaaagt aaaacctgta aatattgtgg ttaaccattt tttgaatttt tgcggggata
    11101 aataaatcaa taaaataaat aaaatcaata ctactgaaat ttcctttaaa agtaaaatta
    11161 tctttttatt ccctattccc ctttcaggag gtatattttt aataatttca aatgttttaa
    11221 ctttaagttt ttcaataaat tctgctggat aaaaattact ttttttaatt atctggattg
    11281 tatttataag atgattattt atctcctgaa attttttttc aaagttaatt gcaaggtctt
    11341 caaaagacat ttttttaaaa aatttgatat ccttgtaaat cttgaaaatg aaaaagaaat
    11401 ataaaaggaa ggaaaaaaat cttgtaaaat gtattaaaac aggtaatgga taaaatgaaa
    11461 ttataactat aaaaattaaa aaaaatgtta gccacgaaag tattgaacat aaaatgtttt
    11521 cttttatttt tctttttatt tcaaagtttc tatatctgtt taaataatta aaaataaatt
    11581 cagtatattc catcttttat ataatacccc tttttcttct taaaaaccaa gagtaattca
    11641 gggttatata aagaacaaaa atgagtgctt ttctattttc ttttgtaact ttaaatttta
    11701 gattttcact tttagtagat aaaggtatat atttttcaaa attttttact tcttctaaag
    11761 gtataaattt tcctccagaa aattctgaaa tagtttttaa atattgataa tcaggaatac
    11821 aatttagcaa ttctattcct ttgctgttta cataaacatg tataaagttt ttataaattc
    11881 catttccacc ctcaattata tatactcctt ctttatccgt cctgaactta attttatttt
    11941 cttctaattt tagtgtttct ttttcaccgg taggagttat aagattgaaa tttgtttttt
    12001 cttttatttc attattttca aaaaaagttt ctatttctat catctcccct tttttatata
    12061 tatttctttc ctgtttgaat tcaggagttt gtgtttttaa ttttaaatat ttttcagggg
    12121 gtattaaaaa tcttataatt ccattaaaaa gagtttcaaa tttattttta tatccagaaa
    12181 tataccattt ccataaacta tcagttccaa tgattaaaga acttccttta ttgcacaatt
    12241 tatatgccat aagaatattg ccatcttttt ttataaaaat tggaattgaa tcatctgtaa
    12301 tatattttat gttgtttata gtaaagagag gaggaaagtt cataaattca tcaaaaataa
    12361 aaagattttt cccagaagta gttaaaatcg ggatgaactc ttcttcaatt aatatatttt
    12421 cattaccctg gattttaacc ggtattacat tttctaatgg tgagtttttt atacctttta
    12481 gaa
//
LOCUS       DAOSAT010000055        11965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3589_length_11965_cov_4.994458, whole genome shotgun sequence.
ACCESSION   DAOSAT010000055 DAOSAT010000000
VERSION     DAOSAT010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11965
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3589_length_11965_cov_4.994458"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLF90_05925"
     CDS             complement(<1..339)
                     /locus_tag="PLF90_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56849.1"
                     /translation="MGKINVNGVFPNLAVKAKHLPARTETGIGALMPWADKLWFITYV
                     SHKKNTGDGTGLFYIDDDFNLFKHPESRVGTYANRMIHHESNKLIIGPHTIDIDGKVS
                     TFEELVDIRLA"
     gene            complement(342..1577)
                     /gene="fabF"
                     /locus_tag="PLF90_05930"
     CDS             complement(342..1577)
                     /gene="fabF"
                     /locus_tag="PLF90_05930"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOK56850.1"
                     /translation="MKRVVVTGVGLVTPVGNDTKTTWENLINGVSGVDKITAFDVSSY
                     DTKIGAEVKNFTLENIHPKEKRKMDTFVQFAIKATDEAIKDSGIEFTDEEKEKVGVIV
                     GAGIGGLRVIEQQHKILLESGPDRVSPFLIPMLIPDIASGHIAILYGFKGVNFCTVSA
                     CASGAHALAISLNLIRAGICDVIIAGGTESCITPLGLAGFCSMKALSRRNDQPQKASR
                     PFDRERDGFVMGEGAGVVVLESLEHAKKRGSKIYCEFIGAGMSCDAYHITAPDAEGFG
                     AYLSMKYALEDAKINPEDVDYINAHGTSTPLNDKSETFAIKKLFKERAYKISVSSIKS
                     MIGHLLGAAGAVEFVSTCLTINTGIIPPTINYEYPDPECDLDYVPNKAREEKVKIAIS
                     NSFGFGGHNVTLVLRDFEE"
     gene            complement(1574..1819)
                     /locus_tag="PLF90_05935"
     CDS             complement(1574..1819)
                     /locus_tag="PLF90_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOK56851.1"
                     /translation="MSREEIAKTIKEIIVEKLGVNPSEVTEEAAFVEDLGTDSLDTVE
                     LVMDFEEKFGLEIPDEDAEKIKTVKDAIDYIEKKKAQ"
     gene            complement(1877..2608)
                     /gene="fabG"
                     /locus_tag="PLF90_05940"
     CDS             complement(1877..2608)
                     /gene="fabG"
                     /locus_tag="PLF90_05940"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOK56852.1"
                     /translation="MFKDKVILITGAFGGIGKSLISKFGHDKAKLVLWDIFIDEEFER
                     SLNEKGIETISKKIDITKEQEVEQGSKEIINKWSRVDVLINNAGITKDKLIFRMSEKD
                     WDDVLNVNLKGAFLCSKIIGQIMYKQKSGKIVNIASIIGEIGNIGQANYSSSKGGLIA
                     LTKTCAKEFARAGINVNAVAPGYIMTKMTENLPDKVKEEMLKLIPLGRFGRPEEVAEL
                     VYFLASEKSDYITGQVFRIDGGLVM"
     gene            complement(2601..3536)
                     /gene="fabD"
                     /locus_tag="PLF90_05945"
     CDS             complement(2601..3536)
                     /gene="fabD"
                     /locus_tag="PLF90_05945"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOK56853.1"
                     /translation="MKIGVVFPGQASQYIGMGKEILEENNKYEEILKIGERITNLPLI
                     EKIFNGPIEDLTRTLFCQPSVFGISLVCWRYFKDKAGIIPSYLAGHSLGEYTALCAGE
                     VFSIEEGFYLVKKRAEIMDEISQKVNGSLLAILGLSLNDVKGIIKNFNDIEISNINSY
                     TQIIVGGKKDELEKLEKYLKEKRIKAVFLKVSGPFHTKYMVEASEILEKEIDKINFKN
                     SKVPIYLNFSSEKTIDKEEIKNGLLKQLYSPVRWVEIIENMVKDGIECFVEIGPKNVL
                     KKLIEVIVPGIPSFNIENPEGLKVFSSFLEGGKNV"
     gene            complement(3533..4513)
                     /locus_tag="PLF90_05950"
     CDS             complement(3533..4513)
                     /locus_tag="PLF90_05950"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOK56854.1"
                     /translation="MGIGIIGTGSYLPELKLTNKDMEMMVDTSDEWIISRTGIRERRI
                     CPKGYASSDLALEASLRAIEDAEIKKEEIEFIICATITPDMYFPSTACIVQKKLGLEN
                     IPSFDFQAACSGFIYGIEIAKSLIIGSGYKKGLVIASECMSRLTDYTDRSTCVLLGDG
                     AGAAILSETEEEGIIDSYLSSNGSYENLLFTPGGGSLNPSSFKTIEKRENFMKMEGST
                     LFKIAIYSMSESVIKVLERNNLKKEDISIVIPHQANLRIIAGVAKNLDIPIEKFFINI
                     EKYGNMSAACVPIALDEASKKGKIKKGDLVVTVAFGAGLTWAANLIRWIK"
     gene            complement(4498..5490)
                     /gene="plsX"
                     /locus_tag="PLF90_05955"
     CDS             complement(4498..5490)
                     /gene="plsX"
                     /locus_tag="PLF90_05955"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOK56855.1"
                     /translation="MRQIRIALDAMGGDNAPISPAKNCEFIKDDKLKIYLIGKEEKIR
                     QVLKSSIDIVEIIDCQDFVAMDEKVTSTLLKRNTSMKVAIQMQKERKVDISLSAGNTA
                     AFVSLAISELGMIKGIERPSIAVLFPNITGGVTIFMDAGANISPKPLHLFQSGIMGSL
                     YSKNVFNINNPKVALLNVGEEETKGDDLRKQAYKMLKESDKINFIGNIEGHEIFSGKA
                     DVIITDGFTGNIVLKTSEGITRSFRTMLMKEIMKNIQGKIGTLLISKNLKSFARKADY
                     AEYGGGILLGVNGNVIISHGRSSPRAIYSAIKLGEKIINSKLLEEMKELKWESE"
     gene            complement(5487..5672)
                     /gene="rpmF"
                     /locus_tag="PLF90_05960"
     CDS             complement(5487..5672)
                     /gene="rpmF"
                     /locus_tag="PLF90_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOK56856.1"
                     /translation="MPNPKRKHSKSRTGKRRNSKKVRSVPLTKCPNCGAVKLPHFVCN
                     VCGYYKGVQVIKVETSK"
     gene            complement(5638..6159)
                     /gene="coaD"
                     /locus_tag="PLF90_05965"
     CDS             complement(5638..6159)
                     /gene="coaD"
                     /locus_tag="PLF90_05965"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOK56857.1"
                     /translation="MKKIGVYPGTFDPITYGHIDVVERSLKIVDKVIIAVSGFQRKNF
                     LFSVDERVELIKGVFKSNKSVKVESFDGLLVDYLKERNINIIIRGLRAISDFDYEFQM
                     VLINRKLNRNVETIFLMPREEYFYISSSIVKEIAKLNGNISCFVPKNVEIALRKKFKE
                     VKNAESEKKTLKK"
     gene            complement(6156..6707)
                     /gene="rsmD"
                     /locus_tag="PLF90_05970"
     CDS             complement(6156..6707)
                     /gene="rsmD"
                     /locus_tag="PLF90_05970"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOK56858.1"
                     /translation="MRVISGLYKGRKLKFIKDKEIRPTKDIVREAIFDTLRGWIVNKK
                     VLEMFAGSGILGIEAISNGAKKIIFIEKDKRGIKVIKENIESLGIEKKCEVIKGDCEY
                     EIEKLQNMKYDLIIGDPPYEFPISKLERIMQKILELNILKKNGIMVIEHQFKREMPVI
                     EGYEIIKKRKYGKSCLTYFRRIK"
     gene            complement(6704..7591)
                     /gene="secF"
                     /locus_tag="PLF90_05975"
     CDS             complement(6704..7591)
                     /gene="secF"
                     /locus_tag="PLF90_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOK56859.1"
                     /translation="MEIIKNTNFDFLGKRKYFYIFSSLIIITGIVYMIIRGEKNFGVD
                     FVGGDLINIEFNQKMDVVNLRKTIADTNVGSFTVQELGTEDKNFIIRLPQNTSDMVIE
                     KLKEKFGEDFIIKGKSIVSPSMSVSLRKKSLKAFFIGLIGILIYLTIRFEFKFAVGAT
                     IAIFHDILIVLSILSLSGKQIDGMVIAALLTIVGYSVNDTVVIFDRIRENLRKTRSTD
                     YIGIFNKSINETLSRTILTVLTTLFVVLSIFFFGGETLHTFSFCLLIGFIFGTYSSIF
                     IASAIVIDWVKRSSSRLKL"
     gene            complement(7593..8912)
                     /gene="secD"
                     /locus_tag="PLF90_05980"
     CDS             complement(7593..8912)
                     /gene="secD"
                     /locus_tag="PLF90_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOK56860.1"
                     /translation="MKTQFYIKFVIILAVIGFCFYLFYPPEKKLKKGLDLQGGIHVVL
                     EVEKEKIEEKSLSDLTERALEIIRNRIDALGVTEPVIQKEGTYRIIVDLPGVKDPEKA
                     IEIIGKTALLEFKLVIDDENLFKQALQGNVPDGYELLYLKEKDERGIYRETQPLLLKK
                     EPELTGKYIVDAYVGYDRTGFPDVNLVLDKEGAKIFEEVTAKNVGKRLAIVLDGVIKS
                     APQIRERIPEGKAVITGKFNMEEARELAIVLRSGALPAKLNVIYQEVVGPTLGKEYIQ
                     KGFRASLYGAIIVVVFMMFYYLHFGLLADFALALNILILLGIMAGIKGVLTLPGIAGI
                     ALTCGMAVDANVLIFERIREELRAGKSPKASVDAGYHRAWSAIIDSNITTLITGLVLF
                     LFVEGGIKGFGLTLSIGIIANLFTAVYVTKVIVDWVIINRKIEKLRI"
     gene            complement(8909..9286)
                     /gene="hisI"
                     /locus_tag="PLF90_05985"
     CDS             complement(8909..9286)
                     /gene="hisI"
                     /locus_tag="PLF90_05985"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOK56861.1"
                     /translation="MEKILNVVKFNEQGLIVSVVQDLNGNILMVAWMNKEAIEMTLKT
                     GKMHFYSRSRKKLWLKGEESGNFQYVKEIYIDCDGDTLLFKVEQKGGACHEGYYTCFF
                     RKYEDGEFKIVKEKIFEPKEVYR"
     gene            9331..9597
                     /locus_tag="PLF90_05990"
     CDS             9331..9597
                     /locus_tag="PLF90_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOK56862.1"
                     /translation="MRNKNTIKKKLWQILLKIPIGETVSYSEISKKINMPKKTRYIST
                     LLKENPYPVSIPCHRVIYKNGKSGKYIFGEEFKKYIIEWEKLFK"
     gene            complement(9586..10221)
                     /locus_tag="PLF90_05995"
     CDS             complement(9586..10221)
                     /locus_tag="PLF90_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56863.1"
                     /translation="MWDLSQKTHREEIALRIANGIENIIPLIEKDINYNPDFSYIYRR
                     IKVSRYMITEDVLNGSLNEAEKYYKEYERLKKELDENPDRKKEKRWYIEITKNYRRAQ
                     WYLSVKDRYEIQKSEPKVEIDIHILRIGGIVFATNPFECYLDYGMRIKARSKAIQTFI
                     VQLVGGGTYLPTERAVKGGSYGAIAPSCVVSPEGGKELVDETVKCIEELFK"
     gene            complement(10360..11052)
                     /locus_tag="PLF90_06000"
     CDS             complement(10360..11052)
                     /locus_tag="PLF90_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56864.1"
                     /translation="MGKLFIGWKEVDITPEQPVCLCGQFHARISEGIKDPITATVLIL
                     ESIKEKESSECVIMISCDLVAIPYEFKKVVVEEIKKILSDIDETKIIINATHTHTAPE
                     PGGDIFRGRDRSDLSKCYGIDLPVMSPNDFIKFASEKIKEGVKIAWENRKEGGIAYGL
                     GFAVVGHNRRISYYDGHSQMYGRTDVPDFSHVEGYEDHSINLMATYDKDKNLTGLVVN
                     IACPSQVSENEF"
     gene            complement(11068..>11965)
                     /locus_tag="PLF90_06005"
     CDS             complement(11068..>11965)
                     /locus_tag="PLF90_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOK56865.1"
                     /translation="KGIIVVHLHGLPVEMDKVIKIAKKYNLKVIEDACQAHGAKYKGK
                     KVGSFGDCTAFSFNQNKCLCSGEGGMFVTDDSEIYERAKMLWSFGETRRPFENRDYHA
                     YALGWMYRNNDLTAAFGRAQLKKLDWYLEVQKKNADVLNRLLSEVEGLILPYVPENCE
                     HNWYNYTIRLDMEYFGIKKDVRKFRDYVLKAIQLEGVKCGIWQSFILPAMTVFQAKNA
                     YGKGCPWSCQFYGKEMRYIPEDYPEAQKHCDTHFGMTEPLRAPNDEKVAELVAIAIKK
                     VIDDVKEKGSVKWMNKVEEVLK"
BASE COUNT         3714 a         2463 c         1289 g         4499 t
ORIGIN      
        1 tgcaagtcgt atatctacaa gttcttcaaa agtagaaacc ttaccatcta tatcaattgt
       61 gtgaggacct attattaatt tatttgattc atgatgtatc attctgttag catatgttcc
      121 aactctgctt tcaggatgtt taaaaagatt aaaatcatcg tcaatgtaaa ataatcctgt
      181 accatctcct gtattttttt tatgagagac atatgtaata aaccataatt tatcagccca
      241 cggcattaaa gcaccaatac ctgtttctgt ccttgcgggt aaatgttttg cttttactgc
      301 aagatttgga aagacaccat taacattaat tttccccatt tctattcctc aaaatccctc
      361 aaaacaagag taacattatg tccaccaaaa ccaaaagaat ttgatattgc aattttaact
      421 ttttcttctc ttgctttatt tggaacatag tcaaggtcac actcaggatc tggatattca
      481 taattaatgg tcggaggtat tatacctgta tttatagtaa gacaggttga tacaaattct
      541 actgctcctg ctgcaccaag aagatgccca atcattgatt ttattgaact tactgaaatt
      601 ttatatgctc tttctttaaa taatttttta attgcaaatg tttcactttt atcatttaaa
      661 ggagtgcttg ttccatgagc atttatataa tcaacatctt ctggattgat ttttgcatct
      721 tcaagagcat atttcattga aagataggca ccaaaacctt ctgcatcagg agcggttata
      781 tgatatgcgt cacaactcat tccggcacct ataaactcac agtaaatttt acttcctctt
      841 tttttagcat gttccagtga ttcaagaaca actactcctg ctccttcacc cattacaaat
      901 ccatctcttt ccctatcaaa tggccttgac gctttctgtg gctggtcatt tcttcttgaa
      961 agtgctttca ttgagcaaaa accagcaagt ccaaggggtg taatacagga ttcagttcca
     1021 cctgcaatta ttacatcaca tattcctgcc cttattaaat tcagagaaat tgcaagagca
     1081 tgagcacctg aagcacaggc acttacggta cagaaattca ctcctttaaa accataaaga
     1141 attgctatgt gtcctgatgc aatatcagga attaacattg gaatgagaaa aggagaaacc
     1201 ctgtcaggac cactttcaag taaaatctta tgctgctgtt ctattactct caagccacct
     1261 attccagcac ctactataac tccaactttt tctttttctt cgtctgtaaa ttcaattcca
     1321 ctatctttaa ttgcttcatc tgttgcttta atggcaaatt gaacaaaagt atccatcttt
     1381 cttttttctt ttggatgaat attttcaaga gtgaagttct taacttctgc acctattttt
     1441 gtatcataag aagaaacgtc aaaagcagta attttatcaa cccctgaaac accatttatg
     1501 agattttccc atgttgtttt agtatcgttt ccaacaggtg taacaagacc aactccggtt
     1561 accactactc ttttcattgc gccttctttt tctctatata atcaattgca tcctttactg
     1621 tttttatttt ttctgcatct tcatctggaa tttcaagtcc aaatttttct tcaaaatcca
     1681 taacaagttc aactgtatca agagagtcag taccaaggtc ttctacaaat gctgcttctt
     1741 ctgtaacctc tgaagggtta actcctaatt tttcaactat gatttccttt attgtttttg
     1801 ctatttcttc tctactcata ttctcctcct ttcttttaag tttatttaat tatttttttc
     1861 aattttttta atcctattac attacaagtc caccgtctat tctgaaaacc tggcctgtaa
     1921 tataatctga cttttcagac gccagaaaat atacaagttc tgcaacttct tcaggtctac
     1981 caaatcttcc aagaggtatc agttttaaca tttcttcctt tactttatcc ggtaaatttt
     2041 cggtcatttt tgtcattata tatccgggag caaccgcgtt aacatttata cctgcccttg
     2101 caaattcttt tgcacatgtt ttcgtcaaag caataagtcc accttttgaa gaagaataat
     2161 ttgcctgtcc aatatttcca atttctccta ttattgatgc tatattaact attttaccac
     2221 tcttttgttt atacatgatt tgacctatta ttttactaca taaaaaagcg cctttcaagt
     2281 tcacatttaa aacatcatcc cagtcctttt cactcattct aaaaataagt ttatcttttg
     2341 taataccagc attatttata agaacatcca ctctactcca cttattaata atttctttac
     2401 ttccctgttc aacttcctgc tcctttgtga tatctatttt ttttgaaatt gtttctatcc
     2461 ctttttcatt taaagacctt tcaaactctt catctataaa aatatcccac agtacaagtt
     2521 ttgctttatc atgtccaaac ttgcttataa gtgatttccc aattccaccg aaggctcctg
     2581 ttattaaaat aactttatcc ttaaacattt tttcctcctt ctaaaaaaga actaaaaact
     2641 ttaagacctt ctggattttc aatattaaaa gagggaatac ccgggacaat cacctctatt
     2701 aattttttta aaacattttt aggccctatt tccacaaaac attcaatccc atccttaacc
     2761 atattttcaa ttatttcaac ccatcttaca ggactgtaaa gttgcttcaa taagccattt
     2821 tttatttctt ctttatcaat tgttttttca cttgaaaaat tcaggtatat tggaacttta
     2881 gagtttttaa aatttatttt atctatttct ttttccaata tttcagaagc ttccaccata
     2941 tattttgtat gaaatggacc actgactttt aaaaaaaccg cttttattct tttctccttc
     3001 agatattttt ccagtttctc aagttcatct tttttccctc caacaataat ttgggtataa
     3061 gaatttatat tggaaatttc aatgtcatta aaattcttaa taatcccttt tacatcgttt
     3121 aaagaaagac ccaaaattgc aagcaatgaa ccatttacct tttgtgaaat ttcatccatt
     3181 atttctgctc tctttttaac aagataaaaa ccttcttcaa tggaaaaaac ctctcctgca
     3241 cacaaagcag tatactcacc taaactatgt cctgctaaat aagaaggaat tattcctgct
     3301 ttatctttaa aatatctcca gcaaaccaaa ctgataccaa aaacagatgg ttgacagaat
     3361 aaagttcttg tcaaatcttc aattggacca ttaaatattt tttctataag aggcaaattt
     3421 gttattcttt ctccaatttt aagtatttct tcatatttat tattttcttc cagaatttct
     3481 tttcccattc caatatattg agatgcctgt cctggaaaaa ccacccctat tttcatttta
     3541 tccaccttat taaattggct gcccatgtaa gacctgctcc aaaagcaaca gtaactacaa
     3601 gatctccttt ttttattttc cccttttttg atgcctcatc aagagcaata ggaacacagg
     3661 cagcagacat atttccgtat ttttcaatat taataaaaaa tttttcaatt ggaatatcaa
     3721 gatttttagc aactcctgct ataatcctca aatttgcctg atgaggtata acaattgaga
     3781 tatcttcctt tttcaaatta tttctttcca gaacttttat cactgattca ctcatggaat
     3841 aaatagcaat tttaaagagt gtagaacctt ccattttcat aaaattttct cttttttcaa
     3901 tagttttaaa acttgaagga tttaaagaac cacctcctgg tgtaaacaaa agattttcat
     3961 aactaccatt tgaagaaaga tatgaatcaa ttatcccctc ttcttctgtt tcacttaaaa
     4021 ttgccgctcc tgctccatca ccgagtaaaa cacatgtact tctatctgta taatcagtaa
     4081 gccggctcat acattcagat gcaatgacca atcccttttt ataaccagac ccaattatta
     4141 aactttttgc tatttctatt ccatatataa aacctgaaca tgctgcctga aaatcaaaag
     4201 aagggatatt ttcaagtcca agttttttct gaactataca cgctgttgat ggaaaataca
     4261 tatccggtgt tattgtagca cagataataa actcaatctc ttcttttttt atttcagcat
     4321 cctctatcgc tcttaaagat gcttccagtg ccagatctga tgaagcataa cctttaggac
     4381 agattcttct ttctctaatc cctgttcttg aaattatcca ttcatcactt gtatcaacca
     4441 tcatttccat atctttattt gtcaatttca attctggtaa atacgaaccc gtcccgatta
     4501 ttccgattcc cattttaact ccttcatctc ttcaagtaat tttgaattaa taattttttc
     4561 tccaagttta attgcactgt aaattgctct tggagaactc cttccatgac ttattatcac
     4621 attcccatta actccaagca aaattcctcc tccatactct gcgtagtctg cttttcttgc
     4681 aaaacttttt aaatttttac ttattaataa agttcctatt ttcccctgta tatttttcat
     4741 tatctctttc ataagcatgg ttctaaaaga ccttgttatt ccttcagatg ttttcaagac
     4801 aatattccct gtaaaaccat ctgttataat cacatccgct ttccccgaaa atatttcatg
     4861 tccttctata tttcctataa aattaatttt atcactctct tttaacattt tatacgcctg
     4921 ttttcttaaa tcatctcctt ttgtttcttc ttctccaaca tttaaaagtg caacttttgg
     4981 attatttata ttaaatacat tttttgaata aagagacccc attataccag attgaaatag
     5041 atgtaaaggt tttggactta tgtttgcacc cgcatccata aaaatagtaa cacctcccgt
     5101 aatattagga aacaatacag caattgatgg tctttcaatt ccctttatca taccaagttc
     5161 tgaaatagcg agtgaaacaa aagcagcggt attccctgca ctgagtgaaa tatcaacttt
     5221 cctttctttt tgcatctgta tcgccacttt catacttgta tttcttttta aaagagttga
     5281 agtaactttt tcatccattg caacaaagtc ctgacagtca ataatttcaa ctatatcaat
     5341 actacttttt aaaacctgcc ttattttttc ttcttttcct attaaataaa tttttaattt
     5401 atcatctttt ataaactcac aatttttggc aggtgaaata ggtgcattat ctccacccat
     5461 cgcatcaaga gctattctta tctgtctcat ttggaagttt ctactttaat aacttgaact
     5521 cctttataat acccacagac attacagaca aaatgaggaa gttttacagc cccacaatta
     5581 ggacatttag ttaatggaac acttcttact tttttactat ttcttctttt ccctgttcta
     5641 ctttttgagt gttttctttt cggattcggc atttttaacc tccttgaatt tttttctcaa
     5701 tgcaatttca acatttttcg gaacaaaaca ggaaatattc ccatttaatt tagcaatttc
     5761 ttttactatt gaagaactta tataaaaata ttcctctctg ggcattaaaa atattgtttc
     5821 aacatttcta ttcaattttc tattaattaa aaccatttga aattcataat caaaatcact
     5881 gatagccctc aaccctctta taattatatt gatatttctt tcttttaaat aatcaacaag
     5941 aagcccgtca aaactttcca ctttcactga tttattactt ttaaaaactc ctttaattaa
     6001 ttcaactctt tcatcaactg aaaaaagaaa attttttctt tgaaatccac taacagcaat
     6061 aataacttta tcaacaatct ttaaacttct ttcaacaaca tctatatgac cataagttat
     6121 tgggtcaaaa gtcccgggat aaacacctat ttttttcatt taattctcct gaaataggtt
     6181 aaacatgatt tcccatattt tctttttttt ataatttcat aaccttcaat aacaggcatt
     6241 tctcttttaa attgatgttc aattaccata atcccgtttt ttttcagtat attcaattca
     6301 agtattttct gcataatcct ttcaagtttt gaaattggaa attcgtatgg gggatcccca
     6361 ataattaagt catatttcat attttggagt ttttctattt catactcaca atcccctttt
     6421 ataacctcgc attttttttc aatacctaaa ctttctatat tttcttttat cacttttatt
     6481 cctcttttgt ccttttcaat aaaaataatt ttttttgctc cgttgcttat tgcttcaatt
     6541 ccaagtattc cacttcctgc aaacatttca agaacttttt tgttaacaat ccatcctctt
     6601 aaagtatcaa aaattgcttc tctaacgata tcctttgttg gccttatttc tttatctttt
     6661 ataaatttta attttcttcc tttataaagc ccacttataa ctctcataat ttaagtctgg
     6721 aggaacttct tttaacccag tcaataacta ttgcagaggc aataaatata gaagaatatg
     6781 tcccaaatat aaatccaatt aaaagacaga aagaaaatgt atgtaatgtt tctcctccaa
     6841 aaaagaaaat agaaagaact acaaaaagag tagttaatac agttaaaata gttctgctta
     6901 atgtctcatt tatactttta ttaaaaattc ctatataatc agtacttctg gtcttcctta
     6961 aattttctct gattctatca aatatcacaa ctgtatcatt tactgaataa cctacaattg
     7021 ttagtaaggc ggctataacc ataccgtcta tctgttttcc acttaaagat aaaattgaaa
     7081 gtactattaa aatatcatgg aatattgcta ttgttgcacc aacagcaaat ttaaattcaa
     7141 atcttatggt cagatatatt aatattccaa taagtcctat aaaaaatgct tttaatgatt
     7201 tttttcttaa actcacactc attgaaggag atacaatgga tttccctttt attatgaaat
     7261 cttcaccaaa tttttctttc aatttttcta taaccatatc agaagtattc tggggtaatc
     7321 taataataaa atttttatcc tctgttccca gttcctgaac agtaaaagaa ccaacattag
     7381 tgtctgcaat agtttttctt aaatttacaa catccatttt ttgattaaat tcaatattta
     7441 tcagatctcc accaacaaaa tcaacaccaa aatttttctc acctcgtata atcatataga
     7501 caatcccggt aattattatt aaagatgaaa aaatatagaa atattttctt tttcccaaaa
     7561 aatcaaaatt tgtgtttttt ataatttcca ttttatatcc tcaacttttc tatttttcta
     7621 tttattataa cccagtcaac tatcactttt gttacataaa cagcagtaaa taaatttgct
     7681 attataccta tactcaaagt taaaccaaaa ccctttatcc cgccttctac aaaaagaaaa
     7741 agaacaagcc cggttatcag agttgttata tttgaatcaa ttattgcact ccatgctcta
     7801 tggtatcctg catctacact tgccttggga gattttcctg ctctcaattc ttcccttatt
     7861 ctctcaaaaa tcaaaacatt tgcatcaact gccataccac atgttaaagc aataccagca
     7921 atacccggca aagtcaaaac cccttttata ccagccatta ttccaagtaa aattaaaata
     7981 ttcaaagcaa gggcaaaatc agccagaaga ccaaagtgaa gataataaaa catcataaat
     8041 actacgacaa ttattgcccc ataaagggac gctctaaaac ccttctgtat atattccttt
     8101 ccaagagtag gcccaactac ttcctgataa ataacattta actttgccgg taaagcacct
     8161 gaccttaaaa caattgcaag ttcccttgcc tcttccatat taaatttacc tgttataact
     8221 gctttacctt ctggtatcct ttctcttatt tgaggagcac tttttataac cccatcaagt
     8281 acaatagcaa gtcgttttcc aacatttttg gctgttactt cttcaaaaat tttagcacct
     8341 tctttatcaa gaactaaatt tacatctgga aaaccagttc tatcatatcc aacataagca
     8401 tctactatat atttacctgt taattcaggt tctttcttca aaagcaatgg ttgcgtctct
     8461 ctataaatcc ctctttcatc cttttctttt aaataaagta attcatatcc atctggaaca
     8521 tttccctgca atgcctgttt aaaaagattt tcatcatcta taacaagttt aaactcaaga
     8581 agtgctgtct taccaatgat ctctattgct ttttccgggt ctttaacacc gggtaaatca
     8641 actattattc tatatgttcc ttctttctga attaccggct ctgttactcc aagagcgtca
     8701 atcctatttc ttataatttc aagtgctctt tcagttaaat cagaaagaga tttttcttca
     8761 attttttctt tttctacttc aagaacaaca tgaattccac cttgcaaatc aagacccttt
     8821 ttcaattttt tttcaggtgg ataaaacaga taaaaacaaa aacctatcac tgccaaaata
     8881 ataacaaact taatataaaa ttgtgttttc atctgtatac ctcttttggt tcaaaaattt
     8941 tttctttaac aattttaaat tcgccatctt catattttct aaaaaaacat gtataatatc
     9001 cttcgtgaca cgcaccacct ttttgttcca ctttaaaaag aagtgtatct ccatcgcaat
     9061 caatataaat ttctttcaca tattgaaaat tgcctgattc ttcacctttc aaccataatt
     9121 ttttcctgct tctactgtaa aagtgcattt ttcctgtttt taaagtcatt tcaattgctt
     9181 ctttattcat ccatgcaacc atcaaaatat ttccatttaa atcctgaact acacttacaa
     9241 ttaatccctg ttcattaaac ttcactacat tcaaaatctt ttccatttta tttaaaaatt
     9301 ttataatatt tttaaaaaca atacaaaaaa atgagaaata agaataccat aaagaaaaaa
     9361 ttatggcaaa ttttattaaa aattcccatc ggagaaacag taagttattc agaaataagt
     9421 aagaaaatca atatgcctaa aaaaacaaga tatatttcaa ctctgttaaa agaaaatccc
     9481 tatccagttt caatcccctg tcatagagta atttacaaaa atgggaaatc tggaaaatat
     9541 atatttggtg aggagtttaa aaaatacata attgaatggg aaaagttatt taaataattc
     9601 ttctatacac tttactgtct catctacaag ttcctttcct ccttcaggac taactacaca
     9661 tgaaggagca atcgctccgt aactgcctcc ctttactgct ctttcagtcg gaagatatgt
     9721 tcctcctcct acaagttgaa caataaatgt ctgaattgct ttacttcttg ctttaattct
     9781 cattccataa tcaagataac actcaaaagg atttgtagca aaaacaatac caccaattct
     9841 taaaatgtgg atatcaatct caacttttgg ttctgatttc tgaatttcat atctatcttt
     9901 aacacttaaa taccactgcg ctctccgata gttttttgta atttctatat accatctttt
     9961 ttcttttttt ctgtctggat tttcatccag ttcttttttc aatctttcat attctttata
    10021 atatttttct gcttcattca gggacccatt taaaacatcc tcagtaatca tgtatcttga
    10081 aactttaatt cttctgtaaa tatatgaaaa atcaggatta tagttaatat ctttttcaat
    10141 aagaggaata atattttcaa taccgttagc aattcgcaaa gcaatttctt ccctatgagt
    10201 tttttgtgat aaatcccaca ttctctgttc actcttttta tttataagga tatgcgggga
    10261 ttggtctcct gctgcactgc actgagatag aatacaaatg ttttctccaa atctttttct
    10321 taattctttt cttgtctcat gccagaaatc agcacttatt taaaattcat tctcactgac
    10381 ctgcgaagga caggcaatat tcacaacgag acctgttaaa tttttatctt tgtcataggt
    10441 tgccattaaa tttattgaat ggtcttcata tccttcaaca tgactgaaat caggtacatc
    10501 tgttcttccg tacatttgag aatgcccatc ataatatgaa atccttctgt tatgtccaac
    10561 aactgcaaat cccaagccat aagcaattcc accttctttt ctgttttccc atgcaatttt
    10621 cacaccttct tttatttttt cagaagcaaa ttttataaaa tcattcggac tcatcacagg
    10681 taaatctatt ccataacatt tagacaaatc gctcctatcc ctacctctaa aaatatcacc
    10741 tccgggttca ggtgctgtat gagtatgggt agcatttatt ataatttttg tttcatctat
    10801 atccgacaaa atttttttta tttcttctac aactactttt ttaaattcat acggaattgc
    10861 aactaaatca caacttatca taataacaca ttctgaactt tctttttctt taattgactc
    10921 aagaattagt acagttgcgg ttattgggtc ttttatccct tctgaaattc ttgcatgaaa
    10981 ctgtccgcag agacatacag gttgttcagg agttatatca acctctttcc atccaataaa
    11041 taattttccc attctattct cctttttcta ttttaaaact tcttcaactt tattcatcca
    11101 ttttacagat cctttttcct taacatcgtc aattactttt tttattgcta tggcaacaag
    11161 ttctgccact ttttcatcat ttggagccct taatggttct gtcattccaa aatgtgtatc
    11221 acaatgtttt tgtgcttcag gataatcttc aggaatatat ctcatttctt taccataaaa
    11281 ctggcaactc caaggacaac cttttccata agcatttttc gcctgaaaaa cagtcattgc
    11341 tggtagtata aagctctgcc atataccaca tttaacccct tccaattgaa ttgctttcaa
    11401 aacataatct ctgaatttcc tgacatcctt tttaattccg aaatactcca tatccaatct
    11461 tattgtataa ttataccagt tatgctcaca gttttcggga acatatggaa gaattaaacc
    11521 ctctacttca cttaataatc tattcaaaac atcagcattt tttttctgaa cttcaagata
    11581 ccagtcaagt ttttttaatt gtgctctacc aaaagcagcg gttaaatcat tatttctgta
    11641 catccaacca agagcatatg catgataatc tctgttttca aatggtcttc ttgtctctcc
    11701 aaaactccac aacattttag ccctttcata tatctcactg tcatctgtta caaacattcc
    11761 accctctcct gaacacaaac atttattttg attgaaactg aaagcagtac aatctccaaa
    11821 acttccaact tttttacctt tatattttgc accatgtgct tgacatgcat cttcaataac
    11881 ttttaaattg tatttttttg ctatttttat aactttatcc atttctacag gtaatccgtg
    11941 aagatgaacg actataattc ctttt
//
LOCUS       DAOSAT010000056        11892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3616_length_11892_cov_5.837205, whole genome shotgun sequence.
ACCESSION   DAOSAT010000056 DAOSAT010000000
VERSION     DAOSAT010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11892
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3616_length_11892_cov_5.837205"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..944
                     /locus_tag="PLF90_06010"
     CDS             24..944
                     /locus_tag="PLF90_06010"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOK56866.1"
                     /translation="MGIKKLKVELGKIKLDSPIIMASGTFGYGTEKLDFIDFEKIGAI
                     TTKTITYQPWSGNPQPRIYETYSGMINNIGLQNPGVFYFTKNILPFIRKKFKRVFVSI
                     TGRSEKEFIDIIKILNKENIDAIELNLSCPNFEKNQKMISQDANLTHKLIKKIKNETD
                     IPLIAKLSPNVTDITEIAIAAQEGGVDILALINTVRGLGVDIKKMRVINGGLSGSSIK
                     PIGLKCVYEVYKVVDIPIIGIGEITEGRDAVEYFLSGASAVGIGSGLFTNTKIIDEVI
                     ESLREFLKKTGKENLKEITGLLNEKKGNHK"
     gene            919..2706
                     /locus_tag="PLF90_06015"
     CDS             919..2706
                     /locus_tag="PLF90_06015"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOK56867.1"
                     /translation="MKKKEIINKIYCYILSSLIFCRFLFDGITYPKFNLFFTISIFLL
                     FIVFTLLNFQKLEFTYVEVLILLFSFFSIISSFLSEIKGTGIRYNSYIVSYLCLFFLV
                     RNIFRDKEKSVLIYIILFTVFTLTLYGIYQRFWGIEATRKYLQSQESLEQNPEILKYL
                     SPTFFDRMESNRIYSTFVYPNIYASFLISIMPFLFFLFLNEDKKYFKIFSVLLFLLCF
                     INLIFTESMGGLLIFLFISHIILLQLLLNEKKFKRILTFLIFFEFLFLYLGYHFKVLP
                     HIHSLLDRITYWKGSMKIIQKNIFMGVGPENFRYYFLKYKSPEGLEAAHAHNFLIETF
                     AENGFIGVIFLMSFLFIIILNPLKERKKDFLSNGVFYLLLTFFLHNLIDFDFSDPSVA
                     ILFFIFGGFYEYKDKFKIKGLTKPLLCFIIILYAFNIFELSKFENSERYRRYSEIEEN
                     LDVKLYLLDYAENQYNKNFEVYFEKGNLYTYMWRLTEKDEFFEQAILNYEKSIHLNPY
                     LVKSYRRLAYIYESKGNYEKAEEMYLKVLEKYPNKKLYNLEIAKFYKKIGNEDKFKYY
                     FQKAKQLKEVTIEEKLIIEEVEKWIELQK"
     gene            2685..3350
                     /gene="ricT"
                     /locus_tag="PLF90_06020"
     CDS             2685..3350
                     /gene="ricT"
                     /locus_tag="PLF90_06020"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOK56868.1"
                     /translation="MDRIAEVEIRKVEKNLYFKLHSEVDVKVKDFVLVEYTQGTVDYG
                     KIYKIFEPKTIFSPTISGKVLRKLNEKDFIIIQENNEKRKEAFNICLEKVKSYNLPMR
                     LIDADFSYDKTKVTFYYWADGRIDFRKLVRDLAKIFNCRIEMRQIGLRDEAKIKGGYG
                     ICGRELCCITFLKQFESITMRMVKNQKLPLDMNKITGLCGRLLCCLNYEDEFYKKEKV
                     EKK"
     gene            3347..4828
                     /gene="metG"
                     /locus_tag="PLF90_06025"
     CDS             3347..4828
                     /gene="metG"
                     /locus_tag="PLF90_06025"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOK56869.1"
                     /translation="MKFYITTPLYYVNAEPHIGHTYTTVAADCLARFYRMKGFDVFFL
                     TGTDEHGQKIYETSKKEGLTPIELADRVSETFKNMWKKMNISYDRFIRTTEKQHEEVV
                     KKVFKKLLEKGDLYKGEYRGYYCIHCESYVNVENENQPLCPDCKRPVSEISESSYFFK
                     LSKYQKLLENMIETTEFVKPDFRKNEVLNFIKNGLSDISITRKNVLWGIESPTEEKYT
                     IYVWFDALLNYLSGIGYLYDETTFKKYWPADVQLIGKDIMRFHHIIWPAILFALELPQ
                     PKKIFAHGWWISGKEKISKSKGNIISPEYFIKEYGVDPFRFFLLREVPFGLDGEFSEE
                     NFKKRYNSDLVNDFGNLLNRSLNLIEKKFGNYIPEGEPDREIMEISEITYKNFSEKLE
                     NISFSEGIEEIWKLVVFLNKYLDEKAPWRDSSDSEKTIISIFYGIRNLLIFLYPFIPQ
                     TCEKMIEMLGLNKKEIEKFDNLKIKYQGRVKIGKREIVFYRKK"
     gene            4825..5559
                     /gene="pyrH"
                     /locus_tag="PLF90_06030"
     CDS             4825..5559
                     /gene="pyrH"
                     /locus_tag="PLF90_06030"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOK56870.1"
                     /translation="MKPVFKRILLKLSGEALQENNKGGISSEALISISKEIKDVWELG
                     CEIGLVVGGGNIYRGSQRDKEVIESVTADYMGMLATVINGLALQNALEKLGLDTRVQS
                     AFEMRQFCEPYIRRRAIRHLEKGRIVIFVGGTGNPYFTTDTAAALKAVEIKADVVLKA
                     TKVDGVYSADPFKYPDAKRYKNLSYLEIIQKQLKIMDAAAISMCMENKIPIIVFNLFK
                     KGNLKKIVLGKGVKTIISEKGGLNEK"
     gene            5549..6106
                     /gene="frr"
                     /locus_tag="PLF90_06035"
     CDS             5549..6106
                     /gene="frr"
                     /locus_tag="PLF90_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOK56871.1"
                     /translation="MRSKIYNETEQKMKGVVDFFTKEISKMRAGRASISLLEGVLVEC
                     YGSKMPINQVATITIPQPQMIVIQPWDKSIYGNILKAIQTNNLGLTPISDGNIIRVPI
                     PPLSEERRKELVKILHNMGEEAKVEIRELRRKANEQLKKAKEDKQISEDDMFKGTEEI
                     QKLTDKYIEEVDKKIKDKEKEIMEE"
     gene            6107..6793
                     /gene="cmk"
                     /locus_tag="PLF90_06040"
     CDS             6107..6793
                     /gene="cmk"
                     /locus_tag="PLF90_06040"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOK56872.1"
                     /translation="MKNFVIAIDGPAGAGKSTVAKIIAKKLNFLYVDTGAMYRALTLK
                     AMKEKIPWEDTEKIVEMAKNTKIELYNDSDRYKVFVDGEDVSDEIRKEDVSKNTSYIA
                     SMLKIREILWDMQRKYREKNNIVMEGRDIGSKVFPDAQLKIYLDASVDERAKRRYLQL
                     KQMGIEEDIEKIKKEIIERDEKDKNRKIAPLVKQDDYFYIDTTNLTIDEVVQKIISLY
                     ETKKQLSMYQ"
     gene            6908..8467
                     /gene="cimA"
                     /locus_tag="PLF90_06045"
     CDS             6908..8467
                     /gene="cimA"
                     /locus_tag="PLF90_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOK56873.1"
                     /translation="MEKNKIEVYDTTLRDGAQGENVFFTLNDKIKIAEKLDEFGIDFI
                     EGGWPGSNPKDIAFFKKIKNKKFKNSKIVAFGSTRRKNYPVEKDPFIKKLLESETEYI
                     TIFGKSWDLHVADVLKITLDENLKLIEETVDFLTKHGRKVFFDAEHFFDGYKRNPEYA
                     LKTLLVAENYGVERIVLCDTNGGSLPFEVEEIVKKIKEKIKIPLGIHTHNDSSLGVAN
                     TVSAVKSGAVHIQGTINGFGERCGNADLTAIIPILQIKMGYKCIPDENLIHLTELSRF
                     IYEVANIVPPNNQPFVGLSAFAHKGGVHIDAVSKNPLTYEHIDPKVVGNERRILISEL
                     SGKSTILQKFKDYKLEEKPEIIKKLVDMVGELENEGYQFEAGEGTFELMVRKALGKYK
                     KLFEVEGFRVIVEKKGKKVISEATVKVKVGKLIEHTASEGNGPVNALDNALRKALEKF
                     YPEIKEMKLTDYKVRILKPEKATAAVTRVLIESTDEKDTWGTVGVSENIIEASWKALL
                     DSFEYKILKFR"
     gene            complement(8469..9659)
                     /locus_tag="PLF90_06050"
     CDS             complement(8469..9659)
                     /locus_tag="PLF90_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56874.1"
                     /translation="MLKDIKQLIDIACYYPEEIERVLKGDSFLKFDPELGYIQINYIF
                     RDGIYNTLSEYTYDNKYNYRKIVNYPEKPCRINTYGNSYTQCAQVSDGETWQEILAAH
                     FREPIRNLGVGGYGVYQAYLRMMRIEKTDIGTEYIILNIWDDDHLRNIDSSRWIRVAW
                     MHRDLPRGGGENTYPVHGFPWAHLRFDIEKERFIEIPGRCKNEDDLMKLTDREYYYNF
                     FKDDHVVHLFALTQGLDAPIDYLEKLGESFGLKIDLRNKKKRIKDAQKLHITYGIKST
                     IYILEKLKKWIADNKKKLMVILSYDAPTCKKYIKKGERFDEELLEYLKTNNFCFIDTL
                     EKTKEEYKIYKLSVDDFLSRLYIARAGAQVFGHYSPSGNFWFAWTIREELVKFLDPKP
                     PAYR"
     gene            9750..>11892
                     /gene="uvrA"
                     /locus_tag="PLF90_06055"
     CDS             9750..>11892
                     /gene="uvrA"
                     /locus_tag="PLF90_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOK56875.1"
                     /translation="MEPKYISIKGAREHNLKNIDLDLPRNKLIVITGISGSGKSSLAF
                     DTLYAEGQRRYIESLSAYARQFLEQMKKPDVEQITGLPPAIAIEQRKAAANPRSTVAT
                     TTEIYDYLRVLFARVGIPHCPNCGRVISRQSSSEIIDRIMNFENTSEIKVLAPVIRGR
                     KGEYRKIFDQIKTMGFLKVRVDKKYYDVDEEIKLDRYKIHNIEVLIDNYRVKDEDKTR
                     IAESVEMALKIGKGIVIVEKDGKDIIFNENYSCPICGISFEEISPRMFSFNSPYGACP
                     ECKGLGFLMKVDPDLVIPDKTKTFREGAIKPWHDVGGRGLFWYYRSLLNDILDSLGRS
                     IDDRVCDLSKRELDIVIYGSEEYNFEGVIPNLERLFHQTESEYRREEILKYIREVTCP
                     SCKGGRLRPESLAVKIAGKSIMDICKMSVVKAKEFFENLKLSEKEQIIAKQILKEILS
                     RLNFLEEVGLDYLTLDRMTHTLSGGEAERIRLATQIGSGLVGVLYILDEPTIGLHQRD
                     NLKLINTLKHLRDIGNTVVVIEHDEETIKNADFIVDLGPGAGIHGGEVVAKGTLEDII
                     KNENSITGKYLSGKLKIEIPTKRRKPGEKKIKILKASHNNLKNIDVEIPLGLFVCVTG
                     VSGSGKSSLIEDVLYKGLKKILYDSKEEPGKHDAILGTEYINKVVVIDQSPIGRTPRS
                     NPATYTGAFTYIREIFANTYEAKIRGYTPGRF"
BASE COUNT         4541 a         1384 c         2078 g         3889 t
ORIGIN      
        1 gtttttgatg gtaaaaaaat aatgtgggaa taaaaaaatt aaaagtagaa cttggaaaga
       61 taaaacttga ttctccaatt ataatggcaa gtggaacatt tggatacggt actgaaaaac
      121 ttgactttat tgattttgaa aagataggtg ccattacaac aaaaacaatt acttatcaac
      181 catggagtgg aaatcctcaa cccagaatat atgaaacata ttcagggatg ataaataata
      241 tagggcttca aaatccagga gttttttatt ttaccaaaaa cattcttcct tttataagaa
      301 aaaaatttaa aagagttttt gtgagtatta caggaagaag tgagaaagaa ttcattgata
      361 ttattaaaat tttaaataaa gaaaatattg atgcaataga attaaatctt tcctgtccaa
      421 attttgaaaa aaatcagaaa atgatttctc aggatgcgaa tttaacccat aaacttataa
      481 aaaaaataaa aaatgaaaca gatattcctc ttatagcaaa actttcacct aatgttactg
      541 acattacaga aattgcaatt gctgcccaag aaggaggagt tgacatactt gctcttatta
      601 acactgtaag gggtttaggt gttgatatta aaaaaatgag agtaattaat ggagggttat
      661 caggttcatc tataaaacca attggattaa aatgtgttta cgaagtttat aaagttgttg
      721 acattccaat tattggtatt ggtgaaatta cagaagggag ggatgcagtt gaatattttc
      781 tgtcaggagc atcagccgtt ggtattggaa gcggtttgtt cacaaataca aagattatag
      841 atgaagttat tgaaagtttg agagaattct taaagaaaac agggaaagaa aacttaaaag
      901 aaattacagg tttattaaat gaaaaaaaag gaaatcataa ataaaatata ctgttatatt
      961 ctatcttctc ttatattttg taggttcctt tttgatggta ttacttatcc aaaattcaat
     1021 cttttcttta caatttcaat ttttctttta tttattgtct tcactttact aaattttcaa
     1081 aaacttgaat ttacttatgt tgaagtttta attttacttt tttcattttt ttcaataatt
     1141 tcgtctttcc tttcagaaat aaaaggaaca ggtataagat ataattcata tattgtctca
     1201 tatctgtgtc tattttttct ggtgcgaaat atatttaggg ataaagaaaa gagcgtttta
     1261 atttatatta ttttgtttac tgtttttact ttaactttat acggaattta tcaaagattc
     1321 tggggtattg aagcaacaag aaaatatctt caaagtcagg aaagtttaga acaaaatcct
     1381 gaaattttaa aatatctctc tccaactttt tttgatagaa tggagagtaa tagaatttat
     1441 tcaacatttg tttatcctaa tatttacgct tcttttctta tttcaattat gccatttctg
     1501 ttctttcttt ttctaaatga ggataaaaaa tactttaaaa ttttttctgt tcttcttttt
     1561 cttctgtgtt ttataaattt aatttttaca gaatctatgg gtggcctttt aattttcctt
     1621 tttatttccc atatcatttt attacaactt ttacttaacg aaaaaaagtt taaaagaata
     1681 ctaacttttc ttatattttt tgaattttta tttttatatc ttggatatca ttttaaagtt
     1741 cttcctcata tacattcttt acttgataga ataacttact ggaagggtag tatgaaaatt
     1801 attcaaaaaa atatttttat gggagtaggt cctgagaatt tcagatatta ttttttaaaa
     1861 tataaatcac ctgagggtct tgaagcagca catgctcata attttttaat agaaacattt
     1921 gctgaaaatg gatttatagg agtgattttt ttaatgtcat tcttgtttat tataatatta
     1981 aatcctttaa aagagagaaa aaaggatttt ttaagcaacg gagtttttta tcttctactt
     2041 actttttttc ttcataattt aattgatttt gatttttctg acccatcagt tgcaatttta
     2101 tttttcattt ttggtggttt ttatgaatat aaagataaat ttaaaattaa aggattgaca
     2161 aaacctttat tatgttttat tattatttta tatgcattta acatttttga actctcaaaa
     2221 tttgaaaatt cagagagata tagaagatat agtgaaatag aagaaaatct ggatgtaaag
     2281 ttatatttac ttgattatgc agaaaatcag tataataaaa actttgaagt ttactttgaa
     2341 aagggaaatt tatacacata tatgtggcgg ttaacagaaa aagatgaatt ttttgaacaa
     2401 gcaatattaa attatgaaaa atcaattcat ctaaatccgt atttagtaaa aagttataga
     2461 cgtcttgctt atatttatga atctaaagga aattatgaga aagcagaaga aatgtatctg
     2521 aaagttttgg aaaaatatcc gaataaaaaa ttatataatc ttgaaatcgc aaaattttat
     2581 aaaaaaatag gtaatgaaga taagtttaaa tattactttc aaaaagcaaa acaactaaaa
     2641 gaagttacaa ttgaagaaaa acttataatt gaagaggttg aaaaatggat agaattgcag
     2701 aagtagaaat cagaaaggtg gaaaaaaatc tgtattttaa gttgcatagt gaagtggatg
     2761 ttaaagtaaa ggattttgtt ctggtggagt atactcaggg aacagtagat tacggtaaaa
     2821 tttataagat ttttgaacct aaaacaattt tttcaccaac aatttcaggc aaagtattga
     2881 gaaaattaaa tgagaaagat tttattataa ttcaggaaaa taatgaaaaa agaaaagaag
     2941 catttaatat atgtctggaa aaagtaaaaa gttataatct gccaatgcga ttgattgatg
     3001 ctgatttttc ttatgataaa actaaagtta ctttttatta ctgggcagat ggaagaattg
     3061 atttcaggaa acttgtaaga gaccttgcta aaatttttaa ttgcagaatt gaaatgagac
     3121 agattggttt aagagatgaa gcaaaaatta aaggaggata tggaatttgt ggaagagaat
     3181 tgtgttgtat tactttttta aaacagtttg aatcaattac gatgagaatg gttaaaaatc
     3241 aaaaactacc acttgatatg aataaaataa cagggttgtg tggaagattg ttatgctgtt
     3301 taaattacga agatgaattt tataaaaagg aaaaggtaga aaaaaaatga aattttatat
     3361 aacaactcca ttatactatg ttaatgcaga accacacata ggacatacct atacaacagt
     3421 agcggcagat tgtcttgcaa ggttttatag aatgaaggga tttgatgttt tttttcttac
     3481 aggaacggat gaacacggac agaaaattta tgaaacttca aaaaaagaag gattgacacc
     3541 tattgaactt gctgatagag tttcagaaac atttaaaaat atgtggaaaa aaatgaacat
     3601 ttcttatgat agatttataa gaaccacgga aaagcaacat gaagaagttg taaaaaaagt
     3661 ttttaaaaaa cttttagaaa agggagacct ttataaaggt gaatacagag ggtattactg
     3721 tattcactgt gaaagttatg ttaatgttga aaatgaaaac cagcctttat gtcctgattg
     3781 taaaagaccc gtatctgaaa tttctgaatc ttcttatttt tttaaacttt caaaatatca
     3841 gaaattgctt gaaaatatga ttgagaccac cgaatttgtt aaacctgatt tcagaaaaaa
     3901 tgaagttctt aacttcataa aaaatggact atcagatata agtataacaa gaaaaaatgt
     3961 tttatggggt atagaatcac caactgaaga aaaatataca atttatgtat ggtttgatgc
     4021 tcttttaaac tatttaagcg gaattggata tctctatgat gagacaacat tcaaaaaata
     4081 ctggccagcg gatgtccagt taattggtaa agatattatg agatttcatc atatcatatg
     4141 gcctgccatt ttgtttgctc ttgaattacc ccaaccaaaa aaaatatttg ctcacggatg
     4201 gtggatttct ggaaaagaaa aaatttcaaa gtcaaagggt aatattattt ctcctgaata
     4261 ttttattaaa gaatatggtg ttgacccctt tagatttttt cttttaagag aggttccttt
     4321 tggtcttgat ggagagtttt cggaagaaaa ttttaaaaaa agatataata gcgaccttgt
     4381 taatgacttt ggaaatttac tgaacaggag tttaaatctt attgaaaaaa aatttggaaa
     4441 ttatattcct gagggagaac ctgacaggga gattatggaa atttcagaaa taacttataa
     4501 aaatttttct gaaaaacttg aaaatatttc tttttcagaa ggtattgaag aaatttggaa
     4561 acttgttgta tttttaaata agtatcttga tgaaaaagca ccttggagag atagttctga
     4621 tagtgaaaag acgattattt caatttttta tggcataaga aatcttttaa tctttttata
     4681 tccttttata ccacaaacat gtgaaaaaat gatagaaatg cttggtttga ataaaaagga
     4741 aattgaaaaa tttgacaatt taaaaattaa atatcaaggg cgagttaaaa ttgggaaaag
     4801 agaaatagtc ttttatagaa aaaaatgaaa ccagttttta aaagaatttt attaaaactt
     4861 tcaggtgaag cactgcagga aaataacaaa ggtggtatta gttcagaagc actgatttca
     4921 atttctaaag aaataaaaga tgtctgggaa ctgggatgtg aaattgggct tgttgtcgga
     4981 ggaggtaata tttatagagg cagtcaaaga gataaagaag ttattgagtc tgttactgct
     5041 gattatatgg gaatgcttgc aactgttata aatgggcttg ctttacagaa tgcacttgaa
     5101 aaactcggtc ttgatacaag ggttcagagt gcttttgaaa tgcgtcaatt ctgtgaacca
     5161 tatataagaa ggagagcaat aagacatctt gaaaagggaa gaattgtaat atttgttggt
     5221 ggcacaggaa atccatattt taccactgat actgcggcag cactaaaagc agtagagata
     5281 aaagcagatg ttgttttaaa agcgactaaa gttgatggtg tttattctgc agaccctttt
     5341 aaataccctg atgcaaaaag atataaaaac ttaagttatc tggaaataat acagaaacaa
     5401 ctgaaaataa tggatgctgc agcaatatcc atgtgtatgg aaaataaaat tccaataatt
     5461 gttttcaatc tttttaaaaa gggaaatttg aagaagattg ttcttggaaa aggagtaaaa
     5521 acaattattt cagagaaagg aggactaaat gagaagtaag atttataatg aaacagaaca
     5581 aaaaatgaaa ggggtagtgg atttttttac taaagaaatt tcaaagatga gagcaggcag
     5641 ggcttcaata tctctacttg aaggagtttt agttgaatgt tatggttcaa aaatgccaat
     5701 caatcaggtt gctacaatta caataccaca gccacagatg atagtcattc aaccatggga
     5761 taaaagtatt tatggaaata ttttaaaagc aatacagaca aataatcttg gacttacacc
     5821 tatatcagat ggaaatataa tcagagttcc gatccctcct ttaagcgaag agagaagaaa
     5881 agaacttgta aaaattttac ataatatggg agaagaggca aaagttgaaa taagagaatt
     5941 aagaagaaag gcaaatgaac agttaaaaaa agcaaaagag gataaacaga tttctgaaga
     6001 tgatatgttt aaaggaactg aagaaattca aaagttgaca gataaatata tagaggaagt
     6061 agacaaaaaa ataaaagata aagaaaaaga aataatggaa gaataaatga aaaactttgt
     6121 tatagcaatt gatggtccag caggagcagg gaaaagtact gttgcaaaaa ttattgctaa
     6181 aaaattgaat tttctttatg ttgatacagg tgcaatgtat agagcactta ctttaaaagc
     6241 aatgaaagaa aaaataccgt gggaagatac agaaaaaatt gttgaaatgg caaaaaatac
     6301 aaaaattgaa ctttataatg attctgatag atataaagtt tttgttgatg gtgaagatgt
     6361 aagtgatgaa ataagaaaag aagatgtgag taaaaatact tcttatattg cttcaatgct
     6421 gaaaataaga gagattttat gggatatgca aagaaaatac agagaaaaaa ataatattgt
     6481 tatggaaggg agagatatag gaagtaaagt gtttccagat gcacagttaa aaatatatct
     6541 ggatgcctca gttgatgaaa gggcaaaaag aagatattta caattaaaac agatgggaat
     6601 tgaagaggat attgaaaaga taaaaaagga aataattgaa agagatgaaa aagataaaaa
     6661 cagaaaaatt gctcctcttg tcaaacagga tgattatttt tacatagata ccacaaatct
     6721 tacaattgat gaagttgtcc agaaaatcat ttctctttat gaaacaaaaa aacagttgtc
     6781 aatgtatcaa taacttttat ttgaaaattt agtatgaata aaatcttatt aagataattt
     6841 tcttttcaat ttttttactt ttatttcaaa cttctgtata atattattat taggagagaa
     6901 aaacaaaatg gaaaaaaata agatagaggt ttatgatact acactaaggg atggagcaca
     6961 gggagaaaac gtttttttta ctcttaatga taaaattaag attgcagaaa aacttgatga
     7021 atttggtatt gattttatag agggtggctg gcctggttca aatcctaaag acattgcttt
     7081 tttcaaaaaa ataaaaaata aaaaatttaa aaatagtaaa attgttgcct ttggaagtac
     7141 aagaagaaaa aattatcctg ttgaaaaaga cccttttata aagaaattac ttgaaagtga
     7201 aacagaatat ataacaattt tcggaaaatc gtgggattta catgttgctg atgttttaaa
     7261 aattacactt gatgaaaatt taaaacttat tgaagaaaca gtagattttt taactaaaca
     7321 tggcagaaaa gtattttttg atgctgagca tttttttgat ggatataaaa gaaatcctga
     7381 atatgcatta aaaacacttc ttgttgctga aaattatgga gtagaaagaa ttgttttatg
     7441 tgatacaaat ggaggaagtt taccatttga agttgaagaa attgttaaaa aaattaaaga
     7501 gaaaattaaa atacctcttg gaattcatac tcataacgat tcttctcttg gagttgcaaa
     7561 tactgtaagt gctgttaaaa gtggagcagt tcatattcag gggacaataa atggttttgg
     7621 agaaaggtgt ggaaatgctg acttgaccgc aataattcca attttacaga ttaaaatggg
     7681 ttataaatgt attcctgatg aaaacttaat tcatctaacg gaactttcaa gatttattta
     7741 tgaagtcgca aatattgttc ctccaaataa tcaacctttt gttggtttaa gcgcttttgc
     7801 ccataaagga ggggttcaca ttgatgcagt tagtaaaaat cctttgactt atgaacatat
     7861 tgacccaaaa gttgttggga atgaaagaag aattttaata tctgaacttt ccggtaaaag
     7921 cacaatttta cagaaattta aggactacaa acttgaagaa aaacctgaaa ttataaagaa
     7981 acttgttgat atggttggag aacttgaaaa tgaaggttat cagtttgaag caggtgaagg
     8041 gacttttgaa cttatggtaa gaaaagcact cggcaaatat aagaaacttt ttgaggtgga
     8101 aggttttaga gttattgttg agaaaaaagg taaaaaagta atttctgaag caactgttaa
     8161 agttaaagtt ggtaaactta ttgaacatac agcaagtgaa ggaaatggac ctgtaaatgc
     8221 acttgataat gccttgagaa aagcacttga aaaattctat ccagaaatta aagaaatgaa
     8281 actgactgat tataaggtaa gaatactgaa acctgagaaa gcaactgctg ctgttacaag
     8341 agttttaata gaatcaacag atgagaaaga tacatgggga acagttggcg tctcagaaaa
     8401 cataattgaa gcatcatgga aagcacttct tgacagtttt gaatataaga ttttaaaatt
     8461 tcgctaaatt atctatatgc aggaggtttg gggtctaaaa atttaacaag ttcttctctt
     8521 attgtccatg caaaccagaa atttccagag ggagaataat gaccaaatac ttgtgctcct
     8581 gctcttgcaa tatataatct tgaaagaaaa tcatcaacac ttaatttata aattttatat
     8641 tcttcctttg ttttttcaag agtatcaata aaacaaaaat tgtttgtttt taaatattca
     8701 agcaattcct catcaaatct ttctcccttt ttaatgtatt ttttacaggt aggggcatca
     8761 taacttaaaa ttaccataag ttttttcttg ttatctgcaa tccatttttt gagtttttca
     8821 agtatatata ttgttgattt tatgccataa gttatatgta gtttctgggc atctttaatt
     8881 cttttctttt tatttcttaa atcaatcttt aatccaaagg attctccaag tttttcaaga
     8941 taatcaatag gtgcatctaa tccctgagta agagcaaata aatgtacaac atggtcgtct
     9001 ttgaaaaaat tataataata ttctctatca gttagtttca ttaaatcatc ttcattttta
     9061 catcttccag gtatttcaat aaatctttct ttttcaatat caaatcttaa gtgtgcccat
     9121 ggaaatccat gaacaggata tgtattttct ccaccacctc ttggtaaatc tctgtgcatc
     9181 catgcaactc ttatccatct agaagagtca atatttctta aatggtcatc atcccatata
     9241 tttaaaatta tgtattcagt tccaatatca gttttttcaa ttctcatcat cctcagatat
     9301 gcctgataaa caccatatcc gccaacacca agatttctta tcggctctct gaaatgtgcg
     9361 gctaatattt cctgccatgt ttcaccatct gaaacctgag cacattgagt ataactattt
     9421 ccatatgtgt ttattctaca aggtttttca ggataattta caatttttct ataattatat
     9481 ttgttatcgt atgtatattc agaaagtgta ttatagattc catctctgaa aatataatta
     9541 atttgaatat atccaagttc agggtcaaac tttaaaaacg aatctccttt taaaactctt
     9601 tcaatttctt caggataata acaagcaata tcaataagtt gctttatatc ctttaacatc
     9661 ttctttccta ttttttataa tattatttta tacataagca ttaggttgtc aattaaaaaa
     9721 agaaaaggta aaatatttat ttattacaaa tggaaccaaa atatataagt attaaaggtg
     9781 caagagaaca taatcttaaa aatatagacc ttgatttgcc aagaaacaaa cttattgtta
     9841 ttactggaat ttctggttca ggtaaatcat ctcttgcttt tgatacttta tatgctgaag
     9901 gacagaggag atatatagaa agtttatccg cttatgcaag gcaattttta gagcagatga
     9961 aaaagccaga tgttgaacag ataacaggac ttcctccagc aatagcaata gaacaaagaa
    10021 aagcagcggc aaatccaagg tctacagtgg caacaacaac tgaaatttat gattatttaa
    10081 gggtcctttt tgcaagagtt ggaattcctc actgtccaaa ttgtggaagg gtcatttcaa
    10141 gacagtcctc atcagaaatt atagacagga ttatgaattt tgagaatact tctgaaatta
    10201 aagttctcgc tcctgttata agaggaagaa agggtgaata caggaaaatt tttgaccaga
    10261 taaagacaat gggattttta aaagtaaggg ttgataaaaa atattatgat gttgatgaag
    10321 aaataaaact tgatagatat aaaattcata atatagaggt tttaattgat aattataggg
    10381 taaaagatga agataaaaca aggattgcag aaagtgttga aatggctttg aaaataggga
    10441 aggggattgt tatagttgag aaagatggca aggatataat atttaatgaa aattacagtt
    10501 gtccgatctg tggaataagt tttgaagaaa tatctccaag aatgttttca tttaacagtc
    10561 cttatggtgc atgtcctgaa tgtaagggac ttggtttttt gatgaaagtc gaccccgacc
    10621 tcgttattcc tgataaaaca aaaacattca gagaaggagc gattaaacca tggcatgatg
    10681 ttggagggag aggacttttc tggtattaca ggtctctttt aaacgatatt cttgattctc
    10741 ttggcaggag catagacgat agagtttgtg acctttcaaa aagggaactg gatatagtaa
    10801 tttatggttc agaagaatat aattttgagg gagttatacc aaatcttgaa cgactttttc
    10861 atcaaacaga aagtgaatac agaagagagg aaattttgaa atatataaga gaggttacct
    10921 gtccatcctg taaaggtggt agattgagac ctgaatccct tgctgttaaa atagcgggta
    10981 aatcaattat ggatatatgt aaaatgagtg ttgttaaagc aaaagaattc tttgaaaatt
    11041 taaaattgag tgaaaaagaa cagattattg caaaacagat tttaaaagag attctgtcac
    11101 gacttaattt tcttgaagaa gtaggacttg actatctgac acttgataga atgacacata
    11161 ccttatcggg tggtgaagca gaaaggataa gactggctac tcagattggt tcagggcttg
    11221 taggtgtttt atatattctt gatgaaccaa caatagggct tcatcagagg gataatttga
    11281 aacttataaa tacattgaaa catttaaggg atatcggcaa tactgttgtg gttattgagc
    11341 atgatgagga gacaattaaa aatgcagatt ttatagttga tcttggacct ggagcaggta
    11401 ttcatggagg agaagttgtt gcaaagggga cacttgaaga cattataaaa aatgaaaatt
    11461 caataactgg aaaatatcta agtggtaaat taaaaattga aataccgacc aaaagaagaa
    11521 aaccgggaga aaaaaaaata aaaattttaa aagcaagtca taataattta aaaaatattg
    11581 atgttgaaat tccacttggt ttatttgttt gtgttaccgg agtttctggt tctggaaaaa
    11641 gcagtttaat tgaagatgtt ttatataaag ggttgaagaa aattttatat gattcaaaag
    11701 aagaaccagg taagcatgat gcaattttag gaacagaata tataaataaa gtagttgtaa
    11761 ttgaccagtc gcctattggt agaacacccc gttcaaatcc tgcgacatat acaggtgcat
    11821 ttacctatat tagagaaata tttgcaaata cttatgaagc aaaaataaga ggttatacac
    11881 cagggagatt ca
//
LOCUS       DAOSAT010000057        11865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3630_length_11865_cov_5.585436, whole genome shotgun sequence.
ACCESSION   DAOSAT010000057 DAOSAT010000000
VERSION     DAOSAT010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11865
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3630_length_11865_cov_5.585436"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLF90_06060"
     CDS             complement(<1..343)
                     /locus_tag="PLF90_06060"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOK56876.1"
                     /translation="MKITGICGSPRGEKSNTKKLLLKFLEVCKEKGADTEIIDLSKYK
                     VGFCLACEICHKQPSCPLKDDRNLIVEKMLISDGIVFASPVYIDSVSAQLKGFFDRTS
                     HFIHCLRLNGKY"
     gene            448..1443
                     /locus_tag="PLF90_06065"
     CDS             448..1443
                     /locus_tag="PLF90_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK56877.1"
                     /translation="MKMKVGFVGCGGIAEAHLNALSNFPDVQLSAFCDIDEQRGKFIA
                     EKYKGKYYKNAKEMINNEELNCIFFCLPPFAHGAELYAIEKNIPFAVEKPVGLDLGLC
                     KEILEGVKEKKLLTCVLYMNRYRRGVKMVKDLSEKSQPVLILGGWIGGTPRQSEKGIW
                     KWWVQKDKSGGQFHEQVTHTVNLTRFFCGDAESVHAFAVKGKNKEVPPYYSIEDAVCV
                     NIKFKNGTIANLWASCSANAGGGGVSLDVYAVDFTAKFTGWEHTLRLFRKDQDIIEVR
                     GEPNIFEIEDRNFIDAVKTKNPSLIMSDYEDGFKTVEITLAANKSLEKGKVIEIK"
     gene            1456..2016
                     /locus_tag="PLF90_06070"
     CDS             1456..2016
                     /locus_tag="PLF90_06070"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HOK56878.1"
                     /translation="MRKNFLLIFLVLGLISCAVVNVYVTFPEEKVKKAAEDLLAPPSS
                     SKTKPESFLDNLFTRNLYAEEVVVTKDLKTDSPKISEAKEKMNTWREKLDEFKKSGYI
                     GETNDFRVVIRELPKNSEDAKEVRKIVDDENKQREIMIKELLRINNAAPDEEKKFRKI
                     FAEVAQKYSPQNTWIQLSDNSWTKKK"
     gene            2013..3071
                     /gene="vorB"
                     /locus_tag="PLF90_06075"
     CDS             2013..3071
                     /gene="vorB"
                     /locus_tag="PLF90_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOK56879.1"
                     /translation="MNKYLLTGCEAAAEAAIHAGCKFYAGYPITPQNRFPEYMSKRLP
                     EEGGIFIQAESELSAINMVFGASVCGFRALTSSSSPGISLKQEGISYLAGCELPAVII
                     NMNRGGPGLGDVTPSQSDYFQATRGGGHGDYRTIVYAPWTVQELYDLTYISFDIADKY
                     RNPVLILADGILAQIMESVKFKKRKNRLPIKKWALTGCYGRKPNSIKSLFLTPGVLEK
                     HNWKLYKKYKKIEEKEVKYEKYFCENSDSLVVSFGTCARICYESIKILRDKGKRIGLF
                     RPVTLWPFPYKELEKTIEKVKRIFVVEMNLGQMIDDVKIANRKNLPVYFYGRPGGGVP
                     TPQEISRFFTAHLKGGKE"
     gene            3075..3818
                     /locus_tag="PLF90_06080"
     CDS             3075..3818
                     /locus_tag="PLF90_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOK56880.1"
                     /translation="MEAVYKKPESLKEEITTYCPGCGHGIIHRLIAEIIDELNIREKT
                     IGVAPVGCAVLAYNFFNFDVTEAAHGRAPAVATAIKRLLPDRIIFTYQGDGDLAAIGT
                     AEIIHAAARGENFTVIFVNNAIYGMTKGQMAPTTLPGMKTTTTIFGREPNYHGYPLKV
                     CELLSQIDGVTYLERTAVDSIPGIIKTKLAIKKAFQYQIENKGFSLVEILSPCPVNWK
                     MSPSQSMKWIKEVMSKYFVPGVYKDKGGK"
     gene            3818..4477
                     /locus_tag="PLF90_06085"
     CDS             3818..4477
                     /locus_tag="PLF90_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HOK56881.1"
                     /translation="MFFLPFSLILLLIFFILLLPVFLFLIQIEIIRIALSKLGLSPAF
                     AFFILLLSLIGSGINIPIYSKEATIIPPSRDLFFLFQPFGLPQLQTEQVIAINVGGAI
                     IPILLCIYLLPKAPPLPTLFATIISTIVCFKLSRVIPGLGITIPALIPPLVSVSLAFL
                     FSPGNKIPVAYISGVLGVLIGADLLNLANLPPYPGVMSIGGAGVYDGIFLVGIISVLL
                     A"
     gene            4479..5186
                     /gene="radC"
                     /locus_tag="PLF90_06090"
     CDS             4479..5186
                     /gene="radC"
                     /locus_tag="PLF90_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOK56882.1"
                     /translation="MEKKKSLKYWKEEEKPREKLIKYGEHNLTDTELLAILLRSGTKG
                     ETAIDLARKIIDKFGSFRNMSHTSLNNFKEIKGLGMAKICQIKAAIEIGRRMVEKELI
                     KKEKPITSPEEVVRYIMPRMRDLKKEVFKVIHLDSKNRPVEIREIEEGTVNQANPIIR
                     EIFYKVIENFIPSIICVHNHPSGDPSPSKEDIEFTKRLKEAGEILGINVLDHIIIGDN
                     LYYSFASGKINTINSIF"
     gene            complement(5201..5677)
                     /locus_tag="PLF90_06095"
     CDS             complement(5201..5677)
                     /locus_tag="PLF90_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOK56883.1"
                     /translation="MNSQSIQSERPDWDTYFMEIAQLVSKRSTCIRRKVGAVLVKEKR
                     ILATGYNGAPTGLAHCKEVGCVREKLKIPSGERHELCRGLHAEQNAILQSAYHGVSIK
                     GSKLYITCHPCSVCAKMIINAGIEEVIIKEGYPDSMAENILKEGKIKVRYLNSEKQ"
     gene            complement(5652..6884)
                     /gene="glyA"
                     /locus_tag="PLF90_06100"
     CDS             complement(5652..6884)
                     /gene="glyA"
                     /locus_tag="PLF90_06100"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOK56884.1"
                     /translation="MLNKFDPEIYYALKKEEERQRNTINLIPSENIVSKNVLKLVGSV
                     FTNKYAEGYPGKRYYGGCEYVDMVEKIAIERACKLFKSDHANVQPHSGSQANMAVYFS
                     VLKPGDKILAMHISSGGHLTHGVKVNFSGMLYNTCFYSVDRETELIDYEKVREIAIKE
                     KPKIIVCGSSSYSRIIDFEKFSQIAKEVNAYLLSDIAHIAGLIVADLHPSPVGFSEFV
                     TSTTHKTLRGPRGGLILCNKEFKEKIDSIIIPGIQGGPLVHVIAGKAIAFKEAMTEKF
                     KKYQEQIVKNCKKLCEELKKRGYRIVSGGTDTHLFVIDLRDKGIIGFEAQITLEKVGI
                     ITNKNLIPFDPLSPSITSGIRIGTPCITSRGMKEKEMVLIAEWIDEGLKNRNNEKILN
                     EIRKKVRRFASEFPIYPE"
     gene            complement(6868..8955)
                     /gene="glyS"
                     /locus_tag="PLF90_06105"
     CDS             complement(6868..8955)
                     /gene="glyS"
                     /locus_tag="PLF90_06105"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOK56885.1"
                     /translation="MKKNLILEIGVEDLPSSIDEYLEEVFAPLLKKSLENERIDYNDL
                     KIFYTPRRIIIFLKDLEEKQKDKTVEISGPPLEICRDKDGNWNEIAYKFAKGNNVKLS
                     DLKIFEKKGKKVVGIVKLEKGESITKIFNRIINDVLKQLEIPRGMIWNENRFKFFRPI
                     RYIISLYGEKIVPVEIGGIKSKRFTYGHRTLSDKKIVPYDTKDFFDKLLKNFVIFDQE
                     LRKRMILEEIKKLIGDNFLFESKLVDKITPIVEYPVCGICNLPQNYKKLPKEVLTSII
                     LNVKGIPLFDKSGNLSPVFVVVCDGVFNNEIKGNYEKVVESKIEDACFFMEQDINKKP
                     FIEYLEDLKNIVYHPRFGTIYDRVERIRKICNFISESLNLDMKTRERIETIVSLFKND
                     LATLMVSEFPSLQGIIGRIYAEKNGYEFVISKSIEEHYLPKFQNDKKPEFIEGSIVSI
                     ADRIETICSFISEGAEIKGDQDPLGIKRLTTGMIEIIWEREIEISIEKLIEKTLEILD
                     IKSDEVKNIILEFILQRAENLLIAEGIKPGLRRAVLSVERDNLFEIKRKIEAIKNTFI
                     EGKGEEVLIPFIRVANMLKQAEERNIGYGQFDENLLIEEEEKRLYKFYIENSEKMYKL
                     YREKKYEEFLEEMTRWKEPIDKFFDKVFVMVENEKTRNNRLSLLKLIDDVFNKFANFS
                     YIPLKEVENVKQI"
     gene            complement(8976..10022)
                     /locus_tag="PLF90_06110"
     CDS             complement(8976..10022)
                     /locus_tag="PLF90_06110"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOK56886.1"
                     /translation="MYIFLNFLKFFFIISLLFVILFLIIQILDDLTDFLVHRKTFILK
                     NYLYTCPLLFVQISPIITTLSTMLILSEMLKNNEIKILFLSGINPRKVFSIFLFCGFF
                     ASVFTFVLKNYVSPPLMRKLSNRIIQSSIAFSSPKYFFYSEKLEDSYFINVEFSEYFE
                     DGRFITIKAKKAKNFSDLNWIFENGNLWEFDSEKNLIKKEKFSKKIVSILLTSEILSI
                     SSIDIETLSFFQLSSILNKMRKLGINPVSLTTYTYEKVSYPFLNFFLIFIIFSFLKKS
                     QKISNLYVFSFSFLFSFFSYFLYVFFFSLSKNAKIHPLLGSLGVPFVILAFIIFQNYG
                     LNKIKKSCIIFRKS"
     gene            complement(10034..11071)
                     /locus_tag="PLF90_06115"
     CDS             complement(10034..11071)
                     /locus_tag="PLF90_06115"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOK56887.1"
                     /translation="MKFKTKYIFKGFWINFFFSFFILLFIFMLKSFFQIFDLIVKGSF
                     SFLPLIKFFGFTVLASLEYIIPLTVLVASMSFFSTLYTDRELQIFAFSGISNLFLIKP
                     LIIFSIIFTLFLFYFNLFILPDIRFANINVIHQLKIRNPLSIIIEKEVIKDIPGTTIY
                     IEKVFRNFNLKNISITKRENNSTFFLKAEKGRVVYNPNENKLVFILENGNFLNYTEGS
                     INSVDFKNYEFYLELTEDFKRTEVKRQIPELNFIELKKIKSVEAMTELHERCVYALTP
                     LILLLLGSGIGMNLRQKNRILYIGIGGFISIIFFELLMVGEILVRKFEVPYFTYLPLF
                     IFALLTKKFWK"
     gene            11123..11710
                     /locus_tag="PLF90_06120"
     CDS             11123..11710
                     /locus_tag="PLF90_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOK56888.1"
                     /translation="MKKNCMGCEKDKENFYGFCNDCFSKIIFINETFKDRIHIGKYEG
                     PLKEAITRYKYEGRKYYAVNFASLIEDRVKKENIEFDIIIPVPLHWKKEFIRGFNQSA
                     LIGTYLSKKTKKRMYQNVMVKLKNTVSQTELSEKERKENVKDTFGIKNKDIIKDKIVL
                     LIDDVYTTGATTEECRKLLLKNGAKRVIITTIAKS"
BASE COUNT         4283 a         1766 c         1741 g         4075 t
ORIGIN      
        1 tatatttccc atttaatctg aggcagtgta taaaatgact tgttctatca aaaaaacctt
       61 taagttgggc agaaacactg tctatataaa caggagaagc aaaaacaatt ccatctgata
      121 taagcatttt ttcaactatt aaatttctat catcttttaa aggacaggat ggctgtttat
      181 ggcatatttc acaggcaaga cagaaaccta ctttatattt acttaaatca ataatttctg
      241 tatcagcacc tttttcctta catacttcaa gaaattttaa taataatttt ttcgtattgc
      301 ttttttctcc tcttggacta ccacaaatcc ctgttatctt catattatcc ctcttctttt
      361 tgttttatta tatcactctg cttgatttta tcaaagtaaa agtagtaaaa taaaagtaag
      421 ttgaaattta caaaaaagga ggaaaaaatg aaaatgaagg ttggttttgt tggatgtgga
      481 gggatagcag aggcacattt aaatgcttta tctaattttc cggatgtaca actttctgca
      541 ttctgtgata tagatgaaca aagaggtaaa tttattgcag aaaaatataa aggaaaatat
      601 tataaaaatg caaaagaaat gataaataat gaagaactta attgtatatt tttttgcctt
      661 cctccatttg ctcatggtgc agaactctat gcaatagaaa aaaatattcc atttgccgtt
      721 gaaaaacctg ttggacttga cttgggtttg tgtaaagaaa ttcttgaagg tgttaaggaa
      781 aaaaaattgc ttacctgtgt tttatatatg aacagatata gaaggggagt aaaaatggta
      841 aaggatttga gtgaaaaaag tcaacctgtt ttgatacttg gaggatggat aggagggacc
      901 ccaaggcaat ctgagaaagg aatatggaaa tggtgggttc agaaagataa gagtggaggg
      961 caatttcatg agcaggtaac ccatactgta aatttaacaa gatttttctg tggagatgct
     1021 gaaagtgttc atgcttttgc tgttaaagga aagaataaag aagtgcctcc ttattattca
     1081 atagaagatg cggtctgtgt aaacataaaa tttaaaaatg gaactatagc gaatctctgg
     1141 gcaagttgtt cagcaaatgc aggtggcgga ggtgtttctc ttgatgttta tgctgttgat
     1201 tttactgcaa aatttactgg ctgggaacat accctcagat tattcagaaa ggaccaggat
     1261 ataattgaag tgagaggaga accaaatatt tttgaaattg aagatagaaa ttttatagat
     1321 gcggttaaaa caaaaaaccc ttctttaatt atgtctgatt acgaagatgg atttaaaaca
     1381 gttgaaataa cacttgctgc aaataaatct ctggaaaaag gaaaggtaat tgaaataaaa
     1441 taaaggaggt ttgaaatgag gaaaaatttt ttattgattt ttttagttct tggattaatc
     1501 agttgtgcag tggttaatgt ttatgttaca tttccggaag aaaaggttaa aaaagcagca
     1561 gaagatttac ttgcaccacc atcctcttca aaaaccaaac cagaaagttt tcttgacaat
     1621 ttatttacaa ggaatcttta tgctgaagag gtagttgtaa caaaagattt aaaaactgac
     1681 tctcctaaaa taagtgaagc aaaagaaaaa atgaatacat ggagagaaaa acttgatgaa
     1741 ttcaagaaat caggatatat tggagagaca aatgatttta gagttgttat aagagaatta
     1801 ccaaaaaatt cagaagatgc aaaagaagtc agaaaaatag ttgatgatga aaataaacag
     1861 agagaaatta tgataaaaga acttttaaga ataaacaatg ctgcacctga tgaagaaaaa
     1921 aaattcagaa aaatttttgc tgaagtagca cagaaatatt ctccacaaaa tacatggatt
     1981 caactttctg ataattcctg gacaaagaaa aaatgaataa atatttactt acaggttgtg
     2041 aagcagcagc agaagcagca attcatgctg gatgtaaatt ttatgccggg tatcccataa
     2101 ctcctcagaa cagatttcct gaatatatga gtaaaagatt acctgaggaa ggaggtattt
     2161 ttattcaggc agagagcgaa ctttctgcta taaatatggt ttttggagca agtgtatgtg
     2221 gttttcgcgc attaacatca tcttcttctc cgggtatttc tttgaaacag gaaggaatat
     2281 catatcttgc tggttgtgaa ctacctgcag taattataaa tatgaatagg ggtggtcctg
     2341 gtcttggaga tgtaacgcct tctcaaagtg attattttca ggcaacaaga ggtggaggac
     2401 atggggatta cagaacaatt gtatatgctc cgtggacagt tcaggaacta tatgatttaa
     2461 cctatatttc atttgatatt gctgataaat atagaaatcc tgtcttaata cttgcagatg
     2521 ggatactcgc tcaaattatg gaatcggtaa aatttaaaaa aaggaaaaac agattaccta
     2581 taaaaaaatg ggctttaaca ggttgttatg gaagaaagcc aaattcaata aagtcattat
     2641 ttttaactcc tggtgtactt gaaaaacata actggaaact ttataaaaaa tataaaaaaa
     2701 ttgaggagaa agaagtaaag tatgaaaaat atttctgtga aaattcagat agtttagtag
     2761 taagttttgg gacatgtgca agaatatgtt atgaaagtat taaaatttta agggataaag
     2821 gtaaaagaat tggactattc agaccagtaa ctttatggcc atttccttat aaagaacttg
     2881 aaaaaacaat tgaaaaggta aaaagaattt ttgttgttga aatgaatctc ggacagatga
     2941 tagatgatgt taaaattgca aacagaaaaa atttaccagt ttatttttat ggaagacctg
     3001 gtggaggcgt ccccacacca caagaaatat caaggttttt cacagcccac cttaaaggtg
     3061 ggaaggaata agttatggaa gcagtatata aaaaaccaga aagtttaaaa gaagaaatta
     3121 caacatactg tcctggttgt ggacatggaa taatacatag attaattgca gaaataattg
     3181 atgaattaaa tattagagaa aaaacgattg gagttgctcc tgttggatgt gcagttttag
     3241 catataattt tttcaatttt gatgtaactg aagcagcaca tggaagagca ccagcagttg
     3301 caacagcaat aaaaagatta ttacctgata gaattatctt cacatatcag ggagatggag
     3361 accttgcagc aatagggaca gcagaaataa tacatgcagc agcaagagga gaaaatttta
     3421 cagttatttt tgttaataat gctatttatg gaatgacaaa aggacagatg gctccaacaa
     3481 ctttacctgg aatgaaaaca acaactacaa tttttgggag agaaccaaat tatcatggtt
     3541 atcctcttaa agtttgtgaa ttactttctc agattgatgg agttacttat cttgaaagaa
     3601 cagcagttga ttcaatccct ggtataataa aaacaaagtt agcaataaaa aaagcgtttc
     3661 aatatcagat tgaaaataaa ggtttttctc ttgtagaaat cctttcacca tgtcctgtta
     3721 actggaaaat gtcaccttcc cagtcaatga aatggataaa agaagttatg agtaaatatt
     3781 ttgtccctgg cgtttataaa gataaagggg gtaaataatg ttctttctgc ctttttcttt
     3841 aattttgctt ttgatatttt ttattcttct gttacctgtt tttctttttt taattcaaat
     3901 tgagataata aggattgctt tgagtaaact tggactatct cctgcttttg ctttttttat
     3961 tctccttctt tctcttattg gaagcgggat aaacatacct atttacagca aagaagcaac
     4021 aattattcca ccttcaagag atttattctt tttatttcaa cctttcggac ttcctcaatt
     4081 acagacagaa caggttatag ccattaatgt cggaggagca ataattccaa ttttactctg
     4141 catctattta ttacctaaag cacctcctct acctacttta tttgcaacta ttatctcaac
     4201 aattgtctgt tttaaattat caagagttat tccaggactt ggtattacaa ttcctgcttt
     4261 aattccccca cttgttagtg tttcacttgc atttttgttt tctcctggaa ataaaatacc
     4321 tgttgcctat atttctggag ttctcggtgt tttaataggt gctgatttac taaatttggc
     4381 aaatttacca ccctatcctg gagttatgag tattggtggt gcaggtgttt atgatggtat
     4441 ttttcttgtt ggtataatat cagttcttct tgcataaaat ggaaaagaaa aagtcattga
     4501 agtactggaa agaagaagaa aagccaagag aaaaactgat aaaatatggg gaacataatt
     4561 taacagatac agaacttctt gctattttat tgagaagtgg aacaaaaggc gaaacagcca
     4621 ttgaccttgc aagaaaaata attgataaat ttggttcttt caggaatatg agtcatacaa
     4681 gtttaaacaa ttttaaagaa atcaaaggac ttggtatggc aaagatatgt cagataaagg
     4741 cagcaattga aataggtaga agaatggtag agaaagaatt aataaaaaaa gaaaaaccga
     4801 taacatctcc tgaagaagta gtaagatata taatgccaag aatgagagat ttaaaaaaag
     4861 aggttttcaa ggttatacat cttgattcta aaaacagacc tgttgaaata agggaaatag
     4921 aagaaggaac ggtaaatcag gcaaatccaa taataagaga aattttttat aaggtgattg
     4981 aaaattttat cccatcaata atttgtgttc acaatcatcc atcaggagac ccaagcccga
     5041 gtaaagaaga tattgaattt acaaaaagat taaaagaagc aggtgaaata ctcggtataa
     5101 atgttttaga ccatataata attggagata atctttatta tagttttgca tcaggtaaaa
     5161 taaatactat caattcaatt ttctgattat caaggatttt ttactgtttt tctgaattaa
     5221 gataccttac ttttatcttt ccttccttca atatattttc agccatagaa tcaggataac
     5281 cttcttttat tatcacttct tcaattccag cattaataat cattttagca catacactgc
     5341 atggatgaca tgttatataa agtttacttc cctttattga aacaccgtga tatgctgatt
     5401 gtaaaatggc attctgttct gcatgaagtc ccctgcataa ttcatgtctt tctccagatg
     5461 gtattttaag tttctctctt acacaaccaa cttccttaca gtgagcaaga cctgttggtg
     5521 caccattata tcctgtcgca agaatcctct tttcttttac cagaaccgct cccactttcc
     5581 tccttataca tgttgacctt ttagagacaa gttgtgcaat ttccataaaa taagtatccc
     5641 agtcaggtct ttcactctgg atagattggg aattcacttg caaacctcct tacttttttt
     5701 cttatttcat ttaaaatttt ttcgttattt ctattcttta atccttcatc aatccactct
     5761 gcaattaaaa ccatttcttt ttctttcatt cctctgcttg ttatacaggg agtgcctatt
     5821 cttataccac ttgttataga tggagaaagg gggtcaaaag ggattaaatt tttatttgtt
     5881 attattccaa ctttttcaag agttatctgt gcttcaaaac ctattattcc tttatctctt
     5941 aaatctatta caaataaatg agtatctgtt ccaccactta caattctgta ccctcttttt
     6001 ttcaattcct cacacagttt tttacagttt tttacaatct gttcctgata ttttttaaac
     6061 ttttctgtca ttgcctcttt aaaagcaatt gctttaccag caatcacatg aacaagtgga
     6121 ccaccttgaa ttccaggaat tattatacta tctatttttt ctttaaactc tttgttacag
     6181 agaataagcc ctcctcttgg tcctctcaat gttttatgag ttgtagaagt aacaaattca
     6241 gaaaatccaa caggacttgg atgtaaatct gcaactataa gacctgcgat atgtgctata
     6301 tctgaaagaa gataggcatt aacttccttt gcaatctgag aaaatttttc aaaatcaatt
     6361 attcttgaat atgaactgct accgcataca ataatttttg gtttttcctt tattgctatt
     6421 tcccttactt tttcataatc aataagttct gtttctctgt ctactgaata aaagcaggta
     6481 ttgtaaagca ttcctgaaaa atttactttt actccatgag taagatgtcc tcctgaactt
     6541 atatgcattg caagaatctt atctcctggc tttaaaacag aaaaataaac agccatattc
     6601 gcctgacttc ctgaatgtgg ttgaacattc gcatggtctg atttaaaaag tttacatgct
     6661 ctttcaatag cgattttttc aaccatatcc acgtattcac aaccaccata atatcttttg
     6721 ccaggataac cttctgcata tttatttgta aagactgaac caacaagttt taaaacattt
     6781 ttacttacaa tattttcaga aggtattaaa tttattgtat ttctctgtct ctcctcttct
     6841 ttttttaatg cataataaat ttcagggtca aatttgttta acattttcca cctcttttaa
     6901 aggaatataa gaaaaatttg cgaatttatt aaacacatca tctatcaatt taagcagaga
     6961 caatctatta tttctggtct tttcattctc aaccataaca aaaactttat caaaaaactt
     7021 atctatgggt tctttccatc tggtcatctc ttcaagaaat tcttcatatt ttttctctct
     7081 atagagttta tacatctttt cagaattttc aatataaaat ttataaagac gtttttcttc
     7141 ttcttcaatt aaaagatttt catcaaactg tccatatcca atatttcttt cctctgcctg
     7201 ttttaacata tttgccactc taataaaagg aattaaaact tcttcacctt ttccttcaat
     7261 aaatgtattt ttaatcgctt ctattttcct ttttatctca aataaattat ctctctcaac
     7321 agaaagaact gctcttctta atcctggttt tattccttcc gctatgagta aattctctgc
     7381 tctctgtaaa ataaattcca aaattatatt tttgacttca tcacttttta tgtcaagtat
     7441 ttcaagagtt ttttcaataa gtttttctat tgaaatttca atttctcttt cccatataat
     7501 ttcaatcata cctgttgtta atcttttaat tccgagcggg tcctgatctc ctttaatttc
     7561 agcaccctca cttataaaac tacagatagt ttctattcta tcagcaatag agacaatact
     7621 tccttcaata aactctggtt ttttatcatt ctgaaatttt ggtaaataat gttcttctat
     7681 tgactttgaa atgacaaatt cataaccatt tttttctgca tatattcttc caataattcc
     7741 ctgtaaagat ggaaattcgc tcaccattaa tgttgcaagg tcatttttaa ataaagaaac
     7801 aattgtttct attctttctc ttgttttcat atccagattt aaactttcag atatgaaatt
     7861 acatattttt cttattcttt caactctgtc gtatattgtt ccaaatcttg gatgataaac
     7921 tatattttta aggtcttcca gatactcaat aaatggcttt ttattaatat cctgctccat
     7981 aaagaaacag gcatcttcta ttttactttc aacaactttc tcataatttc cttttatctc
     8041 attattaaaa accccatcac aaacaactac aaaaacagga gaaagattac cactcttatc
     8101 aaaaagaggt atacctttaa catttaaaat tattgatgtt aaaacctctt tgggtaattt
     8161 cttataattt tggggtaaat tgcaaattcc acaaacagga tattcaacta tgggtgtaat
     8221 cttatcaaca agtttagatt caaataaaaa gttatcacct atcaattttt taatctcctc
     8281 cagtatcatt ctttttcgta attcctggtc aaaaataaca aaattcttta aaagtttatc
     8341 aaaaaaatct tttgtatcat aaggaactat ttttttgtct gaaagagttc tgtggccata
     8401 agtaaatctt ttgcttttta taccccctat ttcaacagga acaatttttt ctccataaag
     8461 agaaattata tatcttattg gtctgaaaaa tttaaacctg ttttcattcc atatcatccc
     8521 tcttggaatt tcaagttgct taagcacatc atttattatt ctattaaaaa ttttagttat
     8581 actttctcct ttttctaatt taacaatacc tacaaccttt ttcccttttt tttcaaatat
     8641 tttcaaatca ctcaatttta cattgtttcc ttttgcaaat ttgtatgcaa tttcgttcca
     8701 attaccatcc ttatccctgc atatttccag tggaggtcct gaaatctcaa ctgttttatc
     8761 tttttgtttt tcttcaaggt cttttaaaaa aataataatt ctcctcggtg tataaaaaat
     8821 ctttaaatca ttataatcaa tcctttcatt ttcaagggat tttttaagta aaggtgcaaa
     8881 aacctcttcc agatattcat ctatacttga tggtaaatct tcaactccga tttctaaaat
     8941 aagatttttt ttcatttttt tcatcacttt caatgttaag atttcctaaa tataatacaa
     9001 gattttttta ttttattcaa accataattc tgaaaaataa taaatgccag aattacaaaa
     9061 ggaactccta aactacccaa aagaggatga attttggcat ttttggataa agaaaaaaag
     9121 aaaacataga ggaaatagga aaaaaaagaa aaaagaaaag aaaaagaaaa aacatataaa
     9181 tttgaaatct tctgactttt tttcaaaaag gaaaatatta taaatataag aaaaaaattc
     9241 agaaaaggat aagaaacttt ttcataagta tatgtagtta aagaaacagg atttataccg
     9301 agttttctca ttttatttag aatggaggaa agttgaaaaa atgaaagtgt ttcaatatca
     9361 atactggaaa tagaaagaat ttcagatgtt aacaaaattg aaacaatctt tttactgaat
     9421 ttttcttttt ttatcagatt tttttctgaa tcaaattccc atagatttcc attttcaaaa
     9481 atccagttta ggtcagagaa attttttgct ttctttgctt ttatagtgat aaatctccca
     9541 tcttcaaaat attcagaaaa ttcaacattt ataaaatatg aatcttctaa tttttcagaa
     9601 taaaaaaagt attttggaga agaaaaagca attgatgact gtataatacg attactcagt
     9661 tttctcataa gtggtggaga aacataattt ttaagaacaa aagtaaaaac agatgcaaaa
     9721 aaaccacaga aaagaaatat tgaaaatact ttccggggat taatacctga aagaaaaaga
     9781 atttttatct cattattttt caacatctct gaaaggatta acattgttga aagggttgtt
     9841 ataattggag atatctgtac aaataaaaga gggcaggtat ataaataatt cttcaaaata
     9901 aatgtttttc tgtgaaccag gaaatctgtt aaatcatcaa gaatctgaat tataagaaaa
     9961 agtatgacaa ataaaagaga tattatgaaa aagaatttca gaaaatttaa aaatatatac
    10021 aaaaactttt ttctcatttc cagaattttt ttgttaataa agcaaagata aaaagtggta
    10081 aataagtaaa atatggcact tcaaattttc ttacaagtat ttcacctacc attaaaagtt
    10141 caaaaaatat tatacttata aatcctccaa tccctatata caaaatcctg ttcttctgtc
    10201 ttaaattcat tcctatacca gaaccgagta aaagtaaaat taaaggcgta agagcatata
    10261 cacacctttc atgaagttca gtcattgctt caacactttt aatcttttta agttctataa
    10321 aatttagttc aggaatttgt cgtttaactt ctgtcctctt aaaatcttct gttaactcaa
    10381 gataaaactc ataattttta aaatcaactg aatttataga accttcagta taatttagaa
    10441 aattcccatt ttcaaggatg aatacaagtt tattctcatt cggattataa acaaccctac
    10501 ctttctctgc ctttaaaaag aatgttgaat tattctctct ttttgtaatt gaaatatttt
    10561 ttaaattgaa atttctaaaa accttttcaa tatatattgt tgttcctgga atatctttta
    10621 ttacctcctt ttcaattatt attgaaagag gatttcttat tttcaattga tgtataacat
    10681 ttatatttgc aaatcttata tcgggtaaaa taaaaagatt gaaataaaaa agaaaaagtg
    10741 taaaaataat agaaaaaata attagaggtt taatcagaaa taaatttgaa atacctgaaa
    10801 atgcgaaaat ttgtagttct ctatcagtat aaagagttga aaaaaaagac attgaagcaa
    10861 caagcactgt tagaggtatt atatattcaa gtgatgccaa aactgtaaaa ccaaaaaatt
    10921 taataagtgg taaaaatgag aaactacctt taacaattaa atcaaaaatc tgaaaaaaac
    10981 ttttaagcat aaaaataaaa agtaatataa aaaaggagaa gaaaaaatta atccaaaaac
    11041 ccttaaaaat atattttgtc ttaaatttca taaaatctat tgtaaaataa ataactttat
    11101 atgtctaatt ggcaggaatt tattgaagaa aaactgtatg ggttgtgaaa aagataaaga
    11161 aaatttttat ggtttttgca atgactgttt ttctaaaatt atttttatta atgaaacatt
    11221 taaagacaga atacatatag gaaaatatga aggaccttta aaagaagcaa ttaccagata
    11281 taaatatgaa ggaaggaaat attatgcagt aaattttgcc tcactcattg aagatagagt
    11341 aaaaaaggaa aatattgaat ttgacataat tatacctgtt ccccttcact ggaaaaaaga
    11401 atttataaga gggtttaatc agagtgcttt aataggaacc tatctttcaa aaaaaactaa
    11461 aaaaaggatg taccagaatg ttatggtaaa actaaaaaac acagtctccc aaacagaatt
    11521 aagtgaaaag gaaagaaaag aaaatgtaaa ggatactttc ggaataaaga ataaggatat
    11581 tataaaggat aaaatagttc ttcttataga tgatgtttat actacaggtg caacaacaga
    11641 agagtgcaga aaacttttac ttaaaaatgg agcaaaaaga gttataatta ccacaattgc
    11701 aaaaagttaa agattcattc aacagtcaca gatttagcaa gattcctcgg tttatcaaca
    11761 tcaagacctt tttttctggc aacataataa gaaagcaact gtaagggtag aattgttaaa
    11821 aatactttta aatactcatt ttttatatgt ggaataagta taatc
//
LOCUS       DAOSAT010000058        11430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3791_length_11430_cov_4.571077, whole genome shotgun sequence.
ACCESSION   DAOSAT010000058 DAOSAT010000000
VERSION     DAOSAT010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11430
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3791_length_11430_cov_4.571077"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..72
                     /locus_tag="PLF90_06125"
     tRNA            1..72
                     /locus_tag="PLF90_06125"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33..35,aa:Ala,seq:cgc)
     gene            81..848
                     /gene="surE"
                     /locus_tag="PLF90_06130"
     CDS             81..848
                     /gene="surE"
                     /locus_tag="PLF90_06130"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOK56889.1"
                     /translation="MRILLTNDDGIFSDGLKILYKYLKEIAEIFIVVPETEKSATSHS
                     INLLNPVRVKEVKINNFLCFVVSGTPVDCVKIGVRKLIKEKIDMVISGINPGPNLGMD
                     ILYSGTVSAACEGAILGIPSIAVSIADYKNFKFDECSKVVLKLMDKIKNLKFPKDTIL
                     NINIPNRKINEIKGIKIAYQSKSRFNEEYEKRIDPRGNIYFWLKGNFKEVKGEKYSDV
                     DAVKNGYVSITPIQLNLTNFNFLKILKAGKFEKFKFK"
     gene            880..1014
                     /gene="rpmH"
                     /locus_tag="PLF90_06135"
     CDS             880..1014
                     /gene="rpmH"
                     /locus_tag="PLF90_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOK56890.1"
                     /translation="MSITYRPHKLRRKRKIGFLARMSTKSGRKIINNRRRKGRKRLAG
                     "
     gene            1011..1304
                     /locus_tag="PLF90_06140"
     CDS             1011..1304
                     /locus_tag="PLF90_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOK56891.1"
                     /translation="MIKKRIREILKKGKKIETEYFKLYLNKRNDLKIGFLISSKIGKV
                     VKRNKAKRIIREIVRNNFKKGDFILVLKKEIIEKRKIEEVFEKIKNEIYNSIN"
     gene            1279..1494
                     /gene="yidD"
                     /locus_tag="PLF90_06145"
     CDS             1279..1494
                     /gene="yidD"
                     /locus_tag="PLF90_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOK56892.1"
                     /translation="MRFIIQLIRFYQILFSPVFGRVCRFYPSCSEFMIQAIEKKGFKG
                     ILLGIRRILRCHPFNKGGYDPVEKWIK"
     gene            1482..3023
                     /locus_tag="PLF90_06150"
     CDS             1482..3023
                     /locus_tag="PLF90_06150"
                     /inference="COORDINATES: protein motif:HMM:NF026199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HOK56893.1"
                     /translation="MDKINEEIRLLIAFTLSILIIFIFSRFQTTKNSPSQYKKIQQEQ
                     QILTPETKTEIIQIPEGETIEFENKDYYLTLDKKGGYIKEIGLKKFLRKNEKFFPLSD
                     GNLFFYTPGVHSEFEYNLKISNLNNEVFLTKETPDFYYQKKFRVPENNYIFQYEEILK
                     NKTDKDTELKDYRIEILKINLQNIKAQRSYIPPKIIVGMNKFSVFIDPYKIKEKKIYK
                     NCNWVGYSSRYSLVFIYIPEKSNITIEPDSNFINLVLLYENLNFHKGEEKTYQFKFYA
                     GPSDYFIARNYIKEDVFGKGFFTVIGRFLFFILNGIHKVIPNWGLAIIILTFIIKILF
                     FPLTRKSLYSMKQLQKLRPYLQDIQKKYKDNPYQLQKELMNIYKEYNINPFAGCLPTI
                     IQIPIFIGFFLALRNSIFLRGAPFILWIKDLSMPDTVFKINGFPVNILPILMTITSYF
                     QQKLTPQTEPSQKSLTILMPLMFLFILYNFPSGLLLYWVTMNIASLIEQYFVSTNSFR
                     IKLKK"
     gene            complement(3024..6083)
                     /locus_tag="PLF90_06155"
     CDS             complement(3024..6083)
                     /locus_tag="PLF90_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56894.1"
                     /translation="MKKLFIFLFFPFKFIFSDILFDFKKFEKLEEGNLRYKLNLEKDG
                     IYFLKFEGAFPDNIQNEGIRYLLKINGKEILHKQEWNEGYIPTIIPVEKKRKSFIFLY
                     TVPLNLKKGENEIIFASPENSKLSTLKLIEGIEGEIKKEVKSTGTNFIFFKSEEVNIS
                     FNLVSDRDRKIEYEIISYFSILSDDKGEGIWSPGNRFIAKDMVYRSSGKTEIKKNEKK
                     EFSLKLPSSKYGTFSLFFVLKENDRVLPLHIMNYSIVYERDTENFNYDGLLMASCGKE
                     EFLKGLKKIGVDWVRYEVGWNGFEPKKGEFKWYMDKFMEECRKNKIYVMTLTEGAPEW
                     AKPKGDFFDIPYKNFKIKLDWSPGREFYDDWKNAWYEYGKRYKDVNKAFNMWNEPWEG
                     GGISGWKSTGEHYRNLFKKVKEARDMISSDIKMVGADSSHNTNWKIFAAGMDKDVDVL
                     STHYELPVQACYSPALSNYYGKEVWDTETWMTWMGDSATVRNLLAEIALGWKKVSLYV
                     TNLIFDKNGFPNTSVAWSSAFARFLNNKEFKGFAHPERPPFVLLFNGKNENVAVATTS
                     LCGVYQWKDAVFWGQFSEEKPIMIIKEISDLKVYDIYGNELKIEKRKGKILIPVDKNP
                     KYLVTKTEFYNFKNILSSAEYDKLRPCEIVVHQIGKDNELNVDLKNVHRKKVKGILFI
                     KSSGIEYEEDRFNFTLEPLEKKILKIRIKKYEKDINRFPSEIIVKTDIGEARLNEDLV
                     ICVVKKGKIQVDGDIDEWKSIGAIPIILIKGEKGEDLTLKAWYPWENFVNEMPDFAAE
                     VSFAYDDEFLYGMARVKDKTKDILPSLLSGKNLHKFQNPPGDYVYAEIGPIPAASGDM
                     IKISLGNIERKNWIKEYEVFSPGSPLYRFGHYLSALYQYLIYPTNDGNAEIMRVRTPE
                     FYYLHPLPIDYKFLSEKCKIEKSKVIVKSDENGYIYEFAIPWEELKDIKFDKNKRIKL
                     SFEIQDKGMGNVIEFSKNKSICSVNILDFEPGWGAKWTAETEFEFSD"
     gene            complement(6080..8791)
                     /locus_tag="PLF90_06160"
     CDS             complement(6080..8791)
                     /locus_tag="PLF90_06160"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOK56895.1"
                     /translation="MKILKNFVLYMILFFSIFSFSEEFQVSPYVSPEFHKNIPWPKQS
                     FYLQPWRSYMETVRGKEFLEGIGIAYNFPWAVGDNETALKILSNSGFKNIRFEIGWSN
                     VEWDEKKLKNEEKLIDVFKICKKYGIKPLILLNAHHGYPCPMKYMNGEVLEDVSIGSK
                     EIKIKVQGEIKEGYTALTNLKGKDPSHAAYDIFITKYQEETASLSRPLPNDFKKGDKI
                     QLRIYKYLPAHPVGTPEFEEMADGWMKYVKLVCDMLKKAGLEDKDFELEIWNELSFGS
                     MFIADHGINFYYDPPKVKFSGDFLKKGGHAWEIGKRTVDYVKENYPGTRVIWGFSNTT
                     FYHTSILQLPPKIDGQSYHPYGTGKRVVSKDYPPKDRYSWYIEGYIPDNLTWCMPEGW
                     AHLGVQTERLTHHLFPLNRKARPEGVERFYHYLTEHGFNGYEAGISDEEEALKYKAKT
                     IIRALLFWLNKGISKVYIFCTYDKDNRGMGILLSNINPKEYSKYPEDQLYSPAMKALK
                     NVFDKFKDSEDLKEVRQIDIEVVSLGKQYKVFENVKYVGEKKENIPNPPELYYRDMFT
                     FLPFQINKNKFVFAVYVMSYDITNPLPSMNFRLIIKNIYGENAKITYYDPIENKEIPF
                     KLLDKGKDNVKLEIEAVEYPRLIIIEEKKVPVSIKEIKILDTGTKTATIQVKTNIPSK
                     ISINFYPSISSQFENVWTSKTYSNLTNVKLDNLFPGLKYEFKVEAEDKNGVKIISPSY
                     VWEKNYFFETEKDPKSLPLGLKVEYYTSSKPGEFTNLKKTEITPFVYIDEKLLKEKVG
                     QTEMVAVRYTGFIYIDKDDKYTFYTVSDDGIRISVDDKQIINNWTHHAVKEDIGEIEL
                     KKGWHKILIEYFQGAGGALLNFYYSRSDLKKSLIQPVYFKSEQGGDK"
     gene            complement(8809..9555)
                     /locus_tag="PLF90_06165"
     CDS             complement(8809..9555)
                     /locus_tag="PLF90_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOK56896.1"
                     /translation="MAKKSIFSKPHGFTLIELLVVVAIIAILAAMLLPALSKAREKAR
                     QAVCMNNLKQIGQALHMYINDYDSWFPSYFCGGPVIGAFYYDFLLVNYTNPSAKVKDW
                     NDYFTRAYSNNNKDGVGRIYQCPSHRNITTGDWYNRRRSYCIIEPSSFRGFKKTLPSG
                     TVIASFKYEQIRSPSSKFFLFERCDTNVLATNWSIFTAPFGVNDCFTNGLSTPYLKFG
                     NVLFCDGHVESYSQEMVKKLADYDFKVVFP"
     gene            complement(9602..10696)
                     /locus_tag="PLF90_06170"
     CDS             complement(9602..10696)
                     /locus_tag="PLF90_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK56897.1"
                     /translation="MALYKKIRDDIKEKIEKGEYKVGEKIPSISELSKIYNVSDIVVK
                     QALKELIKEGLLIGVQGRGVFVKEKENEKIIGVIFTDIVKNPFFAEIYTGIEKVFSSN
                     GYHIIVGVSYNNVEKEKKILKEFIERKVSGIIMTPTEVNRTSSDNNIFYELKERRIPF
                     IFVDRTIEDIETDYVTSDNFKGGYIATKYLISLGHRRIGIILGINANTVRERLQGYKK
                     ALEEENIEFDPMLVKRSYTELQYDESGYTNTKELLHLKNPPTAIFATNDPIAFGVYKT
                     CIEMDIKIPDDLSVIGFDKAHFSSILNPVLTTIEQKTEEIGEKASEILIKRINGDKSP
                     IQKIILDVNIVEGFSCKKLFKKINVKGGAL"
     gene            10931..>11430
                     /locus_tag="PLF90_06175"
     CDS             10931..>11430
                     /locus_tag="PLF90_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56898.1"
                     /translation="MNKINSMISFKSIVIGLIFCILIGIGEPLNVLYIHGSALAADYS
                     TGAAIFLFFLLVGILNQFNKKFIKKLYLTPPELITVYIMMIVACAIPSWGFTMNLIGL
                     LGGIFYYATPVNEWDKFIHPYLPKHLFPKDYNAIWYLYEGLPKNMKIPWEEWILPLFN
                     WFSFIIA"
BASE COUNT         3878 a         1886 c         1382 g         4284 t
ORIGIN      
        1 ggggcagtag ctcaacggga gagcgtccgc ttcgcatgcg gaaggttgcc ggttcaagtc
       61 cggtctgctc caatattaaa atgagaatac ttttaacaaa tgatgatggt atattttcgg
      121 atggattgaa aatactttat aagtatttaa aggaaattgc agaaattttt attgttgttc
      181 ctgaaacaga aaaaagtgca acaagtcatt ctattaattt acttaatcct gtaagggtaa
      241 aggaagttaa aataaataat tttttatgtt ttgttgtaag tggaactcct gttgactgtg
      301 taaaaatagg tgtaaggaaa ttaataaaag aaaaaattga tatggtaatt tcaggaataa
      361 atccaggacc aaatcttgga atggatatat tatattcagg gactgtttct gctgcctgtg
      421 aaggagcaat ccttggaatt ccttcaattg ctgtttcaat agcagattat aaaaatttta
      481 aatttgatga gtgttcaaaa gttgttttaa agttaatgga taaaataaaa aatctaaaat
      541 tcccgaaaga tacaattttg aatataaata tacccaacag gaaaataaat gaaattaaag
      601 gaataaaaat agcttatcaa agtaagagca ggtttaatga agaatatgaa aaaaggattg
      661 acccacgagg caatatttac ttctggctaa aaggtaattt taaagaagta aaaggggaaa
      721 aatacagtga tgttgatgca gtaaaaaatg gatatgtttc tatcactcca atacaactta
      781 atctcacaaa ttttaatttt ttaaaaattt taaaagcagg aaaatttgaa aaatttaaat
      841 ttaagtagta aaataatact tttataacgg aggtctgaaa tgtcaataac atatagacca
      901 cataaattga gaagaaaaag gaaaattgga tttcttgcaa ggatgtcaac gaaaagtgga
      961 aggaagatta tcaacaatag aagaagaaaa gggagaaagc gacttgctgg atgattaaaa
     1021 agagaataag ggagatactt aaaaaaggaa agaaaataga aacagagtat tttaaattat
     1081 atcttaacaa aagaaatgat ttgaaaattg gtttcttgat aagttcaaaa ataggcaagg
     1141 ttgtgaaaag gaataaggca aaaagaataa taagagaaat agtaagaaat aacttcaaaa
     1201 aaggtgattt tatattagtt ttgaaaaaag aaattattga aaaaagaaaa attgaagaag
     1261 tttttgagaa aataaaaaat gagatttata attcaattaa ttaggtttta tcaaatcctg
     1321 ttttcacctg tttttggaag ggtttgtaga ttttatccaa gttgttctga atttatgata
     1381 caggcaatag aaaaaaaagg ttttaaaggt attttattag gtataagaag gatactgaga
     1441 tgtcatccat ttaataaagg tggatatgac ccggtggaaa aatggataaa ataaatgaag
     1501 aaataagatt gttaattgca tttacacttt caatattaat tatatttatt ttcagcagat
     1561 ttcaaacaac aaaaaattct ccctcacaat acaaaaaaat tcaacaggag caacagatat
     1621 taactcctga aacaaaaaca gaaattatac agatacctga aggtgaaaca attgaatttg
     1681 aaaataaaga ttattactta acacttgata aaaaaggtgg atatataaaa gaaattggtc
     1741 ttaagaaatt tttgagaaaa aacgaaaaat tttttcccct gagtgatgga aatttatttt
     1801 tttatactcc tggagttcat tcagaatttg aatacaattt aaaaatttct aatcttaata
     1861 atgaagtttt tttaacgaaa gaaactcctg atttttatta ccagaaaaaa tttagagtac
     1921 ctgaaaataa ttatattttc cagtacgaag aaattctcaa aaacaaaaca gataaagata
     1981 cagaacttaa ggattataga attgaaatct taaaaattaa cttacaaaac ataaaagcac
     2041 aaagaagtta tattccacca aaaataattg ttggaatgaa taaattttca gtttttattg
     2101 acccatataa aattaaagaa aaaaagattt ataaaaattg taactgggtt ggatattctt
     2161 caagatattc tcttgttttc atttatattc cagaaaaatc caatataaca attgaacctg
     2221 attcaaattt tataaattta gtccttttat atgaaaattt gaattttcac aaaggagaag
     2281 aaaaaacata ccagtttaaa ttttatgcag gaccatctga ttactttatt gcaagaaatt
     2341 acataaaaga ggatgttttt ggaaaaggtt tttttacagt aattggaagg tttttgtttt
     2401 ttattttaaa tggtattcat aaagttattc caaattgggg attagcaata atcattttaa
     2461 cttttataat aaagatttta tttttcccat tgacaaggaa aagtttatat tcaatgaaac
     2521 aactccaaaa attaagaccg tatttacagg atatacaaaa aaaatataag gacaatccct
     2581 atcaattaca gaaagaactt atgaatattt ataaagaata caatataaat ccatttgcag
     2641 gctgtcttcc aactataatt caaatcccta tatttatagg tttttttctt gctttaagaa
     2701 attctatatt tttaagagga gcacctttca tattatggat aaaagacctc tctatgcctg
     2761 atactgtttt taagataaat ggttttccag tgaatatctt accaatttta atgacaataa
     2821 cttcttattt ccagcaaaaa ctaactcccc aaacagaacc aagtcagaaa tcattaacaa
     2881 tacttatgcc attgatgttt cttttcatcc tttataattt cccatcaggt cttttactct
     2941 actgggttac aatgaatata gcaagtttaa ttgaacaata ctttgtttcc acaaattctt
     3001 tcagaataaa acttaaaaaa taattaatca gaaaattcaa attctgtctc tgcagtccat
     3061 tttgctcccc aaccaggttc aaagtcaagt atattgacag aacaaattga tttatttttt
     3121 gaaaattcta taacatttcc cattccttta tcctgaattt caaaactcaa ttttattctt
     3181 ttgtttttgt caaatttaat atccttcaat tcttcccatg gaattgcaaa ttcatatatg
     3241 tatccatttt catcgctttt tacaataact tttgattttt caattttaca tttttctgat
     3301 aaaaatttat agtcaattgg aagtggatgc agataataaa attcaggtgt tcttactctc
     3361 attatttctg cattaccatc atttgttgga taaattaaat actgatataa agcagaaaga
     3421 taatgtccaa atctatataa tggagaacct ggtgaaaaaa cttcatattc tttaatccag
     3481 ttttttcttt caatgtttcc aagtgagatt tttatcatat caccacttgc tgcaggaatt
     3541 ggtccaattt ctgcataaac ataatcaccc ggaggatttt gaaacttatg tagatttttg
     3601 cctgaaagta aagaaggaag tatatccttt gttttatctt ttactcttgc cattccatat
     3661 aaaaattcat catcataagc aaaagaaact tctgctgcaa aatcaggcat ctcattaaca
     3721 aaattttccc atggatacca tgccttcaat gttaagtcct ctcctttttc ccctttaatt
     3781 aaaattatag gtattgctcc aattgatttc cattcatcta tgtctccatc aacttgaatt
     3841 ttaccctttt taactacaca aattacaagg tcttcattta atcttgcttc acctatatct
     3901 gttttgacaa ttatctctga tggaaatcta tttatgtctt tttcatattt tttaatcctt
     3961 attttcaaaa tctttttttc aagtggttca agtgtaaaat taaatctgtc ttcttcatat
     4021 tcaataccag aactttttat aaacaatatt ccttttactt ttttcctatg aacattcttt
     4081 aaatccacat tcagttcatt atccttacct atttgatgaa caacaatttc acatggtctt
     4141 aacttatcat attcagcact acttaaaata tttttgaaat tataaaattc tgtctttgtt
     4201 acaagatatt ttggattttt gtcaacagga attaaaattt tgccttttct cttttcaatt
     4261 ttcaattcat taccataaat atcatatacc ttcaaatcag aaatttcttt tataatcata
     4321 attggttttt cttcagaaaa ttgtccccag aatactgcat ctttccactg ataaacacca
     4381 cataatgaag ttgttgcaac agcaacattt tcattctttc cattaaaaag taaaacaaaa
     4441 gggggccttt caggatgtgc aaatccctta aattctttat tattcaaaaa tctcgcaaaa
     4501 gcacttgacc aggcaacaga tgtgtttggg aaaccatttt tatcaaaaat caaatttgtt
     4561 acataaagag aaactttttt ccagccaaga gcaatttcag caagaagatt tctaactgtt
     4621 gcactgtctc ccatccatgt catccatgtt tctgtatccc agacctcttt tccataataa
     4681 tttgaaagtg caggcgaata acatgcctga acaggtaatt cataatgggt tgataaaaca
     4741 tctacatctt tatccattcc tgctgcaaat atcttccagt ttgtattatg agaagaatca
     4801 gcacctacca tttttatatc agaagaaatc atatcccttg cttctttaac ttttttaaat
     4861 aaatttctat aatgttctcc tgttgacttc catccactta ttccaccgcc ttcccatggt
     4921 tcattccaca tattaaatgc tttatttaca tctttatatc tctttccata ttcataccat
     4981 gcatttttcc aatcatcata aaattctctt ccaggagacc aatcaagttt tatcttgaaa
     5041 tttttataag gtatatcaaa aaaatcacct tttggttttg cccattcagg tgcgccttca
     5101 gttaatgtca taacatagat tttgtttttt ctacattctt ccataaattt atccatatac
     5161 catttaaatt ctcccttttt aggttcaaat ccattccaac caacttcata tctaacccag
     5221 tcaacaccta ttttcttaag tccttttaaa aactcctctt ttccacagga tgccattaat
     5281 aaaccatcgt aattaaagtt ctctgtatcc ctttcgtaaa caattgaata attcataata
     5341 tgtaggggta aaactctgtc attctctttt aaaacaaaaa ataaagaaaa tgttccatat
     5401 ttacttgaag gcaattttaa agaaaactct tttttctcat tctttttaat ttctgtcttc
     5461 ccactgcttc tataaaccat atcctttgct ataaacctat ttccaggaga ccaaataccc
     5521 tctcctttat catcagaaag tatggaaaaa taagaaataa tctcatattc aatttttcta
     5581 tccctatcgg atactaaatt aaaagaaata ttgacttctt cacttttgaa aaagatgaaa
     5641 tttgtacctg tcgattttac ttctttttta atttctcctt caattccttc aatcaacttt
     5701 aaagtagata attttgaatt ttcgggtgaa gcaaatatta tttcattttc tccttttttc
     5761 aaattaagtg gaacagtgta aagaaaaata aatgattttc tcttcttttc aactggtatt
     5821 atagttggta tatatccctc attccattcc tgtttatgta aaatttcttt tccatttatt
     5881 ttaagtaaat atctaatacc ttcattctga atattatcag gaaatgctcc ttcaaacttc
     5941 aaaaaatata taccatcctt ttcaagatta agtttatacc ttaaattccc ttcttccaac
     6001 ttttcaaatt ttttaaaatc aaacaatata tcggagaata taaacttaaa gggaaaaaat
     6061 aaaaaaataa ataacttttt catttatctc ctccttgttc tgatttaaaa taaactggtt
     6121 gaattaaact cttttttaaa tcactcctgc tataataaaa attaagcaat gctccaccag
     6181 ccccttggaa atattcaatt aaaatcttat gccaaccctt ttttaattca atctctccta
     6241 tatcttcttt tactgcatga tgtgtccagt tatttataat ttgtttatca tctacggaaa
     6301 tccttattcc atcatctgaa actgtgtaaa aagtatattt atcatcctta tcaatgtaaa
     6361 taaatccagt atatcttact gcaaccattt ctgtttgccc tactttttcc ttcaataatt
     6421 tctcatcaat atagacaaat ggagtaattt ctgttttttt aagatttgta aattcacctg
     6481 gcttacttga agtataatac tcaactttta aacccaaagg caaacttttt gggtcttttt
     6541 ctgtctcaaa aaaataattt ttctcccata cataagaagg tgatattatt tttactccat
     6601 ttttatcctc tgcttcaacc ttaaattcat attttaaacc aggaaaaaga ttatcaagtt
     6661 ttacatttgt taaattagag tatgtctttg aagtccatac attttcaaat tgggatgaga
     6721 ttgatggata aaaattaatt gaaatctttg aaggtatatt tgtttttact tgaatagtcg
     6781 ctgtttttgt cccagtatca agaattttaa tttctttaat tgaaacaggg actttttttt
     6841 cttcaattat tatcaatctt ggatattcaa ctgcctctat ttcaagtttc acattgtctt
     6901 ttcctttatc taaaagttta aatggaattt ctttattttc aattgggtca taatatgtaa
     6961 tttttgcatt ttcaccataa atatttttta ttattaatct gaaattcata gaaggaagtg
     7021 gatttgtaat atcataactc ataacataga cagcaaatac aaacttattt ttattaatct
     7081 gaaatggtaa aaatgtaaac atatctctgt aatagagttc aggtggattt gggatatttt
     7141 ctttcttttc tccaacatat ttcacatttt caaatacctt atattgtttc ccaagagaaa
     7201 ccacttctat atcaatttgc ctcacttctt tcaagtcctc tgagtcctta aatttatcaa
     7261 aaacattttt taatgctttc atcgctggtg aataaagttg gtcttctgga tattttgagt
     7321 attcctttgg atttatattt gataacaaaa tccccattcc tctattatct ttatcatatg
     7381 tgcagaaaat ataaaccttt gaaattcctt tatttaacca aaaaagtaat gctcttataa
     7441 ttgtctttgc cttatatttt aatgcttctt cttcatctga aatacctgcc tcataaccgt
     7501 taaatccgtg ttctgttaaa taatggtaaa atctttcaac tccttcaggc cttgcttttc
     7561 tattaagtgg aaataaatgg tgagttaatc tttctgtctg gactccaaga tgagcccaac
     7621 cctctggcat acaccaagtt aagttatcag gaatatatcc ttcaatatac catgaatacc
     7681 tatcttttgg aggataatct tttgagacaa ctcttttacc tgttccatat ggatgatatg
     7741 actgaccatc tatttttggc ggaagttgta aaatagatgt gtgataaaat gttgtatttg
     7801 aaaatcccca tataaccctt gttcctggat aattttcttt tacataatca actgttcttt
     7861 tgcctatttc ccatgcatgt cctccctttt tcaaaaaatc accagagaat ttaacctttg
     7921 gagggtcata ataaaaattt attccatggt ctgctataaa cattgaacca aaagataatt
     7981 cattccatat ttcaagttca aaatccttat cctcaaggcc tgcctttttt aacatatcac
     8041 aaacaagttt tacatacttc atccatccat ctgccatttc ctcaaattca ggtgttccta
     8101 ctggatgtgc aggtaaatat ttgtatatcc ttaactgaat tttatcacct tttttaaaat
     8161 catttggtaa tggtctactt aaacttgctg tctcttcttg gtattttgtt atgaatatgt
     8221 catatgcagc atgtgatggg tctttccctt ttaaatttgt tagtgcagta tatccttctt
     8281 ttatctctcc ctgtaccttt atctttattt ctttgcttcc tatacttaca tcctcaagaa
     8341 cctctccatt catatatttc ataggacagg gataaccatg atgtgcattc aataaaatta
     8401 atggctttat accatatttc ttgcagattt taaaaacatc tataagtttt tcctcatttt
     8461 taagtttttt ctcatcccat tcaacattac tccatccaat ctcaaatctt atatttttaa
     8521 atccactatt tgaaagaatt tttaaagcag tttcattatc ccctactgcc caagggaaat
     8581 tataagcaat accaatacct tcaagaaatt cctttcctct aactgtctcc atataacttc
     8641 tccatggttg aagataaaat gattgttttg gccaaggtat atttttgtga aattcaggac
     8701 ttacataagg tgaaacctgg aattcctcag aaaaagaaaa aatactaaaa aaaagaatca
     8761 tatataaaac aaaatttttt aatatcttca tattccctcc tcttttattc aaggaaatac
     8821 tactttgaaa tcataatcag caagtttttt aaccatttcc tgagaatatg attcaacatg
     8881 tccatcacag aataaaacat ttccaaattt taaataaggt gtacttaatc catttgtaaa
     8941 acaatcattc acaccaaatg gtgcagtaaa aatagaccag ttagttgcaa gaacatttgt
     9001 atcacatctt tcaaaaagaa agaattttga acttggactt cttatttgtt catatttaaa
     9061 acttgcaatt actgttcctg aaggaagagt ttttttaaaa cccctaaaag aagatggttc
     9121 aataatacaa taactccttc ttctattata ccagtcccca gttgttatat ttctgtgtga
     9181 agggcattga taaatccttc ccactccatc tttattatta ttactatatg ctcttgtaaa
     9241 ataatcattc caatccttta cttttgcaga aggatttgta taattaacca ataaaaaatc
     9301 ataataaaat gctcctataa ctgggcctcc gcaaaaataa gaaggaaacc aactatcata
     9361 atcattaata tacatatgta atgcttgccc aatctgtttt aaattattca tacatactgc
     9421 ttgtcttgct ttttctctcg ctttgcttaa tgcaggtaaa agcatcgcag caagaatcgc
     9481 tattatagca actacaacaa gcaactctat caatgtgaag ccatgtggtt ttgaaaaaat
     9541 gctctttttc gccaaacggc atgtcggata cgccactctt tttggcgtag aaaaattttc
     9601 ttcataatgc acctcctttc acatttattt ttttaaataa ttttttacat gaaaaaccct
     9661 ctactatatt tacatcaaga attatttttt gaattggact tttatctcca tttattcttt
     9721 tgattaaaat ctcacttgct ttttctccta tttcttctgt cttctgttct attgttgtta
     9781 atacaggatt taaaatagat gagaaatgtg ccttatcaaa tcctatcaca gataaatcat
     9841 caggaatttt tatatccatt tcaatacatg tcttataaac accaaaagca atagggtcat
     9901 ttgtcgcaaa aatagcagtt ggtggatttt taagatgaag caattctttt gtatttgtat
     9961 aacctgactc atcatattgt aattctgtat aacttctttt tacaagcatt gggtcaaatt
    10021 caatattttc ttcttcaagt gcttttttat atccttgtaa tctctccctt actgtatttg
    10081 catttattcc aagtattatt cctattcttc tatgaccaag tgaaattaaa tactttgtcg
    10141 caatataccc tcctttaaaa ttatcagagg tgacataatc tgtctctatg tcttcaattg
    10201 ttctatctac aaatataaat ggaatccttc tttcctttaa ttcataaaag atattattgt
    10261 ctgaagaagt tctgtttacc tctgtcggtg tcattattat cccacttact tttctctcaa
    10321 taaattcttt taaaatcttt ttctctttct ctacattgtt ataacttaca cctacaatta
    10381 tatggtatcc attagaagaa aaaaccttct ctatacctgt ataaatttca gcaaaaaatg
    10441 gattttttac aatgtctgta aaaatcactc caataatttt ttcattttcc ttttctttaa
    10501 caaaaactcc cctaccctga acccctatca ataatccttc ttttattaat tctttcaatg
    10561 cctgcttaac aacaatatca cttacattat aaatttttga aagttctgaa attgatggga
    10621 ttttctctcc aactttatat tctcctttct ctattttttc ctttatatcg tcccttattt
    10681 tcttatatag tgccattttc atccttttta tacttattat aacaatatta agatttttgt
    10741 caagttttta tatgttttta attttggtct ccaaatatta aaatttcttt taatacttat
    10801 tatacttttt aatcatttat cgggttttta tatttaaatt tcatcatctc atttccttta
    10861 ctataaaaaa ataatattat caattaggta tgttttaaac taaaagtggc tattgacaaa
    10921 atttttttga atgaataaaa taaactctat gatttcattt aaaagtatag taattggtct
    10981 tattttttgt attttaatcg gaattggaga accattaaat gttctttata ttcatggttc
    11041 tgctcttgct gctgattatt caacaggtgc tgctatcttt ttattttttt tacttgttgg
    11101 catcttaaat caattcaata aaaaatttat caaaaaatta tatcttaccc ctcctgaatt
    11161 gattactgtt tacattatga tgattgttgc ttgtgccata ccttcttggg gttttacaat
    11221 gaatttaatt ggacttcttg gtggaatttt ttattatgca actcctgtaa atgaatggga
    11281 taaatttatt catccatatt tgccaaaaca tttatttccg aaggactata atgcaatatg
    11341 gtatctttat gaaggacttc caaaaaatat gaaaattcca tgggaagaat ggatattacc
    11401 tcttttcaac tggttttctt ttataattgc
//
LOCUS       DAOSAT010000059        11357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3818_length_11357_cov_4.593523, whole genome shotgun sequence.
ACCESSION   DAOSAT010000059 DAOSAT010000000
VERSION     DAOSAT010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3818_length_11357_cov_4.593523"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..990)
                     /locus_tag="PLF90_06180"
     CDS             complement(127..990)
                     /locus_tag="PLF90_06180"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOK56899.1"
                     /translation="MKKENNCSFFVPLLLFLILCILITGIKLLYKFTEKGKVEKKEEV
                     TEFYPPEIKPEVPRKFEKKKLKGKGEVAIIIDDVGWNLSIIEEVKKINQPLTLSLLPK
                     APYSKKILDELKDKNYELLLHLPLEPSPPSQCLDKGLIKTDMTDEEIKKIFEDNVNEF
                     LPYIKGINNHMGSLYTVNEEKMKILLENMKEKNLFFVDSLTNSKSCGFKIAKELGIKT
                     GKRDIFLDISPDPEEVNKKIDELIEIAKEKGNAIAIGHAKTSTINVLKNRITDFEENG
                     IKIVPVSSLLE"
     gene            complement(991..2157)
                     /locus_tag="PLF90_06185"
     CDS             complement(991..2157)
                     /locus_tag="PLF90_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOK56900.1"
                     /translation="MRKRIILVILGFLFLSIVFGGDVKVSREKKWEDNPYKNLELLID
                     ALNIVRDNYVEPVENKKLIYGALKGMLMSLDPYSAFLEPELKKELQIETKGEFEGVGM
                     EITLKDGIITVVSPIEDTPAWKSGIKEGDKIIEIDGKSTKGMTTMEAAQMLRGKKGTK
                     VTISVLREGVSNLMKFTLERDVIKIKSVKSKIYREIGYVRLTGFQENTTEDLKKTLIE
                     FNEKNLKGLILDLRNNPGGLLNVAIEVSEMFLPPNKLIVYTQGRNKKDSAKFYSKNRP
                     LWQKPIVLLVNGGSASASEILFGALKDNYQNFLSVGTKTFGKGSVQNVIPLSDGSAIK
                     LTIAKYYTPKGICIEGEGIVPDIEVKLEGEKPIIPTSEEDTQFKRALEEIKKMI"
     gene            complement(2190..3617)
                     /gene="gatB"
                     /locus_tag="PLF90_06190"
     CDS             complement(2190..3617)
                     /gene="gatB"
                     /locus_tag="PLF90_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOK56901.1"
                     /translation="MEEYEIIIGLEVHVELLTKTKMFCGCSTEFGNPPNTQICPVCTG
                     MPGVLPVINKRAIELAIKTGIVFNSNISKFCRFARKNYFYPDLPKNYQISQYEEPIAT
                     GGHLDVDGRRIHFKRIHLEEDAGKLLHSENSFFSFVDFNRSGIPLLEMVTEPDIRSPE
                     EAERFLEKLKQILEYLEISDCNMEEGSLRCDANISVRKKGDEKLGVKTEVKNMNSFKS
                     VRKALLYESKRQMEILKKGEKINQETRLWNEKEEITESMRTKEEAHDYRYFPEPDLVP
                     VEINSEWIEKIKGEIAELPDERKERFKKEYNLSDYDAGVLISQKGIADYFEICVRNYN
                     NPKIIANWIMGELLSLTKGKILNKEEIPLKVENFIEILKMIDEKKITMTVGKEIFKES
                     FMTGEKPSEIVKKRNLIQIEDESIIKNIVNEVLQENQKAIDDWKKGKEQVIGFLVGQV
                     MRKTGGKTNPNIVKEILLDSLKNLK"
     gene            complement(3619..4632)
                     /locus_tag="PLF90_06195"
     CDS             complement(3619..4632)
                     /locus_tag="PLF90_06195"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HOK56902.1"
                     /translation="MLNRNNGCRFMEFEWKGYKCIYLENEIIRVEILVDKGTDIVEFL
                     YKEKDIDFMWRSPIPVYHQSKLIQTAPTKLGSFIDYYPGGWQEIFPNGGGICEYKGAI
                     LGLHGEVALLPWDYKIIEDTKDRVSIKFSVYTYRTPFYLEKIITIKSGIPYIEIYEEV
                     FNLAGEEVDFIWGHHPAFGKPFLSENCIINIKGGKVKVVPGDGKSYTNLKQTEGNWPF
                     VEGIDGKQVDISKVPSEEDNVSDVIFISDLEEGYYEIINESLKTGFFMEFPKEVFKYL
                     WFWRIAKGSYNYPWWGRNYNIALEPFSSLPNLAEAIKRNDQLKLKENCSLKVSLKAGV
                     KKI"
     gene            complement(4616..5695)
                     /locus_tag="PLF90_06200"
     CDS             complement(4616..5695)
                     /locus_tag="PLF90_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOK56903.1"
                     /translation="MNFWKFFIIFILFLSFSSYSITYTVYHTVKKGENLSVIAKKYGK
                     TVDELKKMNNLKTSNILPGQRLVVDKKEKEEKITKEKSGNLVKEYYTVKKGDSLYKIS
                     QKCGLTITQLKKLNNLKSSSIKPGQKLVVGVKKIETVKKDINPDEIQPIVDVSKKTYY
                     KICEEDTLESISEKFGIEPEKLLQANLIKKEDLKVGQIIVIPPRNLLDEETKKDTILD
                     KQSNVRTKLIEEAFNYLGVKYKYGGESKSGLDCSALTRLVYKTIGILLPQNSSLQYKN
                     GIEIEKEEALPGDLVFFKRGSGIGHVGIYLGNDLFVHASYTLRRVVISSLSERYFKER
                     FAGFRRYLIDEETYFAKGLENVEQE"
     gene            complement(5692..6405)
                     /gene="cysE"
                     /locus_tag="PLF90_06205"
     CDS             complement(5692..6405)
                     /gene="cysE"
                     /locus_tag="PLF90_06205"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOK56904.1"
                     /translation="MFETIKEDIKVVFERDPAARNVFEVITSYPGLHAIWLHRIAHFF
                     WKKGARTFARIISHISRFLTGIEIHPGAKIGRRFFIDHGMGVVIGETSEIGNDVLIYQ
                     GVVLGGTSLKKEKRHPTIGNNVVIGAGAILLGPIKVGDGAKIGAGSVVIKDIPEGTTA
                     VGVPARIVEKREKKIDLDHDKLPDPILKIFQLILDEQEELKRRIKELETLEGLKSKID
                     EYFEEKKKEIMELLEGGIK"
     gene            complement(6398..7609)
                     /locus_tag="PLF90_06210"
     CDS             complement(6398..7609)
                     /locus_tag="PLF90_06210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOK56905.1"
                     /translation="MLREIIMPKLGETMEEGYLVSWKKNEGDKVEKGEVLFEVMSDKT
                     NFEVESPYSGYLRKILFHPSDQPIPVTTVIAYMTDTPDEPIEIKEEKKEIKVEEKKEV
                     ISVSEIKKEKVEEKEERIKISPVARRLAEEYGVDISEIKGTGPEGRIEKKDILEYIEK
                     RKEKIEKERIVSEDRGEYEVIKWTPLRKIIAKRLTESKQTIPHYYFQGKVIVDNILKI
                     KENKEKEGEKYTITDFLIFLTSRVIEEFPVIGAGVVNEEIRVYKNIDIGLAISVPEGL
                     VVASIKNANKKTIKEITEERKKLVERAREGKLTEEDIKGTNFVISNLGMYEVENFYPI
                     INPPGVAIMGVGKIEKGIFVENESPVIKNYMYISFSFDHRIIDGAYAANFYKRLKEVI
                     ENPSILLFKNV"
     gene            complement(7635..8609)
                     /locus_tag="PLF90_06215"
     CDS             complement(7635..8609)
                     /locus_tag="PLF90_06215"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOK56906.1"
                     /translation="MRTITYRQALNEGLDEEMARDPRVFLFGEDIAIYGGAYGVTAGL
                     WQKYGDERVIDTPISENAIVGTALGAALTGLRPVAEIMYIDFIGLCMDQLNNQVAKIR
                     YMFGGKCTVPLVIRTEGGAGRTLGAHHSQSLEAWLIHIPGIKVVMPSTPYDAKGLLKS
                     AIRDDNPVVFIEHKMLYNTKGEVPEEEYLIPIGVADVKKEGTDVTIITYSRMVLFSLE
                     AASILEKEGISVEVIDLRTLLPMDIETVVKSVKKTNRVVIVEEDCKTGGTGAEIGMQI
                     VENAFDYLDAPIIRVAGADVPMPKSPVLEKLAIPDVERICKGVKEVLK"
     gene            complement(8624..9583)
                     /locus_tag="PLF90_06220"
     CDS             complement(8624..9583)
                     /locus_tag="PLF90_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOK56907.1"
                     /translation="MEITKELAVKLLKQMMQIRQFEDMIMELLAKNIAEGGSHLYAGM
                     EGVAVGAISVINPDDYITSTHRGHGHAIAKDGDLKALMAEILGKKTGVCKGKGGSLHL
                     ADLSKGNLGANGIVGGGLGIATGAGLSIKLQKQNKVVLCFFGDGATNNGIFFECLNMA
                     SLWKLPVIYICENNKYAMSVSVERSHAVKDITKKAIAFDIPAENVDGQDVLAVREVVA
                     KWVNYARESKGPSFIVANTYRYYGHSRSDPRVYRTREEEKFWKERDPILIFANKMKEQ
                     QILTEEEINAIEEEVRREIEEAVDFAIKSPYPEPEELYTDLYV"
     gene            complement(9597..10631)
                     /locus_tag="PLF90_06225"
     CDS             complement(9597..10631)
                     /locus_tag="PLF90_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HOK56908.1"
                     /translation="MKGAILKDIEKLEIEDVKIPDPSENEILIKVKACAICGTDIKVF
                     HHGHKHIVFPRITGHEVSGIVEKVGKNVKEYKEGDRVAVAPAIPCGRCYYCRKGQQTM
                     CENLKAIGYHYDGGFAEYMLVPENAVLNGCVNKIPDNLSFELACLAEPLACVINGQIL
                     SRIEVGDIILILGAGPIGILHSLLAKVNGAGKVILADISEERIKEAEFTGCYLINMNK
                     TDIYEEVKKITDGHMADRVIVAVGSKQAQEISLKLVAKRGSINFFGGLPKEAPYIQFD
                     SNLLHYGEFFVVGTHGSTPLQNKIAIELLSTGRINIEKLITHKLPLEKIKDGFEIVEN
                     KKGMKVLIMP"
     gene            complement(10628..>11357)
                     /locus_tag="PLF90_06230"
     CDS             complement(10628..>11357)
                     /locus_tag="PLF90_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56909.1"
                     /translation="IDFLNKNVGFVETVISRMTSPLTEEEKKENPLLVKVEPYKILPV
                     DKNGFKGKIPEIVGFLPVSNLHQYEELKLFGHNLSHVSLAYYGYLKGYTYIWQCVEDK
                     EILNLLLNVQKEIKEALIKKYNFGKEELENYFSDLNMRFDNKLLGDTIYRVGREPLRK
                     ISKNERIVGAINLCLEYNIFPENVCFVLATALCYNYSLDRESIELQEIKKKRGIDFVL
                     EEICGIYNEKIKKRVKEIYKKVKK"
BASE COUNT         3351 a         2275 c         1218 g         4513 t
ORIGIN      
        1 acaacaatat tagcaaatat ctttttgtct tttattatcc caattcctgt ttcatcacag
       61 gatgtttcaa taccaataac tatcatttat ttttctcctt taatatttct aaactttaaa
      121 taacaatcat tctaaaagag atgaaacagg aacaatctta ataccgtttt cctcaaaatc
      181 agttatcctg ttttttaaca catttattgt agatgtttta gcatgcccaa ttgcaattgc
      241 atttcctttc tcttttgcta tttctattaa ttcatctatt tttttattta cttcttcagg
      301 gtcaggtgat atgtcaagaa aaatatctct cttacctgtt tttattccta attcttttgc
      361 aattttaaat ccacaacttt ttgaattcgt caaactatca acaaaaaata agtttttttc
      421 tttcatattt tcaagtagaa ttttcatttt ttcttcattt actgtatata gagagcccat
      481 atgattgtta atacctttaa tatagggtaa aaattcattg acattatctt caaaaatttt
      541 ttttatttct tcatctgtca tatcagtttt tattagccct ttgtcaagac attgagatgg
      601 tggtgatggt tcaagaggaa gatgtaaaag taattcataa tttttatctt ttaattcatc
      661 taaaattttt ttactgtagg gcgcttttgg taaaagtgaa agcgtaagtg gttgatttat
      721 ttttttaact tcttctatta tagataaatt ccatccaaca tcatcaatta taatagccac
      781 ttcacccttc cccttcaatt ttttcttttc aaattttctc ggtacttcag gttttatttc
      841 tggaggataa aactctgtaa cttcttcttt tttttcaact ttcccttttt ctgtaaattt
      901 ataaagaagt tttatccctg taattaaaat acacaaaatc aaaaataaaa gaagtggaac
      961 aaaaaaggaa caattattct cttttttcat ttaaatcatc ttttttattt cttcaagagc
     1021 tcttttaaac tgagtatcct cttcactcgt tggaattatt ggtttttctc cctcaagttt
     1081 aacctctatg tcaggaacaa tcccttctcc ttcaatacat attccctttg gagtataata
     1141 ttttgctatt gtcaatttta tcgctgaacc atctgaaagt ggtataacat tctgaacaga
     1201 acctttacca aaagttttag ttcccaccga aaggaaattt tgataattgt ctttaagagc
     1261 accaaacaat atttcagaag cagatgcact ccctccattt acaagaagaa ctattggctt
     1321 ttgccataaa ggtctatttt tgctgtagaa ttttgcagag tcctttttat ttcttccctg
     1381 tgtataaaca attaatttgt taggcggtaa aaacatttca ctcacttcaa tagcaacatt
     1441 taaaagtcca ccgggattat ttcttaaatc aagaattaaa ccttttaaat ttttttcatt
     1501 aaattcaatt aaagtttttt tcaaatcttc tgttgtgttt tcctgaaatc cggttaacct
     1561 cacatagcca atttctctat atatttttga ttttactgat tttattttta tgacatccct
     1621 ttctaatgta aacttcatca aatttgaaac accctctctt aaaacactta ttgttacttt
     1681 tgtacctttt ttccctctta acatttgagc cgcttccata gttgtcatac ctttggttga
     1741 ttttccatct atttctatta ttttatcgcc ctctttaatt ccggatttcc acgcaggagt
     1801 atcttcaatt ggacttacaa cagtaattat tccatcttta agtgttattt ccattcccac
     1861 accttcaaac tctccttttg tttctatctg caattctttt ttcaattcag gttctaaaaa
     1921 tgcactatat gggtcaagag acattaacat accttttaaa gcaccgtata tgagtttttt
     1981 attctccaca ggctcaacat aattatctct gactatattt aaagcatcaa taagtagttc
     2041 cagattttta tagggattat cttcccactt cttttcccga gaaaccttga cgtcgccacc
     2101 aaaaacaatt gaaaggaata aaaaacccag aattactaaa attattcttt ttctcatatt
     2161 tctccttttt tatctattta ttttaccctt tattttaaat tttttaaact gtccaataaa
     2221 atttccttta caatatttgg attcgttttt ccacctgttt ttcgcattac ttgaccaacc
     2281 agaaaaccta taacctgctc ttttcctttt ttccagtcat caattgcttt ctggttttcc
     2341 tgtaaaactt cattaacaat attttttatt atactttcgt cttctatctg aataagattt
     2401 ctctttttta caatttcaga tggtttttca cctgtcataa acgattcttt aaatatttct
     2461 ttaccaacag tcattgttat ctttttttca tcaatcattt ttaaaatttc aataaaattt
     2521 tctactttta gaggaatttc ttctttattt aatattttac cttttgttaa agataagagt
     2581 tcacccatta tccagtttgc aattattttc ggattgttat aatttcttac acatatttca
     2641 aaataatctg caataccttt ttgagaaatt aaaacacctg cgtcataatc agatagatta
     2701 tattctttct taaatctttc ttttctttca tctggaagtt ctgctatttc tccttttatt
     2761 ttttcaatcc attcactatt tatctcaaca ggaacaaggt ctggttcagg aaaatatcta
     2821 taatcatgtg cttcttcttt agtcctcata ctttcagtta tctcttcttt ctcattccac
     2881 aatctggtct cctgatttat tttttctcct ttttttagta tctccatctg ccttttactt
     2941 tcatatagta gtgcttttct aacagattta aaagaattca tatttttaac ttctgtcttt
     3001 actccaagtt tttcatctcc ttttttcctg acagaaatat ttgcatcaca tcttaaagaa
     3061 ccttcttcca tattgcaatc acttatttca agatattcaa gtatctgctt caatttttca
     3121 agaaaccttt ctgcctcttc tggacttctt atatctggtt ctgtaaccat ttcaagtaaa
     3181 ggaattccac ttctgttaaa atcaacaaat gagaaaaaac tgttttcaga atgtaataat
     3241 ttccctgcat cttcttcaag atgtattctt ttaaaatgga ttcttcttcc atctacatca
     3301 agatgacctc ctgttgcaat aggttcttca tattgagata tctgataatt ttttggtaaa
     3361 tcaggataaa aataattttt tcttgcaaat ctacaaaatt ttgaaatatt tgaattaaaa
     3421 acaatacctg tttttatggc tagttcaatt gctcttttat ttataacagg taaaactcca
     3481 ggcattcctg tacagacagg acagatttga gtatttgggg gatttccaaa ttctgttgaa
     3541 caaccacaga acatctttgt ttttgtcaaa agttcaacat gtacttcaag tccaataatt
     3601 atttcatact cttccatttt agattttttt cactcctgct tttaaactga ctttcaatga
     3661 acaattttct tttaatttta actggtcatt tctttttatt gcttctgcta aatttggtaa
     3721 acttgaaaat ggttcaagag caatattata atttcttccc caccatggat agttgtaact
     3781 cccttttgca attctccaga accatagata tttaaaaact tctttaggaa attccataaa
     3841 aaatccagtt ttcaaacttt catttattat ttcataataa ccctcttcca aatcagatat
     3901 gaaaataacg tcagaaacat tatcttcttc agaagggact ttgcttatgt caacctgttt
     3961 tccatcaatt ccttcaacaa atggccagtt tccctctgtc tgttttaaat ttgtatatga
     4021 ttttccgtca ccaggaacaa cttttacttt accaccttta atatttataa tacaattttc
     4081 tgataaaaat ggtttgccaa aagcaggatg atgtccccag ataaaatcaa cttcttcacc
     4141 tgcaagatta aaaacttctt catatatttc tatatatgga attccgcttt taatcgtaat
     4201 tattttttca agataaaatg gagttctata tgtataaaca gaaaatttaa tagaaactct
     4261 gtcttttgta tcttcaatta ttttataatc ccatggcagt aaagcaacct ctccatgaag
     4321 ccctaaaatc gcacctttat actcacaaat tccgcctcca ttcgggaata tctcctgcca
     4381 tccaccggga tagtaatcaa taaaactacc taattttgtc ggtgctgtct gaattaactt
     4441 tgattgatga taaacaggta ttggacttct ccacataaaa tcaatatctt tttccttata
     4501 aagaaattca actatatctg ttcctttatc aacaagaatt tcaactctta taatttcatt
     4561 ttcaaggtaa atgcatttat aacccttcca ttcaaactcc ataaatctac aaccgttatt
     4621 cctgttcaac attttcaagc ccttttgcaa aatatgtttc ctcatcaatc aaatatcttc
     4681 tgaatcctgc aaatctttct ttaaaatatc tttcacttaa cgaacttata acaactcttc
     4741 tcaatgtata acttgcatga acaaaaaggt catttccaag atatattcca acatgtccaa
     4801 ttccacttcc tcttttaaag aaaaccaaat ctccaggtag tgcttcctct ttctctattt
     4861 caattccatt tttgtattgt agagaagaat tctgtggcaa taatattcct atcgtcttat
     4921 acacaagtcg ggtcaatgca gaacaatcca aaccactttt actttcacca ccatatttgt
     4981 atttaactcc aagataatta aatgcttcct ctatcaattt tgttctgaca ttgctttgtt
     5041 tatctaaaat tgtatccttt tttgtttctt catcaagtaa atttcttggt ggaataacaa
     5101 taatctgacc tacttttaaa tcttcttttt taataagatt tgcctgtaga agtttttcag
     5161 gttcaatacc aaatttctca cttattgatt ctaaagtatc ttcttcacag attttatagt
     5221 atgttttttt tgaaacatcc actattggct gtatttcatc tggattaata tcttttttca
     5281 ctgtctctat tttctttact cctaccacaa gtttctgccc tggtttaatt gaagaacttt
     5341 ttaaattatt caattttttt aattgtgtaa ttgtaagacc acatttttgg gatattttat
     5401 ataaagaatc gcctttttta acagtataat attctttaac taaattccca cttttttctt
     5461 tggtaatttt ttcttctttt tcttttttat caacaactaa tctctgtccg ggtaaaatat
     5521 ttgatgtttt taaattattc atttttttca gttcgtcaac tgtttttcca tatttcttag
     5581 caattacaga aagattttct ccttttttta cagtatgata aacagtatat gtaatgctat
     5641 aagaggaaaa agataaaaac aaaatgaaaa ttatgaaaaa tttccagaaa ttcatttaat
     5701 acctccttct aaaagttcca taatttcttt tttcttttct tcaaaatact catctatttt
     5761 tgattttaaa ccttcaagtg tttcaagttc ttttattctt cttttcaatt cttcctgttc
     5821 atcaagaatt aactgaaaaa tttttaatat tgggtctgga agtttatcgt ggtcaaggtc
     5881 tatctttttt tctctttttt ctacgattct tgcaggcact ccaacagcag tagtcccttc
     5941 aggtatatcc tttataacta cactacctgc tcctattttt gctccatctc caacctttat
     6001 tggaccgagt aaaattgctc ctgctcctat aactacatta tttccaattg ttggatgtct
     6061 tttctctttt tttaaagaag tgccaccaag aacaactccc tgatatatta aaacatcatt
     6121 tcctatttct gatgtttcac caataacaac tcccattcca tggtcaataa aaaaccttct
     6181 acctattttt gctccaggat gtatttctat tcctgttaaa aatctgctta tatgggaaat
     6241 aattcttgca aatgttcttg cccctttttt ccagaaaaaa tgggcaattc tgtgaagcca
     6301 tatagcatgt aatcctggat aacttgtaat tacttcaaaa acatttcttg ctgccgggtc
     6361 tctttcaaaa acaactttaa tatcttcttt tattgtctca aacatttttg aaaagtaaga
     6421 ttgatggatt ttcaatcact tcttttaatc ttttataaaa atttgctgca tatgcaccgt
     6481 caataattct gtggtcaaaa gaaaatgaaa tatacatata attttttata acaggactct
     6541 cattttcaac aaaaataccc ttttcaattt tcccaacacc cataattgca acaccaggtg
     6601 gatttattat tggataaaaa ttttcaacct catacatacc aagatttgaa ataacaaagt
     6661 ttgtcccttt tatatcctct tcagttaatt tcccttctct tgctctttca acaagttttt
     6721 ttctttcctc tgtaatttct tttattgttt ttttattcgc attttttatt gaagcaacaa
     6781 caagaccttc gggaacactt atagcaagtc caatatcaat atttttataa acccttattt
     6841 cttcattcac aactcctgct ccaataactg gaaattcctc aatgactctt gatgtcaaaa
     6901 aaatcaaaaa atctgtaatt gtatattttt ctccttcttt ttctttgttt tcttttattt
     6961 taagaatatt atcaactatt acctttccct gaaaataata atgaggaata gtttgtttac
     7021 tttccgtaag tcgttttgca attatctttc ttaaaggggt ccactttatt acttcatatt
     7081 ctcccctatc ttctgaaact atcctttctt tttcaatttt ctcttttctt ttttcaatgt
     7141 attcaagtat gtctttcttt tctatccttc cctcaggtcc tgtgcctttt atttcactta
     7201 tatcaactcc atattcctct gccaaccttc ttgcaactgg actgattttt attctttctt
     7261 ctttttcttc aactttttcc ttttttattt cagaaacaga gataacttct ttcttttctt
     7321 caacctttat ttcttttttt tcttccttaa tttctattgg ttcatcaggt gtatctgtca
     7381 tatatgcaat aacagttgta acaggaattg gctggtcaga aggatgaaaa agaatcttcc
     7441 ttaaataacc ggaatacgga ctttcaactt caaaatttgt tttatcactc ataacttcaa
     7501 aaaggacttc tcctttttca accttatctc cttcattctt tttccaactg accaaatatc
     7561 cttcttccat tgtctcacca agtttcggca taattatctc tcttaacatt actcaactcc
     7621 tttttaactc tatttcattt taacacttct tttactcctt tacaaattct ttcaacatca
     7681 gggatggcaa gtttttcaag aacaggactt tttggcatag gaacatcagc acctgcaaca
     7741 cgtataattg gggcatcaag gtaatcaaag gcattttcaa ctatctgcat acctatttca
     7801 gcacctgttc cacctgtctt acaatcctcc tcaactataa caaccctgtt tgtcttttta
     7861 actgatttaa caactgtttc aatatccata ggtaaaagtg tccttaaatc aataacctca
     7921 acacttattc cttctttttc taaaatactg gctgcttcaa gtgagaataa aaccattctt
     7981 gaataagtta taattgttac atcagtacct tcttttttta catcggcaac accaattgga
     8041 ataagatatt cttcttcagg aacttctccc tttgtattat aaagcatttt gtgttcaata
     8101 aatacgactg gattatcatc tcttattgcc gattttaata aaccttttgc atcataaggg
     8161 gttgaaggca taacaacttt aattccaggt atatgtatca gccatgcctc taaactctgt
     8221 gagtgatgtg ctccaagagt tctgcctgct ccaccttctg ttcttattac aagaggaact
     8281 gtacattttc caccaaacat atatcttatt tttgcaacct gattattgag ttggtccata
     8341 caaagtccta taaaatcaat atacataatt tcagcaacag gtcttaaacc ggttaaagca
     8401 gcaccaagag cagttccaac aatagcattt tctgaaattg gtgtatctat aactctttca
     8461 tctccgtatt tctgccaaag tcccgctgta acaccgtagg caccaccata aattgctata
     8521 tcttctccaa atagaaaaac tctcgggtcc cttgccattt cttcatcaag accctcatta
     8581 agtgcctgtc tgtatgttat agttctcatt ttttaactcc tttttaaaca tataaatcag
     8641 tataaagttc ttctggttct ggatatggac tttttattgc aaaatcaacc gcttcttcta
     8701 tttctcttct tacctcttct tcaattgcat ttatttcttc ttcagttaaa atctgctgtt
     8761 ccttcatttt atttgcaaaa attaaaatcg ggtccctttc tttccagaat ttttcttcct
     8821 ctctggttct gtaaactctt gggtcacttc tactgtgtcc ataatatcta tatgtatttg
     8881 caactataaa acttggtcct ttactttctc tcgcataatt tacccatttt gccacaactt
     8941 ctcttacagc aagaacatcc tgtccatcca cattttcagc aggtatatca aaagcaattg
     9001 ctttttttgt tatatccttt acagcatgag acctttcaac agatacactc attgcatatt
     9061 tattattttc acatatataa ataaccggta atttccataa agaagccata tttaaacatt
     9121 caaaaaatat tccattattt gttgctccat ctccaaaaaa gcataaaacc actttattct
     9181 gtttctgaag tttaattgaa agacctgccc ctgtcgctat tccaagccct cctcctacta
     9241 ttccatttgc tcctaaattt ccttttgaaa ggtcagcaag atgtagtgac ccaccttttc
     9301 ctttacaaac acctgttttt ttaccaagaa tttctgccat aagtgctttt aaatcaccat
     9361 cttttgcaat agcatgacca tgccctctgt gagtacttgt aatatagtca tccggattta
     9421 taactgaaat tgctccaact gcaacacctt ccattcctgc ataaagatgt gaaccacctt
     9481 ctgcaatatt tttggcaaga agttccatta tcatatcttc aaactgcctt atttgcatca
     9541 tctgttttaa aagttttaca gcaagttctt ttgtaatttc catcttattc tcctttttaa
     9601 ggcattatta aaactttcat tcccttttta ttttcaacta tttcaaaccc atcttttatc
     9661 ttttcaagag gtaatttatg agttataagt ttttctatat ttatccttcc tgttgataaa
     9721 agttcaattg caattttatt ttgcagaggc gttgaaccat gcgtacccac aacaaaaaat
     9781 tctccataat ggagtaaatt actatcaaat tgaatatatg gtgcttcttt aggtaacccg
     9841 ccaaaaaaat ttattgaccc tctttttgca acaagtttca aagaaatttc ctgtgcctgc
     9901 ttacttccaa ctgcaacaat aactctatct gccatatgac catctgtaat ctttttaact
     9961 tcttcataaa tatctgtttt gttcatattt ataagataac aacctgtaaa ttctgcctct
    10021 ttaattcttt cctcagaaat atcagcaagg attacttttc ctgcaccatt aacttttgct
    10081 aaaagtgaat gtaaaattcc aataggacct gctccaagaa ttaaaattat atcaccaact
    10141 tcaatcctac ttaaaatttg accatttata acacatgcaa gtggttcagc aaggcaagca
    10201 agttcaaaag aaagattatc aggtatttta ttaacgcaac cattcaatac agcattttct
    10261 ggaacaagca tatattcagc aaatccacca tcataatgat aaccaattgc tttcaagttt
    10321 tcacacattg tttgctgtcc ttttctgcaa taataacatc ttccacatgg tatcgcagga
    10381 gcaactgcaa ctctatctcc ctccttatat tctttaacat tcttcccaac cttctcaact
    10441 attccactta cttcatgacc tgttattctt ggaaagacaa tatgcttatg tccatgatgg
    10501 aaaactttaa tatcagttcc acatatagca catgctttta ctttaatcag aatttcattt
    10561 tctgatgggt cgggtatttt tacatcttca atttcaagtt tttcaatgtc ttttaaaatt
    10621 gcacctttca tttctttacc tttttgtaaa tttccttaac tctttttttt atcttttcat
    10681 tataaatacc acatatttct tccaaaacaa aatcaattcc tctcttcttc ttaatttcct
    10741 gcaattctat actttctcta tcaagtgaat aattatagca taaagcagtt gcaaggacaa
    10801 aacatacatt ttctggaaaa atattatatt caagacataa attaattgca ccaacaattc
    10861 tttcattttt actgattttc ctcaaaggtt ctcttccaac tctgtaaata gtatctccaa
    10921 gaagtttatt atcaaatctc atattaaggt cagaaaaata attttcaagt tcttctttgc
    10981 caaaattata tttttttata agtgcctcct taatttcctt ttggacattt aaaagtagat
    11041 ttaaaatttc cttatcctct acacattgcc aaatataagt ataacctttt aggtaaccat
    11101 aataggcaag agatacatga gaaaggttat ggccaaaaag ttttagttct tcatattgat
    11161 gtaaattaga aacaggtaaa aaaccaacta tttcaggaat ttttccttta aatccatttt
    11221 tatcaacagg gagaatttta tatggttcaa ccttaacaag caatggattt tctttttttt
    11281 cttcttctgt taatggagaa gtcattctac ttataactgt ttcaacaaat ccaacattct
    11341 tatttaaaaa atcaatc
//
LOCUS       DAOSAT010000060        11317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3830_length_11317_cov_5.590392, whole genome shotgun sequence.
ACCESSION   DAOSAT010000060 DAOSAT010000000
VERSION     DAOSAT010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3830_length_11317_cov_5.590392"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLF90_06235"
     CDS             <1..217
                     /locus_tag="PLF90_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK56910.1"
                     /translation="KIQKRLLTIKEASEYLGISEKGLYNMVWRKEIPFVKIRKRVRFD
                     IEDIKEWIERNKIKDIESEFQKVLDKI"
     gene            242..1330
                     /locus_tag="PLF90_06240"
     CDS             242..1330
                     /locus_tag="PLF90_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOK56911.1"
                     /translation="MGRIYLRGKTWYIYTCYRGKIIRKAIGKDKKLANAVLKKKEIEL
                     IENKHLDIKKENKIKFSQLSKEFLEKYSKVNKRSWKRDQLSISHIEKFLGNKYIYEIK
                     EKDIEEYKKTRLETGIKHSTLNRELACLKTILNKAKEWGYIDKIPKIKLFKENNQRVR
                     YLTDDEEEKLLKIAPEPLKSIIIVGLNTGMRQGEILNLRWKDIDLKEGIIIIGDSKSK
                     EKRIIPMNETLKNIFINLLTIGGKEYIFENRNGERYSGSYITHWFERIVKQASIKDFR
                     FHDLRHTFASRLVMKGVSVKVVQELLGHKDYRMSLRYSHLSPEVKKQAVEILDKDKNL
                     AQILHNEEIDKNEKLGKIIGNQRIGGVA"
     gene            1316..1409
                     /locus_tag="PLF90_06245"
     tRNA            1316..1409
                     /locus_tag="PLF90_06245"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1350..1352,aa:Ser,seq:gct)
     gene            complement(1526..2122)
                     /locus_tag="PLF90_06250"
     CDS             complement(1526..2122)
                     /locus_tag="PLF90_06250"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HOK56912.1"
                     /translation="MFQIFNIIGLITFSFSGAIKGISKKFDLFGIIVLGCITALGGGV
                     IRDILVNQLPKMLIDPSYIGFSLLGVLLAILLRKKITPIVEKIFLISDAIGLASFTAT
                     GCIIGKESSLGLVGIILLGLSTAIGGGIIRDILSGDIPLVLQKEFYASCSILGGFIFW
                     LCLIFGFPISFSTFICVIIVFLTRIMAIKYNWHLPHFS"
     gene            complement(2125..2694)
                     /locus_tag="PLF90_06255"
     CDS             complement(2125..2694)
                     /locus_tag="PLF90_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOK56913.1"
                     /translation="MIKKGFTIMEILIAITILVIIFTLVTVMYVRGSRIRNVVVAYNE
                     VGEVLTHMTDVLVNGRQNFIGRDIPGLKFAKGIDSNSNQHTLIYKDTSENLYTFEIKI
                     NTGTSTDTSLYFEDKPLDPNNKIVLVSGSGFEYYYPDGTKKDPDSTATETVTVVKIIL
                     HGKSNLPVMKNMNPIKMETLVRLKNNPSF"
     gene            complement(2708..3184)
                     /locus_tag="PLF90_06260"
     CDS             complement(2708..3184)
                     /locus_tag="PLF90_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOK56914.1"
                     /translation="MVNKNGLTLLEVLIAVFLMAIVIAGGLLLISGNLNVIKKANELT
                     IATALLQYTIEDIKNIDFPPVYYDRQGNFGDEVETESIVDVNLYTDYTPVFYKNDYRI
                     IRFVQGLDSSGNIISNFNETMYDNAMLLKITIYILRKRGNQVLLKEVVYRSRDGLY"
     gene            complement(3203..3418)
                     /locus_tag="PLF90_06265"
     CDS             complement(3203..3418)
                     /locus_tag="PLF90_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOK56915.1"
                     /translation="MKKVIKFDELVRKFRKLGYEGPFSGGKHLFMKKGSKKVHIPNPH
                     KSKDIHISLLKEILKCAGIDEKEWEDA"
     gene            complement(3415..3684)
                     /locus_tag="PLF90_06270"
     CDS             complement(3415..3684)
                     /locus_tag="PLF90_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOK56916.1"
                     /translation="MVKKEEKMLMEYIEKALKKAEYKKIEDGTWFGTIKNFEGVWANG
                     KTIEETRRQLIEVLEEWLLLKIKDGDEIPEIDGEKIEIKRVPSKI"
     gene            complement(3678..6335)
                     /locus_tag="PLF90_06275"
     CDS             complement(3678..6335)
                     /locus_tag="PLF90_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56917.1"
                     /translation="MEKKRNKGVALIAVIVMIVLFSSLIMVVVLSSTMSIKRAYFYHD
                     RTKALEIAENAIQDVFNWMNYRAYDTEYYPSWYANNQPPDSPTKVYFTGTTFSPSPVK
                     VNAPYIPEKGECIVEFFDDNGENKDTITAIGKYRGRTAKLSVKVRGENGYDNNTHKNF
                     ALRLGNIETFQVDINFEPIGANPSIQYPDKGEVFGDRGNGYKYGWSEDRTGYVYDRDI
                     LPNSYQYLDNQYLDTLAIMNGAKWEIEVPNWYYSVTVDCGDPMDPECDDYNHDLIIEG
                     QLSHISSPNFSVSVLRNPGPPPVYDVYWYKRHIVDVTVNDGRLTIEPVDTSNNGPRIC
                     HIQIRPNSGVNWATPEAFNKHLVYSNTVSGNTGSNQILVNGNITYNNWQANLNYKDES
                     KKTFTHTSSFSIPRNSASISIPVATTLQQPNFTPTPTMRFKDSNGSTPYDPATGYNSY
                     TDLTNINGGNDPYNVSGTSEYYTFSNLSNLSSGTYFFEKTDSKTQLQIKFTPNSNPNI
                     PSGIILKAGAIDSGEAADIIIDVNLDSSTSILGQLMAERNIEITGSIPVSIGSSNQYT
                     IAARDTVKISPSGNLTINGNIYGGKKVYIQANNNTITINGNICADNQSENSEIEIRGT
                     GTININGKIYAKTKITIDGTGLNLTISDEVNCPGEVIIQGSGNVTINKDLKGGLQTGG
                     SVNLTVNGSVFSNTANIPINIGGTGNININGVIAGVGVITISNSKLTVDARNTNYAGA
                     ICWDGNGTITISSSPAIYLGEHQYSAIFVDSGTGDVNINARINPNYYLPTSQSVSNQM
                     AIINKSSSNNININAEVVGPVYSRTNITVGTNGKVKGAIITDGTLTLNGGEIIYDSAP
                     FKNNTEVYKGFTGGRRKYLPVIGSWRIEW"
     gene            6424..7395
                     /locus_tag="PLF90_06280"
     CDS             6424..7395
                     /locus_tag="PLF90_06280"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOK56918.1"
                     /translation="MGKILVTGACGFIGWKVSEKLLEKGEEVLGIDDLNDYYDVKLKK
                     WRLDKLQKNKNFSFVKLDISRENIKYIVKEAKPDAIINLAARAGVRASIENPEIYYQT
                     NVIGNLNLLECAKDFKIKKFILASTSSLYAGCNTPFKEDLPVNNPISPYATSKKAAEL
                     TCYTYHYLFGIDVVIFRYFTVYGPCGRPDMSIFKFIKLIDEEKEITVYGDGTQSRDFT
                     YIDDIAEGTISGLNLSGFHVINLGNSNPHQLNEIIKLIEEKLNKKAKIIYAPFHKADI
                     KATYADITKAKNLLNWTPKIDINEGIERTVNWYIENRDWLKDVILKD"
     gene            7398..8324
                     /locus_tag="PLF90_06285"
     CDS             7398..8324
                     /locus_tag="PLF90_06285"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOK56919.1"
                     /translation="MSKILVTGGAGFIGSHTVDRLVKERNEVIVIDNLSTGSLENLNE
                     EVIFYNFDIREKEKLKEIFDNYYFDYIFHFAAQINVRKGEEDPIFDVDVNIGGIINIL
                     ECAKEKPPKKIIFSSTGGVMYGLTDIFPSPETIEPFPICVYGISKLTGEKLLYAYWKK
                     YGINYVALRYGNVYGPRQNYLSEAGVIAIFISRILDGKECIIFGDGNQTRDFIFIDDV
                     VEANVLFMKNETTGVFNVGTGIETSVNRIFEIIKENIGKNCKKVYGEERKGELKRSCL
                     SIEKIKSQTNWAPKYKIEEGIKKTIEWFLSKR"
     gene            complement(8330..9049)
                     /locus_tag="PLF90_06290"
     CDS             complement(8330..9049)
                     /locus_tag="PLF90_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOK56920.1"
                     /translation="MKTKRIKKGGFTLIELLVVISIISMLAGQILPSLSTAREKGRQA
                     NCINNLHQIGLAIEMYYQDYDEYPTWLSLLCKSDTPGIGSNYLGTGKIFLCLTDMNKG
                     NKGHGNPSFPEICDIPLSKVQNSYPSYTPDTFDQNAYAHRNPSVEACSYSYEFAPTHC
                     KWFESTHNQTDIAQADVNNDGVVSWKEAKVWQSNHGYGGQVPIVRCFWHYYNHKQKIL
                     NLAFRDYNVFISQPEWESSSY"
     gene            complement(9046..9735)
                     /locus_tag="PLF90_06295"
     CDS             complement(9046..9735)
                     /locus_tag="PLF90_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56921.1"
                     /translation="MKKVIEFYKGLNKQNRIIFWVCVGVIFLTILRVSIWSAKKDKTP
                     TAKIFIKQIKSPNKNEKIYAIYNAGNLKIKEALPDVEDVFQKENDEEIKRVCAWSIGQ
                     IDFNKLLTYLDSSDKKIKEITFETILKIDKKNIDYLIERFDKEDTDTKFKILDYMNSP
                     EYQEKLLKIVENEKEEIPVRKKALEILKEKGKPEEIETPLWALYYNEQNDEMKNYVYQ
                     IIKEIQKRGKK"
     gene            complement(9782..10636)
                     /locus_tag="PLF90_06300"
     CDS             complement(9782..10636)
                     /locus_tag="PLF90_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56922.1"
                     /translation="MEKYICGIDENGFGPVLGPLVITGILTSEDIKIPDYIKDSKIFY
                     KNKKDLKKIEEIAVISFYMVENRTPSSPYEIFKRFSYKKCAFEENLCEKNIPLFFESE
                     NIKEIIKRYSDLLKEKDKIKRIEVKTVCPYFFNDFVKKKKSKFLLNLFNFCEIIKEME
                     NYKNIKFFCGKIGGTIYYRNFLKYFLPDYEIYALKETDDFSEYKISKKNLSFELGFYK
                     NVENISPVASFSSIIGKYMREIIMKSIIKSLNMDEDISGYRDRKTKKFIEKINFSKFR
                     KECIIRIS"
     gene            10709..11116
                     /locus_tag="PLF90_06305"
     CDS             10709..11116
                     /locus_tag="PLF90_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOK56923.1"
                     /translation="MRFKRHCEIVKGEINLTPMVDVVFQLIIFFMLSSSFIMQPGIKI
                     KLPKAETTDIIKQREIFVNITEEGKIYFKGKVVNLSEFEKELKEELKNRKENIILIVK
                     ADKDTKHGIVVSVMDKARSCGIEKIAVATMPEF"
     gene            11118..>11317
                     /locus_tag="PLF90_06310"
     CDS             11118..>11317
                     /locus_tag="PLF90_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56924.1"
                     /translation="MRTNSIFLKAIFISFSIHILGLSLFSIVLPGPLKKIKPIEVVIY
                     PSLETRQIRENINFVSKKIEEN"
BASE COUNT         3908 a         1684 c         1689 g         4036 t
ORIGIN      
        1 aaaaatacaa aaaagattac taacaattaa agaagcaagt gaatatcttg gaataagtga
       61 gaaaggattg tataatatgg tttggagaaa agaaataccc tttgttaaaa ttaggaaaag
      121 agttagattt gatatagaag acattaagga gtggattgaa agaaataaaa ttaaagatat
      181 agaaagtgaa tttcaaaaag ttctggacaa aatttgatga aaaatattat aataaataaa
      241 aatgggcaga atatatttaa gaggaaaaac ttggtatatt tatacctgtt atagagggaa
      301 aataataaga aaagcaatag gaaaagataa aaaacttgca aacgctgtat taaagaaaaa
      361 agagatagaa ctaattgaaa ataaacatct tgatataaaa aaagagaaca aaattaaatt
      421 ttcacaatta agtaaagaat tccttgaaaa atactcaaaa gttaataaaa gaagttggaa
      481 aagagaccaa ttaagtatat cccatataga aaaattttta gggaataaat atatctatga
      541 aataaaagaa aaagatattg aggaatataa aaaaaccaga ttagaaacag gaattaaaca
      601 ttctacccta aatagagaac ttgcttgttt aaaaacaatt ttaaataaag caaaagaatg
      661 gggatatatt gacaagatac caaaaataaa gttattcaag gaaaacaatc agagagtaag
      721 atatttaact gatgatgaag aagaaaaatt attaaaaata gcaccagaac cattaaaaag
      781 cataataatt gtaggattaa atactggaat gaggcaggga gagattttaa atttaagatg
      841 gaaagatatt gatttaaaag aaggcataat tataataggt gatagtaaaa gtaaggaaaa
      901 gagaataata ccaatgaatg aaactttaaa aaatatcttt ataaatcttt taaccattgg
      961 aggtaaagaa tatatatttg aaaatcggaa tggagaaaga tattctggaa gttatataac
     1021 ccattggttt gagagaatag taaaacaggc aagtataaaa gattttagat ttcacgattt
     1081 aagacatact tttgcaagta gattagtaat gaaaggagta agtgtgaaag tagttcagga
     1141 attattagga cacaaagatt atagaatgag tttaagatat agccatttat ctccagaagt
     1201 aaaaaaacaa gcagtagaaa ttctggataa agataaaaat cttgcacaaa tcttgcacaa
     1261 tgaagaaatt gacaaaaacg aaaaactggg taaaatcatt ggaaatcaaa gaataggagg
     1321 ggtagcatag tggtctaatg cggcggattg ctaatccgtt gtgtcgtcga aaggcggcac
     1381 cgtgggttca aatcccaccc cctccgccac ttatctcttt tctcaggagg gggtgcttcc
     1441 cccttcctga aatagttctc cccccacgac gaaaaccata ggggacagtc ctttccgaaa
     1501 agataaataa tctccaccag aatttttaag aaaaatgagg aagatgccag ttatatttga
     1561 tagccattat tcttgttaag aaaacaataa ttacgcaaat aaaggtagaa aaagaaattg
     1621 ggaatccaaa tattaagcat aaccagaaga taaagccccc taaaattgaa caacttgcat
     1681 aaaattcctt ttgtaaaaca agaggtatat ctcctgaaag aatatcccta ataatccctc
     1741 ccccaattgc agttgaaagt ccaagcaaaa ttataccaac taatccaagc gaactttctt
     1801 ttcctatgat acagccagtc gcagtaaaag aagcaagccc aatagcatct gatatcaaaa
     1861 aaatcttctc aacaataggt gtaattttct ttcttaataa aattgccagt aaaacaccta
     1921 aaagagaaaa gccaatataa gatggatcta ttaacatttt tgggagttga tttacaagaa
     1981 tatctcttat aactccacca cctaatgcag taatacatcc aaggacaatt attccaaata
     2041 aatcaaattt ttttgaaatc cctttaattg ctcctgaaaa agaaaaagta ataagaccta
     2101 taatgttgaa aatttgaaac atttttaaaa tgagggatta tttttaagtc gaacaagagt
     2161 ttccattttt ataggattca tatttttcat tacaggaaga tttgattttc catgaagaat
     2221 tattttgaca actgttactg tttctgttgc agtactgtca gggtcttttt ttgtgccatc
     2281 aggataataa tattcaaatc cagaaccact tacaagaaca attttattat tagggtcaag
     2341 gggcttatct tcaaagtata aacttgtatc agtagaagtt cctgtgttta ttttaatttc
     2401 aaaggtatat aaattttcag aagtatcttt gtaaataaga gtgtgctggt tagaattgct
     2461 atctattcct tttgcaaatt ttaatcctgg tatatctctt cctataaaat tctgtcttcc
     2521 atttacaaga acatctgtca tatgggttaa aacttctcca acttcattat atgcaacaac
     2581 tacattcctt attcttgaac ccctgacata cataacagta acaagggtaa aaattataac
     2641 gaggattgta attgctatta aaatttccat aattgtgaac ccttttttaa tcatttttcc
     2701 acctttttta atataaacca tctcttgacc tataaactac ttcttttaat aatacctgat
     2761 ttcccctttt cctcaaaata taaattgtaa tttttaaaag catggcatta tcatacattg
     2821 tctcattaaa atttgaaata atattcccag aactatctaa accttgaaca aatcttataa
     2881 tcctataatc atttttgtaa aaaacaggtg tataatcagt atataaattt acatctacaa
     2941 ttgattctgt ttcaacttca tctccaaaat taccttgcct atcataataa actggtggaa
     3001 aatcaatatt tttaatatct tcaattgtat attgtaataa agcagttgct atagttaatt
     3061 catttgcctt ttttataaca tttaaatttc ctgaaattaa aagaagtcca ccagcaataa
     3121 caatagccat aagaaaaact gcaatcaaaa cttcaagtag agttaatcca tttttattta
     3181 ccattcaatt ctccaacttc tcttatgcat cttcccattc cttttcatca attccagcac
     3241 attttaaaat ctctttaagc aaacttatat gaatatcttt acttttatga ggattgggta
     3301 tgtgaacctt cttacttcct tttttcataa ataaatgttt cccacctgaa aagggccctt
     3361 cataacctaa tttcctaaat tttctcacaa gttcatcaaa ttttattact tttttcatat
     3421 cttacttgga actcttttta tttcaatttt ttcaccgtct atttccggaa tttcatctcc
     3481 atcttttatt ttcagtaaaa gccattcttc taatacttct ataagttgtc ttcttgtttc
     3541 ttcaatagtt tttccattag cccaaacacc ttcaaaattc tttattgtac caaaccaggt
     3601 tccatcttca atttttttat attctgcttt ttttaacgct ttttcaatat actccattaa
     3661 cattttttcc tccttcttca ccattcaatt ctccaacttc ctataactgg taaatatttt
     3721 cttctgccac ctgtaaagcc cttataaact tctgtattat tcttaaaagg agcgctatca
     3781 taaattattt ctccaccatt taaagtaaga gtcccgtctg ttattattgc tccttttact
     3841 tttccgtttg ttcccactgt tatatttgtt cttgaataca caggtccaac cacttctgca
     3901 ttaatgttta tattattaga acttgatttg tttattatgg ccatctgatt tgaaactgat
     3961 tgagaagtgg gtagataata gtttggattt atacgggcat ttatatttac atcacctgtt
     4021 ccagaatcaa caaaaattgc tgaatattga tgctcgccga gataaattgc tggagaggaa
     4081 gatattgtta ttgttccatt tccatcccag catatagcac cagcataatt tgtatttctt
     4141 gcgtcaactg ttaattttga atttgaaatt gtaataacac ccactcctgc tataactcca
     4201 tttatgttga tatttccagt tcctccaata tttataggta tatttgcggt attagaaaat
     4261 acactgccgt ttacagttaa atttactgaa ccacctgttt gaagtccacc ttttaaatct
     4321 ttattaattg ttacatttcc acttccctga attattactt caccagggca gttaacttca
     4381 tcgcttattg ttaaatttaa acctgttcca tctattgtta ttttagtttt agcatagatt
     4441 tttccattta tatttattgt tcctgttcct cttatttcta tttcactatt ttcactttga
     4501 ttatctgcac agatatttcc atttattgtg attgtattgt tatttgcctg aatataaact
     4561 ttttttccac cataaatatt tccatttatt gtaagatttc ctgatggact gattttaact
     4621 gtatctcttg cagcaattgt atattgattt gaagaaccga tggaaacagg gatacttcca
     4681 gttatctcaa tatttctttc agccataagt tgaccgagta ttgaggtaga tgaatcaaga
     4741 ttaacatcaa ttattatatc tgctgcttca cctgaatcaa ttgctcctgc ttttaggatt
     4801 attcctgaag gaatgttggg attgctgttt ggtgtaaatt ttatctgtaa ttgtgtttta
     4861 ctatctgttt tttcaaaaaa ataagtacct gatgaaagat tacttaaatt tgaaaaggta
     4921 taatattcac ttgttcctga aacattataa gggtcatttc caccattaat atttgttaag
     4981 tcagtataag aattataacc tgttgcaggg tcataaggag tactgccatt gctatcctta
     5041 aatctcattg taggagttgg tgtaaaattt ggctgttgta aagttgttgc tactggaata
     5101 cttatacttg ctgaatttct cggaattgaa aaagaactgg tatgtgtaaa agttttttta
     5161 ctttcatctt tataatttaa atttgcctgc cagttattat aagttatatt tccattaaca
     5221 agaatttgat ttgaccctgt atttcctgac acagtatttg aatatacaag atgtttatta
     5281 aatgcttctg gtgttgccca gtttacacct gaatttggtc ttatttgaat atgacatatt
     5341 ctcggaccat tattagatgt atctactggt tcaattgtta atctcccatc atttactgtg
     5401 acatctacaa tgtgcctttt ataccagtaa acatcgtata ctggaggtgg tccagggttt
     5461 cttaaaacac taacagaaaa attaggtgaa gaaatgtgac ttaattgccc ttctataatt
     5521 aaatcatggt tataatcatc acactcaggg tccatagggt ctccacaatc aacggtaaca
     5581 ctataatacc agttaggaac ttctatctcc cattttgctc cattcattat cgcaagggta
     5641 tcaagatact gattatcaag atactgataa gagttgggta atatatccct gtcatataca
     5701 taaccagttc tatcctcact ccagccatat ttataaccat tccctctatc tccaaaaact
     5761 tctcctttat caggatattg aatggatgga ttagcaccta ttggctcaaa atttatatca
     5821 acttgaaatg tctctatatt ccccaatctt aaagcaaagt ttttatgtgt attattatca
     5881 tatccatttt ccccccttac cttcacactt aattttgctg ttctccccct gtatttacct
     5941 atagctgtta ttgtatcttt attctcccca ttatcatcaa aaaattcaac tatacattct
     6001 cctttttctg gaatataagg ggcatttact tttacaggac ttggggaaaa agttgttcct
     6061 gtaaagtaaa cttttgttgg agaatcaggt ggttgattgt ttgcatacca ggaaggatag
     6121 tattctgtat cataggcacg gtaattcatc cagttaaata catcctgtat tgcgttttct
     6181 gctatttcaa gtgcttttgt tctatcatga taaaaataag ccctttttat tgacattgta
     6241 gatgaaagga caacgaccat tataagggaa gaaaaaagaa ctatcattac aattacagca
     6301 attaatgcta cacctttatt tctttttttt tccacagttt gattttacct tgttttttgt
     6361 aggtgtgtca agttaaatta attttttgat ttgacttttt aaatttatga tataatttaa
     6421 agcatgggaa aaattttagt tacaggtgca tgtggtttta ttggatggaa ggtttcggaa
     6481 aagttattgg aaaaaggaga agaagttctt ggtattgatg atttgaatga ttattatgat
     6541 gtaaaattga aaaagtggcg actggataaa ttacagaaaa ataaaaattt ctcatttgta
     6601 aaacttgata tttcaaggga aaatattaaa tatattgtaa aggaagcaaa gccggatgca
     6661 ataataaatc ttgctgctcg tgctggagtg agagcaagta ttgaaaatcc tgaaatttat
     6721 taccagacaa atgtaatagg gaatttaaat cttcttgaat gtgcgaagga ctttaaaata
     6781 aaaaaattta ttcttgcctc aacatcttct ctttatgctg gttgtaatac accttttaaa
     6841 gaggacttgc ctgtaaataa tccaatttct ccttatgcta caagtaagaa agcagcagaa
     6901 cttacctgtt atacttacca ttatcttttc gggattgatg ttgttatttt cagatatttt
     6961 actgtttatg gaccatgtgg aagacctgat atgagtattt ttaagtttat aaaattgatt
     7021 gatgaggaaa aagagataac tgtttatgga gatgggacac agagcaggga ttttacctat
     7081 attgatgata ttgcagaagg gactatttca gggctcaatc tttctggttt ccatgtaata
     7141 aatcttggga acagcaatcc acatcaattg aatgaaatta taaaattaat tgaggaaaaa
     7201 ttgaataaaa aagcaaagat tatttatgct ccatttcata aagcggatat aaaggcaact
     7261 tatgctgata taacaaaggc aaaaaattta ttgaactgga ctcctaaaat tgatataaat
     7321 gaaggtattg aaagaacagt aaactggtat attgaaaaca gggattggct caaggatgtc
     7381 attttgaagg attaaaaatg tcaaaaattc ttgttacagg tggagcaggt tttataggaa
     7441 gtcatacagt tgatagattg gttaaagaaa ggaatgaagt tatagtaatt gataatcttt
     7501 caacaggttc actggaaaat ctgaatgaag aagttatttt ttataatttt gatataaggg
     7561 aaaaagaaaa gttaaaagag atttttgata attattattt tgattatatt tttcattttg
     7621 ctgcacagat aaatgttaga aagggagagg aagaccctat ctttgatgtt gatgtaaata
     7681 taggagggat tataaatatt cttgaatgtg caaaggaaaa accacccaaa aaaattattt
     7741 tttcttcaac aggtggagtt atgtatggat tgactgatat ttttccaagt cctgaaacta
     7801 ttgagccatt tcctatatgt gtttatggta tttcaaaatt aacaggtgaa aaactattat
     7861 atgcttactg gaaaaagtat ggaataaatt atgttgcttt aaggtatgga aatgtttatg
     7921 gtccgaggca gaattatctg tcagaagcag gtgttattgc aatttttata tcaagaatac
     7981 ttgatgggaa agaatgtata atttttggag atggaaacca gacgagggat tttattttta
     8041 ttgatgatgt tgttgaggca aatgttttat ttatgaagaa tgaaacaaca ggagttttta
     8101 atgtgggaac agggattgaa acaagtgtaa acagaatatt tgagattata aaagagaata
     8161 ttggaaaaaa ttgtaaaaaa gtttatggag aagaaagaaa gggagaatta aaaaggagtt
     8221 gcctatcaat tgaaaagatt aaaagtcaga ccaactgggc tcctaaatat aaaatagaag
     8281 agggaattaa aaaaacaatt gaatggtttc tctcaaaaag ataaattttt cagtaagatg
     8341 aggattccca ttcgggttgg cttataaaaa cattatagtc cctgaaagca agatttaata
     8401 ttttctgttt atgattgtag taatgccaga aacatcttac aattggtact tgtccaccat
     8461 aaccatgatt tgattgccag acctttgctt ctttccatga aactacacca tcgttgttta
     8521 catctgcctg ggcaatatct gtttggttat gagtactttc aaaccatttg caatgcgttg
     8581 gagcaaattc gtatgaatat gaacaggcct caacagatgg atttctatgg gcataagcat
     8641 tctggtcaaa ggtatcaggc gtatatgaag gataactatt ctgaactttt gataatggga
     8701 tatcacatat ttcaggaaag gatggatttc catgtccttt atttccttta ttcatatcgg
     8761 ttaaacagag aaaaatttta cctgttccga gataatttga accaattcca ggggtatcgc
     8821 ttttacagag taaagaaagc catgttggat attcatcata atcctgataa tacatttcaa
     8881 ttgcaagtcc aatctgatgg agattattaa tacagtttgc ctgtcttcct ttttctcttg
     8941 cagttgaaag tgatggtaaa atctggcccg caagcattga gattattgaa attacaacaa
     9001 gaagttcaat taatgtgaaa cctccttttt ttattctttt tgttttcatt ttttgcctct
     9061 cttttgaatt tctttaatta tctgataaac ataatttttc atttcatcat tctgctcatt
     9121 ataataaaga gcccataaag gtgtttctat ttcttctggt ttcccttttt cttttaatat
     9181 ttcaagtgct ttctttctga caggtatttc ttctttttca ttttcaacaa tttttaataa
     9241 tttttcctga tattccggag aattcatata atcaagtatt ttaaattttg tatccgtatc
     9301 ttccttatca aatctctcta taagataatc aatatttttc ttatcaattt tcaatatcgt
     9361 ttcaaatgtt atttccttta tttttttatc tgaactatca agatatgtaa gaagtttatt
     9421 aaaatcaatc tgtcctatac tccatgcaca aactctttta atttcctcat cgttttcttt
     9481 ttgaaataca tcctcaacat caggtaatgc ctcttttatc ttcagatttc cagcattata
     9541 aattgcatat attttttcat ttttgttcgg actttttatc tgttttataa atatttttgc
     9601 ggtaggagtt ttgtcttttt ttgcactcca gatagacact cttaaaattg taagaaaaat
     9661 aacacctaca caaacccaaa atattattct attctgttta ttcaatcctt tataaaactc
     9721 aataactttt ttcattttag taaataatac cccccttttt tatttttgtc aatattttct
     9781 ttcaggaaat tcttattatg cattcctttc tgaattttga aaaattaatt ttttctataa
     9841 atttttttgt tttcctatcc ctgtatcctg aaatatcctc atccatattc aaacttttta
     9901 ttatactctt cataattatt tctctcatat atttacctat tattgaagaa aaggaagcaa
     9961 ctggtgatat gttttcaaca ttcttataaa atccaagttc aaaagaaaga tttttttttg
    10021 aaattttata ttcagaaaag tcgtcagttt cctttaaagc ataaatttca taatcaggta
    10081 aaaaatattt tagaaaattt ctgtaataga tggtaccccc aatttttcca cagaagaatt
    10141 ttatattttt gtagttttcc atctctttta ttatttcaca gaaattgaat aaatttaaaa
    10201 gaaattttga tttttttttc tttacaaaat cgttaaaaaa atacgggcat acagttttta
    10261 cctctattct tttaatttta tctttttctt tcagaagatc actgtatctt tttataattt
    10321 cttttatgtt ttcactttca aaaaaaaggg ggatattttt ttcacacagg ttttcttcaa
    10381 aggcacattt tttataactg aatcttttaa atatttcgta tggagaggaa ggtgtcctat
    10441 tttcaaccat ataaaaagaa ataaccgcaa tttcttcaat tttttttaag tcctttttat
    10501 tcttataaaa aattttgcta tcctttatat aatcaggaat ttttatatct tcagaagtta
    10561 agataccggt aattacaagc ggacctaaaa caggaccaaa accattttca tctattccac
    10621 atatatattt ttccattatt ttcattttat cattttaaaa aacaattcaa aacttgatgg
    10681 taaagataat aaaaagtaaa attaaattat gaggttcaaa aggcactgtg agatagtaaa
    10741 aggggagata aatcttactc ctatggtaga tgttgttttc cagttgatta tattttttat
    10801 gttaagttca agttttatta tgcagcccgg aataaaaata aagttaccaa aagcagaaac
    10861 tacagacata ataaaacaga gagagatttt tgttaatatt acagaagaag ggaaaattta
    10921 ttttaaaggg aaggttgtta atctttcaga atttgaaaaa gaattaaaag aggaattaaa
    10981 gaatagaaaa gaaaatataa tacttattgt taaagcagat aaggatacaa aacacggaat
    11041 agttgttagt gttatggata aggcaagaag ttgtggcatt gaaaaaatcg cagttgcaac
    11101 aatgccagaa ttttaaaatg agaacaaatt ccatttttct taaagcaatt tttataagtt
    11161 tttctattca tattcttggg ctatctcttt tttctattgt actgccaggc cctctaaaaa
    11221 aaataaaacc aatagaagtt gttatttatc cttctctgga aacaagacaa atcagggaaa
    11281 atattaattt tgtttcaaaa aagatagagg agaacat
//
LOCUS       DAOSAT010000061        11184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3875_length_11184_cov_4.667535, whole genome shotgun sequence.
ACCESSION   DAOSAT010000061 DAOSAT010000000
VERSION     DAOSAT010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3875_length_11184_cov_4.667535"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4519)
                     /locus_tag="PLF90_06315"
     CDS             complement(<1..4519)
                     /locus_tag="PLF90_06315"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOK56925.1"
                     /translation="MKKLFIFILLSSTLLFGDTIKGIVVDLKDNPVKNAEIKVEAIAP
                     LTEKDTKTAKTDKKGEFIIENLKTGYYYLRCNASEFLTCNMGRIEVNQAKYGIKSYKI
                     VMQKPGSISGYVYDENSKPIERAKVGNYSVSVFTDKNGFYRITNLYPGKIYLYCEKEE
                     YIRAEKQNIIVEENKETGGINFTLQIGGSISGEILDDENKSIKNSSVYTEGNMYKWTR
                     TDEKGHFIIKGLLPGIYKISVYAQGYEYGYAGNIEVKKGETTKIAPVHLKLRPKNFYL
                     FTRTSTFLPTENVTLFYNSFRITKVKVDVYKVDIFSEINKIRWREAPNLYNLQNNIKT
                     DGYEPLFSKEFKIEYPSPLSDLYHKTIEISKLPAGVYIIVAKPEDFSEQRKLIIVTDI
                     GIICKSAEKQSYLYLVDLITGKKLPEVKVYFSYYDSKLGEYKFKTKTTDENGIIEFEN
                     FSGTLFASKENSFAYIDYGYERSFPKEKIYVYTDRPVYRPEQTVYFKGILRKDEGDHY
                     LLKNINSVIVNITDPMGNNVYSTNAEVKNGSFSGSFTIPDEAPLSLYYLNVYNPEYDY
                     QGSVSFKVLEYRKPEFFVEVKSDKERYLPNEKVKILINSKYYFGAPVKNADVSYAVYE
                     TPLLEYDDYYEGKYEGEYYGYRKYLTSGETKTDEKGEVLIEFMTKSSYENETIYTVEV
                     RVTDISKREVKSDCSFKVVPGNFRINITTTKYLYTHSENIPVKIEVMNYDGIPVKNQK
                     INLKVDLEKYKNKKFYYKTILADTIKTDEKGKIEINIKPDVCGYIRISASSIDEFNNL
                     ITSNKFIWIASENYYFNYGKKELEIITDKKNYRTGENAKILINSSIPDLTLFLTIESY
                     RIHETKIFEMKGNSVLIEIPIKKEYLPNVYLSVFSVRNKKFYEITKNIKIAPDEKFLK
                     VDIITDKDNYTPGEKAIYRIKTTDFKGNPVSAELSFQVVDEAIYAISPELQSKIEDFF
                     YGNKPNFVSTTYSFIKHSYGGTSKDIKDIDIRRKFKDTAYWNPYIFTDRNGNATVEVE
                     LPDNLTTWRAKAVAVTHDTLVGTGINKIKTSKPLIARLILPRFLVEDDILFVSGIIHN
                     YTKKAQDLKVVLKGDGFEMLDKNEVIISVEPDSEKRVQWQIKVKDIDKAKFTLIAWNS
                     EVNDGMELEIPVYLYGNEIREVKSGKCDKEITEKFDIPVNSYKVNLISIIYPSIASGM
                     YHALEYLVNYPYGCTEQTMNTFLPAIYVNLALKNLGVEDLSFLADNIFYFQNMLQKLP
                     KIMNKGLSKLYRYQHDDGGWGWWESDPSHPYLSSYVMYGLSQIKKVGYIVNENVYSKG
                     KNYLKNILPDIKENDTLIYVLYSLFESCSENMTDGEDKIIREYSEKLYNNLISQKTIH
                     PYTGAQLSLILNKFDKNKAKAILDKIYKDIKLLTPYSAFFICEKENSYRWIDNNIEGT
                     AWVLKATLQIEPEREEIYKIIRYLVETRRLGYWRSTKETAVCINALTDFLLKNPSELH
                     PDYSFLLYLNNE"
     gene            complement(4534..5154)
                     /locus_tag="PLF90_06320"
     CDS             complement(4534..5154)
                     /locus_tag="PLF90_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HOK56926.1"
                     /translation="MFLVFRKIFKNFGKIITILIFLFISITNSQIDLKLEALKLSRET
                     LITYLKTNRIPEVNSILNSIPSKSIFITLTKNGKTRGCAGSFIPIYNSLGESVINFTI
                     IAATQDIRYNPVKIEEIDEIIITITFPERTVCIENPYMIKPLKEGLIIRKDGKEGVVL
                     PGEAKTVEYAIKIGLRNANLNNLNGAIFYKFNGSSFSEKDLKYEKN"
     gene            complement(5157..6602)
                     /locus_tag="PLF90_06325"
     CDS             complement(5157..6602)
                     /locus_tag="PLF90_06325"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011249313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOK56927.1"
                     /translation="MAEELKIEKINEYKWMIPKVGEMRVPGIIFISEKLLKKAIEDKA
                     IEQVANVAKLPGIVKYSLAMPDVHWGYGAPIGGVGAFNEETGVIVPGFVGYDINCGVR
                     LIRTDLTRKDIENKLKELVDGLFYNVPSGVGSTGKLRLKRKELEELVVKGAKWAIEKG
                     FGKREDLENIEEYGCLEGANPDVISDRAYERGLEQSGTLGSGNHFLEIQEVIHIYDGK
                     IAEKFGLFNGQITIMVHTGSRGFGYQICDDYLDMFGKVVHKYGIKLPDRQLACAPASS
                     PEGQKYFKAMKAAANYAFANRQMIYYWICETFEKILKIPAEKLYIETVYDVAHNICKF
                     ETHTVNGNKKRVYVHRKGATRAFPKNHPEIPEKYKDVGQPVIIPGTMGTASYVLIGTE
                     KAMEETFGSTCHGAGRMMSRSQAIKEAKGRSISKELLEKGIIPKAVSEKGLAEEMPGA
                     YKDVDEVIKVVEGVGISKKVAKMIPLAVIKG"
     gene            6709..7242
                     /locus_tag="PLF90_06330"
     CDS             6709..7242
                     /locus_tag="PLF90_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOK56928.1"
                     /translation="MEEITKKILNENGIENFEELVKKYEKMVYGIAYRSLNNKQDAED
                     ITQETFLIIYKKIKDLKKYNSLTNWIYTIALNLTREYMRKNYSKKNLYHILESTLNIK
                     NENNDSNYLKEILKEKIKSSLEKLSSQQKEAIELKYLKDLKIKEISKIMNCKEGTVKV
                     HLFRGIRALKEVLKNEK"
     gene            7232..7660
                     /locus_tag="PLF90_06335"
     CDS             7232..7660
                     /locus_tag="PLF90_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOK56929.1"
                     /translation="MKSSENLEENLILYIEGKLNDKEKKEIEEHIKICERCRSYVEYV
                     KKLLPSSEENTEDNYWIILKEKILKRIEDYKETRFSRIIRIKKITLSFMIVFILATTF
                     FNIFQEQINLIRHLHLFKDYHIIKNLDTLKEIIAMEEENE"
     gene            7653..8006
                     /locus_tag="PLF90_06340"
     CDS             7653..8006
                     /locus_tag="PLF90_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56930.1"
                     /translation="MNKRDRILNVFLVITTFLFIFTAYSYFYKKEHPIKSYLYPEELK
                     TIYEMENFLHNYKIYFSISEKEKNKIWENFYNFLQLDEEEKSSLLQSYVEFLKYDKEK
                     RETLRKIYNEIFENM"
     gene            8090..8524
                     /locus_tag="PLF90_06345"
     CDS             8090..8524
                     /locus_tag="PLF90_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56931.1"
                     /translation="MKKIITGLTCIMFITSFGISLFAAEKSTTEKPKSSATPAQPATP
                     ATPATPAQPGAPASEAKVKIQKVVGEITNIDTTANTIKIKKEDGTEITLKATTPKMQE
                     EINKLATGKKVTALYKETKTGEYTLIKISEYKEKPTTEKPKK"
     gene            complement(8548..9135)
                     /locus_tag="PLF90_06350"
     CDS             complement(8548..9135)
                     /locus_tag="PLF90_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOK56932.1"
                     /translation="METINKVRIILGGSGGQGILTLGKLLAYAGIKKNYEVSCLPTYG
                     AEMRGGYIYCFVIISKEKDIFSPLTESCDIGVFLNEMSYKMLKKFLKKDAYSILNYSL
                     IKNFKKNEKVIEIPATEIAEKTGDIRITNMVMAGVVSKLISQKFFDYEKEILINEINK
                     IMEKEEIIKLCEKGIKEGWQYLTFKEKNKKDCHQI"
     gene            complement(9119..10072)
                     /locus_tag="PLF90_06355"
     CDS             complement(9119..10072)
                     /locus_tag="PLF90_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK56933.1"
                     /translation="MTPKERFINALERKPIKGRVPHFELVFFLTMEAFGKVHPSHRNY
                     SQWYQMTEKERNLHRKDMAEIFIKTAETFEHDAIFLHPNPPTLEETIRLIDLVREMTG
                     NKYFLMIHGDATFGVPSGDKMMEFIERIADEPKKLKKEADDMVNEALKKAEIIKEKTY
                     LDGFALCSDYCFNSGPFLSPNMFSEFVTPYLAKLIKGYRDMGFYTIKHTDGNIMPIID
                     QLVETNPHALHSLDPQAGVDIKVVKEKYGNRICLIGNVNCGLLQTGTDQEVIESCLYA
                     LKYGMPGYGYIFSTSNCIYTGMPLERYLLMLGIWKEYGNYK"
     gene            complement(10077..>11184)
                     /locus_tag="PLF90_06360"
     CDS             complement(10077..>11184)
                     /locus_tag="PLF90_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56934.1"
                     /translation="FLMPKDVLLSVWLFAFLFVIETGFFKMTGYSMGQILPYSDPACQ
                     EVAFQSFGALIVLSLSCLWVARKHLKEIFLKSIGKLKDEDEGISSKITFWGFVFCFIF
                     VIWWINRTGLKLLPSIYFTIITILIFLGMTRIISQAGLAYYRAPLIPSAATIYTFGSQ
                     TLGNNGLVSLGLTFPWAADIRTLVMVSTANGLKLADNFKLNLKKLVIAILLSIIFTLL
                     SSSIMTLFLAYKYGGINLSSWQFGGATNFMANYIMNIIKEPITFGKKQFLFSGIGAFL
                     MILILFARSRIPGWPLSPIGLAIGIPHPVFHTWFSVFIAWIAKTLIMKYGGAKVYSEG
                     KIFFLGMVCGSFVTAGLWIFVSWMTKTSIGFTLG"
BASE COUNT         3777 a         1835 c         1395 g         4177 t
ORIGIN      
        1 tttcattgtt aagatataag aggaaagagt aatctgggtg aagttctgaa ggatttttca
       61 aaaggaaatc tgtcaaagca tttatacaaa ctgctgtttc tttagttgac ctccagtaac
      121 ccaatcttct tgtttcaaca agatatctta ttattttata aatttcttct ctttcaggtt
      181 caatttgaag tgttgctttt aaaacccatg cagttccttc aatgttgtta tcaatccacc
      241 tgtaagaatt ttctttttca cagatgaaaa atgcagaata aggagttaaa agttttatat
      301 ctttataaat cttatcaaga attgcttttg ctttgttttt atcaaattta ttcagaataa
      361 gagaaagttg tgctcctgta tatggatgga ttgttttttg tgaaattaaa ttattgtaaa
      421 gtttttcaga gtattccctt attattttat cttctccatc tgtcatattt tctgaacaac
      481 tctcaaataa tgaatatagg acataaatga gagtgtcatt ttctttaatg tccggtaata
      541 tatttttcag ataattttta ccttttgaat atacattttc attaacaata tatcctactt
      601 tttttatctg tgagagtcca tacataacat atgaacttaa ataaggatga gatgggtcac
      661 tttcccacca tccccagcca ccatcgtcat gctgatatct gtataatttt gaaagtcctt
      721 tattcataat ttttggtaat ttctgaagca tattttgaaa atagaaaata ttgtcagcaa
      781 gaaaagataa atcctcaact ccaagatttt ttaaagcaag atttacataa attgcaggta
      841 aaaatgtgtt cattgtctgt tctgtacaac catatggata attaacaaga tattcaagtg
      901 catgatacat acctgatgca attgagggat atattattga aatcaaattt actttatatg
      961 agtttaccgg tatatcaaat ttttctgtta tttccttatc acatttcccg cttttaacct
     1021 ctcttatttc atttccgtat agataaacag gtatttcaag ttccattcca tcatttactt
     1081 ctgaattcca tgcaataagt gtaaactttg ctttatctat atctttaact tttatctgcc
     1141 actgaactct tttttcacta tcaggttcaa cagaaataat aacttcattt ttatcaagca
     1201 tttcaaatcc atctcctttc aaaacaactt ttaaatcctg tgcttttttt gtatagttat
     1261 gaataattcc tgaaacaaaa agaatatcgt cttcaacgag aaatctcggt aatattaatc
     1321 ttgcaataag tggtttggat gtttttattt tatttattcc tgttccaaca agtgtatcat
     1381 gtgttacagc aactgctttt gctctccatg ttgtaagatt atcaggaagt tctacctcaa
     1441 cagttgcatt tccatttcta tcagtaaata tgtatggatt ccagtaagcg gtatccttaa
     1501 attttcttct tatgtcaata tccttaatat cttttgaagt accaccgtat gaatgtttaa
     1561 taaaagagta agttgttgaa acaaaatttg gtttgttacc atagaaaaag tcctcaattt
     1621 tgctctgtaa ttcaggtgat attgcgtaaa ttgcttcatc cacaacctga aaggataatt
     1681 cagcagaaac tggatttcct ttaaaatctg ttgtttttat tctgtaaatt gctttttcac
     1741 caggagtata attatcttta tccgtaatta tatcaacctt taaaaatttc tcatcaggtg
     1801 caatttttat attttttgta atttcataaa actttttatt tctaacactg aaaactgaaa
     1861 gataaacatt tggcagatat tcttttttta ttggtatttc tattaaaaca gaatttcctt
     1921 tcatctcaaa aatttttgtc tcatgaattc tgtaactttc aatagtcaag aataatgtta
     1981 aatcaggaat tgaagagttt atcagtattt ttgcattttc tccagttctg taattttttt
     2041 tatcagttat tatttcaagt tcttttttgc catagttaaa gtaatagttc tcagaagcaa
     2101 tccagatgaa tttatttgat gtaataaggt tattgaattc gtcaatagaa gaagcagaaa
     2161 ttcttatata tccgcataca tcaggtttta tatttatttc aattttacct ttttcgtctg
     2221 tttttatagt atctgcaagg attgttttat aataaaactt tttgttctta tatttttcaa
     2281 ggtcaacttt caaatttatt ttttgatttt ttacaggaat tccatcataa ttcataactt
     2341 ctatttttac aggtatattt tctgaatgtg tataaagata ttttgtagtt gttatattta
     2401 ttctaaaatt tccaggtaca actttaaagg aacaatctga tttaacttct cttttactta
     2461 tgtctgttac tctaacttca acagtatata ttgtttcatt ttcatatgaa ctcttcgtca
     2521 taaattcaat aagtacttct cccttttcgt cagtttttgt ttcaccagaa gtaagatatt
     2581 ttctgtaacc atagtattca ccttcatatt ttccttcata ataatcatca tattccagta
     2641 aaggtgtttc atatactgca taacttacat ctgcattttt tacaggtgct ccaaaataat
     2701 attttgaatt tataagtatc tttacttttt cattgggcag atatctttcc ttatcacttt
     2761 taacttctac aaaaaattcc ggttttctgt attcaagtac tttgaaggag acacttccct
     2821 gataatcata ttcaggattg taaacattta aataatataa actaagaggt gcctcatcgg
     2881 gaattgtaaa tgaaccggaa aaagaaccat ttttaacttc tgcatttgtt gaatagacat
     2941 tatttcccat cgggtcagtt atattaacta ttacagaatt aatatttttc agtaaataat
     3001 ggtctccctc atcttttctt aaaattcctt taaaataaac tgtctgttca ggtctgtata
     3061 ccggtctatc tgtataaaca taaatttttt ctttaggaaa tgacctttca tatccataat
     3121 ctatataagc aaaactgttt tcttttgatg caaaaagagt tcctgaaaaa ttttcaaatt
     3181 caattattcc attttcatct gttgtttttg ttttaaattt atattctcca agtttactgt
     3241 cataataaga aaaataaact ttaacttccg gtaatttttt ccctgtaatt aaatcaacta
     3301 aatatagata actttgcttc tctgcacttt tacagataat tccaatatca gtaacaatta
     3361 ttaactttct ctgttctgaa aaatcttctg gcttggcaac tattatgtaa acacctgctg
     3421 gcaatttact tatttcaatt gttttatggt aaaggtcact taatggtgaa ggatactcaa
     3481 ttttaaactc ttttgaaaat aaaggttcat aaccatctgt ctttatgtta ttttgaagat
     3541 tgtaaagatt tggtgcttct ctccatctga ttttgtttat ttcagaaaaa atgtcaactt
     3601 tataaacatc aacttttacc ttggtaattc taaatgaatt gtaaaataaa gttacatttt
     3661 cagtaggcag aaaagtactt gttcttgtaa ataaataaaa gttttttggt cttaatttta
     3721 aatgaacagg tgcaatttta gtagtttctc cttttttaac ttcaatatta ccagcatatc
     3781 catattcata tccctgagca taaactgaaa ttttatatat accgggaaga agacctttaa
     3841 taataaagtg tcctttttca tctgttctag tccacttata catattacct tcagtataaa
     3901 cactggaatt ttttatggat ttattttcat catctaaaat ttcacccgaa atacttcctc
     3961 ctatttgcaa agtaaaatta attcctccag tttccttatt ttcttccaca attatatttt
     4021 gtttttctgc cctgatatat tcttcttttt cacagtaaag atatattttt cctggataaa
     4081 gatttgtaat tctataaaat ccatttttat ctgtaaaaac actgactgaa taattaccaa
     4141 cttttgctct ttctattggt tttgaatttt catcgtaaac ataaccactt attgaacctg
     4201 gtttttgcat aacaatttta taactcttaa ttccatattt tgcctgattt acttcaattc
     4261 ttcccatatt acaggttaaa aattcggaag cattgcatct taaatagtaa taacccgtct
     4321 ttaaattttc aattataaat tctccttttt tgtctgtttt tgctgttttt gtatcttttt
     4381 ctgtaagagg tgctattgct tcaactttaa tttcagcatt tttcacagga ttatctttta
     4441 aatcaacaac aattccttta atagtatctc caaaaagaag tgtactgctc aataagataa
     4501 aaataaacaa ttttttcatt tcttacccca tttttagttt ttttcatact ttaaatcttt
     4561 ttcagaaaaa ctgcttccat taaatttata aaaaattgct ccatttaaat tgtttaaatt
     4621 tgcatttctt aaacctattt ttattgcata ctcaactgtt tttgcttctc ctggtaatac
     4681 aaccccttct ttaccatctt ttctgattat aagcccctct ttcaatggtt ttatcatata
     4741 tggattttct atacaaactg ttctttctgg aaaagttatt gttataataa tttcatcaat
     4801 ttcttctatt tttacaggat tgtatcttat atcctgtgtt gcagcaatta tggtaaaatt
     4861 aataacactc tctccaaggg aattgtaaat cgggatgaaa cttccagcac atcctcttgt
     4921 ttttccgttt ttagtcaatg ttatgaagat tgattttgaa ggtattgaat ttaatataga
     4981 attgacttcg ggtattcggt ttgtttttaa ataagttatt aaggtttctc ttgatagttt
     5041 gagtgcttca agttttaggt caatttgaga atttgtgata gaaataaata gaaaaattaa
     5101 aattgtaata attttaccga aattcttaaa tatttttctg aaaactaaaa acattcttat
     5161 ccttttataa cagcaagcgg aatcattttt gcaacttttt ttgaaattcc aacaccttca
     5221 acaactttta tcacctcatc aacatcctta taagcacccg gcatttcttc tgcaagtcct
     5281 ttttcgctca ctgcttttgg aataatacct ttttccaata attcctttga tatacttctt
     5341 ccttttgctt ccttaattgc ttgacttctt gacatcattc ttcccgctcc atgacaggta
     5401 cttccaaatg tctcctccat tgctttttca gttccaataa gaacataaga agcggttccc
     5461 attgtccccg gaattataac aggttgacca acatctttat atttttcagg aatttctgga
     5521 tggttttttg gaaatgccct tgttgctcct tttctgtgaa cataaactct ctttttattt
     5581 ccgtttacag tatgcgtttc aaatttacag atattatgtg caacatcata aactgtttca
     5641 atatataatt tttctgcagg gatttttaaa attttttcaa atgtctcgca tatccagtaa
     5701 taaatcatct gtctgtttgc aaaagcataa tttgcggctg ctttcattgc cttaaagtat
     5761 ttctgcccct ccggagaact tgctggagca caggcaagtt gtctatctgg taatttaatt
     5821 ccatatttat gaacaacttt tccaaacata tcaagataat cgtcacatat ctgatagcca
     5881 aatccacgac ttccagtatg aaccattatt gtaatctgtc cattaaaaag accgaatttc
     5941 tctgctatct taccatcata aatatgtata acttcctgaa tctcaagaaa atggtttcct
     6001 gaaccaaggg ttcctgattg ttcaagccct ctttcatatg ctctatcact tataacatca
     6061 gggtttgctc cttcaagaca gccatattct tctatatttt caaggtcttc ccttttacca
     6121 aatccttttt caatagccca ttttgctcct tttacaacga gttcttcaag ttcttttctt
     6181 ttcaatctca attttcctgt agaaccaaca ccggaaggga cattataaaa taatccatca
     6241 accagttctt taagtttatt ctctatatct tttcttgtta aatctgttct tattaatcta
     6301 actccgcagt ttatatcgta gccgacgaat ccagggacta ttacacctgt ctcttcatta
     6361 aaagcaccaa ctccaccaat tggagcacca taaccccagt gaacatcagg cattgccaaa
     6421 ctatatttaa ctatacctgg aagttttgcc acatttgcaa cctgttcaat tgctttatct
     6481 tcaattgctt ttttaagtaa tttttcagaa ataaaaataa taccgggaac acgcatttca
     6541 cccactttgg gtatcatcca cttatattca ttaatctttt ctatttttaa ctcttcagcc
     6601 atttttgact cctttatcat tttctaatat tataccttaa ataaataagt taaatgaaat
     6661 aatgtaaact ttttgttaaa aaataaactc tattataata aaacttccat ggaagagatt
     6721 acaaaaaaaa ttttaaacga aaatgggatt gagaattttg aggaacttgt aaaaaaatat
     6781 gaaaagatgg tttatggaat tgcatatagg tctttaaata ataaacagga tgcagaagat
     6841 ataacacagg aaacattttt gattatttac aaaaaaataa aagatttaaa aaaatacaat
     6901 tcacttacaa actggatata tactattgcc ttaaatttaa cgagagaata tatgaggaaa
     6961 aactatagta agaaaaatct ttatcatatt cttgaaagta ctttaaacat aaaaaatgag
     7021 aataatgact caaattactt gaaagaaatc cttaaagaaa aaataaaatc ttcacttgaa
     7081 aaactttcat cccagcaaaa agaagcaatt gaacttaaat atttgaaaga tttaaaaata
     7141 aaagaaattt caaaaatcat gaattgcaaa gaagggacag tgaaagtaca tctttttaga
     7201 ggaattaggg cattaaagga ggttttaaag aatgaaaagt agtgaaaatc ttgaagaaaa
     7261 cctaattctt tatattgaag ggaaactgaa tgataaagaa aaaaaagaaa tagaagagca
     7321 tattaaaatc tgtgaaagat gcaggtctta tgttgaatat gtaaaaaaac ttctaccttc
     7381 ttctgaggaa aatacagaag acaattactg gattatcttg aaagaaaaaa ttttgaaaag
     7441 aattgaagat tataaagaaa ctcgtttttc aagaataatt agaataaaaa aaattactct
     7501 ttcttttatg attgtattta ttttagcaac tacttttttt aatatttttc aggaacagat
     7561 taatcttata agacatctac accttttcaa agattatcat attataaaga atcttgatac
     7621 tttgaaagaa ataattgcaa tggaggagga aaatgaataa aagagataga attttaaatg
     7681 tttttttagt aataactact tttctattta tttttactgc ttactcatat ttttataaaa
     7741 aagaacatcc aattaaaagt tatctctatc cagaggagtt aaagacaata tacgaaatgg
     7801 aaaatttttt gcataattat aagatttatt tttcaatttc tgaaaaagaa aaaaataaaa
     7861 tatgggagaa tttttacaac tttttacaac tggatgaaga agaaaaaagt tcattacttc
     7921 agagttatgt tgaattttta aaatatgata aagaaaaaag agaaactctg agaaagatat
     7981 ataatgaaat ttttgagaac atgtaaactt ttttaaattt acaattatct ttatattaaa
     8041 ggactgccac cagaacaaaa taaataaaaa agaaaggagg tgaaaaaaca tgaaaaaaat
     8101 tataacaggt ttaacctgta taatgtttat aacgtctttc ggaatttcac tttttgccgc
     8161 tgaaaaaagt acaactgaaa aaccaaaatc atcagcaaca cctgcacaac cagcaactcc
     8221 tgccactcca gcaactcctg cacaaccagg agcccctgcg agtgaagcaa aagtaaagat
     8281 tcagaaagtt gtaggtgaaa ttacaaatat agatacaaca gcaaatacaa ttaagattaa
     8341 aaaagaagat ggtacagaaa ttactttgaa agccacaact ccaaagatgc aggaagaaat
     8401 aaataaactt gcaaccggta aaaaagtaac tgctctttat aaagagacaa aaactggaga
     8461 atatacttta attaaaattt ctgagtataa agaaaaacct acaacagaaa aacccaaaaa
     8521 ataagtatct ttaattttct gtattaatta aatctggtgg cagtcctttt tatttttttc
     8581 tttaaaagtt aaatactgcc aaccttcctt tatacccttt tcacataact ttataatttc
     8641 ttccttctcc attattttat ttatttcgtt tattaatatt tctttttcat aatcaaaaaa
     8701 tttttgtgaa attaatttag aaacaacacc tgccataacc atatttgtaa ttctaatatc
     8761 tcctgttttt tcagcaattt cagttgcggg tatttcaatt actttttcgt tttttttaaa
     8821 attttttata agagaataat ttaatatgga ataagcatct tttttcagaa attttttaag
     8881 cattttataa gacatctcat ttaaaaaaac acctatatca caactttctg ttaatggaga
     8941 aaaaatatct ttttcttttg aaattattac aaaacaatat atatatccac ctctcatttc
     9001 tgcaccatat gttggtagac aactcacttc ataatttttt ttaatacctg cataagcaag
     9061 taattttcct aatgttaaaa ttccctgtcc acctgaacca cctaaaatta ttctaacttt
     9121 atttatagtt tccatattct ttccaaatac caagcattaa aaggtatctt tcaagtggca
     9181 ttcctgtata tatacagttt gatgttgaaa aaatatatcc atatcctggc attccgtatt
     9241 taagagcata taaacaagat tcaattactt cctggtctgt tcctgtttga agtaatccac
     9301 aattgacatt tccaataagg caaattctat taccatattt ttcttttaca acttttatat
     9361 caactcctgc ctgagggtca agtgaatgta aagcatgagg atttgtttct acaagttggt
     9421 caattatagg cattatatta ccatctgtat gtttaattgt ataaaatccc atatctctat
     9481 atccctttat taattttgca aggtatggag taacaaattc agaaaacata ttaggactta
     9541 aaaatggacc tgaattgaaa caataatcac tacataaagc aaatccatca agatatgttt
     9601 tttcttttat tatttctgct tttttcaatg cttcatttac catatcatct gcttcttttt
     9661 ttaacttttt tggctcatct gctattcttt caataaattc catcatttta tctccggaag
     9721 gaaccccaaa agttgcatct ccatgtatca tcaaaaaata tttatttcca gtcatttctc
     9781 taactaaatc aattaatctt attgtttctt ccaaagtcgg tggatttgga tgaagaaaaa
     9841 tagcatcatg ttcaaatgtt tctgctgttt ttataaatat ttctgccata tcttttctat
     9901 gtaaatttct ttccttttct gtcatctgat accattgaga ataatttctg tgtgaaggat
     9961 gaacttttcc aaatgcttcc atagttaaga aaaatacaag ttcaaaatga ggaacccttc
    10021 ctttaatagg tttcctttca agcgcattta taaacctttc ttttggtgtc atttttttat
    10081 ccgagagtaa atccaataga tgtttttgtc atccaactta cgaaaatcca caaacctgct
    10141 gtaacaaaac taccacatac cataccgagg aagaaaattt taccttctga ataaactttt
    10201 gctcctccat atttcataat taaagttttt gctatccagg caataaaaac agaaaaccag
    10261 gtatggaaaa caggatgggg tataccaata gcaagtccaa taggtgataa tggccaacca
    10321 ggaattctac tccttgcaaa taaaattaaa atcatcaaaa atgctcctat tcctgaaaaa
    10381 aggaattgtt tttttccaaa agtaattggt tctttaataa tgttcataat ataatttgcc
    10441 ataaaatttg ttgctccccc aaattgccat gaagataaat taattcctcc atacttataa
    10501 gcaagaaaaa gtgtcataat tgaagaactg agtaaagtaa aaattattga aagtaaaatt
    10561 gcaattacaa gtttttttaa gttaagttta aaattgtctg caagtttcag tccatttgct
    10621 gttgaaacca ttacaagagt tctaatatca gcagcccagg gaaatgttaa tccaagagaa
    10681 accagtccat tattacccag tgtctgagaa ccaaaagtat aaattgttgc agcagaaggt
    10741 ataagaggag ctctgtaata tgcaagtcct gcttgagaaa ttattcttgt cattccaagg
    10801 aaaattaaga tagtaataat ggtaaaataa atagatggta gtagtttaag acctgttcta
    10861 ttaatccacc agatgacaaa tataaaacag aaaacaaaac cccagaatgt aattttactg
    10921 gaaatccctt catcttcatc ttttaatttt ccaatgcttt ttaaaaatat ctcttttaaa
    10981 tgttttctcg caacccacag acaacttaaa gaaagaacaa ttaaagcacc aaatgattga
    11041 aatgcaacct cttgacatgc agggtctgaa taaggtaaaa tctgtcccat tgaatatcct
    11101 gtcatcttga aaaaaccagt ttcaataaca aaaagaaagg caaagagcca cacacttaaa
    11161 agaacatctt ttggcattaa aaag
//
LOCUS       DAOSAT010000062        11092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3919_length_11092_cov_4.899610, whole genome shotgun sequence.
ACCESSION   DAOSAT010000062 DAOSAT010000000
VERSION     DAOSAT010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11092
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3919_length_11092_cov_4.899610"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PLF90_06365"
     CDS             complement(<1..544)
                     /locus_tag="PLF90_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOK56935.1"
                     /translation="MGWIILAVFIFMILANAIKIVNEYERGVVFRLGRLVGARGPGLI
                     FLIPIIEKMVKVSLRTVTFDVTPQEVMTKDNVPIKINAVVWFRVMDPVRSVVEVENYQ
                     LSTMQIAQTTLRGVAGQLELDEILAEREKINHRLQQIIDEQTDPWGIKVSIVEIKEVE
                     LPDTMKRAMARQAEVERERRA"
     gene            654..2414
                     /locus_tag="PLF90_06370"
     CDS             654..2414
                     /locus_tag="PLF90_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK56936.1"
                     /translation="MLQNNNFKNGKFDIYFKYLSDYKKVAILAPLFMILEVIMDLLQP
                     KLLSTIIDDGIVKNNLKLIINTGFFMFFIAIIGLIGGIGCTIFSSIASQNFGHDLRKD
                     IFKKIQNAKFKEITKFSPSSLITRLTNDIAQSQQLVLISLRMLVRAPLLCIGGIIMAF
                     SINFKLSLIIFIVMPLLLSIFYFISKKSFILFTDMQKKIDRLNLVIRENLAGIRVIKI
                     FNRFAYEKKRFEKSNEDLMEASLKAINLIIKMGPLVMIIVNLSVITVLWFGGKMVLTN
                     EIKVGEIMAFINYLMIILMSLMMVSNLFIFISRAGASIERINEVLKIEGVKVYEKYSP
                     LDMIKGKIEFKNVFFSYDDNSDYYFLKDISFKIEEGETVGIIGTTGSGKTTILNLIAG
                     LYEPISGEILIDDVNIKNINPDNLKKNIGFVTQEIVIFSGTIREIMKWASEETSDEEI
                     IEALKIAQIYDFVSNLPEGLDTFIGQKGVNLSGGQKQRLTIARAIIKKPKILILDDCT
                     SSVDYITEKKILKNLKEKLNNSTKIIVTPRIFTIAEADRILVIDKGEIVGEGRHTELL
                     EKCEFYREIYETQIGEKING"
     gene            2407..4260
                     /locus_tag="PLF90_06375"
     CDS             2407..4260
                     /locus_tag="PLF90_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK56937.1"
                     /translation="MVEKDKFYEQQKIKYVRGHFFGPPGPGRHMNLLSEKPKETIKTL
                     KKLFKYLGKYKLYFLLSFVCILGSSILFLIGPYLIGKGIDYYIIPKNLSGFGKFLLFM
                     LVIYIFNSLLVYIQSLTMANISQRVGYDLRKDAFECIHKLFLKVIDKRTIGDIMSRIT
                     NDIDMINMTLATSITQFFSGIIMLSGTIVMMIILSPLLTLFSIILIPVMIFITKIISG
                     KTRKYFFIQQTKLGELNGIAEEDITGLRIIKIFGKEEKEIEKFEKINEELKEYGINAQ
                     KFAGIMPPLMNFLNNISFAIIGGIGGVFVLKKIITIGTIASFINYVKQFTRPLNELAN
                     QFNTIQTAIASAERVFEIMEEEKEKEDEEGAIELKEIKGEVEFKNVWFAYEDEKYVLK
                     NINFKVPAGKIIAIVGPTGAGKTTIINLLARLYEVEKGEILIDGYDIRKIKKESLRSF
                     LGVVLQDTYLFAETVKENIRYGKLEARDEEIQEVCRVSNAEKFILNLPKKYQTVLSDS
                     GQSLSQGQRQLIAISRAILAKPKILILDEATSNIDTKTEMDVQKSMLNLMKDKTNFVI
                     AHRLNTVKNADLILVIKEGEIIEKGNHKELMNKKGFYYNLFTSQFGEVLND"
     gene            4387..5154
                     /locus_tag="PLF90_06380"
     CDS             4387..5154
                     /locus_tag="PLF90_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOK56938.1"
                     /translation="MKYKLMEMTLKEVKEFGKVEVCVLPWGSCEPHNFHLPYGCDTFT
                     VEKIAEISCKKANEKGGKVILLPTISIGVNSNHFGFPMVLHLSPTTQLSILKDIVKTL
                     ENYKIHKFVLINGHGGNEFKGLLRELYGQTKVFIFLIDWWNIRKDIADKVLEDKGGEH
                     GNEAETSWLLYLYPELVHLEWADDGSINEPRFEELKNGWVWITRPWHLLTKNSGYGNP
                     KKATPEKGKAIVEGATDRIAEFLLKLSNAKIDDKFPY"
     gene            5172..6050
                     /gene="ribF"
                     /locus_tag="PLF90_06385"
     CDS             5172..6050
                     /gene="ribF"
                     /locus_tag="PLF90_06385"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOK56939.1"
                     /translation="MEIKHFSNIDIINKGISIGIGKFDGFHLGHKKIIEEVIKKAKNK
                     RLIPAVFTFKHFPVEFYISQWEEKIGFFENSGIELCLWCDYEEISFWEPFEFIEYLES
                     INVKCIVVGDNFKFGSHRKGGINDLQKKFEVSVLEAVKVDGETVNSSKIREFLKMGNF
                     ENVNKFLGRQFSFFGKVITGSSRGKNLGFPTANLSLLHNIKIGEGVYACWVKYKNIFL
                     KGALSIGNCPTFENEDTKIEVHIIGFNSVIYGEILEIFPVYRIREQKKYINIENLKYA
                     IRKDIEEINEKLTFPF"
     gene            complement(6052..6957)
                     /locus_tag="PLF90_06390"
     CDS             complement(6052..6957)
                     /locus_tag="PLF90_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOK56940.1"
                     /translation="MKEKFYGVYAALLTPFNENGNVCEKRLRKLVKFLISKGIDGLYV
                     CGSTGEFPLMDIEERKKVAEIVKEEVEDKLRIIVHIGSLSTKNTIKLAKHSEKIKVDA
                     VSSIPPFYYSFSFEEIFNFYKEVASSISLPFFFYYIPQTTGVTLKNEEILKLGEIKNI
                     IGLKYTYTDFYLLQDLLLKMKGKWIAFSGPDELFLPALTMGVIGSIGSTQNVLPEIFV
                     EIYKSFKNKDIDKASKLQKRVTEAVNLLHKYGGITSRKIALKFRGIDAGFAREPFLKD
                     LSNEKKEKFRKEWLEKFPEFSEGIK"
     gene            complement(6973..7935)
                     /locus_tag="PLF90_06395"
     CDS             complement(6973..7935)
                     /locus_tag="PLF90_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56941.1"
                     /translation="MEIKCIWWDGYHNAFTDIIKHKKNFLVTFRHATKHAVSGNGEIY
                     IIKSKNLEKWDLIKKFPNFHDSRDPKFFRFKNKIGVLFGVRFLNEKEKIDMYISYSED
                     GTNFIPPVKFENYNLWFWRIRNFNEKLYATSYELKLERGTYLFLSEDGLNFKEISQIV
                     KGEYANEADFLFEKDGLCYAIVRRENYQTPILAISTPPYKKWEKYPLNFIIQGPHIFK
                     FHDKIFTAGRIYLKKESLLSEIKDREYITKTGIFELDMKNKKLELVRILPSSGDTSYC
                     GSFYENGKLYLTYYSQHELNLKESKVGENASGIYLLITDYLNKK"
     gene            8028..9218
                     /locus_tag="PLF90_06400"
     CDS             8028..9218
                     /locus_tag="PLF90_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK56942.1"
                     /translation="MINVGIVGTGDRGTSFVKAIEKIPEMKVVAIVDTNPVRMEAVIR
                     HHNYPPCEKFTSIEELLDKKIADIIFITTPDWTHSDLIIKCLKKGYHVFCDKPLAINQ
                     KQIFEILKASNESDKMKFMGFNMRYKTLFRKMKEIVENEEIGKVLIGWSSSSYDGNGY
                     FRRWHKFREKSGGLIVHKGCHTIDILNWIIDSYPVEVYAKGGLSVFGGNKKWEGCHKC
                     EEINTCPYARKLSEKDEKLLEDIYIKAAVIDGYTRNYCVFAPTDVYDHYLVEITYENG
                     VKASFVEVFFGTGPFFMGFIGDKGEIITDSFIKNSIILRKKFGEEREKIFKVESEIKE
                     FHGGADLQMLKDIIESLNTGKQILPGFVEGARAAIIGIAAMISIDTGKPVQIKDLIPI
                     ELIS"
     gene            complement(9222..>11092)
                     /locus_tag="PLF90_06405"
     CDS             complement(9222..>11092)
                     /locus_tag="PLF90_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOK56943.1"
                     /translation="YPVISTEEKEKNHPILNFPYKLEENKELNGCLIFKKWSENQIAP
                     LRASADPNFAVMVIQENVCGKGKIIFNGIRRLYGHHRRGEIYASNIYSYVYGEEIKRI
                     PTADKRYRTKKEYEIWYKTPYSKFPFEEDAPEKNRIDKVDMKACINEVIGTNILITNG
                     KKGDLRFKVEMGEIKDEKRNIKIPEDRIKVMELEFDGSRMPDRMPERKEFVIGEGKTG
                     IIWINLNTNDLKEGDYSGDIVLVFEDGGERKVRLNIKIYPIELQKTNPIKLTVWDLVP
                     GGVGRDNMIKGAENWIKYHQDMREHGVNVFHLSAYERPGITFDEEGEIVKEDYTRFDS
                     GIPFKEKEYQYLINMGSHTEEFRIEGKKETVKYGTELWEKCYRNWVKSIIKHMKSIGL
                     DYSQFAFYPYDEIGTQTVPDALKIYSLIKEVDKKARIFVTIVPNGFFEAQKGLPVKEI
                     APYIDIWCPAFSHENYFSAGWPSKKEFDRILEFLKNTGKEIWSYNVLTRGNAEIMAYK
                     RYRLQPLSAYRMGIGGCGFYGYNLWKNDTYMVVYPDENPITSYRWEAMREGINDLKYI
                     ECLKKEMKKIKDEKKKKECEDLINEFLKEVTENNETPDLIYNYREKIVAKILELKK"
BASE COUNT         3983 a         1515 c         1606 g         3988 t
ORIGIN      
        1 ttgctcttct ttctctttca acttctgcct gtcttgccat tgctcttttc atagtatctg
       61 gtaattcaac ttcttttatt tcaacaatac ttacttttat tccccatggg tctgtctgtt
      121 catctattat ctgctgtaat ctgtgattta ttttttctct ttctgcaagt atttcatcaa
      181 gttcaagttg tcctgccact cctcttaatg ttgtttgagc aatctgcata gttgataatt
      241 gataattttc aacttcaaca actgatctta caggatccat aactctaaac cagacaacag
      301 cattaatttt tataggaaca ttatcttttg tcataacttc ctgaggggta acatcaaatg
      361 ttacagttct caaacttact tttaccattt tttctataat tggtataaga aaaattaaac
      421 caggacctct tgctccaacc aatctcccaa gacgaaaaac aactcccctt tcatattcat
      481 tcactatttt tatagcattt gctaaaatca taaaaatgaa aactgctaaa attatccatc
      541 ccataacttc ctcctttttg taaattgtat attataaaaa tggaattagt caaattaaag
      601 aatttgaaat ttttactttt ggatttataa tgtttcttta aaaaggtgta aaaatgttgc
      661 agaacaataa tttcaaaaat ggaaaatttg atatatattt taagtatctg agtgattaca
      721 aaaaggttgc tattcttgca ccattgttta tgattcttga agtaataatg gaccttttac
      781 agccaaaact tctatctact attattgatg atggaattgt taaaaataat ttaaagttga
      841 taataaatac tggttttttt atgtttttta ttgcaattat tggattgatt ggtgggattg
      901 gatgtacaat attttctagt atagcaagtc aaaattttgg acatgattta agaaaggata
      961 tttttaaaaa aatccagaat gcaaaattta aggaaataac caaattttct ccatcctccc
     1021 ttataacaag attaacaaat gacattgctc aatcacaaca acttgtttta atttctttaa
     1081 gaatgcttgt gagagctcct ttattatgta ttggtggtat tattatggct ttttcaataa
     1141 attttaaact ttcactgata atttttattg ttatgccttt gcttctctct attttttatt
     1201 ttatctctaa aaaaagtttt attttattta cagatatgca gaaaaaaatt gatagattga
     1261 atcttgttat aagggaaaat cttgcaggaa ttagagtaat aaaaattttt aatagatttg
     1321 catatgagaa aaagcggttt gaaaagtcaa atgaagattt aatggaagca tctcttaaag
     1381 caataaatct gataataaaa atgggaccac ttgtaatgat tatagtaaat ttaagtgtaa
     1441 taacagtatt atggtttggt ggaaaaatgg ttctaaccaa tgaaattaaa gttggagaaa
     1501 taatggcttt tattaactat ctgatgataa tacttatgtc tttgatgatg gtaagtaatt
     1561 tatttatttt tatttcaaga gcaggtgctt caatagaaag aattaatgaa gtactgaaaa
     1621 ttgaaggagt aaaggtttat gagaaatatt ctccattaga tatgataaaa ggaaaaatag
     1681 aatttaaaaa tgtttttttt agttatgatg ataattctga ttattatttt ttaaaagata
     1741 tttcttttaa aattgaagaa ggcgaaacag ttggaattat tggtactaca ggttcgggga
     1801 aaacaactat tttaaatctt attgcaggtt tatatgaacc tatttcaggt gaaattttga
     1861 ttgatgatgt gaatataaaa aatataaatc ctgataattt gaaaaaaaat ataggttttg
     1921 taactcagga aattgtaata ttttcaggta caataagaga aattatgaaa tgggcaagtg
     1981 aggaaacttc tgatgaagaa ataatagaag cacttaaaat tgctcaaata tatgattttg
     2041 tgagcaacct tccggaaggt cttgatactt ttataggaca gaaaggagtt aatctttcag
     2101 gaggacaaaa acagagattg acaatagcaa gagcgataat aaaaaaacca aaaattctta
     2161 ttcttgatga ttgtacaagt tcagttgatt acattacaga gaaaaaaata cttaaaaatc
     2221 tgaaagaaaa attgaataat tctactaaaa ttatagtaac tccaagaatt tttacaattg
     2281 cagaagcaga tagaatactt gtaattgata aaggtgaaat agtaggagaa ggaagacata
     2341 cagaattact tgaaaaatgt gaattttata gagaaattta tgaaacgcaa ataggggaaa
     2401 aaataaatgg ttgaaaaaga taaattttac gaacagcaaa aaattaagta tgtaagagga
     2461 catttttttg gaccaccggg tcctggaaga catatgaatt tattatcgga aaaaccaaaa
     2521 gaaacgataa aaacattaaa aaaacttttt aaatatttgg gaaaatataa actttacttc
     2581 cttctttctt ttgtttgtat attgggtagt tcaattttat ttctaatagg tccatattta
     2641 attggtaaag gaatagatta ttatataata cccaaaaatt taagtggatt tggaaaattt
     2701 ctacttttta tgttggttat atatatcttt aattccttac ttgtttatat tcagagttta
     2761 acaatggcaa atatttcaca aagagttggt tatgacttga gaaaagatgc ttttgaatgt
     2821 atccataaac tttttttgaa agtaattgac aaaagaacta taggtgacat tatgagcaga
     2881 ataacaaatg atatagatat gataaatatg actcttgcca caagtataac acaatttttt
     2941 tctggaataa ttatgctttc aggaacaatt gtaatgatga ttattttaag ccctctttta
     3001 acattattca gtatcatttt aattcctgtg atgattttta taacaaaaat aatatcagga
     3061 aaaacaagaa aatatttttt tattcaacaa acaaaacttg gagaattaaa tggaattgcg
     3121 gaagaagata taactggttt aagaataata aaaatttttg gtaaagaaga aaaagaaata
     3181 gaaaagtttg aaaaaattaa tgaagaatta aaagaatatg gaattaatgc tcagaaattt
     3241 gccggtatta tgcctccttt aatgaatttt ttaaataata taagttttgc aataattggt
     3301 ggaataggcg gtgtttttgt tttgaaaaaa ataattacaa ttggaacaat agccagtttt
     3361 attaattatg taaaacagtt tacaagacct ttaaatgaac ttgcaaatca attcaataca
     3421 attcaaactg caatagcaag tgctgaaagg gtttttgaaa ttatggaaga ggaaaaagaa
     3481 aaagaggatg aagaaggagc aatagaactt aaagaaataa aaggagaggt tgagtttaaa
     3541 aatgtatggt ttgcttatga agatgaaaaa tatgttttaa aaaatataaa ttttaaagtt
     3601 cctgctggga aaattattgc aattgttgga ccaacaggag caggtaaaac aacaataatt
     3661 aatttgttgg caagattata tgaagtagaa aaaggagaaa tacttattga tggatatgat
     3721 ataagaaaaa taaagaaaga aagtttaagg tcttttttag gtgttgtttt acaggataca
     3781 tatctttttg ctgaaacagt aaaagaaaat ataagatacg gaaaacttga agcaagagat
     3841 gaagaaattc aggaagtttg tagagtatca aatgctgaaa agtttatttt aaatctaccc
     3901 aaaaagtatc aaacagtttt gtctgatagt ggtcagagtt tgagtcaggg acagagacaa
     3961 ctcattgcaa tttcaagagc aattcttgct aaaccgaaaa ttttaattct tgacgaagca
     4021 accagcaaca tagatacaaa aactgagatg gatgtccaaa aaagtatgtt aaacttaatg
     4081 aaagataaaa caaactttgt tatagcacat agattgaata cagttaaaaa tgctgattta
     4141 attcttgtta taaaagaagg agaaattatt gaaaagggaa accataaaga attaatgaat
     4201 aaaaaagggt tttattacaa tctatttaca agtcaatttg gcgaagtttt aaatgattaa
     4261 aaatttacat aagaaaaaaa attgagtaaa attttattga attaaaatga aaagaaaact
     4321 ttctttatta agcataaaat ccttaaaatt atatattggt ggttttttta taaaaaggag
     4381 gatgatatga aatataaact tatggaaatg acattaaaag aagtaaaaga gtttggaaaa
     4441 gttgaagttt gtgttttacc ttggggttcc tgtgaaccac ataattttca tttgccatat
     4501 ggatgtgata cttttactgt tgaaaaaatt gcagaaataa gttgtaaaaa agcaaatgaa
     4561 aagggcggga aagtaatcct tcttccaaca atttcaatag gtgtcaattc aaatcatttt
     4621 ggttttccga tggttttaca tttatcacca acaactcaac tttctatact taaagatatt
     4681 gttaaaactc ttgaaaatta taaaatccat aaatttgttc ttattaatgg acacggagga
     4741 aatgaattta aaggtctttt aagagaatta tatggacaga caaaagtatt catctttctc
     4801 attgattggt ggaacataag aaaagatatt gctgataaag ttttggaaga taagggagga
     4861 gaacatggaa atgaagcaga aacaagttgg ttattgtatc tgtatcctga acttgtccat
     4921 cttgaatggg cggatgatgg aagtataaat gaaccgagat ttgaagagtt aaaaaatgga
     4981 tgggtctgga ttacaagacc atggcatctt ctcactaaaa attctggata tggaaatcct
     5041 aaaaaagcaa ctccagaaaa aggaaaagcg atagttgaag gagcaactga tagaattgct
     5101 gaatttcttc taaaattatc caatgcaaaa atagatgata aatttcctta ttaaaagaag
     5161 gagcaaaaaa tatggaaatt aaacattttt caaacattga tataataaat aaaggaatat
     5221 caataggaat tggaaagttt gacggttttc atcttggtca caaaaaaata atagaagaag
     5281 tgattaaaaa agcaaaaaat aaaagattaa taccggctgt ttttactttc aaacatttcc
     5341 ctgtagaatt ttatatttct caatgggaag aaaagatcgg tttttttgaa aatagcggaa
     5401 tagaattatg tctctggtgt gattatgagg aaatttcttt ctgggaacca tttgaattta
     5461 ttgaatatct tgaatcaata aatgtaaaat gtattgtagt tggtgacaat ttcaagtttg
     5521 gttcacatag aaaaggtgga ataaatgacc tacagaaaaa gtttgaggtt tcagtgttag
     5581 aggcagtaaa agttgatgga gaaacagtaa attcttcaaa gataagagaa tttttaaaaa
     5641 tgggaaattt tgaaaatgtt aataaatttc ttggtagaca gttttccttt tttggaaaag
     5701 ttataacagg aagttcaaga ggtaaaaatc ttggctttcc aactgcaaat ttatctcttt
     5761 tacataatat aaaaattgga gaaggtgttt atgcttgctg ggtaaaatat aaaaatattt
     5821 ttttaaaagg ggctttatca attggaaatt gtccaacatt tgaaaatgaa gatactaaaa
     5881 ttgaggtaca tattattgga tttaatagtg ttatttatgg tgaaattctg gaaatttttc
     5941 ctgtttatag aataagagaa caaaaaaaat atattaatat tgaaaactta aaatacgcta
     6001 ttagaaaaga catagaagaa ataaacgaaa aacttacatt ccctttttaa tttattttat
     6061 accttctgaa aattcaggaa atttttcaag ccattccttt ctaaattttt cttttttttc
     6121 attggataaa tcttttaaaa atggctctct tgcaaatcct gcgtcaatac ctctgaattt
     6181 taatgctatt tttctggatg ttatcccacc atatttatgc aaaagattta ctgcctctgt
     6241 aactcttttt tgtaatttac tcgctttatc aatatcttta tttttgaaac ttttataaat
     6301 ttccacaaat atttcaggca aaacattctg tgttgaacca atgcttccaa taactcccat
     6361 tgttaaagca ggtaaaaata attcatcagg accactaaat gcaatccatt ttcccttcat
     6421 ttttaaaaga aggtcctgta agagataaaa atcagtatat gtatacttca gtcctataat
     6481 attttttatt tcaccaagtt tcaatatttc ttcatttttt aaagttactc ctgtggtttg
     6541 aggaatataa tagaaaaaga atggaagaga aattgaagag gcaacttctt tataaaaatt
     6601 gaaaatttct tcaaaactga aactgtaata aaatggtggt attgaagaaa cagcatcaac
     6661 tttaattttt tctgaatgtt tagcaagttt tatagtattt tttgtactta aactacctat
     6721 atgaacaata attcttaatt tgtcttctac ttcttcctta acaatttctg caactttttt
     6781 tctttcctca atatccatca aaggaaattc tccagttgaa ccacatacat aaagtccatc
     6841 aatacccttt gaaattaaaa atttcacaag ttttcttaac cttttttcac atacatttcc
     6901 attttcatta aaaggtgtta aaagtgctgc ataaacacca taaaattttt ctttcacttt
     6961 ttttcctcct tttcattttt tattgagata atctgttatt aagagataaa tgccagaagc
     7021 attttctcca actttacttt cttttaaatt taattcatgc tgtgaataat atgttaaata
     7081 tagctttcca ttttcataaa aagaaccaca gtatgaagta tctcctgaac ttggaagaat
     7141 tcttacaagt tctaattttt tatttttcat atcaagttca aatattcctg tttttgttat
     7201 atattctcta tctttaattt cagaaagtaa actttctttt tttaaataaa ttctacccgc
     7261 agtaaatatt ttatcatgaa atttaaatat atgtggtcct tgaataataa aatttaaagg
     7321 atatttttcc cattttttat aaggaggagt agaaattgca agaattggtg tttgataatt
     7381 ttctcttctc actattgcat aacataaccc atctttttca aataaaaaat ctgcttcatt
     7441 tgcatattct cctttaacaa tctgtgatat ttctttaaaa tttaatccat cttctgatag
     7501 aaataaataa gttcctcttt caagttttaa ttcatatgaa gttgcgtaaa gtttttcatt
     7561 aaaatttctt attctccaga accagagatt ataattttca aatttgactg gtggaataaa
     7621 atttgttccg tcttctgaat aactgatata catatctatt ttttcttttt catttaaaaa
     7681 ccttacacca aataatactc ctattttatt tttaaatcta aaaaattttg ggtctctact
     7741 atcatgaaaa ttcggaaatt tttttattaa atcccatttt tccagatttt ttgacttaat
     7801 aatatatatt tcaccatttc cactaacagc atgtttagtt gcatgtctga aagttacaag
     7861 aaaatttttt ttatgtttaa ttatatctgt aaaagcatta tgataaccat cccaccatat
     7921 acattttatt tccatatttt tattttacaa tagatgtaaa aaaaaacaat tttttgacat
     7981 tattgtaaat aaaagtaaaa ttttaaatat tcaaagggag ggaaaaaatg ataaatgttg
     8041 gaattgtggg aactggtgat agaggaacaa gttttgtaaa agcaattgaa aaaattcctg
     8101 agatgaaagt agttgctatt gtagatacaa atcctgtaag aatggaagca gtaataagac
     8161 atcacaacta tcctccatgt gaaaaattta catctattga agaactactt gataaaaaaa
     8221 ttgctgacat tatatttata accactcctg attggacaca ttcagatttg ataataaagt
     8281 gtttaaaaaa aggttatcat gtattctgtg ataaaccact tgctataaat cagaaacaga
     8341 tttttgaaat tctgaaagca tctaatgaaa gtgataaaat gaaatttatg ggatttaata
     8401 tgagatataa aacattattc agaaagatga aagaaattgt tgaaaatgaa gaaattggaa
     8461 aagttttaat tggatggagc agtagtagtt atgatggaaa tggatatttt aggagatggc
     8521 ataaattcag agaaaaaagc ggaggattaa ttgttcataa aggttgtcat acaattgata
     8581 ttttaaactg gattatagat tcatatcctg ttgaagttta tgctaaagga ggactctctg
     8641 tttttggagg aaataaaaaa tgggagggat gtcacaagtg tgaagaaatt aatacatgtc
     8701 cctatgcaag gaaattatct gaaaaagatg aaaaattgct tgaagatatt tacattaaag
     8761 cagctgttat tgatggttat acaagaaatt actgcgtctt tgctccaaca gatgtttatg
     8821 accattacct tgttgagata acatatgaaa atggggtaaa agcatctttt gttgaggtct
     8881 tttttggaac aggaccattt tttatgggtt ttattggtga taaaggtgaa ataataacag
     8941 attcatttat aaaaaattca attatattaa gaaaaaaatt tggagaagaa agggaaaaaa
     9001 tatttaaagt tgagtcagag ataaaagaat ttcatggagg tgctgattta caaatgctta
     9061 aagatataat tgaatccctt aatacaggta aacagatttt gccaggattt gtagaaggag
     9121 caagagcagc aatcattgga atagcagcaa tgatatcaat tgacactgga aaaccagttc
     9181 aaataaaaga ccttattcca atagagttaa taagttgaaa tttatttttt aagttcaagg
     9241 atttttgcaa caattttttc tctataatta tatatcaaat caggagtttc attattttca
     9301 gttacctctt ttaaaaattc gtttattaaa tcctcacatt cttttttctt tttctcatct
     9361 tttatcttct tcatctcttt tttcaaacat tctatatact ttaaatcatt tatcccttct
     9421 ctcattgcct cccatctgta tgatgttatt ggattttcgt caggatatac aaccatataa
     9481 gtatcatttt tccataaatt gtatccataa aatccacatc ctccaatccc cattctgtaa
     9541 gcagaaagtg gttgtaatct atatctttta tacgccataa tttcagcatt tccccttgtt
     9601 aaaacattat aactccatat ctcttttcct gtatttttaa gaaattctaa tattctatca
     9661 aattcttttt ttgaaggcca accagcagaa aaataatttt catgtgaaaa agcaggacac
     9721 cagatatcta tataaggagc aatttctttt acaggtagtc ctttctgtgc ttcaaaaaat
     9781 ccattaggaa caattgttac aaaaattctt gcttttttat caacttcttt tatcagtgaa
     9841 tatatcttta aagcatctgg tactgtctgt gtccctattt catcatatgg ataaaaagca
     9901 aattggctgt aatcaagtcc tatacttttc atatgcttta ttatactttt tacccaattt
     9961 ctataacatt tctcccataa ctctgtccca tattttactg tctctttctt cccctctatc
    10021 ctgaattcct ctgtgtgtga acccatattg attaaatatt gatactcctt ctccttaaat
    10081 ggtatacctg aatcaaatct cgtataatct tccttcacta tctccccttc ttcatcaaat
    10141 gttataccag gtctctcata tgctgaaaga tgaaatacat taactccatg ctctctcata
    10201 tcttgatggt attttatcca gttctctgct ccttttatca tattatctct acctactccc
    10261 ccaggaacta aatcccatac tgtaagcttt atcggatttg tcttctgtaa ttctatcgga
    10321 tatatcttaa tatttaatct tactttcctc tctcccccat cttcaaacac caaaactata
    10381 tctccactat aatccccttc ttttaaatca tttgtgttta aatttatcca tattatacct
    10441 gtcttccctt ctcctatcac aaactccttc ctctccggca tcctgtctgg catcctgctt
    10501 ccatcaaatt caagttccat tacctttatc ctatcttcag gtatctttat atttctcttc
    10561 tcatccttta tctcccccat ctctacttta aatcttaaat cccctttctt tccatttgtt
    10621 ataagtatat tcgtcccgat tacctcattt atacatgctt tcatatctac tttatctatc
    10681 ctgttttttt caggtgcatc ctcctcaaat ggaaatttac tgtatggcgt cttataccag
    10741 atttcatatt ctttttttgt cctgtatctt ttatctgctg tcggtatcct ctttatctcc
    10801 tctccataaa cataagaata aatatttgaa gcatatattt ctcctcttct gtgatgtcca
    10861 tataatcttc ttattccatt aaaaataatt ttcccttttc cacatacatt ttcctgaatt
    10921 accataaccg caaaattcgg gtctgctgat gcccttaatg gcgctatctg attttctgac
    10981 catttcttaa atataagaca tccattaagt tctttattct cctcaagttt atatggaaaa
    11041 tttaaaattg gatgattttt ttctttctct tcagttgata taacaggata aa
//
LOCUS       DAOSAT010000063        10898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_3994_length_10898_cov_4.191368, whole genome shotgun sequence.
ACCESSION   DAOSAT010000063 DAOSAT010000000
VERSION     DAOSAT010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3994_length_10898_cov_4.191368"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="PLF90_06410"
     CDS             <1..1176
                     /locus_tag="PLF90_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56944.1"
                     /translation="KKTIEKEKQLQLIEITRKIKEERKEHLIKEKPKVKQVKEEKIKH
                     EEIKIKKPLKEEILKKRAEQQKIKAYIGVISKIVSETVKPLESQLNDAKKNISNFINI
                     TKKEIKKKKPPKVKPEKVKITPKEKIKVSAYKEPFKLNVFLRRNIFKFVFLALLLVWL
                     IEVFMYLKKLISPEERLKLIVGETIEERPAIEKEKEVKEEEKEEKLVYYTKEKIDIEG
                     KRDPFSTGRLTMEVMKKPTPTNIIYAKKPDVISIIKTQKFVSILKPEEKITSPEIPTI
                     TKPSPVEKPSITKVSPTEKPSISKPEQLSTPTKLTEIEKVPEVKITPFVAPEKECKLV
                     YRGRMILEGVEYFFIEGEKKTYKVTVGDEIEGYRILKKQDKQIVLSKDGILYEINIK"
     gene            1225..3081
                     /locus_tag="PLF90_06415"
     CDS             1225..3081
                     /locus_tag="PLF90_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56945.1"
                     /translation="MLKKVILFLFFLTIIYCFGENENLEDLYRLGVSYYYKKDYPNAI
                     ATFNKIKGINPGYRKSQVDRYIRVSETKLGKLNPKTEFKIGEDLKGEIVVNREDEFEK
                     MAFQAQQAIIETSRYFNEVEEKYNLTDLEKLEPTSTFNLAKKAYESNMYTEAIRLASK
                     ARRQIEEIVLRKLEEERPKLGEIGNKRVTIELKDMNLKEALDELYNITGVSYILSPNV
                     TGKVTLSFKDIPIKIVLDAICEQNGLKYIEENGIIKIMTKAEYQEKMKEIQKARRVFK
                     LYYTDANIIVKALREVFKGIDAFPEPTTNSIIVEAKSENILRNIEGVISTLDTPVSQV
                     LLECKIVEVAVSEGNQFSIDWMISSRMIDAIDGTLTGPRFGDNINYTPGLTQTLPSGF
                     SFGVTNRDFNALIQALATRGKARVIQAPKITCINGTTAYIAVTQNEPYIIPETQQTIT
                     TPATGAPTVTTASAINLQEEIIGTEFEVTPLIQRNRTVMLNLNIYDSNLIERRKLEAT
                     TADQRYSIEIPILSERMTTQSVVLYDKQTLVIGGMIRGREEAKESGIPFLRRIPILGY
                     LFKKPTFDRTKSELLIFLTPHIISSYEEAEEISKPDVKKSEEKIEGGLLEKF"
     gene            3160..4224
                     /locus_tag="PLF90_06420"
     CDS             3160..4224
                     /locus_tag="PLF90_06420"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOK56946.1"
                     /translation="MVKKIVSLMIILMVATVFSQTSTQQATRISNISRQGSIGSRTGT
                     SGQRGSGTGTGFGIRGQSGNQQQDYSSSQLANFLAAIIGSYLRHPDPKVRIQAIQSLT
                     GAMVSGEGTGNNTNNNSGIRGLFGIDVNNTGNNNTTSGGIGGAIFIPDLFVLLSDPDP
                     EVQDMASMGLDILFGTDVTILRFMDDPDPIVRQYATKIYSTRSFSGDTNRTTGNNNNE
                     YEEGSLKELVALRTLLVRLKYEKDEGVKKVLTDSIELYLQSIGLGREGQSGTRGTSLT
                     GIFGVDPSIIKYLNDDNVEVRKNAVKIISAMEYNPQILNILLERLKKETDDGVKEAIQ
                     NAIDSYTQQKSRALGTQGLR"
     gene            4251..5843
                     /locus_tag="PLF90_06425"
     CDS             4251..5843
                     /locus_tag="PLF90_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOK56947.1"
                     /translation="MKKFLFSFIYFFSFSFLLLCENNFYLNFNNVDIRTFIKFVSEIT
                     KENYVIDPNVRGTITIYSTTPIPVEQIDRVFKAVLNFYGFTTIKKDNISLIVPISDGK
                     TRTRYINISDIPKDKEEEYIIQVISLKYYPPDTISQIITPYITKGGQITVDTRTNTLI
                     ISDVGENIKKIMEIIEKIDKPSPPGQEEIRIFKLQNADAEEVAKVLGQILQRKISAVR
                     PSARTAPIQPQVVSAKATNSLIIYADPNDFDTIEKIIKELDILTNQVLIEALIAEVTF
                     DKTQQLGIEWANSWKFNNEQYTGAVGTDFGDIASYIKSGVPPSGLSLAFYKGDFTYPL
                     SVGALINLYAKDSQFNILSTPQIVTADNQEAKINISENIPYLKETRFYTYGSGQGSDV
                     VKSYDYKDVGIVLKITPQISQDKYVKLKISQEVTKLVEGGIPEAPTTAKRQAETTLII
                     PNGKTVVLGGLLRNDSEKSVQKVPFLGDIPLLGRLFRKDSTKSIKTNLMIFITPHIIS
                     TFEDAEKIKQEKEKLIKEKNNG"
     gene            5836..7359
                     /gene="gspE"
                     /locus_tag="PLF90_06430"
     CDS             5836..7359
                     /gene="gspE"
                     /locus_tag="PLF90_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOK56948.1"
                     /translation="MDRVMWIENFEDRIDPEILNVFPYNFLKTNCFIPINGENGEIII
                     VTNNPLNLDIGEIKTILKKDIKIALSSDEKINECIEKFYYHPSSSKKVIDDLSSEEIL
                     HIEEDINTETANLLDLANKAPVIRIVNQIIYRAISMRASDIHLQITENSLKVRYRIDG
                     ILHDIFVLPKKFHPPIVTRIKIMSNLDIAEKRIPQDGRTSIKVDGKKIDIRISIIPTF
                     FGESIVLRLLDRATFLLGLEELGFSEDNYKKFNQLIHCDHGIILLTGPTGSGKTTTLY
                     AALSKINNPGINIVTLEDPVEYNLEGINQIQVNHKVGLTFANGLRSILRHDPNVIMVG
                     EIRDKETAEMAIQASLTGHLVFSTLHTNDASSAIVRLIDMGIETYLLSSSLIGVMAQR
                     LVRLNCPNCSIPENPPVELLSEIGISEDEIVNSKFMKGKGCKQCLNEGYYGRTGIFEL
                     LIIDEGIKELILKRASSNDIKKEGLKKGMKTLRMDGIEKVKKGLTTISEVLRVTQDV"
     gene            7361..8557
                     /locus_tag="PLF90_06435"
     CDS             7361..8557
                     /locus_tag="PLF90_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOK56949.1"
                     /translation="MPTFIFTLIDKKGKVIKIKKDGENVNTLKNEFQKEGYYVVNVKE
                     FKKFNFSIFDKIREDDLIVAIRELSTFLNSKIPLDECLTGLISQMKDGKLKNVFLDIQ
                     KSIREGASFSQALKKHSNLFSNMIISMVRVGEETGNLDLVLLRVSDFLEKRNTFKNKI
                     KTIMTYPVFMIVVAFFVVIFILSFIIPTITRIFSEISINLPFLTKILIRVANFFRHFW
                     IHFVLFVIFISLLIKNFFNTKKGKIIFENILWKIPFLNDIFLKRELINFSKTLSTLIN
                     GGVDIIESLNIGKEVLNSIYIKKEVEELTEYVSKGGSLSSGFSKSKYFPYLFTQLLGA
                     GERSGNLSEMLDKIGDIYQEELSQKSTRFVTFLEPFMILFMGGIIGFIVISVLLPIFQ
                     ISQTIK"
     gene            8566..9000
                     /gene="gspG"
                     /locus_tag="PLF90_06440"
     CDS             8566..9000
                     /gene="gspG"
                     /locus_tag="PLF90_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOK56950.1"
                     /translation="MNMKKGFTFIELMVVIIILGLLAMLVFPSFLRRVDDAKITAAKV
                     QIKSFESGLKLFYLDNGFYPSTEQGLKALIEKPTTGRIPQKWREGGYLDKPSIPKDPW
                     GNDYIYLSPGRNGEDYEIISYGRDGKEGGEGPDADISSSEIE"
     gene            complement(9006..9188)
                     /locus_tag="PLF90_06445"
     CDS             complement(9006..9188)
                     /locus_tag="PLF90_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56951.1"
                     /translation="MKKKYTLHLFESANFSASLILSIILFNSSSATSGRKDLTEGKIR
                     EKKTKLSTEPLSFQKM"
     gene            9449..9832
                     /locus_tag="PLF90_06450"
     CDS             9449..9832
                     /locus_tag="PLF90_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOK56952.1"
                     /translation="MKTFKKGFTFLEIMIAVGIFGIVSIVCITNYLICLKEIKIVNDK
                     IKMVILAEQKIEELKLKNEEIKEEEGNFNDPDSDYIWEIKLSDIVIYDTEENIELIPY
                     KLTIKSQHDYYSLIIPFLKMQEKNE"
     gene            9825..10370
                     /locus_tag="PLF90_06455"
     CDS             9825..10370
                     /locus_tag="PLF90_06455"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOK56953.1"
                     /translation="MNKFKKNGFTLIEILISLLLILITFLLVYRTYFTIQKNMKEVEK
                     EVKNNEILFNFLYSFKSELENIFNIENLKFDKKEVTFTAFIQDIDFPVEITYTVKNNE
                     KEEVLIRTQKNLFSGYTYSFPVLTCESINFLVFQNENWEYFIESDKVAEGIAIEIIYP
                     EKRIFYPVLLNIEKKLENEKK"
     gene            10357..>10898
                     /locus_tag="PLF90_06460"
     CDS             10357..>10898
                     /locus_tag="PLF90_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOK56954.1"
                     /translation="MKKNKGFILIYVLGFSLALTSVILIFNFKTKKYIESFKRNYQIF
                     EMEKIADIGFEIAKKIIEKDTNNYDWIKEKWAEEKNFNIENYELNILIEDENSKININ
                     KILEKEGKTNQLLLEVLKNLFVILEYPSSLLDCLLDWMDEDSIPKPSGAEDIYYSSIG
                     LNYAPPNRNLLTLKELYYIKG"
BASE COUNT         4517 a         1284 c         1778 g         3319 t
ORIGIN      
        1 aaaaaaacta tagaaaaaga aaaacaactt caacttatag aaataacaag aaaaattaag
       61 gaggagagga aagaacactt aataaaagaa aaaccaaaag taaaacaggt aaaagaagaa
      121 aaaataaaac atgaggaaat aaagataaag aaacctttaa aagaagagat attgaaaaag
      181 agggcagaac agcaaaaaat taaagcatat ataggtgtta tttctaaaat agtaagtgaa
      241 actgttaaac cattagaatc tcaattaaat gatgctaaaa agaatatttc aaattttata
      301 aatattacta aaaaggaaat caagaagaaa aaacctccaa aagtcaaacc tgaaaaagta
      361 aaaattactc caaaagaaaa aattaaagtt agtgcatata aagaaccatt taaattaaat
      421 gttttcttaa gaagaaatat atttaaattt gtttttttgg ctcttttact tgtatggctt
      481 atagaagttt ttatgtattt gaaaaaatta atttcaccgg aagaaagatt aaaacttata
      541 gttggagaga cgattgaaga aagacctgca atagaaaagg aaaaagaagt aaaagaagaa
      601 gaaaaagaag aaaaacttgt ttactacaca aaagaaaaaa ttgatattga aggaaaaaga
      661 gacccatttt ctacgggaag attaacaatg gaagttatga aaaaacctac tcccacaaat
      721 attatatatg ctaaaaaacc cgatgtaatt tcaattataa aaacacaaaa atttgtcagt
      781 attttaaaac cagaggaaaa aattacatct cctgaaatac caactattac aaaaccttca
      841 ccggtagaaa aaccgtctat tacaaaagta tcacctacag aaaaaccttc tatttctaaa
      901 cctgaacaac tttccactcc tacaaaatta actgaaattg aaaaagtccc tgaagtgaaa
      961 attacaccat ttgttgctcc tgaaaaagaa tgcaaacttg tttatagagg caggatgatt
     1021 ttagaaggtg ttgaatattt ctttattgaa ggagaaaaga aaacatataa ggtaacagta
     1081 ggtgatgaaa tagaaggata tagaatattg aaaaaacagg acaaacaaat tgttttatct
     1141 aaggatggaa tattatatga aattaacatt aaataacttg actaacttta aaaaaatggg
     1201 taagattaaa aaaggaggga gttaatgtta aaaaaagtaa ttttattttt gttttttctc
     1261 accataatat attgttttgg agaaaatgaa aatcttgaag acctttatag attaggtgtt
     1321 tcttattact ataagaagga ctatccaaac gcaattgcta cttttaataa gataaaggga
     1381 ataaatccgg gttatagaaa atctcaggtg gatagataca ttagggtctc tgaaacaaaa
     1441 cttggcaaat tgaatccaaa aacagagttt aaaataggag aagatttaaa aggagaaata
     1501 gttgttaata gggaagatga atttgaaaaa atggcatttc aggcacaaca agcaattata
     1561 gaaacaagca gatattttaa tgaagttgaa gaaaaataca atcttacaga tttagaaaaa
     1621 ttagaaccaa cttctacttt taatcttgct aagaaggcat atgaaagcaa tatgtataca
     1681 gaagcaatac gacttgcaag taaagcaaga agacagatag aagaaattgt tttaagaaaa
     1741 ttagaagaag aaagaccgaa attgggtgaa attggaaata aaagagtaac catagaatta
     1801 aaagatatga atcttaaaga agcacttgat gaattatata atattacagg agtgagttat
     1861 attttaagtc caaatgttac tggaaaagtt acactcagtt ttaaagatat acctatcaaa
     1921 atagtacttg acgcaatatg tgaacagaat ggtcttaaat atatagaaga aaatggaata
     1981 ataaaaataa tgacaaaagc agaatatcag gaaaaaatga aagaaataca gaaagcaaga
     2041 agagttttca aactgtatta tacagatgcc aatataattg taaaagcatt aagagaagta
     2101 tttaaaggta tagatgcatt cccagaacca accaccaatt ccattattgt tgaggcaaaa
     2161 agtgaaaaca ttttaagaaa tatagaaggt gttatttcaa cacttgatac acctgtttct
     2221 caggtacttc ttgaatgtaa aatcgttgaa gtagcggttt ctgaaggcaa tcaattttca
     2281 attgactgga tgataagttc aagaatgata gatgctattg atggaactct aacaggccca
     2341 agatttggag ataacataaa ctatactcct ggtttaacac agacattacc ttctggattt
     2401 tcttttggag taactaatag ggattttaat gcattgattc aggcgcttgc aacaagagga
     2461 aaagcaagag taattcaggc accaaaaatt acctgtataa atggaacaac tgcatatatc
     2521 gcagtaacgc aaaatgaacc atatataatt ccagaaacac agcaaacaat tacaacacct
     2581 gcaactggag caccaacagt aaccactgca agtgcaataa atcttcaaga agaaataata
     2641 ggaacagaat ttgaagttac accattgata cagagaaata gaacggttat gcttaatctg
     2701 aatatatatg actcaaatct aattgaaaga aggaaacttg aagcaacaac tgcagaccag
     2761 agatattcaa ttgaaatccc aattttatct gaaagaatga caactcaaag tgtagttctt
     2821 tatgataaac agacacttgt aattggaggt atgataagag gaagagaaga agccaaagaa
     2881 tcaggtatcc cgtttttaag aagaattccc attcttggtt atttatttaa aaaaccaact
     2941 tttgatagaa ctaaaagtga acttcttata ttcttaacac cccacataat ttcatcttat
     3001 gaagaagcag aagaaatttc aaaaccagat gttaaaaaat cagaggaaaa aatagaagga
     3061 ggattacttg agaaatttta aaattaaagt attgacattt ataaaaaata aaggtaatat
     3121 aaaaatgaaa aaagagaaaa taaaaaaaag gaggggaaaa tggtaaaaaa aatagtaagt
     3181 ttaatgataa tattgatggt agcaactgta ttttctcaaa cctcaactca acaagcaaca
     3241 agaatttcaa acatatccag acaaggaagt attgggtcaa gaacaggaac ttcaggacag
     3301 agaggatctg gaacaggtac aggatttgga attagagggc aatcaggaaa tcaacagcag
     3361 gactacagta gttcgcaact tgcaaatttc ctggcagcaa taattggaag ttacttaaga
     3421 catcctgacc caaaagtaag aattcaggca atccaatcat taactggggc aatggtatca
     3481 ggagaaggaa caggaaataa tacaaataat aatagtggaa taagaggact atttggaata
     3541 gatgtcaaca atacaggaaa taataataca acatctggtg gaataggagg agcgattttt
     3601 attcctgacc tttttgtgct tttaagtgac cctgaccctg aagttcaaga tatggcatca
     3661 atgggtcttg atattttatt tggaacagat gttactattt taagatttat ggatgaccct
     3721 gatcctattg taagacaata tgcaactaaa atttattcaa caagaagttt ttcaggagat
     3781 accaatagaa cgactgggaa taataacaat gagtatgaag aaggaagttt aaaagaactt
     3841 gttgctttaa gaactttact tgtaagatta aaatatgaga aagacgaagg agtgaaaaaa
     3901 gtactaactg actcaataga attatattta cagagtattg gattgggtag agaaggacaa
     3961 agtggaacaa gaggaacaag tttaacagga atttttggag tagacccatc tataataaaa
     4021 tatcttaatg atgataatgt agaggtaaga aaaaatgctg ttaaaataat ttctgctatg
     4081 gaatataatc ctcaaatttt gaatatattg cttgaaagat taaagaaaga aaccgacgat
     4141 ggagtaaaag aagcaattca gaatgccatt gattcctata cacagcaaaa aagtagagca
     4201 ctgggaactc aggggcttag ataaaaatag aattcagaaa taaaataaaa atgaaaaagt
     4261 ttttatttag ttttatttat tttttttctt tctcattttt gttattatgt gaaaataatt
     4321 tttatttaaa ttttaataat gttgacataa gaactttcat taaatttgtc agtgaaatca
     4381 caaaagaaaa ctatgtaata gacccgaatg ttagaggaac aataactatt tattcaacta
     4441 caccaattcc tgttgaacaa attgatagag tttttaaagc agtactgaat ttttatggat
     4501 ttacaactat aaaaaaagat aacatttctt taattgtacc gatatctgat ggaaaaacaa
     4561 gaacaaggta cataaatata tcagatattc caaaagataa agaggaagaa tatataattc
     4621 aggtgatttc tttaaaatat tatcccccag atacaatttc tcagataatt acaccataca
     4681 ttacaaaagg agggcaaatt acagttgata caagaacaaa tactttaatc atttcagatg
     4741 ttggagaaaa tataaaaaaa atcatggaaa ttattgaaaa aatagataaa ccaagtcctc
     4801 ctggacagga agaaataagg atattcaaac tacaaaatgc agatgctgaa gaagttgcaa
     4861 aagtacttgg tcaaatatta cagagaaaaa tttctgctgt tagaccatct gcaagaactg
     4921 caccaattca acctcaggta gtaagtgcaa aagcaacaaa ttcattaata atttatgcag
     4981 accctaatga ttttgatact attgaaaaaa taataaagga acttgatatt ttaacaaatc
     5041 aggttttaat tgaagcatta atagcagagg tgacttttga taaaactcag caacttggaa
     5101 tagaatgggc aaattcttgg aaatttaata atgaacaata tacaggtgca gtgggaacag
     5161 attttggaga tattgcttct tatataaaat caggtgttcc accatcagga ctttctcttg
     5221 ctttctataa aggtgatttt acctatcctt taagtgttgg tgctttaata aacttatatg
     5281 caaaagacag tcaattcaat attctctcca ctccacagat tgtaacagca gacaatcagg
     5341 aggcaaaaat aaatattagt gagaatatac cttatttaaa agaaacaaga ttttatacct
     5401 atggttccgg tcagggtagt gatgtagtta aaagttatga ttataaagat gtaggcattg
     5461 ttttgaaaat tactcctcag ataagtcagg ataaatatgt gaaattaaag atttctcagg
     5521 aagttactaa acttgttgaa ggaggaatac ctgaagcacc aacaactgca aaaagacaag
     5581 cagaaacaac attaataatt ccaaatggaa aaactgttgt tcttggaggc cttttaagaa
     5641 atgattcaga aaaatctgtt cagaaagtcc cgtttttagg agatatacca ttgcttggac
     5701 gattattccg aaaggactca acaaaatcta taaaaacaaa tttaatgata ttcattactc
     5761 ctcatataat ctcgactttt gaagatgcag aaaaaataaa acaggaaaaa gaaaaactta
     5821 taaaggaaaa aaataatgga tagagttatg tggattgaaa attttgaaga caggatagac
     5881 cctgaaattt taaatgtttt tccatataat tttcttaaga caaactgttt tatacctata
     5941 aatggagaaa atggagaaat tataatcgtt acaaataacc ctttaaatct tgatataggt
     6001 gaaataaaaa caattttaaa aaaagatata aaaattgctc tttcaagtga tgaaaaaata
     6061 aatgagtgta ttgagaagtt ttattatcat ccaagttctt ctaaaaaagt tattgacgac
     6121 ctcagttcag aagaaatatt acacatagaa gaagatatta atacagaaac agcaaatcta
     6181 cttgaccttg ccaacaaagc acctgttatt agaattgtaa atcaaataat ttatagagca
     6241 atatctatga gagcaagtga tattcattta cagattactg aaaactcttt aaaagtaaga
     6301 tataggattg atggtatctt gcacgatatt tttgttcttc ccaaaaaatt tcatcctcct
     6361 atcgtaacga gaataaaaat aatgtctaat cttgatattg cagaaaaaag gattccacag
     6421 gatggaagaa cttctataaa agttgatggt aaaaaaattg atataagaat atcaataata
     6481 cctacttttt ttggagaatc aattgtttta agattacttg atagggcaac ttttttactt
     6541 ggtcttgaag aactcggttt ttctgaagat aattacaaaa aattcaatca attaatccac
     6601 tgtgaccatg gtattatttt attaacaggt ccaaccggaa gtggaaaaac gacaactctt
     6661 tatgctgctt tatctaaaat taataatcca ggaataaata tagtaactct agaagaccct
     6721 gttgagtata atcttgaagg gataaatcaa atacaggtaa atcacaaagt aggtttaacc
     6781 tttgctaatg gattaaggtc aattttaaga cacgacccaa atgtaataat ggtgggagaa
     6841 ataagagata aagaaacagc ggaaatggca atacaggcat cattaaccgg acatcttgtt
     6901 ttcagtaccc ttcatacaaa tgatgcttca agtgctattg ttagattgat agatatggga
     6961 attgaaacat atttactttc atcctcttta ataggagtta tggctcaaag gttggttaga
     7021 ttaaattgcc caaattgttc tattcctgaa aatcctccgg tagaattact ttctgaaatt
     7081 ggtattagtg aggatgaaat tgtaaatagt aaatttatga aagggaaggg atgtaaacag
     7141 tgtttaaatg agggttatta tggaagaact ggtatttttg aactccttat tattgatgaa
     7201 ggaataaagg aacttatatt aaagagagca tcatcaaatg acatcaaaaa agaaggattg
     7261 aaaaaaggaa tgaaaacatt gagaatggat gggatagaaa aagttaaaaa agggcttaca
     7321 acaatttcag aagttttaag agtaacacag gatgtttaaa atgcccacct ttatatttac
     7381 tctgatagat aaaaaaggaa aagtaataaa aataaaaaaa gatggtgaaa atgtaaatac
     7441 tttaaaaaat gaatttcaaa aagaaggata ttatgttgta aatgtaaaag agtttaaaaa
     7501 atttaatttt tcaatttttg ataaaataag agaagatgac ttaattgttg caataagaga
     7561 attatctact tttttaaact ctaaaatacc tcttgacgaa tgtcttacag gattaatttc
     7621 acaaatgaaa gatggaaaat tgaaaaatgt ttttcttgac atacaaaaaa gtataagaga
     7681 gggagcgagt ttttctcaag cattaaaaaa acattcaaat ttattttcaa atatgattat
     7741 atctatggta agagtaggtg aagaaacagg aaatctggat ttagttttat taagagtatc
     7801 tgattttctt gaaaaaagaa acacatttaa aaataaaata aaaacaatta tgacatatcc
     7861 ggtatttatg atagttgttg ctttttttgt tgttattttt attctttcat ttattattcc
     7921 aacaattaca agaatctttt ctgaaatatc aattaattta ccttttttaa caaaaatatt
     7981 aatccgagtt gctaattttt tcagacattt ctggatacat tttgtccttt ttgttatttt
     8041 tatttcatta ctcataaaga atttttttaa cacaaaaaaa ggaaaaataa tatttgagaa
     8101 tattttatgg aaaataccat ttttaaatga tattttttta aagagagaac ttattaattt
     8161 ctcaaaaaca ctttctactt taataaatgg aggggttgat attattgaat ccctaaatat
     8221 aggaaaagaa gttttaaata gcatttatat taaaaaagag gttgaagaat taacagaata
     8281 tgtttcaaaa ggtggttccc tttcttccgg tttcagcaaa agtaaatatt ttccatattt
     8341 attcacacaa cttttaggtg ctggagaaag aagtggaaat ttatctgaaa tgcttgataa
     8401 aataggagat atttatcagg aagaactttc tcagaaatca acgagatttg taacatttct
     8461 ggaaccattt atgattcttt ttatgggagg tataataggt tttattgtta tatcggtttt
     8521 attgccaatt tttcagatat cacaaacaat aaaatgaggt aaaaaatgaa tatgaaaaag
     8581 ggtttcacat ttattgaatt gatggtagtt atcataatcc ttggtttact cgcaatgctt
     8641 gtatttcctt cttttctgag aagagttgat gatgcaaaaa ttacagccgc aaaggttcag
     8701 ataaaaagtt ttgaaagtgg acttaaatta ttttatcttg ataatggttt ttatccatct
     8761 acagaacaag gtctaaaagc attgattgaa aaaccaacta cgggtagaat tcctcaaaaa
     8821 tggagagaag gtggatatct tgacaaacca tctattccaa aagacccatg gggtaatgat
     8881 tatatatatt taagtccggg tagaaatgga gaagattatg aaattatatc atatggaaga
     8941 gatggaaaag aaggtggaga aggtcctgat gcagatatat caagttcaga aattgaataa
     9001 gggttttaca ttttttgaaa tgatagtggt tctgttgata atttcgtttt tttttctcta
     9061 attttaccct cggtcaaatc ttttctaccc gaagttgctg atgaagaatt aaataatata
     9121 atagataaaa ttaaagaagc agaaaaattt gcagattcaa atagatgtaa tgtatatttc
     9181 tttttcaatc ttgacgaaaa ttcttactgg tttgaaggtg acattgaaga aaaaaatcag
     9241 gttgaaccag ttgtttcaaa tcttgatttt tatgaaggaa aaaatgaaat agagaaaaaa
     9301 aacagtggaa taataaattt taaaatttca cctgatggga aaaaagattt ttttatctta
     9361 tatatttatg atgatgataa agatttaaaa tacactcttt tcagtaatcc tttttttgat
     9421 atagaaattt taaaaggaga aataaacgtt gaaaacattt aaaaagggtt ttacttttct
     9481 tgaaataatg attgctgttg gcatttttgg gattgttagt atagtttgta taactaatta
     9541 tcttatatgt ttaaaagaaa taaaaattgt aaatgataaa ataaaaatgg taattttggc
     9601 agaacaaaaa atagaagaat tgaaattgaa aaatgaagaa ataaaggaag aagagggaaa
     9661 ttttaatgac cctgatagtg attatatatg ggaaataaaa ctttctgata tagtaattta
     9721 tgatacagaa gagaatattg aactaattcc ttataaatta acaataaaaa gtcaacacga
     9781 ttattattct ttaattatcc cttttttaaa aatgcaggaa aaaaatgaat aaatttaaaa
     9841 aaaatggatt tacattaata gagattttaa tctcgctttt actaatctta atcactttct
     9901 tacttgttta cagaacttat tttacaattc aaaaaaatat gaaagaagtg gaaaaagaag
     9961 taaaaaacaa tgaaatttta ttcaattttc tatattcttt taaatcagaa ttagaaaata
    10021 ttttcaatat tgaaaattta aaatttgata aaaaagaagt tacgtttact gcttttattc
    10081 aggatataga ttttccagtt gaaataacat ataccgtaaa aaataatgaa aaagaagaag
    10141 ttttaataag aactcaaaaa aatttattct caggatatac atatagtttt cctgttttaa
    10201 catgtgaaag tataaatttt ttagtttttc agaatgaaaa ctgggaatat tttatagaat
    10261 cggataaagt tgctgaaggt attgctattg aaataattta tcctgaaaaa agaatttttt
    10321 atcctgtttt acttaatatt gaaaaaaaac tagaaaatga aaaaaaataa aggttttatt
    10381 ttaatttatg ttcttggttt ttcacttgca cttacatctg tgattttaat ttttaacttt
    10441 aaaactaaaa aatatattga atcatttaaa agaaattacc aaatatttga gatggaaaaa
    10501 attgctgata taggatttga aattgcaaaa aaaattattg aaaaagacac aaataactat
    10561 gattggataa aagaaaaatg ggcagaagaa aaaaacttca atatagaaaa ttatgaattg
    10621 aatatattaa tagaagatga aaactcaaaa ataaatatta ataaaatttt agaaaaagaa
    10681 ggtaaaacta atcaacttct tcttgaagtt ctaaaaaatt tatttgtaat ccttgaatat
    10741 ccttcctcac ttcttgattg tttacttgac tggatggatg aggatagtat accaaaacct
    10801 tcaggtgcag aagatattta ttattcatca attggattga attatgctcc tccaaataga
    10861 aatcttctga ctcttaagga actttattat ataaaagg
//
LOCUS       DAOSAT010000064        10588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_4144_length_10588_cov_5.434729, whole genome shotgun sequence.
ACCESSION   DAOSAT010000064 DAOSAT010000000
VERSION     DAOSAT010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10588
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4144_length_10588_cov_5.434729"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLF90_06465"
     CDS             complement(<1..522)
                     /locus_tag="PLF90_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56955.1"
                     /translation="MNLKVPLEKPEPDFEELKNVLLGNQKAERVHFVELLMDYEMKKY
                     ITEKIIGEKWIEYSEENKEKYIKQEINFWYKLGYDYVRIAGGIIFTGKSRRGKDTAIL
                     SKGERGWVEEGTGMITNWSDFENYPWQKKEEIDYSLYEIASKNLPDGMKMLVCPSSGV
                     FEIASEVLLGFENM"
     gene            complement(574..665)
                     /locus_tag="PLF90_06470"
     tRNA            complement(574..665)
                     /locus_tag="PLF90_06470"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(629..631),aa:Ser,seq:cga)
     gene            complement(652..1329)
                     /locus_tag="PLF90_06475"
     CDS             complement(652..1329)
                     /locus_tag="PLF90_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOK56956.1"
                     /translation="MVNSKVSIKERVQKILKEVPENVILLAATKGRTVEEIDEAIEGG
                     IKFIGENYVQEAEKKFIKIGKKVKWHMIGHLQTNKVKKAFEIFDMIETVDSLKLIREI
                     DKEAKKKKVIYPVLIEINSGRELQKSGVLPEKVEELIYEISKFENVRVEGLMTMGPFV
                     ENPEDIRPYFKLTKDLFEKLKNLNLPNVEMKYLSMGMTDTWKIAIEEGANIIRIGTGI
                     FGPRRGG"
     gene            1364..3025
                     /locus_tag="PLF90_06480"
     CDS             1364..3025
                     /locus_tag="PLF90_06480"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOK56957.1"
                     /translation="MFSIGIDFGTNSVRGIILNLKNGEIVSEKVVPYPSGIDGVIIDE
                     KDPHLARQNPADYHICLEKLLKEIIKKAKKNKSFSVDKIVGIGIDTTGSTPMPVDKNL
                     VPLSFYKEFKNNVNAMAWLWKDHTSSDEAEEITELSKKIRPHYLSKIGGVYSSEWFFS
                     KLLHLARTDRKVFESMATFIEICDYIPAVLSGIKNPEEVKRGICAAGHKAIYNEEWGG
                     LPDSEFLEKLDPSFKGIRKKLYEKAYPSGEIAGYLCKEWSKKTGLPEGIPVSVSEFDA
                     HMGAVGAGIKEGVLVKIIGTSSCDMMIYPKNKELKDIPGVCGIVPDSIVPGFYGIEAG
                     QSAVGDIFYWFVKKLIPKFIKDPYSYFTKKAQKIKPGQTGLLALDWHNGNRTILVDQK
                     LTGLIVGLTLNTKPEEIFRCLIESTGFGARVIIERVESFGVKIKEIVASGGIAEKNPL
                     IMQIYADIMNREIKISALPQTCAVGAGIFGAIAGGYFKSVEEAQEKICRYSKKIYRPI
                     PENVIVYEKLYSLYKKLHDAFGTQNYKESLFNVMKELLKIKKETE"
     gene            3022..3690
                     /locus_tag="PLF90_06485"
     CDS             3022..3690
                     /locus_tag="PLF90_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOK56958.1"
                     /translation="MKNLKKRVYEANLFLWKNNLVYGTQGNVSGIDDKREIIYIKPSG
                     VPYEKLTESKIVGVDMNGKLIYGDLKPSVDTPHHLYIYRNLKEVKGFCHTHSKFITVF
                     SVLGIPIPVLTTGHADIFGKDIPVSKYVDNTGDNIGKEILRVYEKTGCPSIILKNHGL
                     FCFGESPEKASFYALMSEYFAEVFYYILIAGKILNKKIKRIPEKEIKKWFERYHSERY
                     GQKK"
     gene            3674..4861
                     /gene="nagA"
                     /locus_tag="PLF90_06490"
     CDS             3674..4861
                     /gene="nagA"
                     /locus_tag="PLF90_06490"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOK56959.1"
                     /translation="MDRKNKFLIKNITLINPGEKGKVVDVYVEGKTIKKIGKDLRKMD
                     VTIEGRGKLLIPGLIDLHIQGAGGTDFLNLKDNENVQKISSTLASLGTTSFLATTIFK
                     PYIKKQKHIERIVENTEKVDGAKILGIHLEGPYINVKRKGMIKEDKICSVRERSIEEI
                     IEICGGKLKMMTFAPEIEGIKSVVKKLIKNNVVPSIGHTDATFDETEEMIKMGVNHVT
                     HLFNAMRPFHHREPGVIGAVLFDNNFTYQIICDGKHIHPEVVNYIFKIKGPENFCIIT
                     DGISALGLPDGNYMYKDIEYFVKEGTGYYKDGTLIGTAFSQLNLMKKIMEFTGLPFEE
                     ILKTATTVPAKVIGISDKKGIIEKGKDADMVILDKNLNVEITFVEGKIVYRKEEKYGN
                     SKI"
     gene            4842..5171
                     /locus_tag="PLF90_06495"
     CDS             4842..5171
                     /locus_tag="PLF90_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56960.1"
                     /translation="MEIVKFEEFKKMDLRVGKIIEVEDVEGADRIYLLTVDIGDEKRK
                     LVAGIKPWYTKEELINKKIVVIVNLEPKIIRGIESKGMLLATMFDNKLSILTTDKEDV
                     PPGSKIT"
     gene            complement(5164..5625)
                     /locus_tag="PLF90_06500"
     CDS             complement(5164..5625)
                     /locus_tag="PLF90_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56961.1"
                     /translation="MRILLYTIVGLALGWWEGVVVVYIREILLNISPDLTKITINQLV
                     KPLFVTHNKYSLLFIEKTREITPIIIILCISIFNEKKFIRKFAAFLWIFAIWDLFYYI
                     TLKILINWPSSLKTIDCLFLIPSPWIAPVWVPVSIMFIFLFISAYIFKNVK"
     gene            complement(5622..6701)
                     /locus_tag="PLF90_06505"
     CDS             complement(5622..6701)
                     /locus_tag="PLF90_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK56962.1"
                     /translation="MTYLERFKETVYFGKPDKPFMWPQWVFGDTVKRWQKEGMPPDVH
                     FNTYFGFDRVEHVPINMGFIPAPEVKVIEEDKETATYIDDLGNKWKRWKNREIGMSQW
                     IEFCIKDEATWENVKKRLNPDSPLRYPQYYDDWKRCVKNRDYPLGVGAGSFYGWVRNW
                     MGVENLSVMFYDNPSLVKEIINYIADFVIRVLEKVVSEVKIDFANIWEDLGMKQGPLL
                     SPKTFEEFCLPAYKKVCSFLRSHGVDVIMVDSDGNNDVIVPLWLEGGVNMLYPLEVAA
                     NTDAVEYRKKYGKKLILMGNIDKRALRNTKKEVEEEVMKKVPFLLKEGGYLPFVDHAV
                     PPDVPLENFKYYLELVGKIWADINK"
     gene            complement(6679..7569)
                     /locus_tag="PLF90_06510"
     CDS             complement(6679..7569)
                     /locus_tag="PLF90_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOK56963.1"
                     /translation="MKEKIKFLESRKKDAHKGDYGHLFIIGGSPGLTGAVCLAGISAL
                     RSGCGLVTIGIPEGLNQIIEIKLTEVMSLPLPETEDHFFSENAYEKAIEFIEKKADCV
                     LIGPGISLHPETKKFGKKIIPDIKKPLIIDADALKIISENLGILKNLRDKPILTPHPG
                     EMSYLTGLKVEQIQRNRVEIAENFAKEYNVILILKGYRTVVTDGENTYINLTGNPGMA
                     TAGSGDVLAGIIGGLLASGEEKFISAKYGVFIHGLAGDMAKREKGEISLIASDIIEKL
                     PDVFKYLKKEEEKNDLFRKI"
     gene            complement(7553..7885)
                     /locus_tag="PLF90_06515"
     CDS             complement(7553..7885)
                     /locus_tag="PLF90_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOK56964.1"
                     /translation="MLNFSIDLESLDEYKEPARVFSFLSEKESNNKSLISLIGKVAAK
                     KAFFKCIGVNENFKKIEIKNSPSGKPYIEIKDEKLKEVLKDKRIEISISHTKNIAVAI
                     CLVYERKD"
     gene            complement(7887..8132)
                     /locus_tag="PLF90_06520"
     CDS             complement(7887..8132)
                     /locus_tag="PLF90_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOK56965.1"
                     /translation="MGRKRQGNGRDSNPKFRGTKVFDGQIVKAGNIIVRQKGSRILPG
                     VGCSIGRDFTIYSLVDGIVEYTQRGDKKVVNVRTVQQ"
     gene            complement(8173..10086)
                     /gene="rlmD"
                     /locus_tag="PLF90_06525"
     CDS             complement(8173..10086)
                     /gene="rlmD"
                     /locus_tag="PLF90_06525"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOK56966.1"
                     /translation="MKIILASKSERRKELLKKICEEFKVVDSNFDESEIKEKDPVKYA
                     IKCAIEKAKIVGEKYSDAVVIGADTVVFLNNEIIGKPSSYDEAKGILKKLSGTEHSVI
                     TGFAIYKKDENKLITDYELSYVKFKDIKDEEIEEYLKTEDFWDKAGAYGIQNIGDRFV
                     EYVKGDFDNVVGLPVKKLKKYLDEFIYEELTVDIYDIALPNNWGVGRYENFVVFVPGG
                     VTGDRVRVRISKSKKNYSFGEIVEIEKSSEYRVKPECEHFGDCGGCVFQNVRYEKQLE
                     IKKEYLISCLKKLGEIEEINLKEIIPSPEIYFYRNKMEFAFGSEDGKLILGLRERSLP
                     YKKYSKRVIPLRRCFIFSKKVEKIFPIVCDFFSSLNISSYDPFKKTGILRHLVIKESK
                     NKDGIMLIFVTKSGVDIDFSKLIGLLLKEIEKIKSIYWVENDQISDVVNYEKKHILYG
                     EKFIEEKINNLTFRIYPETFFQPNTKCAEILYKKILENIDEKSKVLGLYCGTGPIEIT
                     VSKKAEEVIGVDWDISNINTAVENCKINQIKNCNFYAERAEKFLNRELHEFFNYLILD
                     PPRGGLSSKSIKKILKKKIPNIIYISCNPSTLSRDLKEFKEGGYSIEKILLFDFFPHT
                     SHLESFVLLKKYE"
     gene            complement(10083..>10588)
                     /locus_tag="PLF90_06530"
     CDS             complement(10083..>10588)
                     /locus_tag="PLF90_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56967.1"
                     /translation="SGAKHIVSSIMDIPQAIKDDVLIYIKKFFGEDIKRKIEILYNER
                     IGNWFHADIRYRREIFTFLKEVCDKYNISFALCMEYEKKNGKIIGLNREFMTSKNCEG
                     IDIPVYFRKGKKFYPFNCYGNCLKCKTAECGIEELAQGNKEDSFSGWDYYDYLRWNKI
                     VGQEKLL"
BASE COUNT         3630 a         1809 c         1400 g         3749 t
ORIGIN      
        1 catattttca aatccaagca atacttcact tgctatttca aaaacaccac tgcttggaca
       61 gacaagcatc ttcattccat ctggtaaatt ttttgaagca atttcatata aagaataatc
      121 aatttcttct ttcttctgcc agggataatt ttcaaaatca gaccagtttg ttatcattcc
      181 agttccttct tcaacccatc ctctttctcc ctttgataaa attgcagtat ctttccccct
      241 tcttgatttt ccagtaaata taattccgcc tgcaattcgt acataatcat aaccaagttt
      301 ataccagaag tttatttcct gcttaatata tttttcttta ttctcttccg aatattcaat
      361 ccatttttct ccaataattt tttctgttat atattttttc atttcataat ccattaaaag
      421 ttccacaaaa tgaactcttt ctgctttctg atttccaagc aagacatttt ttaattcttc
      481 aaaatctggt tctggttttt ctaaagggac ttttaaattc atatctccct cctcttttta
      541 ataatgaaaa agttcttctt tccaaaaatt aaacggagag ggtgggattt gaacccacgt
      601 ccgccatgtc ctttggcgga agccgatttc gagtcggccg ctttcatcca ctcagccacc
      661 tctccttggt ccaaaaatcc ctgttcctat tctaattata tttgctccct cctctattgc
      721 tattttccac gtatctgtca ttcccattga aagatatttc atttctacat ttggcagatt
      781 taagtttttc aatttttcaa ataaatcctt tgtcaattta aaataaggtc ttatatcttc
      841 tggattttca acaaaaggtc ccatggtcat taatccctct accctcacat tttcaaattt
      901 tgatatttca taaattaatt cttccacctt ctcaggcaaa acacctgact tttgtaattc
      961 cctgccacta tttatctcaa tcaaaactgg ataaattact ttctttttct ttgcttcttt
     1021 atctatttct cttataagtt ttaaggaatc aactgtttca atcatatcaa aaatttcaaa
     1081 tgctttttta accttatttg tctgaagatg tcctatcata tgccatttta ctttttttcc
     1141 gatttttata aatttttttt ctgcttcctg aacataattt tctccaatga atttaatacc
     1201 tccttcaatt gcttcatcta tctcttcaac tgttcttcct tttgttgctg caagaaggat
     1261 tacattttca ggaacttctt ttaaaatttt ctgtactctt tcttttatgc ttacttttga
     1321 attaaccatc tttttattat acaattttaa aaaaggaggg taaatgtttt caatcggaat
     1381 tgatttcgga acaaattcag ttagagggat tattttgaat cttaaaaatg gagaaattgt
     1441 ttctgaaaaa gttgtgccct acccttcagg aattgatgga gtaattattg atgaaaaaga
     1501 cccacatctt gcaagacaga atccggcaga ttatcatatc tgtcttgaaa aacttttaaa
     1561 ggaaattatt aaaaaagcaa aaaaaaataa aagtttttct gttgataaaa ttgttgggat
     1621 aggaatagat acaacaggtt caactccaat gcctgttgat aaaaatcttg ttcccctttc
     1681 tttttataaa gaatttaaaa ataatgttaa tgcaatggca tggttatgga aagaccatac
     1741 ttcaagcgat gaagcagaag aaataactga actttcaaaa aaaatcagac cccattattt
     1801 atcaaaaata ggaggagttt attcatctga atggtttttt tcaaaattac ttcaccttgc
     1861 aagaactgat agaaaagttt ttgaatcaat ggcaacattc attgaaatat gcgattatat
     1921 acctgcggtc ttatcaggaa taaaaaatcc agaagaagta aaaagaggaa tatgtgcagc
     1981 aggtcataaa gcaatatata atgaagaatg gggtggatta ccggacagtg aatttcttga
     2041 aaaacttgac ccttcattta aaggaataag aaaaaaactt tatgaaaagg catatccaag
     2101 tggtgaaatt gcaggttatt tatgtaaaga atggagtaaa aaaacaggtc ttccagaagg
     2161 aattccagta agtgtttctg aatttgatgc tcatatggga gcggtcggag caggaataaa
     2221 agaaggagtt cttgtaaaaa taatcggtac ttcttcctgt gatatgatga tatatccaaa
     2281 aaataaagaa ctaaaagaca taccgggagt ttgtggaatt gtacccgatt ctatagtccc
     2341 aggattttac ggaatagaag cgggtcaatc cgcagtcggt gacatttttt actggtttgt
     2401 aaaaaaattg attccaaaat ttataaaaga cccttattcc tacttcacaa aaaaagcaca
     2461 gaagataaaa cctggtcaaa ctggtcttct tgctcttgac tggcataatg gaaacagaac
     2521 tattcttgtt gaccagaaat taaccggttt aatagttggc ttaactttaa atacaaaacc
     2581 agaggaaatt tttagatgtt taattgaaag tacaggtttt ggagcaagag tgataataga
     2641 aagggtcgaa agttttggag taaaaataaa agagatagtg gcttctggtg gaatagcaga
     2701 aaaaaatcct ttaattatgc agatatatgc cgacataatg aacagagaaa taaaaatatc
     2761 agcacttcct caaacatgtg cagttggagc tgggattttt ggagcaatag caggtggtta
     2821 ttttaaatca gttgaagaag cacaggaaaa aatttgtaga tacagcaaaa aaatttatag
     2881 acccattccg gaaaatgtaa tagtatatga gaaactttat tcactttata aaaaacttca
     2941 tgacgctttt ggaacacaga attataaaga aagtctgttt aatgtaatga aggaattgtt
     3001 aaaaataaaa aaggaaacag aatgaagaat ttaaaaaaaa gggtctatga agcaaatttg
     3061 tttttatgga aaaacaatct tgtttatgga acccagggaa atgtaagtgg aatagatgat
     3121 aaacgagaaa ttatttatat aaaaccaagt ggtgttccgt atgaaaaact tacagaatca
     3181 aaaattgtcg gagttgatat gaatggaaaa ctgatatatg gggatttaaa accatctgtt
     3241 gatacaccac accatcttta catttacaga aatttaaaag aagtcaaagg attctgccat
     3301 actcattcaa aattcataac tgtcttttca gttcttggca ttccaattcc tgtacttaca
     3361 acaggtcatg cagatatttt tggaaaggat atacctgttt caaaatatgt tgataataca
     3421 ggtgataaca taggaaaaga aattttaaga gtatatgaaa aaacaggctg tccatcaata
     3481 attttaaaaa atcatggcct tttctgtttt ggagaatctc cggaaaaagc atccttttat
     3541 gctcttatga gtgaatactt tgcagaagtt ttttattata ttttaatcgc tggaaaaata
     3601 ttgaataaaa aaattaaaag aattcctgaa aaagaaataa aaaaatggtt tgaaagatac
     3661 cattcagaaa gatatggaca gaaaaaataa atttttaatt aaaaatatca ctcttatcaa
     3721 tccaggagag aaagggaaag ttgtggatgt ttatgttgaa gggaaaacca taaaaaaaat
     3781 tggaaaagat ttaagaaaaa tggatgttac aatagaagga aggggcaaac ttttaattcc
     3841 aggattgatt gacctccata ttcaaggtgc tggtggaact gattttctta acctaaaaga
     3901 taatgaaaac gttcaaaaaa tttcgtctac ccttgcttct ttaggaacaa cctcttttct
     3961 tgcaacaaca atttttaagc cctatatcaa aaaacagaag catatcgaaa gaattgttga
     4021 aaatacagaa aaagtagatg gagcaaaaat tctcggaatt catcttgaag gtccttatat
     4081 aaatgttaaa agaaaaggga tgataaaaga agacaaaata tgttctgtaa gggaaaggtc
     4141 aatagaagag ataattgaaa tatgcggtgg aaaacttaaa atgatgactt ttgcaccgga
     4201 aatagagggc ataaaatcag ttgttaaaaa attaatcaaa aataatgttg ttccttctat
     4261 tggacataca gatgcaacat ttgatgaaac agaagaaatg ataaagatgg gagtaaatca
     4321 tgtaacccat ttatttaatg caatgaggcc tttccaccat agagaacctg gagtaattgg
     4381 tgcagttttg tttgataata attttaccta tcagataata tgcgatggaa aacacataca
     4441 tccagaagtt gtaaattata tttttaaaat taagggtcct gaaaatttct gtataataac
     4501 agatggcata agtgctcttg gattacctga cggaaattat atgtataaag acatagagta
     4561 ttttgttaaa gaaggaacag gatattataa agatgggact ttaataggaa ccgctttttc
     4621 acaactgaat ttaatgaaaa aaataatgga atttacaggc ctcccttttg aagaaatttt
     4681 gaaaactgca acaacagttc ctgcaaaggt tatcggaatt tctgataaaa agggaataat
     4741 tgaaaaaggt aaggatgctg atatggttat acttgataaa aatcttaatg tggaaataac
     4801 ttttgtggaa ggtaaaattg tttacagaaa ggaggaaaaa tatggaaata gtaaaatttg
     4861 aagaatttaa aaaaatggat ttaagggttg gaaaaattat tgaagttgaa gatgtggaag
     4921 gagcagatag aatttattta ctaacagttg atatcggaga tgaaaaaagg aaattggttg
     4981 ctggtataaa accatggtac acaaaggaag aattgataaa caaaaaaata gttgttattg
     5041 tcaatcttga accaaaaata atcaggggaa ttgagagtaa aggtatgctt cttgcaacaa
     5101 tgtttgataa taaactttca attctgacaa cagataaaga ggatgttcca ccaggaagta
     5161 aaattactta acatttttaa agatgtaagc agaaataaaa aggaaaataa acattataga
     5221 aacaggaacc cagacaggag caatccaggg agagggaatt aaaaagaggc aatctattgt
     5281 ttttaaagaa gaaggccagt taatcaaaat tttaagtgta atataataaa ataaatccca
     5341 gatggcaaaa atccacaaaa atgctgcaaa ttttctgata aatttttttt cattgaaaat
     5401 tgaaatacat agtattataa tgattggtgt aatttctctt gttttttcaa taaaaagcaa
     5461 actgtattta ttatgggtta caaaaagcgg ttttacaagt tgatttattg taatttttgt
     5521 taaatcaggt gatatattca aaagaatttc tcttatatat actacaacaa ctccttccca
     5581 ccatccaaga gcaagtccaa ctattgtata caaaagaatt ctcatttatt tatatctgcc
     5641 catattttac caacaagttc aagataatat ttaaaatttt caagtggtac atcaggtgga
     5701 actgcatggt caacaaaggg taaatatcct ccttctttca aaagaaatgg tacttttttc
     5761 attacttctt cctctacttc tttctttgta tttctcaatg ctcttttatc tatatttccc
     5821 atcaaaatca atttttttcc atattttttt ctgtattcaa ctgcatcagt atttgctgca
     5881 acttcaagtg gataaagcat atttacacca ccctcaagcc acaaaggaac tattacatcg
     5941 ttatttccgt ctgaatcaac cataattaca tctacaccat gacttcttaa aaatgaacaa
     6001 acctttttat aagcaggaag gcagaattct tcaaatgttt ttggtgaaag taaaggtccc
     6061 tgtttcattc caaggtcctc ccatatattt gcaaaatcaa tttttacttc actcacaact
     6121 ttttccagca ctcttataac aaaatcagca atataattta ttatttcttt aacgagtgaa
     6181 gggttatcat aaaacataac tgataaattt tcaacaccca tccagttcct tacccatcca
     6241 taaaatgaac ctgctcctac tccaagagga taatctctgt tttttacaca tcttttccag
     6301 tcatcataat attgtggata tcttaaagga gaatctggat ttaatctttt tttaacattc
     6361 tcccaagttg cctcatcttt aatacagaat tcaatccact gactcattcc tatttctcta
     6421 tttttccatc ttttccattt attgccaagg tcatctatat aagtggcggt ttccttatct
     6481 tcttctataa cttttacttc tggagcagga ataaaaccca tattaatagg cacatgttcc
     6541 actctatcaa aaccaaaata agtattgaaa tgaacatccg gtggcatacc ttctttttgc
     6601 catcttttaa ctgtatctcc aaaaacccat tgaggccaca taaatggttt atctggcttt
     6661 ccaaaataaa ctgtctcttt aaatctttct aaataagtca tttttttcct cctctttttt
     6721 taaatattta aaaacatctg gtaatttttc tattatatca gatgcaatta aagaaatttc
     6781 tcctttttct ctttttgcca tatctcctgc aagtccatga ataaaaacac catattttgc
     6841 agaaataaat ttttcttccc ctgatgccag aagtcctcct ataattccag caagaacatc
     6901 accacttcct gctgttgcca tccctggatt tccggttaaa tttatatatg tattttcacc
     6961 atctgtaacc acggttctat atcctttcag aattaaaata acattatact cttttgcaaa
     7021 attttctgct atttccactc tatttctctg tatctgttct actttcaatc ctgtaagata
     7081 actcatttct ccgggatgcg gtgttaaaat aggtttatct cttaaatttt ttaaaattcc
     7141 aagattttct gaaataattt ttaaagcatc agcatctatt attaatggtt ttttaatatc
     7201 tggaattatc tttttaccaa atttttttgt ttccggatgt aatgatattc cgggacctat
     7261 taaaacacaa tctgcttttt tttctataaa ctcaattgcc ttttcatatg cattttcaga
     7321 aaaaaaatgg tcttctgttt caggtaatgg caaactcata acttctgtaa gtttaatttc
     7381 tataatttga ttaagtcctt caggtattcc tattgttaca agtccgcatc cacttcttaa
     7441 agcacttatt ccagcaagac agacagcacc tgttaaacca ggactcccac caataataaa
     7501 aagatgtcca taatcccctt tatgtgcatc tttttttcta ctttccagaa atttaatctt
     7561 ttctttcata aaccaaacag atagcaacag caatattttt tgtgtgggaa atagatattt
     7621 ctattctttt atcttttaat acttccttca atttttcatc tttaatctct atatatggct
     7681 tcccagaagg actattttta atctctattt ttttaaagtt ttcattaact ccaatacatt
     7741 taaaaaatgc ttttttggca gcaactttac caatcaaaga tattaaactt ttattattgc
     7801 tttctttttc ggataaaaaa gaaaaaactc tggcaggttc tttatattca tcaagggact
     7861 ctaaatctat tgaaaagttt aacattttat tgctgaactg ttcttacatt aactactttt
     7921 ttatcacctc tttgtgtata ttcaacaatt ccatcaacca aactatatat tgtaaagtcc
     7981 cttccaatag aacatccaac gccaggaaga attctactgc ctttttgtct cacaataata
     8041 ttacctgcct taacaatctg tccatcaaaa acttttgttc ctctaaattt tggatttgaa
     8101 tctctaccgt ttccctgtct ttttcttccc atagtaaaaa attataacac tttttatatt
     8161 tagagtcaat ttttattcat actttttcaa taaaacaaaa gattcaagat gggatgtgtg
     8221 agggaaaaaa tcaaatagaa gtattttttc tatagagtaa cctccttctt taaactcttt
     8281 taaatctctt gataaagttg aaggattaca ggaaatgtaa ataatattgg gtattttttt
     8341 ctttaatatt ttttttatac tttttgaact caatccacct cttggtgggt caagtattaa
     8401 atagttaaaa aattcgtgta attctctatt taaaaatttt tctgctcttt cagcataaaa
     8461 attacagttt tttatctggt ttatcttaca attttcaaca gcagtattaa tatttgaaat
     8521 atcccaatca actcctatta cttcctctgc ttttttagaa acagttattt caattggtcc
     8581 tgttccacag tataaaccaa ggactttact cttttcatca atattttcaa gaattttttt
     8641 atataaaatt tctgcacatt ttgtattcgg ttgaaaaaat gtctcaggat aaattctaaa
     8701 agttaaatta tttatctttt cttcaatgaa tttttctcca tatagaatat gttttttctc
     8761 ataattaaca acatctgata tctggtcatt ttctacccag taaatacttt ttattttttc
     8821 aatttccttc aacagtaaac caatcagttt agaaaaatca atatcaactc cactttttgt
     8881 cacaaaaata agcattatcc catctttatt tttgctttcc ttaataacaa gatgcctcaa
     8941 aatacctgtc tttttaaaag ggtcataaga agagatattc aatgaagaaa aaaaatcaca
     9001 aacaattggg aaaatttttt ctaccttttt actgaaaata aaacatcttc tcaaaggaat
     9061 aactctttta ctatattttt tataaggtaa acttctttct ctcaatccaa gaatcagttt
     9121 tccatcttca ctaccaaatg caaactccat cttatttctg taaaaataaa tttcaggtga
     9181 tggtataatt tctttcaaat taatctcctc aatttctcct aattttttta aacatgaaat
     9241 cagatattct ttttttatct caagttgttt ttcataacga acattctgaa aaacacatcc
     9301 accacaatct ccaaaatgtt cgcattcagg tttaactcta tactctgagg acttttcaat
     9361 ttcaacgatc tctccgaaag agtaattctt ttttgacttt gaaatcctaa ctcttaccct
     9421 gtctccagta actccaccag gaacaaatac aacaaaattt tcataccttc caactcccca
     9481 gttatttgga agggcaatat cataaatatc aaccgttaat tcttcataaa taaattcatc
     9541 aagatatttt tttaattttt ttacaggtaa accaacaaca ttatcaaaat cacctttaac
     9601 atattcaaca aatctatccc caatgttctg aattccataa gcacccgctt tatcccaaaa
     9661 atcctctgtc tttaaatatt cctcaatttc ttcatcctta atatctttaa actttacata
     9721 acttaattca taatcagtaa tcaatttatt ttcatccttt ttatatatcg caaaccctgt
     9781 aattactgaa tgttctgtac cggataattt tttaagaatc ccctttgcct catcataact
     9841 tgacggttta cctataattt cattatttaa aaaaacaaca gtatcagcac ctatgacaac
     9901 tgcatcggaa tatttttctc caacaatttt tgccttttca attgcacatt taattgcata
     9961 tttaacaggg tctttttctt ttatttcact ctcatcaaaa ttactgtcaa caactttaaa
    10021 ttcttcacaa attttcttca ataactcttt tcttctttct gattttgatg caagtataat
    10081 tttcataaaa gtttctcctg tcctacaatc ttattccatc ttaaataatc gtaataatcc
    10141 catcctgaaa aactgtcttc tttatttccc tgtgcaagtt cttctattcc acattcagca
    10201 gttttacatt ttaagcaatt tccgtaacaa ttaaatggat aaaacttttt tccttttctg
    10261 aaataaacag gtatatcaat tccttcacaa tttttacttg tcataaattc cctatttaaa
    10321 cctattattt taccattttt tttctcgtat tccatacata aagcaaaaga aatgttatat
    10381 ttgtcacaaa cttcttttaa aaaagtaaaa atttctcttc tatatctaat atcagcatga
    10441 aaccagttcc ctattctctc attatataaa atttctattt ttctctttat atcctctcca
    10501 aaaaattttt ttatatatat caacacatca tcctttattg cctgtggaat atccataata
    10561 cttgaaacaa tatgttttgc tccactgt
//
LOCUS       DAOSAT010000065        10460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_4211_length_10460_cov_5.133397, whole genome shotgun sequence.
ACCESSION   DAOSAT010000065 DAOSAT010000000
VERSION     DAOSAT010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10460
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4211_length_10460_cov_5.133397"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLF90_06535"
     CDS             complement(<1..575)
                     /locus_tag="PLF90_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56968.1"
                     /translation="MIYAKFKDGLEVSEGIFKVLARFAPDGVMDVFASDQHSSYIKLT
                     NSFLKHIGEKREANDEDVKEVCKRFARHLRGEVTAALFNHLAILTPGFKEALGSEILL
                     IGRLEHTKPVELDGGLGKVARLAIEPEEIADKVDAFKTLVYLNPDHKESWEKNREWLA
                     DVFEKCKKLEKPLYNETLLADLPGETKLEKA"
     gene            complement(591..1043)
                     /locus_tag="PLF90_06540"
     CDS             complement(591..1043)
                     /locus_tag="PLF90_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HOK56969.1"
                     /translation="MAKVIIVVDNENDLKKIDEGKKILEELKIFYDVKIISGMNNLNP
                     VDELVELIEKTEIDVVIVCSGISTHLPGIIASKTIIPVIGVPLPVNDIPSTETIFSIA
                     QMPEGVPVACMSLGKTGIKNAVIFAAQIISRKDENLRKKVKEFKNQYI"
     gene            complement(1031..2188)
                     /locus_tag="PLF90_06545"
     CDS             complement(1031..2188)
                     /locus_tag="PLF90_06545"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOK56970.1"
                     /translation="MFEVSERLRKIPPYLFVEIDRKKKELNAKGESVIDFGVGDPDLP
                     TPSNIIEAMKKAVENPNFHRYPFGKGTKEFRKAIAEYYKKNYDVEIDYENEICVLIGS
                     KEGIAHFPLCFINNGDISLVPEPGYPVYHLGTLIAGGESYFLPLKKENEFLPELGKVP
                     EEIIKKTKILYLNYPNNPTGSFACYDFLKEAINFCKKNNIILVYDAAYSEIYFEKKPV
                     NIFSIDGAKDVVIEFNSLSKTYNMTGWRIGWACGNTYLISALAKIKENIDSGTFEAIQ
                     FAGIEALTGSQESVIKIREIYKRRRDIFATGLKELGFDFNLPSGTFYFWVKINESSIK
                     FVDFLLENGKIVATPGVGFGPSGEGYIRFSLTINEEKIKEAISRMKKIWQK"
     gene            complement(2181..2978)
                     /gene="dapB"
                     /locus_tag="PLF90_06550"
     CDS             complement(2181..2978)
                     /gene="dapB"
                     /locus_tag="PLF90_06550"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOK56971.1"
                     /translation="MEKINLIVCGACGRMGSLIIELALKDKDFFVKGGIETKSHPMIG
                     YEIKEGIKVFDSLDEIVEKDDIVIDFTVPSATMSFIKTVKEKRGKIVIGTTGFKSEEI
                     AKIKEISEYIPVFLSPNMSLGVNLFFEVIKYATKLFKNYEIEIEEIHHHFKKDAPSGT
                     AKKIAEIICNERNLKIEKILKYGRSGETGERQFDEIGIHSLRIGDIVGEHTVFFGGTG
                     EIIEISHRCYNREAFASGALKVSKYLNKKDKGLYGMENFIKEEVLNV"
     gene            complement(2981..4246)
                     /gene="murA"
                     /locus_tag="PLF90_06555"
     CDS             complement(2981..4246)
                     /gene="murA"
                     /locus_tag="PLF90_06555"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOK56972.1"
                     /translation="MEKFIIEGGNKLEGEIEVSGSKNASLPIIAASIMGEGKTVLYNV
                     PDLKDIQTMIKILNYMGVNTKFENNTLEIDPSGIYQFTAPYEFVSTMRGSICLLGPLL
                     AKYKKARFSMPGGCVIGPRPIDLHIKGLKKLGVQIENEEGYIVGKCDNLKGTKVFLGG
                     NFGSSVLATANVMCAAVLAEGETIIEFAACEPEIVDLANFLKKMGARIYGEGSHIIRI
                     EGVKKLKGVEYKIIPDRIEGGTYILTGYITKSKIKINGVITEHLLSLFDKIEESGLEI
                     YYGDNFVVTIPKERWKAVEITTLPFPGFPTDLQAQMMSFLTLADGISLITEKVFPERF
                     IHVGELNRLGADITLDGSKAIIKGVELLKGTKVMASDLRASAALVLAGLVADGTTEIS
                     RIYHLDRGYEKFEKKLNMLGAKIKRVREE"
     gene            complement(4282..5124)
                     /gene="prmC"
                     /locus_tag="PLF90_06560"
     CDS             complement(4282..5124)
                     /gene="prmC"
                     /locus_tag="PLF90_06560"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOK56973.1"
                     /translation="MDALTFLKKTEKELQKKGISSPRIELEILLSHILGIERYKIYIE
                     KIFLSEKIQKKLLKLIEERKKRIPTAYLTKKIYFYDCEFIIEKGVFIPRPETELLVEK
                     TINLYKEYFYPKKVKILDIGTGCGNIAITLAKNIDNCLVTGIDFSKKSLKIAHRNVFL
                     NNVEKKVNLLFSNIFSEIKEKFEIIVSNPPYVSENEYKKLEKEITKEPKRALIGGKDG
                     LKIIEKILKGSLYHLKKNGFLIIEIGYNQAPKIRKFVPPELKLFSIEKDFSSFERIMV
                     FKKI"
     gene            complement(5134..6972)
                     /locus_tag="PLF90_06565"
     CDS             complement(5134..6972)
                     /locus_tag="PLF90_06565"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK56974.1"
                     /translation="MKKNIILMFTIFTFLVYGREFTDLINEGVIYLKTEEYVKAINIF
                     EEAKKISPENSDVYYYLGEAYYRKGESDKAITNLQKAININSQNLSYYYTLALVYLSQ
                     NKKSEAIETLNKIINTAPLTYYGKNAIKLKNDIENSEKEIQITKKWEQQEIEEKKRKE
                     EEKKKEEKTTSEGLPSNILQELPPEMLKNIGTTQSSEVTKTPIPTLIKRIKFGTEEIR
                     KKASGEIVTYSATEIQKVISDILSIIKDEKEPEVKRNLILAAGKINNSEVIDMLLEII
                     KDEEELFEIRIVALDTISNLKAEKVIEELRNTLSSMISRREKEREDARKNIQEINTKV
                     DDLTAKQIVLNSEIQNLNNRIAQIDNQLYSYNEEFPAGAPPGITPGGRKPLTDKETKK
                     LLEEKRISEEKIKTNNEEIEKIKKELEDLTTRKRKYEELLELRKRKIDITGIGASAAV
                     IQEETPEQPYFGFASPDFSSYQRETNEEKNEIIFAVKLMNALANLRDTDSLPVIKKAW
                     REFGVPGLRMYYYIALGKLGEYRSIDLMVSRLKENYPQGNREEEIYIRASIIEVLGEY
                     LKQNPDNEIKGLIEYLAEEGEYPLIKNTAKKVISSLVKDEVKEAKN"
     gene            complement(7040..8080)
                     /gene="prfA"
                     /locus_tag="PLF90_06570"
     CDS             complement(7040..8080)
                     /gene="prfA"
                     /locus_tag="PLF90_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOK56975.1"
                     /translation="MVDEKIKEIEIEYRQYLELLSQEKKDTEKFKEVLKKISVYEEIL
                     KLKKKYDEILKKIEEDKAIIENGEEELQKIAKEELDSLIKEKNKIEIEIENLLNPENE
                     VNNKNAIVEIRAGIGGEEACLFVGDLFRMYLKYCEKNNFKIEILDSHPSELGGYKEII
                     FSVKGKGAYGNLKYEGGVHRVQRVPKTESYGRIHTSAATVAVLPEIESEEIKINPEDL
                     KIETFRASGHGGQHVNKTSSAVRITHIPSGITVSCQDDRSQLKNREKAMKILRARLQD
                     IYEREKKEKIDTERKKLIKTGDRSEKIRTYNFPQNRLTDHRINFTSYNLDKIMDGELD
                     ELIEQLKKQMKE"
     gene            complement(8070..8267)
                     /gene="rpmE"
                     /locus_tag="PLF90_06575"
     CDS             complement(8070..8267)
                     /gene="rpmE"
                     /locus_tag="PLF90_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOK56976.1"
                     /translation="MKKGIHPEYKDATIVCACGNIVKTRSTKSEIKVEICNKCHPYFT
                     GKQKFVDSTGRVEKFKKKYGG"
     gene            complement(8245..8829)
                     /locus_tag="PLF90_06580"
     CDS             complement(8245..8829)
                     /locus_tag="PLF90_06580"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOK56977.1"
                     /translation="MEKKEKIVIYVLFSIFILLLLLLGTWEIIEKFTQKTNVYIFLVK
                     QEKDKTIVKPVIRKIKYYRGIENKIENTMRELIKGPTEEEKKNGFSTSLNENTRILDL
                     KIEGDTLNLNFSKEVEEGGGTLLMETRIAQIVYTGTQFPEIEKIRFLIEGNTIKYFSG
                     EGITIVEKPVSRDDLKEFEIEIIEEGKNEKRNSS"
     gene            complement(8919..10229)
                     /locus_tag="PLF90_06585"
     CDS             complement(8919..10229)
                     /locus_tag="PLF90_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOK56978.1"
                     /translation="MVEINNLVKKIEKKEIKVGVIGLGYVGLPLVIRFIESGFNVIGF
                     DTDEEKVVKLKKGISYIHYIPEEKIKQIIPNLEPTTDMRRLKEVDAIVICVPTPLNPY
                     REPDLTYLHKTADDIYENLREGHIISLESTTYPGTTREIYLSKFEKKGLKAGEDFFLI
                     YSPEREDPGNPKYKTKDIPKVVGGITENCKKIGIVLYSQIVDEVVPVSSTEVAEATKL
                     LENIYRAVNIALVNELKMLFEKMGIDIWEVIEASKTKPFGFQPFYPGPGLGGHCIPID
                     PFYLTWVAKRYDFTTSFIELAGEINTMMPYYVVEKCIEGLNMQGKPIKGSKILIVGIA
                     YKKDVDDIRESPAFKIIKILEKKNVDRIDYYDPFVKEVKKTRQYPKPIFSIEFSPEKL
                     KNYDVAIIVTDHSNIDYKTLYDCVPLIIDTRNVYKEKLPKVIKA"
     gene            complement(10254..>10460)
                     /locus_tag="PLF90_06590"
     CDS             complement(10254..>10460)
                     /locus_tag="PLF90_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56979.1"
                     /translation="IYEGIIDKVFIEKGKIKIYEFKTYFSDLEKYKEQLEIYKIAMKK
                     IFNINNVECFIINLSEVKIVKLEE"
BASE COUNT         3012 a         1837 c         1168 g         4443 t
ORIGIN      
        1 tttgcttttt caagttttgt ctcacccggt aaatctgcaa gtaatgtttc attataaaga
       61 ggtttttcaa gttttttaca tttttcaaag acatctgcaa gccattctct gtttttttcc
      121 caactttctt tatggtcggg atttagatac acaagtgttt tgaatgcatc tactttgtct
      181 gcaatttctt ctggttctat tgcaagacgg gcaactttac ctaatcctcc atcaagttca
      241 actggttttg tatgttcaag tcgtccaatt aaaagtattt ctgaaccaag agcttcttta
      301 aatcccggtg ttaaaatagc aagatgatta aataatgcag ctgtaacttc gccccttaga
      361 tgtcttgcaa atcttttaca tacttccttg acatcttcat cattggcttc tcttttttca
      421 cctatgtgtt ttaaaaaact atttgtaagt ttaatatagg aactatgctg atccgaagca
      481 aaaacatcca taacaccatc tggagcaaac cttgcaagca ctttaaaaat accctcactt
      541 acttccaaac catccttaaa tttagcatat atcatcattt acctcctttt ttaaatatat
      601 tggtttttaa attcttttac tttttttctt aaattttcat cttttcttga tataatctgg
      661 gctgcaaaga taactgcatt ttttatacct gttttcccca gagacataca ggcaactgga
      721 actccctcag gcatctgtgc aattgaaaat atggtttctg tactgggaat atcattaaca
      781 ggtaaaggta caccaataac cgggatgatt gttttggaag caataatacc aggtaaatga
      841 gttgaaattc cactgcagac aattactaca tctatctctg ttttttctat taattccaca
      901 agttcatcca ctggatttaa attattcatt ccggaaatta ttttaacatc ataaaaaatt
      961 tttaattctt caaggatttt cttcccttca tcaatttttt ttaagtcatt ttcattatct
     1021 actacaataa ttacttttgc catatctttt tcattcttga aatagcttct tttatttttt
     1081 cttcatttat tgttaaagaa aaacgaatat atccttcacc ggaaggacca aaaccgactc
     1141 ccggagttgc aactatttta ccattttcaa gtaaaaagtc aacaaattta attgaacttt
     1201 catttatttt cacccagaaa taaaatgttc ctgatggtaa attaaaatca aatcccagtt
     1261 ctttcagccc tgttgcaaaa atatctcttc ttcttttgta tatttctcta attttaataa
     1321 ctgattcttg agaacctgta agtgcttcaa ttcctgcgaa ttgaattgct tcaaaggttc
     1381 cagagtcaat gttttcttta attttagcaa gagccgatat taaataagta ttgccacaag
     1441 cccatcctat tctccatcct gtcatattat aagttttgga caatgagtta aattcaatca
     1501 ctacatcttt tgcaccatct attgaaaata tattaactgg ttttttttca aaatatatct
     1561 ctgaataagc agcatcataa acaagaataa tattattttt tttacagaaa tttattgctt
     1621 cttttaaaaa atcataacag gcaaaacttc ctgttgggtt attcggataa tttaaatata
     1681 aaattttcgt ctttttaatt atctcttcgg gaacttttcc cagttccggt aagaattcat
     1741 tttctttttt cagaggtaga aaataacttt cccctcctgc aatcagagtc ccaagatggt
     1801 atacaggata tcctggctct ggaacaagtg aaatatctcc attatttatg aaacacagag
     1861 gaaaatgtgc aattccttct tttgaaccaa tcaaaacaca tatttcattt tcataatcaa
     1921 tttcaacatc atagtttttt ttataatatt cagcaattgc ttttctgaat tcttttgtac
     1981 cttttccaaa aggatatcta tgaaaatttg gattttcaac tgcttttttc attgcttcaa
     2041 ttatatttga aggagtaggt aagtcagggt ctccaactcc aaaatcaatt acagattctc
     2101 cttttgcatt taattctttt ttctttctat ctatttctac aaaaaggtat ggcgggattt
     2161 ttcttaatct ttcacttact tcaaacattt agaacctcct cttttataaa gttttccatt
     2221 ccataaagtc ccttatcttt tttattcaaa tattttgaaa cctttaatgc tcctgatgca
     2281 aatgcttctc tgttatagca tctatgagaa atttcaatta tttcaccagt tcctccaaaa
     2341 aaaacagtat gttcacccac tatatctcca attcttaaag aatgtatacc tatttcatca
     2401 aattgtcttt ctcctgtttc tccacttctt ccatatttca aaattttctc tatttttaaa
     2461 tttctttcat tgcatattat ttctgcaatt ttttttgcag taccacttgg cgcatctttt
     2521 ttaaaatgat gatgtatttc ttctatttca atttcgtaat ttttgaaaag tttagttgca
     2581 tattttataa cttcaaaaaa aagatttaca cctaaactca tatttggtga taaaaaaacc
     2641 ggtatatatt ctgaaatttc ttttattttt gcaatttctt cacttttgaa accagttgtt
     2701 ccaataacta ttttacctct tttttcttta actgtcttta taaaactcat agttgctgaa
     2761 ggtactgtaa aatcaattac tatatcatct ttttctacta tttcatctaa actatcaaaa
     2821 actttaatgc cctctttgat ttcataacca atcattggat ggcttttggt ttcaattcca
     2881 ccttttacaa aaaaatcttt atcttttaga gcaagttcaa ttattaaact tcccattctt
     2941 ccacaggcac cacatacaat cagattaatt ttttccattt ttattcctcc cttacccttt
     3001 taattttagc acccagcata tttaattttt tttcaaattt ttcataccct ctatcaaggt
     3061 gatatattct tgaaatttca gtagttccat cagcgacaag accggcaaga acaagtgctg
     3121 cacttgctct taaatcagaa gccatgactt ttgtgccttt taataactct actcctttta
     3181 ttatcgcttt tgaaccatca agggtaatat ctgcaccaag tcgattcaat tctccaacat
     3241 gaataaatct ttcaggaaaa actttttctg ttatcaaact tattccatct gcaagtgtaa
     3301 gaaaactcat catttgtgct tgtaggtcag taggaaaacc tggaaatgga agggttgtaa
     3361 tttcaactgc tttccatctt tctttgggta tagtgacaac aaaattatct ccataataaa
     3421 tttcaagacc cgattcttct attttatcaa aaagagaaag aagatgttct gttataactc
     3481 cattaatttt tattttactt tttgtaatat atccagtaag tatataagtt cctccttcaa
     3541 ttctatccgg aataatttta tattcaacac cttttaattt cttgactcct tcaattctga
     3601 taatatggga accttctcca tatattctgg ctcccatttt ttttaaaaaa tttgcaaggt
     3661 ctactatttc tggttcacag gcagcaaatt caataattgt ttctccttca gctaaaacag
     3721 cagcacacat cacatttgcg gtagcaagaa cacttgaacc aaaatttcct ccaaggaaaa
     3781 ctttagtacc tttaagattg tcacattttc ctactatata tccttcttca ttttctattt
     3841 gaactccaag tttttttagt ccttttatgt gtaagtcaat tggtcttggt cctattacac
     3901 atccacccgg cattgaaaat cttgcctttt tatattttgc aagtaaaggt cctaaaaggc
     3961 atattgaacc tctcattgtg ctcacgaatt cataaggagc ggtaaattga taaatccctg
     4021 atgggtcaat ttcaagagta ttattttcaa attttgtatt gacacccata tagtttaaaa
     4081 tttttatcat tgtttggata tcttttaaat cagggacatt atataaaaca gtttttcctt
     4141 cacccattat actggcggca attataggta gtgaagcatt ctttgaaccg gatacttcta
     4201 tttctccttc aagtttatta ccaccttcaa taataaattt ttccatatgt ctcctttttt
     4261 gtcatattat tttactatat tttatatttt tttaaagacc ataattcttt caaaagatga
     4321 aaagtccttt tcaatagaaa aaagtttaag ttcaggtgga acaaattttc ttattttcgg
     4381 ggcttgatta taacctattt caattattaa aaacccattt tttttaaggt gatacaaact
     4441 tccttttaaa attttttcta ttattttaag accgtctttt cctccgataa gtgccctttt
     4501 tggttccttt gttatttctt tttccagttt tttatattca ttttcactca catatggtgg
     4561 attacttact attatttcaa atttctcttt tatttcacta aaaatatttg aaaataagag
     4621 attcaccttt ttctcaacat tatttaaaaa aacatttctg tgagcaattt ttaaactttt
     4681 tttagaaaag tcaattcctg taaccagaca attatcaata tttttagcaa gtgtaatagc
     4741 gatgtttcca catccggttc caatgtcaag aattttaact ttttttggat aaaaatactc
     4801 tttatataaa ttgattgttt tttctacaag caattcagtt tctggtcttg gtataaaaac
     4861 tcctttttca attataaatt cacagtcata aaaataaatt tttttagtaa gatatgcagt
     4921 cggtattctt ttttttcttt cttcaattaa ttttaataat tttttctgta ttttttcaga
     4981 taaaaatatt ttttctatat aaattttgta tctttctatt ccaagaatat gtgacaataa
     5041 tatttccagt tctatacgag gtgaacttat gccctttttt tgaagttctt tttctgtttt
     5101 ttttaggaag gttaaagcat ccacagtttt ttattaattt tttgcctctt ttacttcatc
     5161 ttttactaaa gaagatataa cttttttagc cgtattttta atcagtggat attctccttc
     5221 ttctgcaaga tactcaataa gacctttaat ttcattatcg ggattttgct ttaaatattc
     5281 tccaagaact tcaataatac ttgctcttat ataaatttct tcttcgcgat taccctgagg
     5341 atagttttct tttaatctgg ataccattaa atcaatactt ctatattctc caagttttcc
     5401 aagagcaatg taatagtaca ttcttaaacc cggaactcca aattctctcc atgctttttt
     5461 tatgactggt aaagaatctg tatctcttaa atttgcaaga gcattcatta atttaacagc
     5521 aaaaataatt tcattctttt cttcatttgt ttctctttga taagaggaaa aatctggtga
     5581 agcaaatcca aagtaaggtt gttcaggggt ttcttcctgt attactgcag cagatgctcc
     5641 aattccagta atatctattt tcctttttct taattcaagc agttcttcat attttctttt
     5701 tcttgtagtt aaatcttcaa gttctttttt aattttttca atttcctcat tatttgtttt
     5761 gattttttct tcacttatcc ttttttcttc aagtaacttt tttgtttctt tatcagttaa
     5821 aggtttcctt ccaccaggag taattcctgg aggagcacct gcagggaatt cttcattgta
     5881 actgtaaagt tggttatcta tttgagcgat tctattattc aaattctgta tttcgctatt
     5941 caaaacaatt tgttttgctg ttaaatcatc tacctttgta tttatttcct gaatattttt
     6001 tcttgcatct tctctttctt tttctcttct acttatcata cttgaaagtg tattcctcaa
     6061 ttcttcaata actttttctg cctttaaatt actaatagta tcaagagcaa caatccttat
     6121 ttcaaataat tcttcttcat ctttaataat ttcaagtaac atatctatta cttctgaatt
     6181 attaattttt ccagcagcaa gtattaaatt tcttttaact tcaggttctt tttcatcttt
     6241 tataattgaa agaatgtcac ttataacttt ttgtatttca gttgcagaat atgtaactat
     6301 ttcacctgat gccttttttc ttatttcttc agttccaaat tttattcttt ttattaatgt
     6361 agggattggt gtttttgtaa cttcagaaga ttgagttgtt ccaatgttct ttaacatttc
     6421 aggtggtaat tcttgcagta tatttgatgg taatccttcc gatgttgttt tttcttcttt
     6481 ctttttctct tcttcttttc tctttttttc ttctatttct tgctgttccc attttttagt
     6541 aatttgaatt tctttttcac tattttcaat gtcgtttttc agttttattg catttttacc
     6601 gtaatatgtt aaaggtgcgg tatttataat tttgttcaat gtttctatcg cttcactttt
     6661 tttattttgg gataaatata ctaaagcaag tgtatagtaa taagaaagat tttgagaatt
     6721 tatatttatt gctttttgta aatttgtaat cgctttatca gattctccct ttctataata
     6781 tgcttctcca aggtaataat aaacatcgga attctctgga gaaatttttt ttgcttcttc
     6841 aaaaatattt attgctttta catattcttc agtttttaaa tatataacac cctcattgat
     6901 taaatctgta aattctctac cgtatactaa aaaggtgaat attgtaaaca ttaatattat
     6961 atttttcttc atcttttccc tcctttttta tttaatatta ccttaaaaat aaaaggaatg
     7021 tcaattggta attttttcat cactctttca tttgtttttt taactgttca attagttcat
     7081 ctaattcgcc atccataatt ttatcaagat tataacttgt aaaatttatt ctgtggtctg
     7141 ttaatctgtt ttgagggaaa ttataagttc ttattttctc acttctatca cctgttttta
     7201 taagtttttt tctttctgta tctatttttt cctttttttc tctttcataa atatcttgta
     7261 atcttgctct taaaatcttc attgcttttt ctctattttt taattgagat ctatcatcct
     7321 gacaacttac tgtaattccg ctggggatat gggttattct tactgctgaa gatgtcttat
     7381 taacatgttg tccaccatgt ccacttgctc taaatgtttc aatttttaaa tcttctggat
     7441 taattttaat ttcttcgctt tctatttctg gtaagacagc aacagttgct gcggatgtat
     7501 gaattcttcc gtaactttct gtttttggta ctctttgaac tctatgtact ccaccttcat
     7561 attttaaatt accataagct ccttttcctt taacagaaaa aattatttct ttatatcctc
     7621 caagttcaga agggtgagaa tctaaaattt ctattttaaa attgtttttt tcacaatatt
     7681 ttagatacat tctgaagaga tcaccaacaa aaagacatgc ctcttctcca cctattcctg
     7741 ctcttatttc aacaatagca tttttgttat tgacctcatt ttcaggattt aaaagatttt
     7801 ctatttcaat ttctatttta tttttttctt taattaaaga atcaagttct tcttttgcaa
     7861 tcttttgcag ttcttcttcc ccattctcta ttattgcttt atcttcctct atttttttta
     7921 aaatttcatc atatttcttt ttgagtttta atatttcttc ataaacactt atttttttaa
     7981 gaacttcttt gaatttttca gtatctttct tttcctgcga aagaagttca agatattgtc
     8041 tatattctat ttctatttct ttgatttttt catccaccat attttttctt aaatttttct
     8101 actcttccag tgctatcaac gaatttttgt tttcctgtaa aataggggtg gcatttatta
     8161 catatttcca ctttaatttc tgatttcgtt gatcttgttt ttacaatatt tccacaagca
     8221 caaactattg ttgcatcttt atactcagga tgaattcctt ttttcatttt ttccctcctc
     8281 tattatttct atttcaaatt cttttaaatc atctctactg acgggttttt caactattgt
     8341 tattccctca ccgctaaaat atttaatagt atttccttca atcaaaaatc ttattttttc
     8401 tatttcagga aactgtgttc cagtataaac aatctgagca attcttgttt ccattaataa
     8461 agttcctcct ccttcttcaa cctcttttga aaaatttaaa tttaaagtat caccttctat
     8521 ttttaaatcc aaaattcttg tattttcgtt taaacttgta gaaaaaccat tttttttctc
     8581 ttcttctgtg ggtcctttta ttaattcccg catagtattt tctattttgt tttctatccc
     8641 tctataatac ttaatttttc ttatcactgg tttaacaata gttttatcct tttcctgttt
     8701 aaccaaaaaa atgtatacat ttgttttttg tgtaaatttt tcaattattt cccaagtccc
     8761 cagtaaaaga agtaataaaa taaatattga aaacagaaca taaattacaa tcttttcttt
     8821 tttttccata gaaatctttc caaatcaaaa aataataaat acgttttaaa ttcatattat
     8881 acctttttat tttttaaagt caatttaata gaggtttgtt atgcttttat aacttttgga
     8941 agtttttctt tataaacatt tcttgtgtca ataattagag gaacacaatc ataaagtgtt
     9001 ttataatcaa tatttgaatg gtcagtaacg atgattgcaa catcgtaatt ttttaatttt
     9061 tcaggagaaa attcaataga aaaaataggt ttaggatatt gtctcgtttt ttttacctct
     9121 ttcacaaagg ggtcataata atcaattcta tcaacatttt ttttctcaag aatttttata
     9181 attttgaaag caggtgactc tctgatatca tctacatctt ttttgtaagc aatacctact
     9241 attaaaattt tacttccttt tattggtttt ccttgcatat tcagaccttc tatacatttt
     9301 tcaactacat aatagggcat catagtattt atttctcctg ccagttcaat aaaacttgtt
     9361 gtaaaatcat atctttttgc tacccatgtt aaataaaatg ggtcaatggg tatacaatgt
     9421 ccaccaagac ccggtcctgg ataaaacggt tgaaaaccga atggttttgt tttagatgct
     9481 tcaattactt cccatatatc aattcccatt ttttcaaaaa gcattttaag ttcattaaca
     9541 agggctatgt taactgctct ataaatattt tcaagaagtt ttgtcgcttc tgctacttcg
     9601 gtagaagaaa caggtaccac ttcatcaact atttgagaat ataaaactat acctattttt
     9661 ttgcaatttt ctgtaattcc tccaacaacc tttggaatgt cttttgtttt atattttgga
     9721 ttgccagggt cttctctttc tggtgaatat ataagaaaaa aatcttcacc tgctttaagt
     9781 cctttttttt caaattttga aaggtaaatt tctcttgttg ttcctggata agtagttgat
     9841 tcaagtgaaa ttatatgtcc ttctctcaaa ttttcatata tatcatctgc tgttttgtga
     9901 agatatgtca ggtcaggttc tctgtaagga tttagtggtg ttgggacaca tataacaatt
     9961 gcatcaactt cttttaatct tctcatatct gttgttggtt ccagatttgg aataatttgt
    10021 tttatttttt cttcaggaat gtagtgaata taagaaatcc cttttttcaa ttttactact
    10081 ttttcttcat ctgtatcaaa accaataaca ttaaatccac tctctataaa tctaataaca
    10141 aggggtaatc ccacatatcc aagcccaatt acaccaactt ttatttcttt cttttcaatc
    10201 ttttttacta aattattaat ttctaccatg gttaaagttt aacttttaaa tcattattct
    10261 tcaagtttta ctatttttac ctctgaaaga ttaattatga aacactcaac attatttata
    10321 ttgaagattt ttttcatcgc tattttataa atttcaagtt gttctttata tttttctaaa
    10381 tcagagaaat atgttttgaa ttcgtaaatt tttattttcc ctttttctat aaaaacttta
    10441 tcaataattc cttcgtaaat
//
LOCUS       DAOSAT010000066        10211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_4316_length_10211_cov_4.567349, whole genome shotgun sequence.
ACCESSION   DAOSAT010000066 DAOSAT010000000
VERSION     DAOSAT010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10211
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4316_length_10211_cov_4.567349"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLF90_06595"
     CDS             complement(<1..459)
                     /locus_tag="PLF90_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOK56980.1"
                     /translation="MSEKLAIFGGEKLIPEGMIKKWPPIDESDINYVLSSLKGEIHSF
                     GPNCKEFEKEFAEWNGNKFAITTNSGTAALHMGIAGCELGCGDEVIVPAYSWSSSATC
                     VLHHNCIPVFVDIDFMTMNIDVNKIEEKITEKTKGIIVVHLHGLPVEMDKV"
     gene            complement(452..1276)
                     /locus_tag="PLF90_06600"
     CDS             complement(452..1276)
                     /locus_tag="PLF90_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK56981.1"
                     /translation="MKISMMSYTMYRGDWKHDFSIKKLCEFTKNLGLDGIDWVTTYEN
                     KPEEVRKIMDDFGLKTVCYTFFVDINFKEKKLRDKGIEEIKKGFEIAAILGTDKIMLP
                     LRGKKEFSREESRRNVIEGLKEVIKESEKFKIKITVEHFPDINGPFLVSDDINFAIKE
                     IPDLRVTFDSGNVIIGGEKPEEAFINNQKYIIHTHFKDWIISEDGKLSGLDGKKYKPA
                     LVGEGIIDYRKLIKVMKSFNYDGYINLEYEENDYEPDVAMEKGLNFLKSIMEEENE"
     gene            1366..1611
                     /locus_tag="PLF90_06605"
     CDS             1366..1611
                     /locus_tag="PLF90_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56982.1"
                     /translation="MATIYKDRIEGLYCKCTKGDIAIFKILEDKIKCIYCGNEIKCNP
                     PVDFIWKEKCHHCDEKNSTFIIDSTGIKYCSRCGNSK"
     gene            complement(1604..2230)
                     /locus_tag="PLF90_06610"
     CDS             complement(1604..2230)
                     /locus_tag="PLF90_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOK56983.1"
                     /translation="MPLYLIFFTSFLIGFSGAISPGPLLAVTIEETIKRDFKAGPLII
                     IGHSLLELLMVLLLIFGFGNFLKKEMIQLYLSIFGGSFLIYTGFLSFFSSQKIKIEQN
                     INISYKSNYSLIFEGIFVSLSNPYWTIWWITIGLVYLSFAFPYGFKGIFLFFTGHIFS
                     DFLWYSFVSYFIFKSKKLISEKYLKYISSFFSVFLILFGGFLIIRKLI"
     gene            complement(2233..2982)
                     /locus_tag="PLF90_06615"
     CDS             complement(2233..2982)
                     /locus_tag="PLF90_06615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOK56984.1"
                     /translation="MRRIYIGEDCVKIKCNFLIKGGTYHYLKNVLRFETGDRFIGFDG
                     SGREYEIEIKEMKRDEIIGKIIEEKEIYNPELLFNLQLFQCLPKGDKFEFIVREVTQL
                     GVKRIVPVINKRTVVKIPKEKIENKIKRWLKIAEESSKVSGRTFIPEIEKPNKFEECI
                     KEEKDLGIIFWEGEKEVTIKSIIEKIKGIDIKNKKINVFIGPEGGFDEEEIEIARGYG
                     YFTVSLGRRILKVETASVVSIAILIYELENL"
     gene            complement(2979..3779)
                     /locus_tag="PLF90_06620"
     CDS             complement(2979..3779)
                     /locus_tag="PLF90_06620"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOK56985.1"
                     /translation="MNEIEEFLGYIKNSKYIVAFTGAGISTESGIPQFRGQTGLWEKY
                     KPEIYGTLPGLVTQFLISPSKVASFIYDFTYPILNAKPNISHITLSELEKKGYLKAVI
                     TQNIDNLHQEAGSRNVIELHGNIYKWKCKKCGKKEDIGKENLKEFVEKIKIIEGRRNL
                     IKYISDFTRCNCGERKRPDIVFFGELLPEDEFKKAEEESKKADLFIIIGTSGFVKPAS
                     DLPYIAKKNGAKIVEINTEKSYFENIADLKFYQPSGEIFKYVSERFLK"
     gene            complement(3772..4896)
                     /locus_tag="PLF90_06625"
     CDS             complement(3772..4896)
                     /locus_tag="PLF90_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOK56986.1"
                     /translation="MKKSYHSICRWTFNPGKGGFVPSNIRPSWSGDKFNSVDFIKIVA
                     EKIKPRMPENVILGVEFHYDSEVNEKNVDEVVGIMKEKNIYLALITPGAHSHFAYGGI
                     ASLDTDEKKKAEEFGIKTVDIAYEKMKDVFLKGKEPTFVLWNGSWGYDIATPFIKKMY
                     DNLKESIAKLCKYEEKKGGKLYFAIEPKPNEGHPAMLVPTVASAILFWKKLKEEYGIT
                     VEKKGVNKEFGHSEMIGLDHIYDTVEEIDNKMLIHMHINSQGYNDGIILGGPGKFDID
                     HGARINGMNIVIAKLIEDAGFDRWKGHDMQARPYDNEEKAIERVIRSILSWEACEKVT
                     EKMNTDEILKYLSNRETDKVEDMIKDLVTEGFKVFKELYE"
     gene            complement(4917..6413)
                     /gene="gndA"
                     /locus_tag="PLF90_06630"
     CDS             complement(4917..6413)
                     /gene="gndA"
                     /locus_tag="PLF90_06630"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HOK56987.1"
                     /translation="MKKAEIGIIGMEVMGRNLALNFERNGYTVCVFNRTGEKTKAFVE
                     TQGKGKNIIPTYDISDFVTNLERPRKIMLMVKEGQPVDDFISKLLPLLEKGDLIIDGG
                     NSFFKDTIRRNKELSEKGILFIGTGVSGGEEGALKGPAIMPGGQFEAYKLVEEMLKKI
                     SAKAYDGEPCVTYIGPDGAGHFVKMVHNGIEYGDMQLIGECCWFFKNGFGMTSGEIAD
                     IMIEWNGENDILRSYLIQITGDLMKEKHKVKPDEYLVDIVADITRMKGTGTWTVQSAL
                     ELLVPVPTIASAVFSREMSQDKDIRLNVSSLLKLDIKEKFKGSKKNIIKIAHDALYIA
                     KISSYAQGLALLKAASEFYKWDLKLGEIAKIWRAGCIIRAQFLDEITKAYKENPDLPN
                     LLVDKNFSEFITKNIKKLAKFIEISHKAGVPVPAFSNSYDYILQITSPVMFSAQVSAL
                     QRDYFGAHGYFKLKGIDNPEILKTEDGKEGEFHTEWLVEGRPEKEVTK"
     gene            6572..6886
                     /locus_tag="PLF90_06635"
     CDS             6572..6886
                     /locus_tag="PLF90_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56988.1"
                     /translation="MNKKINKTVKIDIKEGLKKFYPEIFEDGKVNFEKLKSLLSGEII
                     EKDDERFYFNWARKNQLFKLIQAPAYSTLKPVKEKSVDFDKTKNIVIIDENLENPYLV
                     NI"
     gene            complement(6898..7722)
                     /locus_tag="PLF90_06640"
     CDS             complement(6898..7722)
                     /locus_tag="PLF90_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOK56989.1"
                     /translation="MNYVEKIFSLKEKVAIITGGGGVLGSEIAKGLGLAGATVIIGDI
                     KNYKEVADNLKNSGIESEGEYLDVFDKGKIEEVAKKIVDKFGKIDILVNAAGGNIKEA
                     TTSPELSFFDLPLSALEKVIALNLFGGAILPCQVFGKYMVKNENGASIINISSMNAFR
                     PLTRIPGYSAAKAAVSNFTQWLAVHFALEYNKKIRVNAIAPGFFLTQQNRYLLLDEKG
                     NLTERGKTIITHTPMGRFGNSDELIGICIFLSSDASSFVTGTVIPIDGGFNAFSGV"
     gene            complement(7709..8734)
                     /locus_tag="PLF90_06645"
     CDS             complement(7709..8734)
                     /locus_tag="PLF90_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK56990.1"
                     /translation="MDNTFHIQWHITDNCNLRCRHCYQENFTPEKDLPFEKLTFIFSN
                     ICEFLKKRKKKLVIDITGGEPFLHNDWWELVEKIVNSEMVKDTGIITNGFFIDERIIY
                     KIEKNNIRLKLSCEGVDREVYEYFRGKNYERFINICEMIKEIKTEKFLMFTLLEKNWE
                     QIFKIFDFAREYNFDGIIIERFIPYGFGKRLKKEAISFEKWILITKFLHKICDIDFDI
                     KDIIEYRGYMVKIEKKGYELYGSECIVGKDGIAVMPDGTVFPCRRFPLPIGNLLNEKL
                     LDIWENSNVLNLIRNKAFLKGKCKNCIIDNCYGCRALVYSFYGDFLEEDPLCYLNLKT
                     GGKNELC"
     gene            8802..>10211
                     /locus_tag="PLF90_06650"
     CDS             8802..>10211
                     /locus_tag="PLF90_06650"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOK56991.1"
                     /translation="MEKFLNEFKNPGSEYRGAPFWAWNGKLKPEELRRQIRIMKEMGL
                     GGFFMHSRVGLDTPYLSKEWFECINACADEGKKQSMLAFLYDEDRWPSGAAGGLVTKD
                     KKYRMRHLVMRIFNEPKKMKDIANVVGIFLAEFEGNIIKKYRRLKKDEKIKKLSENEK
                     ILVFTKEFAKESDWYNGYTYLDTMDPEAVKQFIKITHENYKKYCGKYFEKVIPGIFTD
                     EPNYGNVYFHYEGASSDEGNIAIPWTDKLAKTFKERYGYDILDHLPEIYFEIEGQKIS
                     KVRHNYFDCITYLFVNSFAKQIGNWCEKNKLLFTGHILEEDTLLRQAHVVGNCLRFYE
                     YMQAPGMDLLTEHWRIFNTAKQVSSVARQFGRKWRLTETYGCTGWDFSFEGHKALGDW
                     QVALGINLRCQHLSWYTMLGEAKRDYPASIFYQSPWWNVYKYVEDYFARIHLVMTQGE
                     EIRDLLVIHPIESMWTVYKM"
BASE COUNT         3476 a         1809 c         1183 g         3743 t
ORIGIN      
        1 aactttatcc atttctacag gtaatccgtg aagatgaacg actataattc cttttgtttt
       61 ttctgtaatt ttttcttcaa ttttattaac atctatattc atagtcataa aatcaatatc
      121 cacgaataca ggaatacaat tgtgatgtaa aacacaggtc gcagaagaac tccatgaata
      181 tgctggaact attacttcat ctccacaacc aagttcgcat ccagcaattc ccatatgtaa
      241 agcagcagtt ccactattgg tagttattgc aaacttattt ccattccatt ccgcaaattc
      301 cttttcaaat tctttgcaat taggtccaaa actgtgaatt tctcctttta atgaagataa
      361 aacataatta atatcagatt cgtctatagg aggccacttc tttatcatcc cttcaggtat
      421 aagtttttct cctccaaaaa ttgcaagttt ttcactcatt ttcttcctcc ataattgatt
      481 ttaaaaaatt aagtcctttt tccattgcca catcaggttc atagtcattc tcttcatatt
      541 caagatttat atatccatcg taattaaaac ttttcatgac ttttataagt tttctgtagt
      601 caattattcc ctctcctaca agtgctggct tatatttttt tccatcaagt cctgacaatt
      661 ttccatcttc agaaattatc cagtccttaa aatgagtgtg tattatgtac ttttgattat
      721 ttataaatgc ttcttcaggt ttttcccctc caattattac atttccagaa tcaaaagtta
      781 ctctcaaatc aggtatttcc tttattgcaa aatttatatc atcagaaact aaaaaaggtc
      841 catttatatc agggaaatgc tcaaccgtaa tttttatctt aaatttttca ctttccttaa
      901 taacttcttt taatccttca attacatttc tccttgattc ttctcttgaa aactcctttt
      961 tccctctgag aggcaacatt attttatctg ttccaagtat tgctgcaatt tcaaatcctt
     1021 tctttatctc ttctatacct ttatctctta attttttttc tttaaagtta atatctacaa
     1081 aaaaagtata acaaacagtt ttaagtccaa aatcatccat tatttttctt acctcttcag
     1141 gtttattctc gtaagttgtt acccaatcaa ttccatcaag tccaagattt tttgtaaatt
     1201 cacacaattt ttttatactg aaatcatgtt tccagtcccc tctatacatt gtataactca
     1261 tcatactgat tttcatttac cctcccttta taatcttaca atgtacaata attatattac
     1321 actttacaaa aacaaaaaaa tgttttaaac ttaacgaggg gaaaaatggc aacaatatat
     1381 aaagatagaa ttgaaggact ttattgtaaa tgtacaaagg gggatatagc aatttttaaa
     1441 attcttgaag ataaaatcaa atgtatttac tgtggaaatg aaataaaatg caatcctcca
     1501 gttgatttta tctggaaaga aaaatgtcat cactgtgatg aaaaaaattc tacttttatt
     1561 attgactcca caggtataaa atattgctca agatgtggca actctaaata agttttctta
     1621 taattaaaaa tcccccaaaa agaataagaa agacactgaa aaaagaggaa atatatttta
     1681 aatatttttc actgattaat tttttacttt taaatataaa atacgaaaca aatgaatacc
     1741 ataaaaagtc agagaaaata tgtccggtaa aaaataaaaa aattccttta aaaccatagg
     1801 gaaatgcaaa agagagataa acaagaccta ttgtaatcca ccagattgtc cagtatggat
     1861 tgcttaaact tacaaaaata ccttcaaaaa taagagaata attactttta taagaaatat
     1921 ttatattttg ttcaattttt attttttgag aagaaaaaaa tgataaaaat cctgtataga
     1981 ttaaaaaaga gccaccaaaa atacttaaat atagttgaat catctccttt ttcaaaaaat
     2041 taccaaatcc aaaaatcaat aataatacca ttaaaagttc aagtaaagaa tgaccgataa
     2101 taattaacgg acccgcttta aaatctcttt ttattgtttc ttcaatagta acagcaagta
     2161 atggacctgg acttatagcc cctgaaaatc caattaaaaa ggatgtaaag aaaattaaat
     2221 ataatggcat ttttataagt tttcaagttc atatattaaa atagcaatac ttacaacact
     2281 tgcagtttca acttttaaaa ttctccttcc aagagaaaca gtaaaatatc catatcctct
     2341 tgcaatttca atttcttctt catcaaaacc accttcaggt ccaatgaaaa cattaatttt
     2401 cttatttttt atatctatac ctttaatttt ctcaataata ctttttattg tcacttcttt
     2461 ttctccttcc cagaaaataa tacctaaatc tttttcctct ttaatacatt cttcaaactt
     2521 attaggtttt tcaatttcag gaataaaagt cctacctgaa acttttgatg actcttctgc
     2581 aatttttaac caccttttaa ttttattctc tattttttct ttgggaattt ttacaactgt
     2641 ccttttattt attacaggga ctattctttt tactccaagt tgagttactt ctcttacaat
     2701 aaattcaaac ttatctcctt ttggtaaaca ttggaaaagt tgtaaattaa agagaagttc
     2761 cggattatag atttcttttt cttcaataat tttaccgatt atttcgtctc ttttcatttc
     2821 tttaatttca atttcatatt ctctcccgct tccatcaaat ccaataaacc tatctcctgt
     2881 ctcaaatctt aaaacatttt ttaaataatg atatgtacca ccttttatca gaaaattaca
     2941 tttaattttt acacaatctt ctccaatata tattctcctc atttcagaaa tctctctgaa
     3001 acatatttaa aaatttctcc tgacggttga taaaacttta aatccgctat attttcaaaa
     3061 taactttttt ctgtatttat ttcaactatt tttgctccat ttttttttgc tatatagggt
     3121 aagtcagaag caggctttac aaatcctgaa gtacctatga ttataaataa atctgctttt
     3181 ttactctctt cctctgcttt tttaaattca tcttctggta ataattctcc aaaaaataca
     3241 atatcgggtc tttttctttc accacaatta catcttgtaa aatcagaaat atactttatc
     3301 aaatttctcc ttccctcaat aatttttatt ttttcaacaa attcttttaa attttctttt
     3361 ccaatgtctt ccttctttcc acatttttta catttccatt tatatatatt tccatgtaat
     3421 tcaataacgt ttctactacc tgcttcctga tgtaaattat ctatattttg agttataact
     3481 gcctttaaat atcctttctt ttcaagttca gataatgtta tatgtgaaat atttggtttt
     3541 gcatttaaaa ttggataggt gaaatcataa ataaaacttg caacttttga aggagaaata
     3601 agaaattgag ttaccagacc aggtaaagtt ccataaattt ccggtttata tttttcccat
     3661 aatccagttt gtcctctgaa ttgaggtatt ccactttctg ttgatattcc tgctcctgta
     3721 aaggcaacta tatattttga attttttata taacctaaaa attcttcaat ttcattcata
     3781 aagttcttta aaaaccttaa aaccttctgt aactaaatcc tttatcatat cttcaacttt
     3841 atccgtttct ctatttgata gatattttaa aatttcatct gtattcatct tttcagtaac
     3901 tttttcacat gcttcccatg ataaaatact tcttataacc ctttcaattg ccttttcttc
     3961 attatcatag ggtcttgcct gcatatcatg tcctttccat ctgtcaaatc ctgcatcctc
     4021 aattaacttg gctataacta tattcatacc attaattctt gctccatggt caatatcaaa
     4081 ctttccaggt ccaccaagta ttattccatc attatatccc tgactgttta tatgcatgtg
     4141 aataagcatc ttattatcaa tttcttcaac cgtatcataa atatggtcaa gtcctatcat
     4201 ttctgaatgc ccgaattctt tattaactcc ctttttttca actgtaattc catattcttc
     4261 tttcaacttt ttccagaata aaatagcact tgcaactgtt ggaacaagca ttgctggatg
     4321 accttcattt ggttttggtt caatggcaaa ataaagtttc cctccttttt tctcctcgta
     4381 tttacacagt tttgcaatac tttcttttaa attatcatac atctttttaa taaatggagt
     4441 agcaatatca taaccccatg aaccattcca taaaacaaaa gttggttctt tccctttcaa
     4501 aaatacatct ttcatttttt cataagcaat atcaactgtt ttaatcccga attcttctgc
     4561 ttttttcttt tcatccgtgt ctaaagaggc aataccacca taggcaaaat gagaatgagc
     4621 accgggagta atcaaggcaa gatagatatt tttttccttc attattccaa caacttcatc
     4681 aacatttttt tcatttactt ctgaatcata atgaaactca actccaagaa taacattttc
     4741 tggcattctt ggttttattt tttctgcaac aatttttata aaatcaactg aattaaattt
     4801 atctcctgac catgaaggtc ttatatttga aggaacaaaa ccacctttcc ctggattaaa
     4861 agtccacctg caaatagaat gatatgattt tttcatattt tttcctcctt tttcacttat
     4921 tttgttactt ctttttcagg tcttccttca acaagccatt cagtatgaaa ttctccctct
     4981 tttccgtctt ctgtttttaa aatttcagga ttatcaattc ctttcaattt aaaatatcca
     5041 tgagcaccaa aataatctct ctgtaaagca cttacctgag cagaaaacat aaccggactt
     5101 gttatctgta aaatataatc ataagaattt gaaaaagcag gaactggaac tcctgcttta
     5161 tgggaaattt caataaattt tgcaagtttt ttaatatttt ttgtaataaa ttcagagaag
     5221 tttttatcaa caagaagatt tggaaggtct ggattttcct tatatgcctt tgttatttca
     5281 tcaagaaatt gtgctcttat tatacaacct gctctccata tttttgcaat ctctccaagt
     5341 tttaaatccc atttataaaa ttcacttgct gcctttaaaa gagcaagtcc ctgtgcatag
     5401 gatgaaattt tagcaatata taaagcatca tgagcaattt ttataatatt tttctttgaa
     5461 cccttaaatt tttctttaat atcaagtttt aaaagagaag aaacattcaa tcttatatcc
     5521 ttatcctgtg acatttctct tgaaaaaaca gcagaagcaa ttgttggaac aggaacaagt
     5581 aattcaagtg cactctgaac tgtccatgta cctgttcctt tcattcttgt aatatctgca
     5641 actatatcaa caagatattc atctggcttt actttatgtt tttctttcat caaatcacct
     5701 gttatctgaa ttaaataact tctcaaaata tcattttctc cattccattc aatcattata
     5761 tctgcaattt ctccagaagt cattccaaaa ccatttttga aaaaccagca acattcacca
     5821 atcaactgca tatctccata ctcaatacca ttatgtacca tctttacaaa atgacctgct
     5881 ccatcaggac caatataagt tacacacggt tctccatcat atgcttttgc agatattttc
     5941 tttaacattt cttcaacaag tttataagcc tcaaactgac ctcccggcat aatagcaggg
     6001 ccctttaacg caccctcctc ccctccagaa acaccggtac caataaatag aattcctttt
     6061 tcagaaagtt ctttatttct cctgatagta tccttgaaaa atgaatttcc accatcaatt
     6121 attaaatcac ctttttcaag taaaggaaga agttttgaaa tgaaatcatc cactggttgt
     6181 ccttctttaa ccataagcat aatttttctt ggtctttcaa gatttgtaac aaaatcagaa
     6241 atatcataag ttggtattat attttttccc tttccctgcg tttctacaaa tgcctttgtc
     6301 ttttcccctg tcctattaaa aacacaaaca gtataaccat tcctttcaaa attaagtgct
     6361 aaattccttc ccataacttc cataccaata attccaatct ctgctttttt catagttcct
     6421 ccttttttaa ctcttaattc gtttttactt gtttatatta taacccaatc tcgaatattt
     6481 tgaaagaaaa caactacaac attcataaat ttaaaaaaat gccttatttt tttaaaaact
     6541 tatttttttt gtgaataata ttaggtaaaa gatgaataaa aagataaaca aaacagtaaa
     6601 aattgatatt aaagaaggat taaagaaatt ttatccggag atttttgagg atggcaaagt
     6661 taattttgaa aaattaaaat cattacttag tggtgaaatt attgaaaaag atgacgagag
     6721 attttatttt aactgggcta gaaaaaacca attatttaaa cttatccaag cacctgccta
     6781 cagcacacta aaaccggtta aagaaaagtc agttgatttt gataaaacta aaaacattgt
     6841 tattattgat gagaatttag aaaatcctta cttggttaat atttaatcaa acttatttta
     6901 aactccagag aaagcattaa aaccaccatc tattggaata actgtgcctg taacaaaaga
     6961 agaagcatca gaagaaagga atatacaaat acctataagt tcatctgaat ttccaaatct
     7021 tcccattgga gtatgtgtaa ttattgtctt tcctctttct gttaaattcc ccttttcatc
     7081 cagtaataaa tatctatttt gctgtgttaa aaaaaatcca ggagcaattg cattaactct
     7141 tattttttta ttgtattcca aagcaaaatg aacagcaagc cactgtgtaa aattactcac
     7201 cgctgctttt gctgctgaat atcctggaat tctcgttaaa ggtctaaaag cattcattga
     7261 agagatatta attatagaag caccattttc atttttaacc atatattttc caaatacctg
     7321 acatggtaaa atagcacctc caaaaagatt aagagcaatt actttttcaa gagcagataa
     7381 aggtaaatca aaaaaagata gttcaggaga agttgttgcc tctttaatat tcccaccggc
     7441 agcattcaca agtatatcaa tctttccgaa tttatcaaca attttttttg caacttcttc
     7501 aatctttcct ttatcaaaaa catcaagata ttctccttca ctttcaattc ctgaattctt
     7561 taaattatct gcaacttctt tgtaattctt aatatctcca ataattacag ttgcaccggc
     7621 aagtcccaag ccttttgcta tttcagaacc taaaactcct cctccaccag ttataattgc
     7681 aactttttct ttcaaagaaa aaattttttc aacataattc atttttccct cctgttttta
     7741 agtttaaata acataatggg tcttcttcaa gaaaatctcc ataaaaactg taaacaagag
     7801 ccctgcaacc ataacaatta tcaattatac aatttttaca tttacctttc aaaaatgcct
     7861 tatttcttat aagatttaaa acatttgagt tttcccatat atctaaaagt ttctcattca
     7921 ataaattccc tataggtaat ggaaatctcc tgcaaggaaa aacagttcca tcaggcataa
     7981 cagcaatacc atcttttccc acaatacact cacttccata aagttcatac cccttttttt
     8041 caattttaac catatatccc ctgtattcaa taatgtcctt tatgtcaaaa tcaatgtcac
     8101 atattttatg taaaaatttt gtaattaaaa tccatttttc aaaagagatt gcttcttttt
     8161 ttaatctttt accaaaacca tagggaataa atctttcaat aatgatacca tcaaaattat
     8221 attctcttgc aaaatcaaaa attttaaaaa tctgttccca gtttttttca agcaaagtaa
     8281 acatcaaaaa tttttctgtc tttatttctt ttatcatttc acatatattt ataaaccttt
     8341 cataattttt acctctgaaa tattcataaa cttctctatc cactccttca caggacaatt
     8401 ttaatcttat attatttttt tcaattttgt aaataattct ctcatcaata aaaaaaccat
     8461 ttgtaattat tcctgtatct ttaaccattt ctgaattcac aatcttttct accaattccc
     8521 accagtcgtt atgtaaaaaa ggttctccac ctgttatatc tattacaagt tttttttttc
     8581 ttttttttaa aaattcacag atatttgaaa aaataaaagt cagtttttca aaaggaaggt
     8641 ctttttctgg tgtaaaattt tcctgataac aatgtctaca ccttaaatta caattatccg
     8701 tgatatgcca ctgaatatga aatgtattat ccataaaaat attttacttt tttttaaata
     8761 agatataaaa tttcaaaact aaataaaaac aggaggaaaa aatggaaaaa tttttgaatg
     8821 aatttaaaaa tcctggtagt gagtatagag gagctccatt ctgggcatgg aatggaaaac
     8881 tcaaaccaga agaattaaga aggcagataa ggataatgaa agagatggga cttggtggtt
     8941 tctttatgca ttcaagagtt ggacttgata ctccatattt gtcaaaggaa tggtttgaat
     9001 gtataaatgc ctgtgccgat gaagggaaaa aacagagtat gctcgccttt ctttacgatg
     9061 aggatagatg gccatccggt gctgcaggag gacttgttac aaaagataaa aaatatagaa
     9121 tgaggcatct tgttatgaga atttttaatg aacccaaaaa aatgaaagac attgcaaatg
     9181 ttgttggaat ttttcttgca gagtttgaag gaaatattat aaaaaaatac agaagattaa
     9241 aaaaagatga aaaaataaag aaattatctg aaaatgaaaa aatacttgtt tttacgaagg
     9301 aatttgcaaa agaaagtgac tggtataatg gttatacata tcttgataca atggatccgg
     9361 aggcagttaa acaatttatc aaaattacac atgaaaatta taaaaaatat tgtggaaaat
     9421 attttgaaaa agttatacca ggtattttta cagatgagcc aaattatgga aatgtttatt
     9481 ttcattatga aggggcatct tctgatgaag gaaatattgc tattccatgg actgataaat
     9541 tagcaaaaac atttaaagaa aggtatggat atgatatttt agaccatctt cctgaaattt
     9601 attttgaaat agaaggacaa aaaatatcaa aggtaaggca taattatttt gactgtatta
     9661 catatctttt tgtgaattca tttgcaaaac agataggaaa ttggtgtgag aaaaataaac
     9721 ttctttttac aggacatatt cttgaagaag atacactttt aaggcaagca catgttgttg
     9781 gaaattgttt aaggttttat gaatatatgc aggcacctgg aatggattta ttaacagaac
     9841 actggagaat ttttaataca gcaaaacagg taagttcagt tgcaagacag tttggaagaa
     9901 aatggagatt aacagaaaca tatggatgta ctggctggga cttttcattt gaaggacata
     9961 aagcactggg tgattggcag gtagcactcg gtataaattt aagatgtcaa cacctttcat
    10021 ggtatacaat gcttggagaa gcaaaaagag attatcctgc aagtattttt tatcaatctc
    10081 cctggtggaa tgtttataaa tacgttgaag attattttgc aagaatacat cttgttatga
    10141 cacagggaga agaaataagg gacttacttg taattcatcc aattgaaagt atgtggactg
    10201 tttataaaat g
//
LOCUS       DAOSAT010000067         9758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_4565_length_9758_cov_4.162115, whole genome shotgun sequence.
ACCESSION   DAOSAT010000067 DAOSAT010000000
VERSION     DAOSAT010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4565_length_9758_cov_4.162115"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4069)
                     /locus_tag="PLF90_06655"
     CDS             complement(<1..4069)
                     /locus_tag="PLF90_06655"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOK56992.1"
                     /translation="MKKVLMFLVFLSFTIFAGTDAYFKDTDGDGKDDVLIMENEYYRI
                     TFTPQEGAKAKEVLYKPANKIISTTSAWFQDHQIELGDMVGSKLYYCNHSYNAEIIKK
                     GPDICEIKFYANLPGVGKYASYKDVLIERTYKLNQNSPIINVSINVKNNSKEALPFTL
                     MVGHWAWIENEDSWYFVPDDLGVINDFDSQVRTFSAPVGSQEPTSNFTGFISVQSKLG
                     LIFVMDWKYLDAIECWLSKGKGACIQWPYRKQNLRPGEIWSTRYIIYPVENIESIEAA
                     DENYTLGITVGSESGIGNFISKKEIQAGKEVPVKVYISGPYGGKIKVEYGYRILPEEK
                     ETIIGSKELQLDKIKGTSFQFPLLFEKKDCTYVVKVRIKDEKNNILEGEKPVKVGDSS
                     YTYFMKRKPEPQIGEKYYGYQIINPPLPSWYEEVDLKVETPHTKWAKPWCNGKMNVLF
                     VNRSDNSVGYWREIWQRCDIDFDCCSFAFDEAKKYPYTQNTLKKLLKLLNEKNYDVIF
                     FAGLHWDEGIPQYIQDSIFSFIKKGKGAVILGDLTNKQIYGTLDKYLRENGKEIDSKW
                     ITVGIPYRMPKVWLFELGNGRVVVIQGNPGRGYETVSPSLGDWQAEGRWMWIPGWEYG
                     FGLFSRSIIWASKRESGITAKEIKGSPEKIEFVINNQTGRDIETTYNLKVYNGFYEVE
                     EEKNGKIKLASGENKIELKIEKSLTDKVHIADLILKDTKGRILEWASCKFDVKKDITL
                     TVKMDRDTSAYRSNEEIKGIITIENKGEKKNLKLDIEVVDAYNRVVYKEQKNIEVNPG
                     KNEVSLSIDKGKILDIWHELRVSLLDKDLSITKDRHIFFIYPDRMPLYDDFYLACWGN
                     LEPDPLKIIIAGDKLKEAGIDYVYSYGEGKGERYVAYKTHHLLMGPPFSCSLKGGYFG
                     NRKADTKNLTYDPPLVPSDEEITKFKERMRNLAKGYSEWGGADYIHLDDERDMQGDYD
                     WSERTINKFREWLKENYKSIDALNKQWETNYKDWNEVMPIRISEIKDMNNISQYLDWR
                     IFIGWAIIEYYYKVPAEAVKEGNPRGVVGQHGIYQPSLTIPQDFWLISKYTPVTGRYN
                     GMLEEWFSSFGVISGQYGGYGVEKATPGHRFHSWRSLLHGGHWAFYYILWNAGTHHQG
                     ILSPDQTVHGGYNDLSKDEFSDIKKGIGKLFIETEFSDDGICFPYSHSSILASQVLGL
                     PRTGNLYSQKTLIENLGYQHRTLSYEQIKNGELKNFKLLILPNTICLSKGEIDAIKDF
                     VKSGGIVIADYQPGVRDEHGKIYGKKGPLDEVFGIDRSSAEFIKRKMKIKFLKESIFG
                     EKELEMNIAETGVKTTTGKNMAVFEDGTPALIFNNY"
     gene            complement(4084..5712)
                     /locus_tag="PLF90_06660"
     CDS             complement(4084..5712)
                     /locus_tag="PLF90_06660"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOK56993.1"
                     /translation="MKRINTLELIKKILILSFFLNISYLFSSDIDAKKYINYDLPDCG
                     LQKAIDDCFLSGGGTVKIPEGVFKLRRGLLLKNKVNIEGSGIDKTVLIYGKEILKINV
                     TKENEGNKIFLEYIPEKMEIGSAVVLCRIFPPSWYGEPKPGYVKEINKEEKYIIVEAP
                     YGMFQLKPPNGCIIFGDSAYPDKDVKKGDIEIYLKSTSIFKEGEEIAIGEPPNESLLA
                     HAFIKEIKENKIILESPVSIDFKANPSSEELGNKKINVLIWAVFPLIHGANVEDIKIS
                     NLTIRGNAKGNAYTTQGRYTVAGIHIYNGKNIVIEKIKVTEWPTDGISLQTGENCIVR
                     DCEVIGCLGNGIHPGTGLKNTLIENNKIFENGQGIYFCWHNFGHKIKNNIIGRNKSHG
                     IGGLGNPGDTNNLIENNHIFENGGAGIEINGGKKSNNVIVNNIIENNSKSQPGKFPGI
                     MLYASSEDAMNYTIKGNKIRDTQNEKTQHIGIEEKNGMYRDKPTSADFNIIEYNELSG
                     HLKADIIIVGKNTVCKNNKADKIVLPEEEKKDEK"
     gene            complement(5826..6461)
                     /gene="rnc"
                     /locus_tag="PLF90_06665"
     CDS             complement(5826..6461)
                     /gene="rnc"
                     /locus_tag="PLF90_06665"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOK56994.1"
                     /translation="MQELEKIIGYRFRNKKYLEEALTHPSYSPDSSYERLEFLGDLIL
                     DTVVGIYLYKKYKDKNEEFLTNLKSSYVNNNYLKKICDEIQIMKFARHKNCNIKRTDR
                     FLEALIGALYLDGGWIAVKKFIKNFILNRELEPLKDYKNLLLNYARKTKQVSPEYKII
                     SEKGFPHKKEFVVKVKIKGIRKIGKGKGESIKEAELLAAKELYEKLMNLKK"
     gene            complement(6449..7900)
                     /gene="lnt"
                     /locus_tag="PLF90_06670"
     CDS             complement(6449..7900)
                     /gene="lnt"
                     /locus_tag="PLF90_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOK56995.1"
                     /translation="MRKIRLKNNFFLNSGICILSSILMFFSFPPFKFWYFSFFCLIPL
                     FLICGKNNFLNFIYGLISGFIFYSLSLYWLKNVSGFIYLLLALYLSIYWAIFTFLIFL
                     FKKEKIIFLGASIWFFLELIMANLLTGFPWLVFGLTQYKNLYISQVAKFTGIFGISFI
                     LVASNLFIYSIIKREFLSQQLFFIFFIIFCFLITKLPQKKEIKGRLKISLVQPNFAIY
                     ENTLEECKEKIIFYIEKKVRHDKPNIIIFPEGSFQGDIFEEKGFINKLKDLSRKNNFG
                     ILIGTFTKKDDYFYNSSVFINSERIEVYNKIHLVPYGEFILGEKFNFVKNIFLKIAGY
                     YPKLKRGNEFKVFTFREIKFSTPICYENIFPEISENFLKNGSDFFIVITNDSWFGNSS
                     GPYQHFYHNVFRAIETGRYFLQAGLTGMTGIIGPEGKIEKILEKNNNYLFFEDVLTWS
                     LPVVIYETFYSKYGIYPFLLFCLILTGLLICRN"
     gene            complement(7875..8222)
                     /locus_tag="PLF90_06675"
     CDS             complement(7875..8222)
                     /locus_tag="PLF90_06675"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOK56996.1"
                     /translation="MRIMTRCKIKGGKVTDKNLYYEGSITIDRKIIQEADIVPGEMVY
                     VLNLNNGARIFTYVIEGEENSGTICLNGPSARFFEVGDEVIILGISLVEEEKIKDFKM
                     RIVKLGEKNKIEK"
     gene            8337..9266
                     /locus_tag="PLF90_06680"
     CDS             8337..9266
                     /locus_tag="PLF90_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK56997.1"
                     /translation="MIKYFQKVNSETYQLLGYLGFTKEESFKTNSGEWEIYTKTNFIF
                     PHFISEFNPPLNKFCAILKNTKEFIVYGTFETLEEVKKKLIQLSDIFFKKEKKFFGIP
                     LTLTEENAQDYGYIRGLILGFLLLIFDFSYPFIFKLKSGIINSFIDYVKIIYFGTPGF
                     GIFVGITGTGLYFIFLLIIIPIFFGWYYKKLAIKKNIRKIDRFIKDISGYDYDFGIDA
                     EKTLKDEFTLIIEEKKKEQIYNELKKSVDVSKQDFENVYEKLKTGFFNISDMLEFIDL
                     YEKTFKNFSLKKLLNIIAKYDTARYLTEIKFQI"
     gene            9332..9547
                     /locus_tag="PLF90_06685"
     CDS             9332..9547
                     /locus_tag="PLF90_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOK56998.1"
                     /translation="MEKIFGKILIVIGLLIFLVGYVIYKGIKIPIGRLPGDIFIKREN
                     FVFYFPIVSSILISIILTIIFSLLWRR"
     gene            9549..>9758
                     /locus_tag="PLF90_06690"
     CDS             9549..>9758
                     /locus_tag="PLF90_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOK56999.1"
                     /translation="MERKNGWEIWDESKIKEVFAYCEDYKEFLSINKTVRKFVKSCIE
                     KSKEKGFKNIDELNVIKKGDKFYKIN"
BASE COUNT         3183 a         1687 c         1075 g         3813 t
ORIGIN      
        1 cataattgtt aaatattaaa gcaggtgtcc catcttcaaa aacagccata ttttttccag
       61 ttgttgtttt tacacctgtt tctgcaatat tcatctcaag ttccttttct ccaaaaatgc
      121 tttcttttaa aaatttaatc ttcatttttc tttttataaa ttctgcactg ctcctatcta
      181 ttccaaaaac ttcatctaaa ggcccttttt tcccataaat ctttccatgt tcatctctta
      241 ctccaggttg ataatcagca ataacaattc ctccactttt tacaaaatct tttattgcat
      301 caatttctcc ttttgaaaga catatcgtat ttggtagaat tagaagttta aagtttttaa
      361 gttctccatt tttaatctgc tcataagaaa gtgttctgtg ctgatatcca aggttctcaa
      421 ttaatgtttt ctgactgtat aagtttcctg ttcttggtaa tccaaggacc tgtgaagcaa
      481 gaattgaaga gtgagaataa ggaaagcata taccatcatc actgaattct gtctctataa
      541 aaagtttacc aatacccttc tttatatctg aaaattcatc ttttgaaagg tcattatagc
      601 caccatgaac tgtctggtca ggtgaaagaa ttccttgatg atgggttcct gcattccata
      661 aaatataata aaaagcccag tgtcctccat gcagtaaact cctccatgaa tgaaatctat
      721 gtcctggtgt tgctttttcc actccatacc caccatattg acctgaaata actccaaatg
      781 atgaaaacca ttcttcaagc atgccattat atcttcctgt aacaggtgta tattttgata
      841 ttaaccagaa atcttgaggt attgtcaaac ttggctgata tattccatgc tgaccaacta
      901 ctcctcttgg atttccttct tttactgcct ctgctggaac tttataataa tattcaataa
      961 tcgcccatcc aataaatatc ctccagtcaa gatactgtga aatattattc atatccttta
     1021 tctccgaaat tcttataggc ataacctcat tccagtcctt gtaatttgtt tcccattgtt
     1081 tattaagtgc atcaattgat ttataatttt cttttaacca ttctctaaac ttatttattg
     1141 tcctctctga ccagtcatag tcaccttgca tatccctttc gtcatcaaga tgtatataat
     1201 ctgctcctcc ccattcagaa tatccttttg ctaaatttct cattctctct ttaaatttag
     1261 taatttcttc atctgatgga acaagtggtg ggtcataggt taaatttttt gtatctgctt
     1321 ttctatttcc aaagtatccg ccttttaaac tgcaggaaaa aggtggtccc attaataaat
     1381 ggtgtgtttt ataggcaaca tacctttcac ctttaccttc tccatatgaa taaacataat
     1441 caatacctgc ttctttaagt ttatcacccg ctattattat ttttaatggg tctggttcaa
     1501 gatttcccca gcaggcaaga taaaaatcat catataaagg cattctatca ggatatataa
     1561 agaaaatgtg tctatccttt gtaatactta aatctttatc taaaagagaa actcttaatt
     1621 catgccagat atcaagaatt ttacccttat ctatacttaa agatacttca tttttccctg
     1681 gatttacttc tatattcttc tgttctttat aaacaactct gttatatgca tccacaactt
     1741 caatatcaag ttttaaattt ttcttttctc ctttgttttc aatagtaata atccctttta
     1801 tttcttcatt acttctgtat gctgatgtat ctctgtccat ttttactgtt aatgttatat
     1861 ctttttttac atcaaactta caacttgccc attccaaaat ccttcctttt gtatctttca
     1921 aaatcaaatc agcaatatga actttatctg taagtgattt ttctatcttc aattcaatct
     1981 tgttttctcc tgatgctaac tttatttttc catttttttc ttcctctacc tcataaaatc
     2041 cattataaac tttcaaatta taagttgtct ctatatctct tcctgtttga ttatttatca
     2101 caaattctat tttttctgga cttcctttta tctcttttgc agttattcct gattctcttt
     2161 tacttgccca tattatactt cttgaaaata acccaaatcc atattcccat ccaggtatcc
     2221 acatccatct cccttctgcc tgccagtcgc ctaaacttgg actgactgtt tcatatcccc
     2281 ttcctggatt tccctgaata actacaaccc tgccatttcc aagttcaaat aaccatactt
     2341 ttggcatcct gtatggaata ccaactgtta tccactttga atctatttct ttgccattct
     2401 ctcttaagta tttatctaat gttccatata tctgcttgtt ggttaaatcg ccaagtataa
     2461 ctgctccttt accctttttt ataaatgaaa atatgctatc ctgaatatat tgtggaatac
     2521 cttcgtccca gtgtagccca gcaaaaaata taacatcata atttttttca tttaatagtt
     2581 taagaagttt ctttaatgtg ttttgtgtat atggatattt ttttgcttca tcaaaagcga
     2641 atgaacagca atcaaaatct atatcacatc tctgccatat ttctctccag tatccaactg
     2701 aattatcact cctgtttaca aataaaacat tcatctttcc attacaccag ggctttgccc
     2761 attttgtatg aggtgtttca acttttaaat caacttcttc ataccaggaa ggaagtggtg
     2821 gatttatgat ttgataacca taatatttct ctcctatttg tggttctggt ttcctcttca
     2881 taaaatatgt atatgaagaa tctcctactt ttaccggttt ttctccttca agtatattat
     2941 ttttctcatc ttttattctc acttttacaa catatgtaca atccttcttt tcaaatagaa
     3001 gtggaaattg aaaactcgta ccttttattt tatccagttg taattcttta cttcctataa
     3061 tcgtttcttt ttcttcaggc aatatcctgt atccatattc aacttttatt tttccaccat
     3121 aaggacctga aatataaact ttaacaggaa cttctttacc tgcctgtatc tctttctttg
     3181 aaataaaatt acctatacct gattcactac caactgttat tcctaatgta taattctcat
     3241 ctgctgcttc aatactctct atattctcaa caggataaat tatatatctt gttgaccata
     3301 tctctccagg tcttaaattc tgttttctat aaggccattg aatacatgca ccctttcctt
     3361 tcgataacca acattcaatt gcatcaagat atttccagtc cattacaaat attagcccga
     3421 gtttgctctg aacacttata aaaccagtaa aatttgaagt cggttcctga gaaccaacag
     3481 gagcactaaa agttcttact tgactgtcaa aatcatttat tactccaagg tcatctggaa
     3541 caaaatacca actatcttca ttctctatcc atgcccaatg tcctaccata agtgtaaatg
     3601 gtaatgcttc ttttgagtta ttttttacat ttatggaaac atttataatc ggtgaattct
     3661 gatttaattt gtaggtcctt tctatcaaaa catctttata agaagcatat ttaccaaccc
     3721 ctggtaaatt agcataaaat tttatttcac atatatcagg accttttttt attatttctg
     3781 cattatagga atggttacaa taataaagtt tacttccaac catatctcca agttcaatct
     3841 gatggtcctg aaaccaagca gatgttgtac ttataatttt atttgctggt ttatataata
     3901 cttcttttgc ttttgctcct tcctgtgggg taaatgtaat cctgtaatat tcattttcca
     3961 ttatcagaac atcatctttc ccgtctccat ctgtatcttt aaaatatgca tctgtacctg
     4021 caaaaattgt aaaacttaaa aataccagaa acattaaaac cttcttcatt ctatccctcc
     4081 tttttatttt tcatcttttt tttcttcctc cggtaaaaca attttatccg ctttattatt
     4141 tttacatact gtattttttc caacgattat aatatctgct tttaaatgtc ctgataattc
     4201 attatactca attatattaa aatctgcaga agttggttta tccctgtaca ttccattctt
     4261 ttcttcaatt ccaatatgtt gtgttttttc attctgagtg tctcttattt tattcccctt
     4321 tattgtatag ttcattgcat cctctgaaga agcataaagc ataattcctg gaaattttcc
     4381 gggttgtgac tttgagttgt tttcaattat attgtttact attacattat ttgatttctt
     4441 ccctccattt atctcaattc cagcacctcc attttcaaag atgtgattat tttctattaa
     4501 attatttgta tctcccggat ttccaagtcc acctattcca tgtgatttat tccttccaat
     4561 aatattgttt ttaatcttat gcccaaaatt atgccagcag aaataaatac cctgaccgtt
     4621 ttcaaaaatt ttattatttt ctataagtgt atttttaagc cctgttccag gatggattcc
     4681 attaccaaga catcctataa cttcacaatc cctgactata caattttctc ctgtctgtaa
     4741 acttattcca tcagttggcc attcagttac ttttattttc tcaattacta tatttttccc
     4801 attgtaaata tgaattccag caactgtata tcttccttgt gttgtatagg catttccttt
     4861 tgcatttcct ctaattgtta aattacttat ttttatatct tcaacatttg ctccatggat
     4921 taatggaaaa actgcccata ttaaaacatt tatcttttta tttccgagtt cttcagagga
     4981 tggatttgct ttaaaatcaa ttgaaacagg tgattcaaga ataattttat tttcttttat
     5041 ttctttgata aatgcatggg caagaagaga ttcatttggc ggttcaccta ttgcaatctc
     5101 ttctccttcc ttaaaaattg atgtactttt aagataaatt tcaatatctc cttttttaac
     5161 atctttatca ggataagcag aatcaccaaa aattatacat ccatttggag gttttaattg
     5221 aaacatccca taaggtgctt caacaattat atatttctct tctttattta tttcttttac
     5281 ataccctggt tttggttctc cataccatga aggaggaaat attctacata aaacaactgc
     5341 acttccaatt tccatcttct caggtatata ttcaagaaat atcttgttcc cttcattttc
     5401 tttcgtaaca tttattttca aaatctcttt tccatatatt aaaactgttt tgtcaattcc
     5461 acttccctca atatttactt tgttttttaa caaaagtcct cttctcaatt tgaaaactcc
     5521 ttcaggaatt ttaacagtcc cacctcctga tagaaaacaa tcatcaatcg ctttttgaag
     5581 tccacaatca ggaaggtcat aatttatata tttctttgca tctatatcag aagaaaaaag
     5641 gtatgaaatg tttaagaaaa aagaaagaat taaaattttt tttattaact ctaatgtatt
     5701 aattcttttc atttcaaatt tattacccat catttttaat taaaacataa attttaaatt
     5761 tgtcaaattt ttttcttttt tgtaaatata ttttaaaatt aattattctc aatttttact
     5821 gctttttatt tttttaaatt cataagtttt tcataaagtt cttttgcagc aagtaattct
     5881 gcttctttta tgctttcccc tttacccttc cctatttttc taatcccttt aatttttact
     5941 tttacaacaa actccttttt gtgtggaaaa cccttttcag aaataatttt atattcagga
     6001 gatacttgtt ttgtttttct tgcataattt aaaagtaaat tcttataatc tttcaatggt
     6061 tcaagttctc tatttaaaat aaaatttttt ataaattttt taactgctat ccatccacca
     6121 tcaagatata atgcacctat taatgcttca agaaatctgt ctgttctctt aatattacag
     6181 tttttatgtc ttgcaaattt cattatctga atttcatcac atattttttt taaataattg
     6241 ttgttaacat aagatgattt taaatttgtt aaaaattctt catttttatc cttatacttt
     6301 ttgtataaat aaattcctac aactgtatcc agaatcaggt caccaagaaa ttcaagtcgt
     6361 tcatatgatg aatcaggtga gtatgaagga tgagtcagtg cttcttcaag atatttttta
     6421 tttctaaatc tatatccaat tattttttct aattcctgca tattaaaagc cctgttaaaa
     6481 ttaaacagaa taataaaaaa ggataaatgc cgtattttga atagaaagtt tcataaataa
     6541 ctaccggtaa tgaccatgtt aacacatctt caaaaaataa ataattatta tttttctcaa
     6601 gtattttttc tatttttcct tccggaccta ttatacctgt cattcctgtc aatcctgcct
     6661 gtaaaaaata tcttcccgtt tcaattgccc tgaaaacatt atgatagaag tgttgataag
     6721 gcccagaaga atttccaaac catgaatcat ttgtaattac aataaaaaaa tcacttccat
     6781 tctttaaaaa attttcactt atttcaggaa aaatattttc ataacaaatc ggtgttgaaa
     6841 attttatttc tctaaaggta aacactttaa attcattccc tctttttaat tttggataat
     6901 aaccagcaat ttttaaaaat atatttttta caaaattgaa tttttcgcca agtataaatt
     6961 caccataagg aacaagatga attttattat aaacttcaat tctttcagaa tttataaaaa
     7021 cacttgaatt gtaaaaataa tcatcttttt tcgtaaaagt acctattaaa attccaaaat
     7081 tattttttct ggataaatct ttaagtttat taataaatcc tttttcttca aaaatatctc
     7141 cctggaaaga accctcaggg aaaataataa tattcggttt atcatgcctc actttttttt
     7201 caatataaaa tataatcttt tctttacatt cttccaatgt gttttcgtaa atagcaaaat
     7261 ttggctgaac taaagaaatc tttaatcttc ctttaatttc ttttttttga ggcaattttg
     7321 taattaaaaa acagaagata ataaaaaaaa taaaaaaaag ttgttgtgat aaaaattctc
     7381 tctttattat actgtaaata aatagatttg aagcaactaa aataaaagat ataccgaaaa
     7441 tcccagtaaa ttttgcaacc tgtgaaatgt agagattttt atattgagtt aaaccgaaaa
     7501 caagccatgg gaatcctgtt aaaagatttg ccattattaa ttcaaggaaa aaccagattg
     7561 atgcacctaa aaatataatc ttttcttttt taaataaaaa aattaaaaaa gtaaaaattg
     7621 cccagtatat tgagagatat aatgctaaaa gtaaataaat aaaacctgat acattcttga
     7681 gccagtataa agacaatgaa taaaaaataa aaccagaaat taatccataa ataaagttta
     7741 aaaaattatt ttttccacat attaaaaaaa ggggaataag acagaaaaaa gaaaaatacc
     7801 agaatttaaa aggaggaaaa gaaaaaaaca tcaaaattga acttaaaata cagattcctg
     7861 aattcagaaa aaaattattt ttcaatctta tttttctcac caagtttaac tattctcatc
     7921 ttaaaatctt ttattttttc ttcttcaaca agtgaaattc ctaaaataat aacctcatct
     7981 ccaacttcaa aaaatcttgc agatggaccg tttaaacata ttgttcctga attttcctct
     8041 ccttcaataa catatgtaaa aattcttgct ccattattta aatttaaaac ataaaccatt
     8101 tcaccaggta caatatccgc ttcctgaata atttttctat caattgtaat actcccttca
     8161 taatacaagt ttttatctgt aacctttcct ccttttattt tacatcttgt cataattctc
     8221 atttttgctt cctttctgaa ttaaatattt tacaacattt tatttttttt acaatggact
     8281 taaatgtttt tttattataa aataaaatca tgtccgatgt taaattgatt tacgaaatga
     8341 taaaatattt tcaaaaagtc aattcagaaa catatcaatt actcggatac ctcggcttta
     8401 caaaagaaga gtcatttaaa accaattcag gagaatggga gatatatact aaaacaaatt
     8461 ttatattccc tcattttatc agtgaattca atccaccttt gaataaattc tgtgctattt
     8521 taaaaaatac aaaagaattt atagtgtatg gaacatttga aactcttgaa gaagtgaaaa
     8581 aaaaattaat tcaactttct gatatatttt ttaaaaaaga aaagaaattt tttgggattc
     8641 cacttacact tacagaagaa aatgctcagg attatggata tataagaggt ttaattcttg
     8701 gttttttatt actcatattt gatttttctt atccttttat ttttaaactt aaatcaggaa
     8761 ttataaacag ttttattgat tatgttaaaa tcatatattt tggaactcct ggttttggta
     8821 tttttgttgg gattacagga actggtttat acttcatctt tcttttaata attataccaa
     8881 ttttttttgg atggtattat aaaaaattgg caattaaaaa aaacataaga aagatagata
     8941 gatttattaa agatatatct ggttatgatt atgattttgg aattgatgct gaaaaaacat
     9001 taaaagatga atttactctg attatagaag aaaagaaaaa agaacagata tataatgaat
     9061 taaaaaaatc tgttgatgta agtaaacagg actttgagaa tgtatatgaa aaacttaaaa
     9121 ccggtttttt taatataagc gatatgcttg aatttattga cctttatgaa aaaacattca
     9181 aaaacttctc tttaaagaaa cttctcaata taatcgcaaa atacgatact gcaagatatc
     9241 tgacagaaat taaatttcaa atttagcaat attaaagttt gttttaattt ttttttatgg
     9301 taaaataata taaggttttt tatcaaaaaa aatggaaaaa atattcggga aaatacttat
     9361 agttattggg cttttaattt ttctggttgg atatgtaatt tataaaggta ttaaaattcc
     9421 tattggacga cttccaggag atatcttcat aaaaagagaa aattttgttt tttattttcc
     9481 aattgtttca agtattttaa tcagtataat tttaactata attttttcat tattatggag
     9541 gaggtaaaat ggaaagaaaa aatggctggg aaatatggga tgaatctaaa ataaaagaag
     9601 tttttgcata ctgcgaagat tataaagaat ttctctccat aaataaaaca gttagaaaat
     9661 ttgttaaaag ttgtattgaa aagagtaaag aaaaaggatt taaaaacata gatgaattaa
     9721 atgtaattaa aaaaggtgat aaattttaca aaataaat
//
LOCUS       DAOSAT010000068         9576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_4680_length_9576_cov_7.015019, whole genome shotgun sequence.
ACCESSION   DAOSAT010000068 DAOSAT010000000
VERSION     DAOSAT010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9576
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4680_length_9576_cov_7.015019"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PLF90_06695"
     CDS             <1..596
                     /locus_tag="PLF90_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57000.1"
                     /translation="SVARQFGRKWRLTETYGCTGWDFSFAGHKALGDWQIALGINLRC
                     QHLSWYTMLGEAKRDYPASIFYQSPWWNAYKYVEDYFARIHLVMTQGEEVRDLLVIHP
                     IESMWTVYKMPDWKNEEKKWEYSDEVKKLDEMFVKLCDTLLSSHIDFDYGDEEILSRL
                     AKIQKKGNQTILKVNKAEYKTILVPPLYTIRSSTLEI"
     gene            844..2088
                     /locus_tag="PLF90_06700"
     CDS             844..2088
                     /locus_tag="PLF90_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57001.1"
                     /translation="MEWDINIGKYCLVRDRKREFKNVIIKNFPDWKYRPIELNPQTGE
                     IYYPETIKDENGYKIKTDFYALSSHLYVIPKKKEINLDIKETKKYEIVRNLKIEKEKW
                     EYILTEKNVLVLDMPYFKIENGNWQEKKEILKVDREVRRFLGLSPRGGAMVQPWARKK
                     NKNPKSVKVSLKYEFHIEELPKGEIKIALEKPELYEIYINGYKLSSDMKSGWWVDKSL
                     ETINFAPSILKIGKNEIILETDYNENHPGFEIIYLLGDFGVKLVENDLIITGLPETLK
                     TGDWTEQGLPFYSGNVGYISVIEKIDCDEKEKIFLKIPEFRGVGIRIFIDGKEVGVVA
                     WPPCEIDITDYVKGKDRINLIIEILGHRRNSHGPLHYSEKWPVWTGPYQFVSEGNLWK
                     DKYQIVPCGLMENPEIIIKKEK"
     gene            2091..2942
                     /locus_tag="PLF90_06705"
     CDS             2091..2942
                     /locus_tag="PLF90_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOK57002.1"
                     /translation="MFRNLSPEAINIKTTILERLRLAKLGNFEGMDISILESFEFSRV
                     DKVEKIKSMFESFDIKIGGWCLPFNIGETEERFNQNIEMLNEYLKITQTLGAFRIYSW
                     ILPFSDDLPFKDNFQLHLQRAKKVCQIMEKYNCKLGLEFIGTKKVREKHKYEFIWNLE
                     QMCEFLKEVSMDNIGILLDSWHLYASDGKIEDIKKLKGKDIIYVHVNDAPNIPKENLI
                     DNERFLPGETGVIDIVEFLKALKEIEYDGPVTPEPFNKRVNELPDEIAVRLTGGYLLK
                     IWNKVFK"
     gene            2953..4575
                     /locus_tag="PLF90_06710"
     CDS             2953..4575
                     /locus_tag="PLF90_06710"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOK57003.1"
                     /translation="MGYIYFPQFKNGKNGLKIHPGLTILDFAQKLKVKINAECGGIGK
                     CGKCVVRIEKGEENLNQLTLSEKKSNLPEKHRLACQARIVRDVLDIIVYIKEFGEYEI
                     LKYGMEREVEICPVYQKKEDKVCKDNKEIDVYRNGIFGLAVDIGTTTIVFDIVNLENG
                     DIIGTIAKTNPQIIFGNDVISRIEYVMVDKKEKRFLNPDERYQKLLELQKVVVDLINE
                     SIKEFNNDIKNSIYQAVIVGNPTMRDIFFLHDISPLGKTPYEPLILQGITKKPEEIGL
                     DINKNGEIYGLPLIGGHVGADIVADILASEIYNEEKVCMIIDIGTNGEIVIGNKEKLF
                     ATSCAAGGAFEGVNISCGIGGIEGAIKEVKILNGKVYYSTIGNKYPLGICGSGLIDLL
                     SEMLKNKIIDEKARFRNEINEFYITGDIKINQQDIFQLITSKAAIRTAWQILSKIYPV
                     EIEKIEKIYLSGGFGNFINIESAINIGLIPDIEREKIIKIGNGAIEGAREVLLCSDKR
                     KLAEDIARKVKHIKTNEIEKDFDFLLASNMYF"
     gene            complement(4580..5653)
                     /locus_tag="PLF90_06715"
     CDS             complement(4580..5653)
                     /locus_tag="PLF90_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOK57004.1"
                     /translation="MELKKDCKYALLVPTSMGVRITPFNMQPVHTSNLYIMQATSAET
                     NVASVSSYLGMPVKVLTAFVKGSPIARFIKENLKSRGMDVEYKEVLQGGPWGFRHQFN
                     IADTGYGSRAPRVWNDRTGEVGRTLKPEDFDLERIFGKEGVQIVHMSGLFAALSPETG
                     NFCLEISKIAKKYGTRISFDLNYRASFWKNREKELREIFTEIARVTDILVGNEEDFQL
                     CLGIEGPEPGGKNIKAQIENFKGMIERTKKQFPNVEVFATTLREVVSAQQHLWGAIMF
                     SGSKWYVIEPREIFVLDRIGGGDAFVGGLLYGILKGWEEEKWVQFGWACGAYVVTLLT
                     DYAQILDEEEIWSVWEGNVRVKR"
     gene            5794..6363
                     /locus_tag="PLF90_06720"
     CDS             5794..6363
                     /locus_tag="PLF90_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57005.1"
                     /translation="MKIRKAISKTIAERYRNSSKKEKTKILDEFTKITGYNRSYAIYL
                     LNRIGKKVVLWDKRKRLVFVGENIRVKKRKRPIIYDDKVVNWLKNIWVLLDYPCGKRL
                     SPYMEEIINRLDKNGMKIEEDIKEKLKGMSAATIDRKLRYYKKQLELKRRSKTKPGSL
                     LKSQIQVRTFSDWDEKMPGFIEVDLVDHK"
     gene            6350..6550
                     /locus_tag="PLF90_06725"
     CDS             6350..6550
                     /locus_tag="PLF90_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57006.1"
                     /translation="MTISEFINHHLLKFCEENKITFTKSRPYKKNDSCYVEQKNWSVV
                     RKAVGYWRYETKQEVETINQMR"
     gene            6814..7665
                     /locus_tag="PLF90_06730"
     CDS             6814..7665
                     /locus_tag="PLF90_06730"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK57007.1"
                     /translation="MIDYHVHIGKIFYGRNPLTPKKLIETMNKYGIEKSIILPIENPE
                     ETHFYLTTDYVLKICKKYREKLIPFCNVDPRRGKNNEKEERIEKIIEEYVKKGAKGFG
                     EVLANLQFNDKRMKNIYRACGDLKLSIVFHLGGVPGNSTIGLTDEIGLPYIEEVLNEF
                     PDTIFVAHGPGWWAEISGDVTAEERNSYPKGKIKKEGKVQYLLHNYPNMYADLSAGSG
                     YNALTRDIEYGIEFLNKFYNKLLFATDYLYPEQELPIIDYLKNLNITEEKKKKIFYEN
                     AKKLLNI"
     gene            7678..8517
                     /locus_tag="PLF90_06735"
     CDS             7678..8517
                     /locus_tag="PLF90_06735"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOK57008.1"
                     /translation="MDIRNFKVFILKSNEEIGKKSAEDAGEILENYTNKLNRKCLCVF
                     AAAPSQDTFLENLSKNKNIKWENIYAFHLDEYIDLPENHPNTFKEYLRIHIFEKVEIP
                     EKNVFYMKEAGKTEDEIIKNYTDLFIEKYQEIKRNESIYLAFIGIGVNGHIAFNEPGT
                     DLWNKEIITKIRIDDISVKQQYEDYKTHPNPEARYKSLDEVPRNALTMTVSAILLADK
                     IFCMVPGKQKGDAVKLTLEGEISNKVPASFLRLHKDVSLYIDIDSGSKLINKPETKIK
                     IGK"
     gene            8520..9398
                     /locus_tag="PLF90_06740"
     CDS             8520..9398
                     /locus_tag="PLF90_06740"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK57009.1"
                     /translation="MFIDIHVHSRKNPIMWKGKYYYISPENLIERYEKLGIEKGVLLP
                     GVSPECSIQPGSTHEMLEIYKMYPDRFIPFCNVDPRAVENSPLSPIDELLYIYKELGC
                     KGIGEVTANLPFTNPLFLNFFKCVEKVGFSLTFHISPQLGNFYGIYDEPGLPGLEYCL
                     NRFPDLIFIGHSQPFWAEIAELEKPYDRYGYPDYPVKKEGVVPKLMRKYKNLYGDLSA
                     GSGYNALARDLNYAVDFLNEFQDRLLFGTDICAPETPTPLVDLLLKLKEEKKINETVF
                     NKIARENAIKLLKLEG"
BASE COUNT         3815 a         1180 c         1701 g         2880 t
ORIGIN      
        1 gttcagtggc aagacagttt ggaagaaaat ggagattaac agaaacatat ggttgtactg
       61 gctgggattt ttcttttgca ggacataaag cactagggga ttggcagata gcactcggta
      121 taaatttaag gtgtcagcac ctttcctggt atacaatgct tggagaagca aaaagagatt
      181 atcccgcaag tattttctat caatctccat ggtggaatgc ttataaatat gtagaagatt
      241 attttgcaag aatacatctt gttatgacac agggagaaga agttagggac ttacttgtaa
      301 ttcatccaat tgaaagtatg tggactgttt ataaaatgcc tgactggaaa aatgaagaaa
      361 aaaaatggga atattcagac gaggtaaaaa aacttgatga aatgtttgtt aaactgtgtg
      421 atactttact ttccagtcat attgattttg attatggtga tgaagaaatt ttatcaagat
      481 tggcaaaaat tcagaaaaaa ggaaaccaaa caattttgaa agtaaacaag gcagaatata
      541 aaacaatttt agtaccccct ttatatacaa taagaagttc aacattagaa atttaaaaaa
      601 gaaggcggag aagttatatt tgtaaaagaa gtaccttctt ttgttgattg tgaaccttcc
      661 gatgaagtaa aaaaatttgc tgaaaattgt atagtagttg gttctataga agaaagtatt
      721 aaacatcttg agaaaataag aagaatatca ataaaagatg aaaatgggaa tgaaattaaa
      781 gaaatacttt atctattaag agaagatgaa aataattatt atctcttttt atgtaacaca
      841 agtatggaat gggacattaa tataggaaaa tactgtcttg taagagatag aaaaagagaa
      901 tttaaaaatg taattataaa gaactttcct gattggaaat acagacctat tgaacttaat
      961 ccacaaacag gagaaattta ttaccctgaa acaataaaag atgaaaatgg atataaaatt
     1021 aaaactgatt tttatgctct tagtagtcat ctttatgtaa tcccgaagaa aaaggaaatt
     1081 aatctggata taaaagaaac gaaaaaatat gaaatagtca gaaatttaaa aattgaaaag
     1141 gaaaaatggg aatatatttt aacagaaaaa aatgttcttg ttcttgatat gccttatttt
     1201 aaaattgaaa atggaaactg gcaggagaaa aaagaaattt taaaagttga tagagaagta
     1261 agaaggtttc tcggcttatc cccgagaggt ggagcgatgg ttcagccatg ggcaagaaag
     1321 aaaaataaaa atccaaaaag tgtaaaggtg agtttaaaat atgaatttca tatagaagaa
     1381 ttgccaaaag gagaaattaa aattgcatta gagaaaccag agttatatga aatttatata
     1441 aatggttata aactttcttc tgatatgaaa agtggctggt gggttgataa atcacttgaa
     1501 acaataaatt ttgctccatc aatcttaaaa ataggaaaaa atgagattat acttgaaaca
     1561 gattataacg agaatcatcc tggttttgaa attatatatt tactcggtga ttttggagta
     1621 aaactggtag agaatgattt aataataact ggtcttcctg aaactttgaa aacaggggac
     1681 tggacagaac agggattacc tttttattca ggaaatgttg gatatatatc tgtaattgaa
     1741 aaaattgatt gtgacgaaaa agaaaaaatt ttcctgaaaa ttccagaatt cagaggtgtt
     1801 ggaataagaa tttttataga tggaaaagaa gttggagtag tcgcctggcc cccatgtgaa
     1861 atagatataa ctgattatgt aaaaggaaaa gatagaatca atttaataat agaaatactc
     1921 ggacacagaa gaaactcaca tggaccatta cattattcag aaaaatggcc tgtatggaca
     1981 ggaccatatc agtttgtttc agaggggaat ttatggaaag ataaatatca gattgtgccc
     2041 tgtgggttaa tggaaaatcc tgaaataata ataaaaaagg agaaataaaa atgttcagaa
     2101 atttatcacc tgaagcaata aatataaaaa caaccattct tgaacgactg cgacttgcaa
     2161 aacttggtaa ctttgaagga atggatattt caatattaga atcttttgaa ttttcaagag
     2221 tagataaagt tgaaaaaatt aaaagcatgt ttgaaagttt tgatataaaa ataggtggat
     2281 ggtgtttacc ctttaacatt ggggaaacag aagaaagatt taatcagaat attgaaatgc
     2341 tgaatgaata ccttaaaatt actcaaacac ttggagcatt cagaatatac agttggatat
     2401 tacctttttc cgatgatctg ccttttaaag ataacttcca acttcattta cagagagcaa
     2461 aaaaagtttg tcagattatg gaaaaatata actgtaaact cggacttgaa tttataggaa
     2521 caaaaaaggt aagggaaaaa cacaaatatg aatttatctg gaatcttgag caaatgtgtg
     2581 aatttttaaa agaagtaagt atggataata ttggaatttt acttgacagt tggcatcttt
     2641 atgcttctga tggaaagatt gaagatataa aaaaattgaa agggaaggat ataatttatg
     2701 tacatgttaa tgatgcacct aatataccaa aagaaaattt aatagataat gaaagattct
     2761 tacctggaga aacaggagta attgatattg tggaattttt gaaagcactt aaagaaattg
     2821 aatatgatgg acctgtaaca cctgaaccat ttaataaaag agtaaatgaa ttgcctgatg
     2881 aaattgcagt gagattaaca ggtgggtatt tacttaaaat ttggaataaa gtctttaaat
     2941 gaaggttaat ttatgggata tatatacttt cctcaattta aaaatggtaa aaatggtctg
     3001 aaaatccatc ctggattaac aatacttgat tttgctcaaa agttaaaagt taaaataaat
     3061 gccgaatgcg gaggtatagg aaaatgtgga aagtgtgttg taagaattga gaaaggtgaa
     3121 gaaaatttaa atcaactgac tctttctgaa aaaaaatcaa atcttcctga aaaacacaga
     3181 ttagcatgtc aggcaagaat tgtcagagat gttttagata ttattgttta tataaaggaa
     3241 ttcggtgagt atgagatatt aaaatatgga atggaaagag aagttgaaat atgccctgtt
     3301 tatcaaaaaa aggaagataa agtttgtaaa gataacaaag agatagatgt ttacagaaat
     3361 gggatttttg ggcttgcagt agatattggt acaacaacaa ttgtttttga tatagttaat
     3421 cttgaaaatg gagatattat aggaaccata gcaaaaacaa atcctcaaat tatttttgga
     3481 aatgatgtta tttcaagaat agaatatgtt atggttgata aaaaagaaaa aaggttttta
     3541 aatccagatg aaagatatca aaaattacta gaacttcaaa aagtagttgt tgatttaata
     3601 aatgaatcaa taaaagaatt taacaatgat ataaagaatt caatttatca ggcagttatt
     3661 gttggaaatc ctacaatgag agacattttc ttccttcatg atatatcacc tcttggaaaa
     3721 actccttatg aaccattgat acttcaggga attacaaaaa aacctgaaga aatcggactt
     3781 gatataaata aaaatggaga aatttatgga ctgcctttaa ttggtggtca tgttggtgct
     3841 gatattgttg ctgatattct tgcttctgaa atttataatg aagaaaaagt ttgtatgatt
     3901 attgatatag gtacaaatgg agaaattgtt ataggaaata aagaaaaatt atttgccact
     3961 tcttgtgcag caggaggtgc ttttgaagga gttaatataa gttgtggaat aggaggaatt
     4021 gaaggagcaa taaaagaagt gaaaatttta aatggtaaag tttattattc aacaatagga
     4081 aataagtatc ctcttggaat atgtggttca ggtttgatag accttttaag tgaaatgctt
     4141 aaaaataaaa taattgatga gaaagcaagg tttagaaatg aaataaatga attttatata
     4201 actggagata taaaaataaa tcaacaggac atatttcaac tgataacctc aaaagcagca
     4261 ataagaactg cctggcaaat cctttcaaaa atttatccag ttgaaataga aaaaattgag
     4321 aaaatctatc tttcaggagg atttggaaat tttataaaca ttgaaagtgc tattaatatt
     4381 ggattaattc ctgatattga aagagaaaaa ataataaaaa taggaaacgg agcaattgaa
     4441 ggagcaagag aagttttatt atgttcagat aagagaaaac ttgcggaaga tattgccaga
     4501 aaagtgaagc atataaaaac aaatgaaata gaaaaggatt ttgatttcct tcttgcctca
     4561 aatatgtatt tttaatgact caccttttca ctcttacatt cccttcccat acactccata
     4621 tttcttcttc atcaagaatc tgagcataat cagtaagaag tgtaacgaca taagcaccac
     4681 atgcccagcc aaattgaacc catttttcct cttcccatcc cttcaaaatc ccgtataaaa
     4741 gcccaccgac aaaagcatcc cctccaccta ttctatcaag aacaaaaatc tctctgggtt
     4801 caatgacata ccattttgaa ccggaaaaca ttattgcacc ccataaatgc tgttgtgcac
     4861 ttacaacctc ccttaaagtt gttgcaaata cctcaacatt cggaaattgc ttttttgttc
     4921 tttcaatcat tcctttaaaa ttttcaatct gtgcctttat attttttcca ccaggttcag
     4981 gtccttcaat tccaagacat aactgaaaat cttcctcatt cccgacaagt atatctgtta
     5041 ctcttgcaat ttctgtaaat atctctctta attctttctc tctattcttc caaaatgatg
     5101 ctctgtaatt taaatcaaaa gaaatccttg ttccatattt ttttgctatt tttgatattt
     5161 caagacaaaa atttcctgtt tcaggcgata gagcagcaaa aagacctgac atatgaacaa
     5221 tctgaacacc ttcctttcca aatattcttt caaggtcaaa atcctctggt tttaaagtcc
     5281 ttccaacttc accggttcta tcattccaca ctcttggtgc tctacttcca taacctgtat
     5341 cagcaatatt gaattgatgc ctaaaacccc agggtccacc ctgtagaact tccttatatt
     5401 ccacatccat tcccctactt tttaaatttt ccttaataaa tcttgcaata ggacttcctt
     5461 taacaaaggc agtaagaacc ttaacaggca ttcccaaata tgaacttaca ctcgctacat
     5521 ttgtttctgc acttgttgcc tgcataatat aaagattgct tgtatgaaca ggttgcatat
     5581 tgaaaggagt aattcttaca cccatacttg taggaaccaa caaggcatat ttacaatctt
     5641 ttttcagttc cattatccct cctttttatt aagactattt tttattttac cacacttttt
     5701 tttcaatata aaattttcga attgcctcat taaaaaaatc caattgaaat tatacctcac
     5761 ctccgggaaa ataagaagaa aaaggagtta gatatgaaaa taaggaaagc gataagtaaa
     5821 acaatagcag aaagatatag aaattcaagc aaaaaagaaa agacaaaaat attagatgag
     5881 tttacaaaga ttacaggata taacaggagt tatgcgatat atttgttaaa caggataggg
     5941 aaaaaagtag tactctggga taaaaggaaa agattggtat ttgttggaga gaatataaga
     6001 gttaaaaaga ggaaaaggcc tataatttat gatgataaag tagtaaattg gttaaaaaat
     6061 atatgggtat tattagatta tccttgtgga aagagattgt caccttatat ggaagaaata
     6121 ataaacagat tggataaaaa cgggatgaag atagaagaag acataaaaga gaaattaaag
     6181 ggaatgagtg cagcgacaat agacaggaaa ttgagatatt ataaaaagca gttagagttg
     6241 aaaagaagga gtaagacaaa acctggaagt ttattaaaaa gtcagataca ggtaaggaca
     6301 ttttcagatt gggatgagaa gatgcctggg tttatagagg tagatttagt tgaccataag
     6361 tgaatttata aatcatcatc ttttaaaatt ttgtgaagaa aataagataa catttacaaa
     6421 atcaagacca tataagaaga atgatagttg ttatgtagaa caaaagaatt ggtcagtagt
     6481 aaggaaagca gttggatact ggagatatga aacaaaacaa gaagtagaga caataaatca
     6541 gatgagataa agaaaaaatt gatagaactt gaggataaat tgtttgagat ggtaaggaga
     6601 aaaagattga aagaaaaagg agaaaaaatt gaaaaagaag aaattttcgc ttagaatttt
     6661 atgtgaggca tcgtatataa attttactta gattcttata tgaggcaaca ggattatttt
     6721 cattagtcaa actattaata aaattttaag gtgaattttt acataaaatc cggtatgtta
     6781 tataattttc aaaaagtaaa aggaggaaat aaaatgattg attatcatgt tcatatagga
     6841 aagatatttt atggaagaaa tccattaaca ccaaaaaaac ttattgaaac aatgaataaa
     6901 tatggaatag aaaaaagtat tattttaccg attgaaaatc cggaagaaac acatttttat
     6961 ttaacaactg attatgtttt aaagatatgt aaaaaatata gagaaaaatt aattcctttc
     7021 tgtaatgttg acccgagaag aggtaaaaac aatgaaaaag aagagagaat agagaaaata
     7081 attgaagaat atgtaaaaaa aggagcaaag ggtttcggag aagtccttgc aaatttacaa
     7141 ttcaatgata aaaggatgaa aaatatttat agagcatgtg gagatttaaa actttcaatt
     7201 gtctttcatc ttggtggagt tccaggaaat tcaactatcg ggcttacaga tgaaatagga
     7261 ttaccttata tagaagaagt tttaaatgaa ttcccggata caatttttgt tgctcatgga
     7321 cctggatggt gggcagaaat ttcaggagat gtaacagcag aagaaagaaa ttcatatcca
     7381 aaaggaaaaa taaaaaaaga aggtaaagtt cagtatttac tacacaatta tccaaatatg
     7441 tatgctgacc tttctgctgg aagtggttac aatgcattaa caagggatat agaatacggt
     7501 attgaatttt taaataaatt ttataataaa cttttatttg caactgatta tttatatcct
     7561 gaacaggaac ttccaattat tgactatttg aaaaatttaa acataacaga agaaaagaag
     7621 aaaaagattt tttacgaaaa tgcaaaaaaa ttgttaaata tttaaaaagg agtaaagatg
     7681 gacataagaa attttaaagt ttttattctg aaaagcaatg aagaaattgg gaaaaaatca
     7741 gcagaagatg caggagaaat tcttgaaaat tatacaaata aattgaacag aaaatgtttg
     7801 tgtgtttttg ctgctgctcc ttctcaggat acatttcttg aaaatctatc aaaaaacaaa
     7861 aatataaagt gggaaaacat atatgcattt catcttgatg aatatataga ccttccagaa
     7921 aatcatccca atactttcaa ggagtattta agaatccata tttttgaaaa agtagaaatt
     7981 cctgaaaaaa atgtttttta tatgaaggag gcaggcaaaa cagaggatga aataataaaa
     8041 aattatacag atttatttat agaaaaatac caggaaatca agagaaatga aagtatttat
     8101 cttgcattta ttggaatagg agttaatgga catattgctt ttaatgaacc aggaactgac
     8161 ctgtggaata aagagataat aacaaaaata agaattgatg atatttctgt gaagcagcag
     8221 tatgaggatt ataaaactca tccaaatcca gaagcaaggt ataagagttt agacgaagtc
     8281 ccaaggaatg ctttaacaat gacagtttct gcaattttgc ttgcagataa aatcttctgt
     8341 atggttcctg gtaaacagaa aggtgatgct gtaaaattga ctcttgaagg tgaaatatca
     8401 aataaagttc ctgcttcctt tttaagatta cataaggatg tttctttata tatagatata
     8461 gattcaggtt caaaattgat aaataaaccg gaaacaaaaa taaaaatagg aaaataaaaa
     8521 tgtttataga tattcatgtt cattcaagga aaaacccgat tatgtggaaa ggtaaatatt
     8581 attatatatc accagaaaac ttaattgaaa gatatgaaaa acttggaata gaaaaaggag
     8641 ttctacttcc aggagttagt cctgaatgct ctatacagcc aggttcaacc catgaaatgc
     8701 ttgaaattta taaaatgtat cctgatagat ttataccttt ctgtaatgtt gacccgagag
     8761 cagtagagaa ttcaccttta tctccaatag atgaacttct ttatatttat aaagaactcg
     8821 gatgtaaagg aataggtgaa gttactgcaa acttaccttt taccaatcca ttatttttaa
     8881 atttttttaa atgtgtagaa aaggttggtt tttctttaac attccacata tctccacaac
     8941 ttggaaattt ctatggaata tatgatgaac ctggactccc cggccttgaa tactgtttaa
     9001 acagatttcc ggatttgatt tttattggac attcccagcc attctgggct gaaattgctg
     9061 aacttgaaaa accatatgat aggtacggct atccggatta tcctgtaaaa aaagaagggg
     9121 ttgtgccaaa acttatgaga aaatataaaa atttatatgg agacctttct gcaggtagtg
     9181 gttataatgc acttgcaaga gatttaaact atgcagttga ttttctgaat gaatttcagg
     9241 atagattact atttggaaca gatatatgtg cacctgaaac acctactccc ctcgttgatt
     9301 tacttttaaa gttaaaagaa gagaagaaaa taaacgaaac tgttttcaat aaaatagcaa
     9361 gagaaaatgc tataaaactt ttaaaactgg agggataaat gaaacttgca atttttggtg
     9421 gagaaaaagc aataaactca gattatagta aattttgtaa atggccaatt ataacaaaag
     9481 agatggaaga gaaagtcctt gatgttttga agaaaggagc aatgtcaaaa actgaattaa
     9541 caaaagaatt tgaaaagcaa tatgctgaac tttatg
//
LOCUS       DAOSAT010000069         9438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_4770_length_9438_cov_5.012256, whole genome shotgun sequence.
ACCESSION   DAOSAT010000069 DAOSAT010000000
VERSION     DAOSAT010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4770_length_9438_cov_5.012256"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PLF90_06745"
     CDS             complement(<1..773)
                     /locus_tag="PLF90_06745"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOK57010.1"
                     /translation="MKRNEIIKILKEKENIRDDQIEIVLKEKEKTGTPFGYLLIKFGL
                     LSAERWYNFALKELKCIPVQLSQMNIDKEILKMLPEFTCRKYRVIPIFRGNNKLVCAM
                     VDPVDEDVINEIKKISGMEIETRLVKEYEVKEIIETVISQGGIDVISPIEKKEMPEKV
                     FKPIRVREVSESSAVSVVEELVTKAMELKATDIHLEIEEEGLRLRYRINGLLYEFPPP
                     PLELYSSIVSHIKVLSNLDIAEKRVPQDGYFNMKLYGRDV"
     gene            complement(760..1227)
                     /locus_tag="PLF90_06750"
     CDS             complement(760..1227)
                     /locus_tag="PLF90_06750"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOK57011.1"
                     /translation="MRNLILIFIACLCFILSGGELIKAPDFVLNSIDGKEIKLSNYKD
                     KKVILNFWATLCPPCKEEIPYFVKFYNENKNKIEIIGIELTGKKKEIEQIVKKYNISY
                     PICISDGKIENLYGGIRFVPTTFVIDEKGYIILKKVGLMTEEELKGIIKNEKK"
     gene            complement(1249..1446)
                     /locus_tag="PLF90_06755"
     CDS             complement(1249..1446)
                     /locus_tag="PLF90_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57012.1"
                     /translation="MKCPLRLRIQKRPLGEAAFGVPEQVEDFDMCIEENCAWWDEKNK
                     SCLVRHLISIDEKLEILKSKI"
     gene            complement(1540..2889)
                     /locus_tag="PLF90_06760"
     CDS             complement(1540..2889)
                     /locus_tag="PLF90_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOK57013.1"
                     /translation="MEINRKIFREYDIRGIYEDDLKGDLPYYIGKAFGSYIKRQNIGN
                     VCVGGDNRFTTPEIKEKLIKGLIDTGCEVVDIGIIPTPLLYFSVHFYKYGSGIMVTAS
                     HNPPQFNGFKMVVGNKSLYGKEIQKIADIIEKEDFEKGNGKLDKKDVIDEYIKFMVEK
                     FKFNKKLKVGVDTGNGTVGPLIEKLFRKLNVEFVGLYLESDGNFPNHLPDPVVPENLK
                     DLIEIVKENNLDCGFGFDGDGDRLAVVDENGDILWGDKLMIVYSKDILIKNKGAKIIF
                     DVKCSKSLEEVIEKLGGKPVMWKTGHSLIENKLHEEKSPLAGELSGHLYFADEYFGYD
                     DAIYACLRLLRIMDKENKKLSEFFKDVKKYYSTPEIRIEVSDEEKFEIVEKVKKFYAG
                     KYRINDIDGVKVYYPSGWALLRASNTQPALVVRIEGETEEDLNKIKREFIEKIGNFK"
     gene            2965..3690
                     /locus_tag="PLF90_06765"
     CDS             2965..3690
                     /locus_tag="PLF90_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOK57014.1"
                     /translation="MKKGYVLTELIVGLVIFILLATGIFLGVKNVMDRAKVMSAKVQI
                     SQLALLLEQIKNDTGYYPAFLSDLTLNSPPKLQEKGWNGPYTNKIPFDPWGNPYFYKI
                     PPTTVFSSPKLPRVHGKPDTYTANFDAIEGKGRIRVENYGVTSCSIYINGVEVVKENE
                     FRNKPKPQIIEKEVTLKDENNTLTWARSTPGDFLYFSIIVDNMPTGEYFIVGSYGKDG
                     KESGKGYNKDIVWVSNKYPNFQE"
     gene            3701..4144
                     /locus_tag="PLF90_06770"
     CDS             3701..4144
                     /locus_tag="PLF90_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57015.1"
                     /translation="MKKNRRKKYLINKPVQFIFSGITIYFIIIAIILIGGLTYFITLN
                     TILSQLELEKKIINAYEIIKNINILLLKRIGILMLLLIFLTFYLEIRFLHRIVGPLYR
                     IEKSLNEIIEGKSVEYIKLRKKDFLKPFAETFNKLIDYINTIKKS"
     gene            complement(4141..4479)
                     /gene="rsfS"
                     /locus_tag="PLF90_06775"
     CDS             complement(4141..4479)
                     /gene="rsfS"
                     /locus_tag="PLF90_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOK57016.1"
                     /translation="MSDSENLKRIKKIVKLINDKKGENVVVLDLKNLTWITDYFIIAS
                     GESLIQTKAIAENIIENLKETPVSVEGIDDGRWILVDYGEIIVHIFLIETRNYYKLEK
                     LWAEARIVEI"
     gene            complement(4502..5461)
                     /locus_tag="PLF90_06780"
     CDS             complement(4502..5461)
                     /locus_tag="PLF90_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK57017.1"
                     /translation="MVRKVKVGIIGAGGRANFQAKSIIGSQIGEVKIVYSPFLEEAKS
                     FSEKYGIEYTDSLDDVLNNPDIDAITVSTPNATHYEIAKKGLESNKNVLVEYPPTLKL
                     EQLEELINLAKEKNLVYWVSLTQLLENPHYTIKKNLNLIEKPLSYNFSYISSFLGGWY
                     SQISLSGPVYLWQHFHFVSQLLDIWKDVEEVSVFENIEYSSEGIMSLTFSVMNIKFKS
                     GIISTIEFGMGIKDVSDTRIKLVGDGGIFYYEKGKLYFINKKEGKREIEMEKIDIGVD
                     TVNFLKKVQEGKFNIEKALEAREILKICLCAEISAKEKRIVKM"
     gene            complement(5505..7295)
                     /gene="mnmG"
                     /locus_tag="PLF90_06785"
     CDS             complement(5505..7295)
                     /gene="mnmG"
                     /locus_tag="PLF90_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOK57018.1"
                     /translation="MKKYDIIVIGGGHAGIESVMVCKKMGMEVLLITFEKEKIGYMSC
                     NPAIGGVGKGQIVKEIDALGGQMAKATDKTGIQFRTLNTSKGPAVWSSRAQVDRRKYN
                     EYMKKLIEENVEILEGEVTDIIVKNKSVLGVEINGEEFISSKCVILTPGTFLNGVIHI
                     GFESFSAGRIEDKKVSKKLSDKLKELGFEIMRFKTGTCARLDGSTIDFSSLTPQYGDE
                     KIRPFSFSTDKIEIEQLPCYITYTNEKTHQIIKKNLDRSPLFTGIIKGTGVRYCPSIE
                     DKVVKFPHHQRHHIFLEPEGKDVNIYYPNGISTSLPVDVQDEFIHTIKGLENVKILRY
                     GYGIEHDVVNPLNIYPTLETKLVENLFLAGQINGTTGYEEAAGQGLIAGINASLKVKN
                     ERPLILDRTTSYIGVLIDDLTTKGTNEPYRMFTSRVEYRLMIREDNADIRLREIGYKI
                     GLVSKEEWERTRKKIEDIKKIKEILKSRKINVDGKNISLFEYVRRHEVKLTDFVSDFK
                     EDTLFTAEVEIKYAPYIERNLREIEEFKNLERIKIPSDIDYNKIPGLSLEIKEKLSKL
                     KPLNLGQASRISGITPSAISILMVYLKKFS"
     gene            complement(7314..8357)
                     /locus_tag="PLF90_06790"
     CDS             complement(7314..8357)
                     /locus_tag="PLF90_06790"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOK57019.1"
                     /translation="MVNVYFLPFEKIDKLDDFLNRFDFLKNFSEKDKIGLKIHFGNSH
                     HNNNVSPDFVLPIVNKLKKKNILGILTDTNVLYRGERDDTFSHFEVVYKKGYDKLGVP
                     ILIAGGFDADYEFDIDINGKHFKKLYYAKEYEKFDGMIAITHFKGHGLSGIGGTLKNI
                     GMGCASRKGKFAMHSNISPVVNNSICKGCGKCVENCLFNAITLKNEKAFIDREKCKGC
                     AFCIHVCPFGAINIPWSSVTTEEFQERIVEYAYGIIKFYKDKFLAINFLTNIAGDCDC
                     CSNPGEILSKDIGVCVSDDPVAVDKCSSDLIREINKKDVFLEIKPKVDYTTQFRYAEK
                     IGLGTTKYKLIVF"
     gene            complement(8394..8609)
                     /locus_tag="PLF90_06795"
     CDS             complement(8394..8609)
                     /locus_tag="PLF90_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOK57020.1"
                     /translation="MAECLNLERNLKNCSCSYKSCSKRGVCCECLSSHWSRKELPGCL
                     FPPEAEKTYNRSIEYFIKVWSEKLNLK"
     gene            complement(8711..9274)
                     /locus_tag="PLF90_06800"
     CDS             complement(8711..9274)
                     /locus_tag="PLF90_06800"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOK57021.1"
                     /translation="MVNASELREGMIIKREGKLYKVIEAETKARTAQFSSYTHLKLQD
                     LKTGHVHDLRLSPDEKIENVDIEEIEMEYSYADGDNFYFIHPETFEIIELPGYTIGDF
                     KRYMKEGVKLKIQVYEGNPVSVVIPEFVDLKVVTTGEGIKGGTDSTWKSATLENGMEI
                     LVPQFIKEGDIVRVSTKTNQYLERVHK"
BASE COUNT         3086 a         1726 c         1050 g         3576 t
ORIGIN      
        1 acatctcttc catataattt catattaaaa taaccatcct gaggaaccct tttttctgct
       61 atatcaagat ttgaaagtac ttttatatga gaaacaattg aagaataaag ttcaagagga
      121 ggaggtggaa attcataaag aagcccgttt attctgtatc tcaatctcaa accttcttct
      181 tctatttcaa gatgaatatc tgttgctttc aattccattg cttttgtaac aagttcttca
      241 acaacactaa cagctgacga ttctgaaact tcccttactc ttattggctt aaatactttt
      301 tcaggcattt cttttttttc tataggagaa ataacatcaa ttccaccctg tgatattact
      361 gtttctatta tttccttcac ttcatattct ttaaccaatc ttgtttcaat ttccattcct
      421 gatatctttt ttatttcatt tataacatct tcatctaccg ggtcaaccat tgcacagaca
      481 agtttattat ttcccctaaa tattggaatg actctatatt ttctgcatgt aaattcagga
      541 agcattttta aaatttcttt atctatattc atctgactta actgaacagg aatacattta
      601 agttctttta aagcaaaatt ataccatctt tcagcagata aaagtccaaa tttaatgagt
      661 aaataaccaa aaggagtacc tgttttttct ttttccttca atactatttc tatctggtca
      721 tccctgatat tttctttttc ctttaaaatt tttattattt catttctttt catttttaat
      781 aatccctttc aattcctctt cggtcatcaa acccactttc tttaaaatta tataaccttt
      841 ttcatctatt acaaatgtag ttggaacaaa tctaatacct ccgtataaat tttctatctt
      901 tccatcactt atacaaatgg gataactgat attatatttc ttcacaatct gttcaatttc
      961 ttttttcttc cctgttagtt caattccaat aatctcaatt ttatttttgt tttcattata
     1021 gaatttaaca aaatagggaa tttcctcttt gcatggagga caaagagtag cccagaaatt
     1081 caaaataact tttttatcct tataattgga aagttttatt tctttaccat caattgaatt
     1141 taaaacaaaa tcaggtgctt taattaattc acctcctgat agaataaaac acaaacatgc
     1201 tataaaaatt aaaattagat ttctcatcgt ttctctcctt taaatttttt aaattttact
     1261 ctttaaaatt tcaagttttt catctattga aatcagatgt cttaccaaac aacttttatt
     1321 tttctcatcc caccacgcac aattttcttc tatgcacata tcaaaatctt ccacctgctc
     1381 aggcactcca aatgctgctt ctccaagagg tcttttctga attctcaatc tcaacggaca
     1441 tttcattttt catcccccct ttctcccacc tttaaggtgg gcttggaaaa taattgacgg
     1501 acaagggttt tcaactgtca tttttagtct tttaaaaaat tatttaaaat ttccaatttt
     1561 ttcaataaat tctcttttta ttttattaag gtcttcctct gtttctcctt caattctaac
     1621 cacaagtgct ggctgggtat ttgatgctct cagtaatgcc catccagagg gataataaac
     1681 cttaacacca tcaatatcgt ttattctata ttttcccgca tagaactttt ttaccttttc
     1741 aactatttca aatttttctt catcagaaac ttcaattctt atttcaggtg ttgaataata
     1801 tttttttaca tctttgaaaa attcagatag tttcttattc tctttatcca taatccttaa
     1861 taatctcagg caagcatata ttgcatcatc ataaccgaaa tattcatcgg caaagtataa
     1921 atgccctgaa agttctcctg caagaggtga tttttcttca tgaagtttgt tttcaattaa
     1981 agaatgaccc gttttccaca taaccggttt tcctccgagt ttttctatca cttcttcaag
     2041 ggatttggaa catttaacat caaaaataat ctttgcacct ttgtttttaa taagaatatc
     2101 ttttgaatat actatcatta atttatcacc ccagagaata tctccatttt catcaactac
     2161 tgcaagacgg tctccatctc catcaaatcc aaaaccacag tcaagattat tttctttaac
     2221 tatttctatc aggtccttta aattttcagg aactacaggg tcaggaagat gatttggaaa
     2281 attaccatcg ctttcaagat ataatccaac aaattcaaca ttaagttttc tgaacaattt
     2341 ctctatcaaa ggacctactg ttccatttcc tgtatccaca ccaactttta gttttttatt
     2401 aaatttaaat ttttcaacca taaattttat atattcatca atcacatctt ttttatctaa
     2461 ctttccattc cctttttcaa aatcctcttt ttcaattata tctgctattt tttgaatttc
     2521 ctttccatac aaacttttat ttcctacaac cattttaaaa ccattgaatt gaggtggatt
     2581 atggcttgca gtaaccataa tacctgaacc atatttataa aagtggacag aaaaatataa
     2641 aagaggagtt ggaataattc ctatatcaac tacttcacag ccagtatcaa taagaccttt
     2701 tatcaatttt tctttaatct caggagttgt aaatctgtta tcaccaccaa cacaaacatt
     2761 tccaatgttt tgccttttaa tataagaacc aaatgctttt ccaatgtagt aaggtaagtc
     2821 cccttttaag tcatcttcat aaatacccct tatatcatat tccctgaata tttttctgtt
     2881 tatttccata taaaaaatta tacaaaaaaa attgaaaatt gacaataaag aataaaagta
     2941 ggtaaaatta gttaggaggg aaaattgaaa aaaggatatg ttttaacgga actgattgta
     3001 ggtttagtta tatttatttt acttgcaaca ggtatttttt taggtgttaa aaatgttatg
     3061 gatagagcaa aggttatgag tgcaaaagta cagatttcac aacttgctct tttacttgaa
     3121 cagataaaaa atgatactgg atattaccct gcatttctct ctgatttaac actcaattca
     3181 cctccaaaat tacaggaaaa aggatggaat ggtccatata caaacaaaat tccttttgac
     3241 ccatggggaa atccatattt ctacaaaatc cctcctacaa ccgtcttttc ttctccaaaa
     3301 ttaccaaggg ttcatggaaa accggatact tatacagcaa attttgatgc aatagaagga
     3361 aaaggtagaa taagagttga aaattacggt gtaacttcct gctcaattta tataaatgga
     3421 gttgaagttg taaaagaaaa tgaatttaga aataaaccca aaccacagat aatagaaaag
     3481 gaagtaacac ttaaagatga gaataatact ttgacatggg caaggagtac acctggagat
     3541 tttttatatt tcagtataat tgttgataat atgccaacag gtgaatattt tatagttgga
     3601 agttatggta aagatgggaa agaaagtgga aaaggataca ataaggatat tgtttgggtt
     3661 tcaaataaat atccaaattt tcaggaataa gaggtgaaaa atgaaaaaaa atagaaggaa
     3721 gaagtatctt ataaacaaac ctgtccagtt tatttttagt ggtattacaa tttattttat
     3781 cataatagct ataatcctta ttggaggcct tacatacttt atcactttaa atacaatttt
     3841 atctcaactt gaacttgaaa agaagattat aaatgcatat gaaataatta aaaatattaa
     3901 tattcttctt ttgaaaagaa ttggaattct tatgttattg cttatatttc ttacttttta
     3961 ccttgaaata agatttttac acagaatcgt tggcccatta tatagaattg aaaaatcttt
     4021 aaatgaaatt atagaaggta aaagtgttga atatataaaa ttaagaaaga aggacttttt
     4081 gaaacctttt gctgaaacat ttaacaaatt aatagattat ataaatacaa taaaaaaatc
     4141 ttaaatttca actatccttg cttctgccca aagtttttca agtttataat aatttcttgt
     4201 ttcaattaaa aaaatatgta ctataatttc tccataatct acaagtatcc atctgccatc
     4261 gtctatccct tcaacagaaa caggtgtttc ttttaagttt tcaataatat tttctgctat
     4321 tgcttttgtc tgtattaatg actcccctga tgcaataata aagtaatctg taatccatgt
     4381 aagatttttt aaatcaagaa caactacatt ttcacccttt ttatcattta ttagttttac
     4441 tatctttttt attcttttca gattttcact atcgctcata taaaaattat accttttaaa
     4501 attacatttt aacaattctt ttttctttcg ctgatatttc tgcacacaga catattttta
     4561 atatttctct tgcttccaat gccttttcta tgttaaattt accttcctga acttttttta
     4621 gaaaattaac agtatcaact ccaatatcaa ttttttccat ttcaatttct ctttttcctt
     4681 cctttttatt tataaaataa agttttccct tttcataata aaaaatccct ccatcaccta
     4741 caagttttat ccttgtatca gagacatctt ttattcccat tccaaattca attgtagata
     4801 taatgccaga tttaaattta atattcatta ctgaaaaagt taaagacata attccttcag
     4861 atgaatattc aatattttca aaaacactca cttcttccac atctttccat atatcaagta
     4921 attgacttac aaaatgaaaa tgttgccaga gatatacagg acctgacaga gaaatttgag
     4981 aataccagcc acctaaaaag gaagaaatat aggaaaaatt gtaagatagg ggcttttcta
     5041 ttaaatttaa atttttcttt attgtataat gaggattttc aagaagttgg gttaaactta
     5101 cccagtaaac aagatttttt tcttttgcta aatttataag ttcctcaagt tgttcaagtt
     5161 ttagagttgg tggatattct acaagaacat ttttgttact ttcaagcccc tttttagcaa
     5221 tttcatagtg agtcgcattt ggagtagaaa ctgttattgc atcaatgtct ggattattta
     5281 acacatcatc aagtgaatct gtatattcta tcccgtattt ttctgaaaaa ctttttgctt
     5341 cttcaagaaa aggagaataa acaatcttaa cttctcctat ctgacttcca ataattgatt
     5401 ttgcctgaaa atttgctctt ccacctgctc caattattcc aactttaact tttctcacca
     5461 taaacctcct ttttgtcaaa tattaaaata tcacaaattt aaattcaaga aaattttttt
     5521 aggtaaacca taagaattga aattgcagaa ggagttattc ctgaaattct gcttgcctga
     5581 ccgagattta atggttttaa ttttgacaat ttttctttta tttcaagaga aagacctgga
     5641 attttattat agtcaatatc agacgggatt tttattcttt caagattttt gaactcctct
     5701 atttctctta aattcctttc aatataagga gcatatttta tttcaacctc tgcagtaaaa
     5761 agagtatcct ccttgaaatc gcttacaaaa tctgtaagtt taacctcatg ccttcttaca
     5821 tattcaaaaa gggatatatt tttaccatca acattaattt ttctactttt caatatttct
     5881 ttaatttttt taatatcttc aatttttttt cttgtccttt cccattcctc tttacttaca
     5941 agtccaattt tatatcctat ctccctcaat cttatatctg cattatcctc ccttatcatc
     6001 aatctatatt caacccttga agtaaacatc ctgtatggtt catttgtccc ttttgttgtc
     6061 aaatcatcta ttaaaacacc tatataactt gttgttctat caagaattaa aggtcgctca
     6121 tttttaactt ttaaagaagc atttataccc gctatcaatc cctgaccagc cgcctcttca
     6181 taacctgttg ttccatttat ctgacccgct aaaaataaat tctcaaccag ttttgtctca
     6241 agtgtcggat atatattcag aggatttaca acatcatgtt caatgccata accatatctt
     6301 aaaattttaa cattctcaag cccttttata gtatgaataa actcatcctg aacatcaaca
     6361 ggtaaacttg tagaaattcc gtttggataa taaatattta catctttccc ttcgggctct
     6421 aaaaatatat gatgtctctg atgatgagga aattttacaa ctttatcttc aattgaagga
     6481 caatacctta ctcctgtacc tttgattatc cctgtaaata aaggacttct atcaaggttc
     6541 ttttttataa tctgatgtgt tttctcattt gtataggtaa tgtaacaggg aagttgttca
     6601 atttcaattt tatcggttga aaaagaaaat ggtctgattt tttcatctcc atattgtgga
     6661 gttagagaag aaaaatcaat tgtactacca tcaagacgag cacatgttcc tgttttaaat
     6721 ctcataattt caaatccaag ttctttaagt ttatcagata atttcttact tactttttta
     6781 tcttcaattc ttcctgctga aaaactttca aaacctatat gaataacacc atttaaaaaa
     6841 gtaccaggtg taaggattac acattttgaa gatataaact cctcgccatt tatttcaact
     6901 cctaaaacag atttattttt cacgattatg tctgtaacct ctccttcaag tatttcaaca
     6961 ttttcttcaa ttaatttttt catatattca ttatattttc ttctatcaac ctgtgctcta
     7021 cttgaccata ccgcaggacc ttttgaagta tttaatgttc tgaattgtat acctgtttta
     7081 tcagttgctt ttgccatttg accaccaaga gcatcaattt ctttaactat ctgacctttg
     7141 ccaactccac caatagcagg attacaggac atgtagccaa tcttttcttt ttcaaatgtg
     7201 ataagtaaaa cctccatccc catcttttta cagaccataa cactttcaat accagcatgt
     7261 cctccaccaa ttacgataat gtcatatttt ttcattatta atgaaaggaa attttaaaat
     7321 acaatcaatt tatattttgt tgtaccgagt ccaatttttt ctgcatatct aaattgtgta
     7381 gtataatcta ctttcggctt tatttctaaa aatacatcct ttttatttat ttctcttatt
     7441 aaatcagaag aacatttatc aactgcaacc ggatcatcag aaacacatac acctatatct
     7501 tttgataata tttctcctgg atttgaacag caatcacaat ctccagcaat atttgttaaa
     7561 aaatttatcg ccagaaattt atccttataa aattttatta ttccataggc atactcaaca
     7621 attctttcct gaaattcctc cgttgttaca cttgaccatg gaatatttat cgcaccaaag
     7681 ggacacacat gaatacagaa agcacatccc ttacattttt ctctatcaat aaatgctttt
     7741 tcattcttca gagttatggc attgaataaa caattctcaa cgcatttccc acatccttta
     7801 caaatagagt tattaacaac aggagatata tttgaatgca ttgcaaattt accttttctt
     7861 gaagcacaac ccattccaat atttttcagt gtcccgccta ttcctgaaag tccatgtcct
     7921 ttaaaatgtg ttattgcaat cataccatca aatttttcat attcctttgc ataatagagt
     7981 tttttaaaat gtttaccgtt tatatcaata tcaaattcat aatcagcatc aaaaccacct
     8041 gcaattaaaa taggaacacc gagtttgtca taacccttct tataaacaac ttcaaaatgg
     8101 gaaaaagtat catccctctc gcctctgtat aaaacatttg tatctgtaag aatacctaaa
     8161 atatttttct tcttcaattt attaacaatt ggtaaaacaa aatcaggaga aacattatta
     8221 ttatgatgtg aatttccaaa atgaatttta agaccaattt tatccttttc actgaaattt
     8281 tttaaaaaat caaaacgatt caaaaaatca tcaagtttat ctattttttc aaaaggtaaa
     8341 aaatacacat ttaccatttt tttatacctc ctgtttcaga aatttttttg actttacttt
     8401 agattaagtt tttcgctcca aacttttata aaatactcaa ttgacctgtt atatgttttc
     8461 tctgcttccg gtggaaaaag gcatccaggc agttcctttc ttgaccagtg agaacttaaa
     8521 cattcacagc atacacctct ttttgaacaa cttttataag aacaggaaca attctttaaa
     8581 tttctttcta aatttaaaca ttcagccatt ttgtcctcct tttttggtaa cttttaaaaa
     8641 ttatacaaca attttaaaaa attgaaagga tacatttatt ttataaaaat caatgaaaaa
     8701 agcgaaaaat ttatttatgg actctttcaa gatattgatt tgtctttgtt gaaaccctta
     8761 caatatctcc ttcctttata aattgaggaa ctagtatttc cataccattt tcaagagttg
     8821 cacttttcca tgtactgtct gttcctcctt ttatcccttc ccctgttgtt accactttta
     8881 aatcaacaaa ttcaggaatt acaacactta caggattacc ctcataaacc tgaattttca
     8941 gtttaacccc ctctttcata tatcttttaa aatcaccaat tgtatatcca ggaagttcaa
     9001 taatctcaaa tgtttcagga tgtataaagt aaaaattatc tccatctgca taactgtatt
     9061 ccatttcaat ctcctcaata tcaacattct ctattttttc atcaggtgat aatcttaaat
     9121 cgtgaacatg tcctgttttc aaatcctgaa gtttaagatg tgtataactt gaaaactggg
     9181 ctgttctggc ttttgtctct gcttcaataa ccttatacaa tttcccctct ctttttatta
     9241 tcattccttc tctcaattca gatgcattca ccatctttgc ccccttttta ccatttctat
     9301 tttatcctaa atttttaatc ttttcaaaaa atatattttt aaatttgaca tatcatttaa
     9361 gttacgatag attgcagaaa gataaaaagg aggaaagatg gaaaagttta ttataagcag
     9421 ggatgatagt atttatga
//
LOCUS       DAOSAT010000070         9407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_4792_length_9407_cov_5.178144, whole genome shotgun sequence.
ACCESSION   DAOSAT010000070 DAOSAT010000000
VERSION     DAOSAT010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4792_length_9407_cov_5.178144"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLF90_06805"
     CDS             complement(<1..421)
                     /locus_tag="PLF90_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOK57022.1"
                     /translation="MRNLLFNLCKIKNARVKRISSYDMEGRNKDAWTISPGETKTIAE
                     IDGPGCITHIWMTQGGPLFLYREVILKFYWDGEENPSILVPLGDFFCLGHSLVNSFCS
                     LPFTASTANPYKFGAGCALNCYLPMPFRKHAKIEITNE"
     gene            complement(503..688)
                     /locus_tag="PLF90_06810"
     CDS             complement(503..688)
                     /locus_tag="PLF90_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57023.1"
                     /translation="MEEGNKRVQEILEKIDERMERMDKDHKEMLERMDDALRFIGTLI
                     NCSLIPPLRWDWRIVDD"
     gene            1034..2896
                     /locus_tag="PLF90_06815"
     CDS             1034..2896
                     /locus_tag="PLF90_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57024.1"
                     /translation="MRALIIGAILCFIIGTADIYNLVKIQGSYMTLDFTTGFAIFFIF
                     FITLFNYLFKKIFKKEGFKTEELIIIYIMMIVSCSIPTMGLTLYLVPLIAGVKYYSNP
                     QNEWDTIILPHLKRNLIVQDEKAITWFFEGIPKGEKLPWGPWIKPLSIWIFLILFIYL
                     AMIFIMIILHKQWSENEKLNYPMTKAPIELINSENTNIFKNGLFWFGFLIPFIFGLIN
                     GLHFYFPNIPPLTLVRNVPIFRKTLNLHFRISFPMIGFTYLVNLPLAFSLWFFCLLTT
                     IEQGFFNITGFGSQEFLPYNADRPLLGWQSLGSLIVIVLYGLWISRKNLINIIRTKEE
                     DEIISPKISFFGLIFSLLFIYFWLLYSGISPLPAILFIFFAFLIYIGITRVICEGGLA
                     ATRAPVISPVIINSFLGSTRLGPSNLIGLGLTFVYSSDVRTFVMASVANGLKMAENIK
                     KKKRLIFWAIIISIFITLFSSIWATIILCYKYGGINANQWFFVAGPQYPLKYVADKIK
                     NPKGPDWQYIGFTGIGAFFTVFLFIMRLRFLNFPFHPLGFAFSTIMMTNALWFSIFIS
                     WLIKVLILRYGGAKIYEKFKGLFIGLIIGQFVISGLFIIVDLITGKTGNTLFWA"
     gene            2947..3429
                     /gene="amaP"
                     /locus_tag="PLF90_06820"
     CDS             2947..3429
                     /gene="amaP"
                     /locus_tag="PLF90_06820"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HOK57025.1"
                     /translation="MKIWSRIVGTIYILMGIFFVFCLLSFFSNENLCESFINFIKNNL
                     YKVEIFATIVLIIGIIYVVNWIDYIYRTKAISFDNPSGKVRVSLKAIKDSITTTILKE
                     IDGIKTIKVKTFVTPKGLETKINLKLFSNLNIPDICSNIQELKKIIFKTQLELKGYQI
                     "
     gene            3447..3848
                     /locus_tag="PLF90_06825"
     CDS             3447..3848
                     /locus_tag="PLF90_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOK57026.1"
                     /translation="MISEIETHMEVLNSLLKFNLFEKLIEISELIVKSLKKGNKILLC
                     GNSGSAADCQHFAGEMVNRYKKKRRPLPFISLTTDTSIITSIGNDYKFEEIFSKQVMA
                     LGKENDILICFSTSGESKNVIEAAKVAKKLK"
     gene            4063..4350
                     /locus_tag="PLF90_06830"
                     /pseudo
     CDS             4063..4350
                     /locus_tag="PLF90_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
     gene            complement(4408..4545)
                     /locus_tag="PLF90_06835"
     CDS             complement(4408..4545)
                     /locus_tag="PLF90_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57027.1"
                     /translation="MKLVFIGGAHRHLGIIRAVLDDEAISSLCEICLYDLNKVVPLSH
                     F"
     gene            4838..6814
                     /locus_tag="PLF90_06840"
     CDS             4838..6814
                     /locus_tag="PLF90_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOK57028.1"
                     /translation="MELKKWFPKRAIHLDFHTMPGVYDVGIDFNGKEFVKTLKEANVD
                     YITTFAKCNLGFAYYPTKIGIIHPGLKKRDLLGEIIEECHKEGIMVSAYFNVGLDHEN
                     SVRHRDWCKVNKEGQIAEMQYMGHFFRKMCLNTDFKDYILGMIEEVIDNYPVDGIFLD
                     CFTLSPCYGGECVREMKKLNIDPSDEIKARDFCWMITEKFMEEVKKILKRKNKDIFLF
                     FNGLPYRKQPTHLELEVLPTGGWGYDFLPWIIRYARTLKKPFFTMTGRFHKGWGDFGG
                     LRTFHSLMFDLYYSVSNGGTCSIGDHMHPRGKLEKEVYDLIGKCYSEIKKIEEFTENA
                     ENITEMVIIEPNLSMFYGYHFDYSSLAGATRMLSELKYQFDVSDGTDDISKYKIVILP
                     DNIKVDKNLREKLKKHLKKGGILISSDLSCFDLEKKKFIFDDYKIEFEGEEENNPTYF
                     VGEKEFSDGIPDMPLTIYDQGISIKAKKGSKIIAYIYKPYFNYKSWDWEHENLYTPPE
                     KNTGRPAIVQFGNIIHFSFPVFKNYHNHAYIYYKKLLYNCLEKIYPEPLIKHKNIPSF
                     VQITLASQKNKNIIHILAYIPELRGKSMQIIEEPLILKDIEIGVKNIDKKKIKRIYLV
                     PSNEEIEFKEEGNYIWFKIESMAGYQMVVIEKMD"
     gene            6819..7847
                     /locus_tag="PLF90_06845"
     CDS             6819..7847
                     /locus_tag="PLF90_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOK57029.1"
                     /translation="MKAAITEGKGDVKIKEVSIPEINDYQCLCKTLACATCTGTDLKI
                     IDGKMFWCKNYPVILGHESVGVVIKKGKKVRYIKEGEIFLRTTCVYPGEKIDLYWSAI
                     GGFSEYGKITDIKAMKEDNKEKEINHYTVYQQKIPEELNISPFDATIFITLKETASFL
                     LNIGCKFENSLLILGSGPVGMSLVYFSKLIGCFPVIVAGRNSKTLEHIKKIGADFIIN
                     TENEKIEKVKEITNGKGVDFIIDTTGNTEFVLSVINLLSENGKIAPYASYSEPSPFKK
                     YEKTGKFILKGPSEPITHNYILKLTEMNILKPSLFYSHILPFEEIEYGFQLIRKKEAF
                     KIVFKMEV"
     gene            7849..8835
                     /locus_tag="PLF90_06850"
     CDS             7849..8835
                     /locus_tag="PLF90_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOK57030.1"
                     /translation="MKIKALIYQEANKFEISELTLDEMKDEDIKVKTIISAISPGTER
                     WVLKGKHIGTKFPCVPGYHRIGIIEKVGKNVKNFKEGDIVYGIDNRWKENIYRMWGAH
                     VSHSVGNWKNYWFISSKIPDNFELETIVFTQLSAVATRGIKALEVKSREKIAIIGAGF
                     LGLCACQLSIYRNAVPILIDKDPERVEFGKKFTQFSFLIDDKEIDEKLKEIAPEGFDH
                     LYDTVGDPNTTDKLVQITKFRGKILLQAQYFDKEKCAIDLDQIKIREITIKTTIGIDT
                     IDFYETLDYIKKRILKISEMITHRFTSEELTKGFEILRDGKPFNLGILFWWK"
     gene            8957..>9407
                     /locus_tag="PLF90_06855"
     CDS             8957..>9407
                     /locus_tag="PLF90_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOK57031.1"
                     /translation="MRYENMFPEQIREAIKKNIPIVLPVGVIEYHSEHLCIGVDTLLV
                     LKALEEIEKEMEIIILPPFYYGCASYAVEPPEGNGTIHIKPEVIYKFAKNLFENLLRV
                     GFRNIYVIIHHQSENFIAGMPTDLAFKLAARESIFEFLEKRYGEGWWG"
BASE COUNT         3549 a         1197 c         1496 g         3165 t
ORIGIN      
        1 cttcatttgt tatttctatt tttgcatgtt ttctgaaagg catgggtaga taacagttta
       61 aagcacatcc agcaccgaat ttatatggat ttgcagttga agcagtaaag ggtaaagaac
      121 agaaagaatt aacaagagaa tgcccaaggc agaaaaaatc accaagaggt acaagaatac
      181 ttggattttc ttctccatcc cagtaaaatt ttaaaattac ttctctatat aaaaataaag
      241 gtcctccctg agtcatccag atatgagtta tacatccagg cccatcaatt tctgcaattg
      301 tttttgtttc tcccggggat attgtccatg catctttatt tcttccttcc atatcataac
      361 ttgaaatcct ttttacccgt gcatttttta ttttgcataa attaaacaat aaatttctca
      421 tatcccctcc ttttataatc ctttaatact ttttcaatta tacaacattt ccttatttct
      481 aaaaaatatc catctaaaac ccttaatcgt caacaattct ccaatcccac cttaaaggtg
      541 ggataaggga acaatttatt aatgtgccta taaacctcaa tgcatcatcc atcctttcaa
      601 gcatttcttt gtggtcttta tccatcctct ccattctttc gtctatcttt tccagtatct
      661 cttgtactct tttgttccct tcttccatca tcttttttgc tctttcatct taggtactta
      721 ttagttcatc tgtccttctt ccgagcacta tgggtaaaac aataacaact actacattta
      781 aaaacccaag aaaaaactca aaaatatcat cacattttcc attttcttcc cttttctttt
      841 atcccattgg gcaagtgtcc gacattcctc aaaaataaac ttgataaaaa gcatattaaa
      901 tgaaaaggtg aaaagaaaaa tctcttataa aaatataatt tcaaatagat ttaattttga
      961 ggaaatacgt tttttgactt ttatactctc tttaaattaa aatataataa aatctgtaat
     1021 tagtaaaaaa gaaatgagag cgcttattat cggagcaatt ttgtgtttta ttataggaac
     1081 tgctgatatt tataatcttg ttaaaattca aggttcttat atgacacttg attttacaac
     1141 tggttttgca atatttttta tattttttat tacacttttc aattatctct ttaaaaaaat
     1201 ttttaaaaaa gaagggttta agacagaaga actcataatt atctacatta tgatgattgt
     1261 ttcctgttca attcctacaa tgggacttac tttatatctt gttcctttaa ttgcaggagt
     1321 taaatattac tcaaatcctc aaaatgaatg ggatacaatt attcttcctc atcttaaaag
     1381 aaatttaata gttcaagatg aaaaagcgat aacatggttt tttgaaggaa taccaaaagg
     1441 agagaaatta ccatgggggc catggattaa accgctttct atttggattt ttttaattct
     1501 ttttatttat cttgcaatga tttttataat gattatattg cataaacaat ggtcagaaaa
     1561 tgaaaaatta aactatccaa tgacaaaagc accaattgaa cttataaaca gcgaaaatac
     1621 aaatattttt aaaaatggac ttttttggtt tggattttta atacctttta tatttggact
     1681 tataaatgga ttacactttt atttcccgaa tattccacca cttacattgg taagaaatgt
     1741 cccaatattt agaaagacat taaatttaca tttcagaata agttttccca tgatcggttt
     1801 tacatatctt gtaaatttac cacttgcctt ttctttatgg tttttctgcc ttttaacaac
     1861 gattgaacag ggttttttta atattacagg gtttggaagt caagaatttt taccttataa
     1921 tgctgataga cccttacttg gatggcaatc tcttggttct ttgattgtta ttgttttata
     1981 tggattatgg atttcaagaa aaaatttaat aaatataata agaacaaaag aagaagatga
     2041 aattatctca ccaaaaattt cgtttttcgg tttaattttt tctcttttat ttatttattt
     2101 ctggcttttg tattcaggta tttcaccttt acctgctatt ttatttatat tttttgcctt
     2161 tttaatttat ataggaataa caagggtaat atgtgaagga ggacttgcag caacaagagc
     2221 acctgtgata tcacctgtta taataaattc ttttcttggt tctactcgcc ttggaccctc
     2281 caatcttatt ggtcttggat taacctttgt ttattcttca gatgttagaa catttgttat
     2341 ggcttcagtt gcaaatggat tgaaaatggc tgaaaatata aaaaagaaaa aaagacttat
     2401 cttctgggca ataataattt caatttttat taccctattt tcgtcaatat gggcaactat
     2461 aattctatgt tataaatatg gtggaataaa tgcaaatcaa tggttttttg tagcgggacc
     2521 tcagtatcct ttaaagtatg ttgcagataa aataaaaaat cccaaaggtc ctgactggca
     2581 gtatattgga tttactggga taggcgcttt ctttacagtt tttttattta taatgagatt
     2641 gagattttta aattttcctt ttcatccact cggttttgct ttttccacaa taatgatgac
     2701 aaatgcttta tggttttcta tttttatttc ctggcttata aaggttttaa ttttaagata
     2761 tgggggagca aaaatttatg aaaaatttaa agggttattt attggtctta taatagggca
     2821 atttgttatc agtggtttat ttattatagt tgatctaatt acagggaaaa ctggaaatac
     2881 ccttttctgg gcataaaatt tgactatttt tgaaaaatca atgaaaagat ttataaaggg
     2941 gtaaatatga aaatatggag cagaattgta ggaacaattt atattttgat ggggatattt
     3001 tttgtattct gtcttttatc ttttttttca aatgaaaatt tatgcgaaag ttttataaat
     3061 tttataaaaa ataacttata taaagttgaa atttttgcaa ctattgtatt gattattgga
     3121 attatctatg ttgttaactg gattgattat atttatagaa caaaggcaat ttcttttgat
     3181 aatcctagtg gaaaagtaag agtatctcta aaagcaataa aagactcaat aactacaaca
     3241 attcttaagg aaatagatgg gataaaaaca attaaggtta aaacatttgt aactccgaaa
     3301 gggcttgaaa caaaaataaa tttaaaactt ttttcaaatc ttaatatacc tgatatatgc
     3361 agtaatattc aggaattaaa aaaaattatc ttcaagacac aattggagtt gaaagggtat
     3421 caaatataga gatttatgtt tcaaatatga taagtgaaat agaaacacat atggaagttt
     3481 tgaattctct tttaaaattt aatctttttg aaaaactcat tgaaatctct gagcttattg
     3541 taaaaagttt aaaaaaaggg aataagattc ttttatgcgg aaatagtgga agtgctgctg
     3601 actgtcaaca ttttgcaggt gaaatggtga atagatataa aaagaaaaga aggccattac
     3661 cttttatttc tttaactact gatacatcta tcataacaag cataggaaat gattataaat
     3721 ttgaagagat attttcaaaa caggttatgg cacttggtaa agaaaacgat attttaatat
     3781 gcttttcaac atctggtgaa tcaaaaaatg tcatagaagc ggcaaaagta gcgaaaaaat
     3841 taaaatgaat attatctctt ttacaggtaa atctccaaat atccttgaaa aaatctctga
     3901 ttacaatata tctgttccaa ccttacaaac agaaagggtt caggaaatac atctaataat
     3961 atatcatctt ttttcctatt taattgagtt agaattttaa aaatgtctaa aataaaaaac
     4021 ttctcagaga ttgaaaagat ttctaaaaga ttaaaaaaag aaggcaaaat tattgttttt
     4081 actaatggat gttttgatat tattcatcca ggccatataa aactattaaa aaaagcaaaa
     4141 tcacttggag atgttcttat tgttggatta aataaagaca aatcaataaa aaaaattaaa
     4201 ggagaaaaaa gacctataat tgatgaaaag gagagggcag aaattttatc ttcatttgaa
     4261 atgattaatt atgttgtttt atttggagaa aaaacaccag aaagattaat aaaaaaaata
     4321 cttccacatt atattgtaaa aggtggggat taaaaagaag aagatgttgt tggaaaagga
     4381 atagttgaac aattctccaa tcccacttta aaagtgggat aagggaacaa ctttattcaa
     4441 atcatacaga caaatttcac ataaagaaga tattgcttca tcatcaagaa ctgcccttat
     4501 aatcccaaga tgcctatgtg ctcctccaat aaacacaagt ttcatttttt ctccttaata
     4561 aacatcatct tccattattt ttacatactt tttatgtaat gcccaaaatt gttaaaaata
     4621 atagaattgt caataaaggg tatatattct cttaagtata cacgttccat aaccaccttt
     4681 atcctgatat aaaaattccc agcaactaat tttttttcgt tctccttttt aagggagaac
     4741 aatttcaaaa tatttaaaat aattaattgt taaaagtcaa atttttttga caaattttta
     4801 attaaattta aaataatcct gaaaaacaaa gggggtaatg gaattgaaaa aatggtttcc
     4861 taaaagagcg atacatcttg acttccacac gatgcctggg gtttatgatg tcggtattga
     4921 ttttaacggt aaagaatttg taaaaacact gaaagaagcg aatgtggatt atataacaac
     4981 ttttgcaaaa tgtaatcttg gttttgcata ttatccaaca aaaataggaa taatccatcc
     5041 aggacttaaa aaaagagacc ttcttggaga aattattgaa gaatgtcata aagaagggat
     5101 aatggttagt gcttatttta atgtgggact tgaccatgaa aattcagtaa ggcatagaga
     5161 ttggtgtaag gtaaataaag aaggtcagat tgcagaaatg cagtatatgg gacatttttt
     5221 cagaaagatg tgtctgaata cagattttaa agattatata cttggtatga ttgaagaagt
     5281 cattgataat taccctgttg atggaatttt ccttgactgt ttcactcttt ctccttgcta
     5341 tggtggtgaa tgtgtaagag aaatgaaaaa attgaatatt gacccttctg atgaaataaa
     5401 ggcaagggat ttttgctgga tgattacaga aaagtttatg gaggaagtta aaaaaatttt
     5461 aaaaaggaaa aataaagata tttttctttt tttcaatgga cttccttaca ggaaacagcc
     5521 aacgcattta gaacttgaag tccttccaac aggtggatgg ggatatgatt ttcttccatg
     5581 gattataaga tatgcaagaa cacttaaaaa accatttttc acaatgacag gaagatttca
     5641 taaaggatgg ggtgattttg gtggattgag aacattccat tctttaatgt ttgaccttta
     5701 ttattcagtt tcaaatgggg gaacctgttc aattggagac catatgcatc caagagggaa
     5761 acttgaaaaa gaagtttatg atttaatagg aaaatgttat tcagaaataa aaaaaattga
     5821 agaatttaca gaaaatgcag aaaatataac agagatggta attattgaac caaatctttc
     5881 aatgttttat ggttaccatt ttgattattc aagtttggca ggagcaacaa gaatgctttc
     5941 tgaacttaaa tatcagtttg atgtgagtga tgggacggat gatatatcaa aatataaaat
     6001 tgtaattctt cctgataata ttaaagttga taaaaacctt agagaaaaat tgaaaaaaca
     6061 tttgaaaaaa ggtggtattc taatcagttc tgacctttca tgttttgatt tagaaaagaa
     6121 aaaatttata tttgatgatt ataaaattga atttgaagga gaggaagaaa acaatccaac
     6181 ttattttgtt ggtgaaaaag aattttcaga tggaattcca gatatgcctt taacaattta
     6241 tgaccaggga atttcaataa aggcaaaaaa gggaagtaaa attattgctt atatttacaa
     6301 accgtatttc aattataaat catgggattg ggaacatgaa aatttatata ctccacctga
     6361 aaaaaataca ggaagaccag caattgtaca gtttggaaat ataatccatt tcagttttcc
     6421 agtttttaaa aattatcaca atcacgccta tatctattac aaaaaattac tgtataactg
     6481 ccttgaaaaa atttatccag aaccattgat taaacataaa aatattccat catttgtaca
     6541 gataactttg gcttctcaga aaaataaaaa tattatccat atacttgcat atatccctga
     6601 gttaagggga aaaagtatgc agataattga agaaccattg attttaaagg atatagaaat
     6661 aggagttaaa aatatagata aaaagaagat taaaagaatt taccttgtac cctcaaatga
     6721 agaaattgaa tttaaagaag aaggtaatta tatatggttc aaaattgaat caatggctgg
     6781 ttatcaaatg gttgtaattg aaaaaatgga ttaataaaat gaaagcagca ataactgaag
     6841 gaaaaggaga tgtaaaaata aaagaagttt caatacctga aataaatgat tatcaatgtc
     6901 tatgtaaaac acttgcctgt gctacctgta ccggaacaga ccttaaaata attgatggaa
     6961 agatgttctg gtgtaaaaac tatcctgtca ttcttggtca tgagagtgtt ggagttgtca
     7021 tcaaaaaagg caaaaaggtc agatatatta aagaaggaga gattttttta agaacgacct
     7081 gtgtttaccc tggagaaaaa attgatttat actggtcagc gattggagga ttttcagaat
     7141 acgggaaaat aacggatata aaggcgatga aggaagataa taaagaaaaa gaaataaatc
     7201 attatacagt ttatcaacag aaaatacccg aagaattaaa tatttctcct tttgatgcaa
     7261 caatttttat aacacttaaa gaaacagcaa gttttttatt aaatattggc tgtaaatttg
     7321 aaaattcatt gttaattctt ggttcaggac ctgttggaat gagtttggtt tatttttcaa
     7381 aattgattgg atgctttcca gttatagttg ctggaagaaa ttcaaaaaca cttgaacata
     7441 taaaaaaaat aggagcagat tttataataa atacagaaaa tgaaaaaatt gaaaaagtaa
     7501 aagagataac aaatggaaaa ggagttgact ttattattga taccacagga aatacagaat
     7561 ttgttttaag tgtaataaat cttctttcag aaaacggtaa aattgctcca tatgcaagtt
     7621 acagtgaacc atctccattt aaaaagtatg agaaaacagg aaaatttatt ctaaaaggac
     7681 cttctgaacc aataacacat aactatattt taaaacttac agaaatgaat attttgaaac
     7741 catctctttt ttattcacat attttaccat ttgaagaaat agaatatgga tttcaactaa
     7801 taagaaaaaa agaagcattt aaaattgttt ttaaaatgga ggtgtagaat gaaaattaag
     7861 gcattaatat atcaagaagc aaacaaattt gaaatatctg aattaacact tgatgaaatg
     7921 aaagatgagg atataaaagt taaaacaata atttctgcaa tctctcctgg aacagaaaga
     7981 tgggttttaa aagggaaaca tataggaaca aaatttccat gtgttcctgg atatcacaga
     8041 attggtataa ttgagaaagt tgggaaaaat gtaaaaaatt ttaaagaagg agatattgtt
     8101 tatggaattg ataatagatg gaaagaaaat atttatagaa tgtggggagc ccatgtttct
     8161 cattctgtag ggaattggaa aaattactgg ttcatttctt caaagatacc tgataacttt
     8221 gaacttgaaa caattgtttt tactcaactt tctgctgttg caacaagagg gataaaagca
     8281 cttgaagtaa aaagtagaga aaaaattgcc ataataggtg ctggatttct tggattatgt
     8341 gcatgtcaac tctcaattta tagaaatgca gtacctattt taatagataa agaccctgaa
     8401 agagttgaat tcggaaaaaa gtttactcaa ttttcatttt taattgatga taaagagatt
     8461 gatgaaaaat taaaagagat tgcacctgaa ggttttgacc atttatatga tactgttgga
     8521 gacccaaata ctacagataa acttgttcaa attacaaaat ttagagggaa aattttactt
     8581 caggcacagt attttgataa agaaaaatgt gctattgacc ttgaccagat aaaaataaga
     8641 gaaattacta taaaaacaac aataggaata gatacaattg atttttatga aactcttgac
     8701 tatataaaaa aaaggatttt aaaaatttct gaaatgataa cacacagatt tacaagtgaa
     8761 gaattgacaa aaggatttga aattttaagg gatgggaaac catttaatct tggaatactt
     8821 ttctggtgga aataaacaaa agggaaaact gaatgaaagg taatataaat ctaaaaatgg
     8881 ttgttaaaaa agaaggtgta gaacttttgt caagcaagta tatggaaata attgaaatat
     8941 aaaaacaggg aaaaaaatga gatatgaaaa tatgtttcca gaacaaataa gagaagcaat
     9001 taagaaaaat atacccatag ttttgcctgt tggagttata gaatatcaca gtgaacatct
     9061 atgtatagga gttgatactc tgcttgtttt aaaagcactt gaagagatag aaaaagaaat
     9121 ggaaattata attttaccac ctttttacta tggatgtgca agttatgcag ttgaacctcc
     9181 tgaaggaaat gggacaattc atataaagcc cgaggttata tacaaatttg caaaaaatct
     9241 atttgaaaat cttttaaggg ttggctttag aaatatttat gtcattattc atcatcaaag
     9301 tgaaaatttt attgcaggaa tgccaacaga ccttgctttt aaacttgctg caagagaaag
     9361 tatatttgaa tttcttgaaa aaagatatgg tgaaggatgg tggggaa
//
LOCUS       DAOSAT010000071         9405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_4793_length_9405_cov_4.864813, whole genome shotgun sequence.
ACCESSION   DAOSAT010000071 DAOSAT010000000
VERSION     DAOSAT010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4793_length_9405_cov_4.864813"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="PLF90_06860"
     CDS             complement(<1..1095)
                     /locus_tag="PLF90_06860"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOK57032.1"
                     /translation="MEKVLIFDTTLRDGEQSPGASLTSDEKLKIALQLEKLGVDIIEG
                     GFPISSPDDAKAVRIIGENVKKSIVCALARCRDEDIDIALKSLEKARKPRLHLFLATS
                     EIHRKYKLKKAKEEIIRIAKEKVKYAKKFIDDIEFSPEDASRTEPDFLLDVCKAVIEE
                     GAKTLNIPDTVGYAIPEEFGKLIKFLKENLPEDIIISVHCHNDLGLAVSNSLSAILNG
                     ARQVECTINGIGERAGNASLEEIVMNLVVRKNYYKVETNIKTQEIYKTSRLVSTLTGI
                     PVQPNKAIVGKNAFRHEAGIHQDGILKHRLTYEIMDPKMIGIPESELVLGKHSGRHAL
                     RERLKTLGFEFSDEKFEVIFEKFKKLAEKKK"
     gene            1179..1781
                     /locus_tag="PLF90_06865"
     CDS             1179..1781
                     /locus_tag="PLF90_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57033.1"
                     /translation="MRKVIFLICLCVLISGCSRNKINLEEVLSSEQIEEAINYGKENV
                     SLTFTEFTEKWTVGYEYEYGKGRATLITPFLRVALLSKNATEKHQKVEMNIINMALKD
                     ISDKLYFRVSLFGNYATFGRTVKFYLEYDGKKIQPLSYYMSPYSQFTRDYYHISEGEV
                     KFSNKEIPKDAKIKLVAVFKPYENEKEKSCSFEFDLSKYK"
     gene            1865..2869
                     /locus_tag="PLF90_06870"
     CDS             1865..2869
                     /locus_tag="PLF90_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK57034.1"
                     /translation="MKERPSLKDIARLAGVSKVVVYTVLKGRENKGIFVSKKTKEKIL
                     KIAEEIGYVPPKSAKELFTGRSDTIGIIFHKLTSFFSSLVELLQIEAQKDGLEITPYI
                     TNGDWKLEKHYLDICRDGRVDGIITVAHTDKSVELYKKYTKKPYNLKILSYGPYIEGV
                     PTIHFNEEKAGRLAAEHLLEIGCRKIAIFGGEKGSGRYNGFVNYLKERGNEPIIFVKD
                     KFVGYYEDGILLAEEFFNQSQIPDGVFAYNDLLGVILIKEAKKKGIKVPEDIAIIGCD
                     NSEVCLYSEVELTSIDTNIKEVVKKAIKLMKKMINGEEIPKKTIVDVKLIKRKSTERR
                     "
     gene            2874..3647
                     /locus_tag="PLF90_06875"
     CDS             2874..3647
                     /locus_tag="PLF90_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOK57035.1"
                     /translation="MKRENKINKKNRKEVNKMYKFLSTISHSRKRKGFTLIELLVVVA
                     IISILAAMLLPTLNNVRERARQITCMNNMKQIGMGYLMYTQDHDEYFPPLSDGKPEQS
                     FWYKGVTWLGYFPKNEYYKLTFQATAGRNPKMPCLWVCPTDLKVNRYKYGTSKDNLYW
                     IGSQYGSYGCNMSIMGRCPSPYTQSIKISKIKKPSQTLLIGESMVSPNRSATCLHFGG
                     LNPTRFKHNNFTFMNAFFVDGHAEAISVKNYGWIIITVP"
     gene            3668..7066
                     /locus_tag="PLF90_06880"
     CDS             3668..7066
                     /locus_tag="PLF90_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57036.1"
                     /translation="MMRIKEIIFCSLILSVLVQGGNTYIVPLMKKIPKIDGYINEDEW
                     EESIRFDGFIDSGNKLDLRKCIGFIGATDEKIYISIISEMPQNFNLISEVKQNSEEII
                     YDDNIEVWIDPYPENEIKTIYKMICNSIGYVYYSIFQKVKDESKVLEWAGGCRIKNGI
                     HNKMWHCEIEIPIKNIEKNRKITDGNWKINIVRNFKQPWTFTSIGSEFNNFISKNDNV
                     FKFSNDKGIIVKEKHLKDPFLGNIYYVCELYNPLKKEIKANVNISIKRFGNVEGFSKK
                     EEIILLPGEKKEVIVDKEDSKEYRFVLETSIQNIENNEVFFSRGPIYWKNFNILNWKE
                     RDEKWINYKKEIPGADFIISYYPYLNKIKIFTSIANILEKDGKVEKVRYKIINIETQR
                     EEISFYLGIEEFKNGLCEKIFDLPHLMGRYKITMQIIGKNIPEGEVIKYFERKSFEWE
                     HNNLGKSRKVYPPFIPIKKEGNKLYTVLKEYAVNNIGLLSSIITEDQQGINKKEILAG
                     EIKYKGKIDGKEENIEKSQLIYKEISEDRIIMESNFKIGKLEINSISFLDYDGMLKVD
                     LTINPCDVRVDELTLEIPIKNDVATLMHAMSTDIRDPILTTYVPPGEGLIWDSNKLPP
                     TQFNRVFPSNFCTYIYVGNEHRGISWFCENDKGWSRDEKKPNLQIVREKDVLKIQINL
                     INKPIIITKPQTITFGILAAPVKPKLKGWRYRWVKENFGLVATDSVWFGYPCCGSVYP
                     LGKDTYIFEILGKANRREKITEEEMKIFKEKLKKYSESLGKEQGIDRFLGNSMEVKLN
                     IQRNIKNTIFYYNRATFPAAEEFQTFMDEWALKEYNNHYRPYGIWELGVDGVWEEDIV
                     PTESYIDFAVWWYKKAIELANASVIYWDNYFFIPSTNTMTTSAYKKEDGTIMSSTGLW
                     GLRELVKRTFIMMNELGKEPFTMVHMTSTQILPLYSFATIQLDLEWKYSKEFMQNKFP
                     REYLRLVSSGDLPGTWPMVIGDEQTDKKAYIGVSIIHDIIDRTSQWYKQYRKPFIDMA
                     EKGENLIVYRYWDERQQPIYTKNPDLPGIVYCIPGKESLYGVVSYLKEDTEDTVFIHP
                     EILKLNKYKVIDMETNQEIPVENNTFKLKIKKYDFKVFKVVEIQ"
     gene            7082..7831
                     /locus_tag="PLF90_06885"
     CDS             7082..7831
                     /locus_tag="PLF90_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57037.1"
                     /translation="MRKILLLINLFLFSNLFGDDFQFLPERFRNNIVYFNDFEKKDAK
                     GKINLWGIEEKINLSRIVSDNVFGKCYLTSLKLPPKTGEPDETIILKSDKILFYKGLT
                     FSFWWRFKNEKNFPFVLFSLGMFSGIITNENFGQINFNLRNIKPIIGKFSVNPYNREI
                     KNTLITISAGGLEIKMYQNSQLVLDLISPRPMNEKDIFNQITIGYQGKTEILIDEILI
                     MDRPINLLEVKDYYERKKCYYENEIIKGGRK"
     gene            7828..9384
                     /locus_tag="PLF90_06890"
     CDS             7828..9384
                     /locus_tag="PLF90_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57038.1"
                     /translation="MRKILFLFFMFSIFLSCNNEYKNIAQVSIFGKEPKIDGKIDKDE
                     WNGAISTIGFQKITGKRQLDPRMGRTYFGFSEKYLYFLVISELPPDGKLITNCENRDS
                     SVIFDDGIEIWIDPFRDERENKDLQRTLSLYQCIVNSLGVVYDVKFKGGIPDTGWNGR
                     WEVANSIDKENKLWICEIRIPFEDLEIKENFYNRSIGVLISRNYKRPWEQATWFPHRG
                     EFLSWFEYTRIYLKRESVNIQILDLGEKFFEGQPHFKVKILNLTNNEKQIKAKIEIFS
                     SNLPSLSDEKILNILPKTSEIYEFKVPENRFHADADHYFDLKVSSMDNNEIYMMYRCL
                     WTKAPEEKWAVRVGPNPANAVRVAFLPTYGILKIRIDPSYLGEEWKDINSGEILVTNK
                     KGEIIYSSKLNWEKNPYEQKIKLGEIEEGEYTVNLKLNGYKEEFIRKFERKIFEWEGN
                     KFGITDKVYKPFLPIKVNGKEVEVVLRKYIQDNIGLWSSIKAKGVEEKSDYKELLASP
                     MVFILDGEKL"
BASE COUNT         3732 a         1020 c         1625 g         3028 t
ORIGIN      
        1 ttttttcttt tcagcaagtt ttttaaattt ctcaaatatt acttcaaatt tttcatcact
       61 aaattcaaat cccaatgttt tcaacctttc tcttaaagca tgtcttccag aatgttttcc
      121 aagaacaagt tcgctttcag gtatccctat catcttcggg tccattattt cgtaggtcaa
      181 tctgtgtttc aatattccat cctgatggat tcctgcctca tgtctgaaag catttttacc
      241 aacaattgcc ttgtttggtt gaactggaat accagttaaa gttgagacga gacgacttgt
      301 tttgtaaatt tcctgtgtct ttatatttgt ttcaacttta taataatttt ttctgacaac
      361 aagattcata acaatttctt ctaaagatgc atttcctgct ctttcaccaa ttccatttat
      421 tgtacattca acctgtcttg ctccatttaa aattgctgac agagaattac ttacagcaag
      481 tcctaaatca ttatgacagt gaacacttat aataatatct tcgggtaaat tttcttttaa
      541 aaattttata agtttcccga attcttcagg tattgcataa ccaactgtgt ctggaatatt
      601 taatgttttt gcaccttctt ctataactgc tttacagaca tcaagaagga aatctggttc
      661 tgttcttgaa gcatcttctg gagaaaattc aatatcatca ataaattttt ttgcatattt
      721 taccttttct tttgctattc ttattatctc ctcttttgct ttttttaatt tatattttct
      781 gtgtatttca ctcgttgcaa gaaaaagatg taatcttggt tttcttgcct tttcaagtga
      841 ttttaaagca atatcaatat cttcatctct acaccttgca agagcacata caatagactt
      901 tttaacattt tctccaataa ttcttactgc ttttgcatca tcaggacttg aaatagggaa
      961 tcctccttca ataatatcaa caccgagttt ttcaagttgt aaagcaattt taagtttttc
     1021 atcgcttgtt aaacttgctc ccggtgattg ctcaccatcc cttaaagttg tgtcaaaaat
     1081 caataccttt tccatttttc tcctttttta ttatattatc ataaaagttg accttaataa
     1141 agaagagcgt taaaataaat tactaaaagg gtaaaaagat gagaaaagtt atcttcttaa
     1201 tttgcctttg tgttttaata tctggatgta gtagaaataa aataaatctg gaagaagttt
     1261 tgagttctga acaaatagaa gaagcaataa attatggtaa agaaaatgtc tctttaactt
     1321 ttacagaatt tacagaaaaa tggacagtag ggtatgaata tgagtacggc aaaggcagag
     1381 ctacattgat tactcccttt ttaagagttg ccctgctttc aaaaaatgct acagaaaaac
     1441 atcaaaaagt tgaaatgaat attataaata tggctttaaa ggatatttct gataaacttt
     1501 atttcagagt ttctctgttt ggtaattatg caacatttgg aagaactgta aaattttatt
     1561 tagaatatga tggtaaaaaa atccagccat tatcatatta tatgagtcct tattctcaat
     1621 ttacaaggga ttattatcat atttcagagg gtgaagttaa gttttcaaat aaagaaatac
     1681 ctaaagatgc aaaaattaaa cttgttgcag ttttcaaacc ttatgagaat gagaaggaaa
     1741 aaagttgttc ttttgaattt gatttaagta aatataaata agccatttca aaaagttcaa
     1801 attcctcttg acaaaatttt aattatatag tataatatat cataggataa taaaggataa
     1861 taaaatgaag gaaaggccaa gtttaaaaga tattgcaaga ttagcaggtg tgtcaaaagt
     1921 agttgtatat actgttttaa aaggaaggga aaataaagga atatttgtaa gtaaaaagac
     1981 aaaagaaaaa atattaaaaa ttgcagaaga aataggatat gtcccaccaa aaagtgcaaa
     2041 agaattattt acaggaaggt ctgataccat aggaataata tttcacaaat taacttcatt
     2101 tttttcttct cttgttgaat tattacaaat agaggcacag aaagatggtt tggaaataac
     2161 tccttatata acaaatggtg attggaaact tgaaaaacat tatcttgata tatgtagaga
     2221 tggaagggtt gatggaataa taacagttgc tcatacagac aaaagtgttg agttatacaa
     2281 aaaatataca aaaaagccat acaatttaaa gatactttct tatggacctt atattgaagg
     2341 agttccaaca atacatttta atgaagagaa agcaggaaga ttagcagcag aacatttact
     2401 tgagattgga tgtagaaaga ttgctatttt tggtggagaa aagggttcag gaagatataa
     2461 tggatttgtg aattatttaa aagaaagagg gaatgagccg attatttttg taaaagataa
     2521 atttgttggt tattatgaag atggaatttt gcttgcagaa gaatttttta atcaaagtca
     2581 aattcctgat ggtgtattcg cttataatga tttacttggt gtaatcctta taaaagaggc
     2641 aaaaaagaaa ggaataaaag ttccagaaga tattgcaata attggatgtg ataattcaga
     2701 ggtttgttta tatagtgagg tagaattaac aagcatagat acaaatataa aagaagttgt
     2761 gaaaaaagca attaaactaa tgaagaaaat gataaatgga gaagagatac ctaaaaaaac
     2821 aatagtagat gtgaaattga taaaaagaaa aagtactgaa aggaggtgaa aatatgaaaa
     2881 gagaaaataa aattaataaa aagaatagga aggaggtgaa taaaatgtac aaatttttat
     2941 caacaatttc acatagtaga aaaagaaagg gcttcacatt gatagaattg cttgttgtgg
     3001 tagcaataat aagtattctt gcagcaatgc ttttacccac cttaaataat gtaagagaaa
     3061 gagcaagaca gataacttgt atgaataata tgaaacagat aggaatggga tatttaatgt
     3121 atactcaaga ccatgatgaa tattttcctc cactttcaga tgggaaacca gaacaaagtt
     3181 tttggtataa aggtgtaaca tggcttggat atttcccaaa aaacgaatat tataaactta
     3241 cttttcaggc aactgctgga agaaatccaa aaatgccttg tttgtgggtc tgtccaactg
     3301 accttaaagt aaatagatat aaatatggca cttccaaaga taatctttat tggataggaa
     3361 gtcagtatgg aagttatgga tgtaatatga gtattatggg aagatgtcct tccccgtata
     3421 cacaatctat aaaaatttca aaaattaaaa aaccttctca aacattatta attggagaat
     3481 ctatggtaag tccaaataga agtgctactt gtcttcactt tggaggatta aatcctacaa
     3541 gattcaaaca taataatttt acttttatga atgcgttttt tgttgatgga catgcagagg
     3601 caatttcagt taaaaattat ggttggataa taataactgt gccataaagt aaaaggagga
     3661 aaagtatatg atgagaataa aagaaataat tttttgtagt ttgatattat cagtattagt
     3721 acaaggaggg aacacttata tagttccttt aatgaaaaaa ataccaaaga tagatggata
     3781 tattaatgaa gacgagtggg aagagagtat cagatttgat gggtttatag acagtggaaa
     3841 taaattagac ctaagaaaat gtattggttt tataggtgca actgatgaaa aaatttatat
     3901 ttcaattatt tctgaaatgc ctcagaattt taatttaatt tctgaagtaa agcaaaatag
     3961 cgaagaaata atttatgatg ataatattga agtttggatt gacccttatc ctgaaaatga
     4021 gataaaaact atatataaaa tgatttgcaa ttcaattgga tatgtttatt attcaatttt
     4081 tcaaaaagtt aaagatgaga gtaaagtatt ggaatgggca ggagggtgta gaattaaaaa
     4141 cggtattcat aataaaatgt ggcattgtga aattgaaata ccaataaaaa acattgaaaa
     4201 aaatagaaaa ataacagatg gaaattggaa aataaatata gttagaaatt tcaaacagcc
     4261 gtggactttt acaagtattg gtagtgaatt taataatttt atttctaaaa atgataatgt
     4321 tttcaaattt tcaaatgata aaggaattat agttaaagaa aaacatctca aagacccttt
     4381 tttgggaaat atttattatg tttgtgaatt atataatcct ttaaaaaagg agataaaagc
     4441 gaatgtaaat atttcaataa aaagatttgg aaatgtagaa gggttttcaa aaaaagaaga
     4501 aattattctt ttacctggtg aaaaaaaaga agttatagta gataaagaag attcaaaaga
     4561 atatagattt gttcttgaaa catcaattca aaatattgaa aataatgaag ttttttttag
     4621 taggggacca atttattgga aaaattttaa tattttgaac tggaaggaaa gagatgaaaa
     4681 atggataaat tataaaaaag aaattccagg agcagatttt attattagtt attatcctta
     4741 tctaaataaa ataaaaatct ttacatctat tgcaaatata cttgaaaaag atggaaaagt
     4801 agaaaaagtt agatacaaaa taataaatat tgaaacacaa agagaggaaa taagttttta
     4861 tttaggtata gaagaattta aaaatggttt atgtgagaaa atttttgatt taccacatct
     4921 tatgggaaga tataaaatta ctatgcaaat aattggaaaa aatattccag aaggagaagt
     4981 gataaaatat tttgaaagga aaagttttga gtgggaacat aataatttag gaaaatcaag
     5041 aaaggtttac ccacctttta ttccaataaa aaaagaaggc aataagttat acacagtttt
     5101 aaaagaatat gcagtaaata atattggtct tttatcttca ataataacag aagatcaaca
     5161 gggtattaat aaaaaagaaa tacttgctgg agagataaaa tataaaggaa aaatagatgg
     5221 aaaagaagaa aatattgaaa aaagccaact tatatacaaa gaaatttcag aagatagaat
     5281 aataatggaa agtaacttca aaattggcaa attagagata aactctattt cttttcttga
     5341 ttatgatgga atgttgaaag tggatctaac tataaatcca tgtgatgtaa gagtagatga
     5401 attaactctt gaaataccaa taaaaaatga tgttgcaact ttaatgcatg caatgagtac
     5461 agatataaga gacccaattt tgactactta tgttcctcct ggtgagggat tgatatggga
     5521 ttctaataaa ttacctccta cacaatttaa tcgtgtcttc ccttctaatt tttgtactta
     5581 tatatatgtg ggtaatgaac atagaggaat tagttggttt tgtgaaaatg ataaaggttg
     5641 gtcaagggat gaaaagaaac ccaatttaca gattgtaaga gaaaaagatg tattaaaaat
     5701 ccagataaat cttattaaca aaccaataat tataacaaaa ccacagacaa taacatttgg
     5761 aatacttgct gcgcctgtaa aaccgaaatt aaaaggttgg agatatagat gggtgaaaga
     5821 aaattttggt ttagtggcaa ctgattctgt ttggttcggt tatccttgtt gtggaagtgt
     5881 ttatccatta ggaaaagata catatatatt tgaaatatta ggaaaggcga atagaagaga
     5941 aaaaattaca gaggaagaaa tgaaaatttt taaagaaaaa ttgaagaaat atagtgaatc
     6001 acttggtaaa gaacagggta ttgatagatt tttagggaat agtatggaag taaaactaaa
     6061 tattcagcga aatataaaaa atacaatttt ttattataac agagcaacct ttccagctgc
     6121 tgaagaattc cagacattta tggatgagtg ggctttgaag gaatataata atcattatag
     6181 accatatgga atttgggaat tgggtgttga tggtgtctgg gaagaagata tagttccaac
     6241 tgaatcatat attgattttg ctgtctggtg gtataaaaaa gcgatagaat tagcaaatgc
     6301 aagtgttata tactgggata attatttttt cattccatct acaaatacta tgacaacatc
     6361 agcatataaa aaagaggatg gaacaattat gtcatcaaca ggactttggg gattgagaga
     6421 acttgttaaa agaacattta taatgatgaa tgaacttggt aaagagccat ttacaatggt
     6481 tcacatgaca agtactcaaa tattgccttt atattcattt gctacaattc aacttgacct
     6541 tgaatggaaa tatagtaaag aatttatgca gaataaattt ccaagagaat atttaagatt
     6601 ggtttcatca ggagaccttc caggaacatg gcctatggtg ataggagatg aacaaactga
     6661 caaaaaagca tatataggtg tatcaataat tcatgacatc attgatagga cttctcaatg
     6721 gtacaaacaa tataggaaac cgtttattga tatggcagaa aaaggagaaa atttaattgt
     6781 ttatagatac tgggatgaaa gacagcaacc catatataca aaaaatccag atttacccgg
     6841 aatagtatat tgtattccag gaaaagaatc tttatatggt gtagtttcat atttgaaaga
     6901 agatacagaa gatactgttt ttatccatcc tgagatttta aaattaaaca aatacaaagt
     6961 aatagatatg gaaacaaatc aagaaatacc tgttgaaaat aatacattta agttaaaaat
     7021 caaaaaatat gattttaaag tttttaaggt tgttgaaata caataaaagg aaggagtaaa
     7081 aatgagaaaa attttattat tgataaattt gtttttattt tcaaatttgt ttggtgatga
     7141 ttttcaattc ttaccagaga gatttagaaa taatattgtc tattttaatg attttgaaaa
     7201 aaaagatgcg aaaggaaaga ttaacttatg gggaatagaa gaaaaaataa atttatcaag
     7261 gatagtatca gacaatgttt ttggaaaatg ttatttgact tctttaaaac tacctcctaa
     7321 aaccggggaa ccagatgaaa ctattatttt aaaatcagat aaaattttat tttataaagg
     7381 tttaactttt tctttttggt ggagatttaa aaatgaaaaa aatttcccgt ttgttctatt
     7441 ttcattaggg atgttctcag ggataattac aaatgaaaat tttggtcaaa ttaattttaa
     7501 tttgagaaac ataaaaccaa ttataggtaa atttagtgtt aatccctata atagagaaat
     7561 aaaaaatact cttataacca tttcagccgg tggtttagag ataaagatgt atcagaatag
     7621 tcaacttgta ttagatttaa tttcaccaag accaatgaat gaaaaagaca tatttaatca
     7681 aataaccatt gggtatcaag gaaaaacaga aattttgata gatgagattt taataatgga
     7741 tagaccaatt aaccttttag aagtaaaaga ttactatgaa agaaaaaaat gttattatga
     7801 aaatgagata ataaaaggag gaagaaagtg agaaaaattt tatttctttt tttcatgttt
     7861 tcaatttttt taagttgtaa taatgagtat aaaaatatag cacaagtatc tatttttggg
     7921 aaagaaccaa aaattgatgg taaaattgat aaagatgagt ggaatggtgc tatctctaca
     7981 ataggatttc agaagattac aggaaaaaga caacttgacc caagaatggg aagaacttac
     8041 tttggttttt cggaaaaata tttatatttt ttagttataa gtgaattacc acctgatggg
     8101 aaattaataa cgaattgcga aaatagggat agtagtgtaa tttttgatga tgggatagaa
     8161 atttggattg acccattcag agatgaaaga gaaaataaag atttacaaag aactttatct
     8221 ctttatcaat gtattgtaaa ttctttaggt gttgtttatg atgtaaaatt caagggggga
     8281 ataccagata ctggttggaa tggtagatgg gaagttgcca attctattga taaagaaaat
     8341 aaattatgga tttgtgaaat acgaatacca tttgaagatt tagaaattaa agagaatttt
     8401 tataataggt caataggtgt tttgatttca aggaattata aaaggccttg ggaacaggca
     8461 acatggtttc cacatagagg agaattttta agttggtttg aatatacaag aatttattta
     8521 aaaagagaat ctgtaaacat tcaaatactg gaccttggtg aaaaattttt tgaaggtcaa
     8581 ccacatttta aagtaaaaat tcttaactta acaaataatg aaaaacaaat aaaagcaaaa
     8641 attgaaattt tttcttcaaa tttgccatca ttatctgatg aaaaaatttt aaatatttta
     8701 ccaaaaacat cagaaatata tgagtttaaa gtccctgaga acagatttca tgcagatgct
     8761 gaccattatt ttgatttaaa agtttcatca atggataata atgaaattta tatgatgtat
     8821 agatgtttat ggacaaaagc accagaggaa aaatgggcag taagggttgg cccaaatcca
     8881 gcaaatgcag taagagtagc ttttttacca acttacggaa ttttaaagat aagaattgat
     8941 ccaagttatt taggtgaaga gtggaaagat attaattctg gagaaatatt agttacaaat
     9001 aaaaaaggag aaataatata ttcctcaaaa ttgaactggg aaaaaaatcc ttatgaacaa
     9061 aagattaagt taggtgaaat agaagaagga gagtatacag tcaatttaaa gttaaatggg
     9121 tataaagagg agtttataag aaaatttgaa agaaaaatat ttgaatggga agggaataaa
     9181 tttggaatca ctgataaggt atataaacca tttttaccaa ttaaagttaa tggaaaagaa
     9241 gttgaagtag ttttgcgaaa atatatccaa gataacattg gtttatggag ttcaataaaa
     9301 gcaaaaggag ttgaagagaa aagtgattat aaagaattgc ttgcttctcc tatggtattt
     9361 attttagatg gagagaaatt ataaggaaag ggtaaattca caaaa
//
LOCUS       DAOSAT010000072         9050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_5033_length_9050_cov_5.615231, whole genome shotgun sequence.
ACCESSION   DAOSAT010000072 DAOSAT010000000
VERSION     DAOSAT010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5033_length_9050_cov_5.615231"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1495)
                     /locus_tag="PLF90_06895"
     CDS             complement(176..1495)
                     /locus_tag="PLF90_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOK57039.1"
                     /translation="MKNLFLLLFLVLSIFYSNLFSESNFPLKDGDTWVFLGDSITAQK
                     LHTNYFEGYCFTRFPKWNIHFRNSGVGGDTIPRALARFDWDVAAWKPTIVSVELGMND
                     SGAGPDSVSIYINGMETLIKKIKEIGAMPVIFSPSPVNDGSTQNNLIGRNITLDKYTV
                     ALEEFTKNNSIIFADQFHFLVNLWGKNKTIEEIYRFADLTRNILTKPDLPAPELLKKW
                     LEEMGKSEILKKGVNIGGDPVHPGPVGQLMMCAILLEKLNAPKLISKAVIDISNKKIE
                     QVQCKITNLKIEEEKISFDRIDDCLPMPIPPEAKDALIIYPQIADLSQWILKIENLKK
                     GEYEIFIDGVSVATVSSEELKNGWNLSTVFKGPVYQQCLEVLSAISLKENLVSEWRKL
                     SSLQAKSPTEENKTKLQNLTEQILQADQKIKETAQPKIHNFTISLKK"
     gene            complement(1564..2754)
                     /locus_tag="PLF90_06900"
     CDS             complement(1564..2754)
                     /locus_tag="PLF90_06900"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOK57040.1"
                     /translation="MVEIKPFKGYIYNLDKIGEISKVISPPWDLINEELEKKLLSLSE
                     FNIVKLISKENNPDDVYETLNNWIENKILIQDDRENFYFLKENFEYDGKNYERKGIFG
                     ILKIEDFEKRNIIPHERIFQKYSDNRYKILEKCKSNFCPVFMLYPDKCFQIENIIDEE
                     KNYFTGYINNEKFSFGRIESREKIMKIEEIFKEKVVFIADGHHRYNASLMFYKNNPEE
                     RNKYLLVYLANIESDGVIILPTHRYIPSEVNLNFDKNFVDILKVNDKEEMEKLLGTSK
                     ERKIGMFYNGFFYILKIKNYEKQFKEEGPYLKLDTFLVDNFLIKNFVDIKENVEFLYH
                     SSKEYLLNEYKKRKNGVIFFLNPVKKDIFIEMCLNGKLMPQKSTYFFPKVPSGLVIYV
                     FPDT"
     gene            complement(2747..3454)
                     /locus_tag="PLF90_06905"
     CDS             complement(2747..3454)
                     /locus_tag="PLF90_06905"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOK57041.1"
                     /translation="MAFKFLRKRKNVRIILWIITVLIIPGFFLWGVGIEGSRKTQYAA
                     IVNREPITLREFYDQLWKIEEKYREIFGESYNQIRNKLNLEKNVLESMIREKILLQEA
                     RKRRIRVYKNEIINVMKSDPVFKNEKGEFDEEKYKQIISNYPDEELAKIEDQIKKEIL
                     IQKLKDQVVSETNINVDEKEIDDYLKNAGTEKIDRENIRKMILWQKREKYFEDWYKKT
                     REKSKVMIYLTLENKNG"
     gene            complement(3444..4118)
                     /gene="rsmI"
                     /locus_tag="PLF90_06910"
     CDS             complement(3444..4118)
                     /gene="rsmI"
                     /locus_tag="PLF90_06910"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOK57042.1"
                     /translation="MLYIIGTPIGNIKDITLRGLDVLKSVDLILCEDTRETSKLLNYY
                     NIKKPLLSYYKEIEKKRVKKVIELLKEGKEIALVCDRGTPGISDPAYLLVNEAYRNNI
                     KVVPIPGPSALTAAMSISGFPLKKVYFLGFLPKKEIEKRKIFERFKEGEEAVIFFESV
                     HRIDKTINLLAEIMPEKEICICRELTKMFEEILRGKIKDINEVFKKHKKKGEFVLIIN
                     GGENGF"
     gene            4203..4967
                     /locus_tag="PLF90_06915"
     CDS             4203..4967
                     /locus_tag="PLF90_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK57043.1"
                     /translation="MEKIGVIAYNFSFQRLEDIFNWCSENSVEYIEISRKNLEEALML
                     TKKYNVKVSQVSAGNDFLQKTREEFLEQIKLMGELCKRIKDIDCNIIRMDGGWPKEGV
                     DTSDYKKLIIEGVERSVEFAEKENVFLALDNHGTTTNDYEFQLEIFEKIKSKHLGANL
                     DTMNYRWYGYPVEKLIEIYKAIAPYTLHTHIKDGTGTRGDYVGKVLGEGEIPVIEAIK
                     ILKENNYKGVWCVEYEGKDKEIGYKKCVEFLRKLNL"
     gene            4972..6516
                     /locus_tag="PLF90_06920"
     CDS             4972..6516
                     /locus_tag="PLF90_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOK57044.1"
                     /translation="MKRERWGSKLGVILAVAGSAVGLGNFLRFPVQAVKNGGGAFIIP
                     YFISLFLLGIPLMWIEWTIGRFGGGFGHGTAPGIFHTLWKKNRFIKYFGVIGIFGPIA
                     IFIYYIYIESWLLAYVFFPFRSDFSSITTQNEMVAFLQGYQGVVKNQYFSSILPAYFF
                     FIITFFLNLLIVYYGIKKGIENVCKIAMPSLFIFAIVLVIRVLTLKNPANPDWSPLNG
                     LGFLWNPNFSSLKDAKVWLSAAGQIFFTLSVGIGVILTYASYLSKDDDVLLSGLTSAS
                     ANEFAEIILGGSIVIPAAFVFFGPVGIKEVANQGTFNLGFVTMPLILKSLPFGKIFEI
                     LWFFLLFLAGLTSSISLAQPAIAFLEDEFNLSKKRSVLVFGILCFLLSNLSVLFLAKG
                     VVDELDFWGGTFSLVVFATVETILFAWVFGMENAWEEMHSGSDITLPKFYKFIIKYIT
                     PLFLICILISWTVQYAIPTLLLKNIPKENIPFVFMTRIILLLLFIVLCILVKISWSKK
                     GEKIET"
     gene            complement(6598..7494)
                     /locus_tag="PLF90_06925"
     CDS             complement(6598..7494)
                     /locus_tag="PLF90_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOK57045.1"
                     /translation="MENKGRTGLSAYSLNIFRECERCFYLQVKYNITRPRGPMPSIAT
                     GIDSLIKDYFEHCRKKEKLPPFLKGKVEGKLITDLKKTYYYDIENFKNYYLWGRLDEV
                     IQLPDGLYIPLDHKTRANLPKGEPHDAYKFQLGVYSLLLKKENKGKEVDFGYLVYYYP
                     EKKFTDYDADEIKHIINFNFEVKEVKLDINDIKKNVENAINCLELEKLPESGKDCEYC
                     KWIENTKKIYYIGSTIGEIKKEKVEEGIIVKVEEIKEENIKDIPEKSVEMETTEIEEK
                     KLISPQEIKKGKKKKELPESLF"
     gene            7576..>9050
                     /locus_tag="PLF90_06930"
     CDS             7576..>9050
                     /locus_tag="PLF90_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOK57046.1"
                     /translation="MISKISSFTIWGIDAYPIEIEVDISKGVPGISIVGLPDQAVKES
                     KDRIKPAIKNSGYDFPSGKITINLAPADLKKEGPFFDLPMAIGILTANGEIKQSRVDE
                     FYFVGELALNGEIRPVSGILPMVLKLKEMKIKKIILPFENSLEGSIIKEVDVYPVRNL
                     NECIEFLKGKKEIEPVKSDIEEIFNKRNKYDVDFKEVKGQRYVKRAVEVAVSGGHNIL
                     MIGSPGSGKTMIARRIPTILPSLTLEEALEITKIHSVSGILKEPIVFERPFRSPHHTI
                     SDIALIGGGAIPKPGEVSLAHNGVLFLDEMAEFHRDVLEGLRQPLEDGKVNISRAKGR
                     LEFPSRFLLVAATNPCPCGWYGDSQRECHCTLSQILKYRKKLSGPLLDRIDIHIEITS
                     LPANILFEEKEEEDSESIRKRVEKAREIQKERFKNEGIYFNAHMNSSQIKKYCILSDE
                     AKYLLKNAIENLKISARAYDKIRKVARTIADLDNSEKILPH"
BASE COUNT         2928 a         1342 c         1313 g         3467 t
ORIGIN      
        1 tatcaatatt ctttatccag tcatatccta tttcaagttc aaatttccct ttttcaattg
       61 tctctgcatc atccgttgtt aaaggtcttc ctgcataaag aaatgttgag aaaaagaaaa
      121 ttgaaatact gaaaactgcc gatacgcctc ttttcatttt tcgcatcctt ttgtgttatt
      181 ttttaagaga gattgtaaag ttgtgaattt ttggttgtgc agtttctttt atcttctggt
      241 ctgcctgaag tatctgttct gttaaattct gtagttttgt tttattttct tctgttggtg
      301 attttgcctg taaagaagac aattttctcc attctgaaac aagattttct ttgagagaaa
      361 ttgccgaaag aacctctaaa cactgctgat atactggacc tttaaaaaca gttgataaat
      421 tccatccatt tttaagttct tctgatgata cagttgcaac tgatactcca tctataaaaa
      481 tttcatattc accctttttc aaattttcta tctttaaaat ccattgactt aaatcagcga
      541 tttgcggata aattatcagg gcatcctttg cttccggtgg tattggcatt ggcaggcaat
      601 catcaatccg atcaaaagaa attttttctt cctcaatttt taaatttgta attttacact
      661 gaacttgctc tatttttttg tttgaaatat ctattacagc cttacttata agttttggtg
      721 catttaattt ttcaagtaaa attgcacaca tcataagttg tccaacaggt ccaggatgaa
      781 cagggtctcc tcctatattt actccttttt ttaatatttc gctttttccc atttcttcaa
      841 gccacttttt taaaagttca ggtgcaggta gatcaggttt tgtgagtata tttctggtaa
      901 ggtctgcaaa tctataaatt tcttcaattg ttttattctt tccccataaa tttacaagaa
      961 aatggaactg gtctgcaaaa attatactgt tatttttagt aaattcttca agtgcaactg
     1021 tatatttatc cagagttata tttcttccta ttaaattatt ctgtgtactt ccgtcattca
     1081 caggacttgg agaaaaaata acaggcattg ctcctatttc ttttattttt tttataagtg
     1141 tctccatccc atttatatag atagaaacac tatcaggacc tgctcctgaa tcattcattc
     1201 caagttcaac actaacaata gttggtttcc atgcagcaac atcccagtca aatcttgcaa
     1261 gagcacgagg tattgtatct ccaccaacac ccgaatttct gaagtgaata ttccatttcg
     1321 gaaaacgagt aaaacaatat ccttcaaaat aatttgtatg aagtttttga gcagtaattg
     1381 aatctccgag gaaaacccat gtatcaccat ctttcaatgg gaaattactt tcagaaaaaa
     1441 gatttgaata aaaaatactt aaaaccagaa ataacaacaa aaatagattt ttcataaatc
     1501 ctcctttttg attaatctta ctacttttaa aaaaaaattt caaaattttg aattttattt
     1561 tttttacgtg tctgggaaaa cataaattac aaggcctgaa ggaaccttag gaaagaaata
     1621 agttgatttc tgtggcataa gttttccatt taaacacatt tctataaata tgtctttttt
     1681 aacaggattt aaaaagaaaa taactccatt ttttcttttt ttatattcat ttaatagata
     1741 ttcctttgaa ctgtgataga gaaattcaac attctctttt atatcaacaa aatttttaat
     1801 caaaaaatta tcaactaaaa atgtatcaag ttttaaatat ggaccttctt ctttaaattg
     1861 tttttcataa ttttttattt ttaagatata aaaaaaacca ttataaaaca tccctatttt
     1921 tctttcttta ctcgtaccca gtaatttttc catttcttct ttatcgttta ctttaaggat
     1981 atcaacaaaa tttttatcaa agtttaaatt tacttctgaa ggaatatatc tgtgagtggg
     2041 taaaattata actccgtctg attcaatatt tgcaagataa acaagtaaat acttatttct
     2101 ttcttctgga ttatttttgt aaaacattaa agaggcgttg tatctgtggt gaccatctgc
     2161 aataaaaact accttttctt taaaaatttc ttctatcttc ataatttttt ccctgctttc
     2221 tattctacca aaactgaatt tctcattatt tatgtaacca gtaaaatagt ttttttcttc
     2281 atctattata ttctctattt gaaaacattt atccggatat aacataaaaa cagggcagaa
     2341 attactttta catttttcaa gaattttata tctgttatct gaatattttt gaaaaattct
     2401 ttcatggggt ataatatttc ttttttcaaa atcctctatc tttaaaatcc caaaaattcc
     2461 tttcctttca tagtttttcc catcatattc aaaattctct tttagaaaat aaaaattttc
     2521 tctatcgtcc tgaattaata ttttgttttc aatccaatta tttaaagttt catatacatc
     2581 atcaggatta ttttctttag aaatcaattt tacaatatta aattcagata atgataaaag
     2641 ttttttttca agttcttcat ttattaaatc ccatggagga gaaataactt tacttatttc
     2701 tccaattttg tcaagattat aaatatatcc tttgaaaggt ttaatttcaa ccatttttat
     2761 tttcaagtgt taagtaaatc ataacttttg atttttctct tgttttttta taccagtcct
     2821 caaaatattt ttcccttttc tgccataaaa tcatttttct tatattttct ctatcaattt
     2881 tttcagttcc tgcatttttt aaataatcat ctatttcctt ttcatccaca tttatatttg
     2941 tttccgatac aacctggtct ttgagtttct gaattaatat ttcttttttt atctggtctt
     3001 caatttttgc gagttcttca tcaggatagt tacttattat ctgtttatat ttctcttcat
     3061 caaattcacc tttttcattt ttaaaaaccg ggtcggattt cataacattt attatttcat
     3121 ttttataaac tcttattctc ctttttcttg cttcctgtaa taatatcttt tccctaatca
     3181 tactttcaag aacatttttt tcaagattta atttattcct gatttgatta taactttccc
     3241 caaaaatttc tctatatttt tcttcaattt tccataactg gtcataaaat tctcttaaag
     3301 ttatcggttc cctgttaaca attgctgcat actgtgtttt tctgcttcct tcaattccaa
     3361 ctccccataa gaaaaaaccg ggaataataa gaactgtaat aatccagagt ataattctga
     3421 cattttttct tttccttaaa aatttaaaag ccattttcac ctccatttat aattaaaaca
     3481 aattctcctt ttttcttatg ttttttaaat acttcattta tatctttaat ttttcctctt
     3541 aaaatttctt caaacatttt ggtcaattcc ctgcaaatac atatttcttt ttctggcata
     3601 atttcagcaa gtaaatttat agttttatca attctatgaa cagattcaaa aaaaataact
     3661 gcttcctctc cttctttaaa cctttcaaaa atctttcttt tctcaatctc tttttttggc
     3721 aaaaacccta aaaaataaac ttttttaaga ggaaaacctg aaatactcat tgcagcagtt
     3781 aaagcagatg gacccggaat cggaaccacc tttatgttgt ttctatatgc ctcatttaca
     3841 agtaagtaag cagggtctga gattccaggt gtccctctat cgcaaacaag tgcaatttct
     3901 ttaccttctt ttaacaattc tataaccttt tttactctct ttttttctat ttctttgtaa
     3961 taacttaaaa gaggtttctt tatattatag taattcaaaa gtttacttgt ttcccttgta
     4021 tcctcacata atatcaaatc aacacttttt aaaacatcaa gccctcttaa agtaatatcc
     4081 ttaatattac ctatgggagt tccaattata tatagcatta aaatattata cttcaaaaac
     4141 ttcaaatttt ccagaaaata tattaaaata attttaaagt taaaaacaaa aaaggagaaa
     4201 aaatggaaaa aataggtgtg attgcatata atttttcatt tcagagatta gaggatattt
     4261 ttaactggtg cagtgagaat tctgttgagt atattgaaat ttccagaaaa aatttagaag
     4321 aagcattgat gttgacaaaa aaatacaatg taaaggtttc tcaggtaagt gcagggaatg
     4381 attttttaca gaaaacaaga gaagaatttt tagaacagat taagttgatg ggtgagttat
     4441 gcaaaagaat taaagatata gattgtaata taataaggat ggatggtggc tggcccaagg
     4501 aaggagttga taccagtgat tataaaaaac tgataattga aggtgttgaa aggagtgtag
     4561 aatttgcaga aaaagaaaat gtttttcttg ctcttgataa tcatggaaca acaacaaatg
     4621 attatgaatt tcaacttgaa atatttgaaa agataaagtc aaaacatctt ggtgcaaatc
     4681 ttgatacaat gaattataga tggtacggtt atcctgtaga aaagttaatt gaaatatata
     4741 aagcgatagc accttatact ttacacactc atataaagga tgggacagga accagaggag
     4801 attatgttgg aaaggttctc ggcgaagggg aaattcctgt aattgaagca ataaaaatct
     4861 taaaggaaaa taattataag ggtgtttggt gtgttgaata tgaagggaag gataaagaaa
     4921 taggttataa aaagtgtgtt gaatttttaa ggaaattaaa tctataagaa tatgaaaaga
     4981 gaaagatggg gaagtaagtt aggagttata cttgctgttg cgggaagtgc agtcggactt
     5041 ggtaatttct taagattccc tgttcaggca gtaaaaaatg gcggtggtgc ttttataatt
     5101 ccctatttta tctctctttt tttgcttggt atacccttaa tgtggataga atggacaata
     5161 ggaagatttg gaggtggttt tggacatgga actgctcctg ggatatttca tacattatgg
     5221 aaaaaaaaca gatttattaa atattttgga gttataggaa tttttggacc aatagccatt
     5281 tttatttatt acatatatat tgaatcatgg cttctcgctt atgttttttt ccctttcaga
     5341 agtgattttt cttcaattac tacccagaat gagatggttg catttttaca gggatatcag
     5401 ggagttgtta aaaatcagta tttttcatca attctgcctg catatttttt ctttataata
     5461 accttctttt taaatctttt aatagtttac tatggtataa aaaaagggat agaaaatgta
     5521 tgtaaaattg caatgccatc gctttttatt tttgctatag ttttggttat cagagttctt
     5581 actctgaaaa acccagcaaa tcctgattgg tctcctttaa atggacttgg atttttatgg
     5641 aatcctaatt tttcttcttt aaaggatgca aaggtctggc tttcagcagc aggccagata
     5701 ttttttactt tgagtgtggg cattggagta attcttacat atgcaagtta tttaagtaaa
     5761 gatgatgatg ttttactttc aggtttaacc agtgcaagtg caaatgagtt tgctgaaatt
     5821 attcttgggg gaagtatagt tattcctgct gcctttgttt tttttggtcc tgttggtatt
     5881 aaagaagttg caaatcaggg aacttttaat ctgggttttg ttacaatgcc tttaatttta
     5941 aagtcacttc cttttggaaa aatttttgaa attttatggt tttttttact tttccttgct
     6001 ggacttacct cttcaatttc tcttgctcag ccagcaattg catttttaga agatgaattt
     6061 aatttgtcaa aaaaaagaag tgttttagtt tttggtattt tgtgtttttt gctttcaaat
     6121 ctttctgttt tgtttcttgc aaaaggagtg gttgatgaac ttgatttctg gggaggaact
     6181 ttttcattgg ttgtttttgc aacagttgag acaattcttt ttgcatgggt ttttggtatg
     6241 gagaatgcat gggaagaaat gcattcaggt tcagatataa cacttcctaa attctacaag
     6301 tttataataa aatacataac tcccttattt ttgatatgta ttttaatttc atggacagtt
     6361 cagtatgcta ttccaacatt gcttttgaaa aatataccaa aagaaaatat cccttttgtt
     6421 tttatgacaa gaattattct tcttttactt tttattgtgc tctgtatact tgttaaaatt
     6481 tcatggagta aaaaaggaga aaaaattgaa acttagcgga tggtttatgc taggtatttc
     6541 atggagtgtt ataacttttc tttttgttta ttctttttta aggtcaatta aaaaaagtta
     6601 aaaaagagat tctggaagtt ctttcttttt cttccctttt ttaatttctt gtggagaaat
     6661 taattttttt tcttctattt cagttgtttc catctctact gatttttcag gtatatcttt
     6721 aatattttct tctttaattt cttctacctt tacgataatc ccttcttcta ctttttcttt
     6781 ttttatttct ccaatcgtgc ttcctatata ataaattttt tttgtgtttt ctatccattt
     6841 acagtattcg caatcttttc cactttcagg taatttttct aattcaagac aatttatagc
     6901 attttcaaca ttttttttga tatcatttat atccagtttt acttctttaa cctcaaaatt
     6961 aaagtttatt atgtgtttga tttcatctgc atcatagtca gtaaattttt tttcaggata
     7021 gtaataaaca agatagccaa aatcaacttc ttttccttta ttttcttttt tcaaaagaag
     7081 tgagtaaaca ccgagttgaa acttatacgc atcgtgtggt tcccctttag gtaagtttgc
     7141 tcttgtttta tggtcaagtg gtatataaag tccatctggt agttgtatta cttcatcaag
     7201 acgtccccag agataataat ttttaaaatt ttcaatgtca tagtagtatg tttttttaag
     7261 atcagttatt aattttcctt caacctttcc tttaagaaaa ggtggtaatt tttctttttt
     7321 tctgcaatgt tcaaaatagt cctttattag agaatcaata cctgttgcaa ttgagggcat
     7381 tggtcctctc ggtcttgtga tattatattt tacctgtaaa taaaaacatc tctcacactc
     7441 tctgaaaata tttagagaat atgcagataa tcctgttctt cctttatttt ccatagttta
     7501 attttataat tatttgaaaa cttatgtcaa cttaatttat aatttatgaa aaaataagaa
     7561 gtttggagat ttaaaatgat ttcaaaaatt tcttctttca cgatatgggg aattgatgca
     7621 tatccgattg agattgaagt tgatatttca aaaggagttc ccggtatttc aattgttgga
     7681 cttcctgacc aggcagttaa agaaagtaag gatagaataa aaccagcaat taaaaattca
     7741 ggatatgatt ttccatcagg taaaataaca ataaatcttg ctccagcaga ccttaaaaaa
     7801 gaaggtcctt tttttgacct ccctatggcc attggaatac tcactgcaaa tggagaaata
     7861 aaacaatcaa gggttgatga attttatttt gttggagaac ttgctttgaa tggagaaata
     7921 agaccagttt caggaatttt acctatggtt ttaaagttaa aagaaatgaa aataaaaaaa
     7981 attattcttc cttttgagaa ctctcttgaa gggtcaatca taaaagaagt ggatgtttat
     8041 cctgtaagaa atttaaatga atgcattgaa tttttaaaag gaaaaaaaga aattgagcct
     8101 gttaaatcag atattgagga aatttttaat aaaaggaata aatatgatgt ggattttaaa
     8161 gaagtaaaag ggcagaggta tgttaaaaga gcggttgaag ttgctgtaag cggaggacat
     8221 aatattttaa tgattggttc acctggaagc ggaaaaacaa tgatagcaag aagaatacca
     8281 acaatcttac cctctcttac actggaagaa gcacttgaaa taacaaaaat ccattcagta
     8341 agcggtattt taaaagaacc cattgttttt gaaagaccat ttagaagtcc acatcataca
     8401 atttcagata ttgcattaat tggtggtgga gcaattccaa agcctggaga agtcagtttg
     8461 gcacataatg gtgttttatt tcttgacgag atggccgaat ttcatagaga tgttcttgaa
     8521 ggactcagac aacctcttga agatggaaaa gtaaatattt caagagcaaa aggccggctt
     8581 gaatttcctt caagattttt acttgtagca gcaaccaatc catgtccatg tggttggtat
     8641 ggagacagtc agagagaatg ccactgcaca ttatcacaaa ttttaaaata cagaaaaaaa
     8701 ttatcaggtc cattacttga tagaattgat atacatatag aaattacaag tttacccgca
     8761 aatattttgt ttgaagaaaa agaagaggaa gattcggaaa gtataagaaa aagagtggaa
     8821 aaggcaagag aaatacagaa agaaagattt aaaaatgagg gtatatattt taatgctcat
     8881 atgaattctt ctcagataaa aaaatattgt attttatcag atgaggcaaa atatttactg
     8941 aaaaatgcaa ttgaaaatct aaaaatatct gcccgtgcat atgataaaat aagaaaagtg
     9001 gcaagaacta tagcagacct tgataattca gaaaagattt tacctcatca
//
LOCUS       DAOSAT010000073         8991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_5075_length_8991_cov_4.511526, whole genome shotgun sequence.
ACCESSION   DAOSAT010000073 DAOSAT010000000
VERSION     DAOSAT010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8991
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5075_length_8991_cov_4.511526"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /gene="rsxC"
                     /locus_tag="PLF90_06935"
     CDS             <1..929
                     /gene="rsxC"
                     /locus_tag="PLF90_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOK57047.1"
                     /translation="REAGIVGLGGAAFPTHVKLTIPEGKKADYILINGCECEPFLTCD
                     YRVLKEYTEEVVEGIQIISKVTNVKKVFIGIERNKIDLLPVLNDAIKKLKIYIEIEIK
                     ILPEKYPQGSEKHLIKSILNKEVPSGGLPIDVGCIVFNVQTTLAIKRAVCDGIPLTER
                     VLTCTGLVDNPKNLKVKIGTPISEIIDYCKGDIKNGSKIVVGGPMMGIQIPNPDVPVI
                     KGTTGILLLPEEKLPEDIQPCIRCGKCIEVCPMFLMPSEIGRYMEFEKVEKCENLNVF
                     DCIECGCCAYICPAKRPLVDFIKCAKFKLRKK"
     gene            932..1864
                     /locus_tag="PLF90_06940"
     CDS             932..1864
                     /locus_tag="PLF90_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOK57048.1"
                     /translation="MEKVCKINCSPHIHSSETTGKIMFYVVISLIPALIGSIYFFGIY
                     VLEVIIISIISCIVSELLFKILTRKKIEVFDGSAIVTGILLAFVLPPRIPLWIAGIGG
                     FLAIFLVKELFGGIGFNIFNPALASRAILLASYPVEMTKFIQPFDYRIDAITSATPLF
                     IIKEKLNQQLPSLWQMFIGTRPGCIGETSTLLLLIGGFFLIYKRVISWHIPFSYILTV
                     AILSLFFKQNILCQIMGGGLILGAFFMATDYVTSPITKKGKIIFGIGCGIITFLIRKA
                     GGYPEGVCYSILFMNSLVPMINRYTVPKKFGSKK"
     gene            1861..2403
                     /locus_tag="PLF90_06945"
     CDS             1861..2403
                     /locus_tag="PLF90_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOK57049.1"
                     /translation="MKKKDIIIGILVITFICGISGFLLAQVYNTTKPKIEEIKKQEEE
                     KLNKEIFPEGVKFEEKEIKGIKYFSVLNKNEEEIGKIFEVKTMGYGGYITVKVGIDNE
                     NKIKKIKIKEHNETPGLGSKITQDSFLNQFTGKTKDEIYLKKDKTDGKIDSITGATIS
                     SRAVTDGVRKLLENIAKESK"
     gene            2400..2969
                     /locus_tag="PLF90_06950"
     CDS             2400..2969
                     /locus_tag="PLF90_06950"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOK57050.1"
                     /translation="MKILSFLGSPRKDGNTAKVLGIVLEELNKNGHETEEIYLIDKKI
                     SGCIECYECQKIKDKPNCSIKDDMQELYEKILSSDCILITTPVFCWSFSWLIKSFIDR
                     TFCFGKYNEDGTYISLVENKKCGLIITAAGDEFEGADLVVESYSRMVEYHKMEDIGRI
                     VICNVLSEKDIIDNPEVKRKIKNFVENLK"
     gene            2966..3574
                     /locus_tag="PLF90_06955"
     CDS             2966..3574
                     /locus_tag="PLF90_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOK57051.1"
                     /translation="MKKIIDFKVFLNGIWRENPILFIMLGLCPVLAVSSSIKDALGMG
                     IAVIFVLTGSNFVISLIKNIVPSHVRIPVYIVVIATFVTITDYTLAAYSPQIHRNLGV
                     YLPLIVVNCIILGRAEGFASKNNVINSILDGLGMGLGFTLIIFVIATIREVLGNGTFM
                     GKALFGPDFSPFLIMIMPPGAFLVIGILIALKQKIEKGKKIC"
     gene            3568..4158
                     /locus_tag="PLF90_06960"
     CDS             3568..4158
                     /locus_tag="PLF90_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HOK57052.1"
                     /translation="MLNETNLFFIFLSAFLINNILLIRFLGLCSFIGISTNIKSSIGM
                     GIAVIFVTVMACSISWAVYNFLLLPFHLEYLRTTTFILTIASFVQFEEMIIRKKLPAL
                     YRAFGIYLPLITTNCAILASAFLGIDYKFSFIQNFVFSLGISAGYCFVIIVFATIREE
                     IELAPIPESFRGVPIAFILAALMSLAFLGFKGLFKL"
     gene            4158..5300
                     /locus_tag="PLF90_06965"
     CDS             4158..5300
                     /locus_tag="PLF90_06965"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOK57053.1"
                     /translation="MFFFFPVRDEYGVKRFPIVVTFIIFINCLIYFAFGFSPEYREIV
                     SKYGFIPANFSFKTLLTSMFLHGGLFHLGFNMWYLWLLGDNVEDRWGRINFLFFYLLS
                     GIFAGILYSWLIPEKFINIPVIGASGAISGILGAYAILFPKSRITFKYFIWAFIIFVR
                     WGEFEIYSYVWLFFWFFIQGLNTLFVSLYKVQSQVAYSAHFGGFLFGAIIGLGTKLYR
                     EAKYRENVKLGENMLLQLLGSTEKVLYNIEETAEIEKIKEKIKNTIYEDRYFGTQIYK
                     KGIEKYPDLCMDEKIQYEIADSFYRQGDYTSALIAYRNFILNYPLSKLADNALLNFGK
                     ICLLSGDYEKAKQAFLQIVIFYPYSDVYEESKYYLEKELPKLIQKF"
     gene            complement(5304..5795)
                     /locus_tag="PLF90_06970"
     CDS             complement(5304..5795)
                     /locus_tag="PLF90_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOK57054.1"
                     /translation="MDLLKILKERRSIRKYQKKEVPKDIIEDIIDCARFAPSAINIQP
                     WEFVIVTDKNMREKIAEITDYGKFIKESPVCIAVFCKDTKYYLEDGSAATTYILLAAK
                     AYGLGSCWVAGDKKFYAEKIRELLGVPEGYKLISLISIGYPDEEPKPKKRELKELIHW
                     EKW"
     gene            complement(5854..7056)
                     /locus_tag="PLF90_06975"
     CDS             complement(5854..7056)
                     /locus_tag="PLF90_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK57055.1"
                     /translation="MEKVRIGIIGCGGISKCHIDGVKILAENGCKEIEITALCDERIE
                     NAEEKKEMILEFQKNEPVIFTDYEEIISKKICDGFVLCLPHFLHHTIGLELLKNNYHI
                     MIEKPLALTLKAGKKLVNEAKKRNLVLSVAENVRRYLSPRSIKWVIENKKYLGDIRFA
                     LVNLVMNGPFDYTKYALKWRGIKVLSGGGMIMDSGHHFADMLLYLFGEVEDVYCEIKR
                     FDSRIIEEVPFLKKGRADVEDTWSAIIKFKSGIFINWVYSRSAPGFNLNFGMYSGENG
                     AIKDKGFVFHPFQNGGDIILNNEKVIKSEEIEKEFLNSLTEEEKEELFPYNCYDGFGL
                     EWIDFARGIKGERKVEIDGEDGLKIMALCESCYESDYLKSSVKYGDVYSGKIKNYQKE
                     INDFWKIK"
     gene            complement(7043..8407)
                     /locus_tag="PLF90_06980"
     CDS             complement(7043..8407)
                     /locus_tag="PLF90_06980"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOK57056.1"
                     /translation="MFFGISGLYFTDKDLDIEKQQLIDEGRDIGEIEKEFEEMKNFLK
                     NKNPLEYQEKIFFLFDKCQKLPQKKDYKYFEPIKFEDIKSEWNNNFFKIENKLTEKEI
                     KDKIYGGWLGRCAGCLLGKPVEGWSKKKIENFLKDTGQFPLRFYMRSDFNEEILEKYN
                     INRNAPFINNIKNMVEDDDINYTVLALTLIEKYGFNFSAIDFAYNFLQKLPVLRTCTA
                     ERITYKNLVNLISPDYSALFRNPYREWIGAQIRTDFYGYITPGNPEKGVELAYKDASV
                     THIKNGVYGAMFITAMISIAFYEKDIKKIVKNALSFIPQKSRLAEGIEEILNYKEKNL
                     SYEEVVNIIHNKWNEKNPHHWCHVISNAQIVVIGLLWGNKDFGDSVCKAVNAGFDTDC
                     NGATVGSILGVLFGKKNLPSEWIKVLNNKVETGVSGYSKAKISELAKKTYDIYKKGEK
                     DGKS"
     gene            complement(8411..>8991)
                     /locus_tag="PLF90_06985"
     CDS             complement(8411..>8991)
                     /locus_tag="PLF90_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK57057.1"
                     /translation="WFTSKKLAGGGPMMDVGVHMIDMVLYIAGLTDPKIVFGATFEKF
                     KDKAVDGGWPPAETRKGDKPTGKMEVEDLANGFVKFSNGAVLFVEASWAGNSEVGLKG
                     SLFGTKAGAQLPDPLDNKNPVRIYTETNGVVSDIIPEIPKIDTYFEEVKHFIECIREG
                     KQPITKKEEIIGVVKIIEGIYKSAEKNGPIIF"
BASE COUNT         2960 a         1363 c         1290 g         3378 t
ORIGIN      
        1 taagagaagc aggtattgta ggtcttggtg gtgctgcttt tccaactcat gtaaaactaa
       61 caattcctga agggaaaaaa gcagattata tattaataaa tggctgtgaa tgtgaaccat
      121 ttttaacctg tgattacaga gttttaaagg agtatacaga agaggtcgtt gagggtattc
      181 aaataatatc taaggtaaca aatgtaaaaa aagtatttat aggtattgaa agaaataaaa
      241 ttgacctttt acctgtcttg aatgatgcta ttaagaaatt aaaaatatat attgaaattg
      301 aaataaaaat tctgcctgaa aaatatccac agggaagtga aaaacatctg attaaatcaa
      361 ttttaaataa agaagtccct tcgggtggat tgccgattga tgttgggtgt attgttttta
      421 atgttcaaac aactcttgca attaaaaggg ctgtatgtga tggaattcct ctaactgaaa
      481 gagttttaac atgtacagga cttgttgata atccaaaaaa tttaaaagtc aaaataggaa
      541 cgcctatttc agaaattatt gattattgta aaggtgatat taaaaatgga agcaaaattg
      601 ttgtaggtgg acctatgatg ggaatacaaa taccaaatcc tgatgttcct gttataaagg
      661 gtacaactgg aattttattg ttacctgaag aaaaactgcc tgaagatata caaccatgta
      721 taagatgcgg aaaatgtata gaagtatgtc ctatgttttt aatgccgagc gaaattggaa
      781 ggtatatgga atttgagaaa gttgaaaaat gtgaaaattt aaatgttttt gactgtattg
      841 aatgcggatg ctgtgcgtat atatgtcctg caaaaagacc gcttgttgat tttattaaat
      901 gtgctaaatt caaattgagg aaaaagtaaa tatggaaaaa gtttgtaaaa taaattgttc
      961 tcctcatatt catagcagtg aaacaacagg aaagataatg ttttatgtgg taatttcttt
     1021 aatccctgct cttattggaa gtatatattt ttttgggatt tatgtgcttg aagttatcat
     1081 tatatccata atttcatgca tagtgagtga acttttattc aaaattctaa ccagaaagaa
     1141 aatagaagtt tttgatggaa gtgctattgt aacaggtatt cttcttgctt ttgttttacc
     1201 accgagaatt cctttatgga ttgctggaat tggtggtttc cttgcaattt ttttggttaa
     1261 agaattattt ggtggaatcg gttttaacat tttcaatcct gcacttgcaa gtagagccat
     1321 tttacttgct tcatatcctg ttgaaatgac aaagtttatt cagccatttg attacaggat
     1381 agatgctata acttctgcaa cccctttatt tataattaaa gaaaaactta atcaacaact
     1441 tccttcttta tggcaaatgt ttattggaac aagacctggt tgtataggag aaacaagtac
     1501 attattatta cttattggtg gatttttcct tatttataaa agggtaattt cctggcatat
     1561 tcctttttcc tatatcctta cggttgctat tctatctctt ttcttcaaac agaatatttt
     1621 atgtcaaata atgggtggtg gtcttattct cggtgctttt ttcatggcaa ctgattatgt
     1681 tacctctcca ataacaaaaa agggtaaaat aatatttgga attggttgcg gaattataac
     1741 atttttgata agaaaagcag gaggttatcc agaaggtgtg tgttattcaa ttttatttat
     1801 gaattcactt gttcctatga taaatagata tactgtacca aaaaaattcg ggagtaaaaa
     1861 atgaaaaaaa aggatattat aatcggcatt ttagttatta catttatctg cggaatttca
     1921 ggatttcttc ttgctcaagt ttacaataca acaaaaccaa aaattgaaga aataaaaaaa
     1981 caggaagaag aaaaattgaa taaagagatt tttcctgaag gtgtaaaatt tgaagaaaag
     2041 gaaattaaag gaattaaata tttttctgtc ttaaataaaa atgaagaaga aattgggaaa
     2101 atatttgaag taaaaacgat gggttatggt ggatacatca cagtaaaagt tggaattgat
     2161 aatgaaaata aaattaaaaa gataaaaata aaagaacata atgaaacacc aggacttggt
     2221 tcaaaaataa cacaggatag ttttttaaat caatttacag gaaaaacaaa agacgaaatt
     2281 tatttgaaga aagacaaaac agatggaaaa atagattcaa ttacaggagc aactatttct
     2341 tcaagagcag taacagatgg agtcagaaaa ttacttgaaa atattgcaaa ggagtcaaaa
     2401 tgaaaatatt atcttttctt ggtagtccaa ggaaagatgg aaatactgca aaggtcttgg
     2461 gtattgttct tgaagagttg aataaaaatg gacatgaaac agaagagatt tatttaattg
     2521 ataaaaaaat aagtggatgt attgaatgtt atgaatgtca gaaaattaag gataaaccaa
     2581 attgttctat aaaggatgat atgcaggaat tatatgaaaa gattttaagt tctgattgta
     2641 ttttaattac aactcctgtt ttctgctggt ccttttcgtg gcttattaaa tcatttattg
     2701 accgaacatt ctgttttggt aaatataatg aagacggaac ttatatatca cttgttgaaa
     2761 ataaaaaatg cggtcttatt attacagcag caggagatga atttgaaggt gctgaccttg
     2821 tggttgaaag ttattcaaga atggttgaat atcataaaat ggaagatata ggaagaattg
     2881 ttatttgtaa tgttctatca gaaaaagata ttattgataa tcctgaggtt aagagaaaaa
     2941 ttaaaaattt tgtagaaaat ttaaaatgaa aaaaataatt gattttaaag tttttttaaa
     3001 tggaatttgg agagaaaatc caatactttt tataatgctt ggattatgtc ctgttcttgc
     3061 tgtaagtagt tctataaaag atgctcttgg tatgggaatt gctgtcattt ttgttttaac
     3121 cggctcaaat tttgtaattt ccttaataaa aaatatagtt ccttcacatg taaggattcc
     3181 tgtatatata gttgttatcg ccacatttgt tacaattact gattacacac ttgctgcata
     3241 ttctccacag atacacagaa atcttggagt atacttgcct ttaatagttg ttaactgtat
     3301 aattcttggc cgtgcagagg gttttgcttc aaaaaataat gttataaatt ctattcttga
     3361 tggtctcggt atgggactcg gttttacttt aataattttt gtaattgcaa ctataagaga
     3421 agtgcttgga aatggaacat ttatgggaaa ggcattattt ggacctgatt tcagtccatt
     3481 tttaattatg attatgccac caggagcttt tttggtaata ggaattttaa ttgctttaaa
     3541 acagaaaatt gaaaaaggaa agaaaatatg ttaaatgaga caaatttatt ttttattttt
     3601 ctttctgctt ttttaataaa taatatttta cttataagat ttctcggact ctgctctttt
     3661 attggtattt caacaaatat aaaatcatca attggaatgg gtattgctgt tatttttgta
     3721 actgttatgg catgcagtat aagttgggct gtttataatt ttttactttt accttttcat
     3781 cttgaatatt taaggacgac tacttttata ctaacaattg cttcatttgt tcagtttgag
     3841 gaaatgataa taagaaaaaa attacctgcc ttatatcgtg catttggcat ttatctccct
     3901 ttaattacaa caaattgtgc aattttagca agtgcttttc ttggaattga ttataaattt
     3961 tcctttatac agaactttgt tttttctctc gggatttctg ctggatattg ctttgtaata
     4021 attgtttttg ctactataag agaagaaatt gaacttgcac ctattccgga aagttttaga
     4081 ggagtaccca ttgcttttat tcttgctgcc ttgatgagtt tagcatttct tggttttaag
     4141 ggcctattta aactataatg tttttctttt ttcctgtcag ggatgaatat ggtgttaaaa
     4201 ggtttccgat agtagtaact tttataattt ttataaactg ccttatatat tttgcctttg
     4261 gtttttcacc tgaatacaga gaaatagttt caaaatatgg ttttattcct gccaattttt
     4321 ctttcaaaac tttacttact tctatgtttc ttcatggagg gctttttcat cttggcttta
     4381 atatgtggta tttatggctc cttggagata atgttgaaga tagatgggga aggataaatt
     4441 ttttattttt ttatcttctt tcgggtattt ttgcagggat tttatactca tggctaatac
     4501 ctgaaaaatt tatcaatatc cctgttatag gtgcatcagg tgcaatttca ggaattcttg
     4561 gtgcttatgc tattttattt cctaaaagca gaataacttt taaatatttt atatgggctt
     4621 ttataatatt tgtaagatgg ggcgaatttg aaatttattc atatgtgtgg ctttttttct
     4681 ggttttttat acagggatta aacactttat ttgtttcgct atataaagtt caatctcagg
     4741 ttgcttattc tgcacatttt ggtggctttc tattcggagc aattattgga cttggaacaa
     4801 aactatatag agaagcaaaa tacagagaaa atgtaaaact tggagaaaat atgcttcttc
     4861 aacttcttgg ttcaacagaa aaagttttat acaatataga agaaactgct gaaatagaaa
     4921 aaattaagga gaagataaaa aatacaattt atgaggatag atattttgga actcagattt
     4981 ataaaaaagg aatagaaaaa tatcctgatt tatgtatgga tgaaaaaatt cagtatgaaa
     5041 ttgctgattc cttttacaga cagggtgatt atacttctgc tttaattgct tatagaaatt
     5101 ttattttgaa ttatccttta agcaaacttg ctgataatgc tcttttaaac tttggtaaga
     5161 tatgtctcct gtcaggtgat tatgaaaagg caaaacaggc attcttacag atagttattt
     5221 tttatccata cagtgatgtg tatgaggaaa gtaaatatta tcttgaaaaa gaacttccaa
     5281 aattaataca gaaattctga tttttaccat ttttcccaat gtattaattc tttcaattct
     5341 ctcttttttg gtttaggttc ttcatcagga tatcctattg aaattaaact tataagtttg
     5401 tatccttcag gaactccaag taattctcta atcttttctg cataaaattt tttatctcca
     5461 gcaacccagc aacttccaag tccatatgct tttgcagcaa gtaaaatata tgtagtagca
     5521 gcagaaccat cttcaagata atattttgta tctttacaga aaactgcaat acaaacagga
     5581 gattccttta taaatttccc gtaatcagta atttctgcta tcttttctct catgtttttg
     5641 tccgtaacaa ttacaaactc ccatggctgg atgttgatag cgcttggagc aaatctggca
     5701 caatcaatta tatcttcaat aatatctttt ggaacttcct ttttttgata cttccttata
     5761 cttcttcttt cttttaaaat tttcaataaa tccatacctt cccccttttt taatctggaa
     5821 aattaagttc tgtctgttat ttatgttttt catttatttt attttccaga aatcattaat
     5881 ttctttctgg taatttttaa tttttccaga ataaacatcc ccatatttta cagatgattt
     5941 taaataatcg gattcataac aactttcaca taaagccata attttcaaac catcttctcc
     6001 gtcaatttca acctttcttt ctccttttat ccctcttgca aaatcaatcc attcaagacc
     6061 aaatccatca tagcagttat aagggaaaag ttcttctttt tcttcttctg ttaaactatt
     6121 taaaaattct ttttctattt cttcactttt tattactttt tcgttattca aaattatatc
     6181 tccaccattc tggaaaggat gaaaaacaaa tcctttatct tttatggctc cattttcacc
     6241 tgaatacatt ccaaaattta aattaaatcc aggtgcagac cttgaataaa cccagtttat
     6301 aaaaatcccg cttttaaatt ttatgatagc actccatgta tcttctacat ctgccctacc
     6361 cttttttaaa aatggaacct cttctattat tcttgaatca aatctcttta tttcacaata
     6421 tacatcttca acttctccaa aaagatataa aagcatatca gcaaaatgat gaccgctatc
     6481 cataatcatt ccaccaccac ttaaaacttt tattcctctc cattttaaag catatttggt
     6541 ataatcaaaa ggtccattca taactaaatt tacaagagca aaccttatat cacccaaata
     6601 ttttttattc tcaataaccc attttatact tcttggagat aaatatcttc ttacattttc
     6661 agcaactgat aaaaccagat tccttttttt tgcttcattc acaagtttct ttcctgcttt
     6721 taaagtcaaa gcaagtggtt tttctatcat aatatgataa ttgtttttga gaagttcaag
     6781 tcctattgta tgatgaagaa aatgaggaag acataaaaca aatccatcac atattttttt
     6841 tgaaattatc tcctcgtaat cagtaaaaat aactggttca tttttttgaa actctaaaat
     6901 catttctttt ttttcttctg cattttctat tctttcatca caaagtgcgg ttatttctat
     6961 ttctttacaa ccattttcag caagtatttt caccccatca atatgacatt tacttatccc
     7021 tccacaacca attatcccaa ttctaacttt ttccatcttt ttctcctttc ttataaatat
     7081 catatgtttt ttttgccagt tctgaaattt ttgctttact ataacctgaa actcctgttt
     7141 ctactttatt attcaaaacc ttaatccatt cagagggtaa atttttcttt ccaaacaata
     7201 ctccgagaat tgaaccaaca gttgctccat tacaatcggt gtcaaatcca gcatttactg
     7261 ccttacatac tgaatctcca aaatctttat taccccatag aagaccaatt acaacaatct
     7321 gagcattact tattacatga caccagtgat gtggattttt ctcattccat ttattatgga
     7381 taatatttac aacttcttca tatgaaagat ttttttcttt ataatttaat atttcttcta
     7441 taccctctgc taatcttgat ttttgaggta taaaactcaa tgcatttttt acaatttttt
     7501 taatatcttt ttcataaaaa gcaattgata tcattgcagt tataaacatt gctccataaa
     7561 caccattttt tatatgagtt acagaagcat ctttatatgc aagttcaacc cctttttcag
     7621 gatttccagg tgttatatat ccataaaaat cagttcttat ctgtgcacct atccattctc
     7681 tgtaaggatt tctgaataaa gcagaataat caggagaaat aagatttaca agatttttat
     7741 atgtaattct ttctgcagta catgttctta aaacaggaag tttttgtaaa aagttataag
     7801 caaaatcaat tgctgaaaaa ttaaagccat atttttctat caaagtaaga gcaagaacag
     7861 tatagtttat atcatcatcc tcaaccatat tttttatatt attgataaaa ggagcatttc
     7921 tgttaatatt atatttttca agtatttctt cattaaaatc acttctcata tagaatctta
     7981 aaggaaactg tcctgtatct tttaaaaaat tttcaatttt ctttttactc catccctcaa
     8041 ctggttttcc aagaagacat ccagcacatc taccaagcca gccaccataa attttatctt
     8101 ttatttcctt ttcagtaagt ttattttcaa ttttaaaaaa attattgttc cattcgcttt
     8161 ttatatcttc aaattttatt ggttcaaagt atttataatc ctttttctgt ggtaatttct
     8221 gacatttatc aaaaagaaaa aatatttttt cctgatattc taatggattt ttgtttttca
     8281 aaaaattttt catctcttca aattcttttt ctatctctcc aatatctctt ccttcatcaa
     8341 ttaattgctg tttttcaata tctaaatctt tatctgtgaa atataaacca cttattccaa
     8401 aaaacatttt ttaaaatata ataggtccgt ttttctctgc ggatttatat ataccctcaa
     8461 ttattttaac tacacctatt atttcttcct tttttgtaat tggctgtttt ccttctctaa
     8521 tacactcaat aaaatgttta acctcttcaa aatatgtatc tatttttgga atctcaggga
     8581 taatatcact tactactcca ttcgtttcag tataaattct gactggattt ttattatcaa
     8641 gagggtctgg taattgtgca cctgcttttg ttccaaataa actaccttta agtccaacct
     8701 cagaatttcc agcccaactt gcctctacaa aaagaaccgc tccatttgaa aatttaacaa
     8761 aaccatttgc caaatcctct acttccattt ttcctgtagg tttatctcct ttccttgttt
     8821 cagcaggagg ccatccaccg tcaactgctt tatccttaaa cttttcaaat gtcgctccaa
     8881 aaactatttt tgggtctgtt aaccctgcaa tatataaaac catatctatc atatgaactc
     8941 caacatccat cataggacct ccaccagcaa gttttttact tgtaaaccag g
//
LOCUS       DAOSAT010000074         8828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_5177_length_8828_cov_5.477830, whole genome shotgun sequence.
ACCESSION   DAOSAT010000074 DAOSAT010000000
VERSION     DAOSAT010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5177_length_8828_cov_5.477830"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PLF90_06990"
     CDS             <1..506
                     /locus_tag="PLF90_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOK57058.1"
                     /translation="NRGYVFVGTAKEKVAAPVVEKKAEPPVKVVEKIVEKPVEKIVTK
                     IVEKPVEKIVKVPGKTLVINLQDVYFNYDSAAITPLAAQTLKENASVLKANPSVNVLL
                     VGSASPEGATDYNLKLSERRVNAVKDYLIKEGISADRLKTNAAGEIEVEKPSWPFARK
                     VKFAVAE"
     gene            557..982
                     /locus_tag="PLF90_06995"
     CDS             557..982
                     /locus_tag="PLF90_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HOK57059.1"
                     /translation="MLISMLLTGCETIQSLSPETKKGAAIGAAAGGILGALLDSSKPW
                     RGAIIGAAVGAAAGGWIGYKLEGKEAVAQADEDVVSKAAKDAATLNSTVKYSRVTEAG
                     VTEEIIATPGELKGNIRKVTIQYFRNGTLISTETREIQV"
     gene            1256..4624
                     /locus_tag="PLF90_07000"
     CDS             1256..4624
                     /locus_tag="PLF90_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOK57060.1"
                     /translation="MNNFITNSDTKILKDRLNELILKSEELKFLVGFFYFSGIRELFE
                     SIQKNSNVTLKILVGLNVDKTSYGLIEYPENAPLSDEEKINNFFDSIKKSINTTDFDK
                     KDFYEQARFFIQLVEEGRLIIRKTVDPNHAKLYLFKLNPTQVSRRNLFITGSSNLTRS
                     GLVEQSEFNVEISDYGVDNAEKYFDDLWKGGNSIEITENPETRRKLIDVLKNETLIRE
                     ITPFEAYVYVLKNYLDTFEKKEETTSLEELLIKLGYKSFKYQVDAVEQALAIIEQNKG
                     VILADVVGLGKTIIACLIARELRKRGLVICPPGIIGDEKEEKSGWKWYIEQFGLYDWI
                     VESVGKLDDPEFAKSINKLKGIEVVIVDEAHRFRNEDTKSYEQLKNICRNKIVILLTA
                     TPFNNRPADILSLLKLFIVPKKSVISLENNLVDRFRIFKSSFDKLGYIRKYYNSKDME
                     KREKAISYYNSMFGEGDVDLKKVKQRTGYIAKQIKSVIEPVTIRRNRLDLQKNPYYKE
                     EVKELTKVANPREWFFELTKEQSKFYDEVITKYFTYPEDNGLFKGAIYRPFEYKIDRK
                     QIEKLSEEDNRRYQQQRNLYDFMRRLLVKRFESSFGAFRQSIENFKNVTEHCEKFIGK
                     TNKFILDRSLLEKIYQLELDEIEKYLMEYAEKIKDGVYPKNHEIYEVDEFKYKSEFLS
                     DIENDKRLFGSILEDLDKLKLVENDPKFKRLSNELKAHFKNEPERKIVIFSEYIDTIE
                     YLENHLKPYFNNRILVIKGSLNRYKIDEINENFDASYPKEKQKNNYDILLSTDRISEG
                     FNLNRAGMVINYDIPWNPVRVIQRVGRINRISKKVFDEIYIVNFFPTEKGADLVKSRD
                     IAATKMFLIHNTLGEDSKIFDIDEEPIPSELYKKIQQNPDEIESESFYTKVLNEYEDI
                     KKQFPGIIERIEDFSPRVKVSKKFNEGELLVFIKKNKRFFTQRIRYNQEKIEPETVVI
                     EDIIERIKTTQEEPSLPLSDKFWDAYEKIKTYKEESMSIPEQSLEKKALHNVKTLLHI
                     ENENLTPLRNFLNMIRKDILDYGTLSDYTLRRIANLEIFDKNRNLDEEKLKKTIEEVK
                     IIKEELGEDYLIKEKLKLQQLHKEIIIAIENREK"
     gene            4621..8178
                     /locus_tag="PLF90_07005"
     CDS             4621..8178
                     /locus_tag="PLF90_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HOK57061.1"
                     /translation="MSNEILKDIINDFSVDKFIRFFRLKTKKFSLNREELLKYNNDLF
                     TEGSKLGEINFEYDKLIICAFRVKKELSERSGKKAQYEVGKKILKDTQSDAGIFIFYD
                     KNRNFRFSLIFTEYFGINREFSYFKRFTFYVSDCLTNKTFIQQIGSCNFSSLEEIKQA
                     FSIEPVTKHFYEEIQNWYFWAMDEVKFPEDFKCSDRPEKDKKIRNATNLIRLLTRIIF
                     IWFIKEKKLIPEILFDKSELKEIVKDFGNGNNYYNAILQNLFFATLNSKMDERKFAED
                     DGFLTNRKEYGVKILYRYADKFLIPKQDVLNLFKDIPFLNGGLFDCLDKEDENGTVIY
                     IDGFSRNPKKQAVVPDYLFFKEKEEKVDLSQYGIGSDKLVRGLIEILKSYNFTIDENT
                     PIDQEVALDPELLGKVFENLLASYNPETATTARKATGSYYTPREIVDYMVEESLFEYL
                     KTKFPEISEDEIRLLLSYSDGIPELTEEEKQKIITVIGNIKILDPACGSGAFPMGILH
                     KLVHILTKLDPDNKYWHEIQYQKALKASEEVFKERNNKAEREELLKEINDAFDENINY
                     PDYARKLYLIENCIYGIDIQPIAVQISKLRFFISLVLDQKVDRGKENLGVRPLPNLET
                     KFVAADTLIALESPESPRLKDPDIKEKEEQLEELRHKYFTAKTRTQKKRIQKKYKKLR
                     EKIAKLLIKDRWGEQTVKKIASFDPFDQNTFADWFDPKWMFGVVDGFDIAIGNPPYVR
                     QEKIKPLKHSLKEAYKDFFNSTSDLYIYFYKKSYDILRNHGILCFISSNKWMRAKYGE
                     KLRKFLKENTKVKELIDFSGYSVFEQTVDTNIIIFQKENPGKVHALYFVNVKSDVDDV
                     ISYIQNNKRILSQQKLSDNAWTLADERVLALKEKIEKIGKPLKDWDVKIYFGIKTGFN
                     EAFIIDTKTRDGILANCKTQEERKRTEEIIKPILRGRDIGRYYYKWAGLWVIKIESGW
                     TNKNRRNEKPEEFFKKMSPSIYRHLISFANKEVEGSRKGLLNRDDQGDYWWELRNCAY
                     YPEFEKEKIVWQEIVREPSFAYDNTGIYCEATSFLMTGKNLKYIIGLLNSKPVMFFFR
                     NYYAGGGLGEEGYRYKKAFLKQLPLPPITPQNQPLVTQIESLVSQILNVDTDKNVCAT
                     TNQQKQAKVKELEKQIDQLIYKLYDLTEEEIKIIEKGDK"
     gene            8265..8777
                     /locus_tag="PLF90_07010"
     CDS             8265..8777
                     /locus_tag="PLF90_07010"
                     /inference="COORDINATES: protein motif:HMM:NF027286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HOK57062.1"
                     /translation="MEYYSGVWNFFVLLISASLLLWQIISQNIWIRKKNSLELINQIF
                     KGDMLKVKSEVDKYLRKSSNFLNETYKEIADNIHKEEKEKLDECLQIYLNYLEGIALG
                     IKYKVYEEQMLYDYIESIITQIYRWAKPYIEEMRKKFGDKELYIELEKLAEKWNKIKQ
                     NRKEKYKNAF"
BASE COUNT         3542 a         1139 c         1588 g         2559 t
ORIGIN      
        1 gcaacagagg ttacgtcttc gtaggaacgg caaaagaaaa agtggcggca cctgtggttg
       61 agaaaaaggc agaaccacct gtgaaggtag tggaaaaaat agtggaaaaa cctgtagaga
      121 aaatagtaac aaaaatagtg gaaaaacctg tagagaaaat agtgaaggtg ccgggaaaga
      181 ctcttgtaat caatctccag gatgtgtatt tcaactatga tagtgctgca atcacacctc
      241 ttgctgcaca gacattaaag gaaaatgcat ctgtccttaa ggcaaatcca tctgtgaatg
      301 tactgcttgt tggctctgca tcacccgaag gtgctactga ttataacctg aaactttctg
      361 agcggagagt aaatgcagtg aaggactatc taataaagga aggtatcagt gcagacaggt
      421 taaagacaaa tgcagcagga gagattgaag tagaaaaacc gtcctggcca tttgccagaa
      481 aggtgaaatt cgcagttgcc gaataaactc tgtatgtaaa aaggagttca taatgaaaaa
      541 attagtggtg gggttgttgt tgataagtat gctattgacc ggctgcgaaa ctatccagtc
      601 actttctcct gagacaaaaa agggtgctgc aataggtgcg gcagcaggtg gaatactggg
      661 ggcattactt gattcaagta aaccatggag aggtgctatt ataggtgctg ctgtaggtgc
      721 agcagcaggc ggatggattg gctataaatt agaaggtaaa gaggcagttg cacaggcgga
      781 tgaagatgtt gtaagcaaag cagcaaaaga tgcagcaact ctcaattcca ccgtaaaata
      841 ctcaagggta acagaggcag gtgttacaga agagattatc gccactccag gagaactgaa
      901 aggcaatata aggaaagtca ccatccagta tttcagaaac ggcaccctga tatccactga
      961 aacacgagag attcaggtat aagaaaaaga ggcaggtaga aagattactc tttttacctg
     1021 cctttttctg caagatgggg gtcaggtctt gctttttgca tctcttatag ggaagaatat
     1081 atttcgtctc ttaataactt aacagaaaca gaatacagta ttgctatttg ccattcttct
     1141 gaggagaact tttctgagag acctatacaa cctcatagag attatctttg aggaaggtcg
     1201 gacattccct tttttactaa gactatgata aaatattaac taaaaaacaa aagaaatgaa
     1261 taattttata acaaatagcg acacaaagat attaaaagat aggttaaatg aattaatatt
     1321 aaaaagtgaa gaattaaagt ttttagttgg ttttttttat ttttctggca ttagagaatt
     1381 attcgaatct attcaaaaaa attctaatgt aactctaaaa attcttgttg gtttgaatgt
     1441 agataaaaca tcctatggat taatagaata tccagaaaac gcccctcttt cagacgagga
     1501 aaaaatcaat aacttctttg attcaattaa aaaatctata aatactacgg attttgataa
     1561 aaaagatttt tatgaacaag caaggttttt tatacagcta gttgaagaag gtcggcttat
     1621 aattagaaaa actgtagacc ccaaccatgc aaaactgtat ctttttaaat taaatccaac
     1681 ccaagtgagt agaagaaacc tttttatcac tggaagtagt aatttgacac gttcaggatt
     1741 agttgaacaa tcagaattta atgttgaaat aagtgattat ggcgttgata atgcagaaaa
     1801 gtattttgat gatttatgga agggtggtaa ctcaattgaa ataactgaaa acccggaaac
     1861 aagaagaaaa ttgatagatg ttttaaaaaa cgaaacatta ataagagaaa taactccttt
     1921 tgaagcatat gtatatgtgc ttaaaaacta cttagacaca tttgaaaaaa aagaagaaac
     1981 tacatcatta gaagaattac ttataaaact tggttataaa tcctttaaat atcaggtaga
     2041 tgctgtagaa caagcacttg caataattga acaaaataaa ggagttattt tggcagatgt
     2101 tgtaggtctt ggaaagacaa ttattgcttg tttaatcgcg agagaactga gaaaaagagg
     2161 attagttatt tgtcctcctg ggattattgg ggatgaaaaa gaagaaaaat ctggttggaa
     2221 atggtatatt gaacaattcg ggctttatga ctggatagta gagtctgtag gtaaattaga
     2281 cgacccagaa tttgcaaaat ctattaataa attaaaaggg attgaagtag tgattgtaga
     2341 tgaagcccat agatttagaa acgaagatac aaagtcgtat gaacagttaa aaaatatatg
     2401 caggaataaa attgttatac ttcttaccgc cacacctttt aataatagac cagcagatat
     2461 tctttctttg ctaaaattgt ttatagtacc taaaaaatct gttatttcac ttgaaaataa
     2521 tttagttgat agatttagaa tttttaaaag ttcatttgat aaacttggtt acattagaaa
     2581 atattataat tcaaaagata tggaaaaaag agaaaaagcc atcagttatt ataattctat
     2641 gtttggtgaa ggagatgttg atttaaaaaa agtaaaacaa agaacaggtt atatcgcaaa
     2701 acaaataaaa agtgtaattg aaccggtaac aattagaaga aaccgacttg accttcagaa
     2761 aaatccctat tataaagaag aggtaaaaga attaacaaaa gtagcaaatc caagggaatg
     2821 gttttttgaa ttaacaaaag aacaatcaaa attttatgat gaagtaatta ctaaatattt
     2881 tacttaccct gaagataatg gtttgtttaa aggagcgatt tatagacctt ttgaatacaa
     2941 aattgataga aaacagatag agaaattgtc agaagaggat aatagaagat atcaacagca
     3001 aagaaactta tatgatttta tgagaaggtt gcttgttaag agatttgaaa gttcttttgg
     3061 tgcgtttaga caaagtatag aaaattttaa aaatgttaca gaacattgtg aaaaatttat
     3121 tggaaaaaca aataagttta tacttgatag aagtttactt gaaaaaatat atcaactgga
     3181 attagatgag atagaaaaat atttaatgga atatgctgaa aaaattaaag atggtgttta
     3241 tcccaaaaat catgaaatat acgaggttga tgaatttaaa tacaaaagtg aatttttgag
     3301 cgatatcgaa aatgataaac gactttttgg atctatttta gaagaccttg ataaattaaa
     3361 gttagtagaa aacgacccca aattcaaacg tttatcgaat gaattaaaag cacattttaa
     3421 aaatgagccg gaaagaaaaa ttgtaatatt ttctgaatat attgatacaa ttgaatatct
     3481 tgaaaatcat ttgaaacctt actttaataa tagaattctg gttataaaag gttctttgaa
     3541 tagatataaa atagatgaaa ttaacgaaaa ttttgatgct tcttatccta aggaaaaaca
     3601 aaaaaataat tatgatatac ttctttctac agacagaatc tcagaaggat ttaatttaaa
     3661 ccgggctggt atggtaataa actatgatat cccttggaat cctgtgaggg ttattcaaag
     3721 agtaggaagg attaatcgta tcagtaagaa agtttttgat gaaatatata tagttaattt
     3781 cttcccaact gaaaaaggag cggatctggt aaaatccaga gatatagcag caaccaaaat
     3841 gtttctaatc cataatacac ttggggaaga ttcaaaaata tttgatatag atgaagaacc
     3901 aataccctct gaactataca agaagattca acaaaaccca gatgaaatag aatcagaaag
     3961 tttctatact aaagttttaa atgaatatga agatataaaa aagcaattcc ctggaataat
     4021 tgaaaggata gaagattttt cgccaagggt aaaagtatct aaaaagttta atgaaggtga
     4081 gttattagta tttataaaga aaaataaaag attttttact caaaggataa gatataatca
     4141 agaaaaaata gaacctgaaa cagtagtaat tgaagatata attgaaagaa taaaaactac
     4201 acaagaagaa ccttctcttc cattaagcga taaattctgg gatgcttacg aaaaaattaa
     4261 aacttataaa gaagaaagta tgtcaattcc tgagcagagt ttggaaaaaa aggcattaca
     4321 taatgtaaaa actttacttc atatagaaaa tgaaaattta actccattaa gaaatttttt
     4381 aaatatgatt agaaaagata ttcttgatta tgggactttg tctgactata cccttagaag
     4441 aattgcaaat cttgaaattt ttgataaaaa taggaattta gatgaagaaa aattgaaaaa
     4501 aacaattgaa gaagtaaaaa taataaagga agaactgggt gaagattact taataaaaga
     4561 aaaattgaaa ctgcaacaac ttcataaaga aataataatt gcaattgaaa acagagaaaa
     4621 atgagtaacg aaattttaaa agatataata aacgattttt cagttgataa gtttatacgc
     4681 tttttcagac ttaaaactaa aaaattttca ctaaatagag aagagcttct gaagtataac
     4741 aatgaccttt ttacagaggg ttctaaactt ggagaaatta attttgaata tgataaactt
     4801 attatctgtg cttttagagt caaaaaagaa ctgtcggaac gttcaggcaa aaaagcccag
     4861 tatgaagttg gtaaaaaaat tcttaaggac acgcaatcag atgctggaat atttattttc
     4921 tatgataaaa atagaaattt tagattttca ttaattttta ctgaatactt cggaataaac
     4981 cgagaattca gttatttcaa aaggtttact ttctatgtaa gcgattgttt aacaaataag
     5041 acatttatcc aacagattgg gagttgtaat ttttcatctc ttgaagaaat aaaacaggca
     5101 ttttctattg agccagttac aaagcatttt tatgaggaaa ttcaaaattg gtatttctgg
     5161 gcgatggatg aggttaaatt tccagaagat tttaaatgta gtgatagacc tgaaaaagat
     5221 aagaagattc gtaatgcaac caatctaatt cgtttactca cccgtatcat atttatctgg
     5281 tttataaaag agaagaaact tataccagaa attctctttg ataaatccga gttaaaagaa
     5341 atagtcaaag attttggtaa tggaaataac tattataatg ctattcttca gaatttattt
     5401 tttgcaacat taaatagtaa aatggacgaa agaaaatttg cagaagatga tggattttta
     5461 actaatagaa aagaatatgg ggttaagata ctttatcgct atgctgataa gtttttaatc
     5521 ccaaagcagg atgtcctaaa tctttttaaa gatattcctt ttttaaatgg tgggcttttt
     5581 gattgtcttg acaaagaaga tgaaaatggg acggttattt atatagatgg attttcaaga
     5641 aacccaaaga aacaggcagt tgtccctgac tatcttttct ttaaggaaaa agaagaaaaa
     5701 gttgaccttt ctcaatatgg cattggttca gacaaattag taagaggact tattgaaata
     5761 ctcaaaagtt ataactttac aatagatgaa aatacaccca ttgaccaaga agtagcactt
     5821 gaccctgaac ttttaggaaa agtttttgaa aatttacttg cttcttataa tcctgaaaca
     5881 gccacaacag caagaaaagc aacagggagt tattatacac caagagaaat agttgattat
     5941 atggttgaag aaagtttgtt tgaatactta aaaacaaaat ttccagaaat aagtgaagat
     6001 gaaattcgtc ttctcctttc ttattctgat ggaattcccg aacttaccga agaagaaaaa
     6061 caaaaaataa ttactgttat tggtaatatt aagatacttg accctgcctg tggttctggt
     6121 gctttcccta tgggaatact acataaactt gtgcatatac taacgaaatt agaccctgac
     6181 aataaatact ggcatgaaat ccaatatcaa aaagcattga aagcatcaga ggaagttttt
     6241 aaagaacgga ataataaagc agagagagaa gaacttttaa aagaaatcaa tgatgcattt
     6301 gatgaaaata taaactatcc tgattatgcg agaaaacttt atctgataga aaactgtata
     6361 tatggaattg atattcagcc aatagcagtt caaataagta aattaagatt ttttatatct
     6421 ttggttttag accagaaagt ggatagagga aaagaaaatt tgggtgtaag accacttcca
     6481 aatcttgaaa caaaatttgt agcagcagat acattgatag cacttgaaag cccagaaagc
     6541 ccacgattga aagaccctga tattaaggaa aaagaagaac aattagaaga attaagacat
     6601 aaatatttca ctgcaaaaac aagaacacag aaaaaaagaa tccaaaaaaa gtataaaaag
     6661 ttgagagaaa aaatagcaaa attacttatt aaagaccgct ggggagagca aacagtaaaa
     6721 aaaattgctt cctttgaccc ttttgaccag aatacttttg cagattggtt tgaccctaaa
     6781 tggatgtttg gtgttgtaga tggttttgac atagcgatag ggaatccgcc atatgtaaga
     6841 caagaaaaaa taaaaccctt aaaacattca ctcaaagagg catataaaga tttcttcaat
     6901 tccacatctg acctttatat atacttctac aaaaaatctt atgatatttt acgaaaccat
     6961 ggaatacttt gctttatctc aagcaataaa tggatgaggg caaaatatgg tgagaaatta
     7021 agaaaattcc taaaagaaaa tacaaaagta aaagaactca ttgatttttc tggttattca
     7081 gtctttgagc aaactgttga tacaaatata attatttttc aaaaagaaaa tccgggaaaa
     7141 gtccatgccc tatattttgt aaatgtcaaa agcgatgttg atgatgtgat aagctatatt
     7201 caaaataata aacgaatcct ctcacaacaa aaactctctg acaatgcctg gacactcgca
     7261 gacgaaagag tccttgcatt aaaagaaaag atagaaaaaa ttggaaagcc attaaaagat
     7321 tgggatgtga agatttactt tggaataaaa acaggtttta acgaagcatt tataattgat
     7381 acaaagacaa gagatgggat tttagcaaac tgcaagacac aagaagaaag aaaaaggaca
     7441 gaggaaatta ttaagccaat cttaagagga agggatattg gaaggtatta ttacaagtgg
     7501 gcagggttgt gggtgattaa aattgaatct ggctggacaa ataaaaatcg tagaaatgaa
     7561 aagccagagg aattttttaa aaagatgtcc ccgagtattt atcgacatct aatctctttt
     7621 gcaaacaaag aagttgaagg cagcagaaaa ggacttttaa atcgtgatga tcagggagat
     7681 tattggtggg aattaagaaa ttgtgcctac taccccgaat ttgaaaagga aaagattgtg
     7741 tggcaagaga tagttagaga accaagtttt gcttatgata atacaggaat ttattgtgaa
     7801 gcaacatcat ttttaatgac tggcaaaaac ttgaaatata ttatcgggct tttaaactct
     7861 aaacccgtta tgtttttctt tagaaattat tatgcaggtg gaggtttagg tgaagaaggt
     7921 tatagatata aaaaagcctt tttaaagcaa ctcccccttc ctcccattac cccacaaaac
     7981 caacccttag taacacaaat agaatccctc gtttcccaaa tcctcaatgt tgacacagac
     8041 aagaatgtct gtgctacaac aaaccaacaa aagcaggcaa aagtaaaaga acttgaaaaa
     8101 cagatagacc aactgattta taaactttat gatttaacag aagaggaaat aaaaataatt
     8161 gaaaaaggag ataaatgaaa aagaaagttt tctgtgttgg tctcataatt tttattttta
     8221 ttattctttt cctccttaac aaatcctctc aagttttcca gtcgatggag tattattcag
     8281 gggtttggaa ttttttcgtt cttcttatct ctgcttcact attactctgg caaattattt
     8341 ctcaaaacat ttggattaga aagaaaaatt cccttgaact tataaaccag atatttaaag
     8401 gagatatgtt aaaagtaaaa agtgaggttg ataaatattt aagaaaatca tcaaattttt
     8461 taaatgaaac atacaaagag attgcggata atatacataa agaagaaaaa gaaaaattag
     8521 atgaatgctt acagatttat ctaaactatc ttgaaggtat tgctcttgga ataaaataca
     8581 aagtttatga agaacaaatg ctttacgatt atatagaatc tattataact caaatatata
     8641 gatgggcaaa accttatatt gaagagatgc gaaaaaaatt tggtgacaaa gaactttata
     8701 tagaacttga aaaacttgct gaaaaatgga acaaaattaa acagaataga aaagagaaat
     8761 ataaaaatgc tttttaaaaa tgaacaagat ttaaaagact tcattaataa tcgggttatt
     8821 gaaaaaaa
//
LOCUS       DAOSAT010000075         8439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_5491_length_8439_cov_4.644442, whole genome shotgun sequence.
ACCESSION   DAOSAT010000075 DAOSAT010000000
VERSION     DAOSAT010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8439
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5491_length_8439_cov_4.644442"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="PLF90_07015"
     CDS             <1..121
                     /locus_tag="PLF90_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK57063.1"
                     /translation="ETNWNQTKAAEKLKLTRRVLRYKIQKYGLLKDERKNTDN"
     gene            96..1496
                     /locus_tag="PLF90_07020"
     CDS             96..1496
                     /locus_tag="PLF90_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK57064.1"
                     /translation="MKERILIIEDNREITELIVNILTLNKFIVDYVFSGNEGIEKTKK
                     ENYHLVILDLQLPDIDGFKVLEEIKKNNENLPVIILTGFGTTENIVKAIKIGADDFIE
                     KPFDINDFIEKVKKYIKLSSLEKELTEFRLIESILQLNRTIISLSDLDTLLNTVIQII
                     DNLFSPDRIGIYLYNSENKNYVLKKYKFKNLQQKIKIVYKDSEIKELFREKGFLIEEL
                     RDIHNLKILIKGKEKDIGIFEITFKEDRKVKEEEINFFEAFSIQIGIGIENALLLEML
                     QNSYINAISSLIMSLEAKDKYTKGHSEEVVYYSLLIGKKLGLKDEELEILKNSSYLHD
                     LGKLGIKDDILLKPGKLNKEEFEIIKQHPLITLKILEPLNLRKEEIEACLYHHERIDG
                     SGYPFGLKDEKIPIYAKIIAVADAYSAMTSERPYRNKMSKEEAIEELRRCAGIYFDRD
                     IVELFIEIINQKEEVI"
     gene            1498..1758
                     /locus_tag="PLF90_07025"
     CDS             1498..1758
                     /locus_tag="PLF90_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57065.1"
                     /translation="MKEIEIGKVSHYYTKIGVAAIKVVNDELKKGDTIHIKGHTTDLI
                     QTVESMQKEHEIIDMAKTGDDIGIKVNAHVREGDIVYKIIED"
     gene            1774..2799
                     /locus_tag="PLF90_07030"
     CDS             1774..2799
                     /locus_tag="PLF90_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOK57066.1"
                     /translation="MVRFLNFFSSDLGIDLGTANTCVYVRGKGVVLMEPSIVAINTNT
                     KQIVAVGEEAKKMLGKTPSNIVAMKPMKDGVIADFEACEEMLRYFILKVKTPKHRFVL
                     PPRIVISVPSGITSVERRAVRETALKAGARMALLVEEPMAAAIGVGLPVEEPCGSFIL
                     DIGGGTTEMAVISLGGIVLTKSLRVAGNEMEEAIIEYLKKKYNLLIGERTAEEIKIKI
                     GSAYPLEKELSMDVHGRSLVEGLPKVVRINSEEIREALRDTLNIIVNAVRDILEETPP
                     ELSSDLIERGLVVAGGGALLKGIDKLISQETKLPVYIPEDPLTAVVRGTGKMLEEEKY
                     FKQIPQE"
     gene            2803..3471
                     /gene="mreC"
                     /locus_tag="PLF90_07035"
     CDS             2803..3471
                     /gene="mreC"
                     /locus_tag="PLF90_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOK57067.1"
                     /translation="MLWRFRREVIFTLMMFFSFFIGKTSLKSNISNSDIKIDKEKLLN
                     YDEIIQENRRLREFLSLKEKKILSNFKVAEVIALKPYIFPAEIIIDKGKKDGIAENNL
                     VLTRDIFLIGRVVEVNNSYSRVISIFNKKTKISAIVSSTKEIGVLEGGYAPFLLMKYI
                     PYDSKVKIGDEIYTSGFSEYYHSGIKIGKIVKISKDTNSLFLKIFVKPYISSYDFKEV
                     IICE"
     gene            3464..3943
                     /locus_tag="PLF90_07040"
     CDS             3464..3943
                     /locus_tag="PLF90_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57068.1"
                     /translation="MNRKLWVILEQVLIVSIFQYIFFNFFHLKVIPSFFLVYLLLLTF
                     EGNVEIALFLSFILGVINDIFSKEILGLTSVRYLLVIYFSSFFVVKSIRGKCSLIFVF
                     SFLYFCMLSFRKLDESFWDGKTLLKYAVLFSLYNLLVAIFIEILLREIKKKWTERIF"
     gene            3922..5649
                     /gene="mrdA"
                     /locus_tag="PLF90_07045"
     CDS             3922..5649
                     /gene="mrdA"
                     /locus_tag="PLF90_07045"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOK57069.1"
                     /translation="MDRENFLKNFILFFIYVILLRCFYLQIVRYPYYYQLSIKNCIRT
                     IETGIPRGIIYDRNGRILAKDIPSTNLVFVPYDIKNVEKEIEILSNIVNLDKDYLRKK
                     LTKKYSNPFDRVILKRNLTSMEISLIEENSYNLPGIFVQEGIKRLYTFGKKTSHLLGY
                     LGEISQSQLEKLKEKGYEMGDMIGQDGIEKQYDEYLRGIPGGIQVEVDALGHQRRIFG
                     KKEGQPGNDIILTIDATIQEIASEELGGRQGCVAVMDPRSGEILALVSNPSFDPDNLL
                     YYLDERKHPEKPFLNRVIKGQYPPGSIFKIITEVAALEIGTIKEFDRIECTGVMEVND
                     RIFHCWKEEGHGWVDINLALPFSCNIFFGTVGMKLGVSKLIEYAKKFELGKPTGIDLP
                     GEASGYIPSSQEADPLNLSIGQGPITTTPIQLLSLISTVANGGNIWQPFLGKKIVSPK
                     GEILKEFIPNIKKTVYIDSETLDILRKGLKNVVAFGTGGRANVEGLEIAGKTGTAQRA
                     STEVGLPTHGAFVCYAPFDNPKIAVVVFIDYGSSPHAAEIAGKILKRIFLPEKIEEKD
                     ETETQIFEE"
     gene            5618..6709
                     /gene="rodA"
                     /locus_tag="PLF90_07050"
     CDS             5618..6709
                     /gene="rodA"
                     /locus_tag="PLF90_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOK57070.1"
                     /translation="MKQKLKYLRNSIIEKDIFFLSYFLIFIGLIILFSASKGVILTKE
                     GFFIKQIIWVILGIFLVRIFRNSDYRNLEKSIYFFYLLTIILLFIVLLKGEGRASRWI
                     GLGWFYFQPSEFAKLVIILTLSSYLSRRDVKRFSVLFTSLLIVGIPFFLVFKQPNLGT
                     AIVFILIFIGITYIAGITRKQLIVLFLTAVFLSPLIWAIMKDYQRERILTFLNPMRDP
                     LGKGYNLLQSKITIGSGGFIGKGFMKGTQTKLAFLPEYHTDFIFCVLAEEFGFLGVII
                     FVLIYYYFLSKILEIIYISKDSFGKLVATGIFVMFAGHFLINLGMTLGIFPIVGIPLP
                     FISYGGSSLLVSILSVIVLSSIEKHSTLF"
     gene            6712..8304
                     /locus_tag="PLF90_07055"
     CDS             6712..8304
                     /locus_tag="PLF90_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOK57071.1"
                     /translation="MIEKVEELLPLVENPARYLGREINTYVKKKYEVKIAIGYPDIYE
                     IGMSSLGLRILYGIANERNDCVCERFFLPWIDMESLMRKEGISLFTLETKTPLNKFDM
                     IGISLSSELNYTNFLNILYLSDIPIFAYERKNEHPLIIVGGNSAFNPLPLVPFVDAFF
                     IGEGEEVFNEIIDIIKENKDNRYKILENLNKIDSIFVPLLKKEKVKKAIVKDLDNSFF
                     PLKYLVPLTEITHDRISIEIMRGCVQGCKFCQAGSCWKPVRIRSYNKILEIAKEVYKN
                     TGYEEISLLSFSAGDHPQIEKIVTKLVEEFKLKNVSISFPSLRIDTFSFQLALKIKEI
                     KKTNLTFAPETSERLRKNLGKKITDEELLNLAIQAKANGYRHIKLYFMIGLPDESEED
                     IDEIIKLITEISKVIFVHISFNTFIPKPHTSYQTERFIKKEEYETKRIYITEKLKRNK
                     YVKFNFHPYETSFIECILSRGDEELSKVIYNTWKKGNKFENWKEFFKFKNWLESFEEE
                     KVSIDKYLGYLKPPFKWDFIKL"
     gene            complement(8306..8377)
                     /locus_tag="PLF90_07060"
     tRNA            complement(8306..8377)
                     /locus_tag="PLF90_07060"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8343..8345),aa:Cys,seq:gca)
BASE COUNT         3157 a          950 c         1504 g         2828 t
ORIGIN      
        1 agaaacaaat tggaatcaaa caaaagcggc tgaaaaatta aaattaacaa ggagagtttt
       61 aagatataaa attcaaaaat atggactttt aaaagatgaa agaaagaata ctgataattg
      121 aagataatag agaaataact gaacttatag tgaatatttt gactcttaat aagtttattg
      181 ttgattatgt tttttctgga aatgaaggaa ttgaaaaaac aaaaaaagaa aattatcacc
      241 ttgttatttt agacctgcaa ttacctgata tagatggatt taaagtttta gaagaaataa
      301 aaaaaaataa tgaaaatcta cctgtgatta ttcttacagg ttttggtaca acagaaaata
      361 ttgttaaagc aattaaaata ggagcagatg attttattga aaaaccattt gatataaatg
      421 attttataga aaaggttaag aaatatataa aattgagtag tttagaaaaa gaacttacag
      481 aatttagatt aatagaatcg attttacaat taaacagaac aatcatttct ctttctgatt
      541 tggatacact tttgaatact gtaattcaaa ttatagataa tttattttct cccgacagaa
      601 tagggattta tctttacaat tcagaaaata aaaattatgt cttaaaaaaa tataaattca
      661 aaaatttaca acaaaaaata aaaattgttt ataaggattc cgaaataaaa gaactattta
      721 gagaaaaagg ttttttaatt gaggagttga gggatataca taatttaaag atactgataa
      781 aaggaaagga aaaagatatt ggtatttttg aaataacttt taaagaagat agaaaagtta
      841 aagaagaaga aataaatttt tttgaagcgt tttctattca gattgggatt ggcatagaaa
      901 acgctctttt attagagatg ttacagaatt cttatattaa tgcaattagt tctttaatta
      961 tgtctttgga agcaaaagat aaatatacaa agggacattc agaagaagtt gtttattatt
     1021 ctttattaat tggtaaaaaa cttggactta aagatgaaga acttgaaatt ttaaaaaatt
     1081 cctcatatct tcatgacctt ggtaaattag gaattaaaga tgatatttta ttaaaacccg
     1141 gaaaattaaa taaagaagaa tttgaaatta ttaaacagca tcctttaata acactgaaaa
     1201 tacttgagcc attgaatctc cgaaaagaag aaatagaagc atgtctttat catcatgaga
     1261 gaatagacgg aagtggttat ccttttggat taaaagatga aaaaattcct atatatgcta
     1321 aaataattgc agtggcagat gcttatagtg ccatgacttc ggaaagacct tatagaaata
     1381 agatgagtaa agaagaggca atagaagagt taagaagatg tgcaggtata tattttgaca
     1441 gagatattgt ggaacttttt atagagatta ttaatcaaaa agaggaggtg atataaaatg
     1501 aaggagattg aaattggaaa agtatcccat tattatacaa aaattggtgt tgctgctata
     1561 aaagttgtga atgatgaatt gaaaaaaggg gatacaattc atattaaagg acataccaca
     1621 gatttaattc aaacagtgga atcaatgcaa aaagaacacg aaataataga tatggcaaaa
     1681 acaggtgatg atataggcat taaagtaaat gctcatgtta gagaagggga tatagtttac
     1741 aaaattattg aggattagaa agaaaggaga aaaatggtga gatttttaaa ctttttttca
     1801 agtgaccttg gtatagacct tggtacagca aatacctgtg tttatgtaag agggaaaggg
     1861 gtagttttaa tggaaccatc catagtagca ataaatacga atacaaaaca gattgttgct
     1921 gttggagaag aagcgaaaaa aatgcttggg aaaacacctt caaacattgt ggcaatgaaa
     1981 cccatgaaag acggagttat tgcagatttt gaagcatgtg aagaaatgtt aagatatttt
     2041 attcttaaag ttaaaactcc aaagcataga tttgttttac caccgagaat tgttatttct
     2101 gtaccttctg gaataacaag tgtagaaaga agagcggtaa gggaaacagc gcttaaagca
     2161 ggagcaagga tggctcttct tgttgaagaa ccaatggctg cagcaattgg tgtaggacta
     2221 cctgttgaag aaccttgcgg aagtttcatt cttgatatag gtggtggtac aacagaaatg
     2281 gctgttattt ctctcggagg aatagttctt acaaaaagtt taagagtagc tggaaatgaa
     2341 atggaagaag caattataga atatttgaaa aagaaatata atcttttaat cggtgaaaga
     2401 actgcggaag aaataaagat taaaattggt tcagcttatc cacttgaaaa agaattatca
     2461 atggatgttc atggaagaag tttggttgaa ggattaccta aggttgtaag aattaactct
     2521 gaagaaataa gagaagcgtt aagggatact cttaatataa ttgtaaatgc agtgcgtgat
     2581 atacttgaag agacacctcc ggaactttca agcgatttaa ttgaaagagg tcttgtagtt
     2641 gcaggaggag gagcactttt aaaaggcatt gataaactta tatctcagga aacgaaactc
     2701 cctgtttata taccagaaga tccattaact gctgttgtaa gaggtacagg aaagatgctt
     2761 gaagaagaaa aatattttaa acagattcct caggaataaa aaatgctctg gcgtttcaga
     2821 agagaggtaa tttttacatt gatgatgttt ttttcttttt ttattggaaa gacttcttta
     2881 aaaagtaata tttcaaattc tgatattaaa attgataagg aaaaattgtt aaactatgat
     2941 gaaattattc aagaaaacag aagattgaga gaatttttgt ctcttaaaga aaaaaaaatt
     3001 ttatcaaatt ttaaagtagc agaagtcatt gctttaaaac catatatttt cccagcagaa
     3061 attattatag ataaaggtaa aaaggatggt atagcagaaa ataatcttgt tctaacaagg
     3121 gatattttct taattggaag agtggtggaa gttaataatt catattccag agtaatttct
     3181 atttttaata aaaaaacaaa aataagtgcc atagtttcat ctacaaaaga aattggtgtg
     3241 cttgaaggag gatatgcgcc ttttctatta atgaaatata ttccttatga tagtaaagta
     3301 aaaatcggag atgagattta tacatctgga tttagtgaat actatcattc aggaataaaa
     3361 atagggaaaa tagtaaaaat atcaaaggat actaattcac tttttttaaa aatttttgta
     3421 aagccatata tttcttctta tgattttaag gaggtaatta tttgtgaata gaaaactctg
     3481 ggttatttta gaacaggttt taattgtttc tatttttcag tatatttttt ttaacttctt
     3541 tcatttaaaa gtaatccctt ctttttttct tgtttatctg ttattactta cttttgaagg
     3601 aaatgttgaa attgctttat ttttaagttt tattcttgga gtaattaacg atattttttc
     3661 aaaagaaatt ttaggtttga cttctgttag atatctctta gtaatttatt tttcttcatt
     3721 ttttgtggta aaatcaataa gaggtaaatg tagtttgatt tttgtattca gttttttata
     3781 tttttgtatg ctttctttta gaaaacttga tgaaagtttc tgggatggaa aaactttgtt
     3841 gaagtatgca gttttattct cactttataa tttattagtt gcaattttta ttgaaatttt
     3901 gctgagggaa attaaaaaga aatggacaga gagaattttt taaaaaattt tattttattt
     3961 ttcatttatg taattttatt aagatgtttt tatctacaga ttgttaggta tccatactat
     4021 tatcaactct ctataaagaa ttgtataaga accattgaaa caggaatacc gagaggcatt
     4081 atttatgaca gaaatggaag aatacttgcc aaggatatac cttctaccaa tcttgttttt
     4141 gttccctatg atataaaaaa tgtggaaaag gaaattgaaa ttctttcaaa tatagtaaat
     4201 cttgataaag attatttaag gaaaaaattg actaaaaaat attcaaatcc atttgataga
     4261 gttatactta aaagaaattt aacaagtatg gagatttctt taattgaaga aaactcatat
     4321 aatttaccag ggatttttgt tcaggaaggg ataaaaagat tatatacttt tggaaaaaaa
     4381 accagtcatt tactcggtta tcttggagag atttctcaat ctcaacttga aaaattaaaa
     4441 gaaaaaggtt atgaaatggg tgatatgatt gggcaggatg ggatagaaaa acaatatgat
     4501 gaatacttaa gaggtattcc tggaggaatt caagtagaag tagatgctct gggtcaccag
     4561 agaagaattt ttgggaagaa agagggtcaa ccgggaaacg atataattct cacgattgat
     4621 gcaactatac aggaaatagc ttcagaagaa cttggaggaa gacagggatg tgtagcggtt
     4681 atggatccaa gaagtggtga aatacttgca cttgtgagta atccatcttt tgaccctgat
     4741 aatttacttt attatcttga tgaaagaaaa catcctgaga aacctttttt aaatagagtt
     4801 ataaaaggac agtatcctcc agggtcaatt ttcaaaataa taacagaagt tgctgctctt
     4861 gaaataggta caataaaaga atttgataga atagagtgta caggtgttat ggaagttaat
     4921 gatagaattt ttcattgctg gaaggaagaa ggtcatggat gggttgatat aaatttagca
     4981 ttgccatttt cctgtaatat tttctttgga actgttggaa tgaaattggg agtttccaaa
     5041 ctcattgaat atgcaaagaa atttgaactc ggaaaaccaa caggaattga tttacctgga
     5101 gaagcaagtg gttatatccc ttcttcacag gaagcagacc cattaaatct ttccatagga
     5161 cagggaccaa ttactactac accaattcaa cttttatctt taatttccac cgttgctaac
     5221 ggaggaaata tatggcagcc atttttaggt aaaaaaatag tttctccaaa aggagaaata
     5281 ttaaaagaat ttattcctaa cataaaaaaa acagtatata ttgattcaga aacacttgat
     5341 atactcagaa aaggtttgaa aaatgttgtt gcttttggta cgggaggaag agcaaatgtt
     5401 gaaggccttg aaattgcagg aaaaacaggt acagcacaaa gagcatctac ggaagttggg
     5461 ttacctacgc acggtgcttt tgtttgttat gctccatttg acaatccaaa aattgctgtt
     5521 gtagttttta ttgattatgg ttcttctcca cacgcagcag aaatagcagg taaaatattg
     5581 aagaggatat ttttacctga aaaaatagag gaaaaagatg aaacagaaac tcaaatattt
     5641 gaggaatagt ataattgaaa aggatatatt ttttttgagt tattttttga tatttattgg
     5701 tcttattatt cttttcagcg catcaaaagg tgttattctt acaaaagaag gattttttat
     5761 taaacaaatt atttgggtaa ttctggggat ttttttggtc aggatattta gaaatagtga
     5821 ttacaggaat cttgaaaaaa gtatatattt cttttatctt ttgaccatta ttttactttt
     5881 tattgttttg ctgaaaggag aggggagggc ttcaagatgg ataggacttg gttggtttta
     5941 ttttcaacct tcagaatttg caaaactggt tattatactt actttatctt cttatttatc
     6001 cagaagagat gtcaaaagat tttcagtttt atttacttct ttacttattg ttggtatacc
     6061 ttttttcctt gttttcaaac aaccaaatct tggaaccgct attgtattta tattgatttt
     6121 tattggtata acttatatag caggaataac aagaaaacaa ttaatagtac tttttctaac
     6181 cgcagttttt ctttcccctt taatatgggc tattatgaaa gattatcaga gagaaagaat
     6241 tttaactttt ttaaatccta tgagagatcc tttgggtaaa ggatataatc tacttcaatc
     6301 aaaaattact ataggttcag gtggatttat cggtaagggt tttatgaaag gcacacagac
     6361 aaaactcgct tttcttccag aatatcacac cgattttata ttttgtgttc ttgcagaaga
     6421 atttggtttt cttggagtaa taatttttgt tttaatatac tactattttc ttagtaagat
     6481 tctggagatt atctatatat caaaagattc ttttggaaaa cttgtggcaa cagggatttt
     6541 tgttatgttt gcaggacatt ttttaataaa tcttggtatg acgcttggaa tttttccaat
     6601 agttggaata cctttacctt ttataagtta tggagggtct tctttattgg tttctatttt
     6661 atcagttatt gttttatcaa gtattgaaaa acattcaacc ttattttaaa aatgatagag
     6721 aaagttgagg aattacttcc attagttgaa aatccagcaa gatatcttgg aagagaaata
     6781 aatacgtatg tcaaaaaaaa gtatgaagtc aaaattgcta ttgggtatcc tgatatttat
     6841 gaaataggga tgtcatctct tggtttaaga attctttatg gtattgctaa tgagagaaat
     6901 gactgtgtat gtgaaaggtt ttttcttcca tggattgata tggaaagttt aatgagaaaa
     6961 gaaggtattt ctctttttac tcttgaaaca aaaacccctc tcaataaatt tgatatgatt
     7021 ggaataagtt tatcatctga actgaattat accaattttt tgaacatcct ttatctttct
     7081 gatataccaa tttttgcata tgaaagaaaa aatgaacatc cattaattat tgtaggagga
     7141 aattcagctt tcaatccatt gccactcgtt ccttttgtag atgcattttt tattggtgaa
     7201 ggagaagagg tttttaatga gattattgac ataataaaag aaaataagga taatagatat
     7261 aaaatccttg aaaatctaaa taaaattgat tcaatttttg ttccattatt aaaaaaagaa
     7321 aaagtgaaaa aagcgattgt aaaagatttg gataattcct tctttccttt gaagtattta
     7381 gttccactaa ctgaaataac tcatgataga atttcaattg aaataatgag aggatgtgtt
     7441 cagggttgta agttctgtca ggcgggcagt tgctggaaac ctgtacgaat tagaagttat
     7501 aataaaattc tggaaattgc aaaagaagta tataagaata ctggctatga agaaatttct
     7561 cttttatctt tttcagcagg agaccatcca cagatagaaa agatagtaac aaaacttgta
     7621 gaggaattta aattaaaaaa tgtttcaatt tcatttccat cattaagaat agataccttt
     7681 tcttttcaac ttgcattgaa gattaaagaa ataaaaaaaa ctaatctgac atttgctcct
     7741 gaaacaagcg aacgtttaag aaaaaatttg ggtaaaaaaa ttactgatga agaattacta
     7801 aatcttgcaa ttcaggcaaa agcaaatggt tacagacata taaaattata ttttatgatt
     7861 ggtttacccg atgaatcaga agaggatata gatgagatta ttaaacttat aacggaaatt
     7921 tcaaaggtaa tatttgtaca catttctttc aatacattta ttcctaaacc tcacacttca
     7981 tatcagacag aaagatttat aaaaaaagaa gaatatgaga caaaaaggat atatataaca
     8041 gaaaaactca aaaggaataa atatgtaaag tttaattttc acccttatga aacaagtttt
     8101 atagaatgta ttttaagtcg gggagatgaa gaactgtcaa aagtaattta taatacatgg
     8161 aaaaaaggaa ataaatttga aaactggaaa gagtttttca aatttaaaaa ctggttggaa
     8221 agttttgaag aagaaaaggt ttcaatagac aaatatcttg gatatttaaa accaccattt
     8281 aaatgggatt ttattaaatt atgaaaggcc tgggccggat ttgaaccggc gaatagcggt
     8341 tttgcagacc gctgccttcc cgcttggcta ccaggccatt ttttgattat acctatatag
     8401 ccattttttc tacaagattt aaatactcaa ctcttatag
//
LOCUS       DAOSAT010000076         8392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_5534_length_8392_cov_4.978170, whole genome shotgun sequence.
ACCESSION   DAOSAT010000076 DAOSAT010000000
VERSION     DAOSAT010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5534_length_8392_cov_4.978170"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PLF90_07065"
     CDS             complement(<1..930)
                     /locus_tag="PLF90_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOK57072.1"
                     /translation="MEIEKEELKNLEKVGFDLEEKERAGSFIQKDNEILFSKVFQEGV
                     EVLPIGKAIEKYPEIKEKYYGVSFKKTGKEFEKDTEGGYFIRVKKGVKTLFPVQACLY
                     LKSKKFRQKVHNIIVIEEDASAYLITGCTSAKEAEDAYHLGISEFFISKGGFLNFTMI
                     HSWKEDIEVEPLSAAVVEEGGIFVSNYISLKPVKKIVMYPTALLEGENSRARVSSIIV
                     SFKNSYQDIGSRCILANKNTSAEIISRAVSYGGKIVARGHIKAVSPMTRGHLECRGLI
                     ITEEGIIHAIPELETLYQDVELSHEAAIGKIKKE"
     gene            complement(921..1631)
                     /locus_tag="PLF90_07070"
     CDS             complement(921..1631)
                     /locus_tag="PLF90_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK57073.1"
                     /translation="MLKIENISVKGGDSLIIKDISLFLNKNERLVIFGPNGSGKSTLI
                     KSIMGISGYKVIKGRIIFEDKDITDIPISQRAKMGIGIMYQTPPKIYGIKILQLGKYL
                     AQNEEEINQLAEKLKVDEFLRRDLNVDLSGGEIKRVELFQVLLQKPKLLLLDEPESGV
                     DIENISIMGNVLNEYIKKTECSALIITHTGYILDYVEGEKGCVMMDGKITCQSKPRIV
                     FDMIKKYGYEKCEECKWK"
     gene            complement(1634..3328)
                     /locus_tag="PLF90_07075"
     CDS             complement(1634..3328)
                     /locus_tag="PLF90_07075"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOK57074.1"
                     /translation="MLSDLEIAQRAKIKHIKEIAEKAGIEEEYLELYGNYKAKVSLEI
                     LKKLEGRKNGKYIDVTAITPTPLGEGKTVTTIGLSQAIGKLGKKVFTCIRQPSLGPVF
                     GIKGGAAGGGYSQVIPMEDFNLHLTGDVHAVGIAHNLLAAFVDNSVYKGNPLNIDPLS
                     ITWPRVVDVSDRFLRNIIIGLGGKENGFPRETGFDITVASEVMAILALTTDIFDLRKR
                     LSKIIVAQTYDKKPVTAEDVKAAGAMTVLLKDTIKPTLMQTLEHTPVFVHAGPFANIA
                     HGNSSIIADLIAIKLAEFVVTESGFGADCGMEKFMNIKCRYSGLKPDCVVIVCSIRAL
                     KAHSGKFKVVPGKPLDKGLVEENITALVEGASNLKKQIENAKIFGIPCVVAVNKFITD
                     TEKEIEKVKELAIEFGADDVGVSEVWEKGGEGGIELAEKVIKASEKEKNFQFLYPLDI
                     PIKKKIETIATKIYGAEKVEYSLLAEEKIKLYTKWGFENLPICMAKTHLSLSHDPNLK
                     GAPKGFTLPIRDIRISAGAGFLYPLCGEMRTMPGLPSEPAGTKVDIDEEGKIVGLF"
     gene            3561..4640
                     /gene="mnmA"
                     /locus_tag="PLF90_07080"
     CDS             3561..4640
                     /gene="mnmA"
                     /locus_tag="PLF90_07080"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOK57075.1"
                     /translation="MEKIKVIVGLSGGVDSSVSALLLKERGYEVYGVFMKIFDEKNKD
                     FLGSKSACYGPEEKDIEDVKKICKLLDIPLYVVDLKKEYSEIVLKYFVEEYLEGKTPN
                     PCVICNRFLKFDILLKKAFSLGINFDYFSTGHYARVEYDEKRKKYILKKGIDEEKDQS
                     YFLFLLTQKQLERIIFPLGYYTKKQVREIAKKNNFHLSDKEESQDFLCGDRFFLFSDK
                     KNEGLIVDKDGNILGKHKGIFNYTIGQRKGLGIAKGKPLYVIGINEKENKIIVGEEKD
                     LYKKKLISERVNFISIDKLDKEKEAEAKIRYKHIPAKCIIKPVEENKVEVIFEKGQRA
                     PTPGQAVVFYENDEVIGGGFISKTF"
     gene            4693..5661
                     /locus_tag="PLF90_07085"
     CDS             4693..5661
                     /locus_tag="PLF90_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOK57076.1"
                     /translation="MGLWFIRVFFLILCVLTGFYSLPNQKILGVLIGFLGSIFVIGIE
                     IMIGKIPWKKLILAVGGLIVGLLTAILLANFFLLIPFEDQKMENLIRFSLYFVFSYLG
                     IMAGIKGFEELGFILPFFGGIKEGEKFLVVDTSILIDGRVYELMKSGFIDYTLIIPQF
                     IIKELQGLADASSDIKRQRGRRGLEILNKLREDGEINVKVYNVDYPDIEAVDTKLVKL
                     ASELNAAILTNDFNLTKVAEVQNIKVLNFNTLTNLMRPRLMSGEEISLKIIKEGKEAG
                     QGVGYLEDGTMVVVENASKYIGKVVDIVIDSAIQTPTGRIIFAKLK"
     gene            5658..6329
                     /gene="ispD"
                     /locus_tag="PLF90_07090"
     CDS             5658..6329
                     /gene="ispD"
                     /locus_tag="PLF90_07090"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOK57077.1"
                     /translation="MIFTILVGAGKGKRLNYSIPKSMIEIGGKKLFLYSIEKFYKFSD
                     KILLAVPHGYIKKCRDMTKNIFPDLIVVKGGKRRQDSVLNCLNYIDEKEGIVLIHDIA
                     RPFVSEELIKKVIEKTKRYGACIPVLKITDTVKEVEKKFVKRTLNREKLFAVQTPQGF
                     RLGLIKRAYKRGEKLKGSDDSFFLEKIGYNKIYCEEGVRTNIKITYREDLFFAEFLLK
                     KWEKE"
     gene            6314..7501
                     /gene="argJ"
                     /locus_tag="PLF90_07095"
     CDS             6314..7501
                     /gene="argJ"
                     /locus_tag="PLF90_07095"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOK57078.1"
                     /translation="MGKGITYPEGFYASGINCGIKEGKNDLSLIYSESPCISSGTFTT
                     NQFKSYSVLWCIKNIKNETRAILANSGNANACNGKENWDLTKKIMEELAKKLKIEKNS
                     VLFASTGMIGVPLPYEKIKNGLDKLIQNLSKDGNLEATKAIMTTDTFPKEYEIETGIE
                     GRKKQVVIGGMAKGAGMINPSMATMLCFITTDAVIDRKALDFALKEAVEDSFNMITVD
                     NDTSTNDTVICLANGLARNKTIHIGSPEFNSFSNSLKKLCIELAKKIALDGEGASKFI
                     EVTVNGGWCKKDAKRVAKRVCGSSLVKTAVSGGDPNWGRIVAAVGSTNARMDRKNIEI
                     KICGIKVFEGEPVKFDRELLRELMTKKEIKIEIDLKRGNFSATSWGCDLTEEYVRINR
                     EYS"
     gene            7498..>8392
                     /locus_tag="PLF90_07100"
     CDS             7498..>8392
                     /locus_tag="PLF90_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57079.1"
                     /translation="MISKVKKFTIFFLGDESKLLERLQKFGMVEIENISFEGFEKKTL
                     SKDEIEDKIRKLEFLKNILIESGEKEINEKIIIDEKEEKEIVENFPLENIYKKHFQLY
                     QEIEKRKKIIQRIEKLKEEIMPFLDTEIIFSEIFSLTSFSFFVFSLSQKTKFENTFED
                     LYITKIGEFQKDTLYIVLFRKDKREVAENFLKEIAGKILYVRNWNKKIKDIFEKLEKV
                     KEKNEKVISEIKSEISKIKINRKEIFVCYDYYKSIYEFVIAKEKLGISKFVKGIKGWI
                     SEKNIDTLKEIVLQILPESHIV"
BASE COUNT         3028 a         1171 c         1341 g         2852 t
ORIGIN      
        1 ttcttttttt attttaccta ttgctgcttc atgagaaagt tcaacatcct gataaagtgt
       61 ttcaagttcg ggtatggcat gtattattcc ttcttctgtt attattaatc ccctgcattc
      121 aagatgtcct cttgtcattg gtgaaactgc ttttatatgt cctctcgcaa caattttacc
      181 tccataactt accgctctgc ttataatttc tgcagatgta tttttatttg caaggataca
      241 tctgcttcct atatcctgat atgaattttt aaatgaaact ataattgaag aaacccttgc
      301 tcttgaattt tctccctcaa gtaaagcagt tggatacatc acaatttttt taacaggttt
      361 taatgatata taattggaaa cgaaaatacc accttcttct actacagcag cacttaatgg
      421 ctctacttca atatcctcct tccatgaatg aatcattgta aaatttaaaa aaccaccttt
      481 tgaaataaaa aactctgata ttccgagatg atatgcatct tctgcttctt ttgcagaagt
      541 acatcctgta attaaatacg cagaagcatc ctcttcaata acaattatgt tatgaacttt
      601 ttgtctgaat ttttttgatt ttaaatataa acatgcctga actggaaata atgtctttac
      661 accctttttc acccttataa aatacccgcc ctctgtatcc ttttcaaatt ctttacctgt
      721 ttttttaaaa gaaactccat aatatttttc ttttatttca ggatatttct caattgcttt
      781 tccaattggt aatacttcta ctccttcctg aaaaaccttt gaaaaaagaa tttcattatc
      841 tttctgaata aaactacccg ctctttcctt ttcttctaaa tcaaacccaa ctttttcaag
      901 attttttaat tcctcttttt ctatttccat ttacattcct cacatttttc atatccatat
      961 ttttttatca tatcaaaaac tattctgggt ttactctgac aggttatctt tccatccatc
     1021 ataacacatc ctttttctcc ctcaacataa tcaagtatgt atcctgtatg agtaataatt
     1081 aaagcagaac attctgtttt ttttatatat tcattcaata catttcccat aattgaaata
     1141 ttttcaatat caactcctga ttctggttca tcaagtaata acaatttcgg tttctgcaat
     1201 aaaacctgaa ataattcaac ccttttaatt tctccccctg aaaggtcaac atttaaatcc
     1261 cttcttaaaa actcatcaac ctttaacttt tcagcaagtt ggtttatttc ttcttcattt
     1321 tgagcaagat attttccaag ttgtaaaatt tttattccat aaattttagg gggggtttga
     1381 tacattatac ctatacccat tttagccctc tggctaatag gaatatctgt aatatcctta
     1441 tcttcaaaaa taatcctccc ttttatcact ttataaccag aaattcccat tattgatttt
     1501 ataagagttg atttacctga accatttggt ccaaaaatca ccaatctctc atttttatta
     1561 agaaaaagag aaatatcctt tattattaaa ctgtctccac cttttacact tatattttct
     1621 atttttaaca tttttaaaat aaccctacaa ttttaccctc ttcatctata tctacttttg
     1681 ttcctgcagg ttcacttgga agtcctggca tggttctcat ttctccacat aaaggatata
     1741 aaaatccagc accagcagaa attcttatat ctcttatggg taaagtaaat ccttttggag
     1801 caccttttaa attcgggtca tgtgataaag aaagatgtgt ttttgccata cagattggaa
     1861 gattttcaaa tccccatttt gtatataact taattttttc ttctgcaagt aatgaatatt
     1921 caactttttc tgctccataa atctttgttg caattgtttc aatttttttc tttatcggta
     1981 tatcaagcgg gtataaaaac tggaaattct tttctttttc acttgcttta ataactttct
     2041 cagcaagttc aattccacct tctccacctt tttcccagac ctcgcttact ccaacatcat
     2101 ccgctccaaa ttcaattgca agttctttta ctttttctat ttctttttct gtatctgtaa
     2161 tgaatttatt tacagcaaca acacaaggaa ttccaaaaat tttagcattt tctatctgct
     2221 tttttaaatt actcgctcct tcaacaagtg cagtgatatt ttcttcaaca agtcctttat
     2281 caagcggttt tcctggaaca actttgaatt taccactgtg tgcttttaat gctcttatag
     2341 aacaaacaat aacaacacag tcaggtttaa gaccgctata tctgcatttt atattcataa
     2401 acttttccat accacaatca gcaccgaatc cactttctgt aacaacaaat tcagcaagtt
     2461 taatagcaat aaggtcagca attattgaac tattaccatg agcaatattg gcaaaaggac
     2521 ctgcatgtac aaaaacaggt gtatgctcaa gtgtctgcat aagagttggt tttatagtat
     2581 ctttaagtaa aacagtcatt gctcctgctg cttttacatc ctctgctgta acaggttttt
     2641 tatcgtatgt ctgggcaact ataatttttg agagtcgttt cctaaggtca aagatatcag
     2701 ttgtaagagc aagtatcgcc attacttctg aagcaactgt tatatcaaaa ccagtttctc
     2761 ttggaaaacc attttcttta cccccaagtc caataataat atttcttaaa aatctatcag
     2821 aaacatcaac aactctgggc caggttatag aaagtgggtc aatatttaaa ggatttcctt
     2881 tatagacaga attatcaaca aaagcagcaa gtaaattatg agcgatacca acagcatgaa
     2941 catctcctgt cagatgtaaa ttaaaatctt ccattggtat aacctgtgaa tatcctccac
     3001 ctgcagcacc acctttaatg ccaaaaacag gaccaagaga aggttgtctt atacaggtaa
     3061 aaaccttctt accgagtttt cctattgcct gagaaagtcc aattgttgta actgtttttc
     3121 cttctccaag aggtgttggt gttattgctg taacatcaat atatttaccg ttttttcttc
     3181 cttcaagttt tttcaggatt tcaagagaaa cttttgcttt atagtttcca tacaattcaa
     3241 gatattcttc ttcaattcct gctttttcag caatttcttt aatatgttta atttttgccc
     3301 tctgtgcaat ttcaaggtca cttaacattt ctacctcctt gttaatttat atagtttaat
     3361 ttttagaata atctttgtca aacttttgaa ttaagacaaa tcccttccaa tcccacctta
     3421 gaggtgggaa atggagatga ttgatagaca aaggttttgt gaaggagatt gtgttattct
     3481 tcaatttttc tcttgttttc atatagttta aatctttaaa ataaactgta tcaaatactt
     3541 atagatggta aaataatatt atggaaaaaa taaaagtgat tgttggactt tctggaggag
     3601 ttgattcttc tgtaagtgct ttacttttaa aagaaagagg ttatgaggta tatggtgtgt
     3661 ttatgaaaat ttttgatgag aaaaataaag attttttagg aagtaagagt gcctgttatg
     3721 gtcctgaaga aaaagatatt gaagatgtaa aaaaaatctg taaactcctt gatatacctt
     3781 tatatgtcgt tgatttaaaa aaggaataca gtgaaattgt tttgaaatac tttgtagagg
     3841 aatatcttga aggtaaaaca cccaatccct gtgttatatg taatagattt ttaaagtttg
     3901 atattttatt gaaaaaggca ttttctctcg gtataaattt tgattatttt tcaacaggtc
     3961 attatgcaag agtagagtat gatgaaaaaa gaaaaaaata tattcttaaa aagggtattg
     4021 atgaagaaaa agaccagagt tattttttat ttttacttac acaaaaacaa ctggaaagaa
     4081 ttatttttcc tcttggttat tatactaaaa aacaggtaag ggaaatagca aaaaagaata
     4141 attttcattt atcagataaa gaagaaagtc aggatttttt atgtggagac aggttttttc
     4201 tttttagcga taaaaaaaac gaaggattga tagttgataa ggatggtaat attcttggaa
     4261 aacataaggg tatttttaat tatacaatag gacagagaaa gggacttgga attgcaaaag
     4321 gaaaaccatt atatgtaatc gggataaatg aaaaagaaaa taaaattatt gttggagaag
     4381 aaaaggactt atataaaaaa aaattaattt cagaaagagt taactttatt tctattgata
     4441 aacttgataa agaaaaagaa gcagaggcaa aaataagata caaacatatt cctgcaaaat
     4501 gtattataaa accagtagaa gagaataaag ttgaggttat ttttgaaaaa ggacaaaggg
     4561 caccaactcc cggacaggca gttgtttttt atgaaaatga tgaagttatt ggtggtggtt
     4621 ttatatctaa aactttttaa attttacaaa ttttgacttt tatgctataa aaaacaaagg
     4681 aggtgaatta aaatgggttt atggtttata agagtttttt tcttaatttt atgtgtttta
     4741 acaggttttt acagtttacc aaatcagaaa atacttggtg ttttaatagg atttttgggt
     4801 tctatctttg taataggcat tgaaataatg atagggaaaa ttccatggaa aaaactcatt
     4861 cttgcagttg gtggtttaat agtggggctt ttaacagcaa ttcttcttgc aaacttcttt
     4921 cttttaatcc cttttgaaga ccagaaaatg gaaaatttaa taagattttc cttatatttt
     4981 gttttttcat atcttgggat tatggcagga ataaaaggat ttgaagaact tggttttatt
     5041 ttaccttttt ttggtggaat aaaagaagga gaaaaatttt tagtcgttga tacaagtatt
     5101 ttgattgatg gaagggttta tgaacttatg aaaagcgggt ttattgatta tacacttata
     5161 attccccagt tcataattaa agaattacag ggacttgcag atgcttcaag tgatattaaa
     5221 agacagagag gaagaagagg acttgagatt ttaaataaat taagagaaga tggagaaata
     5281 aatgtaaaag tttataatgt tgattatcct gacatagaag cagtggatac aaaacttgta
     5341 aaacttgctt ctgaacttaa tgctgcaatt ctaacgaatg attttaatct tacaaaagta
     5401 gcagaagtcc agaatataaa agtccttaat tttaacactc tgacaaattt aatgagacca
     5461 aggttgatga gtggagaaga aatttcttta aaaattataa aagaagggaa agaagcaggt
     5521 cagggagtcg gataccttga agatggaact atggttgtgg tagaaaatgc ctcaaaatat
     5581 attgggaaag ttgttgatat agttatagat agtgctattc agacaccaac aggaaggatt
     5641 attttcgcaa aattgaaatg atttttacta ttctggtagg tgctggtaaa ggaaaaagat
     5701 taaattattc aattccgaaa tcaatgatag aaattggagg taaaaaatta tttctttatt
     5761 ctattgaaaa attttataaa ttctcggata aaatattgct tgccgtaccg catgggtata
     5821 ttaaaaaatg cagagatatg acaaaaaata tattccctga tttaatagtc gtaaagggtg
     5881 gcaaaagaag acaggatagc gttttaaatt gtttaaatta tattgatgaa aaagaaggta
     5941 tagttttaat tcatgatatt gcaagaccat ttgtgagtga ggaattgata aaaaaagtta
     6001 ttgaaaaaac aaaaagatat ggtgcatgta ttcctgtttt aaaaataacg gatactgtaa
     6061 aagaagtaga aaaaaaattt gttaaaagga ctcttaacag agagaaactt tttgctgtcc
     6121 agacaccgca ggggttcaga ttaggattga taaaaagagc atataaaagg ggtgaaaaat
     6181 taaaaggaag tgatgactct ttttttcttg agaaaatagg gtataataaa atctattgtg
     6241 aagaaggagt aagaacaaat ataaaaataa cttatagaga agatttattt tttgcagaat
     6301 ttttattaaa aaaatgggaa aaggaataac ttatcctgag ggtttttatg caagtggaat
     6361 taactgtgga ataaaagaag ggaaaaatga tttatcttta atttattcag aatcaccttg
     6421 tatttcttca ggtaccttta caacaaatca atttaaatca tacagtgtac tctggtgtat
     6481 aaaaaatata aaaaatgaaa caagggctat tctggcaaat agtggaaacg caaatgcctg
     6541 taatggaaaa gaaaactggg atttaacaaa aaaaataatg gaagaacttg ctaaaaaact
     6601 aaaaattgaa aaaaattcag tcctttttgc ttcaacaggg atgataggag ttcctctacc
     6661 atatgaaaaa attaaaaatg gacttgataa actaattcaa aacctgtcaa aagatgggaa
     6721 tttagaagca accaaagcga taatgacaac ggatacattt ccaaaagaat atgaaataga
     6781 aacaggaata gaagggagaa aaaaacaggt tgtaatcgga ggaatggcaa aaggagcagg
     6841 tatgataaat ccttcaatgg ctacaatgct ctgcttcatt acaacagatg ctgttataga
     6901 tagaaaagca cttgattttg ctttaaaaga agcggttgaa gattcattca atatgattac
     6961 agttgataat gacacgagta caaatgacac tgttatctgt cttgcaaatg gtcttgcaag
     7021 aaataaaaca attcatattg gaagtcctga atttaattct ttttcaaatt cactgaaaaa
     7081 attatgtatt gaacttgcaa aaaagattgc ccttgatgga gaaggagcaa gtaagtttat
     7141 tgaagtaact gtcaatggtg gatggtgtaa aaaagatgca aagagagtgg caaaaagagt
     7201 atgtggttct tctcttgtaa aaacagcagt aagcggtgga gaccctaact ggggaagaat
     7261 agttgctgca gttggttcaa caaatgcaag aatggataga aaaaatatag aaattaaaat
     7321 atgtggtatt aaggtttttg agggagaacc ggtaaaattt gacagggaat tactgagaga
     7381 attaatgaca aaaaaggaaa taaaaattga aattgattta aagagaggta atttttctgc
     7441 aacatcatgg ggatgtgatt taacagaaga atatgtgaga attaacaggg aatattcatg
     7501 ataagtaaag ttaaaaaatt cactatcttt ttccttggag atgagagtaa actgctggaa
     7561 agattgcaga aatttggtat ggttgagatt gaaaatattt cttttgaggg ctttgagaaa
     7621 aaaacattat caaaagatga gatagaagat aaaataagaa aacttgaatt tctgaaaaat
     7681 atacttattg aatcaggaga aaaagaaata aatgaaaaaa taattataga tgaaaaagaa
     7741 gaaaaagaaa ttgttgaaaa ttttcccctt gaaaatattt ataaaaaaca tttccaactt
     7801 tatcaggaaa tagaaaaaag aaaaaaaatt atccagagaa tagaaaaatt aaaagaagaa
     7861 attatgcctt ttcttgatac tgagataatt ttttcagaaa ttttttctct taccagtttt
     7921 tctttttttg ttttttctct ttcacaaaaa acaaaatttg aaaacacttt tgaagattta
     7981 tatataacaa aaatagggga gtttcagaaa gacactttat acattgtttt attcagaaag
     8041 gacaaaagag aagttgctga aaatttttta aaagaaattg ccggaaaaat tttatatgtt
     8101 agaaactgga acaaaaaaat aaaagatatt tttgaaaaac ttgaaaaagt taaagaaaaa
     8161 aacgaaaaag tcatttcaga aataaagagt gaaatttcaa aaataaagat aaatagaaag
     8221 gaaatttttg tatgttatga ttactacaaa agtatttatg aatttgttat tgccaaagaa
     8281 aaacttggca tttcaaaatt tgtaaaaggg ataaagggtt ggataagtga aaaaaatata
     8341 gatactctga aggagatagt tttacagatt ttacctgaaa gtcatatagt ta
//
LOCUS       DAOSAT010000077         8326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_5580_length_8326_cov_4.006045, whole genome shotgun sequence.
ACCESSION   DAOSAT010000077 DAOSAT010000000
VERSION     DAOSAT010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5580_length_8326_cov_4.006045"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..1096
                     /locus_tag="PLF90_07105"
     CDS             356..1096
                     /locus_tag="PLF90_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOK57080.1"
                     /translation="MLRVLKPEIKGNAYDYSIYKKEKVNFLIPLEVIYRNYLPEGSSV
                     FRRLKERTLKLEDIGLIEVPKVGQKLEKPIIDISTKLELTDRYINWKEAKEIANLSDD
                     EIEEIKIKTLKINEIITNEVEKIGLRNEDGKVEFGFDENRNLILVDAVGTLDECRFTY
                     NGIPVSKEIARIYYRKTEWYKKVEEAKKKNKIEWKKYVDIEPPNLPEELFKTISDIYR
                     SYTNEITEKKWFDVPPLKEILSKIKKYI"
     gene            1098..2066
                     /locus_tag="PLF90_07110"
     CDS             1098..2066
                     /locus_tag="PLF90_07110"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOK57081.1"
                     /translation="MVEVLKPSEEAIEKAINILKNGGVIAFPTETVYGLGANGLNSDA
                     VIKIFEIKKRPFFDPIILHIGKKEDLEKLWKKINKKAEKLIDKFWPGPLTIILPKTEV
                     VPYIVTAGLETVAVRMPSHPVALEILKRVDFPIAAPSANIFGRLSPTEAKHVKQQIGN
                     RIELIIDGGRCSVGIESTVIDLTGERSLILRPGGVPKEEIEKVIGKVEIIKKTEKISS
                     PGQLPFHYSPKTPLKILYDYNEVKKGIKAGLLAFKKPKNKDKFVRIEVLSMKGDLREA
                     AVNFFSCLHKLDEAKLDIIYAEPVPEQGLGTAIMDRLHKAETKNFI"
     gene            complement(2075..3613)
                     /locus_tag="PLF90_07115"
     CDS             complement(2075..3613)
                     /locus_tag="PLF90_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57082.1"
                     /translation="MNNFYPIPQPDLSYTITSAIGIGVMLLICTLLGFLSRKKSVTFE
                     TFLSGHQDIGPVITGLALGATWLSGWATLGMMGVTYAVGWSGMWFAGIWTIVGIMPCL
                     FFTGEKMQKYAEKFGAKTVPDVLRIRFDSKIVQFLSALIMIFFMTLYAVGQFKAGATI
                     WYAVTGLPALWCLLLSGIIVFIYMIVGGYTGTQWSLALQGALLGIACFVLGIVSIFFV
                     GGPSELNAKLSTQDIKLLDLIRRDLPAVGNTQLFSSPVGIIATFFIFLTMATGFPHNV
                     ARFLGMKKLTKKDFSLITLMVFLVAGPPIFLNAITGLISRATFGPEILNIQPWKGDLA
                     APYLAIVAGGKPLTVLYTTGVFAAALSTLSAMVMVMAGNVTKDIIYTFKPNISKNLLL
                     KLTKVFIGIFLLIPFYWTFKKPPALLAVFMGYASIGLGSIFIFCTAVSFYWKRATSTG
                     AILCMLYGVIATLIGSIFVFKQKLGMGTLEFIVLIGCGIFYFVGSLLTKPLSEEKLKQ
                     LFEK"
     gene            complement(3821..6832)
                     /locus_tag="PLF90_07120"
     CDS             complement(3821..6832)
                     /locus_tag="PLF90_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOK57083.1"
                     /translation="MDLLEKWEESVKNLEQRINKYLHLNEWKFKQNCSEGAKVEIDDS
                     NWEKKTQPIHWALKDGDTYLRKWIEIPEEIEGIKIEGSKIYLKFVFPSGVTLFINGKE
                     VYSHKFWADKIATPFLLTENAKPNEKFLIVFKTDKGDGLGGFWAYLSIKNLNDIVFQL
                     KSILYQLKFAQIILKETKKKKLSEIIKKCYEKLDSDLIIKKDWEKIKNVIKDIENLLE
                     SFKPYAKKFKVHLIGHAHIDMNWLWTYEDTVSVVLRDFTTIINLMDKYKDLTFSQSQT
                     HVYDITKENDKKLFKKVKDKVKSGNWEITANAWVENDLNMSDGESIVRHIIYSKKYIK
                     ENFDKDVKIMWSPDTFGHPFTIPSIIKNADINYYYFMRCGKGYPLFIWEGPDKNKVIA
                     FNSIYNNQIDSDRIIPLFIDYYKRYKIKDFMFVYGVGDHGGGPTEEDIERKIKLNEKP
                     CFPNLEFSTTEKYFKIIEKYKNRLPIVKDELNFIFEGCYTTHSDIKRNNRNCENILLK
                     LETLSSIFSINNKQYPENEIEKMWQKTLFNQFHDILDGSAIHKSYDYSNTLANEVIEE
                     GNKIINEIIEKMKIENKSITIFNPNGWETKYPAKIPYYGNEDIHIEDECGVKIDAEKT
                     EGNLIFTTTPISPYNFKTFYIKDCKIYRSGFIKREEEWENEFYYIWVDKKTGLIRGIY
                     DKKNKKDVIPKANSIPEDKSSFWAENCANLIKLYWEKPHPMSAWIIGNIYKIENLIDL
                     DNLEIKEGNTLVIFEVIRKYKETKIIQRTLLYRDFPFIDFEFETDWNIKGNSEIGVPM
                     LRANFNFNAQRSDFYCEIPFGAMKRKNIPREYPALRWAGFKEDNWWAVIMNREKYGYF
                     LDGNNLSLTLLRNSYEPDAEPDCGHHLVSYRLFFGKSNISEITKMALEYNITPVISEG
                     EIKKQKLFYIEGNIVPTCFKKSIKDNTYILRMVEYEGKKGKFVITFDRNIKNLWISNV
                     KEENLKRIPVKNKKIELNFKPHEIITLKIEF"
     gene            complement(6918..>8326)
                     /locus_tag="PLF90_07125"
     CDS             complement(6918..>8326)
                     /locus_tag="PLF90_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK57084.1"
                     /translation="AIDLIDEAASKLKMEIDSMPVELDEIERKLTQLEIEKQALKKEN
                     TPEVKERLEKLEKEIVEIKKERDTLYKQWITEKEIVEKIRKIKEQIELARNIIEKAER
                     EGDLDKAAEYKYGKLIELNKKLEEETKKLREVQKEKGLLKDEVTEEDIAEIVSKWTGI
                     PVQKMLEGEVEKLIHMEERLSKRVIGQEEAIKAVSNAIRRNRAGLSDPNRPIGSFIFL
                     GPTGVGKTELAKALAEFLFNNENALVRIDMSEYMEKHTVSRLIGAPPGYVGYEEGGQL
                     TERIRRQPYSVILLDEIEKAHPDVFNILLQLLDDGRLTDGHGRTVDFRNTVIIMTSNI
                     GSQFITSFKEEDVIKEKIMETLKLKFRPEFLNRIDEIIIFKKLKKEDILKIVGIQISY
                     LQKRLSEKKIKIEITEKTKNYLAEKGYDENFGARPLKRLIQREIENPLALKIISGEFK
                     EGDTIIIDVENGALKFTK"
BASE COUNT         2798 a         1325 c         1128 g         3075 t
ORIGIN      
        1 tacgtacctg tggaaggata tctgacaatg gtggaaaccg ccagaaaata ccgcggcgaa
       61 taataataca ggtatcagtc aaactctacc acaatggttt cgaacagttc cagcttggga
      121 agggcaaagg ataattcgtt cctgccgcca atactgtagt ccaaaactat acctccacac
      181 agcgctttta cgccggcaac ccgtctgcct accttcaggc gaacggatat atcaaatatg
      241 ggtaacggct caaaaaaatg gaataaaaac acattataac ggaattgtag ataaaaatgg
      301 ttatgttaga agtttaggag aaataaaaga gccaacaaac ataattgaat ttaaaatgtt
      361 aagagtttta aaaccagaaa ttaaaggaaa tgcttatgac tattcaattt ataaaaagga
      421 aaaagtgaat tttctaatac cccttgaagt tatatacaga aattatttac cggagggttc
      481 atctgtattt agaaggttaa aagaaaggac tttaaaattg gaagatattg gtttgataga
      541 agtaccaaag gtaggtcaaa aacttgaaaa accgataatt gatatttcaa caaaacttga
      601 attgactgac agatatataa actggaagga ggcaaaagag attgcaaatt taagtgatga
      661 tgaaattgaa gaaataaaaa taaaaacatt aaaaataaac gaaataataa caaatgaagt
      721 agaaaaaatt ggacttagga atgaagatgg taaggttgaa tttggttttg atgaaaatag
      781 aaatttaatt cttgttgatg ctgttggaac tcttgacgaa tgcagattta cttataatgg
      841 aataccggtg agtaaagaaa ttgcaagaat atattataga aaaactgagt ggtataaaaa
      901 ggttgaggaa gcaaagaaga aaaataaaat tgaatggaaa aaatatgtgg atatagaacc
      961 tccaaatttg ccagaagaac tatttaaaac aatatctgat atttacagaa gttatacaaa
     1021 tgaaataaca gaaaagaaat ggtttgatgt tcctccttta aaagaaatac tttcaaaaat
     1081 taaaaagtat atttgaaatg gtagaggttc ttaaaccttc tgaagaagca atagaaaaag
     1141 caattaacat tttaaaaaat ggtggagtta ttgcatttcc aactgaaact gtttacggtc
     1201 ttggggcaaa tggtttaaat tcagatgcag taattaaaat atttgaaatt aaaaaaagac
     1261 ctttttttga ccctataatt ttacatattg gaaaaaaaga agatttagaa aaactctgga
     1321 aaaaaataaa taaaaaagca gaaaaactta ttgataaatt ttggcccggg cctttaacta
     1381 taatattgcc aaaaactgaa gttgttcctt atatagttac tgctggactt gaaacagttg
     1441 ctgtaagaat gccatctcat cccgttgcac ttgaaatttt aaaaagagtt gattttccaa
     1501 tagcagcacc aagtgcaaat atttttggaa gattgagtcc aacagaagca aaacatgtaa
     1561 aacaacaaat agggaataga attgaactta ttatagatgg tggtagatgt tctgttggta
     1621 ttgaatcaac agtgattgat ttaacagggg aaagaagttt aattttgaga cccggagggg
     1681 ttccaaaaga agagattgaa aaggttattg gaaaagttga gattataaag aaaacagaaa
     1741 aaatttcttc tccgggacaa ttaccttttc attattcacc caaaactcct cttaaaattt
     1801 tgtatgacta taatgaagta aaaaaaggga ttaaagcagg gcttcttgca tttaaaaaac
     1861 caaaaaataa agataaattt gtgagaattg aggtgttatc tatgaagggt gatttaagag
     1921 aagcagcagt gaattttttt tcctgtcttc ataaacttga cgaagcaaaa cttgatataa
     1981 tttatgcaga accagttccc gagcagggac ttggaacagc aataatggat agattacata
     2041 aagcagaaac aaaaaatttt atttagattt taaattactt ttcaaaaagt tgttttaatt
     2101 tttcctcaga taatggtttt gtcaataaac ttcccacaaa atagaaaatt ccacatccaa
     2161 taagaactat gaattcaagt gttcccattc caagtttttg tttaaaaaca aatatacttc
     2221 caataagagt ggcgatgact ccataaagca tacatagaat tgcaccggtt gatgttgctc
     2281 ttttccagta aaaagaaact gctgtacaga atataaaaat actaccaagg cctattgagg
     2341 catatcccat aaaaaccgca agtagagctg gaggtttttt aaatgtccag taaaaaggaa
     2401 ttaaaaggaa tatacctata aaaactttag ttaatttgag aagtaaattt ttagaaatat
     2461 taggtttaaa ggtgtaaatt atatcttttg ttacatttcc agccataacc attaccatag
     2521 cagaaagtgt agaaagtgct gctgcaaaaa caccggttgt atataaaaca gttaatggtt
     2581 taccaccagc aactatagca agatatggtg cagcaaggtc tcctttccat ggctgtatgt
     2641 ttaaaatttc aggaccaaaa gttgctcttg aaataagtcc tgtaattgca tttaaaaata
     2701 ttggtggacc tgcaacaaga aataccatga gagtgattaa agaaaaatct ttttttgtta
     2761 gttttttcat tccaagaaat cttgcaacat tatgaggaaa acctgttgcc atagttaaaa
     2821 atataaaaaa agttgcaatt atcccaacag gagaagaaaa taattgagta tttccaactg
     2881 caggaagatc tcttcttatc aaatccagaa gttttatgtc ttgagtagaa agttttgcat
     2941 ttagttcaga tggtcctcct acaaaaaaaa tagaaacaat acccaataca aaacaggcaa
     3001 ttccaagtaa tgctccctgc aaagcaagag accactgagt tcctgtatat ccaccaacaa
     3061 tcatataaat aaaaacaata atcccagaaa gaagaagaca ccataaagca ggaagtcctg
     3121 ttactgcata ccaaatggta gcacctgctt taaattgacc tactgcataa agagtcataa
     3181 agaatatcat aataagagca gatagaaatt ggacgatttt gctatcaaat cttattctta
     3241 gtacatctgg gactgttttt gctccgaatt tttcagcata tttctgcatt ttttcgcctg
     3301 taaaaaataa acaaggcata attccaacta ttgtccatat cccggcaaac cacattcctg
     3361 accaaccaac tgcatatgta acgcccatca ttccaagtgt tgcccatcca cttaaccatg
     3421 ttgcaccaag tgcaagtccg gtaataacag gtcctatatc ttgatgtcct gataaaaagg
     3481 tttcaaaagt tactgatttt tttctgctta aaaatccaag tagtgtgcag attaataaca
     3541 ttaccccaat tccaattgct gatgtaatag tataagatag atctggttgc ggaataggat
     3601 agaagttatt catttttcca ccccctttat tcttatcaat aatcctatta ttgtgaaaaa
     3661 tgtactaaaa aatataacta accaccatgc caaccagttt ttgtccatat cccctccttt
     3721 ttgaaaaaaa tattaaacta ttatataaaa atgtcaaatt tttatttttt gatttgaaat
     3781 gaaaataaac aaaatgatag ggaaatttta ttttatattt ttaaaactct attttaagag
     3841 taattatttc atgcggttta aaattaagtt caattttttt attttttact ggtattcttt
     3901 ttaaattttc ttcctttaca ttactaatcc agagattttt tatatttctg tcaaaagtta
     3961 taacaaattt tccttttttt ccttcatatt caaccatcct taaaatatat gtattatctt
     4021 taattgattt tttgaaacaa gttggtacaa tatttccttc aatatagaat aatttttgtt
     4081 tttttatttc tccttccgaa ataacaggtg taatattata ttcaagagcc atttttgtta
     4141 tttctgaaat atttgatttt ccaaaaaata atctatatga aactaaatga tgtccacaat
     4201 caggttcagc gtctggttca taagaatttc ttaacagggt taaagataag ttatttccat
     4261 ccaggaaata tccatatttt tctctattca ttattactgc ccaccagtta tcttctttaa
     4321 atcctgccca tcttaaagct ggatattccc gaggaatatt ttttcttttc atagcaccaa
     4381 aaggtatttc acaataaaaa tcacttcttt gagcattgaa attaaagttt gcccttaaca
     4441 taggtacccc aatttcacta tttcccttta tattccaatc tgtttcgaat tcaaaatcaa
     4501 tgaaaggaaa atctctgtaa agtaaagttc tttgaataat ttttgtttct ttatattttc
     4561 ttataacttc aaaaataact aatgtgtttc cttcctttat ttcaagatta tccaaatcta
     4621 ttaaattttc tatcttataa atattaccta ttatccaggc actcatagga tgtggttttt
     4681 cccaataaag ttttattaga ttagcacaat tttctgccca gaatgaactt ttatcttctg
     4741 gaattgaatt tgcttttggt attacatctt ttttattttt tttatcataa atccctctta
     4801 taaggcctgt ttttttatct acccagatat aataaaattc attttcccat tcttcctctc
     4861 tttttataaa accacttcta taaattttac aatctttgat ataaaatgtt ttaaagttat
     4921 aaggggaaat aggagtagtt gtgaatataa gatttccttc agttttttca gcatctattt
     4981 ttacaccaca ctcatcttca atatgtatat cttcatttcc ataatatggt atttttgcag
     5041 gatattttgt ttcccagcca tttggattaa aaattgttat acttttattt tctatcttca
     5101 ttttttcaat tatttcattt attattttgt ttccttcttc aataacttcg ttagcaagag
     5161 tattggaata atcgtatgat ttatgtattg ctgaaccatc aagtatatca tgaaattgat
     5221 taaaaagtgt tttttgccac attttttcaa tttcattttc cggatactgt ttgttattaa
     5281 ttgagaagat tgaagaaaga gtttcaagtt ttaacaatat attttcacaa tttctattat
     5341 ttctttttat atcagaatgt gttgtataac aaccttcaaa aataaaattt aattcatctt
     5401 taacaatggg taatctattt ttatattttt ctattatctt gaaatatttt tcagtagttg
     5461 aaaattcaag atttgggaaa catggttttt cattaagttt tatttttctc tcaatgtctt
     5521 cttctgtagg tcctcctcca tggtctccaa ctccataaac aaacatgaaa tcttttattt
     5581 tgtatctctt ataataatca atgaagagtg gaataattct atcgctatca atttgattat
     5641 tgtaaatact gttaaaagca attactttat ttttatcggg accctcccat ataaataatg
     5701 gatatccctt tccacatctc ataaaataat aatagtttat atctgcattt tttataattg
     5761 agggtatggt aaatggatgg ccgaatgtat caggagacca cataattttt acatctttat
     5821 caaaattctc ttttatatac ttttttgaat aaattatatg tctcacaata ctttctccat
     5881 cagacatatt taaatcattt tcaacccatg catttgctgt tatttcccag ttaccacttt
     5941 ttactttatc ttttactttt ttaaaaagtt tcttatcatt ttctttagta atatcataaa
     6001 catgtgtttg actttgggaa aaagttaagt ccttatattt atccatcaag tttataattg
     6061 ttgtaaaatc tcttagaact acagaaacag tatcttcata tgtccataac cagttcatgt
     6121 caatatgagc atgacctata agatgaacct taaatttttt agcatagggt ttaaaacttt
     6181 ccagaaggtt ttcaatatct tttataacat tttttatttt ttcccaatct tttttaataa
     6241 taaggtctga atcaagtttt tcataacact ttttgattat ttcagaaagt tttttctttt
     6301 ttgtttcttt taaaattatt tgagcaaatt ttaattgata tagaattgac tttaattgaa
     6361 aaacaatatc atttaaattt ttgattgata aatatgccca gaaaccccca agtccatcac
     6421 ccttgtctgt tttgaaaact ataaggaatt tttcgtttgg tttagcattt tctgttaata
     6481 aaaatggagt tgcaatttta tctgcccaga atttatgaga ataaacttct tttccattta
     6541 taaacagtgt tacacccgaa ggaaaaacaa atttcaaata aatcttactt ccttctattt
     6601 ttattccttc aatctcttcc ggtatttcta tccattttct taaataagta tctccatcct
     6661 ttaaagccca atgaattggt tgagtttttt tctcccagtt actatcatct atttcaactt
     6721 ttgctccttc tgaacaattt tgtttaaatt tccattcatt aagatgtaag tacttattaa
     6781 ttctctgttc aagatttttt acactttctt cccatttctc caataaatcc atgtttgcct
     6841 cctttaaaag ttttctaaca agtatatatt ttaaattaca aaattagaaa atcaagttta
     6901 tttttgatgg ggaaaaatta ttttgtaaat tttaaagcgc cattttcaac atctataatt
     6961 attgtgtctc cttctttaaa ttctccagaa ataattttaa gggcaagggg attttctatt
     7021 tctctctgaa ttaaacgttt taaaggtctg gctccaaaat tttcgtcata acccttttct
     7081 gcaagataat tttttgtttt ttctgtaatt tcaattttaa ttttcttttc agagagtcgt
     7141 ttctgaaggt atgaaatttg aatacccaca attttgagaa tatcttcttt ttttaatttt
     7201 ttaaatatta ttatttcgtc aattctgttt aaaaattccg gtctgaattt taattttaaa
     7261 gtttccatta ttttttcttt tattacatct tcttctttaa atgaagtaat aaattgactt
     7321 ccaatgtttg atgtcataat aatcacagta tttctgaaat ctacagttct tccatgtcca
     7381 tccgtcagac gtccatcatc aagtaattga aggagtatat taaaaacatc aggatgtgct
     7441 ttttctattt catccagaag aatcacacta taaggttgtc ttcttattct ttctgttaat
     7501 tgccctcctt cttcataacc tacatatcca ggaggagccc ctattaaacg agacactgta
     7561 tgtttttcca tatattcact catatcaata cgaacaagag cattttcatt gttaaataga
     7621 aattcagcaa gtgcttttgc aagttctgtt tttcctactc ctgtaggtcc gagaaagata
     7681 aaagaaccaa ttggtctatt tggatctgag agacctgctc tatttcttct tatagcattt
     7741 gaaactgctt ttattgcttc ctcctgtccg ataactcttt ttgataacct ttcttccata
     7801 tgaattaatt tttcaacttc tccttcaagc attttttgaa caggtattcc tgtccatttt
     7861 gaaacaattt ctgcaatatc ttcttccgta acttcgtctt ttaataatcc cttttctttc
     7921 tggacttctc ttagtttttt cgtttcctct tcaagttttt tatttaattc aatcaattta
     7981 ccatatttat attctgctgc tttatcaagg tctccttctc tttccgcctt ttcaattata
     8041 tttcttgcaa gttcaatttg ttctttaatt tttcttattt tttcaactat ttccttttct
     8101 gtaatccact gtttgtataa tgtatctctt tcctttttta tttcaactat ctctttttct
     8161 aatttttcta atctttcctt gacttcaggt gtattttctt tttttaatgc ctgtttttct
     8221 atttcaagtt gcgttaattt tctttcaatt tcatccagtt caacaggcat actatcaatt
     8281 tccattttaa gtttgcttgc agcctcatct atcaaatcta ttgctt
//
LOCUS       DAOSAT010000078         8287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_5613_length_8287_cov_4.134961, whole genome shotgun sequence.
ACCESSION   DAOSAT010000078 DAOSAT010000000
VERSION     DAOSAT010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5613_length_8287_cov_4.134961"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PLF90_07130"
     CDS             complement(<1..906)
                     /locus_tag="PLF90_07130"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF024927.2"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOK57085.1"
                     /translation="MTEENCGVVGVYSNKNCSEKIFYSIFKLQHRGQKYCGISTFNKK
                     INLITHEGFVRHSFTQSELKNLSGNYGIGHVSLKEKQPILLESRLGTFSIAFAGNIIN
                     FDDLFKEEKNKGHSFFSHTQIELIAKIIVQSNDFVEGLEKLSQKVRGSYTILILCKEG
                     IYAARDPYGFKPLILGKNNEDYIISSESRPFAFLKAQIVRNVKPGEILLINENGIQTK
                     KIIKGKRIAFCAFEWGYIASMDSIIEGVSVAKARNNLGYFLAKNDDVKADIVSAIPFS
                     GIGYALGYHKGSKIPYDEVFLIDRFA"
     gene            complement(903..1616)
                     /locus_tag="PLF90_07135"
     CDS             complement(903..1616)
                     /locus_tag="PLF90_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HOK57086.1"
                     /translation="MKGQDIQSELEAIYRISQAITSDMYLEDILKLIVNVTAEIFKSK
                     ICSILLYDEKEKVLKIRATQTMSNEYLKKPPLKVGEGIAGKVFETKKPIVVEDVRKEK
                     EYKYRDIAKKEGLVSMLSIPMVVKNRSIGVLNVYTTYPYKFTQKEIEIISSIANQAAI
                     VIENTELLVKTKILEEELESRKKIEKAKGILMKEEKLTEEEAYDKIRKFSMNKRISMK
                     EVADAIILAHEIKKEENKK"
     gene            complement(1674..2828)
                     /locus_tag="PLF90_07140"
     CDS             complement(1674..2828)
                     /locus_tag="PLF90_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57087.1"
                     /translation="MRNEEIINRLSDFDKEVREKSLHEIFDLIKRKRIEKDNEFEGYL
                     NAHIHTFHSFNYKNWSPSRIVFEAWKKGLLYLGTVDFDTLAGLEETLYAGKLLNIKVI
                     SGFESRVFLKEMKDKVINSPNEPGIFYLCGKGFRKIPEEGTEEKKFFDNLKKIAQDRN
                     KKVIEKVNNYLKDVKIDYEKDVLPLTPSNNPTERHIILAYYLKSKENLGEKTDIFWAN
                     ILGIEKEKIIELKGKNIGDLYEKIRQKLIKFGGPGYVKSESSDFPLFDDVVKMTERAG
                     GIPIGTYLDGTNEGEKDPEFLVELLRNKGIKGITIIPERNWNIKDEREKKIKVKNLEQ
                     FMNVCKKMNMPVICGTEMNKYGQPFVDNFKSPEIYRYLDYFIKSFEKLFL"
     gene            2919..3245
                     /gene="trxA"
                     /locus_tag="PLF90_07145"
     CDS             2919..3245
                     /gene="trxA"
                     /locus_tag="PLF90_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOK57088.1"
                     /translation="MEEIVLHLNKDNFNEVIKNNEKVVVDFWASWCMPCRMVAPFFEN
                     LAKKHKGEIVFAKVNVDENPEIAAKFGVMSIPDFIIFKNGEKKGEIVGAMPEEILEEK
                     ILNYFK"
     gene            complement(3248..3802)
                     /gene="gmk"
                     /locus_tag="PLF90_07150"
     CDS             complement(3248..3802)
                     /gene="gmk"
                     /locus_tag="PLF90_07150"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOK57089.1"
                     /translation="MKHLIFVLSAPSGTGKTTIQKILKKELKDIDTVITYTTRKPRPN
                     EKNGIDYNFVDKEKFEEMIKRDEFAEWSVVYGNYYGTPKKKIEENLKKNKKTLLVIDT
                     QGGLKIKKKYPEVFLIGILPPSLKEQERRMRERKDMSDEEIKKRLLISKEERKILFKY
                     YDVRLINKNIEKTVKRIKKIILES"
     gene            complement(3803..4732)
                     /locus_tag="PLF90_07155"
     CDS             complement(3803..4732)
                     /locus_tag="PLF90_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57090.1"
                     /translation="MKKNWIRIFIAILLLIPIGFLQVSIDYSKQVENLESNILLMPGQ
                     MAGSLILSGFRGLAADLLWLNIENYWHSGQHYKMLPLLDAIAWLQPTYIVVWAVGGWH
                     MTYNIFANVRSRANTLDEELGKILSKLEDKSIKETITQIRKDIKDLVEGLAREYPKTE
                     EKIKEYFDITNNCYKKIDALKEKKEYKELYEIVSGLINVPVEMLFWYTNGISFLKKGI
                     TYNKDRYDLYFELGWTYYHKGMDYPNAVRYLEKAVKFPHPEFVDDVLAHAYEKNGQID
                     MAIKQWEKLIGTSFESIAQRAIHSLKTTGKFTP"
     gene            complement(4729..5493)
                     /locus_tag="PLF90_07160"
     CDS             complement(4729..5493)
                     /locus_tag="PLF90_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOK57091.1"
                     /translation="MKKAWIIGLNSFKESLRKKTLYILLVVALIVIGASKAFSFLTAE
                     EELKMIKDVSFSSIEFFGALIAIFMSLTAISSEIEKRTIYTLFSKPITRQNFFIGKFL
                     GIILIIFLNFILMCLFFTGLLIFKKSPPDIQVFKTLLLIFIELILIGSITLTVATFAS
                     DAFNVIFSFFLYIVGHLTSYGNQLADRTENIILKGLWKILYTVIPNYENFNIRDKVVV
                     GIDVSWNYILKTGVYGILYLAVVLLIGSYFIQKREI"
     gene            complement(5490..6245)
                     /locus_tag="PLF90_07165"
     CDS             complement(5490..6245)
                     /locus_tag="PLF90_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK57092.1"
                     /translation="MNVIEIKELTKYYFLGRNKVVVGLENVSFNVEEGEIYGILGPNG
                     SGKTTCLKLMLGILYPTSGEITILGKNQFDVEMKKNVGFLPENPYYYDYLTGPELLRF
                     YGKLFEIPEKVLNERIEKLLFLVKLHENKKLALRHYSKGMLERIGLAASLINDPKILI
                     LDEPTTGLDPIGCIETRDLLIQLKREGKTILLSSHFLSEVERVCDRIAIFHRGYLLAT
                     GKLNELIQKYNATGLEDLFVKTIEEFDKKGENQ"
     gene            complement(6284..7081)
                     /locus_tag="PLF90_07170"
     CDS             complement(6284..7081)
                     /locus_tag="PLF90_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOK57093.1"
                     /translation="MEEIIKNWKKEIDEYLDKKLPTKKDCPSVLSEAMRYTVFSGGKR
                     LRPILVLCVSKILGIDKKKVLPVACGIELIHNFSLVHDDLPSMDNDDFRRGVPTCHKK
                     FGEGIAILVGDALLTYGFQLIAESGKISLVKDVAEAIGYKGMAGGQILDLIYKGKEIK
                     DEEKRKIDYMKTGRLFEICFKVPFYFKKVEKEKKEKIENIAKNFGIAFQIRDDIEDKE
                     GDEIILKEKLKKIYQKLKQETLFFGKKGKILLYIFDRIYFDFLNNNL"
     gene            complement(7065..8174)
                     /gene="alr"
                     /locus_tag="PLF90_07175"
     CDS             complement(7065..8174)
                     /gene="alr"
                     /locus_tag="PLF90_07175"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOK57094.1"
                     /translation="MEKRAWIEVDISKIRKNILKIKQITGKKFLASVKCNLYGMGVKE
                     IAKEIDDIVDFYGVACFSEAIELKNLKVKKPVLIMGNVLPENIEECILNDIRITLCNE
                     ENLEEIIKICKKYQKNAFIHIKIDTGMGRIGLRNDEIIPFIQKCLDFKGIKIEGIFSH
                     FATAEWKDKTYANYQLNIFKKEIKKIENFIKVPIKHIANSGAILNLPESYKEFDMVRI
                     GLLILGIYPAKYLAKKIKFDCAIKGFTRIVFIKQVEKNTPLSYGLSYTTKRKTKVATC
                     CIGYGDGLRRFLSNNFYLKWKGEKVKIIGNICMDQTLLDVTGKNVKIGDKIEIFGDNY
                     EIETMAEIGNTVPQEILCGFGSQRIEKIYKNGRNN"
BASE COUNT         2581 a         1399 c          915 g         3392 t
ORIGIN      
        1 tgcaaatcta tcaatcaaaa aaacttcatc ataaggaatt ttagaccctt tatgataacc
       61 aagagcatag ccaattccag aaaatggaat tgctgaaaca atgtctgctt tcacatcatc
      121 attttttgca agaaaatatc cgagattatt tcttgctttt gcaacagaaa caccctcaat
      181 tattgaatcc atggaagcaa tataacccca ttcaaatgca cagaaagcaa ttctttttcc
      241 ttttataatt ttttttgttt gaataccatt ttcatttatt agaagaattt ctccaggttt
      301 aacatttctt acaatttgag cttttagaaa agcaaatgga cgcgattccg aagaaattat
      361 ataatcttca ttattcttgc caaggattaa aggtttaaac ccgtaagggt cccttgcagc
      421 ataaattccc tctttacaga gaattaaaat tgtatatgaa ccccttactt tttgagatag
      481 tttttcaagt ccctcaacaa aatcattact ttggacgatt atttttgcaa taagttctat
      541 ctgtgtatga ctaaaaaatg aatgtccttt atttttctct tctttgaata aatcatcaaa
      601 attaattatg tttcccgcaa aagcaataga aaaagttccc aatcttgatt caagtagaat
      661 tggctgtttt tcttttaaac tcacatgccc tattccgtaa ttaccggaaa gattttttaa
      721 ttcagattgt gtgaaggaat gtctaacaaa gccctcatgg gttattaagt taattttttt
      781 gttaaaagta gatatcccgc aatatttttg tccacgatgt tgtaacttaa aaatactata
      841 aaaaattttt tcactgcaat ttttgtttga gtaaactcct accacaccgc agttttcttc
      901 tgtcattttt tgttttcttc cttttttatt tcatgagcaa gaattatggc atcagcaact
      961 tctttcatac taattctttt attcatgcta aattttctta ttttatcgta tgcttcctct
     1021 tctgtcaatt tttcctcttt cataagaata ccttttgctt tttctatttt ttttcttgat
     1081 tcaagttctt cttcaaggat ttttgttttt actaaaagtt cagtattttc aataacaatg
     1141 gctgcttgat ttgctataga agaaattatt tctatttctt tttgtgtaaa tttataaggg
     1201 tatgtcgtgt acacatttaa aaccccaatt gacctatttt tcacaaccat tggtatagat
     1261 aacattgaaa caagtccttc cttttttgca atatctctat atttatattc tttttctttt
     1321 ctcacatctt caactacaat cggttttttt gtttcaaaca ccttcccagc aattccttct
     1381 cctactttta atggtggttt ttttaaatat tcattactca ttgtttgtgt tgccctaatc
     1441 tttaaaactt tttccttttc atcatacagt aaaattgaac atatttttga tttaaaaatt
     1501 tcagcggtca cattaacaat aagttttaaa atgtcttcaa gatacatatc agacgtgatt
     1561 gcctggctta ttctatatat tgcttctaat tcagattgta tatcttgtcc tttcatattt
     1621 tttattctac catagattat atgaattatc gtaacaatat aaaaatagag aatttataaa
     1681 aataactttt caaaactttt tatgaaataa tcaagatatc tgtaaatttc tggtgattta
     1741 aaattatcaa caaatggttg cccatattta ttcatttctg tcccgcatat aacaggcata
     1801 ttcatttttt tacagacatt cataaattgt tcaagatttt taacttttat tttcttttct
     1861 ctttcatctt ttatattcca gtttctttca ggtattattg ttataccttt tatcccctta
     1921 tttctaagta attctacaag aaactcaggg tctttttctc cttcatttgt tccatcaagg
     1981 tatgtcccaa ttggtatacc tcctgccctc tctgtcattt ttacaacatc gtcaaataaa
     2041 ggaaaatcac tactttcact ttttacataa ccaggacctc caaatttaat aagtttctgc
     2101 cttatttttt catataaatc acctatattt tttcccttta attcaataat tttttccttt
     2161 tcaataccta aaatatttgc ccagaagata tctgtttttt cacctaaatt ttcttttgat
     2221 ttcaagtaat aggcaagaat aatatgcctt tcagttggat tattagaagg tgttaaaggt
     2281 aatacatctt tttcataatc aatttttaca tctttcaagt aattattgac cttttctata
     2341 acctttttat ttctgtcttg agcaattttt tttaaattat caaaaaattt tttttcttca
     2401 gtaccttctt ctggaatttt tctaaatcct ttaccgcata gataaaatat accgggttca
     2461 tttggagaat ttataacttt atctttcatt tcttttagaa atactcttga ttcaaaacca
     2521 ctaattactt ttatattcag tagtttccca gcatacaatg tttcttcaag ccctgcaagt
     2581 gtatcaaaat caactgtacc aagatataat aatccctttt tccatgcttc aaaaacaatt
     2641 cttgaaggcg accagttttt gtaattaaag gaatgaaaag tatgaatatg tgcattcaaa
     2701 taaccctcaa actcattatc tttttctatt ctctttcttt ttatcaaatc aaatatctca
     2761 tgcaaacttt tttctcttac ttctttatca aaatcagata atctatttat aatttcttca
     2821 tttctcatga ttttaatagt atatcatata tttcgtatat ttttaaggtt ttaaattcaa
     2881 tagaattcta tggtaaaata aataaaaagg aggaatttat ggaagaaata gtattacatt
     2941 taaataagga taattttaat gaggtaatta aaaataatga gaaagtagtt gtagatttct
     3001 gggcgagttg gtgtatgcca tgtagaatgg ttgctccttt ttttgaaaat cttgcaaaaa
     3061 agcataaagg agaaattgtt tttgcaaaag ttaatgttga tgaaaatcct gaaattgctg
     3121 caaaattcgg tgttatgtca atccctgatt ttataatctt caaaaatgga gaaaaaaaag
     3181 gagaaattgt aggagcaatg ccagaagaaa ttttagaaga aaaaattttg aattatttta
     3241 agtaatttta tgattccagg attatttttt ttattctttt cacagttttt tctatatttt
     3301 tatttattaa tctcacatca tagtatttaa acaatatttt cctttcttct tttgatatta
     3361 acaatctttt ttttatttct tcatcactca tatcttttct ttctctcatt ctccgttctt
     3421 gttcttttaa agatggaggt aaaattccaa ttaaaaacac ttctggatat ttttttttaa
     3481 tttttaatcc tccttgagta tctataacaa gaagtgtttt tttgtttttt tttaaatttt
     3541 cttcaatttt cttttttggt gttccataat aattaccgta aaccacagac cattctgcaa
     3601 attcgtctct tttaatcatc tcttcaaatt tttctttgtc aacaaagtta tagtcaatgc
     3661 catttttttc attgggtctt ggttttcttg tggtataagt tataactgta tcaatatcct
     3721 ttaattcttt ttttagaatt ttttgtattg tagtttttcc tgttcctgac ggtgctgaaa
     3781 gaacaaatat taagtgtttc atttatggtg taaatttacc tgtagtttta agtgaatgaa
     3841 ttgctctttg agcaatactt tcaaaagaag ttccgattag tttttcccat tgttttattg
     3901 ccatatcaat ttgtccattt ttttcataag catgtgctaa aacatcatca acaaattcgg
     3961 gatgaggaaa tttcactgcc ttttcaagat atctaacagc attgggataa tccatacctt
     4021 tatgataata agtccatccg agttcaaaat aaaggtcata tctatcttta ttataagtga
     4081 ttcccttttt taaaaatgaa attccgttag tataccaaaa tagcatttcg acagggacat
     4141 ttattaaacc ggagacaatt tcatataatt ctttatattc ttttttttct tttaaagcat
     4201 caattttttt ataacagtta tttgtaatat caaaatactc ctttatcttt tcttctgttt
     4261 ttggatattc tcttgcaagt ccttcaacta aatcttttat atcttttctg atttgagtta
     4321 ttgtctcttt tatagatttg tcttccagtt ttgataaaat ctttccaagt tcttcatcca
     4381 gtgtatttgc ccttgaacgt acatttgcaa atatattata tgtcatatgc cagcccccaa
     4441 cagcccagac aactatatat gttggctgaa gccatgcgat tgcatcaagt aacggaagca
     4501 ttttatagtg ttgacctgaa tgccagtagt tttctatatt taaccataaa aggtctgctg
     4561 caagacctct gaatccactc aatattaaac ttccagccat ctgtccgggc atcaatagaa
     4621 tattactttc aagattttcc acttgttttg aataatcaat acttacctgt aaaaagccta
     4681 taggaattaa aagtaatatc gcaataaata ttctaatcca attttttttc atatttctct
     4741 cttttggata aaataggaac caattaaaag aacaactgca aggtataaaa ttccataaac
     4801 ccctgttttc agtatataat tccagctaac gtcaattcca actactactt tatcccttat
     4861 attaaaattt tcataattag gaataactgt ataaagtatt ttccagaggc cttttaaaat
     4921 tatattttct gttctgtcag caagttgatt tccatatgaa gttaaatgtc ccactatata
     4981 aaggaagaag gaaaaaatta cattaaaagc atcagaagca aaagtcgcaa cagtaagagt
     5041 aattgaacca attaaaatga gttcgatgaa aatcagtaat aatgttttaa atacttggat
     5101 atcgggtgga ctttttttga aaattaatag accagtgaaa aataaacaca taaggataaa
     5161 gtttaaaaaa ataattagaa taattcccaa aaatttcccg ataaaaaaat tctgtcttgt
     5221 tattggtttt gaaaataatg tgtaaatcgt tcttttttct atttcacttg aaattgctgt
     5281 aagagacata aaaattgcta ttagagctcc aaaaaattct atgcttgaaa aactgacatc
     5341 tttaatcatt tttaattctt cctctgccgt aaggaaagaa aatgcctttg atgcaccaat
     5401 aacgataagt gctactacta gtaatatgta taaagtcttt ttcctcaacg attctttgaa
     5461 actattcagt ccgataatcc atgctttttt cattgatttt ctcccttttt gtcaaattct
     5521 tctattgttt ttacaaataa atcttcaaga cctgttgcat tatatttctg tattaactca
     5581 ttaagtttcc cagtagcaag cagataacct ctatgaaata tagcaattct atcacatact
     5641 ctttcaactt cggaaagaaa atggcttgaa agtaaaatag tttttccctc tctttttaat
     5701 tggattaata aatctcttgt ttcaatacaa cctatagggt caagacctgt tgttggttca
     5761 tcaagtatta aaatttttgg gtcatttatt aagcttgctg caagcccaat tctttcaagc
     5821 atgcctttgg aataatgtct taaagcaagt tttttgtttt catgaagttt aacaagaaat
     5881 aataattttt caattctttc atttaaaact ttctcgggta tttcaaaaag ttttccataa
     5941 aatcttaaaa gttctggacc tgttagataa tcatagtaat aagggttttc aggtaaaaaa
     6001 ccaacatttt ttttcatttc aacatcaaat tgattttttc caagaatagt tatttctcct
     6061 gaagttggat acaaaattcc cagcataagt tttaaacagg tcgttttacc tgaaccattt
     6121 ggtccaagta tcccataaat ttctccttct tcaacattaa aggatacatt ttcaagaccc
     6181 acaactactt tatttcttcc aagaaaataa tattttgtta actcctttat ttctattaca
     6241 ttcatttgat tcctcctcta aaaaaattta atactatatt atatcacaaa ttattattta
     6301 aaaaatcaaa ataaattcta tcaaatatgt aaagtaagat ttttcctttt tttccaaaaa
     6361 acaaagtttc ttgttttaat ttttgataga tttttttcaa tttttctttt aatattattt
     6421 catctccttc tttatcttct atatcatctc ttatttgaaa agcaattcca aaatttttcg
     6481 ctatattttc aattttttct tttttttctt tttctacttt tttaaaataa aatggtactt
     6541 taaaacatat ttcaaaaagt cttcctgttt tcatataatc aatttttctt ttttcttcgt
     6601 cttttatttc tttcccttta taaattaaat caagaatctg tcctcctgcc attcctttat
     6661 atccaatcgc ttccgcgaca tcttttacta aacttatttt tccactttct gcaataagtt
     6721 gaaatccata ggttaaaagg gcatcaccca ctaaaatagc aataccttct ccaaattttt
     6781 tatgacaggt agggactccc cttctaaaat cgtcgttatc cattgaaggt aaatcatcgt
     6841 gtacaagaga aaaattatgt ataagttcta ttccacacgc aactggtaaa accttttttt
     6901 tatcaattcc caaaatctta cttacacata gaactaaaat tggtcttaat cttttcccac
     6961 ctgaaaaaac agtatatctc attgcttcac ttaaaacact aggacaatct tttttagtgg
     7021 gtaatttctt atcaaggtac tcatctattt cttttttcca gtttttaatt atttcttcca
     7081 tttttgtaaa ttttttcaat tctttgactc ccaaaaccac acagtatttc ctgtggtact
     7141 gtattaccta tttctgccat tgtttcaatt tcgtaattat caccaaatat ttctatttta
     7201 tcacctattt taacattttt tcctgtgaca tctaaaagtg tttgatccat acatatattt
     7261 ccaatgattt taactttttc acctttccat tttaaataga agttattaga aagaaatctt
     7321 ctcagtccgt ctccatatcc aatacaacag gttgcaactt ttgtttttct ttttgtcgta
     7381 tatgaaagtc cataacttaa aggagtattt ttttcaacct gtttaataaa aacaattctt
     7441 gtaaaacctt ttattgcaca atcaaatttt attttttttg ccagatactt agcaggataa
     7501 attcccagta tcaaaagacc tattctaacc atatcaaatt ctttataact ttctggtaag
     7561 tttaaaatag cacctgaatt tgctatgtgt ttaattggaa ctttaataaa attttcaatt
     7621 ttttttattt cttttttgaa aatatttaat tgataatttg cataagtttt atctttccat
     7681 tcagcagtgg caaagtgaga aaatatgccc tctattttta tacctttaaa atcaagacat
     7741 ttttgaataa atggtattat ttcatcattt cttaacccaa ttcttcccat tcctgtgtca
     7801 atttttatat gtataaatgc atttttttga tactttttgc agatttttat tatttcttct
     7861 aaattttctt cattacataa agttattctt atatcattta aaatacattc ttcaatattt
     7921 tcaggtaaaa cgtttcccat tataagaaca ggttttttaa cttttagatt ttttaattca
     7981 atcgcttcac taaaacaagc tactccataa aaatctacaa tatcatctat ttcttttgct
     8041 atttctttta cacccatacc gtaaagatta catttaacac ttgcaagaaa tttttttcct
     8101 gttatttgct ttatctttag aatatttttt cttattttac ttatatccac ttcaatccat
     8161 gctctttttt ccattttcaa tctcttttat tattttttca gcaatttttt caggttgagg
     8221 attttcatat attggtctac caaccactct aaaatctgct ccctttttaa tagcttcttt
     8281 aggagta
//
LOCUS       DAOSAT010000079         7924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_5925_length_7924_cov_4.886898, whole genome shotgun sequence.
ACCESSION   DAOSAT010000079 DAOSAT010000000
VERSION     DAOSAT010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5925_length_7924_cov_4.886898"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLF90_07180"
     CDS             <1..387
                     /locus_tag="PLF90_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOK57095.1"
                     /translation="LGLEVEISKFKKRVIKCPNCGLKYYKPIEKQTDVKIATKIFEIF
                     YKKISEILVIISGDTDLVPAIRTTKETFDDIKIGVVIPYKNSSEELKLVSDFYYSIPP
                     RKYINHLLPNPYKLKTGEVICKPEKW"
     gene            403..1176
                     /locus_tag="PLF90_07185"
     CDS             403..1176
                     /locus_tag="PLF90_07185"
                     /inference="COORDINATES: protein motif:HMM:NF037887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cthe_2314 family HEPN domain-containing protein"
                     /protein_id="HOK57096.1"
                     /translation="MKNLFFEYYKIDEEEWNKISKNSSQKFKKLFWKIPYEKYQVLLD
                     DDIEKVYKKGKINELVKALMNKMGTLRISYIFLTYYYNLGIPDDKWCISPGKNGSSIQ
                     YFPDFEEKHFYIHYFFNFWAEVFYIKFFSIIDIICDLLNNYYEMGIKKETKGFRRKVI
                     KNLKKENLELFKFLENIKNKREYKNAKEFRNRIIHSIPAYEISNQNEIKKYENKIVEI
                     KFGAGKYTKSSEIKENAEEVLILLKNIFEKLKENLNFDD"
     gene            1189..2397
                     /locus_tag="PLF90_07190"
     CDS             1189..2397
                     /locus_tag="PLF90_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOK57097.1"
                     /translation="MNWNFLPILEVCEFEKGTEPGSESYNRDGMGIPFIRVGNIGAKI
                     QEQIYTTSENVKICKKDDILIALDGSPGIVIRGLEGAYSSGIRKVIVKDSTKLMKEFV
                     YYYLQTDEVQKIINEHTIGVTIKHAGKSLNFINIPIPPISVQKKIVKILDTVQKGIEI
                     QEKIIEKTKEIKKSLMADLFKYGAPSFRKNRKSKKTEIGEIPEDWKVVRLGEVCEKIE
                     QKNPANEPLKKIVYIDVSSLDNEYFKIVEHKEYLGKEAPTRARKIIKFGDVIFATVRP
                     YLKRIALVPENYNNAYCSTAFCVIRPIKEKLLSGFIFYFLTLDKFVQNVSTNQHGSGY
                     PAITDNHIYKVKIPLPSLSEQKEIAEILSVIDEKIEIEKKKKELYEELFKSLLNKIMN
                     GEIDVEKIEL"
     gene            2419..5412
                     /locus_tag="PLF90_07195"
     CDS             2419..5412
                     /locus_tag="PLF90_07195"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HOK57098.1"
                     /translation="MEKYEKKLVEDYVSERLKEIGWKFVSYENLKRESIKEPLLVENL
                     KEAIIKINKDKGIGEEEIKKVIDEIKLLSNHQEGIKSFLHYLKYGIGIKFEKERVVKI
                     IDLIDYERPENNEFIFSRQVRFKGSDLIIPDIVLYINGIPIVEIECKSPIDLKTDWLT
                     GYEQIKNYEKIMPELYKYVQIGISFCEKVRYFPIVPWQDKVDVYIWKKDELPEDEAIF
                     EFLNPYILLDILRNFLFIREVHGEIKKVICRYMQYKAVNKIYNRVVDNLKGKETKNKG
                     LVWHWQGSGKTLTMIFATHKLYFEKYLENPTIFIVVDREELEEQMKEELSSLKLNFSF
                     EVIESVRNLKEVITHDNYSGKRGVFLTLIHKFRPDEKFLPDGLPDSIAHRKNVICFLD
                     EVHRTQYGLLKSCMKNILKNAFYFGFTGTPIAENERNTYNEFGYPLEDEGYLDKYFLD
                     QSQKDGFTLPLVYEIRLEHLHIPEEDFKFFVEKWEEDEFKGDIDLHPEISVKVRKKLN
                     HINLFLEKEDRIDEICKDIGKHFKENIDGKFKGMVVCGSRKACVIYKKYLNKYLPPEY
                     SEVVMSFKIDEKEPINSFYYEWKKRYSGFANDKERIKKIIDDFKEKEYPKILIVTDML
                     ITGFDAPILHVLYLDKLLKKHRLLQTIARTNRPYGDVKECGFIIDYAGVIKNLKYALK
                     EYYKSEIKGLITEYDKLFEEFEKCIENMKEIFKNIKFKIEREVLLKSLDILRDDKIRD
                     RFFENYKKTRKIFEILASHPEKLKYLKEFKWFSAVYDYYRKLTEDEEEKELVDKFFKK
                     TVDFVHKNLKVKEIKKTLPSLKLDLKTIEEIRKSAIPEKEKVVNIIFILSKLVYVERG
                     KNPVYRTIADRIDDLIRKWKERKIEIEELFKEEKEILKEIEIKEKEKEKLEFDNFDYG
                     LYLILKDEIKEDEITLKNYVEEIKNGIKDSMVENWIENPALRQVIERKTREICLEIKN
                     KHNLDYEKFDLLHRKIIEFVKEYGS"
     gene            5402..5929
                     /locus_tag="PLF90_07200"
     CDS             5402..5929
                     /locus_tag="PLF90_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOK57099.1"
                     /translation="MEVKDFLIVKRNVKYPRIELKGKFVLILPLKGNFRPEEIIEKHR
                     VWIEKKIEFIEKIKGKYKDKKIYKRDDEELKRIVLKYIKRYSEKMKVKVKKIYFRDMN
                     SKWASCRRNGSLSFNLKLKYLPKSLIKYVVFHEMVHILIPNHKSIFWDYIKKEFKNPE
                     KYEEMLYGYWFSLFG"
     gene            6030..6398
                     /locus_tag="PLF90_07205"
     CDS             6030..6398
                     /locus_tag="PLF90_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOK57100.1"
                     /translation="MRWRHGCRWMAFGSGDLLGVLILTILLKKPTYGYSLMDELKNLG
                     IDITFLHYTIVYRTLRMMEISGLVISTWDTTGTGPARRIYSITETGREFLKNWYEWAK
                     KDLKIMENIINEIEKNMERR"
     gene            6403..6636
                     /locus_tag="PLF90_07210"
     CDS             6403..6636
                     /locus_tag="PLF90_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOK57101.1"
                     /translation="MCRRGRGFGFGGWTPPYPYIGIGRGGLPRCAWPGFWYGYTPDIT
                     PEEEIKYLKEEAEFLKKQLSEIEKRIKEIEREK"
     gene            6637..7350
                     /locus_tag="PLF90_07215"
     CDS             6637..7350
                     /locus_tag="PLF90_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK57102.1"
                     /translation="MEIKVIFDKDTDEKFLQTGWGVSFLIEEKILFDTGEKGEWLINN
                     FKKLKVDINKIEKVVISHEHWDHTGGLWDLLKIKKGIEVYGCKGFSEKFKENVKKEGG
                     NFIEIEKLIEFEEKIFTTGEIEGYYKGKKIVEQSLIVKGKNDISVLTGCAHPGILKII
                     DYIRDFFPDESIYAVIGGFHLIEEEKRIVEIIVEEFKKRNIKKVGPTHCTGVLGIELF
                     RERFKENFLEIKVGQKIEV"
     gene            complement(7355..7855)
                     /locus_tag="PLF90_07220"
     CDS             complement(7355..7855)
                     /locus_tag="PLF90_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HOK57103.1"
                     /translation="MKKIIAVCGSNVGDKNLDAGVLKIAEKVGSLIAEKGGILICGGL
                     GGVMEAVAKGAKKKEGITVGILPYDKSKANKYIDIPIGTNLGFYRNYIIVNSADCVIG
                     ICGRWGTLNEISMAIALGKPVILISSSKGICELLSKKEILEKFEEKPYIVETPEEAIE
                     LAFKLI"
BASE COUNT         3211 a          752 c         1441 g         2520 t
ORIGIN      
        1 ttaggccttg aagttgaaat aagtaaattt aaaaaacgag tgataaaatg tccaaattgt
       61 ggactgaaat attataaacc tatagaaaaa caaacagatg ttaaaattgc aacaaaaatt
      121 tttgagattt tttataagaa gatttcggaa attttagtaa taatatcagg agatactgat
      181 ttagtccctg ctatcagaac tacaaaagaa acttttgatg atataaaaat aggcgttgtt
      241 attccatata aaaatagcag tgaagaatta aaattggttt cagattttta ttactcaatt
      301 ccacccagaa aatatattaa tcatttatta ccaaaccctt ataaactaaa aacaggagaa
      361 gtaatatgta aaccagaaaa gtggtaaaaa gggggttctt ttatgaaaaa tctatttttt
      421 gaatattata aaattgatga ggaagaatgg aataaaattt ctaaaaattc ttcacaaaaa
      481 tttaaaaaat tattctggaa aatcccttat gaaaagtatc aggttttact ggatgatgat
      541 attgaaaaag tatacaagaa gggaaaaata aatgaattag ttaaggcact gatgaacaaa
      601 atgggtactt taagaatatc ttatattttc ttaacatatt attacaacct gggaattcct
      661 gatgataaat ggtgtatttc gcctgggaaa aatggttcgt ctattcagta tttccctgat
      721 tttgaagaga agcattttta tattcattat ttttttaatt tctgggcaga ggttttttat
      781 atcaaattct tttcaataat agatattatt tgtgatttac tcaacaatta ttatgaaatg
      841 ggtattaaga aggagacaaa aggttttaga agaaaagtaa taaagaattt aaaaaaggaa
      901 aatcttgagt tatttaagtt tttggaaaat ataaaaaata aaagggaata caaaaatgca
      961 aaagagttcc ggaatagaat tattcattct attccggctt atgaaatttc aaatcaaaat
     1021 gaaataaaga aatatgaaaa taaaattgtt gaaattaaat ttggtgcagg taaatatact
     1081 aaatcaagtg aaattaaaga aaatgcagaa gaagtattaa ttttgctaaa aaatatattt
     1141 gaaaagttaa aagaaaattt aaattttgat gattgagtaa aaatgattat gaattggaat
     1201 tttttgccaa tacttgaagt ttgtgaattt gaaaaaggga ctgaacccgg gagtgagtcg
     1261 tataatagag atgggatggg tattcctttt ataagggtag ggaatattgg tgctaaaatt
     1321 caagaacaga tttacacaac atctgaaaat gtgaaaatat gtaaaaaaga tgatatttta
     1381 attgcacttg atggaagtcc aggaattgta ataagaggat tagaaggtgc ctattcttca
     1441 ggtattagaa aagttattgt aaaagatagt actaaattaa tgaaggaatt tgtttattat
     1501 tatttacaga cggatgaagt tcaaaaaatt attaatgaac atacgatagg agtgacgata
     1561 aaacatgctg gaaaatcttt aaattttata aatattccta ttccgcctat ttcagttcag
     1621 aaaaaaatag ttaaaatact tgatactgtt cagaagggga tagaaattca ggaaaaaata
     1681 attgaaaaga caaaggaaat taaaaaatca cttatggcag accttttcaa atatggagct
     1741 ccctcattca gaaagaacag aaaatcaaag aaaacagaaa taggagaaat acccgaagat
     1801 tggaaagtag tgagattggg agaggtgtgt gagaaaattg agcaaaaaaa tcctgcgaat
     1861 gaaccattga aaaaaatagt atatatagat gtttccagtt tagataatga atattttaaa
     1921 atagtagagc ataaggagta tttaggaaaa gaagcaccaa ctcgtgcaag aaaaattatt
     1981 aaattcggtg atgttatttt tgcaacagtt agaccttatt tgaaaagaat tgctttagtt
     2041 cctgaaaatt ataataatgc ttattgttct actgcttttt gtgtaattag acctattaaa
     2101 gaaaaacttt taagtggttt tatattttat tttttaacat tagataaatt tgttcaaaat
     2161 gtatctacaa atcaacatgg ttccggttat ccagcaataa ctgacaatca tatatacaaa
     2221 gtgaaaatac ccctaccttc cttgtctgaa caaaaagaaa tagcagaaat attgagcgta
     2281 atagatgaaa agattgagat tgagaaaaag aaaaaggaat tatacgagga attgtttaaa
     2341 tctcttttga ataaaataat gaatggagag atagatgttg aaaagattga attgtaaaga
     2401 aggaagaagg agtgaaagat ggaaaaatat gaaaagaaac tggttgaaga ttatgtaagt
     2461 gaaagattaa aagaaattgg ctggaagttt gttagttatg aaaatttaaa aagggaaagt
     2521 ataaaagaac cgttattagt tgaaaattta aaagaggcga tcataaaaat taataaagat
     2581 aaaggtattg gagaagagga aattaaaaaa gtaattgatg aaattaaact cctttcaaat
     2641 catcaggaag gaattaaaag ttttcttcat tatctcaaat atgggattgg aataaagttt
     2701 gaaaaagaaa gagtcgttaa gattattgat ttaattgatt atgagagacc agaaaataat
     2761 gaatttatct tttcaagaca ggttcgtttt aagggttctg atttgattat tcctgatatt
     2821 gtcctttata taaatggaat tcctatagtt gaaattgaat gtaaaagtcc tattgattta
     2881 aaaactgatt ggctaaccgg atatgaacag attaaaaatt atgaaaaaat tatgcctgaa
     2941 ctttataaat atgtgcaaat aggaatttct ttttgtgaaa aagtgaggta ttttcccata
     3001 gttccgtggc aggacaaagt agatgtgtat atctggaaaa aagatgaatt acccgaagat
     3061 gaggcaattt ttgaattttt aaatccatat attttacttg atattttaag gaattttctt
     3121 tttataagag aagtacatgg agaaattaaa aaagttatat gtcgctatat gcagtataag
     3181 gcagtcaata aaatttataa tagggttgtt gataacttaa aaggaaaaga gacaaaaaat
     3241 aaaggattag tatggcattg gcagggttct ggtaagacat taacaatgat ttttgcgact
     3301 cataaacttt attttgaaaa atatcttgaa aatccaacaa tttttattgt tgttgataga
     3361 gaagaacttg aggaacagat gaaagaagaa ttatcatctt tgaaacttaa tttttctttt
     3421 gaagttattg aaagtgttag aaatttaaag gaggttataa ctcatgataa ttattcagga
     3481 aaaagagggg tatttttaac attaattcat aaatttagac ctgatgaaaa atttcttcct
     3541 gatggattac ctgattcaat tgctcatagg aaaaatgtaa tctgttttct tgatgaggtt
     3601 cataggactc aatatggtct tcttaaaagt tgtatgaaaa atattcttaa aaatgctttc
     3661 tattttggat ttacaggaac tccaatcgca gaaaatgaaa gaaatactta taatgaattt
     3721 ggttatccat tggaagatga aggttatctt gataaatatt tccttgacca atcacaaaaa
     3781 gatggtttta ctctaccatt agtttatgaa atcagattag agcatttaca tattcccgaa
     3841 gaagatttta agttttttgt tgaaaagtgg gaagaggatg aatttaaagg agatattgat
     3901 ttacatccag aaataagtgt gaaagttcgc aaaaagttaa atcatatcaa tctttttctt
     3961 gaaaaagagg atagaattga tgagatatgt aaggatatag ggaagcattt taaggaaaat
     4021 atagatggta aatttaaagg aatggtcgta tgtggaagta gaaaagcgtg tgttatttac
     4081 aaaaaatatc taaataaata tttaccgcct gaatattcag aagttgttat gagttttaaa
     4141 attgatgaaa aagaacctat aaactcattt tattatgaat ggaagaaaag gtattcagga
     4201 tttgcaaatg acaaagaaag aattaaaaag ataatagatg actttaaaga aaaggaatat
     4261 ccgaaaattt tgatagttac tgatatgctt attactggtt ttgatgctcc aattttacat
     4321 gttttatatc ttgataaatt attaaaaaaa caccgacttc tacaaacaat tgcaagaaca
     4381 aatagacctt atggagatgt taaagaatgt ggatttatta ttgattatgc tggagtaata
     4441 aaaaatctga aatatgcttt aaaagaatat tacaaaagcg aaataaaagg acttataact
     4501 gagtatgata aactttttga agagtttgaa aaatgtattg aaaatatgaa agagattttt
     4561 aagaatatta agtttaaaat tgaaagggaa gtccttttga aatctttgga tattttaaga
     4621 gatgacaaaa taagagatag attttttgag aattataaaa aaacaaggaa gatttttgaa
     4681 attctcgctt ctcatccaga aaaattgaag tacttaaaag aatttaaatg gttttctgct
     4741 gtttatgatt attacagaaa attgactgaa gatgaggaag aaaaagaact tgttgataaa
     4801 ttttttaaaa aaacggttga ttttgtccat aaaaatttaa aagtaaaaga aataaaaaaa
     4861 acactgccaa gtttaaaatt agatttgaaa acaattgaag agattagaaa aagtgctatt
     4921 cctgaaaaag aaaaggttgt gaatattatt tttattcttt caaaacttgt ttatgttgaa
     4981 agaggaaaaa atcctgttta tagaacaatt gctgatagaa tagatgacct gataagaaaa
     5041 tggaaggaaa ggaagattga aattgaggag ttatttaagg aagagaaaga aattttgaag
     5101 gaaatagaaa taaaagaaaa agagaaagaa aaacttgaat ttgataattt tgactatggg
     5161 ctttatttaa ttttaaaaga tgagataaaa gaagatgaaa ttacacttaa aaattatgtt
     5221 gaagagataa aaaatggaat aaaagatagt atggttgaaa actggattga aaacccagcg
     5281 ttaagacagg taattgagag aaaaacaaga gagatatgtc ttgagataaa aaataaacat
     5341 aatttagatt atgaaaaatt tgatttatta cacaggaaaa ttattgaatt tgtgaaggaa
     5401 tatggaagtt aaggattttt taattgtaaa aaggaatgtt aaatatccaa gaatagaact
     5461 taaagggaaa tttgttttaa ttctgccact taaaggtaat tttagaccag aagaaataat
     5521 tgagaaacat agggtttgga ttgaaaagaa gattgaattt atagaaaaaa taaagggaaa
     5581 atataaggat aaaaagattt ataaaaggga tgatgaagaa ctgaaaagaa ttgttttgaa
     5641 gtatataaaa agatattctg aaaaaatgaa agtaaaagtt aaaaaaattt atttcaggga
     5701 tatgaattca aaatgggcaa gttgtagaag aaatggaagt ttgagtttta atttgaaact
     5761 aaaatactta ccaaaatctt tgataaaata tgttgttttt catgaaatgg ttcatatttt
     5821 aataccaaat cataaatcaa ttttctggga ttatataaaa aaagagttta aaaatcctga
     5881 aaaatatgag gaaatgcttt acggatattg gttttctttg tttggctaat ccttcctaat
     5941 ttaaattttt aatttttttt atttaactat tgacaatttg cacatatgtg ggtataataa
     6001 atatagaaaa tttgaaaata ggaggaaaaa tgaggtggag acatggttgt agatggatgg
     6061 catttggttc aggtgattta cttggtgtat tgattttaac gattttattg aaaaaaccta
     6121 cttatggata ttctttaatg gatgaactta aaaatcttgg aattgatatt acatttttac
     6181 actatacaat tgtttacaga actttaagga tgatggagat ttcaggactt gtaatttcaa
     6241 catgggatac aactggaaca ggacctgcaa gaagaattta tagtataact gagacaggaa
     6301 gagaattttt gaaaaactgg tatgaatggg caaaaaagga cttaaaaatt atggaaaata
     6361 taataaatga aattgaaaaa aatatggaaa ggaggtgaga atatgtgcag aagaggtaga
     6421 ggatttggat ttggtggctg gactcctcct tatccctata ttggaatagg aaggggagga
     6481 cttccaagat gtgcatggcc tggtttttgg tatggatata caccggatat aacacctgag
     6541 gaagagatta aatatttaaa agaagaggca gagtttttga aaaagcaact atctgaaata
     6601 gagaaaagaa taaaagaaat tgaaagagaa aaataaatgg aaataaaagt tatttttgat
     6661 aaggacacag atgagaagtt tttacagaca gggtggggtg tttcttttct tattgaagaa
     6721 aaaattcttt ttgatacagg agaaaaagga gaatggttga ttaataattt taaaaaattg
     6781 aaggttgata taaataaaat tgaaaaggtt gttatttctc atgagcactg ggaccataca
     6841 ggtggcttat gggatttatt aaaaattaaa aaagggattg aagtttatgg atgtaaagga
     6901 ttcagtgaaa aatttaaaga aaatgtgaaa aaggaagggg gcaattttat tgaaatagaa
     6961 aaactgatag aatttgaaga aaaaattttt accacaggag agatagaagg atattataaa
     7021 gggaaaaaaa ttgtagaaca gtcattaata gtaaaaggaa aaaatgatat ttcagttctt
     7081 acaggttgcg ctcatccagg aatattaaaa ataattgatt atataagaga tttttttcct
     7141 gatgagagta tttatgcagt tataggtggt tttcatttaa ttgaagagga aaaaagaatt
     7201 gttgagatta tagttgaaga atttaaaaaa agaaatatta aaaaagtcgg accgactcac
     7261 tgtacaggtg ttttaggcat tgagttattc agggaaagat ttaaagagaa ttttttagaa
     7321 ataaaagtag gtcagaaaat agaagtgtaa agaattaaat aagtttaaag gcgagttcaa
     7381 ttgcttcttc tggggtttca actatatatg gtttttcttc aaatttttca agtatttcct
     7441 tcttacttaa taattcacat ataccctttg aagaagagat taaaattaca ggttttccaa
     7501 gagcaatagc cattgatatt tcattcaaag ttccccatct tccgcatatc cctattacac
     7561 agtcagcact gttaacaatt atataatttc tataaaaacc aagatttgtt cctattggta
     7621 tgtctatata tttatttgct tttgatttat cataaggaag aatgccgaca gttattccct
     7681 cttttttctt tgctcctttt gctactgctt ccataacccc accaagtcct ccacagataa
     7741 gaattccacc cttttcagca attaaagagc caactttttc agcaattttt aaaactcccg
     7801 catctaaatt tttatcacct acattacttc cacagacagc aattattttt ttcattttaa
     7861 taaagaaaac gcccccagcc ggactcgaac cggcgctacc accttgaaag agtggtgacc
     7921 taga
//
LOCUS       DAOSAT010000080         7856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_5984_length_7856_cov_5.508909, whole genome shotgun sequence.
ACCESSION   DAOSAT010000080 DAOSAT010000000
VERSION     DAOSAT010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7856
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5984_length_7856_cov_5.508909"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLF90_07225"
     CDS             <1..244
                     /locus_tag="PLF90_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK57104.1"
                     /translation="PLTEEENARTEELREILNEAINSLPPKYKTIFILHDIEGLSLSE
                     ISEILSLSIPTIKTRIHRSRLYLREKLSEYFKRKGN"
     gene            246..479
                     /locus_tag="PLF90_07230"
     CDS             246..479
                     /locus_tag="PLF90_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOK57105.1"
                     /translation="MDCREIIKLISDYIDKEIEQTKKEILEEHIKICKKCESILNTTE
                     KTLFLSRKIYKNKKIPKKIEKTLYYQIRIRYKK"
     gene            540..980
                     /locus_tag="PLF90_07235"
     CDS             540..980
                     /locus_tag="PLF90_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOK57106.1"
                     /translation="MAKLARWNPIKEVLDLRDDFDRLIDRFFSKDFDIWEGPRPFDVD
                     IYEEADNVVVKAEIPGMNKEDITVSLTEDTVTISGKKAEEKKIEKENYFRKEIRTGSF
                     SRSFTLPCAVDKEKVKATYKNGILEIVLPKSEKAKEKEIKVEVE"
     gene            1091..2005
                     /gene="trxB"
                     /locus_tag="PLF90_07240"
     CDS             1091..2005
                     /gene="trxB"
                     /locus_tag="PLF90_07240"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOK57107.1"
                     /translation="MKKVITIGAGPAGLSAGIFLCRDKIPTLIIEKLSPGGNMLLTEI
                     IENYPGFPDGISGFALAENMRKQFLKFGGEIKTGEVEKIELKDNEKIVIMRNGEILKC
                     DVLIIASGSSRKKLNVKGEEELMGKGVSYCAICDGAFFRNKDVAVVGGGDSALEESIY
                     LTRFANTVYLIHRRENLRGKRYLQEEVKNNKKIKLILPYIVREIKGKEKVEKLILQNT
                     KNEEIIDLKVDGIFISAGQKPNTEFLNGIVKTDDNGFIIVNEKMETNVPGIYACGDVI
                     KKSLYQIITAASEGMIAAFFAEKYLEKL"
     gene            complement(2000..2965)
                     /locus_tag="PLF90_07245"
     CDS             complement(2000..2965)
                     /locus_tag="PLF90_07245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK57108.1"
                     /translation="MKIPLPYSGGIILSYQCNLSCIHCLYNCRKNWMEWIDIDKFEKC
                     LKILKNISSFVSLHLTGGEPFINFPLLLEAVKLTKKYEIPFFVETNCYWAKNEEVAEE
                     KLKKIKEGGLNSIMLSYSVFYLNKVPVENFEIAMKKSFEIFGMENVSFYTIDAYRIIK
                     EKGIRGTMNFEDCLKIIGQEKFINFLKNYIVPKGKFLYTLENWFVKFKAEKFKGANCI
                     GEFLNPYHIHIDLYGNYITSFCSGITPGKFEEVLKNGINTGDKILNFLMTDIYDLLLW
                     AESYGYKRKDGYINKCHLCLDIRKFLIENKFKFDSLKPEEFYNYL"
     gene            complement(2962..3525)
                     /locus_tag="PLF90_07250"
     CDS             complement(2962..3525)
                     /locus_tag="PLF90_07250"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018759450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOK57109.1"
                     /translation="MVLMIDNYDSFVYNLVQYFGELGEKIIVYRNDVSIKKIERIKFD
                     RIVISPGPKRPENAGISCEIIEKFKDKFPILGVCLGHQCIGYVFGGKITYAKKLMHGK
                     TSLIYHNGKDIFEGIENPFVATRYHSLVIEKESLPNSLEITAWTEDDEIMGVRHKEYK
                     IFGVQFHPESILTEEGKKILKNFLEIK"
     gene            complement(3528..5006)
                     /gene="trpE"
                     /locus_tag="PLF90_07255"
     CDS             complement(3528..5006)
                     /gene="trpE"
                     /locus_tag="PLF90_07255"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOK57110.1"
                     /translation="MEIFPDFEEFKSLSKKVNVIPFHTWFLADFETPVTSFLKIAKGS
                     YNFLLESAEQEEKTGRYSFIGSYPEIIIKGKENRVEITDYTKGIIEKFEEKNSLKIID
                     DFLKKYSTVKIKPLPSFTGGFVGFVSYDYVRNIEKIPQICKDDLSIPDFIFFFPKKLI
                     IFDHFKRKIFLITNVFVEKNKTLKQIYDEGKENLLKFTGEFFKSFKVIERKVEEKNDD
                     FKSNFKKEEFLKNVEKVKKYIFEGDIIQAVISQRWEREIDTEVFKIYRALRSLNPSPY
                     MFYLELGDLKLIGSSPEILVKLENGIANVRPIAGTRPRGKDEKEEKKLEEELVNDEKE
                     KAEHIMLVDLGRNDLGKVCYPGTVRVTELMKIEKYSHVMHLVSNVEGKLMKNKSSVDL
                     FNSTFPAGTLTGAPKIRAMEIIEELEGVRRGPYGGAVGYFSLQKSMDFCITIRTFIIK
                     GKKIYIQAGAGIVADSVPEKEYEETENKAKALIESYNFSKRI"
     gene            complement(5010..6572)
                     /locus_tag="PLF90_07260"
     CDS             complement(5010..6572)
                     /locus_tag="PLF90_07260"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOK57111.1"
                     /translation="MKRKQVLVIFLFFFFFLIKESEPFLIKKPEEKARRYFKLSLEEY
                     RKAIKQNPNNNEIIEEYIKILESTVGKTEAKIELALIFKEINLQPRVSEILLEISLFD
                     RKNALSYIEKRIEKSQDIREKINLYYIATELSPQNPIYWYNLGKLLLGINREEEGISA
                     LEKAYNYNLKDVSLFYYLSLYYLNKGDYKKAKEYIEEGLAISDDINLHKILLKIYLAQ
                     NNKGMAISEREKIKNLLAVREEKKIEEKKEEKITKKEKIEGLNPYSFINVSKNEQKLY
                     IVSFDGYTFKEIKEFKCTTGTEKGDKEKKGDKRTPEGTYLLTSKIEPPNLPSKYGIAA
                     FPLNYPNPIDLRLNKDGNGIWFHATPIERPPYNSEGCIVVNDGDMKQIMPYIKTGETF
                     ICISQDKLFNKFEYIKEIKETVERWKNAWESKNIEEYIKFYDDEFLTGGKNKKEWRLY
                     KERINKGKKYISIELSDLQILPYGEKKFGLMAISFFKQNYTSDNFSSKTRKILYLVKR
                     NGNWKIICEQTL"
     gene            complement(6584..6769)
                     /locus_tag="PLF90_07265"
     CDS             complement(6584..6769)
                     /locus_tag="PLF90_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOK57112.1"
                     /translation="MKVRIDKELCTGCGLCVDICPEVFEMGETVSTVKLTNVPSELEE
                     KVKEAAESCPVEAIIIE"
     gene            complement(6833..6904)
                     /locus_tag="PLF90_07270"
     tRNA            complement(6833..6904)
                     /locus_tag="PLF90_07270"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6869..6871),aa:Gln,seq:ctg)
     gene            complement(6920..7126)
                     /locus_tag="PLF90_07275"
     CDS             complement(6920..7126)
                     /locus_tag="PLF90_07275"
                     /inference="COORDINATES: protein motif:HMM:NF022229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOK57113.1"
                     /translation="MITEIGIVAGEIWQYLEKYNETTLTKLIEITGRDRDLLIMGLGW
                     LAREGHIILTKKDTDYRIVLRKKD"
     gene            complement(7153..7380)
                     /locus_tag="PLF90_07280"
     CDS             complement(7153..7380)
                     /locus_tag="PLF90_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57114.1"
                     /translation="MIKVSIDLIASIYLLISVCILFMWIFLEKRRKYEKTEENTLWCC
                     PICFYEYVDSKSNDISRCPRCKTLHKKGEKR"
     gene            complement(7377..>7856)
                     /locus_tag="PLF90_07285"
     CDS             complement(7377..>7856)
                     /locus_tag="PLF90_07285"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOK57115.1"
                     /translation="IGSQKIKTEEEVPSMNIKNITSKDIVGEKEYITPVKLDLDVEKA
                     EYKISSLSLPVPVLNLPQNNPSEDLIEGPAGSRKIIYREKIDYPLWAQQKGIEGKVKI
                     KFWVNPEGKIFNTEISLSSGNPEIDFYAERKFKKWLFESVESEKDVWGIITLVFKLK"
BASE COUNT         2657 a         1324 c          978 g         2897 t
ORIGIN      
        1 accgttaacg gaagaagaaa acgcaagaac tgaagaattg agagagattt taaacgaagc
       61 aataaattcc ttacctccga aatataaaac tatttttata ttacacgata tagaaggact
      121 ttcattatca gaaatatcag aaattctttc actatcaata ccaactataa aaacaagaat
      181 tcacagaagc agattgtatt taagggaaaa actatcagaa tattttaaaa ggaaaggaaa
      241 ttaaaatgga ttgcagagag attataaaac ttatatctga ctacattgat aaagaaatag
      301 aacagacaaa aaaagaaata cttgaagaac atattaaaat ctgcaaaaaa tgtgaatcta
      361 ttttaaatac tactgaaaaa accttattcc tttcaagaaa gatttataaa aataaaaaaa
      421 tcccaaaaaa aatagaaaag acactttatt atcaaataag aataagatat aaaaagtgaa
      481 accaacctcc ttttctgctc atctaaaaaa tgaaaaaaca ggaaaggagg tgatgaaaaa
      541 tggcaaaact cgcaagatgg aatccaataa aagaagttct tgatttaaga gatgattttg
      601 acagattaat tgatagattc ttctcaaaag attttgatat ttgggaagga ccgagacctt
      661 ttgatgtaga catatatgag gaggcagata atgttgttgt aaaagcagaa atcccgggta
      721 tgaataagga agacatcact gtttcactga ccgaggatac agttaccata tccgggaaaa
      781 aagcagagga aaagaagata gaaaaagaaa attatttcag aaaagaaatc cgcacaggaa
      841 gtttctccag aagttttact cttccatgtg cagtggataa ggaaaaagta aaagccactt
      901 acaaaaatgg catccttgag atagtccttc ccaaatcaga aaaagcaaag gaaaaagaaa
      961 taaaggtaga agttgaataa taactattct gaaaaagggg ggcttttccc ccttttttat
     1021 tttattagaa atattaccca cttaaataat ttttgaaaaa cagaaaatat ttggtgtaaa
     1081 ataaacggaa atgaaaaaag taataacaat tggtgctgga cctgccgggc tttctgcggg
     1141 tatttttttg tgcagggata aaatcccgac attaattatt gagaaacttt ctcctggtgg
     1201 aaatatgctt cttacagaaa ttatagaaaa ctatcccggt tttcctgatg gaataagtgg
     1261 ttttgcgctg gcagaaaata tgagaaaaca atttttaaaa tttggtggag aaataaaaac
     1321 aggagaagtt gaaaaaattg aattaaaaga taatgaaaaa atagttataa tgagaaatgg
     1381 agaaatatta aaatgtgatg ttcttataat cgcctcaggt tcttcaagaa aaaaattaaa
     1441 tgtgaaggga gaagaagaac tcatgggaaa aggagtatct tactgtgcaa tatgtgatgg
     1501 tgctttcttc aggaataaag atgttgctgt tgttggtgga ggagattctg cacttgaaga
     1561 aagtatatat ttaacaagat tcgcaaatac agtttatctt atacacagaa gagaaaattt
     1621 aagaggaaaa agatacttac aggaagaagt aaaaaacaat aaaaagataa aacttatatt
     1681 accttacatt gttagagaaa taaaaggaaa agagaaagtg gagaaattga ttttgcagaa
     1741 tactaaaaat gaggaaatta ttgatttaaa agttgacggt atttttattt cagcaggaca
     1801 aaaaccaaat acagaattct taaatggaat tgtaaaaacg gatgataatg gatttataat
     1861 agttaatgaa aaaatggaaa caaatgtacc tggaatttat gcatgtggag atgtaattaa
     1921 aaaatcatta tatcagataa taacagcagc aagtgaagga atgattgctg ctttctttgc
     1981 agaaaaatat cttgaaaaac tataggtaat tataaaattc ttctggtttg agcgaatcaa
     2041 atttaaattt attttcaatt aaaaatttcc ttatatcaag acatagatga cacttattaa
     2101 tataaccatc ctttctttta taaccataac tttcagccca taaaagtaaa tcataaatat
     2161 cagtcataag aaagtttaaa attttatcac ctgtatttat tccatttttc aaaacttctt
     2221 caaattttcc aggagttatt ccagaacaaa aacttgttat ataatttcca taaaggtcta
     2281 tatgaatatg ataaggatta agaaattcac caatacagtt tgctccttta aacttttctg
     2341 ccttaaattt aacaaaccag ttttccagtg tatacaaaaa ttttcctttt ggtacaatat
     2401 aatttttaag aaaattgata aatttttcct gtccaataat ttttaaacaa tcttcaaaat
     2461 tcatggttcc ccttataccc ttttctttaa taatcctgta tgcatcaata gtgtaaaaac
     2521 tcacattttc cattccaaaa atttcaaaac tttttttcat ggcaatttca aaattttcaa
     2581 caggtacttt attgaggtag aatactgaat aacttaacat aatagaatta agtcctcctt
     2641 cttttatttt ttttaatttt tcttcagcaa cttcttcatt ctttgcccag taacaatttg
     2701 tctcaacaaa aaacgggatt tcatattttt ttgtcaactt tactgcttca agcaaaaggg
     2761 gaaaattgat aaatggttct cctcctgtta aatgcaagga gacaaaagaa gatatgtttt
     2821 tcaatatttt taaacatttt tcaaatttat ctatatctat ccattccatc cagtttttcc
     2881 tacaattata taagcagtgt atacaagata gattacattg atatgaaagt ataatacctc
     2941 ctgaataagg aagaggtatt ttcattttat ttccaaaaaa ttttttagaa tttttttccc
     3001 ttcttctgtt aaaattgatt caggatgaaa ctgtacacca aaaattttgt actctttatg
     3061 tcttaccccc atgatttcat catcttctgt ccatgctgtt atttcaagag aattgggtaa
     3121 actttctttt tctattacaa gtgaatgata tcttgtagca acaaaaggat tttcaattcc
     3181 ctcaaaaata tcttttccat tatgataaat taaagatgtt ttcccgtgca taagtttttt
     3241 agcataggta atcttgcccc caaaaacata tccaatacac tgatgtccaa gacatacccc
     3301 gagtatagga aatttatcct taaatttttc tattatttca catgaaattc ccgcattttc
     3361 aggtctttta ggtcccggag aaataacaat tctatcaaat tttatccttt caattttttt
     3421 aatactcaca tcatttctat aaactataat tttttctcca agttctccga aatactgaac
     3481 aaggttataa acaaaagaat cgtaattatc tatcatgagt accattttta tatccttttt
     3541 gaaaaattat aactttcaat aagtgctttt gctttatttt ccgtttcttc atattctttc
     3601 tcaggaacag aatcagcaac aattcctgca cccgcctgta tatatatttt tttaccctta
     3661 attataaatg tccttatagt tatacagaaa tccatactct tctgtaaact gaaataacca
     3721 acagcacctc cataaggccc ccttctaact ccctctaact cttctataat ctccattgct
     3781 cttatttttg gcgctcctgt taatgtacct gctggaaaag ttgaattgaa taaatcaaca
     3841 gaacttttgt ttttcatcaa tttaccttct acatttgaaa caagatgcat aacatgagaa
     3901 tatttttcta ttttcatcaa ctctgtaacc ctaacagttc ctggatagca aaccttaccg
     3961 aggtcatttc ttccaaggtc aacaagcata atatgttctg ctttctcttt ttcatcattc
     4021 accaattctt cttcaagttt tttttcttct ttttcatctt ttcctctcgg tcttgtccct
     4081 gcaattggtc tgacatttgc aattccattt tcaagtttta caagtatttc aggagaagaa
     4141 ccaatcaatt ttaaatctcc aagttcaaga taaaacatat atggagatgg attcaaactt
     4201 ctcaatgccc tgtatatttt aaaaacctct gtatcaattt ctctttccca tctctgagaa
     4261 ataaccgcct gaattatatc tccttcaaaa atatattttt tcaccttctc aacatttttc
     4321 aaaaattctt cctttttaaa attgctttta aaatcatcat ttttctcttc aactttcctt
     4381 tcaattactt taaatgattt aaaaaattca cctgtaaatt ttaaaagatt ctcttttcct
     4441 tcatcataaa tctgttttaa agttttattc ttttcaacaa atacatttgt tattaaaaaa
     4501 attttccttt taaaatggtc aaaaattatc aatttctttg ggaaaaagaa aataaaatca
     4561 ggaattgaaa ggtcatcctt acatatctgt ggaattttct ctatatttct cacatagtca
     4621 taagaaacaa atccaacaaa accacctgta aaagaaggaa ggggtttgat tttaactgta
     4681 gaatattttt ttaaaaagtc atctattatc tttaaagaat ttttttcctc aaacttttca
     4741 attatccctt ttgtataatc tgtaatttct actctatttt cctttccctt gattattatt
     4801 tcaggatatg agccaataaa agaatatcta cctgtttttt cttcttgctc agcgctttca
     4861 agaagaaaat tataacttcc ttttgcaatt tttaaaaaag aagtaacagg cgtttcaaaa
     4921 tcagcaagaa accatgtatg aaaaggaatt acatttactt ttttactcaa acttttaaat
     4981 tcttcaaaat caggaaatat ctccattttt taaagtgtct gttcacagat tatcttccaa
     5041 tttccatttc ttttaacaag ataaagaatt tttcttgttt ttgaagaaaa attatctgat
     5101 gtataattct gtttgaaaaa agagattgcc atcagtccaa atttcttttc cccatagggt
     5161 aaaatctgta aatcagaaag ttcaatactt atatattttt ttcctttatt aattctttcc
     5221 ttgtataatc tccattcctt tttattcttt cctcctgtta aaaattcatc atcataaaat
     5281 tttatatatt cctctatatt tttactttcc catgcatttt tccatctttc cactgtttct
     5341 tttatctcct taatatactc aaatttatta aacaatttat cctgtgaaat acagataaaa
     5401 gtttctcctg ttttaatgta tggcattatc tgtttcatat caccatcatt aacaacaata
     5461 caaccttcac tattgtaagg cggtctctct attggtgtag catgaaacca tataccattt
     5521 ccgtctttat tcaatcttaa atctattgga tttggataat taagaggaaa ggcggcaatc
     5581 ccgtattttg aaggtagatt tggcggttct atttttgatg ttaaaaggta agtaccctca
     5641 ggtgtccttt tatctccttt tttctcctta tccccttttt ctgttccggt agtacattta
     5701 aattctttta tttctttaaa agtatatcca tcaaatgaaa caatatataa tttctgctca
     5761 ttttttgaca catttataaa gctatatgga tttaaacctt caatcttttc ctttttcgtt
     5821 attttttctt cttttttctc ctctattttc ttttcttctc taacagcaag taaattttta
     5881 attttttccc tttctgatat cgccattcct ttattatttt gagcaagata tattttcaga
     5941 agtattttat gaagattaat atcatcagaa attgcaagtc cttcttcaat atattctttt
     6001 gcttttttat aatctccctt attaagataa taaagggaaa ggtaataaaa aagggataca
     6061 tcttttaaat tgtaattata tgccttttca agtgctgaaa ttccctcttc ttctctatta
     6121 atccccagaa gtaatttacc gagattatac cagtaaattg gattttgagg agaaagttct
     6181 gttgcaatat aataaagatt aattttttcc cttatatcct gacttttttc tattctcttt
     6241 tctatataag atagcgcatt tttcctgtca aaaagcgaga tttcaagtaa aatttcagaa
     6301 acccttggtt gtaaatttat ttctttaaaa attaaagcca gttctatttt tgcttctgtc
     6361 tttccaacag tactttccag aatctttata tattcttcta ttatttcatt attatttgga
     6421 ttctgtttta tcgcttttct gtattcttca agagaaagtt taaaatatct tctcgctttt
     6481 tcttctggct tttttattaa aaatggttca ctctctttaa taagaaaaaa gaaaaaaaat
     6541 agaaaaataa caagtacctg ttttcgtttc attcaactct ttttcattct attattattg
     6601 cctctacagg acaactttct gcggcttcct ttactttttc ctcaagttca gaaggaacat
     6661 ttgtcaattt tacagttgaa actgtttcac ccatttcaaa aacttccgga cagatatcaa
     6721 cacataaccc gcaaccagta cagagttctt tatcaattcg tactttcatt ctttcctcct
     6781 tcctttttta agatagtaaa ttttatattt ttcttaaaat ttgtcaatat tgctggggga
     6841 ataggactcg aacctatacg ccgggatcca gagtcccgtg tcctaccagt tagacgatcc
     6901 cccagtttaa ttcttcaatt caatcctttt tccttaaaac tattctgtag tcagtatctt
     6961 ttttagtaag aataatatgt ccttctcttg caagccaccc caatcccatt atcagtaaat
     7021 ccctatccct tcctgttatc tcaattaatt tagtcagagt ggtttcatta tatttttcaa
     7081 gatactgcca tatttctcct gccactattc ctatttcagt tatcatttta cctccttgct
     7141 ttttttattt tatcatcttt tttctccctt tttgtgaaga gttttacatc ttggacatct
     7201 gcttatgtca ttacttttac tatcaacata ttcataaaaa catataggac aacaccacaa
     7261 agtattttct tctgttttct catattttct tcttttttct aaaaaaatcc acataaaaag
     7321 aatgcataca cttattaaaa gatagatact tgctataagg tcaattgata cctttatcat
     7381 ttgagtttaa aaactaatgt tataattccc cagacatctt tttcactttc cacagattca
     7441 aataaccatt ttttaaattt tctttcagca tagaaatcaa tttccggatt acccgaagat
     7501 aaagaaattt ctgtattaaa tatttttcct tccggattca cccagaattt tatctttact
     7561 ttcccctcta ttcctttttg ttgagcccac agtggataat caattttttc cctgtatata
     7621 atttttcttg aacctgccgg tccttctatc aaatcttcag aaggattatt ctgtggaaga
     7681 tttaaaactg gtactggtaa tgataatgaa cttattttat attctgcttt ttcaacatca
     7741 aggtcaagtt tcacaggagt tatatattct ttttcgccaa caatatcctt tgatgtaata
     7801 tttttaatat tcattgaagg gacttcctct tctgttttaa ttttttgact acctat
//
LOCUS       DAOSAT010000081         7826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_6014_length_7826_cov_4.677004, whole genome shotgun sequence.
ACCESSION   DAOSAT010000081 DAOSAT010000000
VERSION     DAOSAT010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6014_length_7826_cov_4.677004"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /gene="lysS"
                     /locus_tag="PLF90_07290"
     CDS             <1..1276
                     /gene="lysS"
                     /locus_tag="PLF90_07290"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOK57116.1"
                     /translation="AIRGHGNAAFADIVDSTGKIQVYFKKDIVGEENYEIFKKIDIGD
                     IVGIEGEVFRTKTGEKTILVKNFLILSKSLRPLPEKWHGLKDVEIRFRKRYLDLIMNE
                     SARDIFKKRIMILKFMREFLNDKGFVEVETPMMHPIPGGAEAKPFITYHNTLDMELYL
                     RIAPELYLKKLLVGNFEKIYEINRSFRNEGISTLHNPEFTMLELYSAYGDYEEMIEIT
                     EGLICFLSEKIFGSLTFEYQGKKIELTRSWKRIKYEDVFKEYAGIENFRDEEVIDKKV
                     KEYQIEVEENETLFDKLESIFKKKIQPLLINPTFIIGYPVEISPLAKTFKDSPEITER
                     FELFIGGIEVANAYSELNDPIEQLKRFEEKFKDVKDKEKKIDYDFVEALEYGMPPAGG
                     LGIGIDRLVMLFTNSSSIREVIFFPLLKPKNE"
     gene            1269..2783
                     /gene="murJ"
                     /locus_tag="PLF90_07295"
     CDS             1269..2783
                     /gene="murJ"
                     /locus_tag="PLF90_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOK57117.1"
                     /translation="MSKKIFRAISLLAIGTIISRFFGLFREIVSANFFGTTKIYDAFL
                     LSFMIPNFFRGLLAEGALTSSFIPVFTEYLSDEGEKSQLKEIVDITFTLSLIFTFSLF
                     LIFFILSEIGVRILIYESKWYYVFLLLKFTFPYLIFISLSALYTGILNSYKSFFLPSI
                     SPIVLDFFWILSLFYFAHFFGNTLEEKIFSLCLGVILGGIGQYLILVPSVRKLGYSPK
                     ISLNFSHPAIKKMGFLFLPMIVGVAVGPINLLVDYSLANFLSSGMVSGLWYATRLYQL
                     PLGIFGISISTAVLPWLSENFSKKEFDKFRENLNYAFKILFLTIIPSTIFLIFLKDEI
                     ITFLFKRGMFDVKSVNLTSYPLFFYSLGLVFYGGISVMTRCFYAFNDTRTPVKIGVFS
                     IATNFILDIILMQFLAHGGIALSTSLVGLINFLLLFYFFNKKHLKVDYGKILEIFGVS
                     FISAIPMFIFLTIFRNFLLQKLNLFNFLFLSVSSGLILYFFSFKFISRILRRRI"
     gene            2785..5295
                     /locus_tag="PLF90_07300"
     CDS             2785..5295
                     /locus_tag="PLF90_07300"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HOK57118.1"
                     /translation="MKGIKYEGNKIKQIKFLLGGIGAGNVSVEGKGSLTDWEIFNRPG
                     KGKRMYGNFVSLRVKSKDGIKYRIVSGNPFPPFESAGGYQNLNMEGCPCFERVEFTNY
                     FPFAIIDFSDKRLSLKITLEAFTPFIPLDYENSSLPIAVFTYRIKNLTEKELEIFLAF
                     SIMNPVGTDGTENLNSPKNVCFGGNINIYKEIGEVKGLYLKSNKYEKESHRYGNISLF
                     TDSKNISYKTQWEKIGWWTDYENFWREFKNGKFEFVESKESQNGETYWATLGVKENLK
                     GKEEKEINFYLSWYFPNRINYWGLNEEVKNKILKNWYSKRFSDSAEVVKYYIKNKKYL
                     KDESIKFAESFFSQSLPESFLATISSQFNTLRSNTFFITEDGRFYAFEGCSDNNGCCP
                     LNCTHVYNYDFTVSYLFPNFMKSQREVDYLYNTDENGYMAFRTNIPLGIKLWQFKPAA
                     DGQMGTVLKAYREWKITGDNEFLKRIYTKLKKSVEYAWENWDKNRDGLMEGEQHNTYD
                     VEFYRENPFTSFIYLASLKAMEEISEFMGDIDFKNECKRIYEEGRKKVIEELWNGKYF
                     VQKCKINPLPPYQFLNGCLTSQLLGQFFADNLNLGSIVEEKYIKKTLSSILKYNFIDF
                     SEHINFMRIYGIGNEKGVIVCSFPEGRPEIPMPYCDEVWTGEEFVIASLLIRRGKLKQ
                     AKEIIEAINNRYDGEKRNPFNHIECGYHYGRGFSAFGLILSYLGFFVDNVRKEIYFSP
                     VLLKKELNIFFSTGTGWGNYKYSKKGKLQVVEIEILHGFIEMKNLIFKIDNVKNRKIE
                     IKVDDIKKDFDFVLNRNSVKIFFGKPELILKRIKVLIK"
     gene            5292..6200
                     /locus_tag="PLF90_07305"
     CDS             5292..6200
                     /locus_tag="PLF90_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOK57119.1"
                     /translation="MKKNYITKKTFEKIKDEKFNLLIGANLITITNIQLNFHKINSPT
                     YPHYHPDAQLLYYLKGSGIEEIDGKKFNIVPGSFVFIPSYKKHAFFPYKETTAEIFTM
                     RFEAKKNIIKNSDSSLLSKGFFKILKLFYGKKPDVWTVKEDRKNEVENLIKKIDENLS
                     EKKFGYIISIEGYLLLLINEFLTSYLERREIVEKKASKKEIIFKKVVNFITENIEKQV
                     TIKEIAKNYYFSVNYIQKIIKEFTGRSFTRFMHDIKIEYVKELLKNTTLEIKEIAGRC
                     GFYDQNYFTRIFKKIEGIPPSKYREI"
     gene            6283..7194
                     /locus_tag="PLF90_07310"
     CDS             6283..7194
                     /locus_tag="PLF90_07310"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOK57120.1"
                     /translation="MKKLDGLLPAIVIPYKENYEVDEEVLRKYVEWISKFEIKGLVIN
                     SDAGEGHFLTHEERKRNIKIIKEIIGDKIPLISGLGGQTTKDVIESGKEFKDLGVNYF
                     LVFPHPAFKGGPAKDNLIIKYHEEISKIGIGMIIFQLQEALGGTFYHFETLKELVEIE
                     NVVAIKEATFDALKFKETVDFLNTLSKKIEILTGNDNFIPESFLLGADGALIGFGSIF
                     TDIQVDVIKKIKEKKFDEGLNLFRKIEKICVYCFKRPVRDYRARIKEFLVCMGIFKNS
                     LVRSPLLPLSKEEKEEIRKLYEEFKNG"
     gene            7187..>7826
                     /locus_tag="PLF90_07315"
     CDS             7187..>7826
                     /locus_tag="PLF90_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57121.1"
                     /translation="MDKKKKFEELKKYYLENPIDEKFSEIVKDELGIDLKAKYGNFTL
                     NHPVIVAPGQLTREKGQIERIKKAGYSGCVLKSVVGEDENGNCSMKEYRKKMTYLKTV
                     YEKDDKEGAYPVILWDGRADTRNLTEYMEFARDVYNFSDSKFLIVASILCHLPKPEEE
                     IKKEEWLYTVRKLYDTGYRIFEIDFCPGLKNNKKIIEKENICRLYREIPELLK"
BASE COUNT         2951 a          849 c         1344 g         2682 t
ORIGIN      
        1 ggctattaga ggtcatggaa atgctgcttt tgctgatata gttgacagta caggtaaaat
       61 tcaggtttac ttcaaaaaag atatagtggg tgaggaaaat tatgaaattt ttaagaaaat
      121 tgatatagga gatattgttg gaattgaagg tgaagttttc agaacaaaaa caggagaaaa
      181 aactatactt gttaaaaatt tccttatcct ttcaaaatca ttaagaccct tacctgaaaa
      241 atggcatgga ttgaaggatg ttgagataag attcaggaaa aggtatcttg atttgataat
      301 gaatgaaagt gcaagagata tttttaaaaa aagaataatg attttgaaat ttatgagaga
      361 atttttgaat gataaaggat ttgtagaagt tgaaacacca atgatgcatc caattcctgg
      421 aggtgctgaa gcaaaaccat ttattacata tcacaataca cttgatatgg aactctattt
      481 aagaattgca cctgaacttt atttaaaaaa acttcttgta ggaaattttg aaaaaatata
      541 tgaaataaac aggtccttta gaaatgaagg gatttcaact ttacataatc ctgaattcac
      601 aatgcttgaa ttatactctg cttacggtga ttatgaagag atgattgaga taacagaggg
      661 attaatctgt tttttatctg aaaaaatttt tggttctctt acttttgaat atcaagggaa
      721 aaagattgaa cttacaagat cgtggaaaag aattaaatat gaagatgttt ttaaagaata
      781 tgcagggatt gagaatttca gagatgaaga agttatagat aaaaaagtga aggaatatca
      841 gatagaagtg gaggaaaatg aaacattatt tgataaactt gaaagtattt ttaaaaagaa
      901 aattcagcca cttcttataa atccaacatt tattattggt tatcctgttg aaatatctcc
      961 acttgcaaaa acatttaaag atagccctga aataacagaa aggtttgaac tttttattgg
     1021 aggtattgag gttgcaaatg cttattctga attaaatgac ccgattgagc agttgaaaag
     1081 atttgaagaa aaatttaaag atgtaaagga taaagagaaa aaaattgatt atgattttgt
     1141 ggaagcactt gaatatggaa tgccaccagc aggaggtctt ggaataggaa ttgaccgact
     1201 tgttatgctt tttaccaatt catcttcaat aagagaagtt atatttttcc ctcttttaaa
     1261 accaaaaaat gagtaaaaaa attttcaggg caatatctct gcttgctatt ggaacaataa
     1321 tttcaaggtt ttttggactt ttcagggaaa ttgtaagtgc taattttttt gggacaacaa
     1381 aaatttatga tgcattttta ctttcattta tgataccgaa ttttttcagg ggacttcttg
     1441 cggaaggtgc tttaacttct tcttttattc ctgtttttac agaatattta tcagatgaag
     1501 gggaaaaaag ccaattaaaa gagattgtgg atataacctt tactttatcg cttatattca
     1561 cattttctct ctttttaatt ttttttatat tgagtgaaat aggagtgaga attctaatat
     1621 atgaaagtaa atggtattat gtttttttgc ttctaaaatt tacctttcca tatctgattt
     1681 ttatttctct ttctgcctta tacacaggaa ttttaaactc ttataaatca ttttttttac
     1741 cttcaatttc acctattgtt cttgattttt tctggatttt atcattattt tattttgctc
     1801 atttttttgg aaatactctt gaagaaaaaa ttttttcatt atgtttagga gttattttag
     1861 gtggaatagg acagtactta atacttgttc catctgtcag aaaacttggt tattctccta
     1921 aaataagttt aaatttttct catccagcaa taaaaaaaat gggtttttta tttcttccga
     1981 tgattgttgg tgttgctgtt ggtcctataa atttacttgt tgattattca cttgcaaatt
     2041 tcttatcatc aggaatggtt tctggactct ggtatgctac acgactttat caacttcctc
     2101 ttggtatatt cggtatttca atttcaacag cagttttacc ttggttgagt gaaaattttt
     2161 caaaaaaaga atttgataag tttagggaaa atctcaatta cgcatttaag attctttttt
     2221 taaccattat accttcaact atctttttga tttttttaaa agacgagata attacatttc
     2281 tctttaaaag aggtatgttt gatgtaaaat cagtaaattt gacctcatat cctctatttt
     2341 tctattctct tggacttgtt ttttacggtg gtatttcagt tatgacaaga tgtttttatg
     2401 cttttaatga tacaagaact cctgtaaaaa tcggtgtttt cagtattgct acaaatttta
     2461 ttcttgatat aattttaatg cagtttcttg ctcatggagg aattgctctt tctacttctc
     2521 ttgtgggact tataaatttt ctcttacttt tttatttttt taataagaaa catttaaaag
     2581 tggattatgg aaagatttta gaaatatttg gagtttcctt tatttctgca attcccatgt
     2641 ttattttttt aacaattttc agaaatttcc ttcttcaaaa acttaattta ttcaattttt
     2701 tatttttatc agtttcttct ggattgattt tatacttctt ttcttttaaa tttataagca
     2761 gaattttaag aaggaggata tgaaatgaaa gggattaaat atgaaggaaa taagattaaa
     2821 cagataaaat ttcttcttgg aggaattggt gcaggtaatg tttctgttga agggaagggc
     2881 tctttaactg actgggaaat atttaataga ccgggaaaag gaaaaagaat gtatgggaat
     2941 tttgtttcat taagggtaaa atcaaaagac gggataaaat atagaattgt ttcaggaaat
     3001 cctttccctc catttgaaag cgctggtggt tatcaaaatc tgaatatgga aggatgcccg
     3061 tgttttgaaa gagttgaatt taccaattat tttcccttcg caattattga tttttcagat
     3121 aaaagattat cattaaaaat tactcttgaa gcatttactc cttttattcc tcttgattat
     3181 gaaaattctt ctctgcctat agcagttttt acatatagaa taaaaaatct caccgaaaaa
     3241 gaacttgaaa tttttcttgc tttttcaatt atgaatcctg tcggaacaga tggaactgaa
     3301 aatttaaatt cacctaaaaa tgtatgtttt ggcgggaata tcaatattta taaagaaata
     3361 ggtgaagtta aaggtcttta tttaaaatca aataaatatg aaaaagaaag tcatagatat
     3421 gggaatattt cattgttcac agattcaaaa aatatttcat ataaaactca gtgggaaaaa
     3481 attggatggt ggactgatta tgaaaatttc tggagagaat ttaaaaacgg caagtttgaa
     3541 tttgttgaaa gtaaagaaag tcaaaatggt gaaacatact gggcaactct cggtgtaaag
     3601 gaaaatttaa aaggaaaaga ggaaaaagag ataaattttt atctttcatg gtatttccca
     3661 aatagaataa attactgggg attgaatgaa gaggtaaaaa ataaaatttt aaaaaactgg
     3721 tattcaaaaa gattttctga ttcagcagaa gtggttaaat actatattaa aaataaaaaa
     3781 taccttaaag atgaaagcat aaaatttgca gaaagttttt tttctcagag tttacccgaa
     3841 agttttcttg caacaataag ttctcagttt aatactttaa gaagcaatac attttttatt
     3901 actgaagatg gacgttttta tgcttttgaa ggatgttctg ataataatgg ttgctgtcca
     3961 ttgaactgta cccatgtcta taattatgat tttactgttt cctatctatt tccgaatttt
     4021 atgaaaagcc agagagaggt ggattatctt tacaatacgg atgaaaacgg ttatatggct
     4081 tttagaacaa atataccttt agggataaaa ctctggcagt ttaaaccagc agcagatgga
     4141 cagatgggaa cagttttaaa agcatacagg gaatggaaaa ttacagggga taatgaattt
     4201 ttgaaaagaa tatatacaaa attaaaaaag tcggttgaat atgcttggga aaactgggat
     4261 aaaaatagag atggtttaat ggaaggagaa cagcataata cctatgatgt tgaattttat
     4321 agagaaaatc catttacttc ttttatttat cttgcatcat taaaagcaat ggaagagatt
     4381 tcggaattta tgggtgatat agattttaaa aatgaatgta aaaggatata tgaagaagga
     4441 aggaaaaagg taatagaaga actctggaat ggaaaatatt ttgttcagaa atgtaaaatc
     4501 aatccattgc caccatacca atttttgaat ggatgtttaa cttctcaatt acttggtcag
     4561 ttttttgcag ataatctaaa tcttggttca attgttgaag aaaagtatat aaaaaagaca
     4621 ttatcttcta ttttaaagta taactttata gatttttcag aacacataaa ttttatgaga
     4681 atatatggaa ttggaaatga aaaaggagtt attgtatgtt ctttcccaga aggtaggcct
     4741 gaaattccaa tgccttactg tgatgaggtc tggacaggag aagaatttgt tattgcttca
     4801 ctgcttatta ggagagggaa attaaaacag gcaaaagaaa ttattgaagc aataaataat
     4861 agatatgatg gtgaaaaaag aaatccattc aatcatattg aatgtggtta tcattatggt
     4921 agaggttttt ctgcttttgg tttaatttta tcctatcttg gtttttttgt tgacaatgtg
     4981 agaaaggaga tttatttttc acctgttttg ctgaaaaaag aattaaatat tttcttttca
     5041 accggaaccg gctggggtaa ttataagtat tctaaaaagg gaaaactaca ggttgttgaa
     5101 atagaaattc tacatggttt tatagaaatg aaaaatttga tttttaagat agataatgtt
     5161 aaaaatagga aaattgaaat taaagtagac gatataaaaa aggattttga ttttgttttg
     5221 aatagaaatt ctgttaaaat attttttggt aaaccagaac taattttaaa aagaataaaa
     5281 gttttaatta aatgaaaaaa aattatatta caaaaaagac atttgaaaaa ataaaagacg
     5341 agaagtttaa tttacttatt ggagcaaatc tcataaccat aaccaatatt cagttgaatt
     5401 ttcataaaat taattctcca acatatcctc actatcatcc agatgctcaa ttactttatt
     5461 atttaaaagg ttcagggata gaggaaattg atgggaaaaa atttaatatt gttcctggtt
     5521 cttttgtctt tattccatct tacaaaaaac atgctttttt cccttataaa gaaacaacag
     5581 cagaaatttt tacaatgaga tttgaagcaa agaagaatat tataaaaaat tcagacagtt
     5641 ctttactttc taagggtttt ttcaaaatac ttaaattatt ttatggtaaa aaaccagatg
     5701 tttggactgt aaaagaagat agaaaaaatg aagttgaaaa cctgataaaa aaaattgatg
     5761 aaaatctctc tgaaaagaaa tttggttata taatttctat tgaaggatat ttacttttat
     5821 taataaatga atttttaact tcctatcttg aaagaagaga aatagtagaa aagaaggcat
     5881 ctaaaaagga gattattttt aagaaagtcg taaatttcat aactgaaaat attgaaaaac
     5941 aggtgacaat aaaagaaatt gcgaaaaatt attacttttc tgtaaactat atccagaaga
     6001 ttataaaaga atttacaggt cgttcattta caagatttat gcatgatatt aaaattgaat
     6061 atgtaaaaga acttttaaaa aatacaacac ttgaaataaa ggaaattgct ggaagatgtg
     6121 gattttatga ccagaattat tttacaagaa tatttaaaaa aattgaaggt attccacctt
     6181 caaagtatag ggaaatttaa tcaatttttg tgctaaatat atttaaattt atcctaatat
     6241 tttttaaaaa taatttttaa aataaaagaa aaggaggata aaatgaaaaa acttgatggt
     6301 ttacttcctg caatagttat accatataaa gagaattatg aagtagatga agaagtttta
     6361 aggaaatatg ttgagtggat aagcaaattt gaaattaaag gtcttgttat aaatagtgat
     6421 gcaggagaag gacatttttt aacccatgaa gaaagaaaga gaaatataaa aataataaag
     6481 gaaataattg gagataaaat tcctcttata agtggacttg gaggacagac aacaaaagat
     6541 gtaattgaat ctggtaaaga atttaaagac cttggagtta attattttct tgtgtttccc
     6601 catccagcat ttaaaggtgg accggcaaaa gataatctaa taataaaata tcatgaagag
     6661 atttcaaaga ttggaattgg aatgattata tttcaactcc aggaagcact tggagggaca
     6721 ttttatcatt ttgaaacact taaagaactt gttgaaattg aaaatgtagt agcaataaaa
     6781 gaagcgacat ttgatgcttt aaaatttaaa gaaactgttg attttttaaa tacactttca
     6841 aaaaagatag aaattttaac aggtaatgac aattttattc cggaatcatt tcttcttgga
     6901 gcagatggtg cattaatagg ttttggttca atttttacag atatacaggt tgatgtaata
     6961 aaaaagatta aggagaaaaa atttgatgag ggtttaaact tattcaggaa aatagaaaaa
     7021 atctgtgtat attgtttcaa aagacctgtc agggattaca gggcaaggat aaaggaattt
     7081 cttgtatgta tgggtatatt taaaaattca cttgtaagaa gtccactttt accattaagt
     7141 aaagaagaaa aagaagaaat aagaaaactt tatgaggaat ttaaaaatgg ataagaagaa
     7201 aaaatttgaa gaattaaaaa aatactatct tgaaaatcca atagatgaga aattctctga
     7261 aattgtaaaa gatgaactcg ggattgactt aaaagcaaaa tatggaaact tcacattaaa
     7321 tcatcctgtt attgttgctc ctggacagtt aacaagagaa aagggacaga tagaaaggat
     7381 aaaaaaagca ggatattccg gatgtgtttt aaaatctgtt gttggagagg atgaaaatgg
     7441 taattgttca atgaaagaat acagaaaaaa aatgacatat cttaaaacag tttatgaaaa
     7501 agatgataaa gaaggtgctt atcctgtaat tttatgggat ggaagggcag ataccagaaa
     7561 tcttacagaa tatatggaat ttgccagaga tgtttataat ttttcagaca gtaaattctt
     7621 gatagttgca tcaattttat gtcatctgcc gaaacctgag gaagaaataa aaaaagaaga
     7681 atggttatat acagtgagaa aactttatga tacaggatac agaatatttg agatagattt
     7741 ctgtccaggg ttaaaaaata acaaaaaaat tattgagaaa gaaaatatat gcaggttata
     7801 cagagaaata ccagaattac ttaaag
//
LOCUS       DAOSAT010000082         7694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_6133_length_7694_cov_4.734389, whole genome shotgun sequence.
ACCESSION   DAOSAT010000082 DAOSAT010000000
VERSION     DAOSAT010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6133_length_7694_cov_4.734389"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1655
                     /locus_tag="PLF90_07320"
     CDS             147..1655
                     /locus_tag="PLF90_07320"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOK57122.1"
                     /translation="MERIYIAIDLGAESGRGIIGNFDENKIYINEIHRFSTYNSIILN
                     HRFWNVLSIFEEIKKILSIAKDKGEIAGIGVTTWGVDCGFIGENNLLLSNPFHYRDER
                     TNGIMEEVFQILPKERIFKETGIQFMPLNTLFQLYATKKEFPYLIDNCKTLLFMPDLF
                     NFFLTGEKFCEYTITTTSQMYNSLASGQNKAPRGNWCYGLLEELGIKTDFLPQVISSG
                     TLIGNITKKLQEELTLPSVPVFAVCCHDTASAVVGTPAKEEEKWAYLSSGTWSLLGVE
                     IDEPIINEKGLEYNFTNEGGFGGTIRFLKNIMGLWILQECKKEWDKTGNIFKYEELVE
                     MAKNATPFYAFIDVNNADFLYPGNMIDKIKNYCKKTGQKIPEDIPQIVRVIFESLAME
                     YRYVIEKMEEILGYEIEILHIVGGGSKNSLLSQFAASSTKKIVITGPSEATSVGNLLI
                     QALARREIKDISQVREIIRNSFPLIVYRPENVEIWDENYQKYLKLKSFHYGL"
     gene            1645..2250
                     /locus_tag="PLF90_07325"
     CDS             1645..2250
                     /locus_tag="PLF90_07325"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOK57123.1"
                     /translation="MDCNFLQNSLKNYFEVLSAKKPVPGGGSAVACLASLSSSLVNMV
                     LNYTIGKKGYEKFQEEIEKIKRKNEEILNECMNFIEEDSRIYEKIDRAIKERLEIEEY
                     LKDSANLHLKICEYMVEIVNFCDILTERGNKNLISDTGIASIFAIGAFFGGKMNILIN
                     LKFLKDENFKIEIKEKIEKMEKEIEEKSKEVKSKVIKIMEE"
     gene            2252..3109
                     /locus_tag="PLF90_07330"
     CDS             2252..3109
                     /locus_tag="PLF90_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007473306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOK57124.1"
                     /translation="MGNIIDGKVIAEKIRDEVKKEIEQLKSKGIIPKLVAVSVGENPA
                     SIVYMNQQKKNCEKIGINYEIMSLSDSIDEKGIIENIEKLNENPDVTGIILQLPLPKG
                     VDTRKVQSKIAPNKDVEGVNPVNMGWIVYGRPVVGPCTALAVKEIIEYLGINLYGKEV
                     VMVGHSDIVGKPVGLLLVDKFATITICHIGTSDAGRLQDHVKRAEILIVAVGKANLIP
                     GDWIKEGAIVIDVGINRVGDKIVGDVEFEKAKEKASWITPVPGGVGPITTAILLRNTV
                     LLVKLQKGV"
     gene            complement(3147..3935)
                     /locus_tag="PLF90_07335"
     CDS             complement(3147..3935)
                     /locus_tag="PLF90_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57125.1"
                     /translation="MKILYEFIPKSNFLELVDEINDLIDGYNITDSASGIPAPSATAV
                     ACIIKTKFPQKIVFPMLITNYKGPVEIGAVAKACESVGIDGMIPDPGDAPRYGFAIRT
                     RKDGNCEIIRDPQEFEQFRRSTGPAEEIRSFLRETIKVKNLQLGCLVTSRRPTEDAIA
                     RIRDQWDFCFFLRLDEASLPKLKELSLECKKLGKPIYPYFVVETPKNKKILERINWPA
                     TTTMENAVEFVKKIEGIVNGIIATCLGDVEGDKELLKILQKVRG"
     gene            4605..5486
                     /locus_tag="PLF90_07340"
     CDS             4605..5486
                     /locus_tag="PLF90_07340"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOK57126.1"
                     /translation="MEISNLQKKLNEGKFVITSEIGPGKGTNLEFLKEAEVIKNMVDA
                     INVTDLQSSVMRLGSLSVCHLLVEMGIEPIFQITCRDRNRLALQSDLLNAYVLGIRNV
                     LVLTGDHPILGDHPEAKSVFDLDSVSLLKVASTLMEGKDMKGNELDGKVEFFIGAVVS
                     PGYEPIDLQIIKMEKKIENGAKFFQTQAVYDLKKFEEFMKRVEKYKVPILGGIVLLKS
                     AGMAKYMNENVAGVTVPDKYIEMMAKAKKEERVKVSLQIGAELIKGMKDMCQGIHIMP
                     LGWDKYVPDLLSLSGII"
     gene            5629..6876
                     /locus_tag="PLF90_07345"
     CDS             5629..6876
                     /locus_tag="PLF90_07345"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOK57127.1"
                     /translation="MGNFNLFEMAQKQLDMVSKILKLDEATHNFLREPMREIHITIPV
                     KMDNGQTRIFKGFRVQYNNARGPFKGGIRFHPEETIDTVRALAAWMTWKTSLLDLPYG
                     GGKGGVICNPKELSDSEKERLARGYIRELAEFIGPEKDIPAPDVYTDPQIMAWMMDEF
                     SKIKGYNSFGVITGKPLALGGSLGRNDATARGGIYCVREAAKYLKINLKNSKVAIQGF
                     GNAGSYAAILSQEILGAKIIAVSDSKGGIYSEKGLDPYKVIEYKKKNGTVTGFPGTSK
                     ITNEELLELDVDVLFPSCLENVISGENSKRIKAKIIAELANGPTTPEADEVLYSKGKF
                     VIPDFLCNAGGVTVSYFEWVQNITGYYWKLEEVHNKLDEKMTKAFYDVIKVYEEKKIS
                     PRMAAYLVAVNRVVESMKLRGWV"
     gene            6882..>7694
                     /locus_tag="PLF90_07350"
     CDS             6882..>7694
                     /locus_tag="PLF90_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOK57128.1"
                     /translation="MKEYCGIVGIFNLKKISEYLYFSLFSLQHRGQESCGIVFSDFND
                     VFIHKGMGLISDVFSRFDFTKYKEKLGFGIGHVRYSTTGNPDIKNIQPFIVEYKGRIY
                     AIAHNGNLVNSYNLRKKLEEEGTLFQTTLDTEIIMHLIFKSKKEKFEDKLKEALSEIK
                     GAFSLVLFTPDGIYAIKDPNGFRPLCLGKLNNGYVISSESCAFDLIGAEYIREIEEGE
                     ILFIGKDGLKSYYLPKNKISRCIFEFIYFARPDSKIFGKSVYVARKKLGENLA"
BASE COUNT         2804 a          931 c         1461 g         2498 t
ORIGIN      
        1 ctgtctttta tcctttggaa caattacctc taaatgtctc cttctccata tatctcttct
       61 ccatttcttg tctacctgga tactatgtct taacccaaaa gcggattttt gttgacataa
      121 aaataattgg atatataata aaatatatgg aaagaattta tatagcaata gaccttgggg
      181 cagaaagtgg aaggggaatt ataggaaatt ttgacgaaaa taaaatttat ataaatgaaa
      241 ttcatagatt ttctacttac aactcaatta ttttaaatca tagattttgg aatgttctta
      301 gtatttttga agaaataaaa aaaatattat ctattgctaa agataaagga gaaattgctg
      361 gaattggagt tacaacctgg ggagtggatt gtggttttat aggtgaaaat aaccttttgc
      421 tttcaaatcc atttcactat agggatgaga gaacaaatgg aataatggaa gaggtttttc
      481 aaattttgcc caaagaaaga atttttaaag aaacagggat tcagtttatg cctcttaata
      541 ctctttttca attatatgcc acgaaaaaag aatttcctta tttaattgac aactgcaaaa
      601 cattactttt tatgcctgat ttatttaatt tctttttaac cggagaaaag ttctgtgagt
      661 atacaatcac aacaacttcg cagatgtata attctcttgc atctggacaa aataaagcac
      721 caagagggaa ctggtgttat ggattacttg aagaactcgg tataaaaaca gattttcttc
      781 cacaggtaat ttcatcagga actttaatcg gaaatataac taaaaaatta caggaagaac
      841 taactttacc ttcagtacct gtttttgctg tttgctgtca tgatactgct tcagcagttg
      901 ttgggacacc ggcaaaggaa gaagaaaaat gggcttatct ttcttctgga acatggtctc
      961 ttcttggagt tgaaattgat gagccaataa taaatgaaaa agggcttgaa tataatttta
     1021 caaatgaagg tggttttggt ggtactataa gatttttaaa aaatataatg ggtttatgga
     1081 tattgcagga atgtaaaaaa gaatgggata aaaccggtaa tatttttaaa tatgaagaac
     1141 ttgttgagat ggctaaaaat gctactcctt tttatgcctt tattgatgtt aataatgctg
     1201 attttcttta cccgggtaat atgatagata aaataaaaaa ttactgtaaa aaaacaggtc
     1261 agaaaattcc agaggatatt cctcaaatag taagagttat ttttgaaagt ttagcaatgg
     1321 aatacagata tgttatagaa aaaatggaag aaatattggg atatgagata gaaatacttc
     1381 atattgtagg cggaggaagt aaaaattcgc ttttatctca atttgctgca tcatctacca
     1441 aaaagatagt tataacagga ccatctgaag caacaagtgt gggaaatctt ttaattcagg
     1501 cacttgcaag aagagaaatt aaagatataa gtcaggttag agaaattata agaaattctt
     1561 ttcctttaat tgtttataga cctgaaaatg ttgaaatatg ggatgaaaat tatcagaaat
     1621 atttaaaatt gaaaagtttt cactatggat tgtaattttc ttcaaaattc gttgaaaaat
     1681 tattttgaag ttttatcagc aaaaaaacct gtccccggag gaggaagtgc agttgcctgt
     1741 cttgcttcac tttcaagttc tcttgttaat atggttttaa attatacaat cggtaaaaaa
     1801 ggatatgaaa aatttcaaga agaaatagaa aagattaaaa ggaagaatga agagatttta
     1861 aatgaatgta tgaattttat tgaagaagat agcaggatat acgaaaaaat tgatagggca
     1921 ataaaggaaa gattagaaat agaagaatat ctgaaagatt ctgctaattt acatcttaaa
     1981 atatgtgaat atatggttga aattgttaat ttctgtgata tacttaccga aagaggtaat
     2041 aaaaatttga tttctgatac aggaattgca agtatttttg caataggtgc tttcttcggt
     2101 ggaaagatga atattctgat aaatctgaaa tttttaaaag atgaaaattt taaaatagaa
     2161 ataaaagaga aaatagaaaa gatggaaaaa gaaatagaag aaaaaagtaa agaggtaaaa
     2221 agtaaagtta taaaaataat ggaggaataa aatgggaaat ataattgatg gaaaggttat
     2281 tgcggagaaa ataagagatg aggtaaaaaa agaaattgaa cagttaaaat caaaagggat
     2341 tattcctaaa cttgttgctg tttctgtagg tgaaaatcct gcaagcattg tatatatgaa
     2401 tcagcagaaa aaaaactgtg aaaaaatagg aattaattat gagataatga gtttatctga
     2461 tagtattgat gagaaaggga ttattgaaaa tattgaaaaa ttaaatgaaa atccagatgt
     2521 aacaggaata attttacaac ttcctcttcc caaaggagtt gatacaagaa aggttcaatc
     2581 aaaaatagca cctaataaag atgttgaagg tgttaatcct gttaatatgg gatggattgt
     2641 atatggaaga ccggttgttg gaccatgtac agcacttgcg gttaaagaaa taattgagta
     2701 tcttggtata aatctgtatg gaaaggaagt tgttatggtt ggtcattcag atatagtagg
     2761 aaaacctgtt ggtttgcttt tggttgataa gtttgctaca attacaatct gtcatatagg
     2821 aacttctgat gcagggagac ttcaagacca tgtaaaaaga gcagagatat taattgttgc
     2881 tgttggaaag gcaaatttaa taccagggga ctggataaaa gaaggagcaa ttgtaataga
     2941 tgtcggaata aatagggtag gggataaaat agtaggagat gttgaatttg aaaaagcaaa
     3001 agaaaaagcg agttggatta cacctgttcc aggaggagtt ggacctatca caactgcaat
     3061 cttattaagg aatactgttt tactggtaaa attacagaag ggagtataaa aatcgtgtta
     3121 tgacgaaaat aaaaacatac aggaatttaa cctctgacct tttgtaaaat cttcaatagt
     3181 tctttatctc cctctacatc gcccagacaa gtagcgatta taccattaac aattccctct
     3241 atttttttaa caaattcaac tgcattttcc atcgtagtag ttgcaggcca atttattctt
     3301 tcaagtatct ttttattttt aggtgtttct acaacaaagt aaggataaat aggttttcct
     3361 aactttttac actcaagaga caattctttt aactttggga gagatgcttc atctaatctt
     3421 aaaaaaaagc agaaatccca ttgatctcta atacgagcaa ttgcatcttc agttggtcta
     3481 cggcttgtca caagacatcc taattgtaaa ttttttactt ttatagtttc tctcaagaaa
     3541 ctccttattt cttccgcagg gcctgtagaa cgcctaaatt gttcaaactc ttgagggtcc
     3601 ctaataatct cacagttgcc atcttttctt gttctaattg caaatccata tcttggcgca
     3661 tctcctggat caggaatcat tccatcaata ccaacagatt cacatgcctt tgccactgca
     3721 ccaatttcaa ctggtccttt gtaatttgta attagcattg gaaacactat tttttgcggg
     3781 aattttgttt tgataatgca agcaactgct gttgctgatg gtgcaggtat tccactggca
     3841 ctatcagtaa tgttgtatcc atctattaag tcattaattt catctactaa ttcaagaaag
     3901 tttgacttcg gaataaattc gtataatatt ttcataaatt tctcctcctt tctaattgtt
     3961 atttttcttt ttttaattta tcaaatcatt ttatctttgt caaatttttt ctctggtttt
     4021 aatatgataa ttataagtgc agaaatatca atgagttaca aagattaatc actatgtcgg
     4081 aattttttag cccttttttc tttttcaaaa taatttatta gtggaagtaa aaattcaatt
     4141 gtgagaaaac tttcaaggta tataagagaa ctttcaaatt cctcatcagt tttagttttt
     4201 cctgtggtat tggtgttcag tgtgtttcaa aagttattga tgtaccagtt tatcctgctt
     4261 caaacacaat ttatcttggt ggatttcagg gactatggcc tggtggggaa agatgtgctg
     4321 aatgtgggac ctgttatctt ggaattacag gaggaatatg ccctattaca ttttgtacaa
     4381 aacaacttat taatggtcca tgtggaggaa caaataaagg aaaatgtgaa atagacccag
     4441 aaaaagattg cggatgggtt ttaatatatg aaagattgaa aaaaatagga aaacttgaaa
     4501 ttttaaaaga ataccaagaa ccaagaaatt ataagttact tgaacctgat tctaaattga
     4561 ggaaaaaggt attttatgat ttagataaaa acgaggagtt ttaaatggaa atttctaact
     4621 tacagaaaaa attgaatgaa gggaaatttg ttattacttc tgaaataggg cctgggaaag
     4681 gaacaaatct tgagttttta aaagaggcag aagttataaa gaatatggtt gatgcaataa
     4741 atgtgactga tttacaaagt tctgttatgc gacttggttc acttagtgtt tgccacttac
     4801 ttgtagaaat ggggatagag ccaattttcc agataacatg tagagatagg aatcgacttg
     4861 cattacaatc agacctttta aatgcttatg ttcttggtat taggaatgtt ttagttttaa
     4921 caggagacca tccaatactt ggggaccatc ctgaagcaaa gtcagttttt gaccttgatt
     4981 ctgtttcatt attaaaagtc gcttcaacat tgatggaagg aaaagatatg aaagggaatg
     5041 aacttgatgg taaagtagaa ttttttattg gagcagttgt aagtcctgga tatgaaccaa
     5101 ttgacctaca gataataaag atggagaaaa aaattgaaaa tggagcaaaa ttcttccaga
     5161 cacaggcagt atatgattta aaaaagtttg aagaatttat gaaaagagtt gaaaaatata
     5221 aggttcctat tttaggtgga attgttcttc ttaaatctgc tggtatggca aaatatatga
     5281 atgagaatgt agcaggagtt acagttcctg ataaatacat agaaatgatg gcaaaggcaa
     5341 agaaggaaga aagagtaaaa gtaagtttac agattggagc agaattgatt aaaggtatga
     5401 aagacatgtg tcaagggatt catataatgc cacttggatg ggataaatat gttcctgatt
     5461 tactttcttt atcaggtata atttgaaaaa taaaattttt ttgacatata attttaatta
     5521 tcgtataatt tataaataga cacaaagtta gtgtaaagga caaagaggtc ctttttccca
     5581 aagtggggga aaggacctct tttttttata aacaaaaagg agggaaaaat gggaaatttt
     5641 aacctttttg aaatggctca aaaacaactt gatatggtaa gcaaaatttt aaaacttgat
     5701 gaagcaactc ataatttttt aagagaacca atgagagaaa ttcatattac aataccggtt
     5761 aagatggata atggccaaac aaggatcttt aaaggtttca gagttcagta taataatgca
     5821 agagggcctt ttaaaggagg gattagattt catccagaag aaacaataga tacagttaga
     5881 gcacttgctg catggatgac atggaagact tctttacttg accttcctta cggaggaggt
     5941 aaaggcgggg ttatatgtaa tcctaaagaa ttatcagatt cagaaaaaga aagattggca
     6001 agaggttata taagggaact tgctgaattc attggtcctg aaaaagatat tcctgctcct
     6061 gatgtttata ctgatcctca aattatggca tggatgatgg atgagttttc aaaaattaaa
     6121 ggttataatt cttttggagt tataacagga aagcctttag cccttggagg ttcgcttgga
     6181 agaaatgatg ctacagcaag aggtggaata tattgtgtaa gagaagcagc aaaatattta
     6241 aaaataaatt taaaaaattc aaaagttgca atacagggtt ttggtaatgc aggttcttat
     6301 gctgctattt tatcacagga aattcttggt gctaaaatta tcgctgtttc tgattcaaaa
     6361 ggcggaattt attctgaaaa agggctggat ccttataaag ttattgaata taaaaagaaa
     6421 aatggaacag ttactggttt tcccggaact tctaaaatta caaatgaaga attgcttgaa
     6481 cttgatgtgg acgttctttt tccttcttgt cttgagaatg ttataagtgg tgaaaattca
     6541 aaaagaatta aggcaaagat tattgctgaa cttgcaaatg gtccaacaac tccagaagca
     6601 gatgaagttt tatacagtaa aggtaaattt gttatacctg attttttatg caatgcaggt
     6661 ggagtaactg tttcctattt tgaatgggtt cagaatataa caggttatta ctggaaactt
     6721 gaagaagtac ataataaact tgatgaaaaa atgacaaaag cattttacga tgttattaaa
     6781 gtttacgaag aaaagaaaat aagtccaaga atggctgcct atctagttgc tgtaaataga
     6841 gtagttgaaa gtatgaagtt gcgcggctgg gtataacttt aatgaaagaa tactgcggaa
     6901 ttgtaggaat attcaatcta aaaaaaattt cagagtattt atatttttct ctattttctc
     6961 ttcaacatag aggccaggaa agttgtggaa ttgttttttc tgattttaat gatgttttta
     7021 tacataaagg tatgggtctt atcagtgatg ttttttcgag atttgatttt acaaaatata
     7081 aagaaaaatt aggttttgga attggacatg ttagatattc aacaactgga aatcctgata
     7141 taaaaaatat acagccattt atagttgaat ataaggggag aatctatgca attgctcata
     7201 atggtaatct tgtgaatagt tataatttaa ggaaaaaact tgaagaagaa ggaacacttt
     7261 ttcaaacaac tcttgataca gaaataatta tgcacttaat ttttaaatca aaaaaagaaa
     7321 aatttgaaga taaattaaag gaagcattat cagaaataaa aggtgccttt tcacttgttt
     7381 tatttactcc tgatgggatt tatgctatta aagacccaaa tggatttagg cctttatgtc
     7441 ttggtaaatt aaataatggt tatgtgatat catctgaaag ttgtgctttt gatttgatag
     7501 gtgctgaata tataagagaa atagaagaag gtgagattct ttttatagga aaagatggtt
     7561 taaagagtta ttatttacca aaaaataaaa tttcaagatg tatatttgaa tttatatatt
     7621 ttgcaaggcc tgacagtaaa atatttggta aaagtgttta tgttgcaaga aaaaaacttg
     7681 gtgaaaatct tgca
//
LOCUS       DAOSAT010000083         7539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_6322_length_7539_cov_5.094735, whole genome shotgun sequence.
ACCESSION   DAOSAT010000083 DAOSAT010000000
VERSION     DAOSAT010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6322_length_7539_cov_5.094735"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..767)
                     /locus_tag="PLF90_07355"
     CDS             complement(15..767)
                     /locus_tag="PLF90_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57129.1"
                     /translation="MKEKIYLWAQKEEMQNNRWGLPEKSKISPVDACKYLGIENIIFV
                     REFGKPEPSEYEKYAESFKELKKVVWSVVGAGCSYEEGEIERVIELKKKYKNITGAVM
                     DDFFVKERPPFTPEEITGIKERLNENGLELWTVLYQHQLDLQVKEYLERFDLITYWIW
                     WSEGINELEKSFDKFCKIVPSKKKALGIYMWDYGNNKGMPLDLFQKECEIGLKWLKEN
                     KIEGIIFLASCIVDLGLPTVEWVKNGLLLYRY"
     gene            complement(764..2020)
                     /locus_tag="PLF90_07360"
     CDS             complement(764..2020)
                     /locus_tag="PLF90_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57130.1"
                     /translation="MGYNRIKQLLTDPVREELIKEEKISFEKIINKFPLLKGEPAFDP
                     ELIKVNIKKLKTIPEIDTGIPCLDKAIKIGLAHIDVTFQGDHPKYGVKSYAQNRHDGF
                     PPTIISAVDALTLWGINDRAKQIFKYYILNFIKEDGTINYYGPSISEYGQLLNISYLL
                     LERAGEENWWKDCFPKLNKLAFYLYGLWEEAQKEDGLIEGGPEADEIELYNRTGKYFH
                     NNAWASNGFLKWAEVCEKTGYISETLILNFKTAGEKLKKDTLNSIKDIWPSDKKDYRL
                     SSQIEPLKKTKYITETVEGSYTNYRYWLELLSSGILPENLANKVVEARLNFGGQFCGM
                     TRFSNHLDDWPLTDYLYGLWNLGRKNDFLISLYGHIFYHQAEEHLTAYEQVSFPPGKE
                     LAPYCLPCQLVAARAGRLLNKKQDAK"
     gene            complement(2141..2386)
                     /locus_tag="PLF90_07365"
     CDS             complement(2141..2386)
                     /locus_tag="PLF90_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57131.1"
                     /translation="MQNNRWGLPEKSKISPVDACKYLGIENIIFVREFGKPEPSEYEK
                     YAESFKELKKVVWSVVGAVYFFSRMTLLNDFRNRWRI"
     gene            complement(2429..3340)
                     /locus_tag="PLF90_07370"
     CDS             complement(2429..3340)
                     /locus_tag="PLF90_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOK57132.1"
                     /translation="MENNKIRWEMGVGLGYNGNKSEAKRKLDYIVELGYSVILASLFN
                     EKERRLLTGEEIEEFSEILKEYSVEVYTTHAIWLTEEPNKPISEILPFQESIIEKAFI
                     LGAKCLTYHFGLCKGLQEGEDFAFGKCLNRYKVSLEDFRKLSIYALKETCRKAKKYGI
                     TVTIENLPAGCLCDLGTTVKDLLEIIKEVDEQNLGICFDSGHAFISGLNLFKELSKIG
                     GYLFETHFHDNIGRISDQNIINDLHQPVGIGIINWIEVITGLKTINFSKPVVFEISSD
                     ENTLTVNKLNWERFLKIYEEKFSKWEF"
     gene            complement(3352..6756)
                     /locus_tag="PLF90_07375"
     CDS             complement(3352..6756)
                     /locus_tag="PLF90_07375"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOK57133.1"
                     /translation="MYSYGKKMIFNLLIIVLFSTFPLKADIVIEKNTPVSIIISENAS
                     ETEYFAATELQKYINLINGISIPVIKDAKVDITSVRIYIGKSKYTEQYLSQFDRSVTG
                     PGQDSFFIKARDGQIVLVGGGDRGTLYSVYEFLEQQGCRWFMPGKLGEVIPRKEKIVI
                     KEGEKLYVPNFIQREITIGDGDNISVEEIIDWCAKNKINRNFNIGSRRKEYYQFWEKR
                     GGDLKWQHICHNYIFILPNEKYFKEHPEYYALYKGQRVELGNEKGNVCTTNPDVIRIF
                     TDFVIDWFDKNPDGAVFPISPPDGLIRWCECPECQKLGGINFMPGEKGSMSRRQIVFL
                     NEIAKRVYEKHPDRYLLLLAYQNTVDPVPDLKLEKNVIVQVAHHGCLVHGIDKCEENK
                     KMKERFEGWAKIAHPLSGSPGIWDYFLLQIDGMSGSPYAPLPFATTAKDNIRYLKKLG
                     GKYYFTQAGPVQIYNPLPFYVIAKLTWDTKMDFEKLCDDFFEKFYGESCKPMKGYWML
                     LENAVQKSDWHCTKWQDLTLPSPKVFTPEVLQKAEKYLEEAERIAKNEIIKQRIGLVR
                     KSFEYVKSIVGQKKEWRLKREKDYYEINTEGTPEDEDFLKKLIVQGEQYGDPDGSIKR
                     MIFRIKKRKEPVINLENERILVSVIPGIGGRIIRLVDKKTGINYMREPLEENYLSDIG
                     SFYFDYGGYEEYIGKQFASPGWEEEFQYSIVENDRQKRIILTTDIQDFNLERTISIEK
                     GTSPEISIQSKLTYKGTSSKKTKLRIHPEMEIKKGNTENIYIWYQTKEGIFRRINLSN
                     ILTEQPYDTWAAIDEKENIGIVNYFEPENVECYLFYNPEKGFFNMELMSFEKELKNGE
                     SIIISHRYKLLEDAKKEFLKQESISDNLDNTLIEVEKLSGTIKFGEGIVNKSAVFEGK
                     EYLHYDAKNTIKGNAGTIEMWIKPYKDAEEIEFEPLISIGNNAPSWFLISLNKGTLGF
                     LYKKGQKPFQKEEEFYSNLSVNIKNWKKMEWHHIGIVWANLERAKSIVQIYIDGELKE
                     SRYNMNIGEDLEGDVLGIGCNTAGMLSPRFCGEIDELRISNHPKTKKEIFESYEQGKR
                     KEPLTADDGTLLLLNFDGKITTGISKSIEKISKEKIRFYVEELLKD"
     gene            complement(6777..7463)
                     /locus_tag="PLF90_07380"
     CDS             complement(6777..7463)
                     /locus_tag="PLF90_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOK57134.1"
                     /translation="MERRKRKKRRSGFTLIELLVVVAIIAILAAMLLPALSQARERAR
                     QAVCMNNLKQLGLATAMYIEDYDQRIPFAYVQGGSYSGYADARFGTWFALLAPYVNMQ
                     VQTWPTSLKIKRAHPFDCPSRRGLGISYGPSICTAQDSSGGVRIIPFENGISNLKYQR
                     VKNPSYKVWLIDTNSNSVFCFNPYHSSSGWCDFIHNIGANILFFDWHVEWKNANDIER
                     MRTYYYRPAF"
BASE COUNT         2249 a         1533 c          861 g         2896 t
ORIGIN      
        1 cagttattgt cccatcaata tctataaaga agtaatccat ttttaaccca ctcaacagtt
       61 ggaagtccaa ggtcaactat acaacttgca agaaatatta taccttctat tttattttct
      121 tttaaccact ttaatcctat ttcgcattct ttttgaaata aatcaagagg catcccttta
      181 ttgtttccat agtcccacat atatatacca agtgcctttt ttttacttgg aacaatctta
      241 caaaatttat caaaagattt ctcaagttca tttatccctt cagaccacca tatccagtaa
      301 gttattaagt caaacctttc aagatactct tttacctgta aatctaactg atgttgatat
      361 aaaacagtcc atagttcaag tccattttca tttaatctct cttttattcc tgtaatttcc
      421 tcaggtgtaa aaggtggtct ttctttcaca aaaaagtcat ccattacagc accggttata
      481 tttttatatt ttttcttcag ttcaattacc ctctctattt ctccttcttc ataactacat
      541 cctgctccca caacactcca tacaactttt tttaattctt taaaactttc tgcatatttt
      601 tcatactcag atggctctgg ttttccaaat tctcttacaa atattatgtt ttctatgcca
      661 agatatttgc acgcatcaac aggagaaatc tttgattttt ccggtaaacc ccacctatta
      721 ttctgcatct cttctttttg cgcccacaaa tatatttttt ctttcatttt gcatcctgtt
      781 ttttattcaa taatctacct gctcttgctg caacaagttg acatggtaaa caatatggtg
      841 caagttcttt ccctggtgga aaactcacct gttcataagc agttagatgt tcttctgctt
      901 gatgataaaa aatatggcca taaagagaaa tcaaaaagtc attctttcta cctaaattcc
      961 ataaaccata taaataatca gttaaaggcc agtcatcaag atgatttgaa aatcttgtca
     1021 ttccacagaa ttggcctcca aaatttaatc ttgcttcaac tactttattt gctaaatttt
     1081 ctggtaaaat cccacttgat aatagttcta gccagtatct gtagtttgta taagaccctt
     1141 ctactgtttc tgttatatat tttgtttttt ttaatggttc aatctgggaa gataacctat
     1201 aatctttttt atcagaaggc cagatatctt taattgaatt taatgtatct tttttcagtt
     1261 tttctcctgc agttttaaaa ttcaaaatca atgtttctga aatgtaccct gttttctcac
     1321 atacctctgc ccactttaaa aatccattgg atgcccatgc attattatgg aaatatttac
     1381 ctgttctatt ataaagttca atttcatctg cttcaggtcc tccttcaatt aatccatctt
     1441 ctttctgcgc ttcttcccat aatccatata aataaaaagc aagtttattt aattttggaa
     1501 aacaatcctt ccaccagttt tcttcccccg ctctttcaag aagtaagtaa gatatattaa
     1561 gcaactgacc atattcactt attgaaggcc catagtaatt tatagtccca tcttctttta
     1621 taaaatttaa aatataatac ttgaaaatct gttttgctct atcattaatt ccccataaag
     1681 ttaaagcatc aactgctgaa attattgttg gtggaaatcc atcatgtcta ttttgtgcat
     1741 aactttttac tccatatttt ggatggtctc cctgaaaagt aacatcaata tgggcaagtc
     1801 ctattttaat tgctttatct aaacaaggaa tacctgtatc aatttcagga atagttttca
     1861 atttttttat attaacttta attagttcag ggtcaaaagc aggttctcct tttaaaagag
     1921 ggaatttatt aattattttt tcaaaagaaa ttttttcttc ttttataagt tcttccctta
     1981 cagggtctgt caataactgt ttaattctat tatatcccat tctcttaccc atttctgacg
     2041 taaatattga aactcattgt tttaatttta cctttttatt ttccaacttt cttttatgtt
     2101 ataccacact ttcattatac cccccaaaga ttttgtcagg tcaaattcgc catctgtttc
     2161 tgaaatcgtt gagtagagtc atccttgaaa aaaaatatac tgctcccaca acactccata
     2221 caactttttt taattcttta aaactttctg catatttttc atactcagat ggctctggtt
     2281 ttccaaattc tcttacaaat attatgtttt ctatgccaag atatttgcac gcatcaacag
     2341 gagaaatctt tgatttttcc ggtaaacccc acctattatt ctgcatctct tctttttgcg
     2401 cccacaaata tattttttct ttaatatttt aaaactccca ttttgaaaat ttctcttcat
     2461 aaatttttaa aaatctttcc cagttaagtt tattcacagt aagtgtgttt tcatcagaac
     2521 ttatctcaaa aactaccggt tttgaaaaat ttatcgtttt taaacctgtt attacttcta
     2581 tccagttaat tattccaata ccaactggct gatgaaggtc atttataata ttctggtctg
     2641 agattcttcc aatattatca tgaaaatgtg tctcaaaaag gtaccccccg atttttgata
     2701 attctttaaa taaattcaac cctgaaataa aagcatgacc ggagtcaaaa catatcccaa
     2761 ggttttgttc atcaacttct tttataattt caagaaggtc tttaactgtt gtccctaaat
     2821 cacaaagaca tccagcaggt aaattctcaa ttgttacagt tattccatat ttttttgcct
     2881 tcctgcaagt ttctttcaaa gcataaatac ttaattttct gaaatcttct aaagaaactt
     2941 tatatctatt taaacacttc ccaaaagcaa aatcttcccc ttcctgcaaa cctttgcata
     3001 aaccaaagtg ataggttaaa cattttgcac ctaaaataaa cgctttctct ataatgcttt
     3061 cttgaaaagg caaaatttca gaaataggtt tatttggttc ttctgtaagc catattgcat
     3121 gggttgtata tacttctaca gaatattctt tcagaatttc agaaaattct tcaatttctt
     3181 cacctgttaa aagccgtctt tctttttcat taaataaact cgctaaaatt acagaataac
     3241 ctaattcaac aatataatct aattttctct ttgcttctga tttatttcca ttatatccaa
     3301 ggcccactcc catttcccat cgaattttat tattttccat tttaatattt tttaatcttt
     3361 caataattct tctacataaa atcttatttt ttccttagaa attttctcaa tgcttttaga
     3421 aataccagtg gttatttttc catcaaaatt gagaagtaac agtgtcccat catctgcagt
     3481 taaaggttct tttcttttac cttgttcata actttcaaaa atttcttttt ttgtttttgg
     3541 atgattagat atccttaatt catctatttc tccacaaaaa cgagggctca acattccagc
     3601 ggtattacat cctattccta aaacatctcc ttctaaatcc tctcctatat tcatattata
     3661 tctgctctcc ttcaactctc catctatata tatttgtaca atgctttttg ctctttcgag
     3721 attagcccag acaattccta tatgatgcca ttccatcttt ttccagtttt ttatattcac
     3781 agataaattt gaataaaact cttcttcttt ctgaaatggc ttttgtcctt ttttgtaaag
     3841 aaaccctaat gttcctttgt ttaaagaaat taaaaaccag gaaggggcat tattcccgat
     3901 actgattaat ggttcaaatt caatttcttc agcatcttta tatggtttta tccacatttc
     3961 aattgttcct gcatttcctt taattgtatt ttttgcatca taatgtaaat attccttacc
     4021 ttcaaaaact gcagatttat tcactattcc ttccccaaat tttatagttc ctgaaagttt
     4081 ttccacttct ataagggtgt tatccagatt atccgaaata gattcctgtt ttaaaaactc
     4141 ctttttagca tcttcaagta atttataacg atggcttata ataatactct ctccattttt
     4201 caattctttt tcaaaactca taagttccat attgaaaaaa cctttttcag ggttgtaaaa
     4261 taaataacat tctacatttt caggttcaaa ataattaact ataccaatat tctctttctc
     4321 atcaattgca gcccaggtat cataaggctg ttccgtaagg atattagaaa gattaattct
     4381 tctaaatatc ccttccttag tttggtacca aatataaatg ttttctgtat ttcctttttt
     4441 aatttccatt tcgggatgaa tccttaattt agtttttttt gaagaagttc ctttataagt
     4501 aagttttgac tgaatagata tttctggaga ggtacctttt tcaatactga tagttctttc
     4561 taagttaaaa tcctgaatat ctgttgttaa tatgattctt ttttgtctat cattttctac
     4621 aatggaatat tgaaattctt cttcccagcc cggtgatgca aattgtttac ctatgtattc
     4681 ttcataacca ccataatcaa aataaaaaga acctatatca gaaagataat tttcttctaa
     4741 tggctctctc atataattta tacctgtttt tttgtcaact aatcttatta ttcttccacc
     4801 aattccagga ataacagaaa ccaaaattct ttcattttct aagttgataa caggttcctt
     4861 tctcttttta attctgaaaa tcattctttt tatagaacca tcagggtcac catattgttc
     4921 tccttgaaca atcaattttt ttaaaaaatc ttcatcttcg ggcgttcctt ctgtatttat
     4981 ctcataataa tccttttccc tttttaatct ccattccttt ttttgtccta caatagattt
     5041 aacatattca aaagatttac ggacaagtcc tattctctgt tttataatct cattctttgc
     5101 aattctttct gcttcttcaa gatatttttc tgccttctgc aaaacttctg gagtaaaaac
     5161 ttttggagag ggaagagtta aatcctgcca cttagtacaa tgccaatcag atttttgaac
     5221 agcattttca agcaacatcc agtatccttt cataggtttg caagattcac cataaaattt
     5281 ttcaaagaaa tcatcacata gcttttcaaa atccattttt gtatcccatg ttaattttgc
     5341 tattacataa aacggcaaag gattataaat ttgaactgga cctgcttgag taaaataata
     5401 tttaccacct aactttttta ggtatctaat attatctttt gcagttgttg caaatggaag
     5461 tggtgcgtat ggagagccag acattccatc tatctgtaaa agaaaataat cccatatgcc
     5521 cggacttcct gataaaggat gagcaatttt tgcccagccc tcaaatcttt ctttcatttt
     5581 tttgttttct tcacatttat ctattccatg aacaagacat ccatgatgcg caacctgaac
     5641 aattacattt ttttccaatt tcaaatcagg cacagggtca acagtatttt gataagcaag
     5701 aaggagtaag tatctgtcag gatgtttttc ataaactctt tttgctatct catttaaaaa
     5761 tacaatttgc cttctgctca tggaaccttt ttcccctggc ataaaattta tcccccctaa
     5821 tttctgacat tcaggacatt cacaccatct tattaaacca tcaggaggag agattgggaa
     5881 aactgctcca tctggatttt tatcaaacca gtcaatcaca aaatcagtaa aaattcttat
     5941 tacgtcagga tttgtagtgc aaacattgcc tttctcattt ccaagttcta ctctttgtcc
     6001 tttatataaa gcataatatt ctggatgttc tttaaaatat ttctcatttg gaagtataaa
     6061 aatataatta tggcatatat gttgccattt taaatcccca cctctttttt cccagaattg
     6121 ataatattct tttcttctac ttcctatatt aaaattcctg ttaattttat tttttgcaca
     6181 ccaatcaatt atttcttcaa cacttatatt atctccatcc cctatagtaa tctctctttg
     6241 aataaaattc ggtacatata acttctctcc ttctttaatg actatttttt ctttccgggg
     6301 aataacttct ccaagttttc caggcataaa ccatctacaa ccctgctgtt caagaaattc
     6361 ataaacacta tacaaagtcc ccctatcccc tccaccaaca agaactatct gaccatctct
     6421 ggctttaata aaaaaactat cctgtcctgg tccggtaacg gatctgtcaa attgggaaag
     6481 gtactgttcc gtatatttac ttttgcctat ataaattcta accgatgtga tatctacttt
     6541 tgcatctttt attacaggaa tacttatccc atttataaga ttaatgtatt tttgtaactc
     6601 tgtagcagca aaatattctg tttcactcgc attctcagaa ataatgatgg aaacaggagt
     6661 gtttttttct ataactatat ccgccttcaa tggaaaagtg gaaaataata ctattattaa
     6721 taaattaaaa atcatcttct ttccataact atacatcacc cctcctcgtt cttacttcaa
     6781 aacgctggtc tgtaataata agtcctcatt ctttctatat catttgcatt tttccattcc
     6841 acatgccagt caaaaaataa tatatttgcg cctatattat gtataaaatc acaccatcca
     6901 gaagaagagt ggtaaggatt aaagcaaaaa acagagtttg agtttgtatc aattagccaa
     6961 actttatagg aagggttttt aactctttga tatttaagat tgcttattcc attttcaaaa
     7021 ggaataatac gtacaccccc tgaagaatcc tgagcagtgc agatgcttgg accataactt
     7081 attcctaatc ctcttcggct tggacaatca aaaggatgtg ctcttttaat ttttaaagag
     7141 gtaggccatg tctgaacctg catatttaca taaggtgcaa gtaaagcaaa ccatgtacca
     7201 aatcttgcat ctgcataccc actatagcta cctccctgta cataagcaaa cggaattctt
     7261 tggtcataat cttcaatata catagcagtt gccagtccta actgtttcaa attattcata
     7321 catactgcct gacgtgctct ctctctcgcc tgtgatagtg ctggcaataa catcgctgct
     7381 aaaatcgcta tgattgccac cactaccagc aactctatca atgtaaaacc actccttctt
     7441 ttctttcttt ttctccgttc cattttctcc tcctttaccc ctcactcttt cttatctttc
     7501 tttctatgtg aggggatttg actttctctt tcctttcgc
//
LOCUS       DAOSAT010000084         7515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_6348_length_7515_cov_5.270375, whole genome shotgun sequence.
ACCESSION   DAOSAT010000084 DAOSAT010000000
VERSION     DAOSAT010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7515
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6348_length_7515_cov_5.270375"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="PLF90_07385"
     CDS             <1..994
                     /locus_tag="PLF90_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK57135.1"
                     /translation="YIIYLSGYYEFTLETLKKLREKSILIGWFFDDEFRFENYTKYYL
                     PYFDFFVTHSIEAIEKYENFNVNVIHVLPSDGVPFYPDWDKKEEIYDVSFIGFKTEDR
                     ERYINYLKNLNLNIFLAGYGWEKYLTFNEMNEVFRKSKINLCFTKTFNNKRQWKGRIF
                     EVMLQGGFMLTEYVTYLEKYFEIGKEIDCFDNEKELVDKVKYYLKNEKKRKEIAKNGW
                     KKCIENYTPYHRMLNVFLEIEKKVNLERKQFTDYEIFKRRLKKNYSDYYIYMCKAFIL
                     SGHKRYGWETLILAFKNNFLNYKIYLYCLLTLIPFYFFKFLLKFYKNLKKTIKK"
     gene            991..2430
                     /locus_tag="PLF90_07390"
     CDS             991..2430
                     /locus_tag="PLF90_07390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOK57136.1"
                     /translation="MKKLLLIFLFFLPVLLYHINIPILDTYSFRQTQTAAVTRNFYKN
                     GINFLKTELDVFGTGNEKYLLLEFPVYQAIVCIFYKLFFPSEIWGRIVSIIMAYLGAF
                     LLFKLLFLLTDDLNFSLLTTFIYLSIPVNIHFNRTFLMEPTVIATILGFLYFFTLGIL
                     KENIYFWILGIIFSSISFLHKSLYGPFFLLPVVYLIIFKNKKFNKFILLLSLLIPLSL
                     MFRWQMYCDEMNLMNNHFSLTLADKAYRIRNFGTISERFIFETYKQGILNLFPELLTH
                     LTLIPVIFGIFTIKRRKNYQYFYFLFLSSFLFYFVLIKIQCHHYYNMLITPIVSIFCA
                     EGLLYFKNLFKSEHSKKLFLTLFLIVNTLISLKFAYRYHKFDAPLALKIGEIVKKETK
                     PDENIIICFPGYDWNSAYTYYAERKGIHIESSQLKENTIKNLYRKNYKLLILMEWQNY
                     LKFDEKQKFLYNFDVIEKNDDFIILKLKW"
     gene            2424..3404
                     /locus_tag="PLF90_07395"
     CDS             2424..3404
                     /locus_tag="PLF90_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HOK57137.1"
                     /translation="MVITRTPFRISFFGGGTDYPSWYRYNKGAVLTTSIDKYCYITCR
                     YLPPFFEHKHRIVYSIIETVKEIDEIKHPAVRGVLKYLNIDFGVEIHHDGDLPARTGL
                     GSSSSFTVGLLNALYALLGKMKSKEELAKEAIYVEQKILKENVGCQDQIEVAFGGLNK
                     ITFLPDDSFIVEPLILPPGKKEDFEKHLLLYFTGISRYASEIASEQIKNIENKKYELK
                     ILHEMVEEGIKILVGNSEIKEFGKLLDEAWRFKRSLSDKVSNPFIDEIYEKAKKAGAI
                     GGKLLGAGGGGFMLLFAPPELHQKIKEEIKGLFVPFKFENQGSQIIFYQP"
     gene            3415..3564
                     /locus_tag="PLF90_07400"
     CDS             3415..3564
                     /locus_tag="PLF90_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOK57138.1"
                     /translation="MEIFKELLKFLWERKKFWLIPVVIILLLIGILIFVSSITGVSPF
                     IYPLF"
     gene            3924..5759
                     /locus_tag="PLF90_07405"
     CDS             3924..5759
                     /locus_tag="PLF90_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HOK57139.1"
                     /translation="MKPIYILGISAFYHDSASCLLEDDKIISAVQEERFTRTKHDPSF
                     PINSIKFCLDYANLTPDKLDYIVFYEKPFIKFERLLETHFLHAPKSLIQFVQSMPLWL
                     KERILIKNIIKKEIEEKLNEKLSPNTKILFCPHHLSHASSAFFPSLFEKASILTIDGV
                     GEWATATLGFGNKNKINFFYELKFPSSVGLLYSSFTYYTGFKVNDGEYKLMGLAPYGN
                     PHSEKTKRYIKTIKEKLIEIKEDGSIKLNMDYFAFNTSFKMVNEKKWIETFGIPPRKP
                     ESKILTEYADMAYAIQNVLEEILIKMAKFLKEITKEKYLVIGGGVGLNCVANSKLLKE
                     KIFEDIWVQPAATDAGGAVGAAYAVYHMYLGKERICNGKDKMEGCFLGPEYNSYEIEK
                     TLKKYDNLQFYKEENQDKLCNLIAKLINEKKIIGWFSGRMEFGPRALGARSILADARD
                     PEMQKKLNLKIKFREGFRPFAPSVLWEDTQEYFEWDRPSPYMLFTAPVKENRRKNIPE
                     DYYEWDIKDKLYFIRSDVPAITHIDFSARLQTVHKETNYKFWNLIKTFKELTGYSIII
                     NTSFNVRGEPIVCTPEDAIKCFLKTEMDCLVMEDFIIFKKDKKEK"
     gene            5759..7003
                     /locus_tag="PLF90_07410"
     CDS             5759..7003
                     /locus_tag="PLF90_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57140.1"
                     /translation="MKRKKEISIYHIMTVLIILTFVFSFFIFYILTKVCINLSLRITG
                     ASIIWEGSSFKIIQRTERHYRNFIEKMIFCFSDRYTQLFLPQISKLPRLINHPIIQKE
                     MPHSERAFFKKPKNEYVRHATMVYTPYVHYAPAPGYMGGVFHNNQQFRYPENLDKKKI
                     NEIRIFITGGSAAWGCLMPDNKSTISAFLEEEFKKFPDKNYFFRVINAACGGYHTTDE
                     RIWIFNRITEFEPDVIISYSGYNDIYNVYRVKQDLFSALHNEGSYFYWAYREYEYYNN
                     RDFILKITPDFFHFFYTPNDFPRKTIKNIHIISSYLNSIGVKYIFVLQPFRSDNPEEC
                     LIMAEKLEKELKQVSKLFNFKFISYRNFFLNQEEYFIDLCHLGDKGNKVIACNLYREL
                     IPVIYEIIKNKKGVSNEKKQIY"
     gene            6981..>7515
                     /locus_tag="PLF90_07415"
     CDS             6981..>7515
                     /locus_tag="PLF90_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOK57141.1"
                     /translation="MKKNKFIEYEKKAKDLRKLVLDMCVKAGTGHVTSSFSCIEILVA
                     LYYGGILKYKVDNPKWEKRDRFILSKGQASPILYVILADLGFFPISACDKFCCADGMF
                     GVHLQNDVPGVEITTGSLGHGLGIGAGMAYIAKKEKRDWFVFVLLGDGELYEGSIWES
                     ALFVSHHKLNNLVAIVDR"
BASE COUNT         2820 a          866 c         1138 g         2691 t
ORIGIN      
        1 ttatattatt tatctttcag gatattatga gtttacatta gagacattaa aaaaattaag
       61 agaaaaatct attttaattg gatggttttt tgatgatgaa ttcagatttg aaaattatac
      121 aaaatattat cttccttact ttgatttttt tgtaacccat tcaattgaag caatagaaaa
      181 atacgaaaat tttaatgtaa atgttattca tgttctacct agtgatggag tcccttttta
      241 tcctgactgg gataaaaaag aagaaatata tgatgtatca tttattgggt ttaaaacaga
      301 agatagagaa agatatataa attatttaaa aaatctaaat ttgaatattt ttcttgccgg
      361 atatggatgg gagaaatatt taacttttaa tgaaatgaat gaggttttca gaaaaagcaa
      421 aataaattta tgctttacaa aaacatttaa taacaagaga caatggaaag gaagaatttt
      481 tgaagttatg ctacagggtg gttttatgtt aactgaatat gttacatatc ttgaaaaata
      541 ctttgaaata ggaaaagaaa tagattgttt tgataacgaa aaagaacttg ttgataaagt
      601 aaaatattac ctgaaaaatg aaaaaaaaag aaaagaaata gcaaaaaatg gatggaaaaa
      661 atgtattgaa aattacactc cctatcatag aatgttaaat gtttttttag aaatagaaaa
      721 aaaagttaat cttgaaagaa aacaatttac cgattatgaa atatttaaaa gacggttgaa
      781 aaaaaattat tctgattatt acatttatat gtgtaaagca tttattctat cgggtcataa
      841 aagatatgga tgggaaactc ttatacttgc ttttaaaaac aattttttaa actataaaat
      901 atatctttac tgtcttttaa cattgattcc tttttacttt tttaaatttc tgttaaaatt
      961 ttacaaaaac ttaaaaaaaa ctattaaaaa atgaaaaaat tattattaat ttttcttttc
     1021 tttcttcccg ttcttcttta tcatataaat attccaattc ttgatacata ttctttcaga
     1081 cagacacaaa cagcagcagt aacaagaaat ttttataaaa atggaataaa ttttttaaaa
     1141 actgaacttg atgttttcgg gacaggtaat gaaaaatatt tacttcttga atttcctgtt
     1201 tatcaggcaa ttgtatgtat tttttataaa ttattttttc catcagaaat atggggaaga
     1261 attgtttcaa taataatggc ttatttaggt gcttttttac tttttaaact tcttttttta
     1321 ttaacagatg atttaaattt ttctttactt acaactttta tttatctttc catacctgtt
     1381 aatatacatt tcaacagaac ttttttgatg gaaccaacag ttattgcaac tattcttgga
     1441 tttctttatt ttttcactct tggaatttta aaagaaaaca tatatttctg gattcttgga
     1501 attatatttt catcaatctc ttttctccat aaatcacttt atggtccttt tttccttctt
     1561 cctgtagttt atttaataat ttttaaaaat aaaaaattca ataaatttat tttgctttta
     1621 tcccttttaa tacctctttc ccttatgttt agatggcaaa tgtattgtga tgaaatgaac
     1681 ttaatgaata atcacttttc tctaacactt gcagataaag catacagaat aagaaatttt
     1741 gggactattt ctgaaagatt tatttttgaa acatacaaac agggaatttt aaatctattt
     1801 cctgaacttc taactcattt aaccctaata cctgttatat ttgggatttt tacaattaaa
     1861 agaagaaaaa actatcaata tttttatttt ctttttcttt cctcatttct tttttatttt
     1921 gtccttataa aaattcaatg tcatcactat tacaatatgc taattactcc tattgtttct
     1981 attttttgtg ctgaaggact tttatatttt aagaatttat ttaaatcaga acattcaaaa
     2041 aaattatttt taacactctt tttaatagtt aataccttaa tttcacttaa atttgcttac
     2101 agataccata aatttgacgc tcctcttgct ttaaaaatag gagaaattgt aaaaaaagaa
     2161 acaaaacctg atgaaaatat aattatttgt ttcccgggat atgactggaa ttccgcttat
     2221 acatattatg ctgaaaggaa aggaatacat atagaaagca gtcaattaaa ggaaaacaca
     2281 ataaaaaatc tgtacagaaa aaattataaa cttctcattt taatggaatg gcagaattat
     2341 ttgaaatttg atgaaaaaca gaaatttttg tataattttg atgtaattga aaagaatgat
     2401 gattttataa ttttaaaact aaaatggtaa taacaagaac tccttttaga atttcttttt
     2461 ttggaggagg gactgattat ccttcttggt atagatataa taaaggtgct gttttaacaa
     2521 cttctattga taaatactgc tatattacat gcagatactt acccccattt tttgaacata
     2581 aacacaggat tgtatattcc attattgaaa ctgttaaaga aattgatgaa attaagcatc
     2641 cagcagtaag gggagtgtta aaatatttaa acattgattt tggagttgaa attcaccatg
     2701 acggagacct tccagcaaga acaggacttg gctcaagttc ttcatttaca gttggacttt
     2761 taaatgcctt atatgcactt cttggtaaga tgaaatcaaa agaagaactt gcaaaagaag
     2821 caatatatgt ggaacagaaa attttaaaag aaaatgttgg ctgtcaggac cagattgaag
     2881 ttgcatttgg tggattaaat aaaattactt ttttaccaga tgatagtttt attgttgagc
     2941 cattaattct tccaccggga aaaaaagagg actttgaaaa acacctgctt ctatatttta
     3001 ccggaatttc aagatatgct tctgaaattg caagtgaaca gattaaaaat attgaaaata
     3061 aaaaatatga actaaaaata ctacatgaaa tggttgaaga aggaataaaa atacttgttg
     3121 gaaatagtga aattaaggaa tttggaaaac ttcttgatga ggcgtggaga tttaaaaggt
     3181 ctctttctga taaagtttca aatccattta ttgatgaaat ttatgagaaa gcaaaaaaag
     3241 caggtgcaat aggaggaaaa cttcttggag caggtggagg tggatttatg cttttatttg
     3301 ctcctcctga gttacaccaa aaaataaagg aagaaataaa aggacttttt gttccattta
     3361 aatttgaaaa tcaaggcagt cagattattt tctaccaacc ctaaaggagt ttaaatggaa
     3421 atttttaaag aattattaaa atttttatgg gaaagaaaaa aattctggct aattcctgtt
     3481 gttattatac ttttacttat aggaatttta atatttgtta gcagtataac aggggtttct
     3541 ccttttattt atcctttatt ctaattaaat atggaacata aacaatttaa aaaagaaata
     3601 gttgaaagaa catttttaat atatatcatt ttgttttcaa tccttttttt aattacaaat
     3661 cataaaattt tccctatttt gatacttatt tttgccttat caggtgctgt ttttcccaaa
     3721 gtgcctgtgt atctttataa atttggaaat tttattggtg aattatatac aaaacttatt
     3781 ctttcgataa tattttgttt tttaatctgt cctatttctt ttatcaaaaa attacaaaag
     3841 aagaaaattt tggaaaaaaa tagtttttgg gaagaaattg attttaaaat agaaaaagaa
     3901 aattttgaaa aaatgggata aaaatgaaac cgatatacat tttaggtata tctgcttttt
     3961 atcatgattc agcaagttgt ttacttgaag atgataaaat aatatctgct gttcaggaag
     4021 aaagatttac aagaactaaa cacgaccctt cttttccaat aaattcaatc aaattttgtc
     4081 ttgattatgc aaacttaaca cctgataaac ttgattatat tgtattctat gaaaagccat
     4141 ttataaaatt tgaaagatta cttgaaacac attttttaca tgcaccaaaa agtttaatac
     4201 aatttgtcca atcaatgcct ttatggttaa aagaaagaat cttaataaaa aatataatta
     4261 aaaaagaaat agaagaaaaa ttaaatgaaa aattatcacc gaatacaaaa attttattct
     4321 gccctcatca tctttctcat gcatcttctg cttttttccc atctcttttt gaaaaagcat
     4381 ccatattaac tattgatggt gttggagaat gggcaactgc cacacttgga tttgggaata
     4441 aaaataaaat aaatttcttc tatgagttaa aatttccgtc ttcagttgga cttctttatt
     4501 cttcttttac ttactatact ggatttaaag ttaacgatgg agaatataag ttaatgggtc
     4561 ttgcacctta tggaaatcct cattcagaaa aaacgaaaag atatataaag acaataaaag
     4621 aaaaattaat tgagataaaa gaagatggct ctataaaatt aaatatggat tattttgcat
     4681 ttaatacttc ttttaaaatg gttaatgaga aaaaatggat agaaactttt ggaattccac
     4741 cgagaaaacc agagagtaaa attttaactg aatatgcaga tatggcatat gccattcaga
     4801 atgttctgga agaaatttta ataaagatgg ctaaattttt gaaagaaatt acaaaagaaa
     4861 aatatttagt tattggtggt ggtgtgggac ttaattgtgt agcaaacagt aaacttctta
     4921 aagaaaaaat ttttgaagat atatgggttc aacctgctgc gactgatgca ggtggggcag
     4981 ttggtgctgc ttatgcagtt tatcatatgt atttagggaa agaaagaatt tgtaatggaa
     5041 aagataaaat ggaaggatgt tttttagggc ccgaatataa ttcttatgaa atagaaaaaa
     5101 ccttaaaaaa gtatgataat ttacaatttt ataaagaaga aaatcaggat aaattatgta
     5161 atcttattgc taaattgatt aacgaaaaga aaataattgg atggttttct ggaagaatgg
     5221 aatttggacc aagggctctt ggagcaagaa gtattcttgc tgatgcaaga gaccctgaaa
     5281 tgcaaaaaaa attaaatctt aaaataaaat ttagagaggg atttagacct tttgctcctt
     5341 ctgttttatg ggaagataca caagaatatt ttgaatggga taggccttct ccttatatgc
     5401 tttttacagc acctgttaaa gaaaatagaa gaaaaaacat accagaagat tattatgagt
     5461 gggatataaa agataaatta tattttatca ggtctgatgt tcctgcaata actcatattg
     5521 atttttcagc aagattacaa acagttcata aagaaacaaa ctataaattt tggaatctta
     5581 taaaaacttt taaagagctt actggctatt ctattataat aaatacttca ttcaatgtta
     5641 gaggtgaacc aattgtatgt acacctgaag atgctataaa atgtttttta aagacagaaa
     5701 tggattgttt agttatggaa gattttatta tttttaaaaa ggataagaag gagaaataat
     5761 gaaaaggaaa aaagaaatat caatttatca tataatgact gttttaataa ttcttacttt
     5821 cgttttttca ttttttatat tttatatact cacaaaagtt tgtataaatt tatctttaag
     5881 aattaccggt gcaagtataa tatgggaagg atcttctttt aaaataatac aaagaacgga
     5941 gagacattat agaaatttta tagaaaagat gattttttgt ttttcagata gatatacaca
     6001 attatttttg cctcaaataa gtaaattacc gagattaata aatcatccaa taattcaaaa
     6061 agaaatgcca catagtgaaa gagctttttt taaaaaacca aaaaatgaat acgtgagaca
     6121 tgcgacaatg gtttatactc catatgttca ttacgctcct gcacctggtt atatgggtgg
     6181 agtttttcat aataatcaac aattcagata tcctgagaat ttagacaaga aaaagataaa
     6241 tgaaataaga atttttataa caggtggttc tgctgcatgg ggttgtctca tgccagataa
     6301 taaatccaca atatcagctt ttttagaaga agaatttaaa aaatttccag ataaaaatta
     6361 tttctttagg gtaattaatg ctgcatgtgg aggatatcac actactgatg aaagaatttg
     6421 gatatttaat aggattacag aattcgaacc ggatgttata atttcatatt caggatataa
     6481 tgacatatat aatgtttaca gagttaaaca agatttattt tctgcgttac ataatgaagg
     6541 gtcttatttt tactgggctt atagagaata tgaatactac aacaataggg attttatatt
     6601 gaaaataaca cctgattttt ttcatttttt ttatactcca aacgattttc cacgaaaaac
     6661 cataaaaaat attcatataa tttcttctta tttaaattca attggagtta aatatatttt
     6721 tgtgcttcaa ccatttagaa gtgataatcc tgaagaatgt ttaattatgg cagagaaatt
     6781 agagaaagaa ttgaaacaag tatcaaaatt gtttaatttc aagtttatta gttacagaaa
     6841 ttttttttta aatcaagaag aatattttat agatttgtgt catttaggag acaaaggaaa
     6901 caaagttata gcttgtaatc tttatagaga actaatacct gttatttatg aaattataaa
     6961 aaataaaaaa ggagtctcta atgaaaaaaa acaaatttat tgaatatgag aaaaaggcaa
     7021 aagatttaag gaaattagtt cttgatatgt gtgttaaagc gggaactggt catgttactt
     7081 cttctttttc ctgtattgaa attcttgttg ctctttatta tggaggaatt ttaaaatata
     7141 aagtggataa tccaaaatgg gaaaaaaggg ataggtttat tttaagtaaa ggacaggcaa
     7201 gtcctatttt atatgtaatt ttagcagacc tgggtttttt tccgatttct gcctgtgata
     7261 aattttgttg tgcagatgga atgtttggag ttcatttaca gaatgatgta cctggtgtgg
     7321 aaataacaac tggctctctg ggacatggac ttggaattgg tgctggtatg gcttatattg
     7381 ctaaaaagga aaaaagggac tggtttgttt ttgtactact gggcgatgga gaattgtatg
     7441 aaggttctat atgggaatct gctctttttg tatctcatca taaattaaat aatcttgttg
     7501 ctattgttga cagaa
//
LOCUS       DAOSAT010000085         7444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_6407_length_7444_cov_4.456083, whole genome shotgun sequence.
ACCESSION   DAOSAT010000085 DAOSAT010000000
VERSION     DAOSAT010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6407_length_7444_cov_4.456083"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLF90_07420"
     CDS             complement(<1..287)
                     /locus_tag="PLF90_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57142.1"
                     /translation="MFWRGIPEEEKIKKVKNLGFPAFEFWGWKNKDIEKIKKAKEENG
                     LTLATFCFEPNGVLTTDEVKIEEIIKGAIETAKIAKILDCKKLILTTGNIV"
     gene            complement(327..1082)
                     /locus_tag="PLF90_07425"
     CDS             complement(327..1082)
                     /locus_tag="PLF90_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOK57143.1"
                     /translation="MNTHDFLELIKKDFGEMGPEEGLRFKYFDDEIKGIVVCWMVSKK
                     VLDFTVKNKFNLIVSHEDLYFPPEYAKGDGAGVISNYRKEIIEKNRINFVRLHYTVDK
                     NFIFDVFDKLSEGNLLINENFYRVYEFENKILKNLAKELKNKFKVKFLRITEPEKKVR
                     RAGCLVGGLGLSINSKFIDKILSYNVDTVIAGEVDEYTIRGLSDLKIGIIELGHEASE
                     TPGLIKFTEYLKKKLSELPVKYLKNTYPVKILK"
     gene            complement(1390..2172)
                     /locus_tag="PLF90_07430"
     CDS             complement(1390..2172)
                     /locus_tag="PLF90_07430"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HOK57144.1"
                     /translation="MEKRKRFLVDVGIEDLPYPIIVPSKLEKEGQHTVANISVKTRIM
                     QEFEPIWIDKFINILHKHRKKTKVIREIDEILKDLVEDLKTSSAEIMFLYPYFVEKTT
                     PVSKEKCLVKYTCSFAAKMSIAEENISNFFRIEIPVITTYPASSKDKPGGLFGQLSKV
                     ILTIQPAKDIYIEDIVELVEEHALVPIYSYHSEEDQTYLIKKIHSVEKTSVEMVDEIK
                     NELAKKKEIKWYSIYCYNYGMLHSYSTVITIEKSLWVPFSES"
     gene            complement(2176..3210)
                     /locus_tag="PLF90_07435"
     CDS             complement(2176..3210)
                     /locus_tag="PLF90_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57145.1"
                     /translation="MIVFFSNKFPEIEIIEFTKNHHRKRYAEKKDYLLKLYKKKNVEI
                     FIYNFEFTGDIIKEPFQKVNGNLLKKLSQIIPFAYEKISILYELTDLFYRNFPEKKHF
                     ILSDTGFFSTLPEYVSNYALPYEFVKKKIKRYGVSGLLHQWVYEKTNKTSNKKGNKVI
                     SVYLSDFPNICTIKNGKAIESSVGFTPVEGLPSIKTVGELDSQIIFFLYVCGFTMNEI
                     HEILTSKSGFSSILGKNCDIDDVVKNKKTRDILRYYILKHIGAYIGILNGIDRIIFSG
                     KKNNLILDFIKYCCEEMNFIEIKIKDKIRQGEDIFYLTDKESDVEVCFIKENRMEMIK
                     YYIEEIKKEE"
     gene            complement(3207..4421)
                     /locus_tag="PLF90_07440"
     CDS             complement(3207..4421)
                     /locus_tag="PLF90_07440"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOK57146.1"
                     /translation="MRLLVFNCGSSSLTFKILETDGKIYNVFLYGKAHRVGVKGIEES
                     FIEISYKDKCEKIVTSLRNHKESAEIVIDFLIKNSIKPDGIGHRFVHGGDYFKGNTLI
                     DEKNIDTLRKCLPLAPIHNSISLSVIEVCRRKYPDVREFVVFDNFFHKTIPEIHSRYL
                     LPKNLIEKFGFKKYGFHGLSYYYVTKKIYEILNLDIEKDELKVVACHLGTGGSSVCAI
                     KNGKSIDTSMGYSPNSGLMMSTRTGDIDPMLLFYIMYLYRINVEELIDLINKKSGILG
                     ISGFSSDLRDVINAILKEKKENAKLAFEMYTYRLKKYVSSYIFLLKGIDVLIFTDDIG
                     VKNPLVREKICENMDWAGIVIDKEKNKNINEKEINFIHKEESKVKIAVIPTDEEIVIA
                     WEGYKLIKEIIK"
     gene            complement(4473..7157)
                     /gene="ppdK"
                     /locus_tag="PLF90_07445"
     CDS             complement(4473..7157)
                     /gene="ppdK"
                     /locus_tag="PLF90_07445"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOK57147.1"
                     /translation="MAKTKKKKYIYFFGEGKAEGKGDMKDLLGGKGAGLAEMTNAGIP
                     VPPGFTITTEMCKYYYQHNEKLPEDFDEELKKYLKKLEKVTGKKFGDTKNPLLVSVRS
                     GAKFSMPGMMDTILNLGLNDEAVKGLAESTGNERFAYDAYRRFIQMFGNVVMGIDKNE
                     FEKILEEKKEEKGVHYDSELNAEDLKEIVEKYKKIYREKTGKDFPQDPMEQLKMAINA
                     VFKSWNNPRAITYRKLNKIPDDLGTAVNIVTMVFGNMGNDSGTGVGFTRNPSTGEKEF
                     YGEYLTNAQGEDVVAGIRTPKPISELEKEMPEVYKQLREITDRLEKHYRDVQDFEFTI
                     ERGKLYMLQTRTGKRTPLAAVKIAVDMVKEGLITKEEAIMRVEPSQIDQLLHPIIDPK
                     AKVEPVAKGLPASPGAASGKIVFTADKAEEIGKTEPVILVREETSPDDIHGMATAKGI
                     LTSRGGMTSHAAVVARGMGKPCVVGCGAIQIDENKGVMIISGKEVKEYDWITIDGGTG
                     NVMIGKVPTIEAGITGEFKTIMEWADEIRKLGIRANADIPRDAKKAREFGAEGIGLCR
                     TEHMFFAPERLPYVVEMIMAETEENRRKALDKLLPFQKEDFIGLFREMEGYPVIIRTL
                     DPPLHEFLPKREELMVEIALMKERKEDPEKIKEKEKILKRVEQFHEFNPMLGHRGCRL
                     GITYPEITEMQARAIMEAACELAKEGKTVIPEIMIPLVGNVEELKHQKEIVVRVAEEV
                     MKKYGVKIKYMVGTMIEVPRAALTADEIAKEADFFSFGTNDLTQMTLGFSRDDVGKFL
                     FYYLEKKILKDDPFMTLDQIGVGQLVKIGIEKGRKTNSKLEVGICGEHGGDPESVKFC
                     HRVGMNYVSCSPYRIPVAKLAAAQAVVEEKLKIEYTSK"
BASE COUNT         2210 a         1381 c          818 g         3035 t
ORIGIN      
        1 taaacaatat tacctgttgt cagtattaat tttttacaat caagtatttt agcaattttt
       61 gcagtttcaa ttgccccttt tataatttct tcaattttaa cttcatctgt agttaacact
      121 ccatttggtt caaaacaaaa agttgctaat gtcagaccgt tttcttcttt cgctttttta
      181 attttttcta tatctttatt tttccatccc cagaattcaa aagcaggaaa tccaagattt
      241 ttgacctttt ttattttttc ttcttcagga atacctctcc agaacatttc tatacataca
      301 gaaagttgaa ctatagtatc ctccttttat ttcaaaatct ttacaggata tgtatttttt
      361 aaatacttaa caggaagttc agacaatttt ttctttaaat attctgtaaa ttttattagt
      421 ccaggtgttt cacttgcttc atgaccaagt tctataattc caatttttaa atctgaaaga
      481 cctcttattg tatattcatc cacctctcct gcaataactg tatcaacatt ataagacaga
      541 attttatcaa taaattttga gtttatactt aatcctaaac ctccaacaag acaccctgct
      601 cttctgactt ttttttcagg ttctgttatt cttaaaaact taactttgaa tttatttttt
      661 aattcttttg caagattttt taatatttta ttttcaaatt cataaactct ataaaaattt
      721 tcatttatca aaagatttcc ttccgaaagt ttatcaaaaa catcaaaaat aaaattttta
      781 tccactgtgt aatgtaatct tacaaaattg attctgtttt tctcaataat ttcttttctg
      841 taattactta taactcctgc accatctcct tttgcatatt caggtggaaa gtataaatct
      901 tcatgtgaaa ctattagatt aaatttattc ttcactgtaa aatcaagtac tttttttgaa
      961 accatccagc atacaacaat tccttttatt tcatcatcaa aatatttaaa tcttaaacct
     1021 tcttccggtc ccatttctcc aaaatctttt tttataagtt ctaaaaaatc atgtgtattc
     1081 atttttaaac tcttgttcaa aataatataa aaaacttaat ttaattaatt gtgttcaatt
     1141 ttaccataat tttaaaaaat aaaaaaatga tttttaaatt ttttacaaaa ataagaataa
     1201 aaaattttcc tagatgatta aattgtctct tgattttttt attttattga aaaaacaact
     1261 caaataatct tcatcctatt tttaatgata tttataaatt ctacctttta aataataaat
     1321 tatgaagttt ataagttctc aaattatgaa tttattaagt tcttaagtta tgtaattatt
     1381 aagttattat tatgattctg aaaacgggac ccataaactc ttttctattg ttataacagt
     1441 gctgtaagaa tgaagcattc cgtagttata gcaatatata gagtaccatt ttatttcttt
     1501 tttctttgca agttcattct ttatttcatc taccatctct acacttgttt tttcaacaga
     1561 atgaattttt ttaataagat atgtttgatc ttcttctgag tgatatgaat aaattggaac
     1621 aagagcatgt tcttcaacta attcaacaat atcctctatg tatatatcct ttgcaggttg
     1681 tatcgttaaa ataactttac ttaactgtcc aaaaagtcca ccaggtttat cctttgaaga
     1741 agcaggatat gttgttatta cagggatttc aattctgaaa aaatttgaaa tgttttcttc
     1801 cgcaattgac attttagcag caaaagaaca ggtatattta accagacatt tttcttttga
     1861 aacaggggtt gttttttcca caaagtatgg atataaaaac attatttcag cacttgatgt
     1921 ttttaaatcc tctaccagat cttttagaat ctcatctatt tctcttataa cttttgtttt
     1981 ttttctgtgt ttgtgaagaa tatttataaa tttatcaatc caaatgggtt caaattcctg
     2041 cataatcctt gtttttactg aaatgttcgc cactgtgtgc tgtccctctt tttctaactt
     2101 ggaaggaact attattggat aaggtaaatc ttctattcca acatcaacaa gaaatctttt
     2161 tcttttttcc attttttatt cctccttttt tatttcttct atgtaatatt ttatcatttc
     2221 cattctgttt tcttttataa aacaaacttc cacatcagat tctttgtctg ttaaataaaa
     2281 aatgtcttct ccttgcctta ttttatcctt tatttttatc tctataaaat tcatttcttc
     2341 acaacagtat tttataaaat ctaaaattaa gttgttcttt ttccctgaaa atataattct
     2401 atcaatacca ttcaatattc caatatatgc ccctatatgt tttaaaatgt aatatcgcaa
     2461 tatatctctt gtttttttat tttttacaac atcatcaata tcacaatttt tgccaagaat
     2521 agaagaaaaa ccgctttttg aagttaaaat ttcatgaatc tcattcattg taaatccaca
     2581 tacataaagg aaaaatataa tctgagagtc cagttccccg actgttttaa tagagggtaa
     2641 tccttcaaca ggtgtaaatc caaccgaact ctctattgct ttgccatttt ttattgtaca
     2701 tatattggga aaatcactta aatatactga aattacttta tttccctttt tattgctagt
     2761 tttatttgtt ttctcgtaaa cccattgatg caaaagtcca gaaacaccat atctttttat
     2821 ttttttcttt acaaattcat aaggaagtgc ataatttgaa acatactccg gtaatgtgga
     2881 gaaaaaaccg gtatcagaaa gaataaaatg ttttttttct ggaaagtttc tataaaataa
     2941 atctgtcaat tcataaagaa ttgatatttt ttcataagca aaaggtataa tttgagaaag
     3001 tttttttaaa agatttccat ttactttttg aaaaggctcc ttaataatat cccccgtaaa
     3061 ttcaaagttg tagataaaaa tttcaacatt ctttttttta taaagtttta gcaaataatc
     3121 ttttttttct gcatatcttt ttctatgatg atttttggta aattcaatga tttcaatttc
     3181 tggaaattta ttactgaaaa acacaatcat ttaattatct cctttattaa tttataaccc
     3241 tcccatgcta ttacaatctc ttcatccgta ggaattactg caattttaac ttttgattct
     3301 tctttatgaa taaaatttat ctctttttca tttatatttt tatttttttc tttatcaatt
     3361 actattccag cccaatccat gttttcacat attttttccc ttacaagagg atttttaaca
     3421 cctatatcat ctgtaaaaat aagaacatct atacctttca ataaaaatat ataactgctt
     3481 acatattttt tcaatctgta tgtatacatc tcaaaagcaa gtttggcatt ttcttttttc
     3541 tcttttaaaa tggcatttat tacatccctc aaatcacttg aaaaaccaga tataccaaga
     3601 attccactct ttttattaat cagatctatg agttcctcta cgtttattct gtataagtac
     3661 ataatataga ataacaacat aggatcaata tctcctgttc gggtactcat catcaaaccg
     3721 gaatttggtg aataacccat ggaagtatca atggatttcc catttttaat tgcacagaca
     3781 cttgaacctc ctgttccaag atggcatgca acaactttta attcatcttt ttcaatatcc
     3841 aggtttagaa tttcatagat tttttttgtt acataataat aggataagcc atgaaatcca
     3901 tattttttga aaccaaattt ttctataaga ttttttggta atagatatct tgaatgtatt
     3961 tcgggtattg ttttgtgaaa aaaattatca aaaactacaa attcccgtac atcaggatat
     4021 ttccttctgc aaacttcaat gacactcaag gaaattgaat tatggatagg agccaaaggt
     4081 aaacattttc ttaaagtatc aatgtttttt tcatctatta aagtatttcc tttaaaataa
     4141 tcccctccat ggacaaatct atgccctatc ccatccggtt taattgaatt ttttattaaa
     4201 aaatctatta ctatttctgc tgattcttta tgatttctta aagaggttac aattttttcg
     4261 catttatcct tgtaagaaat ttcaataaaa gattcctcga ttcctttaac ccctactctg
     4321 tgtgctttac cataaaggaa aacattataa atttttccat cggtttcaag aattttaaaa
     4381 gttaaagatg aacttccaca attaaaaaca agtaatctca tcctttttat tttcttcttt
     4441 atctcacaga agatgtaatg ctttctattt atttacttgc ttgtatattc aatttttaat
     4501 ttttcttcaa ctactgcctg tgctgctgca agttttgcaa caggtattct ataaggagaa
     4561 caggatacat aattcatacc aaccctatga cagaatttta cactttcagg gtctcctcca
     4621 tgttctccgc atattccaac ttcaagttta gaatttgttt ttctcccctt ttctattccg
     4681 attttaacaa gttgtcctac acctatttga tcaagcgtca taaatgggtc atcttttaaa
     4741 attttctttt caagataata gaataaaaat tttccaacat catctctact gaatccaagg
     4801 gtcatctggg ttaaatcatt tgttccgaaa gaaaagaaat ctgcttcttt tgcaatttca
     4861 tcggcagtta aagcagcacg tgggacttct atcattgtac caaccatata ttttattttt
     4921 actccatatt ttttcattac ttcttctgca actctcacga ctatttcctt ttgatgctta
     4981 agttcttcta catttcctac aagtggtatc attatttcag gaattactgt ttttccttct
     5041 ttggcaagtt cacatgctgc ctccataatt gctcttgcct gcatttctgt gatttctgga
     5101 tatgttattc ctaatctaca acctctgtga ccaagcattg gattgaattc atgaaattgt
     5161 tcaactcttt taagtatttt ttctttctct tttatttttt cagggtcctc ttttctttct
     5221 ttcattaaag caatttctac cataagttct tctctttttg gtaaaaattc atgtaaaggt
     5281 gggtcaagag ttcttataat tacaggataa ccttccattt ctctaaataa cccaataaaa
     5341 tcttcttttt gaaatggtaa aagtttatca agtgcttttc ttctattttc ttctgtttct
     5401 gccataatca tttctacaac atagggtaat ctttcaggtg caaaaaacat atgttctgtt
     5461 ctgcaaaggc ctattccctc tgctccaaat tctctggctt tttttgcatc tcttggtata
     5521 tctgcatttg ctctaatacc taattttctt atttcatccg cccattccat tattgtttta
     5581 aattctccag ttatacctgc ttctatcgta ggaactttgc ctatcataac atttccagtt
     5641 cctccatcta tagtaatcca atcatattct ttaacttctt ttccgcttat aatcataact
     5701 cctttatttt catcaatttg tatagctcca catccaacta cacagggttt tcccattcct
     5761 cttgcaacta ctgctgcatg agaagtcatt cctcctctac ttgttaaaat tccttttgca
     5821 gttgccatac catgtatatc atcaggactt gtttcttctc ttacaagtat tactggttct
     5881 gtttttccaa tttcttctgc cttatctgct gtaaatacga tttttccact tgcagctcca
     5941 ggggatgcgg gaagtccttt tgctacaggt tccacctttg cttttgggtc aattatagga
     6001 tgaagtaatt ggtcaatttg agatggttct accctcatta ttgcctcttc ttttgttatc
     6061 aatccctctt ttaccatatc aaccgcaatt ttaaccgctg caaggggtgt tctttttcct
     6121 gttcttgtct gaagcatata taatttccct ctttcaatag taaactcaaa atcctggaca
     6181 tctctataat gtttttccaa tctatcagta atttctctaa gttgtttata tacttcaggc
     6241 atttcttttt caagttcact tattggtttt ggagttctta taccagcaac aacatcttct
     6301 ccctgcgcat ttgttaaata ttcaccgtaa aactcttttt ctcctgttga tggattcctt
     6361 gtaaatccca cacctgttcc tgaatcattg cccatatttc caaaaaccat agttactata
     6421 tttactgcgg ttccgaggtc gtccggaatt ttatttagtt ttctgtaagt tattgctctg
     6481 ggattattcc aagatttaaa aacagcattt atagccattt tcaattgttc catagggtcc
     6541 tgaggaaaat cttttcctgt tttttctcta taaatttttt tatatttttc aacaatttct
     6601 ttcaaatcct ctgcattaag ttccgaatca taatgtacac ctttttcctc ttttttttct
     6661 tcaagtattt tttcaaactc atttttatct atgcccataa caacatttcc aaacatctgg
     6721 ataaatcttc tgtaggcatc ataggcaaac ctttcgtttc ctgtactttc agcaagtcct
     6781 ttaactgctt catcattaag cccaagattt aaaattgtat ccatcatacc aggcattgaa
     6841 aatttagcac cggaccttac agaaaccaaa agcggatttt tagtatctcc aaatttttta
     6901 cctgtaacct tttcaagttt ttttaagtat tttttaagtt cttcatcaaa gtcctcagga
     6961 agtttttcat tgtgttgata gtaatatttg cacatttcag ttgtaatggt aaaaccagga
     7021 ggtacaggaa tccccgcgtt tgtcatttca gcaagtcctg caccctttcc tcccagtaaa
     7081 tccttcatat ctcctttacc ttctgctttt ccctctccaa aaaaataaat atattttttc
     7141 ttttttgttt ttgccatctt tttcctcctt ttaatttaaa ctatgattta atattgtcta
     7201 atttttgttt taaaagtgaa ctaaatattt tatcacaaaa atttagaaaa gtcaaaattt
     7261 atgtaaatat ttactaaacc ccatttactt ttttattttg ttatattttg cttttactgt
     7321 caactacact attttcttaa aggggtttaa acccctttaa aatccccaag aggtatcaac
     7381 tcccaaaatg gtggctcacc atctcgccac ccgtgctaat cggtgctcct tattcgtcgc
     7441 acca
//
LOCUS       DAOSAT010000086         7395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_6457_length_7395_cov_4.985422, whole genome shotgun sequence.
ACCESSION   DAOSAT010000086 DAOSAT010000000
VERSION     DAOSAT010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6457_length_7395_cov_4.985422"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /gene="rmuC"
                     /locus_tag="PLF90_07450"
     CDS             complement(<1..779)
                     /gene="rmuC"
                     /locus_tag="PLF90_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOK57148.1"
                     /translation="MLNLLLLLNLVLLIVIIFLIVTKKETDARDIILKQDEIKEKILT
                     ISKEFSENLLNTRHQLNQEITNILKNTEMLILDQVNKTISTLGTTDTNLQQRMENLRT
                     QLHQTLTDTVKNLTNSINSSTSQLNSNLDSKLEQLNKRLADTTGLITNLNLRVGELSK
                     SAEELRKVNEELKTALTSPKLGGDIGEQILENMLKNVLPQEIYKLQYLISPGNRVDAA
                     IFIENRFIPVDAKFPVISYKKMANSKDEEYEKNKQEFIKEV"
     gene            891..1778
                     /locus_tag="PLF90_07455"
     CDS             891..1778
                     /locus_tag="PLF90_07455"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK57149.1"
                     /translation="MRNNEIEWGRSLFTILISRGIKIKRVKVKRIIYFGFIFFICISF
                     ISVQSDNLVNECEMLEIAVKYEREGNLKKAIEIYQEIFDNTQKDIYKELSLENLGMIY
                     EKQKEYEKAIEIYKKCLDLVKVPFKICNTKIRIAYCYFYQKKYSDGFQILEEIIQEYP
                     STTFSADAQFAKASIYHIIIKDYEKAINEYEKMIKYYPDDWRVKNSLVLTKIASCYLE
                     NKEYDKSIEIYKKISSEYKNTPFEKYADLMIEFVNEYIKKGKEVPLEIIQKRMREVGL
                     GEGIVSEEVIDGKVIYKVK"
     gene            1834..3612
                     /locus_tag="PLF90_07460"
     CDS             1834..3612
                     /locus_tag="PLF90_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57150.1"
                     /translation="MCRNFFKIIFLILILEKIILFSDEIRNIDFIKESLKNQEKLSYP
                     SLKVKYSVSILIDGKESPHRKNCLYIRTPDYFFIEEQDLIKDEINFIRKLKHERSTKI
                     NHSYSITKTGYKAGRISQETESIFNTIKMETTNFPVLTEKNPFTPLYEALNEAIDTEE
                     KKIIDGYTCYLFKIIDRKSNICSNIWLGKDIEFCPVLIEVFRKNSILDHKIQFENYKE
                     IVKGFWFPMKVIVEIFPAETTPELENFYKSKGFSPDFVKKVLNEKSINIYNVIEISAG
                     KRISEEEIKLEFPPGTEVRDELTGRFFKITGLNSEEIIKLESIIKNYEYQSSLVKSIY
                     IKTKIYTQLNSFQENLTNTLENHTVVLARKGNLEYEEEISRFKPEYKINKIFTTKKLH
                     DKSTGIIKILGYSMNPENNEIISPPGLEGIITTDDNIVMKYKMFKTPDIGTKIIVFGL
                     PLLKRLKEDKVKLLPDTKKLDGYLCYIIEGDCSPPFEGKSYEIWIDPKIGFMPRLIKE
                     KISSKDYTIRKFLEYKEISKDIWFPMRVEEENVSEKEIKISPYTFISKIVRNLYVVDD
                     VKINTSGYSMINLFSILVKDSILVKK"
     gene            3759..3992
                     /locus_tag="PLF90_07465"
     CDS             3759..3992
                     /locus_tag="PLF90_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57151.1"
                     /translation="MYEREVKKFQKFGQDIWYPVEIERRFYDGKGQICQRDHKIIKKF
                     IPNSEVKDSLFTLKFPKGLLINDGIKGTGVFAK"
     gene            4018..6741
                     /locus_tag="PLF90_07470"
     CDS             4018..6741
                     /locus_tag="PLF90_07470"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOK57152.1"
                     /translation="MVRKLLRIVFLILILKGIILFSDDYEIKGRVVDRNGSGIKNALV
                     AYGPGVFWINYKSVRTDENGFFHLKNLPHEKSIVAVQCKGFSPKFFEITPGKEESIPF
                     LNVELEPGHSVSGYIIDENGKPVKGAQISTMALCRFISAGCEQIYRYLESSCITDEKG
                     YFKLEDLPSEGVFISVYAEGYAMIDKKFLGVDRDDYKIVLKVLGCIRGKIIDEKTKNP
                     VKKFKVKLNFPVLKKITDLTGGFSCDFIKGVNFESEDGTFEISNLTPDAVYKVIIESE
                     GFAPFYIEKVKAKRVQEKEYEIFELKPSFLRIEGYVYNSVKKTPVSDAEVIMIDTLGR
                     NIGFFSWINDYYDYEELGVVRKTRTDEKGKFTISDIRNTRGYFIIKHPEYGKAFYPEI
                     PITEKTKTIEFPIDPSGKIEGIVYDKNKSPISGVEIYLEQKIDGDSAFFGYVKTDENG
                     FYSFQNLSSGYYTIRVSSLNGVNYKEVYLNPGENKKVDFGLEKGAKIKGRVIHRGKPL
                     EEVEVSIIDIKKRTSIAKSTTDKNGEYEIIGLEKGEYKIEVMKGDWLDPNKLLIEKDI
                     KIKNEENEINFVFPESTIEGKVIDFKTKKPVSDIIISAYKKIRSYRLWGKNGFDTYEY
                     EWIWQPKQKAKTDKNGNFKLENVEDGMYVIVAGDINSKDRVASKLLRVKNPLSLKNII
                     LEINQPGKLKVELFDKETGKIIEKASVCLFTSENFYLHLGYPEVKYDDYSWFSMKNPY
                     SFKNGEIIFENLKPGIYYVFASAPGYVCSRKEKVVVKENGISEKNIKLGPGCMVEFIL
                     KEPEPPFNGILTIGLKLKTEDGRIPLIDYYGYRMSGIYEFFDEKDGKKVFVINTLKEG
                     RYTGEIEIYRENSLYVATYKFQKPLYRQKIEFSVEKGKKKVIEIDMREKRL"
     gene            6782..>7395
                     /locus_tag="PLF90_07475"
     CDS             6782..>7395
                     /locus_tag="PLF90_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57153.1"
                     /translation="MKIIKVKSGKKIHNWYPGKRECTSERILLNPYIGCEVGCFFCYA
                     LSYPGNFQKARKERVIFVYENFVENLKRQIEKLNIAFCGYLSPVSEPFQSVEKIYKLS
                     EKTIEFFVENNIPVEFITKSVIPESVVELIKQKKHSFGQISILTVDEDLRKKLMRRGA
                     ETELLFKNIEELAKNNIFCVCRTDPIIPFITDDREKLKILIKKAV"
BASE COUNT         2931 a          745 c         1276 g         2443 t
ORIGIN      
        1 ttaacctctt ttataaattc ttgcttattt ttttcatatt cttcatcttt tgaattagcc
       61 atttttttat aggatataac tggaaatttt gcatccactg gtataaatct gttttcaatg
      121 aaaatagcag catcaaccct atttccagga gaaattaaat actgaagttt atatatttct
      181 tgtggtagaa catttttcaa catattttca agtatctgtt cacctatatc tcctccaagt
      241 tttgggcttg taagggcagt tttaagttct tcatttactt ttcttaattc ttctgcagat
      301 ttgcttaatt caccaaccct taaatttaaa tttgttatta atcctgttgt atctgcaagt
      361 cgtttattta attgttcaag tttactatca agatttgaat taagttgaga agttgatgaa
      421 tttattgaat ttgtcaggtt tttcacagta tctgttaatg tttgatgtaa ttgtgttctt
      481 aaattttcca ttctctgctg taagtttgta tcagttgttc cgagtgttga gattgtttta
      541 tttacctggt caagaattaa catttcagta tttttaagaa tatttgttat ttcctgattt
      601 aactgatgtc ttgtatttaa aagattctca gaaaattcct ttgaaatggt tagtattttt
      661 tctttaattt catcctgctt taaaatgata tctctggcat ctgtttcttt ttttgtcaca
      721 ataagaaata taataactat cagaagaacc agattaagta gaagcaataa gttcagcata
      781 ttttaattat aactcaattt cattctaaaa tcaatgtatc aaggagacat cttttttcat
      841 taaagttatc tttgaagtga ataaaagtca ttcttgacga actttttaaa atgagaaata
      901 atgaaattga atggggaagg tctttattta caatattaat ttcaaggggt ataaaaataa
      961 aaagggttaa agtgaaaaga ataatatatt ttgggtttat tttttttatt tgtattagtt
     1021 ttatatctgt ccaatcagat aatttagtaa atgagtgtga gatgttagaa attgcagtga
     1081 aatatgagag agaggggaat ttaaaaaaag caattgaaat ttatcaggag atatttgata
     1141 atacacaaaa agacatttat aaagaactct cattagaaaa tctcggaatg atttatgaga
     1201 aacaaaaaga atatgaaaag gcaatagaaa tttataaaaa atgtcttgat ttagttaaag
     1261 taccatttaa aatatgtaat acaaaaatca gaattgctta ctgttatttt tatcaaaaaa
     1321 aatattctga tggatttcaa atcctggaag aaataataca ggaatatcca tcaaccacat
     1381 tttcagcaga tgcacaattt gcaaaagcat ctatttacca cattattata aaagattatg
     1441 aaaaagcgat aaatgaatac gaaaaaatga taaagtatta tccagatgat tggagagtta
     1501 aaaattcatt ggttctaaca aaaattgcaa gttgttattt agaaaataag gaatatgata
     1561 aatcaattga aatttataaa aagatatctt ctgaatataa aaatactccc tttgagaaat
     1621 atgcagattt gatgatagaa tttgtgaatg aatatattaa aaaaggaaaa gaagtccctc
     1681 ttgaaattat acagaagagg atgagagaag tgggattagg agaaggaata gtaagcgaag
     1741 aagttataga tggaaaagtg atttataagg taaaatgaga aggaggttat tttttattcc
     1801 aacaaagaca ataaggaaat aaaaaagggg aaaatgtgta gaaatttttt taaaattatt
     1861 tttctaatat taattttaga gaaaattatt ttgttttcag atgaaattcg gaatatagat
     1921 ttcataaaag agtctttgaa aaatcaagaa aaattaagtt atccttcatt aaaagtaaaa
     1981 tattctgtct caattttaat tgatggaaaa gaatctccac atagaaaaaa ttgtttatac
     2041 ataaggactc ctgattactt ttttattgaa gagcaagacc tgattaaaga tgaaattaat
     2101 tttattagaa aactgaaaca tgagaggagt acaaaaataa atcattctta ttcaataaca
     2161 aaaactggat ataaagcagg aaggatttct caggagacag aaagtatatt caacacgatt
     2221 aaaatggaaa ccaccaattt tccagtttta acagaaaaaa atccttttac tcctctttat
     2281 gaagcactga atgaggcaat agatacagaa gaaaaaaaga ttatagatgg ttatacttgt
     2341 tatttgttta aaataattga tagaaaaagc aacatttgtt caaatatatg gcttggaaaa
     2401 gatattgaat tctgtcctgt tttaattgaa gtttttagaa agaactctat tctggaccat
     2461 aaaatacagt ttgaaaatta caaagaaatt gtaaaaggtt tctggtttcc tatgaaagta
     2521 attgtagaaa tttttcctgc tgaaacaaca cctgaattag aaaattttta caaatctaaa
     2581 ggattttcac cagattttgt taaaaaagta cttaatgaaa aaagtattaa tatttataat
     2641 gttattgaaa tttctgcagg gaaacgaata tcagaagaag agataaaact ggaatttcct
     2701 cctggaacag aggtaagaga cgagttaaca ggaagatttt ttaaaattac tggtttaaac
     2761 tcagaagaaa ttataaagtt agaatctatt atcaaaaatt atgaatatca atcttctttg
     2821 gtaaaatcaa tatacataaa gacaaaaatt tatactcaac ttaactcttt tcaggaaaat
     2881 ttaactaata ctttagaaaa tcatacagtt gttttagcaa gaaaaggaaa tttagaatat
     2941 gaagaagaga taagcagatt taaaccagaa tataagataa ataaaatttt cacgacaaaa
     3001 aaacttcatg ataagagtac agggattata aaaattctgg gatattctat gaaccctgag
     3061 aataatgaaa taatttctcc tcctggatta gaaggtatta ttaccacaga tgacaacatt
     3121 gtaatgaaat ataaaatgtt taaaactcct gacataggta caaaaattat agtttttgga
     3181 ttaccattac taaaacgttt gaaagaagat aaagtaaaac ttttaccaga tactaaaaag
     3241 ttagatggat atttatgtta tataattgaa ggtgattgta gtcctccatt tgaagggaaa
     3301 agttatgaga tatggataga ccctaaaata ggatttatgc caagattaat taaagaaaaa
     3361 atttcatcaa aagattacac aattaggaaa tttttagaat ataaagaaat atcaaaagat
     3421 atttggtttc caatgagagt ggaagaagaa aacgtatcag agaaagagat taagatttct
     3481 ccttatactt ttatttccaa aattgttcgt aatttatatg tggtagatga tgtaaaaata
     3541 aatacttctg gttacagtat gataaactta ttttctatac tggttaaaga tagtatatta
     3601 gtaaaaaaat aataggagaa aaaattatag atggggatag gtgttatgaa atagaaattg
     3661 aaagagagtt tgaaatttat aaagtttatc tagatataga ttgtggatat ttaattgaaa
     3721 aagttgttgc tattgacaaa gaaaccaata atcaattaat gtatgaacgt gaggttaaaa
     3781 aatttcaaaa atttgggcaa gatatatggt atccagttga aattgaaaga aggttttatg
     3841 acgggaaagg acagatatgt cagagggacc acaaaattat taagaaattt ataccgaatt
     3901 cggaagtaaa agattcatta tttacactta agtttccaaa aggtctttta attaatgatg
     3961 gtatcaaagg aacaggagtt tttgctaaat agtgaaattt aaaataggag gagaaaaatg
     4021 gttagaaaat tgctcagaat tgtctttctg atattaattt taaaaggaat tatattgttt
     4081 tcagatgatt atgaaattaa aggaagggtt gtggacagga atggaagtgg tataaaaaat
     4141 gctttagttg cttatggtcc aggtgtattc tggattaatt ataaatctgt aaggacagat
     4201 gaaaatggct tttttcacct taaaaattta ccacatgaaa aaagtatagt tgctgttcaa
     4261 tgtaaaggtt tttccccaaa gttttttgaa attactcctg gaaaagaaga gagcattcct
     4321 tttttaaatg tagaacttga accaggacat tctgtctccg gttatataat tgatgagaat
     4381 ggtaaaccgg taaaaggcgc tcaaatttct acaatggctc tatgtaggtt tatttctgct
     4441 ggatgtgaac aaatttacag atatcttgaa tcatcctgta taacagatga aaaaggatat
     4501 tttaaactgg aagatttacc ttcagaaggg gtatttatct ctgtatatgc tgaaggatat
     4561 gcaatgattg ataagaaatt tcttggagtt gatagggatg attataaaat tgttttaaag
     4621 gtattaggat gtatcagggg taaaattatt gatgaaaaaa caaaaaatcc tgttaaaaaa
     4681 ttcaaagtaa aacttaattt tccagtttta aaaaaaataa ctgatttaac tggtggattc
     4741 agttgtgatt ttataaaagg tgtgaatttt gaaagtgaag atggaacttt tgaaatttca
     4801 aatcttacgc ctgatgctgt ttataaagta attattgaaa gtgaaggttt tgctccattt
     4861 tatattgaaa aagttaaagc aaaaagggtt caggaaaaag aatatgaaat atttgaatta
     4921 aagccatctt ttttaagaat agagggatat gtttacaatt cagtcaaaaa aactcctgtt
     4981 tcagatgctg aagtgattat gattgatact ctgggtagaa acattggctt tttttcctgg
     5041 ataaatgatt attatgatta tgaagaattg ggtgtggtaa gaaaaaccag aacagatgaa
     5101 aagggaaaat ttacaatttc agatattaga aatacaaggg gatattttat aatcaaacat
     5161 cctgaatatg gaaaagcatt ttatcctgaa attccaataa ctgaaaaaac aaaaactatt
     5221 gaatttccga ttgacccttc aggaaaaatt gaaggaatag tttatgataa aaataaaagt
     5281 cctatttcag gtgttgaaat ttatctggaa caaaaaatag atggagatag tgcttttttt
     5341 ggttatgtta aaactgatga aaatggtttt tattccttcc agaatttatc atcgggatat
     5401 tatacaataa gagtaagttc tttaaatgga gtgaattata aagaagttta tttaaatcca
     5461 ggagaaaata aaaaagttga ttttggtctt gaaaaaggag caaaaattaa aggaagggtt
     5521 attcatagag gaaaacctct tgaagaggtg gaggtaagta taattgatat taaaaagaga
     5581 acttctattg caaaatctac cacagataaa aacggagaat atgagataat tggacttgaa
     5641 aaaggagaat ataaaataga agtgatgaaa ggagattggt tagaccctaa taaactttta
     5701 attgaaaagg atataaaaat aaaaaatgaa gagaatgaga taaattttgt ttttcctgag
     5761 agtacaattg aagggaaggt tattgatttt aaaacaaaaa agcctgtttc agacataata
     5821 atcagtgcat ataaaaaaat aagaagttac aggttatggg gaaaaaatgg ttttgatact
     5881 tatgaatatg aatggatatg gcagccgaaa cagaaagcaa aaactgataa aaatgggaat
     5941 tttaaacttg aaaatgttga agatggaatg tatgttattg ttgcaggaga tattaattca
     6001 aaagatagag ttgcttcaaa actgttaaga gtaaaaaatc ctttatcatt gaaaaatata
     6061 attcttgaaa taaatcaacc aggtaaactt aaagttgaac tatttgataa agaaacaggt
     6121 aagataattg aaaaagcaag tgtgtgtctt tttacctctg aaaatttcta tcttcattta
     6181 ggttatccgg aagtaaaata cgatgattat tcctggtttt ctatgaaaaa tccttattca
     6241 tttaaaaatg gagagataat ttttgaaaat ttgaaacccg gaatatatta tgtatttgca
     6301 agtgcaccgg gatatgtctg ttcaagaaaa gaaaaagtag tggttaaaga aaatggaatt
     6361 tctgaaaaaa atataaaact tgggcctggt tgtatggttg agtttattct aaaagaacct
     6421 gaacctcctt ttaatggtat tttaactatt ggattgaaat tgaaaacaga ggatggaaga
     6481 ataccactta tagattatta tggatataga atgagtggaa tatatgaatt ttttgatgag
     6541 aaagacggaa aaaaagtttt tgttattaat acactgaaag aaggaagata tacaggagaa
     6601 attgaaattt acagagaaaa ttcactttat gtagcaactt acaaattcca aaaaccttta
     6661 tacagacaaa aaattgaatt cagcgttgaa aaagggaaga aaaaagtaat tgaaatagat
     6721 atgagggaaa aaaggttata ataaatatta taatctaact ttttatttta tcagtttacc
     6781 agtgaaaata ataaaagtaa aatcagggaa gaaaatacat aactggtatc ctggaaagag
     6841 ggaatgtaca tctgaaagaa tacttttaaa tccatatata ggttgtgaag ttggttgttt
     6901 tttctgttat gctttaagtt atccgggtaa ttttcaaaaa gcaaggaagg agagagtaat
     6961 ctttgtatat gagaattttg ttgagaattt aaaaagacag attgaaaaat tgaatattgc
     7021 tttctgtggt tatctttctc ctgtttcaga accatttcag tcagtagaaa aaatatacaa
     7081 attatcagaa aaaacaatag aattttttgt tgagaataat atacctgttg aattcattac
     7141 gaaatcagtt attccagaaa gtgtagttga attgataaaa caaaagaagc attcttttgg
     7201 tcagatttca attttaactg tagatgaaga tttaaggaag aaactgatga gaagaggagc
     7261 agagacagaa ttattattta aaaatattga ggaacttgca aaaaataata tattctgtgt
     7321 gtgtagaact gaccctatta ttccatttat taccgatgat agggaaaaac ttaaaatact
     7381 tattaaaaaa gcagt
//
LOCUS       DAOSAT010000087         7388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_6463_length_7388_cov_4.423292, whole genome shotgun sequence.
ACCESSION   DAOSAT010000087 DAOSAT010000000
VERSION     DAOSAT010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6463_length_7388_cov_4.423292"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1165
                     /gene="pnp"
                     /locus_tag="PLF90_07480"
     CDS             59..1165
                     /gene="pnp"
                     /locus_tag="PLF90_07480"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOK57154.1"
                     /translation="MTRGQTQCLASVTLGTSMDQQIVDGLYEETTKRFMVHYNFPPFS
                     VGEVSQLRAPSRREIGHGNLAERALLPMIPSEKVFPYTIRIVANILESNGSSSMATVC
                     AGSLSLMDAGVPIEKHVGGVSVGLIKNGDKYVILTDIQGEEDHYGDMDLKIAGTKDGI
                     TGFQMDVKITNLTFEILKEAVYQSKEARKEILDKMYSVISSPRTSLSPYAPKILTIKI
                     NPEKVGLVIGSGGKTIKKIIDETGVRIDILEEGEIRIYSNDMEACKKAASEINKIAEE
                     PEIGQIYEGVVTKIFPFGAVVKFLPSQEGLVHISELAPYRIKRVEDVVKIGSPIKVKV
                     IGIDEQGRVLLSRKQIEESQKKGDENEQGSKSKY"
     gene            1137..2852
                     /locus_tag="PLF90_07485"
     CDS             1137..2852
                     /locus_tag="PLF90_07485"
                     /inference="COORDINATES: protein motif:HMM:NF019496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57155.1"
                     /translation="MNKAVKANTNLSSYIKNVVIGVFAPCDPRIDEESRQRVKNIIEM
                     TANIIAQKVKLPDGSPCKVVYSDILIDGEKQADIVARQFENEKVNILVGVPDTWAFPQ
                     LTLISFLQQFPSDTPINLTCGNSGPKPGVVYTHACSGAISQYGKLIHIIVGNWPDTGL
                     NPVMSEETQKQLIDWCYAAITYTGLKGRRVVIFGHDSMGMETALAHIIPTRKIFGIEI
                     TRLDMKLLADMLNKEAYNKEELKELRNWLEKNSGGIEIRNEEDSKRLNQSLAMYLIVR
                     DIMKDLNAVGGGFMSQLEWGSDKRGIPLPVADIMESIFNSTFDHNGKKQPVPYATEAD
                     VQGLLTMLFFTYLSGGAPPLFMDFRKVWEGWEIKQLAEKIGIKIKGDEIWAVKGFVDG
                     DNSGSASFDWAGYPGEKPEEIMKRIKFPLADEFYFPGLGNSVTFVSPGGIKGIAGRLA
                     YSSLNNIFSMIWDEAETVELPEKLAEAVCKTSTYTWPHTFVVPKYASMIEYKQYAPAN
                     HFHMIWDLPVARLQYWMDLSNVLSVNPWKDRPEFIEGIDRPLPLLYLINGGETETKLK
                     IAQKF"
     gene            2873..4024
                     /locus_tag="PLF90_07490"
     CDS             2873..4024
                     /locus_tag="PLF90_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK57156.1"
                     /translation="MKKLRIGVIGVGGMGSAHCRSVKNLEETELTFVCDIDGNVAKSK
                     GEELGVKYFTDYKEAIKSGLCDGVIIATPHWFHPEIAIFAFENGLHVLSEKPIAVRVS
                     DADKMIKAAKKSGKVFTIMFQRRAEPLFKKAVEIIKSGALGEIQKSVCVDPWYRSQAY
                     YNSATWRATWIGEGGGVLINQAPHTIDLFTLLGGLPKKIYAKTRTKLHDIEVEDEVSA
                     LLEYKNGAWGYYYTSTCEPVGPLHMEFTGDKGKLVIRGSSLTFYSFEYPISESIVKVE
                     SMWGSLKYTEEKIEITSNIQTGHSVIIRNFARAVLYGEKLIVNGEEGLNSVEFINACI
                     LSGKKNKEVEIPVNRKEYDQLIEELKKSSKPKKVVKEERITDPSFAKKS"
     gene            4057..5655
                     /locus_tag="PLF90_07495"
     CDS             4057..5655
                     /locus_tag="PLF90_07495"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOK57157.1"
                     /translation="MEKKKLLIFIGEKPYHPSFELAKIIEKDLNEYEILIEKNKEIFS
                     KDEIFNYDGILIYADNTDLDEKEEEKLIQYIKNGGGLIGIHSTGAYFKKNKKFKEVLG
                     CEFISHSPVFPFELKSNNSHYINRRIPSTIIEDEFYFLNVKDDVEVLFNGYWQGKLLP
                     LGYIRNYGNGRIFYFACGHSLNVFENKDVIKAIKRGIFWSTRNEKQEREIKCGIIGYG
                     PAFGMGKYHADLINSTYGLKTVSFYDINKERVEQAKKDFPDSKTFTELDKFLNDKETE
                     LVVIITPHNTHRDLTIASLNAGKNVVVEKPMCIKLKEADEMIETAEKNNLMLSVFHNR
                     RWDGDFLKIEKIVKSGEIGKIFNIEIFMGGYGMPREWWRSEKEISGGIFYDWGAHFLY
                     WLLLLIPSKIKNVYGIIQKRKWFNFSNEDEVKSIITFENGEVADVQSSSIASIPKPRW
                     RILGEKGGIIQTDLIKVYRYENGMTEKIVQPLKSEIFRFYNSIADHLIFGEKAEIDVQ
                     IARNVIGIIDKTIESAEKGKIIEY"
     gene            complement(5652..6035)
                     /locus_tag="PLF90_07500"
     CDS             complement(5652..6035)
                     /locus_tag="PLF90_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57158.1"
                     /translation="MKKRGLGKDPLSWISSTIAEEREETIEEKKVESIEKKDEEKVEK
                     LPKFKTFEVRLTTLLREDQLEFLEKLIRDIHKNREPQYRKERITKNTLIRVFIDAFQN
                     VNFNIKNIPDEEELLKRVLEKILKD"
     gene            complement(6032..6799)
                     /locus_tag="PLF90_07505"
     CDS             complement(6032..6799)
                     /locus_tag="PLF90_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017531359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK57159.1"
                     /translation="MRRIAVINQKGGSGKTTTVVNTGYYLSELGKNVLLIDLDPQSHT
                     TIHLGFEPPQIGKSLYDLLVNQTPLSDVLKKTPYKNLDLIPSKIDLASAEIELVNEVG
                     REIILREILKKNKVKYDYIIMDCPPSLGLLTLNALTTAKEVFIPIQAEFFALEGLTKL
                     FQTIKIVKERLNPELEIKGIIITMYDKRKNICRDVERKVEEYFKDKVFKTRIRENVRL
                     AEAPSYGKPIGEFDSNSYGAEDYKKLVKEIIKQEKSL"
     gene            6949..7095
                     /locus_tag="PLF90_07510"
     CDS             6949..7095
                     /locus_tag="PLF90_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57160.1"
                     /translation="MKKNKYFELLVDSGTIYSVILKEELKKLIIKLTYFEEFVLANGE
                     TIEN"
     gene            7085..>7388
                     /locus_tag="PLF90_07515"
     CDS             7085..>7388
                     /locus_tag="PLF90_07515"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOK57161.1"
                     /translation="MRIKGTTKITGVFGYPISHSLSPIFQNTAFEYLGLDYVYIPLEV
                     KPKDLKKAVDSIRIFNWVGVNVTIPHKKEIVKYIDKLDESAEILGVVNTIHNINGKL"
BASE COUNT         2742 a          974 c         1327 g         2345 t
ORIGIN      
        1 aaataagacc gatagagtgt aaagttggac ttctaccgag aaatcatggg tctgctttat
       61 tacaagagga caaacacaat gccttgcttc tgttactctt ggtacgagta tggaccagca
      121 aatagttgat gggctttatg aagaaacaac aaaaagattt atggttcatt ataactttcc
      181 gccgttttct gttggtgaag tttcccagtt aagagctcct tccagaagag aaattggaca
      241 cggaaatctc gcagaaagag ctcttttacc aatgattcca tctgaaaaag tttttccgta
      301 tacaataaga atagttgcaa atatacttga atcaaatggt tcttcttcaa tggcaacagt
      361 ttgtgctggt agtttatccc ttatggatgc tggagttcct attgaaaaac atgtgggagg
      421 ggtatcagtt ggattgatta aaaatggaga taaatatgta atacttacag atattcaggg
      481 tgaagaggac cattacggag atatggattt aaaaatagct ggaacaaaag acgggataac
      541 aggattccag atggatgtta aaattaccaa tcttacgttt gaaattttaa aagaagcagt
      601 ttatcagtca aaagaagcaa gaaaagaaat ccttgataaa atgtacagtg ttatttcttc
      661 gccgagaact tctctttctc cctatgctcc aaaaatttta acaattaaaa ttaatcctga
      721 aaaagttggt cttgtaattg gttcaggagg aaaaacaata aaaaagataa tagacgaaac
      781 aggagttaga attgatatac ttgaagaagg tgaaataagg atatactcaa atgatatgga
      841 agcgtgtaaa aaagcagcaa gtgaaattaa taaaattgca gaagaacctg aaataggaca
      901 gatatatgaa ggtgtggtga caaaaatatt tccatttggt gcagttgtta aatttttacc
      961 ctctcaagaa ggacttgttc acatttctga acttgcacca tacaggatta aaagagttga
     1021 agatgtggtt aaaattggtt ctcctattaa agtcaaagta ataggcatag atgaacaggg
     1081 tagggttctc ttaagccgaa aacaaataga agaaagtcag aaaaaggggg atgaaaatga
     1141 acaaggcagt aaaagcaaat actaatcttt cttcatatat aaaaaatgtt gttattggtg
     1201 tttttgcccc atgtgatcca agaatagacg aagaatccag gcagagagta aaaaatataa
     1261 ttgaaatgac cgcaaatata attgctcaaa aagtaaaatt accagacggt tctccctgca
     1321 aagtggttta tagcgatatc ttaattgatg gagaaaaaca ggcagatata gttgcaagac
     1381 aatttgaaaa tgaaaaagta aatatacttg taggtgttcc cgatacatgg gcatttccgc
     1441 agttaactct tatttctttt ttgcaacaat ttccctcaga tacacctatt aacttaactt
     1501 gtggaaatag tggaccaaaa ccaggagtcg tttatactca cgcatgcagt ggagcaattt
     1561 ctcaatatgg taagttaatt catataattg ttggaaactg gcctgataca ggtttaaatc
     1621 ctgtaatgag tgaagaaaca caaaaacaac tcattgactg gtgttatgct gcaattacat
     1681 atacaggtct taaaggtaga agagttgtta tttttggcca tgattctatg ggaatggaaa
     1741 ctgctcttgc ccatatcatt ccaacaagaa aaatttttgg aattgaaata actcgtcttg
     1801 atatgaaact tcttgctgat atgttgaata aagaagcata taataaagaa gaacttaaag
     1861 aattaagaaa ctggcttgaa aaaaattcag gtggaattga aattagaaat gaagaggata
     1921 gtaaaagatt aaatcaatca cttgcaatgt atcttatagt cagagatata atgaaggatt
     1981 taaatgctgt tggtggtggt tttatgagtc aacttgaatg gggttcagat aaaagaggga
     2041 ttcctttacc cgttgcagat attatggagt caatttttaa ctccacattt gaccataatg
     2101 gtaaaaaaca accagttcca tatgcaacag aagcagatgt tcagggactt ttaacaatgc
     2161 ttttctttac atatctttca ggaggagcac ctccattatt tatggatttc cgaaaggtct
     2221 gggaaggatg ggaaattaaa caacttgcag agaaaattgg tataaaaata aaaggtgatg
     2281 aaatatgggc agttaaagga tttgtagatg gagataattc tggaagtgct tcttttgact
     2341 gggcaggtta tccgggagaa aaacctgaag aaataatgaa aagaattaag tttcctctcg
     2401 ctgatgaatt ttactttcca ggccttggaa attctgttac atttgtttca cctggtggaa
     2461 ttaaaggtat tgcaggtcgt cttgcttaca gttcattaaa taatatattc tcaatgatat
     2521 gggatgaggc cgaaacggtt gaacttcctg aaaaacttgc agaagcagtt tgtaaaactt
     2581 caacatatac atggcctcat acatttgtag tccctaaata tgcaagtatg attgagtata
     2641 aacagtatgc tccagcaaat catttccata tgatatggga tttacctgtt gcacgacttc
     2701 aatactggat ggatttatca aatgtattat ctgtaaatcc atggaaagat agaccagaat
     2761 tcattgaagg tattgacaga cctttaccac tactctatct tataaatggt ggtgaaacag
     2821 aaacgaaatt aaaaatagca cagaaatttt aaagatcaaa aggaggatga aaatgaaaaa
     2881 attaagaata ggagttatag gagttggtgg aatgggttct gctcattgta gaagtgttaa
     2941 aaatcttgaa gaaactgaac taacatttgt ttgtgatata gacggaaatg ttgcaaaaag
     3001 taaaggagaa gaattaggag ttaaatattt tactgattat aaagaagcaa taaaaagtgg
     3061 attatgtgac ggtgtaataa ttgcaactcc ccactggttt catcctgaaa tagcaatatt
     3121 tgcttttgaa aatggacttc atgttttaag cgaaaaacca attgccgtaa gagtttcaga
     3181 tgcagacaaa atgataaaag cagcaaaaaa atcaggtaaa gttttcacaa ttatgtttca
     3241 aagaagagca gaaccactat ttaaaaaagc agtggaaatt attaaaagcg gggcacttgg
     3301 agaaattcag aaaagtgtat gtgtagaccc atggtataga agtcaggcat attataatag
     3361 tgcaacatgg agagcaacat ggattggaga aggaggaggt gttttaataa accaggcgcc
     3421 tcatacaatt gatttgttta ctcttcttgg aggcttacct aaaaaaattt atgcaaaaac
     3481 aagaacaaaa ctccatgata tagaagtaga agatgaagta agtgcgcttc ttgaatataa
     3541 gaatggtgca tggggatatt attatacatc tacctgtgag cctgttggac cgttacatat
     3601 ggaattcaca ggagataaag gaaaacttgt aatcagagga agttctctta ctttttactc
     3661 ttttgaatat ccaataagtg aatcaattgt taaagtagaa agtatgtggg gctctttaaa
     3721 atatactgaa gaaaagattg aaattacatc aaatattcaa acaggtcatt ctgtaataat
     3781 aagaaatttt gccagagcgg ttctttatgg agaaaaactg attgtaaatg gagaagaagg
     3841 attaaacagt gtagaattta taaatgcttg tatactttca ggaaaaaaga ataaagaggt
     3901 tgaaatacct gttaatagaa aagaatatga ccaattaata gaagagttaa agaaatcatc
     3961 aaaaccaaaa aaagtcgtta aagaagaaag aattacagac ccttcatttg caaaaaaatc
     4021 ttaaatttat tttattttga ataaaaaagg agaaaaatgg aaaaaaagaa attattaatt
     4081 tttatcggcg aaaaacctta tcatccttct tttgaacttg caaagataat agaaaaagac
     4141 ctaaatgaat atgaaatttt aattgaaaaa aacaaagaaa tcttttcaaa agatgaaata
     4201 tttaattacg atggaatttt aatttatgca gacaatacag atttagatga aaaagaagaa
     4261 gaaaaactga ttcagtatat caaaaatgga ggagggctta taggaattca ttcaacaggt
     4321 gcttatttca aaaaaaataa aaagtttaaa gaagttcttg gatgtgaatt tatatctcat
     4381 agtcctgtct ttccatttga acttaaatca aataattctc attatataaa tagaagaata
     4441 ccatcaacaa taattgaaga cgagttttac tttttaaatg taaaagacga tgtagaagtt
     4501 ttatttaatg gttactggca gggaaaatta ttacctcttg gctacataag aaattatgga
     4561 aacggaagaa ttttttattt tgcctgtggt catagtttaa atgtttttga aaataaggat
     4621 gttataaagg caattaaaag gggaattttc tggtcaacaa gaaatgaaaa acaagaaaga
     4681 gagataaagt gtggaattat tggatatggg cctgcttttg gtatgggtaa atatcatgcg
     4741 gatttaataa attctacata tggattaaaa acagtttctt tttatgatat taataaagaa
     4801 agagttgaac aagcaaaaaa ggactttcca gatagtaaaa cttttacaga gttagataaa
     4861 tttctgaatg ataaagaaac agaacttgtt gtgattataa ctccacacaa tacacataga
     4921 gacctcacta ttgcttctct aaacgcagga aaaaatgttg tagtggaaaa accaatgtgt
     4981 ataaaattaa aagaagcgga tgaaatgata gaaactgcag aaaaaaataa cttaatgctt
     5041 tctgtctttc ataacaggag atgggatggt gattttctta aaatagaaaa aattgttaaa
     5101 tcaggtgaaa tagggaaaat ttttaatatt gaaattttta tgggtggata tggaatgcca
     5161 agagaatggt ggagaagtga aaaagaaatt tcaggtggaa ttttttatga ctggggagca
     5221 cattttcttt actggttatt acttcttatc ccttcaaaaa taaaaaatgt ttatggaatt
     5281 attcaaaaaa gaaaatggtt taacttttca aatgaagatg aagttaaaag tataataact
     5341 tttgaaaatg gagaagtagc agatgttcaa tcatcttcta ttgcatcaat cccaaaaccg
     5401 agatggagaa tacttggaga aaaaggagga attattcaga cagatttaat aaaggtttac
     5461 agatatgaaa atggaatgac tgaaaaaata gttcaaccct taaaaagtga aatattcaga
     5521 ttttataaca gtattgcaga ccatttaatt tttggagaaa aagccgaaat agatgttcag
     5581 atagcgagaa atgttatagg aataattgat aaaaccattg aatcagcaga aaaagggaaa
     5641 attatagaat attaatcctt taaaattttt tcaaggaccc tttttaaaag ttcttcttcg
     5701 tcaggaatat ttttgatatt gaaatttaca ttttgaaaag catctataaa aacccttata
     5761 agtgtatttt tagtaattct ttcttttctg tactgtggtt ctctgttttt gtgaatatct
     5821 ctgataagtt tttcaagaaa ttcaagttgg tcttctctta ataatgtagt aagccgcact
     5881 tcaaatgtct taaatttagg caatttttca actttttctt catctttttt ttcaatactt
     5941 tcaacttttt tttcttcaat agtttcttct ctttcttctg ctattgttga acttatccaa
     6001 cttaaagggt cctttcctaa acctcttttt ttcataaact tttctcctgt tttattattt
     6061 ctttaacaag ttttttataa tcttctgctc cataagaatt actatcaaat tcaccaattg
     6121 gttttccgta acttggtgct tctgcaagac gaacattttc tcttattctt gttttaaaaa
     6181 ccttatcttt aaaatattct tcaactttcc tttcaacatc cctgcatata ttttttcttt
     6241 tatcatacat tgtaataata attcctttaa tttcaagttc aggattcaat ctttctttca
     6301 ctatttttat tgtctgaaaa agttttgtta atccttcaag tgcaaaaaac tctgcctgta
     6361 ttggtataaa aacttccttt gcagtggtta aagcatttaa agttaaaagt ccaagagaag
     6421 ggggacaatc cataatgatg taatcatatt tcaccttgtt tttcttaagt atctctctta
     6481 aaattatttc ccttcccact tcattaacaa gttcaatttc tgcacttgca aggtcaattt
     6541 ttgatggtat aaggtcaaga tttttgtaag gagttttctt taaaacatct gaaagaggtg
     6601 tttgatttac aagtaaatca tagagtgatt tacctatttg gggtggttca aaaccaagat
     6661 gaattgtagt atgtgattga gggtcaaggt ctataagtaa tacatttttc ccaagttctg
     6721 aaagataata tccagtattt actacagtcg ttgttttccc tgaaccacct ttttgattta
     6781 taaccgctat tcttctcata tccctccttt cataagttca taacttctta atttatttta
     6841 aacaaattta aagtaatgtc aaataaaatt tatttttata gcaaaaagag gtaaaataaa
     6901 aaacaaaagg agtcaaaaca ggaataattt ttctaaaaat aaaggtttat gaaaaaaaat
     6961 aaatattttg aattgcttgt tgattcaggc acaatatatt cggttattct gaaagaagaa
     7021 ttgaaaaaac ttataattaa actaacttat tttgaggaat ttgtccttgc aaatggagaa
     7081 accattgaga attaaaggaa ctacaaaaat aacaggtgtt tttggatacc ctattagtca
     7141 ttctttatca cctatttttc agaatactgc ttttgaatat cttggacttg attatgtata
     7201 tattcctctt gaagtaaaac caaaagacct aaaaaaagca gtagattcaa taagaatttt
     7261 taattgggta ggagtaaatg tgacaattcc tcataaaaaa gaaattgtaa aatatattga
     7321 taaacttgat gagtcagcag aaattcttgg agttgtaaat accattcata acattaatgg
     7381 aaaattaa
//
LOCUS       DAOSAT010000088         7275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_6572_length_7275_cov_5.464958, whole genome shotgun sequence.
ACCESSION   DAOSAT010000088 DAOSAT010000000
VERSION     DAOSAT010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6572_length_7275_cov_5.464958"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3029)
                     /gene="rpoB"
                     /locus_tag="PLF90_07520"
     CDS             complement(<1..3029)
                     /gene="rpoB"
                     /locus_tag="PLF90_07520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOK57162.1"
                     /translation="MELVEIPDLLSIQKKSYEEFLQKDVPPEKRKIKGLEEVFRKVFP
                     VESEDGLLKLEYISYSIEDPSNTIEECIEEKLTYEGKLKVKFRLLINRKTEGGSFHNV
                     GIKEQVVYVGGIPLMTESGSFVINGIERVIVNQLLRCPGVYFKEEEVILQKTLYSAKI
                     YPSRGLWIEIHVDQHDTLWVLIGKKKVLATTFLKAMGLTEEEIIKEICGEQYRSIEEL
                     PEKSVLKITFLKDNTKDKEDALKNIYTELRPGYPPIIKEAEKFFNSLFFTNETYDLSE
                     AGRSQINKKLGINLKERHLTGLDILKTIKYLLNLYEKNEGDTDDIDHLGNKRVRASAE
                     IIKEHVYEGLVRLARYCKEKMALVDKSKIEDISPQELINHRFFVNVVNDFFARNQLSQ
                     FLDKTNPLAEITHKRRITAVGEGGVKERKRAGFEVRDVHYTHFGKICPIETPEGANIG
                     LINSLTVYAKIDELGFLRSPYFKVKNGYVKRDKVEYLSADEEEKYNIAPSDIPYDEKT
                     GLISPETVIVRGKEGNFIEVKREEIDYIGVSPVQIVGVSASLIPFLEHDDSNRALMGS
                     NMQRQSVPPIKPEPPIVKTGMEKYVVRDCGAVIKAKNPGIVEYVDAEKIIVKRDNPGL
                     FPWEEKDVYYLKKFKKTNQDTCFHQRPVVNVGDVVKENDILTDGFAISKTGELSLGKN
                     VLVAFMPWHGYNYEDAILLSERLVKDDVFTTIHIKEFEVEARDTELGPEEITSDLPNV
                     PEEQLVNLDEEGIVKIGTEVEPDDILVGKVTPRGESELTPEEKLLRVIFGEKSKNVLN
                     TSERVPPGVKGVVINVRKYKRREDVSEEEIEKEVKQMLKSFKEKEKIVKDIITEEIKK
                     FLSSKNLKIKVTSGVSGWEKLINKVPDNIREDLIKIIEIGKSEIGKIEIQAREEELKI
                     RKGYELDPDVRMKVVVEIATKKKIAAGDKMSGRHGNKGVIAKILPEEDMPFLEDGTPV
                     DIVLNPLGVPSRMNVGQILETHLGWALKTLGYSMEVPV"
     gene            complement(3105..3503)
                     /gene="rplL"
                     /locus_tag="PLF90_07525"
     CDS             complement(3105..3503)
                     /gene="rplL"
                     /locus_tag="PLF90_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOK57163.1"
                     /translation="MENVEKNVIDEIVEKIEKLSVLEVAELAKKLEEKFGVSGMVAPV
                     GVISASAQPAATSAPAEEKTTFDVILKNAGSNKIQTIKEVRAITNLGLKEAKDLVESL
                     PKPIKEKVSKEEAEEIKKKFDAIGAEVEIK"
     gene            complement(3505..3693)
                     /locus_tag="PLF90_07530"
     CDS             complement(3505..3693)
                     /locus_tag="PLF90_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57164.1"
                     /translation="MNGEVIEKDKIDQIGKLPGKKELQGMVITMIKMPTVRLVNSIKS
                     PMTKLINVLNQIKSKKES"
     gene            complement(3750..4016)
                     /gene="rplJ"
                     /locus_tag="PLF90_07535"
     CDS             complement(3750..4016)
                     /gene="rplJ"
                     /locus_tag="PLF90_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOK57165.1"
                     /translation="MKKWTKKRKEEVVKKLTEEFKNSKINILGSFSNLTVSEMQKIRD
                     SIKEFGGNVMVVKNNLIDIVFQNLSKDEVCKFISGPSFVIWSSI"
     gene            complement(4018..4719)
                     /gene="rplA"
                     /locus_tag="PLF90_07540"
     CDS             complement(4018..4719)
                     /gene="rplA"
                     /locus_tag="PLF90_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOK57166.1"
                     /translation="MSKRYSALLEKVDRTKLYDIDEAIEVLKGLHSAEFDESVDLAIK
                     LGVDPKKLQQPVRGSVILPNGTGKEIKVLVFASGENLKKALDSGADYAGGSEIAEKIK
                     NGWLDFDYVIATPDMMKVVAPLGKILGPRGLMPNPKTGTVTDNIENAVKEAKKGKVDF
                     KMDKNGNMHFSVGKISFPSDKLKENIISAILAVLSVKPQGVKGSYIKSIHLSLTMSPS
                     VKLNANKIIQELKGV"
     gene            complement(4731..5162)
                     /gene="rplK"
                     /locus_tag="PLF90_07545"
     CDS             complement(4731..5162)
                     /gene="rplK"
                     /locus_tag="PLF90_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOK57167.1"
                     /translation="MPAEKKVKAIVKLQIPAGQATPAPPVGPALGQHGVNIMEFCKQF
                     NNVTKGKEGTTIPVVLTVYEDRSFTFIVKTPPTSELIKKAIKIVKGSSIPNREKVGRI
                     TRKQIEEIAKIKLPDLNTKDLQKAVKIIEGSCKSMGVEIVD"
     gene            complement(5183..5716)
                     /gene="nusG"
                     /locus_tag="PLF90_07550"
     CDS             complement(5183..5716)
                     /gene="nusG"
                     /locus_tag="PLF90_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOK57168.1"
                     /translation="MSKWYILHVRTGQEKRIKKLLENRVNDDKKRINQIVIPEEEVLE
                     VEKGEKKAKSRRFWQGYMLIELDDDSNNEIVWHKIKTTPGVFGFLGAGETPVPLKEEE
                     VEEIQKEIDERKAKPLPKVEFKVKDRIEIIDGPFVGYTGIVEEVYPERERLKVSVSIF
                     GRATSLELNFWQVEKIT"
     gene            complement(5721..5906)
                     /gene="secE"
                     /locus_tag="PLF90_07555"
     CDS             complement(5721..5906)
                     /gene="secE"
                     /locus_tag="PLF90_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOK57169.1"
                     /translation="MKDKIKNYLFEIRSELKKVTWPEKKILRLSTVIIVFLMVIMALY
                     FGIVDLIFSKVVSLIIR"
     gene            complement(5923..5996)
                     /locus_tag="PLF90_07560"
     tRNA            complement(5923..5996)
                     /locus_tag="PLF90_07560"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5960..5962),aa:Trp,seq:cca)
     gene            complement(6095..6244)
                     /gene="rpmG"
                     /locus_tag="PLF90_07565"
     CDS             complement(6095..6244)
                     /gene="rpmG"
                     /locus_tag="PLF90_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOK57170.1"
                     /translation="MREIVSLVCSECKNRNYYTTKNKKVKKDKLQLSKYCPNCKKHTL
                     HTEGK"
     gene            complement(6254..>7275)
                     /gene="tuf"
                     /locus_tag="PLF90_07570"
     CDS             complement(6254..>7275)
                     /gene="tuf"
                     /locus_tag="PLF90_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOK57171.1"
                     /translation="TINIVHIEYETDKRHYAHIDCPGHADYIKNMITGAAQMDGAILV
                     VSAPDGPMPQTREHILLARQVDVPAMVVFMNKMDMMEDKELVELVELEVRELLSKYGF
                     PGEEIPIIKGSALKVLECGCSKRECQWCGKIWELMDAIDNYIPEPVRVIDKPFLLAVE
                     DVFSITGRGTVATGRIERGKVKVGEEVEIIGLSHDIKRSVVTGLEMFRKELDEGVAGD
                     NVGVLLRGIEKDEVERGMVVAAPGSITPHTRFKAQVYVLKKEEGGRHTPFFTGYRPQF
                     YMRTTDVTGEIKLPEGVEMVMPGDNIEMEIKLIYPVALEKGQRFAIREGGKTVGAGAV
                     TEIIE"
BASE COUNT         2025 a         1594 c          798 g         2858 t
ORIGIN      
        1 acaggaactt ccatgctata accaagagtt ttcaaagccc aaccaagatg tgtttcaaga
       61 atctgtccaa cattcattct tgaaggaact ccaagtggat ttaaaacaat atcaacagga
      121 gttccgtctt caagaaaagg catatcctct tcaggtaaaa tttttgcaat aactccttta
      181 tttccgtgtc ttccactcat tttatctcca gctgcaattt tctttttagt tgcaatttca
      241 accacaactt tcattctaac atcagggtca agttcatatc cctttcttat ttttaactct
      301 tcttctcttg cctgtatttc aatctttcct atttcactct tccctatttc tattattttt
      361 atcaaatctt ctcttatatt gtctggaact ttattaatta acttttccca tccagatact
      421 cctgatgtca ctttaatttt taaattttta ctgctgagga atttttttat ctcttctgtt
      481 ataatatcct taacaatttt ttctttttcc ttaaaagatt tcaacatctg ttttacttct
      541 ttctctattt cttcttccga aacatcttct ctccttttat attttctgac atttataaca
      601 actcctttta ctccgggtgg aactctttca gaagtattta aaacattttt tgacttttct
      661 ccaaaaatta ctcttaaaag tttttcttca ggagttaatt ctgactctcc tcttggcgtg
      721 actttaccaa caagtatatc atcaggttca acttcagttc ctattttaac tatcccttct
      781 tcatcaagat ttacaagttg ctcttctgga acattcggta ggtctgatgt aatttcttca
      841 ggaccgagtt ctgtatctct tgcttcaact tcaaattctt taatgtgtat tgtggtaaaa
      901 acatcatctt taaccaatct ttcacttaat aatatagcat cctcataatt atagccatgc
      961 cagggcataa aagcaacaag aacatttttc ccaagagaaa gttctccagt ttttgaaatt
     1021 gcaaaaccat ctgtaagaat atcattttct ttaactacat ctccaacatt aactacaggt
     1081 ctctgatgaa aacaagtatc ctgatttgtt ttcttaaact ttttcagata ataaacatct
     1141 ttttcttccc aggggaataa cccgggattg tctcttttca caataatttt ctctgcatca
     1201 acatattcaa caattccagg gttttttgcc ttaataactg caccacaatc tcttacaaca
     1261 tacttttcca ttcctgtttt tacaattggt ggttcaggtt taatgggcgg aactgactgt
     1321 ctctgcatat ttgaacccat caatgctctg tttgaatcgt catgctcaag gaaaggaatt
     1381 aaagaagcac tcacaccaac tatctgaaca ggcgaaacac ctatataatc aatttcctct
     1441 cttttaactt ctataaaatt accttcttta ccgcgaacaa taacagtttc aggagaaata
     1501 agtcctgttt tttcatcata aggtatatca gaaggagcaa tattatactt ttcttcttca
     1561 tctgcactta aatattcaac tttatctctt ttaacatatc cattcttaac tttaaaataa
     1621 ggggacctta aaaatccgag ttcatctatt tttgcataaa cagttaaaga atttatcaag
     1681 ccaatattag ctccttcagg tgtttctatg ggacatattt tcccaaaatg agtataatgg
     1741 acatctctta cttcaaatcc tgctcttttt ctttccttaa ctccaccttc tccaactgct
     1801 gtaattcttc ttttatgagt tatttctgca agtggatttg ttttatcaag aaactgagat
     1861 aattgatttc ttgcaaagaa atcatttaca acatttacga aaaatctatg atttatcaat
     1921 tcctgagggg aaatatcttc aattttactt ttatcaacaa gtgccatttt ctccttacag
     1981 taccttgcaa gacgaacaag tccttcataa acatgttcct taataatttc tgcggatgct
     2041 cttactcttt tatttcccag atgatctata tcgtctgtat ctccttcgtt tttttcataa
     2101 agatttaaaa ggtattttat tgtttttaaa atatcaagcc ctgttaaatg tctttctttc
     2161 agattaatcc caagtttttt atttatctga cttctacctg cttctgaaag gtcatatgtc
     2221 tcatttgtaa aaaataatga attaaagaat ttttctgctt cttttattat tggtgggtat
     2281 cccggtctta attcagtata tatatttttt aaagcatcct ctttatcctt tgtgttatcc
     2341 ttcaaaaaag ttatttttaa tacggacttt tcaggtaatt cttcaatact tctatattgt
     2401 tctccgcata tctcttttat aatttcttct tctgtcagcc ccattgcttt caaaaaagtt
     2461 gttgcaagga cctttttttt acctatcaaa acccaaagag tgtcatgctg gtctacatgg
     2521 atttcaatcc ataaacctct tgaaggataa atttttgctg aatataatgt tttctggaga
     2581 attacttcct cttctttaaa ataaactcca ggacatctta aaagttgatt aacaataact
     2641 ctttcaattc catttattac aaaacttcca ctttctgtca ttaaaggtat gccaccaaca
     2701 taaacaacct gttcttttat acctacatta tggaaactgc ctccttctgt ttttctattt
     2761 attaaaagac gaaatttaac ttttaattta ccttcatatg ttaatttttc ttctatgcat
     2821 tcttcaatag tatttgaagg gtcttcaatt gaataggaaa tatattcaag ttttaataat
     2881 ccatcttcac tttcaacagg aaaaactttt ctgaaaactt cctccaaccc ctttattttt
     2941 cttttttcag gaggaacatc cttctgcaaa aattcttcat aagatttttt ctggatactc
     3001 aacaaatctg gaatttcaac taattccatt tttactcctt tttaaaatta aaaatcaaaa
     3061 ataaaaatat caaatgtttt tttccccttt ttcaaaaaat aagtttattt tatttcaact
     3121 tctgctccaa tagcatcaaa ctttttcttt atttcttccg cttcttcctt actaactttc
     3181 tcttttattg gttttggcaa tgattctact aaatcctttg cttctttcaa accaagattt
     3241 gtaattgctc ttacttcttt tattgtctga attttgtttg aaccagcatt tttaagtatt
     3301 acatcaaatg ttgtcttttc ctctgcagga gcagaagttg cagcaggttg tgcagaagca
     3361 ctgataacac caacaggagc aaccatccca cttacaccaa acttttcttc aagttttttt
     3421 gcaagttcag caacttccag aactgaaagt ttttcaattt tttctactat ttcatcaata
     3481 acattttttt ctacattttc catttcaact ctccttttta cttttaattt gatttaaaac
     3541 atttattaac tttgtcattg gacttttaat tgaatttacc aatcttactg ttggcatttt
     3601 tatcatagta attaccatac cctgtaattc ttttttacct ggcagttttc caatttggtc
     3661 aattttatct ttttctatta cttcgccatt caataaacca ccttttattt ctattttccc
     3721 tgtgtctttt tgaaaattta aaactgattt tatatactgc tccagataac aaaacttgga
     3781 ccactgataa atttgcaaac ttcatctttg cttaagttct gaaaaactat atcaatcaaa
     3841 ttgtttttta caaccataac attaccacca aattccttaa tagaatccct tatcttttgc
     3901 atctctgaaa ccgttaagtt tgaaaagctg cctaaaatat taattttact atttttaaat
     3961 tcttctgtca actttttcac aacctcctct tttctctttt ttgtccattt tttcatttta
     4021 aactcccttt aattcctgta ttattttatt agcattaagt tttacagaag gggacattgt
     4081 aagagaaagg tgtattgatt taatataact tccttttaca ccctgtggtt ttacactcaa
     4141 tacagcaaga attgcagaaa ttatgttttc tttaagttta tcggatggaa aagaaatttt
     4201 acctacagaa aaatgcatat ttccattttt atccatttta aaatcaactt tacctttttt
     4261 tgcttcttta actgcatttt caatattatc tgtaactgtg cctgttttag gatttggcat
     4321 taaacctctt ggaccaagaa ttttaccaag aggtgcaaca accttcatca tatcaggcgt
     4381 agcaataaca taatcaaaat caagccatcc attttttatc ttttcagcaa tttcactacc
     4441 acctgcataa tcagcaccgg aatcaagtgc ttttttgaga ttttctccac ttgcaaatac
     4501 gagaactttt atttcttttc ctgttccatt tggtaatatt actgaacctc taactggctg
     4561 ctgaagtttc ttagggtcaa ctccaagttt aattgcaagg tcaacgcttt catcaaattc
     4621 ggctgaatga agacctttta aaacttctat tgcctcatca atatcgtata attttgttct
     4681 gtcaaccttt tcaagtaaag cagagtatct ttttgacata gtttctcctt ttaatcaaca
     4741 atttctactc ccatactttt acaggaacct tctattattt ttactgcctt ctgtaaatcc
     4801 tttgtgttta aatctggtaa tttaattttt gctatttctt caatctgttt tcttgttatc
     4861 ctgccaactt tttccctgtt gggaatagaa gaacctttga ctatcttaat tgcctttttt
     4921 attaattctg aagttggagg tgttttaact ataaatgtaa aacttctatc ttcataaaca
     4981 gtcaaaacaa cagggattgt tgttccttct tttcccttcg taacattatt aaattgttta
     5041 cagaactcca taatatttac gccatgctga ccaagtgcag gtccaacagg aggagcagga
     5101 gttgcctgtc ctgctggtat ctgcaattta actattgcct ttactttttt ttctgctggc
     5161 attttattac tcctttatag atttatgtaa ttttttcaac ctgccagaaa tttagttcaa
     5221 gagatgttgc tcttccaaaa attgatacac ttacttttaa acgttctctc tcaggataaa
     5281 cctcttcaac aattcctgta taccctacaa aagggccatc tataatctca attctatctt
     5341 ttactttaaa ttcaaccttc ggtaatggtt ttgcctttct ctcatctatt tccttttgta
     5401 tttcttctac ctcttcttct tttaaaggaa caggggtctc tcctgctcca agaaatccaa
     5461 aaactcctgg tgttgttttt attttatgcc agacaatttc attatttgaa tcatcatcaa
     5521 gttcaataag catatatcct tgccagaacc ttctactttt tgcttttttc tctccctttt
     5581 caacttcaag gacttcttct tctggaataa caatctgatt tattcttttt ttgtcgtcat
     5641 taaccctatt ctcaagaagt ttctttattc tcttttcctg ccctgttcta acatgtaaaa
     5701 tataccattt actcattttt tcaccttatt attaaactga caacttttga aaaaattaag
     5761 tccactattc caaaataaag cgccataata accataagaa aaactattat cactgttgat
     5821 aaccttaaaa tttttttttc aggccaggta acttttttca attctgacct aatttcaaat
     5881 aaataatttt ttattttatc tttcataaaa attcctgtca agcaggcctg ggaggactcg
     5941 aacctccaac ctacggattt ggagtccgcc gctctcccaa ttagagctac aggcctattt
     6001 tcccaatcac tttattgttt aaatttttaa aaataaattt tgcaaaaaaa tattatactt
     6061 ctaattttta tttctgtcaa gttcacaaaa ttttttactt accttctgta tgtaaagtat
     6121 gttttttaca gttgggacaa tacttactca actgtaattt atccttctta acttttttat
     6181 ttttagttgt ataatagttt ctatttttac attccgaaca aaccaaagaa actatttctc
     6241 tcattttact cctttattca attatttcag taacagcacc agcacctact gtcttccctc
     6301 cttctcttat tgcaaacctc tgtccttttt caagtgctac cggatatatc aattttatct
     6361 ccatctctat attatcccca ggcataacca tctctacccc ttcaggcaac tttatctccc
     6421 ctgtaacatc tgtcgtcctc atgtaaaact gtggcctata tcctgtgaaa aatggtgtat
     6481 gccttccacc ttcttctttc ttcaatacat atacctgcgc cttaaatctt gtatgtggtg
     6541 ttatactacc aggtgctgct actaccatac ccctttctac ttcatccttc tctatacccc
     6601 ttaataatac acctacattg tcccctgcta ctccttcatc caattccttt ctaaacatct
     6661 caagacctgt aactacactc ctctttatat catgacttaa tcctattatc tctacttcct
     6721 cccctacttt taccttaccc ctctctatcc tccctgtcgc tactgtaccc ctacctgtta
     6781 tgctaaatac atcctctacc gctaacaaaa atggcttgtc tattaccctt acaggctccg
     6841 gtatataatt gtctatcgca tccattaact cccatatctt cccacaccac tgacattccc
     6901 tctttgaaca tccacactct aataccttta atgcactccc ttttattatc ggtatctctt
     6961 cccccggaaa tccatacttg ctcaataact cccttacctc taattccact aactccacta
     7021 actccttatc ctccatcata tccatcttgt tcataaatac taccatcgcc ggtacatcta
     7081 cctgcctggc taataatatg tgctcccttg tctgcggcat cggaccatct ggcgcactca
     7141 ctaccaatat cgctccatcc atctgcgctg cacctgttat catgttcttt atataatccg
     7201 catgaccagg acaatctata tgcgcataat gcctcttgtc tgtctcatac tctatatgca
     7261 ctatgtttat agtca
//
LOCUS       DAOSAT010000089         7273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_6574_length_7273_cov_5.711832, whole genome shotgun sequence.
ACCESSION   DAOSAT010000089 DAOSAT010000000
VERSION     DAOSAT010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6574_length_7273_cov_5.711832"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..909)
                     /locus_tag="PLF90_07575"
     CDS             complement(157..909)
                     /locus_tag="PLF90_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOK57172.1"
                     /translation="MKFEIFGGNLQFVKVYISQGEEIYAEAGKMMFKSSNVNMETRMT
                     SQSTKDLLKDVLTRTITGESIFMTYFTCYDKEGYVAFAGDFPGKIQAIELKPGISFIA
                     QRDAFICAESRVKFGITFQKKVGPAFFGGEGFILEKFTGPGTVFIHAGGDFYEIDLKE
                     NEKIHIDTGCIVGFEETVDYDVEFVKDIKTAIFGGEGLFLATLTGPGKIILQSMTLAR
                     LRRELGGFGLKKGGGEKFGLGGLLGGILGSED"
     gene            complement(906..2822)
                     /locus_tag="PLF90_07580"
     CDS             complement(906..2822)
                     /locus_tag="PLF90_07580"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOK57173.1"
                     /translation="MRFQKDTLSNEDLKKLEDLRKNCIRDILTMTTIANSGHPGGAIS
                     SLDIYLIVFSYANISPLKIDDPERDKIVVSHGHTSAGVYSVLGRLGFFDIDMAISTFR
                     KTGSIFEGHIVRKVPGVEWGTGNLGQGLSAGCGFVLADKLNNRNSYTFVLMSDGEQTK
                     GQVGEARRFAIKYGLNKLKVIIDYNKIQISGKTDKVMPCRIKENYLADGWEVIECDGH
                     DFTQLYNCIKKSLSIDKPTCILAHTVMGKGISFMENEYKYHGKPLSEEEYLKAMEELK
                     LEPVLEKYKKMREKDWKFPERKFYFSPSINTGKPIVYGKEEKTDNRTAYGKALLDIAK
                     INKENKEYPVIVFDCDLASSVKTDFFEKEFPERFFQVGVQEHNAATISGAISTDKFIT
                     FFSDFGVFGIDETFNQQRLNDQNFTNLKLVCTHLGLDVGEDGKTHQCIDYIGVLRNLY
                     GFKIIIPADPNQTDRVIRYIADKPGNWFVGMGRSRIPVILKENGEVFFDENYKFEYGR
                     ADIIREGKDGYILTYGCMVYRAIKVHEKLKENDVDIGIMNFSCPTVIDEEKIKIAINT
                     GLIITYEDHHIDTGIGSTVALYLAEKSIKVNFLRFGIKNYGASGNPDELFKKEGISPE
                     DIVEKIIKFKKEVL"
     gene            complement(2835..3881)
                     /locus_tag="PLF90_07585"
     CDS             complement(2835..3881)
                     /locus_tag="PLF90_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOK57174.1"
                     /translation="MDRRICAYYNYKGDVFVEEEPIPELKEGEILVKVEASLISPGTE
                     IGGIRNLRISPAPTKFEKRRFGYANSGVIVEIGKKCKGFKIGQRVACMGSGYALHTNY
                     ACIPQNLCFPLPDELSFEEGTFCHLSATALWAVKRGKLEIGENVVVAGLGIIGQIVSQ
                     LSKLSGCHVIGWDKYDLRMEKAVQNGIDRVINIEKEDLVRQTIEFSRNYGIDTGFICF
                     GGEATETIKLLVETMKTAPDTHKMGKIVIVGLVSVNLNFPVYLGNIDILSSSRTGPGY
                     HDEKWEYGEDYPPVFVQWSTKRNVEEILIFIKEKRLNVKSLITDIFPLTEIASACEKL
                     INHPEKTLGVILKP"
     gene            complement(3893..4942)
                     /locus_tag="PLF90_07590"
     CDS             complement(3893..4942)
                     /locus_tag="PLF90_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57175.1"
                     /translation="MNREKVKNGILKGSPIKRHIILLILTYIAVINLTVLFYLITPKY
                     YRATSTILQPPESEVSLSSSQTAKFSKEPLSLIRTNTELFFSILNSRRMKDDIIEKFN
                     LKKHYGVSNDDEAIEILNERTNIFLTKDKVIQIDVIDRDPKIAAGIANFYTENLDYLI
                     KELTITTAKQNRIFIEKRLKETTELIEELEKRLTTIQNTNKLVIDQDLTQISQTGGKL
                     MEQLINKQLELEKERQILDDKDPKIIMLKNEIKEIQSSLAKLLGSQDELTRILRELKV
                     QESLYNFLTSKMEEAKIEETRDTPVVQILDKAKVPYKVYKPDIKFILLLVSSIIGGIG
                     FLIIFFDFLRFLGTI"
     gene            complement(4939..6828)
                     /locus_tag="PLF90_07595"
     CDS             complement(4939..6828)
                     /locus_tag="PLF90_07595"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOK57176.1"
                     /translation="MKIEKFYRIIKIDWSHFLKKLVFTFIICFFSFKLNCEVFSGYPE
                     LKIFGEDFFENYTPLLKTHQLPLSDDYILGPGDTLIINVWGFFEQEYQKEVETDGSIF
                     ISGIGKIYLAGKPLKEVKKIIEDKFYKKYKNIQVSVSGGKIRTINIYILGEVKKPGVY
                     EIFPFLNIIDVIAIAGGPNKNGSLRRIEIIKNDGGKELIDIYPILLKGEKPKVFQFQS
                     GDTVFVHQSENLVGITGGVRRPAIYELKNMELKELIDLSGGFLPVADISHIQIERIDK
                     EKGRVLIDIKEKDILNLSLKNFDVVKVPLLSSQSFYQVSITGAVKTQRVYGWKEGIKI
                     SNILKKDDLLPFAETEKAEIIRIENGYRKIISFSPEKLFSGDTNNDLNLLPQDKIVIY
                     SKERPEKKVVITGEVKYPGEYVIESGEKISNIIKRAGNFTSSAYPKGIVFLRESVKKQ
                     KEEEIEKLLKEKRETLNSALKTAQNTEEKQVIEKTLIAVEKLAEVKPQGRIIIKMDDF
                     EKFENSVYDISLENGDIIYIPKKPVYVSVVGEVNNPANVLYEPDLTLNDYIQKTGGFT
                     KDADRKSIFIVRVDGSSDRNLEKISEGDTIIIPFEPRGEKLRFIKDIMQIFYQIAVGV
                     GVLIK"
     gene            6972..>7273
                     /locus_tag="PLF90_07600"
     CDS             6972..>7273
                     /locus_tag="PLF90_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57177.1"
                     /translation="MEEIKDIKLETEADLDLFGNPAKIRIVATSDKLYIIPEKGFQEK
                     ISFDLKRIESFNIRQTVGSAFFQIKIDGYFIDIFRFTNSNRYKFSRFITQVQNLKSG"
BASE COUNT         2283 a         1317 c          842 g         2831 t
ORIGIN      
        1 aagttccttt tcaaatttaa ttttttcttc ttctttcagt gaaattcttg ctaacttact
       61 caaatattca acatcttctt tttttattac ctttttctcc ataaaaaaat tataaaatta
      121 aacttaattt aagtcaaatt ttaaacttta gaaaaattaa tcctcactgc ctaaaatacc
      181 accaagtaaa cctccgagtc caaatttttc tcctccgcct tttttcaatc cgaaacctcc
      241 aagttctctt cttaatcttg caagggtcat actctgtaag attatctttc caggaccggt
      301 taatgttgca agaaataatc cctctccgcc aaaaattgct gtttttatat cctttacaaa
      361 ctcaacgtca taatcaactg tttcttcaaa tccaactata caacctgtat caatatgaat
      421 tttttcattt tcttttaaat caatttcata aaaatctccg cctgcatgaa taaaaactgt
      481 tccaggacct gtgaattttt caagtataaa accttctcct ccaaaaaagg caggacctac
      541 ttttttctga aaagtaattc caaattttac tctgctttca gcacatataa aagcatctct
      601 ctgggcaata aaagaaattc ctggttttaa ctcaatcgcc tgaattttac cggggaaatc
      661 tcccgcaaaa gcaacatagc cctctttatc ataacaggta aaataagtca taaaaattga
      721 ttcaccagta attgtcctgg ttaaaacatc ttttaaaagg tcttttgttg attgagatgt
      781 cattctggtt tccatgttta cattactact tttaaacatc atttttcctg cttcagcata
      841 aatttcttct ccctgagaaa tatacacttt tacaaattga agattaccac caaaaatttc
      901 aaatttcata atacctcctt tttaaacttt ataatttttt caacaatatc ttcaggagat
      961 ataccttctt ttttaaataa ttcatcagga ttcccagaag caccataatt tttaatccca
     1021 aatctcaaaa aattaacctt tatacttttt tcagcaagat atagagcaac tgttgaacca
     1081 attcctgtat ctatatgatg gtcttcatag gttattataa gtcctgtatt tatggcaatt
     1141 tttatctttt cttcatcaat cactgtcgga caactgaaat tcataattcc tatatctaca
     1201 tcattttctt ttaatttctc atgaacttta atagctctgt aaaccataca tccataagtt
     1261 agaatatatc catctttacc ttctcttatt atgtctgccc tgccatattc aaatttataa
     1321 ttctcatcaa aaaatacttc accattttct ttcaatatca caggaattct acttcttccc
     1381 attccaacaa accagtttcc cggtttgtcc gcaatatacc ttataaccct gtctgtttga
     1441 tttgggtctg ctggaattat aattttaaat ccatataagt ttcttaaaac tccgatataa
     1501 tcaatgcact gatgtgtttt cccatcttct ccaacatcaa gtccaaggtg cgtgcataca
     1561 agttttaaat tagtaaaatt ctggtcatta agtcgttgtt gattgaatgt ttcatcaata
     1621 ccaaatacac caaaatcaga aaaaaatgtt atgaatttat ccgttgagat tgctccactt
     1681 attgttgctg cattatgttc ctgaacacct acctggaaaa atctttctgg gaattccttt
     1741 tcaaaaaaat ctgtttttac acttgaagca aggtcacaat caaaaacaat tacgggatac
     1801 tctttatttt ccttattaat ctttgctata tcaagtagtg cttttccata tgctgttctg
     1861 ttatctgttt tttcttcttt accatagaca atcggctttc cagtgttaat tgaaggagaa
     1921 aaataaaatt ttctttcggg gaatttccaa tctttttctc tcatcttttt gtatttttcc
     1981 aatacaggtt caagtttaag ttcctccatc gcttttaaat attcttcttc tgataatggt
     2041 tttccatgat atttatattc attttccata aaagaaattc ctttacccat tactgtatga
     2101 gcaagtatac aggttggttt atcaattgat aaagattttt ttatacaatt atatagttgg
     2161 gtaaaatcat gaccatcaca ttcaataact tcccacccat ctgcaagata attttctttg
     2221 attctgcaag gcataacttt atcagttttt ccacttatct gaattttgtt gtagtcaata
     2281 attactttta gtttattcaa tccatattta atagcaaatc ttctcgcttc accaacttga
     2341 ccttttgttt gttcaccatc actcatcaaa acaaaagtat atgaatttct attgttgagt
     2401 ttatctgcaa ggacaaaacc acatcctgca gaaagtccct gtccaagatt tcctgttccc
     2461 cattcaactc caggcacttt tcttacaata tgtccttcaa aaatacttcc tgtttttctg
     2521 aatgtactga ttgccatatc aatgtcaaaa aaaccgagac gtccaagaac agaataaaca
     2581 cctgcagatg tatgtccatg acttacaact attttatctc tttcagggtc atctattttt
     2641 aaaggtgaaa tatttgcata agaaaaaaca ataagataaa tatcaagaga agaaattgct
     2701 cctccgggat ggcctgaatt tgcaattgtt gtcattgtta gaatatctct tatgcaattt
     2761 tttcttaaat cttcaagttt tttcaagtcc tcatttgaga gagtatcttt ctgaaatctc
     2821 atattccctc ctttttatgg ttttaatatc acacctaagg ttttttctgg atgatttatt
     2881 aatttttcac atgctgatgc tatttctgtt aatgggaaaa tatctgttat aagtgattta
     2941 acatttaatc ttttttcttt tatgaaaatt aaaatttctt caacattcct ttttgtactc
     3001 cattgaacaa aaactggtgg ataatcttct ccgtattccc atttttcatc atggtaacca
     3061 ggtccagtcc ttgaagatga aagtatatca atatttccta aataaactgg aaaatttaaa
     3121 tttacagaaa ccaatccaac aatgactatc tttcccattt tatgtgtatc cggtgctgtc
     3181 ttcatagttt caacaagtaa ttttatagtt tcagttgctt ctccaccaaa acagataaat
     3241 cctgtatcta ttccgtaatt tcttgaaaat tcaattgttt gtctgactaa atcttctttt
     3301 tcaatattaa tcactctatc tataccattc tggactgctt tttccattct taaatcatat
     3361 ttatcccacc caattacatg acatcctgaa agtttagata actgactgac aatctgacct
     3421 ataattccca aacctgccac tacaacattt tcccctattt caagttttcc tcttttgaca
     3481 gcccagagtg cagttgcaga taaatggcag aaagttcctt cttcaaaact taattcatca
     3541 ggtaatggaa aacataaatt ctgaggaata caggcataat ttgtgtgtaa agcatatcca
     3601 gaacccatac aggcaactct ctgacctatt ttaaatcctt tacatttttt acctatttca
     3661 actattactc ctgaattagc atatccaaat cttcttttct caaattttgt tggagcaggg
     3721 gagattcgta agtttctaat tccacctatt tctgttccgg gacttattaa acttgcctca
     3781 actttaacaa gaatttcccc ttctttcaat tcaggaatag gttcttcttc aacaaaaaca
     3841 tctcctttgt aattataata agcacatatt cttctatcca taaaaacctt tcctatatag
     3901 ttccaagaaa tcttaagaag tcaaaaaata taattagaaa tccaattccg cctataatac
     3961 tgcttacaag aagtaatata aattttatat caggtttgta aaccttataa ggaacttttg
     4021 ctttatctag aatctgaaca acaggagtat ctcttgtttc ttctattttt gcttcttcca
     4081 tttttgaagt gagaaaatta tatagagatt cctgaacttt taattctctt aaaattcttg
     4141 ttaattcatc ctgagagcca agtaattttg caagagaaga ttgtatttct tttatttcat
     4201 ttttaagcat tataatttta gggtctttat catctaaaat ctgtctttct ttttcaagtt
     4261 caagttgttt atttataagt tgttccataa gtttaccgcc tgtttgtgat atttgagtta
     4321 aatcctggtc aataacaagt ttattggtat tctgaattgt agttagtcgt ttttccaatt
     4381 cctcaataag ttcagttgtt tctttcaatc ttttttcaat gaatatcctg ttttgttttg
     4441 ctgtggtaat tgttaattct tttattaaat aatcaaggtt ttctgtataa aaatttgcta
     4501 ttcctgcggc gattttcggg tctctatcaa taacatctat ttgaattact ttatcttttg
     4561 ttaagaaaat gttagttctt tcattaagta tttctattgc ctcatcatca tttgatacac
     4621 cgtaatgttt tttgagatta aatttctcaa ttatatcgtc tttcatcctt ctactgttaa
     4681 ggatggagaa aaaaagttct gtatttgttc ttataagaga aaggggttct tttgaaaatt
     4741 ttgctgtttg tgaggaagat aaactgactt ctgattcagg aggttgcaaa atagtagatg
     4801 ttgctctgta atatttaggt gtaattaaat aaaagagcac tgtaaggttt attaccgcga
     4861 tatatgttag aataagtaaa attatatgtc tttttatagg acttcccttt aaaattccat
     4921 tttttacttt ttctctattc attttattaa cactcctact ccaaccgcta tttgatagaa
     4981 aatttgcatt atatctttaa tgaatcttaa tttctctcct cttggttcaa aaggaataat
     5041 aatggtgtct ccttcactga ttttttctaa atttctatca cttgaaccgt caactctaac
     5101 aataaaaata ctttttctat cagcatcttt tgtaaagcca cctgtctttt gaatataatc
     5161 attcagagtt aaatcaggtt catacaatac atttgctgga ttattgactt ctccaactac
     5221 tgaaacatat acaggttttt tgggtatata aataatatct ccgttttcaa gagaaatatc
     5281 ataaacacta ttttcaaatt tttcaaaatc atccattttt atgattattc ttccctgtgg
     5341 tttaacttct gcaagttttt caaccgctat taaagttttt tcaattacct gtttttcttc
     5401 tgtgttttga gcagttttta atgcagaatt caatgtttct cttttttctt ttaatagttt
     5461 ttctatttct tcttcttttt gttttttcac cgattccctt aaaaatacaa ttccttttgg
     5521 ataagcagaa gaagtaaaat tacctgctct ttttataatg ttgcttattt tttctccact
     5581 ttcaattaca tattctccag ggtactttac ttctccggta attacaactt ttttttcagg
     5641 tctttctttt gaataaatta caattttatc ctgaggtaaa agatttaaat cattatttgt
     5701 atctcctgaa aaaagttttt caggagaaaa agaaataatt tttctatatc cattttcaat
     5761 tcttataatt tctgcttttt ctgtttcagc aaatggtaat aaatcatctt tttttaaaat
     5821 attacttatt ttaatccctt ctttccatcc ataaactctc tgtgttttta cagcacctgt
     5881 tattgaaacc tgataaaaag attgagaact taataatgga acttttacta catcaaaatt
     5941 ttttaaagag agatttaaga tatctttctc ctttatatca attaacactc tccctttttc
     6001 tttatcaatt ctttcaattt gaatatgaga aatatctgca accggtaaaa aaccacctga
     6061 taaatcaatc aattctttta attccatatt tttcaattca tatattgcag gtcttcgtac
     6121 tccacctgta attccgacaa gattttctga ttgatgaaca aaaacagtgt ctccgctctg
     6181 gaattgaaaa acttttggtt tttcaccttt aagaagaatt ggatagatgt caataagttc
     6241 ttttcctcca tcatttttaa ttatttcaat tcttcttaaa ctaccatttt tatttggacc
     6301 acctgctatg gcaattacat ctattatgtt taaaaaagga aaaatttcat aaactcctgg
     6361 ttttttaact tctccaagaa tataaatatt aattgttcta attttccctc ccgaaacaga
     6421 gacctgaata ttcttatatt ttttatagaa tttatcctca attatttttt tgacttcctt
     6481 cagtggtttt cccgcaagat aaattttacc aatccctgaa ataaaaatac ttccatctgt
     6541 ttcaacttct ttttgatact cctgttcaaa aaatccccac acattgataa taagtgtatc
     6601 tccaggtcct aaaatataat catcacttaa aggtaactgg tgggttttta aaagtggggt
     6661 gtaattttca aaaaaatcct ctccaaatat ttttaattca ggatatccag aaaaaacttc
     6721 acagtttaat ttaaaagaga aaaaacaaat aataaatgta aaaactaatt ttttcaaaaa
     6781 atgtgaccag tcaattttta ttatcctgta aaatttttca attttcattt catttccttt
     6841 attttaaaca aaaaaactaa caattttaaa aagatgtttg agattattaa aatttatcac
     6901 actcttttat atttttcaag aatatctggt ataatattta aaaattaaaa actcggaaaa
     6961 ttctaaagaa aatggaagaa ataaaggata taaaactgga aacagaagca gaccttgatt
     7021 tattcggcaa tcctgcaaaa attaggattg ttgctacatc tgataaactt tatataattc
     7081 ctgaaaaagg atttcaggaa aaaatttctt ttgatttaaa aaggatagaa tcttttaata
     7141 tacgacagac ggttggaagt gctttttttc aaattaaaat agatggatat tttattgaca
     7201 tatttaggtt cacaaattca aacagatata aattttctcg ttttataacc caggtacaaa
     7261 atttaaaatc agg
//
LOCUS       DAOSAT010000090         7125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_6743_length_7125_cov_4.902546, whole genome shotgun sequence.
ACCESSION   DAOSAT010000090 DAOSAT010000000
VERSION     DAOSAT010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6743_length_7125_cov_4.902546"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="PLF90_07605"
     CDS             complement(<1..1200)
                     /locus_tag="PLF90_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOK57178.1"
                     /translation="MEIKINTELGVFNIFLIFFLFIIIVFILYKTVFLKNNKLIFLLD
                     FFSAILLLSIILQIEITLKIKKREKSNLVILIDTSLSMSIEENGKKRFDTTKEILKKY
                     SKRLKKYKPVFYTFGSSLKKIEEKDIEKLKVQDEETNILKNILKIREINKNNKISGIL
                     LFSDGIETSSTTINDLKNIEIPVYTIVSRKSDLKDISIDRVTGSQYIYKGEKNKIEVF
                     LKSSNFRDKKVKINLKKNNKTLDSKIIEVKEENQKVEFEIISENSGYEIYEIEVISDE
                     KELNNENNRKNFVVMNISPLIKVLYIEGYLRWEYKFLKNFLEGNPNIESISIINIGKQ
                     TYQQTGGKEFNFQGSLFEKKSDFENFHIIILGDIDFSGFSKIQMKNIEEIVNNGSNII
                     FLGGKNFL"
     gene            complement(1203..3182)
                     /locus_tag="PLF90_07610"
     CDS             complement(1203..3182)
                     /locus_tag="PLF90_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOK57179.1"
                     /translation="MINFLNPVFLWSLLGISLPVLIHFFGKRSRKLIPFSYIKILKLT
                     KKQTLKIEKIQEFFIILFRTILIFSILFVLSEPLSNSPIFKKEKKYVVFVIDNSMSML
                     ANSGYGIEKTKKIIYKILNNFDKKTEISLIYLNGKFYNFQNDLSKIKDIIDKENLYYF
                     SGNFRWALMKSEEILRNKEGEKFIFIFSDLQKKSFQDLDLNFKEEHIFIFDVSSENTE
                     NLSIKDVHSSNKKNCFTVDIKNWGNSKKDFYLSVKDNITKKIKEEKLSINPEENKKYE
                     IEFNSESENIIFEINTEDAILADNIYFFTKTKKEKKLLLISEDEKALKFIINGLEALK
                     SFLELNFNVVKMNDIEKIYLPEYQLIILNDTGRIKKEVIKSFNSYLKDTGNIILFIGD
                     RTYPEIFNEIWEIKEEKIYLLPCKIEKTIISEKPLDINYIEKKEPVFYDFGEKIFEYI
                     NPVKFWKYYKIKENHGISLLKIEGNNDIMIKKNIGEGNIILFPFVLKNEWTNFQNKPF
                     FPVFLSKIIENYAKDTNVNYIIGNQKKMNIPKDTEKIEIYKPDGELFKIVLKNENYEF
                     NPDIPGFWKIKFYKEKEKKIAVNVDSSEGNLQKISYKEIKELFHGNLKIINDEKFEKF
                     EKNVLKSRDLTDFFLSTAFIFLFLNVLFSEIMKFR"
     gene            complement(3179..4069)
                     /locus_tag="PLF90_07615"
     CDS             complement(3179..4069)
                     /locus_tag="PLF90_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOK57180.1"
                     /translation="MKVAGYLDPKVLIKFSSLNLVAKKSVEGFLSGLHKSIYKGFSVE
                     FFEHREYAPGDDIRYIDWKVFGRTDKLFLKTYKEETNVRCFILLDTSNSMAYKTGEIS
                     KIQYGVYLSACLSYLILNQKDAVGFVNFNNEIKNFLKASSSFSHFHNIIEILEKIKPE
                     GQTSILSVMKKISSSIKKRSIIILISDLFDNPQDVIKGIAYFTHHHHEVIVFQILDHG
                     EISLKGEGILEFFDIETEEKISVDNEFIKKKYKENIEKLINFYKKRFTEMKIDYKTAI
                     TTKPFDSFLYEYLKLRMRKK"
     gene            complement(4090..5079)
                     /locus_tag="PLF90_07620"
     CDS             complement(4090..5079)
                     /locus_tag="PLF90_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOK57181.1"
                     /translation="MGENLLEKLEELKEIKKEIYEEIHKVIIGQDNVIEKLLISLFTG
                     GHCLLIGVPGLAKTLMIKTLAEILDLTFKRIQFTPDLMPSDITGTEIIQEDRITKERI
                     IKFIQGPIFANIVLADEINRAPPKTQAALLESMQEYQVTVGGKIHLLEMPFIVFATQN
                     PIELEGTYPLPEAQLDRFMLSIKIDYPEENEEKKIAEITTMGITPKLKKILSRERIIE
                     FQNLIKKIPISDFIIDYVVKLVRSTRPGINHNSKFIQDYVSWGAGPRGVQFIVIASKA
                     VTALRGDVNISISDIKKVVKDVLRHRIFLNFNADADGIDTEYIIDKLLEEVKV"
     gene            complement(5083..6513)
                     /locus_tag="PLF90_07625"
     CDS             complement(5083..6513)
                     /locus_tag="PLF90_07625"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOK57182.1"
                     /translation="MKKIYSFILLIFIILCLNIESWECGKAPPTQQPQRRKAGEGFPP
                     LPLPVTPLRRSEKKNPPSPPVIIGKVICGSDDKWTRAENDIENLLKIIGQSTKINYRA
                     VKISLNNFSFDPEEIPILYITSVEPFSLEEKNFTKIKEFLLKGGFIWANASSGSPEFT
                     KEFINIIEKIFPDRNFYPLYSNHPIINCFHNIKNVKILEEGKEKEINLNLRTLNIGCR
                     AAVILSPYDLGCGWAMHTHPWGVRYLPEDAIKIGTNMFLYSLNWIEYGKFYSQLPVYT
                     EKEKSKAGKIYIGQVKHSGDWNPSPNSIGKFLKNFSEKTETFVYLEPISVDLKKDTLK
                     DVPVLYITGHYDPNFDEIELKKLRDFLLKGGCLIGNSCCGSEDFTIAFQKIINKILPE
                     AKKVIPKENDKIFQFPFVINTIFSTYRFEDPIEIYYLKEQPIIVFSKYDIGSGWENIP
                     KPFVFSLNIKESKEIGTNILVYLLTH"
     gene            6601..7068
                     /locus_tag="PLF90_07630"
     CDS             6601..7068
                     /locus_tag="PLF90_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57183.1"
                     /translation="MEEKVIKENLKKIKIAGFLLILYPLLLIFVGKILILKGFTHIVL
                     DYENEKIKVVQNILYFLGLGIFFFCGGFSDFISKKLFSNKKDISEKKQAYFFYTVLMF
                     LFLNIISTCGFIGFLICGNFAWLVTFSLINFLSLFSYFPTQRRYIKKLEMFTD"
BASE COUNT         2336 a          989 c          794 g         3006 t
ORIGIN      
        1 tagaaaattt ttaccaccaa gaaaaattat gttggaaccg ttatttacaa tttcttctat
       61 atttttcatc tgtattttgc taaatcctga aaaatctata tcacctaaaa taataatgtg
      121 gaagttctca aaatcagatt ttttttcaaa taaactcccc tgaaaattaa attcttttcc
      181 tccagtttgt tgataagttt gtttccctat atttattata gagatggact ctatatttgg
      241 atttccctca agaaaatttt ttaaaaattt gtattcccat cttaaatatc cttcaatata
      301 caaaactttt atcagtggtg atatattcat aactacaaaa tttttcctgt tgttttcgtt
      361 atttaattct ttttcatctg aaataacttc aatttcataa atttcataac ctgaattttc
      421 tgaaataatt tcaaattcta ctttttgatt ttcttcttta acctctatga ttttactatc
      481 taatgtttta ttattttttt tcaagtttat ttttactttt ttatctctaa aattagatga
      541 tttaagaaaa acttctattt tatttttttc tcctttatat atatattgag aacccgttac
      601 tctgtctata cttatatctt ttaaatcact tttccttgat acaattgtat aaacaggtat
      661 ttcaatattt ttgagatcat taatagttgt agaagatgtt tctattccat cactaaataa
      721 tagaatacct gaaattttat tatttttatt tatttctctt atttttaata tgttttttaa
      781 aatgtttgtt tcttcatcct gaactttaag tttttctata tctttttctt ctattttttt
      841 tagagaagaa ccaaatgtat aaaaaactgg tttatatttt tttaatctct tgctgtattt
      901 tttcaatatt tcttttgtcg tgtcaaatcg tttttttccg ttttcttcta ttgacatact
      961 taaagatgta tcaatcaata taactaaatt acttttttct ctcttcttaa tttttaaggt
     1021 aatttcaatc tgtaaaatta ttgaaagaag taaaattgcg gaaaaaaaat caagaagaaa
     1081 tattaattta ttatttttta aaaaaactgt tttgtaaagg ataaaaacaa ttattatgaa
     1141 taaaaaaaat attaagaaaa tgttgaaaac tccgagttct gtatttattt ttatttccat
     1201 ttttatctaa atttcattat ttcagaaaaa agtacattta aaaaaagaaa aataaaagca
     1261 gttgaaagga aaaaatctgt taaatctcta ctttttaaaa cattcttttc aaatttttca
     1321 aatttttcat catttataat ttttagatta ccatgaaata actctttgat ttctttataa
     1381 gatatttttt gtaaatttcc ttcactgcta tcaacattta ctgcaatctt tttttctttt
     1441 tctttataaa atttaatttt ccagaaacca ggaatgtcag gattaaattc ataattttca
     1501 ttttttaaaa caattttaaa aagttctcca tcaggtttat aaatctcaat tttttcagta
     1561 tctttaggaa tattcatctt tttctgattt cctatgatat aattcacatt tgtgtctttt
     1621 gcatagtttt caatgatttt acttaaaaaa acagggaaaa atggtttatt ttgaaaatta
     1681 gtccattcat ttttcaaaac aaaaggaaat aaaattatat tcccttctcc aatattcttt
     1741 tttatcataa tgtcattatt tccctctatt ttcagaagtg aaataccatg attttctttt
     1801 attttgtagt atttccagaa ctttacaggg tttatatatt caaatatttt ttcaccaaaa
     1861 tcataaaaaa caggttcttt tttttcaatg taattaatat ccaatggttt ttcacttata
     1921 atagtttttt ctattttaca tggaagtaaa tatatttttt cttcttttat ttcccatatt
     1981 tcattaaaaa tttcaggata agttctatca ccgataaaaa gaattatgtt tccagtatct
     2041 tttaaataag agttaaaaga ttttatcact tcttttttaa ttctgcctgt gtcgtttaaa
     2101 attattaatt gatattctgg taaataaatt ttttcaatgt cattcatttt aactacatta
     2161 aaattaagtt ccaaaaatga ttttaacgct tcaaggccgt ttattatgaa ttttaatgcc
     2221 ttttcatctt cggaaattaa aagaagtttt ttttcctttt ttgtttttgt gaaaaaataa
     2281 atattatccg ctaaaattgc atcttctgta tttatttcaa atataatgtt ttctgattca
     2341 gaattaaatt caatttcata ttttttattt tcttcaggat taatactcaa tttctcttct
     2401 tttatttttt ttgtaatatt atcctttaca gataaataaa aatccttttt tgaatttccc
     2461 cagtttttaa tatcaactgt aaaacaattt tttttatttg aagaatggac atcttttatt
     2521 gataaatttt ctgtattttc agatgaaaca tcaaaaataa aaatatgttc ttctttaaag
     2581 tttaaatcca gatcttggaa tgattttttc tgtaggtcag aaaaaataaa tataaatttt
     2641 tcaccttctt tattccttaa tatttcttca cttttcataa gtgcccatct aaaatttcct
     2701 gaaaaataat ataaattttc tttatctata atatctttta ttttagaaag gtcattttgg
     2761 aaattgtaaa atttaccatt taagtaaatc agagaaattt ctgttttttt atcaaagtta
     2821 tttaaaattt tgtagataat ttttttggtt ttttcgatac cataaccact gtttgcaagc
     2881 atactcatag agttatcaat tacaaagacc acatattttt tttctttttt aaaaattgga
     2941 gaattactca acggttctga taaaacaaaa agaatagaaa aaattaaaat agttctgaat
     3001 aaaattataa aaaattcctg tattttctca attttgagtg tttgtttttt ggttaatttt
     3061 aaaattttta tataactaaa aggaattagt tttctacttc tttttccaaa aaaatgtatt
     3121 aatacaggta aacttattcc caaaagtgac catagaaaaa caggatttaa aaaatttatc
     3181 atttttttct cattcttaat ttcaagtatt cgtaaagaaa actatcaaaa ggtttggttg
     3241 ttattgctgt tttataatca attttcattt ctgtaaatct ttttttataa aagtttataa
     3301 gtttttcaat attttcttta tatttctttt ttataaattc attatcaact gaaatttttt
     3361 cttctgtttc aatatcaaaa aattccaaaa ttccttctcc tttcaaagaa atttctccgt
     3421 ggtcaagtat ttgaaagaca attacttcat gatggtgatg agtaaaatat gcaattcctt
     3481 ttattacatc ttggggatta tcaaaaaggt cagaaattaa aatgattata cttctttttt
     3541 ttatacttga ggatattttt ttcataacag aaaggattga tgtctgacct tccggtttta
     3601 tcttttcaag aatctcaata atattatgaa aatgagaaaa ggaagaagat gcttttaaaa
     3661 aattttttat ttcgttattg aaattaacaa atccaacagc atctttttga tttaatataa
     3721 gataagaaag acaagcagat aaatatactc catattgaat tttactaatt tcaccagttt
     3781 tatatgccat tgaattactt gtatcaagta atataaaaca tcttacattt gtttcttctt
     3841 tatatgtttt taaaaataat ttatctgttc taccaaaaac tttccagtca atatatcgta
     3901 tatcatctcc aggtgcatat tctctgtgtt caaaaaattc aacactgaat cctttataga
     3961 tacttttatg aagtcctgaa agaaatcctt caacactctt tttagctacc aaatttaaac
     4021 tggaaaattt aataagaact tttggatcaa gatatcctgc aactttcatt gtaatagtta
     4081 agagattttt taaactttaa cttcttcaag caatttatct ataatatatt cagtatctat
     4141 accatcagca tctgcattaa agtttagaaa aattctgtgt cttaaaacat ctttgacaac
     4201 tttttttata tctgatattg atatattgac atctcccctt agtgcagtta ctgcttttga
     4261 ggcaatgacg ataaattgaa cacctcttgg tcctgctccc caacttacgt aatcctgaat
     4321 gaattttgag ttatgattga tacctggtct tgtacttcta acaagtttta ctacatagtc
     4381 aataataaaa tcagaaattg gtattttttt tattaagttt tgaaattcaa ttatcctttc
     4441 tctactcaat atttttttta attttggagt aattcccata gtggttattt ctgcaatttt
     4501 tttttcttca ttttcttcag gataatcaat ttttatgctt aacataaatc tatccagttg
     4561 ggcttctggt aaaggataag ttccctccag ttcaatcgga ttctgtgttg caaagacaat
     4621 aaaaggcatt tctaaaagat gaattttccc tccaacagta acctgatatt cctgcattga
     4681 ttcaagaagt gctgcctgtg ttttgggtgg agctctattt atttcatctg caagtactat
     4741 atttgcgaaa attggtccct gaataaattt aattattctt tcttttgtaa ttctgtcttc
     4801 ctgaattatt tctgttcctg taatatcaga tggcattaaa tcgggtgtaa attgtattct
     4861 tttgaatgtt aagtcaagta tttctgcaag tgtttttatc ataagagttt ttgcaagtcc
     4921 gggaacgcca attaatagac agtgtcctcc tgtaaataaa gaaattagca atttttcaat
     4981 tacattatcc tgtcctataa taactttgtg gatttcttca tatatttctt tttttatttc
     5041 ttttaactct tcaagttttt caagtaaatt ttcacccatt ttttagtgag ttaataaata
     5101 aactagaata ttcgttccta tttcttttga ttctttaata tttaaagaaa aaacaaaagg
     5161 tttcggtata ttttcccatc cacttccaat atcatattta gaaaaaacaa ttattggttg
     5221 ttcttttaaa taatatattt caattgggtc ttcaaatcta taggtagaaa agattgtatt
     5281 tataacaaaa gggaattgaa aaattttatc attttctttt ggaattactt tttttgcttc
     5341 tggaagtatt ttatttatta ttttttgaaa tgcaattgta aagtcctcac ttccacagca
     5401 actattacct atcagacaac ctcctttcaa aagaaaatct cttaattttt tcaattctat
     5461 ttcatcaaaa ttcgggtcat aatggcctgt aatgtaaagg acaggtacat cttttaaagt
     5521 atcttttttc aaatcaacag aaattggttc taaataaaca aatgtctctg tcttttctga
     5581 aaaattttta aggaattttc caattgaatt tggggaagga ttccaatcac cagaatgttt
     5641 aacctgtcct atataaatct tccctgcttt acttttttct ttttccgtat atactggtaa
     5701 ctggctataa aattttccat attcaatcca gttcagagaa tataaaaaca tatttgttcc
     5761 tattttaatt gcatcttccg gtaaatatct tactccccat ggatgagtat gcattgccca
     5821 tccacatcca aggtcataag gggacaaaat tacagcagct ctacatccaa tattcaatgt
     5881 tcttaaatta agatttattt ctttttcttt accttcctct aaaattttta catttttaat
     5941 attatgaaaa cagtttataa tagggtgatt tgaataaagt gggtaaaaat ttctatcagg
     6001 gaatattttt tcaattatat ttataaattc ttttgtaaat tcaggacttc ctgaagaagc
     6061 atttgcccag atgaaaccac cttttaataa aaattctttg atttttgtga aatttttttc
     6121 ttcaagtgaa aatggttcaa cacttgttat gtaaagaatg ggtatttctt ccgggtcaaa
     6181 ggaaaaatta tttaaagaaa tttttactgc tctataattt atttttgtag attgtccaat
     6241 tattttaaga agattttcaa tgtcattttc tgctcttgtc catttatcat cggaaccaca
     6301 aataactttt cctattatta caggtgggga tggaggattt ttcttttcgc ttcttcttaa
     6361 aggtgttaca ggtaaaggta aaggtggaaa tccttctcct gcttttcttc tttgaggttg
     6421 ttgtgtgggt ggggctttac cacattccca actttcaata tttaaacaca aaattataaa
     6481 gattaataga ataaaactgt atattttttt catttttcgt ctaaataaat ctactctaaa
     6541 agttattata tcaaaaataa ttttgaaaag tcaaaatttt ataataaaat aaataccatt
     6601 atggaagaaa aagtcattaa agaaaacctt aaaaaaataa aaattgcggg gtttctttta
     6661 attttatatc ctttgcttct tatatttgta ggtaaaattt taatcttaaa aggttttaca
     6721 catattgtat tggattatga aaatgaaaaa attaaggttg ttcaaaatat cctttatttt
     6781 ttaggacttg gaattttctt tttctgtgga ggtttttctg attttatatc aaaaaaatta
     6841 ttctccaata aaaaagatat ttctgaaaaa aaacaagcat actttttcta tacagtttta
     6901 atgttccttt tcttaaatat catttctacc tgtggattta ttggattttt gatatgtggg
     6961 aattttgcat ggcttgtaac tttttctcta attaattttt tatctctatt ttcatatttt
     7021 cctactcaga gaagatatat aaaaaaactg gaaatgttta cagattaaaa agagcgggat
     7081 acgggatttg aacccgcgac cacgagcttg ggaagctcgc actct
//
LOCUS       DAOSAT010000091         7088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_6797_length_7088_cov_4.811460, whole genome shotgun sequence.
ACCESSION   DAOSAT010000091 DAOSAT010000000
VERSION     DAOSAT010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6797_length_7088_cov_4.811460"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..353)
                     /locus_tag="PLF90_07635"
     CDS             complement(54..353)
                     /locus_tag="PLF90_07635"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOK57184.1"
                     /translation="MHKINILGKNYTIHSSFSSNEINKIAKEIDEKYKTFEKEYKTFD
                     KIDILIFYLIELYEVIHKLEKRLSKEEEIKNKIKNKIIEIEKDIITTLKNLTIIE"
     gene            complement(346..2334)
                     /gene="pheT"
                     /locus_tag="PLF90_07640"
     CDS             complement(346..2334)
                     /gene="pheT"
                     /locus_tag="PLF90_07640"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOK57185.1"
                     /translation="MKYTYKTLMNYIDGEIKKDDFINYLTILGLNPKIIREYKDDFIF
                     ELETPANRPDLLSFIGIIKEIIPFGNIFLKNLREEITEEITDFIPIEIENENDCPYYS
                     CRIVKNIDNKLSSGDFRKIIENIGFRPSLQVVDISNLVMCETGQPLHIFDFDKIKGKI
                     IVRKGKKGEKIITIDGKERDVEDILVIADEEKPIAIAGIMGGMNTEVTYNTRNILIES
                     AYFNPVLIRKGSKKLGLVTEASLRFEKGLSVSMAKNGMERVTIMIKKHCGGKIGKHNF
                     AGNQIISENKIYVDKEKVEKILGIQIEKEFLKNLFSKMDFSIEEKNNILLLKIPETRK
                     DLKEEIDIIEEIAKYRKYSDIPSEMPVVNINPFSLNSSYELVKNMKNILVNLGFMETI
                     TLSFISDEVVKKFNLNAIKIENPLSQMFSFLRNSLIFNLFEVLKYNISHQNKKIEIFE
                     FGKIFKREENRYLELQSITLISLNSGTFFDFKGKIEHFLEKCGLKEVSFIKNNFFLAQ
                     EEANCDIYFFDDKIGNLFIPIDELKNFYGIETEVYVCEILLDKLLQYSNFEKKFKQLP
                     KFPFSQRDFSFIISENVNWKEIESEIKKLNLPIEKIELFDVYSGKNIPEGKISISFSI
                     IFRLHEKTLENYEVDNFEKSIIDKIEKKFNAQLRGKNA"
     gene            complement(2337..3353)
                     /gene="pheS"
                     /locus_tag="PLF90_07645"
     CDS             complement(2337..3353)
                     /gene="pheS"
                     /locus_tag="PLF90_07645"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOK57186.1"
                     /translation="MVEKIIENAEKFFEEGKREIEGINESDLLEKWKIKFLGRKSYLN
                     QLFSQIPKLSEEEKVICGQKLNEIKKKLVELYETGKLKVKKREIKYNLTFPGKKITTG
                     NLHPITKVIDEIVQIFYKIGFGVAEGPEIETDYYNFTALNTPKEHPARDIWDTFYIEK
                     ENNILLRTHTSPVQVRIMEKYSPPLRIIAPGKCFRRDAFDSSHSPMFHQIEGLMVDKN
                     IKFTHLKGVLSYFIHEFFKKEVKVVFSPSYFPFTEPSAEVSISCVICGGEGCSTCGNT
                     GFLEILGCGMVHPNVFKNVGYDPETFTGFAFGMGVERLAMLKYGIDDIRYFFFNDLRF
                     LDQF"
     gene            complement(3334..3942)
                     /gene="nadD"
                     /locus_tag="PLF90_07650"
     CDS             complement(3334..3942)
                     /gene="nadD"
                     /locus_tag="PLF90_07650"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOK57187.1"
                     /translation="MEMDRSENKKIGIFGGTFDPIHYGHLIIAEKAREEFELDFIIFI
                     PAGIPPHKKKVYASPIDRKNMIEIAIKDNKYFKVSDIEILKKEKSYTYETVIWLKKEY
                     PNSDFFLIVGEDSFYDIPNWYKANELVKEVIFLVARRSNKIDISTNFQINYRIINSPF
                     IDISSTYIRSCIFENKSVKYLIPDEVIDYIKKKGLYHGRENY"
     gene            complement(3917..5173)
                     /locus_tag="PLF90_07655"
     CDS             complement(3917..5173)
                     /locus_tag="PLF90_07655"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOK57188.1"
                     /translation="MEWKDRLIETVKSTKEASFEIAKITEKEKNIFLEILSEKILQKK
                     DEIIKENEKDVRNCIRRNYSNAFIDRLKIDEKRINKMVKSVKDVISLPDPVGQKIWET
                     IRPNGLKIEKIRVPIGVIGIIYESRPDVTVEASILCIKSGNSVILRGGKEAKNTNLKL
                     VEIIKESLRESEMSENIVNLITTGGRKSVKYLLKLYQYIDLIIPRGGESLIKTVVENS
                     YIPVIKHYAGVCHTYVDKDADLEMAWKVVLNAKVQRPATCNATETLLVHKDIAKKFLP
                     KMAEIFKEYKVEMRGCEKTLEILPDIKRATEIDWYTEYLDLIISIKIVKNLEEAIEHI
                     NKYGTHHSDSIITENEKAAEKFLKEVDSAAVYHNASTRFTDGGEFGLGAEIGISTDKI
                     HARGPMGLEELTTYKYIIYGNGQIRE"
     gene            complement(5152..5979)
                     /gene="proB"
                     /locus_tag="PLF90_07660"
     CDS             complement(5152..5979)
                     /gene="proB"
                     /locus_tag="PLF90_07660"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOK57189.1"
                     /translation="MKKNKVFSNLKRIVIKIGTNVLTTESNRLDTSIIEHIVEQISYL
                     VKKENKEIIIVTSGAIAAGMQILGWEKRPKKISMLQAAASVGQSRLMRTYERLFREEG
                     LNVGQILLTRDVLTIPERRVYARNTLLTLLKYKVIPIINENDSVAVEEIKFGDNDILS
                     SYVVDLIDADILIILSDVDGVFTNDPKRNKGKIIREIIEINEIENKIKIGLPSKKGTG
                     GIKSKIEAAKFVTANNKYCVILNGKKINCIKKLFSGEKIGTIFYPLKRSKKWSGKTD"
     gene            complement(5976..6824)
                     /locus_tag="PLF90_07665"
     CDS             complement(5976..6824)
                     /locus_tag="PLF90_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOK57190.1"
                     /translation="MSEFKNIGIIGLGLIGGSLGLDLKKKKIAEKIIGYSRKKETMEK
                     AVQIRAIDMYCKNPEELIEKVDFLILATPIRTIEEYIKLIKKVKPEIFFTDVASVKKI
                     ICEKVKKIIGEKTNFVGSHPISGSEKSGIDFIKEGLFENKTAVITITEETNKIAKEKT
                     KKIWKSLGSKVFEMSPEEHDRILGFTSHLPHFLVYSLLATAQKNKNILKDFFGSGFLD
                     TTRIGKSNPQMWSEIFLYNKKNILLWIKKFEKEMKKLKKLIENNNIEDIKKYLTKSKN
                     FREDLE"
     gene            complement(6831..>7088)
                     /locus_tag="PLF90_07670"
     CDS             complement(6831..>7088)
                     /locus_tag="PLF90_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOK57191.1"
                     /translation="TMKRAMARQAEVERERRAKIINAEGEFQAAQKLKEAAEILSEVP
                     MAIQLRYLQTATEIAAEKNSTLVFPLPLEIFKHFIKKEEEK"
BASE COUNT         2138 a         1174 c          754 g         3022 t
ORIGIN      
        1 gggcctggcg agctaccact gctccacccc gcaatgaaat ttatattacc aacttattca
       61 atgattgtca agtttttcaa tgttgtaata atatcttttt ctatctctat tatcttattt
      121 tttattttgt tcttaatttc ttcttctttt gacaatcttt tttctaattt atgaataacc
      181 tcgtaaagtt ctataagata aaaaataagt atgtcaattt tatcaaatgt tttatattct
      241 ttttcaaatg ttttatattt ttcatctatt tctttagcaa ttttgttaat ctcatttgaa
      301 gaaaaagaag agtgaattgt ataattttta ccaagaatgt ttattttatg catttttacc
      361 tctcaattgt gcattaaatt ttttttcaat tttatcaatt atgctttttt caaaattatc
      421 gacttcgtaa ttttcaagtg ttttttcatg taaacgaaat attattgaga aacttatact
      481 tattttacct tcaggaatgt ttttaccact ataaacatca aagagttcaa ttttttctat
      541 aggtagattt aattttttta tttcactttc gatttccttc cagttaacat tttcagaaat
      601 aataaatgag aaatctctct gagaaaaagg gaatttaggt aattgtttaa attttttttc
      661 aaagtttgaa tattgtaaaa gtttatccag taaaatttca cagacataaa cttcagtttc
      721 tattccataa aagtttttca attcatcaat gggtataaat aaatttccta ttttatcatc
      781 aaaaaagtaa atatcacagt ttgcttcctc ctgtgctaaa aaaaagttat tttttataaa
      841 actcacttct ttcaagccac atttttcaag aaaatgttca atttttcctt taaaatcaaa
      901 aaaagttcca ctgtttaatg aaatgagtgt aatagattgt aattctaagt atctattttc
      961 ttccctttta aaaattttcc cgaattcaaa aatctcaatt tttttgtttt gatgtgaaat
     1021 attatatttt agtacttcaa aaagattaaa aattaaagaa tttctcaaaa aagaaaacat
     1081 ttgtgataat ggattttcta tttttattgc gttaagattg aattttttaa caacttcatc
     1141 agaaataaaa ctcaaagtta ttgtttccat gaatccaaga ttaacaagaa tatttttcat
     1201 gttttttact aattcataac ttgaatttaa tgaaaaagga ttaatattaa ccacaggcat
     1261 ttctgaagga atatctgaat attttctata ttttgcaatc tcttctataa tatcaatttc
     1321 ttcttttaaa tctttcctgg tttccggtat ttttaaaagt aaaatgttat ttttttcttc
     1381 tattgaaaaa tccattttac taaataaatt ttttaaaaat tctttttcta tctgtattcc
     1441 aaggattttt tctacttttt ccttatcaac atatatttta ttttcagaaa taatctgatt
     1501 tccggcaaaa ttatgttttc ctattttccc tccacagtgt ttttttatca ttatggttac
     1561 tctttccatt ccatttttag ccatagaaac gcttaatccc ttttcaaatc tcaaagatgc
     1621 ttcagtaaca agtcctaatt ttttactacc ttttcttatt aaaacaggat taaaatatgc
     1681 actttctata agaatgtttc ttgtattata tgttacttct gtgttcatcc ctcccataat
     1741 tcctgcaata gcaattggtt tttcttcatc tgcaataact aaaatgtctt ctacatctct
     1801 ttctttacca tctatggtta ttattttttc tcctttttta ccttttctaa caattatttt
     1861 tccttttatc ttatcaaaat caaaaatatg taatggttgt cctgtctcac acattactaa
     1921 attggaaata tcaacaactt gaagagaagg tctgaaacca atattttcta ttatttttct
     1981 gaaatctcct gaagaaagtt tgttatcaat gttttttact attctacagg aataataagg
     2041 acaatcattt tcattttcaa tttcaattgg gataaagtcc gtaatttctt cggttatctc
     2101 ttcccttaaa ttttttaaaa aaatatttcc aaaaggaatg atttctttta taataccaat
     2161 aaaacttaaa aggtccggtc tatttgcagg tgtttcaagt tcaaatataa agtcatcttt
     2221 atattctctt attattttag gattcaaacc aagtatagtt aaataattta taaagtcatc
     2281 tttctttatt tccccatcaa tataattcat taaagtttta tatgtatatt tcatttttaa
     2341 aattggtcta aaaatcttaa atcattgaaa aaaaagtatc ttatatcatc tattccatat
     2401 tttaacatgg caagccgttc aactcccatt ccgaaagcaa aacctgtaaa agtttcaggg
     2461 tcatatccaa catttttgaa aacattagga tgtaccattc cacatccaag gatttcaaga
     2521 aaaccggtat ttccacaagt ggaacatcct tccccaccac atatcacaca tgaaatacta
     2581 acttctgcac ttggttctgt aaagggaaaa tatgaaggag aaaaaactac ttttacttct
     2641 tttttgaaaa attcatgaat aaaataagac aggactcctt ttaaatgtgt aaatttaata
     2701 tttttatcaa ccataagtcc ttcaatttga tgaaacatag gactatgaga agaatcaaaa
     2761 gcatctcttc taaaacattt accgggagca attattctta aaggtggaga gtatttttcc
     2821 ataatcctta cctgaacagg acttgtgtgg gttcttaaaa gtatattatt ttctttttct
     2881 atatagaatg tatcccagat atcccttgct ggatgttctt tcggagtatt taaggcagta
     2941 aaattataat aatcagtttc aatttctggt ccttccgcaa caccaaaacc tattttataa
     3001 aaaatttgaa ctatttcgtc tataaccttt gttattggat gtaaatttcc tgttgttatt
     3061 ttttttcctg ggaaagtaag attatatttt atttctctct tttttacctt taacttgcct
     3121 gtttcatata gttccaccaa tttctttttt atttcattta gtttttgccc acatattacc
     3181 ttttcttctt ctgatagttt tgggatttgt gaaaaaagtt gattcaaata tgactttctc
     3241 ccgagaaatt ttatcttcca cttttccagt aagtcacttt catttattcc ctctatttct
     3301 ctttttcctt cttcaaaaaa cttttccgca ttttcaataa ttttctctac catgatataa
     3361 acctttcttt tttatataat ctataacttc atcaggaatt aaatatttta ctgatttatt
     3421 ttcaaaaatg caactcctta tgtaagtaga ggaaatatca ataaaaggag aattgataat
     3481 tctataattt atttgaaaat ttgttgaaat atctattttg ttacttcttc ttgctacaag
     3541 gaatataact tctttaacaa gttcatttgc tttataccag tttggaatat cgtaaaaaga
     3601 gtcctctcca acaatcaaaa aaaaatcaga atttggatat tcttttttca accaaataac
     3661 tgtttcataa gtataacttt tctctttttt taatatttca atatcagaaa ctttgaaata
     3721 tttattatcc tttatcgcta tttctatcat gttttttcta tctatgggag aagcataaac
     3781 ttttttttta tgaggtggaa ttccggcagg gataaaaata ataaagtcaa gttcaaattc
     3841 ttctcttgcc ttttctgcta taattaaatg tccataatga attgggtcaa aagttccacc
     3901 aaaaattcct atttttttat tctctgatct gtccatttcc atatattata tatttgtaag
     3961 ttgttaactc ttctaatccc attggacctc ttgcatgtat tttatctgta cttattccaa
     4021 tttctgctcc aagaccgaat tcccctccat ctgtaaatct tgttgaggca ttgtgatata
     4081 ctgctgctga atcaacttct tttaaaaatt tctctgctgc tttttcattt tcggtaatta
     4141 ttgaatcaga atgatgtgtt ccatatttgt ttatatgttc aatcgcttct tccagatttt
     4201 tcacaatttt tattgagatt ataaggtcaa gatattctgt ataccagtca atttctgttg
     4261 ctcttttaat atcaggtaaa atctctaatg ttttttcaca tcccctcatt tcaactttat
     4321 attctttaaa aatttctgcc atctttggta gaaatttttt tgctatatct ttatgaacaa
     4381 gaagtgtttc tgttgcatta catgttgcgg gtctttgtac ttttgcattt aagacaactt
     4441 tccatgccat ttctaaatct gcatctttat caacataagt atgacagaca cccgcatagt
     4501 gttttattac agggatataa ctattttcta cgaccgtctt tattaatgat tctcctcccc
     4561 ttggaattat aaggtcaata tattgataga gtttaagtaa atattttaca gattttcttc
     4621 cgccggttgt tattaagttt acgatatttt cagacatctc agattctctt aatgattctt
     4681 tgattatctc cacaagtttt aaatttgtat tttttgcctc tttaccacct cttaaaatta
     4741 cagaattacc tgatttaata cataaaatac ttgcttcaac agtgacatca ggtcttgatt
     4801 cataaataat tcctataact ccaatcggta ctctaatttt ttctattttt aatccattcg
     4861 gtcttattgt ttcccatatt ttttgaccaa cagggtcggg aagagaaatt acatccttta
     4921 ctgatttaac cattttattt attctttttt catcaatttt taatctatca ataaaagcat
     4981 ttgaataatt tctccttata caatttctaa catccttttc attttctttt attatttcat
     5041 cttttttttg tagaattttt tcagataata tttccagaaa aatatttttt tctttttcag
     5101 taatttttgc gatttcaaaa gatgcttctt ttgttgattt tactgtttca attaatctgt
     5161 ctttccactc cattttttac tccgttttaa aggataaaaa attgtaccta ttttttctcc
     5221 actaaaaagt ttctttatgc aatttatttt ctttccattc aaaatgacac agtatttatt
     5281 atttgctgta acaaatttag ccgcctctat cttactttta attcctcctg tacccttttt
     5341 tgatggtaaa ccaattttta ttttgttttc tatttcattt atttctataa tttctcttat
     5401 aattttccct ttatttctct ttgggtcatt agtaaaaaca ccatccacat ctgaaagtat
     5461 tatcaatata tcagcatcaa tcaaatctac aacataactt gacagaatat cattatcacc
     5521 gaattttatt tcttcaactg caacactatc attttcgttt ataataggta taactttata
     5581 tttgagtaaa gttaaaagtg tatttctggc ataaactctt ctttctggaa ttgtaagcac
     5641 atcccttgtt agaagtatct gtcctacatt taaaccttct tccctgaata atctttcata
     5701 agttctcata agacgactct gaccaacact tgctgcagcc tgtaacatac tgattttttt
     5761 tggtcttttc tcccaaccaa ggatttgcat tccagcagct attgctcctg aagtgactat
     5821 gataatttct ttattttctt tttttactaa ataacttatc tgttcaacaa tatgctcaat
     5881 tatagaagta tcaagacgat tactttcagt tgttaaaaca tttgttccaa tttttattac
     5941 aattcttttt aaatttgaaa aaaccttatt ttttttcatt ctaaatcttc cctgaaattt
     6001 ttacttttgg ttaaatattt ttttatatcc tctatgttgt tattttctat tagttttttt
     6061 aattttttca tttctttttc aaattttttt atccagagta aaatattttt tttattatac
     6121 aaaaagattt ccgaccacat ttgaggatta ctttttccta ttcttgttgt atcaagaaaa
     6181 cctgaaccaa aaaaatcctt taaaatattt ttattttttt gagcagttgc aagaagggaa
     6241 taaactaaaa aatgaggtaa atgactggta aaaccaagaa ttctatcatg ttcttctgga
     6301 ctcatctcaa aaactttact tcctaaactt ttccatattt tttttgtttt ttcttttgca
     6361 attttattgg tttcttcagt aattgtaatc acggctgttt tattttcaaa tagaccttct
     6421 tttatgaaat ctataccgct tttttcagaa ccagatattg gatgggaacc aacaaaattt
     6481 gtcttttctc caataatttt tttaactttt tcacaaataa ttttttttac acttgccaca
     6541 tcagtaaaaa aaatttcagg tttaactttt tttatcagtt taatatattc ttctatagtt
     6601 cttattggag ttgcaagaat aagaaaatca actttttcaa ttaattcttc gggattttta
     6661 cagtacatat caattgctct aatttgtact gctttttcca ttgtttcctt ttttctgcta
     6721 tatccaataa ttttttctgc tatttttttc ttttttaagt caagtcccag ggaaccacct
     6781 atcaagccaa gtccaataat tccaatattt ttaaattcag acatttttaa ttatttctct
     6841 tcttcttttt ttataaaatg cttaaatatt tcaagaggta aagggaaaac gagagttgaa
     6901 tttttttctg ctgcaatttc tgtggctgtt tgtaaatatc ttaattgaat agccattgga
     6961 acttctgata aaatttctgc tgcttcttta agtttttgtg ctgcctgaaa ctctccttct
     7021 gcatttataa tttttgctct tctttctctt tcaacttctg cctgtcttgc cattgctctt
     7081 ttcatagt
//
LOCUS       DAOSAT010000092         7080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_6809_length_7080_cov_4.728114, whole genome shotgun sequence.
ACCESSION   DAOSAT010000092 DAOSAT010000000
VERSION     DAOSAT010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6809_length_7080_cov_4.728114"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLF90_07675"
     CDS             complement(<1..514)
                     /locus_tag="PLF90_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOK57192.1"
                     /translation="MKNKIPFEAIINEASAGTGKTYSIIKDILKLKEYYGSYKFLNKI
                     IGITFSENAAIELKERLIKEIFDREYERLSDEEKIELENILLRLNFSTIHSFAKKILK
                     RFAFILHIDPFFKIIEEKESDILFSEALSKTFLDSEETNIFYKILKELKLNQFSQVVS
                     EMKKLHPYVFL"
     gene            complement(504..3170)
                     /locus_tag="PLF90_07680"
     CDS             complement(504..3170)
                     /locus_tag="PLF90_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOK57193.1"
                     /translation="MKKKIFLLPLNLKNKTKFLLSQIDKLNLANNFIYITSNFCKVQD
                     FKLNFYNYFKNSALPFSFTLKSFASKIISEKSSKKIISDVERFLIIIDIIKKEKKEKF
                     YGYTDEGFAKIVLNFIKELKISSIQKVKLSEVKNIISGFPFKFENHKKNIELALNLFE
                     EYENYLYKRNLIDIEDIYDEATNHLTENYESIIFENIVEIPNYQRNFIAKLVKSTESV
                     IFSYYQPDCFSIDTQKLIINDTFNFLKKIINWEIEKIDGEKYEPEIECFNYPTKEEEI
                     KGIINLIEREFKNNKEIGLDDFLITCPDMLNYRNYIKRIFSRFNLPVELIPGYSFIIE
                     PSISPLLEFFTLAETYEWNTLMNILTSPYFSKINQNEVENFSEKIREEYENIGFYKEN
                     FDFEKINYKSIEILKECTNLIKKEMTISEWKDTLEKIIEMTGWHTSEPEIKIEFEKLI
                     DKMNGSYLLKKEEFINLLKKLLEMIEIEEGKGMGIRVSGIMESLGIEKKICFFCGATE
                     ENFPNAPKIEEFLLPDKVKKELGLEYFEKRIARDRCDFYRIRNEHEKVIFTFPSRVEN
                     QLQMKSIFIFDISPKEEITKEFSIDSKKIFDIKIDISKFKKKYIQNGKLNIGVSDLEL
                     LLKCPYKFYLQKVEEIKPYRIPEIREIPDMWGKIIHESIKETFEKEKYINVEKIKEYK
                     DIFETLLNKKADEYLKKNKISSIYRNIVKLRQIEVLKKFEKIIKNFSGMVLLDFEKDI
                     KFENEKLIKDLIIKGRIDILAKMDDKLTIIDIKTGTSWNCSYTENDFFKQCNIQIPLY
                     AWIYSTTNNISYKNIKGMVCNFSFIENEKESEIITIYDFSTKKFNYIEKISEFLNDIK
                     EEILQDKFSFLKVNNESECFICNYKELCIYEK"
     gene            3229..4149
                     /locus_tag="PLF90_07685"
     CDS             3229..4149
                     /locus_tag="PLF90_07685"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOK57194.1"
                     /translation="MKFKKIVRNFLKPFENGIIIYTFLRVLTIFIQVIPVFLCEKIII
                     FLGNFAFFVMKGHRKLALKNMDIAFGDKYTKKEKIKMCKRLFIECMSFLEIFQLYKFK
                     DEELMKMAEIEGEEILKQVVKEKKGVIAVCPHMGNFPFALVILTKKGYPVNMIVARSV
                     NVYTDRDLRKLREKFGVPSISRKDLKKAIEDSQRWIKNGGILCIYLDQHAPNGIKCEF
                     FGKEVYVPAGAAVFARKYKTNVIGLYTVRTGPMKHKIVIEGPYKLKESRNVSEDVKEN
                     TAFFIKRVQNWVEKYPEQWSSWLNPGRFPD"
     gene            complement(4123..5052)
                     /locus_tag="PLF90_07690"
     CDS             complement(4123..5052)
                     /locus_tag="PLF90_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOK57195.1"
                     /translation="MKKGIALCGGGAKSFCQIGVLKVFEKEGFDFEVITGTSMGALIG
                     TIYSFTKSAEKLEEIIFKIFSIRELQKIEKLFSRKRKRSYLYRFIDGIKDFSLLLFDS
                     FKSGLIESEIIKEKIYKYVGKEIYFEEQKNTLGIIATEYFTGRPVIFNSGKIIPSLIA
                     SCAIPGLVTPVKISDNYYVDGGVTSNLPVIANHILGGEVIIGIENNSLLKNHKPLNAF
                     EVFVQIEKIKNHYSNILESSLSDFVLKIELSSVEWFNFSKLKECIKIGQDYALKNLKK
                     IEKIINNPERINLKKEIVENLKDFFLIRESTGI"
     gene            complement(5049..5609)
                     /locus_tag="PLF90_07695"
     CDS             complement(5049..5609)
                     /locus_tag="PLF90_07695"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOK57196.1"
                     /translation="MYRIFMGSIIPSVYLFTNSLFFSLIICSFFLTLLFSLEFERWKN
                     PGVWDYVLRKYGRIFKTPPGKLTGDTYFMLATFIILLFFKKDIAISSLFFLIFGDAGS
                     GILGSKYGKTKIFPGKTLEGFIGGIIFNLIIAFFLHNFLNLPFYILLSGVLISSIVEI
                     LPLKIDDNLTVGIITAFFMNILNNLI"
     gene            complement(5602..6213)
                     /locus_tag="PLF90_07700"
     CDS             complement(5602..6213)
                     /locus_tag="PLF90_07700"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOK57197.1"
                     /translation="MKVIIFVIFSYFVGSISFAYLFTYFLTGQDIRTIGTGNPGAANV
                     ARTLGKKWGIIVWLADTIKGVFPMFLANKYGITNFILLTLIGSSAVIGHCYSIFLKFK
                     GGKGAATSGGIILYLMPKLFPLVIALWFFAQKINPRSPKILTSCILIFFIFLFIFYKT
                     YFFQLFISTLILILVGAIINKDVIQEIKNTKRIKNGNLEKKNV"
     gene            complement(6314..>7080)
                     /locus_tag="PLF90_07705"
     CDS             complement(6314..>7080)
                     /locus_tag="PLF90_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK57198.1"
                     /translation="CVPPFAHIGQEEECIERSIPFFVEKPIHLNLEKAEEIAKKIENK
                     KLITSVGYVLRYFDIIEKMKKLTETEKIGIVRGRYYGEVPGAGRKRWLIKKDMSGGQL
                     IEQATHTVDLMRYFCGDVEEIYAYKFEGINNKIFKEYDVEDGLTMILKFKNGIIGNLT
                     CTWLWKGFESDIEIIGKDVIFFYKGNTIEINKGDRSEKYISNVDPMYQEDFAFIKSVW
                     EKNPELIKSDYKDALKTLKITLKAHDSILKNQPVKL"
BASE COUNT         2468 a         1002 c          815 g         2795 t
ORIGIN      
        1 ccaaaaaaac atatgggtgt aattttttca tttcagaaac aacttgggaa aattgattca
       61 gttttaattc ttttaaaatt ttataaaaaa tatttgtttc ttcagaatct aaaaatgttt
      121 tacttaatgc ctctgaaaaa agtatgtcac tctctttttc ttcaattatc ttaaaaaaag
      181 ggtctatatg caagataaag gcaaatcttt ttaaaatttt ttttgcaaaa gaatggatgg
      241 tagagaaatt taatcttaga agtatatttt caagttcaat tttttcctca tctgataatc
      301 tttcatattc tctatcaaat atttctttaa tcaatctttc ctttaattct atcgctgcat
      361 tctcagaaaa agtaattcct atgattttat ttaaaaattt ataagaacca taatattctt
      421 tcagtttaag aatatctttt attatagagt aggtttttcc tgttcctgca gacgcttcat
      481 tgataattgc ttcaaatggt attttatttt tcatatatac ataactcctt ataattacat
      541 ataaaacatt ctgattcatt atttactttc aaaaaagaga acttatcctg taatatttct
      601 tcctttatat catttaaaaa ttcacttatt ttttcaatat aattgaattt ctttgttgaa
      661 aaatcataga ttgtgattat ttcactttct ttttcatttt caataaaact aaaattacat
      721 accatacctt tgatattctt ataagaaatg ttgttggtgg ttgaatatat ccatgcgtat
      781 aatggtatct gaatattaca ttgtttgaaa aaatcatttt ctgtatagga acaattccag
      841 gaagtacccg tttttatatc aattattgtg agtttatcat ccatctttgc aagtatatct
      901 attcttcctt taataatcaa atcctttatt aatttttcat tttcaaattt aatatctttt
      961 tcaaaatcca gtaaaaccat tccagaaaag tttttaatta ttttttcaaa tttttttaaa
     1021 acttctattt gtcttaattt aactatattt ctataaatgc tggaaatttt gttttttttt
     1081 aagtattcat ctgctttttt attaagtaaa gtttcaaata tatctttgta ctctttaatt
     1141 ttttcaacat ttatgtattt ttctttctca aaagtttctt taatactttc atgaattatt
     1201 tttccccaca tatctggaat ttctcttatt tcaggaattc tatatggttt aatttcttcc
     1261 accttctgca agtaaaattt ataaggacat ttaagtaata attccaaatc tgaaactcca
     1321 atatttaact ttccattttg gatgtatttt ttcttaaatt tgcttatatc tattttaata
     1381 tcaaatattt tttttgaatc aatagaaaat tcttttgtta tttcttcttt tgggctgata
     1441 tcaaaaataa aaattgattt catttgaagt tgattttcaa cccttgatgg aaaagtgaaa
     1501 attacttttt catgttcatt tctgattctg taaaaatcac atctatctct tgctattctt
     1561 ttttcaaaat attcaagtcc aagttctttt tttactttat caggtagtaa aaactcttca
     1621 attttaggtg cattgggaaa attttcttct gttgcaccac agaaaaaaca aatttttttt
     1681 tcaataccca gactttccat tattccactg actctaattc ccataccttt tccttcttct
     1741 atttctatca tttccagtaa tttttttaat aaatttatga attcttcctt tttcaaaaga
     1801 tatgaaccat tcattttatc aattaatttt tcaaactcaa tttttatttc aggttcagaa
     1861 gtatgccatc cggtcatttc tataattttt tctaaagtat ctttccattc agagatagtc
     1921 atttcttttt ttattaaatt tgtacactct tttaatattt caatactttt ataatttatt
     1981 ttttcaaaat caaaattttc tttataaaaa cctatatttt catattcttc tcttattttt
     2041 tctgaaaagt tttcaacttc attttggttt atttttgaaa aataagggga ggttagaata
     2101 ttcattaaag tattccattc gtatgtttca gcgagagtga aaaattcaag aagaggagaa
     2161 attgaaggtt caataataaa ggaatacccg ggaattaatt caacaggtaa attgaatctg
     2221 gaaaatatcc tttttatata atttctataa tttaacatat caggacaggt tataagaaaa
     2281 tcgtcaagac ctatttcttt attattttta aattctctct ctattaagtt gataatacct
     2341 ttaatttctt cttcttttgt gggataatta aaacattcaa tttctggttc atatttttca
     2401 ccatcaattt tttctatttc ccagtttatt atttttttta gaaaattaaa agtatcattt
     2461 attattaatt tctgtgtatc aatggaaaaa cagtcaggtt ggtaataaga aaaaatcacg
     2521 gattcggttg acttcacaag tttagcaata aaatttcttt gataattcgg aatttctact
     2581 atattttcaa atataatact ttcataattt tcagtaagat gatttgttgc ctcatcataa
     2641 atatcttcaa tatctataag atttcttttg tataaataat tttcgtattc ttcaaataaa
     2701 tttaaagcca attctatatt tttcttatga ttttcaaact tgaaaggaaa accagagatt
     2761 atgtttttta cttctgataa ttttaccttt tgtattgaag aaattttaag ttcttttata
     2821 aaatttaaaa ctatctttgc aaatccctca tctgtatatc cataaaattt ttcttttttt
     2881 tcctttttga taatgtcaat aataattaga aatctttcta catcagaaat aatttttttg
     2941 ctactttttt ctgatatgat ttttgaggcg aatgatttta atgtaaaaga aaaagggaga
     3001 gcagaatttt taaagtaatt gtaaaaatta agtttgaaat cttgaacctt acagaaattt
     3061 gatgtaatat aaataaaatt atttgctaaa tttagtttat caatttgaga taataaaaat
     3121 ttagttttat ttttaagatt taaaggtaac agaaaaattt ttttcttcat ttttatatat
     3181 tatacttaaa gattataaaa aaggtcaaag gataaattgg gagttttaat gaaatttaaa
     3241 aaaattgtga gaaatttttt aaagccattt gaaaatggaa ttataattta cactttttta
     3301 agggttctca ctatctttat tcaagttata cctgtttttt tatgtgaaaa aataataatt
     3361 tttctcggga attttgcatt ttttgttatg aaaggacaca gaaagttggc tttaaaaaat
     3421 atggatattg cttttggaga taaatataca aaaaaagaaa aaataaaaat gtgtaaacgg
     3481 ctatttatag agtgtatgag ttttttagaa atatttcaac tgtataaatt taaagatgaa
     3541 gaacttatga aaatggctga aatagaagga gaagaaattt taaagcaggt ggtaaaagaa
     3601 aaaaaaggag ttattgcagt atgtccacat atgggaaatt tcccatttgc tcttgttatt
     3661 cttactaaaa aaggatatcc ggttaatatg attgtagcaa ggtcagtaaa tgtgtataca
     3721 gatagagatt tgagaaaact gagagaaaaa tttggtgtcc cctcaatttc aagaaaagac
     3781 ctgaaaaaag cgattgaaga ttctcagaga tggattaaaa atggtggaat tttatgtata
     3841 tatcttgacc agcatgctcc aaacggaata aaatgcgaat tttttgggaa agaagtttat
     3901 gttcctgcag gtgctgctgt ttttgcaaga aaatataaaa caaatgtaat tggattatat
     3961 acagtgagaa caggtccaat gaaacacaag atagtaattg aaggtcctta taaacttaaa
     4021 gaatccagaa atgtgtctga agatgtaaag gaaaataccg cctttttcat taaaagggtt
     4081 cagaactggg ttgaaaagta tccggaacag tggtcatcct ggttaaatcc cggtagattc
     4141 cctgattaaa aaaaaatctt ttaaattttc aactatttcc tttttcaaat ttattctttc
     4201 tggattattg attatttttt ctattttttt taaatttttt agagcataat cttgaccaat
     4261 ttttatacat tcttttaatt ttgagaaatt aaaccattct acagaagata attctatttt
     4321 taaaacaaaa tcagagagag aactttctaa aatgtttgaa taatggtttt ttattttttc
     4381 tatctgaaca aatacttcaa aagcattcaa tggtttatgg tttttcagta gagagttatt
     4441 ttctatccca ataattactt cgcctccaag tatatgattt gcaataaccg gaagattact
     4501 tgttacccct ccatcaacat aataattatc acttattttg acaggtgtta caagtccagg
     4561 aattgcacag gaagcgatta gagatggaat gatttttcca ctattaaata taacgggtct
     4621 ccctgtaaaa tattcagtgg caattatacc aagtgtattt ttctgctctt caaaataaat
     4681 ttcttttcca acatatttat aaattttttc cttaattatt tcagactcaa taagtcctga
     4741 tttaaaacta tcaaaaagga gtaaagaaaa atcttttatt ccatcaataa atctgtaaag
     4801 ataacttctt tttctttttc ttgaaaaaag tttttctatt ttttgtaatt cccttattga
     4861 aaatatttta aaaataattt cttcaagttt ctctgcactt tttgtaaacg aataaattgt
     4921 tcctattaaa gctcccatac tagttcctgt aataacttca aaatcaaaac cttctttttc
     4981 aaaaacttta agaacaccga tttgacaaaa actctttgcc cctccaccac aaagagcaat
     5041 tccttttttc atattaagtt atttaatata ttcataaaaa aagcagttat aattcctact
     5101 gttagattat catctatttt aagaggcaaa atttcaacaa ttgaagatat taaaactcct
     5161 gataataaaa tataaaatgg taaattcaaa aaattatgaa gaaaaaaagc aattatcaaa
     5221 ttaaaaataa ttcctcctat gaacccctca agagttttac ctggaaaaat ttttgtttta
     5281 ccatatttac ttccaagaat tccacttcct gcatcaccaa aaataaggaa aaataaagaa
     5341 gatattgcaa tatctttttt gaaaaaaagt aaaattatga aagtggcaag cataaaatag
     5401 gtatcaccag ttaattttcc agggggtgtt ttaaaaattc taccatactt tcttaaaaca
     5461 taatcccata cacctggatt tttccatctt tcaaattcaa gagaaaaaag tagggttaaa
     5521 aaaaaagaac atattatcag agagaagaat aacgaatttg tgaataaata aactgaagga
     5581 ataattgaac ccataaaaat tctatacatt tttcttttcc agattaccat ttttaatcct
     5641 ttttgtattt tttatttcct gaattacatc tttattgata atagcaccaa ctaaaataag
     5701 gatcagggta gaaataaaaa gttgaaaaaa gtaagttttg taaaaaataa aaaggaatat
     5761 aaaaaatatc agaatacaag aagttaaaat tttaggagaa cgaggattta ttttctgagc
     5821 aaaaaaccat aaagcaatta caagaggaaa aagtttaggc attaaataca aaataatacc
     5881 accacttgta gcagctcctt ttccaccttt aaattttaag aaaattgaat aacagtgtcc
     5941 tattacagca gaagaaccaa ttaaagtaag aagtataaaa tttgtaattc catatttgtt
     6001 cgcaagaaac attggaaata caccttttat tgtatcagcc aaccagacaa taatgcccca
     6061 ttttttccct aaggttcttg caacattagc tgccccggga tttcctgttc caatagttct
     6121 tatatcctgt cctgtgagga aataagtgaa tagatatgca aaagaaatac tacctacaaa
     6181 ataactgaaa ataacaaaaa ttatcacttt catcaattgt attttaatat tttcttgaag
     6241 gattagtcaa ttatgttttt gaattttatt tacctaatgt ttttattaat ttagttaaat
     6301 ggaaatttta atcttacagt ttaacaggtt gattttttaa tatactatca tgtgctttta
     6361 aagttatttt taatgttttt aatgcatctt tataatctga ttttataagt tcaggatttt
     6421 tctcccaaac gctttttata aatgcaaagt cctcttgata catcgggtca acatttgata
     6481 tatacttttc gcttctatct cccttattta tttcaatagt attaccctta taaaagaaaa
     6541 tcacatcttt tccaataatt tcaatatcac tttcaaaacc tttccataac catgtgcatg
     6601 taagatttcc gataattcca tttttaaatt tcaaaatcat tgttaaacca tcttcaacat
     6661 catattcttt aaaaatttta ttattaattc cttcaaattt gtatgcataa atttcttcta
     6721 catctccaca gaaatatctc atcaaatcaa ccgtatgagt tgcctgttca attaactgac
     6781 cgcctgacat atcttttttt attaaccatc tttttcttcc tgctccaggg acctctccgt
     6841 aatatctacc cctgacaatc ccaatttttt ctgtttcagt caattttttc attttttcaa
     6901 taatatcaaa atatctcagt acatatccta cagatgttat taatttttta ttttctattt
     6961 ttttagcaat ttcctctgct ttttccaaat tcaaatgtat gggtttttct acaaaaaaag
     7021 gtatacttct ttcaatacat tcttcttcct gacctatatg ggcaaaagga ggaacacata
//
LOCUS       DAOSAT010000093         6858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_7106_length_6858_cov_5.868440, whole genome shotgun sequence.
ACCESSION   DAOSAT010000093 DAOSAT010000000
VERSION     DAOSAT010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7106_length_6858_cov_5.868440"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..384
                     /locus_tag="PLF90_07710"
     CDS             112..384
                     /locus_tag="PLF90_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOK57199.1"
                     /translation="MKRYHLYVKGIVQGVGFRWYAQRIGNSIGVSGWVKNLPDGSVEI
                     VVEGEKEKIEIFIEEMKNGYLGKNITEIEKIEEDFKGEFKGFEIRF"
     gene            387..761
                     /gene="queF"
                     /locus_tag="PLF90_07715"
     CDS             387..761
                     /gene="queF"
                     /locus_tag="PLF90_07715"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOK57200.1"
                     /translation="MKNYKDVAKEGLDAELPEIECFENQFPSYTITIIIPEYTSLCPK
                     TGQPDFGTITIEYEPDKKVIELKSLKIYIQAYRNLGIFYENAVNRILRDIVKAVNPKW
                     VRVKGEFNPRGGIKSIIEAKYP"
     gene            772..1743
                     /locus_tag="PLF90_07720"
     CDS             772..1743
                     /locus_tag="PLF90_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOK57201.1"
                     /translation="MKKNELPRKVIIGTIIKSFWEEYPGIEKRLSQLSQIIDEINLTS
                     LKIYNRNVDIIVLPEAAVTGGRKGRALERSLPFKGKIQLTFSFLAKKYQCYITVPMEL
                     IEDEERKICYNACILIDRNGMTMGIYRKLHPAIIFGSEDMEGGITPGKEVPVFECDFG
                     KVGIQICFDMEFDYGWKEMAKKGVELVLWPTQSPQTSHPVFRAMQYRIYIVSSTWRNN
                     ASIFEPTGKITAQIKEPEKILVKEIDLSYVILPWAPQLKNGEALREKFGDKIGFRYYE
                     DEDCGIFWSNDFSLPVSKMVNEIGLGDWSLEKKRLIKFFNKIRTRRY"
     gene            1743..2933
                     /locus_tag="PLF90_07725"
     CDS             1743..2933
                     /locus_tag="PLF90_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57202.1"
                     /translation="MKIFKEFFLFSVLFIFAGQMKVISQSVINPMDEYLKKWEIYIIN
                     VSKSRYCDKEMGEEIGWLVSPFLNGFYYGYMATEDTKWIDMLIDWTDSLLKRAIKEPD
                     GYIGWPKKGSGGSYAGKFYTDSLLGEAMVFCPVVKMSKVILETPLLKEKYGKKAEEYI
                     KLAEKIFEKWDKRGCWRETKAGGVWVVPPFGIDETTGKWTEGYERKEKDGFTNPANKQ
                     NYIAQYLIALYDCTGKPVYKERAEKWFKEMKSRMKLREGKYYVWNYWDPAGEWDYNPD
                     GSPKHWIGVHPNGGYYSIDVEGIVTAYEHGIVFTKEDIDILIATNRDFMWNGEIKGAK
                     FKRIDGGKPDPRWPDTPGVLWTSLIFYDETLRKIFEANHKPDSWSGLKITPWYFTKLK
                     ELQK"
     gene            complement(2924..3943)
                     /locus_tag="PLF90_07730"
     CDS             complement(2924..3943)
                     /locus_tag="PLF90_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57203.1"
                     /translation="MGKTILKTEFFIEVFKDGFKILKSKQRILSIFILLFIFENFFSI
                     LISYFYHYKPLISTSFLKFFTEIEFRSLWITFKKEFISSTGQLPFSICSVSVPYNITV
                     ILFLVFSFKIWRKISDDFPEDLKTTKIFLLKNFFPFLIACLITLSPFYIKKLSQLIFN
                     KNLIVWSFITIPFALYSFTLLSAVLNSYIFYLLSSEEEKEIFDFHALKFTRSIFLIKL
                     ILLAPLYIFSIFLPYTKFTIYMAKFFHAILIFFFFVPPLIVIENLEWQQAFKRNFEFL
                     RDYRVLIIIYFFIIYCLNFLLKFIQLFFNSKLLDLIYVFINIPFEFYIVAVTCVFLKK
                     IGSYF"
     gene            complement(3943..4818)
                     /locus_tag="PLF90_07735"
     CDS             complement(3943..4818)
                     /locus_tag="PLF90_07735"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOK57204.1"
                     /translation="MGKVLVFGEVLFDLIEGNEYLGGAPFNLACHLKKIGMDVIFISA
                     VGKDERGEKILKAMEKFNLSSEFIKIIDDVPTGIVNVKLNKNQPEFEIVYPGAWDFIE
                     LEEEKIRKLQNKKIEMFCFGSLALRSDKTKNTFFRLNSVLKNCKKFCDINFRKPFYNK
                     ELVENLLKISDILKLNENELKETGEMFGIKGSYEEIMSKLSEKFNIEVICTTLGENGA
                     IMFWDYKFFKEEGIKVEVVDTVGAGDAFSAGFIKGYIEKLAPEEILKIANKLGAFVTS
                     KRGAIPDYEYKELIK"
     gene            complement(4819..5394)
                     /locus_tag="PLF90_07740"
     CDS             complement(4819..5394)
                     /locus_tag="PLF90_07740"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOK57205.1"
                     /translation="MFTGIIEETGKIKGKRGKGNGFEILIEGNKVFEDLKIGDSIAVN
                     GVCLTVERIEKNTFFVSISSQTKKQTNLGEVKVGEYVNLERALKIGDRIGGHFITGHI
                     DFKTRISLLRKEGEFYLMKIKIPEEYRKYFVKRGSIGVDGISLTIAEIIRDEILIFLI
                     PYTFENTNIKYREVGDSVNIEIDIFAKYLVS"
     gene            complement(5379..6509)
                     /gene="ribD"
                     /locus_tag="PLF90_07745"
     CDS             complement(5379..6509)
                     /gene="ribD"
                     /locus_tag="PLF90_07745"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOK57206.1"
                     /translation="MIEHKKFLKKCFQLAEKGKGLVSPNPLVGAVIAKGSRIIAEGYH
                     KGFGLLHAEIEALNKAKDKAKGVTLYVNLEPCCHWGKTPPCTDAIIKSGIKEVIACMI
                     DPNPLVNGNGFKILEENGIKVRYGFLEEEAREFNRFYITNITKKRPYVILKWAQTIDG
                     KIATYNGSSKWITGEKTREYLKKKRFEVDGILIGINTILKDNPSLDYIAPEFQTKKEI
                     IDRKRYFKIILDPEGKIPFDAKIWENEKSKVLIVFDEKIEDEKIEKFKIKTNFDYIKI
                     PFENGKFDINKLLEKLLIEKNIGILLVEGGKYTLTSFFENRIFDEIWAFIGNKILGGE
                     NSLSIIGGKDKENINEAVELEKIEIERIEDDILIKGRICLQE"
     gene            complement(6502..>6858)
                     /locus_tag="PLF90_07750"
     CDS             complement(6502..>6858)
                     /locus_tag="PLF90_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOK57207.1"
                     /translation="TDGFGADVVITACPAKKAQMDALEIIGSKGRISFFGGLPKDDSI
                     IQIDSNIIHYKEISVFGAFASNRNDYIKGADLVSTGKIDAKKFITDVIPLEKIVEGIK
                     NVRTGDALKIVVKIND"
BASE COUNT         2497 a         1025 c          988 g         2348 t
ORIGIN      
        1 tgattacaga aatacctgaa aagaaagaaa aaacagcaac cccacctcct taccctgaat
       61 attaatgaag gaagcccgcc acttaattgt ggcgggcttt tttattttaa aatgaaaagg
      121 tatcatctat atgtaaaagg tatagtccag ggagttggat tcagatggta tgcacagaga
      181 ataggaaatt ctattggagt aagtggatgg gtgaagaatt taccagatgg ttctgttgaa
      241 atagttgtgg aaggagaaaa agaaaaaata gagatattca tagaggaaat gaaaaacggg
      301 tatcttggaa aaaacataac agaaatagag aaaattgagg aggattttaa aggagagttt
      361 aaaggttttg aaataaggtt ctaaaaatga aaaattataa agatgtagcc aaagaaggac
      421 ttgatgcaga attacctgaa attgagtgtt ttgaaaatca attcccatcc tatacaatta
      481 caattataat tccagaatat acctctctat gtccgaaaac cggccaacct gattttggaa
      541 caataacaat tgaatatgaa cccgataaaa aagttattga attgaaatca ttaaaaatct
      601 atattcaggc atacagaaac cttggaattt tttatgaaaa tgctgtcaat agaattttaa
      661 gagatatagt aaaagctgta aatccaaaat gggtaagagt taaaggtgaa tttaatccaa
      721 gaggtggaat aaaatcaata attgaggcaa aatatccata acctataata tatgaaaaaa
      781 aatgaattgc ctcgtaaagt tattatagga acaattataa aaagtttctg ggaagaatat
      841 ccaggaattg aaaagaggtt gtcacaactt tcccaaatta tagatgaaat aaacctgact
      901 tctttaaaaa tatataatcg taatgttgat attattgtgc ttccagaagc agcagtaaca
      961 ggtgggagaa aaggcagagc acttgaaagg tctttacctt ttaaaggaaa aattcagcta
     1021 actttttctt ttttagcaaa aaaatatcag tgttatatta ctgtacctat ggaattaatt
     1081 gaagatgaag aaaggaaaat atgttataat gcatgtattc taatagatag aaatgggatg
     1141 acaatgggta tttacagaaa acttcatcct gcaataatat ttggaagtga agatatggaa
     1201 ggtggaatta ctcctggaaa agaagttcct gtttttgaat gcgattttgg aaaagttgga
     1261 attcaaatat gctttgatat ggaatttgat tacggatgga aggaaatggc aaaaaaggga
     1321 gtagaacttg ttctatggcc aacacaatct cctcaaacaa gtcatcctgt ttttagagcc
     1381 atgcagtaca gaatttatat tgtttcaagc acatggagaa ataatgcatc aatttttgaa
     1441 ccaacaggga aaataactgc tcagataaaa gaaccggaga aaatacttgt aaaagaaatt
     1501 gatttgagtt atgttattct accatgggct cctcaattaa aaaatggtga agcattaaga
     1561 gaaaaatttg gagataaaat cggatttaga tattatgaag atgaggattg tggcattttc
     1621 tggtctaatg atttttcttt acctgtaagt aaaatggtaa atgaaattgg attgggtgac
     1681 tggagtttag aaaaaaaacg tttaataaag ttttttaata aaataagaac taggaggtat
     1741 taatgaagat atttaaggaa tttttcttat tttcagtttt atttattttt gctgggcaga
     1801 tgaaagtaat ttctcaatca gtaattaatc caatggatga ataccttaaa aaatgggaaa
     1861 tatacataat taatgtaagc aaatcaagat attgtgataa agaaatggga gaggaaatag
     1921 gatggctggt aagtcctttt ttaaatggtt tttattacgg atatatggca acagaagata
     1981 caaaatggat tgatatgctt attgactgga ctgattctct gcttaaaaga gcgatcaaag
     2041 aacctgatgg atatatagga tggcctaaaa agggttcagg aggtagttat gctggaaaat
     2101 tttatactga tagtttatta ggtgaagcca tggttttctg tcctgtagtt aaaatgtcaa
     2161 aagtaatcct tgaaactcct ttgttgaaag aaaaatatgg gaaaaaagca gaagaatata
     2221 taaaacttgc agaaaaaatt tttgaaaagt gggataaaag aggttgctgg agagaaacaa
     2281 aagcaggtgg tgtatgggtt gttcctcctt tcggtataga tgaaacaaca gggaaatgga
     2341 cagagggata tgaaagaaaa gaaaaagatg gttttacaaa tccagcaaat aaacagaatt
     2401 acattgctca atatttgatt gcattatacg actgcacagg aaaacccgtt tataaagaaa
     2461 gagcagaaaa atggtttaaa gaaatgaaat caaggatgaa attaagagaa ggaaaatatt
     2521 atgtgtggaa ttactgggat cctgctggtg aatgggatta taatcctgat ggctcaccaa
     2581 aacactggat aggtgttcat ccgaatggtg gatattattc tattgatgtt gaaggtattg
     2641 taactgctta tgaacacgga atcgtattta caaaagaaga tattgatatt ttaattgcaa
     2701 caaaccgcga ttttatgtgg aatggtgaaa taaaaggagc aaaatttaaa agaattgatg
     2761 gtggtaagcc agacccgaga tggccggata cacctggagt tctatggaca tctttaattt
     2821 tctatgatga gacattaaga aaaatttttg aagcaaatca taaacctgat agttggagtg
     2881 gtttaaaaat aactccatgg tatttcacaa aattgaaaga acttcagaaa taacttccta
     2941 tttttttcaa aaaaacgcag gttactgcaa caatataaaa ttcaaatgga atatttataa
     3001 aaacataaat gaggtcaagc aattttgaat taaaaaataa ttgaatgaat tttagaagaa
     3061 aatttaggca gtaaatgata aaaaaataaa taattattaa aactctgtaa tccctcaaaa
     3121 actcaaagtt tctcttgaat gcctgttgcc actctaaatt ttcaattaca ataagaggtg
     3181 ggacaaaaaa gaaaaaaatt aaaatcgcat gaaaaaattt agccatatat atcgtaaatt
     3241 ttgtataagg taaaaaaatt gaaaagatat ataatggagc aagtaaaatc aacttaatta
     3301 aaaaaattga ccttgtaaat tttaatgcat gaaaatcaaa tatctccttt tcttcctctg
     3361 aactcaacag atagaaaatg taactgttaa gaactgctga taataaagta aatgaatata
     3421 aagcaaacgg tattgttata aaagaccaaa caattaaatt tttattaaaa attaactgtg
     3481 aaagtttttt aatgtaaaaa ggggataaag ttattaaaca tgcaatcaaa aaaggaaaaa
     3541 aattttttaa tagaaaaatc tttgttgttt ttaaatcttc aggaaaatca tcagaaattt
     3601 tacgccatat tttaaaactg aaaacaagga atagaataac tgtaatatta tatggaactg
     3661 aaacagaaca gatggaaaaa ggaagttggc cagtacttga aatgaattct ttcttaaaag
     3721 ttatccataa acttctaaac tcaatttcgg taaaaaattt taaaaaagag gtagaaatta
     3781 agggtttgta atggtaaaag taagaaatca aaatgctaaa aaaattttca aagataaaca
     3841 gaagaataaa aatactaaga attctttgtt tagattttaa tattttgaat ccatctttaa
     3901 aaacttcaat aaaaaattca gtttttaaga tagtttttcc cattatttta taagttcttt
     3961 gtattcataa tcaggaatgg cacccctttt tgatgtaaca aaagcaccga gtttattcgc
     4021 aattttaagt atttcttcag gcgcaagttt ctcaatatat ccctttataa aaccagcaga
     4081 aaaagcatct cctgctccaa cagtatcaac aacctctact ttaattcctt cctctttaaa
     4141 aaatttataa tcccaaaaca ttattgctcc attttctcca agtgttgtac atataacctc
     4201 aatattaaat ttttctgata gtttactcat tatttcctca taactccctt ttataccaaa
     4261 catttcgcct gtttctttca gttcattttc atttaatttc agaatatcac ttatttttaa
     4321 aagattttca accagttcct tattataaaa tggctttctg aaatttatat cgcagaattt
     4381 tttacaattt ttaagaactg aatttaacct aaaaaatgta ttctttgttt tatcacttct
     4441 taaagcaagc gaaccaaaac aaaacatttc tattttttta ttctgcaact ttcttatttt
     4501 ttcttcttcc agttctataa aatcccatgc acctggatat acaatttcaa attcaggttg
     4561 atttttattc aattttacat tcacaatccc tgttggtaca tcatctatta tctttataaa
     4621 ttcacttgaa agattaaatt tttccatcgc ttttaaaatt ttttctcctc tttcatcctt
     4681 accaacagca gaaataaaaa taacatccat accaattttt ttcagatgac atgcaagatt
     4741 aaaaggtgca ccgccaagat attcattacc ctcaattaaa tcaaaaagaa cttctccaaa
     4801 aacaagtact tttcccattt aacttacgag atattttgca aaaatatcaa tttctatatt
     4861 aacagaatct ccaacttccc tgtactttat attcgtattt tcaaaagtgt acggtattaa
     4921 aaaaattaaa atctcatctc ttataatttc tgcaatagtt aaagatattc catcaactcc
     4981 aattgaaccc cttttaacaa aatattttct gtattcttct ggaattttta ttttcattaa
     5041 ataaaattca ccctcttttc tcaataaaga aatccttgtt ttaaaatcaa tatgtcctgt
     5101 aataaaatgt ccacctattc tatctcctat tttcaatgcc ctttcaagat tcacatattc
     5161 accaactttt acttcaccaa gatttgtttg ttttttcgtt tgagaactta tagatacaaa
     5221 aaaagtattt ttttctattc tttcaactgt cagacatact ccatttacag caatactatc
     5281 tccaattttt aaatcttcaa aaaccttatt cccttcaatt aaaatttcaa atccattccc
     5341 tttacctctt ttacctttaa tttttccagt ttcttcaatt attcctgtaa acatattctc
     5401 ccctttatta aaatatcgtc ttcaatcctt tctatttcta tcttttcaag ttcaactgct
     5461 tcatttatat tttctttatc ttttccacca ataatagaca aagaattttc tccaccaagt
     5521 attttattcc ctataaatgc ccatatctca tcaaaaatcc tgttttcaaa aaaagaagtt
     5581 agtgtatatt ttccgccttc aaccaaaaga attccaatat ttttttcaat aagcaatttt
     5641 tcaagcagtt tatttatatc aaattttcca ttctcaaatg gtattttaat ataatcaaaa
     5701 tttgtcttaa ttttaaattt ttcaattttt tcatcttcaa ttttctcatc aaaaacaatc
     5761 aaaacttttg atttctcatt ttcccatatt tttgcatcaa aaggaatttt tccttcaggg
     5821 tcaagaataa ttttaaaata tcttttccta tctattatct ccttttttgt ttgaaattca
     5881 ggtgctatat aatctaaaga agggttgtct tttaaaatag tattaattcc tataagaatt
     5941 ccatcaactt caaacctttt tttctttaaa tattctcttg ttttttctcc tgttatccat
     6001 ttagaagaac cattatacgt tgctattttc ccatcaattg tttgagccca tttcaaaatt
     6061 acataaggtc ttttttttgt tatatttgtt atgtaaaatc tgttaaattc ccgtgcctct
     6121 tcttctaaaa atccatatct tacttttatt ccattctctt caagtatctt aaaaccattt
     6181 ccatttacaa gaggattcgg gtctatcata catgcaataa cttcttttat tcctgattta
     6241 ataattgcat ctgtacaggg tggagttttt ccccagtggc agcatggttc aagatttacg
     6301 tataaagtta ctccttttgc tttatccttt gctttattca atgcttcaat ctctgcatgt
     6361 aaaagtccaa aacctttatg atatccttcc gctattattc ttgaaccttt tgctattact
     6421 gcaccaacaa gaggatttgg actaacaaga cctttccctt tttcagcgag ttgaaaacat
     6481 tttttaagaa actttttatg ttcaatcatt tattttcacc acaattttaa gtgcatctcc
     6541 tgtccttaca ttttttattc cctcaactat tttttcaagc ggaattacat ctgttataaa
     6601 tttttttgca tctattttac ctgttgaaac taaatcagca ccttttatat agtcattcct
     6661 gtttgaagca aaggcaccga atacagatat ttctttatag tgaattatat ttgaatcaat
     6721 ctgaataatt gaatcatcct ttggtagtcc tccaaaaaaa cttatccttc cttttgaacc
     6781 tattatttca agagcatcca tctgtgcttt tttagcagga catgcagtaa taacaacatc
     6841 tgctccaaaa ccatctgt
//
LOCUS       DAOSAT010000094         6765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_7240_length_6765_cov_4.592697, whole genome shotgun sequence.
ACCESSION   DAOSAT010000094 DAOSAT010000000
VERSION     DAOSAT010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7240_length_6765_cov_4.592697"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PLF90_07755"
     CDS             <1..923
                     /locus_tag="PLF90_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK57208.1"
                     /translation="KKFIKEKVNELKKKIKDKKALVATSGGVDSCVCAILTKMAIGNN
                     LIVFFVDDGLMRYKEAEEVRDFFAKKGIDLEIWNVEDAFFDALKGKIDPEEKRKAFRD
                     TFYKILSQAVRSYSVDFLIQGTIAADIIETQKGIKTQHNVLSQIGVNPLHYGLTIVEP
                     LKELYKHQVRQVAKTLGLPKKIYDRKPFPGPGLATRIIGEVTRDRVEKIRIATKIVEE
                     ELTEKNIFQAFPVLFSDKATGIVEGKRLLGDIIAIRAVKSEDALTATPVKILWKRLFK
                     IRDRILKEVPGVVKVLYDITPKPPSTIEYI"
     gene            1002..1610
                     /gene="hisH"
                     /locus_tag="PLF90_07760"
     CDS             1002..1610
                     /gene="hisH"
                     /locus_tag="PLF90_07760"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOK57209.1"
                     /translation="MVGVIKYGAGNIYSVSSAIKFLGEKFVLVENPEIIDRVDFLILP
                     GVGSFDSGIENLEKTGILEKLKYRLELGIPFLGICLGLQLLFNVSEEGKKEGFGILKG
                     EVVKFKNEEIKIPHMGWNKVEILKDNKIFEGIVNNSFFYFAHSYYIKTQEDIIVGKTK
                     YGIDFPSVIIKDNIVGVQFHPEKSGKMGLLFIKNFLEGKWLQ"
     gene            1598..2356
                     /gene="hisF"
                     /locus_tag="PLF90_07765"
     CDS             1598..2356
                     /gene="hisF"
                     /locus_tag="PLF90_07765"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOK57210.1"
                     /translation="MVTVRIIPCLDVKDGKVVKGIHFEDIRVAGDPVENASFYSKEGA
                     DELVFLDISATIEGRKTMIDAVRKVADVVFIPFTVGGGISSLNDIEILLSNGADKISI
                     NTAAVKNPDLIKEGAKKFGSQCIVVAIDAKKTERETWEVYIESGKKPTGIDVLDWAKK
                     VEDFGAGEILLTSIDRDGTQDGYDIDLTRIVSENVKIPVIASGGAGKLEHLYEAVVKG
                     KASAVLIASLLHFRILTIPQIKKYLREKGVNVRI"
     gene            2382..3395
                     /locus_tag="PLF90_07770"
     CDS             2382..3395
                     /locus_tag="PLF90_07770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOK57211.1"
                     /translation="MKLRNIKDDGIKKILIIGFNSVGENLLITPAIKKIKDTYRNSQI
                     DIIVGDKAVEFVLDNPWFSSYFLWEKMGYYKLIKKLRNQKYDLIIDFKTPFFSFFLKS
                     KYKLTFFMKDILSDKFYIHQSEKIMKFLEPLVGPGEVKIFFPVSNYYREKVKEILISF
                     KIKSSDLVVVLIPGGEAVFKRWDKEKYVILGKELIKRYGVKIIIIGLKGEEKLTKEIK
                     DLIGSDNVFDLGGKTDLKENFVIFELSDLIVTNDSYLMYLACASGTDIVTIFGPGNPY
                     RYGPVGEKSYVVHSDIDCFPCSSYKKCKMEYQCIKEVKVEDVFKYCSIILDEKSYPKL
                     FEI"
     gene            3475..4464
                     /locus_tag="PLF90_07775"
     CDS             3475..4464
                     /locus_tag="PLF90_07775"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOK57212.1"
                     /translation="MEKFLITGGCGFIGSWLAEELIRQNKKVIVIDDLSTGSIKNVLN
                     IINHPNFSLFIDSILNRDIIEELIKKVDFVFHLAAIVGVKKVIDYPIQSIIVNIEGTH
                     NVLKFCAKYKKKILITSTSEIYGKNTNVPFNEDADIVIGSTKKKRWSYACTKAIDEFL
                     SFAFYEEYNLPVIIVRLFNTVGPRQTERYGMVIPRFVKQSLNGKDITVFGDGEQSRCF
                     IHVKDVVNCMVKLIEKEESYGDVFNIGSDKEIKIRDLAYLIKKITGSKSKIKFINPEE
                     VYSKGFEDMERRIPDLSKIKKILQFQPQYSIEDIINDVIEYYKKESFLSCYFF"
     gene            4525..6048
                     /locus_tag="PLF90_07780"
     CDS             4525..6048
                     /locus_tag="PLF90_07780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOK57213.1"
                     /translation="MKNKKLIIYSFLFFAFVIRILYLFFLKDTVFFDPYIMDKHDQKT
                     FIFWAEEILKHPFYVDGKVFYMAPFYPYLLSFLYLITGGNLFGIYIFQILIDVFLCYL
                     IYLLGKILVDEKTGIVACFFCAFYKTFVVYSASILSDSIITFLYIAFITSLYISLNKH
                     TLKWWILTGILLGFSALAKPTIGIFLPFLLIGLIIYPEKKIISLKISPPKQKILTFFI
                     LLFISGLVILPITIRNWYVGKVFVPICSNGPENWRIGNSSDSIGLFYYPKGHLLSVFS
                     ADFWKLFLRKLNLFFVSYEWPQNMNVYLMEKLIPSLKFTFIRFGFIVPSGLFGMILFF
                     KKFKKNFLYITFTISNVLWVVLFFITDRYRLPAVYCFSISASYLILWVLNEIFINKKL
                     LKSFILLLFVSIFAYMFNYAPSELLPEESKKIFGYLSAKGIEKDLREKKINNAFKKAG
                     DYLRILPDDPKSNFLMAIVYYESGDIKKTEYYLERALEIDPDFELAKNFLNDIMNKK"
     gene            6045..6446
                     /locus_tag="PLF90_07785"
     CDS             6045..6446
                     /locus_tag="PLF90_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57214.1"
                     /translation="MKKISFSIQNTLKSSISVIEELVTEIEEVLKDDKMIFTKIERKI
                     TGEKNKELKKKIKEIKIILQEIKRDLGLKEEVIIDKAVINSRCAKIWEILNDMKGEKL
                     KKYGNVSEDVKNYINSAVEKILKIVNEVVDL"
     gene            6460..>6765
                     /locus_tag="PLF90_07790"
     CDS             6460..>6765
                     /locus_tag="PLF90_07790"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOK57215.1"
                     /translation="MEIKFLNEEEIYKKCKEELEKREFKDKKVIVIIPDNTRSGPTWF
                     FFKTIIDILLQKVKKLDFMIALGTHPLMAEEKIKNFLNLKGEEKYVNIYQHQWDKPEN
                     "
BASE COUNT         2589 a          649 c         1194 g         2333 t
ORIGIN      
        1 caaaaaagtt tataaaagag aaggtcaatg aattaaagaa aaagataaag gataaaaaag
       61 cattggttgc gacttcaggt ggtgttgata gttgtgtttg tgcaatttta acaaagatgg
      121 ctatcggaaa caatcttatt gttttttttg ttgatgatgg attaatgaga tataaggaag
      181 cagaggaagt tagagatttt tttgcaaaga aggggataga tttagaaata tggaatgttg
      241 aagatgcatt ttttgatgca ttaaaaggaa agatagaccc tgaagaaaaa agaaaagcat
      301 tcagagatac attctataag atactcagtc aggcagtaag aagttattct gttgattttt
      361 taatacaggg aacaatagcg gctgatatca tagaaactca aaagggaata aagacacagc
      421 acaatgtttt atctcagatt ggagtgaatc cgcttcatta tggtttaaca attgttgaac
      481 cattaaagga attatacaag catcaagtaa gacaggttgc aaaaactctt gggttaccca
      541 aaaagatata tgatagaaaa ccttttcctg gtcccggtct tgctacaaga attattggag
      601 aagtaacaag agatagagtg gaaaaaatta gaattgctac aaaaatagta gaggaagaac
      661 taacagaaaa aaatattttt caggcatttc ctgttttatt ttcagataaa gctacaggta
      721 tagtagaggg gaagagattg ttgggagata taatagcaat aagggcggta aaatcagaag
      781 atgcattaac tgcaactcct gtaaaaattt tatggaaaag gttgtttaaa ataagagata
      841 gaatcttaaa agaagttccg ggagttgtaa aggtccttta cgatataaca cctaaacctc
      901 cgagtacaat tgaatatatt taattaatta aatttttctt tcttgctttt agagtatttt
      961 gaaattaaca ttttatagaa ttaaaattaa atcttattaa aatggtggga gttataaagt
     1021 atggtgcagg aaatatttac agtgtttcat ctgctattaa atttttaggt gaaaaatttg
     1081 ttttggttga aaatccagaa attattgata gagtggactt tttgattctg ccaggtgtcg
     1141 gaagttttga ttcagggatt gaaaatttag agaaaacagg aatattggaa aaattaaaat
     1201 accgattaga attgggtatt ccatttcttg ggatttgttt aggactacaa cttttattta
     1261 atgtgagtga agaaggaaaa aaggaaggtt ttggaatttt aaagggagaa gtggtaaaat
     1321 ttaaaaatga agaaataaaa attcctcata tgggatggaa taaagtagaa attttgaagg
     1381 ataataagat ttttgaggga atagtaaata attctttttt ttattttgct cactcttatt
     1441 atattaaaac tcaggaagat attattgttg gtaaaacaaa gtatggcatt gattttcctt
     1501 ctgttataat taaagacaat attgtaggtg ttcaattcca tcctgaaaaa agtgggaaaa
     1561 tgggactttt atttataaaa aattttctgg agggaaaatg gttacagtaa gaattatacc
     1621 ttgtcttgat gtaaaggatg gtaaggttgt aaaaggaatt cattttgagg atatcagagt
     1681 tgcaggggat ccggtagaaa atgcttcatt ttatagtaaa gaaggtgcag atgaactcgt
     1741 atttcttgat ataagtgcca caatagaagg aaggaaaact atgatagatg cggtaagaaa
     1801 ggttgcagat gttgttttta ttccctttac tgtgggagga ggaatttctt ctttaaatga
     1861 tatagaaatt ttattatcaa atggagcaga caaaatttca ataaatactg ctgctgtaaa
     1921 aaatccagat ttaataaaag aaggtgcaaa aaaatttgga agtcagtgta tagttgtcgc
     1981 tatagatgct aaaaaaacag aaagagaaac ctgggaagtg tatatagaga gcggtaaaaa
     2041 accaacaggt atagatgttt tagattgggc gaaaaaggtt gaggattttg gagcaggaga
     2101 aatattatta acaagtatag atagagatgg tacacaggat ggatatgata ttgatttaac
     2161 aagaattgta agtgaaaatg tgaaaattcc agttattgca tctggagggg ctgggaaact
     2221 ggaacattta tatgaagcag ttgttaaagg taaagccagt gctgttctta ttgcctctct
     2281 tttacatttc aggattttaa caattcccca gataaaaaaa tatcttagag aaaaaggagt
     2341 aaatgtaaga atatgaaata atttaaatta tttatcaagt gatgaaattg cgaaatatta
     2401 aagatgatgg tattaaaaaa attcttataa ttggttttaa ctcagttgga gaaaatcttc
     2461 ttatcacacc tgcgataaaa aaaataaaag atacatatag aaattcacaa atagatataa
     2521 ttgtaggaga taaagcagtt gaatttgttc tggataatcc atggttttca tcatattttc
     2581 tctgggaaaa aatgggatat tacaaactga ttaaaaaatt gagaaatcag aaatatgatt
     2641 taataataga tttcaaaaca cccttttttt cgttttttct aaaaagtaaa tataaattaa
     2701 ccttttttat gaaggatatt ttaagtgata aattttacat tcaccaaagt gaaaaaatta
     2761 tgaaattttt agaaccattg gttgggccag gggaagtaaa aatattcttt ccagtcagta
     2821 attattacag agaaaaagtt aaagagattc ttatctcttt taaaattaaa tcttctgact
     2881 tagtagtagt cttaattccc ggaggagaag cagtatttaa aagatgggat aaggaaaaat
     2941 atgtaattct cgggaaagaa ttaattaaaa gatatggggt aaaaataatt ataattggtt
     3001 taaaagggga agagaaatta acaaaagaga ttaaagattt aataggaagt gataatgtgt
     3061 ttgacctagg agggaaaaca gatttaaaag aaaattttgt aatttttgaa ctttcagatt
     3121 tgattgttac aaatgattct tatttgatgt atcttgcctg tgcttcagga acagacattg
     3181 taactatttt tggtccaggt aatccttata gatacggacc tgttggagaa aaaagttatg
     3241 ttgttcacag tgatatagat tgttttccgt gtagcagtta taaaaaatgc aaaatggaat
     3301 atcaatgtat aaaagaagta aaagttgagg atgtttttaa atattgttct attattcttg
     3361 atgaaaaatc ttatcctaaa ttatttgaaa tataagaatt tttgtagtta ttcaaaatag
     3421 aatataaaaa aaatctgtgg taaaatactc ttaataaact acttgaattt taaaatggaa
     3481 aaatttttaa taacaggtgg atgtggattt atcgggtcct ggttggcaga ggaacttata
     3541 agacagaata aaaaagttat tgttattgat gatctttcta caggaagtat aaaaaatgtc
     3601 ttaaatataa taaatcaccc taatttttca ctttttattg attcaatttt gaaccgggat
     3661 attattgaag aactgattaa aaaagttgat tttgtttttc atcttgcagc tattgttggg
     3721 gttaaaaaag ttatagatta tccaatccaa tcaataattg taaatataga aggtacccac
     3781 aatgtattaa agttttgtgc aaaatataaa aagaaaattt taataacttc aacttctgag
     3841 atttacggta aaaatacaaa tgttcctttt aatgaagatg cagatattgt tattggttca
     3901 acaaagaaaa aaaggtggtc ttatgcctgc accaaagcaa ttgatgaatt tttatctttt
     3961 gctttttatg aagaatataa tcttcctgtt attattgtga gattattcaa tactgttgga
     4021 ccaagacaga cagaaagata tggtatggtt attccaagat ttgtaaaaca atctctaaat
     4081 ggaaaagata taactgtttt tggtgatggt gaacagtcaa gatgttttat tcatgttaag
     4141 gatgttgtaa attgtatggt taaattgata gaaaaagaag aaagttatgg agatgttttt
     4201 aatattggaa gtgataaaga aataaaaata agagatttag catatcttat aaaaaaaatt
     4261 acaggaagta aatctaaaat aaaatttata aatcctgagg aggtttatag taaaggtttt
     4321 gaggatatgg aaagaagaat tcctgatttg agtaaaatta aaaaaatttt acaatttcaa
     4381 cctcaatatt caatagaaga tataattaat gatgtaattg aatattataa aaaagagtcg
     4441 tttttatcgt gctatttttt ttaatcaatc aattatcagg acgaaataga attataaata
     4501 gtataacgac tttattgata taggatgaaa aataaaaaac ttataattta ttccttttta
     4561 ttttttgcat ttgtaattag aattttgtat ttattctttc tgaaagatac agtttttttt
     4621 gacccatata taatggataa acatgaccag aagacattta ttttttgggc agaagaaatt
     4681 ttaaaacatc ccttttatgt tgacggaaaa gttttttata tggctccatt ttatccatat
     4741 ttactttcat ttttatattt gattacagga ggaaatttat ttggaatata tatatttcaa
     4801 attttaattg atgttttttt gtgttatttg atatatcttc ttggtaaaat acttgttgat
     4861 gaaaagacag gtatagttgc ctgttttttt tgtgcttttt ataaaacatt tgtcgtttat
     4921 tctgcttcta ttttaagtga tagtataata acttttctct atattgcttt tattaccagt
     4981 ttatatatat cattgaataa acatacttta aaatggtgga ttttaacagg aattttgctt
     5041 ggattttctg cacttgcaaa accaactatt ggaatttttt tgccgttttt acttattgga
     5101 ttaattatat atccagaaaa aaaaattatc tccttaaaaa tttcacctcc aaagcaaaaa
     5161 atattaacat tttttatatt actgtttata agtgggcttg taattctacc tataacaatt
     5221 agaaactggt atgttggaaa agtttttgtt ccgatatgtt ctaatggccc tgaaaactgg
     5281 aggattggaa atagttctga ttcaataggc ctcttttatt atccaaaagg acatcttctt
     5341 tcagtttttt ctgctgattt ctggaaatta tttttaagaa aattaaactt gttttttgtc
     5401 agttatgaat ggcctcagaa tatgaatgta tatttaatgg agaaactgat accatcatta
     5461 aaatttactt ttataagatt tggatttata gttccctctg gtctttttgg tatgattctt
     5521 ttttttaaaa aatttaaaaa aaattttctc tatataactt ttacaatttc aaatgtttta
     5581 tgggttgttt tattttttat tacagataga tatagattac ctgctgttta ttgtttttca
     5641 atatctgcat catatttaat tttatgggtt ttaaacgaaa ttttcataaa taaaaaactt
     5701 ctgaaatctt ttatcttatt actttttgtt tcaatttttg catatatgtt taattatgct
     5761 ccttcagaac ttttgccaga agaaagtaaa aaaatttttg gatatttaag tgcaaaaggt
     5821 atcgaaaaag atttaagaga aaaaaaaatt aacaatgctt ttaaaaaggc aggggattat
     5881 ttaagaattt taccggatga cccaaaaagt aattttttaa tggctattgt ttattatgaa
     5941 tctggcgata ttaagaaaac agaatattat ctggaaagag cacttgaaat agaccctgac
     6001 tttgaattag caaaaaattt tttaaatgat ataatgaata agaaatgaag aaaatatctt
     6061 tttcaataca gaataccctt aaatcttcaa tatcagttat agaagaactg gttactgaaa
     6121 ttgaagaagt tttgaaagat gataaaatga tttttactaa aatagaaaga aaaattacag
     6181 gagagaaaaa caaagagtta aagaaaaaaa taaaagaaat taagataatt ttgcaggaaa
     6241 taaaaagaga tttaggtttg aaagaagaag taattataga taaagcagtt ataaattcaa
     6301 gatgcgctaa aatctgggaa attttgaatg atatgaaagg tgaaaaatta aagaaatatg
     6361 ggaatgtttc tgaagatgta aaaaattata taaattcagc agtggaaaaa atattgaaaa
     6421 ttgtaaatga agttgtggat ttatgatgta aaatgaaaaa tggaaataaa atttttaaat
     6481 gaagaagaaa tttataaaaa gtgtaaagaa gaattagaaa aaagagagtt taaagataaa
     6541 aaagtaatag taattattcc tgataataca cgttcaggtc ccacttggtt tttctttaaa
     6601 acaataattg atattttact tcaaaaagta aaaaaacttg attttatgat tgctcttggg
     6661 acacatcctt taatggcaga agaaaaaata aaaaattttc ttaatttaaa gggtgaagaa
     6721 aaatatgtaa atatatatca gcatcagtgg gataaacctg aaaac
//
LOCUS       DAOSAT010000095         6690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_7346_length_6690_cov_6.365486, whole genome shotgun sequence.
ACCESSION   DAOSAT010000095 DAOSAT010000000
VERSION     DAOSAT010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7346_length_6690_cov_6.365486"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1713)
                     /gene="glmS"
                     /locus_tag="PLF90_07795"
     CDS             complement(<1..1713)
                     /gene="glmS"
                     /locus_tag="PLF90_07795"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOK57216.1"
                     /translation="MCGIFGFKGKGDVKEIIKTGLKILEYRGYDSCGFFFNSKEFAFY
                     NKKVGKDKIEELVESLPFKIDKEVIIISHTRWATHGLISEKNAHPHFDCKNEIAVVHN
                     GIIENYQFLKKQLEKNGHKFISQTDTEIVPHLIEENLKKDKNIISAVISSVKKMEGSF
                     ALLITKKGEDFIIGVKKNSPLVIGFDGENFYFASDPIPISQYTKKMIFLEENEIFYLD
                     SEIKFYNFKRDIEIEKRFEEIELKEIRISKENFDSYMLKEIYEQPESIERTLKYFLNE
                     TFEKLTKQFNPERIIITGCGTSWHAGLIGEYFIEDLLEIPVEVEYASELRYRNPVVDG
                     KTLLLTISQSGETADTIGAIKRLKSKCKIFSICNVEKSTVSRESDFVLMTKAGPEIGV
                     ASTKAFTSQILVLYLFVLTFLFKNRKIGKEKFFSKCNEILKLKERMVFFLKNNNVENI
                     AEKLIFKTNALYLGRRVNFPVALEGALKLKEVSYIHAEGYPAAEMKHGPIALIDENMP
                     VIFICVKDKLLKKVISNMEEVKTRKGYVISVCDFISDDIKKLSDEIILIPHIKNEYLK
                     VFLTI"
     gene            complement(1713..1841)
                     /locus_tag="PLF90_07800"
     CDS             complement(1713..1841)
                     /locus_tag="PLF90_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57217.1"
                     /translation="MIICDLCGKEKREEEIYAFYPEDSSYICKECFEKIKNKEKEQ"
     gene            complement(1857..>2093)
                     /locus_tag="PLF90_07805"
     CDS             complement(1857..>2093)
                     /locus_tag="PLF90_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57218.1"
                     /translation="YTVQQVEHLMKKAQKIIIVADDTKIGRDCIYQTRSMRMIKMDLK
                     NKKEYVLITTGSSEKNFISTIKKFKEIGIKVVLV"
     assembly_gap    2094..2193
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2194..2644)
                     /locus_tag="PLF90_07810"
     CDS             complement(<2194..2644)
                     /locus_tag="PLF90_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57219.1"
                     /translation="MGINDLFLSFEKRRDIEKEKKYKIAQKAIEFINIGEEIFLGAGT
                     TVSYVGELLAKSSSHFMLKVWTNNIFILNLYLKEYDKFFSENFVGIVSGEISRKNLSI
                     VNIYLPFSKVDKLIIGTPGFSVKGLTSDDAYTVQQVEHLMKKAQKIII"
     gene            2818..3111
                     /locus_tag="PLF90_07815"
                     /pseudo
     CDS             2818..3111
                     /locus_tag="PLF90_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
     gene            3219..4421
                     /locus_tag="PLF90_07820"
     CDS             3219..4421
                     /locus_tag="PLF90_07820"
                     /inference="COORDINATES: protein motif:HMM:NF039390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57220.1"
                     /translation="MMLREGWDVQNVSIVLGLRPFTSQAQILPEQAVGRGLRLMSGIS
                     PDHTQTLEVMGTEAFEEFVRELEKEGVGINTTKTPPPLPVTIAPEKSRLIYDIEIPQT
                     EFRFSRNYKKIETLDPLKIPSLYSSDKLEEERKIFLKMEFPVTETEVHIASLPATTLS
                     SGRELVSYIVREVMKRTRLTCEFNNLYPIVERYLLERCFETKIKDIEDDKLRRHLTDI
                     SIQDAIIDLLVKKIGEISIETKKEVVKLGSFKLSKIPPFIWRRKHLRCKKTVFNFVAV
                     YNDFEAEFAEFLDKCSDIEKFAALAEKFSIDYLSSRGAIRFYYPDFVAIQKSNGEIIH
                     WIIETKGREYEDTDKKDEAIKKWCEDISKQSKTEWKYLKIHQYNFEKLKNTCNTFSEL
                     LKNLFNTA"
     gene            complement(5014..5098)
                     /locus_tag="PLF90_07825"
     tRNA            complement(5014..5098)
                     /locus_tag="PLF90_07825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5063..5065),aa:Leu,seq:taa)
     gene            complement(6200..6418)
                     /locus_tag="PLF90_07830"
     CDS             complement(6200..6418)
                     /locus_tag="PLF90_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK57221.1"
                     /translation="MAEKILKRLLNIKEANQYLGISSKTLYKWVNEKRIPYVKVGGKF
                     LRFDIEKLNKWIEQHTVEELKILKNLEI"
BASE COUNT         2186 a         1083 c          829 g         2492 t
ORIGIN      
        1 aattgttaaa aatactttta aatactcatt ttttatatgt ggaataagta taatctcatc
       61 tgataatttt tttatatcat ctgaaataaa atcacaaaca gaaataacat aacccttcct
      121 tgttttcacc tcttccatat ttgaaattac ttttttcaaa agtttatcct ttacacatat
      181 aaatattacc ggcatatttt catctatgag tgctattggt ccatgtttca tttctgctgc
      241 tggataacct tctgcatgaa tataacttac ttctttcaac ttcagtgctc cttcaagtgc
      301 aacagggaaa ttaactctcc ttccaagata taaagcattt gttttaaaaa ttaatttctc
      361 tgcgatattc tcaacattgt tatttttaag aaaaaaaacc attctttcct tcaattttaa
      421 tatctcatta catttactaa aaaatttttc ttttcctatt tttctatttt taaaaagaaa
      481 ggtaagaaca aaaagatata aaacaagaat ctgtgatgta aatgcttttg tagaagcaac
      541 tccaatttca ggacctgctt ttgtcattaa aacaaaatca ctttctcttg aaacagtact
      601 tttttccaca ttacatatag aaaaaatttt acatttactc tttaatcttt ttatcgctcc
      661 aatagtatcc gctgtttctc ctgattgaga gatggtaaga agaagagttt ttccatcaac
      721 gactggattt ctgtatctta attctgatgc atattcaact tcaacaggaa tttcaagtaa
      781 atcttcaata aaatattctc caataagacc tgcatgccaa cttgttccac aacctgttat
      841 aataattctt tctggattaa actgttttgt taacttttca aaagtttcat tcaaaaaata
      901 tttcaatgtt ctctcaatag attcaggttg ctcatatatt tccttaagca tataagaatc
      961 aaagttttct tttgatattc ttatttcttt taactcaatc tcctcaaacc ttttttcaat
     1021 ctctatatcc cttttaaagt tataaaattt aatctctgaa tcaagataga aaatttcatt
     1081 ttcttcaaga aaaatcattt tttttgtata ttgggagatt ggaattgggt cagaagcaaa
     1141 ataaaaattt tctccatcaa aaccaataac aagtggagaa ttttttttta cacctataat
     1201 aaaatcttct cccttttttg ttatcaataa agcaaaagaa ccttccatct ttttaacaga
     1261 agaaataaca gcagaaatta tatttttatc tttttttaaa ttttcttcta ttaaatgggg
     1321 tacaatttct gtgtctgtct gggaaataaa tttatgccca tttttttcaa gttgtttttt
     1381 caaaaattga taattttcta taatcccatt atgaacaact gctatttcat ttttacagtc
     1441 aaaatgagga tgagcatttt tttcagatat taaaccatgt gttgcccatc ttgtatgcga
     1501 aattataata acttctttat ctattttaaa aggcagtgat tcaacaagtt cttctatttt
     1561 atctttcccg acttttttat tataaaaagc aaattcttta ctattgaaaa aaaaaccaca
     1621 ggaatcataa cccctgtatt caagtatttt taacccggtt tttattattt cctttacatc
     1681 tcctttgcct ttaaaaccaa aaataccaca cattactgtt ctttctcctt atttttaatc
     1741 ttttcaaaac attctttaca gatataactt gaatcttcgg gataaaaagc ataaatttct
     1801 tcttctctct tttctttacc gcataaatca cagattatca tttttctacc tcctttctaa
     1861 actaaaacaa cttttattcc aatctcttta aattttttta tggttgatat aaaatttttt
     1921 tctgatgaac ctgtggttat tagtacatat tctttcttat tttttaaatc catttttatc
     1981 attctcatac tccttgtttg atagatacaa tcccttccta tttttgtatc atccgcaact
     2041 attataattt tctgtgcttt tttcataaga tgttccacct gctgaactgt atannnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctattat aattttctgt gcttttttca
     2221 taagatgttc cacctgctga actgtatagg catcgtcaga tgttaaacct tttacagaga
     2281 agccgggagt tccaataata agtttatcaa ctttagaaaa tggtaagtat atattaacta
     2341 tgcttaaatt tttccttgat atttcgccac tgacaatacc aacaaaattt tctgaaaaga
     2401 atttatcata ttctttcagg taaagattta aaataaaaat attatttgtc catactttta
     2461 acataaaatg agatgatgat tttgcaagca gttctccaac ataagaaaca gttgtaccag
     2521 cacctaaaaa tatctcttct ccaatattaa taaattcaat tgccttttga gcaattttat
     2581 atttcttttc tttttctata tctcttctct tttcaaaact taaaaaaagg tcatttattc
     2641 ccatgcaaaa aattttacat aatgttttac aatttgtcaa gtaatttgtt aaattttttg
     2701 tcaaattaaa aaaatcttat ctaaccatta accaaattag caaaaatttt tttaaaagta
     2761 ttttcttttt ttctaaattt ttgtgataca ataacttctt aaaaagaagg aaagaatatg
     2821 ggaaaaaata ttatctatct tcattcaaaa tttaaaattg atagggttga cagaagaata
     2881 tttggtggtt ttcttgagca tattggaaga gcggtttatg gaggtgttta tgaacccaaa
     2941 agtcctttat ctgacgaaaa tggttttaga aaagatgtaa ttgaaaattt aaaaagatta
     3001 aaaatgacag ttatgagata tcctggtgga aattttgcat caggttatca ctggatggat
     3061 ggaattggac cgaaagaaaa aagacctgtt gtaagagaca ttgcctggca ggtaaatgaa
     3121 ccgagataaa aatctctgaa aaagaccttc caaaattaag agaattagcg agaagaatag
     3181 atgagcctga taacaaaatc aaaataatcg taagcaatat gatgttaaga gaaggctggg
     3241 atgtgcaaaa tgtaagtata gtattaggtt taagaccatt tacatcccaa gcacagattt
     3301 tacctgagca ggcagtggga agaggactta gattaatgag tggtataagc ccagaccaca
     3361 cccaaaccct tgaagtaatg ggaacagaag cgtttgaaga gtttgtccgt gaacttgaaa
     3421 aagaaggtgt gggtattaat acaacaaaga caccaccacc tctgcctgtg acgattgcac
     3481 ctgaaaaatc aagattgatt tatgatattg aaattcctca aacagagttt aggttctcaa
     3541 gaaactataa aaagatagaa actttagatc cattaaagat tccatcgctt tattcctcag
     3601 ataaacttga agaagagaga aaaatttttt taaaaatgga gttccctgta actgaaacag
     3661 aagtccacat cgcctcgtta cctgctacta ctctgtcttc tggtagggaa cttgtctctt
     3721 acatagttcg tgaggttatg aaaagaactc gccttacttg tgaatttaat aatttatacc
     3781 caatagttga aagatacctt cttgaaagat gttttgaaac aaagataaaa gatattgagg
     3841 acgataaatt aaggagacac ttaaccgata tttcaattca ggatgcaatt attgatttat
     3901 tggtaaaaaa gataggcgag atttctattg agacaaaaaa agaagtggta aaattgggtt
     3961 ctttcaaact ttctaaaatt ccaccattta tttggagaag aaaacattta agatgtaaaa
     4021 agaccgtatt taactttgtt gctgtttata atgactttga agcagaattt gcagaatttc
     4081 ttgataaatg ttcagacatt gaaaaatttg ctgcgctggc tgaaaaattt tcaattgatt
     4141 atttaagttc aagaggtgct ataagatttt actacccaga ttttgtcgct attcaaaaaa
     4201 gtaacggaga aataattcat tggattattg aaacaaaggg gagagaatat gaagatactg
     4261 acaaaaaaga tgaagcaatt aagaagtggt gtgaggacat tagtaagcaa tcaaaaacgg
     4321 aatggaaata tcttaaaatc catcaataca attttgaaaa actaaaaaac acttgtaaca
     4381 cttttagtga actcttaaaa aatctgttta atacggcgta agacaggaat taatttaata
     4441 ttgacaaatt ttttaggaag gaggtattat agggttatgt tcgtttttag ccactaaatt
     4501 tttaatcttg atctccgctg tccaccttaa aggtgggata gggaaatttt ctcaactttc
     4561 atcaagaatt acaactttac tttcggtcca gtatggtaat ttgtattcaa gtaaatctcc
     4621 ttcaagtttc agtattcctc caacttcttc ctgtggtatc attcctaaat gtactacaac
     4681 aggttcagca gtatattgta aggcaatttc ataagcaaga gcacttttac caactcctga
     4741 taccccaaca attaaaggat gcagaacagg aattttaagt ggattggctt ctttttctct
     4801 ttttttaact gatatgaaat aaaatcaagt attgatttaa ctttatctaa aatcttcata
     4861 aaaaataatt ttttttgttt ctattttcac aatatattat atcataaaat cggcaaaaag
     4921 tcaaattttt caacatattt ctctcaggtt aaaaaatcag gataggtaat ttcaaagtgt
     4981 gaccagttaa aattaaattc tataaataaa aaatgccggg agagggattc gaaccctcac
     5041 gggagcaagc tcccaacgga ttttaagtcc gttgcgtctt cctttccgcc atcccggcaa
     5101 gtcattgaag gtaaatcact taaattattt cctctatctg ttattttctc tccggtgcca
     5161 aaatagtgcc aactttctgt tagcaattcc acacttttct ttttatgctc ctgggataga
     5221 tgtgcatacc ttagaaccat tgaaaatgtt ttatgtccta acaattcctg aatagtttta
     5281 aggtcaactc ctttcattac aagccaactt gcaaaagtat gtctcaaatc gtgaaatctg
     5341 aaatctttta tatttaattc cttaataagt ttattgaaca tatgggaaac atgatgttta
     5401 cttatatgtc cagtttctat aattttacta taaaatacaa actcatttaa agggtctctt
     5461 aatgtttccg cgtatctcaa agtttctaca actattttat tcataggaac tacctttttt
     5521 tctttatttt ttgttctcca tacagttatt gtttctgtat taaaatctat atctctccat
     5581 cttaaactta caatttctcc aagtctcatc cctgtattta atgcaataat tattatagat
     5641 ttcaaaggtt ctttagcaaa atttacaaga agatttgcct ctgtttctgt taaatatctt
     5701 atcctcatat tattttcttt gtaaagtttt atctttggag aatttgtatt taattttccc
     5761 cattcaactg ccttgttaaa tatcgtctta aggcatgtta attctctatt tatcgtcgca
     5821 tattttacgc cttcttctat acgtttcttt ttataatttt ctatatcaaa acttgttatt
     5881 tcacctatat ttttctctcc aaaaaattca ttcaaatgtt ttatacttat cgtatcccta
     5941 atccaagatg atttattact tttcgcataa ttatctaaaa acaaatcagc catttcttta
     6001 aatttaattt ctttgtcttc ttttatatca aaatatttct tttcaattat cattgtttgt
     6061 atttttgcaa gaaccttctc tgcaagtttt ttgctttttc ctattttctt cctatgtctt
     6121 ttaccattga aagtataatc tatccaccaa ttgccatttc tctcaaatac tctcattttg
     6181 tctccttttt gattataact tatatttcta aatttttcaa aatttttaac tcttctaccg
     6241 tatgctgttc aatccattta ttcagttttt caatatcaaa tcttaaaaat tttccaccaa
     6301 ctttaacata gggaatcctt ttttcattta cccatttata taaagttttt gatgatatac
     6361 ccaaatactg attggcttct tttatattta gcaatctttt tagtattttt tctgccattt
     6421 tttaaaaatt acctcaaaaa ttttttgggt aatttttcca ttatccattt aaaaatcttg
     6481 tttaattttt taaaaattct ctcatcaact atcatttttt atccaacttt tatcgggtta
     6541 agttttaaat tactttttct tttacctttc aattttggat ttttaccttc tctaaatgtc
     6601 cataaagaac aatttatact acgacatcct tttaactcaa cttaccagaa gataaattca
     6661 gggaataatt gatagacaag aaaagagaca
//
LOCUS       DAOSAT010000096         6546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_7534_length_6546_cov_5.560622, whole genome shotgun sequence.
ACCESSION   DAOSAT010000096 DAOSAT010000000
VERSION     DAOSAT010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7534_length_6546_cov_5.560622"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..592
                     /locus_tag="PLF90_07835"
     CDS             101..592
                     /locus_tag="PLF90_07835"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOK57222.1"
                     /translation="MKKILVFLGFMCMGLLAQSGKIGYVDIKKVFDNYDEAKKTEEKF
                     KKEIDEAQKNLDKLQDEVKKMQEDYEKKKSMMKPEEQTKKENEIKAKVQELSKKWMET
                     KQKLDDRGKELENQIFEDIKKAIADYAKKNGFSMVVDSRLILYGESAVDLTDEVIKLI
                     NKK"
     gene            589..1623
                     /gene="lpxD"
                     /locus_tag="PLF90_07840"
     CDS             589..1623
                     /gene="lpxD"
                     /locus_tag="PLF90_07840"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOK57223.1"
                     /translation="MNMKAKEVAKIIDGKVIGDENITITGVSGIKEAKQGDLTFIANK
                     KYKNLLYTTKASAVIVGPDINNSVSTTLIQVENPSIAFAKIMSLVGPEPIVFPSGIHP
                     TAIIGKNVFLGKDVSIQPYAVIEDYVKIGDRTVIGAGVYIGHYTEIGYDCLIYPKVVI
                     RERIKIGNRVIIHPGTVIGGDGFGFATVKGVHHKIPQIGTVEIGDDVEIGSNVTIDRA
                     RFDKTYIGNGVKIDNLVQIAHNVYIGDNSIIVAQVGISGSTVIGKNVIIAGQAGIIGH
                     ITVGDNAVIGGKAGVTKNVPPNTHVTGFPAREKWEDMRFQAYMRKIPELIEKIKELEE
                     KIEKLEKKNG"
     gene            1616..2935
                     /locus_tag="PLF90_07845"
     CDS             1616..2935
                     /locus_tag="PLF90_07845"
                     /inference="COORDINATES: protein motif:HMM:NF009667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOK57224.1"
                     /translation="MDKFQKTIEKEVGIDGVGLHTGEKSRIILKPAPPNTGIIFIRKD
                     IEDSEPIKVDIENLLDTNKFPRRTSIGNEKLQIHTIEHLLSALYAMEIDNIFVEIYGS
                     ECPGLDGSCVGFVDLIKKGGIREYQEEKDVFYLKEPIYISENSSHIIALPSKEFRISY
                     TLDYPNTLINSQFASFVINPEIYEKEISPARTFCLKEEIDVLRNMGLGKGSTYQNTLI
                     IDKDKVINNEFRFQDEPVRHKISDLIGDIALLGKYLFAHIIGIKSGHSINTKLIRKIK
                     ELIRKEKISGVASPVYATVSSTQLEIEDIEKVIPHRYPFLLIDKILEINENKAVGIKN
                     VTINEWFFEGHFPGKPIMPGVLIIEAMAQVGGVLMLSKEENKGKLAYFMSVDRVKFRR
                     TVRPGDQLILEVEVVKLKSKVGQIQGRAYVDGKIAAEALLMFTVVEP"
     gene            2937..3716
                     /gene="lpxA"
                     /locus_tag="PLF90_07850"
     CDS             2937..3716
                     /gene="lpxA"
                     /locus_tag="PLF90_07850"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOK57225.1"
                     /translation="MKRIHPTAIISPEVEIGEDVEIGPYAIIEGNVKIRNGCVIGPFV
                     HIQGYTEIGENNRIFTGAIVGSIPQDLKYKGEITYLRIGKNNTIREFVTINPGTSKGE
                     ETRIGDNNLIMAYCHIAHNCIIKNNVIMANYAALAGHVIVDDYAIIGGLTGVHQFCKI
                     GKHSIIGGASKVTMDILPYIIADGHPAKPCGINVVGLRRRNFPKEKIEILEKAYKIIF
                     RSGLNTSDALEKLEEINNCEEIKDIIEFIKTSERGIAKERE"
     gene            3717..4490
                     /gene="lpxI"
                     /locus_tag="PLF90_07855"
     CDS             3717..4490
                     /gene="lpxI"
                     /locus_tag="PLF90_07855"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HOK57226.1"
                     /translation="MKENLAILVSDDFFSEYSFLKLKEKYNLFPFSFSESEKFNDSFI
                     VKDANVPLFFEFLKKNNIKLIFFAGKVKANLVFENLHPTAKNLLSQIGKLKPEDTMKK
                     IADFLESYGLKIIPQTDVFIDEIAENKVYTFPLTDEEMEDVKTGLSVLEDILKHRIGQ
                     SVIVKNGMILGVEGIEGTDELIKRIGKYCKDFVFVKGCNEDKDTRFDLPTIGMETVKN
                     MKMAGGKIIAVKSDKTIILEKDKVVEECMKNEIKLIGIK"
     gene            4487..5920
                     /locus_tag="PLF90_07860"
     CDS             4487..5920
                     /locus_tag="PLF90_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOK57227.1"
                     /translation="MKIKENFDEIISYLEDSSNFRKGKADKVYIPETENEIFEILEIC
                     RKERVPITVSGGGTGTVAGRIPVSGYIISTESFNKIIDINKEKKIATLQAGVIVDNFL
                     KEIEKEGLFYPPFPTERTAFIGGNVSTNASGEYSFKFGPTRKYVKRIKMVLTSCDIIE
                     IKRGEIFEKDGFIDYGIFKVPLPSYRTPDVKCSAGYYSKDGMDGIDLIIGSEGTLGII
                     TEVDVSLIENLPPRFIMILFLKDEENIPEIVREIKERKDELEIFSFEFFDKKSLLFLK
                     DDFNFIPEDTCAIYIEATNDTEKMEKWIEIAEKIGVLDTVIGEDITNYKKLIDFRHKL
                     PENVNAYFKKIGSIKIAVDAAVPEDKFENFYRFYRKIMEENKDIHTILFGHIGENHLH
                     FNLFPVDEKQKERAYKIYEESVKKAVYLGGTGFAEHGIGKLKHKYLEIMYGRDGILDM
                     VKIKKIFDPYCILGLDNIFPKEYLSLV"
     gene            6103..6369
                     /locus_tag="PLF90_07865"
     CDS             6103..6369
                     /locus_tag="PLF90_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOK57228.1"
                     /translation="MYYVYVIKSFKNSRRYVGFTSKNPVERLKEHNRGSNKWSRQNRP
                     FELIYYEEFTEKEKARKRERFLKSGKGREFLNKIIKNSNKTFKN"
     gene            6417..6488
                     /locus_tag="PLF90_07870"
     tRNA            6417..6488
                     /locus_tag="PLF90_07870"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6449..6451,aa:Asn,seq:gtt)
BASE COUNT         2620 a          728 c         1274 g         1924 t
ORIGIN      
        1 tatattacgg ttatgcactt gatgctgaag aaggagaacc gaaaggcaga attcatatag
       61 gaatgagttt tggtttttaa aaagaaagga ggaaagtaaa gtgaaaaaga ttttagtttt
      121 tttgggtttt atgtgtatgg gattgctggc tcagagcggg aaaataggat atgtggatat
      181 taagaaggtt tttgataatt atgatgaagc aaagaaaaca gaagagaagt ttaagaagga
      241 gattgacgaa gcacagaaaa atcttgacaa attgcaggat gaagtaaaaa aaatgcagga
      301 ggattatgaa aaaaagaaaa gcatgatgaa accggaagag cagacaaaaa aggagaatga
      361 gataaaggca aaagtacagg aattatcaaa gaaatggatg gaaacaaagc aaaaacttga
      421 tgatagggga aaagaacttg aaaatcagat atttgaagat atcaaaaaag caatagctga
      481 ctatgcaaag aagaatggtt tttctatggt agttgactca agattgattc tttacggaga
      541 aagtgcggtt gatttaactg atgaagttat aaaactcata aataaaaaat gaatatgaaa
      601 gctaaagaag ttgcaaaaat aatagatgga aaagttatag gtgatgaaaa tataactatt
      661 acaggtgttt ccggtataaa agaagcaaaa caaggggatt taacttttat agcaaacaaa
      721 aaatataaaa atcttcttta cacgacaaag gcatctgctg taatagttgg acctgatata
      781 aataattctg taagtacaac tttaattcag gtagaaaatc cttcaattgc ttttgcaaaa
      841 attatgtctc ttgtaggtcc agaacctata gtttttcctt ctggtatcca tccaacagca
      901 ataataggaa aaaatgtttt tcttggaaag gatgtatcaa ttcaacccta tgcagtaata
      961 gaggattatg taaaaatagg tgatagaact gtaatcggag caggtgttta catcggacat
     1021 tatacagaaa ttggctatga ttgtttgatt tatccaaaag ttgttataag agaaaggatt
     1081 aaaataggga atagagtcat aatacatcct ggaacagtta taggtggaga tggatttggt
     1141 tttgcaacag tcaaaggagt tcatcataaa attcctcaaa taggaactgt tgaaataggc
     1201 gatgatgttg aaattggttc aaatgttaca attgatagag caagatttga taagacatac
     1261 ataggaaatg gagtgaaaat agataacctt gtccagattg cccataatgt ttatataggt
     1321 gataattcaa tcattgttgc tcaggtgggt atttctggaa gtactgtaat aggtaaaaat
     1381 gtaataattg caggtcaggc aggtataata gggcatataa cagttggaga taatgcagta
     1441 ataggaggta aagcaggggt tacaaaaaat gttcctccaa atacccatgt tacaggtttt
     1501 cctgcaagag aaaaatggga agatatgaga tttcaggcat atatgagaaa gattcctgaa
     1561 ttaatagaga aaatcaaaga acttgaagaa aaaattgaaa aattagaaaa gaaaaatgga
     1621 taaatttcag aaaacgattg aaaaagaagt gggtattgat ggggtgggac tccatacagg
     1681 tgaaaaaagc aggattattt taaaacctgc tccaccaaat acaggtatta tatttataag
     1741 aaaagacata gaagatagtg aaccaataaa agttgatata gaaaatctcc ttgatacaaa
     1801 caaattccca agaagaacat caattggtaa tgaaaaactt caaatccata caatagaaca
     1861 tttactttca gcactttatg ccatggagat tgataatatt tttgttgaaa tttatggaag
     1921 tgagtgtccc ggtcttgatg gaagttgtgt tggatttgtt gatttgataa aaaagggagg
     1981 aataagggaa tatcaggaag aaaaagatgt attttattta aaggagccaa tttacatttc
     2041 agaaaacagt tctcatataa ttgctcttcc atctaaggaa ttcaggattt cctatacact
     2101 tgattatcca aatactctta taaattctca attcgcctca tttgttatta atccagaaat
     2161 ctatgaaaaa gaaatatctc ctgcaaggac cttttgtctt aaagaagaaa ttgatgtatt
     2221 gagaaatatg ggactgggta aaggttctac atatcaaaat acactaatta ttgataaaga
     2281 taaagttata aacaacgaat tccgatttca ggatgaaccg gtaagacata aaatttctga
     2341 cctgatagga gatattgcct tgcttgggaa atatcttttt gcacatatta tcggaattaa
     2401 gagtggtcat tcaataaata caaaattaat aagaaaaata aaagaactta taagaaaaga
     2461 aaaaataagt ggagttgctt ctcctgttta tgctaccgtt tcaagtactc aacttgaaat
     2521 tgaagatata gaaaaagtaa ttcctcatag atatcctttc ttactcattg ataaaatact
     2581 ggaaattaac gaaaataaag cagttggtat aaaaaatgtc accataaatg aatggttttt
     2641 tgaaggacat ttccctggta aacctattat gcccggtgtt ttaataattg aagcgatggc
     2701 gcaggttggt ggtgttctga tgttaagtaa agaagaaaat aaagggaaac ttgcctattt
     2761 tatgagtgtt gatagagtaa aatttagaag gacagtaaga ccgggagacc agttaattct
     2821 tgaagttgag gttgtaaaat taaagagtaa ggttgggcag attcagggca gggcttatgt
     2881 agatggtaaa attgccgcag aagcactttt aatgtttaca gtggttgagc cataaaatga
     2941 aaagaatcca tccaacagca attatatcac cagaggttga aattggtgaa gatgttgaaa
     3001 taggtcctta tgcaattatt gaaggaaatg ttaaaatcag aaatggatgt gtaataggtc
     3061 cttttgttca cattcagggt tatacagaaa taggagaaaa taacagaatt tttacaggag
     3121 caatagttgg aagtatacca caggatttga aatataaagg agagataaca tatttaagaa
     3181 tcggcaaaaa caatacaata agagaatttg taacaataaa tcctggaact tcaaaaggag
     3241 aagaaaccag aattggagac aataatttaa taatggctta ctgtcatatt gctcataact
     3301 gtataatcaa aaataatgtt ataatggcaa attatgctgc tttagcagga catgtaatag
     3361 ttgatgacta tgccattata ggtggactta caggtgttca tcaattctgt aaaataggca
     3421 aacactcaat aataggtggt gcttcaaaag ttacaatgga tatattacct tatattatag
     3481 cagatggtca tcctgcaaaa ccctgtggaa taaatgttgt cggacttaga agaagaaatt
     3541 tcccaaagga gaaaatagaa attctggaaa aagcatacaa aattattttc aggtctggat
     3601 tgaatacttc tgatgcattg gaaaaacttg aagaaataaa caattgtgaa gaaataaaag
     3661 atataattga atttataaag acatctgaaa gaggaattgc aaaagaaaga gaataaatga
     3721 aagaaaatct tgcaattctg gtaagtgatg attttttttc tgaatattca tttttaaaat
     3781 taaaagaaaa atataattta ttcccctttt ccttttcaga atcagaaaaa tttaatgatt
     3841 catttattgt aaaagatgca aatgtccctt tattttttga atttttgaag aaaaacaata
     3901 ttaaactcat tttttttgca ggaaaggtta aggcgaattt agtttttgaa aatttacatc
     3961 caacagcaaa aaatcttcta tctcaaatag gaaaattgaa accagaggat accatgaaaa
     4021 aaattgctga ttttcttgaa tcttatggac ttaaaataat tcctcagaca gatgttttta
     4081 tagatgaaat tgcagagaat aaagtttata cattcccgct tacagatgaa gaaatggagg
     4141 atgtaaaaac aggtctttct gttcttgaag atattttaaa acacagaata ggacagtcag
     4201 ttatagtgaa aaacggtatg attcttggag ttgaaggaat agaaggaaca gatgaactga
     4261 taaaaagaat agggaaatac tgtaaagatt ttgtttttgt aaaaggttgc aatgaagata
     4321 aagatacaag atttgattta ccgacaatag ggatggaaac agtaaaaaat atgaaaatgg
     4381 caggtggaaa aattattgct gttaagtcag acaaaacgat tatacttgaa aaagataaag
     4441 ttgtagaaga atgtatgaaa aatgaaataa aattaattgg aataaaatga agataaaaga
     4501 aaattttgat gagattattt cctatcttga agattcttct aattttagaa aaggtaaagc
     4561 agataaagta tatattcctg aaacagaaaa tgaaattttt gagattcttg agatatgcag
     4621 aaaagaaaga gttcctataa cagtttcagg gggagggaca ggaacagttg caggaagaat
     4681 tcctgtttct ggatatataa tttcaaccga aagttttaat aaaataattg atataaataa
     4741 agaaaaaaaa attgcgactt tgcaggcagg tgtaattgtt gataattttt taaaagaaat
     4801 tgaaaaggaa gggctttttt atcctccatt tcccacagaa agaactgctt ttattggtgg
     4861 gaacgtttca acaaatgctt caggtgaata cagttttaaa tttggaccaa caagaaaata
     4921 tgtaaaaaga ataaaaatgg tacttacttc ctgtgatatt attgaaataa aaagaggaga
     4981 aatttttgaa aaggatggat ttattgatta tggaattttt aaagtacctc tgccatctta
     5041 tagaacccct gatgttaaat gctctgcagg ttattattca aaagatggga tggatggtat
     5101 tgatttaatt attggttcag agggtactct cggaataatt acagaagtag atgtatccct
     5161 aattgaaaac ttaccgccga gatttattat gattttattt ttaaaagacg aagagaatat
     5221 tccagaaatc gtaagggaga taaaagaaag gaaagatgaa cttgaaattt tttcatttga
     5281 attttttgat aaaaaatctc ttcttttttt gaaagatgac tttaacttta tacctgaaga
     5341 tacatgtgct atttacattg aagcaacaaa tgatacagaa aaaatggaaa aatggattga
     5401 aatagcagaa aaaattggag ttttagatac agtaatagga gaggacatta caaactataa
     5461 aaaactcatt gatttcagac ataaacttcc tgaaaatgtt aatgcttatt ttaaaaaaat
     5521 aggaagtata aaaattgcgg ttgatgcagc agttccagag gataaatttg aaaactttta
     5581 tagattttac agaaaaatta tggaagaaaa caaagatatt catacaatac tttttggaca
     5641 tataggagaa aaccatctac attttaatct ttttccagtt gatgaaaaac aaaaagagag
     5701 agcatataaa atttatgaag aatcagtaaa aaaagctgtt tatcttggtg gaactggttt
     5761 tgctgaacac ggaataggta aattaaaaca taaatatctt gaaataatgt atggaaggga
     5821 tggaatttta gatatggtaa aaataaaaaa aatatttgac ccttactgca ttttaggtct
     5881 tgacaatatt tttccaaaag aataccttag ccttgtctaa cttctgaaat cttgacaatt
     5941 ttctaaaaat cccttattta tttttataaa aacttgataa aaatttttaa aaatagtaaa
     6001 ataaaatagt gttccggtgt agctcaacgg tagagcatcc gccactaaaa cattggcgga
     6061 cccgccaagt agtttggcgg gggcggctgt taactattga aaatgtatta tgtatatgtg
     6121 ataaaaagct ttaaaaatag tagaaggtat gtaggtttta catctaaaaa tccagtagag
     6181 agattaaagg aacataatag aggaagcaat aagtggagta ggcaaaatag accatttgaa
     6241 ttaatatatt atgaagaatt tactgaaaaa gaaaaagcaa ggaaaagaga gagatttctt
     6301 aaaagtggca aaggaagaga atttttaaat aaaataataa aaaattcaaa taaaactttt
     6361 aaaaattaaa caaaaaatat agaagaattg aaaaaattgt ggtaaaatta tatagttccg
     6421 gtgtagctca acggtagagc aggcggctgt taaccgcaag gttgtaggtt cgaatcctac
     6481 caccggagct atttataaat tttttttgtg gaagaaagta aaattggaat ttgaggatgt
     6541 aataaa
//
LOCUS       DAOSAT010000097         6326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_7851_length_6326_cov_4.367724, whole genome shotgun sequence.
ACCESSION   DAOSAT010000097 DAOSAT010000000
VERSION     DAOSAT010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7851_length_6326_cov_4.367724"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..116)
                     /locus_tag="PLF90_07875"
     tRNA            complement(43..116)
                     /locus_tag="PLF90_07875"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(80..82),aa:Arg,seq:gcg)
     gene            complement(126..662)
                     /locus_tag="PLF90_07880"
     CDS             complement(126..662)
                     /locus_tag="PLF90_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOK57229.1"
                     /translation="MKKIYFPLKNEKDLKKLKAGDYVLINGKILTARDAAHKKLIEII
                     NLKKNIPIEIKNELIYYTGPSPTPEGKIVGSIGPTTSERMDNLTIPLLKLGLKGTMGK
                     GERGKEIVKAFKRFKAVYFITYGGCGAYLSQFVKGIKLLAFEELGPEAIYQLEVYEFP
                     AIVGIDIYGDNFFNYRKV"
     gene            complement(659..1498)
                     /locus_tag="PLF90_07885"
     CDS             complement(659..1498)
                     /locus_tag="PLF90_07885"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOK57230.1"
                     /translation="MRKIFSEEIVNRICETIIEINYKLPEEVKKYIKIAYENEKEKYS
                     RKYLEIILKNIEIAERERIPLCQDTGLAIIFAEIGINVKIEKGIYKDLTEIINKGVEK
                     GSKKGFLRNSVVSAIERENTGNNTPCIIHYLVNEGDKLKFTIIAKGFGSENKSRLKML
                     NPSEGKMGIENFIIETVEKAGSSPCPPVFIGVGIGGSFEKAALLSKYALSKIGDENSK
                     YRDWEKEILFKINKLKIGPSGFGGNVTALDLKIETYPTHIAGLPVAVNLCCWAHRMGS
                     FEI"
     gene            complement(1467..2216)
                     /gene="recO"
                     /locus_tag="PLF90_07890"
     CDS             complement(1467..2216)
                     /gene="recO"
                     /locus_tag="PLF90_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOK57231.1"
                     /translation="MPPILIEGFILKKINFRETSVILTLFTKEFGKIKGVLKGVRKEK
                     SKIPPLTFTEGAYISTFLYKKRSELNLLSSPNLINFFYFEDKKSSKIYYYILKLIDLF
                     TPELQKDENIFYLLKETIENLKQNKKKHLIFLTFKIKFIEFLGYGIKIDNCCICEKES
                     RRYFFSPRKGGVLCYNCRKEDQNCVKISIRLISIIRFIKRNDFKNVGILNVKRNDAER
                     INFLCNLVLYYHSNLNFIWWRNEKNIFRGNC"
     gene            complement(2218..2775)
                     /locus_tag="PLF90_07895"
     CDS             complement(2218..2775)
                     /locus_tag="PLF90_07895"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOK57232.1"
                     /translation="MKTLKKHFISGIIFIVPISLSIWILFKIFIFLENILGNFFKKFF
                     VNIYTPGIGLISLILVILFVGFLANNFLGKKFLGLIELLFENIPFLNKIFSFIKNIVK
                     KITEEKKNIFKGVVKVRLPNDSYTIGFITDEGKDSKEICVFVPTVPNISTGIIFMIPK
                     EKIEKIDISVEDALKIVISMGIFKS"
     gene            complement(2779..3495)
                     /locus_tag="PLF90_07900"
     CDS             complement(2779..3495)
                     /locus_tag="PLF90_07900"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HOK57233.1"
                     /translation="MKNKKNSSLLESFNSAFNGLIYVFKRERNFKIHVFMGIVAILLS
                     LFFHVPFTETLIIIVLISLVMVSEIINTGIEILSENMSKENQNIIKVVKDICASAVLV
                     SSIFAFICGYLIFIKFFPEGFRNIFENIARSPWYFTFITLIIVSFLTIVLKILTGESF
                     TLAGGMPSVHSGIAFSIWVAISFLTFKDYPVISPLVFLLSFWVAQSRITKKIHTIEEV
                     IIGGIIGIIVTILLFQIFWR"
     gene            complement(3464..3925)
                     /gene="ybeY"
                     /locus_tag="PLF90_07905"
     CDS             complement(3464..3925)
                     /gene="ybeY"
                     /locus_tag="PLF90_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOK57234.1"
                     /translation="MRNTTKIEMEIEIFQRIRKIKINKEFLKNKLIRLKKIINPPPKK
                     VFVYLVNNRKIKLLNKEFLKKNGFTDVISFKYSKDFGELIISVEECEKNAKIYSNTLE
                     EELLYVIIHGILHLKGYRDYNKKEREKMLSIQDEIFSVIMQNEKQKKFFFA"
     gene            complement(3855..4865)
                     /locus_tag="PLF90_07910"
     CDS             complement(3855..4865)
                     /locus_tag="PLF90_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOK57235.1"
                     /translation="MIIKKQIEISNEEAQFLYGIRDENLEFIENLFGIEIFARGNLLT
                     FKGENEKVEKVLELLHEILRNRAKKKFITKSDILMMVRDDKGKEIKDEKTQEKKDSSI
                     WINSKKDFVIPKTSTQKKYVKAIRKYDLVVGIGPAGTGKTYLAVACGIEAIKKGQFQR
                     FILTRPALEAGERLGFLPGDLEEKIKPYLQPIYDALFDLLKYEELRRWQERKIIEIVP
                     LAYMRGRTLSNAFIILDEAQNTSFAQMKMFLTRLGIDSKIVITGDITQIDHPLSSTTS
                     GLVEVQSILSGIKGIKFVYFTNRDVVRHKLVKKIIDAYEKYYKNRNGNRNLSEDKENK
                     NK"
     gene            complement(4868..5617)
                     /locus_tag="PLF90_07915"
     CDS             complement(4868..5617)
                     /locus_tag="PLF90_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOK57236.1"
                     /translation="MKKLLLFVCSLLLSGCAMYQTKREIEPKKSFEEMVDIKLKQAIA
                     IAETALEISKNAEKLSIEAVNTSNQAKANSEVAIKKVDEAIAATNEVKKFTESEVQKA
                     ISAANKASEEAIKAANEASQKAIDKSNEAIKIANEASEKAINMANEASEKAIKASNEA
                     SEKSIAVANQTIAEINKLRATIQMKPSEPIIEEEPKSGKYYTIQKGDTLKKIAYKFYN
                     DTSKWYLIYQANKDIIKNPDNLVPGRKIYIP"
     gene            complement(5653..>6326)
                     /locus_tag="PLF90_07920"
     CDS             complement(5653..>6326)
                     /locus_tag="PLF90_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57237.1"
                     /translation="PLKKILSENIVNEIKNLSPHKSSNELLLFLGGKRSWVNEILKGL
                     IDRMKDKLIEKKDELSFIWVVDFPLFEYNEEEKRIQSVHHPFTSPKIEDIEILETEPL
                     KVKSRAYDIVLNGIEIGGGSIRINKRELQEKIFKIIGLEENIYLQRFGFLLEALEFGA
                     PPHGGIAIGLDRLVMLLLGEESIRETIAFPKTQKGVCLLTDAPGEVEYNLLKENRIKI
                     DIANE"
BASE COUNT         2039 a         1114 c          678 g         2495 t
ORIGIN      
        1 cgaaccaagt gctctaccag gctgagccac accccgcaaa tatgcggcca gcccgacttg
       61 aacgggcaac tctcgggtcc gcaacccgat gttctatcca tttgaactat ggccgctgat
      121 ataaattata cctttctata attaaagaaa ttatctccat aaatatcaat cccaactatt
      181 gcaggaaatt catatacttc aagttgataa attgcttctg gtcctaattc ttcaaaagcc
      241 aacaacttta ttcctttcac aaattgactt aaataagcac cacacccacc ataagtaata
      301 aaataaaccg ctttaaatct tttaaatgct tttactattt cttttcctct ttctcctttt
      361 cccatagttc cttttaaacc aagttttaac aaagggattg tcagattatc cattctctca
      421 ctcgttgtag gaccaattga accaactatt tttccctcag gagtaggaga aggacctgta
      481 taataaatta attcattttt tatctctata ggaatatttt ttttcaagtt tataatttca
      541 atcaattttt tatgtgccgc atcccttgct gttaaaattt tcccatttat taaaacataa
      601 tctcctgctt taagtttttt taaatctttt tcatttttca atgggaaata tatttttttc
      661 atatttcaaa actccccatt ctgtgagccc aacagcataa atttacagca acaggcaaac
      721 ctgctatatg agttggataa gtttcaattt ttaaatcaag tgctgtaaca ttaccaccaa
      781 atcctgaagg tcctattttt aacttgttta ttttaaaaag aatttctttc tcccaatctc
      841 tgtatttgga attttcatct ccaattttac tcaaagcata ttttgaaagc aacgctgctt
      901 tctcaaaaga accaccaatt ccaacaccaa taaaaacagg tggacaggga gaactacctg
      961 ctttttccac tgtttcaatt ataaaatttt ctatacccat ttttccttct gagggattaa
     1021 gcatctttaa tctgctttta ttctctgaac cgaaaccctt tgcaattatt gtaaacttta
     1081 atttatcacc ctcattcacc aagtaatgaa taatacatgg agtattattt cctgtattct
     1141 ctctttctat tgcactcacc acagaattcc ttaaaaaacc ttttttactc cctttttcaa
     1201 cccctttgtt tattatctct gtaaggtctt tatatatacc tttctcaatt tttacattta
     1261 tccctatttc tgcaaatata attgcaagtc cagtatcctg acataaagga attctttctc
     1321 tttcagcaat ttcaatattt ttaagtatga tttctaaata ttttctggaa tacttctctt
     1381 tttcgttttc ataagcaatt ttaatatatt ttttcacctc ttcaggcagt ttataattaa
     1441 tctctataat tgtttcacat attctattaa caatttcctc tgaaaatatt tttctcattt
     1501 ctccaccata taaaatttaa atttgaatga taataaagaa caagattaca caaaaaattt
     1561 attctttcag catcatttct cttgacattc agtattccaa catttttaaa atcatttctt
     1621 ttaataaatc ttattattga aattaatcta atagatattt taacacaatt ctggtcttct
     1681 tttctacaat tataacaaag aacaccccct tttctcggag aaaaaaaata tcttctactt
     1741 tctttttcac aaatacaaca attatcaatt tttattccgt atccaagaaa ttctataaac
     1801 tttattttaa aagtaagaaa aattaaatgt ttttttttat tctgttttaa attttctatt
     1861 gtttctttca aaagataaaa aatattctca tctttctgta actctggagt aaaaaggtct
     1921 ataagtttta aaatatagta gtatatttta gaggattttt tatcctcaaa ataaaaaaaa
     1981 ttgattaaat ttggagaact taaaaggttt aactctgatc tctttttata aagaaatgtt
     2041 gaaatatatg cgccttcagt aaaagttaaa ggaggaattt tgcttttttc ttttctgaca
     2101 ccttttaaaa ctcctttaat tttcccaaat tcttttgtaa ataaagtcag tattacactc
     2161 gtttctctaa agttaatttt ttttaaaata aaaccttcaa tcaatattgg tggcatttta
     2221 agacttaaaa atacccatag agataacaat ttttaaagca tcttcaactg aaatatctat
     2281 tttttcaatt ttttcttttg gaatcataaa aataattcct gtacttatat tcggaacagt
     2341 aggaacaaaa acacatattt ctttactatc tttcccttca tctgtaatga atccaattgt
     2401 atatgaatcg ttaggtaacc taactttaac cactcctttg aaaatatttt ttttctcttc
     2461 tgttattttt ttaacaatat tttttataaa actgaaaatt ttattcaaaa atggaatatt
     2521 ttcaaaaagt aattcaatca atccaagaaa cttttttcct agaaaattat ttgccagaaa
     2581 acctacaaat aaaatcacaa gtatcagaga gattaaacca attccaggcg tatatatgtt
     2641 gacaaaaaat tttttaaaaa aatttccaag gatattctca agaaaaataa atattttaaa
     2701 taaaatccat atagaaagag agataggaac tataaaaatt atccctgaaa taaaatgttt
     2761 ttttaatgtc ttcaattttc acctccagaa aatttgaaaa agtaatatag taacaattat
     2821 acctattatt cccccaatta taacctcttc tatggtatga attttttttg ttattctgct
     2881 ttgagcaacc cagaaagaaa gtaaaaaaac aagtggagaa attacagggt aatccttaaa
     2941 tgttaaaaag gaaatagcaa cccatatact aaaagcaatt ccgctatgaa cactcggcat
     3001 accacctgca agagtaaaac tttctcctgt aagtattttc aaaacaatag ttagaaatga
     3061 aacaataata agagtaataa aagtgaaata ccatggagac ctcgcaatat tttcaaaaat
     3121 atttctaaaa ccttctggaa aaaactttat aaagattaaa taaccacata taaaagcaaa
     3181 aattgaagat actaaaacag cggaagcaca aatatcttta acaactttta taatattctg
     3241 attttcctta ctcatattct ctgaaagaat ctctataccg gtatttatta tttcggaaac
     3301 cattacaaga gaaataagaa cgataattat taaggtttca gtaaatggca catgaaaaaa
     3361 aagagataat aatattgcaa caatacccat aaaaacatga attttaaaat ttctttctct
     3421 tttaaaaaca taaataagtc cattaaaagc agaattaaaa ctttcaagca aagaagaatt
     3481 ttttttgttt ttcattttgc ataatcacac taaaaatttc atcctgaata gacaacattt
     3541 tttctctttc ttttttatta taatctctat atccttttaa atgtaaaatc ccgtgaatta
     3601 ttacataaag aagttcttcc tccagtgtat tggaataaat ttttgcattt ttttcacatt
     3661 cttcaacaga aattattaat tctccaaagt cctttgagta tttaaaagaa ataacatcag
     3721 taaatccatt tttttttaga aattctttat ttaacaattt tatttttctg ttattaacta
     3781 aataaacaaa tacttttttt ggtggtggat taattatttt ttttaacctg ataagtttat
     3841 tttttaaaaa ttctttattt atttttattt tccttatcct ctgaaagatt tctatttcca
     3901 tttctatttt tgtagtattt ctcataagca tctatgattt ttttgacaag tttatgtctt
     3961 acaacatctc tattggtaaa gtaaacaaat ttaatccctt ttatccctga aagaatactt
     4021 tgaacttcta caagccctga agttgttgaa gataaaggat ggtcaatctg ggttatatca
     4081 cctgttataa caatttttga atcaatacca agtcgtgtta aaaacatttt catttgtgca
     4141 aaactcgtat tttgtgcttc atcaagtatt ataaaagcat tacttaaagt tctacctctc
     4201 atatatgcaa gtggaacaat ttctataatt tttctttcct gccatcttct caactcttca
     4261 tatttaagca ggtcaaaaag tgcatcatat ataggttgaa ggtacggttt aatcttttct
     4321 tcaagatcac ctggcaaaaa tccaagtcgt tcccctgctt caagagcagg tctggttaaa
     4381 ataaatctct gaaactgtcc ttttttaatc gcttcaattc cacatgcaac tgcaagatat
     4441 gtttttcctg ttcctgcagg tcctattcca acaacaaggt catattttct tattgctttt
     4501 acatattttt tttgagtgga tgtttttggt ataacaaaat ctttttttga atttatccag
     4561 atactactgt cttttttttc ctgtgttttt tcatctttta tttcttttcc tttatcatct
     4621 cttaccatca taagaatatc actttttgta ataaatttct tttttgctct atttctaagg
     4681 atttcatgta aaagttctaa aactttttct actttttcat tttccccttt aaatgtcagt
     4741 aaattccctc tggcaaaaat ttcaatccca aaaagatttt ctataaattc aagattttca
     4801 tctcttatac cataaaggaa ttgggcttct tcgttggaaa tttcaatctg ttttttaata
     4861 atcattttta gggtatgtat atttttctac cgggtactaa attatcagga ttttttataa
     4921 tgtctttatt tgcttgataa atcaaatacc attttgaagt atcattataa aacttataag
     4981 caattttttt taaagtatct cctttttgaa tggtataata tttacctgat ttaggttctt
     5041 cttctataat tggttccgat ggtttcatct gaatagttgc tctaagttta ttaatttctg
     5101 ctattgtttg attggctact gcaattgatt tttcacttgc ttcattggat gcttttattg
     5161 ctttttcact tgcttcattt gccatattta ttgctttctc acttgcttca tttgctattt
     5221 ttattgcttc atttgactta tcaattgcct tttgagatgc ctcatttgca gccttaatcg
     5281 cctcttcaga tgctttattt gctgcactta ttgctttctg aacttcactt tcagtaaatt
     5341 ttttaacttc atttgttgct gctattgctt catctacttt ttttatcgct acttcagaat
     5401 ttgcctttgc ctgattactc gtattaactg cttctataga taatttttca gcatttttac
     5461 tgatttcaag tgctgtttcc gctattgcta ttgcctgttt taatttaata tcaaccattt
     5521 cctcaaaaga tttttttggt tcaatttctc ttttggtttg atacatcgca caaccactta
     5581 ataataaact acatacaaat aataacagtt ttttcatttt cccctccaca ttttcttata
     5641 aaaagattat cattattcat ttgcaatgtc aattttaatt ctattttcct ttaaaagatt
     5701 atattccact tcacccggtg catctgttaa aagacaaact cctttctggg tttttggaaa
     5761 agcaatagtt tcccgtatac tttcttctcc aagtaaaagc atcaccaacc tatcaagccc
     5821 gatagcaata ccgccgtggg gtggagcacc aaattcaagg gcttccagta aaaatccaaa
     5881 tctctgtaaa taaatattct cttcaagtcc tataatctta aaaatctttt cctgaagttc
     5941 tcttttattt attcttatac ttcctccacc aatttctata ccatttaata caatatcata
     6001 tgctcttgat tttaccttta acggttctgt ttcaagtatt tcaatatcct ctattttagg
     6061 agaagtaaaa ggatgatgaa ccgattgaat ccgtttttcc tcttcgttgt attcaaaaag
     6121 aggaaaatca acaacccata taaaagaaag ttcatctttt ttttcaatta atttatcttt
     6181 cattctatca atcagtcctt taagtatttc atttacccaa cttctttttc ctccaagaaa
     6241 taataaaagt tcattggatg atttatgagg tgataagttt ttaatttcat ttactatatt
     6301 ttcagaaaga atttttttga gtggac
//
LOCUS       DAOSAT010000098         6219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_8022_length_6219_cov_5.025308, whole genome shotgun sequence.
ACCESSION   DAOSAT010000098 DAOSAT010000000
VERSION     DAOSAT010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8022_length_6219_cov_5.025308"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLF90_07925"
     CDS             complement(<1..286)
                     /locus_tag="PLF90_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOK57238.1"
                     /translation="MTSRERVDRSVNFQEVDRVPIDLGGMKASGINVIAYDRLKRYLG
                     LKSPTKVLDARFMIAVVEEEIRKLFHIDVMPLDLENALWWATAKEKDWIPR"
     gene            complement(412..942)
                     /locus_tag="PLF90_07930"
     CDS             complement(412..942)
                     /locus_tag="PLF90_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOK57239.1"
                     /translation="MEFEDVIKTRRSIRSYNKKEIPEDILKKIFEGTRVAPSGNNKQP
                     WYFIVVKDKDKKSKIAKYSYNQSFIAEADCVVICCGKKYPNPYEPLKDNCYLVDVTIA
                     IDHLILTARNYGVGSCWVGAFYPEPIKKLLKIPQDIDILMIVPLGYTSSEFYESKNRK
                     DLKEFVFKEEFNKPFF"
     gene            complement(1073..2071)
                     /locus_tag="PLF90_07935"
     CDS             complement(1073..2071)
                     /locus_tag="PLF90_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOK57240.1"
                     /translation="MKLKRWSGNPILTPNPEIEWEKEAVYNPGAFLHQGKIYLLYRAV
                     GEYELYSSRFGLAISKDGLHFERVSDKPVFEPYANYDKGGCEDPRIVKIENEFFITYA
                     ALPNPPSKSGNFIEVMQKLKVDPLYPRIHVFSHTGLLSSFDLYNFKRMGIITPPEIDD
                     RDGVLFPEKIDGKYLLLHRPTEWIGEKFGTDKPSIWLAYSTDLKNWGENKLLLKPEYP
                     WEEKKIGSGPPPIKTEEGWLLIYHGVDKNFVYRTGVVLLDLNHPEKVIARSPYPILEP
                     EENYEKVGDIPNVVFPTGTVLIDGEIFVYYGAADKVCGVATTSLKDLLEYVLKFKV"
     gene            complement(2085..3410)
                     /locus_tag="PLF90_07940"
     CDS             complement(2085..3410)
                     /locus_tag="PLF90_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOK57241.1"
                     /translation="MEKKDIIILMPDQLRWDCLGAYGNKIIKTPNIDKIAEEGVIFKN
                     AYTTSPLCMPARASFVSGLYPHQHGMWENKGSLPPENETFFHHLQKNGWFVAHIGKAH
                     FYEHKNFHMKTMEDYMRKRGIDYVHETTGPHATVTTDSYMTDYLKEKGLLEIFRNDYR
                     NRNKFSTHPSPLKEEDYLDSYVGKKACEFIKNYSFEKPLCLFVGFPSPHEPWDPPGKY
                     AEMYSPEDMPLPVEEIIETADLPEKTKKIIEQWKRIFNGLSQEIIKKIRSLYYGKISL
                     VDYWIGEILKTYYEKRTPENLIIIFWSDHGEMLGDHGLLYKSVFYEQSVKIPLIIKWP
                     GKNDELRKSANKIVEIIDIFPTLLSGLGLEKSKRVVGENLFEGKKKNFALSEIYAFGF
                     HQYMIRTEKFKMVFENDGDCLFLFDMENDPDEITNLAGKIKYFKSDLIN"
     gene            complement(3478..4242)
                     /locus_tag="PLF90_07945"
     CDS             complement(3478..4242)
                     /locus_tag="PLF90_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK57242.1"
                     /translation="MKLGIVTYMIAAEWDVDTIIKKCEELGYEGVELRTQHKHKVESN
                     LTKKEREEVRKKFENSKVKLVGLGTIFEYHSPDKEILKKNIEGTKEYVILAKDVGAEG
                     IKVRPNAFPPNVPKEKTIEQIGKSLREVSSFASDYGIKIRLEVHGHETSNPVYIKQMV
                     DIADHPNLYVCWNSNMTDIDETGSIEKNFNLLKDKIEIVHINELCNDYPWLTLFKLLK
                     EINFKNFCLAEILSNPEPERLLRYYKALFNAYLKLV"
     gene            complement(4258..5277)
                     /locus_tag="PLF90_07950"
     CDS             complement(4258..5277)
                     /locus_tag="PLF90_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK57243.1"
                     /translation="MEKIKCAVIGTGIFGEIHVHTYKTYLKSELIKICDINEKRAKEI
                     SEQYDVKYTTDYKEIAQDKEIQAVSVVTPDFAHRDIVIEMLKNGKNVLVEKPMATNTK
                     DAEDMVNEAKRNKKILMTDFHNRFNPYFVQIKDSFDKGEIGNICSIYTRLSDRIDVPL
                     KWFSWSGKSGPHWFLFPHTVDLVCWFSKSLPEKIYAIGKKGVLSGYGIDTYDTVTAIL
                     DFGRFYATVETSWIIPESWPSIVDFYFKIEGSKGKLEVDLNEFVCKISSDIKRETNFP
                     MITGYLPIDGKPIGFVSLPIQHFIDCIYDNKEPFITLEDGLNNVKIIEKIVESVEKGK
                     TVFLK"
     gene            5380..>6219
                     /locus_tag="PLF90_07955"
     CDS             5380..>6219
                     /locus_tag="PLF90_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57244.1"
                     /translation="MRKNKNIEKIEKAIEFIGKFNNTKIIKEIAWNWNPFFDNYRYFD
                     LFNREYKGTMTRNGDIIVKVFPAKIEKMLNFAEGKKEYVIRGEKIYRKTDLEIVSNFI
                     LEFLKRRNFGVLNMRSLKIKNGKKIELVFDNHIGEIEVVRTKPFFEFRLKDKEPERPY
                     FCTPHHQYIFKVKNLIEMGKNEDILIDYEESEIATTSFSPFALIRRAIYGFGSYNLRG
                     FYSDEGLFPEVKHGIRQTVYGNLAGSIVGASVFVRVAERLKDNTWYTLASGILAFIRA
                     PAEI"
BASE COUNT         2006 a         1032 c          862 g         2319 t
ORIGIN      
        1 ttctgggaat ccaatctttt tcttttgccg ttgcccacca gagtgcattt tctaaatcaa
       61 gaggcataac atctatgtga aaaagttttc ttatttcttc ttccactaca gcaatcataa
      121 accttgcatc aagtactttt gtaggtgact ttaatcccag atatctttta aggcggtcat
      181 aagcaataac atttatccca cttgctttca ttccacctaa atctatagga acacggtcaa
      241 cttcttgaaa attcacactc ctgtctactc tttctcttga tgtcataaaa tccccctttt
      301 ataaataact tttacaataa gttaaaaaca taacacttaa tctgtcaaat ttttaattgt
      361 tccaatactt cgttagaggt aagaagtgag gtcatttaag agaaaatttt ttcaaaaaaa
      421 gggtttgtta aattcttctt taaaaacaaa ttctttaagg tcttttctat tcttactttc
      481 ataaaattca ctggaagtat atccaagagg aactatcatt aaaatatcta tatcctgagg
      541 tattttaagc agttttttta taggttcagg ataaaatgca ccaacccagc aactcccaac
      601 tccataattt ctcgcagtta aaataagatg gtcaattgca attgttacat ccacaagata
      661 acagttgtct tttaatggct catatggatt tggatatttc ttcccacaac aaataactac
      721 acaatctgcc tctgcaataa atgactgatt ataggaatat tttgctatct tacttttttt
      781 atctttatcc tttacaacaa tgaaatacca tggttgttta ttatttcctg atggagctac
      841 tctcgtacct tcaaaaattt ttttcaatat atcttccggg atttcctttt tgttgtaact
      901 tcttatactt cttcttgtct ttataacatc ttcaaattcc atttaaaacc tcctttttta
      961 aaatatattc cacaaaacca aaaaatgtca atttttatat tatgaaaaaa taactaaaag
     1021 gggtagatag atgaatgaaa aattaaagtt caatttaatt gttaaataac tttcaaactt
     1081 tgaattttaa tacatattct aaaaggtctt ttaatgaagt tgttgccact ccacatactt
     1141 tatctgctgc tccataataa acaaaaattt ctccatctat caaaactgta cctgtgggaa
     1201 aaacaacatt tggtatatct cctacttttt cataattttc ttctggttca agaataggat
     1261 agggtgaacg agcaataact ttttctggat ggtttaaatc taaaagaact actcctgtcc
     1321 tgtaaacaaa atttttatca acaccgtgat atataagaag ccatccttct tctgttttta
     1381 taggtggtgg acctgaacct atttttttct cttcccatgg atattctggt ttaagtagaa
     1441 gtttattttc accccagttt tttaaatctg ttgaataagc aagccagata cttggtttat
     1501 cagttccaaa cttttctcct atccattcag tgggtctgtg tagtaaaaga tattttccat
     1561 ctatcttttc tggaaaaaga acaccatcac ggtcatcaat ttcaggtgga gtaataattc
     1621 ccattctttt aaagttatat aaatcaaaag aagaaaggag tcctgtatga gaaaaaacat
     1681 gtattctggg ataaagaggg tcaactttaa gtttctgcat aacttcaata aaattacctg
     1741 atttagaggg gggattgggt aaagcagcat aggtgataaa aaactcgttc tctattttaa
     1801 caattcgtgg gtcttcgcaa ccgcctttat cataatttgc gtatggttca aaaactggtt
     1861 tatcagaaac tctttcaaag tgtaatccat ccttacttat tgccaatcca aatcttgaag
     1921 agtacaattc gtattcaccc actgctctat aaagtaagta aatttttccc tgatgtagaa
     1981 aagcccctgg attgtaaact gcttcttttt cccattcaat ttctggattc ggagtaagaa
     2041 taggattccc tgaccatctt tttaatttca ttagccctcc ttttttaatt tataaggtca
     2101 gatttaaaat atttaatttt tcccgcaaga tttgtaattt catcagggtc attttccata
     2161 tcaaaaagaa ataggcaatc tccatcattt tcaaaaacca ttttaaattt ttcagttctt
     2221 atcatatact gatgaaaacc aaatgcataa atctcactca aagcaaaatt tttctttttc
     2281 ccttcaaata agttctcacc gactactctt tttgattttt caagcccaag acctgaaaga
     2341 agcgtaggga aaatatcaat tatttcaaca attttattag cactttttct caattcatca
     2401 tttttacctg gccactttat tattaaaggg atttttacac tctgttcata aaatacactt
     2461 ttataaagaa gtccatggtc tccaagcatt tctccatggt cagaccagaa aattattata
     2521 agattttcag gagttcgttt ttcataatat gtttttaaaa tttctccaat ccagtaatca
     2581 acaagagaaa ttttaccata ataaagagac cttatttttt ttattatttc ttgacttaaa
     2641 ccattaaaaa ttcttttcca ttgttctatt atttttttcg ttttttcagg caggtcagca
     2701 gtttctatga tttcttcaac aggtagtggc atatcttcag gagaatacat ttctgcatat
     2761 tttccaggtg gatcccatgg ttcgtgagga gaaggaaatc caacaaaaag acataaaggt
     2821 ttttcaaatg agtagttttt aataaattcg catgcttttt taccaacata actgtcaaga
     2881 tagtcctctt ctttaagtgg gcttggatga gttgaaaatt tatttctatt tctgtagtca
     2941 tttctgaata tctcaagaag tcctttttct ttcaggtaat ctgtcatata tgagtcagtt
     3001 gtgactgttg catgaggacc tgttgtttcg tggacataat caattcctct ttttctcata
     3061 taatcttcca ttgttttcat atggaaattt ttgtgctcat aaaaatgtgc ctttcctatg
     3121 tgagcaacaa accatccgtt tttctgtaga tgatgaaaga aagtttcatt ttcaggcgga
     3181 agagaacctt tgttttccca catcccgtgc tggtgagggt aaagtccact tacaaaagat
     3241 gctcttgcag gcatacaaag aggacttgtt gtataggcat ttttgaatat aaccccttct
     3301 tctgcaattt tatcaatatt gggtgttttt atgattttgt ttccataagc acctaaacag
     3361 tcccatctta actggtcagg cattaaaata attatatctt ttttttccat ttttaattct
     3421 ctttaaccat ttattattga cttcaaatac cggtgaaaaa ataattgaaa tcaatcttta
     3481 tacaagtttc aaatatgcgt tgaaaagtgc tttatagtat ctcaataatc tttctggctc
     3541 tggatttgac aatatctcag caagacagaa gtttttgaag tttatttctt taagtaattt
     3601 gaataatgtc aaccatggat aatcattaca gagttcattt atatgtacaa tctctatttt
     3661 atctttcaga agattaaaat ttttctcaat agaacctgtt tcatcaatat ctgtcatatt
     3721 tgaattccag catacataaa gatttggatg gtctgcaata tctaccatct gctttatata
     3781 tacagggttt gatgtttcat gtccatggac ttcaagccgg atttttatac cataatcaga
     3841 agcaaaagat gatacctctc ttaatgattt tcctatctgt tcaatagttt tttctttagg
     3901 aacattgggt ggaaaagcat ttggtcttac ttttattcct tctgctccta catcttttgc
     3961 aagtattaca tattcttttg ttccttcaat gtttttcttc aaaatttctt tatcaggact
     4021 gtgatattca aaaattgtcc caagtccaac aagtttaact tttgaatttt caaatttttt
     4081 ccttacttct tctctttctt tttttgtcag gtttgattct actttatgtt tgtgctgtgt
     4141 cctcaattcc actccttcat acccaagttc ttcacatttt tttataattg tatcaacatc
     4201 ccattcagca gcaatcatat aagttacaat tccaagtttc atttttattc ctcctttcta
     4261 ttttaaaaaa actgtttttc ctttttccac tgattcaact attttttcta ttattttaac
     4321 attattaaga ccatcttcca gtgttataaa aggttcttta ttatcatata tacaatcaat
     4381 aaaatgctga attggtagag aaacaaaacc aataggtttt ccatcaatag gtaaatatcc
     4441 tgttatcatg gggaaatttg tttctctttt tatatctgaa gatattttac atacaaattc
     4501 atttaaatca acttcaagtt tacctttact tccttcaatt ttaaaataaa aatcaacaat
     4561 agagggccag gattcaggaa taatccagga tgtctcaact gttgcataaa atcttccaaa
     4621 atctaaaatt gcagtaacag tatcataagt atcaatgcca tatcctgaaa gaactccttt
     4681 tttcccaatt gcataaattt tttctggaag agattttgag aaccagcaaa caaggtcaac
     4741 tgtatgagga aataaaaacc agtgaggacc tgatttacct gaccatgaaa accattttaa
     4801 aggaacatct attctatcac tcaatcttgt ataaattgaa caaatatttc caatttcacc
     4861 tttgtcaaaa ctatctttaa tttgaacaaa ataaggattg aatctgttat gaaaatccgt
     4921 catcaaaatt tttttatttc tttttgcctc atttaccata tcttctgcat ctttcgtatt
     4981 tgttgccatg ggtttttcta caagaacatt ttttccattt tttaacattt caataacaat
     5041 atctctatga gcaaaatcag gagtgacaac tgaaactgcc tgaatttctt tatcttgtgc
     5101 aatttcttta taatcagttg tatattttac atcatattgt tcacttatct cctttgccct
     5161 tttttcattt atatcacaga tttttataag ttcagattta agatatgttt tgtaagtatg
     5221 aacatgtatt tcaccaaaaa taccggttcc tataactgca catttaattt tttccatttt
     5281 ttgacccttc acattggttt aaatataaac tttctgttgt aatatgtcaa taagaaattt
     5341 aaacttgaca atttgtaaat aaatagaaaa ataaaaaata tgagaaaaaa taaaaatata
     5401 gaaaaaattg agaaagcaat agaatttatt ggaaaattta ataatacaaa gataattaag
     5461 gaaattgcat ggaactggaa tccttttttt gataattaca gatattttga cctttttaat
     5521 agagaatata aaggaacaat gacaagaaat ggtgatataa ttgttaaagt ttttcctgca
     5581 aagattgaaa agatgcttaa ttttgctgaa gggaagaaag aatatgtaat aagaggagaa
     5641 aaaatttaca gaaaaacaga tttagagata gtttctaatt ttatactgga atttttgaaa
     5701 agaaggaatt ttggagttct aaatatgagg tctttaaaga taaaaaatgg taaaaaaata
     5761 gaattagttt ttgataacca tataggagaa atagaagttg taagaacaaa gccatttttt
     5821 gaattcagat tgaaggacaa agaacctgaa agaccttatt tctgtactcc acatcatcag
     5881 tatatcttca aggttaaaaa tttaattgag atgggaaaaa atgaggatat tttaattgat
     5941 tatgaagaaa gtgaaattgc aacaacttct ttttctcctt ttgctttaat aagaagagca
     6001 atatatgggt ttggaagtta taatttaagg ggtttttatt ctgatgaagg cctttttcca
     6061 gaggttaaac atggaataag gcagacagtt tatggaaatc ttgcaggaag tattgttggc
     6121 gcaagtgttt ttgtaagggt tgctgaaaga ttgaaggata atacttggta tactcttgct
     6181 tctggaattc ttgcttttat aagggctcct gctgaaatt
//
LOCUS       DAOSAT010000099         5937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_8503_length_5937_cov_3.382013, whole genome shotgun sequence.
ACCESSION   DAOSAT010000099 DAOSAT010000000
VERSION     DAOSAT010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8503_length_5937_cov_3.382013"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /gene="folP"
                     /locus_tag="PLF90_07960"
     CDS             <1..494
                     /gene="folP"
                     /locus_tag="PLF90_07960"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOK57245.1"
                     /translation="SALEMDRKLVDILKTSECGYVLMHMKGTPKIMQENPYYKDTIGE
                     ITEFFKGKLEYIEKNGIDIERVVIDPGIGFGKRVIDNILILKHLDKFKKFKRPIFIGT
                     SRKAFIGKILNLEVEQRLEGSLATVVYAYLKGARIFRVHDVKETKRVLDIIQAIENEN
                     IGT"
     gene            475..918
                     /gene="ruvX"
                     /locus_tag="PLF90_07965"
     CDS             475..918
                     /gene="ruvX"
                     /locus_tag="PLF90_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOK57246.1"
                     /translation="MRILGLDIGTKRIGVAISDESCTIARGLGILERDGKEINKIKEI
                     IEKYNIEKIIYGLPLRMDGSISGQTELVISFISKLKNEIKIPLKEWDERLSTKNAETI
                     LIQADISRKKRKKLIDKLSAQIILQNYLDSKTLENKTIEDIEPEY"
     gene            1079..2095
                     /locus_tag="PLF90_07970"
     CDS             1079..2095
                     /locus_tag="PLF90_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK57247.1"
                     /translation="MRKIKCAVVGTGIFGELHVETYASYDKADLLLICDSNEERAKKV
                     SKKYNVKYTLDYKEVAENEEIEAVSVATPDFAHRDIVVEMLQKGKHVLVEKPMATNVK
                     DAEIMLEATKESGKILMIDFHNRFNPAFIQTKERFDNGDFGDIGMIYTRMSDAISVPL
                     KWFSWSNKSGPQWFLFPHIVDLVTWISGEKVKKVYGIGKKEVLKSHGLDIYDTVTGIL
                     DFGKFYATVETSWIIPEVWTSICDFYFKLEGSKGRVEADFGDMGLKIASDVKKKMEIP
                     LYTGYIPTYGKLQGFQPLPIMYFIDCILENKKPFITPEEDLENVKIISSIIKSVETGK
                     VVEI"
     gene            2107..2883
                     /locus_tag="PLF90_07975"
     CDS             2107..2883
                     /locus_tag="PLF90_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK57248.1"
                     /translation="MKLGIVTYQIAADWDIDTIIEKCKKLGYEGVELRTGHKHGVETT
                     LSKQEREDIKKKFQESNIKLVGLGSAFEYHSPDKEELRKNIEGTKEYIILAKDVEAEG
                     VKVRPNAFPENVPREKTIEQIGMSLREVSSFASDYGIKIRLEVHGPETSHPPYIKQMI
                     DIANHQNLYICWNSNMTDIDETGSIEKNFNLLKDKIDICHINELCNEYPWIELFKLLN
                     RINFNGFCLAEIQASSEPDRLLSYYKALFNAYNKIVQTEK"
     gene            complement(2888..4681)
                     /locus_tag="PLF90_07980"
     CDS             complement(2888..4681)
                     /locus_tag="PLF90_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK57249.1"
                     /translation="MIKEFFRIKNYVKVRWHLLVAGIFFTILSVFANGVSLSAIVPLM
                     DKVLVKKEKIILPENLPQIIKIKIEPLIFKINQLPSLLLLKYLLIFLITVLFLKGLFF
                     YLQNYFYRCFSTRLLTDVRELLYTKIMSLSDDFFAKKQTGELTTRIIYDVNLLNVVFE
                     SFFPKFVFPILLTFSYFVIIFTIDWKMSLLSIFMFPVILYPVFRMGNKLRKLGKKIQI
                     AYGEIGNVINESVYGQQIIKVYNREEYMKEKFKKENENIFKNIISMVKRLLIISPFTE
                     FAGAVASSGLIYYGAVKIINGGMSTGFLFLFFVALLSMISPLKGIGETYGLIKQASSA
                     LPRVFSVLDTESSVVEEGKDVFMGLKRNIEFRDVFFSFEGKQVLKGISFIVEKSEKIG
                     IVGPTGVGKTTLVGLLLRFYNPEKGEILIDGKNIKEYKINSLRQHIGFVSQQPVLFHS
                     TVKENITLGFENEERFKEVIKMDGIAQLINSLPEGENTIVGERGITLSGGQKQLISIA
                     RAIYKDPDILIFDEATSSLDTESEKMIQKAIDQTLENRTSFIIAHRLSTLKKVNKIIV
                     LKDGTIVEEGTHQQLLDKQGVYYTLWQLQFA"
     gene            complement(4678..5775)
                     /gene="lpxB"
                     /locus_tag="PLF90_07985"
     CDS             complement(4678..5775)
                     /gene="lpxB"
                     /locus_tag="PLF90_07985"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOK57250.1"
                     /translation="MKIGIFTGEVSGDNYAAILAKNINEINPDIEIFGTGGKILEKVG
                     VEIIKGMPVGYMGYSSVILKLPSYLKFFKKVIYEIEKRSPDIIVFIDNPAFNLKVAEK
                     LKGKFFNVYYIPPKIWAHNYKRIKKIKKYINFVIPIFPFEENIYKKEGIKCTYFGHPV
                     VDLIKKGEKRNKEKTMIGILPGSRIQEVSYTLPVILDIVYDLKKEIDFDVGISSFDEE
                     IEMVIKKICKRKRIEFEIFKGFPYTLIENSDLILSTSGTVNLEVALLLKPLIVFYKTS
                     FLNYLIGRCMVKLNMVSPVNLFIGENVVPEYIQKIPKEKVKKDIIDLIGKKEIYQKEI
                     KSFKYLKEKIGQKGVSKKVAEFILELTKGKK"
BASE COUNT         2213 a          814 c          861 g         2049 t
ORIGIN      
        1 tttctgcctt agagatggat aggaaattag tagatatatt aaaaacaagt gagtgtgggt
       61 atgttttaat gcatatgaaa ggtaccccaa aaattatgca ggaaaatcca tactataaag
      121 acacaattgg tgaaattaca gaattcttta aaggaaaatt agaatatatt gaaaaaaatg
      181 gaattgatat tgaaagagtt gtaatagacc ctggaattgg atttggaaaa agagttattg
      241 ataatatcct tattctaaaa catcttgata aattcaaaaa atttaagaga cccattttta
      301 ttggaacttc aagaaaagca tttataggga aaattttgaa tttagaagtt gaacaacgac
      361 ttgaaggttc tcttgcaaca gttgtttatg catacttaaa aggagcaaga attttcaggg
      421 ttcatgatgt aaaggaaaca aaaagagttc ttgatattat acaggcaata gaaaatgaga
      481 atattgggac ttgatatagg gacaaaaaga ataggagttg caataagcga tgaaagttgt
      541 actattgcaa gaggacttgg aattcttgaa agagacggaa aagagataaa taaaattaaa
      601 gaaataattg aaaaatataa tattgaaaaa attatttatg gacttccttt aaggatggat
      661 ggttcaataa gtggacaaac agaattagtt atttccttta tttcaaaact aaaaaacgag
      721 ataaaaattc ctttaaaaga atgggatgaa agattgagca caaaaaatgc agaaacaatt
      781 ttaattcaag cagacataag taggaaaaaa agaaaaaaac ttattgataa actttctgct
      841 caaataattt tacagaatta ccttgattca aaaacccttg aaaataaaac aattgaggat
      901 atagaacctg aatattaaat tgcctgataa aatttttcat ggagtcaaaa taaaaagaca
      961 aaaagtaaga ataaaaaagt gttattacta tacttccctc atacacattt tgacttttta
     1021 tgtatgactg gttataattt ttaaaattaa taatttagcc aattaaaaag gagatattat
     1081 gagaaaaatt aaatgcgcag ttgttggaac aggtattttt ggtgaattac atgttgagac
     1141 atatgcctct tacgataagg cagacctttt gcttatttgt gattcaaatg aagaaagagc
     1201 aaaaaaagta agtaagaaat ataatgtaaa atatactctg gactacaaag aagttgcaga
     1261 aaatgaggaa atagaggcag taagtgttgc aactcctgat tttgctcata gagatatagt
     1321 tgttgaaatg ctccaaaagg gtaaacatgt tcttgttgaa aaaccaatgg caacaaatgt
     1381 taaagatgcc gaaataatgc ttgaagccac caaagaaagt ggaaaaattt taatgattga
     1441 tttccataat agatttaatc ctgcgtttat tcagacaaaa gagagatttg ataacgggga
     1501 ctttggagat attggaatga tttatacaag aatgagtgat gcaatatctg tccctctaaa
     1561 atggttttcc tggtcaaaca aaagtggtcc acaatggttt ttattccctc atattgttga
     1621 ccttgtaaca tggataagtg gagaaaaggt aaaaaaggtt tatggaatag gtaaaaaaga
     1681 agttttaaaa tcacatggac ttgatattta tgatacagta acagggattc ttgattttgg
     1741 aaagttttat gcaactgttg agacatcatg gattatacct gaggtatgga catcaatatg
     1801 tgatttttat tttaaattag agggaagtaa aggaagagtt gaagcggatt tcggagatat
     1861 gggattgaaa atagcatctg atgtaaaaaa gaaaatggaa attcctttat atactggata
     1921 cattccaact tatggaaaac tacagggatt tcaaccacta ccaattatgt attttattga
     1981 ttgtatactg gaaaataaga aaccatttat tactcctgaa gaggatttag aaaatgtaaa
     2041 aataatttct tctattataa aatcagttga aacaggaaaa gttgttgaaa tttaagggga
     2101 ataaaaatga aattaggaat agtaacttat cagattgcag ctgattggga cattgataca
     2161 ataattgaaa agtgtaagaa attaggttat gaaggtgtgg aattaagaac cggccataaa
     2221 cacggtgttg aaacaactct ttcaaaacaa gaaagagaag atattaaaaa gaaatttcaa
     2281 gagagcaata ttaaacttgt tgggttggga agtgcttttg aatatcatag tcccgataaa
     2341 gaagaattaa gaaaaaatat tgaaggaaca aaggaatata taattttagc caaagatgta
     2401 gaggcagaag gagtaaaagt aagaccaaat gcttttcctg aaaatgttcc aagagaaaaa
     2461 acaatagagc agattggtat gtctttaaga gaagtatctt cttttgcttc tgattacggg
     2521 ataaagatac ggcttgaagt tcatggtcct gaaacatctc atccacctta tataaaacaa
     2581 atgatagata tagcaaacca tcaaaattta tatatttgct ggaattcaaa tatgacagat
     2641 attgatgaaa caggttcaat tgaaaaaaat tttaacctgc ttaaagataa aattgatata
     2701 tgccatataa atgagttatg taatgaatac ccgtggatag aactttttaa acttttaaat
     2761 cgtataaatt ttaatggatt ttgtcttgca gaaattcagg caagttcaga accagataga
     2821 ttactttctt actataaagc actttttaat gcttataata aaattgttca aactgaaaaa
     2881 taaaaaatta tgcgaattga agttgccata atgtatagta caccccttgc ttatcaagta
     2941 gttgttgatg cgttccttct tcaacaattg ttccatcttt aagaacaatt attttattaa
     3001 ctttttttaa tgttgagagt cggtgagcaa ttataaaaga tgttctattt tcaagtgtct
     3061 ggtcaattgc tttttgtatc attttttctg attctgtgtc aagagaagaa gtcgcctcat
     3121 caaaaattaa aatatcaggg tctttataaa ttgctcttgc tattgaaatt aattgcttct
     3181 gccctcctga taaagttatt cctctttcac caacaatcgt attttctcct tctggcaaag
     3241 aatttattaa ctgtgctatt ccatccattt ttattacttc tttaaacctt tcttcatttt
     3301 caaatccaag tgttatattt tctttaactg ttgaatgaaa taaaactggt tgctgtgaaa
     3361 caaaaccaat atgctgcctt aaagaattta ttttatattc tttaatattt ttcccatcaa
     3421 ttaaaatttc acctttttca gggttataaa atcttaaaag tagccctact aaagttgttt
     3481 ttccaacgcc agttggtcca actattccta ttttttcact tttttcaact ataaaactta
     3541 ttccttttaa gacctgtttc ccttcaaaag aaaaaaacac atctctaaac tcaatatttc
     3601 ttttaagtcc cataaagaca tctttccctt cttcaacaac tgaactttct gtatcaagaa
     3661 cggaaaatac tcttggtaaa gcagaacttg cctgttttat caatccatat gtctctccaa
     3721 tcccttttaa tggacttatc attgaaagaa gagcaacaaa aaacaaaaat aaaaagccag
     3781 tactcattcc cccatttatt attttaactg ctccgtaata aataagccca cttgatgcaa
     3841 ctgctcctgc aaattcagta aatggagata ttataagcaa tctttttacc atacttatta
     3901 tatttttaaa tatattttca ttttccttct taaatttctc tttcatatat tcttctctgt
     3961 tgtacacctt tataatttgc tgcccatata cactttcatt tataacatta ccaatttctc
     4021 catatgctat ctgtattttt ttgcctaatt ttcttaattt atttcccatt ctaaaaacag
     4081 gataaagaat aacagggaac ataaatattg aaagtaggct cattttccaa tcaattgtaa
     4141 aaattattac aaagtaggaa aaagtgagta atattggaaa tacaaattta gggaaaaatg
     4201 attcaaaaac aacatttaga aggttaacat cataaattat tcttgttgtt aattcacccg
     4261 tttgtttttt tgcaaaaaaa tcatctgata aactcataat tttggtgtat agtaattccc
     4321 ttacatcagt taataacctt gttgagaaac atctataaaa ataattctgt aaataaaaaa
     4381 atagcccttt aagaaaaaga acagttatta aaaaaataag aaggtatttc aaaagtaata
     4441 aagatgggag ttggtttatt ttaaaaatca atggttctat tttaattttt ataatttgag
     4501 gtaaattttc tggcagaata attttttctt ttttaacaag gaccttatcc attaaaggta
     4561 ctattgctga aagagaaaca ccattggcaa aaacagaaag aattgtaaaa aatatacctg
     4621 ctacgagtaa gtgccaccta acttttacat aatttttaat tctaaagaat tcttttatca
     4681 ttttttccct tttgtcagtt ctaaaataaa ttctgcaact tttttgctta cacctttctg
     4741 tccaattttt tcttttaaat atttaaaact tttaatttct ttttgatata tttctttttt
     4801 accaattaaa tcaattatat cttttttaac cttttctttt ggtatttttt gtatatactc
     4861 aggaacgaca ttttctccta taaaaagatt aactggactt accatgttta attttaccat
     4921 acatctacca ataagataat ttaaaaaaga tgttttataa aaaacaatta gtggtttcaa
     4981 taaaagagcc acttctaaat taacagtgcc acttgtagat aaaattaaat ctgaattttc
     5041 aattaaagtg tacgggaaac ccttaaaaat ttcaaattca attctttttc ttttacatat
     5101 tttttttata accatttcta tttcctcatc aaaagatgaa ataccaacat caaaatcaat
     5161 ttcttttttc aagtcataaa caatatctaa aattacaggt aatgtataag aaacctcctg
     5221 tattctactt ccaggtaata ttccaatcat tgttttttct ttatttcttt tttctccttt
     5281 tttgataagg tctacaacag gatgcccaaa atatgtacat tttatacctt cttttttata
     5341 tatattctct tcaaagggga atattggaat aacaaaattt atatattttt taattttttt
     5401 tattcttttg taattatgag cccaaatttt gggaggaata taataaacat taaaaaattt
     5461 ccctttaagt ttctctgcaa ctttaagatt aaaagcaggg ttatcaataa aaacaattat
     5521 atctggactt cttttttcaa tttcataaat aactttttta aaaaatttta aatatgaggg
     5581 gagttttaaa ataactgaag aatatcccat atatccaact ggcatgcctt taataatttc
     5641 aacaccaact ttctcaagta tttttccacc tgtcccaaaa atttcaatat caggatttat
     5701 ttcgttgata tttttagcaa gaattgctgc ataattgtcc cctgaaactt ctcctgtaaa
     5761 aatccctatt ttcattttaa aatggatgag aaataccaaa aagataaaga tttgagttta
     5821 ttcctgaatt tggatggtct attcctgcat ttgaaatatg tctatatcta tattcaagaa
     5881 aaatgatatt ttttttaaaa gaaaacttaa ttccaccgca tatctggtca acaaaat
//
LOCUS       DAOSAT010000100         5927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_8522_length_5927_cov_4.221901, whole genome shotgun sequence.
ACCESSION   DAOSAT010000100 DAOSAT010000000
VERSION     DAOSAT010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8522_length_5927_cov_4.221901"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLF90_07990"
     CDS             complement(<1..326)
                     /locus_tag="PLF90_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOK57251.1"
                     /translation="MIKKFLLETWYLLNEMSPYLLFGFFIAGILKIIIPAEKIYKYLS
                     TPNFSSILKATLFGIPLPLCSCGVIPVSTHLDKEGASKGATTSFLISTPTTGIDSILA
                     TYSLLGP"
     gene            complement(323..733)
                     /locus_tag="PLF90_07995"
     CDS             complement(323..733)
                     /locus_tag="PLF90_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOK57252.1"
                     /translation="MKKEKIIKILKEKGIKPSIQRIEILNYLIQHKIHPAVDEIYSAL
                     FSKIPTLSLATVYNTLKLFCEKGLVNEILIEEDEVRYDFIEKSHFHFKCKICKNIYDI
                     YKECPIISLKEVDGHRIEEHHIYLIGICKNCGKK"
     gene            complement(815..1357)
                     /locus_tag="PLF90_08000"
     CDS             complement(815..1357)
                     /locus_tag="PLF90_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOK57253.1"
                     /translation="MGLFINFFLGLSLSMDAFSVSVISGSLIEKKKFLNSLKISLSFG
                     FAQFLMPVLGWFTGMKFLKFISSFDHIVAFSILFLIGLKIIYESRKIERRFDISKILI
                     LFILAIATSIDAFAVGLTFSLLKIKVLTPSLIIGFTTFSVCLSGFFLGNKIKKFFGNK
                     LELIAGIILILIGFKVLITG"
     gene            1399..1896
                     /locus_tag="PLF90_08005"
     CDS             1399..1896
                     /locus_tag="PLF90_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOK57254.1"
                     /translation="MIFILSILFSVYMGWSMGANDAANCVGADIGSGVMRVRDGIIIT
                     IFFSFLGAIILGSNVIKTIGKGVVPLNILPYDVANIIGMVSLFGAGTWLTIATYKKYP
                     ISTSHSIVGAVAGAGLAFSTPIYWGKIWQIVICWILTPLGSAIFAFLCYPLVRFFFSL
                     KFIRK"
     gene            1909..2370
                     /locus_tag="PLF90_08010"
     CDS             1909..2370
                     /locus_tag="PLF90_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOK57255.1"
                     /translation="MVFLIYLSSAYLAFSWGANDVANATGVLIGTGKISPKIAALIGA
                     ISMAIGVSTWGYKVIETVGFNIVRLTPLMTIALEIGSSLNVHLYTHFGIPVSTSHSIV
                     GAIWGIGLYQGMRTINLKLAKEIITTWAITPLISGIITYVMTKILLIFIGG"
     gene            2372..3040
                     /locus_tag="PLF90_08015"
     CDS             2372..3040
                     /locus_tag="PLF90_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HOK57256.1"
                     /translation="MERTKNILFWLGQKEEREILNDELKHVEISLKCVEEMKNAIEFF
                     VKGKKDEKEKHITNVKNYEHQGDEIRKKITLELSKGLLFPPDREDLIILNEGLNDIAD
                     GAKGVARLLEFFDEKISEEMGHLLISDAIISLKGGEKLKDAINSLINNDIQKLLEECA
                     HIETLEEEGDDKRRELIKMLMKENLSAPKIILLYEIIDTLENLTDAIKRAGDLVRILG
                     IKSK"
     gene            3037..3885
                     /locus_tag="PLF90_08020"
     CDS             3037..3885
                     /locus_tag="PLF90_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK57257.1"
                     /translation="MNQFNIETYRKKVFKTISKYKLVEKGDKIFIGLSGGKDSGSACY
                     LISEYVKKNKIDCQITAFYIKLGDFIPEEIIETIKKQAEICGIELKIYNILDFGIDYS
                     KIVKLNRPICSSCGTIKRYLMNKIPREEGANKLCTGHHGDDFIVFFMKNIIGKNIDWI
                     SKFTPFLKGNGKQLSRIRPLFFVGGKDNKNFCEEMKFPYINEDICPHKFLKQKMDKRR
                     EKWYSTIDEISKWQPNFKEYFLEGVIEIAKRLNMEMTEVLICEICGEPTNKNICAFCR
                     LLKLQI"
     gene            3892..4590
                     /locus_tag="PLF90_08025"
     CDS             3892..4590
                     /locus_tag="PLF90_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOK57258.1"
                     /translation="MFKNYSIPFSFIAGIISFFSPCILPLIPIYLSYITGYSIEEFKT
                     EKELSYSKIILPSLCFIIGFTIIFVLLGASSTFLGSLILKKKNILKYMRFIGGALIIL
                     FGLHILGIFKIKKFNQEKKLRPKKINSGCAGAFILGIALASGWTPCVGPILTSILIIA
                     SMEETVKRGIILLFFYSLGLGVPFIVISLLTKKIFLFLNKTHKYFNFVKICTALLLIL
                     LGVLLVLNRFNFTL"
     gene            complement(4580..5773)
                     /locus_tag="PLF90_08030"
     CDS             complement(4580..5773)
                     /locus_tag="PLF90_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOK57259.1"
                     /translation="MGNMLIAQSGGPTVVINQSLVGAIIEGKKHSEIKEIYGSLYGIK
                     GILDGNLVDLRREKLSDLKKVALTPCAALGSVRKKPTIEECEEIFKNMQRYNIRYFFY
                     IGGNDSAETAFILNENAKKVGYEFKCFHIPKTIDNDLKENDHTPGYGSAGKFVAMAIM
                     GDNLDNKSLPGVKIDVIMGRHAGFLTAASSLARIYEDDGPHLIYLPERPFIIEKFLKD
                     VGEIYEKYGRCLVAVSEGISDENGEPIAAKFTKEVDAHGNVQLSGTGALGDLLADIVK
                     KELKISRVRADTFGYLQRSFPGVYSEVDAKEAYKVGELAVKYAIKYESGSVAIKREKG
                     EEYKVYYEPVSLQLVARETRSMPDEFINEDSNWVTEKFIKYAKPLVGKLPEIGRLKNY
                     RISKG"
BASE COUNT         2153 a          815 c          851 g         2108 t
ORIGIN      
        1 ggtcctaaaa gtgaataagt cgcaagaatt gaatcaatac ctgttgttgg agtggaaatt
       61 aaaaaggaag tagttgctcc tttacttgct ccttctttgt caagatgagt tgacacagga
      121 ataactccac aggaacataa aggtaaaggg attccaaata aagttgcttt taaaattgaa
      181 gaaaaatttg gagtagatag atatttataa attttttcag cgggaattat tatcttcaaa
      241 attccagcaa taaaaaaacc aaaaagtaaa tacggactca tttcatttaa aagataccat
      301 gtttcaagta aaaatttttt aatcattttt ttccacagtt tttacaaatc cctattaaat
      361 atatatgatg ttcctctatc ctgtgtccat caacttcttt taaacttata atagggcatt
      421 ccttataaat atcgtaaata tttttacaga ttttgcattt aaaatgaaaa tgtgattttt
      481 ctataaaatc atatctgact tcatcctctt caattaaaat ttcatttact aatccctttt
      541 cacagaaaag ttttaaagta ttataaacag ttgcgagtga aagagttgga attttagaaa
      601 ataaggcaga ataaatttcg tcaaccgcag gatgtatttt atgttgaatt aaataattca
      661 atatttctat tctttggatt gatggtttaa ttcctttttc ttttaagatt ttaataattt
      721 tttctttttt cattttaaag tttttaaatt tgtaataatt ttaattttag aacaattata
      781 aattaaattt ttaattctgt caagtttatt ttttttatcc tgtaataagt actttgaaac
      841 ctattaaaat aagaattatt cccgcaatca attcaagttt attcccaaag aattttttta
      901 ttttattacc aagaaaaaaa ccagaaagac agacagaaaa agttgtaaat ccaattatta
      961 atgaaggagt taaaacttta attttaagaa gagaaaatgt aagacctact gcaaaagcat
     1021 caatactggt tgcaattgct aaaataaata aaattaaaat ttttgatata tcaaatcttc
     1081 tttctatttt tcttgattca tatataattt taaggccgat taaaaataaa atggaaaagg
     1141 caactatatg gtcaaaactt gaaataaact ttaaaaattt cattcctgta aaccatccta
     1201 aaactggcat taaaaactga gcaaaaccaa aagataaaga aatttttaaa ctattcaaaa
     1261 atttcttttt ctcaattaaa cttcctgaaa taacagaaac agaaaaggca tccattgaaa
     1321 gagaaagacc gaggaaaaaa tttataaaaa gtcccatttg tgatagaata ttttaacatt
     1381 aaatttaaag ggattaaaat gatttttata ctttcaattc tattttcagt atatatgggc
     1441 tggtcaatgg gtgcaaatga tgctgctaac tgtgttggtg ctgacattgg ttctggtgta
     1501 atgagggtaa gagatggaat aataattaca attttctttt ctttcctcgg agcaattatt
     1561 ttaggttcaa atgtaataaa aacaattgga aaaggagtgg ttcctttaaa tattcttccc
     1621 tatgatgttg caaatataat aggtatggtt tctctttttg gagcaggaac atggttaaca
     1681 atagcaactt ataaaaaata tcctatttca acctctcatt caatagttgg tgctgttgca
     1741 ggtgctggtc ttgctttttc aactccaatt tactggggaa aaatctggca gatagttata
     1801 tgctggattc taactcctct tggttctgcc atatttgctt ttttatgcta tccattagtg
     1861 agattctttt tttcattaaa gttcataaga aaataagaaa aacaacttat agtattttta
     1921 atttatcttt caagtgctta tcttgctttt tcatggggag caaatgatgt agcaaatgca
     1981 acaggagttc taattggaac aggtaaaatc tctccaaaaa ttgctgcttt aataggtgca
     2041 atatcaatgg caattggtgt ttcaacatgg ggatataaag taatagagac agttggtttt
     2101 aatatagttc gtttaactcc tttaatgaca atagcacttg aaattggttc ttctttaaat
     2161 gttcatcttt atacccattt tggtattcct gtttccacat ctcattcaat agttggtgcg
     2221 atatggggaa ttggactata tcagggaatg agaactataa atctgaaact tgcaaaggaa
     2281 attataacaa catgggctat aactccttta atttcaggta taataactta tgtaatgaca
     2341 aaaattttat taatatttat agggggttaa aatggaaagg acgaaaaata tattattctg
     2401 gcttggccaa aaagaagaaa gagaaatatt gaatgatgaa ttgaagcatg ttgagatatc
     2461 tttaaaatgt gttgaagaaa tgaaaaatgc catagaattt tttgtgaaag ggaaaaaaga
     2521 tgaaaaagaa aaacatatta caaatgttaa aaattatgaa catcaaggag atgaaataag
     2581 gaaaaaaatt actcttgaac tttctaaagg tttacttttt cctcctgata gagaagacct
     2641 aattatttta aatgaaggac ttaatgatat agcagatgga gcaaaaggtg ttgcaagatt
     2701 acttgaattt tttgatgaaa agatatcaga agaaatggga catctattaa tttcagatgc
     2761 tatcatttct ttaaagggag gcgaaaaatt aaaagatgct ataaattcct tgataaataa
     2821 tgatattcaa aaattacttg aagaatgtgc tcatattgaa acacttgaag aagaaggtga
     2881 cgataaaaga agagaactaa taaaaatgct aatgaaagaa aatctgtccg ctcctaaaat
     2941 aattttactt tatgaaatta ttgatacact tgaaaatctc acagatgcaa tcaaaagggc
     3001 tggagacctt gtcagaattc ttggtataaa atcaaaatga atcaatttaa tatagaaact
     3061 taccgaaaaa aagtattcaa aacaatatca aaatataaac ttgtagaaaa aggagataaa
     3121 atttttatag gtctttcagg tggtaaggat agtggctcag catgttattt aatttcagaa
     3181 tatgttaaaa aaaataaaat agactgccag attactgcat tttatattaa acttggtgat
     3241 tttattccag aagaaataat tgaaactata aaaaaacagg cagaaatatg tggaattgaa
     3301 ttaaagattt ataatatttt ggacttcggt atagattatt caaaaattgt aaaattgaat
     3361 agacctatat gtagcagttg tggaacaatt aaaagatact taatgaataa aatacccaga
     3421 gaagaaggtg caaataaatt atgtacagga catcatggag atgattttat agtttttttt
     3481 atgaaaaata taattgggaa aaatattgac tggatttcaa agtttacacc atttcttaaa
     3541 ggaaatggaa aacaactatc aagaatcaga cctttatttt ttgttggagg taaagacaac
     3601 aaaaatttct gtgaagaaat gaaattccca tatataaacg aagatatatg tccacataaa
     3661 tttctaaaac agaaaatgga taaaagaaga gaaaaatggt actccacaat agatgaaata
     3721 tcaaaatggc aaccaaattt taaagaatat tttttagaag gtgttattga aattgcaaaa
     3781 aggttaaata tggaaatgac agaggtactt atctgtgaaa tatgtggaga accaacaaat
     3841 aaaaatattt gtgcattttg tagattattg aagttacaaa tataaataaa aatgtttaaa
     3901 aattattcta taccttttag ttttatagcg ggtattattt cttttttcag tccatgtata
     3961 ttacctctaa ttccaattta tttatcatac ataacaggtt attctattga agaatttaaa
     4021 acagaaaaag aattatctta ttcaaaaatt atattaccaa gtttatgttt tattattggt
     4081 ttcacaataa tttttgtttt actgggtgcc tcatctactt ttttaggaag tttaatatta
     4141 aagaaaaaaa atattctaaa atatatgaga tttatagggg gagctttaat cattttattc
     4201 ggtcttcaca tccttggaat ttttaaaata aaaaaattta atcaggaaaa aaaattaaga
     4261 ccaaaaaaaa ttaattcagg atgtgcaggt gcttttattt taggaattgc tcttgcaagt
     4321 ggctggactc cgtgtgtagg tccaattctt acatctattt taataattgc ttctatggaa
     4381 gaaacagtaa aaagaggtat aattttattg tttttttatt ctcttggact tggagttcct
     4441 tttattgtaa tatctttatt aacaaaaaaa attttcttat tccttaataa aactcataaa
     4501 tattttaatt ttgtaaaaat ttgtaccgct ttattactaa ttcttttagg tgttctactt
     4561 gttttaaata ggtttaattt caccctttag aaatcctata gtttttcaat cttccaatct
     4621 ctggtaattt tccaacgagt ggttttgcat atttgataaa tttctcggtt acccagttag
     4681 aatcttcatt tataaattca tctggcatac ttctcgtttc tctcgcaacc agttgaagag
     4741 atacaggttc ataataaact ttatactctt cacctttttc tctctttatt gctacagacc
     4801 cgctttcata tttaatggca tattttacag caagttctcc tactttatac gcttcctttg
     4861 catcaacttc tgaataaact ccagggaaag acctctgtaa ataaccaaaa gtatctgccc
     4921 ttaccctgct gatttttaat tcttttttga cgatatctgc aagtaaatct ccaagtgctc
     4981 ctgtaccgct caactgtaca tttccatgag catcaacttc ttttgtaaat tttgcggcta
     5041 ttggttcacc attttcatca gaaattcctt ctgaaactgc gacaagacat cttccatatt
     5101 tttcataaat ctcacccaca tctttcaaaa atttctctat aataaaaggt ctttcaggaa
     5161 gataaataag atggggaccg tcatcttcgt atattcttgc aagagaactt gctgcagtta
     5221 aaaaaccggc atgtcttccc ataattacat caatttttac tcctggaagt gatttattat
     5281 caagattatc tcccataata gccatcgcaa caaatttacc tgctgaacca tagccaggtg
     5341 tgtggtcatt ttcttttaaa tcattatcta ttgtctttgg aatatggaaa catttaaact
     5401 cataacctac ttttttagca ttttcattta gtataaatgc agtttcagca gaatcatttc
     5461 caccaatata aaagaaatat ctgatattat atctctgcat atttttaaaa atctcttcac
     5521 attcttctat tgttggtttt tttcttacac tgcctaatgc agcacatgga gtcaaagcaa
     5581 ctttttttaa atctgataat ttttctcttc ttaaatctac taaattccca tcaagaattc
     5641 ccttaattcc atataatgaa ccataaattt cttttatttc gctatgtttt ttaccttcaa
     5701 taatagcacc tactaaactc tgatttataa ctacagttgg tccaccactt tgagcaatta
     5761 acatatttcc cattaatttg cccatataac ctccttgttt taaggtaaat ttaagatgat
     5821 aaaattatac attaaatcaa aaaaaattga aagttttttt tataaatagt aaaatgtttt
     5881 caaatggaag gaaagagatt acttttattt actgaaattt tactgat
//
LOCUS       DAOSAT010000101         5850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_8667_length_5850_cov_4.054875, whole genome shotgun sequence.
ACCESSION   DAOSAT010000101 DAOSAT010000000
VERSION     DAOSAT010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8667_length_5850_cov_4.054875"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLF90_08035"
     CDS             <1..220
                     /locus_tag="PLF90_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOK57260.1"
                     /translation="KLADSNGLKSIAFPAISTGIFGYPLKEAGEISIRAIIDTVPELK
                     NIKKIRFVLFDEISFSIFSEELKKYKNL"
     gene            249..1118
                     /locus_tag="PLF90_08040"
     CDS             249..1118
                     /locus_tag="PLF90_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOK57261.1"
                     /translation="MKEKILLDTDIGSDIDDAICLSYLLSNPFCQILGITTVTGESEK
                     RSMMASYLIEKSEKNIPVYPGCEKPIIGEQKQKICPQFEYLEKISYRKSCEKFAWLNF
                     LRKTIRENPGEINLITIGPLTNIGILFSFDPEIPDLLKGLYIMGGMFFGEKKGEWNII
                     CDPIAAHIVFSSNIKNVYVCGLDVTTKVIMKKNEVIEKFSKKSLLKEVLNFAEIWFRG
                     RDTITFHDPLAATVLFKKDICEFKKGKVNVNIENKEKFGITGFIEDEKGNINVAFNVK
                     PDDFFLHFFSVFS"
     gene            complement(1102..1761)
                     /gene="queC"
                     /locus_tag="PLF90_08045"
     CDS             complement(1102..1761)
                     /gene="queC"
                     /locus_tag="PLF90_08045"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002789281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOK57262.1"
                     /translation="MKKAICLVSGGIDSFVTAAIAKKMGYKVYCLTVDYGQKARKEIE
                     ASKKVAEFLKAEEHKILKIDLSFLKSALTRNEIKIPEKKTKDIPPTYVPARNTIFISL
                     ALGYAENVGADAIFIGVNSVDFSGYPDCRPIYIKRFQKLIDVATKRTLEGKKIKLITP
                     IINFSKKQIIKKAISLGLDLSITWSCYKNEEKPCGKCPSCRIREKALSELFSEKTKKK
                     H"
     gene            complement(1758..2354)
                     /locus_tag="PLF90_08050"
     CDS             complement(1758..2354)
                     /locus_tag="PLF90_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOK57263.1"
                     /translation="MIYRVTEIFYSLQGEGLLQGLPFIFLRFSGCNLKCFFCDTKYAW
                     KKGKKMTEGEILKEIEKFECKRVCFTGGEPFLQEIECILKILKEKKYWISVETNGTIW
                     KDIKFDWITVSPKIEGMKFHKCGYDERFRDTGDEFKYVIVDEKTFDFIDEKIKKPVIL
                     QPVNNDPVISEKIVEFLKSKQKDNWYLRLQIHKILKIK"
     gene            2440..2928
                     /locus_tag="PLF90_08055"
     CDS             2440..2928
                     /locus_tag="PLF90_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOK57264.1"
                     /translation="MRKKGFTLVEVTFVAGIVTSLSLSVYTAAIQRGKATDCLNNLKQ
                     IYQALVMFQQDNDRLPDAKFFPSSINDQKGIHNILKSYNIKGKVLFCPSLPDELNKYG
                     TNYIWNDNVNGRRLEDLSSSTWLLTEMTAVSKDIPPPHTGRYSIIYPDGRVSTEPKIN
                     LK"
     gene            2934..3485
                     /gene="yfcE"
                     /locus_tag="PLF90_08060"
     CDS             2934..3485
                     /gene="yfcE"
                     /locus_tag="PLF90_08060"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006988.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOK57265.1"
                     /translation="MKIGIISDTHGSLGGWEKGWEILKDSDIIIHCGDLFNHGPGNPV
                     PEKYSPKELLNIFNSLKIPLLISKGNCDSEVDQTFLNIPMASPFLFYQINNLRFIVSH
                     GHIFRKEEWTELGKKWKVNFLISGHTHIPSLEKIENIIILNPGSPSIPKEKIPTSAMI
                     DLSEKSVKIYNLFDQSIIKKENL"
     gene            3488..4708
                     /locus_tag="PLF90_08065"
     CDS             3488..4708
                     /locus_tag="PLF90_08065"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOK57266.1"
                     /translation="MRVEVINIGSELLFDRVNTNINLISRILLQSGFKISRCTVVGDN
                     KEEIKKTFLNSFEESDLIIITGGLGPTFDDLTREAIAEALNRKLIFDEKIWKNIEEKF
                     KRRNITPPEINKRQAYIIEGSQIIENENGTAPGMLIEKNNKLILILPGPPNELKPMLE
                     KTVKLIEEKFKKEEVVSKIYSIIGVPESEVETKLSDLINEMKENLTILARPNLIELVL
                     ILSKKEGKKFEKVENIIKERFGVNFLGVNLPSIPELIGNLLKEKKLKLAIAESCSGGL
                     ACKLITDIPGSSEYFMGGFITYSNILKKKILKVPKTILRKYGAVSEQVAIYMAKNAKK
                     YGKADISLSFTGIAGPTGGTLEKPVGLVWIGVAYKKSVFARNFIFSGDRQTIRERAVY
                     KGFELLREILMKNG"
     gene            4701..5696
                     /gene="mltG"
                     /locus_tag="PLF90_08070"
     CDS             4701..5696
                     /gene="mltG"
                     /locus_tag="PLF90_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOK57267.1"
                     /translation="MDKKNRTLLLIFIIIITGFFLIKIITLPPLVPVTKLVEIPENKN
                     AYEVAKILKEEGIIKSTKWFLYWTNKYNVQKKIKSGIYEFSGRTPLKRVIEKLVKGEI
                     ALVKITVPEGSNIYDIGEILEKSHLIKDKNEFIEYAKNNNFEGFLFPDTYYFPYNISI
                     ESICIKMWKNFKNVFEQIYDEEIHEKNWKKVKEIVTVASIVEKEAGLSSERKIIAGII
                     YKRLKKNIPIESCSTVEYALGYKKKRLTKSDLKIKSPYNTYIYKGLPPTPICNPGKDS
                     LIAALNPINTDYMFFLSKGDGTNYFSKTYSEHLEAIKKYLSNKITPETDLQQNSM"
BASE COUNT         2243 a          744 c          882 g         1981 t
ORIGIN      
        1 aaaacttgct gatagtaatg gtcttaaatc aattgctttt cctgcaattt caactggaat
       61 ttttggttat cctttaaaag aagcaggaga aatttcaatc agagcaatta tagatactgt
      121 tcctgaatta aaaaacataa aaaaaataag atttgttctt tttgatgaaa tttcattctc
      181 aattttttca gaagaactca aaaaatataa aaatctttaa tattgtacca atttaataag
      241 gaaatattat gaaagaaaaa attctgcttg atactgatat cgggtctgat attgatgatg
      301 cgatatgtct ctcttattta ctttcaaacc ctttttgtca aattcttgga attacaacag
      361 ttacaggaga atcagaaaaa agaagtatga tggcgagtta tctaatagaa aaaagcgaaa
      421 aaaatattcc tgtttatcct ggatgtgaga aaccaataat aggtgaacaa aaacagaaaa
      481 tatgccctca atttgaatat cttgaaaaaa tttcatatag aaaatcatgt gagaagtttg
      541 cctggttaaa ttttttaagg aaaacaatta gggaaaatcc aggtgaaata aatttaatta
      601 caattggacc attaacaaat ataggaattc ttttcagttt tgaccctgaa attcctgatt
      661 tattaaaagg actctacata atgggaggaa tgttttttgg agaaaaaaag ggagaatgga
      721 atataatctg tgaccctatt gctgcacata tagttttttc aagtaatata aagaatgttt
      781 atgtttgtgg tcttgatgtt accacaaaag ttataatgaa aaaaaatgag gtcattgaga
      841 aattttcaaa aaaaagtttg ttaaaagaag ttttaaattt tgctgaaata tggttcagag
      901 gaagggatac aataactttt catgacccac ttgcagcaac tgttttattt aaaaaggata
      961 tatgtgaatt taaaaaagga aaggtaaatg ttaatataga aaataaggaa aaatttggaa
     1021 taacaggttt tattgaagat gaaaaaggga atattaatgt cgcttttaat gttaaacctg
     1081 atgatttctt tttacatttc ttcagtgttt tttcttagtt ttttctgaaa acaattcaga
     1141 aagcgccttt tctctaattc tacaacttgg acattttcca cagggttttt cttcattttt
     1201 atagcatgac catgttattg ataaatcaag accaagagaa attgcttttt taattatttg
     1261 ttttttagaa aaatttatta ttggggtaat aagttttatt tttttccctt caagtgttct
     1321 ttttgttgct acatctatca gtttctgaaa tctttttata taaatgggtc tgcaatcagg
     1381 ataacctgaa aaatcaactg agttaacacc tataaatatt gcatctgctc caacattttc
     1441 agcataacca agagcaagag atataaatat tgtatttctt gctggaacat aggtaggtgg
     1501 aatatctttt gttttttttt caggtatttt tatttcattt cttgttaaag ccgatttcaa
     1561 aaaagacagg tcaattttta aaattttatg ttcttctgcc tttaaaaatt ctgcaacttt
     1621 ttttgatgct tctatttctt tccttgcctt ctgtccataa tccactgtca gacagtaaac
     1681 tttatatccc attttttttg caattgctgc tgtaacaaaa ctatcaattc cacctgaaac
     1741 aagacatatt gcttttttca ttttattttt agtattttat gtatttgtaa tcttaaatac
     1801 cagttatctt tctgtttgct ttttaaaaat tcaactattt tttctgaaat tacagggtca
     1861 ttattcacag gttgtaaaat tacaggtttt tttatttttt catcaataaa atcaaaagtt
     1921 ttttcatcaa ctatcacata tttaaattca tcacctgtat ctctaaatct ttcatcataa
     1981 ccacacttat gaaatttcat cccttcaatt ttaggactta cagtaatcca gtcaaatttt
     2041 atgtctttcc agattgttcc atttgtttca actgatatcc agtatttttt ttctttcaat
     2101 atttttaaaa tacactcaat ttcctgtaaa aatggttctc cacctgtaaa acagaccctt
     2161 ttacattcaa atttttctat ttcttttaag atttctcctt cagtcatttt ctttcctttt
     2221 ttccatgcat attttgtatc acagaaaaaa catttcagat tacatccgga aaatctcaga
     2281 aaaataaaag gtaaaccctg taacaatccc tctccctgta atgaatagaa aatttctgtt
     2341 actctataaa tcatttttaa aagtttactt cacctacgtt tttatgtcaa aaaacaactt
     2401 gcatttgaca tccacttaat ttaaagttaa aataaaaata tgagaaaaaa agggttcaca
     2461 ttggtagaag taacttttgt agcaggaatt gtaacatctc tttctttgag tgtatatacc
     2521 gcagcaatac agagaggtaa agcaactgat tgtttaaaca atttaaaaca gatatatcag
     2581 gcacttgtta tgttccagca ggacaatgat agattacctg atgcaaaatt tttcccatct
     2641 tcaattaatg atcaaaaagg aatccataat attttaaaat cttataatat aaaaggtaag
     2701 gtattattct gtccttcatt acctgatgaa cttaataaat atggaacaaa ttatatatgg
     2761 aatgataatg taaacggaag aaggttagaa gatttatcct cttcaacatg gcttttaact
     2821 gaaatgacag cagtaagtaa agatattcca cctccccata caggaagata ttcaattatt
     2881 tatccagatg gtcgtgtttc aacagaacct aaaataaatc taaaataaaa aatatgaaaa
     2941 taggaattat aagtgatact cacggtagtt tgggtggttg ggaaaaagga tgggaaattt
     3001 taaaagattc tgatattata attcattgtg gagacctctt taaccatgga ccaggaaatc
     3061 cagttcctga aaagtattct ccaaaggaat tattgaatat atttaacagt ttaaaaattc
     3121 cacttttaat atcaaaagga aactgcgatt cagaagttga ccagacattt ttaaacattc
     3181 caatggcaag tccttttctt ttttatcaaa taaataattt gagatttatt gtatcacatg
     3241 gacatatttt tagaaaagaa gaatggacag aacttggaaa aaaatggaag gttaattttt
     3301 taatttcagg acatactcat ataccttcac ttgaaaaaat agaaaatatt ataattttaa
     3361 atccaggaag tccttctatc ccaaaggaaa aaatacccac ctccgcaatg attgacttaa
     3421 gtgaaaagtc tgttaaaatt tataatcttt ttgaccagtc aataattaag aaagaaaatc
     3481 tataaaaatg agagttgagg ttataaatat tggctctgaa ttactttttg atagagtaaa
     3541 tacaaatata aatttaattt caagaatttt gttgcagtca gggtttaaaa tatcaagatg
     3601 tacagttgtt ggtgataata aagaggaaat aaaaaaaaca tttctaaatt cttttgagga
     3661 atcagacctt ataataatca caggaggact tggaccaact tttgatgatt taacaaggga
     3721 ggcaatagca gaagcattaa atagaaaact tatttttgat gagaaaatct ggaaaaatat
     3781 agaagaaaaa tttaaaagaa gaaatattac accaccagaa ataaataaaa gacaggcata
     3841 tataattgaa ggtagtcaaa taattgaaaa cgaaaatgga actgcacctg gaatgttaat
     3901 tgaaaaaaat aataagttga ttttgatatt accgggacct ccaaatgaac ttaaaccaat
     3961 gcttgaaaaa actgttaaat taattgaaga aaaatttaag aaagaagaag tagtttcaaa
     4021 aatttattct attattggag ttccagaatc agaagttgaa acaaaattaa gtgatttaat
     4081 aaatgaaatg aaagaaaatt taacaattct ggctcgtcca aatttaatag aacttgtttt
     4141 aatattatca aaaaaagagg gaaaaaaatt tgaaaaagtt gaaaatatta taaaagaaag
     4201 gtttggtgtt aattttcttg gagttaatct tccttctatt ccagagttaa tcggaaattt
     4261 attaaaagaa aaaaaattaa aacttgctat tgcagaatca tgttcaggcg gtcttgcatg
     4321 caaattgatt acagatatac ccggtagttc tgaatatttt atgggtggat ttataactta
     4381 cagtaacatt ttaaagaaaa aaattttaaa agtaccaaaa acaattttaa ggaaatatgg
     4441 agcagtaagt gaacaggtcg caatatatat ggcaaaaaat gctaaaaaat atggaaaagc
     4501 agatatttct ttaagtttta ctgggattgc tggaccaacc ggagggactt tagaaaaacc
     4561 agttgggctt gtatggattg gtgttgcata taaaaaaagt gtttttgcaa gaaactttat
     4621 tttttcagga gacagacaaa ctataagaga acgagcggtt tataaaggat ttgaattatt
     4681 gagggaaatt ctgatgaaaa atggataaaa aaaatagaac ccttctttta atattcatta
     4741 ttataataac tggatttttt ttaataaaaa ttataacact acctccttta gttccagtaa
     4801 caaaacttgt tgaaataccg gaaaataaaa atgcttatga agttgctaaa attttgaaag
     4861 aggaaggaat tattaaaagt actaaatggt ttttatactg gacaaataaa tataatgttc
     4921 agaaaaaaat aaaatctgga atatatgagt tttcaggaag gacaccctta aaaagggtta
     4981 ttgaaaaact tgtaaaggga gaaattgcac ttgttaagat tacagttcct gaaggttcaa
     5041 atatttatga tatcggagaa atacttgaaa aatcacatct tataaaagat aaaaatgaat
     5101 ttatagagta tgctaaaaat aataattttg agggttttct tttccccgat acttattatt
     5161 ttccttataa tatttcaatt gaaagtatat gtataaaaat gtggaaaaat tttaaaaatg
     5221 tttttgagca gatttatgat gaagaaatac atgaaaaaaa ctggaaaaaa gtaaaagaaa
     5281 ttgtgactgt cgcttcaata gttgagaaag aagcaggttt aagttcagaa agaaaaataa
     5341 ttgcgggaat tatatataaa cgactcaaaa aaaatatacc gattgaaagt tgttcaactg
     5401 ttgaatatgc tctcggatat aaaaaaaaga ggttaacaaa atcagacctt aaaataaaat
     5461 caccttataa cacctatatt tataaagggc ttccacctac tcctatatgt aatcctggaa
     5521 aggattcttt gattgctgct ttaaatccaa taaatacaga ttatatgttt tttctttcaa
     5581 aaggagatgg aacaaattat ttttcaaaaa catattcaga acatcttgaa gcaattaaaa
     5641 aatatctatc taataaaata acccctgaaa ccgatttaca acagaactca atgtgatata
     5701 aaattttaat gacttattta ggtagtgaaa taaaaaataa tagagcaatt gtcaaaaaga
     5761 ataaaagagg agtataataa aagaaaaggg gaagaaaatg gaaaaggcat taatgttttt
     5821 gagttttttt cttgggtttt tgagtgtagt
//
LOCUS       DAOSAT010000102         5694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_8972_length_5694_cov_5.229651, whole genome shotgun sequence.
ACCESSION   DAOSAT010000102 DAOSAT010000000
VERSION     DAOSAT010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8972_length_5694_cov_5.229651"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..1723)
                     /locus_tag="PLF90_08075"
     CDS             complement(254..1723)
                     /locus_tag="PLF90_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57268.1"
                     /translation="MKILYKNENLRNPEVSIILIDWSVRHSFHILEYLNNQTIDRNNY
                     EIIWIEYYDRKPEKIEKKLNNSIKKGKSVLDKWVILEMPREVYYHKHLMFNVGIILSE
                     GEIICIPDSDAMVKKTFVETIIKEFKKNQNIVLHIDEIRNENKKFYPFNYPSFEEVLG
                     EGRVLRWDGKTDIEKLDDPIHLLNYGACMCAKKEDLIKIGGADEHIDYLGHICGVYEM
                     TFRLRNYGLKEVWLKEEFLYHTWHPGESGSFDYMGPHDGLNMSTTALNVLITKRILPL
                     QENKSIKIIREKKLLFKTEELLKLAIPEKEIIQTWTVDVLREKEIEKILFPLDKKIRI
                     FVIFIKTTFKKENLKKIKEIFEKDCFPFLKKLKSKKSRFLIIKFVKLVLKIFGDIYWN
                     FYYLNKKVKSNINNISLLFYNHIIPEKNKKFFFTGKDDYTFIYIYLAKKFNINFKNLD
                     LNQPLNFDGKFVITSYKNKEKFYKKLKKYGINDNDIILP"
     gene            complement(1732..2979)
                     /locus_tag="PLF90_08080"
     CDS             complement(1732..2979)
                     /locus_tag="PLF90_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK57269.1"
                     /translation="MEIAIKVEGLSKKYRIGERLPYKTLREELIKFFKGEKKKEDEYI
                     WALKNVSFEIKKGEVVGIIGSNGAGKTTLLKILSRVTEPTEGYAEVYGRVGSLLEVGI
                     GFHPELTGRENIYLNGAMLGMSKKEIGRKFDEIISFSEMEKFLDTPVKYYSSGMYVRL
                     AFSVAAHLETDILLVDEVLAVGDISFQKKCLGKMGDITKEGRTVLFVSHNMGAIRSLC
                     EKVIWLEKGKIKKIGNSEKITTEYEEIYTQKYGISSIRERDKEEIKGKKFYFKKVEIF
                     DENGNSRVTFKYNEKIVLVVEPEGEIDEKYHIVFRIYDKHGIMLFSGASAELHNKYFE
                     PDVKKIKIEIGPTNLTTGIYRIQLLLRKGKIQSGARDEIDIWENAMNFEITDCRPFET
                     NWELQNPRDGIFVMNNKIYGIER"
     gene            complement(2982..3800)
                     /locus_tag="PLF90_08085"
     CDS             complement(2982..3800)
                     /locus_tag="PLF90_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK57270.1"
                     /translation="MEKIIIKPSSKWGKLNLKDIFEFRELLFFLVWKDIKVKYKQTIF
                     GIGWAILQPLFMMIIFTLFFGKLAKIPSDNIPYPVFSYTGLIIWSYFSNSLLFATESI
                     VKHQNVITKIYFPKILLPLSSIISHLLDFIISFFVLILFLLFYKINPSINLIYFPLFL
                     LIAVITAFSVSLYLSALNVYYRDVRYIMNFLIQVWFFLSPIAYSSSIVPERYRFLYGL
                     NPMVGVIEGFRWCLLGKEITSIKMMYFSIFIILILFIGGIYFFKNMEKSFADVI"
     gene            complement(3848..4030)
                     /locus_tag="PLF90_08090"
     CDS             complement(3848..4030)
                     /locus_tag="PLF90_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57271.1"
                     /translation="MENKIIKNRMDLEKYREKIFKFKEKCRKEFVNLPFEKKLKLSFQ
                     LAERLKYLKKFKIIKD"
     gene            complement(4020..4499)
                     /locus_tag="PLF90_08095"
     CDS             complement(4020..4499)
                     /locus_tag="PLF90_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57272.1"
                     /translation="MEKKRILKNYAIGGAVATIYYTEPFFTQDIDIFFIPSNVKKLII
                     LTQFYDFLLKKGGKVYKEYILINKIPVQFIPASKDLEKEAIENSVKVKFKNIEIKILK
                     AEYLIAIFLDVFRPKDREKIIKLIEQSKIDKKVLFDILKRYNLYEKFNEFIEKYYGK"
     gene            complement(4534..4998)
                     /locus_tag="PLF90_08100"
     CDS             complement(4534..4998)
                     /locus_tag="PLF90_08100"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOK57273.1"
                     /translation="MDEIKEKFIELAGNIGESLGLNRTTCQIYALLYINSSPLSPGQI
                     TKELGISKGNVSINLKKLEEWNAIKKVWKKGYTRALYKANEDMEEIIYNKLQIGITKR
                     INEIRKYIEEIKNSLNKQKKKETLKKIEQIEGLINKLEFLNKNLKYLKNNER"
     gene            complement(5061..>5694)
                     /locus_tag="PLF90_08105"
     CDS             complement(5061..>5694)
                     /locus_tag="PLF90_08105"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOK57274.1"
                     /translation="KYIDKLDESAEILGVVNTIHNINGKLKGYTTDGDGFIRSLKEDG
                     KFNPECKTVLIIGAGGSSYAISGALIKEKVNFIYITNRTYENAKKLKEHIVQKLNFKN
                     IEIIDFEKRNEYKIWNNVDLLVNTTSVGMKENDPLLIKKENIEKVKFVYDIVYNRKTE
                     LLKEAEKLKIPYLDGLSMLVYQGAISFEIWTGKKAPTEIMKKSLYKKFKT"
BASE COUNT         1850 a          880 c          541 g         2423 t
ORIGIN      
        1 ataatcaggt ttgaaatttt caacaatttt aacaacctct tcattcattt tttttattcc
       61 tatttctgca attctttttt gaaaatcata ggtaaaaaca gattaaaaaa ttttttttaa
      121 aggttctaca aaatataaat caaaagggca tgtcaatttt aaataagaag aagcatattt
      181 atagattttg taagaagtta agtcatataa aggtgtaaga attaaaactt ttttctggta
      241 ttttgtattc attttaaggt aaaattatat cattatcatt tattccatac ttttttaatt
      301 ttttataaaa tttttcttta tttttataag aggtaattac aaattttcca tcaaaattta
      361 atggctggtt caaatctaaa tttttgaaat taatattaaa ttttttagcc aaatagatat
      421 aaataaaagt ataatcgtct ttcccagtaa aaaaaaactt tttatttttt tcaggaatta
      481 tatggttata aaaaagcagg gaaatgttat tgatattgct tttaaccttt ttattgagat
      541 aataaaaatt ccaataaata tctccaaaaa tttttaaaac caatttcaca aatttaatta
      601 ttaaaaatct acttttttta gattttaatt ttttaagaaa tggaaagcaa tctttttcaa
      661 aaatttcttt aatttttttc aaattttctt ttttaaaagt tgtttttata aaaattacaa
      721 aaattcttat ttttttatcc agtggaaaaa gtatcttttc tatttctttt tcccttaaca
      781 catcaacagt ccaggtttga ataatttctt tttcaggtat tgctaatttt aaaagttctt
      841 ctgttttaaa aagtaatttt ttttctctta taatttttat acttttattt tcctgtaagg
      901 gtaaaattct ttttgttatt aaaacattta aagcagttgt tgacatgttt aaaccatcat
      961 gtggacccat ataatcaaat gaaccacttt ctccaggatg ccatgtatga tataaaaatt
     1021 cttcttttaa ccaaacttct ttcaagccat aatttcttaa tctaaaagtc atttcataaa
     1081 caccgcatat atgacctaaa taatcaatat gttcatctgc tccacctatt tttattaaat
     1141 cctctttttt tgcacacata cacgcaccat aattaagtaa gtgaattggg tcatctaatt
     1201 tttctatatc tgttttccca tcccatctta aaactcttcc ttcacccaaa acttcttcaa
     1261 aagaaggata attaaaggga taaaatttct tattttcatt tcttatctca tctatatgca
     1321 aaacaatatt ttgatttttt ttgaattctt ttattatcgt ttcaacaaat gtttttttta
     1381 ccattgcgtc actatctgga atacaaataa tttctccttc acttaaaata attcctacat
     1441 taaacataag atgcttatgg taataaactt ctctgggcat ttcaagtatt acccatttgt
     1501 caagaacaga ttttcctttt ttaatagaat tattcagttt tttttcaatt ttttccggtt
     1561 ttctatcata atattcaatc catataattt cataattatt tctatcaatt gtttgattat
     1621 tcaaatactc cagaatatga aaactatgcc ttacagacca atcaatcaaa ataatactca
     1681 cttctggatt tctcaaattt tcatttttat aaagtatttt catttttaat tttatctctc
     1741 aatcccataa atcttattat tcattacaaa aataccatct cttggattct gcaattccca
     1801 atttgtttca aatggcctgc agtcagttat ctcaaagttc attgcatttt cccatatatc
     1861 aatctcatct cttgctccac tttgtatctt tccttttctt aataaaagtt gtattctata
     1921 aattcctgtt gttaaattgg taggtcctat ttctattttt atttttttta catctggttc
     1981 aaaatattta ttatgtagtt cagcagaagc acccgaaaat aacataattc catgtttatc
     2041 ataaattcta aagacaatat gatatttttc gtctatttct ccttcaggtt caacaactaa
     2101 aactattttt tcattatatt taaaagtaac tcttgaattt ccattttcat caaaaatttc
     2161 tactttttta aaatagaatt ttttaccctt tatttcctcc ttatctcttt cccttatact
     2221 tgaaattcca tatttttgag tgtatatttc ttcatattca gttgttattt tttcactatt
     2281 tcctattttt tttatttttc ctttttccag ccatataact ttttcacata aacttcttat
     2341 tgctcccata ttatgactta caaataaaac tgttcttcct tcttttgtta tatcacccat
     2401 tttaccaaga cattttttct ggaaggatat atctccaact gcaagaactt catcaacaag
     2461 taaaatgtct gtttcaagat gggctgcaac tgaaaatgca agacgaacat acatccctga
     2521 actataatat ttaacaggtg tatcaagaaa tttttccatt tcagaaaaag atataatttc
     2581 atcaaatttt ctaccaattt cctttttact cataccaagc attgcaccgt ttaaataaat
     2641 attttccctt ccagttaatt caggatgaaa accaatccca acttcaagta aactccctac
     2701 tcttccatat acttccgcat atccttctgt tggttcagtc actcttgata aaattttaag
     2761 taatgttgtc tttcctgctc cgttagatcc aattattcca acaacttctc ctttttttat
     2821 ttcaaatgat acattcttta aagcccatat atattcgtct tctttctttt tctctccttt
     2881 aaaaaattta attaattctt ccctcaatgt tttatacggt aatctttctc ctatcctgta
     2941 ttttttactt aatccttcta cttttattgc tatttccatt tttatattac atctgcaaat
     3001 gatttttcca tatttttaaa gaagtaaata ccgcctataa aaagaatcag aataataaag
     3061 atagaaaaat acatcatttt tattgaagtt atttcttttc caagaagaca ccatctgaaa
     3121 ccttctatta ctccaaccat tggatttaaa ccatataaaa acctgtatct ttcaggaact
     3181 attgaagatg agtaagcaat tggacttaaa aaaaaccaga cctgaatcaa aaagttcata
     3241 atgtatctaa catctctgta ataaacattt aaagcagaaa gatatagaga aacagaaaaa
     3301 gcagttatca ccgctattaa aaggaataaa gggaaataga ttaaatttat tgatggattg
     3361 attttataaa aaaggagaaa tagaattaag acgaaaaaag aaattataaa atcaagaaga
     3421 tgacttataa tggaagaaag aggtaataaa atttttggga aatatatttt tgttataaca
     3481 ttttgatgtt ttacaatact ttctgttgca aaaagtaaac tgtttgaaaa ataactccat
     3541 atgattaaac ccgtatatga aaaaacagga taaggaatgt tatcagaagg tattttagca
     3601 agttttccaa aaaataatgt gaagataatc atcataaata gtggttgtaa aatagcccat
     3661 ccaattccaa aaattgtctg tttatattta acttttatat ctttccagac aagaaaaaaa
     3721 agcagttctc tgaattcaaa tatatctttt aaattcagtt ttccccattt tgaactcggt
     3781 tttattatta ttttttccat tttgtttaat ttattttaaa caaattaaac aattttgtca
     3841 agaaatttta atcttttata attttaaact tttttaaata tttcaatctt tctgccagtt
     3901 ggaaggagag ttttaatttt ttttcaaatg gaagattaac aaattctttt ctacattttt
     3961 ctttaaattt gaatattttt tctctgtatt tttctaaatc cattctgttt tttataatct
     4021 tattttccat agtatttttc tataaactca ttaaattttt catataaatt atatcttttc
     4081 agaatatcaa aaagaacttt tttatctatc ttggattgct caatcaattt tataattttt
     4141 tctctatctt ttggtctaaa aacatccaaa aatattgcta tcaagtattc cgcctttaaa
     4201 attttaattt ctatattctt aaatttaact tttactgaat tttctatcgc ttctttttct
     4261 aaatctttac ttgctggaat aaattgaaca ggaattttat ttattaaaat atattcttta
     4321 taaactttgc cacccttttt taacaaaaag tcataaaatt gggtaaggat aataagtttt
     4381 tttacatttg atgggataaa aaaaatgtct atatcttgtg taaaaaaagg ttctgtataa
     4441 taaattgtgg cgactgctcc tccaatagca taatttttta aaatcctttt tttttccatc
     4501 tgatttatta acttgaaaat tttttctaaa tctttatctc tcattgtttt taagatactt
     4561 taagttcttg tttaaaaatt caagtttatt tattaatcct tcaatttgtt caattttttt
     4621 tagagtttct ttcttttttt gtttatttaa cgaatttttt atttcttcaa tatattttct
     4681 tatttcattt attctttttg taattccaat ttggagttta ttataaataa tctcttccat
     4741 atcttcattc gctttataaa gggctcttgt atatcctttt ttccagacct tttttattgc
     4801 attccattct tcaagttttt ttaaatttat actgacatta cctttactta ttccgagttc
     4861 ttttgttatc tgtcctggag aaagtggaga agaatttata taaagtaaag cgtaaatctg
     4921 acatgttgtt ctgtttaaac ccagactttc tccaatattt cctgctaatt ctatgaattt
     4981 ttcttttatt tcatccattt tcgtttaatt tgttttatat attttaaact ttttacattt
     5041 tattccgatt tttttgtaag tcaagttttg aatttcttgt ataatgactt tttcataatt
     5101 tccgtaggtg cttttttacc tgtccatatt tcaaaagata ttgctccctg atatacaagc
     5161 attgataaac cgtcaagata aggaattttt aatttttctg cttctttcaa taattctgtt
     5221 tttctgttat aaacaatgtc ataaacaaat ttgacttttt caatattttc tttttttatc
     5281 agaagtgggt cattttcttt catgccgaca gaagttgtat taaccagaag gtcaacatta
     5341 ttccatattt tatattcatt tcttttctca aaatcaatta tttcaatatt tttaaaattc
     5401 aatttttgaa ctatatgttc ttttaatttt tttgcatttt cataagtcct gtttgtaata
     5461 taaatgaaat taactttttc tttaattaga gcacctgata tagcataaga tgaaccacct
     5521 gctccaataa ttaaaacagt tttacactcg ggattaaatt ttccatcttc ttttaatgac
     5581 cttataaatc catctccatc ggttgtgtaa ccctttaatt ttccattaat gttatgaata
     5641 gtatttacaa ctccaagaat ttctgctgac tcatcaagtt tatcaatata tttt
//
LOCUS       DAOSAT010000103         5603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_9131_length_5603_cov_6.095350, whole genome shotgun sequence.
ACCESSION   DAOSAT010000103 DAOSAT010000000
VERSION     DAOSAT010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9131_length_5603_cov_6.095350"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLF90_08110"
     CDS             <1..677
                     /locus_tag="PLF90_08110"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK57275.1"
                     /translation="LAVFFYGCSFDCLFCQNYSHKYIEKGKILSIENLLKWAIDDRVY
                     CICFFGGSPEPQFPFIINFCEELMKKRKIRICFEWNGSGNEDFVRKIGEIALKSKGII
                     KFDLKAFDENLNIVLTGVSNKKTYQNFKLIAKEFLKKADYPLLTATTLLVPYYIDEIE
                     VEKISKFISDLSHEIPYSLLIFYPEFYLKDLPVTPKETLYKCYNIAKKYLKKLHIGNL
                     HLLNLI"
     gene            730..1485
                     /locus_tag="PLF90_08115"
     CDS             730..1485
                     /locus_tag="PLF90_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOK57276.1"
                     /translation="MKVKVVEKIMKIIEVIASGKNEGYSVGEISKILNLKITTVHSLL
                     STLLSLGYLCKDEKTKKYKISDKLLNIFSPLINKNILLKVAEPVMKSLAETIKESVVL
                     GIFYKGERYTIAQANYEGHILNVNLNMFQKFSCYLTATGRILLSYLPEEEVKEYIRKN
                     GYPKENWKNIKNYEELKKEFEKIRKNKIEIIEKENTIAIGVPIFNNKKEIVASLGVYL
                     PKIRFKGENKEKITNELKKAGEEITIKLGGKYE"
     gene            1478..3706
                     /locus_tag="PLF90_08120"
     CDS             1478..3706
                     /locus_tag="PLF90_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57277.1"
                     /translation="MNKKLTEEVKKYAKKIGADIVGIADVERFKNAPERMSPKGLLPE
                     SKSVIVCGIHHLDASVELGGEPTPHNMGPYGSQSSVMNPKLDDISFSIARFLEDKGYK
                     TLPIVSSNIWRYKGYKDLKVHFAPDIAHRYAAVAAGLGEIGWSGLFLHPEFGPRVRLV
                     TIITEAELEPDPMYEGEKLCDKCMECVKNCPVDAFRKEVKKINEIEIGGKFFKFPDTN
                     KWRCGWAENFGLNLALKIPEKVEEDVILENLEKYGSFAGEEGSCLKFCMIPQKRYYDK
                     NYCRAPRRKKEKKEIRIEEIKKILKKYKIDYFCIKEKNFFKNDFSINPSLHLPDVSSI
                     ISIGIKKPADTNTEIENYIKWRLIQYTAFEIAHYFDLNGYSGITGTKIDDNLVAQKTG
                     IWEEDTFYTTVLVSAKLPEIKIIRKPEKEKIEKERFRKFCFDNGADLAGFFNLKRFEE
                     FKNEFLKKIKLPEEIERVYDISGIYAPCSPSIKKEKIKLKEPKDYLEDAKSVIVIGLH
                     YPDACIDTAKITPAETVGPYAFSQFETLNLLTDIAYRIIKKLNDCGYKGVYSYDLTGF
                     ASCVLSSRGLLPDNRANSYSVLLSGLGYIGKNGVPITEKFGQRHRFLSIITDFEFEND
                     PLYDGENFCKNCNECVKNCVTEAIKDNVKKIKIENKFFEIPEIDYFACDWAKRYGLIG
                     KEGPEFMGIDINFKVPEKNRIEKLIDNLMKVKWGVQKRHLNICEECIRTCTFKGERRG
                     KW"
     gene            3700..5205
                     /locus_tag="PLF90_08125"
     CDS             3700..5205
                     /locus_tag="PLF90_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK57278.1"
                     /translation="MVKVERGEVKFNERVDVKYEVDVLVVGGGSAGFGAAISSARNGA
                     KTILVEKYGALGGLVTLGLVCYMAGYPEGVGKELIERLREKGGIGEKIRICDPEITKY
                     VMEKMAIESGVKILYWTYVIDSIVKDNEIKGVIIQNISGRSAILAKRVIDCTGDAEVS
                     YFAGVPVEVGWDLMEGYNQAVSLDFVMSNVDLNSFSSSDFYSKVQMKIEEAAKEGKLP
                     RLVEKGYLGPFPNRQADKGDVYVCTAHSRRCKTTDAEDLTRIVIEQRDQIQKLIEFYK
                     ENVVGFEKAYLSYTASILGVRESRRIVGEYKLTGEDLVLARKFKDAIARDTHGFDIHN
                     PIDDLPHIKHTHLSEPKEPAYCTDKEREDGRICCVKNNPKGKYRAYLKPGEYYEIPYR
                     CLIPVKIDNLLVAGRCISTDFEAQSGTRLIMTCITMGHAAGTAASLSIKENITPRKLD
                     VDYLRDKLVKEGINLKEKPPCYVKGGPYEVPKDAEFIVSTKDGKDEIKVKK"
     gene            5222..>5603
                     /locus_tag="PLF90_08130"
     CDS             5222..>5603
                     /locus_tag="PLF90_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57279.1"
                     /translation="MLTSKKVKEVAKKLGADLVGIADINRFEGAPKQMDPRYINPDAK
                     VLIVLALRIPRGALRGIEEGTYFLSYSSQAYGSINMIYMPFILYHLTRFIEDNGYEAT
                     PIPNDYYNWPAIEYHNGKIKENWSR"
BASE COUNT         2180 a          633 c         1076 g         1714 t
ORIGIN      
        1 atcttgctgt atttttttat ggttgttcat ttgactgcct tttttgccag aattattccc
       61 ataaatatat tgagaaagga aaaattttat ctattgaaaa tttactgaaa tgggctattg
      121 atgatagagt ttactgtatt tgtttttttg gaggttctcc agaaccgcaa ttccctttta
      181 tcattaattt ctgtgaagaa ttaatgaaaa aaagaaaaat aaggatctgt tttgaatgga
      241 atggtagcgg aaatgaagat tttgtaagaa aaataggaga aattgctctt aaaagtaaag
      301 gaataattaa atttgattta aaagcatttg atgaaaattt gaatattgtt ttaacgggtg
      361 tttcaaataa aaaaacatac cagaatttta aattgattgc taaagaattt ttaaaaaaag
      421 cggattatcc attactgaca gcaacaactc ttcttgttcc ttattatatt gatgaaattg
      481 aagttgaaaa aatatcaaag ttcatttcag atttaagtca tgaaattccc tattcacttt
      541 taatttttta tcctgaattt tatttaaaag atttacctgt aactccaaaa gaaactcttt
      601 ataaatgtta taatattgca aaaaaatatc tcaaaaaatt acatattgga aatctgcatc
      661 ttttaaactt aatttgaaaa aaaatttttt tatgatatta ttgaataaat acgatattat
      721 aggaataaaa tgaaagtaaa ggtagttgaa aagattatga aaattattga ggttattgca
      781 agtggaaaaa atgagggcta ttctgttggt gaaatttcca aaattttgaa tttaaagatt
      841 acaacagtcc actctctttt atccactctt ctttccctcg gttatttatg taaggatgag
      901 aaaacgaaaa aatataaaat ttctgacaaa cttctaaata tcttttcacc ccttattaat
      961 aaaaatattc ttcttaaagt tgcagaacct gttatgaaat ctcttgcaga aacaattaaa
     1021 gaatcagttg ttcttggtat tttttataaa ggagaaagat atacaattgc acaggcaaat
     1081 tatgaaggac atattttgaa tgtaaatctg aatatgtttc aaaaattctc ttgttattta
     1141 acagcaacag gaagaatttt actttcttac cttcctgaag aggaagttaa ggaatatatc
     1201 agaaaaaatg gttatccaaa agaaaattgg aaaaatatta aaaattatga agaattgaaa
     1261 aaggaatttg agaaaataag gaaaaataaa attgaaatta ttgaaaaaga aaacacaatt
     1321 gcaattggag ttcctatttt taacaacaaa aaagaaatag ttgcttctct tggagtttat
     1381 cttccaaaaa taaggtttaa aggagaaaat aaggaaaaga taacaaatga acttaaaaaa
     1441 gcaggtgaag aaataactat taaacttgga ggtaaatatg aataaaaaat taacagaaga
     1501 ggttaaaaaa tatgcaaaaa aaattggagc agatattgtt ggaatagcag atgttgaaag
     1561 atttaaaaat gcaccagaaa gaatgagtcc aaaaggtctt cttcctgaaa gtaaaagtgt
     1621 tattgtctgt ggaattcatc atcttgacgc atctgttgaa cttggtggag aaccaacccc
     1681 tcataatatg ggaccttatg gaagtcaaag ttctgttatg aatccaaaac ttgatgatat
     1741 ttctttttca atagcaagat ttcttgaaga caaaggatat aagacacttc caatagtatc
     1801 aagcaatata tggaggtata aaggttataa ggatttaaag gtccattttg cacctgatat
     1861 tgcccataga tacgcagcag ttgctgcagg acttggcgag attggttgga gtggtctatt
     1921 tttacatcct gaatttggtc caagggtccg tcttgtaaca attattactg aggcagagtt
     1981 agaaccagac cctatgtatg aaggagaaaa attatgtgat aaatgtatgg aatgtgttaa
     2041 aaattgtcct gtagatgctt tcagaaaaga agtaaaaaaa ataaatgaga tagaaattgg
     2101 aggtaagttt tttaaatttc ctgatacaaa taaatggaga tgtggatggg ctgaaaattt
     2161 tggtttaaat cttgccttga aaatacctga aaaagttgag gaggatgtaa ttcttgaaaa
     2221 ccttgaaaaa tatggttctt ttgcaggaga ggaaggaagc tgtttaaagt tctgtatgat
     2281 accacaaaaa agatattatg ataaaaatta ttgtagggct ccgagaagga aaaaagaaaa
     2341 aaaagagatt agaattgaag aaattaaaaa aatacttaaa aaatacaaaa tagattattt
     2401 ctgtataaag gaaaagaatt ttttcaaaaa tgatttttct ataaatccat ctcttcatct
     2461 tcctgatgtt tcttctatta tctctatagg aattaaaaaa cctgctgata caaatacaga
     2521 gattgaaaac tatataaaat ggagattgat tcagtatact gcttttgaaa ttgctcatta
     2581 ctttgattta aatggttatt caggaattac aggaacaaag atagatgata atcttgtcgc
     2641 acagaaaact ggaatttggg aggaagatac attttataca acagttcttg ttagtgctaa
     2701 attgcctgaa attaaaatta taagaaagcc ggaaaaagag aaaatagaaa aagaaagatt
     2761 tagaaaattc tgttttgata atggtgcaga ccttgcagga tttttcaatc ttaaaagatt
     2821 tgaagaattt aaaaatgaat ttttaaagaa aattaaatta cctgaagaga tagaaagggt
     2881 ttatgatatt tcaggtattt atgctccatg cagtccttca ataaaaaaag agaaaattaa
     2941 attaaaagaa ccaaaagatt atcttgaaga tgcaaaatca gttattgtta ttggacttca
     3001 ttaccctgat gcctgtattg ataccgcaaa aataacacca gcagaaacag ttggacctta
     3061 tgcttttagt caatttgaaa cattgaattt attaacagat attgcatata gaattataaa
     3121 aaaacttaat gactgcggat ataaaggtgt ttattcctat gacttaacag ggtttgcatc
     3181 atgtgtttta agttcaaggg gacttttacc tgataataga gcaaattcat attctgtttt
     3241 actttcaggt cttggttata taggcaaaaa tggagtccca attactgaaa aatttggtca
     3301 aagacataga tttttaagta taataactga ttttgaattt gaaaatgacc ctttatatga
     3361 tggagaaaat ttctgtaaaa attgtaatga atgcgttaaa aattgtgtta cagaagcaat
     3421 aaaagataat gttaagaaaa taaagattga aaataaattt tttgaaatac ctgaaattga
     3481 ctattttgca tgtgactggg ctaaaagata tggacttata ggaaaagaag gacctgaatt
     3541 tatgggaatt gatattaatt ttaaagttcc tgaaaaaaat agaattgaaa aactcattga
     3601 taatttgatg aaggtgaaat ggggagtcca aaaaaggcat ttaaatatat gtgaagaatg
     3661 tataagaacc tgtacgttta aaggggaaag gaggggaaaa tggtaaaggt tgaaagagga
     3721 gaagttaaat ttaatgaaag agtagatgtt aagtatgaag ttgatgttct tgtagttgga
     3781 ggtggttctg ctggatttgg tgctgctata agcagtgcaa ggaatggagc aaaaacaata
     3841 cttgttgaaa aatatggtgc tcttggaggt cttgtaactc tgggacttgt ctgttatatg
     3901 gcaggctatc ctgaaggagt tggaaaggaa ttgatagaaa gattgagaga aaaagggggg
     3961 attggagaaa agataaggat atgcgaccct gaaataacaa aatatgtaat ggaaaaaatg
     4021 gcgatagaaa gtggagttaa aattttatac tggacatatg ttattgattc aattgttaag
     4081 gataatgaaa taaaaggagt tataattcag aatatttcag gaaggagtgc aattttagca
     4141 aagagggtga ttgactgtac aggagatgcc gaagtttctt attttgcagg agtacctgtt
     4201 gaagttggat gggatttaat ggaaggatat aatcaggcgg tctctcttga ttttgttatg
     4261 tcaaatgtag atttaaattc attttcttca tctgatttct attcaaaagt ccagatgaaa
     4321 atagaagaag cagcaaaaga agggaaactg ccacgactgg ttgagaaagg ttatttaggt
     4381 ccttttccaa atagacaagc agataaagga gatgtatatg tctgtacagc acacagcaga
     4441 agatgtaaaa caacagatgc agaggattta acaagaatag taattgaaca gagagaccag
     4501 atacagaaac taattgagtt ttataaggaa aatgttgttg gatttgaaaa ggcatacctt
     4561 tcttatacag catcaatttt aggagtgaga gagtcaagga gaattgtagg tgagtataaa
     4621 ctgacaggtg aggaccttgt tcttgcaaga aaatttaagg atgcaatagc gagagataca
     4681 catggttttg atatacataa tccaatagat gacctacccc atataaagca tacacatcta
     4741 tctgagccaa aagaacctgc atattgtaca gacaaagaaa gagaagatgg aagaatttgt
     4801 tgtgttaaga ataatccaaa aggtaaatac agagcatatt taaaaccggg tgagtattat
     4861 gaaattccgt atagatgtct tatcccggtt aaaatagata atctacttgt tgcaggaaga
     4921 tgcatttcaa ctgattttga agcacaatca ggaacaagat tgattatgac atgcataact
     4981 atgggacatg ctgctggaac tgctgcatct ctttcaataa aggaaaatat aactccaaga
     5041 aaacttgatg ttgattattt aagagataaa cttgtaaagg aagggataaa tttaaaagaa
     5101 aagccaccat gttatgttaa aggtggtcct tatgaagttc caaaagatgc tgaatttata
     5161 gttagtacaa aagatggtaa agatgaaata aaagtaaaaa aataaaagag agggagggaa
     5221 aatgttaaca tcaaaaaaag tgaaagaggt ggcaaaaaaa ttaggagcag atttagtggg
     5281 gattgctgat ataaacagat ttgaaggagc accaaaacaa atggacccaa gatatataaa
     5341 tccagatgca aaggtattga tagttcttgc tttgagaatt ccaagaggag cattaagagg
     5401 tatagaagaa gggacctatt ttctttcata ttcctctcag gcgtacggaa gtataaatat
     5461 gatttacatg ccttttattt tgtatcacct tacaagattt attgaggaca atggatatga
     5521 agcaactcct ataccaaatg attattataa ctggccagca attgaatatc acaatggaaa
     5581 aataaaagaa aactggtccc gtc
//
LOCUS       DAOSAT010000104         5585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_9169_length_5585_cov_5.090054, whole genome shotgun sequence.
ACCESSION   DAOSAT010000104 DAOSAT010000000
VERSION     DAOSAT010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9169_length_5585_cov_5.090054"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PLF90_08135"
     CDS             complement(<1..548)
                     /locus_tag="PLF90_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK57280.1"
                     /translation="MEIKISFIGCGGIANAHMNALSKIEGVKFVGMCDVDENKAKAAS
                     EKFGGKVYTDYKKMLEQLELDACYICVPPFAHEEQEEMCIEKNIPFLVEKPIHLNKNK
                     VKKIVEKIKEKNLITAVGYQDRYQDIIAYIKPFFSEGNLGFFAGWWVGGMPGVYWWRR
                     KEMSGGQAVEQTTHIFDMARYLV"
     gene            complement(565..1368)
                     /locus_tag="PLF90_08140"
     CDS             complement(565..1368)
                     /locus_tag="PLF90_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK57281.1"
                     /translation="MKIGCQEGLLPGRDLSEKFEIARKIGFEGIEVWGRGLLEVPEKI
                     DEYKKISEKIGIKISSICAGYRNFLLEAEKENRTKAREDIKKLLEIGKELEVIGLILV
                     PIFGSPKVPDLTPYKNAYELEKELLFSQLPEIVEKAEETKCALLLEPLNRYETHFLNR
                     LEQAVEYCEKINSPYLKIMADFFHMSIEEADIAESIERAGKWIAHVHLADSNRILPGM
                     GHTDFESGFKALKKINFENYMVLECGVPEPRKENLRKSFNFMKNILNKI"
     gene            1480..2310
                     /locus_tag="PLF90_08145"
     CDS             1480..2310
                     /locus_tag="PLF90_08145"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOK57282.1"
                     /translation="MRVGAHLWIGEGIEKTLEYCEYLGCNCFQIFLSNPRSWKRKEKS
                     IEEIIRFREKIKLKKIKPIVIHMPYILNVAENNIKEKKKIVQLLEKEIEESENLKADF
                     YVLHPGFHKGIGEKKGIENVINTLKNFSISKIKILLENTSGQGTSIGYKFEHFSFLFD
                     RLGDNFGVCFDTAHAFQSGYNLKNFEKVKENIEKFIPLSYILLIHANDSKTELNSKTD
                     RHEHIGKGKIGIKGFEEIIKDEYFGNLSYIIETPKLSLKEDKKNIEILKKIGEKYGKI
                     "
     gene            2297..4744
                     /gene="leuS"
                     /locus_tag="PLF90_08150"
                     /pseudo
     CDS             2297..4744
                     /gene="leuS"
                     /locus_tag="PLF90_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
     gene            complement(4741..4983)
                     /locus_tag="PLF90_08155"
     CDS             complement(4741..4983)
                     /locus_tag="PLF90_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57283.1"
                     /translation="MTAIIGGIIAIAIGLWLIFPGPLGQATKWFIGGGLVVMLILGGI
                     IAVAAGISDIKDRIEEKREKEKEEEKKESEIEKKEG"
     gene            complement(5040..>5585)
                     /locus_tag="PLF90_08160"
     CDS             complement(5040..>5585)
                     /locus_tag="PLF90_08160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK57284.1"
                     /translation="HLYKILEGIKKTGKNLRFHTPNGIHPKFIEKEVAYILKDVNFKT
                     IRLSLETIEEKRREESGYKVNFSEFEKCIKNLVDAGFTEKEIGVYILAGLPGQTPKEV
                     INTIKVLKNYPVKIKMAEYSPIPETVDFEIAKNLYPHLPLSDPLFQNNSIYPLWNFEN
                     KWEIINQIKQMIKDTKIDKFL"
BASE COUNT         2047 a          800 c          829 g         1909 t
ORIGIN      
        1 acaagatatc ttgccatatc aaaaatatgt gttgtttgtt caactgcctg tcctcctgac
       61 atttcttttc ttctccacca gtaaacacca ggcattcctc caacccacca gccagcaaaa
      121 aatccaagat taccttcaga aaaaaatggc ttaatatagg caattatatc ctgatatcta
      181 tcttgataac caacagcagt tattaaattt ttctctttta ttttctcaac aattttttta
      241 accttatttt tatttaaatg aattggcttt tcaacaagaa atggaatatt tttttctata
      301 cacatctcct cctgttcttc atgagcaaaa ggagggacac atatataaca ggcatctaat
      361 tcaagttgtt caagcatttt cttatagtca gtataaactt tgcctccaaa tttttcactt
      421 gctgcttttg ctttattttc atccacatca cacattccta caaatttcac tccttctatt
      481 tttgataaag cattcatatg agcatttgca attcctccac aaccaataaa actaattttt
      541 atttccattt tttatttcct ccttttaaat tttattcaaa atatttttca taaaattaaa
      601 actttttctt aaattttctt ttcttggttc aggaactcca cattcaagaa ccatataatt
      661 ttcaaaatta atcttcttaa gtgccttaaa tccactttca aaatctgtat gtcccatacc
      721 aggtaaaatt ctattactat cagcaagatg aacatgagca atccattttc ctgctctttc
      781 tattgattca gcaatatctg cttcttctat actcatatgg aaaaaatctg ccattatttt
      841 taaatatgga ctatttatct tttcacaata ttcaaccgcc tgttccaatc tgtttaaaaa
      901 atgtgtctca tacctattta atggttcaag taataaagca cattttgttt cttctgcttt
      961 ttcaactatt tctggtaatt gtgaaaatag caattctttt tcaagttcat aagcattttt
     1021 ataaggagtt aaatcaggga cttttggaga accaaaaatt ggaacaagaa ttaaaccaat
     1081 tacttccagt tctttaccta tttcaagcaa tttttttata tcttctcttg cttttgttct
     1141 gttttctttt tctgcttcaa gtaaaaaatt tctgtaacca gcacatatac ttgaaatctt
     1201 aatacctatc ttttctgaaa tttttttata ttcatcaatt ttttcaggta cctcaagtaa
     1261 tcctcttccc cacacctcta ttccttcaaa accaattttt cttgcaattt caaacttttc
     1321 acttaaatct cttccaggta aaagcccctc ctgacatcct attttcatct ttaacctcct
     1381 tttttgattt aatatctttg attttaaaaa ttaaatatag cactataaaa ttttctgtca
     1441 aattttgact gatagtattt taaaaactaa aatttttcta tgcgcgtggg tgctcattta
     1501 tggattggag aaggaattga aaaaacactg gaatactgtg aatatctggg atgtaattgt
     1561 tttcaaattt ttctttcaaa tccgagaagt tggaaaagaa aagaaaaaag catagaagaa
     1621 ataataaggt tcagagaaaa aataaaatta aaaaaaataa aaccgattgt aattcatatg
     1681 ccttacattt taaatgttgc agaaaataat attaaggaaa aaaagaaaat tgtacaactg
     1741 cttgaaaaag aaattgaaga aagtgaaaat ttaaaagctg atttttatgt tttacatccg
     1801 ggttttcata aaggaattgg agaaaaaaag ggaattgaga atgttataaa tacactcaaa
     1861 aatttttcaa tcagtaaaat caaaatatta ttagaaaata catcaggaca gggaacttct
     1921 attggatata aatttgaaca cttttcattt ctatttgaca gattaggaga taattttgga
     1981 gtgtgttttg ataccgctca tgcttttcag tcaggatata atctcaaaaa ttttgaaaaa
     2041 gttaaagaaa atattgaaaa atttatccct ttgagttata ttttattaat tcatgctaat
     2101 gattcaaaaa cagaacttaa ttcaaaaaca gacaggcatg agcatatagg aaaagggaaa
     2161 ataggaataa aaggttttga agaaattata aaagacgaat attttggaaa tttatcctat
     2221 atcatagaaa caccaaaatt aagtttgaag gaagataaaa agaatattga aattctaaaa
     2281 aaaataggag aaaaatatgg aaagatatga acctgaaaag atagaaatta aatggcagaa
     2341 aaaatgggaa gaaaataatg tttttgaagc aaaatatgaa ccaaataaaa agaaatttta
     2401 tattctcgaa atgtttccat atacttctgc acaaattcat atgggtcatg taagaaatta
     2461 tacaattgga gatgttgtcg caagatataa agttatgaat ggatataata ttttacatcc
     2521 aattggttat gatgcttttg gattacctgc tgaaaatgcg gcaataaaac agaaaattca
     2581 tcccaaagaa tggacatata ataatatttc aactataaca agacagttaa aacgacttgg
     2641 aatatcctat tgctgggaaa gagaagtaat aacctgtgac cctgaatatt acagatggaa
     2701 tcagtttttt ttcattcaat ttttcaaaag aggtcttgca tataaaaaga aagcacctgc
     2761 taactggtgt ccttcctgtg aaactgttct tgcaaatgag caggtaattg acggaaaatg
     2821 ctggagatgt ggtgaaattg tagaaaataa aaatcttgaa cagtggttta taaaaattac
     2881 tgaatatgct gaaagattac ttgatttttc tggtataaaa aactggcctg aaagagtaat
     2941 aacaatgcag aaaaactgga ttggaaaaag tgaaggatgt gaaatatatt tttctatacc
     3001 agaacttgaa gaagatatta aagtttttac aacaagaagt gatactttaa tgggtgtaac
     3061 ttatattgta ctttcaccat atcatccatt agttgaaaaa ataatgaaaa taagttccag
     3121 aaagcaacat atacaatcct ttatagataa agcaaaaaaa gcaagtttaa cagtcgctga
     3181 tattttaaaa cttgaaaaag agggagttga aacaggaata tctgcaattc atcctgtaac
     3241 acaggaaaaa atacctgtct ggattggcaa ttatgttttg atggaatacg gaacaggtgc
     3301 tgttatggct gttcctgctc atgaccagag agattttgag tttgcagaaa aatataattt
     3361 accggttaaa atagtgatta aaaatccaga ttgggaacaa atgccggaaa aaattgaaaa
     3421 ggcatatgaa ggacctggaa ttatgataaa ttcaggaatt tttgatggac ttgatagtga
     3481 aaaaggaaaa gaagaggtca ggaaatattt agaaaaacat aataaaggtt aaaaagcagt
     3541 atattataga ttgaaggact ggtgtatttc aagacagaga tactggggaa ctcctattcc
     3601 aatcatttat tgtaaaaaat gtggaattgt tacagaaaaa gaagaaaatt tacctgtcaa
     3661 acttcctgat aatgtaaaaa taacaggaaa gggagaatca cctttaaaat atgtacctga
     3721 atttttgaat tgtaaatgtc ctgtttgtgg aaatgatgca gaaagagaaa cagacacaat
     3781 ggatactttt gttgattcat cctggtattt tttaagatat gcttcaaaag atattcaaaa
     3841 taaaccattt gataaaaatg aggttaatta ctggatgcct gttgaccagt atattggagg
     3901 gatagaacat gcaattttac atttacttta cagcagattt ttcgtaaaag ttttgaaaga
     3961 tatgggatat gttgattttg aagaaccatt tgaaaattta ttatgtcagg gtatggtgat
     4021 aaaggatgga gcaaaaatgt caaaatctaa aggaaatgtg gttgaccctg aggatataat
     4081 aaaaaactat ggtgcagata ctttacggct ctttatactc tttgctgcac ctcctgaagt
     4141 tgaccttgaa tggagcgcaa aagggattga gggttgctgg agatttttaa atagaatctg
     4201 gagattacat tttgagataa aaaattatga tttacttaaa ggcaaaaatg atgaaaattt
     4261 aaaaattatg ataaataaaa cagtaaaagg agtaacagag gatatagaaa aagaatttca
     4321 atttaacacc gcaatagcaa aaatgatgga atttgttaat ttcctttaca gagaggtaaa
     4381 cgaaaaaact atttcaaaaa atttactgat tgaatcatgg aaaaaattta ttaagttact
     4441 ctatcctttt attcctcata tttgtgatga attatgggaa aatttaggtg aaaaggaatt
     4501 tttatttaat gagtcatggc cttcttatga agaaacagaa atacagggag aaaaaataaa
     4561 tataggcgtc cagataaatg gaaaaataag aggtaatatt gaaatacctt ctgagtatga
     4621 tgtggaacag atttatgagt taataaaaaa ctctgaaaaa ttcaaaaaat ttattgaagg
     4681 aaaaaatgta gtcaaaaaga tttatgttaa aaataagatt ataaatatag ttttaaaaga
     4741 ttaaccctct ttcttttcaa tttctgattc ttttttctct tcttccttct ctttttccct
     4801 cttttcttct attctatctt ttatatctga aattcctgct gcaacagcaa ttattccacc
     4861 aagtataagc ataacaacta accctccacc tataaaccat tttgttgcct gtccgagagg
     4921 acctggaaaa ataagccaca atccaatcgc tattgctatt attccaccaa ttatagcagt
     4981 cattctttac ctccttttta aaatcttcaa ttaaaaaacc acaaaaaaat tttatatcat
     5041 tataaaaatt tgtcaatttt ggtatctttt atcatctgtt ttatctgatt tattatttcc
     5101 catttgtttt caaaattcca gagtggataa atggaattat tctggaataa tgggtcagaa
     5161 aggggtaaat gaggataaag attttttgca atttcaaaat caaccgtttc aggaataggt
     5221 gaatattcag ccatttttat tttaacagga taatttttta aaacttttat tgtatttatt
     5281 acttcttttg gggtctgccc gggaagtcct gcaagtatat aaacacctat ttctttttct
     5341 gtaaatcctg catctacaag attttttata catttttcaa attcagaaaa atttacttta
     5401 taacctgatt cttcccttct cttttcctct attgtttcaa gtgaaaggcg gatagtttta
     5461 aagtttacat cttttaaaat ataagcaact tccttttcta taaattttgg atgaattcca
     5521 ttaggtgtat gaaacctcaa atttttacct gtttttttaa ttccctctaa tattttataa
     5581 agatg
//
LOCUS       DAOSAT010000105         5391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_9605_length_5391_cov_4.978261, whole genome shotgun sequence.
ACCESSION   DAOSAT010000105 DAOSAT010000000
VERSION     DAOSAT010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9605_length_5391_cov_4.978261"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PLF90_08165"
     CDS             <1..622
                     /locus_tag="PLF90_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57285.1"
                     /translation="REKGSFPNFEKSIYYKKTDALRNATRTTIAPTGTLSIIANCSSG
                     IEPIFGVAFIRNVLEGIKFLEINPVFEKIAKEENFLTPQILKEIIKTGSLKDVKGIPE
                     EYKKIFLTALDIEPIKHLKMQSIFQKYTDNAVSKTVNLPYDATIEDVKEIFINGWKMK
                     CKGITVFRYGSKTQQVLSYADTIEHELEYLILSEEMSSCSKGVCFY"
     gene            638..1654
                     /gene="amrS"
                     /locus_tag="PLF90_08170"
     CDS             638..1654
                     /gene="amrS"
                     /locus_tag="PLF90_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOK57286.1"
                     /translation="MEKARYWKEIENKKVQCQLCNHFCIIEPDKTGKCGVRLNKNGVL
                     YSLVYGEIIAENVDPIEKKPFYHFFPGSFSFSIATCGCNFFCPFCQNSEISHPPKEGR
                     IFTIRKRKPEEVVKLAKSHNCISISYTYTEPTVFGEFAFDVCKIAKEEGIYNNFVTNG
                     YMSKDFLKDISEFLDAANVDLKGDEDFYKKLPQANQKYVVENIELMRKLNIWVEVTTL
                     LIPEYNDSENQIRDIAKKIKNIDRRIPWHISRFFPAYKMSDHYPTEIEKIRKAREIGF
                     EEGLLYVYTGNIIGDEGENTYCPECKNLLIERYGYKIIKNEIKDRRCKFCRKEIDGLF
                     KFKN"
     gene            1667..2005
                     /locus_tag="PLF90_08175"
     CDS             1667..2005
                     /locus_tag="PLF90_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57287.1"
                     /translation="MKKSKVLFGLVLLFVLNLFGITDFGYKFTPDKDILLMTEENNFL
                     VQPQCIISKGNMLYFAAQDFTKNRELLIKKIKDVKVIKDELNRKILKVMYSLWDEKKG
                     IFRLFSCLIS"
     gene            2094..2270
                     /locus_tag="PLF90_08180"
     CDS             2094..2270
                     /locus_tag="PLF90_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57288.1"
                     /translation="MESAYEPYKYYSIPEKGKIMTYKLEKTKKTKIGHANWIFANTGK
                     GEGGGVNCSCCNTR"
     gene            2452..2934
                     /locus_tag="PLF90_08185"
     CDS             2452..2934
                     /locus_tag="PLF90_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57289.1"
                     /translation="MIFSDEEKNKIIEDEKVRLKIRLKYEQKSSGLAGVLSSICPGLG
                     QIYNGQFGKASSIGIIIIISIVLLSFGITFWVKGVPQKGEKVSITEEKTVEMTDEGVI
                     IEEAEKNKEKEEKKEPKSKLPYTLTILGLIGLCFTWSYAVKDAIKTSKRLNEEAIKEL
                     "
     gene            complement(2929..3552)
                     /locus_tag="PLF90_08190"
     CDS             complement(2929..3552)
                     /locus_tag="PLF90_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOK57290.1"
                     /translation="MKKYIPEDTIRRLPIYLRNLLNLQKEGKKIVSSKEITFSLNILP
                     EQFRKDLSYFGKFGKRGVGYEVDNLIKVLEKIIGIDKEIKVILVGVGRLGSALIRYKG
                     FSSMNMRIVGAFDNDTSKIGTKIDEIKIMGMEEMDGFLKKEKIKVGIICVPSDKAQKV
                     AEYMVKSGIKGILNFAPISLNLPKNIFVSYVDMASELGSLIFKLKNL"
     gene            complement(3549..4361)
                     /locus_tag="PLF90_08195"
     CDS             complement(3549..4361)
                     /locus_tag="PLF90_08195"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004399712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase subunit"
                     /protein_id="HOK57291.1"
                     /translation="MVGIDLSNFSGIVTGGAGGIGKAIVIKLLEAGADVVIADINEKE
                     GEKTIEELKEFKNKIEFIKTDVSKKYEVENLVKKTLERFKKIDYLVNNAGILIPRLLV
                     DPEGKEEITEEIFDRMVSINQKGYLLCAQSVARQMIRNKKGVIVNMASESALEGSEGQ
                     SVYAATKAAIYSFTRSWAKELGKYNIRVVGVAPGILEPTALRNPEYERALAYTRGITV
                     EELRKSYEKISIPLKRVGKLEEVANLVCFLISDLASYITGTTINISGGKSRA"
     gene            4543..5388
                     /locus_tag="PLF90_08200"
     CDS             4543..5388
                     /locus_tag="PLF90_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOK57292.1"
                     /translation="MKKAIGIDIGGTYIKAGLVNESGKILKKQQFPTLAEKNDREVVL
                     YQIEKSIEFAYEEEVEGIGIGTPGVVDDQGIVYEAPNLPSWDNLNLKKIFEDKFKKKV
                     VVENDVNTIAWGEYLFGAGKGSNTMICITLGTGLGGGIVKDGKLLRGGKYSAIEIGHI
                     TIDYKGPKCKCGNIGCIERFVGRDYIVERAIKGIKEGKKTKIYELVNGDLQKITPKTI
                     SEAYNLGDEFAESIWIDVGVCLGAMFTSLVNLLNPDVIVIGGGISQAGKILFDTVEKT
                     IKKEP"
BASE COUNT         2035 a          709 c          871 g         1776 t
ORIGIN      
        1 aagagaaaaa gggtctttcc cgaattttga aaaatcaatt tattataaaa aaactgatgc
       61 tttaagaaat gctaccagaa caacaattgc acctacagga acactcagta taattgcgaa
      121 ttgttcaagt ggaatagaac caatatttgg agttgctttt ataagaaatg ttcttgaagg
      181 aattaaattt cttgagataa atcctgtttt tgaaaaaatt gctaaagaag agaatttttt
      241 aactccccag attttaaaag aaataattaa aactggttca ttaaaggatg taaaagggat
      301 accggaagaa tacaaaaaaa tatttttaac cgctcttgat atagaaccca taaaacattt
      361 aaaaatgcaa tcaattttcc aaaaatacac ggataacgct gtttcaaaaa cagtaaatct
      421 tccttatgat gctacaattg aagatgtaaa ggaaattttt ataaatggat ggaaaatgaa
      481 atgtaaagga atcactgttt tcagatatgg aagtaaaaca cagcaggttt tatcatatgc
      541 tgatactatt gaacatgaac ttgaatactt aatattatct gaagaaatgt caagttgttc
      601 caagggagta tgtttctatt aaattcaggg gaggcaaatg gaaaaggcaa gatactggaa
      661 agagattgaa aacaagaaag ttcagtgtca attgtgtaat catttctgta taattgaacc
      721 tgataaaaca ggaaaatgtg gagtgagatt aaataaaaat ggagttttat attctcttgt
      781 ttatggcgaa attattgcag aaaatgtaga cccgattgaa aagaaacctt tttaccattt
      841 ttttcccggg agtttttcat tttcaattgc tacttgtggt tgtaacttct tctgtccttt
      901 ctgtcagaat agtgaaatat cccatcctcc caaagaagga agaattttta ccataagaaa
      961 aagaaaacct gaagaagttg ttaaacttgc aaaatcacat aattgtattt caatttctta
     1021 cacttataca gaaccaactg tttttgggga gtttgctttt gatgtatgta aaattgcaaa
     1081 agaagaagga atttataata actttgttac aaatggatat atgagtaaag attttttaaa
     1141 agatatttct gagtttcttg atgcagcaaa tgttgatttg aaaggggatg aggatttcta
     1201 taaaaaatta ccacaggcaa accagaaata tgttgttgaa aatatagaat taatgagaaa
     1261 attaaatata tgggttgagg ttactacttt attaataccg gaatacaatg atagtgaaaa
     1321 tcaaataaga gatattgcaa aaaaaataaa aaatattgat agaagaattc cctggcatat
     1381 aagcagattt tttcctgctt ataaaatgtc agaccattat ccaacagaga ttgaaaaaat
     1441 aagaaaagca agagaaattg gttttgaaga aggtctttta tatgtttata caggaaatat
     1501 tattggtgat gagggggaaa atacttattg cccagaatgt aaaaatttgc taatagaaag
     1561 atatgggtat aaaataataa aaaatgaaat taaagataga aggtgtaagt tttgtagaaa
     1621 agaaatagat ggtttattta aattcaaaaa ttaaaggggg gattatatga aaaaaagtaa
     1681 agttttattt ggattagttt tattatttgt tttaaatttg tttggaataa cagattttgg
     1741 atataaattt acacctgata aagatatcct tttgatgaca gaagaaaata attttcttgt
     1801 tcaaccgcaa tgtataattt ctaaaggaaa tatgctttat tttgctgcac aggattttac
     1861 aaaaaacaga gaattactta ttaaaaaaat aaaagatgta aaagttatta aagatgagct
     1921 aaatagaaaa atactgaaag ttatgtattc tctttgggac gaaaaaaaag gaatattcag
     1981 attattctct tgtcttattt cttgaaataa gaaaagagtt ttcatttctt gctatttatt
     2041 caaaatttat ttataacgga gaaggatcta ctgaatgcgg aataaactgg gcaatggaaa
     2101 gtgcttatga gccctataaa tattattcaa ttccagaaaa agggaaaatt atgacatata
     2161 aacttgaaaa aacaaaaaaa acaaaaattg gacatgctaa ctggattttt gctaatacag
     2221 ggaaaggaga aggagggggg gttaattgct cctgctgtaa tactcggtag aggtgagaat
     2281 tttattttta taaatagtgt tcctcctaag aaaaaattgt caaaaggtga gtcaattgat
     2341 gttttcatga tttttatacc catagaaaag aattataaaa ttcttgagga attatttcaa
     2401 aaaattaaag atataaaatg ggaatatgaa aaattttaaa aggaggtgaa tatgattttc
     2461 tctgatgagg aaaaaaataa aataattgaa gacgaaaaag taagattaaa aataagatta
     2521 aaatatgaac aaaaatcttc aggtcttgca ggtgtattat caagtatatg ccctggactc
     2581 gggcaaattt ataatggaca atttggaaaa gcatcttcta ttggaataat aataataatt
     2641 agtattgttt tactttcttt cggaatcact ttctgggtaa aaggagtgcc tcaaaaagga
     2701 gaaaaggtta gtattacaga ggaaaaaaca gttgaaatga cagacgaagg tgtaataata
     2761 gaagaagcag aaaaaaacaa agaaaaagaa gaaaagaaag aacctaaatc aaaattacca
     2821 tatactttaa caatattagg actgatagga ctttgtttta cttggagtta tgcggttaaa
     2881 gatgcgatta aaacttcaaa aagattaaat gaagaagcaa taaaagaatt atagattttt
     2941 aagtttgaag attaaagaac caagttctga agccatatca acataactaa caaatatatt
     3001 ttttggtaaa ttaagagaaa taggtgcaaa gttcaaaata cctttaattc ctgatttaac
     3061 catatattca gctacttttt gtgctttgtc agaagggaca caaattatac caactttaat
     3121 tttttctttt tttaggaaac catccatttc ttccataccc attatcttta tttcatctat
     3181 ctttgtacct attttggaag tatcattgtc aaaagcacct actattctca tattcataga
     3241 actaaatcct ttgtatctaa ttagagcaga acctaatctt ccaacaccaa caaggattac
     3301 ctttatttct ttatcaattc caataatttt ctcaagtact ttaatcaaat tgtcaacttc
     3361 atatcctact cctctctttc caaattttcc aaaatatgag aggtcttttc taaactgctc
     3421 tggtagaata tttaaagaaa atgtaatttc ctttgaagaa acaatttttt tcccttcttt
     3481 ttgaaggttt aataaatttc ttaaataaat aggtaacctt cttattgtat cctcgggaat
     3541 atattttttc atgctcttga ttttcctcct gaaatattta ttgtagttcc agttatataa
     3601 ctcgcaagat cagatattaa aaaacaaaca agatttgcta cctcttcaag ttttccaact
     3661 ctttttaatg gtattgaaat tttttcatat gactttctca attcctcaac cgttattcct
     3721 cttgtatagg caagtgctct ttcatattca ggattcctta aagcagtagg ttcaagtatt
     3781 ccaggtgcaa ctcctacaac tctaatatta tacttcccca gttcttttgc ccaacttctt
     3841 gtaaatgaat atattgcagc ttttgttgcg gcataaacac tctgtccttc agaaccttca
     3901 agggcagatt cagaagccat attaactatc acaccttttt tatttcttat catttgtctt
     3961 gcaacagatt gagcacacaa caaatatcct ttttgattta tactaaccat tctatcaaaa
     4021 atttcttcag taatttcctc tttaccctca ggatcaacaa gaagtcgcgg tatcaatatt
     4081 cctgcattgt tcactaagta atcaattttt ttaaatcttt caagtgtttt ttttactaaa
     4141 ttttcaactt catatttttt tgaaacatct gtttttataa attcaatttt atttttaaac
     4201 tcttttaatt cttcaatggt cttttctcct tctttttcat ttatatctgc aatcactaca
     4261 tctgcacctg cttcaagtaa ttttataaca attgcttttc ctataccacc cgctccacct
     4321 gtaactattc ctgaaaaatt acttaaatct atcccaacca ttctcactcc ttttttcatc
     4381 aacttaaaaa ttaaaaagat gtcctaaaaa aacttttaga accaggatct tcaggattta
     4441 aatatatcca aacattatta attttttcca tttctaatta ttctttgact aaacctgtaa
     4501 taataatata tcattatatt aaaaacttca caaggaggga agatgaaaaa agcaataggg
     4561 atagacattg gtggtactta tataaaagca gggcttgtta atgaaagcgg taaaatttta
     4621 aaaaagcagc aatttcccac acttgcagaa aaaaatgaca gagaagttgt actttatcaa
     4681 atagaaaaaa gtattgaatt tgcatatgag gaagaagttg aaggaattgg aattggaact
     4741 cctggtgttg ttgatgatca gggaattgtt tatgaagcac caaatcttcc ttcctgggat
     4801 aacttaaatc taaaaaaaat atttgaagac aaatttaaaa agaaagttgt tgttgaaaat
     4861 gatgtaaata caattgcctg gggagaatat ctttttggag caggaaaagg gagcaataca
     4921 atgatatgca taactcttgg aacaggatta ggaggaggga tagtaaaaga tggaaaactt
     4981 ttaagaggtg gtaaatattc tgctattgaa ataggtcata ttacaattga ttataaaggt
     5041 ccgaaatgca aatgtggtaa tattggatgt attgaaagat ttgtggggag agattatata
     5101 gtagaaaggg caattaaagg aattaaagaa ggaaaaaaaa ctaaaattta tgaacttgtt
     5161 aatggagacc tgcaaaagat tacccctaaa accatttctg aagcatataa tcttggcgat
     5221 gaatttgcag aaagcatatg gatagatgtt ggagtatgtt taggagcaat gtttacaagt
     5281 ttggtcaatc ttttaaatcc cgatgtaatt gtaataggtg gtggaatttc tcaagcaggg
     5341 aaaattttat ttgacacagt agaaaagaca attaagaaag agccatgaaa a
//
LOCUS       DAOSAT010000106         5334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_9731_length_5334_cov_6.384732, whole genome shotgun sequence.
ACCESSION   DAOSAT010000106 DAOSAT010000000
VERSION     DAOSAT010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9731_length_5334_cov_6.384732"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="PLF90_08205"
     CDS             <1..1310
                     /locus_tag="PLF90_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOK57293.1"
                     /translation="LSKLKILILTKNLDFYFNNPIEFSKFYIVGRLITVFYGIGVIIL
                     SFLIAKKLYKDNKRPFLISFLISFSPLIILNSSYMYVDIPGLFWIMACIYYSLKNFEN
                     LSYKNFFIAGVFSGLAMGTKIPLFTSIFIPYLTSILIYQKDIKKIFISFIYITGGAIL
                     SFGITNPYFFITFPMPLIELKQHTPLSFSGKFYINALGYGIGWPVFVCGVIGLFLNRK
                     NYIDKKYILLFLWIIFYFLFISLFSKNYARYILPITPVFIICGYEFWTEKGIKKFPRY
                     FKNIVLYLSLLFSFIYGMSFKSLFLKENIRTEAGIWIKENIPEGKSIGVCEVPWQLQM
                     PPFDYFSYKVVVINYDFEKLKKENPEYFIISSFQAPIPSYPSNLQKERIEFYQKFINS
                     NFYKPIKIFQKFPFFSIINFNFKVLPEDLIYLNPTILILKKNE"
     gene            1303..2295
                     /gene="queA"
                     /locus_tag="PLF90_08210"
     CDS             1303..2295
                     /gene="queA"
                     /locus_tag="PLF90_08210"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOK57294.1"
                     /translation="MSEIFNFDLPKELIAQYPLKEREKARLMIVHRENKEIKEDLFEN
                     IGNYLNDRFVIVLNDTRVIPARIICNKETGGKIEVFLLKNLDKFLWQVLIKGKIKVSA
                     KFKKEDMEGKIVEKKENGSYIVEFNKDKEEIKNYGEIPLPPYIKRKPEKEDKIYYQTV
                     YAKKNGSIAAPTAGLHFTEEIIEKLKNKGIEFISITLHIGWYSIKVLKENKNSVGEEY
                     LEINEETAIRLNELKSKGKKIIAVGTSTTRGLESATENGKIKPYNGYTNLFIKPGFKF
                     KFIDGLITNFHLPKSTHLSLVCAFAGTELIEKCYKIAIEKKYRFYSYGDSMLII"
     gene            2297..3415
                     /gene="tgt"
                     /locus_tag="PLF90_08215"
     CDS             2297..3415
                     /gene="tgt"
                     /locus_tag="PLF90_08215"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOK57295.1"
                     /translation="MEFKILYKNLETKARIGEIKLPHGKVKTPCFMPVATLANVKTLT
                     SEDLNEIGVEMIISNAYHLYLKPGVEIIEKIGGIHKFMNWEKPIVTDSGGFQIFSLEN
                     VKINEDGVIFKSKIDGSTHFITPEKSIEIQEKIGADIIMAFDYCPKNWNDYEEVKKSV
                     DLTLKWANICKKVHKKENQELFGIIQGGIYNELREKCIEGLEKIGFGGYGFGGLSIGE
                     PYEETLKTTKYIIENLPYNKIRYFMGIGMPLQILELVEMGIDMFDCGMITHIARTGST
                     LTWKGKINIKAGKYKDDFSPLDPDCDCIVCKNYTKSYIRHLINTKEITGLRLNSFHNV
                     YFMIKFMEKIRESIKNNNFKKFKKEFINEWKREMEQDF"
     gene            3381..4499
                     /locus_tag="PLF90_08220"
     CDS             3381..4499
                     /locus_tag="PLF90_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK57296.1"
                     /translation="MNGRERWNRTFNFQKVDRIFNCEFGWWNETLLRWHKEGLPEEVK
                     TNGDGDKFFKFDPIGGIPINMGLMPNFEEKIIEETERYIIKQGTDGVISKIFKNEIQT
                     IPHYIKFPVENKNDWIDFKERLKPEISKRYPDEKTWENLKKEWKNRDYPLGIGVGSLF
                     GWIRNWMGFENCLISIYEQPDLIEEMMDHIVYFVLTLIEKALEEVKDIDFAAFWEDMA
                     FKTGPMISPKHFSQFMVPRYKKITERLKKEGINVVIVDCDGNINELVSLWLEAGVNVM
                     FPLEINSNSDPVVLRKKYGNKVLLKGGFDKKALIYGKEAIDKEFERIKEVVNSGGYIP
                     HVDHRVPADVSYGNYIYYLKKKKEVFNIFDWEIEEILK"
     gene            4496..>5334
                     /gene="era"
                     /locus_tag="PLF90_08225"
     CDS             4496..>5334
                     /gene="era"
                     /locus_tag="PLF90_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOK57297.1"
                     /translation="MKCGYVTILGKPNTGKSTLLNTILKQKVSIVSPKPATTRIKVIG
                     IYNDERGQIIFIDTPGFEEGRNELGKFMLKSIYSSLEETNLILFLIESTGWTNEDEKI
                     LEILKKLENKKVILGINKIDILKFKEELLPLIDESSKKFNFVEIVPFSALKNKNLDSL
                     IKAIFSHLPESEKFYPVDMITNLPLEYKLAEIIREKVLQKTFQEVPHSVAVEIEEMKE
                     GEKNKDMVVIKANIIVDRENLKSIIIGKDGKMIKTIGKLAREEMELILGKKIYLELWV
                     KVI"
BASE COUNT         2127 a          552 c          879 g         1776 t
ORIGIN      
        1 ttctatcaaa attaaaaatt cttattctta caaaaaacct tgatttttat tttaataatc
       61 ctatagaatt cagcaaattt tacattgttg gtagattaat tactgttttc tatggcattg
      121 gtgtaataat tttgtctttc ttaattgcta aaaagttata taaggataat aaaaggccat
      181 ttttgataag ttttttaatc tctttttcac ctttgataat acttaattca tcctatatgt
      241 atgttgatat tcctggactt ttctggatta tggcatgtat atattattct cttaaaaatt
      301 ttgaaaattt atcttataaa aattttttta ttgctggagt tttttctgga cttgccatgg
      361 gaaccaaaat ccctcttttt acttcaattt ttattccata tttaacctca attttaattt
      421 atcagaaaga tattaaaaaa atttttattt cttttattta tattacagga ggagcaattt
      481 tgtcttttgg aataacaaat ccttactttt ttataacttt cccaatgcca cttattgaat
      541 taaaacagca tacaccttta agtttcagtg gaaaatttta tataaatgct cttggatatg
      601 ggattggatg gcctgtattt gtatgtggag taattggttt gtttttaaac agaaaaaatt
      661 acattgataa aaaatatatt ttgctttttc tttggattat cttttatttt ttattcatct
      721 cacttttctc taaaaattat gcaagatata ttttaccaat cacaccagtt tttataattt
      781 gtggatatga attctggact gaaaaaggaa taaaaaaatt cccaagatat tttaaaaaca
      841 ttgttttata tctatccctt cttttttctt ttatttatgg tatgagtttt aaatcattat
      901 ttttaaagga aaatataagg acagaagcag gaatatggat aaaagaaaat ataccagaag
      961 gaaaatctat tggtgtttgt gaagttccat ggcagcttca gatgcctcct tttgattatt
     1021 tttcttataa agttgttgtt ataaattatg attttgaaaa attaaaaaaa gaaaatcctg
     1081 aatactttat aatttcttct tttcaggcac caataccatc ctacccttca aatttacaaa
     1141 aagaaagaat agaattttac cagaaattta taaactcaaa tttttataaa ccaataaaaa
     1201 tctttcaaaa atttcctttt ttcagtataa taaattttaa ttttaaagtt ttgcccgaag
     1261 atttgatata cttaaatcca acaattttaa tattaaaaaa aaatgagtga gatatttaat
     1321 tttgatttac caaaagaatt gattgctcag tatccattaa aagaaagaga aaaagcaaga
     1381 ttgatgattg tccataggga aaataaagaa attaaagaag acctttttga aaatattggg
     1441 aattatttaa acgatagatt tgtaattgtt ttaaatgata caagagttat accagcaaga
     1501 ataatctgca ataaagagac gggtggaaaa atagaagtat ttcttttaaa aaatcttgat
     1561 aaatttttat ggcaggtact tattaaagga aaaataaaag ttagtgcgaa atttaaaaaa
     1621 gaagatatgg aaggaaagat tgttgaaaaa aaagaaaatg gaagttatat tgttgaattt
     1681 aataaggata aagaagaaat taaaaattat ggagaaatac cactacctcc ttatataaaa
     1741 aggaaaccag aaaaagagga taaaatttat tatcaaactg tctatgcaaa gaaaaatggt
     1801 tcaattgcag caccgacagc aggacttcat tttacagaag agattattga aaaattgaaa
     1861 aataagggaa ttgaatttat atcaattact ttacatattg gatggtattc aataaaggtc
     1921 ttaaaagaaa ataaaaattc tgttggtgag gaatatttag aaataaatga agaaactgct
     1981 ataagattga acgaacttaa aagtaaaggt aaaaaaatta ttgctgttgg aacaagtaca
     2041 acaagaggtc ttgaaagtgc aactgaaaat ggaaagatta aaccatataa tggctataca
     2101 aatcttttta taaaaccagg ttttaaattt aaatttattg atggacttat aacaaacttt
     2161 cacctgccaa aatctaccca cttatctctt gtctgtgctt ttgctggaac tgaattgatt
     2221 gaaaagtgtt ataaaattgc tattgaaaaa aaatatagat tttattctta tggtgatagt
     2281 atgcttataa tttaaaatgg aattcaaaat tttatataaa aatttagaaa cgaaagcaag
     2341 aattggagaa ataaaacttc cacatggaaa agttaaaaca ccttgtttta tgcctgttgc
     2401 aacacttgca aatgtaaaaa ctcttacttc tgaggactta aacgaaattg gtgttgaaat
     2461 gataatctca aatgcatatc atctttatct taagccaggg gttgaaatta ttgaaaaaat
     2521 aggtggtatt cataaattta tgaattggga aaaaccaatt gttactgata gtggtggatt
     2581 tcaaatattt agtttagaaa atgtgaaaat aaatgaagat ggagtaattt ttaaatcaaa
     2641 gatagatggt tcaacccatt ttataacacc agaaaaatca atagaaattc aggaaaaaat
     2701 aggagcagat ataataatgg cttttgatta ttgtccaaaa aactggaatg attatgagga
     2761 agtaaaaaaa agtgttgatt tgactttaaa atgggccaat atatgtaaaa aagtgcataa
     2821 aaaagaaaat caggaattat ttggtattat tcagggaggt atttataatg aattaaggga
     2881 aaaatgtatt gaaggactgg aaaaaattgg ttttggtgga tatggctttg gtggtttgag
     2941 tattggagag ccatatgaag aaacattaaa aacaacaaaa tacataattg aaaatttacc
     3001 ttataataaa atcagatatt ttatgggaat tggaatgcct ttacaaatac ttgaacttgt
     3061 tgaaatggga attgatatgt ttgattgtgg tatgataaca catattgcaa ggactggttc
     3121 aacattaact tggaaaggaa agataaatat aaaggcaggt aaatataaag atgatttttc
     3181 tccacttgac cctgattgtg attgtattgt ctgtaaaaat tatacgaaaa gttatataag
     3241 acatcttata aatacaaagg aaataacagg ccttcgcctt aattcttttc acaatgttta
     3301 ttttatgatt aaatttatgg aaaaaattag agaaagcata aaaaataata actttaaaaa
     3361 attcaaaaag gagtttataa atgaatggaa gagagagatg gaacaggact tttaattttc
     3421 aaaaagttga tagaatattt aattgtgaat ttggatggtg gaatgagaca cttttaagat
     3481 ggcataaaga aggattacca gaagaagtaa aaacaaatgg agatggagat aaattcttta
     3541 aatttgaccc tatcggagga atacctataa atatgggttt aatgccaaat tttgaagaaa
     3601 aaataattga agaaacagaa agatacataa taaaacaggg aacagatgga gttataagta
     3661 aaatatttaa aaatgaaatt cagacaattc cccattatat aaaatttcct gttgaaaata
     3721 aaaatgactg gattgatttt aaagaaagat taaaacctga aatttcaaaa agatatcctg
     3781 atgaaaaaac atgggaaaat ttaaaaaaag aatggaaaaa tagagattat cctcttggaa
     3841 ttggtgttgg ttctttattt ggctggataa gaaactggat gggatttgaa aactgcctta
     3901 tttctattta tgaacaacct gacttaatag aagaaatgat ggaccatatt gtttattttg
     3961 ttttaacatt aattgaaaag gcacttgaag aagttaaaga tattgatttt gctgcctttt
     4021 gggaagatat ggctttcaaa acaggaccaa tgatttctcc aaaacatttt tctcaattta
     4081 tggttccaag atataaaaaa attactgaaa gattgaaaaa agaaggaata aatgttgtaa
     4141 tagttgattg tgatggaaat ataaatgaac tagtatcttt atggcttgag gcaggggtaa
     4201 atgtgatgtt tcctcttgaa ataaatagta attctgaccc tgttgtttta agaaaaaaat
     4261 atgggaataa agttcttttg aaaggtggtt ttgataaaaa ggcattaata tatggaaaag
     4321 aggcaattga taaagaattt gaaagaataa aggaagttgt taattcagga ggttatattc
     4381 cacatgttga ccacagggtt cctgctgatg taagttatgg gaactatatt tattacttaa
     4441 aaaagaaaaa agaagttttc aatatttttg attgggaaat agaggagata ctgaaatgaa
     4501 atgcggatat gtaacaatac tcggaaagcc aaatacagga aaatcaactt tacttaatac
     4561 tattttgaaa cagaaggtct ctattgtctc tcctaaacca gcaactacaa ggattaaagt
     4621 tataggaatt tataatgatg aaagaggcca gataattttt atagatacac ctgggtttga
     4681 agaaggaaga aatgaactcg ggaaatttat gttaaagtca atatattcat ctcttgaaga
     4741 gacaaacctc attttatttt taattgaaag caccggctgg acaaatgaag atgaaaaaat
     4801 tcttgaaatt ttaaaaaaac tggaaaataa aaaagtcata cttggaatta ataagataga
     4861 tatacttaag tttaaagaag aacttcttcc tttgattgat gaaagtagta aaaagtttaa
     4921 ttttgttgaa atagtccctt tttctgcatt aaaaaataaa aatcttgatt cattaattaa
     4981 agcaattttt tcacatcttc ctgaatcaga aaaattttat ccggttgata tgataacaaa
     5041 cttaccactt gaatataaac ttgcagaaat aataagggaa aaagtattac agaaaacatt
     5101 tcaggaagtt cctcattctg ttgctgttga aattgaagaa atgaaagaag gagaaaaaaa
     5161 taaggatatg gttgtaatca aagcgaatat aattgtggat agggaaaatt taaaatcaat
     5221 tattattggg aaagatggaa aaatgataaa aacaatcggt aaacttgcaa gagaagagat
     5281 ggagttaatt ttagggaaaa agatatatct tgaattgtgg gttaaagtaa ttga
//
LOCUS       DAOSAT010000107         5155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_10131_length_5155_cov_4.519804, whole genome shotgun sequence.
ACCESSION   DAOSAT010000107 DAOSAT010000000
VERSION     DAOSAT010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10131_length_5155_cov_4.519804"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="PLF90_08230"
     CDS             <1..129
                     /locus_tag="PLF90_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOK57298.1"
                     /translation="GITVYGKGVSTDIIEASGKAYLNAINFYLVRKETGKKIIKGA"
     gene            131..1387
                     /gene="leuC"
                     /locus_tag="PLF90_08235"
     CDS             131..1387
                     /gene="leuC"
                     /locus_tag="PLF90_08235"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOK57299.1"
                     /translation="MTISQKILAKHCGREEVFPSEYVECSVDLILANDITGPLAIEEF
                     EKVGAENVFEPSKIVLVPDHFTPCKDIKSAELVKKIRDFSKKYGVKFYEIGSVGIEHA
                     LLPEEGLTLPGQLIIGADSHTCTYGAVGAFSTGVGSTDVAYAIITGKLWFKVPETIKF
                     YYYGKLNDYVGGKDLIIYTIGLIGVDGANYKSMEFTGPVIENLPIDDRFTMCNMAIEA
                     GGKNGIIKPDRITEEYLKEIGLDIKIDENLKTDSENDYEKIIEIDVSKIEPQVSAPHL
                     PSNSKSVREYSNVKIDQVVIGSCTNGRISDLRKAAKILKGKKIHPEVRCIIIPATQKV
                     YKQALKEGLIDIFIESGCVISPPTCGPCLGGHMGVLAKGEVAIATTNRNFIGRMGHPE
                     SYVYLSNPEVAAASAVKGKISHPEEV"
     gene            1388..1882
                     /gene="leuD"
                     /locus_tag="PLF90_08240"
     CDS             1388..1882
                     /gene="leuD"
                     /locus_tag="PLF90_08240"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013748807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOK57300.1"
                     /translation="MKVKGKVWKFGDHINTDDIIAARYLNTTDEKELASHCFEVIRPE
                     FSKNVKKGDIIVAGENFGCGSSREHAPISIKGCGVSAVIAKSFARIFFRNSINIGLPL
                     IELKEVDEIEEGEELEIDFDEGIIKNLNTGKTYKFQKYPEFLQNIINSGGLMSLARKT
                     VEKK"
     gene            1879..3162
                     /locus_tag="PLF90_08245"
     CDS             1879..3162
                     /locus_tag="PLF90_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine-DL-glutamate epimerase"
                     /protein_id="HOK57301.1"
                     /translation="MKILETNLNFEIEEFKKPLGFKGKFLKGAWQVITYFKSENNFSI
                     GLGTQSVLWSDNNIFNENSEAGGNALMFATTQYAIKLLKNREIENPVKILEEIFEDVH
                     NYAKKITARKDLRETFTLNSLVSVDNALWILYAKEKNICDFLEMIPEEYRLPLKYRHK
                     KIACIPLISYAVSIEEIEKMIKEGFFVLKIKIGSDPDNDGDREKMLKWDMERIKKIHE
                     KFKNYETPYTENGKLPYYFDANGRYDSKDRLKKLIDFIDKIGALEQVIIIEEPFPEEL
                     EIDVKDIPVRIVADESAHTDKDVIKRRQMGYKGVALKPIAKTLSMTLKIINEAEKNNI
                     DYFCADLTVSPFIAEWNKNLAVRIKLFPELKIPFFESNGFQYYKNWGKLLKYHPCYGK
                     KWISPENGIFNLDDEFYKLSGGIFETPKHYKNLVI"
     gene            3177..4250
                     /locus_tag="PLF90_08250"
     CDS             3177..4250
                     /locus_tag="PLF90_08250"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOK57302.1"
                     /translation="MRKKYKIGVIGGDGIGPEIIKEGMKVVESVSEVCEFKVEWVEYD
                     FGGDRYLKTGETLPDSAIEEFKKLDSIYLGAVGHPDVPPGILEKGILLKIRFELDQYI
                     NLRPVKLYPGVDCPLKNKKPEDIDYVVVRENTEGLYCGIGGNFKKGTPDEIATQLSIN
                     TRKGIERCIRYAFKLAEKRNKKKTLTVVDKANVLTYEGDLWRRTATELLREFPDIKLD
                     FAFVDATCMWMVKNPEWFDVIVTNNMFGDIITDLGAITQGGLGIAAGGNINPEGVSMF
                     EPIHGSAPKYAGKNVACPLATISAGGMMLEQLGEIKASELIEKAIIKALSEGYIKSLE
                     AGKMGMSTSEVGDLISKFVKEVK"
     gene            4316..4687
                     /locus_tag="PLF90_08255"
     CDS             4316..4687
                     /locus_tag="PLF90_08255"
                     /inference="COORDINATES: protein motif:HMM:NF023036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HOK57303.1"
                     /translation="MRKFITTLSCILFLTSMGISLFAKEGKEKGEKKEKPKVDIKHAY
                     GEITKLDLENYSVTIKQDDGTEITLKATTEKTKEMLKNLKVGDKVKAVYFEKDGELVI
                     GKIGIPKSDGKKEHKKEKLTQ"
     gene            complement(4721..>5155)
                     /locus_tag="PLF90_08260"
     CDS             complement(4721..>5155)
                     /locus_tag="PLF90_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57304.1"
                     /translation="LLKENFTDIIVYPKLLNLNYGIDFQIKLVEEARNGKSDGIVIGN
                     RIFNEGINSAWGGTELREKNLKVIKEVKRIFPEFCISATGGVYTGRHMLDYLNAGAEN
                     VQILSYILGKVKKQFLKQKGDKFEKVLYELFFHPENGFLIFF"
BASE COUNT         2015 a          609 c          982 g         1549 t
ORIGIN      
        1 ggaattactg tttatggaaa aggtgtaagt acagatataa ttgaagcaag tggaaaagca
       61 tatttaaatg caataaattt ttatcttgta agaaaagaga caggaaaaaa gataatcaaa
      121 ggagcataaa atgacaatat cccagaaaat tcttgcaaaa cattgtggaa gagaagaagt
      181 ttttccttct gaatatgtgg aatgcagtgt tgatttaatt cttgccaatg acataacagg
      241 acctcttgct attgaagaat ttgaaaaagt tggtgctgaa aatgtttttg aaccttcaaa
      301 gatagttctt gtaccagacc attttacacc atgtaaggat attaaaagtg ctgaacttgt
      361 aaaaaaaata agagactttt caaaaaaata tggcgttaaa ttttacgaaa ttgggagtgt
      421 tgggattgaa cacgcacttt taccggaaga aggactgact ttaccaggac agttaattat
      481 tggtgcggat agccatacct gtacatatgg agcagttgga gcattttcta caggagttgg
      541 aagtactgat gttgcttatg caataataac aggaaaattg tggtttaaag taccagaaac
      601 tataaaattt tattattatg ggaaactgaa tgattatgtt ggtggaaaag atttaattat
      661 ttacacaatc ggtttaattg gtgttgatgg agcaaattat aaaagtatgg aatttacagg
      721 tcctgttatt gaaaatttac caattgatga tagatttaca atgtgtaata tggctatcga
      781 ggcaggtggg aaaaatggaa taataaaacc tgacagaatc acagaagaat atttgaaaga
      841 aataggttta gatataaaaa ttgacgagaa tttaaaaaca gacagcgaaa atgattatga
      901 aaagataata gagatagatg ttagtaaaat tgagccgcag gtttctgcac cccatcttcc
      961 aagcaattca aaaagtgtaa gagaatattc aaatgttaaa attgaccagg tagttattgg
     1021 ttcatgtaca aatggaagaa taagtgattt aagaaaggca gcaaaaattc ttaaaggtaa
     1081 aaaaattcat ccagaggtaa gatgtataat aattcctgca actcaaaaag tatataaaca
     1141 ggcattaaag gaagggttaa ttgatatatt tattgaatct ggttgtgtta tttctcctcc
     1201 tacctgtggt ccttgtcttg gcggtcatat gggagttctt gcaaaaggtg aagtggcgat
     1261 agcgactacg aacagaaatt ttataggaag aatgggacat cctgaaagtt atgtatatct
     1321 ttccaatcct gaagttgctg ctgcaagtgc tgtaaagggt aaaatatctc atccagagga
     1381 ggtataaatg aaagtaaaag gaaaagtatg gaaatttgga gaccatataa atacagatga
     1441 tataattgca gcaagatatt taaatacaac tgatgaaaaa gaacttgcaa gtcattgttt
     1501 tgaagtaata agacctgaat tttcaaaaaa tgtaaaaaaa ggagatatta ttgttgctgg
     1561 agaaaatttt ggctgtggta gtagtagaga gcatgcacca atttcaatta aaggatgtgg
     1621 tgtttctgct gttattgcta agagttttgc aagaatattt ttcagaaact caataaatat
     1681 aggacttcct ttaattgaac ttaaagaggt agatgaaata gaagaaggcg aagaacttga
     1741 aattgatttt gatgaaggaa ttataaaaaa tttaaatacc ggaaaaacct ataaatttca
     1801 aaaatatcct gaattcttgc aaaatataat aaacagtggt ggattaatgt ccctcgccag
     1861 aaaaacagtt gaaaaaaaat gaaaatactt gaaacaaatt taaattttga aatagaagaa
     1921 tttaaaaaac cacttggatt taaaggaaag tttttaaaag gagcgtggca ggtaataaca
     1981 tattttaaaa gtgagaataa tttttctata ggtcttggaa ctcaaagtgt tttatggtct
     2041 gataataata tcttcaatga aaacagtgaa gcgggtggaa atgcattgat gtttgccaca
     2101 actcaatatg ccataaaact attaaagaat agagaaattg aaaatcctgt aaaaatctta
     2161 gaagaaattt ttgaagatgt ccacaattat gccaaaaaaa taacagccag aaaagattta
     2221 agagaaacat ttactttaaa ttctcttgtt agtgttgata atgctttatg gattctttat
     2281 gctaaggaaa aaaatatctg tgattttctg gaaatgattc ctgaagaata tcgtttacct
     2341 ttgaaataca gacataaaaa aattgcctgc attcctttaa tttcctatgc tgtttcaatt
     2401 gaagaaatag aaaaaatgat aaaagagggt ttttttgtcc tgaaaattaa aattggttct
     2461 gaccctgata atgatggaga cagggaaaaa atgcttaaat gggatatgga gagaataaag
     2521 aaaattcatg aaaaattcaa aaactatgaa accccttata cagaaaatgg aaaattaccc
     2581 tattattttg acgcaaatgg tagatatgat tcaaaggata gattaaaaaa attaatagat
     2641 tttatagata aaatcggtgc acttgaacag gtaataataa tagaagaacc atttccagaa
     2701 gagttagaaa tagatgttaa agatatccct gtaagaattg ttgccgacga aagtgcacat
     2761 actgataaag atgttataaa aagaagacag atgggatata aaggtgttgc attaaaacca
     2821 attgcaaaaa ctctgagcat gactttgaaa attataaatg aagcggagaa aaataatatt
     2881 gattattttt gtgctgacct tactgtaagc ccattcattg cggaatggaa taagaattta
     2941 gcagtaagaa taaagttatt tcctgaatta aaaataccat tttttgaatc aaatggattt
     3001 cagtattaca aaaactgggg aaaactttta aaatatcatc cttgttatgg aaaaaaatgg
     3061 ataagtcctg aaaacgggat atttaatctt gatgatgaat tttataaatt aagcggtggt
     3121 atatttgaaa caccaaaaca ttataaaaat cttgttattt aaagaaagga gcaaaaatga
     3181 ggaaaaaata taaaatagga gttattggag gtgatggtat cggtcctgaa attataaaag
     3241 aaggaatgaa ggttgttgaa agtgtaagtg aggtctgtga atttaaagtt gaatgggtag
     3301 aatatgattt tggtggagat agatatttga aaacaggaga aacattacct gatagtgcaa
     3361 ttgaagaatt taaaaaactt gattctattt atcttggagc agttggtcat cctgatgttc
     3421 ctccaggaat tcttgaaaaa ggaatacttc ttaaaatcag atttgaactt gaccagtata
     3481 taaatttaag acctgtaaaa ctttatcctg gagttgattg tcctttaaag aataaaaaac
     3541 cagaggatat tgattatgtt gttgtaaggg aaaatacaga aggactatac tgtggaattg
     3601 gagggaattt taaaaaggga acaccagatg aaattgcaac tcaactatca ataaatacaa
     3661 ggaaaggcat tgaaagatgt ataaggtatg cttttaaact tgcagaaaaa agaaataaaa
     3721 agaagacatt aacagttgtt gataaggcaa atgttttaac ttatgaggga gatttatgga
     3781 ggagaactgc aacagaatta ttaagagaat ttcccgatat taaacttgac tttgcttttg
     3841 ttgatgctac atgtatgtgg atggtaaaaa atcctgaatg gtttgatgtt atagttacaa
     3901 ataatatgtt tggagatata attacagacc ttggagcaat aactcagggt ggacttggaa
     3961 tagcggctgg tggaaatata aatcctgaag gagtaagtat gtttgaacca attcatggtt
     4021 ctgcaccgaa atatgctgga aaaaatgttg cctgtccact tgcgactata tctgcaggcg
     4081 gaatgatgct tgaacaactg ggtgagataa aagcatctga acttatagaa aaagcgatta
     4141 taaaagcatt atcagaaggt tatattaaat cacttgaggc gggaaaaatg gggatgagta
     4201 caagtgaagt tggtgattta atatcaaaat ttgtcaaaga ggtaaaataa actttttata
     4261 tattttaatc gtccaaattt tggactggca ccgaataaaa aggaggtgag gcatgatgag
     4321 aaaatttata acgacattat cctgtatttt atttcttacc tcaatgggaa tttctctttt
     4381 tgctaaagaa gggaaagaaa agggagaaaa gaaagaaaaa ccgaaagttg atatcaaaca
     4441 tgcatatggt gagataacca aattagattt agaaaattat tcagtaacaa taaaacagga
     4501 tgatggaaca gaaataacat taaaagcaac aacagaaaaa actaaggaga tgctgaaaaa
     4561 tttaaaagtc ggagataagg taaaagcagt atattttgaa aaagatggag aacttgtaat
     4621 tgggaaaatc ggaataccaa aaagtgatgg caaaaaagaa cataaaaagg aaaaactaac
     4681 tcaataataa cttttttctt ctttggtgcc agtccacttt ttaaaaaaat atcaaaaaac
     4741 cattttcagg atggaaaaag agttcataaa ggactttttc aaatttatca cccttttgtt
     4801 ttaaaaattg ttttttgact tttccgagga tataacttaa aatctggaca ttttcagcac
     4861 ctgcatttag ataatcaagc atatgtcttc ctgtataaac tccacctgtt gcagaaatac
     4921 aaaattcagg aaatattctt tttacttcct ttattacttt taaatttttt tccctcaatt
     4981 cagttcctcc ccatgctgaa tttatccctt cattaaaaat cctatttcct ataacaatac
     5041 catcactttt tccatttctt gcttcttcaa caagttttat ctgaaaatca ataccataat
     5101 taagatttaa aagttttgga taaacaatta tatcagtaaa attttcttta agtaa
//
LOCUS       DAOSAT010000108         5099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_10279_length_5099_cov_4.910785, whole genome shotgun sequence.
ACCESSION   DAOSAT010000108 DAOSAT010000000
VERSION     DAOSAT010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10279_length_5099_cov_4.910785"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLF90_08265"
     CDS             <1..316
                     /locus_tag="PLF90_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOK57305.1"
                     /translation="VAIVDRNWLCVTDFTENIVKLEPLEEKWESYGWETERINGHDFG
                     EIFKVLKNVKERKSEKPLVIIANTIKGKGVSFMENQILWHGMAPKGKEAEKAKKELNN
                     EK"
     gene            306..1241
                     /locus_tag="PLF90_08270"
     CDS             306..1241
                     /locus_tag="PLF90_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOK57306.1"
                     /translation="MKNKTMRDSFFDRLYDLAKKDKNIYLISADMGAPSLDKYRRDLK
                     EQFINVGIAEENMVLIATGLSLCGKKAYIYAIMPFVTSRCYEMLKVDLSLMNIPVTAI
                     GVGAGFGYDDSGPTHHCTEDITIMRVLPNFYIFSPSDSLMAYKFAEITYKLKSPSYVR
                     LDRKILPLIYKEDNDFSDGLSVLKEGKDFYIIGTGNMTHRAIEVAEELEKYRINAGVI
                     DLYRIKPINEQLLIKIVKNTKGVVTIEEHLLAGGMGSAVIEVLMDNGINLPVKRFGVK
                     DRYLYAYGGRENIQKICGLDKESVVNGILEWTGKI"
     gene            1223..2155
                     /locus_tag="PLF90_08275"
     CDS             1223..2155
                     /locus_tag="PLF90_08275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK57307.1"
                     /translation="MDRKNLISIVFSFRNEEENLSELIKRTKNAIEKTNYDYEIIFVN
                     DASTDNSLEILKEYAIEDRRIKIINLSRRFGYNQGLIAGLNYSKGDAIITLDADLQDP
                     PELIPELIKKWENGADIVHTIRKKREGDSKIKVILIKIAYKIINFVSEIRLPQDAGNF
                     KLISRKALNEVLKLKEKDPYIRGLVVWAGFKQDYIYYIREKRAKGKTHFPFLRSMGPF
                     MEFISGVTSFSILPLLYIVVLGLILIFLGIVFFILSIFHLIYFWISFMVFFSGIIIFS
                     NGILGVYIGRIWKEVLNRPNYIVESTINIEENKY"
     gene            complement(2158..3771)
                     /locus_tag="PLF90_08280"
     CDS             complement(2158..3771)
                     /locus_tag="PLF90_08280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOK57308.1"
                     /translation="MKNVFEILILSFFIFFSGYFPARFLTQNLPFEKEEKFILNFAFS
                     FFLFYIVGFLSYVFNSNFAIFNGIFLLIFLFFSFFYILKNGFDNQEIKFLKNFFIIFL
                     VFILYQTLIPLYFGGLFYWDWFEHFLRSIFFLDRLPKDIVFEGYIIPSRPPFFNIVCF
                     FYLSITGGEFYKYQIVSTFLNSMLILSVYLFCKEYLKIENKYLFLIVCVVMFLNPAIL
                     RQITYTWTKAFCAFYVILGLYFYLKFLKNQNSLHLFISSFLFGIGFIVHFSAGCYIVP
                     VFLHLAFRTILNKNLIKKTGISYLIFLFVIFTYFSWSIKNYGVYKSFFSTNIYQQQKN
                     ISLIDRIEKDSFNFIKTLHPFPLKYYKYFFNNSPLFYKFFNYLHPAYVCTLPGSLTFS
                     LTFFLLILFLKNIKQINLKTLSSHSFLIFFFFLSYLLDIVVMPLKELSGLAHTGLLPL
                     TCLFICFGIKYILSMPEKNFFIFSSVFLIETLFILTVFTIAFNIYSDPQRIISLGMSE
                     RIDSSLISNFLLKYYNHLVFLHDKISIFK"
     gene            3865..4797
                     /locus_tag="PLF90_08285"
     CDS             3865..4797
                     /locus_tag="PLF90_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOK57309.1"
                     /translation="MKILVTGGGGYIGSVLVPILLQKGYEVIVLDNFMYNQTSLLDCC
                     HLKNLEIIRGDTRDENLLKEILKEVDVIFPLACITGAPACDRNPYEAKSVNLDAIKLI
                     LKLRSKEQIIIFPTTNSGYGIGQEGIYCTEETPLRPISLYGRLKVEAEKAVLDSGNSI
                     TLRFATCFGISPRMRLDLLVNDFVYRAVVDRYLVVYQGHFKRNYIHVRDASFSFIHCL
                     ENFEKMKNNTYNVGLSDANISKLELCELIKKHIPDFYYHEAEVGEDPDKRNYIVSNEK
                     IEKTGYKAKISLDEGINELIKGYKIIRKYGFSNV"
     gene            4806..>5099
                     /locus_tag="PLF90_08290"
     CDS             4806..>5099
                     /locus_tag="PLF90_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOK57310.1"
                     /translation="MIDLLLVNPGNPKKVYGKLRSNLAGIEPPYWCALIASFIRKKGY
                     SVKIIDADAENLSPEETVNEIIKNNPVLVNIVVMGTNPSASSSPKMSTVRELLN"
BASE COUNT         2116 a          565 c          812 g         1606 t
ORIGIN      
        1 tgttgctatt gttgacagaa attggttatg tgttactgat tttacagaga atatagttaa
       61 acttgaacca ttagaagaaa aatgggaaag ttatggatgg gaaactgaaa gaataaatgg
      121 ccatgatttt ggtgaaatat ttaaagtatt aaaaaatgta aaagaaagga aatctgaaaa
      181 accccttgtt attattgcaa atacaattaa aggcaaaggt gtttctttca tggaaaatca
      241 gattttatgg catggtatgg caccaaaagg aaaagaagca gaaaaagcaa aaaaggagtt
      301 aaataatgaa aaataaaact atgagagatt ctttttttga ccgtctttat gatttagcaa
      361 aaaaggataa aaatatttat ttaatttctg ctgatatggg agcaccttct cttgataaat
      421 atagaagaga tttaaaagag cagtttataa atgttggaat tgcagaagaa aatatggtcc
      481 taattgcaac agggttatct ctgtgtggaa aaaaggcata tatatatgca ataatgcctt
      541 ttgtaacttc aagatgctat gagatgttga aagttgattt atctctgatg aatattcctg
      601 ttactgccat tggagttggt gctggttttg gttatgatga ttcaggacct actcatcatt
      661 gtacagaaga tataacaata atgagagttt tacctaattt ttatattttc tctccatcag
      721 atagtttaat ggcttataaa ttcgcagaaa ttacttataa attaaaatct ccttcttatg
      781 tacggcttga tagaaaaatt ctacctttaa tatataaaga agataatgat ttctcagatg
      841 gactttctgt tttaaaagaa gggaaagatt tttatataat tggaactgga aatatgacac
      901 acagagcaat tgaggtagca gaagaacttg aaaaatacag aataaacgca ggagtgattg
      961 atttatacag aataaaacca ataaacgaac aattactcat aaaaatagtt aaaaatacaa
     1021 aaggagttgt aactattgaa gaacatttgc ttgcaggtgg aatgggaagt gctgttattg
     1081 aagttttgat ggataatgga ataaatttac ctgtaaaaag atttggagta aaagatagat
     1141 atctttatgc ttatggtgga agagaaaaca tccagaaaat atgcggactg gacaaagagt
     1201 cagtcgttaa tggaattctt gaatggacag gaaaaatttg atttctattg ttttttcttt
     1261 tagaaatgaa gaagaaaatc tttctgaatt gataaaaaga acaaaaaatg caattgaaaa
     1321 aacaaattat gattatgaaa taatttttgt aaatgatgct tcaacagata attcccttga
     1381 aattttaaaa gaatatgcca ttgaagacag aagaataaaa ataataaacc tctcaagaag
     1441 atttggatat aatcaaggac ttattgcagg acttaattat tcaaaaggag acgcaattat
     1501 aacacttgat gctgatttac aggaccctcc agaacttatt ccagaattaa taaaaaagtg
     1561 ggaaaatggt gcagatatag ttcatacaat tagaaaaaaa agagaaggag atagtaaaat
     1621 aaaagttatt cttatcaaaa tcgcatataa aattataaac tttgtatctg aaattagatt
     1681 accccaagat gctggaaatt ttaagttaat atcaaggaaa gcattaaatg aagttttaaa
     1741 attgaaagaa aaagaccctt atattagagg acttgttgta tgggctggtt ttaaacagga
     1801 ctatatttat tatatcagag aaaaaagggc aaaaggtaaa actcattttc catttttaag
     1861 aagtatggga ccttttatgg aatttataag tggagtaact tctttttcta ttttgccttt
     1921 actttatata gttgttcttg gacttatttt aatttttctt ggaattgttt tttttatact
     1981 ctcaattttt catcttattt atttctggat ttcttttatg gttttctttt caggtattat
     2041 tattttctca aatggaattc tgggtgttta tatcggaaga atatggaagg aagtattaaa
     2101 caggccaaat tatatagttg aaagtactat caatattgaa gagaataaat attgacttta
     2161 tttaaagatt gaaattttat catgaagaaa aacgagatgg ttataatatt ttaaaaggaa
     2221 gtttgaaatt aaagaagaat caatcctctc agacatacca agagaaataa ttctttgcgg
     2281 gtcagagtat atattaaatg caattgtaaa aacagttaag ataaaaagtg tttctattag
     2341 aaaaactgaa ctaaaaataa aaaaattttt ttcaggcata gaaagtatat actttattcc
     2401 aaaacagata aataaacatg taagtggaag caatcctgta tgagcaagtc ctgacaattc
     2461 ttttaaaggc ataacaacta tatctaaaag ataactcaaa aaaaagaaaa aaataagaaa
     2521 agaatgagaa gaaagtgttt ttaaattaat ttgtttaatg ttttttaaaa ataaaattaa
     2581 caaaaagaaa gttaatgaaa atgtcaaact tcctggtagt gtacatacat aagcaggatg
     2641 aaggtaatta aaaaatttgt aaaataaagg tgaattattg aaaaaatatt tgtagtattt
     2701 taatggaaaa ggatgtaagg tttttataaa attaaaggaa tctttttcaa ttctatcaat
     2761 caaacttata tttttctgct gttgatatat atttgttgag aaaaaagatt tataaactcc
     2821 atagttttta attgaccatg aaaaatatgt aaaaatcaca aataagaaaa tcaaataaga
     2881 aatccctgtt ttttttatca aatttttatt aagaattgtt ctaaaagcaa gatgtaaaaa
     2941 aacaggaact atataacaac cagcagaaaa atgaactata aaacctatac cgaataaaaa
     3001 acttgaaata aataaatgaa gagaattctg atttttaaga aatttaaggt aaaaataaag
     3061 tcctaaaata acataaaaag cacaaaatgc ttttgtccag gtataagtta tctgtcttaa
     3121 aatcgctgga tttaaaaaca taacaacaca aactattaaa aataagtatt tattttcaat
     3181 tttaaggtat tctttacaaa aaagataaac tgataaaatt aacattgagt ttaaaaaagt
     3241 tgaaacaatc tgatatttat aaaattctcc acctgtaatt gaaagataga aaaaacaaac
     3301 aatattgaaa aaaggaggtc ttgaaggaat aatatacccc tcaaaaacta tgtcttttgg
     3361 taatctatca agaaaaaaaa tacttcttaa aaaatgttca aaccagtccc agtaaaataa
     3421 accaccaaaa taaagaggaa taagtgtctg gtaaagaata aaaactaaaa aaattataaa
     3481 aaaatttttt aaaaatttaa tttcctgatt atcaaaacca tttttaagaa tataaaagaa
     3541 agaaaaaaat aagaagatta aaagaaaaat accattaaaa atcgcaaaat tagagttaaa
     3601 aacataactt aaaaaaccta ctatgtaaaa aagaaaaaaa ctgaaagcaa aattcagaat
     3661 aaatttttct tctttttcaa aaggtaaatt ttgagtaaga aatcttgcag gaaaataacc
     3721 actaaaaaaa atgaaaaaac ttaatatcaa aatttcaaat acatttttca tcagacttat
     3781 tttataacta ttcaaaaaaa tattcaaaag tttacaaaaa tcaacagaaa tgataacatt
     3841 taaatatata aagaggatag aaaaatgaaa atacttgtta ctggtggagg tggttatata
     3901 ggttcggtat tggttcctat tttattacag aaaggttatg aagtaattgt tcttgataat
     3961 tttatgtata accagacatc tcttcttgat tgctgtcatt taaaaaacct tgaaataata
     4021 agaggagaca caagagatga aaatttatta aaagaaatat taaaagaagt tgatgttata
     4081 tttccacttg cctgcataac aggggctcct gcctgtgata gaaatcctta cgaagcaaaa
     4141 tctgtaaatc ttgacgctat aaaattgata cttaaattga gaagtaaaga acaaataatc
     4201 atatttccaa caacgaatag tggttatgga attggacagg aaggaatata ctgcacagaa
     4261 gaaactcctt taagaccaat atctctttac ggaagattaa aagttgaagc agaaaaagca
     4321 gttctggact caggaaacag tatcacttta agatttgcaa catgttttgg aataagtccg
     4381 agaatgcgac ttgatttact tgtcaatgat tttgtttaca gagcggttgt agatagatat
     4441 cttgttgttt atcagggaca ttttaaaaga aattatatac atgtaaggga tgcttctttt
     4501 tctttcattc actgtcttga gaattttgaa aagatgaaaa ataatactta taatgttgga
     4561 cttagtgatg caaatataag caaattagaa ctgtgtgaac tgataaaaaa acatatccct
     4621 gatttttatt atcatgaagc agaggttgga gaggaccctg ataaaaggaa ctacattgta
     4681 agtaatgaaa agattgaaaa aaccggatat aaagcaaaaa tttcacttga cgaaggaata
     4741 aacgaattaa taaaaggtta taaaattata agaaaatatg gcttttcaaa tgtttaaaat
     4801 aaaaaatgat tgacctttta cttgtaaatc caggaaatcc taaaaaagtt tatgggaaat
     4861 tgagaagtaa tcttgccgga atagaaccac catactggtg tgctttaatt gcttctttta
     4921 taagaaaaaa aggttattca gttaaaataa tagatgctga tgctgaaaat ttatctcctg
     4981 aggaaactgt aaatgaaata atcaaaaata atccggttct tgttaatatc gttgtaatgg
     5041 gaacaaatcc ttctgcttct tcttctccca aaatgtcaac cgttagagaa ttacttaac
//
LOCUS       DAOSAT010000109         5086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_10322_length_5086_cov_5.807195, whole genome shotgun sequence.
ACCESSION   DAOSAT010000109 DAOSAT010000000
VERSION     DAOSAT010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10322_length_5086_cov_5.807195"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLF90_08295"
     CDS             <1..218
                     /locus_tag="PLF90_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57311.1"
                     /translation="PEYKSFFWDCQNEVILEKFILRVLNYGNIEDIEEIYKKYPEETY
                     DIAFRYPEIKRGIKFWLKLWKEEKLKK"
     gene            191..775
                     /locus_tag="PLF90_08300"
     CDS             191..775
                     /locus_tag="PLF90_08300"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOK57312.1"
                     /translation="MERGKIEKMKKLAVEIQKIFKNFYLAGGTVLMFKYNHRISEDLD
                     FLSEKEFSFLRLSSKVRKIFNPEKEERFVDNIDFIIDGIKTSFVFFPFKNINLIEKYE
                     KIKIVSDYDIFLNKIYCSGRRIETKDLIDFAFLYEKYGWEKEKVKKDFEKKFPLQNFE
                     IYLGAILSVEDYPDADTKTLKIIKEVKKRWGIES"
     gene            780..1091
                     /locus_tag="PLF90_08305"
     CDS             780..1091
                     /locus_tag="PLF90_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57313.1"
                     /translation="MENTYIKKKLKNSLSSYNLDKISFKRDKRLIIENVLNHGGDEEI
                     LWVLKNYKKKDIVSVLKNPSRGFWNKKSLNFWLAIFGVKIGKDKYERAIREIFIKSDT
                     H"
     gene            1045..1701
                     /locus_tag="PLF90_08310"
     CDS             1045..1701
                     /locus_tag="PLF90_08310"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOK57314.1"
                     /translation="MKEQLEKSLLNPIHTEILSKEQKEILKELKFTKKMNFYLADGTG
                     LALYLGHRTSIDFDFYSQQQFKTLSVYFKNGEIVNDTEDTFEIIVKNTHLSFFYYPYK
                     LIRKPVEMENILVASIEDISAMKIISIVQRGNYRDFIDIYFLMKIFGIKKLINWLKEK
                     YLNYSTFLILKGLIYFEDADKTVKEDKERIRMIEKIEWKQVKKFIKKEVEKFVKNYSI
                     "
     gene            1985..2057
                     /locus_tag="PLF90_08315"
     tRNA            1985..2057
                     /locus_tag="PLF90_08315"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2018..2020,aa:Ala,seq:ggc)
     gene            complement(2190..3125)
                     /locus_tag="PLF90_08320"
     CDS             complement(2190..3125)
                     /locus_tag="PLF90_08320"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOK57315.1"
                     /translation="MLVIKIFSVFLIIIAGIIVRKKGLISSLTIKEMSVIITKVFYPS
                     LIFSSFLRNFTSEDIINNYILPLGTILIMFVGYLYGIIFTKFIRFNNEKEKNTFHFQC
                     TINNYSFLPLPIILFLYGEKDIPKLILSTFGSEISVWTIGILSLTGNRFEKKALKNLL
                     SIPMISIIFSVILIFLENVITIENQVIIESGKSILNVITLLGNATIPVALFIAGAKMG
                     EIELKDIFIFKSLFLAILRLILIPLTCIFLFYIFSFPYEIRRVLIVVTIMPCAIASIV
                     LSEAFEGDSKFAAETVLLTHLFSLITIPLFLYFFK"
     gene            complement(3130..4209)
                     /locus_tag="PLF90_08325"
     CDS             complement(3130..4209)
                     /locus_tag="PLF90_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57316.1"
                     /translation="MEKIACLISFLISSAGYVINLSIPLYLISKFSASPFILGIAGFF
                     GNFAYTTFTYIFHKFRWRLHFPWFIISSLFISLNYFFLPFCPHYKYMFILFFINGIFY
                     SRFWPSIQYIFSQNSIHIDRYNISWSSGVIFGIFISGYLFKVKDTLPFWMGCFFAFIG
                     FLSGFINFKKFKLFYRDLPEKFYERKPLDKETKKVMLLNFSSFFAIGGFLFVFPKLAN
                     NIGYSSNLISNIITFLFLIRTSMFYVFSKIKIRTTDKILFFNYLFICLSLIGAGISKN
                     FPLHLIFISLLGFCSAYSYRLGLFTVIEKGFSTELNESIIGIGLFTGPLIVGILSQIF
                     GIFNGFIFSGVLILIIFLFQKYLIK"
     gene            4345..>5086
                     /locus_tag="PLF90_08330"
     CDS             4345..>5086
                     /locus_tag="PLF90_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOK57317.1"
                     /translation="MNVRKLLREISERKGNDVAFVFKDVDYTYKTLEENTNKVANFLK
                     ELGVKKGTKVGIYMLNNPEYIYSYLAIFKIGAIAVPLDHRLKTEEIFPLLNHCEAEFL
                     ITTISKEFNPDLIMKNVPSLKNIIITKGTFQNCVSLEEKIENFSPEFSGNEINDSDIA
                     AIFYTSGTTGVPKGVIWTYKHLESPIDTIFHHYHYCEENDVCISPIPFSHNGGIVAIL
                     LILFGVKLVVMEYYHPLELLQNLQKYNVA"
BASE COUNT         2072 a          583 c          762 g         1669 t
ORIGIN      
        1 cacctgaata caaatctttt ttctgggact gtcagaatga ggttattctt gaaaagttta
       61 ttttgagggt tttaaattat ggaaatattg aagatataga agaaatttac aaaaaatatc
      121 ctgaagagac atatgatatt gctttcagat accctgaaat aaaaagaggg ataaaattct
      181 ggctaaaact atggaaagag gaaaaattga aaaaatgaaa aaacttgctg ttgaaatcca
      241 aaaaatcttt aaaaattttt atcttgctgg tggaacagtt ttaatgttta aatataatca
      301 tagaataagt gaagaccttg atttcctttc agaaaaagag ttttcatttt tgagattatc
      361 atcaaaagtc agaaaaatct ttaatccaga aaaagaagaa agatttgttg ataatattga
      421 tttcataata gatggtatta aaacttcatt tgtctttttc ccatttaaaa atataaatct
      481 aattgaaaaa tatgaaaaga taaaaattgt atctgattat gatatatttc tcaataagat
      541 ttattgctct ggaaggagaa ttgagacgaa ggatttaatt gattttgcat ttttatatga
      601 gaaatatgga tgggaaaaag aaaaagttaa aaaagatttt gaaaagaaat tccctttaca
      661 aaattttgaa atatatttgg gtgcaatact ttcagtagaa gattatcccg atgctgatac
      721 taaaactttg aaaataataa aggaagtaaa aaaaagatgg gggatagaaa gttaaattta
      781 tggaaaatac ttatattaaa aagaaattaa aaaattcgct ctcaagctat aatcttgata
      841 aaatttcttt taagagagac aaaagattaa ttattgaaaa tgttttaaat cacggaggag
      901 atgaagaaat actttgggtt ttgaagaatt ataaaaaaaa ggatattgtc tctgttttaa
      961 aaaatccatc aagagggttc tggaataaaa aatcattaaa tttctggtta gcaatatttg
     1021 gggtaaaaat cggaaaagat aaatatgaaa gagcaattag agaaatcttt attaaatccg
     1081 atacacactg aaattttaag taaggagcaa aaagaaattt taaaggaatt aaaatttaca
     1141 aaaaagatga atttttatct tgctgatgga acagggcttg ctttatatct tggccataga
     1201 acatctattg attttgactt ctattcacag caacaattta aaacactttc tgtttatttt
     1261 aagaatggtg agatagttaa tgacacagaa gatacatttg aaataattgt taaaaataca
     1321 catttatctt ttttttatta cccttacaaa cttataagaa aaccagtgga aatggaaaat
     1381 atacttgttg catcaataga agatatatct gcaatgaaaa taatttctat tgttcagagg
     1441 ggaaattaca gggattttat tgatatatat tttttaatga aaatttttgg aataaaaaaa
     1501 cttattaatt ggcttaaaga aaaatattta aactattcaa cttttttgat tttgaaagga
     1561 cttatttatt ttgaagatgc tgacaaaact gttaaagagg ataaagaaag aataagaatg
     1621 attgaaaaaa tagaatggaa acaagtaaag aagtttatta aaaaagaggt agaaaaattt
     1681 gttaaaaatt attcaatatg ataaaatata aataggggaa aaaatgattt tgttttggaa
     1741 ataataaaag ataaaattgg gtttcaatct gccaaagagg aatttaaacc tagaattaca
     1801 agatttagca aaagatttct ttactcagag tttcaatctg ccaaagagga atttaatttc
     1861 ccacctttaa ggtgggcttg gagaaagaag gaaagacaag ggatttagag gcctattttt
     1921 taatgaggca attttaaatg ttgacttttt gaagggatac ccttataatt tatttattga
     1981 aaatggggga ttagctcagt tgggagagca ccaggctggc agtctggggg tcaagggttc
     2041 aaatccctta tcctccattg gtttttctct ttgatgcttg ggtaatacta acgcagaaaa
     2101 gataaacata aaaactctga aaatactaaa tttatctgaa ttaatgaaac ctaaaaaact
     2161 tttattatat atttttcatt taaaatttct cacttaaaaa aatagaggaa aaggggaata
     2221 gttataagag aaaaaagatg tgttaaaagg actgtttctg ctgcaaattt actatcacct
     2281 tcaaatgctt cacttaaaac aatacttgca attgcgcatg gcataatagt tacaacaatt
     2341 aaaactcttc ttatttcata ggggaaagaa aaaatataga ataaaaagat acaggttaaa
     2401 ggaattaata tcaatcttaa aattgcaaga aacaatgatt taaatataaa aatgtccttc
     2461 aattcaattt ctcccatttt tgcacctgca ataaataaag caacaggtat ggtagcattc
     2521 cccagaaggg ttataacatt taaaattgat tttccacttt caataattac ttgattttct
     2581 atagttatta cattttctaa aaatatcaat ataacagaaa aaattattga aatcataggg
     2641 atacttaata aattctttaa tgcttttttt tcaaatctgt ttcctgttaa tgatagaatt
     2701 ccaattgtcc agacagaaat ttcagaacca aatgttgaaa gtataagttt tggtatatct
     2761 ttttctccat ataaaaaaag gataatgggt aagggcaaaa atgagtaatt atttattgta
     2821 cattgaaagt gaaaggtgtt tttttctttt tcattattga atctaataaa ttttgtaaaa
     2881 attattccgt ataggtaacc tacaaacata ataagaattg ttccaagtgg cagaatataa
     2941 ttatttataa tgtcttctga tgtaaaattt cttaaaaatg aagagaatat aagagaggga
     3001 tagaaaactt ttgttataat tacagacatt tctttgattg tcaaagatga aataagtcct
     3061 ttttttctga caattattcc tgcgataatt attaaaaaaa ccgaaaaaat tttaattaca
     3121 agcatttttt tattttatta gatatttttg aaaaagaaaa attattaaaa taagaacgcc
     3181 tgaaaaaata aatccattaa atattccaaa aatttgactt aaaataccaa caataagagg
     3241 acctgtaaat aaacctattc caattattga ttcattgagt tctgttgaaa atcctttttc
     3301 aataaccgta aaaagaccca atctatagga atatgcggaa caaaaaccaa gaagtgagat
     3361 aaatattaaa tgaagaggaa aattttttga aatacctgct ccaataagtg aaagacatat
     3421 aaaaagatag ttaaaaaata atattttatc agttgtcctt atcttaattt tgctgaagac
     3481 ataaaacatg gatgttctga ttaaaaaaag gaaagttatg atattactta taagatttga
     3541 agaatatcct atgttatttg caagtttagg gaaaacaaat aaaaaacctc caatcgcaaa
     3601 aaaacttgaa aaatttaaaa gcattacttt tttcgtttct ttatcaagag gttttctctc
     3661 ataaaatttc tcaggtaaat ccctgtaaaa taatttaaat tttttgaaat ttataaatcc
     3721 agataaaaaa ccgatgaatg caaaaaaaca acccatccag aatggtagag tatcttttac
     3781 tttaaaaaga tatcctgaaa taaaaatgcc aaaaattact ccgcttgacc atgaaatatt
     3841 gtatctatct atatgaattg aattctgact gaatatgtat tgaattgaag gccagaatct
     3901 gctgtaaaaa atcccgttta tgaaaaacag tatgaacatg tatttatagt gtggacaaaa
     3961 aggaaggaaa aaataattta aagaaataaa tagagaagaa attataaacc acggaaaatg
     4021 taatctccat ctaaacttat gaaaaatata tgtgaaagtt gtataagcaa aatttccgaa
     4081 aaaaccagca ataccaagaa taaatggaga agcagaaaat tttgaaatta aatataaagg
     4141 aattgaaaga ttgataacat aaccagcaga cgaaattaaa aaagaaatta aacaagcaat
     4201 tttttccata tttattaccc ttaaactcaa gatattcaat tttaaatttt tattatagaa
     4261 tttttatttc accaatacaa gaaagtaaaa aaactttgtt tttcaaaaaa aatattttaa
     4321 aataaattgt aaaaaggagg gaatatgaat gtaagaaaat tgttaagaga aattagtgaa
     4381 agaaaaggta atgatgtggc ttttgtattt aaagatgtgg attatactta taaaactctt
     4441 gaagaaaaca caaataaagt tgcaaatttc ttaaaagaac ttggagttaa aaagggtaca
     4501 aaagttggaa tttatatgct taacaatcct gaatatattt attcttatct ggctattttt
     4561 aaaattggag caatagcagt tcctcttgac cacagattga aaacagaaga aatttttcca
     4621 cttcttaacc attgtgaagc agaattttta atcaccacaa tttcaaaaga gtttaatcct
     4681 gaccttataa tgaaaaatgt gccgtctctt aaaaatatta taataacaaa aggcactttc
     4741 cagaattgtg tctctcttga agaaaagatt gaaaattttt ctcctgaatt ttctggaaat
     4801 gaaataaatg actctgatat tgcagcaatt ttctatactt cagggacaac aggtgttcca
     4861 aaaggtgtta tatggactta taaacatctt gaaagtccaa ttgatacaat ttttcatcat
     4921 taccattatt gtgaagaaaa tgatgtatgt atatctccaa tccctttttc ccacaatggt
     4981 ggtattgttg ctatccttct tattcttttt ggtgttaaac ttgttgtgat ggaatattat
     5041 catcctcttg aactcctcca aaacctgcag aaatacaatg ttgcag
//
LOCUS       DAOSAT010000110         4828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_11074_length_4828_cov_4.979468, whole genome shotgun sequence.
ACCESSION   DAOSAT010000110 DAOSAT010000000
VERSION     DAOSAT010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11074_length_4828_cov_4.979468"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PLF90_08335"
     CDS             complement(<1..940)
                     /locus_tag="PLF90_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOK57318.1"
                     /translation="MAKVTFIGAGSFGFTRTLVRDLLTFPLLKDATISLMDIDAERLE
                     FSKRAVEKIIAEGKYPAKLEVTMDRREALKGADAVLCTILSGGVNIWRYDIEIPKKYG
                     VDICVGDTRGPAGIFRALRTIPVMLDICRDIEEICPDTIFLNYTNPMSMLCRAMQKKT
                     KVKVTGLCHSVQGTAEMLAKWIGATMDQITYVCAGINHQAWYIEYKKDGKDAYPLIKK
                     VILENEEIYKEEIVRNEMFLALGYYVTESSGHNSEYNWWFRKRQDLIGKYCLPGTGWN
                     PGEYAYILKEYLKREDTWKDEIKKWLEKPVNLERGHE"
     gene            1233..1715
                     /locus_tag="PLF90_08340"
     CDS             1233..1715
                     /locus_tag="PLF90_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57319.1"
                     /translation="MEKTIKVINELKKKGLINDYAIGGGIGALFYIEPFLTYDLDIFV
                     VLKEEPDKVIIDLSNIYNYLQSKGYKWKGEHILIEGVPVQFIVADELEEEGIKNAKKV
                     RYKGIPTKVLSPEYLIAIFLRTGRKKDIEKVERFFEETKINKRKLRNILKKFNIKWVK
                     "
     gene            1712..1903
                     /locus_tag="PLF90_08345"
     CDS             1712..1903
                     /locus_tag="PLF90_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57320.1"
                     /translation="MSKEIKICINGKVIKREDIFKRKDIFHKEQAKLPFEKKIEILVT
                     LQKIARNVKGKKGIVWKIE"
     gene            complement(2232..2537)
                     /locus_tag="PLF90_08350"
     CDS             complement(2232..2537)
                     /locus_tag="PLF90_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOK57321.1"
                     /translation="MKEKIDYREVLKETVEALENGLVLLVSVDKKGKPNPMAIGWGAM
                     GEIWGKPVFIVLVRPSRYTYSLIEQTEDFSVNVADESMKDIVIYCGTVSGRNHDKFK"
     gene            complement(2521..3579)
                     /locus_tag="PLF90_08355"
     CDS             complement(2521..3579)
                     /locus_tag="PLF90_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOK57322.1"
                     /translation="MGVAIWQFITAFLFILLGFAGKKISEYIIETKIIKITKRTRFEL
                     DDLIVNAFSRPFSFGIALLGFSLAIAVLPVSQTAERIIYSIIKIAGVVIFLWLLFRLV
                     DVIVVYLSRLTERTESKLDDQLVPLINKALKVTIGIICFLWLLQLLGYNVSSLIAGLG
                     IGGLAVALALQNTLSNFFGSIFIFLDRPFMVGDWVKIGDVEGIVEDIGFRSTRIRTWP
                     ATLVSIPNKKVAESVIDNWSRMPKKRVYQTIGLTYETTADQMENAVREIRKIIENDPG
                     VDKEFIVVKFSDFGSSSLDITVIYFTIDTTLKGHLETKERINLAIMRKLKELGLSIAF
                     PTMSIYLEKGEIKIERKD"
     gene            complement(3714..4493)
                     /locus_tag="PLF90_08360"
     CDS             complement(3714..4493)
                     /locus_tag="PLF90_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOK57323.1"
                     /translation="MEKPIIFESEGKNLIGILHLSEFKNSPVVVLFHGFTGQKSESHF
                     IFTRLARLLCKEKISVLRFDFMGSGDSEGEFSDMTLYTEMKDGENALRYIKELENVNK
                     EKIGILGLSMGAVTASYIASEFNTTSLCLWSPVAYPSVISRRLTRKIKKQLQEKGKAY
                     LPGTGLYISDEFIKSTKEVKPLRFAGKYKGSVLIIHCKDDTVLPVEHALAYFKKFHQQ
                     SRFSQLTIFEKGGHTFTVEETEKEVLNQTVNFFKTTLWGGK"
BASE COUNT         1602 a          866 c          720 g         1640 t
ORIGIN      
        1 attcatgacc tctttcaaga ttaacaggtt tttcaagcca tttttttatt tcatctttcc
       61 atgtgtcctc tcttttaaga tattctttaa gtatataagc atattctcca ggattccagc
      121 cagttccagg aagacaatat tttcctatca aatcctgcct ttttctgaac caccagttat
      181 attcagaatt atgtccgctt gattcagtaa cataatatcc aagagcaaga aacatttcat
      241 tccttacaat ttcctctttg taaatttctt cattttccag tataactttt ttgataagtg
      301 gatatgcatc tttgccatct tttttgtatt caatatacca tgcctgatga ttaattcctg
      361 cacagacata agtaatctgg tccatggttg ctccaatcca tttcgcaagc atttctgctg
      421 ttccctgaac actgtgacat aaacctgtaa ctttgacttt tgtttttttc tgcattgccc
      481 tgcacagcat tgacattgga tttgtataat taaggaagat tgtatcagga cagatttctt
      541 caatatccct gcatatatca agcataacag gaatagtcct taaagcacga aaaattccgg
      601 caggacctct tgtatctcca acacaaatat ctactccata tttttttgga atttctatat
      661 catacctcca tatattaact ccacctgaaa gaatagtaca taaaacagca tctgctcctt
      721 taagtgcttc tcttctatcc attgttactt caagttttgc tggatattta ccctctgcta
      781 taattttctc aactgctctt ttagaaaatt caagtcgttc agcatctata tccattaaag
      841 aaatagtagc atcttttaac aatggaaatg taagtaaatc tcttacaaga gttcgtgtaa
      901 aaccaaaact acctgctcca ataaaagtaa cctttgccat tttttcttcc aattatttaa
      961 ttttaatttg tctattttaa cccaataatt ttttctgtgc aagtcaatat aatattataa
     1021 aaatttttat ccaaaccatc ttacttatcc caccttaaag gtgggcaatg gagatgattg
     1081 atacaagcgg gttttaaagg tcgggtttaa gaggtgaatg tttttcaata ggaataaaag
     1141 ttaatgagtt tttaatattt tgaccggttg atttataaac tttataattt aacagaagaa
     1201 gagatttgat ttatagagcg gaaaacgaga agatggaaaa aactattaaa gttataaatg
     1261 aacttaaaaa gaaaggattg attaatgatt atgcgattgg aggtggaatt ggagcccttt
     1321 tttatattga acccttttta acttatgacc ttgacatttt tgttgtctta aaggaagaac
     1381 ctgataaagt aataattgac ctttccaata tttataatta cttgcaatca aaaggatata
     1441 aatggaaagg tgagcatatt ttaattgagg gggtgccagt tcaatttata gttgctgatg
     1501 aacttgaaga agaaggaatt aaaaacgcca aaaaggtaag atacaaagga atccctacta
     1561 aagtgctttc tcctgaatat ttaatagcaa tatttctgag aaccggacgc aaaaaggata
     1621 ttgaaaaggt ggaaagattt tttgaagaaa caaaaattaa taagagaaag ttgagaaata
     1681 ttcttaaaaa gtttaatatt aaatgggtaa aatgagtaaa gaaataaaaa tttgtataaa
     1741 tggaaaagtt ataaaaaggg aagatatttt taaaagaaag gatatttttc ataaagaaca
     1801 ggcaaaactt ccttttgaaa aaaagataga aattcttgtc acacttcaaa aaatcgcaag
     1861 aaatgtcaaa ggaaaaaaag gtattgtatg gaagattgaa taaaacaaaa tttccctatc
     1921 ccaccttaaa ggtgggcaat ggagatgatt gatacaagcg ggttttgaag gcacatactg
     1981 atttgacagt caagttagca gttaatattt aaacaaagtg atttttagcg aagtgttttt
     2041 aaacaagaaa gaatttctcc ataataaact ctgtgaaagt catcctgctt gtaattagag
     2101 ttttttattt cttcggaaat aaattctgaa attatatctg tctttgcaat aactctacat
     2161 tcaaagttta ttctacattc ctcaattatg ggggatttta taaactctga ttttaatgga
     2221 gctaaatttt tctatttaaa cttatcatgg ttcctaccag aaacagttcc acaataaata
     2281 acaatatctt tcatggattc atctgcaaca ttcacactga aatcttctgt ctgctcaatg
     2341 agtgaatagg tatatctgga aggtctgaca agaactataa aaactggttt tccccagatt
     2401 tcacccattg ctccccatcc aatagccatt ggatttggtt ttcctttttt atctacagaa
     2461 acaagcaaaa ccagcccatt ttcaagggct tctactgttt cttttaaaac ctctctataa
     2521 tcaatctttt ctttcaattt taatttcccc tttttctaaa taaatactca ttgtcgggaa
     2581 tgcaattgaa agtccaagtt ctttaagttt tctcattatt gcaagattta tcctttcttt
     2641 tgtttcaagg tgtcctttca atgttgtatc aattgtaaaa tagataaccg taatatcaag
     2701 agaggaagaa ccaaaatcac tgaattttac cacaatgaat tccttatcaa caccggggtc
     2761 attttcaatt atcttcctta tttccctgac agcattttcc atctggtcgg ctgttgtctc
     2821 ataggtaagt ccaatcgtct gatagactct ttttttaggc atccttgacc agttatctat
     2881 tactgattct gcaacttttt tatttggtat ggaaacaagt gtggccggcc aggttcttat
     2941 tcttgtagac ctgaaaccaa tatcttcaac aattccttca acatctccta ttttaaccca
     3001 atcacctacc ataaacggtc tatcaagaaa aataaaaatt gagccaaaaa aattactaag
     3061 ggtattctgt aaagcaagag caacagcaag gccacctatt ccaagacctg caattaaact
     3121 tgaaacatta tatccgagca attgtaataa ccacagaaaa caaattattc caattgtaac
     3181 cttcagtgct ttattgataa gtggtacaag ttggtcatca agttttgatt cagttctctc
     3241 tgtcagacgg gaaaggtaaa caacaataac atcaacaaga cggaaaagga gccatagaaa
     3301 tattacaaca cccgcaattt ttataattga ataaattatt ctttctgctg tctgagaaac
     3361 cggtaaaacc gctattgcga gagaaaaccc aagcaacgct attccaaaac tgaatgggcg
     3421 ggaaaaagca ttaacaatta aatcatccag ttcaaacctt gttctttttg taatttttat
     3481 aatttttgtt tcgataatgt attcagaaat ttttttacca gcaaacccga gaaggataaa
     3541 aagaaatgca gttataaact gccatattgc cactcccata aacgttttac tcatccaaga
     3601 tggaaaatga gaagaccaat taattttggg aagcaggtgt gtttctacta ttgaagtatc
     3661 tgaggaagcg gaagtaaaca atgtattcaa aaaaacgaag ttaaaccatt ttctcacttt
     3721 ccaccccaca gagttgtttt gaaaaaatta accgtttgat tcagaacttc tttttctgtt
     3781 tcttcaacag taaaagtgtg accacctttt tcaaagattg taagttgaga aaatcttgac
     3841 tgctggtgga attttttaaa ataagccaga gcatgttcaa caggtaaaac cgtatcatct
     3901 ttacagtgaa ttataagcac acttccttta tattttcccg caaatcggag aggtttaacc
     3961 tcttttgtgc ttttaataaa ttcatcactt atataaagtc ctgttcccgg aagatatgcc
     4021 ttaccttttt cctgaagttg tttttttatt ttccttgtta aacgccttga aataacagaa
     4081 ggataagcaa ccggcgacca gaggcagaga gaagtggtat taaattcaga agcgatatag
     4141 gaagcggtta cagcccccat agaaagaccg agaattccaa ttttttcttt attcacattt
     4201 tctaactctt taatatatct cagtgcattt tctccatctt tcatttcggt gtacagtgtc
     4261 atatctgaaa attccccttc actatcaccc gaacccataa agtcaaatct taaaactgat
     4321 attttttctt tacagagcaa tctggcaagt cgtgtaaata taaaatgact ttcacttttc
     4381 tgacctgtaa aaccgtggaa taagacaaca accggtgaat ttttaaattc tgataaatga
     4441 agtattccga ttaaattttt accctcactt tcaaaaatta tcggtttttc catttttttt
     4501 tcagagttca aattctttta tcttttttat tatatcttta acttcaactc tgtcaaaaca
     4561 ttcctttact ttacacaaat tcattttttc ctgactgcac ggattacaac ctgaattttt
     4621 aaaaaatact ttgatatttt ggccctgtgt acagataaaa aacgatgtgg gtctttatcc
     4681 tcccaatttt ttctttccac cattcatatg atcaaactct gcttgtttaa aaataactct
     4741 ggctttttca tatctaaaat tgtttaaaag agcaaaaagc tccggtggta ggattcgaac
     4801 ctacaacctt gcggttaaca gccgcccc
//
LOCUS       DAOSAT010000111         4820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_11103_length_4820_cov_4.700525, whole genome shotgun sequence.
ACCESSION   DAOSAT010000111 DAOSAT010000000
VERSION     DAOSAT010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11103_length_4820_cov_4.700525"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="PLF90_08365"
     CDS             <1..1257
                     /locus_tag="PLF90_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOK57324.1"
                     /translation="RNSKNVDEAREKLIKTFKITEIQANAILSMPLSRLTGLEREKIL
                     KERDEIELKIKDYKEILESPKRVKEIIKNELLLIKEKFGDDRKTEIVESIEEFEDIDL
                     IRPEDIVVTISYEGYIKRTNLDSFRRQRRGGKGLIGASTKEEDFIKHLLLTSTTDTLL
                     FFSDKGKVYWLKGYLIPEGSRQAKGKPIINLLKIEPGEKISAVIPVREFSKDKFLIMI
                     TKNGLVKKTSLEKYSKPRKGGIIGIDLREGDKLIDVKLTDGNSHIILSTKNGLSIRFP
                     EKEVKPVGRNAIGVIGIRFKKKDDEVVGCEIINPEEENFSLLTVCKKGFGKRTLIKEY
                     KVQHRGGKGVIDIKVGNKNGEVVGSKLVSGEDEIIITTKKGIVIRIKVSDIRIVGRNT
                     IGVKLINLPSDDEITDITKVEKENNE"
     gene            1250..1663
                     /locus_tag="PLF90_08370"
     CDS             1250..1663
                     /locus_tag="PLF90_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOK57325.1"
                     /translation="MNKNIEIINHTADTGIIVKGKTIEEIFINSLKGLYEIMGVKSNG
                     KESIMEINLKSEELENLLVKFLNEMIYYMETKKMSGEIKEIKIDFKNNEYNLFAKLKM
                     QKVEKIEKEVKATTYHNLKIKKLDNQYTTTIIFDL"
     gene            1678..2025
                     /locus_tag="PLF90_08375"
     CDS             1678..2025
                     /locus_tag="PLF90_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOK57326.1"
                     /translation="MKKEEKNYFLKLLEREKEKIFKNLSYAREVIEKGEKGVPTHIAD
                     YGTDEFEKDLEVGLSNSESKLLEEINIAIKKIKDGTYGKCDNCGQLISKERLKAIPYA
                     RYCIKCQTEREKK"
     gene            2022..2477
                     /gene="lspA"
                     /locus_tag="PLF90_08380"
     CDS             2022..2477
                     /gene="lspA"
                     /locus_tag="PLF90_08380"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOK57327.1"
                     /translation="MKFRFFLKIFLTTSFLALFFDQITKSFIVRLLKKTDGNINITSF
                     LSFTLIKNQGIVFGLFNNEKTRIFIIIFSFIAIFFIIFAILKLKKTEKYHQFSLGLIV
                     GGTIGNLLDRIRFGYVIDFINFHFWPVFNFADAFISIGVILFFIKYLKE"
     gene            2480..3223
                     /gene="lgt"
                     /locus_tag="PLF90_08385"
     CDS             2480..3223
                     /gene="lgt"
                     /locus_tag="PLF90_08385"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOK57328.1"
                     /translation="MHPVFIKIGNFVIYWYGVFVAVGVFISIALFEKEYHKEGYDEKF
                     ISQLVFLTIFTGIIGARFLHILVNIYYYILHPLEIIKIRNGGLAVEGAILSSLIFIIL
                     FSKIKNFSPVKLLDTIAIYVPIGQAIGRLGCFLNGCCYGKETDFFIGVKFPFMDKKVH
                     PTQLYYSFLYIVLFFFLKKLSKKTKKGEGFIFSSYLIGFSLIRYFVDFLRGDLKRTSL
                     GLYSTQFIAILIFMIGGIYIIVKLIKGRK"
     gene            3224..3589
                     /locus_tag="PLF90_08390"
     CDS             3224..3589
                     /locus_tag="PLF90_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOK57329.1"
                     /translation="MAKLVFDYPFMNREYELVKEKIYVGRQNTNDITIPDYQIFQSLP
                     ESTKRLYLDSLVKVSRIHARIIKKDDGWYIEDIGTHGLGSQYGTYVNMARIEVKKPYK
                     LQENDEIQFGPIKCKFFEK"
     gene            3649..4820
                     /locus_tag="PLF90_08395"
                     /pseudo
     CDS             3649..4820
                     /locus_tag="PLF90_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609562.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
BASE COUNT         2005 a          484 c          825 g         1506 t
ORIGIN      
        1 agaaattcca aaaatgttga tgaggcaaga gaaaaattaa ttaaaacatt taaaattact
       61 gaaatccagg caaatgcaat tttaagtatg cctctttcaa ggttaacagg acttgaaagg
      121 gaaaaaatat taaaagaaag agatgaaatt gaattaaaaa taaaagatta taaagaaatt
      181 cttgaaagtc caaaaagagt aaaagaaata ataaaaaatg aacttttatt gatcaaagaa
      241 aaatttggag atgatagaaa aacagaaatt gtagaaagta tagaagaatt tgaagatatt
      301 gatctgataa gacccgaaga catagttgta acaatttctt atgaaggtta tataaaaagg
      361 acaaaccttg attctttcag aagacagaga agaggaggca agggattaat aggagcttca
      421 acaaaagaag aagattttat aaaacatctc cttctaacat caactactga tacacttttg
      481 tttttttctg ataaggggaa agtttactgg cttaaaggtt acttaattcc tgaagggagt
      541 agacaagcaa aagggaaacc aataataaat cttttaaaaa tagaacccgg agaaaaaatt
      601 tctgcagtaa tccctgtaag agaattttca aaagacaaat ttttaattat gattacaaaa
      661 aatggacttg tgaaaaaaac atcccttgag aaatatagta aaccaagaaa aggtggaata
      721 ataggtattg atttaagaga aggagataaa cttatagatg ttaaacttac agatggaaat
      781 agtcatataa ttttaagtac taaaaatggt ttaagcatca gatttccaga aaaagaagta
      841 aaaccggttg gaagaaatgc tattggcgta ataggaataa gatttaagaa aaaagatgat
      901 gaagttgttg gttgcgaaat tataaatcca gaagaagaga atttttcttt gcttactgta
      961 tgtaaaaaag ggtttggaaa gagaacatta attaaagaat ataaagttca acatagagga
     1021 ggtaaaggag ttattgatat aaaagttggt aacaaaaatg gtgaggttgt tggttctaaa
     1081 cttgtttctg gagaagatga aataataata actaccaaaa agggtattgt tataagaatt
     1141 aaagtttctg acataagaat tgttggaaga aatactattg gtgtgaaact gataaatctt
     1201 ccttctgatg atgaaataac agatataacc aaagtggaaa aagaaaataa tgaataaaaa
     1261 tattgaaatt ataaaccata ccgccgatac aggaataatt gtcaaaggaa aaacaataga
     1321 agaaattttt attaattctt tgaaaggact ttatgaaata atgggagtga aatcaaacgg
     1381 gaaagaaagt attatggaaa taaacttgaa atcagaggaa cttgaaaatt tacttgttaa
     1441 atttttgaat gaaatgatat attatatgga aacaaaaaaa atgtcaggtg aaatcaaaga
     1501 aattaaaata gattttaaaa ataatgaata taatctcttt gcaaaactta aaatgcaaaa
     1561 agtagaaaaa atagaaaaag aagttaaagc aacaacatat cacaatttga agataaaaaa
     1621 attagataac cagtatacaa caacaataat ttttgattta taaatgagag ggaagccatg
     1681 aaaaaagaag aaaaaaatta ttttcttaaa ttacttgaaa gagaaaaaga aaaaattttt
     1741 aaaaatttat cttacgcaag agaagtaatt gaaaaaggag aaaaaggggt tccaactcat
     1801 attgcagatt atggaactga tgaatttgaa aaagaccttg aagttgggct ttctaattct
     1861 gagagtaaat tacttgaaga aatcaatata gcaataaaaa aaattaaaga tggaacttat
     1921 ggaaaatgtg acaactgtgg acaactaata tcaaaagaaa gattaaaggc aattccatat
     1981 gcaagatact gcataaagtg tcaaacggaa agagaaaaaa agtgaaattt agattttttt
     2041 taaaaatttt tttaacaact tcatttttag cacttttttt tgatcagata acaaaatctt
     2101 ttatagttag attactgaaa aaaactgatg gaaatataaa tattacctct ttcctttctt
     2161 ttacacttat aaaaaatcaa ggaattgttt ttggactatt caataatgaa aaaacgagaa
     2221 tctttataat aattttttct tttattgcaa ttttttttat tatttttgcc attttaaaat
     2281 taaaaaaaac agaaaaatat catcaatttt ctctcggttt gattgttgga gggacaatag
     2341 gaaatctact tgatagaata agatttggat atgttattga ttttataaat ttccacttct
     2401 ggccagtttt taattttgca gatgctttca tatcaatagg tgttatttta ttctttataa
     2461 aatatttaaa ggaataaaaa tgcatccagt atttataaaa attgggaatt ttgttatata
     2521 ctggtatgga gtttttgttg ctgttggagt ttttatttca atagcattat ttgaaaaaga
     2581 atatcataag gaaggttacg atgaaaagtt tatctctcaa ttagttttct tgacaatttt
     2641 tactggaatt attggcgcaa gatttttaca tattctggta aatatatatt attacattct
     2701 tcatccactg gaaattatta aaataaggaa tggagggctt gctgttgaag gagcaattct
     2761 atcttcttta atttttatta ttttattttc taaaataaaa aatttttcac cagttaaact
     2821 tcttgataca attgcaattt atgttcctat tggacaggca attggtagat taggatgttt
     2881 tttaaacggt tgctgttatg ggaaagaaac agatttcttt atcggggtaa aatttccttt
     2941 tatggataaa aaagtacatc ctacgcaact ttattattca tttttgtaca tagtactttt
     3001 tttcttttta aagaaattat caaaaaaaac aaaaaaagga gaaggattta tattttcttc
     3061 ttatttaata ggtttttctt taataaggta ttttgttgac tttttaagag gggatttaaa
     3121 aagaacatca cttggacttt attcaactca attcattgca attttaatat ttatgatagg
     3181 tggaatttat attattgtaa aattaataaa agggaggaaa taaatggcta aacttgtttt
     3241 tgattatccc tttatgaata gagaatatga gttggttaaa gaaaaaattt atgttggaag
     3301 gcagaataca aatgatataa ccataccgga ttatcaaatt tttcaatctt taccagaatc
     3361 cacaaaaaga ttgtatcttg acagtttagt taaagtatca agaatccatg caagaataat
     3421 caaaaaagat gatggttggt atattgaaga tattggaaca catggacttg gaagtcaata
     3481 tggtacatat gtcaacatgg ctcgtattga agttaaaaaa ccttataaac ttcaggaaaa
     3541 cgacgaaata cagtttggcc ccattaagtg taaatttttt gaaaaataat tctgaaactt
     3601 ctacaaaaaa ataccatcta aataaaaaaa ataaaaggag agtaaaaaat gaatcttgaa
     3661 aaatttacat tgaaagctca agaagcatta atgtcttcac aggctttaac tcaagaaaaa
     3721 aataataatg aagtagatgt attacatctt gcatatgtat tggtagatag cgatgaaacg
     3781 attattgaca ttttaaaaga aatgaatgta gatgtggaag gaataaaaaa agatattgaa
     3841 aaagaaatag ataaacttcc aaaagtttat aattcaaaaa ctgctttata tataagtcct
     3901 ttactaaatc aaactattta taaagcacaa aattatgcca aaatttttga agatgaatat
     3961 attggagtgg aacatctttt tatgctataa gtgaagttga ttctcctgtg aaagaaattt
     4021 ttgcaaaata taatattttt aaggatagaa taataaaaat tatacaaaat ttaagaaatg
     4081 gagaaaagat tactgataga gatgctgaaa ctaaatataa agtccttgat aaatttacaa
     4141 gagatttgac agatttagca agaaaaggga aacttgaccc tgttattgga agagatgagg
     4201 aaattagaag agttatacaa gttttatcaa gaagaactaa aaataatcct gttttgattg
     4261 gagaagcagg tgttggtaaa acagcaatag ttgaaggtct ggcaaggaga attatttcag
     4321 gagatgtacc tgaaaattta aaaaataaaa aaatacttgc tcttgatctt ggtgctttaa
     4381 ttgctgggac gaaatttaga ggagaatttg aggatagatt aaaagcactt ttaaaagaaa
     4441 tagaaaaaaa agaaggtgag ataatacttt ttattgatga attacatact cttgttggag
     4501 caggtgcagc agaaggagca attgatgctt ctaacatgct aaaacctgca cttgcgaggg
     4561 gaactttaag atgtattgga gccacaactc ttgatgaata caggaaatat atagaaaaag
     4621 atagagccct tgaaagaaga tttcaacctg tttatgtaaa agaaccaact gttgaagaaa
     4681 caatatcaat tttaagggga ttgaaagaaa aatatgaagt tcatcatgga gttaaaataa
     4741 ctgatagtgc aattgttgca gcggcggttt tatcacatag atatataagt ggaagatttt
     4801 taccagataa agcaatagat
//
LOCUS       DAOSAT010000112         4721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_11423_length_4721_cov_5.442135, whole genome shotgun sequence.
ACCESSION   DAOSAT010000112 DAOSAT010000000
VERSION     DAOSAT010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11423_length_4721_cov_5.442135"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLF90_08400"
     CDS             <1..302
                     /locus_tag="PLF90_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK57330.1"
                     /translation="EAGVDILNPVQTSAANMEPKRLKEKFGNKIVFWGGGCDTQHVLG
                     RATPEEIQQHVKERLDIFKKGSGYVFNQVHNIQANVPPENIVAMLESAYKYGKNK"
     gene            complement(405..476)
                     /locus_tag="PLF90_08405"
     tRNA            complement(405..476)
                     /locus_tag="PLF90_08405"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(442..444),aa:Ile,seq:cat)
     gene            complement(515..1918)
                     /locus_tag="PLF90_08410"
     CDS             complement(515..1918)
                     /locus_tag="PLF90_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HOK57331.1"
                     /translation="MEKIGLGLIIGNRGFFPDHLCKEGREKILNFLSKRFEVITLTEK
                     DTKFGSIETYQDAKKCADLFIKNRDKIDGIIVSLPNFGDERAVADTIKLSGLSVPVLI
                     HAFPDDIDKMDVKNRRDSFCGKISVCNVLYQYGIKFTLTENHTEGIESDEFKRDIDKF
                     ERICRIVKGLKNLKLGAIGTRPDAFKTVRYSEKILEKNGISVDVLDLSEIFARISRIK
                     DDDKRVKNEIEKFRNYMESKDVPLQSLIKFSKLKIVIKDWISQNDIKGIGFQCWTSIE
                     ENFGIVPCAVLSYLSDTLIPSACEVDITGTLSMYILQIASGLPSAIVDLNNNYKEEKD
                     KVVLFHCSNFPVSVLKKSKISYQEIISESVGKENAYGAVIGNMKKSPFTFLRLSTDDN
                     FGIIKGYLGEGEITDDKIETFGGIGVAKINNLQKLLWFICENGFEHHTIINLSEISDA
                     VEEALVKYKNWSIYHHY"
     gene            complement(1932..3038)
                     /locus_tag="PLF90_08415"
     CDS             complement(1932..3038)
                     /locus_tag="PLF90_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK57332.1"
                     /translation="MNKRERVERTLNLKEVDRVPVYDLVRNDDVFEYFSGEKLPPEEM
                     WEKEEEKLKIIAGKVVNKLCDMTRSFGFGPLKETDYTDEFGFVWHSSPYEKTSWIVKR
                     PFYDEKDGIEFIKKLIIKYSENIKEIKSNQKAYREKYHNDFLKTQSYIGDTINLLAEQ
                     GTGLDLLRHYLGFEFFSYIYAENPDIISEFFEVYTDLQVLICHTIADRNLSPCVLTYG
                     DIACKNMLLHSPQFLRKEFFPRLKKIQDAWHQYNIKCLFHSDGNIMEVLDDLIETGID
                     GLNPIEIVAGMDIKKIKEKYGNKIFLTGGIDMSQLLSLKTTEEVKEKCIETIKICYPG
                     YFIGSTTEIDNSVKLENIIVMYEIANQPVEKILK"
     gene            3130..3960
                     /locus_tag="PLF90_08420"
     CDS             3130..3960
                     /locus_tag="PLF90_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK57333.1"
                     /translation="MKFTGISDEAGQSIEVQIKAHKELGWEYMELRNIDKENLTMMSD
                     EKFEIVYKKVNEAGMKVSCFASCIANWATRISGDFKKDYDELKRAIPRMKRFNTKYIR
                     VMSWPNDTENPWDEERWAKEVIRRMKELVKMAEDNGIIIVHENCSGWGGESAENMVRL
                     VEEMNSQNFKLMFDTGNVVAHNKNVDPWDFYMKVKPFIEYVHIKDYNKEGRATFPGEG
                     EAKVKEILNDLKKSGYDGFVSIEPHLASVVHEGKVGDPEITYKTYITYGRKLMALINP
                     "
     gene            complement(3967..4515)
                     /locus_tag="PLF90_08425"
     CDS             complement(3967..4515)
                     /locus_tag="PLF90_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57334.1"
                     /translation="MKKRFLIYTIILISGILSIKFYESRIINVLWLKADIAFHFGNYD
                     LAEKCLWRIIKQKPEEVDAYVLKAWLEWSQALSKNDNKKLNSAIKTLKLGQWYNPFDI
                     RLYMEEGIMWNAFGNDEAALNAFKKIYVIGTIPYVRMYPHKLRQMGKIYEAYKVMKKI
                     YEKYKDDVTFQCLERLKKELSA"
     gene            complement(4526..>4721)
                     /locus_tag="PLF90_08430"
     CDS             complement(4526..>4721)
                     /locus_tag="PLF90_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOK57335.1"
                     /translation="YQDVELSHEAAIGKIKKEEIEYLASRGIPEEEAQAMIIRGFMNV
                     EILGLPATLQKEIKKVTEKV"
BASE COUNT         1553 a          804 c          634 g         1730 t
ORIGIN      
        1 ttgaagcagg ggttgatatt ctaaatccag ttcaaacatc agcggcaaat atggagccga
       61 aaaggttgaa agaaaaattt ggtaataaaa ttgttttctg gggtggggga tgtgatacac
      121 agcatgtttt aggcagggca acaccagaag aaatccaaca acatgtaaaa gaacggctgg
      181 atattttcaa aaaaggcagt ggttatgttt tcaatcaggt tcataacatt caagcaaatg
      241 ttccaccaga aaatattgtt gctatgcttg aatctgccta taaatatgga aaaaacaaat
      301 agtcaataat gtctgttgta atctttgtct ctcaatcatc tccactgccc accttaaagg
      361 tgtcctctga agatgaattt ggtttttagt tctcacaaaa gaagtggacc cgggcggatt
      421 cgaaccacca accaacggat tatgagtccg ctgctctgcc gttgagctac gggtcctctt
      481 tttattacat attatactac tatttttaat tttttcaata gtggtggtag attgaccaat
      541 ttttatattt cacaagtgct tcctcaactg catctgaaat ttctgataaa tttatgatag
      601 tatgatgctc aaaaccattt tcacatataa accataaaag tttctgtaaa ttatttattt
      661 ttgctactcc aatccctccg aatgtctcta ttttatcatc tgttatctct ccttctccga
      721 gatatccttt tataattcca aaattatcat ctgttgaaag ccgtaaaaaa gtgaaaggtg
      781 attttttcat atttcctata acagcaccat aagcattttc ctttcccaca ctttctgata
      841 ttatttcctg ataacttatc tttgatttct ttaaaacaga aacaggaaaa ttactgcagt
      901 ggaataaaac aactttgtct ttctcttctt tataattatt atttaaatca actatagcac
      961 ttggaagacc tgaagcaatc tgaagaatat acatactcaa tgtacccgtt atatcaactt
     1021 cacaggcaga aggaattaaa gtgtctgaca gataacttaa tacagcacac ggaactatac
     1081 caaaattttc ttctattgaa gtccagcact gaaatccaat accttttata tcattctggg
     1141 aaatccagtc ctttattaca attttaagtt ttgaaaattt tattaatgat tgaagcggaa
     1201 catctttact ctccatataa tttctgaatt tttctatttc attttttact cttttatcat
     1261 catcctttat tcttgaaatt cttgcaaaaa tctctgataa atcaagaaca tcaacactta
     1321 ttccattttt ctcaagtatt ttctcactgt atctgactgt tttaaatgca tcaggtcttg
     1381 tccctattgc tccaagtttt aagtttttaa gtccttttac aattctgcat attctttcaa
     1441 atttatctat atccctttta aactcatcac tttcaattcc ttcagtatgg ttttctgtta
     1501 atgtaaattt aattccatac tgatataaaa cattacagac agaaatttta ccgcagaaac
     1561 tatctctcct gttttttaca tccattttat ctatatcatc aggaaaagca tgtattaaaa
     1621 caggaactga aagacctgaa agttttattg tatctgctac tgctctttca tctccaaaat
     1681 ttggtaaact tacaattata ccatctattt tatctctgtt ttttataaat aaatccgcac
     1741 acttttttgc atcctgatat gtttcaatac tcccgaattt tgtatctttt tctgtcaatg
     1801 ttatcacttc aaatctttta ctcaaaaaat tcagaatttt ttctctccct tctttacata
     1861 aatggtcagg gaaaaaaccc ctatttccta ttatcaaacc taatccaatt ttttccattt
     1921 tctccccctt tttattttaa tattttttca acaggttgat ttgctatttc atacattact
     1981 attatatttt caagttttac agagttgtca atttctgttg ttgaaccaat aaaatatcca
     2041 ggataacaaa tttttattgt ttctatacat ttttccttca cttcttcagt tgtttttaaa
     2101 gacagtaact gactcatatc tattccacct gtcagaaaaa ttttatttcc atatttttct
     2161 tttatttttt ttatatccat tcctgcgact atttcaatcg ggtttaaacc atctatacct
     2221 gtttctatta aatcgtccag aacttccatt atatttccat ctgaatggaa taaacatttt
     2281 atattatact ggtgccatgc atcctgaatt tttttcaatc ttgggaaaaa ctcttttctt
     2341 aaaaattgag gagaatgcaa taacatgttt ttacaggcaa tatctccata agttaaaaca
     2401 catggcgaaa gatttctatc tgcaattgta tgacatatta aaacctgtaa atctgtatat
     2461 acttcaaaaa attcactgat aatatcagga ttttcagcat agatatagga aaaaaattca
     2521 aatccaagat aatgtcttaa taaatcaagt cctgttccct gttcagcaag aaggttaatt
     2581 gtatctccta tatatgattg ggtttttaaa aaatcgttat gatatttttc tctgtatgct
     2641 ttctgatttg attttatttc tttaatattt tcagaatatt taattatcag tttttttata
     2701 aattcaattc catctttttc atcatagaaa ggtcttttaa caatccatga agttttctca
     2761 taaggacttg aatgccatac aaaaccaaat tcatctgtat aatcagtttc tttcaaagga
     2821 ccaaaaccaa aacttcttgt catatcgcat aatttattta ctacttttcc tgctataatt
     2881 ttcaattttt cttcttcttt ttcccacatt tcttctggag gcaatttttc accactaaaa
     2941 tattcaaaaa catcatcatt tctgacaagg tcataaacag ggactctatc aacttctttt
     3001 aaatttaatg tcctttcaac tctttctctt ttattcataa gaaaatattt tacaatttta
     3061 aaatttaaag agcaaaaaat tgacattgca taaataatga ttaaaattat taaaaacaag
     3121 gaggacaaaa tgaaatttac gggaatcagt gatgaagcag gacaatcaat tgaagttcag
     3181 attaaagcac ataaagaact tggatgggaa tatatggaat taagaaatat tgataaagaa
     3241 aatttaacta tgatgagtga tgaaaaattt gaaattgttt ataaaaaagt aaatgaagcg
     3301 ggaatgaagg tttcctgttt tgcaagttgt atagcaaact gggcaactag aatttctgga
     3361 gatttcaaaa aggactatga tgaacttaaa agagcaatac ccagaatgaa aagatttaac
     3421 acaaaatata taagagttat gtcatggcca aatgatactg aaaacccatg ggatgaagaa
     3481 agatgggcaa aagaggttat aagaagaatg aaagaattgg taaaaatggc tgaagataat
     3541 ggaataatta ttgttcatga aaactgttcc ggatggggag gagaaagtgc tgaaaatatg
     3601 gtaagattgg ttgaagaaat gaactctcaa aattttaaat tgatgtttga tacaggaaat
     3661 gttgttgccc ataataaaaa tgttgacccc tgggattttt atatgaaggt taaaccattt
     3721 attgaatatg ttcatattaa agattacaat aaagaaggaa gggctacttt cccgggagaa
     3781 ggagaagcta aagttaaaga gattttaaat gacttgaaga aaagtggata tgatggtttt
     3841 gtttcaatag aaccgcatct ggcaagtgtt gttcatgaag gaaaagttgg agaccctgaa
     3901 attacatata aaacatatat tacttatgga aggaaattaa tggctctgat aaatccataa
     3961 aaaagtttat gctgatagtt cttttttcaa tctttcaaga cactggaaag taacatcatc
     4021 tttatatttt tcatatattt ttttcattac cttatatgct tcatagattt tacccatctg
     4081 cctcaattta tgcggataca ttcttacata cgggatagta ccaattacat aaatcttttt
     4141 aaatgcgttc agtgctgctt cgtcattccc aaaagcattc cacattattc cttcttccat
     4201 ataaagacgg atatcaaatg gattatacca ctgtccaagt tttaatgttt ttattgctga
     4261 attcaatttt ttattatcat tttttgataa tgcctgactc cattcaagcc atgcttttaa
     4321 aacatatgca tctacttctt ccggtttctg tttaattatt ctccacagac atttttcagc
     4381 aaggtcataa ttaccaaaat gaaaagctat atctgccttc aaccacagta catttattat
     4441 ccttgattca taaaatttaa tactcaaaat ccctgaaatt aatataatgg tgtaaattaa
     4501 aaatcttttt ttcatttctt aactttcaaa ctttttctgt aactttcttt atttcttttt
     4561 gaagagttgc aggtagtcca agtatctcaa cattcataaa acctcttatt atcattgcct
     4621 gtgcttcttc ctcaggaatt cccctgcttg caagatattc aatctcttct ttttttattt
     4681 tacctattgc tgcttcatga gaaagttcaa catcctgata a
//
LOCUS       DAOSAT010000113         4425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_12430_length_4425_cov_6.405950, whole genome shotgun sequence.
ACCESSION   DAOSAT010000113 DAOSAT010000000
VERSION     DAOSAT010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12430_length_4425_cov_6.405950"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1725
                     /gene="rny"
                     /locus_tag="PLF90_08435"
     CDS             148..1725
                     /gene="rny"
                     /locus_tag="PLF90_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOK57336.1"
                     /translation="MPNSINTLKLVMPWFILWGAISILVGYLWRKYVGERTIKSAEKK
                     AKEIIQEAEDIALKKKREIDIEAKELFYRLRSDFEKETKNKRKELQFQEKRLQQKELA
                     IERKAEIIEIKEEELSKREKEIQRREEEIKNKEMEYLQLIEEEKKKLEAISGISREEA
                     KNQLLKIMEEEAKKEAIKLQQKIENEVRENAEKKAREILLTTMQRLAPEEATESVVSV
                     VSLPSDEIKGRIIGREGRNIKVFEALTGVDLIVDDTPEAVTISCFNLYRRELARQALE
                     KLIADGRIHPARIEEIVEKTKKELEEQLVEDGNRTLLEIGIENVHPELVKLLGKLKYR
                     TSYGQNLLIHSKECAFMAGIIASELGYDPKIAKRAALFHDIGKAVDQEVEGAHPEIGG
                     EILKKYGESEEIINAALNHHNDMPINEPYTVISQIVDALSATRTGARRDTLEKYLRRI
                     EELEKIASSFKGVDSAYAISAGREVRVIVKPEEITDDEARTLAKEIAKKIEENMEYIG
                     QVKVTVIRERREVEFAK"
     gene            1725..2387
                     /locus_tag="PLF90_08440"
     CDS             1725..2387
                     /locus_tag="PLF90_08440"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HOK57337.1"
                     /translation="MEVKKLHRWDIDKEEAEKIQERLKDKVVIKPLSKKINIICGIDS
                     SYNEETIQTCGVLCKFPELEIIEIVKIKGKVKFPYIPGLLAFREGENTVNLIEKIEKE
                     VDCFIFDGHGIAHPKKFGFASHIGVLIEKPTIGCAKSLLYGNYFEPPSFPLSSTFIKD
                     TEGEIIGHALRNYNDGIIFISPGHLIDFKDSLSTVIKCMKEKYSIPYPLYLAHENSKL
                     TD"
     gene            complement(2342..3898)
                     /locus_tag="PLF90_08445"
     CDS             complement(2342..3898)
                     /locus_tag="PLF90_08445"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK57338.1"
                     /translation="MGLWNLNRFPFNPETLSDGLKKGTLYKILNSYMTKRVLLKYLKE
                     DENGKTKLEEFIEKFSIDTRGILERIFKEKETIVPYMIFDHICKIFSIERKELKEAMK
                     NPVIKRSVFNIAETVIKYGVREPLIFAEPLMVVWNITGRCNLRCKHCYEDAGVLSKGL
                     PDELTNEEKIKVMEEIVKTNIPTLAFAGGEPLMDPVFWELAKIGKEGGLYMSINTNGT
                     MITKKVAERLKEIGFAYYGVSLDGASPEIHDSFRGVNGSFEKTLNGIKNLVEVGEADK
                     VCISFTVARENAIVKNQVPEMIKLRDQLGIRKVVLYNYIPCGRADFGNDLTCEEREKL
                     FELFYEDLKTGKETLLSTAPQFGRYCKQMYELGEGDFTIIGHFTSGNVKKLKNIVELI
                     GGCGAGRAYIAIQPDGSITPCVFMPDVYIGSIKENGEIKKENLLNVWDNSEVLNIIRD
                     RRNHPERYGCKGKYFSVCGGCVARSYAYFGDFVSPDPGCILNKEIIESRKNKKYQSVN
                     FEFSCARYKG"
     gene            complement(3911..4375)
                     /locus_tag="PLF90_08450"
     CDS             complement(3911..4375)
                     /locus_tag="PLF90_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK57339.1"
                     /translation="MAKHLFAEIKKEDVKKLFEFLKDEKEYIKKRAHLIILSGIHRYK
                     VSEITKIIKIHPNHLRKWIHRYNKHGLESIVESPETGKKKKFKENIKNRIIEIVKTPP
                     RKLGLLFSMWTLYKLKNYLEDKGIVEGISHETIRRILKETNIDLKKMKYEEN"
BASE COUNT         1627 a          705 c          665 g         1428 t
ORIGIN      
        1 gtaaggatat aaaaaaaatg gaactaaaaa ttaaaataga taaaaaacat agtaacaaat
       61 ttttaaagat tatttttcta gagttccttc ctgtctgtcg tgctggataa ctcctttact
      121 taatttttaa aagtaaagga gggcaaaatg ccaaattcaa taaatactct caaacttgtt
      181 atgccctggt ttattctctg gggtgcaata tcaatacttg tgggatattt atggagaaaa
      241 tatgttggag aaaggacaat taaatcagca gaaaagaagg caaaagaaat catacaagaa
      301 gcagaagata tagcattaaa gaaaaagagg gaaatagata tagaagcaaa agaattgttt
      361 tacagattaa gaagcgattt tgagaaagaa acaaaaaaca aaagaaaaga attacagttt
      421 caggaaaaaa gattacagca aaaagaactt gcaattgaaa gaaaagcaga aattattgag
      481 attaaagaag aggaattatc aaaaagggaa aaagaaatac agagaagaga agaagagatt
      541 aaaaataagg agatggaata tttacaactc atagaagaag aaaagaaaaa acttgaagca
      601 atttccggta tttcaagaga ggaagcaaaa aatcaacttt tgaaaataat ggaagaggaa
      661 gcaaaaaaag aagcgataaa attacagcaa aaaattgaaa atgaagtaag agagaatgct
      721 gaaaaaaaag caagagaaat tttattgacc acaatgcaac gattagcacc tgaagaagcg
      781 actgaaagtg ttgtttctgt tgtcagttta ccatccgatg aaattaaagg aaggattata
      841 ggaagagaag gaagaaacat taaagttttt gaagcactta caggagtaga cctgatagtt
      901 gacgatactc ctgaagcggt tacaatttcc tgctttaatc tttatagaag agaacttgca
      961 agacaggcac ttgaaaaact tattgcagat ggaagaatac atccagcaag aattgaagag
     1021 attgttgaaa agacaaaaaa agaacttgaa gaacaacttg tagaagatgg aaacaggaca
     1081 ttacttgaaa taggaataga aaatgtacat ccagaacttg taaaattact tggtaaatta
     1141 aaatacagaa caagttatgg acaaaatctt cttattcaca gtaaagaatg tgcatttatg
     1201 gcaggaatta ttgcttctga actcggatat gacccaaaaa ttgcaaaaag agcagcactt
     1261 tttcatgata taggaaaagc agttgaccag gaagttgaag gtgcacatcc tgaaattggt
     1321 ggagaaattt tgaaaaaata tggagaatct gaagaaatta taaatgctgc tcttaatcat
     1381 cacaatgaca tgccaattaa tgaaccatat acagttatat ctcagattgt agatgcactt
     1441 tctgctacaa gaacaggagc aagaagagat actcttgaaa aatatttaag aaggatagaa
     1501 gaacttgaaa aaattgcttc ttcttttaaa ggggtagatt ctgcttatgc tatatctgct
     1561 ggaagggaag tcagggtaat agttaaacct gaagaaatca cagatgatga agcaagaacc
     1621 ctcgcaaaag aaattgcaaa aaagattgaa gaaaacatgg aatacatagg acaggtgaaa
     1681 gtaactgtca taagagaaag aagagaagtg gaatttgcta aataatggaa gttaaaaagt
     1741 tacacaggtg ggatatagat aaagaagaag cagagaaaat acaggaaagg ttaaaagata
     1801 aagttgtgat aaaaccctta tcaaaaaaaa taaatattat atgcgggatt gatagtagtt
     1861 ataatgaaga aacaatacaa acatgcggtg ttttatgtaa attccctgaa cttgaaataa
     1921 tagaaattgt aaaaattaag ggaaaggtga aattccccta tattccggga cttcttgcat
     1981 tcagggaagg tgaaaataca gtcaatctta tagaaaaaat agaaaaggaa gtggattgtt
     2041 ttatttttga tggacacgga attgctcatc ctaaaaaatt tggttttgct tctcacatag
     2101 gagtactgat tgaaaaacca actattggat gtgcaaagtc cttactttat ggtaattatt
     2161 ttgaaccacc ctctttcccg ctttcatcaa catttataaa agacactgaa ggagaaataa
     2221 ttggccatgc tttaagaaat tacaatgatg gaataatttt tatttctccc ggacatttaa
     2281 ttgattttaa ggattcttta agtactgtaa tcaaatgtat gaaagagaaa tactctatcc
     2341 cttatccttt atatcttgca catgaaaact caaagttaac tgactgatac tttttgtttt
     2401 ttctactttc aattatctct ttattcagaa tacatccagg gtcaggagaa acaaaatcac
     2461 caaaataagc ataacttctt gcaacacatc ctccacaaac agaaaaatat ttacccttac
     2521 aaccatatct ttcaggatga tttctcctat cccttattat attcaaaact tctgaattat
     2581 cccacacatt caaaagattt tcctttttaa tctctccatt ttcttttata cttcctatat
     2641 aaacatcagg cataaaaaca catggagtta tacttccatc aggttgaatt gctatatatg
     2701 ctcttcctgc tccacagcca ccaataagtt caacaatgtt ttttagtttt tttacattac
     2761 cagatgtaaa atggccgatg atagtaaaat ccccttctcc aagttcatac atctgtttac
     2821 agtatctacc aaattgaggc gcagttgaaa gtaaagtttc ttttccagtt ttcaaatctt
     2881 cataaaacaa ttcaaacaat ttttctcttt cctcacacgt taaatcattt ccaaaatcag
     2941 cccttccaca tggtatataa ttatataaaa caacttttct tattccaagt tggtctctca
     3001 atttaatcat ttcaggtact tgatttttaa caattgcatt ttctcttgca acagtaaagg
     3061 agatacaaac cttgtctgcc tccccaactt ctactaaatt tttaattcca ttcaacgttt
     3121 tctcaaatga accattaaca cctctaaaac tatcatgtat ttcaggactg gcaccatcta
     3181 aacttactcc ataatatgca aaaccgatct cctttaatct ttctgccact ttttttgtta
     3241 tcatggttcc atttgtattt attgacatat ataaaccgcc ctctttacca atctttgcaa
     3301 gttcccagaa aacaggatcc atcaatggtt ctcctcctgc gaatgcaaga gttggaatgt
     3361 ttgtttttac tatttcctcc ataactttta ttttttcctc attagtcagt tcatcaggaa
     3421 gtccttttga taaaacacct gcatcttcat aacagtgttt acatctcaaa ttacatctac
     3481 ctgtaatatt ccatacaacc attagtggtt cagcaaagat aagtggttct cttactccat
     3541 attttataac tgtctcagca atattaaaaa cagacctttt tataaccgga tttttcattg
     3601 cttctttcaa ttcttttctc tcaatagaaa aaattttaca tatatggtca aaaatcatat
     3661 aaggaacaat ggtttccttc tccttaaaaa ttctttctaa aatcccccgt gtatctatac
     3721 tgaacttttc aataaattct tcaagtttag tttttccatt ttcatcttct ttaaggtatt
     3781 ttaacagaac cctttttgtc atataactat taagaatttt ataaagtgtc ccttttttta
     3841 aaccatcaga aagggtttca ggattaaaag gaaatctatt taaattccag agtcccattt
     3901 tccacctcct ttaattttct tcatatttca tttttttcaa atctatattt gtttctttaa
     3961 ggattctcct tattgtttca tgacttattc cttcaacaat ccctttatct tcaagataat
     4021 ttttcaactt ataaagagtc cacattgaaa ataaaagtcc gagttttctc ggaggtgttt
     4081 taacaatctc tattattctg ttttttatat tttctttaaa tttcttcttt ttacctgttt
     4141 caggagattc aacaattgat tccagaccat gtttattata tctgtgaatc cattttctta
     4201 aatgatttgg atgaattttt ataatttttg ttatttcaga aactttatat ctatgaatac
     4261 ccgaaagaat tataagatgc gctctttttt taatatattc tttttcatct tttaaaaatt
     4321 caaataattt tttaacatct tcctttttaa tttcagcaaa taaatgtttt gccatttaac
     4381 cccctttttt gaaattatta taccctgttt tttgaaattg tcaag
//
LOCUS       DAOSAT010000114         4333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_12763_length_4333_cov_6.070126, whole genome shotgun sequence.
ACCESSION   DAOSAT010000114 DAOSAT010000000
VERSION     DAOSAT010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12763_length_4333_cov_6.070126"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PLF90_08455"
     CDS             <1..591
                     /locus_tag="PLF90_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57340.1"
                     /translation="NWPAIEYHNGKIKENWSRPVSPEKPMPDIFIHFRIAAYLAGLGE
                     IGYSKILLTPQFGPRQRFACILTDAPLEPDPIYDGPPICDRCMLCVKECSAGAISRTE
                     TVKLKLPGIGGKEIEWGKLDIEKCTNGFQGKEPYKKYNPFIGEPCPVIIYSRALEGAR
                     GCIRACMIHLEKEGKIKNKFKEPFRKRKAWYIEWEK"
     gene            579..1604
                     /locus_tag="PLF90_08460"
     CDS             579..1604
                     /locus_tag="PLF90_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOK57341.1"
                     /translation="MGKIKYSVGYQLREEENFSDIVEKFKDRIEEVYFPWLNLPSGRG
                     PIADKSGYINWEAQKQLEYEIKKIKKNGIKLNLLLNASCWGGSSLSISHVNTVISIVD
                     YLCENIGIDSVTCFSPVVAYIIKKDFPEIEIRASVNMRIGTKRGMEQVKELFDSFVMQ
                     REYNRDFEKIEEMKEWCDKNGKKLSILVNSGCLNFCAVQLFHDNVISHEAEVFTKANI
                     TDEIPGNCWSFYRNTENWKYLITSSWIRPEDIHYYEKYFDVVKLATRINPYPEKVIEA
                     YYNEKYDGNLIDLFEPSHSKILYPYIIENSKFPENWFKKILSCDKNCEKCFYCSDIFK
                     KVLVKKL"
     gene            1620..2588
                     /locus_tag="PLF90_08465"
     CDS             1620..2588
                     /locus_tag="PLF90_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK57342.1"
                     /translation="MKLTAEMVKEKAKSLGADVVGIGNIERWEGTPIQMDPKQIMPDA
                     RSVIAMGFRIMRGSLRGIEEGTFFSNYSSMGYGGLTYLYIPIVVINLSKFIEDFGYEA
                     IPYGHQSDWRAIDNEGNLKPNYSKPVTSGKANPDVMVHLRIAAYLCGLGEIGYSKLLL
                     TPEFGPRQRIGIVITEAPLEPDPLIEPGTLCDRCMACVKECPGNAISRNETVKVVLAE
                     KKVEWGKLDCKACDIAFRGGKPIKPEEDDGSLPDYMDNLLYGYKIKPNYITPFYRKPH
                     NLYNTGQAICGARCMISCMIHLEKKGILKNKFEKPFRRKEPWKVNW"
     gene            2602..3459
                     /locus_tag="PLF90_08470"
     CDS             2602..3459
                     /locus_tag="PLF90_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57343.1"
                     /translation="MDKNKLKEYAKEVKADLVGIAGIDRFKGIDKQHHPESIFPEVKS
                     VIVIGKRVTRGALRGIEEGTQFGLYNLYGYHWLNNRILALTTFKICEFLEDNGYEAVP
                     LPDIPSEIPPMGIPIRKGKPAPNVLIDIEDASVRAGLGEIGYCGIFLTPEFGPRQRFQ
                     IILTDAEIEPDPVKKENVCDLCKECIKICPLEAIKEKEKEIKICGKKMLISEIDYEKC
                     KKCKNGVFPNMFYENAKPDRIAALCSRTCLSHLEEKGKIKNLFNEKFRKRSVWKIDKS
                     GEIIEGEEK"
     gene            3456..>4333
                     /locus_tag="PLF90_08475"
     CDS             3456..>4333
                     /locus_tag="PLF90_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57344.1"
                     /translation="MTKLTKEMIRQAGYEFGLDLVGFANIDRFKDAPIHMHPKSIFPE
                     CKTVIVVGKRIVRGGWRGIEEGTNWISYTYFDYHGLLNTFFIPEPLYQLACFIEDFGY
                     EAVPYFPGVPEVQNPEVKPLRKGNVSPDVHLAIRIAAVCAGLGEIGWSKVFLSKKFGP
                     RQRLHAILTDLELEPDPLIKPGTICKKCMECVKDCPSKAIPHIKENKKIQIKIENYVY
                     EWADIHLGKCTLSYHGGDSRVSPFIHKDFPGWNIDARKQDFLEETAYKFCWTLSTGRW
                     RKTKEFPSGYIIEGHA"
BASE COUNT         1665 a          527 c          890 g         1251 t
ORIGIN      
        1 aactggccag caattgaata tcacaatgga aaaataaaag aaaactggtc ccgtcctgta
       61 tctccagaaa aaccgatgcc agatattttc attcatttca gaattgctgc atatcttgca
      121 ggtcttggag aaattggtta tagcaaaatt ttattaacac cccaatttgg gccaagacaa
      181 agatttgcct gtattttaac agatgcaccg cttgaaccag acccaatata cgatggacca
      241 ccaatctgtg atagatgtat gttatgtgta aaggaatgtt cagcaggagc aataagtcgg
      301 acagaaactg ttaaattaaa attaccggga atcggaggaa aagaaataga atggggaaaa
      361 cttgatatag aaaaatgtac aaatggtttt cagggaaaag aaccttataa aaaatataat
      421 ccatttattg gagaaccatg tcctgtcatc atatattcaa gggcacttga aggagcaaga
      481 ggttgtataa gggcatgcat gatacatctt gaaaaggaag gaaaaataaa aaataagttt
      541 aaagagccgt ttagaaaaag aaaggcatgg tatatagaat gggaaaaata aaatattcgg
      601 ttggatatca attgagagaa gaagaaaatt tttctgatat tgttgaaaaa ttcaaagata
      661 ggattgaaga ggtttacttt ccatggctta atttaccaag tggaagggga cctattgcag
      721 ataaaagcgg atatataaac tgggaagcac agaaacaact tgagtatgaa ataaaaaaaa
      781 taaaaaagaa tggaataaaa cttaatttac ttctaaatgc ttcctgctgg ggaggtagtt
      841 ctctttcaat cagtcatgta aatacagtta tttcaatagt tgattatctc tgtgaaaata
      901 taggaattga ttctgttaca tgcttttctc ctgttgttgc ttatataatt aagaaagatt
      961 ttccagaaat tgaaataagg gcaagtgtaa atatgaggat tgggactaaa agaggtatgg
     1021 aacaggttaa agaattattt gatagttttg ttatgcagag agaatataac agggattttg
     1081 agaaaattga agagatgaaa gaatggtgtg ataaaaatgg aaaaaaattg agtatacttg
     1141 taaacagtgg ttgtttaaat ttctgtgcgg ttcagttatt tcacgataat gtcatttctc
     1201 atgaagcaga agtattcaca aaggcaaata taacagacga aatacccggt aactgctgga
     1261 gtttttacag aaatacagaa aactggaaat atttaattac atcttcctgg ataagacctg
     1321 aggatataca ttactatgag aaatattttg atgtagttaa acttgcgaca agaataaatc
     1381 cttatccaga aaaagtaata gaagcatact ataatgaaaa atatgatgga aatttaattg
     1441 acctttttga accatctcac agcaaaattc tatacccata tattattgaa aactcaaaat
     1501 ttccggaaaa ctggtttaaa aaaattttat cctgtgataa gaattgtgaa aaatgttttt
     1561 attgttcgga tattttcaaa aaagtacttg ttaaaaaatt gtaataaagg agtaaaagta
     1621 tgaaactaac agcagaaatg gttaaagaaa aagcaaaaag tttaggagca gatgtggttg
     1681 gaattggaaa cattgaaaga tgggaaggaa caccaattca aatggaccca aaacagatta
     1741 tgcctgatgc aagaagtgta atagcgatgg gattcagaat aatgagagga agtttaaggg
     1801 gaattgagga aggaaccttt ttcagcaact attcttcaat gggttatgga ggacttactt
     1861 atctttatat tccaattgtt gttataaatt tgagtaaatt tattgaggac tttggatatg
     1921 aagcaatacc ttatggacat cagagtgact ggagggcaat agataatgaa ggaaatttaa
     1981 aaccaaatta cagtaaacct gttacttctg ggaaagccaa tcctgatgtt atggttcatt
     2041 taagaattgc tgcatatcta tgtgggcttg gagaaatagg atacagcaaa cttttattaa
     2101 cacctgagtt tggaccgaga cagagaatag gtatagttat aacagaggca cctttggaac
     2161 cagacccttt aattgaacca ggtactttat gtgatagatg tatggcatgt gttaaggaat
     2221 gtcctggaaa tgccataagt agaaatgaaa cagtaaaagt tgttcttgct gaaaagaaag
     2281 ttgaatgggg aaaacttgac tgtaaagcat gtgatattgc attcagggga ggaaaaccta
     2341 taaaaccaga agaagatgat ggttctctgc ctgactatat ggataatctt ctttatggtt
     2401 ataaaataaa accaaattat attactcctt tttatagaaa accccataat ctttataata
     2461 caggacaggc aatatgtgga gcaaggtgta tgataagttg tatgatacat cttgagaaaa
     2521 aaggaatttt aaaaaataaa tttgaaaaac catttagaag aaaagagcca tggaaagtaa
     2581 attggtaaaa aaggggttaa aatggataaa aataaattga aagagtatgc gaaagaagta
     2641 aaggcggatt tagttggaat tgctggtatt gatagattta agggtattga taaacagcat
     2701 catccagaaa gtatttttcc tgaagtaaaa agtgtaattg taattgggaa aagggtaaca
     2761 aggggtgcat tgagaggaat tgaagaagga acacagtttg ggctttataa tttatatgga
     2821 taccattggc taaataatag aatccttgct ttaactacat ttaaaatatg tgaatttctt
     2881 gaggataatg gttatgaagc agttccttta cctgatatac cttctgaaat tcctcctatg
     2941 ggaataccta taagaaaagg gaagccagca ccaaatgttt taattgatat tgaagatgca
     3001 tcagtaagag caggacttgg agagattgga tactgtggaa tatttttaac gcctgagttt
     3061 ggtccaagac agagatttca aattatactt acagatgcgg aaattgagcc agaccctgta
     3121 aagaaagaaa atgtatgtga tttatgtaaa gaatgcatta agatatgtcc tcttgaagca
     3181 ataaaagaaa aggaaaaaga aattaaaata tgtggaaaga aaatgttaat ttcagaaatt
     3241 gattatgaaa agtgtaaaaa atgtaaaaat ggtgttttcc cgaatatgtt ttatgaaaat
     3301 gcaaagccag atagaattgc tgctttatgc tcaaggacat gtctttctca tcttgaagaa
     3361 aaagggaaaa taaaaaacct ttttaatgaa aaattcagaa aaaggtctgt atggaaaata
     3421 gataaaagtg gagaaattat agaaggagag gaaaaatgac aaaattaaca aaggaaatga
     3481 taagacaagc gggttatgaa tttggtcttg atttagttgg ttttgcaaat attgatagat
     3541 ttaaagatgc acctattcat atgcatccaa aaagtatatt tcctgaatgc aaaacagtaa
     3601 tagttgttgg aaaaagaatt gtaaggggtg gatggcgtgg aatagaagag ggaacaaact
     3661 ggatttcata tacttacttt gattatcacg gacttttaaa cacttttttc attcctgaac
     3721 cactttatca acttgcctgc tttattgaag attttggtta tgaagcagtc ccctattttc
     3781 caggggttcc agaagtacaa aatccagaag taaaaccatt aagaaaagga aatgtgtctc
     3841 ctgatgttca tcttgctata aggattgctg cagtttgtgc aggacttgga gaaattggat
     3901 ggagtaaagt ttttttaagt aaaaaatttg gaccgagaca gagattacat gcaattttaa
     3961 cagaccttga acttgaacca gacccactta taaaacctgg aacaatatgc aaaaaatgta
     4021 tggaatgtgt taaagattgt ccttcaaaag caatacctca tataaaggaa aacaaaaaaa
     4081 tacagattaa aattgaaaat tatgtttatg aatgggcaga tatacatctt ggaaaatgta
     4141 cattgagtta tcatggaggt gattcaagag tttctccttt tatacataaa gattttcctg
     4201 gatggaatat agatgcaaga aaacaggact ttttagagga aactgcttat aaattctgct
     4261 ggactctgtc aactggaaga tggagaaaaa caaaagaatt tccatcaggt tatataattg
     4321 aaggacatgc aat
//
LOCUS       DAOSAT010000115         4040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_14045_length_4040_cov_5.757842, whole genome shotgun sequence.
ACCESSION   DAOSAT010000115 DAOSAT010000000
VERSION     DAOSAT010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14045_length_4040_cov_5.757842"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLF90_08480"
     CDS             complement(<1..387)
                     /locus_tag="PLF90_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK57345.1"
                     /translation="MEEKELVIIGGGPAGLSAAIEARKKGIDVLLIDENSKPGGQLFK
                     QIHRFFGSKEHMAGIRGFVIGERLLKEVKELGIDVYLNSVVYGIFKDKVIGIVRENKN
                     IILKAKYIIIATGAIENPLSFPGWTLP"
     gene            complement(366..701)
                     /locus_tag="PLF90_08485"
     CDS             complement(366..701)
                     /locus_tag="PLF90_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOK57346.1"
                     /translation="MKRKDFRIYKHPILGDLSERKKIYIYFNGKKLTAYEGETIASVL
                     YANGIRVLRKTEKFKQPRGIFCGIGRCTDCVMIVDGIPNVRTCITPVRDGMKVEFQKG
                     LGEWKKKNW"
     gene            complement(682..1740)
                     /locus_tag="PLF90_08490"
     CDS             complement(682..1740)
                     /locus_tag="PLF90_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK57347.1"
                     /translation="MEHIEIVKRAIEFKNPDYLPMELVDVPKIYNAYGTLNPDKVRFI
                     PGTEDFDSAWVTYHWTFIEEKNLGNGEILRKDEWNVWQKIPFDKNSTYIIIENPLADK
                     NNLSDYKFPSPEIADKFFERISKIIKENYSDRFICGYIDPGAFLIAYNIFGYQTFFIK
                     LVEDINFILDVISYIFEYHCSLIPKWKKAGAHMVNIIDEIAGNKGLFFNPQIWQKYFR
                     PFYEKLFRKIHQENMYTGLLFDGDIRIILDDLLNMEIDVFQFVQPNVVGIKTIKEKIK
                     GKRCIKCSVDMMSTLAHGTPEDIKREAEELVKNLNSPSGGFICNVLRWYRPTYPEKNV
                     IASVETFNKYRKNEKKRF"
     gene            complement(1742..2950)
                     /locus_tag="PLF90_08495"
     CDS             complement(1742..2950)
                     /locus_tag="PLF90_08495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK57348.1"
                     /translation="MKKIPKKTEIIVIGAGLMGCSIAYYLTKEKKEVVIFEKRNIASG
                     ASGRNGGQVIQLEGRDKNPETIRKRLSITKENNKILKNLEKELNFKLEYQKIGSLDLA
                     ITKEEWEDIIKTVEIQKKSGDKEIEILNRKETLKICPVLTDKVYGARYRESDGTINPF
                     FLTYGFAFNSQKNGAKIFTYTPVKKIIKENKKVKGVELENGEKVYSDIVINSTNAWSS
                     FLCPEIDILPLRQVAVVTEPVAKLPVFPMEAFIDGDAIFTTTQTKSGNLVAGGFKTQA
                     RERNLHYDESVEPIEISGSSAIFKRVFKELENISIIRSWSGVMAVTGDCLPCIGKYPD
                     TENLYIAAGFLNGMAYGPIIGKLVSELIVYGKTSLSIEIFKPERFYKKKINWPKFYNY
                     TILAEFFARV"
     gene            complement(3081..3749)
                     /locus_tag="PLF90_08500"
     CDS             complement(3081..3749)
                     /locus_tag="PLF90_08500"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOK57349.1"
                     /translation="MRLIEISGLIENGMWNYGDELKEEIFSGPKIIEVSNIKKDGFSA
                     HRFEMSILTGTYLETGAHILENVKTVDQVPVEELFLECSIIKLKEKISGEHIKVEELE
                     KSGIEVKEGDCLIIYTGWYKMWNKNGFVLNSPHFEYSAMDWIISKKVKILAGDIPCYD
                     DIRDPSDSKNLPNLRKLYLSGAMCLAPVINGDKVESGRSKIVIMPLRVKGVSASPSRA
                     VLIL"
     gene            complement(3931..>4040)
                     /gene="era"
                     /locus_tag="PLF90_08505"
                     /gene_synonym="bex"
                     /gene_synonym="rbaA"
                     /gene_synonym="sdgE"
                     /gene_synonym="yqfH"
     CDS             complement(3931..>4040)
                     /gene="era"
                     /locus_tag="PLF90_08505"
                     /gene_synonym="bex"
                     /gene_synonym="rbaA"
                     /gene_synonym="sdgE"
                     /gene_synonym="yqfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019242631.1"
                     /note="Era; Escherichia coli Ras-like protein; Bex;
                     Bacillus Era-complementing segment; essential protein in
                     Escherichia coli that is involved in many cellular
                     processes; GTPase; binds the cell membrane through
                     apparent C-terminal domain; mutants are arrested during
                     the cell cycle; Streptococcus pneumoniae Era binds to RNA
                     and Escherichia coli Era binds 16S rRNA and 30S ribosome;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOK57350.1"
                     /translation="ELILGKKIYLELWVKVIEDWRNKIDVFRKFGYGNF"
BASE COUNT         1271 a          761 c          436 g         1572 t
ORIGIN      
        1 aggaagtgtc catcccggga aagataatgg attttcaatt gcacctgtgg ctattattat
       61 atattttgcc tttaaaataa tatttttatt ttccctaaca ataccgataa ctttatcttt
      121 aaaaatgcca taaacaacac tatttaaata gacatctatt cctaattctt taacttcttt
      181 taataatctt tcaccaatca caaatcctct tatacctgcc atgtgctcct ttgaaccaaa
      241 aaatctgtgt atctgtttaa ataactgtcc tcctggtttt gagttctcat ctatcaataa
      301 aacatcaatt cctttctttc tcgcctcaat tgcagcagat agtcccgcag gtcctcctcc
      361 aattattacc agttcttttt cttccattct ccaagacctt tttgaaattc aactttcata
      421 ccatctctta caggagttat acatgttctt acatttggta ttccatcaac aatcataaca
      481 cagtccgtac atctgcctat tccacagaaa atccctcttg gctgtttaaa tttttctgtt
      541 tttctcaata ctcttattcc atttgcatat aaaacagaag caattgtttc tccttcataa
      601 gcagtaagtt ttttgccgtt aaaataaatg taaattttct ttctttcgga cagatcaccc
      661 aaaataggat gtttataaat tctaaaatct tttcttttca ttttttctgt atttattaaa
      721 tgtttctact gacgcaataa cattcttttc aggatatgtg ggtctatacc atcttaaaac
      781 attgcatata aaaccacctg atggcgagtt aaggttttta acaagttctt ctgcttctct
      841 tttaatatct tctggagttc catgtgcaag tgtactcatc atatctacag aacatttaat
      901 acatcttttc ccttttatct tttctttaat tgtttttatt ccaactacat ttggctgaac
      961 aaattgaaaa acatcaattt ccatattcag taaatcatca agtataattc ttatatctcc
     1021 gtcaaaaaga agtcctgtat acatattctc ctggtgaatt tttctgaata acttttcata
     1081 aaatggtctg aaatattttt gccagatttg aggattaaaa aataaaccct tatttccagc
     1141 aatttcatct attatattta ccatatgagc acctgctttt ttccatttag gaattaaaga
     1201 acagtgatat tcaaaaatgt aagaaatgac atcaagaata aaattaatat cctctacaag
     1261 ttttatgaaa aatgtttgat atccaaaaat attgtaagca ataagaaacg ctccaggatc
     1321 aatatatcca catataaatc tatcagaata attttctttt attattttac ttattctttc
     1381 aaaaaattta tccgctattt ccggggatgg gaatttataa tctgaaagat tatttttatc
     1441 agcaagaggg ttttcaatta ttatataagt tgaattttta tcaaagggaa ttttctgcca
     1501 tacattccat tcatcttttc tcaaaatttc tccatttcct aaattttttt cttctataaa
     1561 tgtccagtga tatgtaaccc atgcagaatc aaaatcttct gttcccggta taaatctcac
     1621 tttatcaggg ttaagtgttc cataagcatt gtatattttg ggtacatcaa cgagttccat
     1681 tggaagataa tctggatttt taaattctat tgctcttttt acaatttcaa tatgttccat
     1741 tttatactct tgcaaaaaat tctgccaaaa ttgtgtaatt ataaaattta ggccagttta
     1801 tttttttctt atagaatcgt tcaggtttaa agatttcaat tgataaagat gtttttccat
     1861 aaacaataag ttctgaaaca agttttccaa taattggtcc ataagccata ccatttaaaa
     1921 aacctgcagc gatgtaaaga ttttctgtat caggatactt tcctatgcat ggtaaacaat
     1981 ctcctgtaac tgccattact ccactccatg aacgaataat tgatatgttt tcaagttctt
     2041 taaaaactct cttaaaaatt gcagaactgc ctgaaatttc aattggttca actgattcgt
     2101 cataatgtaa gttcctttct cttgcttgag ttttaaaccc accagcaaca agatttccac
     2161 tttttgtttg agtagttgta aaaatagcat caccatcaat aaacgcttcc attggaaaaa
     2221 ccggtaattt tgcaaccggt tctgtaacaa cagcaacctg ccttaatgga agtatatcaa
     2281 tttcaggaca taaaaaactt gaccatgcat ttgtagaatt tataacaata tcagaataaa
     2341 ctttttctcc attttcaagt tcaactcctt taactttttt attttccttt ataatttttt
     2401 taacaggtgt atatgtaaaa atttttgctc catttttttg actgttgaag gcaaaaccat
     2461 atgttagaaa gaatggattt attgtaccat cagattctct gtatcttgcc ccataaactt
     2521 tatctgtcaa aacaggacag attttgagcg tttcttttct atttaaaatt tctatttctt
     2581 tatcacctga ttttttctga atttcaactg ttttaattat atcttcccac tcctcctttg
     2641 taattgcaag gtctaaactt cctattttct gatattcaag tttaaaattt aactcctttt
     2701 caaggttttt taaaattttg ttgttttctt ttgttattga taatcttttc cttattgttt
     2761 cagggttttt atccctccct tcaagttgta tcacctgtcc tccgtttcta ccacttgctc
     2821 ctgatgctat attccttttt tcaaaaataa ccacttcttt tttttctttt gtcagataat
     2881 atgcaattga acaccccata agccctgctc caataactat aatttctgtt ttttttggta
     2941 tttttttcac cttttatttc tatatttccc taaaattttt aaatttctct gaaaattttt
     3001 tactaatctc tcaactttaa aaatactcat aaaattcttt tttacaatat tgtaaaaaat
     3061 ttaaaaaaat tgtcaattca ttatagaata agaactgctc gagaaggaga agcagaaact
     3121 ccttttactc ttaaaggcat aattacaatt ttacttcttc cactttcaac tttatcgccg
     3181 tttataacag gagcaagaca catagcacct gagagataaa gttttcttaa atttggtaaa
     3241 ttttttgaat cggaagggtc ccttatatcg tcataacaag gaatatcacc tgcaagaatt
     3301 ttaactttct tacttataat ccagtccatt gcagaatatt caaaatgagg agaattcaag
     3361 acaaaaccat ttttattcca catcttatac catccggtat aaataattaa acaatctcct
     3421 tctttaactt caattcctga tttttcaagt tcttcaactt ttatatgttc tcctgatatt
     3481 ttttctttca attttattat tgaacattca agaaaaagtt cttcaacagg tacctggtca
     3541 acagttttca cattttcaag tatatgggct cctgtttcaa gatatgttcc tgttaagata
     3601 ctcatctcaa atctgtgagc agaaaaaccg tccttcttaa tatttgaaac ttcaataatt
     3661 ttaggtcctg aaaaaatttc ttcttttaat tcgtctccat aattccacat cccattttca
     3721 atcaatccac ttatctcaat aagtctcatc tccccctcct ttatttaaaa aaatttattt
     3781 agaaatatct gtaaatttta taggtattaa tcctatcttt tcatatatct tttatcaaac
     3841 ttacaatctt taacttcaaa tcaatgagat gaaaacttat aaaacaacat acttaaaaaa
     3901 ttttagcaaa acataccatt gatagaaaag ttaaaagttt ccatagccaa attttctgaa
     3961 aacatcaatt ttgtttctcc agtcctcaat tactttaacc cacaattcaa gatatatctt
     4021 tttccctaaa attaactcca
//
LOCUS       DAOSAT010000116         3958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_14395_length_3958_cov_3.705099, whole genome shotgun sequence.
ACCESSION   DAOSAT010000116 DAOSAT010000000
VERSION     DAOSAT010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14395_length_3958_cov_3.705099"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..284)
                     /locus_tag="PLF90_08510"
     CDS             complement(51..284)
                     /locus_tag="PLF90_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014933300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOK57351.1"
                     /translation="MSKLQIVDAEKMEKILYYLGFNKIRQKGSHIFYKHPDGRTTTIP
                     HHKGRVLSRPLIREILREIEITIDEYHQYLKKI"
     gene            complement(281..502)
                     /locus_tag="PLF90_08515"
     CDS             complement(281..502)
                     /locus_tag="PLF90_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006530293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOK57352.1"
                     /translation="MYKITAYVEKDPETNLYVGVVPGIPGAHTQAETLDELQENLIEV
                     VELCLEEMDEESKKQLPEFVGIQQVEVNK"
     gene            complement(1004..1411)
                     /locus_tag="PLF90_08520"
     CDS             complement(1004..1411)
                     /locus_tag="PLF90_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOK57353.1"
                     /translation="MKVRIIDTNIILRFLIGDVSDQLEKSKKLIERVEKREEKVYLPL
                     LCAFEVVFTLEKFYQIPRAEIEEKLSILFSLKGIQLPSKNIFLKSLKLYKKTNISFTD
                     AFVITLMKDTGIDEIYSFDNDFEKIEGIKRFDP"
     gene            complement(1392..1643)
                     /locus_tag="PLF90_08525"
     CDS             complement(1392..1643)
                     /locus_tag="PLF90_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOK57354.1"
                     /translation="MKTKISQKGQIVLPKKLRDDFNLKVGDYVEIEKENGFVKIVPVS
                     GSVLELAGSMKSKIKRSKKEVEIALTSESKEVANEGKDN"
     gene            complement(1781..2650)
                     /locus_tag="PLF90_08530"
     CDS             complement(1781..2650)
                     /locus_tag="PLF90_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOK57355.1"
                     /translation="MEDKKIKKKIVKAQKNELTEHFIYKKLASFMKDEKNKKILEDIG
                     KEELKHYEIFKKITKIDVKRNKLKFYFYAFLSRIFGLTFSLKLMETGEEFAQKEYEKI
                     TKVIPEVEWILKDENKHEKEIISLLDEEKLKYVSSIVLGLNDALVELTGALAGFTFAL
                     QKTKLIVIVGFITGIAAAMSMAASGYLSAKQEEGEKNPLKSSMYTGIAYIITVLFLLL
                     PYLVFKNVFLCLGFVMVNALIIILVFTFYVSVAKEVSFKKRFFEMASLSLSIAVINFL
                     IGILIRKGFGIDV"
     gene            2825..>3958
                     /locus_tag="PLF90_08535"
     CDS             2825..>3958
                     /locus_tag="PLF90_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOK57356.1"
                     /translation="MAHQRKRKIEKGLVDGELSQSIWKLAGPMMIAGALQDLFSMVDL
                     FFVGKLGHIEVAALAIAGTTMSVLMMLVQGIAVGTMVLIAHFTGEKNYEMSDNVLGQT
                     FIWSLIGASIMLPVSFFLVEPILKLFGATGNILVYAADYLRINFSYSLVIFLFVGISN
                     ALQGSGDARTPLYALVIGNVLNIILDPLYIMGYGPFPALGVAGSAVATVLTRGVGVIY
                     LFIHLLFGHSTIHFKIKYLKPIPFLMKRIVSIGFFASLQALIREISFLFLMRLVTSFG
                     AVTLAAYGIGSRLRSFIMVPGFGFASAAAVLVGQNLGAKKPKRAEKAAWRALRYYEFL
                     LTPIVIIFLFFAPTLVGFFNNNKEVITIGSSFLRYLAITFPFLA"
BASE COUNT         1216 a          644 c          535 g         1563 t
ORIGIN      
        1 ggcgccgaag cgctaagtta tgcttacggg atttatttat ttctatatct tcaaattttc
       61 tttaaatatt gatggtattc atcaatagta atttcaattt ctcttaaaat ttctcttatt
      121 aacggacgag atagaactct gcctttgtga tgaggaatag tagttgttct tccatctgga
      181 tgtttataaa atatatgact tcctttttgc cgaattttat taaatcctaa ataatataga
      241 attttttcca tcttttcagc atcaacaatt tgaagtttgc tcacttattt acctcaacct
      301 gctgtattcc aacaaattca ggcaattgtt ttttagattc ctcatccatt tcttctaaac
      361 atagttcaac tacctctata agattttctt gcagttcatc taatgtctct gcttgagtat
      421 gagcccctgg aattccagga acaacaccaa catacaaatt tgtttcaggg tctttttcaa
      481 cataagcagt tattttatac atcatatctc atatctcctt tttcactttt tactctctca
      541 aaatgtctat tttaaatcaa tgttcaaccc cgcggttaga tatggcgtaa tatattagcg
      601 tctttgaaaa gcgatttttt tgcttcctga atttcttttt cagatatttt aattcctttc
      661 aaagaaatgt ttttttaaat aggcaatctg gtcccttttt aattttaagg atttcttttt
      721 ctaaattttc caactttgtt aaaataccct gatttttcat gattgttccc tacattttat
      781 tataatccct ttttttaatt tttcaaccct tctaattgaa atataataaa ttgtttatct
      841 ctctcaaaac tccacttctg ctcctatctt cactgcttcc tttcttttat tatatgaatt
      901 cttgaacttc tcaaaaaact gtggagtgtt atctatatct caactttttc aagggagaaa
      961 aatttaaaca aaattattct tttcgtatag gaaattaaaa attttatggg tcaaatcgct
     1021 ttatcccttc aattttttca aagtcattat caaaagaata tatttcatct attcctgtat
     1081 ctttcattaa agttataaca aaagcatcag taaaacttat atttgttttt ttataaagtt
     1141 ttagtgattt gagaaaaata tttttagaag ggagttgtat tccttttaaa gaaaaaagga
     1201 tagatagttt ttcctctatt tctgctcgcg gaatttgata aaatttttcc agagtaaaaa
     1261 ccacctcaaa agcacaaagt aatggaagat aaactttctc ttctcttttc tctacccttt
     1321 ctataagttt tttacttttt tcaagttggt cagaaacatc tcctataaga aaccgaagaa
     1381 ttatgttagt gtcaattatc cttaccttca ttagcaacct ctttactttc tgaagttaat
     1441 gctatttcaa cttctttttt acttcttttt atcttacttt tcatagaccc tgctaattca
     1501 agaacacttc cactaacagg aacaatttta acaaatccat tttctttttc tatttcaaca
     1561 taatccccta cttttaaatt aaaatcatct cttaattttt taggtagaac tatttgccct
     1621 ttttgagata ttttcgtttt catcgtttta cctccaattt tagtattact tttatttata
     1681 gtaaaacgag attaagtttt tgtcaagagt tggagagaat agagaaaaaa attaaaaatt
     1741 taatttttgt ttcttacaaa atttttttat aggtatactt ttacacatca ataccaaagc
     1801 cctttcttat taaaattcct ataagaaaat ttataactgc aatactcaaa cttaaagatg
     1861 ccatctcaaa aaatctcttt ttaaaagaaa cttcttttgc aactgaaaca tagaaagtga
     1921 aaacaagtat aataataaga gcatttacca taacaaaacc taaacataaa aaaacatttt
     1981 taaaaacaag atatggtaga agtaaaaaaa ggacagttat aatatatgca attcctgtgt
     2041 acattgacga ttttaaagga tttttctccc cttcttcctg ttttgctgat aaatagccag
     2101 aagcagccat tgacatagca gcggcaattc ctgttataaa tccaacaata actatcaatt
     2161 ttgttttctg taaggcaaat gtaaaaccag caagagcccc tgttaattca acaagagcat
     2221 catttaaacc aagaactatt gaacttacat attttaattt ttcctcatct aaaagggata
     2281 taatttcttt ctcgtgcttg ttttcatctt taagtatcca ttctacttct ggtattactt
     2341 ttgttatttt ttcatattct ttttgtgcaa attcttcccc tgtctccatc aattttaaag
     2401 aaaaggtaag tccaaaaatt cttgataaaa acgcataaaa ataaaattta agtttatttc
     2461 gttttacatc aattttagtt attttcttaa aaatttcata atgttttagt tcttcttttc
     2521 caatatcttc aagaattttt ttattttttt catctttcat aaaagatgca agttttttat
     2581 aaataaaatg ctctgtcaat tcgtttttct gtgctttcac aattttcttt tttattttct
     2641 tatcttccat ttttatttcc tttcttcaat tatacaataa aacagaaaaa aattaaggta
     2701 gattaataag attgacagga aagaaatatg gaagtataac aggtaaatag ataaaaaaaa
     2761 tcccaccatt attttattta attgttatta aatttatatt gatatactga ataggaatta
     2821 taaaatggca catcaacgaa aaagaaaaat tgaaaagggt ttagtagatg gtgaactttc
     2881 ccagtcaata tggaaattag caggtcccat gatgatagca ggagcattgc aagacctttt
     2941 ttctatggtt gacctgtttt ttgttggaaa gttaggacat attgaggtgg ctgcacttgc
     3001 aattgcaggg acaactatgt ctgttctgat gatgctggtt caaggcattg cagttggaac
     3061 aatggtactt attgctcatt ttacaggaga aaaaaactat gagatgagtg ataatgtgtt
     3121 aggacagaca tttatctgga gtcttattgg tgcatctata atgttgcctg tttctttttt
     3181 tcttgtagag ccaatcctaa aactttttgg tgcaacagga aatattctgg tttatgctgc
     3241 tgactactta cgaataaatt ttagttattc tcttgttatt tttctttttg ttggaataag
     3301 taatgcttta cagggttcag gagatgcgag aacaccttta tacgcacttg taataggtaa
     3361 tgttcttaat ataattttag accctttata tattatgggt tatggtcctt ttcctgcatt
     3421 gggggttgct ggttctgctg ttgcaacagt tttaacaaga ggagttggtg ttatatatct
     3481 ttttatccat cttctttttg ggcattctac aattcatttt aaaataaaat atcttaaacc
     3541 aattccgttt cttatgaaaa gaattgtaag tatcggattt tttgcttcac ttcaggcgtt
     3601 aatccgtgaa atttcttttc tctttttaat gcgtcttgtc acatcctttg gtgctgtcac
     3661 tcttgctgct tatggaattg gttcccgact gaggtctttc ataatggtac ctggttttgg
     3721 atttgcaagt gctgctgctg ttcttgtggg acaaaattta ggagcaaaaa aaccgaaaag
     3781 agcagaaaaa gcggcatggc gtgcgcttag atactatgag tttctactta caccaatagt
     3841 tataattttc ttgttttttg ctcccacatt ggtaggattt tttaataata acaaagaagt
     3901 aattactatt ggcagttctt ttttaaggta tttagcaatt actttccctt ttcttgct
//
LOCUS       DAOSAT010000117         3767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_15433_length_3767_cov_5.154364, whole genome shotgun sequence.
ACCESSION   DAOSAT010000117 DAOSAT010000000
VERSION     DAOSAT010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15433_length_3767_cov_5.154364"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLF90_08540"
     CDS             complement(<1..714)
                     /locus_tag="PLF90_08540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979622.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOK57357.1"
                     /translation="MENIINSLKKYESVKNIVGPLILVEGVEGAGYGELVEILQSEER
                     RLGKVLEVEKDKALIQVFEGTSGLSLSEIRVRFTGKPIEFGVSPSILGRIFTGLGKVR
                     DGGPEIIPEKYLNINGSPINPTRRDYPSEFIQTGISAIDGLNSLVRGQKLPIFSGSGL
                     PHNLLATQIVKQAKILTEKEEKFAIVFAAMGITFEEAEFFQEEFRSTGAIERCVIFIN
                     LADEPAIERISTPRIALTAA"
     gene            775..1785
                     /gene="purM"
                     /locus_tag="PLF90_08545"
     CDS             775..1785
                     /gene="purM"
                     /locus_tag="PLF90_08545"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOK57358.1"
                     /translation="MNYKKAGVDEDKAGKLVEFIKSKARKTFTENVIGDIGLFGGFFK
                     IPQNYKSPVFVSSTDGVGTKILLGQEMGLFKNLGIDLVAMNCNDVSVCGAKVLFFLDY
                     IAVGELKGNREKEIIEGIVEGCKMADCSLIGGEIAQMKDVYGKDGFDLAGFCVGIAEK
                     DEIIDEKKVKKGNVLVGVSSNGVHSNGFSLIRKVFKKRDYHKYYDELGKILGEELLKS
                     TYIYSPLLFELFSLKLINASAHITGGGIEGNLIRVLPKKVKCVINKNSWEIPVIFRII
                     QKAGEILEKEMFNVFNMGIGLILIADKDNIEKIFEIVKKYNFKPYVIGEVKEGERKVE
                     IL"
     gene            1782..3086
                     /locus_tag="PLF90_08550"
     CDS             1782..3086
                     /locus_tag="PLF90_08550"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOK57359.1"
                     /translation="MKILFINPPVYDFSAYDLWSKPLGFLKIIDLFKKNGFEIFYFDF
                     MDRNHRFYENLKIKKGKFGCGNYYYEEVEKPEIYKNIPRKYKRFGLPLKIFFEFLNNL
                     KNPDFIFITTGMTYWVFGVEEIIKVVRELYPEVPIVIGGIYASFCYDDARNLGVDYIF
                     KGKDILKFACEFSSKFSVNIKIPEKIETYWDVYEKLDYLVVKTSYGCPFSCWYCGIKQ
                     IEPEYKKRDHNDVVEEIIKNVEKFKLEDIAFYDDALFFDFENHLFKIIEKITKNNKDL
                     RFHTPNGVHPKFINKSVAEFLKEKNFKTLRLSLETIDEERRKESGFKVSFPEFENAVK
                     NLVDAGFTKDEIGVYILAGLPSQNPYEVIKTIKILKNYPCKIKIAEYSPIPGTIDYEI
                     SKKLYPELPLENPLFQNNSIFPLWNFENKWEIINQLKIEVKS"
     gene            complement(3083..>3767)
                     /locus_tag="PLF90_08555"
     CDS             complement(3083..>3767)
                     /locus_tag="PLF90_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOK57360.1"
                     /translation="GLKSIVVIPEITPFEKIEKLKKLTKVIIKGKNYLESCDFAIKKA
                     KEENLTFLHPFDDPFIIAGQGTVALEILEEIQDLDCVIIPVGGGGLASGTLITIKSLK
                     PEVQVIGVQAEGACSMYKSWKEGRILELSEIKTIAEGIAVSKPGELTFEIIYKFIDDI
                     ILVNDEEILEGMRILFGDVKILSELAGSVSVAGLLSGKLKIKNKKVACIISGGNFSEK
                     HIYKILKQS"
BASE COUNT         1350 a          495 c          594 g         1328 t
ORIGIN      
        1 agcagcagtt aatgcaattc ttggagttga tattctttca attgccggtt catcagcaag
       61 attaataaat attacgcatc tttcaattgc tcctgtgctt ctaaactctt cctggaaaaa
      121 ttctgcttct tcaaaagtaa ttcccatagc agcaaaaaca atagcaaatt tttcttcctt
      181 ttcagttaaa atttttgcct gttttactat ctgggttgca agtaaattat gaggtaaccc
      241 tgaacctgaa aaaataggta gtttctgacc tcttactaaa ctgttaagtc catcaattgc
      301 tgaaatacct gtttgtataa attcagatgg ataatctctt cttgttggat ttataggaga
      361 accatttata tttagatatt tttcaggtat tatttcaggt ccaccatctc ttactttccc
      421 cagtcctgta aaaattcttc caaggataga aggagagaca ccgaattcta ttggttttcc
      481 tgtaaatcta actctgatct cacttaaact taaaccagaa gttccttcaa aaacctgaat
      541 aagtgcttta tctttttcaa cttcaagaac ttttccaagc cgtctttcct cactctgcaa
      601 aatctcaaca agttcaccat aaccagcacc ttcaactcct tcaaccagaa ttaaaggtcc
      661 tactatattt tttacacttt catatttctt taaagaattt attatgtttt ccataatgaa
      721 atagattata ataataaaaa aaatattgtc aaaaaatgga ggaaaaaaat gagtataaat
      781 tataaaaaag caggggtgga tgaggataag gcaggaaaac ttgttgaatt tataaaaagt
      841 aaagcaagaa aaacatttac agaaaatgtg attggagata ttggattatt tggtggtttt
      901 tttaaaattc cccaaaatta caaaagtcct gtttttgtat catctacaga tggagttgga
      961 acaaaaattt tactcggtca ggaaatggga ttgtttaaaa atctcggtat tgaccttgtt
     1021 gcaatgaatt gtaatgatgt ttctgtctgc ggagcaaaag ttttattttt tcttgactat
     1081 attgctgttg gagaacttaa aggaaatagg gaaaaagaaa ttatagaagg tattgttgaa
     1141 ggatgtaaaa tggcggattg ttctttaata ggaggagaaa tagcacagat gaaggatgtt
     1201 tatggaaagg atggatttga ccttgctggt ttttgtgttg gaattgctga aaaggatgaa
     1261 ataattgatg aaaaaaaggt taaaaaaggg aatgttttag ttggtgtatc ttcaaatgga
     1321 gttcacagta atggattttc acttataagg aaggttttta aaaaaagaga ttatcataaa
     1381 tattatgatg aacttggaaa aattcttgga gaggaacttt taaaatcaac atatatatat
     1441 tctcctttgc tttttgagtt attttcttta aaattgataa atgcatctgc tcatattaca
     1501 ggagggggta ttgaaggaaa tttaataagg gttttaccaa aaaaagtaaa atgtgtaata
     1561 aataaaaatt catgggaaat acctgtaatt ttcaggatta ttcagaaagc aggggaaata
     1621 ctagaaaagg aaatgtttaa tgtttttaat atgggaattg gacttatttt aattgctgat
     1681 aaagataaca tagaaaaaat ttttgaaata gtaaaaaaat ataatttcaa accatatgtt
     1741 attggtgaag ttaaagaggg tgaaagaaaa gtagaaatat tatgaaaatt ctctttataa
     1801 atcctcctgt ttatgatttc tctgcttatg atttatggtc aaaaccactt ggttttttaa
     1861 aaataataga tttatttaag aaaaatggtt ttgaaatttt ttattttgat tttatggata
     1921 gaaaccacag attttacgaa aacttaaaaa ttaaaaaagg gaaatttgga tgtggaaatt
     1981 attattatga agaagtggaa aaaccagaaa tttataaaaa tattccaaga aaatacaaaa
     2041 gatttggttt acctttaaag attttttttg aatttctgaa taatttaaaa aatccggatt
     2101 ttatttttat aacaacaggg atgacctact gggtttttgg agttgaagaa attatcaagg
     2161 tggtgagaga attatatcct gaagttccta ttgttatcgg tggaatttat gcaagtttct
     2221 gttatgatga tgcaagaaat ttaggagttg attatatttt taaagggaaa gatattttaa
     2281 agtttgcctg tgagttttct tcaaaattta gtgtaaatat taaaatacct gagaaaatag
     2341 agacatactg ggatgtgtat gaaaaacttg attatcttgt cgtaaaaacc tcttatggat
     2401 gtccattttc ctgctggtac tgtggaataa aacagattga acctgaatat aaaaaaagag
     2461 accataatga tgttgtagaa gaaataatta aaaatgttga gaaatttaaa ttagaagata
     2521 ttgcttttta tgatgatgct ttattttttg attttgaaaa tcatcttttt aaaattatag
     2581 aaaagattac caaaaataat aaagatttaa gatttcatac tccaaatggc gtccatccaa
     2641 aatttataaa taaatctgtt gctgaatttt taaaagagaa aaattttaaa actctccgtc
     2701 tttcacttga gacgattgat gaagaaagaa gaaaagaatc agggtttaag gtaagttttc
     2761 ctgaatttga aaatgcagtt aaaaatttag ttgatgctgg atttacaaaa gatgaaattg
     2821 gagtttacat acttgcaggt cttccttctc aaaatcctta tgaagttata aaaactataa
     2881 aaatattaaa aaattatcca tgtaagatta aaattgctga atactctcca attccaggaa
     2941 caattgatta tgaaatttca aagaaacttt atcctgaact gcctcttgaa aatccacttt
     3001 ttcaaaacaa ttcaatattc cctttatgga attttgaaaa taaatgggaa attataaacc
     3061 agttaaaaat agaagttaaa agttaagatt gttttaaaat tttgtaaata tgtttttctg
     3121 aaaaattacc tcctgagatt atgcatgcta cttttttgtt ttttattttt aatttgccac
     3181 ttaaaagtcc tgctactgaa acagaaccag cgagttctga taaaatttta acatctccaa
     3241 acagtattct cattccttct aaaatttctt catcattcac aagaattata tcatcaataa
     3301 acttataaat aatttcaaaa gtcaactctc ccggcttact aacggcaatt ccctctgcaa
     3361 ttgtttttat ttctgataat tctaaaatcc tgccttcttt ccatgattta tacattgaac
     3421 atgctccttc tgcttgaact cctattactt gaacttctgg ctttaatgac ttaattgtta
     3481 ttaaagttcc agaagcaagt cctccaccac caactgggat tataacacaa tccaggtctt
     3541 ggatttcttc taaaatttca agagcaacag ttccctgtcc tgcaattata aaagggtcat
     3601 caaatggatg aaggaaagtt aaattttctt cttttgcttt tttaatcgca aaatcacaac
     3661 tttccagata attcttacct tttataatta cttttgttag tttttttaat ttttcaattt
     3721 tttcaaaagg tgtaatttca ggaataacaa cgattgattt taaacca
//
LOCUS       DAOSAT010000118         3728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_15671_length_3728_cov_4.809965, whole genome shotgun sequence.
ACCESSION   DAOSAT010000118 DAOSAT010000000
VERSION     DAOSAT010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15671_length_3728_cov_4.809965"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="PLF90_08560"
     CDS             complement(<1..974)
                     /locus_tag="PLF90_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOK57361.1"
                     /translation="MKKIVISSGEKNIEIELNSVAKQSHGSSLVRCGETIVLVTAVQS
                     EEEKEDVDFFPLICDYREQTSAAGKIPGGFFKREGKPSEREVLVSRLIDRSIRPIFPE
                     NFYKEVQIISLVLSADQENEPDILSIIGASTSLLFSPIPFYIPVASVRIVKKNGKFLI
                     NPTISDSQESDLNLVISGTENSIVMLEGIANEISEEDFLTAIELGWNEIKKIVNVLKE
                     ITCQEKEKVEFSIEKEISEIITDYIRKNITNAYNYPEKQKRKEFYKQIKETFLTQFEE
                     EKKKEIEKIYEHIFENEIRNLILTHKKRLDGRDLKEIRPIECKVGLLP"
     gene            complement(978..1244)
                     /gene="rpsO"
                     /locus_tag="PLF90_08565"
     CDS             complement(978..1244)
                     /gene="rpsO"
                     /locus_tag="PLF90_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOK57362.1"
                     /translation="MLESEIKKDLMKKFGRHEKDTGSPEVQIAILTKRIEVLNEHFKK
                     HKKDFNSRRGFLKLIGKRRKLLRYLQQIDPEKYSKVINELNLRK"
     gene            1357..2604
                     /gene="hisS"
                     /locus_tag="PLF90_08570"
     CDS             1357..2604
                     /gene="hisS"
                     /locus_tag="PLF90_08570"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOK57363.1"
                     /translation="MENFIKLRGMRDILPEEIEIWKKIEKTFIEVSELFGFGEIRIPV
                     LESKALFEKGIGTDTDIVMKEMYEFKDKKGRDIALRPEGTASVVRAYIENKIYEKKKI
                     ARFYYLGPMFRYDRPQKGRYRQFYQFGVESFGGKSPYFDGEVIQILSIVFEKLMIENF
                     VFLINSLGCKNCKNEYVEIIKSYFEENFDKFCDDCKLRIRRNPLRVFDCKNNLCKYAI
                     EKLPSIIDYLCNDCKKHFDILINLLKKFNVNFEINPKLVRGLDYYTKTVFEVLDKNEN
                     IAIAGGGRYDNLVKEMGGPDIPAVGFAAGIDRIVPLLNSKQIEKKEIIYCIFLGENAR
                     IKGFEIMKNLIDNEIRVESDYEDRPIGEYLRIANKSGKKWCIILGENEIKKGVIIFKN
                     METGFQEEINEKDIVNIIKEKIK"
     gene            2601..>3728
                     /gene="aspS"
                     /locus_tag="PLF90_08575"
     CDS             2601..>3728
                     /gene="aspS"
                     /locus_tag="PLF90_08575"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOK57364.1"
                     /translation="MIRTHTCGELSRKNVGEKVKLCGWIHSIRDHGNLIFIDLRDRYG
                     ITQIVCSQEGISVEKWEKLKEIKNEFVISIEGYVVERPEETINKKIPTGEIEVKLINF
                     EILNTSKILPFEIEGNEKINETLRLKYRYLDMRKPDFQKNLIKRSDFVFHVREFLKKH
                     NFVEIETPILTKSTPEGARDFIVPSRLNPGKFYALPQSPQLFKQILMIGGFDRYYQIA
                     KCFRDEDLRADRQPEFTQIDIEMSFIEENDIITLMENLFKDVIEKTFGIKIEIPFKKI
                     TYNEAIEKYGTDAPDMRTNIEFVDLTRIFEKSEIRILNEIIERKGVIKGFFIKDGEKI
                     TLKNIEEYNEFVKNAGGNGIGWIRFKNDDIQSPLKKILSENI"
BASE COUNT         1340 a          429 c          618 g         1341 t
ORIGIN      
        1 ctcggtagaa gtccaacttt acactctatt ggtcttattt cttttaaatc ccttccatca
       61 agtcgttttt tatgggttag tattagattt cttatttcat tttcaaatat atgttcatat
      121 attttttcaa tttctttttt cttttcttct tcaaattgag tgagaaatgt ttctttaatc
      181 tgtttataaa attcttttct tttttgtttt tcgggataat tatacgcatt tgttatgttt
      241 tttcttatat agtcagtaat aatttcagat atttcttttt caatactgaa ctctactttt
      301 tctttttcct gacaggtaat ttcttttaag acatttacta ttttttttat ctcattccat
      361 cccagttcta tcgctgttaa aaagtcctcc tcagatattt catttgcaat tccttcaagc
      421 attactatgg agttttccgt tcctgaaatt actaaattca aatcactttc ctgtgaatca
      481 gaaattgtcg gatttattaa aaattttcca ttttttttca ctattctgac agaagcaact
      541 ggaatataaa aagggattgg agaaaaaagt aatgatgttg aagcaccaat tattgataga
      601 atatcaggtt cattttcctg gtctgctgaa agtacaagag agattatttg gacttcttta
      661 taaaaatttt ccgggaaaat tggtcttatt gacctatcaa ttaatctact tacaagaact
      721 tctctttcag atggttttcc ttctctttta aaaaatcctc ctggaatttt acctgcggct
      781 gatgtttgtt ctctgtaatc acagattaaa gggaaaaaat caacatcttc cttttcttct
      841 tcactctgga ctgctgttac aagtacaatt gtttctccac acctgacaag agaagaccca
      901 tgactttgtt ttgcaacaga attcaattca atctctatat tcttctctcc agaggagata
      961 actatttttt tcatttttta ctttctcaga ttcaactcgt taattacttt tgaatatttt
     1021 tccgggtcta tctgctggag atatcttagc aactttcttc tttttccaat caattttaaa
     1081 aatcctcttc tggaattaaa atcttttttg tgctttttaa aatgttcatt taatacttca
     1141 attctttttg taagaatagc aatttgcacc tctggagaac ctgtatcttt ttcatgtctt
     1201 ccaaattttt tcatcaaatc tttttttatc tcactttcaa gcattttttc tccctttttc
     1261 tttttttatt ttataataaa taattatatg ctaaaaaaaa cctttgtcaa ttttataatt
     1321 taatgttaaa tttattataa aattcttaag aaaaaaatgg aaaattttat aaaattgaga
     1381 ggaatgcgag atattttgcc ggaagaaatt gagatatgga agaaaattga aaaaacattt
     1441 attgaggtaa gtgagttatt tggttttgga gagataagaa ttcctgtttt agaaagtaag
     1501 gccttatttg agaagggaat tggtacagat acagatatag ttatgaagga aatgtatgaa
     1561 tttaaagata aaaaaggaag agatattgct ttaaggcctg aaggaactgc ttctgttgtc
     1621 agagcatata ttgaaaataa aatttatgaa aagaaaaaaa ttgcaagatt ttattatctt
     1681 ggtcctatgt tcaggtatga tagacctcag aaaggaagat acagacagtt ttatcagttc
     1741 ggtgtagaaa gttttggtgg aaaaagtcca tattttgatg gagaagtaat acaaatactg
     1801 agtattgttt ttgaaaaact tatgattgaa aattttgtat ttcttataaa ctctctcgga
     1861 tgcaaaaatt gtaaaaatga atatgttgaa ataataaaaa gttattttga agaaaatttt
     1921 gataaatttt gtgatgattg taaattaaga ataagaagga atccgttaag agtttttgat
     1981 tgtaagaata acttgtgtaa gtatgcaatt gaaaaattgc cctctattat tgattatttg
     2041 tgcaacgatt gtaaaaaaca ttttgatata cttataaatt tacttaaaaa atttaatgtg
     2101 aattttgaaa ttaatccgaa acttgtaaga gggcttgatt attatactaa aacagttttt
     2161 gaagtactgg ataaaaatga aaatatagca atcgcaggtg gtggaagata tgataatctt
     2221 gttaaagaaa tgggtggacc ggatattccg gctgttggtt ttgctgcagg tattgataga
     2281 atagttccgc ttttaaactc aaaacaaatt gaaaaaaagg aaattatcta ttgtattttt
     2341 cttggggaaa atgcaagaat aaaaggtttt gaaataatga aaaatttgat tgataacgaa
     2401 ataagagttg aatctgacta tgaagataga ccaataggag aatatttgag gattgctaac
     2461 aaaagcggaa aaaaatggtg tataatttta ggagaaaatg agattaaaaa aggagttatt
     2521 atttttaaaa atatggagac aggttttcag gaagaaataa atgaaaaaga tatagtgaat
     2581 attataaagg agaagataaa atgataagaa ctcatacctg cggtgaatta tcaagaaaaa
     2641 atgtcggaga aaaagtaaaa ctttgtggtt ggattcattc aatcagggac catgggaatt
     2701 taatatttat tgatttaaga gatagatatg ggattactca gattgtttgt tctcaggaag
     2761 gaatttcagt tgaaaaatgg gaaaaattaa aagaaattaa aaatgagttt gttatttcta
     2821 tagaaggtta tgttgttgaa agacctgagg agaccataaa taaaaaaata cctacaggtg
     2881 aaattgaggt taaacttatt aattttgaaa ttttaaatac aagtaaaatt ttaccatttg
     2941 aaattgaagg aaatgaaaag ataaatgaaa cattgcgact taaatataga taccttgata
     3001 tgagaaaacc tgattttcag aaaaatctta ttaaaaggtc tgatttcgtt tttcatgtaa
     3061 gagaattttt aaaaaaacat aattttgtag aaattgaaac tccgattctg acaaaaagta
     3121 ctccggaagg tgcgagagat ttcattgttc cttcaagatt gaatccgggg aaattctacg
     3181 ctttgccaca gtcaccacaa ctttttaaac agattttaat gataggaggt tttgataggt
     3241 attatcagat agcaaagtgt ttcagggatg aagatttaag agcagataga cagcctgaat
     3301 ttacacaaat agatattgaa atgtctttta tagaagaaaa tgatattatt acactgatgg
     3361 aaaatctttt taaggatgta atagaaaaaa catttggaat aaaaattgaa attccattta
     3421 aaaaaattac ttataatgag gcaatagaaa aatatggtac agatgcacct gatatgagaa
     3481 ctaacattga atttgttgat ttaaccagaa tttttgaaaa aagtgaaata aggatattga
     3541 atgaaattat tgaaagaaaa ggtgtaatta aaggtttttt tataaaagac ggagagaaga
     3601 ttactttgaa aaatattgag gaatataatg aatttgttaa aaatgctggt gggaacggaa
     3661 ttggatggat aagatttaaa aatgatgata tacaaagtcc actcaaaaaa attctttctg
     3721 aaaatata
//
LOCUS       DAOSAT010000119         3709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_15792_length_3709_cov_4.637384, whole genome shotgun sequence.
ACCESSION   DAOSAT010000119 DAOSAT010000000
VERSION     DAOSAT010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15792_length_3709_cov_4.637384"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLF90_08580"
     CDS             complement(<1..424)
                     /locus_tag="PLF90_08580"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOK57365.1"
                     /translation="MKKYFFSFFILFPTFLWSERELYIAEKVKKEIIIDGNITEEEWG
                     KKPTIKRFYIPGNFNKKPYSNTTVWIKYDDNYLYIAGKMEDKCLIGYVTKRDGPVWND
                     DVFEIFIKPEFNSNYYYEINSNILGTLFDAFFHRKGVYY"
     gene            complement(440..1756)
                     /locus_tag="PLF90_08585"
     CDS             complement(440..1756)
                     /locus_tag="PLF90_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOK57366.1"
                     /translation="MKRKNICLFFLIFITGCTNLTTKKEEVSKEKIKDIEDALFLSAE
                     NFDKRIKKLEENQSAILNVLNKNTNQISEAINSLNDYISKEKDFSGEIRKTKIEITTL
                     KEDIKSLNNKISEMKGKIDEVMVMTEEKNKKIEEGLNKKIVDIEKETSLIKKNYTDIL
                     SLPLILQKSMNEFQTSLTKVINDFQSNLLELKNSQIKLSNIIEDTSKQIKDLKTKVEI
                     LENSLKLQNKTMIDELTRQESEIFSVKREVFYITRDVKEISDKTFTKNDELFEDVNTL
                     KKTYNDLISSTSTLVKSLSNLQDEILNLKNSVAKINQNIENLNKEISDLKTEFDSTKK
                     TINENNKIFIDEFARHENEILKLKEVILSDTHISTKMNNEKIKILERDETKKIYTVQK
                     GDYLSKIAEKFKVNIDELKRVNNLKNDIIYPGQKLIIPVKINIISE"
     gene            complement(1753..2415)
                     /gene="pal"
                     /locus_tag="PLF90_08590"
     CDS             complement(1753..2415)
                     /gene="pal"
                     /locus_tag="PLF90_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOK57367.1"
                     /translation="MRKIFILGCLITFFLILTGCQNIEKKEEKVKEESIKEKISDLIT
                     KEEPEKKIDFGISEIPKVTPGIKEKKNSEVEKKEYSEEIVSENLYPIPENIKFVSPSE
                     INQEMAEIFQNIYFDYDSYDIKKEEIEILKKIGDYLIKNPEIMVLIEGHCDERGTREY
                     NLVLGEQRALSVRNFLINLGVSSKRLFTVSYGEDKPAVLGSNESAWAKNRRCEFKIGI
                     EK"
     gene            complement(2444..3109)
                     /locus_tag="PLF90_08595"
     CDS             complement(2444..3109)
                     /locus_tag="PLF90_08595"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOK57368.1"
                     /translation="MRSLKLYLFFLFFSKLIFCEDFNYLWAEYSTYFKDGIDARKKNI
                     ESAVKILDGYILYPNIEFSFIEEVINKIPEDEMGPASTIVGEKRVPGIGGGLCQVATT
                     LYNLALLSGISIRERKNHSSPVSYVSPGLDATVSKEEDVDLKIQNPYKYPLMIKVKVI
                     NNKLQMELYGKVPKKRNIKIVVENFRKTDNYFETITKRYIYDEGKLLFYEIISKDKYK
                     IDF"
     gene            complement(3106..>3709)
                     /locus_tag="PLF90_08600"
     CDS             complement(3106..>3709)
                     /locus_tag="PLF90_08600"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57369.1"
                     /translation="EMKVTKDTLKKFSTKIKISEEKINKGSENIIKFQKEGPGNLYYS
                     NLLSYYQKGFIKERNSGFKIERFYEKVVIEKKGEEIYEKYEPVREPVKVGDKIRVKLR
                     ITGEDFYEFIIIEDPLPSGFEVIEDEKNYYFYNEKQVRDEKVVFFWTEWYGEKVKEIT
                     YYIRPEIVGSFHVLPAKVYLMYFPDVYGNSDENYIKVVEK"
BASE COUNT         1110 a          583 c          365 g         1651 t
ORIGIN      
        1 tgtaataaac accctttcta tgaaagaaag catcaaataa agttccaaga atgtttgaat
       61 ttatctcata ataatagttt gagttaaatt ctggttttat gaaaatttca aaaacatcat
      121 cattccagac aggtccatcc ctttttgtga catatcctat aagacattta tcctccattt
      181 ttcctgcaat atataagtag ttgtcatcat atttaatcca gacagttgta tttgaatatg
      241 gttttttatt aaaattacca ggaatataaa atctctttat tgttggtttt ttaccccatt
      301 cttcctcagt tatgtttcca tcaattataa tttctttttt aactttttca gcaatgtata
      361 gttctctttc agaccataaa aaagttggaa atagaataaa aaatgagaaa aaatatttct
      421 tcattctttc ctactttcgt tattcagaaa ttatattaat ttttacaggt ataataagtt
      481 tttgtcctgg atagattata tcatttttca aattattaac ccttttcagt tcatctatat
      541 taactttaaa tttctctgca atttttgaaa gataatcacc tttttggact gtataaattt
      601 tttttgtttc atctctttcc aaaatcttta ttttttcatt attcattttt gttgaaatat
      661 gcgtatctga aagtataact tcttttaatt tcaaaatttc attttcatgt cttgcaaatt
      721 catcaataaa aattttatta ttttcattta tcgttttttt agttgaatca aattcggttt
      781 ttaaatcgga tatttcctta tttaaatttt caatattttg attgattttt gcaacactat
      841 tttttagatt taaaatttca tcctgtaaat tgctcagaga tttaacaagt gttgaagtgg
      901 atgagattaa gtcattatat gtttttttta gagtgttaac atcttcaaat aattcatcat
      961 tttttgtgaa ggttttgtct gatatttctt taacatctct tgttatatag aaaacttctc
     1021 ttttgaccga aaatatttcg ctttcctgtc ttgttaattc atcaatcatt gttttattct
     1081 gtaattttaa agaattttca agtatttcaa ctttagtttt taaatctttt atttgttttg
     1141 aggtatcctc aataatattt gacagtttta tctgtgaatt ttttagttct aaaagattac
     1201 tttgaaaatc atttattact tttgtaagtg aggtttggaa ttcattcatg gatttctgta
     1261 agattagagg aagtgaaagg atatctgtat agttcttttt tattagagat gtttcttttt
     1321 ctatatctac aattttttta tttagtccct cttcaatttt tttatttttt tcctcggtca
     1381 tcaccattac ttcatcaatt ttacccttca tttcacttat tttattgttt aatgacttta
     1441 tatcttcttt aagagttgta atttcaattt ttgtttttct gatttctccg gaaaaatctt
     1501 tttcctttga tatatagtca tttaaagaat taatggcttc tgaaatttgg tttgtatttt
     1561 tattcagaac atttagtatt gcggactgat tttcctctaa tttttttatt cttttatcaa
     1621 aattttctgc acttaaaaat aaagcatctt caatatcttt tattttttct ttggatactt
     1681 cttctttttt tgttgttaaa ttggtacatc cagttataaa aattagaaaa aataagcaaa
     1741 tattttttct cttcactttt ctattcctat tttgaattca catcttctat tttttgccca
     1801 tgctgactca ttacttccta aaacagcggg tttatcttca ccataactta ctgtgaacaa
     1861 tctttttgaa gatactccaa gatttatcag aaaatttctt acacttaatg ctctctgttc
     1921 tccaagaaca agattatatt ctcttgttcc tctttcatca cagtgcccct ctattaaaac
     1981 cattatttct ggatttttta taagataatc tccaattttt tttaaaattt caatttcttc
     2041 tttttttata tcataactgt cataatcaaa atatatattc tgaaagattt ctgccatttc
     2101 ctgatttatt tcagatggag atacaaattt aatattttcc ggtattggat ataaattttc
     2161 acttactatt tcttctgaat actctttttt ttccacctcg ctattctttt tttcttttat
     2221 tcccggagtg acttttggta tttctgaaat tccaaaatca atcttttttt caggttcttc
     2281 ttttgtaatt aaatcactta ttttttcttt tattgattcc tctttaactt tttcttcttt
     2341 tttttctata ttctggcatc ctgtaagaat taaaaaaaat gtgattaaac atccaagaat
     2401 aaatattttt ctcatagtta ataaattata actttcattt agtttaaaag tcaattttat
     2461 atttatcctt tgaaattatt tcataaaaca acaattttcc ctcatcgtat atatatctct
     2521 ttgtaattgt ttcaaaataa ttgtctgttt ttctaaaatt ttcaacaaca attttaatat
     2581 ttctcttttt gggtactttt ccataaagtt ccatttgaag tttattattt ataactttta
     2641 ccttaatcat caatggatat ttataaggat tttgaatttt taaatctaca tcttcttctt
     2701 ttgaaactgt agcatcaagt ccaggggaaa cataactaac aggggatgaa tgattttttc
     2761 tttctcttat tgaaattcct gaaagtaatg ctaaattgta taaggttgtt gccacctgac
     2821 ataatcctcc accaattcca ggaactcttt tttcaccaac aattgtagaa gcaggtccca
     2881 tttcatcttc aggaatttta tttataactt cttcaataaa agaaaattca atattaggat
     2941 agagaatgta tccatcaagt atttttactg cactctcaat attcttcttt cttgcatcta
     3001 ttccatcttt aaaataggta gaatattcag cccagagata attaaaatct tcacagaaaa
     3061 tcagttttga aaaaaataaa aaaaataaat ataatttcaa acttctcatt tttcaactac
     3121 ctttatatag ttttcatcag aatttccata tacatcagga aaatacatta aatatacttt
     3181 tgcaggtaaa acatgaaaag aaccaactat ttcaggtctt atataatatg taatttcctt
     3241 tactttttct ccataccatt ctgtccagaa gaatacaact ttttcatccc tcacttgttt
     3301 ttcattataa aaataataat ttttctcatc ctcaatcact tcaaaaccac ttggtaaagg
     3361 gtcttcaatt atgataaatt cataaaaatc ttctcctgtt attctcagtt ttacccgaat
     3421 tttatctcca acttttaccg gttctctcac aggttcatat ttttcataaa tttcttcccc
     3481 ttttttctca attacaactt tttcataaaa cctttcaatt ttaaaaccag aatttctttc
     3541 tttaataaaa cctttctgat aataacttaa aagatttgaa tagtataaat ttccgggtcc
     3601 ttctttttga aattttatta tattttcgct tcccttattt atcttttctt cagatatttt
     3661 aatttttgta ctgaattttt taagggtatc ttttgtgacc ttcatttct
//
LOCUS       DAOSAT010000120         3594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_16481_length_3594_cov_5.283131, whole genome shotgun sequence.
ACCESSION   DAOSAT010000120 DAOSAT010000000
VERSION     DAOSAT010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16481_length_3594_cov_5.283131"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..747
                     /locus_tag="PLF90_08605"
     CDS             46..747
                     /locus_tag="PLF90_08605"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOK57370.1"
                     /translation="MYEDYLKEDIAYLHSQWRRENPTPGWGPEIPVNTPEADIVNKEE
                     IAYKNNYVILEAEGKGHYIGCNISVTNFHGTWWGEGDDMIWVDGYKWPPDLHGTGSED
                     YFNQAWGMQNNAFLFNGSSIFEGLSVFGHNLYDGKLQGGYQTSYVFHLTNPVHFKKSI
                     KVTIEHGHGNHTSNDYSSTAYWYQIEPHKKFDILPVEKRYPVLLSFVNPENLKTEKRD
                     IIITEEMKEMKKRKK"
     gene            744..1544
                     /locus_tag="PLF90_08610"
     CDS             744..1544
                     /locus_tag="PLF90_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK57371.1"
                     /translation="MKIIDIHTHAFPDNVAEKAIPLLEEEGKIKAKLDGKISSLLFSM
                     DKYGIEKSVICSIATKITQYNSILKWSKEISSERIIPFPSFHQDDPLVFERIEEIKKE
                     GFKGIKLHPYYQKFTVDEEKMMKIYEKISKEGLILVLHTGFDFAFERIRIADPEKIMN
                     VKKNFPDLKLITTHLGAWEDWDNVEKYLIGKEIYMEISFSLEFLEKERAKRMILSHPP
                     DYILFGTDSPWTDQGETLNLLRQLKLPSDIEEKILYKNAERILFKKES"
     gene            1546..2565
                     /locus_tag="PLF90_08615"
     CDS             1546..2565
                     /locus_tag="PLF90_08615"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOK57372.1"
                     /translation="MKGAFLTKPYSIEIREIEKPEIKNSDDVLIRIKSVGVCGSDVHY
                     YKEGRIGDFVVKEPLILGHESSGIIEKVGKNVKNLKEGDRVAIEPGIPCRKCDYCKEG
                     RYNLCPDVVFFATPPVNGTFCEYIVHPSDFVYKIPENLSLDEATLIEPFSVGIHACNL
                     INLKPGEKVIVSGLGPVGLLALLSAKIYGGIVYGIEKSEKRIEFAKNFGAEYVFDTKK
                     TEIKEIKNLLPEKVDVVFECSGYSGLMEETINWVKRGGRIVFIGLGTDFVNLNISRVT
                     NEEIKISGIFRYVNTYKRAIEMISKNKINLKELISKNFPLDRVKDALEYPGKNPDSIK
                     VIVNP"
     gene            complement(2567..3580)
                     /locus_tag="PLF90_08620"
     CDS             complement(2567..3580)
                     /locus_tag="PLF90_08620"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOK57373.1"
                     /translation="MEKFIISRDDSIYEAFPDVVLLPSGKLICVFLECKHHADRSYSR
                     VVYKVSSDRGRTWSDKFHLSEVDSGWNCPRISHLSDGKVVIICDKGAGEKDIKLRKLF
                     MWYSLDEGKTWSKKIELPIPGFVPDKLKELKNGRYLIASQRYNEKGYLEQMVYISDDR
                     GKKWNGQVKIASKEGLNLCEGSILELPDGKLVTFMRENSFLGLDCFKAISYDNGETWE
                     GPYPFPIPGCHRPVSGLLKSGYILITHRFCQGGKGWLGWWTQNFFATLTDIESALSKE
                     RNQAHTRIKPIDYDRSKFSDTGYSGWVQFEDGEIYIVNYIVDDAPNGHIRGYSLTEKD
                     FFI"
BASE COUNT         1303 a          532 c          607 g         1152 t
ORIGIN      
        1 gaaggaaatt aggcatataa tcagtatttc taaattgtct acgaaattta tgaagattat
       61 ttgaaagaag atattgccta tttacacagt cagtggagaa gggaaaatcc aactcctggg
      121 tggggacctg aaatacctgt aaatactcct gaagcagata ttgttaataa agaagaaatt
      181 gcatacaaaa ataattatgt tattcttgaa gcagaaggta aagggcatta tattggatgt
      241 aatatttcag ttacaaattt tcatggaaca tggtggggtg aaggtgatga tatgatatgg
      301 gttgatgggt acaaatggcc acctgattta catggaactg gaagtgagga ctatttcaat
      361 caggcatggg gtatgcaaaa taatgctttt ctttttaatg gttcttcaat atttgaaggt
      421 ctttctgttt tcggacataa tctttatgat ggtaaattac agggcggata ccagacatcc
      481 tatgtttttc atttaacaaa tccagttcat ttcaaaaaat caataaaggt aacgatagaa
      541 cacggacatg gaaatcatac ttcaaatgat tattcttcaa ctgcatactg gtatcagata
      601 gagccacata aaaaatttga tattctacct gttgaaaaga gatatcctgt attgctttct
      661 tttgtaaatc cagaaaattt gaaaacagaa aaaagagata taataataac tgaagaaatg
      721 aaagaaatga aaaagagaaa gaaatgaaaa taatagatat acatacacat gcctttcctg
      781 ataatgtagc agaaaaagca atacctctgc ttgaagaaga gggaaaaata aaggcgaaac
      841 ttgatggaaa aatttcatct cttttatttt caatggataa atatggaatt gaaaaaagtg
      901 ttatatgttc aatagcaaca aaaattaccc agtataactc aattttaaaa tggtcaaaag
      961 agatatcctc tgaaagaatt attccatttc cttcttttca tcaggatgac ccacttgttt
     1021 ttgaaaggat agaagaaata aagaaagaag gatttaaagg tataaaactt catccatatt
     1081 atcagaaatt cacggttgat gaagaaaaaa tgatgaaaat ttatgaaaag atttcaaagg
     1141 aaggtttgat acttgttctg cataccggtt ttgattttgc ttttgagagg ataagaattg
     1201 cagaccctga aaaaattatg aatgtaaaga aaaatttccc tgacttaaaa cttattacaa
     1261 cacatcttgg agcatgggag gactgggata atgttgaaaa atacttaatt ggaaaggaaa
     1321 tttatatgga aatttctttt tctcttgaat ttcttgaaaa ggaaagggca aaaagaatga
     1381 ttttgtctca ccctcctgat tatatcttat tcggaactga ttctccatgg acagaccagg
     1441 gcgaaacatt gaatttactc agacaactta aacttccatc agatattgaa gaaaaaattt
     1501 tatataaaaa tgcagaaaga attcttttta aaaaggagag ttaaaatgaa aggtgcattt
     1561 ttgacaaagc catatagtat tgaaataagg gaaatagaaa aacctgaaat taaaaattca
     1621 gatgatgttt tgataagaat taaaagtgtt ggtgtatgcg gttcagatgt tcattattat
     1681 aaagaaggaa gaattggtga ttttgtagta aaagagccat tgattttagg acatgaaagt
     1741 tcagggataa ttgaaaaggt tggaaaaaat gtaaagaatt taaaagaagg agatagagtt
     1801 gcaatagaac caggtattcc ttgcagaaaa tgtgattact gtaaagaggg aaggtataat
     1861 ctctgtcctg atgttgtatt tttcgcaact ccaccagtaa acggtacttt ctgtgaatat
     1921 attgttcatc cttcagattt tgtttataaa ataccagaaa atttatctct tgatgaagca
     1981 acattaattg agccattttc tgttggaatt catgcctgta atttaataaa tttaaaacca
     2041 ggtgaaaaag taattgtatc tggacttgga cctgttggac ttcttgcttt actttctgca
     2101 aaaatatatg gtgggattgt ttatggaatt gaaaagagtg aaaaaaggat tgaatttgca
     2161 aaaaatttcg gtgctgaata tgtatttgat acaaaaaaga cagaaataaa agaaattaaa
     2221 aatcttttac ctgaaaaagt agatgttgtt tttgaatgtt ccggatattc aggtttaatg
     2281 gaagagacaa ttaactgggt gaaaagaggt ggaaggattg tctttattgg cttgggaaca
     2341 gattttgtca accttaatat ttccagagta acaaacgaag aaataaaaat ttcaggtatt
     2401 ttcagatatg taaacacata taaaagagca attgaaatga taagtaaaaa taaaatcaat
     2461 cttaaggagt taataagtaa aaatttccct cttgacagag tgaaagatgc gcttgaatat
     2521 cccggtaaaa atcctgacag tattaaagtt attgttaatc cctaatttag ataaaaaaat
     2581 ctttttctgt aagggaatat cctcttatat gtccattagg tgcatcatca acaatataat
     2641 taactatata aatttcacca tcttcaaact gaacccatcc cgaatatcct gtgtcagaaa
     2701 atttgcttct gtcataatca atcggtttta ttcttgtatg tgcctgattt ctttcttttg
     2761 aaagtgcaga ttcaatatct gttaatgttg caaaaaaatt ctgtgtccac catccaagcc
     2821 atccttttcc tccctgacag aatctatgag taataagaat ataaccgctt ttcaacaatc
     2881 cacttacagg tctgtggcat cctggaattg ggaagggata aggaccctcc catgtttcac
     2941 cgttatcata tgaaatcgct ttaaaacaat caagaccgag aaaactattt tctctcataa
     3001 aagttacaag tttaccatca ggaagttcaa ggatactgcc ttcacataga ttaagtcctt
     3061 cttttgatgc aattttaacc tgtccattcc attttttccc tctatcatca gaaatataaa
     3121 ccatctgttc aagataaccc ttctcattat atctctgtga agcaataaga tatcttccat
     3181 ttttaagttc ttttagttta tcaggtacaa aaccaggtat gggaagttct atttttttac
     3241 tccatgtttt accttcatcc aaactgtacc acataaaaag ttttctcaat tttatatcct
     3301 tttctcctgc tcctttatca caaattataa caacttttcc atcacttaaa tgagaaattc
     3361 tcggacagtt ccatccagag tcaacttcag aaagatgaaa tttatctgac catgttcttc
     3421 ccctatctga acttacttta taaacaactc ttgaataact tctgtctgca tgatgtttac
     3481 attcaagaaa aacacatatc aatttacccg aaggtaatag aacaacatca ggaaatgctt
     3541 cataaatact atcatccctg cttataataa acttttccat ctttcctcct tttt
//
LOCUS       DAOSAT010000121         2368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1902
            NODE_29392_length_2368_cov_4.041505, whole genome shotgun sequence.
ACCESSION   DAOSAT010000121 DAOSAT010000000
VERSION     DAOSAT010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863431
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,729
            CDSs (total)                      :: 1,689
            Genes (coding)                    :: 1,679
            CDSs (with protein)               :: 1,679
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 5 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29392_length_2368_cov_4.041505"
                     /isolate="AMR_MDS_1902"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /gene="pyrF"
                     /locus_tag="PLF90_08625"
     CDS             complement(<1..575)
                     /gene="pyrF"
                     /locus_tag="PLF90_08625"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOK57374.1"
                     /translation="MGKLIVALDLNEKEKIYELIEKIGDKVDFYKVGIISYLLFGDEL
                     IKYLKKEKKKIFLDLKFFDIPNTVENAVKIIFEKEIDMFTVHLMAGEKVIKSVVEAKK
                     KFNSETKIIGVSILTSFKEEDLQIFGINTPVKKLVEKLAEYGYNWGIDGIVCSGEELE
                     LLRSRFKSPFLLITPGIRIEEKKDDQKRITTP"
     gene            complement(550..744)
                     /locus_tag="PLF90_08630"
     CDS             complement(550..744)
                     /locus_tag="PLF90_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK57375.1"
                     /translation="MTQKQAVLFIYLLTLTTGINALILKNLNIYGGIEVLIEVILILT
                     IVATLEFFGRGKNGKTDSCS"
     gene            complement(796..1641)
                     /locus_tag="PLF90_08635"
     CDS             complement(796..1641)
                     /locus_tag="PLF90_08635"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOK57376.1"
                     /translation="MWNYLYLLSFLLSFFLSIFLTFLSGKLAKKAGIIALPSKRKIHK
                     KPTPLLGGIGIFCSFFLVIAFGMLFVNFGFVPEFIDRYIDGIYSVLPKLLSIILCGII
                     VIFFGLLDDSYELKPFQKLFFQFVIGTFVFISGIRITFFIPNIFLSYIITCSWLILMM
                     NCFNLLDNMDGLSSGIAIITGIILFTFALEMGQLFIATILSVFIGSVCGFLFHNFPPA
                     KIFMGESGSSFLGYFLGIVSILLTYYKYEESKTFLPVLIPLIVFSVPFFDTISVIVIR
                     KKRGY"
     gene            1746..2216
                     /locus_tag="PLF90_08640"
     CDS             1746..2216
                     /locus_tag="PLF90_08640"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOK57377.1"
                     /translation="MKIVIIMGSKADLEWAKKIQEKIKLFNIDVVLRVASAHKVPLKV
                     LDIINQYNNEDVVFITVAGRSNALSGFVDANTPKPVIACPPYSDKFSGIDIFSSLRMP
                     SGVAPMVVLEPEQAALSAVKILSLKYPELAKKIKEYQEKIKKEIEKQDEEVKNG"
     gene            2209..>2368
                     /locus_tag="PLF90_08645"
     CDS             2209..>2368
                     /locus_tag="PLF90_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOK57378.1"
                     /translation="MGSVKDLSVIKLPEDGKSGIGRFIFSDRYSVFDWGEMPDHIIDK
                     GKSMCISTA"
BASE COUNT          898 a          339 c          332 g          799 t
ORIGIN      
        1 ggagtagtaa ttcttttctg gtcatctttt ttttcttcaa ttcttatccc gggtgtaatt
       61 aataaaaaag gtgatttaaa tctactcctc aacagttcaa gttcttctcc agagcataca
      121 attccatcta tcccccagtt ataaccatat tcagcaagtt tttcaacaag tttttttaca
      181 ggtgtattta ttccaaatat ttgtaaatct tcttctttaa aagaggttaa tatactcact
      241 ccaataattt tggtttcact gttaaatttt tttttcgctt ccacaacact ttttattact
      301 ttctcacctg ccattagatg aactgtaaac atgtcaattt ctttttcaaa tataatttta
      361 actgcatttt ctacagtatt tggaatatca aaaaatttaa ggtcaagaaa aatttttttc
      421 ttttcttttt ttaaatattt tataagttca tcaccaaaca gaagataaga aataataccc
      481 actttataaa aatcaacttt atctccaatt ttttcaataa gttcatatat tttttccttt
      541 tcattcaaat caagagcaac tatcagtttt cccatttttt ccccttccaa aaaattcaag
      601 tgttgctact attgtaagaa ttaatattac ttcaattaaa acttcaattc ctccatatat
      661 attaagattt tttaaaatca aagcatttat acctgtggta agtgttagaa gataaatgaa
      721 taaaactgct tgtttctgtg tcatcccgag agaaacaagc cgatgtgaga gatggttttt
      781 gtctgctgta aatattcaat atcctctttt tttccttata acaattacgg atatagtgtc
      841 aaaaaatgga acagaaaaaa caatgagagg aataagaact ggtaaaaatg ttttactttc
      901 ttcatattta tagtaagtca aaagtataga aacaataccg agaaaatatc ctaaaaaact
      961 acttcctgat tctcccataa atatttttgc tggtggaaaa ttatgaaaca aaaaaccaca
     1021 aacacttcct atgaaaacag atagaattgt agcaataaat aactgtccca tttccagtgc
     1081 aaaagtaaat aaaatgatac ctgtaataat tgcaattcct gaagaaagac catccatatt
     1141 gtcaagaaga ttaaaacaat tcatcattag aattaaccaa gagcatgtaa ttatgtaaga
     1201 caaaaagata ttgggaataa aaaaagtaat tcttattccg gatataaaaa cgaaagtacc
     1261 tataacaaac tggaaaaaaa gtttttggaa aggttttagt tcataagaat catcaagtaa
     1321 accaaagaaa attactataa tcccgcatag aattatactg agcagttttg gtaaaacaga
     1381 ataaattcca tcaatatacc tatctataaa ttctggtaca aagccaaaat taacgaacaa
     1441 catcccaaat gctataacaa gaaaaaaaga acaaaatatt ccaattcctc caagaagagg
     1501 tgttggtttt ttatgtattt ttcttttgga tggtaaagca attattcctg ctttttttgc
     1561 aagtttacct gataaaaaag ttaaaaaaat agataaaaag aatgacagta aaaaagataa
     1621 caaatataaa taattccaca ttttattttc ctttttatta aattctattt tattttttct
     1681 ttatagtcaa tgaaaagaat tgcaaaatat aaaattctgg gatataattt tacagaggag
     1741 gaataatgaa aatagtcata attatgggtt ctaaagcaga tctggaatgg gcaaaaaaaa
     1801 ttcaagaaaa aataaaatta ttcaatattg atgtggtttt aagagttgct tctgcacata
     1861 aagtaccatt gaaagttctg gatatcataa atcaatataa taatgaagat gtggttttta
     1921 ttactgttgc tggaagaagt aatgctttaa gtggatttgt tgatgcaaat acaccaaaac
     1981 cagtgattgc ctgtcctcca tacagtgata aattttcagg aattgatatt ttttcaagtt
     2041 taagaatgcc ttcaggagtg gcaccaatgg ttgttcttga acctgaacaa gcagcattat
     2101 cagcagttaa aattttatct ttgaaatatc ctgaattggc aaaaaaaatt aaagaatatc
     2161 aggagaaaat aaaaaaagag atagagaaac aggatgagga ggtaaaaaat gggtagtgtt
     2221 aaggatcttt ctgttataaa attacctgaa gatggaaaaa gcggaatagg tagatttata
     2281 ttttctgata ggtattctgt ttttgactgg ggtgaaatgc ccgatcatat aatagataaa
     2341 ggaaaatcaa tgtgtatttc taccgctt
//
